LOCUS       DAORNF010000001        18384 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_2327_length_18384_cov_1.608271, whole genome shotgun sequence.
ACCESSION   DAORNF010000001 DAORNF010000000
VERSION     DAORNF010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 18384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18384
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2327_length_18384_cov_1.608271"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="PLV68_00005"
     CDS             complement(<1..210)
                     /locus_tag="PLV68_00005"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HRD99646.1"
                     /translation="MPHQHFSTPEPSVASEPISFTVAMPDGPLALTTDRGVFSHGALD
                     LGTKVLLLKSPPVPQQGHLLDLGCGS"
     gene            300..758
                     /gene="def"
                     /locus_tag="PLV68_00010"
     CDS             300..758
                     /gene="def"
                     /locus_tag="PLV68_00010"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HRD99647.1"
                     /translation="MVGKVARLVDDMFDTLFSSDSGIGLAAPQIGVQKQIFVWEIDDQ
                     PMVIINPEIAESSGEWVYDEGCLSIPGLYVEMLRPNQVLMKGLTLDGNEIEIEADELM
                     GRLFQHEVDHLHGTLMFDRMQPEQRKEALAEYRRIVEEQSQPKPRRLRLR"
     gene            755..1687
                     /locus_tag="PLV68_00015"
     CDS             755..1687
                     /locus_tag="PLV68_00015"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF012760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HRD99648.1"
                     /translation="MTLAPLPQGRPRRLVYFGTPEMAVAPLQGLVAAGFEVALVVTRA
                     DAKRGRGSALTPSPVKAAAERLGLPVSHSVDDALGAGADLGVVVAYGRLIKQHVLEAL
                     PMVNIHFSLLPRWRGAAPVERALLAGDEVTGVCLMQLDVGLDTGPVLARAEVPIGPRV
                     TLAALRDELVAAGTEMLVTNLAAGLGMGVEQQGDATYAAKIDPAELRVDWRQPAVAID
                     RLVRLGGAWTRWAGKRLKLIEVRPNPAIGVHAAEVVASGTFVGPLRVATGDGSIDLVT
                     VQPEGKPPMPAAAWANGARPQRGDTFESTSEDAG"
     gene            1684..>1915
                     /locus_tag="PLV68_00020"
     CDS             1684..>1915
                     /locus_tag="PLV68_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99649.1"
                     /translation="MTRDDGNRRDEGRRDRPKQGGAGGKGATSNQRSKGGRGHRGGGP
                     ARGRDDDQPRRHTARQVAYDALLRIDERCGGQS"
     assembly_gap    1916..1959
                     /estimated_length=44
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1960..>2751
                     /locus_tag="PLV68_00025"
     CDS             <1960..>2751
                     /locus_tag="PLV68_00025"
                     /inference="COORDINATES: protein motif:HMM:NF013216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HRD99650.1"
                     /translation="DRRFATELVYGTTRMRRACAALVERFTLTDPDPEVRTLLRLGAY
                     QIVYGNVAPHAAVSETVELAHPKVRGFVNAVLRKVVATPMVWAGEAEQLSYPDWIVER
                     YIAETGDYDATVALATMNEPPPVSERDDGYVQDLGSQWVAELVDAQPGELVLDVCAAP
                     GGKATAMAATGATVIAADRQAQRVGLVTQNAHRLPPAGRGRRRHGGAIRVGQLRPCVG
                     RCSVQRSGYVAPPGRRALAHPSRRHRRSGRDPGGSVGRSGEGRQAR"
     assembly_gap    2752..2803
                     /estimated_length=52
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2954..3499
                     /locus_tag="PLV68_00030"
     CDS             2954..3499
                     /locus_tag="PLV68_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HRD99651.1"
                     /translation="MSHDDEIQIARARRQAREFARQNEELLSAKVLTVSDGVHHGARD
                     DASGRALVAQLTDAGYAVVEHRVAPDGADSVAAALREMVAGFHGLIVTTGGTGFTPRD
                     QTPEGTRAVIEREAPGLAEAMRLVNPLGRLSRGVAGTAGEALICNTPGSSKGCVEQLG
                     AILDVLPHALRLLADPASPHP"
     gene            3611..4486
                     /gene="hisG"
                     /locus_tag="PLV68_00035"
     CDS             3611..4486
                     /gene="hisG"
                     /locus_tag="PLV68_00035"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HRD99652.1"
                     /translation="MLRLVLPKGSLERATLELFEAADLPVRRNSTVDYKATIDDPRID
                     SVRILRPQEIPSYVAEGLFDLGITGRDWVEETASTVESLGELKYSKATALPIKVVVAV
                     AGDSNVTSVADLADGVRVSTEYPELTRRFFQSHGIEADIRLSYGASEAKIPDIADVIV
                     DITETGRALRAAGLKIIDTILVSYTEVVANPAAFADPAKRHAMDQIMTLLLGTLDARG
                     RVLVKLNVAREQFEAVLALVPALKTPTVSELAHDGGFAVETVVPKDQINTLIPALKDA
                     GATGILEIPISKIIA"
     gene            4498..4764
                     /locus_tag="PLV68_00040"
     CDS             4498..4764
                     /locus_tag="PLV68_00040"
                     /inference="COORDINATES: protein motif:HMM:NF012534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HRD99653.1"
                     /translation="MSSEPTPPTGDESTGVELTGVELTGVVVAFDVDRGLGEVTVDAT
                     GERLPFHCVSIADGTRMIEVGTEVVFATMVKYAGREAIAIRPAP"
     gene            4784..5110
                     /locus_tag="PLV68_00045"
     CDS             4784..5110
                     /locus_tag="PLV68_00045"
                     /inference="COORDINATES: protein motif:HMM:NF013222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HRD99654.1"
                     /translation="MPKPPPPADAAERTARILAVIRSLREGEVVTYGDIAADAGFPRL
                     SRLVGRILAMTDDDDLPWWRVVNAVGRLVPGHEREQASLLRAEGVTCHNGRVTTAPTG
                     RFCPAP"
     gene            complement(5131..5976)
                     /locus_tag="PLV68_00050"
     CDS             complement(5131..5976)
                     /locus_tag="PLV68_00050"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HRD99655.1"
                     /translation="MAAITGRRRLWGELMTAAMTCTPSTATDAYDVDETDGRARSVIG
                     RAAIVLDAFVDDCVLSLADLTVATGLPRSTVYRFAEQLVGIGWIDRVFNGYRVGLRLF
                     EIGGRASSANRLRDCAGPWLLQAQQRSGLAVHLGVLSGSDVVYIDRLVCSRFRVPSRL
                     GARRPAHCTALGKAMLAHEPDVIVDEYVDRHLSAVTPATLTSPAAFRASLGLARTDGA
                     AIEHNEAVVGLSCVAAPVRGSGRAVGAISIVGPALGFDVRANTAIVRSAANGIWNELM
                     PSRRS"
     assembly_gap    6195..6313
                     /estimated_length=119
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6314..>6950
                     /locus_tag="PLV68_00055"
     CDS             <6314..>6950
                     /locus_tag="PLV68_00055"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRD99656.1"
                     /translation="VYEDTRHTYLGLNAVAGKPQEEWSREPMRFDNMIPGCYDAEARR
                     KDFEREGIMSSLAFPTLPRFGGALFVNFQDKELADASVKAWNDFMFDWCAVAPEMYVP
                     MAIVQLWDPPAAAKEIRRNLARGVRAITLPEETSNIGLPSYYNVDYWRPIFEVCEEAG
                     IPVCMHIASSGWKAYRPPESSAALEIALSMVATITHAVSMMYGPIPRAFPNI"
     assembly_gap    6951..6988
                     /estimated_length=38
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6989..>7319
                     /locus_tag="PLV68_00060"
     CDS             <6989..>7319
                     /locus_tag="PLV68_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99657.1"
                     /translation="GWIPVTVERADRQHGLHKYWGELDQTMKPSDICKRNMWFCMIDE
                     PYGLANRHQIGLDKIFWECDYPHANCPWPGTQALVEDLFKDVPDDEANMILFENAEKM
                     FNWKVPDL"
     assembly_gap    7320..7457
                     /estimated_length=138
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7458..>7710
                     /locus_tag="PLV68_00065"
     CDS             <7458..>7710
                     /locus_tag="PLV68_00065"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRD99658.1"
                     /translation="ERPGGGAGRDDLAFKAWNRNKRSIVLDPTSPTDREDILALIARA
                     DVFVESYGRNRSFELGLAYEDTRAVNEAIIHTSITAYGAV"
     assembly_gap    7711..7848
                     /estimated_length=138
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7849..>8103
                     /locus_tag="PLV68_00070"
     CDS             <7849..>8103
                     /locus_tag="PLV68_00070"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRD99659.1"
                     /translation="GFLSAIGTLAAIHARHVTGLGQQVDVSLLDGVLGQAPMNWWFTS
                     TEQSYLSTADKGRFGHRRVLIDQFRCADGEYLMMHTGGHG"
     assembly_gap    8104..8113
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8114..>8515
                     /locus_tag="PLV68_00075"
     CDS             <8114..>8515
                     /locus_tag="PLV68_00075"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRD99660.1"
                     /translation="VRQPFNCNNLALAAACAALDDHEFDIARRVVPTLWQNRPRDEWV
                     RLLQEHDVAAVPVLRPGEIVRHEQVEHADLVVAIPDRDYGTVQQLRPGVRFSLTPPGD
                     PQPAPTVGEHDDLLPELLARPVATHGNQRSIT"
     assembly_gap    8516..8600
                     /estimated_length=85
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8601..9586
                     /locus_tag="PLV68_00080"
     CDS             <8601..9586
                     /locus_tag="PLV68_00080"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRD99661.1"
                     /translation="PPIGDTMRTLPDPFEACQRGKRSITVDLRTDEGRAAVYQLVADA
                     DIVVHNMRPGKADKLGVGYEALRAIKPDLIYVFQPGWGSTGPWSQHKSFAPLVSAMTG
                     LMVAAAGEGNPPVPRARASEDYYGGFLGAAATLMAVRHRDRTGQGQYIESPQFHASLF
                     VVSEHMQDTDGNLLRSMTLDPEQLGIHPLYRLFQASDGWVGVAAVGDSAHRRLRDEIG
                     LDDASTPAATAAALTAVAAASTVDELVARLERAHIACEVALEVPRMPDFFWEDWALDN
                     QYVFEHHDHGTWGYMREVGLTIRLSRTPGRNAGPGPLLGEHNDEILGSLQS"
     gene            9583..10011
                     /locus_tag="PLV68_00085"
     CDS             9583..10011
                     /locus_tag="PLV68_00085"
                     /inference="COORDINATES: protein motif:HMM:NF024966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HRD99662.1"
                     /translation="MTDARRAIEGLLVAYVAGIDAGDFAAVGALFEHGEVWDTSGRVA
                     RGAEVGERIARFARIYADGTPRTRHLMSNVLIDVDEAADMATSSSSVMVLQAVEGEFP
                     LQPIFVGGYEDEFVRVDGAWRFACRRRRADLVGDLTHHRR"
     gene            complement(10030..10470)
                     /locus_tag="PLV68_00090"
     CDS             complement(10030..10470)
                     /locus_tag="PLV68_00090"
                     /inference="COORDINATES: protein motif:HMM:NF024966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HRD99663.1"
                     /translation="MPEPAAARLHDLLVVERAIERQLIGVARAMDRRDWPAFGEIARA
                     DLVADFGLDAVVDHIRRFLDRCGPTQHLLGNVVIDADLDAGTATSQAYVNDRHVGAAE
                     RAELSFETLGDYHDTWTLTDGVWRLASRTKYRRASIGTLDVFLG"
     gene            complement(10463..11395)
                     /locus_tag="PLV68_00095"
     CDS             complement(10463..11395)
                     /locus_tag="PLV68_00095"
                     /inference="COORDINATES: protein motif:HMM:NF024966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HRD99664.1"
                     /translation="MIAGHEEITAVQQVLYRYCRGLDRMDRDLVASCFAPDAHVDYLS
                     IFTGTAEAFIDEVWIRHSRYLRHSHQIGNVLVEATDDHLVSEAYVTTTLWTDTLMERK
                     VSGRYIDRWSPDPNRWVIAERIFVADQRIETPLPENPLVPLIQHARRDRDDPSYRLLA
                     PTSRPAVARVQHRLDDRRAICELKARYLNALDRNDWEAMATCLDENVTTGLRDGSYSF
                     AGRDAVVRFLRGTTLAHPLASSSHATTNPIIEFTGRLGDVVIQRQTASVLMGAGIYTD
                     EYRRGPDGWVISHTGYERLFEHRTELPTDAADDA"
     assembly_gap    11708..11832
                     /estimated_length=125
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11833..13186
                     /locus_tag="PLV68_00100"
     CDS             <11833..13186
                     /locus_tag="PLV68_00100"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRD99665.1"
                     /translation="ETFVATAPVMLDYFEKHTPLETQRVSNLHDYYKAIRDRVPGCKD
                     FSRTIEPKPFPAREMLGDWADRIAARSTLLSLGADTTLKEDETARGRGGEPVSLEVLA
                     ERERAGVRVKGAALVGALLHTIIERGGDVRVSSPAQRLVTNDEGAVIGVVVDGPDASR
                     AIGARQGVVLACGGFEWNRDMVLAYLGYDVQPLSPGGNTGDGHVMAMEVRAQMGNMGS
                     YWGQGASFDPAVVRSDGRLMPQMMRGLGAGSIIVNGRGERFTDVALTYNDFPKVFGNF
                     DEKLPGRPNLPPAWVVFGAAVRARGPILTVRPNDPTPEWMHESDTLSELAERIGVDAE
                     GLHAAVARYDADAATGVDSQFGAANPSPIGAGPYYAVQQWPATLGTNGGCRIDGDGRV
                     LGVGGEVIDGLYAAGNTTAAVLGGGYLGGGTPIACGMTFGYLAGGHVAATPRREIGAG
                     "
     gene            13345..13512
                     /locus_tag="PLV68_00105"
     CDS             13345..13512
                     /locus_tag="PLV68_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99666.1"
                     /translation="MTSAGRIGSPPAARPAKVAEVIARLIVADIVDADSLARVREAKK
                     ANPVTASSPNS"
     gene            13512..14126
                     /locus_tag="PLV68_00110"
     CDS             13512..14126
                     /locus_tag="PLV68_00110"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HRD99667.1"
                     /translation="MLERYEVGRNSLREALRLLEVSGLLRIRMGAGGGPEVADRSGDQ
                     LSRSMRLYFQTLGVTFRDVVTARRTLEPLVAREAAEHGSPEFKDRLFQLALGEANHKT
                     QFTEAAREFHDLLVSHNPSNPILALFVKAVSQVYADLVRTRGPREVRPDHDDIDAEHV
                     AIATAIADGDGDSAERLMARHLADLAEYVESQYAMSLDDVVEWT"
     gene            14260..15441
                     /locus_tag="PLV68_00115"
     CDS             14260..15441
                     /locus_tag="PLV68_00115"
                     /inference="COORDINATES: protein motif:HMM:NF019640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99668.1"
                     /translation="MPPRASRSSAEVVEAVQSVLASIAARTDEAETLRRVPGESIKEL
                     VDTDVMRLLQPKRWGGLEGDPRDFYKAVLAVAAVCGSTGWVTGVVGIHNWQLGLFDDR
                     LQAEIWGENPDELTSSSYMPGGSLTPVTGGYEVTGRWNFSSGSDHCGWAILGALHHRS
                     DAPPAAYQIVVPRTDYVVEDTWHTVGLRGTGSNDIVIEKAFVPSHRILTRDQIYNGDA
                     PGLEVNDGPLFRMPFPSVFPNVITGPIIGMAEGMLALDIEHVRTRVSRSFGKAVDGDP
                     YSVAAIGEAAREIDACRLQLLTNIGEMYEILLAGGTVPMALRTRTRRDQVIATERCVA
                     AIDNMFDRGGAGVLATSHPMQRIWRDAHAAKHHMVNTNERTLYSWAHHAMGLGTTDPV
                     V"
     gene            15455..16342
                     /locus_tag="PLV68_00120"
     CDS             15455..16342
                     /locus_tag="PLV68_00120"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRD99669.1"
                     /translation="MTRSTRPPIRSLGYLRLRSPEPHVWRRFAVDVVGMDAADVPAGL
                     GFRVDAHPYRLAIVEGERGVDAVGFAVGNEADLAEVAERVTSTGATVDVVSAAEAEAL
                     GISGAIRFADPWGSTIEVYHGPRLLPDPPVRRHVSGFKTGDLGAGHVVFGGSGVGAAL
                     DFYCDVLGFEVRNTQRRAGSTAGYPDDRIWFLACNARHHTVALFERSGPVELVHFMFE
                     VETLDDVGRAYDRALQSGLPQRLTLGRHSNDQMVSFYSSTPDGFTVEIGCDGLVVPLG
                     TPVYETTTGSLWGHERLNS"
     gene            16339..17919
                     /locus_tag="PLV68_00125"
     CDS             16339..17919
                     /locus_tag="PLV68_00125"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRD99670.1"
                     /translation="MNTNATSVPSRCEVVVVGTGAGGLVAALAAARRGLDVVVIEREP
                     HFGGTTAYSGSGIWLPGNSVSLGAGAVDDVEATREYLDAVVDESGNRELREAYLTAAA
                     PTVDELLEERWIELEWKPFPDYFPEVATASQRGRSIYPLPITRPVAAAVADTVIPTLP
                     VTRGGHDTGETMIGGQALTARLLLALADRSVPVFASTELIELHRDGDRVDAVVVRTAD
                     GERRIEVERGVILASGGFEQNIELRLQHGHPVKAGWTMGAPGGRGGALAAATAIGAAT
                     DLMDACWWTPAFATEDGPAFRVRDRGRPGSIMVDRAGRRFVNESLPYDRVGGAMAALC
                     RSGQLELPAWFIIDQTFLDRYGINGVPAGETLPAAWFEGGWCHQADSVEALAATIGVD
                     ADGLVATVDDHNQACETGIDPFGRGEHAFDRFFGDPEVGPNPTLAPITKSPFYALPVV
                     LSDLGTKGGLVCDTAARVLDTSGSVIGGLYATGNAMAPWSGKVYPGPGVPIGTSIVFG
                     RLAAIDIAAASAPHGAGR"
     gene            complement(17925..>18384)
                     /locus_tag="PLV68_00130"
     CDS             complement(17925..>18384)
                     /locus_tag="PLV68_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99671.1"
                     /translation="GSRLPMSSYLVSESEVLVNPDPAPLGTRSSGFAQIAMFRRPDGQ
                     SRRHWLSRWRDHHTQVAVETQSVFEYRQNLVVDRFQIAGPVIDAIVEEGFPIEALVDR
                     NVFYAAVGSDEQLRRNQFRMSASTRTFIDHERGLDVIPTSQYVFRRPPGR"
BASE COUNT         2877 a         5890 c         6063 g         2805 t
ORIGIN      
        1 cgagccgcag ccgaggtcga gcaggtggcc ctgttgagga acgggcggtg acttgagcag
       61 cagcaccttg gtccccagat cgagcgcgcc gtggctgaat acgccgcggt cggtggtgag
      121 cgcgagcggc ccgtccggca tcgccacggt gaagctgatc ggttcactgg ccacgctcgg
      181 ctcgggggtg gagaagtgtt gatgcggcac ggggctcctg aaaagtcgcg gcgatagcct
      241 ggatacgtgg cctacagcat ccgaacctac ggcgatccgg tcttgacacg gcgtcggaca
      301 tcgtcggcaa ggtggcgcga ctggtcgacg acatgttcga cacgctgttc tccagcgaca
      361 gcggcatcgg cttggccgca ccgcagatcg gcgtgcagaa gcagatcttc gtgtgggaga
      421 tcgacgacca gccgatggtc atcatcaatc ccgagatcgc cgagagcagc ggcgagtggg
      481 tgtacgacga gggctgcctc agcatccccg gtctgtacgt cgagatgctg cgccccaacc
      541 aggtgctgat gaaggggctc acgctcgacg gcaacgagat cgagatcgaa gccgacgagc
      601 tgatgggccg cctgttccag cacgaggtcg atcacctcca cggcaccctg atgttcgacc
      661 ggatgcagcc cgaacagcgc aaggaagcgc tggccgagta tcgccgcatc gtcgaggaac
      721 agagccagcc caagccccgc cgtctgcggc tgcgatgacg ttggccccgc tgccgcaggg
      781 tcggccgcgg cggctcgtct acttcggcac gccagagatg gccgtcgccc ccttgcaggg
      841 gttggtcgcg gccggcttcg aggtcgcgct ggtggtgacc cgcgccgacg ccaagcgggg
      901 acgcggctcg gcgctgaccc ccagcccggt caaggccgcc gcagaacgat taggcctgcc
      961 ggtcagccac agcgtcgacg acgcgctcgg cgccggtgcc gacctcggtg tggtcgtcgc
     1021 ctacgggcgc ctgatcaagc agcacgtgtt ggaggcgctg ccgatggtca acatccactt
     1081 ctctctgctg ccgcgttggc gcggtgcggc gccggtcgag cgggcgttgc tcgccggtga
     1141 cgaggtgacc ggtgtgtgcc tgatgcagct cgacgtcggt ctcgacaccg gcccggtgct
     1201 cgcgcgcgcc gaggtaccga tcggcccgcg agtgaccttg gccgcgctgc gcgacgaact
     1261 ggtcgcggcc ggcaccgaga tgctcgtgac caatctggcc gccgggctcg gcatgggggt
     1321 cgagcaacag ggtgacgcga cgtacgcggc caagatcgac ccggccgagt tgcgcgtcga
     1381 ctggcgccag ccggccgtcg cgatcgaccg gctggtgcgc ctgggcggcg cgtggacgcg
     1441 gtgggccggc aaacgcctga agctgatcga ggtccgaccc aacccggcca ttggcgtcca
     1501 cgcggctgaa gtcgttgcat ccggaacgtt cgtcggcccg ctgcgggtgg cgaccggcga
     1561 cggctcgatc gacctggtca ccgtgcagcc cgagggcaag ccaccgatgc ctgccgccgc
     1621 atgggccaac ggtgcccggc ctcagcgtgg cgacacattc gaatcgacca gtgaggacgc
     1681 gggatgaccc gagacgacgg aaaccggcgt gacgagggcc ggcgtgaccg gcccaagcag
     1741 ggtggcgccg ggggtaaggg cgctaccagc aaccagcgca gcaagggcgg tcgcgggcat
     1801 cgcggtggcg gccctgcacg cggccgtgac gacgatcagc cgcggcggca caccgcacgc
     1861 caggtcgcgt acgacgcgct gttgcgcatc gacgagcggt gtggcggtca gagccnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng gaccggcgct ttgccaccga
     1981 gctcgtctac ggcaccaccc gcatgcgccg cgcctgcgcc gcgttggtcg agcggttcac
     2041 gctgaccgat cccgatcccg aggtccgcac gctgctgcgc cttggcgcgt atcagatcgt
     2101 gtacggcaac gtcgcgccgc acgccgcggt cagtgagacc gtcgagttgg cccatcccaa
     2161 ggtgcgcggc ttcgtcaacg cggtgctgcg caaggtggtg gcgacaccga tggtgtgggc
     2221 cggcgaggct gagcagctca gctaccccga ctggatcgtc gagcgctaca tcgccgagac
     2281 cggtgactac gacgccacgg tcgcgttggc gacgatgaac gagccgcccc cggtcagcga
     2341 gcgcgacgac ggctacgtgc aagacctcgg ctcgcagtgg gtggccgaac tggtcgacgc
     2401 gcagccgggt gagctcgtgc tcgacgtgtg cgccgctccg ggcggcaagg ccaccgccat
     2461 ggccgcgacg ggggcgacgg tgatcgccgc cgaccgccag gcgcagcgcg tcggactggt
     2521 cacccagaac gcccaccggt taccacctgc cggtcgtggt cgccgacggc acggcggcgc
     2581 cattcgcgtc ggccagcttc gaccgtgtgt tggtcgatgc tccgtgcagc ggtctgggta
     2641 cgttgcgccg ccgggccgac gcgcgctggc gcatccgtcc cgacgacatc gacgatctgg
     2701 tcgcgatcca ggcgggtctg ttggccgaag cggcgagggg cgtcaagccc gnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncgagtcg atcgatcacc
     2821 ccacgcccga cggcttcgac gtgatcaccg actcgctcgg cagcccgtgg cgcccgttcg
     2881 gcaagggttt ccgcttgttg cctcacgatg ccgacacaga cggcatggtg gtggttcgct
     2941 accgtgctcg atcgtgagcc acgacgacga gatccagatc gctcgggcgc ggcgtcaggc
     3001 gcgtgagttc gcgcgccaga acgaggagct gctctccgcc aaggtgttga ccgtcagcga
     3061 cggtgttcat cacggtgcgc gcgacgatgc cagtggtcgc gcgctggtcg cacaactgac
     3121 cgatgccgga tacgcggtgg tcgagcaccg tgtcgctccc gacggcgccg actcggtcgc
     3181 cgccgccttg cgcgagatgg tcgccggctt ccacggcctg atcgtcacca ccggcggtac
     3241 cggcttcacg ccccgcgatc agaccccgga aggaacgcgg gcggtgatcg aacgcgaggc
     3301 ccccggcctg gccgaagcga tgcggttggt caacccgctc ggccggttga gccgtggtgt
     3361 cgccggcacg gcaggcgagg cgctgatctg caacacgccc ggctcgtcca agggctgcgt
     3421 cgagcagctc ggcgcgatcc tcgacgtgct gccccacgcc ctgcgcctgt tggccgaccc
     3481 ggccagcccg catccgtgat cgcgctgagg ccagggatcg cgctgaggtg gggatcgcgc
     3541 tgaggccggc gtgctcgcgc tgggcaaatc ggtgggggag cgatcggaat cggctcatag
     3601 cctgacgggc gtgttacgac tggtgcttcc caagggttcg ctcgaacggg ccacgctcga
     3661 actgttcgaa gcggccgatc tacccgtgcg gcgcaactcg acggtcgact acaaagccac
     3721 catcgacgac ccgcggatcg actcggtgcg catcctgcgc ccgcaggaga tccccagcta
     3781 tgtggccgag ggactgttcg acctgggcat caccgggcgc gactgggtgg aagagaccgc
     3841 cagcacggtc gaaagcttgg gtgagttgaa gtactccaag gccaccgccc taccgatcaa
     3901 ggtggtcgtc gcggtggcgg gcgattccaa cgtcacctcg gtggccgatc tggccgacgg
     3961 ggtgcgggtc agcaccgaat accccgagct cacccggcgg ttcttccaga gccacggcat
     4021 cgaggccgac atccgcctca gctacggcgc cagcgaggcc aagatccccg acatcgccga
     4081 cgtcatcgtc gacatcaccg agaccggccg ggcgctgcgt gccgcgggcc tgaagatcat
     4141 cgacaccatc ctggtcagct acaccgaggt cgtcgccaac cccgcggcct tcgccgaccc
     4201 ggccaagcgc cacgcgatgg atcagatcat gacgctgctg ctcggcaccc tcgacgcccg
     4261 gggtcgggtg ctggtcaagc tcaacgtggc tcgcgagcag ttcgaagcgg tgctcgcgtt
     4321 ggtacctgcg ctcaagacgc caaccgtcag cgaactggcc cacgacggcg ggttcgcggt
     4381 cgagacggtg gtccccaagg atcagatcaa cacgctgatc cccgcgctca aagatgccgg
     4441 tgccaccggc atcttggaga tcccgatctc caagatcatc gcctgagcga gccgaccgtg
     4501 agcagcgagc ccaccccacc gacaggcgac gagtcgaccg gtgtcgagtt gaccggtgtc
     4561 gagttgaccg gtgttgtcgt ggcgttcgac gtcgatcgcg ggctgggcga ggtcaccgtc
     4621 gacgcgaccg gtgaacggtt gccgttccac tgcgtgtcga tcgccgacgg cacacgcatg
     4681 atcgaggtcg gcaccgaggt cgtgttcgcc accatggtca agtacgccgg ccgcgaggcg
     4741 atcgcgatcc gtcccgcgcc gtgagaccgg tgccgtgaga ccggtgccga agccgccacc
     4801 tccagccgac gcggcggaac ggacggcgcg gatcctggcg gtgatccgct cgctgcgcga
     4861 gggcgaggtc gtcacctacg gcgacatcgc cgccgacgca ggcttccccc gcctctcacg
     4921 cctggtcggg cggattctgg ccatgaccga tgacgacgac ctgccctggt ggcgcgtcgt
     4981 caacgcggta ggccgcctgg ttcccggcca cgaacgtgag caagccagcc tgctccgcgc
     5041 cgaaggcgtg acctgccaca acggtcgagt gacgaccgcc cccacaggcc gcttctgccc
     5101 cgctccctga gcgggaggtc ccgcgtgccg ttacgaccgg cgcgagggca tgagttcgtt
     5161 ccagatgccg ttcgcggcgg aacgcacgat cgccgtgttg gcgcggacat cgaacccgag
     5221 cgcgggaccg acgatcgaga tcgcccccac ggcgcgacca gaaccgcgca ccggggccgc
     5281 gacacaactc aatccgacca ccgcttcgtt gtgctcgatc gcagcaccat cggttcgtgc
     5341 cagcccaagt gaggcccgaa aggcggcggg tgatgtgagc gtcgccggcg tgaccgcgct
     5401 gagatggcga tcgacgtact cgtcgacgat gacgtcgggc tcgtgggcga gcatggcctt
     5461 gccgagggca gtgcaatgcg cggggcgacg agcaccgagg cggctcggca cgcgaaagcg
     5521 cgaacacacg aggcgatcga tgtacacgac gtcgctgccg gacaggacac cgaggtgcac
     5581 ggcgaggccg ctgcgttgct gtgcctggag cagccatggt ccagcgcagt cgcggagccg
     5641 gtttgccgac gatgcccgac cgccgatctc gaacaagcgc aggcctaccc ggtatccgtt
     5701 gaatacccgg tcgatccagc cgatgcccac caattgttcg gcgaagcggt acacggtcga
     5761 ccgtggtagc cccgtcgcga ccgtcaagtc ggcgagcgaa aggacgcagt cgtcgacgaa
     5821 ggcgtcgagc acaatcgcag cccggccgat cacggaacgg gcacgaccgt cagtttcgtc
     5881 gacgtcgtac gcgtctgttg cggtggatgg tgtgcaggtc atcgctgcgg tcatcagttc
     5941 cccccagagc cgtcggcggc ccgtgattgc ggccaccaga agtatggtca tactagttgg
     6001 agcggctcgt tctgtcaacc accgtggtgc cgttcccact gggcggcaac gaagcgttgc
     6061 cttcggtggg tgcgtgcaag atgctgctcg gcagatctcg ttgaccgttc gggggccaac
     6121 tccttccttc gcaactcccc gatccctccc cccagaaagc agagaacaca tgcgcaagct
     6181 cttcagcgtc gatgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6301 nnnnnnnnnn nnngtctacg aggacacccg ccacacgtac ctcggtctga acgcggtggc
     6361 tggcaagccg caggaggagt ggagtcggga gccgatgcgg ttcgacaaca tgatcccggg
     6421 ctgctacgac gccgaggccc gccgcaagga cttcgaacgg gaaggcatca tgtccagctt
     6481 ggcgttcccg acattgcctc gtttcggcgg cgcgctcttc gtcaacttcc aggacaagga
     6541 gttggcagac gcctccgtga aggcgtggaa cgacttcatg ttcgactggt gcgcggtggc
     6601 ccccgagatg tatgtgccga tggcgatcgt gcagctgtgg gacccgccgg cggccgcgaa
     6661 ggagatcagg cgcaaccttg ctcgcggtgt gcgcgcgatc accctcccgg aggagacgag
     6721 caacatcggt ctgccctcct actacaacgt ggactactgg cgtccgatct tcgaggtgtg
     6781 cgaggaggca ggtatcccgg tctgcatgca cattgcgtcg agcggttgga aggcctaccg
     6841 tccgccggaa tcgtcggccg cgctcgagat cgcgttgagc atggtggcca cgatcactca
     6901 tgccgtctcg atgatgtacg gaccgattcc ccgtgccttc ccgaacatca nnnnnnnnnn
     6961 nnnnnnnnnn nnnnnnnnnn nnnnnnnncg gttggattcc ggtcactgtc gagcgcgccg
     7021 atcgtcagca cggcctgcac aagtactggg gcgagctgga ccagacgatg aagccgtcgg
     7081 atatttgcaa gcggaacatg tggttctgca tgatcgacga gccgtacgga ttggcgaacc
     7141 gtcatcagat cggcctggac aagatcttct gggagtgcga ctacccccac gccaactgcc
     7201 cgtggcccgg cacccaggct ctggtcgagg acttgttcaa ggacgtgccc gacgacgagg
     7261 ccaacatgat cctgttcgag aacgccgaga agatgttcaa ctggaaagtc ccggacttgn
     7321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7441 nnnnnnnnnn nnnnnnncga acggcctggc ggtggcgccg ggcgcgacga tctcgccttc
     7501 aaggcgtgga accgcaacaa gcgcagcatc gtgctggacc ccacgtcgcc gacggatcgt
     7561 gaggacatcc tcgcgttgat cgcccgcgcc gatgtgttcg tcgagagcta tgggcgcaac
     7621 cgcagcttcg agttgggcct cgcctacgag gacacccgcg cggtcaacga ggccatcatc
     7681 cacacctcga tcacggcata cggggcggtg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncc ggcttcttgt
     7861 cggcgatcgg cacgttggcg gccatccacg cccgccatgt gaccggcctc ggtcagcagg
     7921 tcgatgtctc gctgctcgac ggtgtgttgg gtcaggcgcc gatgaactgg tggttcacgt
     7981 cgaccgagca gagctacctc agcaccgctg acaagggacg tttcggtcac cgtcgggtcc
     8041 tgatcgacca gttccgctgc gccgacggcg agtacctgat gatgcacacg ggcggccacg
     8101 gcgnnnnnnn nnntgtccgg cagccgttca actgcaacaa tctggcgctc gccgcggcat
     8161 gcgcagcgct cgacgatcac gagttcgata tcgcccgccg cgtggtcccg acgttgtggc
     8221 agaaccggcc ccgtgacgaa tgggtccggc tcctgcagga gcacgacgtg gccgcggtgc
     8281 cggttctgcg ccccggtgag atcgttcgcc acgagcaggt cgaacacgcc gacctcgtcg
     8341 tcgcgatccc cgatcgcgac tacggcaccg tgcaacagct ccgaccgggc gtgcggttct
     8401 cgctgacacc tcccggcgac ccgcagcccg ccccgacggt gggcgaacac gacgacctgc
     8461 tgccggagtt gctcgcccgg ccggtcgcca cccatggcaa tcagagatcc atcacnnnnn
     8521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8581 nnnnnnnnnn nnnnnnnnnn aaccgccgat cggtgacacg atgcggacgc tgcccgaccc
     8641 gttcgaggcc tgccaacgtg ggaagcggtc gatcaccgtc gacctgcgca ccgacgaggg
     8701 ccgcgccgcg gtgtaccaac tggtggccga cgcggacatc gtcgtgcaca acatgcgccc
     8761 gggtaaggca gacaagctcg gtgtcggcta cgaggccttg cgcgcgatca agcccgatct
     8821 catctacgtg ttccaaccag gatggggcag cacgggaccg tggtcgcagc acaagagctt
     8881 tgccccgctg gtgtccgcca tgaccggcct gatggtcgcc gcagcgggtg aaggcaaccc
     8941 acccgtgcca cgggcgcggg ccagcgagga ctactacgga gggttcctcg gcgcggccgc
     9001 gaccttgatg gctgtgcgcc accgcgaccg caccggccaa ggtcagtaca tcgaatcgcc
     9061 gcagttccac gccagcttgt tcgtcgtgtc ggaacacatg caagacaccg atggcaatct
     9121 gctacggtcg atgacgctcg accccgaaca gctcggcatt catccgctgt accggctgtt
     9181 ccaagcatcc gacggctggg ttggcgtcgc ggcggtcggc gattcggcgc accggcgtct
     9241 ccgcgacgag atcggcctcg acgacgcttc gaccccggcg gcgacggccg ccgcgctcac
     9301 cgcggtggca gcagcctcga cggtcgacga actcgtcgcc cgtctcgaac gagcgcacat
     9361 cgcctgcgag gtcgcgcttg aagttccccg catgcccgac ttcttctggg aggactgggc
     9421 gctcgacaat cagtacgtgt tcgagcatca cgatcacggc acgtggggct acatgcgcga
     9481 ggtcggcttg acgatcaggt tgtcgcgcac acccggtcgc aacgccggcc ccggtccgct
     9541 gctcggcgaa cacaacgacg agatcctggg aagcctgcag tcgtgacgga tgcgcggcgc
     9601 gcgatcgaag gtttgctcgt cgcctacgtg gctggcatcg acgccggcga cttcgcagcg
     9661 gtcggtgcgt tgttcgaaca cggcgaggtg tgggacacca gcggacgggt ggcgcgcggt
     9721 gccgaggtcg gtgagcgaat cgctcgattc gcgcggatct acgcggacgg aactcctcgc
     9781 accagacacc tgatgtcgaa cgtgttgatc gacgtggacg aggccgcgga tatggctacg
     9841 tcgagctcca gcgtgatggt cctccaagcc gtcgagggag agttcccgct gcagccgatc
     9901 ttcgtcggcg ggtacgagga cgagttcgtc cgggtcgacg gcgcgtggcg cttcgcgtgc
     9961 cggcggcgcc gcgccgatct cgtcggcgat ctgactcacc accggcgttg atcggcgccg
    10021 ggcctccgat cagccgagga acacgtcgag cgtgccgatc gacgcgcggc ggtacttggt
    10081 gcgcgacgcc agccgccaga caccgtcggt cagcgtccag gtgtcgtggt agtcgcccaa
    10141 ggtctcgaac gacagctcgg cgcgttcagc ggcgccgacg tggcggtcgt tgacgtaggc
    10201 ctgtgacgtg gctgtgcccg cgtcgaggtc ggcgtcgatc acgacattgc cgagaaggtg
    10261 ctgggtcggg ccgcaccgat ccaggaagcg acggatgtgg tcgacgaccg catcgaggcc
    10321 gaagtcggcg acgaggtcgg cgcgtgcgat ctccccgaac gcgggccaat cgcgtcggtc
    10381 catcgcccgg gcgacaccga tgagctggcg ctcgatcgcg cgctcgacga cgaggagatc
    10441 gtgcagacgc gcggccgctg gctcaggcat cgtcggccgc gtcggtcggc agctcggttc
    10501 gatgctcgaa cagtcgctcg tagccggtgt gggagatcac ccaaccgtca gggccccggc
    10561 ggtactcgtc ggtgtagatg ccagctccca tcaacaccga cgcggtctgg cgctggatca
    10621 ccacatcacc gagacgcccc gtgaactcga tgatcgggtt ggtggtcgcg tggctcgacg
    10681 atgccagcgg atgggccagg gtcgtgcccc ggaggaaccg gaccacagcg tcgcggcccg
    10741 cgaaggagta cgaaccgtcg cgcaacccgg tggtgacgtt ctcgtcgaga cacgtcgcca
    10801 tcgcctccca gtcgttgcgg tccaaggcgt tgaggtaccg cgccttgagt tcgcagatcg
    10861 ctcggcgatc gtccagccgg tgctggaccc gggcaacggc gggtcgggac gtgggcgcca
    10921 gcagccgata cgacgggtcg tcgcggtcgc gtcgggcgtg ttggatcagc ggcaccagag
    10981 ggttctccgg gagcggtgtc tcgatccgct ggtcggcgac gaagatccgc tccgcgatga
    11041 cccaacggtt gggatcaggg ctccatcggt cgatgtagcg cccgctcacc ttccgttcca
    11101 tcaaggtgtc ggtccacaag gtggtcgtca cgtaggcctc gctgacgagg tgatcgtccg
    11161 tggcctcgac aaggacgttg ccgatctgat ggctgtgacg aaggtagcgg gagtggcgga
    11221 tccacacctc gtcgatgaac gcctcggccg tgccggtgaa gatcgacagg taatcgacgt
    11281 gagcgtcggg cgcgaagcac gaggccacga gatctcgatc catccggtcc agcccgcggc
    11341 aataccgata caacacctgt tgcacggcgg tgatctcctc gtggccagca atcacggcac
    11401 gccccctctc atcgcggaat cgtgaccatg gtggcagccg gccaaaggct gaggagttac
    11461 gagtgtcact caccggaacg ggcccgtata cgacgtccgc cgaccaggac aagatgggtg
    11521 caccggggca gacaccctgt ccggaacggt ccgatcgaga tggaggcagc gtggcaacag
    11581 gcaactcgcg agtgtgggac gaagtcacgg atgtggtggt gatcggttcc ggtggggcag
    11641 ccctcgctgc agccgtgcgc gcgctgatcg acggcaaccc agtggagttc cacgggcgcg
    11701 agatcatnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11821 nnnnnnnnnn nncgagacgt tcgtcgcgac ggcgcctgtc atgctcgact acttcgagaa
    11881 gcacacgccg ctcgagacgc agcgggtgtc gaacctgcac gactactaca aggccatccg
    11941 cgaccgcgtc cctggctgca aggacttcag ccggaccatc gagcccaagc cgttcccggc
    12001 gcgcgagatg ctcggcgact gggccgatcg gatcgccgcg cgcagcaccc tgttgtcgct
    12061 cggtgcggac acgacgctca aagaagacga gaccgcccgc ggccgcggcg gcgaaccggt
    12121 gtcgctcgaa gtgctggccg agcgcgaacg cgccggggtg cgtgtcaagg gagcagccct
    12181 cgtcggcgca ctgttgcaca cgatcatcga gcgtggcgga gacgtccgcg tgtcgtcccc
    12241 ggcccagcgt ctcgtgacca acgacgaggg agcggtgatc ggtgtcgtcg tcgacggccc
    12301 ggacgccagc cgtgcgatcg gcgcccgcca aggtgtcgtg ctcgcctgtg gcggattcga
    12361 atggaatcgg gacatggtct tggcctatct gggctacgac gtgcagccct tgtcgccggg
    12421 cggcaacacg ggcgatggtc acgtcatggc gatggaggtc cgggctcaga tgggcaacat
    12481 gggctcgtac tggggccagg gggcatcgtt cgacccagcc gtcgtgcgca gcgacggtcg
    12541 gctgatgccg cagatgatgc gagggctggg cgctggttcg atcatcgtca acggtcgcgg
    12601 cgagcgtttc accgacgtcg cgctcaccta caacgacttt ccgaaggtct tcggcaactt
    12661 cgacgagaag ctgcccggcc gcccgaatct tccccccgcg tgggttgtgt tcggtgccgc
    12721 cgtgcgggct cggggcccga tcctcacggt caggccgaac gacccgacgc ccgagtggat
    12781 gcacgagagc gacacgctct cagagctcgc cgagcggatc ggtgtcgacg cggaggggct
    12841 gcacgccgcg gtcgcccgct acgacgccga tgccgcgacc ggtgtcgatt cccagttcgg
    12901 tgccgccaac ccgagcccga tcggtgctgg gccgtactac gccgtccagc agtggccggc
    12961 gacgttgggt accaacggcg gatgccggat cgacggcgat ggtcgcgtgc tgggcgtggg
    13021 cggcgaggtg atcgacggcc tgtacgccgc cgggaacacc accgctgcgg tcctcggcgg
    13081 tggctacctc ggcggcggca caccgatcgc gtgcggcatg acgttcggtt acctggcggg
    13141 cggccatgtc gcagccacgc cgcgccgtga aatcggagcc ggctgacccg ccaacccccc
    13201 gggcgggtcg acccggcccc gatgtgtccc attcagcggg acagatcggg cttggacctt
    13261 gactggggca acccggagcg ttcattctcg gtcgggctgc caacatgtcg acgagcggaa
    13321 gaatcggtgg accacaatat ggacgtgaca tcggctggtc ggatcggttc tccccccgcg
    13381 gctcggcccg ccaaggtcgc cgaggtgatc gcccggctga tcgttgccga catcgtcgat
    13441 gccgactccc tcgcgcgggt tcgcgaggcc aagaaggcca acccggtgac cgcctcgagt
    13501 ccgaactcgt gatgctcgaa cgttatgagg tcggcaggaa ctcgttgcgc gaagccctcc
    13561 ggctgctcga ggtgagtggg ttgctgcgca tccgcatggg agcaggtggc ggacccgagg
    13621 tcgccgatcg ctccggcgac cagctcagcc gatcgatgcg gctgtacttc cagaccctcg
    13681 gggtcacgtt ccgcgatgtc gtcactgccc ggcgcaccct cgagcccctc gtcgctcggg
    13741 aagcggccga acatggctct cccgagttca aggaccgctt gttccagctc gcgctcggtg
    13801 aggccaacca caagacccag ttcacggagg cagcccgcga gttccacgat ctgctcgtgt
    13861 cgcacaaccc gtcgaatccc atcctcgcgt tgttcgtgaa ggcggtgagc caggtgtacg
    13921 ccgacctcgt ccgaacgcgc gggccacgcg aagtgcggcc ggaccacgac gacatcgacg
    13981 ccgaacacgt cgcgatcgcc acggccatcg ccgatggcga cggagacagc gccgagcgct
    14041 tgatggcgcg acatctggcc gacctcgccg agtacgtcga aagtcaatac gcgatgtcgc
    14101 tcgacgacgt cgtcgagtgg acgtgacacc cgtacggctg aggtgagtcc cagtgagcgg
    14161 cactcccggg atgacaggtc gaccagcacg gcctaccctc gagatctcgg ggaagttcaa
    14221 ggattcgagc accccgtctg tccgtcgaaa gggttgaaca tgccaccacg agcgtcacgt
    14281 tcgtccgccg aagtcgtcga agcggttcag tcggtcctgg catcgatcgc ggcccgtacc
    14341 gacgaggccg agacacttcg ccgggtgccg ggggagtcga tcaaggagct cgtcgacacc
    14401 gacgtcatgc ggctgctgca gccgaagcgg tggggcggac tcgagggcga cccccgcgac
    14461 ttctacaagg ccgtcctcgc cgtcgccgcc gtgtgcggtt cgacgggatg ggtgaccggg
    14521 gtagtgggaa tccacaactg gcagctcggg ctgttcgacg accggctcca ggctgagatc
    14581 tggggagaga accccgacga gctgacctcg tcgtcctaca tgcctggcgg ctcactcact
    14641 cccgtgaccg gcggttacga agtgaccggt cggtggaact tctcgtcggg gagcgatcac
    14701 tgtggttggg cgatcctcgg tgcgctccac caccgctccg acgctccgcc ggccgcctat
    14761 cagatcgtgg tgccgcgcac cgactacgtc gtcgaggaca cgtggcacac ggtcggcctt
    14821 cgtggcaccg gcagcaacga catcgtcatc gagaaggcgt tcgtcccctc gcaccggatc
    14881 ctgacccgtg accagatcta caacggagac gctccgggcc tcgaggtcaa cgacggaccg
    14941 ttgttccgga tgccgttccc gtcggtgttc cccaacgtga tcaccgggcc gatcatcggc
    15001 atggccgagg ggatgctcgc cctcgacatc gagcatgtca ggacccgcgt cagccgctcg
    15061 ttcggcaaag ccgtcgacgg tgatccgtac tcggtggccg cgatcggtga agctgctcgc
    15121 gagatcgacg cgtgccggct gcaactgttg accaacatcg gcgagatgta cgagatcttg
    15181 ctcgccggtg gcacggtgcc gatggcgttg cggacccgga ctcgacgtga tcaggtgatc
    15241 gcgaccgagc gttgtgtcgc ggccatcgac aacatgttcg accggggcgg cgcgggcgtg
    15301 ctcgcgacct cgcatccgat gcaacggatc tggcgtgacg cgcacgcggc caagcaccac
    15361 atggtgaaca ccaacgaacg cacgctgtac agctgggcac atcacgcgat gggcctcggc
    15421 acgaccgacc cggtcgtgtg acccgcacgt gtccatgacc cgatcaaccc gcccgccgat
    15481 ccgttccctc ggatatctgc gattgcgctc gcccgagccg cacgtgtggc gacgcttcgc
    15541 cgtcgatgtc gtgggcatgg acgccgccga cgttcccgcc gggcttggct tccgcgtcga
    15601 tgcccacccg taccggctcg ccatcgtcga aggcgagcgc ggcgtcgacg cggtgggatt
    15661 cgccgtcggc aacgaagccg acctcgccga ggtggccgaa cgggtcacca gcaccggtgc
    15721 caccgtcgat gtggtatcgg ccgcggaggc cgaagcgctc ggcatcagcg gcgcgatccg
    15781 cttcgccgac ccgtggggtt ccacgatcga ggtgtaccac gggccgcggc tgctgccgga
    15841 tccgccagtg cggcgccacg tcagcggttt caagaccggc gacctcggtg ccggtcatgt
    15901 cgtcttcggc ggctccgggg tcggcgccgc gctcgacttc tactgcgacg tgctcgggtt
    15961 cgaggtccgc aacacccagc gccgtgctgg ctctacggcc ggatatcccg acgaccggat
    16021 ctggttcttg gcctgcaacg ctcgccacca caccgtcgcg ctgttcgaac gcagcggacc
    16081 cgtcgaactg gtgcacttca tgttcgaggt cgagaccctc gacgacgtcg gccgcgccta
    16141 cgaccgggcg ttgcagtcgg ggctcccgca acgactgacg ttgggtcgtc acagcaacga
    16201 ccagatggtg tcgttctact cgtcgacacc cgacggcttc acggtcgaga tcggctgcga
    16261 cggcctggtg gtcccgttgg gcacacccgt gtacgaaacg acgaccggca gcctgtgggg
    16321 ccacgaaagg ttgaactcat gaacacgaac gccacgtcgg tgccgtcgcg ttgcgaggtc
    16381 gtcgtcgtcg gtaccggcgc cggtggtctc gtggccgcac tggccgcggc gcgccgcggc
    16441 ctcgatgtcg tcgtgatcga gcgcgagcca cacttcgggg gcaccaccgc ctactccggc
    16501 tcgggcatct ggctgcccgg gaactcggtc agcctcggcg cgggcgcggt cgacgacgtc
    16561 gaggcaaccc gcgagtacct cgacgccgtg gtcgacgagt cgggcaaccg tgaactgcgc
    16621 gaggcgtatc tcaccgccgc cgcgccgacc gtcgacgagc tgttggaaga gcgttggatc
    16681 gagctggagt ggaagccgtt cccggactac ttccccgagg ttgccaccgc gtcccaacga
    16741 ggtcgttcga tctacccgtt gccgatcacg cggcccgttg ccgcggccgt ggccgacacg
    16801 gtcatcccca ccctgcccgt gacccgcgga ggacacgaca ccggcgagac gatgatcggt
    16861 ggtcaggcgc tgactgcccg cctactgctc gccctcgccg accgctcggt gcccgtcttc
    16921 gcctcgaccg aactgatcga actgcaccgt gacggtgacc gggtcgacgc cgtggtcgtg
    16981 cgcacggcgg atggcgagcg gcgcatcgag gtcgagcgcg gcgtcatcct cgcctccggc
    17041 ggattcgagc agaacatcga gctgcgcctg cagcacggtc accctgtcaa ggctggctgg
    17101 acgatgggtg cgcccggtgg ccgcggcggt gccctcgccg cggccaccgc gatcggcgcc
    17161 gcgaccgact tgatggatgc gtgctggtgg acaccggcgt tcgctaccga ggacggaccg
    17221 gcgtttcgcg tccgcgaccg gggtcgtcct ggttcgatca tggtcgaccg tgccgggcgt
    17281 cggttcgtca acgagtcact gccctacgac cgtgtcggtg gcgcgatggc tgcactgtgc
    17341 cgcagcggcc agttggaact gccggcgtgg ttcatcatcg accagacgtt tctcgaccgc
    17401 tacggcatca acggcgtccc cgccggcgag acgcttccgg cggcgtggtt cgagggtggc
    17461 tggtgccacc aggccgactc cgtcgaagcg ctggccgcga cgatcggcgt ggacgccgac
    17521 ggtctcgtcg ccaccgtcga cgaccacaat caggcgtgcg agaccgggat cgatccgttc
    17581 ggtcggggtg agcatgcctt cgatcgcttc ttcggtgatc cagaggtggg accgaacccc
    17641 acgctggccc cgatcacgaa gagcccgttc tacgcgctgc cggtcgtgtt gagcgacctc
    17701 gggaccaagg gcgggttggt gtgtgacacg gcagcccgtg tcctcgacac ctcggggagc
    17761 gtcatcggtg gcttgtacgc caccggcaac gccatggcgc cgtggagcgg aaaggtctac
    17821 ccggggcccg gtgtgccgat cggtacgtcg atcgtgttcg gccgactcgc cgcgatcgat
    17881 atcgcggcgg cgtcagcacc ccacggcgcc ggccgctgag accgtcagcg gccgggtggg
    17941 cgccggaaga cgtactggct ggtggggatg acgtcgaggc cccgctcgtg gtcgatgaac
    18001 gtgcgcgtgc tggccgacat ccggaactgg ttcctgcgca gctgctcgtc ggagccgacg
    18061 gcggcataga acacgttgcg gtcgacgagg gcctcgatgg ggaacccctc ctcgacgatc
    18121 gcgtcgatga cgggtccggc tatctggaag cgatcgacga cgaggttctg gcggtactcg
    18181 aagaccgact gggtctccac cgccacctgg gtgtggtgat cacgccaacg cgacagccag
    18241 tgtcggcgtg attggccgtc gggccgacgg aacatcgcga tctgcgcgaa cccgctgctg
    18301 cgcgtcccga gtggcgccgg gtcggggttg accagcacct cggactcgct gaccagatac
    18361 gaactcatgg gcaggcgcga accg
//
LOCUS       DAORNF010000002        16031 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_2784_length_16031_cov_1.875125, whole genome shotgun sequence.
ACCESSION   DAORNF010000002 DAORNF010000000
VERSION     DAORNF010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 16031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16031
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2784_length_16031_cov_1.875125"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(83..>316)
                     /locus_tag="PLV68_00135"
     CDS             complement(83..>316)
                     /locus_tag="PLV68_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99672.1"
                     /translation="VMWNVGCATVIAGTLLSSPATVAVGSAPLVAVLLLSMVAIRDQF
                     GAQRSVALVGYRLLLLVVLVSVPIGLALAFTRH"
     assembly_gap    317..333
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(614..1156)
                     /gene="sufT"
                     /locus_tag="PLV68_00140"
     CDS             complement(614..1156)
                     /gene="sufT"
                     /locus_tag="PLV68_00140"
                     /inference="COORDINATES: protein motif:HMM:NF013999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative Fe-S cluster assembly protein SufT"
                     /protein_id="HRD99673.1"
                     /translation="MTSWTPIELSRACAATTVPYGEQVTLDAGGLVEIVQRLGASITV
                     RTQMGSLLRIDGTDADALGLDPIGVGHRAADGPFEIDQVVDALQQIFDPEIPVSIVEL
                     GLVYRCDEIIEADGSRRIEIDMTMTAPGCGMGDVLRADALRAVQAIPAVDEVDVQIVF
                     DPPWNIGRMSEAARLQLGLM"
     gene            complement(1153..>1446)
                     /locus_tag="PLV68_00145"
     CDS             complement(1153..>1446)
                     /locus_tag="PLV68_00145"
                     /inference="COORDINATES: protein motif:HMM:NF013614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HRD99674.1"
                     /translation="QLEIYADDVKCTHGATVGRLDDDALHYLRSRGIPLVAARAMLID
                     AFLRDVTDSITHEAVRAHVAALIASAASEASEASGASGASTRATHPAGPVEHP"
     assembly_gap    1447..1543
                     /estimated_length=97
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1544..2362)
                     /locus_tag="PLV68_00150"
     CDS             complement(<1544..2362)
                     /locus_tag="PLV68_00150"
                     /inference="COORDINATES: protein motif:HMM:NF013614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HRD99675.1"
                     /translation="MSVTAHAPIPTERDERWHYAPLDEINARMERSVAPCGDVPTSVT
                     PMLIDAMVGATGARLVFVNGMNTAELSDCHQLPAGVRCDVSNTSGGGGPPVATVTVDD
                     GVRFEEPLRVVHVAVPDADEVKSLLSRARTIIELGTDAHAVVVETFGGLDGARASTMT
                     ESSTTIRLGDRAELAYYRIQIDALAATHVGHTLIDQGADSSVRMTSVTLGGDIARNAI
                     HVHLDGPGARADLAGIAVTGGHQRHDTVVTIDHAASRCTSNQRFNGVVDDHGRGA"
     gene            complement(2359..>2869)
                     /gene="sufC"
                     /locus_tag="PLV68_00155"
     CDS             complement(2359..>2869)
                     /gene="sufC"
                     /locus_tag="PLV68_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002514361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HRD99676.1"
                     /translation="LAFQYPVEIPGVGNMYFLRTAVNSIRSHRGLPELAAVEFLGLAK
                     EHMARLAMDPAFLSRSVNAGFSGGEKKRNEILQMSMLEPRLAILDETDSGLDIDALRI
                     VAQGIERLRSPHRSMLIITHHTPLLDDVQPDRVHVLHGGRIVRSGGPELAHELEADGY
                     GTLPIGVSG"
     assembly_gap    2870..2879
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2880..3115)
                     /locus_tag="PLV68_00160"
     CDS             complement(<2880..3115)
                     /locus_tag="PLV68_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRD99677.1"
                     /translation="MLHIANLYASAAGRTILDGLDLDVPTGEVHAIMGPNGSGKTTLS
                     NVLSGRDGYDVSGTVTFDGADLLPLTPEARAAAGV"
     gene            complement(3115..4554)
                     /gene="sufB"
                     /locus_tag="PLV68_00165"
     CDS             complement(3115..4554)
                     /gene="sufB"
                     /locus_tag="PLV68_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HRD99678.1"
                     /translation="MTSNMVEELVEREYAFGFHSDLDTELAPKGLSENVVRLISAKKD
                     EPDWLLEWRLGAFRHFLTLVEPKWPNLQHPPIDYQDMHYWAAPKPKPQLASLDEVDPE
                     IRATFDKLGISLDEQKRLSGVAVDAILDSVSVATTFKKTLAEAGVIFCSFSEAVREHP
                     DLVRKYLGSVVGYRDNFFAALNSAVFSDGSFCFIPAGVRCPMELSTYFRINAAQTGQF
                     ERTLIIAEEGASVSYLEGCTAPMRDENQLHAAVVELIALDDASIKYSTVQNWYPGDAD
                     GKGGIYNFVTKRGRCAGARSKISWTQVETGSAITWKYPSVILQGDDSVGEFYSVAVTT
                     NHQQADTGTKMIHIGRNTRSTILSKGISAGHAQNTYRGLVKILRSATGARNHTRCDSL
                     LIGADCGAHTFPYVEVKNDTAQVEHEASTSKISEDQLFYCRQRGLTEEDASTMIVNGF
                     CREVFDELPMEYAVEAQALMRVSLEGAVG"
     gene            4587..5042
                     /locus_tag="PLV68_00170"
     CDS             4587..5042
                     /locus_tag="PLV68_00170"
                     /inference="COORDINATES: protein motif:HMM:NF013331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group III truncated hemoglobin"
                     /protein_id="HRD99679.1"
                     /translation="MLEIFESAAWRSIRCETSHGPTPTGDLDTPGEIAELVRRFYADV
                     TQDDLLGPMFNEVARVDWSEHLPKLTAFWSRALLGIPGYQGNPFRAHLLVHAQREFTP
                     THFERWLALFHETIAGPGPTPDERSTSPTTSPVCTATSCRATRSRPARA"
     gene            5061..>5364
                     /locus_tag="PLV68_00175"
     CDS             5061..>5364
                     /locus_tag="PLV68_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99680.1"
                     /translation="MSRPAPAASAANEDIVPAEERGAAGRVAQVHAQTRRGLVMTAAF
                     VASTVVAGAAGIGDGWWLPLHLFVVGALLTAISAVTQMLAVTWSAAPAPRPDVVATQ"
     assembly_gap    5365..5425
                     /estimated_length=61
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5463..>5979
                     /locus_tag="PLV68_00180"
     CDS             5463..>5979
                     /locus_tag="PLV68_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99681.1"
                     /translation="MLCLAAILIRIRRDAVTARFAPAIEAYVSAVLAGAVGMTIGILL
                     GAGRAGSNALELREVHLTLNVYGLIGLVVAGTLPFFAATQVRSKMSTRATPAMMRTTS
                     AVLAAATGVTAVGHLAAEPIIIVSGLVVYALGLVAVGAMLPIYARKRVSWAGPRVVQL
                     VAGIAWWIAMTG"
     assembly_gap    5980..5989
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6031..>6222
                     /locus_tag="PLV68_00185"
     CDS             6031..>6222
                     /locus_tag="PLV68_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99682.1"
                     /translation="MLQALVIGGFAQILVASLAYLGPVLRGGGHRRLSAGFAITRSWT
                     SLAAGNAAALAALTGSGRAL"
     assembly_gap    6223..6333
                     /estimated_length=111
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6334..>6700
                     /locus_tag="PLV68_00190"
     CDS             <6334..>6700
                     /locus_tag="PLV68_00190"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HRD99683.1"
                     /translation="ELAAEHEHLQDLGKLLHTALHDGVDASDVFDEFVRLLQMHAAKE
                     EVGLFVHARSSTPLSDQIDALCREHDDLHRWLADGLTGPRVPDALRLLVTHIDDEEYD
                     LFPHIFHALDPEQWDDIELA"
     assembly_gap    6701..6778
                     /estimated_length=78
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6779..7583
                     /locus_tag="PLV68_00195"
     CDS             <6779..7583
                     /locus_tag="PLV68_00195"
                     /inference="COORDINATES: protein motif:HMM:NF025615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YwiC-like family protein"
                     /protein_id="HRD99684.1"
                     /translation="DATPDKVAARERSTLRAVAIPTEHGGWSLTLEPVLLGLLVAWSW
                     PGLSLGVAAMLAFLARTPLKLVLVDGWRGRWLTRTTVAAQVAGAELLVLATLVTAAAL
                     TADARFWWPLLAAAPLVAIELWFDMRSRSRRLLPELAGTVGIGSVAAAIALADGNDTV
                     VAMGLWIVVAARAAAAIPYARTQVFRTRGRPYQLWHSDVAQALAVLAVTAGWLVDAVP
                     LAAAVAIAAVAMFNVAAVRAAPRPAKIIGFQQMFFGVAVVIVTAIAVLA"
     gene            7629..8087
                     /locus_tag="PLV68_00200"
     CDS             7629..8087
                     /locus_tag="PLV68_00200"
                     /inference="COORDINATES: protein motif:HMM:NF017262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HRD99685.1"
                     /translation="MASYYIVLSLHILGATVWAGGHLVLAVTILPDALRQHRAATVSE
                     FEQRFERIGLPALAVQIVTGLWLAEHLLGSPANWFDGNSVARTVQVKLGLLVITVGLA
                     IHARFRVIPRLSDATLPTLAWHIRIVTLAAVLFVLAGASIRFGGDPVFDR"
     gene            complement(8108..9337)
                     /locus_tag="PLV68_00205"
     CDS             complement(8108..9337)
                     /locus_tag="PLV68_00205"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HRD99686.1"
                     /translation="MTIVGALAAPDAPVASRGEMDDQSERSAFPEWDPLAPHVLADQR
                     SVYDEMRGRCPVAHSDLLGWSVLRHADVLEVVHDHETYRNLVSRHLSVPNGMDPPDHT
                     SYRRLIEPYFAPGPMAQFEPVSDMIAADLVASAPRGRPIEVMDTLGREFAVRVQCAFM
                     GWSSDLHHPLRAWLDEQHRATLAEDLDALAASARRFDDVVRKVLDARRDAGADTADDP
                     STRLLGERVDGRPLTDSEIVSIVRNWTVGELGTIAASVGIIVAYLADHPDVVTRLCVD
                     RSAIPAAIDEILRIDAPLIANRRSTARPVTLAGAPLPSEARITVLWASANRDEQVFGD
                     PDEFRLDRDPALNLLYGAGIHECPGAPLARLELESFVTALLDGTERLRPVVDPASVRA
                     TYPSGGYQSAWIVLDPT"
     gene            9355..>9730
                     /locus_tag="PLV68_00210"
     CDS             9355..>9730
                     /locus_tag="PLV68_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRD99687.1"
                     /translation="MSDNGWLRMIEENPGHSSWYIERFRTMAAEGHDLDGEARFVDAM
                     VPRGARILDAGCGPGRVGGRLAALGHHVVGVDIDPELIAAAQGDHPNAIWLARDLAVL
                     DLASEGVSNRFDVIVSAGNVMTF"
     assembly_gap    9731..9752
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9753..9967
                     /locus_tag="PLV68_00215"
     CDS             <9753..9967
                     /locus_tag="PLV68_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HRD99688.1"
                     /translation="RRDVLHRLRTHLAPEGRLVVGFGADRGYDFDDFFADAASVGLRA
                     ELTLSSWDLRPFDTASAFLLAVMTAD"
     gene            10079..11128
                     /locus_tag="PLV68_00220"
     CDS             10079..11128
                     /locus_tag="PLV68_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99689.1"
                     /translation="MIPHRHDAAALDRRGVAWGLVGVAVTPAITFVGSDGLLWFDAAL
                     IGYLFGIIFMVFGVIYRYSVWVRRPSTATLNRRGWDAFRQRKAHNAAALPSLVATQLL
                     AQGFIRRRSRARWLAHQLVFWGCILAGLITFPLTMGLLHFESVGQQGDRYQVYFSRVG
                     TLRFDADSVIGWTVYHGLDIAAVLVLAGVFIFLRRRLRDPGALATERSGDFLALAGLF
                     AGSVTGLFLTVSSIWLEGRFYAALNTLHALTVILGLMYIPFGKLFHIFQRPANLGVTY
                     YKRANAEGPAAVCRRCGDGFASAQQIADLQVILPQLGFDYAAADGAGSYQDTCPKCRR
                     AQLALAQSARVGGFG"
     gene            11128..>12486
                     /locus_tag="PLV68_00225"
     CDS             11128..>12486
                     /locus_tag="PLV68_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRD99690.1"
                     /translation="MARPPLTDAELIERYGPHLNQAPPGGWNAGIEVDRVVDTHCCFC
                     GQQCGIKLKVHDNEVVGFEPWYDFPFNEGKLCPKGVKRYLQGSHPDRVVYPMERDENA
                     PGGFRRVSWDHALDRVVAEIRRIQDAYGPDSFAMLSGVSLTNEKSYLVGKFARLALHT
                     ANLDYNGRYCMVSAGAGNKKALGIDRASNPWSDIPLADVVWTAGTNIAETFPITTSYI
                     WKARDRGARLIVQDPRVVPHARTADLFLPVRPGTDSALFGAVLHELIRHDWLDHDFID
                     AHTNDFDQAAAAVADMTPAWAADITGVPKARIEQAAEWWGTSATGMLLHARGIEQQSK
                     GVDNVLAAINLGLATGKFGKPGCGVTTITGQGNGQGGREHGHKCDQLPGNRDIANPEH
                     RAHVASVWGCDVDEIPGKGIPALEIIEAIHAGEIKGLLSICFNPLVSSPDSNFTREAL
                     DRL"
     assembly_gap    12487..12496
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <12497..13357
                     /locus_tag="PLV68_00230"
     CDS             <12497..13357
                     /locus_tag="PLV68_00230"
                     /inference="COORDINATES: protein motif:HMM:NF013717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HRD99691.1"
                     /translation="VIDFFVSESGHYADVILPGLLHEEDEGTSTSGEGRIIKINAAVE
                     PPGEARRDWEILVDLADRLGSGHYFPYTDTRQIFEELRLASAGGTADYRGATWERIEA
                     EYGLFWPIPEEGHPGTPRLYEGGRFYHPDGRAKFHAVPYRLPGEVVDDDYPVWLTTGR
                     VVSQYLSGTQTRRIAALVAQYPEPLCEMHPRLAEQIGVADGDLVTVTSRRGTITLPAH
                     VVATIRPDTVFIPYHWPGDKAANQLTQRVVDPISKMPEFKVSAVRVEPAGGRVTTTDV
                     RDFDLQGGAT"
     gene            13354..13989
                     /locus_tag="PLV68_00235"
     CDS             13354..13989
                     /locus_tag="PLV68_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRD99692.1"
                     /translation="MSVDIRYGIDDSPVFVIDQSRCIGCEACVQACMECGTHRGQSLI
                     HLERIDRAATTQTAPMVCMHCEDPTCAQVCPADAIKQTEGGIVQSALKPRCIGCSNCV
                     LACPFGVPKYMAEIDQMMKCDMCTDRTSEGLKPMCASVCPSEALWYGTIDEYRENRTG
                     SLLRDFMFGRQEVRTKVYTVVDDLAAGPIDVMRGQAASWLDDPFALESGGT"
     gene            13986..14507
                     /locus_tag="PLV68_00240"
     CDS             13986..14507
                     /locus_tag="PLV68_00240"
                     /inference="COORDINATES: protein motif:HMM:NF012575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HRD99693.1"
                     /translation="MTDRRPEPQPLPVPIWKRDFPYEAAAEEEVTRREFARYLVAGAG
                     ALALGNVGLAAWTQLRTINEGEPRAIVDAASVEVGGTHLFRYSTDADPAVLLRISDTE
                     VVAFSQKCTHLGCVVYYEADEHRWHCPCHEGNFDAETGDVISGPPLRPLGRIDVETRD
                     DGMIWALGRSQET"
     gene            14715..>15140
                     /locus_tag="PLV68_00245"
     CDS             14715..>15140
                     /locus_tag="PLV68_00245"
                     /inference="COORDINATES: protein motif:HMM:NF015552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99694.1"
                     /translation="MADTPSSPIATLFPGYFALVMATGIVAIAADQQNLDLLAQGLYV
                     ITVLAYVVLAVLLASRMIRYWSNFAADVTKHAKGFAFLTTVAATNVLASASIVIHGWW
                     NLAWSLWWASLVLWAAFVYTTLIAVVTDRDKPGLQGGING"
     assembly_gap    15141..15312
                     /estimated_length=172
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <15313..15760
                     /locus_tag="PLV68_00250"
     CDS             <15313..15760
                     /locus_tag="PLV68_00250"
                     /inference="COORDINATES: protein motif:HMM:NF015552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99695.1"
                     /translation="HPLDPTEADPPAWIAAGAVAITVLAGSNILLAADQAPDRVARLA
                     PFLEGLVVTAWATATFWFPLMIAIGIWRHLVRRVPLLYHPSYWAMVFPLGMYGAATYR
                     MRAAIDLDALAWLPKIELVVALAAWTVTFVGLGWHLATSRRTTPHG"
     gene            complement(15808..>16031)
                     /locus_tag="PLV68_00255"
     CDS             complement(15808..>16031)
                     /locus_tag="PLV68_00255"
                     /inference="COORDINATES: protein motif:HMM:NF037625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99696.1"
                     /translation="LHLQAGWEQALTQRLTTRLGARADDPAPRLLAATALAVMRASLQ
                     HWLTTPHTPALPALVQSGFDQLASGLGTH"
BASE COUNT         2431 a         5287 c         5107 g         2618 t
ORIGIN      
        1 cccgcggttc ggtccgcggc agcgagctga tcgtcagcgc ttccgtcctc cgaaatcggt
       61 cgtgggatga tctcctgacg gctcagtggc gtgtgaacgc gagcgcgaga ccgatgggaa
      121 cgctcaccag aaccacgagc agcaggagcc ggtacccgac cagggcgacg gagcgttgcg
      181 ccccgaactg gtctctgatc gcaaccatcg acagcaacag cacggctacc agtggcgcgc
      241 tccctaccgc cacggtggcg gggctcgaca gcagcgtgcc ggcgatcact gtcgcgcaac
      301 cgacgttcca catcacnnnn nnnnnnnnnn nnnaagggtc cgagccgtga ctgccagata
      361 ggcctggccg gcgccgattc cgagctgggc aacacctgcg accaggacga ggaaggccgc
      421 tacccacgaa ccgtcgctcc actcggtcgg gcccgtcacc gctgcgacca ggccaccggc
      481 cacgaccgat acggtgccgg tgatcagcaa cggcaggctt cgtcgccacc ggtctgacga
      541 gaatggatcc ggcacgactt ccactctcgg tctcgacacg tctcgtggac tccgagtgtg
      601 gcggttcacc gcgtcacatc agcccgagtt ggaggcgtgc ggcctcagac atgcgcccta
      661 tgttccatgg cggatcgaac acgatctgga cgtcgacctc gtcgacggcc gggatcgctt
      721 gcaccgcgcg cagcgcatcg gcgcgcaaga cgtcgcccat gccgcagccg ggtgcggtca
      781 tggtcatgtc gatctcgatc cgacgcgagc cgtcggcttc gatgatctcg tcgcagcgat
      841 agacgagccc gagctcgacg atgctgacag ggatctccgg gtcgaagatc tgttgcaggg
      901 cgtcgacgac ctggtcgatc tcgaacggcc cgtccgccgc gcggtgtccg acaccgatcg
      961 ggtcgaggcc gagcgcgtcg gcgtctgtac cgtcgatccg cagcagcgag cccatctggg
     1021 tgcgcacggt gatgcttgct ccgagccgtt gcacgatttc gacgaggccg cccgcgtcca
     1081 gggtcacctg ttcgccatag ggcacggtcg tggccgcaca cgcgcgggac aattcgatcg
     1141 gggtccagct cgtcatgggt gctccacagg ccccgccgga tgcgttgctc tcgtcgacgc
     1201 tcccgacgct cccgacgctt ccgacgcttc cgacgcagcg gaggcgatga gcgcggcgac
     1261 gtgtgcgcgc accgcctcgt gtgtgatcga gtcggtgacg tcgcggagga atgcgtcgat
     1321 caacatcgcg cgtgccgcga cgagcggaat gccgcggctg cggaggtagt gcagtgcgtc
     1381 gtcgtcgagc cggcccacgg tcgcgccgtg ggtgcacttc acatcgtcgg cataaatctc
     1441 gagctgnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncgcgcct cgtccgtggt
     1561 cgtcgacgac accgttgaag cgttggttgc tcgtgcaccg cgaggcggca tgatcgatcg
     1621 tgacgaccgt gtcgtggcgc tggtggccac ccgtcacggc gatgcccgcg agatcggctc
     1681 gcgcccccgg tccgtcgaga tggacgtgga tcgcgttgcg ggcgatgtcg ccacccaggg
     1741 tcaccgatgt catccgtacg ctggaatcgg caccttgatc gatgagtgtg tgcccgacgt
     1801 gggtcgcagc cagcgcatcg atctgaatcc ggtagtaagc cagttcggcg cggtcaccca
     1861 gacgaattgt cgtcgaggac tcagtcatgg tcgacgctct cgccccatcg agccctccga
     1921 aggtctcgac gaccaccgcg tgggcatcgg tgcccaactc gatgatcgtg cgggcacgcg
     1981 acaacaggct cttcacctca tccgcgtcgg gtaccgcgac atgaaccacg cggagcggtt
     2041 cctcgaaccg gacgccgtcg tcgaccgtaa ccgtcgcaac gggcggcccc ccgccaccgc
     2101 tcgtgttgga gacatcacac cgcacaccgg ccggaagctg atggcagtcg gagagctcgg
     2161 cggtgttcat gccgttcacg aacaccagcc gcgccccggt cgcgccgacc atcgcgtcga
     2221 tcagcatcgg cgtcaccgag gtcggaacgt cgccacacgg cgccaccgat cgttccatgc
     2281 gtgcgttgat ctcgtcgagt ggcgcgtagt gccaccgctc gtcccgctcg gtcgggatcg
     2341 gggcatgagc ggtgacgctc accccgagac cccgatcggg agggttccgt atccgtcggc
     2401 ttcgagttcg tgggcgagct cggggccacc ggaccgcacg atccgtccac cgtggagtac
     2461 atggacgcgg tcgggttgta cgtcgtcgag gagtggggtg tggtgggtga tgatcagcat
     2521 ggagcggtgg gggctgcgga gtcgttcgat gccctgggcg acgatgcgga gggcgtcgat
     2581 gtcgagtccg gagtcggtct catcgaggat cgccagccgt ggctcgagca tcgacatctg
     2641 caggatctcg ttgcgcttct tctcgccgcc agagaagccg gcgttgaccg atcggctgag
     2701 gaacgcaggg tccatcgcca ggcgggccat gtgctccttg gcgaggccga ggaactccac
     2761 ggctgcgagc tcgggcaatc cgcggtggga gcggatcgag ttgacggcgg tacgcaggaa
     2821 gtacatgttg ccgacaccgg ggatttccac cggatactgg aaggccaggn nnnnnnnnna
     2881 cgccggccgc ggctcgcgcc tcgggtgtga gtggaaggag gtcggcgccg tcaaaggtga
     2941 ccgtgccgga gacgtcgtag ccgtctcgac cggacagcac gttcgacaac gtggttttgc
     3001 cggacccgtt ggggcccatg atggcgtgga cctcgccggt ggggacgtcg aggtcgagcc
     3061 cgtcgaggat ggtccgccct gccgctgatg cgtagaggtt ggcgatgtgc agcatcagcc
     3121 gaccgcccct tcgaggctga cccgcatcag cgcctgggcc tcgaccgcgt attccatcgg
     3181 aagctcgtcg aacacctcgc gacagaaccc gttgacgatc atggtcgatg catcctcctc
     3241 ggtgagaccg cgttggcggc agtagaacag ctggtcctcg ctgatcttcg acgtcgacgc
     3301 ctcgtgctcg acctgggcgg tgtcgttctt gacctcgacg tagggaaacg tgtgggcgcc
     3361 gcagtccgct ccgatcagga gcgagtcgca gcgggtgtgg ttgcgggcgc cggtggccga
     3421 gcgaaggatc ttcacgagac cgcgataggt gttctgcgcg tggccggccg agatgccctt
     3481 ggacaggatc gtgctgcgcg tgttgcgccc gatgtggatc atcttggtgc cggtgtcggc
     3541 ctgctggtgg ttggtcgtga ccgccaccga gtagaactca ccgaccgagt cgtcaccctg
     3601 caggatcacg ctcgggtact tccaggtgat cgcggagccg gtctcgacct gagtccagga
     3661 gatcttcgaa cgggccccgg cgcaccggcc gcgcttggtg acgaagttgt agatgccacc
     3721 tttgccatcg gcgtcgccgg gataccagtt ctgcacggtg gagtacttga tcgacgcgtc
     3781 gtcgagcgcg atcagctcga ccaccgcggc gtgcagctgg ttctcgtcgc gcatcggtgc
     3841 ggtgcagccc tcgagatagc tgacgctggc accctcttcg gcgatgatca aggtccgttc
     3901 gaactggccg gtctgcgcgg cgttgatccg gaagtacgtc gacaactcca tcgggcatcg
     3961 cacccccgcc gggatgaagc agaacgaccc gtcggagaac accgccgagt tcagcgcagc
     4021 gaagaagttg tcccggtagc cgaccaccga acccaggtac ttgcgcacca gatcgggatg
     4081 ctcgcgaacc gcttcggaga acgagcagaa gatgacgccg gcctcggcga gcgtcttctt
     4141 gaacgtggtg gccaccgaca ccgagtccag gatcgcatcg accgccaccc cggagagtcg
     4201 cttctgctcg tccaaggaga tgcccagctt gtcgaacgtg gcgcggatct cgggatcgac
     4261 ctcgtcgagg ctggcgagct gcggcttggg cttgggagcc gcccaatagt gcatgtcctg
     4321 gtagtcgatc ggcggatgct ggagattcgg ccatttcggc tccacgagcg tcaggaagtg
     4381 acggaacgca ccgagacgcc actcgagcag ccaatcgggt tcgtccttct tggccgagat
     4441 cagccgcacg acgttctcgc tcagcccctt cggcgccagc tcggtgtcga gatcgctgtg
     4501 gaacccgaac gcgtactccc gctcgacgag ctcctcgacc atgttcgatg tcacacgctc
     4561 tccttcaact agacttctca cagtgtatgc tagaaatatt cgagagtgct gcttggagat
     4621 caatacgatg cgagacatcc cacggcccga ccccgacggg tgacctggac acccccgggg
     4681 agatcgccga gttggtgcgc cggttctacg ccgacgtcac ccaggacgac ctgctcggtc
     4741 cgatgttcaa cgaggtggcg cgggtcgact ggtccgaaca cctcccgaag ctgacagcct
     4801 tctggagccg cgcgctgctc ggcatccccg gctatcaggg caacccgttc cgcgcccacc
     4861 tgctcgtcca cgcccagcgc gagttcacac cgacacactt cgagcggtgg ttggccctgt
     4921 tccacgagac gatcgctgga ccgggcccaa cgcccgacga gcgctcgaca tcgccgacaa
     4981 cgtcgcccgt gtgcacagcc accagctgtc gggccacgcg gtcacggccg gctcgggcat
     5041 agcgacctga gggtgtcgcg atgagccgac ctgcaccggc agcgagcgcc gcgaacgagg
     5101 acatcgttcc cgcggaagag cgcggcgctg ctggccgagt ggcgcaggta cacgcacaga
     5161 cccgccgcgg cctcgtcatg acggcagcgt tcgtggcgtc gaccgtggtc gctggcgccg
     5221 ctgggatcgg tgatggttgg tggctcccgc tgcacctctt cgtcgtcggc gcgttgttga
     5281 ccgcgatctc cgccgtcacg cagatgctcg ccgtcacctg gtcggccgcc cccgccccac
     5341 ggccggacgt ggtcgcgact cagcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5401 nnnnnnnnnn nnnnnnnnnn nnnnngttgt gcgtcgcagg cggagcggcg gtggtcgtcg
     5461 cgatgctctg cctcgccgcg atcctcatcc gcatccgacg cgacgcggtc accgcacgat
     5521 tcgcccccgc catcgaggcc tacgtgtccg ccgtcctcgc aggggccgtc ggcatgacga
     5581 tcgggatcct gctcggcgcc ggtcgagcag gatccaacgc tctcgagcta cgcgaggtcc
     5641 acctgaccct caacgtgtac gggctcatcg gactggtcgt cgccggcacg ttgcccttct
     5701 tcgccgcgac ccaggtgcgc tcgaagatgt cgactcgcgc cacgccggcc atgatgcgca
     5761 cgacgtccgc cgtgcttgcg gcggcgacgg gggtgaccgc cgtcggccat ctcgcggccg
     5821 aaccgatcat catcgtcagc ggactcgtcg tgtatgcgct cgggctggtc gcggtcggtg
     5881 cgatgctgcc gatctacgcc cgcaagcgcg tgtcgtgggc cggaccccga gtcgtgcagt
     5941 tggtggccgg aatcgcctgg tggatcgcca tgaccggcgn nnnnnnnnnc cctcgccgtc
     6001 acgagccgcc gcgagacgga tgaccgcgca gtgctgcagg cgctcgtgat cggcggcttc
     6061 gcgcagatcc tcgtcgcttc gctcgcctac ctcggcccgg tgctgcgcgg cggcggccat
     6121 cgacgcctga gcgccgggtt cgccatcaca cgctcgtgga cgtcgctcgc cgccggcaac
     6181 gcggccgcgc tcgctgcgct caccggctcg ggtcgggccc ttnnnnnnnn nnnnnnnnnn
     6241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngagctgg cagccgagca cgaacacctg
     6361 caggaccttg gaaagctgct gcacacagct ctccacgacg gtgtagacgc ctccgacgtg
     6421 ttcgacgagt tcgtccgcct actccagatg cacgccgcca aggaggaggt cggtctgttc
     6481 gtgcacgccc gatcgtccac tccgctcagc gaccagatcg acgcgctgtg ccgcgaacac
     6541 gacgacctgc accgctggct cgccgacggt ctcaccggac cgcgcgtacc cgacgcgctc
     6601 cggttgctgg tgacccacat cgacgacgag gagtacgacc tcttccccca catcttccac
     6661 gccctcgacc ccgagcagtg ggacgacatc gaactcgccc nnnnnnnnnn nnnnnnnnnn
     6721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnag
     6781 acgccactcc cgacaaggtc gccgcccgcg agcgatccac gctgcgagcg gtcgccatac
     6841 ccaccgagca tggcggctgg agtctcacgc tggaaccggt cctgctcggt ctgctggtcg
     6901 catggtcttg gcccggcctg tcgctcggtg tcgcggcgat gctggcgttc ctggcgcgga
     6961 cgccgctgaa gctcgtgctc gtcgatggct ggcgtggacg ctggctcact cgaaccaccg
     7021 tcgccgctca ggtcgcaggc gcggaactcc tcgtcctcgc gacgctcgtc acagccgcgg
     7081 cactcacagc cgatgcgcgg ttctggtggc cgttgctcgc cgcggcaccg ctcgtggcga
     7141 tcgagttgtg gttcgacatg cgcagccgca gccgccggct cttgcccgag ctcgccggca
     7201 ccgtcggcat cggctcggtg gccgccgcga tcgcgctcgc cgatggcaac gacacagttg
     7261 tcgcgatggg attgtggatc gtggtcgcgg cacgcgccgc agcggcgatc ccctatgcac
     7321 ggacgcaggt gttccgcacg cgtggtcggc cgtaccagct gtggcacagc gacgtcgcac
     7381 aagccctcgc cgtgctcgcg gtcaccgccg gatggctggt cgacgccgtc cctcttgcgg
     7441 cggccgtcgc aatcgccgcg gttgcgatgt tcaacgtcgc agcagtgcgc gcggcacctc
     7501 gaccggccaa gatcatcggc ttccagcaga tgttcttcgg cgtcgccgtc gtgatcgtca
     7561 ccgcgatcgc cgtgctcgcg tgaccgtccg cctgaacccg atccgtggca tgaggaggcc
     7621 gatctgagat ggcgagctac tacatcgtgc tgagtctgca catcctcggc gccaccgttt
     7681 gggccggcgg ccatctggtg ctcgcggtga ccatcctgcc cgacgcactc cgccaacacc
     7741 gggccgcgac cgtcagcgag ttcgagcagc ggttcgagcg gatcggtctc ccggcgctcg
     7801 cggtgcagat cgtcaccggc ctgtggctcg cggagcacct cctcgggtca cctgccaact
     7861 ggttcgacgg caactcggtc gcacgaaccg tccaggtcaa gcttggcctc ttggtcatca
     7921 cggtcggact ggccatccat gccaggttcc gcgtcattcc acgactctcc gacgcgaccc
     7981 tgcccacact cgcgtggcac atccgcatcg tcaccctcgc cgcagtcctg ttcgtgctgg
     8041 ctggcgccag catccggttc ggcggggacc ccgtcttcga ccggtgagca cacgcgcacc
     8101 gttcacatca ggttgggtcg agcacgatcc acgccgattg gtagccgccg gatgggtaag
     8161 tcgcccggac ggatgctggg tcgaccactg gtcgaagtcg ttcggtgcca tccagcaacg
     8221 ctgtaacgaa cgattccaac tcgagtcgtg cgagcggggc gccggggcac tcatggatcc
     8281 cggcgccgta cagaaggttc agcgccgggt cgcgatcgag ccggaactcg tcggggtcac
     8341 cgaacacctg ctcgtcccgg ttcgccgatg cccacagcac cgtgatccgc gcctcggagg
     8401 gcagcggcgc gccggcgagg gtgacgggac gtgccgtgct gcgccggttg gcgatcagcg
     8461 gggcgtcgat gcggaggatc tcgtcgatcg ccgccggaat ggccgaccgg tcgacgcaca
     8521 gccgggtcac gacatctggg tggtcggcaa ggtacgcgac gatgatcccg acactcgcgg
     8581 cgatcgtccc caactcgccg acggtccagt tccgcacgat gctgacgatc tcggaatccg
     8641 tgagtggtcg accgtcgacg cgttcgccga gcagtcgggt gctcggatcg tcggcggtgt
     8701 cagcgccggc atctcgtcgc gcgtcgagca ccttgcgcac cacgtcgtcg aagcggcggg
     8761 cgctcgccgc aagtgcgtcg aggtcctcgg cgagcgtggc gcggtgctgc tcgtccaacc
     8821 atgcccgcag cggatggtga agatcgctcg accagcccat gaacgcgcac tgcacgcgaa
     8881 ccgcgaactc gcgaccgagc gtgtccatca cctcgatcgg ccggcctcgc ggggccgagg
     8941 cgaccaggtc ggcggcgatc atgtcggata cgggctcgaa ctgcgccatc ggtcctggcg
     9001 cgaagtacgg ctcgatcagc cggcgatacg acgtgtgatc gggcgggtcc atgccgttgg
     9061 ggacggacag atgtcgggac accagattcc ggtacgtctc gtggtcatgg acgacctcga
     9121 gcacgtcggc gtggcgcagc acggaccacc cgagcagatc actgtgggcg acgggacagc
     9181 ggccgcgcat ctcgtcatag acgctgcgct ggtccgcgag cacgtgaggt gcaagcggat
     9241 cccactcagg gaacgccgac cgttcagatt ggtcgtccat ttcgccacgg gacgctactg
     9301 gcgcatccgg cgcagcgaga gcgccgacga tggtcatctc gatacggtca cggtgtgagt
     9361 gacaacggct ggctgcgaat gatcgaggag aaccccggtc attcgtcgtg gtacatcgaa
     9421 cggttccgga cgatggccgc cgagggtcac gatctcgatg gtgaggcccg gttcgtcgac
     9481 gcaatggtgc cgcgtggggc tcggattctc gacgccggct gtggcccggg acgggtcggc
     9541 gggcggctcg ccgccctcgg tcatcacgtc gtcggcgtcg atatcgaccc cgagttgatc
     9601 gccgcagccc aaggcgatca tccgaatgcg atctggctgg cacgggatct ggccgtgctc
     9661 gaccttgcct ccgagggcgt ctcgaaccgg ttcgatgtga tcgtctcggc gggcaacgtg
     9721 atgacgttcc nnnnnnnnnn nnnnnnnnnn nncccgccgc gacgtccttc accggctgcg
     9781 cacgcatctc gctccggagg gtcgactcgt ggtcgggttc ggcgcagatc gagggtatga
     9841 cttcgacgac ttcttcgccg atgccgcctc ggtggggctc cgcgccgagc tcacgctctc
     9901 gtcgtgggat ctgcgtccct tcgacaccgc atcagcgttc ctcctggccg tcatgaccgc
     9961 cgactgaccc ccgcccggcg acaacgtcga tcctgctgat gccagggtcg atggtcctgg
    10021 tagttcgtca cctgcgactc ggcttgcgtc ggtgccgggg cgaactacgg tcaggttcat
    10081 gatcccgcac cgacacgacg ccgcagcgct cgatcgtcgt ggcgtcgcgt ggggtctggt
    10141 cggggtggcg gtgacgccgg cgatcacgtt cgtcggatcc gacggtctgt tgtggttcga
    10201 cgccgccttg atcggttacc tgttcgggat catcttcatg gtgttcggcg tgatctaccg
    10261 ctacagcgtg tgggtgcggc gtccgtcgac ggcgacgttg aaccggcgcg gctgggatgc
    10321 gttccgtcag cgcaaggccc acaacgccgc ggcgctcccg tcgttggtcg ccacgcagtt
    10381 gttggcgcag ggtttcatcc ggcgcaggtc gcgggcccgc tggttggcgc accagctggt
    10441 gttctggggg tgcatcctcg ccgggttgat cacgttcccg ctcacgatgg gtctgttgca
    10501 cttcgagagc gtgggccagc agggcgaccg ctaccaggtg tacttctcgc gtgtcggcac
    10561 gctgcgcttc gatgccgaca gtgtgatcgg ctggacggtc tatcacgggc tcgacatcgc
    10621 cgcggtgctg gtgctcgccg gcgtgttcat cttcctgcga cgccggctgc gggacccggg
    10681 tgcgctggcc accgaacgaa gcggtgactt cctcgcgctg gccggcctgt tcgcggggtc
    10741 ggtcacgggg ttgttcctga cggtgtcgag catctggctc gagggccgct tctatgcggc
    10801 gctgaacacc ctgcatgcgc tgacggtgat cctcgggttg atgtacatcc cgttcgggaa
    10861 gctgttccac atcttccagc gaccggccaa tctcggcgtc acgtactaca agcgggccaa
    10921 cgccgaaggt ccggccgctg tctgccggcg ttgtggcgac gggttcgcga gtgcgcagca
    10981 gatcgccgac ttgcaggtga tcctgccgca gctcgggttc gactacgccg ccgccgatgg
    11041 cgccgggagc taccaggaca cgtgcccgaa gtgccgccgt gctcagttgg cgttggcaca
    11101 gtcggcacgt gtgggagggt tcggctgatg gcacgtccac cgctcaccga tgccgagctg
    11161 atcgaacgct acggcccaca tctcaaccag gcgccacccg gcggatggaa cgctgggatc
    11221 gaggtggatc gcgtcgtcga cacgcactgc tgcttctgcg gtcagcagtg cggcatcaag
    11281 ttgaaggtgc acgacaacga ggtggtcggg ttcgagccct ggtacgactt cccgttcaac
    11341 gagggcaagc tctgcccgaa gggcgtcaag cggtatctgc agggcagcca cccggaccgc
    11401 gtcgtctatc cgatggaacg cgacgagaac gccccgggcg ggttccggag ggtgtcgtgg
    11461 gaccatgcgc tcgaccgcgt ggtggccgag atccgccgca tccaggacgc gtacggtccg
    11521 gactcgttcg cgatgttgtc gggtgtgtcg ttgacgaacg agaagagcta cctggtcggc
    11581 aaattcgccc gcctcgcgtt gcacacggcg aacctcgact acaacggccg ttactgcatg
    11641 gtgtcggccg gcgctggcaa caagaaggcg ctcggcattg accgagcgtc gaatccgtgg
    11701 agcgacatcc cgcttgccga cgtggtgtgg accgcgggca ccaacatcgc cgaaaccttc
    11761 ccgatcacca cgagctacat ctggaaagcg cgcgaccggg gtgcccggct gatcgtgcaa
    11821 gacccgcgcg tcgtgccaca tgcccgcacc gcggacctgt tcctgcccgt gcgccctggg
    11881 accgactcgg cgttgttcgg ggctgtgttg cacgagctga tccgccacga ctggctcgac
    11941 cacgacttca tcgacgcgca cacgaacgac ttcgaccagg ccgccgccgc ggtcgcggac
    12001 atgactccgg cgtgggcggc cgacatcacc ggcgtcccca aggcccggat cgagcaggcc
    12061 gcggagtggt gggggacgag cgcgaccggg atgctgctgc acgcccgtgg catcgagcag
    12121 cagtccaagg gtgtcgacaa cgtactggcc gcgatcaacc tcgggctcgc cactggcaag
    12181 ttcggcaagc cgggctgcgg tgtcaccacc atcaccgggc agggcaacgg acagggcggt
    12241 cgcgagcacg gccacaagtg cgaccagctc cccggcaatc gcgacatcgc caaccccgaa
    12301 catcgggcgc atgtcgcgtc ggtgtgggga tgcgacgtcg atgagatccc gggcaagggg
    12361 atcccggcac tcgagatcat cgaggcgatc catgccggcg agatcaaggg gctgttgtcg
    12421 atctgcttca acccgttggt gtcgtcaccg gattcgaact tcacccgcga ggcgctcgat
    12481 cggctcnnnn nnnnnngtga tcgacttctt cgtctccgag tccggtcatt acgccgacgt
    12541 catcctgccc ggtttgctcc acgaggagga cgaaggcacc tccacctcgg gcgagggtcg
    12601 gatcatcaag atcaacgcag cggtcgagcc gcccggcgag gcacggcgcg actgggagat
    12661 cctcgtcgac ctcgccgacc gcctcggcag cggccactac ttcccgtaca ccgacacccg
    12721 tcagatcttc gaagagctcc ggctcgcgtc cgccggcggc accgccgact accgaggcgc
    12781 gacgtgggag cggatcgagg ccgagtacgg gctgttctgg ccgatccccg aagaaggaca
    12841 cccgggcacc ccacgcctgt acgaaggcgg ccggttctac caccccgacg gccgagcgaa
    12901 gttccatgcg gtcccgtacc ggctgcccgg cgaggtggtc gacgacgact atccggtgtg
    12961 gctcaccacc gggcgggtcg tcagccagta cctgtccggc acccagaccc gacgcatcgc
    13021 tgcgctcgtc gcccagtacc ccgagccgct gtgtgagatg cacccgcggc ttgcagaaca
    13081 gatcggtgtc gccgacggcg atctggtgac cgtcacgtca cggcggggca cgatcaccct
    13141 gcccgctcac gtggtcgcga cgatccgtcc cgacacggtg ttcatcccgt accactggcc
    13201 cggcgacaag gccgcgaacc agctcaccca acgcgtcgtc gatccgatct cgaagatgcc
    13261 cgagttcaaa gtgtccgccg tgcgggtgga accggcaggc ggtcgggtca cgaccaccga
    13321 tgtccgagac ttcgatctcc agggcggagc aacatgagcg tcgacatccg ctacggcatc
    13381 gacgactcgc cggtgttcgt gatcgaccag agccgctgca tcggctgcga agcgtgcgtg
    13441 caggcgtgca tggaatgcgg cacccaccgc gggcagtcgc tgatccacct cgaacgcatc
    13501 gaccgcgccg cgaccacgca gaccgcgccg atggtgtgca tgcactgcga ggaccccacc
    13561 tgcgcgcagg tgtgccccgc cgatgcgatc aagcagaccg agggcgggat cgtgcagtca
    13621 gcgctcaagc cgcgctgcat cggctgctcc aactgtgtgc tcgcctgccc gttcggcgtc
    13681 ccgaagtaca tggccgagat cgaccagatg atgaagtgcg acatgtgcac cgaccgcacc
    13741 tccgaagggc tcaaaccgat gtgtgcgtcg gtgtgcccga gcgaagcgct ctggtacggc
    13801 accatcgacg agtaccgcga gaaccgcacc gggtcgctgc tgcgcgactt catgttcggc
    13861 cgccaagagg tccgcaccaa ggtctacacc gtcgtcgacg acctcgccgc cggcccgatc
    13921 gacgtgatgc gaggccaagc agcctcgtgg ctcgacgatc cgttcgcact cgaaagcggt
    13981 ggcacatgac agaccgcaga cccgaaccgc agccactccc agtaccgatc tggaagcggg
    14041 acttccccta cgaggccgca gccgaggaag aggtcacccg ccgcgagttc gcccgctacc
    14101 tcgtcgccgg agccggtgcg ctcgcgctgg gcaacgtcgg tctcgccgcg tggacacagc
    14161 tgcgcaccat caacgagggc gaaccccgcg ccatcgtcga cgccgcgagc gtcgaggtcg
    14221 gcggcaccca cctcttccgg tattcgaccg acgccgaccc cgccgtgcta ctccgcatca
    14281 gcgacaccga ggtcgtcgcg ttcagccaga aatgcaccca cctgggatgt gtcgtctact
    14341 acgaagccga cgagcatcgt tggcactgcc catgccacga gggcaacttc gacgccgaga
    14401 ccggcgacgt gatctccgga ccgccattgc gcccactcgg ccgcatcgac gtcgaaacac
    14461 gcgacgacgg catgatctgg gccctcggac ggagccagga aacatgaggg tctccgaacg
    14521 aggccggcgc tcgtcgtcgg ccgtcctgat ctacgtgatc atcctcatgg cgatgcaggt
    14581 cttcctcgtc accgtcgccg ccgaagcgtt cctcgccgac gaaagccggc ctcgcctggg
    14641 ccaccgccat cgtgtccgtc gtgctgttcg ccgccgccgc cagcttcctg cgctacctcc
    14701 gaccgtagaa cgcgatggcc gacaccccgt catcaccgat cgcgacgctg ttccccggct
    14761 acttcgccct cgtgatggcc accggcatcg tcgcgatcgc cgcagaccaa cagaacctcg
    14821 acctgctcgc ccaaggcctc tacgtgatca cggtgctcgc gtacgtcgtg ctcgccgtgc
    14881 tgctcgcctc acgcatgatc cggtactggt cgaacttcgc agctgacgtc accaaacacg
    14941 ccaaaggttt cgcgttcttg accaccgtcg ccgccactaa tgtgctggcc tccgcgtcga
    15001 tcgtcatcca cggctggtgg aacctcgcgt ggtcactgtg gtgggccagc ctcgtgctct
    15061 gggcagcatt cgtctacacc accctcatcg ccgtcgtcac cgaccgcgac aaacccgggc
    15121 tgcagggtgg tatcaacggc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15301 nnnnnnnnnn nnccatccgc tcgacccgac cgaggctgac ccgcccgctt ggatcgccgc
    15361 cggggccgtg gcgatcacgg tgctcgcagg atcgaacatc ctcctcgccg ccgaccaggc
    15421 ccccgaccgg gtcgcccggc tcgccccctt cctcgaagga ctggtcgtga cggcctgggc
    15481 caccgccacg ttctggttcc cgctcatgat cgccatcggc atctggcgtc acctcgtccg
    15541 ccgcgtcccg ctgctctacc acccgagcta ctgggcgatg gtgttccccc tcgggatgta
    15601 cggcgccgcc acctaccgca tgcgagctgc gatcgacctc gacgccctcg cctggctccc
    15661 caagatcgaa ctcgtcgtcg ccctcgctgc gtggaccgtc acgttcgtcg gactcggatg
    15721 gcacctcgcc acgtcccgtc gaacaacacc acacggatag ccaccagatc ccggcgaccg
    15781 ccggcacggc accgatgggg agcagcttca gtgcgtgccg aggccgctgg cgagttggtc
    15841 gaagccgctc tggaccagag ctggcagtgc gggggtgtgc ggcgtggtga gccagtgctg
    15901 cagtgaggcg cgcatgacgg cgagcgccgt cgcggcgagc aggcgtggtg cggggtcgtc
    15961 ggcccgcgcg ccgagtcgtg tggtgagacg ttgggtgagc gcctgttccc aaccggcttg
    16021 gaggtgcagg c
//
LOCUS       DAORNF010000003        15951 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_2802_length_15951_cov_1.868646, whole genome shotgun sequence.
ACCESSION   DAORNF010000003 DAORNF010000000
VERSION     DAORNF010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 15951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15951
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2802_length_15951_cov_1.868646"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="PLV68_00260"
     CDS             <1..255
                     /locus_tag="PLV68_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99697.1"
                     /translation="GHVNNTVYWAIAEEVLAGIGTWREPASVALEHHGAIDAGAAVAV
                     TVAPGPAGADVWVHAGDALAGAKHLAAVIRIRRGPGATAQ"
     assembly_gap    408..427
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <428..1616
                     /locus_tag="PLV68_00265"
     CDS             <428..1616
                     /locus_tag="PLV68_00265"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HRD99698.1"
                     /translation="ADSLTSDDVAREILRAQNQANDAAEAYDTAVRRSESLQVDIAAA
                     QTAVETAQAEYAVVEDGLSRLAVNQYLGGGTNGPLFLSDPLANLQEQALARAAVEAGD
                     VTLNDMSVLHQRLVQRQADLAMLQQQNEQLQASLTRQQSELTTKISQLETLRKRLEDE
                     EVRRAYEAALAEQRRQQEEADRKAEAARQAAAATTVVTLPPAVAPPNAQPTTATTQPA
                     NNPTNAGGNASGGSGGNTSPNTTSPAAPATTSPPVATNAPVITAAPPPVTGSWICPVA
                     GATAFGDSWGNARSGGRTHQGVDMISAAGTPLVAVVGGSVLFKQTPLGGNSVWLTGND
                     GNKYYYAHLSSFEGSSRSVSAGEVIGYVGSTGNASGPHLHFEIHPGGGAAVNPYKTVR
                     QYC"
     gene            complement(1633..2541)
                     /locus_tag="PLV68_00270"
     CDS             complement(1633..2541)
                     /locus_tag="PLV68_00270"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HRD99699.1"
                     /translation="MKFSFWPSNAVAWDELIDLARYTEANGWHAFWFADHFMSQTADD
                     TPGSDPALECWSVLAAIGAAVPRLRLTSMVSPVTIHHPVVLAKRAATIDHISGGRVVL
                     GLGAGWQVNEHAAYGIDLRPVGERVTHFIESIEVIRHLFDDERADFAGRWYTLTDAPF
                     EPKPLQRPLPLLVGTGGDRMMRATARYAQAWNTWGDPAAVAERTERFRAACDSVGTDF
                     DSIHRSAQALVRVTDSDAETERLRAAMPAGRSLVGQPSELIDLLGQYADLGVDEFALP
                     GFTLGATREQRRDNLARFHSDIAAHI"
     gene            complement(2538..3380)
                     /locus_tag="PLV68_00275"
     CDS             complement(2538..3380)
                     /locus_tag="PLV68_00275"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRD99700.1"
                     /translation="MTSASTPTVPSVALGDGKAIPQLGFGVWQVPDDEAETAVLAAFE
                     AGYRHIDTAAIYGNERGVGRAIAKSGLDRDEIYITTKLWNSDHLDAATAYSKSLHKLG
                     LDTVDLYLVHWAAPPLGTYRQAWEGLVSLHQQGLVRSIGVSNFHQPHVDQIVADTGVT
                     PVLNQIELHPYLQQRDMRAYNAGHGIVTEAWSPLGQGKGLLDDPDLAAIASTKGVSPA
                     QVALAWQLAIGNVVIPKSVTPSRIVENLASVDVTLTAEEIEQIEALDRGERFGSDPDT
                     AAFA"
     assembly_gap    3425..3457
                     /estimated_length=33
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3458..3788)
                     /locus_tag="PLV68_00280"
     CDS             complement(<3458..3788)
                     /locus_tag="PLV68_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99701.1"
                     /translation="MALGVGEPEHGTPRRASDDPAVDAEMATDGLDVGDMAVHVDRRP
                     VERGIGRRRRAASRPALVERDRPVQCRVTQIEMPATEPAAWTTVQPHHRHTCRISGLF
                     HMQPMAFT"
     gene            3925..4365
                     /locus_tag="PLV68_00285"
     CDS             3925..4365
                     /locus_tag="PLV68_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99702.1"
                     /translation="MRLVEAYRRLVMDLDPAVHQPAADAWCRWEDAHVDLDDTDPADG
                     FVDPRFADPSFRLAFARQVTHCWAADSWLGPDELVRGAAAMAPVPCWFIHGGRDRSSP
                     LDPVERLHAAWPGSQMVVTDDGHGGAAMWAALRAALRALAVPPT"
     gene            complement(4390..>6159)
                     /locus_tag="PLV68_00290"
     CDS             complement(4390..>6159)
                     /locus_tag="PLV68_00290"
                     /inference="COORDINATES: protein motif:HMM:NF023335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3427 domain-containing protein"
                     /protein_id="HRD99703.1"
                     /translation="NPAGGAMIVALLLGLTATPERSDGLPILQWFDDRIAAELRLWEA
                     IDQQHLSPFLYFGIHDGLDLREVPWRRGRGYDVDALTNAYTSSDAWARLVVKEVAGHT
                     EPSSMRALGFCVSIEHARFMAHHFNLHGIPSVAVWGDSPAADREAALRDLAHGRVRAV
                     FSVDLFNEGVDVPAVDTVLMLRPTESPTLFLQQLGRGLRKSKGKGFCTVLDFVGTHRK
                     EFRFDRRYRALLGGSRRDVEREVQQQFPFLPAGCHMHLDAKAAEVVLRSLRDAIPTRW
                     PAKVDEFRSLRQERPDLGLAEFLVESGLDLDDVYDGNKSWSDLRQAAGADVLAPGPNE
                     VQLRRAIGRLLHIDDRERIDTYLRHLASPVTPELSELPERERRLLHMLLAAVADRALD
                     RDTSVQAAAELLWAHPQVRSELSELFGLLDGRIDHLHTPLATHPDSPLQIHAKYTRIE
                     ILAAFGLGSGAKIAAWQSGVYEAKSANAELLAFTLDKSSGGFSPTTRYRDYAISRSLI
                     HWESQSSTREDSPTGLRYRHHERDGRTILLFSRLSADERAFWFLGPATYRGHVGERPM
                     AVTWQLQQPLSGDLYQAFAAAVA"
     assembly_gap    6160..6203
                     /estimated_length=44
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6204..>6916)
                     /locus_tag="PLV68_00295"
     CDS             complement(<6204..>6916)
                     /locus_tag="PLV68_00295"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HRD99704.1"
                     /translation="TTYTDSTERRALDQLVDLGAEVRISYDLSTTRLHAKAWVFHRHS
                     GFSTAYVGSSNLTHSAQVTGLEWNVRASAARNPDVIAKFGAVFASYWEAGDFVPYDPD
                     QFDDERRRSGRSTSGPHVILSPIELRPHPFQERLLELIELSRQRGHHRNLLVAATGTG
                     KTVMAALDYATLAGRLSRSRLLFIAHRKEILDQSLATFRYALRDPSFGEMWVGGARPK
                     NFDHVFASIQGLNAGDLDA"
     assembly_gap    6917..7111
                     /estimated_length=195
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7366..7384
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7560..8303)
                     /locus_tag="PLV68_00300"
     CDS             complement(7560..8303)
                     /locus_tag="PLV68_00300"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HRD99705.1"
                     /translation="MPGEEVVIRTAEGGCRSWVFTPDDGGGPWPAVIFYMDGLGIRPG
                     LLAMGQRLADAGFVVLLPDLFYRVGPYEPLDPQAVFASGDVRAVIGPLMASTDNHRAA
                     ADTAAFLRYLDGRDDIAGTKVGTTGYCMGGGISLTAAGTHPDRIAAAASFHGGALATD
                     SELSPHRLADQIAARVYIAAADKDRSYPPEMAARLCQALMAASVDHRHELYVGAAHGW
                     TMADFPIYDHTAAERHWDEMIALFDDRLR"
     gene            8431..8670
                     /locus_tag="PLV68_00305"
     CDS             8431..8670
                     /locus_tag="PLV68_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99706.1"
                     /translation="MLHLSANGGGAIVNVLSVLMDTDMTAGVDAPKSDPAHVAAQTLD
                     GVEAGAHEILADGLSRHVRAALSGDLVDLYPVLGR"
     gene            complement(8736..>9193)
                     /locus_tag="PLV68_00310"
     CDS             complement(8736..>9193)
                     /locus_tag="PLV68_00310"
                     /inference="COORDINATES: protein motif:HMM:NF025008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HRD99707.1"
                     /translation="DGMSYSDVPPPAAMPAGTQRIEFPPPAHLNNVETHLHPDTLMLA
                     GADRSEWLAWSRPIDSATFDAGWLTMYGDYFPPAVFARTTAPARAVTIEYSIQIHSAA
                     GAWTLRDDEYLTARLHTFHSHDGFAVEDGWIHLPDGNLLATVRQTRLAG"
     assembly_gap    9194..9225
                     /estimated_length=32
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9226..9534)
                     /locus_tag="PLV68_00315"
     CDS             complement(<9226..9534)
                     /locus_tag="PLV68_00315"
                     /inference="COORDINATES: protein motif:HMM:NF025008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HRD99708.1"
                     /translation="MNWADATALTQLAEHRFGAHIDEQWTSLQGVHGGVVAATVVYAS
                     TAVLRDAGVAPETELRAATFGYVSGNVVGDLTIDIELVRRGRAMVTTHARVIQDGKTT
                     T"
     gene            9612..10082
                     /locus_tag="PLV68_00320"
     CDS             9612..10082
                     /locus_tag="PLV68_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013800920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRD99709.1"
                     /translation="MRRTSFEEMNCSVAQCLEVVGEWWSLLIVRDAFLGVRRFDDFQR
                     RLGIARNVLNERLRTLVDAGVFDRVQYHDRPPRFEYRLTERGRDLWPVITAMRQWGDK
                     WAAPEGPPLQLRHATCGHVANAVTVCSECGEPLDPRAVSAEPGPGATSAAPARG"
     gene            complement(10206..10745)
                     /locus_tag="PLV68_00325"
     CDS             complement(10206..10745)
                     /locus_tag="PLV68_00325"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRD99710.1"
                     /translation="MEALPDAVGTLVDLGCGDGRLIELVLDAHPGLTEAIGLDNSPPM
                     LDLARQRFAGQTRVRIAEHDLAEELPGLHDIDAVVSGFAIHHLPHERKQSLFGEIVAV
                     LRPGGVFINLEVVQCATAELHEEFNRRIERPGGDPQDILAEVEPQLEWMRNAGLTQVD
                     CNWRWRGFALLVGQRPAIF"
     gene            complement(11153..11470)
                     /locus_tag="PLV68_00330"
     CDS             complement(11153..11470)
                     /locus_tag="PLV68_00330"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HRD99711.1"
                     /translation="MSREIELHLAAEAEIEEAGDYLNERTPGEGARFTLAVDAALEAA
                     LRSPRTGQLVELKRSKTEVRRKAVQPHDYQVVYAVYDERILVLAVAHNRRRPLYWRDR
                     VPK"
     gene            complement(11467..11703)
                     /locus_tag="PLV68_00335"
     CDS             complement(11467..11703)
                     /locus_tag="PLV68_00335"
                     /inference="COORDINATES: protein motif:HMM:TIGR02574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HRD99712.1"
                     /translation="MTTATESLLDQVLALPDDERQEFLTRLQERLPELAGDELGDEWI
                     DELDRRLADVESGSVPTVRWEAVKERLLAKYSPE"
     gene            complement(11889..11974)
                     /locus_tag="PLV68_00340"
     tRNA            complement(11889..11974)
                     /locus_tag="PLV68_00340"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11938..11940),aa:Ser,seq:tga)
     gene            12044..12133
                     /locus_tag="PLV68_00345"
     tRNA            12044..12133
                     /locus_tag="PLV68_00345"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12078..12080,aa:Ser,seq:gct)
     gene            12294..13988
                     /locus_tag="PLV68_00350"
     CDS             12294..13988
                     /locus_tag="PLV68_00350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99713.1"
                     /translation="MAVVAAVLIAAAPVLTATLPVSAAAADRIFMIGDSLTYASLPYQ
                     ADELRKAGWSDPVVDGYGARSIRFKIPSDPHTGLDAVTDLRRAHGDADDWIVALGQND
                     SGLYPPTEYPGVIREMLDHIGTGHRVMWVNIYAPADRSRQAAWNAALDQVAAERPDQL
                     VVYDWASVAAGNRSWQLPDKVHYTDFGTRQRSRHVAAAATQVLGISRPSSLGAPAPQP
                     YGPSARLTPTTPLRALDTRAGVRLAAGEIRTVHLADAPEGSVAVAVNVTAVDPSAAGY
                     LTVFPCGQGRPNASMLNYDAGAIAASAVLVAISGNRDLCVFSHAATDVIVDVFGGFTP
                     DGLGYTPVAPARVADTRTARPVATGGVLRVNVGAAAALVNLTVTDGDVDGFVTVYPCD
                     RPLPAVSNVNYLAGEIRSNQAVAVADPSGDVCLYAMSSVHMIVDIVGRFTADGGASFQ
                     AAQVSRLLDTRSGIGGWQGRLAAAGQTVTVTAAGLPGATIVGTLTVVGALGDGFTTMW
                     AGGERPGVSNSNYSTGDIVAASVAAQLAATATATASVFAGPTSPGHVVFDVTGWFA"
     gene            15011..>15515
                     /locus_tag="PLV68_00355"
     CDS             15011..>15515
                     /locus_tag="PLV68_00355"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRD99714.1"
                     /translation="MPATTAAPTTTAPLVCAKDYDVLQGDYWIGIADKVDVRLAELLQ
                     ANRATVKTALYPGRTICLPASASDPTVPTTAAPTTTAKPTQTTPTTSTPTTPTTAKPT
                     TTTAAPATTTTVKPPANTYTKAQVADIIRQIWPDDLEDEAIRIATRESNLIPTVRNSC
                     CFGLFQIY"
     assembly_gap    15516..15685
                     /estimated_length=170
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            15711..15783
                     /locus_tag="PLV68_00360"
     tRNA            15711..15783
                     /locus_tag="PLV68_00360"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15744..15746,aa:Arg,seq:acg)
BASE COUNT         2484 a         5207 c         5260 g         2487 t
ORIGIN      
        1 ggtcacgtca acaacacggt ctattgggcc atcgccgaag aggtgctggc cggcatcggc
       61 acgtggcgcg agcctgcgtc ggtggcgctc gaacaccacg gcgcgatcga tgccggtgcg
      121 gccgtggcgg tgaccgtcgc ccccggcccg gccggcgccg acgtgtgggt ccatgccggt
      181 gatgcgctcg caggggcaaa gcacctggcc gccgtgatcc gcattcggcg cggaccaggg
      241 gccacggcgc agtgagcgcg ttgaccctga tgccgcaact gtcgtccggg caactagcct
      301 acttccgtca tgaaatcgtc atactcgtcg gtggggcgcg tccgagcgcg gttgctcgtc
      361 cgttgtgccg cgctgatcgg ggtggcgtgc gcgatcgtgc cgatggtnnn nnnnnnnnnn
      421 nnnnnnnggc cgactcgctc acctctgacg acgtcgcgcg ggagatcctg cgggcccaga
      481 accaggccaa cgatgccgcc gaggcgtacg acacggctgt gcgccggtcg gagtcgttgc
      541 aggtcgacat cgccgcggcc cagacggccg tcgagacggc gcaggcggag tacgcggtcg
      601 tcgaggatgg gctgagccgg ttggccgtca accagtacct cggcggcgga acgaacgggc
      661 ccctgttcct gtccgatccg ctggccaacc tgcaagagca agcgctggcc cgggctgctg
      721 tcgaagcggg tgatgtcacg ctcaacgaca tgagcgtgct gcaccagcgc ctcgtccagc
      781 gccaggccga cctcgccatg ctccagcagc agaacgagca gctgcaggca tccctcacgc
      841 gtcagcagag cgagctgacc accaagatca gccagttgga gacactccgc aagcggctgg
      901 aagacgaaga ggtccgtcgc gcctacgaag cggcgctggc cgaacagcgt cgccagcagg
      961 aagaggccga tcgcaaggcc gaagcggcgc gccaggccgc agcggcgacc acggtggtga
     1021 ccctgcctcc cgcggtggcc ccgcccaatg cccaaccgac gacggccacc acgcagccgg
     1081 ccaacaaccc gaccaacgca gggggcaacg caagcggagg ttcaggcggc aacaccagtc
     1141 ccaacaccac cagcccggcc gcaccagcca ccacgtcgcc cccggtcgcc accaatgcgc
     1201 cggtgatcac ggccgcaccg cctccggtga ccggttcgtg gatctgcccg gtggcgggcg
     1261 ccacggcgtt cggcgatagc tggggcaatg cccgctccgg ggggcgcacc caccaaggcg
     1321 tcgacatgat cagcgccgcc ggtacgccac tggtcgcggt ggtgggcggg tcggtgttgt
     1381 tcaagcagac accgctcggt ggcaactccg tgtggctcac cggcaacgac ggcaacaagt
     1441 actactacgc ccacctgtcg agcttcgagg gcagttcgcg gtcggtgtcg gccggcgagg
     1501 tcatcggcta cgtcggttcc accggcaacg ccagcggccc gcacctgcac ttcgagatcc
     1561 accccggcgg cggtgcggcg gtcaacccct acaagacggt gcgtcagtac tgctgagtcg
     1621 cgtgtttggc ggtcagatgt gggcggcgat gtcggagtgg aagcgggcga ggttgtcgcg
     1681 ccgctgttca cgcgtggcgc cgagggtgaa gcccggtagc gcgaactcgt cgacgccaag
     1741 atcggcgtac tgaccgagca agtcgatcaa ctcgctcggc tggccgacca acgaccgtcc
     1801 ggctggcatc gcggcgcgca gccgctcggt ctcggcgtcg gagtcggtga cgcgcaccaa
     1861 cgcctgggcc gagcgatgga tcgagtcgaa gtcggtgccg accgagtcgc acgcggcccg
     1921 gaaccgctcg gtccgctcgg cgacggctgc cggatcgccc cacgtgttcc atgcctgtgc
     1981 gtagcgggcg gtggcgcgca tcatccggtc gccaccggtg ccgaccagca agggcagggg
     2041 gcgctgcagc ggcttgggct cgaacggcgc gtcggtgagg gtgtaccagc gaccggcgaa
     2101 gtcggcccgc tcgtcgtcga agaggtggcg gatgacttcg atcgattcga tgaagtgggt
     2161 gacgcgctcg cccaccgggc gtaggtcgat gccgtacgcg gcgtgctcgt tgacctgcca
     2221 gccagcgccg aggccgagca ccacacggcc gccgctgatg tggtcgatcg tcgcagcccg
     2281 cttggcgagc acgacggggt gatggatggt gaccggcgag accatcgatg tgaggcgcaa
     2341 ccgcggcacg gccgcgccga tcgcggcgag cacgctccag cactccaggg cggggtcgct
     2401 gcccggcgtg tcgtcggcgg tttggctcat gaagtggtcg gcgaaccaga aggcgtgcca
     2461 gccgttggct tcggtgtagc gggcgaggtc gatcaactcg tcccacgcca cggcgttgga
     2521 gggccagaac gagaacttca tgcgaacgcc gccgtgtccg ggtcggaacc gaagcgttcg
     2581 ccgcggtcga gtgcctcgat ctgctcgatc tcctcggcgg tcagggtgac atcgaccgag
     2641 gccaggttct cgacgatccg cgacggggtg accgacttgg ggatgaccac gttgccgatc
     2701 gcgagttgcc acgccagcgc gacctgcgcc ggggacacac ccttggtgct ggcgatcgcc
     2761 gcgaggtcgg ggtcgtcgag caagcccttg ccctggccca gcggcgacca ggcctcggtg
     2821 acgatgccgt gcccggcgtt gtaggcccgc atgtcccgct gctgcaggta ggggtgcagt
     2881 tcgatctggt tcagcacggg ggtgacgccg gtgtcggcga cgatctggtc gacgtgcggt
     2941 tgatgaaagt tggagacgcc gatcgagcgc accagtcctt gctgatgcaa cgacaccaga
     3001 ccttcccacg cctggcggta tgtgccgagt gggggagcgg cccagtgcac gaggtacaga
     3061 tcgacggtgt cgagacccag cttgtgcagg ctcttcgaat acgccgtcgc agcatccagg
     3121 tggtcggagt tccacagctt ggtcgtgatg tagatctcgt cccgatccaa accggacttg
     3181 gcgatcgccc ggcccacgcc gcgttcgttg ccgtagatcg cggcggtgtc gatgtgccgg
     3241 tacccggcct cgaacgcagc gagtacggcc gtctccgcct cgtcgtcggg cacctgccac
     3301 acgccgaatc cgagttgcgg gattgccttg ccgtcgccga gtgcaacgga aggaacagtg
     3361 ggggtcgatg cggacgtcac ggccccgacc ctaccgacct cggtggtgtc gctacggtgc
     3421 gctgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncgg tgaaggccat tggttgcatg
     3481 tggaacaatc cggagatcct gcaggtgtgg cggtggtgtg gttgcacggt ggtccaggca
     3541 gcgggctcgg tcgcgggcat ctcgatctgt gtgacccggc actgcaccgg ccggtcgcgt
     3601 tcgaccagcg cgggtcggga cgcagcacgc cgacggcggc cgatcccgcg ctcgactggg
     3661 cgacgatcga catggaccgc catgtcgccg acatcgagac catccgtcgc catctcggca
     3721 tcgaccgctg gatcgtcgct ggcgcgtcgt ggggttccgt gctcgggctc acctacgccc
     3781 agcgccatcc ggagcacacg atcggtgtcg tcgtcgcagc ggtgagcacc ggcggagatc
     3841 gaccatctca ccgagttcgg tcaccgctac gcaccgcggc cctggcagcg gttccacgac
     3901 catgccgcgt cccaccaccc cggcgtgcgt ctcgtcgagg cctaccggcg gctggtgatg
     3961 gacctcgacc cagcggtcca ccaaccggcg gcggacgcgt ggtgccggtg ggaggacgcg
     4021 cacgtcgatc tcgacgacac ggacccggcc gacgggttcg tcgacccgcg cttcgccgat
     4081 ccgtcgttcc ggctggcctt cgcccgccag gtcacccact gctgggcggc ggactcgtgg
     4141 ctcggcccgg acgagttggt gcgtggtgcg gcggcgatgg cgcctgtgcc gtgttggttc
     4201 atccacggcg ggcgcgatcg cagcagcccg ctcgatccgg tcgagcggct ccacgccgcg
     4261 tggcccggga gccagatggt ggtcaccgac gacggccacg gcggggccgc gatgtgggcc
     4321 gctctacgcg cagccctgcg cgccctcgcg gtccccccga cctgagccgc acgcgacgcg
     4381 ctcggttgat caggccaccg cggcggcgaa ggcttggtac agatcgccgg agagcggctg
     4441 ctgcagctgc caggtgacgg ccatcggacg ctcgcccacg tggccgcgat aggtggcggg
     4501 gcccaggaac cagaaggcgc gctcgtcagc actgagccgg ctgaacaaca ggatcgtgcg
     4561 gccatcccgc tcgtgatgtc ggtagcgcag acccgtgggg ctgtcctccc gcgtggatga
     4621 ttggctctcc cagtggatga ggcttcggct gatcgcgtag tcgcggtacc gggtggtcgg
     4681 cgagaatccc ccgctgctct tgtcgagcgt gaacgcgagc aactcggcgt tggccgactt
     4741 cgcctcgtac acgccgctct gccacgccgc gatcttggcg cccgaaccca gcccgaatgc
     4801 ggcaaggatc tcgatccggg tgtacttcgc gtggatctgc agggggctgt cggggtgcgt
     4861 ggccagcggt gtgtggaggt ggtcgatccg accgtcgagc aagccgaaca actccgacag
     4921 ttcgctgcgg acttgtggat gggcccacaa cagttcggcg gctgcttgaa cgctggtgtc
     4981 gcgatccaac gcacgatcgg caacggcggc gagcagcatg tgcagcagac gccgctctcg
     5041 ctcgggcagc tctgacagct cgggggtcac gggcgatgcc aggtggcgca ggtatgtgtc
     5101 gatgcgctcc cgatcgtcga tgtggaggag gcgtccgatg gctcggcgca gctgcacttc
     5161 gttcgggcct ggcgcgagca cgtcggcgcc ggcggcctgt cgaagatccg accaactctt
     5221 gttgccgtcg tagacgtcat cgaggtcgag cccggattcg accaagaact cggcgagccc
     5281 gaggtcgggc cgctcttgtc gaagcgaacg gaactcgtcc accttggccg gccagcgagt
     5341 ggggatcgcg tcgcgaaggc tgcggaggac gacctctgca gccttcgcgt cgaggtgcat
     5401 gtgacagccc gccgggagga aggggaactg ctgctgcact tcccgctcga cgtcgcggcg
     5461 cgagccgccg agcagggccc ggtagcggcg gtcgaaacgg aattccttgc ggtgcgtgcc
     5521 gacgaagtcg agaacagtac agaagccctt gcccttcgat ttgcgaaggc cacggccgag
     5581 ttgttgcagg aagagcgtcg ggctctcggt cggacggagc atcagcaccg tgtcgacggc
     5641 cgggacgtcg acaccctcgt tgaacaggtc gacggagaac actgcccgaa ctcgcccgtg
     5701 cgcgaggtcc cgcagcgccg cctcgcggtc ggccgcagga ctgtcgcccc agacggccac
     5761 cgaagggatg ccgtggaggt tgaagtggtg ggccatgaac cgggcgtgct cgatgctgac
     5821 gcagaacccg agcgcgcgca tcgaagacgg ctccgtgtgg ccggcgactt ccttcaccac
     5881 cagccgagcc cacgcgtcag aactcgtgta cgcgttggtc aaggcgtcga cgtcgtagcc
     5941 gcgtccccgg cgccacggga cctcgcgcag gtcgagcccg tcgtgaatgc cgaagtacag
     6001 gaagggcgac aggtgctgct ggtcgatcgc ctcccacagt cgaagttcag cggcgatgcg
     6061 atcgtcgaac cactggagaa tcggcaggcc gtcgctgcgc tctggcgtcg cggtcagacc
     6121 gaggagcagc gcgacgatca tcgcgccgcc cgccgggttn nnnnnnnnnn nnnnnnnnnn
     6181 nnnnnnnnnn nnnnnnnnnn nnncgcatcg agatcaccgg cattgaggcc ttgtatcgat
     6241 gcgaagacgt gatcgaagtt cttcggtcgt gcaccgccga cccacatctc tccgaacgac
     6301 gggtcgcgca gcgcgtatcg gaacgtggcc aggctctggt cgaggatctc cttgcggtgg
     6361 gcgatgaaca gcagtcgaga tcggctgagc cgtccagcga gtgttgcgta gtcgagtgcc
     6421 gccatgacgg tcttgcccgt gccagtggcg gcaacgagga ggttgcggtg atggcctcgc
     6481 tggcgagaga gttcgatgag ttcgagcagg cgttcctgga acgggtgtgg ccgcaactcg
     6541 atcgggctga ggatcacgtg tgggccgctg gtgctgcggc cggaccgccg ccgctcatcg
     6601 tcgaactggt cggggtcgta cggaacgaag tcgccggcct cccagtagct ggcgaacaca
     6661 gcgccgaact tggcgatgac gtccgggttg cgcgcagccg acgctcgaac gttccactcc
     6721 agcccggtca cttgcgccga gtgggtgaga ttcgatgaac cgacataggc agtcgagaag
     6781 ccggagtggc gatggaacac ccacgccttc gcgtggaggc gtgtggtgct caggtcatag
     6841 gagatccgca cctctgcacc gagatcgacg agctggtcga gtgcccggcg ctccgtcgag
     6901 tcggtgtagg tggtcgnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngcccggcga tggcggccgg cgagccatca
     7141 gggttgcggc ggaggatggc atggaggact gaggccgggt cggcgagcct cgcttgctgg
     7201 tcaacgtcga cgagctcacc gaggcgatcg agcaatgcac gtgccactcg aagcccgacg
     7261 ttcacgcgat cttcgtcgcc gacgtcgagc agtgcccgct cgacgtgctg gctcacatgc
     7321 caggcaatcc gatccgaggc gtcagcgctg ttcagcggcc gctcgnnnnn nnnnnnnnnn
     7381 nnnngctcga cgatgggcct gttatgcgcc agttcgtcga gctgcgacct cagccgctgc
     7441 gtgatgagct gctcgtagat cccaggagtc agctccgcca cactgcgacg ttacgacaag
     7501 ccagccttgc cgtcgccgaa ctctgggact tctttgccgt cacttccgct acgggcagat
     7561 cagcgcaagc ggtcgtcgaa caacgcgatc atctcgtccc agtgccgctc ggcagcggtg
     7621 tggtcgtaga tgggaaagtc ggccatcgtc cagccgtgtg ctgcgccgac gtacagctcg
     7681 tggcgatggt cgacgctcgc cgccatcagc gcttgacaca gtcgggcagc catctccggc
     7741 gggtacgagc ggtccttgtc ggcggcggcg atgtagaccc gagctgcgat ctgatcggcc
     7801 agccgatgcg ggctgagctc cgagtcggtg gcgagcgccc cgccgtggaa gctggccgcg
     7861 gcggcgatcc tgtccggatg ggtgccggca gccgtcagcg agatgccgcc gcccatgcag
     7921 tagccggtgg tgccgacctt ggttccggcg atgtcgtcgc gaccgtcgag gtagcgcaag
     7981 aacgcagcgg tgtcggctgc cgcccggtgg ttgtcggtcg atgccatgag cggaccgatc
     8041 accgcccgga catcaccgga ggcgaacacg gcctgtgggt cgagcggctc gtatggcccg
     8101 acgcggtaga acaagtcggg caacagcacc acgaagccgg cgtccgcgag gcgttgtccc
     8161 atcgcgagca gcccgggccg gatgccgagt ccgtccatgt agaagatcac cgctggccac
     8221 ggtcccccgc cgtcgtccgg ggtgaacacc cacgagcggc agccgccctc ggcagtacgg
     8281 atcacgacct cttcacccgg catggttcac ccctgtgatc ttgttctgcg ctgcccaccc
     8341 atcgagcacg cccaccaatc agcctcgcgc atcaacgcgc cggactgtcg gtgccatcgc
     8401 ccgaggagac tgccgagtgg gtcgtcagag atgttgcacc tgtcagccaa cggcggcggg
     8461 gcgatcgtca acgtcttgtc cgtgctcatg gacaccgaca tgaccgccgg cgtggatgcc
     8521 cccaagtccg atcctgcaca cgtcgcggcg cagaccctcg acggcgtcga ggccggcgcc
     8581 cacgagattc tcgccgacgg cctcagccgc cacgtccgag ccgcgctctc cggcgatctc
     8641 gtcgacctgt acccagtgct ggggcgttga cccgaacgct gcggtcgaca tcatcgccgc
     8701 ccaacggtca gcgcggctgc gccgaccgtg tcaggtcagc cggccaggcg ggtctggcgc
     8761 actgtcgcga ggaggttgcc gtcggggagg tggatccatc cgtcttcgac cgcgaagccg
     8821 tcgtgggaat ggaacgtgtg cagccgcgcg gtgaggtact cgtcgtcgcg cagggtccat
     8881 gctcctgccg cactgtggat ctggatcgag tactcgatgg tcacggcgcg tgcgggtgcc
     8941 gtcgtgcggg cgaacaccgc tggtgggaag tagtcgccgt acatggtgag ccacccggcg
     9001 tcgaaggtgg cgctgtcgat cggtctgctc caggccagcc attcagagcg gtctgcgccc
     9061 gcgagcatca aggtgtcggg gtgcaggtgg gtttcgacgt tgttgagatg tgccgggggc
     9121 gggaactcga tccgttgggt cccggcgggc atcgcggccg gtggtggcac atcgctgtaa
     9181 ctcatgccgt cccnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncgtgg tggtctttcc
     9241 atcttggatg acacgggcgt gcgtggtgac catcgccctg ccgcggcgca cgagctcgat
     9301 gtcgatcgtc aggtcaccca ccacgttgcc gctgacgtag ccaaaggtcg ctgcgcgcag
     9361 ttcggtctcg ggcgcgacac cggcatcgcg cagcactgcg gtggaggcat acaccacggt
     9421 cgcggcgacc acaccgccgt ggacgccctg caacgaggtc cactgctcat cgatgtgcgc
     9481 gccgaagcgg tgttcagcga gttgggtcaa agcggtggca tcggcccagt tcacgatgtc
     9541 gacccgtcaa cgttcaggtg gtgtgccacg tggatggacg gtagccggtt gcaacctact
     9601 acggtgtggg tgtgcgtcga acgagcttcg aggagatgaa ctgctcggtc gctcagtgcc
     9661 tcgaggtggt gggtgagtgg tggagcctgt tgatcgtgcg cgacgcgttc ttgggtgtgc
     9721 gtcgcttcga tgacttccag cgccggctcg ggatcgcccg taacgtgctc aacgagcggc
     9781 tgcgcacgct ggtcgatgcc ggggtcttcg accgcgtcca gtaccacgac cggccgccac
     9841 gcttcgagta ccgcttgacc gaacggggtc gcgacctgtg gccggtgatc accgcgatgc
     9901 gtcaatgggg cgacaagtgg gcggcaccgg agggcccacc gctgcaactg cgacacgcca
     9961 cttgcggcca cgtcgcgaat gcggtcaccg tctgttccga atgtggcgaa ccgctcgatc
    10021 cacgcgccgt cagcgccgag cccggaccgg gcgccacatc agcggcaccc gcccgcggtt
    10081 gatccctctc ggacgtctgg cgctcgacgc cggttgaaat cgacggagaa gaactgggtc
    10141 cgctgatact cgtctgccga ccacggcacc gacccagatc gccgggtttg aggcggccgt
    10201 tctcgctaga agatcgccgg cctctgaccc acgagcagag cgaaacctct ccagcgccag
    10261 ttgcagtcca cctgtgtcaa cccggcattg cgcatccact ccagttgcgg ctccacctcg
    10321 gccaggatgt cctgcggatc gccaccaggg cgctcgatcc tgcggttgaa ttcctcatga
    10381 agctcagcgg tcgcacactg cacgacctcg agattgatga acacgcctcc cggccgcagc
    10441 accgcgacga tctcaccgaa cagtgactgc ttccgctcat gaggcaggtg atgaatcgcg
    10501 aagcccgaga ccactgcgtc gatgtcgtgt agaccgggaa gctcctcggc aagatcgtgt
    10561 tcggcgattc tgacccgcgt ctgccctgcg aaccgctgcc gggcgaggtc gagcatcggt
    10621 ggtgagttgt cgaggccgat cgcctcggtc agtccggggt gggcgtccag cacgagttca
    10681 atcagacgcc catcgccaca tccgagatcc acgagcgtgc ccacggcgtc ggggagcgct
    10741 tccaacagct ccgcctcgcc cgcctgtcga gcgaccaacc gaccgacgcg gccggtgtac
    10801 tcggcaacct tgtcctggtc tttccaactc gacgccacga tcacagcatg gccgctcggc
    10861 aaccccgcca ctcacacaag aagtcgtcga tcaatgcttg ggggcgggcg ccgccgagtg
    10921 gtgtgccgtg caccaacgca acggcgaggt cggcgtccaa tatttcatcg accaaggctg
    10981 acacgtcgac gcgttgggtc ctttgcgacc caccgaaaac gcatcggcgg aacgggagtg
    11041 attccgccag ctcagcgtag gagccgcgaa gtcccaggtc agaaacggtg tgaggtgctg
    11101 agccgggagt aaccccggga gtaacgcagc ggcccaatcc cgaacgcggc tgctacttgg
    11161 gaacccgatc gcgccagtag agcggcctgc ggcgattgtg cgcgactgcc aacacgagga
    11221 tgcgctcgtc gtacaccgcg tacacgacct ggtagtcgtg cggctgcacg gccttgcggc
    11281 ggacctcggt cttgctgcgt ttgagctcga ccaactgccc ggttcgtggg ctcctcagcg
    11341 cggcttccaa cgcggcatct acggccaatg tgaaccgggc accttcgcct ggcgtgcgct
    11401 cgttgaggta gtcgccggcc tcctcgatct cggcctccgc agcgaggtgg agttcgatct
    11461 cccggctcac tcgggggagt acttggcgag gagtcgttcc ttgactgctt cccacctgac
    11521 ggtcggcacg ctgccggact cgacgtctgc caatcgacga tccaactcgt cgatccactc
    11581 atcgccgagt tcgtcgccag ccaactccgg cagccgctcc tgcagtcgcg tcaggaactc
    11641 ttggcgttcg tcgtcgggca acgcgagcac ctgatccagc agtgactcgg tagcggtggt
    11701 catgagtgaa cgatacctcc aggcgctgca gccgtcagct tggaagtagg ggagtctccg
    11761 gtctgtggac aagcccagat tccgatgtca caactggcct tctcggccgg cccattgacg
    11821 cacatcggtg gacgtttcac ctggtcatag cgcattcggt ggacgctctg acacgtcggt
    11881 ggacctgttg gcggaacgag agggattcga acccccgggg cgtgaaccct tccgctttca
    11941 aggcggatgc atttggccgc tctgccatcg ttccggcgac gacgctagcg ccacccgagt
    12001 tctctacggc cgttccggat gatcgccagc taacatggcg aacggagagc tgccagagcg
    12061 gccgaatggg gcaccctgct aaggtgttgg tggtttcggc cacccgtggg ttcaaatccc
    12121 acgctctccg ccaagtgttc gttgtcgatc tcagcccagc agtgagacca gaacgtgacc
    12181 gaccgaagac ccgggccagc caggctcggg tctcttcgtt gcccgaccct ggcggtgatg
    12241 tcggtggccg tcggcgtggt gccggacggc atgcccgtcc gatcctgcgt cggttggcgg
    12301 tcgtcgcagc ggtactgatc gctgctgccc cggtgctcac cgcgacgctg ccggtgtcgg
    12361 ccgccgctgc cgacaggatc ttcatgatcg gcgactcgct cacgtacgca tcgcttccct
    12421 atcaggcgga cgagttgcgc aaggccgggt ggtccgaccc ggtcgtcgac ggctacgggg
    12481 cacggtcgat ccggttcaag atcccctccg acccgcacac cgggctcgat gcggtcaccg
    12541 atctgcgccg cgcccacggc gacgccgacg actggatcgt cgcgctgggc cagaacgact
    12601 ccggcctcta cccgcccacc gagtacccgg gcgtgatccg cgagatgctc gaccacatcg
    12661 gtaccgggca tcgggtgatg tgggtgaaca tctacgcacc ggccgaccgt tcccgccaag
    12721 ccgcatggaa cgccgcgctc gaccaggtcg ccgccgaacg tcccgatcag ctcgtcgtct
    12781 acgactgggc ttcggtcgcc gcaggcaacc ggtcgtggca gctccccgac aaggtgcact
    12841 acaccgactt cgggacccgg caacgttcca ggcacgtcgc cgcggccgcc acccaagtgc
    12901 tcggcatctc ccgcccatcc agcctcgggg cgcctgcccc gcaaccgtac ggaccgtccg
    12961 cccggctcac cccgaccacc ccgctccgtg cactcgacac ccgcgcaggc gtccgtctcg
    13021 ccgccggcga gatcaggacc gtacatctcg ccgacgcgcc ggagggcagc gtcgctgtcg
    13081 cggtcaacgt caccgcggtc gacccgtccg ccgccggcta tctcacggtg ttcccgtgcg
    13141 gtcaagggcg accgaacgcg tcgatgctca actacgacgc cggtgcgatc gcagcgtcgg
    13201 ccgtgctcgt cgccatctcc ggcaaccgtg atctgtgcgt cttctcgcat gccgctaccg
    13261 acgtgatcgt cgacgtgttc ggtggattca cacccgacgg gctcggctac accccggttg
    13321 cccccgcgcg cgtcgctgac acccgcacgg cccgaccggt ggctacaggt ggggtgctgc
    13381 gcgtgaacgt cggtgccgcg gcagcgctcg tgaacctgac cgtcaccgac ggcgacgtcg
    13441 acggcttcgt caccgtctac ccgtgcgacc ggccactgcc ggcggtctcg aacgtcaact
    13501 acctcgctgg ggagatccgt tcgaaccagg ccgttgcggt cgctgatccg agcggggacg
    13561 tgtgcctgta cgcgatgtcg tcggtgcaca tgatcgtcga catcgtcggc cggtttaccg
    13621 ccgatggtgg tgcttcgttt caggcagcgc aggtttcccg gctgctggac acccgctcgg
    13681 ggatcggtgg ctggcaggga cggctcgccg ccgccggaca gacggtgacg gtgaccgccg
    13741 ctggtctgcc cggcgcgacg atcgtcggca cgctgaccgt cgtcggggct ctcggcgatg
    13801 ggttcacgac catgtgggcc ggaggcgaac gtccgggcgt gtcgaactcg aactactcca
    13861 ccggcgacat cgtcgctgcg tcggtcgcgg cgcagctcgc cgcgaccgcg accgcgaccg
    13921 cgtcggtgtt tgccggcccc acctcgccag gccacgtcgt gttcgacgtg accggctggt
    13981 tcgcgtgacg tgagcggtcg cgcatagcac gcgccatagc agatgtcagg tcggccatcg
    14041 cggaccctat tgttgcaatc tcatgacggt gcgtgtagac atttcgtaac gcgacgggca
    14101 gccggtccgc cgtgacgacg acacagccca ccgtgcgcgg cgggcagggg cgagtaccgc
    14161 ccctccaacg cctccatccg accagtcggg aggaggacgt gaagggacag cacatgcctt
    14221 acccggactc acaccgccca gatgaaccca gagcgcgcgc cgacgccgag tcgtgggacc
    14281 ccgccttctg gagcaactcg aacgagcaga tcggtcaaca gcaagcaggc aggaagcggg
    14341 ccgggcgaga tgtcactaga ccactgcgtc gtgtgggctg gtctcgccag tcgtcacccg
    14401 acgcgtcatc ggtgccccag gatcagaccc gatatacgca cggcagtgtc gatgcgaact
    14461 actcgcgctc ccagtggctc gacgggcatg acgacggtat cgacgcgctc tgggccaacc
    14521 cgcgcagccg cgtctcgcgt ccgccgcgcc gtggtggtgt tcccgcggcc cccaacttcg
    14581 cggcgcgacg cgtcggtgcg ttggtgctcg tcggtctgct ggcggcgccg gtcgcgtggc
    14641 tggccagcgg tagggacgac gagcgcgtcg ccgacagcgc cgacgaagca ctgatcgccg
    14701 atgccatgcc ggtgtcgcca atcgctgcgg ctgacggcgt cgcatcgtcg ggcgaacaga
    14761 cgacggctgc cacctgccgg cgtccgcctt cgcggcggcg ccgaactcgg ctgccaccca
    14821 caccggggcg tcggcaccgg ccactgctgc atcagcggcc accaccgcgg tgacgacggt
    14881 cgtttcgcca gcagcaacgg ctgcctcagc cgaatcgtca gccacgaccg agggacggac
    14941 gagcacaggc gccacccctc acgcagagaa ggccgagcaa gccgagacga cggcagtgcc
    15001 gacgacggcg gtgcccgcga cgactgccgc gcccacgacc acggccccgc tggtgtgcgc
    15061 gaaggactac gacgtcctcc agggcgacta ctggatcggt atcgccgaca aggtcgacgt
    15121 gcgccttgcc gagttgctcc aggccaaccg ggccacggtc aagacagcgc tgtatccggg
    15181 ccgcacgatc tgcctgccgg cgtcggcgtc cgatccgacg gtgccaacga cggcggcacc
    15241 caccacgaca gccaagccga cgcagaccac gcccacgacg tcaacgccaa ccactccgac
    15301 aacggccaaa ccgaccacga ccaccgctgc gcccgcgacg accaccacgg tcaagccacc
    15361 ggccaacacc tacaccaagg ctcaggtggc cgacatcatc cgccagatct ggcccgacga
    15421 cctggaagac gaggcaatcc gcatcgcgac ccgggagagc aacctgatcc cgacggtgcg
    15481 caactcctgc tgcttcggcc tgttccagat ttactnnnnn nnnnnnnnnn nnnnnnnnnn
    15541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15661 nnnnnnnnnn nnnnnnnnnn nnnnngcccc tctaggatgg cgtcgcttct gcgctcgtag
    15721 ctcaatggat agagcacttg actacggatc aagcggttag gggttcgagt cccttcgagc
    15781 gcgcagagca gacgagcggc cttcgggccg ctcgtctgcg ttctggctcc agccacgctc
    15841 gatcaatcgg atgcaccggc aaggcgcatg tggcggatgc gccagatggc accggcgagc
    15901 atgagcaccg tgaccacgac aaggcggacc accgcgccgc cgccgtcggg g
//
LOCUS       DAORNF010000004        14539 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_3140_length_14539_cov_1.976871, whole genome shotgun sequence.
ACCESSION   DAORNF010000004 DAORNF010000000
VERSION     DAORNF010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 14539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14539
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3140_length_14539_cov_1.976871"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>311
                     /locus_tag="PLV68_00365"
     CDS             <1..>311
                     /locus_tag="PLV68_00365"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRD99715.1"
                     /translation="YSSRLLADLGATVVKVEAPGGDPLRGSVRMFRAAQANKQSLALD
                     LRRSESTEIVDRLIRWSDVVHHNMRPRVALELGLTAERVQAVNPAAIFGMASGWASNG
                     P"
     assembly_gap    312..401
                     /estimated_length=90
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <402..793
                     /locus_tag="PLV68_00370"
     CDS             <402..793
                     /locus_tag="PLV68_00370"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRD99716.1"
                     /translation="NSDMGNGIIGAIAILIGLLHREITGRALSYENPHLNASMVHVAH
                     IVRTSTGDLLGSERLDPMQYGFSAFDRLYQTADGWVCVAAHTARERLGLARFAAQECD
                     DAPGSDDAASEDAGDEERIADQVGRRC"
     gene            812..1150
                     /locus_tag="PLV68_00375"
     CDS             812..1150
                     /locus_tag="PLV68_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99717.1"
                     /translation="MLADLAALGVPAVAPVGYHNTTFMRDPEQRRTGRVAQVWSPTDG
                     WVRELATLVRVSDAGAVAHRLAPAIGDATRAVLTILGYADGDIDRLCASGVVSEPSVN
                     DDIANARSKQ"
     gene            1147..2037
                     /locus_tag="PLV68_00380"
     CDS             1147..2037
                     /locus_tag="PLV68_00380"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRD99718.1"
                     /translation="MNGLNRPSAGLDLGPGSVAVVTGGGSGIGAALARRLVRDGVSVV
                     IADVAPDLAAVATEIGAVAIAADVSDQASVEALAEQTFDRFGRVDLLCNNAGIGRPRS
                     VLDETLDGWRRMIDVNVFGVVHGLRAFLPRLLAADHPSHVLNTSSIAGIHRNARWIAY
                     STTKFAIVGISEALTDQLAGTQVGISVLCPGPVDTPMGPLQHPDAVRPPDAAPGTAAG
                     MRILSADEVAEQAIAGVSAGQFWIITDGYVESELTARLAAQRAGMQRGRGRRQYWRLL
                     TSRNSSKPKVPPSRPRPDCL"
     gene            complement(1956..3377)
                     /locus_tag="PLV68_00385"
     CDS             complement(1956..3377)
                     /locus_tag="PLV68_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRD99719.1"
                     /translation="MHDWTKQYIDGQWVESHEARTIDVINPTTEQVIARVSAGHADDV
                     DAAVRAAARAFPAWSATTPEERADFLRRLIAAVKERHEVFAQLITAEVGTPIRTARVI
                     QAGLGVVDLEGALEALENFEWREPLRTSVIVREPIGVVGAITPWNYPLHQITAKIGAA
                     IGAGCTVVLKPSEVAPGCAFELTSIIHDLGLPAGVFNVVCGEGQTVGEPLVSHELVDM
                     VTFTGSNKVGKRISELAAATVKRVALELGGKSASLIAPDADLASAVAQTVRGCYINTG
                     QSCSALSRMLVQRDQLAEVEQLTVQAVAGYQPGDPADDQTKLGPIVSAAQQERVLGFI
                     RGAIDEGAQLLTGGLDRPESAPDTGYFVAPTVFTGVDASMTIANEEVFGPVMVIMPYD
                     TEEEAVELANATKYGLAAAVWAGTPERAEHLALQMRAAQVSVNGGQFNSVAPFGGYKQ
                     SGLGREGGTFGFEEFLEVKSLQY"
     gene            complement(3419..>3919)
                     /locus_tag="PLV68_00390"
     CDS             complement(3419..>3919)
                     /locus_tag="PLV68_00390"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HRD99720.1"
                     /translation="ERAVAAGADGLVLLTAGAGGQTGWMNPFAFVRAVRAWFDGPIVL
                     AGGIADGASVWAALALGCDLAYIGTGFIPTREGLASAAYRQAVLAASLDDVELFMQDT
                     GIPASMLRTAATPPPAERDPLTPPSDFSAGHSVSLTNAEVTVAELVARLRAEFGTARA
                     ASLGAA"
     assembly_gap    3920..3929
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3930..4340)
                     /locus_tag="PLV68_00395"
     CDS             complement(<3930..4340)
                     /locus_tag="PLV68_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99721.1"
                     /translation="MGNAWTDWNRRLRLPVIAAPMTGVSGPDLVIEACVNGVIGSFPT
                     QNTPTLHDVDEWLQRIDAGIAARAGELAIEPAPYAVNLVMRRDGARLAAEVDALVRHG
                     VELVITSVGSPAPVLADLHQAGVTVLADVATLHHA"
     gene            4519..>5540
                     /locus_tag="PLV68_00400"
     CDS             4519..>5540
                     /locus_tag="PLV68_00400"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRD99722.1"
                     /translation="MAGFDFLQGVTVVEVAQLGPSALGGYLADMGADVIKVEGAQGDP
                     IRRSGAEGAGSPDGYGYQHLRWNRGKRSVVIDLKSARGIELFRDLVAGADVVYEGMRA
                     GVLERLGVGYDVLRAEHPSLVFCSLSGYGLTGPYSAMGSSGPSYDAFGGMGAFSPYSH
                     DHDAAEFESRVIGMHAVSLYAAMGVIAAIAKARLTGVGSLVEVSAAEAAAHWTPASLD
                     RVLNEGVNHQRPGFNGESGKMLGWPLLNEYTASDGRRIFFQGRYPRFWGKFCELVDRA
                     DLLAIADAGDDDRLFAELTELFASRTAAAWMEFFLVNDIAGSPVNTLRDLAVDPHFVA
                     RDNVYEV"
     assembly_gap    5541..5641
                     /estimated_length=101
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5822..6982
                     /locus_tag="PLV68_00405"
     CDS             5822..6982
                     /locus_tag="PLV68_00405"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRD99723.1"
                     /translation="MQFGFGGKQMDSELFDEDHENFRGIVREFVRQEVEPNLRRWDSE
                     RRIDRATWRAAARQGILGLPIPEQFGGGGATDYRFRVVVQYELARVGANALQSGFSTN
                     DDIVVHYLLRQATPEQAARWLPGFADGTSIGAIAMSEPAAGSDLRGISTRAERTANGW
                     RLNGSKTFITSGIEADLVVVFARTDDSFTLFAVERGMAGFERGRQLDKVGLHAQDTAE
                     LFFTDVELSDQQVLGVVGGGLAALKQSLPLERLGIAIAAQASAEAVLGWTISYVKERR
                     AFGARLADLQVIGHRLAHLHTAVEVSREYVHRCIREWNAGALTAIDAAKAKAWATDLQ
                     GEVIDAGVQFHGGYGYMVEYPVARAYLDARIQRIYGGANEVMMEIVQRDLLR"
     gene            complement(7031..8707)
                     /locus_tag="PLV68_00410"
     CDS             complement(7031..8707)
                     /locus_tag="PLV68_00410"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRD99724.1"
                     /translation="MSHELVIKGGTVVDGTGAPAYTADIAVNGGTITAIGHDLGTGTR
                     TVDADGLVVAPGWVDIHTHLDGQAIWDPEMTPSSWHGVTTAVMGNCGVGFAPMHLDQR
                     ELLVEIMEEVEEIPAEALLSGIDWSWETFGEYLDALDSVGRTVDVAALIPHVALRVYV
                     MGSRAHDEATPDDIAALAKAAEQALREGAAGLSTSRTVMHSSRHGYVPGTSATLDELF
                     ALGGALQRAGHGVMQMNSDFKGGPSDRSWTTDFVDRLGVPIVYILSQTRKAPGAYLEA
                     LDYAAQDVAAGRAIVPQVSCRPAGVLFGLSGSRHPFITHPTYRAMEHLPVSEQVARLR
                     DPETRRQLLSEHRDDNDPRIRELIGAGLWDLWHNMFPLGEKPDYEPEAASSIAAIAGT
                     RNPAEVALDLMLEQDGQALLFSPMSNYTDGNHDAILTQMTHPGTVLGLSDAGAHLRSI
                     CDASSTTYMLTHWVKGRTRGARLSTGQAAQRFGLADRGVLATGKRADINLIDMDAIEL
                     LPPTAVADLPGGGSRLIQKANGYVGTFVAGEQTYSDGEATGVRPGRVCRF"
     assembly_gap    8777..8858
                     /estimated_length=82
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8859..10413)
                     /locus_tag="PLV68_00415"
     CDS             complement(<8859..10413)
                     /locus_tag="PLV68_00415"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRD99725.1"
                     /translation="MVAACGSDSDSGGSPTTTTASSSTTTATSGGNDGGSTTAAPSSS
                     VSEDNIDRSAHANVTYWGSPASLDPHMEQNGGQRVYWRVAYDTLFEENPAGGGIPGLA
                     ASFDRSDDGLEYTLHLRTDVQFADGTPVDAAAAKASLDRVKADGVPGVAGLLNAVESV
                     TVTDGETVVIKLTEPTPELPAILSGPAGAVINPKALADPEALISQTAGSGPYQSTKFV
                     PETEVSYERSSVEYWQPDAGRLGSLTITFVSDVQTAVNGLLAGQFDAMHTVGQLEQIK
                     QAEAANKGIIYDYASTSSEVVFLSATRGVLADQRVRQAMAFAVDRDGIAEGVYQGDCA
                     STSQPFLEGSALHVPTEPYNYDPDKARELIKEAGAEGASIELVNQATSVHNAASAALQ
                     QMWEAIGLDVTIRPEPSASAAFTGFAGGEQDTFMAGFGRQADPMLAVSRYYLAGGVFG
                     LAADDTELQDLVDQVRDPATPAADLAGLNLQIAERVSAGAYALLTCTPTNHITAQPNV
                     VGLDGMRNQA"
     assembly_gap    10956..10977
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10978..11997
                     /locus_tag="PLV68_00420"
     CDS             <10978..11997
                     /locus_tag="PLV68_00420"
                     /inference="COORDINATES: protein motif:HMM:NF013044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HRD99726.1"
                     /translation="EIAMMKRLPVVVAHHSELADNGSFLTRDILGVPLLIVRQSDGSI
                     AAFYNMCRHRGGKVECADAGAKRVFMCQYHGWTFDRDGGTLRNIPFSESFGDIDKECY
                     GLLKVRAEERYGLIWVDFSENADTPIADYVGPEVDAQLASFGLDDSVLFLDEELEVDV
                     NWKLVMDGANDILHLKFLHPNGVGRLITTGTSAFERFGRHGQQFSPRKRMETLAKEGG
                     EVEDIWKYIGSNLRLFPNSMCILSPDHIEFWTVWPDLHSPAKCTIKLRFLVRPEILND
                     EMAERVTRSWEILKQAALEEDFPMEMTIQANSNAVPDGHFVYGRSELSIQQFHEQLQA
                     EIVGA"
     gene            12059..>12257
                     /locus_tag="PLV68_00425"
     CDS             12059..>12257
                     /locus_tag="PLV68_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HRD99727.1"
                     /translation="MHVDLLVVNGTVIDGSGLPRFRGDVAIKGEEVVAVGLLHGVTAD
                     RIIDAAGKVVAPGFIDPQTHLD"
     assembly_gap    12258..12273
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            12318..>13410
                     /locus_tag="PLV68_00430"
     CDS             12318..>13410
                     /locus_tag="PLV68_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99728.1"
                     /translation="MLTGNCSVSLAPCKTEDRNALARLFHKLEDIPIEAFTEGIDWRW
                     ESFGELADALDPTLAINVAAMVGHSTLRFAVMGPESQEREATDEEVQAMCALLLQSLE
                     GGAIGLSTDRLIQHVGDGGRPIPSRMASDEELFALCEAMRDRGTGLLQSGPATNVPDT
                     PAYVRDVLRPIALNTGMTVLLSGTVQERGAPDRWREIHDLIAEGQRDGARIFTQATPC
                     RIDGRFSLAHTLQFNEMPTWRHVLSLPLDERKAAFRDPEVRDRLQFEAVEDTRPIFFS
                     RRWDTIFVLEIESEKNQHLIGQNVLEASQRDGKRVVDWVLDLVLEEDLQCAFVAIGRA
                     NGDDDAVVAQLVSPQAIVGSSDAVAHVMQM"
     assembly_gap    13411..13493
                     /estimated_length=83
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <13494..13770
                     /locus_tag="PLV68_00435"
     CDS             <13494..13770
                     /locus_tag="PLV68_00435"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRD99729.1"
                     /translation="GLTFKPASVLGLSRRGLLRKGYAADVVVFDPDAIEYLDTRMVDD
                     LPSGASRLWRDSVGIDYVIVNGQVAIDADGYTGSRSGSFLRGREFAG"
     gene            13767..>14539
                     /locus_tag="PLV68_00440"
     CDS             13767..>14539
                     /locus_tag="PLV68_00440"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRD99730.1"
                     /translation="MTSAPESDQAPGPNPGRSTSTFAAFHHRNFAGFFVGQLISQVGH
                     WLQAVAQVLLVIHLTDNGEALGWLAMCQFAPVLLLGAWAGALADRSDKRRLLLWIQLG
                     TMVQSVCMVLVAFAERPSLAAIYLLATIRGVSIALDSPARRSFIAELVPEQDVSNAVS
                     LYVAMMTGARVVGPALAGILVTAVGFGWTFFADAASFLAVILSLVAMNRARLRPSPRM
                     ARTRGQVRESARYARGIRDLWIPIVMSAIIGTFAWNFQTV"
BASE COUNT         2249 a         4562 c         4870 g         2454 t
ORIGIN      
        1 catattcgtc acggctgctg gccgacttgg gggccacggt cgtcaaggtg gaagcaccgg
       61 gcggtgaccc gctgcgtgga tcggtacgga tgtttcgcgc ggctcaggcc aacaagcagt
      121 cgctcgctct cgacctgcgc cgcagtgagt cgaccgagat cgtcgaccgt ctgatccgct
      181 ggtccgacgt ggtccatcac aacatgcgtc cgcgggtcgc gctcgagctc ggcctcactg
      241 ccgagcgggt ccaggcggtg aaccctgccg ccatcttcgg catggcgagc ggatgggcgt
      301 cgaacgggcc gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngcaactccg acatgggcaa
      421 cggcatcatc ggagcgatcg cgatcttgat cggtctgctt catcgcgaga tcacaggccg
      481 ggcgttgagc tatgagaacc cccacctcaa cgcgagcatg gtccacgtcg cccacatcgt
      541 gcgcaccagc acgggagatc tgctcggaag cgagcggctc gacccgatgc agtacggctt
      601 ttccgccttc gatcggctgt accagacggc tgacggctgg gtgtgcgttg ctgcccatac
      661 ggcacgtgaa cgactcggac tggcccgctt cgcggcgcag gagtgcgacg atgcacccgg
      721 atcggatgat gccgcatctg aagatgccgg tgacgaggag cgaatcgccg atcaggtcgg
      781 gcggcggtgt tgagccagtg cgacggacca ggtgctcgcc gacctggcgg cgctcggggt
      841 acccgccgtc gcgccggtcg gctatcacaa cacgacgttc atgcgcgatc ccgagcagcg
      901 ccgtaccggg cgggtggcac aggtgtggag tccgaccgat ggctgggtac gcgagctcgc
      961 cacactggtg cgggtctccg acgcaggggc ggttgcgcat cggcttgcgc cagcgatcgg
     1021 tgacgccacc cgggccgtgc tcaccatcct tggttacgcg gatggcgaca tcgaccgcct
     1081 gtgtgcgtcc ggcgtcgtca gcgagccgag cgtgaatgac gacatcgcga acgcgaggag
     1141 caaacaatga acggactcaa ccgacccagc gctggactcg atctcggccc cggcagcgtc
     1201 gcggtggtga ctggcggcgg ctcgggcatc ggagccgcgc tggcccggcg gctcgtgcgc
     1261 gacggagtat cggtggtgat tgccgacgtc gcgccggatc tcgccgccgt cgcgaccgaa
     1321 atcggagcgg tcgcgatcgc tgctgacgtg tccgaccaag cgagcgtcga agcgctggcc
     1381 gagcagacct tcgaccgctt tgggcgggtg gacctgctct gcaacaacgc cgggatcggt
     1441 cgcccacgct ctgtcctgga cgagacgctc gacggctggc gccggatgat cgacgtcaac
     1501 gtgttcggtg tggtccacgg cctgcgggcc ttcctgcccc gcctgctggc tgcggaccac
     1561 ccgagccacg tgctcaacac ctcgtcgatc gcgggcatcc atcgcaacgc gcgctggatc
     1621 gcctattcca ccaccaagtt cgcgatcgtc ggtatcagcg aggcgctgac cgatcaactc
     1681 gccgggacac aggtcggtat cagcgtgctg tgccccgggc cggtcgacac gccgatgggg
     1741 ccactgcaac atcccgacgc ggtccgcccg cccgacgcgg cgcccggtac cgcggcaggg
     1801 atgcgcatcc tgagcgccga cgaggttgcc gagcaggcga tcgctggtgt gagcgctggg
     1861 cagttctgga tcatcaccga cgggtacgtc gagtcggagc tcaccgctcg tctcgcggcc
     1921 caacgcgcgg gcatgcagcg tggacgcggg cgccgtcagt actggaggct cttgacttcg
     1981 aggaactcct cgaagccgaa ggtgccgccc tcgcggccga ggcccgactg cttgtagcca
     2041 ccgaaaggtg cgaccgagtt gaactgaccg ccgttgaccg acacctgcgc ggcgcgcatt
     2101 tgcagcgcca gatgctcggc ccgctcgggc gtgcctgccc acaccgcagc ggcaaggccg
     2161 tacttggtcg cgttggcgag ctcgacggcc tcctcctccg tgtcgtaggg catgatcacc
     2221 attacgggcc cgaagacctc ttcgttggcg atcgtcatgc tcgcatccac gccggtgaac
     2281 acggttggtg ccacgaagta gccggtgtcg ggagccgact ccggccggtc gagaccaccg
     2341 gtgagcaact gcgcgccctc gtcgatcgcg ccgcggatga agccgagcac ccgctcctgc
     2401 tgagcggcgc tgacgatcgg gccgagcttg gtctgatcgt cggccgggtc gcccggctgg
     2461 tatccggcaa cggcctgcac ggtgagctgc tcgacctcgg cgagctggtc gcgctggacc
     2521 agcatgcgac tgagcgccga gcaactctgc ccggtgttga tgtagcagcc gcgtacggtc
     2581 tgtgcgaccg cggacgccag gtcggcgtcg ggtgcgatca gcgatgcgct cttgccgccg
     2641 agttcgagcg cgacacgctt gacggtcgcc gcggccaact cggagatccg cttgccgacc
     2701 ttgttcgatc cggtgaaggt caccatgtcg accagctcgt gcgacaccag cggctcaccg
     2761 acggtctggc cttcgccaca gacgacgttg aacacaccgg ctggcaatcc gaggtcgtga
     2821 atgatcgagg tcagctcgaa cgcgcagccg ggcgcgacct cgctcggctt gagcacgacg
     2881 gtgcaaccgg caccgatcgc agccccgatc ttggcggtga tctggtgcag cggatagttc
     2941 cacggcgtga tcgcgccgac cacaccgatc ggctcgcgca cgatgaccga ggtacgcagc
     3001 ggctcgcgcc actcgaagtt ctccagcgcc tccagcgcac cttcgaggtc gaccacgcca
     3061 agcccggcct ggatcactcg ggcggtgcga atcggtgtgc cgacctcggc ggtgatgagc
     3121 tgagcgaaca cttcatggcg ttccttgacg gcggcgatca ggcggcgcag gaagtcggcc
     3181 cgctcctcgg gggtggtcgc cgaccatgcc ggaaaggccc gcgctgcggc gcgcacggcg
     3241 gcgtccacgt cgtcggcgtg gcccgcgctg accctggcga tgacctgctc ggtcgtcggg
     3301 ttgatgacgt cgatcgtgcg ggcttcgtgg gactcgaccc actggccgtc gatgtattgc
     3361 ttggtccagt cgtgcattgg ttgctcctcg tacagatggg gtgtcgcgag atggggtgtt
     3421 aggccgcgcc cagcgatgcg gcgcgggcgg tgccgaactc ggctcgcagg cgggcgacga
     3481 gttcggccac ggtgacctcg gcgttggtga gtgagacgga gtgaccggcc gagaagtcgg
     3541 acggtggcgt gagtgggtcg cgctcggcgg gcggcggggt ggccgcggtg cgcagcatgc
     3601 tggcggggat gccggtgtcc tgcatgaaca actcgacgtc atccaggctc gccgcgagca
     3661 cggcttggcg atatgccgcg ctggccaggc cttcgcgggt gggaatgaag ccggtgccga
     3721 tgtaggcgag atcacaaccg agggcgagcg cagcccacac cgaggcgccg tcggcgatcc
     3781 cgccggcgag cacgatcggc ccgtcgaacc atgcccgcac ggcgcgcacg aaggcaaacg
     3841 ggttcatcca cccggtttga ccaccggctc cggcggtgag cagcaccagg ccatccgcac
     3901 ctgccgcgac cgcccgctcn nnnnnnnnng gcgtggtgca gcgtggcgac gtcggcgagc
     3961 accgtcacgc ccgcctggtg caggtcggcc agcaccggtg ccggcgagcc gacgctggtg
     4021 atgacgagtt cgaccccgtg gcgcaccagc gcgtcgactt cggccgccag gcgagcgccg
     4081 tcgcggcgca tcaccaggtt caccgcgtag ggagcaggct cgatggccag ttcgccggct
     4141 cgggcagcga tgccagcgtc gatgcgctga agccactcgt cgacgtcgtg caacgtcgga
     4201 gtgttctggg tggggaagct gccgatgacc ccgttgacgc atgcttcgat cacgagatcg
     4261 gggccagaca ccccggtcat cggtgccgcg atcacgggca gccgaagccg tcggttccag
     4321 tcggtccacg cgttccccac accaacatac taaacgaagt ttggtatatc tccaaggccg
     4381 aaagggcttc caggtaaggc gcacggcatc tggtgcgccg gtgtcagtct cacgtgggcg
     4441 aacggtgttg gtattctcgc gacgatgctg gggtcgccag cacgatcgaa ctcgggatcg
     4501 agttgagggg gtacgacgat ggctggattc gacttcttgc agggcgtcac cgtggtcgag
     4561 gttgctcagc tcggcccgtc cgcgcttggc ggctatctgg ccgacatggg cgccgacgtc
     4621 atcaaggtcg agggcgcgca gggcgatccg attcgccgct cgggagcgga gggcgccggg
     4681 tcacccgacg ggtacggcta tcagcacctg cgttggaacc gcggcaagcg cagcgtcgtg
     4741 atcgacctca agtcggcgcg cggcatcgag ttgttccgtg acctggtcgc tggcgcggac
     4801 gtggtctacg aaggcatgcg cgccggcgtg ctggaacggc tcggagtggg ctacgacgtg
     4861 ctccgcgccg agcacccgag cctggtgttc tgttcgctct cggggtacgg gctaaccggg
     4921 ccgtacagcg cgatgggatc gtccggcccg tcgtacgacg cgttcggcgg catgggggcg
     4981 ttcagcccgt acagccatga ccacgacgcg gccgagttcg agtcgcgtgt catcgggatg
     5041 cacgccgtca gcctgtatgc ggcgatgggc gtgatcgccg caatcgccaa ggcgcgtctc
     5101 accggggttg gatcactggt cgaggtctcg gctgccgagg ccgcggcgca ctggacgccc
     5161 gcctcgctcg atcgggtgct caacgagggc gtcaaccatc agcggcctgg gttcaacggc
     5221 gagagcggca agatgctcgg gtggccgctg ctcaacgagt acaccgccag cgacgggcgg
     5281 cgcatcttct tccagggtcg ctatccccgg ttctggggca agttctgcga gctcgtcgac
     5341 cgtgccgacc tgctcgcgat cgccgatgcg ggtgacgacg accggctctt cgcggaactg
     5401 accgagctgt tcgcgtcacg caccgcggcg gcgtggatgg agttcttcct tgtcaacgac
     5461 attgccggca gcccggtcaa cacgttgcgc gacctcgctg tcgacccgca cttcgtcgcc
     5521 cgcgacaacg tgtacgaggt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5641 ncgcggcgat cgccgagcgg ctcgggctgt ctggcgcaga actggccgaa cttcgtgagc
     5701 agcgagtgat cgcctgatcg tctcacccgt ggccgtctca ctcgtggccg tcgccccgtg
     5761 gtggtcgcgc cgtggtggtc gcgccgtggt tgttggtgga cggtgagcgg cccataatca
     5821 aatgcagttt ggttttggag ggaaacaaat ggacagtgag ttgttcgacg aggatcacga
     5881 gaacttccgc ggcatcgtgc gtgagttcgt acgccaggag gtcgagccga acctccgtcg
     5941 ctgggattcc gaacgcagga tcgaccgggc gacctggcgg gcggccgctc gccagggcat
     6001 cctcgggttg ccgatccccg agcagttcgg cggtggcggc gcgaccgact atcggttccg
     6061 ggtcgtcgtg cagtacgaac tggcgcgggt tggggccaat gcgttgcagt ccgggttttc
     6121 gaccaacgac gacatcgtcg ttcactatct gctccgccag gccaccccgg agcaagcggc
     6181 tcgatggctg ccaggtttcg ccgacggaac ctcgatcggt gcgatcgcca tgtctgaacc
     6241 cgccgcgggg agtgacctgc gcggtatctc gacgcgcgcc gagcgcacgg cgaacggatg
     6301 gaggctgaat ggaagcaaga ccttcatcac atcgggcatc gaggcggacc tcgtggtcgt
     6361 gttcgccagg acggacgact cgttcacact cttcgcggtg gagcgaggta tggccggttt
     6421 cgagcgcggt cgtcaactcg acaaggtcgg tctgcacgct caggacacag ccgagctctt
     6481 cttcaccgac gtcgaactgt cagatcagca ggtcctcggc gttgtcggcg gcgggctcgc
     6541 ggcgttgaag cagagcctcc cgctcgagcg gctcggcatc gcaatcgcgg cgcaggcctc
     6601 ggctgaggcc gtgctgggtt ggaccatcag ctacgtcaag gaacggcggg cgttcggtgc
     6661 acgcctcgcc gatctccagg tcatcggcca ccgcctcgcc cacttgcaca cggcggtcga
     6721 ggtgtctcgc gagtacgtcc atcgctgtat ccgggagtgg aacgctggag cactcacggc
     6781 gatcgacgcg gccaaggcca aggcgtgggc gaccgacctt cagggtgaag tgatcgacgc
     6841 cggtgtccag ttccacggcg ggtacggata catggtcgag tacccggttg cccgcgccta
     6901 tctcgacgct cgtatccagc gaatctacgg tggcgcgaac gaggtgatga tggagatcgt
     6961 gcagcgcgac ctccttcgct gaggccgcgg cgggtcctcg ctcgaccggg tcgagcgagg
     7021 accggaccac tcagaaccgg cagaccctgc caggccgtac ccctgtcgcc tcgccatcgc
     7081 tgtaggtctg ctcgcctgcg acgaaggtgc ccacgtagcc gttggccttt tggatgagac
     7141 ggctgccgcc gccaggcagg tcggcgaccg cagtcggtgg caacagttcg atcgcgtcca
     7201 tgtcgatcag gttgatgtcg gctcgcttgc cggtggcgag cacaccgcgg tcggccaacc
     7261 cgaagcgctg tgcggcctgg ccggtcgaca gacgggcccc gcgggtgcgg cccttgaccc
     7321 agtgggtgag catgtacgtc gtcgagctgg cgtcgcagat cgagcggagg tgagcgccgg
     7381 cgtcggacag tccgaggacg gtacccgggt gggtcatctg cgtcaggatc gcgtcgtggt
     7441 tgccgtcggt gtagttggac atgggcgaga acagcagcgc ctgaccgtcc tgttccagca
     7501 tcaagtcgag ggcgacctcg gcagggttgc gcgtccccgc gatggcggcg atgctgctcg
     7561 ccgcctcagg ctcgtaatcg ggcttttcgc cgagcgggaa catgttgtgc cacaggtccc
     7621 acagaccggc gccgatcagc tcgcggatgc gcgggtcgtt gtcgtcgcgg tgctcactga
     7681 ggagctgtcg acgagtctcg gggtcgcgca ggcgggcgac ctgttcggac acggggaggt
     7741 gctccatcgc tcggtaggtg ggatgggtga tgaagggatg acgtgagccg gacagaccga
     7801 acaacacacc ggctggacgg cagctcacct ggggcacgat tgcccgcccg gcggcgacgt
     7861 cttgtgcggc atagtcgagc gcttccaggt aggcgccggg cgccttgcgg gtctggctga
     7921 ggatgtacac gatcggcacg cccaggcggt ccacgaagtc ggtcgtccag gagcgatcgg
     7981 acgggccgcc cttgaagtcg ctgttcatct gcatcacgcc gtgtccggcg cgttgcagcg
     8041 ccccaccgag cgcgaacagc tcgtcgagtg tggccgaggt gcccggcacg tagccgtgac
     8101 gcgacgagtg catcacggtg cggctggtgc tcagcccggc tgcaccctcg cgcagcgcct
     8161 gctcggccgc cttggccagc gcggcgatgt cgtccggtgt ggcctcgtcg tgggcccgcg
     8221 agcccatcac gtacacccgc aatgccacgt gcgggatcaa tgccgcgacg tcgaccgtgc
     8281 gcccgaccga gtcgagcgca tcgagatatt cgccgaaggt ctcccacgac cagtcgatgc
     8341 cgctgagcaa cgcctcggcc gggatctctt cgacctcttc catgatctcg accagcagtt
     8401 cgcgctggtc gaggtgcatc ggtgcgaacc cgacgccaca gttgcccatc accgccgtgg
     8461 tgacgccgtg ccaactggat ggcgtcattt cgggatccca gatggcctgt ccgtcgagat
     8521 gggtgtggat gtcgacccag ccgggcgcga cgacgagtcc atccgcgtcc acggtgcgag
     8581 tgcccgtgcc caggtcgtgg ccgatcgcgg tgatcgtgcc cccgttcacg gcgatgtcgg
     8641 cggtgtacgc cggggcaccg gtgccgtcga ccacggtgcc gcctttgatg accagttcat
     8701 gagacatgtg cgtgtactcc acttgagacg aggccgggtg gcatcgcgtt cagacgatgc
     8761 cacccggcgg ctgcggnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnca gcttggttgc gcatgccgtc
     8881 caacccaacg acgttcggct gcgctgtgat gtggttggtc ggggtgcagg tcagaagcgc
     8941 ataggcgcct gcgctcaccc gctctgcgat ctgcaggttc agaccggcca aatcggcggc
     9001 tggcgtggca gggtcgcgca cctggtcgac caggtcctgc aactcggtgt cgtcggccgc
     9061 gagaccgaac acaccaccgg cgaggtagta gcggctgacg gccagcatcg ggtcggcttg
     9121 acgaccgaag cccgccatga acgtgtcctg ctcgccgcct gcgaagccgg tgaaggccgc
     9181 gctcgcgctg ggctcagggc ggatcgtcac gtcgaggccg atcgcttccc acatctgctg
     9241 cagcgcagcc gaggccgcat tgtgcacgct ggtggcctgg ttgaccaact cgatgctcgc
     9301 gccttcggcc ccggcctcct tgatcaactc acgtgccttg tcggggtcgt agttgtaggg
     9361 ctcggtcggc acgtggagcg cgcttccctc caagaacggc tggctcgtgg aggcgcagtc
     9421 gccctggtac acgccctcgg cgatgccgtc gcggtcgacg gcgaaggcca ttgcctggcg
     9481 cacacgctgg tcagccaaca cgccgcgagt cgccgacagg aagaccacct cggatgaggt
     9541 cgacgcgtag tcgtagatga tgcccttgtt ggcggcctcg gcctgcttga tctgctcgag
     9601 ttgacccacg gtgtgcatcg cgtcgaactg accggcgagc aagccgttca cggcggtctg
     9661 cacgtccgag acgaaggtga tcgtcagact gccgagccga ccggcgtcgg gctgccagta
     9721 ctcgaccgac gagcgctcgt agctgacctc ggtctcgggc acgaacttcg tcgactggta
     9781 cgggccggag ccagcggtct ggctgatcag tgcctcgggg tcggcgagtg ccttcgggtt
     9841 gatcacggcg ccagcgggac ccgacaggat cgcgggcagt tcgggcgtcg gctcggtgag
     9901 cttgatgacc acggtctcgc cgtcggttac ggtgaccgac tcgacagcgt tgagcagccc
     9961 cgccacaccc ggcacgccgt cggccttgac gcggtcgagg ctggccttgg cggcagcggc
    10021 gtcgacgggg gtgccgtcgg cgaactgcac gtctgtacgc aggtggagcg tgtactccag
    10081 accatcgtca gaacggtcga acgacgcggc gaggcccggg atgccgcctc ccgccggatt
    10141 ctcttcgaac agcgtgtcat aggcgacacg ccagtagacg cgctggccgc cgttctgctc
    10201 catgtgggga tcgagcgatg ccggcgaacc ccagtacgtc acgttggcgt gtgcgctgcg
    10261 gtcgatgttg tcctcgctga cgctcgaaga cggcgccgcg gtggtgctgc caccgtcgtt
    10321 gccgccgctg gtcgcggtcg tggtgctcga actcgcagtg gtcgtggtgg gtgacccacc
    10381 gctgtccgag tccgatccgc acgcagcgac catcagtccc actgctgcga gcccagctgc
    10441 gaatttcagt cggcgcgttg ccgtgcctct cttcaacatt tccccctcct tgatagcggt
    10501 accgtctgcc gttgtggcaa cggtgggtgg tttggaggag agctcgtcgc tggatcgacc
    10561 gcctggctca ccgagttggt cgagcgcatg cagattcgat cggcgttcgc cccctcgatg
    10621 cgagcgtatg ggccgctcgt tgcattactt gcctgtccaa cctatcacag attggttaac
    10681 ttggtcaagt gggtcgccag cgccggtagt gcatcagaca ccttgcgcac ctgcctcggg
    10741 ctctctataa tgcaacattg gttggttaga gcgagccgag cgcccgcgcc ggagatgcgc
    10801 tggcggcgat cgcagagaaa aggggacctc aggtatggcg agtgtcaagg acaccacagt
    10861 gagcaaggcc gtcgcacagc gccttctgga gcacttgcgc aatgacacga ccgacatggc
    10921 cccggacgtg ctcgtggtgc cggtcgagca cttcannnnn nnnnnnnnnn nnnnnnngag
    10981 atcgcgatga tgaagcggct gccggtcgtg gtcgctcacc actccgaact ggccgacaac
    11041 ggctcgttcc tcacgcgcga catccttggc gtgccgttgc tgatcgtgcg tcagagcgac
    11101 ggcagcatcg ccgccttcta caacatgtgt cgccatcgcg gcggcaaggt cgagtgtgct
    11161 gacgccggag ccaagcgtgt cttcatgtgc cagtaccacg gctggacctt cgaccgcgac
    11221 ggtggcacgc tgcgcaacat cccgttcagc gagtcgttcg gcgacatcga caaggagtgc
    11281 tacggcctgc tcaaggtccg tgccgaggaa cgctacgggc tgatctgggt cgacttctcc
    11341 gagaacgcag acacgccgat cgcggactac gtcggcccag aggtcgacgc acagttggcg
    11401 agcttcgggc tggacgacag cgttctgttc ctcgacgagg agctcgaagt cgacgtcaac
    11461 tggaagctgg tgatggatgg cgcgaacgac attctccacc tcaagttcct ccacccgaac
    11521 ggtgttgggc gactgatcac cactggcacg agcgcgttcg agcgcttcgg ccgccacggc
    11581 cagcagttca gcccgcgcaa gcggatggag acgttggcca aagagggcgg cgaggtcgag
    11641 gacatctgga agtacatcgg gtcgaacctg cgcctcttcc cgaactcgat gtgcatcttg
    11701 tcgcccgacc acatcgagtt ctggaccgtg tggcccgatc tccactcgcc ggccaagtgc
    11761 acgatcaagc tgcgcttcct ggtcaggccc gagatcctca atgacgagat ggccgagcgg
    11821 gtcacgcgca gctgggagat cctcaagcag gcagcactcg aagaggactt ccccatggaa
    11881 atgacgatcc aggccaactc gaacgccgtg ccggatggtc atttcgtcta cggccgcagc
    11941 gaactgtcga tccaacagtt ccacgaacaa cttcaggcgg agatcgtcgg ggcctgactg
    12001 gagtggatca cctgtggtga tccacccgtc gtcatcattg atcaccaaag gggaatacat
    12061 gcacgtcgac ctactcgtcg tcaacgggac cgtcatcgac ggttcgggac tgccgcgctt
    12121 tcgcggtgat gtcgcgatca agggtgagga ggtggtcgca gtcggattgc tgcacggagt
    12181 caccgccgat cggatcatcg acgccgccgg caaggtggtg gcacccggat tcatcgaccc
    12241 ccaaacccac ctcgaccnnn nnnnnnnnnn nnngggatcc gacggggtcg ccgtcactcc
    12301 aacatggtgt gacgacggtg ttgacgggca actgcagcgt cagcctcgcc ccgtgcaaga
    12361 ccgaggaccg caacgccctg gcacgcctgt tccacaagct cgaagacatc cccatcgagg
    12421 ctttcaccga gggtatcgac tggcggtggg agtcgttcgg ggagctggcg gatgcgctcg
    12481 acccgacctt ggcgatcaac gtcgccgcga tggtgggaca ttccacgctg cgctttgccg
    12541 tgatggggcc ggagtcgcaa gagcgcgagg caaccgacga ggaagtgcag gcgatgtgcg
    12601 ccctgctgct gcagagtctg gaaggcggcg cgatcgggct gtcgacggac cgcctgatcc
    12661 aacacgtcgg tgatggtggc cgacccatcc cgagccggat ggcgagcgac gaagaactgt
    12721 tcgcgctgtg cgaggcgatg cgcgatcgcg gtacggggtt gctccagtcg gggccagcga
    12781 ccaacgtccc tgacacaccc gcgtacgtac gtgacgtact ccgccccatc gcactgaaca
    12841 ccgggatgac cgtgttgctc agcggcaccg tgcaagagcg cggtgcacct gaccgctggc
    12901 gagagatcca cgacctgatc gccgaaggcc agcgcgacgg tgcacggatc ttcacccagg
    12961 ccacgccgtg tcgcatcgac ggacgcttct cgttggcgca cacgctccag ttcaacgaga
    13021 tgccgacgtg gcgccacgtg ctgtcgttgc cgctcgacga acgcaaagcc gcgttccgcg
    13081 accctgaggt gcgcgaccgg ctgcagttcg aagcagtcga agatacgcga ccgatcttct
    13141 tctcacggcg ctgggacacg attttcgtgc tcgagatcga gtcggagaag aaccaacacc
    13201 tgatcggcca gaacgtgctc gaagcctctc agcgcgacgg caagcgggtg gtcgactggg
    13261 tgctcgacct ggtgctcgaa gaagacctgc agtgcgcgtt cgtcgcgatc gggcgggcca
    13321 atggcgatga cgacgccgtg gtcgcgcaac tggtcagccc ccaagccatc gtcggctcca
    13381 gcgacgcggt tgctcacgtg atgcagatgt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncgggttg
    13501 acgttcaagc cggcgtcggt gctcggcctc tcccggaggg gcctgctgcg caaggggtac
    13561 gcagccgatg tcgtcgtgtt cgacccggac gcgatcgagt acctcgacac gcgcatggtc
    13621 gacgacttgc ccagcggtgc ttcgcggctg tggcgtgaca gcgtcggtat cgactacgtg
    13681 atcgtcaacg gccaggtggc catcgacgcc gatggataca cggggagccg gtcgggcagc
    13741 ttcttgcgcg gccgcgagtt cgccggttga caagcgcgcc ggagtcggat caggcgcctg
    13801 gaccgaaccc gggccgatcg acatccacct tcgccgcgtt tcaccatcga aatttcgccg
    13861 ggttcttcgt cggccaactg atctcccagg ttgggcactg gctgcaagcc gtcgcccagg
    13921 tgctcctggt catccatctc accgacaacg gcgaggccct cggttggttg gcgatgtgcc
    13981 agttcgcgcc ggtcttgctg ttgggcgcgt gggccggggc gctcgccgat cgctccgaca
    14041 agcggcggct gttgctgtgg attcagttgg gcacgatggt gcagtccgta tgcatggtgc
    14101 tggtcgcgtt cgccgaacgc ccctcgctgg cagctatcta tctcctggcc acgattcgag
    14161 gcgtgtcgat cgctctcgac agcccggccc ggcgttcgtt catcgccgag ttggtgcccg
    14221 agcaggacgt gtccaacgcg gtcagcctct acgtggcgat gatgaccggt gcgcgcgtgg
    14281 tcggcccggc gctggccggg atcctcgtca ccgcggtcgg cttcggctgg acgttcttcg
    14341 ccgatgcggc gtcgttcttg gcggtcatcc tgagcctcgt cgcgatgaac cgggcgcggt
    14401 tgcgtccatc gccgcgcatg gctcgcaccc ggggccaggt gcgcgagtcg gcccgctatg
    14461 cgcgcggcat tcgcgacctg tggattccga tcgtgatgtc ggccatcatc gggacgttcg
    14521 cgtggaactt ccaaacggt
//
LOCUS       DAORNF010000005        13160 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_3574_length_13160_cov_1.695078, whole genome shotgun sequence.
ACCESSION   DAORNF010000005 DAORNF010000000
VERSION     DAORNF010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 13160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13160
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3574_length_13160_cov_1.695078"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="PLV68_00445"
     CDS             <1..373
                     /locus_tag="PLV68_00445"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRD99731.1"
                     /translation="SDVKAASVALRDLRSLGLHLSVDDFGTGYSSLTYLKRFPVEAIK
                     VDRTFVKGLGIDAEDTTIVEAVVNLGRSLGLRVVAEGVETPLQLSRLREMGCAYAQGY
                     LFGRPRPAALIEAERAGSFAP"
     assembly_gap    559..634
                     /estimated_length=76
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<635..832)
                     /locus_tag="PLV68_00450"
     CDS             complement(<635..832)
                     /locus_tag="PLV68_00450"
                     /inference="COORDINATES: protein motif:HMM:NF036456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5318 family protein"
                     /protein_id="HRD99732.1"
                     /translation="MSFSVDAFRSGRPSAGQHSRRAVVDHRLERLALVNEYRRGRLRR
                     EQICDAHPELIRAAKNVGTQSN"
     gene            906..1502
                     /locus_tag="PLV68_00455"
     CDS             906..1502
                     /locus_tag="PLV68_00455"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRD99733.1"
                     /translation="MAVVRYGTSIADDAELRLIGDVTGKRVLELGISTPSNAVVLAAN
                     GARALPADPSADRIAALRREAEAAEVTVQCHQVAVAELGPIASASIDVVVAAHSLDEV
                     DDLARVLRQVHRILKPDASFVVAVRHPIDAIVTSADGNTIVTIPYGAGGVRSIGDWFM
                     SMQRTNFAIDVVHELRSIGAAHSLTPTTLVLRAKKLGV"
     assembly_gap    1570..1712
                     /estimated_length=143
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1713..2358
                     /locus_tag="PLV68_00460"
     CDS             <1713..2358
                     /locus_tag="PLV68_00460"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HRD99734.1"
                     /translation="LQREGVDVVARRAGRTGTIVVLLDQRGERTMLTDRGAASQLDRP
                     DPAWLDGLSALHIPFYSLLSDPLATTARTLAGWAHERGITVSVDASSAAALREVGIDA
                     AVDLLTSLPTDVLFCNELEAAALGDEVVPERFGGRFSVVKHGRAPAVVHQPGLAPVEV
                     AAVEIGGVTDTTGAGDSFAAGVLVALAAGASPTDAVAAGHHTAARYISAASNLA"
     gene            complement(2436..2747)
                     /locus_tag="PLV68_00465"
     CDS             complement(2436..2747)
                     /locus_tag="PLV68_00465"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HRD99735.1"
                     /translation="MTIIRINAITVPPQAGPELEKRFAARANEVDKMPGFEGFELLRP
                     TDGSDKYFVVTRWADVASFDNWVNSRAFGHGHAQSAAAGAAPVSTHAELLSFEVVDFD
                     A"
     assembly_gap    2936..3072
                     /estimated_length=137
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3073..>3779)
                     /locus_tag="PLV68_00470"
     CDS             complement(<3073..>3779)
                     /locus_tag="PLV68_00470"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRD99736.1"
                     /translation="SPDSAGPDSTGPGNTVTNPANQDADGAYPVEIETQFGTTVIESR
                     PERIVSIGFTDGDFILALGVTPVAIREWYGDQPGGLWPWAAETAAAKAGSIEKLSSDA
                     ISVEKIAALKPDLIVGIVSGMTKAEYDDLSQIAPTIAQTDQYVEWGTPWNEIQLTIGK
                     ALGLHDEAEAIVASTRALIDEAAQAHPEWTGKTAQFGVVGPDGTWYSYGEQDNRGRLL
                     TELGFVVVRMDGMGTNW"
     assembly_gap    3780..3809
                     /estimated_length=30
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3810..4022)
                     /locus_tag="PLV68_00475"
     CDS             complement(<3810..4022)
                     /locus_tag="PLV68_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99737.1"
                     /translation="MTLHPAALLGFPIMTATRNPRLRRRRVTAIAMICIAGLVAAACG
                     SDSTASSTTSSTSASSTTQSDGGSASS"
     assembly_gap    4233..4386
                     /estimated_length=154
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4387..4714
                     /locus_tag="PLV68_00480"
     CDS             <4387..4714
                     /locus_tag="PLV68_00480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99738.1"
                     /translation="ERGEHPSFGVVTALGMVVARVGPNVVHLFATPLAVNEVHLLVPK
                     MAAATINIAWDPVSCMSLVYSETTLGVAAAGVADTTAAMEDLMLRCAIEEISTAVAAE
                     ASRAGG"
     assembly_gap    4769..4778
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4779..5865
                     /locus_tag="PLV68_00485"
     CDS             <4779..5865
                     /locus_tag="PLV68_00485"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HRD99739.1"
                     /translation="DVVARPRSPGAEKVPGAEVPARGLRARRRRLTLLSAGLFVAAGL
                     LVLVLFFSVSYGSKEIPFRDVIRSFTDYDPTSNNHLIIRTLRIPRTAVGLMVGACLGL
                     SGAVMQGLSRNPLADPGLLGVDAGAALFVVLGISVFGVSSLYSYVWFAFAGAFAASVV
                     VYALGSAGRTGATPVKIALAGAALTALLSSFTSGILVLDASTLDQFRFWAVGSLAGRT
                     SSLAWQMLPFAIVATVASLFTARTLNSLAMGEDTAKSLGLNINRARGLCALAIVLTAG
                     TATAAAGPIGFIGLTIPHVVRKSTGPDHRWLLPYCALLGPILLLGSDVIGRVVDRPAE
                     VQVGIITALIGAPFFIYLVRRTRLAQL"
     gene            6021..>6837
                     /locus_tag="PLV68_00490"
     CDS             6021..>6837
                     /locus_tag="PLV68_00490"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HRD99740.1"
                     /translation="MIRAGRFSARLDGRAVSVTTSLLLAALVVFCVNISVGDFNIPFV
                     DVLKYLIGDGNKDSEFIIGRLRLPRSLTGLLVGAALGLSGAIFQSIARNPLASPDVIG
                     VTAGASAFAVAMIIIGPTNDLISDVARNSVSIAALFGGLFTALMVYLLAYRRGLVGYR
                     LVLVGIGISAMMTSVISYLMTRATLYEASRATVWLTGSLNGRGWHHVRPVTAALVVLV
                     PCTMMLSRHLRALQLGDDTARALGSRTEWARLGLIVCGVGLAALATASAGPVAF"
     assembly_gap    6838..7042
                     /estimated_length=205
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7062..>7328
                     /locus_tag="PLV68_00495"
     CDS             7062..>7328
                     /locus_tag="PLV68_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRD99741.1"
                     /translation="MESVTHRTTPAPTAPTGGSRLMAEGLSLAYDDRLIVDSLSVRIP
                     TGKITIIVGANACGKSTLLRGLARLLKPRAGTVLLDGEAIHSQST"
     assembly_gap    7329..7528
                     /estimated_length=200
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7529..7901
                     /locus_tag="PLV68_00500"
     CDS             <7529..7901
                     /locus_tag="PLV68_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRD99742.1"
                     /translation="LSGGQRQRAWIAMALAQGTPIVLLDEPTTFLDLAHQADVMDLLE
                     ELNRRDGRTVVVVLHDLNQACRYADRIIAMKAGTIVAEGSPNEVITEEIVHEVFGLHA
                     RIIPDPLTETPLVIPIPKAMR"
     gene            8075..8410
                     /gene="rpsF"
                     /locus_tag="PLV68_00505"
     CDS             8075..8410
                     /gene="rpsF"
                     /locus_tag="PLV68_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HRD99743.1"
                     /translation="MMRAYELMVILDGDLEDTAAQAWVKSITDDIGKAGGSVHGRADW
                     WGRRQFAYPINKKEYGYYAVYNLLAPGGALDELERQLRLADDVVRHKLLRLPDAEAAR
                     RGVVAGSVA"
     assembly_gap    8428..8483
                     /estimated_length=56
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8484..8966
                     /gene="ssb"
                     /locus_tag="PLV68_00510"
     CDS             <8484..8966
                     /gene="ssb"
                     /locus_tag="PLV68_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012784138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HRD99744.1"
                     /translation="RDPELRFTQSGRGNATFGVAVSRRYQQNNEWVEQTSFFNVVAWG
                     TLGENAASSLQKGARIIVTGRLEQRSFDTQQGERRSVVEIVADEIGPSLRWATATVER
                     TSRDASARAATRVAVVATRATRAAATPVVMVVAAAVSRPTATKSRSEIFQFSRPLDTR
                     "
     gene            8963..9211
                     /gene="rpsR"
                     /locus_tag="PLV68_00515"
                     /pseudo
     CDS             8963..9211
                     /gene="rpsR"
                     /locus_tag="PLV68_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006303578.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
     gene            9361..9807
                     /gene="rplI"
                     /locus_tag="PLV68_00520"
     CDS             9361..9807
                     /gene="rplI"
                     /locus_tag="PLV68_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HRD99745.1"
                     /translation="MKVILRSDHKSLGKRGDIVDVADGHARNHLLPHGLAIPASDGAI
                     DQANRMRRARDVRDASARDAAQNIAKSLVAQVITIPVKVGAGERLYGSVTNADVAAAV
                     LEQAGIELDRHDLHINEAIKTLGEHSVSAKLHSEVEFPITINVVKR"
     gene            10109..11446
                     /gene="dnaB"
                     /locus_tag="PLV68_00525"
     CDS             10109..11446
                     /gene="dnaB"
                     /locus_tag="PLV68_00525"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HRD99746.1"
                     /translation="MAARRGDGRVPPHNLDAEESLLGALLLSRDALNTVAEAGVNVAD
                     FYKPAHQYIYDAIRGLSATGNPVDAVTVADELRRAGLLDEIGGLELLVDLQNATPAIS
                     NVNSYARIVRDTAVLRKLIGVASDIAELAYGEPDDVAKAIDEAESKVFEIAEDRVTDS
                     VKPLGDLMPLTLDALQATYDRGSSLTGLATGYIELDKMLSGLQPSTLNIVGARPAMGK
                     TAFALGLAVHAAQTARVPVLVFSLEMGHAELTQRILSSEARIDSSKLRDGRLVEADWT
                     KLGRAVGRLEIPLYLDDNPRVTVMEIRAKARRLKAREGLGLIVIDYLQLMSGTNTESR
                     QLEVSEISRGLKVLARELQIPIVALSQLSRNLENRSEKKPMLADLRESGSLEQDADVV
                     MFLYRDEVYNKESPDKGSAEVIIAKHRSGPIGDCKLAFLGQYTRFENMATDRN"
     gene            11471..11728
                     /locus_tag="PLV68_00530"
     CDS             11471..11728
                     /locus_tag="PLV68_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99747.1"
                     /translation="MLGLIGSTGAGSGDGSLNLLGDDLLQYLVLALGGALLVGNVLAL
                     VKPPPAPKDGDLDRAPRGRSIGMALIGLVAVVWALATLLTG"
     assembly_gap    11793..11848
                     /estimated_length=56
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11849..12332
                     /gene="yihA"
                     /locus_tag="PLV68_00535"
     CDS             <11849..12332
                     /gene="yihA"
                     /locus_tag="PLV68_00535"
                     /inference="COORDINATES: protein motif:HMM:TIGR03598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HRD99748.1"
                     /translation="GKSSLINAIANRKQLARVSKTPGRTQLINLFEMAPGATVVDLPG
                     YGFAKVPGRVKSTWGPMIERYLLGREGLRMVMVLVDGEIGPTPLDVQMLDWLRQHDVP
                     HQVVATKSDKVKSAKKPSRRKALAAGCQLEPGDIVWVSAAKGINIDRLHELLRLWLSP
                     "
     gene            12465..>12816
                     /locus_tag="PLV68_00540"
     CDS             12465..>12816
                     /locus_tag="PLV68_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99749.1"
                     /translation="MSALRVVRVRRAALALLTIGLVTPAIQLVGAPPPAAAEDIVTRV
                     VTPSNLDGWTWLTDGSSQDNMPVLPLFTADHDAGAGVGAASFPTVPITLPSPVIQATQ
                     PRMIVTNEFAGTRLA"
     assembly_gap    12817..12928
                     /estimated_length=112
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <12929..>13160
                     /locus_tag="PLV68_00545"
     CDS             <12929..>13160
                     /locus_tag="PLV68_00545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99750.1"
                     /translation="TSGWKTWDTLELGAGHWTTTNPAGGTCVHANCTWAQVLAQFPNA
                     TINNPTNALVGRLLIRGGDTSTDTSAMTLHTDM"
BASE COUNT         1857 a         4027 c         4077 g         2020 t
ORIGIN      
        1 gagcgacgtc aaggcggcgt cggtggcgct gcgcgatctg cgcagtctcg gcctgcacct
       61 gtcggtcgac gacttcggca ccggctactc gtcgttgacc tacctcaagc ggttcccggt
      121 cgaagcgatc aaggtcgacc gcaccttcgt caaggggttg ggcatcgacg cggaggacac
      181 gacgatcgtc gaggcggtag tcaacctcgg ccggtcgctc ggtctgcgcg tggtggccga
      241 aggcgtggag acgccgctgc agctgtcacg gttgcgggag atgggttgtg cctacgccca
      301 gggctacctg ttcggccggc cccgccccgc ggcgttgatc gaggccgagc gggcaggctc
      361 gttcgcgccg tagttccggt gggtcgggtc agacgcggcc gggtctgttc agccgcggcg
      421 ctggcggcca ccgaggggaa tgacccgcag caggtggtgc cagtggcagg ccgtgcaggc
      481 ctcgaccacg taggcgtcga ggtcgtcggc acgagcgtcg agttggtcca tgtcggcctt
      541 ggtcgacacg caacgaccnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngttgct ctgcgtgccg acgttcttgg
      661 cggcccggat cagttcgggg tgggcatcgc agatctgctc gcgccgcagg cggccgcggc
      721 ggtattcgtt gaccaacgcc agccgctcga ggcggtggtc gaccacggcg cggcggctgt
      781 gctgaccggc gctggggcga ccgctgcgga aggcatcgac cgaaaacgac attgccggac
      841 acgctatccc gtgctggtcg agcgagatcc acgttgagcg gtcgtgcacc gaccacactg
      901 gcggggtggc agtcgtacgt tatggaacct caatcgccga cgatgccgag ctgcgcctga
      961 tcggcgacgt caccggcaag cgggtgttgg agctgggcat cagcacgccg tccaatgcgg
     1021 tggtcctggc cgccaacggg gcacgggcac tgcccgccga tccgtcggcc gatcgcatcg
     1081 ccgcgctgcg tcgtgaggcc gaagcagccg aggtcacggt gcagtgtcac caggtcgcgg
     1141 tcgccgagct gggaccgatc gcgagcgcgt ccatcgacgt ggtcgtcgcc gcccacagcc
     1201 tcgacgaggt cgacgacctc gcccgcgtgc tgcgccaggt gcaccgcatc ctcaagcccg
     1261 acgccagctt cgtcgtggcg gtgcgtcatc ccatcgacgc aatcgtgacc agtgctgatg
     1321 gcaacacgat cgtcaccatt ccctacggag ccggtggcgt gcgttcgatc ggcgactggt
     1381 tcatgtcgat gcagcgcacc aacttcgcga tcgacgtcgt ccacgagttg cgttcgatcg
     1441 gcgcggcgca ctcgctgaca ccgacgacct tggtgctgcg cgccaagaag ctcggcgtgt
     1501 gacccagcac gtgatcggca caatcggcga tctggtcgag gacatcctcg tccacctcca
     1561 cggcccggtn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngttacagc gggagggggt cgacgtcgtc
     1741 gcccggcgag ctgggcgcac cggcacgatc gtcgtgctgc tcgaccagcg cggcgagcgg
     1801 acgatgttga ccgatcgcgg cgcggcgtct cagctcgacc gtcccgaccc ggcttggctc
     1861 gacggcttgt cggcgctgca tatcccgttc tattcgctgc tcagcgaccc gctggccacc
     1921 acggcgcgca cgctggcggg ttgggcccac gagcgcggta tcacggtgtc ggtcgacgcg
     1981 tcgtcggccg ccgcgctgcg tgaggtcggc atcgacgccg cggtcgacct gttgaccagc
     2041 ctgcccaccg acgtgttgtt ctgcaacgag ttggaggcgg cggcgttggg ggacgaggtg
     2101 gtgcccgagc gcttcggcgg ccggttcagc gtggtcaagc acggccgcgc ccccgccgtg
     2161 gtgcatcagc ccggcctggc tccggtcgaa gttgcggcgg tcgagatcgg tggtgtcacc
     2221 gacacgaccg gcgcgggcga ttcgttcgca gccggtgtgc tggtcgcgct ggccgccggt
     2281 gcgtcgccga cggacgcggt ggccgcgggc caccacaccg cagcccgcta catcagcgcc
     2341 gcttccaacc tggcttgagg acgcccgacc tgctcccggt ccggcctgac ctggcctgat
     2401 ctgacctgac ctgacttgtt ggggtgatgg ctggttcagg cgtcgaagtc gaccacttcg
     2461 aagctgagca gttcggcgtg ggtcgacacg ggggccgcac cggccgcggc ggactgggcg
     2521 tgaccgtggc cgaacgcgcg gctgttgacc cagttgtcga acgacgcgac gtcggcccaa
     2581 cgagtgacga cgaagtactt gtccgatccg tcggtcgggc ggagcagctc gaacccttcg
     2641 aaccccggca tcttgtcgac ctcgttggcg cgggcggcga agcgcttctc cagttcgggt
     2701 ccggcctgcg ggggcacggt gatggcgttg atgcggatga tcgtcatcgg gtcagtccaa
     2761 tcgttcgggc cagtaccggt caactccgcg accggtcaac tccagtgcgg gtcgactcca
     2821 gtacgggtca atccagggcg gcttcgatct gggggacgag caggccgacg gcgtacggca
     2881 ggctcagcgt cgtcgcgaac gacatcgccc ccgctgttgc cgggtcggcg tagacnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnccagttcg ttcccatgcc gtccatccgc acgacgacga agccgagttc
     3121 ggtcaggagc cggcctcggt tgtcctgctc gccgtacgaa taccacgtgc cgtcggggcc
     3181 gacgacgccg aactgagcgg tcttgcccgt ccactcaggg tgggcctgcg cggcctcgtc
     3241 gatgagcgca cgggtggagg cgacgatcgc ttccgcctcg tcgtgcaggc cgagcgcctt
     3301 gccgatggtg agctggatct cgttccacgg cgtgccccat tcgacgtact ggtcggtctg
     3361 ggcgatggtc ggggcgatct gcgacaggtc gtcgtactcg gccttggtca tgccggagac
     3421 gatgccgacg atcaggtcgg gcttgagtgc ggcgatcttc tcgacagaga tggcgtcgga
     3481 ggacagcttc tcgatcgaac ccgccttggc agcagcggtc tcggcggccc acggccacag
     3541 gccacccggc tggtcgccgt accattcgcg gatcgcgact ggtgtgacgc cgagggcgag
     3601 gatgaagtcg ccgtcggtga agccgatcga cacgatccgc tccgggcgtg actcgatcac
     3661 ggtcgtgccg aactgggtct cgatctcgac gggatacgcg ccgtcggcgt cctggttggc
     3721 cgggttggtg acggtgttgc cggggccggt gctgtcgggg ccggcgctgt cggggctggn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnna ctcgatgccg aaccgccatc cgactgtgtc
     3841 gtcgatgacg cgctggtcga cgaggtcgtg ctgctggcgg tcgagtcgct gccgcacgcg
     3901 gcggcaacga gaccagcgat gcagatcatg gcgatcgcgg tgacgcgacg tcgccgcagg
     3961 cgagggttcc gggtggcggt cataataggg aaccctaaca gtgcagccgg gtggagggtc
     4021 aagggcccta cgtggtttgt acctcgtggt gtttaccgac catgtaaaga tcgcctaaca
     4081 tatggtccat gtgtcccgcc gccttccccc gctctcgatc gtgaccccgc tcggcttggt
     4141 gacgatgcgc aaggcggccg cgctcgcctt cgccgtcccc ggggtggcga tcgacatcac
     4201 ccatcgggat gcagtgatgg tgcacgtcgg acnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4381 nnnnnnggag cgaggcgagc atccatcctt cggtgtcgtc accgcgctgg gcatggtggt
     4441 ggctcgtgtc gggccgaacg tggtccactt gttcgccaca cctctcgcgg tcaacgaggt
     4501 ccacctactc gtccctaaga tggccgccgc gacgatcaac atcgcatggg accccgtgtc
     4561 gtgcatgtcg ctcgtctact ccgaaaccac gctcggcgtc gcggccgcgg gcgttgccga
     4621 caccaccgcc gcgatggagg acctgatgtt gcgctgtgcg atcgaggaga tcagcaccgc
     4681 cgttgctgcg gaggcgtcga gggccggcgg ttgacgtgac atccggcccc acggccatca
     4741 cggtgcagtc agccgatgtg ctgccgggnn nnnnnnnntg acgtcgtcgc tcgaccaagg
     4801 agtcctggcg cagagaaggt tcccggcgca gaggtacccg cacgagggct gcgcgcgcgt
     4861 cgtcgccggc tgacgctgct gtcggccggg ttgttcgtcg cggccggcct gctcgtgctg
     4921 gtgctcttct tctccgtctc ctacgggtcg aaggagatcc ccttccgcga cgtgatccgc
     4981 tcgttcaccg actacgaccc cacgtcgaac aaccacctca tcatccgcac gttgcgcatt
     5041 ccgcgcaccg cggtggggct gatggtcggc gcctgcctcg gcctgtccgg cgccgtgatg
     5101 caagggctgt cgcgcaaccc gctggccgat ccgggcctgc tcggcgtcga cgccggggcg
     5161 gcgctgttcg tcgtgttggg catctcggtg ttcggcgtat ccagcctgta cagctacgtc
     5221 tggttcgcct tcgccggtgc cttcgcggca tcggtcgtgg tgtacgcgct gggctcggcc
     5281 ggacgcacgg gcgcgacgcc ggtcaagatc gcgctcgctg gcgcggcgct gaccgcactg
     5341 ctcagttcgt tcacgtcggg catcttggtg ctcgacgcgt cgacgctcga ccagttccgc
     5401 ttctgggcgg tcggttcgct ggccgggcgc accagttcgc tcgcctggca gatgttgccc
     5461 ttcgccatcg tcgccaccgt ggcctcgctg ttcacggcgc gcaccttgaa ttcgttggcg
     5521 atgggtgagg acacggccaa gtcgctcggg ctcaacatca accgggcgcg tggcctgtgc
     5581 gccctggcga tcgtgctcac cgctggcacc gccaccgcgg cagccggacc gatcggattc
     5641 atcggcctga cgatccctca cgtggtgcgc aagtccaccg ggcccgatca ccggtggctg
     5701 ctgccgtact gcgccctgct cgggccgatc ctgctgctcg gcagcgacgt gatcgggcgt
     5761 gtcgtcgacc gtccggccga ggtgcaggtc ggcatcatca ccgcgctgat cggcgcaccc
     5821 ttcttcatct atctcgtccg ccgcacccgg ctggcccagc tatgaccaca tcgaccccgg
     5881 ctacatcgac cccggctaca tcgacaccgg ctacatcgac accggtcgag gtgccccgcg
     5941 actcgtcgcg aggtgacgct ccgccgtcat cgtcgggatc tgcatcggct cggttcatcc
     6001 gccgccgagg ccgcttcgtg gtgatccgcg ccggacggtt ctcggcccgc ctcgacgggc
     6061 gggccgtctc ggtgaccacc tcgctgctgc tcgccgcgct ggtggtgttc tgcgtcaaca
     6121 tcagcgtcgg cgacttcaac atccccttcg tcgacgtgct caagtacctg atcggcgacg
     6181 gcaacaagga ctcggagttc atcatcggcc gcctccgcct accgcggtcg ctgaccgggc
     6241 tgctggtcgg tgccgcactg ggcctgtcgg gggcgatctt ccagtcgatc gctcgcaacc
     6301 cgctggcgtc gcccgacgtc atcggcgtca ccgcgggtgc ctcggcgttc gccgtcgcga
     6361 tgatcatcat cgggccgacc aacgacctga tcagcgatgt cgcccgcaac agcgtgtcga
     6421 tcgcggcgtt gttcggcggt ctcttcactg cgctgatggt gtatctgctc gcctaccgcc
     6481 gcggcctggt cggctaccgg ttggtgctgg tcggcatcgg gatcagcgcc atgatgacct
     6541 cggtgatcag ctacctgatg acccgtgcca ccttgtacga ggcgtcgcgg gcgacggtgt
     6601 ggctgaccgg ttcgctcaac ggccggggct ggcatcacgt ccgcccggtc acggcggccc
     6661 tggtcgtgtt ggtgccgtgc acgatgatgc tgtcgcgcca cctccgcgcg ctgcagttgg
     6721 gcgacgacac cgccagggcc ctggggagtc gcaccgagtg ggccaggctc gggttgatcg
     6781 tgtgcggcgt cggcttggcc gcgctggcca ccgcgtcggc ggggcccgtg gccttcgnnn
     6841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7021 nnnnnnnnnn nnnnnnnnnn nntcggctcg ggaggctgac cgtggaatcc gtcacccacc
     7081 gcacgacccc cgcgccgact gcgccgaccg gcgggtcgcg gctcatggcc gaaggcctca
     7141 gcctggccta cgacgaccgc ctgatcgtcg actcgctgtc ggtgcgcatc cccaccggca
     7201 agatcacgat catcgtcggg gccaacgcgt gcggcaagtc gacgctgttg cgcggcttgg
     7261 ctcgtctgtt gaagccacgg gcgggcacgg tgctgctcga cggtgaggca attcactcgc
     7321 aatcgacgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7501 nnnnnnnnnn nnnnnnnnnn nnnnnnnngt tgtccggtgg gcagcgtcag cgggcctgga
     7561 tcgcgatggc gttggctcag gggacaccga tcgtgctgct cgacgagccg acgacatttc
     7621 tcgacctagc ccatcaggcc gatgtgatgg acctcctcga agagctgaac cgacgtgacg
     7681 gacgcaccgt ggtggtcgtg ctgcacgacc tcaaccaggc ctgtcggtat gcggaccgca
     7741 tcatcgccat gaaggcgggg acgatcgtgg ccgagggcag ccccaacgag gtgatcaccg
     7801 aagagatcgt ccacgaggtg ttcggcctgc acgcccggat catccccgac ccgctcaccg
     7861 agaccccgct ggtgatcccc attcccaagg cgatgcgctg aacaacggtg acgtggtgat
     7921 ctgtatgaca cccctgtggt tgagtgccgg gcatgagcac accggccgtt tcaggggctg
     7981 catccgctag catcgccacg tccatattca ccctgccccg tccaggggcc ccgctcggaa
     8041 aggccgtgcg ggtccgaagg gaggttcccc acgcatgatg cgtgcgtatg agttgatggt
     8101 catcctcgac ggtgatctcg aagacacggc tgcccaggct tgggtcaagt ccattaccga
     8161 cgacatcggc aaggccggtg gctcggtgca cggccgggca gattggtggg gtcggcgcca
     8221 gttcgcctat ccgatcaaca agaaggagta cggctactac gccgtctaca acctgctcgc
     8281 cccgggcggt gccctcgacg agctcgagcg tcagctccgc ctcgcggacg atgtcgtccg
     8341 ccacaagctc ctccgcctgc ccgatgccga agcggcccgc cgcggcgtgg tggccggcag
     8401 cgtggcctga tccaggcccg ccacaccnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8461 nnnnnnnnnn nnnnnnnnnn nnnagagatc ccgaactgcg cttcacgcag tccggccgcg
     8521 gcaacgccac cttcggcgtg gccgtcagcc gccggtacca acagaacaac gaatgggtcg
     8581 agcagacctc gttcttcaac gtcgttgcct ggggcacgct cggcgagaac gccgcgtcgt
     8641 ccttgcagaa gggcgcccgc atcatcgtca ccggccgtct cgagcagcgt tcgttcgaca
     8701 cccagcaggg tgaacggcgt agcgtggtcg agatcgtcgc cgacgagatc ggccccagct
     8761 tgcgctgggc cacggccacg gtcgagcgca ccagccgcga tgcgtcggcc agggcggcaa
     8821 ccagggtggc ggtggtggct accagggcaa ccagggcggc ggctactcca gtggtaatgg
     8881 tggtggccgc ggcggtgagt cggcctacgg cgacgaagag ccgttctgag attttccagt
     8941 tttcgagacc tttggatacg agatgaccag caagaagaac aaggcccgca gtccgcgcga
     9001 ggccaacccc aagaagttca agaagaagac cagcgtgctc gtcaccgaca aggtggagta
     9061 catcgactac aaggacgtca acctgctcag ccggttcgtc agtgaccgct ccaagatccg
     9121 caaccggcgg gtcacgggca acaacgtgca gcagcaacgt gagatcgcca acgcgatcaa
     9181 gaactcgcgc gagatggcgt tgctgccgta caccaagcgc gtcgccagca cccgcccggg
     9241 tcgcccaccg cgcgacggtg agcgtgggcc gcgccgcgat gccgaggtgg ccgaagagac
     9301 caccgaagac accggcgtcg acgatgccga taccgacggc gaagacacgg aggactgatc
     9361 atgaaggtca tcttgcggag cgatcacaaa tccctgggca agcgcggcga catcgtcgat
     9421 gtcgccgacg gccacgcccg taatcacctg ttgccgcacg gtctggccat ccccgccagc
     9481 gatggtgcga tcgatcaggc caaccgcatg cgccgggccc gcgacgtccg cgacgccagc
     9541 gcccgcgacg cggcccagaa catcgccaag tcgttggtgg cccaggtcat caccatcccg
     9601 gtcaaggtcg gagcgggcga gcggttgtac ggctcggtca ccaacgccga cgtcgccgcg
     9661 gccgtgctcg aacaggccgg catcgagctc gaccgccacg acctacacat caacgaggcg
     9721 atcaagaccc tcggcgagca ctcggtcagc gccaagctgc acagcgaggt cgagttcccg
     9781 atcacgatca acgtcgtcaa gcgctaacac caatcggcgc cgaaggcgcc gggggatcgc
     9841 gttggggtgt tctgatcaac gtgtcgcctc tcgcgccgtc gatgtgtgac ttgctgcctc
     9901 actctccgtc cacactgggc gagagggtga ggcagtcttc attacaacgg tgttgggcgg
     9961 ttgtccacat ggaggaggga gtcatcgaca acttcgtcac acccttgtgc cacttgtgat
    10021 ccacagaccg accgcgaaag ggttgcaggg ttatgtctca ggctgacaac gcacgtccac
    10081 gccccgctcg ccgcggctgg gggcgcgcgt ggccgctcgc cgcggcgacg gacgcgtccc
    10141 cccgcacaac ctcgatgccg aagagagcct gctcggtgcg ttgctgttgt cgcgcgacgc
    10201 gctcaacacg gtggccgaag ccggcgtcaa cgtggccgac ttctacaaac cggctcacca
    10261 gtacatctac gacgcgatcc gcggcctgtc cgccaccggc aacccggtcg acgcggtcac
    10321 cgtggccgac gagttgcgcc gggccgggct gctcgacgag atcggtggcc tggaactgct
    10381 ggtcgacctg cagaacgcca cgccggcgat ctccaacgtc aacagctacg cccgcatcgt
    10441 gcgcgacacg gcggtgttgc gcaagctgat cggtgtcgcc tccgacatcg ccgagcttgc
    10501 ctacggcgag cccgacgacg tggccaaggc gatcgacgaa gccgagtcga aggtgttcga
    10561 gatcgccgaa gaccgcgtca ccgattcggt caagcccctc ggcgacctga tgccactcac
    10621 cctcgatgcg ctgcaggcca cctacgaccg gggcagttcg ttgaccggtc tggccaccgg
    10681 ttacatcgag ctcgacaaga tgctctccgg cctgcagccg tcgacgctca acatcgtcgg
    10741 cgcccggccg gccatgggca agaccgcgtt cgccctgggc ctggcggtgc acgcggcgca
    10801 gacggcgcgg gtgccggtgc tggtgttctc gttggagatg ggccatgccg agctgaccca
    10861 gcgcatcctc agcagcgagg cccgcatcga ctcgtccaag ctgcgtgacg gtcgcttggt
    10921 cgaagccgac tggaccaagc tcggccgggc ggtcggccgg ttggagatcc ccctctacct
    10981 cgacgacaac ccgcgggtca cggtgatgga gatccgggcc aaggcgcgcc ggctcaaggc
    11041 tcgcgagggc ctgggcctga tcgtcatcga ctacctccaa ctgatgagcg gcaccaatac
    11101 cgagagccgc cagctcgagg tcagcgagat cagccgtggc ctcaaggtgt tggcccgcga
    11161 gttgcagatt ccgatcgtcg cgctcagcca gctgtcccga aacttggaga accggtccga
    11221 gaagaagccg atgctggccg acctgcgtga gtccggctcg ctcgaacagg acgccgacgt
    11281 ggtcatgttc ttgtaccgcg acgaggtcta caacaaggaa tcgcccgaca agggctcggc
    11341 cgaggtgatc atcgccaagc accgttccgg acccatcggc gactgcaagc tcgccttcct
    11401 cggccaatac acccggttcg aaaacatggc caccgaccgc aactgacccc taccccgcca
    11461 gactgggggt gtgctggggt tgatcgggtc gactggtgct gggtcggggg atgggtcgct
    11521 caatctgttg ggcgacgatc tgctgcagta cttggtgctc gcgttgggtg gggcgctgct
    11581 ggtgggcaac gtgttggcgc tggtcaagcc gccgcccgca cccaaagacg gcgatctcga
    11641 tcgggcgccg cgggggcgca gcatcggcat ggcgctgatc gggctggtcg ctgtggtgtg
    11701 ggcgctggcg acgctgctga ccgggtagac cgggcgggga caggggagca aggagcgagc
    11761 atggcgacca ttccgttgca gttcgtgcag tcnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11821 nnnnnnnnnn nnnnnnnnnn nnnnnnnngg gcaagtcgtc gctgatcaac gcgatcgcca
    11881 accgcaagca gttggcccgg gtcagcaaga cccctgggcg gacccagttg atcaacctgt
    11941 tcgagatggc ccccggggcc acggtggtcg acctgcccgg ctacgggttt gccaaggtgc
    12001 cgggccgggt gaagagtacc tggggaccga tgatcgagcg ctacctgctc ggccgcgagg
    12061 gcttgcgaat ggtgatggtg ctggtcgacg gcgagatcgg ccccaccccg ctcgacgtgc
    12121 agatgctcga ctggctgcgc cagcacgacg tcccccacca ggtggtcgcg acgaagagcg
    12181 acaaggtcaa gtcggccaag aagccgtcgc ggcgcaaggc gctcgcggct ggctgccagt
    12241 tggaaccggg cgacatcgtg tgggtcagcg cggccaaagg catcaacatc gaccgcctcc
    12301 acgagttgct ccgcctctgg ctatcgccgt agctcacgcg agacgaactt cacctacagt
    12361 ttgcgcacgc gccatgtcga taggttactc tcgcggccct ggacgggatt gcacgcccgt
    12421 cgattcgtgt aactagcggg tcgggggtgc gggaagggga cagaatgagc gctttgcgtg
    12481 tggtacgggt ccgccgcgcg gccttggcac tgctgaccat cggcttggtg acgccagcca
    12541 tccaactggt cggggcccca ccgcccgcgg cggctgagga catcgtcacc cgtgtggtga
    12601 cgccgtcgaa cctcgacggc tggacgtggc tgaccgacgg gtcgagccag gacaacatgc
    12661 ccgtgttgcc gttgttcacc gccgaccacg acgccggtgc gggcgtcggc gcggcgtcgt
    12721 tcccgaccgt gccgatcacg ctgccctcgc cggtcatcca ggcgacccag ccgcggatga
    12781 tcgtcaccaa cgagttcgcc ggcacccgcc tggccgnnnn nnnnnnnnnn nnnnnnnnnn
    12841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnca ccagcggctg gaagacgtgg gacaccctcg
    12961 aactgggcgc cgggcactgg accaccacca accccgcggg cggcacctgt gtccacgcca
    13021 attgcacctg ggcgcaggtg ctggcccagt tccccaacgc cacgatcaac aaccccacca
    13081 acgcgttggt cgggcggctg ctgatccgcg gtggcgacac ctccaccgac accagcgcga
    13141 tgacgctgca caccgacatg
//
LOCUS       DAORNF010000006        12552 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_3794_length_12552_cov_1.930863, whole genome shotgun sequence.
ACCESSION   DAORNF010000006 DAORNF010000000
VERSION     DAORNF010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 12552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12552
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3794_length_12552_cov_1.930863"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /locus_tag="PLV68_00550"
     CDS             complement(<1..357)
                     /locus_tag="PLV68_00550"
                     /inference="COORDINATES: protein motif:HMM:NF024966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HRD99751.1"
                     /translation="MADTHWTITNLLYRYAECIDAGDFEGVGDILAHATLTAEGTALH
                     LDTRAAVLAHYVNVTRRYPDGTPRTKHVTTNPIVEVDEAAGTATCRSYFTVLQAVDGS
                     LALQPIIAGRYHDSFER"
     gene            411..1085
                     /locus_tag="PLV68_00555"
     CDS             411..1085
                     /locus_tag="PLV68_00555"
                     /inference="COORDINATES: protein motif:HMM:NF018777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EthD domain-containing protein"
                     /protein_id="HRD99752.1"
                     /translation="MTTALLLLAGDPEVIRTALGELHPDDAVHRWAGIRAPDDPMANT
                     VYGSRWPYGGFVALEYPDTEPPGLGERLSTLVGSLGEAIDPSRSAVLVGTTHRIIDGR
                     TDVALVYLIHRIGGLTVEQYQDHWLNQHGPLAKALVPTSGYEQTHADHDGGAALASRL
                     GFATPDFDGNATCFFDTAQDFADMLTARESSRDMNVIYEDELRFLDHDRSLGALMRQV
                     PLGAAP"
     gene            1115..>1383
                     /locus_tag="PLV68_00560"
     CDS             1115..>1383
                     /locus_tag="PLV68_00560"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRD99753.1"
                     /translation="MATLTLDSPGNRNALGMQLTTELRDHLTTCLRDAAVRVIVITAT
                     GTVFSSGWDLDQEPSQGLLVDDVLVEVLEAIRTGPKTVVAKIGGHA"
     assembly_gap    1384..1393
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1394..1846
                     /locus_tag="PLV68_00565"
     CDS             <1394..1846
                     /locus_tag="PLV68_00565"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRD99754.1"
                     /translation="VGGALGLVAACDLSIAAADVRFGFGEVRLGLAPTTAAVHCLPKM
                     RRADALELLLTGERFGAQRAAEAGIINRVVAPESLDDSVAGLVAALILGGPIALGACK
                     RLARTITDAGPDAYALARELNAALVGSPEATEGIAAFLDRRPPVWART"
     gene            complement(1857..3179)
                     /locus_tag="PLV68_00570"
     CDS             complement(1857..3179)
                     /locus_tag="PLV68_00570"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HRD99755.1"
                     /translation="MLAGYFTERPYRGLSEEEILQHGSYFSMPNSKFDPAIASRDYNY
                     YLDENCYAEELGFDAVALNEHHGNPFCMGSTMNLEAAILARITERVRLLLIGNPLPAH
                     RNPLRMAEELAEIDLISRGRLIAGWVRGSGPEQFFNNTNPAHNREMFEEALAFVEQAW
                     TRPGPWRYEGNHFHYRHVNPWVLPYQKPRPPTVIPGVLSLETIEWAAEHAYPYLGLGT
                     SLGPTAELWNSYADRAAECGYQAGSENFAYLTMASVAATEEEAIEVARYHLYANGNGS
                     FARPEYTLPAGFNSPAAIKRLAKMPSSGWLGVSREKLNGSDTGRGEFDMAKAKSRIDE
                     SFHRHVRNMQIVAGTPDQVIPKIKQMVSVLRPGLMVFMNVQGNCSNERRRENMRLIAN
                     EVLPEIRAHSKALGLQSMLDVEPGSRRLEPGQQRQPVVDLSALETLVD"
     gene            complement(3192..3977)
                     /locus_tag="PLV68_00575"
     CDS             complement(3192..3977)
                     /locus_tag="PLV68_00575"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRD99756.1"
                     /translation="MTESIVDVGGTPITVLRGGAGRPLLMLHDELGFPGWMRWNEQLA
                     EQCEFVIPLQPGFGHTPRIPWLQDFRDLATFYGRLLRQLELGPTDVIGFSAGGFIAAE
                     MAVMAPDLFERMVLVAPLGVRPQSGEIFDFLASTMMTHVAATVSHHEVEEFGQIYGGD
                     MTAEQFELFEAARGETSRLGWEPFMFNPSLPHHLQGLCDLPTLVVWGEDDLIVPRGAI
                     DAYEEALPNVEVHILPAVGHRPEIEDVDGFVGCVSKFLLPRDH"
     gene            complement(4203..5057)
                     /locus_tag="PLV68_00580"
     CDS             complement(4203..5057)
                     /locus_tag="PLV68_00580"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HRD99757.1"
                     /translation="MTGTFEVRPLVMGKLHYAEGLRWHDGKLWLSDLFGRAVYTIDGD
                     RLDLQATLPDDEPSGLGFLPSGAPIVVAMKSRQLRSIAGGEVSLYADMSVASRSECND
                     MLVDGQGRAYVSNFGYDLRSGEEPKPGELILVRPGEPAVVAVGDLIFPDGMVLSPDGR
                     TLIVAQLFGEELTAYDVADDGSLSGRRTFAHLPGIAPDGLAIDAEGAVWVSGSTGCDY
                     LRVLDGGEITHRIAIDGQWTPSCALGGDDGRTLFMVSAETTLDEWMAGKSVSHIRTAR
                     VDVPAASY"
     gene            5271..>5643
                     /locus_tag="PLV68_00585"
     CDS             5271..>5643
                     /locus_tag="PLV68_00585"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRD99758.1"
                     /translation="MGTPGVQFGDQFFAQEEIFDRGARAARGLASIGVRRGDVIATYL
                     RNDIAFFETAIAAATLGVHIVPINWHFKTDEMRYVLDDCGARVLVAHTDLLAALHGDL
                     PAAVTIFSVPTIVPRRHGAPAP"
     assembly_gap    5644..5682
                     /estimated_length=39
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5683..6775
                     /locus_tag="PLV68_00590"
     CDS             <5683..6775
                     /locus_tag="PLV68_00590"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRD99759.1"
                     /translation="FEPWSDPPQTPEFSMNYTSGTTGKPKGVRREPMTDEGRAAMAAM
                     FAQLEVKSGMRTIVAAPLYHAAPFGFSMAVLRAGGLMILMDRFDAEEYLANIERYKVD
                     NIQMVPTMFVRLLKLPAEVRERYDVSSLQLVTHGAAPCPAEVKTAMIQWLGPIIKEFY
                     AASELGMIATYCSSEEALSHPGTVGRPVDGAEIRILDDAGNELPSGEVGEIAMWIRGT
                     SAFTYHGNEEKRQGIQRGNLVTCGDVGYLDADGYLYLTDRKSDMAIVGGANVYPAEVE
                     QLLVSMPGVLDCAVFGIPDDDLGEVLAAVVQRQPGATITSDDVIGWLTPRIARMKVPA
                     TVEFRDELPREDSGKLFKRRLKEEFAARR"
     gene            complement(6797..7573)
                     /locus_tag="PLV68_00595"
     CDS             complement(6797..7573)
                     /locus_tag="PLV68_00595"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRD99760.1"
                     /translation="MSAGMLDAKTVAVVGAGTGLGQEIARVVRREGGNVVLGARTASV
                     LEDIAFGVDPSGDRVMWRTTDVRDSEQCRALVTSAVERFGGLDAVVICAVAGDALVGG
                     IADADLDEWRDLFEVNLFGAMQVVSAAVPAMETQGSIVFIGSQAAYFPNVIQAGYAAS
                     KAALVGVTHHLAAELGPRGIRVNVVAPGWMWGPSLEKSLDAATGGGGAALIEKIGRKL
                     PLGTVAEDGDVAEAAAFLASARAKAITGQCLLVNAGEYTR"
     gene            complement(7570..8463)
                     /locus_tag="PLV68_00600"
     CDS             complement(7570..8463)
                     /locus_tag="PLV68_00600"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRD99761.1"
                     /translation="MGLRDVDLPILDCLSMVWPNGVGASMPIPREAFRDEESLAAWPS
                     TGIVNYLFKGAQERSRLSNEPGKVVEQYDRFNIAKGLTLVAMDDVPVVMDAIRPWADR
                     FIVGVRANPHEGMRGIRKLEQLVRDYPQIRTVSLSPHMLWPPIPPNSKEYYPIYAKCI
                     ELGLPVYMNVGIPGPRVLGDCQNPAYLDEVLWFFPDLTIVMKHGGEPWADMCVKLMLK
                     WPNLYFATSAFAPKYYAPEIIQYANTRGADRVLFAGYWPNLAYDDVFAQLEQVPLRDH
                     VWPKFLHENAARVFGVEVRSS"
     gene            8559..10241
                     /locus_tag="PLV68_00605"
     CDS             8559..10241
                     /locus_tag="PLV68_00605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HRD99762.1"
                     /translation="MGAEIIVETDVAVPMRDGTVLRADVYRPAEGPAPTLLERTPYSK
                     DQLVGTIFVMNPMRAARAGYSVVVQDVRGRFSSDGDFYPFVNESLDGYDTVEWCAAQP
                     WSTGKVGMFGSSYMAAAQWQAAKANPPHLTTITPFQASADYRDGRSYRGGAFELGSLL
                     SIALYALGAGQMARLPLAERRPMWDTLRGAIDDISATSRAVLENRLAGTVIPEIIPYF
                     FDWLDHNEPGPYWDRIDVTQHYQNIDLPVLHVSCWFDQFLVGTIRNFVGMRERAASQH
                     ARDNQHLFIGPWGHYAPRTALLGTARIGDMDLGVSAVSDLDAMLISWFDRWMKDEPIA
                     GRAIAPVRLFVTGSNTWHNLSQWPPETTATLDLFMHSEGSANSSDGDGRLDSTPPTAD
                     EAADTYLHDPADPVPTCGGAHLVLEATYPQGAVDQRDVEARDDVLVFTTHALDADVEV
                     LGPVTAHLTVESTAPSADFDVTVSDVHPDGRSLKICDGILRTILDDGPAAIVVDLGVI
                     GHRFLAGHRIRVHVASSNFPRYDLNPAAVTATQSLHHSAAHTSLLRLPISAL"
     gene            complement(10252..11094)
                     /locus_tag="PLV68_00610"
     CDS             complement(10252..11094)
                     /locus_tag="PLV68_00610"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HRD99763.1"
                     /translation="MTLTRPVTGLPAPRRFSVCQHVLRLGDLARDVEAAVGGGASAIS
                     LNAADFGGIGIAEAKQIIADSGLAVSSYIDPALMRLGPVAPDLDALERSFGDARELGA
                     PCFLFIAGDIAGRDPAAADQQLVDALSAVADRVAGSGVSPMIEPLHPIARELSYVHTL
                     SHALDLAKQVPGTGVVVDTGASWWERDLVAGVAANLELVFAVQITDVRADEMTDRRYV
                     RAQCGAASAVPVADLVHDFLAAGYEGWFEHEVLVKATPRERIEMVAADRQWFESLFGA
                     VPAV"
     assembly_gap    11309..11536
                     /estimated_length=228
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11537..12257
                     /locus_tag="PLV68_00615"
     CDS             <11537..12257
                     /locus_tag="PLV68_00615"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRD99764.1"
                     /translation="YAAAFASADPRRLRCAMIAPLNHPVQAAAEIAFAHDQLGMTVLL
                     ASFTAPGARSLSSPDLDVVWAAAHERAITVTFQDSSLSAVPTTSGIARAKTWRMLYLA
                     THIVEAQLGLADLILGGVPDRFPALRFGMQETHLTWLPSWLQLLDERFGHRRQPSVRP
                     SDAFRRQFFASGFPDEPGVRRFIDEVGGRNLVFASDWPHRELMPGTDPNWTAEVLRRN
                     DLSAAEAVAALETNPNHWFSN"
     gene            12283..>12552
                     /locus_tag="PLV68_00620"
     CDS             12283..>12552
                     /locus_tag="PLV68_00620"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRD99765.1"
                     /translation="MPRSDPPSTSRAKKASANPRTKSVAAKQPSSKTVTKRTPALKHQ
                     GRNSARRNEIVRAAAETFYSKGYDATTTQDIAEAVGMLKGSLYYYI"
BASE COUNT         2181 a         4202 c         3840 g         2052 t
ORIGIN      
        1 acgctcgaac gagtcgtggt accgcccggc gatgatcggc tgcaacgcca gcgacccgtc
       61 gacggcctgc agcaccgtga agtacgaccg acacgtcgcg gtacccgcag cctcgtcgac
      121 ttcgacgatc ggattcgtcg tcacatgctt ggtgcgcggc gtaccgtccg ggtagcgacg
      181 ggtgacgttg acgtagtggg cgagcaccgc agcacgcgtg tcgagatgca acgccgtccc
      241 ctcagccgtc agcgtggcgt gggccaagat gtcgcccaca ccctcaaagt cacctgcgtc
      301 gatgcattcg gcgtagcgat agagcagatt ggtgattgtc caatgagtgt cggccattcc
      361 ccaatggtaa gtgaatgaaa taggtcgatc gacacaacgg aggtatttct atgacaaccg
      421 cactgctcct gctcgcaggc gacccagagg tcatcaggac cgcgctcggt gaactccatc
      481 ccgacgatgc cgtgcaccgt tgggccggca tacgcgcccc ggacgatccg atggcgaaca
      541 ccgtgtatgg gtcgcgctgg ccgtacgggg gcttcgtcgc tctggagtac ccggacaccg
      601 agccgcccgg actgggcgag cggctgagca cgctcgtcgg ttcgctgggc gaagcgatcg
      661 acccctctcg ctctgcggtg ctcgtcggga cgacacaccg catcatcgac gggcgcaccg
      721 acgtcgccct cgtctacctc atccaccgca tcggtggtct caccgtcgag caatatcagg
      781 accattggct caaccagcac ggcccgctcg ccaaggcgct ggtgccgacc agtggctacg
      841 aacagaccca tgcagaccac gacggcggag cagcgttggc cagcaggctg ggcttcgcca
      901 caccggactt cgacggcaac gcgacatgct tctttgatac ggcgcaggac ttcgccgaca
      961 tgctcactgc cagggagagc agtcgcgaca tgaacgtcat ctacgaggac gagctgagat
     1021 tcctcgacca cgatcgctcg ctcggtgcgc tgatgcgcca ggtgccgctc ggcgctgctc
     1081 catgacgtac tgccactgcg atatcagtgc ctcggtggcg acgctcacgc tggactcacc
     1141 gggcaaccgc aacgcactcg gcatgcaact gaccaccgag ctgcgcgatc acctcaccac
     1201 ctgcttgcgc gacgcagccg tgcgggtgat cgtcatcacg gccacgggta cggtcttctc
     1261 gtcgggatgg gatctcgatc aggagccgtc gcagggtctg ctcgtcgacg acgtcctggt
     1321 cgaggtgttg gaggcgatcc gcaccggccc caagacggtg gtagccaaga tcggcggtca
     1381 tgcnnnnnnn nnngtcggag gggcgctggg cctggtcgcc gcgtgtgatc tctcgatcgc
     1441 cgcggccgac gttcgcttcg gcttcggcga ggtgcgcctc ggactcgctc cgaccaccgc
     1501 cgcggtccac tgcctgccca agatgcgccg cgccgacgcg ctcgagctgc tgctcaccgg
     1561 tgagcggttc ggcgcccagc gtgccgccga ggccggcatc atcaaccgcg tcgtcgcccc
     1621 cgagtcgctc gacgactccg tcgccgggct cgtcgccgcg ttgatcctgg gtgggccgat
     1681 cgcgctcggc gcatgcaagc gcttggcacg cacgatcacc gatgccggac cggacgcata
     1741 cgccctggca cgagagctga acgctgccct cgtcggttca ccagaagcga ccgaagggat
     1801 cgccgccttc ctcgaccgcc gcccacccgt ctgggcgcgg acctgacgag gccgggtcag
     1861 tcgaccagcg tctccagcgc ggacaggtcg acaacaggtt gacgctgctg acccggttcg
     1921 agacggcgcg aacccggctc gacgtcgagc atgctctgca agccgagtgc cttgctgtgg
     1981 gcgcggatct ccggaaggac ttcgttggcg atcagccgca tgttctcccg ccgtcgctcg
     2041 ttcgagcagt tgccctggac gttcatgaac accatcaagc ccggacgcaa cacgctcacc
     2101 atctgcttga tcttggggat gacctgatcg ggggtcccgg caacgatctg catgttcctg
     2161 acgtgccggt ggaagctttc gtcgatgcgg ctcttggcct tggccatgtc gaactcaccg
     2221 cgcccggtgt cgctgccatt gagcttctcg cgactgacgc ccagccatcc actgctcggc
     2281 atctttgcca gtcgcttgat cgctgccggc gaattgaacc cggctggaag cgtgtattca
     2341 ggcctcgcga acgagccgtt gccgttcgca tagagatgat aacgagcaac ctcgatggcc
     2401 tcttcctccg tcgcggccac cgatgccatc gtcaggtagg cgaagttctc cgagccggcc
     2461 tgatagccac attcggcagc tcgatcggca tagctgttcc acagctctgc agtcggaccc
     2521 aacgaggtgc cgaggccgag atacggatac gcatgctcgg cggcccactc gatcgtctcc
     2581 aggctgagca caccgggaat gacggtcggc ggacgcggct tttggtaagg caggacccat
     2641 ggattgacgt ggcggtagtg gaagtggttg ccctcgtagc gccatgggcc aggccgcgtc
     2701 catgcctgct cgacgaaggc caaggcctct tcgaacatct ctcggttgtg ggccgggttg
     2761 gtgttgttga agaactgctc ggggcccgac ccccgcaccc agccggcgat cagccgacca
     2821 cgagagatca ggtcgatctc ggccagctcc tcggccattc gcagggggtt gcggtgcgcc
     2881 ggcaacgggt tgccgatcag cagcaggcgg acgcgctcgg tgatgcgggc gagaatcgct
     2941 gcttcgaggt tcatcgtgct gcccatgcaa aacggattgc cgtgatgctc gttgagggcg
     3001 acggcatcga agccgagctc ttcggcgtag cagttctcgt cgaggtagta gttgtagtca
     3061 cggctggcga tcgcgggatc gaactttgag ttcggcatgc tgaagtagga gccgtgctga
     3121 aggatctcct cctcgctcaa accgcgatag ggacgctcgg tgaagtaacc ggccagcatg
     3181 atgtagtcct ctcagtggtc gcggggcagc aagaacttgg agacgcagcc gacgaatccg
     3241 tcgacgtctt cgatctccgg tcggtgcccg acagcaggca ggatgtgcac ctcgacgttg
     3301 ggcagtgctt cttcgtacgc gtcgatggct ccacgaggga cgatgaggtc gtcctcgccc
     3361 cacaccacca aggtcggcag atcgcagagg ccctggaggt ggtgaggcag gctcgggttg
     3421 aacatgaagg gctcccagcc caaccgtgac gtctccccgc gggcggcctc gaacagctcg
     3481 aactgctcag cggtcatgtc cccgccgtag atctgcccga actcctcgac ctcgtgatgg
     3541 ctcaccgtcg ccgcgacgtg agtcatcatc gtcgaggcca ggaagtcgaa gatctcaccc
     3601 gactgcggcc gcactccgag cggagcaacg aggaccatcc gctcgaacag atccggcgcc
     3661 atcaccgcca tctccgcggc gatgaaacct ccagcggaga agccgatcac atcggtcggg
     3721 ccgagctcga gttggcggag caggcgaccg tagaaagtgg cgaggtcgcg gaagtcctgc
     3781 aaccacggga tccgtggggt gtgtccgaac cctggctgga gcgggatgac gaactcgcac
     3841 tgctcggcga gctgctcgtt ccagcgcatc cagcccggaa atcccaactc gtcgtggagc
     3901 atcagcagcg gtctgcccgc tccaccacgc agcaccgtga tcggcgttcc accgacgtcg
     3961 acgatcgatt ctgtcaactc ggccatcagg agcgctccgg ttgggcgagc aggcacggtg
     4021 tcaggttgcg gtcgtgagga tcgaagccac cgctgcggtc atcgactgct accaccatgt
     4081 tcgctcccag ttcgttatct accatacgtt tggtagttga tcatagagcc acgcccctgc
     4141 tccgtcaacg tgggcctcgg gctcgtccgt cgcaaaccgc cctgacgacg ggccggctgc
     4201 gatcagtagc tggccgcagg cacatcgacg cgggcggtac ggatatgcga gaccgacttg
     4261 ccggccatcc actcgtccaa cgtcgtctca gccgacacca tgaacagcgt tcggccgtcg
     4321 tctccaccga gcgcgcagct cggggtccac tgtccgtcga tggcgatgcg atgggtgatc
     4381 tcgccaccgt cgaggacgcg gaggtagtcg caacccgtcg atcccgagac ccacacggcg
     4441 ccttcggcat cgatcgccag accgtcgggt gcgatcccag gtagatgcgc gaaggttcgc
     4501 ctgcccgaga ggctcccgtc gtccgccacg tcatacgcgg tcagctcctc accgaagagt
     4561 tgagcaacga tcaaggtccg cccgtcagga ctgagcacca tgccgtcggg gaagatcaga
     4621 tcccccaccg cgacgaccgc tggttcgccc ggacgaacga ggatcagctc gccaggcttg
     4681 ggctcttcgc cagaacgcag gtcgtagccg aagttggaca cgtaggcgcg cccctggccg
     4741 tcgaccagca tgtcattgca ctcggaacgc gacgcgacgg acatgtcggc gtacaacgac
     4801 acctcacccc cggcgatcga acgcagctgg cgggatttca tcgccaccac gatcggagca
     4861 ccactgggca ggaagccgag ccccgacggc tcgtcgtcag gcaacgtggc ttgtagatcg
     4921 aggcgatcgc cgtcgatcgt gtagaccgcc cggccgaaca gatcggacag ccacagcttg
     4981 ccgtcgtgcc agcgcagccc ttcggcgtag tgcagcttgc ccatgacaag tggtcggact
     5041 tcgaatgtgc cagtcaacgg tgcctcctca agctgcacgc cgacgggttg gtgccatcaa
     5101 tattcgacca accgacggtt tgagaaaaca tacctaccat gtagagggcc gtcaattgca
     5161 gtccgggctt gggtgagctt gactgtccac gtgcgtccga gcaagatggc tcaaccaact
     5221 gattggttta agtgaggagt tgtatgtctg accggggaga cgcagcaacc atgggaacgc
     5281 ctggcgtcca gttcggagac cagttcttcg ctcaggaaga gatcttcgac cgaggtgcac
     5341 gcgctgctcg cggactggct tcgatcggcg tacgacgcgg cgacgtcatc gccacctacc
     5401 tgcgcaacga catcgcgttc ttcgagaccg cgatcgcggc agccacactt ggcgtgcaca
     5461 tcgtgccgat caactggcac ttcaagaccg acgagatgcg ttacgtgctc gacgactgcg
     5521 gtgctcgggt gctcgtcgcc cacaccgatc tgctcgctgc gctgcacggc gacctcccgg
     5581 cggcggtgac catcttctcg gttcccacga tcgtcccccg tcgtcacggc gcgccggccc
     5641 ccgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngttcgagc cgtggagcga
     5701 cccgccacaa acgcccgagt tcagcatgaa ctacacctcg ggtaccacgg gaaagcccaa
     5761 aggtgtccgc cgcgaaccga tgaccgacga gggacgggcc gcgatggctg ccatgttcgc
     5821 ccaactcgaa gtgaaatccg gcatgcgcac gatcgtggcg gcaccgctgt accacgcggc
     5881 accgttcggc ttctcgatgg ccgtgctgcg tgccggaggc ctgatgatcc tgatggaccg
     5941 cttcgatgcg gaggagtacc tcgccaacat cgagcgctac aaggtcgaca acatccagat
     6001 ggtcccgacc atgttcgtcc gcttgctcaa acttcccgcc gaggtccgcg agcgctacga
     6061 cgtgagctcg ctgcaactcg tcacccacgg cgcggcaccg tgcccggccg aggtcaagac
     6121 cgcgatgatc cagtggctgg ggccgatcat caaggagttc tacgcagcca gcgagttggg
     6181 catgatcgcg acctactgca gctcggagga ggccctgtct caccccggca ccgtcggtcg
     6241 gcctgtggac ggcgccgaga tccgcatcct cgacgatgcc ggcaacgagt tgcccagcgg
     6301 agaggttggc gagatcgcga tgtggatccg cggcacgagc gccttcactt accatggcaa
     6361 cgaggagaag cgacagggca tccagcgcgg caacctggtg acctgcggcg acgtcggcta
     6421 cctcgatgcc gacggctacc tctacctcac cgaccgcaag agcgacatgg cgatcgtcgg
     6481 cggtgccaac gtgtatccgg ccgaggtcga gcaactgctc gtttcgatgc cgggcgttct
     6541 cgactgcgcg gtcttcggca ttcccgacga cgacctcggc gaagtgctcg ccgctgtggt
     6601 tcagcgccag cctggtgcga ccatcaccag cgacgacgtg atcggctggt tgacgccgcg
     6661 aatcgcgcgc atgaaggttc cggccacggt cgagtttcgc gacgagttgc cccgcgagga
     6721 cagcggcaag ctgttcaagc ggcggctcaa ggaggagttc gccgcccgcc gctgacgacg
     6781 ctgcgccacc gggtgctcag cgggtgtact cgcccgcgtt caccaacagg cattggccgg
     6841 tgatcgcctt ggcccgagct gacgccagaa aggctgccgc ctcggcgacg tcgccatcct
     6901 ccgcgacggt gcccaacggc agcttgcggc cgatcttctc gatgagcgca gccccgccgc
     6961 caccggtcgc ggcgtcgagg ctcttctcca acgacggccc ccacatccat ccgggggcga
     7021 cgacgttgac ccggatgccg cgcggtccga gctcggccgc gaggtggtga gtcacgccga
     7081 ccagcgctgc cttcgacgcc gcgtatccgg cctggatcac attggggaag taggccgcct
     7141 gcgagccgat gaagacgatc gaaccctgcg tctccatcgc cggcacggcg gccgacacga
     7201 cctgcatcgc gccgaacagg ttgacctcga agaggtcacg ccactcgtcg agatcggcgt
     7261 ccgcgatccc gccgaccagc gcgtcaccgg caaccgcaca gatcaccacc gcgtcgagcc
     7321 caccgaaccg ctcgaccgcg ctggtgacca gcgccctgca ctgctcgctg tcgcgaacgt
     7381 cggtggtgcg ccacatgaca cggtccccgc tggggtcgac gccgaatgcg atgtcctcca
     7441 gcaccgacgc ggtccgtgcg ccgagcacga cgttgccacc ctcacgacgc acgactcggg
     7501 cgatctcctg accgagcccg gtcccggcac cgacgacggc caccgtcttg gcgtccaaca
     7561 tccccgcgct cacgacgatc ggacctcgac cccgaacact cgggctgcgt tctcgtggag
     7621 gaacttcggc cacacgtggt cgcgcagcgg tacctgttcg agctgggcga agacgtcgtc
     7681 gtatgcgagg ttcggccagt agccggcgaa cagcacccgg tcggcaccgc gcgtgttggc
     7741 gtactggatg atctccggtg cgtagtactt gggagcaaag gccgacgtcg cgaagtacag
     7801 gttgggccac ttgagcatca gcttgacgca catgtccgcc cacggttctc cgccgtgctt
     7861 catcacgatc gtgagatcgg ggaagaacca cagcacctcg tcgaggtacg cagggttctg
     7921 gcagtcaccg agcacgcgcg ggcctgggat gccgacgttc atgtacaccg gcagaccgag
     7981 ctcgatgcac ttggcgtaga tcgggtagta ctccttcgag ttcggtggga tcggcggcca
     8041 caacatatgc ggcgagagcg acacggttct gatctgcggg tagtcgcgta ccagttgctc
     8101 gagcttgcgg atgccgcgca tgccttcgtg cgggttggca cgaacgccga cgatgaaccg
     8161 atccgcccac ggccggatcg catccatgac caccggcacg tcgtccatcg ccaccaacgt
     8221 caggcccttg gcgatgttga accggtcgta ctgctcgacg accttgccgg gctcgttgga
     8281 gagccgggat cgttcctgag cccctttgaa gaggtagttg acgatgcccg tcgacggcca
     8341 cgcggcgagg ctctcctcgt cacggaacgc ttctcgcggg atgggcatcg atgcgccgac
     8401 accgttgggc cagaccatcg acagacagtc gaggattggc agatcgacgt cacgcagtcc
     8461 cacgttgcac tctccttctc gccgtgaccg gcgcacgttc gctcgtcgcc cactagtcta
     8521 ctaccaacca atcaactggt tgatgagagg gatggccgat gggtgcagag atcatcgttg
     8581 agaccgacgt cgcggtcccg atgcgggacg ggacggtgct gcgcgcggat gtctaccgcc
     8641 ctgctgaagg accagcaccg acgctgctcg aacgcacgcc gtactcgaag gaccagttgg
     8701 tcgggacgat cttcgtcatg aaccccatgc gcgccgcccg ggccggatac tcggtcgtcg
     8761 tgcaggacgt gcgcggccgg ttcagctcgg acggagactt ctaccccttc gtcaacgaat
     8821 cgctcgacgg ctacgacacc gtcgagtggt gcgcggcgca accatggtcg accggcaaag
     8881 tcggcatgtt cggcagctcg tacatggccg ctgcccaatg gcaagcggcc aaggccaacc
     8941 cgccgcacct gacgaccatc acgccattcc aggccagcgc cgactaccgc gacgggcgca
     9001 gctaccgcgg cggcgcgttc gaactcggct cgctgctgtc gatcgccctg tacgcgctcg
     9061 gtgccgggca gatggcccgg ctgccgctgg ccgaacgacg ccccatgtgg gacacgttgc
     9121 ggggggcgat cgacgacatc tcggccacct cgcgggccgt gctcgagaac cggctggccg
     9181 gtaccgtcat cccggagatc attccgtatt tcttcgactg gttggatcac aacgagcccg
     9241 ggccctactg ggatcgcatc gacgtcaccc agcattacca gaacatcgat ctccccgtcc
     9301 tgcacgtgtc gtgctggttc gaccaattcc tcgtgggcac catccggaac ttcgtcggca
     9361 tgcgcgaacg cgctgccagc cagcacgccc gcgacaacca acacctgttc atcggcccgt
     9421 ggggccacta cgcaccgcgc accgctctgc tcggcaccgc tcgcatcggc gatatggacc
     9481 tcggcgtctc ggcggtgagc gacctcgacg cgatgctgat cagctggttc gaccgatgga
     9541 tgaaggacga gccaatcgca gggcgggcca tcgctcccgt gcgcctcttc gtcaccggct
     9601 ccaacacgtg gcacaacctg agccaatggc ctccggagac gacggcgacg ctggacctgt
     9661 tcatgcacag cgaaggatcg gcgaacagca gcgacggcga cggccggctc gacagcaccc
     9721 cgccgacggc cgacgaagct gccgacacct acctccacga ccccgccgac cccgttccga
     9781 cgtgtggtgg tgcacacctg gtgctcgaag cgacgtatcc gcaaggcgcc gtcgatcagc
     9841 gcgacgtcga ggcgcgcgac gacgtcctgg tgttcaccac ccacgcgctg gacgcagacg
     9901 tcgaggtgct cggcccggtc accgctcacc tcaccgtcga gtcgaccgcg ccatcggccg
     9961 acttcgacgt caccgtctcg gacgtccacc ctgacggccg ttcgttgaag atctgcgacg
    10021 ggatcctgcg gaccatcctc gacgacggtc cggccgcgat cgtcgtcgat ctcggcgtca
    10081 tcggccatcg attcctcgcc ggacaccgga tccgggtcca cgtggcgagc agcaactttc
    10141 cccgctacga cctcaatccc gccgcggtca cggccacgca gtccctgcac cacagcgcgg
    10201 cccatacgtc gttgctgcgc ctcccgatct cggcgctgtg accgggtccg atcacaccgc
    10261 cggtacagcc ccaaagagcg actcgaacca ctgacgatcg gccgcgacca tctcgatccg
    10321 ctcgcgcgga gtggccttca ccagcacctc atgctcgaac catccctcgt agccggcggc
    10381 caggaaatcg tggacgaggt cggcgaccgg tactgcgctg gcggcgccgc attgcgcacg
    10441 cacgtaacgc cgatcggtca tctcgtcggc acgaacgtcg gtgatctgca ccgcgaacac
    10501 cagctcgagg ttggctgcga cgccggcgac gagatcacgc tcccaccatg acgcaccggt
    10561 gtcgaccacg acgccggttc ccggcacctg cttggccagg tccagggcgt ggctgagggt
    10621 gtggacgtag gagagctcgc gggcgatggg gtgcagcggc tcgatcatcg gactgacgcc
    10681 gctgcccgcg acccggtcag cgacagccga cagagcatcg acgagctgct ggtcggcagc
    10741 ggccggatcg cgtccggcga tgtcgcccgc aatgaagaga aagcacggcg caccgagctc
    10801 gcgcgcgtcg ccgaacgacc gttccagcgc atccagatcg ggggccacag gacccaagcg
    10861 catcagggcc gggtcgatgt agctcgacac ggccagtccg gagtcggcga tgatctgctt
    10921 cgcctcggca atgccgatgc cgccgaagtc ggccgcgttg aggctgatcg cgctggcgcc
    10981 gccgccgact gccgcttcga cgtcacgagc gaggtcaccc aggcggagta cgtgttggca
    11041 cacggagaac cgccgtggcg ctggcaggcc ggtcaccggt ctcgtcaagg tcacagtgca
    11101 catggtatct acctatcgtt tggtatgcat ctccgcgcca ttgcgcgaag atgcttccac
    11161 gatgcacgcc gtcgacagcg acactcactt ctacgaatgc ctcgacgtgt gggccacgtt
    11221 cgccggcgct tcgtggcgcg accgaatgcc gcagctcgtc ggcgacggat cgcgcctgct
    11281 cctccaggtc gacggcacgg cctaccccnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11521 nnnnnnnnnn nnnnnnatac gccgcggcgt tcgccagcgc cgatccacga cgtctgcgtt
    11581 gcgcgatgat cgccccgctc aaccaccccg tccaggccgc agcagagatc gccttcgccc
    11641 acgaccaact cgggatgacc gtgctgctcg ccagtttcac cgcacctggc gcgcgctcgc
    11701 tgagcagccc tgatctcgac gtggtgtggg ccgcggccca cgagcgggcg atcaccgtca
    11761 cgttccaaga ttcctcgctg tcggcagtgc cgacaacgtc cgggatcgcc cgcgcgaaga
    11821 cgtggcgcat gctctacctc gcgacccaca tcgtcgaagc ccaactcggt ctggccgatc
    11881 tcatcctcgg tggcgtgccc gatcggttcc cggcgctgcg gttcgggatg caagagacac
    11941 acctcacctg gctaccgtca tggctgcagt tactcgacga gcgctttggt catcggcgac
    12001 aaccctcggt gcgacccagc gacgcctttc gccgccagtt cttcgcgtcc gggtttcccg
    12061 acgagcccgg cgtacgtcgc tttatcgacg aagtgggggg acgcaatctg gtctttgcca
    12121 gcgactggcc gcaccgcgag ctcatgccag gcaccgaccc aaactggaca gcagaagtgc
    12181 tccggcgcaa cgacctgagt gccgccgagg ccgtcgcagc gctggagaca aaccccaacc
    12241 attggttttc caactgacca ggctcgctta cgatatagag ccatgcctcg ttccgatcct
    12301 ccatccacct cacgcgccaa gaaggcgtcg gcgaatcccc ggaccaaatc ggtagcggcc
    12361 aagcaaccat cttccaagac tgtgaccaag cgcacaccag cgctcaagca tcagggccgc
    12421 aacagcgccc ggcgcaacga gatcgtccgt gccgcggccg agaccttcta ctcgaaggga
    12481 tacgacgcga cgacgacaca agacatcgcc gaagccgtcg ggatgctcaa aggcagcctg
    12541 tactactaca tc
//
LOCUS       DAORNF010000007        12075 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_3987_length_12075_cov_1.841930, whole genome shotgun sequence.
ACCESSION   DAORNF010000007 DAORNF010000000
VERSION     DAORNF010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 12075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12075
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3987_length_12075_cov_1.841930"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..727
                     /locus_tag="PLV68_00625"
     CDS             <1..727
                     /locus_tag="PLV68_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99766.1"
                     /translation="MGIAMATGTTTIALRSAHEAVNVLGLVGLVIGSTMPYFAATVGR
                     SKMSPLARRGVLTGVIAAMASGIAITAASLAADAPTIAAAGLMVYALSIGGVVRVLPS
                     LTRRQMRWAGPRLLALWTGAAWWIVGVVAVSADLAAGDPPFAQRWLLVIVLGGYVQIL
                     WGSLAYLLPMLRGGGPDHLSAGFATTRSWIGFGAANLTALAIATALPRSVSYATGAVW
                     ALDTAWRLGRVGTRRADRPQSGA"
     gene            complement(777..1436)
                     /locus_tag="PLV68_00630"
     CDS             complement(777..1436)
                     /locus_tag="PLV68_00630"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRD99767.1"
                     /translation="MSDYRSLGASPAVYEYLVAHSSPPDPVYAAISTETAERTGRAAG
                     MQIGPDQFVFMRLLTQLLGARLAVEIGTFTGSSAAAIAGGLAPGGRLICCDVSEEWTA
                     IARRQWQAAGLADRIDLRIAPAIETVQSLPDEPIDLAFVDADKTSYVDYYEALVPRLR
                     PGGVLLADNVLWSGQVADPSVTDDSTVAIRAFNQHVAADDRVEAVVLSIGDGVTLARR
                     R"
     gene            1469..>1664
                     /locus_tag="PLV68_00635"
     CDS             1469..>1664
                     /locus_tag="PLV68_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99768.1"
                     /translation="MTTTPITTPITTPTPHALVTAAAATAAGGHLAAEAARFAALGWM
                     RGTSGNLSTVLQRDPLHLAIT"
     assembly_gap    1665..1688
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1689..2118
                     /locus_tag="PLV68_00640"
     CDS             <1689..2118
                     /locus_tag="PLV68_00640"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HRD99769.1"
                     /translation="VVVDAAGEPVAVDGLAVQRPSAEAGLHARIAAVTGAGAVVHVHA
                     LAAVVAARRWPEGVVLHDVEMLKGIGRGAHDDVVTVPVVANSQDMAELGDRFVARYDS
                     GVPAVIVADHGMYVWGTDLRQARHHTECLEWLLSLAVASA"
     gene            complement(2138..>2547)
                     /locus_tag="PLV68_00645"
     CDS             complement(2138..>2547)
                     /locus_tag="PLV68_00645"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRD99770.1"
                     /translation="DGPASHDRLDPFEEMRSAIRHARIDQLDASFLDARTVLGMATRE
                     AGLALGRTDLGHLQAGARADMIALDLDGPGFSPVLDPDDLPGRVVWAGSPAAVRSVWV
                     EGALVVDEHRCLTVDRAEASRQVTERARGLVSS"
     assembly_gap    2548..2600
                     /estimated_length=53
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2601..>2934)
                     /locus_tag="PLV68_00650"
     CDS             complement(<2601..>2934)
                     /locus_tag="PLV68_00650"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRD99771.1"
                     /translation="PLIEIGVGPHSAYALSRDALLQVKQLVDDEPMLVHIHVAEQPDE
                     GDGVLAEPGLTVPAYLDGLGLLSPRALAAHCVWMTDDDIDRFATNHVGVAHCPASNGR
                     HASGAARIR"
     assembly_gap    2935..2967
                     /estimated_length=33
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2968..3478)
                     /locus_tag="PLV68_00655"
     CDS             complement(<2968..3478)
                     /locus_tag="PLV68_00655"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRD99772.1"
                     /translation="MTVLATGLGTRLLADHLITMEPGVPVHSPGIVDVLDGRVTWSGP
                     AAAAPPSDAKVQTVSGLLMPGLISAHAHTPMVLLRGTGEGLATIAWLHDVMWPRESRL
                     TADDVEWAMRLGASELLLHGVTTSSEMYFWPAAIARAAADAGLRCLVAAPLLESPGFA
                     HHGTIAEQID"
     gene            complement(3475..4395)
                     /locus_tag="PLV68_00660"
     CDS             complement(3475..4395)
                     /locus_tag="PLV68_00660"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRD99773.1"
                     /translation="MFDQLFDAALLDSVVRATVPILIAALGGLICERAGVFQISLEGT
                     MLVGAFTAVSVSYASGNAYLGVLAAIVAGVAVSQILAYGCITRRGDPIVIGIAINLLA
                     VGLTGFLLPQFFDVRGVFKSADIAGLHRYAIPLLSDIPVVGKSLFRMTIPAYLAFLLV
                     PVIWVMLFRTPLGLRLRGVGERPHAAATRGVPVTGYQYAAVATSGALAGLAGAQLAIG
                     NVVQFAEGMSSGRGWIAVVAVLLGGAHPYGVLAACSLFGLAEGLGFRLQGNGLAVQIT
                     DALPWIVTLVALLLARKQFARLIDLTGRTS"
     gene            complement(4395..>5095)
                     /locus_tag="PLV68_00665"
     CDS             complement(4395..>5095)
                     /locus_tag="PLV68_00665"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99774.1"
                     /translation="LWALIPALAQTELQLPILISGLLLNQPARALTSYLTKNYFADPA
                     ASVTSMRALPKDVRVPAVGWVTGASATLFLILALVVTISLFNNRTAPGYQNYVSGLNL
                     RFSRYGGVAVRRQTIVSMAVGGAIAGVVGAHLVLGQAFRFVDGDLPGTGFAWTGLLVC
                     LLAANRAYPILIAGAFFSALQVGGLAMQRNAGVSSQLAQVLQALVILALALQITFVRR
                     RRRANVITAERAGA"
     assembly_gap    5096..5105
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5106..5488)
                     /locus_tag="PLV68_00670"
     CDS             complement(<5106..5488)
                     /locus_tag="PLV68_00670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99775.1"
                     /translation="MADTALMPAARQRLFALAASPVLASLVLAFVVSGAILALTGANP
                     FSAYSEMWSGAFTGNGPRTLINRAIPIVGMALATSIPFRTGIIHIGAEGSMVVGGFTA
                     SVVALELHGPGAVVLWVSFLAGAAAG"
     gene            complement(5481..>6490)
                     /locus_tag="PLV68_00675"
     CDS             complement(5481..>6490)
                     /locus_tag="PLV68_00675"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRD99776.1"
                     /translation="QERDSLFDVLRTLRAAGRTIVFITHKLNEVMSLSDRVTVLRRGR
                     VVDEALTAGTSPAALSLAMTGRSVDTGKRPHEHEPGAPLLEVRDLDVTDDDGLPRVAG
                     VSFSVAAGEVVGIAGVTGSGQSELVEALIALRAVAGGQITLAGHVLDDHGVRARRDRG
                     IAYVPEDRHAVGSAGPASTSDNLLMGYQWRRRFQRGGWLKRRPVRRHAADLVEQFDIK
                     VSGIDQPVGQLSGGNLQKVVVAREFGHDAPLLIVEQPTRGVDIGAIEFIHGRIIEHRD
                     ADRGILLVSSELWEILALSTRILVMFEGRIVAELDPRLTTDVEIGLAMTGALQHEHAH
                     G"
     assembly_gap    6491..6507
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6508..7015)
                     /locus_tag="PLV68_00680"
     CDS             complement(<6508..7015)
                     /locus_tag="PLV68_00680"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRD99777.1"
                     /translation="MSVELQLEQVSKRFGDVVANDRIDLSVHAGEIHAIVGENGAGKS
                     TLMSILFGLVTPDDGSIVVRGEPVQFRSALDAIAAGLGMVHQSFQLFPTMTVAENVVY
                     GHEPRKGWFLDRAAATERVAALAAEFGLGVDPTARVEQLGVGEMQRVEILKALYRDAQ
                     VLILDEPTA"
     gene            complement(7035..8135)
                     /locus_tag="PLV68_00685"
     CDS             complement(7035..8135)
                     /locus_tag="PLV68_00685"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HRD99778.1"
                     /translation="MNSNNTRRLRSAVGAVALLGVLASACGSDEKKSEQTTTTTAAAG
                     GDSTTTAASTGDGPSFIYITPTNIGPNKFLELGKIGTEAAAARLGGTSKTFESTDLNS
                     RRANLEAAVEEKPDIIILTTFDFTDLSAEFAAANPTQQFILIDQCPAEPPANLHCGVF
                     RENEMSYLLGVIAGSLSESGKVGSVVALDIPFLHRFTDNFALGAQSVNADITDSQVFI
                     AGDNPFADPATAKEQALALHAQGVDQIFAAASGSNGGVFEAAQEEDFLAYGTDVNQCA
                     LAPGHIVDNALKLVDVLTEQLIDQVLAGTAGSVVSFGLAEGGVGVVALQDDVASSGCL
                     IADHPDVIAAVKTARDAIVAGTIVVPDPVTGG"
     assembly_gap    8211..8229
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8230..>8446)
                     /locus_tag="PLV68_00690"
     CDS             complement(<8230..>8446)
                     /locus_tag="PLV68_00690"
                     /inference="COORDINATES: protein motif:HMM:NF013197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99779.1"
                     /translation="FVVVAPESTVDLATASGDEIEIEDRGDDEVCSFRGAPVSPPGTR
                     TINPAFDVTPVALISALVTDRRVVQFRT"
     assembly_gap    8447..8579
                     /estimated_length=133
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8580..9270)
                     /locus_tag="PLV68_00695"
     CDS             complement(<8580..9270)
                     /locus_tag="PLV68_00695"
                     /inference="COORDINATES: protein motif:HMM:NF013197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99780.1"
                     /translation="MLSDNTPLDPAGLDPAGVDTADRVPTVAWVDGAIELIDQTRLPS
                     EEVVVRCASIDALSDAIVRLVVRGAPAIGVAGAYGVALAASLHDPAVQPDEFAAAVAA
                     VRNARPTAVNLAKMVDRTAALAEKGFDVLLAEADAIRDEELAASTAMGELGADLLLEL
                     AGDRPLRVMTICNTGGLATVERGTALAVIQTLHERGKLTEAIPLETRPLLQGGRLTTW
                     ELARMGAPHRLI"
     gene            complement(9332..10075)
                     /locus_tag="PLV68_00700"
     CDS             complement(9332..10075)
                     /locus_tag="PLV68_00700"
                     /inference="COORDINATES: protein motif:HMM:NF019322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRD99781.1"
                     /translation="MTTDEPLAAAGARGTNLSERLADSLRVRLEAGEWRPSERLPTEH
                     ELAAAYGVSRATVRTALRALDSRGLIVTRRGLGTFAAAVTSAVSANLQRLESMSETIR
                     RLGRKPGSNFRSIQIRAATDCEIEALLLQPGTSVLATHREITADDDVVAYSHEVLPVD
                     LLGADFDIRSVAGSMFGVMDAHGVVAASALTNLHASRGEEIGWGEHPADALFLLLEQT
                     HFDTTGRAVVFSRTWFIEGRFQFTLIRVR"
     gene            10145..10885
                     /locus_tag="PLV68_00705"
     CDS             10145..10885
                     /locus_tag="PLV68_00705"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRD99782.1"
                     /translation="MITIDLSDTVVAVTGASGGIGAAIAARFTDAGAATVVHHRREPV
                     PQVGPLSVAVSIDLATDDGPQRLIDAAVATFGRLDAVINCAGVQPVVDFASMTTPEWD
                     EMIAVNLTAVHRVTQAFARHAAERGGGGAVVHVASIEGHQPAPGHSHYAVAKAGVLMH
                     AKAAAIELGSIGVRVNTVSPGLVHRDGIEHAWPDGVARWRAAAPLGRLGAPSEIGDAC
                     VFLCSPLAAWVTGTDLVVDGGVSARPTW"
     gene            10879..>12075
                     /locus_tag="PLV68_00710"
     CDS             10879..>12075
                     /locus_tag="PLV68_00710"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HRD99783.1"
                     /translation="MVNEATVRTVPTGDGPVGDELDQCVHASRLLGADPTLVLHGGGN
                     TSVKMPWRDITGAVIEALYVKGSGWDLAIIERAGFAPLPLARLRALLALETLSDPDMM
                     RELSAARLDPAAPQPSVEALLHAFLPHRVVLHSHADLILTLTNTPDGDAIVREVFGDS
                     VVVVPYVMPGFDLAREVLARWDADSHPGTVGIVLMNHGLFSFADTAEAAYRSHLDLID
                     LARQRVAASPPLAHRDPAVAANAHACACQLAALRQTISAAAGQPMIVTRDTSADTMTF
                     VGRHDLADVATRGPVTPDHVIRTKRVPLVGRDVDAFVADYRSYYDEHEHRSRTPITML
                     DPAPRVLLDPELGMLTVGHRPADAAIAADIYRHTMQVITTSEERLGGWAPLPPFHLFD
                     LEYWDLE"
BASE COUNT         1872 a         4115 c         4056 g         1743 t
ORIGIN      
        1 catgggcatc gccatggcca ccgggacgac gacgatcgca ctgcgctcgg cccacgaggc
       61 ggtcaacgtg ttggggctcg tcggtctcgt catcggttcg accatgccgt acttcgcggc
      121 gacggtgggg aggtcgaaga tgtcaccgct cgcccggcgc ggtgtgctca cgggggtgat
      181 cgccgcgatg gccagcggca tcgccatcac ggcggcatcg ctggccgctg acgcgccgac
      241 gatcgccgcc gccggtctga tggtctacgc gctgagcatc ggcggggtcg tgcgcgtcct
      301 gcccagcctg acccgtcgcc agatgcgctg ggccggacca cgtctgctcg cactgtggac
      361 gggcgcggcg tggtggatcg tcggcgtcgt ggccgtcagc gccgacctcg ccgccggaga
      421 tccgccgttc gcccagcggt ggctgctcgt gatcgtgctc ggcggctacg tccaaatcct
      481 gtggggttcg cttgcttacc tcttgccgat gctgcgcggt ggtggtcccg accatctgtc
      541 ggccggtttc gccaccaccc gctcgtggat cggcttcggc gccgccaacc tgaccgcgtt
      601 ggccatcgcc accgcactcc ctcgctctgt gtcgtatgcg accggggcgg tctgggcact
      661 cgacacggct tggcgcctcg gccgcgtggg tactcgccgg gccgaccgtc cccaatcagg
      721 ggcctgaggg caccggtggg gagctggtcg acagggtttg acggcgccca gtccggtcag
      781 cggcggcggg ccagggtgac cccatcgccg atcgacaaga cgacggcttc gacgcggtcg
      841 tccgcggcga cgtgctggtt gaaggcccgg atcgccacgg tgctgtcgtc ggtgaccgac
      901 gggtcggcca cctggccgct ccacaacacg ttgtcggcga gcagcacacc gcccggccgt
      961 aggcgaggaa cgagcgcctc gtagtagtcg acgtagctgg tcttgtccgc gtcgacgaag
     1021 gccaggtcga tcggctcgtc gggcagcgac tgaaccgtct cgatcgccgg agcgatgcgc
     1081 aggtcgatgc gatcggccag ccctgctgcc tgccactgcc gccgggcgat cgcggtccac
     1141 tcctcgctca cgtcgcagca gatcaagcgc ccgccagggg ccaggccacc cgcgatcgcc
     1201 gcggcggacg atccggtgaa ggtgccgatc tcgaccgcca ggcgcgcacc gagcagctgg
     1261 gtcaggagcc gcatgaacac gaactggtcg ggcccgatct gcatgcccgc tgcgcgtccg
     1321 gtgcgctcgg cggtctccgt gctgatcgcc gcgtacaccg ggtcgggcgg ggagctgtgg
     1381 gcaaccaggt actcatagac cgcaggagac gcaccaaggc ttcgatagtc agacatgacg
     1441 ccagggtagg gtcacagccg aggtttgcat gaccacgaca cccatcacga cacccatcac
     1501 gacacccacg ccgcacgcac tggtcacggc cgcggcggcg actgctgcag gtggccacct
     1561 ggcggccgag gcggctcgct tcgcggcgct gggctggatg cggggcacct cgggcaacct
     1621 gtccaccgtg ctgcagcgcg atccgttgca tctggccatc accgnnnnnn nnnnnnnnnn
     1681 nnnnnnnncg tggtcgtcga cgccgccggt gagccggtcg cggtcgacgg gctcgccgtg
     1741 cagcgaccgt cggccgaagc cggtctgcac gcgcgcatcg ccgcggtcac gggtgccggt
     1801 gcggtggttc acgtccacgc gctggcggcg gtggtcgccg ctcggcgctg gcccgagggg
     1861 gtcgtgctgc acgacgtcga gatgttgaag ggcatcggcc gtggcgccca cgatgacgtc
     1921 gtcaccgtgc cggtcgtggc caacagtcag gacatggccg aactcggtga tcgcttcgtc
     1981 gcccgctacg acagcggtgt gcccgcggtg atcgtcgccg atcacggcat gtacgtgtgg
     2041 ggcaccgacc tccgtcaggc ccggcaccac accgagtgcc tcgaatggct gttgtcgctg
     2101 gccgtcgcca gcgcgtgaca cgtcactgcg aacgcggtca cgacgaaacg aggccccgcg
     2161 cccgctcggt cacctgacgc gatgcttcgg cccgatcgac ggtgagacag cggtgctcgt
     2221 ccaccacgag cgcaccttcg acccacaccg aacgcaccgc ggccggtgac cccgcccaca
     2281 ccacgcggcc gggcagatcg tcggggtcga gcacgggtga aaagcccggg ccgtcgaggt
     2341 cgagggcgat catgtcggcc cgcgctcccg cctggaggtg tccgaggtcg gtgcgcccga
     2401 gcgcgagccc cgcttcccgc gtcgccatgc cgagcacggt gcgggcgtcg aggaacgacg
     2461 cgtcgagctg atcgatgcgc gcgtgccgaa tagcgctgcg catctcttcg aacgggtcga
     2521 gccggtcgtg cgacgcgggg ccgtcggnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn cgcggatgcg cgctgcaccg ctggcgtggc ggccgttcga
     2641 cgccggacag tgggcgacac cgacgtggtt ggtggcgaag cgatcgatgt cgtcgtcggt
     2701 catccacacg cagtgcgcgg ccaacgcgcg cggactcagc aagcccaacc cgtcgagata
     2761 ggcgggcacg gtcaggccgg gctcggccag cacgccatcg ccttcgtcgg gctgctcggc
     2821 gacgtggatg tgcacgagca tcggctcgtc gtctaccagt tgtttcactt gcaacagtgc
     2881 gtcacgcgac agtgcgtagg cgctgtgcgg accgacgccg atttcgatca gcggnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnncgt cgatctgctc ggcgatcgtg ccgtgatggg
     3001 caaaccccgg cgactccaac aacggagcgg ccaccaggca gcgcaggccg gcatcggccg
     3061 cggcgcgggc gatcgcggcc ggccagaaat acatctcgct gctggtggtc acaccgtgca
     3121 gcagcagctc ggacgcgccg aggcgcatcg cccactcgac atcgtcggcg gtcaatcgtg
     3181 attcgcgcgg ccacatcacg tcgtgcagcc aggcgatcgt cgccaacccc tcgcccgtgc
     3241 cgcgcagcag caccatcggc gtatgggcat gggccgagat caggccaggc atcaacaggc
     3301 cggacacggt ctgcaccttc gcgtcgctgg gcggcgccgc cgcggccgga cccgaccagg
     3361 tgacccggcc gtcgagcacg tcgacgatcc ccggcgagtg caccggcaca cccggctcca
     3421 tcgtgatcag gtgatcggcg agcaggcgtg tgcccaaccc ggtggcgagc acggtcatga
     3481 tgtcctcccc gtgaggtcga tgaggcgcgc gaactgcttg cgcgcaagca ggagtgcgac
     3541 gagggtgacg atccacggca aggcatcggt gatctgcacc gccagcccgt tgccctggag
     3601 tcggaagccg agtccctcgg ccaggccgaa cagcgagcag gcggcgagca caccgtacgg
     3661 atgggctccc ccgagcagca ccgcgaccac ggcgatccag ccccgccccg acgacatgcc
     3721 ttcggcgaac tggaccacgt tgccgatcgc cagttgggca ccggccaagc ccgccagcgc
     3781 acccgacgtc gccaccgccg cgtactggta gcccgtcacg gggacgccac gcgtggcagc
     3841 tgcgtgggga cgttcgccga caccgcgcag acgtaacccg agcggtgtgc ggaacagcat
     3901 cacccagatc accggcacga gcaggaaggc cagataggcc gggatggtca tgcggaacaa
     3961 gctcttgccg acgacgggga tgtcggacag caacgggatc gcgtagcggt gcaagccggc
     4021 gatgtccgcg ctcttgaaca caccgcgcac atcgaagaac tggggcagca ggaaacccgt
     4081 gaggccgacg gcgagcaggt tgatggcgat gccgatcacg atcgggtcgc cgcgccgggt
     4141 gatgcaccca taggcgagga tctgcgacac agcgaccccc gcgacgattg ccgcgagcac
     4201 gccgaggtag gcgttacccg atgcgtacga cacggacact gcggtgaacg caccgacgag
     4261 catcgtgcct tcgagcgaga tctggaagac gccggcccgc tcgcagatca gcccgcccag
     4321 cgcggcgatc aggatcggca cggtcgcgcg cacgaccgag tcgagcaatg cggcgtcgaa
     4381 gagctggtcg aacatcaggc acccgcccgc tcggcggtga tcacgttggc ccggcgccgg
     4441 cgacggacga aggtgatctg cagcgccagc gccaagatca ccagggcctg cagcacctgg
     4501 gccagttgcg acgacacgcc agcgttgcgc tgcatcgcca aaccaccgac ctgcaaggca
     4561 gagaagaacg ccccggcgat caagatcggg taggcccggt tggcggcgag caggcacacc
     4621 agcagcccgg tccatgcgaa gcccgtgccg ggcaggtcgc catcgacgaa gcggaaggcc
     4681 tggccaagca ccaggtgggc accgaccaca ccggcgatcg cgccaccgac ggccatgctg
     4741 acgatggtct ggcggcgcac ggccacgccg ccgtagcggg agaagcgcag gttcagcccc
     4801 gacacgtagt tctgatagcc gggtgccgtg cggttgttga acagcgagat cgtcacgacc
     4861 agcgcaagga tcaggaacag cgtcgcgctc gcacccgtga cccagccgac ggcagggaca
     4921 cgtacatcct tcggcaaggc ccgcatcgac gtgaccgacg cggccggatc ggcgaagtag
     4981 ttcttggtga gatagctcgt cagcgcccgc gccggttgat tgagcagcag ccccgagatc
     5041 aggatcggga gctgcagctc ggtctgggcc agcgcgggga tcaacgccca cagcgnnnnn
     5101 nnnnncccgc cgcagcgccc gccaggaacg agacccacag cacgaccgca ccgggaccgt
     5161 gcagttccag cgcgacgacc gaggcggtga acccgccgac caccatcgaa ccttccgcgc
     5221 cgatgtggat gatgccggtg cggaacggga tcgacgtggc caacgccatg ccgacgatgg
     5281 ggatcgcccg gttgatcagc gtgcgcggcc cgttgccggt gaaggcgccc gaccacatct
     5341 ccgaataggc gctgaacggg ttggcgcccg tgagcgccag gatcgcgccg gatacgacaa
     5401 aggccagcac caacgaggcc agcaccgggc tggccgccag tgcgaacaga cgctggcgag
     5461 cggcaggcat cagcgccgtg tcagccatgg gcgtgttcgt gctggagggc gccggtcatc
     5521 gccaggccga tctcgacatc ggtcgtcagg cgcgggtcga gctcggcgac gatgcggcct
     5581 tcgaacatca ccaggatgcg agtcgacagg gcaaggatct cccacaactc ggacgacacc
     5641 agcaggatgc cgcggtcggc gtcgcggtgc tcgatgatcc gcccgtggat gaactcgatc
     5701 gcgccgatgt cgacaccgcg ggtcggctgt tcgacgatca gcagcggcgc gtcgtgaccg
     5761 aactcgcggg ccaccaccac cttttgcagg ttgccgccgg agagctgccc gaccggttgg
     5821 tcgatgccgc tgaccttgat gtcgaactgc tccaccaggt cggccgcgtg acgacgcacc
     5881 ggccgccgct tcagccaacc gccacgctgg aaccggcgcc gccactggta gcccatcagc
     5941 aggttgtcgc tggtgctggc cgggccagcc gaaccgacgg catggcgatc ttcgggcacg
     6001 taggcgatgc cccggtcgcg gcgggcgcgc acgccgtgat cgtccaacac gtgaccggcg
     6061 agggtgatct ggccgccggc gaccgcccgc aacgcgatca acgcctcgac cagttcggac
     6121 tggccgctgc cggtgacccc ggcgatgccg acgacctcac ccgcagcaac gctgaagctc
     6181 accccggcga cgcggggcaa gccgtcgtcg tcggtcacgt cgaggtcgcg cacctcgagc
     6241 agcggcgctc ccggttcgtg ctcgtgggga cgcttgccgg tgtcgacgct gcgcccggtc
     6301 atcgccagcg acagcgctgc cggtgacgtg cccgctgtga gcgcttcgtc gaccacccgg
     6361 ccacggcgca gcacggtgac ccgatcgctc agcgacatca cctcgttgag cttgtgggtg
     6421 atgaacacga tggtgcgccc ggctgcgcgc aaggtgcgca acacgtcgaa cagactgtcg
     6481 cgctcttgcg nnnnnnnnnn nnnnnnnccg cggtcggctc gtcgaggatc agcacctgcg
     6541 cgtcgcgata cagcgccttc aagatctcga cccgttgcat ctcgcccacg ccgagttgct
     6601 cgacgcgggc ggtggggtcg actccgaggc cgaactcggc ggccaatgcc gccacccgct
     6661 cggtggccgc ggcccggtcg aggaaccagc ctttgcgcgg ctcgtggccg tagaccacgt
     6721 tctcggccac cgtcatggtg ggaaacagct ggaacgactg gtggaccatg cccagccccg
     6781 ccgcgatcgc gtcgagagcc gagcggaact ggaccggttc accccggacg acgatcgaac
     6841 cgtcgtccgg ggtgaccagc ccgaagagga tggacatcag tgtcgacttg ccggctccgt
     6901 tctcgccgac gatcgcgtgg atctcacccg catgcacgga gagatcgatg cggtcgttgg
     6961 cgaccacgtc gccgaatcgt ttcgagacct gctcgagctg gagctcgacg ctcatgtccc
     7021 cgcctgagcc tggatcagcc gccggtgacc gggtccggca cgacgatggt gccggcgacg
     7081 atcgcgtcac gggcggtctt gaccgccgcg atcacgtccg ggtggtcggc gatcaggcag
     7141 ccgctgctgg cgacgtcgtc ctgcaacgcc accacaccca cgccgccttc ggccaggccg
     7201 aacgacacga ccgaaccggc cgtgccggcc aacacctggt cgatcaactg ctcggtgagc
     7261 acgtcgacca gcttcagggc attgtcgacg atgtggccgg gggccagcgc gcactggttg
     7321 acgtcggtgc cgtaggccag gaagtcctcc tcctgagccg cttcgaacac accgccgttg
     7381 gaacccgacg ccgcggcgaa gatctggtcg accccctggg cgtgcagggc gagagcctgc
     7441 tccttggcgg tcgccgggtc ggcgaagggg ttgtcgccgg cgatgaacac ctgcgagtcg
     7501 gtgatgtcgg cgttgaccga ctgggcaccg agcgcgaagt tgtcggtgaa gcggtgcagg
     7561 aacgggatgt cgagcgcgac gaccgagccg accttgccgc tctcgctgag cgacccggcg
     7621 atcacgccga gcaggtacga catctcgttc tcacggaaca cgccgcagtg caggttggcc
     7681 gggggctcgg ccgggcactg gtcgatcagg atgaactgct gggtcgggtt tgcagcagcg
     7741 aactcggccg acaggtcggt gaagtcgaac gtggtgagga tgatgatgtc gggcttctcc
     7801 tcgacggccg cttccaggtt ggcgcgtcgg ctgttgagat cggtcgactc gaacgtcttg
     7861 gacgtgccgc cgagacgggc tgcggcggcc tcggtgccga tcttgcccag ttcgaggaac
     7921 ttgttcggcc cgatgttggt gggggtgatg tagatgaagc tcgggccgtc gcccgtcgag
     7981 gcggcggtgg tcgtgctgtc tccgcccgcc gcagcggtcg tcgtggtcgt ctgctctgac
     8041 ttcttctcgt cactgccgca ggcgctggcg agcacgccga gcaatgcgac tgctccgacg
     8101 gccgagcgca agcgccgggt gttgttgctg ttcatgggtc tccctcgggt attgggttca
     8161 cggtgcctgt gttggcggtg ccgctggtcg acgcgtgctc agatcgcctc nnnnnnnnnn
     8221 nnnnnnnnng tgcggaattg gacgacgcga cggtcggtga cgagcgcgct gatcagcgcc
     8281 accggggtca cgtcgaaagc agggttgatc gtacgagtcc ccggcgggct gacgggggca
     8341 ccgcgaaacg agcagacctc gtcgtcgcca cgatcttcga tctcgatctc gtcgccgctc
     8401 gcggtggcaa ggtcgacggt ggattcgggg gccacgacca cgaacgnnnn nnnnnnnnnn
     8461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc
     8581 gatcagccga tgcggagcgc ccatgcgggc caactcccat gtggtgagcc gaccgccttg
     8641 caggagcggg cgcgtctcca gcgggatcgc ctcggtcagc ttgccccgct cgtgcagggt
     8701 ctggatgacg gccagcgcgg tgccccgctc gaccgtggca agaccgccgg tattgcagat
     8761 ggtcatcact cgcaacgggc ggtcgccggc caactccaac agcaggtcgg cgccgagctc
     8821 acccatcgcg gtggacgcgg cgagctcttc gtcacggatc gcatcggctt cggccaacag
     8881 cacatcgaag cctttttcgg ccagggccgc ggtgcggtcg accatcttgg ccaagttgac
     8941 cgctgtcggg cgggcgttgc gcactgccgc gacggccgcc gcgaactcgt cgggctgcac
     9001 ggccggatcg tgcaggctgg ccgcgagcgc gacaccgtag gcaccggcca caccaatcgc
     9061 cggggccccg cgcaccacta ggcggacgat cgcgtcggac agcgcatcga tgctggcgca
     9121 gcggaccacg acctcctcgc tcggcaaccg ggtctggtcg atcagttcga tcgccccatc
     9181 gacccatgcc acggtcggca cccgatcagc ggtgtcgaca ccagcggggt cgagaccagc
     9241 ggggtcgaga ggggtgttgt cagacaacat cggggtcacg ctacagtagt tgaccagatt
     9301 ggtcaagatt taagggcacc gcgcctggac ctcaccgaac ccggatcagc gtgaactgga
     9361 agcgaccttc gatgaaccac gtccgcgaga acaccaccgc ccggccggtg gtgtcgaagt
     9421 gggtctgctc gagcagcagg aacagtgcgt ccgcgggatg ctcgccccag ccgatctcct
     9481 cgccacgcga cgcatgcagg ttggtcaacg ccgacgcggc gaccacgccg tgggcgtcca
     9541 tcactccgaa catcgacccg gcgaccgagc ggatgtcgaa atccgccccc agcaggtcca
     9601 ccggcagcac ctcgtgcgag tacgccacca cgtcgtcgtc ggccgtgatc tcgcgatggg
     9661 tggccagcac agaagtgccg ggctgcagca gcaaggcctc gatctcgcaa tcggtggctg
     9721 cgcggatctg gatcgagcgg aagttcgaac ccggcttacg gccgagccgg cggatcgtct
     9781 ccgacatcga ctccagccgc tgcaggttgg ccgacaccgc gctggtcacc gccgcggcaa
     9841 acgtgcccaa cccacgccgt gtcacgatca gcccccggct gtcgagtgcg cgcaacgccg
     9901 tgcgcaccgt cgctcgcgac acgccgtacg cggccgccaa ctcgtgctcg gtcggcagcc
     9961 gttccgacgg gcgccactcc ccggcttcga ggcgcacccg cagactgtcg gcgagtcgct
    10021 cggacaggtt ggtaccgcgc gcgccagcag cagcgagcgg ctcgtcggtc gtcatcggcg
    10081 ggcacaatag cggtgggagt gtcgccgcca gcacagctac gttgtctgac aaccaggggg
    10141 cgccatgatc acaatcgact tgagcgacac cgtggtcgcg gtcaccggtg cctcgggtgg
    10201 gatcggcgct gccatcgccg ctcgcttcac cgacgcgggc gcagccaccg tcgtgcatca
    10261 ccgtcgcgag ccggtaccgc aggtgggtcc gctgtctgtt gccgtctcga tcgacctggc
    10321 gaccgacgac ggtccgcagc ggttgatcga cgcggcagtc gccacgttcg ggcggctcga
    10381 cgccgtgatc aattgcgccg gcgtgcaacc cgtggtcgac tttgcctcga tgacgacgcc
    10441 cgagtgggac gagatgatcg cggtcaacct caccgcggtc caccgcgtca cacaggcctt
    10501 cgcccgtcac gctgccgaac gcggtggtgg cggtgccgtg gtccacgtgg cctcgatcga
    10561 agggcaccag cccgcgcccg gtcatagcca ttacgcggtc gccaaggcag gcgtgctgat
    10621 gcacgcaaag gcagcggcga tcgagctcgg gtcgatcggc gtgcgggtca acaccgtgtc
    10681 acccgggctg gttcatcgcg acggcatcga gcacgcctgg cccgacggtg tcgcccgctg
    10741 gcgagccgcc gcgccgctcg gtcgtctcgg cgcacccagc gagatcggcg atgcctgcgt
    10801 gttcctgtgc tcgccgctcg cagcctgggt caccggcacc gacctcgtcg tcgacggcgg
    10861 cgtctcggcg aggccgacat ggtgaacgag gcaacggtgc gcaccgtgcc gaccggcgac
    10921 ggcccggttg gcgacgagct cgaccagtgc gtccatgcct cacggctgct gggggccgat
    10981 ccgacgctgg tcctgcacgg cggcggcaac acctcggtca agatgccgtg gcgcgacatc
    11041 accggcgcgg tgatcgaggc cctctacgtc aaaggttccg ggtgggacct ggccatcatc
    11101 gagcgggccg gcttcgctcc cctgcccctc gcccgcctgc gcgccctgct cgcgttggag
    11161 acgctcagcg acccggacat gatgcgcgag ctgtcggcgg cgcggctcga cccggccgcc
    11221 ccgcagccgt cggtcgaagc gctgctgcac gcgttcttgc cacatcgcgt cgtgctgcac
    11281 agccacgccg acctcatcct caccctgacc aacacccccg acggtgacgc gatcgtgcgc
    11341 gaggtgttcg gcgacagcgt ggtggtcgtc ccctacgtga tgccgggctt cgacctggcc
    11401 cgcgaggttc tcgcccgctg ggatgccgac tcccaccccg gcacggtcgg catcgtgttg
    11461 atgaaccacg ggctgttcag cttcgccgac accgcggagg ctgcctatcg ctcgcatctc
    11521 gacctgatcg acctggctcg ccaacgcgtc gcggccagcc ctcccctggc gcatcgcgac
    11581 cctgcggtcg cggccaacgc acacgcctgc gcgtgccagt tggctgcgct gcgtcagacg
    11641 atctcggccg cggcggggca accgatgatc gtcacccgcg acacgtcggc cgacacgatg
    11701 accttcgtcg ggcgccacga cctggccgac gtcgccaccc gcggaccggt gacccccgat
    11761 cacgtcatcc gcaccaagcg ggtcccgctc gtcggtcgcg acgtcgacgc gttcgtggcc
    11821 gactatcgca gctattacga cgagcacgaa caccgctcgc gcacaccgat caccatgctc
    11881 gaccccgcac cgcgggtgct gctcgacccc gagttgggca tgctcacggt cgggcaccgt
    11941 cccgccgacg ccgccatcgc ggccgacatc taccgccaca ccatgcaggt catcaccacc
    12001 agcgaggagc gcctcggtgg gtgggcgccg ttgccgccgt tccacctgtt cgacctcgaa
    12061 tactgggact tggag
//
LOCUS       DAORNF010000008        12020 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_4013_length_12020_cov_1.901880, whole genome shotgun sequence.
ACCESSION   DAORNF010000008 DAORNF010000000
VERSION     DAORNF010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 12020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12020
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4013_length_12020_cov_1.901880"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            302..1420
                     /locus_tag="PLV68_00715"
     CDS             302..1420
                     /locus_tag="PLV68_00715"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HRD99784.1"
                     /translation="MRTSKKVLSALTISTVFGLTAVACGSDKKADTTTTTAAASSTTA
                     ASSTTAAPAAGKICEVTDVGGVDDKGFNESAWVALEESEKELGTSSKLLESSTEADYA
                     TNIQSLLGDNCALIVTVGFLLDGATASAANQQFAIIDSSALDNNGTPDDFSDDVALPN
                     VRPLNFATDQSSFLAGYLAAGMSETGVVGTFGGINIPPVSIFMEGFRLGVEHYNSVKG
                     TDVELLGWDGSEGSFAGNFDSLDDGKRIAQGFADEGADVIFPVAGPVGQGASAYALEK
                     GGIRIIGVDTDQYLSNPEHKAVYLTSVLKGITAAVKDTVANVVKDGKAGDNYVGTLEN
                     GGTSLSPFHDQDADVPQELRDELEALTADIIAGKIKTS"
     gene            1536..3053
                     /locus_tag="PLV68_00720"
     CDS             1536..3053
                     /locus_tag="PLV68_00720"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRD99785.1"
                     /translation="MELELRGITKRFPGVVANDDVNLTIRGGEVLALVGENGAGKSTL
                     MNILYGLYHADEGEILVDGTPVTFTSPADAIAAGIGMVHQHFMLVPVFTVTENVILGV
                     EPTMSLGRVDIASARKRVTELSSEFGLYLNPDAVTEDLAVGIQQRIEIVKVLFRDAQV
                     VVFDEPTAVLTPQEIEDFFRIVETLKAAGCAVVFITHKLKEALRIADRIAVLRRGKTV
                     GETTPAATTQEELAEMMVGRPVDLVVKKKPAEPGEVVLAVSDLTVADASGRAMVDHVG
                     FQVAAGEIVGIAGVQGNGQTELVETLVGLRRASSGTIMLSGHDVTHDTPREHHQAGVG
                     HIPEDRQRVGMVKDFSVTENLVLTGYYEPPLSNGLNMNWGEANRVAVQLVENFDIRTP
                     SVETPVGTLSGGNQQKVIVAREISRPLTLAIACQPTRGVDVGSIEYIHEQLVGLRDQG
                     IAVLVVSTELDEVMALSDRVLVMFDGRIVANLARAATDHNDVGLYMAGAKEDAAA"
     gene            3050..4249
                     /locus_tag="PLV68_00725"
     CDS             3050..4249
                     /locus_tag="PLV68_00725"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRD99786.1"
                     /translation="MTTATIPEHIAPEPAAASPWPQRLRQARLTALAILTALIIGALV
                     VAISDIDQLKRFEVGGMFRDAVDAYKALGSGAFGSLRGWSETITAATPLIFAGLAVAV
                     SFKAGLFNIGATGQMLAGGLAATYVGFNFSFPALIHVPLCLIAAIVAGGIWGGIVGLL
                     KARLGAHEVITTIMLNYVMFNLITWVLKTELFLRPGGAGNPISKQIKPSAELPYFFGF
                     TGRNLLLDLRAHSGFLVAIALAGLVYWLMTKSKLGFQLRTLGSNADAARYAGMSPPKL
                     TIAAMAMAGAFAGLGGASEIMGGVTGHRATSGFAGDIGFDAIAVALLGRSSPWGTTAA
                     ALLFGALKAGGDRMQATTGVPVDLVMIIRALIVLFVAAPNLTRTIWRLKDRGGESGSQ
                     LFRGWGS"
     gene            4246..>4473
                     /locus_tag="PLV68_00730"
     CDS             4246..>4473
                     /locus_tag="PLV68_00730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99787.1"
                     /translation="MTTPNAMPTPTEIYTDAQHGVLRSFEQIRRARNLGILSLVMAAV
                     TAWQFTGRAGSATFNLDSGRSFGIAATPFAWL"
     assembly_gap    4474..4536
                     /estimated_length=63
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4537..5497
                     /locus_tag="PLV68_00735"
     CDS             <4537..5497
                     /locus_tag="PLV68_00735"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRD99788.1"
                     /translation="FVLFIMSFLAWAAAGGRFAFVGMLAQMVKFATPLVFGALAGILC
                     EKSGVVNIAIEGMLLQGAFTSVLVGSLTAFWVGVVAAMVVGALFAWLLAWLAIRFKVD
                     QVIVGFFLNFLILGLTSFLDGRVLSKNPHYNNVTTVRPWNVPLLERIPVIGAIFFRQT
                     IYVYIAYIAVFFLAWAFAKTRWGLRTRAVGEHPKAADTLGVNVNRTRYRNVIMAGALA
                     GFGGSWYIADIGKFRENMVGGRGFIALAVVIIGRWSPIGALAGATTFGFFIALQDKLG
                     ILKTGLPSEFVAMLPYIATIVVVAGVGGKSRAPKAAGQPYESQ"
     gene            complement(5544..5972)
                     /gene="queF"
                     /locus_tag="PLV68_00740"
     CDS             complement(5544..5972)
                     /gene="queF"
                     /locus_tag="PLV68_00740"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HRD99789.1"
                     /translation="MTVDESRSAGTDVAVDTSGLTILGNTVRHPVPHVEVFPAPANVT
                     VVRFTNDELNSMCPVTQQPDLSTVVIEYVPAQHCVESKSLKLYLWSFRDRAVFAEAMA
                     AEIAGEIMASAHPQRVTVTITQRPRGGIELQATSELPSRA"
     assembly_gap    6124..6133
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6134..7011
                     /locus_tag="PLV68_00745"
     CDS             <6134..7011
                     /locus_tag="PLV68_00745"
                     /inference="COORDINATES: protein motif:HMM:NF017532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HRD99790.1"
                     /translation="SGVVYLTPTHPDSRTVFTTNEQERVSNDEFLDSANGSWCFFEED
                     AFAPDGSLVQDASLSINKIGHAMHDLDDVFRRFSYTPELAGVAADVGLTDAIALQSMY
                     IFKQPRIGGEVGCHQDATFLYTEPMTVTGFWFAIEDATLTNGCLWAAPGGHRTTLRTV
                     FKRNSTANDGGTHTEVLDTTPLPSPAELVPLEVTHGTLVVLHGLLPHWSDVNRSEHSR
                     HAYSLHCVSGAADYPWWNWLQRSPDLPLRQLSTGAPGPAAEGAPATRIQMGRGAQPSG
                     GAAPETNDLLVGSAT"
     gene            7008..8084
                     /locus_tag="PLV68_00750"
     CDS             7008..8084
                     /locus_tag="PLV68_00750"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HRD99791.1"
                     /translation="MIEQLLQRAPKALLHDHLDGGLRPETVIELADQFGYTNLPTHDV
                     AELASWFNRGAKRNDLVLYLETFAHTCGVMQHAAALERVAYECAQDLAADNVVYAEVR
                     FAPELHIEAGLTLDEVVEAVMAGFARGAAGTDLTIYALLCAMRTAARSLDIAELAVRW
                     RDRGVVGFDIAGAEAGYPPTRHLDAFQYVQRENFHSTIHAGEAFGLPSIWEAVQFCGG
                     ERLGHGVRIVDDITGAPGDEKLGRLAAYIRDRRIPLELCPTSNVGTGVCASVAEHPIG
                     MLRRLRFRVTINTDNRLMSATSMTHEMLELHRAFGWGLDDMEWLTTNAMKSAFAPFPE
                     RLRIINGIVKPRYALLRAEQSLGV"
     gene            8155..8787
                     /gene="upp"
                     /locus_tag="PLV68_00755"
     CDS             8155..8787
                     /gene="upp"
                     /locus_tag="PLV68_00755"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HRD99792.1"
                     /translation="MSVEVHLVEHPLAAASLTKVRDENTPNAIFRQELERIGTLVLVE
                     ATRNLATHTVEVKTPVTMATGAILTTQPVVIPVLRAGLGFLHAAQVLLPGADVGFVGI
                     SRDEVTFEPKPYVNKLPESFEDRVCIVLDPMLATGGSLGHTCKLLKDHGARGPIITVC
                     ALAAPEGILHLHDYDVDIVVYTASIDERLNENKFIVPGLGDAGDRQFGAA"
     gene            complement(8819..>9003)
                     /locus_tag="PLV68_00760"
     CDS             complement(8819..>9003)
                     /locus_tag="PLV68_00760"
                     /inference="COORDINATES: protein motif:HMM:NF012626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99793.1"
                     /translation="IAGEAVTSAEWFAEAGLLRLWLGDIRLQVRPSGTEPKVKLYGEA
                     IGQDPGPYLDALAELL"
     assembly_gap    9004..9062
                     /estimated_length=59
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9063..9491)
                     /locus_tag="PLV68_00765"
     CDS             complement(<9063..9491)
                     /locus_tag="PLV68_00765"
                     /inference="COORDINATES: protein motif:HMM:NF014879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99794.1"
                     /translation="MVLADGGWRRLGGDEIGWLLADHILSHTTGDDRLVITTLVSSSL
                     LGRLAASYGVHFLETYTGFKWIGRTAMDHPEWRFLFGYEQALGYLVAPQPMDKDGITA
                     AVMLAEVAALAAAEGVTLHDRLDDLAARFGQHVVAERSVRL"
     assembly_gap    9515..9606
                     /estimated_length=92
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9607..>9948)
                     /locus_tag="PLV68_00770"
     CDS             complement(<9607..>9948)
                     /locus_tag="PLV68_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99795.1"
                     /translation="KVYLGDGAQIVPPHDAGIAACIDEFDPTGVDATGIELAEPDDPR
                     IVRLDDQPLTRYLASVPAVRLRPDLPGVAVAYTAMHGVGGATALRAFELSGLPAPAVV
                     EAQQQPDPTFPT"
     assembly_gap    9949..9996
                     /estimated_length=48
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9997..10426)
                     /locus_tag="PLV68_00775"
     CDS             complement(<9997..10426)
                     /locus_tag="PLV68_00775"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="catalyzes the interconversion of alpha-D-glucose
                     1-phosphate to alpha-D-glucose 6-phosphate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HRD99796.1"
                     /translation="MAETDIRDRAQRWLVAEPDDDVRAEMAALLEEPAEVLAARFDGR
                     LQFGTAGLRGPIGAGPLRMNRLVVRQAAAGLGLYLLRTVEDAAERGVIIGYDARRKSD
                     VFALDTARVLAAMGVKALLLPAPLPTPVLAWNIVPHNAAAG"
     gene            complement(10484..>11422)
                     /locus_tag="PLV68_00780"
     CDS             complement(10484..>11422)
                     /locus_tag="PLV68_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine kinase"
                     /protein_id="HRD99797.1"
                     /translation="EDHEAFRSSVKEFLDREVLVKGSMTLIDTERAVSLYSALGNAIE
                     MSGGSAANTMVGIASFGGRAAYVGKVNDDELGEVFGHDLRAVGVAFRSAQPDSAVPTG
                     RCIIVVTPDAQRTMNTYLGVSSLFSPPDIDADVVAAGKVLYMEGYLYDRHPAKEAFRV
                     AASIAHANGRQVSLTLSDSFCVDRHRSDFRGLVADEIDILFGNEAELMSLYEVDDFDA
                     AVAAVGRDCSLAAITRGARGCVVVTPDGLIPVAAEPVDRVLDTTGAGDLFASGFLYGY
                     TQGIDLAECARLGSLAASEVISHVGARPLIELKTLL"
     assembly_gap    11423..11452
                     /estimated_length=30
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            11568..>12020
                     /locus_tag="PLV68_00785"
     CDS             11568..>12020
                     /locus_tag="PLV68_00785"
                     /inference="COORDINATES: protein motif:HMM:NF037667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99798.1"
                     /translation="MSLDPYRLPRTVVPRHYAVTLEPELAEARFTGTVSISVEVTEAV
                     GQIVLNAIELDVSAVRVDGHDAPFHLDVVRERLFITFAHGAGPATIDISFTGILNDKL
                     RGFYRSTYRDDAGTEQVIATTQMQATDCRRAFPCFDEPALKAVFSVTLV"
BASE COUNT         1970 a         4131 c         3780 g         1837 t
ORIGIN      
        1 ccgttgtcct gggcggtacg cccacccgcc gatggcacgg ccgccggacg cggcggcaca
       61 gggcgatggc ggctgtcatc gccgctggcg ggactggagg gagggggcgg gcggcgctcg
      121 cgtcggtcgt ccatgcgtct cgcccatcgt aggcaacacg gcccgttcac aggtgacgca
      181 cgacgcatgg atggttggga tgttccagcc gcggtacgct gcggcaatcg gattcctccg
      241 atgtgaacaa ccgaaccagc aattcccagg aattcaacga cactcaaaac aagggggagc
      301 aatgcggacg agcaaaaagg tgctcagcgc gctcacaatc agcacggtct tcggcctcac
      361 cgccgttgcc tgtggcagcg acaagaaggc cgacaccacg acgaccaccg ccgccgccag
      421 cagcacgact gcggcgagca gcaccacggc ggctccggcc gcaggcaaga tctgcgaagt
      481 gaccgacgtc ggcggtgtcg acgacaaggg cttcaacgag agcgcatggg tcgccctgga
      541 agagtccgaa aaggagctcg gcacctcgag caagctgctc gagtcgtcca ccgaggccga
      601 ctacgccacc aacatccaga gcctgctcgg cgacaactgc gccctgatcg tcacggtcgg
      661 cttcctgctc gacggcgcga ccgcatcggc ggccaaccag cagttcgcga tcatcgactc
      721 cagcgcgttg gacaacaacg gcacgccgga cgacttctcc gacgacgtcg ccctgcccaa
      781 cgtccgcccg ctcaacttcg ccaccgatca gtcgtcgttc ctcgccgggt acctggctgc
      841 gggcatgagc gagaccggtg tcgtcggcac cttcggcggc atcaacatcc cgccggtgtc
      901 gatcttcatg gagggcttcc gcctcggcgt cgagcactac aacagcgtca agggcaccga
      961 cgtcgagctg ctcggttggg acggcagcga aggctccttc gccggcaact tcgacagcct
     1021 cgacgacggc aagcgcatcg cccagggctt cgccgacgaa ggtgccgacg tgatcttccc
     1081 ggtcgccggc ccggtcggcc agggtgcgtc ggcctacgcc ctcgaaaagg gtggcatccg
     1141 catcatcggc gtcgacaccg accagtacct cagcaacccc gagcacaagg ccgtctacct
     1201 cacctcggtg ctcaagggca tcacagcagc ggtgaaggac accgtcgcca acgtggtcaa
     1261 ggacggcaag gcgggcgaca actacgtcgg caccctcgag aacggtggca ccagcctgtc
     1321 cccgttccac gatcaggacg ccgatgtgcc ccaggagctc cgtgacgagc tcgaggcgct
     1381 gaccgccgac atcatcgctg gcaagatcaa gaccagctga ttcacacgcc cctccgtgcg
     1441 gaggcggcgc atcgaacgag cgagagggac ccattccggg tccctctcgt cgttttccgt
     1501 tcgactaggg ttcgcccaac tcgaaagggg caaggttgga actcgagcta cgcggcatca
     1561 cgaaacggtt ccccggcgtc gtcgccaacg acgacgtcaa cctcaccatc cgcggcggtg
     1621 aggtactggc cttggtgggc gagaacggtg ccggcaagag cacgttgatg aacatcctct
     1681 acggcctgta tcacgccgac gagggcgaga tcctcgtcga cggcacgccg gtcacgttca
     1741 cctccccagc agacgcgatc gcggccggga tcggcatggt ccaccagcac ttcatgctgg
     1801 tgccggtgtt caccgtgacc gagaacgtca tcctcggcgt cgagccgacg atgtcgctcg
     1861 gccgggtcga catcgcctcg gcccgcaagc gggtcaccga gctgtcgtcc gagttcggcc
     1921 tgtatctcaa ccccgatgcg gtgaccgaag acctcgccgt cggcatccag cagcggatcg
     1981 agatcgtcaa agtgctgttc cgcgacgcgc aggtggtcgt gttcgacgaa ccgaccgcgg
     2041 tgctgacccc gcaggagatc gaagacttct tccgcatcgt cgagacgttg aaggcggccg
     2101 ggtgcgcggt ggtgttcatc acccacaaac tcaaagaggc actgcgcatc gccgaccgca
     2161 tcgccgtgct gcggcggggc aagaccgtcg gcgagaccac accggctgcg accacgcaag
     2221 aagaactggc cgagatgatg gtcggccgcc cggtcgatct ggtggtcaag aagaagccgg
     2281 ccgagcctgg cgaggtcgtg ctggcggtca gcgacctgac cgtggccgat gccagcggcc
     2341 gggcaatggt cgaccacgtc gggttccagg ttgctgctgg cgagatcgtc ggtatcgccg
     2401 gcgtgcaggg caacgggcaa accgaactgg tcgagacgct ggtcggcctg cgccgggcgt
     2461 ccagcggcac gatcatgctc agcggtcacg acgtcaccca cgacacaccc cgtgaacacc
     2521 accaggctgg tgtgggccac attcccgaag accgccagcg cgtcggcatg gtcaaggact
     2581 tcagcgtcac cgagaacctg gtgctcaccg ggtactacga accgccgctc agcaacgggc
     2641 tgaacatgaa ctggggcgag gccaaccgcg tcgccgtcca gttggtcgag aacttcgaca
     2701 tccgcacccc ttcggtcgag acgcccgtcg gcaccctgtc gggcggcaat cagcagaagg
     2761 tgatcgtcgc ccgcgagatc agccgcccgc tcaccctggc catcgcctgc cagcccaccc
     2821 gcggcgtcga cgtcggctcg atcgagtaca tccacgaaca gctcgtgggc ttgcgcgacc
     2881 aaggcatcgc tgtgctggtc gtcagcacgg agttggacga ggtgatggcg ctgtcggatc
     2941 gggtgttggt gatgttcgac gggcggatcg tggccaacct ggcccgcgcc gccaccgacc
     3001 acaacgacgt cggcctgtac atggccggcg ccaaggagga cgcggcagca tgaccaccgc
     3061 gaccatcccc gaacacatcg cccccgaacc ggccgcggcg tcaccctggc cgcaacggct
     3121 gcgccaagca cgcctcaccg ccctggccat cctcacggcg ctgatcatcg gcgcgctcgt
     3181 cgtcgccatc tccgacatcg accaactcaa gcggttcgag gtgggcggca tgttccgcga
     3241 cgccgtcgac gcctacaagg cgctcggcag cggcgcgttc ggcagtctgc gcggctggag
     3301 cgagacgatc accgccgcga ccccgctgat cttcgccggc ctcgccgtgg cggtgtcgtt
     3361 caaggccgga ctgttcaaca tcggcgccac cggccagatg ctggccggcg ggctggcagc
     3421 gacctacgtc gggttcaact tcagctttcc ggcgctgatc cacgtgccgc tgtgcctgat
     3481 cgccgcgatc gtcgccggcg gcatctgggg tggcatcgtc ggcctgctga aggcgcgtct
     3541 cggcgcccac gaggtgatca ccacgatcat gctcaactac gtgatgttca acctgatcac
     3601 ctgggtgctc aagaccgagc tgttcctgcg ccccggcggt gccggcaacc cgatctccaa
     3661 gcagatcaag ccatcggccg agctgccgta cttcttcggc ttcaccggcc gcaacctcct
     3721 gctcgacctg cgcgcccaca gcgggttcct ggtcgcgatc gcgctcgcgg ggctcgtcta
     3781 ctggctgatg acgaagtcga agctgggctt ccaactgcgc accctcggct ccaacgccga
     3841 cgcggcccgc tacgcgggca tgagcccgcc caagctgacg atcgcggcca tggcgatggc
     3901 cggcgcgttc gccggcctcg gcggggcgag tgagatcatg ggtggtgtca ccggccaccg
     3961 cgccacatcc ggcttcgccg gcgacatcgg cttcgacgcc atcgcggttg ccttgctcgg
     4021 ccgctccagc ccgtggggca ccacggccgc agcactgctg ttcggcgcgc tcaaggcggg
     4081 cggcgaccgg atgcaggcca ccactggtgt accggtcgac ctggtgatga tcatccgcgc
     4141 cctgatcgtg ctgttcgtcg ccgcaccgaa cctgacccgc acgatctggc gcctgaagga
     4201 caggggcggc gaatccggca gccaactgtt ccgggggtgg ggatcgtgac gacaccaaac
     4261 gccatgccga caccgaccga gatctacacc gacgcccaac acggcgtgct gcgctcgttc
     4321 gagcaaatcc gccgggcccg caacctgggc atcctctccc tcgtcatggc ggccgtgacc
     4381 gcgtggcagt tcaccggacg ggccgggtcg gccacgttca acctcgactc cgggcgcagc
     4441 ttcggcatcg ccgcgacccc gttcgcctgg ctgnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngttc gtgctgttca tcatgtcgtt
     4561 cctggcatgg gcggcagccg gcggccggtt cgcgttcgtg ggcatgttgg cccagatggt
     4621 caagttcgcg accccgctcg tgttcggcgc gttggcaggc atcttgtgcg agaagtcggg
     4681 cgtggtcaac atcgccatcg agggcatgct gctgcagggt gcgttcacct cggtcctcgt
     4741 cgggtcgctg accgccttct gggtcggcgt cgtcgcggcg atggtcgtgg gcgcgctgtt
     4801 cgcgtggctg ttggcctggc tggccattcg cttcaaggtc gaccaggtga tcgtcgggtt
     4861 cttcttgaac ttcctcatcc tcggcctcac gtcattcctc gacggccgcg tgctcagcaa
     4921 gaacccgcac tacaacaacg tcaccaccgt gcggccgtgg aacgtcccgc tgctggaacg
     4981 catcccggtg atcggggcga tcttcttccg ccagacgatc tacgtgtaca tcgcctacat
     5041 cgccgtgttc ttcctggcat gggcgtttgc caagacccgc tgggggctgc gcacccgcgc
     5101 cgtgggcgaa cacccgaagg cggccgacac cttgggcgtc aacgtcaacc gcacccgtta
     5161 ccgcaacgtg atcatggctg gtgcgctggc cggattcggc ggatcgtggt acatcgccga
     5221 catcggcaag ttccgcgaga acatggtcgg cgggcgtggg ttcatcgctt tggccgtcgt
     5281 gatcatcgga cgctggagcc cgatcggtgc cctcgccggg gcgaccacgt tcggcttctt
     5341 catcgccttg caagacaagt tgggcatcct caagaccggc ctgcccagcg agttcgtggc
     5401 gatgctgccc tacatcgcca ccatcgtcgt ggtcgccggt gtcggcggca agtcccgcgc
     5461 ccccaaagcc gccggccaac cctacgagag ccagtagccc cgccgccgcg acgagcggcc
     5521 gcagtccggg catccggact gacttacgcg cgcgacggga gttcgctggt ggcttggagt
     5581 tcgatgccgc cgcgggggcg ttgggtgatg gtgaccgtga cccgctgcgg gtgggcactg
     5641 gccatgatct caccggcgat ctcggccgcc atcgcctcgg cgaacacggc ccggtcgcgg
     5701 aagctccaca ggtacagctt gaggctcttg ctctcgacgc agtgctgcgc gggcacgtat
     5761 tcgatgacca ccgtcgacag gtcgggctgc tgggtgacgg ggcacatgct gttcaactcg
     5821 tcgttggtga aacgcacgac ggtgacgttg gcgggcgccg gaaagacctc gacgtgcggg
     5881 accggatgac gcaccgtgtt gcccaggatg gtgaggcccg acgtgtcgac ggcgacgtcg
     5941 gtacccgctg atctgctctc gtcgacggtc atggcacaca ggctacggtg agcccatgtc
     6001 aaccgtcctc accgaccggc aggtggccga ctaccaacgc gacggcttcc tcgtcgtccc
     6061 cgacttcgct gatccttccg cttgcaacga cctgcgcacg cgggccaacg agatcgtggc
     6121 cgcnnnnnnn nnntctccgg cgtggtctat ctgacgccga cgcatcccga ctcgcgcacc
     6181 gtgttcacca ccaacgaaca agagcgggtt tccaacgacg agttcctcga ctcggccaac
     6241 gggtcgtggt gcttcttcga agaggacgcg ttcgcgcccg acggatcgct ggtgcaggac
     6301 gcgtcgctgt cgatcaacaa gatcggccat gcgatgcacg acctggacga tgtgttccgc
     6361 cgcttcagct acacgcccga actcgccgga gtcgccgccg acgtcggtct caccgacgcg
     6421 atcgcgctgc agagcatgta catcttcaag cagccacgaa tcggcggcga ggtcggctgc
     6481 caccaggacg cgacattcct gtacaccgag ccgatgaccg tcaccgggtt ctggttcgcg
     6541 atcgaagacg ccaccctcac caacggctgc ctgtgggcgg cacccggtgg ccatcgcacc
     6601 acgttgcgca cggtgttcaa gcgcaacagc accgccaacg acggcggcac tcacaccgag
     6661 gtgctcgaca ccacgccgct gccctcaccg gccgagttgg tgccgctgga agtcacgcac
     6721 ggcacgctgg tggtgctgca cgggctgctc ccccactgga gcgacgtcaa ccgcagcgag
     6781 cacagccgtc acgcctactc gctgcactgc gtgtcgggcg cggccgacta cccgtggtgg
     6841 aactggctgc aacgctcccc cgatcttccc ctccgccagt tgagtaccgg cgcgccaggc
     6901 ccagctgccg agggcgcgcc agcgacccgg atccagatgg ggcgcggcgc gcagcccagc
     6961 gggggcgcag cccccgagac gaacgacctc ctggttgggt cggcgacgtg atcgagcagt
     7021 tgttgcagcg agctccgaag gcgctgctcc acgatcacct cgacggtggg ttgcgccccg
     7081 agacggtgat cgaactggcc gaccaattcg gctacaccaa cctgcccacc cacgacgtgg
     7141 ccgagctggc gagctggttc aaccgcggcg ccaagcgcaa cgacctggtg ctgtacctgg
     7201 agaccttcgc ccacacctgt ggggtgatgc agcacgctgc agcactcgaa cgggtcgcct
     7261 acgagtgcgc ccaagacctg gccgccgaca acgtcgtcta cgccgaggtg cgcttcgccc
     7321 cggaactgca cattgaggcg ggcctcaccc tggacgaggt ggtcgaagcg gtgatggcag
     7381 ggttcgcccg cggcgcggcc ggcaccgacc tgacgatcta cgccttgctg tgtgcgatgc
     7441 gcaccgcggc ccgctcgctc gacatcgccg agttggccgt gcgctggcgc gaccgcggcg
     7501 tggtcggctt cgatatcgcc ggggccgagg cgggataccc gcccacgcgc cacctcgacg
     7561 cgttccagta cgtgcagcgc gagaacttcc acagcacgat ccatgccggc gaggccttcg
     7621 gcctgccctc gatctgggaa gcggtgcagt tctgcggcgg cgagcgcctg ggccacggcg
     7681 tgcgcatcgt cgacgacatc accggcgccc ccggcgacga gaagctgggc cgacttgcgg
     7741 cctacatccg cgaccggcgc atcccgctgg agctgtgccc cacctcgaac gtcggcaccg
     7801 gcgtgtgcgc ctcggtcgcg gagcatccca tcggcatgct ccgccgcctg cggttccggg
     7861 tcacgatcaa caccgacaac cgcttgatga gcgccacgtc gatgacccac gagatgctcg
     7921 aactgcaccg cgccttcgga tgggggctcg acgacatgga gtggttgacc accaatgcga
     7981 tgaagagcgc gttcgcgccg ttccccgaac ggctgcggat catcaacggc atcgtcaagc
     8041 cgcgctacgc gctgttgcgg gccgagcagt cgttgggcgt ctgacgtcac gatcggctcg
     8101 ccatctcatc tctgcctggc ggcgacgagc gcgccgcgtg tcaggatgag acccatgtcc
     8161 gtcgaagtcc acctggtcga acaccctctc gccgccgcat cgttgaccaa ggtccgcgac
     8221 gagaacaccc ccaacgccat ctttcgccag gagctcgaac ggatcggcac gctggtgctg
     8281 gtcgaggcca cccgcaacct ggcgacccac acggtcgagg tcaagacccc ggtcacgatg
     8341 gcgacgggtg cgatcctcac gacccagccg gtggtcatcc ccgtgctgcg cgccgggctc
     8401 ggctttctcc acgccgctca ggtgttgctg ccgggtgccg acgtgggctt cgtcggcatc
     8461 tcccgcgacg aggtcacctt cgaaccgaag ccgtacgtca acaagctgcc cgagagcttc
     8521 gaagaccggg tctgcatcgt gctcgacccc atgctggcca ccggtggatc gctgggccac
     8581 acctgcaagc tgctgaagga ccacggcgcc cgcggcccga tcatcaccgt ctgtgcgctg
     8641 gccgcccccg aaggcatcct gcacctgcac gactacgacg tcgacatcgt cgtctacacc
     8701 gcgtcgatcg acgagcgcct caacgagaac aagttcatcg tgcccggtct gggcgacgcc
     8761 ggcgaccgcc agttcggcgc cgcctgagcc aacaccgacg tcggcgctgc gaagcgggtc
     8821 agagcagttc ggcgagcgcg tcgaggtacg ggccggggtc ctggccgatc gcttcgccgt
     8881 acagcttgac cttcggctcg gtgccgctgg gtcgcacctg caggcggatg tcgcccaacc
     8941 acaaccgcaa caggcctgcc tcggcgaacc actcggcgct ggtgaccgct tcgccggcga
     9001 tctnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9061 nngagccgca ccgagcgctc ggcgaccaca tgctggccga agcgggcagc cagatcgtcg
     9121 aggcgatcgt gcagcgtgac cccctcggcc gctgccagcg cggcgacctc ggccagcatc
     9181 accgccgcgg tgatgccgtc cttgtccatc ggttgcgggg cgaccaggta accgagggcc
     9241 tgctcgtagc cgaacaggaa gcgccactcg gggtgatcca tcgccgtgcg gccgatccac
     9301 ttgaagccgg tgtaggtctc caggaagtgc acgccgtagc tggctgccag gcggccgagc
     9361 agtgacgaac tgaccaaggt ggtgatcacc agccggtcgt cgccggtggt gtggctgagg
     9421 atgtggtcgg ccagcagcca gccgatctcg tcgccgccca accgtcgcca cccgccgtcc
     9481 gccaaaacca atcgccaccg tatagccgct gacgnnnnnn nnnnnnnnnn nnnnnnnnnn
     9541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9601 nnnnnngtgg ggaaggtcgg gtcgggctgc tgctgggctt cgaccacagc gggggccggc
     9661 aggccggaca gctcgaacgc gcgcagcgcc gtcgcgccgc cgacgccgtg catcgcggtg
     9721 taggccaccg ccacaccggg cagatcgggc cgcaaccgca ccgcgggcac cgaggccagg
     9781 tagcgcgtga ggggctgatc gtcgaggcgc acgatgcgcg ggtcgtcggg ctcggccaac
     9841 tcgataccag tcgcatccac accagtgggg tcgaactcgt cgatgcaggc ggcgatgcca
     9901 gcatcgtggg ggggcacgat ctgggcgcca tcaccgaggt acaccttgnn nnnnnnnnnn
     9961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncccc cgccgcagcg ttgtggggca
    10021 cgatgttcca cgccagcacc ggcgtgggca gcggggcggg caacagcagc gccttgacgc
    10081 ccatcgccgc caacacccgc gccgtgtcca gcgcgaacac gtcgctcttg cgccgcgcgt
    10141 cgtagccgat gatcacgcca cgttcggccg catcctcgac cgtgcgcaac aggtacagcc
    10201 cgagcccggc cgcggcttgg cgcaccacca agcggttcat gcgcaacggt cccgcgccga
    10261 tcggtccgcg caggcccgcg gtgccgaact gcagccgccc gtcgaagcgg gccgccaaca
    10321 cctcggccgg ctcttccaac agcgcggcca tctcggcacg aacgtcgtca tccggctcgg
    10381 ccaccaacca ccgctgcgca cggtcacgaa tgtcggtctc agccatcgca acccccgttg
    10441 cactcgatga tgcggtccat gatgccgtgt cgtcagcgac gcgctacagc agcgtcttca
    10501 gttcgatcag cggcctggcg ccgacgtggc tgatgacctc ggaagcagcc agcgatccga
    10561 gccgggcgca ctccgccaga tcgatgccct gggtatagcc gtagaggaac ccggaggcga
    10621 acaggtcgcc agcgccggtg gtgtcgagca cccgatcgac cggttcggca gcaactggga
    10681 tcaagccgtc gggggtgacc acgacgcaac cgcgcgcgcc acgcgtgatg gccgccaacg
    10741 aacagtcgcg gccgaccgcg gcgaccgcgg cgtcgaagtc gtcgacctcg tacagcgaca
    10801 tcagttcggc ctcgttgccg aacaggatgt cgatctcgtc ggccacgagc ccacggaagt
    10861 cgctgcggtg acgatcgacg cagaacgagt cggacaaggt gaggctgacc tggcgcccgt
    10921 tggcgtgggc gatcgaggcc gctacacgga acgcttcctt ggccggatgc cggtcgtaca
    10981 ggtagccctc catgtacaac accttgccgg ccgcgacgac atcggcgtcg atgtcgggcg
    11041 ggctgaacaa cgacgacacc ccgagatagg tgttcatcgt gcgctgcgca tcgggcgtga
    11101 ccacgatgat gcaccgcccg gtcggtaccg cgctgtcggg ctgcgccgag cgaaaggcca
    11161 cacccacagc acgcaggtcg tggccgaaca cctcgccgag ctcgtcgtcg ttgaccttgc
    11221 cgacgtatgc ggcacggcca ccgaaggatg cgatgccgac catggtgttg gctgccgagc
    11281 cgccactcat ctcgatcgcg ttgccgagcg cgctgtacag cgacacggcc cgctcggtgt
    11341 cgatcagcgt catcgagccc ttgaccagca cctcgcggtc gaggaactcc ttgaccgagc
    11401 tccggaaggc ctcgtggtcc tcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncacagcgg
    11461 gctcggacac gctgggcaac gctatcgcca ccggcacgcc cagccaggac tcagcgcccg
    11521 gatcgattgg aacaacagtt tcataccgac aggtaatctg gtcggcgatg tccctggatc
    11581 cataccgtct tccccgcacc gtcgtgcccc gccactacgc ggtcaccctc gagcccgaac
    11641 tggccgaggc acgcttcacc ggcacggtct ccatctccgt cgaggtcacc gaggcagtcg
    11701 gtcagatcgt actcaacgcg atcgagctcg acgtcagcgc ggtgcgcgtc gatggccacg
    11761 atgcgccctt tcacctcgac gtggtcagag agcgactgtt catcacgttt gcccatggcg
    11821 ccgggccggc cacgatcgac atctccttca cgggcatcct gaacgacaag ctgcgcggct
    11881 tctaccgctc cacgtaccgc gacgacgccg ggaccgaaca ggtcatcgcc accacgcaga
    11941 tgcaggccac cgattgccgc cgggccttcc cctgcttcga cgagccggcg ctcaaggcgg
    12001 tgttctcggt cacgctcgtc
//
LOCUS       DAORNF010000009        11935 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_4050_length_11935_cov_1.733249, whole genome shotgun sequence.
ACCESSION   DAORNF010000009 DAORNF010000000
VERSION     DAORNF010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 11935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11935
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4050_length_11935_cov_1.733249"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="PLV68_00790"
     CDS             <1..297
                     /locus_tag="PLV68_00790"
                     /inference="COORDINATES: protein motif:HMM:NF012880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRD99799.1"
                     /translation="AGVAIERARRGDGPSFVEAVSVRNRGHFTGDLEPYRPLDRQLAD
                     PIQIAAARLVATGAAQAELDRINADAAAEMEAALADAKAAPLPGRDRLLAGVWA"
     gene            294..1304
                     /locus_tag="PLV68_00795"
     CDS             294..1304
                     /locus_tag="PLV68_00795"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HRD99800.1"
                     /translation="MNAGTPSTAEMVEMSYARAINAALAEEMERDERVFLMGQDVGKL
                     GGVFGVTKGLQQRFGPQRVIDTAIVEHAIVGGAAGAAMAGMRPVAEVQFADFLLLAGD
                     ETASKLGKWRFMHGGRLTVPVVVRAPMGAQGGVGAEHSQCLEGVFWHTGGLRIVVPST
                     PADAKGLLKTAIRSDDPVLFFEHRRLYRARGPVPVDPDVVVPFGQAAVRRSGRDVTLV
                     TWSALVEDCVRAAEQLAERGVSAEVLDLRTLQPLDRTSLAESIGRTGRVIVVHEAAKT
                     AGPGAEIAAIVAEEFFPLLRAPVRRVCGYDAPVPQSGVLEAMWLPSVDEIATAAEELV
                     AW"
     gene            1298..2455
                     /locus_tag="PLV68_00800"
     CDS             1298..2455
                     /locus_tag="PLV68_00800"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HRD99801.1"
                     /translation="MVTRSVRLPLMGETVTEAVIVRWLRRVGEPVVEGEALFEVSTDK
                     VDTEVPAPLSGTLTGILVGEGMTATIGQVIANVETTDTGDTLDGPDVDDGSVAAGGLV
                     AERAGLVEEPPAANGAPRVTPLPPRRSQPELRPSPERRPSASSSASAAVTPPAVSDEP
                     AWVPFSPMRRVIAARMTESLHVAAHTLVVMEADYENVARARAERPGLTYLPFVCRAIV
                     DALRDFPLVNAHVSDDGLRPFDVVNLGIAVDLDLDGLVVPVVQQAERLRLSGIAAAIT
                     EVAGRARARSLPSQAYDGGTITVTNAGGRGSLFGRPIINLPQAAIVETDAVEERLVGT
                     RDQSGALVVQLRRRGYLSMSYDHRAFDGAYACGFLSAVCRTISERDWAAEL"
     gene            complement(2481..2921)
                     /locus_tag="PLV68_00805"
     CDS             complement(2481..2921)
                     /locus_tag="PLV68_00805"
                     /inference="COORDINATES: protein motif:HMM:NF025292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HRD99802.1"
                     /translation="MDSHALLGTWTLVSFVRRIDGEPRHHMLGEHPSGLISYQADGHM
                     SAILTDPDRPWPAGVNFRDGSPADQAAAAALFSAYGGSYTLDGDVVTHHVRISAWPEH
                     VGTDLVRRVSWIDGDLLLTTEPVLTPSGKALDDQLRWHKLGYRE"
     gene            3106..>4260
                     /locus_tag="PLV68_00810"
     CDS             3106..>4260
                     /locus_tag="PLV68_00810"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRD99803.1"
                     /translation="MSFDLIIRGGEVVDGSGLPRYRADVGVTDGRITAIGRIRERAAR
                     EIDAEGHVVTPGFIDGHTHMDAQVMWDPLGTCSSLHGVTTAVMGNCGFTLAPCRDGAD
                     GRELVVANIERAEDISAEAMAAGIDWTWSNFDGYLDAVGSRQFAINYAANIGHSALRT
                     WAMGAAAFERAATDDELAAMETELRRALDAGAAGFTTSRSASHQTPDDRPVASRAAPW
                     PEVQRLVGVLTDYPGRPFELALEPLAHDPDPSVRRESLGRLRDLAIDTAVPVMFGVLP
                     TTSQRYDELLALIDETTASGGRMYGQAAPRGVSMFISFETRTPFDRLPTWQVVRALPR
                     DAQVAELRKPDVRERLVAEAHGGTYGAGVGAELKPPNYDNTLVFDRVTPPH"
     assembly_gap    4261..4346
                     /estimated_length=86
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4347..4757
                     /locus_tag="PLV68_00815"
     CDS             <4347..4757
                     /locus_tag="PLV68_00815"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRD99804.1"
                     /translation="FYGVNPESDIERILTHPLTIMTFSDSGAHVSQICDSSIQTYLLA
                     YWVREREVLSLERAVRDMTLLPSMVWGFGDRGLVREGFVADLNVIDPNTVCPGYVDGL
                     LRHREENFAAIFRRRRHACSSPAPASDPRCSVVR"
     gene            4884..5486
                     /locus_tag="PLV68_00820"
     CDS             4884..5486
                     /locus_tag="PLV68_00820"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRD99805.1"
                     /translation="MARPKVPLISRRSVVVATLEILEHEGIDGLSVRRIARQLNVNAA
                     SLYHHFDSKDDILTAAARAALAEIQVDPFDPNDGSFVDWFAQVGLEHRRFLIARPYMI
                     PLMVQRVVPHTTLAAGHTGNVQLGRYGIADADDRIRIQDTIEGFVVGSALMASSGADR
                     EPSKTDEGDELAFLTVVKAMIESLIAFYKPTRSTAKSTRS"
     gene            5626..>5850
                     /locus_tag="PLV68_00825"
     CDS             5626..>5850
                     /locus_tag="PLV68_00825"
                     /inference="COORDINATES: protein motif:HMM:NF040248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRD99806.1"
                     /translation="MRKIALAFVQMVIVSFLVFLLLSASPGDPAQELAGEFATADQVA
                     KVRTELGLDRPVFVQYADWASDAVRGDLGRS"
     assembly_gap    5851..5871
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5872..>6244
                     /locus_tag="PLV68_00830"
     CDS             <5872..>6244
                     /locus_tag="PLV68_00830"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRD99807.1"
                     /translation="VSTLLARTAPISLSLVGGATVVAVFFAAILGIGAALRLGGFADR
                     TVLFLTSVGVAVPSFWLALILISTFSIRYGWFPALGYTPFSEGVMDWLDHLVLPSIAL
                     AGVTMAELTRQLRGSLADVMAT"
     assembly_gap    6245..6254
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6255..6574
                     /locus_tag="PLV68_00835"
     CDS             <6255..6574
                     /locus_tag="PLV68_00835"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRD99808.1"
                     /translation="SSRPKKVSSQLPQSSVIFKHGLKNALIPVLTLLGVRVSQLIGGS
                     VIVESIFNIQGLGQTVVRAALSRDIPVVLGITVFTSIIVLVISIALDLVHPILNPKLR
                     HHR"
     assembly_gap    6658..6672
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6673..>6948
                     /locus_tag="PLV68_00840"
     CDS             <6673..>6948
                     /locus_tag="PLV68_00840"
                     /inference="COORDINATES: protein motif:HMM:NF024313.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRD99809.1"
                     /translation="GGRTVRRFLASKAAVAGLVMIVVLIGIALFASVLAPHDPNFQNL
                     RGTLRSPSGDHWLGTDTLGRDVLSRLIYGTQISLLAGAEAVFVAFVLG"
     assembly_gap    6949..7017
                     /estimated_length=69
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7018..7499
                     /locus_tag="PLV68_00845"
     CDS             <7018..7499
                     /locus_tag="PLV68_00845"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRD99810.1"
                     /translation="ARAAASAPPLAVIAALGPGLVTAMFAIGLVYMPRFFRLTRATTH
                     DLNAEMFIRAERSLGARRTRVLFGHILPNAMPPLIVQVSLVFGTAILAEASLSFLGLG
                     VKAPTASWGSMLADASTRLDKSYLIWGPGIALTITVLAFAMVGDGLRDALGIGRTVK"
     gene            7499..>9076
                     /locus_tag="PLV68_00850"
     CDS             7499..>9076
                     /locus_tag="PLV68_00850"
                     /inference="COORDINATES: protein motif:HMM:NF008453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRD99811.1"
                     /translation="MNNTAGAEAVLAIDDLAVGFPIGGRIVNVVESASLTVEQGAIVG
                     LVGESGSGKTVSAMTAAGLLRALGGTVTRGSISFLGQDVTNLSERDWVRIRGRDIGVV
                     FQNPTRTLNPAFRIGEQLAEVVRRHEGVSRSAAWKRAVEMLDRVGIADAERRARDYPH
                     QFSGGMCQRVAIAMAIICHPKLLIADEPTTALDVTVQRHILDLLLELRRESGVSILLI
                     THDLGVVAHTCDDVAVMYAGQVVERGKVHPLFASPQHPYTTGLLNALPNGSVGGLPTA
                     IPGQVPAPTRMPTGCRFAPRCGHAEHRPSRTRRGARPVVVAVHQDSRPGEGVVVSLLE
                     VEALRKTYPVRHAEPFIAVDDVSFTLEAGQTLALVGESGAGKSTTGSLVLRLIEPDSG
                     SVRLQGRDVLALNREELRGFRQNMQMIFQDPQSSLDPRLSVGVSVSEPLKIHFGMNKV
                     DRQRRAMELFAMVGLGRDTVRKFPHELSGGMLQRAAIARALTVSPHLLVCDEAVSALD
                     VSVRAQVLNLLVELQNEL"
     assembly_gap    9077..9212
                     /estimated_length=136
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9523..9543
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9544..>10788
                     /locus_tag="PLV68_00855"
     CDS             <9544..>10788
                     /locus_tag="PLV68_00855"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRD99812.1"
                     /translation="KSSTTSTTAATSTTASTTDSTAAPSTSTGTETSQSATPQTDPPP
                     AYDQSASLHATYYIAPTHLDPHNETNGGLRVFWRPAYDTLFENYSGGTHPGVALSVDR
                     SDDGLTVTLNLRDDVLFADGTPLESDAVVGSLERVRAEGAPVITATLKAVTEIAAVDA
                     HTVEIQLSEPLPQLEALLGGPAGAIISPAGLAAPDQLATQTFGSGPFQSSDFVPDDHV
                     TYTRSDVDYWDPAAGLYSELTITFVSELQTNLNGLQSGDFDIPFAVGGGDQLQRVADA
                     NGFVVYDYSSGGVNTYFLRTTRSGLIDQRVRQALNYAIDREGVAIGIFPGGDCVATSQ
                     NFPVGHPMHLAEEPYPLDTDKAKELIAEAGAEGMQLELIDGGNSGQHLIGLALQQIWN
                     DLGLDVTVTTNPSGAAAAFGEGG"
     assembly_gap    10789..10830
                     /estimated_length=42
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10831..11124
                     /locus_tag="PLV68_00860"
     CDS             <10831..11124
                     /locus_tag="PLV68_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99813.1"
                     /translation="LAIERYYTPGGFFHLADQDSAAIDELLAEIKSTPPDQAAPLYAE
                     MGQLLADGAYTVPVCEPENHMVARPEVGGLDDKRAGEMSGNQPDYRFLWVAAD"
     assembly_gap    11194..11221
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            11244..>11563
                     /locus_tag="PLV68_00865"
     CDS             11244..>11563
                     /locus_tag="PLV68_00865"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRD99814.1"
                     /translation="MTAGHRASWLLAEYGADVVIVEPVGGDGRRDAVEFAVFNRGKRS
                     VELDPDDGDTISRLVEDADVVVVSAGDAMAGWQARDVRRLHELHPRQVFLAMSTAGLD
                     GPLA"
     assembly_gap    11564..11722
                     /estimated_length=159
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            11754..>11935
                     /locus_tag="PLV68_00870"
     CDS             11754..>11935
                     /locus_tag="PLV68_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99815.1"
                     /translation="MVETSLLDGVLAYLSMYWGESDAAPGGDSSSAMRSTGATATTRV
                     ATGAYRCSDGEYIGMST"
BASE COUNT         1813 a         3668 c         3860 g         2007 t
ORIGIN      
        1 gccggcgtcg cgatcgagcg tgcccgtcgc ggtgacggac cgtcgtttgt cgaggcggtc
       61 agcgtgcgca accggggtca tttcaccggc gatctcgaac cgtaccgacc gctcgaccgt
      121 cagctggctg acccgatcca gatcgcggcc gctcgactcg tcgcgactgg cgcggcgcag
      181 gcagaactcg accgcatcaa cgccgacgcg gccgccgaaa tggaagcggc actcgcggac
      241 gccaaggccg ctccgctgcc cggtcgtgat cgtctgctcg ccggagtctg ggcatgaacg
      301 cgggtacgcc aagcacggcc gaaatggtgg agatgtcgta tgcgcgggcg atcaacgccg
      361 cgctggccga agagatggag cgggacgagc gggtgttcct gatgggccag gacgttggca
      421 agctcggcgg tgtcttcggc gtgaccaagg gcctacagca gcgcttcggc ccgcagcggg
      481 tgatcgacac cgcgatcgtc gagcacgcga tcgtcggcgg tgcggctggt gcggcgatgg
      541 ccggaatgcg cccggtcgcc gaggtgcagt tcgcggactt cctgcttctc gccggtgacg
      601 agacagcgag caagctcggc aagtggcggt tcatgcacgg cggacgactg accgtgccgg
      661 tggtggtgcg tgcgccgatg ggtgcccaag gcggtgtcgg cgccgagcat tcgcaatgcc
      721 tcgaaggcgt gttctggcac acgggagggt tgcggatcgt ggtgccctcc acccccgccg
      781 acgccaaggg tttgctcaag acggcgatcc ggagcgacga tccggtgctg ttcttcgaac
      841 accggcggct gtatcgtgca cgcggaccgg tgccggtcga cccggacgtg gtcgtgccct
      901 tcggccaggc ggccgtgcgc cgttctggtc gcgacgtcac cttggtgacg tggtcggcgc
      961 tggtcgagga ctgtgtgcgc gccgccgagc aactggccga gcgcggtgtg tcggccgagg
     1021 tactcgacct gcgcacgctg cagccactcg acaggacgag cctggctgag tcgatcgggc
     1081 gtacggggcg ggtgatcgtg gtccacgaag cggccaagac cgcaggtccc ggtgccgaga
     1141 tcgcggcgat cgtggccgag gagttctttc cgctgctgcg ggctccggta cggcgggtgt
     1201 gcggctacga cgcgccggtt ccgcaatcag gcgtactgga ggcgatgtgg ttgccgagtg
     1261 tggacgagat cgcgacggca gcagaggagt tggttgcatg gtgaccaggt cggttcggct
     1321 gccgttgatg ggggagaccg tcaccgaagc ggtgatcgtg cgatggttgc gccgcgtcgg
     1381 cgagcctgtc gtcgagggcg aggcgctgtt cgaggtctcg accgacaagg tcgacaccga
     1441 ggtgcccgcg ccgttgagcg gcacgctgac cgggattctc gtcggtgaag ggatgaccgc
     1501 gacgatcggt caggtgatcg ccaatgtcga gaccaccgat accggcgaca cgctcgacgg
     1561 gcccgacgtc gacgacggat cggtcgctgc aggcgggttg gtggcagagc gtgccgggct
     1621 cgtggaggag ccgcctgcgg ccaacggtgc acctcgggtg acgccactgc cgccgcgccg
     1681 gtcccagccg gagcttcgtc cctcgcccga gcgtcgcccg agcgcctcga gctccgcgtc
     1741 ggcagccgtg acgccacctg ccgtgtcgga tgagcccgct tgggtgccat tcagcccgat
     1801 gcgacgcgtg atcgcggccc gcatgaccga gtcgttgcac gtggccgcgc acaccctcgt
     1861 ggtgatggag gccgactacg agaacgtcgc ccgcgcccgc gcagagcggc ctgggttgac
     1921 gtatctgccg ttcgtgtgcc gtgcgatcgt cgatgcgctg cgcgacttcc cactggtcaa
     1981 tgcccacgtc tccgacgacg gtctgcgtcc gtttgacgtg gtcaacctcg ggatcgctgt
     2041 cgatctcgac ctcgacggac tcgtggtgcc ggtggtgcag caggccgagc ggttgcggct
     2101 cagtggcatt gcggccgcga tcaccgaggt cgccgggcgg gcgcgggcgc gttcactgcc
     2161 ctcgcaggcc tacgacggcg gcacgatcac ggtcaccaac gccggcggcc gcggcagctt
     2221 gtttggacgg ccgatcatca acctgccaca agcggcgatc gtcgagaccg atgcagtcga
     2281 agagcgcctg gtcggcaccc gcgaccagtc cggggcgctc gtggtacaac tgcggcggcg
     2341 gggctacctg tcgatgtcgt acgaccaccg tgccttcgac ggggcatacg cctgcgggtt
     2401 cctcagtgcc gtctgccgca cgatcagcga gcgggactgg gcagcagagc tgtagctgac
     2461 cagacctcct cgcgagtgac ctactcgcga tagcccagct tgtgccagcg cagctgatcg
     2521 tccaacgcct tgccgctcgg ggtcagcacc ggttcagtcg tcaacaacaa gtcaccgtcg
     2581 atccacgaga cgcgccgcac cagatcggtg ccgacgtgtt cgggccaggc cgagatgcgc
     2641 acgtggtgcg tcacgacgtc accgtccaac gtgtacgagc cgccgtaggc cgaaaacagc
     2701 gccgcggctg ccgcctggtc ggccggactg ccatcgcgga agttgacgcc agcaggccaa
     2761 ggcctgtctg ggtcggtgag aatcgccgac atgtggccgt cggcttgata cgaaatcagg
     2821 cccgacggat gctcaccaag catgtggtgg cgcggctcgc cgtcgatgcg gcgaacgaac
     2881 gacaccaacg tccaggtgcc cagcagtgca tgcgagtcca tcgcgattcc cctctcgatc
     2941 gtgcaggcga cagcctctcg gttccggtgg cgccctcggg cgacgatcgt accgcgttga
     3001 tcaccgtaac cgaacaccgt atgatagccg ccgcaacgcg gacttccggc cgagtggccg
     3061 gagtaccggg actcggggaa ccggcaggcc gaaggggggc gcacgatgag ttttgacttg
     3121 atcatcagag gcggagaggt ggtcgacggc agcgggctac cgcgctatcg ggccgatgtc
     3181 ggcgtgaccg atggacggat caccgcgatc ggacgcatcc gcgagcgtgc ggcgcgagag
     3241 atcgatgccg aaggccacgt cgtcacacca ggcttcatcg acgggcacac ccacatggac
     3301 gcacaggtga tgtgggaccc gctcgggacg tgctcgagcc tgcacggggt gacgaccgcg
     3361 gtgatgggca actgcggctt cacccttgcc ccgtgccggg atggtgccga cgggcgggag
     3421 ttggtcgtgg ccaacatcga gcgagccgag gacatctcgg ccgaggcgat ggccgccggc
     3481 atcgactgga cgtggtcgaa cttcgacggc tatctcgacg cggtcgggtc gcggcagttc
     3541 gcgatcaact acgcggccaa catcggccac agcgcactgc gcacgtgggc gatgggagcc
     3601 gccgccttcg agcgtgccgc gaccgacgac gagctggcgg cgatggagac cgagctgcgg
     3661 cgcgcgctcg acgccggggc agcggggttc accacgtcgc gttccgcctc ccatcaaacg
     3721 ccagacgatc gtcccgtcgc ctcgcgggcc gcgccatggc cagaagtgca gcgtctggtc
     3781 ggtgtgctga ccgactaccc gggccgcccg ttcgagctgg cgttggaacc gttggcccac
     3841 gacccagacc ccagcgtgcg ccgcgaatcg ttggggcggt tgcgcgacct ggcgatcgac
     3901 accgcggtgc cggtgatgtt cggcgtgcta ccgaccacca gccagcgcta cgacgagctg
     3961 ctcgcgctga tcgacgagac cacggcctcg ggtggccgca tgtacggcca ggctgctccg
     4021 cgtggggtgt cgatgttcat cagcttcgag acccgcaccc cgttcgaccg gctgccgacg
     4081 tggcaggtgg tccgtgcgct gccgcgcgac gcccaggtag ccgaactccg caagcccgac
     4141 gtccgtgagc gccttgtcgc agaggcacac ggcggaacct acggcgccgg cgtcggtgcc
     4201 gaactgaagc cgccgaacta cgacaacacc ctcgtcttcg atcgggtcac cccgccgcat
     4261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnttct acggcgtcaa ccccgagtcg gacatcgagc
     4381 ggatcttgac ccaccccctg acgatcatga ccttctccga ctccggcgcc cacgtcagcc
     4441 agatctgcga ctcgtcgatc cagacctatc tgctggccta ctgggtccgc gaacgcgaag
     4501 tgttgtcgct cgagcgcgct gtgcgcgaca tgacgctgtt gccatccatg gtgtggggct
     4561 ttggcgatcg cggtttggtg cgcgaggggt tcgtggccga tctcaacgtc atcgatccga
     4621 acaccgtctg ccccggctat gtggatggac tgctgcggca ccgggaggag aacttcgccg
     4681 cgatcttccg gcgcaggcga cacgcctgtt ccagtccagc accggcatcc gatccacggt
     4741 gctcggtggt gaggtgacct tcagcgatgg cgagcacacc ggggcgctgc caggacgact
     4801 ccttcgccgc cgatgacatg accccgtctg gcgttggcac ggccgagtag ggtcgggcgc
     4861 cgaactgcac ttggagttga tgcatggccc ggccaaaagt acccctcatt tcacgacgca
     4921 gcgtcgtggt cgcgacgttg gagattctgg aacacgaagg gatcgacggg ttgagcgtgc
     4981 ggcgcatcgc ccgccaactc aacgtcaacg cagcatcgct gtaccaccac ttcgactcga
     5041 aggacgacat tctcaccgca gcggcgcgtg ccgcgctggc cgagattcag gtcgacccat
     5101 tcgatcccaa cgatggcagt ttcgtggatt ggttcgctca ggttgggttg gagcaccgac
     5161 ggttcctgat cgcccgtccg tacatgattc ccctcatggt ccagcgggtc gtgccgcaca
     5221 ccacgctggc tgccggtcac accggcaacg tgcagctcgg tcggtatggc attgccgatg
     5281 ccgacgaccg cattcgcatc caggacacca tcgagggatt cgtcgtcgga tcggcgttga
     5341 tggccagttc cggggccgat cgcgagccga gcaagacgga cgaaggggat gaactcgcgt
     5401 tcctgacggt ggtcaaggcg atgatcgaga gcctgatcgc cttctacaag ccgacgcgaa
     5461 gcacagccaa gagcacccgg agctgacgac acgggccctg agagccccca aaaaagagcg
     5521 acttgcccat ttcgtcaatc gctgttagat tagtggggcc ttgaatctcg tggtcgaggt
     5581 atgtcggacc tcctgggggg tgctgtggcg atagtcctcg ccgtcttgcg caagattgct
     5641 ctggcttttg tccagatggt gatcgtgtcg ttcttggtgt tcctgctgct cagcgcgtca
     5701 cctggtgatc cagcccagga actggctggt gagttcgcga ccgccgacca agtggccaag
     5761 gtgcgcactg agctcgggct tgatcgcccg gtcttcgtgc agtacgcaga ctgggcctct
     5821 gatgcggtac ggggcgatct cgggcggtcg nnnnnnnnnn nnnnnnnnnn ngtgtcgacg
     5881 ctgctggccc gcacggcacc gatctcactc agcctggtcg gcggcgccac cgtggtggcg
     5941 gtgttcttcg cggcgatcct cggtatcggg gcggcgttgc gtctcggcgg cttcgccgat
     6001 cgcaccgtgc tgttcctcac cagtgtgggc gtcgcggtgc cgagcttctg gctggccttg
     6061 atcttgatct cgaccttctc gattcgatac ggctggttcc cggcgctcgg gtacaccccg
     6121 ttcagcgagg gggtgatgga ttggctcgac catctcgtgt tgccctcgat cgccctcgcg
     6181 ggggtgacga tggccgaact gacacgccag ctgcgcggtt cgctcgccga cgtcatggcg
     6241 acggnnnnnn nnnncgagct cgcgaccgaa gaaggtgtca agccagctgc cccagtcgtc
     6301 ggtgatcttc aagcacggac tcaagaacgc gttgatcccc gtgctcaccc tgctcggtgt
     6361 acgcgtctcg cagctcatcg gaggctcggt gatcgtcgag tcgatcttca acatccaggg
     6421 actgggacag acggtggtgc gggccgcgtt gtcgagagac attcccgtgg tcctcggcat
     6481 caccgtgttc acctcgatca tcgtgttggt gatcagcatt gccctcgacc tcgtgcaccc
     6541 gatcctgaac cccaagctga ggcaccaccg atgagcacgg acaacctggc caccgacgat
     6601 ctggcgcctg acgatgtgac aactgacgag tccgtcgccg cgcttggcga tgccgacnnn
     6661 nnnnnnnnnn nnggcggacg cacggtgcgc cggttcctgg cgtcgaaggc ggcggtcgcc
     6721 gggttggtga tgatcgtcgt gctgatcggc atcgcgctgt tcgcgtcggt gctggccccg
     6781 cacgacccca actttcagaa cctgcgcggc acgctccgct cacccagcgg cgaccactgg
     6841 ctgggtacgg acacgctcgg tcgcgacgtg ctgtcgcggc tgatctacgg cacccagatc
     6901 tccctgctcg ctggcgccga ggcggtgttc gtggcatttg tcctcggcnn nnnnnnnnnn
     6961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnatg
     7021 cgcgtgccgc cgcttcggct ccgcctctcg cggtgatcgc ggccctcggc ccagggctgg
     7081 tcacggccat gttcgccatc gggttggtct acatgccccg gttcttccgt ctcacccggg
     7141 caaccacgca cgacctgaac gccgagatgt tcatccgggc cgaacgcagc ctgggtgcgc
     7201 ggcgtacacg ggtgctgttc ggccacattc tgcccaacgc gatgccgccc ttgatcgtgc
     7261 aggtcagcct ggtgttcggg accgcgatct tggctgaagc gagcctgagc tttctcggtc
     7321 tgggtgtcaa ggccccgacc gcgagttggg ggtcgatgct ggccgacgct tcgacccgac
     7381 tcgacaagtc gtatctcatc tggggtccag ggatcgcctt gacgatcacg gtcctggcgt
     7441 tcgcgatggt cggcgacggc ttgcgtgacg cgctgggcat cggaaggacg gtgaagtgat
     7501 gaacaacact gctggtgccg aggcagtgct cgccatcgac gatctcgccg ttgggtttcc
     7561 catcgggggg cgcatcgtca acgtcgtcga gtcggcttcg ctcacggtgg aacagggagc
     7621 catcgtgggg ctggtcggcg agtcaggctc gggaaagacc gtctcggcga tgaccgcggc
     7681 cggtttgctc cgcgcgctcg gcggcacggt cacccgcggc tcgatcagct tcctcggcca
     7741 ggacgtcacc aacctgtccg aacgcgattg ggtccggatt cggggacgcg acatcggtgt
     7801 ggtcttccag aacccgacgc gcacactcaa tccggcattc cggatcggcg agcaactcgc
     7861 cgaggtcgtg cgacgccacg agggtgtctc tcgttcggcg gcgtggaagc gcgcggtcga
     7921 gatgctcgat cgtgtcggga tcgccgatgc cgagcgccgc gctcgtgact acccccacca
     7981 gttctcgggc ggcatgtgcc agcgggtcgc gatcgcgatg gcgatcatct gccatccgaa
     8041 gttgctgatc gcggacgagc ccaccacggc gctcgacgtc acggtgcagc gccatatcct
     8101 cgacctgctc ttggagttgc gtcgcgagtc cggcgtctcg atcttgctca tcacccacga
     8161 cctgggcgtg gtcgctcata cctgcgacga cgtcgcggtg atgtacgcgg gccaggtggt
     8221 ggaacgaggc aaggtccacc cgttgttcgc ctcgccacag cacccgtaca cgacggggct
     8281 gctcaacgcg ctccccaatg gctccgtcgg tggactgccc acggccatcc cgggccaagt
     8341 gccagcgccg accaggatgc cgaccggttg tcggttcgca ccgcggtgcg gccatgccga
     8401 gcatcgcccc agtagaactc gtcgaggtgc acgaccggtc gtggtcgcgg tgcatcaaga
     8461 ctcccgacct ggagaaggtg ttgtcgtgag cctgctggaa gtcgaagctc tgcgcaagac
     8521 gtaccccgtg cgccatgccg agccgttcat cgccgtcgac gacgtctcgt tcacgctgga
     8581 ggccgggcaa acactggcgt tggtcggcga gtcgggtgct ggtaagtcga ccaccggatc
     8641 actggtgttg cggttgatcg agcccgacag cggatccgtt cgtctccagg gcagggacgt
     8701 gcttgcactc aaccgtgaag agttgcgcgg ctttcgccag aacatgcaga tgatcttcca
     8761 agatccgcag agttcgctcg acccgcggct gagcgtcggc gtgtcggtgt ccgagccgct
     8821 caagatccac ttcggcatga acaaggtcga tcggcagcgc cgcgcgatgg agctgttcgc
     8881 gatggtcggc ctcggccgcg acaccgtgcg caagttcccc catgaactgt cgggcggcat
     8941 gctccagcgc gccgcgatcg cgcgagcgct cacggtcagt ccgcacctgt tggtgtgcga
     9001 cgaggccgtg tccgcgttgg acgtgtccgt gcgggcgcag gtgctcaacc tgctggtcga
     9061 gctgcagaac gaactgnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncgcgagtt gctctccgct gttctgcccc
     9241 ctgacccgac gatgcgtggt ccagttgccg ataaccaacc tttgttagag aacatcatcg
     9301 cctagcgaca ggtttcgcct gttgtagctg ggttttacag ccaaacaggt attgattcag
     9361 gtcggtcact gaggtacgat gccgccatcg aagtgaggct tcgggctgcc cgctcagtcg
     9421 ggtaggggaa accaaggggg agatcatgaa gatccgatcg ggtcatactc atttctcagt
     9481 aagtaaacga cgtacggtta cgggtgtcgc gctcggcgtg gcnnnnnnnn nnnnnnnnnn
     9541 nnncaagtcg agtacgacat cgaccactgc tgcgacaagt acgactgcgt cgacgacgga
     9601 cagcacggca gcaccgtcga cgtcgacggg gaccgagacg tcgcaaagcg cgacgccgca
     9661 gaccgatccg ccgccggcgt acgaccaatc ggcgtcgctg cacgccacct attacatcgc
     9721 gccgacccac ctcgatccgc acaacgagac caacggaggt ctgcgcgtct tctggcgtcc
     9781 ggcctacgac acgctgttcg agaactacag cggtggcacc catccagggg tggccctctc
     9841 cgtcgaccgc tcagacgacg gcttgaccgt cacgttgaac ttgcgcgacg acgtgctctt
     9901 cgccgacggc acaccgttgg agtccgatgc ggtcgtcggc agcctcgagc gtgtgcgggc
     9961 cgagggcgcg ccggtgatca cggccacgct caaggcggtg accgaaatcg cggccgtcga
    10021 cgcccacacc gtggagatcc agctctccga gccactgccg caactcgaag cgttgctcgg
    10081 tggccctgcc ggggcgatca tcagccctgc gggtctggct gctcccgacc agttggcgac
    10141 gcagaccttc ggctccgggc cgttccagtc gtcggacttc gtacctgacg accacgtgac
    10201 ctacacccgt tccgacgtcg actactggga tcccgcggcc gggctctact ccgagctgac
    10261 gatcacgttc gtctcggagc tgcagaccaa cctcaacggt ctccagtcgg gtgacttcga
    10321 catcccgttt gccgtcggtg gtggcgatca gctgcagcga gtggccgacg ccaacggatt
    10381 cgtggtctac gactacagct cgggtggtgt caacacatac ttcctccgca cgacccggtc
    10441 gggtctcatc gaccagcgtg tgcgtcaggc gctcaactac gcgatcgatc gtgaaggggt
    10501 tgcgatcggc atcttccccg gtggcgactg cgtggcgacg agccagaact tccctgtcgg
    10561 ccacccgatg caccttgccg aggagcccta tccgctggac accgacaagg caaaggagtt
    10621 gatcgccgaa gcgggtgccg aaggtatgca actggagttg atcgacggtg gcaacagtgg
    10681 tcagcacctg atcggacttg ctctccagca gatctggaac gatctgggtc tcgatgtcac
    10741 ggtgacgacc aatccttccg gagcggcagc agccttcggt gaaggtggnn nnnnnnnnnn
    10801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ctggccatcg agcgctacta caccccgggt
    10861 ggtttcttcc accttgctga ccaggattcg gctgcgatcg acgaactgct ggccgagatc
    10921 aagtcgacgc cacccgacca ggcggcgccg ctgtacgcgg agatgggtca gctgctcgcc
    10981 gatggtgcgt acacggttcc ggtctgcgag ccggagaacc acatggtcgc tcggcccgag
    11041 gtcggtggcc tcgacgacaa gcgggcaggc gagatgagcg gcaaccagcc ggactatcgc
    11101 ttcctgtggg tcgctgccga ctgacggctg aacggctggt tgatgtgacg gtcccccgag
    11161 gccctgctgt tctggtgcac gcatccgaac agcnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11221 nccgcgtcgt ggacctggcg gagatgacgg ccgggcaccg ggcctcgtgg ttgctggccg
    11281 aatacggtgc cgacgtcgtc atcgtcgaac cggtgggcgg tgacgggcgg cgggacgcgg
    11341 tcgagttcgc ggtcttcaat cgtggcaagc gcagcgtcga gctcgacccc gatgacggcg
    11401 acaccatctc gcggctcgta gaggacgcgg acgtggtcgt cgtctccgcc ggggacgcga
    11461 tggccggttg gcaggcgcgg gacgtacgcc ggctgcacga actgcatccg cgccaggtgt
    11521 tcttggcgat gagcaccgcc ggactggatg gaccgctggc cgannnnnnn nnnnnnnnnn
    11581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11701 nnnnnnnnnn nnnnnnnnnn nngttccgtc gggcgcgtga tggacgtggc cgtgtggtgg
    11761 agacatcgtt gctcgacggg gtgttggcct acctgtcgat gtactggggg gagtcggatg
    11821 cggcacccgg tggtgactca tcgtccgcga tgcgctcgac aggtgccacc gcaaccaccc
    11881 gtgtcgccac cggtgcatac cgttgctccg acggcgagta catcggcatg tcgac
//
LOCUS       DAORNF010000010        11594 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_4198_length_11594_cov_1.682122, whole genome shotgun sequence.
ACCESSION   DAORNF010000010 DAORNF010000000
VERSION     DAORNF010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 11594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11594
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4198_length_11594_cov_1.682122"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="PLV68_00875"
     CDS             <1..594
                     /locus_tag="PLV68_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HRD99816.1"
                     /translation="VPYLPIDPKHVGRTYEAVIRVNSQSGKGGVAYLMKTEHGFDLPR
                     RMQIEFSKTIQHITEDTGTEISPVVMWDTFQQQYLPDEPRIRLISHELRSDSVGKSTD
                     ITAQLTVDGEHITVTGSGNGPIDAFVTALNASLGTEIDVVDYAEHAMGAGKDATAVAY
                     VEARDGDGQVRWGLGTDPNMLTASLHAVVGAVVRHTN"
     assembly_gap    798..833
                     /estimated_length=36
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <834..1056
                     /locus_tag="PLV68_00880"
     CDS             <834..1056
                     /locus_tag="PLV68_00880"
                     /inference="COORDINATES: protein motif:HMM:NF018595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HRD99817.1"
                     /translation="GPTAARKGDPMKVHVNFDVCASTASCMQTAPEVFEVRADGYLYV
                     LQEEPGEELREAVTDAADLCPTGAITIEG"
     assembly_gap    1189..1293
                     /estimated_length=105
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1294..1824)
                     /locus_tag="PLV68_00885"
     CDS             complement(<1294..1824)
                     /locus_tag="PLV68_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99818.1"
                     /translation="MTSARFDVVVIAQSAADRLGVVARRARVLARGALLFALAGGVLA
                     YAVGWFAFPSSWHAAWGIVGLVICAVPGLIVWLAIRRVRRVSATLPATAVELQAALND
                     PRLRESVYNLVERDGDHEERTPLIKLGKELNELRTAADAHRATLTNAWASITSLTSLP
                     GLVALATLGTIALVTPL"
     gene            1931..>2251
                     /locus_tag="PLV68_00890"
     CDS             1931..>2251
                     /locus_tag="PLV68_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99819.1"
                     /translation="MLTSLSRSARVRNWTAGLQRLTDSLGVQRRMAVAIGLSGLTGGV
                     IGAAYLTVLDVTNRAIGPGRWSPGPHLVILALVGVTVAALVKWLGPPANVELLVDNIH
                     VPGMS"
     assembly_gap    2252..2294
                     /estimated_length=43
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2295..3232
                     /locus_tag="PLV68_00895"
     CDS             <2295..3232
                     /locus_tag="PLV68_00895"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HRD99820.1"
                     /translation="LLCTGAGGTLGPEAPLVTTTGTIAHRQASRIGLDRGDIRVVSIA
                     GMSAGFAVLFGAPLGGAIFALEIPHRRGIGYQEAAIPACFGAIIGHACSTAAGHMGLE
                     PIWDFPMINHLKWLDLPWSLLAGVIGAAIAVVFTVLVRAMAKVMRTTPLPSRPVIGGL
                     VLGGIALVSPYVLTNGELQIDHLAKTAGVAALLVAAGVKLVGAATCVATGWQGGFIIP
                     LFFAGFCVGFALAPHLPGASVWPFVIAVMISANVGVTKTPLGSALVVTEMAGAVMLPT
                     ALIAALTSLVLTSQVHLIESQRDRVDVYGRRHDET"
     gene            3219..3647
                     /locus_tag="PLV68_00900"
     CDS             3219..3647
                     /locus_tag="PLV68_00900"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRD99821.1"
                     /translation="MTRRERALNNAEYRSLAQFRYALRQFLAFSADAARAEGLTSAQH
                     QLLLTVRGAEADKRQPSMSDVAEHLQLKLHSTGELVARAVEAGLVERVTDPADARRVL
                     IATTAAGRGHLERLSVLHRAELRRFRTEMNALLRAIDTPT"
     gene            complement(3782..4618)
                     /gene="pyrF"
                     /locus_tag="PLV68_00905"
     CDS             complement(3782..4618)
                     /gene="pyrF"
                     /locus_tag="PLV68_00905"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HRD99822.1"
                     /translation="MSFLRRLDAAWATQDSMLCVGIDPDPALMPPHLATRPDGIETFC
                     REIIDATAPFACAFKPQIAYFAANRAEAVLEALGAYVAMAALISIFDPYIGPTAAQYA
                     REAFDRYGAHAVTVNPYLGTDSVEPFLAHAGRGVILLCRTSNAGGDDFQSLVLDGRPL
                     YCHVAERVARQWSQIGECGLVVGGTYPGELATVRDIVGDLPILVPGIGAQGGDIAATV
                     AAGASTSGRGLLISSSRAILYASQGTDFAAAAATEARKTRDAIRTARRDGVRLTPVRG
                     VD"
     assembly_gap    4784..5013
                     /estimated_length=230
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5246..5935
                     /locus_tag="PLV68_00910"
     CDS             5246..5935
                     /locus_tag="PLV68_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99823.1"
                     /translation="MASHRLGLALIGMPRYQSNLVEVTSGGLHRIPGVVTHRSLRPLP
                     HTTVRGVPTTAPARTLIDVGCLVSQALLARWTTTWVSERVVTVTELESEMAAARGHRG
                     VPLLRAVLDDLDRRHEVADSPVEAKLGMLLEDNGFPPLTLHHVVTVSSGAEFELDWSY
                     PQWQIGFEVDGYGVHLRSHESFDHDRFRRNELVIDGWTILNFSGRQVARRSRTVLDQA
                     RRALTERSLLP"
     gene            complement(6053..>6962)
                     /locus_tag="PLV68_00915"
     CDS             complement(6053..>6962)
                     /locus_tag="PLV68_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRD99824.1"
                     /translation="AKPSLRVINVARGGIVDEDALADAIRDGVVAGAALDVFIKEPVT
                     ESPLFGLDSVVVTPHLGASTREAQDKAGDTIADMVQLALAGDFVPFAVNVNAAEANET
                     IKPFLPVAERVGSLFASLVGASPSTLEVRSEGELAGYDTRILTLAVLKGFYAARSAEP
                     VTYVNAPQLAKSNGVEVREVSSTATTDYVNLITVAGAGHSMSATLSGRRGEPRLVSID
                     DHQFDIPPADHMLMVTNDDRPGVIGVVGTLLGNAGINIADMDVGQAKEPGTAVMLIAP
                     TAEVPTSTVESLRAAPGIIAVATLHG"
     assembly_gap    6963..6972
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6973..7620)
                     /locus_tag="PLV68_00920"
     CDS             complement(<6973..7620)
                     /locus_tag="PLV68_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRD99825.1"
                     /translation="MSRILVTEEIAEGGLDRLRGAGHVVDVELDLTKLPSAIKGAHAL
                     IIRSATQVDAALLELADELMVVGRAGIGLDNVDVEAATRRGVMVVNAPQSNIVSAAEH
                     TMALLLSQARNVPQAHAALKAGRWERSKWEGVELADKTLGIVGLGRIGKLVADRAKGF
                     GMRLVAFDPYVAPDRARTMGIEMMDLDQLVAESDFLTIHLPKTKDTTGIIGRDLLL"
     gene            7714..>7958
                     /locus_tag="PLV68_00925"
     CDS             7714..>7958
                     /locus_tag="PLV68_00925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99826.1"
                     /translation="MAEQGVTGAHDLDRIERWFIDRGVPHFIESYEARSDIWTRAIPI
                     LLVAYFVGGFQALDIRGWSLAKNLGAAAIVVAVLMLS"
     assembly_gap    7959..7969
                     /estimated_length=11
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7970..8901
                     /locus_tag="PLV68_00930"
     CDS             <7970..8901
                     /locus_tag="PLV68_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99827.1"
                     /translation="ALRRRWSSMPREIGHVELAAFVIGPSIPSALFGQWGDALQAALI
                     GVAVLAVIYLGTSYAVIPMMRWAMHRAAGQVRSIANLIVRALPLLLLFATFLFINAEV
                     WQVAGALDGPAYWLTLAIFFALGALFAISRVPGLMQDLARFGTWTDVAALVDGSPAAG
                     LDTTPADGCPEPTTLGRRQRFNIALLMLFGQGLQITLVALMLTGFFVLLGFLAISEQT
                     ALSWTTLSDVHVFARLHLGQRELVLSGPLLRVAGFLGTFSGMYFTVVSATDATYRDEF
                     AEDVRPEVQRIFAVHAVYRCALRAKAAATEPFR"
     gene            8913..9335
                     /locus_tag="PLV68_00935"
     CDS             8913..9335
                     /locus_tag="PLV68_00935"
                     /inference="COORDINATES: protein motif:HMM:NF014132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HRD99828.1"
                     /translation="MAFQFLSDAWMVEARAIRAKYEGEVPPVTQSIRINLVITDAPFG
                     DDETASQVDAFIDTSGGSMVMDLGTLDQPDTTITTDYPTAKALFVDQDAAAAMQAFMA
                     GKIRVQGDMTKMMALSTAVPASEATQQIAEEIKAITAD"
     gene            complement(9366..10241)
                     /locus_tag="PLV68_00940"
     CDS             complement(9366..10241)
                     /locus_tag="PLV68_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRD99829.1"
                     /translation="MGSLEGRVAIITGAGRGIGREHALLFASEGAKVVVNDLGGANDG
                     TGTDLTPAQQVVAEIEALGGEAIVNGDNVADWQGAQRLINSAIEAFGDLDILVNNAGI
                     LRDRVLVNMTEEEWDAVIAVHLKGHFAPTRWAAAYWREQTKAGIDKPRNIVHTSSTSG
                     LFANPGQANYGAAKTGIATFSQICAKELVRYGVKSNCISPGARTRLTLATPGLEDIMN
                     PRDGAFDMWDPANISPLVAYLATAECAFTGETFFVQGGRVTRVDSWAMGDTVERDSRW
                     TVSELADALAPLVRD"
     gene            complement(10365..>11155)
                     /locus_tag="PLV68_00945"
     CDS             complement(10365..>11155)
                     /locus_tag="PLV68_00945"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HRD99830.1"
                     /translation="VAGVTLAEGGTILFVVSEGPNLSTLPDVTGMELAVAEQTLTAAK
                     LQMRVDSEQFCESAPPASGLAAASITCPDVPAGSVISWRYEAQPNTTAGAEVMPGSVV
                     AVVVSKGPAQRLMPELGMLDEVTATQLLAALGLVGAKSDDIFSLDVPAGQVAWQQFPM
                     DQPLNKGDTVSFALSKGPEIVAIPTIEGQTHQQLVDAIAVVGLVVGAVTGPTDGSGTL
                     QSVTSGGQPVASGQPLIKGSVIDIAYNDPPPPPPPSTDAPPTDG"
     assembly_gap    11156..11326
                     /estimated_length=171
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1783 a         3720 c         3684 g         1801 t
ORIGIN      
        1 gtgccgtacc tgccgatcga ccccaaacac gtcggccgca cgtacgaggc ggtcatccgc
       61 gtcaacagcc agagcggcaa gggcggcgtc gcctacctga tgaagaccga gcacggcttc
      121 gacctgccgc ggcggatgca gatcgagttc agcaagacca tccagcacat caccgaggac
      181 accggcaccg agatcagccc ggtggtcatg tgggacacgt tccagcagca gtacctgccc
      241 gacgagccac gtatcaggct gatcagccac gagctgcggt cggacagcgt cggcaagtcg
      301 accgacatca ccgctcagct caccgtcgac ggcgagcaca tcacggtcac cggctcgggc
      361 aacggcccga tcgacgcgtt cgtgaccgcc ctcaacgcgt cgctcggcac cgagatcgac
      421 gtggtcgact atgccgaaca tgcgatgggc gcgggtaagg acgccacggc cgtggcctat
      481 gtcgaagcgc gcgatggtga cggtcaggtg cgatggggtt tgggcaccga tcccaacatg
      541 ctcaccgcat cgctgcacgc ggtggtgggc gccgtggtgc gccacacgaa ctgagccgct
      601 ccacggtggc cgacaacaat tgtcagccac actgaaagaa ggtcttgccg ggccaccggg
      661 tgtgggctag cttgaccggc gcagtcgcat cgtcgacgcg ttcgactccg aatgttggct
      721 gccgggcacg gcgcaatcgc agggccggga tgaagggacg cttcccggcc cgtgcgagcc
      781 gtgcccgttc gcgtctgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncggaccc
      841 acagccgcgc gaaaggggga cccgatgaag gttcatgtca acttcgacgt ctgcgcgtcg
      901 accgcgtcgt gcatgcagac cgctcccgag gtgttcgagg tccgcgccga cgggtacctc
      961 tatgtgttgc aggaagagcc cggcgaagag ctgcgcgagg ccgtcaccga cgccgccgac
     1021 ctgtgcccca ccggagccat caccatcgaa ggctgaccca gccgagtcgt aggcgcaagt
     1081 gtgttcactc cacccctcct gtgggagcca actgaacaca catgaagtca gcccgcccaa
     1141 gtgtgttcga tccacccctc ctgcgcgggc caactgaaca cacatccgnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncagcggg gtgacgaggg cgatcgtgcc
     1321 gagcgtcgcc agggcaacca ggccgggcag actcgtcagc gacgtgatgc tggcccacgc
     1381 gttggtcaag gtggcccggt gcgcgtcggc agcggtccgc aactcgttca actccttacc
     1441 gagcttgatc agcggcgtcc gttcttcatg gtcgccgtcg cgctcgacca ggttgtagac
     1501 cgattcgcgc agcctcgggt cgttcaaggc ggcctggagt tcgaccgcgg tcgcaggcag
     1561 ggtggccgag acgcgccgca ctcgacggat ggcgagccag acgatcaacc ccggaacagc
     1621 acagatcacc agcccgacga tgccccacgc ggcgtgccac gacgatggga aggcgaacca
     1681 cccgaccgcg taggcgagca cgccgccagc caaggcgaaa agcagcgctc ccctggccag
     1741 caccctggct cggcgggcga cgacgccgag gcgatcagcg gccgattggg cgatgacgac
     1801 aacatcaaag cgggcagagg tcatgcctca gtctgtgcgg tcacgacacc ccggtcaatc
     1861 gaatcgcaac catctcccgc tgccaacaga tagatcgtaa tgcgatgtag ctgtcggtag
     1921 gctgcgccac gtgctgacgt cgctgtccag gtctgcccgt gttcgcaact ggaccgcagg
     1981 tctccagcgg ctcaccgact cgctcggcgt gcagcgccgg atggcagttg cgatcggcct
     2041 cagcggcctc accggcggcg tcatcggggc ggcgtatctc accgtgctcg atgtgaccaa
     2101 ccgtgcgatc ggccccggcc gctggtcgcc cggaccacac ctggtgatct tggcactggt
     2161 cggcgtgacc gttgctgctc tggtgaagtg gctcgggccg ccggccaacg tcgagttgct
     2221 ggtcgacaac atccacgtac cgggcatgtc gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnncgctgc tgtgcaccgg tgccgggggc acgctcggac cggaggcacc
     2341 gctggtcacc accaccggta cgatcgctca ccgccaggcg tcgcggatcg gcctcgatcg
     2401 gggtgacatc agggtcgtgt cgatcgcggg catgtcggcc gggttcgccg tgctgttcgg
     2461 ggcaccgttg ggcggggcga tctttgccct ggagatccct catcgccgcg gcatcggcta
     2521 tcaggaagca gcgatccccg cttgtttcgg agcgatcatc ggccacgcct gttcgaccgc
     2581 cgcgggccac atgggcctgg aaccgatctg ggactttccg atgatcaacc acctcaagtg
     2641 gctcgacctg ccgtggtcgt tgctggccgg cgtcatcggt gccgccatcg ctgtggtgtt
     2701 caccgtcctg gtccgtgcca tggcgaaggt gatgcgtacc acgccgctgc ccagccgccc
     2761 ggtgatcggc gggctcgtcc ttggtggcat cgctctggtc tcgccgtatg tgctgaccaa
     2821 cggcgaacta cagatcgacc acctcgccaa gacggccggc gtcgccgcac tgctcgttgc
     2881 agccggggtc aagctggtcg gcgccgcgac ctgcgtcgcc accggttggc aaggtgggtt
     2941 catcatcccg ctgttcttcg ccgggttctg cgtgggcttc gcgctggcgc cgcatctgcc
     3001 gggcgcttcg gtctggccgt tcgtgatcgc ggtgatgatc tccgccaacg tcggcgtcac
     3061 caagacccca ctcgggtcgg ccctcgtcgt caccgagatg gccggagcgg tcatgttgcc
     3121 gaccgccctg atcgctgcgc tcacctcgct ggtgctgacg tcgcaggtcc atctgatcga
     3181 gtcccagcgc gaccgtgtcg acgtctacgg tcggcgtcat gacgagacgt gaacgggcgt
     3241 tgaacaacgc cgagtaccgg tcgctcgccc agttccgcta cgccctgcgc cagttcctcg
     3301 ccttctcggc agatgccgcg cgggccgagg gactgacctc cgctcagcac caactgctgc
     3361 tgaccgtgcg tggtgcagag gccgacaagc gccagccgtc gatgagcgac gtcgccgagc
     3421 acctccagct caagctgcac tcgaccgggg agttggtcgc tcgcgccgtg gaagccgggt
     3481 tggtcgagcg cgtcaccgac ccggccgacg cgcgccgcgt gttgattgcc accaccgccg
     3541 cgggacgagg ccatctggag cgcctgtcgg tgctgcaccg cgccgagctc cgccgcttcc
     3601 gcaccgagat gaacgccctc ctcagagcca tcgacacccc cacctgacca cgtgtgttca
     3661 ctccaccccc cttgccgggg tcaatcgaac accgtccccc gtcgccaccg gtagtgcgtt
     3721 cagtccaccc ctcgcaccgg ggtcaatcga acaccatccc ccgtcgccac cggtagtgcg
     3781 ttcagtccac ccctcgcacc ggggtcaatc gaacaccgtc gcgacgcgcg gtgcggatgg
     3841 cgtcgcgggt cttgcgggcc tcggtggctg cggccgcagc gaagtcggtg ccttggctgg
     3901 cgtagaggat cgctcgggac gagctgatca ataaccctcg accagaggtt gaggctcctg
     3961 ccgcaacggt ggcagcaatg tcgccgccct gggcaccgat gccggggacc aagatcggca
     4021 ggtcaccgac gatgtcgcgg accgtcgcca actcgccggg gtaggtgcca ccgaccacca
     4081 gcccacactc accgatctgg ctccattgcc gcgcgacacg ctcggccaca tggcagtaga
     4141 gagggcgacc gtcgagcacg agcgactgga agtcgtctcc acccgcattg ctggtgcggc
     4201 agagcaagat cacaccgcgc ccggcgtggg cgaggaacgg ttcgactgag tcggtgccga
     4261 ggtacgggtt gacggtcaca gcgtgggcgc cgtagcgatc gaacgcctcg cgggcgtact
     4321 gcgcggcggt ggggccgata tagggatcga agatcgagat gagcgcggcc atcgccacgt
     4381 acgcgccgag cgcttccaat actgcttcag cccggttggc ggcgaagtag gcgatctgcg
     4441 gcttgaacgc gcacgcaaag ggcgccgtgg cgtcgatgat ctcgcggcag aacgtctcga
     4501 tgccgtctgg ccgggtggcc aggtggggcg gcatcagcgc tggatcaggg tcgatcccaa
     4561 cgcacagcat cgagtcctgc gtagcccacg cggcatcgag tcggcgcaga aagctcaccc
     4621 cgccagccta cgaccacccc gccaccgccg tcgccgctca cccacagcag gcaactgtgc
     4681 tcacttcacc ctcgcagaag gggtggatcg aacacacttg ggcgggctga cccgatgtgt
     4741 gttcagttgg ccctgacagg aggggtggat cgaacacact tggnnnnnnn nnnnnnnnnn
     4801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnggcactc agggacagcg tggatatcag
     5041 acttgatccg attctcgccg ctcagctcgg cctcgtcacc cgtacccaag gctgggccgc
     5101 tggcgccacc ggcaagcagt tcgaccgact ggtcgcgtcg aacagactcg tcgtcgtcca
     5161 tcgcggggtg taccgcgatc ccgcgacgcc aacatcgttg gaacaacgcg cccttgcagc
     5221 cgtactcgcc tgcggcgctg gggccgtggc ctcgcaccga ctcggtctcg cgctgatcgg
     5281 catgcctcgg taccagagca atctggtcga ggtgacgagc ggtgggctgc accgaattcc
     5341 cggtgtggtc acccaccgca gcctgcgtcc tctaccacac acgaccgtgc gcggcgtccc
     5401 gacgacggca ccagcgcgga ccttgatcga cgtcggttgc ctggtcagcc aggcgctcct
     5461 cgcacggtgg accaccacct gggtaagcga acgggtcgtg acggtcacgg agttggagtc
     5521 cgagatggcc gccgcccgcg ggcatcgagg tgtgccactg ttgcgggccg tgctcgatga
     5581 cctcgaccgt cgccacgagg tcgccgattc tccggtcgaa gcgaagttgg gcatgctgct
     5641 ggaggacaac ggctttccgc cgctgacgct gcaccacgtc gtcacggtga gttcgggagc
     5701 cgagttcgag ttggattgga gctacccgca gtggcagatc ggcttcgagg tcgacggcta
     5761 tggcgtccac ctccgctcac acgagagctt tgaccatgac cgctttcgcc gtaacgagtt
     5821 ggtgatcgac ggctggacga ttctcaactt cagcgggcgc caggtggcac gacggtcgcg
     5881 gaccgtgctc gatcaagcca gacgagccct caccgaacgc agcctgttgc cgtaacagtg
     5941 atcgattggg cccggcggcg ggggtgaaac gaacacacat gagccggacc aatggaactg
     6001 tgttcgattg gcccctgctc tgggggtgga tcgaacacgg ttgggggcgt gctcagccgt
     6061 gcagggtggc gacggcgatg atgccggggg cggcgcgtag cgactcgacc gtgctggtcg
     6121 gaacctcggc cgtgggggcg atgagcatga ccgccgtgcc gggctccttg gcctggccga
     6181 cgtccatgtc ggcgatgttg atcccggcgt tgccgagcaa cgtgcccacc acgccgatca
     6241 caccaggacg gtcgtcgttg gtgaccatca gcatgtggtc ggcaggcgga atgtcgaact
     6301 ggtggtcgtc gatgctgacc agccgcggct cgccacggcg gcccgacaag gtggccgaca
     6361 tgctgtgccc agccccggcg acggtgatga ggttgacgta gtcggtggtc gcggtggagc
     6421 tgacctcgcg cacctcgacg ccgttgctct tggccaactg cggcgcgttg acgtaggtga
     6481 ccggctcggc gctgcgtgcc gcgtagaaac ccttgagcac cgccagggtg aggatgcgcg
     6541 tgtcgtaccc ggcgagctcg ccttcgctgc gcacctcgag cgtggacggc gacgcgccga
     6601 ccagcgaggc gaacaggctg ccgacccgct cggccacggg caggaacggc ttgatcgtct
     6661 cgttggcctc ggccgcgttg acgttgaccg cgaacggcac gaagtcgccc gcgagggcca
     6721 actgcaccat gtcggcaatc gtgtcgccgg ccttgtcctg cgcctcgcgc gtgctggcgc
     6781 caaggtgcgg tgtgacgacc accgagtcga gcccgaacaa cggcgactcg gtgaccggct
     6841 ccttgatgaa cacgtcgagc gcagcgccgg cgacgacgcc atcacgaatc gcatcggcaa
     6901 gggcatcctc gtcgacgatg ccaccgcgag ccacgttgat cacccgcagc gacggcttgg
     6961 ccnnnnnnnn nngagcagca gatcgcggcc gatgatgccg gtcgtgtcct tggtcttggg
     7021 cagatggatg gtgaggaagt cactctcggc cacgagttgg tcgaggtcca tcatctcgat
     7081 gcccatcgtc cgcgcccggt cgggggcgac gtagggatcg aacgccacca ggcgcatgcc
     7141 gaagcccttg gcccgatcgg cgaccagctt gccgatgcgg ccgagaccga cgatgcccag
     7201 cgtcttatcg gccagctcga caccttccca cttcgaccgc tcccaccgcc cggccttgag
     7261 cgcggcgtga gcctggggca cgttgcgggc ttggctgagc agcagcgcca tggtgtgctc
     7321 ggcggcggac acgatgttgg actgcggcgc gttgaccacc atcacacccc ggcgcgtggc
     7381 ggcttcgacg tcgacgttgt cgagaccgat acccgctcgg ccgaccacca tcagctcgtc
     7441 ggccagttcc aacagcgcgg cgtcgacttg ggtggcggag cgaatgatca aggcatgggc
     7501 acccttgatg gcactgggca gcttggtgag gtcgagttcg acgtcgacca catggccggc
     7561 gccacgcagt cgatcgaggc cgccttcggc gatctcttcg gtcacgagga tgcgagacat
     7621 gaccaccatc cttaccagcc cagtcgggat tcacccagcg gattgaacga ttcgtcaacg
     7681 gccatctgac acatcggacc tagtgtgcgg gacgtggctg agcagggcgt gacgggggcg
     7741 cacgacctcg atcggatcga gcgctggttc atcgaccgcg gggtgcccca cttcatcgag
     7801 tcgtacgagg cgcgtagcga catctggaca cgagcgatcc cgatcctgct cgtcgcctac
     7861 ttcgtcggcg gcttccaggc gctcgacatc cgcggctgga gcctggccaa gaacctgggt
     7921 gcggccgcga tcgtggtggc ggtgctgatg ctgagctgnn nnnnnnnnnt cgcgctacga
     7981 cggcgctggt cgagcatgcc gcgcgagatc ggtcatgtcg aactggccgc gttcgtgatc
     8041 ggtccgtcga tcccgtcggc gttgttcggc caatggggcg acgcgctgca agccgcactg
     8101 atcggcgtgg ccgtgctggc ggtgatctac ctcggcacca gctacgcggt gataccgatg
     8161 atgcgctggg cgatgcaccg cgctgcgggt caggtgcgct cgatcgccaa cctgatcgtg
     8221 cgcgccctgc cgctgctgct gttgttcgcc acctttctgt tcatcaacgc cgaggtatgg
     8281 caggtggccg gcgcgctcga cggccccgcc tactggctga cgttggcgat cttcttcgcg
     8341 ctcggcgcgc tgttcgcaat ctcgcgggtg ccggggctga tgcaggacct ggctcggttc
     8401 gggacgtgga ccgacgtcgc tgcactggtc gacggttccc ccgccgctgg cctggacacc
     8461 acccctgccg acggctgccc cgagccgacc acactcgggc ggcgccagcg gttcaacatc
     8521 gcgctgctga tgctgttcgg tcagggccta cagatcacgc tggtggcgct gatgctgacc
     8581 gggttcttcg tgctgctcgg gttcctggcg atcagcgaac agacagcgct gtcgtggacc
     8641 acgctcagcg acgtccacgt gtttgcccgg ctgcacctcg gccaacgaga gctggtgctg
     8701 agcgggccgt tgctgcgcgt cgccgggttt ctcggcacgt tcagtggcat gtacttcacc
     8761 gtggtgtcgg ccaccgacgc cacctaccgg gacgagttcg ccgaagacgt ccgccccgag
     8821 gtccagcgca tcttcgccgt ccacgccgtg taccgctgcg ccctgcgcgc caaggcggcc
     8881 gcgacggagc cgttccggta ggttgcacgg ccatggcatt ccagttcctg tccgacgcat
     8941 ggatggtcga agcgcgggcg atccgcgcca agtacgaggg tgaggtcccg cccgtcaccc
     9001 agtcgatccg gatcaacctc gtcatcaccg acgcgccgtt tggggacgac gagacggcga
     9061 gccaggtcga cgccttcatc gacaccagcg gcggctcgat ggtgatggac ctcggcacgc
     9121 tcgatcagcc cgacaccacg atcaccaccg actaccccac tgccaaggcc ctgttcgtcg
     9181 accaagacgc ggccgcggca atgcaggcat tcatggccgg caagatcagg gtgcagggtg
     9241 acatgaccaa gatgatggcc ctcagcacgg cagtccccgc gtcggaagcc acgcagcaga
     9301 tcgccgagga gatcaaggcg atcaccgccg actgagcgcc cggcggcgcg acctgcagag
     9361 cagcgtcagt cgcgcaccag cggggcgagc gcgtcggcca gttcggacac cgtccagcgg
     9421 ctgtcacgct cgacggtgtc gcccatggcc cagctgtcga cgcgggtgac ccggccgcct
     9481 tgcacgaaga aggtctcgcc ggtgaaggca cactcggcgg tcgccaggta ggcgaccagc
     9541 ggggagatgt tggccgggtc ccacatgtcg aacgcgccgt cgcgcgggtt catgatgtct
     9601 tccagccccg gtgtggccag ggtgaggcgg gtacgtgcgc cgggtgagat gcaattgctc
     9661 ttcacgccgt agcgcaccag ttccttggcg cagatctggc tgaaggtggc gatcccggtc
     9721 ttggccgcgc cgtagttggc ctggccaggg ttggcgaaca gaccggacgt ggaggaggtg
     9781 tggacgatgt tgcgcggctt gtcgatgccc gccttggtct gctcgcgcca gtacgcggcg
     9841 gcccagcgag tcggcgcgaa gtgacccttg aggtggacgg cgatcaccgc gtcccactcc
     9901 tcctcggtca tgttgaccaa cacccggtcg cgcaggatgc ccgcgttgtt gacgaggatg
     9961 tcgagatcac cgaaggcctc gatcgcgctg ttgatcagcc gctgcgcacc ctgccagtcg
    10021 gcgacgttgt cgccgttgac gatcgcctca ccgccgagtg cttcgatctc ggccaccacc
    10081 tgttgagccg gagtgagatc ggtgccggtg ccgtcgttgg caccgccaag gtcgttgacc
    10141 accaccttgg ctccttcact ggcgaacagc agcgcgtgtt cacggccgat gccgcgcccc
    10201 gctccggtga tgatggcgac acgaccttcg agagaaccca tgccggggaa cgctaaccac
    10261 cacgctgcca gcgcaacgaa ccacgacgta gcgagttggc cagcagctgt cagccggaca
    10321 acggtcagtc acaaccgtga gcaaccggtg aaccggtcaa ccggtcagcc gtcggtcggc
    10381 ggcgcgtcgg tggacggggg cggaggaggc ggcgggtcgt tgtaggcgat gtcgatcacg
    10441 ctgcccttga tcagcggttg accgctcgcc accggttggc caccgctggt gaccgactgc
    10501 agtgtgcccg agccgtcggt cggtccggtg accgcgccga cgaccaaccc aaccacggcg
    10561 atcgcgtcga ccaactgttg gtgggtctga ccctcgatgg tcgggatcgc cacaatctcc
    10621 ggccccttcg acaacgcgaa cgagaccgtg tcgcccttgt tgagcggctg gtccatgggg
    10681 aactgctgcc aggcgacctg cccggctggg acgtcgaggc tgaagatgtc gtccgacttc
    10741 gcaccgacca gaccgagagc ggccaacagt tgcgtggcgg tcacctcgtc gagcatgccc
    10801 agttcgggca tcaaccgctg cgccggcccc ttcgacacca ccaccgcgac gaccgaaccg
    10861 ggcatcacct cggccccggc ggtggtgttc ggctgagcct cgtaacgcca cgagatcacc
    10921 gacccggccg gcacgtcggg gcacgtgatc gacgccgcag ccagcccgct ggccggcggc
    10981 gcgctctcgc agaactgctc gctgtcgacc cgcatctgca gcttggccgc ggtgagggtc
    11041 tgttcggcca ccgccagttc catgccggtg acgtcgggca gggtcgacag gttggggcct
    11101 tcgctgacca cgaacaggat cgtgccgcct tcggccaacg tgaccccggc gaccgnnnnn
    11161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnggcc accagcagcg
    11341 acacgatcgt cagcacccag aaccggccgc ggccacggcg ccgaggcgcc agatcgatgt
    11401 cgtccgtctg gtcgtacagg cgtgccgtac cacccgtgtg gtcgagcgct cgcggtgcga
    11461 tgcgcatgcc gccctcggcc ggagccggag caggcgcggg tgccggaggc acctcgacga
    11521 ccggcggcgt cttgacgcgc ggtgccgacg aggtcgggcg aggttcggtg gccatcgaca
    11581 ccgagaacga cccg
//
LOCUS       DAORNF010000011        11543 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_4221_length_11543_cov_1.680884, whole genome shotgun sequence.
ACCESSION   DAORNF010000011 DAORNF010000000
VERSION     DAORNF010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 11543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11543
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4221_length_11543_cov_1.680884"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(208..1464)
                     /locus_tag="PLV68_00950"
     CDS             complement(208..1464)
                     /locus_tag="PLV68_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRD99831.1"
                     /translation="MTDLDLSASPRSIALRERVEAFMDEHVYPNEERFFRESVELGPW
                     AVWPVVDELKALAHEAGLWNLFLPDSNEGAGLTNLEYASLCEVMGRSMLAPEVFNCNA
                     PDTGNMETLVRYGSPEHHERWLKPLLAGEIRSAFAMTEPAVASSDATNIESSIVRDGD
                     EYVVNGHKWYTTGATHPNCNIIIFMGKSDPANPDRHSQQSMVLIPRDTPGVEVLRPIP
                     VFNFYGMPDRASEVRFTDVRVPVSNILLGEGRGFEIAQGRLGPGRIHHCMRLIGLAER
                     ALELMCRRTQHREAFGGPISGHGVTLERIAEARIAIEQTRLLTLKAARMMDTVGNKAA
                     RQEIAMIKIAAPNMACKVVDWAIQAFGGAGTGNDFGLAAMYALARMLRLADGPDEVHR
                     NQLARLELKRHAALGPVQPRPYAPTW"
     gene            1501..1668
                     /locus_tag="PLV68_00955"
     CDS             1501..1668
                     /locus_tag="PLV68_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99832.1"
                     /translation="MGGSPDMTYQLKSQDITPPPVDPARQRSVAKDGAAAVAMIVITA
                     GLITLILSQVI"
     gene            1689..>2365
                     /locus_tag="PLV68_00960"
     CDS             1689..>2365
                     /locus_tag="PLV68_00960"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRD99833.1"
                     /translation="MTSILHEQPLLPHLVVTALESRSNEPCIFTGAGASGDAPGAASV
                     LTYGQVRDRTSQMVQAMRSLGVGRGAHLAILSKNRPEVLLNACATLVNGCILTPLHPM
                     ASLGDHSYAVRDAEIDCLVFDAAHFTERAAELRDQYPDLVLLGFGPNAAGHDYLALAD
                     TFSPQPLVAPDVHLDDLCTIVYTGGTTGRPKGVLMTHRVWQSMTWIQMAEWEFPTEIR
                     IALPTPLS"
     assembly_gap    2366..2378
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2379..>2722
                     /locus_tag="PLV68_00965"
     CDS             <2379..>2722
                     /locus_tag="PLV68_00965"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRD99834.1"
                     /translation="VVAPVLISGGTFYVMESFSPDSFFDLVEQHRITCAMIVPVMLYA
                     MQASPRYRTADMSSMETLIYGASPISPAKLAEAIEQWGPIFFQFFGQTEAPMVITHLK
                     KADHDLAHPERL"
     assembly_gap    2723..2896
                     /estimated_length=174
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2897..>3201
                     /locus_tag="PLV68_00970"
     CDS             <2897..>3201
                     /locus_tag="PLV68_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRD99835.1"
                     /translation="LHTGDVGRFDDEGFLYIVDRTKDMVITGGFNVFPREVEDVISQH
                     SAVSMVMVIGVPDPKWGEAVKAVVVLAPGAAASDELTVELQTMVKNAKGSQQAPKSI"
     assembly_gap    3202..3405
                     /estimated_length=204
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3406..4687)
                     /locus_tag="PLV68_00975"
     CDS             complement(<3406..4687)
                     /locus_tag="PLV68_00975"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRD99836.1"
                     /translation="MESAVIFDPHGSGSIARRRGRVRYALALVATFGLLAAACGSDEK
                     SSSSSSTTTTAASSTTGGPAADGTPIKVMTVTTLNANGPTYENIAITAKLFADDINAR
                     GGINGHPLQVTVCDEQFDPAVAATCARQAAEEGMVSVVGSFTYFAESIVPVIAESDIT
                     WFGACCPISPSELSDPHSFNLGNQPMYAVGAVKRAVEDGCENINAAIAEGADAIFKPP
                     MENAMKAYGRSFGQIVIVPAVAQDYSAEVARATSGADCVVSVMSETPFITWNTAWAQS
                     GTTAQQYGNQGNLNEISAKGAETATNGNIIAGMYPDISTKPWDAYRAALDKAGIDQVK
                     SDFNSLGGMGTWAAYTAFAQIAATIQGDITAKSFYEAASATTDLDLGGMVPPIDFTKE
                     WTDGLPGYNRLFNRTVVYSKLENGKVVPVDNNFVD"
     gene            4885..>5185
                     /locus_tag="PLV68_00980"
     CDS             4885..>5185
                     /locus_tag="PLV68_00980"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99837.1"
                     /translation="MNEVVRFALLGLGVGALYALASQGLIVIFRGTGVLNFSLGATAI
                     AGVFLQWELQTRAGWPFLLSAVFGVALSAVLGGLTHWVILRPLQRKRASTLVQLLA"
     assembly_gap    5186..5374
                     /estimated_length=189
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5435..>6011
                     /locus_tag="PLV68_00985"
     CDS             5435..>6011
                     /locus_tag="PLV68_00985"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99838.1"
                     /translation="MGVSPNKVAMLNWALGSSVACIAGILVVPIITLQVTAMTSLVLA
                     ALAAALVADFKSFPIATAAGYLLGMGQTLVGNYWTQQGVGQSLPFIVIIVVMVVRGRA
                     IPMRDHFLQKLPTVGSGRMSWDWTLFLTGAVVFLMLTKDTKWIDALTVSLCAAIILLS
                     IVVLTGYAGQLSLAQYAIAGFGAYVGGRLVAV"
     assembly_gap    6012..6038
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6039..7655
                     /locus_tag="PLV68_00990"
     CDS             <6039..7655
                     /locus_tag="PLV68_00990"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HRD99839.1"
                     /translation="PFLAGLGLGVLGAIPLGLIFALPAVRTRGINLAVVTFGLGSAIE
                     LILFRNRNLTGGIKGTPVGHPSLFGYDISSIRHPQRYGIFVLAMAVLAVWVVANVRRG
                     RSGRRLIAVRTNERAAASLGINVVKLFAFAFASAIAALGGVLLAFRVTSISYQSFTSF
                     TSIYFAGIALVGGVGHLLGAFVGATMATAGFNQEVMESTWEGVGKYVQLISGIAILLT
                     VLANPDGVVASWVKSFRVMKRTKKFGESYFIKLSDVSEPADESHSQRVAPKELEVSGL
                     TVRYGAVTAVGDVSFTLRPGTVTGLIGPNGAGKTSLIDALTGFTRSSGRVSIGGQEIS
                     GAGAAARVKSGLARSFQSLELFEDTTVFENLSVAADPQDFRSYVRDLVWPVIPKFGPD
                     VVRAIEEFELDEDLHREVSDLSYGKRRLLAIARAVAMHPSVLLLDEPAAGLSSVESVE
                     LARVVRRLADDWGIAILVVEHDMNFVMGVCDEVIVLDFGVLIASGPPDKVRNDPGVIA
                     AYLGDNTDDDLDASAAAIRANAVAVAGGES"
     gene            7655..>7853
                     /locus_tag="PLV68_00995"
     CDS             7655..>7853
                     /locus_tag="PLV68_00995"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRD99840.1"
                     /translation="MSATTDTSPYAISHTPNLLETRALKAGYLGREVVTGVNITVRPG
                     EVVGLLGPNGAGKTTTLMTVAG"
     assembly_gap    7854..7863
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7864..>8203
                     /locus_tag="PLV68_01000"
     CDS             <7864..>8203
                     /locus_tag="PLV68_01000"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRD99841.1"
                     /translation="VMFSNVPTFTPMYQRVRAGIALVTEERLVFTKMSTRDNLRIGGS
                     DLEQALALFPELHERLDVRAGMLSGGEQQMVALARALSRDPQLLLADELSLGLAPMIV
                     DRLLAAVRAAA"
     assembly_gap    8204..8241
                     /estimated_length=38
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8242..8577
                     /locus_tag="PLV68_01005"
     CDS             <8242..8577
                     /locus_tag="PLV68_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99842.1"
                     /translation="RKALKYSDRMYLMARGRILLELPSAEALQRLDEIEEAYLSGTSE
                     TEEDHIERKRRKDARKAWGRQRVRERELRRLSGQQVSTRRTSEDDHYLDNDQMRREWQ
                     QRLRRGRKY"
     assembly_gap    8725..8734
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8735..>9595
                     /locus_tag="PLV68_01010"
     CDS             <8735..>9595
                     /locus_tag="PLV68_01010"
                     /inference="COORDINATES: protein motif:HMM:NF023500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3556 domain-containing protein"
                     /protein_id="HRD99843.1"
                     /translation="FYVWKIFFYSLFGLIVAGAFTRGLEFNDIAGWWDQPILYQKLMI
                     WTILMEILGLAATCGPMAFHFDPPIGGVLYWWQNDTIRIPPYPNHVPFTAGNRRTPWD
                     TGLYKLIVFWLVMMLFLSGDQIPGLPEGSAGVLPRWALITYAGLIVLMGLRDKIVFLS
                     SRAEQYVPTLLCFGLFANFVDMIVAAKIFIVVVWMGAGISKLQHGFSATVAVMMQNTP
                     WNGFRKLRMATVRNYPEDIRPSKFTHFIAHFLGTTTEIVMPLILLFSPWPWLTWIAIV
                     SMWLLHAFIIS"
     assembly_gap    9596..9697
                     /estimated_length=102
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9698..10382
                     /locus_tag="PLV68_01015"
     CDS             <9698..10382
                     /locus_tag="PLV68_01015"
                     /inference="COORDINATES: protein motif:HMM:NF023500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3556 domain-containing protein"
                     /protein_id="HRD99844.1"
                     /translation="ALLIAVIAFALFPIVLGAIWPKYVSFLIGLKQYAGNWAATTWSF
                     RDKEKEDRLNERIVKAADNQIDQIEPLFGKEISEIFIQKAIAFRMMHPMGRMHITAHM
                     RHSGDIDTRVLREGEFVSNVLTVLNFGDGHCHDERLIDAVQQRCNYEPGDLVVCFTES
                     QPMFSKKVQYRVIDAAVGVVEKGWYHNDDAANEQPWLPNGLLPHTVTWTRDGYVAPGA
                     PYPGNGGRS"
     gene            10401..>10771
                     /locus_tag="PLV68_01020"
     CDS             10401..>10771
                     /locus_tag="PLV68_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRD99845.1"
                     /translation="MTRAVVVGSGPNGLAAAVALARAGIEVTVFERADTIGGGTRSSD
                     AIVPGLIHDHCSSAHPMAIASPFFQQMQLPGITWRAAEIDCAHPLDDGSAGAFARSFD
                     VTADALGADGRRWRRMFEPIAR"
     assembly_gap    10772..10781
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            10862..>11543
                     /locus_tag="PLV68_01025"
     CDS             10862..>11543
                     /locus_tag="PLV68_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99846.1"
                     /translation="MPRHPLTLARFGMRALPPATVLARALSTPQARALWTGVAAHAFW
                     PLGSPLTSAVGVALTAGAHAHGWVVAEGGSQAIADALAADVVAHGGRIETGVEVTSVE
                     QLGGPDVVMLDVSPSVALAILGDLLPSRVARAYRRFRHGPGAFKVDFAIDGDVPWVAE
                     VARRAGTVHLGGPMAEIVAAERDLHRGRMPERPFVLVGQHGQRPGGRGGLLSIREVGQ
                     VGVIGGQSI"
BASE COUNT         1677 a         3471 c         3611 g         2007 t
ORIGIN      
        1 acctgctggc gatgaccccg tactggtgga gtgccggtga agcgctgcaa caagcgatac
       61 ccgccgtgca ggtcaccgcc cacttcaccc tccaaaccct caccgcccca accgagtgat
      121 gcgaaatcgt ccgccgcgct gtagcgctcg gcggcgagtc atcgcgcgtc ggcgtgaacc
      181 cgttgcccga ctaccaggtg ggcgcgatca ccaggtgggg gcgtaggggc ggggttggac
      241 ggggccgagg gctgcgtggc gcttgagctc caggcgggcg agttggttgc gatggacctc
      301 gtcggggccg tcggccaggc gcagcatgcg ggccagggcg tacatcgcgg ccagtccgaa
      361 atcgttgccg gtgcctgcgc cgccgaaggc ctggatcgcc cagtcgacca ctttgcacgc
      421 catgttcggc gcggcgatct tgatcatcgc gatctcctga cgggccgcct tgttgccgac
      481 ggtgtccatc atgcgcgcgg ccttcagcgt gagcagccgg gtctgttcga tggcgatgcg
      541 tgcctcggcg atgcgctcga gcgtgacccc gtgcccggag atcggcccgc caaaggcctc
      601 gcggtgctgc gtgcgacggc acatcaattc cagtgcccgc tcggccaaac cgatcaggcg
      661 catgcagtgg tggatgcggc ccggcccgag gcggccctga gcgatctcga acccgcggcc
      721 ctcaccgagc aggatgttgg agacgggcac gcgcacgtcg gtgaatcgca cctcggaagc
      781 ccggtcgggc atgccgtaga agttgaacac ggggatcggc cgcagcacct cgacgccggg
      841 tgtgtcacgg gggatcaaca ccatcgactg ttggctgtgc cggtcggggt tggccgggtc
      901 ggacttgccc atgaagatga tgatgttgca gttcgggtgc gttgcgccgg tggtgtacca
      961 cttgtggccg ttgacgacgt actcgtcgcc gtcgcgcacg atcgagctct cgatgttggt
     1021 cgcgtcgctg gacgcgacgg cgggctcggt catggcgaag gccgaccgga tctcacccgc
     1081 gagcagcggc ttgagccagc gttcgtggtg ctcgggtgag ccgtagcgga ccagggtctc
     1141 catgttgccg gtgtcgggcg cgttgcagtt gaacacctca ggagccagca tcgagcggcc
     1201 catcacttcg cacagcgagg cgtattccag gttggtcaac ccggcaccct cgttgctgtc
     1261 gggcaggaac aggttccaca gacctgcctc gtgcgccagc gccttgagct cgtccaccac
     1321 cggccatacg gcccacggtc cgagctcgac cgactcgcgg aagaagcgct cctcgttggg
     1381 gtagacgtgc tcgtccatga aggcttcgac ccgctcccgc agagcgatcg aacgaggtga
     1441 agcgctgaga tccaggtcgg tcatgggctc acggtacgct acgccgcgag acggtccgaa
     1501 atggggggtt cgccggacat gacctaccaa ctcaagagtc aggacatcac gccgccaccg
     1561 gtcgacccgg cacggcagcg ctcggtcgcc aaggacggtg ccgctgcggt cgcgatgatc
     1621 gtcatcaccg ccggactgat cacgctgatc ctcagccagg tcatctgagc tggtcgggag
     1681 cgttggcgat gacgtcgatc ctgcacgagc aacccctgtt gccgcatctg gtcgtcacgg
     1741 ctctcgaaag ccggtcgaac gagccgtgca tcttcaccgg cgccggtgcg agcggtgatg
     1801 cgccgggtgc cgcgagcgtg ttgacctacg gccaggtgcg cgaccgcacc agccagatgg
     1861 tgcaagcgat gcgctcgctc ggtgtgggcc gcggcgctca cctggcgatc ctgtccaaga
     1921 accgccccga ggtgttgctc aacgcctgtg ccaccctggt caacggatgc atcctcacgc
     1981 cgttgcaccc gatggcgtcg ttgggtgatc actcgtacgc ggtgcgcgac gccgagatcg
     2041 actgtctggt gttcgacgca gcgcacttca ccgagcgcgc ggccgagttg cgcgaccagt
     2101 atcccgatct cgtcctgctc ggcttcgggc cgaacgccgc gggccacgac tacctcgcgc
     2161 tggccgacac gttctcgccc cagccactgg tcgcgcccga cgtccacctc gacgatctgt
     2221 gcaccatcgt ctacaccggt ggcaccaccg ggcgtcccaa aggtgtgctg atgacccacc
     2281 gcgtctggca gtcgatgacg tggattcaga tggcagagtg ggagtttccc accgagatcc
     2341 gcatcgccct gccgaccccg ctctcnnnnn nnnnnnnncg gtggtcgctc cggtgctgat
     2401 cagcggcggc acgttctatg tgatggagtc gttcagcccc gactcgttct tcgatctggt
     2461 cgaacagcat cgcatcacct gcgccatgat cgtgcccgtc atgctctacg cgatgcaggc
     2521 cagcccccgc tatcgcaccg ccgacatgtc gtcgatggag acgctcatct acggagcctc
     2581 gccgatcagc ccggccaagc tggccgaagc gatcgagcag tgggggccga tcttcttcca
     2641 gttcttcggc cagaccgaag cgccgatggt gatcacccac ctcaagaagg ccgatcacga
     2701 tctggcccac cccgaacggc tcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnngttg cacaccggtg acgtcggccg cttcgacgac gaaggcttcc
     2941 tgtacatcgt cgatcgcacc aaggacatgg tgatcacggg cgggttcaac gtgtttccgc
     3001 gcgaggtcga agacgtgatc agccagcact ctgcggtgtc gatggtgatg gtgatcggcg
     3061 tgcccgaccc gaagtggggc gaggcggtca aggccgtcgt cgtgctggcg ccaggcgcgg
     3121 ctgcgtccga cgagctcacc gtcgaactgc agaccatggt caagaacgcg aaaggttccc
     3181 agcaggcgcc gaagtcgatc gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncgtcg acgaagttgt
     3421 tgtcgaccgg aacgaccttg ccgttttcga gcttgctgta cacgacagtg cggttgaaca
     3481 agcggttgta gccaggcaga ccgtcggtcc actccttcgt gaagtcgatc ggcggcacca
     3541 tcccacccag gtccagatcg gtggtcgcgc tggcggcctc gtagaacgac ttggctgtga
     3601 tatcgccctg gatcgtggcc gcgatctggg cgaaggccgt gtaggcggcc caggtgccca
     3661 tgccgcccaa gctgttgaag tcgctcttga cctgatcgat ccccgccttg tcgagcgcgg
     3721 cccggtaggc gtcccacggc ttggtcgaga tgtcggggta catgccggcg atgatgttgc
     3781 cgttggtcgc cgtctcggca cccttggccg agatctcgtt gaggttgccc tggttgccgt
     3841 actgctgggc ggtggtgccc gactgggccc aggccgtgtt ccacgtgatg aacggcgtct
     3901 cggacatgac cgacacgacg cagtcggcac cgctggtggc gcgggcgact tcggccgagt
     3961 agtcctgggc gacggcgggg acgatgacga tctggccgaa gctgcgaccg taggccttca
     4021 tcgcgttctc catcggcggc ttgaagattg cgtcggcacc ttcggcgatg gcggcgttga
     4081 tgttctcgca gccgtcctcg actgcccgct tgaccgcgcc gacggcgtac atcggctggt
     4141 tgccgaggtt gaacgagtgc gggtcgctca actccgacgg cgagatcggg cagcatgcgc
     4201 cgaaccaagt gatgtccgac tcggcgatga ccggcacgat cgactcggcg aaatacgtaa
     4261 acgagccgac gaccgacacc atgccttcct ccgcggcctg gcgggcgcag gttgccgcga
     4321 cggctgggtc gaactgctcg tcgcacaccg tgacttgcag tgggtgaccg ttgatgccgc
     4381 cacgagcgtt gatgtcgtcg gcgaacagct tggcggtgat cgcgatgttc tcgtaggtcg
     4441 gtccgttggc gttgagtgtg gtcaccgtca tgaccttgat gggcgtgccg tcggctgccg
     4501 ggccgccggt ggtgctgctg gccgcggtgg tggtggtcga cgaactggaa ctgctcttct
     4561 cgtcgctgcc gcacgccgcg gcgagcagac cgaaggtggc gacgagggcg agcgcgtagc
     4621 ggacgcgacc tcgccgtcgc gcgatcgaac ctgacccgtg tggatcgaag atgactgcgg
     4681 actccatgaa tatccccctc tagatcgcgg tgctgagcac gctgcggggg tgccggacgg
     4741 ctcccccgtc ggcgacttta ggcagcgctt cgcgttctgc gtagaacttt gccctcagtt
     4801 cgacgccgcg atacgttccc gaccggtgcc gcagcgcacg gtcgatggcg ggggccatcg
     4861 acggctcaga acaacagggg gaagatgaac gaagtcgtca gattcgctct tttgggcttg
     4921 ggtgttggcg cgctctacgc cctggcttcc caggggctca tcgtcatctt tcgtggcacc
     4981 ggggtgctca acttttcgct cggggccacc gcgatcgcag gcgtgttctt gcagtgggaa
     5041 ctccagaccc gggccgggtg gccgtttcta ctgtcggccg tgttcggcgt cgcgctctcg
     5101 gccgtgctgg gtgggctgac ccactgggtc atcctgcgtc cgctccagcg caagcgggcc
     5161 agcacgctgg tgcaactgct ggccannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntcgagc cagttcggcc tgtcgaccga
     5401 agcggtgtcc gagagcgaac gctcggcgtc cgcggtgggg gtttcgccga acaaggtagc
     5461 gatgctcaac tgggcgctcg gctcgtcggt cgcgtgcatc gcgggcatct tggtggtgcc
     5521 gatcatcacg ttgcaggtca ccgcgatgac cagcttggtc ttggcggcgt tggctgccgc
     5581 attggtcgcc gacttcaagt cgttccccat cgcgaccgcg gccgggtacc tgctcggtat
     5641 gggccagacc ctggtgggca attactggac ccagcagggc gtcggccagt cgttgccgtt
     5701 catcgtgatc atcgtggtga tggtggtgcg cggtcgagcg attccgatgc gcgatcactt
     5761 cctccagaag ctgcccaccg tcggcagcgg gcgtatgtcg tgggactgga ctctgttcct
     5821 cacgggtgcc gtcgtgttct tgatgttgac caaggacacc aagtggatcg acgcgctgac
     5881 ggtgtcgctg tgcgcggcga tcatcctgtt gtcgatcgtg gtgctcaccg gatacgcggg
     5941 ccaactgtcg ctcgcgcagt acgcgatcgc cgggttcggt gcgtatgtcg gcggtcgctt
     6001 ggtggccgtg tnnnnnnnnn nnnnnnnnnn nnnnnnnncc gttcctggcg gggttgggcc
     6061 tcggtgtcct cggcgcgatc ccgctgggcc tgatcttcgc cctgcccgcg gtgcggacac
     6121 gagggatcaa cctcgccgtc gtcacgttcg ggttgggcag cgcgatcgag ctgatcctgt
     6181 tccgcaaccg caacctgacg ggcggcatca aaggcacacc cgtcgggcac cccagtctct
     6241 tcggctacga catcagctcg atccgccatc cccagcgcta cggcatcttc gtgttggcga
     6301 tggcagtgct ggcggtgtgg gtggtggcca acgtgcgccg cggccgcagc ggtcggcggt
     6361 tgatcgcggt ccgcaccaac gagcgcgccg cggcctcgct cggcatcaac gtcgtcaagt
     6421 tgttcgcatt cgccttcgcg tcggccatcg ccgcgttggg cggggtgttg ctggcgttcc
     6481 gcgtcacctc gatcagctac cagtcgttca ccagcttcac gtcgatctac ttcgccggca
     6541 tcgcgttggt cggcggtgtc ggccacctgc tcggcgcgtt cgtcggtgcc accatggcca
     6601 ccgccgggtt caaccaggag gtgatggagt cgacctggga gggcgtcggc aagtacgtcc
     6661 aactgatcag cggcatcgcc atcctgctca ccgtgttggc caaccctgac ggggtggtcg
     6721 cgtcgtgggt caagtcgttc cgggtgatga agcgcaccaa gaagttcggc gagtcgtact
     6781 tcatcaagct gtccgacgtg tccgaaccgg ccgacgagag tcattcacaa cgggttgccc
     6841 ccaaggagtt ggaggtatcc ggcttgaccg tgcgttatgg cgcggtcacg gcggtcggcg
     6901 atgtcagctt cacgttgcgg cccggcaccg tcaccggtct gatcggcccc aacggtgcgg
     6961 gcaagacgtc gctgatcgat gccctcaccg ggttcacccg ctccagcggg cgggtcagca
     7021 tcggcggtca ggaaatctcc ggtgctggtg ccgcggcgcg cgtcaagtcc ggcttggccc
     7081 gctcgttcca atcgctggag ctgttcgagg acacgacggt gttcgagaac ctcagcgtgg
     7141 ccgccgaccc gcaggacttc cgctcgtacg tccgcgatct ggtctggccg gtgatcccca
     7201 agttcggccc tgacgtcgtg cgcgcgatcg aagagttcga actcgacgag gacctgcacc
     7261 gcgaggtcag cgacctgtcc tacggcaagc ggcgcctgct cgccatcgcc cgcgcggtcg
     7321 cgatgcatcc cagtgtgttg ctgctcgacg aaccggccgc gggtctgagc tcggtcgaaa
     7381 gtgtcgaact ggcgcgggtg gtgcggcgtc tcgccgacga ctggggcatc gcgatcctgg
     7441 tggtcgagca cgacatgaac ttcgtcatgg gggtgtgcga cgaggtgatc gtgctcgact
     7501 tcggagtgtt gatcgcgtct ggcccgccgg acaaggtgcg caacgacccc ggtgtcatcg
     7561 ctgcctatct cggcgacaac accgacgacg acctcgacgc cagcgccgct gccatccgcg
     7621 ccaacgcggt ggccgtcgca ggaggcgagt cgtgatgagc gccacgaccg acaccagccc
     7681 gtacgccatc tcccacacac cgaatctgct cgaaacccgg gcgttgaagg cgggttacct
     7741 cgggcgcgag gtggtgacag gggtcaacat cacggtgcga cctggcgagg tggtcggcct
     7801 gctcgggccc aacggcgcgg gcaagaccac caccctgatg accgtggccg gagnnnnnnn
     7861 nnncgtcatg ttcagcaacg tgcccacgtt cacgccgatg taccaacggg tgcgcgccgg
     7921 cattgcactc gtcaccgaag agcgtctggt gttcaccaag atgtcgacgc gcgacaacct
     7981 gcgcatcggt ggcagtgacc tcgagcaggc gctggcattg ttccccgagt tgcacgaacg
     8041 tctcgacgtg cgcgctggga tgctctcggg cggcgagcag cagatggtcg cgctggcccg
     8101 tgcgctcagc cgcgacccgc agttattgct cgccgacgag ttgtcgctcg gtctcgcacc
     8161 gatgatcgtc gaccggttgt tggcggcagt gcgcgccgcc gccnnnnnnn nnnnnnnnnn
     8221 nnnnnnnnnn nnnnnnnnnn ncgcaaggcg ttgaagtaca gcgaccgcat gtatctgatg
     8281 gcccgcggcc gcatcttgct ggagctgccc tcggccgagg ccctccagcg cttggacgag
     8341 atcgaagagg cctacctcag cggcacgtcc gaaaccgaag aagaccacat cgaacgcaag
     8401 cgccgcaaag acgcccgcaa ggcctggggt cgccagcgcg tccgcgagcg cgagctgcga
     8461 cgcttgtccg gccagcaggt ctcgacccgc cgcacgtccg aagacgacca ctacctcgac
     8521 aacgaccaga tgcgccgcga atggcaacag cgcctgcgcc gcggccgcaa gtactgacgc
     8581 cccactagga gagatcatgg gttaccgcac cgcagaactc ccacccgtcg acctcgacga
     8641 gttcgccacg atcccgttca tgaagcggat gaagttgctg caactgcact gggtcgaggc
     8701 tggtttcggc acgccgaaac agacnnnnnn nnnnttctac gtctggaaga tcttcttcta
     8761 cagcctgttc ggtctgatcg tggccggagc gttcacgcgc gggctcgagt tcaacgacat
     8821 cgctggttgg tgggatcaac cgatcctcta tcagaagctg atgatctgga cgatcctgat
     8881 ggagatcctc ggtcttgccg cgacctgtgg gccgatggcg ttccacttcg acccaccgat
     8941 cggcggcgtt ctgtactggt ggcagaacga cacgatccgc attcctccgt accccaacca
     9001 cgtgccgttc accgcgggca atcgccgcac tccgtgggac acagggctct acaagctgat
     9061 cgtgttctgg ctggtgatga tgctgtttct gtcgggcgat cagatccccg gtctccccga
     9121 gggttcagcc ggtgtcttgc cgcgctgggc gctgatcacc tacgccgggc tgatcgtgtt
     9181 gatgggtctg cgcgacaaga tcgtgttcct gtcgtcgcgc gccgagcagt acgtaccgac
     9241 cctgctgtgt ttcggcctgt tcgccaactt cgtcgacatg atcgttgccg ccaagatctt
     9301 catcgtcgtg gtgtggatgg gtgcaggcat ctccaagctg cagcacgggt tctcggccac
     9361 ggtcgcagtg atgatgcaga acacgccgtg gaacggattc cgcaagctgc ggatggccac
     9421 ggtgaggaac tatcccgaag acatccgtcc atcgaagttc acccatttca tcgcccactt
     9481 cctcggcacg accaccgaga tcgtgatgcc gttgatcctg ttgttctcgc cgtggccgtg
     9541 gttgacgtgg atcgcgatcg tgtcgatgtg gctgttgcac gcgttcatca tctccnnnnn
     9601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnggc gctgctgatc gccgtgatcg
     9721 cattcgcgtt gttcccgatc gtgctcggcg cgatctggcc aaagtacgtg tcgttcctga
     9781 tcggcctcaa gcagtacgcg gggaactggg ccgcgaccac ctggtcgttc cgtgacaagg
     9841 agaaggaaga tcgcctcaac gagcgcatcg tcaaagcggc cgacaaccag atcgatcaga
     9901 tcgagccact gttcggtaag gagatctccg agatcttcat ccaaaaggcg atcgccttcc
     9961 ggatgatgca cccgatgggg cggatgcaca tcacggccca tatgcgtcat agcggtgaca
    10021 tcgatacgcg ggtgttgcgc gagggagagt tcgtcagcaa cgtgttgacc gtcttgaact
    10081 tcggcgatgg acactgccac gacgaacgac tgatcgacgc ggtgcaacag cgctgcaact
    10141 acgagccggg cgatctcgtg gtgtgcttca ccgagagtca gccgatgttc tccaagaagg
    10201 tgcaataccg tgtcatcgat gcggcagtcg gtgtggtcga gaagggttgg taccacaacg
    10261 acgatgccgc caacgagcaa ccgtggttgc ccaatgggct actgccccac acggtcactt
    10321 ggacccgcga cggctacgtc gcgccgggcg cgccctaccc tggcaacggt ggtcgttctt
    10381 gagcagacgg atgtgaaggg gtgacccggg cggtcgtcgt cggctccggg ccgaacggtc
    10441 tggccgctgc ggtggcgttg gcacgcgccg ggatcgaggt cacggtgttc gagcgagccg
    10501 acacgatcgg cggtggtacc cggagcagcg acgcgatcgt gccggggctc atccacgacc
    10561 attgttcgtc cgcacacccg atggcgatcg cctcgccgtt cttccagcag atgcagttgc
    10621 cagggatcac ttggcgcgcg gccgagatcg actgcgccca cccgctcgac gacggttccg
    10681 ctggtgcctt cgcgcgctcg ttcgatgtca ccgccgatgc gctcggcgcc gatggacgcc
    10741 gttggcggcg gatgttcgag ccgatcgccc gnnnnnnnnn nttgatcatc gcgggtttta
    10801 gtccggtacg atccgccgct tcgacgatct gttcgcagac atctcccagc cgttgttgcg
    10861 cgtgccgcgc cacccgctca cgctggcccg gtttggtatg cgcgccctgc cgccagccac
    10921 ggtgctggcc cgggccttgt cgacacctca ggcgcgggcg ttgtggacgg gcgtcgccgc
    10981 gcatgcattc tggcccctgg gttcgccgct gacgagcgcc gtcggggttg ccctcacggc
    11041 gggcgcgcac gcgcatgggt gggtcgtggc cgagggcgga tcgcaggcga tcgccgacgc
    11101 cctcgcggcc gacgtcgtgg cgcacggcgg ccggatcgag acgggggtcg aggtcacctc
    11161 ggtcgaacag ctcggcgggc ccgacgtggt catgctcgac gtgtcgccgt cggtggcgtt
    11221 ggccatcctc ggtgatctgc tgccgtcgcg ggtcgcgcgg gcctatcgcc ggttccgcca
    11281 cggtccgggg gcgttcaagg tcgacttcgc gatcgacggc gatgtgccct gggtggcgga
    11341 ggtcgctcgc cgcgcgggca cggtgcatct cggcgggccg atggcggaga tcgttgccgc
    11401 cgagcgtgat ctgcatcgtg gccggatgcc cgagcggccc ttcgtgctcg tcggccagca
    11461 cggccaacga ccaggcggcc gcggcggcct gctctccatc agggaagtcg gtcaggtagg
    11521 cgtcataggc ggccaaagca tct
//
LOCUS       DAORNF010000012        11482 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_4248_length_11482_cov_1.606371, whole genome shotgun sequence.
ACCESSION   DAORNF010000012 DAORNF010000000
VERSION     DAORNF010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 11482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11482
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4248_length_11482_cov_1.606371"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="PLV68_01030"
     CDS             <1..552
                     /locus_tag="PLV68_01030"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HRD99847.1"
                     /translation="RTNPNNYHGMLRLDHNRAVTQLAQKVGRPVSAVTKMTIWGNHST
                     TQYPDLFHAEVDGNNAYELVGDHAWVDGTFIPTVAKRGAAIIEARGASSAASAANAAI
                     DHIRSWHLGTAEGDWVSMAIPSDGSYGVPEGLISSFPCVCKDGAYSIVQGLEIDDYSR
                     GKIDASTGELSEERDAVRELGLI"
     assembly_gap    592..707
                     /estimated_length=116
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1048..>1891)
                     /locus_tag="PLV68_01035"
     CDS             complement(1048..>1891)
                     /locus_tag="PLV68_01035"
                     /inference="COORDINATES: protein motif:HMM:NF019368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HRD99848.1"
                     /translation="PQLEQLRDRALAALATATGGAAVLANSAPHARHEVTCLPADLIA
                     RSGMADGQHLSNGLVAAWVAIDATTVTPLDAAVIAAADRSAPVGVTETDDRSIVIDNG
                     VVAVTIDGDGLITSVWDHRAEREVILDGQRANVLELSPDTPIEYDAWDLDDYYANSVT
                     EITDVDAIEIVDRGPLVAAVTVRRSFGRSQVIQTIVVRAGSARIDVITDIDWYEDDHV
                     LKVAFPVDVHADTAACEVQYGHTMRPTHASTSWDAAKFEICAHRFVDVSEHGYGVALL
                     NDCK"
     assembly_gap    1892..2165
                     /estimated_length=274
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2166..>2456)
                     /locus_tag="PLV68_01040"
     CDS             complement(<2166..>2456)
                     /locus_tag="PLV68_01040"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99849.1"
                     /translation="WWQGIDGTAIFTHFPPVDTYNVSMRPFELAHAERNYADHGGGTR
                     SMAPFGFGNGGGGPTRDMMERYRRMRDLEGLPRVDIAAPADWFPQAIDDYPD"
     assembly_gap    2457..2466
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2467..>3611)
                     /locus_tag="PLV68_01045"
     CDS             complement(<2467..>3611)
                     /locus_tag="PLV68_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99850.1"
                     /translation="SAPFTVTAYPVDGEPISFDDVMLAMADGLFRPFAIGDPYGPPWS
                     TTWFHCVGRVPHEWAGSTVEARIDLGFGQAPGFQAEGLVWGPSGPLRALNPLNHAIPL
                     LSEARGGEEFEFLVEAAANPMIGGPDHRPTPLGDPRTLPSTPLYTLKRAELSVFQPEV
                     AALVHDLDVLGELIRELPSNAPRRPQITVAIGRALDALDLGDVPATASDARAELTKVL
                     ARPAVPSAHHITAVGHAHIDTAWLWPLRETVRKCARTFSNALDLMTREPELVFVCSQA
                     QQHEWMRERYPSIFTDMQARAGEGRFVPVGGMWVEADMNLPSGESLARQFLRGQRFFE
                     EHYGEICREVWIPDVFGYPANAPQIFRLGGAEWFLTQKLSWNKQNRMP"
     assembly_gap    3612..3621
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3792..4139)
                     /locus_tag="PLV68_01050"
     CDS             complement(3792..4139)
                     /locus_tag="PLV68_01050"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HRD99851.1"
                     /translation="MVRRGDVYWSDLGALEGSAPTKRGPVLIVQSDEYNRSRLATTIV
                     VALSSNTALAAMPGNVFLPAVVTGLPKDSVANVTALATVDRRTLDPHPVGSVPDHVMA
                     ELDHSLRRALALP"
     gene            complement(4133..4402)
                     /locus_tag="PLV68_01055"
     CDS             complement(4133..4402)
                     /locus_tag="PLV68_01055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99852.1"
                     /translation="MKTAISVPDATYEQVEHAALRHGMNRSQFYARAAEHYIAYLDSA
                     DLTASIDAAIEALHDAAEADESTVFAVAAGHHTLSSETPSDDESW"
     gene            4512..>4703
                     /locus_tag="PLV68_01060"
     CDS             4512..>4703
                     /locus_tag="PLV68_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99853.1"
                     /translation="MPVHLRAEPSDYADAVLVPGDPRRARYIAETFFDAGARCVNEER
                     GALGFTGTFQGKPTLLNVWA"
     assembly_gap    4704..4713
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4714..5268
                     /locus_tag="PLV68_01065"
     CDS             <4714..5268
                     /locus_tag="PLV68_01065"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99854.1"
                     /translation="KPISVQSVGMGGPSAAIYYTELFQLGAKRLIRVGTAGGLGDDMR
                     MGDTIVALSATPDDPTTAVLTNGEAHAPTATYALVERAVQLARAQGGRVHVGAVVTSA
                     LFYDTRQGMMQRWRERGHLGVEMEVSILYTLAAIHQREALALCTVSDLILDSGEAERI
                     SDDELKRGVDQMMRIACEVATSDS"
     gene            complement(5417..>5756)
                     /locus_tag="PLV68_01070"
     CDS             complement(5417..>5756)
                     /locus_tag="PLV68_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99855.1"
                     /translation="AEQAADAEARIRALLAWRDSTLTMLRTTRVKGVAIAVTEKLIEY
                     PTVTVKNIAESHNVSAQAANNAVTRLVDLGVLSEITGRNYNRVFSAPAVFNILFRGDA
                     FRARMTRPES"
     assembly_gap    5757..5890
                     /estimated_length=134
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5891..6549)
                     /locus_tag="PLV68_01075"
     CDS             complement(<5891..6549)
                     /locus_tag="PLV68_01075"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HRD99856.1"
                     /translation="MPDPLPSTLELSARTWSDVANAAHLLGRLDALAGELLPNPMLVA
                     RPTIRREAVSTSALEGTFAPAAEVLSSEIDEELPRSHAVTEVINFIRATEHAIERRAT
                     LPISTRLACELQRILVVGTASEDWQAGQVRQTQVIIGPYKGCSVLEASYIPPPPGELL
                     NDGLSAWETWIHAESDLHALIRVAAAHYQFEALHPFTDGNGRIGRLIAILQLIDYGLL
                     T"
     gene            6972..>7358
                     /locus_tag="PLV68_01080"
     CDS             6972..>7358
                     /locus_tag="PLV68_01080"
                     /inference="COORDINATES: protein motif:HMM:NF016220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease"
                     /protein_id="HRD99857.1"
                     /translation="MSEGYLTAEARARVEIDRQLVACGWVVQDRKDLNLYAGQGVAVR
                     EFIMATGHGRADYLLFVDGKAVGAIEAKPSGTPLAGVESQSAKYAAGLPKDLPALVSP
                     LPFLYESTGDETMFTDGFDPEPRSRSV"
     assembly_gap    7359..7469
                     /estimated_length=111
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7484..9226
                     /locus_tag="PLV68_01085"
     CDS             7484..9226
                     /locus_tag="PLV68_01085"
                     /inference="COORDINATES: protein motif:HMM:NF037510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HRD99858.1"
                     /translation="MRRIQVEAIGRIEASMRRNHPRALAQMATGSGKTFMAANLAYRL
                     VQTAGAERVLFLVDRANLGRQTLREFQQFATPGDGRKFTDLYNVQWLTSNRIDPAARV
                     VITTVQRLYSMLRGEEELSPDVDELSGSEIEPERPVEIAYNSKIPIETFDVVIVDECH
                     RSIYGVWRQVIEYFDAYLVGLTATPAKQTFGFFNQNLVVEYGHAEAVADGVNVDFDVY
                     RIRTKITEKGSTVEAGLVTKFRDRETRKTRLEKLDEDVVYDARQLDRDVVALDQIRTV
                     IRTFRDNLYKPLEEGGIFPGRSEVPKTLIFAKDDSHADDIVRICREEFGRGNDFAVKI
                     TYRTTGVKPEDLLNLFRNSFNPRIAVTVDMIATGTDVRPLECVMFMRMVRSRNFFEQM
                     KGRGVRIIDPNDLQAVSADATAKTHFVIIDAVGVTEAEFNDTQPLERKKGESLKKLLD
                     QVAAGVRDADVVSSIAGRLARLDRVISHDDRRQLADAADGIDLTDIVRVLTNAVDIDV
                     AWERAASANGGDDPTDEQLADARSEMIEKASRVLAERPALREKLIEVRRSYTQVLDET
                     SKDEIIATPPTAHA"
     gene            9280..>9586
                     /locus_tag="PLV68_01090"
     CDS             9280..>9586
                     /locus_tag="PLV68_01090"
                     /inference="COORDINATES: protein motif:HMM:NF020052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification enzyme R subunit
                     C-terminal domain-containing protein"
                     /protein_id="HRD99859.1"
                     /translation="MQVLYSRPYGKRLTLKAIKELAATIGRPPYNWTPERLWAAYEAL
                     ESQRVRGSAGTVLTNLVSLVRHALEPDGELVAYPLAVEGRFQNWLAQQTQAGTAFTDD
                     "
     assembly_gap    9587..9824
                     /estimated_length=238
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            10087..>11135
                     /locus_tag="PLV68_01095"
     CDS             10087..>11135
                     /locus_tag="PLV68_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99860.1"
                     /translation="MLSGKMYRFRVDERLIDPRFIEAMLCSPDLQIALDQIKTGGSDS
                     GLNLTHERFRSLRLPLPPSAEQIRIVTVIEEAFSKLDAGEAGLRTVRQLLKRMREAVL
                     AAAVGGRLVPQDPADIPATEILADLGVEPDSSTDDLPLGWVATALGGLLRGIEAGKSF
                     ATQGQPARSGEMGVIKVSAMTWGEFRPEENKALPPGTHIEQRWVVRAGDLLLSRANTE
                     EYVGACVLVPTDHLDLILSDKSLRLVPADGIEPRWLLHALRSRPARVQIEQLATGTKE
                     SMRNISQAKLRGLRLSLPPTAEQRRIADEIERQLSFIDACVQAIDTATERGVALRSSV
                     LKAAFEGWLVPQDPT"
     assembly_gap    11136..11166
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            11227..>11482
                     /locus_tag="PLV68_01100"
     CDS             11227..>11482
                     /locus_tag="PLV68_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99861.1"
                     /translation="MMGQGSETSPTNHGLLKSPVSTDVLVLGAGFSKAVSGRFPLTDD
                     LGTSAMALAGVSRDRPFKDGRFEAWLSQLAEPQPYLSQAEN"
BASE COUNT         1801 a         3418 c         3555 g         1774 t
ORIGIN      
        1 cggaccaacc ccaacaatta ccatggaatg cttcgcctcg accacaaccg ggccgtgacc
       61 cagttggcgc agaaggtcgg ccggccggtc agcgccgtga cgaagatgac gatctggggc
      121 aaccactcca ccacgcagta ccccgacctg ttccacgccg aagtcgacgg caacaacgcg
      181 tacgagctcg tcggcgatca cgcgtgggtg gacggcacgt tcatccccac ggttgccaag
      241 cgcggcgcgg ccatcatcga agcccgcggc gcatcgtcgg cggcgtcggc ggccaacgcg
      301 gccatcgacc acatccgctc gtggcacctc ggcaccgctg agggcgactg ggtcagcatg
      361 gcgatcccgt ccgacggcag ctacggcgta cccgagggcc tgatctcgtc gttcccgtgc
      421 gtgtgcaagg acggcgcgta cagcatcgtt cagggcttgg agatcgacga ctacagccgc
      481 ggcaagatcg acgcctccac cggcgaactg tccgaagagc gcgacgctgt ccgcgaactc
      541 ggcttgatct gaccggccag cccgcgtcag cggcgcaggc gcagcgtcac gnnnnnnnnn
      601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntca acactgtggt
      721 ctggcgcccc ccggccgctt cgtagcagcg cacgatcacg tcgccgctgc cgtcttcggc
      781 gggcttgacc gcggtgacga cgacgtgggg gttggacgat gcgaccgcgg tgaccggccc
      841 gcctgccgcg ccgcctgacc cggcgacggt caccagcggc tggttgagcc gggccgcctc
      901 ggcgacgacc ccgccgtcgg ccagaccacc ctggtggggg aacagcgcgt acgtgacgcg
      961 gtggtggcca cggtcggcct gtgggtccgg gtaaccagcg gccttgagca gtgtcaggcg
     1021 cagcgcgcca cgctgaatgt cgtggcccta cttgcagtcg ttcaagaggg cgacgccgta
     1081 gccgtgctcg ctgacgtcga cgaagcgatg ggcgcagatc tcgaacttgg ccgcgtccca
     1141 actggtgctg gcgtgggtgg ggcgcatggt gtgcccgtac tgcacctcgc acgcggcggt
     1201 gtcggcgtgg acgtcgaccg ggaaggcgac cttgagcacg tggtcgtcct cgtaccagtc
     1261 gatgtcggtg atcacgtcga tgcgggccga tccggcgcgc accacgatcg tctggatcac
     1321 ctgcgagcgg ccgaacgagc ggcgcacggt gaccgcggcg acgagcgggc cacgatcgac
     1381 gatctcgatc gcatcgacgt cggtgatctc ggtgaccgag ttggcgtagt agtcgtccag
     1441 gtcccacgcg tcgtactcga tcggtgtgtc cggcgagagc tccaacacgt tggctcgttg
     1501 gccgtcgagg atgacctcgc gttcggcgcg gtgatcccac accgacgtga tcagcccgtc
     1561 gccgtcgatg gtcacggcga cgacgccgtt gtcgatcacg atcgaacggt cgtcggtctc
     1621 cgtgacgccg accggcgcgg accggtccgc cgcggcgatg accgcggcat cgagcggtgt
     1681 caccgtcgtc gcatcgatcg cgacccacgc ggcgaccaga ccgttcgaca ggtgctggcc
     1741 atcggccatg ccgctgcgcg cgatgaggtc ggcgggtaga cacgtcacct cgtggcgagc
     1801 gtggggtgcg gagttggcga gcacggccgc tccgccggtc gccgtcgcca acgcggcgag
     1861 cgcccggtcg cggagctgct ccaactgggg gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnncgtcc gggtagtcgt cgatcgcctg cgggaaccag tcggcgggtg cggcgatgtc
     2221 gacgcgcggc aggccctcca gatcgcgcat ccgtcggtag cgctccatca tgtcgcgggt
     2281 cgggccgcca ccgccgttgc cgaaaccgaa cggcgccatc gacctggtgc cgccgccgtg
     2341 atcggcatag ttgcgttcgg catgggccag ctcgaacggc cgcatcgaca cgttgtaggt
     2401 gtcgaccgga ggaaagtggg tgaagatcgc cgtgccgtcg atgccctgcc accagannnn
     2461 nnnnnnggca tgcggttctg cttgttccaa ctgagttttt gggtgaggaa ccactcggcc
     2521 ccgccgaggc ggaagatctg cggcgcgttg gccgggtagc cgaacacgtc gggaatccac
     2581 acctcgcgac agatctcccc gtagtgctct tcgaagaagc gttgcccgcg caggaactgg
     2641 cgggccagcg attcgccaga gggcaggttc atgtcggctt cgacccacat cccgcccacc
     2701 ggcacgaacc ggccttcgcc ggcgcgggct tgcatgtcgg tgaagatcga cgggtagcgc
     2761 tcgcgcatcc attcgtgctg ctgagcctgg ctgcagacga acaccaactc gggttcgcgg
     2821 gtcatcagat cgagcgcgtt ggagaacgtg cgagcgcact tacgcaccgt ctcgcgcaac
     2881 ggccacagcc acgcggtatc gatgtgggcg tggccgacgg cggtgatgtg gtgggcactc
     2941 ggcaccgcgg ggcgagcgag taccttcgtg agctcggcgc gtgcatctga cgctgtggcg
     3001 ggaacgtcgc cgaggtcgag cgcgtcgagt gcccgcccga tcgcgaccgt gatctgcggt
     3061 cggcggggcg cgttggaagg gagttcacgg atcaactcac cgagcacgtc caggtcgtga
     3121 accagcgcgg cgacctcggg ctggaacacg ctcagctcgg ctcgcttcag cgtgtagagc
     3181 ggtgtggacg gcaaggtgcg agggtcgccg agcggcgtcg ggcggtggtc gggcccaccg
     3241 atcatcgggt tggccgcggc ctcgaccaag aactcgaact cctcgccgcc gcgagcttcg
     3301 gacagcaacg gaatggcgtg gttgaggggg ttgagggcgc gcagcggccc gctcggcccc
     3361 cacaccagcc cttcggcctg aaagcccggt gcctggccga agccgaggtc gatccgcgcc
     3421 tcgacggtgc tgcccgccca ctcgtgcggc acccggccga cgcagtggaa ccaggtggtg
     3481 ctccacggcg ggccgtacgg gtcgccgatc gcgaacggcc ggaacaaccc gtctgccatc
     3541 gccagcatca cgtcgtcgaa gctgatcggc tcgccgtcga ccggatatgc cgtgaccgtg
     3601 aacggcgccg annnnnnnnn ngtccgccat ggggacaagc acggcaagca cgggacggat
     3661 gcgctcgcgc aggaatcgtt gcaagcgctt ctcggtgatc agatggcggt cgtgcacgcc
     3721 cgccattctg accgccccgc ccgcctccca ccggtacagt cggctccctt tgctggcggt
     3781 tgcactcggg atcagggcag agcgagggcg cggcggaggc tgtggtcgag ttcggccatg
     3841 acgtgatcgg ggacagagcc gaccgggtga gggtcgagcg tgcgccggtc gacggtggcg
     3901 agcgcggtga cgttggcgac ggagtccttg ggcaggccag tgacgaccgc gggcaggaac
     3961 acgttgccgg gcatcgctgc cagcgcggtg ttggaactca acgccaccac gatggtggtt
     4021 gcaagtcggc tgcggttgta ctcgtcggac tgcacgatga gcaccggccc ccgcttcgtg
     4081 ggggcgctgc cctcgagcgc gccgaggtcg ctccagtaca cgtcgccgcg tctcaccatg
     4141 actcgtcgtc gctcggagtt tcactgctca gggtgtgatg gcctgcggcc accgcgaaca
     4201 cggttgactc atccgcctca gcggcatcgt gcaatgcctc gattgcggcg tcgatcgagg
     4261 cggtgagatc ggcggagtcg agatatgcga tgtagtgctc ggctgcccgc gcgtagaact
     4321 gcgaacggtt catcccgtgc cggagcgcgg cgtgctcgac ctgttcgtac gttgcgtccg
     4381 gcactgagat cgcggtcttc accccagcga gtataacgag gtataccctc ggatcgaccg
     4441 ttgccttggt cgatggttga ccgacctttg gttgggtgag tggggggtgg gcgggatcgt
     4501 aaggtgggcc gatgcctgtg catcttcgtg ccgaaccgtc cgactatgcc gatgcggtgt
     4561 tggtgcccgg tgacccgcgc cgggcgcgct acatcgccga gacgttcttc gatgccggag
     4621 cgcgttgcgt caacgaggag cggggcgcgc tcgggttcac cggcacgttc cagggcaagc
     4681 cgaccctctt gaatgtctgg gcgnnnnnnn nnnaagccga tcagcgtcca gtccgtcggc
     4741 atgggcgggc cgtcggccgc gatctactac accgagctgt tccaactggg cgccaagcgg
     4801 ctgatccgcg tcggtaccgc cggaggcctc ggcgacgaca tgcggatggg cgacacgatc
     4861 gtcgccctca gcgcgacccc cgacgacccg accaccgcgg tgctcaccaa cggcgaagcg
     4921 cacgcgccga ccgcgacgta cgcactggtc gagcgagcgg tgcagctggc ccgagcccag
     4981 ggcggacggg tacacgtcgg cgcggtggtg accagcgcgc tgttctacga cacccgccag
     5041 ggcatgatgc agcgctggcg cgagcgtggt catctcggag tcgagatgga ggtctcgatc
     5101 ttgtacaccc tcgccgcgat ccaccagcgc gaggccttgg cgctgtgcac ggtgagcgat
     5161 ctcatcctcg acagcggtga agccgaacgg atcagcgacg acgaactcaa gcgcggcgtc
     5221 gaccagatga tgcgcatcgc ctgcgaagtc gccaccagcg actcgtagag agcctggttt
     5281 cgaacgtcgc gacgcgtcca tccggacgct cgatgagttc atcgttggcc catctgcaca
     5341 tgtgcagacc gtgctgggcg ttcaccgaac agcgagcggt aattcgtcac ctggcgtcgg
     5401 gtcagtcaca ttgcggtcag ctctccggcc gagtcatccg cgcccgaaag gcgtcaccgc
     5461 ggaacaggat gttgaacact gcgggtgcgc tgaacacgcg gttgtagttt cgtcccgtga
     5521 tctcgctgag caccccgaga tcgacgagtc gagttactgc gttgttggcc gcttgggcac
     5581 tgacgttgtg gctctccgcg atgttcttga cggtcaccgt tgggtactcg atgagcttct
     5641 ccgtcacggc gatggcgacg cccttgactc gtgtcgtgcg cagcatggtg agcgtggagt
     5701 cccgccacgc gagcagcgcg cgtatccgcg cctctgcgtc cgcagcttgt tcagcgnnnn
     5761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5881 nnnnnnnnnn tcggtgagca gcccgtagtc gatcagttgc aggattgcga tcaaacggcc
     5941 gatgcggccg ttgccgtcgg tgaacgggtg cagggcctcg aactggtagt gggcagccgc
     6001 cacccgaatg agggcatgga ggtcactttc ggcgtggatc cacgtctccc acgccgacag
     6061 gccatcgttg agaagttcgc ctggcggtgg cgggatgtag ctcgcctcga gaacactgca
     6121 gcccttgtag ggaccgatga tgacctgcgt ctggcggacc tgacctgcct gccagtcctc
     6181 actcgcagtc ccgacgacga ggatgcgctg cagctcacac gcgagccgag tgctgatcgg
     6241 gagcgtggcc cgccgctcga tggcgtgttc ggtcgcacgg atgaagttga tgacctcggt
     6301 gacggcgtgg cttcgaggca gttcctcgtc gatctccgaa ctcagcacct ccgcagcagg
     6361 ggcaaaggtg ccttcgaggg ctgaagtgct caccgcctca cgccggatcg tggggcgcgc
     6421 aacaagcatc gggttgggca acaactcccc tgcgagtgcg tcgagcctgc ccaggaggtg
     6481 cgctgcgttg gcaacgtcgg accacgttcg cgccgagagc tcaagcgtcg atgggagcgg
     6541 atcgggcacg aacgctgagt ggtcgacctg tcgatcagcc tcgtcgacgc tgatcggaac
     6601 gagcctgcca gttgccccgc gtccgaaccg cttcagatcc acaggtcaag gatagggcca
     6661 tcaaccttga gtttcacctc tgtgatttca agtggggccg tggctggcgg tcacggtcaa
     6721 tcttgatggc atcaaccttg agaacttgcc tgtggatttc aagtttcgct tccgtcttgc
     6781 cgctccagcg cgttggagcc tgcggtcagc aggatccgac cagttccggt cgagccggtg
     6841 gtgctggaac accgaccttc accgcgctcg ggcagttgac gataggtgtg ctttcggcgg
     6901 caacccgata ggtggtcgtg ggccgcctac cctgttggga ccatgtggct ctctcggctc
     6961 tggaccctgc gatgagcgag ggatacctga ccgcggaggc gcgcgcccgc gtggagatcg
     7021 atcggcagct cgtggcgtgc gggtgggtgg tgcaggaccg caaggacctg aacctgtacg
     7081 ccgggcaggg ggtggcggtc cgggagttca tcatggccac cggtcacggc cgggcggact
     7141 acttgttgtt cgtggatggc aaggcggtcg gggcgatcga ggccaagcca tcgggcacgc
     7201 cgctggcggg ggtggagtcg cagtcggcga agtacgcagc cgggttgccg aaggacctgc
     7261 cggcgttggt gtcgccgtta ccgttcctgt acgagtcgac cggcgacgag acgatgttca
     7321 ccgacgggtt cgacccagag ccccggtccc gttcggttnn nnnnnnnnnn nnnnnnnnnn
     7381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt accggcggac gggttgcggc ggatccaggt
     7501 cgaggcgatc ggccggatcg aggcgtcgat gcgccggaac catccgcgcg ccttggcgca
     7561 gatggcaacc gggtccggga agacgttcat ggccgccaac ctcgcctacc ggctggtgca
     7621 gaccgctggt gccgagcgcg tcctgttcct cgtcgatcga gcgaacctgg gccggcagac
     7681 gttgcgcgag ttccagcagt tcgcaacccc cggcgacggt cgcaagttca ccgatctcta
     7741 caacgtgcag tggctcacct cgaacagaat cgacccggcg gcacgcgtgg tgatcaccac
     7801 cgtgcaacgg ctgtattcga tgctgcgtgg cgaggaggag ctgtcacccg atgtcgatga
     7861 gctgtccggg tcggagatcg agccggagcg accggtcgag atcgcttaca actcgaagat
     7921 tccgatcgag acgttcgacg tggtgatcgt cgatgagtgt caccggtcga tctacggggt
     7981 gtggcggcag gtgatcgagt acttcgacgc ctatctggtc gggctgacgg cgacgccggc
     8041 gaagcagacc tttgggttct tcaatcagaa cctcgtcgtc gagtacggcc acgccgaagc
     8101 agttgccgac ggcgtgaacg tcgacttcga cgtgtatcgc attcggacca agatcaccga
     8161 gaagggctcc accgtcgagg ccgggctcgt cacgaagttc cgcgaccgcg agacccgtaa
     8221 gacccgcctt gagaagctcg acgaggacgt cgtctacgac gcccgccaac tcgaccgtga
     8281 cgtcgtcgcc ctcgaccaga tccgcacagt catccgcacg ttccgcgaca acctctacaa
     8341 gccgctcgaa gaaggtggca tcttccctgg ccgcagcgag gtacccaaga cgctgatctt
     8401 tgccaaggac gacagccacg cggatgacat cgtgcggatc tgtcgcgagg agttcggtcg
     8461 tggcaacgac ttcgccgtga agatcaccta ccgcaccacc ggggtgaagc ccgaggacct
     8521 gttgaacctg ttccgcaaca gcttcaaccc gcgcatcgca gtcacggtcg acatgatcgc
     8581 caccggcacc gacgtgcgcc cgctggagtg cgtgatgttc atgcgcatgg tccgctcccg
     8641 gaacttcttc gagcagatga agggccgcgg ggtacgaatc atcgacccca acgatcttca
     8701 ggccgtgtcg gcagacgcga ccgcgaagac gcacttcgtg atcatcgacg ccgtcggcgt
     8761 caccgaggcc gagttcaacg acactcaacc actcgaacgc aagaagggcg agtcgctcaa
     8821 gaaactcctc gaccaagtcg ctgccggtgt ccgcgatgct gacgtggtct cgtcgatcgc
     8881 cggccgcctc gcccgactcg atcgagtgat ctcccacgac gaccgcagac aactcgccga
     8941 cgccgccgac gggatcgatc tcaccgacat cgtccgcgtg ctgacgaacg ctgtcgatat
     9001 cgacgtcgca tgggaacgag ccgcatccgc gaatggcggc gacgatccga ccgacgagca
     9061 gctcgccgat gctcgttccg agatgatcga gaaggccagc cgcgtcctcg ctgaacggcc
     9121 ggcacttcgg gaaaagctga tcgaggttcg ccgcagttac acgcaggtgc tcgacgagac
     9181 atccaaggac gagatcatcg cgacgccacc gaccgcgcac gcctgacggt tgagtcatgg
     9241 cgggccttcg tcgccgaaca caaggacgac atcgacgcgt tgcaggtcct gtactcccgc
     9301 ccatacggca aacggctgac gttgaaggcg atcaaggagc ttgccgccac catcggtcga
     9361 ccgccgtaca actggacacc cgaacgcctt tgggccgcct acgaggcgct cgaatcccaa
     9421 cgcgttcgcg ggtccgcggg gaccgtgctg accaacctcg tgtcgcttgt gcgtcacgct
     9481 ctcgaacccg atggtgagct cgtcgcgtac ccactcgccg tcgaagggcg cttccagaac
     9541 tggctcgccc aacagaccca ggccggcacc gccttcactg acgaccnnnn nnnnnnnnnn
     9601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngggtga tggccgattg
     9841 gtccaccaag gctggagccc acagtgcgaa cgcacaccca catctggcga tcacgagtgg
     9901 ggggtcctca agacgacagc gatccaggcc ggggcctttc tcccgcatga gcacaaacgg
     9961 ctacccgact cactggagcc gcggtccgtc atcgaggtgc ggcaaggtga tctgcttctc
    10021 acctgcgcgg gcccccgcgc ccggtgcggc gtcgcatgct tggtgcgcag cagtcggcct
    10081 catctgatgc tctccgggaa gatgtaccga ttcagagtcg acgaacgtct cattgatccc
    10141 aggtttatcg aagcgatgct gtgcagcccc gatctccaga tcgcgctgga ccagatcaag
    10201 acgggcggca gcgacagcgg actgaacttg acgcacgaac ggttccgatc gttgcgactg
    10261 ccgctgccgc cgagcgccga gcagatacgg atcgtcacgg tgatcgagga ggcattctcc
    10321 aagctcgacg ccggcgaggc tggactccga acagtccgcc aactcctcaa gcgcatgcga
    10381 gaagccgtcc ttgctgccgc cgtcggcggc cgccttgtcc cccaagaccc cgccgacatc
    10441 cccgccacag agatcctcgc tgatcttggg gtcgaacctg acagctcgac tgacgacttg
    10501 ccgctcggat gggtcgcaac agccctcggc ggtttactgc ggggcatcga ggcaggcaag
    10561 tcgttcgcta cgcagggcca acctgcccgg tctggagaga tgggggttat caaggtcagc
    10621 gccatgacgt ggggcgagtt ccggcccgaa gaaaacaagg cgttgccgcc cgggacgcac
    10681 atcgaacagc ggtgggtcgt gcgggccggc gacctcctcc tctcccgggc aaacaccgag
    10741 gagtacgtcg gcgcgtgtgt gctggtgccg acggatcacc ttgatctgat tctcagcgac
    10801 aagagccttc gccttgtccc ggcagatggc atcgagccga ggtggttgct tcacgccctg
    10861 aggtctcgtc ctgccagagt ccagatcgaa caactcgcga cgggcaccaa ggaatcgatg
    10921 cgcaacatct cacaggcgaa gctccgcggt ctccgactgt cccttccccc aaccgcggaa
    10981 caaaggcgga ttgccgacga gatcgaacga caactgtcgt tcatcgacgc ctgcgtgcag
    11041 gccatcgata ccgccaccga gcgaggcgtc gcactgcgca gttcggtatt gaaggcggcg
    11101 ttcgaaggtt ggctcgtgcc ccaggacccg accgannnnn nnnnnnnnnn nnnnnnnnnn
    11161 nnnnnntcgt caggtcgact ggcgggcgac cgacacgcgg cggcgctcat cgcaggatgt
    11221 ccggcagtga tgggccaggg ttcggaaacg tcaccgacca accatggtct tctgaagagc
    11281 cccgtcagca ctgacgtgtt ggtgctgggc gctggattct cgaaggccgt cagcggccgc
    11341 tttccgctca ccgacgatct cgggacgagc gccatggctc tggcaggtgt aagccgggat
    11401 aggcccttca aggatggtcg tttcgaggcg tggctctccc aactggcgga accgcaaccg
    11461 tacctgtcgc aggccgagaa ca
//
LOCUS       DAORNF010000013        11285 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_4327_length_11285_cov_2.023954, whole genome shotgun sequence.
ACCESSION   DAORNF010000013 DAORNF010000000
VERSION     DAORNF010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 11285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11285
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4327_length_11285_cov_2.023954"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..620
                     /locus_tag="PLV68_01105"
     CDS             <1..620
                     /locus_tag="PLV68_01105"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HRD99862.1"
                     /translation="SDGTPEEVAEEVARMQAVLASSGASDLRVSQSEAERLRFWAGRK
                     AAFPAVGRITPDYYCMDGTIPRKRLSEMLQAIAEMERKYGLRCANVFHAGDGNLHPLI
                     MYDAAVPGELHRAEEFGAEILELSVALGGSITGEHGVGIEKINQMCVQFATPELESFH
                     RVKAAFDEAGLLNPGKAVPTLHRCAEYGRMHVHHGETKFPELDRF"
     gene            620..1675
                     /gene="glcE"
                     /locus_tag="PLV68_01110"
     CDS             620..1675
                     /gene="glcE"
                     /locus_tag="PLV68_01110"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: protein motif:HMM:NF008439.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HRD99863.1"
                     /translation="MTLDDLVARVAAAAEAGTALRVRGGGSKDFYGGMLAGELLDVSG
                     HRGILAYEPTELYLTARAGTPLAEVEAALAERGQMLPFEPPHFGSATVGGCVAAALAG
                     PRRQQAGGVRDYVLGVRMIDGRGQLLNFGGQVMKNVAGFDLARLLTGSLGTLGVLAEV
                     TFKVLPRPVAETTLHFELSQAEALEQLNRWGGQPLPISGSFWHEGQLTVRLSGAHAAV
                     DSARRILGGEHVPQAEGWWQSVREQTHAAYAADRLWRLALPTTAPVLDLPGEVAVEWG
                     GGLRWLRSDAEVAVVRQAAAEVGGHATLYRAPESLRCLENAFTPLPLAVLALHQRLKK
                     AFDPKGIFNPGRLFAEV"
     gene            1681..>2490
                     /gene="glcF"
                     /locus_tag="PLV68_01115"
     CDS             1681..>2490
                     /gene="glcF"
                     /locus_tag="PLV68_01115"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: protein motif:HMM:NF008434.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HRD99864.1"
                     /translation="MHSELADFIRDTPDGQEAAAILKKCVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGKPATAKTQLHLDRCLTCRSCETTCPSGVKYSHLLDIGRKVV
                     DAQVPRRLGASVVRLALKTALPRAWIFKPAVKLGQLMRPLLPALLAEKMPAAVAGSRR
                     PWPEAKAHPRRMLMLAGCVQPALAPSINVAMARMLDRLGISPVEATGAGCCGALKFHL
                     NDHAAALDDMRRNIDAWWPHVEAGAEAIVVTASGCGVQVKDYGHALRHDAEY"
     assembly_gap    2491..2500
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2501..2923
                     /locus_tag="PLV68_01120"
     CDS             <2501..2923
                     /locus_tag="PLV68_01120"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HRD99865.1"
                     /translation="KAEHISKLCRDPSEILAAEKERLLPLLTGVKPAREKLAFHSPCT
                     LQHGLQIRGAVEEVLGAAGFSLTPVKDAHLCCGSAGTYSVLQPDLSHQLRDNKLAALG
                     AGGPERIATANIGCLTHLQAGSTTPVVHWIELIDESLP"
     gene            2920..>3184
                     /locus_tag="PLV68_01125"
     CDS             2920..>3184
                     /locus_tag="PLV68_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99866.1"
                     /translation="MTLTPVADIVKTLDAPDAAMVKRLLERGMMIPVQPRVLEELRQQ
                     MLKKEFDIRVLSRIINSDPGIVAMLFKACGNSAYRQHQPFQSVD"
     assembly_gap    3185..3215
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3216..3803
                     /locus_tag="PLV68_01130"
     CDS             <3216..3803
                     /locus_tag="PLV68_01130"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HRD99867.1"
                     /translation="NLIQAIAVASLHDSKQNSEAYESFWVRSQAVAQLAMIVADERIS
                     VCNLFPDQAYLAGMFHDCGVALLMRRFSTYCKEMKLGEPGRWIDLAEEDAKFKADHCV
                     VGYLIARHWNLPDFICDAIRNHHDIQRIGSSHASRSMVAILQLAIHLYYLDLQIENSE
                     WPGVADEVLAELGIHEDVLLDFIDVALERFHSVGE"
     assembly_gap    3879..3899
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3900..4085
                     /locus_tag="PLV68_01135"
     CDS             <3900..4085
                     /locus_tag="PLV68_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99868.1"
                     /translation="DAGWGGQTLEHLAFRVLRENFPQLSGEDVHTVVVAAHRVYIQRH
                     PQRSVHLKRPEELRRPV"
     gene            4122..4937
                     /locus_tag="PLV68_01140"
     CDS             4122..4937
                     /locus_tag="PLV68_01140"
                     /inference="COORDINATES: protein motif:HMM:NF025053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HRD99869.1"
                     /translation="MALIIDACFAQHQGDRKEQQDRVAIIPHPKVKGVALAVVADGMG
                     GHTGGSLAAEQVVHTAKTSLEHFGPTEESPQHMLENSMREAHTMIKASRFMNEKDPHS
                     TAVMLLLQPGKVSWAHCGDSRMYRFHGDKLRFRTIDHSYVEHLLATGKITPEQAEHHP
                     HKNVLLTSLGGGEDPKFDFGEASDIADGDSFILCSDGLWAYFSDAELGGVIAAFSARE
                     ASEVLINRARERGAGGGDNCSLAIVKFKEKPADKPKLPPGFKPRPGFPPIDQK"
     gene            complement(5367..5531)
                     /locus_tag="PLV68_01145"
                     /pseudo
     CDS             complement(5367..5531)
                     /locus_tag="PLV68_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017899411.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C-terminal helicase domain-containing protein"
     gene            complement(5950..6353)
                     /locus_tag="PLV68_01150"
                     /pseudo
     CDS             complement(5950..6353)
                     /locus_tag="PLV68_01150"
                     /inference="COORDINATES: protein motif:HMM:NF039815.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7597..8643)
                     /locus_tag="PLV68_01155"
     CDS             complement(7597..8643)
                     /locus_tag="PLV68_01155"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRD99870.1"
                     /translation="MRLPRVLATIAAVSMISLAACGSDDNATTSTSANTSASTTSSSA
                     ASSSTTPSGSDAPTTTASTATTGESVPTSSAPSTDAPTTDPAPIEDKIVVIGEEYLLA
                     DLLALDVVPFASTATVADKGFQGLDAYDTSGIQILPATTLNVEALATMAPDRIIILNF
                     FANEVGEDLLNGIAPTTVIPDGLTPEQLITIYGEMFDRKAQADALIAELDAARADATA
                     ALSGLSVSAATIYSGPSLAAYVSGPWTVPQTLLDAGATLVPDPSQVEPDRNGRGWLSL
                     EQIGLLSAPQLLLFQTETVDGEDAAIEAIEANPLWAQLPAVKSGDVTTFDRLGYPGIE
                     GRVRLIADLIAALA"
     gene            8761..8916
                     /locus_tag="PLV68_01160"
     CDS             8761..8916
                     /locus_tag="PLV68_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99871.1"
                     /translation="MPGAVAIILVLLLLPVVVCMSFAGIAFVFGQLLWKDGEVRNEGS
                     ELIDLNA"
     gene            8985..10328
                     /locus_tag="PLV68_01165"
     CDS             8985..10328
                     /locus_tag="PLV68_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012784166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRD99872.1"
                     /translation="MHVHDATIAALTDQIVAYAVERVRATDLPLDGPATAEALEAAAG
                     QTITAPGIGGEAALALFRDVLAPATISSDHPGFLSFVPAAPTEASVLFDLIVGASSIY
                     GGSWMEGAGAVFAENQALRWIADLVGLPADAGGVFVSGGTAGNLSALIAARHAWRARN
                     RRLARVRGIIVSSTGSHSSIASAARAMDADLVTVPADERGRMSADSLAVVLERLDPDD
                     RERIFAIVTTNAGVIDDLDGVGAIAAERDIWLHVDGAYGGAALCSPAVRPRFAGIEQC
                     DSFIVDPHKWLFAPFDCCALVYRHPEIAREAHTQHAEYLDVLTGEWNPSDYAHHLTRR
                     VRGLPFWFSLATHGTDAYARAIDTTLAVAGEVADMVRTMDHVELVVEPELSVVLFRRH
                     GWQPADYHAWSDRLLAAGTGLVVPTSWAGETVLRFCFVNPLTTPALVSEILESMR"
     gene            10352..>11285
                     /locus_tag="PLV68_01170"
     CDS             10352..>11285
                     /locus_tag="PLV68_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRD99873.1"
                     /translation="MPQQADLLVRNARLVATVDEQRREIPGGWVAITGGLVSAIGAST
                     DAPPTAAQIIDATDCLVTPGLVNTHHHLFQNLTRAYPPMTKAPLFGWLQSLYPLWSAI
                     DQEAVFTSAWVGLAELALSGCTTSTDHLYLHPRGAGDLLAAEIAAAQDIGMRFHPTRG
                     SMSLSVKDGGLPPDNVVQDDDEILAQSQLAVQRYHDRSHGAMVRIALAPCSPFSVTES
                     LMVRSAELAEQLDVRLHTHFAENSEDDEFSLATFGCRPMEYLERTGWCSDRTWVAHCV
                     MPDHDEVHRLGAAGVGAAHCPSSNLILASGIAPVV"
BASE COUNT         1779 a         3546 c         3867 g         2031 t
ORIGIN      
        1 aatcggatgg cacgccggaa gaggtcgccg aagaagtggc gcgcatgcag gcggtgctgg
       61 ccagcagcgg cgccagcgat ctgcgcgtgt cgcagtccga ggccgagcgc ctgcggttct
      121 gggcggggcg caaggccgcc ttccccgcgg tcggacggat tacgccggac tactactgca
      181 tggatggcac cattccccgc aagcgtcttt ccgagatgct gcaggccatt gccgagatgg
      241 agcgcaagta cgggctgcgt tgcgccaatg tcttccacgc cggcgatggc aatctgcatc
      301 cgttgatcat gtacgacgcg gcggtgccgg gtgagctgca tcgcgccgag gaattcggcg
      361 ccgagattct ggagctgtcg gtggcactgg gcggctcgat taccggcgag catggtgtcg
      421 gcatcgagaa gatcaaccag atgtgcgtgc agttcgcgac gcccgagctg gagagcttcc
      481 atcgggtcaa ggcggccttt gacgaagccg ggctgctcaa ccccggcaag gcggtgccga
      541 ccctgcaccg ttgcgccgaa tatggccgca tgcacgtgca tcatggcgaa acaaaattcc
      601 ctgaactgga ccgtttctga tgacacttga cgatctcgtc gcccgggtag ctgcggccgc
      661 cgaagcgggc accgcgctgc gcgttcgcgg cggcggcagc aaggattttt acggcggcat
      721 gctcgccggc gaacttctcg atgtttccgg tcaccgcggc attctggcct atgagccgac
      781 cgagctttat ctgaccgcac gcgccggtac gccgctggcc gaagttgagg cggcgctggc
      841 cgagcgcggc cagatgctgc ccttcgaacc gccgcatttt ggtagcgcca ccgttggcgg
      901 ttgcgtcgcc gctgccctcg ccggcccgcg gcgccagcag gcgggtggcg tacgcgatta
      961 cgtgctgggc gtgcgcatga tcgacggccg cgggcagcta ctgaattttg gcgggcaggt
     1021 catgaagaat gtcgccggat tcgatctcgc ccgcttgctt accggcagcc tggggacgct
     1081 gggggtattg gccgaggtga ctttcaaggt cctgccgcgc ccggtggccg agaccacgct
     1141 gcattttgaa ctgtcccagg ccgaagcgct ggagcaactc aatcgctggg gcgggcaacc
     1201 cttgccgatt tcaggctctt tctggcacga ggggcagttg acggtcaggc tctcgggggc
     1261 gcatgcggcg gtcgattcgg cccgccgaat cctcggtggc gaacacgtgc cgcaggccga
     1321 gggctggtgg cagtcggtgc gcgagcagac ccatgccgcc tatgcggccg atcgcctgtg
     1381 gcggctggcg ctgccgacga cggcgccagt gctggacttg cccggcgaag tagcggtgga
     1441 atggggcggc gggctgcgct ggttgcgttc cgatgccgag gtggccgttg ttcgtcaagc
     1501 cgcggccgag gtcggcggcc atgccaccct gtatcgtgcg ccggaatcgc tgcgctgtct
     1561 ggaaaatgcc ttcacgcccc tgcccctggc cgtactggcc ctccatcaac gcttgaaaaa
     1621 agctttcgac cccaagggga ttttcaaccc cgggcgtctt ttcgccgagg tgtaaggttc
     1681 atgcatagcg aacttgccga tttcatccgc gacacccccg atggccagga ggcggcggcc
     1741 attctcaaga agtgcgtgca ctgcggtttc tgcaccgcga cctgcccgac ctatcaactg
     1801 ctgggcgatg agctcgatgg tccgcgcggt cgcatctatc tgatcaagca ggtactcgaa
     1861 ggcaagccgg ctaccgcgaa gacccagctt catctggatc gctgtctgac ctgtcgctcc
     1921 tgcgaaacga cctgcccgtc cggggtcaag tacagccatt tgctcgatat cgggcgcaag
     1981 gtggtcgacg cgcaggtgcc acgtcgcctg ggggcgagcg tcgtgcgcct ggccctgaag
     2041 acggccttgc cccgtgcatg gatattcaag ccggcggtca agttggggca actgatgcgg
     2101 cccttgttgc ccgcgctgct ggcggagaag atgccggcgg cagtcgccgg cagccgccgg
     2161 ccgtggccgg aagcaaaagc gcacccgcgg cgcatgctga tgctggcggg gtgcgtgcag
     2221 cccgctctgg cgccgagcat caatgtcgcc atggcgcgga tgctggaccg cctcggcatc
     2281 agcccggttg aagcgacggg ggcaggctgt tgcggcgcct tgaagtttca cctgaacgac
     2341 catgcagcgg ccctggacga catgcggcgc aatatcgacg cctggtggcc gcacgtggaa
     2401 gctggtgccg aagccattgt cgtgaccgcg tccggctgcg gggtccaggt caaggattat
     2461 ggccatgcct tgcgtcacga tgccgaatac nnnnnnnnnn aaggctgagc atatcagcaa
     2521 gctgtgccgc gacccttcag agattctggc ggcagaaaag gagcgattgc tgcccttgtt
     2581 gaccggggtc aagccggcgc gcgaaaaatt ggcgtttcat tctccttgta ccttgcaaca
     2641 tggcctgcag attcgtggtg cagtggaaga ggtgctgggc gccgccggtt tttcactgac
     2701 accagtcaag gatgcccacc tgtgctgcgg ttcggccgga acctactcag tactgcagcc
     2761 ggatttgtcg catcagctgc gtgacaacaa gctggcggcg ctgggcgccg gcgggccgga
     2821 gcggatcgcc accgccaata tcggttgcct gacgcatctg caagccggca gcacgacgcc
     2881 cgtggtgcat tggatcgaat tgatagacga gagcttgccg tgacactgac accggttgcc
     2941 gacattgtaa aaacactgga tgcgcctgat gcggcgatgg tcaagcggct gctcgaacgc
     3001 ggcatgatga ttccggtgca gccgcgggtg ctggaagaat tgcggcagca gatgctgaaa
     3061 aaggaattcg atatccgcgt gctgtcgcgc atcatcaact ccgaccccgg tattgtcgcc
     3121 atgctgttca aggcctgcgg caattcggcc tatcgccagc atcagccctt ccagtcggtg
     3181 gacgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnaacct gatccaggcc atcgccgttg
     3241 ccagcctgca cgacagcaag cagaacagcg aagcctacga gtcgttctgg gtgcgttcgc
     3301 aggcggtggc acagctggcg atgatcgttg ccgatgaacg catttcggtg tgcaacctct
     3361 tcccggatca ggcctatctt gccggcatgt tccacgattg cggggtggca ctgctgatgc
     3421 ggcgcttctc gacctattgc aaggagatga agctgggtga accggggcgc tggatcgatc
     3481 tcgccgagga agacgccaag ttcaaggcgg atcattgcgt cgtcggatat ctgattgccc
     3541 ggcactggaa cttgccggac ttcatctgcg acgctatccg caaccaccac gacatccagc
     3601 gcatcggcag cagtcacgcg tcgcgcagta tggtcgcgat tctgcagctg gcgatccatc
     3661 tctactatct tgatctgcag atcgagaatt cggagtggcc cggcgttgcc gacgaggtgc
     3721 ttgcggaatt aggtattcac gaggatgtcc tgctggattt cattgacgtt gcactggagc
     3781 gctttcacag cgtcggggag tgatgatgac tacaccggaa gccatggcag agcgaaggcg
     3841 caacccggcg ctgcgcgacc tgttcgatac ggcctatgnn nnnnnnnnnn nnnnnnnnng
     3901 atgccggctg gggtgggcag acactggaac atctcgcttt tcgcgtcctg cgcgagaact
     3961 ttccgcagtt gtccggcgag gatgtgcaca cggtcgtggt tgctgcccat cgcgtttata
     4021 tccagcgcca tccacagcgc agcgtgcatc tgaaacggcc ggaggagctg cgtcgtcctg
     4081 tatgactttc gtgataagct gccccgcaac aggaggagag catggcacta atcattgatg
     4141 cctgttttgc gcagcatcag ggggatcgca aggaacagca ggatcgcgtt gccatcattc
     4201 cccaccccaa ggtcaagggc gtagcgctgg cggtggtggc ggatggcatg ggcggtcata
     4261 ccggcggttc gctggccgcg gaacaggtgg tgcataccgc caagacgagt ctggaacatt
     4321 tcggaccgac ggaagaatcg ccgcagcaca tgctggaaaa cagcatgcgc gaagcccaca
     4381 cgatgatcaa ggcctcgcgc ttcatgaacg aaaaggatcc gcacagcact gccgtcatgc
     4441 tgctgctgca gccgggcaag gtgagctggg cacattgcgg tgacagccgc atgtatcgct
     4501 ttcacggcga caagctgcgc tttcgcacca tcgatcactc ctacgttgaa cacttgctgg
     4561 ctaccggcaa gatcactccc gagcaggccg agcatcatcc ccacaagaac gtattactga
     4621 cttccctggg cggcggtgaa gaccccaaat tcgatttcgg tgaggccagc gatattgccg
     4681 atggcgacag tttcatcctc tgttccgatg gtttgtgggc ctatttcagc gatgccgaac
     4741 tggggggggt gatcgccgcc ttttctgcgc gtgaggcgtc cgaggtgctg atcaatcgtg
     4801 cccgcgaacg cggcgccggc ggcggcgaca attgctcgct ggcgatcgtg aaattcaagg
     4861 aaaaaccggc cgacaaaccc aagttgccgc caggtttcaa gccgcggcca ggatttccgc
     4921 cgatcgatca gaaatgaggg ctggggcggt cagtacgcag gcgggctgca acgcgggtta
     4981 aaccagaacg ccatggtgcg tctggtgtag tgctccgctt acttgcgtcc gagcccgccc
     5041 agcaacgctg ccaccacctt gcgcggtttg tgcggttccg ccttgaccgg cgccttgggt
     5101 gaatccggca agtcgctgcg cggcgtggca tcgtcgtagg gcttgctgaa gtcgaagcca
     5161 tcggcggcaa tcatcgggtt cttccgcgcc gggcgctggg ctacgggggc cttcggcggc
     5221 gctgcagcag ccgagcgccg gcgccggttg ccgggcgggt attcgtagtg gtcttccggc
     5281 tcgaagccgg cgatctcgat ctgctcgatc gggcgcttga tcaatttttc gatgtcgacc
     5341 agatactgca cctcggcggc gctgaccaag gaaatggcgt tgcccagctt gccggcccgg
     5401 ccggtgcgtc cgatccgatg cacgtagtct tccggcgtgt gcgggagttc ataattcacg
     5461 acgtgcggca gatcgtcgat gtcgaggcca cgggccgcga catccgtggc gacaaggacg
     5521 atgacctcgc ccgacttctc gcacgtgacc ttcgcggtcg tcagcggtcg gaccgacatg
     5581 gtcgatgccc cgccggcagc gagtggacgg ccggtggtcg ggcggtacgc gggcagggtc
     5641 ggttcggttg cttcaccgtc tgcgttggct tcggtctcgc cggccggttc gccagtgggt
     5701 tcgccagccg gggcatcgtc ggcgatcttc gcggcttccc actcggcgcg gcgctgctcg
     5761 accccgacgc gccagtccgg ttcgtagcag tccaagcccg cgtcggacgg gtcgagtggg
     5821 ttgcggccct cggtgtggtt ctcgccgtgg tcccagcagc cgtccccgtc ggtatcgggg
     5881 ttgagcggat cggtgccgta gtacgtgatc tcctcgtccc acagcaggtc gaagtcgctg
     5941 tcgatgttga gcggatcggt gccgtagacg ttgacctctt cgccatcggt cagccggtcg
     6001 ccgtcggtgt cggcgttgaa cgggtcggtg cccaacagaa cttcgaggga atcggtgagg
     6061 ccgtccccgt cggagtcggt gtcgccgtcg caggcggacg gatcgtcgat gggttggccg
     6121 gtcgccagat cgaagcagtc tgcctggccg cggtcggtga cctcgaggaa gctgagcacc
     6181 gcgccgagcg gcacgtcggt gttgacgatc gcccgggtgg tgccgtcgtc ggcgacgtca
     6241 cacgcgtcga tcagtccgta gaagccgtcg tcgcccgcga tcgcggtcga ggcaaggaat
     6301 ggcgctccca gcacgcaccc gccgttcaac tgggcttcgt cgatgccagg catctcgctc
     6361 gcgctggtac gagtggtgtt ggctggatcg agcacgatct gcactgccga ctcgccgacc
     6421 acttcggcct gcaacgcagc gaagtcgttg ggcaccaccg tgttggcggc caacgcgaag
     6481 ggcgattcgt gtccgtccca gctcgcggtg gtgaccgagg tcgacccgta cggaacggtg
     6541 tcgcccgcgc cgagcgcgaa gacggtgaag gtgtacgtcg atgtcggcgc cagatcgacg
     6601 acggccacct cggtggccag cggatcggcg agcgcgacct cgacgccttc ttcgggggat
     6661 tgcgctggcg tcgcgccgtc ggtgcggcgt acctggaact gggccacgcc atcgggaccc
     6721 ttccatgcca acgtcgcgcc gatcgcgctc acccgcttga cggacacgtg cacggcggcg
     6781 acgtggatgt cgaccccgaa gtcgaccgcc gcgatctgct cgggtgcttc tgcggtgggg
     6841 atgccgaccg tgacctcggc ggtcacgtcg cgggccacga aggtcgctcc gtacccgatc
     6901 gatggcgcgt cggcggtgat ccggtacgtg ccgggctcga cgtcgaggaa cgccgcggtg
     6961 tcgacaccgg ccccgtcgac ggcagcggcc accgagacgc cggtgtcgac gttggtgacc
     7021 gtgaacaccg gacgggtctg cggtccggcg ctgccgtccg ggttcttgaa cacgatcgcc
     7081 gaacgaccgc ccgtgaacgg ctctggttcg gtggtcgtag tggccaccac ggtgtcgggg
     7141 gtcgtcgtcg tggccaccgt gtcgggggcg gcctcggtcg tggtgggcgc cgagctcggc
     7201 accgactcgg agggcttgga cgaacaggcc gcggccacgg tgagaccggc aaccgtcacc
     7261 ccgatccaga tgcgccggtt cccccggcgc tcggggcgcg ttgcagtatc gctcacgcgt
     7321 gagatccctg gtttcactat tgctcctgtc cgtggagagg cggtggcgcg cgtcagcccg
     7381 cacccttccc ggtccgcctc gcccgagcac catagaggcg agcggttacc gagacctaaa
     7441 tggatccacc gcaacccgaa ggacagatgc gtcggcgaca atggcacggt cgcgagcggc
     7501 cgatcgtgag tgacggaccc atcagcgagc agcgacggcg aaggtgtctt gctcgcccgc
     7561 acctgagcgg ggcgatcagg ctggcgacgg gtcggctcag gccagggcgg cgatgaggtc
     7621 ggcgatcagg cgaacccggc cttcgatgcc cggatacccg aggcggtcga aggtggtcac
     7681 gtcaccgctc ttgaccgcag gcagctgggc ccacagcgga ttggcctcga tcgcctcgat
     7741 cgccgcgtct tcaccgtcga ccgtctcggt ctggaacagc agcagttgcg gtgcgctgag
     7801 cagaccgatc tgctccagtg agagccagcc gcggccgttg cggtcgggct cgacctggct
     7861 ggggtcgggc accagcgtgg cgcctgcgtc gagcaatgtc tgcggcacgg tccacggtcc
     7921 gctcacgtag gcagccaggc tcggcccgga gtagatcgtg gccgcagaga ccgataggcc
     7981 cgacagcgct gccgtcgcgt cggcgcgggc ggcgtcgagc tcggcgatca gcgcgtcggc
     8041 ctgggccttg cgatcgaaca tctcgccgta gatcgtgatc agttgttcgg gggtcagccc
     8101 gtcggggatc accgtggtcg gcgcgatgcc gttgagcagg tcttcgccga cctcgttggc
     8161 gaagaagttg aggatgatga ttcgatccgg agccatggtg gccagcgctt ccacgttgag
     8221 cgtggtagcg ggcaggatct ggatgccgct ggtgtcgtag gcgtcgagtc cctggaaccc
     8281 cttgtcggcc acggtcgcgg tcgacgcgaa cggcacgacg tcgagcgcga gcaggtcggc
     8341 cagcaggtac tcctcgccga tcacgacgat cttgtcctcg atcggagcag ggtcggtggt
     8401 gggcgcgtcg gtggacggtg cgctgctggt cgggaccgat tcgcccgtgg tggctgtcga
     8461 ggcggtggtc gtgggggcat cgctgccgga cggcgtcgtg ctggacgacg cggcgctcga
     8521 cgaggtcgtg ctggccgagg tgttggccga ggtgctggtc gttgcgttgt cgtcgctccc
     8581 gcaggcggca agcgagatca tggacacggc ggcgatcgtg gcgaggactc gggggaggcg
     8641 catgagaagc gagcctaacg ttgtctgctt gccacggtga cgcgtgggac gacgtggcgg
     8701 tggggatgca acgacgtcct gcttggtcgg tggcagcgcc ggctgggtac agtggatccc
     8761 gtgcctggtg ctgtcgccat catcctcgta ctgttgctgc tgcccgtcgt ggtctgcatg
     8821 tcgtttgcgg gcatcgcgtt cgtcttcggg cagttgttgt ggaaagacgg cgaggtgcgc
     8881 aacgagggca gcgaactgat cgatctcaac gcctgatcgc ggcatccgaa cgccgccgca
     8941 cgcggcggag ctggtgactt ttcctgcgtg ggtaacctcg ggctgtgcac gtccacgacg
     9001 cgaccatcgc agcattgacc gaccagatcg tcgcgtacgc ggtcgagcga gtgcgtgcga
     9061 cggacctccc gctcgacggt cccgcgacgg ccgaggcgct cgaggcggcc gcgggacaga
     9121 cgatcactgc gccaggcatc ggtggcgagg ccgcgctggc gctgttccgc gacgtgctcg
     9181 ccccggccac catcagcagt gaccatcccg gcttcttgtc gttcgtgccc gctgccccga
     9241 ccgaggcgtc ggtgctgttc gatctgatcg tcggggccag ctcgatctac ggcggctcgt
     9301 ggatggaggg cgcgggggct gtgttcgccg agaaccaggc gttgcggtgg atcgccgatc
     9361 tggtcggtct gcccgccgac gccggcggcg tgttcgtgag cggcggcacc gctggcaacc
     9421 tgtccgcgct gatcgcggcc cgccacgcat ggcgtgctcg caaccggcgc ttggcccgcg
     9481 tccggggcat catcgtcagt tcgaccggat cgcacagctc catcgcgtcg gccgcgcggg
     9541 cgatggacgc cgacctcgtc acggtgcccg ccgacgagcg gggacggatg tcggccgaca
     9601 gcctcgccgt ggtcctcgaa cggctcgatc ccgacgaccg ggaacgcatc ttcgcgatcg
     9661 tcacgaccaa cgccggcgtc atcgacgacc tggatggggt cggagcgatc gctgccgagc
     9721 gagacatctg gctgcacgtc gacggcgcct acggcggtgc ggcactgtgt tcgcccgcgg
     9781 tccggccacg ctttgccggc atcgagcagt gcgacagctt catcgtcgat ccgcacaagt
     9841 ggctgttcgc gccgttcgac tgctgcgcgc tggtgtaccg ccatcccgag atcgcccgcg
     9901 aggcgcacac gcaacacgcc gaatatctcg acgtgctgac cggcgagtgg aaccccagcg
     9961 actacgccca ccacctgacc cggcgggtgc gcggcctgcc gttctggttc agcctggcca
    10021 cccacggcac cgatgcctat gcccgggcaa tcgacacgac cctcgccgtt gctggcgaag
    10081 ttgccgacat ggtgcggacg atggaccatg tggaactcgt cgtcgaaccc gagctgtcgg
    10141 tggtgctgtt ccgccgtcac ggctggcagc ccgccgacta ccacgcctgg agcgaccgcc
    10201 tgctggccgc cggcacgggg ctggtcgtgc ccacttcgtg ggccggcgag accgtgctgc
    10261 gcttctgctt cgtcaacccg ctgaccaccc ccgcgctggt cagcgagatc ttggagagta
    10321 tgcgctgatg tcgacgcaac catcacaacc tgtgccgcag caagccgatc tgctggtccg
    10381 caacgcgcgg ctggtcgcca ccgtggacga gcagcgccgt gagattcccg gcggctgggt
    10441 ggcgatcacc ggtgggttgg tctcggccat cggcgcctcg accgatgcgc cgcccaccgc
    10501 ggcgcagatc atcgacgcca ccgactgcct cgtcacgccc gggctggtca acacccacca
    10561 ccacctgttc cagaacctca ctcgggccta cccgccgatg accaaggcgc cgctgttcgg
    10621 gtggttgcag tcgctgtatc cgctgtggtc ggcgatcgac caggaggccg tgttcacgtc
    10681 ggcatgggtg ggtctggccg agttggcgct cagcggatgc acgacatcga ccgaccacct
    10741 gtacctgcat ccacgcggcg cgggcgacct gctcgcggcg gagatcgccg cggcgcagga
    10801 catcggcatg cgctttcacc cgactcgcgg gtcgatgtcg ctgtcggtca aggatggagg
    10861 gctgccgccc gacaacgtgg tccaggacga cgacgagatc ctggcccaga gccagttggc
    10921 cgtgcagcgt taccacgatc ggtcacacgg cgcgatggtg cgcatcgcgc tcgcaccgtg
    10981 ctcgccgttc tcggtcaccg agtcgttgat ggtccgttcg gccgaactgg ccgaacagct
    11041 cgacgtgcgg ctgcacaccc acttcgccga gaacagcgag gacgacgaat tctcgttggc
    11101 cacgttcggc tgtcgcccga tggagtactt ggagcgcacc ggctggtgct cggaccgcac
    11161 gtgggtggcg cactgcgtga tgccggacca cgacgaggtg caccgcttgg gtgccgccgg
    11221 ggtcggtgcc gcacactgcc cgagcagcaa cctgatcctg gcctcgggca tcgccccggt
    11281 ggtcg
//
LOCUS       DAORNF010000014        10838 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_4547_length_10838_cov_1.666883, whole genome shotgun sequence.
ACCESSION   DAORNF010000014 DAORNF010000000
VERSION     DAORNF010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 10838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10838
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4547_length_10838_cov_1.666883"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..553)
                     /locus_tag="PLV68_01175"
     CDS             complement(<1..553)
                     /locus_tag="PLV68_01175"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRD99874.1"
                     /translation="MADTHDPAPQTDTDSTEIDAVDEECPVVLVHGWGGSFRETWERS
                     GFTALLADAGRSVIGVDLLGHGTAPKPHDPAAYGDLTERIRDALPEAGQVDAIGFSMG
                     AMTLLRLAIDQPHRFRRLVLAGIGQNVFVHDAERHAAIVAALEGVDGADTDNVGRLFV
                     QYASQPGNDIAALTAVLRRAESAP"
     gene            606..>1946
                     /locus_tag="PLV68_01180"
     CDS             606..>1946
                     /locus_tag="PLV68_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GuaB1 family IMP dehydrogenase-related protein"
                     /protein_id="HRD99875.1"
                     /translation="MRFLHDAPPYDLTYSDVFMVPSLSSVPSRFDVDLATPDGLGTTL
                     PIVVANMTAVAGRRMAETVARRGGLTVLPQDIPLDVIESVVQWVKSRHPVYETPITLS
                     PHHTVGDALGLIHKRAHGAVIVIDDLQRPVGIFTEHDAAGFDRFTQLQNTMSQELITL
                     DASVSAPEIYDRLSDQRISMAPVLDDDGRLLGCVTRKGALRSTIYRPALDAQGRLMIA
                     VAVGINGDPAQRAKALVEMGVDVLVIDTAHGHQTRTLRAIEEVRAVAASTPIVAGNVV
                     TPEGTRALIDAGADIVKVGVGPGAMCTTRMMTGVGRPQFSAVLECSAEAAGHGKHVWA
                     DGGVRYPRDVALALAAGGSSVMIGSWFAGTYESAADTLRSTDGRLYKESFGMASNRAV
                     KNRNRFESPFERAAKELFEEGISTSRMYLDHDRPGVEDILDQIVAGVRSACTYAG"
     assembly_gap    1947..1962
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2023..2715
                     /gene="cmk"
                     /locus_tag="PLV68_01185"
     CDS             2023..2715
                     /gene="cmk"
                     /locus_tag="PLV68_01185"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HRD99876.1"
                     /translation="MSGELNRLRVVAIDGPAGAGKSTVARALARRLGLRYLDTGAMYR
                     AVTAAALDRDIDLADAAAIARLARTIDIDVTDAAVTVDGADVTVAIRTAAVTAAVSAL
                     AANSGVRDELRRRQREWAVANGGGVIEGRDIGTVVFPDAVLKLYLTASPRVRAQRRVG
                     EAGGDVDEMERTIAARDHSDANRTDGPLREGTDAVVIDTSALTIAEVVSMLAALVAER
                     EHGVMHAPDGDR"
     gene            2712..>3025
                     /locus_tag="PLV68_01190"
     CDS             2712..>3025
                     /locus_tag="PLV68_01190"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRD99877.1"
                     /translation="MSEVDSYLAKQSLLSKANYQLWKFVIAGTTRVYTRMRIEGREHI
                     PASGGFVLAPVHRSYIDTPIASLVTRRRLRFMAKDTMWKHRLGGWLISSVGGFPVTRG
                     SS"
     assembly_gap    3026..3035
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3036..3400
                     /locus_tag="PLV68_01195"
     CDS             <3036..3400
                     /locus_tag="PLV68_01195"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRD99878.1"
                     /translation="FHLLVQGEPIVMFPEGERKSGPIVQPLFEGAAYVAAKGDVPIIP
                     VGIGGSEQVMPKKAKFMYPHKVVVIIGAPIVPAKNEQGRTPRSELKQITAQLHDELQR
                     LFDAAQIKAGSPNPPRAD"
     assembly_gap    3527..3590
                     /estimated_length=64
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3591..>4585)
                     /locus_tag="PLV68_01200"
     CDS             complement(<3591..>4585)
                     /locus_tag="PLV68_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HRD99879.1"
                     /translation="AESDNWAALGAGLDAGYVVRGGALVAWRRPLGVAPTTPFHIVGA
                     HTDSPCLRVKPNPDTGAFGWRQLAVEVYGGVLANSWLDRDLGVAGRVVLADGTHRDVA
                     VHEPIARVPQLAIHLDREVNERGLQLDKQQHLAPVWGTGTANTGDFAAWLATRPELDG
                     CSPAEVAWWDLCLFDVQGASVLGSDASLLASGRLDNQLSCWAATSALAGVGGQDGPVG
                     HTAVIALFDHEEVGSESTTGAAGPLLATVLERLSTAAGATRDQFQRALVASACVSADN
                     AHAVHPNYPERHEPGHRPIVNAGPAIKINANQRYATDAATAVLFQQACDQAGVPW"
     assembly_gap    4586..4621
                     /estimated_length=36
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4749..5411)
                     /locus_tag="PLV68_01205"
     CDS             complement(4749..5411)
                     /locus_tag="PLV68_01205"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRD99880.1"
                     /translation="MRATIAGGVVKGCDVVELIMVRHALPVRRELLSGAADPEVSEAG
                     RAQAELLGDYLATEELQAIYASPMLRARQTASPLALRVGLDVTIIDGVAEFDQHASEY
                     IPVEELRATNDPRWQQMVNGDWLDGPESRDAFITRVIESLDALVRTHPGIKIAVVCHG
                     GVINAYLAHVLGLAPGSQFFYPNYTSIHRVVASSRGHRQIVSVNESFHLRGSGLPIGL
                     YS"
     gene            5445..>6752
                     /gene="pyk"
                     /locus_tag="PLV68_01210"
     CDS             5445..>6752
                     /gene="pyk"
                     /locus_tag="PLV68_01210"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004491.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HRD99881.1"
                     /translation="MTGRTKIVATIGPASQAPETLRAMIAAGVDVVRLNLSHGELSEH
                     IERLHAVRAAAADVGRVVAVLADLPGPKVRAASFPDGGVLLRVGNLITLAPGDQRSDG
                     DRIEVDYPTLLADIAPGDRMVIGDGGIVLRVASVDVGEAHAVVESGGLVQGRPGVHLP
                     SERLRLRTPTEHDLTLACAMAEEGVDFLGVSFVRAAADLDAVRAAIATRLVAKFETTP
                     AINDMEAIVGNADAVMVARGDLGIECSLEDVPHLQKRIIRHCVEIGVPVITATQMLES
                     MIGAPAPTRAEVSDVANAVFDGTDALMLSGETAVGADPVNVVKTMARVAERAEASANY
                     ARWSERLGRVQQRWAHRVDPITAAITHACAQAANDAGAEAIVCCTRSGRTAAAMSRFR
                     PPALLIGASPDPATVRAMALTWGVVPIQVGSYGSTDELVWFAIE"
     assembly_gap    6753..6762
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6871..7695
                     /locus_tag="PLV68_01215"
     CDS             6871..7695
                     /locus_tag="PLV68_01215"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRD99882.1"
                     /translation="MGCGSQPSGGTAPQIGQIWADEAGPEDAPLIALVHGSMDRSTGL
                     LRLSRRLDDRWRVLRYDRRGYGRSLPHDGPYSMDHQVADLVALLAGRPAVILGHSYGG
                     NVALATAARHPELVAALALYETPLSWEPWWPGATVGSTALAAPGGPPEAAERFMRSFI
                     GDDAWEALPDKTRVTRRREGIAMVCELGDVRDTPPWHESAIACPVVLGYGANGAAHHA
                     AGMRHLCELLTGTTLVEVPDADHRAPHDRPTAIAEMLVDPAIRAAGQPWTDALSLA"
     gene            complement(7682..7993)
                     /locus_tag="PLV68_01220"
     CDS             complement(7682..7993)
                     /locus_tag="PLV68_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99883.1"
                     /translation="MGLPVQSMDVMVALTLASAKWIGLAIIAALLVMAGFLAKMISGF
                     SRKMVAIALCSALAFAVYTQRAALQRCADSVAEIPDGRTAVCQFFGQEITIKAPLVTP
                     D"
     gene            8034..8468
                     /locus_tag="PLV68_01225"
     CDS             8034..8468
                     /locus_tag="PLV68_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3151 family protein"
                     /protein_id="HRD99884.1"
                     /translation="MSDERGVQFRGSGLPSTVLPPIDQATADALAAARASGEPRDAIA
                     AVVARVPRCLDAWADLGDAGRDVVERYSAYRVGYHRGLDALRANGWRGSGYVRWAEAS
                     NRGFLRSLRGLGAMAAEIGEHDEAERIAVFLRQLDPSGIPAA"
     gene            8474..9079
                     /locus_tag="PLV68_01230"
     CDS             8474..9079
                     /locus_tag="PLV68_01230"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HRD99885.1"
                     /translation="MPGAALGAVFVGGQSRRMGTDKALIEAGGERLVDRTARILREAG
                     CEQVAAIGPRHLAGDYVGSVGLVADDHPGAGPLGALLTALRLAAQAGAAGVMVAACDL
                     PALDVATVAAVLAAASPGNGHVGIAVADSGRREPLLSWWHTRTLATVEAAFDAGERSV
                     LRVIEHVAMGPAMGPAVGAVVDVPVGADAVFNMNTPDQRPE"
     gene            9094..9480
                     /locus_tag="PLV68_01235"
     CDS             9094..9480
                     /locus_tag="PLV68_01235"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRD99886.1"
                     /translation="MAVQEVSLEAFAAALEAGLVGRVVDVREPGEYAAGHVPGAVLVP
                     LANVPDSIDAFAGDGPTYVVCQVGGPASTWPTRMSPRSTSPEAQRHGSPAVAPWLPAT
                     NPETSDLCQGRAATTTGSTTRSVSIS"
     gene            9420..10583
                     /locus_tag="PLV68_01240"
     CDS             9420..10583
                     /locus_tag="PLV68_01240"
                     /inference="COORDINATES: protein motif:HMM:NF013755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HRD99887.1"
                     /translation="MPGAGGDYDWIDDQVGLDQLIDTLLGQPRYAFDTEFHRERTYFP
                     RLALAQFAWDGGIALVDPLAVDVTALQRLLHSDVVAVLHAAQQDLDVLQHACGAVPRR
                     LFDTQLASGFTGYGTPSLSSLVHGELHIAVPKGDRLTDWLRRPLTADQRSYAASDVAY
                     LLELTDRLNERLERDGRFAWAEAACEELRTRPVGGADPDSAWLRIKDVRTLKPRARGV
                     ARSVAAWRERRAMAVDTPVRHILPDLAILGIAQRQPRTVEDLAHARGVDERHWRGALG
                     KEIIAAVAEGAGHELQMPIGDSDELDRHLRPAVTLVLAWVSELARQEHIDTALLATKN
                     DIVALLRRDPDSRLASGWRAEMLGDNVTRLVEGRAGLTFDGRGGLRLIDVPAS"
     gene            complement(10601..>10838)
                     /locus_tag="PLV68_01245"
     CDS             complement(10601..>10838)
                     /locus_tag="PLV68_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99888.1"
                     /translation="GVIVNKVPGVSSEADRRYDELVRIVGRKAIWQPTIPQRVIVNQA
                     TAERRSIHSYGARAADVSSVFDQLWKRLRRQLKD"
BASE COUNT         1596 a         3636 c         3826 g         1644 t
ORIGIN      
        1 acggcgccga ctcggcacgg cgcaacactg ccgtcagggc ggcgatgtcg ttgcccggct
       61 ggctcgcgta ctgcacgaac aggcgaccga cgttgtcggt gtcggcgccg tcgacgccct
      121 cgagtgcggc gacgatcgcg gcgtggcgct cggcgtcgtg gacgaacacg ttctggccga
      181 tgccggccag caccagtcgg cggaagcgat ggggctggtc gatcgccagg cgcaacagcg
      241 tcatcgcgcc catcgagaac ccgatcgcgt cgacctgacc ggcctcgggc agcgcgtcgc
      301 ggatgcgttc ggtcagatcg ccgtacgcgg cggggtcgtg tggcttcggc gcggtgccgt
      361 ggccgagcag gtcgaccccg atcacgctgc gtccggcatc ggcgagcagg gcggtgaacc
      421 cgctgcgctc ccaggtctcg cggaacgagc caccccaacc gtgcaccaag acgaccggac
      481 attcctcgtc aaccgcatcg atttccgtcg agtccgtgtc cgtctgtggg gctgggtcat
      541 gggtgtcggc catacccggc gacactagag cgtcgggaca gtttcaggac gtagcctggg
      601 gttcgatgcg ctttcttcac gacgccccgc cgtacgacct gacctacagc gacgtcttta
      661 tggtgcccag cctgtccagc gtgcccagcc gcttcgacgt cgatctggcc accccggacg
      721 gcctcggcac gacgctgccg atcgtggtcg ccaacatgac cgcggtcgcc gggcggcgca
      781 tggccgagac cgtcgcccgc cgcggcgggc tcaccgtgct gccccaggac atccccctcg
      841 acgtgatcga gtcggtcgtg cagtgggtca agtcgcgcca cccggtctac gagacaccga
      901 tcacgctcag cccccatcac accgtgggtg acgcgctcgg cctgatccac aagcgcgccc
      961 acggcgcggt gatcgtgatc gacgacctgc agcgtcctgt cggcatcttc accgagcacg
     1021 acgcggccgg cttcgaccgt ttcacccagt tgcagaacac gatgagtcaa gagctgatca
     1081 ccctcgacgc gtcggtgagc gcgcccgaga tctacgaccg gctgtccgac cagcgcatct
     1141 cgatggcgcc cgtgctcgac gacgacggcc gcctgctcgg ctgcgtcacc cgcaaggggg
     1201 ctctgcgtag cacgatctat cgccccgcgc tcgatgcgca gggccgattg atgatcgcgg
     1261 tcgcggtcgg catcaacggc gacccggcac agcgagccaa ggcactcgtc gagatgggcg
     1321 tcgacgtgtt ggtgatcgat acggcccacg gccatcagac ccgcacgctg cgcgcgatcg
     1381 aagaggtccg cgctgtcgct gcctccacgc cgatcgtggc gggcaacgtg gtcacccccg
     1441 aaggcacccg cgcgctgatc gacgccggtg ccgacatcgt caaggtcggc gtcggcccgg
     1501 gggcgatgtg caccacgcgg atgatgaccg gggtcggccg cccgcagttc tccgccgtgc
     1561 tcgagtgctc ggccgaggcg gccggtcacg gcaagcacgt gtgggccgac ggcggtgtgc
     1621 gctatccgcg cgacgtcgcg ctggccctgg cggcgggcgg ttccagcgtg atgatcggct
     1681 cgtggttcgc tggcacctac gagtcggccg ccgacacgct gcgctcgacc gatggtcggc
     1741 tgtacaagga gagcttcggc atggcgtcga accgggcggt caagaaccgc aaccgcttcg
     1801 agtcgccgtt cgaacgagcc gccaaggagc tgttcgaaga gggcatcagc acgtcgcgga
     1861 tgtacctcga ccacgaccgt cccggtgtcg aggacatcct cgaccagatc gtcgccgggg
     1921 tccgctcggc ctgcacctac gcgggtnnnn nnnnnnnnnn nncgtcgtcg gcgtgcagag
     1981 caccgccggg tacgaagagg gccgcccgca gggcaccagc tggtgagcgg cgaattgaac
     2041 agactgcgcg tcgtcgccat cgacgggcct gctggcgcgg gcaaatcgac cgtcgcccgg
     2101 gcgctggctc gccggctcgg cctgcgctac ctcgacaccg gtgcgatgta tcgcgccgtc
     2161 acggcggcgg cactcgaccg cgacatcgac ctggccgacg ccgcggcgat cgcgcggctg
     2221 gcgcgaacga tcgacatcga cgtcaccgac gcggccgtca ccgtcgacgg cgccgacgtc
     2281 accgtcgcga tccgcaccgc ggcggtcacc gcggctgtca gtgcgctggc ggccaacagt
     2341 ggcgtgcgtg acgaactgcg gcggcggcaa cgggagtggg ccgtggccaa cggtggcggc
     2401 gtgatcgaag gccgggacat cggcaccgtc gtgttccccg acgcggtgtt gaagctgtac
     2461 ctcaccgcgt cgccgcgtgt gcgcgcccaa cgccgcgtcg gcgaagctgg cggcgacgtc
     2521 gacgagatgg aacggacgat cgccgcccgc gaccacagcg atgccaaccg caccgacggc
     2581 cccctgcgcg aagggaccga tgcggtggtg atcgacacct cggcgctgac gattgccgag
     2641 gtcgtctcga tgctcgccgc cctggtcgcg gagcgcgaac atggggtgat gcacgcaccg
     2701 gacggtgacc gatgagcgag gtcgactcgt atctcgccaa gcaaagcctg ctcagcaagg
     2761 ccaactacca actgtggaag ttcgtcatcg ccgggacgac ccgggtctac acccggatgc
     2821 gcatcgaggg ccgcgagcac atccccgcca gtggcggatt cgtgctcgcg cccgtgcatc
     2881 gcagctacat cgacaccccg atcgcgtcgc tggtcacccg ccgccggctg cggttcatgg
     2941 ccaaggacac gatgtggaag catcgcctcg gcgggtggct gatctcctcg gtcggtggct
     3001 ttccggtcac ccgcggctcc agcgannnnn nnnnngcttc cacctgctcg tccagggtga
     3061 gccgatcgtg atgttccccg aaggcgagcg caagtcaggc ccgatcgtgc agccgttgtt
     3121 cgaaggcgcg gcctacgtgg ccgccaaagg cgacgtgccg atcatccccg tcggcatcgg
     3181 cggatccgag caggtcatgc cgaagaaggc caagttcatg tacccccaca aggtggtggt
     3241 catcatcggc gcgccgatcg ttcccgccaa gaacgagcag ggccgcacac cgcgcagcga
     3301 actgaagcag atcaccgccc aacttcacga cgagctgcag cgattgttcg atgcagccca
     3361 gatcaaggcc ggaagcccca acccaccgcg cgccgactag cgcaggaacg tgcccagggc
     3421 gcgggcgaga aagcccgggt cgtcggtgcc gcacaactcg cgcgcactgt gcatcgacag
     3481 ttggggaacg ccgacgtcga cggtggggat gcccaggcgt gtcgccnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ccacggcacg
     3601 cccgcctggt cacaggcctg ctggaacagc accgccgtcg ccgcatcggt ggcgtagcgc
     3661 tggttggcgt tgatcttgat cgccgggccg gcgttgacga tcgggcggtg accgggttcg
     3721 tggcgttcgg ggtagttggg gtgcaccgcg tgggcgttgt cggccgacac gcatgcggat
     3781 gcgacgaggg cgcgctggaa ctgatcgcgc gtcgcgcccg ccgccgttga caaccgctcc
     3841 agcaccgtcg ccaacagtgg tccggccgcg ccggtggtgc tctccgagcc gacctcttcg
     3901 tggtcgaaca gcgcgatcac ggcggtgtgg ccgacgggtc catcctgccc accgacgccc
     3961 gccaaggcgc tggtggcggc ccagcagctg agctgattgt ccaatcggcc gctggccagc
     4021 aggctcgcgt cggagccgag cacgctcgcc ccctgcacgt cgaacaggca caggtcccac
     4081 caggccacct cggcggggga gcagccatcc agctctggac gagtagcgag ccaagctgcg
     4141 aagtcgcccg tgttcgcggt gccggtgccc cacaccggtg ccaggtgctg ctgcttgtcg
     4201 agctgcaggc cacgctcgtt gacctctcga tcgaggtgga tcgccaactg cggcacgcgg
     4261 gcgatcggtt cgtggaccgc tacgtcccgg tgggtgccgt cggccagcac gacccggccg
     4321 gcgacgccga ggtcgcggtc gagccacgag ttggcgagca ccccgccgta gacctcgacg
     4381 gcgagttggc gccacccgaa cgcgccggtg tcggggttgg gcttgacccg caggcatggc
     4441 gagtcggtgt gcgcgccgac gatatggaac ggagtcgtgg gcgccacgcc gaggggtcgt
     4501 cgccacgcca ccagggctcc gccgcgcacg acgtatccgg cgtcgaggcc agcacccagg
     4561 gcggcccagt tgtcgctctc ggcaannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4621 ngctgggtgg cggatgcgac cgcatgccag ggggacggcg atgcatcgag gaaggcacag
     4681 agatcggaca gctccacgcc ggtcaccgta gatgacgggc ccacgacgac cggctcaccg
     4741 cgaccggctc acgagtagag cccgatcggt aggccgctgc cgcgcaggtg gaacgattcg
     4801 ttgacgctga cgatctggcg atgaccccgc gacgatgcga ccacccggtg gatcgaggtg
     4861 tagttcggat agaagaactg cgaccccggg gccaggccca acacgtgcgc caggtaggcg
     4921 ttgatcacac ccccgtggca gaccaccgcg atcttgatgc cgggatgggt ccggacgagc
     4981 gcgtcgagcg actcgatcac cctggtgatg aacgcgtcgc ggctctctgg gccgtcgagc
     5041 cagtcgccgt tgaccatctg ttgccaccgc gggtcgttgg tggcgcggag ctcttcgacg
     5101 gggatgtact cgctggcgtg ctggtcgaac tcggcgaccc cgtcgatgat ggtgacgtcg
     5161 aggccgacgc gcagggccag gggtgatgcg gtttgacgcg cccgcagcat cgggctggca
     5221 tagattgcct gcagctcctc ggtcgccaga tagtcaccga ggagttcggc ttgggcgcgg
     5281 cccgcctcgc tcacctcggg gtcggcagca ccggacagca actcccgccg caccggtagt
     5341 gcgtgacgaa ccatgatcaa ttccacaaca tcacatccct tcaccacgcc tccggcgatc
     5401 gtggccctca cgccgtcgat caccggcgta ctggcttatc agctatgacc ggccggacga
     5461 agatcgtggc cacgatcggc ccggccagcc aggcacccga gacgctacga gcgatgatcg
     5521 cggcgggtgt cgacgtggtg cggctcaacc tgagtcacgg ggagttgtcc gaacacatcg
     5581 agcgcctgca cgcggtgcgt gccgctgccg ccgacgtggg ccgtgtggtt gcggtgctgg
     5641 ccgacctgcc cggacccaag gtgcgcgcgg cgagctttcc cgacggggga gtgctgctgc
     5701 gggtgggcaa cctcatcacg ctcgcccctg gcgatcagcg cagcgatggc gaccggatcg
     5761 aggtcgacta cccgacgttg ctggccgaca tcgctcccgg cgaccgcatg gtcatcggtg
     5821 acggcggcat cgtgttgcgg gtggcgtcgg tcgacgtcgg tgaagcacac gccgtcgtcg
     5881 agtccggagg tctggtgcag ggccgtcccg gcgtgcacct acccagtgag cggctgcgcc
     5941 tgcgcacacc aaccgagcac gacctgaccc tggcgtgtgc gatggcggag gagggagtcg
     6001 acttcctcgg cgtgtcgttc gtgcgggctg ccgccgacct cgatgcggta cgggctgcga
     6061 tcgcgacgcg tttggtcgcc aagttcgaga ccaccccggc gatcaacgac atggaggcga
     6121 tcgtgggcaa cgccgatgcg gtcatggtcg ctcgcggcga tctgggcatc gagtgctcgc
     6181 tcgaagacgt gcctcacctg cagaagcgga tcatccgcca ctgtgtcgag atcggcgtgc
     6241 cggtcatcac cgcgacgcag atgttggaga gcatgatcgg cgcgcccgca cccacccggg
     6301 ccgaggtcag cgacgtggcc aacgcggtgt tcgacggtac cgacgcgctg atgctgtcgg
     6361 gcgagacggc cgtcggggcc gacccggtca acgtcgtcaa gacgatggcc cgcgtcgccg
     6421 aacgtgccga ggcgtcggcc aactacgccc ggtggagcga acggctcggc cgggtgcagc
     6481 agcgctgggc gcaccgggtc gatccgatca cggcggcgat cacccacgcc tgcgcccagg
     6541 cggccaacga tgcgggtgcc gaagcgatcg tgtgctgcac ccggtcgggc cgcacggcgg
     6601 cagcgatgtc ccgcttccgc ccaccggcgc tgttgatcgg ggccagcccc gacccggcga
     6661 cggtgcgggc gatggcgttg acgtggggtg tggtgccgat ccaggtcggg tcgtacggct
     6721 cgaccgacga actggtctgg ttcgcgatcg agnnnnnnnn nnaacagctc gtcgcccgcg
     6781 gctggatcgt cgccgtgctt gccggtgctc ccgaccgccc cagcggcgcg gccaccgacg
     6841 tcctccgcct cgtcagggtg tcgtgagccg atggggtgcg gatcacagcc gagcgggggc
     6901 acggcccccc agatcggcca gatctgggcg gacgaggcgg ggccggaaga cgcgccgctg
     6961 atcgcactgg tgcacgggtc gatggatcgc tcgacggggc tgttgcggtt gagccggcgg
     7021 ctcgacgatc ggtggcgggt gctgcgctac gaccggcgcg gctacgggcg ttcgctgcct
     7081 cacgacggtc cgtactcgat ggatcaccag gtcgccgact tggtcgcctt gttggccggt
     7141 cgtccagcgg tcatcctcgg ccatagctac ggtggcaacg tcgccctggc gacggcggcg
     7201 cgccatcccg agctcgtggc ggcgctcgcg ctgtacgaga cgccgttgtc gtgggagccg
     7261 tggtggccgg gagcgacggt gggttctacc gcactcgctg cgccgggtgg gccgcccgag
     7321 gcagcggaac ggttcatgcg ttcgttcatc ggtgacgacg cgtgggaagc gctgcccgac
     7381 aagacgcggg tgacgcggcg ccgcgagggg atcgcgatgg tgtgcgagtt gggtgacgtg
     7441 cgcgacaccc cgccatggca cgagtcggcg atcgcgtgtc cggtcgtgct cggctacggc
     7501 gcgaacggtg ccgcgcacca tgcggccggg atgcgccatc tgtgcgaact gctcaccggt
     7561 acgacgttgg tcgaggtgcc cgacgccgac catcgcgctc cccatgaccg cccgacagcg
     7621 atcgccgaga tgctggtcga tccggccatt cgtgccgcag gccagccctg gaccgacgcg
     7681 ctcagtctgg cgtgacgagc ggggctttga tcgtgatctc ctggccgaag aactggcaga
     7741 ccgcggtgcg gccgtcgggg atctcggcca cggagtcggc acagcgctgg agtgcggcgc
     7801 gctgggtgta gaccgcgaac gccaacgccg agcacagcgc gatggcgacc atcttgcgcg
     7861 agaagccgga gatcatcttg gccaggaacc cggccatcac caagagcgcg gcaatgatcg
     7921 cgagtccgat ccacttggcc gaggcaaggg tcaacgccac catcacgtcc atcgactgta
     7981 ctggcagccc catcgcccac ggaggaccga tgaccgcggg tagggtcggg ccggtgagcg
     8041 acgagcgagg ggtgcagttc agggggagcg ggttgccgtc cacggtgttg ccgccgatcg
     8101 atcaggcaac cgcagatgcg ttggcggcgg ctcgtgcttc tggcgagccg cgcgatgcga
     8161 tcgcggcggt ggtggcccgc gtcccacggt gtctcgacgc gtgggccgac ctgggcgatg
     8221 ccggacgtga tgtcgtcgag cgctactcgg cgtatcgcgt cggctaccac cggggtctcg
     8281 acgcgctccg tgccaatggc tggcgcggat cggggtatgt gcgctgggcc gaagcatcga
     8341 accgcgggtt cctgcgctcg ttgcgcgggt tgggtgcaat ggcggccgag atcggcgagc
     8401 acgacgaggc cgagcggatc gcggtcttcc tccgtcaact cgacccgtcg ggcatcccgg
     8461 cggcgtgacg cccatgccgg gcgctgctct cggtgccgtg ttcgtcggag gccagagccg
     8521 gcggatgggg accgacaagg cgctgatcga ggccgggggc gagcgcttgg tcgatcggac
     8581 cgcgcgcatc ctccgcgaag ctggctgcga gcaggttgcc gcgatcggac cacgacacct
     8641 cgctggcgat tatgtcggct cggtcggcct cgtcgccgac gaccatcccg gcgcaggacc
     8701 gctcggtgca ctgctgacgg cgctgcggct cgctgctcag gctggtgctg ccggtgtgat
     8761 ggtggcggcg tgcgaccttc ctgcgctcga cgtggcgacg gtggcggcgg tgctcgctgc
     8821 ggcgtcacca gggaacggcc acgtcggcat cgccgtcgcc gactcgggtc gccgtgagcc
     8881 gttgctgtcg tggtggcata cgcggacact cgccacggtg gaagcggcat tcgatgccgg
     8941 tgagcggtcg gtgctgcggg tgatcgaaca cgttgcgatg gggccggcga tgggaccggc
     9001 ggtgggcgcg gtggtcgatg ttccggtcgg cgcggacgcc gtcttcaaca tgaacacgcc
     9061 tgaccagcgg cccgagtgac tatcttcgct gccatggctg tgcaagaagt ttctctcgag
     9121 gccttcgccg ccgcgctcga agccgggttg gtcggccgcg tggtcgacgt ccgtgaaccc
     9181 ggcgagtacg ccgcggggca cgtgcctggt gcggtgctgg tgccgttggc caacgtgccc
     9241 gactcgatcg acgcctttgc cggcgacggg ccgacctacg tggtgtgcca ggtcggcggg
     9301 cctgccagta cctggccgac gaggatgtcg ccgcgatcaa cgtcgccgga ggcacagcgg
     9361 catggatcgc cagcggtcgc gccgtggcta ccggcgacga acccggaaac gtctgacctg
     9421 tgccaggggc gggcggcgac tacgactgga tcgacgacca ggtcggtctc gatcagctga
     9481 tcgacacact gctcggtcag ccgcgctacg ccttcgacac cgagttccac cgcgagcgca
     9541 cctacttccc ccgcttggcg ctggcccagt tcgcctggga cggtggtatc gccctggtcg
     9601 atccgctcgc ggtcgacgtc accgcgctgc agcggctgct gcacagcgac gtcgtggcgg
     9661 tgctgcacgc ggcccagcag gacctcgacg tcttgcagca cgcgtgtggt gctgtgccgc
     9721 ggcggttgtt cgacacccag ctcgcgtccg gcttcaccgg ctatggcacg ccgtcgctgt
     9781 ccagtctggt gcacggcgag ttgcacatcg cggtgcccaa gggcgaccgc ctcaccgact
     9841 ggctgcgccg tccgctgacg gccgatcagc gttcctacgc ggccagcgat gttgcctacc
     9901 tgctcgagct caccgatcgg ctcaacgaac ggctggagcg cgacggccgc ttcgcatggg
     9961 ccgaagcagc ctgcgaggaa ctgcgcacca ggccggtggg cggcgcggac ccggactcgg
    10021 cctggctgcg gatcaaggac gtccgcaccc tgaagccgcg tgcgcgtggc gtggcccggt
    10081 cggttgcggc gtggcgcgag cgacgggcga tggcggtcga caccccggtg cgccacatcc
    10141 tgcccgatct ggcgatcctg ggcatcgccc aacgccagcc acgcacggtc gaggatctcg
    10201 ctcacgcccg cggggtcgac gaacgccact ggcgcggtgc gctcggcaag gagatcatcg
    10261 ccgcagtagc cgaaggcgcg gggcacgaac tgcagatgcc cattggcgac agcgacgaac
    10321 tcgaccggca cctccgtccc gctgtcacct tggtcttggc ctgggtcagc gagctcgccc
    10381 gccaggagca catcgacacc gccctgttgg ccaccaagaa cgacatcgtg gcgttgctcc
    10441 gccgtgaccc ggacagccgc ctcgcctcgg gctggcgggc cgagatgctc ggcgacaacg
    10501 tcacccgctt ggtcgagggc cgggccgggt tgaccttcga cgggcggggt gggctgcgcc
    10561 tgatcgacgt gcccgccagc tgatcaccgg ggccggttgc tcagtctttg agctggcggc
    10621 gcagccgctt ccagagttgg tcgaacacgc tggacacgtc ggccgcacgg gcgccgtagc
    10681 tgtggatcga ccgccgctcg gcggtggcct ggttgacgat gacccgctgg gggatcgtcg
    10741 gctgccagat cgccttgcgt ccgacgatgc ggaccagttc gtcgtagcgt cgatcggcct
    10801 cgctggacac gccgggcact ttgttgacga tcaccccg
//
LOCUS       DAORNF010000015        10598 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_4660_length_10598_cov_1.857156, whole genome shotgun sequence.
ACCESSION   DAORNF010000015 DAORNF010000000
VERSION     DAORNF010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 10598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10598
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4660_length_10598_cov_1.857156"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="PLV68_01250"
     CDS             complement(<1..211)
                     /locus_tag="PLV68_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99889.1"
                     /translation="MRTAVATSHLSVTPGTPATLDVDVTNTADIIDGITATIQGLDPS
                     WVSLVVPVVSLFPQSSATLSLRIDLP"
     gene            complement(211..993)
                     /locus_tag="PLV68_01255"
     CDS             complement(211..993)
                     /locus_tag="PLV68_01255"
                     /inference="COORDINATES: protein motif:HMM:NF025431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pvc16 family protein"
                     /protein_id="HRD99890.1"
                     /translation="MLAAIDEAFEALLRATAPLSAVDIDVTFEQPDEEWVAKLTRPTV
                     NAYLWDVRRSSDRAVTGTEVAMRDNVPVRRLALPRVDLHYFVSVWTSEHDDERALLGS
                     LLLALLRNREIGADYLPGPLHGLPLPVVHVARTGDADTFRLDRRLKLGLQVTITAVAD
                     TGAGTPLAAPVTEIGVTLSDMSNGATATLPKRVAGECHDPAAIGARVTSPRGSTTVNA
                     SGRFLISAAPGDEITLDIDPAHTVVAPASGGVVFPLRDAAES"
     gene            1226..>1577
                     /locus_tag="PLV68_01260"
     CDS             1226..>1577
                     /locus_tag="PLV68_01260"
                     /inference="COORDINATES: protein motif:HMM:NF025215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation family protein"
                     /protein_id="HRD99891.1"
                     /translation="MLTGVLALGLLVGACSGDDDDPNAPQAEDVLKVDEGRCLLVDEA
                     LPPEVEKLPTIDCDKPHTHEIFATADDTEHDTYPGISVLSTFAEANCYGAFEDYVGIS
                     PYFGESSLSITWLVP"
     assembly_gap    1578..1620
                     /estimated_length=43
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2127..2190
                     /estimated_length=64
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2191..3460
                     /locus_tag="PLV68_01265"
     CDS             <2191..3460
                     /locus_tag="PLV68_01265"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRD99892.1"
                     /translation="HIERAGVLRALGAAVARRSEDLAQIWPRQSGVLYSMAQSAGPGA
                     QATLNGYADLASTFEFESPANPSNGGGFGLLVREPVGVVGAIIPWNGPMMLIAHKVGP
                     ALLAGCTVVLKLSPEAPGEGYVVAEAAEEIGLPPGVLNVVTADRDISELLVTDPRVDK
                     ISFTGSTAAGRRIASLCGERIARCTLELGGKSAAIVLDDADLATAAAKLAAAECRVSG
                     QVCASLTRIIVSAQRHDEMVEALAHEFRKVNVGNPFDPDVGIGPLATSRQRDRVEGYI
                     AAGLGAGATLAAGGGRPGHLDRGWFIEPTVFGHVESSMRIAQEEIFGPVLTVIPADDE
                     ADAVRIANDTIFGLNAAVFTNDVDHARDIAGRLRSGTVGHNGHRTDFDIGFGGFKQSG
                     LGREGGQQGLLAYLESKTILLDGPPSRWAD"
     gene            complement(3648..5084)
                     /locus_tag="PLV68_01270"
     CDS             complement(3648..5084)
                     /locus_tag="PLV68_01270"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRD99893.1"
                     /translation="MIQHRKSLAAFATVGCLLLAAGCRSDGKGEASTTTAAQTTTTAA
                     ASTTAAPSSTTASSTPDSGAESTTTTADSTPVTDPAPEAWTVDVSNCADPDAATAPIE
                     GEIKIGSAQPLSGTPAVAFAPVKAGIELYLAYANANNLIPDHTVSIDIRDDQYNPAET
                     PGVVSALIDDGVNLFAGIIGSQNNLNVRNTLNDECYPQLLALTGSPDWGDIENYPWTT
                     GALVPYDIEAKIYVDTMVDLGIKTTALFSVKNEFGNVFADAFKEAAAEAGIEVLDQQT
                     IEPAETTPPTNQVAAIAGKKVDAIMAIPLGAQCPTFLKEVENAEAVNAGWTPKIFLTN
                     TCASRLLISLLAGGAGDGVYTSTAGYDPEDPKYADIEGVVKFLEEYDNAGLEGDQGTT
                     AVGWSLAQSVVRILAKASESGTLTRQSIIEAARSMEFTNDLAFPGAVYKLDGDADPWT
                     AQSLQVVTWNEAAKTYDNVGDVVTKYEN"
     gene            complement(5101..>5677)
                     /locus_tag="PLV68_01275"
     CDS             complement(5101..>5677)
                     /locus_tag="PLV68_01275"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRD99894.1"
                     /translation="LLLIAYVVVRNLRKSRIGRSLVAVRDNETAAAVMGVNLRLTKTI
                     TFGVSAGLAAMAGWLYAAKAEAMDENSFTILLAIVYLLALFLGGAATLTGPIVGAFAY
                     YYLNKYVKDHAGDLGFLPGQLKNAAVAPLLLSAIVIVLVFIAPFGLVGFFKAQGRKLF
                     VVAPKPLRLTATTVLDEHTASEASADEDDHR"
     assembly_gap    5678..5687
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5688..6254)
                     /locus_tag="PLV68_01280"
     CDS             complement(<5688..6254)
                     /locus_tag="PLV68_01280"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRD99895.1"
                     /translation="MPVIIKENSPQHWAARVGTCAVIFGVIYYIYLTAQPSTLGSMTD
                     ATIIAIAAVALNLLLGYNGQISIGHSAFAGLAAFATGYTVNTHGWSPYVALLVSIVLC
                     FVFGFLVGLPALRLKGPYLALVTLAVAFVWPTIAAQFLTKKVKSTSLAGLELKPPKWT
                     GMGTERTNNSGVTRTVYTRLEVASFHYWL"
     gene            complement(6258..7178)
                     /locus_tag="PLV68_01285"
     CDS             complement(6258..7178)
                     /locus_tag="PLV68_01285"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRD99896.1"
                     /translation="MSDLIQYVINGLATGAVYSLVALGLVVIYRGTGHLNFAQGEMAL
                     FATFVAWWLYDQGLTMWFAAIVAALLSFVIGAVIEVGLMRPAGHKSPLAVVVVAIALF
                     QGLNSLTTLTFKGSADGLPFESLFPRKPTDFWRISDGIIWRYQHIGMLVTMLVLTGLL
                     FLLFQKTKIGLAMRMVANNPESARLVGVNTNRMLMFSWGLSSALGAIAGVLIGGFNTN
                     ITPATMFEIFLLASAAATLGGLDSPLGALVGGLSLGVIKSLVSSYPNDWPGGGYIGGP
                     TAIAVAFVIILIVLVIKPSGLFGSSRVERV"
     assembly_gap    7339..7441
                     /estimated_length=103
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7442..>8278)
                     /locus_tag="PLV68_01290"
     CDS             complement(<7442..>8278)
                     /locus_tag="PLV68_01290"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRD99897.1"
                     /translation="ERQLRDEAGDLLHSLQLSHLAFRPAMGLPFGTLKRLEIARALAA
                     RPTFLLMDEPASGLTHSEVAELSDTIVRVRDEFNLSVLLVEHHMAMVMSISDKVVAME
                     FGRKIAEGLPDEVRNNERVIAAYLSALLEVDQLVAGYGPIQVLHGLGLHVNEGEVAVM
                     LGANGAGKTTTMRAISGMIARKGRVRFNGKDIVKASADEIVRLGVAQVPQGRGTFTDL
                     SVEDNLRAGAFILKGNASAADLREWYDIFPRLEERKTQKAGSLSGGEQQMLAIGRALM
                     SRP"
     assembly_gap    8279..8288
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8289..8801)
                     /locus_tag="PLV68_01295"
     CDS             complement(<8289..8801)
                     /locus_tag="PLV68_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRD99898.1"
                     /translation="MATLDDNQDGTPDGSDQLGVTEVLDDTACSDGAVGPGHGAHHSH
                     HPNHDVYPLLDVQNVSVRFGGIVALDGLSFTIDDGQICGLIGPNGAGKTTMFNVISRI
                     YDATDGRVLFRNRDLLSVPAHGIAKRGIARTFQNLALFPTMTLLENVMVGAHSQGTAG
                     FWRSTLRIGTR"
     gene            complement(8961..9812)
                     /locus_tag="PLV68_01300"
     CDS             complement(8961..9812)
                     /locus_tag="PLV68_01300"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRD99899.1"
                     /translation="MTEPSPEPPAPSDPAATEQAAIGRARSRRERVLDNSIVRSVLSV
                     WFWLTFGIGVVVWLPLMAIVRLVTAPFDKGRYWTGYLFRKLPVVHQHLNPLWHFTVSG
                     DVPTDPRRPYVVVSNHESFVDILLISHLPFEMKWLSKVEMFKIPVVGWLMTLANDVRL
                     TRGEVSSAADAMDQCEQRLANKVSVMIFPEGTRSRSGDLGKFKNGAFRLAIDAGVPIL
                     PIAVHGAHRALRPKDWRIGYARAEVRVLAPIDTTGMTHSDIYALRDKARAAIVEGISL
                     IKADLGE"
     gene            9925..10563
                     /locus_tag="PLV68_01305"
     CDS             9925..10563
                     /locus_tag="PLV68_01305"
                     /inference="COORDINATES: protein motif:HMM:NF036808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99900.1"
                     /translation="MGTLSPALQGVPRPAAKRLAIRVSGDALRQIRGGHPWVFDRAIT
                     AMDGRSVGTDGGVAGDLGVVFDHDRRFAAIGLYDPHSPIRLRILHHGRPTTIDAAFFV
                     RRLAAAIERRATLADPTADQDTPLAGGSIERGDGETNAYRLVHGENDGFPGLVLDRYA
                     STLVLKLYTTAWLPHLHDLAPAIDAALAPRRLLVRFSRDAAGIRNIAQQPQL"
BASE COUNT         1730 a         3300 c         3551 g         1787 t
ORIGIN      
        1 tgggcaggtc gatgcgcagc gacagcgtgg cgctcgactg tgggaacagg ctgaccaccg
       61 ggacgaccag cgagacccaa ctcgggtcga gcccctggat cgtggccgtg atgccgtcga
      121 tgatgtcggc ggtgttggtg acgtcgacgt caagcgtggc cggcgtaccc ggcgtcaccg
      181 acaagtgcga cgtggcgacc gcggtgcgca tcagctctcc gcagcgtcgc gcagcggaaa
      241 gaccacgccc cctgacgcgg gggcgaccac ggtgtgggca gggtcgatgt cgagggtgat
      301 ctcgtcgccc ggcgcggcgc tgatgaggaa ccggccactg gcgttgacgg tggtcgaccc
      361 gcgaggtgat gtgacccgcg cgccgatcgc ggccgggtcg tgacactcac cggcgacccg
      421 cttcggcagt gtcgcggtag cgccgttgga catgtcggag agggtgacgc cgatttcggt
      481 gaccggtgcg gccagcgggg tacccgcgcc ggtgtcggcg acagcggtga tggtgacctg
      541 caagccgagc ttgaggcggc gatcgaggcg gaacgtgtcg gcgtcacccg tgcgcgccac
      601 atgcacgacc ggtaggggca ggccgtggag gggacccggc aggtagtcgg cgccgatctc
      661 gcggttgcgc agcagagcga gcagcagcga gccgagcaac gcccgttcgt cgtcgtgctc
      721 ggaggtccac accgagacga agtagtgcag atcgacgcgg ggtagtgcca gacgccgcac
      781 cgggacgttg tcgcgcatgg ccacttcggt tccggtgacg gcccggtcgc tgctgcggcg
      841 cacgtcccac aggtaggcgt tgaccgtggg gcgggtgagc ttggccaccc actcctcgtc
      901 gggctgttcg aacgtgacgt cgatgtcgac ggcactgagc ggtgccgtgg cgcgcagaag
      961 cgcctcgaac gcttcgtcga tggcggccag cattgctcag ccccttcgcg acacaggacc
     1021 tgcccagccc tcagccaccc ggcgagtcta accctccggt cgttcccggt cgctatgccg
     1081 tcccccgtca tctcgtgacg atgcggtgac ggtggtgtcg gcgcggtagc ggtgcgatcc
     1141 ggggcagtcg agccgggcgg gtacactcgc tgccattcgg gcaatgattc gggcttctcg
     1201 cgcgcggcat ggccggccgc gcgccgtgct gacgggtgtt ctcgcgctcg gtctgctggt
     1261 cggtgcgtgc agcggagatg acgacgaccc caacgcgccc caggccgagg acgtgctcaa
     1321 ggtcgacgaa ggccgctgct tgttggtcga cgaggccttg ccgcccgagg tcgagaagct
     1381 gcccacgatc gattgcgaca agccgcacac ccacgagatc ttcgccacgg ccgacgacac
     1441 cgaacacgac acctatcccg gcatctcggt gttgtcgacc ttcgccgagg cgaactgcta
     1501 cggcgcgttc gaggactacg tcggtatcag cccgtacttc ggcgagtcgt cgctgtcgat
     1561 cacctggctg gtccccannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 cccgccgcga cggcggccag ctcgaccagt cggtcaaggg cctcggcatc taacgacacc
     1681 tcagcaacac gcgcttgtgt ctgttgaccc agggagtggg ccatccctat aatcaatcat
     1741 tgatcggtta tgccgtgatc aatcgcgtcg ttgacgaacc ggggggatgc atgaccgttc
     1801 tggaatatcg atgtcgcgaa tcgacgtcgg tgggctccga acacggtcgc actctgggac
     1861 aacctcggta cgacgcacct cgtgccccgt gatgtgcgcg gtgcctcgtc cgccgatctg
     1921 agaccgatgc aagaaccgat ccgaaacccg atccgaaacc cgatccgaag aggagtgtcc
     1981 gtcgtgagaa ctgcaaacct gggcgcgatc gtgcgctcgc catctcggtt gttcatcgac
     2041 ggccagtggg tagcgcccgc gagcgacgcg atgatcgacg tgatcgacgc cgcagcgccc
     2101 tgagcccccg tttcgcgtcg atcgccnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ccacatcgaa cgcgccggcg tgctgcgcgc
     2221 cctcggggcg gcggttgccc ggcgcagcga agatctggct cagatctggc ctcgtcagtc
     2281 aggcgtgttg tattcgatgg cgcagtccgc cgggcccggt gcgcaggcga cgctgaacgg
     2341 ctacgccgac cttgccagca cgttcgagtt cgagagtccc gcaaacccaa gcaacggtgg
     2401 tggcttcggg ttgctggtgc gtgaaccggt cggcgtggtg ggcgcgatca tcccgtggaa
     2461 cggcccgatg atgttgatcg cccacaaggt cggtcctgcg ctgctcgctg gttgcaccgt
     2521 cgtgctcaaa ctgtcgcccg aggcccccgg cgagggctat gtggtcgccg aggctgcgga
     2581 ggagatcggc ttgccgcctg gcgtgctcaa cgtggtcact gcggaccgtg acatctccga
     2641 gttgctggtc accgatccgc gcgtcgacaa gatctctttc accggctcga cggcagccgg
     2701 tcggcggatc gcgtcgttgt gcggtgagcg gatcgcacgg tgcaccttgg agcttggcgg
     2761 caagtccgcg gcgatcgtgc tcgacgatgc cgatctcgcg acggccgcag ccaagctcgc
     2821 tgcggcggag tgccgggtgt cgggtcaagt gtgcgcatcc ctgacgcgga tcatcgtttc
     2881 agcacaacgt cacgacgaga tggtcgaggc gctcgcgcac gaatttcgaa aggtcaacgt
     2941 cggcaatccg ttcgatcccg acgtcggtat cggaccgctc gcgacgagtc gtcaacgcga
     3001 ccgcgtcgag ggctacatcg cggcagggct cggggccgga gcgacgctcg ctgccggtgg
     3061 tggcaggccc ggtcatctcg atcgcggctg gttcatcgag ccgacggtgt tcggtcacgt
     3121 tgagagttcg atgcgcatcg cgcaggagga gatcttcggg ccggtgctca ccgtgatccc
     3181 ggctgacgac gaggcagacg cggtgcgcat cgccaacgac acgatcttcg gtttgaatgc
     3241 tgctgtcttc acgaacgatg tcgatcacgc tcgcgacatc gccggccgcc ttcgttccgg
     3301 caccgttgga cacaacgggc accgtacgga cttcgacatc ggcttcggtg gcttcaagca
     3361 atccgggctt ggtcgcgagg gtggccaaca aggtctgctg gcctatctcg agtcgaagac
     3421 gatcctgctc gacggcccac catcgcgctg ggccgattga cacagttcgc acgaggacca
     3481 actgcggata cgtgtgagac gacgacaacc ggcgcgatgc cgaccaacat gccaggctca
     3541 acccgcgcgg cgtgctgctt cgatcactga acggggagcc gaaagcggcg actgccggcc
     3601 ccgaagggcc ggcagcgcac cgaatgggat gtgacccgac gaaggcgtca gttctcgtac
     3661 ttggtgacga cgtcgccgac gttgtcgtag gtcttggccg cttcgttcca ggtgacgacc
     3721 tggagcgact gggcggtcca cggatctgcg tcgccgtcca acttgtagac ggcgccgggg
     3781 aaggccaggt cgttggtgaa ctccatgctg cgcgccgctt cgatgatcga ctggcgggtc
     3841 agcgtgccgg actcggatgc cttggcaagg atgcgcacga ccgactgggc cagcgaccaa
     3901 cccaccgcgg tggtgccctg gtcgccttcg aggccagcgt tgtcgtactc ctcgaggaac
     3961 ttcacgacgc cttcgatgtc ggcgtacttc ggatcctctg ggtcgtaccc ggccgtcgag
     4021 gtgtacacgc cgtcaccggc accgccagcg agcaggctga tcagcaagcg cgaggcacag
     4081 gtgttggtca ggaagatctt cggcgtccaa ccggcgttga ccgcttccgc gttctcgact
     4141 tccttgagga aggtcgggca ctgcgcaccg agcgggatgg ccatgatcgc atcgaccttc
     4201 ttgccggcga tcgcggcgac ctgattggtc ggcggcgtgg tctccgcggg ctcgatcgtc
     4261 tgctggtcga ggacctcgat gcccgcctcg gcggcagcct ccttgaaggc gtcggcgaag
     4321 acgttgccga actcgttctt cacggagaac agtgcggtgg tcttgatgcc gagatcgacc
     4381 atggtgtcga cgtagatctt ggcctcgatg tcgtagggca ccagcgcacc cgtggtccac
     4441 gggtagttct cgatgtcacc ccagtcgggc gaaccggtca gcgccagcag ttgcgggtag
     4501 cactcgtcgt tgagcgtgtt gcgcacgttg aggttgttct gcgagccgat gatgccggcg
     4561 aacaggttga ccccgtcatc gatgagtgcc gagacgacgc cgggtgtctc tgctgggttg
     4621 tactggtcgt cgcgaatatc gatgctgacc gtgtggtcag ggatcaggtt gttcgcgttg
     4681 gcataggcca ggtacagctc gatgccagcc ttgaccggtg cgaaagccac ggcaggcgta
     4741 cccgacagcg gctgcgcgga gccgatcttg atctcgccct cgatcggcgc ggtggccgcg
     4801 tcggggtcgg cgcagttgct gacgtcgacg gtccaggcct cgggggccgg gtcggtcacc
     4861 ggggttgaat ccgcggtggt cgttgtcgac tcagcaccag agtccggcgt ggagctggcg
     4921 gtggtgctcg atggcgccgc agtggtgctg gccgctgcgg tcgtggtggt ctgggccgcg
     4981 gtggtcgtac ttgcctcgcc cttgccgtcg ctgcggcagc cagccgcaag caacagacac
     5041 ccaacggtgg caaacgccgc aagggatttg cggtgttgaa tcatcaaggt tcctcccctg
     5101 ttatcgatga tcgtcctcgt cggcggaagc ctcggaggca gtgtgctcgt cgagcacggt
     5161 cgtcgccgtg agccgcaatg gctttggtgc gaccacgaac aacttacgtc cctgagcctt
     5221 gaagaatccc accagaccga acggagcgat gaacaccagc acgatcacga tcgccgacag
     5281 cagcagtggg gcgaccgcag cgttcttcag ctgaccgggt aggaaaccca agtcgccggc
     5341 gtggtccttg acgtacttgt tgaggtagta gtacgcgaac gcgccgacga tcggcccggt
     5401 cagggttgct gccccgccca ggaacagggc cagcagatac acgatcgcga gcaggatcgt
     5461 aaagctgttc tcgtccatgg cctcggcctt ggctgcatac agccatccgg ccatcgcggc
     5521 caggcctgcg gagacgccga acgtgatcgt cttggtcagc cgcaggttga cgcccatcac
     5581 cgcagccgcg gtctcgttgt cgcgcaccgc gacgagcgaa cggccgatgc ggctcttgcg
     5641 caggttgcgc accacgacgt aggcgatcag caacagtnnn nnnnnnngag ccagtagtgg
     5701 aagctggcca cttcgaggcg ggtgtagacc gtgcgggtca caccgctgtt gttggtgcgc
     5761 tcggtgccca tgcccgtcca cttcggaggc ttgagctcga gcccggccag gctggtgctc
     5821 ttgaccttct tggtcaggaa ctgggccgcg atggtcggcc acacgaaggc cactgccaac
     5881 gtcacgaggg ccaggtacgg gcctttgagg cgcagggcgg gcaggccgac cagaaacccg
     5941 aacacgaagc acaacacgat cgacaccagc agcgccacgt acgggctcca gccgtgcgtg
     6001 ttgaccgtgt agccggtggc gaacgcggcc agaccggcaa acgcggagtg gccgatcgag
     6061 atctggccgt tgtacccgag cagcaggttg agcgcgaccg cagcgatcgc gatgatcgtc
     6121 gcgtcggtca tgctgcccag cgtggacggc tgtgcggtga ggtagatgta gtagatgacc
     6181 ccgaagatca ccgcgcaggt gccgacgcgg gcggcccagt gttgagggct gttctctttg
     6241 atgatgaccg gcatcgtcta caccctctcg actcgtgagc tgccgaacag gccggatggc
     6301 ttgatcacca gcacgatcag gatgatcacg aacgccaccg cgatcgccgt ggggccgccg
     6361 atgtagccac cgccgggcca gtcgttggga tagctggaca ccaggctctt gatgacaccc
     6421 aaggacagcc caccgaccag cgcgccgagc gggctgtcga gtccgcccaa ggtggctgct
     6481 gccgaggcga gcaggaagat ctcgaacatc gttgccgggg tgatgttggt gttgaagccc
     6541 ccgatcagca caccggcgat cgcgccgagt gccgacgaca gaccccaact gaacatcagc
     6601 atccggttgg tgttgactcc gaccaggcgg gccgactcag ggttgttggc caccatccgc
     6661 atcgccaggc cgatcttggt cttctggaag agcaggaaca gcagcccggt gagcaccaac
     6721 atcgtgacga gcatgccgat gtgctggtag cgccagatga tgccgtcgct gatccgccag
     6781 aagtcggtgg gcttgcgcgg gaacaggctc tcgaaaggca agccatcggc cgagcccttg
     6841 aacgtcagcg tggtcaacga gttgagcccc tggaacaggg cgatcgccac gaccaccacc
     6901 gcgagcggtg acttgtggcc ggccgggcgc atcagtccca cctcgatcac ggcgccgatc
     6961 acgaagctga gcagcgccgc cacgatggct gcaaaccaca tggtgaggcc ttggtcgtag
     7021 agccaccagg cgacgaaggt ggcgaacagc gccatctcgc cttgggcgaa gttgaggtgg
     7081 ccggtaccgc gatagatgac gacgaggccc aacgcgacca gggagtagac cgccccggtg
     7141 gcgaggccgt tgatgacgta ctggatgaga tcggacatgg ctggctcctc agtaccccag
     7201 gtaggccttg cgaacgacgt cgttggcggc gatctcttcg gccgttccgg atgcaacgat
     7261 caggcccgtc tccagcacga acacgctctg ggcgatcttc aacgccaggt tggcgttctg
     7321 ctcgaccacc aggatcgann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7441 ngggacggct catcagcgcc cggccgatgg ccagcatttg ctgttcgcca ccggagaggc
     7501 tgcccgcttt ctgggtcttg cgctcttcca ggcgggggaa gatgtcgtac cactcccgca
     7561 ggtcggccgc cgatgcgttg cccttcaaga tgaaggcacc agcgcggagg ttgtcttcga
     7621 ccgacaggtc ggtgaacgtg ccccggccct gtggtacttg ggccacgccg agacggacga
     7681 tctcgtcggc ggaggccttg acgatgtcct tgccgttgaa gcgaaccctg cccttgcggg
     7741 cgatcatgcc ggagatggcc cgcatggtgg tggtcttgcc ggcgccgttg gcgcccagca
     7801 tgaccgccac ttcgccttcg ttgacgtgca ggcccaggcc gtggagcact tggatcgggc
     7861 cgtatccggc gaccaattgg tcgacctcga gcagcgcgct cagataggcg gcgatcactc
     7921 gctcgttgtt gcggacttcg tcgggcaggc cctcggcgat cttgcggccg aactccatgg
     7981 cgacgacctt gtcggagatg ctcatcacca ttgccatgtg gtgctcgacc aacagcaccg
     8041 acaggttgaa ctcgtcgcgg acccgcacga tcgtgtcgct cagttcggcg acctcgctgt
     8101 gggtcaggcc ggacgccggt tcgtccatca gcaagaacgt cggccgggcc gccagcgcgc
     8161 gcgcgatctc cagccgcttg agcgtgccga agggcaggcc catcgccggg cggaacgcga
     8221 ggtggctcag ctgcaagctg tgcagcaggt cacccgcctc gtcgcggagt tggcgctcnn
     8281 nnnnnnnngc gggtgccgat gcgcagggtg ctgcgccaga aacctgccgt gccttgactg
     8341 tgcgcaccga ccatcacgtt ctcgagcaac gtcatggtgg ggaacagggc caggttctgg
     8401 aaggtccggg cgatgccccg cttggcgatg ccgtgggccg gcaccgagag caggtcgcga
     8461 ttgcggaaca ggacccggcc gtcggtggcg tcgtagatgc ggctgatgac gttgaacatc
     8521 gtcgtcttgc ccgcgccgtt ggggccgatg agaccgcaga tctgcccgtc gtcgatcgtg
     8581 aacgagaggc cgtcgagggc cacgatgcca ccgaagcgca ccgacacgtt ctgcacgtcg
     8641 agcaacgggt agacgtcgtg gttggggtga tgggagtggt gggcgccgtg tcccgggccg
     8701 actgcaccgt cgctgcacgc cgtgtcgtcc aagacctcgg tgacgcccaa ctggtcgcta
     8761 ccgtcgggcg tgccgtcttg attgtcgtca agcgtcgcca tgcttgattg cccccctttc
     8821 ggtggcgaga ccatagcgca gcgagggatg cagacgaaga gtggagtgac tccttcgacc
     8881 gattttctca gcgccgctga ttgccgtgcc gagccgcccg ccgcgctggt cagtaggcgc
     8941 gcgacgcacc cgttgccggg tcactccccg aggtcggcct tgatcagcga gataccttcg
     9001 acgatcgcgg cgcgggcctt gtcacgcagg gcgtagatgt cggagtgggt catgccggtg
     9061 gtgtcgatcg gggcgagcac gcggacttcg gcacgggcgt agccgatgcg ccagtctttg
     9121 gggcgcaacg ctcggtgcgc gccgtgcacg gcgatcggca ggatcgggac acccgcgtcg
     9181 atcgccaagc ggaaggcacc gttcttgaac ttgcccagat cgccggagcg gctgcgcgtc
     9241 ccttcgggaa agatcatcac gctgaccttg ttggccaagc gctgctcgca ctgatccatc
     9301 gcatcggcgg cgctcgacac ctcgccgcgg gtgaggcgga cgtcgttggc gagcgtcatc
     9361 agccaaccga ccaccgggat cttgaacatc tccaccttgc tcagccactt catctcgaac
     9421 ggcagatggc tgatcagcag gatgtcgacg aacgactcgt ggttggacac caccacgtac
     9481 ggtcgccgcg ggtcggtcgg gacgtcgccg ctgaccgtga agtgccacag cgggttgagg
     9541 tgctgatgca ccaccggcag cttgcggaac aggtagccgg tccagtagcg acccttgtcg
     9601 aacggcgcgg tgaccagccg cacgatcgcc atcaacggca gccagacgac gacgccgatg
     9661 ccgaaggtca gccagaacca caccgacagc accgagcgga cgatggagtt gtcgagcaca
     9721 cgctcgcgtc ggctgcgcgc ccgcccaatc gccgcctgtt cggtcgccgc aggatcggac
     9781 ggggcagggg gctcgggcga tggctcggtc acagcggcat tctcgcacgc gaccccaccc
     9841 tgtgaccgcc ccggtgggca tcgacgtgtg ccagcccgga gcaggcgaag cgcagtggga
     9901 cggagggttc ggcatactgg ctcgatgggc actctctctc cggcgttgca gggtgtgccg
     9961 cgcccggctg ccaagcggct ggcgatacgg gtcagcggtg atgcgctgcg ccagatccgg
    10021 ggtgggcatc cgtgggtgtt cgaccgtgcg atcaccgcaa tggacggccg cagtgttggc
    10081 accgatggcg gtgtggcggg tgacctgggc gtcgtgttcg accacgaccg acggttcgcg
    10141 gcgatcgggc tgtacgaccc ccactcgccc atccgcctgc gcatcctgca tcacggacgg
    10201 ccgacgacga tcgacgccgc gttcttcgtg cggcgcctgg cggccgcgat cgaacggcga
    10261 gcaacgctgg ccgacccgac cgctgaccaa gacactccct tggccggcgg gtcgatcgag
    10321 cgcggtgacg gcgagacgaa cgcctaccgg ctcgtgcacg gagagaacga cgggtttcca
    10381 ggactggtgc tggaccgcta cgcgtcgacg ctggtgctga agctgtacac caccgcctgg
    10441 ttgccccatc tgcacgacct cgcccccgcg atcgacgccg cgctcgcgcc caggcggttg
    10501 ctcgtccgct tcagccgtga tgcggccggg ataagaaaca tcgctcaaca accacaatta
    10561 tgatttgcgt ctctcagttt gggagaccca aaaactca
//
LOCUS       DAORNF010000016        10497 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_4721_length_10497_cov_1.866692, whole genome shotgun sequence.
ACCESSION   DAORNF010000016 DAORNF010000000
VERSION     DAORNF010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 10497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10497
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4721_length_10497_cov_1.866692"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="PLV68_01310"
     CDS             complement(<1..239)
                     /locus_tag="PLV68_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99901.1"
                     /translation="MVELPWCGLGGDAFVLVRTADGGTFGFNGSGVAPPLPGANGLAA
                     PLTDTQLYNTIRDGANHGADIGGPGAKANLTDKQI"
     assembly_gap    242..382
                     /estimated_length=141
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(416..802)
                     /locus_tag="PLV68_01315"
     CDS             complement(416..802)
                     /locus_tag="PLV68_01315"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HRD99902.1"
                     /translation="MAEILPIIAPGLVAPTGPFPHATRIGHRVYTSGQAGRNRDTGQM
                     GDAAEQVDGALRNIAVVAEAAGTAIEHTLKVTLILRDDVTDMAAVNEAYARHFPDRQP
                     ARTSFFVSRLKNPDMLVEVEAVIGVV"
     gene            complement(842..1849)
                     /locus_tag="PLV68_01320"
     CDS             complement(842..1849)
                     /locus_tag="PLV68_01320"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRD99903.1"
                     /translation="MAERLLSIEQAVVRYDTPSGAITPVDGVDLVINEGETLGLVGES
                     GCGKSTLGRLIIQLARANSGAVHFAGRDLQAMSASQLRSLRKELQVIFQDPRGSLDPK
                     MSIRQIVAEPLKVHGMAKGDERNRKADEILEMVGLSSSIGHRRPGQLSGGQQQRVGIG
                     RALITEPRLVVCDEPVSALDVSVQAQVINLLQDLKERLGVAYLFISHNLAVVRHLSDR
                     VAVMYLGKIVEQGPSHDVFARPLHPYSRGLLASVLRPDTDAPERLQQATSFIIGDVPS
                     FHDVPSGCRFHPRCPYASDRCREEQPVLEFATGEHAREHSVACHHWEEIIPMPVAVRA
                     K"
     gene            complement(1842..>2129)
                     /locus_tag="PLV68_01325"
     CDS             complement(1842..>2129)
                     /locus_tag="PLV68_01325"
                     /inference="COORDINATES: protein motif:HMM:TIGR01727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99904.1"
                     /translation="PYSAALIRLVPKMDQRVHHRLRPIPGQPPISIGALEGCRFAPRC
                     EYRQDRCAVEPPIIELGPQHSAACWRAEELVDVMHAAHDHADHAEAVANHG"
     assembly_gap    2130..2178
                     /estimated_length=49
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2179..2855)
                     /locus_tag="PLV68_01330"
     CDS             complement(<2179..2855)
                     /locus_tag="PLV68_01330"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRD99905.1"
                     /translation="MPLLEVEDLRCSFLTPSGEVRAVDGISFAMEAGESLGVVGESGS
                     GKTMTALSMLRLFGPSSTVRLAGSVRFDGIDLLRVNDKTLRTIRGGKIGMVFQDPLTS
                     LDSVMPVGAQIAESLRLHKGLSRKAANHRAIELLERVGIPNAVHRAKDLPHRFSGGMR
                     QRIMIAIAISCEPQLLIADEATTALDATVQMQILALLQDLQRDLGMAMMVISHDLGVI
                     AALCETA"
     gene            complement(2855..3730)
                     /locus_tag="PLV68_01335"
     CDS             complement(2855..3730)
                     /locus_tag="PLV68_01335"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRD99906.1"
                     /translation="MSANVIVVDRPAELTHRSLFSRIRPYGWLIWAVILGAILLCVLF
                     PNVIAPFDHAKQAPRDKFLSPWSTGARGLHLFGTDELGRDVFSKVVAGARLTLFIGLM
                     STLIGATIGATLGMIAGLRGGWIDRIVMRLTEAQTAMPMFLIALLLLSTLGPSVWNLI
                     MILPTYVWPTFARLIRAETLRMRTSPFIEAAESLGCRTRTILWRHLLPNLWTRVAVLA
                     AVSVGQVILSEAGLSFIGAGVQRPDVTWGLLISQGRKYLTVAWWATVFPGLFAGLTVF
                     AINAIARRFSQGRER"
     gene            complement(3730..4068)
                     /locus_tag="PLV68_01340"
     CDS             complement(3730..4068)
                     /locus_tag="PLV68_01340"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRD99907.1"
                     /translation="MVDELNKSYVTVATSKGLGYRTVVTRHIMRNVGLVLVTMVGWEY
                     ARMWGGTVFLAEFTFAWPGIGKLVTDAASRHDFHVIQAGVVVAGVFVVLANLVVDLLA
                     TMVDRRVEAS"
     assembly_gap    4477..4594
                     /estimated_length=118
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4823..6460)
                     /locus_tag="PLV68_01345"
     CDS             complement(4823..6460)
                     /locus_tag="PLV68_01345"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRD99908.1"
                     /translation="MIRRIPTKRLAASVFAVGLLLAACGSDDKDSTTTTTQTTTAPST
                     GDSSSPSSSTASSTAPSTTADTPSSSSEPAADVKPLVIAVSTEPSTLDAQLVNDRSSR
                     VVTSSMYEALLFRSAEGEIVPLLATEYEATSDTTWSFTLRDDVTFHNGEPFNAEAAAF
                     SINRIISEEFGTQRTSYTDGIVEATATDEFTVEVETDGILATLPVQLTQIPMVPPGAA
                     DTLDTDPVGTGPYKFVEWAPGVEIVATRNDAYWGEAPAIKDYIVRVVADNQTALSALQ
                     SGEVDLVIDLLPEQMDQAPVALSVPASEFSYIAFNTYLPALSDPRVRVAFNLAIDKDL
                     LAETVYEGQARPNEAQHLAEGMLGFNPDLGPFPYDPEGAKALLDEAGFDYNTEITLNV
                     PIGRYLKGEETAEFVAAQLEEIGVKIKVNAIEFATFREEGRIPGDEPGAMDLKYAWNS
                     NEFGDGSRIIAHIICGGSSSKICIPEVDELMEAAGSTVDQDARDADYQEVWAILHENP
                     HSIYLLQQNLIYGTTERLTWEPRVDDEYYVATMALTS"
     gene            complement(6513..>6765)
                     /locus_tag="PLV68_01350"
     CDS             complement(6513..>6765)
                     /locus_tag="PLV68_01350"
                     /inference="COORDINATES: protein motif:HMM:NF015862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HRD99909.1"
                     /translation="AFGKARLAAAFRKPTATLLEQWKDRPLFAQSLTEVIPQGVTLNP
                     GGYPIFLDDECIGAFGVGGGSPQQDEHLARRISAWWDAR"
     assembly_gap    6766..6802
                     /estimated_length=37
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6948..8201)
                     /locus_tag="PLV68_01355"
     CDS             complement(6948..8201)
                     /locus_tag="PLV68_01355"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRD99910.1"
                     /translation="MRQGYKVIDTDTHVTPSIEVLMRYADDELKARADELVQYTRVTK
                     PVAGRGHPTEDYGVVRVNPYPYERMAGRKYGETEEPAGAGARGALEGRTENEATRSVH
                     TRIQHDNPEGRLLDMDVEGVDIDLIIPGTWAPGSSALDVDLAKSMHRAYHRYMTDYTN
                     ADPRRLKGIILADTRDPKGTAKEIEELAAHDWVAGVWPVLPEGLPIDDPDLSPIFAAM
                     NEANLPIVHHSFFYEAPYFPGYRDVWGNAAFARTAAHVWGAQRLLGYVLISGMLDRFP
                     NLRVGCVETGHGWLANFIIRLDKQIKFVKGGTVPPELQHTPLEFVEMGRVFCAIEAFE
                     GPVMTKGVIDVLGEDVLMFESDFPHPECMFPETTDHIIGWKSILGESATRKLMGENAM
                     KFLRLTTTPWDDEAKPSTTTMPLVD"
     gene            complement(8201..8965)
                     /locus_tag="PLV68_01360"
     CDS             complement(8201..8965)
                     /locus_tag="PLV68_01360"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HRD99911.1"
                     /translation="MRETLADRMSSQLRQQILSGRLAPGQAMPTERELGDAFGVGRTT
                     VREALRGLIHSGFLERRGHTLLVRDRNEIGADDVSLAEMAARISVEDLYDTRKALEAH
                     AVALAARNWADGDIDALREALEQMKGKLGADYHAADVAFHMLIARIGKNAVMQQVYEM
                     ATNLFFKLPSFWRVFSPQQGPAKPITGYDGHRSVVDAIEARDADLAVRLSDELLDRVK
                     HTLIERMNRDGSASADAVAETVDTPTETPTMKRGNA"
     gene            complement(9131..9940)
                     /locus_tag="PLV68_01365"
     CDS             complement(9131..9940)
                     /locus_tag="PLV68_01365"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRD99912.1"
                     /translation="MHLQRGGDEDDSAAVIAATYTVWQHLFAALAAIGGGLVNAVAGG
                     GTLISFPVLTAIGVPSVRANATNTVALLPGYLGGVYAQRSDLAGLSKQIKPQLVTALI
                     GGLIGSVLLIVTSDSAFRAIVPFLILSAVILMAVQDRIRTAIFKPGQQHSDHPVLQIV
                     SIFAAAVYGGYFGAGLGIMLLAVLGLFSDRPFSQINAVKLILAFSANLTAAIFLSFSG
                     KVVWSIALVMIPAALLGGNLGGRVARIVPAKKMRAFVIAFGFVVAIIYFVK"
BASE COUNT         1729 a         3241 c         3477 g         1705 t
ORIGIN      
        1 tagatctgct tgtcggtgag attggctttg gcgcccggtc cgccgatatc agcgccgtga
       61 ttcgcgccat cgcggatcgt gttgtagagc tgcgtatcgg tgagcggcgc cgcgagtccg
      121 ttcgcccccg gcaacggcgg cgcgacgccg ctgccgttga agccgaacgt gccaccgtcg
      181 gcggtgcgca ccaggacgaa cgcgtcgccc ccgaggccgc accacggcag ttcgaccaca
      241 cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nncctgttct cagacgcggt cgggaaccag agccgtcaga
      421 cgacaccgat gaccgcctcg acttcgacga gcatgtccgg gttcttcaac cggctgacga
      481 agaacgacgt ccgcgcgggc tggcgatcgg gaaagtggcg cgcgtaggcc tcgttgactg
      541 cggccatgtc ggtgacgtcg tcgcgcagga tcagggtgac cttcagcgtg tgttcgatcg
      601 ccgtgccagc ggcttcggcg acgacggcga tgttgcgcag cgccccgtcg acctgttcgg
      661 cggcgtcgcc catctggccg gtgtcgcggt ttcgtcccgc ctgaccggac gtgtagacgc
      721 ggtggccgat gcgggtggcg tgggggaagg gcccggtcgg ggcgaccagc cccggggcga
      781 taatgggaag gatctcagcc atcggtgttc ctccgggtgg cgatcgaggg atgggaagcg
      841 ttcacttggc acgcaccgcg acgggcatcg ggatgatctc ttcccagtgg tgacaggcaa
      901 cgctgtgttc acgagcgtgt tcgccggtgg cgaactcgag cacgggttgc tcctcgcggc
      961 agcgatcgga cgcgtagggg cagcgagggt ggaagcggca gccgctcggc acgtcgtgga
     1021 agctggggac gtcaccgatg atgaacgacg tcgcctgctg caaccgctcg ggagcgtcgg
     1081 tgtcgggacg cagcaccgac gcgagcagac cgcggctgta cgggtgcagg gggcgggcga
     1141 acacgtcgtg cgaggggccc tgctcgacga tcttgccgag atacatcacc gcgacccgat
     1201 cggagagatg gcgtacgacg gcgaggttgt gcgagatgaa cagataggcg acgccgaggc
     1261 gctccttcag gtcttgcaac aggttgatga cctgggcctg caccgacacg tccagcgccg
     1321 agaccggctc gtcgcaaacg acgagacggg gttcggtgat cagcgcgcgg ccaatgccga
     1381 cccgctgctg ctgaccgccg gacaactggc ctggccggcg atggccgatc gacgagctga
     1441 gcccaaccat ttccaggatc tcgtcggcct tgcggttgcg ctcgtccccc ttggccatgc
     1501 cgtgcacctt gagcggttcg gccacgatct ggcggatcga catcttgggg tcgagcgagc
     1561 cgcgcgggtc ttggaagatg acctgcagct ctttgcgcag cgatcgcagt tgggacgcgc
     1621 tcatggcctg gaggtcgcgc ccggcgaagt gcaccgcacc cgagttggcc ctggcgagct
     1681 ggatgatcaa ccgaccgagc gtcgacttgc cgcaaccgct ctcgccgacc aacccgaggg
     1741 tctccccctc gttgatcacg aggtcgactc cgtcgaccgg ggtgatcgcg cccgacggcg
     1801 tgtcgtagcg gacgacggcc tgctcgatcg acaacaaccg ttcagccatg gttggccacc
     1861 gcctccgcgt gatcggcatg gtcgtgagcg gcgtgcatga cgtcgaccaa ctcttccgcg
     1921 cgccagcagg cggcgctgtg ttgtggcccg agctcgatga tcggtggctc aacggcgcag
     1981 cggtcctggc ggtattcgca gcgcggcgcg aaccgacaac cttcgagtgc cccgatcgag
     2041 atcggcggct ggcccgggat ggggcgcaga cggtggtgca cacgttggtc catcttgggt
     2101 accaggcgga tcagcgccgc gctgtatggn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnntg agccgtctcg cacagcgctg cgatcacgcc caggtcgtgg
     2221 ctgatcacca tcatcgccat gccgaggtcg cgctggagat cctgcaacaa cgccaagatc
     2281 tgcatctgca ccgttgcgtc gagcgcagtc gtcgcctcgt cggcgatcaa caactggggt
     2341 tcgcacgaga tcgcgatcgc gatcatgatt cgctggcgca tgccaccgct gaagcggtgc
     2401 ggcagatcct tcgcgcgatg gacggcgttg gggatgccga cccgttcgag cagttcgatc
     2461 gcccgatggt tggcagcctt gcgactcaga cccttgtgga gtctgagcga ctcggcgatc
     2521 tgggcaccga ccggcatgac cgagtcgagc gaggtgagcg ggtcttggaa caccatgccg
     2581 atcttgccgc cgcgaatggt gcgcaacgtc ttgtcgttga cccgcaacag gtcgataccg
     2641 tcgaagcgca ccgacccggc caggcgcacc gtgctgctgg ggccgaacag gcgcagcatg
     2701 ctgagtgccg tcatggtctt gccgctgccg ctttcgccga cgacgccgag cgactcgccg
     2761 gcctccatcg cgaacgagat gccgtcgacg gctcgcacct caccgctcgg ggtcaggaac
     2821 gagcagcgca gatcttcgac ttcgagcaac ggcatcagcg ctctctcccc tggctgaaac
     2881 gacgggcaat cgcgttgatc gcgaacacgg tgagaccggc gaacagaccg gggaacaccg
     2941 ttgcccacca tgcgacggtc aggtacttgc ggccctgaga gatcaacagg ccccacgtga
     3001 cgtcaggccg ctgcacgccg gcgccgatga acgacagccc ggcctcggac aggatcacct
     3061 ggcccacgct gaccgcggcc aacaccgcaa cgcgggtcca caggttgggc agcaggtggc
     3121 gccacaagat ggttcgcgtg cggcatccca gcgattcggc cgcctcgatg aacggcgacg
     3181 tgcgcatgcg caaggtctcg gcgcggatca gacgggcaaa ggtcggccag acgtaggtgg
     3241 gcaggatcat gatcaggttc cacacgctcg gcccgagggt ggacagcagc aacagggcga
     3301 tcaggaacat cggcatcgcg gtctgggcct cggtgaggcg catcacgatg cggtcgatcc
     3361 agccgccacg caagcccgcg atcatgccca gcgtcgctcc gatcgtagct ccgatcagcg
     3421 tgctcatcag cccgatgaac agcgtgagac gagcgccggc gaccaccttg ctgaatacgt
     3481 cgcggcccag ttcgtcggtg ccgaacaggt gcaggccgcg ggcgccggtc gaccacggcg
     3541 acaggaactt gtcgcgcggc gcctgcttgg cgtgatcgaa cggcgcgatc acgttgggga
     3601 acaacacgca gagcaggatc gcgcccagga tcacggccca gatcagccag ccgtagggcc
     3661 ggatgcggct gaacagactg cgatgagtca gctcggccgg gcggtcgacg acgatgacgt
     3721 tggcagacat caggaggcct ccacccggcg atcgaccatg gtcgcgagca ggtcgacgac
     3781 gaggttggcg agcaccacga acacgccggc gacgacgaca ccggcttgga tgacgtggaa
     3841 gtcgtggcgg ctggcagcgt cggtgaccag cttgccgatg cccggccagg cgaaggtgaa
     3901 ctcggccaag aacaccgtgc caccccacat ccgggcgtac tcccagccga ccatggtgac
     3961 gagcaccagg ccgacgttgc gcatgatgtg gcgggtgacc acggtgcggt agccgagccc
     4021 cttgctggtc gcgacggtga cgtacgactt gttcaactcg tcgaccacgg acgagctgga
     4081 agaggcggcc gccgtggtgg atgccgagcg tgacccccgg gaggatgtag ctcgacaacc
     4141 ccccgtcggg taagacggag acccagcccc atcgcaccgc gaagatcaga atgaatatct
     4201 gtccgaacca gaagtagggg aacgacaccc ctgccatcgc gaacaggttg gcggcacggt
     4261 cgaacagtga tccgggcttg acgcccgcga tcgcaccggt gaccacaccg aacaacacgg
     4321 agaagaacag cgccgcaacc gtcaagcgca tggtggcgcc gaggcggtcg aggatcagcg
     4381 gcaacgctgg gcggttgcgg gccaccacca gcgagtcgcc gaggtcgccc gtgagcaacc
     4441 cggtgtagta ctccttggcc tggaccagca cgggccnnnn nnnnnnnnnn nnnnnnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnaagacg acgaacaaca cgatcacccc
     4621 atcgcgcaga cggcggagaa cgagcatccc ccgagaccga ccccgccgcc gcagccgctc
     4681 gcgaacgaca gcagcgggac cgggggtcgg gctctgtcca tcaggggtat tcgcgagatc
     4741 gggcgtggca gtggtcatgg gtctcggggg gctcgtcagc tgtgctgtgg ggtgggttgg
     4801 gtgaacgctg aagcgggcta gctcacgagg tgagggccat ggtcgcgacg tagtactcgt
     4861 cgtcgacgcg gggctcccag gtgaggcgct cggtggtgcc gtagatcagg ttctgctgca
     4921 gcaggtagat cgagtgcggg ttctcgtgca ggatcgccca cacctcttga tagtcggcat
     4981 cacgtgcgtc ctgatcgacg gtggaacccg cggcttccat cagctcgtcc acttcgggga
     5041 tgcagatctt ggagctcgaa ccgccacaga tgatgtgagc gatgatgcgg ctgccgtcac
     5101 cgaactcgtt cgagttccag gcgtacttca gatccatcgc accgggctca tcgccgggga
     5161 tgcggccctc ctcacggaag gtggcgaact cgatcgcgtt gaccttgatc ttgacgccga
     5221 tctcttcgag ctgagcagcg acgaactccg ccgtctcctc acccttcaag tagcggccga
     5281 tcggcacgtt gagcgtgatc tcggtgttgt agtcgaagcc tgcctcgtcg agcagcgcct
     5341 tggcaccttc gggatcgtac gggaacggac cgaggtcggg gttgaaaccg agcatgccct
     5401 cggcgaggtg ctgggcctcg ttcgggcggg cctggccttc gtacaccgtc tcagccaaga
     5461 ggtccttgtc gatcgccagg ttgaaggcga cgcggacacg aggatcgctc aacgcgggga
     5521 ggtacgtgtt gaaggcgatg tagctgaact cggaagccgg caccgacagc gcgaccggag
     5581 cctggtccat ctgctcggga agcaggtcga tcaccaggtc gacctcgccc gactgcagcg
     5641 ccgacaaggc cgtctggttg tcggccacga cgcggacgat gtagtccttg atcgccggag
     5701 cctcgcccca gtaggcgtcg ttgcgggtgg cgacgatctc gacgcccggt gcccactcga
     5761 cgaacttgta cggtcccgta ccgaccggat cggtgtcgag cgtgtcggcc gctcccgggg
     5821 gcaccatcgg aatctgggtg agctgcaccg gcagcgtggc caggatgccg tcggtctcga
     5881 cctcgacggt gaactcgtcc gtcgctgtcg cttcgacgat gccgtcggtg tagctggtgc
     5941 gctgggtacc gaactcctcg ctgatgatgc ggttgatcga gaaggcggcg gcctcggcgt
     6001 tgaacggctc accgttgtgg aacgtgacgt cgtcgcggag cgtgaagctc cacgtcgtgt
     6061 cggacgtggc ctcgtactcg gtggcgagca gcggcacgat ctcgccctcg gcggagcgga
     6121 acaacaaagc ctcgtacatg ctgctggtga ccacgcggct gctgcggtcg ttgaccaact
     6181 gcgcgtcgag cgtcgacggt tcggtcgaca cggcgatcac gagcggcttg acgtcggctg
     6241 ccggttccga cgacgagctc ggcgtgtcgg cggtggtgct gggtgcggtg ctggacgcgg
     6301 tggacgacga cgggctggac gaatcaccgg tggacggtgc ggtcgtcgtc tgcgtggtcg
     6361 tggtcgtact gtccttgtcg tcgctgccgc aagctgccaa cagcagcccg acagcgaaca
     6421 ccgaggcggc aagccgcttg gtgggaatcc tgcggatcat tcgggtgtct ccttgtgggg
     6481 ttgggtctgg ttgcgggatc cacgtccggc ggttaccggg cgtcccacca cgcactgatt
     6541 cgacgagcga ggtgctcgtc ctgttggggg gaacctccac cgacgccgaa tgcaccgatg
     6601 cactcgtcgt cgaggaagat cgggtagccg cctgggttca acgtgacgcc ttgagggatc
     6661 acctcggtca ggctctgcgc gaacagcgga cggtccttcc actgctccaa caaggtggca
     6721 gtgggcttgc ggaaagcggc agccagacgg gccttgccga aggcgnnnnn nnnnnnnnnn
     6781 nnnnnnnnnn nnnnnnnnnn nnacggggtg gccagcggca tcgacgatcg cgatcgtcgc
     6841 gacgagaccc tcttccgcgg cgatctcgcg cgccgcggtg atcatcgcca gtgcgtcgtc
     6901 ggtgtccatg cccgctcccc tgatcccgtt cgccgcgtcc gtcggtttca gtcgaccaac
     6961 ggcatcgtgg tggtcgacgg cttggcctcg tcgtcccacg gggtggtggt gaggcgcagg
     7021 aacttcatcg cgttctcgcc catcagcttg cgcgtcgcgc tctcgccgag gatcgacttc
     7081 cagccgatga tgtggtccgt cgtctcgggg aacatgcact cgggatgggg gaagtcggac
     7141 tcgaacatca gcacgtcttc gccgagcacg tcgatgactc ccttggtcat gaccgggcct
     7201 tcgaaggcct cgatcgcgca gaacacccgg cccatttcga cgaactcgag cggggtgtgc
     7261 tgcagctctg gtggcacggt gccgcccttg acgaacttga tctgcttgtc gaggcggatg
     7321 atgaagttgg ccagccagcc gtgaccggtc tcgacgcagc cgacgcgcag gttggggaag
     7381 cggtcgagca tgccgctgat cagcacgtag ccgagcagac gctgggcacc ccacacgtgg
     7441 gcggcggtac gggcgaaagc cgcattgccc cacacgtcgc gatagccggg gaaatacggc
     7501 gcttcgtaga agaagctgtg gtgcacgatc ggcaggttgg cctcgttcat cgcggcgaag
     7561 atcggcgaga ggtcggggtc gtcgattggc agaccctcgg gcagcacggg ccacacgccg
     7621 gccacccagt cgtgcgcggc cagctcttcg atctccttcg cggtgccctt cggatcgcgc
     7681 gtgtcggcca ggatgatgcc cttcaaccgt cgcgggtcgg cgttggtgta gtcggtcatg
     7741 taacggtggt aggcgcggtg catcgacttg gcaaggtcga cgtcgagcgc cgagctgccc
     7801 ggcgcccatg taccggggat gatcaggtcg atgtcgacac cctcgacgtc catgtcgagc
     7861 agacggccct cggggttgtc gtgctggatg cgggtgtgga cggagcgggt tgcctcgttc
     7921 tcggtgcgac cctcgagggc accccgagcg ccggcaccag ccggctcttc ggtctcgccg
     7981 tacttgcgcc cggccatccg ctcgtacggg tacgggttga cgcgcaccac gccgtagtcc
     8041 tcggtcgggt gaccacgccc ggccaccggc ttggtgactc gggtgtactg cacgagctcg
     8101 tcggcgcgcg ccttgagctc gtcgtcggcg tagcgcatga gcacctcgat gctcggcgtg
     8161 acgtgggtgt cggtgtcgat gactttgtat ccctgacgca tcaggcgttc cctctcttca
     8221 tggtgggggt ctcggttggg gtgtcgacgg tttcggccac ggcgtcggcg gatgccgagc
     8281 cgtcgcggtt catccgctcg atcagggtgt gcttgacccg atcgagcaac tcgtcggaga
     8341 ggcgcacggc caagtcggca tcgcgcgcct cgatcgcgtc gacgacgctg cggtggccgt
     8401 cgtagccggt gatcggcttg gccgggcctt gctgggggct gaacacccgc cagaagctcg
     8461 gcagcttgaa gaacaggttg gtcgccatct cgtagacctg ctgcatgacg gcgttcttgc
     8521 cgatgcgggc gatcagcatg tggaaggcga cgtcggcggc gtggtagtcg gcgcccaact
     8581 tgcccttcat ctgctcgagt gcttcgcgca gggcgtcgat gtcgccgtcg gcccagttgc
     8641 gagcggcgag ggcgacggcg tgagcctcga gcgccttgcg ggtgtcgtac agatcttcga
     8701 ccgagatccg ggccgccatc tcggccaggc tcacgtcgtc ggcaccgatc tcgttgcggt
     8761 cgcggaccag cagcgtgtgg ccgcgccgtt cgaggaaccc gctgtggatc aggccgcgaa
     8821 gcgcctcgcg caccgtggtg cgcccgacgc cgaacgcgtc gccgagctcg cgctcggtgg
     8881 gcatcgcctg gcccggagcg agccgaccgc tcaggatctg ctgccgcagc tgcgacgaca
     8941 tccggtcggc aagcgtttca cgcaccaaca gcaaatcttc gtctggcacc cggtccccct
     9001 cgtgatctct cgttccggcg tctgttcgtg agactgtctg gtaagcagat tgtcagacaa
     9061 tctgctcgtc tgcacgaatg taggccgcga tgcagcctgg acacaagtgg aacgacgaaa
     9121 tttcttcggg ttacttcacg aagtagatga tcgccacgac gaagccgaac gcgatcacga
     9181 acgcccgcat cttcttcgcg ggcacgatgc gcgccacgcg gccgccgagg ttgccgccca
     9241 gcagcgcggc cgggatcatc acgagcgcga tcgaccagac gaccttgccc gagaacgaca
     9301 ggaagatggc cgcggtcagg ttggcactga aggcgaggat cagcttgacg gcgttgatct
     9361 ggctgaaggg acgatccgag aacaggccga gcacggccag cagcatgatg cccaggcctg
     9421 cgccgaagta gccgccgtac accgccgcgg cgaagatcga cacgatctgc agcacagggt
     9481 ggtcgctgtg ctgctggcca ggcttgaaga tcgcagtccg gatgcggtcc tgcaccgcca
     9541 tcaggatcac ggccgacaag atcaggaacg gcacgatcgc gcggaacgcg gaatcgctcg
     9601 tcacgatcag caacaccgat ccgatcaagc cgccgatcag ggccgtgacc aactgcggct
     9661 tgatctgctt gctcagtcca gccaggtcgg accgctgcgc gtagaccccg ccgaggtagc
     9721 ccggcaacaa tgcgaccgtg ttggtcgcgt tggcccgcac cgacggcacg ccgatcgccg
     9781 tcaacaccgg aaagctgatc agcgtgccac cccctgcaac ggcgttgacg agcccgccgc
     9841 cgatcgcggc cagcgccgcg aagaggtgtt gccagacggt gtaggtagca gcgatcaccg
     9901 cagcagaatc gtcctcgtca cccccgcgct gcaaatgcaa tcctctcacc ctgccccgcc
     9961 ggacaacccc tcccctcgcg tccgccagcg gcacgttgga tgggcacata cctcgcgaag
    10021 cacacgttgc agcgagcaga ctcgtcgagc cagaggtcgg ttcgacggtc gcgagcgcga
    10081 tcgttgcatc tgcaatgact gcccggaaag catggagtag cagggtctga ggccaatcgt
    10141 tggagcagtc gtccaccgag tcgacgttga accgcatcat cgccacggag tcgcgaacaa
    10201 tgtcatcgaa cgtgtcgttg tggatccggc acatccgcat tctgtgctca ccgacgaccc
    10261 tcttcgatga cgttgatctg tcgacaagcc gctctgcggc cagcctgggc gtgccagact
    10321 cggcggatga tcgagcgcga gatcgaggtc acgacgtccg aagggacgat gaagaccttc
    10381 gtgtaccacc cgagccacgg cgggccgcac ccggtggtgc tgtacctgat ggacgccccc
    10441 agcatccgcc ctgccttgat ggacatggcc tcccggctgg ccaccgccgg gtactac
//
LOCUS       DAORNF010000017        10353 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_4796_length_10353_cov_1.532239, whole genome shotgun sequence.
ACCESSION   DAORNF010000017 DAORNF010000000
VERSION     DAORNF010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 10353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10353
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4796_length_10353_cov_1.532239"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1621
                     /locus_tag="PLV68_01370"
     CDS             <1..1621
                     /locus_tag="PLV68_01370"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HRD99913.1"
                     /translation="TGGRVHATDATNASRTMLFNIHEGVWDDELLRIFGVPRGLALAG
                     VALIWFSPSVAWWSLNPIGTGLAYGVAAALAFVVAAEHLARLSGGPPAQRQRTIWRVG
                     ALIAVGGVLCARVPFAYAPWSIWLAASVLAPAAVVIVQQLGWRALRWPAVAAVAWVAM
                     LGLGVLVENRTGLSTLNATAYPARRRETAESLDTARLFGAPFLGVLGRGHGVVNTNES
                     ELSSAWLILVVPCLLSALVVPGNVLRRARAVWWALAAVTAALLAWVTIDLPAPIGTRL
                     PVIDLSPAYRIAQIVGIPATVLFVLTVVRMRGEAAAVRRRVAVGSVIVSAVVLAMAGS
                     TMREFYVPGLSPTRVLVVTIVVALAIGVAIEFAPRAWSSAPLVVLAIATTAHVNPLQV
                     GTEPLTGGEAHAVITAMQATADGRWASDTQAVDAVLMANAVPSLSGQQWVGPRPEAWR
                     VLDPSGAAEERWNRGSAYIVLRWAVDDLAPRIANPAPDVIDVTLSPCDPALDVFGVRW
                     VVASGPLDAPCLTPAATFTLGAHEQFVYARE"
     gene            1684..2835
                     /locus_tag="PLV68_01375"
     CDS             1684..2835
                     /locus_tag="PLV68_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxide hydrolase"
                     /protein_id="HRD99914.1"
                     /translation="MSEISSESGLEPFTIAIADDVLDDLRRRLATTRWPAEMADDEWV
                     YGTNQAVLRDICASWQQFDWRAVEARLNSYRQIVTAIDGQRVHALLAPSPEPDALPLL
                     LTHGWPGSIVELLDVIEPLRDPVAHGGRAGDAFHLVIPSLPGYGFSGPTTDNGWDVRR
                     VAEAWKVLMARLGYDRYLAQGGDWGSMVSSQLAVIDAEHMAGLHVNMVLGIPGDQALS
                     ETEQAQLAHTGAFMAEGTAYQQIQGKNPHTLGYGLTDSPAGLAGWIVEKFHHWTHHPD
                     GDWETAVSRDQLLANLTLYWVTNTIASSVRLYFESQRSGRFPPADRVEVPTGCAVFPG
                     EIISLPESWARNLYNVTRYTMFDRGGHFAAMEEPDLFVDDVRAFFATVR"
     gene            2889..>3293
                     /locus_tag="PLV68_01380"
     CDS             2889..>3293
                     /locus_tag="PLV68_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99915.1"
                     /translation="MIRRVLLAMCVGATVLAIGQPSQAGGPPSTATSASPTALPPSPA
                     AVPARPEVVDPSRRVFMLTDSVGLGVRGVLQRALPEYQVTIDGFPALMVDQLDDRMAK
                     PRVARNSTDIGDIAIIAAGYNYPYWDPARFDRD"
     assembly_gap    3294..3464
                     /estimated_length=171
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3465..>4352
                     /locus_tag="PLV68_01385"
     CDS             <3465..>4352
                     /locus_tag="PLV68_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99916.1"
                     /translation="EMQLADWASITDQPGVTYDAIHLNSTGQQLYSSMLATLVRTSDT
                     WWSARSEHAVTVAGLNGVPADAKAVAVNLTVTTPRSPGFLTAWACGEPMPPTSNLNFM
                     SDQTVAVSAVVNVGTGGQICIFNSTDTQVIVDVQGYFGPDSDYVTVAPSRVIDTRDDG
                     GVHAAGEPLAVRVTGVADVPDDAAAVAVTVTVADNTAPGWVLSYPCDEPQTTPIALVN
                     YIQYTATPSFALVKPGADGTICLRSLTPANLIVDVFGYLPAGSSVSVMAPTRLLDTRG
                     GAPIPALTDVVVPVVGVEG"
     assembly_gap    4353..4380
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4381..4614
                     /locus_tag="PLV68_01390"
     CDS             <4381..4614
                     /locus_tag="PLV68_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99917.1"
                     /translation="TGIGWVVAYPCGTAAATSTLNVVPQRAVTNSTLVAPGAGGAVCL
                     KANVTTHFLFDVSAWVFDGYTGLTPWRALDSRG"
     gene            complement(4646..5506)
                     /gene="rsmI"
                     /locus_tag="PLV68_01395"
     CDS             complement(4646..5506)
                     /gene="rsmI"
                     /locus_tag="PLV68_01395"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HRD99918.1"
                     /translation="MTPQTGTTQAGGTLVLVATPIGNLGDTSSRAVDALRTAALICCE
                     DTRRTAKLLNHFGIAGVRMAVANEHTEAARVDQVLGVLGAGQTVAVVSDAGMPGISDP
                     GGLLVRAAIEAGHTVSAVPGPSAEVMALVISGLATARYVFEGFLPRSGGERTARIAEV
                     AAERRTVVLYEAPHRLARTVADLAAACGGERAVAIARELTKVHEEVWRGSLADAVVMV
                     ASREPQGEYVVVLAGAPAPVAADDAAIRAALIERLAAGGSTKSAVLAVADALGVAKNR
                     VYDVALTVDR"
     gene            complement(5554..5898)
                     /locus_tag="PLV68_01400"
     CDS             complement(5554..5898)
                     /locus_tag="PLV68_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HRD99919.1"
                     /translation="MPWLMSNARVLASADIADSPRQRSKGLLGRDSIDGALIIPRCRW
                     VHTVGMRFDIDVAYVDATNVVVKTETVRRHRVAMPVPKAQMVIEAAAGAFERWGLRVG
                     DPVEVRVAEPDT"
     gene            complement(5931..6437)
                     /locus_tag="PLV68_01405"
     CDS             complement(5931..6437)
                     /locus_tag="PLV68_01405"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRD99920.1"
                     /translation="MHIRPAIVDDAAAIHDMVLAIASETRLDQVVTSTADDIARHGFG
                     VWPEFHTLIAEHDGEPVGMCLYFRHFSTVRGKVGGYIQDLYLQPQHRGSGLAARLLEA
                     ASAHIHEVGGTFLRLSVDADNIRGQRFYAKHGMNWSEDERIFQVDGADFAAMATRGQH
                     IAAEPGAR"
     gene            6558..>7039
                     /locus_tag="PLV68_01410"
     CDS             6558..>7039
                     /locus_tag="PLV68_01410"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HRD99921.1"
                     /translation="MPGSSSHERTIPHQPALDGVRAVAVAVVLLFHLGLSWMPGGYLG
                     VSVFFTLSGFLITALVVREVEHTGRVSIGAFYTRRLKRLLPASLTCLAAVSVLGAAGV
                     WTGVANLRRDLLGALLQVANWVQLLAGESYTELQSKRAGIISPLDHYWSLAIEEQFYW
                     L"
     assembly_gap    7040..7059
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7091..>7577
                     /locus_tag="PLV68_01415"
     CDS             7091..>7577
                     /locus_tag="PLV68_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99922.1"
                     /translation="MRRRGRAVPLGSMVVMTVACAIAAPLIARWWGADAAYWASPARA
                     GEILVGATLAVALHAGRIHPTRWMAPVGLVGVIALGVALPATGGPAYQGWMTPLALVS
                     GLLIVGLQRPGPVRQLLGSRPFAVTGRVSYGLYLYHFPVFVALDEHRLGFGGVGLFVI
                     RI"
     assembly_gap    7578..7603
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7706..>8105
                     /locus_tag="PLV68_01420"
     CDS             7706..>8105
                     /locus_tag="PLV68_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99923.1"
                     /translation="MPSAASANYFQVDSELAHQAQIETPADGSLAPLVVATTTTTAPT
                     VSAPASSAVSSTTTVTDSPTAPTTPTAPTATAMPTTTVVPATDSVPPTVVGLYDVVVT
                     PVLNRPVRIVVLGDSTAVALGAGLVRWAAER"
     assembly_gap    8106..8117
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8118..>8391
                     /locus_tag="PLV68_01425"
     CDS             <8118..>8391
                     /locus_tag="PLV68_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99924.1"
                     /translation="ALDGCGFIHGGTGADNPFTVPCQEHLDVELPASIPAAQPDVVVL
                     MSTLGDFGPRTWDDGGIDLQPTDPEYLVRLQAGYTAISELIESTTNA"
     assembly_gap    8392..8506
                     /estimated_length=115
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8819..9271
                     /locus_tag="PLV68_01430"
     CDS             8819..9271
                     /locus_tag="PLV68_01430"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HRD99925.1"
                     /translation="MSRAEQFELLPDEVAQRWRKFGRSERIFYTQLLGIEVEEVRVDY
                     CRMRLPFRPELQQPAGLVHGGALASLLDAVVVPAIGAVYEADVRFATVDMHVQFMSAV
                     RDHDVVGEGWVTRRGRTTAFCEAEAVDAVTGATVARSMLTYNIANPAG"
     gene            9463..>10353
                     /locus_tag="PLV68_01435"
     CDS             9463..>10353
                     /locus_tag="PLV68_01435"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HRD99926.1"
                     /translation="MSIPQAAAAATEIGFPVVVKLCGDAIAHKTERGLVKLRLGDAEA
                     VRTASEELLAKATPDDGDVSVLVAPMIAGNRELIAGMLRDAQFGATIMLGVGGILAEA
                     IADVVFRPAPLTRLEAGEMIDGLATQALLQPFRGEAAVNRDALIDVLVGLGDIAEARP
                     DVASIDVNPLIVRADGMPIAVDALVEIATPATATADTHVVPDLALFDALFNPRGVVVT
                     GASTHPGKFGFVSLHNILASGYQGKVFGTNLKGEEVLGITTVRDLDELPEGEVDLAFV
                     CTPAATNPDLLRACAAKGIRA"
BASE COUNT         1470 a         3520 c         3385 g         1606 t
ORIGIN      
        1 gaccgggggc agggtgcatg cgaccgatgc caccaatgcc tcgcggacga tgctgttcaa
       61 catccatgag ggcgtctggg acgacgagct gctgcgcatc ttcggcgtgc cgcgcggcct
      121 tgcgctggcg ggcgtcgcgc tgatctggtt cagcccctcg gtggcgtggt ggtcgctcaa
      181 cccgatcggt accggactcg cctacggcgt tgccgccgca ctcgcgttcg tcgttgccgc
      241 ggaacacctg gcgcggctca gcggggggcc acccgcccaa cgacagcgca cgatctggcg
      301 cgtgggcgcg ctgatcgcgg tcgggggcgt gctgtgtgcc cgggttccgt tcgcctacgc
      361 gccctggtcg atctggctcg cagcatccgt tctcgctccg gccgcggtgg tgatcgtgca
      421 acaactcggc tggcgggctc tgcgctggcc agcggtggcg gcggtggcgt gggtggcaat
      481 gctgggtctg ggcgtgctgg tcgagaaccg aaccgggttg agcaccctga acgcgacggc
      541 ctacccagcc cggcggcgcg agacggccga gtcgctcgac actgcccgac tgttcggggc
      601 gccgttcctg ggcgtactcg gccgcggtca cggcgtggtc aacaccaacg agtcggaact
      661 gtcgagcgcg tggctgatcc tggtggtgcc gtgtctactc agcgcgttgg tggtgccggg
      721 caacgtcctg cgtcgagcgc gcgccgtgtg gtgggccctc gccgctgtca ccgccgcgct
      781 cctcgcgtgg gtcaccatcg atctgcccgc accgatcggt acccgcctgc ctgtcatcga
      841 tctcagcccc gcctatcgca tcgcccagat cgtcgggatc ccggccaccg tgctgttcgt
      901 cctgaccgtg gtccgtatgc gcggcgaagc agcggccgtt cgtcgccgtg tcgcggtcgg
      961 ttccgtgatc gtcagcgcgg tcgtactggc gatggccggc tcgaccatgc gcgagttcta
     1021 cgtgccgggt ttgtcaccga cccgcgttct ggtcgtcacc atcgttgtcg cactggcgat
     1081 cggcgttgcg atcgagttcg ccccgcgcgc atggtcctcc gcaccactgg tcgtgctggc
     1141 gattgcgacg accgcccacg tcaacccgtt acaggtcggc accgaaccgt tgaccggcgg
     1201 cgaggcgcac gcggtgatca ccgcgatgca ggcgaccgct gacggacgct gggccagcga
     1261 cacgcaggcc gtcgacgccg tactgatggc caacgcggta ccgtcgctgt ccggacagca
     1321 gtgggtcggc ccgcggcctg aagcgtggcg agtactcgac ccctccggtg cggccgaaga
     1381 acgatggaac cgcggctcgg cctacatcgt gctgaggtgg gcggtcgacg acctggcgcc
     1441 caggatcgcc aacccggccc cggacgtgat cgacgtgacg ctgtcgccgt gcgaccccgc
     1501 gctcgacgtg ttcggggtgc gctgggtggt ggcctccggc ccgctcgatg ctccctgcct
     1561 gacgccggcg gcaacgttca cgctcggcgc gcacgaacag ttcgtctacg cgcgggagta
     1621 acaaaagcgg atggccacgc gcccggctcg ttggtgtgcg ggctgggtcg tgacaatggc
     1681 gcgatgagtg agatctcctc cgagtccggg ctcgaaccgt tcaccatcgc gatcgccgac
     1741 gacgtgctcg atgatctgcg gcggcggctg gccaccacgc gctggccggc cgagatggcc
     1801 gacgacgagt gggtctacgg caccaaccag gcggtgctgc gcgacatctg cgcgtcgtgg
     1861 cagcagttcg actggcgcgc ggtcgaggcg cggctgaaca gctatcgcca gatcgtcacc
     1921 gccatcgacg gccagcgggt gcacgccctg ctcgcaccct ctccggagcc ggacgcgctg
     1981 ccgctgctgt tgacccacgg atggccgggg agcatcgtcg agttgctcga cgtgatcgag
     2041 ccgctgcgcg accccgtggc ccatggcggc cgggctggcg atgcgttcca cctcgtcatc
     2101 ccctcgctgc ccggctacgg gttcagcggt cccaccaccg acaacggctg ggacgtgcgc
     2161 cgcgtcgccg aggcgtggaa ggtgttgatg gcccggctcg gctacgaccg ctatctcgcc
     2221 cagggcggcg actggggctc gatggtgtcg tcgcagttgg cggtgatcga cgccgagcac
     2281 atggccggcc tgcacgtcaa catggtgctc ggcatccccg gcgaccaggc gctgtccgag
     2341 accgagcagg cccaactcgc acacaccggc gcgttcatgg ccgagggcac cgcctaccaa
     2401 cagatccagg gcaagaaccc gcacacgctg ggctacgggc tgaccgattc ccccgcgggc
     2461 ttggccggat ggatcgtcga gaagttccac cactggaccc atcaccccga cggcgactgg
     2521 gagacggccg tctcccgtga ccaactgttg gccaacctga cgctgtactg ggtcaccaac
     2581 acgatcgcgt ccagcgtgcg gttgtacttc gagtcgcagc gctccggtcg atttcctccg
     2641 gccgatcgcg tcgaggtgcc caccgggtgc gcggtgttcc ccggcgagat catctcgcta
     2701 cccgagtcgt gggcccgcaa cctgtacaac gtcacccgct acacgatgtt cgaccgcggc
     2761 ggtcacttcg cggcgatgga ggagcccgac ctgttcgtcg acgacgtccg cgccttcttc
     2821 gccacggtgc gctgacccgt tccagtgttg ccggccacgc aggtgccagg atggcgaatg
     2881 tgcaacgagt gatacgacgg gtcctgctgg cgatgtgtgt cggagcaacg gtgctggcga
     2941 tcggccaacc gagccaagcc ggcggaccgc cgagcacggc cacgtctgca tcacccaccg
     3001 ccctgccgcc atcgcccgca gccgtgccgg cccgacccga ggtggtcgac cccagccgac
     3061 gggtgttcat gctgaccgac tcggtcggac tcggcgtgcg tggcgtgctg cagcgagcgc
     3121 tgcccgagta ccaggtgacg atcgacggtt tcccggcgtt gatggtcgac caactcgacg
     3181 atcggatggc caagccgcgc gtggccagga actcgactga catcggcgac atcgccatca
     3241 tcgccgctgg ctacaactac ccctactggg acccggcccg gttcgatcgc gacnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncgagat gcaactcgcg
     3481 gactgggcgt cgatcaccga tcagcccggc gtcacctacg acgcgatcca cctcaacagc
     3541 accggccagc agctctacag ctcgatgttg gcgacgctcg tgcgcacgag cgacacatgg
     3601 tggtcggcgc gctccgagca tgccgtcacg gtcgccgggt tgaacggggt gcctgccgat
     3661 gccaaggcag tcgcggtcaa tctcaccgtt acgaccccgc gctcgccggg attcctgacg
     3721 gcttgggcgt gcggcgagcc gatgccgccg acctcgaacc tcaacttcat gtccgaccag
     3781 accgtggccg tgtcggccgt ggtcaacgtc ggcaccggcg gccaaatttg catcttcaac
     3841 agcaccgaca cgcaggtgat cgtcgacgtg cagggctact tcggccccga cagcgactac
     3901 gtcaccgtgg caccgagccg ggtgatcgac acccgtgacg acggcggcgt gcacgcagca
     3961 ggcgaaccac tggcggtacg cgtgaccggc gtggccgacg tgcccgacga tgccgccgcc
     4021 gtggcggtca ccgtcacggt cgccgacaac acagcgcccg gctgggtgct gtcgtacccg
     4081 tgcgacgagc cgcagaccac cccgatcgcg ctcgtcaact acatccagta caccgccaca
     4141 ccaagcttcg ccctggtcaa accgggcgcc gacggcacca tctgtctgcg ctcgctgacg
     4201 cccgccaacc tgatcgtcga cgtgttcggg tacctgcccg ccgggtcgag cgtgtcggtg
     4261 atggccccga cgcggttgct cgacacacgc ggtggcgcgc cgattcccgc gctgaccgat
     4321 gtggtcgtgc cggtggtcgg cgtcgagggg ttnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4381 accggcatcg gctgggtggt cgcctacccg tgtggcaccg cggcagcgac gtccacgctc
     4441 aacgtcgtac cgcagcgagc ggtgaccaac tcgaccctcg tcgcccccgg tgccggtggc
     4501 gccgtctgct tgaaggccaa cgtgacgacg cacttcctgt tcgacgtctc cgcctgggtg
     4561 ttcgacggct acaccggcct caccccgtgg cgggccctcg acagccgcgg ctgagggttc
     4621 ccgatcgccc ctcaacggtc aacggtcagc ggtcgacggt cagcgcgacg tcgtagaccc
     4681 ggttcttggc cacaccgagc gcgtcggcca cggccaagac ggctgacttc gtggaaccac
     4741 ccgcagccag gcgctcgatc agcgcggcgc ggatcgccgc gtcgtcggcc gctaccggcg
     4801 caggcgcacc agcgagcacg accacgtact caccctgcgg ctcgcgtgag gccaccatca
     4861 ccaccgcgtc ggccaacgag ccgcgccaca cctcttcgtg caccttggtg agttcgcggg
     4921 cgatggccac cgctcgctca ccgccgcacg ccgcggccag gtccgcgact gtgcgggcga
     4981 ggcggtgcgg cgcctcgtac aacacgacgg tgcgccgctc tgcggcgacc tctgcgatcc
     5041 gggcagtgcg ctcgccaccg ctgcgcggca gaaagccctc gaacacgtag cgcgccgtcg
     5101 ccagaccact gatcaccaac gccatcacct cggccgacgg gccgggcacg gccgacacgg
     5161 tatgaccggc ctcgatcgcg gcccgcacca acagcccacc cgggtcgctg atgccgggca
     5221 tgccggcgtc gctgaccaca gccaccgtct ggccggcacc caacacgccg agcacctggt
     5281 cgacccgtgc ggcctcggtg tgctcgttgg ccacggccat gcgcaccccg gcgatgccga
     5341 agtggttgag caacttggct gtgcggcgtg tgtcctcgca acagatcaac gccgcggtgc
     5401 gcaacgcgtc gacagcgcgc gacgacgtgt cgccgagatt gccgatcggc gtcgcgacca
     5461 gcaccaacgt gccccccgcc tgcgtcgttc ccgtctgcgg cgtcaccctc gatgccgtgc
     5521 ccctcgatac cgtccccctc gatacaggca tggtcacgtg tccggctcgg ccacgcggac
     5581 ttcgaccggg tcgccgacgc gcagccccca tcgctcgaag gcacccgccg cggcctcgat
     5641 caccatctgt gccttgggca ccggcatcgc cacccggtga cggcgtacgg tctcggtctt
     5701 gaccaccaca ttggtggcat cgacgtaggc gacgtcgatg tcgaagcgca tgccgacggt
     5761 gtgcacccag cggcaacggg ggatgatcag cgccccgtcg atgctgtcac gccccaacag
     5821 acccttgctg cgttggcgcg gtgagtcggc gatgtcggcc gaggcgagca cccgcgcatt
     5881 gctcatcaac cagggcatgg gcacagcctg tcatgcacgc cggacatgga tcagcgcgcg
     5941 ccgggctcgg ctgcgatgtg ctggccgcgg gtcgccatcg cggcgaagtc ggccccgtcg
     6001 acctggaaga tccgctcgtc ttcggaccag ttcatgccgt gcttggcgta gaaccgctgg
     6061 ccgcggatgt tgtcggcgtc gaccgacagg cgtaggaacg tgcccccgac ctcgtggatg
     6121 tgggcggagg cagcttcgag caaccgtgcc gccaggccgc taccccggtg ttggggctgc
     6181 aggtagaggt cctggatgta gccgccgacc ttgccccgca cggtggagaa gtggcggaag
     6241 tagaggcaca tgcccaccgg ctcgccatcg tgttcggcga tcagggtgtg gaactcgggc
     6301 cacaccccga acccgtgacg ggcgatgtcg tcggcagtgg aggtgaccac ctggtcgagg
     6361 cgggtctcgg aggcgatcgc gaggaccatg tcgtggatgg cagccgcgtc gtcgacgatg
     6421 gcgggtcgaa tgtgcatctg ggcgatcttc tgtcggcgcc gcggacgcgg cgaacggcaa
     6481 ttgtgggccg cccgggcgcg ttcgccaaca taccggaacc gatcgaggcg ttgcagtgcc
     6541 gtctacagtg acccggcgtg ccgggatcga gcagccacga acgaacgatc ccgcaccagc
     6601 cggctctcga cggtgtccgt gcagtcgccg tggccgtggt gctgctattc cacctgggtc
     6661 tgtcgtggat gccgggtggc tacctcggcg tgtcggtgtt cttcacgctg tcagggtttc
     6721 tgatcacggc gctggtggtg cgcgaggtcg aacacaccgg ccgggtgagc atcggcgcgt
     6781 tctacacccg tcgcctcaaa cgactgctgc cagcgagcct cacgtgcctg gcggcggtca
     6841 gcgtgctcgg cgccgccggc gtctggacgg gtgtcgccaa cctgcgccgc gacctgctcg
     6901 gtgcgctgct gcaggtggcc aactgggtcc agttgctggc gggggagagc tacaccgagc
     6961 tccagagcaa gcgggcaggg atcatctcgc cgctcgacca ctactggtcg ttggcgatcg
     7021 aggagcagtt ctactggctn nnnnnnnnnn nnnnnnnnnc gtagggcgag aggcggatcg
     7081 cgtgctcggc atgcgccgcc gcggtcgcgc cgtaccgctc gggtcgatgg tggtgatgac
     7141 cgtggcgtgt gcgatcgcgg cgccgctgat cgcccgctgg tggggtgccg acgccgcgta
     7201 ctgggcgagc ccggccaggg ccggtgagat cctggtcggc gcgacccttg ccgtggctct
     7261 gcacgccgga cgcatccatc ccacccgttg gatggccccg gtcgggttgg tcggggtgat
     7321 cgcactcggc gtcgccctgc cggcgaccgg aggaccggcc taccagggct ggatgacgcc
     7381 gctcgcgctc gtcagcgggc tcttgatcgt cggcctccaa cgccccggcc cggtacggca
     7441 actcttggga tcgcggccct tcgccgtgac cggacgtgtc agctacgggt tgtacctgta
     7501 ccacttcccg gtgttcgtcg ccttggacga gcaccgtctc ggcttcggtg gcgtcggcct
     7561 gttcgtcatc cgcatcgnnn nnnnnnnnnn nnnnnnnnnn nnncacctga tcgagcaacc
     7621 ggtgcggaac agccagatgc aaccgcggcg ggcgttcgcg tccgcgttgt ccgcgaccgc
     7681 cgtcacgacc tgcctggtct ggatcgtgcc gtcggcggcg agcgccaact acttccaggt
     7741 cgatagcgag ttggcccacc aggcccagat cgaaacaccc gccgacggct cgctcgcgcc
     7801 gctcgtcgtg gcaacgacga ccaccacagc accgaccgtc agtgcgcctg cgagctcagc
     7861 tgtttcgtcc acgacgacgg tcaccgattc gccgaccgcg ccgactacgc cgacggctcc
     7921 gaccgcgacg gcgatgccga cgacaaccgt tgttccggcg acggattcgg tgccgcccac
     7981 cgtggtcggc ctgtacgacg tggtggtgac gcccgtcctc aaccgtccgg tccggatcgt
     8041 ggtgctgggc gactcgaccg cggttgccct cggcgcgggg ctggtgcgct gggccgccga
     8101 gcgtcnnnnn nnnnnnnagc actggacggg tgcgggttca ttcacggggg caccggcgcc
     8161 gacaacccct tcaccgtgcc ctgccaagag cacctcgacg tcgaactccc ggcatcgata
     8221 cccgccgcac aacccgatgt cgtggtgttg atgagtacgt tgggcgactt cggcccgcgc
     8281 acctgggacg acggtggcat cgacctgcag ccgaccgacc ccgagtacct cgtccgtctg
     8341 caagctggct acaccgcgat cagcgaactg atcgagtcga ccaccaatgc gnnnnnnnnn
     8401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntcga catgcagacg
     8521 tgggcaacaa caaccggtct cgatcagtcg caggctgctc gccccgacgg catgcacttc
     8581 gcagcacagg cggcgtacga gatctcggtc gcgtggctcg gcccccggct ggtgctggcg
     8641 gcgacgtcgg gtcgctagcc acccgacacg ccaccctgac ccgtcgccgc gtcggcctgt
     8701 gcgggtagat cgggcaccgc ctacggtggt cacccgtgtc agacccccag caactcgccg
     8761 cttccgtcga cgtcccggtc agcgcccaca gccgagtcca cacccaaggc atcgcgtcgt
     8821 gagccgcgcc gagcagttcg aactgctccc cgacgaggtc gcccagcgct ggcggaagtt
     8881 cggccggagc gagcgcatct tctacaccca gttgctcggc atcgaggtgg aagaggtgcg
     8941 ggtcgactac tgccgcatgc gcctgccgtt tcggcccgag ttgcagcaac cggcgggtct
     9001 ggtgcacggg ggagcgctgg catcgttgct cgacgccgtc gtggtgcccg cgatcggagc
     9061 cgtctacgaa gcggacgtgc gctttgccac cgtcgacatg cacgtgcagt tcatgtccgc
     9121 cgtgcgcgac cacgacgtgg tgggcgaggg gtgggtgacg cggcgcggac gcaccaccgc
     9181 gttctgcgaa gccgaagccg tcgacgccgt gaccggcgcg acggtggcgc gcagcatgct
     9241 gacgtacaac atcgccaatc cggccggttg acgtaccgat taccgacgcg gtgatacagg
     9301 gcggatcggt gcccaccgtc tacggttgag acccgtggcg acccccggca acaccctctc
     9361 cgaatccgcg tccaaggctt tgcttgccgc tcacggcgtg ccgctggcgg ccgagcgcga
     9421 agtgctcacg gccgcgcagg ccggatcgtt tccagcccgt cgttgtcgat tccgcaggcc
     9481 gccgctgcgg ccaccgagat cggcttcccg gtggtggtca agctgtgcgg tgacgcgatc
     9541 gctcacaaga ccgagcgcgg cctggtcaag ctgcgtctcg gcgatgccga ggccgtcagg
     9601 acggcgagcg aggagttgct cgccaaggcc acacccgacg acggcgatgt cagcgtgctg
     9661 gtcgcgccga tgatcgccgg caaccgcgag ttgatcgcgg gcatgctgcg cgacgcccag
     9721 ttcggcgcca cgatcatgct cggtgtcggg ggcatcctgg ccgaggcgat cgccgacgtg
     9781 gtgttccgcc ccgcaccgct cacacggctc gaagccgggg agatgatcga tggcctggca
     9841 acccaggcac tgctgcaacc gttccgtggc gaagcggcgg tgaaccgcga cgcactgatc
     9901 gacgtactgg tcggtctggg cgatatcgcc gaggcgcgtc ccgacgttgc cagcatcgac
     9961 gtcaacccgc tgatcgtgcg cgccgacggc atgccgatcg ccgtcgacgc tctggtcgag
    10021 atcgcaacgc cggcaacagc gacagccgac acccacgtcg tacccgacct ggcgctgttc
    10081 gacgcgctgt tcaacccgcg tggtgtggtg gtgacgggtg cgtcgaccca tccgggcaag
    10141 ttcggcttcg tctcgctgca caacatcctc gccagcgggt accagggcaa ggtgttcggc
    10201 acgaacctga agggcgaaga ggtgctcggc atcaccacgg tgcgcgacct cgacgaactg
    10261 cccgagggtg aggtcgacct ggccttcgtc tgtacgccag cggccaccaa ccccgatctg
    10321 ttgcgggcct gcgcggccaa gggcatccgc gcc
//
LOCUS       DAORNF010000018         9729 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_5160_length_9729_cov_1.525946, whole genome shotgun sequence.
ACCESSION   DAORNF010000018 DAORNF010000000
VERSION     DAORNF010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 9729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9729
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5160_length_9729_cov_1.525946"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="PLV68_01440"
     CDS             <1..337
                     /locus_tag="PLV68_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99927.1"
                     /translation="DLGELAPGSIELAGDDRRSRFGQPTDAHQATTRDVAPLVVPSVV
                     PPTGRMLLGRMAGRGPLLATEDRQRHPLTGRGARRQGNRGSVALIGPTGSGKTALATS
                     AIATSSPCP"
     gene            328..>726
                     /locus_tag="PLV68_01445"
     CDS             328..>726
                     /locus_tag="PLV68_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99928.1"
                     /translation="MSVKRDLYDTTVAARAAKGDIAVFDPGGVTGLPTARWSPLEATV
                     TSSGALRTGRALAQAIPKGGVTNAEYWAEHGEGLLGAFMCVAGLSRLLTDDDGRPMKP
                     VGMEQLAVWVRTLAGGSDPTINSIISRACDN"
     assembly_gap    727..765
                     /estimated_length=39
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <766..1904
                     /locus_tag="PLV68_01450"
     CDS             <766..1904
                     /locus_tag="PLV68_01450"
                     /inference="COORDINATES: protein motif:HMM:NF014581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HRD99929.1"
                     /translation="GREHAHLAVPREDDKIRSSVYSTAVLALKPWLEPAVAHSASMSP
                     RAFYSGGDLFSHRPRYIDLDWLMDGEPGRSNTLYLSAPTAEFERLSPVLGGVLADLKD
                     TIHAWDIARRHLDKPLLIVIDEAAHLQLGWLPTEVSTIAALGAFFVTCWQNLAQINHR
                     YDSLADAVLSGHRTKCFFAGIDDLTTLRYLTTMLGHEEVTRRGWSHDIPSVWGNRQGG
                     RRSVSESTQREEFAPAHTLREMIPGEAVLFHGTLPPIHLTAVRHWEEPELAALVHHDD
                     ALAEQTCPLTDDLVDDGDHPAIDIATLEAAKAQLPKPSRAAPPAAAVDPTAMPSSVAV
                     VDDTDLGNCVSCGEPLAPGAWQPHRGSTSARRCAPNCHERANRL"
     gene            complement(2148..2759)
                     /locus_tag="PLV68_01455"
     CDS             complement(2148..2759)
                     /locus_tag="PLV68_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99930.1"
                     /translation="MVQKNIARARQRHPDATPAEVLRSLERMYLAALTGQGAAVGGVA
                     AAPGVGTGAALALSGGEVLSMLELSALFALSLAEVHELERRRTLVMGILLGGSGSSMI
                     PKVAGRTAKYWGREVASRVPVATLKQINKVLGKNFVTKFGTKQGIIVIGRVAPFGIGA
                     AIGGGANALLARSAIQAGRRAFGPAPRAWSNELHEAAPPKTEA"
     gene            2781..3110
                     /locus_tag="PLV68_01460"
     CDS             2781..3110
                     /locus_tag="PLV68_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99931.1"
                     /translation="MIERYLNLLARRGRWQLGHRVVVVHYLPAPFCQLPEVKHVCIAL
                     RLAVAVKADIVRLPLVRAAGTAWSRLVDVHAAAAGSMARHEEAVVTHRLRLGADRVNI
                     RIATNKR"
     assembly_gap    3164..3229
                     /estimated_length=66
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3565..3602
                     /estimated_length=38
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3817..3848
                     /estimated_length=32
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3849..>4728
                     /locus_tag="PLV68_01465"
     CDS             <3849..>4728
                     /locus_tag="PLV68_01465"
                     /inference="COORDINATES: protein motif:HMM:NF024565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRD99932.1"
                     /translation="ARGEKERGQETARKLETDTARTISQELQAAGGRFAARSYNATKV
                     RDGLGDPTKTSTVASDDEVAADLQIVQSHKLDELTLLPSPQFDLAKIEADVAAALTQS
                     ITSDALPELTGDADAEQWVRHGINLHEHRKRCLLCNNVLDTDRRAELDRHFDESFTRL
                     QNRLESLDRALQAEDIRVSQVLSTAPDRGLLYSDLQDDYRTQLEQSGRQVTEYKKRIA
                     ALGEQVARKRATPFALIEPGDALAGHGAVDLNALNAVVEEHNMRSSDHQQVVSAAADR
                     IERARLSVIAGDHSAA"
     assembly_gap    4729..4848
                     /estimated_length=120
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4849..>5213
                     /locus_tag="PLV68_01470"
     CDS             <4849..>5213
                     /locus_tag="PLV68_01470"
                     /inference="COORDINATES: protein motif:HMM:NF024565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRD99933.1"
                     /translation="ELTRDIATLLGREELSFGKEGDRYSIQRNGEPATALSEGEQTAI
                     SLLYFLCSLRDEKTRGISATVIIDDPVSSLDHEILVGVSGHLWSALVGNGSTHQVVLL
                     THSFELFRLWSNQLDRLPGA"
     assembly_gap    5214..5232
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5289..>5628
                     /locus_tag="PLV68_01475"
     CDS             5289..>5628
                     /locus_tag="PLV68_01475"
                     /inference="COORDINATES: protein motif:HMM:NF024565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRD99934.1"
                     /translation="MRRPVMVSWSDEKLRKKLRSQYHYLFWRIGSLVIDHGVDGDLVS
                     ELEAAAIIPNAARRMLEAFLAFKYPSKLGDFDGSMRRTFEEGLVADPLRQRVTRFLHQ
                     QSHNEDADISR"
     assembly_gap    5629..5801
                     /estimated_length=173
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5802..6073
                     /locus_tag="PLV68_01480"
     CDS             <5802..6073
                     /locus_tag="PLV68_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99935.1"
                     /translation="SRERADPPIRQVLEADHRFGLVAVRRYNWNQSYSAADYRQLMLS
                     YSVTQMMDETDRVGRLDDMETFIRDRFGGDITRPLVVALTTATLM"
     gene            6214..7041
                     /locus_tag="PLV68_01485"
     CDS             6214..7041
                     /locus_tag="PLV68_01485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HRD99936.1"
                     /translation="MEQIRLEALLPVGFDPATCKLHCAVFNGDQHPIDVLASDPVGWQ
                     GWNSWRPHKDVFSRQFIFSLAQDRHDPTLWLFGGIWEVVGRRPQPHARSYDVVHREDL
                     MGAFVRRLYIRLPLAGRERRRNMESCLEQMTVSSILEEAFVGDPFPGHDRINHTLAEL
                     TVIVSQQRPDWRIALEHMKGVYVIHDQTTGARYVGSAYGDTGIWQRWSVYVATLHGSN
                     VGLIELLDERGADYFRVNMKFALLEFWSMRTADAHVLERESYWKDVLQARSLGHNKN"
     gene            7169..7309
                     /locus_tag="PLV68_01490"
     CDS             7169..7309
                     /locus_tag="PLV68_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99937.1"
                     /translation="MLEVAGLGVTWWTMPGECSEPVADKVGAAMVSGGAAVVSQSVSL
                     SG"
     assembly_gap    7434..7529
                     /estimated_length=96
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7530..8073
                     /locus_tag="PLV68_01495"
     CDS             <7530..8073
                     /locus_tag="PLV68_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99938.1"
                     /translation="VGRVSDTSDAVVAALLAMPLDDDLVAPVLVAGLRRLLFLCRGSR
                     RELLDDLVVELAIAIGELRRVRPLVFRRRLAYVIVDRARDRQRAALRRERAWRPVDAL
                     GVAELLESGDAGVEEAAVDRVRLGVVRDQIVAAGDVALARSWNSLVELMDSPRESQAE
                     RDRWKYVRRRLAGHLGPDAA"
     gene            8178..8801
                     /locus_tag="PLV68_01500"
     CDS             8178..8801
                     /locus_tag="PLV68_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99939.1"
                     /translation="MVVVAKPFRFDESDPRAMLRHLGQWARYSLGTPEAWGSALERCG
                     HWVDYSARNQLLLASYGVATPVAGAATWERVPSVEPDRGCAVRTGEHGLPVRVPVVEA
                     GEVTADRLRGAGRSDAVAGGHRWELVFAAEQLARRPAVGVLTPPSVPSLSDRDWAEAV
                     RVASGRMTGRTPRSVIRPTISDGVAVAAAVRGTSSPVAAGTLTSAPP"
     assembly_gap    8818..8833
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8869..>9729
                     /locus_tag="PLV68_01505"
     CDS             8869..>9729
                     /locus_tag="PLV68_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99940.1"
                     /translation="MTGRERWQSAVDVRRSVDQVMAAVSFAVGVELTSSPLPRHGLVD
                     DREVRAGRRNYLAPSDLRGLPLGVWVEAGPYKRGEWLARGVAGAVGVGGFMRVNDRSY
                     LAVYETKAGAMWRLETTGRGAHLGLVGQGESETFDDAKRDVAAALAERFPDAAAAIDA
                     VTTNRVVSGNFGWAKLPEGRDDRTQHRRYDEHVTAMISPGPGGRWQTWVSVDGEQRQG
                     PYAPNPTAARTVADGLARGALLELASVTPSRANEIVRDLATGDSAWNRAELVAVIGPR
                     LTDADRHELAT"
BASE COUNT         1501 a         3092 c         3042 g         1495 t
ORIGIN      
        1 tgacctcggc gaacttgcac ccggcagcat cgagttggcc ggcgacgacc gcaggagccg
       61 gttcgggcaa cccaccgacg cccaccaagc caccacccgc gacgtcgccc cgctcgtcgt
      121 cccatccgtc gtgccgccca ccggacggat gctgctcgga cgaatggcag gccgcggtcc
      181 gctgctcgcc accgaagacc gccaacgaca cccgctcacg ggtcgcggtg cacgacggca
      241 aggcaaccgc ggatcggtcg cgttgatcgg gccgaccggg tcgggcaaga ccgcgctcgc
      301 cacctccgcg atcgccacgt cgtcgccgtg tccgtgaagc gcgacctcta cgacacgacc
      361 gtcgccgccc gcgccgccaa aggcgacatc gccgtgttcg accccggcgg cgtcaccggc
      421 ctgccgaccg cccgctggtc accactcgaa gccaccgtca cctcctccgg tgcactccgc
      481 accggccggg cgctcgccca agcgatcccg aaaggcggtg tcacgaacgc cgagtactgg
      541 gccgaacacg gcgaaggact actcggcgcg ttcatgtgcg tcgccggcct ctcccgccta
      601 ctcaccgacg acgacggccg gccgatgaag ccggtcggca tggaacaact cgccgtgtgg
      661 gtccgcaccc tcgccggcgg cagcgacccg acgatcaact cgatcatcag ccgcgcctgc
      721 gacaacnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnccggg cgcgaacacg
      781 cgcatctggc cgtgccgcgc gaggacgaca agatccgctc cagcgtgtac tccacggcgg
      841 tgctggcgtt gaagccgtgg ctcgaacccg ccgtcgcgca ctcggcctcg atgagcccac
      901 gcgcgttcta ctccggcggc gacttgttca gtcaccggcc ccggtacatc gacctcgact
      961 ggctgatgga cggcgaaccc ggccgctcca acacgctcta cctgtcagca ccgaccgccg
     1021 aattcgaacg gctctcgccc gtcctcggcg gcgtgctcgc cgacctcaaa gacacgatcc
     1081 acgcctggga catcgcccga cgccacctgg acaagccgct cctgatcgtg atcgacgaag
     1141 ccgcccacct ccagctcggc tggctcccca ccgaggtctc gacgatcgcc gcgctcggag
     1201 cgttcttcgt cacctgctgg cagaacctcg cccagatcaa tcaccgctac gacagcctcg
     1261 ccgacgccgt cctgtccggg caccgcacca agtgcttctt cgccggcatc gatgacctca
     1321 ccaccctgcg ctacctcacc acgatgctcg gccacgaaga agtcacccgc cgcggttgga
     1381 gccacgacat cccctccgtg tggggcaacc gccaaggcgg gcgccgcagc gtgtccgagt
     1441 cgacccaacg cgaggagttc gcgcccgcgc acaccttgcg cgagatgatc cccggtgaag
     1501 ccgtcctgtt ccacggcacc ctcccgccga tccacctcac cgccgtccgc cactgggaag
     1561 aacccgaact cgccgccctc gtccaccacg acgacgcgct cgccgagcag acatgcccgc
     1621 tcaccgacga cctcgtcgac gacggcgacc accccgccat tgacatcgcc accctcgaag
     1681 ccgccaaagc ccaactcccc aaacccagcc gggcagcacc gcccgccgcg gcggtcgacc
     1741 cgaccgcgat gccgagcagc gtcgcggtcg tcgacgacac cgacctcggc aactgcgtct
     1801 cgtgcggcga acccctcgcc cccggcgcct ggcaacccca ccgaggctca acgagcgccc
     1861 gccgctgcgc accgaactgt cacgaacgtg cgaatcgtct ctgacgcggt gttcctgctg
     1921 cggacgtccg gcggagcagg ttgccttcgg ctgctgcgag tccggacgcc agccgtgcgc
     1981 cgtgccatca gcagtcttga ccatgggccg gcgccggtgt cgacgcctca tgcgcctgcc
     2041 cctcgtcggc acttcggtga cacctcctgt cagcggggca gatcgggacg gcggcctgcg
     2101 cggagagaca tctccagccg acctcgtgca ggagggtgat ggccagttca ggcctcggtc
     2161 ttgggtggcg cagcctcgtg aagctcgttc gaccacgctc gcggcgccgg cccgaacgcc
     2221 cgccgaccgg cctggatcgc ggaccgagcg aggagggcgt tcgctccacc gccgatggca
     2281 gccccgatgc cgaacggcgc aactcggccg atcacgatga tcccctgctt cgtgccgaac
     2341 ttggtgacga agttcttgcc gagcaccttg ttgatctgct tcaacgtggc gaccggcacg
     2401 cggctagcca cctcacgccc ccagtacttc gctgtccgcc cagcaacctt gggaatcatc
     2461 gatgagccgc tgccgccgag gaggatgccc atgaccaagg tccgccgccg ctcaagctcg
     2521 tgtacttcag caagcgagag ggcgaatagc gcgctcagtt cgagcatcga caggacctca
     2581 ccgccggaca aggcgagcgc agctccggtg ccaactcctg gcgccgcggc caccccgccg
     2641 accgcagccc cctgtcctgt gagcgccgca aggtacatcc gctccaggct gcggaggact
     2701 tcagcggggg ttgcatcggg gtggcgctga cgcgcacgag cgatgttctt ctgaaccacc
     2761 gtcccctgga tgctgagtgc atgatcgagc gctacctgaa ccttctggcc cgccgaggcc
     2821 gctggcaact tggtcaccgc gtcgtcgttg tccactacct acctgccccg ttctgtcagt
     2881 tgccggaggt gaagcacgtg tgcatcgccc tgcgccttgc tgtcgccgtg aaggccgaca
     2941 tcgtacgtct cccgctggtt agggcagccg ggacggcttg gtcgcgactg gtcgacgtac
     3001 acgcagccgc cgccggatcc atggcccgtc acgaggaagc cgttgtcact catcgcctgc
     3061 gcctgggcgc cgaccgggtc aacatccgga tcgcgacgaa caaacgatga ggactccgtg
     3121 cacgcggaga cgcttgcgga ccggctttca gccggtctgc cccnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt catccgtgaa
     3241 caggccggat tggctcagga ggatcgtctt tcccgtgccc gccgcgaccg aacagagccg
     3301 ccattgcctg cgtccgcggc gccctcggtc gtgtcgttga gcgcgagcct gcgacacaat
     3361 cgaccgtgtg gtgacgacaa gcaacccaga gtgtcgacaa ggcgaccgct cgtgatgcgc
     3421 cgcatcatca aggtcagcgg tgcaggcatc ttcgaccaat gggctccatc gcccgaggtc
     3481 gaagacttct cccgagtgaa tgtcgtctac ggagggaacg gcagcggaaa gagcaccctc
     3541 gcgcggacct tctcgcagat gaccnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nntcagcgtg gaggttctcg gagcggatgg aacgccacgc cgggtgtcgg atcgatccga
     3661 cgccttctgg tcccaagtgc gcgtcttcga caaggacttc gttaggaaga acgtgctgtt
     3721 cgatgtcggc ggtcggagtg aggcactgcc gcttctcgtc ctcggcgagc ccaacgttga
     3781 tcgtgacaag cggctggctc agatcgacga acgattnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnag gctcgcggag agaaggaacg aggccaggaa acggcacgga agctggagac
     3901 cgatacagcg cggaccatca gccaagagtt gcaggcagca ggtggccggt tcgcggcgcg
     3961 tagctataac gcgaccaagg ttcgtgatgg gctcggagac ccgacgaaga ccagcaccgt
     4021 ggcatcggat gacgaggtcg cagcggacct ccagatcgtg cagagccaca agctcgatga
     4081 actcaccctc ctcccttccc cgcagttcga cctcgccaag atcgaagccg atgtagccgc
     4141 agctctgacg cagagcatca ccagcgacgc tctcccagaa ctcaccggag acgccgatgc
     4201 cgagcagtgg gtgcggcacg gaatcaatct tcacgagcat cggaaacgct gcctcctgtg
     4261 caacaacgtt ctcgacaccg accggcgtgc tgaactcgac cgccatttcg acgagagctt
     4321 caccaggctc cagaatcgac tcgaatccct cgatcgtgcc ctccaagcgg aagacatccg
     4381 ggtcagccag gtcttgagca cggcgccgga tcggggactg ctctactccg acttgcaaga
     4441 tgactatcgg acacagcttg aacagtcggg tcggcaagtc accgagtaca agaagcgaat
     4501 cgccgcactc ggagaacagg tcgcccggaa acgagccacc ccgttcgctc tcatcgaacc
     4561 aggcgatgcc ttagcgggtc acggtgcagt ggacctgaat gcgctcaacg ccgtggtcga
     4621 ggagcacaac atgcggtcat ccgaccacca gcaagtcgtc tctgctgccg ccgatcgcat
     4681 cgagcgcgcc cgactgtcgg tgatcgccgg cgatcactca gccgctcann nnnnnnnnnn
     4741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnga actgacccgc
     4861 gacatcgcga ctctgctcgg acgtgaggag ctttccttcg gaaaggaggg tgaccgctac
     4921 tccatacagc gcaacggcga acccgccacc gcgttgagcg aaggagaaca gactgcgatt
     4981 tccctgctgt acttcctatg cagcctccgc gacgaaaaga cacgcggaat ctccgcgacg
     5041 gtgatcatcg acgatccggt gtccagcctc gaccacgaga tccttgtggg cgtctctggc
     5101 cacttgtgga gtgcgctggt tggcaacggc tccacccacc aggttgtcct gctcacccac
     5161 agcttcgaac tgttccgcct gtggagcaac caactggatc gccttcccgg agcnnnnnnn
     5221 nnnnnnnnnn nncgtactcg atctacgagc tccgcacgcg cttccaccct cgcacggatg
     5281 gcggctccgt gcggcgcccc gtgatggtgt cgtggtctga cgagaagctg cggaagaagc
     5341 ttcgatccca ataccactac ctcttctggc gcatcggcag tctcgtcatc gaccacggcg
     5401 tcgacggaga cctggtgagt gagctcgaag cagcagccat cattcccaac gcggcgcgcc
     5461 gcatgctcga agctttcctt gccttcaagt accccagcaa gctcggcgac tttgacggct
     5521 cgatgcgccg aactttcgaa gaaggattgg tggcagaccc gcttcgtcaa cgagtgacgc
     5581 gcttcctcca ccagcagtcc cacaacgaag acgccgacat cagtcggcnn nnnnnnnnnn
     5641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncgagccgcg aacgcgctga
     5821 cccgccgatc cgacaagtgc tcgaggccga ccatcgcttc ggcctcgtcg ctgtacgccg
     5881 atacaactgg aatcagagct acagcgccgc ggactatcgg caactgatgc tgtcgtactc
     5941 cgtcacacag atgatggacg aaaccgaccg tgttggtcgg ctcgacgaca tggagacctt
     6001 catccgcgac cgattcggcg gcgacatcac ccggccgctc gtggtcgccc tcacaaccgc
     6061 cacgctcatg tgaccgtcgc catctggtcg acatcgcacg tggcccacga cgccggcacg
     6121 gcgtagtgcg ggccaagtga tgtgtgcggc cgtaccgaga cctccgggcg cagtcgagcg
     6181 cgccgggttc gacctgctac gtacgatcag gtcgtggagc agattcgtct cgaagcgctt
     6241 cttcccgtgg gattcgatcc ggcgacgtgc aagctgcatt gcgcggtctt caacggcgat
     6301 caacacccga tcgacgtcct ggccagcgac ccggtgggat ggcaggggtg gaactcgtgg
     6361 cggcctcaca aggacgtctt cagccggcag ttcatcttct ccctcgctca agatcgccac
     6421 gatccgacgc tgtggttgtt cggagggatc tgggaggtcg tcggacggcg tccccagccc
     6481 cacgcccgtt cgtacgacgt cgtccatcgc gaagatctaa tgggggcgtt cgttcgccgt
     6541 ctgtacatca ggttgccgtt ggccggtcgg gagcggcgcc gaaacatgga gtcgtgtctc
     6601 gagcagatga ccgtgtcgtc gatcctcgag gaggcgttcg tcggtgaccc gttccccggc
     6661 cacgaccgca tcaatcacac gctcgccgag ctcacggtca tcgtgagcca gcagcgaccg
     6721 gactggcgga tcgcgctcga acacatgaag ggtgtctacg tcatccacga tcagacgacc
     6781 ggtgcccgct acgtcgggtc ggcgtacggc gacacgggca tctggcagcg ctggagcgtc
     6841 tacgtggcga ccctgcacgg gagcaacgtc ggtctcatcg agctcttgga cgagaggggt
     6901 gcggactact tccgggtgaa catgaagttc gctctgctcg agttctggtc gatgcgcacc
     6961 gctgacgctc acgtcttgga gcgtgagtcg tactggaagg acgtccttca ggctcggtcg
     7021 ctgggtcaca acaagaactg atctgccggg agacagtgac gccggacgtc cagcacggca
     7081 tcccttgtcg ccggcctggc cgacaacggc aacccaacaa ccgctgacct ggccacggcg
     7141 ccgggtccgc tgggcgtcat ctcgtcgcgt gctcgaagtc gccgggttgg gggtcacgtg
     7201 gtggacgatg ccgggtgagt gctccgagcc ggtagccgac aaggttggag cggcgatggt
     7261 gtccggtggt gctgccgtcg tctcgcagag cgtgtcgctg tcgggctgaa ctcgcttcgt
     7321 gccggaaagt gcggtcgatt ggatgatcgg gggacaggga gcgctccggg gagtcctgtt
     7381 tcgcatggtg cgggcatgga gctcttcgat gcactccctg gctcgctgaa tctnnnnnnn
     7441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnng gtcggtcgtg tgagcgacac gtcggacgcg
     7561 gtcgtcgcgg cgctgttggc gatgccgctg gacgacgatc tggtcgcccc ggtcttggtc
     7621 gctggtctgc ggcggttgtt gttcttgtgt cgtgggtcgc ggcgggagct gctggacgac
     7681 ctggtggtcg agttggcgat cgcgatcggt gagctgcgcc gggtgcggcc gttggtgttc
     7741 cggcgccggt tggcgtacgt gatcgttgat cgtgcgcggg atcgtcagcg ggctgcgttg
     7801 cggcgagagc gggcgtggcg tccggtggac gcgctcggcg tcgctgaact actcgagtcc
     7861 ggagacgcgg gcgtcgagga ggctgcggtg gatcgggtgc gcctgggggt cgtgcgcgat
     7921 cagatcgtgg cggcaggtga tgtggcgttg gcacggtcgt ggaactcgtt ggtggagttg
     7981 atggactccc cgcgcgagtc ccaggccgag cgagatcgtt ggaagtacgt gcggcgccgg
     8041 ttggcagggc atctcggccc ggatgctgcc tgacctgggg cggggagtcc tccagcacgg
     8101 ccggggagtc ctcccggcgg agcgagggcg ttgggtaggg agcgctccgg ggagtcctga
     8161 ggctgacggt gaccggcatg gtggttgtcg ccaagccgtt caggttcgac gagtccgatc
     8221 cgcgggcgat gctgcggcat ctgggtcagt gggcccgcta ctcgctgggc acgccggagg
     8281 cgtgggggtc ggcgttggag cggtgcgggc attgggtcga ctactcggca cgcaatcagc
     8341 tgctgttggc ctcgtacggg gtggcgactc cagtggcggg cgcggcgacg tgggagcggg
     8401 tgccgtcggt ggagcccgat cgcggctgcg cggtgcgtac cggtgagcac ggtctgccgg
     8461 tgcgggtgcc ggttgtcgag gcgggtgagg tgacggccga ccggttgcgc ggggcgggcc
     8521 ggtcggatgc ggtggcgggt gggcatcggt gggagttggt gttcgctgcg gaacagttgg
     8581 cgcgacggcc agcggtcggt gtgctcacgc ccccatcggt gccgtcgttg tcggatcggg
     8641 actgggccga ggcggtgcgg gtggcgtcgg gccggatgac ggggcgcacg ccgcggtcgg
     8701 tgatcaggcc gacgatctcc gacggcgtcg ccgtcgcggc ggcggtacgc ggcaccagct
     8761 cgccagttgc cgccggaacg ttgacctcag cgccaccctg aatcgcctgc agcgcgtnnn
     8821 nnnnnnnnnn nnnccgaggg cccgatgccg gggttcgatc cgtcaggatt gacgggccgg
     8881 gagcggtggc agtcggcggt ggacgttcgt cgttcggtgg atcaggtgat ggcggcggtg
     8941 tcgttcgcgg tcggggtcga gttgacgtcg tcgccgctgc cgcgccacgg tctggtcgac
     9001 gaccgtgagg tgcgtgcggg gcgcaggaac tatctggccc cgtcggatct gcgtgggttg
     9061 ccgttgggcg tgtgggtcga ggccggcccg tacaagcgcg gcgaatggtt ggcgcgcggt
     9121 gtcgctggcg cggtcggcgt gggcgggttc atgcgggtca acgatcgttc gtatctcgcc
     9181 gtgtacgaga ccaaggcggg cgcgatgtgg cggctcgaga cgaccggccg cggcgcgcat
     9241 ctcgggctgg tcggccaggg cgagtcggag acgttcgacg atgccaaacg tgacgtcgcg
     9301 gcggcactcg ccgaacgatt cccggacgcc gccgcggcga tcgacgcggt caccaccaac
     9361 cgggtcgtgt cgggcaactt cggctgggcg aagctgcccg agggacgcga cgaccgcaca
     9421 cagcaccgcc gctacgacga acacgtcacg gcgatgatct cccccggccc cggtggtcgg
     9481 tggcagacgt gggtgagcgt cgacggcgag caacgccaag gcccctacgc ccccaacccg
     9541 acggcggcgc gcacggtcgc cgacggcctc gcccgcgggg cgttgctgga gttggcatcg
     9601 gtgacaccgt cccgggcgaa cgagatcgtc cgggacctcg ccaccggtga cagcgcctgg
     9661 aaccgggccg agctggtcgc cgtgatcggg ccccggctca ccgacgccga ccgccatgaa
     9721 ctcgccacc
//
LOCUS       DAORNF010000019         9609 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_5240_length_9609_cov_1.768369, whole genome shotgun sequence.
ACCESSION   DAORNF010000019 DAORNF010000000
VERSION     DAORNF010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 9609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9609
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5240_length_9609_cov_1.768369"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="PLV68_01510"
     CDS             complement(<1..213)
                     /locus_tag="PLV68_01510"
                     /inference="COORDINATES: protein motif:HMM:NF015909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactose-lipid carrier transferase"
                     /protein_id="HRD99941.1"
                     /translation="MARLDRLDLSQKLTAAEYSKRIALAQRRLLQLRLHLGGQMGSGE
                     IGPALLIVLEGPDAAGKGGAIKRVTER"
     gene            complement(272..>1434)
                     /locus_tag="PLV68_01515"
     CDS             complement(272..>1434)
                     /locus_tag="PLV68_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99942.1"
                     /translation="TATIPLGVEIGGFPAPLAAGRVVVKASGSGSGSDELLIVDIASA
                     RVERVAIPETVTPFGTAPTDVRVVVADGGRGSEALMIDAGSGRLTDLRATLGIEHLFF
                     AGRRGDSLLFQGLNGSDVSTVVVPMASSTQPLQIDGSVIDVRGDRILAVDRTRETMRV
                     FERDRLVASYPAPVGLIGGALSVDAASGAVSVLAVSEDGAVTLLADGASTSLGVGGGG
                     GIAGVLRVGDDRVWLTGADSQLSLLLSLRGEVIETFAPVGGVALQPWNQGLRDAQQCV
                     VVQPGPQPLARDGVMVVSLRDGSIVAPLSSSGQVLDTVDGCTAVPTGGPSTTAVVAGK
                     VVTLPEEASIVAVSPDGSTLVYRVQRRVVAWTIASGKTFELDSGSFYCYVLS"
     assembly_gap    1435..1540
                     /estimated_length=106
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2318..2521)
                     /locus_tag="PLV68_01520"
     CDS             complement(2318..2521)
                     /locus_tag="PLV68_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99943.1"
                     /translation="MKVAPTIKRRTVTAKEILGDVAAWKAAHPGFDESNFPDAFRDAN
                     GELIESDEFFAAAQLFATFRMLH"
     gene            complement(2618..3136)
                     /locus_tag="PLV68_01525"
     CDS             complement(2618..3136)
                     /locus_tag="PLV68_01525"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRD99944.1"
                     /translation="MEIRWLEGGDVDACVGLNNASIPAVSEIDVAGLQRLVDQSAASL
                     VVVDDDPHHGRQDDVVGFCIVLGPGVDYGSVNYRWFSERYDDFAYLDRIVVADHYRNN
                     GAGAALYGEVERRLAAVAPWLLCEVNLRPRNDGSLRFHARLGFTEVGQQDTNYGITVS
                     MLAKPLAPTTAR"
     gene            3165..>4013
                     /locus_tag="PLV68_01530"
     CDS             3165..>4013
                     /locus_tag="PLV68_01530"
                     /inference="COORDINATES: protein motif:HMM:NF012708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HRD99945.1"
                     /translation="MSYELRMANGGTVELDAAVVIGRGPAEPGVRMIIVDDPLVSKAH
                     LDIDVAGGVLRVRDRGSSNGSVLRQGGVDVALTPHAWTAVGPDAALVVGETVLTVVPR
                     AAAPGTVIAGHGPSGSVGQAPPDVLDMTIPTGSAGLGGRGYNPGPTPASTPPTSPAPA
                     GLGPAPGALLSPGLHTPPTTPGNGRKRLVIALVAVVVIAALVVGGIAVFGGDDSSSAS
                     AEQLPRVVTERPKEQWAVDVDGSIGGVAADGSRVFIATAANDRARVQAVARDTGDEVW
                     NARFGRT"
     assembly_gap    4014..4178
                     /estimated_length=165
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4179..>4458
                     /locus_tag="PLV68_01535"
     CDS             <4179..>4458
                     /locus_tag="PLV68_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99946.1"
                     /translation="NVIVSKDNSAEAVAITTGESIARVRGDTVRIRDDGYVYVFRKDE
                     VEVFDARSFRTLFGPVEIDDDLFGLRYDGRHLVVLVDDELQFIDASGKE"
     assembly_gap    4459..4636
                     /estimated_length=178
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4637..4920
                     /locus_tag="PLV68_01540"
     CDS             <4637..4920
                     /locus_tag="PLV68_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99947.1"
                     /translation="TDAGTLVAIDDDGAYVVVDQRSGKQVADFDADFVRFGTNAIVAY
                     EFRTNGGFDVAVYDLKTGKELWSDDYAQAAIVVDGGVIVYQDDEIAYYK"
     assembly_gap    5014..5035
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5036..5718)
                     /locus_tag="PLV68_01545"
     CDS             complement(<5036..5718)
                     /locus_tag="PLV68_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HRD99948.1"
                     /translation="MTTTVNTAATGSAGTVRIAAIQHDIAWGDRDANFAHTAPFIATA
                     AAGGARLALLTETFSTGFVTDRPVGEPEGGPSSQFIADQAARHGMWVGGTCPADAGDD
                     QRPYNTFVLASPDGVLHRYRKIHPFTYGGEDKHFRAGGSFATVEVEGLRVTLFVCYDL
                     RFADEFWQLARETDVYLVPANWPESRRVHWQALLQARAIENQAYVVGCNRVGEGGGLA
                     YSGDSRIIDP"
     gene            complement(5715..>6017)
                     /locus_tag="PLV68_01550"
     CDS             complement(5715..>6017)
                     /locus_tag="PLV68_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRD99949.1"
                     /translation="ALADLAAKRDRLCAGLVDAGFEVYRPQATYFVTVDIRGLRPDGD
                     GYAFCRDLPHRCGVVAIPNEVFYTDARRGKHLVRFAFSKRFEVLDEAVARLKELNA"
     assembly_gap    6018..6027
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6028..>6318)
                     /locus_tag="PLV68_01555"
     CDS             complement(<6028..>6318)
                     /locus_tag="PLV68_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRD99950.1"
                     /translation="IEHDLIVVTDEVYEHLLFDGLRHTPIATLPGMASRTLTISSGGK
                     TFNTTGWKVGWVVGPAPLVAAVCTAKQYLTYVHSGPFQPAIAAGLRMPDEFFV"
     assembly_gap    6319..6402
                     /estimated_length=84
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6403..>6702)
                     /locus_tag="PLV68_01560"
     CDS             complement(<6403..>6702)
                     /locus_tag="PLV68_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRD99951.1"
                     /translation="PPLIGVPALRHAVAEHQQRFYGMTVDPDTEVLATCGASEALAAT
                     LLALLDTGDEIVAFEPSYDMYGACAAMAGAVLRPVTLHPPDYAVDLDAVRAAITPR"
     assembly_gap    6703..6790
                     /estimated_length=88
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7038..7108
                     /estimated_length=71
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7109..7322)
                     /locus_tag="PLV68_01565"
     CDS             complement(<7109..7322)
                     /locus_tag="PLV68_01565"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF004064.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HRD99952.1"
                     /translation="MFRAALAVQAHAYAPYSNYRVGAALLTPSGIYAGCNVENAAYPQ
                     GACAEAGAIAAMCAAGERSILAVLTVC"
     gene            7438..8535
                     /locus_tag="PLV68_01570"
     CDS             7438..8535
                     /locus_tag="PLV68_01570"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HRD99953.1"
                     /translation="MRIAISTGGGDAPGLNAVIRAATLAAIRRGWEVLGIRDGFNGLL
                     TPDKYVMSGGGIYHLNRDDVRGIGHLGGTILGSTNTGSPLEYPMQQPDGTWVTADRTD
                     ELVGMFHERGIDALISVGGDGSLRIANHLHQAGLRVIGVPKTIDNDLDKTTATFGFDT
                     AVEFATDCIDRVISTATSHNRVMVVEVMGRYAGWIALHAGIAGAAHAILIPEIPYDLG
                     KVAEMIRHREERQANFSIVVVAEGAAPIGGQRSVIGKQIGQAERLGGIGHIVAQQLGE
                     LTGKETRTVVLGHLLRGGSPTSGDRLLGTRFGTAAVRALEEGQDGVMVALNPPVVDYV
                     PLAEATATMKTVDPTCDTVVAARDLGISFGD"
     gene            complement(8568..9476)
                     /locus_tag="PLV68_01575"
     CDS             complement(8568..9476)
                     /locus_tag="PLV68_01575"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRD99954.1"
                     /translation="MHLPDEFEFLADNAAEVGMSLAPDVAVRRCGLDLADGQHVSGVR
                     WGTGPVELVYLHGGAQNAHTWDSVALALGRPAIAIDLPGHGYSNGEGFDRLDIAQQAL
                     TMVTVLEHVVPAPVAVIGMSLGGLVALAVSATRADLVPRLVLVDILPSSSNHRTARMN
                     SFLDGKDAFDSFEEMVEWSVLHNPGRSAAELRRGVFHNAQRGADGRWTWRHTRDVPGV
                     ARIQEPEFRGATASLWPAVASFGGPLMLAYGGTDASVLREEHVQMLRERQPAARVERV
                     QGAGHSVQRSRPRELAALIADFLGDA"
BASE COUNT         1548 a         3019 c         2990 g         1328 t
ORIGIN      
        1 acgctcggtc acgcgtttaa tcgctccacc cttgcccgcc gcatccggcc cctccaacac
       61 gatcagcagc gcgggaccga tctccccgct gcccatttgg ccgccgaggt gcaggcgaag
      121 ttgcagcagc cgccgctggg caagcgcgat tcgcttgctg tactccgccg ccgtgagctt
      181 ttggctgagg tcgagtcgat caaggcgcgc cacaaggatc catcctagaa acagtccccc
      241 acccgacccc cgacccaaat gccttcgtcc gtcaggacag gacgtagcaa tagaagctgc
      301 ccgagtcgag ttcgaacgtc ttgccggatg cgatcgtcca agccacgaca cgccgctgca
      361 cgcggtacac cagggtcgaa ccgtccggcg acaccgcaac gatcgaggcc tcctccggca
      421 acgtcaccac cttgcctgcc acgactgcag ttgtcgaggg gccgccggtt ggcacggcgg
      481 tacagccgtc cacggtgtcg agcacctgac cggaactgct caagggggcg acgatcgaac
      541 cgtcccgcag cgacaccacc atcaccccgt cacgggccaa cggttgcgga ccaggctgca
      601 cgacgacgca ctgttgtgcg tcacggagac cttggttcca cggctgcaac gcgacgccac
      661 cgaccggcgc gaacgtctcg atcacttcgc cacgcagcga caacagcagc gacagctggc
      721 tgtcggcgcc cgtgagccac actcgatcgt caccaacccg caggactccg gcgatgccac
      781 cgccgccgcc aacgccgagt gacgtcgacg cgccgtctgc caacagcgtg accgcgccat
      841 cctcactcac cgcgagcacc gacacggcac ccgatgccgc atcgacactg agggcaccac
      901 cgatcagacc gacaggtgcc ggatacgacg cgacaagtcg gtcgcgctcg aacaccctca
      961 tcgtctcccg ggtgcggtcg accgccagga tgcggtcgcc gcggacgtcg atgacgctgc
     1021 cgtcgatctg caggggttgc gttgacgagg ccatcggcac caccacggtg gacacgtcgg
     1081 aaccgttgag gccctggaag agcagcgagt cgccgcggcg gccggcgaag aacagatgct
     1141 cgatgcccaa cgtggccctg aggtccgtca gccgtccact gccggcgtcg atcatcaacg
     1201 cttcgctgcc gcgcccaccg tcggcgacga cgacccgtac atcggtcggt gctgtgccga
     1261 acggggtcac ggtctcggga atcgcaacgc gctcgacgcg tgcgctcgcg atgtcgacga
     1321 tcagaagctc gtccgagccc gagcccgacc ccgatgcctt gaccacgacc cgaccggctg
     1381 ccaacggagc ggggaaccca ccgatctcca cgccgagggg gatcgtcgcc gtcgnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cgagtcgccc gcatcgtccc
     1561 caccacggat gaccaccacc gccaccacag caaccaccgc gacaaccacg gcggccgcaa
     1621 tggcgaccac cgtccggcgc tgccttccgt gcgatggacc aggcagctct gagccaggcg
     1681 gcagcgacgg cgaagccagg ggcggagggg gcgggggcgg gggcgccatg ccgctgggca
     1741 gcgacggagg cgcgaacggg ccgggcgcgg tagggcggtc gggatgattc gtcatggcgg
     1801 tggctcctcg gtcgacgtcg cgaacaactc gcgatcaccg cgacagccca accgccccga
     1861 acagtaccgc cctccgaatg cgccccgttc agcttcccgc ctacgtcgtc cgaaactcgc
     1921 cacggaccgt cgcgtgcacc aactcgatca cttgcagaag tgtgaccgac gggtgcgcgt
     1981 gacgaacgtc ttggcgcgga ccgacatgaa gatgagtccg gccaccatgc tcgagttcgt
     2041 ggcccatgtg gttgtcgtga cgccacacga gttccgcacc acggttcagg ttgaatctat
     2101 aaaagcgagc taggagactg gcatcgaatg tgagcgcaac tgtaagcgtg aaggcgccca
     2161 gcaactcgat cgtgaacggc aggacgttcg cgcccccgtt gggctgaccg acgtactcga
     2221 tgccgctcga ctgctcaccg ccagcagcgg cgcgaggtca gtcaagtagt cgagcgcgtc
     2281 atcgaccgac ttgatgtagc cgggaaacat ggaaacgtca gtggagcatg cggaaggtcg
     2341 cgaacaactg cgcagcagcg aagaactcgt cgctctcgat cagctcgccg ttcgcgtcgc
     2401 ggaacgcgtc cggaaagttc gactcgtcga acccagggtg cgcggccttc caggcggcca
     2461 cgtcgccgag aatctctttt gccgtcaccg tccgacgctt gatcgtgggc gccaccttca
     2521 tagctgtcga tcgtaccgcc ccacgcgacg agcactcggt agggtcgggt ctctggcgat
     2581 gtggtactcg acgccagtcg accggctctg actgaggtca gcgggcggtt gtgggagcga
     2641 ggggtttggc gagcatgctg acggtgatgc cgtagttcgt gtcttgctgg ccgacttcgg
     2701 tgaaaccgag gcgggcgtgg aagcgcaacg aaccgtcgtt gcgggggcgc aggttgacct
     2761 cgcacaacaa ccacggtgcg accgcggcga gacggcgctc gacctctcca tacagcgcgg
     2821 caccagcgcc gttgttgcgg tagtgatcgg ccacgacgat gcggtcgaga taggcgaagt
     2881 cgtcgtagcg ctcgctgaac caccgatagt tgacgctgcc gtagtcgacg ccgggcccga
     2941 gcacgatgca gaacccgacc acgtcatcct gccggccatg atgcggatcg tcgtcgacca
     3001 ccaccagcga cgccgccgac tgatcgacca gccgctggag accggcaacg tcgatctcgc
     3061 tcaccgcggg tatcgacgcg ttgttgagcc ccacacacgc atccacgtca ccaccctcca
     3121 accatcgaat ctccaccgcg gcagcctagg tacgctgacg ccccatgtcg tatgagttgc
     3181 ggatggcaaa tgggggcacg gtcgagcttg atgcggcggt cgtgatcggg cggggtccgg
     3241 cagagccggg tgtacggatg atcatcgtgg atgatccgct cgtctccaag gcgcacctcg
     3301 acatcgacgt ggcaggcggc gtgctgcgcg tgcgtgatcg cgggtcgtcc aatggatcgg
     3361 tgctgcgcca gggcggcgtc gatgtggcac tgacaccgca cgcatggacc gccgtcgggc
     3421 cagacgcggc gctggtcgtc ggtgagaccg tcctcaccgt cgtcccccgc gccgccgcac
     3481 ccggcacggt cattgcgggc cacgggccga gcggcagcgt gggtcaggcg ccgccagacg
     3541 tactggacat gacgattcca acggggagtg ccggattggg aggacggggg tacaaccccg
     3601 gaccgacgcc ggcgagcaca cctccgacct cgccagcgcc ggctgggctc ggcccagcac
     3661 cgggcgcctt gctgagcccc ggcctgcaca ctccgcccac cacgccgggc aacggccgca
     3721 aacgacttgt gatcgctctc gtggcagtgg tcgtcatcgc cgcgctggtg gtcggcggga
     3781 tcgctgtctt cggcggcgac gacagctcgt cggccagcgc cgaacagctc ccccgcgtgg
     3841 tgaccgagcg gccgaaggag cagtgggccg tcgatgtcga cggttccatc ggtggcgtcg
     3901 ccgcggacgg ctcacgcgtg ttcatcgcca ccgctgccaa cgaccgggca cgcgtgcagg
     3961 cggtcgcccg cgacacgggc gacgaggtgt ggaacgcgcg tttcggcaga accnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnaa cgtcatcgtc tccaaggaca
     4201 actcagccga agcggtcgcc atcacgaccg gcgagtccat cgccagggtg cgcggtgaca
     4261 ccgtgcgcat ccgcgacgac gggtacgtct acgtgttccg caaggacgag gtcgaggtgt
     4321 tcgacgcgag gtcgttccgc acgctgttcg gccccgtcga gatcgacgac gacctcttcg
     4381 gcctgcggta cgacggcaga cacctggtgg tgctggtcga cgacgagctg caattcatcg
     4441 acgcgtccgg caaggaacnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4621 nnnnnnnnnn nnnnnncgac cgacgccggc acgctcgtcg ccatcgacga cgacggggcc
     4681 tacgtcgtcg tcgatcagcg cagcggcaag caggtcgccg atttcgacgc ggacttcgtt
     4741 cggttcggga ccaacgccat cgtcgcctac gagttccgca cgaatggcgg gttcgacgtc
     4801 gcggtctacg acctcaagac cggcaaggag ttgtggtcgg acgattatgc gcaggccgcg
     4861 atcgtggtcg acggcggcgt gatcgtgtac caggacgacg agatcgccta ctacaagtga
     4921 gccctggcct acgagcggcg gtcgcgctgg aaggggaagc gggcgcggac gtcggcgacg
     4981 accgcaacag tgtctcgcag cgacttttcg tcgnnnnnnn nnnnnnnnnn nnnnnggatc
     5041 gatgatccgg ctgtcaccgc tgtaggccaa acccccacct tcaccgacgc ggttgcaacc
     5101 gacgacgtag gcctggttct cgatcgccct cgcctggagc agcgcctgcc agtgcacgcg
     5161 gcgcgattcg ggccagttgg cgggcaccag atacacgtcc gtctcgcgcg cgagttgcca
     5221 gaactcgtcg gcaaagcgga ggtcgtagca gacgaacagc gtgacccgca gcccctcgac
     5281 ctcgaccgtc gcgaacgagc caccggcacg gaagtgcttg tcctcgccgc cgtaggtgaa
     5341 cgggtggatc ttgcgatagc ggtgcagcac gccgtcgggc gacgcgagca cgaacgtgtt
     5401 gtacgggcgc tggtcgtcgc ccgcgtcggc gggacacgtc ccaccgaccc acatgccgtg
     5461 gcgggcggcc tggtcggcga tgaactgcga cgagggacca ccttcgggct caccgacggg
     5521 gcgatcggtg acgaagcccg tgctgaacgt ctccgtcaac agtgcgaggc gtgccccgcc
     5581 cgccgcggcg gtggcgatga acggtgcggt gtgggcgaag ttggcgtcgc gatcgcccca
     5641 tgcgatgtcg tgctggatcg ccgcaatgcg gacggtgcca gcagaaccgg tcgcggcggt
     5701 gttcacagtt gtcgtcatgc gttgagctcc ttcagtcggg cgacggcttc gtcgagcact
     5761 tcgaaccgct tgctgaatgc gaaccgcacc aagtgcttgc cgcggcgggc atcggtgtag
     5821 aagacctcgt tgggaatggc caccacaccg caacggtgcg gcaagtcgcg acagaacgcg
     5881 taaccgtcac cgtccgggcg caggccgcgg atgtcgacag tgacgaagta ggtcgcctgc
     5941 gggcgataca cctcgaaccc ggcgtcgacc agccccgcac agaggcggtc gcgcttggcg
     6001 gccaggtcgg cgagcgcnnn nnnnnnngac gaagaactcg tcaggcatcc gcaacccggc
     6061 cgcgatcgcc ggttggaacg ggccgctgtg gacgtacgtg aggtactgct tggcggtgca
     6121 cacggcagcg acgagcggcg ccggaccgac gacccagccc accttccagc ccgtggtgtt
     6181 gaacgtcttg ccgccactgg aaatggtcag cgtgcgtgac gccatgcccg gcagcgtcgc
     6241 gatcggggtg tgccgcaagc cgtcgaacaa caggtgctcg tagacctcgt cggtgaccac
     6301 gatcaggtcg tgctcgatnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncgcggggt gatcgcggcg
     6421 cgcaccgcgt cgaggtcgac cgcgtagtcg ggcgggtgca acgtgacggg gcgcagcact
     6481 gcgccggcca tcgccgcgca ggcgccgtac atgtcgtagc tgggttcgaa ggcgacgatc
     6541 tcgtcgccgg tgtcgagcag cgccagcagc gtggccgcca acgcttcgct ggcaccgcac
     6601 gtggcgagca cctcggtgtc ggggtcgacg gtcatgccgt agaaccgctg ttgatgttcg
     6661 gcaacggcat gacgcagcgc gggaacaccg atcagcggcg ggnnnnnnnn nnnnnnnnnn
     6721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6781 nnnnnnnnnn tggccgaggt tgaccgagcc ggtcgccact gccaatgccg acatctcgcc
     6841 gaaaatcgtg gtgcccaggc cttgcaggcg gctggacagg aacggttggc ggctggcgtt
     6901 catgggcgtg actccgatcc ggcttctggc atgtcgagat gggcggggcc gaagctgtgc
     6961 ggcagcagcc cgccgagagt gaacgtggcc cgcacgccgg aggcatcggc ggcgtacacg
     7021 gggatgtcga gtgacgcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7081 nnnnnnnnnn nnnnnnnnnn nnnnnnnncg cacacggtga gcacggcgag gatcgatcgc
     7141 tcgccggccg cgcacatcgc ggcgatcgct ccggcctcgg cgcaggcccc ttgcgggtag
     7201 gcggcgttct cgacgttgca gcccgcgtag atgccgctgg gggtgagcag ggctgcgccg
     7261 acccgatagt tcgagtaagg cgcgtaggcg tgagcttgca ccgccagcgc agcgcggaac
     7321 atggcctcga cctggcttgc tccagcgcct tcgtcggtac ctgccggtgc gacgcccgga
     7381 tgaagagacg atgaatcggg acgaggtgcc acgcccgcga cgctaccgtt gcccccgatg
     7441 cggatcgcga tcagtactgg aggcggcgac gcgcccggcc tcaacgccgt cattcgggct
     7501 gccacactgg cggccatacg ccgtgggtgg gaggtgctcg gcatccgcga cggcttcaac
     7561 gggttgctca cgcccgacaa gtacgtcatg agcggtgggg gcatctacca cctgaaccgc
     7621 gacgacgtcc gtggcatcgg gcatctgggc ggcaccatcc tcggcagcac caacaccggg
     7681 agtccgctgg agtatccgat gcaacaaccg gacggcacgt gggtcacggc cgaccgcacc
     7741 gacgagctcg tcggtatgtt ccacgaacgg ggcatcgacg cgttgatctc agtgggcggc
     7801 gacggctcgt tgcgcatcgc caaccacctg caccaggcgg gcctgcgggt gatcggtgtg
     7861 cccaagacga tcgacaacga cctcgacaag accacggcca cgttcggctt cgacaccgcg
     7921 gtcgagttcg ccaccgactg catcgatcgg gtgatctcca ccgcgacctc gcacaaccgg
     7981 gtgatggtgg tcgaggtgat gggtcgctat gcgggttgga tcgcgctgca cgccggtatc
     8041 gctggcgccg cgcatgcgat cctcatcccc gagatcccct acgacctggg caaggtggcc
     8101 gagatgattc gccaccgcga ggaacgtcag gccaacttct cgatcgtggt cgtcgccgag
     8161 ggcgcggcac ccatcggtgg gcagcgctcc gtgatcggca agcagatcgg ccaggccgaa
     8221 cgcctcggcg gcatcggcca catcgtcgcc cagcagttgg gcgagctgac cggcaaggag
     8281 acgcgcacgg tggtgctggg tcacctgttg cgcggcggct cgccgacgtc gggcgatcgt
     8341 ctgctcggca cccgcttcgg taccgccgcg gtgcgtgcgc tggaagaggg ccaagacggc
     8401 gtgatggtgg cgctcaaccc tcccgtggtc gactacgtgc ctctggccga agccaccgcc
     8461 acgatgaaga cggtcgatcc cacctgcgac accgtcgtcg ccgcacgaga cctgggcatc
     8521 agcttcggtg actaggttcg gcgactggct tcgctgatcg gcaccgatca ggcgtcgccg
     8581 aggaagtccg cgatcagcgc ggcgagctcg cgcggtcgcg accgctgcac gctgtgaccc
     8641 gcgccttgca cccgctcgac gcgcgctgca ggctggcgct cgcgcagcat ctgcacgtgc
     8701 tcttcacgca gcaccgacgc atccgtccca ccgtaggcaa gcatcagcgg tccaccgaag
     8761 ctggcgacgg cgggccacag cgatgcggtc gcgccacgaa actcgggctc ttgaatccgt
     8821 gccacccccg gcacgtcgcg cgtgtgccgc cacgtccaac gcccgtccgc accgcgctgc
     8881 gcgttgtgga acacaccccg gcgtaattcg gccgcagaac gaccggggtt gtggagcaca
     8941 ctccactcga ccatctcctc gaagctgtcg aaagcatcct tgccgtcgag aaacgagttc
     9001 atcctcgcgg tgcgatggtt gctggaggag ggcaggatgt cgaccagcac gaggcgcggg
     9061 acaagatcgg cacgggtcgc cgacactgcg agcgcgacga gcccaccgag cgacatgccg
     9121 atcaccgcaa cgggcgcggg taccacgtgc tccaacaccg tgaccatcgt gagcgcctgc
     9181 tgggcgatgt cgaggcgatc gaacccttcg ccgttcgagt agccatggcc cggaagatcg
     9241 atggcgatgg cagggcgccc gagggcgagc gcgaccgagt cccacgtgtg cgcgttctgc
     9301 gcgccgccgt gcaggtagac cagttcgacc gggccggttc cccagcgcac accgctcacg
     9361 tgctggccgt cggccaggtc cagcccacat cggcgcactg cgacatcggg ggctagcgac
     9421 atcccgacct cggccgcatt gtcggcgagg aactcgaact cgtcgggaag atgcatgtca
     9481 gggttggtca caactcacct ttcagcgcgc gggatgggcc accagcacgc aatccgtaga
     9541 cagcggcgat caccgctgtc atgccagcca acaacacgcc agaacgcgcg ccgacgtgct
     9601 cgctcagcc
//
LOCUS       DAORNF010000020         9536 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_5297_length_9536_cov_1.427803, whole genome shotgun sequence.
ACCESSION   DAORNF010000020 DAORNF010000000
VERSION     DAORNF010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 9536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9536
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5297_length_9536_cov_1.427803"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            208..>878
                     /locus_tag="PLV68_01580"
     CDS             208..>878
                     /locus_tag="PLV68_01580"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRD99955.1"
                     /translation="MKRPFVLFLIVLLVGGGLYARRGCSAQRAAVAPDAPAPVAPSAG
                     AMLKAQREILELPLEREADPVGELPLSGFVVDDTGAPVPEAAVTIDTVPERQVASASD
                     GGFEVLGLAPRNYVLTAQRGALVSLPTTVQLGESTAPVTLQLRRVAGLEISVVDGERN
                     LPIAGAQVEVRGAGLRRSGTTAADGSVALLGVAPGSYTLFAAAAGYAPTYLRHAVMDA
                     LGGSSR"
     assembly_gap    879..935
                     /estimated_length=57
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            945..>1329
                     /locus_tag="PLV68_01585"
     CDS             945..>1329
                     /locus_tag="PLV68_01585"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRD99956.1"
                     /translation="MAAESASELVSLVDPARDGVVTDERGQWRIAALTPGSYRFVARK
                     AGQAPGGTPPTQVDARGARDITITLPAAARLAGLVTDTSGAPVPFATVRAVVDEGAWG
                     HAVARQVTCSASGEFELAGLPRKLVN"
     assembly_gap    1330..1464
                     /estimated_length=135
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1465..2677
                     /locus_tag="PLV68_01590"
     CDS             <1465..2677
                     /locus_tag="PLV68_01590"
                     /inference="COORDINATES: protein motif:HMM:TIGR03317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99957.1"
                     /translation="ARAQVWAEPSAPSARTRIEGTLRGALSAVAGEDGSFAFYGLPTG
                     RYSLRASWPGTAAKARTVALRPPVVAETGARDVRLVLVADGGVRGTVQRNDGVAPAMF
                     TVALGGGAAFGGGSGEFAITGVPAGKHTLRISGLDFATRTIDGLEVKPGQLLDLGRIT
                     VVRGRQLTGKVVRADGSPVAGATVTVTNTGAATLSLLGATVGDGRFTIEASTVEVIAV
                     RNAPGVLAAPDAANAVPAWGRDDAFDLFPPAQPPEGIERGDSATLTLARIEAGWPATG
                     AELTEATIPAETGFVVGLAVSFTKGCYPGQELVERMDSRGSSAPRTIRRLRGSGSAAV
                     GDPVVGADGKIVGHLSSVAATADGRGDGWVALVPLARSVEVDADGAATTTLTVGGLPA
                     VVEPANVNTVP"
     gene            2677..3513
                     /locus_tag="PLV68_01595"
     CDS             2677..3513
                     /locus_tag="PLV68_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99958.1"
                     /translation="MAGATTRLTNATLATGELVDVTISAGDRDTAGAGRIVAVDPAAG
                     AQRSPGDRHDEHVEHDDLTVELAGHLLTTSLVEPHAHLDKAFLAERIPNPTGDLIGAI
                     HGMHANRDLITVADTIERAERAVRLMVNNGVTLIRTHADVTDANGLTSVEALAEVRRR
                     TAGLCDLQIVALPGFPLTGPAGAPTRALLREALDTGADLVGGCPHLEDDVDGATEVLL
                     AIAAEFGRGVDLHTDETLNPSKLGLADLARRCSSPAANSTFSRPRAISPAASGPAAFP
                     TR"
     gene            3423..3995
                     /locus_tag="PLV68_01600"
     CDS             3423..3995
                     /locus_tag="PLV68_01600"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRD99959.1"
                     /translation="MQQPGGELHVLEAARDLARRVRASGFPHPVTASHCVSLSMQSES
                     DQQRIAEAVAEAGVSIVALPQTNLFLQGRDHQQAMPRGLTAVRALSRAGVNVCAGADN
                     LQDPFNPIGRGDPLETAGLMIAVGHLLPDEAWSAVTSAARLALLGQRASIAPGQPADL
                     IALPVATVREAIAFTPGQRIVFRNGHRLSP"
     gene            4094..4864
                     /locus_tag="PLV68_01605"
     CDS             4094..4864
                     /locus_tag="PLV68_01605"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRD99960.1"
                     /translation="MVSTSTGTPRLAGRRAVITGSARGIGAEIAATYRREGADLVLID
                     LDGDRLHDTASDLGAKAIVVDLSDAAATAAAVSEAIGVLGGVDILVNNAGILQMAPIL
                     EMDVDTWDRAFDVNVRSMFVTTQVAGRVMRDQPLLGTRRGTIVNMASMGGKLGGPNQA
                     HYAASKAAVISFTRVTAMEFGPHAVTANCICPGYVLTEMGAATRTDEMVRTWSAKSPL
                     GRLAQTTDVANMALFLASDEADYCTGQAMNVSGGMVMH"
     gene            4864..5652
                     /locus_tag="PLV68_01610"
     CDS             4864..5652
                     /locus_tag="PLV68_01610"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRD99961.1"
                     /translation="MTDTSSPPAGIASNDVAANPPALRFNGVSMVFPDGTHALNATSF
                     DVRPGEFVTVVGPSGCGKSTLLKIASGLNKPTAGTVSVDRSRLGYVFQDATLLQWRNV
                     LRNVELLAELEKMPKGERRDKAIEAIRLVGLAGFEKKYPKQLSGGMKMRASLARSLVL
                     DPSVFLFDEPFGAVDEITREYLNDEVIKLFLLNGFAGLFITHSISEAVFLSTRVLVMS
                     ARPGTIVANFDVPFAYPRSPELRFEPAFAELSGQISHALRGAHA"
     gene            5649..>5966
                     /locus_tag="PLV68_01615"
     CDS             5649..>5966
                     /locus_tag="PLV68_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99962.1"
                     /translation="MNTLATDASDTTTMVASPPAEALELVGPRKRPFKISRVVGPVVV
                     FALFIGIWYFMHYKGLRLFFDKDGERLIPLPHDIWDATIVSKITREDYIAGIGWTAVA
                     AYGG"
     assembly_gap    5967..6094
                     /estimated_length=128
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6095..>6387
                     /locus_tag="PLV68_01620"
     CDS             <6095..>6387
                     /locus_tag="PLV68_01620"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRD99963.1"
                     /translation="IIYVVFGGSMTARLFVCVMISFFPIVTNALFGMKSVERSQHDLF
                     TLRDASRWTRLIKLQLPSALPNIFTGFRISAGLSIIGAVVGEQFFRGGSKPGVG"
     assembly_gap    6388..6458
                     /estimated_length=71
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6627..>7319
                     /locus_tag="PLV68_01625"
     CDS             6627..>7319
                     /locus_tag="PLV68_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99964.1"
                     /translation="MKHRRSTVIAASAITTSLLLAACGSDKKAAETTTTTAAPSTTEA
                     PSTTEAPSTDAVSLAGKCPDLVTLQTDWMPEAEHGFLYQMIGAGYTMDADKAYVTGPL
                     IDQNGNDTGVQLQIRSGGAPQQFQSVSTIMYDNDDVFLGYVYTDEGIQNSSTKPTVAV
                     LSGFDKNPQMIMWDPATYPEVKEIGDLKATGAVVRYFGGAAYMDYFTSTGLLDPKQVD
                     GSYQGGSDLFIAD"
     assembly_gap    7320..7329
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7330..7846
                     /locus_tag="PLV68_01630"
     CDS             <7330..7846
                     /locus_tag="PLV68_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99965.1"
                     /translation="GKAAQQGFGSAEPWNYENEYEGWKKPVAYSYINDAGWENYAESI
                     ATKPENITKYADCFKGLVPIMQHALVDYLNSPDRANAIILDAVNTFGDDFGWAYTAGT
                     LAYGVKTMKDDKLVANGPDGVMGKFDLERVANLIEKAIPVYTAQDSPPKEGLKPEDFV
                     TNEFLDPSIGL"
     gene            7944..>8400
                     /locus_tag="PLV68_01635"
     CDS             7944..>8400
                     /locus_tag="PLV68_01635"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HRD99966.1"
                     /translation="MANPHRIGKYLAERALGAGSFATVWLAFDELLEVHVAIKVLADN
                     WARNADIRRRFVEEARLLRRIDHDRVVRVYDVDELPDGRPYFVMAVADKQTLHERIEE
                     YGRGVPFDTAEALRLGIEICEALSIVHDFGVVHRDIKPSNILFRSIKNHE"
     assembly_gap    8401..8444
                     /estimated_length=44
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8445..>8754
                     /locus_tag="PLV68_01640"
     CDS             <8445..>8754
                     /locus_tag="PLV68_01640"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99967.1"
                     /translation="AGRVLGDAAVMIGDFGLAKDLAVASGFTLAAGTPAYMAPEQANP
                     TAVIDRRVDVFATAAVIYELLAGRPAFKSDTLSGVRRSRAESAVQPLIEVRPDLPQGL
                     S"
     assembly_gap    8755..8834
                     /estimated_length=80
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8897..>9536
                     /locus_tag="PLV68_01645"
     CDS             8897..>9536
                     /locus_tag="PLV68_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99968.1"
                     /translation="MVDLPGSSMPPPPAVVRAPVRLAVTGAAGRVNDLLTGSRLLTAD
                     DPVTIAATERLARPFTAVFVGAANPAVASVVASVMPRDDLVVRHVGTPDDDAVASADV
                     VVVVVGAGEPDPAAVLTALSARLKAAEAGPYAIEAVVVGADAAACAATRDAVLATPVG
                     QTLVPQIAIADQSALAALRSFVTTLADIRGIAFLESADSELVTLGEELRRWRL"
BASE COUNT         1449 a         3193 c         3009 g         1360 t
ORIGIN      
        1 cctcggccag ccaggtcgac tacgtggttc acctcgagtg gagcgactac gtggttcacc
       61 tcgagtggcg cgacatcgcg gaggctgccg ccaacgccgg gatggcggcg gacagcctgt
      121 tcgacaagta agcggaaggc cgtagcggtc tccacgagga tcgagcttgc ggccgcagct
      181 cggtcctcat catctttttc gggaatcatg aagcgaccat tcgttctctt tctcatcgtg
      241 ctgctggtcg gcggtgggct ctatgctcgt cgtggctgct ccgcgcagcg cgcggcggta
      301 gcgccggacg cgccggcacc cgtggcgcct tcggcgggag ccatgctcaa ggcccagcga
      361 gagatcctcg agttgccgct ggagcgcgaa gccgatccgg taggggagct gccgctgtcc
      421 ggcttcgtcg tcgatgacac gggcgcaccg gtgcctgagg cggcggtgac gatcgacacc
      481 gtgcccgaac ggcaggtggc cagcgccagc gatggtgggt tcgaggtgct tggcctggcg
      541 ccgcgcaact acgtgctgac cgcgcagcgc ggtgcgctgg tgtcgctgcc aaccacggtg
      601 caactcgggg aatcaacggc gccggtcact ctgcagctcc ggcgagtcgc cggcctggag
      661 atctccgtcg tcgatggtga gcgcaacctg cccatcgcag gggcccaggt ggaggtgcgc
      721 ggcgccggcc tcagacgctc cggcacgacc gcggccgatg gttcggtggc gctcctgggc
      781 gtggccccgg gcagctacac gctctttgcg gcggctgccg gctacgcacc cacgtacctt
      841 cgacacgcgg tcatggatgc gctgggaggt agtagccgnn nnnnnnnnnn nnnnnnnnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnggagc ccaggtggcg gcggagagcg
      961 cctccgagct ggtctcgctc gtcgatccgg cgcgcgatgg agtggtcacc gacgagcgcg
     1021 gtcagtggcg gatcgccgcg ctcacccccg gcagctaccg gttcgtcgcc cgcaaggcag
     1081 ggcaagcgcc cgggggaacg ccgcccacgc aggtcgatgc gcgcggcgcc cgcgacatca
     1141 ccatcacgtt gcccgcggcg gcgcgcctgg ccggtctggt cacggatacc agtggggcgc
     1201 cggtgccgtt tgccacggtg cgcgcggtgg tcgatgaggg agcctggggc cacgcggtgg
     1261 cgcggcaggt gacgtgctcg gccagcggcg agttcgagct ggccgggttg ccgcgcaagc
     1321 tggtgaacgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnncgcgcg agctcaggtg tgggccgagc cgagcgctcc
     1501 gagcgctcgc actcgcatcg agggcacctt gcgtggggcg ctgtccgccg tcgctggcga
     1561 ggacgggagc ttcgcgttct acggcttgcc gaccggccgc tacagcctgc gcgcgagctg
     1621 gccggggacc gcagcgaagg ctcgcaccgt cgcgttgcgg ccgccggtgg tggccgagac
     1681 tggcgcacgc gacgtgcggt tggtgctggt ggccgatggc ggggtgcgcg gcaccgttca
     1741 gcgaaacgat ggggtcgcgc ctgccatgtt cacggtggcg ctcggcggcg gcgcggcgtt
     1801 cggcggcggc tcgggcgagt tcgcgatcac cggggtgccg gcgggtaagc acacgttgcg
     1861 catcagcggc ctcgatttcg ccacgcgaac gatcgatggc ctcgaggtga agccgggaca
     1921 gctgctcgat ctcggccgca tcaccgtggt gcgcgggcgt cagctcaccg gcaaggtggt
     1981 gcgcgccgat ggtagcccgg tagcaggcgc caccgtgacc gtcaccaaca ccggcgccgc
     2041 cacgctgagc ctgctgggcg ccaccgtcgg cgacggccgc ttcacgatcg aggccagcac
     2101 cgtcgaggtg atcgccgtgc gcaacgcacc aggggtgctc gctgcgcccg atgctgccaa
     2161 cgctgtgccg gcatggggac gggacgacgc gttcgatctg tttccacccg cgcagccacc
     2221 ggaaggcatc gagcgcggtg actcggccac gctgaccctg gcgcgcatcg aggcgggttg
     2281 gccggccacg ggtgccgaac tgaccgaggc gaccatcccg gcggagaccg gcttcgtcgt
     2341 cggcctcgca gtcagcttca ccaagggctg ctaccccggc caggagctgg tcgagcggat
     2401 ggacagccgc ggctcatcgg ccccgcgcac catccgtcgc ctacgcggct cggggtccgc
     2461 cgccgtgggc gaccccgttg tcggcgccga cggaaagatc gtcggtcatc tgagcagcgt
     2521 cgccgcgacc gcggatggcc ggggagatgg atgggtggcg ctcgttcccc tggcccgctc
     2581 cgtggaggtc gacgcagacg gcgctgcgac cacgaccctc accgtcggtg gtctgcccgc
     2641 cgtcgtggag ccggccaacg tgaacacggt gccgtgatgg cgggtgccac gacgcggctc
     2701 accaacgcca cgctggccac gggcgaactg gtcgacgtca cgatcagcgc cggcgaccgc
     2761 gacactgcgg gcgcgggacg gatcgtcgcg gtcgacccgg cggccggtgc gcagcgctcg
     2821 cccggcgacc gccacgatga acacgtcgag cacgacgatc tgacggtcga gctcgccggg
     2881 cacctgttga cgaccagtct ggtcgagccc catgcccacc tcgacaaggc cttcctcgcc
     2941 gaacggatcc cgaatccgac cggcgacctg atcggcgcga tccacggcat gcacgccaac
     3001 cgcgacctga tcaccgtggc cgacacgatc gagcgagccg aacgcgcggt gcggctgatg
     3061 gtgaacaacg gggtcacact gatccgcacc cacgccgacg tcaccgacgc caacgggctg
     3121 acctcggtcg aggcactggc cgaggtccgc cgccgtacgg caggcctgtg cgacctgcag
     3181 atcgtcgccc tacctggctt cccgctcacc ggcccagccg gggcacccac gcgcgcgctg
     3241 ctgcgcgagg cactcgatac cggtgccgac ctggtgggcg gctgcccaca tctcgaagac
     3301 gacgtcgacg gcgcgaccga ggtgctgctg gcgatcgcgg ccgagttcgg ccgcggggtc
     3361 gatctgcaca ccgacgagac cttgaacccg tccaagctcg gcctggccga tctcgcccgc
     3421 aggtgcagca gcccggcggc gaactccacg ttctcgaggc cgcgcgcgat ctcgcccgcc
     3481 gcgtccgggc cagcggcttt ccccacccgg taacggccag ccactgcgtg tcgttgtcga
     3541 tgcagtccga atccgaccag cagcgcatcg ccgaagcggt cgcggaggcg ggggtcagca
     3601 tcgtcgccct gccacagacc aacctgttcc tgcagggccg cgatcaccaa caggcgatgc
     3661 cccgcggact caccgccgtg cgcgcgctga gccgggccgg tgtcaacgtc tgcgccggag
     3721 ccgacaacct gcaggacccg ttcaacccga tcggacgtgg cgacccgctg gagaccgccg
     3781 ggctgatgat cgccgtcggc cacctgctgc ccgacgaagc atggtccgcc gtgacgagtg
     3841 cagcccgcct cgccctgctc ggccagcgag catcgatcgc tcccggtcag cccgccgacc
     3901 tgatcgccct cccggtcgcc accgtccgcg aagcgatcgc gttcaccccc ggccagcgga
     3961 tcgtgttccg caacggccac cgcctcagcc cctgaccacc gtgggcagac cgtgtgaacg
     4021 gcgtgtgaat gaagaccacc tcgacagcgc cggatcaggg ccgccagagc cggtcgccta
     4081 agatggcgcc cgcatggtca gcacctccac gggaacacct cgcctcgccg gccggcgggc
     4141 ggtcatcacc gggtctgcgc gcggtatcgg ggccgagatc gcagccacct accggcgcga
     4201 gggcgctgat ctggtgctga tcgacctcga cggcgaccgg ctgcacgaca ccgcgtccga
     4261 tctcggcgcc aaggcgatcg tggtcgatct gtccgacgca gcagcgacag ccgcggccgt
     4321 cagcgaggcg atcggcgtcc tcggcggggt cgacatcctg gtcaacaacg cgggcatcct
     4381 gcagatggcc cccatcctcg agatggacgt cgatacgtgg gaccgagcct tcgacgtcaa
     4441 cgtgaggtcg atgttcgtca ccacccaagt ggccgggcgg gtgatgcgcg accagccgct
     4501 gctcggcaca cgacgaggca ccatcgtcaa catggccagc atgggcggca agctcggcgg
     4561 gccgaaccag gcgcactacg ccgcgtccaa ggcggcggtc atctccttca cccgcgtcac
     4621 ggcgatggag ttcggccccc acgcggtgac cgccaactgc atctgccccg gctacgtgct
     4681 gaccgagatg ggcgcagcca cgcgcaccga cgagatggtg cgcacatggt cggccaaatc
     4741 acccctcggt cgattggcgc aaacgaccga cgtcgcgaac atggcactgt tcttggcaag
     4801 cgacgaggcc gactactgca ccggccaagc gatgaacgtc tccggcggga tggtgatgca
     4861 ctgatgaccg atacgagctc acccccggcg ggcatcgcca gcaacgacgt ggcggcgaac
     4921 ccgccggccc tccgcttcaa cggcgtgtcg atggtgttcc ccgacggcac ccacgccctc
     4981 aacgccacgt ccttcgacgt ccgcccgggc gagttcgtca ccgtcgtcgg cccgtcgggc
     5041 tgcggcaaga gcacgctgct gaagatcgcc tcaggcctca acaagcccac ggcgggcacc
     5101 gtatcggtcg atcgcagccg cctcggctac gtgttccagg acgcgacgct gctgcagtgg
     5161 cgcaacgtgc tgcgcaacgt ggagctactg gccgagctgg aaaagatgcc caagggtgag
     5221 cggcgcgaca aggcgatcga ggcgatccgc ctggtcggcc tggccgggtt cgagaagaag
     5281 tatcccaagc agctctccgg cggcatgaag atgcgtgctt cgttggctcg ctcgctggtg
     5341 ctcgacccgt cggtgttcct gttcgacgag ccgttcggcg ccgtcgacga gatcacccgc
     5401 gagtacctca acgacgaggt gatcaagctg ttcctgctca acgggttcgc cgggctgttc
     5461 atcacccact cgatcagcga ggcggtgttc ctgtcgaccc gcgtcctcgt gatgagcgcc
     5521 aggcccggca cgatcgtggc caacttcgac gtgcccttcg cctatccccg ctcccccgag
     5581 ctgcgcttcg agcctgcctt cgccgaactg agcggccaga tctcccacgc gctccgaggt
     5641 gctcacgcat gaacacgctc gccaccgatg ccagcgacac gaccacgatg gtggcgagcc
     5701 cacccgccga agcgctcgag ctggtcgggc cgcgcaagcg cccgttcaag atcagccgcg
     5761 tcgtcggtcc ggtcgtggtg ttcgcgctgt tcatcggcat ctggtacttc atgcactaca
     5821 agggtctgcg cctgttcttc gacaaggacg gcgagcgcct gatcccgctg ccgcacgaca
     5881 tctgggacgc gaccatcgtc agcaagatca cccgcgagga ctacatcgcc ggcatcggct
     5941 ggacggccgt cgcggcctac ggcgggnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnatcatc tacgtggtgt tcggcggcag
     6121 catgaccgcc cgcctgttcg tgtgcgtgat gatctcgttc ttcccgatcg tgaccaacgc
     6181 cctgttcggt atgaagtcgg tcgagcgcag ccaacacgat ctgttcacac tgcgcgacgc
     6241 ctcgcgctgg acccgtctga tcaagctgca actcccctcc gcgctgccca acatcttcac
     6301 cgggttccgc atctcggccg gcctgtcgat catcggcgcc gtcgtcggtg agcagttctt
     6361 ccgcggcggc agcaagcccg gcgtgggnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnga tgggcatcgc caccttcctg
     6481 gtcttcggcg tgctgcagcg cctcgccgtc ggccactggc acgagagcga ctccaacccc
     6541 tcgtagccgt cagttcccca tccaccccga gagcgcttgc tctcccgacc cagcgtgaac
     6601 acatcaacaa gggagaccca ccccacatga agcaccgcag aagcacagtc atcgccgctt
     6661 ccgccatcac gaccagcttg ttgctggccg cgtgcggcag cgacaagaag gccgccgaga
     6721 ccaccacgac gaccgcggcc ccgtcgacca ccgaagcacc atcgacgacc gaagcaccga
     6781 gcaccgacgc cgtcagcctg gccgggaagt gccccgactt ggtaaccctc cagaccgact
     6841 ggatgcccga agccgaacac ggcttcctgt accagatgat cggtgcgggc tacacgatgg
     6901 atgccgacaa ggcgtacgtc accggcccgc tgatcgacca gaacggcaac gacacgggtg
     6961 tccagttgca gatccgctcg ggtggcgcgc cccagcagtt ccaaagcgtc agcacgatca
     7021 tgtacgacaa cgacgacgtg ttcctcggct acgtctacac cgacgagggc atccagaact
     7081 cgtccaccaa gccgacggtc gccgtgctgt ccgggttcga caagaacccg cagatgatca
     7141 tgtgggaccc ggcgacgtac cccgaggtga aggagatcgg cgacctcaag gccaccggtg
     7201 ccgtggtccg ctacttcggt ggcgcggcct acatggacta cttcaccagc accggcctgc
     7261 tcgaccccaa gcaggtcgac ggctcgtacc agggcggtag cgacctgttc atcgccgacn
     7321 nnnnnnnnna ggcaaggctg ctcagcaggg cttcggctcg gccgagccgt ggaactacga
     7381 gaacgagtac gaaggctgga agaagccggt cgcctactcg tacatcaacg acgccggctg
     7441 ggagaactac gccgagtcga tcgcgaccaa gccggagaac atcaccaagt acgccgactg
     7501 cttcaagggc ctggtgccga tcatgcagca cgccttggtc gactacctca acagccccga
     7561 ccgggccaac gcgatcatcc tcgacgcggt gaacaccttc ggtgacgact tcggttgggc
     7621 ctataccgcg ggcaccctcg cctatggcgt caagacgatg aaggacgaca agctggtcgc
     7681 caacggcccc gacggcgtca tgggcaagtt cgacctggag cgcgtcgcca acctgatcga
     7741 gaaggcgatc ccggtgtaca ccgcccagga cagcccgccg aaggaaggcc tgaagcccga
     7801 ggacttcgtc accaacgagt tcctcgaccc ctcgatcggt ctctgatcaa gcattccggt
     7861 ctctgaccgt ttcatctgaa actatcccgt gacgaggggg cgatggctca gccgtcgccc
     7921 cctcgttcca ttagattcgc tccgtggcca acccgcaccg gatcggcaag tacctggcag
     7981 agcgtgcctt gggcgccggc agctttgcga cggtgtggtt ggccttcgac gagctcttgg
     8041 aagtacacgt cgcgatcaag gtgctggccg acaactgggc tcgcaacgcc gacatccgcc
     8101 gccgcttcgt cgaagaagcg cggctgttgc gccgcatcga tcacgaccgc gtcgtgcgcg
     8161 tctacgacgt ggacgaactg cccgacggac ggccgtactt cgtcatggcc gtggccgaca
     8221 agcaaacgct gcacgagcgg atcgaagagt atggccgcgg cgttccgttc gacacggccg
     8281 aggccctgcg cttgggcatc gagatctgcg aagcgctcag catcgtccac gacttcggcg
     8341 tggtccaccg cgacatcaag ccgagcaaca tcctgttccg ctcgatcaag aaccacgaac
     8401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncgccgg gcgggtgctc
     8461 ggcgacgcgg cggtgatgat cggtgacttc ggtctggcca aggacctcgc cgttgccagc
     8521 ggcttcacgc tcgccgcggg aacgcccgcg tacatggccc cggaacaggc caacccgaca
     8581 gcggtgatcg accgccgtgt cgacgtcttc gcgaccgcgg cggtgatcta cgaactgctc
     8641 gccggacggc ccgccttcaa gtcggacacg ctgtcgggtg tgcgccgcag ccgggccgaa
     8701 tcggcggtgc agccgctgat cgaagtgcgc cccgacctac cccaaggtct cagcnnnnnn
     8761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8821 nnnnnnnnnn nnnntcatgc cgcaaccgac ggtggtcgtc caaccggagc cgccgccagc
     8881 gcccagcaac gcgacgttgg tcgacctccc cgggagctcg atgcctccgc caccggccgt
     8941 cgtgcgcgcc ccggtgcggc ttgcggtcac cggggctgca ggacgggtca acgatctgct
     9001 caccggttcg cgcctgctca ccgcggacga tccggtgacc atcgctgcaa ccgagcggtt
     9061 ggcgcgtccg ttcaccgccg tgttcgtcgg cgcggccaac ccggcggttg cgagcgtggt
     9121 cgcgtcggtg atgccgcgcg acgacttggt cgtgcgccac gtgggcacgc ccgacgacga
     9181 tgccgtcgcc agcgcggatg tggtcgtcgt cgtagtcggc gcaggcgagc ccgacccggc
     9241 cgctgtgctg acggcgctga gcgcgcggtt gaaggctgcc gaagccggcc cgtacgcgat
     9301 tgaagccgtg gtcgtcggtg ccgacgccgc tgcctgcgcg gcgacgcgcg acgccgtgct
     9361 cgccaccccg gtcggccaga cgttggtgcc gcagatcgcg attgccgacc agtcagcact
     9421 cgccgccctg cgatcgttcg tcaccacgct ggcggacatt cgcggcatcg ccttcctcga
     9481 gtccgccgac agcgagctcg tgaccctcgg cgaggaactg cggcgctggc gtctca
//
LOCUS       DAORNF010000021         9463 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_5356_length_9463_cov_1.649979, whole genome shotgun sequence.
ACCESSION   DAORNF010000021 DAORNF010000000
VERSION     DAORNF010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 9463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9463
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5356_length_9463_cov_1.649979"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    419..428
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <429..829
                     /locus_tag="PLV68_01650"
     CDS             <429..829
                     /locus_tag="PLV68_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99969.1"
                     /translation="EIVSNAGHDAFLLEDEVMKMADPDDQPPNGFSFPLHFTWTLPPL
                     PHAPDLLILATELAGIGGIDLPLEVSAIDSFASVTDAAERSLSIVARLSIELADVYVN
                     AEEMLCDVLDRCKDVSLFLLERAPTWLDED"
     gene            917..1213
                     /locus_tag="PLV68_01655"
     CDS             917..1213
                     /locus_tag="PLV68_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99970.1"
                     /translation="MRVRELIDFLREQSPDAEVEMAIVAPVSDDSDEITVDRFSIDVM
                     VPEDRVRTLVDDDSGDEALIWLVGGEEDDIDWFLEAADELDHDHDDDDHDHPHH"
     gene            1222..1692
                     /locus_tag="PLV68_01660"
     CDS             1222..1692
                     /locus_tag="PLV68_01660"
                     /inference="COORDINATES: protein motif:HMM:NF013995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HRD99971.1"
                     /translation="MAYWLIKSEPDAFSIDDLKAVKSEIWDGIRNYQARNYLMSASVG
                     DLAFFYHSNAKPPGIVGLAKFVKVGVVDPSQFDPTSKYFDPTSKPDAPRWHTVSVKFV
                     EKFRAELSLDTLKETFSGDELMVVRRGMRLSVTPVADDVAGRILGMAGSKHAPA"
     gene            1719..2123
                     /locus_tag="PLV68_01665"
     CDS             1719..2123
                     /locus_tag="PLV68_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99972.1"
                     /translation="MSDHPAEPTPTLGRIRFAVLVQHAIEHSGLFSVLGAGWTRVDAS
                     TLPALTTINVVAQAEAVPDNGVIITEVVGPDDVVLARSETIVVVRDGPPPVTTALNLS
                     FPVELRAAGRHIVRFGGSVEAHGIDFAVGVKR"
     gene            complement(2177..>2528)
                     /locus_tag="PLV68_01670"
     CDS             complement(2177..>2528)
                     /locus_tag="PLV68_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99973.1"
                     /translation="RLVLRQVRSLDPGLGHIGDFARDLEAPRDVVMRAASIVRVDCPA
                     TATPEGTLERASWTEVLTDPVVQPDMGFAKRPFVVGTARANTDGCKRTILYQSPRQAL
                     LDVIASGSAFVVVG"
     assembly_gap    2529..2575
                     /estimated_length=47
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2576..3155)
                     /locus_tag="PLV68_01675"
     CDS             complement(<2576..3155)
                     /locus_tag="PLV68_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99974.1"
                     /translation="MEDIEGFNGAGLYTSYTGYLDPTALPDKDDHDKTIERAILRAWG
                     SYWSFEAFEERRLAGIDHLSGAMGLTVHARFDDELELNNGVVTYTATPGGQPGDADVV
                     VNVQDGDVDVTNPDPEQIELPEIINVRVRSGVVSIERQASSTLVPDGAVVLTDTAVRA
                     LIDQVGAVADTWRTRINAALSPDQQVQTFVLDF"
     assembly_gap    3172..3293
                     /estimated_length=122
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3294..>3870)
                     /locus_tag="PLV68_01680"
     CDS             complement(<3294..>3870)
                     /locus_tag="PLV68_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99975.1"
                     /translation="VGITDDPGLRQAARVRAHAIVVATESGVTVALISKAQYDEWIAS
                     QQRAPSAVTPVDISRMGHVVNLTDLVRQLGTDGAISEAEVESWRPIIGGKSAGFITLL
                     ATPGLTPPPDPLAITVRPYIEHIAGMRALIARVIADPEVLADPRARWLVLEGPDDYAG
                     QFPTAADAEYAADLVARRGGVSAVGAFVEAGG"
     assembly_gap    3871..3882
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3883..>4248)
                     /locus_tag="PLV68_01685"
     CDS             complement(<3883..>4248)
                     /locus_tag="PLV68_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99976.1"
                     /translation="GQFFARLAPILPGEVVDRLQWVIRSPQQEAVAQQMVAEGLAWSD
                     RIVHFKDLVPSGTESVYSPGVTAGRLLYVGPDGAAMAESTEHDIIVTERVPTWLPPAS
                     ALLTSDPQTPLAHVNLLARN"
     assembly_gap    4249..4274
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4275..4808)
                     /locus_tag="PLV68_01690"
     CDS             complement(<4275..4808)
                     /locus_tag="PLV68_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99977.1"
                     /translation="MVTVPVPTYIDERGFDDHSVPRVDTTAELLALARGGVGGQSAIK
                     FTIPAFDQPPDAPGLPRAHIMDSRFFGLHDEWFYFRLLNGQPVAGSGQTPVAQLQFRN
                     VEQIYAWARAQPKGSLPLGLTWAGDRLYSPRFYDLSLNTNPRTLGVGSIVHFDAADAT
                     NTTGNGTDAWLLELEYSD"
     gene            5111..5183
                     /locus_tag="PLV68_01695"
     tRNA            5111..5183
                     /locus_tag="PLV68_01695"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5144..5146,aa:Ile,seq:cat)
     gene            5251..>5970
                     /locus_tag="PLV68_01700"
     CDS             5251..>5970
                     /locus_tag="PLV68_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99978.1"
                     /translation="MSIEQLPSGKYRAVVRHGGAKRASEAVPTKAEARMLEAKLKLEM
                     GSQPSIREQHSVGEVVSGYLADGAVRLSPATLDFYRKGHDAMPDAFKQRPVTTVTPLV
                     LDSLYAELRAGGASEHKCQKVHRLLSASFNRAVRYGWLAANPCLQAAKPKVATREIEP
                     PTPEQVRELISAAAEVNEDLVVCLRLAAATGARRGELVALTWSDFVGERLTIRRSVVE
                     SDGRLIERRTKTGSKGHRTIAV"
     assembly_gap    5971..6066
                     /estimated_length=96
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6067..>6320
                     /locus_tag="PLV68_01705"
     CDS             <6067..>6320
                     /locus_tag="PLV68_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRD99979.1"
                     /translation="VYVFSFDAGVTPWRPDYLSMAFGRLSKRKVRLHDLRHYHATQLL
                     SAGVPVATVSKRLGHTSTAVTLNTYSHWLPEQDREAADVM"
     assembly_gap    6321..6347
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6348..6898)
                     /locus_tag="PLV68_01710"
     CDS             complement(<6348..6898)
                     /locus_tag="PLV68_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99980.1"
                     /translation="MLLDGGQPNDRTAFPLHEARTNRKGNPRQLVIEPGITDARIMAA
                     VEAMQPFSEANYGHDPATDALGIVGRLNNIDKHRLLLPVAHMIDHDMPAYWGSNEGDP
                     VPTYSFNSGALAAGDVVATFDFGDRLPPARFDPHIALTVTIDELTANWARGLGVVDVL
                     DGLRRGVAREINLHIVPLLNEKLL"
     assembly_gap    7170..7249
                     /estimated_length=80
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7499..7795
                     /locus_tag="PLV68_01715"
     CDS             7499..7795
                     /locus_tag="PLV68_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HRD99981.1"
                     /translation="MATTSLAELRERRDEIIAVARARGASHVRVFGSVARGDATEASD
                     VDFLVDLEPGRSLMDLGGLLMDLQDLLQRNVDVVTERGLRPRVAQRVLADAVEL"
     gene            7792..8133
                     /locus_tag="PLV68_01720"
     CDS             7792..8133
                     /locus_tag="PLV68_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HRD99982.1"
                     /translation="MRGDRDRILDMLEMCELLMEHAADERKLADDPVTQAAAQRWIEV
                     IGEAASHVSDELKLAHPDVAWREISGIRVILAHGYFHIEDDIIGNVVAGDIPTLRSQL
                     QAILASLPEPS"
     gene            complement(8249..8818)
                     /locus_tag="PLV68_01725"
     CDS             complement(8249..8818)
                     /locus_tag="PLV68_01725"
                     /inference="COORDINATES: protein motif:HMM:NF020906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1990 domain-containing protein"
                     /protein_id="HRD99983.1"
                     /translation="MPSADPPHASNRRRAALDGRGVTTGGNDLLPRRVGGRNNAPPGY
                     RLDRWDRALGAGGDVFDGAARALREWEVHRGAGLMVCADGPPAVGQVVAMSAPLPLGF
                     IDVTCRVVSVVDEPNRFGFAYGTLPIHPEQGEESFTIERDDDGEVTFRIVAVSRPRHP
                     LARLCPPAARRLQRAATVRYLDAMAGSVR"
BASE COUNT         1495 a         3015 c         3051 g         1482 t
ORIGIN      
        1 gactgcgtcg acggccaggt cgacccggta ttgaccggcg acgaaacccg acggacccca
       61 gtcggtggtg gcagccagcg tctcggcagc ggcatcggca acggcgacga acagcgacag
      121 agcctcgtct ggttgcacgg ccagcagggt agcctcgtcg cgcgctcgcc ggaccgagtc
      181 cggccgaaac accagccgac cggccgacgg gtctcactcg ccgggcgggc cgaccgaaag
      241 gggtacgtgt ggcagtcatc gatgtcgccg gcttcatcgc cgatgtcaag ggccacgcca
      301 tcgaccacgg gttccacgtc cacgacgagc gtcacttcgt cgagacctac tccctgcgcc
      361 aactgtggga ggtcgacctg caccccgagg aggcgtgcgg cggcccgctc gacctgcann
      421 nnnnnnnncc gagatcgtct ccaacgctgg ccacgatgcc ttcctcctcg aagacgaggt
      481 gatgaagatg gccgaccccg acgatcaacc gccgaacggc ttctcgtttc cgctgcactt
      541 cacctggacg ctgccgccgc tcccccacgc ccccgatctg ttgatcttgg caaccgaact
      601 ggccggtatc ggaggcatcg atctgccgct ggaggtgtcg gcgatcgact cctttgccag
      661 cgtcaccgac gccgccgagc gcagcctgtc gatcgtcgcc cgactcagca tcgaactcgc
      721 cgacgtgtac gtcaacgcgg aagagatgct gtgcgacgtg ctcgatcggt gcaaagacgt
      781 cagcttgttc ctgctcgagc gggcgccgac ctggctcgac gaggactgag cacgctccgc
      841 cgaaggtttg ggttgccgga ctcggcgcgc ccgctaaggt caccacacca gccaacgacc
      901 aaaggggcaa cacgtcatgc gcgttcgcga actcatcgac ttcttgcgtg aacaatcgcc
      961 tgacgccgaa gtcgagatgg ccatcgtcgc tccagtcagc gacgacagcg acgagatcac
     1021 ggtcgaccgc ttttcgatcg acgtcatggt gcccgaagac cgggtccgca ccctggtcga
     1081 cgacgacagc ggtgacgagg ccctcatctg gctcgtcggc ggcgaagaag acgacatcga
     1141 ctggttcttg gaagcggccg acgagctcga ccacgatcac gacgatgacg accacgatca
     1201 cccccaccac tgagaacggc tgtggcgtac tggctgatca agtctgaacc cgacgcgttc
     1261 agcatcgacg acctcaaagc cgtcaagtcc gagatctggg acggcatccg caactaccag
     1321 gcccgcaact atctgatgtc ggcctcggtc ggagatctgg cgttcttcta ccactccaac
     1381 gccaagccac cgggcatcgt cgggctggcc aagttcgtca aagtcggcgt cgtcgacccc
     1441 agccagttcg atcccacctc caagtacttc gaccccacca gcaaaccaga tgcaccgcgg
     1501 tggcacaccg tatcggtcaa gttcgtcgag aagttccgcg ccgagttgtc gctcgacacg
     1561 ctcaaggaga cgttctccgg cgacgagctg atggtggtcc gccgcggcat gcggctgtcg
     1621 gtcacgccgg tcgccgacga cgtcgccggg cgcatcctcg gcatggctgg cagcaagcac
     1681 gctccggcgt gatgccggca ctcgcctagg gtggcgccgt gagcgaccat cccgccgagc
     1741 ccacaccgac gctgggccgc attcgattcg ccgtgctggt gcagcacgcg atcgagcact
     1801 caggcttgtt ctctgtgctc ggcgccggct ggacgcgggt cgacgcctcg acgttgcccg
     1861 cgctgaccac gatcaacgtg gtggcccagg ccgaagcagt tcccgacaac ggggtgatca
     1921 tcaccgaggt cgtcgggccg gacgacgtcg tgctggcccg ctcggagacg atcgtggtcg
     1981 tgcgcgacgg tcccccaccg gtgaccaccg cgctgaacct gtcgttcccg gtcgaattgc
     2041 gcgccgctgg tcgccacatc gtgcgcttcg gtggctcggt cgaggcgcac ggcatcgact
     2101 tcgcggtggg cgtcaagcgc tgactggtgc ggtcccgctt cgcgaccgtc acggtcgtcg
     2161 aggccggagc ggccgctcag ccgacgacga cgaacgcgct gcccgacgcg atcacgtcga
     2221 gcagcgcttg gcgcggcgac tggtacagga tcgtgcgctt gcagccgtcg gtgttcgccc
     2281 gcgcggtgcc gacgacgaac ggccgcttgg cgaagcccat gtcgggctgc accaccgggt
     2341 cggtcagcac ctcggtccac gatgcccgct ccagcgtccc ctccggcgtc gcggtcgccg
     2401 ggcaatcgac ccgaacgatc gacgcggccc gcatcaccac gtcgcgcggt gcctcgaggt
     2461 cgcgggcgaa gtcaccgatg tggccgaggc ccgggtcgag actgcgcacc tggcggagca
     2521 cgagccgcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncgaag
     2581 tcgagcacga acgtctgcac ctgttgatca ggtgacaacg ccgcgttgat gcgggtccgc
     2641 cacgtgtcgg ccacggcgcc cacctggtcg atcagtgctc tgaccgccgt atcggtcaac
     2701 accaccgcac cgtcgggcac cagcgtcgag gacgcctggc gttcgatcga gaccacaccc
     2761 gatcggacgc ggacgttgat gatctcgggg agttcgatct gctcggggtc ggggttggtg
     2821 acatcgacgt caccgtcctg cacgttgacg acgacgtcgg cgtcgcccgg ctggccgccc
     2881 ggggtcgcgg tgtaggtgac gacgccgttg ttcagctcca actcgtcgtc gaagcgggca
     2941 tgcaccgtca ggcccatcgc tccgctgagg tggtcgatgc cagccaagcg gcgctcttcg
     3001 aaggcctcga agctccaata cgaaccccac gcgcgcagga tcgcgcgctc gatggtcttg
     3061 tcgtggtcgt ccttgtcggg cagtgccgtg ggatcgaggt agcccgtgta gctggtgtag
     3121 agaccggctc cgttgaagcc ctcgatgtct tccaccgagc tcgacgaacg gnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncccccgg
     3301 cttcgacgaa tgctcccact gccgagacgc cgccgcggcg cgcgacgaga tctgcggcgt
     3361 actcggcgtc ggccgcggtc gggaattgcc cggcgtagtc gtccggccct tccaacacca
     3421 gccagcgggc gcgcgggtcg gccaacacct cggggtcggc gatgacacgg gcgatcagcg
     3481 ctcgcatccc ggcgatgtgc tcgatgtacg gacgcaccgt gatcgccagc gggtcgggcg
     3541 gcggtgtgag gcctggcgtg gccaacaagg tgatgaagcc ggccgacttg ccgccgatga
     3601 tcggtcgcca gctctcgacc tcggcctcgc tgatcgcccc gtcggtaccg agctggcgga
     3661 ccagatcggt gaggttgacg acatgtccca tgcgggagat gtccacgggg gtgaccgcgc
     3721 tgggagcgcg ctgctgcgag gcgatccact cgtcgtactg cgctttgctg atcagcgcga
     3781 ccgtgactcc ggactcggtt gccaccacga tcgcgtgggc gcgcacgcgt gccgcctggc
     3841 gcaacccggg gtcgtcggtg atccccacta nnnnnnnnnn nngttgcggg cgagcaggtt
     3901 gacgtgcgca agcggtgtct gcgggtcgga cgtcaacagc gcggaagccg gtggtagcca
     3961 ggtcggcacc cgttcggtga cgatgatgtc gtgctcggtc gactcggcca tcgccgcgcc
     4021 gtcggggccg acgtacagca gccgtccggc cgtgacgccg gggctgtaga ccgactctgt
     4081 tccagatgga acgaggtcct tgaagtgcac gattcgatcg gaccaggcga gaccttcggc
     4141 gaccatctgt tgcgcgaccg cttcctgctg cggcgagcgg atcacccatt gcaggcggtc
     4201 gaccacctcg ccaggcagga tcggtgcgag ccgagcgaag aactggccnn nnnnnnnnnn
     4261 nnnnnnnnnn nnnngtcgga gtactcgagt tcgagcagcc acgcgtccgt accgttgccg
     4321 gttgtgttgg tcgcatcggc cgcgtcgaag tgcacgatcg agccgacacc gagggtccgc
     4381 gggttggtat tcagcgacag gtcgtagaag cggggcgagt agagccggtc gcccgcccac
     4441 gtcaggccaa ggggcaacga gcccttcggt tgggcccggg cccaggcgta gatctgttcg
     4501 acgttgcgga actgcagctg ggcgaccggc gtctggcccg acccagcgac gggctggccg
     4561 ttgaggaggc ggaagtagaa ccactcgtcg tgcaggccga agaagcggct gtccatgatg
     4621 tgcgcgcgcg gcagacccgg tgcgtccggc ggctgatcga atgcggggat cgtgaacttg
     4681 atcgccgact gaccaccgac tcccccgcgg gccaacgcca ggagctctgc tgtggtgtcg
     4741 acccgtggta cggagtggtc gtcgaatccc cgctcgtcga tgtaggtcgg caccgggacc
     4801 gtcaccacga tcgtcgacgg cggcagttcg gtcgtggttg gggtcgtcgt ggttgcagtg
     4861 tgtgacgtcg tcgacggttg cgtcgaggga gggcctgcaa tcgacgtcac cggcgacgac
     4921 ccgttcgtcg tcgccggctc gccggtggtc gagcacgcaa ccagcgagat cgcgctcgac
     4981 acggccgcga tccaacagag cccacgtcgc gacagcatgt gcattgagac gcacgggagc
     5041 ccgttccggt tcccccagtt cgcacatcgg tacactgcct gccggctcgt ccgccgctgg
     5101 cggagcagtt ggggcggtag ctcagtggtt agagcaaggg actcataatc cccgggtcgt
     5161 gggttcgatc cccacccgcc ccacctggta gacagcactt ttctcgcacg gctgttgact
     5221 tctcgtggga cgcgtgggac gttttcgaga gtgtcgatcg agcagttgcc ctccggcaag
     5281 taccgagcgg tggtccgaca cggcggcgcg aagcgggcca gcgaggccgt gccgaccaag
     5341 gccgaggcgc gcatgctgga ggccaagctg aagctcgaga tgggctcgca gccgtcgatt
     5401 cgtgagcaac actcggtcgg cgaggtcgtg tccggctacc tcgcggacgg cgccgtgcgc
     5461 ctgtcgccag cgacgctcga cttctaccgc aagggccacg atgcgatgcc ggacgcgttc
     5521 aagcagcgac cggtgacgac ggtgacgccg ctcgtgctcg acagcctgta cgccgagctg
     5581 cgggccggcg gtgcgagcga gcacaagtgc cagaaggtgc accgtctgct gtcggcctcg
     5641 ttcaaccggg ccgtgcggta cggctggctg gcggcgaacc cgtgcctgca ggcggccaag
     5701 ccgaaggtag cgacgcgcga gatcgagccg ccgacgccgg agcaggtgcg tgagctgatc
     5761 agcgccgctg cggaggtgaa tgaagacttg gtcgtgtgtc tccgtctggc tgcggcgacc
     5821 ggcgcccgtc gtggcgagct cgtggcgttg acgtggtcgg acttcgtggg ggagcggctg
     5881 acgatccgcc gctcggtcgt cgagtccgat ggccgactga tcgagcgtcg aaccaagacc
     5941 ggctcgaagg ggcacagaac gatcgccgtc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6061 nnnnnncggt gtacgtgttc tcgttcgacg ccggtgtgac cccgtggcgc cccgactacc
     6121 tgtcgatggc gttcggccgc ctgtcgaagc gcaaggtccg cctccacgac ctccgccact
     6181 accacgccac ccagctcctg tccgcgggcg tgcccgtcgc gacggtgagc aagcgcctcg
     6241 gccacaccag cacggcggtc accctgaaca cgtacagcca ctggttgcca gaacaggatc
     6301 gcgaagccgc cgacgtgatg nnnnnnnnnn nnnnnnnnnn nnnnnnnagc agcttctcgt
     6361 tcagcagcgg aacgatgtgg aggttgatct ccctcgcgac gccgcgcctc aatccgtcga
     6421 gcacgtcgac gacgccgagc ccccgagccc aattggccgt cagctcgtcg atcgtgacgg
     6481 tgagtgcgat gtgcggatcg aagcgggcgg gaggcaagcg atctccgaag tcgaaggttg
     6541 cgacgacgtc gcccgccgcc agagcgccgc tgttgaacga gtacgtcgga acaggatctc
     6601 cctcgttcga cccccagtag gccggcatgt cgtgatcgat catgtgagcg accggtaaga
     6661 gcagccggtg cttgtcgatg ttgttgagcc ggccaacgat tcccagagca tcggtggccg
     6721 gatcgtgtcc gtagttcgcc tccgagaacg gctgcattgc ctcgacagca gccatgatcc
     6781 tcgcatccgt gatgccgggc tcgatgacga gctgccgagg gtttcccttg cgattcgtgc
     6841 gcgcctcgtg gagtgggaac gccgttcgat cgttgggctg gccgccgtcg agcagcacga
     6901 gctgccaggc gacgtggtcg agcgcgctgc ggaggttgtg gacgatgtcg ccaacgatgg
     6961 cgccagccat cgggtcgact acgggaagtt cgacgactcg gtattcgatc ctcgaggggt
     7021 tgccgtctgc tggagccctc gagagcgaga agctggatgg gttcgacgtt ggtgccagca
     7081 ctgactgaag ctcctcgatg tgctgcttcg ctcgcccgag cttgacgcgg acgccgtcga
     7141 ggttcggaga cacccgagca tgctcgcacn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna gaacacccat
     7261 cgacccgatc cggggatgat tggtcgcacc ggcgaagtcc cgttgcgaac ggcgcgcttg
     7321 agcgactcgg gatggatgcc ccatcgctcg gcgagctcct cggtcgtgag cgtgagccga
     7381 cgagcccgag ctgtgaggtc gacgacgtct tcggatgact tcatgccgtc cacggtgaac
     7441 cacctacgtc acggatcact acccgacttg gtcgcccagt gcggaatatc ctgtcggcgt
     7501 ggcaacgacg tcactggccg agctgcgcga acgacgcgac gagatcatcg cggtggcacg
     7561 cgctcgaggc gcctcgcacg tacgggtctt cggttcggtc gcccgtggcg acgccacgga
     7621 agcgagcgat gtggacttcc tcgtcgatct ggaacctggt cgcagcttga tggaccttgg
     7681 cgggctgctc atggacctcc aggacctcct gcagcggaac gtcgacgtcg tcaccgagcg
     7741 ggggctccgt cctcgggtcg cccagcgggt tctggcggac gctgtcgagc tgtgagaggc
     7801 gatcgcgacc ggatcctcga catgctcgag atgtgcgagc tgctcatgga gcacgccgct
     7861 gacgaacgca agctggccga cgatccggtg acccaagccg ccgcccagcg ctggatcgag
     7921 gtcatcggcg aagcagcgtc acacgtctcc gatgagctga agctcgccca cccagacgtc
     7981 gcctggcgcg agatcagtgg catccgcgtc atcctcgccc acggctactt ccacatcgag
     8041 gacgacatca tcggcaacgt cgtcgccggc gacatcccca ccctgcgttc ccagcttcaa
     8101 gcgatcctcg cctcgcttcc cgaacccagc tgatttccgc acgttcgtct actacccctc
     8161 ggcttctgta catctgcacc aacggtgctc gaacgccgtg atcgggcgtt ctgggcgcct
     8221 cgaacgcctt cccgggcctg gcccgcgttc accgaacgga accggccatg gcgtcgagat
     8281 agcgcacagt cgcagctcgc tgcagacgcc gagccgccgg cgggcagagg cgagcgagtg
     8341 ggtgcctcgg tcgcgatacg gcgacgatcc ggaacgtgac ctcaccgtcg tcgtcccgct
     8401 cgatggtgaa cgactcctca ccctgctccg ggtggatcgg aagcgtgcca tacgcgaacc
     8461 cgaagcggtt cggctcgtcg acgaccgaga cgacccggca cgtcacgtcg atgaacccga
     8521 gtgggagcgg cgcgctcatc gccacgacct ggccgacggc cggtggacca tcggcgcaca
     8581 ccatcagacc agcgcctcga tgcacctccc actctcggag ggccctggcc gcgccgtcga
     8641 acacgtctcc gccggccccg agtgcacggt cccaacgatc gagccggtac ccgggcggcg
     8701 cgttgttgcg gccgccaacc cgacggggga gtaggtcgtt tccgcctgtc gtgacgccgc
     8761 gaccatcgag cgcagctcgt cgtcggttgg acgcgtgagg cggatcggca cttggcaagt
     8821 ctcgcggatc tccacgatca cgtcaggaac aggtcgatgg tctcgcttgc cgggcgaggc
     8881 aagcgtccgg tcgggtagtg atccgggtag cggtggctgg cggtcgggta gtgatccgtg
     8941 acgtcgtcgg ttcacgctgc actgggtgac cagccagcgc ccccgacccg gtcgaccccg
     9001 aaaatgggcc tcggatgcgg agcgcatgcg cgcataccgt gcgcgcatgc gagagcgttc
     9061 ggagcgtctt gtcgatccaa gtttcgcacc ggcggccgta gtcgagaatc gccgccttcg
     9121 cgatcgcgtc gcagagctcg aagacgaacg ggatcgtctc tgggctcagg tcgttcggct
     9181 ccaggagaac ctgcggagcg tcggacgccg agagcacagc cgaggagcga acgaatcgac
     9241 aaccgccggg aacacgccgt cgttgtcccg cgcccagcgc cggcggctcg aacgcgagcg
     9301 agcccgaagg gagcgccgcg gtgggtagca ccacgatcgt caacagttgt tgacgatcgt
     9361 ggagacgccc ccaccccgct gtgacgggag cgttctctgc gagaatatga gccgatcggt
     9421 cagcgcggtc ggaaagatca gaagtgccga tctcggacgg gag
//
LOCUS       DAORNF010000022         9167 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_5591_length_9167_cov_1.803665, whole genome shotgun sequence.
ACCESSION   DAORNF010000022 DAORNF010000000
VERSION     DAORNF010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 9167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9167
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5591_length_9167_cov_1.803665"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="PLV68_01730"
     CDS             complement(<1..234)
                     /locus_tag="PLV68_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99984.1"
                     /translation="MLEWGDQLAEGQQLPVTSLVRAAEHGVHLRPDQALLDVDDANLI
                     RLAVFVWPNGTRLDRRPDVAVAGDTWPASPVQRT"
     assembly_gap    289..371
                     /estimated_length=83
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    623..632
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<633..3637)
                     /gene="drmD"
                     /locus_tag="PLV68_01735"
     CDS             complement(<633..3637)
                     /gene="drmD"
                     /locus_tag="PLV68_01735"
                     /inference="COORDINATES: protein motif:HMM:NF038317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DISARM system SNF2-like helicase DrmD"
                     /protein_id="HRD99985.1"
                     /translation="MLITAVVVPELGQVVRCRDRVWAPSRWLSRTRGAPSSLPTDPIA
                     GTRPHHLVRMSSLEDDGFGDELTVIWEIEAGTEVIPHQELPRPVPGQLDPPERLDAFL
                     DAVRWGAVATADSRALQAPFRSGITIEDYQLDPVVRALAMPRANLLIADDVGLGKTIE
                     AGLVVQELLLRHRARTVLVVCPAGLCVKWQEEMLDRFGLEFRIVNTDAVRQLRRDRGV
                     GANVFTSFPRLIVSIDWLKDTRAQSLLDEVLTGVDHRRAPRKFDLLIVDEVHSIAPPG
                     RGRYAVDSLRTTAIKRLAPHCEHRLFLSATPHNGYKESFTALLELLDPQRFARGVDPN
                     PEQLARVLVRRLKRDLREELPPKPDGSPRFPERRILAMPVDYPADERDAHGLLDRYAE
                     LRRGRHTSGSKRSAADFVTLLLKKRLLSSPVAFANTLAQHRDTLAKQAKAQAAASDAQ
                     LEAAWERSSDESTDDEGGELVAEVLATASRGQAPPSVEEEQILKELSGWVEARRNRPD
                     ARADALIEWLTETCRTTGGGWNDERVIVFTEYRDTQKWLVDLLLTHGFGGEGGERVAT
                     LYGGMDEDDRERTKNEFQARPDRSPVRILVATDAASEGIDLQRHCWRMLHWEIPWNPS
                     RLEQRNGRVDRHGQPNPFVEIRHFVPEGWEDDQSAFATELSFLSRVAFKVEQIRDDLG
                     SVGAVLADQVGDAMLGRRTRLDDAAIDQARSKPGTKVLQVERRLRETLARCHEQLQAS
                     IEEFGLTTERIERVVATALDLARQPALQPTEAVGEFRLPPLGGSWQRTSEGMTDRLSG
                     DELPFTFDRDLAANRDDVVFCHLGHRLVTQSLRLLRAEVWATGGNGGLARVTARPVPP
                     LPEVLESGDVGVLCHARLVITGADGHRLHEELVVAGGRLRRGRWSRIDTLRDLDALLR
                     TAGDQSIDTSELAEVDRWWESVVEPGLNSALERRAETVQESKRRELAERARAEADAVR
                     AVLTDLQRQIAADLDTLGAERAEQLSLFTA"
     gene            4033..4578
                     /locus_tag="PLV68_01740"
     CDS             4033..4578
                     /locus_tag="PLV68_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99986.1"
                     /translation="MSATAATLAIASPRAGGVAVGAAGVAAAGWVASGMLTAIPAGIG
                     LAAVAATDLSTHHFSLRTLGWASSFVVAALIADSIVLVSWDRLGVALVGSAAVAIVLV
                     VGWLGTAGLAFGDVLLVPFAIALPLYLSALGAAVTVGVALVAAALSAVLQAVRVGVNR
                     SRAVPLAPPLLLGWITGVMVG"
     gene            complement(4556..4978)
                     /locus_tag="PLV68_01745"
     CDS             complement(4556..4978)
                     /locus_tag="PLV68_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99987.1"
                     /translation="MLPGANSARYVVTAGLAGSFATRGSVRIVVTAASYRSSSAWSTD
                     SKGTGVVVGTSDVGVVVGESDVAGTDGPVVVVDEAAVSGTDDAGGWTVAGELVEVVAT
                     SAAGTPEGSVAPFPEQATSASIAAALATRRWLIRPSLL"
     gene            complement(4985..>5183)
                     /locus_tag="PLV68_01750"
     CDS             complement(4985..>5183)
                     /locus_tag="PLV68_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99988.1"
                     /translation="PPLDSITFPAASTTSRLLAEHITNDASVSDTSVTSISDESIIEP
                     RGPGRYERPSAASCAHDAKTL"
     assembly_gap    5184..5307
                     /estimated_length=124
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5308..>5724
                     /locus_tag="PLV68_01755"
     CDS             <5308..>5724
                     /locus_tag="PLV68_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99989.1"
                     /translation="MMVAGASRASAATPAECVEVSPLGFCLEWGTPDTETPGTGGSSG
                     GGGGDGPPCYWVTLDYDLGTDDPTVFVDYGLATPPEGVDVVWQVLECADGTLIDDVRW
                     SIPPEPGDIAEGVRGRIAGTLPEPAVASSPAPGIAA"
     assembly_gap    5725..5769
                     /estimated_length=45
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5770..6138
                     /locus_tag="PLV68_01760"
     CDS             <5770..6138
                     /locus_tag="PLV68_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99990.1"
                     /translation="VTAQECAGFCVTVRAAPSLTFNPAETDASAVNCAGSGTAYMPDG
                     PPADEQAALEGACAHTYALRTGVPGRPLTWPGSVTVTWTITWSATTGASGTLSPVTRT
                     TDLPRAVEEVQTVVNGGVSP"
     gene            6135..6809
                     /locus_tag="PLV68_01765"
     CDS             6135..6809
                     /locus_tag="PLV68_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99991.1"
                     /translation="MTSTLRPRPSTNGSSAQTARAAAAGPPRTPVRRRWGRMGVGLAA
                     AVLGAWIFAAMYMSAGDHADVLVVANGVERFEVIERSDLQVARLSESSDVEVVAADRI
                     DEFVGRIAGVDLVGDSLLHEAQVMSSGTDLVTDGEAVVGVLVGPADAPQGSLRRGAAV
                     TVVVRPVAGTDVRPIEIAGWVWDASAEVSSTREVAVEVVVPAGDSAAVSAAAADRRVS
                     IVVLAG"
     gene            6814..7581
                     /locus_tag="PLV68_01770"
     CDS             6814..7581
                     /locus_tag="PLV68_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99992.1"
                     /translation="MLLGLVSAKGAPGVTAASVALTAASGGLMVELDPSGGSLDCWLR
                     ATGEPGLIRAASMLRRQADPATLASCATEVVPGVRAFRAPTSGVLAETTIVALRDRLL
                     PSLSEFDVTLIVDGGRWARSQPTARRLAGCDAVVLVCAPTIEGVEAARWQIEQLEALV
                     ERVLLVPVGDRPYGPAEVAAATDAEVCGALAWDRSGLARLLDRGAGNGWHRTSLARSA
                     RQLLEGLGVHRPTTPSSGASDTGVVAGSGFESEATHA"
     gene            7574..8887
                     /locus_tag="PLV68_01775"
     CDS             7574..8887
                     /locus_tag="PLV68_01775"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HRD99993.1"
                     /translation="MLDDVERTLRRTVADEIGSYLSNASADGRPLPDELDQRQMARAI
                     LRRELDNRSRAALRTGESPLTADQEDILLERVLATAFSPAPELDLLLERDDVTDVFVN
                     GCDDVRLVLIDGRTEFAEPLARTDNELIEKIQTLARRGGHMEREFTPARPILDLQLPD
                     GSRLAAAAWVTKRPYIAVRRHLLVDADQKELVARDMYDDGLASLFAALVRARRNILIA
                     GGQGVGKTTFLRALLHECQPDERIVVLEQEPELHLDASPGRHDHVLLFMERAANTEGA
                     GAVSLADLGRAIKRFGPRRIVVGEVRGPEVIDMFEAMTQGISGSMCTIHADSSWSVFP
                     RLPVYARAGGRDWATSDVLQLAALALDVIVFVARDRGGQRVVAEVRSVNRFDLDAGRV
                     VTDEWFQPGPSGRAVRNPSAPIPVGLLDELVDHGYDPTLHESGLL"
     gene            complement(8947..>9167)
                     /locus_tag="PLV68_01780"
     CDS             complement(8947..>9167)
                     /locus_tag="PLV68_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99994.1"
                     /translation="LGTPIRQLPCTLAPYAAGHPARGRRSQRGDRPARQDESYEERGD
                     RDKGAPPDHPLEPRETNHPMLWDRGQSR"
BASE COUNT         1319 a         2957 c         3093 g         1536 t
ORIGIN      
        1 ggtgcgctgc acagggctgg ccggccaggt gtcgccggcg actgcgacgt cggggcgccg
       61 atcgagtcgt gtgccgttcg gccatacgaa cacggcgagg cggatgaggt tggcgtcgtc
      121 gacgtcgagc aacgcctgat cggggcggag gtggacgccg tgctcggcgg cacggacgag
      181 cgacgtgacc gggagctgct gcccctcggc gagttggtcg ccccactcga gcaccgtcgc
      241 aaggagccat tggcgaagcg cggacctcgt gcctgggtca gtggtggcnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn naaggcgccg tctgggtcga cgaggttcag ccagtcgtcg atgctcgctg
      421 ccggcgtcgg gccgcgtcga gggttccgcg ccatcagttc gtctcctcag ggatcagcag
      481 ggtgactgct gccgggaaga tgtggcagtc cgggtcggtg aagcgtcgct gcaccgcttc
      541 gacttcgcgt tcgatttcct cggggatctc gtcgaggcgg cggcggagcg catcgatgtc
      601 gcgttgcgat tgctcgcgtt ctnnnnnnnn nngcggtaaa caggctgagc tgttcggcgc
      661 gttccgcgcc gagtgtgtcg aggtcggcgg cgatttgacg ttgcaggtcg gtgagcaccg
      721 cccgcacggc atctgcctcg gcccgagcgc gttcggcgag ctcacggcgc ttggactcct
      781 gcacggtctc ggcacgtcgt tcgagagcgc tgttcagccc tggttcgacg acgctctccc
      841 accaccggtc cacttcagcg agctcgctgg tgtcgatcga ctggtcgccg gcagtccgaa
      901 gcagggcgtc gagatcgcgc aacgtgtcga tgcgactcca tcggccgcgg cgaaggcgac
      961 cgcccgcgac cacgagttcc tcgtgcaggc ggtggccatc ggcgccagtg atcacgagtc
     1021 gggcgtgaca caggacgccg acatcaccgg actcgaggac ttcggggaga ggcggcaccg
     1081 gccgggcggt gacgcgtgcg agacctccgt taccgcccgt tgcccacact tcggcccgga
     1141 gcaggcgcag cgattgggtg acgagtcggt gcccgaggtg gcagaacacc acatcgtcac
     1201 ggttcgcagc gagatcgcga tcgaaggtga acggcagctc gtcgcccgag agccgatcgg
     1261 tcataccttc cgaggttcgt tgccacgacc cgcccagcgg cggtagccgg aactcgccaa
     1321 ccgcttctgt cggctgcaaa gcaggctggc gagcgaggtc gagcgccgtc gcgacgaccc
     1381 gctcgatccg ctcggtcgtc aagccgaact cttcgatgga tgcctggagc tgctcatggc
     1441 agcgggccag cgtctcgcgc agccgccgct ccacctggag caccttggtg ccgggcttcg
     1501 agcgcgcctg gtcgatcgct gcgtcgtcga gtcgggtgcg tcgaccgagc atcgcgtcgc
     1561 ccacctggtc ggccagcact gccccgaccg atccgaggtc gtcgcggatc tgctcgacct
     1621 tgaatgccac tctcgacaag aagctgagct cggttgcgaa cgctgactgg tcgtcctccc
     1681 agccctcggg gacgaagtgg cggatctcga cgaaggggtt cggttggccg tggcggtcga
     1741 cgcgtccgtt gcgttgctcg aggcgcgacg ggttccacgg gatctcccag tgcagcatcc
     1801 gccagcagtg gcgttgcagg tcgatgcctt cagacgccgc gtcggtggcg acgaggatcc
     1861 ggactggcga ccgatcgggg cgggcctgga actcgttctt cgtgcgctcg cgatcgtcct
     1921 cgtccatgcc cccgtacaag gtggcgaccc gctcgccccc ctcaccgccg aagccgtggg
     1981 tgagcaggag atcgacgagc cacttctggg tatcgcggta ctcggtgaag acgatcaccc
     2041 gctcgtcgtt ccacccgccg ccagtcgtgc ggcacgtctc ggtgagccac tcgatgaggg
     2101 cgtcggcgcg agcatcgggt cggttgcggc gggcctccac ccatccgctc agctccttga
     2161 ggatctgctc ctcctcgacg ctgggcggtg cttggcctcg tgacgcggtc gcgagcacct
     2221 cggcgaccag ctcaccgccc tcgtcgtcgg tcgactcatc gctgctgcgc tcccacgctg
     2281 cctcgagctg cgcgtccgat gccgcagcct gggctttggc ttgcttggcc aacgtgtcgc
     2341 ggtgctgcgc caaggtgttc gcgaacgcca ccggcgacga cagcaaccgc ttcttcaaca
     2401 gcaacgtgac gaagtcggca gccgaccgct tcgaaccgct cgtgtgacgg ccacgacgca
     2461 gctcggcata acggtcgagc agcccgtgtg cgtcccgctc atcggccggg tagtcgaccg
     2521 gcatcgccaa gatccgacgc tcagggaacc gtggcgaccc atcgggcttc ggcggcaact
     2581 cttcgcggag atcacgcttg agacgacgaa ccaacacccg ggcgagctgc tccggattcg
     2641 ggtcaacccc ccgtgcgaac cgctgcggat cgaggagctc caacagcgca gtgaagctct
     2701 ccttgtagcc gttgtgcgga gtcgccgaca ggaacaaccg gtgctcacaa tgcggagcga
     2761 ggcgcttgat cgccgtcgtc cgcagcgagt ccacggcgta gcggcctcga cccggcgggg
     2821 cgattgaatg cacctcgtcg acgatcagca agtcgaactt gcgaggcgcc cgtcggtggt
     2881 cgacaccggt cagcacctca tcgagtagcg actgcgctcg cgtgtctttc aaccagtcga
     2941 tcgacacgat cagccgaggg aacgacgtga acacgttcgc cccgacaccc cggtcgcgac
     3001 gaagctgccg aaccgcgtcg gtgttcacga tccggaactc gagcccgaac cgatcgagca
     3061 tctcctcctg ccacttcacg cagagaccgg ccggacacac cacgagcacc gtccgagcac
     3121 gatgacggag cagcagctct tggacgacaa ggccagcttc gatcgtcttg ccgagaccaa
     3181 catcgtccgc gatcagcagg tttgcccgcg gcatcgctag cgcacggact accgggtcca
     3241 gctgatagtc ctcgatcgtg atgccggaac ggaagggtgc ttgcaacgca cgagagtcgg
     3301 ccgtggccac cgcaccccaa cgaaccgcgt cgaggaaggc atcgagacgc tccggtggat
     3361 caagctgtcc gggcaccggt cggggaagct cctgatgcgg gatgacctcc gtcccagcct
     3421 cgatctccca gatcaccgtc agctcatcgc cgaacccgtc gtcctccagc gacgacatcc
     3481 gcaccagatg atgcggacgg gtgccggcga tcggatccgt cggcagcgac gaaggtgccc
     3541 ccctggttct cgacagccag cgtgacggtg cccaaacgcg gtcccggcac ctcacaacct
     3601 ggcctagctc aggaacgacc accgccgtga tcagcatgac gacgcagtcg ccctagccag
     3661 cgtctgaccg tttcgccggc tggaactcag catcgaggcc gcccatcttt cccgtcgccg
     3721 ttgcccatct gtggacatcc tagagaaggg gtgtcacgac catgagctgg ggcggctcgc
     3781 cgacggacca cctgtgcgcg ctgaatgcgt tcccaacatc actgcttgtg cacttgtact
     3841 cggctgaatc agaatgttcg gtaacacggg ctccctcgag aggagagtga gtagtcgtgt
     3901 ggccagacac ccagtaccga actgaatcgt cagcgggcgg aacgggtgcc cctcccttcg
     3961 cgtgcccgca cgcatttgac gggcggcccg atccagccga gcgacgagcc gtggtcggat
     4021 aggccagcgt ccatgagcgc gacggcggcc actctcgcaa tcgccagtcc tcgcgccggt
     4081 ggcgttgcag tgggggccgc gggtgtggcc gccgctggct gggttgcctc cgggatgctg
     4141 acggccattc ctgccgggat cggactcgcc gccgtcgcgg cgactgacct ctcgacacac
     4201 cacttctcgc tccgaaccct cggttgggcc tcctcgttcg ttgtcgccgc gctgatcgcc
     4261 gattccatcg tgctcgtctc ctgggaccgc cttggggtag cgctcgtcgg tagcgccgcg
     4321 gtcgcgatcg tgttggtggt cgggtggctt ggtacagccg gcctcgcatt cggcgacgtg
     4381 ctgcttgtgc cgtttgctat cgcgctaccc ctctacctat ccgcgctcgg cgcagccgtc
     4441 accgtcgggg tcgcgctcgt cgcggcggca ctgtccgccg ttctgcaggc tgtccgcgtc
     4501 ggcgtgaatc gatcacgggc ggtgccgctg gcgcctccac tacttcttgg atggatcaca
     4561 ggagtgatgg tcggatgagc cacctccgcg tcgccagtgc ggccgcgatg ctggcgctcg
     4621 ttgcctgctc cggaaacggc gccacggatc cctccggcgt gcctgccgct gatgtggcga
     4681 ccacttcgac gagttcaccg gcaaccgtcc aaccaccagc gtcgtccgtg ccagacacgg
     4741 cggcctcgtc gactacgacg accggtccgt cggtccctgc gacatcggat tctccgacca
     4801 caacgccgac gtcagatgtc cccacgacga cgcccgtgcc cttggagtcg gtgctccagg
     4861 cactgctcga ccggtacgac gctgccgtga ccacgatcct cacggatccg cgggtcgcga
     4921 acgatccggc gagtccggct gtcacgacgt atctggcact gttcgcgccg gggagcacct
     4981 tcgctcaaag cgtcttggcg tcgtgggcgc aggacgccgc ggaggggcgt tcataccgtc
     5041 cgggaccgcg tggttcgatg atcgactcgt cgctgatcga ggtgaccgag gtgtcggaca
     5101 ccgaggcgtc gttcgtgatg tgctcggcaa gcagccttga ggtcgtcgat gcggcgggga
     5161 acgtgatcga gtcgagcggc gggnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5281 nnnnnnnnnn nnnnnnnnnn nnnnnnngat gatggtggcg ggtgcctccc gagcgagcgc
     5341 tgcgacaccg gccgagtgcg tcgaggtctc gccgctcggc ttctgcctcg agtggggcac
     5401 cccggacacc gagacgccgg gcacgggcgg gtcgtccggc ggaggtggag gtgacgggcc
     5461 gccgtgctac tgggtcacgc tcgactacga cctcgggacg gatgatccga cggtcttcgt
     5521 cgactacggg ttggcgaccc cacctgaggg tgtcgatgtg gtctggcagg tcctcgagtg
     5581 cgccgacggg acgctgatcg atgacgtgcg ctggtccatt ccgccggagc cgggagacat
     5641 cgccgagggg gtccgcgggc ggatcgccgg caccttgccg gaaccagcgg tcgcatcgtc
     5701 gccagccccg ggaatcgccg cgatnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5761 nnnnnnnnng tcactgcaca ggagtgcgcc gggttctgcg tgaccgttcg agcggccccg
     5821 agcctgacct tcaacccggc cgagaccgac gcatcggcgg tcaattgcgc cggctctgga
     5881 accgcgtaca tgccggacgg gccaccggcc gacgaacagg ccgccttgga aggagcttgc
     5941 gctcacacct atgcgctgcg gaccggcgtg cctgggcggc cgctgacttg gccgggttcg
     6001 gtgacggtga cgtggacgat cacctggtcg gccacgaccg gtgcatccgg gacgctgtcg
     6061 ccggtgactc ggacaacaga tctgccgcgg gcggtggaag aggtccagac cgtcgtgaac
     6121 ggaggagtat cgccatgacc tcgacgttgc gaccacggcc gagcaccaac ggatcgagcg
     6181 cacagactgc ccgcgctgcg gccgctggtc caccacggac cccggtccgt cgtcgctggg
     6241 ggcggatggg tgtcggcctg gcggctgccg tcctgggcgc atggatcttc gcggcgatgt
     6301 acatgtcggc aggcgatcat gctgatgtcc tcgtcgtggc gaacggtgtc gagcgtttcg
     6361 aggtgatcga gcggtctgat ctgcaagtgg ctcgtctgtc tgagtcgtcc gatgtcgagg
     6421 tggttgcggc ggatcggatc gacgagttcg tcggacgtat cgcaggcgtc gacctcgtgg
     6481 gtgactccct gctgcacgaa gcgcaggtga tgtcgtccgg cacggatctc gtgaccgatg
     6541 gcgaggccgt tgtaggtgtt ctcgtcggtc cggccgatgc accccaaggg tcgctgcgtc
     6601 ggggtgcggc ggtgacggtg gttgtgcggc cggtcgcggg cacggacgtg cgcccgatag
     6661 agatcgcggg ctgggtgtgg gacgcctcgg ctgaggtgtc gagcacacgc gaggtcgcgg
     6721 tcgaggtcgt cgtaccagca ggcgactcgg cggccgtgtc cgcagcggcg gctgaccggc
     6781 gagtgtcgat cgtggtgttg gcggggtgac ccggtgttgc tcggactggt ctccgccaag
     6841 ggtgcgccgg gcgtcaccgc cgcgtcggtc gctctgaccg ccgcgtccgg tgggttgatg
     6901 gtcgaactcg atccgtcggg cggcagtctc gactgttggt tgcgcgcgac cggtgagccg
     6961 gggctgatcc gtgcggcatc gatgctgcgt cgccaggccg acccggcgac gctcgcgtcg
     7021 tgcgcgaccg aggtcgtgcc cggtgtccgg gcattccgtg caccgacttc gggggtgctc
     7081 gccgagacga cgatcgtcgc attgcgagat cgtctactcc cctcgctcag cgagttcgac
     7141 gtgaccttga tcgtcgacgg tggacggtgg gcccgatccc agccgactgc tcgccgattg
     7201 gccggctgcg atgcggtcgt gctggtgtgt gctccgacca tcgaaggggt cgaggctgcc
     7261 cgctggcaga tcgagcaact tgaagcgctg gttgagcgag tgttgctcgt cccggtcggc
     7321 gaccgcccgt acgggcctgc cgaagtggca gcggctactg atgccgaggt ctgtggcgcg
     7381 ctcgcctggg accggagtgg tcttgctcgg ctgctcgatc ggggcgccgg caacggatgg
     7441 caccgcacgt cgctggctcg ctccgctcgg caactgttgg aaggcctcgg cgtccatcgg
     7501 cccacaacgc cgtcctccgg ggcatccgac accggagtgg tcgccgggag cgggttcgag
     7561 tcggaggcga cacatgcttg acgatgtcga gcgcacgctt cgtcggaccg tcgcggacga
     7621 gatcgggagc tacctgtcca acgcgtccgc ggacggccgt ccgttgccgg atgagttgga
     7681 tcagcgtcag atggcacggg cgatcctgcg tcgtgagctc gacaaccgga gccgtgcggc
     7741 gttgcgaacc ggcgagtcgc cgcttaccgc cgaccaagag gacatcctgc tggagcgggt
     7801 gctcgccacc gccttctcac cggcacccga actcgacctt ctactcgaac gcgatgacgt
     7861 caccgatgtc ttcgtgaacg gctgcgatga cgtgcggttg gtgctgatcg acgggcgtac
     7921 cgagttcgca gaaccgctcg cccgcaccga caacgagctg atcgagaaga tccagacact
     7981 cgcccggcgg ggcggacaca tggaacgcga gttcacaccg gcgcgcccga tcctcgatct
     8041 gcaacttccg gatggttccc gactggccgc agccgcctgg gtgaccaagc gcccatacat
     8101 cgccgtgcga cgacacctcc tcgtcgacgc ggatcagaaa gaactcgtcg cccgcgacat
     8161 gtacgacgac ggcctggcgt cgctgttcgc cgcgctggtg cgagcccgcc gcaacatcct
     8221 gatcgccggc gggcagggcg tcggcaagac caccttcctg cgggcgctgc ttcacgaatg
     8281 ccaaccggac gaacgcattg tcgtgttgga gcaggagccc gagttgcacc tcgacgcgtc
     8341 accgggccga cacgaccacg tattgctgtt catggagcgg gcggccaaca ccgaaggtgc
     8401 cggcgccgtg tcgcttgccg atctgggccg ggcgatcaaa cgattcgggc cccgccggat
     8461 cgtggtcggc gaagttcgag gaccggaagt gatcgacatg ttcgaggcga tgacccaagg
     8521 catctccggg tcgatgtgca ccatccatgc cgactcgtcg tggtcggtgt tcccccggct
     8581 gccggtgtac gcccgggccg gcgggcggga ttgggcgacc agcgatgtgt tgcaactcgc
     8641 cgcgttggcg ttggacgtga tcgtgttcgt tgcccgcgac cgcggcggtc agcgcgtcgt
     8701 ggccgaggtt cgcagcgtca accggttcga cctcgacgcg ggacgcgtcg tcaccgatga
     8761 atggttccag cccgggccga gcggtcgtgc cgtgcggaac ccgtcggctc cgatccctgt
     8821 cggactgctg gacgagctcg tcgatcacgg ttacgacccg acactccacg aaagcgggct
     8881 gctatgacgg cgcttgccgc gttcgccggc ttcctcaccg tcgctggcgt ggccgtcgcc
     8941 gcgtcgtcag cgcgactggc cccggtccca gagcatcggg tgattcgtct cccgcggttc
     9001 gaggggatgg tctggcggcg cgcccttgtc gcggtcgccg cgttcctcgt agctctcgtc
     9061 ttgacgcgct ggccggtcgc cgcgctggct gcggcggccg cgggctgggt ggccggcggc
     9121 atacggggca agcgtgcacg gcagctggcg gatcggcgta ccgaggc
//
LOCUS       DAORNF010000023         9017 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_5712_length_9017_cov_1.762999, whole genome shotgun sequence.
ACCESSION   DAORNF010000023 DAORNF010000000
VERSION     DAORNF010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 9017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9017
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5712_length_9017_cov_1.762999"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    216..291
                     /estimated_length=76
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            391..768
                     /locus_tag="PLV68_01785"
     CDS             391..768
                     /locus_tag="PLV68_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99995.1"
                     /translation="MTGIVYDSAALIAAERDDRCLWLIHERALARGVEPVVPTAVLAE
                     SARPGMRNLRRLLAGCEVEPLGAESALVAGALRHATPGGTVIDAIVVESAVRRQAAVL
                     TADRGDIEALAGTVKKPIAIIDI"
     gene            complement(857..2251)
                     /locus_tag="PLV68_01790"
     CDS             complement(857..2251)
                     /locus_tag="PLV68_01790"
                     /inference="COORDINATES: protein motif:HMM:NF014741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HRD99996.1"
                     /translation="MDTDVNGAVDLGSGTEADREWDDHPLLWLQEEICLLAANLTAAE
                     ARWLAMVASFDRRAGWADDGAASCVAWLQQRVGLDRSTAYEKVRVATALQQFPAFASA
                     MAAGAVSYAKVRAITRIATDDNADELLEIALERSSHDVERYVAARRRCDESNETAAER
                     AHSERQLTYRCDGPTMIITVRIPVDMGATVIGAVDNHIDEHETAIPLRARRADAVVAL
                     AEHATAHHHGDECEGHRPLVTVHLTPNTINRDHDRDHDSASSGDEAAGSEVCCIAPGD
                     GLSAHPAAISAATAARVLCDAVIEGYTHPSDGDSPGGHLGRGRRTVSAKLKRALRLRD
                     QHCQAPGCDRTGWLDAHHIHHWLRGGSTMSSNLVCLCRFHHRMVHEGGWNITGNPNAI
                     GDLVFHRPDGTPVDGTQRLVYGHHHPISNHGCLPNALRPGPSDRLDEHLEAIDANHLI
                     QQRINQLIHTHQAA"
     gene            complement(2476..2595)
                     /locus_tag="PLV68_01795"
     CDS             complement(2476..2595)
                     /locus_tag="PLV68_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HRD99997.1"
                     /translation="MHDVQWLRYVLEYLAAGMTADEIVADFPELTADHIRVGA"
     gene            complement(2695..>3045)
                     /locus_tag="PLV68_01800"
     CDS             complement(2695..>3045)
                     /locus_tag="PLV68_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HRD99998.1"
                     /translation="YIFSSGSLSAPYAWFGNTPDGDLLPLFSAHFDTENAGPKRVADS
                     YRTIAAAIDTPPERIVFFSDLEAELDAAREAGWHTVGVRREGDQYFDQGVGDHTAIFS
                     FDEIDLSSGTPVAR"
     assembly_gap    3046..3157
                     /estimated_length=112
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3158..3419)
                     /locus_tag="PLV68_01805"
     CDS             complement(<3158..3419)
                     /locus_tag="PLV68_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD99999.1"
                     /translation="MTQVAPLAIVLDIEGTTSSTGFVHETLYPYSRERFAEWIAAHAE
                     RPDVAAQIESVRELAGEPDADVAKPNLDRVVWWLNHWLDGDQK"
     gene            complement(3440..>3757)
                     /locus_tag="PLV68_01810"
     CDS             complement(3440..>3757)
                     /locus_tag="PLV68_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRE00001.1"
                     /translation="EWPAAAATARDRFLAEHTHDDDEVRFFVEGSGAFYLRMGGEVAI
                     VVCAEGDLLSVPAGTTHWFDMGTSPRFAAIRFFRVPEGWVGHFTGDDIATRFETYDDL
                     VAA"
     assembly_gap    3758..3767
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3768..4022)
                     /locus_tag="PLV68_01815"
     CDS             complement(<3768..4022)
                     /locus_tag="PLV68_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin"
                     /protein_id="HRE00002.1"
                     /translation="MTTLVIHPEGDPAATTLHTTDPAEITAALAARGVRYERWTAGVA
                     LDPGAGQDEVLAAYASDVDRLRAEGYDTVDVARLVPNFSDP"
     gene            complement(4027..5607)
                     /locus_tag="PLV68_01820"
     CDS             complement(4027..5607)
                     /locus_tag="PLV68_01820"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HRE00003.1"
                     /translation="MHNTDRGHDLAGDLYCGVDVGTQGSKAGLYDVAGRCVGEGYAEH
                     HFDNLRPGWVEMDPEQIVAATVTAIATAVAHSGVDGHRVRAIALSGILCGPVFVDDDW
                     NPVRPLIPYLDVRASDEVAWLANEVEPRWVTESANAALDTYVMPAVYEWVRRHDPGAH
                     ARIRKVLSLAPFVGGRLAGLRAAQAYTDPSHLSGWIIGWNASTGRPSEQQMADLGIPV
                     EHAPQVHAPWDIVGELTRTMAERTGLAPGTPIAAGAGDVMQSNLGAGLTRPGMATDVA
                     GTASILTVGVTEPNPAITAVPGMLYSLSTLPGQALYWGYVKAGGMSLRWMRDEVLGRL
                     GDDSLYVQGDRAAAHVPPGSGGVLFAPYLSGGNPDNPDASGLWLGMSMDTDLGVLWRS
                     MLESIAFEYADFLDVFAANGAGVAEVIAVGGGARSPLWNQIKADVVGMPWRVPTRQDG
                     AVLANAALAALAVGAIDDLAGTVSAWVQAGEAHVPDAATHALYRRVRTARTDLLAGPL
                     RQVFSTLAPLRTLASPQE"
     gene            5716..>5959
                     /locus_tag="PLV68_01825"
     CDS             5716..>5959
                     /locus_tag="PLV68_01825"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRE00004.1"
                     /translation="MRADDVSADDVRAEPADAAPPRLAARGLTKSYGSVTVLDHVDLT
                     LAPGEVRALLGENGAGKSTLIKILSGVEQPTRGTIRY"
     assembly_gap    5960..5991
                     /estimated_length=32
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5992..>6329
                     /locus_tag="PLV68_01830"
     CDS             <5992..>6329
                     /locus_tag="PLV68_01830"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRE00005.1"
                     /translation="AIVAGISTLHQELSIVPGLSVAENVLLGNPLPTLAGTVRWREVA
                     RRASELFGQLGHRIDVRADVATLSPVDRTMTALARALSHEARVLILDEPTAALTDAET
                     RLLFGAIQRL"
     assembly_gap    6330..6455
                     /estimated_length=126
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6456..8196
                     /locus_tag="PLV68_01835"
     CDS             <6456..8196
                     /locus_tag="PLV68_01835"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRE00006.1"
                     /translation="QLVAAMAGRPIEAIFPPRISSPGEVVLRVRGLTGPRINGVDFDV
                     RRGEVVGIAGLAGSGRSEILRILAGAQRRRSGSVQLHGSEVPLRSVRSGHRAGVALVP
                     QERRSDGLVPASIERNTNVTTVARHAFGKVVMSRRRSRSHAQGHAEALDLRYRGLGQE
                     VLTLSGGNQQKVVLAKFLALDPTVLLLDEPTRGVDVGTKSQIYHLIEQRAADGQLRTA
                     RAARLGPPHPGDARGASRGRRRCSHHHRTRPAPRLLRKAHMTRLRPFAGPLVLLTMVL
                     FFAVNYGDRYFSKVNLVNNVLGSVAYVAIVAAGQTVVMVVGEFDLSVGGLAALSTVTT
                     AVLLATSSVLGEPQTPSGVFGAVLLGLVVGIGGGLVNGLLVSRLGILAFIATLATSLV
                     FQNFAAYRVDSKPVYNLVKHNFVDIARGQVLGIPNRVLIALGFGLVIWFLLDQTPLGR
                     KMYAVGGNPDAARYSGINVKNIKLIAFAICGLAAAAAGILQSANNATANLTAAGPWML
                     QSIAAVFLGMAMFRNGLANLPGTLLGVVMLRVLENGLNYTSINDSLQSVILGAAILVA
                     VLPLAVARLRSTR"
     gene            8193..>8621
                     /locus_tag="PLV68_01840"
     CDS             8193..>8621
                     /locus_tag="PLV68_01840"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HRE00007.1"
                     /translation="MTVSMLAQAVLAVDVGTTAVKAALVRDDVVIASAERPLTTTVGG
                     PGQAEQDPEQWWSAVCELTRELGGHLRADTDALALTGQMQDLVLLGAHGRPVRPALLY
                     SDMRAVAEHDALVARYGARWAEAVGGQPDATNVLAKWEWLR"
     assembly_gap    8622..8768
                     /estimated_length=147
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8769..>9017
                     /locus_tag="PLV68_01845"
     CDS             <8769..>9017
                     /locus_tag="PLV68_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00008.1"
                     /translation="WADGARDAGLPLPTLTAATAIVGHLDVVGAGETGLPVGLAVVHD
                     DGDAVATTVGLLGTALGRAYASLGTSAWVAVATAEPLAA"
BASE COUNT         1268 a         2768 c         3038 g         1440 t
ORIGIN      
        1 caatcagccg cggacgctca gaccacgcca ctcacacaaa attcccgaca gtcccaccat
       61 ccctcgtcaa cgggggggga ttgcatggtg ctaccagggt gctactgtgg cggtgtgagt
      121 tccgtcaagt atgcgaagcg gtccgtctcc ttgccccaag acttggatgc tgccttggag
      181 cgtcaggccg ccgaagaggg caccacggtg tcagcnnnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nccgagatcg
      301 cggaggtcga cgcgttgctc gatcaggccg gtgtgggctg aacgacggga gtgtcgaccg
      361 tggtccgtgc tgcgtgaatg cgctggtgga atgacaggca tcgtgtacga ctcggctgcg
      421 ctgatcgcgg ctgagcgcga tgatcggtgt ctgtggctga ttcacgagcg cgccttggct
      481 cgtggggtcg agccggtcgt gccgacagcg gtgttggctg aatcggcgcg gccagggatg
      541 cgcaatctgc gacgactgct cgcggggtgc gaggtggagc cgctcggtgc cgagtccgcg
      601 ctggtggccg gcgcgctccg acatgcgacg cccggtggca cagtcatcga cgcgatcgtg
      661 gtcgagagcg cggtgcgccg tcaggccgcg gtcctgaccg ccgatcgcgg tgacatcgag
      721 gcactcgccg gtacggtcaa gaagcccatc gccatcatcg acatctgatc cggcgcaaga
      781 ttgcgatcac gtggggatgt gacgccgtgg ctgtccccgg tggctactcg ctgtcgcccc
      841 cggacgactg tcggggttag gcggcttggt gggtgtggat gagttggttg atgcgttgtt
      901 ggatgaggtg gttggcgtcg atggcttcga ggtgttcgtc gagccggtcg ctggggccag
      961 gtcgcaacgc gttggggaga caaccatggt tgctgatggg gtggtggtgg ccgtacacga
     1021 gtcgttgggt gccgtcgacc ggggtgccgt cgggtcggtg gaagacgaga tcgccaatgg
     1081 cgttggggtt tccggtgatg ttccagccac cttcgtggac catgcggtgg tggaagcggc
     1141 agaggcagac caggttggat gacatggttg agccgccgcg gagccagtgg tggatgtggt
     1201 gcgcgtcgag ccaaccagtg cggtcacagc ccggtgcttg gcagtgttga tcgcgtagac
     1261 gtagtgctcg tttcagtttc gctgacacgg tgcggcgccc gcgcccgagg tgaccgcccg
     1321 gggagtcgcc gtcggatggg tgggtgtacc cctcgatcac cgcgtcgcac agcacgcgtg
     1381 ccgcggtggc cgctgagata gcggcggggt gtgcgctgag accgtcaccc ggagcgatac
     1441 agcacacctc gctacccgcg gcctcgtccc cgctgcttgc gctgtcatgg tcgcggtcat
     1501 ggtcgcggtt gatggtgttg ggggtgaggt gcacggtgac gagtgggcga tgcccctcac
     1561 actcatcacc gtggtggtga gcggtggcgt gttcggccag ggcgacgacc gcgtcggcac
     1621 gccgggcccg caacgggatt gcggtttcgt gttcgtcgat gtggttgtcg acagcgccga
     1681 tcaccgtggc acccatatcg acagggatgc gcacggtgat gatcatggtc ggcccgtcgc
     1741 agcggtaggt caactggcgt tcagagtggg cgcgttcggc tgcggtctcg ttcgactcgt
     1801 cgcaacggcg gcgcgcagcg acgtatcgct cgacgtcgtg agaggatcgt tcaagcgcga
     1861 tctccaacag ttcgtccgcg ttgtcgtccg tggcgatgcg ggtgatggca cgcaccttgg
     1921 cgtaggacac cgccccggcc gccatcgccg acgcgaacgc tgggaactgt tgcaacgcgg
     1981 tggcgacgcg gaccttctcg tatgcggtgg agcggtccag cccgacccgc tgctgcaacc
     2041 acgcaacgca agaggcggca ccatcgtcgg cccacccggc gcgccgatcg aacgacgcga
     2101 ccatcgccaa ccaccgcgcc tcggcagcag tcaagttggc tgcgagcagg cagatctctt
     2161 cctgcaacca cagcagtggg tgatcgtccc actcgcggtc ggcctccgtt cccgaaccaa
     2221 gatccacagc gccattcacg tcagtgtcca tacccccatc atgaacaatg ggtgtgacgc
     2281 ggattcccgc tgccgattgg gtacgcaacg ggatcagggg cgatcaacag attactcaat
     2341 aatcgcgttg ttggctactg atgtcgcccg ggggcgacag ttggtggtcg tcgtgtcggt
     2401 gtgcggtagg gccagcagcg actcttcggt cggtctcatg tagacgcgag ccggcgttcc
     2461 cgcagggcgg cgaactcaag cgccgacgcg aatgtggtcc gctgtgagct cggggaagtc
     2521 ggcgacgatc tcgtctgcgg tcatcccggc ggcgaggtat tcgagcacgt aacggagcca
     2581 ttgtacgtcg tgcatggttt gccgtttacg gcggttggct gttccgatac tgccaaggtc
     2641 gggatcttcg gcgcgtcgct gctcgccgcg ctcgctgggt cggtggtctt cgcgtcagcg
     2701 cgcgaccggg gtacctgagc tgaggtcgat ctcgtcgaag gagaagatcg cggtgtggtc
     2761 gccgaccccc tggtcgaagt actggtcgcc ttcgcggcgc acgccgacgg tgtgccatcc
     2821 ggcctcgcgg gcggcgtcga gctcggcctc cagatcggag aagaacacga tccgctcggg
     2881 tggtgtgtcg atcgcggccg cgatggtgcg gtacgagtcg gcgactcgct tcggccctgc
     2941 gttttcggtg tcgaagtggg cggagaacaa cggcaacagg tcgccgtcgg gcgtgttgcc
     3001 gaaccacgcg tatggggcgc tgagcgagcc ggacgagaag atgtannnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncct tctggtcgcc gtcgagccag
     3181 tggttcagcc accacaccac tctgtcgaga ttcggcttgg caacgtcggc gtcaggctca
     3241 ccggccaact cgcgcaccga ctcgatctga gcggcgacgt cgggccgctc ggcgtgggcc
     3301 gcgatccact ccgcgaagcg ctcgcggctg tacggataca gcgtctcatg gacgaagccg
     3361 gtcgacgagg tggtgccctc gatgtcgagc acgatcgcca gcggggcgac ctgagtcatg
     3421 tgagtcatgc cggatcggct cacgccgcga cgagatcgtc gtaggtctcg aaccgcgtgg
     3481 cgatgtcgtc gccggtgaag tggccgaccc atccttcggg aacccggaag aagcggatcg
     3541 cggcgaagcg cggcgacgtg cccatgtcga accagtgggt ggtgccggct ggcaccgaca
     3601 acaggtcgcc ctcggcgcag accacgatgg ccacctcgcc acccatgcgc aggtagaacg
     3661 cgccgctgcc ttcgacgaag aaccgcacct cgtcgtcgtc gtgggtgtgc tcggccagga
     3721 accggtcacg cgccgtggcg gctgcagccg gccattcnnn nnnnnnnggg atcgctgaag
     3781 ttgggcacga ggcgggcgac gtcgaccgtg tcgtatccct cggcgcgcag gcgatcgaca
     3841 tcggatgcgt aggcggcgag cacctcgtct tgcccggcgc cggggtcgag cgcgacaccc
     3901 gctgtccagc gttcgtagcg cacgccgcgg gcggcgagcg cggcagtgat ctctgccggg
     3961 tcggtggtgt gcagcgtggt ggcggcgggg tcgccttcgg ggtggatgac gagggtggtc
     4021 atggtgttac tcctgtgggg aagccaacgt gcgcagggga gccagggtac tgaacacctg
     4081 gcgcaatggg ccggccaaca ggtcggtacg tgccgtgcgg acgcggcgat agagggcgtg
     4141 cgtcgcggca tcgggtacgt gcgcctcgcc cgcctgcacc cacgccgaca cggtgcccgc
     4201 gaggtcgtcg atcgccccca ccgcgagcgc ggcgagggcg gcgttggcca acactgcgcc
     4261 atcctggcgg gtgggcacgc gccacggcat gcccacgacg tcggccttga tctggttcca
     4321 caacgggctg cgcgccccgc cgcccacagc gatgacctcg gccaccccgg caccattggc
     4381 agcgaacaca tcgaggaagt cggcgtactc gaacgcgatc gactccagca tcgatcgcca
     4441 cagcacgccg aggtcggtgt ccatgctcat gcccagccac aggccgctgg cgtccgggtt
     4501 gtcggggttg ccacccgaca ggtacggcgc gaacagcacg ccgccggagc caggcggcac
     4561 gtgggccgcg gcgcgatctc cttgcacata caacgaatcg tcgcccaggc ggccgagcac
     4621 ctcgtcgcgc atccaccgca gcgacatgcc gcccgctttg acgtagcccc aatacagcgc
     4681 ctggccgggc agggtcgaca gcgagtagag catgcccggc accgcggtga tcgccgggtt
     4741 gggctcggtg acgccgacgg tgaggatcga cgcggttccg gcgacgtcgg tggccatacc
     4801 gggccgggtc aggccagccc cgaggttcga ctgcatgacg tcgcccgcgc cagcggcgat
     4861 cggcgtgccc ggtgccagcc cggtgcgctc ggccatcgtg cgcgtcagct caccgacgat
     4921 gtcccacggt gcgtgcacct gcggcgcgtg ctcgacgggg atgcccagat cggccatctg
     4981 ctgttcggac ggacgaccgg tgctggcgtt ccagccgatg atccatccgc tgagatgcga
     5041 cgggtcggtg taggcctgcg cggcgcgcaa cccggcgagg cgaccaccca cgaagggggc
     5101 cagggacagc accttgcgga tccgggcgtg agcgccaggg tcgtggcggc ggacccattc
     5161 gtagaccgca ggcatcacgt aggtgtcgag cgcggcgttg gccgactcgg tgacccagcg
     5221 tggctcgacc tcgttggcca gccacgcgac ctcgtcgctg gcgcgcacgt cgaggtaggg
     5281 gatcagcggc cgcaccgggt tccagtcgtc gtcgacgaac accggcccgc acaagatgcc
     5341 ggacaacgcg attgcccgca cccggtggcc gtcgacgccg ctgtgcgcga ccgcggtagc
     5401 gatcgccgta acggttgcgg cgacgatctg ctcggggtcc atctcgaccc aacccggacg
     5461 aaggttgtcg aagtggtgtt cggcgtagcc ctcgcccaca cagcgtccgg cgacgtcgta
     5521 gagaccggcc ttggagccct gggtcccgac gtcgacgccg cagtacagat cacccgccag
     5581 atcgtggccc cgatcggtgt tgtgcatgcc gaagtgtcta gtcgctcgag gtgaccctcc
     5641 gtgtggccag ggtgccgtgt ccagggcgga actcgattgc gcggaggccc cgaactccga
     5701 caagactgct cgacaatgcg tgccgacgac gtgagtgccg acgacgtgag ggccgaaccg
     5761 gccgacgcgg caccaccgcg gctggccgcg cgcgggctca ccaagtcgta cgggtcggtc
     5821 acggtgctcg atcacgtcga cctcaccctg gcccccggcg aggtgcgggc attgctgggc
     5881 gagaacggcg cgggcaagtc gaccttgatc aagatcctct ccggggtcga gcagccgacc
     5941 agaggcacca ttcgctacan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nggcgatcgt
     6001 ggcgggtatc tccaccctgc accaggaact gtcgatcgtg ccggggctgt cggtggccga
     6061 gaacgtgctg ctgggcaacc cgttgccgac gctggcgggc acggtgcgct ggcgcgaggt
     6121 cgcccggcgg gcgagcgagc tgttcgggca gctcggccat cgcatcgacg tgcgcgccga
     6181 cgtcgccacg ttgagtcccg tcgatcgcac catgaccgcg ctggctcggg cgttgtcgca
     6241 cgaggcgcgg gtgctgatcc tcgacgagcc gactgcggcc ctgaccgacg ccgagacccg
     6301 cctgctgttc ggggcgatcc agcggctgcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntcagc tggtcgccgc catggctggg
     6481 cgtccgatcg aggcgatctt cccgccgcgc atctcctcgc ccggtgaagt cgtgttgcgc
     6541 gtccgtggcc tgaccggccc gcgcatcaat ggggtcgact tcgacgtgcg gcgcggcgag
     6601 gtggtcggta tcgccggcct tgccggatcg ggacgcagcg agatcctgcg catcctggcg
     6661 ggcgctcaac gacgccggtc ggggtcggtg caactgcacg gcagcgaggt gccgctgcgc
     6721 tcggtgcgct ccggtcatcg cgccggagtg gcgttggtgc cacaggagcg ccgttccgac
     6781 gggctcgtcc cggcgagcat cgagcgcaac accaacgtga ccacggttgc gcgtcacgca
     6841 ttcggcaagg tcgtcatgtc gcggcgacgc agccgcagcc acgcccaggg ccatgccgag
     6901 gcactcgatc tgcgctaccg cgggctcggc caagaggtgc tcaccctgtc gggcggcaac
     6961 cagcagaagg tggtgctggc caagttcctc gcactcgacc ccacggtgtt gctgctcgac
     7021 gaaccgaccc gcggcgtcga tgtcggcacc aagagccaga tctaccacct catcgagcaa
     7081 cgcgccgccg acggtcagct ccgaactgcc cgagctgctc ggcttggccc accgcatcct
     7141 ggtgatgcac gaggggcatc tcgtggccga cgtcgatgca gccaccacca ccgaacacga
     7201 cctgctcctc gcttgttacg gaaggctcac atgacccgcc tccgcccgtt cgcgggcccg
     7261 ctcgtcctgc tcaccatggt gctgttcttc gccgtcaact acggcgaccg ctacttcagc
     7321 aaggtcaacc tggtcaacaa cgtgctcggc tcggtggcct acgtggcgat cgtggccgct
     7381 ggccagacgg tggtgatggt ggtcggcgag ttcgacctat ccgtcggcgg cctggcagcg
     7441 ctctccacgg tgaccaccgc agtgctgttg gcgacgtcga gcgtgttggg cgagccgcag
     7501 accccgtcgg gtgtgttcgg ggcggtgttg ctcggtctcg tcgtcggcat cggcggtggg
     7561 ttggtcaacg gtctgctggt gtcacggttg ggcatcctgg cgttcatcgc cacgttggcg
     7621 acctcgctcg tcttccagaa cttcgccgcc taccgggtcg acagcaagcc ggtctacaac
     7681 ctggtcaagc acaacttcgt cgacatcgcc cgcggccagg tgctcggcat ccccaatcgg
     7741 gtcctgatcg cgcttggctt cgggctggtg atctggttcc tgctcgacca gaccccgctc
     7801 ggccgcaaga tgtatgccgt cggtggcaac cccgacgcgg cccgctacag cggtatcaac
     7861 gtcaagaaca tcaagctgat cgcgttcgcg atctgcggcc tggctgcggc tgccgcgggc
     7921 atcctccagt cggccaacaa cgcgaccgcg aacctcaccg ccgctggacc atggatgctc
     7981 cagtcgatcg ccgcggtgtt cctggggatg gcgatgttcc gcaacggcct ggccaacctg
     8041 cccggcacgt tgttgggcgt ggtgatgctg cgggtgctgg agaacggcct caactacacg
     8101 agcatcaacg acagcctgca gtcggtgatc ctgggtgcgg ccatcctggt cgccgtcctg
     8161 ccgttggcag tcgcccggct gcgctcgacc cgatgacggt gagcatgctg gcgcaggccg
     8221 tgctcgccgt cgacgttggc accaccgcgg tcaaggccgc gttggttcgc gacgacgtgg
     8281 tgatcgcctc cgccgaacgg ccgctgacca ccaccgtcgg cggccccggt caggcggagc
     8341 aagaccctga acagtggtgg tctgccgtgt gcgagctgac ccgggaactc ggcggtcacc
     8401 tgcgcgccga caccgacgcg ctggcactga ccgggcagat gcaggacctg gtgctgctcg
     8461 gtgcgcacgg ccgcccggtg cgccctgctc tgttgtattc ggacatgcga gccgtcgccg
     8521 agcacgatgc gctggtcgct cggtatgggg cgcgctgggc cgaagccgtt ggtggacagc
     8581 ctgacgcgac caacgtgttg gccaagtggg agtggctgcg tnnnnnnnnn nnnnnnnnnn
     8641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8761 nnnnnnnngt gggccgacgg cgcaagggat gcgggtctgc cgctgcccac cttgaccgcg
     8821 gccacggcga ttgtcgggca cctcgacgtg gtcggcgcgg gtgagaccgg attgccggtc
     8881 gggctggcgg tggtgcacga cgacggcgac gccgtcgcca ccacggtggg cctgctcggc
     8941 accgcgttgg gtcgcgccta cgcctcgctc ggcacgtcgg cctgggttgc ggtggccacg
     9001 gccgagccgc tggcggc
//
LOCUS       DAORNF010000024         8869 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_5849_length_8869_cov_1.821307, whole genome shotgun sequence.
ACCESSION   DAORNF010000024 DAORNF010000000
VERSION     DAORNF010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 8869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8869
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5849_length_8869_cov_1.821307"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="PLV68_01850"
     CDS             complement(<1..331)
                     /locus_tag="PLV68_01850"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRE00009.1"
                     /translation="MSETATRAGTVASTLAVSAEQATAYVRDGWWRTDTVGDIVTANA
                     AANPAGRAFTEGHTHLSWSELDRIAWVIAARLVERGIAPRSRVAVMMPDGPLVHAVYV
                     GVERAGAI"
     gene            complement(328..1470)
                     /locus_tag="PLV68_01855"
     CDS             complement(328..1470)
                     /locus_tag="PLV68_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012216258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRE00010.1"
                     /translation="MNAPGNTTPADMTPADTTSADTTLHGTTPGGEALVRLRNVRKEF
                     RVGAQRRNRSIATAVDDVSLEIHPGESLGLVGESGSGKSTVGRLLLRLLDPTSGTIEF
                     DGRDITRLSRKELRPLRSEMQAVFQDISGSLNAKMTIGQLVAEPLRFHRDVPRDQLAD
                     QSARMLEMVGLSRHHLARFPYELSGGQRQRVSLARALAVEPRLIVLDEPVSALDVSTQ
                     SQAVNLLGDLQARLGVAYLFIAHDLFVVHHVSHRIAVMYLGAIVELGTAEQVYSAPRH
                     PYTQALLSAIPHPDPNVELNRHRIILTGEVPSPTNIPSGCRFHTRCPYAMAVCATTPP
                     PEIVAADGGMVACHLHTEGPNLAGESVNSLPSPSSDAARATAEATA"
     gene            complement(1467..>2038)
                     /locus_tag="PLV68_01860"
     CDS             complement(1467..>2038)
                     /locus_tag="PLV68_01860"
                     /inference="COORDINATES: protein motif:HMM:TIGR01727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRE00011.1"
                     /translation="QARAKQYAFQFSGGMQQRVMMAIALVCRPKLLIADEPTTALDVT
                     VQAQITELIRELQTDLDMAVLFVTHDLGLLAGLAQKMIVMYGGQLIEEAPTAEVFLEP
                     RHPYTQALIFAAPHPDLKGQRLPTIPGAPPHPGAVPTGCRFHPRCAYFEARCTQERIE
                     ISRPAPERQVRCIRSAELHLSADLEAVRR"
     assembly_gap    2039..2060
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2061..2499)
                     /locus_tag="PLV68_01865"
     CDS             complement(<2061..2499)
                     /locus_tag="PLV68_01865"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRE00012.1"
                     /translation="MSEIVMSAAKEREPLLRIEHLSLELPNETGWVQILDDVSFDIAP
                     GELVGLAGESGSGKTMTALAIMGLLPKNPKLSGAITFAGRNLLELSKSQLRRVRGDDI
                     SMVFQEPITSLHPAWTVGEQIAEVVRAHEKVSRKASWDRAVEML"
     gene            complement(2496..3479)
                     /locus_tag="PLV68_01870"
     CDS             complement(2496..3479)
                     /locus_tag="PLV68_01870"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRE00013.1"
                     /translation="MTSTRPTSEQPPPSSAPAEITDDLPVIAAADTALTDAPPERLEG
                     RRLRAFLRNRPAMVSLVFLTIIVGTAALAPWIAPLSPTKNELRLNFATPWTRCVEGVC
                     SAPGLRILGTDDLGRDIFSRLLYASRISLLVGLGSVLVALVVALPLGMIAGYVGGKLD
                     WVMMRIVDIILSIPPLILVFAVAGVLGASIKNAILALGVFFVPLFVRLIRSEVLNLRS
                     SQLVETERAIGVPDSYILVRHVLPNIASPLIVQVSLSIGTAILAEASLSFLGLGVQAP
                     TPSWGIMLKSAFDFISRHPWMVLPPSLTIAFTVLALNIIGDGLRDALGKVR"
     gene            complement(3490..>3890)
                     /locus_tag="PLV68_01875"
     CDS             complement(3490..>3890)
                     /locus_tag="PLV68_01875"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRE00014.1"
                     /translation="TLAMSGVMIAELMRQIRSALVDTLETDYIRTARAKGLRERTVVN
                     KHALRVAASPALSVISVHVARSFGGAVVVEQVFSLPGLGKMTVNAITQRDLPILQGVI
                     PLAVLIAVVMTLLADVVNMTLNPRLKFQSR"
     assembly_gap    3891..4045
                     /estimated_length=155
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4046..>4368)
                     /locus_tag="PLV68_01880"
     CDS             complement(<4046..>4368)
                     /locus_tag="PLV68_01880"
                     /inference="COORDINATES: protein motif:HMM:NF040248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRE00015.1"
                     /translation="FLVFLLAIFLPGDEAVTLAGENASPQRVEEVRKALNLDQPLVTR
                     YVHWVGDAVQGDLGRSLFTKREVVSEIASRWVVTLQLVLGSLAVSLVIGIPAGIIAAR
                     NKGRW"
     assembly_gap    4369..4438
                     /estimated_length=70
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4439..5366)
                     /locus_tag="PLV68_01885"
     CDS             complement(<4439..5366)
                     /locus_tag="PLV68_01885"
                     /inference="COORDINATES: protein motif:HMM:NF015297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HRE00016.1"
                     /translation="MGRSDAKPLRSVLFVAGSDERELSEAPTYGADAIVIDIEEPRTP
                     FPESERVKTRALVREFLSSAPTGDGAPLWFVRVQPIDSGMTLRDLQAVMLPALAGILL
                     PKIQTASDVAAADALLTCLETETGRERGSTYIYPILETAQALRNAYEIAAASERVAYM
                     GGAVSRFGDIHQALGFRWTPEGRESLYLRSKVLVDVRAAGVQWPISGMWGGDLDDAAG
                     LRAWAEELRDIGYRGMMLGDPSLVAPVHEVFTPTEAEITYWQDLDRLAREAEEAGTGP
                     ILYGDPNQGEGHVVHIAHVGSARINLDWATRLG"
     gene            complement(5353..5814)
                     /locus_tag="PLV68_01890"
     CDS             complement(5353..5814)
                     /locus_tag="PLV68_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00017.1"
                     /translation="MPVEGTEVLFHHVADTPWLDIAANRDKARPGKGSEAKIKWLGGE
                     SATGPWVYYVEHPVAKPHKHLAPRIEYVLDGELEFFLGDDALAWYRGDTSVTGTLLGE
                     GSLSYVPSGTVYGYRITQATQLLHVFFENPIGRTIHVGKAAEEEAENHGTK"
     gene            complement(5977..6777)
                     /locus_tag="PLV68_01895"
     CDS             complement(5977..6777)
                     /locus_tag="PLV68_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00018.1"
                     /translation="MAGIDIVRADDAEVFDHHPIDGHRFAGAPGAPGAPDGIEARVVS
                     PAGYSLWLVAAALADRTSIELPPVHGDEVVAVLEGELDADGRICPAGGAVVIESGASP
                     TITARGRTRIVHMGPADPAVPQDGLNGPCTPGDAVHVVGPGGTFAAVDAHRDTHYFAD
                     STCPTCRLTLLYTSRSVEYISAAHSHSQAELIHLLHGELRLGAHTLRPGDTLAVDADR
                     RYGFRSGPDGFAFINYRRDASQQTVERGAPPLMEGGAVNGLVAVMDLR"
     gene            6960..>7757
                     /locus_tag="PLV68_01900"
     CDS             6960..>7757
                     /locus_tag="PLV68_01900"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRE00019.1"
                     /translation="MLISADSHVIEPGDLWVRELPASLRERAPRANRSPDNNHWYFTA
                     PGLNRGVDLTLSSSAGLTNAEVDAMLRDDPDAIIGSGGGSDPVARLYDLWLDDTVADV
                     LYPTAGLALLQMDDVELQEACFRVYNDWLADFCATDPVRLIGHALIPAWDADRAVAEM
                     QRARRLGLHGGLIWTSPPHDDSFFDRRYDRIWSAAVEMGMPMAIHTLAGQRESRALGT
                     FGTSVESSYYFTFRTRDEMQRTICELIVSGTFERHPDLEKFIGENLIN"
     assembly_gap    7758..7782
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7783..8135
                     /locus_tag="PLV68_01905"
     CDS             <7783..8135
                     /locus_tag="PLV68_01905"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00020.1"
                     /translation="GYRGFWGKFDHGLTMKPSEYFHRNVALTYINDVVGLNNVRFTGA
                     DNFMWSGDYPHGASTWPRSVESIGTETGTVGLDADTVRRLTVTNCAELYGIDLDVVSQ
                     PSPVIRERVAASGN"
     assembly_gap    8140..8232
                     /estimated_length=93
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8233..>8556)
                     /locus_tag="PLV68_01910"
     CDS             complement(<8233..>8556)
                     /locus_tag="PLV68_01910"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRE00021.1"
                     /translation="VLGVEATPADVLDADLAADDAVAAQIESIMATRPAEEWARRLAA
                     AGVPATVAETSGFDEWLEDRELLIPMDHPSFGAYWRLGPKLSLSGSNPVLAPPCAAGE
                     QSCDLL"
     assembly_gap    8557..8644
                     /estimated_length=88
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8645..>8869)
                     /locus_tag="PLV68_01915"
     CDS             complement(<8645..>8869)
                     /locus_tag="PLV68_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRE00022.1"
                     /translation="GRTNPPVNDSYADPGSALGAATAIMFGLWARTRTGAGQSMETTM
                     LASTGYIHSADMVDYAGAPGFVIADAGQHG"
BASE COUNT         1383 a         2868 c         2855 g         1310 t
ORIGIN      
        1 cgatcgcacc agcccgctcg acgccgacgt agacggcgtg cacgagcggg ccgtcgggca
       61 tcatcaccgc gacccggctg cgtggggcga taccccgctc gaccaaccgc gccgcgatca
      121 cccaggcgat gcggtcgagt tctgaccacg acaggtgcgt gtggccttcg gtgaaggcgc
      181 gtccggccgg attggcggcc gcgttggcgg tgacgatgtc gccgaccgtg tcggtcctcc
      241 accagccgtc gcgaacgtag gccgtggctt gctccgcact gaccgcgagc gtgctcgcga
      301 ccgtgcccgc gcgcgtggcc gtctcgctca cgcggtggcc tcggccgtgg cgcgggccgc
      361 atcgctggac ggcgagggca acgagttgac cgactcgccg gccaggtttg ggccttcggt
      421 gtggaggtgg cacgcgacca tgcctccgtc ggcagcgaca atctccggcg gtggcgtggt
      481 cgcacacacc gccatcgcgt acgggcagcg ggtgtggaat cggcagcccg aggggatgtt
      541 ggtcgggctc ggcacctcac cggtgaggat gatccggtgc cggttcagct cgacgttcgg
      601 gtccgggtgc gggatcgccg acagcagcgc ctgggtgtag gggtgccgcg gcgcgctgta
      661 cacctgctcg gccgtgccca gctcgacgat cgcaccgagg tacatcaccg cgatccggtg
      721 gctgacgtgg tgcaccacga acaggtcgtg ggcgatgaac aggtaggcga ccccgagacg
      781 agcctgcagg tcgccgagca ggttgaccgc ctgcgactgg gtcgacacgt cgagcgcgct
      841 caccggttca tcgagcacga tcagcctcgg ctcgaccgcc agcgcgcggg ccaggctgac
      901 ccgctggcgc tgaccgcccg acagctcgta cgggaagcgg gccaggtgat gacgggacag
      961 gcccaccatc tccagcatcc gcgccgactg atcggcgagc tgatcgcgcg gcacgtcccg
     1021 gtggaaccgc agcggctcgg cgaccagttg gccgatcgtc atcttggcgt tcagcgatcc
     1081 gctgatgtct tggaacaccg cctgcatctc gctgcgtagc ggccgcagct ccttgcggct
     1141 cagccgggtg atgtcacgcc cgtcgaactc gatggtgccg gaggtgggat cgagcagccg
     1201 cagcaacagc cggccgacgg tcgacttgcc cgaaccgctc tcgcccacca ggcccaaact
     1261 ctcgccgggg tggatctcca gcgacacatc gtcgaccgca gtcgcgatcg aacggttgcg
     1321 ccgttgggca ccgacgcgga actccttgcg cacgttgcgc aggcgcacca acgcctcacc
     1381 gccaggcgtc gtgccgtgga gtgtcgtgtc ggcagatgtt gtgtcggccg gtgtcatgtc
     1441 tgccggtgtc gtgttgccgg gcgcgttcat cgccgcaccg cctcgaggtc ggcgctgaga
     1501 tgcaactcgg cgctgcggat gcagcgcacc tgccgctcgg gcgcggggcg cgagatctcg
     1561 atccgctcct gggtgcagcg cgcttcgaag taggcgcagc gggggtggaa ccggcagccc
     1621 gtcggcaccg cgccgggatg cggcggggca cccgggatgg tcggcagtcg ctggcccttc
     1681 agatcgggat gcggtgcggc gaagatcaac gcctgggtgt acgggtggcg cggttcgagg
     1741 aacacctcgg ccgtgggtgc ctcttcgatc agctggccgc cgtacatcac gatcatcttc
     1801 tgcgccaggc cggcgagcaa gcccaggtcg tgggtgacga acagcaccgc catgtcgaga
     1861 tcggtctgca gctcgcggat cagctcggtg atctgggcct gcacggtgac gtcgagtgcg
     1921 gtcgtgggct cgtcggcgat cagtagcttc ggccggcaga ccagcgcgat cgccatcatg
     1981 acgcgctgtt gcataccgcc cgagaactgg aacgcgtact gcttggcccg cgcctgcgnn
     2041 nnnnnnnnnn nnnnnnnnnn gcaacatctc cacggcgcga tcccaactgg ccttgcggct
     2101 gaccttctcg tgggctcgca ccacctcggc gatctgctca cccaccgtcc acgccgggtg
     2161 caggctggtg atcggctcct ggaacaccat gctgatgtcg tcgccgcgca cgcgtcgcaa
     2221 ctgcgacttc gacaactcga gcaggttgcg cccagcgaag gtgatcgcac cgctcagctt
     2281 cgggttcttc ggcaacagcc ccatgatcgc cagcgcggtc atcgtcttgc ccgaaccgct
     2341 ctcgccggcc agaccgacca gctcacccgg agcgatgtcg aagctgacgt cgtcgaggat
     2401 ctgcacccac ccggtctcgt tgggaagctc gagcgacagg tgctcgatcc gcaacaacgg
     2461 ctcacgctct ttcgctgcgc tcatcacaat ctcgctcatc gcaccttccc cagggcgtcg
     2521 cggagaccgt caccgatgat gttgagggcc aagacggtga aggcgatcgt caagctgggc
     2581 gggagcacca tccacggatg gcggctgatg aagtcgaacg ccgacttgag catgatcccc
     2641 caactcggtg tgggtgcctg cacccccagg ccgaggaagc tgaggctggc ttcggccagg
     2701 atcgccgtac cgatgctgag cgacacctgc acgatcagcg gtgaggcgat gttgggcaag
     2761 acgtggcgca ccaggatgta gctgtcgggc acgccgatcg cccgctcggt ctcgaccagt
     2821 tggctgctgc gcaggttgag cacctctgac cggatcagac gcacgaagag cggcacgaag
     2881 aacacgccca aggcgaggat cgcgttcttg atgctcgcgc ccagcacgcc ggcgaccgcg
     2941 aacaccagga tcagtggcgg gatgctgagg atgatgtcga cgatgcgcat catcacccag
     3001 tcgagcttgc cgccgacgta tccggcgatc atgcccagcg gcagtgccac gaccagcgcg
     3061 acgagcaccg agccgagccc caccagcagc gagatgcgcg aggcgtagag caaccggctg
     3121 aagatgtcgc ggccgagatc gtcggtgccg aggatgcgta gaccgggcgc cgaacacaca
     3181 ccctcgacgc accgcgtcca cggggtggcg aagttgaggc gaagctcgtt cttggtgggg
     3241 ctgagtggag cgatccacgg cgccaaggcg gcggtgccga cgatgatcgt caggaacacc
     3301 aacgacacca tggccgggcg gttgcgcagg aacgcgcgca ggcggcggcc ttcgagccgc
     3361 tcgggcggag cgtcggtgag cgccgtatcg gcagcagcga tgactgggag atcgtcggtg
     3421 atctccgccg gggctgatga cggcggcggt tgctcggacg tcgggcgagt agaggtcacg
     3481 gtggggctcc tacctgctct ggaacttcag tcgagggttg agggtcatgt tgacgacgtc
     3541 cgccaacaag gtcatcacga cggcgatcag taccgcgagg gggatgaccc cttgcaggat
     3601 cggcaggtcg cgttgcgtga tcgcgttgac ggtcatcttg ccgagacccg gcagcgagaa
     3661 gacctgctcg accacgaccg cgccgccgaa ggagcgggcg acatggacgc tgatcaccga
     3721 cagcgccggc gacgcggcaa cgcgcagcgc atgcttgttg acgacggtgc gctcgcgcag
     3781 acccttggcc cgcgcggtgc ggatgtagtc ggtctcgagc gtgtcgacca acgccgaacg
     3841 gatctggcgc atcagctcgg cgatcatcac cccgctcatc gcgagcgtag nnnnnnnnnn
     3901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnntccac ctccccttgt tacgggccgc gatgatgccg
     4081 gccgggatgc cgatgaccaa ggagacggcg agcgagccga gtaccagttg cagcgtgacg
     4141 acccagcgcg acgcgatctc ggagacgacc tcgcgcttgg tgaacagcga ccggcccagg
     4201 tcgccctgga ccgcgtcgcc gacccaatgg acgtagcgag tgacgagggg ctgatcgagg
     4261 ttcagcgcct tgcgcacctc ctcgacccgc tgcgggctgg cgttctctcc agccagcgtg
     4321 accgcctcgt cgccgggcag gaagatcgcc aacaggaaca ccaggaacnn nnnnnnnnnn
     4381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngg
     4441 ccgagccgcg tcgcccaatc cagattgatg cgcgccgaac cgacgtgggc gatgtggacg
     4501 acgtgccctt caccctggtt cggatccccg tacaggatcg ggccggtgcc ggcctcttcg
     4561 gcctcgcggg cgaggcggtc gagatcctgc cagtaggtga tctcggcctc ggtcggcgtg
     4621 aacacctcgt gcactggcgc caccaggctc gggtcgccca gcatcatgcc gcggtagccg
     4681 atgtcgcgca gctcctcggc ccacgcccgc aggccggccg cgtcgtcgag gtcaccgccc
     4741 cacatgccac tgatcggcca ttggacaccg gccgcgcgga cgtcgaccaa caccttcgag
     4801 cgcaggtaca gcgactcacg gccctctggt gtccagcgga agccgagtgc ctggtggatg
     4861 tcgccgaaac gagacaccgc accacccatg taggccaccc gctcggaggc agcggcgatc
     4921 tcgtacgcgt tgcgcaacgc ctgcgcggtc tcgaggatgg ggtagatgta ggtcgacccc
     4981 cgctcgcggc ccgtctcggt ctcgaggcag gtcaacagcg cgtcggcagc agctacgtcg
     5041 gacgcggtct ggatcttggg cagcaagatc ccggccagcg ccggcagcat gaccgcctgc
     5101 agatcgcgca gcgtcatgcc gctgtcgatc ggctggacgc gcacgaacca caacggcgca
     5161 ccgtcaccgg tcggcgccga ggagaggaac tcgcgcacca gcgcccgcgt cttcacccgc
     5221 tcggactcgg ggaagggggt gcgcggctct tcgatgtcga tgacgatggc atcggcgccg
     5281 taggtgggcg cctcgctcag ctcgcgctcg tcgctcccgg cgacgaacag cactgaacgc
     5341 aacggcttgg catcacttcg tcccatggtt ctctgcttcc tcctcggcgg ccttgccgac
     5401 atgaatggtg cgcccgatcg ggttctcgaa gaacacgtgg agcaattgcg tcgcctgcgt
     5461 gatgcggtac ccgtagacgg tgccggacgg cacgtacgac agcgagccct caccgagcaa
     5521 ggtgcccgtg accgacgtgt cgccgcgata ccaggcgagc gcgtcgtcgc cgaggaagaa
     5581 ctcgagctcg ccgtcgagca cgtactcgat gcgcggggcc aggtgcttgt gcggcttggc
     5641 gacgggatgc tcgacgtagt acacccacgg tccggtggca gactcgccgc cgagccactt
     5701 gatcttggcc tcgctgccct tgccggggcg ggccttgtcg cggttggccg cgatgtccaa
     5761 ccacggcgtg tcggcgacgt ggtggaacag gacctccgtc ccttcgaccg gcatgtcgta
     5821 ccccctgtgc gtgttcctgt gggcggcggg caggcggaca caccgaacgg ctcagtcccc
     5881 acctgcccct aacatcttaa cgattaagac ttcagagatc atttcccagg agcacgggtg
     5941 ctgtcaagtc tccgcaccgg acacccctgg tcccgatcac cgcaggtcca tcaccgcgac
     6001 caggccgttg accgcgccac cctccatcag tgggggcgct ccccgctcga cggtctgttg
     6061 cgaggcgtca cggcggtagt tgatgaacgc gaacccgtcc ggtccgctac ggaagccgta
     6121 gcgacgatcg gcgtcgacgg ccagcgtgtc gccaggacgc agcgtgtgcg caccgagacg
     6181 aagctcgccg tgcagcaggt ggatcaactc ggcctggctg tggctgtgag ccgcggagat
     6241 gtactccacg gagcgtgagg tgtacagcag cgtcaggcgg caggtcgggc acgtggagtc
     6301 ggcgaagtag tgcgtgtccc tgtgggcgtc gacggcggcg aaggttccgc ccggccccac
     6361 gacgtgcacg gcatcgcccg gtgtacacgg cccgttgagg ccatcctgag gcaccgccgg
     6421 gtcggcggga cccatgtgca cgatgcgcgt ccgcccccgt gccgtgatcg tgggcgacgc
     6481 gcccgactcg atcaccacag caccgccggc cgggcagatc ctgccgtcgg cgtcgagctc
     6541 accctcgagc acggcgacca cctcgtcacc atgcacgggg ggcagctcga tcgaggtgcg
     6601 gtcggccagt gccgcggcga cgagccacag cgagtacccg gcagggctca ccacgcgggc
     6661 ttcgatgcca tccggggcgc caggcgcacc gggcgcaccg gcaaagcggt gtccgtcgat
     6721 cggatgatgg tcgaacacct ccgcatcgtc ggcacgcacg atgtcgattc cggccatgag
     6781 ccacgcccct ctccatcact caggctgcat ccattcaggg tgcatcgatc agcacgaacc
     6841 actcggtcgc tctgacttct taatcgttaa gatgatagca tctcttcagt gcacggtcga
     6901 ggggaccgtg accggtgcgg gcacggctcg ccagaacgtc ccagggggtt ctcaccgaca
     6961 tgctgatctc agccgactca cacgtgatcg agccaggcga cctgtgggtg cgcgagctcc
     7021 ccgcgtcgtt gcgcgaacgg gcaccacggg ccaaccgcag ccccgacaac aaccactggt
     7081 acttcaccgc tccgggtctg aaccgtggcg tcgacctgac gctgtcgtcg tcggccggtc
     7141 tgaccaacgc tgaggtcgac gcgatgctgc gcgacgaccc ggacgcgatc atcggctccg
     7201 gtggcggctc agatccggtg gcgcgcctgt acgacctgtg gctcgacgac accgtcgccg
     7261 acgtgctcta cccgaccgcc ggcttggcgt tgctgcagat ggacgacgtc gagctgcaag
     7321 aagcgtgctt ccgtgtgtac aacgactggc tggccgactt ctgcgccacc gatcccgtgc
     7381 gcctcatcgg ccacgcactg atcccggcgt gggatgccga ccgcgcggtc gcggagatgc
     7441 aacgagcccg caggctcggc ctgcacggtg gtctgatctg gacgtcgccc ccgcacgacg
     7501 acagcttctt cgaccggcgc tacgaccgca tctggtcggc cgcggtcgag atgggcatgc
     7561 cgatggcgat ccacaccctg gccggccagc gcgagagcag ggcgctcggc accttcggca
     7621 ccagcgtcga gtccagctac tacttcacct tccgcacccg cgacgagatg cagcgcacga
     7681 tctgcgaact gatcgtctcg ggtaccttcg agcgccaccc cgacctggag aaattcatcg
     7741 gcgagaacct catcaacnnn nnnnnnnnnn nnnnnnnnnn nnccggctac cgcggctttt
     7801 ggggcaagtt cgaccacggt ctgacgatga agccgtccga gtacttccat cgcaacgtcg
     7861 cgctgaccta catcaacgac gtggtcggcc tcaacaacgt ccgcttcacc ggggccgaca
     7921 acttcatgtg gtccggggac tacccgcacg gcgcgtcgac gtggccacgg tcggtcgaat
     7981 cgatcggcac cgagaccggc acggtcggac tcgatgccga cacggtgcgc cgcctgaccg
     8041 taacgaactg tgccgagttg tacggcatcg atctcgacgt ggtcagtcag ccgagcccgg
     8101 tgatccgcga gcgagtggcc gccagcggca actgacgccn nnnnnnnnnn nnnnnnnnnn
     8161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8221 nnnnnnnnnn nngagcagat cgcacgactg ctcgccagcc gcgcacggcg gggccagcac
     8281 cgggttggaa ccagacaacg acagcttcgg tcccaggcgc cagtaggcgc cgaacgacgg
     8341 atggtccatc gggatcagca gctcgcggtc ctcgagccac tcgtcgaagc ccgacgtctc
     8401 ggccaccgtg gccgggaccc ctgcggcagc gaggcgacgg gcccactcct cggcgggccg
     8461 tgtggccatg atcgattcga tctgggccgc gaccgcgtcg tcggccgcga gatcggcgtc
     8521 gaggacgtcg gcaggagtcg cctccacacc gagcacnnnn nnnnnnnnnn nnnnnnnnnn
     8581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8641 nnnnaagccg tgctggccgg cgtccgcgat cacgaagcct ggtgctcccg cgtagtcgac
     8701 catgtcggcc gagtggatgt agcccgtgct ggccaacatg gtggtctcca tcgactgccc
     8761 ggcgccggtg cgggtgcgcg cccacaggcc gaacatgatc gccgtcgccg ccccaagcgc
     8821 gctgccggga tcggcgtagg agtcgttgac cggcgggttc gtgcgaccc
//
LOCUS       DAORNF010000025         8832 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_5881_length_8832_cov_1.460522, whole genome shotgun sequence.
ACCESSION   DAORNF010000025 DAORNF010000000
VERSION     DAORNF010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 8832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8832
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5881_length_8832_cov_1.460522"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>212)
                     /locus_tag="PLV68_01920"
     CDS             complement(<1..>212)
                     /locus_tag="PLV68_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRE00023.1"
                     /translation="ARELALGFEFVEGNAELTPARLVGHFDPARVLTDGYDPEVFVDG
                     PLLTAMRDGSLLYVEEINRIPEETLNV"
     assembly_gap    213..318
                     /estimated_length=106
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(374..1537)
                     /locus_tag="PLV68_01925"
     CDS             complement(374..1537)
                     /locus_tag="PLV68_01925"
                     /EC_number="1.1.99.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012226367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NDMA-dependent alcohol dehydrogenase"
                     /protein_id="HRE00024.1"
                     /translation="MRSRAAVLWGTNQAWKLEEIEVDPPKVGEVLVQWKVAGMCHSDE
                     HFVTGDMVPPDDVLPLMGVDRFFPFIGGHEGAGVVVEVGEGVTSLAPGDHVSASFIPS
                     CGRCRPCSTGKQNLCDNGAKTLVGGMITDGTDRHHVVNADGSLAPVTLMAKLGTFSEY
                     ACVAEMSLIKVEPDLPLDAVALVSCGVATGFGSATNRAAVMAGDTVVVVGVGGIGINA
                     VQGAKLAGAARVIAIDPVEFKREKAMELGATHTFASMEEAMLPVMEMTWGTMADKVIL
                     TPGVMYGELLGQGQALCSKGGTIVVTAVAPMAQTEVSLNLFDLAMMNKEVKGTIFGSL
                     NPRADIPKLLGLYREGQLKLDELITRRYTLEQINDGYRAMRDGENIRGVIVYD"
     assembly_gap    1676..1685
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1686..2211)
                     /locus_tag="PLV68_01930"
     CDS             complement(<1686..2211)
                     /locus_tag="PLV68_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRE00025.1"
                     /translation="MRVVIAEDSVLLREGITRLLAEYDAEVVAALGDATRLLATVRET
                     SPDLVIADVRMPPTFTDEGLRAAVELRTVMTRIPVLVLSQYVEERFATELLGASTVGV
                     GYLLKERVADVSEFISACRRVAAGGTALDPEVVAQLLVRSRRREPLATLTPREHDVLS
                     LMAQGLSNTGIAQRL"
     gene            complement(2199..>2864)
                     /locus_tag="PLV68_01935"
     CDS             complement(2199..>2864)
                     /locus_tag="PLV68_01935"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRE00026.1"
                     /translation="GELRQRVSQLTVSRDASVEGASTELRRIERDLHDGAQQRLVGLA
                     MDLGLARDRIERGEDPERVAEMVTRAHDEAKLAIGELRQLVRGIHPQVLTDRGLDAAL
                     SALAARSPVPVDIDVRLATRPPGAVEAAAYFVVAESLANIAKHSRATRASVRVAEQQR
                     PADGRWVTVEIYDDGVGGASEHPGGGLAGLRDRVEAVEGRLRISSPDGGPTVLAVELP
                     CGS"
     assembly_gap    2865..2962
                     /estimated_length=98
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2963..>3250)
                     /locus_tag="PLV68_01940"
     CDS             complement(<2963..>3250)
                     /locus_tag="PLV68_01940"
                     /inference="COORDINATES: protein motif:HMM:NF025168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing protein"
                     /protein_id="HRE00027.1"
                     /translation="PPLQPTGGFFAKSFRYIGDGAGWRGLTFGLFDLGWGIVTFTTTI
                     VVWAVPLQLLTAALYQLFIPNRHRSWPYRINAGYELTGWGLAGVISGSVLIG"
     assembly_gap    3251..3364
                     /estimated_length=114
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3686..3749
                     /estimated_length=64
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3750..3930)
                     /locus_tag="PLV68_01945"
     CDS             complement(<3750..3930)
                     /locus_tag="PLV68_01945"
                     /inference="COORDINATES: protein motif:HMM:NF015302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HRE00028.1"
                     /translation="MLVLIGSLLTLLSAVGMVRFTDVFSRMHALAKASTAGVVVVLLG
                     AAVHLRHPNDITSLVL"
     assembly_gap    4097..4130
                     /estimated_length=34
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4192..4494)
                     /locus_tag="PLV68_01950"
     CDS             complement(4192..4494)
                     /locus_tag="PLV68_01950"
                     /inference="COORDINATES: protein motif:HMM:NF014012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HRE00029.1"
                     /translation="MVTRGSSIHTGIVACPLRTDSEAILTFLANVLSLTPGTMAVHVQ
                     NDPPLIYVHVLHLRSPDDTRRSFYNLEELTVRAFGSPEAILALGGPAADRMGVRQS"
     gene            complement(4759..>5358)
                     /locus_tag="PLV68_01955"
     CDS             complement(4759..>5358)
                     /locus_tag="PLV68_01955"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HRE00030.1"
                     /translation="HIGYMVMGLALFSVAGLAAAIFYAVHHILAMTTLFLVGGLIEHV
                     GGSSRMTELGNMVKSSPIVAMLFLIPALSLSGIPPLSGFVPKFALIGAGFSAGQYVVV
                     GVSLLVSLLTLFSVMKIWVGVFWSPAIQEPSGPVHRVGPLGGPAAMIVPTAVLFALGL
                     AVAIAAGPLYRLCERAATNLLTPQGYFDAVLGHAIGMGR"
     assembly_gap    5359..5476
                     /estimated_length=118
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5477..5857)
                     /locus_tag="PLV68_01960"
     CDS             complement(<5477..5857)
                     /locus_tag="PLV68_01960"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HRE00031.1"
                     /translation="MLTASYVLLTLGGRREQIRSGMTYVVISLLASTLFITALGLLYA
                     STGTLNMADLAQRIGDLPLGLRQTFAVLLVVVFGIKAGLFPLFSWLPDSYPTAPGPVT
                     AVFAGLLTKVGVYAMLRTQTLLFPG"
     assembly_gap    5874..5918
                     /estimated_length=45
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5919..6266)
                     /locus_tag="PLV68_01965"
     CDS             complement(<5919..6266)
                     /locus_tag="PLV68_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit D"
                     /protein_id="HRE00032.1"
                     /translation="MSNLVPLPIVLPMLGAALSVLVGRWKSVQRGLSVGILVATSVIS
                     VIMLIEVDRNGPLVMFAGEWRAPIGITLVVDRLSAIMLSVASLMLLAVLVYAIGQPGA
                     ERHHVGFQSVYMLL"
     gene            complement(6263..6823)
                     /locus_tag="PLV68_01970"
     CDS             complement(6263..6823)
                     /locus_tag="PLV68_01970"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit K"
                     /protein_id="HRE00033.1"
                     /translation="MTITLAFAAAGLFSLGTYLVLQRKLSRIIIGLALLSHGANALLM
                     TSGDAGTAPLIGSGDVTDFSDPLPQAMALTTIVITFGVTALLLAMAYRSWLLTHDDEV
                     EDDVTDRAVGRGGWSDKEVADELTAETTVMADEHTGTSDDVIGHHDGDDDDDDDDVAG
                     DDAGGDVAGGDAGQPIGTPAAGSGER"
     gene            complement(6820..7269)
                     /locus_tag="PLV68_01975"
     CDS             complement(6820..7269)
                     /locus_tag="PLV68_01975"
                     /inference="COORDINATES: protein motif:HMM:NF015970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MnhB domain-containing protein"
                     /protein_id="HRE00034.1"
                     /translation="MRRQVFLDQSARMVFHAVLIGSIYLLFAGHNQPGGGFAGGLVAG
                     AAIALRYLAGGIDDVRAISRFRPWTVLGSGLTLSAAIALAPLLKGQPVLTSMGHEFDL
                     PLLGHAYASTTLAFDLGVYTLVIGLVLMVFEAFGDDAADDTVDAEAR"
     gene            complement(7266..>7983)
                     /locus_tag="PLV68_01980"
     CDS             complement(7266..>7983)
                     /locus_tag="PLV68_01980"
                     /inference="COORDINATES: protein motif:HMM:NF024641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4040 domain-containing protein"
                     /protein_id="HRE00035.1"
                     /translation="TVYTALLRGTNTGATRLTGVIQNGSLPIYAGTVLLTAAIVPGAI
                     AIGRASWPGWPELAASWGQVAAIAAILGAALAACAVRRRFSSALFLGTVGYAMAGLFV
                     AQGAPDLALTQVAIETLSTVLFVLVLRRLPDRFERTSMPLRRIVRVIVAASVGIFVFF
                     LAITARGYRVDQPVSGEMIERSLPEAHGRNVVNVILVDIRGFDTMGEITVLASAAIGA
                     VALARVGRRRRDATRTERTT"
     assembly_gap    7984..8052
                     /estimated_length=69
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8053..>8298)
                     /locus_tag="PLV68_01985"
     CDS             complement(<8053..>8298)
                     /locus_tag="PLV68_01985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00036.1"
                     /translation="LPDAAPVRSPRRPDRRYLVSIVLLSAVSVALGVVPALGDTLLGA
                     AGHALDGRIDASHVHLAIWHGFTLELALTAAAIAAGLA"
     assembly_gap    8299..8459
                     /estimated_length=161
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8460..>8832)
                     /locus_tag="PLV68_01990"
     CDS             complement(<8460..>8832)
                     /locus_tag="PLV68_01990"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HRE00037.1"
                     /translation="FAPAFATVGIWRPLVLVLGATTMVLGGLRALRQHDLKLLLAYGT
                     VSQLGFLVVLFGIGTPEATLAATAMLLAHALFKAALFMAVGTVDRHTGTRDLRLIPGL
                     RRGWRSFRIMTFASAASMAGLP"
BASE COUNT         1409 a         2714 c         2750 g         1140 t
ORIGIN      
        1 acgttgagcg tctcttcggg gatgcggttg atctcctcga cgtacagcag cgagccgtcg
       61 cgcatcgccg tcagcagtgg cccgtcgacg aacacctcgg ggtcatagcc gtcggtcagc
      121 acacgggctg gatcgaagtg gccgaccaac cgggcgggcg tgagctcggc gttgccttcg
      181 acgaactcga acccgagcgc gagctcgcgg gcnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnncg ccggcgcccg accacgtcgg cacccaggcg ttgcagcaac
      361 gatgcgtcga tcatcagtcg tagacgatga cgccacggat gttctccccg tcgcgcatgg
      421 cccgatagcc atcgttgatc tgctcgagcg tgtaccgcct ggtgatcaac tcgtccagct
      481 tgagctggcc ctcgcggtag aggccgagca gcttggggat gtcggcgcgc gggttgagcg
      541 aaccgaagat cgtgcccttg acctccttgt tcatcatcgc caggtcgaac aggttgagcg
      601 acacctcggt ctgggccatc ggcgccaccg cggtgaccac gatcgtgccg cccttgctgc
      661 acaaggcttg accctggccg agcaactcgc cgtacatcac accgggggtg aggatgacct
      721 tgtcggccat ggtgccccac gtcatctcca tgacgggcag catcgcttcc tccatcgacg
      781 cgaaggtgtg ggtcgcaccc agctccatcg ccttttcgcg cttgaactcg accgggtcga
      841 tcgcgatcac ccgggccgcg ccggccaact tggcgccctg cacggcgttg atgccgatgc
      901 cgccgacgcc gaccacgacg acggtgtcgc ccgccatgac cgctgcccgg ttggtggccg
      961 aaccgaaccc tgtggcgacc ccgcagctga ccaaggccac cgcgtccaac ggaagatcgg
     1021 gctcgacctt gatcaacgac atctcggcga cgcacgcgta ctcgctgaag gtgcccagct
     1081 tggccatcag cgttaccggc gcgaggctgc cgtcggcgtt gacgacgtgg tgacgatcgg
     1141 tgccgtcggt gatcatgccc ccgacgaggg tcttggcgcc gttgtcgcac aggttctgct
     1201 tgcccgtgct gcacggccgg cagcgcccgc aactgggaat gaagctggcc gacacgtgat
     1261 cgcctggcgc caaggaggtg acaccttcac cgacttcgac gacgacacct gctccctcgt
     1321 ggccaccgat gaacgggaag aaccggtcga cgcccatcaa cggcaacacg tcgtcgggcg
     1381 gcaccatgtc gccggtgacg aagtgttcgt cgctgtggca catgccggcc accttccact
     1441 gcaccaagac ctcaccgacc ttgggcgggt cgacctcgat ctcttccagc ttccacgctt
     1501 ggttggtgcc ccacagaacc gctgctcgtg acctcatgag cgagcaatgt agcgaggtcg
     1561 aagtcagcgg gcgttgtcga ggtaggtcag cacggccaga acgcggcggt ggtcggtgtc
     1621 gctcacgggc aggtcgagct tggtgaggat cgaggtgatg tgcttctcga cagcgnnnnn
     1681 nnnnnccagc cgctgggcga tgccggtgtt gctcaggccc tgggccatca ggctcagcac
     1741 gtcgtgctcg cgcggggtga gggtggcgag cggttcgcgg cggcggctgc gcaccagcaa
     1801 ctgggcgacg acttcggggt cgagcgcggt gccaccggca gcgacgcggc ggcacgcgct
     1861 gatgaactcg ctgacgtcgg cgacgcgctc cttcagcagg taaccgaccc cgaccgtgct
     1921 ggctccgagc aactcggtgg cgaagcgctc ctcgacgtat tgcgacagca ccagcaccgg
     1981 gatgcgcgtc atcacggtgc ggagttcgac ggcggcgcgc agcccttcgt cggtgaacgt
     2041 cggtggcatc cgaacgtcgg cgatgaccag atcgggactg gtctcgcgca cggtcgcgag
     2101 caggcgggtg gcgtcgccca gcgcggcgac gacctcggcg tcgtactcgg ccagcaggcg
     2161 ggtgatgccc tcgcgcagca gcaccgagtc ttcggcgatc acgacccgca tggcagctcc
     2221 acggcgagca cggtcggacc accgtcaggg ctcgagatgc gcaaccggcc ctcgactgcc
     2281 tcgacccggt cgcgcaaccc ggccagccca ccgccgggat gttcgctcgc tccgcccaca
     2341 ccgtcgtcgt agatctcgac ggtgacccag cgcccatcag ccggccgctg ctgttcggcg
     2401 acccgcaccg acgcccgggt cgcccggctg tgcttggcga tgttggccag cgactcggcg
     2461 acgacgaagt aggccgcagc ctcgacggca cccgggggtc tcgtggcgag acggacgtcg
     2521 atgtcgacgg gcaccgggct gcgcgccgcc agcgcggaga gcgctgcgtc gagcccgcgg
     2581 tcggtgagca cctggggatg gatgccgcgc accagttggc gcagctcacc gatcgcgagc
     2641 ttggcttcgt cgtgggcgcg ggtgaccatc tcggccacgc gctcggggtc ctcaccgcgc
     2701 tcgatgcggt cgcgggccag accgagatcc atcgccaggc cgaccaggcg ctgctgggcc
     2761 ccgtcgtgca ggtcgcgctc gatgcggcgc agttcggtgc tggcaccttc gacgctggcg
     2821 tcacggctga cggtgagctg cgacacgcgc tgacgcaatt cgccnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nngccgatca gcaccgaacc ggaaatgacc ccggccagac
     3001 cccagccggt cagctcgtag ccggcgttga tgcgatacgg ccaactgcgg tggcggttgg
     3061 ggatgaacag ttgatacaga gcagcggtga gcagttgcag cggcaccgcc cacaccacga
     3121 tcgtggtcgt gaaggtcacg atgccccagc ccaggtcgaa cagcccgaag gtcagcccgc
     3181 gccaccccgc cccgtcgccg atgtaccgaa agctcttggc gaagaatcct cccgtcggct
     3241 gcagcggcgg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnncagggt gatgaacagc ccggcgctga gcgacagcat ggtgaccgcg acggtgaacc
     3421 aggcgatgcc gaggccgagg ttgatcagca ggtgcagcgt cgccttccac gtcgcggact
     3481 ggccgagcgg tgcgtaggcc cgatggacga tcgacattgc catgtcgtca gtgtggacca
     3541 ctcaggaagc agttccgatc aggcgtgctg gcatctccac gcgggggtta tccctactcg
     3601 ggcatctcgc gccggtctgc tgctgcggcc aggtcgttgc tcaactcgtc gacgtcgtgc
     3661 acggtgacgc cgatgccctc ggcccnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc gagcaccagc gaggtgatgt cgttggggtg
     3781 gcgcagatgc accgcagccc cgagcagcac gaccaccacg ccggccgtgc tggccttggc
     3841 cagtgcgtgc atgcgcgaga acacgtcggt gaaccgcacc atgcccaccg ccgacagcag
     3901 cgtcagcagc gagccgatca gcaccagcac ctcgccgatc atcgtcccct gctctcgatg
     3961 aagcgggcca ccatcgccgt gccgacgaaa ccgaccagag cgatcaccac cagcaccggc
     4021 aggaacgctc cccgcccggt ggccaccgcc tggacggcga tcgcgcagcc gccgctgacc
     4081 agcaatccgt tgagcgnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn acgagacggt
     4141 agaagaacag cacgccagag atcagcaacg ccgcgaacga gatcgtgatg atcatgactg
     4201 acgcaccccc atgcggtcgg cggcggggcc gccgagggcc aggatcgctt cgggcgaacc
     4261 gaaggcccgc accgtcaact cttcgagatt gtagaagctg cgccgggtgt cgtcaggtga
     4321 gcgcaggtgc aggacgtgga cgtagatcag cggcggatcg ttctgcacgt ggaccgccat
     4381 ggtgcccggc gtgagcgaca acacgttggc caggaacgtc aggatcgctt ccgagtccgt
     4441 gcgcaggggg caggcgacga tgccggtgtg gatgctggag ccgcgcgtga cgatctcgcg
     4501 ggcgatgacc gatggctccg gcagcgatga ccacgttgga caccaccagg tgccacgcga
     4561 tcgaaccgat caggtgcagc agggcgatcg gtcgggggcg gccgagtcgt tcgctgtcgg
     4621 tgccgccggg tgtggggaac acgagcagca ggaacatcgc gatcacgatg ccgctgacca
     4681 cgttggcgac gctgaccgag ccccacagca gcagccacac cacggtcagg aacacgacgc
     4741 gcatcacccg gcggctcatc atcgtcccat cccgatcgcg tggccgagca cggcgtcgaa
     4801 gtagccctgt ggtgtgagca ggttggtggc ggctcgctca cacaggcgat acaacggtcc
     4861 ggcggcgatc gctacggcca ggccgagcgc gaacagcacc gcggtcggca cgatcatcgc
     4921 ggccggacca cccagcggtc cgacgcggtg gaccggtccg gacggctctt ggatcgccgg
     4981 gctccagaac acgccgaccc agatcttcat caccgagaac agggtcagca ggctgacgag
     5041 caagctgacc ccgaccacca cgtactgccc tgcggagaac cccgcgccga tgagcgcgaa
     5101 cttggggacg aagcccgaca gcggggggat gcccgacagg ctgagcgccg ggatgaggaa
     5161 cagcatcgcg acgatcggcg acgacttgac catgttgccc aactcggtca tgcggctgct
     5221 gccaccgacg tgttcgatga ggccgcccac gaggaacagc gtcgtcatcg ccaggatgtg
     5281 gtggaccgcg tagaagatcg ccgcggccag cccggccacg ctgaacagcg cgaggcccat
     5341 caccatgtaa ccgatatgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5461 nnnnnnnnnn nnnnnngccg gggaacagca gcgtctgggt gcgcagcatc gcgtagacgc
     5521 cgaccttggt gagcaacccg gcgaatactg cggtgacagg accgggtgcg gtcgggtagc
     5581 tgtcgggcag ccatgagaac agcgggaaca gaccggcctt gatgccgaac acaaccacca
     5641 gcagcacggc gaaggtctgg cgcagaccca ggggcagatc gccgatgcgc tgcgccaggt
     5701 cggccatgtt gagcgtgccg gtcgaggcgt agagcagccc gagggcggtg atgaacagcg
     5761 tcgatgcgag caggctgatg accacgtagg tcatgcccga cctgatctgc tcgcggcgcc
     5821 cgcccaaggt gagcagcacg tagctcgcgg tcagcatgat ctcgaacgag acgnnnnnnn
     5881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnaa gcagcatgta gaccgactgg
     5941 aacccgacgt ggtgccgctc ggctcccggt tggccgatcg cgtagaccag taccgccagc
     6001 aacatgagcg acgcgaccga gagcatgatc gccgacaggc gatcgacgac cagcgtgatg
     6061 ccgatcggcg cccgccactc gccggcgaac atcaccagcg gaccgttgcg gtcgacttcg
     6121 atcaacatga tcaccgagat cacgctggta gcgacgagga tgccgacgct gaggccgcgc
     6181 tggaccgact tccagcgtcc gaccagcacc gacagggcgg cgcccagcat gggcagcacg
     6241 atgggcagag ggaccaggtt gctcatcgct cgccacttcc cgccgcaggc gtcccgatcg
     6301 gctggcctgc atcgccgccg gcaacatcgc cgccagcatc atcgccggca acatcgtcat
     6361 cgtcatcgtc gtcgtcgccg tcgtgatggc cgatgacgtc gtcgctcgtg ccggtgtgct
     6421 cgtcggccat cacggtggtc tccgcggtca actcgtcggc gacttccttg tcgctccacc
     6481 cgccgcggcc gacggcgcgg tcggtgacgt cgtcttcgac ctcgtcgtcg tgtgtcagca
     6541 gccagctgcg ataggccatc gccagcagca acgcggtgac gccgaaggtg atgacgatgg
     6601 tggtcagcgc cattgcctgg gggagcgggt cgctgaagtc ggtgacgtcg cccgagccga
     6661 tcagcggtgc cgtgcccgcg tcgccggacg tcatcaacaa tgcgttggcg ccgtggctca
     6721 acagcgcgag tccgatgatg atgcggctca gcttgcgctg cagcaccagg tacgtgccga
     6781 gcgagaacag tcccgcggcg gcgaacgcga gcgtgatcgt catcgcgcct ccgcgtcgac
     6841 cgtgtcgtcg gcggcgtcgt cgccgaaggc ttcgaacacc atcaacacca gcccgatcac
     6901 cagggtgtag acgcccaggt cgaacgccag cgtggtcgac gcgtaggcgt gacccaacag
     6961 tggaaggtcg aactcgtggc ccatcgaggt cagcaccggc tgacccttca acagcggggc
     7021 gagggcgatc gcagccgaca gcgtgagacc gctgcccaac acggtccatg ggcggaagcg
     7081 gctgatcgcg cgcacgtcgt cgatgcctcc ggccaggtag cgcagggcga tcgcggcccc
     7141 ggcgacgagc ccgccggcaa agcccccacc gggctggttg tgccccgcga acaacaggta
     7201 gatcgacccg atcagcacgg cgtggaacac catgcgcgcc gactggtcga ggaacacttg
     7261 gcgcctcatg tggtccgctc cgttcgtgtc gcatcacgac gacgccgtcc gacgcgggcc
     7321 agcgcgaccg cgccgatggc ggcagaggcg agcacggtga tctcgcccat ggtgtcgaag
     7381 ccgcggatgt cgaccaggat cacgttgacc acgttgcggc cgtgcgcctc gggcagcgag
     7441 cgctcgatca tctcaccgga gaccggttgg tcgacccggt acccgcgggc cgtgatggcc
     7501 aggaagaaca cgaagatgcc gacggacgcg gcgacgatca cgcgcacgat gcgccgcaac
     7561 ggcatcgagg tgcgttcgaa gcggtcgggt agacggcgca gcaccaacac gaacagcacg
     7621 gtcgaaaggg tctcgatcgc gacctgggtc agtgccaggt cgggtgcgcc ctgggccacg
     7681 aacaacccgg ccatcgcgta gcccaccgtg ccgaggaaca gggcagagct gaatcggcgg
     7741 cgcacggcgc aggctgcgag tgcggccccc aggatcgccg cgatcgccgc cacctgtccc
     7801 caacttgcgg ccagctcggg ccaccccggc cacgatgcgc ggccgatcgc gatagcaccc
     7861 ggcacgatcg ctgcggtgag cagcacggtg ccggcgtaga tcggcagcga gccgttctgg
     7921 atgaccccgg tcaagcgggt ggcgccggtg ttggtgccgc gcaacaacgc ggtgtacacc
     7981 gtgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8041 nnnnnnnnnn nncgccaacc ctgccgcgat cgcggcggcg gtgagagcca gttccagggt
     8101 gaacccgtgc cagatcgcga ggtggacgtg gctggcgtcg atgcgaccgt cgagggcgtg
     8161 tcccgccgcg cccaacagcg tgtcgcccag cgcgggcacc accccgaggg ccaccgacac
     8221 ggccgagagc agcacgatcg agacgaggta gcggcggtcg ggccggcggg gcgagcggac
     8281 cggcgcggca tcggggagnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng
     8461 gcaggccggc catcgatgcc gccgatgcga acgtcatgat gcggaagctg cgccaccccc
     8521 gccgcaagcc ggggatcagg cgcaggtcgc gggtgccggt gtggcggtcg accgtgccga
     8581 ccgccatgaa cagcgcagcc ttgaacagcg cgtgggccag cagcatcgcc gtcgcggcaa
     8641 gcgtggcttc gggtgtaccg atgccgaaca acacgaccag aaaccccagt tggctcacgg
     8701 tgccgtaggc cagcagcagc ttgaggtcgt gctggcgcaa cgcgcgcagt ccgccgagca
     8761 ccatcgtggt tgcgcccagc accagcacca gcggccgcca gatgccgacc gtggcaaagg
     8821 ccggggcgaa gc
//
LOCUS       DAORNF010000026         8673 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_6032_length_8673_cov_1.963449, whole genome shotgun sequence.
ACCESSION   DAORNF010000026 DAORNF010000000
VERSION     DAORNF010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 8673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8673
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6032_length_8673_cov_1.963449"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>8673)
                     /locus_tag="PLV68_01995"
     CDS             complement(<1..>8673)
                     /locus_tag="PLV68_01995"
                     /inference="COORDINATES: protein motif:HMM:NF015140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HRE00038.1"
                     /translation="SGTLTVTDSTYLDPRSSIDNSKRYDLHRTLRTEGYTGIHDGTFT
                     IHPGAEWRIGGNIGTDTEDSTAEPVVHNQGVMVVDVDDGFVTLSGVRLDNAGTLRLPQ
                     FNALTLGPGLHTNAGTIEIAFLAFLTVAGHLRLDETSVLAMSDPSYLGEIIVPGQITL
                     GGHLHVAMQPLGSDADPFAFIQASPATGTVTGTFATVDATGWDVVYGDHDVTVVYADT
                     PPPVPPTIVIAPQQVLESAGTAAVIVQGVGGNVALTVPGGTVESSASAPADFLPFVGS
                     VVVPPNGSVVVPITIVNDALDENDETFRLVVGGVSGTISILDDDPEPIVRILDTEVIE
                     GNFGFRELVYPIELVDLFGAPAPSGRDVWVFAATSDGGSAVDGEDYDARGSVVMFPPG
                     THTATFSVSIYPNDFADGDRTVEVTLSEPFNAQLDVNMLRALGTIVDDDEAEPPPEGE
                     VAAESNAGRAAVAAAVPEWGRAFDLDGFDLPSLGVAPFELPVVTDELGSAFGTQAALG
                     AVATPYGPMSGELAEVVAALRAAGITIDWVAGGVGGTPLPPSPTDLLQARTEITLADL
                     ARAGGFGGDDLNDDAALLTGLADAVDLDADFDLLADLTVTLVFGVDSDGFYLSAESAL
                     LIDVVADGTIAGTADAGGNAGATVAGSGHAEVAVGLIASGGPERLRLDDLVDLAADPF
                     DVLRPSVTGSASTELDLTAAPLALTWSSATTVTTDADGNVATPTTAHLTGTVDLPGFR
                     LGTSPAPTPAVITFDGTFAGGVWTLAGAATIDSDLRVDGFVVHALDGTATISPDAVAA
                     SGRLVLALGEGLTLVEVVLAIASTGWSGSATVTTDVLDVGPVHIDQPSLTVTATHDST
                     DDRGGPDGTTTVAVSVTAAGGRVDGPGGPAGEPLVRLSGVSGGFDSTGRVCVETEQID
                     GTIGGALSFTADDVEVCSPGPDGVTLRIGSVTAVAAELGELSVTITDLVLDADGRFGA
                     ESVAVEQPDGIAQRVGLAGIVPVDLTSLVLTFSDRDDEGRIDDLSHFVVALEGTVDPT
                     GFSGLPFDPVIQIGGDVITPASPSAARRVAFSAEIVSVDPLVVRPLDLGPLRLGFTGL
                     EVGDYTFTGHIAADGYADGVLLPGVCLEVGALGGFDTLDLDGELEACGTITETPTGPM
                     IVLEATATASLAVRGGIEIVDAALGARITAEMADGSVRIGAVLTGASVGRVTIPFGGV
                     ATVQVTDVALDFTPGPGEPALVIGGSLGQPGSGADVTFGGGAGVFDGWGGRIGGIGLT
                     GDLRPLALAGFFAEVTVPGTEQFGLPEFLPFEIDRIGLSLPGFPTGDVTDGVVLTPAL
                     LEGLRLRVSGGLVGTPAFPVSATVDGLEVDLGRMLAFNPLAPLDLATFPITNLDGVSF
                     AIEPSIDLGGLAVGGALTFGVVDVGGTPVLYGRISGDVSSPAFQAGADVVVSQYGPVL
                     LRITAPVGVPLGPTGLVLRSVTGAATFGPGGTVTPPRVGHPEDLLNELFDLPTDVDMT
                     PETIRTAVAAAVTSGSSIWNDGFAVALEGDLTHVAAVGMLSGRVTLAASFGAPAPGQP
                     QRVQLVGRGDISAFGIPLTDGLDIGGTLAVAGVLLDFVDPLAPSIDLAFQSPVPGSPL
                     AVVFPAQALVAAQIRTDGVITGIGAGLTTFVDGAVTGSIGVGGAMFADAFDRLAAELQ
                     ANRHSALAQLVIAANDPRAIDADLLHTRLVALLSSPDTAARVAGPLLQAIGAAIAASI
                     NDGSFGLTVAQAADALYTVIGGAAADALRVAGALFDPSLILRGTLQPLLLGIPLGSPT
                     AQVEVALDRNSFRFELDASMIESLKFAAHQVPVLGPTAEQLITLAALGGRDQLTFGVL
                     VPVPDFVELLLGGDFDTLDPSDPGQGWSITIGGAFQLHGLEAQVTGFMTAPDNAAFVD
                     TKIEKRWLADGTTPPDPDRIQITRPQDYDNLIRYGGIVLDGRLEVPRLLTDPVAVLSD
                     LPPMPDDALGIADWLADAAATLGQIETPLRMTAFAPGLDAVLANPNSETVQEWADAFV
                     FTRVFEGTSQTPESDPVARLLSLPIGEGRMLATSAGLEVTANVPLLGVDGSFILRVDD
                     RDGVPVPAGGLEVSMSAAAARAALADLGFPALFDIDATATVRAYTPGFDPASDDVLRR
                     RGGLAIGATFDAPGFVEDAAFMIVVDPIGTGAGPDFTATASVDRIGPFAGVEIDDVTL
                     TVAKLGTAVSIRAQGSANFGGTQVTVDGFLNPDLTGQLVFTATGSGSPTLGGVRFVEG
                     GFALTLHRDGGALVGEIGIGGTVELPSFLAARAGSATAAAAGCLNTDGDIELRLAIYG
                     LALGPNTTLGGTGAPLPIDPAAPCTLPPGVGELPNTAARIVVRTADGISRVVIDGAVR
                     IEGSGLALLTVTGAVSTAGVGSLAVSFGSGLSLSGFLITGGASLAFTPTSFTLSVDGR
                     VTVPNLVEDARVTGVITDAGIQSLAVSVAGLALPPLQVTSASLTLNRTAPSTYVLDVS
                     LAVSVPGLRRTGTPDASVTATGRIATTGDFDLAISGSAFTLNGTGLSSASVNLARTGD
                     LLQVAVAANFALWGSTLAVSGAAMVDPNGLSGSVALRTPGGPISVAGWTIGGDLQLAF
                     TVSGTGFSARISLLNGTVTVPGVGTLVANANMATDGTGSITISTPTGIRLGGASSPFV
                     GFGSFSLRQISGVVTFGASNVGVAVHPVGGTEAFRVNAPNFSISSNGAFSIATAGATL
                     GSASGLRLVIPSASLTAGPGGSNIALTVGAGSLTVPGLLDGIGGRPPALSTPSFAINS
                     GPFRYVLFDARTIDLGLLRLNGRIVFERTAAGVFQLGIEANSSGPANLDLGQLGRIDL
                     GNFFIASDGSFAVTASTRRIGIANPLFEVRDASFVFRQSGGVVELAVNSGRLHLPSLA
                     QPIT"
BASE COUNT         1494 a         2932 c         3080 g         1167 t
ORIGIN      
        1 aggtgatcgg ctgcgccagc gacggaaggt gcagcctgcc gctgttgacc gccaactcga
       61 ccacgccgcc cgactgacgg aacacgaacg acgcgtcgcg tacttcgaac agcgggttgg
      121 cgataccgat ccgccgcgtc gaggcggtga cggcgaacga accgtcgctg gcgatgaaga
      181 agttgccaag gtcgatgcga cccaactggc cgaggtcgag gttggcaggg ccggacgagt
      241 tggcctcgat gccgagctgg aacacacccg ccgcggtgcg ctcgaacacg atccggccgt
      301 tcaaccgcaa caagccgagg tcgatcgtgc gggcgtcgaa cagcacgtag cggaacggcc
      361 cggagttgat cgcgaacgac ggcgtggaca acgccggggg tcgcccgccg atgccgtcga
      421 gcagccccgg cacggtgagc gagccggcac cgacggtcaa cgcgatgttg gaaccgcccg
      481 gaccggccgt cagcgaggcc gacgggatca ccaagcgcag accggacgcc gacccgagag
      541 tggcacccgc ggtcgcgatc gaaaaggcac cgttgctgga gatggagaag ttgggggcgt
      601 tgacacggaa ggcctcggtg ccgcccaccg gatggaccgc gacgcccacg ttcgacgccc
      661 cgaaggtgac cacgccgctg atctgacgga gcgagaacga accgaagccg acgaacggcg
      721 acgacgcccc gccgaggcgg atgccagtcg gcgtgctgat cgtgatcgag cccgtgccgt
      781 cggtggccat gttggcgttg gcgacgaggg tgccgacgcc gggcacggtg accgtgccgt
      841 tgagcaacga gatgcgcgcc gagaagccgg tgccggagac ggtgaacgcg agctgcaggt
      901 cgccgccgat cgtccatccg gcaacgctga tcggcccgcc tggggtgcgc agcgcgaccg
      961 atcccgacag accgttcggg tcgaccatcg ccgcgccact gaccgcgagc gtcgagcccc
     1021 acagcgcgaa gttggcggcc acggcgacct gcagcaggtc gccggtgcgg gcgaggttga
     1081 cgctggcgga ggagagcccg gtgccgttca gggtgaacgc cgagccgctg atggccaggt
     1141 cgaagtcacc ggtggtggcg atgcgccccg tcgcagtgac ggaagcatcc ggcgtgccgg
     1201 tgcggcgcaa gcccggcacg gagacagcga gcgacacgtc gagcacgtag gtcgacggtg
     1261 cggtgcgatt cagtgtcagg ctggcactgg tcacctgcaa gggcggcaag gccaagccgg
     1321 ccacggagac ggcgagggac tggatgccag cgtcggtgat cacgccggtg acgcgggcat
     1381 cctcgaccag gttcggcacg gtgacgcgtc cgtcgacgga cagggtgaac gaggtcgggg
     1441 tgaaggccag cgacgcgccg cctgtgatca ggaagcccga caggctgagc cccgacccga
     1501 agctgaccgc caggctgccg acgccagcag tcgacacggc accggtgacc gtcaacagcg
     1561 cgaggccgct gccctcgatg cgtaccgctc cgtcgatgac cacccggctg atgccgtcag
     1621 cggtgcgcac gacgatccgg gccgccgtgt tcggcaactc gcccacgccg ggcggcagcg
     1681 tgcatggtgc cgccggatcg atcggcagcg gcgcgcccgt tccacccaag gtggtgttgg
     1741 ggccgagcgc caacccgtag atggcgaggc ggagctcgat gtcgccatcg gtgttgagac
     1801 aacccgccgc ggcggccgtg gcgctgcccg ctcgtgcggc aaggaagctc ggcagctcga
     1861 cggttccacc gatgccgatc tcacccacca gagcgccacc gtcgcggtgc aacgtcagcg
     1921 cgaacccacc ctcgacaaaa cgcacccctc cgagcgtggg cgagcccgag ccggtcgcgg
     1981 tgaacacgag ttggccggtg aggtccgggt tgaggaagcc gtcgacggtc acctgcgtgc
     2041 cgccgaagtt ggccgaaccc tgcgcccgga tcgaaacggc tgtaccgagc ttggcaacgg
     2101 tgagggtgac gtcgtcgatc tcgactccgg cgaagggtcc gatgcgatcg accgacgcag
     2161 tggccgtgaa gtcaggaccg gccccggtgc cgatcgggtc gaccacgatc atgaacgcgg
     2221 cgtcctcgac aaagcccggc gcgtcgaacg tcgccccgat cgcgagcccg ccgcggcggc
     2281 gcagcacgtc gtcgctggct gggtcgaacc ccggggtata ggcgcgcacg gtggccgtgg
     2341 cgtcgatgtc gaacagggcc gggaacccca ggtcagccag cgccgctcgg gccgcggcgg
     2401 cagacatcga tacctccaag ccgccagcag gtacgggtac gccatcgcga tcgtcgacgc
     2461 gcaggatgaa cgaaccgtcg accccgagca gcggcacgtt ggcggtgacc tccagcccgg
     2521 cgctggtggc gagcatgcgc ccttctccga tcggcagcga cagcagccgg gcgaccggat
     2581 cggactctgg ggtctggctg gttccctcga acacgcgggt gaagacgaac gcgtcggccc
     2641 actcctgcac ggtctcgctg ttcgggttgg cgagcaccgc gtcgagaccg ggagcgaacg
     2701 ccgtcatccg cagcggcgtc tcgatctggc ccagtgtggc ggccgcgtcg gccagccagt
     2761 ccgcgatccc cagcgcgtcg tcgggcatcg gcggcaaatc cgacagcacg gcgaccggat
     2821 cggtcagcaa gcggggaacc tccaaccgtc cgtcgagcac gatgccgccg tagcggatga
     2881 ggttgtcgta gtcctgtggc cgggtgatct ggatgcgatc ggggtcggga ggcgtggtgc
     2941 cgtcggcgag ccaccgcttc tcgatcttgg tgtcgacgaa cgcggcgttg tcgggcgcgg
     3001 tcatgaaccc ggtgacctgc gcctccaacc cgtgcagctg gaacgcccca ccgatcgtga
     3061 tcgaccaacc ctgaccggga tcggaggggt cgagcgtgtc gaagtcaccg ccgagcaaca
     3121 gttcgacaaa gtcgggcacc ggcaccagca cgccgaaggt gagttggtcg cgcccgccga
     3181 gggcggcaag ggtgatcagc tgttcggcgg tcggccccag caccggtacc tgatgggcgg
     3241 cgaacttgag gctctcgatc atgctcgcgt cgagttcgaa gcggaagctg ttgcggtcga
     3301 gcgcgacctc gacctgcgcc gtgggcgagc cgagcgggat gccgagcaac agcggttgga
     3361 gcgtgccgcg caagatgagc gatgggtcga acaacgcgcc cgcgacgcgc aacgcgtcgg
     3421 cggcagcgcc accgatcacg gtgtacagcg cgtcggcggc ttgagcgacc gtgaggccaa
     3481 acgatccgtc gttgatggag gcagcgatcg cggcgccgat tgcttgcagc aacggcccgg
     3541 cgacgcgggc ggccgtgtcg ggcgacgaca gcagcgcgac caggcgagtg tgcaacaggt
     3601 cggcatcgat cgctcgcggg tcgttggccg cgatcaccag ttgcgccagt gcgctgtggc
     3661 ggttggcctg tagctctgcg gccagacggt cgaaggcatc cgcgaacatc gctccgccga
     3721 cgccaatgct tccggtgacc gcaccgtcga cgaacgtggt caagccggcg ccgatgccgg
     3781 taatcacacc gtcggtgcgg atctgcgcgg cgaccagcgc ctgggccggg aacaccacgg
     3841 cgagcggact gccgggcacg gggctctgga aggcgaggtc gatcgagggg gcgagcgggt
     3901 cgacgaagtc gagcaggacg cccgcgaccg cgagcgtgcc gccgatgtcg aggccatcgg
     3961 tgagcggtat gccgaaggcc gagatgtcgc cgcgcccgac cagttgcact cgctggggct
     4021 ggccgggtgc cggcgccccg aacgacgcgg cgagggtgac ccggcccgac aacatcccca
     4081 ccgcggcgac gtgggtgaga tcgccttcga gtgcgacggc aaagccatcg ttccatatgg
     4141 aactaccgct cgtgacggct gccgcgacgg cggtgcggat cgtctctggg gtcatgtcca
     4201 cgtcggtcgg caggtcgaac agttcgttca acaagtcctc gggatgacct acccggggcg
     4261 gcgtcaccgt gccaccaggg ccgaacgtgg ccgcgccggt caccgagcgc agcaccagtc
     4321 ccgtcggccc cagcggcacg ccgacggggg cggtgatgcg caacaacacc ggcccgtact
     4381 ggctgacgac gacgtcagca ccggcctgga atgccgggga cgacacgtcg ccactgatcc
     4441 ggccgtagag cacgggtgtg ccaccgacgt cgacgactcc gaacgtgagc gcaccaccga
     4501 cggccaaacc gccgaggtcg atgctgggct cgatcgcaaa gctcacgccg tcgaggttgg
     4561 tgatcgggaa cgtcgccaga tcgagtgggg cgagcgggtt gaatgcgagc atccgaccga
     4621 ggtcgacctc caatccgtcg acggtggcag acacgggaaa ggccggtgtg ccgacgaggc
     4681 cacccgacac gcgcagccgc agtccctcca acagcgcggg cgtgagcacg acaccatcgg
     4741 tcacgtcgcc agtcgggaaa ccaggcagtg acaagccgat gcggtcgatc tcgaacggga
     4801 ggaactccgg cagcccgaac tgctcggttc caggcaccgt gacctctgca aagaacccgg
     4861 ccagcgcgag cgggcgcagg tcgcctgtca ggccgatgcc gccgatccgg ccgccccagc
     4921 cgtcgaacac gcctgcgccg ccaccgaacg tcacgtccgc acctgagccg ggctggccga
     4981 gcgacccacc gatcacgagt gccggttcac cgggtccggg cgtgaagtcg agtgcgacgt
     5041 cggtgacctg cacggtggcg acaccgccga acgggatcgt gacgcgcccg accgaggcac
     5101 cggtgagcac cgcaccgatg cgcacactgc catcggccat ctcggcggtg atgcgcgcgc
     5161 cgagcgcggc gtcgacgatc tcgatgccgc cgcgcaccgc gagcgacgcg gtggcggtcg
     5221 cttccagcac gatcatcgga ccggtgggcg tttcggtgat ggtgccgcac gcttccagtt
     5281 cgccatcgag gtcgagcgtg tcgaacccgc cgagcgcgcc gacctccagg cacacgccgg
     5341 ggagcaacac cccatcggcg taaccatcgg cggcgatgtg accggtgaag gtgtagtcac
     5401 cgacctccaa cccggtgaag cccaaccgca gcgggcccag atcgagcggt cggacgacga
     5461 gcggatcgac gctgacgatc tccgccgaga acgcgacgcg gcgcgcggcc gacggtgacg
     5521 cgggtgtgat cacatcgccg ccgatctgga tcacaggatc gaacggcagc ccggagaacc
     5581 cggtcgggtc gaccgtgcct tccagtgcga cgacgaagtg agacagatcg tcgatgcgac
     5641 cctcgtcgtc acgatcggag aaggtcagca ccaggctggt gaggtcgacg ggcacgatgc
     5701 ccgccaggcc gacgcgctgg gcgatgccat cgggttgctc caccgcgacc gactcggcgc
     5761 cgaagcggcc gtcggcatcg aggacgagat cggtgatcgt caccgacagc tcgccgagtt
     5821 cggccgcaac cgcggtgacc gagccgatcc gcagggtcac gccgtccggt cctgggctgc
     5881 acacctcgac atcgtccgcg gtgaagctga gcgcgccgcc gatcgtgccg tcgatctgct
     5941 cagtctcgac gcatacccgt ccggtgctgt cgaagccgcc gctgacaccg ctgaggcgca
     6001 cgagcggctc gcccgctggc ccaccggggc cgtcgactcg tccgcccgcg gcggtgaccg
     6061 acacggcgac ggtcgtggtg ccgtccgggc cgcctctatc gtcggtgctg tcgtgggtcg
     6121 cggtaaccgt cagcgacggt tggtcgatgt gcacggggcc gacgtcgagc acgtcggtcg
     6181 tcacggtcgc cgaccccgac caaccggtag acgcgatggc gagcaccacc tccaccagcg
     6241 tgaggccctc gcccagggcc agcacgagcc gccccgacgc ggcgaccgca tccgggctga
     6301 tggtggcggt gccgtcgagc gcgtgcacca cgaagccgtc gacgcgcagg tcgctgtcga
     6361 tggtggctgc accggccagc gtccacacac caccggcaaa ggtgccgtcg aacgtgatca
     6421 cggccggagt gggtgctggc gacgtgccca ggcggaaacc gggtaggtcg accgtgccgg
     6481 tgagatgggc ggtggtcggt gtcgcgacgt tgccgtcggc gtcagtcgtc acggtcgtcg
     6541 ccgacgacca ggtgagcgca agcggtgcgg cggtcagatc gagttcggtc gacgcgctac
     6601 ctgtgacgct cgggcgcagc acgtcgaacg gatcagccgc cagatcgacc agatcgtcaa
     6661 gacgaagccg ttcaggtccg ccgctcgcga tgaggcccac cgccacctcg gcgtgaccgc
     6721 tcccggccac cgtcgcgcct gcattgccac cggcatcagc ggtgcccgcg atcgttccgt
     6781 ctgccacaac atcgatcaac agtgcacttt cggccgagag gtagaagccg tcgctgtcga
     6841 cgccgaacac cagcgtgacg gtgaggtcgg ccaacaggtc gaagtcggcg tcgaggtcga
     6901 ccgcgtcggc cagaccggtg agcagcgcag cgtcgtcgtt gaggtcgtcg ccaccgaacc
     6961 cgccagcacg ggccagatcg gcaagcgtga tctcggtacg agcctggagc agatcggtcg
     7021 gcgacggcgg cagcggcgtg ccaccgactc caccggccac ccagtcgatc gtgataccag
     7081 cggcgcgcag cgcggccacc acctccgcga gctcgccgct catcgggcca tacggcgtgg
     7141 ccaccgcgcc gagggcggct tgggttccga acgccgaccc gagctcgtcg gtgaccacgg
     7201 gcagttcgaa cggggccacg ccaagacttg gaaggtcgaa cccgtcgagg tcgaatgccc
     7261 ggccccactc gggcaccgcc gcggccaccg ccgcgcggcc cgcgttcgac tcggctgcaa
     7321 cttcgccctc gggcggcggc tcggcctcgt cgtcgtcgac gatcgtgccc aacgcgcgca
     7381 gcatgttcac gtcgagttgg gcgttgaacg gctccgacaa cgtgacctca accgtacggt
     7441 cgccatcggc gaagtcgttg gggtagatcg acacgctgaa cgtcgccgtg tgcgtgcccg
     7501 gcgggaacat caccacggaa cctcgtgcgt cgtaatcctc gccatccacc gcgctgccgc
     7561 cgtcgctcgt cgcagcaaac acccacacgt ctcgacccga gggtgcggga gcgccgaaca
     7621 ggtcgaccaa ctcgatggga tagaccaact cgcggaaccc gaagttgccc tcgatcacct
     7681 cggtgtcgag aatgcgcacg atcggctcgg ggtcgtcgtc gagaatcgag atcgttcccg
     7741 agaccccacc gacgacgagc cggaaggtct cgtcgttctc gtcgagagcg tcgttgacga
     7801 tcgtgatcgg caccaccaca gaaccattgg gcggtacgac gaccgaaccg acgaacggaa
     7861 gaaagtcggc tggggccgac gccgagctct ccacggttcc cccgggaacg gtcagcgcca
     7921 cgttgccgcc gacgccttgc acgatgaccg ccgcggtgcc cgccgactcc aacacctgct
     7981 ggggcgcgat gacgatcgtc ggcggcacgg gtggtggagt gtcggcgtac acgacggtca
     8041 cgtcgtggtc gccgtagacg acgtcccagc cggtggcgtc gaccgtcgcg aacgtgccgg
     8101 tcacggttcc cgtagccgga gacgcctgga tgaacgcgaa cgggtcggcg tcggaaccca
     8161 ggggctgcat ggccacgtgc agatggccgc cgagtgtgat ctggccgggc acgataatct
     8221 cgccaaggta cgacggatcg ctcatcgcga gcaccgacgt ctcgtccaga cggaggtgcc
     8281 ccgcgaccgt caagaaggcg aggaaggcga tctcgatggt gccagcgttc gtgtgcagcc
     8341 cggggccgag cgtgagcgcg ttgaactgcg gcagccgcag ggtgccagcg ttgtccagcc
     8401 gcactccgct cagcgtgacg aagccgtcgt cgacgtcgac gaccatgaca ccctggttgt
     8461 gcacgacagg ctcggccgtc gagtcctccg tgtcggtccc gatgttgcca ccgatgcgcc
     8521 actctgcgcc cgggtgaatg gtgaaggtgc cgtcgtggat tcccgtatag ccctcggtac
     8581 gcagggtgcg atgtaggtcg tagcgcttgg agttgtcgat cgacgagcgc gggtcgaggt
     8641 acgtcgaatc ggtgaccgtc aacgtgcccg acc
//
LOCUS       DAORNF010000027         8649 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_6052_length_8649_cov_1.718408, whole genome shotgun sequence.
ACCESSION   DAORNF010000027 DAORNF010000000
VERSION     DAORNF010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 8649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8649
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6052_length_8649_cov_1.718408"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>411
                     /locus_tag="PLV68_02000"
     CDS             <1..>411
                     /locus_tag="PLV68_02000"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRE00039.1"
                     /translation="IATEPLPAATWDAIGLAQRETFYDARHVVIYGQRTADDRIAFGG
                     RGAPYHFASAVRPEFDRVDRVHEMLRDTLVGLFPAVADAVITHRWGGPLGVPRDWHAG
                     VGIDHATGLAWAGGYVGDGVATTNLAGRTLADLIL"
     assembly_gap    412..421
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <422..614
                     /locus_tag="PLV68_02005"
     CDS             <422..614
                     /locus_tag="PLV68_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00040.1"
                     /translation="GRESDLVSLPWVGHRSRAWEPEPLRWLAIQSSLWLSGNIDRAER
                     RRGRPPRWRSAILDRLTGG"
     gene            complement(652..1395)
                     /locus_tag="PLV68_02010"
     CDS             complement(652..1395)
                     /locus_tag="PLV68_02010"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRE00041.1"
                     /translation="MLFGAVGGDVVVAVAGVILFASLVTAVAGFGFALMSVPLMSLVI
                     DIHTAVVVSSMVSVCANGVQAFVDRRYRDGTLIRRLTLACFIGLPFGYLVYRAVGDTA
                     LRIVLGAGVIVAVALLSRGLNLTHVGPGLDWAMGIISGVLSTSISTSGPPLVFDLQAR
                     RLDPNVFRATIQAVFLFAGLAGLVLFSVGGDIGRDELAISAAAVPALAAGLLLGFPLR
                     RRVTGDRFRVLVNVLLTVSAVAAVSKAFT"
     gene            complement(1475..>2276)
                     /locus_tag="PLV68_02015"
     CDS             complement(1475..>2276)
                     /locus_tag="PLV68_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRE00042.1"
                     /translation="GSLGPQIDPALTTRVVPFNDIDALDAALAHGDVACVLMEPALTN
                     IGIVLPQPGYLEAVRERTRAAGALLVLDETHTICVGPGGAGAAWGIEADFVVVGKTIG
                     GGVPAAAYGMSAEIAARLERPVNDDSIDTSGVGGTLTGNALALAAVRATLSNCLRDED
                     FAVAVPLADAWTDDVAKVIADAGLPWHVQRLGCRGEYWFCPPPITGRDAALAIDHELD
                     ALMHLWAINRGILLTPFHNMALLSPFHTQADIDLHTAVFADAVAALTT"
     assembly_gap    2277..2286
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2287..2690)
                     /locus_tag="PLV68_02020"
     CDS             complement(<2287..2690)
                     /locus_tag="PLV68_02020"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRE00043.1"
                     /translation="MTRWAGEFPLYVSEAYGGHFSDVDGNRFVDFCLGDTGAMVGHAL
                     AQVAEAVAAQARRGITTMLPSEDGAWVGAELARRFGLPVWQMAMTATDANRFVLRFAR
                     HLSARPKVIVMDWCYHGTVDETFAVLDDNGRV"
     gene            2997..3437
                     /locus_tag="PLV68_02025"
     CDS             2997..3437
                     /locus_tag="PLV68_02025"
                     /inference="COORDINATES: protein motif:HMM:NF013231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRE00044.1"
                     /translation="MATPAGIVDRATLTIARLHRALEHTPDTELTLPHYRVLGLLAAG
                     DERASVLASRLAVSRPTVTALVDSLVERGFVTREPGTSDRRSITVSITADGRAAVAAT
                     GVALRATLDAVLAGCADRTVVDAALEQLAPALDLWFTARTAAKG"
     gene            3581..4651
                     /locus_tag="PLV68_02030"
     CDS             3581..4651
                     /locus_tag="PLV68_02030"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRE00045.1"
                     /translation="MIVALVFSVVAQALIGLIPLIQQHIVDHAIVAHDTRVGPWLVVL
                     LVTGVAGFSANFGRRYLGGKVTVDIQHDIRRAIHRRLYELDFATHDRLSVGDVMSRAA
                     GDLTLVTQFLFQIPMLAAQGTLLVVAVGVMLSLSPLLSLVVLAVVPLFVFVAVRFRNR
                     VFPASWNDQRLSGLVAGVVDEAVNGVRVVKAFAQEDRELTRLTDRANELYQSRMRTAR
                     INAIYSSTLQALPMLGQVGVLAVGGWLALNGHITIGVFLAFASYLVQIITPVRLMSSM
                     LASAQQARAGSQRLFDLFDLEPIGRQTRGARRGCQRPTRWRDHSRSCQLWARRHWSRR
                     QRRRGSAEPPVAARHITHDPPW"
     assembly_gap    4759..4785
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4786..>5452
                     /locus_tag="PLV68_02035"
     CDS             <4786..>5452
                     /locus_tag="PLV68_02035"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRE00046.1"
                     /translation="SLRRQVSVVFEESFLFSTTIAENIAFGRPDATRDEIVAAAKVAC
                     AHEFISSLPDGYDTAVGERGFTLSGGQRQRIALARAALLDPAVLILDDATSAIDAQTE
                     EAIFAALAHRLGARTTVLIAHRSSTLRLADRVLVVDDGRIVADGTDAELWHTSRLYRE
                     LLTGPDLGEEPADTSAVTAPTTAAWPYPDGDGPGHGGTHEIQMALLLGDQTVGGGVAV
                     GGGV"
     assembly_gap    5453..5637
                     /estimated_length=185
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5638..>5916
                     /locus_tag="PLV68_02040"
     CDS             <5638..>5916
                     /locus_tag="PLV68_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HRE00047.1"
                     /translation="AGAFVVIDAATTLVGPLLIRHGLGTGVSAGQHRVLWEMVAAFLV
                     VQLVSWVNQSIELRSVSRTGERMLYTLRLRTFAHLQRLSLDYYDRELGG"
     assembly_gap    5917..6000
                     /estimated_length=84
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6001..7348
                     /locus_tag="PLV68_02045"
     CDS             <6001..7348
                     /locus_tag="PLV68_02045"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRE00048.1"
                     /translation="CGGVVAVLLILDWRLALAALAVLPALAAVTVWFQRESNRAYVRA
                     REAVSATNAELQESIAGVRVTQALGRTDNNADRFASKSWAFRTARLRSMHLMGIYFGA
                     SQFLSTVATALTLWYGAHLIDKGSITPVLLLAFLLYLQQFFTPLQQLSAVFDQWVQAG
                     ISLGRLDELLATPSSTPLPEHPIDPGQWSGEIVLENVRFRYSPAAPEALRGVDLTIAP
                     GEMVALVGTTGAGKSTFVKLVARFYDPTGGRVLADGLDLRSLDLRSYRRHLGYVPQEP
                     FLFAGTIASNIAYGRPEATDLEIERAGRAVGAHDFVAAMPQGYLTPVAETGRSLSAGQ
                     RQLLCLARAALVDPTILILDEATSNLDLASEAEVQRAMARAAAGRTTLLIAHRLQTAR
                     RADRIVVIDEGQIAESGSHDDLLAQGGRYAALWDTFDTARTSQGQPPRSERRTPPG"
     gene            7451..>7829
                     /locus_tag="PLV68_02050"
     CDS             7451..>7829
                     /locus_tag="PLV68_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00049.1"
                     /translation="MGSSVADRAVSVAPPAPRAHLAGVTVYQPGKSAEEAMAEHGIDS
                     AVKLASNELPFGPLPGVTEAVIEAMATTDRYADHISDRLATAFADLVGVRREQVAVGP
                     GSVGLLQQIALAYAGPGTEVVYPW"
     assembly_gap    7830..7918
                     /estimated_length=89
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7919..>8278
                     /locus_tag="PLV68_02055"
     CDS             <7919..>8278
                     /locus_tag="PLV68_02055"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRE00050.1"
                     /translation="ITPATTLVFIAQPNNPISDALRTAELERLVAGVPDQCLLVIDEA
                     YREFVTGADVPDAMTYLDRHPNLVVLRTLSKAYGMAAMRVGFAVGDPHVIAAINATLI
                     PFAVNGPAQAAALVALQ"
     assembly_gap    8279..8293
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8294..>8649
                     /locus_tag="PLV68_02060"
     CDS             <8294..>8649
                     /locus_tag="PLV68_02060"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00051.1"
                     /translation="RRCAVVTAERDAGAQRLRRSGFSLPASQANFWWLPAGERATDLA
                     GALERRGVVVRPFPTGVRVTVSTPDENARFFAAFEAVVGSDPGYSEAWQAPTGDAARA
                     VADLLDALAAPCPDGA"
BASE COUNT         1251 a         2841 c         2796 g         1341 t
ORIGIN      
        1 gatcgcgacc gaaccgttgc ccgcagcgac gtgggacgcg atcggcctcg cccagcggga
       61 gacgttctac gacgcccgtc acgtcgtgat ctacggccaa cgcacggccg acgaccggat
      121 cgcgttcggc ggccgcggcg cgccgtacca cttcgccagc gcggtgcgtc ccgagttcga
      181 tcgagtcgac cgcgtccacg agatgctgcg cgacaccttg gtcgggctgt ttccagcggt
      241 cgccgacgcg gtgatcacgc atcgctgggg tgggccgctc ggcgtgccgc gcgactggca
      301 cgccggggtc ggcatcgacc acgcgactgg cctcgcctgg gccggcgggt acgtcggtga
      361 cggcgtcgcg acgaccaacc tggccgggcg aacgctggct gatctgatcc tnnnnnnnnn
      421 ncggcaggga gagcgacctg gtgtcgctgc cgtgggtcgg acatcggtcg cgcgcgtggg
      481 agcccgagcc gttgcgctgg ctcgcgatcc agtcgtcgct ctggctgtcc ggcaacatcg
      541 accgcgccga acgccgccgt ggccgcccgc cccgctggcg tagtgcgatc ctcgaccgtc
      601 tcaccggcgg ctgaccagac tgtctcgatg tcgaggcgtt gcgccgccgc gtcaggtgaa
      661 ggccttcgac acggcggcga cggcgctgac ggtgagcagc acattgacca gcacccggaa
      721 gcggtcgccg gtgacgcggc gccgcaacgg gaagcccaac agcaacccgg ctgccagcgc
      781 tggtaccgcg gccgcgctga tcgccaactc gtcgcgcccg atgtcgccgc ccacgctgaa
      841 caacaccaga ccggccaggc ccgcgaacag gaacacggcc tggatcgtgg cccggaacac
      901 gttcggatcc agtcgccggg cctgcaggtc gaacaccagg ggcggcccgc tggtcgagat
      961 cgacgtgctc agcacgccgc tgatgatgcc catcgcccag tccaaccccg gcccgacgtg
     1021 ggtcaagttg agcccgcgcg acagcagtgc cacggccacg atcacccccg cgccgagcac
     1081 gatccgcaac gccgtgtcac cgacggcgcg gtacacgaga tagccgaacg gcagcccgat
     1141 gaagcacgcc agcgtcagcc gccggatgag ggtgccgtcg cggtagcggc ggtcgacgaa
     1201 ggcctgtacg ccgttggcgc acaccgacac catcgacgac acgacgaccg cggtgtgaat
     1261 gtcgatcacc agactcatca gcggcaccga catgagcgcg aagccaaacc ccgccaccgc
     1321 cgtgaccaac gacgcgaaca agatcacccc cgccaccgcg accaccacgt cgccccctac
     1381 cgcgccgaac aacatcaacg ccacccccgc ctcacgcccc gcaactctac ggccgtgccc
     1441 acccggcgct ggggaggatt cgggcgctga ggagtcaggt ggtgagggcg gcgacggcgt
     1501 cggcgaagac ggcggtgtgg aggtcgatgt cggcttgggt gtggaacgga gacagcaggg
     1561 ccatgttgtg aaaaggggtg agcaggatgc cgcggttgat cgcccacagg tgcatcagcg
     1621 cgtcgagctc gtggtcgatc gcgagcgccg cgtcgcggcc ggtgatcggc ggcgggcaga
     1681 accagtactc gccgcgacag ccgagccgct gcacgtgcca cggcagaccg gcgtcggcga
     1741 tcaccttggc cacgtcgtcg gtccacgcgt cggccagcgg taccgcgacc gcgaagtcct
     1801 cgtcgcgcaa gcaattcgac aacgtggccc gcaccgccgc gagcgccagc gcgttgccgg
     1861 tgagggtgcc gccgacgcca gacgtgtcga tcgagtcgtc gttgaccggg cgctccaacc
     1921 gggccgcgat ctcggccgac atgccgtaag ccgcggcggg gacgccacca ccgatcgtct
     1981 tgccgacgac gacgaagtcg gcctcgatgc cccacgccgc gccagccccg cccggtccga
     2041 cgcagatcgt gtgcgtctcg tccagcacca acagcgcgcc agcggcgcgg gtgcgttcgc
     2101 gaaccgcttc cagatagccc ggctgcggca acacgatgcc gatgtttgtc agtgccggtt
     2161 ccatcaagac gcacgcgacg tcgccgtggg cgagcgctgc gtcgagcgcg tcgatgtcgt
     2221 tgaacggcac gacacgagtg gtcagcgcgg ggtcgatttg cgggccgagc gaacccnnnn
     2281 nnnnnnatga cccggccgtt gtcgtcgagc accgcgaacg tctcgtcaac cgtgccgtgg
     2341 tagcaccaat ccatcacgat caccttgggg cgagcggaga ggtgacgggc gaagcggagc
     2401 acgaatcgat tggcatcggt ggcggtcatc gccatctgcc acacgggcag gccgaagcgt
     2461 cgggcgagct cggcgccgac ccacgcaccg tcctcgctgg gcagcatcgt ggtgatgccg
     2521 cggcgtgcct gagcggcgac cgcttcggct acctgcgcca gggcgtgacc gaccatcgcg
     2581 ccggtgtcac ccaggcagaa gtcgacgaag cggttgccgt cgacatcgga gaagtgcccg
     2641 ccgtatgcct cgctgacata cagcgggaac tccccggccc accgtgtcat ccacggcatg
     2701 ggcaccccgc cgagcagatg cgtcgccccg tcccgtcgcg cccgcgagtt cggatgccgc
     2761 tcgcgatacc gccgatcctc catggctctc agcgatcgca ctcgctcgcg atcgatccga
     2821 cgcatacgat caatctaagt ccgccatagc gagtggtcaa ctgattcaat cgaatcaaga
     2881 gtaaatgacg attgtttcgc ttgtatccgg gctactcgga gcgatgatcg tgctgcgtcc
     2941 ctgagtgttg gatcctgatg tggttcacag tttgaattat tcggtaggct aacgaaatgg
     3001 ctactcctgc tgggatcgtc gatcgcgcca cgctcacgat cgcccggctc caccgcgccc
     3061 tggaacacac gccggacacg gagctgacgc tgccccacta ccgcgtgctc ggcctgctcg
     3121 cggcaggcga cgaacgcgcc agcgtcctcg cctcgcgttt ggcggtcagc cgccccaccg
     3181 tgacggcgct ggtcgacagc ctggtcgagc gtggcttcgt cactcgcgag ccgggcacga
     3241 gtgaccgccg ctcgatcacc gtgtcgatca cagccgacgg acgggctgca gtcgcggcga
     3301 ccggggtcgc cctgcgcgcg actctcgacg cggtgctcgc cggttgcgcc gaccggaccg
     3361 tggtcgacgc ggcgctggaa cagctcgcac ccgctctcga cctgtggttc actgcgcgga
     3421 ctgcagccaa agggtgaaaa cgctgagcgc caatggcgcg gccagcgggt cgtcgagctc
     3481 gacccgcggc gacgcctccg cggtccgccc cgccccacct ggtgatggcg tcgggtggtt
     3541 gcgtcgcttg tggccgttca tgcgccgcca ccggggcacc atgatcgtcg cactggtgtt
     3601 ctcggtcgtc gctcaggcgt tgatcggctt gattccgctc atccaacaac acatcgtcga
     3661 tcacgcgatc gtcgctcacg acacacgtgt cgggccgtgg ctggtcgtgc tgctggtcac
     3721 cggagtcgcc gggttcagcg ccaacttcgg ccgtcgctac ctcggcggca aggtcactgt
     3781 cgacatccag cacgacatcc gccgggcgat ccaccgccga ctgtacgaac tggacttcgc
     3841 gacccatgat cggctctcgg tcggtgacgt catgtcgcgt gcggcgggtg acctcacctt
     3901 ggtcacccag ttcctgttcc agatcccgat gcttgctgcg cagggcacgc tgctggtcgt
     3961 tgccgtcggc gtgatgctgt cgttgtcgcc gttgctgagc ttggtggtcc tggccgtcgt
     4021 accgctgttc gtgttcgtcg ccgtgcgctt ccgtaaccgg gtgttcccgg cgagttggaa
     4081 cgatcagcgc ctgtctggat tggtggcagg ggtggtcgac gaggcggtca acggagtgcg
     4141 ggtggtcaaa gccttcgctc aggaagaccg tgagctgacc aggctgaccg accgggccaa
     4201 cgagctctac cagtcgcgga tgcgcaccgc gcgcatcaat gcgatctact cctcgacgtt
     4261 gcaggcgctg ccgatgctgg ggcaggtggg tgttctggct gtcggagggt ggctggcgct
     4321 caacggccac atcacgatcg gcgtcttctt ggctttcgcc agctacctcg tgcagatcat
     4381 cactccggtc aggttgatgt cgtcgatgct tgcgtccgcc cagcaggcgc gggctgggtc
     4441 gcagcgactg ttcgacctgt tcgacctgga gccgatcggt cgacagaccc ggggcgcgcg
     4501 tcgcgggtgc caacggccga ctcgctggcg agatcactct cgatcatgtc agctttgggc
     4561 acgccgacac tggtcacgcc gacaacggcg gcgaggctcg gccgaaccgc ctgttgctgc
     4621 gcgacatatc actcacgatc cgccctggtg agcggatcgg catcgtcggg gcgtcggggt
     4681 cgggcaagac caccctcgcg ttcttgttgg cccggttcta cgaaccgacc tcggggacgg
     4741 tgtctctcga gggcgtcgnn nnnnnnnnnn nnnnnnnnnn nnnnngtcgt tgcgtcgtca
     4801 ggtcagcgtg gtgttcgagg agagctttct gttctccacc acgatcgccg agaacatcgc
     4861 cttcggccgt cccgatgcga ctcgggacga gatcgtggcc gcggccaagg tggcctgcgc
     4921 ccacgagttc atcagcagcc tgccggacgg gtacgacaca gccgtcggcg agcgcgggtt
     4981 cacgctgtcg ggtggccaac gccagcggat cgcactggct cgggcggccc tgctcgaccc
     5041 agccgtcttg atcctcgacg acgccacgtc ggcgatcgac gcccagaccg aagaggcgat
     5101 cttcgccgct ctggcccatc ggctgggtgc ccgtaccacc gtgctgatcg cccaccggtc
     5161 gtccaccctg cggctcgccg atcgggtgct ggtggtcgat gacggacgaa tcgtcgccga
     5221 cggcaccgat gccgagctgt ggcacacctc acggctctat cgcgaactgc tgaccggtcc
     5281 cgatctgggc gaggaaccgg ccgacacgag tgccgtcacg gcgcccacga cggcggcctg
     5341 gccctacccc gatggcgacg ggccgggcca cggcggcact catgagattc agatggcgct
     5401 gctgctcggc gaccagaccg tcggcggagg cgtcgcggtc gggggtggag tgnnnnnnnn
     5461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngcc
     5641 ggcgcgttcg tggtgatcga tgccgcgacc acccttgtcg gcccgctgct gatccgccac
     5701 gggctcggta ccggtgtgtc cgctggccaa catcgtgttc tgtgggagat ggtggcggcg
     5761 ttcctggtgg tgcaactcgt cagctgggtc aaccagagca tcgaactgcg gtccgtgtcg
     5821 cgcaccggtg agcgaatgct ctacacactc cgcctgcgca cctttgccca cctgcaacga
     5881 ctgtcgctcg actattacga ccgcgagctc ggcgggnnnn nnnnnnnnnn nnnnnnnnnn
     5941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6001 gtgcggcggc gtggtcgcag tgttgctcat actcgactgg cgtttggcgc tggccgcgct
     6061 ggccgtgttg ccagccctcg ctgcggtcac cgtgtggttc cagcgcgagt cgaaccgagc
     6121 ctacgtgcgg gcacgcgaag cggtgtcggc caccaacgcc gagttgcagg agagcatcgc
     6181 tggcgtgcgc gtcacccagg cgctcggacg cacggacaac aatgccgacc ggttcgccag
     6241 caaatcgtgg gcgttccgga cggcgcgcct gcgatcgatg cacctgatgg gcatctactt
     6301 cggcgctagc cagttcctca gcacggtcgc cactgcgctc acactgtggt acggcgctca
     6361 cctgatcgac aaggggtcga tcacgcccgt gttgttgctg gcgttcctgc tgtacctgca
     6421 gcagttcttc accccgctcc agcagctctc cgcggtgttc gaccagtggg tgcaggcggg
     6481 tatctcgctg ggtcgactcg acgaactgct ggcgacgccg tcgtcgacgc cgctccccga
     6541 gcatccgatc gatcccggcc agtggtcagg cgagatcgtc ctcgagaacg tcaggttccg
     6601 ctactcgcca gccgcgcccg aggccctgcg cggggttgac ctcacgatcg ctcccggcga
     6661 gatggtcgcg ttggttggca cgactggagc cggcaagtcg accttcgtca agctcgtggc
     6721 acggttctac gacccgaccg gtggacgggt gttggccgac gggctcgacc tccgctcgct
     6781 cgacctgcgc agctatcgcc gtcacctcgg gtacgttccc caggagccgt tcctgttcgc
     6841 tggcacgatc gcatcgaaca tcgcctacgg acggcccgag gcgaccgatc tggagatcga
     6901 gcgtgcgggg cgggcggtcg gcgcccatga cttcgtggcc gcgatgccac agggctatct
     6961 caccccagtc gccgagaccg ggcgatcgct gtcggcgggt caacgccagc tcctgtgctt
     7021 ggctcgggcg gccttggtgg acccgacgat cctgatcctc gacgaggcga cctcgaacct
     7081 cgatctggcc agcgaagccg aagtgcagcg ggccatggcg cgcgccgcgg ccggacgcac
     7141 cacactgttg atcgcccatc gcctccagac ggcgcggcgc gccgaccgga tcgtcgtcat
     7201 cgacgaaggc cagatcgccg agtccggctc acacgacgat ctgcttgccc agggtggccg
     7261 ctacgccgcc ctgtgggaca ccttcgacac agcccgcacc agtcagggac agccgccacg
     7321 gtccgagcgg cgaacacctc ccgggtgaac ggcacgaaga gctgtggtcg tgacccgcgg
     7381 tcggacccga cgatcgcggg tggctgttcc aggctgccga ctggaggcgg cgagatggca
     7441 ggctctgggg atgggttcgt ccgtcgctga tcgtgctgtg tccgttgctc cgcctgcgcc
     7501 tcgggcgcac ttggcggggg tgacggtgta ccaacccggc aagagcgcgg aggaggcgat
     7561 ggccgagcac ggcatcgact cggcggtcaa gctggcatcg aacgagttgc cgttcggacc
     7621 gctgcccggg gtgaccgagg cggtcatcga ggcgatggca acgaccgacc gctacgccga
     7681 tcacatctcc gatcggttgg ccaccgcgtt cgcggacttg gtcggtgtgc gccgcgagca
     7741 ggtcgcggtc gggcccggtt cggtcggact gctgcagcag atcgccttgg cctacgccgg
     7801 acccggcacc gaggtggtct atccctggcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncg
     7921 atcactcctg cgaccacgct ggtgttcatc gcccagccca acaacccgat ctccgacgcg
     7981 ctgcgcacgg ccgagctcga gcgcttggtc gccggtgtcc ccgaccaatg cctgctcgtg
     8041 atcgacgagg cctaccgcga gttcgtcacc ggtgcagatg ttccggatgc aatgacctat
     8101 ctcgatcgcc acccgaacct ggtcgtgctg cgcacgctgt cgaaggcgta tggcatggcg
     8161 gcgatgcggg tcggcttcgc cgtcggcgac ccgcacgtga tcgcggcgat caacgcgacg
     8221 ctgatcccgt tcgccgtcaa cggaccagcg caggctgcag cgctggtggc gttgcagcnn
     8281 nnnnnnnnnn nnncccggcg ctgcgcggtg gtcaccgccg agcgcgatgc gggcgcgcaa
     8341 cggttgcgcc gcagcgggtt cagcctgccc gcgtcgcagg ccaacttctg gtggttgccc
     8401 gcaggcgaac gggctaccga cctggccggg gcgttggagc gacgtggcgt cgtcgtgcgt
     8461 ccgttcccga cgggggttcg ggtcacggtg tcgactcccg acgagaacgc acggttcttc
     8521 gccgcattcg aagcggtggt tgggagcgac ccgggataca gcgaggcgtg gcaagcgccg
     8581 acgggcgacg ccgcccgggc cgtggccgac ctgctcgatg cgcttgccgc gccctgcccg
     8641 gacggagcc
//
LOCUS       DAORNF010000028         8441 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_6210_length_8441_cov_1.789292, whole genome shotgun sequence.
ACCESSION   DAORNF010000028 DAORNF010000000
VERSION     DAORNF010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 8441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8441
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6210_length_8441_cov_1.789292"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /locus_tag="PLV68_02065"
     CDS             <1..465
                     /locus_tag="PLV68_02065"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRE00052.1"
                     /translation="WSRGAWLGAAAGVVVVLVVRSKQAAIASALVALHLDVPARRVVL
                     GAIDADAQWCTSALTLVESLALIDRVTDQDVLRSDLEDLVRLSWDRLAVVPVDQPCLD
                     RAAALMRRQPLRLSDALHLAAADRLPRPVRFVTFDPAQIPVALSMEFDVIST"
     gene            519..2837
                     /locus_tag="PLV68_02070"
     CDS             519..2837
                     /locus_tag="PLV68_02070"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HRE00053.1"
                     /translation="MGRTGRAVRWAAQQVRSTGATARQHGWRAAIGERIAAAAAASPV
                     HRQATTQRDQRIAAVEGRADHFQHHLHLVDSRFDDSDVRQHELAAALRNEIGEAHAGI
                     TEHIMRTAADTETIRENYGSHLRSLQRQVDDLHRDTDRLQRAASSLNHIVTDWLWAAN
                     APLPDHPLISVVMATAFPERISYLRSAIDSVLAQSYPNWELIVIDDGDEPFLDPAPAW
                     WPTDPRVRLLRGEGRSEGRARNAGIAAVTGAVVAYLDDDCRWFPWWLHAFARVFASEP
                     DIGVVHGIRIIEADEINPTWSYAQELSALTLQTGNPADTNVMAHRAGLPDGGWPRIAS
                     CADYDTAIRLVGSGVRYIPVPAVAYATSSPSRTWAPERAEINAANFNLVQRRARLARP
                     LRVVAHNSLYPLLSETYIGDELAALQQRDIDVVLSRVSPASVPTVSRIDAPQFESLED
                     AIAAHDPDVVLSHWAGVGLEMRKIAGRARVPYGIRLHGFDGGYPLHDLIDEWCVGLWD
                     FAHRGGRHHLQFSLDTLIADPPPFSDAPRSGRVMSLSAGLPKKDFPRLIAAIGQLGDD
                     VHLDIVTAVTNGFETMPAEVRGEAKAVGLRDRITVAENVDYHLAQAQLTAAGEELLRE
                     GRRLLCQMDAVANRVLALPLIVRAGRLVVADGAPLSTWRHRLAGGGHRQRADRSVQRR
                     RAPRPVRARPRTAQPSPGDRRLGQLVHQRRRHMAASPPHRPRRGQSALVVGVIGPLVG
                     RDHHRERAGRVSGAAGVDEAVAGMRNADHSAR"
     assembly_gap    2895..2927
                     /estimated_length=33
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2928..3792)
                     /locus_tag="PLV68_02075"
     CDS             complement(<2928..3792)
                     /locus_tag="PLV68_02075"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HRE00054.1"
                     /translation="MASSGMAGLRLGEMSPAPEALPVVTPNDRIRADGRPQPGFRDEL
                     RRIPSAANAASVVGLYVQTAVLLTAVVVTPGWWRLLVWPVVFVLMGRTHAQFASLMHE
                     AAHRLLFAKKSLNDWVGRWLLGYPAFTSTEAYRRVHMAHHREEFGPDEPDIPLYVNYP
                     VSAASLRRKLVRDATGQTGWKLLKQQLRGLANPNRAARRVQYKIWTVQVVLLALSIVF
                     GVWYLYPVFWLLPYLTVWRVINRLRSIAEHGGMMASKDRRITTHSVRQHRVARFLLVP
                     YNIGWHLAHHVD"
     gene            complement(3804..4418)
                     /locus_tag="PLV68_02080"
     CDS             complement(3804..4418)
                     /locus_tag="PLV68_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRE00055.1"
                     /translation="MTTLHWSDSRLEVHKLVVGPYDNNVFVLRCRETGDAVMIDAANE
                     HDKLTELCRTLGVTRVLETHGHWDHIQAVPAMREMGLQVAVASADAPMLRDVGYDLFL
                     DDAEVIEVGRLRLHAIHNPGHTPGSISFKVEGAPVLFSGDTLFPGGVGNTKTKEGDFA
                     TIIQSVDSRLFSLPADTVVMPGHGVDTTIGAERPFLDAWVERGW"
     gene            4539..5315
                     /gene="sufC"
                     /locus_tag="PLV68_02085"
     CDS             4539..5315
                     /gene="sufC"
                     /locus_tag="PLV68_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012726854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HRE00056.1"
                     /translation="MTELFRIERLRARPVEGDTDILRGVDLTVAEGEVHAIMGPNGSG
                     KSTLASTLLGSPEYDVTAGQLFLRGDDITDWPTDLRAKSGMFLGFQYPQEIPGVSVIQ
                     FLRQALSARKGIDLSVLELRLATMEWMKRLGMDSSFVDRYLNEGFSGGEKKRNEIMQM
                     AILEPELAILDETDSGLDIDALRIVAKGIREVKADRPALGIVLITHYQRLLDELQPDW
                     VHILIDGRIVASGGMELAERLEADGYESFRDASRAAASIG"
     gene            5329..>5585
                     /locus_tag="PLV68_02090"
     CDS             5329..>5585
                     /locus_tag="PLV68_02090"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRE00057.1"
                     /translation="MTTASSDIFSTETLDVARIRADFPIFGAAAQQWRSEDQRLVYLD
                     SANTSQKPSQVLDAMNTFAATAYGPINRSAYRLAAEATDAF"
     assembly_gap    5586..5635
                     /estimated_length=50
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5636..6608
                     /locus_tag="PLV68_02095"
     CDS             <5636..6608
                     /locus_tag="PLV68_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRE00058.1"
                     /translation="IVFTKNATEALNLVVQSWGRANLASGDVVVLTHMEHHANIVPWH
                     ILAAERGIQLRWVPLTDDGQLDLTDLDALLDGAKAFAFTAMSNVLGTIPPIARLCAAA
                     HRAGALAIVDGCQYVPHNVTDVQAWDADFLAFSSHKMCGPSGIGALWGRAELLDAMPP
                     FLGGGNMIADVRLDGFTTAPVPAKFEAGTPAIIEAIGFGAAVDYLTGLGMANVRRHEM
                     EMAAYAIDSLTQRYGDDVTIHGPRNVEVRGATLSFAFRDIHPHDISQVLDQRNVCVRA
                     GHHCAKPLMRLLGANATVRASFYVYNDSADADSLTDALEGASDIFGL"
     gene            6642..7103
                     /locus_tag="PLV68_02100"
     CDS             6642..7103
                     /locus_tag="PLV68_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HRE00059.1"
                     /translation="MPGLEDLYREIILDHYRSPRNRGELDPPAVRVDGHNPLCGDEIQ
                     LYLAVDDGVVTDIKVGGSGCSISQSSASMMTQAVKGKPIAEVKALVRRFKGMMSVDEI
                     DDDPAAEAAAAEVKLGDLEALQGVVKFPVRIKCATLAWNTLLQAIDQTTSA"
     assembly_gap    7448..7457
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7458..>7741
                     /locus_tag="PLV68_02105"
     CDS             <7458..>7741
                     /locus_tag="PLV68_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00060.1"
                     /translation="RSRDNTGFHDRWPAIERDGDPLPTYGDRPFSGVSSPWSVEPDVV
                     RFGPGVRATVTFGAAHEGAPGRCHGGIIAGLFDDLLGSAVAVLGVAVFTG"
     assembly_gap    7742..7858
                     /estimated_length=117
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(8035..8265)
                     /locus_tag="PLV68_02110"
     CDS             complement(8035..8265)
                     /locus_tag="PLV68_02110"
                     /inference="COORDINATES: protein motif:HMM:NF013561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HRE00061.1"
                     /translation="MPVIDAQIAAWGDEAEVGYDIGVLKKRGKGRPGRGAEPSQVVAL
                     RLTTAEIAALDARAQREGKSRSEVIRDALALA"
BASE COUNT         1354 a         2938 c         2670 g         1269 t
ORIGIN      
        1 tggagtcgtg gcgcctggtt gggggcggcg gctggcgtcg tggtggtgtt ggtggtgcgc
       61 agcaagcaag ccgccatcgc gagcgcgctg gtggcgctgc acctcgacgt gcccgcccgc
      121 cgcgtcgtgc tcggcgcgat cgacgccgac gcgcagtggt gcaccagcgc gctgaccctg
      181 gtcgagtcgc tggcgctgat cgaccgggtc accgaccagg acgtgctccg cagcgatctc
      241 gaagacctgg tccgcctgag ctgggatcgc ctcgccgtcg tgccggtcga tcagccatgc
      301 ctcgatcggg ccgcagcgct gatgcggcga cagccgctgc gcctcagcga cgccctccat
      361 ctcgcggccg cggatcgcct cccccgcccg gtgcgcttcg tcaccttcga tccggcccag
      421 ataccggtcg cgctgtcgat ggagttcgac gtcatctcca cctgatcgca acgtggtgca
      481 aggcgccacc aacacatcac ccagctacgg ttttgaccat gggcaggacg ggacgggcgg
      541 tgcggtgggc ggcccaacag gtacgcagca ccggcgcgac agcgcgccag catggttggc
      601 gggcagcgat cggcgaacga atcgcagctg cggcagcagc ctcaccagta caccgccagg
      661 cgacgaccca acgcgaccaa cgaatcgccg cggtcgaggg ccgcgccgat cacttccagc
      721 accacctcca tctggtcgac tcacgcttcg acgattccga cgtgcgccag cacgaactcg
      781 cagccgcgct gcgcaacgag atcggggagg cgcatgcggg catcaccgaa cacatcatgc
      841 ggaccgctgc agataccgaa acgatccgcg agaactacgg cagccacctg cgctcactgc
      901 agcgccaggt cgacgacctg caccgggaca ccgatcgcct ccagcgtgcg gcgagcagtc
      961 tcaaccacat cgtcaccgac tggttgtggg cggccaatgc gccgctgccc gaccatcccc
     1021 tgatcagcgt cgtgatggcc acggcgtttc ccgaacggat ctcgtacctg cgcagcgcga
     1081 tcgacagcgt gctcgcccag tcgtatccca actgggagct gatcgtgatc gacgacggtg
     1141 acgagccgtt cctcgacccg gcacccgcat ggtggccgac cgacccgcga gtccgtctgc
     1201 tccgtggcga ggggcgcagc gaagggcgcg cccgcaacgc cggaatcgcc gcggtcaccg
     1261 gtgcggtcgt ggcctatctc gacgacgact gccgctggtt cccgtggtgg ctgcacgcgt
     1321 tcgcacgcgt gttcgcgtcc gagccggaca tcggcgtcgt ccacggcatc cgcatcatcg
     1381 aggccgacga gatcaacccc acctggtcgt acgcccagga gctgagcgcg ctcacgctgc
     1441 aaaccggcaa tcccgccgac accaacgtca tggcccaccg cgccggactc cccgacggcg
     1501 gttggccgag gatcgcctcg tgcgcggact acgacacggc gatccgcctg gtcggctcgg
     1561 gcgtgcgcta catcccggta cctgctgtcg catacgcgac cagttcgccg tcgcggacgt
     1621 gggcacccga gcgggccgag atcaacgcgg ccaacttcaa cctggtgcag cgccgcgccc
     1681 ggttggcccg cccgctgcgc gtcgtcgccc acaactcgct ctatccgctg ctgtccgaga
     1741 cctacatcgg tgacgagctg gcggcattgc agcagcgcga catcgacgtg gtgctctcgc
     1801 gggtgtcgcc tgcctcggta ccaacggtca gccgtatcga cgcaccgcag ttcgagtcgc
     1861 tggaggatgc gatcgccgcc cacgatccag acgtcgtgct cagccattgg gccggtgtcg
     1921 gcttggagat gcgcaaaatt gccgggcggg caagggtgcc gtacggcatt cgcctccacg
     1981 gcttcgacgg cggatatccc ctccacgact tgatcgatga gtggtgcgtc gggctgtggg
     2041 actttgccca tcgcggtgga cgccaccacc tccagttctc gctcgacacc ctgatcgccg
     2101 atccgccacc gttcagcgat gcacctcgct ctgggcgggt gatgtcgctc agcgcgggcc
     2161 tgccgaagaa ggacttccct cgtctgatcg cggccatcgg ccaactcggt gacgacgtcc
     2221 acctcgacat cgtcaccgcg gtgaccaacg ggttcgagac gatgccggcc gaagtgcgcg
     2281 gcgaggccaa ggcggttggg ctgagagacc gcatcaccgt cgccgagaac gtcgactacc
     2341 acctcgccca ggcgcagctc accgcggctg gcgaggagct gctgcgcgaa ggccgccggc
     2401 tgctgtgcca gatggacgcc gtggccaacc gcgtgctggc gctgccgctg atcgtgcgtg
     2461 ccgggcggct ggtggtcgcg gacggcgcac cgctatcaac gtggcgacac cgcctcgctg
     2521 gcggcggcca tcgacaacgc gctgaccgat ccgttcagcg ccgacgagcg ccacgccctg
     2581 tccgagcgcg tccgcgaacg gcacagccat ccccgggtga tcgacgcctg ggccaactcg
     2641 ttcaccagcg ccgtcgtcac atggcagcgt cgccgccgca ccgaccgcga aggggtcaat
     2701 ctgcgctggt ggtgggcgta ataggcccgc tggtcgggcg cgaccatcac cgtgagcggg
     2761 ccgggcgagt ctctggtgcg gctggcgtgg acgaagcggt ggccgggatg cgcaacgccg
     2821 accacagcgc ccggtaggtg gggtactcga acgagtcgtc gacgtagcca ctggcccgga
     2881 gtgcctcgtg atagnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnagt cgacgtgatg
     2941 agccaggtgc caaccgatgt tgtacggcac cagcaggaac cgggccaccc gatgctggcg
     3001 caccgagtgg gtggtgatgc ggcggtcttt cgacgccatc atcccgccgt gttcggcgat
     3061 ggaccgcagg cggttgatga cccgccacac cgtcaggtac ggcaacagcc agaacacggg
     3121 gtacaggtac cacacgccga acacgatcga cagcgcgagc aacacgacct gcaccgtcca
     3181 gatcttgtac tgcacccgcc gggcggcccg gttggggttg gccaagccgc gcaactgctg
     3241 cttcagcagc ttccatccgg tctggccggt ggcgtcgcgg accagcttgc ggcgtagcga
     3301 cgcggcactc accggatagt tgacgtacaa ggggatgtcg ggctcgtcgg ggccgaactc
     3361 ttcgcggtga tgggccatgt gcacccgccg gtacgcctcg gtggaggtga acgcggggta
     3421 accgagcagc caccgcccga cccagtcgtt gagcgacttc ttggcgaaca acaagcggtg
     3481 ggccgcctcg tgcatcaggg acgcgaactg ggcgtgggtg cggcccatca acacgaacac
     3541 caccggccac accagcagcc gccaccatcc gggcgtgacc acgaccgcgg ttagcagcac
     3601 cgcggtctgt acgtacagcc ccacgacgct ggccgcgttg gcggcggacg ggatgcggcg
     3661 caactcgtcg cggaagccag gttgcggccg tccgtcggca cggatgcggt cgttgggggt
     3721 caccaccggc agcgcctcgg gcgcggggct catctcgccc aggcgcaagc cagccatgcc
     3781 cgagctcgcc atgtcacggt ccatcaccaa ccccgttcga cccacgcgtc gaggaacggc
     3841 cgctcggcac cgatggtggt gtcgacgccg tggccgggca tcaccaccgt gtcggcgggc
     3901 agcgagaaca ggcgcgagtc gaccgactgg atgatggtgg cgaagtcgcc ctccttggtc
     3961 ttggtgttgc cgaccccgcc ggggaacaac gtgtcgccgc tgaacagcac cggcgcgccc
     4021 tcgaccttga acgagatcga acccggcgta tggccggggt tgtggatcgc atgcaggcgt
     4081 agccggccga cctcgatcac ctcggcatcg tcgaggaaca ggtcgtatcc cacgtcgcgc
     4141 agcatcggcg catcggccga cgcgaccgcg acctgcaggc ccatctcgcg catcgcggga
     4201 acagcctgaa tgtggtccca gtggccgtgg gtctccagca cccgggtcac ccccagggtc
     4261 cggcacaact cggtcagttt gtcgtgctcg ttggcggcgt cgatcatcac cgcgtcaccg
     4321 gtctcacggc agcgcaggac gaacacgttg ttgtcgtacg gaccgacgac cagcttgtgc
     4381 acctccaggc ggctgtcgct ccaatgcaat gtcgtcatgt gtccaagtct gtcaccgccc
     4441 agcggaatcg ccgcacccct gcgcgccggt cgagcgcgcg acatttctcc tacgcgcgta
     4501 gggatattcc gtcccacccc agcggtaggc tcgaaaccgt gaccgagctg ttccgcatcg
     4561 aaaggctccg ggcccgcccg gtcgagggtg acaccgacat cctgcgcggg gtcgacctca
     4621 ccgtcgccga gggcgaggtg cacgccatca tgggccccaa cggcagcggc aagagcaccc
     4681 tcgcctccac cctgttgggc tcacccgaat acgacgtcac cgccgggcag ttgttcttgc
     4741 gcggcgacga catcaccgac tggccgaccg acctgcgcgc caagtcgggc atgttcctcg
     4801 gcttccagta cccgcaggag atccctggcg tgtcggtgat ccagttcctg cgccaagctc
     4861 tcagcgcacg caaaggcatc gacctgtccg tgctggaatt gcgcctggcc accatggagt
     4921 ggatgaagcg gctcggtatg gattccagct tcgtcgaccg ctacctcaac gaggggttca
     4981 gcggcggcga gaagaagcgc aacgagatca tgcagatggc catcttggaa cccgagttgg
     5041 cgatcctcga cgagaccgat tccggcctcg acatcgacgc cttgcgcatc gtggccaagg
     5101 gcatccgcga ggtcaaggcc gaccgcccag cactcggcat cgtgctgatc acgcactacc
     5161 aacgcctgct cgacgagtta cagcccgact gggtgcacat cctgatcgac ggccgcatcg
     5221 tcgcctcggg cggcatggaa ctggccgagc ggctcgaagc cgacggctac gagagcttcc
     5281 gcgatgcgtc ccgcgccgcg gccagcatcg gttgaaagga gatcgatggt gaccaccgcc
     5341 agctccgaca tcttcagcac ggagaccctc gacgtcgccc gcatccgcgc cgatttcccc
     5401 atctttggcg ccgccgcgca gcagtggcgg tccgaggatc agcggctggt ctacctcgat
     5461 tcggccaaca cctcgcagaa gccgagccag gtgctcgacg cgatgaacac cttcgcggcc
     5521 accgcctacg gaccgatcaa ccgcagcgcg taccgattgg ccgccgaggc caccgatgcg
     5581 ttcgannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngatcg
     5641 tgttcaccaa gaacgccacc gaggcgctca acctggtggt ccagtcgtgg ggccgagcca
     5701 acctcgcctc gggcgatgtg gtggtgctca cccacatgga gcaccacgcc aacatcgtgc
     5761 cctggcacat cctggccgcc gagcggggca tccagttgcg ctgggtgccg ctcaccgacg
     5821 acggccaact cgacctcacc gacctcgacg cgttgctcga cggcgccaag gcgttcgcgt
     5881 tcacggcgat gagcaacgtg ctcggcacga tcccgcccat cgcccggctg tgcgcggccg
     5941 cccatcgcgc gggcgctctc gcaatcgtcg acgggtgtca gtacgtgccc cacaacgtca
     6001 ccgacgtcca agcctgggat gccgacttcc tcgccttcag cagccacaag atgtgcggcc
     6061 cgagcggcat cggcgcgctg tggggccggg ccgaactgct cgacgcgatg cctccgttcc
     6121 tgggcggcgg caacatgatc gccgacgtcc gcctcgacgg gttcaccacc gcgccagtcc
     6181 cggccaagtt cgaagccggt actccggcga tcatcgaagc catcggcttc ggcgctgcgg
     6241 tcgactacct caccgggctg ggcatggcca acgtccgccg tcacgagatg gagatggcgg
     6301 cgtacgcgat cgactcgctc acccagcgct acggcgacga cgtcaccatc cacggccccc
     6361 gcaacgtcga agtgcgcggt gccacgctca gcttcgcgtt ccgcgacatc catccgcacg
     6421 acatcagcca ggtgctcgac cagcgcaacg tgtgcgtgcg cgccggccac cactgcgcca
     6481 agccgctgat gcgcctgctc ggggccaacg ccaccgtgcg ggccagcttc tacgtctaca
     6541 acgactctgc cgatgccgac tcgctcaccg atgccctcga gggtgcgagc gatatcttcg
     6601 gtctgtaagc acgccgccga tcggcatcga aaggcttgac catgcccggt ttggaagacc
     6661 tctaccgcga gatcatcctc gaccactacc gctcaccgcg caaccgtggc gagctcgatc
     6721 cgcctgccgt gcgcgtcgac gggcacaacc cgttgtgcgg cgacgagatc cagctctacc
     6781 tcgcggtcga cgacggcgtc gtcaccgaca tcaaagtcgg tgggtccggg tgcagcatca
     6841 gccagtcgtc ggccagcatg atgacccagg cggtcaaggg caagccgatc gccgaggtca
     6901 aggcgctggt ccggcgcttc aagggcatga tgagcgtcga cgaaatcgac gacgatcccg
     6961 ccgccgaagc cgccgcggcc gaggtcaagc tcggcgatct cgaggcgctg cagggcgtgg
     7021 tcaagttccc cgtgcgcatc aagtgcgcca ccttggcgtg gaacacgctg ctgcaggcga
     7081 tcgaccagac cacatcggcg tgatgccggc ctgacgccag cgtggtcccc gtctgacgcc
     7141 agcgtgatac ccggcgcgcg tgtcgccgcg ggaaattgcc atgatccggt gagaggattc
     7201 cgagcaccct cgactctgtc gggagagcga tcatcggctt cgctctcggg ccggggcgcg
     7261 tcgggcggcc accggtaggg tcggtcagat gaccaccgat tccgtacagg cagcccgcga
     7321 ccgagtcggc aaggcactgc gcgacttcgg ccatgcgttg gtcggccacc acatcgacgt
     7381 cgccggtctg gacgcgatca ccgccgacct cgacgcggcc cgcgcccaac tgctcgctac
     7441 cgaggaannn nnnnnnnccg atcgcgggac aacaccggct tccacgatcg ctggccagcc
     7501 atcgagcgcg acggcgaccc gctgcccacc tacggcgacc gaccgttctc cggtgtgagc
     7561 agcccgtgga gcgtcgagcc cgacgtcgtt cgcttcggtc ccggtgtgcg cgccaccgtc
     7621 acgttcggcg cggcccacga aggcgccccg ggtcgctgcc acggcggcat catcgccggg
     7681 ctgttcgacg acctgctcgg ctcagccgtg gcggtgctcg gcgtcgccgt gttcaccggc
     7741 gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngg
     7861 tcacccgcgc cacagccgtg ttcatccaac cccgcaccgc cgaatcccaa accaccttcg
     7921 accccacctg acgacgctac ggaagcagat cgagcatctc tggccgcgcc ttcatccccg
     7981 cctggatcac atcggcgggg gcgatcccgt gcttggttgc tgacggatgg accttcacgc
     8041 gagggcgagg gcgtcgcgga tcacctcgga gcgagacttc ccctcccgct gcgcccgagc
     8101 atcgagcgct gcgatctcgg cagtggtcag ccgcagcgcc accacctgcg acggttcggc
     8161 gccccgtcct ggacgcccct tccctcgctt cttcagcacc ccgatgtcgt agccgacctc
     8221 ggcttcgtcg ccccaggccg caatctgcgc gtcgatgact ggcactccgt tgatcgtctc
     8281 gcgttcagct acgtcgtgac cgttttacga aatacaccgt cacgaggctc cggcgtgtgg
     8341 gggcagaggc aagcggtggt cagcgtttga ggtcggtgag cgtgccgccg ctggccgcga
     8401 ccaggcggtc gggggcgatc gcgaacacgt cgtgccaggt g
//
LOCUS       DAORNF010000029         8432 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_6220_length_8432_cov_1.875731, whole genome shotgun sequence.
ACCESSION   DAORNF010000029 DAORNF010000000
VERSION     DAORNF010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 8432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8432
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6220_length_8432_cov_1.875731"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="PLV68_02115"
     CDS             complement(<1..383)
                     /locus_tag="PLV68_02115"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRE00062.1"
                     /translation="MTNERLSVPGEGGSVPHTDGVVAQGIVVGYDDSPTAAIAADWAA
                     LEAVRRGETLRLVACSVVPNSVDYYGVGTRQNAALELAAARLHQRHPGLAIEQTSTPH
                     DARDALAREAEGASMLVVGASGSHAL"
     gene            492..>985
                     /locus_tag="PLV68_02120"
     CDS             492..>985
                     /locus_tag="PLV68_02120"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRE00063.1"
                     /translation="MSTDDTRNAPGATGAGMTGTVTVGYDGSATSSAALRWAADQAAL
                     RGAALQIVSCYRIPVAAAGIEAWPAAESAVALLAVAEQQALAARDMALVEHPELTVTT
                     EVSAGPASAVLVDGAGPGDLIVVGASSHHGAAAFWLGSTPRRVIRHAPCPVAVVRSEA
                     SRGAA"
     assembly_gap    986..1172
                     /estimated_length=187
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1173..1389
                     /locus_tag="PLV68_02125"
     CDS             <1173..1389
                     /locus_tag="PLV68_02125"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRE00064.1"
                     /translation="GMTFKLGAKVTGVLQDASPQSALLGTVRDGDVLVVSTRGRGAVA
                     AGLFGSTVNAVLERAMVPVIVVPPPRS"
     assembly_gap    1498..1586
                     /estimated_length=89
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1587..2453
                     /locus_tag="PLV68_02130"
     CDS             <1587..2453
                     /locus_tag="PLV68_02130"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HRE00065.1"
                     /translation="RTPTTVIGGYIALTKPRIIELLLITTVPTMVLAEGGWPSTWLVV
                     VTLIGGTLAAGGANATNMYVDRDIDKLMVRTQGRPLVTGLIEPRNALIFACALEAAAF
                     AVLWAGANLLSAVLALSAALFYVFIYTLWLKRTSRQNIVIGGAAGAVPVLVGWAAVQN
                     NVTWAPVLLFIVMFLWTPPHFWALAIKHIDDYTAANVPMLPVVAPLPQVLRQMLWYTL
                     VMVAFTLVLAPVADLGAIYTIGAAVSGGLFILGTVALLRRPDPKAAMRLFGYSITYVT
                     LLFGVITLDVLI"
     gene            2671..>3456
                     /locus_tag="PLV68_02135"
     CDS             2671..>3456
                     /locus_tag="PLV68_02135"
                     /inference="COORDINATES: protein motif:HMM:NF012343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HRE00066.1"
                     /translation="MTTIDPTAHVESLTDAPARSAAGSTRLSALLGWLTTSDHKRLGR
                     MLFGSGLAALIAIAVVGALLGIDRADTGLLGSGTLAQLFAVHRIGLAYFAILPIVLGV
                     AVAIVPLQLGARSLAVPRLAAAGFWAWLIGSILVIVSLIANGGPLGGETKMVSLFIAS
                     YGVVLAGLFAIALALATTVLTTRAPGMNMRRIPLFSWSVLIASLGLVLVLPVALGTVV
                     YLWVSYRYNRQPFGGNAGLLGNGPQSSANWLGFTLTQPVTFLYA"
     assembly_gap    3457..3509
                     /estimated_length=53
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3510..>3805
                     /locus_tag="PLV68_02140"
     CDS             <3510..>3805
                     /locus_tag="PLV68_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00067.1"
                     /translation="GVVLAGMALVGTALFAGVTQTQPSLPWTGSKLYLGDMFWDKAQS
                     LVSYAFFVGLPLLGALIVLLVVPMAFQGVKPKISAALGFALFGFLLVLAGMVAN"
     assembly_gap    3806..3838
                     /estimated_length=33
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3839..>4226
                     /locus_tag="PLV68_02145"
     CDS             <3839..>4226
                     /locus_tag="PLV68_02145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00068.1"
                     /translation="AGTVYEEGVFVLIAYGVVLGMMGAIAYWGPKLWGRTMADKQVAP
                     LLALGLLAAVLASAPYLIAGFADQPADTATFSYGGPQNLWNLLSGIGHGLMVLTVLAF
                     VGLALRSFKVGPAAGDDPVDGLTLEWA"
     assembly_gap    4227..4246
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4328..4918
                     /locus_tag="PLV68_02150"
     CDS             4328..4918
                     /locus_tag="PLV68_02150"
                     /inference="COORDINATES: protein motif:HMM:NF012720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HRE00069.1"
                     /translation="MLALPPAPAPAPRRQLFIGASLIGAGGATLIGGMLATYMRFRDA
                     ARSGGGTWKPDAIAIPEVASNIMLFSFIAIGVFVQWAVYSAKRGDKKHTGMALGLNAV
                     IGLAIINAQAAVWAQIELPVNGAGDISGYGIMFYAVTGTFFVLLITGVVFTLITAFRY
                     LGGRTSETEIVTAHAIYWYFLTAAFCMLWYVVYVMK"
     gene            4934..6070
                     /locus_tag="PLV68_02155"
     CDS             4934..6070
                     /locus_tag="PLV68_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00070.1"
                     /translation="MFTTGSKLFLGATVLSLIATLVYGYGVGGKLGVLGLISVTVAFA
                     FLTGMNFWVRDSNVSAMDTAGIETCAAATAPPSNSMWPLVAGLGLALLPVGLVVGYAI
                     VWIAVILLLVATAEWMVLNWSDRASADAEYNTQVRRHIMHPFELPIAGAIGLGAVIFS
                     FSRIMLYKPGTTGMVLFGGIAAGVLLFGALIAAQRNVGPSLVAAVCSIGGVGLLAAGI
                     VTAIDGGHHIERHEIIGDAGTCAEEHTEADEKATRAIAAKANLAATITLKDGQLSAEV
                     LGIPGSPDPVTLLRGNDNFVKFRNLDGGDFRLVAYLGDEVANAGADNEVRTPDIVCTQ
                     AVSAGGTQFVVIKPPRPSWSAAPDEPFEFTVPGVESAVLAIEVP"
     gene            6172..7386
                     /gene="coxB"
                     /locus_tag="PLV68_02160"
     CDS             6172..7386
                     /gene="coxB"
                     /locus_tag="PLV68_02160"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HRE00071.1"
                     /translation="MTTTPRRWRSRLALPALVVGAITVLSACAKDAEQDTWQPAGSNA
                     RKIDNLQRPPFYIAGVVLVIVIAVIAFVVVRYRDRGQAIPQQTHGKPALEIGLTILPA
                     LILVAIGIPTVTTVLELDRTDDTECIINVTGQQWWWEYDYPVQEGCGGISAPIITSGQ
                     IVFPEDTNVLLRITSRDVIHSFWIPKLNGKKDAVPGRIHPLRMQADQPGIYAGQCTEF
                     CGLSHAYMRMEAVALTKDGFEQWKANQLEAYTSPVGDADALDGEAAFLANCSRCHQIN
                     GITDSTGNPVIARPDLYVYSQAAPNLTNLMTRNTFAGATYDLLTAECRDRVWNASPEA
                     FTDIYLEGVTEDCLDAVKLREWLRNAPAIKPMYTEADKLGPTDEKVRGMPNLNLSEDQ
                     IDDIIAFIKHLK"
     gene            7397..>8432
                     /locus_tag="PLV68_02165"
     CDS             7397..>8432
                     /locus_tag="PLV68_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HRE00072.1"
                     /translation="MALAERPAPLLRGATVTPSAALGVWRRPVATTGWRNWVFTVDHK
                     KLGLMYGAVSMFFFLVGGVEALLIRLQLATGDNKILSAGAYNQMFTMHATTMVFLFVM
                     PMTSAFANYLVPLQIGARDVAFPRINALGFWCYLAGGLFINTSWFLGGAADSGWFAYA
                     PNTSTEFSPSNGVDFWAMGLQITGIASLTGAINLIVTVLNMRAPGMKMMKVPVFTWMV
                     LVTQFLLLFAMPVITVALFLLTFQRNFGAAFFSVDKGADPLLWQHLFWIFGHPEVYIM
                     ILPSFGIISEVLPVFSKKPLFGYKFVVFSGAAIGFLGFGVWAHHMFTVGMSVDSQRYF
                     AFASMVIAVPT"
BASE COUNT         1191 a         2801 c         2574 g         1484 t
ORIGIN      
        1 agggcgtgcg agcccgacgc tccgacgacc agcatcgagg caccctcggc ttcgcgggcc
       61 agcgcgtcgc gcgcgtcgtg cggggtgctg gtctgttcga tcgccagccc tggatgacgt
      121 tggtgcagcc gggctgcggc caactcgagg gcagcgttct ggcgggtgcc gacgccgtag
      181 tagtcgaccg agttcggcac gacggagcag gcgaccaggc gcagggtctc gccccggcgg
      241 accgcttcga gcgcggccca atcggcggcg atggcagcgg tcggggaatc gtcgtagccg
      301 accacgatcc cctgggcaac gactccgtcg gtgtgcggca cgctgccgcc ctcgcctggc
      361 acggataggc gttcgttcgt catgactgca cgttgccacg acgacgtcgg ccgaggaggt
      421 gccatccgtc ggcccacccg gaggaccaat ggcccattgc cgctgcactg atcgtgcgag
      481 aagctgtgta catgagcacc gatgacactc gcaacgctcc gggcgcaacc ggagcaggta
      541 tgaccggcac cgtcacggtc ggttacgacg gcagcgcaac gtcgtcggcc gccttgcgtt
      601 gggcggccga tcaggccgcg ctgcgtggtg cggcgctgca gatcgtgtcg tgctaccgca
      661 tccccgtcgc tgccgcgggg atcgaggcgt ggcctgccgc cgagtcggcg gtcgcgctgt
      721 tggctgttgc cgaacagcaa gccctcgcgg cgcgggacat ggccttggtc gagcatcccg
      781 agctgaccgt gaccaccgag gtgtcggccg gtccggcttc ggcggtactc gtcgacgggg
      841 ccggtcccgg cgatctgatc gtggtcggcg ccagcagtca ccacggagcg gcagcgttct
      901 ggttgggcag cacgccgcga cgtgtcatcc gtcatgcgcc ctgcccggtc gcggtcgtgc
      961 gcagcgaggc cagtcgtggt gctgcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngggcatga ccttcaaact gggcgccaag
     1201 gtcaccggcg tgctgcaaga cgcgtcgccg cagtcggcgc tgctcggtac cgtgcgcgac
     1261 ggcgacgtcc tggtggtcag cacgcgtggt cgcggtgcag tcgccgccgg tctgttcggc
     1321 tcgaccgtca acgccgtgct cgaacgggcg atggtgccgg tgatcgtcgt ccccccgcca
     1381 cgcagctgat gaggcccgca gctgatttga accgcagctg atttgaaccg ccgctgactt
     1441 ggcccgcaac cgatcggtga gccgttcggg gacatgccga ttcggaagcg tcgaggcnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnncgta cgcctacaac cgtgattggc gggtacatcg
     1621 cgctcaccaa gccgcggatc atcgaactgc tcctgatcac cacggtgccc acgatggtgc
     1681 tcgccgaagg cggctggccc tcgacgtggc tggtggtggt aactctgatc ggcggcaccc
     1741 tcgccgccgg tggggccaac gccaccaaca tgtacgtcga ccgtgacatc gacaagctga
     1801 tggtgcgcac ccagggccgc cccttggtca ccgggctgat cgaaccgcgc aacgccctga
     1861 tcttcgcctg cgcactcgaa gcggctgcgt tcgccgtgct gtgggctggg gccaacctgt
     1921 tgtcggcggt gctggccctg tcggcagcgc tgttctacgt gttcatctac acgctgtggc
     1981 tgaagcgcac gagtcgccag aacatcgtca tcggcggtgc cgccggggcg gtgccggtgc
     2041 tggtcggctg ggccgcggtg cagaacaacg tcacctgggc accggtgctg ctgttcatcg
     2101 tgatgttctt gtggaccccg ccgcacttct gggcgctggc catcaagcac atcgacgact
     2161 acaccgcggc aaacgtgccg atgctgcccg tggtggcgcc gctgccccag gtgttgcgcc
     2221 agatgttgtg gtacacgctg gtcatggtcg cgttcaccct ggtgctggca ccggtggccg
     2281 atctgggggc catctacacc atcggtgctg cggtgtcggg tggcttgttc atcctcggca
     2341 cggtggcgct gctgcgccgc cccgacccca aagcggcgat gcgcctgttc ggctacagca
     2401 tcacctacgt caccctgctc ttcggggtga tcacccttga tgtgctgatc tgaacggcga
     2461 cgtgctgatc tgacgcctgc atccgaattc ggacacttgg cgcctgtgtg ggtattgtcg
     2521 agggctgaac ggaaccgaat ccgcgctttc cttgttggtg gcccgcgcat ccggtgctgt
     2581 ccaatcgacg actgaaccca ccatgcatga ccggacgacg agagaactga tgctgacggc
     2641 accgttgacg acagaactga gcctttgccc atgacgacga tcgaccccac cgcccacgtc
     2701 gagtcgctca ccgacgcacc ggcacgctcc gcggctggtt cgacacgcct cagcgcactg
     2761 ttggggtggc tcaccaccag cgatcacaag cgtctcggcc ggatgttgtt cggcagcggc
     2821 ctggcggcgc tgatcgcgat cgcggtcgtc ggcgccctgc tcggcatcga ccgggccgac
     2881 accggcctgc tcggcagcgg cacgctggcc caactgttcg cggtgcaccg catcgggctg
     2941 gcctacttcg ccatcctccc gatcgtgctc ggcgtcgccg tggcgatcgt gccgctgcaa
     3001 ctcggcgcgc ggtcgctggc cgtgccccgc ctcgctgccg cgggcttctg ggcttggctg
     3061 atcggttcga tcctggtcat cgtcagcctc atcgccaacg gcgggccgct tggcggcgag
     3121 accaagatgg tgtcgctgtt catcgcctcc tacggcgtgg tgctggccgg tctgttcgcg
     3181 atcgccctgg ccctggccac cacagtgctc accacccgtg cgccgggcat gaacatgcgc
     3241 cgcatcccgc tgttctcctg gtcggtgctg atcgcctcgc tcggtctggt gctcgtgttg
     3301 ccggtggcgc tcggcacggt ggtgtacctg tgggtcagct accgctacaa ccgccagccc
     3361 ttcggcggta acgccgggct gttgggtaac ggtccccagt ccagcgccaa ctggctcggc
     3421 ttcacgctga cccagccggt caccttcctg tacgccnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc ggcgtggtgc tggcgggcat ggccctggtc
     3541 ggtaccgccc tgttcgccgg ggtcacccag acccagccga gcctgccctg gaccggctcc
     3601 aagctgtacc tcggcgacat gttctgggac aaggcccaga gcctggtcag ctatgcgttc
     3661 ttcgtcggct tgccgctgct cggcgcactg atcgtgctgc tcgtggtgcc gatggccttc
     3721 cagggcgtca agccgaagat ctcggctgcg ctcgggttcg ccctgttcgg cttcctgctg
     3781 gtgttggccg gcatggtggc caacgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngg
     3841 ccggcacggt gtacgaggag ggtgtgttcg tcctcatcgc ctacggcgtc gtgctcggca
     3901 tgatgggcgc gatcgcctac tggggcccca agctgtgggg ccgcaccatg gccgacaagc
     3961 aggtcgcccc gttgctcgcc ctcgggctgt tggccgcggt gctggcgtcg gcgccgtacc
     4021 tgatcgccgg ttttgccgac cagcccgccg acaccgccac gttcagctac ggcggcccgc
     4081 agaacctgtg gaacctgctc agcggcatcg gccacggctt gatggtgctc acggtgttgg
     4141 ccttcgtcgg cctcgcgttg cgcagcttca aggtcggccc cgctgcgggt gacgatccgg
     4201 tcgacggcct gaccctggaa tgggccnnnn nnnnnnnnnn nnnnnncaac ttcgtcgccg
     4261 tccacaccgt tgcctcggcc cagccgctgg ccgatctcaa gcctgcgacc ggatcggaga
     4321 actcctgatg ctcgcgttgc cccccgcccc ggcccccgcg ccgcggcgtc aactgttcat
     4381 cggcgcgagc ttgatcggcg cgggtggtgc caccctgatc ggcggcatgc tcgcgaccta
     4441 catgcggttc cgcgacgcgg cccgctcggg cggtggcacg tggaagcccg acgccatcgc
     4501 gatccccgag gtggcgtcga acatcatgct gttcagcttc atcgccatcg gggtgttcgt
     4561 gcagtgggcg gtctattcgg ccaagcgcgg cgacaagaag cacaccggca tggccctcgg
     4621 cctcaacgcc gtcatcggcc tggcgatcat caacgcccag gccgcggtgt gggcccagat
     4681 cgaactgccg gtcaacggcg ccggcgacat ctccggctac gggatcatgt tctacgcggt
     4741 caccggcacg ttcttcgtcc tgctgatcac cggcgtggtg ttcacgctga tcaccgcctt
     4801 ccgctacctc ggtggacgca cctccgagac ggagatcgtc accgcccatg cgatctattg
     4861 gtatttcctc accgcagcgt tctgcatgct gtggtacgtc gtctacgtca tgaagtgagc
     4921 aggtaacccg cacatgttca ccaccggttc caaactcttc ctcggcgcca cggtcctgtc
     4981 gctgatcgcc accctcgtct acgggtacgg cgtcggcggc aagctgggcg tgctcggcct
     5041 gatctcggtc accgtcgcgt tcgcattcct cacgggcatg aacttctggg ttcgtgacag
     5101 caacgtgtcg gcgatggaca ccgccggcat cgagacctgt gccgccgcga ccgctccgcc
     5161 gtccaacagc atgtggccgc tcgtcgccgg tctcggtctg gcgctgctcc cggtcggcct
     5221 cgtcgtcggc tacgcgatcg tgtggatcgc cgtcatcctg ctgctcgtgg ccaccgccga
     5281 gtggatggtc ctcaactgga gcgaccgggc ctcggccgac gccgagtaca acacccaggt
     5341 ccgccgccac atcatgcacc cgttcgagct gcccattgcc ggtgcgatcg ggctcggcgc
     5401 ggtcatcttc tcgttctcac ggatcatgct ctacaagccg ggcaccaccg gcatggtgct
     5461 gttcggtggc atcgctgccg gcgtgctgct gttcggtgcg ctgatcgccg cgcaacgcaa
     5521 cgtcggcccc tcgctggtcg cggccgtgtg cagcatcggc ggcgtgggcc tgttggccgc
     5581 tggcatcgtc accgcgatcg acggtggtca ccacatcgag cggcacgaga tcatcggcga
     5641 cgcgggtacg tgcgcggaag agcacaccga ggctgacgaa aaggcaaccc gggccatcgc
     5701 ggccaaggcc aacctggccg ccacgatcac cctcaaggac ggccagctct ctgccgaagt
     5761 gctcggcatc cctggcagcc ccgatcccgt caccttgctg cgtggcaacg acaacttcgt
     5821 caagttccgc aacctcgacg gcggcgactt ccgcctggtg gcctacctcg gcgacgaagt
     5881 ggccaacgcc ggggccgaca acgaggtccg caccccggac atcgtctgca cccaagccgt
     5941 gtccgcaggg ggcacgcagt tcgtcgtcat caagccgccg cgcccgtcgt ggtcggccgc
     6001 cccagacgag ccgttcgagt tcaccgtgcc cggcgtggag tcggcggtgt tggcgatcga
     6061 ggtcccgtga gccgccccga tccgatgtgc cgcgagaccg cccagcggat gggggaccat
     6121 ctccgcggca cgactaccgt tgaccgagtg cccacacaat cgggagatcc gatgacaacc
     6181 acacctcgcc gttggcgttc ccgcctggct ttgccagcgc tggtcgtcgg cgcgatcacc
     6241 gtgctgtccg cctgtgccaa ggatgccgag caagacacct ggcagcccgc aggcagcaac
     6301 gcgcgcaaga tcgacaacct ccaacgtccg ccgttctaca tcgccggcgt ggtgttggtc
     6361 atcgtcatcg ccgtcatcgc cttcgtggtc gtccgctacc gcgatcgcgg ccaggcgatt
     6421 ccccaacaga cccacggcaa gcccgcgctc gagatcggcc tcaccatctt gcccgcgctc
     6481 atcctcgtgg cgatcggcat ccccacggtc accaccgtgc tcgagctcga ccgcaccgac
     6541 gacaccgagt gcatcatcaa cgtcaccggc cagcagtggt ggtgggagta cgactacccg
     6601 gtgcaggagg gttgcggtgg catcagcgcg cccatcatca ccagcggtca gatcgtgttc
     6661 cccgaagaca ccaacgtgct gttgcgcatc accagccgtg acgtcatcca ctcgttctgg
     6721 atccccaagc tcaacggcaa gaaggacgcg gtccccggtc gcatccaccc gctgcgcatg
     6781 caggccgacc agcccggcat ctacgccggc cagtgcaccg agttctgcgg tctctcccac
     6841 gcctacatgc gcatggaggc ggtggccctc accaaggacg gcttcgagca gtggaaggcc
     6901 aaccagctcg aggcctacac ctcgcccgtg ggagacgccg acgccctcga cggtgaagcc
     6961 gcgttcttgg ccaactgcag ccgctgccat cagatcaacg gcatcaccga ctcgaccggc
     7021 aacccggtga tcgcccgccc tgacctgtac gtgtactcgc aggctgcgcc gaacctcacc
     7081 aacctgatga cccgcaacac cttcgccggt gccacctacg acctgctcac ggccgagtgc
     7141 cgtgaccggg tgtggaacgc ctcacccgag gcgttcaccg acatctacct cgaaggcgtc
     7201 accgaagact gcctcgacgc ggtcaagctg cgtgagtggc tgcgcaacgc gccggctatc
     7261 aagcccatgt acaccgaggc cgacaagctc gggcccaccg acgagaaggt gcgtggtatg
     7321 ccgaacctga acctcagcga ggatcagatc gacgacatca tcgctttcat caagcacctg
     7381 aagtaaggag acacggatgg ctctcgcaga acgtcctgcc cccctgttgc ggggagcgac
     7441 cgtcacgccg tccgccgcgc tcggcgtgtg gcggcgcccg gtggctacca ccgggtggcg
     7501 caactgggtc ttcacggtcg accacaaaaa gctcggcctc atgtatggcg ccgtgtcgat
     7561 gttcttcttc ctcgtcggcg gcgtcgaagc gctgctgatc cggttgcagc tcgccacggg
     7621 ggacaacaag atcctcagcg ccggtgccta caaccagatg ttcaccatgc acgccaccac
     7681 catggtgttc ttgttcgtga tgccgatgac ctcggccttc gccaactacc tcgtgccgct
     7741 gcagatcggc gcccgtgacg tcgccttccc ccgcatcaac gctctcgggt tctggtgcta
     7801 cctggccggc ggtctgttca tcaacacctc gtggttcctc ggtggcgcgg ccgacagcgg
     7861 ctggttcgcc tacgctccca acaccagcac cgagttctca ccgagcaacg gtgtcgactt
     7921 ctgggcgatg ggcttgcaga tcacgggtat cgccagcttg accggcgcga tcaacctgat
     7981 cgtcacggtg ctcaacatgc gagcaccggg gatgaagatg atgaaggtac ccgtgttcac
     8041 ctggatggtg ttggtgacgc agttcttgct gttgttcgcc atgccggtca tcacggtggc
     8101 gttgttcctg ttgacgttcc agcgcaactt cggggccgcg ttcttcagcg tcgacaaggg
     8161 tgccgatccg ttgctgtggc agcacctgtt ctggatcttc ggtcaccctg aggtgtacat
     8221 catgatcttg ccgtccttcg gcatcatcag cgaggtactc ccggtcttct ccaagaagcc
     8281 gctgttcggt tacaagttcg tggtcttctc gggggccgcg atcggcttcc tcggcttcgg
     8341 cgtgtgggct caccacatgt tcaccgtcgg gatgtcggtc gattcgcaac gctatttcgc
     8401 cttcgcctcg atggtgatcg ccgtgcccac cg
//
LOCUS       DAORNF010000030         8361 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_6293_length_8361_cov_1.865037, whole genome shotgun sequence.
ACCESSION   DAORNF010000030 DAORNF010000000
VERSION     DAORNF010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 8361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8361
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6293_length_8361_cov_1.865037"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..451
                     /locus_tag="PLV68_02170"
     CDS             <1..451
                     /locus_tag="PLV68_02170"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HRE00073.1"
                     /translation="AEVEFFLRLHGLWEGVVALPPPPEPPFDIETMEPKIAEVAKMIE
                     WSARTTAADVVAAGDACTALNHQLLAAFEHCDLLLTPTLAFDPEPIGQPDRRWIKATY
                     PFNLTRSPAGTVPIGTSPVGLPIGLQIVGPQHADTPVLALMHHLTGA"
     gene            complement(470..1474)
                     /locus_tag="PLV68_02175"
     CDS             complement(470..1474)
                     /locus_tag="PLV68_02175"
                     /inference="COORDINATES: protein motif:HMM:NF016274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HRE00074.1"
                     /translation="MPAEFAPHERTLIAWPARHSLYGDLMGAAEDAHAQVARAIADYE
                     LVTMIVDPGPAVARAAELCGPRVDIVELPIDDSWFRDSGPIYVVDGTDRDGGARVALD
                     FQFNGWGQKYAPWDRDDLVASRWAAHAGHASRRIGMVLEGGSICVDGEGTLLTTTQCL
                     MHPNRNPGLTRTEIEQRLCAELGVSMVLWLPYGLADDDDTDGHVDNVAAFARPGSVVV
                     QGCDDPAEPDWLRMDVNARWLAGSLDAAGRPIEVVSIPVLPFAEVGGRRVPVPYVNYY
                     LGNGFVLVPTCGHPADADMLAIIAAQYPGRETVSLDVGAILAYGGGGIHCITQQLPAP
                     "
     gene            complement(1483..>1750)
                     /locus_tag="PLV68_02180"
     CDS             complement(1483..>1750)
                     /locus_tag="PLV68_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00075.1"
                     /translation="GVGGEREITFYGSSFIADSTGAKVAEAGRDGEAVLTASFDLAAL
                     GTMRHAWGLFRDRRPDLYGPLLTLDGGSRDGGFDGQPRNGGRST"
     assembly_gap    1751..1791
                     /estimated_length=41
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1792..2387)
                     /locus_tag="PLV68_02185"
     CDS             complement(<1792..2387)
                     /locus_tag="PLV68_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012537242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HRE00076.1"
                     /translation="MNVTVAAVQMGMGEAVADNIATAERLVREAAGRGAQIVLIPELF
                     EGPYFCIDMLPKHFARAHPVQGHPTVEHFRAVAAELGVVLPLSIYERANNATFNTVVI
                     VDADGTVLGTYRKSHIPDGPGYTEKYYFNPGDTGFRVWRTRYATIGVAICWDQWFPES
                     ARVMALQGAELLFYPTAIGSEPPDPAWDGVRDRLQRAAA"
     gene            complement(2413..>3529)
                     /locus_tag="PLV68_02190"
     CDS             complement(2413..>3529)
                     /locus_tag="PLV68_02190"
                     /inference="COORDINATES: protein motif:HMM:NF015043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HRE00077.1"
                     /translation="RELSEFRGQPDIPGPNWNGSGGGPNTNVGGFAGTTWAMVEAGGC
                     PVELTYELETVGRNDFFGTLPGAFTAHPKVDPDTREMHAMVYALPQWLDHVQYVVVAP
                     DGTVRRTVDVPLGQTTMLHDMSLTGRYAVVYDFPCRVDIQMVLDGFNFPLRWQPGTGC
                     RVGLLPREAEADSIVWIDLPDCYSFHPMNAYDDADGSVVIDLVVYDSMFDRERTGPFS
                     EQMGRLERWVLNPATRTANITVIDERSQEFPRLRGSLTAKPYRYGYCAAVDQSAIEWP
                     TVKHDLHTGERTEFDHGTGRAAGEPVFIPREGSTAEDDGWLMTLVHDVPNATAELAVW
                     DAQDFARGYVAQVQLPQRVPFGFHGNWVSDRSVPEPT"
     assembly_gap    3530..3583
                     /estimated_length=54
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3584..3793)
                     /locus_tag="PLV68_02195"
     CDS             complement(<3584..3793)
                     /locus_tag="PLV68_02195"
                     /inference="COORDINATES: protein motif:HMM:NF015043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016702 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of two atoms of oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HRE00078.1"
                     /translation="MSTDTTSHTSTNLYLTGNYAPVRDEVTAFDLPIVGQLPAELNGR
                     YLRNGPNPIRPVGANYHWFTGDGMVH"
     gene            3903..4532
                     /locus_tag="PLV68_02200"
     CDS             3903..4532
                     /locus_tag="PLV68_02200"
                     /inference="COORDINATES: protein motif:HMM:NF024701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRE00079.1"
                     /translation="MADDLSTPQSGGPPQVTPNPAREALLAAALDIIDREGSSALTVR
                     SVAKAAGCSTTGVYTWFGGKNGLVEQIFVDSFRQFGAHLLAAKGVRSTTGDRALRALG
                     HAYREWALANPTRYMVMFGKAVPDYEPSMEARTVGLTSFAQLTDVTAAHLRRVGSTLD
                     PLDIAFHLWAGVHGYVSLEMAGVDLAANDRERAARFEVGIHRLLRGCLP"
     gene            complement(4546..6249)
                     /locus_tag="PLV68_02205"
     CDS             complement(4546..6249)
                     /locus_tag="PLV68_02205"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RyR domain-containing protein"
                     /protein_id="HRE00080.1"
                     /translation="MRSSRGRWYLLASLWVLLIVLGFGGFRQQARDSGEPQSSLETLY
                     LLVQLAALEFDASSDFLNWRLQVARFVAPAMAATTVLQTASLVFVDQFRRFRLRFVRN
                     HTVVCGLGEIGTRLAIAFADAGERVVAIASERDADGAGTVGDHDVTVLTGDQTDRALL
                     AAARVQTASRLVAVTGDDATNVSIALHAVDVVGDRRTNALRCAVHLADTELTSLLRAA
                     DLDAHRGMRISFFTTHERGARAWLAEHPPLLDDDDPVHVAVFSLGQLGRSLVVNIAQQ
                     WLQYRPGERLRITMVDPSASGRWQALTLQHPALAQCCEATVIDFDFDKPTAASVAALQ
                     ELFADDLAPTWIAYVDESTSLSTAFFITRSLGVDRTPLVVRTRTAGGLGALLIPGTTA
                     NDPFPSMHAFPFLDRTCTPESVDGGAREQLAEAVHEDYVRQAGGRSAAADDALLRPWS
                     ELTEAQRELSRRRVDGIIADLAAVGHVLAPLRRWEDVAQPLSDAQIETLAERDHQRWF
                     DDRVAAGWSYGEVRDNALKHNPLLVAWRELPHDAQAANRATAAGLGAMLARAGFEALP
                     R"
     gene            complement(6255..>6464)
                     /locus_tag="PLV68_02210"
     CDS             complement(6255..>6464)
                     /locus_tag="PLV68_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00081.1"
                     /translation="IVPSDPADPTRQRAYETAGREMVATSDVVIALWDGAPARGRGGT
                     AEMIDQALELGVAVEVVLVNREATS"
     assembly_gap    6465..6604
                     /estimated_length=140
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6605..6819)
                     /locus_tag="PLV68_02215"
     CDS             complement(<6605..6819)
                     /locus_tag="PLV68_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00082.1"
                     /translation="MSDIGIGDAGIDDFGKTPSALCIGVTGHRTYLDPDQVQACVDAV
                     LDRLWPALDAADAASVVVVSSLAEGADR"
     gene            complement(6819..>8361)
                     /locus_tag="PLV68_02220"
     CDS             complement(6819..>8361)
                     /locus_tag="PLV68_02220"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HRE00083.1"
                     /translation="GRDGTLAVRYQLDLDATPRTFRLRVPPGARFAAVDGKPIALDGA
                     RYADLTVDGSVTITYELSGHVQRFADAAVVTLGGPTGSNRTLDSDLSLFGCPRCSLSD
                     AGYGEVPVTIALYTEAPSTIADTGTVWLSNTLDRRHVRTHHDASTDTGAASGPLVLQA
                     VTRGDAVALIATFPADAVPDVARSSGASAVEIDAVIESIDDGARSFTAVGRDGGGPSV
                     FWSVVMTVLALIAVVLVAHGLVSHIPFGGIRRRGQPDGVGGEVPRPSTAERPDDLTPV
                     AVALVTEPARNRVQRATAAALLSMGERGAISIAGTERRLTLFVPDSAELQGPFERAVL
                     DQLVVYGTRASQSVAGERRYVGPRLWGSAGVTATASLRSGLQMEGANLGLIRRRPLAV
                     AMIPLAIAFAVIAIVVFDGIGVLAVLTAVAGIIGAIAISLMTPPMPTARGRAHAAVWN
                     QYAGWHQPDDVQRASVSAIWTMGSDLTYGAALGTARKVVEALGPPPPAGRRGAKQPRG
                     SKVAS"
BASE COUNT         1281 a         2871 c         2765 g         1209 t
ORIGIN      
        1 ggccgaagtc gagttcttcc tgcggctgca tggtctgtgg gagggagtcg tcgccctgcc
       61 gcccccgccc gaaccgccct tcgacatcga gacgatggag ccgaagatcg ccgaggtcgc
      121 gaagatgatc gagtggtcgg cccgcaccac cgccgccgac gtcgtcgccg ccggggacgc
      181 gtgcaccgcg ctcaaccacc agttgctcgc cgcgttcgag cactgcgacc tgctgctcac
      241 gccgacgttg gcgttcgacc ccgaaccgat cggccaaccc gatcggcgct ggatcaaggc
      301 gacctacccg ttcaacctca cccgcagccc ggcaggcacg gtaccgatcg gcaccagccc
      361 ggtcggcctc cccatcggcc tccagatcgt cggcccccaa cacgccgaca cccccgtgct
      421 cgccctgatg caccacctca ccggtgccta gcccgacgcc cgctgctcgt cagggggcgg
      481 ggagttgttg ggtgatgcag tggatgccgc ctcctccgta ggcgaggatc gcgccgacgt
      541 cgagggacac ggtctcgcgg ccggggtatt gggcggcgat gatcgccagc atgtccgcgt
      601 cggcggggtg accacaggtg ggcaccagca cgaagccgtt gccgaggtag tagttgacgt
      661 acggcacggg cacccggcgc ccgccgactt cggcgaaggg cagcaccggg atcgacacga
      721 cctcgatcgg ccgtccagcg gcatcgagcg atccggccag ccagcgggcg ttgacgtcca
      781 tccgtagcca atcgggctcg gcggggtcgt cacagccctg gaccacgacg gagcccggcc
      841 gggcgaacgc cgccacgttg tcgacgtggc cgtcggtgtc atcgtcgtcg gccagcccgt
      901 acggcagcca cagcaccatc gacacgccga gttcggcgca cagccgttgc tcgatctcgg
      961 tgcgggtcaa gcccgggttg cggttggggt gcatcaggca ctgcgtcgtg gtcagcagcg
     1021 tgccctcgcc gtcgacgcag atcgagccgc cttccaacac catgccgatg cggcgcgacg
     1081 cgtggccagc gtgggccgcc caccgcgacg cgaccagatc gtcccgatcc cacggtgcgt
     1141 atttctggcc ccacccgttg aactggaagt cgagcgcgac ccgcgcgccg ccgtcacgat
     1201 ccgtcccatc aacgacgtag atcgggccgc tgtcgcgaaa ccacgagtcg tcgatcggta
     1261 gctcgacgat gtcgacgcgc ggcccacaca actccgccgc ccgtgccact gccgggcctg
     1321 gatcgacgat catcgtcacc agctcgtagt ccgcgatcgc gcgggcgacc tgggcgtggg
     1381 cgtcctcggc cgcgcccatc agatcgccgt acaacgagtg gcgcgcgggc caggcgatca
     1441 gcgtccgttc atgcggcgcg aactcggcgg gcatgcgcgc gttcacgtgc tgcgtccgcc
     1501 gtttcgcggt tggccgtcaa acccgccgtc gcgcgaccct ccgtcgagcg tcagcagcgg
     1561 gccgtacaga tcggggcggc ggtcacggaa cagaccccag gcgtggcgca tcgtgcccag
     1621 cgcggccagg tcgaacgagg cggtcagtac cgcctcaccg tcacgcccgg cctctgccac
     1681 cttggccccg gtggagtcgg cgatgaacga actgccgtag aacgtgatct cccgctcgcc
     1741 acccaccccg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngccgcagcg
     1801 cgttgcaggc ggtccctgac accgtcccag gccgggtcgg gcggctcgct gccgattgcc
     1861 gtcgggtaga acagcaactc ggcaccttgc agcgccatga cccgggccga ctcgggaaac
     1921 cactgatccc aacagatcgc cacaccgatc gtggcgtacc gcgtgcgcca cacccgaaag
     1981 ccggtgtcgc cggggttgaa gtagtacttc tcggtgtagc ccggcccgtc ggggatgtgg
     2041 ctcttgcggt acgtgcccag cacggtgccg tcggcatcga cgatcaccac cgtgttgaac
     2101 gtggcgttgt tggcccgctc gtagatgctc agcggcagca ccacgcccaa ctcggcggcg
     2161 accgcgcgga agtgctcgac ggtcgggtgg ccctgcaccg gatgggcgcg ggcgaagtgc
     2221 ttgggcagca tgtcgatgca gaagtacggc ccttcgaaca gctcggggat cagcacgatc
     2281 tgcgcaccgc gcccggccgc ctcacgcacc aaccgctccg cggtggcgat gttgtccgcc
     2341 accgcctccc ccatccccat ctgcaccgcc gccaccgtga cgttcatcgc ccctgtctag
     2401 cggaattgcg ggtcaggtcg gttcggggac ggagcggtcg ctgacccagt tgccgtggaa
     2461 gccgaaggga acgcgctggg gcaactgcac ctgggcgacg tagccgcggg cgaagtcctg
     2521 ggcgtcccac accgccaact cggcggtcgc gttgggaacg tcgtgcacga gcgtcatcaa
     2581 ccatccgtcg tcctcggcgg tcgatccttc gcgcgggatg aacaccggct cgcccgcggc
     2641 gcgaccggtc ccgtggtcga actccgtccg ctcaccggtg tgcaggtcgt gcttgacggt
     2701 cggccactcg atcgcgctct ggtcgacggc cgcgcagtac ccgtagcggt acggcttggc
     2761 ggtgaggctg ccgcggaggc gggggaactc ttgcgagcgc tcgtcgatca cggtgatgtt
     2821 ggccgtgcgc gtcgccgggt tgagcaccca ccgttcgagc cggcccatct gctcggagaa
     2881 ggggccggtc cgctcgcggt cgaacatcga gtcgtagacc accaggtcga tcaccaccga
     2941 gccgtcggcg tcgtcgtagg cgttcatcgg gtggaacgag tagcagtcgg gcaggtcgat
     3001 ccacacgatc gagtccgctt cggcctcacg cggcaacaag ccgacccgac acccggtacc
     3061 gggctgccag cgcaacggga agttgaagcc gtcgaggacc atctgaatgt cgacccggca
     3121 cgggaagtcg tacaccaccg cgtagcgccc ggtgagcgac atgtcgtgca gcatcgtggt
     3181 ctggccgagc ggcacgtcga ccgtgcgccg caccgtgccg tcgggcgcga ccaccacgta
     3241 ttgcacgtga tcgagccact gcggcagggc gtagaccatc gcgtgcatct cgcgggtgtc
     3301 ggggtcgacc ttcggatggg ccgtgaacgc accgggcagg gtgccgaaga agtcgttgcg
     3361 cccgacggtc tccagttcgt aggtcaactc gaccgggcag ccgccagctt cgaccatcgc
     3421 ccacgtcgta ccggcgaaac cgccgacgtt ggtattcggc ccaccgccgc tgccgttcca
     3481 gttggggccg ggaatgtccg gctggccacg gaactcgctg agctcgcggn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngtgcacc atgccgtcgc
     3601 cggtgaacca gtggtagttc gctcccaccg gccggatcgg gttcggcccg ttgcgcaggt
     3661 agcgcccgtt caactcggcc ggcagttggc cgacgatcgg caagtcgaag gcggtcacct
     3721 cgtcccgcac cggcgcgtag ttcccggtga gatagaggtt ggtgctcgtg tgggacgtcg
     3781 tgtcggtgct catgggcgca cctctccttt cggttgtgca cggatctcga cgggtttcat
     3841 ggggtgacga cgggtgcgtg ctgtaacagt gttacgtcga gccgtgggtg taacattgtt
     3901 acgtggccga cgatctgtca accccccaat ccggcggacc gccacaggtc acacccaacc
     3961 cggcccgcga agcgctgctc gccgcggcac tcgacatcat cgaccgcgag ggatcgagcg
     4021 cgctcaccgt ccgctcggtg gccaaggcgg cgggctgttc gaccaccggt gtgtacacct
     4081 ggttcggcgg caagaacggc ttggtcgagc agatcttcgt cgacagcttt cgccagttcg
     4141 gcgcccactt gctcgccgcc aaaggcgtgc gcagcacgac cggcgaccgc gcgctgcgcg
     4201 ccctcggcca cgcctaccgg gagtgggcgc tggccaaccc aacccgctac atggtgatgt
     4261 tcggcaaggc cgtacccgac tacgaaccaa gcatggaggc acgcaccgtc gggctgacgt
     4321 cgttcgccca actcaccgac gtgaccgccg cccacctgcg ccgagtgggc tcgacgctcg
     4381 acccactcga catcgccttt cacctctggg ccggagtgca cggctacgtg tcgctggaga
     4441 tggccggggt cgacctcgcc gccaacgacc gcgagcgcgc cgcccgcttc gaagtcggca
     4501 tccaccgcct cctccgcggc tgtctcccct gacggcgcgg acgcctcagc gggggagggc
     4561 ttcgaagccg gctctggcga gcatggcgcc gaggccggca gccgtcgcgc ggttcgcggc
     4621 ttgtgcgtcg tgtggtagct cacgccaggc gaccaacaac gggttgtgct tcagcgcgtt
     4681 gtcgcgcacc tcgccgtagc tccacccggc cgcgacgcgg tcgtcaaacc agcgctggtg
     4741 atcacgctcg gcgagcgtct cgatctgcgc gtcgctgagg ggttgggcga cgtcctccca
     4801 acgccgcaac ggcgcgagca cgtggccgac cgcggcgaga tcggcgatga tgccgtcgac
     4861 gcggcggcga ctgagctcgc gctgcgcttc cgtgagctcg ctccatggac gcagcagcgc
     4921 gtcgtccgcg gcagccgatc gtcccccggc ctggcggacg tagtcctcgt gcaccgcttc
     4981 ggcaagttgc tctctggcac cgccgtcgac cgactcgggc gtacacgtcc ggtcgagaaa
     5041 cggaaatgca tgcatcgacg ggaacggatc gttggccgtg gtgccaggga tcaacagcgc
     5101 gcccaatccg cccgcggtgc gggtgcggac gacgagcggt gtgcggtcga caccgaggct
     5161 gcgggtgatg aagaacgcgg tcgacagcga cgtcgactcg tcgacgtacg cgatccacgt
     5221 cggcgccagg tcgtcggcaa acagctcctg cagcgcggcg acactggccg cggtcggctt
     5281 gtcgaagtcg aagtcgatca ccgtcgcctc gcagcactgc gccaacgcgg ggtgttgcag
     5341 cgtcaacgcc tgccaccgtc cactggccga cgggtcgacc atcgtgatcc gcagccgctc
     5401 gccgggacgg tactggagcc actgctgggc gatgttgacc accaggctgc gacccagttg
     5461 accaaggctg aacacggcga catgaaccgg atcgtcatca tcgagcagcg gtgggtgttc
     5521 ggccaaccag gcccgcgcgc cgcgctcgtg ggtggtgaag aagctgatcc gcatgccgcg
     5581 atgggcatcg agatcggcgg cgcgcagtaa cgacgtcagt tcggtgtcag ccaggtgcac
     5641 cgcacaccgc aacgcattgg tgcggcggtc gcccacgacg tcgacggcgt gcagcgcgat
     5701 cgacacgttg gtcgcatcgt caccggtgac agcgacgaga cgggaagcgg tctgcacccg
     5761 cgccgcggcc agcagggcgc gatcggtctg gtcgccggtc agcaccgtga cgtcgtggtc
     5821 gccgacagtc cccgctccgt cggcgtcgcg ctcggacgcg atcgcgacga cccgctctcc
     5881 ggcgtcggcg aacgcgatcg ccagccgcgt cccgatctcg ccgagaccgc acaccaccgt
     5941 gtgattgcgt acgaaccgca accgaaagcg gcggaactga tcgacgaaca ccaacgaggc
     6001 cgtctgcaac acggtggtcg ccgccatcgc cggagcgacg aagcgggcga cctgcagccg
     6061 ccagttcagg aagtcgctgc tggcgtcgaa ctcgagcgcc gccaactgca ccagcaggta
     6121 cagggtctcc aggctcgact gcggctcgcc gctgtcgcga gcctgctggc ggaaaccgcc
     6181 gaagccgagc acgatcagca gcacccacag cgatgcgagc agataccagc ggccgcgcga
     6241 actgcgcacg aacttcacga tgttgcctcc cggttgacga gcacgacctc gacggcgacg
     6301 ccgagttcga gtgcctggtc gatcatctct gcggtgcctc cgcggccgcg cgcgggtgcg
     6361 ccgtcccaca gcgcgatcac cacgtcgctc gtggcgacca tctcgcgacc ggcagtctcg
     6421 taggcacgct ggcgggtggg gtcggcaggg tctgacggca cgatnnnnnn nnnnnnnnnn
     6481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6601 nnnncggtcg gccccttcgg ccaagctgga cacgaccacc accgacgcag catcagccgc
     6661 gtccaacgcg ggccacaggc ggtcgagcac cgcgtcgaca caggcctgca cctggtctgg
     6721 atcgaggtac gtccgatgcc cggtgacacc gatgcacaac gcggaaggtg tcttgccgaa
     6781 gtcgtcgatg cccgcgtcgc caatgccgat gtcgctcatc aactcgcaac cttgctgccg
     6841 cggggttgct tcgcgccacg tcgaccggct ggcggtggcg ggccgagcgc ctcgaccacc
     6901 ttgcgggccg taccgagcgc agcgccgtag gtcaggtcgg aacccatcgt ccagatcgcg
     6961 gacaccgacg cgcgctgcac gtcgtcgggc tggtgccagc cggcgtactg gttccacacg
     7021 gccgcgtgcg cacgcccccg cgccgtgggc atcggtggtg tcatcagcga gatcgcgatg
     7081 gccccgatga tcccggcgac agcggtgagg accgccagca cgccgatgcc gtcgaagacg
     7141 acgatcgcga tgacggcgaa ggcgatcgcg agcgggatca tggcgacggc gagtggccgc
     7201 cgtcgtatca atcccaggtt ggctccctcc atctgcagcc cggagcgcag cgatgctgtg
     7261 gcggtgacgc ccgccgaacc ccacagccgc ggcccgacat agcgccgctc accggcgacc
     7321 gactgggatg cccgcgtgcc gtagacgacg agttggtcga gcaccgcccg ctcgaacgga
     7381 ccctgcagct cagcgctgtc gggcacgaac agcgtcaggc gccgctcggt acccgcgatc
     7441 gagatcgcgc cccgctcgcc catgctcaac agggcggccg ctgttgcccg ctggacgcgg
     7501 ttgcgagcgg gttcggtcac gagcgcgacg gcaacgggcg tcaggtcgtc gggacgttct
     7561 gcggtgctgg gcctggggac ttctccgccc acgccgtcgg gctgcccccg gcggcggatg
     7621 ccaccgaacg ggatgtgaga caccaggccg tgcgcgacga gcacaacggc gatcagcgcc
     7681 aacaccgtca tcacgaccga ccagaacacc gacggcccac cgccgtcacg cccgacggcg
     7741 gtgaacgatc gcgcaccgtc gtcgatgctc tcgatcaccg cgtcgatctc gaccgccgac
     7801 gcgccgctgc tgcgggcaac gtccggtacg gcatcggcag ggaatgtggc gatcaacgcc
     7861 accgcatcgc cgcgtgtcac cgcttggagg acgagcgggc cggacgcggc gccagtgtcc
     7921 gtgctggcgt cgtgatgcgt ccgcacgtga cggcgatcga gggtgttgct caaccagacc
     7981 gtgccggtgt cggcgatcgt gctcggcgcc tccgtgtaca gcgcgatcgt cacgggcact
     8041 tcgccatagc cggcgtcgct gagcgagcac cgcgggcagc cgaacagcga gagatcgctg
     8101 tccaacgtgc ggttcgatcc ggtgggtccg cccagcgtca ccacggccgc gtcggcgaag
     8161 cgctggacgt ggcccgacag ttcgtaggtg atcgtcacag acccgtcgac cgtcaggtcg
     8221 gcgtagcgag cgccgtcgag cgcgatcggc ttgccgtcca cggccgcgaa tcgagcacca
     8281 ggtgggacgc gcagccggaa cgtccgcggc gtggcgtcga ggtcgagttg gtagcgcacg
     8341 gcaagcgtgc cgtcgcggcc a
//
LOCUS       DAORNF010000031         8345 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_6307_length_8345_cov_1.376478, whole genome shotgun sequence.
ACCESSION   DAORNF010000031 DAORNF010000000
VERSION     DAORNF010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 8345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8345
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6307_length_8345_cov_1.376478"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..786
                     /locus_tag="PLV68_02225"
     CDS             <1..786
                     /locus_tag="PLV68_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00084.1"
                     /translation="IGDYRVGDLDGVGSNFQTALTIAAALLAIGAVSMFQPRLGRWGA
                     GMAMGAGIALVPFVGYAVRFTFWMSDRARDQAEIVAAAGSGTYVERTLTPGFWLLVAA
                     AVAVVLLIVVALAVLQGDEREPLPTAVSVLGAIACVAAVAGQLIPVAGQRFGDVWSPR
                     FGGRLLVVPRLGMFAVVALVAVIGFLLASRFGLGLAIGASSLYVWQWVSSQFDLGVSP
                     QTPAMYPPGVAHPHLVTTIGAVGIIAAALTASIIATHRDTQSR"
     gene            complement(915..>1755)
                     /locus_tag="PLV68_02230"
     CDS             complement(915..>1755)
                     /locus_tag="PLV68_02230"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRE00085.1"
                     /translation="EREQALRRALDDGDVVAFFQPEIDATTGHVVGAELLARWLRRDG
                     TVVPAAEFLASATDAGLLERLTETIMQQARPVIRRLSTLGLPEGFRFRVNLPPRATER
                     SWRDNPLDLLLQGVDPKLLTVDVTEAAVTDDLAAAAANLAALRVRGIRVCLDDFARGV
                     SSLSLLRKLPLDEVRIDRNSIDTIAAHPHDRAIVRSIIGLVREIGLTATADGVETGAQ
                     ADVLIALGCVRHQGFLYSRALPASEFDSYLAESMAERFAIEAGLVNADRTTLPAWPSD
                     PRP"
     assembly_gap    1756..1786
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1787..3044)
                     /locus_tag="PLV68_02235"
     CDS             complement(<1787..3044)
                     /locus_tag="PLV68_02235"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HRE00086.1"
                     /translation="MTLDSSGGTRRNASTAPVHLDIAGQTPPHAHPHDAPPVPHDHLD
                     NESLARTAPMLLKSHLASAFGIGFMVAATASSVDTARLIAWIAAAVTAEVLSIVAMAS
                     FRNNLAAGVPIRSLAYVRAATFAVGLLWGTSLLMPTRGDTTSMLTHLVFAYGASAFGL
                     LLTSARSDLHLCFQVPLIGVATYALLTSGDGRLMVIGAFGSLYFAASVMVHNAANRSS
                     IGVIETAWRTNYLVENLAAEHDLLVTANTAAAELNRLLTHQATHDPLTGLFNRRGAID
                     ALGTALAAASPSAPVGLLYLDLDRFKHINDTLGHRGGDQFIAVVADRLGRSLDPGATA
                     GRIGGDEFIVVLPRCDIGASMAVATRISSVLGQPVHAEGREVPSSVSVGIAVAPEHGT
                     NSSDLLRFANAALHRAKGAGRNRVDVF"
     gene            3337..>4245
                     /gene="gluQRS"
                     /locus_tag="PLV68_02240"
     CDS             3337..>4245
                     /gene="gluQRS"
                     /locus_tag="PLV68_02240"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HRE00087.1"
                     /translation="MGRFAPSPTGRLHLGNLRTAMVAWLSARHHGGQFLVRMEDLDRV
                     TSSTAHEAAQLADLAALGLDWDGPVVRQSERFGLYDDAIARLDAAGRLYECFCTRREI
                     REAASAPHGDLTAGDAYPGTCRRLSAAGREARRAEGRPPALRLDAEGEVVQVADDVLG
                     VIRLAVDDVVVRRNDGVPAYNLAVVVDDAAQGVTEVVRGDDLATSTPRQVLLQRLLGL
                     VTPRYRHVPLVLGGDGQRLAKRHGAVTLADMADAIGLGIDAVAVQVRSLLAVSIGVAE
                     PGETVTMGVLAERFDLAHLPREPWVLP"
     assembly_gap    4246..4417
                     /estimated_length=172
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4418..4921
                     /locus_tag="PLV68_02245"
     CDS             <4418..4921
                     /locus_tag="PLV68_02245"
                     /inference="COORDINATES: protein motif:HMM:NF025024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HRE00088.1"
                     /translation="IYATRFWTPRFCRAIIRAAEAVGAFEPQPDDPVPGHEVSLAVIS
                     PRLFEAVQADIGVRLWPLLQQAWPCIDYHGLRDAFVIRYALGEQESLRIHHDVAQVSA
                     SIKLNDDYEGGELEFPRQGVTNAAVPVGSVLAWPSLVTHPHQTNQLRSGVKYALTIWC
                     ELPAYLE"
     assembly_gap    5029..5039
                     /estimated_length=11
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5133..>5378
                     /locus_tag="PLV68_02250"
     CDS             5133..>5378
                     /locus_tag="PLV68_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00089.1"
                     /translation="MHLSAVPLTSRARIAAALVGVSVACLSLGACAIDTTVEAAEPRF
                     SGPVPIAPVVAGVAVSPEVVEAVSVAEASAAEVATPEV"
     assembly_gap    5379..5524
                     /estimated_length=146
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5525..6189
                     /locus_tag="PLV68_02255"
     CDS             <5525..6189
                     /locus_tag="PLV68_02255"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HRE00090.1"
                     /translation="EWVGAFPTPIPVVGKSNGPDTQVVQMRLLQLGFWLQATDGKYGV
                     TTRQAVMAFQKYVGLPANGEVDEATATALTDLRIKAHGMTDAGTLIEVDKTKQLLFAV
                     TDGKTQWVFNTSTATGQPYDEPDKNTPGKIQRGVSITPNGLWKTDRERAEGWWEGDLG
                     EIYRPKYFKGGVAIHGSNSIPNYPASHGCVRVSVPAMDFIWDSGMIPLRTTVWVHGEF
                     PG"
     gene            complement(6227..>6751)
                     /locus_tag="PLV68_02260"
     CDS             complement(6227..>6751)
                     /locus_tag="PLV68_02260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00091.1"
                     /translation="PAGEVLRSFDAGAALAVGWTEGYLRGTQAERCFDPVTGLCTVEV
                     FRLRLNQVFDQCRSIGLEPNWLYRVVVVDTELGLNGESGRGLGRFEADAAMVVLAGLL
                     QRHFTTGETISRAGGRIFVLANNTDDLHDRASTISALARDRSLLRAVRVIAWIEDLPA
                     TVRQLDDYIADLSV"
     assembly_gap    6752..6928
                     /estimated_length=177
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7109..>7546)
                     /locus_tag="PLV68_02265"
     CDS             complement(7109..>7546)
                     /locus_tag="PLV68_02265"
                     /inference="COORDINATES: protein motif:HMM:NF012575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HRE00092.1"
                     /translation="NFSFDIQRELVETVVAQRAVDWSNALFLSCRFRAWREGAFNEYL
                     YNFFKSLSAERMRRAEAEARRKLDPPDSDEEITLGGYVMERLCPHRQADLSVFGEIED
                     GTLICTLHGWKFDLESGRCLTADDRHLRVRRADGHPTSSEQPG"
     assembly_gap    7547..7575
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7576..>8345)
                     /locus_tag="PLV68_02270"
     CDS             complement(<7576..>8345)
                     /locus_tag="PLV68_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00093.1"
                     /translation="EVARGDGLTVAIHVETSITDGPGGDSALVVSDGETRLVNQNDCR
                     THDLAALGAHGPIDLHWLQYSGAIWYPMVYDVAPDEMRRLVDAKVDSQFARAMRYVET
                     LGARAVVPSAGPPAFLDPDLYQFNVIRGDEPSIFPDQRSFMARLAAGGHTGVLAIPGT
                     TIEITPAAITATHPMPDAEVAAIFTDKDAYLRRYQADWIGWLDEMKAGWSPPTPDLVT
                     TLQRWWEPLLAMAPTLRHAVGANCLLRFGDLHLVIDFP"
BASE COUNT         1166 a         2567 c         2794 g         1252 t
ORIGIN      
        1 atcggtgact atcgagtggg tgacctcgac ggcgttggca gcaatttcca gacggcactg
       61 acgattgcgg ccgcactgtt ggcgatcggc gcggtgtcga tgttccaacc caggctcggc
      121 cgatggggag caggcatggc gatgggagcc gggatcgcgc tggtgccctt cgtcggttac
      181 gccgtgcggt tcacgttctg gatgagcgat cgcgcccgcg accaggccga gatcgtcgcc
      241 gctgctggca gcggcaccta cgtcgaacgc acgttgacac cgggcttctg gctgctggtc
      301 gcagccgctg tggcagtggt gctactgatc gtcgtcgcgc tcgcggtgtt gcagggcgac
      361 gagcgcgaac cgctgccgac cgcggtcagc gtgctcggag cgatcgcctg tgtcgcagcc
      421 gtggctggtc aactgatccc cgtcgccggg cagcgcttcg gcgacgtctg gtcaccacgc
      481 ttcggcggac ggctgctggt ggtgccgcgc ctggggatgt tcgccgtcgt cgcgctcgtg
      541 gcggtgatcg ggttcttgtt ggcgagccgg ttcgggctcg gtctggcgat cggcgcatcc
      601 agcctgtacg tgtggcagtg ggtgtcgtcg cagttcgacc tcggtgtcag cccgcagacc
      661 ccggcgatgt atccacccgg cgtcgcccac ccccacctgg tcaccacgat cggcgccgtt
      721 gggatcatcg ccgccgcgct cacagcaagc atcatcgcca cccaccgcga cacccagtct
      781 cgctgactgc gctcgattca cccccgtggc aggggtgaac tgaacacagc cgtgggcgga
      841 gccgcagtgt gtgttcgatt gaccccggga ggcggggtga actgaacacc gtcggggtcg
      901 cagcggttcg ggtgtcaggg gcgggggtcg gagggccagg cggggagggt ggtgcggtcg
      961 gcgttgacca gaccggcttc gatcgcgaac cgctcggcca tcgactcggc caggtagctg
     1021 tcgaactcgg acgcgggcaa cgcccgcgag tacaagaagc cctgatggcg gacgcagccg
     1081 agtgcgatca acacgtcggc ctgggcgccg gtctcgacgc cgtcggcggt ggcggtcagg
     1141 ccgatctcgc gcaccaaccc gatgatcgag cggacgatcg cccggtcgtg ggggtgggcg
     1201 gcgatcgtgt cgatcgagtt gcgatcgatg cgcacctcgt cgaggggcag cttgcgcagc
     1261 aggctcagcg acgacacccc acgggcgaag tcgtcgaggc acacgcggat gccgcgcacg
     1321 cgcaacgccg ccaggttggc cgcggccgca gccaggtcgt cggtgacggc cgcctcggtg
     1381 acgtcgacgg tcaacagctt gggatcgacg ccttgcagca gcaggtcgag agggttgtcg
     1441 cgccaactgc gctcggtggc ccgcggcggc aggttgacgc ggaaccggaa gccttccggt
     1501 aggccgagtg tcgacaagcg gcggatcacg ggacgggcct gctgcatgat ggtctcggtc
     1561 agacgttcga gcaggccggc gtcggtggca gacgcgagga actcggctgc gggcaccacc
     1621 gtcccgtcgc ggcgcagcca ccgggccagc agttcggcgc cgacgacgtg gccggtggtc
     1681 gcatcgatct ccggttggaa gaacgcgacg acgtcaccgt catcgagcgc gcggcgcagc
     1741 gcctgctcgc gttcgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncgaa gacatcgacc
     1801 cggttgcgtc ccgccccctt ggcacggtgc agcgccgcgt tggcaaagcg gagcagatcg
     1861 ctgctgttgg tgccgtgctc gggtgcgacc gcgatcccga cgctgaccga cgacggcacc
     1921 tctcgtcctt cggcgtgcac cggctgaccg agtaccgacg agatgcgcgt cgccacggcc
     1981 atcgaagcac cgatgtcgca gcggggtagc acgacgatga actcgtcgcc accgatgcgt
     2041 ccggcggtgg cgccagggtc gaggctgcgg ccgaggcggt cggcaaccac ggcgatgaac
     2101 tggtcgccgc cgcggtgacc gagcgtgtcg ttgatgtgct tgaagcggtc gagatcgagg
     2161 tagagcaacc cgaccggcgc actcggtgac gcggcggcca gcgcagtgcc aagggcgtcg
     2221 atcgccccgc ggcggttgaa cagcccggtc agcggatcgt gcgtggcctg gtgggtgagc
     2281 aggcggttga gctcggctgc tgccgtgttg gcggtgacga gcaggtcgtg ttcggcggcc
     2341 aggttctcga ccaggtagtt ggtgcgccac gccgtctcga tcacgccgat cgaggaccgg
     2401 ttggcggcgt tgtggaccat gaccgaggcg gcgaagtaga ggctcccgaa ggcgccgatc
     2461 accatcagcc gcccgtcacc gctggtgagc agggcatagg tggcgacacc gatcagcggc
     2521 acctggaagc agaggtggag gtcggaccgg gcgctggtca acaacagtcc gaacgcgctc
     2581 gcgccatagg cgaacaccag gtgggtgagc atgctcgtgg tgtcgccacg ggtgggcatc
     2641 aacagcgagg tgccccacag caacccgacg gcgaaggtgg cggcgcgcac gtacgccaga
     2701 ctgcggatcg gcaccccagc ggcgaggttg ttacggaagc tggccatcgc gacgatcgac
     2761 agcacctctg ccgtgacggc ggccgcgatc caggcgatca gccgcgctgt gtcgacgctg
     2821 gacgccgtcg ctgccaccat gaacccgatc ccgaatgcgc tggccagatg gctcttgagc
     2881 agcatcggtg cagtgcgggc cagcgattcg ttgtcgaggt ggtcgtgcgg caccggaggc
     2941 gcgtcgtgag gatgggcgtg cggaggtgtc tggccggcga tgtcgagatg gacgggcgcg
     3001 gtcgaagcgt tccgtcgtgt cccgccgctg ctgtccagtg tcaccgattc gcctttccgt
     3061 gcgtggtccc cgtctggctg acctcaccaa tcgtgtgcga tcgaccacgt cgggtaacaa
     3121 cgtgtgagga cagtatcggt cggttccggc gttgtcgaga gtggtttgtt acggattgtt
     3181 tcttccaagc cgattgacca actcgatcgg cggcacactg ccgaggtgcc cagccgcgag
     3241 ccagcgaccg tccccgccca ggtagcgggt cgcccagcga catctccggt gggtggcctc
     3301 gcaacggctt cggtgggtcg cttcgcatcg ccttcggtgg gtcgcttcgc tccctctccg
     3361 accggtcgtc tgcacctggg caacctgcgc accgcgatgg tggcgtggct ctcggcccgt
     3421 caccacgggg ggcagttcct ggtgcggatg gaggacctcg accgggtcac ctccagcact
     3481 gcccacgagg cggcgcagtt ggccgacctt gctgcgctcg gcctcgactg ggacgggccg
     3541 gtcgtgcgtc agagcgagcg tttcggtctg tacgacgacg cgatcgcccg tctcgacgct
     3601 gccgggcggc tgtacgagtg cttctgtacc cggcgtgaga tccgcgaggc tgcttcggcc
     3661 ccgcacggcg atctgacggc gggcgatgcc tatcccggaa cctgtcgccg gttgagcgcg
     3721 gccgggcgcg aggcccgtcg cgccgagggg cgtccgcccg cgctgcggct cgacgctgag
     3781 ggtgaggtgg tccaagtcgc cgacgacgtg ctcggcgtga tccgcctggc tgtcgacgac
     3841 gtggtcgtgc gacgcaacga cggcgtgccc gcgtacaacc tggccgtggt ggtcgacgat
     3901 gccgcccaag gcgtgaccga ggtggtccgc ggggacgacc tggccacgtc cacgccgcgc
     3961 caggtgctgc tccagcgcct gttgggcttg gtgacgccgc gctaccgcca cgtgccgctg
     4021 gtgcttggtg gcgacggtca gcgcctggcg aagcgccacg gcgcggtcac cctggccgac
     4081 atggcagacg cgatcggatt gggcatcgac gcggttgcgg tacaggttcg ctcgctgctc
     4141 gcggtcagta tcggcgtggc cgagccgggc gaaaccgtca ccatgggcgt gctggcggaa
     4201 cggttcgacc tggcccacct cccccgagaa ccgtgggtgc tgcccnnnnn nnnnnnnnnn
     4261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnatc tatgccacac ggttctggac
     4441 cccgcggttc tgccgagcga tcatccgtgc tgccgaggcc gtcggcgcct tcgaaccgca
     4501 gcccgacgac ccggtgcccg gccacgaggt ctccctggca gtgatcagcc cgcggctgtt
     4561 cgaagcggtg caggccgaca tcggcgtgcg gctgtggccg ctgctgcagc aggcgtggcc
     4621 gtgcatcgac taccacggcc tgcgcgacgc gttcgtcatc cgctacgccc tcggcgagca
     4681 ggagagcctg cgcatccatc acgacgtggc ccaggtatcg gcctcgatca agctcaacga
     4741 cgactacgag ggcggtgaac tggagtttcc ccgccagggt gtgaccaacg cggccgtccc
     4801 ggtcggttcc gtgctggcgt ggccgtcgct ggtcacccac ccgcaccaga ccaaccagct
     4861 ccgctccggc gtcaagtacg ccctcaccat ctggtgcgag ctccccgcct atctcgaata
     4921 gagaccgtgc agggcgcctc ccgcgccggc ccgataccgc cccatggcgg ccgttttcgg
     4981 acgacttcgc gtgccccgcg cggaacgttc gcggattgtc atcggtttnn nnnnnnnnnc
     5041 gatcgcgccg gtgacccccc gttaaggttt gaacaagcct cggcgggcgc ggcacagggt
     5101 gtgccagcca acgccggagc cgtcccgtca ccatgcatct gtccgctgtt ccgctcactt
     5161 ctcgcgcccg gatcgccgcg gcgctggtcg gcgtgtccgt ggcctgcttg tcgctcgggg
     5221 cgtgtgcgat cgacaccacg gtcgaagcgg ccgagccgcg cttcagcggt ccggtgccga
     5281 tcgccccggt ggtcgccggc gtcgcggtca gccccgaagt ggtcgaggcg gtgtcggtcg
     5341 ccgaggcatc agccgccgag gtcgcgaccc ccgaggttnn nnnnnnnnnn nnnnnnnnnn
     5401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5521 nnnnaggagt gggtcggcgc gttccccacg ccgatccccg tcgtcggcaa gagcaacggc
     5581 cccgacaccc aggtggtgca gatgcgtctg ctgcaactgg gtttctggtt gcaggccacc
     5641 gacggcaagt acggcgtgac gacccgccag gcggtgatgg cgttccagaa gtacgtcggg
     5701 ctgcccgcca acggcgaggt cgacgaagcc accgccacgg cgctcaccga cctgcgcatc
     5761 aaggcgcacg gcatgaccga cgcgggcacc ctgatcgaag tcgacaagac caagcagctg
     5821 ctgttcgcgg tgaccgacgg caagacccag tgggtgttca acacctcgac cgccaccggt
     5881 cagccctacg acgaacccga caagaacacc cctggcaaga tccagcgcgg tgtgtcgatc
     5941 accccgaacg ggttgtggaa gaccgaccgc gagcgggccg agggctggtg ggaaggcgac
     6001 ctgggcgaga tctaccggcc caagtacttc aagggtggcg tggccatcca cggctcgaac
     6061 agcatcccca actacccggc ttcacacggc tgcgtgcgcg tgtccgttcc ggcgatggac
     6121 ttcatctggg actccggcat gatccccctc cgcaccaccg tgtgggtcca cggcgagttc
     6181 cccggctgac cgtcacgatc gcccgcatct gattgcccag gtggggtcag acgctgaggt
     6241 cggcgatgta gtcgtcgagt tggcgcactg tggcggggag gtcttcgatc caggcgatga
     6301 cgcgcacggc gcggagcagg gagcgatctc tggcgagggc cgagatggtg ctggcgcgat
     6361 cgtggaggtc gtcggtgttg ttggcgagca cgaagatccg cccgccagcg cggctgatgg
     6421 tctcgccggt ggtgaagtgc cgctgcaaga gcccggccag caccaccatc gccgcgtcgg
     6481 cttcgaagcg gccgagacca cgaccactct cgccgttgag gcccagctcc gtgtcgacga
     6541 ccaccacgcg gtagagccag ttcggttcga gcccgatcga acggcattgg tcgaacacct
     6601 ggttcaggcg cagccgaaac acctcgacgg tgcacagccc ggtgaccggg tcgaagcagc
     6661 gctcggcctg ggtgccgcgc aggtatccct cggtccagcc gacggccagc gcggctccgg
     6721 cgtcgaagct acgcagcacc tccccagccg gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6901 nnnnnnnnnn nnnnnnnnnn nnnnnnnngc ggcccagact gcctgctcga tcgatcgcgt
     6961 cgacccatgc tgcgtgcaca tgtggccaag ggttgggcac atgttggggt cgggcgcggt
     7021 ggctccattg cacaccgaca agacgtggga gaacgccacg catgacgcat cgcgtgacgc
     7081 gctggccgtc ggcgcaccgc ggcgtgtgct atcccggctg ctcggacgat gtgggatgac
     7141 cgtcagcgcg gcggacgcgc aggtggcggt cgtcggcggt gaggcaacga ccggactcga
     7201 ggtcgaactt ccagccgtgc agggtgcaga tcagcgtgcc gtcttcgatc tcgccgaaca
     7261 cgctgagatc ggcctggcga tgcgggcaga ggcgctccat cacgtacccg ccgagggtga
     7321 tctcctcgtc ggagtccggc gggtcgagct tgcgccgggc ctcggcttcg gcgcggcgca
     7381 tccgttcggc cgacaggctc ttgaagaagt tgtagaggta ttcgttgaac gcgccctcgc
     7441 gccaggcccg gaaccggcac gacaggaaca gcgcgttgct ccagtcgacc gcccgctgcg
     7501 cgaccaccgt ttcgaccagc tcgcgctgga tgtcgaacga gaagttnnnn nnnnnnnnnn
     7561 nnnnnnnnnn nnnnncggga agtcgatcac caggtggagg tcgccgaagc gcaacaagca
     7621 gttggccccg acggcatggc gcagggtggg ggccatcgcc agcagcggtt cccaccagcg
     7681 ctgcaacgtg gtgacgagat cgggggtggg cgggctccag ccggccttca tctcgtcgag
     7741 ccagccgatc cagtcggcct gatatcggcg cagataggcg tccttgtcgg tgaagatcgc
     7801 ggcgacctcg gcgtcgggca tcggatgggt cgctgtgatc gccgctgggg tgatctcgat
     7861 ggtggtgccg gggatcgcca acacgccggt atgaccaccc gcggcgaggc gggccatgaa
     7921 cgagcgctgg tcggggaaga tgctgggctc gtcgccacgg atcacgttga actggtacag
     7981 gtcggggtcg aggaatgccg gtggaccggc gctgggcacc acggcgcgag cgccgagggt
     8041 ttcgacgtag cgcatcgctc gcgcgaactg gctgtcgacc ttggcgtcga ccagccgacg
     8101 catctcgtcg ggcgccacgt cgtagaccat cgggtaccag atcgcgccgc tgtactgcag
     8161 ccagtgcagg tcgatcggtc cgtgcgcgcc gagcgcggcc aggtcgtgcg tgcggcagtc
     8221 gttctggttg accaggcggg tctcgccgtc ggacacgacc aaggccgagt cgccgccagg
     8281 gccgtcggtg atcgacgtct cgacgtggat cgccacggtc agcccgtcgc cgcgggcgac
     8341 ctctt
//
LOCUS       DAORNF010000032         8293 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_6353_length_8293_cov_1.736465, whole genome shotgun sequence.
ACCESSION   DAORNF010000032 DAORNF010000000
VERSION     DAORNF010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 8293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8293
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6353_length_8293_cov_1.736465"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1880
                     /gene="treY"
                     /locus_tag="PLV68_02275"
     CDS             <1..>1880
                     /gene="treY"
                     /locus_tag="PLV68_02275"
                     /inference="COORDINATES: protein motif:HMM:TIGR02401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047470 - (1,4)-alpha-D-glucan
                     1-alpha-D-glucosylmutase activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HRE00094.1"
                     /translation="SGDAAGSPFVAIYRLQLTPSFTLDDAVTVVPYLAALGVSHVYTS
                     PYFTAMPGSTHGYDVTNPTEIDPQLGGEAALARFHAVLARHGMSNVIDVVANHMAIGG
                     RYNAWWWDVLRHGRDSAWAHVFDIDWDRSPFAGKVLLPVLGSPYGDSLAAGHLRVVSG
                     PHDEPEVAYFDHRFPLDPASLHLVPRDDDPHRLHVLLDAQHYRLAWWRVGREQINVRR
                     FFEVDTLAGVRVEDPAVFAATHARIRDLAGTRAVTGLRVDHPDGLADPTGYFHRLRIL
                     APDAWIVAEKILEPGESLPAAWPIDGTTGYDAMRLLTGVFVDPAGRDSLTATWHRVSG
                     DPTSFGLHRLAGKQLAADHLLASETDRLTTIAMRIAERSTGARDTTASMMRDALVAVL
                     VHCPVYRTYVRPSEPVSPQDAAVIEATMAAARTHQRHVDRAVFGLVESLWRGEHDDPE
                     AAEFVVRLQQLTGPVMAKGAEDTAFYRDHRLSALNEVGGDPSRFGVTAAEFHAAMAAT
                     AARHPATMVATSTHDTKRSEDVRLRIALLAAIAEEWDAAVVRWQQINRDARAGIDPAT
                     EYLVYQTLVGAWPIDAERMGACALKAVREAKVATSWVDPNPAYEAALGTFVGAILGDA
                     MP"
     assembly_gap    1881..2063
                     /estimated_length=183
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2076..>2392
                     /locus_tag="PLV68_02280"
     CDS             2076..>2392
                     /locus_tag="PLV68_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00095.1"
                     /translation="MLTALGEAWPTIALADDHTGITKLALTRAVLGLRRRYPATWAGT
                     YTPLALSGRHAGAAIAFNRAERQDGGQSDQRDGAVVVVAATRPVAVARDGWGDTTVEL
                     PPGR"
     assembly_gap    2393..2469
                     /estimated_length=77
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2536..>3020
                     /locus_tag="PLV68_02285"
     CDS             2536..>3020
                     /locus_tag="PLV68_02285"
                     /inference="COORDINATES: protein motif:HMM:TIGR02402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00096.1"
                     /translation="MSEPIAVWAPSAASVALQLRDGSMAMVPDAASGWWHGPTLAPDT
                     DYRFVVDGTALSDPRSPRLPEGPEGWSRTVDHTAFSWTDDAWTGRDIAGGVVYELHVG
                     TFSQAGTFAAAIEHLDHLVALGVAAVEIMPVATFPGRRGWGYDGVGLFAPLEHYGGPA
                     G"
     assembly_gap    3021..3030
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3031..4359
                     /locus_tag="PLV68_02290"
     CDS             <3031..4359
                     /locus_tag="PLV68_02290"
                     /inference="COORDINATES: protein motif:HMM:TIGR02402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HRE00097.1"
                     /translation="DLARLVDACHARGIAVLIDVVYNHLGPVGNVLGRFGPYFTDRYH
                     TPWGAAVDYDGPDSAEVRRFVIDNALSWIVDYHADGLRLDAVHALHDASPTHIVAELA
                     AVVRAAGERLGRRVWLVGESDLDDPALVCRADESSEGGANTSGREWGLDAVWSDDFHH
                     ALHVTLTGEQDGYYGDYSGMADLATALADVYVYSGQYCPSRGEPHGHPAGSTDRARFI
                     GFSQNHDQVGNRALGERLCHLVSFERAQIAAVLTLTAPFTPMLLQGEEWAAGTPFQYF
                     TDFADPAIGQAMRDGRRREFAQFGWRPDDVPDPQDPATFVRSRLDWSELDLPPHAAML
                     AWYRALIDLRAKVADLRDGDPAATFVTHGDEHGDAADAAQGGWLVVQRGSVTVVVNLG
                     PATVIPIAPGTIVLTNDPEAEIGRGGVHVGRDRVTIIQHHPGRDPLESPT"
     gene            4612..>5241
                     /locus_tag="PLV68_02295"
     CDS             4612..>5241
                     /locus_tag="PLV68_02295"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRE00098.1"
                     /translation="MSRFGTTVLATATALTATLGLSVVDPSAAHAASRPAPQVEASSL
                     TYTVKSGDYLFGIAISLNVSIKDLLAVNHLTVESPIYPGDRLAVPPGGTLPASTTART
                     ATTPAASAPAANTPATNAATTYTVANGDFLIGIASRHGVTLKALLAANKMEVTAVIRP
                     GMTLSIPPATLPFVAPKPSATAPAAQAATPSPSANPNVVSNYTVKRGDYL"
     assembly_gap    5242..5341
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5342..5811
                     /locus_tag="PLV68_02300"
     CDS             <5342..5811
                     /locus_tag="PLV68_02300"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HRE00099.1"
                     /translation="PAPTLPIPTAKSAGSSSQSAQSATTGASTAQQGKVATVLAFLQG
                     ELGKPYQFATAGPDAYDCSGLVVAAFRKAGISLPHQSLLQSTKGVAVDFENEPIQAGD
                     LIFQVGSGKTYISHVGIAISSTQWIQAAQTGDVVKVGPIPSDSRIAAVRRVIN"
     gene            5860..>7024
                     /locus_tag="PLV68_02305"
     CDS             5860..>7024
                     /locus_tag="PLV68_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRE00100.1"
                     /translation="MKVALTVNDFLRRAEEVYPARTGIVDEPEQPAESWGSSTYAEMA
                     RRARAMAAGLDAMGIGVGERVAMVSHNSARLLTALFGVSGSGRVLVPINFRLVAAEIQ
                     YIVDHSGARLLLIDPELAGALADVRCERVMTIGAASDAEWMRFDVEPQRWEPDEDATA
                     TINYTSGTTARPKGVQLTHRNLWVNATTFGWHLGVSDRDVYLHTLPQFHFNGWGMTYA
                     VTGMGGTHIILRKVDGPEILRRIDEHGVTLLCGAPAVLNMVLDAAQAYIAAGQPVPGR
                     DRVRVVVAGAPPPTRTIERIETELGWEFTQIYGLTETSPLLTINRTRQEFDALTPAER
                     AQRLGRAGAPAIGIRMAISPDGEVLARGNVVMDGYWNQPVETAAAIRTGPDGVD"
     assembly_gap    7025..7104
                     /estimated_length=80
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7105..7426
                     /locus_tag="PLV68_02310"
     CDS             <7105..7426
                     /locus_tag="PLV68_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-dependent synthetase"
                     /protein_id="HRE00101.1"
                     /translation="IISGGENVSSIEVEDALFSHPEIDEVAVIGVPDEKWGELVMALV
                     VRSPGSTLTEDDVIAYVRTRLAGYKCPKRVEFRDTLARTATGKLQKFRLREPFWAGKD
                     RAVN"
     gene            complement(7469..8014)
                     /locus_tag="PLV68_02315"
     CDS             complement(7469..8014)
                     /locus_tag="PLV68_02315"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HRE00102.1"
                     /translation="MSTIPGYKAGTWTVDTAHSEVGFVIRHLMVSKVRGKFTSFNATF
                     TTGENPLDSTVTATVDLASIDTSNADRDAHIRSADFFDTDSNVEMSFVSTSIREDDDD
                     FVLVGDLTIKGITKSVELELELGGFGPDPYGGYRCGFSAEGEINRNDFGISFNAPLEA
                     GGLMLGEKVKIALEVEAVLNA"
BASE COUNT         1261 a         2828 c         2553 g         1201 t
ORIGIN      
        1 agcggcgatg cggcgggcag tccattcgtc gccatctatc ggctccagct gaccccgtcg
       61 ttcacgctcg acgacgcggt gacggtggtg ccgtacctgg cggcactcgg tgtgagccac
      121 gtgtacacct cgccgtactt cacggcgatg cccggcagca cccacggcta cgacgtgacg
      181 aatccgaccg agatcgatcc gcaactcggc ggcgaggccg cgctggcccg cttccacgcc
      241 gtgctcgccc ggcatgggat gagcaacgtg atcgacgtcg tggcgaacca catggcgatc
      301 ggcgggcggt acaacgcgtg gtggtgggac gtgctgcgcc acggccgcga cagtgcctgg
      361 gcgcatgtgt tcgacatcga ctgggatcga tcgccgttcg ccggcaaagt gctgctgcct
      421 gtgctcggca gtccgtacgg cgactcgctc gctgcaggac acctgcgcgt cgtgtccggg
      481 ccgcacgacg agccagaggt cgcctacttc gatcatcgct ttccgctcga cccggcgagc
      541 ttgcatctgg tgcctcgcga cgacgacccg caccgactgc acgtgctgct cgacgcccag
      601 cattaccgct tggcctggtg gcgggtcggg cgtgagcaga tcaacgtccg ccgcttcttc
      661 gaggtcgaca ccctggccgg ggtgcgggtc gaggaccccg cggtgttcgc ggccacccat
      721 gcccgcatcc gtgacctggc cgggacccgt gccgtcaccg ggctacgggt cgatcatccc
      781 gacgggttgg ccgacccgac gggctacttc caccgcctcc gcatcctcgc tcctgacgca
      841 tggatcgtgg ccgagaagat cctcgaaccg ggtgagtcgc tgccggccgc gtggccgatc
      901 gacggcacca ccggctacga cgcgatgcgg ctgctgaccg gcgtcttcgt cgatcccgcc
      961 ggccgcgact cgctgaccgc cacgtggcac cgcgtcagcg gcgatccgac cagcttcgga
     1021 ctgcaccggt tggccggcaa gcagcttgcc gccgaccatc tgctggccag cgagaccgat
     1081 cgcctcacca cgatcgcgat gcgcatcgcc gagcgctcga ccggcgcccg tgacaccacc
     1141 gcgtcgatga tgcgcgatgc gctggtcgcg gtgttggtcc actgccccgt gtaccgcacc
     1201 tacgtccgcc cttccgaacc ggtgtcaccg caggacgcgg cggtgatcga agcgacgatg
     1261 gccgcggccc gaacccatca gcgacacgta gaccgggccg tgttcgggtt ggtggagtcg
     1321 ctgtggcgcg gcgagcacga cgaccccgag gcggccgagt tcgtcgtccg gctgcaacaa
     1381 ctgaccggcc cggtcatggc caaaggggcc gaggacaccg ccttctaccg cgaccatcgc
     1441 ctctcagcgc tcaacgaggt cggcggcgat ccgtcgcgct tcggcgtcac cgcggccgag
     1501 ttccacgccg cgatggccgc gaccgccgcc cgccacccgg cgacgatggt ggccaccagc
     1561 acccacgaca ccaagcgctc cgaagacgtg cggctgcgta tcgccctgct cgccgccatc
     1621 gccgaggagt gggacgcggc cgtcgtccga tggcaacaga tcaaccgtga cgcacgtgcc
     1681 ggcatcgatc cggcgacgga atatctcgtc tatcagaccc tggtcggggc gtggccgatc
     1741 gatgccgagc ggatgggggc atgcgcgctc aaggccgttc gcgaggccaa ggtggccacc
     1801 tcgtgggtcg atccgaaccc ggcctacgag gccgcgctcg gcaccttcgt gggcgcgatc
     1861 ctgggcgacg ccatgccgga nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnngcgcggg caacgatgct caccgcactc ggcgaggcat
     2101 ggccgaccat cgcgctggcc gacgatcaca ccggaatcac caagttggcc ctcacccgcg
     2161 cggtgctggg cctgcgccgc cgttacccag cgacatgggc ggggacctac acccccctcg
     2221 ccctcagcgg ccgccacgcc ggcgccgcga tcgccttcaa ccgggccgag cgccaagacg
     2281 gcggccagtc cgaccaacgc gacggcgccg tggtggtggt cgccgccacc cgaccagtgg
     2341 cggtcgcacg cgacggctgg ggcgacacca ccgtcgaact tcctcctggc cgnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnc gccgcgatca tgtggcacga agcgattgcg atcttggtcc gagacgtcac
     2521 atccgacggt gacacgtgag cgagccgatc gcggtgtggg caccgagtgc tgcctccgtt
     2581 gcactgcagt tgcgcgacgg gtcgatggcg atggtgcccg atgccgcgag cggatggtgg
     2641 cacgggccaa cgctggcgcc ggacaccgac taccgcttcg tcgtcgacgg caccgcactg
     2701 tccgacccgc gctcgccccg cctacctgaa gggccggagg gctggtcacg caccgtcgat
     2761 cacaccgcct tctcgtggac cgacgacgcg tggacagggc gtgacatcgc cggcggtgtc
     2821 gtctacgaac tgcacgtcgg caccttcagc caggcaggca ccttcgccgc tgcgatcgag
     2881 catctcgacc acctcgtcgc cctcggcgtg gccgcggtcg agatcatgcc cgtcgccacc
     2941 ttcccgggcc ggcgcggctg gggctacgac ggcgtcgggc tgttcgcacc gctggagcac
     3001 tacggcggcc cggcggggtt nnnnnnnnnn gatctggccc gcctggtcga tgcctgtcac
     3061 gcccgcggca tcgccgtgct gatcgacgtc gtgtacaacc atctcggccc ggtcggcaac
     3121 gtgctcggcc gcttcggccc gtacttcacc gaccggtacc acacgccatg gggtgccgcg
     3181 gtcgactacg acggcccgga cagcgccgag gtccgccggt tcgtgatcga caacgcgctg
     3241 tcgtggatcg tcgactatca cgccgacggt ctgcgcctcg acgccgtaca cgccctgcac
     3301 gacgcctcgc cgacccacat cgtggccgag ttggccgcgg tggtccgcgc cgccggcgag
     3361 cgcctggggc ggcgggtgtg gctcgtcggt gagagcgatc tcgacgaccc cgcgctggtg
     3421 tgccgcgcgg acgagagcag cgaaggtggc gccaacacga gtggtcggga gtgggggctc
     3481 gacgcggtct ggtccgacga ctttcaccac gccctgcacg tgaccctcac cggcgagcaa
     3541 gacggctact acggcgacta ttccggcatg gccgacctgg ccacggcgct cgccgacgta
     3601 tacgtctatt cgggccaata ctgcccatcg cggggcgagc cccacggcca tcccgctggc
     3661 tccacggacc gcgcccggtt catcggcttc tcgcagaacc acgaccaggt cggcaaccgc
     3721 gccctcggag aacggctctg ccacctggtg tcgttcgaac gggcccagat cgcggcggtg
     3781 ttgacgctga ccgcgccgtt cacacccatg ctgctccaag gcgaggagtg ggcggctggg
     3841 acaccgttcc agtacttcac cgacttcgcc gacccggcga tcgggcaggc gatgcgcgac
     3901 ggccgccgcc gcgaattcgc tcaattcggg tggcgacccg acgacgtacc cgatccgcag
     3961 gacccggcca ccttcgttcg gtcacgtctc gactggagcg agttggacct gcccccgcac
     4021 gcggcgatgc tcgcctggta ccgcgccctg atcgatctgc gcgccaaggt tgccgacctg
     4081 cgcgacggcg acccggccgc gacgttcgtg acccacggtg acgagcacgg tgacgccgcg
     4141 gacgctgcgc agggtgggtg gctggtcgtc cagcgcggct cggtcaccgt ggtggtcaac
     4201 ctcggcccag ccaccgtgat cccgatcgcg ccgggcacga tcgtgttgac caacgacccg
     4261 gaggcggaga tcggccgcgg cggtgtccac gtgggtcgcg atcgggtcac gatcatccag
     4321 caccaccccg gccgagaccc actcgaatcg ccaacgtgac gaaggtcaca tcactcggaa
     4381 accgccgcca agagcctcgc tggcgtcaag aaattctcaa gatcactcaa gggttgccag
     4441 ggctgcgaat ttcgtggctg acctggcagt ctcgacctcg acccgaggtc tcagtcgaga
     4501 gtcgagatca gacctcaacc ctcgacgtga gggttctccc accgccgact tgagtcacga
     4561 aactgtcaag aagcggttca ggtgcaccgt ggcggtgccg atagctgctg catgtcccga
     4621 ttcggtacca ccgttcttgc gaccgcaact gccctgaccg caaccctcgg gttgagcgtt
     4681 gtcgatccgt cggcagccca tgccgcatct cgacccgcac cgcaggtcga ggcttcgtcg
     4741 ctgacctaca ccgtcaagtc gggcgactac ctgttcggca tcgcgatctc actcaacgtg
     4801 agcatcaagg atctcctggc cgtcaaccac ctcaccgtcg agagcccgat ctaccccggc
     4861 gatcgcttgg cggtcccccc aggcggcacc ctgcccgcga gcacgactgc ccgcacggca
     4921 acgaccccgg ccgccagcgc tcctgcagcc aacactcccg cgacgaacgc agcgaccacc
     4981 tacaccgttg ccaacggcga cttcctgatc ggcatcgcca gccgccacgg tgtgacgctg
     5041 aaggcgctgc tggccgccaa caagatggag gtgaccgccg tgatccggcc ggggatgacc
     5101 ctgtcgatcc ctccggccac gctgccgttc gttgccccca agccgtccgc cacggctcca
     5161 gcggcacagg ctgcgacgcc gtcgccgagt gccaacccca acgtcgtctc caactacacc
     5221 gtcaagcgcg gcgactacct cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5341 ntgcctgccc cgacgctgcc gatcccgacc gccaagagtg ccggctcatc cagccagtcc
     5401 gcccagtcgg ccaccaccgg ggcgagcacc gcgcagcagg gcaaggtcgc caccgtgctc
     5461 gccttcctgc aaggcgagtt gggcaagccg taccagttcg ccacggctgg acccgacgcc
     5521 tacgactgca gcggcctggt cgtcgccgcc ttccgcaagg cgggcatctc cctgccccac
     5581 cagagcctgc tgcaatcgac caagggtgtt gccgtcgact tcgagaacga gccgatccaa
     5641 gctggtgacc tgatcttcca ggtcggttcg ggcaagacct acatcagcca cgtcggcatc
     5701 gcgatcagct ccacgcagtg gatccaggct gcccagaccg gcgacgtcgt caaggtcggt
     5761 cccatcccct ccgactcccg catcgccgcc gtccgccgcg tcatcaactg accgagacga
     5821 ctggcaggtc agctcggcgc ggcgcgtaca gtggcgcggg tgaaagttgc gctgactgtc
     5881 aacgacttcc tccggcgagc ggaggaggtc tacccggctc ggaccggcat cgtcgacgag
     5941 cccgagcaac cggccgagtc gtgggggtcg tcgacctatg ccgagatggc tcgccgggcg
     6001 cgggcgatgg cagccgggct cgacgcgatg ggcattgggg tcggtgaacg cgtcgcgatg
     6061 gtcagccaca actcggccag gctattgacc gcgctgttcg gcgtcagcgg ttccggccgg
     6121 gtactggtac cgatcaactt ccgtctcgtc gcggccgaga tccagtacat cgtcgaccac
     6181 agcggggcgc gactgttgct gatcgacccc gaactggccg gcgcactcgc cgacgtgcgc
     6241 tgcgagcgag tgatgaccat cggggccgcc agcgacgccg agtggatgcg cttcgacgtc
     6301 gagccacagc gctgggagcc ggacgaggac gcgaccgcga ccatcaacta caccagcggc
     6361 accaccgccc gccccaaggg tgtgcagctc acccaccgca acctctgggt caacgccacc
     6421 accttcgggt ggcatctcgg cgtcagcgac cgcgacgtct acctgcacac gctgccccag
     6481 ttccacttca acggctgggg catgacctac gccgtcaccg gcatgggtgg cacccacatc
     6541 atcctgcgca aggtcgacgg ccccgagatc ctgcgccgca tcgacgagca cggcgtcacc
     6601 ctgctctgcg gcgcgcccgc ggtgctgaac atggtgctcg acgcggcgca ggcctacatc
     6661 gccgccggtc agccggtgcc cggacgcgac cgcgtgcggg tcgtcgtcgc cggtgcaccg
     6721 ccccccacgc gcacgatcga acggatcgag accgagctcg gttgggagtt cacccagatt
     6781 tacggcctca ccgagacgtc ccccctgttg accatcaacc gcacccgcca ggagttcgac
     6841 gcgctcacac ctgccgagcg ggcccagcgg ctcggccgcg ccggggcgcc cgcgatcggc
     6901 atccgcatgg cgatctcccc cgacggcgag gtgctcgctc gcggcaacgt cgtgatggac
     6961 ggctactgga accagccggt cgagaccgcg gccgcgatcc gcaccggccc cgacggggtc
     7021 gactnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7081 nnnnnnnnnn nnnnnnnnnn nnnncatcat cagcggcggc gagaacgtca gctcgatcga
     7141 ggtcgaagac gccctgttca gccatcccga gatcgacgag gtcgcggtca tcggcgtgcc
     7201 cgacgagaag tggggtgagc tggtgatggc gttggtggtg cgctcccctg ggtcgaccct
     7261 gaccgaagac gacgtcatcg cttacgtacg cactcgcctg gccggctaca agtgccccaa
     7321 gcgggtcgag ttccgcgaca cgctggcccg caccgccacc ggcaagctgc agaagttcag
     7381 gctgcgcgaa ccgttctggg cgggcaaaga ccgcgccgtc aactgacagc gcggtcttga
     7441 ccgacaactc cgatgggttg gagcgcgctc aggcgttgag gacggcttcg acctcgaggg
     7501 cgatcttgac cttctcaccc agcatcagac cacccgcttc cagcggggcg ttgaacgaga
     7561 tgccgaagtc gttgcggttg atctcgccct cggccgaaaa accgcagcgg tagccgccgt
     7621 aggggtccgg gccgaagccg cccaactcga gttcgagctc gacggacttg gtgatgccct
     7681 tgatcgtcag gtcgccgacc aggacgaagt cgtcgtcgtc ctcacggatc gatgtcgaca
     7741 cgaagctcat ctcgacgttg gaatcggtat cgaagaaatc ggccgagcgg atgtgtgcgt
     7801 cgcggtcggc gttggacgtg tcgatcgagg cgaggtcgac cgtggccgtg accgtcgaat
     7861 cgagtgggtt ctcgccggtg gtgaaggtgg cgttgaacga cgtgaacttg ccgcggacct
     7921 tgctgaccat caggtggcgg atgacgaaac cgacctcgct gtgggcggtg tcgaccgtcc
     7981 aggttccggc cttgtaacca gggatggtgc tcatggggga tgctcctcgt tggctggcag
     8041 aggacgatcc tccgcacgcc gagcaacact aggaccaagt agttgagatg acaactacct
     8101 ggtctggctc gttggtccgg ctcgttggtt cggctcgttg gtacgcgacc agcgtcagac
     8161 cgctgcaccc accgcgcggc cgatcaggct ggtgagcacg aacgccacca gcaacagacc
     8221 cacctgacgg acgatgcccc gccacagcgg ccgcttggtc atgcgcgcca gcccaccacc
     8281 gagcattgcc gcg
//
LOCUS       DAORNF010000033         8169 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_6491_length_8169_cov_1.718018, whole genome shotgun sequence.
ACCESSION   DAORNF010000033 DAORNF010000000
VERSION     DAORNF010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 8169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8169
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6491_length_8169_cov_1.718018"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(72..>291)
                     /locus_tag="PLV68_02320"
     CDS             complement(72..>291)
                     /locus_tag="PLV68_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HRE00103.1"
                     /translation="KFTDSLVLHSNVANIGDVRSLVIHPASTTHSQLTPDEQLSSGVT
                     PGLVRLSVGLESIEDILADLELGFAAIS"
     assembly_gap    292..381
                     /estimated_length=90
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<382..>677)
                     /locus_tag="PLV68_02325"
     CDS             complement(<382..>677)
                     /locus_tag="PLV68_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HRE00104.1"
                     /translation="DFAASGRFPNFTEPDPSYHGLAYFPALGHGAFIIKARVQLLRDL
                     GAAISPFNAFLILQGLETLSLRMERHFANTAKVVEYLQSKPQVTSINWAGLASSP"
     assembly_gap    678..729
                     /estimated_length=52
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<730..1377)
                     /locus_tag="PLV68_02330"
     CDS             complement(<730..1377)
                     /locus_tag="PLV68_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HRE00105.1"
                     /translation="MSSEWGFETRQIHVGGDPDPATGARAVPIYQTTSFEFRNSEHAA
                     NLFALAEIGNIYTRIMNPTQLAVEQRINALEGGVTTAVGLPGTLAVASGQSAATLTFL
                     TLAEAGDHIVASPSLYGGTYNLLHHTLPKLGLEVTFVDDPHDLEQWRAAIRPNTKAFF
                     GEVLANPRNDVFDIEGVSKVAHDNGIPLIVDNTVPTPYLIRPFEWGADIVVHSLTK"
     gene            complement(1530..>1938)
                     /locus_tag="PLV68_02335"
     CDS             complement(1530..>1938)
                     /locus_tag="PLV68_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00106.1"
                     /translation="GLDVDAEPAQVVLHHVDVVAAPRGPIMAAAREIAGTVGLPDGWL
                     SDRNSGKVPEHLDTDVVELSRWPNLTVNVLVPDLLLAMAVIGASECSVEGLRALAAAA
                     GLDNSVEIEVLVAEIYPGRALKSGARTRLDELF"
     assembly_gap    1939..2009
                     /estimated_length=71
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2068..3096)
                     /gene="ilvC"
                     /locus_tag="PLV68_02340"
     CDS             complement(2068..3096)
                     /gene="ilvC"
                     /locus_tag="PLV68_02340"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HRE00107.1"
                     /translation="MAAKVYYEADADPSIIRSRKVAIIGYGSQGHAHALNLKESGVPV
                     VVGLREGSSSAAKAEAAGLTVKSISDAAKWADVIMLTMPDTEQKATYENDIKKHLKKG
                     KALAFAHGFNIRFKRIRPSKLIDVIMIAPKGPGHLVRRTYTEGGGVPCLIAVEQDATG
                     QAQALALSYADAIGGARAGVIETTFTEETETDLFGEQAVLCGGASALVQAGFETLVEA
                     GYQPEMAYFECLHELKLIVDLMYEEGISGMRYSISDTAEYGDVTRGPRVVNAKTKAEM
                     KKILKEIQTGKFAKEWVAESESGRGRFNDLRKAGAEHEIEKVGAELRKMMPFLGAGKQ
                     KVADVSGG"
     gene            complement(3096..3641)
                     /gene="ilvN"
                     /locus_tag="PLV68_02345"
     CDS             complement(3096..3641)
                     /gene="ilvN"
                     /locus_tag="PLV68_02345"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HRE00108.1"
                     /translation="MSVASTATQPAYHILSVLVQNRPGVLARVAGLFARRGYNIFSLA
                     VAPTEDDALSRITIVVDVESAPVEQVVKQLFKLIEVVKISELDPRRSVERELLLATVR
                     VTSDNRGQIVELTNIFEGKILAVGIEAITVSLEGNPDKLDDFEDLLRGYGIVELQRTG
                     RVALPKLEREARLRAVKGKAV"
     gene            complement(3638..>4224)
                     /locus_tag="PLV68_02350"
     CDS             complement(3638..>4224)
                     /locus_tag="PLV68_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRE00109.1"
                     /translation="PDDTILVSGVGQHQMWSSQYWNFKRPYSWVNSGGLGTMGFSVPA
                     AIGAKVGRPEATVWAIDGDGCFQMTAQELVTATLERIPVKVAILNNSYLGMVRQWQEM
                     FYEERYSEVYLSPEVPDFVEWAEAMGCVGIRVDSPEEVQPAIDKANGIDDRPVVVDFR
                     VAASEKVFPMVPAGASNDDLLLPPSQMSRKEFLR"
     assembly_gap    4225..4334
                     /estimated_length=110
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4335..5373)
                     /locus_tag="PLV68_02355"
     CDS             complement(<4335..5373)
                     /locus_tag="PLV68_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HRE00110.1"
                     /translation="MTGAQALIKALEMENTEVIFGLPGGCILPAYDPLLDSSIRHVLV
                     RHEQGAGHMAEGYAHVTGRPGVAMVTSGPAATNMVTPLCDAYMDSIPMVCITGQVATT
                     AIGTDAFQECDTVGITRSVTKHNDLVMTAQDLPLAIRQAFHIATTGRPGPTLVDIPKD
                     CMVNTMDWYWPTDAEVVDSLPGYRPNMKGHPRMIKEAAKLILGSERPVLYVGGGILKA
                     RAAEALRELAELTQIHVVTTLMARGAFPDDHPLCLGMPGMHGTASATTAMQKSDLLIT
                     LGARFDDRITGKIAAFAPEAKVIHVDIDPAEQGKVRRPDVPIVGDCRLVIEEIVRAIR
                     DLLAGGATQADT"
     assembly_gap    5639..5770
                     /estimated_length=132
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5771..6009
                     /locus_tag="PLV68_02360"
     CDS             <5771..6009
                     /locus_tag="PLV68_02360"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRE00111.1"
                     /translation="DFWISEFDSGEGFRESHIAFAASSRELVDAFFAAAVGHGAAVLH
                     EPRLWPEYHPDYYGAFVRDPDGNNVEAVCHTPAP"
     gene            complement(6170..6637)
                     /locus_tag="PLV68_02365"
     CDS             complement(6170..6637)
                     /locus_tag="PLV68_02365"
                     /inference="COORDINATES: protein motif:HMM:NF012948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HRE00112.1"
                     /translation="MQFSEPVTGFDSADVVLGGTAGATTVVVTGSGAVYDIAVTGMST
                     AGTVTASIPAGAAVDAIGTDSLASISLDNTVTYEPPVATTTTLEPATTTTLEPATTTT
                     FVETTTSALVSPTGDVLPATGSGSSSSGVLTAAATLVASGIALMLVRRRVRSV"
     gene            complement(6553..7746)
                     /locus_tag="PLV68_02370"
     CDS             complement(6553..7746)
                     /locus_tag="PLV68_02370"
                     /inference="COORDINATES: protein motif:HMM:NF013636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proprotein convertase P-domain-containing
                     protein"
                     /protein_id="HRE00113.1"
                     /translation="MVVGGAVVIAAVGVGSPSSVLAAPVTATFSYTGPPVPIPDGFNT
                     TGTAPGAPASAAITVADRVGAIVDIDVRFDGDACTADWGATTVGLDHSFVFDLRITLT
                     SPAGTTVTLLNRAGGYGNNLCQTLLDEDAVAPSILSASPEDAPFTGTWTPTGFLSVFD
                     GEDANGVWTLTVQDFYRGEVGSIRALSVIVTTDDGAPSAVTKSVSGSFDEGSEVTYTV
                     TAANHLATATLDNVGDEFVDVLPSGLALVSASATSGTAIADIGTNTVTWNGSIAAESS
                     VTITIVATVTASPGTVISNQGRFAFDPDRDGTSGDLRLTDDPATAVIGDPTEFTVTAI
                     APTVSVEQAAGQVDPATAGPIQFMGGFLATIMSISRCSSASRLPGSTAPMWCSAARRA
                     RPLWW"
     gene            complement(7867..>8169)
                     /locus_tag="PLV68_02375"
     CDS             complement(7867..>8169)
                     /locus_tag="PLV68_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase"
                     /protein_id="HRE00114.1"
                     /translation="IASWPTGEFGGMGLEGAVRLGYSKELAAVADPGERDALFQQMVD
                     RMYEHGKAVSTASYFEIDDVIDPADTRRWITTLLRSVPLRPSWSSRPKKRPNIDTW"
BASE COUNT         1284 a         2568 c         2529 g         1333 t
ORIGIN      
        1 atgaccgacc caccggcaag ggtgttcgat tcacccccac cagaggggcg aatcgaacac
       61 actgtcgcgc gttacgagat tgcggcgaag cccaactcga ggtcggcgag gatgtcctcg
      121 atcgactcca gcccgacgct gaggcggacc agacccggcg tgacgccgct ggacaactgc
      181 tcgtcgggcg tgagctggct gtgggtggtg ctcgcagggt ggatcaccag gctgcgcacg
      241 tcaccgatgt tggccacgtt gctgtgcagc accaacgagt cggtgaactt cnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn ngggctggac gccaacccgg cccagttgat cgacgtcacc
      421 tgcggcttgc tctgcaggta ctcgaccacc ttggcggtgt tggcgaagtg gcgctccatg
      481 cgcaggctca gcgtctccag accctgcagg atgaggaacg cgttgaacgg tgaaatcgcc
      541 gcgccgaggt cgcgcagcaa ctgcacgcgg gccttgatga tgaacgcgcc atggcccagc
      601 gctgggaagt aggccaagcc gtggtagctc ggatcgggct cggtgaagtt ggggaaccgg
      661 ccgctggcgg cgaagtcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      721 nnnnnnnnnc ttggtgaggc tgtgcacgac gatgtcggca ccccattcga acggacggat
      781 gaggtacggg gtcggcaccg tgttgtcgac gatcagcgga atgccgttgt cgtgggcgac
      841 cttgctgacg ccttcgatgt cgaacacgtc gttgcgcggg ttggccaaca cctcaccgaa
      901 gaacgccttg gtgttggggc gaatcgcggc gcgccattgt tcgaggtcgt gcggatcgtc
      961 gacgaaggtg acctccaagc cgagcttggg cagcgtgtgg tgcaacaggt tgtacgtgcc
     1021 gccgtacagc gacggggaag cgacgatgtg gtcgcccgcc tcggccaggg tcagaaacgt
     1081 cagcgtcgcg gccgactggc ccgacgcgac cgcgagcgtg cccggcagac cgaccgccgt
     1141 ggtgacgccg ccctccagcg cgttgatgcg ctgctcgacc gccaactgcg tggggttcat
     1201 gatgcgggtg tagatgttgc cgatctcggc cagcgcgaac aggttggctg cgtgctcgga
     1261 gttgcggaac tcgaagctgg tggtctggta gatcggcacc gcacgcgcgc cggtggccgg
     1321 atcgggatca ccgccgacat ggatctggcg cgtctcgaag ccccactcgc tgctcatcac
     1381 tgctctcctc tatcgatctg cgcgcggtgt ggttcacgcc gcgtcatcgc cgggtgactc
     1441 cccagccgcg cggcaacgat agccgacgaa tcccgactat ttcaatcggg atttacagaa
     1501 acctgtcggg cgcgcgtcga gcgcggggtc tagaacaact cgtcgagccg cgtccgggcg
     1561 cccgacttca gcgctcgacc aggatagatc tccgccacga gcacctcgat ctcgacgctg
     1621 ttgtccagtc ccgccgcggc agccagggcg cgcagaccct cgaccgagca ctcgctggca
     1681 ccgatcacgg ccatcgccag caacaggtcg gggaccagca cgttgaccgt caggttcggc
     1741 caccgcgaca actcgaccac gtccgtatcg agatgctcgg gcaccttgcc cgagttgcgg
     1801 tcgctcaacc acccgtcggg caagccgacc gtgccagcga tctcgcgcgc cgcagccatg
     1861 atcggtccgc gcggtgcagc gaccacgtcg acgtggtgca gcacgacctg ggcgggctcg
     1921 gcgtcgacgt cgagcccgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnng cgacccagcc gcgctcgatg gcatcacgtg
     2041 caatcacgac agacacggat gcgacgatca gccaccgctg acgtcggcca ccttctgctt
     2101 gccggcaccg aggaacggca tcatcttgcg cagttcggca ccgacctttt cgatctcgtg
     2161 ctcggcaccg gccttgcgca agtcgttgaa ccggccgcga ccgctctcac tctcggcgac
     2221 ccattccttg gcgaacttgc cggtctggat ctccttgagg atcttcttca tctccgcctt
     2281 ggtcttggcg ttgacgacgc gcgggccccg ggtgacgtcg ccgtactcgg cggtgtcgga
     2341 gatgctgtag cgcatgccgc tgatgccctc ttcgtacatc aggtcgacga tcagcttcag
     2401 ctcgtggagg cactcgaagt aggccatctc gggctggtag ccggcctcga ccagcgtctc
     2461 gaacccggct tgcaccaggg ccgaggcgcc gccacagagc acggcctgct caccgaacag
     2521 gtcggtctcg gtctcttcgg tgaacgtggt ctcgatcacg cccgcgcgcg ccccaccgat
     2581 cgcatcggcg tacgacaacg ccagcgcctg ggcctgaccg gtggcgtcct gttcgaccgc
     2641 gatcaagcac ggcacgccgc cgccttcggt gtaggtgcgc cgcaccaggt gaccaggacc
     2701 cttgggggcg atcatgatca cgtcgatcag cttcgacggg cggatgcgct tgaagcggat
     2761 gttgaagccg tgggcaaaag ccagcgcctt gcccttcttg aggtgcttct tgatgtcgtt
     2821 ctcgtaggtc gccttctgct cggtgtcggg catggtcagc atgatgacgt cggcccactt
     2881 ggccgcgtcg ctgatgctct tcaccgtcag gccagcggcc tcggccttgg ccgcggacga
     2941 cgacccttca cgcaagccga cgaccaccgg cacgcccgac tccttcaggt tcaacgcgtg
     3001 cgcgtgaccc tggctgccgt agccgatgat cgccaccttg cggctgcgga tgatcgacgg
     3061 atcggcatcg gcctcgtagt acaccttggc tgccatcaga ctgctttccc tttcactgcg
     3121 cgaagccgcg cttctctttc caacttgggc aacgcgaccc ggccggtgcg ctgcagttcc
     3181 acgatgccgt agccgcgcag aagatcttcg aaatcatcca gcttgtcggg gttgccctcc
     3241 aagctgacgg tgatcgcctc gatacccacc gccaggatct tgccctcgaa gatgttcgtg
     3301 agctcgacga tctggccgcg gttgtcggag gtgacccgca cggtagcgag caacaactcc
     3361 cgctcgacac ttcgccgggg gtcgagctcg ctgatcttga cgacctcgat cagcttgaac
     3421 agctgcttga ccacctgttc gaccggtgcc gactcgacgt cgacgacgat cgtgatccgg
     3481 ctcagcgcgt cgtcctcggt gggggcgacg gcgagggaga agatgttgta gccgcggcgg
     3541 gcgaacagcc ccgccacccg ggccagcacg cccgggcggt tctgcaccaa cacgctgagg
     3601 atgtggtacg cgggctgggt agcggtggaa gcgacactca tcgcaaaaat tccttcctgg
     3661 acatctggct ggggggcaac aacaggtcat cgttgctggc gccggccggg accatcggga
     3721 acaccttctc ggacgcggcg acgcggaagt cgaccacaac ggggcggtcg tcgatgccgt
     3781 tggccttgtc gatcgcgggt tgcacctctt cgggcgagtc gacacgaatg ccgacgcaac
     3841 ccatcgcctc ggcccactcg acgaagtcgg gcacctcggg cgacaggtac acctcgctgt
     3901 agcgctcctc gtagaacatc tcctgccatt gacggaccat gccgaggtag ctgttgttca
     3961 agatcgcgac cttgaccggg atccgctcca gcgtcgcggt gaccagttct tgcgcggtca
     4021 tctggaagca gccgtcgccg tcgatcgccc acaccgtggc ttccggacgg ccgaccttgg
     4081 cgccgatcgc ggcgggcacg ctgaagccca tcgtgccgag accaccggag ttgacccagc
     4141 tatagggccg cttgaagttc cagtactgcg acgaccacat ctggtgctgg ccgacgccac
     4201 tgaccaggat cgtgtcgtcg ggagnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnnnnn nnnnacgtgt ccgcctgcgt cgcgccgccg gccagcaggt cgcggatcgc
     4381 gcggacgatc tcttcgatca ccaggcggca gtcgccgacg atcggcacgt cggggcggcg
     4441 gaccttgccc tgctcggctg ggtcgatgtc gacgtggatc accttcgcct cgggcgcgaa
     4501 cgcagcgatc ttgccggtga tccggtcgtc gaatcgggcg ccgagggtga tcaacagatc
     4561 gctcttctgc atcgccgtgg tggcgctggc ggtgccgtgc atgccgggca tgccgaggca
     4621 cagcgggtgg tcgtcgggga acgcgccccg ggccatcagc gtggtgacga cgtggatctg
     4681 ggtcagctcg gccagttcgc gcagcgcttc ggcggcacga gccttgagga tgccgccgcc
     4741 gacgtacagg acgggccgct cggatccgag gatcagcttg gccgcctcct tgatcatccg
     4801 cggatggccc ttcatgttcg gccggtaacc gggcaggctg tcgacgacct ccgcgtcggt
     4861 gggccagtac caatccatcg tgttgaccat gcagtccttg gggatgtcga ccaaggtcgg
     4921 cccgggacgc ccggtggtgg cgatgtggaa cgcctggcgg atcgccaacg gcaggtcctg
     4981 cgccgtcatc accaggtcgt tgtgcttggt gacgctgcgg gtgatgccga ccgtgtcgca
     5041 ctcctggaac gcatcggtgc cgatcgccgt ggtggcgacc tggccggtga tgcacaccat
     5101 cggaatgctg tccatgtacg cgtcacacag tggcgtgacc atgttggtcg cggccgggcc
     5161 gctggtcacc atcgccacgc cgggacgacc ggtgacgtgg gcgtagccct cggccatgtg
     5221 cccagcaccc tgctcgtgac ggaccaacac gtggcggatg ctgctgtcga gcagcgggtc
     5281 gtaggcgggc aggatgcagc caccgggcag gccgaagatg acctcggtgt tctccatctc
     5341 cagggctttg atgagggctt gcgccccggt catcttcacg gtgcctcctg ttggactgtg
     5401 acgggctgaa cgagcggacg gccagtgcgg aactggcccg aaaaacaaaa caacctcccg
     5461 ggttgggagg ttgggcggcg tggacggctg actcagccgc gcacacctac acgattacta
     5521 cgagaatgcc ggtgacagag caggtagcgc gcaacacaag tgcgagtctg gcacggctac
     5581 ccccgttttg caaccggacc cgccggttgc tcgcgcgtgg cacgatggcg gcatgctcnn
     5641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5761 nnnnnnnnnn ccgacttctg gatcagcgag ttcgactcgg gcgaaggctt ccgcgagtcg
     5821 cacatcgcgt tcgccgcgtc cagccgtgaa ctcgtcgacg cgttcttcgc ggccgccgtc
     5881 ggccacggcg cagcagtgct ccacgaaccc cggctctggc ccgaatacca ccccgactac
     5941 tacggcgcct tcgtccgcga ccccgacggc aacaacgtcg aggcggtgtg ccacacaccc
     6001 gccccctgac gctttcgttg ttacgacaac cgaccgcggt ggccacctcg tccgcggtgg
     6061 gagcgcggtt gatggtgcag tacgaagatc ccgtcgtgga gcactggttc catcgtcaac
     6121 aacaacgtcg tcagatcgcg ctcgccggct accgctgtgg gcggtgatgt cagacggaac
     6181 gaacgcggcg gcgcacgagc atcagtgcga tgccggacgc gaccaacgtc gcggcggcgg
     6241 tgagcacgcc gctgctcgac gagccggagc cggtcgcggg cagcacgtct ccggtcgggg
     6301 agaccaatgc cgaggtggtc gtctcgacaa acgtcgttgt cgtggccggt tcgagcgtcg
     6361 tcgtcgtagc cggttcgagc gtcgtggtcg tggcgaccgg gggctcatag gtgacggtgt
     6421 tgtcgagcga gatcgaagcg aggctgtcgg ttccgatcgc gtccaccgcc gcacccgccg
     6481 gaatcgacgc ggtcacggtg ccagccgtcg acatcccggt cactgcgatg tcgtagacgg
     6541 cgccgctgcc ggtcaccacc acagtggtcg cgcccgccgt gccgccgagc accacatcgg
     6601 cgctgtcgaa cccggtaacc ggctcgctga actgcaccgt gaaatgctca tgatcgtcgc
     6661 gaggaacccg cccatgaact ggatcggacc cgccgtcgcc ggatcgacct gccccgccgc
     6721 ctgctcgacg ctgacggtcg gcgcgatcgc ggtcacggtg aactcggtcg ggtcaccaat
     6781 gacagccgtc gccggatcat cggtcagacg taggtccccg ctggtgccgt cgcgatcggg
     6841 atcgaacgcg aacctgccct gattcgagat gaccgtgccc gggctggccg tgacggtggc
     6901 gacgatcgtg atcgtcaccg acgactccgc agcgatcgac ccgttccagg tgaccgtatt
     6961 cgtaccaatg tcggcgatcg ccgtgcccga cgtcgccgaa gctgacacca acgcaagccc
     7021 ggacggcagc acatcgacga actcgtcgcc gacattgtcg agggtggccg tcgcgaggtg
     7081 gttggccgcg gtgacggtgt aagtgacctc ggagccttcg tcgaaactgc cggacaccga
     7141 cttggtcaca gcactcggtg ccccgtcgtc agtggtgaca atgaccgaaa gcgcacgaat
     7201 actgccaacc tcaccgcggt agaagtcttg caccgtcagt gtccagacac cgttcgcgtc
     7261 ctctccgtcg aagacagaca gaaatcccgt cggagtccaa gtaccggtaa acggtgcatc
     7321 ctccgggctt gccgacagga tgctcggggc cactgcgtcc tcatcgagca gcgtctgaca
     7381 caggttgttc ccatagccgc cggctctgtt cagcagcgtg acggtcgtac cagccggtga
     7441 ggtcaaggtg atccggagat caaagacaaa gctgtggtcg agaccgaccg tcgtcgcgcc
     7501 ccagtccgcc gtgcacgcgt cgccgtcgaa acggacgtcg atgtcgacga tcgcgcccac
     7561 cctgtcggcc acggtgatcg cagccgaagc aggcgctccg ggagccgtgc ccgtggtgtt
     7621 gaatccgtct gggatcggga ccggaggacc ggtgtaggag aacgtggccg tgaccggcgc
     7681 agccagcaca ctcgacggcg acccaacgcc cacagcagcg atcaccaccg ccccaccaac
     7741 caccatcgcc ccgcacctcg accaccgcgc ccgcacacca gttccagcca tcgctcgcct
     7801 ccaggattcg catccgccag cgacaacgta gtctcgatcc atcgattgat gtcgggtatt
     7861 gcgcggtcac caggtgtcga tgttggggcg cttcttggga cgggacgacc atgacgggcg
     7921 cagtggcacc gagcgcagca gggtggtgat ccagcggcgg gtgtcggccg ggtcgatcac
     7981 gtcgtcgatc tcgaagtacg acgcggtgct caccgccttg ccgtgctcgt acatccggtc
     8041 gaccatctgt tggaacagcg cgtcacgttc gccagggtcg gccacagcgg ccagttcctt
     8101 gctgtaacca agccggaccg cgccctccag gcccatgccg ccgaactcgc cggtgggcca
     8161 gctggcgat
//
LOCUS       DAORNF010000034         8160 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_6501_length_8160_cov_1.799877, whole genome shotgun sequence.
ACCESSION   DAORNF010000034 DAORNF010000000
VERSION     DAORNF010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 8160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8160
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6501_length_8160_cov_1.799877"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(233..1027)
                     /gene="cas5e"
                     /locus_tag="PLV68_02380"
     CDS             complement(233..1027)
                     /gene="cas5e"
                     /locus_tag="PLV68_02380"
                     /inference="COORDINATES: protein motif:HMM:TIGR01868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HRE00115.1"
                     /translation="MTAHTLVLRLAAPLQSWGSTSQFNRRDTDSEPTKSAIVGLLAAA
                     EGRRRGDPIEDLLLLRFAVRVDQSGSLLRDYHTVSDFRGGDLRSSDVNAKGQQKRNRG
                     KATAVTQRFYVQDAVFIALIEGTQDVLEGLASAVLAPGFPLALGRRACVPTQPILLGC
                     GDGPVWSGELTDVALQLPWQAAAWNKRIHAREAADPTTVRLSFTVDDPNGGDVRHDVP
                     TTFAPKERSFTSRAVTRRWLDVSTGLEPRADGERLVSHDPLALLGW"
     gene            complement(1024..>1545)
                     /locus_tag="PLV68_02385"
     CDS             complement(1024..>1545)
                     /locus_tag="PLV68_02385"
                     /inference="COORDINATES: protein motif:HMM:NF020902.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HRE00116.1"
                     /translation="TAVDDQKAATPGEDAGAGMIGTVEFNSATLYRYATVGVDQLMRN
                     LDGSRSDAADAITRFVDGFVRSMPTGKSNTFANRTLPSLVLVVMRSDQPINLVSAFEQ
                     PVRAGFDSGLMTESADRLIKELEAVDAQWASPAVLTLANYSGSLGASLGASAPFPSLL
                     TAVREAIAEDGDE"
     assembly_gap    1546..1561
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1562..2189)
                     /locus_tag="PLV68_02390"
     CDS             complement(<1562..2189)
                     /locus_tag="PLV68_02390"
                     /inference="COORDINATES: protein motif:HMM:NF020902.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HRE00117.1"
                     /translation="MTIRYIDVHVLQTVPPSNINRDDTGSPKQARYGGVDRARVSSQA
                     WKRATRTHFWAGVDPSEQSTRTKRVVTTVADRLVARTGVDRDAASRMAIALTADLNIT
                     PDKKEKKKGAADAPADPEASYLLFFGLEQIDSLIDQVEDRVAALAELDDDALKKELSH
                     VSAAEHLSKGHPIDVALFGRMVADLTSLNVDAAAQVAHAISTHAVQNEF"
     gene            complement(2220..2777)
                     /gene="casB"
                     /locus_tag="PLV68_02395"
     CDS             complement(2220..2777)
                     /gene="casB"
                     /locus_tag="PLV68_02395"
                     /inference="COORDINATES: protein motif:HMM:TIGR02548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HRE00118.1"
                     /translation="MRRDRHVRYYWDRSPRPDQLPVASAVFRRGASGVPGAVPQMWRY
                     MATIGDDTGANLRDITAEHHALVLFGFHQQSQPQLVHVQGVPLGRAVWQLRSSFSPDA
                     VDRRFINAVTANDLDEVVHHLRGLVLQLRSVSPVLRFDYTQLVKDLGSWQRREQVDGV
                     RLRWGRAYHGAGSHDSDDKKDQERQ"
     gene            complement(2740..3399)
                     /locus_tag="PLV68_02400"
     CDS             complement(2740..3399)
                     /locus_tag="PLV68_02400"
                     /inference="COORDINATES: protein motif:HMM:NF037339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00119.1"
                     /translation="MSAAMVDRARAANGWQLPVEIASLVDEAYGALPAVPDAWRSRAD
                     AARGDADTATSESRGKAQPFRLLRQGDSTAPTLAGLHASANQYLDVDRHVCVRDSDGE
                     SVEVILLRRDDRGYWSLRGDFLGVNGETAREEPSAALGGMVRVRIPSGGRRDGNFLRA
                     LDSVSAPPAEWSSHPWLRHWRVLCLDDQGTAQFGQWRLHYDATFGLSDAERSSCALLL
                     G"
     gene            3409..>3845
                     /locus_tag="PLV68_02405"
     CDS             3409..>3845
                     /locus_tag="PLV68_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00120.1"
                     /translation="MFAVNRLGTAIKCRASVARLKASHDQTWLRQANWSSPLVAMQPT
                     GTLEQQVDRGNVGHEEVRIDVEGLLGNLGCDNDEPGGAHATLGRTELVKNCLSVVRAV
                     SRCEAAVEQHRLPRKAISQHSIHRLCSRDRVANDQDDTAGFRLT"
     assembly_gap    3846..3866
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3867..4490)
                     /locus_tag="PLV68_02410"
     CDS             complement(<3867..4490)
                     /locus_tag="PLV68_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00121.1"
                     /translation="MINDNWHEAVRRRERERASGVDPFGFVDDVYGCVCEDGDDVARD
                     AHAIAPPEWFWGRYRGLLSPNLVATIDQLLFAATRTKFVALRFAGLAGKVVVIDEVHA
                     ADDYMAEFLRELLWWLGSLKAPVVLLSATLFNDQRDRLVEQYVKGAERSLRTVSVDRV
                     GYPQITTAALVGDAAELGVHVCESSREARAIALDVATGLSADADEAMA"
     gene            complement(4487..5590)
                     /locus_tag="PLV68_02415"
     CDS             complement(4487..5590)
                     /locus_tag="PLV68_02415"
                     /inference="COORDINATES: protein motif:HMM:NF037707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
                     /protein_id="HRE00122.1"
                     /translation="MSIQLGDEQVRWLDALWGKSDVNGSVHLLVQHLLDTLAVAEVMW
                     DRFLAPCVRQRLDEIAGGGEGRAFFSWVAGLHDIGKATPIFQVQAESLRSRVQTAGLD
                     LTGLRPNEKRIDWRHEVAGAAALVEELTTAWGPETAVEWVWPLVAGHHGLFPDGGRLA
                     LRREQARQRMNGSNDVAWVGARQVLIHLVTAAAGFQHVTDGQPQQAPSRAEQLAIAGL
                     IVMADWIASGPFLSGTSRLDDCGLMHARRRAERAWDALGLRGGWDRRLDVPDDIYRAR
                     FDRSARHVQALAVDVARAMPGPGLMVVEAPMGEGKTELALAVAEVFASRFGQSGVAVA
                     MPTQATCDPIYLRSSGSNNSASSPRWRCCTVSG"
     assembly_gap    6092..6282
                     /estimated_length=191
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6351..6770)
                     /locus_tag="PLV68_02420"
     CDS             complement(6351..6770)
                     /locus_tag="PLV68_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00123.1"
                     /translation="MPKRNRTRERNRTAVSPTRVEPEISEQERAARMFAASIKAHAVA
                     DRAERDRAKAAAERAEHLARLTATKDAAAAKLKHLRATAAPSAKRLEAEAQYRAAAAD
                     LAEHESGARPTWSPKPATTDQSPDSPTPDDSEDSDSN"
     gene            complement(6841..7242)
                     /locus_tag="PLV68_02425"
     CDS             complement(6841..7242)
                     /locus_tag="PLV68_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00124.1"
                     /translation="MLHPDPRAYRVALVADSIVNEGGAGYDAVALLDDAEFGIIVLPP
                     SDFAVPTIASIVEYVVDDLADYRAKGYVVLIVGHSGLPDFGVWLSHVDGEVARRGIPP
                     FTSFDTAGTHPADLVAVLADLHPSPSASTAH"
     gene            complement(7246..>7883)
                     /locus_tag="PLV68_02430"
     CDS             complement(7246..>7883)
                     /locus_tag="PLV68_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00125.1"
                     /translation="AFVAHCLINQNAKVQEFARSPGVVPGVTERLRAHGYRIQQLPCP
                     EMAFAGVDRWWQGRELYDKANYRRHCQILAANMAAPIAEYYRRGYEVVVIGLDGSPSS
                     GVRYTGKAKNWGGRPHFEDGDYEVVEGMGVWMEELKRGLERRGIPWPRASGMLLDTTD
                     WDEARDLPVCLDELDEFLAGGGLMAEVPAPDDALGDTAGNMPGNMSAAGEG"
     assembly_gap    7884..7902
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7978..>8160)
                     /locus_tag="PLV68_02435"
     CDS             complement(7978..>8160)
                     /locus_tag="PLV68_02435"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRE00126.1"
                     /translation="GTTQYLTFECESDDAVTAATERAIANGARLLHDPYETYYGAFQS
                     VLADPDGNVFRINHFR"
BASE COUNT         1402 a         2615 c         2541 g         1355 t
ORIGIN      
        1 cggccagccg cattgctcca cgacatgttc ccacgatggc tggttcgccg ttgctgtata
       61 gagcagcaac cgaccgtccc gctcggtgtc gagtcgccac agaactcgat cgttcggtcg
      121 ggccgccgga agacctgcca tcgccatcgc gtgcagcact tgagggttga cgacggcacg
      181 ccgcgtctgg cgccgtcggg ggttcagcag tattcgtgag aggtacatac gatcaccacc
      241 ccagcaacgc gagcgggtca tggctgacga gtcgttctcc gtcggcgcga ggttccaggc
      301 cggtactgac gtccaaccag cgacgagtca cagcgcgact tgtgaaactg cgctctttgg
      361 gcgcgaacgt cgtcggcacg tcatgccgca cgtcgccacc atttgggtca tctaccgtga
      421 acgacagccg cacggttgtc ggatcagccg cttcccgagc gtggattcgc ttgttccatg
      481 ctgcagcctg ccagggcagt tgcagtgcta catcagtaag ttcacccgac cacactgggc
      541 cgtcaccgca tccgagcaag atgggttgcg tcggcacaca tgcgcgccgg cccaaggcca
      601 gtggaaagcc aggggccaac actgcggacg cgaggccttc gaggacatcc tgtgtgccct
      661 cgatcagcgc aatgaacaca gcatcttgca cgtagaaccg ctgcgtcacg gcggttgcct
      721 taccacggtt acgtttttgt tggcctttgg cattcacgtc agacgatcga agatcaccac
      781 cacggaagtc gctgacggtg tggtagtcgc gcagcaacga ccctgactgg tcgactctga
      841 cggcgaaccg cagcaagagt aagtcctcga ttggatcgcc acgacgacgc ccttcagcgg
      901 ccgcgagcaa gccgacgatg gccgacttgg tcggttcgct atccgtgtcg cggcggttga
      961 actggctcgt gctcccccac gattggagcg gagcagccag gcgaaggacg agcgtgtgcg
     1021 ctgtcactcg tcgccatcct cagcgattgc ctcgcgaacg gcagtgagca gcgaagggaa
     1081 cggcgcgctg gcgccgagcg atgctccgag cgaccccgag tagttggcca gtgtcaggac
     1141 tgcaggagac gcccactgcg cgtcgacggc ctccaactcc ttgatcaacc gatcggccga
     1201 ctcagtcatc aatcccgagt caaagcctgc ccgcacgggc tgttcgaacg cgctcaccag
     1261 attgatcggc tgatcgctac gcatcacgac caggacgaga ctcggcaacg tccgattggc
     1321 aaacgtgttg gacttcccag tcggcatcga acgaacgaac ccgtcgacga atctggtgat
     1381 tgcgtcggcg gcgtcggacc gcgaaccgtc caggttgcgc atcaactggt caacgccaac
     1441 cgtcgcgtag cggtacaacg tggccgagtt gaactcgacc gtgccgatca tccccgcccc
     1501 agcatcttct cctggagtcg cggccttttg atcgtccacc gctgtnnnnn nnnnnnnnnn
     1561 ncgaactcgt tctgcacagc gtgcgtggag atcgcgtgag caacctgtgc cgcggcgtcg
     1621 acgttaaggc tggtgaggtc ggcgaccatg cgcccgaaga gcgcgacatc gatggggtgc
     1681 cccttcgaca ggtgctccgc tgccgagacg tgagaaagct ccttcttcag ggcgtcatcg
     1741 tcgagttcag ccagcgcggc cacccggtct tcgacttggt cgatcaaact gtcgatctgc
     1801 tcaaggccga agaagaggag atagctggcc tcggggtcgg ccggcgcgtc ggcggctccc
     1861 ttctttttct ccttcttgtc gggcgtgatg ttgaggtcgg cggtgagcgc gatcgccatg
     1921 cggcttgcag cgtcccggtc gacaccagtc ctggcaacga gccggtcagc caccgttgtg
     1981 accacccgct tcgttctcgt cgactgctcg gacgggtcaa cccctgccca gaagtgggtc
     2041 ctcgtcgccc gtttccacgc ctgcgacgag acccgtgccc ggtcaacgcc gccatagcgg
     2101 gcttgcttgg gcgaaccggt gtcgtcgcgg ttgatgttgg acggcggcac cgtctgcagg
     2161 acgtggacgt cgatgtatcg gatggtcatg tcattctcct tgtcgtggtg aagagcagct
     2221 cactgccgct cttggtcctt cttgtcgtcg ctgtcgtgac tgcccgcgcc gtgatacgcc
     2281 cgcccccagc ggagacgcac cccgtcgact tgctctcgac gctgccacga tccgaggtct
     2341 ttgacgagct gggtgtaatc aaaacggagc accgggctca ccgatcggag ctgcagcact
     2401 aaaccgcgta gatgatggac aacttcgtcg aggtcgttcg ctgtgaccgc gttgatgaac
     2461 cgacgatcga ctgcatcggg gctgaacgat gagcgaagtt gccacacggc tcgaccaagt
     2521 ggcacccctt gcacgtgcac caactgtggc tgcgattgct gatgaaaccc gaagagcacc
     2581 agagcatggt gctctgcggt gatgtcacga agattggcac cggtgtcgtc accgatggtc
     2641 gccatgtacc gccacatttg cggtacggca cccggcactc cggacgcccc tcgccggaac
     2701 accgcggacg ccaccggcag ttggtccggt cgcgggcttc tatcccagta gtagcgcaca
     2761 tgacgatctc tccgcatcgg acaagccgaa agtggcgtcg tagtggagac gccactggcc
     2821 gaactgagcc gtgccctggt cgtctaggca gagcaccctc cagtggcgaa gccacggatg
     2881 actcgaccac tcggctggcg gcgcactcac gctgtcgagc gcacgcagaa agttgccgtc
     2941 ccttcttcct ccggagggaa tccgtacccg gaccatgcct ccgagggcgg cgctgggctc
     3001 ttctcgcgcc gtttcgccgt tgacccccaa gaagtcaccg cgcaacgacc agtagccccg
     3061 gtcgtcgcgg cgcagcagga tgacttcgac tgactctcca tcggagtcac gaacgcacac
     3121 gtgccgatca acatcgagat actggttcgc gctggcgtgc agccctgcca aggtaggtgc
     3181 tgtggaatct ccttggcgca agagacgaaa cggctgagcc ttaccgcggc tctcactcgt
     3241 cgccgtgtct gcatcacctc gcgctgcgtc ggcgcggctg cgccaagcgt ccggcacagc
     3301 cgggagggca ccgtaggcct cgtcgaccaa cgatgcgatc tctaccggta gctgccatcc
     3361 gttggccgcc cgggcgcggt cgaccatcgc cgcgctcacc agcaacgcat gttcgccgta
     3421 aatcgcctgg gaaccgcgat caaatgtcgg gccagcgtcg ccaggctcaa ggccagtcac
     3481 gatcaaacgt ggctccgaca agccaactgg tcgagcccgc tcgtggcgat gcagccgacc
     3541 ggcacgttgg agcagcaagt cgaccggggc aatgtcggac acgaggaggt ccgcatcgac
     3601 gtcgaaggac tgctcggcaa tctgggttgc gacaacgacg agccgggcgg ggcgcacgca
     3661 acgctcgggc ggaccgagtt ggtcaagaac tgcctgagcg ttgtccgcgc ggtcagccgc
     3721 tgtgaagcgg ccgtggagca acaccgtttg cccaggaagg cgatcagcca gcactcgata
     3781 catcgcctgt gctctcgcga ccgtgttgcg aatgaccagg acgacaccgc cggcttccgt
     3841 ctcacnnnnn nnnnnnnnnn nnnnnnagcc attgcctcgt cggcatcggc tgacaggccg
     3901 gtggcgacat cgagcgcgat cgctcgtgcc tccctactcg actcgcacac gtgtacgccg
     3961 agctcagcgg catcgcccac caaggcggcg gttgtgatct gcggataccc aacccgatcg
     4021 acagagaccg tccgcagcga gcgctcggcg cctttgacgt actgctcgac aagtcgatcg
     4081 cgctggtcgt tgaacagcgt cgctgacagc agaacaaccg gcgccttcaa ggatccaagc
     4141 caccacaaca gttctctgag gaactctgcc atgtagtcgt ccgcggcgtg cacctcatcg
     4201 atcaccacca ccttccccgc cagccctgcg aaccgaagcg ccacgaactt ggtgcgtgtc
     4261 gcagcgaaca gcagttggtc gatcgttgcg acgaggttcg gggacagcaa accgcgatag
     4321 cggccccaaa accactcggg gggtgcgatg gcgtgtgcgt cgcgcgcgac atcgtccccg
     4381 tcctcgcaca cgcatccgta cacatcgtcg acaaagccaa acggatcgac gccgctggca
     4441 cgctctctct cgcgacgccg gacggcctcg tgccagttgt cgttgatcat ccgcttaccg
     4501 tgcaacaacg ccagcgtgga ctgctcgccg agttgttcga gccactgctg cggaggtaga
     4561 tcgggtcaca tgttgcctgt gtcggcatcg ccacagcgac accgctctgc ccgaatcggc
     4621 tggcgaacac ctctgccacc gcgagtgcaa gttcggtctt gccctcgccc atcggggcct
     4681 cgaccaccat cagaccaggg ccaggcatcg ctcgtgcaac atcgacagcg agcgcctgaa
     4741 cgtgccgagc agaccgatcg aatcgggcgc ggtagatatc atcaggtacg tccaggcggc
     4801 gatcccaacc accgcgaagc cccaacgcgt cccaggcccg ctccgcacgc cgtcgcgcat
     4861 gcatcaggcc acagtcatca agacgagagg tgcccgacaa gaacgggccg ctcgctatcc
     4921 aatcagccat cacgatgagg ccggcgatgg cgagttgctc ggcgcgactg ggtgcctgtt
     4981 gcggttgtcc gtcggttacg tgctgaaacc ccgcagccgc ggtgacgaga tggatcagca
     5041 cttgtcgagc accaacccac gcaacgtcgt tggagccgtt catccgttgc cgagcctgtt
     5101 cgcgccgcag cgccaggcga ccaccatcag gaaagaggcc gtgatggcca gcaacgagcg
     5161 gccagaccca ctccaccgca gtctccggcc cccaggccgt ggtgagttct tcaaccagcg
     5221 cggctgcacc tgcgacctcg tgccgccagt cgatccgctt ctcgttcgga cgtagacctg
     5281 tgaggtcgag acccgccgtc tgaacgcgag aacgcaacga ctccgcttgc acctggaaga
     5341 tcggtgtcgc cttgccgatg tcatgcagac cagccaccca gctgaagaag gctcgccctt
     5401 caccgccgcc agcgatctca tcgagccgct gccgtacaca cggcgcgagg aagcgatccc
     5461 acatcacttc ggcaacggcg agggtgtcaa gcaggtgctg caccaagagg tggacggaac
     5521 cgttgacgtc agacttgccc cacagcgcgt cgagccatcg aacctgctcg tccccgagtt
     5581 ggatcgacac cggaagctgc agatcaggct gaccgttcac gttggaccca cgaccgacga
     5641 acgcggaacc agaccaccgc tactcgggag cgtccatcgc ttcgcgaccc gtggataccg
     5701 acggtgagac gggagcggcg caccccgctc gatgagtcgg tgaagcacct gtcggcatgt
     5761 ctcggcatcg gaacgtgccc gatgcggagc gggctggtcg atgccgagtc gcgagcagga
     5821 ctcccgcaac gacatgagtc cacgtcgccg cggttcgagg accgcagcgg cgagcgcggc
     5881 agtacagatt ccggcaggac gcgatccgag atcgacgccg agccgaccga actcgctgcg
     5941 aagcaccgcc cagtcgaacg agaggtgatg agcaacgatc acgctgtctt ccagcatgtc
     6001 gagaacgcgc cccgcaatct gtgcgaacga tggagcaaga gcgacgtcga tggccgtgat
     6061 gccatgaagc agtgtcgcgg ttaccacacc cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngacgcggt ttgacccgtc
     6301 gtcaagtcca acgtgttgag gcctcgccgg ggaccccagc agacccggct tcagttggag
     6361 tcggaatcct ctgagtcgtc tggggtgggt gagtcggggg actgatcggt cgtggcgggt
     6421 ttgggggacc aggtggggcg agcaccggat tcgtgttcgg ccaggtcggc ggcggcggcg
     6481 cggtactggg cttcggcttc taggcgcttc gcggaagggg ctgccgttgc gcggaggtgt
     6541 ttgagcttcg ccgcggcggc gtctttggtg gcggtcaggc gggctaggtg ctcggctcgt
     6601 tcggcggcgg ctttggctcg gtcgcgttcg gcgcggtcgg cgacggcgtg ggccttgatg
     6661 ctggccgcga acatccgcgc tgctcgttct tgctcgctga tctccggttc gaccctggtg
     6721 ggcgagacgg cggtgcggtt gcgttcacgg gtcctgttgc gctttggcat cggggtcaga
     6781 ccatagaccc cacgtgagcg ccgctccacc cggaaccggc acacgcacgg tgcagcgcgg
     6841 tcagtgcgcg gtgctggctg agggggacgg gtggaggtcg gccaggacgg caaccaggtc
     6901 ggcggggtgg gtgccggcgg tgtcgaagct ggtgaacggg gggatgccgc ggcgggcgac
     6961 ttcgccgtcg acgtggctga gccagacgcc gaagtcgggg aggccggagt ggccgacgat
     7021 cagcacgacg tagcccttgg cgcggtagtc ggccaggtcg tcgacgacgt attcgacgat
     7081 cgaggcgatc gtcggcacgg caaagtcgga agggggcagg acgatgatgc cgaactcggc
     7141 gtcatcgagc agcgcgaccg cgtcgtatcc ggcgccgcct tcgttgacga tcgaatccgc
     7201 taccagagcg acgcgatagg cacgtgggtc aggatggagc accgctcagc cctcccccgc
     7261 tgccgacatg ttcccgggca tgttcccggc cgtgtccccc aacgcgtcgt ccggcgctgg
     7321 aacctcggcc atcaggccgc caccggccag gaactcgtcg agctcatcga gacacacggg
     7381 caggtcgcgc gcttcgtccc agtcggtggt gtcgagcagc atgccgctgg cccgcggcca
     7441 cggaatgccg cggcgctcca ggccccgctt cagttcctcc atccacacgc ccatgccctc
     7501 gaccacctcg tagtcgccgt cctcgaagtg gggtcggccg ccccagttct tggccttgcc
     7561 ggtgtagcgc accccgctgg aggggctgcc gtcgaggccg atcaccacca cctcgtaccc
     7621 gcggcggtag tactcggcga tcggcgcggc catgttggcg gccaggatct ggcagtgacg
     7681 ccgatagttg gccttgtcgt acagctcacg accctgccac cagcggtcga cacccgcaaa
     7741 cgccatctcc gggcacggca gctgctgaat ccggtacccg tgcgcacgca ggcgttcggt
     7801 cacgccgggt acgacacccg gcgagcgggc gaactcctgc accttggcgt tctggttgat
     7861 gaggcagtga gcgacgaagg cgannnnnnn nnnnnnnnnn nngcgtcgac cggcgcctcg
     7921 ggcacggacg gcgccggcac ggacgagacg tcggatgact cgctcaccgc tgccaactca
     7981 ccggaagtgg ttgatccgga acacgttgcc gtcggggtcg gccagcaccg attgaaacgc
     8041 gccgtagtac gtctcgtacg ggtcgtgcag cagacgggcg ccgttggcga tggcccgttc
     8101 ggtcgccgcc gtcaccgcgt cgtcgctctc gcactcgaac gtcaggtact gggtggtccc
//
LOCUS       DAORNF010000035         7931 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_6739_length_7931_cov_1.702895, whole genome shotgun sequence.
ACCESSION   DAORNF010000035 DAORNF010000000
VERSION     DAORNF010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 7931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7931
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6739_length_7931_cov_1.702895"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1640)
                     /locus_tag="PLV68_02440"
     CDS             complement(<1..1640)
                     /locus_tag="PLV68_02440"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HRE00127.1"
                     /translation="MPASAASPPFISPDADWLTTVNYFRAMAFQGPVTENTSWSAGAY
                     NHSCYMLQNGITHTEVAGKPGYTPSGDTAGRNSNVAVSSVYGASARSHIELWMTGPFH
                     AIGVLRPGLTSVGFGKCDMTNPGTNYRSGATLNVLSGLGPSVPQASPILFPGNGTTTS
                     LNMFRTESPNPAVMCGWGATTAGLPVVAMMPEGFSGVPVATMIGASTGPQQVCVLSGA
                     NANDPNDPAGTAKSILNGVNGVVVMPRNTLAPDTYSVTITTSSRTVTWSFTVDPTAAT
                     GLQPVPVTQPLDREGATLKPVSPVRVVDTRSNLGTSKLFSGIAKRVQITGRGSVPVGA
                     SAVSANFTITNAQAGGYLTVWNCSTDRPVVSTLNYSTTSAVPNGASVPLDTSGGLCVF
                     SSATADLVVDVNGYYDDGGDGRFSSVSPARLMDTRETNSRLGKGEIRQLRVAGVASVP
                     FGAEVVSLNVTSVAPDYEGYVTVWPCDVAMPTASSVNPVPGAAKPNVVLAPVAADGTV
                     CFFASTPVDIVVDITGYVASSASLTYVPATPFRLTDTR"
     assembly_gap    1688..1700
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1866..>2546)
                     /locus_tag="PLV68_02445"
     CDS             complement(1866..>2546)
                     /locus_tag="PLV68_02445"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HRE00128.1"
                     /translation="AAIAWTVRDAGDSIIASEGVSTEIVIVEDHATDDSRAVAERYLA
                     AHPDVAMVLLAKDANEGLAKARNTGISECRADLVMMMDADNLVYPTCLARLRDALTAR
                     PDAAFAYSALERFGAEAGLISAFAWNPEWLCAANYIDAQAMLRRDVLVEFGGYRTDDP
                     LVFGWEDWELWLRLASAGRHGVLVPEMLGRYRVQAGSMIGVTNMAVDESLAHLRDLHP
                     DLPWPAIV"
     assembly_gap    2547..2620
                     /estimated_length=74
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2621..>2851)
                     /locus_tag="PLV68_02450"
     CDS             complement(<2621..>2851)
                     /locus_tag="PLV68_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00129.1"
                     /translation="RDVLGRLAAHVASGAHARGTWRLREGGAQGHKRLPAFKPYATVL
                     GRAKRLVLGENEVLRRLEAVRALLHHDAEQHI"
     assembly_gap    2852..2877
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2878..3811)
                     /locus_tag="PLV68_02455"
     CDS             complement(<2878..3811)
                     /locus_tag="PLV68_02455"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRE00130.1"
                     /translation="MTAPVNARVNVPIKVFVAATGNAFMRDIAAAFVEAAQQLGRDAQ
                     LVDDRLPDADGSINFVVAPHEFYVLADADPAALKRAAAASIAICTEQPATPWFHLSLD
                     ACQRGLLAFDINEHGTTALREYGVRAERLPFGAVPSMVAPGAHRADRPVAERPIDVLF
                     MGSLDPTRGAALAGLAPVLWNRHADLRLFPFDKPVRPDSPGVVFGAAKFDVLRSAKVL
                     VNVHRDRSTHLPTGAEPPAYFEWVRMVEAMANGCVVVTEPSEGFAPLVPGEYFVEATA
                     AGMPAVIDDLLADPDRMQHIADAARRAVTEQLPLH"
     gene            complement(3808..4221)
                     /locus_tag="PLV68_02460"
     CDS             complement(3808..4221)
                     /locus_tag="PLV68_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00131.1"
                     /translation="MTGTTPGKPTSNPTTSNSASPRKAAPNKAAPNKVASNKPARASD
                     ESAAAELDRLRTENADLRRRVESAEAAIDELRADALARRSEVRALVADLPAALSRKAL
                     LRQMAADAVHHPDKKGVVVRAVNKARRTARNATRR"
     gene            complement(4218..>4718)
                     /locus_tag="PLV68_02465"
     CDS             complement(4218..>4718)
                     /locus_tag="PLV68_02465"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRE00132.1"
                     /translation="QPGRVLDIGCGIGRNLGHLDGNGVGVDHNPDCVAACRARGLIAF
                     TPDEFATSAEARPAGFDSLLFAHVLEHLTETDAVALVRQYLPFLRDGGRVLVITPQPR
                     GQASDATHVTMFDEPAVRRLAVMAALQVDSIRSFPFPRPVGRVFTHNETVSILSRLPI
                     QGSASS"
     assembly_gap    4719..4739
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4917..>5634
                     /locus_tag="PLV68_02470"
     CDS             4917..>5634
                     /locus_tag="PLV68_02470"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRE00133.1"
                     /translation="MPSTSPSSLSTPPGSLEEPLVTVAIPYYANLAYLAEALASVQAQ
                     SDQRWRALVVDDAGPEPATDLVAALDDARIGVVRNAVNLGLAGNWNRCIALATTPWVT
                     LLHADDRLHRDYVGAVLAAIEQHPDVTAVFTDAAVIGDYGQPRRSLPEFVKRFARRPG
                     VDHRVEGDRSLASILSNNYVMCPTLTYCTEPARALLFDERWSMVMDLDHTASMLLDGQ
                     AMWAIRRPLYEYRRHAANTTN"
     assembly_gap    5635..5701
                     /estimated_length=67
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5833..>6729)
                     /locus_tag="PLV68_02475"
     CDS             complement(5833..>6729)
                     /locus_tag="PLV68_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00134.1"
                     /translation="PRPVVSSLNFEPGQTVPNLVTVKVGANGRVNLFNSAGNTHLVAD
                     IVGYYTTAPNVTGGRFSALNPARLLDTRDGTGVGAAGPISQASAIDLAVVGRGGVPAR
                     GVQAVALNVTVDVPSSSGFLTVWPSGETRPTASTHNFTPGQTIANMVIAKVGANGKIS
                     IFNSAGHTHVIADVVGYFSDQGGLFVPVSPTRQLDTRESSPVAAGESRALRVTTSPIP
                     AHASAVVTNVTSVDASVQSYVTVWPQGADRPVASTLNPRPSVPVPNQAYLRVGSNGEL
                     RLFNFSGTTNLVVDTFGYFTTN"
     assembly_gap    6730..6884
                     /estimated_length=155
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6885..7538)
                     /locus_tag="PLV68_02480"
     CDS             complement(<6885..7538)
                     /locus_tag="PLV68_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00135.1"
                     /translation="MVARVAWRARDNRAGAPLLNWIETQDIVIFPGCHEISLDLVTNP
                     AAGLHDQFSAWFYSPVTKRLEDSGYVTGWRGVNIEALRFDLNEDRGVRGFTLRNVKLA
                     DDAAFSTSYPITFRDQAAAPGTVADIYVTTDERGLNGTQIARGVSVQAGTNTFTWNGT
                     RVDGSSMPNGTYWVYMVMRNTAGVGSGYSTGPVRLERPVPSAPSAYVPLMPARVLDTR
                     "
     assembly_gap    7563..7718
                     /estimated_length=156
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7719..>7931)
                     /locus_tag="PLV68_02485"
     CDS             complement(<7719..>7931)
                     /locus_tag="PLV68_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00136.1"
                     /translation="FRALVAGNPAVNLQIDSVRIHRADAPSSQPSGIPRPQVLTPNEQ
                     GGEDYATAVRGNPWDFAGMDDVGETHD"
BASE COUNT         1177 a         2488 c         2574 g         1180 t
ORIGIN      
        1 tctcgtgtgt cggtcagacg gaacggggtt gcgggcacgt acgtgagcga cgcgctcgaa
       61 gcgacgtacc cggtgatgtc gaccacaatg tcgaccggtg tcgaagcgaa gaaacacacc
      121 gtgccatcgg ccgcgaccgg cgccagcacg acattgggct tggccgcacc cggcaccggg
      181 ttgacgctcg atgcggtcgg catcgcaaca tcgcacggcc acaccgtgac gtagccctcg
      241 tagtcgggcg cgacgctggt gacgttgagg ctgacgactt cggcgccgaa cggcacgctg
      301 gcaacaccag cgacgcggag ctgacgaatc tcacccttac ccaagcgcga gttggtctca
      361 cgggtgtcca tcaaccgggc cggcgagacg gaggagaacc tgccgtcccc gccatcgtcg
      421 tagtagccgt tcacgtcgac caccagatcc gccgtggcag aggagaacac acacagcccg
      481 ccactcgtat cgagcgggac gctcgctccg ttggggacgg ccgaggtggt cgagtagttc
      541 agcgtggaca cgaccgggcg gtccgtggag cagttccaca cggtcagata cccgcccgcc
      601 tgcgcgttgg tgatcgtgaa gttggcgctg accgctgacg caccgacggg caccgaaccg
      661 cgtccggtga tctggacccg cttggcaatg ccggagaaca gcttgctggt accgaggttg
      721 ctgcgggtgt cgacgacacg cacgggcgac acgggcttca gggtcgcgcc ctcgcgatcc
      781 aacggctggg tcaccgggac cggctggagg ccggtggccg cggtggggtc gaccgtgaag
      841 ctccacgtca ccgtgcgtga cgaggtcgtg atcgtcaccg agtaggtgtc aggggccagc
      901 gtgttgcgcg gcatcacgac cacaccattg acgccgttga ggatggactt ggcggtgccc
      961 gccggatcgt ttggatcgtt ggcattggcc cctgagagca cgcacacctg ctgcggtccc
     1021 gtcgaagcgc cgatcatcgt ggccaccggg acgccgctga acccttcggg catcatcgcc
     1081 accacgggca ggccagcggt ggtcgcgccc cacccgcaca tcaccgcggg gttcggcgat
     1141 tccgtgcgga acatgttgag actggtggtg gtgccgttgc cgggaaacag gatcgggctc
     1201 gcctgcggga ccgaagggcc aaggccgctc agcacgttca gcgtggcgcc cgagcgatag
     1261 ttggtaccgg ggttggtcat gtcgcacttg ccgaacccga ccgaggtcaa acctggacgc
     1321 agtacgccga tcgcatggaa cggtccggtc atccacagtt cgatgtggct gcgcgccgac
     1381 gcgccgtaca cgctgctgac ggcgacgttg ctgtttcgtc cggcggtgtc gcccgacggc
     1441 gtatagcccg gcttgcccgc gacttcggta tgggtgatgc cgttctggag catgtagcac
     1501 gagtggttgt aggcaccggc cgaccacgac gtgttctcgg tgaccggccc ttgaaaggcc
     1561 attgcccgga agtagttgac cgtggtcagc cagtcggcgt cgggcgagat gaaaggcgga
     1621 ctggccgcgg aggcgggcat caccatcgat gcgccaacgc ccagtgtcac ggcgaccgcc
     1681 gcgaatgnnn nnnnnnnnnn cccgtcgtac ccgctgaacc atggcctgaa gtccgtctcg
     1741 ccgtgatttc acgccgactc atcggtcagc caccgtggcg tcctgaatcg tttcgggggc
     1801 cgcactcacc ccccgtgaga ctttgaggaa gtcttcacga accgccccgc gaaaccagac
     1861 gggatctaga cgattgccgg ccagggcagg tcgggatgca ggtcacgcag gtgggccagc
     1921 gactcgtcga ccgccatgtt ggtgacgccg atcatcgacc cggcctggac gcgatagcgc
     1981 ccgagcatct cgggtaccag caccccgtgg cgcccggcgc tggccaggcg caaccacaac
     2041 tcccagtcct cccagccgaa caccagcggg tcgtcggtgc ggtagccgcc gaactcgacc
     2101 agcacgtcac ggcggagcat cgcctgcgcg tcgatgtagt tggccgcgca cagccactcg
     2161 gggttccagg cgaacgcgct gatcaacccg gcctcggcgc cgaagcgttc gagcgcgctg
     2221 tacgcgaatg ccgcatccgg gcgggcggtc agcgcgtcac gcaagcgcgc caggcaggtg
     2281 ggatacacca ggttgtcggc gtccatcatc atcaccaggt cggcccgaca ctcgctgatg
     2341 ccggtgttgc gcgccttggc caggccctcg ttggcgtcct tggccaacag caccatcgcc
     2401 acgtcgggat gcgcagccag atagcgctcg gccacggcgc ggctgtcgtc ggtggcgtgg
     2461 tcttcgacga tgacgatctc ggtgctgacg ccctcgctgg cgatgatgct gtcgccggcg
     2521 tcgcgcacgg tccaggcgat ggctgcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tggatgtgct gttcggcgtc
     2641 gtgatgcagc aaggcccgca ccgcttccag gcggcgcagc acctcgttct cgccgagcac
     2701 cagccgtttg gcccgtccga gcaccgtggc gtagggcttg aacgcgggga gccgcttgtg
     2761 gccctgggcg ccaccttcgc gcaaccgcca ggtgccgcgg gcgtgagcgc cgctggcgac
     2821 gtgggcagca aggcgcccca gcacgtcgcg cnnnnnnnnn nnnnnnnnnn nnnnnnncgt
     2881 gcaacggcag ttgctcggtg accgcacggc gggccgcgtc ggcgatgtgc tgcatccgat
     2941 cgggatcggc caacaggtcg tcgatgaccg cgggcatgcc ggccgcggtc gcctcgacga
     3001 agtactcgcc gggcacgagc ggggcgaagc cctccgacgg ctcggtcacc accacgcacc
     3061 cgttggccat cgcctcgacc atccgcaccc attcgaagta ggcaggtggc tcggcacccg
     3121 ttggcaggtg ggtgctgcga tcgcggtgca cgttgaccaa caccttggcc gagcgcagca
     3181 cgtcgaactt ggccgcgccg aagaccacac cggggctgtc gggccgcacc ggcttgtcga
     3241 agggaaagag gcgcagatcg gcatggcgat tccacagcac cggcgccagc ccggccaacg
     3301 ctgcgccgcg cgtcgggtcg aggctgccca tgaacaacac gtcgatcggt cgctctgcta
     3361 ccggccggtc cgcccgatgg gcgccgggcg cgaccatcga cggcaccgct ccgaacggca
     3421 accgctcggc ccgcacgccg tactcgcgca aggcggtcgt gccgtgctcg ttgatgtcga
     3481 aggccagcag accgcgctgg cacgcgtcga gcgacaggtg aaaccagggc gtggcgggct
     3541 gttctgtgca gatcgcgatg ctggccgccg cggcgcgctt cagcgcggcc ggatcggcgt
     3601 cggccaacac gtagaactcg tgcggcgcga cgacgaagtt gatcgacccg tcggcgtccg
     3661 gcagacgatc gtcgaccagt tgggcgtcgc gccccaactg ctgagccgct tcgacgaacg
     3721 cagcggcgat gtcgcgcatg aacgcgttgc cggtcgcggc aacgaagacc ttgatcggaa
     3781 cgttgacgcg ggcgttgacg ggggcggtca tcggcgagtg gcgttgcgcg ccgtgcggcg
     3841 ggccttgttg acggcgcgca ccacgacgcc cttcttgtcg gggtggtgca ccgcgtcggc
     3901 agccatctgg cgcaacagtg ccttgcgact gagagcggcg ggtagatcgg ccaccagggc
     3961 gcgcacctcc gagcgccgcg ccaacgcatc ggcgcgcaac tcgtcgatcg cggcttcggc
     4021 gctctcgacc cggcggcgca gatcggcatt ctcggtgcgg agacggtcga gttcggctgc
     4081 ggcggactcg tcgctcgctc gcgccggctt gttcgaagcg accttgttcg gcgcggcctt
     4141 gttcggcgcg gccttgcgcg gggaggcgct gttcgacgtt gttgggttcg acgtgggctt
     4201 gcccggggtg gtgcctgtca tgacgatgcg gaaccttgga tcgggaggcg gctcaggatg
     4261 gagacggtct cgttgtgggt gaacactcgt cctaccgggc gggggaaggg gaacgagcgg
     4321 atcgagtcga cctgcagcgc ggccatcacg gccagacggc gcacggctgg ctcgtcgaac
     4381 atcgtgacgt gggtggcatc gctggcctga ccgcgcggtt gcggcgtgat gaccagcacc
     4441 cgcccgccgt cgcgcaggaa gggcagatac tgccgaacga gggccaccgc gtcggtctcg
     4501 gtgaggtgtt ccagcacgtg cgcgaacaac agcgagtcga atccggccgg gcgagcctcg
     4561 gcgctggtcg cgaactcgtc gggggtgaac gcgatcagac cgcgggcgcg acacgcggcc
     4621 acgcagtcag ggttgtggtc gacgccgacg ccgttgccgt cgaggtggcc caggttgcgc
     4681 ccgatgccgc acccgatgtc gagcacccgg ccgggctgnn nnnnnnnnnn nnnnnnnnnc
     4741 ggtacgggcg ctgcgggtcg aggacccggc gcaacgtacc gccgcggctc gacagacggg
     4801 tcaggcgctc ggcgtagtcg cgcccctggg tcgacacggt ggtcccggat cgatcgtgcg
     4861 cagtcccggc catcgacgaa agcctacgac gccgccacgc gccatactcg gagccgatgc
     4921 cctccacatc gccctcctcg ctctcgacac cgccagggtc actggaggaa ccgctcgtca
     4981 cggttgccat cccctactac gccaacctgg cctacctcgc cgaggcgttg gccagtgtgc
     5041 aggcacagag cgaccagcgg tggcgggcgc tggtcgtcga cgacgccggc cccgaaccgg
     5101 cgaccgatct ggtggccgcg ctggacgacg cgcgcatcgg tgtggtccgc aacgcggtca
     5161 acctcggtct ggccggcaac tggaaccgtt gcatcgctct ggcgacgacg ccgtgggtga
     5221 cgttgctgca cgccgacgac cggctgcatc gcgactacgt gggtgcggtg ctcgcggcga
     5281 tcgagcaaca tcccgacgtg acggcagtgt tcaccgacgc cgctgtgatc ggcgactacg
     5341 gccagccgcg gcggtcgttg cccgagttcg tcaagcgctt tgcccgccgc cccggcgtcg
     5401 atcatcgcgt cgagggtgat cgcagcttgg cctcgattct gtccaacaac tacgtgatgt
     5461 gccccacgtt gacctactgc accgagccag cccgagcgtt gctgttcgac gaacgctggt
     5521 cgatggtgat ggacctcgac cacaccgcgt cgatgttgct cgacgggcag gcgatgtggg
     5581 cgatccgccg cccgctgtac gagtaccgcc gccacgcggc caacaccacc aacannnnnn
     5641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5701 nggctgggtg gttccgttca gcagccgccg cccgccaccg cgtcgccgtc cgcgccaaca
     5761 ccgcaatcca cggcacagcc gacctcctca gcggccgcgg cctcaagaag ctccgcctcc
     5821 tcgggtccga cctcagttgg tggtgaagta gccgaaggtg tcgacgacga ggttggtggt
     5881 gccggagaag ttgaacaggc gcagctcgcc gttgctcccg acgcggaggt aggcctggtt
     5941 cggcaccggc acgctgggcc ggggattgag cgtcgaagcc accggccgat cggcgccctg
     6001 gggccagaca gtgacgtacg actgcaccga cgcgtccacc gaggtgacgt tggtgacgac
     6061 cgcggaggca tgggccggga tcggtgaggt ggtcacccgc agtgcacggc tctcccccgc
     6121 cgcgaccggc gacgactcac gcgtgtcgag ctgacgggtc gggctgaccg gcacgaacaa
     6181 gccaccctgg tcggagaagt agcccaccac gtccgcgatc acgtgggtgt gcccggcgga
     6241 gttgaagatg ctgatcttgc cgttggcacc caccttggcg atgaccatgt tcgcgatcgt
     6301 ctgacctggc gtgaagttgt gggtcgaggc ggtcggacgc gtctcgccgc tgggccacac
     6361 cgtgagaaat ccgctggacg atgggacgtc gacggtgacg ttgagcgcca cagcttgcac
     6421 gcccctcgcg ggcacgcccc cgcggccgac gacagcgaga tcgatcgcgc tcgcttggct
     6481 gatcggcccc gcagctccca cgcccgttcc atcgcgcgtg tcgagcagcc gagcgggatt
     6541 gagcgcgctg aaccgacctc cggtgacgtt gggtgccgtt gtgtagtagc cgacgatgtc
     6601 ggcgacgaga tgtgtattgc cagccgagtt gaacaggttg acgcgcccgt tggcccccac
     6661 tttgaccgtg accaagttcg gtacggtctg acccggctcg aagttgaggc tcgacaccac
     6721 aggtctcggn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnacgcgt gtcgagcaca
     6901 cgagccggca tcagcggcac gtacgcgctg ggcgccgagg gcaccggccg ttccagacgc
     6961 accggtccgg tcgagtaccc gctgccgaca cccgcagtgt tgcgcatgac catgtacacc
     7021 cagtacgtgc cgttgggcat cgaggagccg tcgaccctgg tgccgttcca ggtgaacgtg
     7081 ttggtaccgg cctgtaccga cacgccgcgg gcgatctggg tgccgttgag gccacgctcg
     7141 tcggtcgtca cgtagatgtc cgccaccgtg cccggggcag cggcctgatc acgaaacgtg
     7201 atcgggtacg acgtcgaaaa cgctgcatcg tctgccagct tgacgttgcg cagggtgaaa
     7261 ccgcgcacgc cccggtcttc gttgagatcg aagcgcaacg cttcgatgtt cacgcctcgc
     7321 caacccgtga cgtacccgct gtcctccagc cgcttggtga cggggctgta gaaccacgcg
     7381 ctgaattggt cgtgtagtcc ggcggcaggg ttggtcacga ggtcgagcga gatctcgtgg
     7441 cagcccggga agatcacgat gtcctgcgtc tcgatccagt tcagcagcgg tgcccctgct
     7501 cggttgtctc tggcacgcca cgcgactctg gccaccatgc ccccgccccg agagtcgtcc
     7561 agnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnat catgggtctc gcccacgtcg
     7741 tccatgcccg cgaagtccca cgggttgccc cgcaccgctg tggcgtaatc ctcaccgccc
     7801 tgctcgttgg gcgtgagcac ctgcgggcgg ggaataccgg acggttgcga gctcggcgca
     7861 tcagcgcgat ggatgcgcac cgaatcgatc tgcaggttga cggctgggtt gcccgcgacg
     7921 agcgcacgaa a
//
LOCUS       DAORNF010000036         7924 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_6750_length_7924_cov_1.789427, whole genome shotgun sequence.
ACCESSION   DAORNF010000036 DAORNF010000000
VERSION     DAORNF010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 7924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7924
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6750_length_7924_cov_1.789427"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="PLV68_02490"
     CDS             <1..294
                     /locus_tag="PLV68_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004512 - inositol-3-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006021 - inositol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HRE00137.1"
                     /translation="PMQIKVNFLCRDSILAAPLALDLVLFSDLAQRAGLYGIQEWLSF
                     YYKSPQTAPGLYPEHDVFIQLTKLKNTLRWIMGEDTINHLGREYYDDMDVDAG"
     assembly_gap    390..409
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<410..950)
                     /locus_tag="PLV68_02495"
     CDS             complement(<410..950)
                     /locus_tag="PLV68_02495"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HRE00138.1"
                     /translation="MPDERADAVPGDGERGGLGGGEDVAATKAALRAEMRRRRAAIDD
                     RSQRVEALWRLVAERLAALPQTAHATVMGFVGVGSEPDTAVLLALLQAAGYRVVLPRV
                     HDGELVAVEHDPAVAMRPGVYGIPEPEGASVDPRLIDVVLVPGLAFTARGERLGQGGG
                     YYDRFLTQLRPGAATWGVGF"
     gene            1043..>1379
                     /locus_tag="PLV68_02500"
     CDS             1043..>1379
                     /locus_tag="PLV68_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00139.1"
                     /translation="MGDEQGIRVGFHFDVMCPWAYQTSKWIRTVRETVPLEIDWRFFS
                     LEEINREDGKKHPWEREWSYGWGMLRVAAMLRRTSMDDCDRFYAAAGEALHEYGRKPH
                     DPAVAAEICA"
     assembly_gap    1380..1389
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1390..1716
                     /locus_tag="PLV68_02505"
     CDS             <1390..1716
                     /locus_tag="PLV68_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00140.1"
                     /translation="GVVQAAIDDPTTHDDVRADHDAVVAHGAFGVPTIVFPNGRHVFG
                     PVVAPAPTGQEALDLWDLTVAYTRVPYLYEIKTPKTDADMTHIATTFQPYLTGRDWES
                     KQNPAP"
     gene            1713..2558
                     /locus_tag="PLV68_02510"
     CDS             1713..2558
                     /locus_tag="PLV68_02510"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HRE00141.1"
                     /translation="MSTSTPTPPLADFVAALNGMFDHYRRLCRDLSDEQLATQSLCPA
                     WSARDVIAHVIGVEAVLDGWEPSLDAMPPFDRLAGYQTDAAALDRDGFTTMIDATIDS
                     RLANLAALGAEHAALLDMPSITPAGPATYGRFLQIRLFDLWVHALDIAVPLGLTAAFP
                     TGFAAERSVDEVQAAMGYIVGKKIGMTEGMSIVFHLTPTATGTSTTAPGVERDIAVAV
                     EGRAHVVDAIATPTTALTMDAQTFVLLAAGRIDPQASIDAGTATWTGDSEWGERAARN
                     LAYTR"
     gene            2603..3109
                     /locus_tag="PLV68_02515"
     CDS             2603..3109
                     /locus_tag="PLV68_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HRE00142.1"
                     /translation="MSDAPGPDFDSAHFRKVLGHFPTGVTIITGMSGDEPAGFTIGSF
                     TSISLEPPLVGFFPMLTSDTWAAMSPSGAFCVNILGAHQGDACWRFAKKGVDDRFDDV
                     TWHPGATGAPIIDDAIAWIDCVTESVQAVGDHYFVVGRVVAMSHAEEDPQPLLFYRGK
                     LGGYSLFS"
     gene            3106..3513
                     /locus_tag="PLV68_02520"
     CDS             3106..3513
                     /locus_tag="PLV68_02520"
                     /inference="COORDINATES: protein motif:HMM:NF022700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3054 domain-containing protein"
                     /protein_id="HRE00143.1"
                     /translation="MKPVDGAAPSFAQRLATPPVAAGVDVVAVLLFVAIGRRSHDEDG
                     SVLSGVFTTAAPLLIALAIGWLIARAWTQPVSLRTGTIVWVATVALGMVLRKLVFDRG
                     TATAFVIVTTIMLCLFLQGWRLGARHLPQRRTA"
     gene            3577..>3875
                     /locus_tag="PLV68_02525"
     CDS             3577..>3875
                     /locus_tag="PLV68_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00144.1"
                     /translation="MTHVLDGVPDDNTTLTAVLDAYAAAGFAASFGVLPGGVVRCDSC
                     ATQLPASSLETASLRRMEGASDPDDMLAVVAMECPACQSRGVLVLGYGPASSAEDG"
     assembly_gap    3876..3896
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4029..>4715
                     /locus_tag="PLV68_02530"
     CDS             4029..>4715
                     /locus_tag="PLV68_02530"
                     /inference="COORDINATES: protein motif:HMM:NF014919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00145.1"
                     /translation="MRVWPGNPLPLGATFDGVGTNIAVFSEIADAIELCVFDSDGNET
                     RIELPERSGSVWHGYFPDLDAGTEYGFRVHGPWDPANGHRCNPAKLLIDPYARAIEGT
                     PDWGQAMFSYRFGDHDQIDTTDSAASAQRGLVVNPFFDWATDQPLLLPWAETVIYEAH
                     VKGATMRHPGVDEELRGSYAGIAHPIFVDHLKSLGVTTLELLPVHQFVHDSHLGERGL
                     RNYWGYNSIGY"
     assembly_gap    4716..4725
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4726..>5873
                     /locus_tag="PLV68_02535"
     CDS             <4726..>5873
                     /locus_tag="PLV68_02535"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HRE00146.1"
                     /translation="PHHEYSHRHGQGQQVQDFKQMVKTLHQAGIEVILDVVYNHTAEG
                     NHLGPTLSFKGIDNASYYRLVGDDKQYYMDYTGTGNTLNMQNPFVLQLVMDSLRYWAT
                     EMHVDGFRFDLASTLARSLHEVDKLSAFFDLVQQVPVISRMKLIAEPWDVGEGGYQVG
                     NFPPQWAEWNGRYRDCIRDHWIGGTHGLGEFAYRITGSSDLYQADGRKPHASINFVTA
                     HDGFTLRDLVSYNDKHNEANGEDNNDGESHNRSWNCGAEGPTEDPGINALRARQQRNL
                     IATLLLSQGVPMLLGGDELGRTQGGNNNAYCQDNEVSWFDWDHVDAGLLAFTTGVIAM
                     RRDHPTFRRRQFFQGHSLHGEGTVDLAWFDSDGTEMTDDQWGDDHRYV"
     assembly_gap    5874..5951
                     /estimated_length=78
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5952..6156
                     /locus_tag="PLV68_02540"
     CDS             <5952..6156
                     /locus_tag="PLV68_02540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00147.1"
                     /translation="AGHDPITVTLPDARWGDGWRTVVCTASGAIDTPDAPVQPPGATL
                     DLDSFSLLLMEVVPDESTMETAD"
     gene            complement(6183..7742)
                     /locus_tag="PLV68_02545"
     CDS             complement(6183..7742)
                     /locus_tag="PLV68_02545"
                     /inference="COORDINATES: protein motif:HMM:TIGR02777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase ligase N-terminal
                     domain-containing protein"
                     /protein_id="HRE00148.1"
                     /translation="MATYQSMRDFDATPEPAGQVFVNHTGVRRFVVQRHRATRLHYDV
                     RLEVDGVLVSWAVPRGPSLDPARKSLAVKVEDHPYDYGWFEGNIPSGYGKGDVIIWDD
                     GWWEPDPAYPDSADPAAAIAAGELKLVLHGHKVRGRYVLVRTGGKNRDDDSQWLMIHK
                     KDDDAVPGWDPEDHPRSVLSARTNVDVVEGRAGRWPAATADELDALANMGPKGEWSVG
                     GQPVALTNLDKVMMPGRPGAAGQRGAPVTKRDIVAYYTRIAPWLSPYLAGRPINLNRF
                     PDGIDAAKRGFWQKAVPAHAPDFVRRWPNPLADDGKTRDYLLIDGAASLAWVANHAGF
                     EIHPWTSTAAAPQQPSYALVDIDPGPATTWDETLTLARLFHMATDNLGLVARAKVTGK
                     RGIQIWIPVATGYTFRETSDFVEALSRTVGRVVPDLVSWQWAKSERNGKARLDYTQNA
                     INKTLVAPYSVRPAAGAPVSVPITWDELDDPDLAPDRWTIHDIFARLDTIGDPFLALH
                     AVTQSLPPLAG"
BASE COUNT         1246 a         2752 c         2545 g         1242 t
ORIGIN      
        1 ccgatgcaga tcaaggtcaa cttcctgtgc cgcgacagca ttctcgccgc acctctggcg
       61 ctcgacctgg tgttgttcag cgacctggcc cagcgcgccg ggctgtacgg cattcaggag
      121 tggctgagct tctactacaa gagcccccag accgcccccg gcctgtaccc cgagcacgac
      181 gtgttcatcc aactcaccaa gctgaagaac acgctgcgct ggatcatggg cgaggacacc
      241 atcaaccact tgggccgcga gtactacgac gacatggacg tcgacgccgg ctgacctcgc
      301 gtcgtcgggt tctgcgcttc ctaccgtcgc ggcgatctac gcgtcgatct acgcgacgat
      361 caccgcatcg agcaccacgt cgtgggcctn nnnnnnnnnn nnnnnnnnng gaacccgacc
      421 ccccacgttg ccgcgcccgg ccgcagttgg gtgaggaagc ggtcgtagta cccgccccct
      481 tgaccgagcc gctcgccgcg agcggtgaag gccagtcccg gtacgagcac gacgtcgatc
      541 aggcgcggat cgacggacgc gccctctggc tccgggatcc cgtacacgcc gggacgcatc
      601 gccaccgcag gatcgtgttc gacagcgacc agctcgccgt catgcacacg cggcagtacc
      661 acgcggtacc cagccgcctg caggagggcg agcagcacgg ccgtgtcggg ctcgctcccc
      721 acccccacaa accccatcac cgttgcgtgt gcagtctggg gcagcgccgc gagccgctcg
      781 gccaccagcc gccacaacgc ctcgacccgc tgagaacgat catcgatcgc ggcccgccgc
      841 ctccgcatct ccgcccgcaa cgcggccttc gtcgcagcaa cgtcctctcc gccaccgagc
      901 ccgccgcgtt cgccatcccc cggcacagcg tcggcgcgct cgtctggcat gactccaccc
      961 tgccacgtgg ggcgtgttcg attcacctcg actccggggg ccacgtgaac acacctcggc
     1021 ggctgggtgt acggtcgtcg cgatggggga tgagcagggc atacgggtgg ggtttcactt
     1081 cgatgtgatg tgcccgtggg cgtatcagac gtcgaagtgg attcgcacgg tccgcgagac
     1141 ggtgccgctg gagatcgact ggcggttctt ctcgctggag gagatcaacc gcgaggacgg
     1201 caagaagcac ccgtgggagc gggagtggtc gtacggctgg ggcatgctgc gcgtcgcggc
     1261 gatgctgcgg cgcacgtcga tggacgactg cgaccggttc tacgccgcgg ccggcgaggc
     1321 gctgcacgag tacggccgca agccgcatga cccggcggtg gcggccgaga tatgcgcccn
     1381 nnnnnnnnng gcgtggtgca agccgcgatc gacgacccga ccacccacga cgacgtgcgg
     1441 gccgatcacg atgcggtcgt ggcccacggc gcgttcgggg tgccgaccat cgtcttcccc
     1501 aacggacgcc acgtgttcgg cccggtcgtc gcacccgcgc ccaccggtca ggaggcgctc
     1561 gacctgtggg acctcaccgt cgcgtacacc cgggtgccct acctctacga gatcaagaca
     1621 cccaagaccg atgccgacat gacccacatc gccaccacct tccagccgta cctcaccggc
     1681 cgcgactggg agagcaagca gaaccccgcg ccatgagcac atcgacgcca acaccgccgc
     1741 tggccgactt cgtcgccgcc ctcaacggca tgttcgacca ttaccgtcgg ctgtgccgcg
     1801 acctgtcgga cgagcaactc gccacccagt cgctgtgccc ggcatggagc gcccgcgacg
     1861 tgatcgccca tgtgatcggg gtcgaagccg tgctcgacgg atgggagccg tcgctcgacg
     1921 ccatgccacc gttcgaccgg ttggccggct accagaccga cgcggccgcg ctcgaccgtg
     1981 acggcttcac cacgatgatc gatgccacga tcgactcccg cctggccaac ctggctgcgc
     2041 tcggcgccga gcacgcggca ctgctcgaca tgccgtcgat cactccggcc ggtcccgcca
     2101 cctacggccg cttcctgcag atccgcttgt tcgacctgtg ggtccacgcg ctcgacatcg
     2161 cagtgccgct gggcctcacc gctgcgttcc cgaccggctt cgctgccgag cgatcggtcg
     2221 acgaagtgca agccgcaatg ggctacatcg tcggcaagaa gatcggcatg accgagggca
     2281 tgtcgatcgt gttccacctg acgccaacgg ccacaggtac gagcacgacc gcaccgggtg
     2341 tcgagcgcga catcgcggtg gcggtcgagg gccgcgccca cgtggtcgac gcgatcgcca
     2401 cgccgaccac cgcgctcacg atggacgcgc aaaccttcgt gttgctcgcc gcgggacgga
     2461 tcgatccgca ggcgtcgatc gacgccggga ccgcgacctg gaccggcgac agcgagtggg
     2521 gcgagcgcgc cgcgcgcaac cttgcctata cccgctgacc agtctggctc tggcgggtcg
     2581 gaggccgtta gtgtcggccg cgatgtctga cgcgcctggc cccgatttcg attccgccca
     2641 cttccgcaag gtgctggggc actttcccac cggtgtcacg atcatcaccg gcatgagcgg
     2701 tgacgaaccg gccgggttca cgatcggctc gttcacgtcg atctcgctcg aaccgccgct
     2761 ggtcggcttc ttcccgatgc tcaccagcga cacctgggcg gcgatgtcgc cgtccggcgc
     2821 gttctgcgtc aacatcctcg gcgcccacca gggtgatgcc tgctggcgct ttgccaaaaa
     2881 aggcgtcgac gatcgcttcg acgacgtcac ctggcatccc ggcgccaccg gcgccccgat
     2941 catcgacgac gcgatcgcgt ggatcgactg cgtgaccgag tcggtgcaag cggtcggcga
     3001 ccactacttc gtggtcggca gggtggtggc gatgagccat gccgaggaag acccgcagcc
     3061 gctgttgttc taccgcggca agctcggcgg ctactccctg ttctcatgaa gcccgttgac
     3121 ggcgccgctc cgtcgttcgc acagcgcctc gccactccgc ccgtcgcggc gggggtcgac
     3181 gtcgtcgccg tgctgttgtt cgtcgcgatc ggtcggcgca gccacgacga ggatggcagc
     3241 gtgctcagcg gtgtgttcac cactgcggca ccgctgctga tcgccctcgc gatcggctgg
     3301 ttgatcgccc gcgcctggac ccagccagtc tcgttgcgca ccggcacgat cgtgtgggtg
     3361 gccacggtcg cgctgggcat ggtgctgcgc aaactggtct tcgaccgcgg tacggcgacc
     3421 gcgttcgtca tcgtgaccac gatcatgctc tgcctgttcc tccagggctg gcgtctgggc
     3481 gcgcgccacc taccgcagcg ccgcacagcc tgacgcagcg accagcccgc cggcgcatgg
     3541 tgtcgcccgg cgccggtttg gggtacaact ggggccatga ctcacgtgct cgacggcgtc
     3601 ccggacgaca acacgaccct gaccgcggtg ctcgacgcgt acgccgcggc cggcttcgcc
     3661 gcgtcgttcg gggtcctccc cggcggcgtg gtccgctgcg attcgtgcgc cacccagttg
     3721 ccggcctcgt cgctcgagac cgcatcgctg cggcgcatgg aaggagcgtc cgatccggac
     3781 gacatgctcg cggtggtcgc gatggagtgt ccggcgtgtc agtcgagagg cgtgctggtg
     3841 ctgggctatg gtcctgcgtc gagcgcggaa gacggnnnnn nnnnnnnnnn nnnnnncatc
     3901 gctgtggtcc cccgctggcc ccgcattccg cgccgggtga agccgtcggc gacacctgcg
     3961 actgaggttg cccgagcctc ccatcgcgtc cgcaacaccg cgctcgctac gcgatcgaaa
     4021 ggcacccatt gagagtttgg ccaggcaacc cgcttcccct cggcgccacc ttcgacggcg
     4081 tcggcaccaa catcgcggtg ttctccgaga ttgccgacgc gatcgaactg tgtgtgttcg
     4141 acagcgacgg caacgagaca cggatcgagt tgcccgagcg gtcgggttcg gtgtggcacg
     4201 gatactttcc tgacctcgac gcaggcaccg agtacgggtt ccgggtgcac ggcccgtggg
     4261 atccggccaa cggtcaccgc tgcaacccgg ccaagttgct gatcgacccg tacgcccggg
     4321 cgatcgaggg cacccccgac tggggccagg cgatgttctc gtatcgcttc ggcgatcacg
     4381 accagatcga caccaccgac agcgccgcgt cagcccagcg gggcctggtg gtcaacccgt
     4441 tcttcgactg ggccaccgac cagccgctgc tgttgccgtg ggccgagacg gtgatctacg
     4501 aggcccacgt caagggcgcc acgatgcgcc atcccggcgt ggacgaggag ctgcgcggca
     4561 gctacgccgg catcgcccac ccgatcttcg tcgatcacct caagtcgctc ggcgtgacca
     4621 cgttggagct gttgcccgtc catcagttcg tccacgacag ccacttgggc gagcgcgggt
     4681 tgcgcaacta ctgggggtac aactcgatag gctacnnnnn nnnnngccgc accacgagta
     4741 ctcgcaccgg cacggccagg gccaacaggt gcaggacttc aaacagatgg tcaagacgct
     4801 gcaccaggcc ggcatcgagg tgatcctcga cgtggtctac aaccacaccg ccgagggcaa
     4861 ccacctcggc ccgacgctgt cgttcaaggg catcgacaac gcgtcgtact accgcctggt
     4921 cggcgacgac aagcagtact acatggatta caccggcacc gggaacacgc tcaacatgca
     4981 gaacccgttc gtgctgcaac tggtgatgga cagcctgcgc tactgggcca ccgagatgca
     5041 cgtcgacggg ttccgattcg acctcgcctc cacgctggcg cggagtctgc acgaggtcga
     5101 caagctgtcg gcgttcttcg atctggtgca acaagtcccg gtgatcagcc ggatgaaact
     5161 gatcgccgaa ccgtgggacg tcggcgaggg cggctaccag gtcggcaact tcccgccaca
     5221 gtgggcggag tggaacggca ggtaccgcga ctgcatccgc gaccattgga tcggcggcac
     5281 ccacggattg ggcgagttcg cctaccgcat caccggttcg agcgacctgt accaagccga
     5341 cggtcgcaag ccccatgcga gcatcaactt cgtcaccgcc cacgacgggt tcaccctgcg
     5401 cgatctcgtc agctacaacg acaagcacaa cgaagccaac ggcgaggaca acaacgacgg
     5461 cgagagccac aaccggagct ggaactgtgg cgccgaaggc ccgaccgagg accccggcat
     5521 caacgccctg cgcgcccgcc aacagcgcaa cctgatcgcg accttgctgt tgtcccaggg
     5581 tgtgccgatg ctgctcggcg gtgacgagtt gggccgcacc caaggcggca acaacaacgc
     5641 ctactgccaa gacaacgagg tctcgtggtt cgattgggac cacgtcgacg ccgggctgct
     5701 cgcgttcacc accggcgtga tcgcgatgcg ccgcgatcat cccacgttcc gtcgtcgtca
     5761 gttcttccaa ggccattccc tacacggcga aggcaccgtc gatttggcct ggttcgactc
     5821 ggacggcacc gagatgaccg acgaccagtg gggcgacgac catcgctacg tcgnnnnnnn
     5881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5941 nnnnnnnnnn ncgccggtca cgacccgatc acggtcaccc tgccggacgc gcgctggggc
     6001 gacggatggc gcacggtggt gtgcaccgcg tccggcgcga tcgacacgcc cgacgccccc
     6061 gtccagccgc caggagcgac gctcgacctg gacagcttct cgctgctgct gatggaggtt
     6121 gtgcccgacg agtccacgat ggagacagcc gactgacccg ccccgtcgtc ggggccagcg
     6181 cgtcaaccag ccaagggcgg caacgactgg gtgacggcgt gcaatgccag gaacgggtcg
     6241 ccgatggtgt cgagccgggc gaaaatgtcg tggatcgtcc accggtcggg ggccaggtca
     6301 gggtcgtcga gctcgtccca cgtgatcggc accgacaccg gtgcccccgc agcggggcgc
     6361 acgctgtacg gcgcgaccag cgtcttgttg atcgcgttct gggtgtagtc gagccgggcc
     6421 ttgccgttgc gctcgctctt ggcccactgc cagctgacca ggtcgggcac cacccgcccg
     6481 accgtgcgcg acaacgcctc gacgaagtcg gacgtctcgc ggaacgtgta gccggtggcg
     6541 acggggatcc agatctggat gccacgcttg ccggtgacct tggcccgcgc gaccaacccc
     6601 aggttgtcgg tggccatatg gaacagccgg gccaacgtca gcgtctcgtc ccaggtcgtg
     6661 gcggggccgg gatcgatgtc gaccagcgcg tacgacggtt gctgtggggc ggccgcggtc
     6721 gacgtccacg gatggatctc gaaaccggcg tggttggcca cccacgccag cgatgcggcg
     6781 ccgtcgatca acaggtagtc gcgggtcttg ccgtcgtcgg ccagcgggtt cggccagcga
     6841 cgcacgaagt cgggcgcgtg agccggcacc gccttctgcc agaacccccg cttggcggcg
     6901 tcgatgccgt cgggaaagcg gttgaggttg atcggccggc cggccagata cggcgacaac
     6961 cacggggcga tgcgggtgta gtaggcgacg atgtcgcgct tggtgaccgg cgctccacgt
     7021 tgcccagcgg cgccggggcg gccgggcatc atcaccttgt cgaggttggt cagcgcgacc
     7081 ggctgaccac cgaccgacca ctcccccttc ggtcccatgt tcgccaacgc gtccagttcg
     7141 tcggcggtgg cagcgggcca acggccagcg cggccttcga ccacgtcgac gttggtgcgc
     7201 gcgctcaaca ccgaccgtgg atggtcttcg gggtcccagc ccggcacggc gtcgtcgtcc
     7261 ttcttgtgga tcatcagcca ctgggagtcg tcgtccctgt tcttgccacc cgtgcgcacc
     7321 agcacgtaac ggcccctgac cttgtggccg tggagcacca gcttcagctc accggcggcg
     7381 atcgcggccg ccgggtcggc gctgtcggga taggccgggt cgggctccca ccaaccgtcg
     7441 tcccagatga tcacgtcacc cttgccgtag ccactgggga tgttgccctc gaaccacccg
     7501 tagtcgtacg ggtgatcttc gaccttgacc gcgagcgact tgcgggccgg gtcgagcgaa
     7561 ggtcctcggg gcacggccca actcaccagc acgccgtcga cctcgaggcg gacgtcgtag
     7621 tgcaaccgcg tcgcccgatg acgctggacg acgaagcgac ggaccccggt gtggttgacg
     7681 aacacctgcc cggcgggctc gggtgtcgcg tcgaagtcgc gcatcgactg gtatgtcgcc
     7741 aacggaggct catgccggct cacccgaaca tcatgcccga ccgccagatc aggcgctctt
     7801 gcgcaccggc gccttcgacg cggctggctt cttggccgcc ttggtggcct gtgccgccgc
     7861 gggcttcgcg gccgccttct tggcaggctt ggcttcggcc tcgggcgtgg ccggggtctg
     7921 gttg
//
LOCUS       DAORNF010000037         7903 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_6770_length_7903_cov_1.850917, whole genome shotgun sequence.
ACCESSION   DAORNF010000037 DAORNF010000000
VERSION     DAORNF010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 7903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7903
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6770_length_7903_cov_1.850917"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /locus_tag="PLV68_02550"
     CDS             complement(<1..352)
                     /locus_tag="PLV68_02550"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRE00149.1"
                     /translation="MRALFASPLGFLIGLSLGALGGGGSILAVPALVYGAGQGPKAAT
                     TTSLLLVGIASLVGMVTHWRNGRVRLGTGIVFGLVGIGGSLAGSALNKQLNPDLLLLG
                     FAGLTCIAAWRMLTG"
     gene            complement(721..>1086)
                     /locus_tag="PLV68_02555"
     CDS             complement(721..>1086)
                     /locus_tag="PLV68_02555"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRE00150.1"
                     /translation="VLAAHPELATPATRLTAADVRNWLDDDPTLQIVDVRNPGETRIG
                     GTVPGATTVPLPQLLEHLHELDPERPVIVYCAGGYRSSIAASTLRAHGFTTVADIIGG
                     YGAWTTLNTTPPTPENTDV"
     assembly_gap    1087..1255
                     /estimated_length=169
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1256..>1951)
                     /locus_tag="PLV68_02560"
     CDS             complement(<1256..>1951)
                     /locus_tag="PLV68_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRE00151.1"
                     /translation="VIETHFHADFLSGHLELAAATGAVISYGSAATPDFEIDPLTDGQ
                     VLSLGDVALEIRHTPGHTPESISIVVSEHAGTGPWAVLTGDTLFIGDVGRPDLLTSVG
                     RTADDLARNLFRSIHSQLLTLPDHTLVYPAHGAGSACGKHMSAAASSTIGEQRATNYA
                     LAPMTEDQFVAAVTEGQSVAPLYFAFAADANRRERDLLDDQQPPPTLDVADLAKLGDD
                     IVGIVNGKSSLGR"
     assembly_gap    1952..1979
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2110..>2872)
                     /locus_tag="PLV68_02565"
     CDS             complement(2110..>2872)
                     /locus_tag="PLV68_02565"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HRE00152.1"
                     /translation="VTDVAANGRTVVRITPSTTVDSTATADLVERLRDRLDSVAPEAR
                     LGGPGTQNTDLTSALLDSAPLAIAAIMLVAFVLLLVVFRSWLIALTSIVFNLISVAAS
                     FGFATLVFQHGYGAGVIGVEHQGFVDAWAPLFFFALLFGLSMDYQLFLLAAIKERRDA
                     TGDARLAVREGIARTGRPITNAALIMIVVFIAFGVTGPIPPTELGITLALAVLLDATV
                     IRMMLVPALLGMLGERAWNLPGWLDRRLPHIDFAH"
     assembly_gap    2873..2888
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2889..>3241)
                     /locus_tag="PLV68_02570"
     CDS             complement(<2889..>3241)
                     /locus_tag="PLV68_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00153.1"
                     /translation="TLLPAVLVAMGDRVLVARGRNNADRAAEGRWTRWTSKAMQHPGR
                     TLAAGLGDLLLLAAPALGMRLGMPAAGVVDQGNQSRDGYDAVVANFGPGAPAPMFATV
                     PVAQADAVVAAALLD"
     assembly_gap    3242..3273
                     /estimated_length=32
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3274..4142)
                     /locus_tag="PLV68_02575"
     CDS             complement(<3274..4142)
                     /locus_tag="PLV68_02575"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HRE00154.1"
                     /translation="MVAVIAAAPLAATLGSALSGAGWDAQGSTAADVRAEIRRDFPDL
                     GAEAAIVAYRQPSPIADDPAGLAALVAALQGSPGADRVVDPLTQPADAGLISADGRAA
                     LVPVALSANEDADLPESAGQVMQTVASLELPPGAQADTTGEWAVWHDFNQTNEQALHK
                     AELLSGLPTIILLFIAFGSAIAAGIPLILAVSGIAVGFAALHLGTTLLPLSVWSMNFS
                     MMIGLAVGIDYGLFIVSRYREERADGKDAVDGLTATLATAGKAVFLSALTVVLSLAAI
                     FIVPIMVFRSMALG"
     gene            complement(4347..4535)
                     /locus_tag="PLV68_02580"
     CDS             complement(4347..4535)
                     /locus_tag="PLV68_02580"
                     /inference="COORDINATES: protein motif:HMM:NF014624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HRE00155.1"
                     /translation="MLTDGRDCRDVVTQISAASKALDQAGFVLVAAGLTWCLEDPERS
                     KAQGYDVGDVQKMFTKLA"
     gene            complement(5001..5399)
                     /locus_tag="PLV68_02585"
     CDS             complement(5001..5399)
                     /locus_tag="PLV68_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRE00156.1"
                     /translation="MTSRTFLGELACVAAARRFVRDTLQGEGQDAACDAAVLMVSELA
                     TNVVLHAGTSFEIVLDVSNGLIRVEIHDGMAVSHAFRDLIENPPTVVEPVAAGGRGIM
                     LIGLTALRFGLQDNGPEGKAVWFELPAVVS"
     gene            complement(5862..6245)
                     /locus_tag="PLV68_02590"
     CDS             complement(5862..6245)
                     /locus_tag="PLV68_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00157.1"
                     /translation="MNGVTYDTGALIAADRNDRRMWALHSGLLALEVSPTVPAPVLAE
                     AWRGGSRQASLARLLALCTTEPLTEDQAKAVGTLATRAGHDDIVDVTVVEGAIRRHDA
                     VVTSNPTHIRKIADATRARLTIETI"
     assembly_gap    6371..6380
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6720..6875
                     /locus_tag="PLV68_02595"
     CDS             6720..6875
                     /locus_tag="PLV68_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00158.1"
                     /translation="MRVRPPPRALPHHSLSRRVERTPPERLARYVELLQSEMPNTLAI
                     LVPFLAE"
     gene            complement(6886..7296)
                     /locus_tag="PLV68_02600"
     CDS             complement(6886..7296)
                     /locus_tag="PLV68_02600"
                     /inference="COORDINATES: protein motif:HMM:TIGR01764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family DNA-binding protein"
                     /protein_id="HRE00159.1"
                     /translation="MALTTDRRERSEARNAAEIVDAIRADGLADIAVSIGGRNYPLGP
                     ELVALVATLLDDVSYGREVISAPAGLPIGTELASELLGVSRPWLTTLLDRGEIPSTRA
                     GTKRRMRLGDVLAYRRADERRREAVLTWDFLDEA"
     gene            complement(7461..7622)
                     /locus_tag="PLV68_02605"
     CDS             complement(7461..7622)
                     /locus_tag="PLV68_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00160.1"
                     /translation="MSNDAVPVPHPESLEVPVAELLRRARPLPPLGEMVIDDLTDEEA
                     EAFLAAFRS"
BASE COUNT         1264 a         2422 c         2687 g         1275 t
ORIGIN      
        1 agccggtgag catccgccat gcggcgatgc aggtgaggcc ggcgaagccg agcagcaaga
       61 ggtcggggtt gagttgcttg ttgagcgcgg atccggcgag ggagccgccg atcccgacga
      121 gtccgaacac gatgccggtg ccgagacgga ctcgaccgtt gcgccagtgc gtgaccatcc
      181 cgacaagcga cgcgatgccg accaggagga ggctggtggt cgtggcggct ttgggacctt
      241 ggccggctcc gtagacgagg gcggggacag cgaggatcga tccaccacca ccgagggctc
      301 cgagggagag cccgatgagg aagccgagcg gtgacgcgaa cagggcgcgc atgatctact
      361 cgcctcgctt gtcggctcgt tggccgttgt tcgccgtagg cggcgccgag gtgggccagc
      421 acgtcgcgga cgacgggcag gccggctgct gcccatgcgg tcatgccgcc ggtgacgttg
      481 gtggcgttga tgcctgctgc gcgcaggatg gtggtggctc tgttggatcg tccgcccgat
      541 cggcacacgc acatgacgtc gacgccgctt gggatcgagg tggcgttgag ggcactgagc
      601 ggcatgtggg tggcgtcggg cgcgtgtccg gcggcccatt cgtcgggttc gcggacgtcg
      661 agaaggattg cgtcccccgt cctgtgtgcg gcgctggcgg gggtgatttc ggggttggtg
      721 tcagacatct gtgttctccg gtgtcggtgg tgtggtgttg agggtggtcc aggcgccgta
      781 gccgccgatg atgtcggcga cggtggtgaa gccgtgggcg cgcagggtgg acgcggcgat
      841 cgacgagcgg tagccgcctg cgcagtagac gatcactggg cgttcggggt cgagctcgtg
      901 gaggtgttcg aggagttgcg gcagggggac ggtcgttgcg cccgggacgg tgccgccgat
      961 gcgggtttcg cccgggttgc ggacgtcgac gatctgcagg gtggggtcgt cgtcgagcca
     1021 gttgcgcacg tcggcggctg tgagtcgggt tgccggggtg gcgagttcgg ggtgggcggc
     1081 gaggacnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnggcgg
     1261 cccaggctcg acttgccgtt gacgatgccg acgatgtcgt cgccgagctt ggcgaggtcg
     1321 gcgacgtcga gtgtcggtgg cggttgctgg tcgtcgagga ggtctcgttc gcggcggttg
     1381 gcgtcggcgg cgaatgcgaa gtagagcggc gcgacggatt ggccttcggt gaccgcggcg
     1441 acgaactggt cttcggtcat cggggcgagc gcgtagttcg tcgcccgttg ctctccgatt
     1501 gtggacgacg ctgctgcaga catgtgcttg ccgcatgccg atcccgcacc gtgtgccgga
     1561 tagacgagcg tgtggtcggg gagggtgagc agttggctgt ggatggagcg gaacaggttc
     1621 cgggcgaggt cgtcggcggt gcggccaaca gaggtgagca ggtcgggtcg cccgacgtcg
     1681 ccgatgaaca gcgtgtcgcc ggtcagcacg gcccacgggc cggtgccggc gtgttcgctg
     1741 accacgatcg agatcgactc cggggtgtgc ccgggggtgt ggcggatctc aagcgcgacg
     1801 tcaccgagcg acagcacctg tccatcggtg agcgggtcga tctcgaaatc cggagtagcc
     1861 gccgacccgt aggagatgac ggcaccggtc gccgcagcga gctcgagatg gccagacaag
     1921 aagtcggcgt ggaagtgggt ttcgatcaca cnnnnnnnnn nnnnnnnnnn nnnnnnnnnc
     1981 ttgatctgct tctcgacttc ggccgatgcg gcgaccacgg cgcggccggt cgtggtgtcg
     2041 ccgatcatgt agctgtacag cgacaggcag gcgaggtcgt attggcggaa gtacatggcg
     2101 tggctccttt cagtgggcga agtcgatgtg cgggagtcgc cggtcgagcc agccgggcag
     2161 gttccaggcg cgttcgccga gcatcccgag aagcgctggg acgagcatca tccggatcac
     2221 ggtggcgtcg aggaggacgg cgagggcgag cgtgataccg agctcggtcg gcgggatcgg
     2281 ccccgtaaca ccgaacgcga tgaacaccac gatcatgatc agcgcagcgt tggtgatcgg
     2341 tcggccggtg cgggcgatgc cttctcgcac ggcgaggcgc gcgtcaccag tggcatcgcg
     2401 tcgttccttg atcgcagcga ggaggaaaag ctggtagtcc atcgacaggc cgaacagcag
     2461 cgcgaagaag aacagcggtg cccaggcgtc gacgaaacct tggtgttcga cgccgatcac
     2521 tccggcgccg tagccgtgtt ggaagaccag cgtcgcgaac ccgaagctgg cggcgacgct
     2581 gatcaggttg aacacgatcg aggtcagtgc gatcagccag cttcggaaca cgacgagcag
     2641 caggacgaac gcgacgagca tgatcgcggc gatggcgagc ggggcgctgt ccagcagggc
     2701 gctggtgagg tcggtgttct gtgttcccgg accgcccagc ctcgcttcgg gcgcaacgga
     2761 gtcgagtcgg tcgcggagac gttcgacgag atctgcggtt gcggtcgagt cgaccgtcgt
     2821 tgacggcgtg atgcgcacca cggtgcggcc atttgcggcg acgtcggtga cgnnnnnnnn
     2881 nnnnnnnnga tcgaggagcg ccgcggcgac cacggcgtcg gcttgggcga cgggcacggt
     2941 ggcgaacatc ggggctggtg cgcccgggcc gaagttggca acgacggcgt cgtagccgtc
     3001 gcggctctgg ttgccctggt cgacgacacc cgccgctggc atgccgagcc gcattccgag
     3061 cgccggtgct gcgagcaaca ggaggtcgcc gaggccggcg gcgagcgtcc ggccgggatg
     3121 ctgcatcgcc ttcgacgtcc atcgtgtcca gcggccctcc gcggcgcggt cggcgttgtt
     3181 gcggccgcgt gcgacgagca cccggtcacc catcgcgacc agcacggcgg ggagcagtgt
     3241 gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncccaacg ccatcgaccg gaagaccatg
     3301 atcggcacga tgaagatcgc ggcgagggac aacacgaccg tcagggcgga caggaagacg
     3361 gctttgcctg cggtcgccag tgtcgcggtt aggccgtcga cggcgtcctt gccgtcggcg
     3421 cgctcttccc ggtagcggga gacgatgaac aacccgtagt cgattccgac cgcgaggccg
     3481 atcatcatcg agaagttcat cgaccacacc gacagcggca ggagcgtcgt gccgaggtgc
     3541 agggcggcga aaccgacggc gatgcctgac accgcgagga tcagtgggat cccggcggcg
     3601 atcgccgagc cgaacgcaat gaacagcaag atgatcgtcg gcaacccaga cagcagttcc
     3661 gccttgtgca gcgcctgttc gttggtctgg ttgaagtcgt gccacacggc ccactcgccg
     3721 gtggtgtcag cctgggcacc tggcggcagc tccaacgatg ccacggtctg catgacctga
     3781 ccggcggact cgggcagatc cgcatcctcg ttcgctgaca gcgcaaccgg caccagcgcc
     3841 gcgcgtccgt ccgcgctgat cagcccggca tcggcaggct gcgtcaacgg gtcgacgacc
     3901 cggtccgcgc cagggctgcc ctgcaatgct gcaaccaggg cggcgagacc agcagggtca
     3961 tccgcgatcg gcgacggctg gcggtaggcg acgatcgcgg cctccgcgcc gaggtcgggg
     4021 aagtcacggc ggatctcggc gcgcacgtcc gcggcggtcg aaccctgcgc gtcccagccg
     4081 gccccggaca aggccgatcc caatgtggca gcgagcggcg cagcggcgat caccgcaacc
     4141 acccatgtca cgatcactct gcggggatgg cgtgcactgg accgaccgat tcggcccaac
     4201 acgccgaggc gtgtcggcgt cggcgtcggc gtcggtgtga gcggcggggt caacaacgtt
     4261 ttcatcggag tctctcggtg tttcggggca ggcgcagacc ggcggctgcc ggggtatcgg
     4321 tatcggtcga accgcttgcg atgcggtcag gccagcttcg tgaacatctt ctggacgtcg
     4381 ccgacgtcgt acccctgagc cttcgaccgc tccgggtcct ccaggcacca cgtcaacccg
     4441 gcggccacca acacgaaacc ggcctgatcg agcgccttgc tcgcagcaga gatctgcgtg
     4501 accacatcac ggcaatcgcg cccgtcagtc agcatctgct gcacaccgcg cacctgccac
     4561 gaagtcgctt gatcagatcg tcaatggtcg cttccggaag ttccatggat ctcaacatac
     4621 ccctacgggt actggtacac aacatacctc cgggggtacc tacaggcagg tcgtcgcgcg
     4681 cccgacgtcg ttcgaggctg acggcctgcg cgtcggtgct cacctccgta ttcagcggcc
     4741 tcggcggcgg cgcgatgggt cgctgcaggt tggcggacga tggtcgggtt catgcgacgg
     4801 ctcgacgccg gtatcgccca cagcggaatc aggtcgcgct tgcctgccgc cccagccggg
     4861 cgaagcagcg acggtcatca agcgccgtcc gcagtagcga tcaatccacc gggatgtctg
     4921 ctcaacatgg ttcgtcgacc cgccttatcg ctcgacgcgc cagccatgct gcggcttgcc
     4981 aagagcacgg agttgtgcgg ctaactcact accgcaggca actcaaacca cacggccttt
     5041 ccttccgggc cgttgtcctg gagcccgaac cggagagctg tcaaaccaat cagcatgatc
     5101 cctcgtccgc cggcggctac tggctcaacc accgtcggcg ggttctcgat gagatcccgg
     5161 aacgcgtgac tgacggccat cccatcgtga atctccacac gaatcaaccc attgctgaca
     5221 tcgagcacga tctcgaacga ggtcccggcg tggagcacga cattcgttgc gagctcgctc
     5281 accatcaaga cagcagcatc acacgcagca tcctgtcctt cgccttgcaa ggtgtcgcga
     5341 acaaatcggc gcgcggccgc aacgcacgcc aactcaccca gaaacgtcct cgaagtcacc
     5401 gaccgtcccc ttgcatcacc agcaggcctc gaccctgtcc cggtcagcgc ttctcctgaa
     5461 acactgcgct acagccgtgg gcggtgcgca tcgatccgcc aagcgcccgt ctggttgtga
     5521 aagcctcccg acgttcacca gcgtttttct ccacgagatg ctgaggagtg tcgccgtcgc
     5581 tgcgcttgtc ctcatcatcg aaggaacggg tcgactctga acgtgacccg gattcctgga
     5641 cgcggggtga cgtgagatcg aggccttcat ggccagcctc ggcgccacgc gtcagcgtgc
     5701 aacttcctcg ccgtgcttcc gctggcccgt ctcgcctcgg cggccttcgt ggtggtgctc
     5761 gttgtcgacg tcgtcacgtg gacatcgaat cgtgaacccc tccacgaaga ggcgatcgac
     5821 cggggtgaca actacgctgc acgcatcggg ttgccacggt gtcagatggt ctcgatggtg
     5881 agcctggctc gtgtggcgtc ggcgatcttg cggatgtggg tcgggttcga tgtcaccacg
     5941 gcgtcgtgtc ggcggatagc tccttcgacg acggtgacgt cgacgatgtc gtcgtgcccg
     6001 gcgcgggtcg ccagcgtgcc gacggccttc gcttggtctt cggtcaatgg ttcggtggtg
     6061 cacagagcca ggagccgggc gaggctcgcc tggcgcgacc cgccgcgcca cgcttcggcc
     6121 agaactgggg ccggcacggt cggggacacc tcgagcgcga ggaggccaga gtggagcgcc
     6181 cacatccgtc ggtcgttgcg atcagccgcg atcagcgcac cggtgtcata cgtgacgccg
     6241 ttcatcaggc gctcttgcgg gccggctgcc ggcgcagcat cgacctcgct cgggcgagtc
     6301 cctcggtgat ctcatccggc gtgagggcgc cgtgttggcg ttcccattcg gcgacgcctc
     6361 ggcggccggc nnnnnnnnnn accagccgac ggcgctgggc caggctgccg aggggatcca
     6421 cgcgctgacc gtcgtgcctt gcgcggtggc cgcgacgtca atcgcgtcgg cgaggtcggc
     6481 ggggagtgag atcgatcgct tctgtcgttg agccacggcg aaatggtagg acagaaagta
     6541 ggacagagca acgggagatc ctgcacgatt cctgcacagg attcggccgt acttgtcgga
     6601 cacgcccgga tcagacggac acgggcggac aacagcagcg gacgtgagca ggttggacgg
     6661 acgccggcgg accaaacgga cccgcagtcg aagtcttaaa cccccctctc cgcaaggagg
     6721 tgcgggttcg acccccgccc cgggcacttc cccaccactc gctctcgcgc cgcgtcgagc
     6781 gcacacctcc ggagcgcctc gcccgctacg tcgaactgtt gcagtcagag atgcccaaca
     6841 ccttggcgat cctcgtgccg ttcttggccg agtgagtcgg cttcgctatg cctcgtcgag
     6901 gaagtcccag gtcagcaccg cttcgcgacg ccgttcatcg gcacgccggt atgccaggac
     6961 atccccgagc cgcattcgcc gcttcgtgcc ggctcgggtc gacgggatct cgccgcggtc
     7021 gagcagggtc gtcagccacg gccgcgagac cccgagcagt tccgaggcga gttcggtacc
     7081 gatcggcagg ccggcaggtg cggagatgac ctcgcgcccg tacgacacgt cgtcgagcaa
     7141 cgttgcaacg agtgcgacga gctccggccc gagcgggtag ttccggccac cgatcgaaac
     7201 ggcgatgtcg gcaagtccgt ccgcccggat tgcatcaacg atctcggcag cgttgcgcgc
     7261 ctcggagcgc tcacgtcgat cggtggtcag ggccatgcaa ccagtcaacc atgtgtttcg
     7321 attaatcgca aggcgcctcg aactcaggat ccctgtcccg tcgcgttcgc gacccgccga
     7381 cttccggagg tgccagctgg caagccagtg actcccggct gaagcgcccc cggtttggtg
     7441 accgcgggga gcggccatcg ttacgaacga aacgcggcga ggaacgcttc agcttcctcg
     7501 tcggtgaggt cgtcgatcac catttcacct aacggcggca acggacgtgc gcgccggagc
     7561 aactcggcga ccggtacctc gaggctctcg gggtgaggta ctggcacggc gtcgttcgac
     7621 atctcgctcg ccacgctact aatcggcaca ccccaaggcc ggaagccatc ttcatcgagt
     7681 ccgacctcga ccgcatcttc ccaaccgtcg accgcctcac ccgatcggcg agcctctgaa
     7741 gatcggcgag tcatcacaag gtcagagcag ggatcgaagt cgcaggcctc gaaccttgtc
     7801 gaaatccttg aggttgcggg tggaaagttg gagatttcgt tcgagcgctg ttgcggcgat
     7861 caaggcatcg gggagccgaa gcccgaactc gcgcgccaca cgt
//
LOCUS       DAORNF010000038         7888 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_6785_length_7888_cov_1.922124, whole genome shotgun sequence.
ACCESSION   DAORNF010000038 DAORNF010000000
VERSION     DAORNF010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 7888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7888
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6785_length_7888_cov_1.922124"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>251)
                     /locus_tag="PLV68_02610"
     CDS             complement(<1..>251)
                     /locus_tag="PLV68_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00161.1"
                     /translation="KQFTLDHTSAGPDQSGSVQAVADADGVIVVAVERSLYRMRDDKP
                     EIVDAGVGTIGHLTAFGHGLVAVDGEDGLVVFDTTGDEL"
     assembly_gap    252..374
                     /estimated_length=123
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<375..1098)
                     /locus_tag="PLV68_02615"
     CDS             complement(<375..1098)
                     /locus_tag="PLV68_02615"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HRE00162.1"
                     /translation="MDQGGQRGAESELADGVLDDLGHSEAARTEEGYGYYALTDGDDP
                     DAGYGDELTDAKDEGNRRVPFAERNWRVPTVSVLLIAIVVIGVVIVLLPNDGRVDRSE
                     AAVGSFRDKPKERVSTSVAGTIVALETNDDSIYVLSVVNRSLRLVSLDRSTGDERWNV
                     SLEALGGARSSMIPRVVGDLWADADSLAVVSVVGAADPRYRAIDAESGAVTWSAQLRE
                     GSAPAFERELLVSTRSADGVTVT"
     gene            complement(1202..3835)
                     /locus_tag="PLV68_02620"
     CDS             complement(1202..3835)
                     /locus_tag="PLV68_02620"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRE00163.1"
                     /translation="MTDLAEPTTATGDAIDRVDLVVNGVPVSVRANHPHLLSALRDGL
                     DITSPKDGCSPSGQCGCCTVMIDGKAVVSCQQPLSKVAGKHITTLEGVDQAERERYAN
                     AFAACGGLQCGFCIPGIVMRAKSQIDKKGADLTRDDMARHLGANLCRCTGYVKILDAI
                     EAVAKGTDLTPPALANKVGGCGVKYEAAELTLGDRGYVDDIRVPGLLHAALHLTAHAR
                     AGIVSIDTTAAAAAPGVRAVYTAADIPGETMVGIIYKDWPVMIPIGGRTSYAGDVLAI
                     VVADTREHARAAVGLVDVTYEVLQPITDPLAAIEPGAEVAVWGTKDNVLSVSEYARGD
                     VDTALESSVHTVHEVFQTQRIEHAFLEPESTVAIPGPDGVLQVYSGGQGVWDDRNDIA
                     RMLGVGNEQVTVTLVSNGGAFGGKEDMSNQAHASLAAWLLNQPVKCTLSREESFRMHA
                     KRHPIRLEYWAGCDADGLLTAVRVRAVGDSGAYASVGMKVLERAAGHASGPYRVANID
                     VRAVAARTNNPVCGAFRGFGANQAQFGMEGVLDRLAEQVGISGWEIRKRNIIHPGEVW
                     GPGQQMDDGCAGAEACLDAAKPRYDEARAAGKAVGLALGLKNSGLGNGFREVSRAVVR
                     FRSTADRQGRDIEVRHGWTEMGQGVHTVALQVAAQELQVDPNRIEVIVDTTRELGFGQ
                     TTGSRGTLMAAGSVQQACASALAGGCELDVDYEGEHLVDWTQKIGDPAHPQPLIHAAF
                     GYAVQMAIVDRESGEIEEMVAIHDVGRAVNPLLCTGQIEGSLHMGLGYALSEQFPHDP
                     ETGFPTNATLRSLGILRAKDVPKLTVSLVEVPQPRAPFGIKGVGEIGLVPTAPAVAAA
                     LHDLDGQWRNTLPMRPKNASD"
     gene            complement(3915..4856)
                     /locus_tag="PLV68_02625"
     CDS             complement(3915..4856)
                     /locus_tag="PLV68_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoyl-D-amino-acid hydrolase"
                     /protein_id="HRE00164.1"
                     /translation="MPRILTVAAAQLGPVQADHSRAQVVERLIALLHEAHHHGVELVV
                     YPELALTTFFPRWFVDDITEADHWYEREMPNADTQPLFDEAKRLGIGFCLGYALLQTD
                     PDGTVHRHNVQTLVERDGSIVATYRKVHIPGHEHHEPDRPFQHAERYYFEPSTQGFGV
                     WRAFGGLMGMAICNDRRWPETYRVMGLQGVEMILCGYNTPIHYVPDPSQDILQGFHNA
                     LVMQAGAYQNGTWVVGVAKGGVEEGVDSLGQSMIVAPSGQIVAQALSTDDELVVARCD
                     LDWCQRYKGTLFDFDRYRRPELYGRITSQRGVVLPGD"
     gene            complement(4907..>6568)
                     /locus_tag="PLV68_02630"
     CDS             complement(4907..>6568)
                     /locus_tag="PLV68_02630"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HRE00165.1"
                     /translation="EPVVFQTASSAERLESLVAAVRNAAAMGRIERPHRPWLPPLGAD
                     TDTADAFGGSHTSSTDGPDSMGTSSPDTTSPGSRTIDGVVGVIDDPDNQCRRPLRWSP
                     ADGHLLLVGATGSGTTTALLEVATACAERDPGVELFVVDALGDVRLGLLDFLPQCAGV
                     VRVHERERLMRALAMVSGEIAIRKAMPECRTHDVVVCIDGLGALRNDLDAADERALID
                     QLHVIVTEGQTVGVSVVATAAGSTGVATALVTAMTRRWVFRLADSLDAAYFGLTAATA
                     LISGSGTGAEIGAGRLIDVSCGLEAQIVATAEQRLADLAHRPGRALAECRRRIEVLAP
                     AVPLAGLPQSSRVDGDLALTCARSFTTLDPVTLVVPAGEHVLVVGPARSGRTTALATL
                     VERWHQVSPDGVIVSVATRRDSLRVGQVTASVGEALGVVDAVLAGDRPLAHALGGGSV
                     LVVIDNAEAVDDSDGRLTAMAAARDPRCTILAAARPDALRAAYGHWTATVRRSRLGFV
                     MAAGQDIDGDLFGVVLPRRVPIAARPGLAWCVSSAGIELCQVASE"
     assembly_gap    6569..6585
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6586..6948)
                     /locus_tag="PLV68_02635"
     CDS             complement(<6586..6948)
                     /locus_tag="PLV68_02635"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HRE00166.1"
                     /translation="MREAGASDLGRFRVVRRERSKAGLHDLPALARLVVVIDEFATLA
                     TSQPAFLGALLGVAQRGRSLGVHLVLATQRPSGVVSDDISANTNLRIALRLQDRAESN
                     DVVGDAAAAHLPRSIPGRA"
     gene            complement(6840..>7344)
                     /locus_tag="PLV68_02640"
     CDS             complement(6840..>7344)
                     /locus_tag="PLV68_02640"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HRE00167.1"
                     /translation="AQIARRWASAPVDPSPSVPIGLASDGVVAIDLVADGPHTLIAGT
                     TGSGKSELLRSFVIGLACRFSPEYVNFVLVDFKGGSAFDACAALPHVVGLVTDLDGRL
                     ADRALRSLGQVAAATDRPASTPNCVAASTCCVRPGQATSAVSVSFAANGAKPVCTICP
                     PSPDSSW"
     assembly_gap    7345..7564
                     /estimated_length=220
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7565..>7888)
                     /locus_tag="PLV68_02645"
     CDS             complement(<7565..>7888)
                     /locus_tag="PLV68_02645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00168.1"
                     /translation="GPADWQLMIVTDHRRRWDGLTWLPHGEDVGRVPLIATTAEVAEL
                     IGRVEDDPRHLVVVTDRPDLLSVRTSPLRRLIAGERSCAVVVVCADEAALPSVATSAL
                     VVGSRG"
BASE COUNT         1159 a         2673 c         2555 g         1141 t
ORIGIN      
        1 gcaactcgtc accggtcgtg tcgaacacga ccagaccgtc ctcgccgtcg acggcgacca
       61 gcccgtgtcc gaacgcggtg aggtggccga tcgtgccgac cccggcatcg acgatctccg
      121 gtttgtcgtc acgcatccgg tagagcgagc gctccacggc gacgacgatc acaccgtcgg
      181 catcggcgac cgcctgcacg gagccggact gatcgggacc ggcgctggtg tggtcgaggg
      241 tgaactgctt gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnctgtca cggtgacgcc atctgccgac cgggtggaga ccaacagctc
      421 tcgttcgaag gccggtgccg agccctcccg gagttgtgcg ctccacgtca cagcaccact
      481 ctcggcatcg atcgcccgat accgcgggtc ggctgcaccc accaccgaca cgaccgcaag
      541 cgaatcggca tcggcccaca ggtcgccgac gacccgcggg atcatgctgg accgggcccc
      601 acccagtgct tccaacgaca cgttccagcg ctcgtcgcca gtgctccggt cgaggctgac
      661 gagccgcagc gaacggttga cgaccgacag cacatagatc gagtcgtcgt tcgtttcgag
      721 tgcaacgatc gtcccggcga ccgacgtgct gactcgctcc ttgggcttgt cacggaagct
      781 cccgacagca gcttccgaac ggtcgacccg tccgtcgttg ggcaagagca cgatcacgac
      841 accgatcacc acgatcgcga tcagcaacac gctcacggtg ggcacgcgcc agttgcgctc
      901 ggcgaagggc acacgccggt tgccctcgtc cttggcatcg gtgagctcgt cgccgtagcc
      961 cgcatcgggg tcgtccccgt ccgtgagggc gtagtagccg tagccctctt cggtgcgagc
     1021 ggcctcgctg tgcccgagat cgtcgagcac gccgtctgcc aactcgctct ctgcgccgcg
     1081 ctggcctccc tggtccatcg cccgccagtc tcgcagagag acacacggta ctggcagcga
     1141 tcggagctgg cagcgacggg gggttggctg cgatcagccg gggcgaatca gtcggtcccg
     1201 atcaatcgga cgcgttcttg gggcgcatcg gcagggtgtt gcgccactga ccgtcgaggt
     1261 cgtgcagcgc ggcggctacg gcgggggcgg tgggcaccag gccgatctcg ccgacgccct
     1321 tgatgccgaa cggtgcccgc ggctggggca cttcgaccag cgacacggtc agcttcggca
     1381 cgtccttggc gcgcaggatg cccagcgagc gcagtgtggc gttggtgggg aagccggtct
     1441 ccggatcgtg ggggaactgc tcggacagtg catagcccag gcccatgtgc aggctgccct
     1501 cgatctggcc cgtgcacagc agcgggttga ccgcccggcc gacgtcgtgg atcgcgacca
     1561 tctcctcgat ctcgcccgac tcccggtcga cgatcgccat ctgcaccgca tagccgaacg
     1621 cggcgtggat cagcggctgc gggtgggcgg ggtcgccgat cttctgcgtc cagtcgacta
     1681 ggtgctcacc ttcgtagtcg acgtcgagtt cgcacccgcc ggccagggcc gaggcgcacg
     1741 cctgctgcac cgacccagcg gccatcaacg tgccacgcga tcccgtggtc tggccgaaac
     1801 cgagctcgcg ggtggtgtcg acgatgacct cgatccggtt cgggtcgacc tgcaactctt
     1861 gcgctgccac ctgcagggcg acggtgtgca cgccctggcc catctcggtc cagccgtggc
     1921 gcacctcgat gtcgcgaccc tgccgatcgg cggtgctgcg gaagcggacg acggcgcggc
     1981 tgacctcgcg gaaaccgttg ccgaggccgc tgttcttcag gccgagggcg aggccgacgg
     2041 ccttgcccgc cgcgcgagcc tcgtcgtagc ggggcttggc cgcgtcgaga cacgcttcgg
     2101 caccggcaca cccgtcgtcc atctgctggc ccggacccca cacctcgccc gggtggatga
     2161 tgttgcgctt gcggatctcc cacccgctga taccgacctg ctcggccaag cggtcgagca
     2221 cgccttccat gccgaactga gcctggttgg caccaaagcc acggaaggcg ccgcacacgg
     2281 gattgttggt tcgcgccgcg accgcgcgga cgtcgatgtt ggccacgcgg tacgggccgc
     2341 tggcgtgccc ggcggcgcgt tcgagcacct tcatgccgac gctggcatag gcgccagagt
     2401 cgccgacggc gcggacgcgc accgcggtca gcaatccgtc ggcatcgcat cccgcccagt
     2461 actccaaccg gatcggatga cgcttggcgt gcatccggaa gctctcctca cggctgaggg
     2521 tgcacttcac cggctggttg agcagccacg cagcgagcga ggcatgggcc tggttgctca
     2581 tgtcctcctt accgccgaag gcgccaccgt tggacaccag cgtgaccgtg acctgttcgt
     2641 tgccgacgcc gagcatgcgg gcgatgtcgt tgcggtcgtc ccacacaccc tggccaccgc
     2701 tgtacacctg cagcacccca tcggggccgg ggatcgcgac ggtgctctcc ggttcgagga
     2761 acgcgtgctc gatccgctgg gtctggaaca cctcgtgcac ggtgtgcaca ctggactcga
     2821 gcgcggtgtc gacatcgccg cgggcgtact cgctgacgct gagcacgttg tccttggtgc
     2881 cccacaccgc cacctcggca ccgggttcga tcgcggcgag cggatcggtg atcggctgca
     2941 ggacctcgta ggtgacatcg accaggccga ctgcggcgcg ggcgtgttcg cgcgtgtcgg
     3001 ccacaacgat cgccagcaca tctccggcgt agctggtgcg gccgccgatc gggatcatca
     3061 ccggccagtc cttgtagatg atgccgacca tcgtctcgcc ggggatgtct gccgcggtgt
     3121 agaccgcgcg aactcccgga gcggccgctg cggcggtcgt gtcgatcgat acgatgccgg
     3181 cccgggcgtg agcggtgagg tgcagcgcgg cgtgcagcag acccggcacg cggatgtcgt
     3241 cgacatatcc gcgatcgccc agcgtcaact cggccgcttc gtatttgacg ccgcacccgc
     3301 cgaccttgtt cgccaacgcg ggtggtgtca ggtcggtgcc cttggccacc gcttcgatcg
     3361 catcgaggat cttgacgtac ccggtgcagc ggcacaggtt ggcacccagg tgacgggcca
     3421 tgtcgtcgcg ggtgaggtcg gcgcccttct tgtcgatctg cgacttggcc cgcatcacga
     3481 tgccggggat gcagaagccg cactgcaggc cgccgcatgc ggcaaaggca ttggcgtagc
     3541 gctcgcgctc ggcctggtcg acgccttcca gcgtggtgat gtgcttgccc gcgactttgc
     3601 tgagcggctg ttggcagctg acgaccgctt tgccgtcgat catcaccgtg cagcagccgc
     3661 actgcccgct gggcgagcag ccgtccttgg gggaggtgat gtcgaggccg tcacgcagcg
     3721 cagacagcag gtgcggatgg ttcgcgcgca cgctgaccgg caccccgttg acgacgagat
     3781 cgacccggtc gatcgcgtcg ccggtggctg ttgtgggttc ggccagatcg gtcacgcttg
     3841 acattttgtc aaagccagcg gcgaagggga acacacgggc gcagccgaac ccgtcggccc
     3901 gcgcgccgac cgctctagtc gccaggcagc acgacgccgc gctggctggt gatccggccg
     3961 tacaactcgg gacggcggta gcggtcgaag tcgaacaagg tgcccttgta gcgctggcac
     4021 cagtcgagat cgcagcgggc gacgacgagc tcgtcgtctg tgctcagcgc ctgagcgacg
     4081 atctggccgc tcggcgcgac gatcatcgat tgccccagcg agtcgacgcc ttcctcgact
     4141 ccgcccttgg ccacgcccac gacccaggtg ccgttctgat acgcaccggc ctgcatcacc
     4201 aacgcgttgt ggaagccctg gaggatgtcc tggctggggt cgggcacgta gtggatcggc
     4261 gtgttgtagc cgcacaggat catctccacc ccctgcagcc ccatcacccg ataggtctcc
     4321 ggccagcggc ggtcgttgca gatcgccatc cccatcaggc cgccgaaggc ccgccacacg
     4381 ccgaagccct gggtgctcgg ctcgaagtag taccgctcgg cgtgttggaa cggccggtcg
     4441 ggttcgtggt gctcgtgacc ggggatgtgc accttgcggt aggtcgccac gatcgagccg
     4501 tcacgctcga ccagggtctg cacgttgtgg cggtgcacgg tgccgtcggg gtcggtctgc
     4561 agcagggcgt agccgaggca gaacccgatg ccaagccgct tggcttcgtc gaacagtggc
     4621 tgcgtgtcgg cgttgggcat ctcccgctcg taccagtggt cggcttcggt gatgtcgtcg
     4681 acgaaccagc gcgggaagaa cgtcgtcagc gccaactcgg gatacaccac cagctcgaca
     4741 ccgtggtgat gtgcctcgtg caacagcgcg atcagacgct cgacgacctg ggcgcgggag
     4801 tggtcggctt ggacggggcc gagctgggcg gcggcgacgg tcaggatgcg cggcatacgc
     4861 cgcccacact gacaccatca actgggctgt cgtcaagtgg gaggcgtcac tcgctggcga
     4921 cctggcacag ctcgatgcct gcactcgaca cgcaccatgc caggccgggg cgcgccgcga
     4981 tcgggacgcg ccgcggcagc accacgccga acaggtcacc gtcgatgtcc tgaccggcgg
     5041 ccatcacgaa gccgagccgc gaccggcgca ccgtcgcggt ccagtgtccg tacgccgcac
     5101 gcagagcgtc gggccgggcc gcggcaagga tcgtgcaccg tgggtcgcga gcagcggcca
     5161 tcgcggtcaa gcggccgtcg ctgtcgtcca ccgcctcggc attgtcgatc accaccagca
     5221 cagagccacc gcccagcgcg tgggccagcg ggcggtcgcc ggccagcacc gcgtcaacga
     5281 caccgagcgc ctcaccaacc gacgcggtga cctggcccac gcgcagcgag tcacgccgcg
     5341 tcgccaccga cacgatcacc ccgtcggggc tcacctggtg ccaacgctcg accagcgttg
     5401 ccaacgctgt ggttcgcccg ctgcgcgccg gtcccaccac cagcacatgc tcaccagcgg
     5461 gcaccaccaa cgtcacggga tcgagcgtgg tgaacgaacg cgcacacgtc agcgcgagat
     5521 cgccatccac ccgcgacgac tggggtagcc cggccaaggg caccgcggga gcgagcacct
     5581 cgatgcgacg acggcactcg gccagcgccc ggcccggtcg gtgggcgaga tcggccaggc
     5641 gctgctcggc cgtcgcgacg atctgagcct ccaagccgca cgacacgtcg atcagacggc
     5701 cggcgccaat ctcagctccg gtcccgctcc cgctgatgag cgccgtggcc gcggtcagcc
     5761 cgaagtacgc ggcgtcgagc gagtccgcca ggcggaacac ccagcgccgc gtcatcgccg
     5821 tgacgagcgc cgtcgccaca cccgtcgagc cagcggcggt cgcgacgacg ctgacgccga
     5881 cggtctggcc ttcggtgacg atcacgtgca gttgatcgat cagcgcgcgc tcgtcggccg
     5941 cgtcgaggtc gttgcgcagc gcgcccaggc cgtcgatgca gaccaccacg tcgtgagtcc
     6001 ggcactcggg catcgccttg cggatcgcga tctcgccact caccatcgcc agcgcgcgca
     6061 tcagccgctc gcgttcgtgc acccgcacga ccccggcgca ttgcggtagg aagtcgagca
     6121 ggccgagccg gacgtcgccg agcgcatcga ccacgaacaa ctcgacgccg ggatcacgct
     6181 cggcgcacgc agtggccacc tccaacaacg cggtggtcgt gccgctccct gtcgccccca
     6241 ccaacagcag gtggccgtcg gccgggctcc accgcagcgg tcggcggcac tggttgtccg
     6301 ggtcgtcgat cacaccgacc acaccgtcga tggtgcggga gcctgggctg gtggtgtctg
     6361 ggctgctggt gcccatgctg tcgggaccgt cggtgctgct ggtgtgtgag ccgccgaacg
     6421 catcggccgt gtcggtatcc gctcccagcg ggggaagcca cgggcggtgc ggacgctcga
     6481 tgcgccccat cgctgcggcg ttacgcacgg ccgcaaccaa cgactccaac cgctccgccg
     6541 acgaggcggt ctggaacacg accggctcnn nnnnnnnnnn nnnnncgccc gtccggggat
     6601 cgagcgcggc aggtgcgcgg ccgcagcgtc gccgaccacg tcgttggact cggcccgatc
     6661 ctgcagccgc agggcgatgc gcaggttggt gttggcgctg atgtcgtcgc tcaccacacc
     6721 gctcgggcgc tgagtcgcca acaccaggtg cacgcccaag ctgcgtcccc gctgcgcgac
     6781 gcccagcagt gccccgagaa acgccggttg gctcgttgcc aacgtcgcga actcgtcgat
     6841 caccacgacg agtcgggcga gggcgggcag atcgtgcaga ccggctttgc tccgttcgcg
     6901 gcgaacgaca cggaaacggc cgaggtcgct tgccccggcc tcacgcagca ggtgctcgcg
     6961 gcgacgcagt tcggcgtcga ggccggtcga tccgttgccg cagccacctg cccgaggctg
     7021 cgcagggcac ggtcggcaag acggccgtcg agatcggtga ccagcccgac cacgtgcggc
     7081 aacgccgcgc aggcatcgaa ggccgaccca cccttgaaat cgaccagcac gaagttgacg
     7141 tactcgggac tgaaccggca ggcgaggccg atcacgaagc tgcgcaacag ttcgctcttg
     7201 cccgagccgg tggtgccggc gatcagcgtg tgcggcccgt cggccaccag gtcgatcgcc
     7261 accaccccgt cactggccag gccgatcggc accgatgggc tgggatcgac cggtgcgctc
     7321 gcccagcggc gggcgatctg ggcgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7561 nnnnccccgc gagccgacga ccaacgcgct ggtcgccacg ctgggcagcg ccgcttcgtc
     7621 cgcgcacacc acgaccaccg cgcacgagcg ttcgccggcg atcaggcgac gcagcggcga
     7681 ggtgcgcacc gacagcaggt ccggccggtc ggtgaccacg acgaggtggc gggggtcgtc
     7741 ttcgaccctg ccgatcagtt cggcgacctc ggccgtcgtc gcgatcagcg gcacccggcc
     7801 gacatcctcg ccgtgcggca gccacgtcag accgtcccac cgccggcggt gatcggtgac
     7861 gatcatcagc tgccagtcgg ccgggccg
//
LOCUS       DAORNF010000039         7851 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_6836_length_7851_cov_1.858902, whole genome shotgun sequence.
ACCESSION   DAORNF010000039 DAORNF010000000
VERSION     DAORNF010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 7851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7851
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6836_length_7851_cov_1.858902"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="PLV68_02650"
     CDS             <1..235
                     /locus_tag="PLV68_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HRE00169.1"
                     /translation="IIGFEGLNHEQGFELAVALHQWQTRDEFVYRHQWQPGMLTMWDN
                     RSVLHRATGGYEGYDRELHRTTIGHNPTFAHHP"
     assembly_gap    281..429
                     /estimated_length=149
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <430..837
                     /locus_tag="PLV68_02655"
     CDS             <430..837
                     /locus_tag="PLV68_02655"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRE00170.1"
                     /translation="DIVQDTFIAVGQRLDVVPADKERAWVLGVARNIARNARRSRHRF
                     HRFTDALAATLATQASHLYQDRPTIEMTDTVHSAFGQLSTSDQEIVLLAAWHDLAPAD
                     ISVVLDISPERASDRLYNARKRFRKHYDRAGGT"
     gene            936..1565
                     /locus_tag="PLV68_02660"
     CDS             936..1565
                     /locus_tag="PLV68_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00171.1"
                     /translation="MLARLDDPPPQQPQRRWRWRRRSVVAGLVVVGIAGTASVAIAAW
                     YSTSTPRDPLDVACWSDQQRSSQWEVRIGLGDSPLTACGTPWDDGTFGRSGPPPLVAC
                     TSAEGIIAVVPAESCAKVGLDGLTPGYEDSTSAQTADLLQQLIGDRLFEECHTEVETR
                     SLIDGVLAEHGLDDWTVESPVPFTADRPCGQVAIDPTTRTITIVTIRSH"
     gene            1593..2168
                     /locus_tag="PLV68_02665"
     CDS             1593..2168
                     /locus_tag="PLV68_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00172.1"
                     /translation="MLLHILAATAFTVCSTPGFPWETSRPSASDAAQQTPKSVGDPAH
                     ARFNGRAIDLARSWEGAHACIQFDTETNCYETEAMWEAAHPDHASPAPATNHQAAPLA
                     TCATALKLYDGTSYTGTSLFITTRGAILNLSTFGFDNRASSYKVGTCSADFFAGANGL
                     GGVYPGNTNAGASAASMLSGWDNTVSSVYLY"
     gene            2269..2598
                     /locus_tag="PLV68_02670"
     CDS             2269..2598
                     /locus_tag="PLV68_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00173.1"
                     /translation="MKKKVLTGAIVLAATLFGSATSADAYVAVNPNTGGSCTFTGGRS
                     GTNVWASTSTCDAVHTRITMHVDGSNTTYYGISCCPASSVAGGTGTFAGAAIRAKLGT
                     WSSWYAV"
     assembly_gap    2663..2681
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2682..3061
                     /locus_tag="PLV68_02675"
     CDS             <2682..3061
                     /locus_tag="PLV68_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00174.1"
                     /translation="SNSPRSPITRQALVEAQQQVVDCLAARGWDASYVDDYTLSVKGR
                     SGDDVDAQSVDVDACSSDAQAVIDEFARQQMPDQNTIDAALLTCLRSHGIPLDDLSGT
                     VIEELSETHPDEMAICYAAALRP"
     gene            3058..4077
                     /locus_tag="PLV68_02680"
     CDS             3058..4077
                     /locus_tag="PLV68_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HRE00175.1"
                     /translation="MMRRAAPIVLVLLVSAACAIAAVLAWRQAVRTTDAALPGFASTE
                     SAAGHQRYDGQRDARLQLSLDDGGVLTSPATGLITRSECSPNSTLTSGQLFAEVDGAW
                     MVALSTSQPLWRDLARGSKGRDVTNLQEELVRLGYTLVPTGVFDRQTDDALIALAYGL
                     GYAHESGFRATQALWLPSAGVELATCELAVGSPITIGTPVATGPPGVSAAALSISRPP
                     NDNITGAWEFRSGEVIVPLDDDRYPLIDADQLDAIVAASSAQPDGSRVLDGAVRLVTP
                     IDVLAVPPAAVVALDTQHGCVVDVATGSPVEVDLIDSELGRTLITAAPVGLMSVALDP
                     IACRA"
     gene            4074..>4385
                     /locus_tag="PLV68_02685"
     CDS             4074..>4385
                     /locus_tag="PLV68_02685"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRE00176.1"
                     /translation="MNTDALVEVDNLTFAYRRQPPTITELSFRVDAGSRTALVGPSGS
                     GKSTMLALIAGLLRPQTGSLHRAAGERELCWVGQTPVGVSRRSAIDHVRLIVACHSWP
                     DS"
     assembly_gap    4386..4461
                     /estimated_length=76
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4462..4695
                     /locus_tag="PLV68_02690"
     CDS             <4462..4695
                     /locus_tag="PLV68_02690"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRE00177.1"
                     /translation="RFGKLSGGEAQRLMLAMAVAAQPRLLLIDEPTAQLDRATALDVT
                     SVIAKLSEFGTTVIVATHDDAVRAVCNAVVSLG"
     gene            4706..5092
                     /locus_tag="PLV68_02695"
     CDS             4706..5092
                     /locus_tag="PLV68_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00178.1"
                     /translation="MTLRAIGREAVLNLWTRAAAPLAQLAVFILAVVGDTGLLASRLT
                     DIADRHANIVDHGGATAIATSAASVDANGRTASLASLVRRRCEPREWSDSRPFPTCHF
                     NSYRQARRSGRSYPSQRATRTSRGSR"
     gene            5089..5772
                     /locus_tag="PLV68_02700"
     CDS             5089..5772
                     /locus_tag="PLV68_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00179.1"
                     /translation="MSADVAAQLGLAVGDQTVASLDGEPTVLPIAVTFHVPWPNSRLD
                     DAVITLVSPTRQFDQCVVAAAGWASDTRSLAAVAASATTDNPDFLGRANPSVGVAPDF
                     GSLLRGAGAGLLAAAVGLASAIATLAQRSRRHLFTSVASHMGLRTTDSLAIALTEQVL
                     LAPARFAIAAVTTWAFTETNERLDVRNVLHDWTITASLLAVVGAFIATVIVAPLGSKR
                     ATLKRFRTR"
     gene            complement(5919..6173)
                     /locus_tag="PLV68_02705"
     CDS             complement(5919..6173)
                     /locus_tag="PLV68_02705"
                     /inference="COORDINATES: protein motif:HMM:NF016430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HRE00180.1"
                     /translation="MGGSHAWTDVSSVPRYFVDVYNDRYRVLVEIARLTTGSNALAEE
                     IVQDAFVDLYRRFDTLHSPEAYVHRATNPQASTSPGTTTA"
     gene            complement(6185..>7398)
                     /locus_tag="PLV68_02710"
     CDS             complement(6185..>7398)
                     /locus_tag="PLV68_02710"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRE00181.1"
                     /translation="SRWRRFWRLDVPHGAIGLVWNGMMSMGGGWFFLVASEAISLANH
                     TYALPGVGAYAGAVIDDGDLGKIGLAVVTMVVMVVAVNVVFWQPLVAWCEKFKNEQSE
                     AAEVPRSFVLDVLRSSHWPRALGRARRRVAEPVNRLCDAIFGTATKLPEPAPLMGHRR
                     SDVTFWVIATAGLGIGAWRLFAYIVEADGWGVFWTPLWQGAITLVRVAVLMIVATVVW
                     VPIGVLIGSKPRVRRIAQPIVQVLASFPANFLFPFVTWVLLRTGIGLDIGGIALMALG
                     AQWYILFNVIAGAQSIPTDLGEAMDDLGVHGRQRWQRMVLPAIFPHYVTGAIAASGGA
                     WNASIVAEVVTYGGTTLTATGLGAFIAHATEAGDFHRILAGIAVMAVYVVSLNRLVWR
                     PLQRLAESRYA"
     assembly_gap    7399..7440
                     /estimated_length=42
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7441..>7851)
                     /locus_tag="PLV68_02715"
     CDS             complement(<7441..>7851)
                     /locus_tag="PLV68_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00182.1"
                     /translation="NSYIRASARCSVGRGTTLSFTPTDVTAVDTSPSRLPYDAARSLL
                     RMFIALGASYLFAFVYASIAAHSRRAERLMIPALDILQSVPVLGFLAVTVTGFIALFP
                     GSYLGLELAAIFAIFTSQAWNLTFSFYDSLVTQPR"
BASE COUNT         1325 a         2591 c         2402 g         1247 t
ORIGIN      
        1 catcatcggc ttcgagggtc tgaaccacga gcaaggcttc gagctcgcgg tcgcgctcca
       61 ccagtggcag acccgcgacg agttcgtcta ccgccaccag tggcagccgg gcatgttgac
      121 gatgtgggac aaccgctcgg tgctgcaccg cgccaccggc ggctacgagg gctacgaccg
      181 cgagctccac cgcaccacca tcggccacaa ccccaccttc gcccaccacc cgtagacccg
      241 acgatttttc gggtttgcgg cgagattggg gcgtgagcgg nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnng acatcgtgca ggacacgttc attgcggttg gacaacggct tgacgtggtg
      481 ccagccgaca aggagcgggc atgggtgctc ggtgtcgccc gcaacatcgc tcgcaacgcc
      541 cgtcggtcgc gtcaccggtt ccatcggttc accgatgctc tcgccgccac gcttgcgaca
      601 caggcgagtc acctctacca agatcggccg accatcgaga tgacggacac cgtgcacagc
      661 gcgtttggtc aactcagtac atcagaccag gagatcgtgc tcttggcggc gtggcacgac
      721 ctggcacccg ccgacatctc ggtcgtactc gacatctcgc ctgaacgggc cagtgatcgg
      781 ctgtacaacg cgcgcaagcg tttccgcaag cactacgacc gggccggtgg gacatgagtt
      841 cacctcgcga tccgttcgac cttctccgct cgatcgcccc cggtcgcgcg cctgaccacc
      901 agccagcagc cgttggcgat ccgatcctgc agcgcgtgct cgcccgcctt gacgatccgc
      961 cgccgcaaca accacagcga cggtggcgct ggcggcgccg cagtgtcgtc gcgggtttgg
     1021 tcgtggtggg gattgccgga accgcctccg tcgcgatcgc tgcgtggtac tcgacctcga
     1081 cgccgcgtga cccgttggac gttgcgtgtt ggagcgatca gcagcgatcg agccaatggg
     1141 aggttcgtat cgggctcggt gactcaccac tcacagcctg cgggaccccg tgggacgacg
     1201 gcacgttcgg ccgttccgga ccaccacctc tggtcgcgtg cacgtcggcc gaagggatca
     1261 tcgccgttgt ccccgctgag tcctgcgcca aagtcggcct cgatggcctc acccccggct
     1321 acgaggacag tacctccgcg cagacggcgg accttctgca gcaactcatc ggtgatcgtc
     1381 tcttcgagga atgccacacc gaggtcgaga cgcgttcgtt gatcgacggg gtgctcgcag
     1441 aacacggact cgatgactgg actgtggagt cacccgtgcc cttcaccgct gaccggccgt
     1501 gcggccaggt cgccatcgac ccgaccacac gcaccatcac catcgtcacc atccgttccc
     1561 actagctcca gacacaccag gaaacccact caatgctgct tcacatcctc gccgccaccg
     1621 ccttcaccgt ctgctcaacg ccgggtttcc catgggaaac ctcacgtcca tccgcgtcgg
     1681 acgcggccca gcaaacgcca aaatcagtgg gcgaccccgc gcacgctcga ttcaacggga
     1741 gagccatcga cctcgcgcgt agttgggaag gagcacacgc ctgcatccag ttcgacacgg
     1801 aaaccaactg ctatgagacg gaggcgatgt gggaggcggc gcacccggat cacgccagcc
     1861 cggctcccgc gaccaaccac caggccgcgc cactcgcgac ctgcgcaacc gcgctcaagc
     1921 tctacgacgg aacgtcctac acgggcacat cgctgttcat caccactcga ggcgcgatcc
     1981 tcaacctgtc gaccttcggg ttcgacaacc gagcctccag ctacaaggtc gggacctgtt
     2041 ccgctgactt cttcgcagga gccaacggac tcggcggcgt ctaccccggc aacaccaacg
     2101 cgggcgcatc agccgcatcc atgctgagcg gctgggacaa cactgtctcc agcgtctacc
     2161 tgtactgact tcgctgagtt ctttgcggtc gacggcgaga ttcaccctcg gccggacact
     2221 agccattaca agtccggcgg agtcatccgc cacaacctgg aggcagggat gaagaagaaa
     2281 gttcttaccg gggcaatcgt gctagcagcg acactgttcg gatcggcaac ttcggccgat
     2341 gcttacgtcg cggtgaatcc gaacactggc ggctcgtgca cgttcacggg cggccggagc
     2401 ggcaccaacg tgtgggcgtc cacctcgaca tgcgacgccg tccacactcg gatcaccatg
     2461 cacgtcgatg gcagcaatac cacgtactac ggaatctcgt gctgtcccgc gagttcagtg
     2521 gcaggcggaa caggcacctt cgccggcgct gccatccggg ccaagctcgg cacatggtcc
     2581 tcctggtatg cggtgtgacg tcacttgggc ggtgggggtt ggcgggggtg ttcgtggtcg
     2641 gggtgctcgg gtgctcatcg gcnnnnnnnn nnnnnnnnnn ntgtccaact cgccacgttc
     2701 gccgatcacc aggcaggcgc tggtcgaggc gcagcagcag gttgtggatt gtctcgctgc
     2761 ccggggttgg gacgcctcct atgttgacga ctacacgctc agcgtgaaag gccgatccgg
     2821 cgatgatgtt gatgctcaat cggtcgatgt cgacgcgtgc agcagcgacg cgcaagcggt
     2881 gatcgatgag ttcgctcgcc aacagatgcc cgaccagaac acgatcgacg ccgcgctgct
     2941 gacctgccta cggtcgcacg gcattcctct tgacgacctg tccggcacgg tgatcgagga
     3001 gctttccgag acccatcccg acgagatggc gatctgctac gcggcagccc tacgcccgtg
     3061 atgcgccgcg ccgcaccgat cgtgctggtg ctgctcgtga gcgctgcctg tgccattgca
     3121 gccgtgctgg catggcgaca ggccgtacgt acgaccgatg ccgcactgcc cggcttcgct
     3181 tcgacggaga gcgccgccgg ccatcagcgc tacgacggtc aacgcgatgc ccgactgcaa
     3241 ctcagcctgg atgacggcgg cgtcttgacc agcccagcga ctggcctgat cacccgctcc
     3301 gagtgctcgc cgaactcaac gttgacgtca ggccaactgt tcgccgaagt cgacggcgcg
     3361 tggatggtgg ccttgtcgac cagccagcca ctttggcgcg acctcgctcg cggctccaaa
     3421 ggccgcgacg tcaccaatct gcaggaagag ttggtacggc tggggtacac actggttcca
     3481 acgggggtct tcgaccgcca gaccgacgac gctttgatag cgctggcata cgggcttggc
     3541 tatgcgcacg agagcgggtt ccgcgcaacc caagccttgt ggctgccgag cgcaggcgtc
     3601 gagctcgcga cctgcgaact cgccgtcggt tcgccgatca ccatcggcac ccccgtcgcg
     3661 accggaccgc caggtgtctc ggcagccgca ctgtcgatct cgcggccacc gaacgacaac
     3721 atcactggtg cctgggagtt ccgctcgggc gaagtcatcg tcccgctgga tgacgatcga
     3781 tatccgttga tcgacgctga ccaactcgac gcgatcgtcg ccgcgtcgag cgcgcagccc
     3841 gatggatcgc gggttctcga cggagcggta cgactcgtca cccccatcga cgtgctcgcg
     3901 gtgccaccag cggctgtcgt cgcactcgac acccagcacg gctgcgttgt cgacgtcgcc
     3961 acagggtcac cggtcgaagt tgatctgatc gactcagaac tcggccggac gctcatcact
     4021 gcggcgccgg tcggcctcat gtcggtcgct ctcgacccga tcgcatgtcg tgcatgaaca
     4081 ctgacgctct tgtagaggtc gacaacctga ccttcgccta tcgccgtcag cctccgacga
     4141 tcaccgaact cagcttccga gtcgacgctg ggtcgcggac tgcgctcgtc ggcccgtcgg
     4201 gttcgggcaa gagcaccatg cttgcgttga tcgcaggtct gctgcgaccg cagaccggat
     4261 cgctgcaccg cgctgctggc gagcgcgagt tgtgctgggt tggtcaaacg cctgtcggcg
     4321 tcagccgccg gtcggcgatc gaccacgtac ggctcatcgt cgcctgtcac tcgtggcccg
     4381 actccnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4441 nnnnnnnnnn nnnnnnnnnn ncggttcggc aagttgtccg gtggcgaggc acagcgcctc
     4501 atgctggcta tggccgttgc ggcgcagcca cgcctgctcc tgatcgacga gccgacagcg
     4561 caactcgacc gcgccaccgc gctcgacgtc accagcgtga tcgccaagct ctccgagttc
     4621 ggcacaaccg tgatcgtggc gacgcacgac gatgctgtac gcgctgtctg caacgcggtg
     4681 gtgtcgcttg gctagcggcc atgcgttgac gctgcgcgcc atcggtcgcg aagcggtgct
     4741 caatctgtgg acccgcgcgg ctgcgcccct ggcacagctc gctgtgttca tcctcgcagt
     4801 tgtcggtgac accggtcttc tggcgtctcg gctgaccgac attgctgacc ggcacgccaa
     4861 catcgtggac cacggcggcg cgaccgccat cgcgacgtcc gccgcgagcg tcgacgcgaa
     4921 cggcagaacg gcaagcctgg cgtcgctggt gcggcggcgc tgcgaaccgc gggagtggtc
     4981 cgattcgcga cccttcccaa cttgccattt caactcgtac aggcaagccc gtcgatctgg
     5041 tcggtcctat ccgtctcagc gggcgacccg aacgtcgcgg ggatcgcggt gagtgctgat
     5101 gtcgccgcac agcttgggct cgctgtcggc gaccaaaccg tcgcctccct cgacggcgaa
     5161 ccgacggtgc tgcccatcgc ggtcacattc cacgtgccat ggccgaacag ccgactcgat
     5221 gatgcagtca tcacgctcgt gtcgcccacc cgccagttcg atcagtgtgt cgtggccgct
     5281 gcagggtggg ccagcgacac gagatcgctg gctgccgtgg ccgcttcggc caccacagac
     5341 aacccagact tcctgggccg cgccaaccca tcggttggtg tagcgcccga cttcggcagc
     5401 ctgctgcgag gtgccggggc gggactgctc gcagcggctg tcggtctcgc cagcgcgatc
     5461 gcgacacttg cgcaacggtc gaggcgtcac ctcttcacct cagtcgcatc tcacatgggt
     5521 ctgcggacca ccgactcgct cgcgattgcc ctcaccgagc aggtactgct cgcaccggca
     5581 cggtttgcga tcgccgcggt cacaacatgg gcgtttaccg agacgaacga gcggttggac
     5641 gtgcgcaacg tgctgcatga ctggacgatc actgcttcac tgctcgcggt cgtcggagcc
     5701 ttcatcgcca ccgtgatcgt cgctccgctc ggatcgaagc gagcaaccct caagcggttc
     5761 cgcaccagat agttggacag tccctgcggg tggggggtct gggtcagcac cttggggcga
     5821 tgttgcgagg gctgggtcca gtgctgacgc tcaggcacga gtgaggtggg tggccacggc
     5881 ggtgaccgtc gcggcgtcgg ggccggtgat gcggaaggtc aggccgtcgt ggttccaggt
     5941 gatgtagacg cctgcgggtt ggtcgcccga tggacgtacg cctcggggct gtgcagcgtg
     6001 tcgaagcgcc gatagaggtc gacgaaggcg tcctgcacga tctcctccgc gagcgcgttg
     6061 gagcccgtgg tcagacgagc gatctcgacc aacacccggt agcggtcgtt gtagacgtcg
     6121 acgaagtaac gcggaacgct ggacacatcc gtccaggcgt gtgacccacc cattcggttc
     6181 cacgtcaggc gtagcgagat tcggcaagcc gctggagcgg gcgccacacc aggcggttga
     6241 gcgacaccac gtacacggcc atcaccgcga tgccggcgag gatgcgatgg aagtcacctg
     6301 cctcggtggc atgcgcgatg aacgcgccga gcccggtggc ggtcagcgtc gtaccgccgt
     6361 aggtgacgac ctccgccacg atcgacgcgt tccacgcgcc accagatgcc gcgattgcac
     6421 cggtgacgta gtggggaaag atcgcgggca acaccatccg ctgccagcgc tgacggccgt
     6481 gcacgccgag atcgtccatc gcctcgccga ggtcggtcgg gatcgactgc gccccggcga
     6541 tgacgttgaa caggatgtac cactgcgcac ccaacgccat cagcgcgatg ccgccgatgt
     6601 cgaggccgat gcccgttcgc agcagcaccc acgtcacgaa cgggaacagg aagttggccg
     6661 ggaagctggc cagcacctgc acgatcggct gggcgatgcg gcgcacccgc ggcttggaac
     6721 cgatcaacac gccgatcggc acccatacca ccgtggcgac gatcatcagt acggcgacgc
     6781 gcaccagcgt gatcgcgccc tgccacagcg gtgtccagaa cacgccccac ccgtcggcct
     6841 cgacgatgta ggcgaacagc cgccacgcac cgatgccgag cccggcggtg gcgatgaccc
     6901 agaacgtcac atcggaacgg cggtggccca tgagcggcgc tggttcgggg agcttggtcg
     6961 cggtgccgaa gatcgcgtcg cacaaccggt tgaccggctc ggctacgcgc cgcctcgccc
     7021 ggcccagcgc acgcggccag tggctggacc gcaacacgtc gagcacgaac gagcgtggta
     7081 cctcggccgc ttcggactgc tcgttcttga acttctcgca ccacgccacc agcggctgcc
     7141 agaacacgac gttgacggcg acgaccatca cgaccatcgt caccaccgcg agaccgatct
     7201 tgccgaggtc gccgtcgtcg atcaccgcac cggcgtaggc gcccacgccg gggagcgcat
     7261 aggtgtgatt ggccaggctg atcgcctcgg acgcgaccag gaagaaccag ccgcccccca
     7321 tgctcatcat cccgttccac accagaccga tcgcgccgtg gggtacgtcg agacgccaga
     7381 agcgccgcca ccgcgacann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7441 cgcggttggg tgacgagcga gtcgtagaag ctgaacgtca ggttccacgc ctgcgaggtg
     7501 aagatcgcga agatcgccgc aagctccaga cccagatacg aaccgggaaa cagggcgatg
     7561 aacccggtga cggtgacggc caggaaaccg agaaccggta ccgactggag gatgtcgagc
     7621 gcggggatca tcagccgctc cgcgcggcgg ctgtgcgcgg cgatcgaggc gtacacgaac
     7681 gcgaacaggt acgacgcgcc gagcgcgatg aacatccgca acaggctgcg cgccgcgtcg
     7741 tagggcagcc gcgacggcga ggtatcgacc gcggtcacgt cggtcggcgt gaacgacagc
     7801 gtggtgcccc gcccgaccga gcacctcgct gatgcgcgga tgtatgagtt g
//
LOCUS       DAORNF010000040         7812 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_6887_length_7812_cov_1.592368, whole genome shotgun sequence.
ACCESSION   DAORNF010000040 DAORNF010000000
VERSION     DAORNF010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 7812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7812
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6887_length_7812_cov_1.592368"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..139)
                     /locus_tag="PLV68_02720"
     CDS             complement(<1..139)
                     /locus_tag="PLV68_02720"
                     /inference="COORDINATES: protein motif:HMM:NF012393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HRE00183.1"
                     /translation="MLNDRLLVHMPDREGERRSMGGIVIPATAQISKRLVWAEVVALG
                     QN"
     assembly_gap    347..377
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<378..>594)
                     /locus_tag="PLV68_02725"
     CDS             complement(<378..>594)
                     /locus_tag="PLV68_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRE00184.1"
                     /translation="IGLGTTIWSPLASGLLTGKYRDGVPDDSRAALPGYGWLAKRITN
                     PKQVAKVENLRPIADEIGCTLAQLALA"
     assembly_gap    595..849
                     /estimated_length=255
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<850..1194)
                     /locus_tag="PLV68_02730"
     CDS             complement(<850..1194)
                     /locus_tag="PLV68_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRE00185.1"
                     /translation="MPYRRLGHSGLQLSALSFGSWVTFDTQVNDDLAIECMQTAWDAG
                     CNFFDNAEAYAGGESEAIMGRAIEQLGWKRQSYVLSTKIFWGTDGDMVNMRNTLNRKY
                     IHHAIEGCLDRLR"
     assembly_gap    1257..1303
                     /estimated_length=47
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1304..2018)
                     /gene="hisF"
                     /locus_tag="PLV68_02735"
     CDS             complement(<1304..2018)
                     /gene="hisF"
                     /locus_tag="PLV68_02735"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HRE00186.1"
                     /translation="MRVARVIPCLDVTAGRVVKGVNFVGLRDAGDPVELAARYDREGA
                     DELVLLDITASSDGRATMVDVVARVAEQVFIPFTVGGGIRTVEDARRMLRAGADKISV
                     NTAAFEQPELISEIAAEFGAQCVVCAIDARAALGADGKPDGSYEVFLHGGRTPTGHDA
                     VAWAERAVAQGAGEILLTSMDRDGTREGFELRLTRAIADAVPVPVIASGGVGTLEHLV
                     AGIVDGGADAVLAASIFHFG"
     gene            complement(2018..2671)
                     /gene="hisH"
                     /locus_tag="PLV68_02740"
     CDS             complement(2018..2671)
                     /gene="hisH"
                     /locus_tag="PLV68_02740"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HRE00187.1"
                     /translation="MTGRPRVAVIDYGIGNLRSAEKAIQRQGGDAFLTTDPAAIAAAD
                     AVVLPGVGAFGACMRALREAGLEPAVRTAAASGRPFLGICVGMQMLFDGSEEDPAVAG
                     LGIIPGTVVWIPAGVKRPQMQWNRLVIERDDEPMLTGLGAEPWMYFVHSLHGVPADAS
                     DVVASCDYGGRLNVAFRRGSVFATQFHPEKSGEWGLALLTNFVRITASQAAEQRGEG"
     gene            complement(2697..3287)
                     /gene="hisB"
                     /locus_tag="PLV68_02745"
     CDS             complement(2697..3287)
                     /gene="hisB"
                     /locus_tag="PLV68_02745"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HRE00188.1"
                     /translation="MTRAASRSRTTKETSIEMSIDLDGHGTTAVSTGIPFYDHMLDQL
                     GRHGGFDLSITATGDIHIDTHHTVEDVAITLGETFREAVGDKVGIRRFASGLYPLDEA
                     LIEIALDLSGRPFVFWDVEMPESLPLGTPAFDPQLAEHAVSSFAMAAGITLHVTLRRG
                     RNVHHIIEATFKGLARCLRDAVRVEGGGLPSTKGLL"
     gene            complement(3284..4345)
                     /gene="hisC"
                     /locus_tag="PLV68_02750"
     CDS             complement(3284..4345)
                     /gene="hisC"
                     /locus_tag="PLV68_02750"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HRE00189.1"
                     /translation="MTRIPVRDDLSALEGYHSAQVSVDVRLNTNESPAPPPPAWRDAF
                     AAELSRVDWHRYPDRAATELRQAIADLHHVGIDQVFAANGSNEVLQTLLLTFAGPGRT
                     VATFEPTYQLHGHIARITGARVAEGERSADFTLDLTEVERVCRDASPMVTFLCSPNNP
                     TGLVEPRANLDAVLRLAPGLVVVDEAYAQFADWSAVDMVDDELPLVVTRTFSKTWSMA
                     ATRLGYLIGPAWLVAELDKVTLPYHLDVAKQIAGRLALQYLDDMEARVSGIVRERERI
                     ETAFSQLPLDYWPSGANFILFRPRTRAGRDVWQGLVERSVLVRDCSSWPRLNDCLRIT
                     VGTPAENTAFLAALTEVLA"
     gene            4453..4650
                     /locus_tag="PLV68_02755"
     CDS             4453..4650
                     /locus_tag="PLV68_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00190.1"
                     /translation="MPAPNKAEIGAVADGVETYRSRTVGLAETLIGTSHEDLLATLWE
                     AERALQNALRALQRAHKLSPA"
     assembly_gap    4663..4688
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4919..5869
                     /gene="mshD"
                     /locus_tag="PLV68_02760"
     CDS             4919..5869
                     /gene="mshD"
                     /locus_tag="PLV68_02760"
                     /EC_number="2.3.1.189"
                     /inference="COORDINATES: protein motif:HMM:TIGR03448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035447 - mycothiol synthase activity
                     [Evidence IEA];
                     GO_process: GO:0010125 - mycothiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycothiol synthase"
                     /protein_id="HRE00191.1"
                     /translation="MLVDTRLSLDAGVDAGLIREVDALLQRVEGERGYRPVSDQWWLE
                     LHHPPPGVTAVTVRDVAGALIGFALAAHHPADDGHGAWAFETVTTEAIGRAGVLDALA
                     GALVDAASAAGVHELTWTVQGPTSDHDRAAARHGLQPVRHLHQMRRALPIEMPFDFTT
                     RAFDPERDIDAWVAVNARSFAWNPEQGRWTPDDVRARMAEPWFDAAGLLVHERDGRMA
                     GFCWTKIHPAQADDPAMGEIYVVAVDPDFQGLGLGRDLTLAGLAHLAASGPRVAMLFV
                     DADNVAAIRVYERLGFVIHRTDTIFQGTIVQGTISPDPAS"
     gene            5869..6936
                     /gene="rlmN"
                     /locus_tag="PLV68_02765"
     CDS             5869..6936
                     /gene="rlmN"
                     /locus_tag="PLV68_02765"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HRE00192.1"
                     /translation="MSATLYDVDRDGLAELLADQPRYRVDQVWSGLYDQFAEPADLTN
                     LPKALRHTLAERLPTALTTVVERVSDDGDTVKYLFQLPDGGRIETVLMLYPDRVTACV
                     SSQVGCAMGCGFCATGQAGFTRHLTTGEIVEQVVRAARTAKALDRRLGNIVFMGMGEP
                     LANEAAVWPAVQRIHDDIGLGARRLTVSTVGVVPGIAKLAGWDMPVNLAVSLHAANDT
                     LRDQLVPINRRYPLGVLMDACADYLRRTGRRLSFEWALIADTNDGDHDARELADLCRS
                     LPLAAHVNIIPLNPTPGYLTRGAAPERVRAFRDRLTALGVNATVRANRGTDIDAACGQ
                     LAAGQAAKAAPVRLGRSSPQP"
     assembly_gap    7011..7125
                     /estimated_length=115
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7126..7357)
                     /locus_tag="PLV68_02770"
     CDS             complement(<7126..7357)
                     /locus_tag="PLV68_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6285 domain-containing protein"
                     /protein_id="HRE00193.1"
                     /translation="MSLHDVPTARQLVEAVREWIERDVMAATSGRVQFHARVAANVLA
                     MVERELELGADQERRHSERLAAFGGHDDAELAA"
     gene            complement(7354..>7812)
                     /locus_tag="PLV68_02775"
     CDS             complement(7354..>7812)
                     /locus_tag="PLV68_02775"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HRE00194.1"
                     /translation="TFELVFRWLDANRPTAGRRCLVHGDFRLGNLMIGPDGLRAVLDW
                     ELAHIGDPMEDLGWLCVKAWRFGGRLPVAGVGTYQQLCDAYSAAAGVETDPEVVRWWE
                     VLGTLKWGVMCIIQAHAHLSGAVSSHEMAAIGRRVCENEHDLFLALEGRW"
BASE COUNT         1154 a         2602 c         2463 g         1119 t
ORIGIN      
        1 cgttctggcc cagcgcaaca acctcggccc acaccagacg cttggagatc tgcgccgtag
       61 ccgggatgac gatgccgccc atcgagcggc gctcgccctc gcggtcgggc atgtgcacca
      121 acagccggtc gttgagcatc ttgatcggca acttctcgtt cacgcttgcc acggccggca
      181 cggtaccgcc cggcgccggg tgattgaaac cgcgccgcgt cgacgtgtcg tcaggcgacc
      241 gcggcgtcga tgcgggccag cacctcgggc gtcatcgcgt cgagcacggc gagggcaccg
      301 aagttgtcga ccacctgact gaccttgctc gctccggtga tgaccgnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnncac gccagcgcga gctgggccaa cgtgcacccg atctcgtctg
      421 cgatggggcg caggttctcg accttggcga cctgcttcgg gttggtgatc cgcttggcca
      481 gccagccgta gccgggcaac gcggcacggc tgtcgtcggg cacgccgtcg cggtacttgc
      541 cggtgagcag accgctcgcg agcgggctcc agatcgtggt gccgagaccg atctnnnnnn
      601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      841 nnnnnnnnng cggaggcggt cgagacagcc ttcgatcgca tggtggatgt acttgcggtt
      901 gagcgtgttg cgcatgttga ccatgtcgcc gtcggtgccc cagaagatct tggtcgacag
      961 cacgtagctc tgccgtttcc acccgagttg ttcgatggcg cggcccatga tcgcttcgct
     1021 ctcgccaccg gcgtaggcct cggcgttgtc gaagaagttg catccggcat cccacgcggt
     1081 ctgcatgcac tcgatcgcca agtcatcgtt gacctgggtg tcgaacgtga cccacgaccc
     1141 gaacgacagc gccgagagct gcagcccgga gtgacccagg cggcggtacg gcatggtggg
     1201 tctggtggtg ttcgtgccag tccttgcggt tgtcatggtg cagaccgtac cggacgnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncgccgaa gtggaagatc
     1321 gacgcggcca gcacggcgtc ggcgccaccg tcgacgatgc cggcgaccag gtgttccagc
     1381 gtccccaccc caccgctggc gatcaccggc accggcaccg cgtccgcgat cgcacgggtg
     1441 aggcgcagtt cgaacccttc gcgggtgccg tcgcggtcca tcgacgtgag caggatctcg
     1501 cccgcaccct gtgcgaccgc gcgttcggcc caggccaccg cgtcgtgtcc ggtcggcgtg
     1561 cgcccgccgt gcaggaacac ctcgtacgag ccgtccggct tgccgtccgc gccgagcgcg
     1621 gctcgggcgt cgatcgcgca caccacgcat tgcgcgccga actcggcggc gatctcggag
     1681 atcagctcgg gctgctcgaa cgcggccgtg ttgacgctga tcttgtcggc cccggcgcgc
     1741 agcatgcgcc gcgcgtcttc gacagtccgg atgccgccgc cgaccgtgaa ggggatgaac
     1801 acctgctcgg ccacgcgggc gaccacgtcg accatggtcg cgcgtccgtc cgacgacgcg
     1861 gtgatgtcga gcaagaccag ctcgtcggcg ccttcgcggt cgtagcgcgc ggccaactcg
     1921 accggatcgc cggcgtcgcg caggccgacg aagttgacac ccttcaccac ccgtccggcg
     1981 gtgacgtcga ggcacgggat cacgcgggcg acgcgcatca gccctccccc cgctgttcgg
     2041 ccgcctggct ggcggtgatg cggacgaagt tggtcagcag cgccaaaccc cactcgcccg
     2101 acttctccgg gtggaactgg gtggcgaaca cgctgccacg gcggaacgcc acgttgagcc
     2161 ggccgccgta atcgcaactg gccaccacat cgctggcatc ggcgggcacc ccgtgcagcg
     2221 agtggacgaa gtacatccac ggctcggcgc ccagcccggt gagcatcggt tcgtcgtcgc
     2281 gttcgatcac gagccggttc cactgcatct gcggtcgctt gacgccggcg gggatccaca
     2341 ccacggtgcc ggggatgatg cccaacccgg ccacggccgg gtcttcttcg ctgccgtcga
     2401 acagcatctg catgccgacg cagatgccca ggaacggccg ccccgatgcc gcagcggtgc
     2461 gcaccgctgg ctccaagccg gcctcgcgca gggcgcgcat gcacgcgccg aaggctccga
     2521 cacccggcag caccaccgcg tcggccgccg cgatcgcggc cggatcggtg gtgaggaacg
     2581 cgtcaccacc ctggcgttgg atcgccttct cggccgagcg cagattgccg atgccatagt
     2641 cgatcaccgc cacccgcggc cgacccgtca caacagactc ctcacgactg ggcctgtcac
     2701 aacaggccct ttgtggacgg caggccgccg ccctcgacgc gcaccgcgtc gcgcaggcag
     2761 cgggcgaggc ccttgaaggt tgcttcgatg atgtggtgga cgttgcggcc gcggcgcaac
     2821 gtgacgtgca gggtgatgcc cgccgccatc gcaaagctgc tcacggcgtg ttcggccaac
     2881 tgcgggtcga acgccggcgt acccagcggc aagctctcgg gcatctcgac gtcccagaac
     2941 acgaacggcc ggccggacag gtcgagagcg atctcgatca gcgcctcgtc cagcggatac
     3001 aacccactgg cgaagcggcg aatgccgacc ttgtcgccga ccgcctcacg aaacgtttca
     3061 cccaacgtga tcgccacgtc ctccaccgtg tggtgggtgt cgatgtggat gtcgccggtg
     3121 gcggtgatcg acaggtcgaa accgccgtgg cgaccgagtt ggtcgagcat gtggtcgtag
     3181 aacggaatgc cggtcgacac ggccgtggtg ccatggccgt cgaggtcgat cgacatctcg
     3241 atcgaggtct ccttggtggt gcgcgaacgc gacgcggcgc gggtcatgcc agaacctccg
     3301 tgagtgcggc gaggaacgct gtgttctccg cgggtgtgcc gacggtgatc cgcaagcagt
     3361 cgttgaggcg gggccagctg ctgcaatcgc gcacgagcac gctgcgctcg accaacccct
     3421 gccacacgtc tcgaccggcg cgggtgcgtg gtcggaacag gatgaagttg gcgccgctcg
     3481 gccagtagtc gagcggcagc tggctgaacg ccgtttcgat ccgctcgcgc tcgcggacga
     3541 tgccgctgac ccgagcctcc atgtcgtcca ggtactgcag cgcgagccgt ccggcgatct
     3601 gcttggccac gtcgaggtga tacggcagcg tcaccttgtc gagctcggcc accagccacg
     3661 ccgggccgat caggtagccc aggcgggtcg cggccatcga ccacgtcttg ctgaacgtgc
     3721 gggtcaccac cagtggcagc tcgtcgtcca ccatgtcgac cgcggaccag tccgcgaact
     3781 gggcgtaggc ctcgtcgacc acgaccagcc ccggagccag gcggagcacc gcgtcgagat
     3841 tggcccgtgg ctcgaccaga ccggtggggt tgttgggcga gcacaggaac gtgaccatcg
     3901 gggaagcgtc gcgacagacc cgctcgacct cggtcagatc gagcgtgaag tcggcactgc
     3961 gctcgccttc ggccaccctt gcaccggtga tgcgggcgat gtgcccgtgc agctggtacg
     4021 tcggttcgaa cgtggccacg gtgcggcccg gtccggcgaa ggtcagcaac agcgtctgca
     4081 gcacctcgtt cgacccgttg gccgcgaaca cctgatcgat ccccacgtga tggaggtcgg
     4141 cgatcgcctg gcgcagctcg gtggcggctc gatcggggta ccggtgccag tcgacacggc
     4201 tcagctcggc ggcgaaggcg tcacgccacg ccggaggcgg cggggcaggc gactcgttgg
     4261 tgttgagccg gacgtcgacg ctgacctgtg cagagtggta gccctcgagc gcggagaggt
     4321 cgtcgcggac ggggatgcgg gtcacgccga cgaacgttac cgaggcaccc ccgcgcccag
     4381 aaatcgattg caccagcagc cgatcccgac gatcgccgcc gcaccgccac ggggccgcgc
     4441 cagactgagc cgatgcccgc acccaacaag gccgagatcg gtgccgtcgc cgacggtgtc
     4501 gagacctacc gttcgcgtac cgtcggcctg gccgagaccc tcatcggcac cagccacgaa
     4561 gacctgctgg ccaccctgtg ggaggccgag cgagcgttac agaacgcgct gcgtgcgtta
     4621 cagcgcgctc acaagctctc gccggcctga gctgacagcg tgnnnnnnnn nnnnnnnnnn
     4681 nnnnnnnncc ccttgcgggg ccgtcgcgct tgcgatgcca ggcggcggat cccggcatcg
     4741 cccgcaaacc aggtggcggg aacctggctg gtggcgccag tgtacaggac aaccgcaccg
     4801 gcgcaatcgg caggtcagca cccggtgcag gcacggtgcg cacgccgcca cggcgatgtg
     4861 aagagccggg atgtgaggag ccggtctcgg ttcgggcgcc agcgatagcg tgggcacggt
     4921 gctggtcgac acccgtctct cccttgacgc tggcgtcgat gctggtttga tccgtgaggt
     4981 cgacgcgctg ttgcaacggg tcgagggcga gcgcggctat cggcccgtgt ccgaccagtg
     5041 gtggctggaa ctgcaccacc cgccgccagg tgtcacggcg gtcaccgtcc gcgacgttgc
     5101 gggtgcgttg atcgggttcg ccctggcggc tcaccaccca gccgatgatg gtcacggagc
     5161 gtgggcgttc gagacggtga cgaccgaggc gatcggccgg gccggggtgc tcgatgcgct
     5221 cgccggcgcg ctggtcgatg ccgccagcgc agccggagtc cacgaactca cctggaccgt
     5281 gcaaggcccg accagcgacc acgatcgcgc cgccgcgcgg catggtctcc agccggtgcg
     5341 gcatctgcac cagatgcgtc gggcgctacc gatcgagatg ccgttcgact tcaccacccg
     5401 ggcattcgac cccgaacgtg acatcgacgc gtgggtcgct gtcaacgcgc gatccttcgc
     5461 gtggaaccca gagcaggggc gctggacgcc agacgacgtt cgcgcccgca tggccgagcc
     5521 gtggttcgat gcggcgggtc tgctcgtaca cgaacgtgac gggcggatgg ccgggttctg
     5581 ctggaccaag attcatccgg cacaggccga cgatcccgcg atgggcgaga tctacgtggt
     5641 cgcggtcgac cccgacttcc aaggactggg cctcggccgc gatctgaccc tggccggtct
     5701 cgcccacctg gccgccagtg gtccccgcgt cgcgatgctg ttcgtcgacg ccgacaacgt
     5761 tgccgcgatc cgcgtctacg agcgactcgg tttcgtgatc caccgcaccg atacgatctt
     5821 tcagggcacg atcgtccagg gcacgatctc gccagacccg gcgtcgtgat gagcgccacc
     5881 ctgtacgacg tcgaccgcga tggcctcgcc gaactgctcg ccgatcagcc ccgctaccgg
     5941 gtcgaccagg tgtggtccgg cctctacgac cagttcgccg aaccggccga cctcaccaac
     6001 ctgcccaagg cactgcgtca cacccttgcc gagcgactcc ccaccgccct gaccaccgtg
     6061 gtcgaacggg tcagcgacga cggcgacacg gtgaagtacc tgttccaact gcccgacggc
     6121 ggtcgcatcg agacggtgct gatgctctac cccgatcgcg tcacagcctg cgtcagcagc
     6181 caagtgggtt gcgccatggg gtgcgggttc tgcgccaccg gccaggccgg cttcacccgt
     6241 cacctcacca ccggagagat cgtcgaacag gtggtgcgcg cggcccgtac cgccaaggcg
     6301 ttggatcgac ggctcggcaa catcgtgttc atgggtatgg gcgagccgct cgccaacgag
     6361 gccgcagtct ggcccgcggt gcagcgcatc cacgacgaca tcggcctggg cgcgcggcgc
     6421 ctcaccgtgt cgaccgtcgg cgtggtgccc ggcatcgcca agctggccgg ctgggacatg
     6481 ccagtcaacc tggccgtgtc cctgcacgca gccaacgaca ccctgcgcga ccagttggtg
     6541 ccgatcaacc gccgttaccc gctcggggtg ctcatggacg cctgcgccga ctacctgcgt
     6601 cgcaccggcc gccgattgag cttcgagtgg gcgctgatcg ccgataccaa cgacggcgac
     6661 cacgacgccc gcgagctggc cgatctgtgt cgctcgctcc cgctcgcggc ccacgtcaac
     6721 atcatccccc tcaacccgac accgggttat ctcacccgcg gtgcggcacc cgaacgggtc
     6781 cgcgcctttc gtgaccggct caccgcgctg ggggtcaacg ccaccgtgcg ggccaaccgc
     6841 ggcaccgaca tcgacgcggc ctgcgggcag ttggcggccg ggcaagcggc caaggcagcg
     6901 cccgtcaggc tgggccggag ctctccccag ccctgagcgg ctcgccgagc gacccgtcac
     6961 cgctcgaccc gtcaccgagc gacccgtcac cgctcgatgc ggcaccgagc nnnnnnnnnn
     7021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnccgca gccagctcgg
     7141 cgtcgtcgtg tcctccgaac gcggccagcc gttcgctgtg gcgtcgttcc tggtcggcac
     7201 cgagctcgag ctcgcgctcg accatcgcca agacgttggc cgcgacccgg gcatggaact
     7261 ggacgcgtcc cgacgtggcc gccatgacgt cgcgttcgat ccattcccgc acagcctcga
     7321 ccagttggcg agcggtcggt acgtcgtgca ggctcaccag cgtccctcca gggccaggaa
     7381 cagatcgtgt tcgttctcgc agacccgccg gccgatggcg gccatctcgt ggctggacac
     7441 cgcgcccgac aggtgagcgt gggcctggat gatgcacatc acgccccact tgagggtgcc
     7501 gagcacctcc caccaacgca ccacctcggg gtcggtttcc acgcctgcgg cggcactgta
     7561 ggcatcgcac agctgctgat acgtgccgac gccagcaacc ggcaagcggc cgccgaagcg
     7621 ccacgccttg acgcacagcc agccgagatc ctccatcgga tcgccgatgt gagccagctc
     7681 ccagtcgagc acggcgcgca gaccgtcggg accgatcatc aggttgccca accggaagtc
     7741 tccgtgcacc aagcaccgcc gtccggccgt aggacggttg gcgtcgagcc agcggaacac
     7801 gagctcgaag gt
//
LOCUS       DAORNF010000041         7779 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_6930_length_7779_cov_1.643708, whole genome shotgun sequence.
ACCESSION   DAORNF010000041 DAORNF010000000
VERSION     DAORNF010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 7779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7779
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6930_length_7779_cov_1.643708"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    252..261
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <262..869
                     /locus_tag="PLV68_02780"
     CDS             <262..869
                     /locus_tag="PLV68_02780"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HRE00195.1"
                     /translation="FVAGCASFVGAPSLSSSPMPNMAITTLGVTWIGLPGAFAGLILS
                     ANVSGAGVSSFVGTDTLLVMAIAVVINDIGALFVGSGIGKTPLRGWISPNKSVEGFIG
                     GAVLTVIAMVIYNILGKSASWNGINVVLLGIAVSIAAPLGDLTESMFKRNLDIKDFGS
                     IIKGHGGVLDRFDGFLFALPTAYYLLVVLEPWVPAAAAFKP"
     assembly_gap    1050..1133
                     /estimated_length=84
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1134..>1427
                     /locus_tag="PLV68_02785"
     CDS             <1134..>1427
                     /locus_tag="PLV68_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HRE00196.1"
                     /translation="VVAVADPQRRAEVAAALPFADVVADMADLTNGADTVVNAVVGFA
                     GLAVTVATLRAGLRLALANKESLIAAGPVVQPLRATPGAELIPVDSEHCALHP"
     assembly_gap    1428..1499
                     /estimated_length=72
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1500..>1803
                     /locus_tag="PLV68_02790"
     CDS             <1500..>1803
                     /locus_tag="PLV68_02790"
                     /inference="COORDINATES: protein motif:HMM:NF020025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HRE00197.1"
                     /translation="LADVSVDQALAHPTWSMGPKITIDSSTLMNKGLEVIEAHELFGV
                     GYDDIDVVVHPQSVVHSMAEFTDGATIAQLSLPDMRLPIAYALAYPDRGRVPFGRI"
     assembly_gap    1804..1857
                     /estimated_length=54
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1858..2100
                     /locus_tag="PLV68_02795"
     CDS             <1858..2100
                     /locus_tag="PLV68_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HRE00198.1"
                     /translation="DLAYAAGRQGGSAPAWLSAANEEAVDAFLNHRIRWNQIAEVCDA
                     VLQRHHQPGEIAQVGDIIAADATARRIAHEELSSLS"
     gene            2111..3361
                     /locus_tag="PLV68_02800"
     CDS             2111..3361
                     /locus_tag="PLV68_02800"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HRE00199.1"
                     /translation="MSTTFDRIRGEMVAGGALGEDDPTVGGAQAALRLTVLAALFVLL
                     LWASPWAFVFVIGLLISIFLHELGHFVSARKSGMKVTQFFIGMGPRLWSFRRGEVEYG
                     VRALPIGAFVRIVGMNNLDPAEPADQARAYMNQTYPRRMITICAGVLMNLALAVVLLF
                     GVYATYGERGETGRVGVSGVAPDTAAAAAGLQRGDIYVSIDGHAISSYVDVSAAISAH
                     QPGDTIEVVVERDGRTEPLQATLGEHPNPEVTTAFLGVTSGDELGWHKQSIASAAVGS
                     VTDLGDAARRIVASLPIVFNPVNVFEHVTGERTDPNTQPVTVVGVSRLSAIIGSESGL
                     GGVLMTLGGINVMLALLNMVPLLPFDGGHAAMATYERLRSRKGQPQYHADANKLVPVA
                     MAVLSLVVFVMLAGLWLDIVRPVR"
     gene            3378..4598
                     /gene="ispG"
                     /locus_tag="PLV68_02805"
                     /gene_synonym="gcpE"
     CDS             3378..4598
                     /gene="ispG"
                     /locus_tag="PLV68_02805"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HRE00200.1"
                     /translation="MSSSFPRRETRTINVGKVAVGGDAPISVQSMTITKTADVEATLQ
                     QIYALAAAGCDIVRCTCNEEAAAVGLAQIVPRSPIPIIADIHHQYKMALAALEAGVHG
                     LRLNPGNIRRAEHIKAVASEARDRQVPIRIGVNGGSLDPALYDKWGGVRPEAMVESAL
                     QELAYFQEVDFDLVKISVKASSVTLMVEAYRQLSEVTDVPLHLGVTEAGPPPAGLVKA
                     TAGIATLLMEGIGDTIRYSLTADPVEEARAGRQLLEALGLRERKNVDLIACPSCGRAE
                     VDVIDVAKRAMDAFGDRKIPLQVAVMGCVVNGPGEAREADLGIAAGNKRGHLFVKGRN
                     VAVVPEAEMVDALVDWAEFIHEHGTEAALARVDTTKAEREAARDRAALLDDKGADANH
                     AAERIVEIRKTISG"
     gene            complement(4669..5730)
                     /gene="manA"
                     /locus_tag="PLV68_02810"
     CDS             complement(4669..5730)
                     /gene="manA"
                     /locus_tag="PLV68_02810"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HRE00201.1"
                     /translation="MIGTVRHYDWGDHEVIPRLLGVEPDGRPHAELWFGTHPAAPSML
                     GDGDTLESATGPLPYLVKVLAAGQPLSLQVHPSIAQAQDGCAREDAAGIPLDAPQRTY
                     RDPFHKPEILVAMTPFEALCGIAPIEQTDALLAALGEPAASIRATIQRGGVDAAIGMI
                     LHDRPSIDHLVAAAAHHDDPRCVWLTTLVQQHPGDPTAALVLLLNYVALTPGEAVYLG
                     AGNLHAYLRGAGVEVMAMSDNVVRAGLTHKHIDADELLRVLDTTPLADPVVRPVAAPD
                     GGLVYPVPVTDFRVASYTIDGSVDLIADGAELYLCTNGETTEVGHGECALASDGEHVE
                     LVGTATVFRVGTSRVSVNT"
     gene            complement(5921..6580)
                     /gene="nucS"
                     /locus_tag="PLV68_02815"
     CDS             complement(5921..6580)
                     /gene="nucS"
                     /locus_tag="PLV68_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease NucS"
                     /protein_id="HRE00202.1"
                     /translation="MRLVIARCTVDYAGRLSAHLPEAVRLIMVKADGCVAIHADGGSY
                     KPLNWMNAPNTLTDNGDHWVVVNPKGEQLTINLVEVLHDAAHDLGDDPGLQKDGVEAH
                     LQELLAAAPHSIEQGLTLVRREYPTAIGPIDLVCRDASGTVVAIEVKRRGEIDGVEQL
                     TRYIDRLHLDSSLGRVRGVFVAQQVKPQARVLAESRGFRWVEVDYDELRGLRPDELRL
                     F"
     gene            complement(6600..>7212)
                     /locus_tag="PLV68_02820"
     CDS             complement(6600..>7212)
                     /locus_tag="PLV68_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HRE00203.1"
                     /translation="DLHGGNLFVQHDGTIALLDFGIVGRLTHPRRLAFLRLMLGSITN
                     DVTGQLAALRDLGALPLDTDLAAVVSDLGLDKPPIDPTTLTGEEMVREVQRVVKALLG
                     YGAKLPKELMLYVKNLVFLDGAISRLAPDLDILAEITHVSMQFTARHGERLGQELGMQ
                     PIALDIDLTGVKANFGVDASTEALTYRDLQKRRELIQKRMRER"
     assembly_gap    7213..7234
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7235..>7779)
                     /locus_tag="PLV68_02825"
     CDS             complement(<7235..>7779)
                     /locus_tag="PLV68_02825"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HRE00204.1"
                     /translation="VPAESWRDVRRTVEADLGAPLSDVFSHFATTPLAAASIAQVHAA
                     TLRSGEEVVVKVQRPQVASLVRRDLRVMAWLAPHLVGRIPVAALANPPALVELFAETI
                     VEELDFRLEAANMLDMARMIRRLDQVGFVVPRPHPHLVTRRVMVMERLRGFNFDDVAG
                     MQNAGIDTEAIVRAGMIAFME"
BASE COUNT         1175 a         2488 c         2633 g         1241 t
ORIGIN      
        1 cggtgccggt ctggccgcgc tgttcatcgt cgcgatcaag atcggcgcgg tggcgacatt
       61 ggcggtcgtc gtcgccgtga tcaccctggc ggcgatcgag ttcttcgcca aggtcaccga
      121 gaagggctat cagccggcga gcacgatcgg catcagcgcg cgttcgacca gcgtgccgcg
      181 cttgaccgcg gtggcgatgc ccaccgcgat gcccatcatg gtcgggaacc ggtcggccag
      241 caggaatccc annnnnnnnn ncgttcgtgg ccggatgtgc cagcttcgtc ggcgccccca
      301 gcctgtcgtc cagccccatg cccaacatgg cgatcaccac cttgggtgtc acctggatcg
      361 gtctgccggg cgcgttcgcc gggttgatcc tcagcgccaa cgtctcgggt gcgggtgtgt
      421 cgagctttgt cggcaccgac accctgctgg tgatggcgat cgcggtggtc atcaacgaca
      481 tcggcgcgtt gttcgtcggc tcgggcatcg gcaagacccc gctgcgcggt tggatcagcc
      541 ccaacaagtc ggtcgagggg ttcatcggcg gcgccgtgct gacggtgatc gcgatggtca
      601 tctacaacat cctcggcaag agcgcgtcgt ggaacggcat caacgtggtg ctgctcggca
      661 tcgccgtgtc gatcgccgcg ccgctcggtg acctgaccga gagcatgttc aagcgcaatc
      721 tcgacatcaa ggacttcggc agcatcatca agggccacgg cggggtgctc gatcgcttcg
      781 acgggttcct gttcgcgctg cccaccgcgt actacctgct ggtcgtgctc gaaccgtggg
      841 tgcccgccgc ggcggcgttc aagccctgac ccggtgacca caccggtgag cccagatccg
      901 gtgagcccag tcgcgggcgt cacgaagcgg ccgccgttgc tgcgtgtcgt ttcactgcca
      961 gactggcagc catgacagcg gttcgcgtcg ccatcgccgg ctcgaccggc tccggctcga
     1021 tgctgcccga actgaggacg atttcctcgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnaggtggt
     1141 cgccgtcgcc gatccgcagc ggcgggccga ggtcgcggcg gcgttgccct tcgcggacgt
     1201 ggtcgccgac atggccgacc tgaccaacgg cgccgacacg gtggtcaacg cggtcgtcgg
     1261 cttcgccggg ctggcggtca cggtcgccac gctgcgggcg ggtctgcgcc tggcgctggc
     1321 caacaaggag agcctcatcg ccgccggccc cgtggtgcag ccgttgcggg cgacgccggg
     1381 cgccgagctg atcccggtcg attccgagca ctgtgccctg cacccatnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna
     1501 gttggccgac gtcagcgtcg accaagcgtt ggcccacccc acgtggtcga tgggcccgaa
     1561 gatcacgatc gattcgtcga cgctgatgaa caagggtctc gaggtgatcg aggcccacga
     1621 gctgttcggc gtcggctacg acgacatcga cgtcgtggtc cacccccagt cggtggtcca
     1681 ctcgatggcc gagttcaccg atggggccac catcgcccag ctcagcctgc ccgacatgag
     1741 gctgccgatc gcctacgcct tggcgtatcc cgaccgcggc cgggtgccgt tcgggcgcat
     1801 cgannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngat
     1861 ctggcctacg ccgcgggacg ccagggcgga tcggctccgg cctggctcag cgcggccaac
     1921 gaagaggcgg tcgacgcctt cttgaaccat cgcatccgct ggaaccagat cgccgaggtg
     1981 tgcgacgcgg tgctccagcg ccatcaccag ccgggcgaga tcgcgcaggt cggcgacatc
     2041 atcgccgccg atgccactgc ccggcgcatc gcccacgaag aattgagttc cctatcatga
     2101 gcgtcatgtc atgagcacca cgttcgacag aatccgtggc gagatggtcg ccggtggcgc
     2161 gctgggggag gacgacccga cggtgggtgg cgcccaggcg gcgctgaggt tgaccgtgct
     2221 ggcagcgctg ttcgtcctgc tgctctgggc cagcccgtgg gcgttcgtgt tcgtgatcgg
     2281 gctgttgatc tcgatcttcc tccacgagtt gggacacttc gtgtcggccc gcaagtcggg
     2341 catgaaggtc acgcagttct tcatcgggat ggggccacgg ctgtggagct tccgccgcgg
     2401 cgaggtcgag tacggcgtgc gcgccctgcc gatcggcgcc ttcgtgcgca tcgtcggtat
     2461 gaacaacctc gacccggccg agcccgccga ccaagcgcgg gcatacatga accagaccta
     2521 cccacggcgg atgatcacga tctgtgccgg cgtgctgatg aacctggctc tggcggtcgt
     2581 gttgctgttc ggcgtctatg ccacctacgg cgagcggggc gagaccggac gagtaggagt
     2641 gtcgggcgtc gcgcccgaca ctgccgctgc cgcagctggc ctccagcgcg gcgacatcta
     2701 cgtgtcgatc gacggccacg ccatctcgtc gtacgtcgat gtgagcgcgg cgatctcggc
     2761 ccaccagccg ggtgacacga tcgaggtcgt cgtcgagcgt gacggtcgaa ccgaaccgtt
     2821 gcaggcgacg ctgggagaac atcccaaccc cgaggtgacg acggcgtttc tcggcgtgac
     2881 ctccggggac gagttgggat ggcacaagca gtcgatcgcc tcggccgcgg tcggcagcgt
     2941 caccgacctt ggtgatgctg cgcggcggat cgtcgccagc ttgccgatcg tgttcaaccc
     3001 ggtcaacgtg ttcgagcacg tcaccggtga acggaccgac cccaacacgc agccggtcac
     3061 cgtggtcggt gtcagccggt tgagcgccat catcggttcc gagagcggtc tcggcggggt
     3121 gctgatgacg ctcggaggca tcaacgtcat gctcgctctg ttgaacatgg tgccgctgct
     3181 gccgttcgac ggcgggcatg cggcgatggc cacctacgag cgtctccgct cgcgcaaggg
     3241 gcagccgcag taccacgccg acgccaacaa gctggtgccg gtggcgatgg cagtgctgtc
     3301 gctggtcgtg ttcgtgatgt tggccggcct gtggctcgac atcgtccggc cggtgaggtg
     3361 atcgcgccat gcaggggatg agttcgtcct ttccacggcg tgagacccgc acgatcaacg
     3421 tcggcaaggt tgccgtcggt ggcgacgccc cgatcagcgt gcaatcgatg acgatcacca
     3481 agaccgccga cgtcgaagcc acgctgcagc agatctacgc ccttgccgcg gcgggctgcg
     3541 acatcgttcg ttgcacatgc aacgaagagg cagccgcagt cgggctggcc cagatcgtgc
     3601 cccgctcgcc catcccgatc atcgccgaca tccaccatca gtacaagatg gccctcgccg
     3661 cgctcgaggc cggcgtccac ggcttgcgcc tcaacccggg caacatccgt cgggccgagc
     3721 acatcaaagc cgtcgcctcc gaagcgcgcg accgtcaggt gccgatccgc atcggtgtca
     3781 acggcggttc gctcgacccg gccttgtacg acaagtgggg cggggtgcgc cccgaagcga
     3841 tggtcgagtc ggcgctacag gagctggcct acttccaaga ggtcgacttc gacctggtca
     3901 agatcagcgt caaggccagc agcgtcacgc tgatggtcga ggcctatcgc caactcagcg
     3961 aggtcaccga cgtgccgttg cacctcggcg tcaccgaggc cggtccgccc ccggccgggc
     4021 tggtcaaggc caccgcgggt atcgccacct tgttgatgga aggcatcggc gacaccattc
     4081 gctacagcct caccgcggac ccggtcgaag aggcgcgggc cggtcgtcag ttgctcgaag
     4141 cgctgggtct gcgcgagcgc aagaatgtcg acctgatcgc ctgcccgagc tgcgggcgag
     4201 ccgaggtcga cgtgatcgac gtcgccaagc gggccatgga cgcgttcggc gaccggaaga
     4261 tccccctgca ggtcgcggtg atgggctgtg tcgtcaacgg tccgggcgaa gcgcgcgagg
     4321 ccgatctcgg catcgcggcc ggcaacaagc gcggtcacct gttcgtcaag ggacgcaacg
     4381 tcgctgtggt gcccgaagcc gagatggtcg acgcgttggt cgactgggcc gagttcatcc
     4441 acgagcacgg taccgaggcc gcgttggccc gcgtcgacac caccaaggcc gagcgcgaag
     4501 cagcccgcga ccgggccgcg ctgttggacg acaagggtgc cgacgccaac catgccgccg
     4561 agcgcatcgt cgagatccgc aagacgatct ccggctgatc cacgacacgt ctcgacgccg
     4621 cgtcccgctg agcgtcagca cgctgcggcg acccgcgccg agtcggctct atgtgttgac
     4681 gctgacgcga gaggtgccga cacggaacac ggtggcggtg ccgaccagtt cgacgtgttc
     4741 gccatcggac gccagcgcgc actcgccgtg gccgacttcg gtcgtctcgc cgttggtgca
     4801 caggtacagc tcggccccat cggcaatcag gtcgacgctg ccgtcgatgg tgtagctggc
     4861 gacgcggaag tcggtgaccg gcacggggta caccaaaccg ccgtcgggcg ctgcgaccgg
     4921 gcgcaccacg gggtcggcca gcggggtcgt gtcgaggaca cgcagcagtt cgtcggcgtc
     4981 gatgtgcttg tgggtcagac cggcgcggac cacgttgtcg gacatggcca tgacttcgac
     5041 gcccgcgccg cgcaggtacg catgcaggtt gcccgcaccg aggtacaccg cctcgcccgg
     5101 ggtgagagcg acgtagttca gcagcagcac gagcgccgcg gtcgggtcac cagggtgctg
     5161 ctgcaccagc gtggtcaacc acacacagcg cgggtcgtcg tggtgcgcgg ccgcagcgac
     5221 caggtggtcg atcgaaggac gatcgtgcag gatcatgccg atcgccgcgt cgacaccacc
     5281 gcgctggatc gtggcgcgga tcgacgccgc gggttcgccc agcgcggcta gcagggcgtc
     5341 tgtctgctcg atcggggcga tgccgcacag cgcttcgaac ggcgtcatcg cgaccaggat
     5401 ctcgggtttg tggaacgggt cgcgatatgt gcgctggggt gcgtcgagtg ggatgcccgc
     5461 cgcgtcttca cgagcacagc catcctgggc ttgggctatc gaggggtgca cctgcagtga
     5521 gagcggttgg ccggccgcga gcactttgac caggtatggc agcggcccgg tggccgactc
     5581 gagcgtgtcg ccgtcgccca gcatcgacgg cgctgcgggg tgggtgccga accacagttc
     5641 ggcgtgcggg cgcccgtcgg gctcgacccc cagcaatcgc gggatgacct cgtgatcgcc
     5701 ccagtcgtag tggcgaacgg taccgatcac gcgttgcacg gtcggccagg ttagacggtg
     5761 ccccaggccg gggtgatggc gggacggttg cgctggtggc gagatcctgc ggctacggcc
     5821 aacaccttcg aacggtgtgg tgaccagatc gtgtgggtcg ggctgctcgg cgtcgacgat
     5881 ccgggcgatg ctggccccgg cgttgtgccc ggtgcgtcga tcagaacagg cgcagctcgt
     5941 cggggcgcag gccgcggagt tcgtcgtagt cgacctcgac ccagcggaac ccgcgtgact
     6001 cggccaacac ccgtgcctgc ggcttgacct gctgggcgac gaacacgccg cgcacccgac
     6061 cgagcgacga gtcgaggtgg agccggtcga tgtagcgggt cagctgctcg acgccgtcga
     6121 tctcaccgcg gcgtttgacc tcgatcgcga cgacggtgcc ggacgcgtcg cgacagacca
     6181 gatcgatcgg cccgatcgcg gtcgggtact cgcggcgcac cagggtgagc ccttgctcga
     6241 tcgagtgcgg ggcagcggcg agcagctctt ggaggtgggc ctcgacaccg tctttttgca
     6301 gaccggggtc gtcgcccaag tcgtgggccg cgtcgtgcag cacctcgacc aggttgatcg
     6361 tgagctgttc gcccttgggg ttgaccacca cccagtggtc gccgttgtcg gtcaaggtgt
     6421 tcggcgcgtt catccagttg agcggcttgt acgagccgcc gtcggcgtgg atggcgacgc
     6481 acccgtcggc tttgaccatg atcaggcgca ccgcttcggg caaatgggct gagaggcgcc
     6541 cggcatagtc gacggtgcag cgggcgatca cgaggcgcat cgctgcgcca gggtagctgt
     6601 caccgttcgc gcatgcgctt ctggatcaac tcgcgccgct tctgcaggtc gcggtaggtg
     6661 agggcctcgg tcgacgcatc gacaccgaag ttggccttga cgccggtgag gtcgatgtcg
     6721 agcgcgatcg gttgcatgcc gagctcttgg ccaaggcgtt cgccgtggcg ggccgtgaac
     6781 tgcatcgaca cgtgggtgat ctcggccagg atgtcgaggt cgggtgcgag gcgggagatc
     6841 gccccgtcga ggaacaccag gttcttgacg tacagcatca gttccttggg cagcttggcg
     6901 ccatagccga gcagcgcctt gaccacccgc tggacctcgc ggaccatctc ctcgccggtc
     6961 agcgtggtcg ggtcgatcgg cggcttgtcg agccccagat cgctgaccac cgcggccaga
     7021 tcggtgtcga gcggcagcgc acccaggtca cgcagggcgg caagttggcc ggtgacgtcg
     7081 ttggtgatcg agccgagcat cagccgcaag aacgcaaggc ggcgagggtg ggtcagacgc
     7141 ccgacgatgc cgaagtcgag cagcgcgatg gtgccatcgt gctgcacgaa caggttgccg
     7201 ccgtgcaggt cgnnnnnnnn nnnnnnnnnn nnnnttccat gaacgcgatc atccccgcgc
     7261 gcacgatcgc ttcggtgtcg atgcccgcgt tctgcatgcc agccacgtcg tcgaagttga
     7321 acccgcgcag gcgctccatc accatcaccc gccgcgtcac cagatgggga tgcggacggg
     7381 gtacgacgaa gcccacctgg tcgaggcgac ggatcatccg cgccatgtcg agcatgttgg
     7441 ccgcttcgag gcgaaagtcg agctcttcga cgatcgtctc ggcgaatagc tcgaccagcg
     7501 ctggcggatt ggccaaggcg gcgaccggga tgcggccgac caggtgcggc gccagccacg
     7561 ccatcacgcg caggtcgcgg cgcaccagcg acgccacctg cgggcgctgt accttgacga
     7621 cgacctcctc gcccgagcgc agcgtggccg cgtgcacctg ggcgatcgac gccgcggcga
     7681 gaggcgtcgt ggcgaagtgg ctgaagacgt cgctcagcgg cgcgccgagg tcggcttcga
     7741 ccgtgcggcg cacgtcgcgc cacgactcgg ccggaacct
//
LOCUS       DAORNF010000042         7760 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_6959_length_7760_cov_2.123556, whole genome shotgun sequence.
ACCESSION   DAORNF010000042 DAORNF010000000
VERSION     DAORNF010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 7760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7760
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6959_length_7760_cov_2.123556"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    214..255
                     /estimated_length=42
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<256..747)
                     /locus_tag="PLV68_02830"
     CDS             complement(<256..747)
                     /locus_tag="PLV68_02830"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HRE00205.1"
                     /translation="MAFGQQSGPPASAKQIAYLQALLDAAGYTFATARHQYGLTQRQA
                     RGKFSVSEASALIDRLVEATSAYLMAQVEAGAEALQIFDTWAGSVPAGLFERAVTGPT
                     ARIVAAVKANHPDVPIIGFPRGAGGHLGPYARATGVDGIGVDHMCDIAFAATVVPPNV
                     ALQG"
     gene            complement(755..>2969)
                     /gene="leuS"
                     /locus_tag="PLV68_02835"
     CDS             complement(755..>2969)
                     /gene="leuS"
                     /locus_tag="PLV68_02835"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HRE00206.1"
                     /translation="PAENAAKKRGVDPAEWTYANIEQQAASIRRLGYDFDWSTRLHTC
                     DPEYYHWTQWLFLKLFDAGLAYRKDGMVNWCPGCQTVLANEQVVNGLCERSDDVVVRR
                     PLIQWYFRTTRYAQELLDDLATIDWPERVKTMQRNWIGRSEGAQLSFATADGGDDVAV
                     YTTRPDTIFGATYFVFAPEHPVVAKRMADDPAYAEFLTEVSKRSEIERQATLGPGQVV
                     KRGLRLNFDMVNPFTGEHIPAYAADYVLMDYGTGAIMAVPFGDQRDFEFAKANGLEIR
                     AILRPDSADPAALAAADPATMTQAFTDYGIVVNSGDYDGLTTADAKNAIMDDLEAKGW
                     GARMVNFRLRDWLISRQRSWGPPIPIIHCEACGPQGVPEADLPVRLPADLDWSVNGSP
                     LALHPTWKHVACPSCGAPAVRDTDTMDTFVDSSWYFFRYLSPDDSTQAWPVDLASKLL
                     PVDQYTGGVEHAILHLLYARFAVKAMRDLGYVQASEPFNALLNQGQVILDGAAMSKSR
                     GNLVTPGEVVGNYGADTARATILFASAPEDDIDWADVSPSGMNKWLNRVWRQALTEGP
                     ASVADAGSPEAIDTLRRAVHARIAAISDDFTRKKYNTAIAKLMEVANAAREAAAAGVH
                     GTPMREAIDALLTMLAPVCPFITDELWQRIGREGSVHQQRWPVADPALLVKSETTIVA
                     QVNGKIRARLTVAVDLDAAEIERIARAEAPVAAALEGATVKNVFVVPNRLINFVV"
     assembly_gap    2970..3137
                     /estimated_length=168
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3463..>7470
                     /locus_tag="PLV68_02840"
     CDS             3463..>7470
                     /locus_tag="PLV68_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00207.1"
                     /translation="MLLALVGVLVSSVAVTNAMIANAAPTSPPPGPVVAPGAPSRACV
                     NGEEGLSSSYAYGYYWSEVGRRWVQQPMCAVLWSGITLMASQKGAPGDTFTFNVTSLT
                     NTTVYGPPRNAMSWSHPGTVVAGCTGADFSCTFKLGTVGQNLAEYATSLVSVSLPYFY
                     RDDYPQIGCTPETPCGAYTTVAYSWVTLTPNWGQPTADFDVDVTGDRIDVVAISADPV
                     AKREMTHVWTMDSEGSAEGVTATHRFARSGVVDVTLVSTTDDGRTATITKQVEIEQSP
                     VTMELDPDFTQLAPGKTSTVDLVLTSTDPDASTTVTLDPALVANNGLFTITPPASAAS
                     PITVPPNGVVRLSFGVEAGDDLGSDTFRSAGVVESASGKQWNIQATAPTLVAEHFVNA
                     VLTFDPDEVTLEEDGDGPAPRTINLHLDITNDTDQTLTDLVLALGNPGWQFPASFITA
                     TPGDVVHLADTENTDREFTLGDLAPHASTTLDIPVVVTDDGRIEWGGLIRLTPEDGRE
                     RTEAATKGKLTSEPKYLARFEFIDWPSGHRVNAGLDQNVAEPWNTDQIVKAGQAFGVL
                     GKVKNLTTDQMVMADLPTTALDHITSAVFRGSADEGAAPTTDELLTAGLPYRQTLEPG
                     QTDVVSAYPSTVEDESFGPGETFSSRFTVAPAASARNEDGSYPDAVPAKVLADDEGLT
                     ASAATMTPPASGSPNANVGYLGRFSIGYVEGGIRFVKGSAASIAWLLTKAPQLPGMSV
                     AAGTQFLKVASRLTGTMVAVGIELAEMTPAQRSALVDQVTAEAVNFLVMKAPDTSAAV
                     FEAVHNAVDQAIVKLMTLAMDDPGQFWESMGSFTASAAGEISIGVLTDSVVAKVMTKL
                     RVGEGLAKAERFLADQAAAVGLKLEKGLAGIRAGTILTDELLAKAMGITRNQIDQMTV
                     IAKNFGVNIYARSRGSRAIELIENLTATMKPYFIKPKSVNDIDQLFLGFERLQPDVVA
                     LRKPPTWAELSEKMAAYRTEAGEALTAGEQELVISRWKTRNKEWWGQSGDIGIDIDPT
                     DHASYLKSERYKLESKNGKLVALTLETEGNLEDQIVKAGDFATSKSTFQLAVSPDDAD
                     ALIPMLRAQGESNFKVVVGDVDLVAITYPDGTVPPLDDLIKIYRELQKEPLNMQHSVT
                     LTWTNHSPAIKMLGDHVGPNAEPLLELAVSGTRRAVKFDPAMSFADGAYYKKGAQFLH
                     MAGGEINPPGAYATAAALVWQVTEPVVNLYSPPGAYGADMKRSAPPTRVGEDGTEVLV
                     DGVWQPGGASAQQAHQAEAIRYAETVSSAAAEMPEPPLPSVVGVQSAIDLEVAAGATT
                     LPVLGRAALYPFGGAA"
     assembly_gap    7471..7484
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1252 a         2577 c         2588 g         1119 t
ORIGIN      
        1 gattggtcag aaacggctgg accgcgtcca gcgtgtccgg cccgcccaga ttgaacagga
       61 caacgcccag acgcatcagt tggcgcctcg taccactgcg accagccggg ccacgttctc
      121 cggcggggtc gggggggtta cgccatggcc gagattgaag atgaagggcc gccccttcat
      181 ggcggtgacg atccggcgcg cctcgtcttc catnnnnnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnntcctt gcagggctac atttggcggt acgacagtgg ccgcgaaggc
      301 gatgtcgcac atatgatcga cgccgatccc gtcgacgccg gtcgcccgtg cataggggcc
      361 aagatgtccg ccggcgccgc gcggaaatcc gatgatcggc acatcggggt gattggcctt
      421 caccgcggcg acgatgcgcg ccgtcggacc ggtgaccgcg cgctcgaaaa ggccggccgg
      481 aacgctcccc gcccaggtat cgaagatctg cagcgcctcg gcgcccgcct ccacctgcgc
      541 catcagatag gccgaggtcg cctcgaccag gcgatcgatc agcgccgacg cctcgctgac
      601 gctgaacttg ccccgcgctt ggcgttgggt caggccgtac tggtggcggg cggtggcaaa
      661 ggtgtacccg gctgcgtcga gcagcgcctg caggtaagcg atctgcttgg ccgacgcggg
      721 cgggccggat tgctgaccga aggccacggc gagctcagac cacgaagttg atcagccggt
      781 tgggcacgac gaacacgttc ttgaccgtcg ccccttcgag ggcggccgcg accggcgctt
      841 ccgcgcgggc gatgcgttcg atctcggccg cgtcgagatc gacggcgacg gtgagccgcg
      901 cccggatctt gccgttgacc tgggccacga tggtggtctc cgacttgacc agcagggccg
      961 gatcggcaac gggccagcgc tgctgatgca ccgacccctc ccgcccgatg cgctgccata
     1021 gctcgtcggt gatgaacggg cacaccgggg cgagcatggt caacaaggcg tcgatcgcct
     1081 cgcgcatcgg cgtgccgtgc actccagcgg ccgcggcctc gcgggccgcg ttggcaacct
     1141 ccatcagctt ggcgatcgcg gtgttgtact tcttgcgggt gaaatcgtcg ctgatcgcgg
     1201 cgatgcgagc atgcaccgcg cggcgcagcg tgtcgatcgc ttcgggcgaa ccggcgtcgg
     1261 ccacgctggc cgggccttcg gtcagcgcct ggcgccacac ccggttgagc cacttgttca
     1321 tgcccgacgg cgacacgtcg gcccagtcga tgtcgtcttc gggcgcggac gcgaacagga
     1381 tcgtggcccg cgccgtgtcg gcgccgtagt tgccgacgac ctcgcccggc gtcaccaggt
     1441 tgccgcgcga cttcgacatc gccgccccgt cgaggatgac ctggccctgg ttcagcaggg
     1501 cgttgaacgg ctcgctggcc tgcacgtagc cgaggtcgcg catcgctttg acagcgaagc
     1561 gggcgtacag caggtgcagg atcgcgtgct cgaccccgcc ggtgtactgg tcgaccggca
     1621 gcagcttcga ggccagatcg accggccacg cctgcgtgga gtcgtcgggt gacaggtagc
     1681 ggaagaagta ccacgacgag tcgacgaagg tgtccatcgt gtcggtgtcg cgcacggctg
     1741 gcgcgccaca agacggacag gcgacgtgct tccaggtggg gtgcagcgcc agcggcgacc
     1801 cgttgacgct ccagtcgaga tcggccggca accgcaccgg caggtcggcc tcgggcaccc
     1861 cctgggggcc gcacgcctcg cagtggatga tcgggatcgg cgggccccac gaccgctggc
     1921 gcgagatgag ccagtcgcgg aggcggaagt tgaccattcg cgccccccag cccttggcct
     1981 cgaggtcgtc catgatcgcg ttcttggcgt ccgcggtggt cagcccgtcg tagtcgccgc
     2041 tgttgacgac gatgccgtag tcggtgaaag cctgggtcat cgtcgccggg tcggccgcgg
     2101 ccagcgccgc ggggtcggcc gagtcgggcc gcaggatggc ccggatctcc aacccgttgg
     2161 ccttggcgaa ctcgaagtcg cgctggtcgc cgaagggcac ggccatgatc gcgccggtgc
     2221 cgtaatccat cagtacgtag tcggccgcgt aggcggggat gtgctcgccg gtgaacgggt
     2281 tgaccatgtc gaagttgagg cgcaggccgc gcttgacgac ctggcccggc ccgagcgtgg
     2341 cctggcgctc gatctcggac cgcttcgaga cctcggtcaa gaactcggcg taggccggat
     2401 cgtcggccat gcgcttggcg accaccgggt gctcgggagc gaacacgaag tacgtcgcgc
     2461 cgaagatcgt gtcggggcgg gtggtgtaca ccgcgacgtc gtcgccaccg tcggccgtgg
     2521 cgaagctgag ctgcgcccct tcggagcggc cgatccagtt gcgctgcatg gtcttgaccc
     2581 gctccggcca gtcgatcgtg gccaggtcgt cgagcaactc ctgcgcgtag cgggtggtgc
     2641 ggaagtacca ctggatcagt gggcggcgca cgaccacgtc gtcgctgcgc tcgcacaacc
     2701 cgttgacgac ctgctcgttg gccagcacgg tctggcaccc agggcaccag ttgaccatgc
     2761 cgtccttgcg gtaggccagg cccgcgtcga acagcttgag gaacagccac tgcgtccagt
     2821 ggtagtactc ggggtcgcag gtgtgcagac gggtggacca gtcgaagtcg tagcccaggc
     2881 ggcggatcga ggcggcctgc tgctcgatgt tggcgtaggt ccactcggcc ggatcgacgc
     2941 cgcgcttttt ggccgcgttc tcggcggggn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnngcc ggaggggcac cggcgcgatc ggccacccac cgctccgcga
     3181 gggggggttc gatcacgttc ggctcgtacg gtggaatgtc cgtctgctct ccaggttgcg
     3241 cgtgctcggc catagggcca tcaggctacg ggctggcatg ggcgacctga tgagggataa
     3301 cggcccggtc gggaatggac ggtctggtcg cgagaacgga cgatcgttgg acgcctcgat
     3361 ggtcagatcc atcgcatgtc attggcgacg actcttcctt cgaccgcatc accccgctcg
     3421 gcccgaccga cagcgccgcg acgaccacgc tcggtgctgt tgatgctgct cgcgctggtg
     3481 ggcgtgttgg tctccagcgt ggcggtcacc aacgcgatga tcgccaacgc cgcgccgacc
     3541 tcacccccac caggtccggt ggtcgcgccc ggtgcgccga gccgcgcgtg cgtgaacggc
     3601 gaagagggtc tgtccagcag ctacgcctac ggctactact ggtcggaggt cggcaggcgc
     3661 tgggtgcaac agccgatgtg cgcggtgctg tggagcggca tcacgttgat ggcgtcgcag
     3721 aagggcgcgc ccggcgacac cttcacgttc aacgtcacct cgctgaccaa cacgacggtc
     3781 tacggaccac cccgcaacgc catgtcgtgg tcgcatcccg gcacggtcgt cgcgggctgc
     3841 accggtgccg acttctcctg cacgttcaag ctcggcaccg tggggcagaa cctggccgag
     3901 tacgccacat cgctggtgtc ggtgtcgctg ccctactttt accgtgacga ctacccccag
     3961 atcggctgca cacccgagac gccatgtggg gcgtacacga ccgtcgccta tagctgggtg
     4021 acgttgaccc ccaactgggg tcaaccgacg gccgacttcg acgtcgacgt caccggcgac
     4081 cggatcgacg tcgtggccat ctcggccgac ccggtggcca agcgggagat gacccacgtc
     4141 tggacgatgg acagcgaggg cagcgccgag ggtgtcaccg cgacgcaccg cttcgcacgc
     4201 tcgggcgtgg tcgacgtgac gctggtgtcg accaccgacg acgggcgcac cgcgacgatc
     4261 accaagcagg tcgagatcga gcagtcgccg gtgacgatgg agctcgaccc cgacttcacc
     4321 caactcgcac cgggcaagac ctcgacggtc gacctggtgc tgacctcgac cgaccccgac
     4381 gcgtcgacca cggtcacgct cgacccggcc ttggtcgcca acaacgggct gttcacgatc
     4441 acgccaccgg cctcggcggc cagcccgatc accgtgccac ccaacggcgt ggtgcggttg
     4501 agcttcggcg tcgaagcggg cgacgacctc ggctccgaca cgttccgctc cgcgggcgtg
     4561 gtcgaatcgg ccagcggcaa gcagtggaac atccaggcga ccgcaccgac gctggtggcc
     4621 gagcacttcg tcaacgcggt gctcaccttc gacccggacg aggtcacgtt ggaagaggac
     4681 ggcgacggcc cggcaccgcg cacgatcaac ctccacctcg acatcaccaa cgacaccgac
     4741 cagacgctga ccgacctggt gctcgcgctg ggcaaccccg gctggcagtt ccccgcgtcg
     4801 ttcatcaccg cgacgccggg cgacgtggtg catctggccg acaccgagaa caccgaccgc
     4861 gagttcaccc tcggcgacct ggcgccgcac gcgtcgacca cgctcgacat ccccgtggtg
     4921 gtcaccgacg acggccgcat cgagtggggc ggcctgatcc gcctgacacc cgaagacggt
     4981 cgggagcgca ccgaagcggc caccaagggc aagctgacct ccgagccgaa gtacctggcc
     5041 cggttcgagt tcattgactg gcccagcggt caccgcgtca acgccgggct cgaccagaac
     5101 gtggccgaac cgtggaacac cgaccagatc gtcaaggctg gtcaggcctt cggcgtgctc
     5161 ggcaaggtga agaacctcac caccgatcag atggtgatgg ccgacctgcc gacgacggcg
     5221 ctggatcaca tcacctcggc cgtgttccgc ggcagcgccg acgagggcgc ggcgccgacg
     5281 accgacgagt tgctcaccgc cgggctcccc tatcgccaga cactggagcc gggtcagacc
     5341 gatgtggtca gcgcctaccc gtcaacggtg gaggacgagt cgttcggacc tggcgagacg
     5401 ttcagctcgc gcttcaccgt cgctcccgcg gcgtcggccc gcaacgagga tggctcgtac
     5461 cccgacgcgg tgcctgccaa ggtgctggcc gacgacgagg gcctgaccgc ctcggcggcg
     5521 acgatgaccc caccggccag cggcagcccg aacgccaacg tcggctacct cgggcggttc
     5581 tcgatcggct atgtcgaagg cggcatccgc ttcgtgaagg gctcggccgc gtcgatcgcg
     5641 tggctgctga ccaaggcgcc gcagcttccg ggcatgtcgg tggcggccgg tacccagttc
     5701 ctcaaggtcg ccagccgact gaccggcacg atggtcgcgg tcggcatcga gctggccgag
     5761 atgacacccg cccagcgcag cgcgctggtc gaccaggtga ccgcggaggc cgtcaacttc
     5821 ctggtgatga aagcgcccga cacgtcggcg gcggtgttcg aagcggtgca caacgcggtc
     5881 gatcaggcga tcgtgaagtt gatgacgttg gccatggacg accccggcca gttctgggag
     5941 agcatgggct cgttcaccgc cagcgcggcg ggcgagatca gcatcggcgt gctgaccgac
     6001 tcggtggtcg ccaaggtgat gacaaagctg cgcgtcggcg agggcctggc caaggccgag
     6061 cggttcctgg ccgaccaagc cgcggcggtg ggcctcaagc tggagaaggg tctcgccggc
     6121 atccgtgcgg gaacgatcct gaccgacgaa ctgctggcca aggcgatggg catcacccgt
     6181 aaccagatcg accagatgac cgtgatcgcc aagaacttcg gcgtcaacat ctacgcccgc
     6241 tcgcggggca gccgggcgat cgaactgatc gagaacctga ccgcgacgat gaagccgtac
     6301 ttcatcaagc cgaagagcgt caacgacatc gaccagttgt tcctcggctt cgaacggctg
     6361 cagcccgacg tggttgcgct gcgcaagccg ccgacgtggg ccgagctgtc cgagaagatg
     6421 gccgcctacc gcaccgaagc cggtgaggcg ctgaccgctg gcgagcagga gttggtgatc
     6481 agccgctgga agacccgcaa caaggagtgg tggggccaga gcggcgacat cggcatcgac
     6541 atcgacccca ccgatcacgc ctcgtacctg aagagcgagc gctacaagct ggagtcgaag
     6601 aacggcaagc tggtcgccct gacgctggag accgagggca acctggagga ccagatcgtc
     6661 aaggcgggcg acttcgccac cagcaagagc acgttccaac tggccgtcag ccccgacgac
     6721 gccgacgcgc tgatcccgat gctgcgcgcc cagggcgaga gcaacttcaa ggtcgtcgtc
     6781 ggtgacgtcg acctggtggc aatcacctac ccggacggca ccgtgccgcc gttggacgac
     6841 ctgatcaaga tctaccgcga gctccagaag gagccgctga acatgcagca ctcggtgacg
     6901 ctgacgtgga ccaaccacag cccggcgatc aagatgctcg gtgatcacgt cggccccaac
     6961 gccgagccgc tgctggagct cgccgtcagc ggcacccgcc gggcggtcaa gttcgacccg
     7021 gcgatgagct tcgccgacgg cgcctattac aagaagggcg cccagttcct acatatggcc
     7081 ggtggcgaga tcaacccgcc cggtgcgtac gcgaccgccg cggcgctggt gtggcaggtg
     7141 accgagccgg tcgtcaacct gtactcgccg cccggtgcgt acggggccga catgaagcgc
     7201 tcggccccac cgacccgcgt cggcgaggac ggcaccgaag tgttggtcga cggcgtgtgg
     7261 caacccggcg gcgcttcggc gcagcaggcg catcaggccg aggcgatccg ctacgcggag
     7321 accgtgtcat cggccgcggc cgagatgccc gagccgccgc tgccgagcgt ggtcggcgta
     7381 cagagcgcga tcgatctgga ggtcgccgcc ggggcgacga cgctgccggt gctgggccgc
     7441 gccgcgctgt atcccttcgg cggggccgcc nnnnnnnnnn nnnnggcgac acggtgatcg
     7501 tcgaccccgg caccccgggt gcgttccaga ccacgttgac cgcggtgagc gagacgtcgc
     7561 tgactttggc caccgcggtc cccaccgcaa tcgtgcgcgg cacgatgatc gtgatgctgc
     7621 ctgccgcgga cgaaccgccc acgaccacca gcgatccgac cagccccacg tccagcgagc
     7681 tggccaccac gaccaccaac gacgcaggca gcgccaccac ggcgacaccg accgcgccga
     7741 cggcaccgag cggcggcacg
//
LOCUS       DAORNF010000043         7718 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_7000_length_7718_cov_1.937753, whole genome shotgun sequence.
ACCESSION   DAORNF010000043 DAORNF010000000
VERSION     DAORNF010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 7718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7718
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7000_length_7718_cov_1.937753"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="PLV68_02845"
     CDS             complement(<1..342)
                     /locus_tag="PLV68_02845"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HRE00208.1"
                     /translation="MSDDQLPIQTSVMMPGGDAVAAALEALGVTVVFGIVSIHNVPIL
                     DAIARSATIDFVACRHEQGAVHAADGYARATGKLGVALTSTGPGAANAMGGLFEANYA
                     STPVLMITGQTE"
     gene            complement(332..1576)
                     /locus_tag="PLV68_02850"
     CDS             complement(332..1576)
                     /locus_tag="PLV68_02850"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRE00209.1"
                     /translation="MTPSVETDDEFRARVRDFLAVNADVEGWRRDRFGRAPDDGATNE
                     VRLRRNRRCMTLLHEAGLGALSWPQRYGGQGLTKRHQVIFNQEVAPFSLPLTMFIIGQ
                     GMCGPTLLAYGTEEQRDRYLPPLLRGEEIWCQLFSEPGAGSDLASLRCRADRDGDSWV
                     INGQKVWTSGAHNARFGLLLARSDAASMRNAGLTAFVVDMDNPGVDVRGLVEMTGVTR
                     FNEVFFTDCRLAAGSVIGTVGEGWRCAITTLMNERVSIGTSSPGGYGFPSSVLIDEAR
                     RTRRLAEPVLRDEIARIVVEERLVEFLGMRVTEALLAGVEPGPEGSIAKLAGTRLSKR
                     SAALAMEIVGPQAIAWDPAFPGGRTWADVTSAAPGLSIAGGTDEILRNILAERVLGLP
                     RDARPETATASQLERTGADHER"
     gene            complement(1620..2408)
                     /locus_tag="PLV68_02855"
     CDS             complement(1620..2408)
                     /locus_tag="PLV68_02855"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRE00210.1"
                     /translation="MTDSSLPTNSTRDLAGRVVIVTGAARGLGRDYARLLAADGAKVA
                     IADVRADAVAATADELVRSVPSADVVAITVDVTDPASTSALAAAVVARWGTIDVLINN
                     AGIWGDLEPAPLWATDPAYFDRVLAVNLRGPLLCAQAVVGAMREQGWGRIINISSMGA
                     YMPSGVYGVSKLGLNQMTFTLATELGPDGITVNAVAPGPIDNEATRSQVAEAGITKMI
                     AGTALKRMGTAADLYGMIRYLASTDADWVTGQTFLVNGGYNSRL"
     gene            complement(2463..>3442)
                     /locus_tag="PLV68_02860"
     CDS             complement(2463..>3442)
                     /locus_tag="PLV68_02860"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRE00211.1"
                     /translation="RRNWLALAWPVDAGGEGKHPLYEFVLWNEMARCRAARPPLGSGI
                     VAKTIIAAGTDEQRQRFLGPLRLAEIAFSLGYSEPEAGSDLAGLRTTAVRDGDHYVLT
                     GEKRWTSGAHRADYLWVLCRTGPQDQRGRALSLLIVDRRSPGITISPIPAVGGERFNE
                     VFFDAVEVPVANRVGPENGAWQLMGSSLATERHVQFSPARVAQDFDDLLAWLREQGLI
                     DDPIVRHRVGELAVQVAEVEAHALAMLECVQDGRDAAVEAAANKLAGSEAAQAIARAA
                     GDLGAPESVVVGSPIEFGWRQSISETIGGGTSEIMRGVIAGQGLGLPLR"
     assembly_gap    3443..3452
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3702..3812
                     /estimated_length=111
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3813..>4340)
                     /locus_tag="PLV68_02865"
     CDS             complement(<3813..>4340)
                     /locus_tag="PLV68_02865"
                     /inference="COORDINATES: protein motif:HMM:NF027439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HRE00212.1"
                     /translation="PDRGNALHASQVEPIKAIWAEVRTDPWIRCAIVTASGDRHFCTG
                     ADVGAVASRGGVRAGRGPLSDEVFWSPRQNRVWKPVICAVNGTVASAGLHFVVDADIV
                     VASTNAQFLDTHVNVGMVGAVENIGLAKRLPLGSALRMTLCGKAYRMSADRAYQLGLV
                     DELAEVGSRFTHHSSI"
     assembly_gap    4341..4369
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4489..>4900)
                     /locus_tag="PLV68_02870"
     CDS             complement(4489..>4900)
                     /locus_tag="PLV68_02870"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRE00213.1"
                     /translation="AIAAYLVGAASELLRLATEYARDRVQFNRPIATFQAVSHPLATA
                     STRLNASRILARRAAQHVDADRDDAPASAATARLSAVGSASAIASQAHQLFGAMGFTL
                     EGPVAHLSHRIRQVGLLAPDQRRSRALVVDHALA"
     assembly_gap    4901..4933
                     /estimated_length=33
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4934..5215)
                     /locus_tag="PLV68_02875"
     CDS             complement(<4934..5215)
                     /locus_tag="PLV68_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00214.1"
                     /translation="METLGRHGAPGPLVGTYLVTALVDDEVAAPIADGRTLVSVGQGT
                     LFPWAPVAGVFVSIDGDQAWRVQPAGAIEPLTTTVGEPWGRFEPVAVESL"
     gene            complement(5230..5418)
                     /locus_tag="PLV68_02880"
     CDS             complement(5230..5418)
                     /locus_tag="PLV68_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00215.1"
                     /translation="MSLRLSYDDHQQALQESIDGFCRRSGTGPLFAHDNPLPPEFWHG
                     LADLGVLALGTDAGGAAR"
     gene            5493..6476
                     /locus_tag="PLV68_02885"
     CDS             5493..6476
                     /locus_tag="PLV68_02885"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HRE00216.1"
                     /translation="MTSRAGRWPDASPVGRGHVLLATTAFADEYADRLLAAAPGLGVV
                     RLDESAAPSALDTVTIAFMSKDAWPAQARPLLERAAAAPRLEWLHVMSAGIDGGAFDH
                     FRDRGVRVTRSAGASANAIGESVFMFLHALTRTAHRGRSSPHHIEWHRWRELAGRTIA
                     VLGYGPIGQRIVELALAYGMQPTIVRQHARGDEPCPTVSISALEDVAGHHDVVVVALP
                     LNEQTRGVVSRSVIERMGPDAVLINVARGALVDQRALTDALRQRRIGGAGLDVFEVEP
                     LPPDDPLWDLPNVVITPHNAGSSEGGPRLVAEIFFANLERFLTGAPLLHEV"
     gene            6526..>6934
                     /locus_tag="PLV68_02890"
     CDS             6526..>6934
                     /locus_tag="PLV68_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00217.1"
                     /translation="MSLHNDGLAGGPPKFYAILATPGGTLVAGSCVRGLSRSIDGGIG
                     WEPVDGLDHVSINTLVCAPDSSILAATSGGLWRSIDEARSWQQIDDRPRYAVLPGGVQ
                     PLPDQVVFRLLALRDGRTIAGTDGAGVWVTGGSS"
     assembly_gap    6935..6947
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6948..>7324
                     /locus_tag="PLV68_02895"
     CDS             <6948..>7324
                     /locus_tag="PLV68_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00218.1"
                     /translation="LEDTTIYSLAETAAGTLLAGCRGRGLARSDDGGITWTTSNEGMD
                     DVSVHCLLQRQAGDILAGTGQGIRRSTDDARSWMPYADELHGHRIFALTTVDDVNVAA
                     GSYTNVWIGTGERWRQVDPGLTP"
     assembly_gap    7325..7340
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7341..>7718
                     /locus_tag="PLV68_02900"
     CDS             <7341..>7718
                     /locus_tag="PLV68_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00219.1"
                     /translation="SVTVDHVGDLYAGTKSGIRRSTDSGATWHTETTRPVTFRVATTA
                     AGQVLAAGDAGVQLSPHWEPLGVLGRRAWAVFEVGPGHVLAATLTEGIFQYLDGTWSP
                     LPNGPPDWQAYCFLRSSTGRILV"
BASE COUNT         1235 a         2560 c         2499 g         1212 t
ORIGIN      
        1 ctcggtctgc ccggtgatca tcagcaccgg tgtcgaggcg tagttcgctt cgaacaggcc
       61 gcccatcgcg ttggcagctc cgggaccggt gctcgtcagc gcgacgccga gcttgccggt
      121 ggcgcgtgcg tagccgtccg cggcatgcac ggcgccctgt tcgtgacgac aggcgacgaa
      181 gtcgatcgtg gccgacctcg cgatggcgtc gaggatgggc acgttgtgaa tgctgacgat
      241 gccgaagacg acggtgacgc cgagtgcttc gagcgctgcg gccacggcgt caccaccggg
      301 catcatcaca cttgtctgga tgggcagttg gtcatcgctc atggtcggct ccggttcgtt
      361 cgagttgaga cgcggtcgcg gtctccggtc gggcatcgcg cggcaagccg aggacacgtt
      421 cggcaaggat gttgcgcaag atctcgtcgg ttccgcccgc gatcgacagg cctggcgcgg
      481 ctgacgtgac atcggcccag gtccgtccgc cggggaatgc cgggtcccac gcgattgctt
      541 gcgggccgac gatctccatc gccagtgccg ccgatcgctt cgacagtcgc gtcccggcga
      601 gcttggcgat cgatccctcc gggccgggtt cgacaccggc caggagtgcc tctgtgacac
      661 gcatgcccag aaactcgacg agccgctctt cgaccacgat gcgcgcgatc tcgtcgcgca
      721 gcaccggttc ggcgagccgg cgcgtacgcc gcgcttcgtc gatcaggacc gacgatggga
      781 atccgtaccc gccaggcgaa ctggttccga tcgagacccg ctcgttcatc agggtggtga
      841 tcgcgcaacg ccaaccctcg ccgacggtgc cgatgaccga cccggcggca agccgacagt
      901 cggtgaagaa cacctcgttg aagcgcgtca cgccggtcat ttcgaccagt ccgcgcacgt
      961 cgaccccggg gttgtccatg tcgacgacga aggctgtgag accggcattg cgcatcgacg
     1021 ctgcatcgga gcgggcaagc aacagtccga accgcgcgtt gtgggcaccc gaggtccaga
     1081 ccttctgacc gttgatcacc caagagtcgc catcgcggtc ggctcggcat cgtagggacg
     1141 cgaggtcgga tccggccccc ggctcggaga acaactgaca ccaaatctcc tcgccacgca
     1201 gcagcggtgg gaggtagcgg tcgcgctgtt cctcggtgcc gtaggccagc agcgtcggac
     1261 cgcacatccc ctgaccgatg atgaacatcg tcaacggtag cgaaaagggc gcgacctcct
     1321 gattgaagat cacctgatgc cgcttcgtga gcccctggcc gccgtatcgc tgcggccatg
     1381 agagggcacc caagccagcc tcgtggagca acgtcatgca ccgccggttg cggcgaagcc
     1441 gcacctcgtt ggtggcacca tcgtcaggcg cacgaccgaa gcggtcgcgt cgccaaccct
     1501 ccacatcggc gttgaccgcg aggaagtcac ggacacgtgc ccggaactcg tcgtcggtct
     1561 caacggacgg agtcacgttg gaagccatcg gtccgaacgt cgacacagac gcgaccacgt
     1621 cacaaccgac tgttgtagcc gccgttgacc aggaacgtct gcccagtcac ccagtcggca
     1681 tcggtgctgg ccagataacg gatcatcccg tacagatcag ccgcggttcc cattcgcttc
     1741 aacgccgtcc cggcgatcat cttggtgatg cccgcctccg cgacctgcga acgcgtcgcc
     1801 tcattgtcga tcgggcccgg cgcgacggcg ttgacggtga tcccgtccgg gcccaactcg
     1861 gtggcgagcg tgaaggtcat ctggttcagg ccgagcttcg agacgccgta cacgcctgac
     1921 ggcatgtagg cacccatcga ggagatgttg atgatgcgac cccacccctg ctcgcgcatc
     1981 gccccgacga cggcctgcgc gcacagcaac ggtccgcgaa ggttcacggc caggacacga
     2041 tcgaagtagg cggggtccgt cgcccacagc ggcgccggct cgagatctcc ccagatgccg
     2101 gcgttgttga tcagcacgtc gatcgttccc catctggcga cgaccgcggc ggccaacgcc
     2161 gacgtgctcg cgggatcggt cacatcgacg gtgatcgcga cgacgtcagc gctgggcaca
     2221 gagcgaacca gttcgtcggc ggtcgcggcg acagcgtctg cgcgcacatc tgcgatggcg
     2281 accttggccc cgtccgcggc gaggagccgg gcgtagtcgc gtccgaggcc acgggcagcg
     2341 ccggtgacga tgacgacacg accggccagg tcgcgggtcg agttcgtggg aaggctggag
     2401 tcggtcattg cgctctcgat cagtcgttgg tgatggatct actggaaggg tggtggacgg
     2461 ggtcaacgaa gcgggagccc gagtccttga ccggcgatga caccgcgcat gatctccgag
     2521 gtgccaccgc cgatggtctc gctgatcgac tgccgccacc cgaactcgat cggcgagccg
     2581 acaacgaccg actcgggcgc gccgaggtcg ccggccgccc gcgcgatcgc ctgggcggct
     2641 tcgctgccag caagcttgtt ggctgccgcc tcgaccgcgg catcacggcc gtcctggacg
     2701 cattccagca tcgccaacgc atgagcttcg acttcggcga cttggacggc aagctcgccg
     2761 acacgatgac gaacgatcgg atcgtcgatc agtccttgct cgcgcaacca cgccaggaga
     2821 tcgtcgaagt cctgcgcgac ccgagccggt gagaactgca cgtggcgctc ggttgcaagt
     2881 gacgagccca tgagctgcca ggcgccgttt tccggcccga cgcgattcgc gaccggcact
     2941 tcgaccgcgt cgaagaacac ctcgttgaac cgctccccac caacggccgg gatcggcgag
     3001 atcgtgatcc cgggtgagcg ccgatcgacg atcagcaggc tcagcgcccg tccccgttgg
     3061 tcttgcgggc cggttcggca gagcacccac aggtagtcgg cgcgatgcgc ccccgaggtc
     3121 cagcgcttct caccggtgag cacgtagtga tcgccgtcgc ggaccgccgt ggtccgcaat
     3181 ccggccaggt cggacccggc ctctggctcg gaatacccga gcgagaaggc gatctcggcc
     3241 aggcgcagcg ggccgaggaa gcgctgcctc tgttcgtccg tgccggctgc gatgatggtc
     3301 ttggcgacga tgccgctacc cagcggtggt ctcgccgcgc gacaacgcgc catctcgttc
     3361 cacaacacga actcgtagag gggatgtttg ccctcgccac cagcgtcgac cggccacgcc
     3421 agggcaagcc agttacgacg gcnnnnnnnn nngatacagc tcgacgattg ccacgtggcg
     3481 atcaccctcg tgatgaaaga agccgttcgt atcgaggtag ccacggagaa acgcccggac
     3541 ctcgtcgcgg aactcgatca gttcggggcc aagctcgaag ttcatccgtg cgcgtcgtcc
     3601 acgttctcgc cggtctccca cacaggtgcc cgccgctcga caaacgcctt ggggccctcc
     3661 ttgaagtcgg gatggtccca gtgcatgcgc aacagcgccc annnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngatggagc tgtggtgggt gaagcggctg
     3841 ccgacttcgg ccagctcgtc caccagaccg agttgatagg cgcggtcggc cgacatgcgg
     3901 tacgccttgc cgcacagcgt cattctcagc gcagatccga gcggcagccg cttcgcgagg
     3961 ccgatgttct cgaccgcacc caccatgccg acgttgacgt gcgtgtcgag gaactgggcg
     4021 ttggtcgacg cgacgacgat gtcggcatcg acgacgaagt gcaaacccgc cgaggcaacc
     4081 gtcccgttca ccgcgcagat cacgggcttc cacaccctgt tctggcgcgg cgaccagaag
     4141 acctcgtcgg acaacggccc gcgaccggcc ctgaccccac cgcgggatgc gacggcaccc
     4201 acgtcggcac cggtgcagaa gtggcgatca cccgacgcgg tgacgatcgc gcatcggatc
     4261 cacggatcgg tgcgcacctc ggcccagatc gccttgatcg gttcgacctg gctcgcgtgc
     4321 agggcattac cccggtcggg nnnnnnnnnn nnnnnnnnnn nnnnnnnnna tgtaggcgac
     4381 gtggtccttg cgttcgtatc gcaagccctc gagcggggcg gtgagatcta ccatcgtgat
     4441 gtcctttcga gagtttccgg gtcgggtctg gtctgggtgg gggttcggtc acgcgagggc
     4501 gtggtcgacg acgagtgcgc gcgatcgtcg ctggtcgggc gcgagcagcc cgacttgacg
     4561 gattcgatgg ctgaggtggg cgacgggacc ttcgagcgtg aaccccatcg caccgaacag
     4621 ctggtgtgct tgagacgcga tcgccgacgc tgatcccacc gccgacagcc gcgccgtcgc
     4681 cgcggacgcg ggcgcgtcgt cacggtcggc gtcgacatgc tgcgcggcgc gtcgagcgag
     4741 gatgcgtgag gcgttgagcc gcgtggatgc agtggcgagg gggtgcgaga ccgcttgaaa
     4801 ggtggcgatc gggcggttga actgcacccg atcgcgcgcg tactccgtcg ccaggcgcaa
     4861 cagctcggac gcggcaccga cgagataggc cgcgatcgcc nnnnnnnnnn nnnnnnnnnn
     4921 nnnnnnnnnn nnngagcgac tccaccgcga ccggctcgaa tcggccccat ggctcgccga
     4981 ccgtggtcgt gagcggttcg atggccccgg caggttgcac cctccatgct tgatcaccgt
     5041 cgatcgacac gaatacaccc gcgaccggag cccatggaaa caaggtgccc tgcccgacgc
     5101 tgacgagcgt tcgcccgtcg gcgatcggag cagcgacctc gtcgtcgaca agggcagtga
     5161 ccaggtaggt gcccacgaga ggcccggggg ccccgtggcg acccagcgtc tccatcgctg
     5221 cggcgatctc taccgcgccg ccccgcccgc atcggtaccg agcgcgagca cgccgaggtc
     5281 ggccagtcca tgccagaact ccggtgggag cgggttgtcg tgcgcgaaca atgggccggt
     5341 acccgatcgg cggcagaacc cgtcgatcga ttcctgcagc gcctgctggt ggtcgtcgta
     5401 ggagagtcgc aggctcacgc caacatcacc cgttcgccgc gcaccgtcag atcctccccc
     5461 atactcatca cctttcagta tactcattcg ctgtgacgag tcgggctggt cggtggccag
     5521 acgcatctcc ggtcggacgc ggccatgtac tgctggcaac caccgcgttt gccgacgagt
     5581 acgccgaccg gttgctcgcc gcggcgccag ggctcggcgt ggttcggttg gacgagtcgg
     5641 ctgctccttc ggccctcgac accgtgacca tcgcgttcat gtccaaggac gcatggccgg
     5701 cgcaagcacg gccgttgctc gaacgcgccg cggcggcccc ccggctcgaa tggttgcacg
     5761 tcatgtcggc gggcatcgac ggtggcgcct tcgaccactt ccgcgatcgg ggtgtgcggg
     5821 tcacccgttc ggcgggcgcg tcggcgaacg cgatcggcga gtccgtgttc atgttcctcc
     5881 acgcactgac ccgcaccgct catcggggcc gatcctcccc tcaccacatc gagtggcacc
     5941 gctggcggga gctcgccgga cgcacgatcg cggtcctcgg ctacggtccg atcggccagc
     6001 gcatcgtcga gctcgccctg gcctacggca tgcaaccgac catcgtgcgt caacacgccc
     6061 gcggcgacga accgtgtccg acggtctcga tctccgcact cgaggatgtg gcgggccacc
     6121 atgacgtcgt cgtggtggcc ctccctctca acgaacagac acgcggcgtc gtgtcccgca
     6181 gcgtcatcga gcggatggga ccggatgcag tgctgatcaa cgtcgctcgc ggcgccctgg
     6241 tcgaccaacg cgcgctcacc gatgcgcttc ggcaacgacg catcggcggc gcagggctgg
     6301 acgtcttcga ggtggagccg ctaccgccgg acgatccgtt gtgggacctg cccaacgtgg
     6361 tgatcacacc acacaacgcc ggctcgagcg agggcggacc acgcctcgtg gcagagatct
     6421 tcttcgccaa cctcgagcgg ttcctcacgg gcgcgccact gctccacgaa gtgtgatccg
     6481 atccccactg ccagccgggt cacgcgagat cagtatactg atcttgtgtc actccacaac
     6541 gatggactgg cggggggacc gccgaagttc tacgccatcc tggcgacgcc gggcggcacc
     6601 ctcgtcgctg gttcctgtgt gcggggtctg tcacggtcca tcgacggggg catcggctgg
     6661 gagcccgtcg acggactcga ccacgtcagc atcaacacgc tggtgtgcgc acccgactcg
     6721 tcgatcctcg cggcgacgtc ggggggtctg tggcggtcga tcgacgaggc ccgatcgtgg
     6781 caacagatcg acgaccgccc gcgctacgcg gtgctgccgg gtggcgtcca accgctgcca
     6841 gaccaggtcg tgttccggct gctcgcgctc cgcgacgggc ggaccatcgc gggtaccgac
     6901 ggcgcaggcg tctgggtgac cggtggttct tcggnnnnnn nnnnnnngac tcgaggacac
     6961 cacgatctac tcgttggcag agaccgcggc cggcacgctg ctcgcgggct gccggggacg
     7021 tggcctggcc cgctctgacg acggtggtat cacgtggacg acctcgaacg agggtatgga
     7081 cgacgtctcc gtgcactgcc tgctgcaacg ccaagcgggc gacatcctcg ccgggaccgg
     7141 tcagggcatc cgccgatcga ccgacgacgc ccgatcgtgg atgccgtacg ccgacgagtt
     7201 gcacgggcac cgcatcttcg cgttgacgac ggtcgacgac gtcaacgtcg ctgctggcag
     7261 ctacaccaac gtctggatcg gtaccggcga gcgatggcgt caagtcgacc ccggtctgac
     7321 tcccnnnnnn nnnnnnnnnn ggtcggtcac ggtcgaccac gtcggcgatc tctatgccgg
     7381 caccaagtcc ggcattcgcc gttcgaccga cagcggcgcc acctggcaca ccgagacgac
     7441 cagaccggtc acgttcagag tcgccaccac ggcggcagga caggtgctcg cagcgggtga
     7501 cgcaggtgtg caactcagcc cgcattggga gccgctgggt gtgctgggac gccgggcgtg
     7561 ggcggtgttc gaggttggcc ccggacacgt gctggcggca acgctgaccg aagggatctt
     7621 ccagtacctc gacggcacgt ggagcccgct cccgaacggc ccgcccgact ggcaggcgta
     7681 ttgctttctc cgttcctcga ccggtcgaat cctcgtcg
//
LOCUS       DAORNF010000044         7707 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_7012_length_7707_cov_1.742420, whole genome shotgun sequence.
ACCESSION   DAORNF010000044 DAORNF010000000
VERSION     DAORNF010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 7707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7707
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7012_length_7707_cov_1.742420"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1051)
                     /locus_tag="PLV68_02905"
     CDS             complement(<1..1051)
                     /locus_tag="PLV68_02905"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HRE00220.1"
                     /translation="MTSLELLDAYLARVDALNPALNAIVVDDRERARKAAKAADRAVQ
                     AGKKLGPLHGVPMTVKEAFAIPGHPVTYGSPALADNVLDIDAVAVARLRAAGAVIFGK
                     TNVPIGLSDFQSYNAVYGTTNNPWDTSRGAGGSSGGSAAALAAGLTALEFGSDIGGSI
                     RNPAAYNGVYGHKPTWGIIPERGHSIVAQPGAEGAISVVGPLARSAGDLALALRVTAG
                     PDLEFGYRLDLPAPPTSVKGLRIAVWLREPELGPVDEEVVAPIEAAAKALAQAGAKVS
                     FTARPAFDPRAAHDTYMQILMAAMSTGVPDFAAMVRRAERLDPDDRGPHAFNLRASTA
                     RYRDVALAQNAQARIR"
     gene            complement(1338..2156)
                     /locus_tag="PLV68_02910"
     CDS             complement(1338..2156)
                     /locus_tag="PLV68_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00221.1"
                     /translation="MTAALLASAGAGALIGGPTGAGAAADVAGVVRVDGTDTGTATTD
                     TSGAATSTPAADSTDGRPATPSRDGDRGPRGDGGMALEELVADGTITQEQADAVTAAV
                     KAAREAARTTDESATSTERPARGEVTSGVLAALVADGTITQAQADAVIAAREAARPDG
                     GFGGDGHGGRGGPGGGFGVGRGASAETAATAIGIGTDELQAELETGKSIADVATEHGV
                     AVQSVIDAIVAEQQTKLVERVTDMVNGVRPERPSRSQHASGDTAADDSSATTGD"
     gene            2409..>2529
                     /locus_tag="PLV68_02915"
     CDS             2409..>2529
                     /locus_tag="PLV68_02915"
                     /inference="COORDINATES: protein motif:HMM:NF014068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00222.1"
                     /translation="MVSSTPLQPIHDVVVIAYDGIQSLDVTGPYEVFAGANSVL"
     assembly_gap    2530..2562
                     /estimated_length=33
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2563..3524
                     /locus_tag="PLV68_02920"
     CDS             <2563..3524
                     /locus_tag="PLV68_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
                     /protein_id="HRE00223.1"
                     /translation="RPTIVSIDGGPVTTESGLQILTTPASDSPDTTRPVDTLLIPGGS
                     GSRTACTDPRLVATVTELAGRAQRVVTVCTGAFVAAGAGLADGRTVTTHWARAAELRR
                     RFPSVMVDADPIYIRDGDLWSSAGVTAGIDLALALVEHDHDAEVAQVVARWLVMFLRR
                     PGGQTQFAAPVWTERAVTTPVRAAQELVDAEPGGDHRVDALAARVGMSPRHFTRLFTT
                     ETGLSPARYVTEVRVEAARRALESTADAVEVIARRCGFGTAETMRRTFTRRLGLSPDQ
                     YRQRFRHQPPGHRTDHPNHHDHPNHHDHPNRHDHPNRHERSNP"
     gene            3521..4165
                     /locus_tag="PLV68_02925"
     CDS             3521..4165
                     /locus_tag="PLV68_02925"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HRE00224.1"
                     /translation="MTIHVAIPLFPRLTALDAIGPYEVLQRIPEIEVTFVGHERGEVR
                     TENGLLGLTRDATFEERPTPDVIVFPGGVGTRTVQHDERVLGWLRHAHATTTFTTSVC
                     TGSLVLGAAGLLDGLTATSHWATYPELEATGATPTAQRVVEHLDRRIITAAGVSSGID
                     MALRLVELLVDATAAEAAQLMIEYDPQPPFDSGSVAKSSQAVVERVIEYAQLRS"
     assembly_gap    4183..4197
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4228..4524)
                     /locus_tag="PLV68_02930"
     CDS             complement(4228..4524)
                     /locus_tag="PLV68_02930"
                     /inference="COORDINATES: protein motif:HMM:NF014505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HRE00225.1"
                     /translation="MTPSAGDVWCADRNDERRRLVYIVSDHRFHRLAGRVVIAPVLDA
                     SPGPQRPWYVPIAEGVVAANQFATVPVDRLLERVSRSDLPALQRVRRAVHNIVG"
     gene            complement(4521..4736)
                     /locus_tag="PLV68_02935"
     CDS             complement(4521..4736)
                     /locus_tag="PLV68_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00226.1"
                     /translation="MKISTATRDRLRVLGAEGDSLEDVVVHALDAFEAQQFWTAAEAA
                     LAVETAAERDHRLRVEAETDAWMGELG"
     gene            complement(4810..5433)
                     /locus_tag="PLV68_02940"
     CDS             complement(4810..5433)
                     /locus_tag="PLV68_02940"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HRE00227.1"
                     /translation="MPGWDHEPLRAVRAAHPDLVLQVDANAGYTPDVADLLVSLDRYG
                     LLMIEQPFAPEEWTAHAALVRRMATPICLDESILSVATTRLAIDLGACSIVNIKIGRV
                     GGVMESRRIHDACAERGVPVWCGGMLETGVGRAANVALAALANFAFPADISASERYWR
                     RDITEPFVLRGSTIAVPTGAGLGVTVDEAALTALGAARIPLPLAPLG"
     gene            complement(5414..>5852)
                     /locus_tag="PLV68_02945"
     CDS             complement(5414..>5852)
                     /locus_tag="PLV68_02945"
                     /inference="COORDINATES: protein motif:HMM:NF014766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00228.1"
                     /translation="VHVVTDVGEGWGECAAHAEPFYNEEFTDASVLVLQRWLVPAVAA
                     LNDTGDLTGVTAGAAMRHVKGYPAAKAGLEMAILDAELRASDVSLAQYLGATRTRIDV
                     GVSIGITPTVDELVAQVDGYVAYKQLTDPKPAGEAQDHAGVGP"
     assembly_gap    5853..5862
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5952..6110)
                     /locus_tag="PLV68_02950"
     CDS             complement(5952..6110)
                     /locus_tag="PLV68_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00229.1"
                     /translation="MNAHDDPRLAAQEAGVTVAPAASLDELGVLQATTDAVWVAEIAP
                     PRNMRHRS"
     gene            complement(6227..>7707)
                     /locus_tag="PLV68_02955"
     CDS             complement(6227..>7707)
                     /locus_tag="PLV68_02955"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRE00230.1"
                     /translation="APVRPDGTATLIDLMEGVEDIPGSALVDGMPWGSWESFADYLDV
                     LAGRRYAVDIAAQLAHGALRYYVMGDRGVANEDATADDIAEMARLTREAFAAGAVGFS
                     TSRTIGHRSKSGTPVPGTFAAVDELVGIAEAMGSVGHGVFEAIVASTIGRLDGLGGER
                     ASMSEEFELLRAVQAASGRPVTFTVAQLFEDPDGWRRMLDTSAAANAAVGSLAFRPQI
                     IPRSVTVMTSLDTYHMFMLRPTYQKLAHLGLAERAAEMHKPEVKAAILTETDDPAGGL
                     ARGLAMLFTNALPVTFPLTEPVNYEPSLDRSVLAQAMARGLDVAELMYDLLTANGGND
                     FYMVLGSNFVGGNLDVCKDMLLDPNTVTGLSDAGAHVNLISDCSSTTFHLTHWARDRV
                     DGLPVETVVAKMTANNADLYGFNDRGRLVEGLRADINVVDLDRLTIHAPFVRYDLPSG
                     ASRILQPSTGYVATLVNGVAVRRNDADTGERPGRLARGGPTA"
BASE COUNT         1199 a         2718 c         2562 g         1170 t
ORIGIN      
        1 agcggatgcg ggcttgggcg ttctgagcga gggcgacgtc gcggtagcgg gcggtcgacg
       61 cgcgcaggtt gaaggcgtgc gggccacggt cgtcggggtc gagccgttcg gcgcggcgca
      121 ccatcgccgc aaagtcggga acaccggtcg acatcgcggc catcaagatc tgcatgtacg
      181 tgtcgtgcgc ggcgcgcggg tcgaacgctg ggcgagcagt gaagctcacc ttcgcgcctg
      241 cctgcgccag cgccttggcc gcggcttcga tcggggcgac gacctcctcg tcgaccggac
      301 cgagctcagg ttcgcgcagc cacacggcaa tgcgcagtcc cttgaccgac gtgggtggcg
      361 ctggcaagtc caagcggtag ccgaactcca ggtcgggtcc agcggtgacg cgcaacgcca
      421 acgcgagatc acccgcgctg cgggcgagcg gaccgacgac cgagatcgcg ccctctgccc
      481 cgggctgggc gacgatcgag tgcccccgct cggggatgat gccccacgtc ggcttgtggc
      541 cgtaaacgcc gttgtaagcg gccggattgc ggatgctgcc accgatgtcg ctgccgaact
      601 ccagcgctgt gaggcccgcg gccaacgccg cggccgaccc gccggacgag ccgcctgcgc
      661 cgcgtgaggt atcccacggg ttgttggtcg tgccgtacac cgcgttgtac gactggaagt
      721 cggacagccc gatcggcacg ttggtcttgc cgaagatcac cgctcccgcg gcccgcagcc
      781 gtgccacggc aaccgcatcg atgtccaaca cgttgtcggc cagcgccgga ctgccgtagg
      841 tgacgggatg accggggatc gcgaacgcct ccttgaccgt catcggcacg ccgtgcaacg
      901 ggccgagctt cttgccggcc tgcaccgctc gatcggcagc cttcgcggcc ttgcgcgccc
      961 gctcgcggtc gtcgaccacg atcgcgttca acgctgggtt caacgcatcg acacgggcga
     1021 ggtaggcatc gagcaactcg agcgaagtca cccgacccga acgcaccgcg gccgccaact
     1081 gcgtggccga cccgaacgga aacgctcgca gtgcctccgc ccgagacggc agaggcgatg
     1141 tcgtgcgaga cgatctgcga gttgatgtag ccatgggact ccccctcgtt gctgcaaccg
     1201 gcaccagcga ccgtagccac ctcgcggttg acggtccgtg gtcgggacgc gacgaagccc
     1261 cagcatctgg ctgtcggatc gggccgccgc gggccagatt ggacagccag atgccagggc
     1321 tgagcgggag ggtacggtca gtcgcccgtg gtggcgctgc tgtcgtcggc cgcggtgtcg
     1381 ccgctggcgt gctggctgcg ggatgggcgc tcaggccgca caccgttgac catgtcggtg
     1441 acccgttcga cgagcttcgt ctgctgttcg gcgacgatgg catcgatcac gctctgcacg
     1501 gccacaccgt gttcggtggc cacgtcagcg atcgacttgc ctgtttccaa ctcggcctgc
     1561 aactcgtcgg tgccgatgcc gatcgccgtc gccgcggttt cggccgaggc tccccggccg
     1621 acaccgaacc ctccgccggg accgccccgg ccaccgtggc catcgccgcc gaagccaccg
     1681 tcaggccgtg cggcctcgcg ggcggcgatg accgcgtcgg cttgcgcctg ggtgatcgtg
     1741 ccgtctgcga ccaaggccgc gagtacaccg ctcgtcacct cgccgcgggc tggacgctcg
     1801 gtgctcgtgg cggactcgtc cgtggtcctg gcggcttcac gagccgcctt gacggcggcg
     1861 gtgaccgcat cggcctgctc ttgcgtgatc gttccatctg caacgagttc ctccagagcc
     1921 atcccgccat cgccgcgcgg cccgcggtcg ccatcacggc tcggagtagc aggacgacca
     1981 tccgtcgaat ccgctgcggg ggtgctcgtc gccgccccac tggtgtcggt ggtcgccgtt
     2041 cccgtgtcgg tgccgtcgac tcggacgacg ccggcgacgt cggccgccgc tcccgccccg
     2101 gtcgggcctc cgatcaaggc tccagcgcca gcacttgcca gcagagcggc cgtcatgcca
     2161 gcgattgcaa ttttgcgttg tctcatctga gtgttctcct caagggatca cggtcatccg
     2221 ctgccgttgg cttcggatgc cgatcagacg tcgaggggcc gatgtccgat gtgatcacga
     2281 tgcgccgcca acctcggagg ccccggagac gaccatggga gttcgatgag agtcgaaccc
     2341 aggtgatggc tgaaatcgtg gggaacgtgt cattgacgcc atcgacctga tggcgcacga
     2401 tcgtggcgat ggtcagttcc acgccgctgc agcccattca cgacgtcgtc gtgatcgcgt
     2461 acgacggcat ccagagcctc gacgtcaccg gcccctacga ggtgttcgcg ggtgcgaact
     2521 cggtgctcgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnaccgaccg accatcgtct
     2581 cgatcgacgg cggaccggtc accaccgaga gcgggctgca gatcctgacc acccccgcca
     2641 gcgacagccc cgacacaact cgcccggtcg acaccctgct gatccccggt ggcagcggtt
     2701 cccgcacggc gtgcaccgat ccgcgtctgg tcgcaacggt gaccgagttg gccgggcgtg
     2761 ctcagcgggt ggtcacggtg tgcaccggcg cgttcgtcgc cgctggtgcc gggctggccg
     2821 acggtcgcac cgtcaccacg cattgggcgc gcgcagccga actgcgccgc cggttcccga
     2881 gcgtcatggt cgacgccgac ccgatctaca tccgcgacgg cgacctgtgg tcgtcggccg
     2941 gggttacagc cggcatcgac ctggctctgg cactggtcga gcacgaccac gacgccgagg
     3001 tcgctcaggt cgtcgcccgc tggctggtga tgttcctccg ccggcccggt ggccagaccc
     3061 agtttgccgc gccggtgtgg accgaacgtg ccgtgacgac tccggtgcgc gccgcgcagg
     3121 agctggtcga cgccgaaccc ggcggcgatc accgggtcga cgcgctcgcc gcccgtgtcg
     3181 ggatgagccc tcgccacttc actcggttgt tcaccaccga gaccggtctc agtccggcgc
     3241 gctacgtgac ggaggtgcgc gtcgaggcag cccggcgagc cctggagtcg acagcggacg
     3301 ccgtcgaagt gatcgctcgc cgctgcggtt tcggcacggc ggagacgatg cggcgcacct
     3361 tcacccgtcg cctcggcctg tcgcccgatc agtaccgcca acgcttccgg caccagccac
     3421 cggggcatcg caccgatcac ccgaaccatc acgatcaccc gaaccatcac gatcacccca
     3481 accgtcacga tcacccgaac cgccacgaac ggagcaaccc atgaccatcc acgtcgccat
     3541 cccgctcttc ccacggctga ccgcgctcga cgcgatcggg ccgtacgagg tgctgcagcg
     3601 catccccgag atcgaggtga ccttcgtcgg ccacgagcgg ggcgaggtcc gcaccgagaa
     3661 cggactgctc ggcctgaccc gcgacgcgac cttcgaggag cgccccacac ccgacgtgat
     3721 cgtgtttccc ggcggcgtcg gcacgcggac cgtgcaacac gacgagcgtg tcctcggctg
     3781 gttgcgccac gcccatgcga cgaccacctt caccacctcg gtctgcaccg gatcactggt
     3841 gctcggcgcg gcggggctgc tcgacgggct caccgcgacc tcgcactggg cgacctaccc
     3901 cgaactggaa gcgaccggcg cgacgccgac cgcgcagcgg gtggtcgagc acctcgaccg
     3961 gcggatcatc accgccgccg gggtgtcgag cggcatcgac atggctctgc gcttggtcga
     4021 gctgctggtc gacgccacag ccgccgaagc agcgcagctg atgatcgagt acgacccgca
     4081 acctccgttc gacagtggct ccgttgccaa gtcgagccag gccgtggtcg aacgcgtcat
     4141 cgaatacgcc caactccgca gctgagaacc cgcgggttac cgnnnnnnnn nnnnnnnccg
     4201 ctcaggagcc aaccaccggc acatacgtca tccgacgatg ttgtgcacgg cgcggcgcac
     4261 ccgctgcagc gctgggaggt cgctacggct gacccgttcg agcagccgat cgaccggcac
     4321 tgtggcgaac tgattcgcag cgacgacccc ttcggcgatc ggtacgtacc acggccgttg
     4381 cgggccaggc gacgcgtcga gcaccggcgc gatgaccacg cggccagcca aacggtggaa
     4441 ccggtgatcc gacacgatgt acaccagccg ccgacgctcg tcgttgcggt cggcgcacca
     4501 gacgtctccg gcagacggtg tcaccccagc tcacccatcc acgcgtcggt ctcggcttcc
     4561 acgcgcaacc gatggtcgcg ttcggcggca gtctcaaccg caagcgccgc ctccgcagcc
     4621 gtccagaact gctgcgcctc gaaggcgtcg agcgcatgca cgacgacgtc ctccaacgag
     4681 tccccctcgg cgccgagcac acgcaaccga tcacgggtcg ccgtactgat cttcatcgtt
     4741 gtcaccccag ccatacccca agtatacgta cccggcaaca tgcccgctga gcgggtgcgc
     4801 tgggatcggt caacccagtg gggccagcgg gagggggatg cgggctgcgc cgagggcggt
     4861 gagggcggct tcgtccacgg tcacgcccag gcccgcgccg gtcggcacgg cgatggtcga
     4921 gccgcgcagg acgaacggct cggtgatgtc acggcgccag tagcgctcac tggccgagat
     4981 gtcggccggg aaggcgaagt tggccagtgc ggcgagggcg acgttggccg cccgaccgac
     5041 accggtctcg agcatgccgc cgcaccacac gggcacgcct cgctcggcac acgcatcgtg
     5101 gatgcgccgc gactccatca cgccgccgac ccgtccgatc ttgatgttga cgatcgagca
     5161 cgcgcccagg tcgatcgcca atcgggtcgt cgcgaccgac aagatcgact cgtcaaggca
     5221 gatcggtgtc gccatccgcc gcaccaatgc agcgtgggca gtccactcct cgggcgcgaa
     5281 cggctgctcg atcatcagca ggccataccg gtcgagcgac accagcaggt cggccacatc
     5341 gggcgtgtat ccggcattgg cgtcgacttg cagcacgaga tcgggatggg ccgcgcggac
     5401 ggcgcgcagc ggttcatggt cccaccccgg catgatcttg agcttcaccc gccggcttcg
     5461 gatccgtcaa ctgcttgtag gcgacgtagc cgtcgacctg cgcgaccagt tcgtcgacgg
     5521 tcggcgtgat gccgatcgac acacccacgt cgatgcgcgt tctcgtcgct ccgaggtact
     5581 gcgccaggct cacgtccgag gcgcgcaact cggcgtcgag gatcgccatc tccagccccg
     5641 ctttggccgc tgggtagccc ttgacatggc gcatcgccgc gccagcggtg acgcctgtca
     5701 aatcgcccgt gtcattcagc gccgcgaccg cgggcaccaa ccaccgttgc agcaccagta
     5761 ccgacgcatc ggtgaactcc tcgttgtaga acggctcggc gtgcgcggcg cactcgcccc
     5821 agccctcccc cacgtcggtg acgacgtgga ccnnnnnnnn nngcgtgtct cttgcgcgcc
     5881 gaacgaggtg cggaacgggc gcacgagcgg catcgccacc cgcaccaatt cgacatgatc
     5941 gatcttcaac gtcaactccg gtgccgcatg ttgcgcggcg gcgcgatctc cgcgacccac
     6001 accgcgtccg tcgtcgcctg cagcacaccg agctcatcga gcgatgcagc gggcgcaacc
     6061 gtcaccccgg cttcctgagc cgcgagacgg ggatcgtcgt gcgcgttcat gctgctggcg
     6121 cgtggggcag cgtctcggtc acccgtccat catcgcactc gacggactgc gcggcccacg
     6181 atcagcatcc gccgagtgtc accagcaaca gaccaagccc gggcgactac gcggtcgggc
     6241 caccgcgggc caggcggccc gggcgctcgc ccgtgtccgc gtcgttgcgg cggacggcga
     6301 cgccgttgac caacgtcgcc acgtaccccg tggacggctg caggatgcgg ctcgcgccgg
     6361 agggcaggtc gtagcgcacg aacggggcgt ggatcgtcaa ccggtcgagg tcgaccacgt
     6421 tgatgtcggc tcgcaggcct tcgacgagcc gaccgcggtc gttgaagccg tagaggtcgg
     6481 cgttgttggc ggtcatcttg gcgaccacgg tctcgaccgg cagcccatcg acccgatcac
     6541 gtgcccagtg ggtgaggtgg aacgtggtcg aggaacagtc cgagatgagg ttgacgtgcg
     6601 cacccgcgtc ggacagaccg gtcacggtgt tggggtcgag cagcatgtcc ttgcagacgt
     6661 cgaggttgcc gccgacgaag ttggagccga ggaccatgta aaagtcgttg ccgccgttgg
     6721 cagtcagcag gtcgtacatc agctccgcga catccagtcc acgggccatc gcctgggcga
     6781 ggaccgaccg atcgagcgag ggttcgtagt tcaccggctc ggtcagcggg aacgtgaccg
     6841 gcaatgcatt ggtgaacagc atcgccagac cccgcgccaa cccgccagca gggtcatcgg
     6901 tctcggtcag gatcgccgct ttgacctcgg gcttgtgcat ctccgccgcc cgctcggcca
     6961 ggccgaggtg ggccagcttc tggtacgtgg ggcggagcat gaacatgtgg tacgtgtcga
     7021 ggctggtcat caccgtgacc gagcggggga tgatctgggg tcggaacgcg agcgacccga
     7081 cggcagcatt ggccgcggcc gacgtgtcga gcatgcgccg ccagccgtcg ggatcttcga
     7141 acagctgtgc cacggtgaag gtgaccgggc ggcccgatgc ggcctgcacg gcacggagca
     7201 gttcgaactc ctcgctcatc gacgcccgct cgcccccgag gccgtcgagg cgcccgatcg
     7261 tgctggcgac gatcgcttcg aacacgccgt ggccgacgct gcccatcgcc tccgcgatgc
     7321 cgaccagttc gtcgacagcc gcaaaggtgc cgggtacggg cgtgcccgac ttggaccggt
     7381 gcccgatcgt gcgcgacgtg gagaagccga ccgcaccggc cgcgaacgcc tcgcgggtca
     7441 gccgggccat ctctgcgatg tcgtcggcgg tggcgtcttc gttggccacg ccgcgatcac
     7501 ccatcacgta gtagcgcagc gcgccgtggg cgagttgggc ggcgatgtcg accgcgtacc
     7561 ggcggccagc gagcacgtcg aggtagtcgg cgaacgactc ccacgatccc cagggcatgc
     7621 cgtcgaccag cgccgaaccg gggatgtcct cgaccccttc catcaggtcg atcagggttg
     7681 ccgttccgtc ggggcgcacg ggcgcga
//
LOCUS       DAORNF010000045         7703 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_7014_length_7703_cov_1.636114, whole genome shotgun sequence.
ACCESSION   DAORNF010000045 DAORNF010000000
VERSION     DAORNF010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 7703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7703
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7014_length_7703_cov_1.636114"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>928)
                     /locus_tag="PLV68_02960"
     CDS             complement(<1..>928)
                     /locus_tag="PLV68_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00231.1"
                     /translation="ACFAEAARKRAATADVIVVNTYLYGLHVGSGGVLLPEHDVVVID
                     EAHSLEDIMSDTVGVAINAGRFTSLAATVRRILDDPSTVGTIIDAGTALRDTLTGAVG
                     QRLPHPLPDAIAEALSNGRRRITDALGVLVAITTDVDDAKQRKLRAQTQAARLVEDID
                     RALTAGDGFVTFVSGRPDQPRLEVAPLDVGPTLREGVWNTHTAVLASATIPTSLAARV
                     GLGDDGVVHEDVGSPFDYEHNGLLYCALHLPDPRSAQFGSGVHDELAALIAAAGGRTL
                     ALFTSYKAMEAAAEAIGKRLPNPVLTQRDLPK"
     assembly_gap    929..1050
                     /estimated_length=122
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1051..1495)
                     /locus_tag="PLV68_02965"
     CDS             complement(<1051..1495)
                     /locus_tag="PLV68_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HRE00232.1"
                     /translation="MKPGSSTADVAATLARVTAGLAGREERPGQVRMAELVADAIRSK
                     THLVVQAGTGTGKSLGYLVPAVLAGKRVVVATATKALQDQLASKDLPFLQRQVDVPFT
                     WAMLKGRSNYVCLQRVREVTQSSQGQLELDDLAPTMRVEVKRLAEW"
     assembly_gap    1607..1698
                     /estimated_length=92
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1699..2165
                     /locus_tag="PLV68_02970"
     CDS             <1699..2165
                     /locus_tag="PLV68_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00233.1"
                     /translation="RPVQARRLPEHQPEELPCASPLTARVVLSRAGLGCARCGLALLA
                     LIRPTTPNSIGDTMVKKQSARASNPFQASLSPDDLSPANRLAFEIFAERRDLLPSIER
                     ILNAGLDDTTAATALGFDGKPFRSRTSDPGGDQCVDPRVAIRWATETESRGT"
     gene            2389..3384
                     /locus_tag="PLV68_02975"
     CDS             2389..3384
                     /locus_tag="PLV68_02975"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HRE00234.1"
                     /translation="MLAVAYFALVVANLGLLVRLGAAALVVVSSVALATGVLHDANHG
                     SFSRLRWLNRVLACSSDALGASSFLWRIQHNQLHHNHTNVVGFDADVELGPWARVAPA
                     QPWRPRYRWQHVYIWPLYGFLVIKNLLVSDLTSLVQGRIGHQPIRTRITAGVIVRIAL
                     GKCAHLAWAIVIPLMFNPWWAVLAFYMACSWTAGLILAVIFQVAHCVDAVEAVDAGAP
                     RRGQDFAAHQLRTTVDIASSGLVSGPLLRWIAGGLDHQIEHHLAPGLPHTIYPTLAAR
                     FRAALAHHDVGYRIHSGPIEAIRSHARWLRAMGAQPAIAPQVRGSRVFGDHHGRP"
     gene            complement(3341..4705)
                     /locus_tag="PLV68_02980"
     CDS             complement(3341..4705)
                     /locus_tag="PLV68_02980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00235.1"
                     /translation="MARNEQVDIPAPGWPAADPWGDAVAAFLSDLLDHSVEVTVHTDA
                     PDFTANGDRFPADPQLGADGRTRQFSVSTTRGEDGGDSAARFPPELEDLPGAALRALA
                     RVTQLAACGLPYPSVARALHDVVVAEVIADRLRRRSAGGVELDALVMTVAGTIDYFAE
                     LAATPLEGAPVTHGIVIASDPTGVEAPASTVAYPGRLHTRKRTPLLFDGTQSALVVDM
                     LGRALRGVERSTLPLMDPEPSRLETFDELPGLDGALTAAASAAFHGVGFYLRGDPSTW
                     IFDDGSPLFVRRGLQWKSIAFDSFTRTLAELGGTTEQIGQRIARSALRLSMQGRGAIL
                     AIATDAAALVDVTEPRDRAPVTGYHGDSVDDDLRQLLRLHDVSSPAGLARLARLDGAT
                     IVDTTGQVIAYGAIVRSTDSQTEGARSAAARTLSTAANVTLSVSHDGPITVYRGGRRI
                     LEIL"
     gene            complement(4943..5494)
                     /locus_tag="PLV68_02985"
     CDS             complement(4943..5494)
                     /locus_tag="PLV68_02985"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HRE00236.1"
                     /translation="MTTLTYYTATTLDGFIADPHDSLDWLLRQPHDDNGLLNYEDFIV
                     DIGAIVMGSTTYQWVRDHYPDEWLYTMPAWVLTSRELQPPAGSDVRFASGDVHPVYDD
                     MIAAADGKNLWVVGGGELAGRFADAGLLDEVIVSIAPVTLGAGRPILPRRLDLELLEV
                     GRNGALVAARYKVSGPLTEDRHP"
     assembly_gap    5641..5667
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5721..>5988
                     /locus_tag="PLV68_02990"
     CDS             5721..>5988
                     /locus_tag="PLV68_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013811228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HRE00237.1"
                     /translation="MSWLLLIIAGLLEVGWASVLPAAEGFTRPMPTVAFLVLLVASMF
                     LLAKASEQIPLGTAYAVWVGVGAVGAALVGIVVRHEPATAARMVF"
     assembly_gap    5989..6037
                     /estimated_length=49
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6118..>6346
                     /locus_tag="PLV68_02995"
     CDS             6118..>6346
                     /locus_tag="PLV68_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00238.1"
                     /translation="MTRTSTTTIRVSVDQRTRLRELADQRGERMAETLDAALETLRRD
                     EFYRQMAMAEQRLRADSVAWAEYSAERDEWLE"
     assembly_gap    6347..6454
                     /estimated_length=108
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6455..6719
                     /locus_tag="PLV68_03000"
     CDS             <6455..6719
                     /locus_tag="PLV68_03000"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HRE00239.1"
                     /translation="CVVVASDAYLRMRPTVVFAVPLTTTNREFPSHIPVPADPANGLT
                     RPSWVQVEQLRSVSSLRCSPALGNVGAVVTAQIHDVLAMLTGA"
     assembly_gap    6800..6939
                     /estimated_length=140
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6940..7460
                     /locus_tag="PLV68_03005"
     CDS             <6940..7460
                     /locus_tag="PLV68_03005"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HRE00240.1"
                     /translation="GQPGEEGDDPIHPPNLWEPYRSSRFKVGEDMYATIGIAREDKAG
                     RLAQFAKNLDFFGAPAAVFCFVDRIMGPPQWSDLGMFLQTFMLLAIEAGLDTCPQEAW
                     CVHEQAVSEFVGAPPEQRIFCGVALGHADHIVGAAGVAARRPRPSDQQFADRARTRGR
                     LRPLRLTLFPRP"
     gene            7539..>7703
                     /locus_tag="PLV68_03010"
     CDS             7539..>7703
                     /locus_tag="PLV68_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_465540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HRE00241.1"
                     /translation="MQGTVKTFNSEKGFGFISRPGAQDVFVHHSNIAGEGYKSLTEGQ
                     QVEFEVGPGRK"
BASE COUNT         1105 a         2385 c         2473 g         1202 t
ORIGIN      
        1 ttcttgggca ggtcgcgctg ggtcaggacc gggttgggca gacgcttgcc gatcgcctcg
       61 gccgcggctt ccatcgcctt gtagctggtg aagagggcca acgtccgtcc acctgcagcg
      121 gcgatcaggg cggcgagctc gtcgtgtacg ccgctgccga actgcgccga gcgcgggtcg
      181 ggcaggtgca gcgcgcagta cagcaggccg ttgtgctcgt agtcgaacgg gctgccgacg
      241 tcttcgtgca cgaccccgtc gtcgcccaga ccgacgcgag ccgcgagcga ggtcgggatc
      301 gtggcgctgg ccaacacagc ggtgtgggtg ttccatactc cttcgcgcag cgtgggaccg
      361 acgtcgagcg gcgcgacctc cagtcgtggc tggtcggggc gtcccgagac gaaggtgacg
      421 aagccgtcgc cggcggtgag cgctcggtcg atgtcctcga ccaaccgcgc tgcctgggtc
      481 tgggcgcgca gcttgcgttg cttcgcatcg tcgacgtcgg tcgtgatcgc gaccagcacc
      541 cccagcgcgt cggtgatccg ccgtcggccg ttgctgagcg cttctgcgat cgcatcggga
      601 aggggatggg ggagccgttg cccaaccgcg ccggtcaacg tgtctcgcaa cgccgtcccg
      661 gcgtcgatga tcgtgccgac ggtgctcggg tcgtcgagga tgcggcgcac ggtggcagcc
      721 aggctcgtga agcgcccggc gttgatcgcg acaccgacgg tgtcgctcat gatgtcttcc
      781 agcgagtggg cttcgtcgat cacgaccacg tcgtgttcgg gcagcagcac cccgccgctg
      841 ccgacgtgca acccgtacag gtacgtgttg accacgatga cgtcggcggt cgcggctcgc
      901 ttgcgcgccg cttcggcgaa gcaggcctnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cccattcggc caggcgcttg acctcgaccc
     1081 gcatcgtcgg ggcgaggtcg tcgagctcga gctggccctg cgaggactgg gtgacctcgc
     1141 gcactcgttg gaggcagacg tagttgctgc gccccttcag catcgcccag gtgaacggga
     1201 cgtcgacctg acgctgtagg aacggcagat ccttgctggc gagttggtcc tgcagcgcct
     1261 ttgtcgccgt ggcaacgacg acgcgtttgc cagcgagtac tgcggggacg aggtagccca
     1321 acgacttacc ggtgccggtc ccggcctgta cgacgaggtg cgtcttggac cggatcgcgt
     1381 cagcgaccag ttcggccatc cgcacctgcc ccggccgttc ctcccgcccg gccagccccg
     1441 cggtgacgcg cgcgagagtc gcggcaacgt cagcggtgga ggatcccggc ttcaccgtgt
     1501 cgagtgtggc agggcggggg agcagaacgg cggctcatga acaggcaggg gccgcagcgg
     1561 ctcgtccggc gacgttggtg acgcgtcgat gctgggcccg cggcacnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnngt cgccccgtgc aggctcgacg cctccccgag catcagcccg
     1741 aggagttgcc atgtgccagc ccgttgaccg cccgagtggt gttgtcgcgc gccgggctcg
     1801 gatgcgctcg ttgtgggttg gctctcctgg ctctcattcg acccacaaca cccaacagca
     1861 ttggagacac catggtcaag aagcagtctg cgcgggcatc gaacccgttc caagcctccc
     1921 tgtcacctga cgacctcagc ccggcgaacc gactcgcctt cgagatcttc gctgagcgcc
     1981 gtgatctcct cccgtcgatc gaacggatcc tgaacgccgg tctcgacgac accacggcgg
     2041 ccaccgcgct cggcttcgac ggcaagccgt tccgctcgcg cacgtccgac cccggaggcg
     2101 atcagtgtgt cgatccgcgg gtagcgatcc gctgggcgac tgagaccgaa tcgcgcggca
     2161 cgtgagccgg gtcagggcgg gcgagcgcac tcgcgacgcg gcgtggcgtc ggtcggacga
     2221 gaccgtcggc gccggaaccg aggaccggtg atgtcctcca ccccggttat cgagacggtc
     2281 cgtccagcgt tgtcgatcgc tgccatctgg cggtcggtgg gcccgacggt cgaggagttg
     2341 cgggccgccc ggcgcaggct gcacggcaag gcgatcgtga tcgcgtccat gctggcagtc
     2401 gcatacttcg cgctcgtcgt cgccaacctg gggctattgg tgcggttggg tgcggccgcc
     2461 ctggtcgtcg tcagttctgt cgcgcttgca acgggggtgt tgcacgacgc caaccacggg
     2521 tcgttctccc gtctgcggtg gctcaatcgg gtactcgcct gttcgtccga tgcgctcgga
     2581 gcgagctcgt tcctctggcg catccaacac aaccagttgc accacaacca caccaacgtg
     2641 gtcggtttcg acgccgacgt cgagctcggg ccgtgggctc gcgtggcccc cgctcagccg
     2701 tggcggccgc gttaccgctg gcaacacgtc tacatctggc ccttgtacgg cttcctggtg
     2761 atcaagaacc tgctcgtcag cgatctgaca tcgctcgtcc aaggacggat cggccaccaa
     2821 cccatccgca cacggatcac ggccggagtg atcgtgagga tcgcgctcgg caagtgcgcc
     2881 catctggcgt gggcgatcgt gatcccgctc atgttcaacc cgtggtgggc ggttctggct
     2941 ttctacatgg catgttcctg gacggccgga ttgatccttg cggtcatctt tcaggtcgcc
     3001 cactgcgtcg acgcggtcga ggccgtggac gccggcgccc cgcggcgtgg acaggacttc
     3061 gcggcccacc aactgcgcac gacggtcgac atcgcgtctt ctgggctggt gagcgggccg
     3121 ctgttgcgct ggatcgccgg cggactcgat catcagatcg agcatcacct cgcccccggc
     3181 ctgccgcaca cgatctaccc caccctcgcg gcccgattcc gtgctgccct ggcgcatcac
     3241 gacgtcggct accggatcca ctccgggccg atcgaggcca tccgctcgca cgcacggtgg
     3301 ctccgggcga tgggagcaca accggccatc gcgccgcagg tcagaggatc tcgagtattc
     3361 ggcgaccacc acggtagacc gtgatcggtc cgtcgtggga gacgctcagc gtcacgttgg
     3421 cggccgtcga cagcgtgcgc gcggccgccg agcgcgctcc ttcggtctga ctgtcggtgc
     3481 tgcggacgat ggctccatag gcgatcacct gtcccgtcgt gtcgacgatc gtcgctccgt
     3541 cgaggcgtgc cagccgagcc agcccagccg gtgaggagac gtcgtgaagg cggaggagct
     3601 ggcgcagatc gtcgtcgacg ctgtcgccgt ggtagccggt gaccggagcg cggtccctgg
     3661 gctcggtgac atcgacgagc gcagcggcgt cggtcgcgat cgcgaggatc gccccccgac
     3721 cctgcatcga caagcgcagc gcggaccggg cgatgcgttg cccgatctgc tcggtcgtcc
     3781 cgccgagttc ggccagcgtc cgagtgaagg agtcgaacgc gatcgacttc cactgcaggc
     3841 cgcggcgcac gaacaacggt gagccgtcgt cgaagatcca ggtgctgggg tcgccgcgga
     3901 gatagaagcc gacaccgtga aaggccgctg acgccgcggc ggtgagtgct ccgtcgagcc
     3961 cggggagctc gtcgaaggtc tcgagccgtg acggctccgg gtccatcagc ggcagcgtcg
     4021 accgctcgac gccgcgcagc gcccgtccga gcatgtcgac gaccagcgcc gactgggtgc
     4081 cgtcgaacag caacggcgtc cgcttgcggg tgtgcagccg tcccggatac gcgaccgtcg
     4141 acgccggtgc ttcgacaccc gttgggtcgg aagcgatgac gatgccgtgc gtcaccggtg
     4201 ctccctcgag tggtgtcgcc gccaactcgg cgaagtaatc gatcgtcccc gccacggtca
     4261 tcacgagggc atccagctcg acgccgcctg ccgagcggcg tcggagacgg tcggcgatca
     4321 cctcggcgac gacgacgtcg tgcagcgccc gtgccacgct cgggtacggc agaccgcaag
     4381 ccgcgagctg cgtcacccgt gccagcgctc gcagcgcggc gccgggcagg tcctccaact
     4441 cgggcgggaa ccgcgccgcc gaatcgccgc catcctcgcc gcgggtcgtc gacacgctga
     4501 actgacgcgt ccggccgtcc gcgccgagct gcggatcggc cgggaaccgg tcgccgttgg
     4561 cggtgaagtc gggcgcgtcc gtatggaccg taacctcgac gctgtggtcc aacaagtcgg
     4621 acaagaacgc ggccaccgcg tcaccccaag ggtctgctgc gggccatccg ggagccggga
     4681 tgtccacctg ttcgttccgg gccatgccgg agcctacggc tgaacccgac ccggccgccg
     4741 taggtgggaa catgtcatgg acgctcagcc gatcgcaacg ggagaagcaa ctggtcgatg
     4801 gaacccctca ccccagaccg acgacgcgtc tcgcgaggac cagcgtcgcc ggctcggaga
     4861 gccataccgg acatccattc cgactcagtc gttccgtcga agacaagctc gctttcgtcg
     4921 atgacttcgt gcgcgacgtg gatcagggat ggcggtcctc ggtcagggga ccggacacct
     4981 tgtagcgggc ggcgacgagt gctccgttgc ggccgacctc gagcagttcg aggtcgagcc
     5041 gtcgcggcag gatcggtcgt cccgcaccga gcgtcacggg cgcgatgctg acgatcacct
     5101 cgtccagcag ccccgcatcg gcgaaccggc cggccagttc gcccccaccg acgacccaca
     5161 ggttcttgcc gtctgcggcg gcgatcatgt cgtcgtagac cggatgcacg tcgcccgacg
     5221 cgaaccgcac gtcggaacct gcgggcggct ggagttcgcg actcgtcagc acccaggcag
     5281 gcatcgtgta cagccactcg tccggatagt ggtcgcgcac ccactggtag gtggtggaac
     5341 ccatgacgat cgcgccgatg tcgacgatga agtcctcgta gttcagcaga ccgttgtcgt
     5401 cgtgcggttg gcgcaggagc cagtcgagcg agtcgtgggg atcagcgatg aagccgtcga
     5461 gagtggtcgc ggtgtagtag gtcagcgtgg tcatgtgtcc cccagcaagc ggtctgccga
     5521 ttcggcgatc ctcacccgcc cggcctgtcg gacacggtag ggcggcgaag agatcgcctg
     5581 agcggatgac ggccgcgaga ggctgtgatg ggcgtgttgc gctgtgttcg atagcgtgtt
     5641 nnnnnnnnnn nnnnnnnnnn nnnnnnngcc gacgagggtg tccgtaccgg caggtgcacg
     5701 acggactcag agagacggcc atgtcctggt tgctgctgat catcgctggg ttgctcgaag
     5761 tcggttgggc atcggtgtta ccggctgccg agggtttcac gcggccgatg cccacggtcg
     5821 cgttcctcgt gctgctcgtc gcgagcatgt tcctgctcgc caaggcatct gagcagattc
     5881 cgctcggaac cgcgtacgcg gtgtgggtgg gtgtcggggc cgtcggtgct gcgctggtcg
     5941 gcatcgtggt ccgtcacgaa ccggccaccg ccgcgcggat ggtgttccnn nnnnnnnnnn
     6001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngac gacaggcgcc cattgaggag
     6061 aggcacctct ggaggggcag cactgcctcg atgtagacac gggtgtagac tgaacgtatg
     6121 acacggacgt cgacgaccac gattcgggta tccgtcgatc agcgcacgcg tctgcgcgag
     6181 ttggccgatc aacggggtga gcggatggca gagacgctcg atgctgcact ggagaccttg
     6241 cggcgcgacg agttctaccg ccagatggcg atggccgagc aacgactccg ggccgattct
     6301 gttgcctggg cggagtactc cgcggagcga gacgagtggc tcgaggnnnn nnnnnnnnnn
     6361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnatgcgt ggttgtcgcc agcgacgcct
     6481 acttgcggat gcgcccgacc gtcgtgttcg cggtgccgct caccaccacg aaccgggagt
     6541 tcccgagcca catcccggtc ccggcggatc ccgcgaacgg cctgacgcga ccgagttggg
     6601 tccaagtcga gcaactccgg tccgtctctt cgctgcggtg cagtccagcg ctcggcaacg
     6661 tcggcgccgt ggtcaccgcg caaatccacg acgtgctcgc gatgctgacc ggcgcctgac
     6721 tcgaccgcgc gcggtgcatc acgacgcgtc agactgagcg gcgatgaagg tgaccgatgc
     6781 tgtcaatgcc cgccgttcgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc cggacagccg ggcgaagagg
     6961 gggacgaccc catccatccg ccgaatctct gggaaccgta tcggtcgtcg cggttcaaag
     7021 tcggcgagga catgtacgcc accatcggga tcgcccgcga ggacaaggcg gggcgcttgg
     7081 ctcagttcgc caagaacctc gacttcttcg gcgcgcccgc ggcggtgttc tgcttcgtcg
     7141 atcggatcat ggggccgccg cagtggtccg acttgggcat gttcctgcag acgttcatgc
     7201 tgctggcgat cgaggccggg ctggacacgt gcccgcagga ggcgtggtgc gtgcacgagc
     7261 aggccgtcag cgagttcgtg ggcgcaccac ccgagcagcg catcttctgc ggtgtggccc
     7321 tcggccacgc cgaccacatc gtcggcgcgg ccggtgtagc ggcacgccga ccccgaccat
     7381 ccgatcaaca gtttgcagac cgtgcgcgaa cccgtggacg tcttcgcccg cttcgtctga
     7441 cactgttccc gcggccgtga gggaggatgt tggcccgcgg acccgtaggg tccatggctg
     7501 acgatgccat cggtgtcgtc gaaacgaaag cagacaatat gcaaggcaca gtgaagacct
     7561 tcaacagcga gaagggcttc ggtttcattt cacgtcccgg cgcccaggac gtgttcgtcc
     7621 atcactcgaa catcgcgggt gaaggctaca aatccctcac cgagggacag caggtcgagt
     7681 tcgaggtcgg tcccggccgc aag
//
LOCUS       DAORNF010000046         7693 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_7031_length_7693_cov_1.720607, whole genome shotgun sequence.
ACCESSION   DAORNF010000046 DAORNF010000000
VERSION     DAORNF010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 7693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7693
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7031_length_7693_cov_1.720607"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>305
                     /locus_tag="PLV68_03015"
     CDS             <1..>305
                     /locus_tag="PLV68_03015"
                     /inference="COORDINATES: protein motif:HMM:NF016944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HRE00242.1"
                     /translation="VLGIDAAELSPLELISAILCAPVDLLWNGGIGTYVKASTESNAE
                     VGDRGNDAIRVNGADLRCRMVGEGGNLGFTQRGRVEYALAGGLINTDAIDNSAGVDC"
     assembly_gap    306..327
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <328..1642
                     /locus_tag="PLV68_03020"
     CDS             <328..1642
                     /locus_tag="PLV68_03020"
                     /inference="COORDINATES: protein motif:HMM:NF016944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HRE00243.1"
                     /translation="EVNIKILLNACVAAGELTVPDRNEFLASMTDEVAELVLDDNQAQ
                     TLALVMARKQSLAMANVHARYIHALESEGSLDRGLEFLPTDRQLAERQASGIGLTTPE
                     FAVLLAYTKNVDAQEILASALPDDPYLRDDLVRYFPVPMRERYATEIERHQLRREIIT
                     TQIVNQMVNLSGTSFAHRSVEQSGADITDIARAWIAARDITGLPALWQAIDELGADAS
                     GGAVKLDVRTDLLLDARRMTERVVLWLLRHRRAPIDLVSAVEHFRDPLALLARRYPDL
                     MTSQLKARIHSVWAGRLAAGVPEGVAERASVWPFLHTAFDVIDIAHRAEADLVATATL
                     YWQVFDRLDALWLWDGIGGLPRSTRWETQARSALRDDLASGLAGLTEEVLRSALGLDA
                     WLVANEASVERVSALFAEVRRGDTVDLTTLTVGLRQLRNLALATH"
     gene            1724..>1945
                     /locus_tag="PLV68_03025"
     CDS             1724..>1945
                     /locus_tag="PLV68_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HRE00244.1"
                     /translation="MSAELSSSSERPVRLRFAPSPTGFFHVGGARTALYNWAIAHRVG
                     GTFVLRIEDTDEARNRPEWTQGIIDALAWI"
     assembly_gap    1946..2089
                     /estimated_length=144
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2090..>2477
                     /locus_tag="PLV68_03030"
     CDS             <2090..>2477
                     /locus_tag="PLV68_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HRE00245.1"
                     /translation="ERARAAGKPGYDGFSRDRGLGPGPGRVLRFRVPEGTTVVHDLVR
                     GEVSFDNANIEDFVLLRGNGAPMFLLANVVDDITMRISHVVRAEEHLPNTPKQQMLWR
                     ALGEQPPVWAHVPVLVNEQRKKLSKRR"
     assembly_gap    2478..2487
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2488..3281
                     /locus_tag="PLV68_03035"
     CDS             <2488..3281
                     /locus_tag="PLV68_03035"
                     /inference="COORDINATES: protein motif:HMM:NF039902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HRE00246.1"
                     /translation="KVAVEQYRAEGYLADAMINYLMTLGWAPSGDTEIVPWSQIVADF
                     RLEDVNHSPAFFDIKKLTAFNGEYIRSLPVDQFVAACAPFLTSSPAAETDGARDAGAA
                     GADTAGVGAVADVAAVGSGVAWPSERFDPVVFAAIAPLVQTRVGRLDEVPAMVDFLFL
                     ADPVIDDDAFAKAFGTAAALDIVRDSIVACETIEWSHGEIKEVVDAIGTGYGLKLGKT
                     QAPIRVAVTGRSVGPPLFEAIELLGRDETLRRLRAALTRAEAAPE"
     gene            3446..3991
                     /locus_tag="PLV68_03040"
     CDS             3446..3991
                     /locus_tag="PLV68_03040"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HRE00247.1"
                     /translation="MNLAQVVIVGRSDQARRVDAIVVMGAAQYDGRPSPQLQARLDHV
                     VDLWNRGLAPLVVVTGGKQPGDRFTEAGASATYLVEHGVDAAAIVAEDEGRSSWQSLE
                     AAAVLLRERDVTSVLLVSDPFHSLRIRLMAQDLGFTAYVSPTRTSPVRGTSAFVKQLK
                     EAGGVAVGRIIGFDRLLSITG"
     gene            4035..4106
                     /locus_tag="PLV68_03045"
     tRNA            4035..4106
                     /locus_tag="PLV68_03045"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4068..4070,aa:Gln,seq:ctg)
     gene            4210..4282
                     /locus_tag="PLV68_03050"
     tRNA            4210..4282
                     /locus_tag="PLV68_03050"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4244..4246,aa:Glu,seq:ctc)
     assembly_gap    4530..4635
                     /estimated_length=106
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4774..5052
                     /locus_tag="PLV68_03055"
     CDS             4774..5052
                     /locus_tag="PLV68_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00248.1"
                     /translation="MGLYWRDCFARPAKRGRDRDRVEGGVQPSGLVVQANATIGRPPA
                     DDHQLLAGRDLLEAIGRRYSCLVLRSIGFIDLDLTAVLHEVGDHLIRR"
     gene            5498..5812
                     /locus_tag="PLV68_03060"
     CDS             5498..5812
                     /locus_tag="PLV68_03060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00249.1"
                     /translation="MTVLDLDQLPSDPLDALRELSRRESELGRLRREVIGSARAAGAT
                     WEEVGAALGMSRQAAWEYYSRATRARLEQLAADESDLSDDAAMELAVDEVKAARRERR
                     RA"
     assembly_gap    6313..6342
                     /estimated_length=30
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6730..6739
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6954..>7693
                     /locus_tag="PLV68_03065"
     CDS             6954..>7693
                     /locus_tag="PLV68_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00250.1"
                     /translation="MFGKKRAYEARLAQARAEHDAEVLLWEAQVAALPAQQKQLDDAY
                     AAAEHQRGAQLAAARAEYDEQCEQRETEVAEANQRLDQLIAHLGYEVEDAVQEYVGIV
                     LGNSVYPESFPVEHDFEFDSAHHELILRVSLPEPTAVPITKAYKYSKAADDITSTTIT
                     QKEQKDRYANAVAAVALRTLHEVFEADRAGRIHTISLTVSTAALDQGTGRMVDVPLVA
                     VASDRATFEDIDLAKVVPVATLAHLKESV"
BASE COUNT         1184 a         2382 c         2504 g         1301 t
ORIGIN      
        1 aggtgttggg tatcgacgcc gccgaactgt cgccgctgga gctgatctcg gccatcctgt
       61 gcgcgccggt cgacctgctg tggaacggtg gcatcggtac ctacgtcaag gcatcgaccg
      121 agtccaacgc cgaggtcggc gatcggggca acgatgcgat ccgggtcaac ggtgccgacc
      181 tgcgctgccg catggtgggc gagggcggca acctcggctt cacgcagcgg gggcgagtgg
      241 aatacgcgct cgctggggga ctgatcaaca ccgacgcgat cgacaactcg gccggggtcg
      301 actgcnnnnn nnnnnnnnnn nnnnnnncga ggtcaacatc aagatcctcc tcaacgcctg
      361 cgtcgccgcg ggtgagctca ccgtgcccga ccgcaacgag ttcctcgcct cgatgaccga
      421 cgaggtggcc gagttggtgc tcgacgacaa ccaggcccag acgctcgcgt tggtgatggc
      481 gcgcaagcag agcttggcga tggccaacgt gcacgcccgc tacatccacg cgctcgagtc
      541 cgaggggtcg ctcgatcgtg gtctcgagtt cctgcccacc gatcgtcagt tggccgagcg
      601 ccaggcgtca ggcatcggcc tgaccacccc cgagttcgcg gtgctgctgg cctacaccaa
      661 gaacgtcgac gcgcaggaga tcctggccag cgcgctcccc gacgatccgt atctgcgcga
      721 cgacctcgtc cgctacttcc cggtgccgat gcgcgagcga tacgcaaccg agatcgaacg
      781 tcaccaactg cgccgggaga tcatcacgac gcagatcgtc aaccagatgg tcaatctgtc
      841 gggcacctcg ttcgcccatc gcagcgtcga gcagtcgggc gccgacatca ccgacatcgc
      901 tcgcgcctgg atcgcggccc gtgacatcac cggactgcca gcgctgtggc aggcgatcga
      961 cgagctgggt gccgacgcgt ctggcggcgc ggtcaagctc gacgtgcgca ccgacctgtt
     1021 gttggatgcc cggcgcatga ccgagcgggt ggtgttgtgg ctgttgcgcc atcgtcgggc
     1081 cccgatcgat ctggtgtccg cggtcgaaca cttccgcgat cctttggcgt tgctcgcgcg
     1141 gcgctatccc gatctgatga cgtcgcagct gaaggcacgc attcactcgg tgtgggcggg
     1201 acgtctcgcc gcgggcgtgc ccgagggggt ggccgagcga gcgtcggtgt ggccgttcct
     1261 gcacaccgcc ttcgacgtga tcgacatcgc ccatcgcgcc gaggccgatc tcgtcgccac
     1321 ggcgacgttg tactggcagg tgttcgatcg tctcgatgca ctgtggttgt gggacgggat
     1381 cgggggcctg cctcgttcga cgcgatggga gacccaggcc cgttcggcgt tgcgtgacga
     1441 cctcgcctct gggctggcgg gcctgaccga agaggtgctc cgttcggcgc ttggcctcga
     1501 tgcctggctg gtggccaacg aggcttcggt cgagcgggtc agtgcactgt tcgccgaggt
     1561 ccgccgcggc gacacggtcg acctcaccac gctcacggtg ggactacgtc aactgcgcaa
     1621 cctcgccctc gccacgcact gagcgatcga tccggcggtg ctgcgttccg gcgcggcaac
     1681 cgcctgcact ctcgatcggt tccgtgcgta gcctggcgac atcatgtccg ctgagttgtc
     1741 ttcttcatcc gagcggcccg tccgcctgcg gttcgccccc tcgccgaccg gctttttcca
     1801 cgtcggcggg gcgcgcaccg cgctgtacaa ctgggccatc gcccaccgcg tcggaggcac
     1861 gttcgtgctg cgcatcgaag acaccgacga ggcccgcaac cgacccgagt ggacgcaagg
     1921 catcatcgac gcgttggcct ggatcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng agcgggccag
     2101 ggctgctggc aagccgggtt acgacgggtt ctcccgcgat cgcgggttgg ggccggggcc
     2161 ggggcgtgtc ctgcgcttcc gtgtgcccga ggggaccacg gtcgttcacg acctggtgcg
     2221 cggcgaggtc agcttcgaca acgccaacat cgaggacttc gtgctgttgc gcggcaacgg
     2281 cgcgccgatg ttcttgctcg ccaacgtggt cgacgacatc accatgcgga tcagccacgt
     2341 ggtgcgggcc gaggagcact tgcccaacac gcccaagcag cagatgctgt ggcgggcgct
     2401 gggcgagcag cccccggtgt gggcgcatgt gccggtactg gtcaacgagc agcgcaagaa
     2461 gctgtcgaag cggcgggnnn nnnnnnnaca aggtcgccgt cgagcaatac cgcgccgagg
     2521 ggtacctcgc cgacgcgatg atcaactatc tgatgacgct cgggtgggca ccgtcgggtg
     2581 acaccgagat cgtgccgtgg tctcagatcg tggccgactt ccgcctggag gacgtcaacc
     2641 actcacccgc gttcttcgac atcaagaagc tcaccgcctt caacggtgag tacattcgct
     2701 ccctaccggt cgaccagttc gtggcggcct gtgctccgtt cctcacctcg tcgcctgccg
     2761 ccgaaacaga cggagctcgc gacgcgggcg ctgctggggc cgacactgct ggcgtaggcg
     2821 cagttgccga tgttgcggcg gtcggtagcg gcgtggcgtg gccgagcgag cgattcgatc
     2881 cggttgtgtt cgccgcgatc gcgccgttgg tgcagacacg ggtcggtcgc ctggacgagg
     2941 ttccagcgat ggtcgacttc ctgttcctgg ccgatcccgt catcgatgac gacgcgtttg
     3001 ccaaggcgtt cggtactgcc gccgcgctcg atatcgtccg cgactcgatc gttgcatgtg
     3061 aaacgatcga gtggtcgcat ggcgagatca aggaggtggt cgacgccatc ggtacgggct
     3121 acggattgaa gctcggcaag acccaggcgc cgatccgggt cgcggtgact ggccgcagcg
     3181 tcggcccgcc gctgttcgaa gcgatcgagt tgctcggtcg cgacgaaacc ctgcgccgcc
     3241 tgcgcgccgc cctcacccgc gccgaggccg cccccgagtg aacgacgggc gccaggtcat
     3301 cgtggcttcc agcccacagg cggctgtcga tgtcgggatc gacagcgttg gtgcaagtcg
     3361 gcagagacgg tggtggcatc cgtgccgggc gtggtggtgg cgcgttccgc tggcggtgct
     3421 ggccgctggg ttgggctact acctggtgaa cttggcgcag gtggtgatcg tcgggcgtag
     3481 cgaccaggcg cgtcgcgtcg atgcgatcgt cgtgatgggg gccgcgcaat acgacggtcg
     3541 tccctcgccg caacttcagg cccgcctcga ccacgtggtc gacctgtgga accggggcct
     3601 ggccccgctg gtcgtcgtca cgggcggcaa gcagcccggc gatcgcttca ccgaggccgg
     3661 tgcgtcggcg acctatctcg tcgagcacgg tgtcgatgcc gccgcgatcg tggccgagga
     3721 cgaggggcgc tccagctggc aatctctgga agccgcggcc gtgctgctgc gcgagcgaga
     3781 cgtcaccagc gtgttgctcg tcagcgaccc gtttcactcg ctgcggattc gtctgatggc
     3841 gcaggacctc ggcttcacgg cctacgtgtc gcccacccgt accagccccg tgcgcggcac
     3901 gtctgccttc gtcaagcagc tcaaggaagc tgggggagtg gccgtcgggc ggatcatcgg
     3961 tttcgatcgt ctgctgtcga tcaccggctg acgccgatac actgcttccc gccttcgggt
     4021 gccttcgggc cacttgggga atggtgtaat tggcaacaca gcagtttctg gtactgttat
     4081 tcagggttcg agtccttgtt ccccagctca gccggccggt cgcttcagcg gccggccgtt
     4141 tgcgttccag ctctggaacc cggttggggc cggtgcgcgc cgttgctaga ttggcccagc
     4201 tacttgcacg gcccgttcgt ctagtggcct aggacaccac cctctcaagg tggcggcacg
     4261 ggttcgaatc ccgtacgggc tgcaacgcag aacccctggt cacggcgtcg accgaggcca
     4321 ggggttttgg cttagtgagg ctgactgtca gccagcttca cggaggtccg agatgatcca
     4381 gcaacggggt cgcaggtggc gagtggtcgt ccaggcccgg ccgaatccgg tgccgtacgt
     4441 tcggctgtcg caagcgctcg atgactgagc atgcggctcg tcgtccgagt gggtcgagat
     4501 caccaggctc accgatacgg cccgtacgcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4621 nnnnnnnnnn nnnnnctgcg tgtagccgta ccgatcgttc gtaaccctcg ttgcgcgcac
     4681 tgcgtacccg cggcgttcgg cctcggtcgc caatgcttgc gtgagaagga gcgctcgacg
     4741 aacgtgagcg cggtcgatca ggaacggacg tgattgggcc tgtattggcg cgactgcttc
     4801 gcgcggccgg cgaagcgtgg tcgggaccgg gatcgggtcg agggcggcgt tcaaccaagc
     4861 gggctcgtcg tccaggcgaa cgcgacaatc ggtcgaccac cggccgacga tcaccaactg
     4921 cttgccggcc gggaccttct tgaagcgatt ggccgccgct actcgtgcct cgtacttcgc
     4981 tcgatcggct tcatcgacct tgatctcacc gccgttttgc acgaggtcgg cgatcatctg
     5041 atccgtcgat gatggcggct tcggtcgggg gtagtccgcc gcgatttggg cgcgggcttc
     5101 tggtgtggca gggcaggtgg gatcgcgatg tgcgttgcga ctgtgggtcg aatcccggcg
     5161 ccggcctgtt ggagacgttg ccgaccgagc tcggtcgccg ccgcgctcca tcgaccctcc
     5221 ttcttcgaga cgaccaccag ttgacggttc tgcagcgcga ccgcggtgat cttgtacgag
     5281 tcgcccgtca tcacgccgtc tggacagccg tcgatgatcc accgcagtac ctcgacctgt
     5341 cggacgttca gcggagccaa ctttcttgcc actgagcctg tgtagccact gcgccgagag
     5401 cgatcacaat gcggtgagca cgatcgccga accgccgcat cgctcggaga cgggcaatcc
     5461 ttgcgcgact gctatttgtc aggatatcct gacagacgtg acggtgctcg acctcgacca
     5521 gttgcccagt gatccgctcg atgcgctgcg ggaactgtcg cggcgcgagt cggaacttgg
     5581 ccgactgcgt cgtgaggtga ttgggtcggc gcgtgccgct ggtgcgacgt gggaggaagt
     5641 cggtgccgcg ctcgggatgt cgcggcaggc agcctgggag tactactcgc gagcgacgcg
     5701 tgcgcgtctc gaacaactcg ccgccgatga gtccgatctg tccgacgatg ccgcgatgga
     5761 gttggccgtc gacgaggtga aggccgctcg ccgggaacgc cgccgcgcgt gaccatccgc
     5821 gcggtggtcg acgccaacgt cttcgtgtcg gcggcgatcc agcgagggcc gtcacaccgc
     5881 atcgtgcagg cctggcttgc cagccagcca tttgagctcg tcatcagcga aggcgtcctg
     5941 gccgaggtcg acgacgtcct gcaccggccg cgctggcaaa gcggatcaag tctgaggacg
     6001 cgaccgactt cgtcacgtcg atccgtacgc tcgcagacct cgtcgacgac ccgaccgacg
     6061 taccgccgga gacgcgcgat cccgacgacg actatctcat cgcgctggcg aaggcgaacg
     6121 atgcgaccgt catcgtgacc ggagacctcg acctgctgga gtggaagggt tcaccacccc
     6181 gcacactccc gcctgcagag ttcgagcaga tgctgggcgc gacgtaggtc aagggaccgt
     6241 cttcgggccc cgggatgtct cgtggagtga tgaggcgatc gttcggcttc cgcgcgagac
     6301 ctaagcgtca ccnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngatggtcg gatcgacccg
     6361 cgtcgctcgt cgcccctcag acaccccccg cttcccggtg gggctgcctg ggctggctac
     6421 cgttgggatg gacacggggc tcgccgatca cggctgttgc gagtttttag caaaggggac
     6481 gatgacagat ggccaagaag cgcgggttct tagcggagct caaccaccag caacagcagg
     6541 ccgccaagcg tcgtgcttcg gcccagcgtc aagcagtcca agcccaggct agggctgtgc
     6601 ggagtgccga gcaggcgagg cgccagtggg aacgtgccca ggctcaggag ttacgggccg
     6661 cgacagccga cgcgaagcgt gcccagcagg aagcccaacg actgtacgta gaggcccgtg
     6721 aggctgaggn nnnnnnnnnc ttggcggtca ggtatgagga gattgatggg atcctggcgg
     6781 cgaccctcaa cgttgacgac ttcgtcgacc ttgaaacgct tcgtcaggtc gccgaacacc
     6841 cacggttcga gcctggccag cttgcgttcc cggcaccggc acctgcgcga cttcaagcgc
     6901 cgcctgcacc cgaatgggtg cccccacagg gtgagccgaa aggactcgtc ggcatgttcg
     6961 gcaagaagag ggcgtacgag gctcgcctcg cccaggcccg cgccgagcac gacgcagagg
     7021 tgctgttgtg ggaagcgcag gttgctgcac tcccagccca gcagaagcag ctcgacgacg
     7081 cgtacgcagc cgcggaacac cagcgtgggg cccaattggc cgcagcgaga gccgagtacg
     7141 atgagcagtg cgagcaacgt gagactgagg tcgcagaggc gaaccagcgg ctcgaccagt
     7201 tgattgcaca cctgggctac gaagtggaag acgccgtcca ggagtacgtg ggcatcgttc
     7261 tcggtaactc ggtgtacccc gagtcattcc ccgtcgagca cgacttcgag tttgactctg
     7321 cacaccacga actcattctg cgcgtctcat tgcctgagcc aaccgccgtt ccaatcacca
     7381 aggcatacaa gtactcgaag gctgctgacg atattacgtc gacgacgatc acgcagaagg
     7441 agcagaagga ccgctacgcc aacgcagtcg ctgccgtcgc attgcgcacg ttgcacgagg
     7501 tgttcgaggc ggaccgcgcc ggcaggatcc acacgatctc gctcacggtc tctacggccg
     7561 cgctcgacca gggcaccggg cgcatggtcg atgtgccgct ggtagcggtg gcatcggatc
     7621 gggccacgtt cgaggacatc gacttggcga aggtggtgcc ggttgcgacg ctcgcccacc
     7681 tgaaagagtc tgt
//
LOCUS       DAORNF010000047         7681 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_7041_length_7681_cov_1.847233, whole genome shotgun sequence.
ACCESSION   DAORNF010000047 DAORNF010000000
VERSION     DAORNF010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 7681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7681
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7041_length_7681_cov_1.847233"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1060)
                     /gene="trpB"
                     /locus_tag="PLV68_03070"
     CDS             complement(<1..1060)
                     /gene="trpB"
                     /locus_tag="PLV68_03070"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HRE00251.1"
                     /translation="MSEPSDTGRFGEFGGRYVPETLVPACMELEAAFREAWNDPGFRG
                     ELDGLLRDYAGRPSILTECHNLGRALGIRLLLKREDLNHTGSHKINNVLGQALLAKRM
                     GKTRLVAETGAGQHGVASATAAALMGLSCKVYMGAVDVERQALNVFRMRLLGAEVEAV
                     NSGSRTLKDAVNEAMRDWVATVGESHYCLGSVMGPHPYPWMVREFHRVVGSEAHEQCV
                     ALTGDVPDAVVACVGGGSNAIGIFSGFVDTRSRLIGVEPAGGAAVARGVPGVVHGMRS
                     YLMQDEYGQVLEAESISAGLDYPGVGPEHSYLASIGRAEYPSVTDAEVIEAFQVLART
                     EGIIAALESVHALAWVLRE"
     gene            complement(1152..1808)
                     /locus_tag="PLV68_03075"
     CDS             complement(1152..1808)
                     /locus_tag="PLV68_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HRE00252.1"
                     /translation="MFVKICGITNEDDALFAVAMGADAVGFVFAPSTRQVAAQQVYDI
                     TRRLPPEILTVGVFRDDHPSRVIDMVHKSGVKAAQLHGHESPSDVAEVARHVRWVIKA
                     VAAGSTDARRADQFGTDLVLVDGSTPGSGRMFDWSLADEVSGAVRLILAGGLDPDNVA
                     AAVAQVRPWGVDVSSGVERSPGKKDALKVKAFIERARAAAPTPYIGPDNLPYDWADDE
                     "
     gene            complement(1868..2656)
                     /gene="trpC"
                     /locus_tag="PLV68_03080"
     CDS             complement(1868..2656)
                     /gene="trpC"
                     /locus_tag="PLV68_03080"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HRE00253.1"
                     /translation="MATYLDRIVARHREVAATDDRPLGALIERAASAAPIRPFGTALR
                     RASEHGELAVISEIKRRSPSKGALNVGLDPAEMAQRYAAGGAAAMSVLTDVEFFGGSV
                     ADLQAARAAVTLPVLRKDFTVSARDVCDARLMGADCVLLIAAALGRDELVALHRIADE
                     IGIDVLVEIHDEPELDVALASGATIVGVNQRDLVSFQVDHDRAVRMAAVIPDDVVKVA
                     ESGVRGADDARSLQAAGYHAVLVGETLVTAADPAATIGALRGVA"
     gene            complement(2656..2970)
                     /locus_tag="PLV68_03085"
     CDS             complement(2656..2970)
                     /locus_tag="PLV68_03085"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HRE00254.1"
                     /translation="MANVAIIAKIPAKPGMRDQLIEALQVGIQNVQSEPGTLQYIMHK
                     DANDPDLVWFYEFYDSQESFVHHGSTDGIKAMGMASREFAAGRPELIFLEPVAGKGTP
                     GS"
     gene            complement(3020..>5800)
                     /locus_tag="PLV68_03090"
     CDS             complement(3020..>5800)
                     /locus_tag="PLV68_03090"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRE00255.1"
                     /translation="QPLPDGPGAIDFDDVSFGYTTTEPILDGLSLRVEAGEVVALVGG
                     SGSGKSTLTALLPRFYDVHSGSIRIDGTDVRDATLASLRRQVGVAFEESFLFSDSIHA
                     NIAYGQPDATDAEVREAARIAGAAGFVEALPDGYATVVGERGMTLSGGQRQRIALARA
                     IVTNPRILVLDDATSAVDSATEEAIHAALHDVMAGRTTLLVAHRASTVRLADRVVVIE
                     NGRVLDTGTHDELLARSARYRDLLGDGLGSAAVATATTSDPGDVERDDAGLDDVGITA
                     SAWPVRARPAAVASAITTRTVIGPPGGGNFGANLAPTSDLLAALGRLPAADDEPGIDV
                     IGEASAELANGTAGNGAGAFRILRFARRWRRWLAIGLALVATDAVLTVLGPAIMRFGI
                     DHGISAGDGAVLRGAALVFVIVGLSDWCVTWVGAVVTGRTAERMLLSLRMRIFGHLQR
                     LSLDYYDREMDGRVMTRMTTDVEALSQLVQTGLVNAVVGMATCLGVAVYLAIQSWPLA
                     LVAASALVPLAGATWWYQRHSSAAYRSARDAIANVNADLQENLSGVRLTQASGRERVN
                     ADRFRATSGTYLAARLRAQKLLALYFPGVGLLADLAAVAVLGAGAAFVTDGITTPGVV
                     IAFVLSLNVFFAPIQQLSQVFDSWQQASASTAKITELLQTPTATPPPAQPIDPGRLRG
                     EIELVDVHLVYPGARNEALSGVDLHIAPGRTVALVGETGAGKSTVVKLLARFHDPTAG
                     MVRIDGIDLRDLDLSAYRRQLGVVPQEPFLFTGTIRDNIAYGRPDASDAEVEQSARAV
                     GAHGFIAGLADGYLTTVSERGRSLSSGQRQLIALARAQLVDPVILLLDEATANLDLAA
                     EATVRSAMDAVSANRTTVLVAHRLATARAADEIVVIDAGRVLERGSHDDLLAEGGRYA
                     QMWAVHDGLSTV"
     assembly_gap    5801..5829
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5830..>6422)
                     /locus_tag="PLV68_03095"
     CDS             complement(<5830..>6422)
                     /locus_tag="PLV68_03095"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HRE00256.1"
                     /translation="VSRASGDLGLIQQLLAFLPLMLGNLIMLVISIVAMVWLSPLLSL
                     VMVAALPLMLVTALRLRATVFPATWDAQQRAAEVAGVVDEAVSGVRIVKGFGAEDREI
                     ARLADTATALYRARVRLVRLQARATSTLAAIPMLAQAAVLGVGGWLALHDRLSVGTLL
                     AFATYLVQMTAPVRMTALVVAATEQARAGAERVLGIL"
     assembly_gap    6423..6537
                     /estimated_length=115
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6759..6797
                     /estimated_length=39
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6798..>7681)
                     /locus_tag="PLV68_03100"
     CDS             complement(<6798..>7681)
                     /locus_tag="PLV68_03100"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HRE00257.1"
                     /translation="GAEVYLRPEPAQGMFLNFANVLQTSRRKPPFGIAQVGKSFRNEI
                     TPGNFIFRTREFEQMELEFFVPPAEGPKWYEYWCNERLNWYIELGIPADMLMMRPHDA
                     DELSHYSTGTADVEFKFPWGWGELEGVAQRTDYDLKQHAQHSGQKLEYFDQASGERYV
                     PYVIEPAAGATRTMAAFLLAAYDEDVVNGETRTVLRLHPRLAPYKVAVLPLSKKESLT
                     PLAKQLYRDLSAHYMVEYDETQAIGRRYRRQDEIGTPLCVTVDFDSLEDGAVTVRDRD
                     TTEQVRVPIDELVATVRT"
BASE COUNT         1230 a         2639 c         2522 g         1107 t
ORIGIN      
        1 cctcgcgcaa cacccacgcc agcgcatgga cgctttccag cgcggcgatg atgccttcgg
       61 tccgagccag cacctggaag gcctcgatca cctcggcgtc ggtgacgctc ggatactcgg
      121 cccggccgat cgaggcgagg tacgaatgct cggggccgac gccggggtag tcgagcccgg
      181 ccgagatcga ctcggcttcc agcacctggc cgtactcgtc ttgcatcagg tagctacgca
      241 tgccgtggac cacgcccggc acgccgcgag caaccgccgc gccgcccgct ggttcgacac
      301 cgatcaggcg cgagcgggtg tcgacgaagc cgctgaagat gccaatcgcg ttcgatccgc
      361 cgccgacgca ggcgacgacc gcgtcgggga cgtcgccggt caatgcgacg cactgctcgt
      421 gcgcctcgct gccgaccacc cggtggaact cgcgcaccat ccagggatag gggtgcggcc
      481 ccatcaccga gccgaggcag tagtggctct cgccgacggt ggcgacccag tcgcgcatcg
      541 cctcgttgac cgcgtccttg agtgtgcggc tgccactgtt gaccgcttcg acctcggcgc
      601 cgagcaggcg cattcggaac acgttgagcg cctggcgttc gacgtcgacg gctcccatgt
      661 acaccttgca actcaggccc atcaacgccg ccgcggtcgc gctggcgaca ccgtgctgac
      721 cggccccggt ctcggcgacg agacgggtct tgcccatccg cttggccaac agcgcctggc
      781 cgagcacgtt gttgatcttg tgcgaaccgg tgtggttgag gtcttctcgc ttgagcagca
      841 gacggatccc caacgcacgc cccaggttgt ggcactcggt gaggatgctc ggccgcccgg
      901 cgtagtcgcg aagcagtccg tcgagctcac cgcggaagcc ggggtcgttc cacgcctcgc
      961 ggaacgccgc ctccagttcc atgcacgcag gcaccagcgt ctcgggaacg taacgtccgc
     1021 cgaactcgcc gaaccgaccg gtgtcggagg gttcggacat gatcgacgcg ccggtcggag
     1081 ggtgcgatgg gtggatggtc attggcggtg gctctctcag cggctggctt cgtcgtaact
     1141 gttcgggacg gtcattcgtc gtcggcccag tcgtagggca ggttgtcggg tccgatgtag
     1201 ggcgtgggcg ctgcggcccg ggcccgctcg atgaacgcct tgaccttgag cgcgtccttc
     1261 ttgcccggtg aacgctcgac accactggac acgtcgacgc cccaggggcg cacctgggcg
     1321 accgccgccg cgacgttgtc ggggtcgagg ccaccggcca ggatcagccg cacggcgccg
     1381 gacacttcgt cggccaacga ccagtcgaac atccgccccg accccggcgt ggaaccgtcg
     1441 accagcacca gatcggtgcc gaactggtcg gcccggcggg catcggtgct gccggccgcg
     1501 accgccttga tcacccagcg cacgtggcgg gcgacctcgg caacgtcgct ggggctttcg
     1561 tgaccgtgca gttgggccgc cttgaccccg gacttgtgga ccatgtcgat cacccgcgac
     1621 gggtggtcgt cgcggaacac cccgacggtc aggatctccg gcggcaagcg gcgggtgatg
     1681 tcgtagacct gctgggctgc gacctggcgg gtggacggcg cgaacacgaa ccccaccgca
     1741 tccgcgccca tcgccacggc gaacagcgcg tcgtcctcat tggtgattcc acagatcttg
     1801 acgaacatcc ggcacgaacc gtaccccgcg cgtcatcggg aacaccggat cgattcacgg
     1861 catcacgtca cgcgacgccg cgcaacgcac cgatcgtggc ggccggatcg gcagcggtca
     1921 ccagggtctc ccccaccagc acggcgtgat atcccgccgc ctgcaggctg cgggcgtcgt
     1981 cggcaccgcg gacgccgctc tcggcgacct tgacgacatc gtcggggatc accgccgcca
     2041 tccgtaccgc tcggtcgtgg tcgacctgga agctcaccag gtcgcgctgg ttgacgccga
     2101 cgatcgtggc gcccgacgcg agcgccacgt cgagctcggg ttcgtcgtgg atctcgacca
     2161 acacgtcgat gccgatctcg tcggcgatgc ggtgcagtgc gaccagctcg tcccgtccga
     2221 gcgcggccgc gatcagcaac acgcaatcgg cgcccatcaa gcgggcgtcg cacacgtcgc
     2281 gggcgctgac cgtgaagtcc ttgcgcagca cgggcagcgt taccgcagcg cgcgccgcct
     2341 gcaggtcggc caccgaccca ccgaagaact ccacgtcggt cagcaccgac atcgcggcag
     2401 caccaccagc ggcgtagcgc tgggccatct cggccgggtc gaggccgacg ttgagcgcac
     2461 ccttggacgg cgagcgtcgt ttgatctcgc tgatcacggc caactcgccg tgctcgctgg
     2521 cacgccggag cgctgtgccg aacggccgga tcggtgccgc cgacgcagca cgctcgatca
     2581 gcgcgcccag cgggcggtcg tcggtcgcag ccacctcgcg gtggcgcgcg acgatgcgat
     2641 cgagataggt ggccatcagc tcccgggcgt gcccttgcct gcgaccggct cgaggaagat
     2701 cagctcggga cgccctgcgg cgaactcacg cgaggccatg cccatcgcct tgatgccgtc
     2761 ggtcgaaccg tggtgcacga acgactcctg gctgtcgtag aactcgtaga accacaccag
     2821 gtcggggtcg ttcgcgtcct tgtgcatgat gtactgcagc gtgccgggct cgctctgcac
     2881 gttctggatg ccgacctgca gtgcttcgat gagctgatcg cgcatgccgg gcttggcggg
     2941 gatcttggcg atgatggcga cattggccat gggaggctcc gttctcggtc gtgccggtac
     3001 gtcggtgtcc ggctgtgggt cagaccgtac tcaagccgtc gtgcaccgcc cacatctggg
     3061 cgtagcggcc accctcggcg agcaggtcgt cgtgtgaacc ccgctcgagc acccgtccgg
     3121 cgtcgatgac cacgatctcg tcggcggcgc gggccgtggc caagcgatga gcgaccagca
     3181 ccgtcgtgcg gttggcgctg accgcatcca tcgccgaccg caccgtggcc tccgcggcca
     3241 ggtcgaggtt ggccgtggcc tcgtcgagca gcaggatcac cggatcgacc agttgcgccc
     3301 gggccaacgc gatcagctga cgctgccccg acgacaacga ccggccccgt tcggacacgg
     3361 tggtgaggta gccgtcggcc agcccggcga tgaacccgtg ggcgccgacc gcgcgagcgc
     3421 tctgctcgac ctcggcgtcg gaggcgtcgg gacggccgta ggcgatgttg tcgcggatcg
     3481 tcccggtgaa caggaacggt tcttgaggaa cgacaccgag ttggcggcga tacgcgctga
     3541 ggtcgaggtc acgcagatcg ataccgtcga tgcggaccat gcccgcggtc gggtcgtgga
     3601 accgagccag cagcttgacc acggtcgact tacccgcgcc ggtctcgccc accagcgcca
     3661 cggtgcgacc aggagcgatg tgcagatcga ccccgctcaa cgcttcgttg cgcgcgccgg
     3721 gatagacgag atgcacgtcg accagttcga tctcgccgcg caaccggccc ggatcgatgg
     3781 gctgagcggg cgggggtgtc gcggtcggcg tctgcaggag ctcggtgatc ttggcggtcg
     3841 acgcgctggc ttgctgccaa ctgtcgaaca cctgcgagag ctgctggatc ggggcgaaga
     3901 acacgttcaa cgacagcacg aacgcgatga ccacaccggg ggtcgtgatg ccgtcggtga
     3961 cgaaggctgc gcccgcaccg agcaccgcga ccgcggccag atcggccaac agccccacgc
     4021 cgggaaagta cagggcaagc agcttctggg cgcgcagcct ggccgcgagg taggtgccgc
     4081 tcgtggcccg gaagcgatcc gcgttgaccc gctcacgacc cgacgcttgt gtcagccgca
     4141 cacccgacag gttctcctgc agatccgcgt tgacgttcgc aattgcgtca cgcgcgctgc
     4201 ggtaggccgc gctggagtgc cgctggtacc accacgtcgc ccccgccaga ggcaccagcg
     4261 cgctcgctgc caccagcgcc agcggccacg actggatcgc cagatagacc gccacaccaa
     4321 gacaggtcgc cataccgacc accgcgttga ccagcccggt ctgcaccaac tgcgacagcg
     4381 cttcgacatc ggtggtcatc cgggtcatca cccgcccgtc catctcccgg tcgtagtagt
     4441 cgagcgacaa ccgctggagg tggccgaaga tgcgcatccg caggctgagc agcatccgtt
     4501 ccgccgtccg accggtgacg acggcaccga cccaggtgac gcaccaatcg ctcagcccga
     4561 cgatgacgaa caccagcgcc gcgccgcgca agaccgcacc gtcgccggcc gagatgccgt
     4621 ggtcgatgcc gaagcgcatg atcgccggac cgagcacggt cagcaccgca tcggtcgcga
     4681 ccagcgccaa gccgatggcc agccaccgtc gccagcgacg ggcgaagcgg aggatgcgga
     4741 aagcgcccgc gccgttccca gccgtgccgt tggcgagttc ggcggaggct tcaccgatca
     4801 cgtcgatgcc cggttcgtca tcggccgccg gcaagcggcc gagcgcagcc agcagatcgg
     4861 aggttggggc caggttcgca ccgaagttgc cgccgcccgg tggaccgatc acggtgcgtg
     4921 tcgtgatcgc gctggctacc gccgccggac gagcccgcac cggccaggcc gacgccgtga
     4981 tcccgacatc gtcgagcccg gcatcgtcac gctcgacgtc gcccggatcg ctggtcgtcg
     5041 ctgtggccac ggcagcgcta ccgaggccgt cgccgagcag gtcgcgatag cgggcgctgc
     5101 gggccagcag ctcgtcgtga gtgcccgtgt cgagcacccg gccgttctcg atcaccacca
     5161 cccggtcggc caggcgcacg gtcgaggccc gatgggcgac gagcaacgtc gtacgaccag
     5221 ccatcacatc gtggagcgca gcgtggatgg cctcttcggt cgccgagtcg accgccgagg
     5281 tcgcgtcgtc caataccagg atgcgcgggt tggtgacgat cgcccgcgcg agcgcgatgc
     5341 gctggcgttg cccgcccgac agcgtcatgc cgcgctcgcc gacgacggtg gcatacccat
     5401 cgggcaacgc ttcgacgaac ccggccgcgc ccgcgatacg ggccgcctcg cgcacctcgg
     5461 cgtcggtcgc gtcgggctga ccgtaggcaa tgttggcgtg gatcgagtcg ctgaacagga
     5521 acgactcctc gaaggcaacc ccgacctggc gccgcagcga ggccagggtg gcgtcgcgca
     5581 cgtcggtgcc gtcgatgcgg atcgacccgg agtgcacgtc gtagaagcgg ggcaacagcg
     5641 cggtcagcgt gctcttgccc gagccgctcc cgccgacgag cgcgaccact tctccggcct
     5701 ccacccgcaa cgagagcccg tcgaggatcg gctccgtggt ggtgtagccg aagctgacgt
     5761 catcgaagtc gatcgcgccc ggcccgtcag ggaggggctg nnnnnnnnnn nnnnnnnnnn
     5821 nnnnnnnnng aggatgccca atacccgctc ggcaccagcc cgcgcctgtt cggtcgcggc
     5881 cacgaccaac gctgtcatcc gcaccggtgc ggtcatctgc accagatacg tggcaaaggc
     5941 caacagcgtg ccgaccgaca ggcggtcgtg cagcgcaagc caaccgccca caccgagcac
     6001 cgcggcctgg gccagcatcg ggatcgcagc cagggtcgag gtcgcccggg cctgcaaccg
     6061 caccaaacgg acccgggccc ggtacagcgc ggtggccgtg tcggccagtc gggcgatctc
     6121 gcggtcctcc gccccgaagc ccttgacgat gcgcacacca gacaccgcct cgtcgaccac
     6181 accggccact tcggcggcgc gctgctgcgc gtcccacgtc gcgggaaaca ccgtggcccg
     6241 caagcgcagc gcggtgacga gcatcagtgg cagagcagcc accatcacca ggctgagcag
     6301 cggcgacaac cacaccatcg cgacgatgga gatcacgagc atgatcaggt tgccgagcat
     6361 caacggcaag aaggcgagca actgctggat caggcccaga tcgccggagg cccgcgacac
     6421 cannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnccg
     6541 tcgggcgacc gcgagcacga acgtgatcag gccgagcagc gccaacaacg cgagccacgg
     6601 cgcgaccgag gttcgggacg cgacgatgcc gtcgtccacc acgattcgct gcacgatcgg
     6661 cgcgacgccg agcgcaccct ggccgacgat cgaggccgtg acggccaaca ccacgtcgcg
     6721 acgatgcgcc agcagaaacg gcgagagccg cctgatccnn nnnnnnnnnn nnnnnnnnnn
     6781 nnnnnnnnnn nnnnnnnggt gcgcaccgtg gcgaccaact cgtcgatcgg gacccgcacc
     6841 tgctcggtcg tgtcgcgatc gcgcacggtg accgcgccgt cctccaacga gtcgaagtcg
     6901 accgtgacgc acagcggcgt accgatctcg tcctgacggc ggtagcggcg gccgatcgcc
     6961 tgcgtctcgt cgtactcgac catgtagtgc gcgctgaggt cgcggtagag ctgcttggcc
     7021 agcggcgtca gcgactcctt cttcgacaac ggcagcacgg ccaccttgta cggcgcgagc
     7081 cgaggatgca ggcgcaacac ggtgcgggtc tcgccgttga cgacgtcttc gtcgtaggcg
     7141 gcgagcagga aggccgccat ggtacgggtg gctccggcgg ccggctcgat cacgtacggc
     7201 acatagcgtt cgccactcgc ctgatcgaag tactccaact tctggccact gtgctgggcg
     7261 tgctgcttca ggtcgtagtc ggtgcgctgg gcaacgcctt ccaactcgcc ccatccccac
     7321 gggaacttga actcgacgtc ggcggtgcct gtcgaatagt ggctcagctc gtcggcgtcg
     7381 tgcggacgca tcatcagcat gtcggccggt atgcccagct cgatgtacca gttgagccgc
     7441 tcgttgcacc agtactcgta ccacttcggc ccttcggcgg gcggcacgaa gaactcgagc
     7501 tccatctgtt cgaactcacg ggtgcggaag atgaagttgc cgggtgtgat ctcgttgcgg
     7561 aagctcttac ccacctgcgc gatgccgaac gggggcttgc ggcggctggt ctgcaacacg
     7621 ttggcgaagt tgaggaacat gccttgggcg ggctcgggac gcagatacac ctcggcaccc
     7681 g
//
LOCUS       DAORNF010000048         7649 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_7080_length_7649_cov_1.710034, whole genome shotgun sequence.
ACCESSION   DAORNF010000048 DAORNF010000000
VERSION     DAORNF010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 7649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7649
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7080_length_7649_cov_1.710034"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="PLV68_03105"
     CDS             <1..409
                     /locus_tag="PLV68_03105"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRE00258.1"
                     /translation="NFVPTHVCESAAELAAELGLPGEELTRTIEAYNAGARHGTDPEF
                     HKASHWVRPLEGALGATSCGSLRTFTLGGLVTDVDSRVLRDDGTPVRRLYAAGRVASG
                     LPAWGYVSGSSLGDCTYFGRRAGRSAAGVLAAR"
     gene            complement(406..1356)
                     /locus_tag="PLV68_03110"
     CDS             complement(406..1356)
                     /locus_tag="PLV68_03110"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRE00259.1"
                     /translation="MEHTRRVLVTGATGKLGRPLVAALAAEHDVWAVARFRDRAIEAE
                     LTAAGVTCVRGDLAGDLSALPDDPDAFDSIWNLAVAKSGDWGADLDANLGGVIALLHR
                     YPNLGSFVHISSCEVYDPDADRPYREDSPRGNARHNTDVAAMRTYSVAKIAAESAVTA
                     TAMALGVPAVIVRLSAPYGDTWGWPVAHLAKIRAGEAISVHPDRPNTFSLLHVDDIIA
                     MMPSLSRAAAVPAQVVNLAGSEAVSIEEWVGELGRLVGVDPILHDSPHALRSIPVDVT
                     RMQALIGTGSIDWRTGMGRIASAAGADGAATGSPPLPSGT"
     gene            1607..2224
                     /locus_tag="PLV68_03115"
     CDS             1607..2224
                     /locus_tag="PLV68_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00260.1"
                     /translation="MTTSSGANHIIISSDTHAGADLREYKPYLASKWHDEFDAWADAY
                     SSPWDDLVNDTADRNWDSDLRLQQTQADGVVAEVLFPNTIPPFFPSISNIVPLPKDDT
                     DYQRRWAGIQAHNRWLVDFCSKAPDQRRGLVQVFPHKVDDAVAEIKWAGWSSLPRRPT
                     IRWSSRCSPTRTSRCGKRSRNPIWPSVRTPAVVSPSTRTIRPAPR"
     gene            2128..2814
                     /locus_tag="PLV68_03120"
     CDS             2128..2814
                     /locus_tag="PLV68_03120"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRE00261.1"
                     /translation="MWEAIEESNLAFGTHTGSGEPEYPNHPASSAVMMFEFGLFTNRT
                     LSHLIIGGVFERFPELRFTMTEQGVKWVAETLSRLDGTYTAVADRPWLEMFVREAYKD
                     LSLTPTEYFQRNCFIGASVLRPGEVATAANLGTSNVMWGSDYPHRESTYPYSVHSLRV
                     ALADLNEQQRREVLSETAAKVYGFDLDALRPLADKYGPSPEALSTPLTADELPTDNEW
                     FLPEMLTLAR"
     assembly_gap    2862..2928
                     /estimated_length=67
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2929..>3926)
                     /locus_tag="PLV68_03125"
     CDS             complement(<2929..>3926)
                     /locus_tag="PLV68_03125"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRE00262.1"
                     /translation="TSGTTGRAKGVVWTQDNYQWAGRVGARHLQLRAEDRILLYVPLF
                     HANAQSYVLMPALWVGATIIVMPRFSASRFWPVSVEERATVASMIPFAVKALRAHDVP
                     ADHVYRCWGNGVIVPGWDKRFSVSTVSWWGMTETVSQPIVSTPGEAGHPLAMGRCAPE
                     YEVRIVADDGTVLTPPAVGLLQVLGERGLSLFAEYLDDPQATASTFTADGWLETGDRV
                     EAHDDGWFSFVERDKDMLKVGAENVAALEIERVVNTVTGVRAVAVVAAPDPMLEEIPV
                     AFVVPTDDAPADLGDQIVQHCRSQLADFKVPRLVSIVDALPESLLGKIAKNVLRKR"
     assembly_gap    3927..4118
                     /estimated_length=192
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4119..4472)
                     /locus_tag="PLV68_03130"
     CDS             complement(<4119..4472)
                     /locus_tag="PLV68_03130"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HRE00263.1"
                     /translation="MGLFEPFADPNLASLFRRQVAARADHPFVVWRDLDGHRAEWSYA
                     EAARIVDGLAAGLWDLGVRAGDRVGVHAGNCPEFIFTVFAIAELGAVMVATNVRSSID
                     ELTDYLARAQCSVVFT"
     assembly_gap    4515..4633
                     /estimated_length=119
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4634..5414
                     /locus_tag="PLV68_03135"
     CDS             <4634..5414
                     /locus_tag="PLV68_03135"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRE00264.1"
                     /translation="RLLQDRVVVITGAGPGTGAALTHTAAREGAAVVVAARDVSRVSA
                     IAAEVVEAGGRAVALPVDVTDTASCQALAQGALEAFGRIDALVNAAFYTEPEQSLWDL
                     EESTLQRTLDVNITGTWRVMKAVVPHLTRPGGAIVAIGSQAGTKSSATLGPYAAAKAA
                     VHSLTCSAAVQLGPEGVRVNGVLPGSIDGPGLLEFAEERGKRLGTSTEAELRLRRGRS
                     PLGRIVTPQEIAEATVFLCSDRASGITGTLVHLDCGQHLNV"
     gene            complement(5416..5811)
                     /locus_tag="PLV68_03140"
     CDS             complement(5416..5811)
                     /locus_tag="PLV68_03140"
                     /inference="COORDINATES: protein motif:HMM:NF024092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HRE00265.1"
                     /translation="MNVTEPTSDSVRDRIVAACDAYVAALDSGDVESAVACFAPDATQ
                     EEPAGSLPNVGTDAIRAFFESRRSRIRVRRCSPVTVVGYRAVFQIHVVVDDGAVELVS
                     SDVVELDDQFRFRSIVAFPDRHATLFDRA"
     assembly_gap    5952..6036
                     /estimated_length=85
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6037..6391)
                     /locus_tag="PLV68_03145"
     CDS             complement(<6037..6391)
                     /locus_tag="PLV68_03145"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRE00266.1"
                     /translation="MEQQSKLDRRERLLDAAAELLDSGGYDALTARAIAARGNVSVGL
                     LYHYFGDKHDVFGALLQREQATLTALLDGAPRESLPELLRHVVPATTEQWRRIGRMTA
                     AWAADRPPAPPETMSA"
     gene            6492..>7114
                     /locus_tag="PLV68_03150"
     CDS             6492..>7114
                     /locus_tag="PLV68_03150"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRE00267.1"
                     /translation="MSVSFDYSGCRVLVTGGSSGLGLAVARGFAHAGAQVTITGRADR
                     PDSYADDLSAFTYVSCELADAESVEALAARVPAVDVLVNNAGANLKTEDEWRPDVFER
                     SVALNLFGPFRLSSACLDALARSSWDGGASVINVASMAAYFAVDPVPAYGVAKAGVVQ
                     MTKSMASAWAARGVRVNAVAPGLTRTRMTAVTVSQPERTEATLARTPL"
     assembly_gap    7115..7257
                     /estimated_length=143
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7258..>7649
                     /locus_tag="PLV68_03155"
     CDS             <7258..>7649
                     /locus_tag="PLV68_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00268.1"
                     /translation="EHVLRIDDLGAPVLSEHQQQLLADAEAVPVTLSVDTVLAAARDR
                     SGLSDYGSMDFLDRLSLWLGEIDQDPNRTAYIRKRLFDDCVRFATSRLRLTDLLRRHP
                     EIHDVQIVRPVIVVGMPRTGTTHLVNVL"
BASE COUNT         1161 a         2425 c         2348 g         1109 t
ORIGIN      
        1 gaacttcgtc cctactcacg tgtgcgagtc cgccgccgag ctggccgctg agctcggact
       61 gcctggcgag gaactgaccc gaacgatcga ggcctacaac gccggggcgc gacacgggac
      121 cgaccccgag ttccacaagg ccagccactg ggtgcggccc ctcgagggcg cgctcggcgc
      181 aacctcgtgt gggtcgctgc ggaccttcac cctcggcggc ctcgtcaccg acgtcgacag
      241 ccgcgttctg cgcgacgacg gcacaccggt tcgccggttg tacgccgccg ggcgcgtcgc
      301 gtccggcctg ccagcgtggg gttatgtcag cgggtcgtcg ctcggcgact gcacctattt
      361 cggccgccgt gcaggcaggt ctgcagccgg agtgctggcc gctcgttaag tgccgctggg
      421 cagcggcggc gatcccgtcg cagcaccgtc cgcgccggcc gccgacgcga tgcggcccat
      481 gccggtccgc cagtcgatcg agccggttcc gatcagggcc tgcatgcgcg tcacgtccac
      541 ggggatcgag cgcaacgcgt gcggcgagtc gtgcagaatc ggatcgacac cgacgaggcg
      601 gccgagttcg cccacccact cctcgatcga gacggcctcg ctcccggcca ggttgacgac
      661 ctgggctgga acggccgcag ctctcgacag cgacggcatc atcgcgatga tgtcgtcgac
      721 atggagcagg gagaacgtgt tgggccggtc gggatggacc gagatcgcct cgccggcacg
      781 gatcttggcc aagtgcgcga ccggccaacc ccacgtgtcc ccgtacggag cgctgaggcg
      841 gacgatcacc gccgggacac ccagcgccat ggccgtcgcc gtcaccgctg actcggctgc
      901 gatcttggcc acgctgtagg tgcgcatcgc ggcgacgtcg gtgttgtggc gagcgttgcc
      961 gcgcggtgaa tcctcgcggt acgggcggtc ggcgtcgggg tcgtacacct cgcacgacga
     1021 gatgtgcacg aacgagccga ggttcggata gcggtgcagc agcgcgatca ccccgccgag
     1081 attggcatcg agatcggccc cccagtcgcc cgacttggcg accgccagat tccagatcga
     1141 gtcgaacgcg tccgggtcat cggggagggc cgacaggtct cccgcgagat ccccgcgcac
     1201 gcaggtcacc cccgctgcgg tcagctcggc ttcgatggcg cgatcgcgaa agcgggccac
     1261 ggcccacacg tcgtgctctg cggcgagtgc ggcgaccagc ggacgcccga gcttgccggt
     1321 cgccccggtg accaggaccc gtcgtgtgtg ctccatgcga gtgactgtaa cgacgctccg
     1381 aatcgcgtcg gcctgggcat cccagtggtc ggcatcgctg gtccgcccgg catgcacggc
     1441 gacgtttcac ctgtctcagt agatggcaag ctggccttca acgatcgcgc gcggcgactt
     1501 atcgtgcaca cacgagacgg ttctcttcga gccaccgaac gcgccgtagg cggccgaggt
     1561 gactcaccgt aagcgagaac gtcagttcat cgacggaagg aatcccatga ccacgagctc
     1621 tggtgccaac cacatcatca tctcgtcgga cacccacgcc ggggccgact tgcgtgagta
     1681 caagccatat ctcgcgagca agtggcatga cgagttcgac gcctgggccg acgcctactc
     1741 cagcccgtgg gacgacttgg tcaacgacac cgcagaccgg aactgggaca gcgacctgcg
     1801 tctgcaacag acccaggccg acggcgtggt ggccgaggtg ttgttcccca acaccatccc
     1861 gccgttcttc ccgagcatct ccaacatcgt gcccctcccc aaggacgaca cggactatca
     1921 gcgtcggtgg gcaggaatcc aggcccacaa tcgttggctc gtcgacttct gcagcaaggc
     1981 ccccgatcag cgtcgtggcc tcgtccaggt ctttccgcac aaggtcgacg atgccgtcgc
     2041 cgagatcaag tgggcggggt ggtcatccct gccgcgccgc ccaaccatcc gctggtcgag
     2101 ccgctgttca ccgacacgta cctcccggtg tgggaagcga tcgaggaatc caatctggcc
     2161 ttcggtacgc acaccggcag tggtgagccc gagtacccga accatccggc cagctccgcg
     2221 gtgatgatgt tcgagttcgg tctgttcacc aaccgcaccc tgtcgcacct gatcatcggt
     2281 ggcgtgttcg aacggttccc cgagcttcgc ttcacgatga ccgaacaggg tgtcaagtgg
     2341 gttgccgaga cactgagccg cctcgacggc acctacaccg ccgttgccga ccggccgtgg
     2401 ctggagatgt tcgtgcgcga ggcctacaag gatctctccc tgaccccgac ggagtacttc
     2461 cagcggaact gcttcatcgg cgccagcgtg ctgcgcccag gtgaggtcgc gacggccgcc
     2521 aatctcggta cgtccaacgt gatgtggggt tcggactacc cccaccgcga gtccacctat
     2581 ccgtattcgg tccactcgct gcgggtcgcg ttggccgacc tgaacgagca gcagcgacgc
     2641 gaggtgctca gcgagacggc cgccaaggtg tacgggttcg acctcgacgc gttgcgcccg
     2701 ttggccgaca agtacggccc gagcccggaa gcgctgtcga ccccgctcac cgcggacgaa
     2761 ctgcccaccg acaacgagtg gttcctgccc gagatgctca ccctcgcccg ttgagccacg
     2821 cgcgtggtca ccggccttcg gccggagacc cgcgacgtgg gnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncc cgcttgcgta
     2941 acacgttctt ggcgatcttg ccgagcaagc tctccggcag cgcatcgacg atcgagacga
     3001 gtcgcgggac cttgaagtcc gcgagctgag atcggcagtg ctgcacgatc tggtcgccaa
     3061 ggtcggcggg agcgtcgtcg gtggggacga cgaatgcgac ggggatctcc tccaacatcg
     3121 ggtcgggggc ggccaccacg gccaccgcgc ggacacccgt cacggtgttg acgacgcgct
     3181 cgatctccag cgcggcgacg ttctcggcac cgactttgag catgtccttg tctcgttcga
     3241 cgaaggagaa ccatccgtcg tcgtgagcct cgacgcggtc gccggtctcg agccagccgt
     3301 cggcggtgaa cgtcgatgcc gttgcctgcg ggtcgtcgag gtactccgcg aacaacgaca
     3361 agccgcgttc gccgagcacc tggagcaggc caacggcagg aggtgtgagg acggtgccgt
     3421 cgtcggcgac gatccggacc tcgtactcgg gcgcacagcg gcccatcgcg agcgggtgac
     3481 cggcctcgcc gggtgtcgac acgatcggct gtgacaccgt ctcggtcatt ccccaccagc
     3541 tcacggttga caccgagaag cgcttgtccc aaccgggcac gatcacgccg ttgccccagc
     3601 accgatacac gtggtctgca ggcacgtcgt gggcgcgcag cgccttgacc gcgaacggga
     3661 tcatcgacgc caccgtggcg cgctcctcga cagacacggg ccagaaccga ctggccgaaa
     3721 agcgtggcat cacgatgatg gtggcaccga cccacaacgc cggcatcaac acgtagctct
     3781 gcgcgttggc gtggaacagg gggacgtaga gcaggatccg atcctccgcg cggagttgca
     3841 gatggcgagc tccgacccgg cccgcccact ggtagttgtc ctgagtccag acgacacctt
     3901 tggcgcggcc tgtggtgccc gatgtgnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngg tgaacaccac cgagcactgg
     4141 gcgcgtgcca ggtaatcggt gagctcgtcg atcgaactgc ggacgttggt cgcgaccatc
     4201 accgcgccga gttcggcgat cgcgaacacg gtgaagatga actccgggca gttgccggcg
     4261 tggaccccga cccggtcgcc ggcgcggaca ccgaggtccc acaagccggc ggcgagaccg
     4321 tcgacgatgc gggccgcctc cgcgtacgac cattcggccc gatggccgtc gaggtcgcgc
     4381 cacacgacga acgggtggtc ggcgcgtgct gcaacctggc gccggaacag gctcgccagg
     4441 ttcggatcgg caaaaggttc gaagagtccc atcgtcgtcg aaacatacga ggtagccgtc
     4501 cgagcgcggg tgacnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4621 nnnnnnnnnn nnntcggctt cttcaggatc gcgttgtcgt gatcaccggc gcaggcccgg
     4681 gcacgggcgc cgccctgacg cacacggcag cccgcgaagg agccgccgtc gtggtcgcgg
     4741 cgcgagacgt gagccgagtg tcggcgatcg ccgccgaggt ggtcgaagcg ggaggccgag
     4801 ccgtggcgct ccccgtcgac gtcaccgata ccgcatcgtg tcaggcgctg gcgcagggcg
     4861 cgctcgaggc gttcggtcgc atcgacgcac tcgtcaacgc cgccttctac accgagccgg
     4921 agcaaagtct gtgggatctc gaggagtcca cgctgcaacg gactctcgac gtcaacatca
     4981 ccggcacgtg gcgggtgatg aaggcggtcg tcccccacct gacacggccc ggtggcgcca
     5041 ttgtcgcgat cggttcccag gccggaacga agtcgtcggc cacgttgggg ccctatgccg
     5101 cagcaaaggc tgccgtgcac tccctgacct gttcggctgc tgtgcagctc gggccggagg
     5161 gcgtgcgggt caacggcgtg ttgcccggct cgatcgacgg ccccggcttg ttggagttcg
     5221 ccgaagagcg cggcaagcgc ctcggtacgt cgacggaggc cgaactgcgt ttgcgtcgcg
     5281 ggcgctcacc cctcggccgg atcgtgactc cccaggagat cgcggaggcg accgtgttct
     5341 tgtgctccga tcgcgcgagc gggatcaccg gaacgctcgt ccacctcgac tgcggccaac
     5401 acctgaacgt ctgagtcagg cccgatcgaa cagcgtcgcg tggcggtccg ggaaggcgac
     5461 gatcgatcgg aaccggaact ggtcatccag ttcgaccaca tcgctcgaca ccaactcgac
     5521 cgccccgtcg tcgaccacga cgtggatctg gaacaccgcg cggtacccga cgacggtcac
     5581 cgggctgcag cgtcggacgc gaatccgcga ccggcgtgac tcgaagaacg cgcggatggc
     5641 gtcggtgccg acgttgggca ggctgcccgc tggttcttcc tgggtggcat ccggagcgaa
     5701 acacgcgacg gccgattcga cgtcaccgct gtcgagcgcg gccacgtagg cgtcacacgc
     5761 agcgacgatc cggtcgcgga cggaatcgct cgttggttcc gtgacgttca cgccgatacc
     5821 gcccgcacga tggactcgat cgcgtagtcg acgagatcgt cggcggaggc gtcacgggtc
     5881 caaccgttga ccagatcgtc ggcgaggccc atcaacgcgg accacaccca ggcgaccatc
     5941 gcgggaccgt cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngagc cgacatggtt tcggggggag
     6061 cgggcggacg atcagccgcc caggccgcgg tcatccgacc gatccggcgc cattgctcgg
     6121 tggtcgcggg cacgacgtgg cgcagcagtt ccgggagcga ctcgcggggc gcgccgtcga
     6181 gcagggcggt gagcgtcgct tgctcgcgct gcaacaatgc cccgaacacg tcgtgcttgt
     6241 cgccgaagta gtggtagagg agcccgacgc tgacgttgcc cctcgccgcg atcgcgcgtg
     6301 ccgtcagcgc gtcgtagccg cccgagtcga gcagttcggc cgccgcatcg agcagtcgct
     6361 cgcgccgatc gagtttggac tgttgctcca tcccactcac tggaacacga tcctagcaga
     6421 atctttgaac atcgttcaat cttggtaccg gacagggcga ccgaggccca ccggaaggag
     6481 agcactgcat cgtgagcgtg agtttcgact acagcggatg ccgcgtgctc gtcaccggtg
     6541 gttcgagcgg tctgggtctc gccgtcgccc gcgggttcgc gcacgcgggt gcccaggtga
     6601 ccatcaccgg ccgcgccgac cgtcccgaca gctacgccga cgatctgtcc gccttcacgt
     6661 acgtgtcgtg tgaactcgcc gacgccgagt cggtggaggc gctggccgcc cgggtcccgg
     6721 cggtcgacgt gctcgtcaac aacgccggtg ccaatctcaa gaccgaggac gagtggagac
     6781 ccgacgtgtt cgaacgctcg gtcgcgctca acctgttcgg ccccttccgg ctcagttcgg
     6841 cgtgcctcga cgcgctggcg cgcagctcct gggatggcgg tgccagcgtc atcaacgtgg
     6901 cgtcgatggc cgcctacttc gcggtcgatc ccgtcccggc ctacggggtg gccaaggccg
     6961 gcgtcgtgca gatgacgaag tccatggcgt cggcgtgggc cgcacgcgga gttcgggtca
     7021 acgcggttgc cccgggactg acccggaccc ggatgaccgc ggtcaccgtc tctcagcctg
     7081 aacgcaccga agcgacactc gcccggacgc cgctnnnnnn nnnnnnnnnn nnnnnnnnnn
     7141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncag
     7261 aacacgtcct ccgcatcgac gacctcggtg cccccgtcct gtcggagcac cagcagcagt
     7321 tgctggccga cgccgaagcg gtgccggtga cgctctcggt cgacacagtg ctcgccgcag
     7381 cacgagaccg gtccggactc tcggactacg gatcgatgga cttcctcgat cggctctcgc
     7441 tgtggctcgg cgagatcgac caggacccca accgaaccgc ctacatccgc aagcgtctct
     7501 tcgacgactg cgtgcggttc gcgacgagcc ggctgcggct caccgatctg ctgcgccgtc
     7561 accccgagat ccacgacgtc cagatcgtcc ggcccgtgat cgtggtgggg atgccgcgca
     7621 ccggcaccac gcatctcgtc aacgtgctc
//
LOCUS       DAORNF010000049         7550 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_7199_length_7550_cov_1.840961, whole genome shotgun sequence.
ACCESSION   DAORNF010000049 DAORNF010000000
VERSION     DAORNF010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 7550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7550
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7199_length_7550_cov_1.840961"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="PLV68_03160"
     CDS             complement(<1..259)
                     /locus_tag="PLV68_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HRE00269.1"
                     /translation="MTKADGLAAPVTPDPLRTRFCTLTGVQVPIVQTGMGWVSGARLT
                     AATANAGALGIIASATMDLDQLAHAIDEVRQRAPGKPFGVNL"
     assembly_gap    397..406
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<407..1059)
                     /locus_tag="PLV68_03165"
     CDS             complement(<407..1059)
                     /locus_tag="PLV68_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HRE00270.1"
                     /translation="MEGTRGMTVTGTEPKPAESASAHLADIVVIAAAEAFRGDGEIFA
                     SGMGTIQMLGARTAKATFEPDLMVSDGEAFFVGNDLPIGSTDKVVEAWVPFRTVFDML
                     WSGRRHVMMGASQIDRYGNTNIANIGPFQQPKVQLLGVRGGPGNTISHATSFFIPRHS
                     TRIFVDHVDMVSGLGYDRVARLSPVAQGHHRLPRVITNLAVLDFETPDHSMRLRSVHP
                     "
     assembly_gap    1124..1210
                     /estimated_length=87
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1211..>1441)
                     /locus_tag="PLV68_03170"
     CDS             complement(<1211..>1441)
                     /locus_tag="PLV68_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA--acetate/3-ketoacid CoA transferase
                     subunit alpha"
                     /protein_id="HRE00271.1"
                     /translation="LDAAFVHMNRADARGNAQFLGPDLYFDDLFLGACEPGHRFVSCE
                     RIVANDRLLDEGSFHTLRIDRGLVDGVIEAPYG"
     assembly_gap    1442..1521
                     /estimated_length=80
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1522..1917)
                     /locus_tag="PLV68_03175"
     CDS             complement(<1522..1917)
                     /locus_tag="PLV68_03175"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HRE00272.1"
                     /translation="MSDRSRADKRITEDEAIASLESGMTIGIGGWGSRRKPMSLVRAL
                     LRSDLTDLTVVSYGGPDVGLLAAAGKLRRVVYGFVSLDSIALEPHWRAARQAGKIAEP
                     MEYDEGMLQWGLYAGMLRLPFLPTRSGLGS"
     gene            complement(1921..2694)
                     /locus_tag="PLV68_03180"
     CDS             complement(1921..2694)
                     /locus_tag="PLV68_03180"
                     /inference="COORDINATES: protein motif:HMM:NF005925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase family protein"
                     /protein_id="HRE00273.1"
                     /translation="MGISTTIDSGGIAEVVMDVPPVNALTVAQWFQLADEITAAGRDT
                     AVRVVILRAEGRGFNAGVDIKEMQRTEGFDALIGANRGCYAAFAAVYDCPVPVIAAVH
                     GFCVGGGIGLVGNADIIIASDDATFGLPEVDRGALGAATHLARLVPQHKMRAMVYTAA
                     TATAAELHHFGSVLEVVPPTELVDAARRVAASIAAKSPAVIRAAKESLNGIDLWDVKR
                     SYRFEQGFTFELNLSGVSDELRDDFAGTNKAGDRQKADG"
     gene            2814..4274
                     /locus_tag="PLV68_03185"
     CDS             2814..4274
                     /locus_tag="PLV68_03185"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRE00274.1"
                     /translation="MGETSAAQVRAGLSHPVIDADGHFVEVAPVMNDEIIAYIEDHAG
                     KDLRDRYLSGVVAPIDTSTNLSDRGQPEIRDEWRAMPSWWGWQCRNTLDRATMHLPEL
                     LYQRLDEFGLDVTILYPSMTLSFLDMLDGELASVICRAVNAMHARMFAPYTDRMIVGA
                     LIPMSTPDMAVADLRYAREELGLRTAVIAGGTRRPIGRVHATDSALDSVAYRIDTYGI
                     DSEYDYDPFWQACMDLRVGPVSHSSFQSHRPTRSISNYVYNHVGGLAANHESLCKSLF
                     MGGVTNRFPDLRIGFLEGGVGWACSLYADMLGHWEKRRTGEIEQLDPSTLDVDAFMQF
                     VAQHGGDALNSRTDDVRAYFSRPAARPQMLDEFEPVGLTSKEDFRDKFVPNFFFGCEA
                     DDPLIGWAFRDDINPVGAKLQPLFGSDISHWDVPDMTEPLEEAYELVEHGIVDESQFR
                     EFMFLNAVKLHAAMNPDFFAGTIIEHDVTAALAAAT"
     gene            complement(4384..>4892)
                     /locus_tag="PLV68_03190"
     CDS             complement(4384..>4892)
                     /locus_tag="PLV68_03190"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRE00275.1"
                     /translation="PVNTLSPEFATEIFHRSDTLVGWFIGVYGIGAVLAGIWVSGQPL
                     AGDRQLATRQALIMVGFVVFASTSLLPVAIVGLVLGGFAFIATSAAALTRVQQRTDPS
                     EHGRLMAVWSMAFMGSRPLAGVVGGAIASFTGVRIATYVLAVPSALAASIMWRRGATG
                     ILRRTHPA"
     assembly_gap    4893..4923
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4924..5287)
                     /locus_tag="PLV68_03195"
     CDS             complement(<4924..5287)
                     /locus_tag="PLV68_03195"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRE00276.1"
                     /translation="MPILFSAALVLGISQTFSTPSMLAMVPYLVPAENVAAAVSLNIV
                     TLNVARAIGPVLGAIVVDQLGPGAAFGINAVSFMGLVVALMFVRPRPANRPPGAGRPR
                     LTDTIRGLRHQPEVATLLF"
     assembly_gap    5318..5539
                     /estimated_length=222
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5643..>6067)
                     /locus_tag="PLV68_03200"
     CDS             complement(5643..>6067)
                     /locus_tag="PLV68_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00277.1"
                     /translation="PLPDGHGVHVVGPGGMFQSGEREGVHAVWYADSTCPTCRCQLLE
                     VTAPPDHGGRGKAHSHSEDEIIYLLDGGVSLGAYTLGADTSLCIPGDIRYALAGAASG
                     HRFLNFRRDVSEQIYDRAAPPLLETGLARGGRATGDVR"
     assembly_gap    6068..6098
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6099..>6327)
                     /locus_tag="PLV68_03205"
     CDS             complement(<6099..>6327)
                     /locus_tag="PLV68_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin-like C-terminal cupin domain-containing
                     protein"
                     /protein_id="HRE00278.1"
                     /translation="LLSPPGYSLWMCDARLAPGATITYRGPHSDDALYVIDGELDVDG
                     RRCPPGGAVIVESGAEAVVTAVVESRVVHFGS"
     assembly_gap    6328..6450
                     /estimated_length=123
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6451..7532)
                     /locus_tag="PLV68_03210"
     CDS             complement(<6451..7532)
                     /locus_tag="PLV68_03210"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase family protein"
                     /protein_id="HRE00279.1"
                     /translation="MDGAVAFAPDELFFLNSTSGTTGMPKLVRHTMNRWFAFHAMAVD
                     AGEMTPDDVFMSVIPAPFGFGLWTAHFTPSILGSGCVVLERFDADEMIRAIERNKVTV
                     LAAVSTQFIMMLNSPILHDHDVSSLRVMFTGGEAVPYDRAAKFEEETGAFVLQFFGSN
                     ETGALSYTSTRDTREQRLRTAGKVIPVMQVRLFDDAGDDITATGGPGQPACRGPATCL
                     GYVDDPVADATLIRPDGWMLTGDIVEIAPTGHLSVVGRTSDFIIRGGKNISAPAVETA
                     VLAVPGVRLAAVVAMPSDIYGERVCCYVELEPGASVGLDDVRTVLAANGITKEIWPEA
                     LVVLDEMPVASGGKVAKATLREDAKGR"
BASE COUNT         1190 a         2410 c         2307 g         1059 t
ORIGIN      
        1 gcaggttgac cccgaagggc ttgcccgggg cacgctggcg gacctcgtcg atcgcatgcg
       61 ccaactggtc gaggtccatc gtcgcgctgg cgatgatgcc cagcgcccca gcgttggcgg
      121 tggcagccgt caggcgtgcg cccgacaccc agcccatccc ggtctgcacg atcggcacct
      181 gcacgcccgt gagcgtgcag aaacgagtgc ggagcgggtc gggcgtgacc ggcgcggcga
      241 gcccgtctgc cttggtcacg acacctcgcg gaagcgcaga ccctgtgggt cgaggcggtc
      301 gatcaccgcc tgctcgtcag cggtaggggc gcgcgactcg ggcacctcgg ccgggatgac
      361 cagcgggaag cccgtcgccg cgacgacctc gccgacnnnn nnnnnngggt gcaccgatcg
      421 caaccgcatc gagtgatcgg gggtctcgaa atcgagcacg gccaggttgg tgatgacccg
      481 cggcagccgg tgatggcctt gggccaccgg gctcagccgt gccacccggt cgtagcccag
      541 cccgctgacc atgtcgacgt ggtcgacgaa gatccgcgtc gagtgacggg ggatgaagaa
      601 gctggtggcg tggctgatgg tgttgcccgg cccaccacgc acaccgagca actgcacctt
      661 gggttgctgg aacggcccga tgttggcgat gttcgtgttg ccgtaccggt cgatctggct
      721 ggcgcccatc atgacgtggc ggcggcccga ccagagcatg tcgaacacgg tgcggaacgg
      781 cacccacgcc tcgaccacct tgtcggtgct gccgatgggc aggtcgttgc cgacgaagaa
      841 ggcttcgcca tcgctcacca tcaggtccgg ctcgaacgtc gccttcgccg tgcgcgcgcc
      901 gagcatctgg atcgtgccca tgccgctggc gaagatctcg ccatcgccgc ggaaggcctc
      961 ggcggcggcg atcaccacga tgtcggcgag atgtgccgat gcagactctg cgggcttcgg
     1021 ttcggtgccg gtgacggtca tgccgcgtgt tccttccatg ccaggacggc cgcctgatag
     1081 tcggcctcgg atccctgcaa gtactgccgc tcgaactcgc gccnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn accgtagggg gcttcgataa cgccgtcgac caacccgcgg tcgatgcgca
     1261 gggtgtggaa cgaaccttcg tcgagcagac ggtcgttggc gacgatccgc tcgcagctca
     1321 cgaaccggtg acccggctcg caggcaccga ggaagaggtc gtcgaagtag aggtcggggc
     1381 cgaggaattg ggcattgccg cgcgcgtcgg cgcgattcat atggacgaag gcggcgtcga
     1441 gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn ncgacccgag accggaccgc gtcggcagga acggcaggcg
     1561 cagcatcccg gcgtagaggc cccactgcag catgccctcg tcgtactcca tcggctcggc
     1621 gatcttgccc gcctggcggg cggcgcgcca gtgcggttcc agcgcgatcg agtcgagcga
     1681 gacgaacccg tacacgaccc ggcgcagctt gccggccgcg gccagcaggc cgacgtccgg
     1741 gccaccgtag ctcaccacgg tcaggtcggt caggtccgat cgcagcaggg cgcgcaccag
     1801 gctcatcggc ttgcggcgac ttccccagcc gccgatcccg atcgtcatgc cgctctcgag
     1861 cgaagcgatc gcctcgtctt cggtgatgcg cttgtcggcc ctcgaccgat cgctcatagc
     1921 tcaaccgtct gctttctggc ggtcgcccgc cttgttcgta cccgcgaagt cgtcgcgcag
     1981 ctcgtcgctg acgcccgaca ggttcagttc gaaggtgaag ccctgctcga accggtagct
     2041 gcgcttcaca tcccacagat cgatgccgtt gagcgattcc ttggcggcgc ggatcaccgc
     2101 cggcgacttg gccgcgatgc tcgccgcgac gcgacgtgct gcatccacca actcggtcgg
     2161 tggtaccacc tcaagcacgc tgccgaagtg gtggagttcg gccgcggtgg cggtcgccgc
     2221 ggtgtacacc atggcgcgca tcttgtgctg gggcaccagg cgggcgagat gggtcgcggc
     2281 gccgagcgcg ccgcggtcga cctcgggcaa cccgaaggtg gcgtcgtcgc tggcgatgat
     2341 gatgtcggcg ttgcccacca agccgatacc gccacccacg cagaacccgt ggaccgcggc
     2401 gatcaccggc accgggcagt catagaccgc ggcgaaggcc gcgtagcacc ctcggttcgc
     2461 gccgatcagg gcgtcgaagc cctcggtgcg ttgcatctcc ttgatgtcga ccccggcgtt
     2521 gaagccacga ccctcggcgc gcaggatcac cacccgaacg gcggtgtcgc gccctgcggc
     2581 cgtgatctca tcggcgagtt ggaaccactg ggcgaccgtg agagcgttca cgggaggaac
     2641 gtccatcacg acctcggcga tgccgcccga atcgatcgtc gtactgatac ccatcaacct
     2701 ctccagttca ccgggcagcc ggatcgcccc gtctctcctc gactctactc actgctatca
     2761 tcttgaagat tacaatcaca aagactacag cacgcttgaa ggagtgatgc cacatggggg
     2821 aaacgtcagc agcccaagta cgggccggac tctcgcatcc ggtcatcgat gcggacggtc
     2881 acttcgtcga agtggcgccg gtgatgaacg acgagatcat cgcctacatc gaagaccacg
     2941 ccggtaagga cctgcgcgat cgatacctca gcggtgtggt cgcaccgatc gacacgtcga
     3001 ccaacctgag cgatcgcggc cagcccgaga tccgtgacga gtggcgagcg atgccgtcgt
     3061 ggtggggttg gcagtgccgc aacacgctcg accgggcgac gatgcacctg cccgagctgc
     3121 tctatcaacg cctcgacgag ttcggcctcg acgtgaccat cctgtacccc tcgatgacgc
     3181 tgtcgttcct cgacatgctc gacggcgagc tcgcctcggt gatctgccga gccgtcaacg
     3241 cgatgcatgc ccggatgttc gcgccgtaca ccgaccggat gatcgtcggc gctctcatcc
     3301 cgatgagcac tcccgacatg gccgtcgccg atctgcgcta cgcccgcgag gagctgggcc
     3361 tgcgcaccgc cgtcatcgcg ggcggtaccc gccggccgat cgggcgggta cacgcgaccg
     3421 actcggcact cgattcggtc gcgtaccgca tcgacaccta cggcatcgac agcgaatacg
     3481 actacgaccc gttctggcag gcctgcatgg acctgcgcgt cgggccggtg tcacacagct
     3541 cgttccagtc gcaccgtccg acccgctcga tcagcaacta cgtctacaac cacgtcggtg
     3601 gcctggccgc gaaccacgag tcgctgtgca agtcgctgtt catgggtggc gtcaccaatc
     3661 gctttcccga cctgcggatc ggcttcctcg aaggcggcgt cggctgggca tgcagtctct
     3721 acgcggacat gctcggccac tgggagaagc gccgcaccgg cgagatcgag cagctcgacc
     3781 cctcgacgct agacgtcgac gcgttcatgc agttcgtcgc ccagcacggc ggcgatgccc
     3841 tcaacagccg caccgatgac gtgcgggcct acttctcccg cccagcggca cgcccgcaga
     3901 tgctcgacga gttcgagccg gtggggctga catcgaagga agacttccgc gacaagttcg
     3961 tgccgaactt cttcttcggc tgcgaagccg acgatccgct gatcgggtgg gcgttccgcg
     4021 acgacatcaa cccggtgggt gccaagctgc agcccctgtt cggttccgac atctcccact
     4081 gggacgtgcc cgacatgacc gagccgctcg aagaggcgta cgaactggtc gagcacggca
     4141 tcgtcgacga gtcgcagttc cgcgagttca tgttcctcaa cgcggtgaag ctgcacgcgg
     4201 cgatgaaccc ggacttcttc gccggcacga tcatcgaaca cgacgtcacc gccgccctcg
     4261 ccgcggccac ctgaacgccg accggtcccg ggcaccacga ggcagcgtgc ccgggaccgc
     4321 cggctcccca caacaggcgg accaatcgat cacgacagca ggggcggctt gttgggtggg
     4381 gggttatgcc gggtgggttc gacggaggat gccggtggca ccgcggcgcc acatgatcga
     4441 ggccgccaac gctgacggga cggcgagcac gtaggtggcg atgcggacgc ctgtgaacga
     4501 tgcgatcgcc ccgccgacca ccccagccag gggccgcgaa cccatgaacg ccatgctcca
     4561 cacggccatc aggcgaccgt gctcggacgg gtcggtgcgc tgttgcaccc gcgtcagagc
     4621 cgccgccgac gtggcgatga acgcaaagcc tcccagcacc agccccacta tggccaccgg
     4681 caacagactc gtggaggcga acacgacgaa gccgaccatg atcaacgcct gacgggtcgc
     4741 caattggcgg tcgcccgcca gcggttgacc ggagacccag atgccggcca acaccgcacc
     4801 gatgccgtag acgccgatga accagccgac gagcgtgtcc gagcggtgga agatctccgt
     4861 ggcgaactcc ggtgacaacg tgttgacggg atnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4921 nnncgaacag caaggtggcg acctcgggtt ggtgacggag gccgcggatc gtgtcggtca
     4981 gccgcgggcg accggcacca ggcgggcggt tggccggacg cggacggacg aacatcagcg
     5041 cgacgaccag ccccatgaac gacaccgcgt tgatgccgaa cgccgcgcct ggccccaact
     5101 gatcgaccac gatcgccccc agcaccgggc cgatggcgcg cgcgacgttg agcgtcacga
     5161 tgttcaagga aaccgccgcg gcgacgttct cggccggtac gagatacggc accatcgcca
     5221 acatcgacgg cgtgctgaag gtctgactga tgccaagaac gagggccgcc gagaacagga
     5281 tgggcaagcc gagcgatgca acggttgcca acaccgcnnn nnnnnnnnnn nnnnnnnnnn
     5341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5521 nnnnnnnnnn nnnnnnnnnc gacagcatgt tgccgacgaa gtacggggcg aagttgcggt
     5581 cggacagcgc cccgatcggg ccgacgcgcc ggggcgtgtg caccgacgcg ggctcggcga
     5641 cgtcagcgca catcgccggt cgcgcggcct ccccgtgcca gaccggtctc caacaacggc
     5701 ggagccgctc ggtcgtagat ctgctcggag acgtcgcgcc ggaagttcaa gaagcgatgt
     5761 ccgctggccg cgccggcgag ggcgtaacgg atgtcgccag gaatgcacag cgacgtgtcg
     5821 gccccgagcg tgtacgcgcc caacgagacg ccgccgtcga gcaggtagat gatctcgtct
     5881 tcggagtggc tgtgggcctt gccccggccg ccgtggtcgg gcggcgcggt cacctccagc
     5941 agttggcagc ggcaggtcgg acacgtgctg tcggcgtacc acaccgcgtg cacgccctca
     6001 cgctcgccgc tctggaacat gccacccgga ccgaccacat ggacaccgtg accatcgggc
     6061 agtggcgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncg gagccgaagt gcacgacgcg
     6121 gctctcgacc accgctgtca ccaccgcctc cgcacccgac tcgacgatca ccgcaccgcc
     6181 cggggggcag cgacgtccgt cgacgtcgag ctcgccgtcg atcacgtaca gggcatcgtc
     6241 gctgtgcggg ccgcggtagg tgatcgtcgc ccccggtgcc agccgtgcgt cgcacatcca
     6301 caggctgtaa ccgggcgggc tcaacagnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cgacccttgg cgtcttcgcg caacgtggcc
     6481 ttggccacct tgccgcccga cgcgacgggc atctcgtcga gcaccaccaa cgcttcgggc
     6541 cagatctcct tggtgatccc gttcgcggcg agcaccgtgc gcacgtcgtc gaggcccacg
     6601 ctcgcacccg gttccagctc gacgtagcag cacacccgct cgccgtagat gtccgacggc
     6661 atcgccacca ccgcggctag gcgcacaccc ggtaccgcga gcaccgcggt ctcgaccgcc
     6721 ggggcgctga tgttcttgcc gccgcggatg atgaagtcac tcgtgcgccc gaccaccgac
     6781 agatgtccag tcggcgcgat ctcgacgatg tcgcccgtca acatccagcc gtcgggacgg
     6841 atgagggttg catcggcgac cgggtcgtcg acgtagccga gacacgtggc cgggccacgg
     6901 caggccggct ggccggggcc acccgtggcg gtgatgtcgt cgccggcatc gtcgaacagc
     6961 cgcacctgca tcaccgggat caccttgccc gcagtgcgca gccgctgctc gcgcgtgtcg
     7021 cgggtagagg tgtagctgag cgcgccggtc tcgttggagc cgaagaactg cagcacgaag
     7081 gcgccggtct cctcttcgaa cttggccgct cggtcgtacg gcaccgcctc gccgccggtg
     7141 aacatcaccc gcaaggagct gacgtcgtgg tcgtggagga tcggcgagtt gagcatcatg
     7201 atgaactgcg tgctcaccgc ggccagcacc gtgaccttgt tgcgttcgat cgcccggatc
     7261 atctcgtcgg cgtcgaagcg ttcgagcacc acgcagccac taccgaggat cgacggcgtg
     7321 aagtgggcgg tccacaggcc gaacccgaac ggcgcaggga tcacgctcat gaagacgtcg
     7381 tcgggggtca tctcaccggc atcgacagcc atcgcgtgga aggcgaacca acggttcatg
     7441 gtgtgccgca ccagcttggg catgcccgtg gtgcccgacg tcgagttcag gaagaacagc
     7501 tcgtccgggg cgaacgcgac cgctccgtcc acggcccgcc cgtctggcgc
//
LOCUS       DAORNF010000050         7532 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_7221_length_7532_cov_1.947706, whole genome shotgun sequence.
ACCESSION   DAORNF010000050 DAORNF010000000
VERSION     DAORNF010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 7532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7532
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7221_length_7532_cov_1.947706"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="PLV68_03215"
     CDS             complement(<1..393)
                     /locus_tag="PLV68_03215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00280.1"
                     /translation="MTRGILDTCVLIDLHADAVSTDALPDEQAITSLSLGELSFGVVV
                     APTATDRLDRQTRLDHYRDLFRNATIPYDTAAAMLFGLVVNSVLERGKGSIRRRTVDL
                     QMAAIAVAHQLPLYTVNHTDFQGITGLTV"
     gene            complement(390..662)
                     /locus_tag="PLV68_03220"
     CDS             complement(390..662)
                     /locus_tag="PLV68_03220"
                     /inference="COORDINATES: protein motif:HMM:TIGR01552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HRE00281.1"
                     /translation="MRATLTELNNNANALARAAQAGQTVVITDRGTPIADIVPHHEQA
                     WVTKAQLTDTLSLLQATLQNPRTTSNVVREEMDAVVDPYLDIEAAR"
     gene            complement(848..1519)
                     /locus_tag="PLV68_03225"
     CDS             complement(848..1519)
                     /locus_tag="PLV68_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00282.1"
                     /translation="MLPGAVLIVLAVAGGCTQPPKGTIDVHDIQTTTTAPRPTTTAAP
                     TTTSPPTTTTLEVITTTSDDADATTTTTLDQERLEAQVAADFLDARDERTRCGREPET
                     CDFDRLALPGSAQDLYNRSIMADRIASNQRTVEGNGELQTLIESIAVSGTTATIVACQ
                     VDSLVLFDAGDLSDPSDDVILDDGTSSTRIHWDLVLDSGRWRLTGGLDLEQIEGASLC
                     ATQPD"
     gene            complement(1785..>2574)
                     /locus_tag="PLV68_03230"
     CDS             complement(1785..>2574)
                     /locus_tag="PLV68_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00283.1"
                     /translation="TATLSGGGTTVTGTTSGTGTTRNYTLNSALTWGTWTITASANGA
                     GAGSNSTAVVVNGTATATTSRDVALTARSTYYRFIVQHDSAGFNGATVAIGGVSGITA
                     GNGSNAGRVNLTVPETQHDYTVSANGYITVTDTASLGGYTAGTRLDVGPIAIYTIPTI
                     SGSTQDDEATPGPVNFTTVYLCEGAAPAATDPACGATGGPGVVATVSQGGTPGPNFTF
                     NTSGLSLAPGAYHVRGVAGTESGTVNFTITATGTALGTLTITLS"
     assembly_gap    2575..2698
                     /estimated_length=124
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2699..>3495)
                     /locus_tag="PLV68_03235"
     CDS             complement(<2699..>3495)
                     /locus_tag="PLV68_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00284.1"
                     /translation="TDITVSPQSGTYSPLPIELTATKVNITGLAQKKANTTDPAVPFA
                     SEGTATLRLESDNSVVATSTPLSASGYYSFPAVTGSGPYVVEVALSGYTSATSDSFTP
                     TLGIDDVGPTVTVAKTGGATITVTGATVAPTATASPGSLTGTCTSASTCTFTGMTAGT
                     TYTVTVTPAGADADYLWAGTTTVAPTTGETATGSVTLAQRDIVVTVGPAGASGATVTL
                     TPAGGSARNPTTSLPNSDPTTFTFARVPKSAGTITVSKTGYFTVTEQ"
     assembly_gap    3496..3505
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3506..>5371)
                     /locus_tag="PLV68_03240"
     CDS             complement(<3506..>5371)
                     /locus_tag="PLV68_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00285.1"
                     /translation="ADLTPAVVVDAVRSPDSPGTTVPAVATSSTTTGVYAITPAAAGT
                     WDLLFHAPTGYRFEGTGATTASAGPAFRLDPTDSVSRDIDLVQLATLDIAVVNADLDG
                     AGDPQPFAGSAPTVTLHRVSTGLPGDPGDESIPVPLTQVGTTGEFVATIANLPVNWVS
                     PTTVPAHYFVTITKDGYDTEIVAVTFDTDPPTEIAGTDIPVDLLAGQHTTVTLTLSRH
                     GSISGEVHGNEPLESPPVVGGPLRNPAGTTMTATRVFFIDGTTAASGPVLSFTDNPAS
                     NAYLDPVTNEFRFSGPPGYYEIALANPWYEAVAASWPTGSVTHPDLYLMSNAASLGGD
                     NVLDAAIVLDLLPTSITVHATGSGTDLTGALVALWRGGSAIDSTLTGPGGLATFADLA
                     AGAYEVRVRLQSGGEDQRFPVFVGITTQYGATAAVRDVTVTTDLVALDGEITGTVKAV
                     NSLNAPVPLPASFVVDREYKPLSPNTATEADLTGGAKPPSSYTFTPTSDATSMTIVMD
                     DLPRGTHELLFGDVGGFTSPTPDPDFPITAVIGTGAAVLTDQTYTAADVTVEITLDYA
                     ALGTDPTDGDPVVVIKPTTQGTGNYTAVHTAGTDTYTFQIPPSTAAWTVAVTHPL"
     assembly_gap    5372..5381
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5382..>7161)
                     /locus_tag="PLV68_03245"
     CDS             complement(<5382..>7161)
                     /locus_tag="PLV68_03245"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HRE00286.1"
                     /translation="QLDINATYTITASADGYLTQTRVVTPQTNVGNIDFAMVRTTGAL
                     TGNTVSSAFGDLAGVGIMVTGSDLVFGSTSAAAPSAGWFRLDEIPPGDYLITFTRYDH
                     ADVSQRVVVEAGVTLDLGEIAMEYRQRPPLTETGRLVVAVQNSANVPLGGATVALLKI
                     SDGTVVRTITDTVGTETSFAFDNVPIGTYTLRVSHGSYRTQSRRISMGLSARTETFVL
                     LQKGQVSGRVVNAVRVRAAGALGDPQLNNYEIKIYRLNLDGSRSLPELERIVVPSTAT
                     PDGDGNILFQSQPTSLTDGTYEVELSVRPVGFRRQPDQHIDPDDATSVMRFTIGDTDE
                     GSLRLNDIRVDPYPSLTGRVLEPVTAANPFKPLANATVRLTCTGQPGVATATTDAFGV
                     YTFNRRTIDEQTLLGDCSARLSASEFRQADIDQQRRLQQRQDRQHGDRPRACADRWID
                     DLARPHHRRSRADPRRRCVQQRQRVGRLLRCRRPDHGHHRGAGRSQLPPAEPTRSPLR
                     HRHRRNQHRWHVGHLRPAVRPGRLPLRRRQQVHLGHRHGHHHRRWTLGQRHRAGRHRH
                     RSVHRRHRRAAHPQSGHDRRRGLGRHG"
     assembly_gap    7162..7256
                     /estimated_length=95
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7257..>7532)
                     /locus_tag="PLV68_03250"
     CDS             complement(<7257..>7532)
                     /locus_tag="PLV68_03250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HRE00287.1"
                     /translation="IIVGPEGPLGGVDITITDGVLDFATKTSSEPGSVGRWSMSGLST
                     PNTYTLTAAMRGYGTEVQQITLGPGEQRNGLSVTMHAGVAAVSGLIT"
BASE COUNT         1039 a         2114 c         2811 g         1329 t
ORIGIN      
        1 gacggtgagt ccggtgatgc cttggaagtc ggtgtggttg accgtgtaga gggggagttg
       61 atgggcgact gcgatcgcag ccatctggag gtcgacggtg cgtcggcgga tcgagccttt
      121 gccgcgttcg aggactgagt tgacgaccag cccgaagagc atcgccgcgg cggtgtcgta
      181 gggaatcgtg gcgttgcgga acaggtcgcg atagtggtcg aggcgggttt ggcggtcgag
      241 acggtccgtg gcggtcggcg cgacgacgac accgaagctg agttcgccaa gcgacagcga
      301 cgtgatcgcc tgctcgtcgg ggagcgcgtc ggttgacaca gcgtcggcgt ggagatcgat
      361 caggacgcag gtgtcgagga tgccgcgggt catcgggcag cctcgatgtc gaggtacgga
      421 tcgaccacag cgtccatttc ttcgcggacc acgttggacg tggtccgtgg gttctggagg
      481 gtggcctgga gcaggctgag tgtgtcggtg agctgagcct tggtgaccca cgcctgttcg
      541 tggtgaggaa cgatgtcggc gatcggcgtg ccgcggtcgg tgatgaccac cgtctggccc
      601 gcctgcgcgg cgcgggcaag cgcgttggcg ttgttgttga gctcggtcaa cgttgccctc
      661 atagctcgca gcctactcag caatccagct agaagtacct agatcgtctg atgcatgtct
      721 ggatcgcgct gtggccgggc ggaggcggaa ctaccgttgc cgttctctgc gccggggtgc
      781 tcgcggccac cttgtgcgtg tgacgctggg gacagggtga ctgcagtggc aacgagtgct
      841 gctgcaatca gtctggctgt gttgcgcata gcgatgcacc ttcgatttgt tcgaggtcga
      901 gtccgccggt caggcgccaa cgaccggagt caaggaccaa gtcccaatga atgcgggtcg
      961 acgatgttcc gtcatcgagg atgacgtcgt cgctggggtc ggagaggtcg cctgcgtcga
     1021 acaggacgag gctgtcgact tggcaggcga cgatggtcgc cgtagtgccg ctgacggcga
     1081 tggactcgat gagggtctgc agttcgccgt tgccttcgac ggtccgttga ttgcttgcga
     1141 ttcgatcagc catgatcgat cggttgtaga ggtcctgcgc tgatccgggg agtgcgagac
     1201 ggtcgaagtc acaggtctcg ggttcgcggc cgcagcgtgt gcgttcatct cgggcgtcga
     1261 ggaagtcggc ggctacttgg gcttcgagtc gctcttggtc gagggtggtg gtggtcgtcg
     1321 cgtcggcgtc atcgctggtg gtcgtgatca cttccagcgt ggtggtggtc ggtggcgatg
     1381 ttgttgttgg cgcggcggtg gtcgtgggcc tcggcgctgt ggtggtggtt tggatgtcgt
     1441 ggacgtcgat ggtgcctttg gggggctggg tgcaaccacc tgcaaccgcg agcacgatca
     1501 acacggcgcc gggcaacatg gcccggagtg ctcgacttgt tctggacgtc acggcgttgc
     1561 tccttggatc accgtggccc gtccaaccac acgacttgct cggcgcactc tagaacacgc
     1621 aaccggatca cgtcgatcgg caacagcaat atgcgtccgt cgtgcaacaa ccacgctgac
     1681 ctgagtgcgc gtgggtggag gcagcggaaa tcagggcagc agattcaccg gccggcggtg
     1741 cggttgagac gtcgccgaaa cgctgcgggc ttgatgctgg gtggtcagga cagcgtgatg
     1801 gtcagggtgc cgagggcggt gccggtggcg gtgatggtga agttgaccgt gccggattcg
     1861 gtgccggcga cgcctcggac gtggtaggcc ccgggggcca gtgacagtcc ggaggtgttg
     1921 aacgtgaagt tcggtccggg tgttccgccc tgggacacgg tggcgacgac gcccgggccg
     1981 cccgtggcgc cgcaggccgg gtcggtcgcg gctggggctg caccttcgca cagatagacg
     2041 gtggtgaagt tgaccgggcc gggtgtcgcc tcgtcgtctt gggtcgagcc gctgatggtc
     2101 gggatcgtgt agatcgcgat cgggccgacg tcgaggcggg tgccggcggt gtatccgccg
     2161 agcgacgcgg tgtccgtcac ggtgatgtag ccattggccg acaccgtgta gtcgtgctgc
     2221 gtctcgggca ccgtcagatt gacccggccg gcgttcgagc cgttgccagc ggtgatgccc
     2281 gatacgccgc cgatggcgac ggtggccccg ttgaaccctg cgctgtcgtg ctgcacgatg
     2341 aagcggtagt aggtcgaccg ggccgtcagt gcaacgtcac gactggttgt cgcggtggcg
     2401 gtgccgttga ccaccaccgc ggtcgagttg ctccctgccc cggcgccgtt ggccgaggcg
     2461 gtgatggtcc aggtgcccca cgtgagcgcc gagttgagcg tgtagttgcg ggtggtgccc
     2521 gtgccgctcg tggtccccgt caccgtcgtt ccgccgcccg acagcgtggc cgtgnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncc
     2701 tgctcggtga cggtgaagta gccggtcttg ctgaccgtga tcgtgcccgc cgacttcggc
     2761 acccgcgcaa acgtgaacgt ggtcgggtcg ctgttgggca gcgaggtggt cgggttgcgc
     2821 gcggatccgc ccgcgggggt caacgtgacg gtggccccgc tggcacccgc cgggccgacg
     2881 gtgaccacga tgtcgcgctg ggccagcgtg accgaaccgg tcgctgtctc acccgtggtg
     2941 ggtgcgaccg tggtggttcc cgcccagagg tagtcggcgt cggcaccggc cggggtcacc
     3001 gtgacggtgt aggtggtgcc ggcggtcatg ccggtgaagg tgcaggtgct cgcgctggta
     3061 cacgtgccgg tgagcgagcc ggggcttgcc gtcgccgtcg gtgccaccgt ggccccggtg
     3121 acggtgatgg tggctccacc cgtcttggcg acggtcacgg tgggaccgac gtcgtcgatg
     3181 cccaacgtcg gcgtgaacga gtccgacgtc gccgacgtgt agcccgacaa cgccacctcg
     3241 accacgtacg gcccgctgcc ggtgaccgcc gggaacgagt agtagcccga cgccgacagc
     3301 ggtgtcgagg tcgccaccac cgagttgtcg gactcgagcc gcagcgtggc ggtgccttcg
     3361 gaggcgaacg gcaccgcagg gtcggtggtg ttggccttct tctgggccag gccggtgatg
     3421 ttgaccttgg tcgcggtcaa ctcgatcggc aagggcgaat aggtgcccga ctgcggcgac
     3481 acggtgatgt cggtgnnnnn nnnnntacag cgggtgggtg accgccacgg tccacgccgc
     3541 ggtgctgggc gggatctgga acgtgtacgt gtcggtgcca gcggtgtgca cggccgtgta
     3601 gttgccggtg ccctgcgtgg tcggcttgat gaccaccacc gggtcgccgt cggtggggtc
     3661 ggtgcccaag gcggcgtagt cgagcgtgat ctcgacggtg acgtcggccg cggtgtatgt
     3721 ctggtcggtc aacaccgccg cgccggtgcc gatgaccgcg gtgatcggga agtcggggtc
     3781 gggtgtcggc gaggtgaacc cacccacgtc gccgaacagc aactcgtgcg taccgcgcgg
     3841 caggtcgtcc atgacgatgg tcatcgacgt ggcgtcgctg gtgggcgtga acgtgtagct
     3901 gctgggcggc tttgccccgc ccgtgagatc ggcctcggtg gcggtgttgg gtgacagggg
     3961 cttgtactcg cggtcgacca cgaagctggc cggcagtggc acgggcgcat tgagcgaatt
     4021 gaccgccttg accgtgccgg tgatctcgcc gtcgagcgcg accaaatcgg tggtgacggt
     4081 gacgtcgcgc accgctgcgg ttgctccgta ctgggtggtg atgccgacga acacggggaa
     4141 gcgctggtct tcgccgccgc tttgcaggcg cacccgcacc tcgtacgcac cagcagcaag
     4201 gtcggcaaag gttgccagcc cgccgggacc ggtgagtgtg ctgtcgatcg ccgaaccgcc
     4261 gcgccacagc gccacgaggg cacccgtcag atcggtgccg ctgccggtgg cgtgcacggt
     4321 gatactggtg ggcaacagat cgagcacgat cgccgcgtcg agcacgttgt caccgccgag
     4381 cgaggccgca ttggacatga ggtacaggtc ggggtgggtc acactgccgg tcggccacga
     4441 cgcggccacc gcctcgtacc acgggttggc gagggcgatc tcgtagtacc cgggagggcc
     4501 gctgaagcgg aactcgttgg tgaccggatc gaggtaggca ttgctggccg ggttgtcggt
     4561 gaagctcaac acgggaccgc tcgccgcggt ggtgccgtcg atgaagaaaa cccgcgtcgc
     4621 ggtcatggtg gttcccgcag ggttgcgcaa cggtccgccg accaccgggg ggctttcgag
     4681 cggttcgttg ccgtgcacct cgccgctgat cgaaccgtgg cgcgacaggg tgagggtcac
     4741 cgtggtgtgt tggcccgcga gcaggtcgac gggaatgtcg gtgccggcga tctcggtcgg
     4801 tgggtcggtg tcgaaggtga cggcgacgat ctcggtgtcg tagccgtcct tggtgatggt
     4861 gacgaagtag tgggccggga ccgtggtcgg agagacccag ttgaccggga ggttggcgat
     4921 ggtggccacg aactcaccgg tggtgccgac ctgggtgagg gggacgggga tgctctcgtc
     4981 gccaggatcg cccggcaggc cggtgctgac acggtgcagc gtgaccgtcg gggccgaccc
     5041 ggcgaagggc tgcgggtcgc cggcgccgtc gaggtcggcg ttgacgacgg cgatgtcgag
     5101 cgtggcgagc tgcaccaggt cgatgtcgcg cgacacgctg tcggtggggt cgagccggaa
     5161 ggcggggcca gccgaggcgg tggtggcacc ggtgccttcg aagcgatagc cggtcggggc
     5221 atggaagagc aggtcccaag tacccgctgc ggcaggggtg atggcataca caccggtggt
     5281 ggtgctggat gtggccactg cgggcaccgt ggtgccgggc gagtccggcg agcgcaccgc
     5341 gtcgaccacg accgccgggg tcagatcagc cnnnnnnnnn naccgtggcg accgagaccc
     5401 cgccgtcgat catgcccgga ttgggggtga gctgcacggc gatggcggcg atggacggat
     5461 cggtgtcggt gacgaccagc ccggtgccgc tgaccgagcg tccagcgtcg gtgatggtga
     5521 ccgtgacggt gcccgaggtg tacttgctgc cgccgtcgaa gaggtagtcg gccgggccgt
     5581 acagctggcc gaagatgtcc cacgtgccat cggtgttggt tccgtcgatg ccggtggcgt
     5641 agcggggatc gggtgggttc ggcgggcgga agctgggatc gtccggcgcc acggtggtgg
     5701 ccgtggtctg gtcttcgaca ccgaagtagc cgaccaacac gttgccgctg ctggacacac
     5761 cgacgtcggg gatcggcacg agatcgccgg tggtgcggtc gagccaggtc atcgatccac
     5821 cgatcagcgc aggctcgcgg ccgatcgcca tgttgacgat cttgtcgctg ctgaagccgt
     5881 cgttgctgat cgatgtcggc ttggcggaac tcgctcgcgc tgaggcgagc gctgcagtcg
     5941 ccgagcagtg tctgttcgtc gatcgtgcgc cggttgaagg tgtagacacc gaaggcgtcg
     6001 gtggtggcgg tcgcgacacc cggctgcccg gtgcaggtga ggcgaaccgt tgcattggcc
     6061 aggggcttga acgggttggc cgcggtgacc ggttcgagca cccgcccggt caggctggga
     6121 tacgggtcga cgcggatgtc gttgagccgg agcgaccctt cgtcggtgtc gccgatggtg
     6181 aagcgcatca ccgacgtggc gtcgtcgggg tcgatgtgct ggtcaggttg ccgccggaaa
     6241 cccacggggc gaaccgagag ctcgacctcg taggtgccat cggtcagcga ggtgggctgg
     6301 ctttggaaga ggatgttgcc gtcaccgtct ggggtggcgg tgctcggcac cacgatccgc
     6361 tcgagctcgg gcaggctgcg cgacccgtcg agattgagcc ggtagatctt gatctcgtag
     6421 ttgttgagct gcggatcgcc gagtgctccg gcggcacgga cccgcacggc gttgacgacg
     6481 cgcccggaga cctggccctt ctgcaagagc acgaatgtct cggtgcgtgc gctgagaccc
     6541 atcgagatgc gccgcgactg tgtgcggtag ctgccgtggc tgacgcggag ggtgtaggtg
     6601 ccgatcggaa cgttgtcgaa cgcgaagctg gtctcggtgc cgaccgtgtc cgtaatggtg
     6661 cgcacgaccg ttccgtccga gatcttcaac aacgcgacgg tcgccccgcc gagcgggacg
     6721 ttggcgctgt tctgcacggc cacgacgagg cggccggtct cggtcagtgg tgggcgctgg
     6781 cggtattcca tcgcgatctc gccgagatcg agggtgaccc ctgcttcgac cacgacccgt
     6841 tggctgacgt cggcgtggtc gtagcgggtg aaggtgatga ggtagtcgcc cggcgggatc
     6901 tcgtcgaggc ggaaccatcc ggcgctgggg gccgcggccg acgtcgaccc gaagacgaga
     6961 tcgctaccgg tgaccatgat gcccaccccg gccaggtcgc cgaaggcgct cgacaccgtg
     7021 ttgccggtga gggcgccggt ggtgcgcacc atcgcgaagt cgatgttgcc cacgttggtc
     7081 tgcggggtga ccacccgcgt ctgggtgaga tacccgtcgg cgctggcggt gatggtgtac
     7141 gtcgcattga tgtcgagttg gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntggt
     7261 gatcaggccg gacaccgcgg cgaccccggc gtgcatcgtg acgctgagcc cgttgcgttg
     7321 ttcgcccggg ccgagcgtga tctgctgcac ctcggtgccg tagccacgca tcgccgcggt
     7381 gagcgtgtag gtgttgggtg tgctgagccc gctcatcgac cagcgtccga ccgagcccgg
     7441 ctcgctcgac gtcttggtgg caaagtccaa cacgccgtcg gtgatggtga tgtcgacacc
     7501 gccgaggggg ccttcggggc cgacgatgat gc
//
LOCUS       DAORNF010000051         7529 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_7226_length_7529_cov_1.619481, whole genome shotgun sequence.
ACCESSION   DAORNF010000051 DAORNF010000000
VERSION     DAORNF010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 7529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7529
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7226_length_7529_cov_1.619481"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(162..>1053)
                     /locus_tag="PLV68_03255"
     CDS             complement(162..>1053)
                     /locus_tag="PLV68_03255"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRE00288.1"
                     /translation="TVTGSATAPDLATTVPSVASHPNDPTDGEALVRYLDQIADQPRL
                     DASDERRLSAAIVAGHDAERQLQGDPFITDDAREVLLSVIASAADARRSLTQSSLRVV
                     VDIAARSGRHGHDLLDLIQHGNAGLLRAADRFDASSGTSFSAYAAWWIRQAINEMPDP
                     TGPIPRPTDPTWWDQLERERLDDTPPAPRRPPIATTGSDGHGHGHGSDTLSPYEQSAE
                     ALERQTLQMQLRRLRPAERAAVVSRLGLGDAIELDEADATSTRGMSRTHLRTVEELAL
                     SKLRHPTARRPWTNAPSTAV"
     assembly_gap    1054..1093
                     /estimated_length=40
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1095..1161
                     /locus_tag="PLV68_03260"
     tRNA            1095..1161
                     /locus_tag="PLV68_03260"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1122..1124,aa:Val,seq:tac)
     gene            1469..1795
                     /locus_tag="PLV68_03265"
     CDS             1469..1795
                     /locus_tag="PLV68_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00289.1"
                     /translation="MRATPNVDKDIVTVAPHYRDDTTWELFRCGKSRYLAVLPTRGEP
                     RARRDAVARMLCNVHLGSLWTATMSQEVRQESVRRARVMAVDRYRADPGNLLDDLEAS
                     LGDQCP"
     gene            1786..>2212
                     /locus_tag="PLV68_03270"
     CDS             1786..>2212
                     /locus_tag="PLV68_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00290.1"
                     /translation="MPLASTRLCGPKRSHSATYATTSRSGEGYCTTHYTELIRDKVRP
                     EAYGGKGIGLQFGPLRPDYGPGIADLQCTCCDATWTGLIGERCTFCSDNLEVQRNYQV
                     ELILRRPDMDRTDVRYPAALEAWAIRLANAVNAGRITLQQ"
     assembly_gap    2213..2231
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2232..2465
                     /locus_tag="PLV68_03275"
     CDS             <2232..2465
                     /locus_tag="PLV68_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00291.1"
                     /translation="AAVGHMTVLDPLATLHRLTADDEHGGVTECDTPDDHAASVDDRE
                     FGHLPESFWRARPILQIRSPSSPLAHPAGTYDF"
     gene            2483..>2861
                     /locus_tag="PLV68_03280"
     CDS             2483..>2861
                     /locus_tag="PLV68_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00292.1"
                     /translation="MPSKSKVDRWAVPEVKKRGSLEGKAIGFCEKRLLIPDSRQPLKL
                     HPYQRALFEEWADPSNLCHATVIGAGNAKTTTLAAFARACMCLARRTTQCALSTSSTL
                     VVRGVRHGGPGRQARPPEGWRRSS"
     assembly_gap    2862..2871
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2872..3848)
                     /locus_tag="PLV68_03285"
     CDS             complement(<2872..3848)
                     /locus_tag="PLV68_03285"
                     /inference="COORDINATES: protein motif:HMM:NF024706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HRE00293.1"
                     /translation="MKRDGDPTSQFLVYRDEAGRARIDVRFDGDTVWLTQAQLAELFG
                     TTPQNMTQHIAAVYDDGELEQKATCKEYLQVRREGSRNVQRRLVHYNLDMIISVGYRV
                     RSKTATAFRTWATQRLREYIVKGFVLDDERLANPHLPFDYFDELLERIQAIRTSERRF
                     YQKITDVYATSIDYDPGAEITQAFFATVQNKLHWAITGQTAAEVVRDRADADKPNMGL
                     TSWRGERIKRSDVGIAKNYYSRDELSALNNLVEQYLVFADGQARRRIPMKMSDWIAKL
                     DGFLTLNDRDVLTSAGKVSHELAQSHAEGQFERFTHREASAIESDFDRF"
     gene            complement(3912..>4296)
                     /locus_tag="PLV68_03290"
     CDS             complement(3912..>4296)
                     /locus_tag="PLV68_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00294.1"
                     /translation="LGNGRYSVFDNATSIDRASRMIVFSLAGDVATIERVIDDPTGSN
                     SAGQGSAGALDDAEQYWVINWGCSATGLSVMAADGTEVARLRRATATPMPDTRATVED
                     SLDFTVSYRAKVLTPTLISPSPAQP"
     assembly_gap    4297..4469
                     /estimated_length=173
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4642..>5363)
                     /locus_tag="PLV68_03295"
     CDS             complement(4642..>5363)
                     /locus_tag="PLV68_03295"
                     /inference="COORDINATES: protein motif:HMM:NF018604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HRE00295.1"
                     /translation="RHLVSGSLWVAMGLAVTAGYVDAFVYRNVMPVFVANMSGNLIKF
                     GMAIGDVDVHAVVAAAIALAAFSLAATAGAAVIDRRLSLSSRAVVDPAPLLLAEVVLL
                     TALTLVAVIAHPTLSERLTGDHILIVAFGSAAMGLQAVSLRRVGDVAVSTTYGTGAIV
                     RMAEKTALAFRRSARDHDVPRRHSVLVLSCILAWYLIGAAIAAAIAEHRALLGILPAI
                     NLLIAWRVHHIDRRGLRKTDI"
     assembly_gap    5364..5445
                     /estimated_length=82
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5446..6519)
                     /locus_tag="PLV68_03300"
     CDS             complement(5446..6519)
                     /locus_tag="PLV68_03300"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRE00296.1"
                     /translation="MAKRRTVSSGPSLALYGAGMISIAHAAAARINRIPITAVASRSA
                     ERAQNQAQRLHVPAAAYADLPGDASIVAVSTPPQCHAADALAMLASGAAVVLEKPLCT
                     TLADADALVDAAGHRLLYAESLAYSPLVQAMLAAVPRLGAVERVEVRTIQSLPTWGEF
                     TSDAWGGGALFDLGVHPLAVALLVAGAAGAGQPRSVTCTLRGGPGHNSDEHAEVAITF
                     ANGLVALVTASWQAARVLGRDGQVWDVQASSPTGVLRGDFFPTPTLEHNGDPVALAGV
                     ADEQAAVIEGFGYAGQLRSFVADLAAQRTPLMDAAFGRFVLDIVCAAYRSAGREGAAE
                     ALPFRGDRTLTPLQLWRGGPTAD"
     gene            complement(6525..>6991)
                     /locus_tag="PLV68_03305"
     CDS             complement(6525..>6991)
                     /locus_tag="PLV68_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HRE00297.1"
                     /translation="KPVQARPPILIGGGGPRVLSIAGREADIVGINGTMTAGVVGPDA
                     IATMTAASVDDKVGTVRRAAGERCDQIEMNIRVFLMTFTDDRAGAMAAIGGAVGMDTS
                     FVEQSPFALIGTPEQMVADLRARRDRWGFSYVIIGAEHVEAFAPVAAELRGN"
     assembly_gap    6992..7001
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7002..7469)
                     /locus_tag="PLV68_03310"
     CDS             complement(<7002..7469)
                     /locus_tag="PLV68_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HRE00298.1"
                     /translation="MSSTKPFRFGVQASTATTAEGWVDLARRCEALGYDVLTMPDHFG
                     DQLAPIPALMTAANATTTLRVGALVLDNDYKHPLVLAKELATMDVLSGGRVEVGLGAG
                     WLVTDYEQSGIAYDSPKVRIDRFAEGLAVIKGCFGAGPFSFAGEHYTITDHDAL"
BASE COUNT         1134 a         2360 c         2512 g         1189 t
ORIGIN      
        1 gccgtggccg acgggctggg acgcgagctg ttggtgcgcg acccgtcggt caagcgcacg
       61 cccgtcggtg ccgcgacgcc cgaactgctt gaccgcttga ccaccgtgcg gcgctggttc
      121 tgatcacccg gcccgtcgct gggctgtcgc tggtcggtgg ttcagactgc agtcgagggc
      181 gcgttggtcc acggacgtcg tgccgtgggg tggcggagct tgctcagcgc cagttcctcg
      241 accgtgcgca ggtgagtacg cgacatgccc cgagtgctgg tggcatcggc ctcgtccagc
      301 tcgatcgcgt caccgaggcc gagccggctc accactgcgg cacgctctgc tgggcgtagc
      361 cggcgtagtt gcatctgcaa cgtctggcgt tcgagggctt ctgccgactg ctcgtaggga
      421 gagagcgtgt cgctcccgtg gccgtgcccg tggccgtcgc tgccggtggt cgcgatcggt
      481 ggacgtcgcg gtgccggtgg tgtgtcgtcg aggcgttccc gttccagctg gtcccaccag
      541 gtcggatcgg tcggacgagg gatcggcccg gtggggtccg gcatttcgtt gatcgcctgg
      601 cgaatccacc acgcggcgta ggcgctgaag ctcgtacccg acgaggcgtc gaagcgatcg
      661 gcggcgcgca gcaacccggc gttgccgtgc tggatcaggt cgagcagatc gtgcccgtgc
      721 cgtccactac gggcagcgat gtcgaccacc acgcgcaggc tcgactgggt caagctgcgc
      781 cgcgcgtcgg ctgccgaggc gatgaccgag agcagcacct cgcgggcgtc gtcggtgatg
      841 aacgggtcgc cctgcagctg ccgctcggcg tcgtggcccg ccacgatcgc ggctgacaac
      901 cgccgttcgt cgctcgcgtc gagccgcggc tggtcggcga tctggtcgag gtagcgcacg
      961 agcgcctcgc catcggtcgg gtcgttgggg tgcgacgcca cgctcggcac cgtagttgcc
     1021 agatccggcg cggtggcgga ccccgtaacg gtcnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnncatagct cagttggtag agcactgccc ttacaaggca tcggtcgggg
     1141 gttcgagtcc ctctgcgccc actgcagttc ccaggtcaga gctagcgtga ggaagccgcc
     1201 accccggctg gtgagcaacg gggtgggcag taggtccagg cctaattggt cgtggcctgc
     1261 acggcctgcg gcgacactcc catggcgttg ccgagcgact tcaatgttgc gcggggtgcg
     1321 tggccacaga gttcgagttt cggtcgctgc tccacagcgt gaggaagctg tcggttgagc
     1381 agttgtgcat gttggttggg cggccacgca ggcgggttcc tggcgtgata tcgacgaacg
     1441 cgatccgtgg tcggtgctca tcctccacat gagggcgacg ccgaacgtcg acaaggacat
     1501 cgtcacggtc gcgccgcact accgggacga cacgacctgg gaattgttca ggtgtggcaa
     1561 gtcccggtat ctcgccgtgt tgccgacgcg gggagagccg agggcacggc gagatgctgt
     1621 ggcgaggatg ctctgcaacg tgcatctcgg ctcgctgtgg actgcgacga tgtcgcagga
     1681 ggtccggcag gagtcggtcc gccgagcgag agtgatggct gtggatcggt accgcgccga
     1741 tcctggcaac ctgctcgacg acctggaggc cagcctgggt gaccagtgcc cctagcttcg
     1801 acgcgcctgt gcggcccaaa gcggtcccac tcagcgacgt acgcgactac atcccgatcg
     1861 ggcgagggct actgcaccac ccattacacc gagctgatcc gagacaaggt tcgaccggag
     1921 gcgtacggcg ggaaaggcat cggcctgcag ttcgggccgc tgcgacccga ctacggcccc
     1981 ggtatcgcgg atctgcagtg cacctgctgc gacgccacct ggaccggcct gatcggtgaa
     2041 cggtgcacct tctgctccga caacctggaa gtgcagcgca actaccaggt cgagctgatc
     2101 ctgcggcgtc ccgacatgga ccgcaccgac gtccgctatc cggcggcact tgaggcgtgg
     2161 gcgatccggc tcgccaacgc tgtcaacgct ggtcggatca cgctccaaca ggnnnnnnnn
     2221 nnnnnnnnnn ngccgcagtc ggccacatga cggtgctgga cccgctcgcg accctccatc
     2281 gcctcacggc cgacgacgag cacgggggcg taacggaatg tgatacccct gacgaccatg
     2341 cggcatcggt cgatgatcgc gagttcgggc acctccctga gtcgttctgg cgggcacgcc
     2401 cgatcctgca aatacgttcg ccaagcagcc cacttgcgca tccggccggc acctacgatt
     2461 tctgacacac agggcggcgc cgatgccgtc gaagtcgaag gtggaccggt gggcggtgcc
     2521 ggaggtgaag aagcggggct cgctggaggg taaggcgatc gggttctgtg agaagcggtt
     2581 gttgatcccg gactcgcgtc agccgttgaa gctgcatccg tatcagcggg cgctgttcga
     2641 ggagtgggcg gacccgtcga atctgtgtca tgccacggtg atcggcgcgg ggaacgcgaa
     2701 gacgacgacg ttggccgcgt tcgcgagggc gtgcatgtgt ctggcccgga ggaccacgca
     2761 atgtgcgttg tccacatcct cgacacttgt cgttcggggt gtgcgacatg gcgggccggg
     2821 tcgtcaggcc agacctcctg aggggtggcg gcgttcgagc annnnnnnnn nctgaagcgg
     2881 tcgaagtcgg actcgatcgc cgacgcttcg cgatgagtga aacgttcgaa ctgtccttcg
     2941 gcgtgtgact gggctagctc gtgcgagact ttgcctgcgg aggtcagcac gtcgcgatcg
     3001 ttgagcgtca ggaagccatc cagcttggcg atccagtcgg acatcttcat cgggatgcgc
     3061 cgccgagcct gtccatcggc gaacaccagg tactgctcga caaggttgtt cagcgccgac
     3121 agctcatcgc gggagtagta gttcttcgcg atcccgacgt cggaccgctt gatccgctcg
     3181 ccacgccacg atgtcaaccc catgttcggt ttgtcggcgt cggcgcgatc acgcaccacc
     3241 tcggccgcgg tctgaccagt gatggcccag tgcagcttgt tctgcaccgt tgcgaagaac
     3301 gcctgggtga tctcagcccc cgggtcgtag tcgatcgagg tggcatagac gtcggtgatc
     3361 ttttgataga agcgacgctc cgacgtgcgg atcgcctgaa tccgctccag cagttcgtcg
     3421 aaatagtcga acggcaggtg tgggttggcc agccgctcat cgtcaagaac gaaccccttg
     3481 acgatgtact cgcgcaatcg ctgggtcgcc caagtacgga acgccgtcgc tgtcttggac
     3541 cgcacccggt agccgactga gatgatcatg tcgaggttgt agtggaccaa cctgcgctgg
     3601 acgttacgcg agccttcacg tcgaacttgt aagtactcct tacaagttgc tttctgctcc
     3661 agttcaccgt cgtcgtagac cgccgcgatg tgctgggtca tgttctgcgg ggtcgtgcca
     3721 aacaactccg cgagttgagc ctgcgtcagc cacaccgtgt caccatcgaa ccgcacgtcg
     3781 atccgcgctc gaccagcctc gtctcggtag acgagaaact ggctcgtcgg atcgccatcg
     3841 cgcttcacgc tctggagcgt acgcgcgggg tgtcgcaccg tgacactgcg cggcggcggc
     3901 cgtcgtgggt cttacggttg agcgggtgag ggggagatca gggtgggggt gaggactttt
     3961 gctcggtagg agacggtgaa gtcgagggag tcttcgacgg tggcgcgggt gtccggcatg
     4021 ggtgtggcgg tggcgcggcg gaggcgggcg acctcggtgc cgtcggctgc catgacggac
     4081 aggccggtgg cgctgcagcc ccagttgatc acccagtatt gctcggcgtc gtcgagcgcg
     4141 ccggccgagc cttggcccgc tgagttggag ccggtggggt cgtcgatcac gcgctcgatc
     4201 gtggcaacgt caccagcgag ggagaagacg atcatccgac tcgcgcggtc gatcgaggtc
     4261 gcgttgtcga acacggagta gcgaccgttg ccgagcnnnn nnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc acgaccacgt tgccgacgtc gtcgagatcg
     4501 acactgttgc agtggaatgg atcgtccgcc gcggcgacgg ctcgtgtccg catttgctcc
     4561 cagaggccgt ctggcagatg gtcgaacagg ctccaccgcc aatcgatcgc gcccgccgcg
     4621 tcgacatgcg ccacgatggc gtcagatgtc ggttttgcgc aggccgcggc ggtcgatgtg
     4681 gtggacccgc caggcgatca gcaggttgat ggcgggcagg atgcccagca gggcccgatg
     4741 ctccgcgatt gccgcggcga tcgccgcgcc gatcaggtac caggcgagaa tgcacgacaa
     4801 caccaacacg ctgtgccgtc gcggcacgtc gtgatccctc gccgagcggc ggaaggcgag
     4861 tgccgtcttc tccgccatgc gcacgatcgc tccggtgccg taggtggtcg acaccgccac
     4921 atcgccgacc cggcgcagcg acaccgcctg caggcccatc gcggccgaac cgaaggccac
     4981 gatcaggatg tggtcgcccg tcagccgctc ggacagcgtg gggtgggcga tcacggccac
     5041 cagcgtcagc gcggtcagca ggaccacctc ggccagcagc agcggtgcgg ggtcgacgac
     5101 cgccctcgac gacaggctca ggcggcggtc gatgacggcg gcgccggcgg tcgcggccag
     5161 ggagaacgcc gcgagcgcga tcgcggccgc gacgacggca tggacgtcga catcgccgat
     5221 cgccatgccg aacttgatca ggttgccgct catgttggct acgaagacgg gcatcacgtt
     5281 gcggtacacg aacgcatcga cgtagcccgc ggtgacggcc aaacccatgg caacccagag
     5341 cgatccagaa acgaggtggc gtgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntcagt cggcggtggg
     5461 gccgcctcgc cacagttgca gcggggtgag cgtgcgatcg cctcggaatg gtagagcctc
     5521 ggcggcaccc tcgcgtccgg ctgatcgata cgcggcgcac acgatgtcga gcacgaaccg
     5581 gccgaacgcc gcgtccatca acggcgtccg ctgcgcggcc aggtcggcga cgaacgaccg
     5641 cagttgaccc gcgtagccga acccctcgat cactgccgcc tgttcgtcgg ccacgcccgc
     5701 cagcgcgacc ggatcgccgt tgtgttcgag cgtcggtgtc ggaaagaagt cgccgcgcag
     5761 cacgccggtg gggctcgacg cctgcacgtc ccacacctgg ccgtcgcggc cgagcacccg
     5821 cgcggcctgc caacttgcgg tcaccaacgc gaccagtccg ttggcaaagg tgatcgcgac
     5881 ctcggcgtgc tcgtcgctgt tgtgtcccgg cccaccgcgc agcgtgcacg tgaccgacct
     5941 gggctgaccg gcacccgccg ccccggcgac gagcagggcg acggccagcg ggtgcacgcc
     6001 gaggtcgaac aaagcaccgc cgccccatgc gtcggacgtg aattcccccc acgtcggcaa
     6061 cgactggatg gtgcgtacct cgacccgctc caccgcgccc aggcgcggca ccgcggccag
     6121 catcgcctgg accagcggcg agtaggccag gctttctgcg tacagcagcc ggtgacccgc
     6181 ggcgtcgacc agcgcgtcgg cgtcggccag cgtggtgcac agcggctttt ccaacacgac
     6241 ggccgcgccg ctcgccaaca tcgccaacgc gtccgcggcg tggcactgcg gtggcgtcga
     6301 cacggcgacg atcgaggcgt cgcccggcag atcggcgtag gcggcagcag ggacgtgcag
     6361 gcgttgcgcc tggttctgcg cccgctcggc cgaccgcgac gcgaccgcgg tgatcgggat
     6421 gcggttgatc cgcgccgctg cggcatgggc gatcgagatc atgcctgctc cgtacaacgc
     6481 aagcgagggc ccagatgaga cggtgcggcg cttggccacg gcgctcagtt cccgcgcaac
     6541 tcggcggcca cgggtgcgaa cgcttcgacg tgctcggctc cgatgatgac gtagctgaag
     6601 ccccaccggt cgcgccgggc acgcaggtcg gccaccatct gctcgggtgt gccgatgagc
     6661 gcgaacggcg actgctcgac gaacgacgtg tccatgccga ccgcaccacc gatggccgcc
     6721 atcgccccgg cccggtcgtc ggtgaaggtc atcaggaaca cccggatgtt catctcgatc
     6781 tggtcgcacc gctcgcccgc cgcgcggcgg actgtgccga ccttgtcgtc gaccgaggcg
     6841 gccgtcatcg tcgcgatcgc gtcgggaccg acgacgccgg cggtcatcgt gccgttgatg
     6901 ccgacgatgt cggcctcgcg tccggcgatc gacaacaccc gcggcccgcc gccaccgatc
     6961 aggatcggcg gccgagcctg caccggcttg gnnnnnnnnn ntaacgcgtc gtggtcggtg
     7021 atcgtgtagt gctcgccagc gaacgagaac ggccccgcac cgaagcatcc cttgatcacc
     7081 gcgagaccct cggcgaagcg gtcgatgcgt accttgggcg agtcgtaggc gatgccgctc
     7141 tgctcgtagt cggtcaccag ccaccctgca ccgaggccga cctcgacccg gccgccggac
     7201 agcacgtcca tcgtggccaa ctccttggcc agtacgagcg ggtgcttgta gtcgttgtcg
     7261 agcacgagcg cgccgacgcg cagcgtggtg gtggcgttgg cggcggtcat cagcgctggg
     7321 atcggcgcga gctggtcgcc gaagtggtcg ggcatcgtca acacgtcgta gccgagcgcc
     7381 tcgcagcggc gggccaggtc gacccacccc tcggcggtgg tggcggtgct ggcttggact
     7441 ccgaagcgga acggcttggt cgacgacatg gacgccacgg tattgcacgt cccccgcccg
     7501 gaccgtgccc ggtgcgctcc acgagcggc
//
LOCUS       DAORNF010000052         7519 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_7242_length_7519_cov_1.734191, whole genome shotgun sequence.
ACCESSION   DAORNF010000052 DAORNF010000000
VERSION     DAORNF010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 7519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7519
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7242_length_7519_cov_1.734191"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="PLV68_03315"
     CDS             <1..298
                     /locus_tag="PLV68_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HRE00299.1"
                     /translation="PKDRHVLAAAVRADAAAIVTFNVADFPAPSVDPFEIDIIRPDTF
                     LLDQLDLAPGVVIDELGRQAAANRREPKTLPSILDALAKAGAPSFADEVRRRTA"
     gene            complement(325..1206)
                     /locus_tag="PLV68_03320"
     CDS             complement(325..1206)
                     /locus_tag="PLV68_03320"
                     /inference="COORDINATES: protein motif:HMM:NF019976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease-related domain-containing protein"
                     /protein_id="HRE00300.1"
                     /translation="MTQLIETRWTRYGKDRVYVKTADGDDVGHVDLVARTVVARVPDY
                     EIELQMCLARWTSAADQLPDETPVPAPPAPVGDCVTTPEPTITEPSIVEAAARDLAGA
                     AARAKRNEVNAQAPVMNFVARVLGVKTDERAWRVGAKGEEKVANELAKLDGGWRVLHA
                     VEVGENGSDIDHVLIGPPGVFTLNTKRHPRGKAWVGDRMVMVNGQPTDYLRNSRFEAN
                     RSSRLLTAACARDVPVTAAIVFVDLDGFTVKQIPPDVHVTTCRRLLGWLRALPATIDA
                     TDVEVIFAKARLSSTWK"
     gene            1290..1478
                     /locus_tag="PLV68_03325"
     CDS             1290..1478
                     /locus_tag="PLV68_03325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00301.1"
                     /translation="MSTSAGAPSQEQIEAAIQQTLVGWCAELDTGRKFWWGDWNRMIR
                     DVITAQRTGEPVLYDDDE"
     gene            2287..>2641
                     /locus_tag="PLV68_03330"
     CDS             2287..>2641
                     /locus_tag="PLV68_03330"
                     /inference="COORDINATES: protein motif:HMM:NF020873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein"
                     /protein_id="HRE00302.1"
                     /translation="MAAPMLPVQLVEARRTPLFDFATLPDALAGSHRTTVWAELRVQA
                     GSVRYVDLEGDDPRDERLVAGESVVIVPGIEHQVEPSSDAQFYVQFFREPDAPMVPGV
                     EPPASVDRSGPWMHRG"
     assembly_gap    2642..2787
                     /estimated_length=146
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2788..3035
                     /locus_tag="PLV68_03335"
     CDS             <2788..3035
                     /locus_tag="PLV68_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00303.1"
                     /translation="HVLLYAPGYDIDVIEVHRHLHGSAPFTSELFDRWLQVFHDTVAG
                     GWVGPKATMANKRATGMAWAMAQRFLGHGVWRPADRR"
     gene            complement(3068..3739)
                     /locus_tag="PLV68_03340"
     CDS             complement(3068..3739)
                     /locus_tag="PLV68_03340"
                     /inference="COORDINATES: protein motif:HMM:NF013488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HRE00304.1"
                     /translation="MLESQPVELIVYGDFNCPFSALASARVSSLERIGAVRVDWRAVE
                     HAPDLPPAGIEIAGELASEIQRELAQIRGLLSVDEPDRLRLPARQPNTRLATAAFAAT
                     PIADRPAVRDRLFAAHWSEGADLTDDTTLARLGATTIHTATAARWREEWEHLTDPIVP
                     VMVLPDGYVSRGLGALARLAKLARDTNTSGADAPDVIRPPIADHHDQGGESPCFAHLL
                     DEPPR"
     gene            3850..>4982
                     /locus_tag="PLV68_03345"
     CDS             3850..>4982
                     /locus_tag="PLV68_03345"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HRE00305.1"
                     /translation="MTTFLLPTDPIVSIDDYLANGVAGSGIRRAHELGPKATIDLVTQ
                     SGLRGRGGGGFPTGQKWAGVAAHTGGRRYLVCNGAEGEPGTFKDRAMLRANPYQLVEG
                     LAIAAFAIGATEAFVCLKASFPREIDAVTRAVQEFQSAGLCGDCKVTIVAGPEEYLFG
                     EEKAMLEVIEGNEPLPRWLPPYLHGLFATAPQLGWQSHDTEAGRSGGGGGSNPTLVNN
                     VETLSTVAHILARGAEWFRSMGTVESPGTVVATVVGDVVAPDVGEVEMGTSLRDVIDA
                     VGSGLAEGRSVKAVFSGVANAVVTAADLDVPVSYEGFQAIGSGMGSAGFIVYDDTTCM
                     VDASYRFSRFLSIESCGQCPPCKLGSSAITEHLERIETGVGDTG"
     assembly_gap    4983..5145
                     /estimated_length=163
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5361..6008
                     /locus_tag="PLV68_03350"
     CDS             5361..6008
                     /locus_tag="PLV68_03350"
                     /inference="COORDINATES: protein motif:HMM:NF024248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRE00306.1"
                     /translation="MSALQTQARALGDPTRHELFRYIVDAGRPVDVVELTDHLELHHN
                     AIRQHLAKLVDAGLVVEARAPRVGRGRPRLVYTVDPAAESRWGVTGPYERLSLLLSEI
                     IRTGDSPVEVGRRAGRQVPAAVRDTTDPVVGLVDAMERHGFEPTAKRRGNQVEIVLGA
                     CPFATTALADPETVCGLHLGLAHGAAEQLGGIVIDELRPRDPRRGACRLRCHVAT"
     assembly_gap    6173..6270
                     /estimated_length=98
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6453..>7519
                     /gene="ric"
                     /locus_tag="PLV68_03355"
     CDS             6453..>7519
                     /gene="ric"
                     /locus_tag="PLV68_03355"
                     /inference="COORDINATES: protein motif:HMM:TIGR03652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein"
                     /protein_id="HRE00307.1"
                     /translation="MQTSVRIDGASCPTCFNETIDALSQLDGVRRVHGSFAGPCLEID
                     HDIPLETINSTIRGRLHGVEIFANEIRMVPLEPAPLTTTCVHHQPEVADPTAPPDSGG
                     NTVDPSMTLGEIVTRRPVLAAELERRGLDYCCHGDRTLTDAALEAGLDARTVADELSA
                     VAVDAPAAAWASLGPSELVEHIDAVHHRYLWAELPRVTALVDKIASVHGERHPELFEV
                     QRLYGELRADLEPHLTREEQELFPPIRQLAGASDPSSVSTRAFAAQIETLADEHETVG
                     ALLEELRRVTSGYSVPPDGCASYAACYRALADLEADTHLHVHKENNLLFPAVLKTRLG
                     VDEVRQHGQAPFAQRQEQERRG"
BASE COUNT         1148 a         2298 c         2437 g         1229 t
ORIGIN      
        1 tcccaaggat cgccacgtgc tcgccgccgc cgtgagggcc gatgcggcgg cgatcgtgac
       61 cttcaacgtg gccgacttcc cggccccgag cgtcgacccg ttcgagatcg acatcattcg
      121 cccggacact ttcctgctcg atcaactcga cctggcgcct ggcgtcgtca tcgatgagct
      181 tggtcggcaa gccgcagcga accgcagaga accgaagacg ctgccgagca tcctcgatgc
      241 actcgcgaag gcgggcgcgc cgtcgttcgc tgacgaggtt cgccggcgta ctgcatgagc
      301 ggcacggcgc gcagtgagcg gctgctactt ccaggtgctg ctgagcctcg ccttggcgaa
      361 aatcacttcg acatcggttg cgtcgatcgt cgccggcagc gctcgcagcc agccgagcag
      421 gcgccgacat gtcgtgacgt gaacatccgg cgggatctgt ttcaccgtga agccgtcgag
      481 gtcgacgaac acgatggccg cggtgaccgg tacatcccgg gcgcacgcag cggtcaggag
      541 tcgtgaggag cggttcgcct cgaaccgcga gttccgcagg tagtcggtcg gctgaccgtt
      601 caccatcacc attcggtcgc cgacccaggc cttcccgcgc gggtggcgct tggtgttgag
      661 tgtgaagact ccgggaggcc cgatcaggac gtggtcgatg tcgctgccgt tctcgccgac
      721 ctccactgcg tggagcaccc tccacccgcc gtcgagcttg gccaactcat tggcgacctt
      781 ctcctctccc ttggcgccga cccgccatgc gcgttcgtcc gtcttcacgc cgagcacgcg
      841 agcgacgaag ttcatcaccg gtgcttgcgc gttcacctcg ttgcgcttgg ctcgggctgc
      901 ggcgcccgcg agatctctgg cggccgcctc gacaatcgac ggttcggtga tagtcggctc
      961 cggggtcgtc acgcagtctc cgaccggcgc gggtggagcg ggaaccgggg tttcgtcggg
     1021 gagttggtcg gcggcgctcg tccaccgcgc aagacacatc tgcagttcga tctcgtagtc
     1081 cggcaccctg gcaacgacgg ttcgagcgac gaggtcgaca tgaccaacgt cgtcgccgtc
     1141 ggcggtcttc acgtacacgc ggtccttgcc gtagcgggtc caccgtgtct cgatcagctg
     1201 ggtcacgaat tctctatcgg cgtgtggact gcgatgcacg atcgaaaagc ctgcttcgcc
     1261 catcagaccc gccgcctacc gtggctcgca tgtccacgtc cgccggcgcc ccgagccagg
     1321 agcagatcga ggccgccatc cagcagaccc tcgtcggttg gtgcgcggag ctggacaccg
     1381 gacggaagtt ctggtggggt gactggaacc gcatgatccg cgacgtcatc acagcgcaac
     1441 gcacgggcga gccggtcctc tacgacgacg acgagtgaca ccgcgcgatc tgtctgaaag
     1501 ccccacttgt ctgcggcaga cagatcggcg ttctcggcag aacttctaac ggaacctcta
     1561 acggaccagg tgacacaggg tgttatccac agccatcagg tgacatgcca gtatcgcgaa
     1621 agccctgcta gatggcacta aacggcacag ggcatcacag ggtaacacca ccgttttacg
     1681 gctcataacc cgaaggtcgc aggttcaaat cctgcccccg ccaccaagga aaagcaccag
     1741 ctgagaggcc ctgccgcttc gacggcaggg cctcttcggt gcgcgcgatc cggtacctcg
     1801 tacaacatca gtacaacgga ttgcacttcg ggccggaacc cggaggtcac ctctgcgcaa
     1861 gccacctggt gcacaggtat ctggagaccg tgacccacgt ggcgagggcg gtcgtgctga
     1921 cggcatcgcg ccggcgcgtg cacggcgtcg gcctcatcat cggcgcagtg ccatcgaccg
     1981 cagtcacggg ccggaagggg tcgaggccct cgtagctgac ggtgtcccaa cgccgtcgcg
     2041 cttggtgaga tccatgttcg gttcctcctc gagcggtagc ggggccggtc gttgacctgc
     2101 accggctccg tacggcgaga agcgctgtcc atctgaagcc tgacgatccc cgtacgtcat
     2161 cgcagaccga ctggcgaccg ctccctccgc tcggttcgat gagcgctcca gatgccggtg
     2221 cggtaccccg gcaggttgat ctcccgatac ggtcatgctg ggcacgatca ccgagaggag
     2281 tatcccatgg ccgcgccgat gcttcctgtg caactcgtcg aggctcggcg gactccgctg
     2341 ttcgacttcg cgacgttgcc cgacgcgctc gccggctcgc accgcaccac cgtgtgggcc
     2401 gagcttcgtg tgcaggccgg cagtgtacgg tacgtcgatc tcgagggtga tgatccgcga
     2461 gatgagcgac tcgtcgccgg tgagagcgtg gtgatcgtcc cggggatcga gcatcaggtc
     2521 gagccgtcga gcgacgcgca gttctacgta cagttcttcc gcgaacctga cgcacccatg
     2581 gtgcccggtg tcgagccccc ggccagtgtc gaccgttccg gtccgtggat gcatcgcggt
     2641 tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnngtc acgtcctgct atatgcccct ggctacgaca
     2821 tcgacgtgat cgaggttcac cgacaccttc acggcagcgc accgttcaca tctgagctgt
     2881 tcgaccgttg gctgcaggtg ttccacgaca ccgtcgccgg cggctgggtc gggccgaagg
     2941 cgaccatggc gaacaagcgg gccaccggga tggcgtgggc catggcacag cgcttcctcg
     3001 ggcacggcgt gtggcgccct gccgatcgtc gctagaggga cgcgcctgtg tcgacgctcg
     3061 acgacggtca gcggggcggt tcatcgagga ggtgggcgaa gcagggtgac tcgccgcctt
     3121 gatcgtggtg atcggcgatc ggcggtcgga tgacgtcggg agcgtcagcg ccggacgtgt
     3181 tcgtgtcgcg agcgagcttc gcgaggcgcg ccagcgcgcc gaggcctcgg gagacgtagc
     3241 cgtcggggag caccatcact ggcacgatcg gatcggtgag gtgctcccac tcctcgcgcc
     3301 accgggcggc tgttgccgtg tggatggtcg tcgccccgag ccgtgccagg gtcgtgtcgt
     3361 cggtgagatc tgcaccttcg ctccagtggg ccgcgaacag gcggtcacgc actgctgggc
     3421 gatcggcgat gggtgtggca gcgaacgcgg cggtcgccag ccgggtgttg ggttgacggg
     3481 cgggaaggcg gagtcggtcg ggctcatcga cgctcaggag accccggatc tgcgcgagct
     3541 cgcgttggat ctcggacgcc agctcgccgg cgatctcgat gcccgcgggt gggaggtcgg
     3601 gggcgtgctc gaccgctcgc cagtcgacgc gaacggcacc gattcgttcg aggctgctga
     3661 cacgggcgct ggccagggcg ctgaagggac agttgaagtc gccgtacacg atcagctcga
     3721 cgggttgact ctccaacatc tcacctcatt cttctagccg tgaacggaag tataggagag
     3781 gtagtctgga tggtgtggtg cagcgatgac ggtgttcgtt tgtcgaccgc accccggtga
     3841 ggaccccaga tgaccacgtt cctgttgccg acggacccga tcgtctcgat cgacgactac
     3901 ctcgcgaacg gcgtcgccgg atctggcatc cgccgcgctc atgagctggg tccgaaggcc
     3961 acgatcgatc tggtgacgca gtcggggctg cgcggtcgcg gcggtggtgg cttcccgacc
     4021 ggtcagaagt gggccggcgt cgctgctcat acaggcggcc ggcgatacct ggtgtgcaac
     4081 ggtgccgagg gggagccggg cacgttcaag gaccgcgcga tgttgcgtgc gaatccgtat
     4141 cagctggtcg aggggttggc gatcgctgcg ttcgcgatcg gagcgacgga ggcgttcgtc
     4201 tgtctgaagg cgagcttccc gcgcgagatc gacgcggtca ctcgtgcggt gcaggagttt
     4261 cagtcagcag ggctgtgtgg tgactgcaag gtcacgatcg tggccggacc ggaggagtac
     4321 ctgttcggtg aggagaaggc gatgttggag gtgatcgagg gcaacgagcc gttgccgcga
     4381 tggcttcccc cgtacctgca cgggttgttc gcgaccgcac cgcaactggg ctggcagtcc
     4441 cacgacaccg aagccggaag gagcggcggc gggggtggtt cgaacccgac gctcgtcaac
     4501 aatgtcgaga cactctccac tgttgcgcac atcctggccc gtggggcgga gtggttccgg
     4561 tcgatgggta ccgtcgagtc gcccggcacc gtcgttgcga ccgtcgtcgg cgacgtggtc
     4621 gcccccgatg tcggcgaggt cgagatgggc acgtcgttgc gggacgtgat cgacgcggtc
     4681 gggagtgggc tggccgaggg acggtcggtc aaggcggtgt tctccggcgt cgcgaacgcc
     4741 gtcgtgaccg ctgcggatct cgatgttccg gtgagttacg aggggttcca ggcgatcggg
     4801 agcggcatgg ggtccgcggg gttcatcgtc tacgacgaca cgacgtgcat ggtcgacgcg
     4861 tcatatcgct tctcgcggtt cttgtcgatc gagtcatgcg gtcagtgtcc gccgtgcaag
     4921 ctgggctcga gcgcgatcac cgagcatctc gaacgcatcg agaccggcgt cggcgacaca
     4981 ggnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncaagc tgatcgacct
     5161 ggccgatggt cgggccgtct acgacgagtc gttctggcgc aagcagcccg attggaccta
     5221 cgccccgggc atcggggcag atgacggaac ggagcgccga tcgtgacgcc gtcacatccc
     5281 acgtggaagg cgaacctgtt gatggctggt gtcgtggtcg cgctgctggt gatctcggtg
     5341 gtgagcgtgg tgacgggccg atgagtgcgc tgcagaccca ggcccgagcg ctgggtgacc
     5401 cgacgcgcca cgagctgttc cgttacatcg tcgacgcagg tcgtccggtc gacgtggtcg
     5461 aactgaccga ccacctcgag ctccaccaca acgctatccg tcagcacctc gccaagctcg
     5521 tcgacgctgg actcgtggtg gaggcgaggg cgccacgggt cgggcgtggc aggccgcgcc
     5581 tcgtctacac ggtggaccct gcggccgaga gtcggtgggg agtcaccggc ccgtacgagc
     5641 gactgtcgct gctgttgtcg gagatcatcc ggaccgggga ctcacctgtc gaagttggcc
     5701 gccgagctgg tcggcaggtg ccggccgcag tgcgcgacac caccgatccg gtcgtcgggc
     5761 tggtcgacgc catggaacga cacgggttcg agccgaccgc caagcggcga gggaatcagg
     5821 tcgagatcgt gttgggcgcc tgcccgttcg ccaccacggc gttggctgac cccgagacgg
     5881 tgtgcgggct gcacctcggt ctcgcccacg gcgccgccga gcagctcggt ggcatcgtga
     5941 tcgacgaact caggccccgc gatccccgtc gcggggcctg ccgcctgcgc tgtcacgtcg
     6001 cgacatgacc ggcccgatgg atacgctcgc cgaccggatg cgccagctgg aggcggcggg
     6061 ttgggccaag cagctctccg tcagcgatgc cgggctgcgc tgcgacggct gtcgatgctg
     6121 ggccgccccg gaggacgtcg tggtcgacgt tgtgtaccgc ttcgaggggc ccnnnnnnnn
     6181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cgttgtcgac gtcgtgaagc gactgcgatt
     6301 cactcgggac tgagccgccg atccccgccg gctgtattta tctagtggac aatagaagta
     6361 ttggtgggat cggagtaggg tggacgatga ggaggggcgc gacccccgtc aaatcggagg
     6421 cctccacctc gctgagatga aagaaggcga ccatgcagac gtcggttcgg atcgacgggg
     6481 cgagctgtcc cacgtgtttc aacgagacca tcgacgcgct ctcccagctc gacggtgtac
     6541 gccgcgtcca cggttcgttc gccggcccat gcctcgagat cgaccacgac attccgctgg
     6601 aaacgatcaa cagcaccatc cgcggccgct tgcacggtgt cgagatcttc gcgaacgaga
     6661 tccgcatggt gccgctcgaa cccgccccgc tgaccacgac gtgcgtccac catcagcccg
     6721 aagtggcgga cccgacggca ccgccggact caggtgggaa cactgtcgat ccgtcgatga
     6781 cgctcggtga gatcgtcacc cgccgacccg tactggctgc cgagctggag cggcgcggac
     6841 tcgactactg ctgccacggc gaccgcacgc tcaccgatgc agcgctcgag gcgggcttgg
     6901 acgctcggac cgtcgccgac gagttgtcgg cggtcgcagt cgacgctcct gcagctgcgt
     6961 gggcgtcgct cggtccgtcc gagctggtcg aacacatcga cgccgtccac catcgatatc
     7021 tgtgggccga acttccgcgg gtcactgcac tggtcgacaa gatcgccagc gtccacggcg
     7081 aacgccaccc cgaactgttc gaggtgcaac ggctctatgg tgagttgcga gccgacctcg
     7141 agccacacct caccagggaa gagcaggagc tgttcccacc catcagacag ctggccgggg
     7201 ccagcgaccc gtcctccgtc tcgacccgag ccttcgcggc gcagatcgaa acgttggccg
     7261 atgagcacga gacggtcggt gcgctcctcg aggagctgcg tcgcgtgacg tccggctact
     7321 cggttcctcc ggacggttgt gcaagctacg ccgcctgcta tcgggccctc gccgacctgg
     7381 aggccgacac ccatctccac gtgcacaagg agaacaacct gctcttcccg gcggtcctca
     7441 agacgcgtct cggcgtcgac gaggtgcgcc agcacgggca ggccccattc gcgcagcgtc
     7501 aggaacaaga gcgccgcgg
//
LOCUS       DAORNF010000053         7442 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_7364_length_7442_cov_1.926086, whole genome shotgun sequence.
ACCESSION   DAORNF010000053 DAORNF010000000
VERSION     DAORNF010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 7442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7442
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7364_length_7442_cov_1.926086"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..957)
                     /locus_tag="PLV68_03360"
     CDS             complement(<1..957)
                     /locus_tag="PLV68_03360"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HRE00308.1"
                     /translation="MRRRLGRMAALALIAAALPITSPISALAAGAGVVINELYYNPAD
                     GNQAEEFVELFNDGDTPVDLSDWYFSGITLTFPAAFTIEPGAFVVLRPAADAVHPAAL
                     RYSGALSNSGERIRLRNAAGTTIDDITYDDHGEWPAMADGEGDSLQRRDPSVVGTKPG
                     NWVSAPPTPGAANALTGGLMPTFAAVGHTALPQPGEPITVTATVADAASVSLTYRVGF
                     DSEVTVPVTIDGSAASGTIAATIPGQDAGALVRYRLDAVSPDGVPGTWPRQGDGSGYW
                     GTTVAQPAASALPTLQWFMPDDVYHQAYDDITLSGDDGYPAVV"
     gene            1052..>1539
                     /locus_tag="PLV68_03365"
     CDS             1052..>1539
                     /locus_tag="PLV68_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00309.1"
                     /translation="MRAWVRAAIGLVVALVGVMLAASLATVGAQAGDGQAAPAKVVAA
                     VGDRERLDVVGDSLTARARSEHADAFAAVGWTSVAVDASSQRTIDMPTADDHRSGLDA
                     VGRLQATHDASLWVVALGTNDARWNPPARYRDLIVAMLDRIGPGHLVVWVNVYLPAKP
                     AL"
     assembly_gap    1540..1839
                     /estimated_length=300
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1840..2922
                     /locus_tag="PLV68_03370"
     CDS             <1840..2922
                     /locus_tag="PLV68_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00310.1"
                     /translation="GPAGGGQPASGGSTVRIAMDDLAPANTTAVALNLTITDAAAAGY
                     ATAWSCDRSRPSTSTTNFGTGNVGAGSPTASFSFTAVGASRSVCVFVSATAHVVVDVF
                     AAFVAGSGAERGLGWRAVGPVRVADTRAGAAAMAAGTVVEVAIPIDRGAGRSGAAVFV
                     NLTVADAATGGYLTAWSCDQARPATSNVNYTGQRSSAGAGQVPIANAAVVSIGPSGRL
                     CVFASSPAHVVVDLFGVFDAAGTGSFQPAVPTRILDTRDGTGGWAGSLMSARAIDVAT
                     ALASGTTVVATVTATDVFGSGFVTASTGGVAGRPFVSNLNVVPGSTRPNLAVVALGPD
                     ASVRLSVGGNVATTMIVDVTGWFTPD"
     gene            complement(2945..>3291)
                     /locus_tag="PLV68_03375"
     CDS             complement(2945..>3291)
                     /locus_tag="PLV68_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00311.1"
                     /translation="DTRRGPKPADRSQTVITGLPNRSAVVSLVATETTGPGYVQVLPC
                     GDAPGAASNINADHAGQTVSGLAVIRFGADGQACIFTQTATHLVVDLQSYLADAAFDD
                     IPDQRLVDTRNR"
     assembly_gap    3292..3405
                     /estimated_length=114
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3406..>4472)
                     /locus_tag="PLV68_03380"
     CDS             complement(<3406..>4472)
                     /locus_tag="PLV68_03380"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00312.1"
                     /translation="NALVKRRIRTLTEQLLNPDVVAARVAELTDLIEADAAADRAVWH
                     TYGSQQTARYAAGQIISNYVVPQYQRLLGSFANSGRIARWPQPEPPAVSMAVDHATGI
                     VTITNHSGDSVDISQFRIDGIGLVVPGGTVLLPGRSARYFSDSAASLVGLFGAAPGGV
                     YTGSLADNKPLELVTRAGTMVARDARLAPGKTTTIKGSPNRSAVISIIATETGAPGYL
                     QVLACGTAPGATSNLNADRAGQTVAGLAVIRFDAKGEACVYNQSVTHVVVDLQGYLAT
                     AAFDDIADVRLVDTRNGPRPEAGTQVTITATPNRSAVVSLISVDNAAPGYLQVLPCGD
                     APGATSNLNTSRAGQIAAGLA"
     assembly_gap    4473..4483
                     /estimated_length=11
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4484..6194)
                     /locus_tag="PLV68_03385"
     CDS             complement(<4484..6194)
                     /locus_tag="PLV68_03385"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HRE00313.1"
                     /translation="MLRRCAALVTFLLIAALVPTALIAPTPAVAAGAGVVINELYYNP
                     VDGNQANEFVELLNASDTAVDIGGWYFSGITFTFPAGFVVAPGAFVVLRPGLYSGALS
                     NSGERIRLRDASGKTIDEVEYDDNGEWPSMADGEGSSLERRVPGVSANKPGNWVSGGP
                     TPGAPNSNLGGVLPSFSGVTHTVLPAPGQPIEVTAKVTDAVSAALVYRVGFGVEVPVP
                     VTLSGTTLTATIPGQASGQLVRYRITATSAEGVIGAWPRQGDGAGYTGTTVAAPSASP
                     LPTLQWFMEDAVYNAAFKDLTLSGDDGYPAVFAYAGTIFDNSKIRVKGQVSRTQPKKK
                     WKVIFAKGYALDIPGVLPEPVDEFALHSSYSDKSFIRETLASEMLTAAGIPVSQSFPM
                     RIERNGAFYGLYSYVEQQDGTWRDRAGYDDSIVYEVGGGKQFALLDASDANLGQTQLR
                     NKYEKETQEWLGDDELRTLIRTLNGLSGQARRDWIYANVDVASVVNLIAGVVVIQHQD
                     LGHKNYRLIFNEHQRWEVVPTDFDLVLGRRWSVDTGAVSDVVGIQGAFEHPGGPLFGA
                     FWFD"
     gene            complement(6233..6841)
                     /locus_tag="PLV68_03390"
     CDS             complement(6233..6841)
                     /locus_tag="PLV68_03390"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRE00314.1"
                     /translation="MSTRLTAAARREQLLDVALNVFARQGYHGASMNDIAEAAGVTKP
                     VVYQHFDSKRELYLALLEAVGERLITAIAKATASATNGKQQTELGFQAYFRWVASDHD
                     AFLLLFGSGARRDEEFNDAVRAVTAQVADAIKPLIAADIEPEHQTTLAHGLVGLSEGV
                     SRRLVDLGLDFDPDLLAQQVSDLAWAGLRAIGRPGATRVRRG"
     gene            complement(6863..7165)
                     /locus_tag="PLV68_03395"
     CDS             complement(6863..7165)
                     /locus_tag="PLV68_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00315.1"
                     /translation="MATDSPAPHSAAPDRNGPPDPVRARRARVAKWNGIVTRVGYALF
                     GIAMVLFFVALATGFNSTMGTLIAACLIVGSILLAPAIVIGYAVKAAEKDDVARGL"
     gene            7198..>7442
                     /locus_tag="PLV68_03400"
     CDS             7198..>7442
                     /locus_tag="PLV68_03400"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRE00316.1"
                     /translation="MTVHVEFSDRVAVVTIDRPQRRNAVDHETLLALRAAQLEVAERG
                     ARCLVLTGAAPAFCAGADLKAVAAGRGLKLWNTVSSTT"
BASE COUNT         1032 a         2281 c         2578 g         1126 t
ORIGIN      
        1 gacgacggcg gggtagccgt cgtcgccgct gagcgtgatg tcgtcgtatg cctggtgata
       61 gacgtcgtcg ggcatgaacc actgcagcgt cggcaacgcc gacgccgctg gttgggcgac
      121 ggtcgtgccc cagtagcccg aaccgtcccc ctggcgtggc caggtgcccg gaactccgtc
      181 gggggagacc gcgtcgagcc gatagcgcac cagcgcgccc gcgtcttgtc ccgggatcgt
      241 cgccgcgatc gtgccagagg ccgccgatcc gtcgatcgtg accggcacgg tgacttcgct
      301 gtcgaagccg acccggtacg tcagcgagac cgacgccgcg tcggccacgg tggcggtcac
      361 ggtgatcggt tcgccgggct gtggcaaggc ggtgtggccg acagcggcga aggtcggcat
      421 caatccgccg gtcagcgcgt tggccgcacc gggagtgggc ggggccgaga cccagttgcc
      481 cggcttggtg ccgacgacgg acggatcacg ccgctgcagc gagtcgccct caccgtcggc
      541 catcgccggc cactcaccat ggtcgtcata ggtgatgtcg tcgatggtgg tcccggccgc
      601 gttgcgcagc cgaatgcgct ccccgctgtt gctgagcgcg ccgctgtagc gcagcgcggc
      661 cgggtgaacg gcgtcggctg ctggacgcaa caccacgaac gctccgggct cgatcgtgaa
      721 cgcggccggg aaggtgagcg tgatgccgct gaagtaccag tcgctcaggt cgaccggtgt
      781 gtcaccgtcg ttgaagagct cgacgaactc ttcggcctga ttgccatcgg ccgggttgta
      841 gtacaactcg ttgatcacca cccccgctcc tgcggcgaga gcggagatgg gactcgtgat
      901 cggcagtgca gctgcgatca gcgcgagagc ggccatgcgg ccgaggcgtc gacgcattcg
      961 gtccaaccta gcaacgacgt cgggcgacgg cccagccacc gaccgtgagc agcgggttcg
     1021 gccacgagat gtgggcggta agaatgggcc ggtgcgggcg tgggtgaggg cggcgatcgg
     1081 gctggtggta gcgctggtcg gagtgatgct ggccgcgagc ttggcgacgg tgggcgcgca
     1141 ggcgggcgac gggcaagctg cgccagccaa ggtggtggcc gcggtggggg atcgagagcg
     1201 actcgacgtg gtgggggatt cgttgaccgc gagggcgcgg agtgagcatg cggacgcgtt
     1261 cgccgcggtg gggtggacgt cggtcgcggt cgacgcgtcg tcgcagcgga ccatcgacat
     1321 gccgaccgcg gacgatcacc gttccggtct cgacgcggtt ggccgcttgc aggccacgca
     1381 cgacgcgtcg ttgtgggtgg ttgcgctcgg caccaacgat gcccgttgga accctccggc
     1441 ccgctaccgc gatctgatcg tggcgatgct cgaccgcatc ggccccggtc atctcgtggt
     1501 gtgggtcaac gtgtatttgc ccgccaagcc tgcgctggan nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng gccccgccgg tggcggccag
     1861 cccgcatctg gcggcagcac cgtgcgcatc gcgatggacg atctggcgcc cgcgaacacc
     1921 acggcggtcg cgctcaacct caccatcacc gacgcggcgg cagcgggcta cgccacggcg
     1981 tggtcgtgcg atcgcagccg accctcgacc tcgaccacga acttcgggac tggcaacgtt
     2041 ggggcgggct cgccgaccgc gtcgttcagc ttcaccgcgg tcggggcgtc gcgatcggtg
     2101 tgcgtgttcg tcagcgccac ggcgcacgta gtggtcgacg tgttcgccgc gttcgtcgcc
     2161 gggtccggag ccgagcgcgg attgggctgg cgtgcggtcg gtcccgtgcg cgtggccgac
     2221 acgcgtgccg gtgctgccgc gatggccgcg ggcaccgtcg tcgaggtcgc aatcccgatc
     2281 gacaggggtg ccggacgatc tggcgccgcg gtgttcgtca acctgacggt ggccgacgcg
     2341 gcgaccggcg gctacctcac cgcgtggagt tgtgaccaag cgaggcccgc gacgtcgaac
     2401 gtcaactaca ccggccagcg ttccagcgcc ggcgcggggc aggtgccgat cgccaacgcg
     2461 gcggtggtgt cgatcgggcc gtccgggcgg ctgtgcgtgt tcgcgtcgtc gccggcacac
     2521 gtcgtggtcg acctgttcgg cgtgttcgac gcggccggca cgggcagctt ccaacctgca
     2581 gtgcccaccc gcatcctcga cacgcgcgac ggtaccggcg gatgggctgg gtcgctgatg
     2641 tcggctcgcg cgatcgacgt cgcgaccgcg ctcgcgtcag gcacgaccgt ggtcgcgacg
     2701 gtgacggcga ccgacgtgtt cggcagtggc ttcgtcacgg catcgaccgg tggtgtcgcc
     2761 ggtcgtccgt tcgtgtcgaa cctcaacgtg gtgccgggga gcacccggcc caacctggcg
     2821 gtggtggcgc tcggccccga tgcgtcggtg cggctgtcgg tcgggggcaa cgtcgccacg
     2881 acgatgatcg tcgacgtcac cggatggttc acgcccgact gaatgcgtcc gcccagatcg
     2941 cggatcagcg gttgcgggtg tcgaccaggc gctgatctgg gatgtcgtcg aaggccgcgt
     3001 cggcgaggta gctctgcagg tcgaccacca gatgggtggc ggtctgggtg aagatgcagg
     3061 cctgcccgtc ggcaccgaag cggatcacgg ccaaccccga cacggtctgg cccgcgtggt
     3121 cggcgttgat gttcgaggcc gcgccgggcg cgtcaccgca gggcagcacc tggacgtacc
     3181 cgggtccggt cgtctcggtc gccaccagcg acacgacggc cgagcggttg ggcaggccgg
     3241 tgatcacggt ctggctgcga tcggctggct tcggtccgcg gcgggtgtcg annnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnatggc caggcctgcc
     3421 gcgatctgcc cggcccggct ggtgttgagg ttgctggtcg cgccgggcgc gtctccgcac
     3481 ggcagcacct gcagatagcc gggggcagcg ttgtcgaccg agatcagcga caccacggcc
     3541 gagcggttgg gtgtcgcggt gatggtcacc tgcgtccccg cctccgggcg cggcccgttg
     3601 cgcgtgtcga ccaggcgcac gtcggcgatg tcgtcgaacg ccgcggtcgc gaggtagccc
     3661 tgcaggtcga ccaccacgtg ggtcaccgac tggttgtaga cgcaggcttc gcccttggcg
     3721 tcgaagcgga tcaccgccaa cccggccacg gtctgaccgg cgcgatcggc gttgaggttg
     3781 ctggtcgcgc ccggcgcggt gccgcacgcc agcacctgga gatagccggg tgcgccggtc
     3841 tcggtggcga tgatcgagat gaccgcagag cggttcggcg atcccttgat cgtcgtcgtc
     3901 ttgcccggtg ccaggcgagc gtcgcgggcc accatggtgc ccgcccgcgt caccagttcc
     3961 agtggcttgt tgtcggccaa cgagccggtg tacacaccac cgggcgcggc gccgaacagg
     4021 ccgacgagcg atgctgcact gtcgctgaag tagcgagccg accggcccgg aagcagcacc
     4081 gtcccgccgg gaacgaccag gccgatgccg tcgattcgga actggctgat gtcgaccgag
     4141 tcaccgctgt ggttggtgat ggtgacgatg ccggttgcgt ggtcgaccgc catcgacacc
     4201 gccgggggct cgggctgggg ccaccgggcg atgcggccgg agttggcgaa cgagccgagc
     4261 agccgttggt actgcggcac gacgtagttg gagatgatct gacccgcggc gtagcgagcg
     4321 gtctgttgcg acccgtaggt gtgccacaca gcgcgatcgg ctgcggcgtc ggcttcgatc
     4381 aggtcggtga gctctgccac ccgggcggcg acgacgtcgg ggttgagcag ctgctcggtc
     4441 agcgtgcgga tgcgtcgctt gaccagcgcg ttnnnnnnnn nnnggtcgaa ccagaacgct
     4501 ccgaacagcg ggccaccggg gtgttcgaac gcaccctgga tgccgacgac atcgctgacc
     4561 gcgccggtgt cgaccgacca gcggcgcccg agcaccaggt cgaagtcggt cggcacgacc
     4621 tcccaccgct ggtgctcgtt gaagatcaag cggtagttct tgtgccccag gtcttggtgc
     4681 tggatcacga cgacgccggc gatcaggttg accaccgagg cgacgtcgac gttggcatag
     4741 atccagtcgc gtcgggcctg cccgctcaat ccgttgagcg tgcggatcag cgtgcgcagc
     4801 tcgtcgtcgc cgagccactc ctgggtctcc ttttcgtact tgttgcggag ttgcgtctgg
     4861 ccgaggttgg cgtcgctggc gtcgagcagc gcgaactgct tgccgccgcc gacctcgtac
     4921 acaatcgagt cgtcgtaccc cgcccggtca cgccaggtgc catcctgctg ttcgacgtag
     4981 gagtacaggc cgtagaaggc gccgttgcgc tcgatccgca tcgggaagct ctgcgacacg
     5041 ggaatgcccg cagccgtcag catctccgag gccaacgtct cgcggatgaa cgacttgtcg
     5101 ctgtacgacg agtgcagcgc gaactcgtcg accggctcgg gcagcacgcc cggaatgtcg
     5161 agcgcgtagc ccttggcgaa gatgaccttc cacttctttt tcggctgggt gcgcgacacc
     5221 tggcccttca cacggatctt ggagttgtcg aagatcgtgc ccgcgtaggc gaacaccgcc
     5281 ggatagccgt cgtcgcccga cagggtcaga tccttgaacg ccgcgttgta gaccgcgtcc
     5341 tccatgaacc actgcaacgt gggcagtggc gaggccgacg gcgcagcgac ggtggtgccg
     5401 gtgtagcccg cgccgtcacc ctggcgcggc caggcgccga tgacgccttc ggccgacgtc
     5461 gcggtgatgc ggtagcgcac gagttgcccg ctggcttggc cggggatggt ggccgttagg
     5521 gtggtgccgc tgagcgtgac cggcacgggg acctcgacgc cgaacccgac gcggtagacc
     5581 agcgcggccg agacggcatc ggtcaccttg gccgtcacct cgatcggctg gccaggagcg
     5641 ggcagcaccg tgtgggtcac gcccgagaac gaggggagta ccccaccgag gttgctgttg
     5701 ggtgcgccgg gtgttggacc gcccgacacc cagttgccgg gcttgttggc ggatacgcca
     5761 ggcacgcgcc gttccagcga ggatccttcg ccgtcggcca tcgacggcca ctcaccgttg
     5821 tcgtcgtact cgacctcgtc gatcgtcttg ccgctcgcgt cgcgcaggcg gatgcgttcg
     5881 ccgctgttgc tcaacgcgcc gctgtacagg ccggggcgca gcaccacgaa cgcgccgggc
     5941 gcgacaacga atccagccgg aaaggtgaag gtgatgccgc tgaagtacca cccgccgatg
     6001 tcgacggcgg tgtcgctcgc gttgagcagt tcgacgaatt cgttggcctg gttgccatcg
     6061 accgggttgt aatacagctc gttgatcacc accccggctc cggctgccac ggcgggggtg
     6121 ggagcgatca gcgctgtcgg caccagcgcc gcgatgagca ggaacgtgac gagggcagca
     6181 caacgtcgga gcatgaccag accgtagttg ctggcgccgc gagtgggtgc ggtcaacctc
     6241 gccggacgcg ggtggcgccg gggcggccga tggcgcgcag cccggcccag gccagatcgc
     6301 tgacctgttg ggcgagtagg tcggggtcga agtcgagccc caggtcgacc aagcggcgac
     6361 tgaccccttc cgacagaccg accaggccgt gggccagggt ggtctgatgc tcgggttcga
     6421 tgtcggccgc gatcagcggt ttgatcgcgt ccgcgacctg ggcggtcacg gcccgcactg
     6481 cgtcgttgaa ctcctcgtca cggcgggcac cgctgccgaa cagcagcagg aacgcgtcgt
     6541 ggtcgctggc cacccagcgg aagtaggcct ggaagcccaa ctcggtctgc tgcttgccgt
     6601 tggtcgccga tgcggtcgcc ttggcgatcg cggtgatcag ccgttcaccg accgcctcca
     6661 acaaagcgag gtacagctcg cgcttggagt cgaagtgctg atacaccacc ggcttggtga
     6721 ccccggccgc ctcggcgatg tcgttcatcg acgccccgtg gtagccctgg cgggcgaaca
     6781 cgttgagcgc gacgtcgagc aattgctccc ggcgggcagc ggcggtcaga cgggtcgaca
     6841 tggttaccgt agggtaacaa gttcagaggc cacgggccac gtcgtctttc tcggccgcct
     6901 tgacggcgta gccgatgacg atcgccggcg cgagcaggat cgacccgacg atcaggcagg
     6961 cggcgatcag cgtgcccatc gtggagttga acccggtcgc caaagcgacg aagaacagca
     7021 ccatggcgat cccgaacaac gcgtagccga cgcgggtgac gatgccgttc cacttggcga
     7081 cgcgggcgcg gcgagcacgc accgggtcgg gcgggccgtt tcgatcgggc gctgcggagt
     7141 gtggagcggg ggagtcggtc gccataaggt cactgcgatg gtagaggggg atcggcagtg
     7201 accgtgcacg tggaattcag cgatcgggtc gcggtggtga ccatcgatcg gccgcagcgg
     7261 cgcaacgcgg tcgaccacga gacgctgctc gcgctgcgcg cggcacaact cgaggtggcc
     7321 gagcgcgggg cgcgatgcct ggtgctgacc ggtgccgcac cggcgttctg cgccggggcc
     7381 gatctcaagg cggtcgccgc cgggcgcggg ttgaaactct ggaacaccgt cagcagcacc
     7441 ac
//
LOCUS       DAORNF010000054         7414 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_7387_length_7414_cov_1.560674, whole genome shotgun sequence.
ACCESSION   DAORNF010000054 DAORNF010000000
VERSION     DAORNF010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 7414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7414
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7387_length_7414_cov_1.560674"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="PLV68_03405"
     CDS             <1..235
                     /locus_tag="PLV68_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00317.1"
                     /translation="SDRYSEQDPADVSEALLAAAENLAERFDGVDGDQWLRTGTRSDG
                     ARFTVRTFAQYLIHDPIHHLHDVHLGNRMLAAG"
     gene            253..1434
                     /locus_tag="PLV68_03410"
     CDS             253..1434
                     /locus_tag="PLV68_03410"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HRE00318.1"
                     /translation="MTRTTRTSTSLRTPTRRPVRLARAVRLTAAAAIASAALVACGSD
                     GDSGATTTPTVATTTTVAPTSTTAAPTTTLPPTTTTAAATTTAPTTTVEATSTTVVVA
                     KPLTVDELLTLGRPIVLAHAGGEDQYPHSIPFAYGESVAAGVDMIDLDVQLTGDGVLI
                     VQHDPNTARTTEADLVVGESSFEQLHALDNAYWFTADCVCTDQPEDAYIYRGIRTGDK
                     PPPAGYTADDFAITPFSEIAARFPDKPVNIEIKGSGEAAVAAAKQLAIELEALGLLAS
                     AVVASFDDDVVAAFHDLAPSVEISPGLGASSAWVLDGVELPFGMRILQLPPVYGDIEV
                     LTPEVMARVAEAGYAVWVWPNDRDLENLASYLDLLERGMAGLNINFPALGVEAVAEFT
                     G"
     assembly_gap    1502..1529
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1530..>1858)
                     /locus_tag="PLV68_03415"
     CDS             complement(<1530..>1858)
                     /locus_tag="PLV68_03415"
                     /EC_number="1.14.19.2"
                     /inference="COORDINATES: protein motif:HMM:NF015370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045300 - acyl-[acyl-carrier-protein]
                     desaturase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-ACP desaturase"
                     /protein_id="HRE00319.1"
                     /translation="YEVMSRVANDENLHYLFYRDLATAAIEVDPSAMVIAIEQVVRNF
                     TMPGTGIVDFDRHSAVIADAGIYNVAILHEQVMVPVIHNHWRIPQLRGLSDAAERARD
                     KLMKRID"
     assembly_gap    1859..2049
                     /estimated_length=191
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2050..2377)
                     /locus_tag="PLV68_03420"
     CDS             complement(<2050..2377)
                     /locus_tag="PLV68_03420"
                     /EC_number="1.14.19.2"
                     /inference="COORDINATES: protein motif:HMM:NF015370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045300 - acyl-[acyl-carrier-protein]
                     desaturase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP desaturase"
                     /protein_id="HRE00320.1"
                     /translation="MNELEPVAAQLLDRHLSVAKEWFAHELIPYGRGRDFERGYEWSE
                     DDTDLGGTDLAPAVRSALYVNLLTEDNLPYYFRDIERIFSTDGAFGTWSRRWTAEEGR
                     HSIAMRD"
     assembly_gap    2378..2432
                     /estimated_length=55
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2480..2896)
                     /locus_tag="PLV68_03425"
     CDS             complement(2480..2896)
                     /locus_tag="PLV68_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HRE00321.1"
                     /translation="MAPGKDPTAETRAEAALQHVRAAALALPQGTEKLSHGAPSFFIG
                     DKKCFTMLHDDHHGDGRLAIWCPAPDGAQAEMVDEDPERFFVPPYVGWRGWLGVRLDV
                     RPDWDEVAAIVTDAYRCVAPKRLLAELDQQHRERPR"
     gene            complement(2896..3195)
                     /locus_tag="PLV68_03430"
     CDS             complement(2896..3195)
                     /locus_tag="PLV68_03430"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HRE00322.1"
                     /translation="MPRSSSATPSAWREGQTDIAIGVAGIHPIRSYIGQLDPHGHEFR
                     VQALCVADEIAGAAELVKGNVSRIPLAVVRGYDWQPDDAATIAPVLRDQSKDLFR"
     assembly_gap    3514..3569
                     /estimated_length=56
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3841..3958
                     /estimated_length=118
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3959..4647
                     /locus_tag="PLV68_03435"
     CDS             <3959..4647
                     /locus_tag="PLV68_03435"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HRE00323.1"
                     /translation="PTDGGWAFCSGGDQRIRGRDGYKYTTADDDGSLGETAASIEPGR
                     AGRLHILEVQRLIRFMPKVVICVVPGWAAGGGHSLHAVSDLTLASREHARFKQTDADV
                     ASFDGGFGSAYLARQVGQKFAREIFFLGQEYSAEQMHQMGAVNAVVDHADLEATALEW
                     GRLINGKSPTAQRMLKYSFNLIDDGLVGQQLFAGEATRLAYGTDEAQEGRMSFLDKRD
                     PDWSPYPWLF"
     gene            complement(4674..4856)
                     /locus_tag="PLV68_03440"
     CDS             complement(4674..4856)
                     /locus_tag="PLV68_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00324.1"
                     /translation="MCNDRIETGDVLEMTLEDELISALVLLATDTAVILDTCDGSTPF
                     VVRREDLVDYRLFSPN"
     assembly_gap    5108..5305
                     /estimated_length=198
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5306..6016
                     /locus_tag="PLV68_03445"
     CDS             <5306..6016
                     /locus_tag="PLV68_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRE00325.1"
                     /translation="RAVIVTGAGRGFCAGADLSSGGSTFAHGGSDIQTRVGVPRDGGG
                     MVSLRIFASKKPVIGAINGPAVGVGATMTLPMDIRLCSDSARFGFVFSRRGLVPEACS
                     SWFLPRLVGISQATEWVYSGRVFDADEALAGRLVRSVHAADELLPTARAIATEIAANT
                     APVSVALSRQMLWRMLGASHPMEAHRVESRGIQSRGASADAFEGVTSFLEKRPAAYPD
                     TVSAGLPDIFPDYTEPEF"
     gene            complement(6061..>6721)
                     /locus_tag="PLV68_03450"
     CDS             complement(6061..>6721)
                     /locus_tag="PLV68_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00326.1"
                     /translation="VSTVVDVDKLQYYDFAKSYEEAQRLLAGDPGTLKLLDEAHLPTM
                     FKIAPLSGKTDELVLYADAFRDLPAVHSVALAKDQIEQIGKLSDFVQTVTVGLSVALL
                     VGAVILIWTTIRTAMFARRREIEVMKLVGATNWFIRIPFMLEGLIQGLVGGLVSCGLL
                     VTFNRLWTNGVVTLEGDNGFAALVVSDGYLNGVMLGVVAFGMIAGAISAGVAATRFLD
                     V"
     assembly_gap    6722..6851
                     /estimated_length=130
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7088..>7414)
                     /locus_tag="PLV68_03455"
     CDS             complement(7088..>7414)
                     /locus_tag="PLV68_03455"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRE00327.1"
                     /translation="RNIGNIFQDYKLLLNKTVHENVAFALEVIGRPKHVIRQQVPAVM
                     ELVGLAGKQERFPHQLSGGEQQRLAIARALVHCWPTSPRATSTPARARASCGCSTGST
                     RRAPPW"
BASE COUNT         1098 a         2280 c         2185 g         1075 t
ORIGIN      
        1 cagcgatcgc tacagcgagc aagacccggc cgacgtctcc gaggcgctgc tcgcggcggc
       61 cgagaacctg gccgaacggt tcgacggcgt cgacggtgac cagtggctgc gtaccggcac
      121 gcgcagcgac ggtgcccgct tcaccgtgcg gacgtttgcg cagtacctga tccacgaccc
      181 gatccatcac ctgcacgacg tgcacctggg caaccgcatg ttggccgcgg gttgagcact
      241 accctcgcct ccgtgacgcg aacgaccagg accagcacct ccctgcgcac gccgacgaga
      301 cgacccgtca ggctggcacg ggctgtcagg ctcaccgctg cggcggcgat cgcatcggcc
      361 gcgctcgtgg cctgcggcag cgacggcgac tcgggcgcta ccaccacgcc cacggttgcg
      421 accacgacca cggtcgctcc gacgtcgacc acggcggccc ccaccacgac gttgccacca
      481 accaccacga cggccgccgc gacgaccacg gccccgacca cgacggtcga ggcaacgtcc
      541 acgaccgtcg tggtcgccaa gcccttgacc gtcgacgagt tgctgacgct gggccgcccg
      601 atcgtgctcg cccatgccgg tggcgaagat cagtatccgc actcgatccc gttcgcctac
      661 ggcgagagcg tcgcggccgg cgtcgacatg atcgacctcg acgtgcagtt gaccggcgac
      721 ggtgtgttga tcgtgcagca cgaccccaac acggcccgta ccaccgaagc agacctggtc
      781 gtgggcgaat cgagcttcga acaactccat gcgctcgaca acgcgtactg gttcaccgcc
      841 gactgcgtct gcacagacca gcccgaggac gcgtacatct accgcggcat ccgcaccggc
      901 gacaagccac cgcccgctgg ttacaccgcg gacgacttcg cgatcacgcc gttctccgag
      961 atcgcggctc gcttccccga caagccggtc aacatcgaga tcaagggcag cggtgaggcc
     1021 gcggtcgccg cggccaagca gttggcgatc gaactcgaag cactgggcct gctcgccagc
     1081 gcggtcgtcg catcgttcga cgacgacgtc gtggcggcgt tccatgacct cgccccctcc
     1141 gtcgagatct ctccggggct cggcgcatcg tcggcgtggg tgctcgacgg ggtcgagctt
     1201 ccgttcggca tgcgcatcct gcaactcccg ccggtctacg gcgacatcga ggtgctgaca
     1261 cccgaggtga tggcccgcgt ggccgaagcc gggtacgccg tgtgggtgtg gcccaacgac
     1321 cgcgacctcg agaacctggc cagctacctc gacctgctcg aacgaggcat ggccggactc
     1381 aacatcaact ttcccgcgct cggcgtcgaa gccgtcgccg agttcaccgg ctgagcgaca
     1441 cgggccgagc ggatccgact gggtcaggcg atcgccgctg cgaactgacg gcgttcggca
     1501 cnnnnnnnnn nnnnnnnnnn nnnnnnnnng atcgatgcgc ttcatcagct tgtcccgggc
     1561 ccgctcggcc gcatcgctga gcccgcgcag ttgcgggatc cgccagtggt tgtggatcac
     1621 cggcaccatc acctgctcgt gcaggatcgc gacgttgtag atgccggcgt cggcgatcac
     1681 agcgctgtgc cgatcgaagt cgacgatgcc ggtgccgggc atcgtgaagt tgcggacgac
     1741 ctgttcgatc gcgatcacca tcgccgacgg atcgacttcg atcgcggcgg tcgccaggtc
     1801 gcggtagaac aggtagtgca ggttctcgtc gttggcgaca cgcgacatca cctcgtacnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnna gtcgcgcatc gcgatcgaat ggcggccctc ttcggccgtc cagcgacggc
     2101 tccaagtgcc gaacgcgccg tcggtcgaga agatccgctc gatgtcgcgg aagtagtacg
     2161 gcaggttgtc ctcggtgagc aggttgacgt acagcgcact gcgcaccgcc ggggccaggt
     2221 cggtcccacc gaggtcggtg tcgtcctcgc tccattcgta accgcgctcg aagtcgcggc
     2281 ctcgcccgta gggaatcaac tcgtgcgcga accactcctt ggcaacgctg agatgccggt
     2341 ccaataactg ggcggcaacg ggctcaagct cgttcacnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncgaggtat ccggcccgat tcgtgacgat
     2461 cgtcacgcgc tcgacctgct cagcgcggtc gttcgcggtg ctgttggtcc agttcggcca
     2521 gcagtcgctt cggggcgacg cagcgatagg cgtcggtgac gatcgccgcg acctcgtccc
     2581 agtcggggcg cacgtcgagg cgcaccccca gccagccccg ccacccgacg tagggcggca
     2641 cgaagaaccg ctccggatct tcgtcgacca tctccgcctg ggcgccgtcc ggggccgggc
     2701 accagatcgc gaggcggccg tcaccgtggt ggtcgtcgtg gagcatggtg aagcacttct
     2761 tgtcaccgat aaagaacgac ggcgcaccgt ggctcagctt ctccgtaccc tgcggcagcg
     2821 ccagagccgc ggcgcggacg tgttgcagtg cggcctcggc ccgtgtttcg gcggtcgggt
     2881 ccttgcccgg tgccatcagc ggaacaggtc cttgctctgg tcgcgcagca ccggcgcgat
     2941 cgtggcggcg tcgtcgggct gccagtcgta gccgcgcacc acagcgagcg gaatgcggct
     3001 gacgttgccc ttgaccagct cggccgcccc cgcgatctcg tcggcgacgc acagggcttg
     3061 cacccggaac tcgtggccgt gtgggtcgag ctgaccgatg tagctgcgga tcgggtggat
     3121 gccggccaca ccgatcgcga tgtcggtctg accctcgcgc caggccgaag gtgtcgctga
     3181 tgatgaccgc ggcatcgacg ccagcggcca cgaacgtggc ccggatcgcc cgggccgacg
     3241 catcggaatc gtcgggcagc aacagcgccc gccctggcgc gccactgctc gactgatcga
     3301 cgcccgaatt ggcgcagacg aacccgtgcc aggtctcggt gatcaggatc ggacctgctt
     3361 ggcgcaccac ccgcttggcg gcgcgcagta cgacttcggt gaccgcgggg tccttgtccc
     3421 actgttcgct ccactggcgg gcgaacggcg acggctccac gtccgccagc tcgacgatgc
     3481 tgatctcgct cgatccgtaa gtgccaagtg gcannnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnng cagccgcatc gagagcgaga cggccgatgt
     3601 cagccccggg ttcgatttcg ggcaggccag ggatgccgat gatgtgcaag ccgccggagc
     3661 gcgcagggct ggtcatgccc gcagtcttac cgatgaccca ccgggcagga cacaatgggc
     3721 gggtgaccag cctccccaac gtctcgacca ccttcgatcc ggccgcctgg cagacggtgc
     3781 ccggtttcga cttcaccgac atcacctacc accgcgcccg ggcccacggc acggtgcgag
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnca
     3961 ccgacggacg gcggttgggc gttttgcagc ggcggcgacc agcgcatccg cggccgcgac
     4021 ggctacaagt acaccaccgc cgacgacgac gggtcactcg gcgagaccgc ggcgtcgatc
     4081 gaaccgggcc gcgctggacg gctgcacatc ctggaggtgc agcgcctgat ccggttcatg
     4141 cccaaggtcg tgatctgcgt cgtacccggc tgggctgctg gcggcggaca cagcctgcac
     4201 gcggtgagcg acctcacctt ggccagccgc gagcacgccc gcttcaagca gaccgacgcc
     4261 gacgtcgcca gcttcgacgg cgggttcggc tcggcctacc tcgcccgcca ggtcggccag
     4321 aagttcgccc gcgagatctt cttcctcggc caggagtaca gcgccgagca gatgcaccag
     4381 atgggcgcgg tcaacgccgt ggtcgaccac gccgacctcg aagcaacagc gctcgagtgg
     4441 ggccggctga tcaacggcaa gagcccgacc gcgcagcgga tgctgaagta ctcgttcaac
     4501 ctgatcgacg acggtctcgt cggccaacaa ctcttcgccg gcgaagccac tcgcctcgcc
     4561 tacggcaccg acgaggccca agaaggaagg atgtccttct tggacaagcg cgacccggac
     4621 tggtccccct acccctggct cttctgacca gcgacaggga tggccactga cgatcagttg
     4681 gggctgaaca ggcggtagtc gacgaggtct tcgcggcgga cgacgaacgg cgtcgagcca
     4741 tcgcaggtgt cgaggatgac ggccgtgtcg gtggccaaca acaccagggc cgagatcaac
     4801 tcgtcctcca gcgtcatctc caacacatcg cccgtctcga tgcggtcatt gcacaaggtt
     4861 gtcacggcat tgctccagtg gtgggtgtgg gacgaaccct cgtcccggtt gctgtcctgt
     4921 cggtgtagat ctcagcgtcg cttgatcgaa gcgtgacaac cacatcggct ccaaggaact
     4981 tccggcggac cggcgtgttt cccgtcacag ctatcggcgg ctgtggtgac gaccttgacg
     5041 aattcttggg acgatcttgg aagggtcttg agggaagcgg catcgctgcg ggggcgcgag
     5101 ccgtgccnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5281 nnnnnnnnnn nnnnnnnnnn nnnnncgggc cgtgatcgtc acgggcgccg gccgagggtt
     5341 ctgcgccggt gccgatctgt ccagcggggg ctcgaccttc gcccatggcg gcagtgacat
     5401 ccagaccagg gtaggcgtgc cgcgagacgg tggcggcatg gtctcgttgc ggatcttcgc
     5461 atcgaagaag ccggtgatcg gggcgatcaa cgggcccgcc gtcggcgtcg gtgccacgat
     5521 gacgttgccg atggacatcc ggctgtgctc tgactcggcc cgcttcgggt tcgtgttcag
     5581 ccgccgtggc ctcgtgcccg aggcctgctc gtcgtggttc ctgccgcgcc tggtcggcat
     5641 cagccaggcg acggagtggg tctactcggg ccgggtcttc gatgccgacg aggctctcgc
     5701 tggccggttg gtgcgcagcg tgcacgcggc cgacgaactg ttgcccaccg cccgcgcgat
     5761 cgccaccgag atcgccgcca acaccgcacc ggtcagcgtc gcactgtcgc gtcagatgct
     5821 gtggcgcatg ctcggcgcgt cccacccgat ggaggctcac cgggtcgagt cgcgcggcat
     5881 ccagtcgcgc ggtgccagcg ccgacgcgtt cgaaggcgtc accagcttct tggagaagcg
     5941 ccccgccgcc tatcccgaca cggtcagcgc cggtctcccc gacatcttcc ccgactacac
     6001 cgaacccgag ttctgagccc gagttcagag cccgagttca gagccgcaag cccgggggcg
     6061 ctacacgtcg aggaagcggg tggcggcaac gccggcagag atcgcgcccg cgatcatgcc
     6121 gaacgcgacc acaccgagca tcaccccgtt gaggtagccg tcgctgacca ccagcgcggc
     6181 aaagccgttg tcaccttcga gtgtgaccac gccgttggtc cacaagcggt tgaaggtgac
     6241 cagcaagccg cacgagacca gcccgccgac caaaccctgg atgagccctt cgagcatgaa
     6301 cgggatgcgg atgaaccagt tggtcgcgcc gaccagcttc atcacctcga tctcgcgtcg
     6361 tctcgcgaac atcgcggtgc ggatcgtggt ccagatcagg atcacggcac cgaccaacag
     6421 cgcgacggac aggccgaccg tcaccgtctg gacgaagtcg ctgagcttgc cgatctgttc
     6481 gatctggtcc ttggccaaag cgacggagtg cacggcgggc aggtcgcgga aggcatcggc
     6541 gtagagcacg agttcgtcgg tcttgcccga cagcggggcg atcttgaaca tcgtcggcag
     6601 gtgggcctcg tcgagcagct tgagcgtgcc cgggtcaccg gccaacagtc gctgggcctc
     6661 ttcgtaggac ttggcgaagt cgtagtactg cagcttgtcg acatcgacca ccgtcgacac
     6721 cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6841 nnnnnnnnnn ngctgcacca ggaacgtgcc accgaccaag aacagcgaca ccgccgaggt
     6901 gatgatggtg gccaccgtga gggtcacgtt gcggcgcagg ctggcccacg tctcgcggaa
     6961 cgtgtaaccg agacgggaca gcattactcg tacacccctc gggcttggtc gcgcacgatg
     7021 atgccgcggt ccatctggat gacgcgcttg cgcatcatgt tgacgatgcg ctggtcgtgc
     7081 gtggccatca ccacggtggt gcccgtcttg ttgatccggt cgagcagccg catgatgccc
     7141 tcgcccgtgc tggggtcgag gttgcccgtg ggctcgtcgg ccagcagtga accagtgccc
     7201 gagcgatagc cagccgttgt tgctcaccgc cggatagctg gtgcgggaag cgttcctgct
     7261 tgcccgccag gccgaccagt tccatcacgg ccgggacctg ctggcggatg acgtgcttgg
     7321 gacggccgat cacttccagc gcgaacgcga cgttctcgtg cacggtcttg ttcaacagca
     7381 gcttgtagtc ctggaagatg ttgccgatgt tgcg
//
LOCUS       DAORNF010000055         7347 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_7483_length_7347_cov_1.555129, whole genome shotgun sequence.
ACCESSION   DAORNF010000055 DAORNF010000000
VERSION     DAORNF010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 7347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7347
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7483_length_7347_cov_1.555129"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    313..391
                     /estimated_length=79
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    807..929
                     /estimated_length=123
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <930..>1323
                     /locus_tag="PLV68_03460"
     CDS             <930..>1323
                     /locus_tag="PLV68_03460"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376941.1"
                     /note="catalyzes the reduction of ribonucleotides to
                     deoxyribonucleotides; the rate-limiting step in dNTP
                     synthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HRE00328.1"
                     /translation="ETSLRQVIDRVVDTITTWGQEGGYFVDEAEAETFRAELKFILAT
                     QRAAFNSPVWFNIGVAGVPQQASACFILSVDDTMDGILNWYREEGTIFKGGSGSGINL
                     SKIRSSHELLQGGGTASGPVSFMRGADAS"
     assembly_gap    1324..1529
                     /estimated_length=206
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1530..>1846
                     /locus_tag="PLV68_03465"
     CDS             <1530..>1846
                     /locus_tag="PLV68_03465"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF014868.2"
                     /note="catalyzes the reduction of ribonucleotides to
                     deoxyribonucleotides; the rate-limiting step in dNTP
                     synthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HRE00329.1"
                     /translation="QAVVDDADWNLVAVTDGRVIRTVKARELWRQVATAAWECADPGL
                     QFDTTINTWHTAHATGRINGSNPCSEYMHLDNSACNLASVNLLKYLDDDGTFDIEAYK
                     ATI"
     assembly_gap    1847..1949
                     /estimated_length=103
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1950..>2388
                     /locus_tag="PLV68_03470"
     CDS             <1950..>2388
                     /locus_tag="PLV68_03470"
                     /inference="COORDINATES: protein motif:HMM:NF014868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00330.1"
                     /translation="NLGALLMALGLAYDSPAGRAWAAGLTSLMTGHAYATSARIASRM
                     GPFAGFAENESYMLNVLRMHRDASYRIEGADIVPTELLFAGQQAWEAAVVDGEEYGVR
                     NSQATVLAPTGTIGLMMDCDTTGIEPDLGLVKMKKLVGGGNMSI"
     assembly_gap    2389..2425
                     /estimated_length=37
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2426..3560
                     /locus_tag="PLV68_03475"
     CDS             <2426..3560
                     /locus_tag="PLV68_03475"
                     /inference="COORDINATES: protein motif:HMM:NF014868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase"
                     /protein_id="HRE00331.1"
                     /translation="ALRTLGYTPSQVDDIIAYIAEEKSILGAPHIAPEHIAVFACSMG
                     DNTIHYEGHVRMMGATQPFLSGAISKTVNMPEDATIEDVEALHLLSWRLGLKAVAIYR
                     DNCKVAQPLQTAKKGDATVDAEGRLVAATNGGAAPVVATQTVERIVERIVHQPVRQKL
                     PRSRRGRTFEFRVADCKGFATIGEYDNGQPGEIFLTVSKQGSTLSGIMDAFAKSISYG
                     LQYGVPLRAFVEAFTNMRFEPAGMTDDPDIRFASSIMDYLFRRLALEYLTYDERAELG
                     IFSVDERLQPTLPGVYESTIENRTGSEVAADPKSIPSAGELTVSLELGLAPEAPRSDN
                     TPAAGIPAARQSDSPMCMQCGVQMIRAGSCHACPSCGSTSGCS"
     assembly_gap    3751..3880
                     /estimated_length=130
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3881..4921
                     /locus_tag="PLV68_03480"
     CDS             <3881..4921
                     /locus_tag="PLV68_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00332.1"
                     /translation="RDATTEVSTTTAITTTTGAVPVTGELYIDTFEVLPDPATVPWLE
                     PVVSPPRLRLLAAERGYATTFVCPPNASCVPNVAKATLRYGADEVGSERRVLIEQYFG
                     EATADLEPQPNDTIGVRSVERGAGEVIRWREESGVLVVVSPLGGIASDEITAIVDSLQ
                     VGTTDVWPRWGIEAPAMLCVDAASRYAPLTPEGWQRFVLDVVPDGGCTGHTVLFMSLV
                     LPPTDDDAGALATFVFSADPTSSRTRPDDAETVEIDGITVTIDRSVRDVRGLPAVGVV
                     MPFGPVSLDAHGYLSPERLAALVRTVALVDATEWATLQAEAAAIREAAGTTTDTIDIP
                     QATDDVTVPGGG"
     assembly_gap    5280..5289
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5290..6975
                     /locus_tag="PLV68_03485"
     CDS             <5290..6975
                     /locus_tag="PLV68_03485"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HRE00333.1"
                     /translation="SGRGRIATMAAAELVTFHRRYAELIELMGRAGNYASLRFAADTS
                     VPANGALMARAQERSTAISTKLLFVELEWAAVSDEQAEAVLADPSPELDFIRHHLRSS
                     RRYIPYLLSEPEERVLTEKSVTGAGAWTRLFDELTAAIEVDVVQPDGTARTMPLMQGL
                     ALMQHPDRAVRQHAHAAVTEGLAPGLRTRAFVFNTLLNDKSIDDRLRNYPDWITSRNL
                     ANEASDQSVQALIDAVVSRYDIPQRWYALKATLLGVERLADYDRYASVAGTEREIPWS
                     VATEIVTDAYSSFSGELASIVQRFLREGWIDAPVTPGKRGGAFCAYTVPSHHPYVMLN
                     WTSRTRDVATLAHELGHGVHGYLSREQGVFQMSTPLTLAETASVFGETVTSKRLLASL
                     TDPGERLALLAATLEDSIATVFRQISMNRFEQAVHTARRAQGELSVDAFNELWVESQA
                     AMFGDSIEITEGYRTWWSYIPHFMHTPGYVYAYAYGQLLALSVYKRYEEVGDEFVPRY
                     LDLLRAGGSMTPEDLGRIVDCDLGDPQFWTGGLAIVDEQLVAAEQAAAAVARS"
BASE COUNT         1096 a         2274 c         2227 g         1062 t
ORIGIN      
        1 cctgccccac gggtggggac acggccaggt cggtacggcg acgcggatcg ccaacgagcg
       61 ggctggggtc aactcgaacg tcttgaccga cgagttggcg atggacccgc tgtcgggcac
      121 ctcggtgctg aatggcatcc cggtcgtggt ggaagcgctg gtcagcagct gaccggcgca
      181 ctgcggtggg ccgtcgtcca ccgttcgtcc accactcatc caccgctcgt caccgcttgt
      241 ccaccgttcg tccaccgctc ggcgccgctg gtacaccgga aattcacagg gttgttcccc
      301 tttcgatcca cannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngcactcgac ggcgacactg gcggcttggg
      421 gcaggaatcc cgaacctgcg acctcgcgcc gcggccacat catgcccgcc ttccgggcgg
      481 ggttcttgac agcagcgtaa ctacaatggt gtaatcgctc ttccgctacc gcttgtgttg
      541 cccgcgcggg tttcggggga acaagcacaa catcttgtgc cacggcatgt gccgtggcac
      601 tgggtcatcg gctccgatca cccacctcgt ctcgccatct ccaacgattc agcacgattt
      661 cagcaccaac gattcaagga acgtcccaat gtcacttgct cccgaacgac tgtcgatcgg
      721 tctccgccgg cacttcacca cgcccggtgt cgacccgtac gaccagctcc gctgggagcg
      781 ccgtgacgcg cgcatctcga actggannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc gagacgtcgc tgcgccaggt gatcgaccgc
      961 gtggtcgaca cgatcaccac atggggtcaa gagggcggct acttcgtcga cgaagcagag
     1021 gcggagacgt tccgggccga gctgaagttc atcctcgcca cgcagcgggc ggcgttcaac
     1081 agcccggtgt ggttcaacat cggtgtcgcc ggtgtgccgc agcaggccag cgcctgcttc
     1141 atcctgtcgg tcgacgacac gatggacggc atcctcaact ggtaccgcga agagggcacg
     1201 atcttcaagg gcgggtctgg ttcgggaatc aacctgtcca agatccgctc cagccacgag
     1261 ctgctccagg gcggtggcac cgccagcggc ccggtcagct tcatgcgcgg cgccgatgcg
     1321 tcgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc aggccgtggt cgacgacgcc gattggaatc
     1561 tggtcgcggt caccgacggc cgggtgatcc gcaccgtcaa ggctcgcgag ctgtggcgcc
     1621 aggtcgccac cgcggcgtgg gagtgcgccg accccgggct gcagttcgac accaccatca
     1681 acacctggca caccgcgcac gccacgggcc gcatcaacgg ctccaacccg tgcagcgagt
     1741 acatgcacct cgacaactcg gcctgcaacc tggccagcgt caacttgctc aagtacctcg
     1801 acgacgacgg caccttcgac atcgaggcct acaaggccac gatcgannnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnna acctcggtgc gctgctgatg gcgctcgggt
     1981 tggcctacga ctcgcccgca ggccgggcct gggccgcggg gctcacgtcg ctgatgaccg
     2041 gccacgccta cgccaccagc gcccgcatcg ccagccggat ggggccgttc gcgggcttcg
     2101 ccgagaacga gagctacatg ctcaacgtgt tgcgcatgca ccgcgacgcc agctaccgca
     2161 tcgaaggtgc cgacatcgtg ccgaccgaac tgctcttcgc cggccagcag gcctgggagg
     2221 ccgcggtggt cgacggcgag gagtacggcg tgcgcaacag ccaggccacg gtgctcgcac
     2281 ccaccggcac gatcggtctg atgatggact gcgacaccac cggcatcgag cccgacctcg
     2341 gcctggtcaa gatgaagaag ctggtcggcg gcggcaacat gagcatcgnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnggcgt tgcgcacgtt gggctacacg cccagccagg
     2461 tggacgacat catcgcctac atcgcggagg agaagagcat cctcggcgcg ccgcacatcg
     2521 cgcccgaaca catcgcggtg ttcgcgtgca gcatgggcga caacaccatc cactacgagg
     2581 gccacgtgcg gatgatgggc gcgacccagc cgttcctgtc gggtgcgatc agcaagaccg
     2641 tcaacatgcc cgaggacgcc accatcgaag acgtcgaggc actgcacctg ctgtcgtggc
     2701 ggcttggcct caaggcagtc gcgatctacc gcgacaactg caaggtcgcc caaccgctgc
     2761 agaccgccaa gaagggcgac gccaccgtcg acgccgaagg acgtctcgtc gcggccacca
     2821 acggtggtgc ggccccggtc gtcgcgaccc agacggtcga gcggatcgtc gaacgcatcg
     2881 tccaccagcc cgtgcgccag aagctgccgc gcagccgccg cggccgcacc ttcgagttcc
     2941 gcgtcgccga ctgcaagggc ttcgccacca tcggcgagta cgacaacggt cagccgggtg
     3001 agatcttcct caccgtgtcc aagcagggtt ccacactcag cggcatcatg gacgcgttcg
     3061 cgaagagcat cagctacggc ctgcagtacg gggtgccgct gcgggcgttc gtcgaggcgt
     3121 tcaccaacat gcgcttcgag ccggcgggca tgaccgacga cccggacatt cgcttcgcat
     3181 cgtcgatcat ggactacctg ttccgccgcc tcgcgctgga gtacctcacc tacgacgagc
     3241 gggccgagtt gggcatcttc tccgtcgacg agcgcctcca gccgacgctg ccgggcgtct
     3301 acgagagcac catcgagaac cgcaccggca gcgaggttgc cgccgatccg aagtcgatcc
     3361 cgtcggccgg tgagttgacg gtctcgctcg agctcggcct cgctcccgag gcaccgcgca
     3421 gcgacaacac ccccgctgcg ggtatccctg ccgcgcgtca gagcgattcc ccgatgtgca
     3481 tgcagtgcgg cgtccagatg atccgcgccg gtagctgcca cgcctgcccg agctgcggca
     3541 gcaccagcgg ctgcagctaa cccgcaacgg ctcgatgtgt gttcgattcg cccccagggc
     3601 cggggtgaat cgaacaccgt gccgcgctgg ttgcgcatgt gtgttcacgg gacccctcca
     3661 ggacgggtaa atcgaacgca gtctcagcgg cgggcgtcag gtgtgttcat ttgacccgcc
     3721 cagtaagggt gaatcgaaca cgccgacggg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cgagatgcga ccaccgaagt
     3901 ctcgaccacg accgccatca cgaccacgac gggggcagtg ccggtgacgg gagagttgta
     3961 catcgatacg ttcgaggtgc tccccgatcc agcgacggtg ccgtggttgg agccggtggt
     4021 atcgccgcca cggctgcggt tgctggcggc agagcggggc tacgccacga cgttcgtgtg
     4081 cccgccgaat gcgtcgtgcg tgccgaacgt ggccaaggcg acgctgcgct atggggccga
     4141 cgaggtgggc tcggagcgac gcgtgctgat cgagcagtac ttcggcgagg cgaccgccga
     4201 cctggagccg cagccgaacg acacgatcgg cgtccgctcg gtggagcgtg gcgcgggtga
     4261 ggtgattcgt tggcgcgagg agagcggcgt gttggtggtg gtgtcaccgc tcggcggcat
     4321 cgcgagcgac gagatcaccg cgatcgtcga ctcgctccag gtcggtacga ccgacgtctg
     4381 gccccgttgg ggcatcgaag ctcctgcgat gctgtgcgtc gatgctgcgt cgcggtatgc
     4441 gccgttgacg ccggaggggt ggcagcggtt cgtgctcgac gtggtacctg acggcgggtg
     4501 caccggccac accgtgctgt tcatgtcgtt ggtgttgccg ccgaccgacg acgatgccgg
     4561 ggcgttggcg acgttcgtct tctctgctga tccgacgtca tcgcgcaccc ggccagatga
     4621 cgcagagacg gtcgagatcg acggcatcac ggtgacgatc gatcgcagcg tccgcgacgt
     4681 ccgtggcctt cccgcggtcg gcgtcgtgat gccgttcgga ccggtgagcc tcgacgccca
     4741 cggctacctg tcgccagagc gactcgcggc gctggtgcgc accgtcgccc tggtcgacgc
     4801 aaccgagtgg gccaccctgc aggccgaagc cgcggccatc cgcgaagcgg cgggtaccac
     4861 gaccgacacc atcgacatcc cccaagccac cgatgacgtc acggtgcctg gtggcggttg
     4921 acggcatcca cgttgcgccg gcacggggtt atcggtccac gctcgcgacg ctgttcggcc
     4981 cggcgaccta ccctgcgccg agcacggtga ggccagcgga gcgcgagtga gtcgcgagct
     5041 tggcatcgaa ggtcgcgatg gcacggcgct gacgagcgtt tcgatgggtg cggcccctgg
     5101 accggttgcg gatcaatagc ctggagccat gactgcaacc gatgaccgca ccgcacctgc
     5161 cgacgacgct gcccgtgatc tggccgcgct cggggtgcac tgggatctcg aaccgctgct
     5221 cgacggcgcg gcggacatcg acgagttgct cgaccaggcc gatgcgatcg ccgacgagcn
     5281 nnnnnnnnnt ccggccgggg tcgcatcgcc accatggcgg cagccgagct cgtcacgttc
     5341 catcgtcgtt acgccgagct gatcgaattg atgggacggg cgggcaacta cgccagcctg
     5401 cgcttcgcgg ccgacacgtc ggtgcccgcc aacggcgcgc tgatggcgcg ggcccaggaa
     5461 cgctcgaccg cgatcagcac caagctgttg ttcgtcgagc tcgaatgggc cgcggtcagc
     5521 gacgagcagg ccgaggcggt gctcgccgat ccctcgcccg agctcgactt cattcgccac
     5581 caccttcgct ccagccgccg ctacatcccc tacctcttga gtgagcccga agagcgggtg
     5641 ctgaccgaga agagcgtcac cggtgccggc gcgtggacgc gtctgttcga cgagctgacc
     5701 gcggcgatcg aggtcgacgt ggtgcagccc gacggtaccg ctcggacgat gccgctgatg
     5761 caagggctgg cgctgatgca gcaccccgac cgggcggtac gtcagcacgc gcacgccgcg
     5821 gtcaccgagg ggctggcccc cgggttgcgg actcgggcct tcgtgttcaa caccctgctc
     5881 aacgacaagt cgatcgacga ccgcctgcgg aactatcccg actggatcac cagccgcaac
     5941 ctggccaacg aggccagtga ccagagcgtg caggcactga tcgacgcggt ggtgtcgcgc
     6001 tacgacatcc cccagcggtg gtacgcgctc aaggccacgt tgctcggtgt cgagcggctc
     6061 gccgactacg accgctacgc cagcgtggcc ggcaccgagc gggagatccc gtggtccgtc
     6121 gcgaccgaga tcgtcaccga cgcgtactcg tccttcagcg gagagttggc gtcgatcgtg
     6181 cagcgcttcc tgcgtgaagg gtggatcgac gcgccggtca cacccggcaa gcgcggcggg
     6241 gcgttctgcg cgtacaccgt gccgtcgcac cacccgtacg tgatgctcaa ctggaccagc
     6301 cgcacgcgtg acgtcgcgac gttggctcac gagctgggcc acggcgtgca cggttacctg
     6361 tcgcgcgagc agggcgtgtt ccagatgtcg acgccgctca cgctggccga gacggcatcg
     6421 gtgttcggcg agacggtcac cagcaagcgg ctgctggcca gcctgaccga tccgggcgag
     6481 cggctggcgc tgctggctgc gacgctcgag gactcgatcg ccaccgtgtt ccgtcagatc
     6541 tcgatgaacc gcttcgagca agccgtgcac acggctcggc gggcgcaagg tgagctgtcg
     6601 gtcgacgcgt tcaacgagct gtgggtcgaa tcgcaggccg cgatgttcgg cgattcgatc
     6661 gagatcaccg agggctaccg cacgtggtgg agctacatcc cgcacttcat gcacaccccg
     6721 ggctacgtgt acgcctatgc ctacgggcag ttgctggccc tcagcgtgta caagcgctac
     6781 gaggaggtgg gtgacgagtt cgtgccgcgg tatctcgacc tgttgcgcgc cggtggttcg
     6841 atgactccgg aagatctcgg ccgcatcgtc gactgcgacc tcggcgaccc ccagttctgg
     6901 accggcggcc tggccatcgt cgacgaacag ctcgtcgctg ccgaacaagc cgccgctgcc
     6961 gtcgcccgct cctgacacca cgatctttcg ttacctggca tcgttcgcga tctcgttctc
     7021 gctctcgctc actgcgctgt caggaacgcg gtgaccgagc gtggggcgtg agcggagccc
     7081 ccactggccg gtgcgcgccg ttgcgatcag cggcgtctga gggcatcgct gcatgcacgg
     7141 ccggtcggag cgggtctgac agactggccg cgtgcgccgg ggactgccac tgctgatcgt
     7201 tgtgggactg gccgcgtcgc tggccgcctg cggacgcgac ggcggtgcgt acgcggtcag
     7261 cgacacgctg cccgacgtgg tgaccctcgg cggtctgccc gagttcggcg acgcgcccga
     7321 gaccgcgatg gcagagaccg agccgag
//
LOCUS       DAORNF010000056         7345 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_7485_length_7345_cov_1.862689, whole genome shotgun sequence.
ACCESSION   DAORNF010000056 DAORNF010000000
VERSION     DAORNF010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 7345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7345
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7485_length_7345_cov_1.862689"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1177)
                     /locus_tag="PLV68_03490"
     CDS             complement(<1..1177)
                     /locus_tag="PLV68_03490"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HRE00334.1"
                     /translation="MTGSDPPPDQHQDGTDAEAAPEPALGAALNAALTEDADAQPVGP
                     VAAVLRMPSWVWKSVAIFWLGFLATIFGRFLFARLSSLLLLLLVSVFLSLAIEPGVNR
                     LAARGWRRGRATMLILMGSLGVALLFVIVIGAQVGSQIADLLANSSQTVTKTVDGIND
                     LLGTHIDASDVIDAIQDDDGPVQQFIQSQQNKVFDLSLTALGFLLQLFSVLLFTFYLV
                     ADGPRMRRSICSRMRPTRQEQVLHTWELAINKTGGYLYSRALLAGLSAVFHWILFQAI
                     GLDAPIAMALWVGLISQFLPVVGTYIAGALPILLALIDSPFTAIAVLAFVALYQQIEN
                     YLFAPRITARTMELHPAIAFGAALGGAALLGGVGAILALPAAAMAQALISDWGSRHEV
                     "
     gene            complement(1174..>2271)
                     /locus_tag="PLV68_03495"
     CDS             complement(1174..>2271)
                     /locus_tag="PLV68_03495"
                     /inference="COORDINATES: protein motif:HMM:NF017984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HRE00335.1"
                     /translation="RIGPIQIDSVNVLARSQELVLFARLGPHPRDLLPRAAAAGELFE
                     YWSHEATHVPSAHHHLHRWKMASPHRWKSIDAVRERRPDVFADVLQRIRANGPMVAGE
                     FNARVGPKGAWWDWDDAKIALEHHFWTGALTARRRANDFARVYELTERWLPAAALAHP
                     TPTEHEARKELLMLAAAHHGVATATDLADYHRQRMPVVRPLVDELVAEERLIPVTVEG
                     WTHPAYLHPLAKRPRKVQARALLSPFDSLIWDRERTERLFDFHYRIEIYVPPPKRRYG
                     YYVLPFLLGQDLVGRVDLKADRQAGVLAVQGAFAEPGIQPGPIAADVAGELADELIEM
                     AGWLGLGGVQVAQRGDLANPLRDAVRVRSGS"
     assembly_gap    2272..2307
                     /estimated_length=36
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2391..>2632)
                     /locus_tag="PLV68_03500"
     CDS             complement(2391..>2632)
                     /locus_tag="PLV68_03500"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRE00336.1"
                     /translation="QRYQIGSLAMANAGPNTNGSQFFLISGPSGVGLPPQYNHFGQIV
                     KGLDVLDTMQKVATDRGDRPREDVVINSVAIAVAD"
     assembly_gap    2633..2642
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2643..2876)
                     /locus_tag="PLV68_03505"
     CDS             complement(<2643..2876)
                     /locus_tag="PLV68_03505"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRE00337.1"
                     /translation="MGIDPSKRYSATLDTSLGQIVIALDAVNAPKTVNNFVFLALHHY
                     YDGVIFHRIINGFMCQGGDPTGTGRGGPGYQFAD"
     gene            complement(2956..>3328)
                     /locus_tag="PLV68_03510"
     CDS             complement(2956..>3328)
                     /locus_tag="PLV68_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HRE00338.1"
                     /translation="GLTVGLTLTLIHMISIPVDNTSVNPARSFGAALFADGDTDALNQ
                     LWAFVVFPLIGAVVGVFVWLMLDDTRLEDTLLDNEALREIRDVADQAMDEVVEATTGL
                     PAPDEGPPCTDRAAAVIGPGR"
     assembly_gap    3329..3445
                     /estimated_length=117
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3446..3799)
                     /locus_tag="PLV68_03515"
     CDS             complement(<3446..3799)
                     /locus_tag="PLV68_03515"
                     /inference="COORDINATES: protein motif:HMM:NF012455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HRE00339.1"
                     /translation="MGAEAFGTTVLMLGGPGTAVLMPDFAGKQLAVALAFGFSLLIMA
                     YVIGPISGCHINPAVTLGMWLTKKVNAAHALFAFVGQVIGAALGGFLIWTIARGVDGW
                     EPGRFASNGYGTAHSP"
     gene            3920..>4185
                     /locus_tag="PLV68_03520"
     CDS             3920..>4185
                     /locus_tag="PLV68_03520"
                     /inference="COORDINATES: protein motif:HMM:NF014671.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HRE00340.1"
                     /translation="MARRRTEKLLVTRVVAGGERRRRPDDVIVEEPLSIQLDGQLVTT
                     TMRTPGHDYELAVGFCHAEGLLGSAPVIGVRYCANGSAAASEFNV"
     assembly_gap    4186..4255
                     /estimated_length=70
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4256..4749
                     /locus_tag="PLV68_03525"
     CDS             <4256..4749
                     /locus_tag="PLV68_03525"
                     /inference="COORDINATES: protein motif:HMM:NF014671.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HRE00341.1"
                     /translation="GSDSIDTLAARLAPVAAGAWHGFDVDTFPALCAAALDQQGLFAL
                     TGAVHGAAAFDREGNVLIAREDVGRHNAVDKVVGRLLLDGRLPADGLGLFVSGRASFE
                     MVQKAWAGGFAVVLSVSAPTALAVQTARRANLLLAGFVRGDHLNIYAPDKLPTPAVKS
                     VTT"
     gene            4746..5453
                     /locus_tag="PLV68_03530"
     CDS             4746..5453
                     /locus_tag="PLV68_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HRE00342.1"
                     /translation="MTDSSNASNTPDLGDDAVVPTTGLTVLHLFCHVDGDVDDAAMQA
                     CVAAAQAEGAQVVAAQMLGHKADVAFMALHPDMRVLHRLQRGLRRAGLGIGASYVSIT
                     EVSEYTKGMPAEMLSPRLYPVLPPAGKPAFCFYPMTKRRNADANWYTLPYDERLALMH
                     EHGGSGRKFAGRITQLITGSTGLDDFEWGVTLFGVHTDDIKDVVYTLRFDRASALYGE
                     FGDFYVGAVTPIDQLLA"
     gene            5860..>7345
                     /locus_tag="PLV68_03535"
     CDS             5860..>7345
                     /locus_tag="PLV68_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00343.1"
                     /translation="MDPSIESASDLEPVPTEQLWPDEVWTDASGAGDGRHITDDAEGD
                     TPDLVPRRGAMLRLAAIGLVLIVVLATITGAWSPDRWTATEVEMVAGDPGPADLLVPE
                     VTQAVSQTTPRSERDASETSASSTSEPVPASTDLVDAGETPVPGSVAPPIGGGGEQAP
                     ASTTVTDPALVARPIPEEVTATTTAPSPWAAVSRTTSAGHVATDVGCASGTSAGALDA
                     FFAQRIGPVLGHDYQHVYALGGHRYLWLFQDTFVDYSAAATTLDKAAFTHNTAMIQEG
                     ACFTLLHRGTIARPTSFEQGTGEARLGKWFWPMGGQTFGNRVYVFWAEMQKDGYDPKP
                     PNGLGWHPARTWLAVYDVATMARVSFQAAPNSGVLPIYGYAVDSDAEYTYLFGNTFEQ
                     NLQREGGYYNGPHSATKMFVARVPLGRFDQPLQYRSGDDWTYDATAATPIVQRYWAEN
                     PMQPRYLGGQWVAVTKVDGYWGESLSVDVANDPWGPWTTTESRRI"
BASE COUNT         1137 a         2358 c         2484 g         1133 t
ORIGIN      
        1 ccacctcgtg gcgcgagccc cagtcgctga tcagcgcctg ggccatcgcc gccgcgggca
       61 gggccaggat cgcgccgacg ccgccgagca gcgccgcccc accgagcgcc gctccgaacg
      121 cgatcgcggg atggagttcc atcgtgcggg cggtgatccg cggtgcgaag aggtagttct
      181 cgatctgctg atagagcgcg acgaaggcca gcacagcgat cgccgtgaag ggcgagtcga
      241 tcagggcgag caagatcggc aacgcacccg cgatgtacgt gccgaccacc ggcaggaact
      301 ggctgatcag accgacccac agcgccatcg cgatcggggc gtcgagcccg atcgcttgga
      361 acaagatcca gtggaacacc gccgataggc ccgccaacaa cgcccgcgag tacaggtagc
      421 cgcctgtctt gttgatggcc agctcccagg tgtgcaacac ctgctcttgg cgcgtcgggc
      481 gcatccggct gcagatgctg cgccgcatcc gcggcccgtc ggccaccaga taaaaggtga
      541 acagcaacac cgagaagagc tgcaacaaga agcccagcgc ggtcagcgac aggtcgaaca
      601 ccttgttctg ctgcgactgg atgaactgtt gtaccgggcc gtcgtcgtct tggatcgcgt
      661 cgatcacgtc ggacgcgtcg atgtgcgtgc cgagcaggtc gttgatgccg tcgacggtct
      721 tggtgaccgt ttgcgacgag ttggccaaca ggtcggcgat ctgcgagccg acctgggctc
      781 cgatcacgat cacgaacaac agcgcgaccc ccagcgaacc catcaggatc agcatcgtcg
      841 cccggccccg gcgccacccg cgggccgcga ggcgattgac gcccggctcg atcgccaacg
      901 acaggaacac cgacaccaac agcagcagca gcaggctgct gaggcgagcg aacaggaagc
      961 gtccgaagat cgtggccaag aagcccagcc agaagatggc gaccgacttc cacacccacg
     1021 atggcatccg caacacggcg gcgaccggac ccaccggctg cgcgtcggcg tcctcggtca
     1081 acgccgcgtt caacgccgca cccaacgcgg gttccggcgc agcctccgcg tccgtcccgt
     1141 cttggtgctg gtcgggaggc ggatcgctgc cggtcacgag ccggatcgta ctcgtacggc
     1201 gtcgcgcagt gggttggcca gatcgccgcg ttgggcgacc tgcactccgc cgagtccgag
     1261 ccaaccggcc atctcgatca gctcgtcggc caactcgccg gcgacgtcgg cggcgatggg
     1321 gccgggctga atgcctggct ccgcgaacgc gccctgcacc gcgagcacac cggcctggcg
     1381 gtcggccttg agatcgaccc ggccgaccag gtcttggccg agcaggaacg gcagcacgta
     1441 gtagccgtag cggcgcttcg gcggcgggac gtagatctcg atgcggtagt ggaagtcgaa
     1501 gagccgctcg gtccgctcgc gatcccagat cagcgagtcg aatggactga ggagggcccg
     1561 cgcctgcacc ttccgcggcc gcttggccag cgggtggagg tatgcgggat gggtccagcc
     1621 ctcgaccgtg accgggatca gccgttcttc ggcgacgagc tcatcgacca gcgggcgcac
     1681 gaccggcatc cgttggcggt gatagtcggc caagtcggtc gcggtggcca cgccgtggtg
     1741 cgccgcagcc agcatcagca gctccttgcg ggcttcgtgc tcggtgggcg tggggtgggc
     1801 gagggcagcc gcgggcagcc aacgctcggt cagttcgtag acgcgggcga agtcgttggc
     1861 acgccggcgg gcggtgaggg cacctgtcca gaagtggtgc tcgagcgcga tcttggcgtc
     1921 gtcccagtcc caccaggcgc ccttgggtcc gacccgggcg ttgaactcgc cggccaccat
     1981 cgggccgttg gcgcggatgc gttgcagcac gtcggcgaac acgtcggggc gtcgttcgcg
     2041 cacggcgtcg atcgacttcc agcggtgcgg cgaggccatc ttccagcggt gcaagtgatg
     2101 gtgcgcgctg ggcacatgcg ttgcttcgtg cgaccagtat tcgaacagct cgccagcagc
     2161 agccgcgcgc ggcagcaggt cgcgggggtg cgggcccagt cgggcgaaca gcacgagctc
     2221 ttggctgcgg gccaacacgt tgaccgaatc gatctggatc ggaccgatac gnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnngac gagcgcatgg tctggttgct ggtcgcggcg
     2341 gtggtgatcg gcgcgcccat tgtcgaagaa ctggtattcc gcgtcggtgt tcagtcggcg
     2401 acggcgatgg cgaccgagtt gatcaccacg tcttcacggg gacggtcgcc acggtcggta
     2461 gccaccttct gcatcgtgtc gagcacgtcg agacccttca cgatctgacc gaagtggttg
     2521 tactgcggcg gcaggccgac cccgctgggg ccgctgatga ggaagaactg gctgccgttg
     2581 gtgttgggac cagcgttggc catggcgagc gacccgatct ggtagcgctg ctnnnnnnnn
     2641 nngtcggcga actgatagcc gggaccaccg cggccggtac cggtggggtc gccgccctgg
     2701 cacatgaacc cgttgatgat gcggtggaag atcacgccgt cgtaatagtg gtggagcgcg
     2761 aggaagacga agttgttgac cgtcttgggc gcgttgacgg cgtcgagtgc gatcacgatc
     2821 tggcccaggc tggtgtcgag cgtggccgag tatcgcttgg acgggtcgat gcccatctcc
     2881 ggggcttcgt tgaacttctg cttcttgtcc gcggaaccgt cgaacggggg gaaggaatgt
     2941 gccatgcgca gcaggctaac ggcccggccc gatgacggct gcggcccgat cagtgcacgg
     3001 cgggccttcg tcgggggcgg gcagcccggt ggtcgcttcg acgacctcgt ccatcgcttg
     3061 gtcggcgacg tcgcggatct cgcgcagcgc ctcgttgtcg agcagcgtgt cttccaggcg
     3121 ggtgtcgtcg agcatcaacc acacgaacac gccgaccacc gcgccgatca gcgggaacac
     3181 cacgaaggcc cacagttggt tgagcgcgtc ggtgtcgccg tcggcgaaca aggcggcccc
     3241 gaagctgcgt gccgggttga ccgaggtatt gtcgaccggg atcgagatca tgtggatcag
     3301 ggtcagcgtg agcccgaccg tcaaaccgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnaggtg agtgcgcggt gccgtacccg ttggaggcga
     3481 accgtcccgg ttcccatccg tcgacaccgc gggcgatcgt ccagatcagg aagccgccga
     3541 gcgcggcccc gatcacctga ccgacgaacg cgaacaaggc gtgtgcggcg ttgaccttct
     3601 tggtcagcca catgcccaag gtgaccgccg ggttgatgtg gcagccgctg atcgggccga
     3661 tgacgtaggc catgatcagc agcgagaacc cgaacgcgag ggcgacggcc agctgcttgc
     3721 cggcgaaatc gggcatcagc accgcggtgc cgggaccacc cagcatgagc accgtggtgc
     3781 cgaatgcttc ggctcccaag attcgtgcgt tgttctgcat tgcctctcct cgtccgacgc
     3841 gatgcacgga cacacgtgcg gcgcgcctct cacggcgtta cgggctcacg ttaggcaacc
     3901 tcggtagcgt ggggcggtga tggcgcggcg tcgcaccgag aaattgttgg tcacccgggt
     3961 ggtggccggt ggcgagcgcc gccgccgccc cgacgacgtg atcgtcgaag agccgctgtc
     4021 gatccaactc gacggccagt tggtgaccac caccatgcgc acccccggcc acgattacga
     4081 gctcgccgtg ggattctgtc atgcggaagg actgcttggc tccgccccgg tcatcggcgt
     4141 gcgctactgc gccaacgggt ccgccgcggc cagcgagttc aacgtnnnnn nnnnnnnnnn
     4201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngtggc
     4261 agcgacagca tcgacacgtt ggccgcccgg ctcgcgccgg tcgctgcagg cgcctggcac
     4321 gggttcgacg tcgacacctt tcccgctctg tgcgcggccg cgctcgacca gcagggtctg
     4381 ttcgcgctga ccggtgcggt gcacggcgca gcggccttcg accgcgaggg caacgtcctg
     4441 atcgcgcgcg aagacgtcgg acgtcacaac gcggtcgaca aggtcgtcgg acgcctgctg
     4501 ctcgacggcc gcttgcccgc ggacgggctg ggcctgttcg tcagcggtcg ggccagcttc
     4561 gagatggtgc aaaaggcgtg ggctggcggt ttcgccgtcg tgctcagcgt cagcgctccc
     4621 acggcgttgg ccgtgcagac tgcccggcgt gccaacctgt tgttggccgg cttcgtgcgc
     4681 ggcgaccacc tcaacatcta tgcccccgac aagttgccca cccctgcagt gaagagcgtg
     4741 acgacatgac cgattcgagc aatgccagca acacccccga tctcggcgac gacgctgtcg
     4801 tgcccaccac cggcctgacg gtgctgcacc tgttctgtca cgtcgacggc gacgtggacg
     4861 acgccgcgat gcaggcctgc gtggccgcag cgcaggccga gggggcgcag gtggtggcgg
     4921 cgcagatgct gggccacaag gccgatgtcg cgttcatggc gttgcacccc gacatgcgcg
     4981 tgctgcaccg tttgcagcgc gggctgcgcc gggccgggct cggtatcggg gccagctacg
     5041 tgtcgatcac cgaggtcagc gagtacacca agggcatgcc cgcggagatg ttgtcgccgc
     5101 ggctgtatcc ggtgctgccg ccggccggca agcccgcgtt ctgcttctat ccgatgacca
     5161 agcgccgcaa cgccgacgcc aactggtaca cgctgccgta cgacgagcgg ctcgcgctga
     5221 tgcacgagca cggcggcagc gggcgcaagt tcgctggacg gatcacccag ctgatcaccg
     5281 gttcgaccgg cctcgacgac ttcgagtggg gcgtcacgct gttcggcgtg cacaccgacg
     5341 acatcaaaga cgtcgtctac accttgcgct tcgaccgcgc gtcggcgctg tacggcgagt
     5401 tcggcgactt ctacgtcggc gcggtgacgc cgatcgacca gctgctcgcc tgatccggct
     5461 cgcctgatcc ggcttcagcg gtcgctgctc agctggcgag cgcggccagc tctgggctgg
     5521 tcgcggcaca gcatgcgggg cagagctgat cgtcgtcgca gcacaacagg ggcaggtcgc
     5581 tgcaggcgag ctcgagcgac tcgcgcaccg atcgccggtg cttgggcctc cgtcccgtga
     5641 tgtgctggct caacccgagc cagccgcggt caggcttgct ggtgtggtgg ttcaactccg
     5701 gactctcctg atcgccggtc tccgttgcgc tgaccgtgcg gtcaggtggc gggcactgtg
     5761 catgttatgc acccttcccg agttcgcgtc tcgccagacg cgtgccggtc cggcgagggc
     5821 gatggggacg atgtcgccaa cagtggtatt tttcttcgag tggacccgtc gatcgagtcg
     5881 gcgtccgacc tcgagccggt gccgacggaa cagctgtggc ctgacgaggt atggacagac
     5941 gcgtctggtg ccggcgacgg gcgccacatc accgacgacg ccgagggtga cacgcccgat
     6001 ctggtgccgc ggcggggggc aatgctgcgg ttggccgcga tcggactggt cctgatcgtc
     6061 gtgttggcca cgatcaccgg tgcctggtcg cccgaccgct ggacggcgac cgaggtcgag
     6121 atggtggccg gtgaccccgg cccggccgac ctgctcgtcc cggaggtgac gcaggccgtg
     6181 tcccagacca cccctcgatc ggagcgcgac gcgtcggaga cgtcggcgtc gtcgaccagc
     6241 gagccggtgc cggcatcgac cgatctggtc gatgccggtg agacacccgt gccgggcagc
     6301 gtcgccccac cgatcggcgg cggcggcgaa caggcgcctg cgagcaccac ggtcaccgac
     6361 ccggcgctgg tggccaggcc gattcccgaa gaggtcaccg ccaccaccac cgccccgtca
     6421 ccgtgggccg ccgtgtcacg cacgacatcg gccggccacg tcgccactga tgtcggttgc
     6481 gcgtcgggta cgtccgcggg cgcgctcgac gcgttcttcg cccagcggat cggtccggtg
     6541 ctcggtcacg attaccagca cgtctacgcc ctgggcggtc accgctacct gtggctgttc
     6601 caggacacgt tcgtcgacta cagcgccgcc gcgaccaccc tcgacaaggc cgcgttcacc
     6661 cacaacacgg caatgatcca agagggcgcg tgcttcacct tgttgcaccg aggcacgatc
     6721 gcccgtccga cctcgttcga acagggcacg ggcgaggccc ggttgggcaa gtggttctgg
     6781 ccgatgggtg gtcagacgtt cggcaaccgg gtgtacgtgt tctgggccga gatgcaaaag
     6841 gacgggtacg acccgaagcc gcccaacggg ctcggctggc atccggctcg tacttggctg
     6901 gcggtgtacg acgtcgcgac gatggcccgc gtctcgttcc aggccgcgcc caacagcggg
     6961 gtgttgccga tctacggcta cgcggtcgac agcgacgccg agtacacgta cctgttcggc
     7021 aacaccttcg agcagaacct gcagcgcgag ggcggctact acaacggtcc gcactcggcg
     7081 accaagatgt tcgtggcgcg ggtgccgctc ggacggttcg accagccgtt gcagtaccgc
     7141 tcgggcgacg actggaccta cgacgccacc gcggcgacgc cgatcgtgca gcggtactgg
     7201 gccgagaacc cgatgcagcc gcggtatctg ggcggccagt gggtggcggt caccaaggtc
     7261 gacgggtact ggggcgagtc gctgtcggtc gatgtcgcca acgacccgtg gggtccgtgg
     7321 accaccaccg agtcgcggcg catct
//
LOCUS       DAORNF010000057         7323 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_7511_length_7323_cov_1.811227, whole genome shotgun sequence.
ACCESSION   DAORNF010000057 DAORNF010000000
VERSION     DAORNF010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 7323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7323
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7511_length_7323_cov_1.811227"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(101..1672)
                     /locus_tag="PLV68_03540"
     CDS             complement(101..1672)
                     /locus_tag="PLV68_03540"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRE00344.1"
                     /translation="MGRKILFVTTDQQRYDTLGCNGGTVARTPVLDGLAANGVRFERA
                     TPQSVVCMPSRSTIITGQYPSTHGVWMNGVPLPVDAPSVAAELNRSGYKTALVGKAHF
                     EPVLDPFAKFTENRLGREGKPTEYDEPWYDGTLGVHRGFDHLELATHSPNGQLHYSRW
                     IQQHHPEVIGGFYPVLDNLFEVNAAGGGDTGAPQAHHNPIAKEWYHTDWVADRTIAWL
                     DSLGASDDWFCWMSFPDPHHPWDPPSSEVGRIDWREVPLPEGYPQSAAERERILDGKP
                     RHWRLWYDGKLVSNYEAPKRFVPATLTADQIREINALNAVECELIDEALGRVLAAIAA
                     KGWADDLDVIFTTDHGEFQGDFGLMFKGPYHVDALMRLPLIWRPAPSAGVAPAVVTRP
                     VGLIDLAPTFATIAGLAPNEWMQGAPLPVDDRDADARRDDKVLTEWDSELWGVATRLR
                     TITRGQWVCTAYKPGYVHDGTEGELYDLTADPLQQTNLWDDPGYAAVRSDLVADLWDS
                     QPPVRTPRLALLAPV"
     gene            complement(1672..2169)
                     /locus_tag="PLV68_03545"
     CDS             complement(1672..2169)
                     /locus_tag="PLV68_03545"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HRE00345.1"
                     /translation="MATLRRDIVIGRSAADVWDVVGRPELLHHWFPGIVDCTVTYGDD
                     GTATRIITTGSGIPMPETIVTNDSLQRRFQYRITAPLFASHLATVDVIEVDESSCVVA
                     YSTEAEPSVMALVIGGGTSGALAELRRQMEAGDGPAITAARQTTPFIPFATTGSPMTN
                     PETEL"
     gene            complement(2169..3134)
                     /locus_tag="PLV68_03550"
     CDS             complement(2169..3134)
                     /locus_tag="PLV68_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloalkane dehalogenase"
                     /protein_id="HRE00346.1"
                     /translation="MRGQTMQVLRTPDERFAALADFPYAPHYVDVDAGDGSGAVLRVH
                     YVDEGPSDAAPVLLMHGEPSWAFLYRHMIPTLVAAGHRVIAPDLVGFGRSDKPAEQSD
                     YTYARQVGWMRDALFAHLDLTGITLFCQDWGGLIGLRLVAAAPDRFARVVVGNTGLPT
                     GESRLSEAFLSWQRFAATAPKFPVGNIINGGTVTDLTPEVIAAYDAPFPDDSYKAGAR
                     VLPSLVPSAPDDPASADNLAAWTVLESFTKPFLCAFSDSDPITKGGEGVFQRRVPGTA
                     GQAHTTIAGGGHFLQEDQGPLLAQVIADFIAATPTPGAAPDGGSR"
     gene            complement(3271..4488)
                     /locus_tag="PLV68_03555"
     CDS             complement(3271..4488)
                     /locus_tag="PLV68_03555"
                     /inference="COORDINATES: protein motif:HMM:TIGR02032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045550 - geranylgeranyl reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranyl reductase family protein"
                     /protein_id="HRE00347.1"
                     /translation="MNQRDLLVIGGGPAGSAAAIRAARGGARVTLFEKGPYGRDKVCG
                     DGLTPRAVAALNELAIDLSPAHRIDGLRMIAGKRRRELAWPVTDRFPNHGAVWPRHRF
                     DTHLIDVAIAAGVEVRFDTEALPELDGDRVVGVSADGEQFGAPLTVLAAGAQGAAAKR
                     VGAERVADEPFGLAIRAYAPSPRHADRHLEACLSLRDEHGTPVPGYGWMFPAGDGTVN
                     IGVGALSTMKGFKQLNLNTLLDQYAALVRDEWQLGAYLDKPRAWRLPMSCVRRHGDGW
                     LAIGDAAGFVNPMNGEGIDYGLESGTLAADLFLADPATAPQHYDREVGERFDRFLQTG
                     RRFSFIIGHPWLLRPGLRIAVGTQRAADITLSVMGNLIDGQTPGAAGRVLRMAGGVLR
                     AADPLLRRTRARA"
     assembly_gap    4639..4800
                     /estimated_length=162
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4801..5410
                     /locus_tag="PLV68_03560"
     CDS             <4801..5410
                     /locus_tag="PLV68_03560"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRE00348.1"
                     /translation="PPHPRIAFTEAGFSEVNHLYVENDPRLALSQPASLLVIGPRGPG
                     LAKALHLGSTAEWLITRPPSPMLIPRHGRVTRSVTVCHDGSSHATVATQALARMPWIE
                     GVAVTVLAVDDSRSDPSTAVEEATAILSDSGAVLDARMLVGDPTQVILRHLERHGTDL
                     LVFGTSGLTGLQRLRVGSTASALAHATDLSILVACDHSVYEA"
     gene            complement(5441..7216)
                     /locus_tag="PLV68_03565"
     CDS             complement(5441..7216)
                     /locus_tag="PLV68_03565"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HRE00349.1"
                     /translation="MKTINLKTASLVLTGVGLLSLARAVMFDTAPEGTHNIGLMQVQT
                     LWAQFASTMLVVGAIFVLTRDDNNGDAGPSTDRTDETDETDSTTSEPTAESLPPDQAA
                     YVADLDQLCRAVAGEIGSIDPDDADYFGRVINALEAGAGMLYELQPPDLLQTQHQAVT
                     TNVETTIGATDDLAAARAADDQDEIDSAERQLSDLSNELDQLAAEIGAHECENLGVIG
                     GGDGSTPATDPDTTDPDTTDPSTPNTADSLPDTESYQVLHELAGHNARVVTAHFNGDG
                     TLLITSSTDGTARIWATDTGETFHVLDGHGGEVYDALFGPDNGHAATVGSDGVVRWWE
                     VANGALLAELTGHTDVVYEAEYSVPDGGSLLTASADGTARLWDLNTRSEAVRYTGATE
                     RLYFAQFSNDLQRVVGGGQDNTVLVWPLTDGSNPLRLTGHTGDVQLADFASDGERFLT
                     AADDGTVLVWNFDGVQLGSFGTIAGGVDDAEFSPDGTLIVACGKDSTLYVWDLTTETP
                     FQIAGHTGEIFSCAFSADGGRIVTASADGTAAVWDVATLLGGEGDPRLAVLAGTSPVM
                     AAEFSPDGSMIVTAEASGAVTLWAG"
BASE COUNT         1099 a         2445 c         2470 g         1147 t
ORIGIN      
        1 gccggttgca gcgggggcag atgaacgggc tctcgccagc gctgaagtcg agcgaaccga
       61 gcggcgcgtc catgagacgc caccgtagcg gccgaagggg tcagaccggg gcgagcagcg
      121 ccaggcgcgg ggtgcgcacc ggtggctggc tgtcccacag gtcggcgacc aggtcagagc
      181 gcaccgctgc gtagccgggg tcgtcccaca ggttggtctg ctggagcggg tcggcggtca
      241 ggtcgtacag ctcgccctcg gtcccgtcgt gcacgtaccc cggcttgtac gcggtacaca
      301 cccactgacc gcgggtgatc gtgcgcaggc gcgtcgctac gccccacagc tcgctgtccc
      361 actcggtcaa caccttgtcg tcgcggcgcg cgtcggcgtc gcggtcgtcg accggcagcg
      421 gcgcaccctg catccactcg ttcggggcga gaccggcgat ggtggcaaag gtcggtgcca
      481 ggtcgatcag cccgaccggg cgggtgacga ccgcgggtgc aacaccggcc gacggcgcgg
      541 gacgccagat cagcggcagg cgcatcagcg catcgacgtg gtacggaccc ttgaacatca
      601 acccgaagtc accctggaac tcgccgtggt cggtggtgaa gatcacgtcg agatcgtcgg
      661 cccagccctt ggcggcgatc gccgcgagca cccggcccag tgcctcgtcg atcagttcgc
      721 actcgaccgc gttgagcgcg ttgatctcgc ggatctggtc ggcggtcagc gtcgctggaa
      781 caaaccgctt cggcgcctcg tagttggaca ccaacttgcc gtcgtaccag agccgccagt
      841 ggcgcggctt gccgtcgagg atccgctcgc gctcggcggc cgactgcggg tagccctccg
      901 gcagcggcac ctcgcgccag tcgatgcggc cgacctcgga cgacggcgga tcccacgggt
      961 ggtgtgggtc ggggaagctc atccagcaga accagtcgtc gctggcgccg agcgagtcga
     1021 gccaggcgat cgtgcggtcg gccacccaat cggtgtggta ccactccttg gcgatcgggt
     1081 tatggtgcgc ttgcggagca ccggtgtcgc caccgcccgc cgcgttgacc tcgaacaggt
     1141 tgtcgagcac cgggtagaat ccaccgatca cttcggggtg gtgctgctgg atccagcggc
     1201 tgtagtgcag ttgaccgttg gggctgtggg tcgccagttc caggtggtcg aacccacggt
     1261 gcacaccgag ggtgccgtcg taccacggct cgtcgtactc ggtgggcttg ccctcccggc
     1321 cgaggcggtt ctcggtgaac ttggcgaacg gatcgagcac cggctcgaag tgggccttgc
     1381 cgaccagcgc cgtcttgtac ccggagcggt tgagctcggc cgccaccgac ggcgcatcga
     1441 ccggcagcgg cacgccgttc atccacacac cgtgcgtgct ggggtactga ccggtgatga
     1501 tggtcgaacg ggacggcatg cagaccaccg actgcggcgt cgctcgctcg aagcgcacac
     1561 cgttcgcggc caggccgtcg agcaccgggg tgcgggccac ggtgccgcca ttgcagccca
     1621 gcgtgtcgta gcgctgctgg tcggtggtga cgaacaagat cttgcgaccc atcacagctc
     1681 cgtttcgggg tttgtcattg gagatccagt tgttgcaaac ggaatgaagg gcgtggtctg
     1741 gcgagcggcg gtgatcgccg gtccatctcc ggcctccatc tggcgccgca gctcggcaag
     1801 ggcgccgctg gtgccgccgc cgatgaccaa ggccatcacc gagggttccg cttcggtcga
     1861 gtaggcgacg acgcacgacg actcgtcgac ctcgatcacg tcgacagtgg cgaggtggct
     1921 ggcgaacagc ggcgcggtga tgcggtactg gaaccgccgt tgcagcgagt cgttggtgac
     1981 gatcgtctcg ggcatcggga tgcccgaccc ggtggtgatg atgcgggtcg cggtgccgtc
     2041 gtcgccgtac gtcacggtgc agtcgacgat gccggggaac cagtggtgca acagctcggg
     2101 ccgcccgacc acgtcccaca catcggccgc cgagcggccg atcacgatgt cacggcgcag
     2161 cgtggccatc agcgcgaacc gccatccggg gccgcgcccg gcgtgggcgt ggcggcgatg
     2221 aagtcggcga tcacctgcgc cagcagcggc ccttgatcct cctgcaggaa gtgaccgcct
     2281 ccggcgatgg tggtgtgcgc ctgaccggcg gtgcccggca cgcggcgctg gaagacgccc
     2341 tcgccaccct tggtgatggg gtcgctgtca ctgaacgcgc acaggaacgg cttggtgaac
     2401 gactcgagca cggtccacgc ggcgaggttg tcggcgctgg ccggatcgtc gggcgcgctc
     2461 ggcaccagcg acggcagcac gcgggcaccg gccttgtagc tgtcgtcggg gaacggtgcg
     2521 tcgtaggccg cgatcacctc gggggtcagg tcggtgaccg tgccgccgtt gatgatgttg
     2581 ccgaccggga acttcggggc ggtggccgca aaacgctgcc aactgaggaa cgcctcgctg
     2641 agccgcgact cgccggtggg caggccggtg ttgccgacga cgacgcgggc gaagcggtcg
     2701 ggagcggcgg cgaccagccg caggccgatc aaaccacccc agtcctgaca gaacaaggtg
     2761 atgccggtca gatcgagatg agcaaacagc gcgtcgcgca tccaccccac ctggcgggcg
     2821 taggtgtaat cggactgctc cgccggcttg tcggatcgtc cgaaaccgac caggtcggga
     2881 gcgatcaccc gatgccccgc ggcgaccaag gtggggatca tgtgtcgata caagaacgcc
     2941 cacgacggct caccgtgcat caacagcact ggcgccgcgt cagacggacc ttcgtcgacg
     3001 tagtggacgc gcagcacagc gccgctgccg tcgcccgcgt cgacatcgac gtagtgcggt
     3061 gcgtatggga agtcggccaa cgcggcgaac cgctcgtccg gcgtgcgcag tacctgcatc
     3121 gtctgtcccc tcaatcgatg gccgttcaat cgatgaccgt tcgaacgatg gccgcgttga
     3181 acaacttttg tctgtccgag tgacagttta tctcacacgg gacgcgtgct gtccagcgct
     3241 gcaccgagac agcaccgctg caccgcttcg tcaggcgcgg gcgcgggtgc ggcgtagcag
     3301 cgggtcggcc gcccgcaaca caccaccggc catgcgcagc acgcggcccg cagcgccagg
     3361 tgtctgaccg tcgatgagat tgcccatgac ggacagggtg atgtcggctg ctcgttgggt
     3421 gccgaccgca atgcgcaaac ccgggcgcag cagccacggg tgaccgatga tgaagctgaa
     3481 ccgccgccct gtctgcagga agcggtcgaa gcgctcgccg acctcgcggt cgtagtgctg
     3541 cggagccgtg gccgggtcgg ccaggaacag gtcggcggcc agcgtgccgc tctccagccc
     3601 gtagtcgatc ccttcgccgt tcatcgggtt gacgaagccg gctgcgtctc cgatcgccaa
     3661 ccagccgtcg ccgtgacggc gcacgcagct catcggcaag cgccaagcgc ggggcttgtc
     3721 gaggtacgcg ccgagctgcc actcgtcgcg cacgagcgcc gcgtactggt cgagcaaggt
     3781 gttgaggttc aactgcttga accccttcat ggtcgacagc gcgccgaccc cgatgttgac
     3841 cgtgccgtca ccggccggga acatccaccc gtagccggga accggcgtgc cgtgctcgtc
     3901 gcgcaacgac aggcacgctt cgaggtggcg atcggcgtgg cgcggcgagg gcgcgtaggc
     3961 gcggatcgcg agcccgaacg gctcgtcggc aacccgctcg gcgcccaccc gcttggccgc
     4021 tgcaccctgc gcaccggcgg ccaacaccgt gagaggcgcg ccgaactgtt caccgtcagc
     4081 gcttacgccg acgacacgat cgccgtcgag ctcgggcaac gcctcggtat cgaaacggac
     4141 ctcgacgccc gccgcgatcg cgacatcgat caggtgcgtg tcgaagcgat gccgcggcca
     4201 caccgcgccg tggttgggaa agcgatccgt gacgggccag gccagctcgc ggcggcgctt
     4261 gcccgcgatc atccgcaatc cgtcgatgcg gtgcgcgggc gacaggtcga tggcgagttc
     4321 gttcagcgcg gcgactgccc gcggggtgag cccgtcgccg cacaccttgt cgcggccgta
     4381 cggacccttc tcgaacagcg tcacccgagc gccaccgcgc gccgcacgga tggctgccgc
     4441 tgatccagcc gggccaccac cgatgacgag caggtcacgc tgattcacgg gccgcagcct
     4501 gccagcattc gcgcccgacc ggacactgtg cccggccgac tccgcgtcca gtcgcgctga
     4561 ccggcggccc cggcggtggg gacattggtc actgtccacc gtgccacact gatcccatca
     4621 tggaatctat gaacgccann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4801 cccgccgcac ccgcgcatcg ccttcaccga agcgggtttc tccgaggtca accacctgta
     4861 cgtcgagaac gacccgcggt tggctctgtc gcaacctgcc agcttgttgg tcatcggccc
     4921 gcgcgggccg ggcctggcca aggcgctcca cctcggcagc accgccgagt ggctgatcac
     4981 ccggccaccg tcgccgatgc tgatcccccg ccatggccgc gtcacccgct cggtcaccgt
     5041 ctgccacgac ggctcttcac acgccacggt ggcgacccaa gcactggcgc gcatgccctg
     5101 gatcgagggc gtcgccgtca cggtgttggc cgtcgacgac agccgcagcg accccagcac
     5161 ggccgtcgag gaagcgaccg cgatcctcag cgacagcggg gccgtgctcg acgcccggat
     5221 gctggtcggc gacccgaccc aggtcatcct ccgtcacctc gaacgccacg gcaccgatct
     5281 gttggtgttc ggcacgagcg gcctgacggg actccagcgc ctccgcgtcg gctcgaccgc
     5341 atcggctctg gctcacgcga ctgacctgtc gatcctggtc gcctgcgacc acagcgtcta
     5401 cgaggcctga cgcccgccag ccctccaccg agcgaggcgc ctacccagcc cagagagtga
     5461 cggcgccgct ggcttcggcg gtgacgatca tcgaaccgtc ggggctgaac tcggcggcca
     5521 tcaccggcga cgtgccggcc aacaccgcga ggcgcggatc gccctcgccg cccagcaggg
     5581 tcgcgacatc ccacaccgcc gcggtgccat cggcgctggc ggtgacgatg cgaccgccgt
     5641 cggcgctgaa cgcacagctg aagatctctc cggtgtgccc tgcgatctgg aacggtgtct
     5701 ccgtcgtcag gtcccacacg tacagcgtcg agtccttgcc gcaggcgacg atcagggtgc
     5761 cgtcggggct gaactcggca tcgtcgacac cacccgcgat cgttccgaac gaaccaagtt
     5821 gcacgccgtc gaagttccac accagcacgg tgccgtcgtc ggccgccgtg aggaaccgct
     5881 cgccgtcgga cgcgaagtcg gccaactgca cgtcgccggt gtgaccggtg agccgcagcg
     5941 ggttggaccc gtcggtgagc ggccacacca acaccgtgtt gtcctggcca ccgccgacga
     6001 cgcgctgcag atcgttgctg aactgggcga agtacagccg ctcggtcgcg cccgtgtacc
     6061 ggacggcttc cgatcgcgtg ttcaggtccc acagccgggc cgtgccgtcc gcgcttgcgg
     6121 tcagcaacga tccgccgtcg ggcacgctgt actccgcctc gtagacgacg tcggtatgcc
     6181 cggtgagttc tgccagcagc gcgccgttgg cgacctccca ccaccgcacg acaccgtcgc
     6241 taccgacggt cgcggcgtgg ccgttgtcgg gaccgaacag agcgtcgtac acctcgccgc
     6301 catggccgtc gaggacatgg aaggtctcgc cggtgtcggt ggcccagatg cgcgccgtgc
     6361 cgtcggtgct cgacgtgatc agcagcgtgc cgtcgccgtt gaagtgcgcg gtgacgacgc
     6421 gcgcgttgtg acctgccaac tcatgaagga cctgatacga ctcggtgtcc ggcaggctgt
     6481 cggcggtgtt cggcgtgctc gggtcggtgg tgtcgggatc ggtggtgtcg ggatcggtgg
     6541 cgggggtcga accatccccg ccgccgatca caccgaggtt ttcgcattcg tgcgcaccga
     6601 tctcggccgc gagttgatcg agttcgttac tgaggtcgct gagttgtcgc tcggccgagt
     6661 cgatctcgtc ctgatcgtcg gcggcgcgcg cggcggccag atcgtcggtc gccccgatgg
     6721 tggtctcgac gttggtggtc accgcctgat gttgcgtttg caacaaatcg ggcggttgca
     6781 actcgtacag catccccgcc ccggcttcca acgcgttgat cacgcggccg aagtagtcgg
     6841 cgtcgtcggg atcgatgctg ccgatctcgc ccgcgacggc gcggcagagt tggtcgaggt
     6901 cggccacgta ggccgcctga tcgggcggca gcgattcggc ggtcggctcg ctggtggtgc
     6961 tgtcggtctc gtcggtctcg tcggtgcggt cggtcgaggg cccggcgtcg ccgttgttgt
     7021 cgtcgcgggt gagcacgaag atcgctccga cgacgagcat ggtcgaagca aactgcgccc
     7081 agagcgtctg gacctgcatc aggccgatgt tgtgcgtgcc ctcgggggcc gtgtcgaaca
     7141 tgacggctct ggccagcgac agcaggccga cgccggttag gacgagcgac gcggtcttca
     7201 aattgatagt cttcatagct tcctcatttc agttttggtt ggggttttgg cttgccttcg
     7261 ccttgggagg agctgatctc ctccctcaga tcaggtcccg gtacgcagca ggcgcgccga
     7321 ctc
//
LOCUS       DAORNF010000058         7256 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_7606_length_7256_cov_1.440356, whole genome shotgun sequence.
ACCESSION   DAORNF010000058 DAORNF010000000
VERSION     DAORNF010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 7256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7256
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7606_length_7256_cov_1.440356"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="PLV68_03570"
     CDS             complement(<1..348)
                     /locus_tag="PLV68_03570"
                     /inference="COORDINATES: protein motif:HMM:NF012457.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HRE00350.1"
                     /translation="MTIPEPPSSPLQRGVVVPASHVDRSRLIEDLSLLADAGADFVRI
                     GLDWPWMQPSAGGLDGDAVEFYLGAAQHARALGIDLWLTLTDRDVPHWFDDEGGFGDE
                     RFALHWWPRWVEHC"
     assembly_gap    357..366
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(389..940)
                     /locus_tag="PLV68_03575"
     CDS             complement(389..940)
                     /locus_tag="PLV68_03575"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRE00351.1"
                     /translation="MRVPRTFAFVDLSGFTNYTAAFGDDAAGRLLGAFRVIARDVASE
                     RGVRIAKWLGDGFMIVAINQIDCIAFAVDLGGKAAQVCSPLSLRVGIATGHALLFEGD
                     DYIGSAVNMASRLCDVAGHSEVLIPAMQLEGLPQGVEATSHGAFELRGFPGEIEVFNL
                     SGTVLDTDQRDASELWTRAPFGL"
     gene            1091..>1327
                     /locus_tag="PLV68_03580"
     CDS             1091..>1327
                     /locus_tag="PLV68_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00352.1"
                     /translation="MASRFTYSRWDGSQKGFELDADAVFEALTDDLLYHGDVNSALRR
                     MLQDGVRDRNGERMQGLREIMERLRRERQERLDQS"
     assembly_gap    1328..1394
                     /estimated_length=67
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1395..3110
                     /locus_tag="PLV68_03585"
     CDS             <1395..3110
                     /locus_tag="PLV68_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRE00353.1"
                     /translation="DNAMREATASGDERRAETARQSAQDRNLTLDFLPDDLAGKVREL
                     QQYDFESGEARQRFEELLDRLRQQLMQQAVDQISGDMQNMTPEDMARMKDMLAGLNDM
                     LDKRRAGQDPEFERFMEQFGDFFPENPATLDELLEQLAQRMAAMQAMLNSMTPEQRSQ
                     LQQLSDQLMDDMDLRWQMDQLGEHLRSMYPQQGWNRRYDFQGQDPLNLGQAMQTMQEL
                     AELDQLENLLRNASSPAMLAEADMDRVRDLLGDDAARSLERLSELAKMLEEAGLIQNK
                     EGRLELTPRGMRAIGSNALRELFSKLERDKTGQHQIERHGQGHERMYETKPYEYGDPF
                     NLDLQRTIRNALGRSTGTGDGSVRRIPVRLSPDDFEIERTEHLTKTSTVLLLDLSMSM
                     PMRDNFLPAKKVAMALHSLITSQYPRDYMGLVGFGELARTITPEQLPEVSWDFAYGTN
                     MHHALMLARQMLARQSGTKQIIMITDGEPTAHIRPDGSPEFNYPPTRETVETTLREVL
                     RCTKDDIRINTFMLDADRSLKSFIEKLTAINRGRAFFTTPDNLGDYVLLDFMEGKAAT
                     SRRSA"
     gene            3353..3637
                     /locus_tag="PLV68_03590"
     CDS             3353..3637
                     /locus_tag="PLV68_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HRE00354.1"
                     /translation="MSNEYEHISSSDDDRAWQDQANCLGVDPDLFFPERGASTREAKE
                     VCRGCVVRLDCLEFALVNGEKFGIWGGLSERERRRLRRQRAQAGRRTATA"
     gene            3654..>3820
                     /locus_tag="PLV68_03595"
     CDS             3654..>3820
                     /locus_tag="PLV68_03595"
                     /inference="COORDINATES: protein motif:HMM:NF014470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HRE00355.1"
                     /translation="MFPAFLDSPQEIIILVVVLVVLFGGSQIPKIAKNLGKAQTEFKK
                     ALDQGRGEGAAA"
     assembly_gap    3821..3858
                     /estimated_length=38
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3994..4266)
                     /locus_tag="PLV68_03600"
     CDS             complement(3994..4266)
                     /locus_tag="PLV68_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HRE00356.1"
                     /translation="MHDHTSEDAATQWMSQGNCRSYPPATFFPSDGVGVDRARKICAT
                     CPVAETCLEYALYNRIEHGVWGGCSERERRRILKRRRGLVVTALAS"
     gene            4532..4789
                     /locus_tag="PLV68_03605"
     CDS             4532..4789
                     /locus_tag="PLV68_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00357.1"
                     /translation="MSDVTIQPRAHAKIVYLGPVSPHWEVYAGYGDRTVLDEFRARVL
                     ARLVLLPRDDPQFRRNRERIIRDAEREGIVIEWDLGLIDDQ"
     gene            4888..>5402
                     /locus_tag="PLV68_03610"
     CDS             4888..>5402
                     /locus_tag="PLV68_03610"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440330.1"
                     /note="catalyzes the reversible transfer of the terminal
                     phosphate of ATP to form a long chain polyphosphate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HRE00358.1"
                     /translation="MVDERHLNRELSWLAFNRRVLSLAAEAGIPLLERVKFLAIFSSN
                     LDEFFQVRVAALHDQLAANIVTPTPDGRTPREQLAEISEVVRDLIADQEDLFDHELRP
                     LLQAQGIEILDWTDLTPSERSTAVDIYNQRILPVLTPLAVDPAHPFPYISNLTLNLAA
                     TVRDPETGERR"
     assembly_gap    5403..5506
                     /estimated_length=104
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5507..>5852
                     /locus_tag="PLV68_03615"
     CDS             <5507..>5852
                     /locus_tag="PLV68_03615"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440330.1"
                     /note="catalyzes the reversible transfer of the terminal
                     phosphate of ATP to form a long chain polyphosphate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HRE00359.1"
                     /translation="GMEIEEVAAFRITRNADLTLEDEEADDLLAAVELELRRRRFGKA
                     VRVEVHATMSSEMVDLVARELDLGADSVFTSRSLLDPTCLFALTGIDNPALKDVPWPP
                     VTAARIALAEEAD"
     assembly_gap    5853..5903
                     /estimated_length=51
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5904..>6135
                     /locus_tag="PLV68_03620"
     CDS             <5904..>6135
                     /locus_tag="PLV68_03620"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440330.1"
                     /note="catalyzes the reversible transfer of the terminal
                     phosphate of ATP to form a long chain polyphosphate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HRE00360.1"
                     /translation="SFASSVEAFLGEAADDPRVQTIKMTMYRTSGDSPIARSLIRAAE
                     RGVQVAVLVELQARFDEATNVTWAKALERAGVH"
     assembly_gap    6136..6255
                     /estimated_length=120
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6256..>6465
                     /locus_tag="PLV68_03625"
     CDS             <6256..>6465
                     /locus_tag="PLV68_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00361.1"
                     /translation="LYEDLGYITCDPAIGEDATQLFNHLTGYSRGEDYKALVVAPHTL
                     RKRITQLIENEARYGSEGRIVIKSN"
     assembly_gap    6466..6683
                     /estimated_length=218
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6684..6922
                     /locus_tag="PLV68_03630"
     CDS             <6684..6922
                     /locus_tag="PLV68_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00362.1"
                     /translation="ADMMPRNLDRRVETLVPVTHPRHQLWLDHVIALQLADDIVAFDL
                     QPDDTWQRVGPPGQFHPHSQERMYEWVATHHQRR"
     gene            complement(7025..>7256)
                     /locus_tag="PLV68_03635"
     CDS             complement(7025..>7256)
                     /locus_tag="PLV68_03635"
                     /inference="COORDINATES: protein motif:HMM:NF037170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRE00363.1"
                     /translation="LITWSGPPLMPRHGPDRIRFHVAPTHGTTRAVAIAHLLALGAHL
                     SPGHHTSHHASHHAAGHEVLTDPDANEFHLLP"
BASE COUNT         1190 a         2242 c         2183 g         1033 t
ORIGIN      
        1 acagtgctcg acccatcgcg gccaccagtg cagggcgaac cgctcgtcgc cgaagccgcc
       61 ctcgtcgtcg aaccaatgcg gcacgtcgcg gtcggtcagc gtcaaccaca ggtcgatccc
      121 gagggcgcgg gcgtgctggg cggcgccgag gtagaactcg accgcgtcac cgtcgagccc
      181 cccagcgctg ggttgcatcc acggccagtc gagcccgatg cgcacgaagt cggccccggc
      241 gtcggccagc aacgacaggt cttcgatcag ccgactccgg tcgacgtgac tggcgggcac
      301 cacaacgccg cgctgcaacg gcgacgacgg cggttcaggg atggtcacgg aaggttnnnn
      361 nnnnnngacc gagccagcgc gagatcgctc agagcccgaa cggggcgcga gtccacagct
      421 cgctggcatc gcgctgatcg gtgtcgagca cggtgccgct gaggttgaag acctcgatct
      481 cgccggggaa gccgcgcagt tcgaacgcgc cgtggctggt ggcctcgacc ccctgcggga
      541 gcccttcgag ttgcatcgcg gggatcaaca cttcgctgtg gcccgccacg tcgcacaacc
      601 gcgacgccat gttgacggcc gagccgatgt agtcgtcacc ttcgaacagc agggcgtggc
      661 cggtggcgat gccgacccgc agcgacagcg ggctgcacac ctgggccgcc ttgccgccga
      721 ggtcgacggc gaacgcgatg cagtcgatct ggttgatcgc cacgatcatg aagccgtcac
      781 ccagccactt ggcgatgcgc acgccgcgct cgctggcaac gtcgcgggcg atcacccgga
      841 acgcgccgag cagccgtccg gcggcgtcgt cgccgaaggc agcggtgtag ttcgtaaaac
      901 cggagaggtc gacgaaggcg aaggtgcggg ggacacgcat cgagacccag gataatcgat
      961 gttccgaacc ccgcagtcat caattcggcc agttcggtcg gccggttcgg tcggccggtt
     1021 tggtccgccg gtttgggcgg ccggttcggg cggccacagg tcactccggt acagccgcta
     1081 cggtacggac atggcgtcgc ggttcaccta ctcccgctgg gacggctccc agaagggctt
     1141 cgagctcgat gccgacgccg tgttcgaagc gctgaccgac gacctgctct atcacggcga
     1201 tgtcaacagc gccctgcggc gcatgctgca agacggggtg cgcgaccgca acggcgagcg
     1261 gatgcaaggc ctgcgcgaga tcatggagcg gctccgccgc gagcgccagg agcggctcga
     1321 ccagagcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnngacaac gcgatgcgcg aggcgacggc gagcggtgac gaacgccgcg
     1441 ccgagacggc ccgccagagc gcccaggacc gcaacctgac cctcgacttc ctgcccgacg
     1501 atctggccgg caaggtgcgc gagctgcagc agtacgactt cgagtcgggc gaggcgcgcc
     1561 agcgcttcga ggagctgctc gatcggctgc gccagcagtt gatgcaacag gcggtcgacc
     1621 agatcagtgg cgacatgcag aacatgacac ccgaggacat ggcgcgcatg aaggacatgc
     1681 tcgccgggct caacgacatg ctcgacaagc gtcgcgccgg ccaagacccc gagttcgagc
     1741 ggttcatgga gcagttcggc gacttcttcc ccgagaaccc cgccaccctc gacgaactgc
     1801 tcgagcaact ggcccagcgg atggcggcga tgcaggcgat gctcaactcg atgacgcccg
     1861 agcagcgctc gcagctgcaa cagctcagtg accaactcat ggacgacatg gacctgcgct
     1921 ggcagatgga ccaactcggt gagcacctcc gctcgatgta cccgcagcag ggctggaatc
     1981 ggcgctacga cttccagggc caggacccgc tcaacttggg ccaagcgatg cagaccatgc
     2041 aagagctcgc cgagctcgac cagttggaga acctgctccg caacgcgtcg tcgccggcga
     2101 tgctggccga ggccgacatg gaccgggtgc gcgacctgct cggtgacgac gcagcccgtt
     2161 cgctggagcg cctgtccgag ctggccaaga tgctcgaaga ggccgggttg atccagaaca
     2221 aggaaggccg tctggagctg acgccgcgcg gcatgcgcgc gatcggatcg aacgccttgc
     2281 gcgaactgtt ctccaagctc gagcgcgaca agaccgggca gcaccagatc gaacgtcacg
     2341 ggcagggtca cgagcggatg tacgagacca agccgtacga gtacggcgat ccgttcaacc
     2401 tcgatctgca acgcacgatc cgcaacgcgc tcgggcgctc gaccggcacc ggtgacggtt
     2461 cggtgcggcg catcccggtg cgactgtcgc ccgacgactt cgagatcgag cgcaccgagc
     2521 acctcaccaa gacgtcgacg gtgctgttgc tcgacctatc gatgagcatg ccgatgcgcg
     2581 acaacttcct gccggccaag aaggtggcca tggcgttgca ctcgctgatc acctcgcagt
     2641 acccacgcga ctacatgggt ttggtcggct tcggcgagct cgcccggacg atcacgcccg
     2701 agcagctacc cgaggtgagt tgggacttcg cctacggcac caacatgcac cacgcactga
     2761 tgctcgcccg ccagatgttg gcccgccaga gcggcacgaa gcagatcatc atgatcaccg
     2821 atggtgagcc cacggctcac atccgccccg acggctcgcc cgagttcaac tacccgccca
     2881 cccgcgagac ggtcgagacc acgctgcgcg aggtgctgcg ctgcaccaag gacgacatcc
     2941 gtatcaacac cttcatgctt gacgccgacc ggtcgttgaa gagcttcatc gagaagttga
     3001 cggcgatcaa ccgcggccgg gcgttcttca ccacgcccga caacctcggc gactacgtgc
     3061 tgctcgactt catggagggc aaggccgcga cgtcgcgtcg cagcgcctga cgcgctggag
     3121 atcggggccg gcgccagcgg cggctggagc gaatcgtccg cccagacgat cgccggcgag
     3181 cgaaaacacc aggaaatgtg gcctgcccct tgcaaagtgc ctggtcaatc agtgagaatg
     3241 acaccactgt aattacatgg gtgctgtcac caccgcgaac ggcagggttt gcggaccagc
     3301 aacaacatcg acggtgacca gcatcgcggc cgacaaaggc aggaggccac acatgagcaa
     3361 cgagtacgaa cacatctcct catccgacga cgaccgcgcg tggcaagacc aggccaactg
     3421 cctcggcgtc gaccctgacc tgttctttcc cgagcgcggc gcgagcacac gcgaagccaa
     3481 ggaagtctgc cgtgggtgcg tcgtgcgcct cgactgtctc gagttcgcgc tggtcaacgg
     3541 cgagaaattc ggcatctggg gtggcctcag cgaacgtgag cggcgccgcc ttcgccgcca
     3601 gcgggcccaa gctggtcggc gcaccgcgac tgcatagcgc tagagttcgg gccatgttcc
     3661 cggctttcct cgattcccct caggagatca tcatcttggt ggtcgtcctc gtcgtgctgt
     3721 tcggcggatc gcagattccc aagatcgcca agaacctggg caaggcccag acggagttca
     3781 agaaggcgct cgatcaggga cgcggcgaag gcgccgcagc nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnncc cgcctcgagc gcctcgaccg tgagcggagc gagtgacagc
     3901 accctgacgg gcgtcgtcac cgggatcgcg tttggttccg cgtgaacatc tacccgggcc
     3961 gctgggccgg gttcgttgct gagttcggct cggtcagctg gccagggccg tgacgaccag
     4021 gccgcggcgg cgcttgagga tgcggcggcg ctcacgctcg ctgcagccgc cccacacacc
     4081 gtgttcgatg cggttgtaga gggcgtactc caagcaggtc tcggccacgg gacaggtggc
     4141 gcagatcttg cgggcacggt cgacgccgac gccatcgctg gggaaaaacg tggccggggg
     4201 atagctgcgg cagttgccct gcgacatcca ctgggtcgcc gcgtcctcag atgtgtgatc
     4261 gtgcatcacg agcctttcgc tagttgccca cactgggtgg tcccacccgc tgcggccccg
     4321 aaggaccccg agccggccag ctccgtggtg cgtctctcct cagacgatcg acagagcggt
     4381 tcggttccaa tgcgagccag tcaaacacac gtcatctggg ctgccaatgc cgtgacccga
     4441 caatcaatct ttcggccgat acactgccca actcgctact gagcagggtt ttcgttgcct
     4501 gcgtacgagt ttcatcgcca ggagagttgt catgtccgac gtcaccatcc agccgcgggc
     4561 acacgccaag atcgtctacc tcggccccgt gtccccccat tgggaggtct atgccggcta
     4621 cggcgaccgc accgtgctcg acgagttccg ggcccgcgtg ctggcccgcc tggtgttgct
     4681 gcctcgcgac gacccccagt tccgccgcaa ccgcgagcgc atcatccgtg acgccgagcg
     4741 cgagggcatc gtcatcgagt gggacctcgg cctcatcgac gaccagtgac ctgaccagcg
     4801 tcagcaccag ttttcctggg tcggctctcg ccagaattcg cttcgtgttc accgtggcgc
     4861 cgacatggcg gcggctaact tgctggcgtg gtcgatgaac gccatctcaa ccgtgagctg
     4921 agttggcttg ccttcaaccg ccgcgtgctg tcgctggccg ccgaagctgg catccctctg
     4981 ctggagcggg tgaagttcct ggccatcttc tcgtccaacc tcgacgagtt cttccaggtg
     5041 cgcgtcgccg cgctgcacga ccaactcgcg gccaacatcg tcacgccgac cccggacggg
     5101 cgaacaccgc gtgaacagtt ggccgagatc agcgaggtgg tccgcgacct gatcgcggac
     5161 caggaggacc tgttcgacca cgagctccgt ccgttgttgc aagcgcaagg gatcgagatc
     5221 ctcgactgga ccgatctcac gcccagcgag cgcagtacgg cggtcgacat ctacaaccag
     5281 cgcatcttac ccgtgctgac gccgttggcg gtcgacccgg cgcacccctt tccgtacatc
     5341 tccaacctca ccctcaacct cgccgcgacc gtgcgcgacc ccgagaccgg tgagcgccgc
     5401 ttnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncggc atggagatcg
     5521 aagaggtcgc cgcgttccgc atcacccgca acgctgacct gaccctcgag gacgaggaag
     5581 ctgacgacct gctcgcagcc gtcgagctcg aactgcgccg ccgtcgcttc ggtaaggcgg
     5641 tgcgggtcga ggtccacgcc acgatgagct cggagatggt cgacctcgtc gctcgcgagc
     5701 tcgacctcgg tgccgactcg gtgttcacca gccgttcgct gctcgacccg acgtgtctgt
     5761 tcgcgctgac cggtatcgac aatcccgcgc tcaaggacgt gccgtggccg ccggtaaccg
     5821 cggcccgcat cgccctggcc gaagaggccg acnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5881 nnnnnnnnnn nnnnnnnnnn nnngtcgttc gccagttcgg tcgaagcgtt cctcggcgaa
     5941 gccgccgacg acccgcgggt gcagacgatc aagatgacga tgtaccgcac ctcgggtgac
     6001 agccccatcg cgcgcagcct catccgtgct gccgagcggg gtgtgcaggt ggccgtgttg
     6061 gtcgagttgc aagcccgctt cgacgaagcc accaacgtca cctgggccaa ggcgctggaa
     6121 cgtgcggggg tccatnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6241 nnnnnnnnnn nnnnnggctg tacgaggacc ttggctacat cacgtgcgac cccgcgatcg
     6301 gtgaggacgc gacccagctg ttcaaccacc tcacgggata cagccggggc gaggactaca
     6361 aggcactcgt cgtcgcgccg cacaccctgc gcaagcggat cacccagctg atcgagaacg
     6421 aggcccgcta cggcagcgag ggccggatcg tgatcaagag caactnnnnn nnnnnnnnnn
     6481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6661 nnnnnnnnnn nnnnnnnnnn nnncggccga catgatgccg cgcaacctcg accgtcgggt
     6721 cgagacgctg gtgccggtga ctcatccgcg ccaccagctg tggctcgatc atgtgatcgc
     6781 gctgcagctc gcggacgaca tcgtcgcctt cgacctgcaa cccgacgaca cctggcagcg
     6841 cgtcggaccg cccggtcagt tccaccctca cagccaagag cgcatgtacg agtgggtcgc
     6901 cacccaccac caacgccgct gacccatcgc tcaccggtgc cgacgcgtcg gtgtccgcga
     6961 tggctcgact ggggtcaggt tccaccgtcg gagcgggagg tggcgatcgt cgtagagcgg
     7021 tgctctacgg gaggaggtgg aactcgttgg cgtcggggtc ggtgagcacc tcgtggcctg
     7081 ctgcgtggtg gctggcgtgg tggctggtgt ggtggccggg ggacaggtgg gcaccgagag
     7141 ccagcaggtg ggcgatggcc acggcgcggg tggtgccgtg cgttggggcg acgtggaagc
     7201 ggatgcgatc ggggccgtgg cggggcatca gcggcgggcc gctccacgtg atgaga
//
LOCUS       DAORNF010000059         7250 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_7620_length_7250_cov_2.044892, whole genome shotgun sequence.
ACCESSION   DAORNF010000059 DAORNF010000000
VERSION     DAORNF010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 7250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7250
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7620_length_7250_cov_2.044892"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..760)
                     /locus_tag="PLV68_03640"
     CDS             complement(<1..760)
                     /locus_tag="PLV68_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRE00364.1"
                     /translation="MGFADARLHSEATGRALDGGSSGIGVDPQPDDVRSAVRDWLAEH
                     WSPDLDLYQWRSMLVDAGWAVPAWPRTWFGQELPDWSVPLVANEIDATGAVGIAVGGG
                     TTLAAPSILALGPDSLRHRFLHRTITGEVTWCQLFSEPGAGSDLAGLSTSAVLDGDRW
                     IVNGQKLWNTSAHHADYGLLLARTDPAAPKHAGITYFVLPMHQPGVEVRPLRQMNDHS
                     SFNEVFLTDAEVPVDLVIGEVGKGWTGALTTLAFE"
     assembly_gap    895..1005
                     /estimated_length=111
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1006..>1266)
                     /locus_tag="PLV68_03645"
     CDS             complement(<1006..>1266)
                     /locus_tag="PLV68_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00365.1"
                     /translation="PVLSAYVNVVCHPDRDTARRLVTSILSVFARFAVMHGTVTGPAD
                     TATMRTLHDVANAYDMTTHSRVGSAQTEALSPEFIDRYAIAGP"
     assembly_gap    1267..1311
                     /estimated_length=45
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1312..1953)
                     /locus_tag="PLV68_03650"
     CDS             complement(<1312..1953)
                     /locus_tag="PLV68_03650"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HRE00366.1"
                     /translation="MSEPNWSEDAPTQFWTSGMPIPRIAEQQAVTAEELGWDGIVFPD
                     SQNLAADCYSILTLAATATDRLGLGVGVTNTFTRHPAVTASAIATVQAISRGRAVLGI
                     GRGDSALAQIGLAPVGLSSFERDVAAIQTYLRGEPVSFDRSAARADGVHGVDQLEVAT
                     RPQESRIEWIRPTDGKVPVEIAASGPRVLGIAARHADIVSLNVGADPARVAWAV"
     gene            2133..3209
                     /locus_tag="PLV68_03655"
     CDS             2133..3209
                     /locus_tag="PLV68_03655"
                     /inference="COORDINATES: protein motif:HMM:NF013044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HRE00367.1"
                     /translation="MTATMHEQVNQAAARQLLYNLRHDTVQQEPAVLRVPTAHFTDPV
                     RAQAEMALFRTLPLVVGHHSELAEPGATMARQLLDTTVIVRRDHGDRIVGYQIAARET
                     PADIGRWTSEQLASLAAVFVEQHSGLVWVDFSNQRDRSVADYLGDEVLHQLNQFELDR
                     SVLFMHEQFDLDVNWKLVMDGACDILHLKFLHPNGVGKLLVTGRSVFVKHGRHGQNFT
                     PRKRLEVLARESEPSDDDLDQVWRYVSGQIRLYPNAMNIIAPDHIEFWTVLPTADPSR
                     CRVDIRFLVRPELLDDEMAARIRYSWEILREAAFTEDFPMEISIQRNAVAHPDSEFTY
                     GCNETSIRHLHDCLARDLNELDAR"
     gene            3206..4123
                     /locus_tag="PLV68_03660"
     CDS             3206..4123
                     /locus_tag="PLV68_03660"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HRE00368.1"
                     /translation="MTAPRPSLIFPTKEVAVEASAITAIATAAERCGYHRIVVSDHVL
                     GVSHDDRPKPLFGLYDHTTPFHEPLALLAYLAGITSTVELATGVLVLPQRQVAVVAKQ
                     ALQIDMMSGGRFVLGVGAGWNYVEYEALGADFESRGAMLDEQIGLLRTLWTEPLIDFS
                     GRFHHLDRVALAPLQDRRIPIWVGGYSPPALRRAAAVGDGFIFSGRSPATRAQAAQLQ
                     AQTRRRRGWSAEATLPVEVWLGYDDGPDVWAREIELWRPLGCVSVALRTSTAAGRQAT
                     PARPPRLELSEHLEAIEHFAEWFREQVGD"
     gene            4366..5112
                     /locus_tag="PLV68_03665"
     CDS             4366..5112
                     /locus_tag="PLV68_03665"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HRE00369.1"
                     /translation="MTDTILVERPQDGTALITLNRPEKLNALNISTFQRLDEVLTELE
                     ADDSVRVVVLTGTGKAFSAGYDVREMEGLDEAATLLDYQTREPWMFHLADLAKPVVAA
                     VNGLAYGGGALLASCADIRIGCAGSRFKITAVPYGGINGSWNFPLIVGWGKTKEWLLT
                     GRVIEPEEALASGLLNHIVPDDEVLPRALETAATIASYPPAAVQGLKSVLHANVGRSL
                     ADSYNGENTYLHTKIKFGRTSDLFSNFLNK"
     gene            5240..>5609
                     /locus_tag="PLV68_03670"
     CDS             5240..>5609
                     /locus_tag="PLV68_03670"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRE00370.1"
                     /translation="MMLAQLGADVILIEDPARPGPLDFSRRTYNRGKRSILLDLAGSD
                     ADRATFHELVAGADIVFTTDDRATLRERGMAPEQLEALNPGLVHVSITAFGLDGPKAD
                     YHATDLVALAAGGLLKITGDQ"
     assembly_gap    5610..5619
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5620..>7250
                     /locus_tag="PLV68_03675"
     CDS             <5620..>7250
                     /locus_tag="PLV68_03675"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRE00371.1"
                     /translation="PVRTTVPQGYLNAAANSAGAALVGLFERNESGRGQLIDVSAQAS
                     LLQSTMSMVLADPFRAPQASRMAGGYTVPPLDIKMVWTCQDGMVTVTFMFGLSVGFFT
                     KRLMEWVHEEGFCSDEMLATDWVRFSAQVQSGEETIEKYEQIKETLQTFLLTKTKAEL
                     LEASLERGLLIAPITTIADVADSPQLADRDFWEDVERADGTTERHCGPIAKFGATPLA
                     PLSPAPLPGADTIAIRREPRRHLSLRTPDVAPEAPLAGMKVLDFTWAMAGPAATRVLA
                     DYGATVIRVETAVKIEAARQLQPFLNAKIDNEGSGVFLNLNVNKLGVAINLADEASRD
                     VVHDLIRWADVVAESFTPGVMADWGYGYDAIRAVNPGVVMFQSALMGQTGPLRDFAGY
                     GNLSAAICGFYNVTGWPDRWPTGPYVAYTDYVSPWFTLPVLLAAFDHKRRTGEGQLID
                     FSQMEASLYMLGSAFTEYFETGAIVERQGNRDPQMAPHGVFPTSGDDEWIAIAIDSDE
                     AWASLCTIAGFDPALAELSVAQRLARAEEVEELLA"
BASE COUNT         1204 a         2282 c         2355 g         1243 t
ORIGIN      
        1 gctcgaacgc cagagtggtc agggccccgg tccaaccctt gccgacctcg ccgatgacca
       61 gatcgaccgg aacctctgcg tcggtgagga acacctcgtt gaacgagctg tgatcgttca
      121 tctgacgcag cggccgcacc tcgacgcccg gttggtgcat cggcagcacg aagtaggtga
      181 tgccggcgtg cttcggagca gccgggtcgg tgcgtgccag gagcagtccg tagtcggcgt
      241 gatgggcgct ggtgttccac agcttctgcc cgttgacgat ccaccggtct ccgtcgagca
      301 cggccgacgt cgacagcccg gcgaggtcag aacccgcccc cggttcgctg aagagctggc
      361 accacgtgac ttcgcccgtg atggtgcggt gcaggaatcg gtgccggagc gagtcggggc
      421 cgagggccag gatcgacggt gccgcgaggg tggtgccacc gccgacggcg atgccgaccg
      481 cgcctgtcgc gtcgatctcg ttggcgacga gcggcaccga ccagtcgggc aactcttggc
      541 cgaaccatgt ccgaggccac gcggggaccg cccagcccgc gtcgaccagc atcgaccgcc
      601 actggtacag gtcgaggtcc gggctccagt gctcggccag ccagtcgcgc acggctgacc
      661 gaacatcgtc aggttgggga tcgacgccga tgccggagct gccaccgtcc agtgcgcgcc
      721 ccgtggcctc gctgtgcagc ctggcgtcgg caaaccccat gggtgggacg ctaacgcgga
      781 cgcggtcaaa ttgtcgaccc aaaccattat gtgtttggtt agctccgcgc tgtgtggacg
      841 agcgggctca tgatcgaagc gcggggagca cctcgtgcgc gagttgggcc gcggnnnnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnggacc ggcgatcgcg
     1021 tagcggtcga tgaactccgg gctgagcgcc tcggtttgag ctgaaccgac ccgcgagtgg
     1081 gtggtcatgt cgtacgcgtt ggcgacgtcg tgcagggtgc gcatcgtggc tgtgtcagcg
     1141 ggcccggtga ccgtgccgtg catgaccgcg aaccgcgcga agaccgacaa gatcgacgtg
     1201 accagacggc gcgcggtgtc gcggtcgggg tgacacacca cattcacgta cgcgctcagc
     1261 accgggnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn naacggccca
     1321 ggcgacgcga gcggggtcgg caccgacgtt gagcgacacg atgtccgcgt ggcgggcggc
     1381 gatgccgagc acacgcggtc cgctcgccgc aatttcgacg ggcaccttgc cgtctgtcgg
     1441 tcgaatccat tcgatccggc tctcctgcgg acgcgtggcc acttcgagct ggtcgacgcc
     1501 gtgcacgcca tcggcgcgtg ctgcggatcg gtcgaaggac accggctcgc cgcgcagata
     1561 cgtctggatg gcggcgacgt cgcgctcgaa cgaggagagg ccgacgggcg cgagcccgat
     1621 ctgagccaac gccgaatctc cacgaccgat acccagcacc gctcggcccc gcgagatcgc
     1681 ttgcaccgtg gcgatcgccg atgcggtcac cgcggggtgg cgggtgaagg tgttggtgac
     1741 gccaacgccg agaccgagcc ggtcggtggc ggttgcggcc agggtgagga tggagtagca
     1801 gtcggccgcg aggttctgcg agtcgggaaa cacgatgcca tcccaaccga gttcctcggc
     1861 ggtgacggcc tgctgttcgg cgatgcgtgg gatcggcatg ccagaggtcc agaactgcgt
     1921 tggcgcgtcc tcgctccagt tcggttcgct catcgctcgg tggcccccat gcagcagaaa
     1981 aacctagcag tgtctgcgcg aaggatgggc tgctggtcga ccgactgtcg ccgagcggtc
     2041 ttgattggct ctgctggtgg agatacggtc cgatccgaag atcaactgtt gtcgaacggt
     2101 gttctgtttc ggcactgaga gagggtaagg ccgtgactgc gaccatgcac gaacaggtca
     2161 accaagcggc cgcgcggcaa ctcttgtaca acctgcgcca cgacacggtg cagcaggagc
     2221 cggcggtgct gcgcgtgccg accgcgcact tcaccgatcc agtgcgggcc caggctgaga
     2281 tggccctctt ccgcacgctg ccgttggtcg tcggccacca ctcggagctc gctgagcccg
     2341 gggcgacgat ggcgcgccag ctcttggata ccacggtgat cgttcgtcgc gatcatggcg
     2401 accgtatcgt cggctatcag atcgcggctc gcgagacgcc tgctgacatc gggcgatgga
     2461 cgagcgagca actcgccagc ttggccgctg tgttcgtcga gcaacactcc ggcttggtgt
     2521 gggtcgattt ctccaaccag cgtgatcggt cggtcgctga ctacctgggc gacgaggtac
     2581 tgcaccaact gaaccagttc gagctggacc gctcggtgct gttcatgcac gagcaattcg
     2641 acttggacgt gaactggaaa ctggtgatgg atggtgcttg cgacatcctc cacctcaagt
     2701 tccttcatcc caacggagtt ggaaagctcc tggtcaccgg ccgctcggtg ttcgtcaagc
     2761 atggacgaca cggtcagaac ttcacaccgc gcaagcggtt ggaggtgttg gcccgcgagt
     2821 ccgaaccgag cgacgacgat ctcgaccagg tgtggcgcta cgtgtccggc caaatccgtc
     2881 tgtatcccaa cgcgatgaac atcatcgccc ccgaccacat cgagttctgg acggtgttac
     2941 cgaccgccga cccgtcgcgg tgccgtgtcg acatccgatt cctggtccgt cccgagctac
     3001 tcgacgacga gatggctgcg cgcattcgct atagctggga gatcctgcgc gaagccgcct
     3061 tcaccgagga cttcccgatg gagatctcga tccagcgcaa cgcggttgcc cacccggaca
     3121 gcgagttcac gtacgggtgc aacgagacgt cgatccgcca cctgcacgat tgtctcgccc
     3181 gcgacctgaa cgagttggat gctcgatgac agcgccccgc ccgtcgctga tctttccgac
     3241 caaggaagtg gccgtcgaag cgagcgctat cacagcgatc gcgaccgcgg cggaacgctg
     3301 tggctaccac cgcatcgtcg tgtccgacca cgtgctcggg gtctcgcacg atgaccgccc
     3361 gaagccgctg ttcggtctct acgaccacac cacgccgttc catgagccgt tggcgttgtt
     3421 ggcgtatctc gctggcatca cctcgacggt cgagttggcg accggtgtgc tggtactgcc
     3481 tcaacgccag gtcgccgtcg tcgccaagca ggcgctgcag atcgacatga tgtcgggagg
     3541 gcgattcgtg ttgggggtcg gcgccgggtg gaactacgtc gaatacgaag ccctcggtgc
     3601 cgacttcgag tcgcgcggag cgatgctcga cgaacagatc ggattgttgc gcacgctgtg
     3661 gacagagccg ctgatcgact tctccggccg gttccaccat ctcgacaggg tggcgctggc
     3721 cccactccag gatcggagga tcccgatttg ggtcggcggg tattcgcccc cggcgctgcg
     3781 ccgagctgcc gcggtcggtg acgggttcat cttctccggc cgctcgccgg cgacgcgcgc
     3841 gcaggcagct caactccagg cgcagacgcg tcggcgacga ggttggagcg cagaagcgac
     3901 gttgccggtc gaggtgtggc tgggctacga cgacggtccg gacgtgtggg cccgcgagat
     3961 cgaactgtgg cggccgctcg gctgcgtgtc ggtcgccctg cgcacctcga ccgcggccgg
     4021 tcgccaggcc accccggcgc gtccaccgcg gttggagttg agtgagcatc tggaggcgat
     4081 cgaacacttc gccgagtggt ttcgcgagca ggtcggcgac tgaagcgttc gaggcaggct
     4141 cacccgcaac cgcctgtgat cgagcccggg acgcttccca ttgcatcatc tgccaaacag
     4201 tgctaggtta ttgcgatcgg tgcacgaggg gaaacgcgtc ggtcgaggag atcgcacgca
     4261 ccgattccct gcctcggcgt cgtcggtcct gattcgacga tgttcggttc ccggcgtgca
     4321 gcggacaagt catccttctg accccacgat cgaaagtgag ccatcgtgac cgacacgatt
     4381 cttgttgagc gcccgcaaga cggcactgcc ctgatcaccc tcaaccgtcc tgagaagctc
     4441 aacgcgctca atatctcgac gttccaacgt ctcgacgagg tgctgaccga gttggaggcc
     4501 gacgacagcg tgcgggtcgt ggtgttgacc ggcacgggca aggcgttcag cgccggctac
     4561 gacgtgcgcg agatggaagg tctcgacgaa gcggcgacgc tgctcgacta tcagacccgt
     4621 gagccgtgga tgttccacct ggccgacctt gccaagccgg tggtcgcagc ggtgaacggg
     4681 ctggcctacg gcggaggggc actcctcgcg tcgtgtgccg acattcgcat cggttgcgcc
     4741 gggtcgcggt tcaagatcac cgctgtgccc tatggcggta tcaacgggtc gtggaacttc
     4801 ccgctgatcg tcggctgggg caagaccaag gagtggctgt tgaccggacg ggtcatcgag
     4861 ccggaagaag cgcttgcatc gggcctgctg aaccacatcg tgcccgacga cgaggtgcta
     4921 ccgcgcgcgc tcgagacggc ggcgaccatc gcgagctatc cgccggcggc cgtgcagggg
     4981 ttgaagtcgg tgctccacgc caatgtcgga cgcagcttgg ccgacagcta caacggtgag
     5041 aacacctacc tgcacaccaa gatcaaattc ggccgtactt ctgatctgtt cagcaacttc
     5101 ttgaacaagt gacatcgatg cggttacgtg ttcgactgtg catcttcgaa tgatgtgatg
     5161 atccgactgg gaggatgacg atgaccctgc ttgctccata ccgtgtgctc gatctcaccg
     5221 acggggccaa ctggccgcga tgatgttggc ccaactgggc gccgacgtga tcttgatcga
     5281 agatccggcc cgccccgggc cgctcgactt ttcgcggcgg acgtacaacc gcggcaagcg
     5341 atcgatcctg ctcgatctcg ccggatccga tgccgaccgc gcgacgttcc acgagttggt
     5401 cgcgggagcc gacatcgtct tcaccaccga cgatcgagcc acgttgcgcg aacgaggcat
     5461 ggcccccgag caactcgagg cgttgaaccc ggggctggtg cacgtctcga tcaccgcctt
     5521 cggtctcgac ggccccaagg ccgactatca cgccaccgat ctggtcgcct tggcggccgg
     5581 tgggctgctg aagatcacgg gcgaccaggn nnnnnnnnnc gcccgtgcgc accaccgtgc
     5641 cgcagggcta cctcaacgcg gcggccaact cggccggtgc cgcgctggtc ggcctcttcg
     5701 aacgcaacga gtccggccgc ggtcaactga tcgacgtctc cgcccaggct tcgctgctgc
     5761 agtcgacgat gtcgatggtg ctcgccgatc ccttccgggc cccccaggcg tcgcgcatgg
     5821 ctggcggtta caccgtgccg ccgctcgata tcaagatggt ctggacgtgt caggacggca
     5881 tggtcaccgt cacgttcatg ttcggactct cggtcggctt cttcaccaag cgcctcatgg
     5941 agtgggtcca cgaggagggc ttttgcagcg acgaaatgct cgccaccgac tgggttcgat
     6001 tctcggccca ggtccaaagt ggtgaggaga cgatcgagaa gtacgagcag atcaaagaga
     6061 cgctgcagac cttcctgctc accaagacca aggccgagtt gctcgaagcc tcgctcgagc
     6121 gcggcctgct gatcgccccg atcacgacga tcgccgacgt cgccgattca ccgcaactcg
     6181 ccgaccgaga cttctgggaa gacgtcgagc gcgctgatgg caccacggag cgccactgcg
     6241 gcccgatcgc caagttcgga gcgaccccgc tggctccgct gtcgccagcg ccattgccgg
     6301 gggcagacac gatcgcgatc cgtcgtgaac cgcgtcggca cctgtcgctg cgtacgcctg
     6361 acgtggcacc cgaagcgccg ctcgcgggaa tgaaggtatt ggacttcacc tgggccatgg
     6421 ctggacctgc agcgacgcgg gtgttggccg actacggggc cacggtgatc agggtcgaga
     6481 ctgcggtgaa gatcgaagcc gcccgtcagc tgcagccatt cctcaatgcc aagatcgaca
     6541 acgagggcag cggcgtgttc ctcaacctca acgtcaacaa gctcggtgtt gcgatcaacc
     6601 tggccgacga ggcttcgcgc gacgtcgttc acgacctgat ccgctgggca gacgtcgtcg
     6661 ccgagtcgtt cacccccggt gtcatggccg actggggata cggctacgac gccattcgtg
     6721 cggtcaaccc gggcgtcgtc atgttccaga gcgccctgat gggtcagacc gggccactgc
     6781 gtgacttcgc tggttacggc aacctgtcgg ccgcgatctg cgggttctac aacgtcaccg
     6841 gctggcccga tcggtggccg accggaccgt acgtggctta caccgactac gtgtcgccct
     6901 ggttcacgct gccggtgctg ctcgccgcgt tcgatcacaa gcggcgcacg ggcgaaggtc
     6961 aactgatcga cttctcgcag atggaagcga gcctgtacat gctcggctcg gccttcaccg
     7021 agtacttcga gaccggcgcg atcgtcgagc gacagggcaa ccgcgatccg caaatggcac
     7081 cgcacggggt cttccccacg tcgggcgacg acgagtggat tgcgattgcg atcgacagcg
     7141 acgaggcgtg ggcatcgctg tgcaccattg ctggcttcga tcccgcgttg gctgagctct
     7201 ccgtggccca gcgcttggcg cgggccgagg aggtggagga gttgctcgcg
//
LOCUS       DAORNF010000060         7236 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_7645_length_7236_cov_1.951957, whole genome shotgun sequence.
ACCESSION   DAORNF010000060 DAORNF010000000
VERSION     DAORNF010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 7236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7236
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7645_length_7236_cov_1.951957"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>212
                     /locus_tag="PLV68_03680"
     CDS             <1..>212
                     /locus_tag="PLV68_03680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00372.1"
                     /translation="LAARRAITMRELGQSAEGIAATAVAYRLAVSAGDADAVGDVLLA
                     SRYPSTDSITDAGRHSEALARMWRGS"
     assembly_gap    213..280
                     /estimated_length=68
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <281..1174
                     /locus_tag="PLV68_03685"
     CDS             <281..1174
                     /locus_tag="PLV68_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00373.1"
                     /translation="GDGVARGQRVLSLCAELDDSRLTERLSPLLCYELFWDRSFVAVA
                     EGYAADCRARGEIIGALGGLSYVLGALMRRREITFEHPGFTELEVLGQSGGMWASLPA
                     ELMALCRGSLAADGAALGRLAAASLGTAAPALPLSRMVAEVHLRLLGTRAWQRLAATE
                     FVGDPRRWDVSNLVDLALFDAATATAAGDRSAAAGHLDTVAEPLLDAAPSVLTVARLP
                     VAALVLEALGDRDRAAQWLALNRAAVGNDLCLLPAVHLGPAESWLAMIADVAGDGDAA
                     LLHEQAARRLAALDARVLELA"
     gene            1354..>4275
                     /locus_tag="PLV68_03690"
     CDS             1354..>4275
                     /locus_tag="PLV68_03690"
                     /inference="COORDINATES: protein motif:HMM:TIGR01451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HRE00374.1"
                     /translation="MKDQDTFSARRWWRVLAAIAAIVATTATVVVVPVAPAAYAAGAA
                     TITKTLDDTQGAGYTAPDTFQAGALVRYRLRYQCSSLEEPCVTGTITDVLDPNLEYVS
                     VIPPSSTAVTVTPSFDVATNTLTVTLGSFDDDDSGDLVVVARVKSATPGGTEIPNQAS
                     IQVPGGPVVNTEVVTIRVPPSTPNWDLAKRTSSGVTTYAAGSQVSYDVGFSALTSIGN
                     VAVQSATLVDTYPAGAIVIDADGGVVDPVNHTITWVTGPAPVGTFPTRRVVLEFPADA
                     FPAGSSAVNHASVSWVFADGSTGTDTAQVGVNFNTAVPSTTLTKTGPSAAVTGTSVTY
                     NLRWRNDGNTPVVDPVITDVVPAGLTPVRLVRSDTWPAQAMHFEALVGGTWTEFASRV
                     AADPAWVPEGSVLTLPAGTTSVRIRVEGTLPAGASISVLLAAEVTAASGATVTNCLEA
                     TAALPGGGSLTATPACQPTQIVDPYAVLHTVKSTPNYADTAATSTRPGDEFTWSLATY
                     LDEGADISRLTVTDLLPPQLEYVSDECAILTSPNFIPYPSTLATINCSAGPTLTPTVT
                     TAGGQQRLTWDVDLNVTPMRNAYGGTTTYTSMSAGDDGRRVLVIKFRVRVKPGTAVGS
                     YTNEMASSSNEVPTTCSIRSAADAGDWDGDGSTTDVRCWATNTFIVDEAAVVAGEKWD
                     DGHPGLDHVRQDTGQPSAECPDWGGYTRYPCVAQTVPSGPFSYRWRLQNIGNVELTNY
                     VVYDVLPHVGDTGVSQLLSGEARGTDWQPNLTGPVVIESQPAGANATIEYNLSTDPCR
                     PELNQGASDGVWQTTCDDVWLTAAAVTDWSAVRSFRVRAFQQVDGIWPGWQPLGELVL
                     SAAMVAPANAPTSTSSPLNLSIAWNSMGTRAFRLNSSTQATLRILASEPRKVGIIIPF
                     VENLPDLVRLGNQVWRDTGAGANFDNGRYDTDESPIAGVTLELWRDVDGDGVFEPTGD
                     DS"
     assembly_gap    4276..4321
                     /estimated_length=46
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4322..5565
                     /locus_tag="PLV68_03695"
     CDS             <4322..5565
                     /locus_tag="PLV68_03695"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HRE00375.1"
                     /translation="MSESPYFVAIPAGQSALAAMASSTGATAGVIDNDDNGTPAPGYA
                     AVSDVRRVSVGGAPTGESDTNDSGSAETEADANVAVTADADSDLTIDFGFRPVGYDLA
                     LIKTRVTASPVQPGANVVWHVTVMNQGAVPSGDYTVTDTLPGGMGFVSASDGGTHAAG
                     VVTWNLTGLAPGASRVLTLTTRVDDVNKAPYRNWAEISADSGDDDDSTPDTNTGSDPD
                     GGLGTSPNDAVTNHNSTGFDSTGETPLPGGALDEDDNDYEDVDVAVVYDLALAKVTAA
                     DNVEVGNSITWTIRVQNQGNVPSGVYQITDRIPGGLTYVSCAGATSCVHAAGLVTYTM
                     PTLAPGAHADVTLVTTVADMNFQPFRNWAEISADGADAYDIPGVRDVEDKDSTPDTNT
                     GSDPSYGPALTATDNGGSNDA"
     gene            5656..>7236
                     /locus_tag="PLV68_03700"
     CDS             5656..>7236
                     /locus_tag="PLV68_03700"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00376.1"
                     /translation="MHYDLALIKTVDQTVVAEGGTVTWTITVKNQGNVDSGPYSVTDT
                     LPGGMSLVSSNPAGGQVGSVVTWTDRPNLTPGATATIVVVTRADDMTLKPFRNWAEIS
                     ADGADAYDLPGLDVEDEDSTPDTNTGADPGAGAGTPPNDAVDNHNDIDLDTPPNDEDD
                     NDYEEVGGDVVYDLALVKVVDNASPVQPGDTITWRLLVKNQGTVASHDFTVVDRIPTG
                     LAYGACTGGASCSQAGGVVTWTVTDLAPGGTTALTYTTTISDVTKAPFRNWAEISSDS
                     AADYDTTDKDSTPDADTGHDDTLPNDDYAPQQAADIDTDQAPGDEDDNDDAVVDVVIR
                     YDLALAKVTSTAVVNPTDQVTWTVRVRNQGNVASGAYTVTDTLPAGMVFVAASHGGTA
                     SGRVVTWTMPNLNPGQHVDVTVTTTISDPTLRPFRNWAEISSDSADTYDIPGVRDVED
                     VDSTPDANTGRDDTLPNDDYSGIDDLAVIGTDGQPAGDEDDNDDAVVDIAVRYDLALI
                     KQVQAGPFVVGDDVTWTIT"
BASE COUNT         1181 a         2580 c         2360 g         1001 t
ORIGIN      
        1 cctcgcggcg cggcgggcga tcacgatgcg cgagctcggg cagtccgcgg agggcatcgc
       61 ggccaccgct gttgcctaca ggctggcggt cagcgcggga gacgcggacg ctgtcggtga
      121 cgtgctgctc gcgtcgcgat atccgtcgac cgattcgatc accgatgccg gccgccactc
      181 cgaagcactg gcgcgaatgt ggcgtgggtc ggnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ggcgacggag tggctcgcgg
      301 ccagcgagtg ctgagcctgt gcgcggagct cgacgattcc cgtctcaccg agcggctgtc
      361 gccgttgctg tgctacgagc tgttctggga ccgctcgttc gtggcggtcg ccgagggcta
      421 cgccgctgac tgccgggcgc gcggcgagat catcggggcg ctcgggggtc tgagctacgt
      481 gctcggcgcc ctgatgaggc gccgcgagat caccttcgag catcctggct tcaccgagtt
      541 ggaggtgctc ggccagtccg gtgggatgtg ggccagtttg ccggcggagt tgatggccct
      601 gtgccggggc agcctcgcgg ccgacggtgc cgcactcggt cgtctggccg cggccagcct
      661 cggcaccgca gcgccggctt tgccgctgtc gcgcatggtg gccgaggttc acctgcgcct
      721 gctcggtact cgtgcctggc agcgactcgc cgcgaccgag ttcgtcggcg atccgcgccg
      781 gtgggatgtc tccaatctcg tcgacttggc gctgttcgac gcagcgacag cgacggccgc
      841 gggcgaccgg tccgccgctg caggtcacct cgacaccgtc gccgagccgc ttctcgacgc
      901 cgcgccgtcg gtactcaccg tggcccgcct gcccgtcgcc gcgctggtgc tggaggcact
      961 cggcgaccgt gatcgagcgg cccagtggct cgcgctcaat cgtgccgcgg tgggcaacga
     1021 cctgtgtctg ttgccggcgg tccatctcgg ccctgccgag tcgtggttgg cgatgatcgc
     1081 cgacgtcgcc ggcgacggtg acgcggcact gctgcacgaa caggcagcgc gccggttggc
     1141 cgccctggac gcgcgggtgc tcgaactggc ctgatcgcgg gagcggttct gtcgctccgg
     1201 cttaccgacg cgagaacgac gcaagcagga cgcagcgacg cctgagcgac gcaaaccggg
     1261 tctgatcggt cgcgagaggg gcgtgaatct ggcgcgcgag ttgacacttg ccagagccct
     1321 tgcgaacagg acacaagtgg gcgggagcaa ttcatgaagg accaggacac tttcagcgcg
     1381 cgccgttggt ggcgcgtgct cgccgcgatc gcggcaatcg tggcgacgac cgcgaccgtg
     1441 gtggtggtgc cggtggcacc cgccgcgtac gcggccgggg cggccacgat caccaagaca
     1501 ctcgacgaca cccagggggc cggctacacc gcgcccgaca ccttccaagc cggcgccctc
     1561 gtgcgctacc ggctgcggta ccagtgctcg tcgctggagg agccgtgcgt caccggcacg
     1621 atcaccgacg tcctcgaccc caacctcgag tacgtgtccg tgatcccacc gtcgagcacg
     1681 gccgtgaccg tgacaccgtc gttcgatgtg gcgaccaaca ccctgacggt caccttgggc
     1741 agcttcgacg acgacgactc gggcgacctg gtggtggtgg cgcgggtcaa gtccgccacg
     1801 ccgggtggca cggagatccc caaccaggcg agcatccagg tgcctggtgg tccggtcgtc
     1861 aacacagagg tggtcaccat ccgtgtgccg ccctcgaccc cgaactggga tctcgccaag
     1921 cgcacctcca gcggcgtcac cacctacgcg gctggttcgc aggtctccta cgacgtcggc
     1981 ttctcggcgc tgacatcgat cggcaacgtc gcggtgcaga gtgcgacgct ggtcgacacc
     2041 tacccggccg gcgcgatcgt gatcgacgcc gacggtggtg tggtcgaccc ggtcaaccac
     2101 acgatcacct gggtcaccgg tccggcgccc gtcgggacct tccccacccg ccgggtggtg
     2161 ctggagttcc cggccgacgc gttccccgct ggttccagcg ccgtcaacca cgcctcggtg
     2221 tcgtgggtgt ttgcagatgg aagtaccggt accgacaccg cacaggtcgg cgtcaacttc
     2281 aacacggcgg tcccgtcgac gacgctgacc aagaccggtc cgtccgccgc ggtcaccggc
     2341 acgtccgtga cctacaacct gcgctggcgg aacgacggca acacgccggt cgtcgacccg
     2401 gtgatcaccg atgtcgtgcc cgccgggctc acccccgtgc ggctggtgcg cagcgacacc
     2461 tggcccgcgc aggcgatgca cttcgaggcg ctcgtcggcg gcacgtggac cgagttcgcc
     2521 agccgcgtcg ccgccgaccc cgcctgggtc ccggaaggct cggtgctgac ccttcccgcc
     2581 gggaccacct cggtgcgtat ccgcgtcgag ggcaccctgc ccgccggggc gtcgatctcc
     2641 gtgttgctcg cagccgaagt caccgcggcc agcggtgcca ccgtcaccaa ctgcctcgag
     2701 gccacggccg ccttgccggg cgggggttcg ctcactgcga cgcccgcgtg tcagccgacc
     2761 cagatcgtgg acccctacgc cgtgctgcac accgtgaaga gcacgcccaa ctacgccgac
     2821 acggcggcga cctccacccg ccccggggac gagttcacct ggtccctggc cacctatctc
     2881 gacgagggtg ccgacatcag ccggctcacc gtcaccgacc tgttgccgcc gcagttggaa
     2941 tacgtctctg atgagtgtgc gatcctcacc tcgccaaact tcatccccta cccatcgact
     3001 ctcgccacga tcaactgcag tgcgggcccg accctgacgc ccaccgtcac gacggccggt
     3061 gggcagcagc ggctgacgtg ggatgtcgac ttgaacgtca ccccgatgcg caatgcctac
     3121 gggggcacca ccacctacac ctcgatgagc gccggcgacg atggccgccg cgttctcgtg
     3181 atcaagttcc gagtgcgggt caagcccggc accgccgttg gttcctacac caacgagatg
     3241 gcctcgtcct ccaacgaggt gcccacgacg tgctcgatcc gctcggcggc cgacgccggt
     3301 gactgggacg gcgacggcag caccaccgac gtgcgctgct gggccaccaa caccttcatc
     3361 gtcgacgagg ccgcagtggt ggccggtgag aaatgggatg acggccaccc tggcctcgac
     3421 catgtgcgcc aggacaccgg ccagcccagc gcggagtgtc ccgactgggg cggctacacc
     3481 cgctacccgt gtgtggccca gaccgtgccg tcgggcccgt tcagctaccg ctggcggttg
     3541 cagaacatcg gcaacgtcga actcaccaac tacgtcgtct acgacgtgtt gccccacgtc
     3601 ggcgacacgg gcgtgtccca actgctgtcc ggcgaagccc gcggcaccga ctggcagccg
     3661 aacctgaccg gaccggtcgt gatcgagtca cagcccgctg gcgcgaacgc gacgatcgag
     3721 tacaacctca gcaccgaccc gtgccgaccg gagctcaacc aaggggcgag cgacggcgtg
     3781 tggcagacca cgtgcgacga cgtgtggctg accgcagccg cggtcaccga ctggtcggcg
     3841 gtgcgcagct tccgggtgcg cgccttccaa caggtggacg gcatctggcc ggggtggcag
     3901 cccttgggcg aactcgtcct gtctgccgcg atggtcgcac ccgccaacgc gccgacctcg
     3961 acgtcgagcc cgctcaacct ctcgatcgcg tggaactcga tgggcacccg cgccttccgg
     4021 ctcaacagca gcacccaggc gaccctgcgc atactggcct ccgagccgcg caaggtgggc
     4081 atcatcatcc cgttcgtcga gaacctgccc gacctggtgc gcctcggcaa ccaggtgtgg
     4141 cgcgacaccg gcgcaggcgc caacttcgac aacgggcgct acgacaccga cgagagcccg
     4201 atcgcgggcg tgacgctgga gctgtggcgt gacgtcgacg gcgacggtgt gttcgagccg
     4261 accggtgacg acagcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4321 nacatgagcg aatcgccgta cttcgtcgcc atccccgccg gccagtccgc gctggcggcc
     4381 atggcctcgt ccaccggcgc gacggcgggc gtcatcgaca acgacgacaa cggcacgccc
     4441 gcgccgggct acgccgcggt cagcgacgtc cgacgggtga gcgtcggggg cgcgccgacc
     4501 ggcgagagcg acaccaacga cagtggctcg gccgagacag aggccgacgc gaacgttgcc
     4561 gtcaccgcgg acgccgactc ggatctgacg atcgacttcg ggttccgccc cgtcggctac
     4621 gacctggcgc tgatcaagac tcgggtcacg gcctcgccgg tgcagccggg cgccaatgtg
     4681 gtgtggcacg tcacggtgat gaaccagggc gcggtgccat cgggcgatta caccgtcacc
     4741 gacaccttgc cgggcgggat ggggttcgtc tcggccagcg acggcggcac acacgccgca
     4801 ggtgtggtga cctggaatct caccggtctc gcacccggcg cgtcgcgcgt gctcacgctg
     4861 accacgcgcg tcgacgacgt caacaaggct ccgtatcgca actgggccga gatctcggcc
     4921 gactccggtg acgacgacga ctcgaccccc gacaccaaca ccggctctga ccccgacggt
     4981 ggtctcggca cgtcacccaa cgatgcggtc accaaccaca acagcaccgg gttcgactcc
     5041 accggcgaga ccccgctgcc cggcggcgcg ctggacgagg acgacaacga ctacgaagac
     5101 gtcgacgtcg cggtggtgta cgacctggcc ttggccaagg tgacggcggc cgacaacgtc
     5161 gaggtcggca actcgatcac ctggacgatc cgggtgcaga accagggcaa cgtgccctct
     5221 ggcgtgtacc agatcaccga ccggattccg ggcgggctca cctacgtctc gtgtgccggg
     5281 gccacgtcgt gcgtccacgc ggcgggcttg gtgacctaca cgatgccgac gctcgcgccc
     5341 ggcgcccatg ccgacgtcac cctcgtcacg acggtggccg acatgaactt ccagccgttc
     5401 cgcaactggg ccgagatctc agccgacggc gccgacgcgt acgacatccc cggcgttcgc
     5461 gacgtggagg acaaggactc gacgcccgac accaacaccg gtagcgaccc gtcgtacggg
     5521 ccggcactga cggccacgga caacggcggc agcaacgacg cctaagtcgg catcaccgac
     5581 cttgccgtca tcgccaccga cggcgcgcca gccggtgacg aggacgacaa cgacgacgcc
     5641 atcgtccgcg gtgcagtgca ctacgacttg gcgttgatca agaccgtcga ccagacggtg
     5701 gtcgccgagg gcggcacggt cacctggacg atcaccgtca agaaccaggg caacgtcgac
     5761 tcgggtccct actcggtgac cgacaccctc cccggcggga tgagcctggt cagctcgaac
     5821 ccggccggag gccaggtcgg cagcgtggtc acctggaccg accgacccaa cctcacgccg
     5881 ggggcgaccg cgacgatcgt ggtggtcacc cgcgccgacg acatgacgct caagccgttc
     5941 cgcaactggg cggagatctc cgccgacggt gccgacgcct acgacctgcc cggcctcgac
     6001 gtggaggacg aggactccac tcccgacacc aacaccggcg cagaccccgg tgccggcgcg
     6061 ggcacgccac ccaacgacgc ggtcgacaac cacaacgaca tcgacctcga tacgccgccg
     6121 aatgacgagg acgacaacga ctacgaagaa gtcggcggcg acgtggtata cgacctggcg
     6181 ctggtgaagg tggtcgacaa cgcctcgccg gtgcagcccg gcgacacgat cacgtggcgg
     6241 ttgttggtga agaaccaggg cacggtggcc tcccacgact tcactgtcgt cgaccgcatc
     6301 ccgaccgggc tcgcctacgg cgcctgcacg ggcggagcca gctgcagcca agccggcggc
     6361 gtggtcacct ggaccgtgac cgacctggcg cccggcggca ccacggcgct cacctacacc
     6421 acgacgatca gcgacgtgac caaggcgccg ttccgcaact gggccgagat cagcagcgac
     6481 tccgcagccg actacgacac caccgacaag gactcgacgc ccgacgccga caccggccac
     6541 gacgacacgt tgccgaacga cgactacgcg ccccagcagg cggccgacat cgacaccgac
     6601 caggcgccgg gtgacgagga cgacaacgac gacgcagtgg ttgacgtggt gatccgctac
     6661 gacctggccc tggccaaggt gacctcgacc gcggtggtga acccgaccga tcaggtcacc
     6721 tggacggtgc gggtgcgcaa ccagggcaac gtcgcctcgg gtgcctacac cgtcaccgac
     6781 acgctgccgg cgggcatggt gttcgtcgcc gccagccacg gcggcacggc gtccggccgg
     6841 gtcgtcacct ggacgatgcc caacctgaac ccgggccaac acgtcgacgt cacggtgacg
     6901 acgacgatct cggacccgac cctgcgcccg ttccgcaact gggccgagat ctcctccgac
     6961 tcggccgaca cctacgacat ccccggtgtc cgtgacgtgg aagacgtcga ctccaccccg
     7021 gatgcgaaca ccggccgcga cgacacgctg cccaacgacg actactccgg catcgacgat
     7081 ctcgccgtga tcggcaccga cggccagcca gcgggcgacg aggacgacaa cgacgacgcc
     7141 gtggtcgaca tcgcggttcg ctacgacctg gccctgatca agcaggtgca agccggaccg
     7201 ttcgtggtcg gtgacgacgt gacctggacg atcacg
//
LOCUS       DAORNF010000061         7210 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_7684_length_7210_cov_1.840531, whole genome shotgun sequence.
ACCESSION   DAORNF010000061 DAORNF010000000
VERSION     DAORNF010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 7210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7210
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7684_length_7210_cov_1.840531"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="PLV68_03705"
     CDS             complement(<1..328)
                     /locus_tag="PLV68_03705"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRE00377.1"
                     /translation="MALDAVRDSQPDLVLLDLSLPDVDGLDVCRRLRAEVPAIPIVML
                     TARNEEVDIVVGLDAGADDYITKPFRVAELTARIRSRLRAANAATPTVPEADVFGSLR
                     IDRAARR"
     assembly_gap    404..465
                     /estimated_length=62
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(480..>1708)
                     /gene="hisD"
                     /locus_tag="PLV68_03710"
     CDS             complement(480..>1708)
                     /gene="hisD"
                     /locus_tag="PLV68_03710"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HRE00378.1"
                     /translation="ESIGRLLDDVRVNGDDAVCRALRDFDRISLEPHQLRVSEAELAG
                     AHVSSAVDAAIDAAIANLTTFNAAARERAGDWTIESQPGLFVGEKITPILSAGLFVPS
                     GKASYPSVAYQLGVPAVVAGVATIRLVVPPVPGGTGEVDPAVLVVCRKLGITEVFRVN
                     GPAGIAALGFGTESIGKVVKVVGPGSPAVTCAQVEMQRHGLATMMLLGPTESVVIADA
                     SADPARLAADLLIEAEHGTDSAVVLVTTSADLANAVDAVLADQLASLPGVRADAARAA
                     LGVNGGCVLVASLAEAADVVNCYAPEHLQVAVAPEAEAELVAGLTHAGEILIGQDTPF
                     SAANFLIGCPASLPTSGFAHVSSGITVEAFLKRTAVARASAGALAAMAPGIVALADHE
                     GFPAHAAAIRRRGLSD"
     assembly_gap    1709..1765
                     /estimated_length=57
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1950..>2105
                     /locus_tag="PLV68_03715"
     CDS             1950..>2105
                     /locus_tag="PLV68_03715"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF012912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HRE00379.1"
                     /translation="MPVTVVVGAQWGDEGKAKVIDLLAKEHGYVVRYQGGHNAGHTVV
                     VGDERYAL"
     assembly_gap    2106..2304
                     /estimated_length=199
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2305..>3063
                     /locus_tag="PLV68_03720"
     CDS             <2305..>3063
                     /locus_tag="PLV68_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HRE00380.1"
                     /translation="GTTLKGIGPAYADKARRVGLRAGDVLRAGTFAAAVRARAADESA
                     MLVAAGGEALDADAITEQFSALAARLAPYVTDTVSTLHDALAAGHQVLLEGAQATFLD
                     LDHGTYPFVTSSNPTAGGACAGTGLGPRTIDRVVGITKAYTTRVGAGPFPTELTDEIG
                     DKIVDIGREFGTVTGRRRRPGWIDCVMLRQAVRLNSLSELALTKLDVLDTFATVKVCT
                     GYRVGDTVLANYPDRIDLLAEVEPVYAELPGWNQS"
     assembly_gap    3064..3073
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3084..3212
                     /locus_tag="PLV68_03725"
     CDS             3084..3212
                     /locus_tag="PLV68_03725"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF012912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HRE00381.1"
                     /translation="MREPGQLPVEAQDFLCLVEREVGVPIRIVGVGAERDDYLLWS"
     assembly_gap    3391..3504
                     /estimated_length=114
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3505..>3749
                     /locus_tag="PLV68_03730"
     CDS             <3505..>3749
                     /locus_tag="PLV68_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HRE00382.1"
                     /translation="MPAGAWIHYGLTSSDVVDTAWCWMLRDACDELMTVLDDLLAVVT
                     ELAHTHRDTVMIGRTHGVHAEPTTFGVKAALWALQID"
     assembly_gap    3750..3772
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3773..>4153
                     /locus_tag="PLV68_03735"
     CDS             <3773..>4153
                     /locus_tag="PLV68_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HRE00383.1"
                     /translation="AVCKLSGAVGTYSNIDPRVEAFVGDTLGLTPVPATQVIARDRHA
                     EFLYACASIGSTMELIAVELRHLQRTEVRDAQEGFKPGQKGSSAMPHKRNPISAETIS
                     GLSRVLRGNLQAGMQDIALWHERD"
     assembly_gap    4154..4163
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4164..4549
                     /locus_tag="PLV68_03740"
     CDS             <4164..4549
                     /locus_tag="PLV68_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HRE00384.1"
                     /translation="HSSVERIVLPDSATLAHYVVSRMRRLLAGLQVYPDRMQANLDAS
                     HGLVFSQPVLLALVQSGLSRDEAYRIVQAAAMRAWDEGRSFRSLIEDDPDVAVEPTVL
                     DAAFDMQRSLGHIGTVFDALDAIER"
     gene            complement(4568..5110)
                     /locus_tag="PLV68_03745"
     CDS             complement(4568..5110)
                     /locus_tag="PLV68_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00385.1"
                     /translation="METAPKKPAKKAAGERAAVKKSAAQASSATIASSTKKEGATTMA
                     KRGPKSPMTSEHKAALAVGRAEGRIVRDYLTALRTNKPKRGRKRTPTSISTRLATIER
                     ELLDADPVAELRLVQERINLSAELASSGDVVDMAALEAEFVNVAKGYSERNGYSYAAW
                     REIGVSAATLSKAGISRGRA"
     assembly_gap    5281..5299
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5300..>5607
                     /locus_tag="PLV68_03750"
     CDS             <5300..>5607
                     /locus_tag="PLV68_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HRE00386.1"
                     /translation="QALRAAANTVDPSCHPHSLRAYFIRRGDHMAGVQYDVDRIRDGR
                     TFCTRRVVASQGGPAILNLEASFQHPEPSVTVDAIAMPRRIPSPDSLERRAWTPMIDC
                     "
     assembly_gap    5608..5664
                     /estimated_length=57
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5665..5999
                     /locus_tag="PLV68_03755"
     CDS             <5665..5999
                     /locus_tag="PLV68_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HRE00387.1"
                     /translation="MRATDSPGDRPIDHWCALAYLSDDVPSEAVLLARPSASFNIDNP
                     HDGVFTASLDHTIWFHRQLRADEWHLYDMSCLSFIAGRGLSFGYVFTADGTHVATVAQ
                     EVLVRESQ"
     gene            6010..6594
                     /gene="pyrE"
                     /locus_tag="PLV68_03760"
     CDS             6010..6594
                     /gene="pyrE"
                     /locus_tag="PLV68_03760"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HRE00388.1"
                     /translation="MSSLPDVTLPPLDRAREVLRHHVLTHSVKTGDFTLKSGAKSSWF
                     LDTKQTACRPDGIIAVADVALALIPAEATAIGGLTMGADPVAYAIAAVGATGGRGLRS
                     FSVRKEAKDHGVTGRIAGALQPGDKVVITEDTVTRGTSIFEAVDAVRAFGADPVLITV
                     IVDRGGTVAAMAAEAGIAYEPMLTAPDLGFAFGS"
     gene            complement(6605..6853)
                     /locus_tag="PLV68_03765"
     CDS             complement(6605..6853)
                     /locus_tag="PLV68_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00389.1"
                     /translation="MLHTHATPQAADTASTEPDTSPARPPTPVGRGTISVVLRVLTDR
                     AAAGHLVGQAEVVDTGEIVRVHDQDDLHRLIRRLAAGQ"
     gene            complement(6927..>7210)
                     /locus_tag="PLV68_03770"
     CDS             complement(6927..>7210)
                     /locus_tag="PLV68_03770"
                     /inference="COORDINATES: protein motif:HMM:NF015649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bacterial transcriptional activator
                     domain-containing protein"
                     /protein_id="HRE00390.1"
                     /translation="RRERDKRLFLSLVDLLAADAEQRGEYDEALRQLDLGIASEPMDE
                     SRYVRAGTLLLAQGRRATALEVVRRATAVLDEMGTTPGPDLARLVAELT"
BASE COUNT         1023 a         2240 c         2261 g         1135 t
ORIGIN      
        1 cccgccgcgc ggcgcggtcg atgcgcagcg agccgaacac gtcggcttcg ggcacggtcg
       61 gggtcgcggc gttggcggcg cgcaggcgag accggatgcg ggccgtcagt tcggccacac
      121 ggaacggctt ggtgatgtag tcgtcggcgc cggcgtcgag gccgaccacg atgtcgacct
      181 cttcgttgcg ggccgtgagc atcacgatcg ggatggccgg tacctcggcc cgcaggcggc
      241 ggcacacgtc gaggccgtcg acgtcgggca ggctgaggtc gagcagcacc aggtcgggct
      301 ggctgtcgcg gacggcatcg agggccagtt cacccagttc gatgtgctgg acactgaacc
      361 cttcggccta caacatccgc atcagcggtt ggctgatccg ttcnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncccgc gcaacgcgtt
      481 caatcgctca gaccgcgacg gcggatggcc gcggcgtggg cgggaaagcc ctcgtggtcg
      541 gccagggcga cgatgcccgg ggccatcgcc gcgagcgcgc cagcgctggc ccgggccacc
      601 gcggtgcgct tgagaaacgc ctcgacggtg atgcccgacg acacgtgggc gaagccgctg
      661 gtgggcagcg aggccggaca accgatcagg aagttggccg cgctgaacgg cgtgtcctgg
      721 ccgatcagga tctcacccgc gtgggtcagg ccggccacca actcggcctc ggcctcgggt
      781 gccaccgcga cctggaggtg ctcgggggcg tagcaattga cgacatccgc ggcttcggcc
      841 agcgaggcga ccagcacgca cccgccgttg acaccgagcg cggcgcgggc tgcgtcggcg
      901 cgcacgccgg gcaggctggc gagttgatcg gccaacacgg cgtcgaccgc gttggcgaga
      961 tcggccgagg tggtgaccag cacgaccgcc gagtcggtgc cgtgttcggc ttcgatcagc
     1021 aagtcggccg cgagacgggc cggatcagcc gaggcgtcgg cgatgacgac gctctcggtg
     1081 gggccgagca gcatcatggt ggccaggccg tggcgttgca tctcgacctg ggcgcaggtg
     1141 acggcaggcg agccggggcc gacgaccttg accaccttgc cgatcgactc ggtgccgaac
     1201 ccgagcgcgg cgatgccggc cgggccgttg acgcggaaca cctcggtgat gcccagcttg
     1261 cggcacacca ccagcactgc cgggtcgacc tcgcccgtgc ccccgggtac cgggggcacc
     1321 acgagccgga tcgtggcgac accggccacc accgcgggta caccgagttg gtaggcgacg
     1381 gacgggtagc tggccttgcc gctgggcacg aacaacccgg cgctgaggat cggggtgatc
     1441 ttctcgccga cgaacaaccc cggctgactc tcgatggtcc aatcgccggc tcgctcgcgc
     1501 gccgcggcgt tgaacgtggt gaggttggcg atcgcggcgt cgatcgcggc gtcgactgcc
     1561 gaggagacgt gcgcgccggc cagttcggcc tcgctgacgc gcagttggtg cggttccagg
     1621 ctgatccggt cgaagtcgcg cagtgcgcgg cacacggcgt cgtcgccgtt gacgcgcacg
     1681 tcgtcgagca gacgtccgat cgactcgcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnngcgcc catcgccgac cacgtcatcc tgcgcatcac
     1801 tggagcgttc gtcatgccca gcacggtatc cgcgtgctcc gcgggcgcga cctggcgatt
     1861 cattccgggt agggacgcct cggcggctag gatttcgtca cgttcccgag cgccccccgc
     1921 cgcgctcccc aatcgtgttg gagcgtgcca tgcctgtcac cgtcgtcgtc ggagcccagt
     1981 ggggtgacga aggcaaggcc aaggtcatcg acctactcgc caaagagcac gggtacgtcg
     2041 tgcgttatca aggcggccac aacgcgggcc acaccgtggt ggtcggcgac gagcgctacg
     2101 cgctgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnncggcac caccttgaaa ggcatcggtc ccgcgtacgc
     2341 cgacaaggcc cgccgggtcg ggctgcgtgc cggcgatgtg ttgcgagccg gcacgttcgc
     2401 cgccgcggta cgcgctcgcg cggccgacga gtcggcgatg ctcgttgcag ccggaggtga
     2461 ggcgctcgat gccgacgcga tcaccgagca gttcagtgcg ttggcggcca ggttggctcc
     2521 gtacgtcact gacacggtca gcaccctgca cgacgccctc gctgccggtc accaggtgct
     2581 gttggagggt gcccaggcca ccttcctcga tctcgaccat ggcacctatc cgttcgtcac
     2641 gtcgtccaac cccaccgccg gtggcgcgtg tgccggcacc gggctggggc cacgcaccat
     2701 cgaccgcgtg gtcggcatca ccaaggccta caccacccgc gtgggggcgg ggccgttccc
     2761 caccgagctc accgacgaga tcggcgacaa gatcgtcgac atcggccgcg agttcggcac
     2821 ggtcaccggg cggcgccgcc gtccgggttg gatcgactgc gtcatgctgc gccaggcggt
     2881 gcggctcaac tcgctgagcg agctcgccct caccaagctc gacgttctcg acaccttcgc
     2941 cacggtcaag gtctgcaccg gctaccgcgt gggcgacacg gtgctcgcca actaccccga
     3001 tcgcatcgat ctcctggccg aggtcgagcc ggtgtacgcc gaactgccag ggtggaacca
     3061 gtcnnnnnnn nnngctcgct gccgtgcgtg agcccggcca actgccggtc gaggcgcagg
     3121 acttcctctg cttggtcgag cgcgaggttg gcgtgcccat ccgcatcgtc ggcgtcggtg
     3181 ccgagcgcga cgactatctg ctctggtcct aggctgctct ggtcctaggc tgctctggtc
     3241 gtaggaggcc gcgatgatcc cccgttactc cctgcccgcg atgaccgcgg tgttcagcga
     3301 cgtcgaacgc ttccggcgct ggctggagat cgagctgttg gccaccgacg cccacgccga
     3361 actgggcctg gtgcccgcgg ccgatgcggc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnngcatgc ccgccggggc atggatccac tacggcctca
     3541 ccagcagcga cgtggtcgac acggcgtggt gctggatgct gcgcgatgcc tgcgacgagc
     3601 tcatgaccgt gctcgacgac ctgctcgccg tggtcaccga actagctcac actcaccgcg
     3661 acacggtgat gatcgggcgc acccacggcg tgcacgccga gccgacgacg ttcggggtca
     3721 aggccgcgct gtgggccttg cagatcgatn nnnnnnnnnn nnnnnnnnnn nnggcggtgt
     3781 gcaagctgag tggcgcggtc ggtacgtact ccaacatcga cccccgcgtc gaagccttcg
     3841 tcggcgacac gttgggtctc accccggtac cggcgacgca ggtgatcgcg cgcgatcgcc
     3901 acgccgagtt cctgtacgcc tgcgcctcga tcggttcgac catggagttg atcgcggtcg
     3961 aattgcgtca cctccaacgc accgaggtcc gcgacgcgca ggaaggcttc aagccgggcc
     4021 aaaagggcag ttcggccatg ccccacaagc gcaatccaat ctcagccgaa acgatcagtg
     4081 gtttgtcacg ggtactgcgc ggcaacctcc aagccggtat gcaagacatt gcactgtggc
     4141 acgagcgcga catnnnnnnn nnncccacag ttcggtcgaa cgaatcgtgc tacccgactc
     4201 ggcaacgttg gcgcactatg tggtgtcgcg gatgcgccga ttgttggccg gtttgcaggt
     4261 gtatcccgac cgaatgcagg caaacctcga cgccagccat ggattggtgt tcagtcaacc
     4321 cgtgctgttg gcgctggtgc agtcgggtct cagccgcgac gaggcctacc gcatcgtgca
     4381 ggctgcagcg atgcgggcct gggatgaagg gcggagcttc cgttcgctga ttgaggacga
     4441 ccctgatgtg gccgtcgagc caacggtgct cgatgcggcc ttcgacatgc aacgcagtct
     4501 cggccacatc ggcaccgtgt tcgatgcgct cgacgcgatc gagcgctgag tgatcaggcc
     4561 agcgtgatca ggcgcggccg cggctgatgc cggccttgct cagcgttgcg gcgctgacgc
     4621 cgatctcacg ccaggcggcg tagctgtatc cgttgcgctc gctgtaaccc ttggcgacgt
     4681 tgacgaactc ggcttcgagc gcggccatgt cgaccacatc acctgaactg gccagttcgg
     4741 cgctgaggtt gatgcgctct tgcaccaacc tcagttcggc cacgggatcg gcgtcgagca
     4801 gttcgcgttc gatggtggca agacgggtgg agatgctggt gggggtgcgc ttgcggccac
     4861 gcttgggctt gttggtgcgc aacgccgtca aatagtcgcg cacgatgcgg ccttcggcgc
     4921 gaccgacggc aagtgctgcc ttgtgttcgg acgtcatcgg actcttcgga ccacgcttgg
     4981 ccatggtggt tgctccttct ttcttggtgg acgaggcgat cgtcgcggac gaagcctgcg
     5041 cagcagattt cttcacggct gctcgttcgc ctgccgcctt cttggctggt ttcttgggtg
     5101 ctgtttccat agaggatatt tcgagatcac acagtatttc gggggatatg gtacaccgct
     5161 tcgtgtggtc agaccacgta acactacggt agcgatatgc gccttgcgga tatcctcacg
     5221 ttgacttcag cgggtcctga catctatatc ggcagcggtc cgcagtatcc atggggtggg
     5281 nnnnnnnnnn nnnnnnnnna caggcattgc gcgcggcagc caatacggtc gatcccagtt
     5341 gtcacccgca ttccttgcgg gcttatttca ttcgccgcgg cgatcacatg gccggtgtgc
     5401 aatacgacgt cgatcgcatt cgtgacgggc ggaccttctg cacccgtcgg gtggttgcca
     5461 gccagggcgg tccggcaatc ctcaatctgg aagcgtcgtt tcaacatccc gaaccgtcgg
     5521 tcaccgtcga cgccatcgcg atgccgcgcc gtatcccgtc gcccgattcg ttggaacggc
     5581 gcgcctggac gccgatgatc gactgtgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5641 nnnnnnnnnn nnnnnnnnnn nnnnggatgc gggccaccga cagtcccggc gatcgcccta
     5701 tcgaccactg gtgcgcgctg gcctacctgt ccgatgacgt gccgagcgag gcggtgctgc
     5761 tcgctcgacc ttcggcgtcg ttcaacatcg acaatcccca cgatggtgtg ttcaccgcca
     5821 gcctcgacca caccatctgg ttccaccgcc aattgcgcgc agacgagtgg cacctctacg
     5881 acatgtcgtg tctcagcttc atcgccgggc gcggtctgtc gttcggttac gtgttcacag
     5941 ccgacggcac ccatgtcgcg accgtggcgc aagaggtgct ggtccgcgag tcgcagtagc
     6001 gtcgccgtcg tgagctcttt gcccgatgtg acattgccgc cactcgacag agcgcgagag
     6061 gtactgcgcc accacgtctt gacccactcg gtcaagaccg gtgacttcac cctcaaatcg
     6121 ggcgcgaaga gctcgtggtt tctcgacacc aagcagaccg cctgtcggcc cgacggcatc
     6181 atcgctgtcg ccgatgtggc cctcgcgctg atccccgccg aggcgacggc aatcggcggg
     6241 ttgacgatgg gtgccgatcc cgtggcctat gccatcgcag cagtcggcgc caccggcggg
     6301 cgcggactgc gcagcttcag cgtgcgcaag gaggccaagg atcacggggt caccgggcgg
     6361 atcgccggag ccctccaacc cggtgacaag gtggtgatca ccgaagacac cgtcacccgt
     6421 ggaacctcga tcttcgaagc ggtcgatgcc gtgcgggcct tcggtgccga tccggtgttg
     6481 atcaccgtca tcgtcgaccg cggcggcacg gtcgcggcga tggcagccga ggcgggcatc
     6541 gcctacgagc cgatgttgac cgcccccgac ctcggcttcg cgttcggatc ctgatcggtc
     6601 gccatcactg cccggccgcg agccgccgga tcaagcggtg caggtcgtcc tggtcgtgca
     6661 cgcgcacgat ctcaccggtg tcgaccactt cggcttgacc gacgagatgc cccgcggcgg
     6721 cgcggtcggt cagcacccgc aacacgacac tgatcgtgcc ccggccgacc ggtgtcggcg
     6781 ggcgggcggg cgacgtgtcg ggctccgtcg aagctgtatc cgccgcttgt ggggtcgcgt
     6841 gagtgtgcag cacgggtgcc agcatcctcc gcgaccgtcc aacgaacgac caaccggcat
     6901 ccagcgcacg gttccttgat ggcccgtcag gtcaactcgg cgaccaggcg ggccaggtcg
     6961 gggccgggcg tggtgcccat ctcgtccagc accgccgtcg cccggcgcac gacttcgagc
     7021 gctgtcgcgc ggcgcccctg ggccaacagc agcgtgcccg cgcgcacgta gcggctctcg
     7081 tccatcggct cgctggcgat gccgaggtcg agttggcgca aggcctcgtc gtactcgcca
     7141 cgctgttccg catcggcggc gagcaggtcg accagcgaga ggaacaatcg cttgtcgcgc
     7201 tcgcggcggg
//
LOCUS       DAORNF010000062         7138 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_7785_length_7138_cov_1.594099, whole genome shotgun sequence.
ACCESSION   DAORNF010000062 DAORNF010000000
VERSION     DAORNF010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 7138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7138
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7785_length_7138_cov_1.594099"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            747..>1348
                     /locus_tag="PLV68_03775"
     CDS             747..>1348
                     /locus_tag="PLV68_03775"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRE00391.1"
                     /translation="MTNGADADLIIVAAKTDPTLGAKGISLFVVLDGAEGFAKGGKLD
                     KVGQPEATATELYFSDVFVADDDMLGEPGTGFAKMMRGLAQERLSASVSAVADAYAVF
                     ESTLEYVKERKAFGTPIGSFQYNKFQLATMATELDVTRAWVDRCVEAHLTGDLSAVDA
                     SKAKYWSTDVQNRVIDGCVQLFGGYGYMKEYRVARAWTDAR"
     assembly_gap    1349..1523
                     /estimated_length=175
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1708..2079
                     /locus_tag="PLV68_03780"
     CDS             1708..2079
                     /locus_tag="PLV68_03780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00392.1"
                     /translation="MIFGDRVQSPASSAAAVPVVQACRGAWWTVGWFVPEGHARCVQV
                     KAPDPDVEDWWEEYRALFAIVAAVGSAHTSTPARAWFGVWEGYGWDTSTTMYAFSETV
                     TGADRHRQAELRVQTMLGVTS"
     gene            2076..2372
                     /locus_tag="PLV68_03785"
     CDS             2076..2372
                     /locus_tag="PLV68_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00393.1"
                     /translation="MIRSGLRPVPRFELPNRAYYLLEGPVAAATELREPSDQSRWQRP
                     DLFWPDDRSWHVATDVDFWSLHVAGSEDFVADVVDAVPTSAVDVTLDASLIAED"
     gene            2642..3121
                     /locus_tag="PLV68_03790"
     CDS             2642..3121
                     /locus_tag="PLV68_03790"
                     /inference="COORDINATES: protein motif:HMM:NF022167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase"
                     /protein_id="HRE00394.1"
                     /translation="MTILDRLEGAGVTPWIDGGWGVDALLGRQTRAHGDLDIVIEQHQ
                     ETAARVVLAQEGFAEVPMWFSTAVHTVWHHDDGRALDLHVVVLDEEGGGVYGDEGVYP
                     AEGFEGRGTIGGRKVRCISAPVQIEFHRGYEERPQDRHDVLLLCQAFDLDIPNEYRN"
     gene            complement(3348..4388)
                     /locus_tag="PLV68_03795"
     CDS             complement(3348..4388)
                     /locus_tag="PLV68_03795"
                     /inference="COORDINATES: protein motif:HMM:NF018009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HRE00395.1"
                     /translation="MTEKSPALVEPPEGYTEWLAGLKSKVYAAQQRAALAVNTEMLQL
                     YWQIGREIRTRQEAQGWGRKITERLSQDLRSAFPDMKGFSRTNLDYMRSFADAWPEEA
                     ISQQAVGKLPWGHNLVLLTKLKSTADRLSYADAASRHGWSRNVLTIQIERRVLERQGK
                     AITNFDTTIPAPDSDLARESLKDPYRLDFLGLGEEAEERAVEDALIEHVTEFLLELGA
                     GFAFVGRQVHLEVGGDDFWLDLLFYQVKLHRYVVIELKADRFKPEHLGQLGFYMTAVD
                     RQLRDPATDGNTIGLLLCKSKNEVVAEYALEDNSKPLGIAEYRLVESLPEPLQTALPT
                     IEQIERELGTST"
     gene            complement(4431..>4630)
                     /locus_tag="PLV68_03800"
     CDS             complement(4431..>4630)
                     /locus_tag="PLV68_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00396.1"
                     /translation="TSSFPKALDTIFGAADVMSKYISEASDEIRSLTEFAVCALYEAK
                     DAVAEAHEVVSHLIFALDDQV"
     assembly_gap    4631..4659
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4757..>4979)
                     /locus_tag="PLV68_03805"
     CDS             complement(4757..>4979)
                     /locus_tag="PLV68_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017954219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRE00397.1"
                     /translation="RAKLPVISLHDLRHTHATILLKEGVPAKVVSERLGHANVAFTMS
                     VYQFILPGMQADAAHIFSNTVFGESQDIS"
     assembly_gap    4980..5016
                     /estimated_length=37
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5017..5910)
                     /locus_tag="PLV68_03810"
     CDS             complement(<5017..5910)
                     /locus_tag="PLV68_03810"
                     /inference="COORDINATES: protein motif:HMM:NF026010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HRE00398.1"
                     /translation="MRGHIVKRRSRYYAVVYEGVDPTTQKERHRWYQAGGTRKGAEKI
                     LADLVKRHHDGDYRAPDKITLGDYLIERWLPTKRAQLRFSTFDSYTRNVRNHVVPRIG
                     NIPLQKLTPEDLDQFYATLLTEGKLNGKKGGLSVKTVRYIHTIIRKALADAQRKGSVQ
                     RNVADLADAPKLSSAPRRPMKVWTAEQLRDFLDGITEHRMYPTIYLAASTGMRRGEVL
                     GIRWEDIDLDGLRLSVRNSVLNVAYAIEISEVKTDTSRRTVDLEPRTVGVLKAWRRTQ
                     LEERVALGIRPADDSLVFADPD"
     assembly_gap    6094..6233
                     /estimated_length=140
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6483..6526
                     /estimated_length=44
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6551..>6894
                     /locus_tag="PLV68_03815"
     CDS             6551..>6894
                     /locus_tag="PLV68_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00399.1"
                     /translation="MSNVLPATRPTRQREPWPDTITVVAWRDPVIEQAPGAIATTSEE
                     FLTWWAGTLGPSSVLVARHLAVYAADGEVEWPLDELGATFGLGRTQLGHTLDRLARFG
                     VIVRHGSTVAVR"
     assembly_gap    6895..6906
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6971..>7138
                     /locus_tag="PLV68_03820"
     CDS             6971..>7138
                     /locus_tag="PLV68_03820"
                     /inference="COORDINATES: protein motif:HMM:NF014520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HRE00400.1"
                     /translation="MRPDDDWQRFAACRGLPTPMFYPPTGASTGRALAVCARCPVRST
                     CEQHARSTGEQY"
BASE COUNT         1036 a         2130 c         2322 g         1213 t
ORIGIN      
        1 tgtgcccaac ggaacgcggc catacccacc tgcgaccaga gccgttcggc agatgccgac
       61 gcggcgcggt cgatgggtcc cgccggatcg acatggcggc cgcctttcgg gctttcgttg
      121 cgactgagtc ggccatgcga agatgcgttc cgtcggagtc ggctcagatc cgctcggcct
      181 cgggccgacg tgtggcagcg gttggctcgg acccagtgac gccgacggcc cccgccatcg
      241 gccccgcgat gcctgcccat gactgccagg agtgccgatg gaccgcaaga tcttcgaacc
      301 cgaacacgac gagttccgcg ccgcggtgcg ggccttcgtc gcccgcgagc tcacgccgtt
      361 cgccgagcag caccgggtcg agaagggctt cggccgcgat gtgtggcgca aggccggcga
      421 tgcgggcttc ctcgggttca tgatgccgtc ggagtacggc ggcgccgaca cggccgactt
      481 ccggttcaac acggtgttgg gggaggagtt ggcggcgctg ggctacgcct acgcgtcggc
      541 cttcggcatc aacgtcgacg tggtgtcgcc gtacctgcgc gatctcacga ccgacgccca
      601 gaaggcgcgg tggattccgg gcttttgctc gggcgagacg atctgcgcga tcgccatgac
      661 cgagcccggc accggctccg acctggccaa catcaagacc accgcgcacc gacggcggct
      721 ggctgctcaa cggctcgaag acgttcatca ccaacggcgc cgatgccgac ctgatcatcg
      781 tcgccgccaa gaccgacccg acgttgggcg ccaagggcat ctccctgttc gtcgtgctgg
      841 acggggccga ggggtttgcc aagggtggca agctcgacaa ggtcggccaa cccgaggcga
      901 ccgcgaccga gctgtacttc tccgacgtgt tcgtcgccga cgacgacatg ttgggcgagc
      961 cgggtacggg gttcgccaag atgatgcgcg gtttggccca ggagcgcctc tcggcctcgg
     1021 tgtctgccgt cgcggatgcc tacgcagtgt tcgaatcgac gctcgagtac gtgaaagagc
     1081 gcaaggcgtt cggcaccccg atcggttcgt tccagtacaa caagttccag ttggccacga
     1141 tggcgaccga gctcgacgtc accagagcgt gggtcgaccg ctgcgtcgag gcccacctca
     1201 ccggcgacct gtcggcggtc gacgcctcga aggcgaagta ctggtcgacc gatgtgcaga
     1261 accgggtcat cgacggctgc gtgcagctct tcggcggcta cggctacatg aaggagtacc
     1321 gcgtcgctcg cgcctggacc gacgcccgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnngcaggtt caaatcctgc ccccgccact caggaaagcc
     1561 ctggtagatc gcaagatcgg ccagggcttt ctccgttctg gaagtcccga agggctcgat
     1621 gtctaacgga atgtctaacg gacgttccgg aacgagtcgg gagaacccgg tgcggcgctc
     1681 gagttcgcgc ctgacagggt gacggtgatg atcttcgggg accgcgtcca atcgccggcg
     1741 tcgagcgctg ctgctgttcc ggtcgtgcag gcatgtcggg gcgcgtggtg gacggtgggc
     1801 tggttcgtgc ccgaggggca tgcgaggtgt gtgcaggtca aggcgccgga tccagatgtt
     1861 gaggattggt gggaggagta ccgggctctg ttcgcgatcg tcgctgcggt ggggtcggcg
     1921 catacgtcga cgccggcgcg ggcgtggttt ggggtctggg aggggtacgg ctgggacacc
     1981 agcacgacca tgtacgcctt ctcggagacg gtgaccgggg ctgaccggca ccggcaggct
     2041 gaactgcgag tgcagacgat gctcggcgtc acgagttgat ccggtctggg ttgcgtccgg
     2101 tgccgcgttt cgaactcccg aatcgcgcct actacttgct ggagggtccg gtcgccgcgg
     2161 ccaccgagtt gcgcgaaccg agcgaccaga gtcggtggca gcgaccggat ctcttctggc
     2221 ccgacgaccg cagttggcac gtcgcgaccg acgtcgactt ctggtcgctc cacgtcgctg
     2281 gttccgagga cttcgtggcc gacgtggtcg acgcggtgcc cacctcggcc gtcgacgtca
     2341 cgctcgacgc atctctgatc gccgaagact gacgccgccg aggcgcaccg ggagacgaga
     2401 tcatcgtttg cgccggctgg cgacggccct ccggtctgtg ccgagcggtg gccaacaagg
     2461 cgtgcccgtt acggccacgt cgccactctc gttgccacga tggcggaccc gcacagctct
     2521 gggatcgaca gcgaccggca cgtgggtgga ccaccctact gccgggtggc agccgagccc
     2581 gcgacgggtg ttcgttcgta tgcttgatcc ggtgcatacg catatgtcgg agttggaggt
     2641 gatgacgatc ctcgaccggc tcgagggtgc cggtgtgacc ccgtggatcg atggcggttg
     2701 gggcgttgac gcactgctgg gtcgacagac gcgggcccat ggcgatctcg acatcgtgat
     2761 tgaacagcat caggagactg cagctcgagt ggtgctcgcg caggagggct tcgctgaggt
     2821 tcccatgtgg ttctcgaccg cggtccacac ggtgtggcat cacgacgatg gacgggcgtt
     2881 ggatcttcac gtggtcgttc tggacgagga gggtgggggc gtgtacggcg atgaaggtgt
     2941 ctatccggcg gaaggattcg agggtcgggg cacgatcgga ggccggaagg tgcggtgtat
     3001 cagcgcgccc gttcagatcg agtttcaccg tggctacgag gaacgtccgc aggaccgcca
     3061 cgacgtgctg ctgctttgtc aggcgttcga cttggatata ccgaacgagt atcgaaactg
     3121 aatggacaag attgtgcggc gcgtgtcttg ctgaccacct gaccagatgt caccgagacg
     3181 ttgcgcgctg cggtacatct ccatctaccc cggctgtgtc ccccaccttc ggtcttgcgc
     3241 aggcgctcct cacgtggacc attcggcggc cgacaagcag atggcgtcgg ccgccaccat
     3301 gggcgcaacg cgcgccacgg acatcccacc agatctgagc ggcgtggtca ggtgctcgtg
     3361 ccgagttcgc gttcgatctg ctcgatcgtg ggcagggcgg tctggagcgg ctcgggaagc
     3421 gactcgacga ggcggtactc ggcgatgccg agcggcttgg agttgtcttc cagggcgtac
     3481 tcggcgacga cttcgttctt gctcttgcac agcagcaggc cgatggtgtt gccgtccgtc
     3541 gccggatcac ggagttgacg gtcgacggcg gtcatgtaga agccgagttg tccgaggtgc
     3601 tcgggcttga acctgtcggc cttgagttcg atgaccacgt agcggtgcag tttcacttgg
     3661 tagaagagca agtcgagcca gaagtcatca ccgccgacct ccaggtggac ttggcggccg
     3721 acgaacgcga acccggcacc caactccagc aggaactcgg tgacgtgctc gatgagcgca
     3781 tcctctacgg cacgctcctc ggcttcttcg cctaggccga ggaagtccaa gcggtacggg
     3841 tccttcagcg actcacgggc gaggtcggag tccggcgccg ggatcgtcgt gtcgaagttg
     3901 gtgatcgcct tgccctggcg ttcgagcacg cggcgctcga tctggatcgt gagtacgttg
     3961 cgcgaccagc cgtgccggga ggccgcgtcg gcgtagctca gccggtcagc tgtcgacttg
     4021 agtttggtga ggagtacgag gttgtggccc cacggcaatt tcccaacagc ctgttgggag
     4081 attgcttcct cgggccaggc gtcggcgaac gagcgcatgt agtcgagatt cgttcgcgag
     4141 aaccccttca tgtccgggaa cgcggagcga agatcttgcg acagccgctc ggtgatcttg
     4201 cgaccccagc cctgtgcttc ctgtcgggtg cggatctcgc gtccgatctg ccagtacagc
     4261 tgcaacatct cggtgttcac agcgagcgcg gcccgctgct gagcggcgta caccttgctc
     4321 ttcagcccgg cgagccactc ggtgtacccc tcgggcggct ccaccagcgc gggcgacttc
     4381 tcggtcatcg cctccatcct gttagggacg tccgtcattg gtccgggtgc tcacacctgg
     4441 tcgtcaaggg cgaagatcag gtgcgagacg acctcgtgcg cctccgccac tgcatccttg
     4501 gcctcgtaga gcgcgcacac cgcgaactcg gtgagtgacc ggatctcgtc cgacgcttcg
     4561 gagatgtatt tcgacatcac gtcggccgcg ccgaagatgg tgtccagcgc cttggggaag
     4621 gacgaggtca nnnnnnnnnn nnnnnnnnnn nnnnnnnnng ctccgtagat cgtcgagggg
     4681 tcgtcgcagc caggtcgggt gtgacaccag gagcggagct cttcgtacgc acggtcgaga
     4741 tgatcggttg ccgagttcat gagatgtcct gagattcgcc gaacaccgtg ttggagaaga
     4801 tgtgggcggc atcggcctgc atgcctggga ggatgaactg gtacacgctc atcgtgaacg
     4861 cgacgttggc gtgaccgagt cgttcgctga ccaccttcgc tgggacgcct tctttcaaca
     4921 ggatcgtggc gtgcgtgtgg cgcaggtcgt gcagcgagat cacgggcaac ttggccctgn
     4981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngtcc ggatcggcga acacgagcga
     5041 atcgtccgcc gggcggatgc cgagggcgac tcgttcttcg agttgggttc tacgccacgc
     5101 cttgagcacc cccacggtgc gcggctcgag gtcgacagtg cgacgtgagg tgtcggtctt
     5161 cacttctgag atctcgatcg cgtacgcgac gttgagcacc gagttgcgca cggacaagcg
     5221 cagcccatcg agatcgatat cttcccatcg gataccgagc acctcgccgc gtcgcattcc
     5281 ggtgctcgcg gcgaggtaga tcgttggata catgcggtgc tcggtgatgc cgtcgagaaa
     5341 gtcgcggagt tgctcggcgg tccacacctt catgggccgt cgcggcgccg acgagagctt
     5401 cggtgcgtcg gccagatcag caacgttgcg ttgcacgctc cccttgcgct gagcgtcggc
     5461 gagcgccttg cggatgatcg tgtggatgta gcgcacggtc ttgaccgaca gcccgccctt
     5521 cttgccgttg agcttccctt cggtcagcag cgtcgcgtag aactggtcga ggtcctcggg
     5581 cgtcagtttc tgaagtggga tgttcccgat gcgcgggacg acgtggttcc ggacgttgcg
     5641 ggtgtacgag tcgaacgtcg agaagcggag ctgggcgcgc ttggtcggca gccaccgttc
     5701 gatgaggtag tcgcccagcg tgatcttgtc gggggcgcgg taatcgccgt cgtgatgacg
     5761 cttgacgaga tcagcgagga tcttctcggc ccccttgcgc gtgccgcccg cctgatacca
     5821 gcggtgccgt tccttctgcg tcgtcgggtc gacgccttcg tacacgacgg cgtagtagcg
     5881 gcttcgccgc ttgacgatgt gtcctctcat gcgatgtcgg ctcctcgcgc gcgtcgccga
     5941 ccggtgtcgg cgttggtgag ccgctgcgca cgtgccctgt gcgctgcggc cggcggggga
     6001 cggaacgggg ccgcgtcccc gacggccaac cgcggaccgt agccatcggc gcgacgggca
     6061 actgttgtac tcacgggatc gccgtgaccg tggnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnccgccac
     6241 ctttacccag cgagctgacg gagcgaccgg gcgcgtccgc ggaccgcgcc cggccacgcg
     6301 ggtcggggtg tgccgtggcc cgcctgcggc gggtgtgccg tggcggtccg ccgccgagcc
     6361 ggcgtcaagc ccctcgcaag ctcggggccg gagcccttcg ggtggactcc ggctcggcgg
     6421 cggaccttgt gcccgtacgg gcaaggcccg gccaaccagt cgggctggcc cgctcggcgg
     6481 ggnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncgct cgacacgcaa
     6541 ggagcgcacc atgtccaacg tccttcccgc cacccgaccg acccggcagc gcgaaccatg
     6601 gcccgacacg atcaccgtcg tcgcgtggcg tgacccggtg atcgagcaag ctcccggcgc
     6661 gatcgcgacc acgagcgaag agttcttgac ctggtgggcg ggcacgctcg gtccgagctc
     6721 ggtgctcgtc gcccgacacc tggccgtgta cgccgccgat ggggaggtcg agtggccgct
     6781 cgacgagctc ggcgccacgt tcgggctcgg ccgcacccag ctcggccaca ccctcgaccg
     6841 gctcgcccgt ttcggtgtca tcgtccgcca cggctcgacc gtcgcggtgc ggatnnnnnn
     6901 nnnnnncagc cgccagcaga tcgaacgcct cccgcgctac ctggccgacg cgtaccggcg
     6961 gtgaaccgtc atgcgtcccg acgatgactg gcagcggttc gccgcctgcc gcggactacc
     7021 caccccgatg ttctacccac ccaccggcgc atcgacgggt cgagcacttg ccgtgtgcgc
     7081 ccgatgcccg gtccgctcaa cctgcgagca gcacgcgagg tcaacgggtg agcagtac
//
LOCUS       DAORNF010000063         7135 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_7790_length_7135_cov_1.767373, whole genome shotgun sequence.
ACCESSION   DAORNF010000063 DAORNF010000000
VERSION     DAORNF010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 7135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7135
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7790_length_7135_cov_1.767373"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(102..1826)
                     /locus_tag="PLV68_03825"
     CDS             complement(102..1826)
                     /locus_tag="PLV68_03825"
                     /inference="COORDINATES: protein motif:HMM:NF013557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00401.1"
                     /translation="MSDSPDNPPAGSAAETDTPEARSGQTGSGQPPDFASIIIQGGAL
                     QRIQAELAADTSAEIGGVLVGNAAPSTNFVLVTGSLPASRPLPTPGFEAVEALEADGV
                     GDAGESAVGDAGGDDSGSDFSTAPVTFSLSAEVLEDLARQVATVYPGQRIVGWYHSHP
                     GAGVFLAAHDVYIHTAFFAQPWQVNYVYDPVRHDGGFFGWSGRDLVRIPQWEVTSMSV
                     ATGAGLPVNEPALAAPAAAGGLAPTAGVARTSASGESPIGAGTSSLAASTAPRRRFSM
                     AAIVGAVVVLLAVIIGLVVLLGGGDDKASGPTSPVNTVDNDAGNNQTTSSATTVPDQG
                     SPMSSTSTSEPVVPQTNPPTNTETVVTDPPVLAFPATPTNVATPAARVGGDVAACTAG
                     SDGSFTPTSNCFVPLNNGNVLVFNGGRLRCVDPSAAADLATDVESFTVGVVDDPLPLV
                     ADGDLTPTCIDLSYVRQILANGAQTLDGLCGSSGTQINDGTRRCFAHNATSGAVVAIV
                     RGATDSTALEQSCTDGGDPVRAEIAWSSEVSTAWRLDSVVYRSATNDFVATASRRGVT
                     ATATLTCG"
     gene            2308..3573
                     /locus_tag="PLV68_03830"
     CDS             2308..3573
                     /locus_tag="PLV68_03830"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HRE00402.1"
                     /translation="MSSRIAIIGTGYVGLTTGACFAHLGHDVVCADIDPVRIERLSRG
                     ILPIYEPGLQEIVDAGIASGRLRFVVGAADAVRDCEFAYLCVQTPQGDDGSADLTYLQ
                     AAAAEIARALPSEAVVVNKSTVPVGSTKVVERVLGRSDVSVVSNPEFLREGSAVNDFL
                     KPDRVVIGSDSPAAATRVADLYGGTHGHVIVTDPASAETIKYAANAFLATKISFVNAV
                     AAVCEAVGADVNDVVLGIGYDKRIGHAFLNPGPGWGGSCFPKDTRAMVKIAEDAGYDF
                     NLLKGVVVVNDEQRERMVTKVERAVGDLDGAVVAAWGLTFKANTDDIRESPAIAVIAE
                     LQRRGAKVRAYDPQVPAHPSIDTAANPYDVCDGADALIVLTEWDEFRWLDLESVKARL
                     SRANIVDTRNLLDRGAAIRLGFSYQGVGR"
     gene            3573..>4363
                     /locus_tag="PLV68_03835"
     CDS             3573..>4363
                     /locus_tag="PLV68_03835"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HRE00403.1"
                     /translation="MQRVLLAGGAGFIGSHLADALIARGDEVIAVDNFISGRPENIDH
                     LLHHDRFLFLEQDVTDPLLIDGPLDAVMDLASPASPDDFATIPLEILAVGSTGTRNLL
                     DLAVIKHARFLLASTSEVYGDPLVHPQPESYWGNVSSIGPRSCYDEAKRFSEALAMAY
                     HRVHDLEIRIVRIFNTYGPRMRPDGRVVTNFIGQALHGEPLTLYGDGTQTRSFCYVSD
                     EVRGLLAALDGTITGPVNIGNPAECSMRHLAELVVELTGSASEIVT"
     assembly_gap    4364..4496
                     /estimated_length=133
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4546..>5488
                     /gene="galE"
                     /locus_tag="PLV68_03840"
     CDS             4546..>5488
                     /gene="galE"
                     /locus_tag="PLV68_03840"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HRE00404.1"
                     /translation="MTVLVTGGAGYIGSHTVRELRRLGRDVVVLDNLDTGFASTVLDA
                     PLVVGDIADEALVTSLCREHGVDSIVHFAAHKNVGESMQNPSKYWHNNVDGTVRLIDA
                     ALASGVGTIVFSSSCSVYGTPATVPVAETAPIRPESVYAETKAMIEKVLRWYGATHGL
                     ASVSLRYFNAAGASLDNRIGEDWTHSLNLVPVVMKAALGRRGPIEVFGTDYPTPDGTA
                     VRDYIHVEDLADAHVRALDYLADGGPTAAFNVGTGVGSSVTDIIRATEAASGRSVPVV
                     YGPRRTGDPIEVYADPTTVRDALGWQPRHGIDTIVATA"
     assembly_gap    5489..5544
                     /estimated_length=56
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5545..6557
                     /locus_tag="PLV68_03845"
     CDS             <5545..6557
                     /locus_tag="PLV68_03845"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRE00405.1"
                     /translation="PTITAIVVVHGSAAATVAAAVESLLASTGVDVFVVLVDNASPDG
                     GVAVEPWRRHPRVVVVTSARNDGFAAGVNQALARRRRGDLVWLLNDDATVEPDCLAHC
                     AATLRGAGARGAVVAAPRVMLASQPNLIDSLGVVLRRNGEAFNAFTGQRWTGQVADGE
                     TLTGACFAAGLFRADAFDANSVGRLDQRYRLYYEDIDWGLRAAARGLTAVASPDAVVH
                     HVHAGSTRTLGEPARYELVQRNLLLCAAKNLTWRSALRVWATRLAVHAKGTITGPMRA
                     RRWKAVAAALVGLVPVVVARRRQPHLSLAAEAALFARSEGHFPNIDTTTFLATPLATP
                     DD"
     gene            complement(6592..>6839)
                     /locus_tag="PLV68_03850"
     CDS             complement(6592..>6839)
                     /locus_tag="PLV68_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRE00406.1"
                     /translation="ELRRFRPVDAARRFAPRPLLVMHGEDDESVPVADARMLAEAHGS
                     AELRLLEGAGHRLRHDPRAVAILLGWLDRQRSAESEP"
     assembly_gap    6840..6858
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6859..>7135)
                     /locus_tag="PLV68_03855"
     CDS             complement(<6859..>7135)
                     /locus_tag="PLV68_03855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRE00407.1"
                     /translation="CGSSEGDFSLQSWVDDLRGAIDHLISEVQPSGVWLVGTNTGGSI
                     SICVAADDPRVRGAALLSPRADFDDWAAQPRRFLEHGREIGAIRTPGF"
BASE COUNT         1106 a         2481 c         2291 g         1049 t
ORIGIN      
        1 gtcacacgtc cagcgtgcat cagcacgtca cgtgtggcag gtcaactggg tcacgtcacg
       61 ccgacaagcc ggcgacccga cgctgatgcg gcaccgatac gtcagccaca ggtcagcgtt
      121 gcggtggcag tgacccctct gcggctggcc gtcgcgacga agtcgttggt cgcgctgcga
      181 tagacgaccg agtcgagtcg ccacgccgtg ctgacctcgc tcgaccacgc gatctcggcc
      241 cgcaccggat cgccgccgtc agtgcaggac tgctcgagcg cggtcgagtc ggtggcgccg
      301 cgcacgatcg cgacgaccgc gccagaggtg gcgttatggg cgaagcagcg acgggtcccg
      361 tcgttgatct gggtgccgga cgacccacac agcccgtcga gggtctgggc accgttggcc
      421 aagatctggc gcacgtagct gaggtcgatg caggtcgggg tgaggtcgcc atcggccacc
      481 agcggcagtg gatcgtcgac gacgccgacc gtgaacgact cgacgtcggt ggccaggtca
      541 gccgcagcgc tggggtcgac gcaacgcagc ctgccgccgt tgaagaccag cacgttgccg
      601 ttgttcagcg gcacgaagca gttcgatgtc ggggtgaagc tgccgtcgct gcccgcggtg
      661 caggccgcca cgtcaccgcc gacgcgcgct gctggcgtgg caacgttggt cggtgtagcc
      721 gggaaagcga ggaccggtgg atcggtgacg accgtctcgg tgttggtcgg cgggttggtc
      781 tggggcacca cgggttctga tgtggacgtg gacgacatcg gcgaaccctg gtcgggcacg
      841 gtggttgccg acgatgtggt ctggttgttg ccagcatcgt tgtcgacggt gttcacggga
      901 ctcgtcggcc ctgacgcctt gtcgtcgcca ccacccagca gcacgaccaa cccgatgatc
      961 accgccaaga ggaccaccac ggcaccgacg atggctgcca tcgagaagcg ccgccgaggg
     1021 gccgtgctcg ccgcgagcga cgatgtcccg gcaccgatcg gtgactcgcc ggaagcactc
     1081 gtccgggcca cgccagccgt cggtgcaaga ccgccagcag cggccggagc agccagcgcc
     1141 ggttcgttga ccggcaagcc cgcgccggtc gccaccgaca tcgaggtgac ctcccactgg
     1201 gggatccgga ccaggtcgcg gcctgaccag ccgaagaagc cgccgtcgtg gcgaacgggg
     1261 tcgtagacgt agttgacctg ccacggctgg gcgaagaacg ctgtgtggat gtagacgtcg
     1321 tgcgccgcca ggaatacgcc ggcgccggga tggctgtgat accacccgac gatccgctgc
     1381 ccgggataga ccgtggcgac ctggcgggcc aagtcctcca gcacctcggc cgacagcgag
     1441 aacgtgaccg gcgctgtcga gaagtcgctc cccgagtcgt caccacctgc atcaccaacg
     1501 gctgactcgc cagcgtcgcc aacaccgtcg gcctcgagcg cctcgacagc ttcgaaaccc
     1561 ggtgtcggca gcggacgtga cgccggcagc gagccggtga ccagcacgaa gttggtcgac
     1621 ggcgcagcgt tgccgaccag cacgccgccg atctcggccg acgtgtcggc tgccagctcg
     1681 gcctggatgc gctgcagcgc cccaccttgg atgatgatcg acgcaaaatc cggcggctga
     1741 ccggaaccgg tctggccaga acgagcctca ggtgtgtcgg tctcggcggc agagccggcc
     1801 ggtgggttgt ctggcgagtc gctcattcgg ggcgattgta ttctcaccgt caggttccgg
     1861 gcgtttcact ccccatcccc acgtcatgac caaccgtacg tcgaccgcgt tctaacccgc
     1921 catccaactc cccaggagtc gttccacgtg accagcaacg ccgcgtccag agcatgccct
     1981 cgctgccact ccgtggccag cggtgggcaa cgctactgcc gcgcctgtgg cacgaacctc
     2041 gtgacgggtg atccgtcgag cgagatcccc cgcccccgat gtcacgggca ctgatcgtga
     2101 tggtcgtcgc cgtctgtctg gcgctcggtg ccgccttggc cgtcgcgtgg cacgaggccc
     2161 gcgatgacgg tcagccaccg ccgccgaccg gcatgcacga accggtttcg cagaccgggc
     2221 ccggggacgt cacggttcac gccgcgatgg gccagatcgt ccgactcggg taggctgccc
     2281 ccgcctcgcc accggggtcg tcacaccatg agttcacgca tcgcaatcat cggcaccggc
     2341 tacgtcggcc tcaccacggg cgcctgcttc gctcacctcg gtcacgacgt cgtctgtgcc
     2401 gacatcgacc cggtacgcat cgagcggctg tcgcgcggca tcctgccgat ctacgaaccg
     2461 ggcctccaag aaatcgtcga cgccggaatc gccagcggcc gactgcggtt cgtggtcggt
     2521 gcggccgacg ccgtgcgcga ctgcgagttc gcctacctgt gcgtgcagac cccgcagggc
     2581 gacgacggct cggccgacct cacctatctc caagcagccg cggccgaaat cgcccgcgcc
     2641 ctgccgtctg aggccgtggt cgtcaacaag tcgaccgtgc cggtcggctc gaccaaggtc
     2701 gtcgagcggg tcctcggccg ctccgacgtc agcgtcgtct ccaaccccga gttcctgcgc
     2761 gaaggctcgg cggtcaacga cttcctcaag ccggaccgcg tggtgatcgg cagcgacagc
     2821 ccggctgcgg ccacgcgcgt cgccgacctg tacggcggca cccatggcca cgtgatcgtc
     2881 accgacccgg cctcggccga gacgatcaag tacgctgcca acgcgttctt ggccaccaag
     2941 atctccttcg tcaatgcggt ggcggcggtg tgcgaagccg tcggtgccga cgtcaacgat
     3001 gtcgtgctcg gcatcggcta cgacaaacgg atcggccacg ccttcctcaa ccctggcccg
     3061 ggctggggcg gctcgtgctt cccgaaagat acgcgggcga tggtgaagat tgcggaggac
     3121 gcggggtacg acttcaactt gctgaagggt gtcgtggtcg tcaacgacga gcagcgcgag
     3181 cggatggtga ccaaggtcga gcgggccgtc ggcgacctcg acggcgcggt ggttgcggcg
     3241 tggggcctga cgttcaaggc caacaccgac gacattcgcg aatcgcccgc gatcgccgtc
     3301 attgccgagc tccaacgccg cggcgcgaag gtgcgcgcct acgacccaca ggtgcccgcc
     3361 catccctcga tcgacaccgc cgccaacccg tacgacgtct gcgacggcgc cgacgcgttg
     3421 atcgtgctga ccgagtggga cgagttccgc tggctcgacc tggagtcggt caaggcccgc
     3481 ctgagccgcg ccaacatcgt cgacacccgc aatctgctcg accgcggcgc cgcgatccgg
     3541 ctcgggttca gctaccaggg cgtcggccgc tgatgcagcg tgtcctgctc gccggcggcg
     3601 ccggcttcat cggctcgcat ctcgccgatg cactgatcgc ccgcggcgac gaggtgatcg
     3661 ccgtcgacaa cttcatctct gggcgccccg agaacatcga ccacctgctc catcacgatc
     3721 ggttcctgtt cctggaacaa gacgtcaccg acccactgct gatcgacggt ccgctcgacg
     3781 ccgtgatgga cctggccagc ccagcgtcac ccgacgactt cgcgaccatt cccctcgaaa
     3841 tacttgcagt cggaagtacc ggcacgcgca acctgctcga cctggcggtg atcaaacacg
     3901 cccggttcct gctggccagc accagcgagg tctacggtga tccgctggtc cacccccagc
     3961 ccgagtcgta ctggggcaac gtcagctcga tcggcccgcg cagctgctac gacgaggcca
     4021 agcgattctc cgaagcactg gcgatggcct accaccgggt ccacgatctc gagatccgca
     4081 tcgtgcgcat cttcaacacc tacggcccac ggatgcgccc cgacggccgg gtcgtcacca
     4141 acttcatcgg ccaggcgctt cacggcgagc cgctgacgct gtacggcgac ggcacccaga
     4201 cgcgcagctt ttgctacgtg tccgacgagg tgcgcgggct gctcgccgcg ctcgacggca
     4261 ccatcaccgg cccggtcaac atcggcaacc ccgccgagtg cagcatgcgc cacctggccg
     4321 agttggtcgt cgaactcacc gggtcggcca gcgagatcgt cacnnnnnnn nnnnnnnnnn
     4381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngcac
     4501 cgaactgcac tccccaccgc cctccgaact gcgaggctga ccagcatgac cgtcctcgtc
     4561 acaggcggtg ccggctacat cggctcgcac accgtgcgcg aactgcgccg gctcggccgc
     4621 gacgtcgtgg tgctcgacaa cctcgacacc ggctttgcgt ccaccgtgct cgacgctccc
     4681 ctcgtcgtgg gcgacatcgc cgacgaagcg ttggtcacgt cgttgtgccg tgaacacggc
     4741 gtcgattcga tcgtgcactt cgccgcgcac aagaatgtcg gcgagtcgat gcagaacccg
     4801 tcgaagtact ggcacaacaa cgtcgacggc acggtgcgcc tgatcgacgc cgcgctcgcc
     4861 tccggggtgg gcacgatcgt gttctcgtcg tcgtgcagcg tgtacggcac acccgccacg
     4921 gtgcccgtcg cagagacggc gccgatccga cccgaaagcg tgtacgccga gaccaaggcg
     4981 atgatcgaga aggtgctccg ctggtacggc gccacccacg gcctggcctc ggtcagcctg
     5041 cgctacttca acgccgctgg ggccagcctc gacaaccgca tcggtgagga ttggacgcac
     5101 agcctcaacc tggttccggt ggtgatgaag gctgcgctcg gccgccgcgg accgatcgaa
     5161 gtgttcggca ccgactaccc cactcctgac ggcacagcgg tccgcgacta catccacgtc
     5221 gaggacctgg ccgatgccca cgtgcgggcg ctcgactacc ttgccgacgg cggaccgacc
     5281 gcggcgttca acgtcggcac gggcgtcggt agcagcgtca ccgacatcat ccgcgccacc
     5341 gaagcagcca gcggacgctc cgtgcccgtc gtctacggac cccgccgcac cggcgatccg
     5401 atcgaggtct acgccgatcc gaccacggtg cgcgacgccc tgggctggca gccccgccac
     5461 ggcatcgaca ccatcgtcgc caccgcctnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5521 nnnnnnnnnn nnnnnnnnnn nnnncgccga cgatcaccgc gatcgtcgtg gtccacgggt
     5581 cagccgcagc gaccgtcgcc gcggcggtcg agtcgctgtt ggcctcgacc ggcgtcgacg
     5641 tgttcgtcgt gctcgtcgac aacgcctcgc ccgacggagg ggtcgcggtc gaaccgtggc
     5701 gtcgccatcc gcgggtcgtc gtcgtaacca gtgctcgcaa cgacggcttc gccgccgggg
     5761 tcaatcaggc actcgcccgc cgtcgccgcg gcgatctcgt ctggctgctg aatgacgatg
     5821 ccaccgtcga gcccgactgc ctggcgcact gcgccgccac gctgcgcggt gccggtgcac
     5881 gcggcgccgt cgtggcggcg ccgcgcgtga tgctggccag ccagcccaac ctgatcgact
     5941 cgctcggggt ggtcctgcgc cgcaacggtg aggcgttcaa tgccttcacg gggcagcgct
     6001 ggacaggcca ggtcgccgac ggcgagaccc tgaccggggc ctgcttcgcg gccgggctgt
     6061 tccgtgccga cgcgttcgat gccaactcgg tgggtcgcct cgaccagcgc taccggctgt
     6121 actacgaaga catcgactgg ggtctgcgcg ccgcggcccg cggcctcacc gcagtggcca
     6181 gccccgacgc agtcgtccac cacgtgcatg cgggctcgac ccgcaccttg ggcgaaccgg
     6241 cgcgctacga actcgtgcag cgcaacctgt tgctgtgcgc ggccaagaat ctgacctggc
     6301 gctcggcgct gcgggtgtgg gcgacccggc tggccgtgca cgcgaagggc accatcaccg
     6361 ggccgatgcg ggcacgacgc tggaaggccg tcgcagcagc gttggtcgga ctcgtgccgg
     6421 tggtggtcgc tcggcggcgc cagccacatc tgagcctggc ggcagaagcg gcgctgttcg
     6481 cgcgcagcga gggccacttc cccaacatcg acacgacgac ctttctggcc acgcccctcg
     6541 ccacgccaga cgactgacgg ctccggccgg cacgactggt ggcggtgatc gtcagggctc
     6601 ggactctgcc gagcgttgac gatcgagcca accgagcagg atcgcgacgg cgcgcggatc
     6661 gtggcgcaag cgatggcccg caccttccag caggcgcaac tcggcgctcc cgtgcgcttc
     6721 ggccagcatc cgcgcgtcgg ccacaggcac gctctcgtcg tcttcaccgt gcatcaccag
     6781 caacggacgg ggggcaaagc ggcgggctgc gtcgaccggg cggaagcgac gcagctcgcn
     6841 nnnnnnnnnn nnnnnnnnaa agcccggcgt gcggatggcc ccgatctctc gcccgtgttc
     6901 gaggaaccgg cgcggttggg cggcccagtc gtcgaagtcg gcgcggggcg acagcaaggc
     6961 cgcgccgcgc acgcgcgggt cgtcggcggc gacgcagatc gagatcgacc caccggtgtt
     7021 ggtacccacc agccacacgc cagatggttg gacctcgctg atgaggtggt cgatcgcacc
     7081 gcgcaggtcg tcgacccacg actgcagcga gaagtcaccc tcgctactgc cgcag
//
LOCUS       DAORNF010000064         7123 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_7807_length_7123_cov_1.611488, whole genome shotgun sequence.
ACCESSION   DAORNF010000064 DAORNF010000000
VERSION     DAORNF010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 7123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7123
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7807_length_7123_cov_1.611488"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="PLV68_03860"
     CDS             <1..192
                     /locus_tag="PLV68_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HRE00408.1"
                     /translation="VEGSDVPAGYDSEFTNKNATIMSWNLMHTAKLLKDRGGFPVGGN
                     VAATWQDSTNAADQDPDRF"
     gene            complement(205..789)
                     /locus_tag="PLV68_03865"
     CDS             complement(205..789)
                     /locus_tag="PLV68_03865"
                     /inference="COORDINATES: protein motif:HMM:TIGR03086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03086 family metal-binding protein"
                     /protein_id="HRE00409.1"
                     /translation="MELIGALDLAVNEFARRLEFVSADQWALPTPCDEWVVHYLVAHV
                     VGGNRFARAVLDGFGAEDAIAAVMSSSQLGDDPIGVWATTVARQDEAFRRAGALDARI
                     DHPIGQISGRDLLGFRVFDITLHAWALARALGVDDTLRDALVDVVIAIIENGPAGMGF
                     GIQARRTNATASHLDRLLAISGRSASRSAPRSTT"
     gene            931..>1308
                     /locus_tag="PLV68_03870"
     CDS             931..>1308
                     /locus_tag="PLV68_03870"
                     /inference="COORDINATES: protein motif:HMM:TIGR03086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HRE00410.1"
                     /translation="MSSEVVDRTTRLIRNFDERVQAAPPDSWSNQAPCEEWTARDVVT
                     HVGNNLFGIAAGLGSGDPRQITPEEDIVSAWDEARQRFLGVLPSADLTTNLPGPFGPM
                     PAEELIGRLIATDVLVHTWDLARA"
     assembly_gap    1309..1318
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1696..>2335)
                     /locus_tag="PLV68_03875"
     CDS             complement(1696..>2335)
                     /locus_tag="PLV68_03875"
                     /inference="COORDINATES: protein motif:HMM:NF025687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HRE00411.1"
                     /translation="SSASLKLGALLEFGLIVTQLGTALVIHSLVKRQSETLSLSYIAA
                     RTMESVFAAIGIISIISVVNVANALSAGGDATALTVAGNSHASMYEWAFQWGPGLVAG
                     IGNGLILGYLMYRSRLVPRAMSILGLIGGPALVISELVPKRMAMIGLIGGPVLILSHV
                     LILCGAYKNGEGPSGLLTLPEAAWELSLGIYCAWKGFRPESPIARATEAADR"
     assembly_gap    2336..2443
                     /estimated_length=108
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2723..2789
                     /estimated_length=67
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2975..4417)
                     /locus_tag="PLV68_03880"
     CDS             complement(2975..4417)
                     /locus_tag="PLV68_03880"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HRE00412.1"
                     /translation="MATGDTTGVEALLQSVEASLHRTDPSPIVFDAELFEHLAAQLLI
                     QRAGLALLGGDLDGTSRFAAQALALVEPADHFRRGSATALIALARWAGGDLDTAVRYY
                     TEAISALVTADHVPDMLGCSLALADIQIAQGKLSDATRTFESGLRWTTEHPGLRGAAD
                     MHAGLSEVLIERNDLDAAASHLETSNELGESAGLPQHAYRWRVTMARLLRAYGDIDGA
                     LELIDQAAPLYDTDFSPPVRPVAAIRARVQLAQGDLRSALAWVSERGLTTDDELSYVH
                     EYEHITLTQILIAQHGKTPSTSALDAAIGMLDRLLVAAENGGRTGTAIEVLILRATAY
                     DAHGDTSAATEALADALVRAELEGHIRLFLHAGLSVTSLLRSVSSGDDATAYARRVLA
                     AIEDTAASPPSEPAKDTRRVGRGAFVDELSSRELDVLRLLRSELSGPEIARELHVSMN
                     TLRTHTKNVYTKLGATSRREATRRASEHGL"
     gene            complement(4542..5594)
                     /locus_tag="PLV68_03885"
     CDS             complement(4542..5594)
                     /locus_tag="PLV68_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00413.1"
                     /translation="MAEHQPALTLISAPAGFGKTTLLTEWFGNAADADRRSAWLALDA
                     ADNEPAVFGAYVVAALRSAASDVGATAESLLHGSQPLQAVTASLINDLEWLDVDVALV
                     LDDYHVIDSADIHDAICFLVEHAPPRFHLVLATRADPPLPLARWRARRDLLEFRAADL
                     RFTTEEAASYFTDSMHLQLSAVEVGALEARTEGWIAALQLAALSLRDRDDVGAFIDNF
                     AGDDRFVVDYLVEEVLDRQPDTVRSFLLHTSVLDRLTGGLCDAVTRGTDGRATLEQLD
                     RSNLFLVALDDRRHWYRYHHLFADVLHARLIHEDPDLIDELHRRAGAWYSAHGEPAKA
                     IEHAMAGHHIEEAAPD"
     gene            complement(5850..>7123)
                     /locus_tag="PLV68_03890"
     CDS             complement(5850..>7123)
                     /locus_tag="PLV68_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRE00414.1"
                     /translation="VVARRVLAATGLIDILPDVEGVGEHWGGDVIHCPFCHGYEVRDK
                     RIIQIVTHPMGLHPAGLFRQLTAQLTLVLHAGVSPDDPGLAPLRAAGVTVTDTPVRRL
                     VTGGDGHVCAVELTNGDQVDADAVVVGAPFRVRAEPFASLGLTPVPHPTGLGDFIETD
                     ATGETSVPGLYAAGNLTDPSQQVLQAAANGSRVGGMISFSLVAEDMANAARPSGNQAD
                     WEHRYGGGQIWSGNPNGTLVKEVEAAVPGRALDVGAGEGGDAVWLAEHGWDVTASDIS
                     QRALARIAAIATERGLQIGYHHADANGLHAYPAASFDLVSAHYAAIPRTPDDRGITNL
                     LAAVAIGGTLLVVGHDLEPMRHPIDISTHSQRFDPDAYVRVDHIAEAIASSSEWEIEI
                     NEKRPRPPGAASTHHVDDIVLRARRVGPRTN"
BASE COUNT         1114 a         2128 c         2485 g         1211 t
ORIGIN      
        1 gtcgagggca gcgacgtgcc ggcgggctac gacagcgagt tcaccaacaa gaacgccacg
       61 atcatgagct ggaacctgat gcacaccgcc aagctgctca aagatcgagg cggcttcccc
      121 gtcggcggca acgtcgccgc cacctggcaa gactcgacca atgccgccga tcaggatccc
      181 gaccgcttct gacgcccgac ccctctacgt ggttgaacgc ggcgccgacc gtgaagcgct
      241 cctgccgctg atcgcgagca accgatccag atgtgacgca gtggcattcg tccggcgtgc
      301 ttggattccg aagcccatgc cggcaggtcc gttctcgatg atcgcgatga cgacgtcgac
      361 cagggcgtcc cgcaaggtgt cgtcgacgcc gagcgctctg gcgagagccc aggcgtggag
      421 cgtgatgtcg aagacgcgga acccgagcag atctcgtcca gagatctggc cgatcggatg
      481 atcgatgcgg gcatcgagcg cgccggcgcg tcggaacgcc tcgtcctggc gtgccacggt
      541 cgtcgcccat actccgatgg gatcgtcgcc gagttgggat gatgacatca ccgcagcgat
      601 cgcgtcctcg gcgccgaatc cgtcgagcac cgctcgggcg aaccggtttc ccccgacgac
      661 atgcgcgacc agatagtgga caacccattc atcgcacgga gtcggcaacg cccactgatc
      721 cgccgacacg aactcgagcc gacgggcgaa ctcgttcacg gcgaggtcga gcgcaccgat
      781 cagttccaca gacagacatc ctcacgcgct cgcgcacctg atcaccgatc gaacggcgac
      841 tccgttgtca gttcggctga tcgagaagga acgacaaacc gcctcggcag ctgcccgggc
      901 cctccctcgg tcgcgggtag cgtcggcacc atgagcagcg aagtggtcga ccgcacaaca
      961 cgcctgatcc gcaacttcga cgagcgcgtc caagcggccc cgcccgacag ttggtccaac
     1021 caggcgccct gcgaggagtg gacagcgcgc gacgtcgtca cccatgtcgg caacaacctg
     1081 ttcggcatcg cagccggtct cggcagcggc gacccgcgcc agatcacacc ggaagaggac
     1141 atcgtgtccg cctgggacga agcacgccaa cggttcctcg gcgtcctccc atcagctgac
     1201 ctcaccacca acctgcccgg accgttcggg ccgatgcccg ccgaagaact gatcggtcgc
     1261 ctcatcgcca ccgatgttct cgtccacacg tgggacctgg ctcgcgccnn nnnnnnnntc
     1321 ggcggggacg agcatctcga cgaagcggcg atcaccggcg cgtacgccgg gctcaagccg
     1381 atggacgaga tgatccgccg acccggcgtg ttcggcccca agatcgaacc cccagcgggc
     1441 gccgacgagc agaccgaatt cctctgcttc ctcggccgcc gcgtctgaaa gtcgcgctcg
     1501 gtccgaggtg gaccaggtga ttcggtagct catcggcttg gacgagccgt cgcgggagga
     1561 acagtccggc ccgagggcgc tcgttgaccc tgaatgtgac gttgcctcgc tgtactccgc
     1621 agcggtcctc agacagccgg aagagcctcg ccacgtggcg aggctcctcc gatggccgat
     1681 gtgtgtgggt gggcgtcagc gatctgcggc ctcggtcgcc cgagcgatcg ggctctcggg
     1741 cctgaatccc ttccaggcgc agtagatgcc gagggagagc tcccatgcgg cttcgggcag
     1801 tgtcagcagt cctgacggtc cttcgccgtt cttgtatgcg ccgcacagga tgagcacgtg
     1861 cgacaggatg aggacggggc cgccgatgag gccgatcatt gccatgcgct tcgggacgag
     1921 ttcggatatc acgagcgccg gcccgccgat caggcctagg atcgacatcg cccgcggcac
     1981 gaggcggctc cggtacatca ggtagccgag gatcaagccg ttgccgattc ctgcgacgag
     2041 tccgggtccc cattggaacg cccattcgta catgctggcg tgggagttgc cggcaacggt
     2101 cagggcggtg gcatcgcctc ctgcggacag cgcgttcgcg acgttgacga ccgagatgat
     2161 gctgatgatg ccgatggcgg cgaagacgga ttccatggtg cgagcggcga tgtagctgag
     2221 cgaaagggtt tcgctttgtc gtttgacgag cgaatggatg acgagtgcgg ttccgagttg
     2281 tgtgacgatc aggccgaact cgagaagggc gccgagctta aggctcgcgc tggatnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngaagatg cgtccggttc
     2461 gccgaatatc gacggcggga cccgcggtgt cgctcgtgtc ggcgtttgct gccgctcggc
     2521 ggcccgggtg gggcgtcgcg gtcatcgggg cgcgggctgg cgtgtcggtg attgtggtgt
     2581 tcatgtcagt ggttctcctg atgcgggtga gcgaggctcg gttggtgtgt tgctgggcag
     2641 ttcggtcagt gagatcaggg tgaggccgac gtcgcggagc ttttggagga gtccgtggag
     2701 tgctgactga tcgacgatcg ggnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnng ggctcgaggc ggcctttgac ccggatctcg
     2821 tagtgcggga tcaggtcgtg agcggtcgac gacgaggctg tcgtgttgtc ggtgctgtgg
     2881 tcgtctgcgg tcacatgagc aaggtacgaa cggaccgtgt gaggccgcct cacatcatgt
     2941 ggtgattggc gatgtgattg tgctcgacgc cagttcagag gccgtgttcg gaggcgcggc
     3001 gggttgcttc acgccggctg gtggcaccga gtttggtgta gacgttcttg gtgtgcgtgc
     3061 gcagcgtgtt catcgacacg tgcaactcgc gagcgatctc cgggccgctg agctcgctgc
     3121 gcaacaggcg caacacgtcg agttcgcgac tgctgagttc gtcgacgaat gcaccccggc
     3181 cgacgcgtcg cgtgtctttg gctggctcgc tcggtgggga tgcggcggtg tcctcgatgg
     3241 cggccagcac ccgccgggca taggcggtgg cgtcatcgcc tgacgacacc gatcgcagca
     3301 gtgaggtgac ggacagaccg gcgtgcagga acagacggat gtggccctca agttctgctc
     3361 gcaccagtgc gtcggcgagc gcttcggtgg cggcgctggt gtccccgtgc gcgtcgtacg
     3421 ccgtcgctcg cagaatgagg acctcgatgg cggtcccggt tcgcccgccg ttctcggctg
     3481 caacgagcag ccgatcgagc atgccgatgg cagcgtcgag cgcgctcgtg ctgggggtct
     3541 tgccgtgttg tgcgatgagg atctgggtga gcgtgatgtg ctcgtactcg tgcacgtagc
     3601 tgagttcgtc gtctgtggtc agtccacgtt cggagaccca cgcgagtgcc gaacggaggt
     3661 ctccttgggc gagttggaca cgtgctcgga tcgcagcgac cggtcggacc ggtggcgaga
     3721 agtcggtgtc gtacaggggt gcggcttggt cgatcagttc gagtgccccg tcgatgtcac
     3781 cgtaggcgcg gaggagtcgg gccatggtga cccgccaccg ataggcgtgc tgcgggagcc
     3841 ctgcggactc gcccagctcg ttgctcgtct ccaggtggct ggcggctgcg tcgaggtcgt
     3901 tccgctcgat cagcacttcg ctcaacccgg cgtgcatgtc agcggcgccg cgtaggccgg
     3961 gatgttccgt tgtccagcgg agcccggact caaaggtccg cgtggcgtcg ctgagcttgc
     4021 cctgcgcgat ctggatgtct gccaacgcga gcgagcatcc gagcatgtcc gggacgtgat
     4081 cggcggtaac gagcgcgctg atcgcctcgg tgtagtagcg gacggccgtg tcgagatcac
     4141 cgccggccca gcgggcgagc gctatgagtg cggtggcgct ccctcggcgg aaatggtcgg
     4201 ccggttcgac cagcgcgagg gcttgcgcgg caaacctgct ggtgccgtcg aggtcgccac
     4261 cgagcagggc gaggccggcg cgctggatga ggagttgcgc cgcgaggtgc tcgaacaact
     4321 ccgcgtcgaa cacgatgggc gacggatccg tgcggtgcag cgacgcctcg accgactgga
     4381 gcagtgcctc gacgccggtc gtgtcaccgg tggccattcg cgctccgaca agacacatgg
     4441 caagcaccgg cctgtcagcg aacagatcgt gaggcaatgc ctcgagccat cgccggagga
     4501 cgccctcttg acggctctga cgcatccttg gcgcggcgag ctcaatcagg cgctgcttcc
     4561 tcgatgtggt gaccggccat ggcgtgttcg atcgccttgg cgggttcacc gtgggccgag
     4621 taccacgctc cagctcgtcg gtggagctcg tcgatcaggt ccgggtcttc gtggatcagt
     4681 cgggcgtgga gcacatcggc gaacaggtgg tggtaccggt accagtggcg tcggtcgtcg
     4741 agggcgacga ggaacaggtt ggaacgatcg agctgttcca gcgttgcgcg gccgtcggtt
     4801 ccgcgggtga cggcgtcgca gagtcccccg gtcagccggt cgagcactga ggtgtgcaac
     4861 aggaaggatc ggaccgtgtc gggttggcgg tcgagcactt cttcgacgag gtagtcgacg
     4921 acgaagcggt cgtcgccagc gaagttgtcg atgaacgcgc cgacgtcgtc acgatcgcgc
     4981 agggacagcg cagcgagctg cagcgcggcg atccaccctt cggtcctcgc ttccagcgcc
     5041 ccgacctcga cggcggacag ctgcaggtgc attgaatcgg tgaagtagct cgctgcttct
     5101 tcagtggtga agcggaggtc ggcggcgcgg aactcgagca ggtcgcggcg ggcacgccat
     5161 cgggcgagcg gcagcggcgg atcggcccgg gtcgccagca cgaggtgaaa tcgtggcggt
     5221 gcgtgttcca ccaggaagca gatcgcgtcg tggatgtcgg cagagtcgat gacgtggtag
     5281 tcgtccagca cgagggcgac atcgacgtcg agccattcaa ggtcgttgat gagcgacgcc
     5341 gtgaccgctt ggagtggctg ggagccgtgc aggagcgact cggctgtggc gccgacgtcc
     5401 gaggcggccg atcggagtgc tgccacgacg taggcgccga agacggcagg ttcgttgtct
     5461 gccgcgtcga gcgccaacca ggccgagcga cgatcagcgt ctgccgcgtt cccgaaccac
     5521 tcggtcagca gtgtcgtctt gccgaacccg gccggtgccg agatcagggt cagcgctggt
     5581 tgatgctcgg ccatcatccg gtcagtgagc cggggccggc gaacgatctc gcgtcgtcgg
     5641 cgaggggcgt gcagcttggt tgctaacagc ggaggtcgag cagcaggttc tgctgttgtc
     5701 atcgctcacc gttctgacac ggcgcgacgg cgcaggaagc ggaaccgtga tgatctcgcg
     5761 acgtcggcac ggacagagtc tatgaatccg ccgaaacgct cacggacctg catcgtcgta
     5821 tgggcggatc agtctgggag cgggtgtggt cagttcgttc gaggaccgac gcggcgggcg
     5881 cgcagcacga tgtcgtcgac gtgatgtgtg gaggctgcac cgggcgggcg gggccgcttc
     5941 tcgttgatct cgatctccca ctcggacgaa ctcgcgatcg cttcggcgat gtgatccacc
     6001 cggacgtagg cgtcgggatc gaagcgttgg ctgtgggtgc tgatgtcgat cgggtgtcgc
     6061 atcggttcaa ggtcgtggcc gacgacgagg agtgtcccac cgatggcgac ggcggcgagg
     6121 agattggtga tgcctcggtc gtcgggcgtg cgtgggatcg ccgcgtagtg ggctgagacg
     6181 agatcgaacg atgctgcggg gtacgcgtgc agaccgttgg cgtccgcgtg gtggtatccg
     6241 atctgcaggc cgcgttccgt cgcgatggcg gcgatgcgag ccaacgctcg ctgggagatg
     6301 tcgctggcgg tgacgtccca tccgtgttcg gcgagccaga cggcgtcgcc gccttcgcca
     6361 gcgccgacgt cgagggcacg tcctggcaca gctgcctcga cctctttgac caaggtgccg
     6421 ttcgggttgc cgctccagat ctgtccgccg ccgtagcggt gctcccaatc ggcttggttg
     6481 cccgagggcc gggcggcatt ggccatgtcc tcggcgacca ggctgaagct gatcatcccc
     6541 ccgactcggc tgccgttcgc ggcagcctgc agcacctgtt ggctcgggtc ggtcaggttg
     6601 cctgctgcgt acaagcctgg aacggaggtc tcaccggtgg cgtcggtttc gatgaagtct
     6661 ccgagcccgg tgggatgcgg caccggagtc agacccagcg aagcgaaggg ttcggcgcgg
     6721 acccggaatg gggcgccgac cacgaccgcg tcagcgtcga cctgatcgcc gttggtcagc
     6781 tcgacggcgc agacgtgccc gtcgccgccg gtgacgagcc gccgcaccgg cgtgtcggtg
     6841 acggtcacac cggcggcgcg cagtggggcc agccccggat cgtcggggct gactccggcg
     6901 tgcagcacca gcgtgagttg tgcggtgagt tggcggaaca gcccagcggg atggaggccc
     6961 atcgggtggg taacgatctg gatgatgcgc ttgtcgcgca cctcgtagcc gtgacagaac
     7021 gggcagtgga tgacgtcgcc tccccagtgc tcgccgacac cttcgacatc gggcagaatg
     7081 tcgatcaatc cagtcgcagc aaggacgcgg cgggcgacca cgg
//
LOCUS       DAORNF010000065         7061 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_7891_length_7061_cov_1.426492, whole genome shotgun sequence.
ACCESSION   DAORNF010000065 DAORNF010000000
VERSION     DAORNF010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 7061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7061
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7891_length_7061_cov_1.426492"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>808
                     /locus_tag="PLV68_03895"
     CDS             <1..>808
                     /locus_tag="PLV68_03895"
                     /inference="COORDINATES: protein motif:HMM:NF012372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HRE00415.1"
                     /translation="TRGIKAAGVTTIMAANHNRYKVEVHEANHPDCEHWIADLVDEES
                     GDYHSAAELPKANMLVAGISCVHHSQANSQKAYAEGHNLFTYDDPEWEARVTRSERDR
                     ATAICVLQYADRHRPDVMLIECTTELTSWGPAIQGRPKVGDGSSYRWWLNEVHKLGYK
                     SKTLYLNSQFFGVPQSRDRIYICFWQSTLRTPDLDHRPLAWCHHCDSATESVWSWRTG
                     IPPTGSVRYGKQYDYRCPRCASEVVPPMTPSLAALDLTDLGTRIGDRDKPL"
     assembly_gap    809..900
                     /estimated_length=92
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1026..1775
                     /locus_tag="PLV68_03900"
     CDS             1026..1775
                     /locus_tag="PLV68_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00416.1"
                     /translation="MPTLVAGAGSGGMGILSTGALMMAAGNTYEHPGSECRTRSLDAQ
                     LWTQPATNQVGLLTPPVAVAVDNYQGAPRPVDTPLPTQVGSETMAVVSAGVLPFRKHT
                     IPPTHAEPMPTFTADQVPGLITAAGYIKNNGSIDEAGYRAHPLSSPLGTVVGSATTQG
                     LLFSGWSQQAEIAEWRDRLEQVPLDDCCFRMLFSHEVGYGCGVQVDHPMYGPGDFIVW
                     GSARDQVDGFGNMVSPQVGEWIGARMKAVIA"
     gene            complement(1784..1990)
                     /locus_tag="PLV68_03905"
     CDS             complement(1784..1990)
                     /locus_tag="PLV68_03905"
                     /inference="COORDINATES: protein motif:HMM:NF019057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HRE00417.1"
                     /translation="MASGAKLLVYQRADKKWAWQLKAANGNVIATDGSQGYENVADCR
                     NMATKIVLDGVYHDAEIVDGRVVE"
     assembly_gap    2013..2029
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2030..>2388)
                     /locus_tag="PLV68_03910"
     CDS             complement(<2030..>2388)
                     /locus_tag="PLV68_03910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00418.1"
                     /translation="ESAGALVAAGACVLSAGAADAAVPVAAGAAAVVGFVADVEEQPA
                     LGLHDAADFVEDVGEVGDESGEGVVSADFAVVVEAVLSLFEIGRRGDDGVDAVVGEWD
                     VAGVSVDDHVKRRLPNL"
     assembly_gap    2389..2647
                     /estimated_length=259
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2648..2884
                     /locus_tag="PLV68_03915"
     CDS             <2648..2884
                     /locus_tag="PLV68_03915"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HRE00419.1"
                     /translation="GGDGDPHLAPFPDELARRCILPTTLPGDVVFDPFSGSGTIVRVA
                     ESLGRIGIGTDIYAGRPDLIHGNPVTPPYEAAPA"
     assembly_gap    2891..3039
                     /estimated_length=149
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3188..>3494
                     /locus_tag="PLV68_03920"
     CDS             3188..>3494
                     /locus_tag="PLV68_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00420.1"
                     /translation="MNTTDMPTLDTDVEVIELANLRHQQEQHLRFMARSRHIEKFETK
                     PFPLDRGDAPTIERRLRGIAKALLADNSDAVKWIETGAANMTEKDIVEIITAAFDGVH
                     "
     assembly_gap    3495..3504
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3769..3918
                     /locus_tag="PLV68_03925"
     CDS             3769..3918
                     /locus_tag="PLV68_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00421.1"
                     /translation="MASFVVAERGETPGGCDEETATWWRTAFAEHRRRSRGDTVAVGA
                     GGRQI"
     gene            complement(3924..4490)
                     /locus_tag="PLV68_03930"
     CDS             complement(3924..4490)
                     /locus_tag="PLV68_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00422.1"
                     /translation="MIGSEQIRDDYLRQGDICRLDAFPQWSLEDRTVMAAGKTVSVQM
                     NVADKMASHDGGTLVAVCTQCCDLRVPGKTTGLLLAPVRVPPMSPNDTEMLDELERSN
                     ISRDSRWSFVSLYPLRLGDESQLYVVDFGKMMSLHSPKSSTPALVSSRVYRLTPEARE
                     RFRTKLAASLGRPPTEDESEAIDAVATQ"
     gene            complement(4510..4926)
                     /locus_tag="PLV68_03935"
     CDS             complement(4510..4926)
                     /locus_tag="PLV68_03935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00423.1"
                     /translation="MSLDTLSIRQPAVLRQSRDAGLVELAKSVLRDFDALPRRAQTFV
                     YNTLVGERELGSHPISAAEWLSPRTDTATPALDSSSTIGRLHAIHQFLEAHVVVEDDW
                     DMALMAVPAHVPAEWLAELETWSGDDAATFEIDWDS"
     gene            complement(4923..5564)
                     /locus_tag="PLV68_03940"
     CDS             complement(4923..5564)
                     /locus_tag="PLV68_03940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00424.1"
                     /translation="MIEDISHNIVLVGESFASQKLTVEDFDFGTKSNEVLRLGPMLQV
                     DASPFVALVVPDRIQLGLNGPNITQESSERLVTVAHSLMDVIGRRSVNAVGLNYTGAI
                     RESADSPSALMARLANVSALSSGLNVEVEPPMELILHLRGLSDASTTRLKVWTAAAEG
                     PLLIEVNSHYDLGTNIKTPQVRNALTRFPEAIALSTETALSIERLLAGEAVQR"
     gene            complement(5680..5871)
                     /locus_tag="PLV68_03945"
     CDS             complement(5680..5871)
                     /locus_tag="PLV68_03945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00425.1"
                     /translation="MTPALRSKLRAAAVRLDKAVAERDRLMAEAKAAGASNREIAALV
                     GLSHVGVANILERRANETA"
     gene            complement(5868..6107)
                     /locus_tag="PLV68_03950"
     CDS             complement(5868..6107)
                     /locus_tag="PLV68_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00426.1"
                     /translation="MASSTGQSKQRTAQRGSTTQRRRSRPVDDVGYRETTVSVPNGHG
                     WQRDPVIASAWAHGQQIGWMTKSGDLAPCEATVMA"
     assembly_gap    6235..6353
                     /estimated_length=119
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6354..6633)
                     /locus_tag="PLV68_03955"
     CDS             complement(<6354..6633)
                     /locus_tag="PLV68_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00427.1"
                     /translation="MSASYPRSTRRTALAPAILAVIALLVGVALLTGDLFLIIRFVVS
                     ILALIVAVFAWQARQWWWLIGLIPIAVLWNPVFPIDLGLPDLWLGLQYV"
     assembly_gap    6675..6749
                     /estimated_length=75
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6756..>7061
                     /locus_tag="PLV68_03960"
     CDS             6756..>7061
                     /locus_tag="PLV68_03960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00428.1"
                     /translation="MDQELLATGVPARGEVLGISMSGTTITIMNGPVERNCTFRLRVM
                     RDGAQPYEATVAQRVQEIMISALQQPGVALALKVDPADPQRIAIDFGSAPPEVTLPES
                     "
BASE COUNT         1131 a         2156 c         2003 g         1050 t
ORIGIN      
        1 tcacccgtgg catcaaggcc gcaggcgtca ccacgatcat ggccgccaac cacaaccggt
       61 acaaggtcga ggtccacgaa gcgaaccacc ccgactgtga gcactggatc gctgacctcg
      121 tcgacgagga gtcgggcgac taccactcgg ccgccgaact cccgaaggcc aacatgctgg
      181 tggccgggat ctcgtgcgtg caccactcgc aggccaacag ccaaaaggcc tacgccgagg
      241 gtcacaacct gttcacctac gacgatccgg agtgggaagc ccgggtcacc cgcagcgaac
      301 gtgaccgtgc gacggcgatc tgcgtcctgc agtacgccga ccggcaccgc ccagacgtga
      361 tgttgatcga gtgcacgacc gagctcacgt cctggggtcc ggcgatccag ggccgcccga
      421 aggtcggtga cggctcgagc taccggtggt ggctgaacga ggtccacaag ctcggctaca
      481 agagcaagac cctctatctc aactcacagt tcttcggggt gccgcagtcc cgcgaccgga
      541 tctacatctg cttctggcag tcgacgctgc gcacacctga tctcgatcac cgaccgctcg
      601 cctggtgtca ccactgcgac tcggcgacag agtcggtgtg gtcgtggcgg acaggtatcc
      661 cgccgaccgg ctcggtgcgt tacggcaagc agtacgacta ccggtgcccc cgatgcgcat
      721 ccgaggtcgt tcctccgatg acgccgtcgc tcgccgcgct cgacctcacc gacctcggca
      781 cccggatcgg ggaccgcgac aagccgctnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      901 cagcgaagta gaattccaac ggaatcaacg tggccagcca gcaagaggac gggctgttgt
      961 cggccgggac cgtcgcgatg atgccgaacc gcacgaacgg caccccgtgg cccactgacc
     1021 aaccgatgcc gaccctcgtc gcgggcgcag gctccggcgg gatgggcatc ctgtcgaccg
     1081 gcgcgctgat gatggccgcc gggaacacct acgagcatcc cgggtcggag tgccgcaccc
     1141 gttccctcga cgcccagctg tggactcagc cagccacgaa ccaggtggga ctgctcacgc
     1201 ctccggtcgc ggtcgctgtc gacaactacc aaggggcccc tcgacctgtg gacactccgc
     1261 tgccaaccca ggtggggtcc gagacgatgg cggtcgtgtc cgctggggtg ctgccgttcc
     1321 ggaagcacac gatcccgccg acgcacgccg aaccgatgcc aacgttcacc gccgaccagg
     1381 tcccgggcct catcaccgct gccgggtaca tcaagaacaa cgggtcgatc gacgaggccg
     1441 gctaccgggc gcatccgctg tcgtcaccgc tcggcaccgt cgtcggctcg gccacgacgc
     1501 agggtctgct gttctccggc tggtctcagc aggccgagat cgccgagtgg cgtgatcggc
     1561 tcgagcaggt gccgctcgac gactgctgct tccggatgtt gttcagccac gaggtcggct
     1621 acggctgtgg tgtccaggtg gatcacccga tgtacgggcc gggcgacttc atcgtgtggg
     1681 gttcggcacg cgatcaggtc gacgggttcg gcaacatggt gtcgccacag gttggggagt
     1741 ggatcggcgc ccggatgaag gcggtgatcg cgtgaagtcc ggcctactcc accactctgc
     1801 cgtcgacgat ctccgcgtcg tgataaaccc cgtcgaggac gatcttcgtc gccatgtttc
     1861 tgcagtcggc cacgttctcg taaccctgcg agccatctgt ggcgatcacg tttccgttgg
     1921 cagccttcaa ctgccatgcc cacttcttgt cggctcgttg atacacgagc aacttcgccc
     1981 cactggccat ttggacctcc ccttcggtct acnnnnnnnn nnnnnnnnnc aagttcggga
     2041 ggcgtcgctt cacatgatca tccacgctga cgcccgccac atcccactca ccgaccactg
     2101 cgtcgacacc gtcgtcacct cgccgcccta tctcaaacag cgaaagtacg gcctcgacga
     2161 cgaccgcgaa gtcggccgag acgacaccct cgccggattc gtcaccgacc tcgccgacat
     2221 cttcgacgaa gtccgccgcg tcatgaagcc ccaaggctgg ctgttcctca acatcggcga
     2281 caaagccaac aacagcggcg gcgccggcgg cgactggaac cgccgcgtcg gcggcgccgg
     2341 ccgacagaac gcacgcgcca gcggcgacca aggccccggc cgattcttnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnggc ggcgacggag acccgcatct cgccccgttc ccggacgagc tcgcacgccg
     2701 ctgcatcctc cccacgacgt tgcccggtga cgtcgtgttc gacccgttct ccgggtcagg
     2761 gaccatcgta cgggtagcgg agtcgctcgg ccggatcggg atcgggaccg acatctacgc
     2821 gggtcggcca gatctcatcc acggcaaccc tgtgacaccc ccgtacgagg ctgcaccagc
     2881 atgactgccg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna aaggcgaccc gctcggtgcg
     3061 ccgcgggttc aagctccgcc cgctctgatc cggcgctcgg caactctgta gcacccctct
     3121 ggcagtgttc gtcaacaccc cgcgagcatc atgttcgcac ccaaccgacc gaggaggtca
     3181 cccctcaatg aacaccaccg acatgcccac gctcgacacc gacgtcgaag tgatcgaact
     3241 ggccaacctc cgccaccagc aggagcagca tctccggttc atggcccgca gccgtcacat
     3301 cgagaagttc gagaccaagc cgtttccact cgaccgtggt gacgcaccca cgatcgaacg
     3361 tcgcctccgc ggcatagcca aggcgctgct cgccgacaac tccgacgccg tgaagtggat
     3421 cgagaccggg gccgcgaaca tgaccgagaa ggacatcgtc gagatcatca ccgcagcgtt
     3481 cgacggtgtc catgnnnnnn nnnncgatcg aggggatctg ctgactgtcg tcccgccccc
     3541 gtcgaacccc gttccacccc acaagatcgt cgacgtcgtc aaccgctact accaactgct
     3601 tcggctcgcc gaaaacgttc gcttctatcg gttcaagtcg gagggccgtg tcgggacggt
     3661 tctctgacag gtgggggcag gaccggaccc gctcccgccc gtcgacaggt ccgcgctgac
     3721 gttggcgacc tcgtcgctgg gctccgtgcg cttgagcgtc ggttcttgat ggcgtcgttc
     3781 gtggtggccg agcgcggcga gacgccgggc ggctgcgatg aggagacggc gacgtggtgg
     3841 cggacagcgt tcgccgagca ccgccgccgc agccggggag acaccgttgc tgtcggcgcc
     3901 ggcggtcggc agatttgaat cccctattgc gtcgccaccg cgtcgatcgc ttcgctctca
     3961 tcctccgtcg gcgggcgacc gagtgatgcc gcaagcttcg tgcggaagcg ttcgcgcgct
     4021 tcgggcgtga ggcgatacac acgcgaagaa accagagccg gcgtcgatga cttggggctg
     4081 tgcagcgaca tcatcttgcc gaagtccacc acgtacaact gagattcatc gcccagccgc
     4141 aacgggtaga ggctcacaaa gctccaacgt gagtcacgac tgatgttcga ccgctccagt
     4201 tcgtcgagca tctcggtatc gttcggagac atcggcggaa cacggaccgg cgcgaggagc
     4261 aggccagtgg tcttgccggg cacccgaagg tcgcagcatt gggtgcagac cgcaaccaac
     4321 gtcccgccat catgcgacgc catcttgtcc gccacgttca tctggacgct caccgtcttg
     4381 cccgccgcca tgacggtgcg gtcttcgagc gaccattgcg ggaacgcgtc aaggcggcag
     4441 atgtcgccct gtcgtaggta gtcgtctcgg atctgctccg acccaatcac ctcgcccctg
     4501 gtgaaagagt caggagtccc agtcaatttc aaacgtcgct gcgtcgtcgc cggaccacgt
     4561 ctcgagctcc gccagccatt cagctggaac gtgagcagga acagccatga gtgccatgtc
     4621 ccagtcgtct tcgacgacga cgtgcgcctc gagaaactga tgaatggcgt gcagccggcc
     4681 gatcgtgctc gatgagtcga gagccggggt ggctgtgtcc gttcgcggcg agagccactc
     4741 agctgcagag atgggatgcg agccgagttc gcgctcgcct accagcgtgt tgtagacgaa
     4801 cgtctgcgcc cgacgcggca gtgcgtcaaa gtcccgcaga accgatttgg cgagctcgac
     4861 caatcccgca tcgcggctct gtcgaagaac tgcgggctgc ctaatggaga gcgtgtcgag
     4921 actcatcgct gaaccgcctc gccagcgaga agacgttcga tcgacaacgc ggtctctgtt
     4981 gagagagcga tcgcctcagg gaaccgagta agtgcatttc ggacctgagg tgtcttgatg
     5041 ttcgtcccca agtcgtagtg actgttgact tcgatgagga gaggaccttc ggcagccgct
     5101 gtccagacct tcaggcgggt ggtcgaggcg tcgctcaggc ccctgaggtg caggattagc
     5161 tccatgggtg gctcgacctc gacgttcagc ccgctcgaca acgccgagac gttggcaagc
     5221 cgcgccatca gcgcgctggg tgagtcagcc gactcacgta tcgcgcccgt gtagttcagg
     5281 cccacggcat tcaccgagcg tcggccgatg acgtccatca ggctgtgcgc aacggtgacc
     5341 agcctctcac tgctctcctg cgtgatgttc gggccgttca agcccaattg gatacggtca
     5401 ggcaccacga gcgctacgaa tggggaggcg tcgacttgca acattggtcc gaggcgcaga
     5461 acctcattgc tcttagtgcc gaaatcgaag tcttccacag tcagtttctg gctcgcgaac
     5521 gactcaccga ccaagacgat gttgtgactg atgtcttcga tcactgtaac tggctcctac
     5581 gtgctggccc gagagcacgc cgacatccgt gaagatagcg cgttgacacc cacgcgcggt
     5641 catcggcatg cggtcgcccg cagttgagcg aatcgttggt caggcggtct cgttcgcgcg
     5701 gcgttcgagg atgttcgcga caccgacgtg cgacagaccg accagcgccg cgatctcccg
     5761 gttcgacgcg ccagcggcct tcgcctcagc catcagccgg tcccgctcgg cgacagcctt
     5821 gtcgagccgc accgccgcgg cgcggagctt gctacgcagg gccggagtca tgccatcacc
     5881 gtagcttcgc acggggcgag gtcgcccgac ttcgtcatcc agccgatctg ctggccgtgg
     5941 gcccatgcag aggcgatgac ggggtcgcgc tgccagccgt gaccgttggg gaccgagacg
     6001 gtcgtctcgc ggtagccgac gtcgtcgacg ggccgtgagc ggcggcgctg ggtcgtcgaa
     6061 ccgcgctgcg cggtgcgctg cttggactgg ccggtggatg atgccacgtg ttctccccgc
     6121 gaaccgtgac ggcccgcgtt cccttagtga acagtctgcg gtgcctcacc gcgagacggc
     6181 gacgggagcg catccagacc cggccgcggt ttgcagccgg tggaggtcac cgtcnnnnnn
     6241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnccacgta
     6361 ctgcaatccg agccacagat ccggcaggcc gagatcgatc gggaacaccg ggttccacag
     6421 caccgcgatg gggatgagac cgatgagcca ccaccactgg cgggcctgcc acgcgaagac
     6481 cgcgacgatg agcgcgagga tgctcaccac gaaccggatg atcagaaaga ggtcgcccgt
     6541 cagcagcgcg acgccgacga gaagggcgat gacggcgagg atcgcggggg ccagcgccgt
     6601 ccgccgggtg gatcggggat acgacgcgct catgacttca ccctagggcg tcgcgccggg
     6661 acgaccccgc gcgcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnng ggggcgtgga tcaggagctg ctggccacgg
     6781 gcgtccccgc gcgcggcgag gtgctcggga tctcgatgtc gggcacgacg atcacgatca
     6841 tgaacgggcc cgtcgaacgc aactgcacgt tccgtctgcg ggtcatgcgc gatggcgccc
     6901 agccctacga ggccaccgtc gcccagcgag tgcaggagat catgatctcg gcgttgcagc
     6961 agccgggtgt ggcgctcgcg ctcaaggtgg atcccgccga ccctcagcgg atcgccatcg
     7021 atttcggctc cgcgccgccg gaggtgaccc tgcccgagtc g
//
LOCUS       DAORNF010000066         7012 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_7978_length_7012_cov_1.774041, whole genome shotgun sequence.
ACCESSION   DAORNF010000066 DAORNF010000000
VERSION     DAORNF010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 7012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7012
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7978_length_7012_cov_1.774041"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..943)
                     /locus_tag="PLV68_03965"
     CDS             complement(<1..943)
                     /locus_tag="PLV68_03965"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HRE00429.1"
                     /translation="MDSPAEQIVVRRSGPLRGDVTVPGAKNSVLKLMATTLLADGTTT
                     LTNVPDIADVAIMADLLAAIGVVTRWDDTRSRVLHLTNAGQITPVAPYELVERIRASI
                     NVLGPLLTRCGTVRLSLPGGDDFGSRPINMHLDGLTRMGASFEICHGYVEGHAERLHG
                     ADVVFDFPSVGATENILTAAVFAKGTTTIDNAAREPEIADLCHFLVAMGADIEGIGSS
                     KLVIHGVEPGSLVPVSHEVVPDRIQAATYVAAAAITGGQIDVHNARPEHMQTLLRRFT
                     DMGLVIEPLVGGLRVIAPEQLRSIDVATLPYPGIATDY"
     gene            965..>1546
                     /locus_tag="PLV68_03970"
     CDS             965..>1546
                     /locus_tag="PLV68_03970"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRE00430.1"
                     /translation="MPRTKRIERQVLPAEHTTALTPRDDLVLEDEAVSTAAGGVTTTR
                     FGLAEGPLTHYARTVVDDGTTVHETTEYRLAIPWFRWLFAWPVSRALKARHDPGVHPG
                     SQPWWAPPDRLEPRQAMLLAKLRNGAAAADARMALEVATIGGAGCLGRIGEIGSLAVG
                     AVGDVAIWSLTGPAFAGAIADPIEAWLRCGPLVA"
     assembly_gap    1547..1646
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1909..2619
                     /locus_tag="PLV68_03975"
     CDS             1909..2619
                     /locus_tag="PLV68_03975"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HRE00431.1"
                     /translation="MLISRRTMLLATLAATAAACTTRTQLGKSGSPVELVGPGPYSSS
                     DLEELSQLAGEVEGIPNSVMVVGDSISAGSRQVLDTVLTDMGFDEVHIDAEPSRRIQV
                     GKSKPTNGLAIVEFVQGAQPPGIWVVELGTNDAGLYAEQDEYAELIDSVLDVIGDRVD
                     LVWVNIYRFDVVDACRSFNTALVERLSARGNATIVDWFSQCLDHAGDLLSDGVHPNAA
                     GQLVLADMIRQAIQTRVR"
     gene            complement(2629..3321)
                     /locus_tag="PLV68_03980"
     CDS             complement(2629..3321)
                     /locus_tag="PLV68_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004105653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRE00432.1"
                     /translation="MSAEPGDMLAVFPETPPPDLARTLDLAGYRWQGIAGPDQATRDE
                     PAGGWAGAVVCADANPDAAWALSRALRKRDQPVQPLLLLVGGGQLAELALRDDLFDDF
                     CLTPFHPVELEARLRHLLFKRGGGSRPEVVEHSGLALNVETYQASIMGRPLDLTYMEY
                     ELLKFLAQNPGKVFTRELLLSRVWGYEYYGGARTVDVHIRRLRAKLGEEHQALIQTVR
                     SVGYRFGQSRWD"
     gene            complement(3318..4655)
                     /locus_tag="PLV68_03985"
     CDS             complement(3318..4655)
                     /locus_tag="PLV68_03985"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HRE00433.1"
                     /translation="MNEQQRNYVLHTVEERGVRLIRLWFTDVLGNLKSFAISPAELEN
                     ALEDGMTFDGSSIDGFSRVQESDVLAIPDATTFEVLPWGDPKQAEARVFCDIHNLDGS
                     PFQGDPRQVLRRNLTEARERGLTCFVAPDMEYFYFAPPQPGCPPEPLDHASFFDLTTS
                     DVSGTLRKQTIRTLELMSIPVEYSFHEDAPSQHEIDLRHNDALSMADAVMTFRMVVRE
                     VAASQGVHASFMPKPLQGVQGSGMHMHLSLFDGDTNAFYDADDPYNLSLIAKQFMAGL
                     LRHAAEITAVTNQTVNSYKRLVPGFEAPVHVSWARNNRSGLIRVPIPKKGNPNATRIE
                     YRSPDPACNPYLAFSVVLAAGMRGIREGYELPAEAGANLFQLTDAELLALGIDQIPAS
                     LGDALKVMERSDLVAEALGSHIFEWFLRNKRNEWQDYKAHVSTFETDRYLRSL"
     gene            complement(4740..5747)
                     /gene="glnII"
                     /locus_tag="PLV68_03990"
     CDS             complement(4740..5747)
                     /gene="glnII"
                     /locus_tag="PLV68_03990"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase GlnII"
                     /protein_id="HRE00434.1"
                     /translation="MAYQAEYIWLDGTTPTALLRSKTKILKDGASPLPIWGFDGSSTN
                     QADGAHSDCVLNPVFSCPDPLRGGDNILVMCEVLSAETMKPHATNTRAPLEKVAKKYA
                     KHEMIFGLEQEYTMLRADGTPLGFPEGGGFPAPQGPYYCGVGTGRVIGREIIEEHTQA
                     CLDAGLLIEGTNAEVMPGQWEFQIGAADGLTVGDHMYVARWLLHRIAEDYDVIITLAA
                     KPQKGDWNGAGMHTNFSTKAMREGYPAIDAACKAIGKKIDVHVNGYGDDIQSRLTGKH
                     ETAPWNQFSYGVSNRGASIRIPWQVERDGKGYAEDRRPNANADPYTVARLLIDTVCSA
                     A"
     gene            6007..>6252
                     /locus_tag="PLV68_03995"
     CDS             6007..>6252
                     /locus_tag="PLV68_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HRE00435.1"
                     /translation="MTRLRIAAAQINPIVGDLAGNEAKILDAYRRADDAGCDIVAFPE
                     LSVTGYPPEDLVLKPGFITDAKDALARIAAATGRCVAV"
     assembly_gap    6253..6262
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6263..>7012
                     /locus_tag="PLV68_04000"
     CDS             <6263..>7012
                     /locus_tag="PLV68_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HRE00436.1"
                     /translation="DSDRDLYNAAAVCAGGRVLGTYRKRLLPNYAVFDEARYFTPGHD
                     SDPLELYVVGGVKVGISICEDIWSPAGPLATQAAGGAELNVNINASPFHAGKVAHRER
                     MLATRAADASCALLYVNQVGGQDELVFDGASMVFDADGELIGRAGQFVEQVLITDIEI
                     EPVYRKRLLDPRGRETDALLPLIVVSETASSPSSPPSAIGATAAMPIAPLLDADAELY
                     EAIVLATGDYIRKNGFTDVVIGLSGGIDSTLV"
BASE COUNT         1059 a         2250 c         2416 g         1177 t
ORIGIN      
        1 tgtagtcggt ggcgatgccg ggatacggca gtgtcgcgac gtcgatcgaa cggagctgct
       61 cgggtgcgat cacgcgcagc ccgccgacga gcggttcgat caccaggccc atgtcggtaa
      121 agcggcggag cagggtttgc atgtgctcgg gccgggcgtt gtggacgtcg atctgcccgc
      181 cggtgatcgc cgccgcggcc acgtaggtcg cagcttggat gcggtcgggg acgacttcgt
      241 ggctgaccgg caccaggctg cccggctcga ccccgtggat caccagcttg gaactgccga
      301 tgccttcgat gtcggcgccc atcgcgacca ggaagtggca caggtcagcg atctccggtt
      361 cacgagccgc gttgtcgatc gtggtcgtgc ccttggcgaa cacggccgcc gtcaagatgt
      421 tctcggtggc cccgacgctg gggaagtcga acaccacgtc ggcgccgtgc agccgctcgg
      481 cgtgaccttc gacgtacccg tggcagatct cgaagctcgc ccccatgcgg gtcagcccgt
      541 cgaggtgcat gttgatcggg cggctgccga agtcgtcgcc gccgggcagc gacaggcgca
      601 ccgtgccaca ccgggtcagc aaggggccga gcacgttgat cgaggcccgg atccgctcga
      661 ccaactcgta cggagcgacc ggtgtgatct gcccggcgtt ggtcaggtgc aggacgcggg
      721 agcgggtgtc gtcccatcgg gtgacgacac cgatcgcggc cagcaggtcg gccatgatcg
      781 ccacgtcggc gatgtcgggc acgttggtga gcgtggtcgt gccgtcggcc agcagcgtgg
      841 tcgccatcag cttgagcacc gagttcttcg cgcccggcac ggtgacgtcg ccgcgcaacg
      901 gcccgctgcg gcgcacgacg atctgctcgg ccggtgaatc catggccgtc agtatgctcc
      961 cgaggtgccc aggaccaagc gcatcgagcg acaagtcctg ccagccgagc acaccaccgc
     1021 gctgacgcca cgagacgact tggtgctcga agacgaggcc gtcagcaccg ccgccggcgg
     1081 ggtcaccacc acccgcttcg gcctggccga aggcccgctc acgcactatg cgcgcaccgt
     1141 cgtggacgac ggcaccaccg tgcacgagac caccgagtac cggctcgcga tcccctggtt
     1201 ccggtggctg ttcgcgtggc cggtgtcacg cgcgctgaag gcccggcacg accccggtgt
     1261 ccatccgggg tcacagccgt ggtgggcgcc gcccgatcgt ctggaaccgc gccaggcgat
     1321 gctgctggcc aagctgcgca acggtgccgc cgcggcggat gcgcggatgg cgctggaggt
     1381 cgccaccatc ggcggtgcgg gctgcctggg ccgcatcggc gagatcgggt cgctggcggt
     1441 gggtgcggtc ggcgacgtcg cgatctggtc gttgaccgga cccgcgttcg cgggggccat
     1501 cgccgacccc atcgaagcct ggctgcgctg cgggccgctc gttgccnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnncgta gccaacaccc tcacctcgca gggtgagcac
     1681 ctggtcgatc accgccgggt cagtgagcgc atccaacgcc tcaccgtctg accaccgctc
     1741 gacagggcgg gtgtgtccga ttggcccctc ctggcggggt caagtgaaca cacctgcgcg
     1801 gtcggtgcga gacggtgttc gattggcctt tccacgcggg gtcaactgaa cacacgtgga
     1861 cgggagggtg acgttgcggc gggtgcgtcg tggcccagac tgtgcggcgt gttgatctct
     1921 cgccgcacga tgctgctggc aacgctcgcg gcgacggctg ccgcctgtac gacccgtact
     1981 caactgggca agtccggctc gccggtcgag ctggtcggtc cgggtcctta ttcgtccagc
     2041 gacctcgaag agttgagcca gctcgcgggt gaggtcgagg gcatccccaa ctcggtgatg
     2101 gtggtcggcg acagcatctc ggccggctct cgtcaggtgc tcgacacggt gctcaccgac
     2161 atgggcttcg acgaggtgca catcgacgcc gagccgagcc gccggatcca agtcggcaag
     2221 tccaagccga ccaacggact ggcgatcgtc gagttcgtgc agggtgccca accgcccggc
     2281 atctgggtag tcgaactcgg caccaacgac gcagggctct acgccgaaca ggacgaatac
     2341 gccgaactga tcgactcggt gctcgacgtg atcggcgacc gagtcgatct ggtgtgggtc
     2401 aacatctatc gattcgacgt ggtggacgcg tgccgctcgt tcaacaccgc gctggtcgag
     2461 cggctcagcg cacgcggcaa cgccacgatc gtcgactggt tcagccagtg cctcgaccat
     2521 gccggtgatc tgctgagcga cggcgtccac cccaacgcgg cgggccaact cgtgctggcc
     2581 gacatgatcc gccaagccat ccaaacccgc gtccgctgac gcgggtggtc agtcccagcg
     2641 ggattggccg aagcggtagc cgacgctgcg gacggtttgg atcagggctt ggtgctcctc
     2701 gcccagcttc gcccgcagtc gtcggatgtg cacgtcgacg gtccgtgcgc cgccgtagta
     2761 ctcgtagccc cacacccggc tgagcagcag ctcgcgggtg aacactttgc cggggttctg
     2821 agccaagaac ttgagcagct cgtactccat gtaggtcagg tcgagcgggc ggcccatgat
     2881 cgaggcctgg taggtctcga cgttgagggc caggccgctg tgctcgacca cctcgggccg
     2941 cgaaccgccg ccgcgcttga acagcaggtg gcgcagccgg gcttcgagct cgaccggatg
     3001 gaacggcgtc aggcagaagt cgtcgaacag gtcgtcgcgc agggcgagct ccgcgagttg
     3061 gccgccaccg accaacaaca gcagcggctg caccggctgg tcgcgcttgc gcagcgcgcg
     3121 cgacagcgcc caggctgcgt cggggttggc atcggcacag accactgcac cggcccagcc
     3181 gccggccggt tcgtcgcgcg tcgcctgatc gggaccggcg atgccctgcc agcgatagcc
     3241 agccaggtcg agagtgcgag ccaagtcggg cgggggcgtc tccggaaaga ccgccagcat
     3301 gtcgcccggc tctgcgctca cagcgacctc aagtagcgat cggtctcgaa ggtgctgacg
     3361 tgggccttgt agtcctgcca ctcgttgcgc ttgttgcgca ggaaccattc gaagatgtga
     3421 ctgccgagcg cttcggccac caggtcggaa cgctccatca ccttcagcgc gtcgccgagc
     3481 gatgcgggga tctggtcgat gcccagcgcg agcaactcgg cgtcggtgag ctggaacagg
     3541 ttggccccag cctcggccgg tagctcgtac ccctcgcgga tgccgcgcat gccggccgcg
     3601 agcaccacgc tgaacgccag gtaggggttg caggccgggt cgggggagcg gtactcgatg
     3661 cgggtcgcgt tcgggttgcc cttcttgggg atcggcaccc ggatcagccc gctgcggttg
     3721 ttgcgtgccc aactgacgtg cacgggcgct tcgaagcccg gcaccaaccg cttgtagctg
     3781 ttgaccgtct ggttggtgac ggcggtgatc tcggccgcgt gacgcaacag cccggccatg
     3841 aactgcttgg cgatcagcga caggttgtac gggtcgtcgg cgtcgtagaa cgcgttggtg
     3901 tcgccgtcga acagcgacag gtgcatgtgc atgccgctgc cctgcacacc ctgcagcggc
     3961 ttgggcatga acgaggcgtg caccccctgg ctcgcggcga cctcgcgcac caccatgcgg
     4021 aacgtcatca ctgcgtcggc catgctcagc gcgtcgttgt gccgcaggtc gatctcgtgt
     4081 tggctcggcg cgtcttcgtg gaagctgtac tcgaccggga tgctcatcag ttccagcgtc
     4141 cggatcgtct gcttgcgcag cgttcccgac acgtcgctgg tggtgagatc gaagaagctg
     4201 gcgtgatcga gcggctccgg agggcagccc ggctgcgggg gagcgaagta gaagtactcc
     4261 atgtcggggg cgacgaagca cgtcagcccg cgctcgcgcg cctcggtcag gttgcgccgc
     4321 agcacctggc gcggatcgcc ctggaacggc gagccgtcga ggttgtggat gtcgcagaac
     4381 acccgcgcct cggcctgctt cgggtcgccc cacggcagca cttcgaaggt cgttgcatcc
     4441 ggaatggcca gcacgtcgct ctcttggacg cggctgaagc cgtcgatcga cgacccgtcg
     4501 aacgtcatgc cgtcttcgag tgcgttctcg agttcggccg ggctgatcgc gaagctcttc
     4561 aggttgccga gcacgtcggt gaaccacagg cgaatcaggc gcacgccacg ctcttccacg
     4621 gtgtgcagca cgtagttgcg ttgttgctcg ttcatcacca ttgcctcttc acgttagagc
     4681 gccgtgggcg cgaagacgcc cgggccgggg gcggtgatcc ccgacccggg cgtggagtgt
     4741 cacgccgcgc tgcagaccgt gtcgatcagc agacgagcga ccgtgtacgg gtcggcgttg
     4801 gcgttggggc ggcggtcttc cgcgtagccc ttgccgtcgc gctcgacctg ccacgggatg
     4861 cggatcgagg cgccacggtt cgacacgccg tagctgaact ggttccacgg ggcggtctcg
     4921 tgcttaccgg tgaggcggct ctggatgtcg tcgccgtagc cgttgacatg cacgtcgatc
     4981 ttcttgccga tcgccttgca ggccgcgtcg atcgccgggt aaccctcgcg catcgccttg
     5041 gtggagaagt tggtgtgcat gccagcgccg ttccagtcgc ccttttgcgg cttggcggcc
     5101 agggtgatga ttacgtcgta gtcctcggcg atgcggtgta gcagccagcg ggcgacgtac
     5161 atgtggtcgc cgaccgtgag cccgtcggcg gcgccgatct ggaactccca ctggccgggc
     5221 atgacctcgg cgttggtgcc ttcgatcagc agtccggcat cgaggcaggc ctgggtgtgc
     5281 tcttcgatga tctcgcgacc gatgacgcgc ccggtgccga cgccgcagta gtacggaccc
     5341 tgcggggcgg ggaagccgcc gccctcgggg aagccgagcg gcgtgccgtc ggcgcggagc
     5401 atggtgtact cctgctcgag gccgaagatc atctcatgct tggcgtactt cttggcaacc
     5461 ttctccaacg gggcgcgggt gttggtcgcg tgcggcttca tggtctcggc ggagagcact
     5521 tcgcacatca ccaagatgtt gtcgccgccg cgcagcggat cggggcagga gaacaccggg
     5581 ttgaggacac agtcgctgtg cgcgccgtcg gcctgattgg tggaggaacc gtcgaagccc
     5641 cagatcggca gcggcgatgc gccgtccttc aggatcttgg tcttcgaacg gagcaaggcg
     5701 gtgggggtcg taccgtccag ccagatgtat tcagcctgat aagccatggg tgggtgtcct
     5761 ttgaggggag ccggttcgat cagcgacgac gtgtcgttga aggggcgacg gcactctgcc
     5821 aagcagcgat ttcgacgccg ttgcctgttt gtgaacgccg tgtgaaccgc ctgcccaggt
     5881 accttctgca aagtcggccg aggcgtcctg gcgcccgcac gagggtgtcg tgagcgcgcc
     5941 ttgaacgtgg cgagagcgtg atggagccag cgggcgtgcc gtagcgggcg gcatagattg
     6001 gcgggcgtga cacgactgcg gatcgctgcg gctcagatca atcccatcgt cggcgacctg
     6061 gcgggcaacg aggccaagat tctcgacgcg taccggcgag ccgacgacgc gggatgcgac
     6121 atcgtcgcgt tccccgaact gtcggtgacg gggtatccgc ccgaggatct ggtgctcaag
     6181 ccgggcttca tcaccgacgc caaggacgcg ctcgctcgca tcgctgccgc caccggacgc
     6241 tgcgtggccg tgnnnnnnnn nncgacagcg accgcgatct gtacaacgcg gcggcggtct
     6301 gcgctggggg tcgggtgctc ggtacctacc gcaagcgact gctgccgaac tacgccgtgt
     6361 tcgacgaggc tcggtacttc acgccgggcc acgacagcga tccgctggag ttgtacgtgg
     6421 tcggtggggt caaggtcggc atcagcatct gcgaggacat ctggagcccg gccggtccgc
     6481 tcgccacgca ggctgcgggc ggagccgagt tgaacgtcaa catcaacgcc tcgccgtttc
     6541 acgcgggcaa ggtcgcccat cgcgaacgca tgctggccac ccgcgccgcc gacgcgagtt
     6601 gtgcgctgtt gtacgtcaac caggtcggcg gccaggacga gttggtgttc gacggagcct
     6661 cgatggtgtt cgacgccgac ggtgagctga tcggccgcgc aggccagttc gtcgagcagg
     6721 tgctgatcac cgacatcgag atcgagccgg tgtaccgcaa gcggttgctc gacccgcgtg
     6781 gacgcgagac cgacgcgctc ctcccgctga tcgtcgtatc cgagacggcg tcatcaccat
     6841 catcgccgcc gtctgcgatc ggcgccacgg ccgcgatgcc gatcgccccg ctgctcgacg
     6901 ccgacgccga gctgtacgag gcgatcgtgc tcgccaccgg cgactacatc cgcaagaacg
     6961 gcttcaccga cgtggtgatc ggcctgtcgg gtggcatcga ctcgacgctg gt
//
LOCUS       DAORNF010000067         6986 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_8023_length_6986_cov_1.680277, whole genome shotgun sequence.
ACCESSION   DAORNF010000067 DAORNF010000000
VERSION     DAORNF010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 6986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6986
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8023_length_6986_cov_1.680277"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..536
                     /locus_tag="PLV68_04005"
     CDS             <1..536
                     /locus_tag="PLV68_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00437.1"
                     /translation="ALPTTSKERGNELTLLKLHGSVDWLRVADQKPKTVDDDYRPLTT
                     LTTKTDNVGKLPTAGEEIIRVSWDLNTAWQRIKARAETPHIVTMATGKIDDLGPLEQV
                     WRDAYSALSMAKELHIVGYSMPTDDTEIRTLLRAGVQRGTKRPTVLVRNPSPDVHERV
                     RRFVLPAIWSDFSSIVR"
     gene            complement(601..2136)
                     /locus_tag="PLV68_04010"
     CDS             complement(601..2136)
                     /locus_tag="PLV68_04010"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HRE00438.1"
                     /translation="MVAKRQSNARTGAGALFGRAAIYARISLDKVGEAAGVSRQIDSC
                     RHLADLEGWSVDEVLVDNDLSAYSGACRPEYERLLDLVRRREIDVIVTYHPDRLYRRL
                     ADLVELTKVVASAGVEIRTVAAGHVDLNTASGRFTAQVLGAAAEHEFARIGERVSAKH
                     HQNAERGNAHGGGRSYGYRRVAQGRIEIVPEEADVLREAAARVLGGQSLASIVVDLNE
                     RRTPSTHGKKWRPGSLGMILKSGRITGLRESKGTVVGEANWEPILDRPTWEQVCSRLA
                     HAPIGRQPKVNLLAGMCRCAECGAVMAAVSERMDKQLKPGQRPPRRTLACDKANRPGC
                     GSNTVKADYVEQIVVDYVLAALEHTDIATARARRAGGDAHQLVASIETDEQFLADLAA
                     DYGAQRINRAQFLAAQDPIHARLLLARRRLDQLTVDPNLDGTAFEGVTADSWDRLDLD
                     QQRALVGLFIEHVVIRKGKRGRYFDPSRVQPVPGENGVSRGNKPQTSPLFATPPLHDS
                     SPD"
     gene            complement(2213..3070)
                     /locus_tag="PLV68_04015"
     CDS             complement(2213..3070)
                     /locus_tag="PLV68_04015"
                     /inference="COORDINATES: protein motif:HMM:NF013328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HRE00439.1"
                     /translation="MPELPEVQAHAERLTEAFAGRVLRRFTPLTFTALKTAVPQPSDA
                     YGLALQRVGRRGKYLLLEFEPVTFVVHLMQGGRLLVDTKQSAKPRGGQARFNFEPPED
                     GSPDAALLLTEQGTERRAGVWCVPTATALTDLPLSKLGPDALDLDTAAMQQRMREFPM
                     RLHGFLRDQHMIAGLGRMLANEVCHRAKLSPFANTGKLGADDVAAVVTAIHACVTDGL
                     AYERTRTDMSSSKDRPGNVHGRIGEPCNVCGDTIRSVAYSSYTVAYCPTCQTNGKVLA
                     DNTTSKFLR"
     gene            complement(3083..3475)
                     /locus_tag="PLV68_04020"
     CDS             complement(3083..3475)
                     /locus_tag="PLV68_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HRE00440.1"
                     /translation="MRYLSLEWIEAVAAEAAVSDAVRDAAEECTVGVTQVVTDGPEGT
                     VIYSLQSSGDGAFTFTAGQAFPEDVRFQQTWETAVGVATGALNAQEAFIQGRILLTGD
                     QQKLMDSQPVFAALSPIFDRVRERTDYT"
     gene            3600..4370
                     /locus_tag="PLV68_04025"
     CDS             3600..4370
                     /locus_tag="PLV68_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00441.1"
                     /translation="MPGEGVSAGRVSAGEISAERVPARGVRAETDGFLVVDEQRHTDA
                     QVEAWWFWGADETGEAGVYAGLELRGRRVDYWAGMVRTGSPYLYVEELDSASLRQGLE
                     IKPPEMWAGHHCDVPFRQWSVGNEAHGVLLDDPTEAWRRAYGLPVPTTFDIEWYGEPD
                     TVAPLAGLAPGARGYRQTGSVDVEIELLEGVLRFSGPGQRVHVWGQAYLPTSFAMPID
                     VHGLRAPYRRSDGRLVDQVLTDRWWVSVRDLSAGEARP"
     gene            4905..5225
                     /locus_tag="PLV68_04030"
     CDS             4905..5225
                     /locus_tag="PLV68_04030"
                     /inference="COORDINATES: protein motif:HMM:NF022740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3097 family protein"
                     /protein_id="HRE00442.1"
                     /translation="MVDHLVAGSKEARIAAAVRDPNVLITGHPFVDVWAGIRPKVMGL
                     TAWPDVPRGQPWKEGMCRALGTDLATFWPTLRNRVTTYADLTPELVGAVERLIDFVAD
                     TPDP"
     gene            complement(5326..>5520)
                     /locus_tag="PLV68_04035"
     CDS             complement(5326..>5520)
                     /locus_tag="PLV68_04035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00443.1"
                     /translation="HGWPASVFDLMLPRMTLGGPDEVGEQLSNLIAHGLDGITLNLVA
                     NAHDPDMIALAAQVSNAALG"
     assembly_gap    5521..5534
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5535..6293)
                     /locus_tag="PLV68_04040"
     CDS             complement(<5535..6293)
                     /locus_tag="PLV68_04040"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HRE00444.1"
                     /translation="MTRLGYQIPNFTYPGVAPDQIFPNLVRQVQAAEASGFDRILLMD
                     HFYQLPGLGDPDDYMLECYTTLAALAQHTKTARLAALVTGNTYRNPAVLAKIVTALDH
                     VSGGRATLGIGAGWFQLEHDSFGIPFDTFSTRFEKLEEALQIILPMLRGERPTLHSTH
                     YHVTEAINSPAPVSKIPVMIGGGGERKTLRMVAQYADESNLTSQPEDIPRKLDVLAEH
                     CANLGRDRSEIVVTKLVMMVIGNTHEEAVAAAHEF"
     assembly_gap    6546..6612
                     /estimated_length=67
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6613..>6986)
                     /locus_tag="PLV68_04045"
     CDS             complement(<6613..>6986)
                     /locus_tag="PLV68_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HRE00445.1"
                     /translation="ICTAASLRELATPALLAVLTPVIIGFGINFYALGAFLAAVILVG
                     QLMANYLSNAGGAWDNSKKYIEDGHHGGKGSDPYRAAVIADTVGDPFKDTAGPALNPL
                     IKVMNLVSLLVLPAIISLQDND"
BASE COUNT         1090 a         2207 c         2417 g         1191 t
ORIGIN      
        1 gtgcgctgcc cacgaccagc aaggagcgag gcaacgaact aacgctattg aaactccatg
       61 gatcggtcga ttggctccgg gttgcagacc agaaaccgaa gacggttgac gatgactaca
      121 ggcccctaac gactctcacc acgaagaccg acaacgtggg caagctccct accgctggcg
      181 aggagatcat ccgcgtctca tgggacctga acacagcctg gcagcgcatc aaagctcgtg
      241 cggagacgcc gcacatcgta acgatggcaa caggcaagat tgacgatctc ggcccgctgg
      301 agcaggtgtg gcgcgacgcg tactcggctc tgagcatggc gaaggagttg cacatcgtgg
      361 gttactcgat gcccactgat gacaccgaga tccgcacact tctgcgcgct ggcgtccagc
      421 gcggcacgaa gcgaccaacc gtgttggtcc gaaatccatc ccccgacgtg cacgagcgtg
      481 tacgccggtt cgtgcttccg gccatctggt cagacttctc gagcatcgtg cgctgagtcg
      541 cccactgacg cgggtctccc atcggcggat gccgctccag tcccagcctc agcgttgcag
      601 tcaatctggg cttgagtcgt gcaacggtgg ggtcgcgaac agtggcgagg tctgcggctt
      661 gttccctcgt gagacgccgt tctcgcccgg tacaggttgg actcggctgg ggtcgaagta
      721 ccgaccccgc ttgcctttcc ggatgacaac gtgctcgatg aacagaccga cgagggcgcg
      781 ttgctggtct aggtcgaggc ggtcccagct gtcagcagtt acgccctcga aggccgtgcc
      841 gtcgaggttg gggtcgacgg tcagctggtc gagtcgtcgc ctcgccagca gtaggcgagc
      901 gtggatcgga tcctgtgccg caaggaactg cgcacggttg atccgctggg cgccgtagtc
      961 ggcggcgagg tcggcgagga actgttcgtc ggtctcgatc gaggcgacca actggtgggc
     1021 gtcgccgccc gcgcgacgag cgcgggcggt tgcgatgtcg gtgtgttcga gtgcggcgag
     1081 cacgtagtcg acgacgatct gttcgacgta gtcggccttc accgtgttgg agccacaacc
     1141 gggacggttg gccttgtcgc atgcgagcgt ccgccggggc ggccgttgcc ccggcttgag
     1201 ctgcttgtcc atgcgctcgc tcacggcggc catcactgcg ccgcattcgg cgcaacggca
     1261 catcccggcg aggaggttga cctttggctg gcgaccgatg ggcgcatgcg cgaggcggga
     1321 acacacctgc tcccacgtcg gccggtcgag gatcggctcc cagttcgcct cgccgaccac
     1381 cgtgcccttc gactcccgta gtcccgtgat ccgtcccgac ttgaggatca tgcccagcga
     1441 gccgggtcgc cacttcttgc cgtgcgtaga gggggtgcgg cgctcgttga ggtcgacgac
     1501 gatcgacgcg agcgactgtc cacccagcac gcgggctgcg gcctctcgaa ggacgtcggc
     1561 ctcctccggc acgatctcga tcctgccctg tgccactcgc cggtagccgt aggagcggcc
     1621 gccgccgtgg gcgttgccgc gttccgcgtt ctggtggtgc ttggcgctga ctcgttcacc
     1681 gatacgagcg aactcgtgct cggccgctgc accgaggacc tgggcggtga accggccgct
     1741 cgcggtgttg aggtcgacgt gaccggcagc gaccgtgcgg atctcgacgc cggcggaagc
     1801 gaccaccttg gtcagctcga cgaggtcggc gagccgtcgg tagagccggt cggggtggta
     1861 ggtgacgatc acgtcgatct cgcgccggcg cacgaggtcg agcaggcgct cgtactcggg
     1921 gcggcacgca ccggagtagg cgctcaggtc gttgtcgacg agcacctcgt cgacgctcca
     1981 gccctcgagg tcggccagat ggcgacacga gtcgatctgt cggctgacac cagccgcctc
     2041 gccaaccttg tccaacgaga tccgggcgta gatcgcagca cgcccgaaca acgcacctgc
     2101 ccctgtgcgg gcgttcgact gtcgcttcgc caccattcgc tgcgctggct ccttggctgt
     2161 tcgggactcc cttctggagt cccacctgtt ggggccgctc agcgcgacgt gattaccgca
     2221 gaaatttcga ggtcgtgttg tcggcgagca ccttgccgtt ggtttggcag gtggggcagt
     2281 aggcgacggt gtagctgctg taggcgaccg agcggatggt gtcgccgcag acgttgcagg
     2341 gctcgccgat gcggccgtgg acgttgccgg ggcggtcctt ggacgagctc atgtcggtgc
     2401 gggtgcgctc gtaggcgagc ccatcggtga cgcaggcgtg gatcgccgta acgaccgcgg
     2461 cgacgtcgtc ggcaccgagc ttgcccgtgt tggcgaaggg cgacaacttg gcccggtggc
     2521 agacctcgtt cgccagcatg cggccgaggc cggcgatcat gtgctggtcg cgcaggaacc
     2581 cgtgcagccg catggggaac tcacgcatcc gctgttgcat cgccgcggtg tcgaggtcga
     2641 gcgcgtcggg gccgagcttg ctcaacggca gatcggtgag cgcggttgct gtgggcacgc
     2701 accacacacc cgctcggcgc tcggtgccct gttcggtcag cagcaacgcg gcatcggggg
     2761 agccatcctc gggtggctcg aagttgaacc gggcttggcc accgcgcggc ttggcggatt
     2821 gcttggtgtc gaccaacaac ctcccgccct gcatcaggtg gacgacgaag gtcaccggtt
     2881 cgaattcgag cagcaggtac ttgccgcggc ggccgacccg ctgcaacgcc aggccgtagg
     2941 catcgctcgg ctgggggaca gcggtcttga gcgcggtgaa cgtcagcggc gtgaaccggc
     3001 gcagcacccg acccgcgaac gcctcggtca gccgctcggc gtgggcttgg acttcgggca
     3061 gttcgggcac ggctcgctcg cgtcaggtgt agtcggtgcg ttcgcgtacg cggtcgaaga
     3121 tgggtgacag cgcggcgaag accggctggc tgtccatcaa cttctgctga tcgccggtca
     3181 gcaggatgcg accctggatg aacgcctctt gcgcgttgag cgcgccggtc gccacgccga
     3241 cggccgtctc ccacgtctgt tggaagcgaa cgtcttcggg gaacgcctga ccggcggtga
     3301 aggtgaacgc accgtcgccg gacgactgga gcgagtagat cacggtgcct tcggggccgt
     3361 cggtgaccac ctgggtgacg ccgaccgtgc actcttccgc ggcgtcgcgc acggcatcac
     3421 tgactgcagc ttcggccgcg accgcctcga tccactccaa gctcagataa cgcacccccg
     3481 caacgctaat ccgccaacct ggtcgcggtg cggcctcacc ccatcgagcc ccgctgtatc
     3541 cttggtccgt cgcgtccgtc gcggcgcagg attgcgacct gagggctagc gtcgccacca
     3601 tgccaggcga gggtgtctct gcaggccggg tctctgctgg cgagatctct gccgaacgtg
     3661 ttcctgctcg cggggtgcgc gctgagacgg atgggttctt ggtcgtggac gaacagcgcc
     3721 acacggatgc gcaggtcgag gcatggtggt tctggggtgc cgacgaaacc ggcgaggccg
     3781 gggtgtacgc cgggctggaa ctgcgtggcc ggcgggtcga ctactgggcc gggatggtgc
     3841 ggactggatc gccctacttg tacgtggaag aactcgacag tgcctcgctg cgccaaggcc
     3901 tggagatcaa gccgcccgag atgtgggccg gacaccactg cgacgtgccg tttcgccagt
     3961 ggagcgtggg caacgaggcc cacggcgtgc tgctcgacga tccgaccgag gcgtggcggc
     4021 gggcgtacgg cttgccggtg ccgaccacgt tcgacatcga gtggtacggc gagcccgaca
     4081 ccgttgcacc gctcgctggc cttgcccccg gcgcccgcgg ctaccgccag accggctcgg
     4141 tcgacgtcga gatcgaactg ctcgaagggg tgctgcgttt cagcggaccg ggtcagcggg
     4201 tgcacgtctg gggccaggcg tatctgccaa catcgttcgc catgcccatc gatgtgcacg
     4261 gtttgcgcgc gccctaccgc cgcagcgacg gccgcctggt cgaccaggtg ttgaccgatc
     4321 gctggtgggt ctcggtacgt gatctttcgg cgggggaggc tcggccgtga cccgcgacat
     4381 cctcaccgaa ctcgatgccg ccaagcgggc gccgaagtat cgccaggtgc cggtgagcat
     4441 cgggatgatc gtcgaagacc gggccagtgg cttctgcgga gacgtcgtca aggtcagctt
     4501 cgaagcgatc accctgcgcg atcgcaagaa ccagttccgc cacttccgct tcaaggaggg
     4561 cgcgttcttg ttggagggca agccggtcac gctcgtgcgc gacgccgcgc ccgccgccgc
     4621 gacgtccgcg ccgaagctga ccaacagcgg ttcgatcgcg gcgtccggcc cgcaacgggc
     4681 caaggtcgcc aaggctgcgc gcatatgggt cgagggcaaa cacgacgcgg agctggtcga
     4741 acacgtgtgg ggcgacgacc tgcgcgagtt gggcatcgtg gtcgaaccgc tgcacggcat
     4801 cgacgacctc gcgaaagcga tcgatccagc gcccgatctc gtcgggacgg gttttcaatg
     4861 ccgtgcgcga cttccagccc gcgcccacgc gacggctcgg tgtcttggtc gaccatctgg
     4921 tggccgggtc gaaggaggct cgcatcgcgg ccgcggtgcg cgacccgaat gtgctcatca
     4981 cgggccaccc gttcgtcgac gtgtgggcgg gtattcgccc caaggtgatg gggctgacgg
     5041 cgtggcccga cgtgccccgc ggtcaaccgt ggaaggaggg gatgtgccgc gccctcggca
     5101 ccgacctggc cacgttctgg ccaacgctgc gcaatcgcgt caccacctac gccgacctga
     5161 cccccgagtt ggttggtgcc gtcgaacgcc tcatcgactt cgtcgccgac acccccgatc
     5221 cgtgatgggc ggtcagcgcc acatcgcagg gatgagttgc cttccgcggt gagcgagctc
     5281 acccaggatg tgaagcaccc gacgctccga cggcctcgac agggttcagc cgagggcggc
     5341 gttggacact tgggcggcga gggcgatcat gtcggggtcg tgggcgttgg cgacgaggtt
     5401 gagggtgatg ccgtcgaggc cgtgggcgat caggttgctg agttgttcgc cgacctcgtc
     5461 cggcccgccg agggtcatgc gcgggagcat cagatcgaac accgaagcag gccatccgtg
     5521 nnnnnnnnnn nnnngaactc gtgggccgcg gcgaccgcct cttcgtgggt gttgccgatc
     5581 accatcatca ccaacttcgt gacgacgatc tccgaccgat cacgaccgag gttggcgcaa
     5641 tgctcggcca gcacgtcgag cttgcgcgga atgtcctccg gctgcgacgt caggttcgac
     5701 tcgtcagcgt attgggccac catccgcagc gtcttgcgtt cgccgccgcc gccgatcatc
     5761 accgggatct tcgataccgg cgccggcgag ttgatcgcct cggtcacgtg atagtgcgtg
     5821 ctgtgcagcg tcggacgctc gccgcgcagc atcggcagga tgatctgcag cgcctcctcc
     5881 aacttctcga accgggtcga gaacgtgtcg aaggggatgc cgaacgagtc gtgttccagt
     5941 tggaaccatc cggcgccgat gccaagcgtg gcccggccac cggagacgtg gtcgagcgcg
     6001 gtgacgatct tggccagcac ggccgggttg cggtaggtgt tgccggtgac cagcgccgcg
     6061 agccgcgccg ttttggtgtg ttgggccagc gcggccagcg tggtgtagca ctcgagcatg
     6121 taatcgtccg ggtcgccgag gcccggcagt tggtagaagt ggtccatcag caggatgcgg
     6181 tcgaacccag acgcctcggc ggcctgtact tggcggacca gattggggaa gatctgatcc
     6241 ggggcgacac ctggataggt gaagttgggg atctggtagc caaggcgggt catgggcgac
     6301 tccctctcga gatgtggcgg gcacgtgatg taccggccgc gaccgtagcc acatcgtggt
     6361 cagccatcgt gcagcggacc acgtgcagca catcgcctcg tggaatggag cgcagaacgg
     6421 aagcgaaaac ggaacgagcc cgggcgtttc cacccgggct cgtcgaacag cggaacgtgc
     6481 tgtgtcaggt cagtgtgcga ctgccttggc gggggtggaa ccgccgccga tggtcccctt
     6541 ctgacnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6601 nnnnnnnnnn nncgtcgttg tcctgcaggc tgatgatggc cggcagcacc agcagcgaca
     6661 ccaggttcat caccttgatc aaggggttca gcgccgggcc ggcggtgtcc ttgaacgggt
     6721 cgcccaccgt gtcggcgatg accgcggcgc ggtacgggtc gctgcccttg ccgccgtggt
     6781 gaccatcttc gatgtacttc ttcgagttgt cccaggcacc accggcgttc gacaggtagt
     6841 tcgccatgag ctggccgacg aggatgacgg cggccaagaa cgcaccgagg gcgtagaagt
     6901 tgatgccgaa gccgatgatc accggcgtca gcacggcgag cagcgccggg gtggccagct
     6961 cacgcagcga cgccgcggtg cagatg
//
LOCUS       DAORNF010000068         6976 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_8035_length_6976_cov_1.648028, whole genome shotgun sequence.
ACCESSION   DAORNF010000068 DAORNF010000000
VERSION     DAORNF010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 6976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6976
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8035_length_6976_cov_1.648028"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>275)
                     /locus_tag="PLV68_04050"
     CDS             complement(<1..>275)
                     /locus_tag="PLV68_04050"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRE00446.1"
                     /translation="GRMQSERLRDRLAATGEIVADAIVSSQFPRARETAEIVAPALGG
                     LPVVEDERFGEHDPGEECDGMLFSEFIDRFGELNWEADPYAVGFPGG"
     assembly_gap    276..320
                     /estimated_length=45
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            507..>1064
                     /locus_tag="PLV68_04055"
     CDS             507..>1064
                     /locus_tag="PLV68_04055"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HRE00447.1"
                     /translation="MPTPSSRELRSPHQSERDLFFDRFEALTFDDVVIVPGYSEVLPD
                     AVDTSATFARDIRLAIPLISAAMDKVTEAAMAVAMAREGGIGVIHRNMSISDQAAEVQ
                     KVKRSQSGMITDPVTLPATATLHEAEGLMNRFKFSGVPITDADGRLVGILTNRDIRFC
                     EGSDFDRPVSDFMTRDRLITGTVGTT"
     assembly_gap    1065..1148
                     /estimated_length=84
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1149..2044
                     /locus_tag="PLV68_04060"
     CDS             <1149..2044
                     /locus_tag="PLV68_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HRE00448.1"
                     /translation="LAGLITVKDILKRQEFPNGTRDSHGRLACAAAVGVGPDVEARAE
                     ALAAMGADALCIDTAHGHSAGVIKTIEMLRAGWPDVAIVAGNVVTEEGVEALVAAGAD
                     AIKVGVGAGSICTTRVVSGAGMPQLSAIALTCRRAHQLGIPVIADGGITYSGDVVKAI
                     AAGAETVMLGSLLAGTEEAPGELELFEGRRYKSYRGMGSLGAMQGHGADRYASGQSSG
                     TRKLVPEGIEGRVPYAGRLGDVTYQLIGGLRSGMGYAGAGTLSALRDGARFMRVTTAG
                     REESHPHDVTITKEAPNYQRS"
     gene            complement(2086..>2371)
                     /locus_tag="PLV68_04065"
     CDS             complement(2086..>2371)
                     /locus_tag="PLV68_04065"
                     /inference="COORDINATES: protein motif:HMM:NF017569.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRE00449.1"
                     /translation="RRAAVYRFGDAVAERWMVGRVLLLGDARNNYHASQAWVIKNMRQ
                     KARHVYWLNPEPRSYWNTGDSIVGDYGTHTDGVFECRNLRQLESFVEKLA"
     assembly_gap    2372..2381
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2382..>2921)
                     /locus_tag="PLV68_04070"
     CDS             complement(<2382..>2921)
                     /locus_tag="PLV68_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRE00450.1"
                     /translation="AEIRRRLVADRGVDAMAKTLRKPLPEDVEFMHASRDEMASLKKA
                     LQPLTRKLAARLARKRRHGRKGPLDFRNTVRHSLSYGGVPAEPKFKYPRPSKPELVVI
                     ADISGSVAAFARFTLMLVYALQGQFSKVRSFVFIDGIDEVTSFFKGTEDIQEAIHRVN
                     TEADVVWVDGHSDYGHAFEV"
     assembly_gap    2922..2990
                     /estimated_length=69
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2991..>3383)
                     /locus_tag="PLV68_04075"
     CDS             complement(<2991..>3383)
                     /locus_tag="PLV68_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00451.1"
                     /translation="RAFKTVFEVYFSLRGPEYKLVEGDSELDEMWREMQEMRQQGEGK
                     GGGGGMESLSQEELMALLMKALMNGDQGMMRALAKQAVQRFAGMEPGRPVGGTYYLYR
                     TLRNLDLDGMQQKLMDASRQEVGGELTP"
     assembly_gap    3384..3470
                     /estimated_length=87
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3873..3902
                     /estimated_length=30
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3903..5714)
                     /locus_tag="PLV68_04080"
     CDS             complement(<3903..5714)
                     /locus_tag="PLV68_04080"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HRE00452.1"
                     /translation="MTTVTPSTPKPPTAAASTPMPSKSGSSSSSVPTLRALPQFPDSV
                     LRPGRLRWQQRLRAVAILTVLVAVALAAIVIVRRINTDDGQALRTSVVSTQSVSETYH
                     AVAAIEPVTRAQIAFPVSGTVDAVNVAVGDTVAIGDVLATVATTSLEQDLRDAESTLA
                     SAQLTLNVAVSGQDPSTAGGNSLGGGSGGGIVRGAAFSGSGVQFSFAPLTRGTTTYEF
                     LAVSDDERASARQAIVTAQSNVTAATNAAASARDSAVSLCAAVNVTVDPDDPGASVAA
                     INANVGACQTALDQFVAAQTYVTTAQSVLTAAAAAYDTLLDTWAGELEDSASTTTTTP
                     STTPTTTPDSDDSTVPGATTSVPGDSPSMPSGSGSSGSRPSGSGSFPSGSSGGSGGGS
                     TAEAREPTAAELIAYQADYDAAAQAVEAARQALAQATIVSPIEGTVTAVDLAVGQSVS
                     AASDTQVVVVEGDGGYEATLSVSVEDIERIAVGQAAELVPDGGAAPETGEVVAISLVP
                     DTSGSTVNYRVTVALTAAATDLTSGNIGDVGIVVASGDDVVAVPTSAVTPTANGYTVE
                     VVDATGVVQTVRVQIGATGPEWTEIRGGVEAGQTIVIA"
     gene            complement(5717..>6612)
                     /locus_tag="PLV68_04085"
     CDS             complement(5717..>6612)
                     /locus_tag="PLV68_04085"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRE00453.1"
                     /translation="SLAANDANWTTQLTGTTASWTTIRSRHLLTGRFIDADDQASSAA
                     VVVLGATTATQLFESTDVVDQTVSYNGFQLTIVGVLEDVDAADSDNNDLAIVPLSTYA
                     QRLVGGSSRNSVSNIYIKATGDDALSAAYQEAHNILLNVHGISDPADADFSIASEEAV
                     LQAATTEIYTLTVMLGGIAIISLLVGGIGVMNIMLVSVAERISEIGLRKSLGARPRFI
                     RRQFLVEASILGLLGGITGVALGLAGGKLIPHFTDTRVIVSPSASALAIVIACAIGVI
                     FGVYPASRAARLTPIEALRSE"
     assembly_gap    6613..6635
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6636..6873)
                     /locus_tag="PLV68_04090"
     CDS             complement(<6636..6873)
                     /locus_tag="PLV68_04090"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRE00454.1"
                     /translation="MLGILIGISAVVLTIGIGAGARADVRDSINALGTNLLVVSPGSS
                     TDSSGTRGGFGTASTLTASDAEALTSATAAPDIQA"
BASE COUNT         1017 a         2311 c         2274 g         1026 t
ORIGIN      
        1 cgccgcccgg gaaaccgacg gcgtacgggt cggcttccca gttgagctca ccgaagcggt
       61 cgatgaactc gctgaacaac atgccgtcgc actcctcgcc cgggtcgtgc tcgccgaagc
      121 gctcgtcttc cacgacgggc agtccgccca aggccggcgc gacgatctcg gccgtctccc
      181 gggcccgcgg aaattggctc gacacgatgg cgtcggcgac gatctcgccg gtggcggcca
      241 gccggtcgcg cagccgctcg gactgcatcc gccccnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn ccgatgcgac gagcgacggt ggagttggat tcgccgtgcc
      361 ggacgagaac gaggcgggtc acgggtcgcc agggtaggtg acaagcgtcg acttggctga
      421 cttttcgttc aagccggacg ccccgcggcg ggcagggtcg tagggtgagt gcgcatcccc
      481 gtcgatgcgc cagaaggagg ccgccaatgc ccaccccttc gtcccgagag ctacgttcgc
      541 cccatcagtc cgagcgcgat ctgttcttcg accgcttcga ggcactcacc ttcgacgacg
      601 tcgtgatcgt ccccggctac agcgaggtgc tccccgacgc ggtcgacacc tcggccacct
      661 tcgcccgcga catccgcctc gcgataccgc tgatctcggc cgcgatggac aaggtgaccg
      721 aagccgcgat ggccgttgcg atggcccgtg agggcggcat cggcgtgatc catcgcaaca
      781 tgtcgatcag cgaccaggcc gccgaggtgc agaaggtgaa gcgcagccag agtggcatga
      841 tcaccgaccc ggtcaccctg cctgcgaccg cgaccctgca cgaagccgaa gggttgatga
      901 atcggttcaa gttcagcggg gtgccgatca ccgacgccga cggacgcctg gtcggcattc
      961 tcaccaatcg cgacatccgc ttctgcgagg gcagcgactt cgaccgcccg gtcagcgact
     1021 tcatgacccg cgaccggctg atcaccggca cggtcggcac cacgnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnncc ctcgctgggc tgatcacggt gaaggacatc ctcaagcgcc aggagttccc
     1201 caacggcacc cgcgacagcc acggccgcct ggcctgcgct gccgcggtcg gggtcggtcc
     1261 cgacgtcgag gcgcgggccg aggcgttggc cgcgatgggt gccgacgcgc tgtgcatcga
     1321 caccgcccac ggccactccg ccggtgtgat caagacgatc gagatgctgc gcgctggctg
     1381 gcccgacgtg gcgatcgtgg ccggcaacgt cgtcaccgaa gagggtgtcg aggcgttggt
     1441 cgcggccggc gcggacgcga tcaaggtcgg cgtcggtgcg ggttcgatct gcacgacgcg
     1501 cgtcgtgtcc ggcgcgggca tgccccagct ctcggcgatc gcgctcacct gccgccgtgc
     1561 ccaccaactc gggatcccgg tcatcgccga cgggggtatc acctacagcg gtgatgtcgt
     1621 caaggcgatc gccgccggtg ccgagacggt gatgctcggc tcgctgctcg ccggcaccga
     1681 ggaggcaccc ggcgaactgg agttgttcga aggtcgtcgc tacaagagct accgcggcat
     1741 gggttcactc ggggcgatgc agggccacgg tgccgaccgc tacgccagcg gccagtcgtc
     1801 gggtacgcgc aagctggtgc ccgaaggcat cgagggccgc gtgccctacg ccggtcgtct
     1861 cggcgacgtc acctaccagc tgatcggcgg cctgcgcagc ggcatgggct acgccggtgc
     1921 aggcacgctg tcggccttgc gcgacggcgc ccggttcatg cgcgtcacca ccgccgggcg
     1981 cgaagagagc cacccccacg acgtgacgat caccaaggaa gccccgaact atcaacgcag
     2041 ctagcgcatc tcggcaacgc agctcgcgca tctcggcaac ccaggtcagg ccagcttttc
     2101 gacgaacgac tccagttggc gcaggttgcg gcactcgaac acgccatcgg tgtgggtgcc
     2161 gtagtcgccg acgatcgagt cgccggtgtt ccagtagctg cgcggctcgg ggttgagcca
     2221 atacacgtgg cgggccttct ggcgcatgtt cttgatgacc caggcctggc tggcgtggta
     2281 gttgttgcgc gcgtcgccga gcagcagcac ccgccccacc atccagcgct ccgccaccgc
     2341 gtcgccgaag cgatacaccg ccgcccgccg cnnnnnnnnn ncacctcgaa cgcgtggccg
     2401 tagtcggagt ggccgtcgac ccacacgacg tcggcttcgg tgttgacgcg gtggatcgct
     2461 tcttgaatgt cctcggtgcc cttgaagaag ctggtcacct cgtcgatgcc gtcgatgaac
     2521 acgaacgagc ggaccttgct gaactggccc tgcagcgcgt agaccagcat cagggtgaag
     2581 cgggcgaagg ccgcgaccga gccgctgatg tcggcgatga ccaccaactc gggcttggac
     2641 gggcgggggt acttgaactt gggctcggcc ggcacgccgc cgtagctgag cgagtggcgc
     2701 accgtgttgc ggaagtcgag cggacccttg cgcccgtggc ggcgcttgcg ggccaggcgg
     2761 gcggcgagct tgcgcgtcag cggctgcagc gccttcttca acgacgccat ctcgtcgcgt
     2821 gaggcgtgca tgaactcgac gtcttcgggc agcggcttgc gcagcgtctt ggccatggcg
     2881 tcgacgccgc ggtcggccac caggcgccgg cggatctcgg cnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn aacggggtca
     3001 gctcgccccc gacctcctgg cggctggcgt ccatcagctt ctgctgcatg ccgtcgaggt
     3061 cgaggttgcg cagggtgcga tacaggtagt acgtgccccc gaccgggcgg cccggctcca
     3121 tgcccgcaaa gcgctgcacc gcctgcttgg cgagggcgcg catcatgccc tggtcgccgt
     3181 tcatcagcgc cttcatcagc agcgccatca actcttcctg gctgagcgac tccatgccgc
     3241 caccgccacc cttgccctcg ccctgttggc gcatctcttg catctcgcgc cacatctcgt
     3301 cgagctcgct gtcaccttcg accagcttgt actcggggcc gcgcaggctg aagtacacct
     3361 cgaacacggt cttgaaagcc cgcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ccatcgcgtc
     3481 gaggttctcg gtgaggctca ccggcaatcc ggcgttgcgc agttcgccga cgaagccggc
     3541 catcagatcg agcacccgac catcatgcca gcacggaccg cccagaccgc tgcccggcga
     3601 tctcgacaag tttccgaacg cgtctcgacg ggctgcttcc ggtggtggcg aatcacgccg
     3661 acgcgagcgg tcgttcaccg gcaccatcgc cgtcccggcc cgagcagtgc gggtcgtccg
     3721 ccgcccagcc cggccagccg atcgccgagc gcgctgacgg gcccacagcg ctcagccgcc
     3781 gccggggaac aagcctcctc caccgccgcg accgccgcca ccaccaccgg ggaagccgcc
     3841 gccaggaaag ccggtgttgg tctgggtcgc ggnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 nnggcgatca cgatcgtctg gccggcctcg acgccgccgc gaatctcggt ccactcgggg
     3961 ccggtcgcac cgatctggac ccgcacggtc tgcaccacac cggtcgcgtc gaccacctcg
     4021 acggtgtagc cgttggccgt tggcgtgacg gccgaggtgg gcacggccac cacatcgtca
     4081 ccgctggcaa cgacgatgcc cacgtcaccg atgttgccgc tggtcagatc ggtcgctgcc
     4141 gcagtcagcg ccaccgtgac ccgatagttc acggtgctgc cggacgtgtc gggaaccagc
     4201 gagatcgcga cgacctcgcc ggtctcgggt gccgcaccgc cgtcgggcac gagttctgcc
     4261 gcctgaccga ccgcgatccg ttcgatgtcc tcgacgctca ccgacagcgt cgcctcgtag
     4321 ccgccgtcac cctcgaccac gaccacctgc gtatccgaag cggcgctgac cgactggccg
     4381 accgccagat cgacggcggt gacggtgcct tcgatcgggc tgacgatcgt ggcctgcgcc
     4441 aacgcctggc gagcagcctc gaccgcttgt gcagccgcgt cgtagtcggc ttggtaggcg
     4501 atcaactcgg ccgccgtcgg ttcacgagcc tcggccgtcg agccgccgcc cgatccgccc
     4561 gaactcccgg acgggaacga gccgctgccc gacggccgcg agccactgct gccactgccg
     4621 ctcggcatcg agggcgagtc gccgggaaca ctggtggttg cgcccggcac cgtcgaatcg
     4681 tccgagtccg gcgtcgtcgt cggcgtggtc gacggagtgg tcgtcgtcgt gctggccgag
     4741 tcctccagtt caccggccca cgtgtcgagc agcgtgtcgt acgctgcggc cgcagcggtg
     4801 agcacactct gcgccgtggt gacgtaggtc tgcgccgcga cgaactgatc gagcgccgtc
     4861 tgacaggcac cgacgttggc gttgatcgcg gcaacggacg cgcccgggtc gtcgggatcg
     4921 acggtcacgt tcaccgcggc gcagagcgag accgccgagt cgcgggccga ggcggctgcg
     4981 ttggtcgcgg ccgtgacgtt gctctgcgcc gtcacgatcg cctggcgcgc gctcgcccgt
     5041 tcgtcgtcgc tcacggcaag gaactcgtac gtcgtggtgc cccgcgtcag gggcgcgaac
     5101 gagaactgga caccactgcc gctgaaggcc gcaccgcgca cgatgccgcc accgctgcca
     5161 ccgccgagcg agttaccgcc agcggtcgac gggtcttggc cggagacggc gacgttgagg
     5221 gtcaactgtg cggaggcgag cgtcgattcg gcgtcgcgga ggtcttgctc cagcgacgtc
     5281 gtcgcgaccg tggccagcac gtcaccgatc gccacggtgt cgcccaccgc gacgttgacg
     5341 gcgtcgaccg tgccgctgac cgggaacgcg atctgagcac gggtgaccgg ctcgatcgcg
     5401 gcgaccgcgt ggtacgtctc ggagaccgac tgcgtgctga cgacactggt tcgtagcgct
     5461 tgcccgtcgt cggtgttgat ccgccgcacg atcacgattg ccgccaacgc cacagcaacg
     5521 agcacggtca ggatcgccac agcgcgcagc cgttgctgcc agcgcaggcg gcccggccgc
     5581 agcacggaat ccgggaactg cggcagcgcg cgcagggtcg gcacggacga cgaggatgac
     5641 cccgacttgg agggcatcgg tgtggacgcc gccgcggtgg gcggcttcgg cgtggagggg
     5701 gtcacggtgg tcatggtcat tcgctccgca aggcttcgat cggggtgaga cgggcggcgc
     5761 gcgacgcggg atacacgccg aagatcacac cgatcgcgca ggcgatgacg atcgccagcg
     5821 cggaggcaga cggcgacacg atcacgcggg tgtcggtgaa gtgcgggatg agcttgcctc
     5881 ccgcgaggcc gagcgcgacg cccgtgatgc caccgagcag acccaggatc gaggcttcga
     5941 ccaggaactg gcggcggatg aaccgcggcc gggcgccgag cgacttgcgc agcccgatct
     6001 cgctgatccg ctcggcgacg gagacgagca tgatgttcat cacgccgatc ccgccgacga
     6061 gcagcgagat gatcgcgatc ccgcccagca tcacggtgag ggtgtagatc tcggtggtcg
     6121 ccgcctgcag taccgcctcc tcggaggcga tcgagaagtc ggcgtcggcc gggtcggaga
     6181 tgccgtgcac gttgagcaag atgttgtgcg cctcttggta cgcagccgac agggcgtcgt
     6241 caccggtcgc tttgatgtag atgttgctga ccgagttacg gctgctgccg ccgacgagtc
     6301 gttgcgcata cgtgctcagc gggacgatgg cgaggtcgtt gttgtcggag tcggccgcat
     6361 ccacgtcttc gagcacgccg acgatcgtca gttgaaagcc gttgtagctc accgtctggt
     6421 cgaccacgtc ggtgctctcg aacaactggg tcgcggtcgt cgcgccgagc acgaccaccg
     6481 ccgcgctcga cgcctgatcg tcggcgtcga tgaagcggcc cgtcagcagg tggcgggagc
     6541 ggatcgtggt ccagctcgcg gtcgttccgg tgagctgcgt ggtccagttt gcgtcgttgg
     6601 cggccaggct ggnnnnnnnn nnnnnnnnnn nnnnnccgcc tggatgtcgg gcgcggcggt
     6661 ggccgaggtc agcgcctcgg catcactggc cgtcagggtc gaggccgtac cgaacccgcc
     6721 gcgggttccg ctgctgtcgg tcgaactccc cggtgagacc accaacagat tggtgcccag
     6781 ggcgttgatg ctgtcgcgca catccgcgcg cgccccggcg ccgatgccga tcgtcagcac
     6841 cacggcgctg atcccgatca ggatgcccag catcgtgagc accgagcgca agcggtgcga
     6901 gtgcacggcc gaccaggcag agcggagggt gtcacgccac gtcatcgctg ccccaccgcc
     6961 tgggcggccg cgtgac
//
LOCUS       DAORNF010000069         6926 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_8099_length_6926_cov_1.747926, whole genome shotgun sequence.
ACCESSION   DAORNF010000069 DAORNF010000000
VERSION     DAORNF010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 6926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6926
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8099_length_6926_cov_1.747926"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..796
                     /locus_tag="PLV68_04095"
     CDS             <1..796
                     /locus_tag="PLV68_04095"
                     /inference="COORDINATES: protein motif:HMM:NF014698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HRE00455.1"
                     /translation="VEVTGVDLTSEIDEGTADLLRRSLFENCVLIVRDQFLRPADHIR
                     LAMVFGEPFMPHYYAANTLEGFPDIAIVPNFGKLRTPAEGWHTDWSHMTTPPTVSIAI
                     GAVIPPAGGDTMFCNKYATYDRLSDGMKSMLAGRRAKFVGSRPVADTERMHGSADQLP
                     ASRKEAVVNYHPIAREHPGTGRTALYLNRPGSSMAEIEGFTAEESLPILQFLYDQSFT
                     PDNVYRHRWRPGDVVCWDNRCSMHYGVHDYGDVERTLHRITVDGAI"
     gene            808..1323
                     /locus_tag="PLV68_04100"
     CDS             808..1323
                     /locus_tag="PLV68_04100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00456.1"
                     /translation="MKAADLSAVLAINAINAADVDDLTPADMEILFSASTIALAAIDA
                     STGVVGFCVVTDPTCPHRTPRSDWAFDRGADLHLERVAFDMRFSGLGLGLELFTTLDD
                     RIADFVMARPEHEVSPVTLTSIVRSEPPNEHAVRFHASRGFGEVDQRRWDGITYSLMS
                     RSFSDRRTGSF"
     gene            complement(1295..>1528)
                     /locus_tag="PLV68_04105"
     CDS             complement(1295..>1528)
                     /locus_tag="PLV68_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00457.1"
                     /translation="DDMRRLAESIDGFVRWRDVDDGLDYWGVVMFETEEAAIAWRDHP
                     AHVTIHQRSRGELYLTFATMAFERVRTNQFDGR"
     assembly_gap    1529..1597
                     /estimated_length=69
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1623..2504)
                     /locus_tag="PLV68_04110"
     CDS             complement(1623..2504)
                     /locus_tag="PLV68_04110"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRE00458.1"
                     /translation="MSLPIEFVDSHVHFWDHAQTGLTWRYLAADFDHPRLRGMHRLDA
                     PRFTSAELREHAGDRAPTKIVHVQSCEEDRPGMESAWLQSIADADGSIHAIVARARVA
                     EPDLATALDANSRFALVRGFRDMASPLTIGSQSFTDGFDQIADRGLSLEVLVPYPKFE
                     AVCALADRRPEATIVLGHAGLAEHRDPDYFDAWSRHLGMFSTRPNVVVKISALASGAD
                     PNWTTDSIRAWAEWCIDVFGSERAMFASNWPIDRLYGSYERLVDAYLDIATRYTEAQR
                     RDLFHATATRVYRLDQP"
     gene            complement(2501..>4196)
                     /locus_tag="PLV68_04115"
     CDS             complement(2501..>4196)
                     /locus_tag="PLV68_04115"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRE00459.1"
                     /translation="VLATLISGVFALVIGAPALRIRGLFLAVITLAFSVAVSGWLLHQ
                     DWLNNTAADGSSSMRIPRPVIFGVSFESEIRYYWLCLGALVLVALVVYRLRSSGVGRR
                     MIAVRDNEPSAASIAVSPNATKLFAFMISGMIGAFGGFLYGGLLVNFSGDIDGTFGPD
                     QSLSLVVMTVFGGVTTIAGPLLGSVWVQGIPRILGKDWGLLSSGIGVLAVLLALPGGL
                     ASLVFKARDRLVGFAIERHLLTPNPNATPVPSRSVLPRSVPSRSVLEARTTGGDARGA
                     DPDPLEAQNITVRYGGVGALEEVSVRIGASEIVGIMGPNGAGKTTLFDVLSGQQRPDR
                     GAVVFCGADVSTTAPHGRARRGLGRSFQQARLFDDMLLIDAVNLALERHTPTNPVSAI
                     VGTPRARRQNRRRDEEAMAILCDLDLGDAALQQIGGLSTGSRRLAELACASALGAKVI
                     LLDEPTAGLTPQEVRSFTRTIARLREESDVSIVVIDHDVPMMRTLVDRLYVLEAGRLI
                     AHGSPSILDTDERVIEAYMGASRGLTLSKDGSNRRNAPTSATAGTGNETPSEVEHS"
     assembly_gap    4197..4219
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4220..>4858)
                     /locus_tag="PLV68_04120"
     CDS             complement(<4220..>4858)
                     /locus_tag="PLV68_04120"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00460.1"
                     /translation="PVERVSFVIWSITGLLAGLAAILIGPTRPLGLSVALGPFLMLRA
                     LASAMLGGLSGFFAVFAAGLTIGVLENLVVWNYPVGGALDIVLLAAITISFLLKKGLG
                     TAARGAEESSWALAASIKPLHRTIASAARTRAAQYVGIAVLVAIAAFIPTFASVSEQF
                     FLGGVALFGIFGLSLVVLTGFAGQVSLGQFAFVAIGAAFGGRFLQLGFSHLAA"
     assembly_gap    4859..4868
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4869..>5095)
                     /locus_tag="PLV68_04125"
     CDS             complement(<4869..>5095)
                     /locus_tag="PLV68_04125"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00461.1"
                     /translation="FFAAALLPKGGDLAGQRYPTPFNAKLTIGDLVLGPGDLTILVTV
                     PIVALVLATYLGRSKNGRASRASAENSAAARL"
     assembly_gap    5096..5208
                     /estimated_length=113
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5209..5427)
                     /locus_tag="PLV68_04130"
     CDS             complement(<5209..5427)
                     /locus_tag="PLV68_04130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00462.1"
                     /translation="MSLIGLTIPLVDFDIPLTIVIIGLIRGLTYALLGICITLAYRSS
                     RVINFATGEMGALPALLIPIFVLNLGWPY"
     assembly_gap    5586..5595
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5596..5868)
                     /locus_tag="PLV68_04135"
     CDS             complement(<5596..5868)
                     /locus_tag="PLV68_04135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00463.1"
                     /translation="MTRWTLRTQRVVFTSLVTIGLVAGAVLGIREAFIDPVLHPGAPR
                     VRVVDGVATVQVSVRNTSKDTAYCPLVGIAAVDRDGFRGDGRGKGSD"
     gene            complement(5865..6146)
                     /locus_tag="PLV68_04140"
     CDS             complement(5865..6146)
                     /locus_tag="PLV68_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00464.1"
                     /translation="MSNRWWHSPGHSSLLIDELSLGLAPTVTGQLLDVIEQVIELGTT
                     ILLVEQSIGVALAVADTVAFMDRGTIESLGDASSLDAEVLTRRLLEGRS"
     gene            6307..>6926
                     /locus_tag="PLV68_04145"
     CDS             6307..>6926
                     /locus_tag="PLV68_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00465.1"
                     /translation="MPGHHQRDAEPRTLLCGNPDKVLTAVVDVAALCVEQTADVGQQR
                     RLAHPVAPDESMATTLGDHKACIEEDLNRPVREVQTLHLEQWSIGRPPGSHGLRHRQL
                     GGVVDRRVLDGVCPGRRHRGEVVLAHLRAHPALVSAVGRQPTHQQDESGQCGHRRQDE
                     KRCLQARGVGDESEDDRKIYEAVEEIFNGIPPSERLNVFGRHEDEI"
BASE COUNT         1271 a         2224 c         2185 g         1021 t
ORIGIN      
        1 agtcgaagtc accggtgtcg atctgacctc cgagatcgac gaaggtactg ccgatctgct
       61 gcgccgatca ctcttcgaga actgcgtact gatcgttcgc gaccagttcc tccgacccgc
      121 cgaccacatc cgcctcgcca tggtcttcgg cgagccgttc atgccgcact actacgcggc
      181 caacacgctc gaaggcttcc cggacatcgc catcgtcccc aacttcggca agctgcgcac
      241 cccggcggaa ggctggcaca ccgactggtc gcacatgacg accccaccga cggtgtcgat
      301 cgccatcggt gcggtcatcc ccccagctgg gggcgacacg atgttctgca acaagtacgc
      361 gacctacgac cgactctccg acggcatgaa gtcgatgttg gcgggcagac gggcgaagtt
      421 cgtcggcagc cgcccagtcg ccgacaccga gcggatgcac ggcagcgccg accagctccc
      481 cgcgtcacgc aaggaggccg tggtcaacta tcaccccatc gcccgcgaac acccgggcac
      541 gggacgaaca gcgctctacc tcaaccgacc gggcagctcg atggccgaga tcgaggggtt
      601 cacggccgag gagagcctgc cgatcctgca gttcctgtac gaccagtcgt tcacccccga
      661 caacgtctac cggcatcgct ggcggccggg cgacgtggtg tgctgggaca accgctgctc
      721 gatgcactac ggcgtgcacg actacggcga tgtcgagcgc acgctccatc gcatcacggt
      781 cgacggcgcg atatgaccgt acgtgccatg aaggcggccg atctctcggc ggtgttggcg
      841 atcaacgcca tcaacgcggc cgacgtcgac gacctgacgc cggccgacat ggagatcctg
      901 ttctccgcca gcacgatcgc cttggccgca atcgacgcct cgacgggcgt agtcggcttc
      961 tgtgtggtga ccgatccgac gtgcccccac cgaacaccgc gttcggactg ggccttcgac
     1021 cgcggtgctg acctccatct cgaacgcgtc gcattcgaca tgcggttcag cgggctcggg
     1081 ctcgggctgg agcttttcac gaccctcgac gaccgcatcg ccgatttcgt catggctcga
     1141 ccggaacacg aggtgagccc ggtcacgttg acctcgatcg tgcgcagcga accgcccaac
     1201 gaacacgccg tccgcttcca tgcctcgcgc gggttcggcg aagtggacca acgccgatgg
     1261 gacgggatca cctactcgct gatgagcaga tcgttcagcg accgtcgaac tggttcgttc
     1321 tgacccgttc gaaggccatc gtggcaaacg tgaggtacaa ctcgccgcgc gagcgttggt
     1381 gaatcgtgac gtgggcaggg tggtcgcgcc acgcgatcgc ggcctcctcg gtctcgaaca
     1441 tcacgacacc ccagtagtcg agaccatcgt cgacgtcgcg ccaccgaaca aaaccgtcga
     1501 tcgattcggc gagtcggcgc atgtcgtcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncat cagtggtcgg gttcgcagag
     1621 ggtcagggct ggtcgagccg atacacgcgc gttgccgtcg catggaacag gtcgcggcgt
     1681 tgcgcttctg tgtagcgcgt cgcgatgtcg aggtaggcgt cgacgagccg ttcataggag
     1741 ccgtagagac gatcgatcgg ccagttgctg gcgaacatcg cccgctcgga tccgaacacg
     1801 tcgatgcacc actcggccca ggctctgatg ctgtcggtcg tccagttggg gtcggcaccg
     1861 ctggcgagcg cggagatctt gacgacgacg ttcggtcggg tcgagaacat gccgagatgg
     1921 cgggaccagg catcgaagta gtcgggatcg cgatgctcgg ccaggcccgc gtgtcccaac
     1981 acgatcgtcg cctccgggcg ccggtcggcg agggcacaca ccgcctcgaa cttcggatac
     2041 gggacgagca cttcgaggct gagtccgcgg tcggcgatct gatcgaaccc gtcggtgaac
     2101 gactgcgagc cgatggtcag cggcgacgcc atgtcgcgaa acccgcggac caacgcgaag
     2161 cgactgttcg cgtcgagcgc ggtggcgagg tccggctcag cgacacgagc ccgagcgacg
     2221 atggcgtgga tcgagccgtc cgcatcggcg atcgactgca accacgccga ctccatgcca
     2281 ggccgatcct cctcgcacga ctgaacgtga acgatcttgg tcggggcccg atccccagcg
     2341 tgttcacgga gctcggcgct ggtgaagcgg ggtgcatcga ggcggtgcat gccgcgcagg
     2401 cgcggatggt cgaagtcggc agcgagatac cgccacgtca gcccggtttg ggcgtggtcc
     2461 cagaagtgga catggctgtc gacgaactcg atcgggaggc tcatgagtgc tccacctccg
     2521 atggcgtttc gttgccggtt ccggcggtcg cgctcgtggg tgcgttgcgc cgattcgacc
     2581 cgtccttcga cagcgtcagc ccgcgcgacg cgcccatgta ggcctcgatc acccgctcgt
     2641 ccgtgtcgag gatcgacggc gatccatgcg cgatgagacg gccggcctcg agcacgtaga
     2701 ggcggtcgac gagtgtccgc atcatcggca cgtcgtggtc gatgacgacg atggaaacgt
     2761 cggactcctc acgaaggcgg gcgatcgtgc gcgtgaacga acgcacctcc tgtggcgtca
     2821 gacccgccgt tggttcgtcg agcaagatca ccttggcccc gagtgcggac gcgcaggcga
     2881 gctcggcaag ccggcggctg cctgtcgaga gaccgccgat ctgctgcaac gctgcatcgc
     2941 cgaggtcgag atcgcacagg atcgccatgg cttcttcgtc gcggcggcgg ttctggcgcc
     3001 gggcgcgtgg cgtgcccacg atggcactga ccggattggt cggcgtgtgc cgctcgagtg
     3061 ccaggttgac ggcatcgatc aacagcatgt cgtcgaacag ccgagcctgc tggaacgagc
     3121 gacccaggcc gcggcgggcc cggccgtgcg gcgcggtggt cgacacgtcc gcaccgcaga
     3181 acaccaccgc accccggtcg ggtcgctgtt ggcccgacag cacgtcgaac agcgtggtct
     3241 tgccagcgcc gttgggcccc atgatgccga cgatctcgct ggcaccgatg cgtaccgaga
     3301 cctcctccag cgcgccgact ccgccatagc ggaccgtgat gttctgggcc tcgagcgggt
     3361 cggggtcggc gccgcgtgcg tcgcctccgg tcgttcgcgc ctccaggacc gatcgagatg
     3421 gaaccgatcg agggagcacc gatcgagatg gcaccggtgt cgcgttggga tttggcgtca
     3481 acagatgacg ttcgatcgca aacccgacca ggcggtcacg cgccttgaag accagcgacg
     3541 caagccctcc tggcagcgcg agcagcaccg cgagcacgcc gatccccgac gacagcagcc
     3601 cccagtcctt gccgaggatc cgcggtatgc cctgtaccca caccgagcca aggagcgggc
     3661 cggcgatggt cgtcacaccc ccgaacacgg tcatcaccac cagcgacagg gattgatcgg
     3721 gaccgaaggt tccgtcgatg tcgccggaga agttgacgag aagccccccg tagaggaacc
     3781 cgccgaaggc accgatcatc cctgagatca tgaacgcgaa gagcttggtc gcgttgggcg
     3841 agacggcgat gctcgccgcg gagggctcgt tgtcgcgcac cgcgatcatc cgccgcccca
     3901 ccccgctgct gcgcaaccgg tacaccacca gtgcaaccag caccagcgca ccgagacaga
     3961 gccagtagta acggatctcg gactcgaagc tcacgccgaa gatcaccggc cgcgggatcc
     4021 tcatcgacga gctgccgtcg gcagcggtgt tgttcaacca gtcctggtgg agcagccatc
     4081 cgctgaccgc gacggagaag gccagcgtga tgacggcgag gaagagccca cggatcctca
     4141 acgccggggc gccgatgacg agcgcgaaca caccgctgat cagagtcgcg agaaccnnnn
     4201 nnnnnnnnnn nnnnnnnnnc gcggcgaggt gcgagaagcc caactggaga aaccggccac
     4261 cgaaggctgc accgatggcc acgaacgcga actggccgag cgagacctgg ccggcgaagc
     4321 cggtgagaac caccagcgac aagccgaaga tgccgaacag cgccacaccc ccgaggaaga
     4381 attgctcgga caccgacgca aaggtcggga tgaacgctgc aatcgcgacc agcacggcga
     4441 tgcccacgta ctgcgcggcg cgcgtgcgcg ccgcagacgc gatcgtgcga tgcaacggct
     4501 tgatcgacgc ggccagcgcc cacgagctct cctcggcgcc gcgagcagcg gtaccgaggc
     4561 ccttcttgag gaggaagctg atggtgatcg cagcgagcaa cacgatgtcg agcgcgccac
     4621 ccaccgggta gttccacacg acaaggttct cgagcacgcc gatcgtgagc ccagcagcga
     4681 acacggcgaa gaagcccgac agtccgccga gcatcgcgga cgcgagcgca cgcagcatca
     4741 ggaatgggcc gagcgccacc gacaagccga gcggacgagt cgggccgatg aggatcgcgg
     4801 ccagaccggc caacagcccc gtgatcgacc agatgacaaa cgagacccgc tcgacgggnn
     4861 nnnnnnnnag gcgtgccgcg gccgagttct ctgccgaagc ccggctcgcc cgcccgttct
     4921 tgctgcgtcc gaggtacgtc gccagcacga gcgccacgat cggcaccgtg accaagatcg
     4981 tgaggtctcc cggcccgagg acgaggtctc cgatggtgag cttcgcgttg aacggcgtgg
     5041 ggtaacgctg tccggcgagg tcgccgccct tgggcaacaa cgcggccgcg aagaannnnn
     5101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnat acggccaacc
     5221 gaggttgagc acgaagatcg gaatcagcaa cgcaggcagc gcgcccatct caccggttgc
     5281 aaagttgatg acgcgcgacg accgataggc caacgtgatg caaatgccca acagcgcgta
     5341 ggtgagcccg cgaatcagcc cgatgatgac gatcgtgaga gggatgtcga agtcgacgag
     5401 tgggatcgtc aggccgatca gggacacgga ttctccgatt cgatgaacac gacgtactcc
     5461 ttgagctcct cgtcgaactc ctgcggctcg atgttgggca ggacgccgat gaagttgacc
     5521 gaggctctcg gtgcgatccg gccgttgccg aggtctgggg acgcggtcgc agcgtcgagg
     5581 tcaagnnnnn nnnnnatccg accccttgcc ccgcccgtcg cctcggaatc cgtctcgatc
     5641 gaccgccgcg atgccgacca gcgggcagta ggccgtgtcc ttggaggtgt tcctgaccga
     5701 cacctggacc gttgcgacgc cgtcgacgac gcggactcgg ggagctcctg ggtgcaacac
     5761 cggatcgatg aacgcctcgc ggatgccgag cactgctccg gcgacgagcc cgatggtcac
     5821 cagactcgtg aacaccaccc gctgggtgcg aagcgtccac cgggtcatga gcgcccctcg
     5881 agcagacggc gcgtcaacac ctcggcgtcg agcgacgacg cgtcgccaag cgactcgatc
     5941 gttccccggt ccatgaacgc gaccgtgtcg gcgaccgcga gcgcgacacc gatcgactgc
     6001 tcgaccagca ggatcgtggt gcccagttcg atcacttgct cgatcacatc gagcaactgg
     6061 ccggtgacgg tcggcgcgag accgaggctc agctcgtcga tcagcaacga tgagtgccct
     6121 ggcgagtgcc accatctgtt gctcacctcc cgacagggtg ccggcgcgct gcttgagccg
     6181 cttcgccagc gtggggaaga tgccgaggat cgcttcggtc cgctcgtcga tcaggccctg
     6241 ttcggcggtg aatggatatg cgccgaacca gaggttgtcg tacacgctca gtgacgggaa
     6301 cgtcgagtgc ccggccacca tcaacgtgat gccgagccga gaacgctgct ctgcgggaat
     6361 cccgacaagg tcctgaccgc ggtagtagat gttgccgctc tgtgcgtcga gcagaccgcc
     6421 gatgttggcc aacagcgtcg tcttgcccac cccgttgcgc ccgacgagag catggcaacc
     6481 accctggggg atcacaaagc ttgcatcgaa gaggacctga accggcccgt acgagaagtt
     6541 cagaccctgc acctcgagca gtggtccatc ggtcggccgc caggctcgca cggcctccga
     6601 caccgacagc tcgggggcgt cgtcgaccga cgggttctcg acggtgtctg ccccggtcga
     6661 cgccaccgtg gcgaagtagt cctggcgcac ctgcgcgcgc accctgcgct cgtctcggcc
     6721 gtaggtcggc aacccaccca ccagcaggac gagtccggac agtgcggcca caggcgacag
     6781 gatgagaaac gctgtctgca ggctcgtggc gtcggagatg aatccgaaga tgatcgaaaa
     6841 atttacgaag ccgttgaaga aatattcaac ggaattccgc cgtctgaacg cctgaatgtg
     6901 tttggtcgac atgaagatga aataat
//
LOCUS       DAORNF010000070         6877 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_8172_length_6877_cov_1.900322, whole genome shotgun sequence.
ACCESSION   DAORNF010000070 DAORNF010000000
VERSION     DAORNF010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 6877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6877
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8172_length_6877_cov_1.900322"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="PLV68_04150"
     CDS             <1..337
                     /locus_tag="PLV68_04150"
                     /inference="COORDINATES: protein motif:HMM:NF017935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HRE00466.1"
                     /translation="FIRGWFRRYDRCRTCGIAWRREEGFELGAITINTIFTFGTLVVG
                     MTIGLIVTSPDVPVLGLVLALCGIGVVLPVLIYPFTYTLWLALELSVHPPDKAELAQA
                     DLAVDKGAG"
     gene            405..>930
                     /locus_tag="PLV68_04155"
     CDS             405..>930
                     /locus_tag="PLV68_04155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00467.1"
                     /translation="MAIAAPTNPVPADAVCYTRRNVQVAWLVHVLTASGVCVGYLGLN
                     AVNDFRARDAIVWLIAAMLLDGVDGPIARKLAVRERIPHVNGNTLDLVIDYFTCAIVP
                     VAFLDRFDMLPRGTTGVVSFFILGASAIWMSRTDQETPDGWFRGFPAEWNVIIPTLFL
                     ARANPWVTLAVCVVL"
     assembly_gap    931..961
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <962..1183
                     /locus_tag="PLV68_04160"
     CDS             <962..1183
                     /locus_tag="PLV68_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00468.1"
                     /translation="KVEFAHPVSVREQRPVSIAFMTLWLGSMLWLALAERVIQPLRII
                     LVLAPMWTVFQVVRRAVQRRNGTTLRPAG"
     gene            complement(1364..>1796)
                     /locus_tag="PLV68_04165"
     CDS             complement(1364..>1796)
                     /locus_tag="PLV68_04165"
                     /inference="COORDINATES: protein motif:HMM:NF019122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HRE00469.1"
                     /translation="AVATRIQHILGKGVFRLYTNHDVVGCEVGGALKNVVAIACGIAQ
                     GLSVGDNTRSAVMTRGLAELTRLGVAMGGEAATFAGLTGMGDLIATCVSPHSRNRYVG
                     EELGKGRSLDEIVASMAMVAEGIKAAGTAHELALRRRDAGV"
     assembly_gap    1797..1813
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1814..>2225)
                     /locus_tag="PLV68_04170"
     CDS             complement(<1814..>2225)
                     /locus_tag="PLV68_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HRE00470.1"
                     /translation="WGTTMASLVANRHETTLWARNPEVAAEINERSTNDSYLSGFTLP
                     HTLRASSDLAAAVGTAQLLIVAVPSHAFRRTLEDLSAFIHPWIPVVSLSKGFEQGTLM
                     RMTEVINDVLPGHPAAALTGPNLAREIMAGSAAAG"
     assembly_gap    2226..2235
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2612..2717
                     /estimated_length=106
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2718..2904)
                     /locus_tag="PLV68_04175"
     CDS             complement(<2718..2904)
                     /locus_tag="PLV68_04175"
                     /inference="COORDINATES: protein motif:HMM:NF025895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HRE00471.1"
                     /translation="MSSEPFDPASVSVRDNPEQQRFEVYVGDTRAGLADYERNGGTIT
                     FTHTEVDSAFEGRGLAGR"
     gene            2982..>3555
                     /locus_tag="PLV68_04180"
     CDS             2982..>3555
                     /locus_tag="PLV68_04180"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HRE00472.1"
                     /translation="MHLSVLLDMSADALGDRVAVTDRAGGLSYERLRDGARAVATAWS
                     QQGTPERVVFLGTNSRAVPIALFASSLVGAAFTPLNYRLTDAELARIAERAAPAIAVV
                     DDDIAERLAGIDGLTIVPVSEMLAIAEAAPVGIEFDAIPDPDMVGLLLFTSGTTGEPK
                     AAVLRHSHLVSYVFGTLEFASAADGEAGLVS"
     assembly_gap    3556..3565
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3566..4501
                     /locus_tag="PLV68_04185"
     CDS             <3566..4501
                     /locus_tag="PLV68_04185"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase family protein"
                     /protein_id="HRE00473.1"
                     /translation="PPYHIAGVSAIVSSLFTGRRIAYLDSFSPHGWLDAVADQQVTHA
                     MVVPTMLGRVLDEFDNRVRAGWPGVPTLRHLSYGGGRMPVDVIERAVTTWPSVDFVNA
                     YGLTETSSTIAILAPDDHREALASDDPAVRARLGSVGRPIGAVEISIRDEAGEPVPSG
                     VQGEIWVRGEQVSGEYVGRESRATDGWFPTNDAGMLDEAGYLFVFGRLDDVIVRGGEN
                     ISPGEIEEVLRANPHVADVAVFGVPSTEWGETVAAVVVLSDTEPVTDPDELRSFVRER
                     LRSSRVPEIIEYRSELPYSETGKLLRRVLKHELAG"
     assembly_gap    4523..4576
                     /estimated_length=54
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4577..5276
                     /locus_tag="PLV68_04190"
     CDS             <4577..5276
                     /locus_tag="PLV68_04190"
                     /inference="COORDINATES: protein motif:HMM:NF014997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HRE00474.1"
                     /translation="DTTDVTVVAAREVAVFDAPLGSLSRPSWRGRLHVFALPVALVAM
                     TALGVFVADGPRARTAVVIYAVGLCSMFAASATYHRWVHTLRARELWRRTDHAMIYAA
                     IAGTFTPLCLQAVPDRWGLPLLAGVWMLVAASAMLKVVGSGWRHTRIVGGSLYGVISA
                     VAGLAVPALWHRYGVVPALLVIGGGIFYVVGAVGFHRKWPTLRPGVFSYHEVWHAHTV
                     VAAALHFAAVALIL"
     gene            complement(5312..5788)
                     /locus_tag="PLV68_04195"
     CDS             complement(5312..5788)
                     /locus_tag="PLV68_04195"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRE00475.1"
                     /translation="MATRWLSDDEQTAWRAVTSMSARLSARLQRDLQAQSGISEADYA
                     VLVALSEAAEGRLRPYELGRATRWEKSRLSHHLRRMIARGLVERHDCPTDARGAFVTL
                     TPAGHDTIEQAAPLHVESVRAAFVDSLTADELHTFGELAARIAAHLGDDGDCSCDN"
     gene            5954..>6197
                     /locus_tag="PLV68_04200"
     CDS             5954..>6197
                     /locus_tag="PLV68_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRE00476.1"
                     /translation="MLRGHLRPYRSTLLLVTGLQLGQTVASLFLPSINAHVIDKGVRY
                     GDTGYIWRMGSVMLLVALVQIAFAVAAVYFGARTAMA"
     assembly_gap    6198..6207
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6208..>6583
                     /locus_tag="PLV68_04205"
     CDS             <6208..>6583
                     /locus_tag="PLV68_04205"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HRE00477.1"
                     /translation="FGRDVRASLFNQVTGFSSREVGQFGAPSLITRITNDVTQVQMLV
                     QMTCTMLVAAPMMITGGVIMALREDVGLSWILVVAVPVLVAGVSLVIARMVPQFRLMQ
                     ERIDGINQVLREQITGMRVVRA"
     assembly_gap    6584..6593
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6594..>6877
                     /locus_tag="PLV68_04210"
     CDS             <6594..>6877
                     /locus_tag="PLV68_04210"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HRE00478.1"
                     /translation="RDERRRFNAVNDGVTDVAMRAGRLMAFFFPIVVVVLNVSSVAAV
                     WIGADRISDGKMTVGAMMAFLVYLAQILMSVMMGSFIAVLIPRAAVCAER"
BASE COUNT          911 a         2146 c         2359 g         1213 t
ORIGIN      
        1 gttcatccgg gggtggttcc ggcgctacga tcgctgccgc acgtgcggca tcgcatggcg
       61 acgcgaagag ggcttcgagc tcggcgcgat cacgatcaac acgatcttca cgttcggcac
      121 cctggtcgtg ggcatgacca ttgggttgat cgtgacctcg cccgacgtgc cggtgctcgg
      181 cctggtgctg gccttgtgcg gcatcggcgt cgtgctgccg gtgctgatct acccgttcac
      241 ctacacgttg tggctggcgc tcgagctctc ggtgcacccg cccgacaagg ccgaactggc
      301 tcaggccgat ctggcggtcg acaaaggcgc tggctgacca cagtccctcg atcagcggcc
      361 tgggcgcgca caagcgccga tcggggtggc tggggctcta ccctgtggcc atcgccgcgc
      421 caaccaaccc cgttcccgcc gatgcggtgt gttacacccg ccgcaacgtg caggttgcct
      481 ggctcgtcca tgtgctgacg gcgagcggcg tctgtgtcgg ctatctcggg ctcaacgcgg
      541 tcaacgactt tcgggcccgc gatgcgatcg tgtggttgat cgcggcgatg ctgctcgacg
      601 gcgtcgacgg tcccatcgcc cgcaagctgg cggtgcgcga gcgcatccca cacgtcaacg
      661 gcaacaccct cgacctggtg atcgactact tcacctgtgc gatcgtgccg gtggcgttcc
      721 tcgaccggtt cgacatgctg cctcgcggca ccacgggggt ggtctcgttc ttcatcttgg
      781 gggccagtgc gatctggatg tcacgcaccg atcaggagac gcctgacggg tggttccgag
      841 gcttcccagc cgagtggaac gtgatcatcc ccactctgtt cctggcccgt gccaacccgt
      901 gggtcacgct cgcggtgtgc gtcgtgctgt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      961 naaggtcgag ttcgcccacc cggtgtcggt gcgcgagcag cgaccggtgt cgatcgcgtt
     1021 catgacgttg tggctcggtt cgatgctgtg gttggccctg gccgagcggg tcatccagcc
     1081 gctgcggatc attctggtgc tggctccgat gtggaccgtg ttccaggtgg tgcgccgtgc
     1141 cgtgcagcga cgcaacggca ccacgctgcg cccggcgggt tgaatacagc cggttgaata
     1201 cagccggttg aacgccgtcg gttgatcgcg tcggttgccg ccgcccgttg gcgcgcatca
     1261 gcgtgggcgt cagccgggtt gcatctcgtg accggcttgg cggcgcagcc cgttgtaggc
     1321 ctccgacgcg tcgatcgagc cggtgatgac ctggtagatc gattcacaca ccggcatctc
     1381 gacgccgcag cgccaattcg tgggcggtgc ccgcggcctt gatgccttcg gcgaccatcg
     1441 ccatcgaggc gacgatctcg tccaggctgc gccccttgcc gagctcttcg ccgacgtagc
     1501 ggttgcggct gtgggggctg acgcaggtgg cgatcaggtc acccatgccg gtgaggccgg
     1561 cgaaggtagc cgcctcgccg cccatcgcca cgccgaggcg ggtcagttct gccagcccgc
     1621 gcgtcatgac cgcggagcgg gtgttgtcgc cgacggacag accctgggcg atgccacacg
     1681 cgatggcgac cacgttcttc agcgctccgc ccacctcgca tccgaccacg tcgtggttgg
     1741 tgtagagacg aaacacaccc ttgcccaaga tgtgttggat gcgggtcgcg accgccnnnn
     1801 nnnnnnnnnn nnncccgccg ctgcgctacc ggccatgatc tcgcgggcca ggttcgggcc
     1861 ggtcaacgcc gccgctggat ggccggggag cacgtcgttg atcacctcgg tcatccgcat
     1921 cagcgtgccc tgctcgaagc ccttgctgag gctgaccacc gggatccacg ggtggatgaa
     1981 ggcggagagg tcttcgagcg tgcgccggaa ggcgtggctc gggacggcga cgatcaggag
     2041 ttgggcggtg ccgaccgccg cggcgagatc tgacgaggcg cgcagcgtgt gcggcagggt
     2101 gaagcccgac aggtagctgt cgttggtgga gcgttcgttg atctcggccg ccacctcggg
     2161 attgcgggcc cacaacgtgg tctcgtggcg attggcgacc agcgaggcca tggtggtacc
     2221 ccacgnnnnn nnnnncagct cccaagacgg tgatgcgcat cgatcagttc ccctgtgcgg
     2281 tgcggccgcg acgtggagcc gcctcggcgc gagacgttag tccgtcctgt cgccgtgcac
     2341 gtggtggcgt ggcccgcggg tcgtgcaggg tgaaaacggg aaaggccccg ctgggggagc
     2401 ggggcctctc aaccttgacc ctgatgtttg ggggaaacat ctgagggcat catcggagct
     2461 gtttggggga aacttgctcc gagaacgaga ttcagtatgg caccgtctga cctatctgac
     2521 aagcagataa tcaagatact tcctatctct ccgacagata ggcgacggag cgcacgcacc
     2581 ggagagccgg cgctggcggg tcagcgctgg gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnagc gccctgccag accgcggcct tcgaaggcgc tgtcgacctc
     2761 ggtgtgggtg aaggtgatcg tgccgccgtt gcgttcgtaa tcggccagcc cggcacgggt
     2821 gtcgccgacg tacacctcga agcgctgctg ttcgggattg tcgcgcacgg agaccgacgc
     2881 cggatcgaaa ggctcactgc tcatgtgtca ggtctatcgc accgcgaggg cacggcgaca
     2941 cttcttgggg gcgaggccgc ccgatcgctt acgatcgcgc ggtgcatctc tctgtgttgt
     3001 tggatatgtc ggcagacgcc ttgggtgatc gtgtggcggt caccgaccgg gccggtggac
     3061 tgtcgtacga gcgtctgcgt gacggggcgc gagcggtggc caccgcgtgg tcgcagcagg
     3121 ggacgcccga gcgggtcgtg ttcctgggta ccaactcgcg agcggttccc atcgcgttgt
     3181 tcgcttcgtc gctggtcggc gctgcgttca cgccgctcaa ctaccgcctc accgacgccg
     3241 aactggcccg catcgccgag cgcgccgcgc ccgccatcgc cgtggtcgac gacgacatcg
     3301 ccgagcgcct ggccggcatc gacgggctga ccatcgtgcc cgtgtccgag atgctggcca
     3361 tcgcggaggc ggctccggtg ggcatcgagt tcgatgccat ccccgacccg gacatggtcg
     3421 gtctgctgct gttcacgtcg ggcaccacgg gcgaaccgaa ggcggccgtg ctgcgtcatt
     3481 cgcacctcgt gtcgtacgtg ttcggcacgc tggagttcgc cagtgcagcc gacggcgaag
     3541 cggggctggt gtcggnnnnn nnnnnccgcc gtaccacatc gccggagtct cggcgatcgt
     3601 ctcgtcgctg ttcaccggca ggcgcatcgc ctacctcgac tcgttcagcc cgcacggctg
     3661 gctcgacgcc gttgccgatc aacaggtgac ccacgccatg gtggtgccga ccatgctcgg
     3721 ccgcgtgctc gacgagttcg acaaccgcgt ccgcgccggt tggccgggtg tgccgacgct
     3781 tcgccatctg tcctacggcg gtggccggat gccggtcgac gtgatcgagc gggcggtcac
     3841 cacctggccc agcgtcgact tcgtcaacgc ctatggcctg accgagacca gttccaccat
     3901 cgcgatcctc gcccccgacg accatcgcga agcgttggcg tccgacgacc cggcggtgcg
     3961 ggcgcgcctt ggctcggtcg gccggccgat cggcgcggtc gagatctcca tccgcgacga
     4021 ggcgggtgag ccggtgccga gcggcgtgca gggcgagatc tgggtccgcg gcgagcaggt
     4081 gtcgggcgag tacgtcggcc gcgagtcgcg ggccaccgac ggttggttcc ccaccaacga
     4141 cgcgggcatg ctcgacgagg cgggttacct gttcgtcttc ggtcgcctcg acgacgtcat
     4201 cgtgcgcggc ggcgagaaca tctcacccgg cgagatcgaa gaggtgctgc gagccaaccc
     4261 gcacgtcgcc gacgtcgcgg tgttcggtgt gccctcgacc gagtggggtg agaccgtcgc
     4321 tgccgtggtc gtactcagcg acaccgagcc cgtcaccgat cccgacgaac tgcgttcgtt
     4381 cgtgcgcgag cgactgcggt ccagccgcgt gcccgagatc atcgagtacc gcagcgaact
     4441 gccctacagc gagaccggca agctgttgcg gcgggtgctc aagcacgagt tggccggctg
     4501 acgacgagcc gtgccatggc tgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nnnnnncgac acgacagacg tcaccgttgt cgctgcccgc gaggtcgcgg
     4621 tcttcgacgc cccgttgggg tcgctgtcgc gcccgtcgtg gcgtggacgt ctgcacgtgt
     4681 tcgcgctgcc ggtcgcgctc gtcgcgatga cggcgttggg tgtgttcgtc gccgacggcc
     4741 cgcgggcgcg cacggcggtg gtgatctacg ccgtcgggtt gtgttcgatg ttcgcggcct
     4801 cggccacgta tcaccgctgg gtgcacaccc tgcgcgcccg cgagctgtgg cgccgcaccg
     4861 atcacgccat gatctacgcc gccatcgccg gcacgttcac gcccctgtgc ctgcaggcgg
     4921 tgcccgatcg ctggggtcta ccgctgctgg ctggcgtgtg gatgttggtg gcggccagtg
     4981 cgatgttgaa ggtcgtcggg tcggggtggc ggcacacgcg catcgtcggc gggtcgctgt
     5041 acggcgtgat cagcgcggtc gctggcctgg cggttccggc gctgtggcat cgctacgggg
     5101 tggtgccggc gctgctcgtc atcggcggcg gcatcttcta cgtcgtcggg gccgtcggct
     5161 tccaccgcaa gtggccaact ctgcgccccg gggtgttctc ctaccacgag gtctggcacg
     5221 cccacaccgt ggtcgccgcg gcccttcact tcgccgcggt cgcgttgatc ctgtagacgc
     5281 gctcttgtcc gagccgagct ggtgccagcg gtcagttgtc gcaggagcag tcgccgtcgt
     5341 caccgaggtg ggcggcgatg cgcgcggcga gctcgccgaa ggtgtgcagc tcgtcggcgg
     5401 tgagcgagtc gacgaaggcg gcgcgcacgg attcgacgtg caggggagct gcttgttcga
     5461 tggtgtcgtg cccggccggg gtcagggtca cgaaggcccc gcgggcatcg gtggggcagt
     5521 cgtggcgctc gaccaagccg cgggcgatca ttcgccgcag gtggtgagac aaacggctct
     5581 tctcccaccg ggtggcacgg cccaactcgt aggggcgcag gcgcccctcg gcggcttcgg
     5641 acaaggcgac cagcaccgca tagtcggcct cggagatgcc ggactgggct tgcagatcgc
     5701 gctgcaggcg cgccgacaag cgcgccgaca tgctggtgac ggcgcgccac gcggtctgct
     5761 cgtcgtcgct cagccaccgt gtcgccaccc ccgcagccta ggagactgcc cgtcgaatgc
     5821 gacagcgagc accgtggggc ggccggtgcg ccgacgcctc ggtcgccgcg actcggccgg
     5881 atggccgatg ctgcatttcg gtagcggtga gaccggagcg cgccggtagc gtcgctcgac
     5941 aatgctttgg cgcttgttgc gaggtcacct ccgtccctac cgctcgacgc tgctgctggt
     6001 gacggggttg cagcttggtc agacggtggc atcactgttc ctgccgagca tcaatgcaca
     6061 cgtgatcgac aagggcgtgc gctacggcga caccggatac atctggcgga tggggtcggt
     6121 gatgttgctg gtcgcgctgg tacagatcgc gttcgcggtc gccgcggtgt acttcggggc
     6181 gcgcaccgcc atggcgcnnn nnnnnnngtt ttggccgcga cgtgcgggcc agcctgttca
     6241 accaggtcac ggggttctcg tcgcgcgagg tcggtcagtt cggtgcaccg tcgctgatca
     6301 cccgcatcac caacgacgtc acccaggtac agatgctggt gcagatgacc tgcacgatgc
     6361 tggtggctgc accgatgatg atcaccggcg gcgtgatcat ggccctgcgc gaagacgtcg
     6421 ggttgtcgtg gattctggtg gtcgcggtgc cggtgttggt cgctggggtg tcgctggtga
     6481 tcgcgcggat ggtgcctcag ttccggctga tgcaagagcg aatcgacggc atcaatcagg
     6541 tcctacgcga gcagatcacg ggtatgcggg tcgtgcgcgc gttnnnnnnn nnnagcgtga
     6601 cgagcggcgg cggttcaacg ccgtcaacga cggcgtcacc gatgtcgcga tgcgggccgg
     6661 gcggctgatg gcgttcttct ttccgatcgt ggtcgtggtg ctcaacgtct ccagcgtggc
     6721 cgcggtgtgg atcggggccg accggatcag cgacggcaag atgacggttg gggcgatgat
     6781 ggcgtttctc gtctacctcg cccagatctt gatgtcggtc atgatgggca gcttcatcgc
     6841 cgtgttgatc ccgcgggccg cggtgtgcgc cgagcgc
//
LOCUS       DAORNF010000071         6853 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_8218_length_6853_cov_1.602383, whole genome shotgun sequence.
ACCESSION   DAORNF010000071 DAORNF010000000
VERSION     DAORNF010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 6853)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6853)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6853
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8218_length_6853_cov_1.602383"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1140)
                     /locus_tag="PLV68_04215"
     CDS             complement(<1..1140)
                     /locus_tag="PLV68_04215"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00479.1"
                     /translation="MIPESAIMIDNLPAGVEFVSATDGGVYDLSAHRVTWSPAPKFSS
                     SSNGEASVTVRFPADVFSAGAIIVNNATVSGVPYGKTESDRITAPASASHSLSDPVYG
                     RQMFKQVYSSIGRSQTGQFNAPATRGDVAGWNPGVWNRSNVPVDLHVYDTIQPDVGVT
                     SIRYTGHRLILTYTDATEQTFTKLDPTSQQLVTVPVGKTVASIELVREATPPNEQVQM
                     WIDGPVGLGGAATVRNCADSIGRLDGVEVWNTIGVANQQACAGLVIIDTMPIPNVTVS
                     GGPQRLPGQEASWNLYPRNASITTADPVLRELTTITQPRAVIVLGPGHTYVVGSMTCE
                     SAPDPVDRCVVTVSSDGVNQQVTAVWPDRKLLPVDSVAERRIVTAF"
     assembly_gap    1153..1313
                     /estimated_length=161
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1314..2039
                     /locus_tag="PLV68_04220"
     CDS             <1314..2039
                     /locus_tag="PLV68_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00480.1"
                     /translation="EESRPRTVATRFVPSGVKTFLVMTVSVNKPDSPADNVRSVAVVP
                     PPETTSGFAPSEDDRTQPGVPLAWLQLVCTVTTGVSHDSPATPLHEYVTVYVVAFPAR
                     TGVIPGPMDTVKVPPAAATGEELPTRTTRVATNMTADMTANMTVVAAVAPWRPPRRRI
                     VATIDERIAKYELITKESFCPPVCVGDAVSRERLPGDLTHEHRDDDANRAKPSMSGDH
                     GRSRARPPGSLRTWPGNSCTRSG"
     gene            2006..>3157
                     /locus_tag="PLV68_04225"
     CDS             2006..>3157
                     /locus_tag="PLV68_04225"
                     /inference="COORDINATES: protein motif:HMM:NF015413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRE00481.1"
                     /translation="MAGELVYAFRVMRLSLLDAGGSAIGRVEDIVVVPSRPGTPPRVV
                     GFVATSQRRRIFVNANRIGQIDGEGARLRSWDVDLHPFKKKAGELLVGTDILDKKIAD
                     EFVSDVALRPATDGRTSHWELVKVRMGKRVAFRRRPSYRLVDWSEIPGLFGTLTPMAA
                     EAASMRDMHPSDVANVVRTLPLAQRRQLAEAMDDDRLADLLEELPEAEQLRIIEGLDL
                     DRLMKVLDEMEYDDLADLLAEMPGEQRTRILDAMDEDDAAVMRQLLSYEEGTAGGLMT
                     PEIIILGPTATVAEALAMIRDPDWLVSIAAQVFVCRPPFHPPTGQYLGVVHFQRLLRE
                     SPGTELGDCLEHEPTITPDVVDREVAERLASYNMLAVGVTDEAGNLLGAI"
     assembly_gap    3158..3260
                     /estimated_length=103
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3261..3830
                     /locus_tag="PLV68_04230"
     CDS             <3261..3830
                     /locus_tag="PLV68_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HRE00482.1"
                     /translation="DLSAPRQPRRIGLHYDPEAFGQFSESIARTLGTARFLVWQTLLI
                     GLWLTFNYLMPEVFRFDGPEQGFVRLTLILSVQSAYAAPLILLAQNRQEARDRNMTDS
                     DRRVAERTQADTEYLARELASVRLALSGVVTTEDLRDSLAELTAVVEKLSARLDGLDG
                     AGSDAGFSGGVTGGADVGRDAPHELGRQG"
     assembly_gap    3958..3967
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3968..4629)
                     /locus_tag="PLV68_04235"
     CDS             complement(<3968..4629)
                     /locus_tag="PLV68_04235"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRE00483.1"
                     /translation="MYVPWLPPGYTAVLPGRGEMFYRHHQHPDPAAPTVVLLHGWTAS
                     ADLQFFTAYEELAQHVSFIAPDHRGHGRGMRPAEKFRLEDVADDVAALAAALGITKLI
                     AVGYSMGGPIALLLNRAHPDLVAGLVLEATALEWRATRAERLRWKTVGLMSPMMRSWT
                     SPHVMRWALRRVVRRHPALADHVEWVAGEASRNNAVGVVQAGRALSGYDARTWAEALA
                     TPS"
     gene            4702..>4938
                     /locus_tag="PLV68_04240"
     CDS             4702..>4938
                     /locus_tag="PLV68_04240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00484.1"
                     /translation="MFNHWARRLSMVAVLAVRGGSTPSAEPALVAELTALVAEWQRTV
                     DALAAVPTAEIPDADEWAYTLAAATERVEVYQQRL"
     assembly_gap    4939..4973
                     /estimated_length=35
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5282..5746
                     /locus_tag="PLV68_04245"
     CDS             5282..5746
                     /locus_tag="PLV68_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00485.1"
                     /translation="MSLDLDPDFAEFIDCLARHNVRYLVVGGYALAAHGLPRATGDLD
                     IWVMVDPTNASSIVGALDDFGFGGLGITADDFLAHDQVLQLGYPPYRIDVLTAIDGVT
                     FEQAWPRRLTVDSGSVQFSVIGRDDLILNKRAAGRPRDLDDVNRLRGHQPWS"
     gene            complement(5845..>6121)
                     /locus_tag="PLV68_04250"
     CDS             complement(5845..>6121)
                     /locus_tag="PLV68_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00486.1"
                     /translation="QVVHPGVVHSGAVQHWSALVIAAATSSSSPRETSTVARAGQRQP
                     KLRRQRRARQHTRPGDTRRRDKRLEAERDRIATRDPSVVRPSRHRLI"
     assembly_gap    6122..6379
                     /estimated_length=258
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6392..>6853
                     /locus_tag="PLV68_04255"
     CDS             6392..>6853
                     /locus_tag="PLV68_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00487.1"
                     /translation="MRDTHTLTSAATQLKTRASTVAYWLPRLLAAGVIEERGEIKRAG
                     KAMPRYRSVARSFVMSYKQVPIEARIRLFDGGRVDMMNRMLDGLDEELARRPEMRFSV
                     FAPDTATMAITSIDDESTSPKPFTDSWMSLRLDEADAVEFASRLDALCEEFA"
BASE COUNT         1053 a         2137 c         2073 g         1023 t
ORIGIN      
        1 gaaggcggtc acgatcctgc gctcggccac cgaatcgacg gggagcagct tgcggtcggg
       61 ccacaccgcc gtgacctgtt gattgacgcc atcggaggaa acggtgacga cgcagcggtc
      121 gacggggtcg ggggcggact cgcaggtcat ggagcccacc acgtaggtgt ggccgggccc
      181 caacacgatc actgctcgcg gttgggtgat ggtggtgagc tcacgcaaca cgggatcagc
      241 ggtggtgatc gatgcgttcc tcgggtacag gttccacgaa gcctcctgac cggggagccg
      301 ttgcgggcca cccgacacgg tcacattggg gatgggcatg gtgtcgatga tcaccaagcc
      361 agcacaggcc tgctgattgg caacaccgat cgtgttccac acctcgacac cgtcgaggcg
      421 gccgatggaa tcggcacagt tccgcacggt cgccgcgccg ccgagcccga ccggaccgtc
      481 gatccacatc tggacttgct cgttcggcgg tgtcgcttcg cgtacgagct cgatcgaggc
      541 caccgtcttg cccaccggca cggtcacgag ctgttgggac gtggggtcga gtttggtgaa
      601 ggtctgctcg gtcgcatcgg tgtaggtgag gatgagacga tgaccggtgt agcgaatcga
      661 ggtcacgccg acgtcgggct ggatcgtgtc gtagacgtgc agatcgaccg gcacgttgct
      721 tcggttccac acccccgggt tccaaccggc gacgtcgccg cgggttgccg gcgcgttgaa
      781 ttggccggtc tgcgagcgtc cgatggagct gtagacctgc ttgaacatct gccgaccgta
      841 gacgggatca ctgagagagt ggctcgcgct cgccggcgcg gtgatccgat cgctctcggt
      901 cttgccatag ggcacgccgc tgacggtggc gttgttcacg atgattgcgc cagcgctgaa
      961 cacatcggcc gggaaacgga ccgtcacact ggcctcaccg ttgctcgacg acgagaactt
     1021 gggcgccggg ctccacgtga ccctgtgggc actgaggtca tacacaccgc cgtcggtcgc
     1081 cgacacgaat tccacccccg ccgggaggtt gtcgatcatg atcgccgact cggggatcaa
     1141 ccagacagtc ccnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngaggagt
     1321 cgaggccgcg cacagtggcg acaaggttcg tgccgtcggg agtgaagaca tttctcgtca
     1381 tgacggtgag cgtgaacaag ccggactcac cggccgacaa cgtgcgcagc gttgccgtcg
     1441 ttccgccgcc tgagacgacg tcggggttcg cgccgagcga agacgacagg acacagccgg
     1501 gagtgccgct cgcgtggctg cagcttgtct gcacggtgac gaccggcgtt tcgcacgact
     1561 ccccagcgac gccgctgcac gagtacgtca cggtgtacgt ggtggcattc cccgccagga
     1621 ccggtgtgat ccctgggccg atggacaccg tgaaggtgcc tccggcagcc gccactggcg
     1681 aggaactacc aaccaggacg accagggtcg ccaccaacat gaccgccgac atgaccgcca
     1741 acatgaccgt cgtggcggcc gtcgctccat ggcgcccgcc tcgacgtcgc atcgtcgcga
     1801 cgatcgatga acgcattgca aaatatgaac tcattacgaa agaatccttt tgtccgcctg
     1861 tatgtgtggg cgacgcagtg tcccgcgagc gtctgcccgg tgacttgacg cacgaacacc
     1921 gcgatgatga cgccaaccgt gcaaagccgt cgatgtcggg tgaccacggc aggagccgtg
     1981 cgagaccacc cggtagcctg cgcacgtggc cggggaactc gtgtacgcgt tccgggtgat
     2041 gcggttgtcc ctgctcgacg cgggcggatc ggcaatcggt cgcgtggagg acatcgtggt
     2101 ggtcccgtcg cgccccggta ccccaccgcg ggtcgtcggc ttcgtcgcga ccagccagcg
     2161 gcggcgcatt ttcgtcaacg ccaaccgcat cggccagatc gacggtgaag gtgcccgcct
     2221 gcgcagttgg gacgtcgacc tccacccgtt caagaagaag gcgggcgaac tgctggtcgg
     2281 caccgacatc ttggacaaga agatcgccga cgagttcgta tccgatgtcg ccctgcgtcc
     2341 ggccaccgac gggcgcacca gtcactggga gctggtcaag gtgcggatgg gcaagcgggt
     2401 cgcgttccgc cgccggccga gctaccggct ggtcgactgg agcgaaatac cgggcctgtt
     2461 cggcacgctc acccccatgg ccgccgaggc cgcgagcatg cgcgacatgc accccagcga
     2521 cgtcgccaac gtggtccgca ccctgccgct ggcccagcgc cgccagttgg ccgaggcgat
     2581 ggacgacgac cgcctggccg acctgctgga agaactgccc gaggccgagc agctgcggat
     2641 catcgaaggg ctcgatctcg atcgcctgat gaaggtgctc gacgagatgg agtacgacga
     2701 cctggccgac ctgttggccg agatgcccgg cgagcagcgc acccgcatcc tcgacgcgat
     2761 ggacgaggac gatgccgcag tcatgcgcca attgctgtcg tacgaggagg gcaccgccgg
     2821 cggtctgatg acgcccgaga tcatcatcct cggacccacc gccaccgtcg ccgaagcgct
     2881 ggcgatgatc cgcgatcccg actggctcgt cagcatcgcg gcccaggtgt tcgtgtgccg
     2941 ccccccgttc cacccgccga cgggccagta cttgggtgtt gtccacttcc agcggctgct
     3001 ccgtgagtca cccggcaccg agctgggcga ttgcctcgaa cacgagccga cgatcacgcc
     3061 cgacgtcgtc gaccgcgagg tcgccgaacg gctggccagc tacaacatgc tcgccgtcgg
     3121 ggtcaccgac gaggccggga acctgctcgg cgcgatcnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn gacctcagcg cgccccgcca gccgcgccgc atcgggttgc
     3301 actacgaccc ggaggcgttc ggccagttca gcgagagcat cgcccgcacc ctcggcaccg
     3361 cgcggttcct ggtgtggcag acattgctga tcgggctgtg gctgacgttc aactacctga
     3421 tgcccgaggt gttccgcttc gacggtcccg agcagggctt cgtgcgcctg acgctgatct
     3481 tgtcagtgca gtccgcctac gccgcgccgc tgatcttgct ggcccagaac cgtcaagagg
     3541 cccgcgaccg caacatgacc gacagcgacc gccgcgtcgc cgagcggacc caggccgaca
     3601 ccgagtacct cgctcgcgaa ctggccagcg tgcgtctcgc tctcagcggt gtggtcacca
     3661 ccgaagacct gcgcgacagc cttgccgagc tgaccgccgt ggtcgagaag ctgtcagccc
     3721 gcctcgacgg actcgacggg gcgggcagcg atgccgggtt cagtggtgga gtcacgggtg
     3781 gggctgatgt cggccgcgac gcgccgcacg agctcggtcg tcagggctga gaactgcacc
     3841 gagttcatcc acgacgcgag gtggtcgccg tcgagctcgg ccacgtgggc gccgagcgca
     3901 gccgcgagtt gtcgctgctt gcgcggcagc accagccggt cgcgggtggt gaccaacnnn
     3961 nnnnnnngaa ggagtggcca acgcctcggc ccacgtgcgc gcgtcgtagc ccgacaaggc
     4021 ccggcccgcc tgcaccacgc ctacggcgtt gttgcggctc gcctcgccgg ccacccattc
     4081 gacgtgatcg gccagcgcgg ggtggcgccg caccacccgg cgcaacgccc accgcatcac
     4141 gtggggtgac gtccacgatc gcatcatcgg gctcatcaaa ccgacggtct tccaacgcaa
     4201 ccgctcggca cgcgttgccc gccactccaa cgccgtggct tcgagtacca gaccagccac
     4261 caggtcggga tgcgcccggt tgagcaagag cgcgatcggc ccacccatcg agtagccgac
     4321 ggcgatcaac ttggtgatgc ccagcgctgc ggccaacgcc gccacgtcgt cggccacatc
     4381 ttccaaacgg aacttctccg cagggcgcat gccacggccg tggccacgat ggtcgggcgc
     4441 gatgaacgac acgtgctggg caagctcttc gtaggcggtg aagaactgga ggtcggccga
     4501 cgcggtccag ccgtgcagca gcaccaccgt cggcgcggcg gggtctgggt gttggtgatg
     4561 gcgatagaac atctcacccc gacccggcaa caccgccgtg tacccaggcg gcaaccatgg
     4621 cacgtacatg ccgccaggct acggcgcacg tgcccgacgt gaccgcggga ccactcgtgg
     4681 cgacgtcgct aacgtgcaga gatgttcaac cattgggcgc gacgactctc gatggtcgcc
     4741 gtgctcgccg tccgcggtgg ctcgaccccg tcggcggaac ctgccctggt ggccgaactg
     4801 accgcgctcg tcgccgagtg gcagcgcacc gtcgatgctc tcgccgcggt ccccaccgct
     4861 gagatccccg acgccgacga atgggcgtac accctcgccg ccgccaccga acgcgtcgag
     4921 gtctaccaac agcgcctcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnccacagc
     4981 gttcgccccc ggcgccctcc cccacccggg ccccggcgac ctgatcactc tccacctcga
     5041 cacccgcccc tgccgcgccc tccagttctg actggcacgt tctcaccggc atgcttgcgc
     5101 cgcacagagc ggcgtaggct gcatcccatg gaatctcctc gcgtacggaa catcgttgcc
     5161 gtcgtcgagc ggaccccttt gcacggcgag cccgatgagg tggcctattg gttgactcgt
     5221 tccgtcgccg atcggattgc tgcagttcag gtactgcggc gccgagtgtt cggagcacaa
     5281 cttgagtctc gacctcgacc cggacttcgc cgagttcatc gactgtctcg cgcggcataa
     5341 cgttcgatac ctcgtcgtgg gcggctacgc cctcgccgcc cacggcctcc cgcgcgccac
     5401 cggcgatctc gacatctggg tgatggtcga cccgacgaat gcctccagca tcgtcggcgc
     5461 actcgacgac ttcggtttcg gtggcctggg aatcacagcc gatgatttcc tggcacacga
     5521 ccaagtcctg caattggggt atcccccgta ccgaatcgat gttctcaccg ccattgatgg
     5581 cgtgaccttt gagcaagcct ggccgcggcg actcaccgtt gacagtggat cagtccaatt
     5641 ctccgtgatc gggcgcgacg acctcatcct caacaagcgc gcagcaggtc gaccgcgcga
     5701 ccttgacgac gtcaatcgac tccggggcca tcagccctgg tcatgagttg ctgggcagcg
     5761 cgcccggcgc cgtcaagcaa acactcgacc cgggtcggat cgactccggg gcattggctc
     5821 tcgtcgtgag gtgaagccac cgggttagat gaggcggtgg cgggagggac gcaccacgga
     5881 ggggtctcgt gtggcgatgc ggtcgcgttc cgcctccagg cgcttgtcgc ggcggcgggt
     5941 gtctccgggg cgagtgtgtt ggcgagcacg acgttggcgg cggagcttcg gttggcgttg
     6001 accggcgcgg gccaccgtcg aggtctcccg cggtgagctg cttgaggttg cggcggcgat
     6061 gacgagtgcc gaccagtgct gaactgcgcc ggagtgaacg acgccggggt gaacgacttg
     6121 annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6361 nnnnnnnnnn nnnnnnnnnc tgatgccgtt catgcgcgac acccacacgt tgaccagtgc
     6421 ggcaacacag ttgaagaccc gcgcatcgac ggtcgcctac tggttgccgc gcctgctcgc
     6481 cgccggtgtg atcgaagaac gcggagagat caagcgggcg ggcaaggcga tgcctcgcta
     6541 tcgcagcgtc gctcgctcgt tcgtcatgtc gtacaagcag gtaccgatcg aggcccgtat
     6601 ccgcctgttc gacggcggcc gggtcgacat gatgaaccgg atgctcgacg gcttggacga
     6661 ggaactcgcc cggcgtcccg agatgcgctt cagcgtgttc gcccccgaca ccgcgacgat
     6721 ggcgatcacc tcgatcgatg acgaatcgac cagcccgaag ccgttcaccg acagttggat
     6781 gtccctgcgc ctcgacgagg ccgacgcggt cgagttcgcc tcacgcctcg acgcgctgtg
     6841 cgaggagttc gcc
//
LOCUS       DAORNF010000072         6831 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_8257_length_6831_cov_1.616883, whole genome shotgun sequence.
ACCESSION   DAORNF010000072 DAORNF010000000
VERSION     DAORNF010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 6831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6831
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8257_length_6831_cov_1.616883"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..670)
                     /locus_tag="PLV68_04260"
     CDS             complement(<1..670)
                     /locus_tag="PLV68_04260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00488.1"
                     /translation="MEGGRDVAAGRKVGALAACLLVLAACSSGGNAAPESTAVLSTTG
                     STSPATSDAPQTSPPPPETSAPSTSTTSTTTTSTAVTTTPTTLVEPPEAKVRAAVDRA
                     IADFSECLLAMPNCDATTLAATRSDPMLARNVARITEWNAAGYSVIDRDQFRYVIEAV
                     ELDDVAGQATVTVCIADGTKLVLADAAPDGSDIIIDDTFVSGREAWDVRRDADGVWRA
                     YDAPA"
     gene            complement(1020..1697)
                     /locus_tag="PLV68_04265"
     CDS             complement(1020..1697)
                     /locus_tag="PLV68_04265"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRE00489.1"
                     /translation="MNSVFVELGGLGEVALSITHHARRPLATDEPDDGATAWESALIA
                     VTDDEVEHRVAHAETFVIHASRLGGWSILDVLDEISRDLASYTELFDDDGEIAAEFVD
                     SYGGGLIVADRVTVDPAYRGRGLGPLLLAHALADLGAGCELAVCTPAPFEQPTGHDFD
                     GGQQVLTDAVAAQRWAQRQEELRALWSRLGFERFRGTDIYTLELFLPTLEEALAQITD
                     SVYGELD"
     gene            complement(1694..>1939)
                     /locus_tag="PLV68_04270"
     CDS             complement(1694..>1939)
                     /locus_tag="PLV68_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00490.1"
                     /translation="RHTYAAMLIAQQAHPRAIMERMGHSTITVTLDNYGHLFPKLDEA
                     LDDALDDVYQASAIGATTPIDARSTVRSFDPPSTQAG"
     assembly_gap    1940..2057
                     /estimated_length=118
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2058..>2380)
                     /locus_tag="PLV68_04275"
     CDS             complement(<2058..>2380)
                     /locus_tag="PLV68_04275"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRE00491.1"
                     /translation="FLEPEQIMDLAREVSKPPARYRRGERRTDGYPEYGLFVRFAAFT
                     GLRAGEIVGLRVKDLQLMRRRVHVHQSASEAYGQLQVVPTKTYERRSVPIPKSLIDDL
                     TVQVA"
     assembly_gap    2381..2544
                     /estimated_length=164
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2545..>2886)
                     /locus_tag="PLV68_04280"
     CDS             complement(<2545..>2886)
                     /locus_tag="PLV68_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00492.1"
                     /translation="DIQKRVTLSGTRWDVRYRDETERQRKKTFDRKIDAQRFANTVET
                     DLVRGDWIDPDRGRERFGEWADSWVATLGDRKPKTRESYESIVSHHLVPRFGRAPIGA
                     IDYPVVLAYIG"
     assembly_gap    2887..2941
                     /estimated_length=55
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2942..3143)
                     /locus_tag="PLV68_04285"
     CDS             complement(<2942..3143)
                     /locus_tag="PLV68_04285"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRE00493.1"
                     /translation="MSATTSEQLLDIAGLAERLGVGERFVRRLVNERRIPFLKIGRHV
                     RFDSGDVEAWISDTRVEPAAPFT"
     assembly_gap    3148..3340
                     /estimated_length=193
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3379..3452)
                     /locus_tag="PLV68_04290"
     tRNA            complement(3379..3452)
                     /locus_tag="PLV68_04290"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3416..3418),aa:Met,seq:cat)
     assembly_gap    3663..3726
                     /estimated_length=64
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3727..5441)
                     /locus_tag="PLV68_04295"
     CDS             complement(<3727..5441)
                     /locus_tag="PLV68_04295"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HRE00494.1"
                     /translation="MNGIWEPTGAQQELINSEGSRFVEACPGSGKTKAIVARYERLAG
                     AAQRRGIALLSFTNAAVDEVTARCSDPAVLAPPNFVGTFDSFINRFVTGPYTASTLGS
                     YPRFIDSWGSIPGATVRTATMQQGIEFELDWFDWNDAGEFELDEQRVSGQYKGALLTA
                     YRSAPAAIDGIAENRRRQLIKERLIISCAASRHIAMRLLSDDATRATWRKLLSSRFSE
                     IIVDEAQDCGAEELRVLQAALDDDVDVTMVADLDQAIYEFRRARPDDVRSFAGQLPQG
                     TSLTGNFRSSAAICLVSGMLRVGAHQDEAIGPNQTNNWPVRVIEFASLGDVAPVIRDV
                     LSEHELSVDDCKVLAHRRRDAASASGAAMSTSTAGHKMLRIARAHLVLRADPDPANWR
                     RAVNGIERLLAEVASPPGSTDLDHITTLTERLGVSDRWLRSAAVRVLHGAKPEIGRTD
                     YTLEVRRIVQGLDWPNSVAFTNMNHQLSTPPQARWDELAIDPPVSIPWGTIHSVKGQE
                     FEAVALVIPQQLMPDADGRTCLDLWEEGIDGESRRVLYVGATRARRLLIVATHTGQAD
                     RVRHLL"
     gene            complement(5438..5656)
                     /locus_tag="PLV68_04300"
     CDS             complement(5438..5656)
                     /locus_tag="PLV68_04300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00495.1"
                     /translation="MFLAQEPRSLTTWNSIVESDDPAACLYKRMRTNSAFIAKGQFVH
                     DLALRIENGDAFECPAYLADAIAAALDR"
     gene            complement(5703..>6831)
                     /locus_tag="PLV68_04305"
     CDS             complement(5703..>6831)
                     /locus_tag="PLV68_04305"
                     /inference="COORDINATES: protein motif:HMM:NF024573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRE00496.1"
                     /translation="EPLRDAQRDLDSSGSRRLATIIEDLHSAADVAAFVDEANAQLRA
                     VEEHDIVTSTTTEIAGYLSGLTDPVRGQQMGLLFRDYKLHRLAAALRVKLSEAGVDLA
                     DIADSGLGYANLLFIATVLLQLRAATEAELTLLLVEEPEAHLHPQLQSVLLDYLREQA
                     AASVRDDTTGPAGRVQVVVTTHSPVTASSVPVQNVVVLRYTRVALPAPEPDGLADALE
                     SAVHTATVAVAVAELCLGEHETRKLDQYLDATKASLLFGTRILLVEGISEAVLLPVLG
                     RRLYPDEEQSRAHRRALSGLTIVPIGSVDFEPYVRLLLGQTPLGHSILDQLVVITDAD
                     PLPLDDDAEEQDGPDVATNPPPAPRVTRLLALAQGDRRPNA"
BASE COUNT         1014 a         2019 c         2106 g         1098 t
ORIGIN      
        1 cggcgggagc gtcgtacgcc cgccacacgc cgtcggcatc gcggcggacg tcccacgcct
       61 cccggcctga gacgaaggtg tcgtcgatga tgatgtccga gccgtcgggc gcagcgtcag
      121 cgagcacgag cttcgttccg tcagcgatgc acaccgtcac ggtcgcctgc ccagcgacat
      181 cgtcgagttc gacagcctcg atcacgtacc ggaactgatc acggtcgatc acgctgtagc
      241 ccgccgcgtt ccactcggtg atccgagcga cattccttgc cagcatcggg tcggagcgag
      301 tcgcggcgag cgtcgtcgcg tcgcagttgg gcatcgccag caggcactcc gaaaagtccg
      361 cgatcgcccg gtcgaccgca gcgcgcacct tcgcctccgg cggctcgacc agcgtggtgg
      421 gtgtcgtggt gaccgctgtg cttgtcgtcg tcgtgctggt ggtagatgtc gacggcgccg
      481 acgtttcagg aggcggtggc gaggtctgcg gagcgtcact cgtcgctggg ctcgtcgacc
      541 cggtcgtcga caggaccgcg gtcgactcag gggccgcgtt tccgcctgaa ctgcacgccg
      601 cgaggacgag caggcaggct gcgagagcgc cgaccttgcg gccggccgct acgtcacggc
      661 cgccttccat ggcatcatct cgtgtcgcat cgttgaaccc ccaacgtacg gacccgctcg
      721 aaaccagggt acggaacctg cgccgggcgt gcaatgggtc gttcgaacca cttcggttca
      781 tgacgtctcc ctgagctgct cgaccgtggc atcgccacgg aagctgaggg aggaacgagc
      841 cacccgccgg gaggttcccc gactcgcgat cagcgggtcg ttctcgacga tacgcgcact
      901 gtcaaccggc tccacacccc cgtacgggaa gcaacccccg atccggtgga aaccggttgg
      961 aaccggcgcc gtgcacccag ccggtgcgga ggcctgtctc gctcgaccct ggggggagct
     1021 cagtcgagtt cgccgtacac cgagtcggtg atctgagcta gcgcttcctc gagcgtcggc
     1081 aggaagagct ccagggtgta gatgtcagtg ccccggaagc gttcgaagcc aagtcgtgac
     1141 cacagggctc gcagttcttc ttgacgttgc gcccaccgct gcgccgcgac ggcgtcggtc
     1201 agcacctgct ggccgccgtc gaagtcgtgg ccggtcggct gttcgaacgg cgccggggta
     1261 cacacggcga gttcgcatcc cgcgccgaga tccgccagcg cgtgggcgag caggagcggc
     1321 ccgagcccgc ggccgcggta ggccgggtcg acggtcacgc gatcggcgac gatcaggcca
     1381 ccgccgtagc tgtcgacgaa ctcggcggcg atctcaccgt cgtcgtcgaa cagctcggtg
     1441 tagctggcga gatcgcggga gatctcgtcg agcacgtcga ggatgctcca cccaccgagg
     1501 cgggacgcgt ggatcacgaa cgtctcggcg tgcgcgacgc gatgctcgac ctcgtcgtcg
     1561 gtgacggcga tcagcgccga ctcccaggca gtggcgccgt cgtcgggctc gtcggtggcg
     1621 agcgggcggc gggcgtggtg ggtgatcgag agcgccacct cgccaagacc gccaagctcg
     1681 acgaacaccg agttcacccg gcctgtgtgc tgggcgggtc gaacgaccgc accgtggagc
     1741 gggcgtcgat cggggtcgtc gctccgatcg cggaggcttg gtagacgtcg tcgagcgcgt
     1801 cgtcgagtgc ttcgtcgagc ttggggaaca ggtgcccgta gttgtccagc gtgaccgtga
     1861 tcgtgctgtg ccccatccgt tccatgatcg cgcgggggtg tgcctgctgg gcgatgagca
     1921 tcgccgcgta ggtgtggcgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnncgg cgacctggac ggtgaggtcg tcgatcaacg acttcgggat
     2101 cggcacggaa cgccgctcgt acgtcttggt cggcacgacc tgcaactgcc cgtaagcctc
     2161 cgacgccgac tggtggacgt gcacacggcg gcgcatcaac tggaggtcct tgacgcggag
     2221 gccgacgatc tcgccggcgc ggaggccggt gaacgcggcg aagcggacga acagcccgta
     2281 ctcgggatag ccgtcggtgc gtcgttcccc gcgccggtag cgcgccggcg gcttggacac
     2341 ctcgcgggcg aggtccatga tctgttcggg ttcgaggaac nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnncaccga tgtaggcgag cacgaccgga tagtcgatcg
     2581 ctccgatcgg tgcccgcccg aaacgcggca cgaggtggtg gctgacgatc gactcgtagg
     2641 actcgcgggt cttcggtttg cggtcgccga gcgtcgccac ccagctgtcg gcccactcgc
     2701 cgaagcgttc gcggccgcgg tcggggtcga tccagtcgcc gcgcacgaga tcggtctcca
     2761 cggtgttcgc gaagcgctgc gcgtcgatct tgcggtcgaa ggtcttcttg cgttgccgtt
     2821 cggtctcgtc gcggtagcgg acgtcccacc tcgtgcccga caaggtcacg cgcttttgga
     2881 tgtcagnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 ncggtgaacg gcgccgccgg ctcgacacgg gtgtcgctga tccaggcttc gacgtcgccg
     3001 ctgtcgaagc ggacgtgccg tccgatcttc aagaacggga tccgccgttc gttcaccagc
     3061 cggcgcacga accgttcccc gacacccagc cgttcggcga ggccggcgat gtccaacagt
     3121 tgctcgctcg tcgtcgcgct catacccnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn aggcctgggc acctatggtg
     3361 accaggcctt ccagttggtg gcgggggcag gatttgaacc tgcgaccttc gggttatgag
     3421 tcactcctga gccgtccagc ccgtgacgtc ccgtgcacgg cgtgcccctg agcaggcgct
     3481 tcgcgtgccg aaccgtacac ctcatgtcaa tggatcaatc tggtgtcacg ccgtccgtta
     3541 gacgttccgt tagacgttcc gctgtccaga cggtcgcagc ccggacctgc tcatgctgtc
     3601 acgcccatcg cgcacctcgc agcgccaaca gtacggcctc tggcctcaac ggggcatgcg
     3661 gcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnagga gatgacgcac gcggtcagcc tggcccgtgt gggtcgccac gatcagtagc
     3781 cgacgcgcgc gggtcgctcc aacgtagaga actcgccggc tctcgccatc gataccttcc
     3841 tcccacaggt cgagacaggt tctgccgtcg gcgtcgggca tcagctgctg cgggatgaca
     3901 agtgcgacgg cctcgaactc ctggcccttc acggagtgga tcgtccccca ggggatcgag
     3961 accggaggat cgattgccaa ctcgtcccat ctcgcctgag gcggcgtcga aagctggtgg
     4021 ttcatgttgg tgaaagcgac ggagttcggc cagtcgagcc cttgaacgat acggcggacc
     4081 tcaagcgtgt agtcggtgcg cccgatctcc ggtttcgccc cgtgcaggac ccggaccgca
     4141 gcggatcgaa gccagcggtc actcaccccg agccgctccg tcagggtggt gatgtgatcg
     4201 agatcggtcg agccgggcgg cgaagcgacc tccgccagca agcgctcgat tccgttcact
     4261 gctcggcgcc agttggcggg gtccggatcg gcccggagca cgagatgagc tcgggcgatt
     4321 cgtagcatct tgtgcccagc agtggaggtc gacatcgcgg ctcccgacgc agatgctgcg
     4381 tcccttcggc gatgcgccag gaccttgcaa tcgtcaacag agagctcgtg ctcgctgagt
     4441 acgtcacgaa tcaccggggc gacatcgcca aggcttgcga actcgatcac cctcaccggc
     4501 cagttgttgg tttgattcgg tccaatggcc tcgtcctggt gagcgccaac acggagcatg
     4561 ccgctgacga ggcagatggc agcactgctt ctgaaattcc ccgtcaacga tgtcccctgc
     4621 gggagctgtc cggcgaaact tctgacatca tccggcctcg cccggcggaa ctcgtagatc
     4681 gcttgatcga ggtcagcgac catcgtgacg tcgacgtcgt cgtccagggc ggcttggagg
     4741 acacggagct cctcggcgcc acagtcctgc gcctcgtcaa cgatgatctc tgagaatcgg
     4801 gagctcaaga gctttcgcca tgtcgctctg gtggcgtcgt cggagaggag ccgcatcgcg
     4861 atgtggcggc ttgccgcaca gctgatgatc agccgctcct tgatgagttg ccggcgacgg
     4921 ttctcggcaa tcccatctat agcggccgga gcggaccggt acgcggtgag cagcgcgccc
     4981 ttgtactgac cggacacgcg ctgctcgtcg agttcgaact ctcctgcgtc gttccagtcg
     5041 aaccagtcga gctcgaactc gattccctgc tgcatcgtcg cagtacgcac cgtcgcacct
     5101 gggatcgacc cccagctgtc gatgaaccgg gggtaggacc cgagcgtcga cgccgtgtag
     5161 gggccggtga cgaacctatt aatgaaggag tcgaacgtgc ccacgaagtt cggaggggcg
     5221 agcaccgccg gatccgagca tcgcgccgtc acctcatcga cggctgcgtt cgtaaacgag
     5281 agaagggcga tgccccgccg ctgggcagcc ccggcgagcc gctcgtatcg agcgacgatc
     5341 gctttcgtct tgcctgagcc aggacatgct tcgacgaacc gactcccctc ggagttgatc
     5401 agctcctgct gagcaccagt tggttcccag atgccgttca ccggtcgagc gcggcagcaa
     5461 tcgcgtccgc gaggtaggcg ggacactcga aggcgtcgcc gttctcaatc ctgagggcca
     5521 agtcgtgaac gaactggccc tttgctatga aggctgagtt ggtgcgcatc cgcttgtaga
     5581 ggcatgccgc gggatcatct gactcgacga tcgagttcca ggtcgtcaac gagcggggct
     5641 cctgagccag gaacacgtct cggaggaccg gctcgttggc ccccttttcg agcaggtcgg
     5701 cctcaagcgt tcgggcggcg gtctccctgt gcgagagcca gcagtcgcgt aactcgtggg
     5761 gctggaggcg ggttggtggc aacgtcaggg ccgtcctgtt cctccgcatc gtcatccagt
     5821 ggtagggggt cggcatccgt aatcaccact agctggtcga gaatgctgtg gccaagaggg
     5881 gtctggccga ggaggagccg gacgtagggc tcgaagtcca ccgaaccgat cggcacgatt
     5941 gtgaggcccg acagcgcccg gcgatgcgct ctcgactgct cttcgtcggg atagagacga
     6001 cgaccgagga ctgggagcag caccgcctcc gagattccct ccaccaacag gattcgtgtc
     6061 ccgaacaaga ggcttgcctt ggtggcgtcg aggtactgat cgagctttct ggtctcgtgt
     6121 tcaccgaggc acagctcggc gacagcgacc gcaaccgttg ccgtgtggac cgctgactcc
     6181 agagcgtccg cgaggccgtc cggttctggc gcggggagtg cgaccctcgt gtagcgaagc
     6241 acgacgacgt tctgcacggg cacgctgctt gcggttaccg gcgaatgcgt cgtgacgacg
     6301 acctggaccc ttcctgcggg cccggttgtg tcatctcgca cagatgcggc ggcctgctcg
     6361 cggaggtagt ccagcagcac cgactgcaac tgaggatgaa ggtgggcttc cggttcttcc
     6421 acgagcagca gggtcaactc cgcctcggtg gcggcacgga gttgcaggag cacggtcgcg
     6481 atgaagagga ggttggcgta ccccaggccg gaatcagcga tgtcggccag atcgacaccg
     6541 gcttccgaca acttcacgcg aagcgccgcg gcaagtcggt ggagcttgta gtcgcggaag
     6601 agcagcccca tctgctggcc gcggaccggg tctgtgagtc cggacagata ccccgcgatc
     6661 tcggtggtcg tggacgtgac gatgtcgtgc tcctcgactg ctcggagctg cgcgttcgcc
     6721 tcatcgacaa acgctgcgac atcggcagcg ctgtgaaggt cctcgatgat cgtcgctaac
     6781 cggcggctcc ccgaagagtc caggtcccgt tgggcgtcgc gcagcggctc g
//
LOCUS       DAORNF010000073         6799 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_8302_length_6799_cov_1.613582, whole genome shotgun sequence.
ACCESSION   DAORNF010000073 DAORNF010000000
VERSION     DAORNF010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 6799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6799
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8302_length_6799_cov_1.613582"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>386
                     /locus_tag="PLV68_04310"
     CDS             <1..>386
                     /locus_tag="PLV68_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00497.1"
                     /translation="GAGAVDPEALALKGEYLKGVLPDLRDNKGDIRQESKEAARAKAL
                     AMAEKLVAKHQIAAHAKELADFDQSLLPVPVRAGKQSGLTKLGKSMGRAFSKSSVKTR
                     RSDKAQQLRKGVDAMDGDQTEQAYKQ"
     assembly_gap    387..396
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <397..779
                     /locus_tag="PLV68_04315"
     CDS             <397..779
                     /locus_tag="PLV68_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00498.1"
                     /translation="MGGGSHRQRQAEFDIGVQHAILAMHRRAVAFENADFDIMKLQAA
                     MSDKGAVAEHLKPYQAVMGAGGYAKAVADFNLLAKGYTDQLSRSGDGEKVREFATASK
                     QIDDNRQGFEAAIDFIDRRLAKTS"
     assembly_gap    804..830
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <831..>1963
                     /locus_tag="PLV68_04320"
     CDS             <831..>1963
                     /locus_tag="PLV68_04320"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HRE00499.1"
                     /translation="AAGLAASISAGAVAAASSPTQSGPATAAASAVRLAPPLRTLSVV
                     ASGDVLTENIVLAAAAREAQPGQRYDFAALMAPIAPAVASADLAICHLELVTGRPGEP
                     AANAGKSQSMGYRLRSPHEVVVGMAQTGFDRCSTASNHSNDLGFGGIVTTLDGLDLVG
                     ISHTGTARSPQEAAPGQQQVVVNGVRVAHLSYTSYSNDGAPSQPWQLNMSISPQRVAG
                     DVRAARDAGAEVVIVSLHTFKEMERAPIPADRRFVEQLTAMVPVDLIVQHGPHVIQPI
                     EWVNGALVYWSIGNLLSGMGVAGRWRYDDMRTVDGLLATVRFTETSPGSFVVSTVPLL
                     VCLDPASRVVWPITALDDPATPPSLVPMLQACRTRSRSVIPGL"
     assembly_gap    1964..1980
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2177..2353
                     /locus_tag="PLV68_04325"
     CDS             2177..2353
                     /locus_tag="PLV68_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00500.1"
                     /translation="MSTTGGCLAVEHAGVDESVLADAIRTNLHGTATYGAEVVMVSID
                     PLVVDLHCCHGAHH"
     gene            2449..3204
                     /locus_tag="PLV68_04330"
     CDS             2449..3204
                     /locus_tag="PLV68_04330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HRE00501.1"
                     /translation="MTDQQYPAASTVEEFADNLAAVRARIDAAARRAGRDPAEVRLLP
                     VSKTVPQERLRLAVAAGCSQLGENKVQEAVRKSREMADLGIAWSVIGHLQTNKARDVV
                     AVAAEFQALDSVRVAEALDRRLQAAGRGLDVFVQVNTSGEESKFGLAPEDLPAFLAEL
                     PNYSSLRVRGLMTLAIFSDDTIAVRGCFTLLRTLRDRARDTDPGLIGPGELSMGMSGD
                     YELAVEEGATCVRVGQAIFGARAVPDSHYWPNP"
     gene            complement(3217..>4814)
                     /locus_tag="PLV68_04335"
     CDS             complement(3217..>4814)
                     /locus_tag="PLV68_04335"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF012946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRE00502.1"
                     /translation="TTGNHIDSTPAARHEWRCGERRYCLHGQQHADGTEENRMSQTTE
                     HFDVLIAGAGISGIGAAYHLQTQVPGKSFVVLEKFGGFGGTWRVHTYPGVRSDSDLYT
                     FGYRFKPWNGAPIAAADKILDYLHEVIDDNDLARHIRYDHEITDASWDSGTNRWTLQV
                     TQTDPDSGEQTRRTFTANFLWMCQGYYRHEQGYTPDWPGFSSYQGQVIHPQTWPDDVD
                     LSGKKVVVIGSGATAATLIPAIAGQCDGVTMLQRSPTYFVSRPNVNELADTLRSLDIP
                     EEWTHEIVRRKNLQDGAAITQLSFEAPELMRDELLRLVQAELPEGFDMRHFTPAYRPW
                     QQRLALVPDGDMFAAIREGKASVVTDEIETFNETGILTKGGVQLDADVVITATGFDLS
                     VLGGIPFSVDGQPVSWPDTVTYRGTMVTGVPNMAWVFGYFRSSWTLRVDLIGDFVCRL
                     LQHMDELGASRVTPALRTEDAEMTISGWIDPDNFNPGYMMRSMHLLPKRGDKPEWQHT
                     QDYEAERVALPAADLDDGCLVFE"
     assembly_gap    4815..4834
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4835..5540
                     /locus_tag="PLV68_04340"
     CDS             <4835..5540
                     /locus_tag="PLV68_04340"
                     /inference="COORDINATES: protein motif:HMM:NF024748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class A beta-lactamase-related serine hydrolase"
                     /protein_id="HRE00503.1"
                     /translation="RAGGAVVGDRWTDVARRFVAGEQGRSVVLTGDECWVGEAADVAR
                     PGASVLKVFVAAAACLDMAAERLDPSESVAVSDLPTSINPSMLESLAPGHRFSLAELT
                     CLSLSTSENRIGEFMVQRVGIGRVNAVAASLGCPGTALRVGFGDEMLSPLGRANVTTA
                     ADCATLLAAIWRRDELRVLRAALRSSLFNARILARMPDDVTVSHKTGSLSGVANDIGV
                     IHSRRLHRLLPDGWTS"
     gene            5613..>5884
                     /locus_tag="PLV68_04345"
     CDS             5613..>5884
                     /locus_tag="PLV68_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00504.1"
                     /translation="MIVHPTVRNGDMVTIRFDAASGPNEITIRVPVDVPVDLGPDPAL
                     AALWMPAMAMREELVFSEPVSPMLLANATTFADIVLRSHGSLDLLV"
     assembly_gap    5885..5938
                     /estimated_length=54
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5939..6757
                     /locus_tag="PLV68_04350"
     CDS             <5939..6757
                     /locus_tag="PLV68_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00505.1"
                     /translation="TVVAPHAASRRVACCFTGGIDSFYSIIAHRDEIDLLVFVHGFDL
                     TDDEHSPLNRSVVARLYEAAAMLGKPMIEVTTGLKYFGAALGIDWNDYLGSAVAAVAL
                     LLAPTVSTFYIPATATYAEMYPLGTHPLLDPLWSTEAVRIVHDGAQATRVGKTRIVAS
                     EPAAQQHLRVCWEHRDGLYNCGRCEKCIRSAVGVRIAGVEGAFPALPSPSLRQIAGVN
                     VVGLGKSWLGYRDELARSGRNPRLRFAIDVALWRWRVKRLKRLKSLPGLRRVWR"
BASE COUNT         1032 a         2159 c         2343 g         1137 t
ORIGIN      
        1 cggtgccgga gctgtcgacc ccgaggccct ggcactgaag ggcgagtacc tcaagggcgt
       61 gctgcccgat ctgcgtgaca acaagggcga cattcgccag gagtccaagg aagcggcacg
      121 ggccaaggcg ctggccatgg ccgagaagct ggtcgccaag caccagatcg ccgcccacgc
      181 caaggaactg gccgatttcg accagtcgct gttgcccgtg ccggtgcgcg ccggcaagca
      241 gagcgggctg accaagctgg gcaagagcat ggggcgcgcg ttcagcaagt ccagcgtcaa
      301 gacccgcagg tccgacaagg cccagcagtt gcgcaagggc gtcgacgcga tggatggcga
      361 tcagaccgag caggcgtaca agcaggnnnn nnnnnncgat gggcggaggc agtcaccgcc
      421 agcgccaggc cgagttcgac atcggcgtgc agcatgcgat cttggcgatg caccgccgag
      481 ccgttgcatt cgaaaatgcc gatttcgaca ttatgaagct gcaagcggcc atgtctgaca
      541 agggcgcggt ggccgagcac ctcaagccgt atcaggcggt gatgggtgcg ggcggctatg
      601 ccaaggcggt ggccgatttc aacctgctcg ccaagggcta taccgatcag ctctctcgct
      661 cgggcgacgg tgagaaggtg cgcgagttcg cgaccgcgag caagcagatc gacgacaacc
      721 gccagggctt cgaggccgcg atcgatttca tcgaccgccg cctcgccaag acctcctgag
      781 tcgaaccgct gcatcgcgcc gcgnnnnnnn nnnnnnnnnn nnnnnnnnnn ggcggctggc
      841 ctggccgcgt cgatctcggc cggtgccgtc gccgctgcat ccagcccgac ccagtccggc
      901 ccggcaaccg ctgccgcatc cgcggtgcga ctggcgccgc cgctgcgcac attgtcggtc
      961 gtcgcgtccg gcgacgtgtt gaccgagaac atcgtgctgg ccgccgccgc ccgcgaagcc
     1021 cagcccgggc agcggtacga cttcgctgcg ctgatggctc cgatcgcgcc cgcggtggcg
     1081 tcggccgact tggcgatctg tcatctcgaa ctggtcaccg gacgtcccgg ggaaccggcg
     1141 gccaatgcgg gcaagtcgca gtcgatggga tatcggctgc gctcgcccca cgaggtcgtg
     1201 gtgggcatgg cccagaccgg cttcgaccga tgcagcacgg cgtcgaacca cagcaatgat
     1261 ctgggcttcg gtggcatcgt caccaccctc gacggtctcg acctggtcgg catcagccac
     1321 accggcacgg cccgctcgcc gcaggaggcg gcacccggcc aacagcaggt cgtcgtcaac
     1381 ggtgtgcggg tcgcccacct gagctacacc agctacagca acgacggcgc accgtcacag
     1441 ccgtggcaac tgaacatgtc gatcagcccg cagcgggttg caggtgacgt gcgagccgcc
     1501 cgcgacgccg gggccgaggt ggtgatcgtc agcctgcaca cgttcaagga gatggagcgg
     1561 gccccgatcc cggccgaccg ccgcttcgtc gagcagttga cggcgatggt gccggtcgat
     1621 ctgatcgtgc agcacggtcc gcacgtgatc cagccgatcg agtgggtcaa cggcgcgttg
     1681 gtgtactgga gcatcggcaa cctgctctcg ggcatgggtg tggccgggcg ttggcgttac
     1741 gacgacatgc gcactgtcga cgggctgctg gccacggtgc ggttcaccga gacctcgccg
     1801 ggcagtttcg tcgtcagcac ggtgccgctg ctggtgtgcc tcgatcctgc gtcgcgtgtg
     1861 gtgtggccga tcaccgcgct ggacgacccg gccaccccgc ccagcctggt tccgatgctg
     1921 caggcctgcc gcacccgctc ccgctccgtg atccccggcc tgtnnnnnnn nnnnnnnnnn
     1981 gggttcggtt ggtttctcta ggcgacgata ggtttatgtg catcgtctgg agtcaggagc
     2041 cgatgtgcca accgacggaa ctgctgccaa ccggcctgtg cgcaccgagt tgcgtgtcga
     2101 cggggccaac tgcccgacct gtttgaacaa caccgcggac gtgctgcgtg cgatccctgg
     2161 cgttcgatcc gtcgagatgt ccacgactgg cggctgcctt gccgtcgaac acgctggtgt
     2221 ggacgaatcg gtgctggccg acgcgatcag gaccaacctg cacggcactg ccacgtacgg
     2281 agccgaagtg gtgatggtgt cgatcgatcc gctggtggtc gacctgcact gctgccacgg
     2341 tgcacatcac tgatgtgctg gtcgtgtcgc ccaacagcga tcgaacagcg ttggaggtag
     2401 cgtcggcggg gtcagtggtg cgcgtggtga ccggcgtgga gaggacgaat gactgaccag
     2461 cagtacccgg cggcgtcgac ggtcgaggag ttcgcggaca atctggccgc ggtgcgggct
     2521 cggatcgatg cggcggcgcg gcgggccggg cgtgacccgg ccgaggttcg cctgttgccg
     2581 gtgagcaaga cggtgccgca agagcggctg cggctcgcgg tcgcggccgg gtgcagccaa
     2641 cttggcgaga acaaggtgca agaggccgtg cgcaagtcgc gcgagatggc cgatctgggc
     2701 atcgcctggt cggtcatcgg ccacctccag accaacaagg cgcgcgacgt ggtcgcggtt
     2761 gcggccgagt tccaggcgct cgacagcgtg cgtgtcgccg aagcgctcga ccggcgactg
     2821 caggcggccg ggcgtggcct cgacgtgttc gtgcaggtca acacgtcggg cgaggagtcg
     2881 aagttcggcc tggccccgga ggatctcccg gcgttcttgg ccgagctgcc caactactcg
     2941 tcgctgcgcg tccgcggcct gatgaccctg gcgatcttca gcgacgacac catcgccgta
     3001 cgcgggtgct tcacgctgtt gcgcaccctg cgcgaccgcg cccgcgacac cgatcccggc
     3061 ctgatcggcc ccggtgagct gtcgatgggc atgtcaggcg actacgagct cgctgtcgaa
     3121 gagggcgcca cctgcgtccg ggtcggccaa gcgatcttcg gcgcccgcgc cgtgcccgac
     3181 agccactact ggccgaaccc gtagcagccg accgggctac tcgaacacga ggcagccgtc
     3241 gtcgaggtcg gcggcgggca gagccacccg ttctgcttcg tagtcctggg tgtgctgcca
     3301 ctcgggcttg tcgccgcgct tgggcagcag gtgcatgctg cgcatcatgt agccggggtt
     3361 gaagttgtcc gggtcgatcc agcccgagat cgtcatctcg gcgtcctcgg tccgcagcgc
     3421 gggcgtgacc cgggacgcgc ccagctcgtc catgtgctgc agcaggcggc agacgaagtc
     3481 gccgatcagg tcgacgcgca acgtccagct actgcggaag tagccgaaca cccaggccat
     3541 gttgggcacg cccgtgacca tcgtcccccg gtaggtcacg gtgtcgggcc agctcaccgg
     3601 ttgtccgtcg acgctgaagg ggatgccgcc gagcacgctg agatcgaatc cggtggcggt
     3661 gatgacgaca tcggcgtcga gttgcacgcc gcccttggtg aggatgccgg tctcgttgaa
     3721 ggtctcgatc tcgtcggtga cgaccgatgc cttgccctcc ctgatcgcgg cgaacatgtc
     3781 gccgtcgggg accagcgcca agcgctgctg ccacgggcgg taggccgggg tgaagtggcg
     3841 catgtcgaag ccttctggca gttcggcctg caccaggcgg agcaactcgt cgcgcatcaa
     3901 ctcgggtgct tcgaacgaca gctgagtgat cgccgcgcca tcttgcaggt tcttgcggcg
     3961 gacgatctcg tgggtccact cctcggggat gtcgaggctc cgcagcgtgt cggccagctc
     4021 attgacgttg ggtcgcgaca cgaagtaggt cggtgagcgt tgcagcatcg tgaccccgtc
     4081 gcactggcca gcgatcgccg ggatcagggt ggccgcggtg gcgccggagc cgatcacgac
     4141 gaccttctta ccggacaggt cgacatcgtc gggccaggtc tgagggtgga tgacctgacc
     4201 ctgatacgag ctgaaccctg gccagtcggg ggtgtagccc tgctcgtggc ggtagtagcc
     4261 ctggcacatc cacaagaagt tggcggtgaa cgtgcgtcgt gtctgctcgc cggagtcggg
     4321 gtcggtctgg gtgacctgca gcgtccagcg gttcgtgccg ctgtcccacg acgcgtcggt
     4381 gatctcgtgg tcgtagcgga tgtggcgggc gaggtcgttg tcgtcgatga cctcgtggag
     4441 gtagtcgagg atcttgtccg ctgccgcgat cggtgctccg ttccacggct tgaagcggta
     4501 gccgaaggtg tacaggtcac tatcggagcg caccccggga taggtgtgca cccgccacgt
     4561 cccaccgaac ccgccgaact tctccagcac gacgaacgac ttgccgggaa cctgcgtctg
     4621 caggtgatag gcggcaccga tgccagagat gcctgctccg gcgatcaaca catcgaagtg
     4681 ctcggtggtc tggctcatcc ggttctcctc agttccgtca gcgtgttgct ggccgtgcag
     4741 gcagtatcgc cgctcgccac aacgccactc gtgtcgagcg gcaggagtgc tgtcgatatg
     4801 gttgcccgtc gtggnnnnnn nnnnnnnnnn nnnnacgtgc tggcggggcg gtggtgggcg
     4861 accggtggac cgacgtggcg cggcggttcg ttgcggggga acaggggcgc agcgtggtgc
     4921 tgaccggcga cgagtgttgg gtcggtgagg cggctgacgt cgcgcggccc ggagccagcg
     4981 tgttgaaggt gttcgtcgcc gcggcagcgt gtctcgacat ggctgccgag cgcctcgacc
     5041 ccagcgagtc ggtcgcggtc agcgacctgc cgaccagcat caatccgtcg atgctcgagt
     5101 cgttggcgcc cggccatcgc ttcagcctgg ctgaactgac ctgcctgtcg ttgtcgacca
     5161 gcgagaaccg catcggtgag ttcatggtgc agcgcgtcgg catcgggcgg gtcaacgccg
     5221 tggccgcatc gctcggttgt ccgggcaccg cgttgcgggt ggggttcggc gacgagatgc
     5281 tcagcccgct cgggcgggcc aacgtcacca cggcggccga ctgcgcgacg ctgctcgccg
     5341 cgatctggcg ccgcgacgag ttgcgcgtgc tgcgcgcggc actgcggtcg agtctgttca
     5401 acgcgcgcat cctggctcgg atgcccgacg acgtgaccgt gtcgcacaag accggcagcc
     5461 tgtctggcgt ggcgaacgac atcggggtga tccactcccg gcgacttcat cgcctgcttc
     5521 ctgacggatg gacaagctga cacggcggtc acgtcgtcag agatcggcca atgtgcgctc
     5581 gacgcaatga tcgcatggga ggctcgctgc gcatgatcgt gcaccccacc gtccgcaatg
     5641 gtgacatggt caccatccgc ttcgacgccg cgtcggggcc gaacgagatc acgatccggg
     5701 taccggtcga tgtgcctgtc gacctcggac ccgacccggc tctcgccgcg ttgtggatgc
     5761 cggcgatggc gatgcgcgag gagctggtgt tcagcgagcc ggtatcaccg atgctgctcg
     5821 ccaacgcgac gacctttgcc gacatcgtgc tgcgctcgca tggcagcctg gacctgctgg
     5881 tcaannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnac
     5941 cgtggtcgcg ccacacgccg cttcgcgccg tgtggcgtgt tgcttcaccg gcggcatcga
     6001 ttccttctat tcgatcatcg ctcaccgtga cgagatcgac ctgctcgtct tcgtccacgg
     6061 tttcgacctg accgacgacg agcacagtcc gctcaatcgg agcgtcgtcg ccaggctgta
     6121 cgaggccgcg gccatgctcg gcaagccgat gatcgaggtg acgaccgggc tcaagtactt
     6181 cggagccgca ctcggcatcg actggaacga ctacctcggt tcggccgtcg ccgcggtcgc
     6241 gctgctgctg gcccccacgg tgtcgacgtt ctacatcccg gccaccgcga cctacgccga
     6301 gatgtacccg ttgggcaccc acccgttgct cgacccgctg tggagcaccg aggcggtgcg
     6361 gatcgtgcac gacggggcac aggcgacgcg ggttggcaaa acccgcatcg tcgcatctga
     6421 accggcagct cagcagcatc tgcgggtgtg ttgggagcat cgcgacggcc tctacaactg
     6481 cggccggtgc gagaagtgca tccgctcggc cgtcggtgtg cgcatcgccg gggtcgaagg
     6541 ggccttcccc gcgctgccgt cgccgtcgct gcgtcagatc gcgggggtca acgtcgtcgg
     6601 gttgggaaag agttggctcg gctaccgcga cgagttggct cgcagcggcc ggaacccgcg
     6661 gctgcggttc gcgatcgatg tcgccttgtg gcgttggcgc gtgaaacggc tcaagcggct
     6721 gaagagcttg ccaggcctgc gtcgcgtgtg gcggtgacgg cggcgtggca tgagtcgggc
     6781 gtggccgtga accggacga
//
LOCUS       DAORNF010000074         6750 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_8376_length_6750_cov_1.865571, whole genome shotgun sequence.
ACCESSION   DAORNF010000074 DAORNF010000000
VERSION     DAORNF010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 6750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6750
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8376_length_6750_cov_1.865571"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    194..264
                     /estimated_length=71
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    593..639
                     /estimated_length=47
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<640..1398)
                     /locus_tag="PLV68_04355"
     CDS             complement(<640..1398)
                     /locus_tag="PLV68_04355"
                     /inference="COORDINATES: protein motif:HMM:NF017206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA family NAD(P)-binding protein"
                     /protein_id="HRE00506.1"
                     /translation="MTSTTIAAIGCTGPIGGLYVRDLLARGLHVRVLARDADSARAAF
                     PRADVQAGSLLNPADVARALDGVDAALAVTPMGMRNDPEPEIAAGRAIASGAAAAQVP
                     HLIYTSVLGADHRRGVGVLDAKFEIERLLADAGVPCSILRCGSYMEDVFDSRVEMLRK
                     GRWLFPVTATRRFTYTSQHDIPRFVAEALIAGQQVLGGAVNFVSPGTFSVSEVEELLS
                     RAAGWKIRALPKMPVFYAFKALQPVFNYRNSRFSS"
     gene            complement(1395..>1932)
                     /locus_tag="PLV68_04360"
     CDS             complement(1395..>1932)
                     /locus_tag="PLV68_04360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00507.1"
                     /translation="DHVCLGDVGVTWQEQQERMATVTRSVSSTLDGAAIDHLLAGSVG
                     HSPRGDGGALVFHIVTPDGSLLDQDTSGHWTGVVDLLRPDVAIIAAAGRANINGDPIQ
                     GALADYVADHAARCQARTIVLSHHDDWLPGFSIATDVAPIRAALAERCPETVLLELGY
                     VDGTAVFAPPADTKGARP"
     assembly_gap    1933..1942
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1943..2337)
                     /locus_tag="PLV68_04365"
     CDS             complement(<1943..2337)
                     /locus_tag="PLV68_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRE00508.1"
                     /translation="MASHPGTTLDWYGCATFRLRTAGLTIFLDAYIDRVPTAAGAGRR
                     ASDVLECDWILIGHSHFDHLYGAETLLANTSAMLVGSYETVRLMTDAGIDPSRMICVS
                     GGETVDLGHGVTATAYPSLHSCVWSQGAMA"
     assembly_gap    2423..2448
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2449..>3727)
                     /locus_tag="PLV68_04370"
     CDS             complement(<2449..>3727)
                     /locus_tag="PLV68_04370"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HRE00509.1"
                     /translation="IESKGFEGPGPTVSPAMPVMSSQELIEEILLDAARATGLIELRH
                     STEAVELIRGGEDAADDVALRVRSLISGAEEVLTARALVGADGSSSTIREALGLRLEG
                     VKDLLHLCNCYFRADIEAHLGDRRGVLFFVNNEQATGVLQPLDGAGRWLCQINVSPED
                     WSAEMFTKERAREWIRGAAGIDDLDVDVLSIGFWKLNSTVVERFVTGRVVLCGDAAHQ
                     FPPTGGLGVNTGLQGMHNVMWKLAWYVLGRAGWDLVHTYDTERRQPAQDVTSQSLQNS
                     ANVGRINAAAAAGGDSGLNTEPRAATATTSAPSSGRGTHRPLSCPTVPSRQRSRTRTP
                     TTRHPRRPGAVPLTSGSAAPMPTSRPCICSVPTSPCSLARTGRAGRRLRPRSPWTTAH
                     PSTPTDSEPPAWATPERRSRSTVSRLTARSWSAR"
     assembly_gap    3728..3850
                     /estimated_length=123
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4124..4318
                     /estimated_length=195
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4319..4658
                     /locus_tag="PLV68_04375"
     CDS             <4319..4658
                     /locus_tag="PLV68_04375"
                     /inference="COORDINATES: protein motif:HMM:NF028169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TetR-like C-terminal domain-containing protein"
                     /protein_id="HRE00510.1"
                     /translation="RGDLMRILAQTAAFITSPVGAAALAAGLEIGFGDDAGERRALWD
                     GRAIDFAPVFERARERGEIDDRFDDEAVLAMTAGAVYHRVLAMGRHVDDAWISRVVGQ
                     VAVVRPATCL"
     gene            4745..5521
                     /locus_tag="PLV68_04380"
     CDS             4745..5521
                     /locus_tag="PLV68_04380"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRE00511.1"
                     /translation="MPLALITGASSGIGKAFAERLARDGYDLIVVARRRDRLDALAAA
                     LPDVNVRVEVADLSVDAEIDRVAEICLTDALTMLVNNAGLAHYMPLADLPAEKARELI
                     QVKVLAPTMLTRAALPGMIERQTGTIVNVAGMIAFSGPAPSSQMPRRAVYAGTLAHSV
                     AMCQTLHAELEGTGVDVHVVCPGIVATEFHEVQGLDLSSIPRMSADDVVTAALAGIAL
                     GEVVIAPSVEEYALLQQVFDASLAAFGAQATQLAGRYRSA"
     gene            complement(5733..6071)
                     /locus_tag="PLV68_04385"
     CDS             complement(5733..6071)
                     /locus_tag="PLV68_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HRE00512.1"
                     /translation="MTHKEILDELRQPVKDLRERIPAVFEGYGAFSSAVLADGALDAK
                     TKELIALAIAVTKQCDGCVASHARGAARRGATADEVAEALGVAILMNGGPGTVHAPRA
                     FEAFQEFASR"
     assembly_gap    6393..6413
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6423..>6750
                     /locus_tag="PLV68_04390"
     CDS             6423..>6750
                     /locus_tag="PLV68_04390"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRE00513.1"
                     /translation="MTYPILQDKVAIITGAAMGMGAATAQLFAEAGAKVVVADFNEDK
                     GREVVADIEAAGGIAFFQRVDISNSEQVKALVAATVEQYGRLDAAVNNAALTPDDKPL
                     AEFDEAY"
BASE COUNT         1039 a         2115 c         2117 g          986 t
ORIGIN      
        1 aacaacgcgg agcaccacag aaaattccca agaactaacg actcacgaca ctagtccggg
       61 cggtcgacca gcgcggcgag ccgggccgga gcctggatgc agtacgagtc ggtcagcagc
      121 tcgcggacct catcccaatc ggtgccctcg tcgagcagca gaccgacgac gttggtgccc
      181 catccggccc ggannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnngcgtgg gcgacggtcg cctggccgac ccgccaacgc
      301 accccgaccc aggcccgctc ctcgacacac ttgggcaacg actcggaaag cgccgcccgc
      361 aatcgaccga gcatcgccat ggggaggtcg ggtcgttgcg ccatcacccc attctcgttg
      421 ccgggagcgc gcagcggtga cgacgcgcgc gatcgcgagg ggcgccaggt tgcccgccga
      481 cctgaggcgc acgaccagcc tccatccgag gtcggatcag tgggcgaaca gcttcgtcag
      541 gtgttggtcg agggtcgtgg gttcgaacgc cgggaagcga tccgcgatgg tcnnnnnnnn
      601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc gaggagaatc ggctgttgcg
      661 gtagttgaac acgggttgca gtgccttgaa cgcgtagaag acgggcatct tgggcagcgc
      721 ccggatcttc cagcccgcag cgcgactgag cagttcctcg acctcggaga ccgagaaggt
      781 gcctggcgac acgaagttga ccgcgccccc caacacctgc tggccggcga tgagtgcttc
      841 ggcgacgaag cgcgggatgt cgtgctgcga tgtgtaggtg aagcggcggg tcgccgtgac
      901 agggaacagc cagcggcctt tgcggagcat ctcgactcgc gagtcgaaga catcctccat
      961 gtacgagccg cagcgcagga tgctgcacgg cacgcccgcg tcggcgagga gtcgctcgat
     1021 ctcgaacttg gcgtcgagga ctccgacgcc tcggcggtgg tcggccccga ggacggaggt
     1081 gtagatgaga tgcggtacct gggcggcggc cgcgcccgag gcgatcgccc ggccggcggc
     1141 gatctcgggt tccggatcgt tgcgcatccc catcggggtg acggccaagg ccgcatcgac
     1201 accatccagg gcgcgggcga cgtcggccgg attcagcagg cttccggcct gcacgtcggc
     1261 ccggggaaag gccgctcgtg ccgagtctgc gtcgcgggcg agcacccgga cgtggagccc
     1321 cctggccagg aggtcgcgaa cgtagagtcc accgatcggc ccggtgcaac cgatcgcggc
     1381 gatggtggtc gaggtcatgg tcgggctccc ttggtgtccg ccggcggggc gaagacggcc
     1441 gtgccgtcga cgtagcccag ttcgagcagg accgtctcgg ggcagcgctc tgcgagcgcg
     1501 gcccgtatcg gcgcgacgtc ggtggcgatc gagaacccgg gcagccagtc gtcgtggtgc
     1561 gacaacacga tcgtgcgtgc ctggcagcgt gcggcgtgat cggcgacgta gtcggcaaga
     1621 gcgccctgga tggggtcacc gttgatgttc gctcgccccg cggccgcgat gatggcgacg
     1681 tccggacgca gcagatcgac gactccggtc cagtgcccgc tggtgtcctg gtcgagcaac
     1741 gacccgtccg gggtcacgat gtggaacacg agcgccccgc cgtcaccgcg cgggctgtgc
     1801 ccgacagatc ccgcgagcag atggtcgatc gcggcaccgt cgagggtgct cgacaccgat
     1861 cgggtcacgg tcgccatccg ctcctgctgt tcctgccagg tcacaccgac gtcgccgaga
     1921 cacacgtggt ctnnnnnnnn nngccatcgc cccttgcgac cagacgcaac tgtgcaggct
     1981 cgggtaggcg gtggccgtga cgccgtgacc gagatcgacc gtttctccgc cggagacgca
     2041 gatcatccgt gaggggtcga tgcccgcgtc ggtcatcaat cggacggtct cgtagctacc
     2101 gacgagcatg gcgctggtgt tggcgagcag cgtctcggca ccgtagagat ggtcgaagtg
     2161 cgagtgcccg atcaggatcc agtcgcactc gaggacgtca ctcgcgcgac gacccgcccc
     2221 agccgctgtg gggacacggt cgatgtaggc gtcgaggaag atcgtcagtc ccgccgtgcg
     2281 cagccggaac gtcgcgcacc cgtaccagtc gagggtggtg cccggatgac tcgccatcgc
     2341 agcgtcgttt cgggattcgt tgctcatcgg gcgcgagcca tgatcgacga gatcgcctcg
     2401 gccaaccgat cccgtgatgc cgnnnnnnnn nnnnnnnnnn nnnnnnnncc gggcggacca
     2461 agaccgcgcc gtcagcctcg agaccgtaga cctcgagcgt cgctccggtg tcgcccaggc
     2521 cggcggctcc gagtcggtag gtgtcgatgg gtgtgccgtg gtccaaggcg acctcggccg
     2581 cagccgacgc ccagccctgc ccgtccgggc cagcgagcag ggtgaggttg ggaccgaaca
     2641 gatgcagggt cgagaggtgg gcatcggcgc ggccgagcca gacgtgaggg gcacggcacc
     2701 cgggcgtcgc ggatggcgcg tagtcggtgt acgggtcctc gaccgttggc ggctcggtac
     2761 cgtcggacac gacagcggcc gatgtgtacc gcgccccgaa ctcggtgccg aggtggttgc
     2821 cgtagcggcg cgaggctcgg tgttcagccc gctgtcccct cctgccgcgg cagcggcgtt
     2881 gatccgtccg acgttggccg agttctgcaa cgactggctg gtgacgtcct gcgcgggttg
     2941 gcgtcgctcg gtgtcatagg tgtgcaccag atcccagccg gcacggccga ggacgtacca
     3001 cgcgagcttc cacatcacgt tgtgcatgcc ctgcagtccg gtgttgacac ccaggccgcc
     3061 ggtgggaggg aactgatgcg cagcgtcgcc acacaacacg acacgcccgg tgacgaaccg
     3121 ctcgacgacg gtggagttga gcttccagaa cccgatcgac aacacgtcga cgtcgagatc
     3181 gtcgatgccg gccgcgccgc ggatccactc ccgcgcgcgc tccttggtga acatctcggc
     3241 ggaccagtcc tcgggagaca cgttgatctg gcacagccag cggcccgcac cgtcgagcgg
     3301 ctgcaggacg ccggtcgcct gctcgttgtt gacgaagaac agcacgccgc gccggtcacc
     3361 gaggtgcgct tcaatgtcgg cgcggaagta gcagttgcac aggtgcagca ggtccttgac
     3421 gccctcgagg cgcaggccga gcgcttcgcg gatggtgctc gacgatccgt ccgcgccaac
     3481 gagcgcccgt gcggtgagca cctcctcggc gccggagatg agcgatcgaa cccgcaacgc
     3541 gacgtcgtcg gcagcgtcct caccaccgcg aatcaactcg accgcttcgg tggagtgccg
     3601 cagttcgatg agcccggtgg cgcgtgctgc gtcgaggagg atctcttcga tcagctcttg
     3661 cgacgacatc accggcatcg ccgggctgac cgtgggaccg ggcccctcga agcccttcga
     3721 ctcgatcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn ccatcgtgcg cgtgttgaag ttcctggtct tgggatgcca cgacgtgccg
     3901 acgtgtcgtt cgatcagcac gctccggatg ccgaagcggg ccagctccaa tgctgcaccg
     3961 agacccaccg gaccgccgcc gacgatgatc accctctgca cccgacatgc gaacccccac
     4021 tcctcgaacc gctagcgaga caaatgtatc attacgtcat gcccgctggg aacacgccac
     4081 catcgccccc cgatcggccc tccaatcggc ccggcggccg cacnnnnnnn nnnnnnnnnn
     4141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncc
     4321 gcggcgacct gatgcggatc ctggcccaga ccgctgcttt catcacctca ccagtcgggg
     4381 ccgccgcgct cgcggcgggc ctggagatcg ggttcgggga cgacgccggc gaacggcgag
     4441 cgctgtggga tggccgggcg atcgacttcg ccccggtgtt cgaacgggcc cgcgaacgcg
     4501 gcgagatcga cgatcggttc gacgacgagg ccgtgctggc gatgacggcc ggcgccgtct
     4561 accaccgggt gttggcgatg gggcgccacg tcgacgacgc gtggatcagc cgggtcgtcg
     4621 gtcaggtcgc ggtagtccgg ccggcgacct gtctgtagcg gccatcgagc gtcagtcgca
     4681 ctgacgttcg cggcgctcga ttgctcggtg gacgccagcg caactgtggt gcaggatgcg
     4741 cagcatgccg cttgcgctca tcactggtgc ttcgtccgga atcggcaaag cgttcgccga
     4801 acggctcgca cgcgacggct acgacctgat cgtcgtcgct cgacggcgtg accggctcga
     4861 cgccctcgct gccgcgttgc ccgacgtgaa cgtacgggtg gaggtggccg atctgtcggt
     4921 ggacgccgag atcgatcggg tcgcggaaat ctgcctgacg gatgcgttga ccatgctggt
     4981 caacaacgcc ggcctggcgc actacatgcc actcgccgac ctccccgccg agaaggctcg
     5041 cgagctgatc caggtcaagg ttctcgcccc gacgatgctc acacgcgctg cgctgcccgg
     5101 gatgatcgag cgtcagacag gaacgatcgt gaacgtggca gggatgatcg ccttttccgg
     5161 gccggccccg tcatcgcaga tgccgcgccg ggcggtgtac gccgggaccc tcgcccactc
     5221 ggtcgccatg tgccagacct tgcacgccga gttggagggc acaggtgtcg acgtccacgt
     5281 cgtgtgtccc gggatcgtgg ccaccgagtt ccacgaggtc cagggattgg acctgtcatc
     5341 aataccccgg atgtcagccg atgacgtcgt cacagcggca ctcgccggga ttgcgctcgg
     5401 cgaggtcgtc atcgcaccga gcgtcgagga gtacgccctt ctccagcagg tgttcgacgc
     5461 cagcctcgct gccttcgggg cgcaggcgac gcaactggca ggtcgatacc gatcggcgtg
     5521 aacagaccac ggtcgtcttt gggcgagttc gtcgacgcgt ccctgagcgc gtcaccgatc
     5581 cggatactgc cgaacgttcg atggctccgg cttgatggcg tcggtgatga catccaccgc
     5641 ttccttgacg tgcaatgtcg gtgtgggtcg ggccgaggac cgccagatcg tgcacgccca
     5701 cagcatcgac agcagtgggt cagccaccag ggtcatcgac tggcgaactc ctggaacgcc
     5761 tcgaaagcgc ggggtgcgtg cacggtgccc ggtccgccgt tcatcaagat cgcaacaccg
     5821 agtgcctcag cgacctcgtc ggctgtggca ccgcgccggg ccgcgccacg cgcatgagag
     5881 gccacgcagc cgtcgcattg cttggtcacc gcgatcgcga gcgcaatcag ctccttggtc
     5941 ttggcgtcca gagcgccgtc agcaaggacc gcgctgctga aggcgccata gccctcgaac
     6001 actgcaggga tgcgctcgcg caggtccttc accggttggc ggagttcgtc gaggatctct
     6061 ttgtgggtca tacgattcct tcgagaacgg ttcgtggtcg gcgcgtttgc caccgccatc
     6121 ctcgccccgt tcacggggcg ggtcaacaga tcattgacgt ggtgctcgta cgcctgtgtc
     6181 gagcgcatcc gtcggcgacc cggacctcga tgaactcgac cccggacaat tctggtgggc
     6241 gtatcatcgc ggcatgtgtg aacgagatcg cgatgaccct cgagttcgaa gtgtccgcca
     6301 tccgtaccgg cgactgacac gagcgccggc gccagccatc cgtcgggaag ccgccacccc
     6361 gcacttgcga cacgatgacc gcagacctca cgnnnnnnnn nnnnnnnnnn nnnaccacga
     6421 ccatgacata tcccatactc caggacaagg tggccatcat caccggcgcc gcgatgggca
     6481 tgggcgccgc caccgcccaa ctcttcgccg aagccggcgc caaggtcgtc gtcgccgact
     6541 tcaacgagga caagggcaga gaagtcgtcg ccgacatcga agccgccggc ggcatcgcgt
     6601 tcttccaacg cgtcgacatc tccaactccg aacaggtcaa ggccctcgtc gccgccacag
     6661 tcgagcagta cggccgcctc gacgccgccg tcaacaacgc cgcgctcacc cccgacgaca
     6721 aaccactcgc cgagttcgac gaggcctact
//
LOCUS       DAORNF010000075         6726 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_8421_length_6726_cov_1.543996, whole genome shotgun sequence.
ACCESSION   DAORNF010000075 DAORNF010000000
VERSION     DAORNF010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 6726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6726
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8421_length_6726_cov_1.543996"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            175..>757
                     /locus_tag="PLV68_04395"
     CDS             175..>757
                     /locus_tag="PLV68_04395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00514.1"
                     /translation="MKSTLDPYWYAGAPNVAFAFSSDETPGAFTYKELNASLVLTWDK
                     LPYIPDSGQSPAANPYLFHDHLNGISLSIPPLTDPDGETLYYRFVLCATNTWVGCAGS
                     GMIRDDSGWLSSPSYSKWLSVGFPASWYNQQLYWGVQVSNLTSSGYVMQSWWMRPWKL
                     YNNSAASPSLISPVDGFTWVPSAPPTLQFSTPSD"
     assembly_gap    758..767
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1375..1518)
                     /locus_tag="PLV68_04400"
     CDS             complement(1375..1518)
                     /locus_tag="PLV68_04400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00515.1"
                     /translation="MLFVAGVRPSGSPAAMVAMVPDQLTSNAVAPLTFTWVRSSPFSD
                     AAK"
     gene            1572..>2245
                     /locus_tag="PLV68_04405"
     CDS             1572..>2245
                     /locus_tag="PLV68_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00516.1"
                     /translation="MSLTAGVPVPITVIYRNPGGVGQFNLYQSKSTGTNFARIPSSWL
                     TTAARVLPRGWVFNHSEVNGALYSSARADADSIVVTYTDGSTGTFTRNASGVFVDPEG
                     DGAQAVLSAGVITVTDEAGYAHVFNNDGTLKTVTAPIDVRVNATATPTWTSWTPSGLT
                     QAASRLTAMTDPASGRQVTYHYQGVSGSSCPSVPGGFVAPFEGMLCKVGYPDGTVTNL
                     YYTSAAG"
     assembly_gap    2246..2489
                     /estimated_length=244
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2490..>2703
                     /locus_tag="PLV68_04410"
     CDS             <2490..>2703
                     /locus_tag="PLV68_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00517.1"
                     /translation="SRQSFAIAYTSTVETRVMIAGLDNPSDPNDFDRKVNFDATARVT
                     YDYQAANATSSQTMVTETRWHPSIDRA"
     assembly_gap    2704..2824
                     /estimated_length=121
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2825..>3213
                     /locus_tag="PLV68_04415"
     CDS             <2825..>3213
                     /locus_tag="PLV68_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="PA14 domain-containing protein"
                     /protein_id="HRE00518.1"
                     /translation="TNPPVGRTSTEYDTVLNANGTSSPMTGLGVTVWSNTTFSGQPSN
                     VATGIGPNYTTLNNTWATGGPPEAVDNAGNPLSTHSLRLTGEIVFPATGTWTFWAGYD
                     DITRLYIDNQLVVNAGCCSTGSGTFNV"
     assembly_gap    3214..3223
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3224..>4245
                     /locus_tag="PLV68_04420"
     CDS             <3224..>4245
                     /locus_tag="PLV68_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00519.1"
                     /translation="GDLTKRITIEHVNTGGPGVLNVQWAGPGVAQQYLPTSALRPAYG
                     LVTRTTVHDSNPASPPQITHTEYTATGIDAALGMATRVTVDPAGARLTTTTGYETSGY
                     RRRTSRTLPGGSATTYDYYAPSAGAAVNVPCTAWDDTTINQGGMLRKTTEPTAATGAA
                     IISEAVYDSMGRQVATRVGTRSGGVDTWSSSWTCVTYDGRGRITQTVHAARGLLPGRT
                     VTNSYSIGADPRVNSISDNVGTITTTTDLLGRTVTSTDVWGQTVANAYDQTTGRLTST
                     TDAAGVDGYTYTRDGRLVQQSFNGQPIATPSYLAPGNANEYQVDSVTYPGGGGNAGNA
                     TNGTFT"
     assembly_gap    4246..4276
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4349..>5020
                     /locus_tag="PLV68_04425"
     CDS             4349..>5020
                     /locus_tag="PLV68_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding domain-containing protein"
                     /protein_id="HRE00520.1"
                     /translation="MVDESVDGTDPYAAGPNYRHDSVGRLASARVSSNVYEYGYDNTP
                     NCGANPTAGANTNRTDTRVNGGVATTYCYDHTDRVTSYGTAPGTYAQMTTPGAPGYLW
                     RLGETSGTTATATYGGGGANGTYSATTVQTAGFEGTFDGWAKWPGSGMTVTNPSNPAV
                     AHTGSGYLAATAATAGDSVYRDTTYSVQRGATYRMTAWIKSASGSAISGTLTLWGIGG
                     SRTSAA"
     assembly_gap    5021..5051
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5052..>5360
                     /locus_tag="PLV68_04430"
     CDS             <5052..>5360
                     /locus_tag="PLV68_04430"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00521.1"
                     /translation="QKITFDYTPTVATHTAFREQFYVTTTGQSLLIDDVSITANVIEL
                     ADAGAPTGSVDPSAKFVGGNVAIPVAALTGSGKTFSLWFKTTSTAGGVLLSKNNGAVG
                     S"
     assembly_gap    5361..5386
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5387..6627
                     /locus_tag="PLV68_04435"
     CDS             <5387..6627
                     /locus_tag="PLV68_04435"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HRE00522.1"
                     /translation="PALASPHAGMSANITTRTAVNDGLWHHAIFTVSATTQTLYVDGT
                     KIGAVSGTVNDSWGPTHAYIGTGYTNTWPATNNTWMPFSGSIDEVAVYNTVLTDSQVA
                     TITTPAPTTNVSTPVYDDYGNTVADAGQELTYDSTGRNTGMYTPSVASPTTSVTYVRD
                     ALDQIVARTATVSGVATTDHYSGRMVLDVTNTVIIEYTIGLPGGVAVTKRSGGDVWSY
                     PNLHGDVQATADAAGIKIGATYRYDPFGQPIGTIPDNHAGEADNAWVGQHRKLYEHQT
                     GLTPLIQMGARVYNPATGRFLQVDPIEGGTANPYEYPSDPINQYDLTGLASACPGKYK
                     YGGSVLGDYSSKICIQSIHLQLLTQPELNRQSKLDSRSSPVGSAWAVDSSVGSARNWC
                     KNTVSVQGEYWTRLDDLYLR"
BASE COUNT         1198 a         2051 c         1969 g         1035 t
ORIGIN      
        1 gccagtctga acgtgtcgtg gtacgccggg acgtcgaact cacaggcgat cgcgattcgg
       61 catgcgacga gtttcgactt ctgcaccgtg cacgtgggtg gcaactgttc gaacggctat
      121 tccccgttcg cggcaccgat tcagtacatc acgacaggat cagcatcgtt cgacgtgaag
      181 tcgacgctcg atccgtattg gtacgcgggt gcaccgaacg tggcattcgc gttctcgtcg
      241 gatgagacgc cgggcgcgtt cacctataag gagttgaacg cgtcgttggt gttgacatgg
      301 gacaagctgc cgtacatccc ggacagcggc cagtcgccag cggcgaatcc gtacctgttc
      361 cacgatcacc tcaacggcat cagtttgagc atcccgccgt tgaccgatcc agatggcgag
      421 acgctgtact accggttcgt gctgtgcgcg acgaacacct gggtgggttg tgccgggtct
      481 gggatgatcc gggacgactc ggggtggttg tcgtcgccgt cgtacagcaa gtggctcagc
      541 gtcggatttc cggcgagttg gtacaaccag cagttgtatt ggggtgtgca ggtctcaaac
      601 ctgacatcga gtggctacgt gatgcaatcg tggtggatgc ggccgtggaa gttgtacaac
      661 aactcggcgg cctcgccttc gttgatctca ccggtcgacg ggttcacgtg ggtgccgtcg
      721 gcgccaccga cgctgcagtt ctccacacca tcggaccnnn nnnnnnngcg attgggtgcg
      781 ctatcgggcc gtgatccgca cgaagggaac gggcggcacc gccttcgtca ccgattgggg
      841 tgcggcggtt gcgccgggta cttcttcgtc gtccatcacg ctgccgtcga cagcaccgct
      901 ggtggcgggg gtgacctata cgtggacgat cgagcacatc gacgcggtgc agtacttcca
      961 ctggtactac taccgcggcc aaccgcagca gcccaacgcg gtgacacggg cagcgagctt
     1021 cgatgatcgt cttggtgcgt cgggaccgtc gccgatgcag tcgctcggcc cggtcggtgt
     1081 caacttggcg accggcaacg tcacgacgtc ggtggtgaca ccgcagatcg ccacgttggg
     1141 cggggcgttg ggcgcttcgt tcacctacaa ctcgcgtccg aacgacaccg ggctgagtgc
     1201 ccgctacgtc aacgacgcca atggcaacgg tgtcgcggac ggcggcgaac aggtgtcgcc
     1261 atcgacggtg cgcgagctgt cgttcgactg gtcctcgccc gcggcaattc caggcacgac
     1321 gaacctgttg gccacgtggt ccggctatct gaccgtgccg gtatcgggca cctactactt
     1381 cgcggcgtcg ctgaacggcg acgaacgcac ccaggtgaag gtcaacggag ccacggcgtt
     1441 ggaggtcaac tggtccggca ccatggcgac catcgccgct ggcgacccgc tggggcgcac
     1501 tcctgcgacg aacagcaaga actacttcga cacctccgag ttccccaacg tcggcgccgg
     1561 gtcgtcaggg tgtgtcgctg accgctggag tgccggtgcc gatcacggtg atctaccgca
     1621 accctggcgg tgtcggccag ttcaacctgt atcagtcgaa gtccacgggc acgaacttcg
     1681 cgcgtatccc gtcgtcgtgg ttgaccaccg cagcgcgggt gctaccccgc ggttgggtgt
     1741 tcaaccactc cgaagtgaac ggcgccctct acagctcggc ccgcgccgat gccgactcga
     1801 tcgtggtcac ctacaccgat ggttcgacgg ggacgttcac gcgcaacgcc agcggcgtgt
     1861 tcgtcgaccc ggagggagac ggcgcgcaag cggtcctcag tgctggggtg atcacggtga
     1921 ccgacgaagc tgggtatgcg cacgtgttca acaacgacgg caccctcaag accgtgacgg
     1981 ccccgatcga cgtgcgggtc aacgcgaccg cgacaccgac gtggacgtca tggacgccga
     2041 gcggactgac ccaggccgcg tcgcgtctga ccgcgatgac cgacccggcg tcgggccgtc
     2101 aggtgaccta ccactaccag ggcgtgtccg ggtcgtcgtg cccgagcgtg ccgggtggtt
     2161 tcgtcgcccc gttcgagggc atgttgtgca aggtcggcta tccggacggc accgtcacca
     2221 acttgtacta cacgtctgct gcgggnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc cagccgccag tcgttcgcga tcgcgtacac
     2521 gtcgaccgtg gagacgcggg tgatgatcgc cgggctcgac aacccgtcag accccaacga
     2581 cttcgaccgc aaggtcaact tcgacgccac cgccagggtg acctacgact accaggcagc
     2641 caacgccacg tcgtcgcaaa cgatggtgac cgagacccgc tggcatccca gcatcgatcg
     2701 agcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnngcacga acccaccggt cgggcggacc tccacggagt acgacacggt gctcaacgcc
     2881 aacggcacct cgtccccgat gaccggcctc ggtgtgaccg tgtggtcgaa caccaccttc
     2941 agcggtcaac cgtccaacgt cgccaccggg atcggcccca actacaccac gctcaacaac
     3001 acctgggcga ccggtggccc accggaagcg gtcgacaacg ctggcaaccc gctgtcgacc
     3061 cactcgctgc ggctgaccgg cgagatcgtg tttccggcga ccggaacgtg gacgttctgg
     3121 gccggctacg acgacatcac ccggttgtac atcgacaacc agctcgtggt caacgccgga
     3181 tgctgtagca ccggctcggg cacgttcaac gtgnnnnnnn nnncggagat ctgaccaagc
     3241 ggatcacgat cgagcacgtc aacaccggcg gccccggcgt gttgaacgtg cagtgggctg
     3301 gccccggcgt cgcccagcag tatctgccga ccagtgcgct gcggccggcg tacgggttgg
     3361 tcacccgcac cacggtgcac gattcgaacc ctgcatcgcc accgcagatc acccacaccg
     3421 aatacaccgc gaccggtatc gacgcggcgc tcggcatggc cacccgggtc acggtcgacc
     3481 cggcgggggc gaggttgacg accaccaccg ggtatgagac ctcgggatat cggcggcgga
     3541 cgtcgcggac gttgccgggc ggcagcgcca cgacgtacga ctactacgcg ccctccgcgg
     3601 gcgcggcggt gaacgtgccg tgcaccgcat gggacgacac cacgatcaac caaggcggca
     3661 tgctccgcaa gaccacggaa ccgaccgccg cgaccggcgc agcgatcatc tccgaagctg
     3721 tctacgacag catggggcgc caagtcgcaa cacgagtcgg tacgcgcagc ggcggcgtcg
     3781 acacgtggtc gtcgagttgg acctgtgtca cgtacgacgg acgcggccgg atcacccaaa
     3841 ccgttcacgc cgcacgcgga ttgctgcccg gccggaccgt caccaacagc tacagcatcg
     3901 gcgctgaccc gcgggtcaac tcgatcagcg acaacgtcgg caccatcacc accacgaccg
     3961 acctgctcgg tcgcaccgtc accagcaccg acgtttgggg ccagaccgtc gccaacgcct
     4021 acgaccaaac caccgggcgg ctcacctcga ccaccgatgc tgccggggtc gacggctaca
     4081 cctacacccg agacgggcga ctcgtacagc agagcttcaa cggtcaaccc atcgccacac
     4141 cgagctacct cgctccgggc aacgcgaacg agtaccaggt cgattcggtc acgtatccgg
     4201 gtggtggcgg caacgctggc aacgcgacca acgggacatt cacatnnnnn nnnnnnnnnn
     4261 nnnnnnnnnn nnnnnngtgt cttggcggcg taacagcccg acgacgacga tcacgtcggc
     4321 cagtgtgacg cgttcccagt ctggccgtgt ggttgacgaa tcggtcgatg gcaccgatcc
     4381 gtacgcggcg ggaccgaact accggcatga cagcgtgggc cgtctcgcga gtgcgcgggt
     4441 ttcgagcaac gtgtacgagt acgggtatga caacacgcca aactgcggtg ccaatccgac
     4501 ggcaggtgcg aacacgaacc gcaccgacac ccgtgtcaac gggggcgtcg cgaccaccta
     4561 ttgctacgac cacaccgatc gcgttaccag ctatggcacc gcccccggca cctacgcgca
     4621 gatgaccact ccgggtgcac ctggctacct ctggcggctg ggcgagacgt ctggcaccac
     4681 cgctaccgcg acgtacggcg gtgggggtgc gaacggcacg tacagcgcaa ccacagtgca
     4741 gacggcaggg ttcgagggca cgttcgacgg gtgggcgaag tggccaggca gtgggatgac
     4801 cgttacgaac ccgtccaacc cggcggttgc ccacaccggc agcggatacc tggcggcgac
     4861 cgctgcgacc gcgggcgaca gcgtctatcg cgacaccacc tacagcgtgc agcgtggtgc
     4921 gacctaccgg atgaccgcat ggatcaagtc ggcgtccggg agtgcgattt cgggcacgct
     4981 gacgctgtgg ggcatcggcg gcagcaggac cagcgcggcg nnnnnnnnnn nnnnnnnnnn
     5041 nnnnnnnnnn ngcagaagat caccttcgac tacaccccga cggtggcgac tcacacggcg
     5101 ttccgtgagc agttctatgt caccaccact ggccagagcc tgttgatcga tgacgtcagc
     5161 atcactgcga acgtgatcga gctcgccgac gctggcgcgc ccaccgggtc tgttgatccc
     5221 agcgccaagt tcgtcggcgg caacgtggcg attccggtgg ctgcgttgac ggggagcggt
     5281 aagacgttct cgttgtggtt caagaccacg agtactgcgg gtggggtgtt gttgtcgaag
     5341 aacaacggtg ctgtcgggtc nnnnnnnnnn nnnnnnnnnn nnnnnncgcc ggcgttggcc
     5401 agtccccacg cgggcatgag cgcgaacatc accacgcgga ccgcggtcaa cgacgggttg
     5461 tggcaccatg cgatcttcac cgtgtcggcc acgacgcaga cgctgtatgt cgacggcacc
     5521 aagatcgggg ccgtgtctgg cacggtgaac gacagttggg ggccgacgca cgcctacatc
     5581 ggtaccgggt atacgaacac gtggccggcg acgaacaaca cgtggatgcc gttcagcggc
     5641 agcatcgacg aggttgccgt gtacaacacg gtgttgaccg atagccaggt cgccaccatc
     5701 acgacgcccg ctccgacaac gaacgtgagc acgccggtgt atgacgacta cggcaacacc
     5761 gtcgctgatg ctggtcagga gttgacctac gactcgaccg gccgtaacac cggcatgtac
     5821 acgccctcgg tggcgtcccc gacgaccagc gtcacctatg tccgtgacgc gctcgaccag
     5881 atcgttgctc gcaccgccac tgtctccggg gtggcgacga ccgatcatta ctcgggtcgg
     5941 atggtgctcg acgtcacgaa cacggtgatc atcgagtaca cgatcggtct ccccggcggg
     6001 gtagccgtca cgaaacggtc cggtggggac gtgtggtcgt atccgaacct gcacggtgac
     6061 gtgcaagcca ccgccgacgc agccgggatc aagatcggcg ctacctaccg gtacgacccg
     6121 ttcggtcaac cgatcggcac gatccccgac aaccacgcgg gggaagccga caacgcctgg
     6181 gtcggccaac accggaaact gtacgaacac caaaccgggc tcaccccact catccaaatg
     6241 ggagcccgcg tctacaaccc cgctaccggt cgcttccttc aagtagaccc catcgaaggc
     6301 ggcaccgcca acccctacga atatcccagc gaccccatca accaatacga cctcaccggg
     6361 cttgcttcag cgtgtcctgg taaatacaaa tatggtggaa gtgttttggg tgactattca
     6421 tcaaagatat gtattcagtc aatacactta caacttctca ctcagcccga actcaatcgg
     6481 cagtctaaac tggattcgcg ttctagtcct gtcggcagtg cctgggccgt ggattcttca
     6541 gtcggaagtg cgaggaactg gtgcaagaac acagtatcag tccaaggtga gtactggact
     6601 cggctggacg acctttacct tcggtaaata caactacgcc acggggtcga agacaggtgc
     6661 catttccatg tcatttaacc ggcgcttcgg atctgacggt gggttgacgg tcgggtgaga
     6721 tcccgg
//
LOCUS       DAORNF010000076         6706 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_8454_length_6706_cov_1.744850, whole genome shotgun sequence.
ACCESSION   DAORNF010000076 DAORNF010000000
VERSION     DAORNF010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 6706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6706
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8454_length_6706_cov_1.744850"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="PLV68_04440"
     CDS             complement(<1..323)
                     /locus_tag="PLV68_04440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00523.1"
                     /translation="MQLTIAQPDRRDVEAQRVEPPPPLFLDETPPRTLDGAGDHDAEA
                     AAGLRGEFHQTGIDAVVAVDSNCIRREDQRWFPRVAAVMPGWTGEITSRAKIASASDT
                     AWSGPA"
     gene            336..1070
                     /locus_tag="PLV68_04445"
     CDS             336..1070
                     /locus_tag="PLV68_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00524.1"
                     /translation="MLAEAAQPAIPPAEPGDLSLYADPVWSDFTAAHAAPDAVPSLPA
                     DDRDHAPTVVSIAGVTRPATSLDARHAASGDGSAESSDAGHAASGIGPTDPVDGQQAA
                     VGTWLAEAHHGSHAADGDGAPDRCRAGTAEGRMGGGSDASDERPERTGWVEPEWPLMV
                     RVLAGNVDVVDRGGRAVAFERAKALELVVWLSQHRERSTRSGARTALWDLDVRDLGQR
                     GLGCPPGDGAVGADHWRARVGGPHDQ"
     gene            1075..>1366
                     /locus_tag="PLV68_04450"
     CDS             1075..>1366
                     /locus_tag="PLV68_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00525.1"
                     /translation="MPLHPAVVTDADLLASRLAAAQRLARDEAIAVLRPGLEPVQDLP
                     FAGTSYLWPDTEGITSQLILLVTSSAATLGEWYLAADDIDGVFWATGRSLSVL"
     assembly_gap    1367..1480
                     /estimated_length=114
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1695..2279
                     /locus_tag="PLV68_04455"
     CDS             1695..2279
                     /locus_tag="PLV68_04455"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HRE00526.1"
                     /translation="MAPLLTRQSIVKVTDQAPDDDQIEAILRAAVTVPDHGAVRPWRL
                     VVVSGDARHAFGNALSAAGQQARVDMPDQVLERLRSKAFVAPALIAVVARIVPESGID
                     VWEQVASAACAGYAITLAAHQLGLGAVWKSSPFRDGAELRRVLDMGEHDEFLGWVNLG
                     GIPQARPASKRPEIDLTEIARALSPEGVPTPYQR"
     gene            complement(2316..3386)
                     /gene="disA"
                     /locus_tag="PLV68_04460"
     CDS             complement(2316..3386)
                     /gene="disA"
                     /locus_tag="PLV68_04460"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:NF010009.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning diadenylate cyclase DisA"
                     /protein_id="HRE00527.1"
                     /translation="MPMQRRPDELLNALARVAPGTPLRDGLERIVRAKMGALLVLSDD
                     PAVLEICSGGFLLDAPFSPQRLSELAKMDGAIILNADLSRIARANVHLLPDASVPTAE
                     TGTRHRTAERVARSLSAPVVSVSEEMSVINLYAGANKHQLQEVGRLLDRANQALQTLE
                     RYKVRLDDAMSNLTAQEVEDVVSLRDVASVVQRGEMVHRIAEEIETMIVELGVDARLL
                     RLQLDELYGEIDDELDLVIRDYTPVSSAVEDVHTFMARLDDDGVLDLRRTAVALDPVG
                     GADDLDGSVSSRGMRLLARVPRVSDELAAAVTDHFGELTKLQRATVADLMEVPGMSPA
                     QAQAIKDTLERVVESTIMDQYN"
     gene            complement(3421..>4551)
                     /gene="radA"
                     /locus_tag="PLV68_04465"
     CDS             complement(3421..>4551)
                     /gene="radA"
                     /locus_tag="PLV68_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HRE00528.1"
                     /translation="GSVTLLGGEPGVGKSTLLLQLLASWVGRTLYVSAEESAQQVRLR
                     GERLAAIRPELWLLPETSLPNIVAALGDLQPQLVVIDSIQTIADPELSSAPGSVSQVR
                     GCANRLVQEAKRRGITMVLVGHVTKEGGLAGPRVLEHVVDTVLSFDGERHHALRLLRA
                     AKHRFGPTDELGLFEMGEYGLAPVPDPSQLFLADRRTGVPGSAVVPVVEGHRPLLVEV
                     QALTNIVPGGGTLRRSAHGIDANRLAMLIAVIERRARLPVAAHEVFVSVVGGVKLTEP
                     GIDLGLCLAIASAMIGQPLPANLVACGEVGLAGELRQVSQTHRRLGEASRLGFATAIV
                     PASAPEHSGPIRVLRASTLPEALRLAGLSSGDRPGGTLRTMP"
     assembly_gap    4552..4684
                     /estimated_length=133
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4685..4830)
                     /locus_tag="PLV68_04470"
     CDS             complement(<4685..4830)
                     /locus_tag="PLV68_04470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00529.1"
                     /translation="MAKSRVVHRCSECGAAHPKWSGRCAACGAWNSLTEEVEAADEPV
                     SLAAG"
     gene            5188..>5466
                     /locus_tag="PLV68_04475"
     CDS             5188..>5466
                     /locus_tag="PLV68_04475"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRE00530.1"
                     /translation="MTGAGSGIGLALAEALARAGCSVVLADVQQDALDAAAATVGAYG
                     VETLCVRTDVSKLDQVEALAQATLDRFGAVHVVCNNAGVAGAGDPWVGP"
     assembly_gap    5467..5500
                     /estimated_length=34
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5501..6130
                     /locus_tag="PLV68_04480"
     CDS             <5501..6130
                     /locus_tag="PLV68_04480"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRE00531.1"
                     /translation="GVVHGVRTFLPHIVASGGGHIVNTASIAGLFPGFAAPYDASKHA
                     VVAMTESLFNSLQMSPVPVGVSCLCPGWVRTGILDADRNWPPELGARPEVDPISAISR
                     EYVRRAIDEGTQAESRRAKAAEAQAELVADLVLDAVRANRFWVFPNPDFLEIAVARFH
                     TIGEGLDPSPPEHVPGMPPRSTMMAEVLEAVMATMAPTPAPEPDSDPPT"
     gene            complement(6219..>6706)
                     /locus_tag="PLV68_04485"
     CDS             complement(6219..>6706)
                     /locus_tag="PLV68_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HRE00532.1"
                     /translation="KVGAATEVELKEKKHRIEDAVSTTKAAIEEGVVPGGGVALLRAQ
                     AKVLEAAAALSGDEATGARLVAKSLEAPLKQIAENAGLEGGVVVEKVKGLTGNEGLNA
                     ATGEYEDLVKLGVIDAAKVTRSALQNAASIAALFLTTEAVVADKPEDAPAMPGGGMED
                     F"
BASE COUNT          921 a         2213 c         2266 g         1025 t
ORIGIN      
        1 gcggggccgc tccatgccgt gtcgctggcc gaggcgatct tcgcccgcga cgtgatctcg
       61 cccgtccatc ccggcatcac ggcggcgacg cgtgggaacc agcgctggtc ttcgcgacgg
      121 atgcaattgc tgtcgacagc gaccacagcg tcgatgccgg tctggtggaa ctcaccgcgc
      181 agcccggccg cggcctcggc gtcgtgatcg cccgccccgt cgagggtgcg cggtggcgtc
      241 tcgtccaaga acagcggcgg tggcggctcg acccgttggg cctcgacgtc gcgccggtcg
      301 ggctgagcga tcgtgagctg caacgggtcg cgaccttgct cgccgaagca gcgcaacctg
      361 ccatcccgcc cgccgagccc ggtgatctgt cgctgtacgc cgacccggta tggagcgact
      421 tcaccgccgc ccatgccgcc cctgatgcgg tccccagcct ccctgccgac gatcgagatc
      481 acgcaccgac cgtggtgagc atcgctggcg tcacccgccc ggcgacctcc ctcgatgccc
      541 gacatgcagc gagcggcgat ggatcggccg agtcgtccga tgccgggcat gccgccagcg
      601 ggatcggccc gactgacccc gtcgatggcc agcaggcggc ggtcggcacg tggttggccg
      661 aggcgcacca cggcagccat gctgccgacg gcgatggggc acccgatcgg tgccgtgccg
      721 gcacggcgga gggccggatg ggcggaggct ccgacgcctc ggacgagcgc ccggagcgga
      781 cggggtgggt cgaaccggag tggccgttga tggtgcgggt tcttgcaggc aacgtcgatg
      841 tggtcgaccg cggtggtcgc gccgtggcgt tcgagcgggc caaggcgttg gaactggtgg
      901 tgtggctcag ccagcatcgc gagcgctcga cccgctcggg ggcgcgcacc gcattatggg
      961 acctcgacgt ccgcgacctt ggccaacgtg gtctcggatg cccgccgggc gatggcgcgg
     1021 ttggtgccga ccactggcgg gcaagagtgg gtgggccgca cgatcaatga tcagttgccg
     1081 ctgcacccgg cggtcgtcac cgacgccgac ctcctggcgt cacggctcgc ggccgcccag
     1141 cgcctggcgc gcgacgaggc gatcgccgtg ctccgtcccg gtctcgaacc cgtgcaggat
     1201 ctccccttcg ccggcacgtc gtacctgtgg cccgacaccg aaggcatcac gtcgcagctg
     1261 atcctgctgg tgacgtcgtc ggcggcgacg ctgggggagt ggtacctggc cgctgacgac
     1321 atcgacggag tgttctgggc gaccggccgg tcgctatcgg tgctggnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gcggtggcga cccgtcgccc
     1501 aagctggtcg cgctgcgccg cgagctcctt gctggcgcga tgttgacggc ctgacgatgc
     1561 ggcgcgcctg aacgtgcgat gtcgcagcgc ggcgacccga tgcgcagcaa cgatccagcc
     1621 ggtatcgtta ggcattccta acggacgaag aagaagagga catcaccgtg ctacttgcgg
     1681 atcatgaagt cgccttggcc ccgttgctca cccgccaaag catcgtcaag gtcaccgatc
     1741 aggcccccga tgacgaccag atcgaagcga tcttgcgcgc tgcggtgacc gtccccgatc
     1801 acggtgcggt gcggccctgg cggttggtgg tcgtcagcgg cgacgcgcgc catgcgttcg
     1861 gcaacgcgct cagcgccgcg ggtcagcagg cgcgtgttga tatgccggat caagtgctcg
     1921 aacgcttgcg gagcaaggcg ttcgtggccc cagcgctgat cgccgtcgtc gctcgcatcg
     1981 tgccagagtc gggcatcgac gtgtgggagc aggtcgcctc ggctgcctgt gccggatacg
     2041 cgatcaccct ggccgcgcac cagttgggcc tcggcgcggt gtggaagagc agcccgttcc
     2101 gtgacggcgc cgaactgcgg cgggttctcg acatggggga gcacgatgag ttcctcggct
     2161 gggtcaacct cggtggcatc ccccaggctc ggccggccag caagcgccct gagatcgacc
     2221 tgaccgagat cgcccgtgcg ctgagccccg aaggagtgcc caccccgtat cagcgctgac
     2281 gctcacgttg tgggcgactg gttgtggtgg ctgcgtcagt tgtactggtc catgatcgtg
     2341 gactcgacca cccgctcgag ggtgtccttg atggcctgcg cctgcgcggg tgacatgccc
     2401 ggcacttcca tcaggtcggc cacggtggcc cgctgcagct tggtcaactc gccgaagtgg
     2461 tccgtgaccg cggcggcgag ctcgtcgctg acgcgcggaa cccgggccag cagacgcatg
     2521 ccccgcgagg agaccgaacc gtcgaggtcg tcggctccgc cgaccgggtc gagggcgacc
     2581 gcggtgcggc gcaggtcgag cacgccgtcg tcgtcgaggc gagccatgaa ggtgtgcacg
     2641 tcttcgaccg cgctgctcac tggggtgtag tcgcggatga cgagatcgag ctcgtcgtcg
     2701 atctcgccgt acagctcgtc gagctggagg cgcagcagac gagcgtcgac acccagttcg
     2761 acgatcatcg tctcgatctc ttcggcgatg cggtgcacca tctcgccgcg ctgcaccacg
     2821 ctggccacgt cgcgcaggct gacgacgtct tcgacctctt gcgcggtcag gttcgacatc
     2881 gcgtcgtcga gacgcacctt gtagcgctcg agggtctgca gcgcctggtt ggcgcggtcg
     2941 agcaaccgcc cgacctcttg cagctggtgc ttgttggccc cggcgtagag gttgatcacg
     3001 ctcatctctt cgctgacact gaccacggga gcggacaagc tgcgcgccac ccgctcggcc
     3061 gtgcggtgcc gggtcccggt ctcggcagtg ggcaccgacg catcgggcaa caggtggacg
     3121 ttggcgcggg cgatgcggct gaggtcggcg ttgaggatga tcgccccatc catcttggcc
     3181 agttcgctga ggcgctgcgg tgagaacggc gcgtcgagca agaacccgcc cgagcagatc
     3241 tccagcaccg cggggtcgtc ggacagcacc agcagcgcac ccatcttggc ccggacgatg
     3301 cgttcgagcc cgtcgcgcaa gggtgtgccg ggagcgaccc gtgccaatgc gttgagcaac
     3361 tcgtcaggtc ggcgttgcat cggcattgcg tcatgatatt gggtgtgggc cgagcggcgt
     3421 tcagggcatg gttcgcaggg tgccgccagg gcgatcaccg ctggagagcc cggccaggcg
     3481 cagtgcctcg ggcagtgtcg aggcgcgcag cactcggatc ggcccgctgt gctctggggc
     3541 cgacgcgggc acgatcgccg tggcgaaacc cagccgggac gcttctccga ggcggcgatg
     3601 ggtctgagag acctggcgca gctctccggc cagacctact tcaccgcagg cgacgaggtt
     3661 ggcgggcagc ggttgaccga tcatggccga ggcgatcgcc aggcacagac ccaggtcgat
     3721 gcccggttcg gtgagcttga cgccgccgac gaccgagacg aacacctcgt gggccgcgac
     3781 cggcagcctc gcccggcgct cgatgacggc gatcagcatc gccagccggt tggcgtcgat
     3841 gccgtgggct gagcggcgca gggtgccacc gccgggcacg atgttggtca gtgcctgcac
     3901 ctcgaccagc agcgggcggt gtccttcgac cacgggcacc acggcagaac cggggacacc
     3961 ggtgcggcgg tcggccagga acagctggct cgggtcgggc accggcgcca ggccgtactc
     4021 gcccatttcg aacaagccga gctcgtcggt cggcccgaag cggtgcttgg ccgcgcgcag
     4081 cagccggagc gcgtggtgac gctcgccgtc gaaggagagc acggtgtcga ctacgtgttc
     4141 gagcacgcgc ggcccggcca gaccaccttc tttggtcacg tggccgacga gcaccatggt
     4201 gatgccgcgc cgcttggcct cttgcaccag ccggttggcg cagccgcgca cctgcgatac
     4261 cgagccgggt gccgagctga gctccgggtc ggcgatggtc tgaatgctgt cgatgacgac
     4321 gagctgtggt tgcaggtcgc cgagcgcggc gacgatgttg ggtagcgatg tctctgggag
     4381 cagccacagc tcgggtcgga tcgccgccaa ccgctcgccc cgcaaccgca cctgttgggc
     4441 gctctcttcg gccgacacgt acagggtgcg cccgacccag ctggccagca actgcagcaa
     4501 cagcgtgctc ttgcccacgc ccggctcgcc gccgaggagg gtgaccgaac cnnnnnnnnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4681 nnnncccgcc gccagcgaca ccggctcgtc ggccgcttcg acctcttcgg tcaacgagtt
     4741 ccacgcccca caagccgcgc accgccccga ccacttcgga tgagccgccc cacactcgga
     4801 gcaacggtgc acaactcggg acttcgccat gccgagcacc gtactcaggg ggtctcacac
     4861 agttcccgta acgccctcac gaccgaggac actcgtcctc gcccgcgacc gggccacagc
     4921 gcgcccttac ggcgtccgcc ggtcacccgc cgactggccc ggcggtgaat ctggcttacg
     4981 cggagtacgg agttaccgga tacaggggaa tccccgctcc gcggccgcgg gattgcccac
     5041 gccacgtcag ccaccgtgcc accgtgccat cgggtccggg tcgaagggga tgggggtggg
     5101 tcggtaggtt gggcaccgac gcgctggggg gcgcggagga acactcgggg ggttgtgatg
     5161 gaactggcca atgggacggt tgcggtggtg acgggggctg gtagcgggat cggtctcgcg
     5221 cttgccgagg cgctcgctcg cgcgggttgt tcggtggtgc tggccgacgt gcagcaggac
     5281 gccctcgacg cggcggcagc cacggtcggc gcgtacggcg tcgagacgct ctgcgtgcgc
     5341 accgacgtca gcaagctgga tcaggtcgag gcactggcgc aggcgacgct cgaccggttc
     5401 ggcgccgtcc acgtcgtgtg caacaacgcc ggtgtggccg gcgcgggtga tccgtgggtc
     5461 ggtccgnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ggtgtcgtcc acggcgtgcg
     5521 caccttcctg ccgcacatcg tcgcgtcggg gggtgggcac atcgtcaaca cagcctcgat
     5581 cgccggcctc ttcccagggt tcgccgcgcc gtacgacgcc agcaagcacg cggtggtcgc
     5641 gatgaccgag agcctgttca actcgttgca gatgtcgccc gtgcccgtcg gtgtgagctg
     5701 tctgtgcccc ggttgggtgc gtaccggcat tctcgacgca gaccgcaact ggccacccga
     5761 gttgggcgct cggcccgagg tcgacccgat ctcggccatc tcgcgtgagt acgtgcggcg
     5821 ggccatcgac gaaggcactc aggcggaaag tcgtcgcgcc aaggccgccg aagcccaggc
     5881 cgagctggtc gccgatctcg tcctcgatgc cgtgcgtgcc aaccgcttct gggtgttccc
     5941 caaccccgat ttcttggaga tcgcggtggc ccgcttccac accatcggcg aaggcctcga
     6001 cccgtcgcca cccgaacacg tccccggcat gcccccgcgc tcgaccatga tggccgaagt
     6061 cctggaagcc gtgatggcca ccatggcccc gacccccgcc cccgaacccg acagcgaccc
     6121 gccgacctga cgcgtgcttc gcccgtccca ccggtgggac gcggaacggc cccgggtttc
     6181 cccggggccg ttcgatcact ctggttgagc aggtgtgctc agaagtcttc catgccgccg
     6241 ccgggcatcg ccggggcgtc ttcgggcttg tccgcgacca cggcttcggt ggtcaggaac
     6301 aaggccgcga tcgaggctgc gttctgcagc gccgaccggg tgaccttggc cgcgtcgatg
     6361 acgccgagct tgacgaggtc ctcgtactcg ccggtggccg cgttgaggcc ctcgttgccg
     6421 gtgaggccct tgaccttctc gaccacgacg ccaccttcga ggccggcgtt ctcggcgatc
     6481 tgcttcagcg gggcttccag gctcttggcg accaggcggg caccggttgc ctcgtcaccc
     6541 gacagcgccg cagcggcctc gagcaccttg gcctgggcac gcagcagggc cacgccaccg
     6601 ccgggcacga cgccctcttc gatggccgcc ttggtggtgg agaccgcgtc ttcgatgcgg
     6661 tgcttcttct ccttgagctc gacctcggtg gccgcgccga ccttga
//
LOCUS       DAORNF010000077         6678 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_8497_length_6678_cov_1.621924, whole genome shotgun sequence.
ACCESSION   DAORNF010000077 DAORNF010000000
VERSION     DAORNF010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 6678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6678
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8497_length_6678_cov_1.621924"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1783)
                     /locus_tag="PLV68_04490"
     CDS             complement(<1..>1783)
                     /locus_tag="PLV68_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00533.1"
                     /translation="GITDVRIIEKGGDFGGTWYWNRYPGAMCDTASMVYMPLLEETGH
                     MPTEKYAHAPEILEQCRRIGTQYGLYDHALFHTEVTGLEWDDSRSRWIVRTNRGDEFT
                     AKYLGMGTGPLHVPKLPGIPGIELFEGHSFHTSRWDYDYTGGDPNGAPLDKLADNRVA
                     IIGTGATAVQAIPHLAAVCATLYVFQRTPSSVDVRDNHPIDPDWFATINTPGWQKRWL
                     ENFTANQAGGAADEDLVQDGWTDLARRIRERIMTLPVEQRSIENMLIAYEDADFEKMS
                     EIRDRIDRIVTDHDTAQQLKAWYNQLCKRPCFHDEYLQAFNEPGTRLVDTDGRGVERI
                     TANAVVVNGTEYEVDCIIYASGFEVGTPFEQRNGYDPIGRDGETLSQHWAEGMRTKHG
                     THVHGFPNLFIVQPTQGANLISNVPHNLTEAGQTIAMTIAHAEANGFGQVEVTAEAEQ
                     EWIDLLRTGAGSMNGKEAVIGTVLMLIGQNSRTVAEDVSAKIAVGRLPGWAHRVFPLP
                     RRVAQRRPLRRTRVPLSRVSPSAGCCPVFGPPALRSGRDVSDNRRMLSHYQQARQELL
                     SPGSPFAVETIEVRGVPIKAFSSAPASM"
     assembly_gap    1784..1952
                     /estimated_length=169
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2168..4051)
                     /locus_tag="PLV68_04495"
     CDS             complement(2168..4051)
                     /locus_tag="PLV68_04495"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF002937.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030729 - acetoacetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HRE00534.1"
                     /translation="MGDNLLARLAAQVGASDYRQLHAWSIADLDRAWATACDAVGVTW
                     IDRPTRVLADATMPGAEWFPGGTLNLTQHVLARAAVAPDEVAVIEHSQTRPVQMLTWA
                     QLADAVHRVAGGLAGRGIGRHSRVGAYTPNTVETLVAFLATAQLGAQWSSCAPEFGPK
                     AVVDRFGQFRPDVLLTVDGYQYGAKRIDRVATSVEIVAQLPGAPAVIPIRYLGTGDDG
                     FGALLAADPITTYAPMRYRDPLYVLYSSGTTGLPKPIVHSHVGIAVEHMVVHQLHHDL
                     GPGERFFWFTTTGWMMWNYLVSALASGASIVLFEGDPAAADLSTLWQVAEQTDTTVFG
                     MSAGFIMNCRKAGLTPPRGRIREIGSTGAPLPIEGDLWLRERLPGVAVNSISGGTDVC
                     SAFVGANPMTPTAPGTLGGSLFAREAAAFDEHGARCPTGERGELVITTPMPSMPLAFL
                     GDDDGSRLFHAYFDRFPGVWCHGDWVTFAADGSCVISGRSDATLNRGGVRLGTADFYA
                     VVDDLAEVRDSLVVHVDEPRDGVERDQLFLFVQLQPDLAVTDELRATIKAALRTALSP
                     RHVPDVIEAVPSVPRTLSGKRLEVPVKQILLGRDYDGVLSKESLANPAAIDWFVTYAA
                     ANG"
     gene            complement(4057..4701)
                     /locus_tag="PLV68_04500"
     CDS             complement(4057..4701)
                     /locus_tag="PLV68_04500"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRE00535.1"
                     /translation="MSNNVWLQMIERNPGHSQWYIERFRTMAAEGADLDGEARMIDAM
                     VGRGARILDAGCGPGRLGGRLHHLGHRVVGVDLDPALIAAAEADHPGPTWLTGDLSQL
                     DLATPDLAAAGVLAEFDVIVSAGNVMTFLDPATRQSVLDRLAAHLAPDGRLVIGFGAG
                     RGYEFDDFFAHAAVAGLREQLRLSTWDLRPFGPDSNFLVAVLGRPDLDRGDLGG"
     gene            4771..5643
                     /locus_tag="PLV68_04505"
     CDS             4771..5643
                     /locus_tag="PLV68_04505"
                     /inference="COORDINATES: protein motif:HMM:TIGR03620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03620 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HRE00536.1"
                     /translation="MTSRLGRTGVWSSNLRYGDAAETAAHAAELEQLGYSALWVPDIG
                     GDLWSPLANLLGATTTTTIATGILNIWMHTAEETAGQHADLVAAHGPRFMCGLGISHQ
                     RLIDGTKEPGAYQRPIATMTAYLDGLDSAATPLAAGDRMIAALGPKMLELARARSAGS
                     HPYLVTPELTAAARAGIGADGLVAAEQGVVLETNPSTAREIARLHLAPYLGLPNYSNN
                     WKRQGFTDDDIADGGSDRLVDALVVWGDEATIAERVQQHRDAGADHVCIQALTATPRE
                     FPIAQWRTLAPALT"
     assembly_gap    5882..5910
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5929..>6289
                     /locus_tag="PLV68_04510"
     CDS             5929..>6289
                     /locus_tag="PLV68_04510"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRE00537.1"
                     /translation="MVEFAPGLGATARLLLQHNPSSYTAVDADEEAAATVRAVVEPSG
                     GTVLVGTAQHVPLPDDDATAVVGEAMLTMQSDTNKAGIIAEAARLLAPGGRYAIHELC
                     LVPDDIDDATAQAVWDDL"
     assembly_gap    6290..6424
                     /estimated_length=135
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1042 a         2161 c         2160 g          982 t
ORIGIN      
        1 catcgaggcg ggcgccgagg agaaggcctt gatcggcacg ccccgcactt cgatcgtctc
       61 gaccgcgaac ggcgaaccgg gtgagaggag ttcttggcga gcctgttgat agtgcgagag
      121 catccggcga ttgtcactga cgtcacgtcc cgaacgcaaa gcgggcggac cgaagaccgg
      181 acagcagcca gcggaaggcg agacgcgact cagcggaacg cgagtccggc gaagcggccg
      241 tcgctgcgcc actcgtcgag gtagcggaaa tacgcggtgg gcccagccgg gtaaccgacc
      301 gacagcgatt ttcgccgaga catcctcggc cacggtgcgg ctgttctgac cgatcagcat
      361 gagcacggtg ccgatcaccg cctccttgcc gttcatgctg cccgcaccgg tgcgcagcag
      421 gtcgatccac tcctgctcgg cctcggcggt cacctcgacc tggccgaagc cgttcgcctc
      481 ggcgtgcgcg atggtcatcg cgatcgtctg gcccgcctcg gtcaggttgt gcgggacgtt
      541 ggagatcagg ttggcaccct gggtcggctg cacgatgaac aggttgggaa acccgtgaac
      601 gtgggtgccg tgcttggtgc gcatcccctc ggcccagtgc tgcgacaacg tctcgccgtc
      661 acgcccgatc gggtcgtagc cgttgcgctg ttcgaacggc gtgccgactt cgaacccaga
      721 cgcgtagatg atgcagtcga cctcgtactc ggtgccgttc accaccaccg cattggcggt
      781 gatccgctcg acgccgcggc cgtcggtgtc gacgaggcgc gtacccggct cgttgaacgc
      841 ctggagatac tcgtcgtgga agcacggccg cttgcagagc tggttgtacc aagccttgag
      901 ctgctgcgcc gtgtcgtgat cggtgacgat gcggtcgatc cggtccctga tctcgctcat
      961 cttctcgaag tcagcgtcct cgtacgcgat gagcatgttc tcgatggagc gctgctcgac
     1021 gggaagggtc atgatccgct cgcggatgcg gcgggcgagg tcggtccagc catcctgcac
     1081 caggtcttcg tcggcggcgc cgccggcctg gttggcggtg aagttctcca accatcgctt
     1141 ctgccagccc ggcgtgttga tggtggcgaa ccagtcggga tcgatcgggt ggttgtcgcg
     1201 gacgtcgacc gacgacggcg tgcgctggaa cacgtacagc gtggcgcaaa cagcagcgag
     1261 atgaggaatg gcttggacgg cggtggcacc cgtgccgatg atcgctaccc gattgtcggc
     1321 caacttgtcg agcggcgcac cgttcggatc gccaccggtg tagtcgtagt cccaccgcga
     1381 cgtgtgaaac gagtgaccct cgaacagctc gatgccaggg atgcccggca gcttcggcac
     1441 gtgcaacgga ccggtaccca tgcccaggta cttggcggtg aactcgtcac cgcggttggt
     1501 gcgcacgatc cagcgcgacc gcgagtcgtc ccactccaag ccggttactt ccgtatggaa
     1561 caacgcatgg tcgtacaacc cgtactgggt gccgatgcga cggcactgtt cgaggatctc
     1621 gggcgcgtgc gcgtacttct cggtgggcat gtgcccggtc tcttcgagta acggcatgta
     1681 gaccatcgac gccgtgtcgc acatcgcgcc ggggtagcgg ttccagtacc aggtgccgcc
     1741 gaagtcgccg cccttctcga tgatccgtac atcggtgatg ccgnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngtcgggac gcagccgctt gtcgcgctcg
     1981 gctgcgtact tctccttcag cgcctgcttg tcgatcgtgg tcacacgcac ccccgtgctc
     2041 gtcgcccggt cgcccaggtg gcgaaccggt agcgggtgac caattgtcta cggggccgac
     2101 cccgccccgc cgaagccgga gcgccggatc ggtatgtgat cgctgaaggt cggttgctgg
     2161 tcgggagtca gccgttcgcg gccgcgtagg tgacgaacca gtcgatggcg gcggggttgg
     2221 cgagggactc tttgctcaac acgccgtcgt agtcacggcc gagcaggatc tgtttgaccg
     2281 gcacttccag ccgtttgccc gacagggtgc gaggcacgct gggcacggct tcgatcacgt
     2341 cggggacatg acggggcgac agggcggtgc gcagggcggc cttgatcgtg gcccgcagtt
     2401 cgtcggtcac ggcgagatcg ggttgcaact gcacgaacaa gaagagttgg tcgcgctcga
     2461 ccccgtcgcg cggctcgtcg acgtgcacca cgaggctgtc gcgcacctcg gccagatcgt
     2521 cgaccacggc gtagaagtcg gccgtcccca ggcgcacgcc gccacggttg agcgtggcgt
     2581 cactgcgccc ggagatgacg cagctgccgt cggccgcgaa cgtcacccag tcgccgtggc
     2641 accacacgcc gggaaagcgg tcgaagtacg cgtggaacag ccgcgacccg tcgtcgtcac
     2701 cgagaaacgc cagcggcatc gacggcatcg gcgtggtgat caccaactca ccacgttcgc
     2761 ccgtcgggca gcgggcgccg tgctcgtcga acgctgccgc ctcgcgggcg aacagcgacc
     2821 cgccgagggt gccgggcgcg gtgggtgtca tcgggttggc gcccacgaag gcgctgcaca
     2881 cgtcggtgcc gccggagatc gagttgaccg ccacacccgg cagtcgttcg cgcaaccaca
     2941 gatcgccctc gatcggcaag ggcgctcccg tcgagccgat ctcgcggatg cggccccgcg
     3001 gcggcgtcag cccggccttg cggcagttca tgatgaaccc cgccgacata ccgaacaccg
     3061 tcgtgtcggt ctgctcggcg acctgccaca gcgtggacag atcggctgcg gcgggatcgc
     3121 cctcgaacag cacgatcgac gcgccactgg ccagggccga gaccaggtag ttccacatca
     3181 tccagccggt ggtggtgaac cagaagaacc gctcgccagg gccgagatcg tggtgcagtt
     3241 ggtgcaccac catgtgttcg acggcgatgc cgacgtggct gtgcacgatc ggcttgggca
     3301 gaccggtcgt accggagctg tacaacacgt acagcgggtc gcgatagcgc atcggcgcat
     3361 acgtggtgat cgggtcagcg gcgagcaggg caccgaaccc gtcgtcaccc gtgccgaggt
     3421 agcgaatggg gatcaccgcc ggcgcgccag gcaactgggc gacgatctcg actgaggtcg
     3481 ccacgcggtc gatccgtttc gcgccgtact gatagccgtc gacggtcagg agcacgtcgg
     3541 ggcggaactg tccgaagcga tcgaccacgg ctttcgggcc gaactcgggc gcacaactcg
     3601 accactgcgc gccgagttgg gccgtggcca agaacgcgac caacgtctcg acggtgttcg
     3661 gtgtgtaggc accgaccctg ctgtggcgac cgatgccgcg cccggccaga cccccggcga
     3721 cgcggtggac tgcgtcggcc aactgcgccc acgtgagcat ctgcaccggg cgggtctggc
     3781 tgtgctcgat caccgcaacc tcgtccggcg cgaccgcggc gcgggccagc acgtgctggg
     3841 tgaggttcag ggtgccgccc ggaaaccact cggcaccagg catcgtcgcg tcagcgagca
     3901 cacgggtcgg ccggtcgatc caggtgacgc cgaccgcgtc gcacgcggtg gcccaggcgc
     3961 ggtcgagatc ggcgatcgac caagcgtgca gttggcgata gtcggacgcg ccgacctgtg
     4021 ccgccagtcg agccaggaga ttgtcaccca tcgtcgtcag ccgcccaagt caccacgatc
     4081 gaggtccggg cggcccagca ccgcgaccag gaagttgctg tccggtccga acgggcgcag
     4141 gtcccaggta gacaggcgca actgctcacg cagaccggcg acagcggcgt gggcgaagaa
     4201 gtcgtcgaac tcgtagccgc ggccagcacc gaagccgatc accagccgac cgtcgggggc
     4261 caggtgggcg gcgaggcgat cgagcaccga ctggcgggtc gccggatcga ggaaggtcat
     4321 cacgttgccg gccgagacga tgacgtcgaa ctccgccagc acgcctgcgg cggccagatc
     4381 aggggtggca aggtcgagct gggacagatc gcccgtgagc catgtcggtc cggggtgatc
     4441 ggcctcggcc gccgcgatca gagctggatc gaggtcgacc ccgaccaccc ggtggcccag
     4501 gtggtgcaac cggccgccaa ggcgacccgg cccgcacccc gcgtcgagga tgcgtgcgcc
     4561 gcgcccgacc atggcgtcga tcatccgcgc ttcgccgtcg aggtcggcac cctcggccgc
     4621 catcgtgcgg aaccgctcga tataccactg cgagtgcccc gggttgcgtt cgatcatctg
     4681 cagccacacg ttgttgctca ccacaccgac cgtaccgctc gcctcccgtt cggcgaaacc
     4741 gatccgccgc gatcatggca agctgggggc atgacgtcac gacttggcag gaccggggta
     4801 tggagttcga atctgcgcta cggcgacgcc gcagagaccg cggcccacgc ggctgaactc
     4861 gagcagttgg gctactcggc gctgtgggtg ccggatatcg gcggcgacct gtggagtccc
     4921 ctcgccaacc tgctcggcgc caccacgacc accacgatcg cgaccggcat cttgaacatc
     4981 tggatgcaca cggccgagga gaccgccgga cagcacgccg acctcgtcgc tgctcacgga
     5041 ccacggttca tgtgtggcct gggcatcagc caccagcggc tgatcgacgg caccaaggag
     5101 cccggcgcgt accagcggcc gatcgccacc atgacggcgt atctcgacgg cctcgacagc
     5161 gccgccacac cgctggccgc gggtgaccgg atgatcgctg cgctcgggcc gaagatgctc
     5221 gagctggccc gcgcccgctc ggccggctcg cacccctatc tcgtcacccc cgagctcacc
     5281 gccgcggcgc gcgctggcat cggtgccgac gggttggtcg cggccgagca gggcgtcgtg
     5341 ctcgagacca atcccagcac cgcccgcgag atcgcccggc tccatctcgc gccctacctc
     5401 ggcctgccca actactccaa caactggaag cgccagggct tcaccgacga cgacatcgcc
     5461 gatggaggca gcgaccggct ggtcgacgcg ctcgtggtgt ggggcgacga ggcgaccatc
     5521 gccgagcgcg tccaacaaca tcgtgacgcc ggtgccgacc acgtctgcat ccaggccttg
     5581 acggccacgc cgcgcgagtt cccgatcgcc cagtggcgca ccctcgcccc cgccctgacc
     5641 tgacctgagg gcagcgccgg caatccggga cttttggcac gggacgggtt cctctgcggt
     5701 tttctagagt tacctctgtg aaccagtccg aacagcgcac gttgcgcgcg gctgtcagtc
     5761 acaccacacc gtcagccgag gaggttgccc gatgacacgc gacacgattc ccaacgagaa
     5821 ggtacccggc cactggttgc tggcccggat gggcaagagg gtgctccggc cgggcggcgc
     5881 gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gttaccacgg ctgatcgagt ggtcgagttc
     5941 gcgcctgggc tcggtgccac ggccaggttg ttgttgcagc acaacccgtc cagctacacc
     6001 gccgtcgacg ccgacgagga ggcggctgcc accgtgcgcg ccgtggtcga gccgtctgga
     6061 ggcaccgtgc tggtcggcac cgcccagcac gtgccgctgc ccgacgacga tgccactgcc
     6121 gtggtcggcg aggcgatgtt gaccatgcag agcgacacca acaaggcagg catcatcgcc
     6181 gaagcggcgc gactgctcgc ccccggcggc cgctacgcca tccacgagct gtgcctggtg
     6241 cccgacgaca tcgacgacgc cacggcgcag gcggtgtggg acgacctgtn nnnnnnnnnn
     6301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6421 nnnncgccga cgagggcctc gtcgcggcgg tgaagttctt cgtcaacgtg gcccgcgatc
     6481 gcaccgcccg cgagcgggtc aagacgatgc ggggggtgtt ccgcaagtgg tccaagcacc
     6541 tcggtgcggt ggcgctggtc gcctcccgca acgacgtgtg acgcactgcg cgtcatccgc
     6601 tccggttgac gcgcagtcgg gccagagggg gagaatggtc tcctcatgag tcacccccac
     6661 ccccacgatc acccgcac
//
LOCUS       DAORNF010000078         6660 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_8528_length_6660_cov_2.126117, whole genome shotgun sequence.
ACCESSION   DAORNF010000078 DAORNF010000000
VERSION     DAORNF010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 6660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6660
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8528_length_6660_cov_2.126117"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1221)
                     /locus_tag="PLV68_04515"
     CDS             complement(<1..1221)
                     /locus_tag="PLV68_04515"
                     /inference="COORDINATES: protein motif:HMM:NF014731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HRE00538.1"
                     /translation="MEHGSHGGEAVAATTNGGDGDDEPSSVTQDADESHAGTGHDGHA
                     PASGGGGHAALGTGAMALGALGIVYGDIGTSPLYAFRESFEHHGLDTHNPANALGVAS
                     VVFWALVVIISIKYLIFVMRADNHGEGGILALTAMIMPKGAMTRRAAIVVTLGVFGTA
                     LLYGDGLISPAISVLSAVEGLDRVSPSLADLVVPIAIAILVMLFLVQRRGTGGIGRVF
                     GPVMVVWFSAVGVLGLSQIVRLPSVLKAASPMYAFDWFRAAPFNSFLTMGSIFLVVTG
                     GEALYADMGHFGRRPIQLTWYAIVMPCLMLNYFGQAALILRNPDVEMKSLFYEMAPEW
                     ASVPLVVLATMAAVIASQAMISGAYSMTVSAVQLDYLPRVKIMHTSSSHRGQVYVPLV
                     NWALMVGCIGLVVLF"
     gene            complement(1320..3296)
                     /locus_tag="PLV68_04520"
     CDS             complement(1320..3296)
                     /locus_tag="PLV68_04520"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00539.1"
                     /translation="MSRFSRVVLATLFAGGGLVAVAAAPASAGTASQSTLTLIVRTIG
                     GEGTFDYSGSGFADFTLSTESESMYDDTKAFALPSGNVLIQQDNAEGSEGFVLRDVYC
                     EDADDSEYEGANWLDSDPDFFVPLAPNHQVRCVITNIVGPAVTIDKWAGGDADLSQFI
                     FTVTPVSFDDEAVDEGWYIARDDLTLLGEPVLTGSDPSEAEAIFGDGSIAEDALIGSL
                     PYDTESEATAYFLIEEAQVPGWILNWVDCYGEYYTYDTDNGVVVGVNASTLVQCEFDN
                     NGIAPDLELVKTTNPGPHVAGGLVEYRFTITNVGGSFDNFQQPSAEDEPETYSLDDEL
                     PAGQTWNAVPEGCTGTGTAILSCELPLDALSNSGDSVTVSVWATIAPDVASGRYDNMA
                     YISWYRHDEPCAWEWENWAEAEVSAVSEDCTLLPCGALDPRDDNFESLAYSANPANNV
                     SCAAIDVIRAAAVTLAKTDNAGAGVTPGASFTYTLQAANTGPSSVLAGSRLVDDLPDG
                     LTLTSINAGSGWTCNNADPVVCDYANGIAPGANATPVTITVSLSASYASSSITNVATF
                     TAPYDVEAAVPTAEAAGDLITAAVSATRVTPVTQAEATTTSQATTTTAAGASPTPPGT
                     LPELGSSTGWLTTMAALLLALGGVVLITRRRRTV"
     gene            complement(3470..>3726)
                     /locus_tag="PLV68_04525"
     CDS             complement(3470..>3726)
                     /locus_tag="PLV68_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00540.1"
                     /translation="RYVCIDTSTRVVAATGEPAAVRPQQQFLQLWGIEQLVTEGQEYW
                     NAHAVRPGVQALLMRSRVREAEALCDPAGLGNFTVVEYRR"
     assembly_gap    3727..3763
                     /estimated_length=37
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3764..4546)
                     /locus_tag="PLV68_04530"
     CDS             complement(<3764..4546)
                     /locus_tag="PLV68_04530"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HRE00541.1"
                     /translation="MPHSAAEHIAAAVDAAGGALRFDRFLSLALYGPGGFYTTDAADG
                     GGRAGRRGDFITSAEVGPLFGAVLARYIDAVWDELGQPATMTVVEGGAGPGTLARSIR
                     NARPRCLDALHYVAVEVSAAQRALHPDGVESRATMPTAPFRGVLLANELLDNIPFRLA
                     VMDGGWREAYVVTDGDRFAEVLRPFEGDIDRDIDRLPARAAHGARAPIQVDAGEWLAG
                     ALGLLEAGRAVVIDYMSATTAGLVLRPWREWLRTYRNHQRGGH"
     gene            complement(4527..6047)
                     /locus_tag="PLV68_04535"
     CDS             complement(4527..6047)
                     /locus_tag="PLV68_04535"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HRE00542.1"
                     /translation="MLSSILAAAGDFKAPHIDYHAIAPEAIVAAGICLVLVVDLFVAE
                     SKKWITATLSGLVLLGAMIPVVTLAVIGDASRSLFDGRYVVDEFALIMKALFLLTGYV
                     VVLLSTNEIEEGGYHQGEYYTLLLSSILGMVMMASARDLISIFVALEFLSIPVYLLAS
                     WRKRDGKSNEAGMKYFLIGVFASGVMLYGMSLLFGVTGTTKLSELGAVVAAEGRFGSV
                     ETLAIVFVIVGFAFKVSAVPFHNWAPDTYEGAPTPITAFLSVGAKAGGFVALVSLVYL
                     SFPQAKDVWQPFMFAMAALTMTIGNVLALKQTNMVRMLAYSSISQGGFILMPFVVAGT
                     SAAEASLKTVVAYLLVYASTNLGMFAVVIAVSRKTRSGDIASFGGLIHYAPGLAALMT
                     FFLASLAGIPPFGGWFAKLSAFRAVLEADTGLGYAIAIIGAVNTVIAFGYYGSVMREI
                     WMKPIPDGDDRPIRTPSALTAALAITAVATLVLGILPQSVLRFGDLVDLTGAALGR"
     gene            complement(6051..>6660)
                     /locus_tag="PLV68_04540"
     CDS             complement(6051..>6660)
                     /locus_tag="PLV68_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HRE00543.1"
                     /translation="LGFNAAIFGMVAHGLITGMLFFVAGSIKERYHTLEIKRLGGLLL
                     QMPKMGWILGFAAMASLGLPGLAGFWGEFPAIVSTYNPGGGLNHSVFRVYMVIAAMGT
                     VLAAGYLLWLFQRVAFGEPKPEFSGEVHAHSVGAAANHGHDGHGHGDGDDHHSDIHDV
                     SIYEWIAWTPFLIGIVLFGVYPQLLFRIIDPASQVALKAFGG"
BASE COUNT         1168 a         2305 c         2169 g          981 t
ORIGIN      
        1 gaacagcacc accagcccga tgcagccgac catcagcgcc cagttgacga gcggcacgta
       61 cacctgaccg cggtgcgacg acgaggtgtg catgatcttg acgcgcggca ggtagtcgag
      121 ctgcacggcc gagaccgtca tcgagtaggc gcccgagatc atcgcctggc tggcgatgac
      181 ggcggccatc gtggccagca ccaccaacgg cacgctggcc cactccggcg ccatctcgta
      241 gaacaggctc ttcatctcga cgtcagggtt gcgcaggatc agcgcggcct gaccgaagta
      301 gttcagcatc aggcacggca tcacgatcgc gtaccaggtg agctggatcg gtcgccgccc
      361 gaagtggccc atgtcggcgt acaacgcctc gccgccggtg accaccaaga agatcgaacc
      421 catcgtcagg aacgagttga acggggcggc gcggaaccag tcgaacgcgt acatcgggct
      481 ggccgccttg agcacgctgg gcagccggac gatctggctg aggccgagca caccgactgc
      541 gctgaaccac accaccatca ccggcccgaa cacccgcccg atcccgcccg tgccgcggcg
      601 ctgcaccagg aacagcatca ccaggatggc gatcgcgatc ggtacgacca gatcggccag
      661 cgacgggctc acccggtcga gaccttcgac cgcgctcaac accgagatcg ccgggctgat
      721 cagtccgtcg ccgtagagca gggcggtgcc gaacacgccg agggtgacca cgatcgccgc
      781 tcggcgggtc atcgccccct tgggcatgat catcgccgtc agggcgagga tgccgccctc
      841 gccgtggttg tcggcgcgca tcacgaagat caggtatttg atcgagatga tcaccaccaa
      901 cgcccagaac accaccgacg cgacgccgag cgcgttggcc gggttgtggg tgtcgagacc
      961 gtgatgctcg aacgattcac ggaacgcgta cagcgggctg gtgccgatgt cgccgtagac
     1021 gatgccgagc gcgccgaggg ccatcgcccc cgtgcccaac gcggcgtggc cgcctccacc
     1081 gctggccggc gcatggccgt cgtggccggt tccggcgtgc gactcgtcgg cgtcctgggt
     1141 tactgacgag ggctcgtcgt caccgtcgcc accgttggtg gtcgccgcga cggcctcgcc
     1201 accgtgtgat ccgtgctcca tagcggacgc gacgctacct ccgccccggc atgttgagcg
     1261 ccgcggagtg cggcgcccaa cacccggtca gcgggtcgag cgaggtcaga caggtgggct
     1321 cagacggtgc ggcggcgacg cgtgatcagg accaccccac cgagggccag caacagcgcg
     1381 gccatcgtcg tcaaccaacc tgtgctggag cccaactctg gcaacgtgcc gggcggcgtg
     1441 ggtgacgcgc cggcagccgt cgtggtggtg gcctgcgacg tcgtcgtcgc ctcggcttgc
     1501 gtcaccggcg tcacgcgagt ggcgctgacc gcagccgtga tcagatcacc agcagcctcc
     1561 gcagtaggca ccgcggcctc gacgtcgtac ggcgcggtga aggtcgccac gttggtgatc
     1621 gacgacgatg cgtacgacgc gctcaacgac accgtgatcg tcaccggcgt ggcgttggcc
     1681 cccggagcga tcccgttggc gtagtcgcac acgaccggat cggcgttgtt gcacgtccag
     1741 ccgctgcccg cgttgatcga cgtcagcgtc agaccgtcgg gcagatcgtc gaccagtcgc
     1801 gaaccagcca gcaccgacga cggaccggtg ttggccgcct gcagggtgta ggtgaacgac
     1861 gcgcccggcg tcacgccggc acccgcattg tcggtcttgg ccagcgttac ggccgcagcc
     1921 cggatcacgt cgatggccgc acaactgacg ttgttggccg ggttcgcgct gtacgccaag
     1981 gactcgaagt tgtcgtcgcg cgggtccagc gcgccacacg gcagcagcgt gcaatcttcg
     2041 ctcaccgccg acacctcggc ttcggcccag ttctcccact cccaggcgca gggctcgtcg
     2101 tggcgatacc acgagatgta cgccatgttg tcgtaacgac ccgacgccac gtcgggcgcg
     2161 atcgtggccc acaccgacac ggtgaccgag tcgccactgt tggacagcgc gtcgagcggc
     2221 aactcgcacg acaagatcgc cgttccggtg ccggtgcacc cttcgggaac cgcgttccag
     2281 gtctgcccgg cgggcaactc gtcgtccagc gagtaggtct ccggctcgtc ctcggccgag
     2341 ggctgctgga agttgtcgaa cgatccgccc acgttggtga tcgtgaaccg gtactcgacc
     2401 agaccacccg ccacgtgcgg cccggggttg gtcgtcttca ccaactccag atcgggggcg
     2461 atcccgttgt tgtcgaactc acattgcacc agggtcgagg cgttgacgcc gacgaccact
     2521 ccgttgtcgg tgtcgtacgt gtagtactcg ccgtagcagt ccacccagtt caggatccag
     2581 ccgggcacct gcgcttcctc gatcaggaag tacgcggtcg cttcgctctc ggtgtcgtag
     2641 ggcagcgagc cgatcagtgc atcctccgcg atcgacccgt ctccgaagat cgcctccgcc
     2701 tcgctcggat cagacccggt gagcacgggt tcaccgagca gggtcagatc gtcgcgagcg
     2761 atgtaccaac cttcgtcgac ggcctcgtcg tcgaagctga ctggcgtgac cgtgaagatg
     2821 aactggctga ggtcggcgtc acccccggcc cacttgtcga tcgtcaccgc cggaccgacg
     2881 atgttggtga tcacgcaccg cacctgatgg ttgggtgcca gagggacgaa gaagtcgggg
     2941 tccgagtcca gccagttcgc gccctcatac tcggagtcgt ccgcgtcctc gcaatacacg
     3001 tcacgcagca cgaagccctc cgacccctcg gcgttgtcct gctgaatcag gacattgccc
     3061 gatggcaggg cgaaggcctt cgtgtcgtcg tacatcgact cgctctcggt cgacaacgtg
     3121 aaatcggcga atccgctgcc gctgtagtcg aacgtgccct ccccgccgat ggtgcgcaca
     3181 atcagcgtca gcgtcgactg cgaggcagtc cccgcgctgg cgggcgcagc cgcgaccgcg
     3241 accagaccac ccccagcgaa caaggtcgcc aagaccactc gtgaaaatcg actcatcgtg
     3301 tgtgcccttc tgtcgtcgcc gtcacccacg cgccacgcct gcccggcgcc tcgttactga
     3361 cggagtccct gccggcactg tagagcaccg cgtcataccg gacggacatg acggcacacc
     3421 gccgggacgc gggcgagacg tggcactcgc gcccggcgat ggctcgcggt cagcggcggt
     3481 attcgaccac cgtgaagttg cccaggcccg ccgggtcgca cagcgcttcg gcctcgcgca
     3541 cccggctgcg catcaacagc gcctgcacac cggggcgtac cgcatgggcg ttccagtact
     3601 cctgcccctc ggttaccagt tgctcgatgc cccacagttg caggaactgc tgctgcgggc
     3661 gcaccgcggc cggctcgccc gtggcggcga ccacacgcgt gctggtgtcg atgcagacgt
     3721 agcgccnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngtgcccg ccgcgctggt
     3781 ggttgcggta ggtgcgcaac cactcccgcc acggccgcag caccaacccc gccgtggtcg
     3841 cggacatgta gtcgatcacc actgcccgcc ccgcctccag cagcccgagc gcaccggcga
     3901 gccattcgcc cgcgtcaacc tggatcgggg cgcgagcgcc gtgcgcggcg cgggccggca
     3961 ggcgatcgat atcacggtcg atgtcgccct cgaacgggcg gagcacttcc gcgaaccggt
     4021 cgccgtcggt gacgacgtat gcctcgcgcc agccgccgtc catcaccgcc aaccgaaacg
     4081 ggatgttgtc gagcagttcg ttggccagca gcacgccgcg aaacggcgcc gtcggcatcg
     4141 tcgctcgcga ctcgaccccg tcgggatgca gagcacgctg cgcggctgag acctcgaccg
     4201 cgacgtagtg cagcgcatcg agacagcgcg ggcgggcgtt gcggatcgag cgcgccagcg
     4261 tgcctggtcc cgccccgccc tcgaccacgg tcatcgtcgc gggctggccc aactcgtccc
     4321 acaccgcgtc gatgtagcgc gccaacaccg cgccgaacaa cgggccgacc tcggcgctgg
     4381 tgatgaagtc gccgcggcgc ccggctcgcc cgccgccgtc ggcggcatcg gtggtgtaga
     4441 agccacccgg cccgtagagc gcgagcgaca ggaaacgatc gaagcgcagc gccccgcccg
     4501 ccgcgtcgac ggccgcggcg atgtgctcag cggccgagtg cggcacccgt cagatcgacc
     4561 aggtcgccga agcgcagcac cgactgcggc aggatgccca gcaccagcgt ggccaccgcg
     4621 gtgatcgcca gcgcggcggt cagcgcagag ggcgtgcgga tcgggcgatc gtcgccgtcg
     4681 gggatgggct tcatccagat ctcacgcatg accgagccgt agtagccgaa cgcgatcacg
     4741 gtgttgaccg caccgatgat ggcgatcgca tagccgaggc ccgtatcggc ttcgagcacc
     4801 gcccggaacg cgctgagctt ggcgaaccag ccgccgaagg gcgggatacc ggccagcgaa
     4861 gcaaggaaga acgtcatcag cgcggccagg cccggggcgt agtggatcag gccaccgaag
     4921 ctggcgatgt cgccgctgcg ggtcttgcgc gacaccgcga tcaccacggc gaacatgccg
     4981 aggttggtgc tggcatagac caacaggtag gcgaccaccg tcttgagcga cgcctcggct
     5041 gcgctggttc cggcgaccac gaacggcatc aggatgaacc cgccctggct gatggacgag
     5101 taggccagca tgcggaccat gttcgtctgc ttcagcgcga gcacgttgcc gatggtcatg
     5161 gtcagcgccg ccatcgcgaa catgaacggc tgccacacgt ccttggcctg ggggaagctg
     5221 aggtacacca gcgacaccag ggcgacgaag ccgcccgcct tggcgccgac cgacaagaac
     5281 gcggtgatcg gggtgggcgc gccttcgtag gtgtcgggcg cccagttgtg gaacggcacg
     5341 gcgctgacct tgaacgcgaa accgacgatg acgaacacga tcgccagcgt ctcgaccgaa
     5401 ccgaaccggc cctcggccgc gaccaccgca ccgagctcgg acagcttggt ggtaccggtc
     5461 acgccgaaca gcaggctcat cccgtacagc atcaccccgc tggcgaacac gccgatcagg
     5521 aaatacttca tgcccgcttc gttgctcttg ccgtcgcgct tgcgccacga cgccagcaga
     5581 tagacgggga tgctgaggaa ctccagcgcg acgaagatgc tgatcaggtc acgggccgag
     5641 gccatcatga ccatgccgag gatgctggac agcagcaggg tgtagtactc gccctggtgg
     5701 tagccgcctt cttcgatctc gttggtggac agcagcacca ccacgtaccc ggtcagcagg
     5761 aacagcgcct tcatgatcag cgcgaactcg tcgaccacgt accgcccgtc gaacagcgaa
     5821 cggctggcgt caccaatgac agccagggtg accaccggga tcatcgctcc cagcagcacc
     5881 aggcccgaca gggtggcggt gatccacttc ttcgactcgg ccacgaacag atcgaccacc
     5941 agcaccaggc agatgcctgc cgcgacgatc gcctcggggg cgatcgcgtg gtagtcgatg
     6001 tgcggcgcct tgaagtcgcc ggccgcggcc aggatggacg agagcatgcg tcagcctccg
     6061 aacgccttca gcgccacctg ggaagcggga tcgatgatgc ggaacaggag ctgcgggtag
     6121 acaccgaaca acacgatgcc gatcaggaac ggcgtccatg cgatccactc gtagatcgag
     6181 acgtcgtgga tgtcgctgtg gtggtcgtcg ccatcaccgt gaccgtgacc gtcatgtccg
     6241 tggttggccg ctgccccgac cgagtgggcg tgcacctcgc cgctgaactc gggcttcggc
     6301 tcgccgaagg cgacccgctg gaacagccac agcaggtacc cggcggcgag caccgtgccc
     6361 atcgccgcga tcaccatgta cacccggaac acgctgtggt tgagaccgcc gcccgggttg
     6421 taggtggaca cgatcgccgg gaactcaccc cagaacccgg ccagtccggg cagaccgagc
     6481 gaggccatcg ccgcgaagcc gaggatccag cccatcttcg gcatctgcag cagcaggccg
     6541 ccgaggcgct tgatctcgag cgtgtggtag cgctccttga tcgacccggc gacgaagaac
     6601 agcatgccgg tgatcagacc gtgggcgacc atgccgaaga tcgcggcgtt gaagccgagc
//
LOCUS       DAORNF010000079         6605 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_8636_length_6605_cov_1.697863, whole genome shotgun sequence.
ACCESSION   DAORNF010000079 DAORNF010000000
VERSION     DAORNF010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 6605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6605
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8636_length_6605_cov_1.697863"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(81..668)
                     /locus_tag="PLV68_04545"
     CDS             complement(81..668)
                     /locus_tag="PLV68_04545"
                     /inference="COORDINATES: protein motif:HMM:NF014664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HRE00544.1"
                     /translation="MSRHTRLRPGELDEAQQALYDSITGGRRAAGPQLFRLTEDDGSL
                     TGPFSALLLVPAIGRHVSRLGEAIRFESSLDGRTREIATLAVAAHWKSDFEQYAHEPL
                     ARRAGLDEQTIARIREPESCGALDDPADDAVHQLARRAVSATMDDATWENAVAVLGEA
                     AAVEVLVLVGYYTQLAIILDALAIGLPTNAAGPAR"
     gene            complement(720..>1804)
                     /locus_tag="PLV68_04550"
     CDS             complement(720..>1804)
                     /locus_tag="PLV68_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00545.1"
                     /translation="RHITITGRIVDTNGDPLTTELAPLDFLFWSYDVNDENLGGWASQ
                     VGWDENGIFQIEADINVIATSIELFIGGEDGEVPSVTGTFDPGADPTTVDVGDYEYPW
                     ADSVPVDISGVWDVYDLWGDLDSDLRPETLEVEVIPYAFDPSDVDEEIPYTRLAAPPA
                     KEVGVDHDSGGYGASFTLPHGTTLIDVRVTNPDNGVTYSAGYYLGEFDGHRVTLEHVD
                     FEDRPSHFTVEYTTFSGPAVLGDDDEPECMNLDAPRALLFRFGWAWWHAEEDVGAPEP
                     FGRRLIIPDPETGKATVTVGLPVDADLTEFVHTEDAPLDLDGDPIYAGIDRINDVGGS
                     GGLNFDFSEVVGASTGLDHRMLCHWP"
     assembly_gap    1805..1822
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1823..>2203)
                     /locus_tag="PLV68_04555"
     CDS             complement(<1823..>2203)
                     /locus_tag="PLV68_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00546.1"
                     /translation="GPSFDWFGDPVEFGDDATASAEVTRHHVSAIATTTARIRMGGVP
                     AVSLKITIKGYDGATYLGQVAKTFTTSDPAGVFGTDLVIGAFPNRSTRVEVEAQYRLS
                     GEDFDRGFGTGGDVLIDEDGVVSYH"
     assembly_gap    2204..2351
                     /estimated_length=148
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2352..>5266)
                     /locus_tag="PLV68_04560"
     CDS             complement(<2352..>5266)
                     /locus_tag="PLV68_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00547.1"
                     /translation="FLAQASAVLDSLASICNTSVDSHAAALDGLALELSIGRTPTDLL
                     QYSCQYVVTGLVSSDASPIVGVYLDVSAPGLTTCTTISSVTGSYQCVLTPDLDSIIAE
                     PATFPLPVTVAAHLTADGPAVATVGGEFAAKAAVGTRGSVIVDVDVDAALLPNIDVFG
                     TAFAKGDPAPPFYMELVGRNASGSTTATVNVKVTPNPVTAEYSVHRVFPAGTVSVKAT
                     ALIGVIAADRVDATAPSLGNGPNDLPFDIDYDPPVVVLTGTMTGVGGAPLAGTVPVTV
                     TAYHDSQWLGSWTATAEPTGPNGAYSTAPMTLPVATNQITAFAPIAVVQADYPTLTDH
                     PVHTGSQTVTFDVAYSPPTLAISGSMLRTGAPPNALAGARVLQARAYDASDVVVFNQA
                     VPVTVASDGAYSVVQSLPETAVRAEVYAIVGLTPADYASTTVPVPTLLANALTPVGLD
                     VTYNPPVLTVAGALKDQGGNPLGNTFVNVDAFAGATLLQSRQHLVSPGAGGAYTLTVD
                     LPLTTDNVSVTAKVGVVPGDWVSVNASDLHAGTNPTVPLDVTYGPPVARVSGTLTNGA
                     GNPLGATTIQVIAYNDNSPLFSATRTVTPAGGGSYTVDVDLPTSGWTRVVAIARIGSF
                     TSDYVTSSDADFGTGVIAVPLSAVYHPPIASFTGTLTGALGEPVTSTRVITVRAYDAA
                     DTILGQTTTAAIPNNMGVYPAALATLPRLATRITASVNPGTTSYDVVTEPYAVVPGTN
                     QLVLDAALNVPRLHLYGELTRDGAPLTSYINMYMAALDNNGNYLSYYQYFNGFQSDTG
                     SYDYFRTMPTGTVRVQGRIALVELGGFASDDILFDYPVSNGLNEFEIPIVQLSRSFHL
                     EGTVTRNGVPLANTTFPTYTWHKLNNTGTYSADARTIWITTDAVGHYVIDAALPMTAT
                     GIEMQYRVPSAFLGEGEIIVENTIDGFHAGGNTIDDFDVVATERVLTITGELSSDGV"
     assembly_gap    5267..5348
                     /estimated_length=82
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5349..5568)
                     /locus_tag="PLV68_04565"
     CDS             complement(<5349..5568)
                     /locus_tag="PLV68_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00548.1"
                     /translation="MSISLTVGNIINITVTADDDLPITGMLGTPNGKDPDTTDDFVPR
                     GGDVSVPVALVRQHSTDFYEFTDSWRVLD"
     assembly_gap    5581..5710
                     /estimated_length=130
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5711..>6605)
                     /locus_tag="PLV68_04570"
     CDS             complement(<5711..>6605)
                     /locus_tag="PLV68_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00549.1"
                     /translation="RMYFEPAAVTDFSSGTREVTLKATGRHTDDHWSITKKPARVDDV
                     LVDGEKVWHGSTTTPEEKLLTLVHNAGQDVCYDPVPETLEVTSQYRGVAKLDVTESDP
                     CYIKAEPAELTGTGTLTTHLLTRGFVVDTWAVDMSTVPPWLTLPTTSGELVKRTLNDP
                     EQSIALGFFAEVRQPTCTVQLPDRAEVVVNTTTRGTETIRITRPKVPRKETGCKKPKA
                     GASGDPHMESFDGVYYEGQVRGEYVLSVSDPGALPVDVEIRSRQEPTVGDGISRLADP
                     TSVTAVAVQAGVDLQMNPCGSR"
BASE COUNT          976 a         2008 c         2189 g         1054 t
ORIGIN      
        1 actacagaca gtagcgtaca cctgttcgta ttcgatcgca acccttcaac agagattctt
       61 ggtggctggc ggtcgcggtg ctaccgagcc ggtccagcgg cgttggtggg gaggccgatg
      121 gcaagggcgt cgaggatgat ggcgagttgg gtgtagtagc cgacgagcac gagcacctcg
      181 accgcggcgg cctcgcccag cacggccacg gcgttctccc acgtggcatc gtccatggtc
      241 gctgacacag cgcgacgggc gagctggtgg acggcgtcgt ctgctgggtc gtcaagtgca
      301 ccacaggact cgggctcgcg gatgcgagcg atcgtctgct catccagccc cgcgcggcga
      361 gcgagtggtt catgggcgta ctgctcgaag tccgacttcc agtgcgcggc gaccgccagc
      421 gtggcgatct ccctcgtccg gccgtcgagc gacgactcga aacggatcgc ctccccgagc
      481 cggctgacgt ggcgaccgat cgccggcacg aggagcagtg ccgagaacgg acctgtcaac
      541 gacccatcgt cttcggtcag gcggaacagc tgcggtcccg cggcgcgacg cccgccggtg
      601 atcgagtcgt acaacgcctg ctgagcctcg tcgagctcgc ccggccgcag tcgggtatgt
      661 cgtgacatcg caccacctcc cggtttcgtg gacgccacat gttggcggac ccgtgaccgt
      721 cagggccagt ggcagagcat gcggtggtcg aggccggtgc tggcaccgac gacctcactg
      781 aagtcgaagt tcaggcctcc actgccgccg acgtcgttga tgcgatcgat acccgcgtag
      841 atcgggtcgc catcgaggtc gagcggggca tcctcggtgt ggacgaactc ggtcaggtcg
      901 gcgtcgactg gcagacccac ggtgacggtc gccttgccgg tctccgggtc ggggatgatc
      961 agccgtcgcc cgaacggctc gggtgcgccg acgtcctcct cggcgtgcca ccacgcccag
     1021 ccgaaccgga acagcaaggc acggggcgcg tcgaggttca tgcactcggg ctcgtcgtcg
     1081 tcaccgagta cggcagggcc ggagaaggtc gtgtactcga ccgtgaagtg actcggccga
     1141 tcctcgaagt cgacgtgttc cagggtgacc cggtggccgt cgaattcgcc caggtagtag
     1201 cccgcgctgt acgtgacgcc gttgtcgggg ttcgtcaccc gcacgtcgat cagcgtggtg
     1261 ccgtgaggca gcgtgaacga cgccccgtat ccaccgctgt cgtgatcgac accgacctcc
     1321 ttggccggcg gcgcggcgag acgggtgtat ggaatctcct cgtccacgtc gctggggtcg
     1381 aacgcgtacg ggatgacctc gacctccaac gtctccgggc gcagatcgga gtcgaggtcg
     1441 ccccacaggt cgtacacgtc ccacacgccc gagatgtcga ccggcaccga gtcggcccac
     1501 gggtactcgt agtcgccgac gtcgaccgtg gtcgggtcgg cgccagggtc gaacgtgccg
     1561 gtcacactcg gcacctcgcc gtcctcgccg ccgatgaaca actcgatcga cgtggcgatc
     1621 acgttgatgt ccgcctcgat ctggaagatg ccgttctcgt cccagcccac ctggctggcc
     1681 cagccaccga ggttctcgtc gttcacgtcg taggaccaga acaagaagtc gagcggggcc
     1741 agttcggtcg tcagcgggtc accgttggta tcgacgatgc gacccgtgat cgtgatgtgg
     1801 cgggnnnnnn nnnnnnnnnn nngtggtacg acaccacacc gtcctcgtcg atcagcacat
     1861 cgccgccggt gccgaaacca cggtcgaagt cctcgccgct gaggcggtac tgcgcctcga
     1921 cctcgacccg cgtcgagcgg ttggggaagg cgccgatcac caggtcggta ccgaacacgc
     1981 cggccggatc gctggtggtg aacgtcttgg cgacctggcc caggtacgtg gcgccgtcgt
     2041 aacccttgat cgtgatcttc agcgacacgg cgggcacgcc acccatgcgg atgcgcgccg
     2101 tggtggtggc gatggcggag acgtggtggc gggtgacctc ggccgacgcc gtcgcgtcgt
     2161 cgccgaactc gaccgggtcg ccgaaccagt cgaagctcgg cccnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn ncgacaccgt cgctgctgag ctcgcccgtg atcgtcagca cccgttcggt
     2401 ggcgacgacg tcgaagtcgt cgatggtgtt gcccccggcg tggaagccgt cgatggtgtt
     2461 ctcgacgatg atctcgcctt cgcccaggaa cgccgacggg acgcggtact gcatctcgat
     2521 gccggtggcc gtcatcggca gcgcggcgtc gatgacgtag tggccgaccg cgtcggtggt
     2581 gatccagatg gtgcgcgcgt ccgcgctgta ggtgccggtg ttgttcagct tgtgccacgt
     2641 gtacgtcggg aacgtcgtgt tcgccagggg cacgccgttg cgcgtcaccg ttccttccag
     2701 atggaatgat cgcgacaact gtacgatcgg gatctcgaac tcgttgagtc cgttgctgac
     2761 cggatagtcg aacaggatgt catcgctggc gaacccgccc aactcgacca gcgcgatccg
     2821 gccctgcacc cgcacggtgc cggtcggcat cgtgcggaag tagtcgtagc tgcccgtgtc
     2881 ggactggaag ccgttgaagt actggtagta gctgagatag ttgccgttgt tgtccagcgc
     2941 ggccatgtac atgttgatgt acgacgtgag cggtgcgccg tcgcgggtga gctcgccgta
     3001 caagtgcaaa cggggcacgt tgagcgccgc gtcgagcacc aactgattgg tgccgggcac
     3061 caccgcgtag ggctcggtca ccacgtcgta gctggtggtg ccagggttga ccgatgcggt
     3121 gatccgggtc gccaagcgag gcagcgtcgc cagcgcggcc gggtacaccc ccatgttgtt
     3181 ggggatcgcg gcggtggtcg tctggcccag gatggtgtcg gccgcgtcgt aggcgcgcac
     3241 ggtgatgaca cgtgtcgacg tgaccggctc gcccagggca ccggtcagcg tgccggtgaa
     3301 cgacgcgatc ggcgggtggt agacggcgct gagcggcacc gcgatgaccc cggtgccgaa
     3361 gtcggcgtcc gaggacgtca cgtagtcgct cgtgaacgac ccgatccgcg cgatggccac
     3421 gacgcgggtc cacccgctgg tcggcaggtc gacgtcgacc gtgtacgacc caccaccggc
     3481 aggggtgacc gtccgcgtcg cgctgaacaa cggcgagttg tcgttgtacg cgatcacctg
     3541 gatggtcgtc gcaccgagcg gattgccggc accgttggtc aacgtgccgc tgacccgtgc
     3601 gaccggcgga ccgtaggtga cgtcgagcgg aacggtcggg ttggttccgg cgtgcaggtc
     3661 ggacgcgttc acgctgaccc aatcgcccgg caccacaccg accttggcgg tgaccgacac
     3721 gttgtcggtg gtcaacggga ggtcgacggt cagcgtgtag gcaccacccg caccgggcga
     3781 caccagatgc tgacgggact gcagcagcgt ggcgccggcg aaagcgtcga cgttgacgaa
     3841 cgtgttgccc agcgggttac cgccctgatc cttcagcgca ccggccacgg tgagcaccgg
     3901 cgggttgtag gtgacgtcga gccccaccgg agtgagcgcg ttggccaaca gcgttggcac
     3961 cggcaccgtg gtcgaggcgt agtccgcggg ggtgaggccg acgatggcgt agacctcggc
     4021 ccgcaccgcc gtctcgggca acgactgcac cacggagtac gcgccgtcgc tcgcgacggt
     4081 caccgggacg gcctggttga acaccaccac gtccgacgcg tcgtatgcgc gcgcctgcag
     4141 cacccgggcg ccagcgagcg cgttgggcgg tgctcccgta cgcagcatcg aaccgctgat
     4201 cgccaacgtc ggcgggctgt aggcgacgtc gaaggtgacg gtctggctgc ccgtgtgcac
     4261 cgggtgatcg gtcagcgtgg ggtagtcggc ctgcaccacc gcgatcgggg cgaacgcggt
     4321 gatctggttg gtcgcgacgg gcagcgtcat cggagccgtc gagtatgcgc cgttcggccc
     4381 ggttggctcg gcggttgccg tccaggaacc gagccactgg ctgtcgtgat aggcagtgac
     4441 ggtcaccggc accgtcccgg cgaggggcgc cccgccgact ccggtcatcg tgcccgtcag
     4501 caccacgacc ggcgggtcgt agtcgatgtc gaacggcaag tcgttcggcc cgttgcccag
     4561 gctgggcgcg gtggcgtcga cccggtcggc ggcgatcaca ccgatcaacg ccgtcgcctt
     4621 gaccgacacc gtgcctgccg ggaacacccg gtgcacggag tattcagcgg tcaccgggtt
     4681 gggcgtcacc ttgacgttga cggtcgcggt ggtcgaacca ctggcgttgc ggccgaccag
     4741 ttccatgtag aagggtggcg ccggatcacc cttggcgaac gccgtcccga acacgtcgat
     4801 gttggggagc aacgccgcgt cgacgtcgac gtcgacgatg accgaaccgc gcgtccccac
     4861 cgcagccttc gccgcgaact cgccaccgac tgtcgccacc gccgggccgt cagccgtcaa
     4921 gtgagcggcg acggtgacgg gcagcgggaa cgtggccggt tcggcgatga tcgagtcgag
     4981 gtcgggcgtc agcacgcact ggtacgagcc ggtgacgctg ctgatggtgg tgcaggtcgt
     5041 cagaccgggg gcactgacgt cgagatacac gcccacgatc ggtgacgcgt cggacgacac
     5101 cagaccggtg accacgtact ggcacgagta ctgcagcaga tcggtcggcg tgcgcccgat
     5161 cgacagttcc agcgccaggc catcgagggc tgcggcgtgc gagtccaccg acgtgttgca
     5221 gatggacgcg aggctgtcca gcaccgcgct ggcctgggcg aggaacnnnn nnnnnnnnnn
     5281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5341 nnnnnnnncg tcgagcactc gccacgagtc ggtgaactcg tagaagtcgg tcgagtgttg
     5401 acgcaccagc gcgaccggca ccgagacgtc gccgccgcgg gggacgaagt cgtcggtcgt
     5461 atcagggtcc ttgccgttgg gggtgccgag catgccggtg atcggcaagt cgtcgtcggc
     5521 cgtgaccgtg atgttgatga tgttgccgac cgtgagcgag atgctcaacc ccggcgcggt
     5581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5701 nnnnnnnnnn cgactcccgc acgggttcat ctgaaggtcg acaccggcct gcaccgcaac
     5761 ggcggtgacg ctggtcggat cggccagacg ggagatgcca tcgcccaccg tcggctcctg
     5821 acgagaacga atctcgacgt cgaccggcag cgcacccggg tcgctgaccg acaggacgta
     5881 ttcgccgcgg acctggccct cgtagtacac gccgtcgaac gactccatgt gcgggtcacc
     5941 cgaggcgccg gccttcggct tcttgcagcc ggtctccttg cgcggcacct tcggccgggt
     6001 gatgcgaatg gtctccgtgc cgcgagtcgt ggtgttgacg accacctcgg ccctgtcggg
     6061 cagctgcacg gtgcaggtcg gttggcgcac ctcggcaaag aatccgaggg cgatcgactg
     6121 ctccgggtcg ttgagggtgc gcttgaccag ttcgccgctg gtcgtcggca gggtcaacca
     6181 ggggggcacc gtgctcatgt cgacggccca cgtgtcgacc acgaagcctc tggtcaacag
     6241 gtgggtggtc agcgtgccgg tacccgtcag ttccgcaggt tcggccttga tgtaacacgg
     6301 gtcggattcg gtgacgtcga gcttggcgac gccgcggtac tgcgacgtga cctccagcgt
     6361 ctcgggcacc gggtcgtagc acacgtcctg accggcgttg tgcaccaggg tcagcagctt
     6421 ttcctccggt gtggtcgtgc tgccgtgcca caccttttca ccgtcgacca acacgtcgtc
     6481 gacgcgggca ggcttcttgg tgatcgacca atggtcgtcg gtgtgacgtc cggtcgcttt
     6541 cagtgtgacc tcgcgcgtgc cggagctgaa gtcggtgacg gcggctggtt cgaagtacat
     6601 ccgtc
//
LOCUS       DAORNF010000080         6591 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_8658_length_6591_cov_1.966340, whole genome shotgun sequence.
ACCESSION   DAORNF010000080 DAORNF010000000
VERSION     DAORNF010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 6591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6591
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8658_length_6591_cov_1.966340"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            45..683
                     /locus_tag="PLV68_04575"
     CDS             45..683
                     /locus_tag="PLV68_04575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HRE00550.1"
                     /translation="MRIIAFHKPFHVLTQFTDSEGRATLRSFIDIPGIYAAGRLDYDS
                     EGLLMLTDDGPLNQNLTDPQHERPKIYWAQVEGKATPAQLAPFESGIVLSGERMRPAK
                     ARIIADPRLGVRQSYADPAHPVPAPVRDYHPTTWLEIEVHEGKKHQVRRMTAAVGLPC
                     LRLVRYAIGPITLDELACGAWRPLTEAEIYLLEAQARHSPARRAAKKAQRHG"
     gene            676..>1316
                     /locus_tag="PLV68_04580"
     CDS             676..>1316
                     /locus_tag="PLV68_04580"
                     /inference="COORDINATES: protein motif:HMM:TIGR01902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRE00551.1"
                     /translation="MDEIALLTGLLERYSPTGEEAAAVGYLVEAMQAAGLAAHIDEIG
                     NAIGVVGDGPRQIVLLGHIDTVAGFIPVTRDGDRLNGRGAVDAKGPLATFAAAAARCV
                     GHDLGDRQIVVIGAVGEEGNSRGAHHIKDRYRPAATVIGEPSSWAKVTLGYKGSAWFD
                     LKAARALAHTSAQAESACEAAVAGWNAVAGWAATYNADRPRMFDQVLPTLRRM"
     assembly_gap    1317..1440
                     /estimated_length=124
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1441..1717
                     /locus_tag="PLV68_04585"
     CDS             <1441..1717
                     /locus_tag="PLV68_04585"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRE00552.1"
                     /translation="TLFEGVDAYRGDKNTPLVRAFLAAIRKQGGQPSFSVKSGTSDMN
                     IVAPLWGTPILAYGPGDSSLDHTPHEHIFLTEYQKAIDVLTTVLLTI"
     assembly_gap    2589..2709
                     /estimated_length=121
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2710..3188
                     /locus_tag="PLV68_04590"
     CDS             <2710..3188
                     /locus_tag="PLV68_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00553.1"
                     /translation="AGIRLSDGTTLNYSGDDVYWTLRDAEFVTGLAPEARMPIEMTVA
                     NLPDGVTITDIDWQIQSNPTDDIGRTDISYTVESTSERNGQYCVAYNVVIPDPAITSG
                     VFVSLWLVDDAGKVIGVGETYENAEFAPGGRSYRKTICASVLSGTVATHRVAIWGR"
     assembly_gap    3209..3251
                     /estimated_length=43
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3322..>3595
                     /locus_tag="PLV68_04595"
     CDS             3322..>3595
                     /locus_tag="PLV68_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00554.1"
                     /translation="MKKSRLIVTASLVSSLVLGISPAFAQGTGPQRTAPESLQELKIQ
                     DTTGLVGVERLSPELASATGPVKVVVRLAGQPVAYTYAAGAEAAEQV"
     assembly_gap    3596..3605
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3606..4755
                     /locus_tag="PLV68_04600"
     CDS             <3606..4755
                     /locus_tag="PLV68_04600"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRE00555.1"
                     /translation="AAAQADVIAAAQALDSSAAVLGQTRNALNAVILRMEASQLAALS
                     QNPGVLSIHPVRDYTMDLGETVPYIGATAALRAEGVDGSGVLVAVIDSGVDYTHIKLG
                     GDGLLSTYARAYCGDPAAIPNPTDPACTAAKLVADPALFPNSKVVGGWDFVGETWPLT
                     AEQPDPNPIDFDGHGSHVADIIAGLEGGGVGEGVAPGAEILAIRACSAVSTSCSGIAL
                     LQAVDYALDPNGDGDLDDRAQIINMSLGSNYGTAYNDDLSHAVDGATVANTLVVASAG
                     NGGNRPYIAGTPASAWTALSVAQTQVPGATQYALRITAPASIAGLFTATATVEWAPIT
                     TGFSGDIAYVGRACPADSIAAAVSQAAVNRLDRPRPTSTCISLLPASR"
     gene            complement(4814..6250)
                     /locus_tag="PLV68_04605"
     CDS             complement(4814..6250)
                     /locus_tag="PLV68_04605"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRE00556.1"
                     /translation="MGAQRHQESPHGVVHHSVVRHDGIRRERVRREPRRRRAAALSVM
                     VAAALTACSSSGQPSSSGANSTTTVGSTAPAASSTEASTVGSSAVEITQVVRVGYAYP
                     DLKAFSTLNKELGIGDPELQAEAVVDGWRRDGLLPPGMDVEVVYAPYNILSNSEKLGA
                     CTTLAQDEDVFAVVSGLTFTTGAECLATRFGIPVIDTEGAPRSLYERGAPWLFTLRAD
                     QVTQFGNYGRWLVDSGVLEGKRVGLYFETPLTEGVDAMRAILKEAGIDIVSTTESTGV
                     GIGSTEDEVSVRRFIEDKVDVVLPLVGGSSAVNMYNFAEAQDFHPEYYDLDTAEHTTD
                     VAARTNPTSQYDGTTAMTMTRIGELAGGVDNSGREACMANYERYAAQDLSREPPESGE
                     LSSILRTCDLFAVLLAGLHGAAADLTRENFVTALENSGEIELVGSANGSFSATDHSLI
                     DEYRSIQWDAACPCWRAISDFQKVSVID"
     gene            6332..>6591
                     /locus_tag="PLV68_04610"
     CDS             6332..>6591
                     /locus_tag="PLV68_04610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00557.1"
                     /translation="MAYAGRSGSATWTKEADVEPRTATDNRHDSTDIVVAPLTGSVGV
                     EVTGVDLDEPEVFTPRLGGLERKGAIGLVGLSEPEAMRHYVRL"
BASE COUNT         1103 a         2133 c         2006 g         1051 t
ORIGIN      
        1 cgtgcgatgg gcgtaccgct tgcggtatga tgcgtcacac aaccatgcgc atcatcgcct
       61 tccacaaacc ctttcacgtg ctgacccaat tcaccgattc ggagggacgc gccacgctca
      121 ggtccttcat cgacatcccg ggcatctatg cggccggccg tcttgactat gacagcgagg
      181 ggctgcttat gctgaccgat gacggtccgc tcaaccaaaa cctcaccgat ccacaacacg
      241 agcggccaaa gatctattgg gcccaggtcg aaggtaaggc cacgccggcc caactcgcgc
      301 cgttcgagag cggcatcgtg ttgagtgggg agcggatgcg cccggcgaag gcgcgcatca
      361 tcgccgaccc gcggcttggc gtccggcaga gctacgccga cccggcccac cctgtgccgg
      421 ctccggtgcg cgattatcac ccgacgacct ggctcgagat cgaggtccac gagggcaaga
      481 agcatcaggt gcgccggatg acggccgccg tggggctccc atgtttgcgg cttgtacgtt
      541 acgcgatcgg gccgatcacg cttgacgaac tggcctgcgg tgcctggcga cccttgaccg
      601 aagccgagat ctatctgctt gaagctcagg cgcgtcactc gcctgcccgc cgggcggcga
      661 agaaggcgca acgccatgga tgagatcgcg ctgttgacgg ggttgctcga gcggtacagc
      721 ccgactggcg aagaagccgc ggccgttggc tatctggtcg aggcgatgca ggccgccggg
      781 ctcgccgcgc acatcgacga gatcggcaac gcgatcggcg tggtgggcga cggcccgcga
      841 cagatcgtgc ttcttgggca catcgacacg gtggccgggt tcatccccgt cacgcgcgac
      901 ggcgatcgcc tgaacgggcg tggcgccgtg gatgccaaag ggccgctggc gacctttgct
      961 gcggccgcgg cgcgctgcgt ggggcacgac cttggcgacc ggcagatcgt ggtgatcggc
     1021 gcggtcggcg aagagggcaa cagccggggc gcgcatcaca tcaaagaccg ctaccgaccg
     1081 gccgcgaccg tgatcggcga gcccagcagt tgggccaagg tgacgctggg ctacaagggc
     1141 agcgcgtggt tcgatctcaa agccgcgcgg gcgctggcgc acacgtcggc ccaggccgag
     1201 agcgcctgcg aagccgcggt ggccggctgg aacgcggtgg ccggctgggc cgcgacttac
     1261 aatgccgatc gaccgcggat gttcgatcag gtcttgccga ctctcagacg gatggannnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 gacgttattc gagggcgtcg acgcctatcg tggcgacaag aatacgccac ttgtgcgggc
     1501 gttcctggcc gcgatccgca agcaaggcgg ccagccgtcg ttttctgtca agagcggcac
     1561 aagcgacatg aatatcgtcg ccccgctctg ggggacaccg attctggcct acggcccggg
     1621 cgactcgagc cttgaccata cgccgcatga gcacatcttc ctgaccgagt atcaaaaggc
     1681 gattgatgtg ctgacgacgg tcctgctgac gatctagcga tgctcgcgca ggtgtcaggc
     1741 tcacataaat gcattggtcg agagcctctc aaccattttg cggaggccgg tccggcggta
     1801 gacggtacgc tggtaccact gttcagggtt tgatgtcccc gtttcttgac atgggcagag
     1861 cgcctgacta tcatcaagct atccggtatt gtgacacaaa tccaatacga taggtcacgc
     1921 accactcgcc tgttgagccc aaacacgcaa ggggttcgca ggtgtggcgc ggcctcggtg
     1981 gtatcaccga acggcagatc gaccacggcg ctcgccctgt gtcaacgact actcacccgt
     2041 tgcccgtagc gaccaaacgt cgccgcccac gacgcgacca gcgtctcaac cgctgggtcg
     2101 tcgtcgccgg tctggcgacg atgcttgcgc cgctatgcgc cttgatcctg tttggcgcga
     2161 tcctctatcg cgatcgcgcc ttttcgggct ggatcatggg cccggccgcg caactgggtt
     2221 cagacaccgc ggcccctgag ggttcgagcg cgggtgtact tctgccgacg gccacaccat
     2281 cggccctgat cagcgatgcg atcgccgatt tcgcgacggc gactgtgggg tcttatccgg
     2341 cgctgatcga agccacgtcg acgcaacccc tggttgccgg ccccgcagcg accgccacgt
     2401 cgcagccccc gtctgcgcca acggccgtga tggccagccc caacgccccg gcgccaaccg
     2461 cgaccgcgaa cctcgacgcc acgccgatcc ctccgtcgcc cacagcgctt atcaatggca
     2521 cgccgacggc tgcgccgccg actgcaactt ccggtcccac gcagcctgtg gctacggcga
     2581 cgagcccgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnc ggccgggata cggctcagcg acggcacgac gctcaactac tcgggcgacg
     2761 acgtttattg gacgttgcgg gatgccgaat tcgtgacggg cctggcgccg gaggcgcgca
     2821 tgccgatcga gatgaccgtc gccaacctgc ccgacggtgt gacgatcacg gacatcgact
     2881 ggcagatcca atcgaacccg accgacgaca tcggtcgaac cgacatctcc tacaccgtcg
     2941 aatcgacgag cgaacgaaac ggccaatatt gcgtcgccta caacgtcgtg atcccggacc
     3001 ccgcgatcac gtcgggcgtc tttgtcagtt tgtggcttgt cgacgacgcc ggaaaagtca
     3061 tcggcgtcgg cgagacctat gagaacgcgg aatttgcgcc gggtgggcgt tcctatcgca
     3121 agaccatctg cgcgtcagtg ctcagcggga cagtggccac gcaccgcgtg gcgatctggg
     3181 gccggtgagt ggtgcaatcg ttcgatcgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn ngtgacccgg tgcgaattgc caaacacgtg cgcctgaacg tcagtaacac
     3301 tgaaaagcag gaagaaaact catgaagaaa tctcgtttga ttgtgaccgc atcgttggtg
     3361 tcgagccttg tgctcgggat cagcccggcc tttgcgcagg gcacaggccc gcagcgcaca
     3421 gcgcccgaat cgctccagga actcaagatc caggacacca caggtctcgt gggcgtcgag
     3481 cggctcagcc ctgagttggc tagcgcgaca gggccagtga aggttgtcgt gcgtctggcc
     3541 ggtcagcccg tggcctacac ctacgcggcc ggcgcagaag ccgccgagca ggtggnnnnn
     3601 nnnnncgcgg cggcccaggc cgacgtgatc gccgcggcgc aggcgcttga ctcaagcgcg
     3661 gccgtgctcg gccagacccg caacgccctg aacgccgtca tcctgcggat ggaggccagc
     3721 cagctggcag cactctcgca gaaccctggc gtgctctcga tccatccggt ccgcgactac
     3781 acgatggatc tgggcgagac ggtgccatac atcggcgcga cggcggctct gcgcgccgaa
     3841 ggtgtcgatg gcagcggcgt gctcgtcgcc gtgatcgact cgggcgttga ctacacacac
     3901 atcaagctcg gtggcgatgg cttgctcagc acgtatgccc gcgcgtactg cggcgacccg
     3961 gcggccatcc ctaatccgac tgacccggcc tgcacggccg cgaagctggt tgccgacccc
     4021 gcgctgttcc cgaacagcaa ggtcgtgggt ggctgggact tcgtcggcga gacctggccg
     4081 ctgactgccg agcagcccga cccgaacccg atcgacttcg acggccacgg ctcgcatgtc
     4141 gccgacatca tcgccggcct cgagggcggc ggcgtcggtg aaggcgttgc gccgggcgcc
     4201 gagatcctgg cgatccgcgc ctgctcggcc gtgtcgacca gctgcagcgg catcgccctg
     4261 ctccaggccg tcgactatgc gctcgacccg aacggtgacg gcgacctcga cgaccgcgct
     4321 cagatcatca acatgtcgtt gggctcgaac tacggcacag cctacaacga cgacctgtcg
     4381 cacgccgtcg acggcgcgac cgtggccaac accctggtcg tggcctcggc cggcaacggc
     4441 ggcaaccgcc cgtacatcgc tggcacgccc gcgagcgcct ggacggccct ctcggttgcc
     4501 cagacccagg tgcccggcgc cacgcaatac gcgctgcgca tcacggcccc ggccagcatc
     4561 gccggcctgt tcacggcgac ggcgaccgtc gagtgggcgc ccatcacgac cggcttctcg
     4621 ggcgacatcg cctatgtcgg gcgcgcctgc ccggcggatt cgatcgcggc tgcagtatca
     4681 caggcagccg tcaaccggct cgaccgcccg agaccgacgt ctacctgcat ctcactgctc
     4741 cctgcatctc gctaaacccc cttggccagt tcagtatact cttttacctc ctagtcggtc
     4801 tcccgacgac ggttcagtcg atgaccgaca ccttctggaa gtcggagatg gctcgccaac
     4861 acgggcacgc tgcgtcccat tggatcgacc ggtactcgtc gatcaacgaa tgatcggtag
     4921 cggagaagga gccgttggca ctgcccacga gttcgatctc gccgctgttc tcgagcgctg
     4981 tcacgaagtt ctcacgggtg aggtcggccg cagcaccgtg cagtccggcg agcaacaccg
     5041 cgaagagatc gcaggtgcgc agaatgctgc tcagctcgcc cgactccggt ggctcacgag
     5101 acaggtcctg ggccgcgtag cgctcgtagt tcgccatgca tgcctcgcgc ccgctgttgt
     5161 cgacgccacc cgcgagctcg ccgatccggg tcatcgtcat ggccgtcgtg ccgtcgtact
     5221 gcgacgtcgg attggtgcgc gcggccacgt cggttgtgtg ttcggcggtg tcgaggtcgt
     5281 agtactccgg atggaagtcc tgtgcttcgg cgaagttgta catgttgacc gcgctactcc
     5341 caccgaccag cggcagcacc acgtcgacct tgtcctcgat gaaccgtcgc accgacacct
     5401 cgtcctcggt gctcccgatc cccacgcccg tcgactcggt cgtcgacacg atgtcgatgc
     5461 cggcctcttt caggatggcg cgcatcgcat cgacaccttc ggtgagcggc gtctcgaagt
     5521 acagcccgac ccgtttgccc tcgagcacac ccgagtcgac cagccatcgc ccgtagtttc
     5581 cgaactgcgt cacctgatcg gcgcgcagcg tgaacagcca cggcgctccg cgctcgtaca
     5641 acgacctcgg tgcgccctcg gtgtcgatca ctgggatgcc gaatcgcgtc gccaggcact
     5701 cggcgccggt ggtaaaggtc aagccgctga cgacggcgaa cacgtcttcg tcctgagcca
     5761 gcgtcgtaca ggcgccgagc ttttcggagt tcgacaggat gttgtacggg gcgtagacga
     5821 cctcgacgtc catccccggc ggtaacagac cgtcacgccg ccagccgtcg acgaccgcct
     5881 ccgcctgaag ctcgggatcg ccgatgccga gttccttgtt gagcgtggag aaggctttga
     5941 gatcgggata cgcataaccc actcggacga cctgggtgat ctcgacagcc gaggagccga
     6001 ccgtgctcgc ttcggtgctg ctggccgcgg gggccgtcga cccgacagtg gtcgtcgagt
     6061 tggctcccga cgacgatggt tggccagacg acgaacacgc ggtcaacgcc gcggccacca
     6121 tcaccgacaa cgcagcagca cgacgccgcc gtggctcgcg acgaacccgc tcgcgacgaa
     6181 tcccgtcgtg acgaaccaca ctgtgatgaa ccacaccgtg cggcgactcc tgatgacgct
     6241 gtgcgcccac ctgattcccc tttcggccac cgtcgagcga agagctccac gatgcagcaa
     6301 cgttgagtag agtacactga atcggggatc gatggcatac gccggcaggt cgggctccgc
     6361 gacatggacg aaggaggctg acgtggaacc acgcaccgca accgacaatc ggcacgactc
     6421 gaccgacatc gtcgtcgccc cgctgaccgg ctcggtggga gtcgaagtca ccggtgtcga
     6481 tctcgacgag ccggaggtct tcacgccgcg tctcggcggg ctggagcgca aaggcgccat
     6541 cggcctcgtc ggcctttcgg agccggaggc gatgcgccat tacgtgcgcc t
//
LOCUS       DAORNF010000081         6582 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_8673_length_6582_cov_1.840815, whole genome shotgun sequence.
ACCESSION   DAORNF010000081 DAORNF010000000
VERSION     DAORNF010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 6582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6582
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8673_length_6582_cov_1.840815"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /locus_tag="PLV68_04615"
     CDS             <1..353
                     /locus_tag="PLV68_04615"
                     /inference="COORDINATES: protein motif:HMM:NF013916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00558.1"
                     /translation="SGVHVKAILETGELPDQHTVRIAAEAAIAGGAHFVKTSTGKTPV
                     SATLEAAETMLHVISQADREVGLKPSGGIRTVGDAARYLDLADEIMGADWATPETFRF
                     GASGLLTDVVTHLS"
     gene            complement(360..824)
                     /locus_tag="PLV68_04620"
     CDS             complement(360..824)
                     /locus_tag="PLV68_04620"
                     /inference="COORDINATES: protein motif:HMM:NF025939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HRE00559.1"
                     /translation="MADWEHRLEELGIQLRDPLPAGGLYTSVVVVDGFAYTSGTVGVD
                     GPPWRLAHPGCLGDDLDLETGQSSARKAMISLLGNLRGGIGTLDRVERFVKITGFVRA
                     VPAFTDVPKVMDGASQLLVDIFGQPCARSAIGVSALPGGASVEIDSVVKLAW"
     gene            971..1657
                     /locus_tag="PLV68_04625"
     CDS             971..1657
                     /locus_tag="PLV68_04625"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HRE00560.1"
                     /translation="MGETLAERIENEIIASGWKVGDVIGSESDLRARYGVSRAVFREA
                     IRIVGHHGVAEMRRGPGGGLVVMEPRIDTGARTVSLNLDYLQITPRQINEARSAIEIA
                     CVHMVAQNLTAAGADRIRAFLAEEVDMIVSQGSLGEPRDDFPSNHFHVLLAELTGNPA
                     MMLFVQILSQVTSRHSVLVGSAEELHTNAEQVHKVHSKIAEAILKKDVEGAKRRMRRH
                     LVALVDYLHD"
     gene            complement(1839..>2315)
                     /locus_tag="PLV68_04630"
     CDS             complement(1839..>2315)
                     /locus_tag="PLV68_04630"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRE00561.1"
                     /translation="LVNNAGILRDRMIANMSEDEFDSVVRVHLRGHFCPLRHAAEYWR
                     SQAKAGVVCDRSVVNTSSTSGLLGNVGQGNYGSAKAGIASMTIIADLELRRYGVRVNA
                     IARRGPGSPPGWHRLLIPTRSTPGILPMCRRWWRISPPRGARCTVRFSSFTAGESN"
     assembly_gap    2316..2433
                     /estimated_length=118
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2434..2625)
                     /locus_tag="PLV68_04635"
     CDS             complement(<2434..2625)
                     /locus_tag="PLV68_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRE00562.1"
                     /translation="MVTSVGSELDGRVVIVTGAGRGLGRDHALLMAAEGARVVVNDLG
                     GSSDGAGADAGPAAETAAEI"
     assembly_gap    2740..2779
                     /estimated_length=40
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2780..4253
                     /locus_tag="PLV68_04640"
     CDS             <2780..4253
                     /locus_tag="PLV68_04640"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HRE00563.1"
                     /translation="ELERRSVQLARVWRRAGLRAGDGVAILAENTPWFHEIYWAAIRS
                     GLYLTPVNRHLSAPEVAYIVRDCGARAIVASANLVVVADAALHDNSVCAHRLVVGPAT
                     SDFLSYDTALAGESDEPFEDQPRGALMCYSSGTTGQPKGIRRALTGKQIDDPTTAASI
                     DLFVDVFGIDASTRYLSPAPLYHAAPLAWTAAVQALGGTTVIMETFDARRALVALHDH
                     RITHSQWVPTMFVRMLKLPAADRLGLDLSHHRCAIHAAAPCPPEVKRQMVDWWGPILW
                     EFYAGSEGNGATIISSEEWLSRPGSVGRPLFGVVHVCDDDGTEVAVGETGLVYFELPG
                     PAFVYHGDPDRTRDTRNPLHDRWTTIGDIGHVDHEGYLYLTDRRAFTIISGGVNIYPR
                     EIEDCLIMHPSIADVAVFGIPDADMGEAVHASIELARDVTPTADLDAALIAFARQRLA
                     GFKVPRSIDYVKSLPRMPTGKLDKQPLRERYWRQAPEVAG"
     gene            4250..>4839
                     /locus_tag="PLV68_04645"
     CDS             4250..>4839
                     /locus_tag="PLV68_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00564.1"
                     /translation="MSTGTGALVDRVGVRLDADVSGHARLPLSAAIERNGHVLASPLM
                     LAADIASGIRLHRPDVESVLTASFSFRRTDRRPLGAVTAAATRLWEHGRRVIDDVEYL
                     DEAGEQVGFGRIAFTVLGRRDGGSDADGSDAAAFSRFMTPRVDPIDTPLIDAAGIELV
                     DAAVGHARFRPEQDVGDRSVGDRGVAPHVVELRVAV"
     assembly_gap    4840..4849
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4850..5115
                     /locus_tag="PLV68_04650"
     CDS             <4850..5115
                     /locus_tag="PLV68_04650"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HRE00565.1"
                     /translation="SGADIVQGSFVTLLGEAAALAFAEHELAAAVTVRSLEVGYLAPV
                     AADTVTTRARWLGRRGAADIDVVACDVETGRVCATFVVGVTTV"
     assembly_gap    5141..5183
                     /estimated_length=43
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5184..6298)
                     /locus_tag="PLV68_04655"
     CDS             complement(<5184..6298)
                     /locus_tag="PLV68_04655"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRE00566.1"
                     /translation="MVVQHDFDLISVDDHIIEPPGVWTDRLPSKYQSAGPHVITKADG
                     TEAWEFEGEAIATMGLNAVAGKEHKDFAMDPTSYADMIPGCYDPIQRARDLLNDGVRG
                     SLCFPTFPRFAGQRFLTAKDKDLADLCVKAYNDWMLEEWCAAVPGLYIPMMIGQLWDP
                     QLMAAEIRRCAALGVRAVSFPESTAPFDQPSFHTEHWDPVWQAAVETDTVICIHIGTS
                     GRTHLPSPDAPFSVAVALGPVNAQITFMDLLLSGIPQRFPDIKIALSEGGIGWVPFAL
                     ERIDRTWERHRYWAKLDDTPPSELFRRNFWVCFIDEYAGLELRDHIGVDRIMWECDYP
                     HADTPWPHSQGEVRKGLADLPSDEVAAMTHGNAEKLFR"
BASE COUNT         1100 a         2142 c         2044 g         1085 t
ORIGIN      
        1 cgtcaggcgt gcacgtcaag gcgatcctcg aaaccggcga gctccccgac cagcacacgg
       61 ttcggatcgc ggccgaagca gccatcgccg ggggcgcgca cttcgtcaag acctccacgg
      121 gcaagacgcc cgtctcggcc accctcgagg ccgccgagac gatgctgcac gtcatcagcc
      181 aggccgaccg cgaggtgggg ctcaagccct ccggcggcat ccgcacggtc ggcgacgcgg
      241 cccgctacct cgaccttgcc gacgagatca tgggcgccga ttgggccacg cccgagacgt
      301 tccgcttcgg cgccagcggc ctgctcaccg acgtcgtgac ccacctcagc tgactcgcgt
      361 caccaggcca gcttgaccac gctgtcgatc tcgacgctcg ccccgcccgg cagcgcgctc
      421 acgccgatcg ctgatcgggc acatggttga ccgaagatgt ccaccaacaa ctgcgatgca
      481 ccatccatca ccttgggtac atcggtaaag gccggaaccg cccggacaaa accggtgatc
      541 ttgacgaaac gctcgacacg gtccagcgtc ccgatcccgc cgcgaaggtt gccgagcagg
      601 ctgatcatcg ccttgcgcgc cgacgactga ccggtctcca agtcgagatc gtcgccgaga
      661 cagcccggat gagcgagacg ccacggtgga ccatcgacgc caacggtccc cgacgtgtag
      721 gcgaagccgt cgacgacgac gacagaggtg tagagccctc ccgcgggcag gggatcacgg
      781 agttggattc ccagttcctc gagacggtgt tcccagtcgg ccacgtgttc tccctcgtcc
      841 tcatcggttg ggtctgacgc taaatgagta tactccattc cggcgggtcg tcgagtcacc
      901 actgtggcta cgctgaccaa ttgtgaatac tgaattgcta gcgaccccgc gcccaatcca
      961 aggtcgcagg atgggtgaaa cactggccga gcgcatcgaa aacgagatca tcgcatcggg
     1021 ttggaaggtc ggcgacgtga tcggttccga gtccgatctg cgcgctcgct acggagtcag
     1081 tcgagcggta tttcgcgaag cgatccgcat cgtcggccat cacggggtgg ccgagatgcg
     1141 gcgcggtccg ggtggcgggc tcgtggtgat ggagccgcgg atcgacacgg gggccagaac
     1201 ggtgtcgttg aacctcgact acctccagat cacaccgcgg cagatcaacg aggcacgttc
     1261 ggcgatcgag atcgcatgtg tgcacatggt cgcccagaac ctcactgcgg ctggcgctga
     1321 tcgcattcgg gccttcctcg ccgaagaggt ggacatgatc gtgagccagg ggtcgctcgg
     1381 agaaccgagg gacgactttc cctcgaacca cttccatgtg ctgctggccg agctgaccgg
     1441 caacccggcg atgatgctct tcgtccagat cctcagtcag gtgacgagtc ggcactcggt
     1501 gctggtcgga tcagccgagg aactgcacac caacgcggag caggtccaca aggtgcactc
     1561 gaagatcgcc gaggccatcc tcaagaagga cgtcgaaggg gccaagcgac gcatgcggcg
     1621 tcacctcgtc gccctcgtcg actacctcca cgattgaccg acggcacgat tgaccgacag
     1681 cacgactgac caaccggcac gactgagcct ccgttacgag ctcaaggcct cgatcgtcgg
     1741 aaacgacgtg gtcgcgaatc cgcgtgtcgc ctctgccagc tcggcgatgg tccagcgacg
     1801 atcggctcga atctcgtgat gcaacgtcca gggctgcatc agttggattc gcccgccgtg
     1861 aacgaagaaa acctgaccgt gcagcgggca cctcgaggtg gcgagatacg ccaccatcgg
     1921 cgacacattg gcaggatccc cggcgtcgaa cgcgttggga tcaggagtcg atgccacccc
     1981 ggcggtgagc ctggtccgcg ccgagcaatg gcgttgaccc tcacgccgta gcggcgcagc
     2041 tcgaggtcgg cgatgatcgt catcgaggcg atccctgcct tcgctgagcc gtagttgcct
     2101 tgtccgacgt tgccgagcag ccccgacgtc gatgaggtgt tgaccaccga ccgatcgcac
     2161 acgacgccgg ctttggcctg cgaccgccag tattccgctg catggcgaag cgggcagaag
     2221 tgcccgcgca gatgaacccg gacgacggag tcgaactcgt cctcgctcat gttggcgatc
     2281 attcgatcgc gcaagatgcc ggcattgttc accagnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngatctcg gccgcagtct cggcggcagg
     2461 accggcgtcg gcaccggcgc catcggacga gccgccaagg tcgttgacga cgacgcgagc
     2521 tccctcggcc gccatcagca gcgcatggtc ccgtcccagc ccacggccgg cacccgtcac
     2581 gatgaccacc cgtccgtcga gttctgagcc gaccgatgtc accatgagta ctccccttca
     2641 tcgcccgatc tggcctgcac caggcgtcgg ccaaccatcc gataaatcag tatactgaaa
     2701 gagatgtatc ccggggcgca tcctgctgac agaccggcgn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnc gaactcgaac gacgatcggt gcagctggcc agggtgtggc
     2821 gccgcgctgg cctgcgggca ggtgacggcg tggcgattct cgccgagaac acaccgtggt
     2881 tccacgagat ctactgggcg gcaatccgct ccgggctgta cctcacgcct gtcaatcgcc
     2941 acctctccgc ccctgaggtc gcgtatatcg ttcgagactg cggcgcacgc gcgatcgtcg
     3001 ccagcgcaaa cctcgtcgtc gtcgccgacg ctgccctcca cgacaactcg gtctgcgcgc
     3061 accgtctcgt cgtcggccct gccacctcgg acttcctgtc gtacgacacg gctcttgccg
     3121 gcgagtccga cgagcccttc gaggaccagc cgcgcggcgc gctgatgtgt tactcgtcgg
     3181 gcacgaccgg ccagccgaag ggcatccgcc gtgccttgac cggcaagcag atcgacgacc
     3241 cgaccacggc tgccagcatc gacttgttcg tcgacgtgtt cggcatcgac gcctcgacca
     3301 ggtacctctc acccgcgccg ctctaccatg ccgctcccct cgcctggacg gccgccgttc
     3361 aggctctggg cggcacgacg gtgatcatgg agaccttcga tgcccgccga gcattggtcg
     3421 cgctccacga tcaccgcatc actcacagtc aatgggtacc caccatgttc gtgcggatgc
     3481 tcaagctgcc tgcagcagat cggctcgggc tcgacctgtc gcatcaccgc tgcgcgatcc
     3541 acgccgccgc tccgtgccca cccgaggtga agcggcagat ggtcgattgg tggggcccga
     3601 tcctgtggga gttctacgcg ggcagcgaag gcaacggtgc caccatcatc tcgagtgaag
     3661 agtggctgtc ccgtcccggt tcggtcggac ggccgctgtt cggtgtcgtc cacgtctgcg
     3721 acgacgacgg cacggaggtc gccgtcggcg agacggggct cgtgtatttc gaactgcccg
     3781 gaccggcgtt cgtctaccac ggcgatccag atcgcacgcg cgacacgcgc aacccactgc
     3841 acgaccgatg gacgacgatc ggcgacatcg gccatgtcga ccacgaggga tacctgtact
     3901 tgaccgaccg gcgggcattc acgatcatca gcggtggcgt caacatctac ccgcgcgaga
     3961 tcgaggactg cctcatcatg cacccgtcga tcgccgacgt ggccgtgttc ggcatcccgg
     4021 atgccgacat gggcgaggcg gtgcacgcgt cgatcgagct cgctcgcgat gtcacgccca
     4081 cggccgatct cgacgctgcg ctcatcgcct ttgcccgcca gcgactcgcc ggattcaagg
     4141 tgccgcgcag catcgactac gtcaagtctc tgccgcgcat gccgacgggc aagctcgaca
     4201 agcagccgct ccgtgaacgc tattggaggc aagcacctga ggttgccggg tgagcacggg
     4261 taccggtgcc ctggtcgatc gggtcggggt ccggctcgac gccgacgtca gcggtcacgc
     4321 ccggctgcca ctctctgcgg ccatcgaacg caacggacac gtcctggcgt cgccattgat
     4381 gctcgctgcc gacatcgcct ccgggattcg gctccatcgg ccggacgtcg aatcggtgct
     4441 cacggcgtcc ttctcgtttc gccgcaccga ccgccgccca ctcggcgccg tgaccgccgc
     4501 cgcgacgcga ctgtgggagc acggccggcg agtgatcgac gacgtcgagt acctcgacga
     4561 agcgggcgag caggtcggct ttggacggat cgcgttcacc gtgttgggca gacgtgacgg
     4621 cgggagcgat gccgacggct cggatgctgc agcgttcagc cggttcatga caccgcgcgt
     4681 cgaccccatc gatacgccac tgatcgatgc ggccggcatc gaactggtcg acgctgcggt
     4741 cggccatgcc cggtttcggc cggagcaaga cgtcggggat cggagcgtcg gggatcgagg
     4801 cgtcgcgcca cacgttgttg agcttcgggt cgccgtggan nnnnnnnnng ctccggtgcc
     4861 gacatcgtgc agggaagctt cgtgaccttg ctaggggagg cggccgcact ggccttcgcc
     4921 gaacacgagc tcgccgctgc ggtgacggtg cggtcgctgg aggtgggata tctcgctcct
     4981 gtcgctgccg acaccgtcac cacccgggcg agatggttgg gccgacgcgg agccgccgac
     5041 atcgatgtcg tcgcgtgcga tgtcgagacc ggccgcgtgt gcgcgacatt cgtcgtcggc
     5101 gtgacgaccg tctgaagctg ctggctcgcc cctctcagtt nnnnnnnnnn nnnnnnnnnn
     5161 nnnnnnnnnn nnnnnnnnnn nnncggaaga gcttctcggc attgccgtgc gtcatcgccg
     5221 cgacctcgtc cgacggcagg tctgccagcc ccttgcgcac ctccccttgg ctgtgcggcc
     5281 agggagtgtc ggcgtgtgga tagtcgcact cccacatgat ccggtcgacc ccgatgtgat
     5341 cacgcaactc caggcccgcg tactcgtcga tgaagcagac ccagaagttg cgacggaaca
     5401 gttcggacgg tggcgtgtcg tcgagcttgg cccagtaccg gtgtcgctcc cacgtccggt
     5461 cgatgcgctc gagtgcgaac ggcacccacc cgatcccgcc ttcggagagc gcgatcttga
     5521 tgtcagggaa tcgttgtggg attccggaga ggagcaggtc catgaacgtg atctgagcgt
     5581 tgaccggccc gagcgcgacc gccaccgaaa acggcgcatc cggtgatggc aggtgggtgc
     5641 ggccgctggt gccgatgtga atgcagatca ccgtgtcggt ctcgaccgcg gcctgccaca
     5701 cggggtccca gtgttcggta tggaacgacg gttggtcgaa cggagcggtg ctctccggaa
     5761 agctcacggc acgcacgccg agcgcggcgc agcgacggat ctcggctgcc atcagttgcg
     5821 gatcccacag ctgcccgatc atcatcggga tgtacaggcc tggcaccgct gcgcaccact
     5881 cctcgagcat ccagtcgttg taggccttga cgcacaagtc ggcgagatcc ttgtccttgg
     5941 cggtgaggaa gcgctggccg gcaaaccgag ggaaggtcgg aaagcacaag ctgccccgga
     6001 ccccgtcgtt gagcaggtca cgagcacgct ggatcgggtc gtaacagccg ggaatcatgt
     6061 ccgcgtagct cgtcgggtcc atcgcgaagt ccttgtgctc cttgcctgca acggcgttga
     6121 gccccatcgt ggcgatggcc tccccttcga actcccacgc ctcagtgccg tcggccttgg
     6181 tgatgacgtg ggggccggcg ctctggtact tgctcggcag ccgatccgtc cacacgcccg
     6241 gcggctcgat gatgtgatca tcgacgctga tcagatcgaa atcgtgttgc acgaccatcg
     6301 ttcacccctc tgtcgcgggt tgctcggcga tcggtccgcc atgcgacgcc tcataatata
     6361 gcatactcta ttatggcgtc ccgtacgggt tggccgacgc ggaggagtcg agagatgcga
     6421 gtacgagtgt tggacgatgt ctgccaaggc cacacgctgt gcgcggtcgc tgctccggcc
     6481 gtctttgcct tgcacgaaga ggacggccgc agttacgtca tcgtcgacga ggtgccggcc
     6541 gagcacgaag cggcggcgcg gcgagctgag atcggctggc cc
//
LOCUS       DAORNF010000082         6555 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_8719_length_6555_cov_2.010769, whole genome shotgun sequence.
ACCESSION   DAORNF010000082 DAORNF010000000
VERSION     DAORNF010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 6555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6555
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8719_length_6555_cov_2.010769"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="PLV68_04660"
     CDS             <1..280
                     /locus_tag="PLV68_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00567.1"
                     /translation="LESPESGETNQTLFTCDLLSILFAGLAAAGDVLTRESLVAGLET
                     ITDFPLALWGNLTFSSNDHAGVEQVRTTKWSTACRCWSALDDFADPLP"
     gene            318..1787
                     /locus_tag="PLV68_04665"
     CDS             318..1787
                     /locus_tag="PLV68_04665"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRE00568.1"
                     /translation="MTNIPDVRIPSGSLLIGDGRIDRTSAGDIEQLDPSRGTPLAAIT
                     LAGPDEIDAAVRAAKTAEPAWRSMAPNRRRDLIMAFADALDAHHEELSILRSLELGAP
                     KKQGKGLNMAVEYMRYFAGWVDKLEGTTIPLGSGVLNYTVPEPYGTIAALTPWNGGVV
                     SAAMKVVPALVAGNCVVLKPSELAALAPLRFGELALEAGLAPGVLNVVPGGVAAGEAL
                     VAHPGVGKISFTGGTVTARRVLAGAATNLTPVILELGGKSANVVFADADLDAAVATTI
                     SVALAGMSGQGCVLPTRVLIQDSVYDEVEQRVVAAAAALVVGRPFEPGVQAGPVISAQ
                     SLDRILGMIERARDRGDGELKTGGHRLLGDLSSGFFVAPTVFGSVDNASPIAQEEVFG
                     PVISLVRFTDEADAIRLANESVYGLGGLVFTNDIGRAHRVAHQLDVGSVGVNAFAPMP
                     PSAPFGGVKQSGFGREGGLVGVREFLRDKNVYVGLGAPR"
     assembly_gap    1835..1878
                     /estimated_length=44
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1879..2073)
                     /locus_tag="PLV68_04670"
     CDS             complement(<1879..2073)
                     /locus_tag="PLV68_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00569.1"
                     /translation="MVEGLGPQQAIDMPRFKICFGGELALEPNHPLASRYPAALDKNP
                     GPEGFGAAQMIGWHRDELLAG"
     assembly_gap    2088..2097
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2098..3250)
                     /locus_tag="PLV68_04675"
     CDS             complement(<2098..3250)
                     /locus_tag="PLV68_04675"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HRE00570.1"
                     /translation="MSAAIATSDPRASAAGADVLRAGGNAVDAAATAAFVLYVVEPQS
                     CGIGGDAFMLIHDGSGPPLGLDGSGLVPVGLTRAALEADGLDNVPARGAKTATPPGAV
                     ALLAHALAGHGTISLAEAIAPALRFAADGFAVRPSLADAARRATAELADQPVLGPLYW
                     PGGVPVGVDDIVVNHDLASALRVVAAQGGQALTTGSLAVAIVEAIEAQGGYLTSADLA
                     AHRTTSITPESVEFAGHRVWQMPAPTQGPAVLEALAALSSTEPVDWEAAYRAVASGMA
                     AAGFDPAAVRVGTPSPAKGDTTFIAAVDRDGVGVSLITSVFGDFGSHLGIRALGGPIH
                     NRATTFRLSTRPIQPGKPPHTTIPGMITNADGSLAYVMGVAGGVMQPQAQ"
     gene            complement(3247..>3978)
                     /locus_tag="PLV68_04680"
     CDS             complement(3247..>3978)
                     /locus_tag="PLV68_04680"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRE00571.1"
                     /translation="YIADYCAADDDRLKAALLLPAAAPAEAVRMIKEYGDAKWVAAAS
                     VVLPEGTPIDEPDLNPIWSAMGDYDLPLLHHSFFYEPPYFPGYRDIWGNVVIARAAAH
                     PWGAQRLLAYLTLSGMFDAFPNLRIGFAECSAGWLPSWLNRLEGQASYMASKVPYRKM
                     TPTEYAQNGRIFCGIELYEGDAIAKSTIDLLGDGVLMYSSDYPHNQAEFPSSPDIVLT
                     WEPTLGRDNMTKLMSTNAERYMRIL"
     assembly_gap    3979..3991
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3992..4410)
                     /locus_tag="PLV68_04685"
     CDS             complement(<3992..4410)
                     /locus_tag="PLV68_04685"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00572.1"
                     /translation="MKSGYKIIDTDTHVGPNLETLHLYASDALKDRWSEFDPYLQPVT
                     LGGHFLSVSPMRYPRDLGTEQQGATIVKGGDSPLRGAVTKGFVQPPADEVSNLNAVGR
                     LADMDLEGVDVNLVIPGTFGNAATTFDTALAVEIYDA"
     assembly_gap    4572..4581
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4582..5208)
                     /locus_tag="PLV68_04690"
     CDS             complement(<4582..5208)
                     /locus_tag="PLV68_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00573.1"
                     /translation="MVKWTRAVAVVAAGALALASCGSDDGDSSPTSTAPVTTQAEVTT
                     TTAAPTTTADNSAASDEVAANVVELFDQLAIAGNPDTSPADAEAAVAAAAALIEGGDS
                     EEVLAQVPRIAGLAAAAQLTIVIDEAPVIDGDTATYKFSALSFGNPSQLNKANGVSVL
                     ENGVWKLSSDIWAAFLAMGGDSNPEDMGGGDDDSAASEEVAANVVELFD"
     gene            5428..>6106
                     /locus_tag="PLV68_04695"
     CDS             5428..>6106
                     /locus_tag="PLV68_04695"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRE00574.1"
                     /translation="MTWPWLDKGFTSDRHRWSGDAHRDRRALHDVERYTTTEFRAETA
                     GLGVIGLVHAHAATAADPAHETAWLGELGDRLGWPSAIIARAELASPGCVDLLRRHRA
                     ASPRCSSVRDMGGPDGLDLDACAAALDVATELQFAVEVRAAPERFAMFDELAQRWPEV
                     NFVLSHASLPIERTPSTLRLWRDAALRLAEHPNWYCKVSALCGGSDPDWTVESIRPGA
                     LGCVETFG"
     assembly_gap    6107..6336
                     /estimated_length=230
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6337..6519)
                     /locus_tag="PLV68_04700"
     CDS             complement(<6337..6519)
                     /locus_tag="PLV68_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00575.1"
                     /translation="MYSLFVDHTDRVYLGAGGRLLRSDDLATTWTVLEDGLDGVSVYE
                     VCERPDGLLVIATSGGL"
BASE COUNT          992 a         2013 c         2139 g         1104 t
ORIGIN      
        1 gctcgaatca ccggagagcg gcgagacgaa tcagacgctg ttcacgtgcg atctgctgtc
       61 gatcctgttc gcggggttgg ccgcggccgg tgatgtgctg acccgcgaga gcctcgtcgc
      121 agggctggag accatcacgg actttcccct cgcgctgtgg ggcaatctga cgttcagctc
      181 gaacgaccat gccggtgtcg aacaagtgcg caccacgaag tggtccacgg catgccggtg
      241 ctggtcagcg ctcgacgact tcgccgaccc cttgccatga catccgacct cgccgaccaa
      301 caaggacgac tccgcccatg accaacatcc ccgacgttcg catcccctca ggctcgctgc
      361 tgatcggcga tggacgcatc gatcgcacct cggccggaga catcgagcaa ctcgatccgt
      421 cgagaggtac gccgcttgcg gcgatcacgc ttgcagggcc ggatgagatc gacgcggccg
      481 tccgcgctgc caagactgcc gaaccggcct ggcggtcgat ggcgccgaac cggcggcgcg
      541 acctgatcat ggccttcgcc gacgccctcg acgcccacca cgaggagctg tcgatcctgc
      601 gctcactcga actcggagcc cccaagaagc agggtaaggg cttgaacatg gcggtcgagt
      661 acatgcgcta cttcgccggg tgggtcgaca agctcgaagg cacgacgatc ccgctcgggt
      721 cgggcgtgct gaactacacg gtgcccgagc cgtacggcac gatcgccgcg ctgaccccat
      781 ggaacggcgg agtggtgtcg gccgcgatga aggttgtgcc cgcgctcgtc gccggaaact
      841 gcgtcgtatt gaagccgtcg gagttggcgg cgctcgcgcc cttgcgattc ggggagttgg
      901 cgctcgaagc cgggttggcg cccggggtgt tgaacgtggt tccgggtggg gtcgccgcag
      961 gtgaggcgct ggtcgcccac cccggtgtcg gcaagatcag cttcaccggc ggaaccgtca
     1021 cggctcggcg ggtcctcgcg ggtgcggcta ccaacctgac gccagtgatc ctggagctcg
     1081 gtggcaagtc ggccaacgtc gtctttgccg acgccgatct ggatgctgcg gtggcgacga
     1141 cgatctcggt cgcgcttgcc ggcatgagcg gccagggttg cgttctgccg acgcgcgtgc
     1201 tgattcagga cagcgtctac gacgaggtcg agcaacgcgt cgtcgccgct gctgcggcat
     1261 tggtcgtcgg ccgtccgttc gagccaggtg tgcaggccgg cccggtcatc tcggcgcagt
     1321 cgttggaccg gatccttggc atgatcgagc gagcgcgtga tcgcggcgac ggcgagctca
     1381 agacaggtgg ccatcggctg ctgggtgatc tgtcatccgg gtttttcgtc gcgccgaccg
     1441 tgttcggcag tgtcgacaac gcgtcgccga tcgcgcaaga ggaggtcttc ggtccggtca
     1501 tcagcctggt tcgcttcacc gatgaagccg acgcgattcg tctcgccaac gaatccgtct
     1561 acgggctcgg tggtctcgtg ttcaccaacg acatcggccg cgcccaccgc gtcgcgcacc
     1621 aactcgacgt cggttctgtg ggggtcaacg cgtttgcccc aatgccgccg agcgcgccgt
     1681 tcggtggggt caagcagagt ggcttcgggc gtgaaggtgg gttggtgggt gtgcgcgagt
     1741 tcctgcgcga caagaacgtc tacgtcggcc tcggagcacc gcgctgacgc cgctggtcgc
     1801 gggctgacgc ggaccggtca ggtgtcgatg acttnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnac ccgccaagag ctcgtcgcga tgccacccga tcatctgcgc
     1921 ggcgccgaag ccttctggcc cagggttctt gtcgagcgcg gccggatagc gagatgcaag
     1981 tggatgattc ggctcgagag ccaactcgcc accgaagcag atcttgaacc ggggcatgtc
     2041 gatggcctgc tgcggcccga ggccctcgac caacgcgcga atcgccannn nnnnnnncct
     2101 gggcctgggg ctgcatcaca ccgcctgcca cgcccatgac gtaggcgagc gatccgtccg
     2161 cgttggtgat catgccggga atcgtcgtgt gcggcggctt cccgggttgg atcggacgag
     2221 tcgagagccg gaaggtggtg gcgcggttgt ggatcggccc gccgagtgcc cggatcccga
     2281 gatgcgaccc gaagtcgccg aacaccgacg tgatcaacga gacgccgact ccgtcgcgat
     2341 cgaccgcggc gatgaaggtc gtgtcgccct tggccgggct gggtgtgccg acgcggaccg
     2401 cggccgggtc gaatccggcc gccgccatcc ccgatgccac cgcccggtac gcagcctccc
     2461 aatcgaccgg ttcggtgctg ctgagggcgg cgagcgcctc gagcacggcg ggtccctggg
     2521 tgggggctgg catctgccac acccgatggc ccgcgaactc gaccgactcg ggggtgatcg
     2581 aggtggtgcg gtgtgctgcc aggtcggcag aggtcaggta tccgccttgt gcttcgatcg
     2641 cctcgacgat ggccacggcg agcgaacctg tggtcagcgc ttgtccaccc tgggcagcca
     2701 cgacgcggag agcggaagcg agatcgtgat tgaccacgat gtcgtccacg ccgaccggga
     2761 caccgccggg ccagtagagc ggaccgagca ccggctggtc cgccaactct gcggtggccc
     2821 gacgcgcggc gtcggccagg ctgggccgaa cggcgaagcc gtcagcagcg aatcgcaggg
     2881 cgggagcgat cgcttccgca aggctgatcg tgccatgccc tgcgagtgcg tgcgcgagga
     2941 gcgcaactgc accggggggc gtcgcagtct tggctcctcg ggcgggaacg ttgtcgagac
     3001 cgtcggcttc gagcgcggca cgggtgaggc cgaccggcac caatcctgat ccatcgaggc
     3061 cgagcggcgg gccggacccg tcgtggatga gcatgaaggc gtcgccaccg atgccgcacg
     3121 actggggttc gacgacgtac agcacgaatg cggccgtcgc cgcggcgtcg accgcattgc
     3181 cgcctgcgcg cagaacatcc gcgcccgctg cggacgccct cggatccgac gtggcgatgg
     3241 cggcgctcac agaattcgca tgtaccgttc ggcgttcgtc gacatgagct tggtcatgtt
     3301 gtcgcgtccg agcgtcggtt cccatgtcaa cacgatgtca ggagatgacg gaaactcggc
     3361 ttgattgtgc gggtagtccg aggagtacat cagcacaccg tcgccgagca ggtcgatagt
     3421 cgacttggcg atcgcgtcgc cctcgtacag ttcgatcccg cagaagatgc ggccgttttg
     3481 cgcatactcg gtgggcgtca tcttgcggta ggggaccttg ctggccatgt agctggcttg
     3541 gccttcgagg cggttgagcc acgagggcag ccagccggcg ctgcactcgg cgaagccgat
     3601 ccgcagattc ggaaacgcgt cgaacatgcc cgacagggtg agataggcga gcaatcgctg
     3661 agctccccac gggtgggctg cggctcgggc gatgacgacg ttgccccaga tgtcgcggta
     3721 gccggggaag tacggcggtt cgtagaagaa ggagtggtgc agtaacggca ggtcgtagtc
     3781 gcccatggcc gaccagatcg ggttgaggtc gggctcgtcg atcggtgtcc cctccggcag
     3841 cacgacggac gccgcggcga cccactttgc gtcgccgtac tccttgatca tccgaacggc
     3901 ttcggcgggt gctgcggcgg gcagcagcag cgcggctttg agtcgatcgt cgtcagccgc
     3961 gcagtagtcg gcgatgtann nnnnnnnnnn ntaggcgtcg tagatctcga cagcgagcgc
     4021 cgtgtcgaac gtcgtcgcgg cgttgccgaa cgtgccgggg atcacgaggt tgacgtcgac
     4081 gccttcgaga tccatgtcgg caaggcgtcc gacggcgttg aggttcgaca cctcgtcggc
     4141 cggcggttgc acgaacccct tggtgaccgc gccgcgcagc ggcgagtcac cgcctttgac
     4201 gatggttgca ccctgctgct cggtgccgag atcgcgcggg taccgcatcg ggctgacgct
     4261 gaggaagtgc ccgccgagag tcaccggctg gaggtacggg tcgaactctg accatcgatc
     4321 cttgagcgcg tccgacgcgt acaagtgcag tgtctccaag ttcggcccga cgtgggtatc
     4381 cgtatcgatg atcttgtacc cactcttcac aggggtcctc ccattcaggt gctgtctact
     4441 aaatcagtgt actcaatggt actgccgcca gtactctcgt gccaggggtt gtcgggaaaa
     4501 gtctgcgtcg ggtgcagtcg cgacgatgcc caggccgccg tcgcggcctg ggcatcgtga
     4561 tgttctccgg gnnnnnnnnn natcgaacaa ctccacgacg ttggcggcaa cctcctcaga
     4621 cgccgccgaa tcgtcgtcgc ccccgcccat gtcttctggg tttgagtcgc cgcccatggc
     4681 gaggaacgcg gcccagatgt cggatgacag cttccagacg ccgttctcga ggacgctgac
     4741 gccgttggcc ttgttgagtt gtgacgggtt gccgaacgac agcgcgctga acttgtaggt
     4801 ggcggtgtcg ccgtcgatca ctggtgcttc gtcgatgacg atggtcagct gtgccgcggc
     4861 ggcaagacct gcgatgcggg gcacctgggc cagcacttct tcactgtcac cgccttcgat
     4921 cagcgctgct gcggcagcaa ccgctgcctc cgcgtctgcc ggtgatgtgt ccgggttgcc
     4981 cgcgatcgcg agttgatcga acaactccac gacgttggcg gcaacctcgt cagacgccgc
     5041 cgagttgtcg gccgtcgtcg tgggcgcagc ggtcgtcgtc gtcacctctg cctgtgtcgt
     5101 gaccggcgcg gtcgacgttg gcgacgagtc gccgtcatca ctcccgcacg atgcgagcgc
     5161 gagtgcgccc gcagcaacga cggctaccgc cctggtccat ttcaccatga cttcccccta
     5221 atcctgtgtg gatgttcggg tcggcggcgc cgacccttgg tcgacgctca tcagccgtcc
     5281 cacaaaagag tatacttcat tattggacgc cagggaaggg cagacttctg ccagcagccg
     5341 accactcggg cccgtcgatg ccagatgcca cacaccacgc agcttctcga tacgcatgtc
     5401 cacttctggg acagatctcg atccgacttg acgtggccgt ggctcgacaa gggattcacc
     5461 agcgatcgcc atcggtggtc cggcgacgct caccgagacc gacgcgcact gcacgacgtc
     5521 gagcgctaca ccaccaccga gttccgcgcg gagacagcag gactcggcgt gatcggactg
     5581 gtccatgcac atgcagcgac agcggccgac cctgcccacg agacggcgtg gcttggtgag
     5641 ctcggcgacc gactcgggtg gcccagcgcg atcatcgccc gcgccgagct tgcctccccc
     5701 ggttgtgtcg atctgctccg ccgccaccgt gcggccagcc cccggtgctc cagcgttcgc
     5761 gacatgggcg gcccggatgg cctcgatttg gatgcgtgcg cggccgcgct cgacgtcgcg
     5821 accgagttgc agttcgcggt cgaggtccgc gcggctcctg aacgctttgc gatgttcgac
     5881 gaactggcac aacggtggcc cgaggtcaac ttcgtgttga gtcacgcgtc gttgcccatc
     5941 gagcgcacgc cgtcgacact tcggctgtgg cgcgacgcgg cgctgagact ggctgagcac
     6001 cccaactggt actgcaaggt gtccgccctg tgcggcggct cggatcccga ctggacggtc
     6061 gagtcgattc ggccgggggc cctcggctgt gtggagacgt tcggggnnnn nnnnnnnnnn
     6121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncagg ccaccgctgg ttgcgatgac
     6361 aagcaaccca tccggtcgtt cgcagacctc gtagacgctg acgccgtcga gaccgtcctc
     6421 gagcacggtc caggtcgtgg cgaggtcgtc gctgcgcagc agtcgacctc ctgcacctag
     6481 gtagacgcgg tcggtgtgat cgacgaacag cgagtacatc ttcgcctctt ggccgagtgg
     6541 tagtgggcat ggatg
//
LOCUS       DAORNF010000083         6536 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_8760_length_6536_cov_1.879802, whole genome shotgun sequence.
ACCESSION   DAORNF010000083 DAORNF010000000
VERSION     DAORNF010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 6536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6536
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8760_length_6536_cov_1.879802"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="PLV68_04705"
     CDS             complement(<1..268)
                     /locus_tag="PLV68_04705"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00576.1"
                     /translation="MIGNYVMRQKDLQDERTKADTIGAAREYLLGQNLFPTKLAEHRG
                     ALSFELTKEKVKKKELMHFTRQLAVFVKAGIPLMDAIQTIGDETE"
     assembly_gap    324..379
                     /estimated_length=56
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(398..>789)
                     /locus_tag="PLV68_04710"
     CDS             complement(398..>789)
                     /locus_tag="PLV68_04710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00577.1"
                     /translation="FLIASSVVGVIGQRLLRRICDSCKEPYTPGPDELAVFRQHSGGS
                     EKTQFFHGAGCNYCAGTGYRDRIGVYELLRITPELRRLIVGWATTEELRRLAVAQGMR
                     TMLREAMALVESDVTTVPEVVRTLFAH"
     assembly_gap    790..963
                     /estimated_length=174
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<964..2186)
                     /locus_tag="PLV68_04715"
     CDS             complement(<964..2186)
                     /locus_tag="PLV68_04715"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HRE00578.1"
                     /translation="MALFTKTKDGARDGATLDAPDSTSNAARVPEPGPSAAECMQVGQ
                     LLVDAEQLKPEALASTLQIANGDLLQFADLAIARFGCGRTEMAMAIGQVLGVPAADTK
                     GIELDPAVIALVDEKTCRAHRVIPVSKDGNTVTMLAADPSPYRRAAVEAALDGMQVTW
                     FVGDAPTVKTFIEKSFRADADVERLAAAFGNTEDQTKAVAEAAAVAAADDQAPIVQLV
                     NRIVGQAMRDRTSDIHVEPMDETLRIRFRIDGNLVEAFSLPIGVHAALISRLKVMSGM
                     NIVERRRPQDGQFSTVIDGKEVDVRVASVATVWGEKIVMRILDKGRSMIGLGELGFPR
                     DTYLQYSKMVHSPFGMVICAGPTGAGKTTTLYATLQEINSAGKNLVTVEDPVEYVFPG
                     INQIQTNEQAGLTFAT"
     gene            complement(2216..>2585)
                     /locus_tag="PLV68_04720"
     CDS             complement(2216..>2585)
                     /locus_tag="PLV68_04720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00579.1"
                     /translation="AEQIASIDHDAIDQLREQMSSAIGESMLVRIELERVASSSTEEF
                     DKIHVRMAELANDIAESTLDVGTAVQLERLEEIERALIELNPDQFVRKNDPDHAGDTG
                     RADDTGRADDGAGGASASAS"
     assembly_gap    2586..2668
                     /estimated_length=83
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2669..>3076)
                     /locus_tag="PLV68_04725"
     CDS             complement(<2669..>3076)
                     /locus_tag="PLV68_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00580.1"
                     /translation="ISEAVVDERVGSALQQVQQRMDRTAEALAARIDELHAATARFDE
                     QAAAIVAHVNDTTATLGRHLDETGKVLAAHVEEQTTAAHQQVQEQLQAFGRKIDDTEN
                     RLVDRSLAMEERINEQTGVRLAAVEATIGRISGG"
     assembly_gap    3077..3086
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3087..3391)
                     /locus_tag="PLV68_04730"
     CDS             complement(<3087..3391)
                     /locus_tag="PLV68_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00581.1"
                     /translation="MAGNQATASGTGTLVVDDAPSPSPATQPAPAPPPGLADAQLAAI
                     LTAVEQQLTRYFSAMAARTDAVQHAGEASRNELATHIQRQLDVLASELAIAQDAAAGA
                     "
     gene            3544..>4172
                     /locus_tag="PLV68_04735"
     CDS             3544..>4172
                     /locus_tag="PLV68_04735"
                     /inference="COORDINATES: protein motif:HMM:NF014759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease"
                     /protein_id="HRE00582.1"
                     /translation="MPGPNSSATQPPTTGIRVHLVDGTYELFRQHYGQMSHRGDAGPY
                     AATVGVLASTLQLLSGGANHAAATHVGVASDHVIESFRNDLWPGYKTSAGMDPDLLAQ
                     IPVVEDALEAMGITTWRMVSHEADDALGAAAALADADPQVEQALIVTPDKDLGQCVRG
                     QRVVQYDRRKDEIIDEAAVRAKFGVGPDLIADYLGLVGDTADGFPGLPG"
     assembly_gap    4173..4223
                     /estimated_length=51
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4224..4457
                     /locus_tag="PLV68_04740"
     CDS             <4224..4457
                     /locus_tag="PLV68_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012226157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease"
                     /protein_id="HRE00583.1"
                     /translation="EWAPVGVRGAAKLAGTLAEQFDLALLFRRIATVVTDLDVGAVHE
                     WRWTGPTGEFAAIASRLGAAYLVARAESIAARR"
     gene            complement(4511..4609)
                     /gene="ffs"
                     /locus_tag="PLV68_04745"
     ncRNA           complement(4511..4609)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PLV68_04745"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            4679..4766
                     /locus_tag="PLV68_04750"
     tRNA            4679..4766
                     /locus_tag="PLV68_04750"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4713..4715,aa:Ser,seq:gga)
     gene            4985..5503
                     /gene="tadA"
                     /locus_tag="PLV68_04755"
     CDS             4985..5503
                     /gene="tadA"
                     /locus_tag="PLV68_04755"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: protein motif:HMM:NF008113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HRE00584.1"
                     /translation="MDVADDAVAMGVAVAQARLAELHADVPVGAVVVRDGAVIAARHN
                     ERELTGDPTAHAEVLAIRDAAEAVGSWRLDGCTLYVTLEPCPMCAGALVNARVPRVVY
                     GATDPKAGAGGSLMNLLADPRLNHRCEVTAGIMADESSALLKAFFAGRRRNAGSSSTA
                     TDDEAGCTGPSR"
     gene            complement(5619..>6536)
                     /locus_tag="PLV68_04760"
     CDS             complement(5619..>6536)
                     /locus_tag="PLV68_04760"
                     /inference="COORDINATES: protein motif:HMM:NF036834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00585.1"
                     /translation="RYITDRFLPDKAIDLVDEAASRLRMEIDSKPAELDVVDRDIMQR
                     EIEREALKKEKDKASKERLKKLEEELANLKEHSTQLTARWQAEKEAIERIQSVKEEID
                     QVNVQIEQAQRAMDYQKAAELQYGRLHALEKSLKQASDKLTAMQQDGAMLKEEVGAEE
                     IAQIVAKWTGIPVSKLLEGEVEKLVLMEDRLHDRVVGQDEAIRAVAAAVRRAIKAIEA
                     ATAASPQTPAVTFGAMHVRDAMPATFESVVAPTLAAATAAPSGAPTDVFASTAPTGEL
                     VAMATATDVIGSLYLAGEVLGADRATWPD"
BASE COUNT          872 a         2010 c         2117 g         1163 t
ORIGIN      
        1 cttcggtctc gtcaccgatg gtctggatcg cgtccatcag cggaatgccg gccttgacga
       61 acacggcgag ctgacgcgtg aagtgcatca gctccttctt cttgaccttc tccttggtga
      121 gctcgaagct gagtgcgccg cggtgttcgg cgagcttggt cgggaacagg ttctgaccca
      181 ggaggtattc gcgcgccgcg ccgatggtgt cggccttggt gcgttcgtct tgcagatcct
      241 tctggcgcat gacgtaattg ccaatcattc gtcgcgcctc acgcacataa agctggtggg
      301 gccagtgtcc cgtatcgaca aacnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnc gtcggcccaa tggcggccta gtgggcgaac agggtgcgga
      421 ccacttcggg cacggtggtg acgtcggatt cgaccagcgc catggcttcg cgcagcatcg
      481 tccgcatccc ttgggccacc gccagacggc ggagctcttc ggtcgtggcc cagccgacga
      541 tcaggcggcg gagctcgggg gtgatgcgca gcaactcgta gacgccgatg cggtcgcggt
      601 agccggtgcc ggcgcagtag ttgcacccgg cgccgtggaa gaactgggtc ttctcgctgc
      661 caccgctgtg ctggcggaac accgcgagct cgtcgggtcc tggtgtgtac ggctccttac
      721 agctgtcgca gatgcggcgc aacagacgct ggccgatcac gcccaccacc gacgaggcga
      781 tgaggaaggn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      961 nnngtggcga aggtgaggcc cgcctgctcg ttggtctgga tctggttgat gcctgggaag
     1021 acgtactcga cggggtcctc gacggtgacc aggttcttgc ctgccgagtt gatctcctgc
     1081 agggtggcgt acagcgtggt ggtcttgccg gcaccggtcg gtccggcgca gatcaccatc
     1141 ccgaagggtg agtggaccat tttcgagtac tgcaggtagg tgtcgcgggg gaaccccaac
     1201 tcgccgaggc cgatcatcga gcggcccttg tcgaggattc gcatgacgat cttctcgccc
     1261 cacaccgtgg ccacgctggc gacgcggacg tcgacctcct tgccgtcgat cacggtggag
     1321 aactggccgt cctgggggcg gcggcgttcg acgatgttca tgccgctcat caccttgagg
     1381 cggctgatca gcgcggcgtg gacgccgatc ggcaggctga acgcttcgac caggttgccg
     1441 tcgatgcgga agcggatgcg cagggtctcg tccatcggct cgacgtggat gtccgaggtg
     1501 cggtcgcgca tcgcctggcc gacgatgcgg ttgaccaact gcacgatcgg cgcttgatcg
     1561 tccgctgcgg ccaccgcggc agcctcggca acggccttgg tctggtcctc cgtgttgccg
     1621 aaggcagcgg cgagacgctc gacgtcggca tcggcgcgga acgacttctc gatgaaggtc
     1681 ttgacggtcg gcgcgtcacc gacgaaccaa gtcacctgca tgccgtccag tgcggcctcg
     1741 accgcggcgc ggcggtacgg cgacgggtcg gccgccagca tcgtgacggt gttgccgtcc
     1801 ttggacaccg ggatgacccg atgggcgcgg catgtcttct cgtcgaccag cgcgatgacg
     1861 gcggggtcga gctcgatgcc cttggtgtcg gctgccggca cgccgagcac ctgaccaatc
     1921 gccatcgcca tctcggtccg accgcagccg aagcgggcga tcgccaggtc ggcgaactgc
     1981 aacaggtcgc cgttggcgat ctgcaacgtc gaagccagcg cctcgggctt cagctgctcg
     2041 gcgtcgacca gcagttggcc gacctgcatg cactcggccg cgcttggtcc cggctctggg
     2101 actcgggccg cgttggatgt gctgtccggc gcatcgagcg tggcgccgtc ccgggctccg
     2161 tctttggttt tggtgaacag cgccatcggt gcctctccgc ctggtcagat ggtggtcaac
     2221 tcgccgaggc cgacgcgccg cccgcaccgt catctgctcg gcccgtgtcg tctgctcggc
     2281 ccgtgtcgcc tgcatgatca gggtcgttct tgcgcacgaa ctggtcgggg ttcaactcga
     2341 tcagggcgcg ctcgatctct tccaagcgtt cgagctgcac cgcggtgccg acgtcgaggg
     2401 tggattcggc gatgtcgttg gccagctcgg ccatgcggac gtggatcttg tcgaactctt
     2461 cggtcgagct cgatgcaacc cgctccaact cgatacggac gagcatcgac tcgccgatcg
     2521 cgctcgacat ctgctcccgc agctggtcga ttgcgtcgtg gtcgatgctg gcgatttgct
     2581 cggccnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnngc caccgctgat gcgtccgatg gtggcttcga
     2701 cagcggcgag gcgaaccccg gtctgctcgt tgatgcgctc ttccatcgcc agcgaacgat
     2761 cgacgaggcg gttctcggtg tcgtcgatct tgcggccgaa tgcctgcagc tgctcttgca
     2821 cctgttgatg ggcagcggtg gtctgctctt ccacgtgggc cgcgagcacc ttgccggtct
     2881 cgtcgaggtg ccggccgagc gtggccgtcg tgtcgttgac gtgggcgacg atcgctgccg
     2941 cctgctcgtc gaagcgcgcc gtcgccgcgt gcagctcgtc gatccgagcc gccagcgcct
     3001 cggccgtgcg gtccatccgc tgctgcacct gctgcagggc gctgccgacc cgttcgtcga
     3061 ccaccgcctc gctgatnnnn nnnnnngccc cggctgctgc gtcctgggcg atggccagct
     3121 ccgatgccag cacgtcgagt tggcgttgga tgtgggtggc gagctcgttg cgactggcct
     3181 cgccggcatg ttggaccgcg tcagtgcggg ccgccatcgc cgagaagtag cgggtgagct
     3241 gctgttcgac cgctgtcaag atcgcggcaa gctgcgcgtc ggccaacccg ggcgggggcg
     3301 cgggcgccgg ctgggtcgcc ggtgaaggtg atggcgcgtc gtccacgacc aacgtgcccg
     3361 ttccacttgc cgtcgcctgg ttccctgcca cggcttaacc atcgtcagtg ttgccgcaat
     3421 cttgagaacg acaagcgtgt aacttcgccg tttcttgacg aaacttctcg cacggcgcgt
     3481 gcaatgtgtt cgccgccgcg catcgagcca ggtcgtggcg tgagccgtgc cagactcgga
     3541 cctgtgccgg gaccgaactc atcagccaca cagccgccga cgacgggcat tcgggttcat
     3601 ctcgtcgatg ggacctacga actgttccgg cagcactacg ggcagatgag ccaccgcggg
     3661 gacgctggtc cgtacgcggc gacggtgggg gtgctggcgt cgacgttgca gctcttgtcg
     3721 ggtggcgcga accatgccgc ggcgacccac gtcggcgttg ccagcgacca cgtgatcgag
     3781 agcttccgta acgacctctg gcccggatac aagaccagcg cagggatgga cccagacctg
     3841 ctcgcccaga tccccgtcgt cgaggatgcg ctggaggcga tggggatcac cacatggcgc
     3901 atggtcagtc acgaagccga cgacgcactc ggtgcggccg cggcgctggc cgatgccgac
     3961 ccgcaggtgg agcaggcgtt gatcgtcacc cccgacaagg acctgggcca atgcgtgcgc
     4021 ggccagcggg tggtgcagta cgaccggcgc aaggacgaga tcatcgacga ggcggcggtg
     4081 cgggccaagt tcggcgtcgg accggacctg atcgccgact acctcggtct ggtcggtgac
     4141 acggccgacg ggtttcccgg cttacccggc ttnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4201 nnnnnnnnnn nnnnnnnnnn nnngaatggg cgccggtcgg cgtgcgcggg gccgcgaagc
     4261 tggctggcac cctggccgag caattcgacc tggcgctgct gttccgccgc atcgccaccg
     4321 tggtcaccga tctcgacgtc ggcgcggtgc acgagtggcg ctggaccggc ccgaccggcg
     4381 agttcgccgc gatcgcctcc cggctgggtg ccgcctacct ggtcgcgcgg gccgagtcga
     4441 tcgcggctcg ccgctgagac gatcgacggt gggggcgccc cggtggggcg tcgctcccgc
     4501 tgagtgtggc gacggccagg cgatctgcgg cacacagtcg ttgccgttga aggctgctcc
     4561 atccctggcc tgacctggtt cccggactcc cgttgcacag gacccgaccg tcaccacacc
     4621 cagcgggtaa ccaacggtgg cgtcagacta ccgggccgct accctgctgg cactcccagg
     4681 agggttgcga gagcggccga atcggcacgc ctggaaagcg tgtgacctgc aaaggtccgt
     4741 gggttcaaat cccacaccct ccgccaattc cctccgccac ttcgtcgtgt cgcacggcgc
     4801 ctgtccgggc gcgtccagga cgcggaactt cgcggtgccg atggttgccg tgaacgtctg
     4861 aacttcgggc tgaaccgttg ggcagagcgt ctcgccggga tgtcgagcgg tgttcgcctt
     4921 gctgtgctcg gcgggtgcgt gaggcgattg gtagggcgag actgggggca tgcaggctgg
     4981 gggagtggac gtcgctgacg atgcggtggc aatgggcgtg gcggtcgcgc aggcgcggtt
     5041 ggccgaactg cacgccgatg tgccggtcgg agcagtggtg gtgcgtgacg gggcggtgat
     5101 cgcggcgcgt cacaacgaac gcgagctcac cggcgatccg acggcgcacg ccgaggtgct
     5161 cgccatccgc gatgcggccg aagcggtggg aagctggcgc ctcgacgggt gcacgctgta
     5221 cgtcaccctc gaaccgtgcc cgatgtgtgc cggtgccctg gtcaacgctc gggtaccgcg
     5281 cgtggtctac ggggccaccg accccaaggc tggggcgggc ggcagcctga tgaacctgct
     5341 cgccgacccg cggctcaacc accgttgcga ggtcaccgcc ggcatcatgg ccgacgagag
     5401 ctcggccttg ctcaaggcgt tcttcgccgg gcgtcgccgc aacgctggct ccagcagtac
     5461 ggcgacggac gacgaagcgg gctgcaccgg cccctcacgc tgaccgacgg tgcgggtcag
     5521 ctgctgcggc ggcggatgcc actgatcagg cgacgcagag caccgcggcg ctcgcggctg
     5581 atcggcgcgg cgatgggcat gtggctgccc cgtctgatct agtccggcca ggtggcacgg
     5641 tcggcgccca gaacctcgcc cgccagatac agcgagccga tgacatcggt ggcggtcgcc
     5701 atggcgacga gttcgccggt cggggcggtg ctcgcgaaca cgtcggtcgg tgcgccgctc
     5761 ggggcggctg ttgcagcggc gagcgtgggg gcgaccaccg actcgaacgt cgcaggcatc
     5821 gcgtcccgga cgtgcatcgc tccgaacgtc accgctggcg tctgaggcga cgcggcggtc
     5881 gctgcttcga tcgccttgat cgcccggcgc accgcggcgg ccacggcccg aattgcttcg
     5941 tcctggccca ccacgcggtc gtgcaggcga tcttccatca aaaccagctt ctccacttcg
     6001 ccttcgagca gcttgctcac cgggatgccg gtccatttgg ccacaatttg ggcaatctct
     6061 tctgcgccca cttcttcttt gagcatcgcg ccgtcttgct gcatggcggt tagcttgtcg
     6121 ctggcttgtt tgagtgattt ttctagggcg tggagacggc cgtattgcaa ttccgccgcc
     6181 ttctggtaat ccattgcccg ctgtgcctgc tcaatctgca cgttcacctg gtcaatctct
     6241 tccttcaccg actggatgcg ctcgatggct tctttctcag cttgccaacg ggctgtcagc
     6301 tgcgtgctgt gctctttcag gttcgccagc tcttcttcca gcttcttgag ccgttctttg
     6361 ctggccttat ctttctcttt tttcagcgcc tcacgctcga tttcccgctg catgatgtcg
     6421 cgatcgacga catccagctc agctggtttg ctgtcgattt ccatgcgcag gcggctggcg
     6481 gcttcgtcca ccaggtcgat ggctttgtct ggcaggaagc ggtcggtaat atagcg
//
LOCUS       DAORNF010000084         6446 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_8908_length_6446_cov_1.950555, whole genome shotgun sequence.
ACCESSION   DAORNF010000084 DAORNF010000000
VERSION     DAORNF010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 6446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6446
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8908_length_6446_cov_1.950555"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(181..987)
                     /locus_tag="PLV68_04765"
     CDS             complement(181..987)
                     /locus_tag="PLV68_04765"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRE00586.1"
                     /translation="MQLSGKHTVVTGAANGIGAALARRFHAEGASVVVADVDDAGAAG
                     VAAELDAIRPGSAVGVGVDLGTEAGNVALIRQAEESFGPIDLFFANAGVGIGSDPIET
                     DEAAWQLAFDVNVHAHRWAAKHLLPSWLERGEGYFCSTASAAGLLSQIGSAPYSVTKH
                     AAVAFAEWLSLTYGDRGLRVSCLCPQGVNTNMLALGDQPGAGISSSVVRSAGQVLEPN
                     EVADVAVEVIAAETFLIAPHPEVLTYWARKTADYDRWLAGMRKLQARMLG"
     gene            1133..1315
                     /locus_tag="PLV68_04770"
     CDS             1133..1315
                     /locus_tag="PLV68_04770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00587.1"
                     /translation="MTETNEAVITEALAVIDKALGEMLQRELVSSGEVADLLLDVRSL
                     LTHPAPAITAVSAVTA"
     gene            complement(1392..>2070)
                     /locus_tag="PLV68_04775"
     CDS             complement(1392..>2070)
                     /locus_tag="PLV68_04775"
                     /inference="COORDINATES: protein motif:HMM:NF018657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="WS/DGAT domain-containing protein"
                     /protein_id="HRE00588.1"
                     /translation="SDTDHAKSPLWTERSLRRSIETMRAPFGPTRDTARRLGGTLNTA
                     FLTIAAEAASRYHEQLGAPVEALRASMAISTRTEGSGANAFSLARMLIPTAPMPIADR
                     FAAVQAQAEAARGGSSAASLDTLAAVGAALPTSLITRLARSQSQTVDFATSNVKGSPI
                     PVYLAGARIMENYPVGPLGGVAFNLTLLSYAGSLDMGVTVDTAAVAEPERLRRCIEAS
                     VKAFIAS"
     assembly_gap    2071..2080
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2081..2809)
                     /locus_tag="PLV68_04780"
     CDS             complement(<2081..2809)
                     /locus_tag="PLV68_04780"
                     /inference="COORDINATES: protein motif:HMM:NF014998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0045017 - glycerolipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HRE00589.1"
                     /translation="MTDEQVLDPSAATPEFDTRMSDAEGLMWRLEKDPYLSSTVGNVT
                     ILDRAPDFERLVRRMEVATALVPRLRQRVHVAPANVSPPVWVDDSNFDIRFHVRHIAL
                     PKPGTTRQLFDLASLLIHDSFDRTRPLWQFTVVDGLRGGRSALIQKLHHTIADGEGLI
                     QVSLAFLDFDRDAPEIPMPDVPSPSAVNPPRTGTGQELLKGLVNNSLRMPRGIARHLK
                     GLLTDPTQIPSAGSSAAEAVRGVVT"
     gene            complement(2806..3378)
                     /locus_tag="PLV68_04785"
     CDS             complement(2806..3378)
                     /locus_tag="PLV68_04785"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HRE00590.1"
                     /translation="MATFTPHSVTGWRAAVDAFDEWADQALERVRGNRTADAAFSLAS
                     HLGDFSVLWHIVGTARGLTSDRRANQAIALSVVLGVESLVVNQGVKRLFRRARPTEAG
                     DERFPVRKPSTSSFPSGHASSAFTAAAVLTSWGGARTAPFWFGLAAVVGVSRAYVRIH
                     HASDIVGAVVGLAVGIAAARLLRRCRSLAA"
     gene            complement(3398..4213)
                     /locus_tag="PLV68_04790"
     CDS             complement(3398..4213)
                     /locus_tag="PLV68_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HRE00591.1"
                     /translation="MFPVPTSLSPSRVESFLSCPLAFRFASIEKLPEPPSVHTTRGSL
                     VHRALELLFVHPAEARTEDAALASLDQAIGEYRNHPDFTDLDLSETAQETFFTECRQL
                     VASYLRMEDPRSVRDIGLELRLEAPVGSLTLRGIIDRLELDEQGGLVVTDYKTGRAPS
                     PNYEQKSLSGVQFYAFLCHAVFGQRPAAVRLMYLRAGEIITATPTEQSVRFVTTRSKA
                     VLQAVEKACTTGQFLPRPSALCNGCSFKQWCPAFGGTPELAAVQVGQRTGAAA"
     gene            complement(4274..5146)
                     /locus_tag="PLV68_04795"
     CDS             complement(4274..5146)
                     /locus_tag="PLV68_04795"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRE00592.1"
                     /translation="MGSRRVGDIAVHELSHHAPEHGETTVLFAHATGFHAHCWLPVAA
                     HLPNVHSIGYDARGHGDTPIGPAWEAADSVDWTVYGADATAVATVVAAEAGAPLVGVG
                     HSMGGAALIMAALARPELFRALVVYEPIVIPPITDRDASFENPLASGARRRRATFPDV
                     ETAIANYASKPPMSAFADGVVDAYVRYGFGPALDDHGEPTGEITLKCRPEHEARTYEA
                     GGNHATWKRLAELQVPIWVLSGQTEPMQPSSWTETLAAQIPKARFVRLDDLRHFGPLE
                     APAAIVAEADAATR"
     gene            complement(5146..5430)
                     /locus_tag="PLV68_04800"
     CDS             complement(5146..5430)
                     /locus_tag="PLV68_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00593.1"
                     /translation="MSTDDGTRNADSRNNSAASGRSGPSPALIAFAVVAIVSVVLFFR
                     NGDPNTIDFLIFEWRTTVRFSILIAVALGIVLNRLFSIWWRRRRNKRNDR"
     gene            complement(5474..>5727)
                     /locus_tag="PLV68_04805"
     CDS             complement(5474..>5727)
                     /locus_tag="PLV68_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRE00594.1"
                     /translation="KRQGPQASTAASLMKLGITEMMFDTAMLRGDLAGAEAMLTGPDA
                     YGMLAAPGGRIAGGTSQVQRNIIGERLLGLPREPRGERA"
     assembly_gap    5728..5737
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5738..6437)
                     /locus_tag="PLV68_04810"
     CDS             complement(<5738..6437)
                     /locus_tag="PLV68_04810"
                     /inference="COORDINATES: protein motif:HMM:NF014789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRE00595.1"
                     /translation="MPRPTSCAFGGRGLTAAHQAAWIEACAIAGVPPFLNMVGLVLAG
                     QSIQRFGTPAQQAEHLAPTVRGERVWCQLFSEPEAGSDLGGLACKAELDGERFVINGQ
                     KVWCSGGRYSDWGILMARTDPAAAKHEGISFFLCPMDLPGIEIRPLRQMTGEAEFDEV
                     FFTDAELPAHHLLGPLHGGWGVGMAVLTNERGHIGASVIGLERRLESMAALAEGRRLD
                     PIERQRLAALLARGN"
BASE COUNT          996 a         2119 c         2310 g         1001 t
ORIGIN      
        1 cgcggcgaca cgtccgacca gccagtgcga ccgtggtgtc gggcgtgccc gggccggctc
       61 gccccgttgc agcccgacgc ggtcgagcgc caccgtcgtg gcgtgattgc tctggccagt
      121 gtggctgttc gggccggcgc ggtcgagcga gccggcgtca ccctcgggca tggttcggga
      181 tcagcccagc attcgggctt gcagcttgcg catgcctgcc aaccagcggt cgtagtcggc
      241 ggtcttgcgc gcccagtagg tgagtacctc gggatgcggc gcgatcagga aggtctcggc
      301 cgcgatgacc tcgaccgcca cgtcggcgac ctcgttgggt tccagcacct gcccggccga
      361 acgcacgacg ctgctggaga tgcccgcacc gggctggtcg ccgagggcga gcatgttggt
      421 gttgacccct tgcggacaca ggcagctgac ccgcagcccc cggtcgccgt aggtcaggct
      481 gagccactcg gcaaacgcga cggcggcgtg cttggtgacg ctgtagggcg cggagccgat
      541 ctgactgagc agccccgcgg ctgaagcggt ggagcagaag tagccctcgc cccgttcgag
      601 ccagctcggt aacaggtgtt tggcggccca ccgatgagcg tggacgttga cgtcgaacgc
      661 gagttgccac gccgcctcgt cggtctcgat cgggtcgctg ccgatgccga cgcctgcgtt
      721 ggcgaagaac aggtcgatcg gcccgaagct ctcctcggct tggcggatca gagcgacgtt
      781 gcccgcctcg gtgccaaggt cgactccgac tcccaccgcc gagccaggac gaatcgcgtc
      841 gagctctgcg gcaaccccag cggctcccgc atcgtcgacg tcggcgacca ccacagatgc
      901 gccctcggcg tggaaccgtc tggccaacgc cgcaccgatg ccattggctg cgccggtgac
      961 gaccgtgtgt ttcccagaga gttgcatggc cagagcgtac gtgagccacg tcgactccac
     1021 ggcgagcgcg cgcgagacaa gacgcgatgc tgccgttacc atgcatcatc agcacggcgc
     1081 accgggatac ggtgaccgca acaccgtgcc aaccgccgga aggagcccgt cgatgaccga
     1141 gaccaacgag gcagtgatca ccgaagcgct ggccgtcatc gacaaggcgc tcggcgagat
     1201 gttgcagcgt gagttggtgt cgtcgggcga agtggccgac ctgttgctcg acgtccgttc
     1261 gctgctcacc caccccgcgc cagccatcac cgcggtctcc gctgtcacag cctgatctga
     1321 gtccgcgtcg gcgcgtcaac cgggcaatgc cccggtcggc gggccaggcc acgtcaactg
     1381 gcgatggttg atcaggacgc gatgaaggcc ttgaccgagg cctcgatgca acgccggagc
     1441 cgctcgggtt cggcgaccgc ggccgtgtcg acggtgacgc ccatgtcgag gctgcccgcg
     1501 tagctgagca gcgtcaggtt gaacgccacc ccgccgagtg gtccgaccgg gtagttctcc
     1561 atgatccgcg ccccggcgag gtagacgggg atcggtgatc ccttgacgtt gctggtggcg
     1621 aagtcgaccg tctggctctg gctgcgggcc aggcgggtga tcagcgaggt cgggagcgcg
     1681 gcaccgaccg cggccaacgt gtcgagtgaa gccgccgacg agcccccccg agctgcctcg
     1741 gcttgggcct gcaccgcggc gaagcggtcg gcgatgggca tcggcgcggt ggggatcagc
     1801 atgcgcgcca gcgagaacgc attggcgccg gacccttcgg tgcgggtact gatcgccatc
     1861 gacgcgcgga gcgcttcgac cggggcaccc aactgctcgt gatagcggct ggctgcctcg
     1921 gcagcgatgg tcaagaatgc cgtgttcagt gtgccaccca gccgtcgggc ggtgtcgcgg
     1981 gtgggaccga acggcgcccg catcgtctcg atgctgcggc gcagcgagcg ctcggtccac
     2041 agcggcgact tggcgtggtc cgtgtcgctc nnnnnnnnnn ggtgacgaca ccgcgcactg
     2101 cctcggccgc cgacgaacca gccgacggga tctgggtcgg atcggtgagc agtcccttga
     2161 gatggcgagc gatgccgcgg ggcatgcgca ggctgttgtt gaccaacccc ttgagcagct
     2221 cctggcccgt gccggtccgt ggcgggttca ctgcgctggg cgagggcacg tcgggcatcg
     2281 ggatctcggg tgcatcacgg tcgaagtcga gaaacgccag gctgacctgg atcagacctt
     2341 caccgtcggc gatggtgtga tgcagcttct ggatcagcgc ggagcgtccg ccgcgcaggc
     2401 cgtcgaccac ggtgaactgc cacagcggcc gagtgcgatc gaacgaatcg tggatcagca
     2461 gcgaggccaa atcgaacaat tgtcgcgtgg tgccgggctt gggcagcgcg atgtggcgaa
     2521 cgtggaagcg gatgtcgaag ttgctgtcgt cgacccacac cggcgggctg acgttggccg
     2581 gtgcgacgtg aacccgctgg cgcagccgtg gtaccagcgc cgtggcgacc tccatccgcc
     2641 ggaccagccg ttcgaagtcg ggtgcccgat cgaggatggt gacattgccg accgtggacg
     2701 acaggtaggg gtccttctcc aggcgccaca tcagaccttc ggcatcggac atgcgggtgt
     2761 cgaactccgg tgtcgcggcc gacgggtcga ggacctgctc gtctgtcatg cggcgagact
     2821 acgacagcga cgaagcaggc gtgctgccgc gatgccgacg gccaggccga ccaccgcgcc
     2881 gacgatgtcg ctggcgtgat ggatgcgcac gtaggcccgg ctgacgccga ccaccgccgc
     2941 gaggccgaac cagaacggag ccgtgcgggc cccgccccag gatgtgagca cggcggctgc
     3001 ggtgaacgcc gacgaggcat gcccgctggg aaagctggac gtggagggct tgcgcaccgg
     3061 gaagcgctcg tcgccagcct cggtcggccg ggcgcggcgg aacagacgct tgacgccttg
     3121 attgaccacc aggctctcga cgccgagcac gaccgacagc gcgatcgcct ggttggcgcg
     3181 gcggtcgctg gtcagcccgc gcgcggtgcc gacgatgtgc cacaacacgc tgaagtcgcc
     3241 gagatggctg gccaggctga acgcagcatc ggcggtgcgg ttgccgcgga cgcgttcgag
     3301 tgcctggtcg gcccactcgt cgaacgcgtc gaccgcggcc cgccagccgg tgacggagtg
     3361 gggtgtgaac gtcgccatgg tcgtggtcgg tgggtgttca ggccgcggcg ccggtgcgtt
     3421 ggccgacttg caccgccgcc agttcgggcg tgccgccaaa ggcggggcac cactgtttga
     3481 acgaacagcc gttgcacaac gcgctgggcc ggggcaggaa ctgaccggtg gtgcaggcct
     3541 tctcgaccgc ttgcagcaca gccttgctgc gggtggtgac gaagcgcacc gattgctcgg
     3601 tgggcgtggc ggtgatgatc tcgcccgcgc gcagatacat caaccgcaca gcggctgggc
     3661 gctgcccgaa cacggcgtgg cacaagaacg cgtagaactg caccccgctg aggctcttct
     3721 gctcgtagtt ggggctgggg gcgcgacccg tcttgtagtc ggtgaccacc aggccgccct
     3781 gttcgtcgag ttccaaccgg tcgatgatgc cgcgcagcgt caacgaaccg accggcgcct
     3841 cgaggcgtag ttccaggccg atgtcgcgca cggagcgtgg gtcttccatg cgcaggtacg
     3901 acgcgaccaa ctggcggcac tcggtgaaga acgtctcctg cgcggtttcg ctgaggtcga
     3961 gatcggtgaa gtcggggtga ttgcggtact cgccgatcgc ctggtcgagc gaggcgaggg
     4021 ctgcatcttc ggtgcgcgcc tcggcggggt gcacgaacag cagctcgagc gcccggtgca
     4081 ccaacgagcc gcgggtggtg tgcacgctgg gtggctcggg caatttctcg atgctggcga
     4141 agcggaacgc gagcggacac gacaagaacg attcgacccg gctgggcgac aagctggtgg
     4201 gaaccgggaa catgcaggcc atggtagggg aggggtgcgc gctggttccg gacccgacgc
     4261 ggcgctcgcg gcgctatcgc gtcgcggcgt cggcttcggc gacgatcgcg gcaggcgcct
     4321 ccagcggtcc gaagtgacgg aggtcgtcga gccgcacgaa gcgcgctttg gggatctgcg
     4381 ccgccagcgt ctcggtccac gacgatggct gcatcggttc ggtctggccg ctgagcaccc
     4441 agatcggcac ctgcaactcg gccaggcgtt tccaggtggc gtggttgccg ccggcctcgt
     4501 aggtgcgtgc ctcgtgttcg ggccggcact tcagggtgat ctcgccggtt ggttcgccgt
     4561 ggtcgtcgag cgctgggccg aacccgtagc gcacgtaggc gtcgaccacc ccgtcggcga
     4621 aggcgctcat cggcggcttg ctggcgtagt tggcgatcgc ggtctcgaca tcggggaacg
     4681 tcgcccggcg acggcgggca ccggaggcga gcgggttctc gaacgaagcg tcgcgatcgg
     4741 tgatcggggg gatcacgatc ggctcgtaca cgaccagggc ccggaacaac tcgggccggg
     4801 cgagcgcggc catgatcagt gctgcgcctc ccatgctgtg accgacgccg accagtggcg
     4861 caccagcctc ggcggccacc acggtggcga cggcggttgc gtcggcccca tagaccgtcc
     4921 agtcgaccga atcggccgcc tcccatgcgg ggccgatcgg cgtgtcgccg tggccgcggg
     4981 cgtcgtagcc gatgctgtgg acgttgggca ggtgggctgc gaccggcaac cagcagtggg
     5041 cgtggaaccc ggttgcgtgg gcgaagagca ccgtggtctc accatgctca ggggcgtggt
     5101 gagacagctc gtggacggcg atgtcgccga cgcggcggga ccccatcaac gatcgttgcg
     5161 cttgttgcga cggcggcgcc accagatgga gaacagccgg ttgagcacga tgcccaacgc
     5221 gaccgcgatc aggatcgaga agcgcaccgt ggtccgccac tcgaagatca agaagtcgat
     5281 cgtattcggg tcgccgttgc ggaagaacag cacgaccgac acgatcgcga cgactgcgaa
     5341 cgcgatcagc gcgggacttg gccccgaccg tccactcgcc gcgctgttgt tgcggctgtc
     5401 ggcgttgcga gtgccgtcgt cggtgctcat gattggcctc ccgaaatctc ggtcgtggtg
     5461 gggtcgaggt tcatcatgcc cgttcccccc ttggctcgcg cggcaaacca aggaggcgtt
     5521 ccccgatgat gttgcgctgc acctggctgg tcccgccggc gatgcgcccg ccgggtgccg
     5581 cgagcatgcc gtaggcatcg ggtccggtca gcatcgcctc ggccccggcc aagtcgccgc
     5641 gcaacatcgc ggtgtcgaac atcatctcgg tgatgcccag cttcatcagt gaagcagccg
     5701 tgctggcctg gggaccctgg cgcttgcnnn nnnnnnnagt tgccccgcgc cagcagcgcc
     5761 gccagccgtt gccgctcgat cgggtcgagg cgacgaccct cggccagcgc agccatcgat
     5821 tcgagccgcc gttccaggcc gatcacgctg gccccgatgt ggccacgttc gttggtgagc
     5881 acggccatgc ccacgcccca cccgccgtgc aacggtccga gcagatggtg ggcgggcagt
     5941 tcggcgtcgg tgaagaacac ctcgtcgaac tcggcctcgc cagtcatctg gcgcagcggg
     6001 cggatctcga tgccgggcag gtccatggga cacaagaaga acgagatgcc ctcgtgcttg
     6061 gccgcggccg ggtcggtgcg ggccatcagg atgccccagt cgctgtaacg cccgccgctg
     6121 caccacacct tctgaccgtt gatgacgaac cgttcaccgt cgagctcggc cttgcacgcc
     6181 agcccaccca ggtcgctacc cgcctcgggc tcgctgaaca gttggcacca cacccgctcg
     6241 ccgcgcacgg tcggggcgag gtgctcggct tgctgggccg gtgtgccgaa gcgttggatc
     6301 gactgaccgg ccaacacgag cccgaccatg ttcagaaacg gtgggacgcc agcgatcgcg
     6361 caggcctcga tccatgcggc ctggtgagcc gccgtcagcc cgcgcccgcc gaaggcgcag
     6421 ctcgtcggcc gcggcactgg caggcg
//
LOCUS       DAORNF010000085         6418 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_8957_length_6418_cov_1.806852, whole genome shotgun sequence.
ACCESSION   DAORNF010000085 DAORNF010000000
VERSION     DAORNF010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 6418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6418
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8957_length_6418_cov_1.806852"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(23..>360)
                     /locus_tag="PLV68_04815"
     CDS             complement(23..>360)
                     /locus_tag="PLV68_04815"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HRE00596.1"
                     /translation="LNPGTSRSGITISAARFFGFDRDTAARISFLMSLPIVAGAVVFK
                     VAGLIADGIPDGLLAPMVVGVIASAISGWVAVAGMLALIRTRSFTPFVLYRVALGVVV
                     LVVVAAGWR"
     assembly_gap    361..606
                     /estimated_length=246
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<607..855)
                     /locus_tag="PLV68_04820"
     CDS             complement(<607..855)
                     /locus_tag="PLV68_04820"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HRE00597.1"
                     /translation="MPILHAIVLGLVQGLSEFLPISSSGHLLLVPWFFGWDDLPDAAT
                     KKAFDVALHLGTFVAVVGYFRRDIARYVRAGTRVVVKRD"
     assembly_gap    1035..1110
                     /estimated_length=76
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1111..1598)
                     /locus_tag="PLV68_04825"
     CDS             complement(<1111..1598)
                     /locus_tag="PLV68_04825"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HRE00598.1"
                     /translation="MPRPSPAAAARHPHLADPLPAGWVRVDLHSHTMWSGDSTTTPDE
                     IEAAVVEAGLDVVCITDHNAIKGALDLGDRLPCRVIVGEELKTQAGEIIGLFLTERIV
                     AGTPPVEAAQAIRAQGGVVYVPHPFDPMRRNLAEAPLRDLAGRGLIDAVEVINAKTSL
                     ASL"
     gene            1806..>2008
                     /locus_tag="PLV68_04830"
     CDS             1806..>2008
                     /locus_tag="PLV68_04830"
                     /inference="COORDINATES: protein motif:HMM:TIGR01439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HRE00599.1"
                     /translation="MVTIDKAGRVVIPKEVRDRLDLVPGTELDVEISRDAIVLVPERR
                     TAARFEVIDGVAVFPAVSGVELTP"
     assembly_gap    2009..2083
                     /estimated_length=75
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2084..2479
                     /locus_tag="PLV68_04835"
     CDS             <2084..2479
                     /locus_tag="PLV68_04835"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HRE00600.1"
                     /translation="DTSVAVAAMQAGHSMHAACVAAVRRYRPALAGHAAYETFSVLTR
                     LPGDAKLGATTARAAIRATFHDVVWLTGKQSAALLNRLAVVGITGGGVYDALVGEAAR
                     VNGHTLLTCDARARTTYDVLGVPYLLVAS"
     gene            complement(2483..3832)
                     /locus_tag="PLV68_04840"
     CDS             complement(2483..3832)
                     /locus_tag="PLV68_04840"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HRE00601.1"
                     /translation="MTQAAELMAIADRIVAQAAAGEQIEAYVSRGSETDIRVYNGEVE
                     HFVSAQAEGVGIRVIRDGRTGFAYAGTLDADAVAAVLADARDNVVFGTPDEWAGLVEP
                     DGVAVIEQQLWSDELAAYDTAAKIELAKELERLTLAADRRVRTDDANYSDASGEAAVA
                     TTTGVRVSGRENGCYASVSTLADDGDETQTGFGFAVGRSPYEFDLAKAAREAAERATR
                     LLGAVKPPSKKITVVLDPMVTAQFLGVLSSALNGENVAKGRSLFAGRVGEQVASPLFT
                     LIDDPTNPKAYTATDIDGEGLAARRNVLVEGGVLRGFVHSSYSARRAGTVSTANAVRG
                     GFRGTPGVGCLAMQLQPGTRDQAALIADVADGVLIQSVQGMHSGVNPISGDFSTGASG
                     LLISNGTLGGPVREFTIASTLQKMLHDIVEVGGDLYWLPMRATGLSLVIADVTMSGA"
     gene            complement(3829..>5006)
                     /locus_tag="PLV68_04845"
     CDS             complement(3829..>5006)
                     /locus_tag="PLV68_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HRE00602.1"
                     /translation="ERGLLAAAQAAAAAAAQGGGGARTVALSHSVPPQVNTVGIYPDS
                     IGKAAKVALLTRIDEAARATGDAIVQVSAGYSDSRKRVLIANSDGLFTDDEQVRHLVR
                     INVVANGDTGMQTGFESLGHTIGFEIFDEHDVEELARRAATQALTKLRARPAPSGAVP
                     VVIKAGSGGVLFHEACGHGLEADLVSKGASVYQGKLGEQVASPLVTLVDDGTMSGEWG
                     AIAIDDEGHPSQYNVLIQNGVLTDYMWDHLRARKQGHAPSGNGRRQSYMHLPMVRMTN
                     TYVLNGSEDPDEIVRDTPHGVYVAKLGGGSVNTATGDFVFGMTEAYLIENGEITEPLR
                     QGNLIGNGPQVLRDIERLGNDFAMGNPGTCGKDGQGVPVGDGQPTLRVKSMTIGGTA"
     assembly_gap    5007..5174
                     /estimated_length=168
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5315..5698
                     /locus_tag="PLV68_04850"
     CDS             5315..5698
                     /locus_tag="PLV68_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012231502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HRE00603.1"
                     /translation="MGIAIDTGLRGEVTRVVSDDDTAIAYGSGSVPVLSTPCVVTLCE
                     QAAVAAVADRLPAGVTTVGMRVQVDHLRPTSVGRSITAEAVLRHVEGRRLTFAVSVDD
                     DRGLVAAGKVTRVIVEIERFMDKAT"
     gene            5831..>6097
                     /locus_tag="PLV68_04855"
     CDS             5831..>6097
                     /locus_tag="PLV68_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00604.1"
                     /translation="MATALTAILASAALIGACSSDDKASGNGSAFNGSDGKGSGSAAT
                     STTVAPQPTSVFDLARSTTCTADRQTLELAVEAFRELAVGEGLVQ"
     assembly_gap    6098..6107
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          888 a         2050 c         1975 g          930 t
ORIGIN      
        1 gcccgccgaa cgctaccagc gctcagcgcc agccggctgc gacgacgacc agcaccacga
       61 caccgagcgc cacgcggtac aggacgaagg gcgtgaagct gcgggtgcgg atcagggcga
      121 gcatgccagc cacggcaacc caaccgctga tggccgaagc gatcaccccg accaccatcg
      181 gagccagcag gccgtcggga atgccgtcgg cgatcaaccc ggccaccttg aacacgaccg
      241 caccggcaac gatcggcagt gacatgagga agctgatccg cgccgcggtg tcacggtcga
      301 agccgaagaa ccgcgcggcc gaaatcgtga tgcccgaccg tgacgtgccc gggttgaggg
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      601 nnnnnngtcc cgcttgacca ccacccgggt gcccgcccgc acgtagcgcg cgatgtcgcg
      661 gcggaagtac ccgacgaccg cgacgaaggt gccgaggtgc agggcgacgt cgaacgcctt
      721 cttggtcgcc gcgtcgggca gatcgtccca gccgaagaac cagggcacca acagtaggtg
      781 accgctggac gagatgggca agaactcgga cagcccctgc accaggccga ggacgatggc
      841 gtgcaggatc ggcatcggca ctgtctatcc ggccgctctc gcaatctcgc gtcaggacgc
      901 ggtggtgccg ggcacgatgc gcggggtcca cggacggggc tggtcccagt ggtggccgac
      961 cgcgcgaccc agagccagct tggccaggaa gtcggccggg ccgtcgaagt cgggcatctc
     1021 gacgtacgcg gcgcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn agcgaggcca gcgacgtctt ggcgttgatc
     1141 acctcgaccg cgtcgatcag accgcgtccg gccaggtcac gcaacggcgc ctcggccagg
     1201 ttgcgccgca tcgggtcgaa cgggtgagga acgtagacca cgccaccctg cgcccggatc
     1261 gcctgcgccg cctcgaccgg cggcgtgccg gccacgatcc gctcggtgag gaacaacccg
     1321 atgatctctc cggcctgggt cttcaactcc tcgccgacga tcacccgaca tggcagccga
     1381 tcgcccagat cgagcgcacc cttgatcgcg ttgtggtcgg tgatgcacac cacgtcgaga
     1441 cccgcttcga ccacggcggc ttcgatctcg tccggggtcg tggtcgagtc gccgctccac
     1501 atcgtgtggc tgtgcagatc gacccgcacc caacccgccg gcagcgggtc ggcgagatga
     1561 gggtgacgcg cagcagcagc cggggacgga cgaggcaccg cgtcaacgta gcggggtcag
     1621 ggtttggtgc tcacatgacg gtgcgttgga acttgcgcat caggtgggga ccggcgagca
     1681 cggggtcgtg ctttgccggg ccgaggcacg ctatgtcacg gctacccgcg aggcggcctc
     1741 cgggtgacgc ggggacagct gcgcggaaat ctcattcctc gcgcaatggt acgatggcat
     1801 ccgtgatggt aacgatcgac aaggcggggc gggtggtgat ccccaaggag gtccgcgatc
     1861 gtctcgacct ggtgccgggc accgagctcg acgtcgagat cagccgtgat gccatcgtcc
     1921 tcgtgccaga gcggcgcacc gctgcccgct tcgaggtcat cgacggggtt gccgtctttc
     1981 ctgccgtgtc cggcgtggag ttgacaccnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngacacca gcgtcgccgt
     2101 cgccgcgatg caggcgggac actcgatgca cgcggcctgt gtcgccgcgg ttcggcgtta
     2161 ccggccagcg ctcgccgggc acgcagcgta cgagaccttt tcggtgctga cccgcctccc
     2221 gggtgacgcc aagctcggag cgaccacggc gcgcgcagcg atccgggcca cgttccacga
     2281 cgtggtgtgg ctgacaggca agcagtccgc cgccctcctc aaccggctcg cggtcgtcgg
     2341 tatcacggga ggcggggtct acgacgcgtt ggtcggtgag gccgcgcggg tgaacggcca
     2401 cacgttgttg acctgcgatg cgcgtgcccg aacgacgtac gacgtgctcg gggtgccgta
     2461 cctgctcgtc gcctcgtgat ctctacgcgc cggacatggt gacgtcggcg atgaccaggc
     2521 tgaggccggt ggcccgcatg ggcagccagt agaggtcgcc gccgacttcg acgatgtcgt
     2581 gcagcatctt ctgcagggtg gacgcgatgg tgaactcgcg caccgggccg ccgagggtgc
     2641 cgttgctgat caacagaccc gatgctcccg tggagaagtc gccgctgatc gggttgacgc
     2701 cgctgtgcat gccctgcacg ctctggatca gcacgccgtc ggcgacgtcg gcgatcagcg
     2761 cagcctgatc gcgtgtgccc ggctgcaact gcatcgccag gcagccgacg cccggtgtgc
     2821 cacggaagcc gccgcggacc gcgttggccg tgctgaccgt gcccgcccgt cgcgcgctgt
     2881 agctggagtg gacaaaccca cgcagcaccc cgccttcgac cagcacgttg cgccgcgcgg
     2941 cgagaccctc accgtcgatg tcggtcgcgg tgtacgcctt ggggttggtc gggtcgtcga
     3001 tcagcgtgaa cagcggcgag gcgacctgtt cgccgacccg tcccgcgaac agactgcgac
     3061 ccttggccac gttctcgccg ttgagcgcgc tggacagcac acccaggaac tgggcggtga
     3121 ccattgggtc gagcacgacc gtgatcttct tcgacggcgg cttgaccgca ccgagcaggc
     3181 gggtcgcccg ttcggccgcc tcgcgggcgg ccttggccag atcgaactcg tacggcgagc
     3241 gaccgaccgc aaagccgaac ccggtctggg tctcgtcgcc gtcgtcggcc agcgtcgaca
     3301 cgctcgcgta gcaaccgttc tcgcgcccgc tgacgcggac accggtggtg gtcgccaccg
     3361 cggcctcgcc cgaggcgtcg ctgtagttgg cgtcgtcggt gcggacgcgc cggtcagcgg
     3421 caagcgtcag gcgctccagt tccttggcca gctcgatctt ggcggccgtg tcgtaggcag
     3481 cgagctcgtc gctccacaac tgctgctcga tcaccgccac gccgtcgggc tcgaccaacc
     3541 cggcccactc gtcgggcgtg ccgaacacca cgttgtcgcg cgcgtcggcc aacacggctg
     3601 cgaccgcgtc ggcgtcgagg gtgcccgcgt acgcgaagcc ggtccgaccg tcgcggatca
     3661 cgcggatgcc gaccccttcg gcctgggcgc tgacgaagtg ctcgacctcg ccgttgtaga
     3721 cccggatgtc ggtctcgctc ccccgcgaca cgtaggcctc gatctgctca cccgccgcgg
     3781 cctgggccac gatccgatcc gcgatcgcca tcaactcggc tgcctgtgtc acgcggtccc
     3841 gccgatggtc atcgacttga cgcggagggt gggttggccg tcgccgacgg ggacgccttg
     3901 gccgtccttg ccgcaggtgc cggggtttcc cattgcgaag tcgttgccga gccgctcgat
     3961 gtcgcgcaac acctgcggcc cgttgccgat caggttgccc tgccgcaacg gctcggtgat
     4021 ctcaccgttc tcgatcaaat aggcctcggt catgccgaac acgaagtcgc ccgtggcggt
     4081 gttgacactg cccccgccga gcttggccac gtacacaccg tgtggcgtgt cgcgcacgat
     4141 ctcgtccggg tcctcgctgc cgttgagcac gtaggtgttg gtcatccgca ccatcggcag
     4201 gtgcatgtag ctctgccgcc gcccgttgcc gctgggggcg tggccctgct tgcgagcgcg
     4261 gaggtgatcc cacatgtagt cggtcagtac gccgttctgg atcagcacgt tgtactggct
     4321 gggatggccc tcgtcgtcga tcgcgatcgc gccccactca ccgctcatcg tgccgtcgtc
     4381 gaccagcgtc accagcggcg aggccacctg ctcgccgagc ttgccctggt acacgctggc
     4441 gcccttggac accaggtcgg cttcgaggcc gtggccacac gcctcgtgga acaacacgcc
     4501 gccgctgcct gccttgatca ccacggggac cgcgcccgac ggcgcgggac gggcgcgcag
     4561 cttggtcagc gcctgggtcg cagcgcgccg ggccagttct tcgacgtcgt gttcgtcgaa
     4621 gatctcgaac ccgatcgtgt gccccaacga ctcgaacccg gtctgcatgc cggtgtcgcc
     4681 gttggcgacc acgttgatgc ggacgaggtg acggacctgc tcgtcgtcgg tgaacagccc
     4741 gtcgctgttg gcgatcagca cccgcttgcg gctatcggag tacccggccg acacctgcac
     4801 gatcgcgtcg cccgtggccc gggccgcctc gtcgatccgc gtcagcagag caaccttggc
     4861 cgccttgccg atcgagtcgg gatagatccc gaccgtgttg acctgcggcg gcaccgaatg
     4921 cgacagcgcg acggtgcggg cacccccacc gccctgggcg gctgccgcgg cggcggcctg
     4981 ggcggctgcg agcaaccctc gttcggnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5161 nnnnnnnnnn nnnngttcgc aacgccgcac cgagcacccg ctcgagaacg tcttgatcga
     5221 tcacccgact cacgctactg cggccccctc agatgccacc tcgccccgtc gccggaggcc
     5281 agtcccgacc ttcgagtaga tagcgtgctc cgccatgggt atcgccatcg acaccggact
     5341 gcgcggagag gtcacccgcg tcgtgtccga cgacgacacc gctatcgcct acggatcggg
     5401 cagcgtgccg gtgttgagca cgccctgcgt cgtcaccctg tgcgagcagg cggccgtcgc
     5461 cgccgtcgcc gatcggctcc ccgcgggggt caccacggtc ggcatgcggg tgcaggtcga
     5521 ccatctccgc ccgacctcgg tcggtcgcag catcaccgcc gaagcggtcc tccgtcacgt
     5581 cgaaggacgg cggctcacgt tcgcggtgtc ggtcgacgac gatcgtggtc tggtcgcagc
     5641 gggcaaggtc acccgggtga tcgtcgagat cgagcgattc atggacaagg ccacctgaac
     5701 cgataacgac ggcatgtcca acccgccgtc accgtctcct tcattgtcgg caccttcatt
     5761 ctcggcacct tcatcgtcgg catcatcggc atcgtcggcg tcactgcgcc gcggtcgtcg
     5821 gccagcggcc ttggccactg ccctcacggc gatcctcgcc agcgcagcgt tgatcggcgc
     5881 atgcagcagc gacgacaagg ccagtggcaa cggttcggcc ttcaacggtt ctgacggcaa
     5941 gggttctggc agcgcagcga cgtccaccac ggtcgcgccg caaccgacca gcgtgttcga
     6001 cttggcccga tccacgacct gcaccgccga ccgccagacg ctggaactcg cggtcgaggc
     6061 cttccgtgaa cttgcggtcg gagagggcct tgtacagnnn nnnnnnngag gccttccggg
     6121 cgatcaacga gtcggtgcct gacgaagccg acctggtcgc gacgggcttc ctgcgcgagg
     6181 agagcgcagg gctcgacgtg gccgcggacg gatcgatcgt cgccgcgccc ggcggcccgt
     6241 gcgccggatg atcagcccgt agactcgccc ctcgtgccat cgcggaggga tcgtgtgctg
     6301 tccaagaccc gcacggcgct tccgtggata cgtgaaatcg cagtcattgc cgtcgtctac
     6361 gtggtgtacg gcttggtgcg caaccagttc gggtcggcca ccctcatgcc gggtgacg
//
LOCUS       DAORNF010000086         6369 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_9037_length_6369_cov_1.459930, whole genome shotgun sequence.
ACCESSION   DAORNF010000086 DAORNF010000000
VERSION     DAORNF010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 6369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6369
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9037_length_6369_cov_1.459930"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..>439)
                     /locus_tag="PLV68_04860"
     CDS             complement(64..>439)
                     /locus_tag="PLV68_04860"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRE00605.1"
                     /translation="FQLYSSGTTGHPKGVMLSNDNFFGLLPLAKENWELHPEAVNLAA
                     MPLFHIGGGGWAVAGMYEGATTVIVRDLDPAALITLIGTSTGFSVSGGAEGVGRATTR
                     AVVLSISAIVVADMLFSFFLNR"
     assembly_gap    440..467
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<468..>753)
                     /locus_tag="PLV68_04865"
     CDS             complement(<468..>753)
                     /locus_tag="PLV68_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRE00606.1"
                     /translation="FLDKNGIEHFEVFYGASLLNAVCVDVNWRLAAPEVEFIVNDAQA
                     KVLVVGPDFVPILDAIAPNLTTVTKILVIGGHERFDDYGAWVDAQEPVDPG"
     assembly_gap    754..966
                     /estimated_length=213
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1072..>2636
                     /locus_tag="PLV68_04870"
     CDS             1072..>2636
                     /locus_tag="PLV68_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRE00607.1"
                     /translation="MTATDVPGWNFAEIWEHHADRFPDALAQIHGDRTFTWAEFDRRA
                     DGIAATLLAPGVRHQDKVAHYLYNCPEYLESMFGMFKAGLVPVNTNYRYNDDEIVYLW
                     DNADAVAAVFHGAFAERCAALRDRLPGVRTWIWVDDGSGPCPDFAVPYETAAASATER
                     IVAPWGRSGDDIYMLYTGGTTGMPKGVMWRQRDLFGSLDASSRTQLPDTPDWDALDAR
                     VSKPGPRNLPAAPLMHGTGAFNAMWNLALAGAIVTMEGRHFDPIELLDTVQRMSIVGD
                     AFAKPVLRSLDAEPDRWDFSSMRVIISSGVMWSAATKSGLLAHNDRLIMVDSLGSSEA
                     LGMANNTTSAQGAGANATFVLGPNAKVLTDDGREVVPGSGERGRVALRGYMPLGYYKD
                     PVKSASTFIEFEGTRWSIPGDFAEIDADGTVRLLGRGSQCINTGGEKVYPEEVEEVLK
                     MHPSVHDAAVVGLADERFGETVNALVEPNPGAAIDEATLISFVKEHLAAYKAPKRVFD
                     VTSVNRAPNGKLD"
     assembly_gap    2637..2752
                     /estimated_length=116
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2827..>3283)
                     /locus_tag="PLV68_04875"
     CDS             complement(2827..>3283)
                     /locus_tag="PLV68_04875"
                     /inference="COORDINATES: protein motif:HMM:NF014517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidohydrolase family
                     protein"
                     /protein_id="HRE00608.1"
                     /translation="MESVVLQLLRERGHTLGVAESVTGGLVAGRITNVAGSSEVFRGG
                     VVAYDSQVKFSVLGVEPGPVVNEQTAAQMAVGVKAVVGSTVGLALTGVAGPAEQDGRA
                     VGTLCIGASVGDEVFTATVRLPGTRDQMRQMSVITALDFLRRRLGAQQG"
     assembly_gap    3284..3293
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3294..>3708)
                     /locus_tag="PLV68_04880"
     CDS             complement(<3294..>3708)
                     /locus_tag="PLV68_04880"
                     /inference="COORDINATES: protein motif:HMM:NF037737.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00609.1"
                     /translation="NARGTAPGLICPIMVDGRAKVLYALPGVPHEMYEMFTQTVLADL
                     HDRSGSDDVIVSRVLRTWGESESGLNERLDAVIAELDDIANPTLAFLASGWEGLKIRL
                     TAKAGTEEEALELLDRWTRRVTAVVGEFVFGFNDDT"
     assembly_gap    3709..3718
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3719..4098)
                     /locus_tag="PLV68_04885"
     CDS             complement(<3719..4098)
                     /locus_tag="PLV68_04885"
                     /inference="COORDINATES: protein motif:HMM:NF013183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HRE00610.1"
                     /translation="MRVDVVAVGTELLLGQIVDTNSAWIGEHLAAHGFDSLSQVKVGD
                     NLSRIVDALRLTLVGADAVVVCGGLGPTHDDLTREALAAIMGADLVSHDDIVEHIRSL
                     FAARNRTMAANNLRQALVPQGATV"
     gene            complement(4119..4706)
                     /locus_tag="PLV68_04890"
     CDS             complement(4119..4706)
                     /locus_tag="PLV68_04890"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HRE00611.1"
                     /translation="MTVDPTRIATWANLVTVARMFISPVVFIAFPDDGKGSWLALLVW
                     FLLCFSDGIDGFLARRHGPTNFGTFVDPLADKLLVLLAMITLIDSGQFWWVPVAIIFA
                     RELAMSLYRVFWGTKGVNVPASKLAKIKTTTQQFAVAFAIAPLTAVDAMWTWKVLLWT
                     SVVLTVYSFIQYMWRAHRAKEAGLDDRPVDHPASA"
     gene            complement(4703..>5488)
                     /locus_tag="PLV68_04895"
     CDS             complement(4703..>5488)
                     /locus_tag="PLV68_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRE00612.1"
                     /translation="QLDVREIVLIAQDLASYGKDRPDELGAGSIVALIEAVAARVDRT
                     RLLYLYPSDLTDALVDAICDTGVPYFDLSLQHVSKPLLRRMRRWGDGDRFLARIDDIR
                     KREPEAAFRSNFIVGYPGETEEDHDRLLDFVEAAQLDWCGFFAYSPEEGTYAMGLDGA
                     VDEGLMHERLAELREMQDSITAERRDSLIGRTVRVLVDEQGVGRTHREAPEIDGVVHV
                     ADGLAAGTFHDVLVVNAFGPDLVAAGAAGQTAEQMDDATERGR"
     assembly_gap    5489..5597
                     /estimated_length=109
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5598..>5933)
                     /locus_tag="PLV68_04900"
     CDS             complement(<5598..>5933)
                     /locus_tag="PLV68_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00613.1"
                     /translation="AAVDNFRIHVEGKGGHAARPHQSIDTILALDDQRASGSRLVVTG
                     CMAERYGAELAEALPEVDQVAGFGVSVNVLAPARKLIPVSSAPVPTLDLLNLPRPKSA
                     VPWAYVKVA"
     assembly_gap    5934..6066
                     /estimated_length=133
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6110..>6369)
                     /locus_tag="PLV68_04905"
     CDS             complement(6110..>6369)
                     /locus_tag="PLV68_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HRE00614.1"
                     /translation="LQLLDSLAVDGEGNVCVATLVNGGVTIISPDGQRIEHVPTDDPL
                     TTNICFGDDGSGEFRDAYITLSGSGRLVKLRWPYRGLALNY"
BASE COUNT          990 a         1973 c         1903 g          884 t
ORIGIN      
        1 gctcacgaca gcctgcagcc caccggctgc caattccgaa tccccaaacc cgaatcccgg
       61 ctctcatcga ttgaggaaga acgaaaacag catgtccgcc accacgatcg cgctaatcga
      121 cagcacgacg gcgcgggtgg tcgcacggcc gacgccttcg gcgccgccgc tgacggagaa
      181 ccctgtgctg gtgccgatca gggtgatcag cgcggccggg tcgaggtcgc ggacgatcac
      241 cgtggtcgcg ccctcgtaca tgcccgccac ggcccagccg ccaccaccga tgtggaacaa
      301 cggcatcgcg gccaggttga ccgcctcggg gtgcagctcc cagttctcct tggccagcgg
      361 caacaagccg aagaagttgt cgttgctgag catcacgccc ttcgggtgac cggtggttcc
      421 cgacgagtag agctggaacn nnnnnnnnnn nnnnnnnnnn nnnnnnngcc ggggtcgacc
      481 ggctcctgcg catcgaccca agcgccatag tcgtcgaagc gctcatggcc gccgatgacc
      541 aggatcttgg tgacggtggt gaggttgggc gcgatcgcgt cgaggatcgg gacgaagtcg
      601 ggtccgacca ccaacacctt ggcctgcgca tcgttgacga tgaactcgac ctcgggagcg
      661 gcgagacgcc agttgacatc gacacagacc gcgttcagca ggctcgcgcc gtagaagacc
      721 tcgaagtgct cgatcccgtt cttgtccaag aacnnnnnnn nnnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      961 nnnnnnggcg cagcgttgcg ggggcggcag gcggcgggtc ccgggattgg gggcgggccg
     1021 ctgtcccacc ggtaagttct tggcgtcacc ggtgatgagg gagatcgaca catgacagcg
     1081 acggacgtgc cgggctggaa ctttgccgag atctgggagc atcacgccga tcgcttcccc
     1141 gacgcattgg cccagatcca tggtgaccgc acgttcacgt gggccgagtt cgaccgccgc
     1201 gccgacggca tcgccgccac gctgctcgct cctggcgtac ggcaccagga caaggtggcc
     1261 cactacctct acaactgccc cgagtacctc gagtcgatgt tcggcatgtt caaggccggg
     1321 ctggtcccgg tcaacacgaa ctaccgctac aacgacgacg agatcgtcta cctgtgggac
     1381 aacgccgacg ccgtcgcggc ggtgttccac ggtgccttcg ccgaacgctg cgcagcgctg
     1441 cgcgatcgcc tgcccggcgt gcggacgtgg atctgggtcg acgacggcag cggtccgtgc
     1501 cccgacttcg ccgtgccgta cgagaccgcc gcagccagcg cgaccgaacg gatcgtcgcc
     1561 ccgtggggac gcagcggtga cgacatctac atgctctaca ccggtggcac cacgggcatg
     1621 cccaaagggg tcatgtggcg tcagcgcgac ctgttcggca gcctcgacgc ttcgtcacgt
     1681 acgcagttgc ccgacacgcc cgattgggac gcgctcgacg cgcgggtgtc caaacccggc
     1741 ccacgcaacc tgcccgcggc cccgctgatg cacggcacgg gggcgttcaa cgcgatgtgg
     1801 aatctggcgc tcgcaggcgc gatcgtcacg atggaggggc gccacttcga tccgatcgaa
     1861 ctgctcgaca cggtccagcg catgtcgatc gtcggcgacg cgttcgccaa gccggtgttg
     1921 cgttccctcg atgccgaacc cgatcggtgg gacttcagtt cgatgcgggt catcatctcc
     1981 agcggcgtga tgtggagcgc ggccaccaag tcgggactgc tcgcccacaa cgaccggctg
     2041 atcatggtcg acagcctggg cagcagcgag gcactgggca tggccaacaa caccaccagc
     2101 gcacaaggcg ccggggccaa cgccaccttc gtgctcggcc ccaacgccaa ggtgctcacc
     2161 gacgacgggc gcgaggtggt gcccggcagt ggcgagcggg gccgggtcgc gctgcgcggc
     2221 tacatgccgc tcgggtacta caaggacccg gtcaagtcgg cttcgacgtt catcgagttc
     2281 gaaggcaccc gctggagcat ccccggtgac ttcgccgaaa tcgacgccga cggcaccgtg
     2341 cgcctgctcg gccgcggcag ccagtgcatc aacaccggcg gcgagaaggt gtacccggaa
     2401 gaggtcgaag aggtgctcaa gatgcatccc tcggtgcacg acgcggccgt ggtcggcctg
     2461 gccgacgagc gcttcggtga gaccgtcaac gcgctggtcg aacccaaccc cggcgccgcc
     2521 atcgacgagg ccacgctgat cagcttcgtc aaggagcacc tcgccgccta caaagccccc
     2581 aagcgagtct tcgacgtcac ctccgtcaac cgcgccccca acggcaagct cgactannnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngcgaccgt
     2761 cggattagaa gtccggtgct ctatccagct gagctacgga ggcgcgggcc acattgtggc
     2821 agcgaactag ccctgttggg ctccgaggcg gcgacggagg aagtcgagcg cggtgatgac
     2881 gctcatctgg cgcatctggt cgcgggtacc gggcaggcgc accgtggccg tgaacacctc
     2941 gtcgccgacg ctggcaccga tgcacagcgt gccaacggcg cggccgtcct gttcggctgg
     3001 gccagcgact ccggtgaggg caagaccgac cgtgctcccg accacggcct tgacaccgac
     3061 cgccatctgg gccgcggttt gctcgttgac caccggccct ggctcgacgc cgagcacgga
     3121 gaacttgacc tggctgtcgt aggcgacgac gccgccgcgg aacacctcgc tggaaccggc
     3181 gacgttggtg atccgcccgg cgaccagtcc cccggtgacc gactcggcca cccccaaggt
     3241 gtggccgcgc tcgcgcagga gttgcagcac gaccgactcc atcnnnnnnn nnngtgtcgt
     3301 cgttgaagcc gaacacgaac tcgccgacca ccgcggtgac ccgccgggtc cagcggtcga
     3361 gcaactcgag tgcttcctct tcggtgcccg ccttggcggt gaggcgaatc ttcaaacctt
     3421 cccacccgct ggccagaaac gccagcgtcg ggttggcgat gtcgtcgagt tcggcgatga
     3481 ccgcgtcgag ccgctcgttg agaccgctct cgctctcgcc ccacgtgcgc agcactcggc
     3541 tgacgatgac gtcgtcggaa ccactgcggt cgtggaggtc ggccagcacg gtctgggtga
     3601 acatctcgta catctcatgc ggcacgcccg gcagcgcgta caacaccttg gcccgtccgt
     3661 cgaccatgat cgggcagatc aagcccggcg cggtgccacg ggcgttggnn nnnnnnnnat
     3721 gacggtcgcg ccctgcggca ccaacgcctg gcgcaggttg ttggctgcca tcgtgcggtt
     3781 gcgggccgcg aacagcgagc ggatgtgctc gacgatgtcg tcgtggctga ccagatcggc
     3841 acccatgatc gcggccagcg cttcgcgggt gagatcgtcg tgggtcggcc ccagcccacc
     3901 gcacaccacg accgcgtcgg caccgaccaa ggtcaagcgg agggcatcga cgatgcggct
     3961 caggttgtcg ccgaccttca cctgcgacaa ggagtcgaac ccgtgggcag cgaggtgctc
     4021 accgatccac gccgagttcg tgtcgacgat ctgccccaac aacagctcgg tcccgaccgc
     4081 caccacgtca acccgcatcg gagtcccctt tcgctgcgct acgcgctggc cggatgatcg
     4141 accgggcgat catcgagccc ggcctccttg gcgcggtggg cgcgccacat gtactggatg
     4201 aagctgtaca ccgtcagcac caccgacgtc cacagcaaca ccttccacgt ccacatcgcg
     4261 tcgacagcgg tcagcggcgc gatggcgaac gcgacggcga actgctgggt ggtggtcttg
     4321 atcttggcca gcttgctggc cggcacgttg acacccttgg tgccccagaa gacccggtac
     4381 aggctcatcg ccaactcgcg ggcgaagatg atggccaccg gcacccacca gaactggccg
     4441 ctgtcgatca gcgtgatcat cgccagcagc acgagcagct tgtcggccag gggatcgacg
     4501 aaggtgccga agttggtcgg cccgtgacga cgggccagga acccgtcgat gccgtcgctg
     4561 aagcacagca ggaaccacac caacaacgcc agccagcttc ccttgccgtc gtcgggaaag
     4621 gcgatgaaga cgacgggcga gatgaacatc cgcgccaccg tgacgaggtt ggcccatgtg
     4681 gcgatgcggg tggggtcgac cgtcatctgc cacgctccgt cgcgtcgtcc atctgttcgg
     4741 ccgtctgtcc ggccgcaccg gctgcgacga gatcaggtcc gaaggcattc accaccaaca
     4801 cgtcgtggaa cgtacccgcg gcgaggccgt ccgcgacgtg aaccacgccg tcgatctccg
     4861 gcgcctcgcg gtgggtgcgc ccgacgcctt gctcgtcgac cagcacgcgc acggtgcggc
     4921 cgatgaggct gtcgcggcgc tcggcggtga tcgagtcctg catctcgcgc agctcggcca
     4981 gccgttcgtg catcaagcct tcatcgacgg ccccgtcgag gcccatcgcg taggtaccct
     5041 cctcggggct gtacgcgaag aacccgcacc agtcgagctg ggctgcctcg acgaagtcga
     5101 gcaggcggtc gtggtcttct tcggtctcgc cgggatagcc gacgatgaag ttgctgcgga
     5161 acgcggcctc tggctcacgt ttgcggatgt cgtcgatgcg ggccaggaag cgatcaccgt
     5221 cgccccatcg acgcatccgg cgcagcagcg gcttgctgac gtgctgcaac gacaggtcga
     5281 agtagggcac gcctgtgtca cagatggcat cgaccaacgc gtcggtgagg tcgctgggat
     5341 acaggtacag cagccgggtg cgatcgaccc gagccgcgac cgcctcgatc agcgccacga
     5401 tggagccggc accgagttcg tcggggcggt ctttgccgta gctggccagg tcttgggcga
     5461 tcagcacgat ctcgcgcacg tcgagttgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5581 nnnnnnnnnn nnnnnnntcg gccaccttga cgtaggccca cggcacggcc gacttgggac
     5641 ggggcaggtt gagcagatcg agcgtcggca ccggcgccga actgaccggg atcagcttgc
     5701 gcgcgggcgc gagcacgttg acggacacgc cgaagccagc gacctgatcg acctcgggca
     5761 acgcctcggc caactcggcg ccgtagcgct cggccatgca gccggtgacg accagccgcg
     5821 acccgctggc gcgctggtcg tcgagggcga ggatcgtgtc gatcgactgg tgcgggcgcg
     5881 ccgcatggcc gcccttgccc tcgacatgga tgcggaaatt gtccaccgcc gccnnnnnnn
     5941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6061 nnnnnncccc gccgcaggtc gcaacccggc cgggttcacg ggccgtggtt cagtagttga
     6121 gcgcgagacc gcggtagggc cagcgcagct tgaccagccg cccgctgccc gacagggtga
     6181 tgtacgcgtc gcggaactca ccgctgccgt cgtcgccgaa gcagatgttg gtggtgagcg
     6241 ggtcatcggt cggcacgtgc tcgatccgct gcccgtcggg cgagatgatg gtgaccccgc
     6301 cgttgaccaa cgtggcgaca cacacgttgc cctcaccgtc gaccgcgagc gagtcgagca
     6361 actgcaacc
//
LOCUS       DAORNF010000087         6362 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_9051_length_6362_cov_1.735532, whole genome shotgun sequence.
ACCESSION   DAORNF010000087 DAORNF010000000
VERSION     DAORNF010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 6362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6362
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9051_length_6362_cov_1.735532"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="PLV68_04910"
     CDS             <1..326
                     /locus_tag="PLV68_04910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRE00615.1"
                     /translation="EDDFAYFVDEHASAGFSGGLNWYRSVDHTWATNAPWADRPVTVP
                     SLFIAGEHDCVLQMRGEGALDVMRANVADLRGVHIVPGEGHTVQLEAPDAVNRCLLDF
                     LATLM"
     assembly_gap    386..396
                     /estimated_length=11
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            485..1684
                     /locus_tag="PLV68_04915"
     CDS             485..1684
                     /locus_tag="PLV68_04915"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRE00616.1"
                     /translation="MTASTERLDSRDGAAAIDVRHLSKSFVRRHGERIVPVDDVSFTV
                     AAGEFVVLLGPSGCGKTTLLRCIAGLEQPDQGEIRLGATTVYSSSAKVALPPERRSAS
                     VVFQSYALWPHLTVTKNVAYPLRHGGIPRDEVRARVRDVMAMVGVAHLADQHPGQLSG
                     GQQQRVALARALTAGYDVVLFDEPLSNVDAKVREQLRVDLLAMQRDFGFAALYVTHDQ
                     TEAMELGHRIAVLRDGRIEQLATPVDVYRRPANPYVASFVGRANGFDGEVIAVDGPDV
                     VVSSEIGRMHGTSAEGACAVGDHVHGIIRPESVQIIAPPPSQARDNEWTGQIDDIVFS
                     GPHLNLKVTVGSRSIEVAASSASAIPSLGSQVGLRVEPANFWVLPMEADLNGNPPRPQ
                     PAVSVSP"
     gene            1684..3369
                     /locus_tag="PLV68_04920"
     CDS             1684..3369
                     /locus_tag="PLV68_04920"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HRE00617.1"
                     /translation="MRTRPFDQVTRVVSVGVCLALLFPVGNMILRTFVRGDDRLAVGD
                     VTGLFGDSGTLEAIRNTVIILLIAGPCAIGVGGLLAWLNERTDARIGLAGDVLPIASL
                     LVPPIAGSIAWTLLFSPRSGFANVLIRDGLSRIGINLGQGPVNVFSWYGVIFAYCAYM
                     IPYAYLPIAAALRSLDPALEEAGRVNGSSSLQVLWRITVPAIKPAILTATFLVTVICV
                     GIYSVPSILGTRSRIDVVSVVIVRKLKFSYPPDVKGALLLGFLLLAVVAVVWLLQSRA
                     AGSGRFARIAPRGQSDNLVRLGRWRIFARIGLVGYLVAMSVVPLAALAFVSLQRFWTP
                     HVDWSSLNLDNYRALFGSTGGAARALRNSLWLGLVGATIGVLLAVFAAFYLRRDRSRF
                     SRVVDGVLKLPGNMSHIVLAIAFVLAFAGPPFRLGNTRWILLLAFVSIYVYQAVLQSS
                     DALSRVGDDLIDASHTSGHGITRTVRWVVLPLMAPGLVASWILFFVHIIGDVNAAAIL
                     AGTKNQVVGSELVNLYEGGQYTQLAALATVLSAVSMVFVLSSLGLSRLMRRRT"
     gene            3458..>3742
                     /locus_tag="PLV68_04925"
     CDS             3458..>3742
                     /locus_tag="PLV68_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00618.1"
                     /translation="MAFGLVVAACGSDDNNSKATTTSAAAAASTTAADSEVTTSAALV
                     EAAKKEGSVVFYSAYNAELSQQLAAAFKDEYGIDVEVVRQASADLNARFAA"
     assembly_gap    3743..3781
                     /estimated_length=39
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3782..>4181
                     /locus_tag="PLV68_04930"
     CDS             <3782..>4181
                     /locus_tag="PLV68_04930"
                     /inference="COORDINATES: protein motif:HMM:NF024739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRE00619.1"
                     /translation="DVLWQPDDVFAKAATDKGWLAQLDPARIPGLDQVPADDRTDTYA
                     AVLTQPWGIAYNTDLVKGDQIPTTWTDLADGPAIDGGLLVANPANSVSTSAVYNFWLD
                     QFGEEFFTKLRDTQRFNIGDSVSNAIQQVGA"
     assembly_gap    4182..4253
                     /estimated_length=72
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4254..4509
                     /locus_tag="PLV68_04935"
     CDS             <4254..4509
                     /locus_tag="PLV68_04935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRE00620.1"
                     /translation="VIPDDTTGFSLLAAISNSAPHPSAAELFVSFLLSEQGQAIAVAD
                     IAIPVLPSVSAAVQRPAGYVPSDNAATAARMDQLTSLLGA"
     gene            4519..5496
                     /locus_tag="PLV68_04940"
     CDS             4519..5496
                     /locus_tag="PLV68_04940"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HRE00621.1"
                     /translation="MQFGIKLPNLSPLASRRSILDGRAAEDNGYASVWASDHLVFLPT
                     TVHDAANRRFPVAFDTPAVDPLIALSLVAGVTERVSLGTSVLVLANRNPVAIAKAWAS
                     LDHLAPGRVLAGVGTGWQRPEFKALGVDDRFPWRGSVTEEFIDVLRRCWTDDLPSFHG
                     RFYEFEPLHFFPRPAAPIPILLGATSDRGVARVGRVADGFHGTNLTPPAAAEMIRRIR
                     EAASDAGRDPAGLRFTTLCELDVQPEGGVPEDSDIHLVGSPQEIVDRVGRFAAAGIEH
                     IALRVRALSGSTRRGIEPTLSLEVGLDQLHAFAELAAAEFDAHVPSPEG"
     assembly_gap    5746..5899
                     /estimated_length=154
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5900..6130
                     /locus_tag="PLV68_04945"
     CDS             <5900..6130
                     /locus_tag="PLV68_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family DNA-binding protein"
                     /protein_id="HRE00622.1"
                     /translation="EAADLLGVSRPTLVKLLEIGEIPFEQPGRHRRVRLADVLAYRER
                     ASGERRAALDRMVEIADEADMYERTATPKRTR"
     gene            6152..>6362
                     /locus_tag="PLV68_04950"
     CDS             6152..>6362
                     /locus_tag="PLV68_04950"
                     /inference="COORDINATES: protein motif:HMM:NF024861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HRE00623.1"
                     /translation="MLDTCVLYPAHLRDTLLRLAERGMYRVLWSADIVDELHRNLVED
                     GLTARSVDRLIAEMAVAFPDAEVAGY"
BASE COUNT          890 a         2168 c         1968 g         1060 t
ORIGIN      
        1 cggaggacga tttcgcctac ttcgtcgacg agcacgcctc cgccgggttc agcggtggcc
       61 tcaactggta ccgcagcgtc gaccacacgt gggcgacgaa cgcgccctgg gccgaccggc
      121 cggtgaccgt gccgtcgctg ttcatcgctg gtgagcacga ctgcgtgctg cagatgcgcg
      181 gcgaaggcgc cctcgacgtg atgcgggcga acgtcgctga cctgcgcggg gtgcacatcg
      241 tccccggcga gggtcacacg gtgcagctgg aagcgcccga cgccgtcaac cgatgcctgc
      301 tggatttcct cgcgacgctg atgtgatccg cgcggccgag gccgagggcc gaagggccgc
      361 cgtcgtgctc gcctctgggg ccggcnnnnn nnnnnngacc ctttgagttc gatccggacc
      421 acccgtacgg tgctatcagg aggtgcccca tgtcacgttt tcgcaagtac ccccagagcg
      481 tctcttgacc gccagcacgg agcggctcga tagtcgcgac ggtgcagcgg cgatcgacgt
      541 ccgccacctg agcaaatcgt tcgtccgccg tcatggtgag cggatcgtgc ccgtggacga
      601 cgtcagcttc accgtcgccg ccggcgagtt cgtcgtgctg ctcggcccga gtgggtgcgg
      661 taagacgacg ttgctgcgtt gcatcgccgg cctcgagcag cccgaccagg gcgagatcag
      721 gctcggcgcc accaccgtgt actcctcgtc ggccaaggtt gcgctgccgc ccgagcgccg
      781 ctcggcgagc gtcgtgttcc agagctacgc cctgtggccg cacctgaccg tgacgaagaa
      841 cgtcgcctac ccgctgcgcc acggggggat tccacgcgac gaggtgcggg cgcgggtgcg
      901 ggacgtgatg gcgatggttg gtgtcgccca cctcgccgac cagcatccgg gacagctcag
      961 cggcggccag cagcagcgcg tcgcgctggc acgcgccctc accgcgggct acgacgtcgt
     1021 gttgttcgac gagccgctgt ccaacgtcga cgccaaggtc cgcgaacagt tgcgcgtcga
     1081 cctgctggcc atgcagcgcg acttcggctt cgccgcgctc tacgtcaccc acgaccagac
     1141 cgaggcgatg gagcttggcc accggatcgc cgtgctgcgc gacggcagga tcgagcaact
     1201 cgccacgccg gtcgatgtct accggcgccc ggccaacccg tacgtcgcca gctttgtcgg
     1261 tcgtgccaac gggttcgacg gcgaggtcat cgccgtcgac ggtcccgatg tcgtcgtgtc
     1321 gagcgagatc ggcaggatgc acgggacgtc cgctgagggt gcgtgcgccg tcggtgacca
     1381 cgtgcacggg atcatccggc cggagtcggt gcagatcatt gccccaccgc cgagccaagc
     1441 gcgcgacaac gagtggacgg gccagatcga cgacatcgtg ttcagcgggc cgcacctgaa
     1501 cctcaaggtc accgtgggta gccgttcgat cgaggtcgcg gccagcagcg cgtcggccat
     1561 cccttccctc gggagccagg tggggctgcg cgtcgagccc gccaacttct gggtcctgcc
     1621 gatggaagcg gacctcaacg gcaacccgcc acgcccacaa cccgccgtgt ccgtgagccc
     1681 gtgatgagga ccaggccgtt cgatcaggtg acgcgggtcg tttccgtggg cgtctgcctc
     1741 gccctgctgt tccccgtcgg caacatgatc ctgcggacct tcgtgcgcgg tgacgaccgc
     1801 ctggccgtcg gcgacgtcac cggtctgttc ggcgacagcg ggacactcga ggccatccgc
     1861 aacacggtga tcatcctgct catcgccggg ccgtgtgcga tcggcgtcgg cgggttgctc
     1921 gcctggctca acgagcgcac cgacgcgcgc atcggcctcg ccggtgacgt gctgccgatc
     1981 gcctcgctgc tcgtgccgcc gatcgccggc tcgatcgcct ggaccctgct gttttcgcca
     2041 cgcagcggct tcgccaacgt cctcatccgt gacgggttgt cgcgcatcgg catcaacctc
     2101 ggccagggcc cggtcaacgt cttttcgtgg tacggggtga tcttcgccta ctgcgcgtac
     2161 atgatcccgt acgcctactt gccgatcgcc gccgcgctcc gctctctcga cccggccctg
     2221 gaagaggccg gtcgggtcaa cggatcgtcc agcctgcagg tcttgtggcg catcaccgtc
     2281 cccgcgatca agccggcgat cctgaccgcg acgttcctcg tcaccgtcat ctgcgtcggc
     2341 atctactccg tgccgagcat cctcgggacg cgctcgcgca tcgacgtcgt ctcggtcgtc
     2401 atcgtccgca agctgaagtt ctcgtacccg ccggacgtga agggcgcgct gctgctcggc
     2461 ttcctgctcc tcgcggtcgt cgccgtcgtt tggctcctcc agtcgcgggc ggcgggttcc
     2521 ggccggttcg cgcgcatcgc cccccgtggc cagtccgaca accttgtccg gctcggtcgg
     2581 tggcggattt tcgcccgcat cggcctggtc ggctacctcg tcgcgatgtc agtcgtgccg
     2641 ctcgccgcgc tggcgttcgt ctcactgcaa cgcttctgga cccctcacgt cgactggtcg
     2701 tcgctgaacc tggacaacta ccgggcgctg ttcggctcga ccggaggggc agcccgcgcg
     2761 ttgcgcaaca gcctgtggct cggcctcgtc ggcgccacca tcggcgtact cctcgcggtc
     2821 ttcgccgcgt tctacctgcg ccgtgaccgc tctcggttct cgcgggtcgt cgacggcgta
     2881 ctcaagctgc ccgggaacat gtcgcacatc gtgttggcga tcgctttcgt gctcgcgttc
     2941 gccggaccgc cgttccgcct cggtaacacg cgctggatcc tgctgctcgc gttcgtctcg
     3001 atctacgtgt accaggccgt cctgcaatcg tccgacgcgc tgtcccgcgt cggcgacgac
     3061 ctcatcgacg cctcgcacac ctcgggccac gggatcacgc gcaccgtgcg ctgggtggtg
     3121 ttgccgctga tggcccccgg gctcgttgcc tcgtggatcc tgttcttcgt ccacatcatc
     3181 ggcgacgtca acgctgcagc gatcctcgcc ggcacgaaga atcaggtcgt cggctccgaa
     3241 ctggtcaacc tctacgaagg cggccaatac acccagctcg ccgcgctggc gacggttctc
     3301 agcgcggtgt cgatggtgtt cgtcctctcg tcactcggcc tgagccgact catgcgacga
     3361 cgcacctgac ccgagccatc gaaccgaaac caccaatacc cgaggagacc aacccatgag
     3421 aacccgccga tccctgacgc gcctgctggc agtgcccatg gcattcggac ttgtcgttgc
     3481 tgcctgcggg agcgacgaca acaactccaa ggcgacgacc acctccgccg cagctgccgc
     3541 gtcgacgacc gctgccgact cggaggtcac gacctccgcc gcgctcgtcg aagcggcgaa
     3601 gaaggaaggt tccgtcgtgt tctattcggc atacaacgcc gaactgagcc agcagctggc
     3661 cgctgcgttc aaggatgagt acggcatcga cgtcgaggtc gtgcgccagg caagcgccga
     3721 cctgaacgcc cgcttcgccg ccnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 ncgacgtcct ctggcagccc gacgacgtgt tcgccaaggc cgcgacggac aagggctggc
     3841 tcgctcagct cgaccctgcg cggatccccg gtctggacca ggtgccggcc gacgaccgga
     3901 ccgacaccta cgctgccgtg ctcacccagc cgtggggcat cgcctacaac accgacctcg
     3961 tcaagggcga ccagatcccg accacctgga ccgatctggc cgatggcccg gcgatcgacg
     4021 gtggcctact cgtcgccaac ccggcgaaca gcgtctcgac gtcggccgtg tacaacttct
     4081 ggctcgacca gttcggtgag gagttcttca ccaagctacg cgacacccaa cgctttaaca
     4141 tcggcgactc ggtcagcaac gccatccagc aggtcggcgc gnnnnnnnnn nnnnnnnnnn
     4201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncgtcatc
     4261 ccggacgaca cgaccgggtt ctccctgctg gccgcgatct ccaactcggc cccacacccg
     4321 agcgcggcgg aactgttcgt gtcgttcctg ctgtccgagc agggacaggc catcgccgtc
     4381 gccgacatcg ccatcccggt gctgccctcg gtcagcgccg ctgtgcagcg gccggctggc
     4441 tacgtgccgt ccgacaacgc tgcgacggcc gcacgcatgg accagttgac gtcgcttctc
     4501 ggagcctgaa ccgacgcgat gcagttcgga atcaagctgc ccaacctcag cccgctcgcc
     4561 tcacgacgat caatcctcga cgggcgagcg gccgaggaca acggatacgc gtccgtctgg
     4621 gccagtgatc acctcgtgtt tctgccgacg acggtgcacg acgcggcgaa ccgacgcttt
     4681 cccgtcgcgt tcgacacgcc ggccgtcgac ccgctgattg cattgtcgct ggtggccggc
     4741 gtgaccgagc gagtttcgct cggcacgtcg gttctggtgc tggcgaaccg caatcctgtc
     4801 gccatcgcca aggcgtgggc ctcgctcgac cacctcgcgc ccggtcgggt cctcgccggg
     4861 gtcggcaccg gctggcagcg gccggagttc aaggcgctgg gcgtcgacga ccgcttcccc
     4921 tggcgcggct cggtgaccga ggagttcatc gacgtcctcc ggcgctgctg gaccgacgac
     4981 cttccgagct tccatggtcg cttctacgag ttcgagccgc tgcacttctt ccccagaccg
     5041 gcggcgccga tcccgatcct gctcggcgcg acgagcgatc gtggcgtggc ccgggtcggg
     5101 agggtcgccg acggcttcca cggcacgaac ctcacccctc cggccgccgc cgagatgatc
     5161 cggcggattc gcgaggcagc gagcgacgcc ggccgggatc cggcaggact gcggtttacg
     5221 acgctgtgcg agctcgacgt ccagcccgaa ggtggcgtgc ccgaggactc ggacatccac
     5281 ctcgtcggat cgccgcagga gatcgtcgac cgcgtcggac ggttcgcagc cgccgggatc
     5341 gagcacatcg cgctgcgtgt gcgtgccctg tcgggctcga cccgacgagg catcgaaccg
     5401 acgctctcgc tggaagtcgg cctcgaccaa ctgcacgcct tcgccgagct tgccgcggct
     5461 gagttcgatg cgcacgtgcc ttcaccggag ggctgaggcc gatcgcccgt ccacgcgggc
     5521 ctgcgttgag gtcggcggtg agcactgcga tcgattggcg tggagcgttt catcatctcg
     5581 ttgctgagtt cggccgaagt ggaggcgctt gcgaattttt cgatttgcag tacgatgacg
     5641 gcctgaggag gagcacccac atgacgacgt ccacgctcga acgcacggtc ctaccgccag
     5701 tcgagtcgct tgacgcgctc gtcgcaatgc tcagccggcc tggtgnnnnn nnnnnnnnnn
     5761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5881 nnnnnnnnnn nnnnnnnnng aggccgccga cttgctcggc gtcagccggc ccacgttggt
     5941 gaagcttctc gaaatagggg agatcccgtt cgagcagccg gggcgtcatc gcagggtgcg
     6001 ccttgccgac gtgctggcgt atcgcgaacg tgcctcaggt gaacgccgtg ctgcgctcga
     6061 tcgcatggtc gagattgccg atgaggctga catgtacgaa cggaccgcga cgcccaagcg
     6121 cacccgctga ggatccgtgc ccttcgcagt cgtgcttgat acgtgtgtgc tctacccggc
     6181 acacctccgt gacaccctgc tccgcctggc ggagcgtggg atgtatcgcg tgctgtggtc
     6241 cgccgacatc gtcgacgagc tgcaccgcaa cctcgtcgag gatggcctca ccgccaggtc
     6301 cgtcgaccgg ctcatcgccg agatggccgt tgcgttcccg gacgcggagg tggctggcta
     6361 tc
//
LOCUS       DAORNF010000088         6355 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_9075_length_6355_cov_1.886984, whole genome shotgun sequence.
ACCESSION   DAORNF010000088 DAORNF010000000
VERSION     DAORNF010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 6355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6355
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9075_length_6355_cov_1.886984"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            139..>852
                     /locus_tag="PLV68_04955"
     CDS             139..>852
                     /locus_tag="PLV68_04955"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRE00624.1"
                     /translation="MSAHAFGDRRSIAVVGAGVAGLTAAYVLAQQHEVTLYEADGRLG
                     GHAHTHDVVDPAGNVLAVDTGFIVHNDRTYPHLRRLFAELGVQTRPTEMSMSIHDERS
                     GVEFAGGRGAKGVFAQPRRALDPRFLSVLVQVKRFQMRANAFLRRTDDSDRTTYGEFL
                     RTHRFSDAFVRLYAVPLVSCVWSMGTGAALDYPARYLFRFLDHHGLLELYGSPQWYTV
                     VGGSRTYVEAIGIRLADVRQ"
     assembly_gap    853..872
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <873..1417
                     /locus_tag="PLV68_04960"
     CDS             <873..1417
                     /locus_tag="PLV68_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00625.1"
                     /translation="HDTGVSLQDSSGAWSDYDGVVIATHADQALDLLADASREEKEVL
                     GAFRYSRSETILHTDERLLPTAPQARASWNYLTPSATDGDRAPIVTYWMNRLQRLDSS
                     EHYLVTLNARDRIDPTKVVAVMSYEHPIYDLPAIEAQARLASLATPRTAFAGAYHGWG
                     FDEDGCRSGVEAARTLGVAW"
     gene            1414..2196
                     /locus_tag="PLV68_04965"
     CDS             1414..2196
                     /locus_tag="PLV68_04965"
                     /inference="COORDINATES: protein motif:HMM:NF018770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1365 domain-containing protein"
                     /protein_id="HRE00626.1"
                     /translation="MTASVQSTHDASVLPVLPAIVDGVVAHRRKGPIRYEFRHRVYLW
                     LVDLDALPRPPWYLRPVARFDARDHLGGTGPGASRRIKANVERFLAQRDVHLGHGARV
                     VMLANARVFGHVFDPLTMFWCFAGDGTLRCVVAEVHNTYGERHVYLLEPGPDGEASTD
                     KQFYVSPFNDVSGYYAMRFTLTQQHVDVTVALHRDGHPVFDASFTGSPTPATTAAIAR
                     FALERPAMTQRVTASIRLHGVRLWWRGLPTVTRPSHVRQEGV"
     gene            2198..2953
                     /locus_tag="PLV68_04970"
     CDS             2198..2953
                     /locus_tag="PLV68_04970"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRE00627.1"
                     /translation="MTDKHRFAGRTAWIVGASSGIGAALAAELAGRGARVAISARRKS
                     ALAAVADGEMAVVPVDVTDRAALAGAADRVRDELGEIDMVVYNAGFWEQMDLSDWDCD
                     VFARHVEINVLGLNNCLGAVLPHMIKAGSGHLVGVASVAGYRGIAGAEAYGATKAAQI
                     NMLEALRTSAAPHGIAVTTVCPGFVRTDLTAKNAFPMPFMIDAQTAARSICNGVERGQ
                     MEIVFPLPMAVAMKIARLLPVRVWAAAMRRVTK"
     assembly_gap    2993..3074
                     /estimated_length=82
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3075..3555
                     /locus_tag="PLV68_04975"
     CDS             <3075..3555
                     /locus_tag="PLV68_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00628.1"
                     /translation="RAVAELPLRVEFPDGTSLGAGRDTDPCMVVNRPGAFFGRLAVDA
                     KIGFGEGYMVGDWDTGPGTDLADLLTPFAGNVATLVPRPLQRFRRLVERRQPADEQGT
                     PSQARRNIHRHYDLSNDLFSPRSSTRRSATRRRCSQTGMISPPPNVARSTPSWTSRR"
     gene            3480..4211
                     /locus_tag="PLV68_04980"
     CDS             3480..4211
                     /locus_tag="PLV68_04980"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HRE00629.1"
                     /translation="MFADGDDLATAQRRKIDAILDLASVGPGCEMLEIGTGWGELAIR
                     AAQRGAHVTTVTLSTEQRDLALRRVAAAGVSGRVRILVADYREVAGQYDAIVSVEMVE
                     AVGRRFWRDYFSTLDRLLRPGGRIGLQSITVSHDRLLATQHSYTWIHKYIFPGGIVPS
                     IEAIDTTLQTHTSLAIAERRDFGPDYATTLKCWRDTFLANWPTIESDRFDGVFKRMWE
                     FYLAYCEAGFRVSYLGVSQFALVRS"
     gene            complement(4244..>4501)
                     /locus_tag="PLV68_04985"
     CDS             complement(4244..>4501)
                     /locus_tag="PLV68_04985"
                     /inference="COORDINATES: protein motif:HMM:NF021604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HRE00630.1"
                     /translation="DRNGLLLLQLSTSGLDPGDGFLEVWVIDPEVTQLVSLGPLRNDG
                     IYELPPGLDPAAFPIVDVSVEPIDGVPTHSGASVLRGQLTF"
     assembly_gap    4502..4591
                     /estimated_length=90
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4840..4874
                     /estimated_length=35
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4913..5479)
                     /locus_tag="PLV68_04990"
     CDS             complement(4913..5479)
                     /locus_tag="PLV68_04990"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRE00631.1"
                     /translation="MRAEPASREAADDAVAGRWVAGDDVALRLAYEQFGTLIFTFCSR
                     ALVDRGAAADCTQETFLSAWRSRDGYDPKRGSLAGWLMGIARFKVLDAYRAAARIPAP
                     RDDLAGDDVTEADSYDDQLADRLLIAHALEALSPRARRVVEMAFYSDLTQTEIAETMG
                     LPLGTVKSDLRRGLLRLRSHLEGSGGNA"
     assembly_gap    5832..5849
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5850..6329
                     /locus_tag="PLV68_04995"
     CDS             <5850..6329
                     /locus_tag="PLV68_04995"
                     /inference="COORDINATES: protein motif:HMM:NF014522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HRE00632.1"
                     /translation="VAPATTVAETTSDTTGEADIVDTAIAAGDFTTLVAAVQAAGLEE
                     TLRGEGPFTVFAPTDDAFGALPAGTVDTLLEDPTGDLAAILTYHVVAGEVLAADVVGL
                     DGQEVATVNGATFTVNVGDDGSVSLTDAAGNQVGVVTTDIRTSNGVIHVIDAVLMPG"
BASE COUNT          973 a         2193 c         1989 g          955 t
ORIGIN      
        1 gcgggtggcc tgcgacgcgg gtgacgccat cgggttcggc atcgccgcgc caccgtcgtc
       61 gcgggcgaag gtgatcgcag tcgccgcggg ctggggcgcg atctgcgcct ccgcctgccc
      121 tgccaccggt cggcgaccgt gagcgcccac gccttcggcg accgtcgctc gatcgcggtc
      181 gtcggagccg gcgtcgccgg tctgaccgcc gcgtacgtcc tggcgcagca gcacgaagtg
      241 accctctacg aagccgacgg tcgtctcggt ggccacgccc acacccacga cgtcgtcgat
      301 cccgccggca acgtgctcgc ggtcgacacc ggtttcatcg tccacaacga ccgcacctat
      361 ccgcacctgc gtcgactgtt cgccgaactg ggggttcaga cacgcccgac cgagatgagc
      421 atgagcatcc acgacgagcg cagcggtgtc gagttcgcgg gcgggcgcgg cgccaagggc
      481 gtgttcgccc agccccgccg tgcactcgat ccccgcttcc tctcggtgct ggtccaggtc
      541 aagcggttcc agatgcgggc taacgccttc ctacgcagga ccgacgacag cgaccggacc
      601 acgtacggcg agttccttcg cacgcaccgc ttctccgacg ccttcgttcg gctgtacgcg
      661 gtgccgctcg tgtcgtgcgt gtggtcgatg ggcaccggtg ccgcgctgga ctacccggcc
      721 cggtacctgt tccgattcct cgaccatcac ggtctgctcg agttgtacgg ctcgccgcag
      781 tggtacaccg tggtcggcgg atcacgcacg tacgtcgagg cgatcggtat ccgactggcc
      841 gatgtgcgcc agnnnnnnnn nnnnnnnnnn nngtcacgac accggggtct cgctgcagga
      901 ctcctcgggc gcatggagcg actacgacgg ggtggtcatc gcgacccacg ccgaccaagc
      961 gctcgacctg ctggccgatg ccagccgcga ggagaaagag gtcctcggag cgtttcgtta
     1021 ctcccgcagc gagaccatcc tgcacaccga tgagcggttg ctcccgaccg caccgcaggc
     1081 gcgggcgtcg tggaactacc tcacgccgtc ggcgaccgac ggcgatcggg ccccgatcgt
     1141 cacctattgg atgaaccgcc tccaacgcct cgacagctct gagcactacc tggtgacgct
     1201 caacgcccgc gaccggatcg accccaccaa ggtggtcgcg gtgatgagct acgagcaccc
     1261 gatctacgac ctgccggcga tcgaggcgca ggcccggctg gcctcgctgg cgacgcctcg
     1321 caccgcgttc gcgggtgcct accacggctg gggattcgac gaggacggct gccgctcggg
     1381 cgtcgaggcg gcgcgtactc tcggagtggc ctggtgaccg cgtccgtcca gtcgacccac
     1441 gacgcgtcgg tgctgccggt gctgcccgcg atcgtcgacg gtgtcgtcgc tcatcgccgc
     1501 aaaggtccga tccgctatga gttccgccac cgcgtctacc tatggctcgt cgacctcgac
     1561 gcactccccc gcccaccgtg gtatctgcgg ccggtcgccc ggttcgacgc ccgcgaccac
     1621 ctcggaggca cgggtcccgg ggcgtcgcgc cgcatcaagg cgaacgtcga acggttcctg
     1681 gcccagcgcg acgtgcacct cggccacggc gcgcgggtcg tcatgctcgc caacgcgagg
     1741 gtgttcgggc acgtgttcga cccgctgacg atgttctggt gcttcgccgg tgacggtacg
     1801 ttgcgctgcg tcgtcgccga ggtgcacaac acgtacggcg agcggcacgt ctacctactg
     1861 gagcccggtc cggacggaga ggcgagcacc gacaagcagt tctacgtgtc accgttcaat
     1921 gacgtgtcgg ggtactacgc gatgcgcttc acactcaccc agcaacacgt cgacgtgacg
     1981 gtcgccttgc accgagacgg gcaccccgtg ttcgacgcca gcttcaccgg ctcacccaca
     2041 cctgcaacga cggcggcgat cgcgcggttc gcactcgagc ggccagcgat gacacagcgg
     2101 gtcacagcgt cgatccgtct ccacggcgtc cggctgtggt ggcgcggcct accaactgtg
     2161 acccgcccgt cccacgtgag acaggaagga gtctgagatg accgacaaac atcgcttcgc
     2221 cgggcgcacg gcctggatcg tcggggcatc gagcgggatc ggcgccgcgc tcgccgccga
     2281 gctcgcagga cgaggtgcca gagtggcgat ctctgcccga cgaaagagtg cgctggcggc
     2341 cgttgccgat ggcgagatgg ccgtcgttcc ggtcgacgta accgaccgcg cggccctcgc
     2401 cggcgccgcc gaccgtgttc gtgacgagct cggcgagatc gacatggtcg tctacaacgc
     2461 cggcttctgg gaacagatgg acctgtccga ctgggactgt gacgtgttcg cccgccacgt
     2521 cgagatcaac gtgctgggcc tcaacaactg cctcggcgcg gtactgccgc acatgatcaa
     2581 ggccgggagc ggccacctcg tcggcgtggc gtcggttgcc ggatatcgcg gcatcgccgg
     2641 agccgaggcc tatggcgcga ccaaggcagc gcagatcaac atgctcgaag cgctgcggac
     2701 gtcggcggcg ccacacggca tcgccgtcac gacggtgtgc cccggattcg tccgcaccga
     2761 cctcaccgcc aagaacgcct tcccgatgcc gttcatgatc gacgcccaga cagcggcccg
     2821 gtcgatctgc aacggcgtcg aacgcggcca gatggagatc gtgttcccgc tgccgatggc
     2881 cgtcgcgatg aagatcgccc gcctgctccc cgtccgggtg tgggccgcgg cgatgcgccg
     2941 cgtgacgaaa tgaacgccgc cgcgacgaga accggcgctg cgcgcgttga cgnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnccgagc cgtcgccgaa ctgccgttgc gcgtcgagtt ccccgacggc
     3121 acctcgctcg gcgccgggcg cgacaccgac ccatgcatgg tggtcaaccg accgggcgcg
     3181 ttcttcggcc gtctcgccgt cgacgccaag atcggcttcg gtgagggtta catggtcggc
     3241 gactgggaca ccggccccgg caccgacctc gccgacttgc tcaccccatt cgccggcaac
     3301 gtggcgacgt tggtgccccg gccgctgcag cggttccgac ggctcgtcga gcgccggcaa
     3361 ccggctgacg agcagggcac gccgtcgcag gcgcgccgga acatccaccg ccactacgac
     3421 ttgtccaacg acttgttttc gcctcgttcc tcgacgagac gctcagctac tcgtcggcga
     3481 tgttcgcaga cggggatgat ctcgccaccg cccaacgtcg caagatcgac gccatcttgg
     3541 acctcgcgtc ggtagggccg ggttgcgaga tgctcgagat cggcacggga tggggcgagc
     3601 tcgccatccg ggcggcccaa cgcggcgccc acgtcaccac ggtcacgttg tcgaccgagc
     3661 agcgcgacct cgcgctccgg cgcgtcgccg ccgctggcgt gtccggccgg gtccgcatcc
     3721 tcgtcgccga ctacagggag gtggccggac agtacgacgc gatcgtcagt gtcgagatgg
     3781 tcgaggcggt cggccgtcgc ttctggcgcg actacttctc gacgctcgac cggctgctcc
     3841 gacccggtgg tcggatcggc ctgcaatcga tcaccgtctc acacgaccgg ctgctcgcca
     3901 cccagcactc gtacacgtgg atccacaagt acatcttccc gggcggcatc gtcccgtcga
     3961 tcgaggccat cgacaccacg ctgcagaccc acacgtcgtt ggcgatcgcc gaacgccgcg
     4021 atttcggtcc cgactacgcg acgacgttga agtgttggcg tgacacgttc ctcgccaact
     4081 ggccgacgat cgagagcgac cggttcgacg gcgtcttcaa gcggatgtgg gagttctacc
     4141 tcgcctactg cgaagccggg tttcgcgtca gctacctcgg cgtgagccag ttcgccctcg
     4201 tcagatcatg aatcagatcg tgggtcagat cgtgacgtgg cggtcagaac gtgagctggc
     4261 cgcgcagcac gctggcgccg gagtgggtcg gaacgccgtc gatcggttcc accgacacgt
     4321 cgacgatggg gaacgccgcc gggtcgagtc cggggggaag ctcgtaaatg ccgtcgttgc
     4381 gcaacggtcc gagtgagacg agttgggtca cctccgggtc gatcacccac acctccaaga
     4441 agccatcgcc tgggtcgagg ccactcgtgg agagctggag cagcagcaac ccgttccgat
     4501 cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nccggccgcg atcacggcca cgacggcggc
     4621 ggccgcagca acgccccaga tccgccgatc gcggcgtcgt tgcgcgcggc cttgaccagt
     4681 ggtcgacctc cgccggtcgt cgatggcacg aacgtttccg gactcgcgcg aaacgtccgc
     4741 agctggctcg tcaccgggcc cggtcgtgac gtccgctgcg gcgatgcggt cccacagatc
     4801 ggacggtggg gactcccatg cgactgtcac gggatcgacn nnnnnnnnnn nnnnnnnnnn
     4861 nnnnnnnnnn nnnncggcgt cgccttcggt cgcgtcgggg tcttgcgtca cgtcaggcat
     4921 tccctccact tccttccaga tgcgagcgaa gccggagcag accacggcgg aggtcgctct
     4981 tcaccgtacc gagtgggagt cccatcgtct ctgcgatctc ggtctgtgtc agatccgagt
     5041 agaacgccat ctcgacaacg cggcgggccc gaggcgagag cgcctcaagg gcgtgcgcga
     5101 tcagcaaccg atcggcaagc tggtcgtcgt acgagtcggc ttcggtcacg tcgtcgccgg
     5161 caaggtcgtc gcggggcgcc gggatccgcg ccgcggcccg gtacgcatcg aggactttga
     5221 agcgggcaat acccatcaac cacccagcga ggctgccgcg cttcgggtcg tagccatcgc
     5281 gggaccgcca tgcgctgaga aaggtctcct gtgtgcagtc ggcagccgcc ccgcggtcga
     5341 cgagcgcccg cgagcagaac gtgaagatca gcgtgccgaa ctgctcgtac gcgagccgca
     5401 gcgccacgtc gtcaccagcg acccatcgcc cggcgaccgc gtcgtcggcc gcttcccgac
     5461 tcgcaggctc agcgcgcacc gcgccacggt atcggatgtt cgaccagcat cggggttggc
     5521 aactacgggc cgggtcgggg ctggcgagga gggcatgcat cagagatgtc cctgtgtcgg
     5581 tgatcggaca cccacccctt cgtgccggac gttctcggcg gatgcgggag cacgacagac
     5641 gaaatcctgc cgaagtgcat ccgtatagca cccgattccc gaaggggacc tcgaaagtcc
     5701 tgtccccttc ccaaggagct ccccgatgaa cacccgattc gttcgactcg ctgctggcct
     5761 cgccgccgtc agcttgctcg ccgccgcatg cggcgacgac gagtcgagcg acaccgcgac
     5821 caccgcggcc gnnnnnnnnn nnnnnnnnng tggcgccggc gacgaccgtg gccgagacga
     5881 cttccgacac gacgggggag gccgacatcg tcgacaccgc catcgcagcc ggcgacttca
     5941 ccaccctcgt cgccgccgtc caggcagccg gcctcgaaga gacgctgcgc ggcgaagggc
     6001 cattcacagt gttcgcccca accgacgacg ccttcggggc actccccgcc ggcaccgtcg
     6061 acacactgct cgaagaccca accggcgacc tcgccgcgat cctcacctac cacgtcgtcg
     6121 ccggtgaagt gctcgccgcc gacgtcgtcg gactcgacgg ccaagaagtc gccaccgtca
     6181 acggcgccac cttcaccgtc aacgtcggcg acgacggttc ggtgtcactc accgacgccg
     6241 ccggcaacca ggtcggcgtt gtcaccaccg acatccggac cagcaatggc gtcatccacg
     6301 tcatcgacgc cgtcctcatg cccggctgat ccactcaaag ttgcccccac ccccc
//
LOCUS       DAORNF010000089         6316 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_9146_length_6316_cov_1.694298, whole genome shotgun sequence.
ACCESSION   DAORNF010000089 DAORNF010000000
VERSION     DAORNF010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 6316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6316
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9146_length_6316_cov_1.694298"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..433)
                     /locus_tag="PLV68_05000"
     CDS             complement(<1..433)
                     /locus_tag="PLV68_05000"
                     /inference="COORDINATES: protein motif:HMM:NF022182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HRE00633.1"
                     /translation="MSELHPDDSVGAMAAAIDAWLTGFADTNPAIAAVDRDPATARWY
                     VRMRGDEKEFITVWLTLGQRTLQYEAYVMPAPEENVAAVYENLLRRNQQLIGHHFAIG
                     VEDAVFLRGDLPLGVLCEDELDRVIGSLYATTELCFRSLLRL"
     gene            complement(430..>630)
                     /locus_tag="PLV68_05005"
     CDS             complement(430..>630)
                     /locus_tag="PLV68_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00634.1"
                     /translation="NGYLVEGRDPDRYAARIAAILDDPTLATSMGMSGAERARRYTWG
                     TAAGCLDRVYADLAVHSLAACS"
     assembly_gap    631..801
                     /estimated_length=171
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<802..1631)
                     /locus_tag="PLV68_05010"
     CDS             complement(<802..1631)
                     /locus_tag="PLV68_05010"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRE00635.1"
                     /translation="MQRVAVLSLHTSPLAQPGSGDSGGMNVYVRELVSSLAQRGVECT
                     TYTRASRPDQPRVVDIEPGHRVVHIPAGSFDLAKDDLHHVVDEFGDRVIEDLHDGPGA
                     DVLHANYWLSGLVAHRIKHELDVPFVSTFHTLARVKAEGGDPEPLWRERAEAEIINCA
                     DGICVSCTEEERQFRRLYGDPPGRIEIVAPGVEHALFAPGHQGGARRALGMGPGPVLL
                     FVGRIQPLKGVDVAVRALAALGRPDARLVVVGGASGLEGGSEVQRVHALIDELGVASQ
                     V"
     gene            complement(1700..>2305)
                     /locus_tag="PLV68_05015"
     CDS             complement(1700..>2305)
                     /locus_tag="PLV68_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00636.1"
                     /translation="RWRVRAGTSFGISSAISSGGQSDLFQFAEGDLMSAQTTPTLDPS
                     ELSLADLRAERATLQAADDAVSYVRRLVQARLDMVELERKHRLAGTQFNVDEDLPAVL
                     GRHLTGGPARPPRPTADASDHPLAIELDELCRRLGADRLDELSDEQLDAVATELASFE
                     QERSHERQVDKRFGRLDALSAELVRRYRDGEASVDGLLADD"
     assembly_gap    2306..2461
                     /estimated_length=156
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2596..3282
                     /locus_tag="PLV68_05020"
     CDS             2596..3282
                     /locus_tag="PLV68_05020"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRE00637.1"
                     /translation="MQSPSARTTQTAGTRLLVIRHGESEWNAARRWQGQADVELNERG
                     RLQAAEAGLNLGMFDALWASDLVRARLTAEIIGAIIGIGPVQVDQRLRETDVGPWEGL
                     THDEVNAGWPGFLDTFRRPEGFEDYSVAAERMMAAFADIATACPGGEVLVVTHGGVIR
                     AVRRAVGNPAEPDIHIPNLGGCWFQVHPPAGHDDTARITAGEIVTLSTDVAGPAGWLR
                     DDATGTTTPL"
     gene            complement(3305..>3987)
                     /locus_tag="PLV68_05025"
     CDS             complement(3305..>3987)
                     /locus_tag="PLV68_05025"
                     /inference="COORDINATES: protein motif:HMM:NF016430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRE00638.1"
                     /translation="ELYEKYADDLIRFATTVTADPATAEDLVSETVARMLARPDWSGV
                     ANPRSYLFTMLLNIARSQHRSRGRRLERERRIATAESGAWLSIDGLSAERLDVLRAVQ
                     QLSPTQRALLFCIHWDGTSVAEAAAHSVDRPKPCPSVVNRASAERGLAGIRRVHTPRQ
                     PNSTGAVVGVEVKASERVEGKHLTGLRRLRDRLGSAFVAGLAFHLGPVGYEAEDRIHS
                     LPIERLWR"
     assembly_gap    3988..4019
                     /estimated_length=32
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4084..>4331
                     /locus_tag="PLV68_05030"
     CDS             4084..>4331
                     /locus_tag="PLV68_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00639.1"
                     /translation="MGTEKRERQKANRQLKLEEMAREARKRKSRRRGLQIGIGVPLAV
                     VIVFGLVWLFGDSDDSKTATTTTTTAVTTTTVAPEVWP"
     assembly_gap    4332..4506
                     /estimated_length=175
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4507..4689
                     /locus_tag="PLV68_05035"
                     /pseudo
     CDS             4507..4689
                     /locus_tag="PLV68_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002525071.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
     gene            4706..4864
                     /locus_tag="PLV68_05040"
     CDS             4706..4864
                     /locus_tag="PLV68_05040"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRE00640.1"
                     /translation="MRPPNYTLFGQVTAGLDVVEAMNQLGVAESTPMGQQQPTEPITL
                     DSITITES"
     gene            4883..5524
                     /locus_tag="PLV68_05045"
     CDS             4883..5524
                     /locus_tag="PLV68_05045"
                     /inference="COORDINATES: protein motif:HMM:NF015534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HRE00641.1"
                     /translation="MTGTIALLGGGPFVANDELDARLIETAQATEVVVLPTADAFEHP
                     SRLIETAQAWGERLGVSVDGRLVLRRADANQTDHAAAVYNAKMVYLAGDSPLHLRSVL
                     KDTALFDALSHVLASGGVVAATGASAAALCDPMTDPRGGAFSLGLGLVNGLAVLPEVE
                     LMSPERLHRTLALATGFAVAVLPTGAALLGTSDGWERVGKAEVHGDLATVGIR"
     gene            complement(5550..5672)
                     /locus_tag="PLV68_05050"
     CDS             complement(5550..5672)
                     /locus_tag="PLV68_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00642.1"
                     /translation="MAELIDGLTDAHGSVANYVVTLGVRRSSIDSLRRRLIDAG"
     gene            5731..6075
                     /locus_tag="PLV68_05055"
     CDS             5731..6075
                     /locus_tag="PLV68_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00643.1"
                     /translation="MTAIALDALLVGPRRPRGAHPHHRVGGLAVVGSDVVTPHPDAGQ
                     QQRGQQPGAILASGAVEHHRRGVGRGDDLQPGGQLVRSGHEHVAVGPQQELVGGGELG
                     HLARMPGAVDDR"
     gene            6110..>6316
                     /locus_tag="PLV68_05060"
     CDS             6110..>6316
                     /locus_tag="PLV68_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00644.1"
                     /translation="MLDRAEAFALGKLDVGAQVDHRAKAAVAQHGDVAFGQAVQAIGA
                     VDAVPAGDATIDHRIAAEVTEVVHP"
BASE COUNT          929 a         2015 c         1995 g          843 t
ORIGIN      
        1 cgagccgcaa caggctgcgg aagcacagct cggtggtggc gtagagcgag ccgatcaccc
       61 ggtcgagctc gtcttcgcac aacaccccga ggggcagatc gccgcgcagg aacaccgcgt
      121 cttcgacgcc gatcgcgaag tgatgaccga tcagttgctg gttgcggcgc aacaggttct
      181 cgtagaccgc ggccacgttc tcttcgggcg cgggcatgac gtaggcctcg tactgcagcg
      241 tgcgctggcc gagcgtgagc cacacggtga tgaactcctt ctcatcgccg cgcatccgca
      301 cgtaccaccg cgcggtggcg gggtctcgat cgaccgcggc gatcgccggg ttcgtgtcgg
      361 cgaagcccgt cagccacgcg tcgatcgcgg cggccatcgc gccgacggag tcgtccgggt
      421 ggagctcgct catgagcagg cggccaggct gtgcaccgcc aggtcggcat agacccgatc
      481 gaggcagcca gcagccgtgc cccacgtgta gcgccgtgcc cgctcggcgc cgctcatgcc
      541 catcgacgtc gccagcgtgg ggtcgtcgag gatcgctgcg atgcgcgccg cataccggtc
      601 ggggtcacgt ccttcgacca ggtagccgtt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn nacctggctc gcgacgccca gctcgtcgat caaggcatgg
      841 acgcgctgca cctcgctacc gccttccagc ccgctcgctc cgccgaccac gaccagacgg
      901 gcgtcggggc ggccgagcgc ggccagcgcc cgcaccgcga cgtcgacccc cttcaacggc
      961 tgaatacgcc cgacgaacaa caacaccgga cccggtccca taccgagcgc gcgtcgggcg
     1021 ccgccctgat ggcccggggc gaacagcgcg tgctcgacgc cgggcgccac gatctcgatc
     1081 cgcccgggcg ggtcgccgta gaggcggcgg aactggcgct cctcttcggt gcagctgacg
     1141 cagatgccgt cggcgcagtt gatgatctcg gcctcggccc gctcgcgcca cagcggctcg
     1201 ggatcgccgc cttcggcctt gacccgcgcc agcgtgtgga acgtcgacac gaatggcacg
     1261 tcgagttcgt gcttgatgcg atgtgcgacc aacccactga gccagtagtt ggcgtgcagc
     1321 acgtcggccc ccgggccgtc gtgcagatcc tcgatcaccc ggtcgccgaa ctcgtcgacc
     1381 acgtgatgca ggtcgtcctt ggccagatcg aacgacccgg ccgggatgtg gaccacgcgg
     1441 tggcccggct cgatgtcgac cacacggggt tggtcgggcc ggctggcgcg ggtgtaggtg
     1501 gtgcactcga cgccgcgctg ggccagcgac gacaccagtt cgcgcacgta cacgttcatg
     1561 ccgccgctgt cgccagagcc aggctgggcg agcggggagg tatgaagcga gagcactgcg
     1621 acccgttgca cgatggctga aaccctaccg agccagtcgt cattcccgct gaccgagtgc
     1681 atccgctcgg agacgggatt cagtcgtcgg cgagcaggcc gtcgacgctg gcctcgccgt
     1741 cgcggtagcg ccgcaccagc tccgcgctga gcgcgtcgag gcggccgaac cgcttgtcca
     1801 cctggcgctc gtgcgagcgc tcctgctcga agctggccag ttcggtggcc accgcgtcga
     1861 gttgctcgtc gctcaactcg tcgagccggt cggcacccag gcggcgacac agctcgtcga
     1921 gctcgatcgc cagcggatga tcgctggcat cggcagtcgg gcgcggggga cgagccgggc
     1981 caccggtgag atgacggccc agcacagcgg gcaggtcctc gtcgacgttg aactgcgtgc
     2041 cggccagacg gtgcttgcgc tcgagctcga ccatgtcgag acgcgcctgc accaaacggc
     2101 gcacgtagct gacggcatcg tcggctgcct gcaacgtggc ccgttcggcg cgcaggtcgg
     2161 ccaacgacaa ctcggaggga tcgagcgtgg gtgtggtctg agcagacatc agatctcctt
     2221 ctgcgaactg gaacaggtcc gactggccgc cggacgagat ggcggacgag atgccgaacg
     2281 aggtgccggc gcgaacgcgc cacctnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nggccgacgg tggcgccggg caccacgtcg cgcccgacac cagcggcctc gcgggccgaa
     2521 gcgaacaggc gcagagtagg cacaacgcgc atcgtaccgt tgcgccgcga acgcgtgggg
     2581 ctaccctcgc ccgccatgca gtctccatcc gcccgcacga cccagacggc agggacgcgg
     2641 ctgctggtga tccgccacgg cgagagcgaa tggaacgcgg cccgccggtg gcagggccag
     2701 gccgacgtcg agctcaacga acgaggtcgc ctgcaagcgg ccgaggccgg gctcaacctg
     2761 ggcatgttcg acgccttgtg ggccagtgat ctggtccggg ctcggctgac ggctgaaatc
     2821 atcggggcga tcatcgggat cggtccggtg caggttgacc aacgtttacg agaaactgac
     2881 gttggcccgt gggaaggcct gactcatgac gaagtcaacg cgggatggcc gggattcctt
     2941 gacacgttcc gccgcccgga gggtttcgag gactactccg tcgcggccga gcgaatgatg
     3001 gctgcgttcg ccgacatcgc cacggcctgc ccaggcggcg aggtgttggt ggtgacccac
     3061 ggcggcgtca tccgcgccgt gcgccgcgcc gtcgggaacc ccgccgagcc cgacatccac
     3121 atccccaacc tcggcggctg ctggttccag gtccaccccc cggccgggca tgacgacacc
     3181 gcacggatca ccgcgggcga gatcgtcaca ctctccaccg acgtcgccgg cccagcagga
     3241 tggctccgcg acgatgcaac cggcaccacc acccccctct agaccccagc aggctcgcct
     3301 ggcgtcaccg ccacagtcgt tcgatgggca gcgaatggat gcgatcttcg gcctcgtagc
     3361 cgaccggacc gaggtgaaac gcaaggcccg ccacgaacgc cgatcccagt cgatcacgca
     3421 gccgcctgag ccccgtcagg tgcttcccct caacccgctc actcgctttg acttcgacgc
     3481 caacgacggc gcccgttgaa ttcggctgcc gcggagtatg gactcgccgg attccggcca
     3541 atccccgctc cgcggaagcc cgattcacca ccgatggaca cggcttcggg cggtctactg
     3601 agtgggcggc ggcttcggcg acggacgttc cgtcccaatg gatgcagaac agcagtgctc
     3661 gctgggtcgg ggacagctgc tgcaccgcac gcagcacgtc gagccgctcg gctgacaggc
     3721 cgtcgatact gagccaggcg ccgctctccg cggtggcgat acggcgctct cgttcgagac
     3781 ggcggccgcg gctgcggtgc tgagaacggg cgatgttgag caacatcgtg aacaggtacg
     3841 agcgcgggtt ggcgacgccc gaccagtcgg gccgggccag catccgggcg acggtttcgg
     3901 atacgaggtc ttcggccgtt gccgggtcgg cggtgacggt ggtcgcgaag cggatcaggt
     3961 cgtcggcgta cttctcgtac agctcttnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna
     4021 tgccgcggga acggggagtg cgccacatct ggtgggggga tcggcaggta gcctctcggc
     4081 cctgtgggta ccgaaaagcg tgagcgccaa aaggccaacc gtcaactgaa gctggaagag
     4141 atggcccgcg aggcccgcaa gcgcaagtcc cgccgccgcg gactgcagat cggcatcggt
     4201 gtaccgctcg ccgtggtgat cgtgttcggg ttggtctggc tgttcggcga cagcgacgac
     4261 agcaagacgg caaccaccac cacgaccacc gccgtgacga ccacgacggt cgcacccgag
     4321 gtctggccgt gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4501 nnnnnnggcg tcgcctgcca ccgcgtgatc aaggacttcg tggcgcaatg cggcgaccca
     4561 accggcaccg gctcgggtgg ccccggctac gagttcgcgg acgaactgcc cgaagcaggc
     4621 gagtacacga tcggctcgct ggcgatggcc aactcgggcg tcaacaccaa cggttcgcag
     4681 ttcttcatca tcaccggcga caacggtgcg gccgcccaac tacacgctgt tcggtcaggt
     4741 cacggccggg ctcgacgtgg tcgaggcgat gaaccagctc ggcgtcgccg agagcacacc
     4801 gatgggtcag cagcagccca ccgagccgat caccctcgac tcgatcacga tcaccgaaag
     4861 ctgatccgga aggcggcatg gcatgactgg aacgatcgct ctcctcggtg gcggcccgtt
     4921 cgtcgccaac gacgaactcg acgcccgcct gatcgagacg gcccaagcga ccgaagtggt
     4981 cgtgctgccg accgccgacg cgttcgagca cccgagccgg ctgatcgaga cggcgcaggc
     5041 gtggggcgag cgcctcggcg tcagcgtcga cggacggctg gtgttgcgcc gcgccgacgc
     5101 caaccagacc gatcacgccg cagccgtgta caacgccaag atggtctacc tcgcgggcga
     5161 ctcaccgctg cacctgcgct cggtcttgaa ggacaccgcg ctgttcgacg cgttgagcca
     5221 cgtgctggcc agcggcggtg tcgtcgcggc cactggggcc agcgccgcgg cgctgtgcga
     5281 cccgatgacg gatccccgcg ggggtgcctt ctcgctcgga ctcggcctgg tcaacgggct
     5341 ggccgtgcta cccgaggtgg agctgatgtc gcccgagcgg ctgcatcgca ccctcgccct
     5401 ggcgacgggg tttgcggtgg cggtgctgcc gaccggcgcg gccctgctcg gcacgtccga
     5461 cggttgggag cgggtcggca aggccgaggt ccacggcgac ctcgccaccg tcggcatccg
     5521 ctgacacccg ccccgctctc ggcgtcgcgt cagcccgcgt cgatgaggcg gcggcggagc
     5581 gagtcgatgc tcgaccggcg cacgcccaac gtgaccacgt agttggcaac gctgccgtgg
     5641 gcgtcggtca gcccgtcgat cagttcggcc atcaccgtgg ctggggactc gaagtagtgc
     5701 cggcggtcgt cctcggcggc gatcgaggtc gtgaccgcga tcgcgctcga tgccctgctc
     5761 gtcggcccac gccgcccacg cggcgcgcat ccgcaccatc gcgtcggcgg tcttgccgta
     5821 gtcggcagcg acgtagtcac gccccacccc gacgctggcc agcaacagcg cggccagcag
     5881 cccggtgcga tccttgccag cggcgcagtg gaacaccacc ggcgtggcgt cggacgcggc
     5941 gatgatctcc aacccggcgg ccaacttgtc cgatccggcc acgagcatgt cgcggtaggc
     6001 ccacagcagg aactcgtggg cggtggcgaa ctcgggcacc tcgcccggat gccaggtgcg
     6061 gtcgacgacc ggtaggtgat ggaaggtcac gacgtgccgg tcgcttcaga tgctcgaccg
     6121 cgcggaagcc ttcgcgctcg gcaagctcga cgtcggtgcg caggtcgatc accgtgccaa
     6181 ggccgcggtc gcgcagcacg gcgatgtcgc gttcggtcag gcggtgcagg ccatcggcgc
     6241 ggtagacgct gttccagcgg gtgatgccac catcgaccac cggatagccg ccgaggtcac
     6301 ggaagttgtg cacccc
//
LOCUS       DAORNF010000090         6309 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_9157_length_6309_cov_1.616885, whole genome shotgun sequence.
ACCESSION   DAORNF010000090 DAORNF010000000
VERSION     DAORNF010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 6309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6309
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9157_length_6309_cov_1.616885"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            130..>316
                     /locus_tag="PLV68_05065"
     CDS             130..>316
                     /locus_tag="PLV68_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00645.1"
                     /translation="MPISPDSPASVPAATPEIGIVVLHGFGGTAASVAPLTDALRAAG
                     HPVVSPTLPGHGTTTDDL"
     assembly_gap    317..353
                     /estimated_length=37
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            402..>750
                     /locus_tag="PLV68_05070"
     CDS             402..>750
                     /locus_tag="PLV68_05070"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00646.1"
                     /translation="MGGTLALDAARRRGDVVGVVAINAIGVVADPDVIEHLEYMIGRG
                     KTMQPAGAPDIRNPDARDSAYIEQPRQALLEMTIGAGAVSARLGEVSVPVLVVSGDHD
                     ATIEPANSDAIAAA"
     assembly_gap    751..848
                     /estimated_length=98
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1097..1246
                     /locus_tag="PLV68_05075"
     CDS             1097..1246
                     /locus_tag="PLV68_05075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00647.1"
                     /translation="MTRLGINSFDATATAGRKGLPYDWQSLLAAEGVAEIEAELSELV
                     LEPSE"
     gene            complement(1270..1572)
                     /locus_tag="PLV68_05080"
     CDS             complement(1270..1572)
                     /locus_tag="PLV68_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00648.1"
                     /translation="MTETTATTEATEMTEATVVGPPSTKAQIIPDDELAPQLLDMFIQ
                     NFGDVNFAGCVHQQVFDALARGEIATVDIANYIRNLVTGPMQAALVSIQDAATCRR"
     assembly_gap    2032..2086
                     /estimated_length=55
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2087..>2389)
                     /locus_tag="PLV68_05085"
     CDS             complement(<2087..>2389)
                     /locus_tag="PLV68_05085"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HRE00649.1"
                     /translation="TKTVSVAEVTVGGSATFTLTAANIGTGPATGVVAVDTLPVGMTP
                     TLLPAGMTYNAGTRQLTWTIGDLAAGASVSVEYTVTVSSAGTLVNVVVVTSETPGDP"
     assembly_gap    2390..2445
                     /estimated_length=56
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2446..>3605)
                     /locus_tag="PLV68_05090"
     CDS             complement(<2446..>3605)
                     /locus_tag="PLV68_05090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00650.1"
                     /translation="EPFGPAIVVDGAVRWTYAVTNTGNVHLADVTVVDDQGVVISCGV
                     ADADQDGDIDLLAPGAVVTCVGNGISESGQYENLATVQGTPADPDGTPLVDGDGDPLD
                     KPTDDDPSHYFGADPGISIVKYTNGEDADTPTGPFVLTNRTVTWTFEVTNTGNTALLA
                     ATVTDDKVPAAAINCGDGSNVIALMLPGDVVTCTATGIAIAGQYANLGSVTAAPALPA
                     FDDGIDPDDPDTWPSDPDDYEPIEYTDVDGGTTTAPPVTDDDPSHYYGMNPAIDLVKE
                     VCLIADQNGCDPANNAHWGKFVVVTSGTLAHYRITVTNTGSVDLAPVDITDPAVPNCA
                     TTIASLAIGASHRYTCTLDPVPGALHNVATAVGNPVDKDGEDIIDPDTHQPIP"
     assembly_gap    3606..3654
                     /estimated_length=49
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3655..4584)
                     /locus_tag="PLV68_05095"
     CDS             complement(<3655..4584)
                     /locus_tag="PLV68_05095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00651.1"
                     /translation="MVTGPITLSATLSSTASAQIPGNEPTNGYTNNTLDLALLGAPAI
                     QIIKEVCDPTAGDCDAGADLGGDGWDDVSYDADFLEDITWRITVTNTGWQTLTDVKVN
                     DPLVAACATPVAFTPPASLTPGSSFSYTCETPDLIVGFVNTADVEGTGPTGTKVTEET
                     SPETAKIQAEVTTPDPNPGIMVKKYVVNIAYPADEHDAQTAPGLYVPVGDTVTWVYDV
                     RLADGKRVPLKDVTLTDDGGPNPSFSGTYLSGDTNGNNLLEYGETWRFTTTSQLVVTA
                     GQYTNVATAQGTPVTPEAEPISATDPANHYGVQA"
     gene            complement(4481..>6309)
                     /locus_tag="PLV68_05100"
     CDS             complement(4481..>6309)
                     /locus_tag="PLV68_05100"
                     /inference="COORDINATES: protein motif:HMM:NF028519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HRE00652.1"
                     /translation="GPMRVIEVFPYNGDAAVPSGPNRNPASAYHGKLDLTALIVPKYP
                     NNTDVPGTMYYSADAPNTISQDWNAHVDGGYPTTWCTTADGVTWTVVHGAGSCPTTLA
                     DVTAFMFLQGNALGVVSSPNTSRVNIPFTLQAGDTVDPFGADANRPGDNYTNRFTGQV
                     PQILKDGQPQRLDSNTVRVKVVGYSVGDWLWFDRDDDGIYDAAVDFVVPDGTVVELYS
                     VGPDGVVGGGDDVLLGSTSTTGGQYLFTDLPDGMVYVQVPASEFQAGGHLYGFRISNA
                     AAGVEDENDDVSHDAVAGTGGLAVGGAISTVHTLQAPVLVNGQPVGSEPSGDNLHGIV
                     DPTTTDRFSNLTVDLAFNGLGSIGDRVWYDVNRDGVQDAGEPGIQNATVTVTWFGPDG
                     VLGGGDDIVFPAQTTDGNGIYGVSNLPYGKYQVAVAALDPDFGPTFDLDGIGTPHVAV
                     AELTPAVPNRTDVDFGYSVAFTLGNKVWFDSNNDGNFDVGERNDRAAVERDHQHVGHL
                     HHHNRWQLSVRELARRRLLRGHPGSRVHRSAHGRGGVDQRRRQPQQRCGRDHRPQRGG
                     GQRFVRGSGGHRPDHAVGDALVHRQRADPRQRADQRLHQQHS"
BASE COUNT          913 a         1962 c         2110 g         1029 t
ORIGIN      
        1 cgccggacga gttggccacc gcgggcatca caccgggtct gctgcgggtc tcgctcggcc
       61 tggaagaccc cgacgacctg atcgccgact tcgtccaagc gatcgacgcg ccctacagct
      121 gagccgccgg tgccgatctc acccgactct ccggcatctg ttcctgcggc cacgccggag
      181 atcggcatcg ttgtgctgca cggcttcggt ggcacggcgg caagcgtcgc cccactgacc
      241 gacgcgctgc gcgccgccgg tcatccggtg gtgtcgccga cactgcccgg ccatggcacg
      301 accaccgacg atctggnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnggcgggt
      361 caggcggtgc cggtcgccgg gtggtcgtga tcgggcagtc gatgggggga acgctggcgc
      421 tcgatgcagc acgacgacgc ggcgacgtcg tcggcgtcgt cgcgatcaac gcaatcggtg
      481 tcgtggccga ccccgacgtg atcgaacacc tggagtacat gatcgggcgc ggcaagacga
      541 tgcagcccgc gggggccccc gatatccgca accctgatgc gcgcgactcg gcctacatcg
      601 aacaaccgcg ccaggcactg ctggagatga cgatcggagc cggcgccgtc agcgcccgac
      661 tcggcgaggt gagcgtgccg gtgttggtcg tgtcgggcga ccacgacgcc acgatcgaac
      721 cggccaactc cgacgccatc gccgctgcgg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      841 nnnnnnnncg gccctctgaa ccctcccgct cactggccgg agagtgagtg cgtctgacgg
      901 atcgatcagg cgtcgctggc tgatgaatca gcgagtagcg gtcggggtct gggctcagta
      961 ttttggcgcc cgctgcgcgg acccgagaga cccagaggcc gggcagaaag gacaccgatc
     1021 accgccacgc cggatacacc gacccggctg gcacccccac ctccgatctt cgcaacgtcg
     1081 cgaaaggacc gccaccatga ctcgccttgg catcaacagc ttcgacgcaa ccgcaaccgc
     1141 cggccgcaag ggcctcccct acgactggca gtccctgctg gccgccgaag gtgtggccga
     1201 aatcgaagcc gagctgagcg agttggtcct cgaaccatct gagtgagtgt ggctgagtga
     1261 accgccacct cagcggcggc aggtcgcggc gtcctgaatg ctgaccaacg cggcctgcat
     1321 cggcccggtc accaggttgc ggatgtagtt ggcgatgtcg accgtggcga tctcgccgcg
     1381 ggcgagcgcg tcgaacacct gctgatgcac gcaaccggca aagttgacat cgccgaagtt
     1441 ctggatgaac atgtcgagca actgcggcgc gagttcgtcg tcgggaatga tctgggcctt
     1501 ggtcgacggt ggccccacga ccgtcgcctc ggtcatctcc gtcgcctctg tcgttgccgt
     1561 cgtctcggtc atctccgtcg cctcggtcgt ctccgtgccg gcgaggctgg aatcggtcgt
     1621 gggcagagcg gtcgtcgatg actcggcaac ccctgtgccg gcaccgccgg acggtgccag
     1681 gcggtgccga cgcagcggct gccgagtcat cgtcactgga gcaggcgccc aacccgagtg
     1741 cggcgagacc gagcgatgcg ccgagggcga acccagctcg cttgcgcagg tggacaccgc
     1801 ccccgtcacg tctcggggac accgacggtc gtgccgagta ttgggccagg tgttgtgccg
     1861 ggtgatgtgg tggtgtcacc cctcgggagg caacaccacc acgctctcgc ctgatcaagc
     1921 gctgcgccgt cgtcggcggg tgccgccgag caggcccacg ccggccaggg tgaccagtcc
     1981 ggccatccac agcaacggac cggaagcggt accggtcacc acgattggcc gnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngggt cgcccggcgt
     2101 ctccgacgtg acgacgacga cgttgaccaa cgtgccggcg ctcgacaccg tgacggtgta
     2161 ttcgaccgac accgacgcgc ctgcggcgag atcaccgatg gtccacgtga gctgccgcgt
     2221 cccggcgttg taggtcatgc ccgcgggcag caacgtgggg gtcatgccca ccggcagggt
     2281 gtcgaccgca accacgcccg tggccggacc agtaccgatg ttggccgcgg tcagcgtgaa
     2341 ggttgcagaa ccgccgacgg tcacctcggc gacgctgacc gtcttggtgn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngggat cggctggtgg
     2461 gtgtccggat cgatgatgtc ctcaccgtcc ttgtccaccg ggttgccgac tgccgtggcg
     2521 acgttgtgca gggcacccgg caccgggtcg agggtgcagg tgtatcggtg cgacgctccg
     2581 atcgccaacg aggcgatcgt ggtggcgcag ttcggcacgg ccggatcggt gatgtcgacc
     2641 ggcgccagat cgaccgagcc ggtgttggtc accgtgatcc ggtaatgggc cagcgtgccg
     2701 ctggtgacca ccacgaactt gccccagtgg gcgttgttcg ccggatcgca accgttctgg
     2761 tcggcgatca ggcagacctc cttgaccagg tcgatcgcgg ggttcatgcc gtagtagtgc
     2821 gacgggtcgt cgtcggtcac cggcggcgcg gtggtggtac cgccgtcgac gtcggtgtac
     2881 tcgatcggct cgtagtcgtc tgggtcggac ggccacgtgt cgggatcgtc aggatcgata
     2941 ccgtcgtcga acgccggcag cgccggggcg gccgtgaccg aaccgaggtt ggcgtactga
     3001 ccggcgatcg cgatgcccgt cgccgtacag gtgaccacat cacccggcag catcagtgcg
     3061 atcacgttgc tgccgtcgcc acagttgatc gcagcggccg gcaccttgtc gtcggtgaca
     3121 gtggccgcga gcaacgccgt gttgccggtg ttggtgacct cgaaggtcca cgtcaccgtg
     3181 cggttggtga gcacgaacgg cccggtcggg gtgtcggcat cctcgccgtt ggtgtacttg
     3241 acgatcgaga tgccgggatc ggccccgaag tagtgcgacg ggtcgtcgtc ggtcggcttg
     3301 tccagcgggt cgccatcacc gtcgaccaac ggggtcccgt cggggtcggc gggtgtgcct
     3361 tgcaccgtgg ccaggttctc gtactggccg gattcggaga tgccgttgcc gacgcaggtc
     3421 accaccgcac ccggagcgag cagatcgatg tcgccgtctt ggtccgcatc ggccacgccg
     3481 caggagatga ccacaccctg gtcgtcgacc acggtgacgt cggccaggtg cacgttgccg
     3541 gtattggtca cggcgtaggt ccagcgcacc gcgccgtcga cgacgatcgc cgggccgaac
     3601 ggctcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncgcctg
     3661 cacgccgtag tggttggccg ggtcggtcgc cgagattggc tcggcctccg gcgtcaccgg
     3721 cgtgccctgc gcggtggcga cgttggtgta ctgccccgcc gtgaccacca actgggacgt
     3781 cgtggtgaaa cgccaggtct cgccgtactc caacaggttg ttgccgttgg tgtcgcccga
     3841 caggtaggtg cccgagaagc tcgggttggg cccgccgtcg tcggtgaggg tcacgtcctt
     3901 gagcggcacc cgcttgccat cggccaggcg gacgtcgtac acccaggtga ccgtgtcgcc
     3961 gaccggcacg tacagaccgg gagcggtctg cgcgtcgtgc tcgtcggctg ggtaggcgat
     4021 gttgaccacg tacttcttca ccatgatgcc cgggttcggg tcaggcgtgg tgacctcggc
     4081 ctggatcttt gcggtttcag gcgaggtctc ctcggtgacc ttggtgccgg tcggcccggt
     4141 gccttcgacg tcggcggtgt tgacgaagcc cacgatcagg tcgggggtct cgcaggtgta
     4201 cgagaacgac gaacccggcg tcagcgaggc cgggggcgtg aacgccaccg gagtcgcgca
     4261 cgccgccacc agcggatcgt tgaccttcac gtcggtcagc gtctgccaac cggtgttggt
     4321 gaccgtgatc cgccacgtga tgtcttcgag gaagtcggcg tcgtaggaca cgtcgtccca
     4381 gccgtcgcca cccaagtcgg cgcctgcgtc acagtcaccc gctgtcgggt cacagacctc
     4441 cttgatgatc tggatcgcgg gcgcacccaa cagagccagg tcaagagtgt tgttggtgta
     4501 gccgttggtc ggctcgttgc cggggatctg cgcgctggcg gtggacgaga gcgtcgccga
     4561 cagcgtgatc gggccggtga ccaccgctcc cgcgaacgaa ccgctgcccg ccaccgcgtt
     4621 gtggtcgatg gtctcgtcca catcgttgtt ggggttggcg acgccgttga tcgacgccac
     4681 cgcgcccgtg agcggaccgg tgaacgcggc tgccgggatg accacgaagt agtcgccggc
     4741 gggcaagttc gcgaactgat agctgccacc ggttgtggtg gtggaggtga ccaacgtgtt
     4801 ggtggtcgcg ttccacagct gcacggtcgt tccgttcacc gacgtcgaag ttgccgtcgt
     4861 tgttgctgtc gaaccacacc ttgttgccca gcgtgaacgc aaccgagtag ccgaagtcga
     4921 cgtcggtgcg gttggggacg gcaggcgtca gctcggcaac ggcgacgtga ggtgtaccga
     4981 tgccgtcgag gtcgaacgtg gggccgaagt cgggatccag cgcagcgacc gcaacctggt
     5041 acttgccgta cggcaggttg ctcaccccgt agatgccgtt gccatcggta gtctgcgcgg
     5101 ggaagacgat gtcgtcgcca ccgccgagca caccgtcggg gccgaaccac gtgaccgtga
     5161 cggtcgcgtt ctgaatgccg ggctcgccag catcttgcac accgtcgcgg ttgacgtcgt
     5221 accacacccg gtcaccgatc gagcccaggc cgttgaaggc cagatcgacg gtgaggttgc
     5281 tgaacctgtc ggtggtggtc gggtcgacga tgccgtgcag gttgtcaccc gacggctcgc
     5341 taccgaccgg ctggccgttg acgaggaccg gcgcttgcag cgtgtgcacc gtgctgattg
     5401 caccaccgac ggcgaggcca ccggtgccag cgaccgcgtc gtgcgacacg tcgtcgttct
     5461 cgtcctcgac gcctgcagca gcgttggaga tgcggaagcc gtacaggtgg ccgccggcct
     5521 ggaactcgct ggccgggacc tgcacgtaca ccatgccgtc gggcagatcg gtgaacaggt
     5581 actggccgcc cgtggtgctc gtggagccga gcagcacgtc gtcaccgccc ccaaccacgc
     5641 cgtcggggcc gacgctgtac aactcgacca cggtgccgtc gggcaccacg aaatcgaccg
     5701 ccgcgtcgta gatgccgtcg tcgtcgcggt cgaaccacag ccagtcgccg accgagtagc
     5761 cgaccacctt gacgcgaacg gtgttcgagt cgaggcgctg tggctggcca tccttcagga
     5821 tctgcgggac ctggcccgtg aagcggttgg tgtagttgtc gccaggccgg ttggcgtcag
     5881 cgccgaacgg atcgaccgtg tcgccggcct gcagcgtgaa ggggatgttg acccggctgg
     5941 tgttgggcga gctgaccacg ccgagggcgt tgccctgcag gaacatgaac gccgtcacgt
     6001 cggccagcgt cgtcggacag cttccggcgc cgtggacaac cgtccaggtg acgccatcgg
     6061 ctgtggtgca ccacgtggtc gggtacccac cgtcgacgtg cgcgttccag tcctggctga
     6121 tcgtgttcgg cgcgtcggcc gagtagtaca tcgtgccggg cacgtcggtg ttgttggggt
     6181 acttcggcac gatcagcgcg gtcaggtcga gcttgccgtg gtaggcgctg gccgggttgc
     6241 ggttgggacc gctcggcacc gcggcgtcgc cgttgtaggg gaacacctcg atgacgcgca
     6301 tcggcccga
//
LOCUS       DAORNF010000091         6307 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_9165_length_6307_cov_1.599968, whole genome shotgun sequence.
ACCESSION   DAORNF010000091 DAORNF010000000
VERSION     DAORNF010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 6307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6307
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9165_length_6307_cov_1.599968"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="PLV68_05105"
     CDS             complement(<1..262)
                     /locus_tag="PLV68_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00653.1"
                     /translation="MTAALTTTVAALLVDVARRNRRGHAIALTKRTGRNTELAKLGAA
                     VGVTYATTSAPAAAIFAIMTKVGAYAIIRVSTLIFGAGAGPLA"
     assembly_gap    356..492
                     /estimated_length=137
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<493..>717)
                     /locus_tag="PLV68_05110"
     CDS             complement(<493..>717)
                     /locus_tag="PLV68_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00654.1"
                     /translation="ASSRQERLGFVGIGGLAALSRLTPAQSRQWLTQRLYLQRKAAEW
                     QPWAVQEANRHEWRMVLEAGRAIGNFSSRS"
     assembly_gap    718..757
                     /estimated_length=40
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<758..1009)
                     /locus_tag="PLV68_05115"
     CDS             complement(<758..1009)
                     /locus_tag="PLV68_05115"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRE00655.1"
                     /translation="MLLHGWTASADLNWFLTYFPIGRDYRVVAIDHRGHGRGIRSRRA
                     FRLADCADDAVALADELGIERFIPIGYSMGGPIAQLVWHR"
     gene            complement(1189..1668)
                     /gene="greA"
                     /locus_tag="PLV68_05120"
     CDS             complement(1189..1668)
                     /gene="greA"
                     /locus_tag="PLV68_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HRE00656.1"
                     /translation="MATHQFSRAAYDRMQAEFDDLTTRGRFAVAEKIERARELGDLRE
                     NGDYHAAKDEQGHMEGRIRELEYLLENSEIVEDAPAGVVQVGNKVTVHYEGETEADAT
                     TYIVGHIEELIDGYDLISPGSPLGSTLIGATVGSWVSFKAPNGELKIKIVSAEAAHP"
     gene            1758..>2370
                     /locus_tag="PLV68_05125"
     CDS             1758..>2370
                     /locus_tag="PLV68_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HRE00657.1"
                     /translation="MTHRIALIGGDGIGPEVTAVAVQVVRAAGVGIETTDFDLGGSRY
                     LRDGYVLSDATVDQLREFDAILLGAVGTPEVKPGVIERGLLLKMRFDLDLYVNQRPFV
                     GVGPGHTVGHEFVVIRENTEGPYVGEGGVLRKGTPHEVATQGSVNTRFGVERVIRYAF
                     ELAATRERKHVTLVHKTNVLSFAGDLWQRTFDDVAAEYPGVGTA"
     assembly_gap    2371..2435
                     /estimated_length=65
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2436..2754
                     /locus_tag="PLV68_05130"
     CDS             <2436..2754
                     /locus_tag="PLV68_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HRE00658.1"
                     /translation="VTDNLFGDILTDLGGVVSGGIGLAASANLNPSRTGPSMFEPVHG
                     SAPDIVGTGKANPVAAILSAALMLDFLGETAAAAKVRAACEDPGTGTTTEIGDRIAAR
                     VAG"
     gene            2770..>3187
                     /locus_tag="PLV68_05135"
     CDS             2770..>3187
                     /locus_tag="PLV68_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HRE00659.1"
                     /translation="MPIQTTPKIWMNGELVDWDKAQVHVLTHTLHYGTGVFEGIRAYA
                     TADGPAVFRLTDHIKRLFNSAKIVGMEIPYTVDEIVEATKQTVASTGLDACYVRPLAY
                     YGYGEMGLNTLPCTTDVAIACWPWAPFLGHEAVRMKT"
     assembly_gap    3188..3284
                     /estimated_length=97
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3285..3677
                     /locus_tag="PLV68_05140"
     CDS             <3285..3677
                     /locus_tag="PLV68_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HRE00660.1"
                     /translation="KAGYDEAIMLAPNGLVAECTGENIFAVRNGIIITPPLSAGALEG
                     ITQSSVITIARDLGMDIRVDNLARSDLYIAEEVFVCGTAAEISSVRSIDDREIPANGP
                     ITNAISEVYLKAVRGEDDRYKHWCELAV"
     gene            complement(4195..4956)
                     /locus_tag="PLV68_05145"
     CDS             complement(4195..4956)
                     /locus_tag="PLV68_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HRE00661.1"
                     /translation="MNRPSPDHTASAAPTFAPASASADTDPDTQAASPTWPAAVLWDL
                     DGTIVDTEPYWMQAEYDLVAQFGSEWNHHHAQAIVGFDLINAAEYIRAHAHVDLTPRA
                     IVDWLIERVEAQLDGAIPWRPGARELLAEVHAAGIPNVLVTMSWKRLTRPIVAALDPG
                     VFTAVIAGDDVPAGQGKPHPTPYRLGAAAAGVDPHDCLAIEDSPTGATSALAAGCTVL
                     GVPNVKAIDPAPGLTLHPTLANLTLPTLARLAPLA"
     gene            5163..>6307
                     /gene="cimA"
                     /locus_tag="PLV68_05150"
     CDS             5163..>6307
                     /gene="cimA"
                     /locus_tag="PLV68_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HRE00662.1"
                     /translation="MSTEPNPESHSRSLPTRVEVFDTTLRDGLQVEGVSATVEDKLRI
                     AEQLDFLGVHFIEGGWPGANPKDIEFFNRAAKELRFTTSRMVAFGSTRRPKGKVDDDA
                     TLRNLIEAGTDVVCIVAKSWDYHVLNALQTTLDEGEAMISDSVAFLAAHGKQVLVDME
                     HFFDGFKRNPEFALRAVEAAIIKGASHVVLCDTNGGSLPHEVLRIVGDVKAHIGDDAV
                     IGIHCHDDTGCAVANSMAAVLAGGGHVQGTLNGLGERTGNCNLTTVIPNLQLKLGIEC
                     LPEGRLERLTSVSNHIAEVLNRPLNPQAPYVGASAFAHKAGLHVSAIARAKDAYEHVD
                     PDLVGNSTRFVVSEMAGRATLNIKADELGISLDGPALNQVIDDLKRL"
BASE COUNT          963 a         2019 c         2017 g          969 t
ORIGIN      
        1 cggcgagggg gccggcgccc gcgccgaaga tcagcgtcga gacgcggatg atggcatagg
       61 cgccgacctt cgtcatgatc gcgaagatcg cggcggcggg cgccgacgtc gtggcgtagg
      121 tgaccccgac tgcggcgccg agcttggcca gctcggtgtt gcggccggtg cgcttggtga
      181 gcgcgatcgc gtgcccgcga cggttgcgtc gggcaacatc gaccaacagc gctgcgaccg
      241 tcgtcgtcaa cgctgccgtc acccaccatc gtttacgcat tcgccgcctc gcgcagaccg
      301 gcccgcgagc acaccgatgc gagcgcgtcc cgcaaggcag gcacgaactg gccggnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      481 nnnnnnnnnn nncaactccg cgacgagaag ttgccgatcg cccggcccgc ttcgagcacc
      541 atccgccact cgtgacggtt cgcttcctgc accgcccacg gctgccactc ggcggccttt
      601 cgttgcaggt acaggcgttg cgtcagccat tggcggctct gcgcgggggt caggcgtgac
      661 aacgcggcga gcccgccgat gccgacgaac cccaatcgct cttggcgact ggacgcgnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngcg gtgccacacc aactgcgcga
      781 tcggtccgcc catgctgtag ccgatgggga tgaaccgctc gatgcccaac tcgtcggcca
      841 aggcgaccgc atcgtccgcg cagtcggcga ggcggaacgc ccggcggctg cggatgccgc
      901 gcccgtggcc gcggtggtcg atggcgacga cccggtagtc gcgcccgatc gggaagtagg
      961 tgaggaacca gttgaggtcg gcgctggcgg tccagccgtg cagcaacatc accgtcggcg
     1021 cgcccggggg accggcgatc tcgcggaaga acgtgcggcc ccgcccgggc aggtcgatgt
     1081 cgcgcccgaa gggcaggatc ggtccgggtg gaccggactc tgtggcgctg gtgccagtgg
     1141 cgtcggtatc ggcatgcgat cagtgagcct tggtggccgt cgaggcggtc acgggtgggc
     1201 ggcttcggcg ctcacgatct tgatcttgag ctcgccgttg ggcgccttga agctgaccca
     1261 cgatccgacc gtggcaccga tcaacgtgct gcccaagggg gagccggggc tgatcaggtc
     1321 gtacccgtcg atcagttctt cgatgtgccc gacgatgtac gtggtcgcgt cggcttcggt
     1381 ctcgccctcg tagtgcacgg tgaccttgtt gccgacctgc accaccccgg ccggggcgtc
     1441 ttcgacgatc tcgctgtttt cgagcagata ctcgagctcg cggatgcgcc cctccatgtg
     1501 gccctgctcg tctttggcgg cgtgataatc gccgttctcg cgcaggtcgc cgagctcgcg
     1561 ggcccgctcg atcttctcgg ccacggcgaa ccggccacgg gtggtgaggt cgtcgaactc
     1621 ggcctgcatc cggtcgtatg cagcacggga gaactggtgt gttgccatct ggtgaatcta
     1681 tcggcaggac atcgcgggtt gctggcgtct cgacatgtgg gatcgcattc tttcagtgcg
     1741 ggaactacgc ttgccccatg acgcatcgca tcgcactcat tggtggagac ggcatcggcc
     1801 ccgaggtgac ggcggtcgcc gtgcaggtgg tgcgggcggc cggggtcggc atcgagacca
     1861 ccgacttcga cctcggcggc tcgcgatacc tgcgcgacgg ctacgtccta tccgacgcca
     1921 ccgtcgacca actccgcgag ttcgacgcaa tcctgctcgg cgcggtcggc acgcccgagg
     1981 tcaagccggg tgtgatcgag cgcggcctgt tgctgaagat gcgcttcgac ctcgacctgt
     2041 acgtcaacca gcggccattc gtcggtgtcg gtcccggtca caccgtcggc cacgagttcg
     2101 tcgtcatccg cgagaacacc gaaggccctt acgtcggcga gggcggcgtc ctgcgcaagg
     2161 gcacgccgca cgaggtggcc acccagggca gtgtcaacac ccgcttcggc gtcgagcggg
     2221 tgattcgcta tgcgttcgaa ctggccgcga cacgcgagcg caagcacgtc acgttggtgc
     2281 acaagaccaa cgtgttgtcg ttcgccggcg atctgtggca gcgcaccttc gacgacgtcg
     2341 ctgccgagta cccgggcgtc ggcaccgcct nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncgtca ccgacaacct gttcggcgac
     2461 atcctcaccg acctcggcgg tgtggtgtcg ggcggcatcg gtctggccgc gtcggcgaac
     2521 ctcaacccgt ctcgcaccgg cccgtcgatg ttcgagccgg tgcacggctc ggcccccgac
     2581 atcgtcggta ctggcaaggc caaccccgtc gcggcgatcc tgtccgcggc actgatgctc
     2641 gacttcctcg gcgagaccgc cgcggccgcg aaagtgcgtg ctgcctgcga ggatccgggc
     2701 actggcacca ccaccgagat cggtgaccgc atcgccgcgc gcgtcgccgg ctgacatatc
     2761 ctcggcgtca tgccgatcca aacgacaccg aagatctgga tgaacggtga gctcgtcgac
     2821 tgggacaagg cccaggtcca cgtcctcacc cacacgctgc actacggcac gggtgtgttc
     2881 gagggcatcc gtgcctatgc gaccgccgac ggcccggcgg tgttccgcct gaccgaccac
     2941 atcaagcggc tcttcaacag cgccaagatc gtcgggatgg agatccccta cacggtcgac
     3001 gagatcgtcg aggccaccaa gcagacggtg gcatcgaccg ggctcgacgc gtgctacgtg
     3061 cgccccctgg cctactacgg ctacggcgag atgggcctca acacgttgcc gtgcaccacc
     3121 gatgtcgcca tcgcctgttg gccgtgggcg ccgttcctcg gccatgaagc ggtgcggatg
     3181 aagaccannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnaaggcc gggtacgacg
     3301 aggccatcat gctggccccc aatggcctgg tggccgagtg caccggcgag aacatcttcg
     3361 ccgtgcgcaa cggcatcatc atcaccccgc cgctgtcggc cggggcgctg gagggcatca
     3421 cccagagcag cgtcatcacc atcgcccgcg atctcggcat ggacatccgc gtcgacaacc
     3481 tggcccgcag cgacctgtac atcgccgaag aggtgttcgt gtgcggcacc gcggccgaga
     3541 tcagcagtgt gcgttcgatc gacgaccgcg agatcccggc caacggcccg atcaccaatg
     3601 cgatctccga ggtctacctc aaggccgtcc gcggcgagga cgaccgctac aagcactggt
     3661 gcgagctcgc cgtctgaacc ccggtcgatc cgtcggcggc cgggctcggc ccccgacacg
     3721 ggtcgaaaac ggcgaccgcc ctcggggcgc tgcgggcggt ggggcctgcg cgccgccgag
     3781 ggcggtacag atgggagatc caccattgat cgatcgtccg gcgttaacct ttggctcgtt
     3841 caacacctca cccgtcagag caagctctgg cgggtgagtc cgttttcggc ctccccctgg
     3901 cggtatcccg gcgaaccggt ctgccacccc tgagttggtc gaagtgcgga tctcccgccg
     3961 gctcgtcgtt ccctctgtcc ccgtcgggcg ctgtgagcgg cttgcgctgc gcacgttgcc
     4021 cggtgagcca ggtggccggt acgaaccgga gtgagcacat cttgtacccc ctgaaagtcc
     4081 tggtcaagcg gtatttttca agtcacaggg ttatccacca acaaacccgt caatccccag
     4141 aagttttccg ctcgtccacc ccgtgtacac aacttgcaca caagcgaggt cacctcacgc
     4201 gagtggggcc aagcgagcga gggtggggag ggtgagattg gcgagggtgg gatggagggt
     4261 gaggccgggg gctgggtcga tcgccttgac gttggggacg ccgagcacgg tgcagccagc
     4321 ggccaacgcc gaggtggcgc cggtcggtga gtcctcgatc gcgaggcagt cgtgcgggtc
     4381 cacacccgca gcggccgcgc ccaggcggta gggcgtgggg tggggcttgc cttgcccggc
     4441 gggcacgtcg tctccggcga tcaccgcggt gaacacgccc ggatcgagag cggccacgat
     4501 cggacgggtg aggcgcttcc acgacatggt gaccaacacg ttggggatgc ccgcggcgtg
     4561 gacctcagcc aggagctcac gggctcccgg ccgccacggg atcgcaccgt cgagttgggc
     4621 ctcgacccgc tcgatcagcc agtcgacgat cgcccgtggt gtcaagtcga catgggcgtg
     4681 ggcgcggatg tactcggccg cgttgatcag gtcgaacccg acgatcgcct gggcgtggtg
     4741 gtggttccac tcactgccga actgggccac gaggtcgtac tcggcctgca tccagtacgg
     4801 ctcggtgtcg acgatggtgc cgtccaagtc ccacaacacc gcagccggcc acgtcggcga
     4861 cgcggcctgg gtgtcagggt cggtatcggc ggaagcggaa gcgggggcga aagtgggagc
     4921 ggcgctggcg gtgtgatcgg gggagggacg gttcacggcc gggcacggta tcgccgcccg
     4981 cgcgccggat gtggttgacg atcgtcccgc ggaggggcag aatggctccg atgtgtcttg
     5041 accgcgccac cctgcgccct ctcgcgatcg ccattatttg ttagcgcacg tctgccagac
     5101 gtgcgccttt tgccgcccac cggggcggct tttttgttgc ccgaaacccc cggagatccg
     5161 ccatgagcac tgaaccgaac cccgaatccc acagccgttc ccttcccaca cgcgtcgagg
     5221 tgttcgacac caccttgcgc gacgggctcc aggtcgaggg cgtctccgcg accgtcgaag
     5281 acaagctgcg catcgcagaa cagctcgact tcctcggcgt gcacttcatc gaaggcggct
     5341 ggccgggcgc caacccgaag gacatcgagt tcttcaaccg tgcggccaag gagctgcgct
     5401 tcaccaccag ccgcatggtg gccttcggtt cgacccgccg ccccaagggc aaggtcgacg
     5461 acgacgcgac gctgcgcaac ctgatcgaag ccggcaccga cgtggtgtgc atcgtggcca
     5521 agagctggga ctaccacgtg ctcaacgcgt tgcagaccac gctcgacgag ggcgaggcga
     5581 tgatcagcga ctcggtggcg ttcctggccg cccacggcaa gcaggtgctg gtcgacatgg
     5641 agcacttctt cgacggcttc aagcgcaacc ccgagttcgc gctgcgcgct gtggaggcgg
     5701 cgatcatcaa gggcgcgagc cacgtcgtac tgtgcgacac caacggagga tcgctgcccc
     5761 acgaggtgtt gcgcatcgtc ggtgacgtca aggcccacat cggcgacgac gcggtcatcg
     5821 gcatccactg ccacgacgac acggggtgtg cggtcgccaa cagcatggcc gcggtgctgg
     5881 ctggcggcgg ccatgtgcag ggcacgctga acggattggg cgagcggacc ggcaactgca
     5941 acctcaccac ggtcatcccc aacctgcagc tcaagcttgg catcgagtgc ctgcccgagg
     6001 gacgcctgga gcggctgacc tcggtgagca accacatcgc cgaggtcctc aaccgtccgc
     6061 tcaacccgca ggcgccgtac gtcggtgcct cggcgtttgc ccacaaggct ggcctgcacg
     6121 tgtcggccat cgcccgcgcc aaggacgcct acgagcacgt cgaccccgat ctcgtcggca
     6181 acagcacccg gttcgtcgtc agcgagatgg ctggtcgggc gacgctgaac atcaaggccg
     6241 acgagttggg catctccctc gacgggccag cgctgaacca ggtgatcgac gacctgaagc
     6301 ggctcga
//
LOCUS       DAORNF010000092         6289 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_9202_length_6289_cov_1.828361, whole genome shotgun sequence.
ACCESSION   DAORNF010000092 DAORNF010000000
VERSION     DAORNF010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 6289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6289
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9202_length_6289_cov_1.828361"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            110..>2299
                     /locus_tag="PLV68_05155"
     CDS             110..>2299
                     /locus_tag="PLV68_05155"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HRE00663.1"
                     /translation="MTRRAATRRRATRGALVALAVVGLGAPVVGGQVGAAPDPDAPDA
                     VNPAITAPFSLVAAGGGVDPAVSGNGQYVVYSAAPGTDDGRTSSVWMVNRQDGTATEL
                     TLPVDGVRVGNSVHPTISADGCTIAVVTEIPYDLFRDDDRGARWDVYAGRNQACGGNA
                     NDWVLVSTATDDIGQAAARNDVASDQAPSVSSAGSAIAYVRPFVSRTGNDDPDRPAAA
                     IDLVDLAVPVDEPTRVTAVPGLPTDAADNGVDYVGHTAPRLSADGTTVVFASDANAEL
                     AVATWGTPDPVTRVAPTQIYAWNRLDLDPFTAVRMLSVGGGTPGNASSSDPSVSDDGR
                     VVAFVSRATNLVSADASAFCGTACASQVFVVDRDAERTSLVSHTMATAAALGAETAPI
                     FGNGDSYAPALSGDGISVAFATKATDLLPVQAPGSGAPSDGDVIVADLSTSQAVLRRG
                     FDSLTPAPAVHGHPQLSANGRVLVVDSAAAGDILGDPSIIGRTIVAARFQPALSIASL
                     DLGTGLVGRASQGWEVNIINLGPGAINPVITIDNPDFSIVGGSCMDGVRVKAGSSCSI
                     SLLLTPSVVGAITGNLIVAEEGFDPLLVVTPLRGAGGEPALSSSPAVGDYGTEVVGTV
                     SYQTQTFHVYSVAWQPVWVHRADIAGANPDDFRVVSSTCGDAQLDTDAGCDVEVSFRP
                     TAAGRRTATLSFSTVAGQYTSVLLAGVGVYQPELIAAGTAEPGFFLPL"
     assembly_gap    2300..2318
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2319..2516
                     /locus_tag="PLV68_05160"
     CDS             <2319..2516
                     /locus_tag="PLV68_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00664.1"
                     /translation="ANSEVLLSWSDGQGWTELVTADDTGFFLANLLVSRNQRPGEATL
                     VARTADGVTASTTVLIERSRR"
     gene            complement(2585..3883)
                     /locus_tag="PLV68_05165"
     CDS             complement(2585..3883)
                     /locus_tag="PLV68_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00665.1"
                     /translation="MVAQADFSTSAIQVAPGDTAAVDLTIINLGNRTETFTAVPTGLL
                     AGWVRLDPPTVTLFGGSSQRVSVTLSPPLLATTPAGDAPLTVRVIPQSEPDEVVLAET
                     TASIDAFHDRRLNVLQPARLPKGGEAIPFVSDFFDFSIYEFLVENHGNSQASCRLHLI
                     DDSRRLDGDFDPPAVGVEPGGRSLVRLKLKAVRPQWWRGSRTLPFTIRADQPGYPSTS
                     AEATMVQTPVLPERLGRRTLALVVVAGAGVAGWFAGLKPLVERTARDATAAATPSTVA
                     NTGPAVTVTSTAPNTGDNTNTTSTDAPSSTVATVAPIDQVSVNQRLNMKSQKGQLNSA
                     KVTVPPGKEWRISDISLLNSNDDRGKVTVYRNDDELVSWRIERALPNIEAWKLDSPLV
                     LNAGDVIRAELLCDSFGTPLLGQCEDAVYVNGIEIDASSR"
     assembly_gap    4001..4113
                     /estimated_length=113
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4114..4963
                     /gene="hemB"
                     /locus_tag="PLV68_05170"
     CDS             <4114..4963
                     /gene="hemB"
                     /locus_tag="PLV68_05170"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HRE00666.1"
                     /translation="VSQLTLADLVTEVGDLIHLGVRAVILFGVPAVKDPIGSGAFDPD
                     GIVQVALRELRATYGDRIVLMADLCVDEYTDHGHCGVLTANGEVDDATLAIYAKAAVA
                     QADAGAHVVAPSGMMDGQVGAIRAALDAAGHTGVSILAYSAKYASGLYGPFRDAVDVQ
                     IAGGGHRKGYQQDWRNAREAMIELDADIAQGADMVMIKPALAYLDVIAAARERTNVPV
                     AAYQVSGEYAMVKAVAANGWIDHDAVALEQLTAIKRAGADMILTYFTRWFAEHRPTVQ
                     HQGVQA"
     gene            4960..>6289
                     /locus_tag="PLV68_05175"
     CDS             4960..>6289
                     /locus_tag="PLV68_05175"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HRE00667.1"
                     /translation="MIAVCDPVRCPPAGCVATLCTGAGIASNRELFERSREAIPGGVN
                     SSIRAFKSVGGSPYLVARAEGPYVFDVEGTRYIDLVQSYGAVILGHAHPAITAAVQAA
                     ANGGTSYGAPTPREMKLAEAIGERVPSCERVRLMNSGTEATSTAIRLARGVTGRDLIM
                     TFSGNFHGATDALLVAGGSGIATLGLPGTAGVPDATVANTMIGPYNVVPTLSADVAAV
                     IVEPVAANMGVVAPVPGFLEGLRAECDRVGALLIFDEVITGFRLGLGGAQAKYDVTPD
                     LTCFGKVIGGGLPIGAVGGRADVMDGLTPVGSVFHAGTLAGNPIATAAGLAALDRLDG
                     DVYMELLARARRVSGVLRDACDAAGLVAQFPVVGSLVGMYFGDGPLPVNFDQAKTTDE
                     AAYAAFFHAALAEGLALAPGAYEAVFVGLGHDDAVIDEIGERASRAAHRAA"
BASE COUNT          907 a         2126 c         2127 g          997 t
ORIGIN      
        1 cgagttggac ggcgccgact aacattccgg aggtctcggc atcaccgagc acaacgaggg
       61 caacgggctc aacagcctca cagggttagg gcaggacgcg gcgagcagca tgacacgacg
      121 agcagcaaca cgacgtcggg ccacccgcgg cgcgctcgtg gcactggccg tggtcggtct
      181 cggtgccccc gtcgttggcg gccaggtcgg agccgccccc gatcccgacg cgcccgacgc
      241 cgtcaatccg gcgatcactg ccccgttcag cctggtcgcc gctggtggcg gcgtcgatcc
      301 agcggtctcg ggcaatggcc agtacgtcgt ctacagcgcc gcgccgggta ccgacgacgg
      361 tcgtacctcc agtgtgtgga tggtgaaccg ccaagacgga acggccaccg agctgaccct
      421 gccggtcgat ggtgtgcgcg tcggcaacag tgtccacccg accatcagcg ccgacggctg
      481 caccatcgcc gtcgtgaccg agatccccta cgacctgttc cgtgacgacg accgtggcgc
      541 ccgctgggac gtctacgccg ggcgcaacca agcctgtggt ggcaatgcca acgactgggt
      601 gttggtctcg accgccaccg acgacatcgg gcaggccgcg gcccgcaacg acgtcgcgtc
      661 cgaccaggca ccgagcgtgt cgtcggctgg ctcggccatc gcctacgtgc gtcccttcgt
      721 gtcacgcacg ggcaacgacg accccgatcg cccggctgcg gcgatcgatc tggtcgactt
      781 ggcggtaccg gtcgacgaac cgacgcgggt caccgccgtg cccggcttgc cgaccgatgc
      841 tgccgacaac ggcgtcgact acgtgggcca caccgcaccg cgcctgtcgg ccgacggcac
      901 cacggtggtg ttcgcgtccg acgccaacgc cgagttggcc gtcgccacct ggggcacccc
      961 agacccggtg actcgcgtcg cgccgaccca gatctacgcc tggaaccgcc tcgatctcga
     1021 tccgttcacc gcggtgcgga tgttgtcggt cggtggcgga acgcctggca atgcttcgtc
     1081 cagcgaccca tcggtcagcg acgatggtcg cgtggtggcg ttcgtgtcgc gggcgaccaa
     1141 cctggtcagc gccgacgcct ctgccttctg tggaacggcc tgtgccagcc aggtcttcgt
     1201 ggtcgatcgc gacgccgagc gcacctcgtt ggtgagccac acgatggcga ccgctgccgc
     1261 gctcggcgcc gagacggccc cgatcttcgg taacggcgac tcgtacgctc ccgcgctgtc
     1321 gggcgatggc atctcggtcg ccttcgccac caaggccacc gacctgttgc cggtgcaggc
     1381 gcccggcagt ggtgccccga gcgatggcga cgtcatcgtc gccgatctgt ccacgtcgca
     1441 ggccgtgttg cggcgcggct tcgacagcct gacgcccgcg cccgcggtcc acggtcatcc
     1501 gcaactgtcg gccaacggcc gcgtgctggt ggtcgattcg gccgcggcgg gcgacatcct
     1561 cggcgacccg tcgatcatcg ggcggaccat cgtcgcagcc cgcttccagc ccgcgctctc
     1621 gatcgcttcg ctcgacctcg gcaccggcct ggttgggcgt gcctcacaag ggtgggaggt
     1681 caacatcatc aacctcggcc ccggcgcgat caaccccgtg atcaccatcg acaaccccga
     1741 cttctccatc gtcggtggtt cgtgcatgga cggggtgcgg gtcaaggctg ggtcgagttg
     1801 ctcgatcagc ctgctgctga ccccctcggt cgtcggcgcg atcacgggca atctgatcgt
     1861 ggccgaagag gggttcgatc cgctgctggt cgtcaccccg ctgcgtggtg ccggtggcga
     1921 accggcgttg tcgtccagcc ccgcggtcgg cgactacggc accgaagtgg tcggcacggt
     1981 cagctaccag acgcagacct tccacgtgta cagcgtggcg tggcagcccg tgtgggtgca
     2041 ccgggccgac atcgccgggg ccaatcccga cgacttccgg gtggtgtcgt ccacctgtgg
     2101 cgacgcgcag ctcgacaccg acgcgggctg cgatgtcgaa gtctcgttcc gccccaccgc
     2161 ggcggggcga cgcacggcaa ccctcagctt ttcgaccgtg gccgggcagt acaccagcgt
     2221 gctgctcgcg ggcgtgggcg tctaccagcc cgaactgatc gcggctggga ctgccgagcc
     2281 aggcttcttc ctccccctcn nnnnnnnnnn nnnnnnnngc caacagtgag gtgttgttgt
     2341 cgtggagtga tggccagggc tggaccgaac tcgtcactgc cgacgacacc ggcttcttcc
     2401 tggccaacct gttggtgtca cgcaaccagc ggcccggtga agccaccttg gtggcccgta
     2461 ccgccgatgg tgtcacggcc agcaccacgg tgctgatcga gcggtcgcga cgctgatccc
     2521 ggcgccttcc gtctcacgta cacagacata tgccgcagca accatatgcc gcagcaacgc
     2581 ggtgtcagcg cgacgatgcg tcgatctcga tgccgttgac gtagaccgca tcctcgcact
     2641 gtccgagcaa gggcgtgccg aacgagtcgc acaacagttc agcccggatc acgtcaccgg
     2701 cgttgagtac cagcgggcta tcgagtttcc aggcctcgat gttggggagc gctcgttcga
     2761 tgcgccagct caccagttcg tcgtcgttgc ggtagacggt gaccttgcct cgatcgtcgt
     2821 tgctgttcaa caggctgatg tcgctgatgc gccactcctt gccgggtggc accgtgacct
     2881 tggcagagtt gagttggccc ttctgggact tcatgttcag gcgctggttg accgacacct
     2941 gatcgatcgg ggccacggtt gcgacggtgc tcgacggggc gtcggtggac gtggtgttgg
     3001 tgttgtcgcc cgtgttgggt gcggtggacg tcaccgtgac ggctggtccg gtgttggcga
     3061 cggtggaggg ggtggctgcc gcggtggcgt ctcgggcagt gcgctcgacc aacggcttca
     3121 gcccggcgaa ccagccggcg acacctgcac cagccacgac gaccagcgcc agcgtgcgcc
     3181 gaccgagccg ctcgggcagc accggggtct gcaccatggt cgcttcggcg ctggtcgacg
     3241 gatagcccgg ctgatcggcc cggatcgtga acggcagcgt gcggctgcca cgccaccact
     3301 gcgggcgcac agccttcagc ttcaggcgca ccaggctgcg cccgcccggc tcgacaccca
     3361 cggcaggggg atcgaagtcg ccgtcgagtc ggcgtgaatc gtcgatgaga tgcaggcggc
     3421 agctggcctg ggaattgccg tggttctcga ccaggaactc gtagatcgag aaatcgaaga
     3481 agtcggaaac gaaggggatc gcctcgccgc ccttcggcaa gcgcgccggc tgcagcacgt
     3541 tgaggcggcg atcgtggaag gcgtcgatcg aggcggtcgt ttcggccaga acgacctcgt
     3601 ccggctcgct ttggggaatg acgcgcacgg tcagcggtgc gtcgcccgcg ggagtcgtgg
     3661 ccaagagcgg tggcgacagc gtgaccgaca cgcgctggct ggagcccccg aacagcgtga
     3721 ccgtcggcgg atcgaggcgc acccatccgg cgagcaggcc ggtcggcacg gcggtgaagg
     3781 tctcggtgcg gttgccgagg ttgatgatgg tcagatcgac cgccgcggtg tcgccggggg
     3841 ccacctgaat tgccgatgtc gagaagtcag cttgggccac catcgtcggc cgacgatagc
     3901 ggtcgggcac ccgaagtcct gggcacgccg caccactacg ctggcgacgt gactgcccac
     3961 tttcctgtcg cgcgtccgcg tcggctgcga tccaccgatg nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncgtcagc cagttgaccc tcgccgatct
     4141 cgtcaccgag gtcggcgact tgatccacct cggtgtgcgg gcggtgatcc tgttcggggt
     4201 ccctgcggtg aaggacccga tcgggtcggg cgccttcgac cctgacggca tcgtgcaggt
     4261 cgcgttgcgc gagctgcgtg cgacctatgg cgaccggatc gtgctgatgg ccgacctgtg
     4321 cgtcgacgag tacaccgacc acgggcactg cggtgtgctc accgcgaacg gcgaggtcga
     4381 cgacgcaaca ctcgcgatct acgccaaggc cgccgtcgcc caggccgacg ccggggcgca
     4441 cgtggtcgca cccagcggga tgatggacgg ccaggtcggt gccatccgcg ccgcgctcga
     4501 tgctgccggg cacaccgggg tgtcgatcct ggcctactcg gccaagtacg ccagcgggct
     4561 gtacggcccg ttccgcgacg cggtcgacgt gcagatcgcc gggggcgggc atcgcaaggg
     4621 ctatcagcag gattggcgca atgcccgcga ggcgatgatc gagctcgacg ccgacatcgc
     4681 ccaaggcgcc gacatggtca tgatcaagcc cgcgctggcg tacctcgacg tgatcgccgc
     4741 cgcgcgcgag cgcaccaacg tgccggtggc cgcctaccag gtcagtggcg agtacgcgat
     4801 ggtcaaggcc gtggcggcca acggctggat cgatcacgac gcggtggcgc tcgaacagct
     4861 caccgccatc aaacgagcgg gagccgacat gatcctcacc tacttcactc gctggttcgc
     4921 cgagcatcgc ccgaccgtcc aacaccaggg ggtgcaggca tgatcgccgt ctgcgacccc
     4981 gtccgctgcc caccggctgg gtgtgtggcg acgctgtgca ccggggcggg catcgcgtcc
     5041 aaccgcgagc tcttcgagcg ctcgcgcgag gcgatccccg gcggggtcaa ctcctcgatc
     5101 cgggccttca agtcggtcgg tggctcgccg tatctggtcg cccgtgccga gggcccgtac
     5161 gtgttcgacg tcgaaggcac gcgctacatc gacctggtgc agagctacgg cgcggtcatc
     5221 ctcggccacg cccatcccgc catcaccgcc gcggtgcagg ccgctgcgaa cggcggcacc
     5281 tcgtacggcg caccgacgcc gcgggagatg aagctggccg aagcgatcgg cgagcgcgtc
     5341 cccagttgcg agcgggtgcg gctgatgaac agcggcaccg aggcgaccag cacggcgatc
     5401 cgcctcgctc gtggcgtcac cgggcgcgat ctgatcatga ccttcagtgg caacttccac
     5461 ggcgccaccg acgcgctgtt ggtggccggc ggcagcggca tcgccaccct cggcctgccc
     5521 ggcacagccg gcgtccccga tgccaccgtg gccaacacca tgatcggccc gtacaacgtg
     5581 gtgcccacgc tgagtgccga cgtcgcggct gtcatcgtcg agccggtggc cgccaacatg
     5641 ggagtcgtcg cgccggtccc cggctttctc gaaggcctgc gcgcggagtg cgatcgggtc
     5701 ggcgcgctgc tgatcttcga cgaggtcatc accggcttcc gtctgggtct cgggggcgca
     5761 caggccaagt acgacgtcac ccccgacctc acctgcttcg gcaaggtcat cggcggtgga
     5821 ctgccgatcg gcgcggtcgg cggccgggct gacgtgatgg acgggctgac cccggtcggg
     5881 tcggtgttcc atgccggcac cctggcgggc aatcccatcg ccaccgcggc cgggttggct
     5941 gcgctcgacc ggttggacgg cgacgtctac atggaactgc tggcccgggc ccggcgggtg
     6001 tcgggcgtgc tgcgcgacgc ctgcgatgcg gccgggctgg tcgcccagtt cccggtcgtc
     6061 gggtcactcg tcgggatgta tttcggcgac ggtccgttgc cggtcaactt cgatcaggcc
     6121 aagaccaccg acgaagccgc gtacgccgcg ttcttccacg ccgcgttggc cgaagggttg
     6181 gccctcgcgc ccggcgcgta cgaggcggtg ttcgtcgggc tcggccatga cgatgcggtg
     6241 atcgacgaga tcggcgagcg ggcgtcgcgg gcggctcacc gtgccgccg
//
LOCUS       DAORNF010000093         6273 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_9236_length_6273_cov_1.849308, whole genome shotgun sequence.
ACCESSION   DAORNF010000093 DAORNF010000000
VERSION     DAORNF010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 6273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6273
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9236_length_6273_cov_1.849308"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>273)
                     /locus_tag="PLV68_05180"
     CDS             complement(<1..>273)
                     /locus_tag="PLV68_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00668.1"
                     /translation="EPIADCTDVAIEVAVDGQTATAVCDSVFPADTFADVSAVYSGTT
                     RWNSSADSTDVDGLGLVPTATELTTSIDGGEPGDAIRLAATVTAPRP"
     assembly_gap    274..283
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<284..2062)
                     /locus_tag="PLV68_05185"
     CDS             complement(<284..2062)
                     /locus_tag="PLV68_05185"
                     /inference="COORDINATES: protein motif:HMM:NF013959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrin alpha"
                     /protein_id="HRE00669.1"
                     /translation="MAGNPRCDAGSNVAGDTARQPGRPGSAAPDPTEVTPDGTPSAIL
                     KYVGDTSSATGAALSNSTCDVNGDGYNDAVVGAWFWDKAPTNNIGAAYVLFGGPDVTG
                     GSLGTPGNVDAVRIDGPNVANAFVGFAVGCVGDVNGDGLDDFGISYYTDQKTYVLFGD
                     TNFTTLSLSSLGDRGFVVSAPVSGGNLGFSLAAVGDLNNDGLDDFAVAAVVADTQGRT
                     NNGRVWVIAGQDDIADVNVDNPVAGQVLFAIDGASSEERVGVMSVIGDVNGDGVDDIL
                     LGSYTATPWGTSYAAPGAAYVVWGGQSVDVDLANLGTKGFAIYGSSRQRDRLGISVSG
                     LGDINGDGKADLLIGADGVTNATTGNREGGAAVVFGSAATDTVYTDPTSDTLGVYTCS
                     GSQTLGVCADPAEATARGYWISGDFTNDSAGYSVAGLGDINGDGINDMVIGAYGYDPI
                     NPANPPATMSGAGAAYVVFGKTDTATVKLADVTTALGSLGYRVEGLVAGDRMGRQVAP
                     AGDFDGNGYPDVVIGSDFAPRGGSQNGEITILLLGPLGTTAAVTSDVAEGYVGDPITL
                     TATVTTATQLTPATMTTPNLDGGTVTF"
     gene            complement(2456..3556)
                     /locus_tag="PLV68_05190"
     CDS             complement(2456..3556)
                     /locus_tag="PLV68_05190"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRE00670.1"
                     /translation="MKPRKQEQERATDQITEIAPNVLRSELPIELPGLGHVNMYLLED
                     ERGVAVVDPGLPEKASYAAVEARLKAAGYPIERVHTIVITHSHHDHYGGAAWLQERSG
                     GDAKIVTHRDFQMMWEPVDVPDVDVEDFAELAGEATLDGQDQELQSKRRVPWAPPVWG
                     GPGMDLPLKRKLMFYTARYLPMMRRRLPRPTNRLVEAETIRLARRDWIAVHTPGHTND
                     HLCLFDPTEGCMICGDHVLPTITPHIGGMGGRELDPLAAFFPSLDKMVSFGDQVTVAL
                     PAHGRPFNDLAGRSREIKEHHLGRLELLRTTSATLGRPASVMEMSTYLFSPRAQGVMA
                     DSETYAHLEYLRLRGEMVRNSTDGVYEYVLPG"
     gene            complement(3579..>3905)
                     /gene="orn"
                     /locus_tag="PLV68_05195"
     CDS             complement(3579..>3905)
                     /gene="orn"
                     /locus_tag="PLV68_05195"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-exoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HRE00671.1"
                     /translation="TLAFIKQHVPVERTVPLCGNSIGMDRRFLNAYLPEIENWLHYRS
                     VDVSSIKELVRRWYPQVKSDLPHKNGNHRALDDIRSSINELIFYRDRAFVPIPGFTPS
                     SETTVR"
     assembly_gap    3906..3915
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3916..4160)
                     /locus_tag="PLV68_05200"
     CDS             complement(<3916..4160)
                     /locus_tag="PLV68_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HRE00672.1"
                     /translation="MLVWMDLEMTGLDASKDVIVEIATIVTDDQLRIVAEGPDLVIHQ
                     PPQALELMVPFVRDMHTRSGLLAAIEAVHHRYPNPCP"
     gene            4275..4862
                     /locus_tag="PLV68_05205"
     CDS             4275..4862
                     /locus_tag="PLV68_05205"
                     /inference="COORDINATES: protein motif:HMM:NF016102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HRE00673.1"
                     /translation="MPHTMIEAMGMRTENDCLGSVDGHMVLFVVFVLAPVIELYTLIK
                     VGSAIGGWNTIGLLIVIAMIGSWLIKREGFKAWTRFAASMQSGQPPARPLADGVCVLL
                     AGLLLMVPGFVSDVLALLLLFPPTRAIARAWLLRRTKLGRMGGLGRNTVITATYGGRM
                     PDAPHDLRDGVITTTGTETATETARPETPRGEIER"
     gene            4859..5707
                     /locus_tag="PLV68_05210"
     CDS             4859..5707
                     /locus_tag="PLV68_05210"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRE00674.1"
                     /translation="MSFEFAAPLSDPDFDPTTVPVRPAATVMLVRDSDDTGDLEVFML
                     RRTAQAAFGGGMYVFPGGRLDAVDSSAEMESVCDGLDDTRASSLLGVERDGLAFWVAA
                     IRECFEEAGVLLARSSSTGDVLRFDDPEVAARFAAERGRIYDSSTNLVNLCAAEGLRL
                     ATDTIHYTAHWITPVGERRRFDTRFFVARAPHAQEPLHDDGETVASLWVRPGDALTRQ
                     AAGDLLMMPPTMSSLRFLADFASADAACTAAATLGPPPPIQPRLVTTADGRIRVLMPG
                     DDGYDA"
     gene            complement(5979..>6273)
                     /locus_tag="PLV68_05215"
     CDS             complement(5979..>6273)
                     /locus_tag="PLV68_05215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00675.1"
                     /translation="GLFGVHHEVDLALAEEIEAPLLGQVPLEPSLAAGGDAGVPVAMV
                     APLTAAGQVFHDIAQRIVDDIAPPVNMAGCSARMLAAVSAAFDKVAPSAPVSA"
BASE COUNT          954 a         2052 c         2175 g         1072 t
ORIGIN      
        1 ggccgcggcg cggtgaccgt tgcggcgagg cggatggcat cgcccggctc accgccgtcg
       61 atcgaggtgg tcagttcggt ggcggtcggc accaggccga gaccgtcgac gtcggtgctg
      121 tcggccgaac tgttccagcg ggtggtgccg gagtacaccg ccgagacgtc ggcgaaggtg
      181 tccgccggga acaccgagtc gcacactgcc gtcgcggttt gaccgtcgac ggcaacctcg
      241 atcgcgacgt cggtgcagtc cgcgatcggc tccnnnnnnn nnngaacgtc accgtgccac
      301 cgtcgaggtt gggcgtggtc atcgtcgccg gggtgagctg cgtcgcggtc gtgaccgtgg
      361 cggtcagcgt gatcgggtca ccgacgtagc cctcggcaac gtcggaggtg accgcggcgg
      421 tcgtgccgag cgggccgagc aacaggatgg tgatctcacc gttctgcgag ccgccgcgag
      481 gtgcgaagtc ggagccgatc accacgtcgg gatagccgtt gccgtcgaag tcgccagcag
      541 gggcgacctg acggcccatc cggtcgccgg ccacgagccc ttcgacccgg tagcccagtg
      601 aaccgagcgc ggtggtgacg tcggccagct tcaccgtcgc ggtgtcggtc ttgccgaaca
      661 ccacataggc cgcgcctgcg ccgctcatcg tcgccggcgg gttggcgggg ttgatcgggt
      721 cgtatccgta ggcgccgatg accatgtcgt tgatgccgtc gccgttgata tcgcccaacc
      781 ccgccacgga gtacccggcg ctgtcgttgg tgaagtcgcc gctgatccag tatccgcgtg
      841 cggtcgcttc ggccgggtcg gcgcacacgc cgagggtctg gctgcccgag caggtgtaga
      901 cgccgagcgt gtccgacgtc gggtcggtgt acaccgtgtc ggtcgcggca ctaccgaaca
      961 ccaccgctgc tccgccttcg cggttgccgg tggtggcgtt ggtgacaccg tcggcaccga
     1021 tcaacaggtc ggccttgccg tcaccgttga tgtcgcccag tcccgacacg gagataccga
     1081 gtcggtcgcg ctggcgggac gatccataga tcgcgaaccc cttggtgccc agattggcca
     1141 ggtcgacatc gaccgattgg ccaccccaca ccacgtaggc cgcgccgggg gcggcatagc
     1201 tcgtccccca cggcgtcgcg gtgtagctgc cgagcaggat gtcgtcgacg ccgtcgccgt
     1261 tgacatcacc gatcaccgac atcacgccga cgcgctcttc ggacgacgcg ccgtcgatgg
     1321 cgaacagcac ttggcccgcg acggggttgt cgacgttgac gtcggcaatg tcgtcttgac
     1381 cggcgatcac ccatacccgg ccgttgttgg tgcggccctg cgtgtcagca acgacggccg
     1441 cgacggcgaa gtcgtccagt ccgtcgttgt tcaagtcgcc caccgcggcc agggagaagc
     1501 cgaggttgcc gccgctcacc ggggcagaca ccacgaagcc gcgatcgccc agtgacgaca
     1561 ggctcaacgt cgtgaagttg gtgtcaccga agagcacgta cgtcttctgg tcggtgtagt
     1621 agctgatccc gaagtcgtcg aggccgtcgc cgttgacgtc gccgacgcaa ccgaccgcga
     1681 acccgacgaa cgcgttggcc acgttcgggc cgtcgatgcg gaccgcgtcg acgttgcctg
     1741 gcgtgccgag gctgccaccc gtcacgtccg gcccaccgaa caacacgtag gccgcgccga
     1801 tgttgttggt cggggccttg tcccagaacc acgccccgac cacggcgtcg ttgtagccgt
     1861 cgccgttgac gtcgcaggtc gagttgctca gcgccgcgcc ggtggccgac gaggtatcgc
     1921 cgacatactt cagaatcgcg ctcggcgttc cgtctggcgt gacctcggtg gggtcgggcg
     1981 cggcgctgcc ggggcgaccg ggctggcgag cggtatcgcc agcgacgttg ctgccagcgt
     2041 cgcagcgagg gttgcccgcc accgcgtctg ggtcgtgatg ctcatcaatt gctcctcgtc
     2101 ggctcgtacg ccaggtgatc ggagggcgat caccgaatgt cgcgtagccc tacctttata
     2161 gataaggcag accttataaa cgaggggaag ctagcggtcg cgatatgcct cggtcaaggt
     2221 ggtgtcggtc ctggcagcgc gggtcgatgc ggccagggtc gaggcggcac gggtcacggt
     2281 ggcgcgggtc aaggtggcac cgggtacgac gtcgtgggtt acgacggcac gcccagcgcg
     2341 catgcccacg gtgtcagcgc aagcccgcgg tgtcttggtg aggccatgcg aggccggggc
     2401 cgggcgcggc ccccgtcgcg gcggaccggg gcggtctgga gagtcggccg gggggtcagc
     2461 ccggaaggac gtactcgtac acgccgtcgg tgctgtttcg taccatctca ccgcgcaggc
     2521 gcaggtattc gaggtgggcg taggtctcgc tgtcggccat cacaccttgg gctcgtggcg
     2581 agaacaggta cgtcgacatc tccatcaccg aggccggtcg ccccagggtt gccgacgtgg
     2641 tgcgcagcag ttcgagccga ccgaggtgat gttccttgat ctcgcgcgag cgtccggcca
     2701 gatcgttgaa cgggcggccg tgggcgggca gcgcaacggt gacttgatca ccgaaggaca
     2761 ccatcttgtc gagcgagggg aaaaacgccg ccaacgggtc gagttcgcgc ccgcccatac
     2821 cgccgatgtg gggcgtgatc gtcggcagca cgtgatcgcc gcagatcatg catccttcgg
     2881 tcgggtcgaa caggcacagg tgatcgttgg tatggccggg ggtgtgcacc gcgatccagt
     2941 cgcggcgggc caagcgaatc gtctccgctt cgaccaggcg gttggtgggg cggggcaagc
     3001 ggcgacgcat catcgggaga tagcgcgcgg tgtagaacat cagcttgcgc ttgagcggaa
     3061 gatccatgcc aggaccgccc cacaccggcg gcgcccacgg cacgcggcgc ttgctctgca
     3121 gttcctgatc ctgtccgtcg agggtggcct cgccagccag ttcggcgaag tcctcgacgt
     3181 cgacgtcggg aacgtcgacg ggttcccaca tcatctgaaa gtcgcgatgg gtgacgatct
     3241 tggcgtcgcc gccggaacgc tcctgcagcc aggccgcgcc tccgtagtga tcgtggtggc
     3301 tgtgggtgat gacgatcgta tggacccgct cgatcgggta gccagcggcc ttgaggcgcg
     3361 cctcgacagc ggcgtacgag gccttctccg gcaggccggg atcgacgacg gcgacgcccc
     3421 gctcgtcttc gagcaggtac atgttgacgt gccccagacc ggggagctcg atcggcagtt
     3481 cagaccgcag cacgttcggg gcgatctcgg tgatctggtc ggtggcccgc tcttgctctt
     3541 gcttgcgtgg cttcaccggc tgatctgtca taggtacgtc accgtaccgt tgtctcgctg
     3601 gacggggtga aacccgggat cggcacgaac gctcgatcgc ggtagaagat cagctcgttg
     3661 atgctggagc gaatgtcgtc gagcgcgcgg tggttgccgt tcttgtgcgg caggtcggac
     3721 ttgacctgtg ggtaccaacg ccggacgagc tctttgatgc tcgacacgtc gacgctgcgg
     3781 tagtgcagcc agttctcgat ctcgggcagg taggcgttga ggaagcgccg gtccatgcca
     3841 atcgagttgc cacacaacgg cacggtccgc tcgaccggca cgtgctgctt gatgaacgcc
     3901 agtgtnnnnn nnnnncacgg gcacggattc ggatagcggt gatgcaccgc ttcgatcgcg
     3961 gcgagcaggc cgctgcgggt gtgcatgtca cgcacgaacg gcaccatcag ctcgagagct
     4021 tgcggtggtt ggtggatcac cagatcgggg ccttcggcga cgatgcggag ctggtcgtcg
     4081 gtcacgatgg tggcgatctc gacaatcacg tccttgctcg catcgagccc cgtcatctcc
     4141 agatccatcc acaccaacat gcagcccagg ctagtgatcg agccccgtca ccccgcggcc
     4201 ggcgacgcgt accgcacgtg tgttcacgtg acccctcgca gcggggtcga cctgacgcag
     4261 ccgccctggc cccgatgccg cacacgatga tcgaggcgat gggcatgcgc accgaaaacg
     4321 actgcctcgg tagcgtggat ggccacatgg tgttgttcgt cgtcttcgtg ctcgctcccg
     4381 tgatcgagct gtacacgttg atcaaggtcg gcagcgcgat cggcggctgg aacacgatcg
     4441 gactgctgat cgtgatcgcc atgatcggca gttggctgat caagcgcgaa gggttcaagg
     4501 cgtggacccg ctttgcggca tcgatgcaat ccggtcagcc cccggcccgg ccactcgctg
     4561 atggggtctg cgtgttgctc gccgggctgt tgctgatggt gcccggcttc gtcagtgacg
     4621 tgctcgcgtt gttgctgttg ttcccgccca cgcgggcgat cgcccgggcg tggttgttac
     4681 gccgcaccaa gctcgggcgg atgggtggtc tcggtcgcaa cacggtgatc accgcaacct
     4741 acggtggccg catgcccgac gcgccgcacg acctccgtga cggcgtcatc accacgactg
     4801 gcactgagac tgccaccgag actgcccggc cggaaacgcc gaggggagag atcgagcgat
     4861 gagtttcgag ttcgctgcgc cgctgtccga cccggacttc gatccgacca ccgtgccggt
     4921 tcgcccggcc gccaccgtga tgttggtgcg cgacagcgac gacacagggg acctcgaggt
     4981 gttcatgctg cgccgcaccg cccaggcggc cttcggtggc ggcatgtacg tgttccctgg
     5041 cggacggctc gacgcggtcg attcgtcggc cgagatggag agcgtctgcg acggcctcga
     5101 cgacactcgg gccagttcgt tgctcggggt ggaacgcgac gggctggcgt tctgggtcgc
     5161 ggcgatccgg gagtgcttcg aagaggctgg ggtgttgctc gcgcgctctt catcgaccgg
     5221 cgatgtgctg cgcttcgacg atcccgaggt cgcggcccgc tttgccgccg agcgtggccg
     5281 catctacgac agctcgacca acctggtcaa cctgtgcgcg gccgagggct tgcgcctggc
     5341 gaccgacacc atccactaca ccgcacactg gatcacgccg gtcggcgaac gacgccggtt
     5401 cgacacccgc ttcttcgtcg ctcgcgcgcc gcacgctcag gagccgctgc acgacgacgg
     5461 cgagaccgtc gccagtttgt gggtgcgccc cggcgacgcg ctcacccgcc aggccgcggg
     5521 cgatctgttg atgatgccgc ccaccatgtc gtcgctgcgc ttcctcgccg acttcgcctc
     5581 ggccgacgcg gcctgcacgg cggctgccac gctcggcccg ccacccccga ttcagccccg
     5641 actggtgacc acggccgacg gccggatccg cgtcctgatg cccggcgacg acggctacga
     5701 cgcctgaccg atccaaccgt gtcgctttga ccccttgtgg cggggtcaat ccgactcggc
     5761 acgcaacagc tcttgcgggt gtgttcgatt caccccgtca gccggggtca acctgacgca
     5821 gtgcgctcgc cgaggcgggc gcgcgcggcg cgggcaccgt gcagatcacg gagaaggagg
     5881 tcgccacgct gcccggtgcc cagttcgtct tggagtccgc ccacatggaa ggcaccttca
     5941 tcgtctgcaa ggtcaaacaa gtccgctagc tgcgggggtc acgccgacac gggtgctgac
     6001 ggggcgacct tgtcgaaggc ggcggagacg gcggccagca tgcgggccga gcagccggcc
     6061 atattgacgg gcggggcgat gtcgtcgacg atgcgctggg cgatgtcgtg gaacacctgg
     6121 cccgccgcgg tgagcggtgc caccatggcc accggcaccc cggcatcgcc accggccgcc
     6181 agcgacggtt ccagcggcac ctggccgagc aacggggctt cgatctcttc cgccagggcc
     6241 agatcaacct cgtggtgaac accgaacagg ccg
//
LOCUS       DAORNF010000094         6248 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_9292_length_6248_cov_1.736315, whole genome shotgun sequence.
ACCESSION   DAORNF010000094 DAORNF010000000
VERSION     DAORNF010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 6248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6248
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9292_length_6248_cov_1.736315"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    207..359
                     /estimated_length=153
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1379..1960
                     /locus_tag="PLV68_05220"
     CDS             1379..1960
                     /locus_tag="PLV68_05220"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HRE00676.1"
                     /translation="MTRFFTSDLHFGHANVIGYCGRPFHGVGQMNAAMVAAWNTVVGP
                     DDEVWVLGDLAMGKLDESLQCAAQLVGRKHLVIGNHDRPFRGHGAARYEAVGFELHHG
                     HVELDMPNGVTVVACHFPYTGDSHDVDRYDEHRPADEGRWLLHGHVHERWRQRGRMIN
                     VGVDAWGGYPVTDTHLATLIDAGEADLRPLVWE"
     assembly_gap    2229..2259
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2260..3289
                     /locus_tag="PLV68_05225"
     CDS             <2260..3289
                     /locus_tag="PLV68_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00677.1"
                     /translation="VTTAGNTGSGEFTPGDVTYRVVNLLAVPVDLYARTQGFVEAYPL
                     QIGLDSLTVSEFYAPPENGRFLVTQAGATDVTCVSSCTEFIGEFAPYPDDGPVHTVVV
                     YDVGDGPKTFDLWEQALPPSQGNANAMAPADASAGLAIVTAIALTNSDFGLRFGWAGV
                     DGCHEPVNLAGVLVGGNQTPAYVYGGNAAGVVLFPNDDRDCAGAPVGGTFNIYGGAGT
                     RTHVILYGEPENMSAVVLPMAGDETAAADAEANPVYGEAASGDPAVRDAVIAAMAEGL
                     AREIDLTPAQAACASEYVVDALGLDVVAPGGVVVDFDSLGDAEAEIAGDALVAAVVPC
                     GIDPSVLG"
     assembly_gap    3500..3543
                     /estimated_length=44
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3731..4522
                     /locus_tag="PLV68_05230"
     CDS             3731..4522
                     /locus_tag="PLV68_05230"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HRE00678.1"
                     /translation="MASSLAGAAPSGVALHGDIPAGVGMPSVDARSVAVYDATAGRWL
                     GGSNADAQIAVGSVMKLLTSYVVMQAGDLSKVVTVPAMDVNFAESSIGLYQGQKLARD
                     VLLRAMMIVSANDAARALAVDVGGSTDNFVAKMNAAATSLGLANTHAANPIGLDATGA
                     YSSARDMVALAELLMRDAAFSAAVARTSANMHGTTFPATNKLLGSYQGANGVKTGHTT
                     QAGYCIVASATRNGRTIYVALFGAASDAARTTSTTALFDWAFAQA"
     gene            4667..5827
                     /locus_tag="PLV68_05235"
     CDS             4667..5827
                     /locus_tag="PLV68_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HRE00679.1"
                     /translation="MKLGAFFFGGVQMSDPGPGAPDPMDRRYSNEEVWAATKSYIDSA
                     VVADELGYNSFWTTEHHFQYEGYEVIPNGVQISTWIAALTQQITLGTMFNVVPQWNPL
                     RLAEDFSTLLNLSGGRAVLGVGRGTVPREVLHLNDKGVSIGSLDNPDQAAADVLNREV
                     FEESMEIIRRALNNERFSFSGKHFQIPVAGIPDRGTTVQELTLVPRPLYPFEIWQAVT
                     SPPTLDYVPVVGHGAVFWNQHYAFIKRFWDTYGAKYEQAHGEAIAPSEKRMLVVSARI
                     EDTHEQAMASARAGHDEFWKFLGPYGWSRGYMGDDGKPAKPGLIPTMEESIENKTILV
                     GSPADVAAEVQFLKDLLGFEHLTIFPHLLGDSYAKANEQMERFITEVVPLVN"
     gene            5857..6225
                     /locus_tag="PLV68_05240"
     CDS             5857..6225
                     /locus_tag="PLV68_05240"
                     /inference="COORDINATES: protein motif:HMM:NF014687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HRE00680.1"
                     /translation="MGSEWDDDGDVEPDVRFLYANERTFLAYNRTSLALVTAGLAITQ
                     LLPPFDIPGGRRMIGLPLIAMGTVMALASLFQWKRNEAAMRRGESLPRSILPILVTIV
                     VTASAAVALILAIFSDSGAS"
BASE COUNT          976 a         1980 c         2072 g          992 t
ORIGIN      
        1 aacaacgcgg agcaccacag aaaattccca agaactaacg actcacgaca ctagtggtgg
       61 gccaacgaat catgtctgcg tccgctgatc acatgacgcg gctgagtcgg cggccctggc
      121 cgttgcatgt gcctccgacg tcggtcgaca cgacgaacga gcaacgtcga gcgaacaccc
      181 ggacagcacc ctgcccgggt atcgccnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc
      361 ggcggccctg gccgttgcat gagcctccga cgtcggtcga cacgacgaac gagccaacgt
      421 tgagcgacac ccggacagca cgctgcccgg gcatcgccgc gtgcagccag ccctgctcgg
      481 tcgcgcctcg ggtacggata cgggcaccag ggtgagatga accgagttcg cccacctggt
      541 ccgcatcgtc acgacggcag tgccgttcgt cgtctgaacg ccggcgcgca agccgagaca
      601 gcgaacgccg attcgagtga ccattcggat gctcccgtgc tcccgtgctc ccgtgctcat
      661 ggctcgccgc gctggtggtg ggtgggccgc gatccgaagc cggctgcgtc gacgctgtct
      721 gggtcaggct ggcgttggag cggggcgcac catccattgg ccgatctcag agtcaccgag
      781 gcgtcggggc cgaaccgctc gcagagcccg tgtgcatacc gagtggccgg cgccagtcgg
      841 gcgttcacga tgtcagggtg tccggcggcc acttcgatga gccacgtacc cgcgccaatc
      901 gagcggtagt ggctcactgc gcacacatcg cagagccagg cgacgatcca cactgtcggt
      961 gacaagccga gcaaagcccg caaggccacc ggacggagca aacaggctga agcacagcga
     1021 gttgtcgaaa tccctcgcga tgatctctgg cgcgcggtct cgcgtccact ccgcgctcac
     1081 caccctgatc cgcgtcccct gcccctgacc cgcgccacac cgggcaatcg ggcttctgcg
     1141 gagtacggat ttgccggatt ccgggcagtc tccgatccgc ggacgccaca ttcaccgggc
     1201 gccgggtcag agggtgaagg cgcccgccgt agggcgggtt ggcgacgatg gcgcttcttc
     1261 tctgcggccg gggatggtgc gcccttcgcg acgggctctc ggtggaaact gggggccggt
     1321 gttgggtggg gttgggcaga ctgggatgcg ctgctgttac gtggggcggc ctgacaccat
     1381 gactcgtttc ttcacttctg atctgcactt cggtcacgcg aacgtgatcg ggtattgcgg
     1441 ccggccgttt cacggcgtcg ggcagatgaa cgcggcgatg gttgcggcgt ggaacacggt
     1501 cgttggcccc gacgacgagg tgtgggtgct gggagatctg gcgatgggga aactggacga
     1561 gtcgttgcag tgcgctgccc aactggtcgg gcgtaagcat ctggtgatcg gcaatcacga
     1621 ccgaccgttc cgtgggcacg gtgcggcgcg ttacgaggcg gtcggcttcg agttgcatca
     1681 cggtcacgtc gaactcgaca tgcccaacgg tgtcaccgtc gtggcctgcc actttccgta
     1741 caccggcgac tcgcacgacg tcgaccgtta cgacgaacat cgccccgccg acgagggccg
     1801 gtggttgttg cacgggcacg tgcacgagcg gtggcgccag cgcggccgga tgatcaacgt
     1861 cggcgtcgac gcctggggcg gctatccggt gaccgacacc cacctggcca ccctgatcga
     1921 cgccggcgaa gccgacctgc gtcccctggt gtgggaatag ccgcgagcgt gctcggtcgg
     1981 ccgcggtggt ccggggtcgg aactgagcat tgcactacga tgtttcgacg ctcggtggga
     2041 caacgggccg ctggatgtga agggcagatg tggggcatga gcaacgggat gtttcggggt
     2101 gtgcgccgcg gtcggggtgc cgtggtggtg gtgctcgtcg gggcgttgat cgccgcgtgc
     2161 ggtggggacg acaagggtgg ctcgaccacg actgcggcgt cgaccacaac gtctacagcg
     2221 gcgtcctcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng gtgaccaccg ccggcaacac
     2281 cggctcgggt gagttcacgc cgggtgacgt cacgtaccgg gtcgtcaacc tgctcgcggt
     2341 gccggtcgat ctgtacgccc gcacgcaggg ctttgtcgag gcctacccgt tgcagatcgg
     2401 actcgactcg ctgacggtca gcgagttcta cgcgccgccg gagaacggcc ggttcctggt
     2461 gacccaagcc ggcgccacgg acgtcacctg cgtgtcgtcg tgtaccgagt tcatcggtga
     2521 gttcgcgccg tatccagacg acggccccgt gcacacggtg gtggtctacg acgtcggcga
     2581 cggtcccaag acgttcgacc tgtgggagca ggcactgccg ccgtcgcagg gcaacgccaa
     2641 cgcgatggca ccggccgatg cgtcagcggg cctcgcgatc gtgaccgcga tcgccctcac
     2701 caactccgac ttcggtctgc ggttcgggtg ggccggggtc gacggatgtc acgagccggt
     2761 caacctggct ggcgttctcg tcggcggcaa ccagacgccg gcctacgtat atggcggcaa
     2821 tgccgccggc gtggtgctgt tccccaacga cgatcgtgat tgcgccggcg cgccggtcgg
     2881 ggggaccttc aacatctatg gcggggcggg cacccgcacc cacgtgatcc tgtacggcga
     2941 gcccgagaac atgtccgcgg tcgtgctgcc gatggcgggc gacgagacgg ccgccgccga
     3001 cgccgaagcc aacccggtgt acggcgaggc cgcgagcgga gatcccgctg ttcgcgacgc
     3061 tgtgattgct gccatggccg aagggcttgc gcgcgagatc gacttgacac ctgcgcaggc
     3121 ggcatgtgcc agcgagtatg tggtggacgc gctcgggctc gacgtggtgg cccccggcgg
     3181 cgtcgtggtc gacttcgaca gtctgggcga cgccgaggcc gagatcgcag gtgatgcgtt
     3241 ggtcgccgcg gtcgtgccgt gcggcatcga cccatccgtg ttgggctgag gtactgctcc
     3301 ccgccgacgg gcaaggtgcc accgcccgtc ggcagccgtg ggttagcttc gttaccgcat
     3361 gcgtggcgac ggagagcgag ggactggttc gtcgcgacca gagatcatga tgcgccaggc
     3421 gatggcagca atcatcgcgg cgatggcggc ggtcgggctg atcgcgctgg tcggcggcga
     3481 cgacagccct cgcccgacgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnncgaccca gccggatcga caacagcagg cccggctcct ggttcgtcgg ctgcggcagg
     3601 gaccgacccc tcgaatgcgg ggaccgcaag ctcggacgcg accgagacgt cggcgggtgg
     3661 cacggttgcg aacagcaccc cgacgaacag cgccccgacg agcaacagcg ttcccgcgac
     3721 cggtggctcg atggccagca gtcttgcggg agcggcgccg tcgggggtgg cgctgcatgg
     3781 cgatatcccc gcgggtgttg ggatgcccag cgtcgatgcc cgctcggtcg cggtctacga
     3841 cgcgacggcc ggacgatggc tgggtgggtc gaacgccgac gcccagatcg ccgtcggcag
     3901 cgtgatgaag ctgctcacct cgtacgtggt catgcaggcc ggcgatctgt cgaaggtggt
     3961 caccgtgccg gcgatggacg tcaacttcgc ggagtcatcg atcggtctgt accaaggcca
     4021 gaagctcgcc cgtgacgtgc tgctgcgggc gatgatgatc gtcagcgcca atgatgcggc
     4081 acgcgcgttg gcggtcgatg tcggcggcag caccgacaac ttcgttgcca agatgaacgc
     4141 ggccgccacg tcgctcgggc tggccaacac ccatgcggcc aacccgatcg gcctcgatgc
     4201 caccggggcc tactcgagcg cgcgcgacat ggtggctctc gccgagttgc tgatgcgtga
     4261 cgcggcgttc agcgcggcag tggcccgcac cagcgccaac atgcacggca ccaccttccc
     4321 ggccaccaac aagctgctcg gtagctacca gggtgccaac ggcgtgaaga ccggtcacac
     4381 cacgcaggcc ggctactgca tcgtcgcgtc cgccactcgt aacgggcgca cgatctacgt
     4441 cgcgttgttc ggagccgcgt cggatgcggc gcgcaccacc tcgacgacgg cgttgttcga
     4501 ctgggccttc gcccaagcct gagccccgat cctgcacccc gatcgtgccc cgatcctgac
     4561 cacgatcctg accttgatcc tgaccttgat cacgctggga actggtgacg tttgacccgt
     4621 cacccgggga cgggagcgtt cctgcacgga cgctaggttc ggggccatga agctcggtgc
     4681 gttcttcttc ggcggtgtgc agatgtccga tccgggaccg ggcgcgcccg acccgatgga
     4741 ccggcgctac tccaacgagg aggtgtgggc cgccaccaag tcgtacatcg acagcgccgt
     4801 ggtcgctgac gagctcggct acaactcgtt ctggaccacc gagcatcact tccagtacga
     4861 gggctacgaa gtgatcccca acggtgtgca gatctccacc tggatcgccg cgctcacgca
     4921 acagatcacg ctgggcacca tgttcaacgt cgttccgcag tggaacccgt tgcgcctggc
     4981 cgaagacttc tcgacgctgc tcaacctctc cggcggccgt gctgtgctcg gcgtcggccg
     5041 tggcacggtg ccgcgcgagg tgctgcacct caacgacaag ggtgtctcga tcgggtcgct
     5101 cgacaacccc gatcaggccg cagccgacgt gctcaaccgc gaggtgttcg aagagtcgat
     5161 ggagatcatc cgccgggcgc tgaacaacga gcggttctcc ttcagcggca agcacttcca
     5221 gatcccggta gcgggcatcc ccgatcgcgg caccacggtg caagagctga cgctggtgcc
     5281 ccggccgctg tatccgttcg agatctggca ggcggtcacc agcccaccga cgctcgacta
     5341 cgtgccggtg gtcggtcatg gcgcggtgtt ctggaaccag cactacgcgt tcatcaagcg
     5401 cttctgggac acgtacggcg ccaagtacga gcaggctcat ggcgaggcga tcgcgcccag
     5461 cgagaagcgg atgctggtcg tgtccgcccg tatcgaggac acccacgagc aggcgatggc
     5521 gtcggcccgc gccggccacg acgagttctg gaagttcctc gggccgtacg gctggagccg
     5581 cggctacatg ggtgacgacg gcaaaccggc caagccgggg ctgatcccca ccatggagga
     5641 gtcgatcgag aacaagacga tcctggtcgg ctcgcccgcc gacgtggccg ccgaggtgca
     5701 gttcctcaag gatctgctcg gcttcgagca cctgacgatc ttcccccatc tgctcggcga
     5761 ctcgtacgcc aaggccaacg agcagatgga gcgcttcatc accgaggtcg tcccgctggt
     5821 caactgacgg cccgccgacc ctggcacact gggccgatgg gttcggaatg ggacgatgat
     5881 ggcgacgtcg agccggacgt gcggttcttg tacgccaacg agcgcacgtt tctcgcgtac
     5941 aaccgcacct cgctcgcgct ggtcactgcg ggactcgcga tcactcagtt gctgccgccg
     6001 ttcgacatcc ccggcgggcg acggatgatc ggtctgccgt tgatcgcgat gggcaccgtc
     6061 atggcgctgg cgagcctgtt ccaatggaag cgcaacgaag cggcgatgcg ccgcggcgaa
     6121 tcgttgcccc ggtcgatctt gccgatcctg gtcaccatcg tggtcaccgc cagtgcggcg
     6181 gtggcgttga tcctggcgat cttcagcgac agcggcgcgt cgtgaagccg acgccccgat
     6241 ccgggcgc
//
LOCUS       DAORNF010000095         6237 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_9323_length_6237_cov_1.444193, whole genome shotgun sequence.
ACCESSION   DAORNF010000095 DAORNF010000000
VERSION     DAORNF010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 6237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6237
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9323_length_6237_cov_1.444193"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(9..>300)
                     /locus_tag="PLV68_05245"
     CDS             complement(9..>300)
                     /locus_tag="PLV68_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00681.1"
                     /translation="IFAAMGLIGVVARMRAPLQPPVATQLGAVREALRDHVVVLVEPE
                     RARLGQSLADQVVASLDVLCSYESYHLMRYDQGLSQDEATAAMTTGVIRLLA"
     assembly_gap    301..380
                     /estimated_length=80
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<381..>634)
                     /locus_tag="PLV68_05250"
     CDS             complement(<381..>634)
                     /locus_tag="PLV68_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HRE00682.1"
                     /translation="GMTVEAATEDDAQPARVQVDGRRGRRDRNRDAVVDALLTLYHDG
                     VLEPSADAIAEQAGLSARSLFRYFDDLDDLQRAAVERHAA"
     assembly_gap    635..782
                     /estimated_length=148
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <783..>1012
                     /locus_tag="PLV68_05255"
     CDS             <783..>1012
                     /locus_tag="PLV68_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00683.1"
                     /translation="PLVLDSLQRRMRALHSLYEDAVATMTIDHVNHFEREGVLPIAFC
                     LFHITNMMDASFMLMTGTPPIWNDAWQARVRMA"
     assembly_gap    1013..1143
                     /estimated_length=131
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1144..1339
                     /locus_tag="PLV68_05260"
     CDS             <1144..1339
                     /locus_tag="PLV68_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00684.1"
                     /translation="ELERVVITRPFPPQIASTYSARVAGPQGITLLDAAECWIYQHGL
                     RHMGEIELARGLVGLNGMTS"
     assembly_gap    1391..1490
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1491..2613
                     /locus_tag="PLV68_05265"
     CDS             <1491..2613
                     /locus_tag="PLV68_05265"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRE00685.1"
                     /translation="AIMGGFIVVDTLLGVRVFHVVIPALVAGLVVSYAVLLASRALLW
                     STVLAAAASIAVSAAVRAAEPTLYSPVMGLGAWPGVAELAGLGLLTGWSVRSLKLEGA
                     AIAVLSVAGALYAMADLRQDGPYRAGVQWLLAVGLAGAVGAGLYLRRIDDKQVQQTLQ
                     ARHDERLAIARELHDVVAHHVTGIVVQAQAAKLVADLQPEATIPALAAIETAGAEALT
                     AMRRMVGALREEDAAAPIAPAATLDDLRDIAERTTALGLPVRLHIDGDAAEVPGELAM
                     SIHRVVREALTNAQRHAIGATLVDVAVVIRDQSVRVIVRDNGHAGRPSKDRSGFGITG
                     MAERVSALGGRFRAGPLKSGGWQILADLPFAGVTSTMPT"
     gene            2645..>2853
                     /locus_tag="PLV68_05270"
     CDS             2645..>2853
                     /locus_tag="PLV68_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00686.1"
                     /translation="MTDTPSTDTPSTDIASTDAAAPTPRPLRVVIADDQALVRSGFRM
                     ILEAQGVTVVGEAGDGKTAVAIARET"
     assembly_gap    2854..2900
                     /estimated_length=47
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2901..3362
                     /locus_tag="PLV68_05275"
     CDS             <2901..3362
                     /locus_tag="PLV68_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRE00687.1"
                     /translation="ATRLLCGPDVTDPIPVVVVTTFDLDDYVRQAIRNGASGFLLKDA
                     GPALLLEAVRAAAAGDALVSPSVTVRFLQHFADAGHADSGARQPSIPLSEREEDVVRA
                     IARGLTNAEVGAELYISLSTTKTHVANIQNKLGARNRVEIASWAWQTGRMT"
     gene            3431..4279
                     /locus_tag="PLV68_05280"
     CDS             3431..4279
                     /locus_tag="PLV68_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00688.1"
                     /translation="MLSRRERLIAMRAIDIPTSIIRRFFQIDGMRMAMLMAFNLFISI
                     VPLAVIMFAFVSKVRSNLSISQVMIELFHLKGETARIVATAFPQTPNVLRMASVIAIV
                     SFAISGFDVASAFQRTFADAWNSERPRGWRGPARGGAWFLLVFATFAFGQLLQRGPAR
                     YGLVAYAITIPLIAFMNYWFWQLTPRLLLSKPLDNNDLRLGAVLGMIGSTALWWLSML
                     ILPGWFSWYGRGFGGIGIALAILSWTYVVSVVWVVIVVVSAVMWQRSATIDEVEEMAT
                     APSLRG"
     gene            complement(4301..>4685)
                     /locus_tag="PLV68_05285"
     CDS             complement(4301..>4685)
                     /locus_tag="PLV68_05285"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HRE00689.1"
                     /translation="ARAKEISGDGVDAVYDPIGGTVGEQCLRALRDDGQFLVIGFASG
                     AIPRLEANQVLLRNRRVTGVDWGGWVAKHPQRNAELTAEMFALITSGAVQPVEPAPYA
                     FDEAAVALADQQNRRIAGKAVLIVG"
     assembly_gap    4686..4740
                     /estimated_length=55
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4741..5270)
                     /locus_tag="PLV68_05290"
     CDS             complement(<4741..5270)
                     /locus_tag="PLV68_05290"
                     /inference="COORDINATES: protein motif:HMM:NF019845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HRE00690.1"
                     /translation="MRSVVCSSFAPLDQLQIVEQDSPPMKPGHVRVAVRACGVNFVDA
                     LMAQGLYQIKPPLPYVPGGESVGIVTEVADDVAAAGTGPALGQRVLVGGGIGGFTTEM
                     VVHATRAVAVPDVLTDTQAATFLQSYGTAYFALHERAKLAAGQWLLVLGAGGGVGLGA
                     VDVGHALGLQVIAAAST"
     assembly_gap    5495..5626
                     /estimated_length=132
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5627..5988
                     /locus_tag="PLV68_05295"
     CDS             <5627..5988
                     /locus_tag="PLV68_05295"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HRE00691.1"
                     /translation="IRRWSTDREADEVEIGGTWLAASAQRSAINTEAKLLMLAHAFDT
                     WGVQRVAICTDARNQQSRTAIERIGASFEGVLRNHRPSAVTAEEGQPRNTAIYSIIVD
                     EWPALRDTLRARLDGYA"
     gene            5981..>6237
                     /locus_tag="PLV68_05300"
     CDS             5981..>6237
                     /locus_tag="PLV68_05300"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRE00692.1"
                     /translation="MPDDSLPPSTTRDADDSQRVMLVTGGARGIGAEIARAGVARGYR
                     VAVTYSTSEIEATDLVNFAGVHAAIRSDAGEEADVVAAVAEV"
BASE COUNT          861 a         1968 c         1851 g          864 t
ORIGIN      
        1 gtcggcactc aggccagcaa gcggatcacg cccgtggtca tggcggccgt ggcctcgtcc
       61 tggctgagcc cttggtcgta gcgcatcaag tgatacgact cgtacgagca cagcacgtcg
      121 agtgacgcga ccacctggtc ggccaacgac tgtcccagtc gcgctcgctc cggttccacc
      181 aagaccacga cgtgatcgcg cagcgcctcg cgcacagcac ccagttgggt ggccaccggc
      241 ggctggagcg gagcacgcat gcgggcgacg acgccgatca aacccatcgc cgcaaagatc
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn gggctgcgtg acgttcgacc gcggcccgct gcaggtcgtc
      421 gagatcgtcg aagtagcgga acaacgagcg ggcagagagc ccggcctgct cggcgatcgc
      481 gtcggccgat ggttcgagca ccccgtcgtg gtacagcgtc agcaaggcat cgacgacggc
      541 gtcccggttg cggtcgcgcc gcccccggcg cccgtcgacc tggacacggg cgggctgcgc
      601 gtcatcctcc gtcgcagcct ccaccgtcat gcccnnnnnn nnnnnnnnnn nnnnnnnnnn
      661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      781 nncccctggt gctcgactcg ttgcagcggc gcatgcgcgc cctgcactcg ctgtacgaag
      841 acgccgtcgc caccatgacg atcgaccacg tcaaccactt cgaacgcgag ggcgtgctgc
      901 cgatcgcgtt ctgtctgttc cacatcacca acatgatgga cgcgtcgttc atgttgatga
      961 ccggcacgcc tcccatctgg aacgacgcct ggcaggcccg ggtgcggatg gcnnnnnnnn
     1021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnncgagttg gagcgggtcg tgatcacccg cccgttcccg ccgcagatcg ccagcacgta
     1201 cagcgcccgg gtcgccgggc cgcagggcat caccctgctc gacgcggccg agtgctggat
     1261 ctatcagcac ggcctgcgcc acatgggcga gatcgagttg gcccgcggcc tggtcggcct
     1321 caatggcatg acgtcctgac aacctgccgg gcgccgagct ggccgaggca ccgccgcttc
     1381 gccgctgcgg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cgcgatcatg
     1501 ggcggcttca tcgtggtcga cacgttgctg ggtgtgcgcg tgttccacgt ggtgatcccg
     1561 gcgctggtcg ccgggttggt cgtgtcgtac gcggtgctgt tggcgtcgcg ggcgctgctg
     1621 tggtcgacgg tgctggccgc cgctgccagc attgcggtgt ccgccgcagt gcgcgccgcc
     1681 gaaccgacgc tgtactcgcc ggtgatgggc ctgggagcgt ggccgggcgt ggccgagttg
     1741 gcaggcctcg ggctgctcac cgggtggagc gtgcgttcgc tgaagctgga gggcgccgcg
     1801 atcgcggtgc tgtcggtggc cggtgcgctg tacgcgatgg ccgatctccg ccaggacggg
     1861 ccctatcgcg ccggggtcca atggttgttg gcagtcggtc tcgccggggc tgtcggggcg
     1921 gggttgtacc tacgccgcat cgacgacaag caggttcaac agacgctgca agcacgccat
     1981 gacgagcgcc tcgccatcgc ccgcgaactg cacgacgtgg tcgcccatca cgtcaccggc
     2041 atcgtcgtgc aagcccaggc cgccaagctc gtcgccgacc tgcaacccga ggcaaccatc
     2101 cccgcgctgg ccgcgatcga gaccgccgga gccgaagcgc tgacggcgat gcggcgcatg
     2161 gtcggcgcgc tgcgagagga agacgcagcg gcaccgatcg cgccggcagc cacgctcgac
     2221 gacctgcgcg acatcgccga acgcacgacc gcactcggcc tgccggtgcg gctgcacatc
     2281 gacggcgacg cggccgaggt gccaggcgag ctcgcgatgt cgatccaccg cgtcgtgcgc
     2341 gaagcgctga ccaacgccca gcgccacgcg atcggggcca ccttggtcga cgtggcggtc
     2401 gtgatccgcg accagtcggt gcgcgtcatc gtccgcgaca acggccacgc cgggcgaccc
     2461 agcaaagacc gctcggggtt cgggatcacc ggcatggccg agcgcgtctc cgccctcggt
     2521 ggccgcttcc gggccgggcc gctcaagtct ggcggctggc agatcctcgc cgaccttccc
     2581 tttgccggtg tcacctcgac aatgcccacg tgagccaccg cgcccacccg atcggagcca
     2641 cgccatgacc gacaccccct ccaccgacac cccctcgacc gacatcgcct cgaccgacgc
     2701 tgcggccccc accccgcggc cgttgcgcgt cgtgatcgcg gacgatcagg ctctcgtccg
     2761 ctccgggttc cggatgatcc tggaggccca aggggtcacc gtcgtgggcg aggcgggtga
     2821 cggcaagacc gcggtcgcga tcgcccgcga aacnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn gcgacacggt tgctgtgcgg cccggacgtc accgacccga
     2941 tccccgtcgt ggtggtgacc accttcgacc tcgacgacta cgtgcgccaa gcgatccgca
     3001 acggagcgag cggcttcctg ctgaaagacg ccgggccggc gctgctgctg gaagccgtgc
     3061 gggctgccgc agcgggtgac gcgctggtgt caccctccgt gacggtgcgc ttcctgcaac
     3121 actttgccga tgccgggcac gccgactcgg gagcgcgcca gccgtcgatc cccctatcgg
     3181 agcgcgagga agacgtcgtg cgcgccatcg cccgcggcct gaccaacgcc gaggtcggtg
     3241 ccgagctgta catctcgctg agcaccacca agacccacgt cgccaacatc cagaacaagc
     3301 tcggcgcccg caaccgggtc gagatcgcca gctgggcctg gcagaccggc cgtatgacgt
     3361 gacccaatcg ctgcccccac cgttgccacc gttgcctccg cagccatcgt cgccgtctcc
     3421 gtcagagtcg gtgctgagcc ggcgcgagcg gctgatcgcg atgcgggcga tcgatattcc
     3481 gaccagcatc atccgccgct tcttccagat cgacggcatg cgcatggcga tgttgatggc
     3541 gttcaacctg ttcatctcca tcgtcccgct cgcagtcatc atgttcgcgt tcgtgtcgaa
     3601 ggtgcggtcc aacctgtcga tcagccaggt gatgatcgag ttgttccatc tgaaaggaga
     3661 gacggcacgc attgtggcga ccgcgttccc gcagacgccg aacgtgctcc gcatggcgtc
     3721 ggtgatcgcg atcgtttcgt tcgcaatcag cggcttcgac gtcgcctcgg cctttcagcg
     3781 cacctttgcc gacgcgtgga acagcgaacg cccgcgcggg tggcgcggcc cagcccgggg
     3841 tggggcgtgg ttcctgctgg tgttcgccac gttcgcgttc ggccagttac tccagcgcgg
     3901 tcccgcgcga tacgggctgg tggcctacgc catcaccatc ccgctgatcg cgttcatgaa
     3961 ctactggttc tggcagctga caccacggct gttgctcagc aagccgctcg ataacaacga
     4021 ccttcgcctc ggtgccgtac tgggcatgat cggcagcacc gcgctgtggt ggctgtcgat
     4081 gctgatcctg cccggttggt tcagttggta cgggcggggg ttcggtggca tcggcatcgc
     4141 gctcgcgatc ctgagctgga cctacgtcgt cagcgtggtg tgggtggtga tcgtggtcgt
     4201 gtccgcggtg atgtggcaac gctcggccac catcgacgag gtcgaggaga tggcgaccgc
     4261 gccaagcctg cgcgggtgac gacgcaaccc tcagcatccg tcagccgacg atgagcacgg
     4321 ccttgccggc gatccgccgg ttctgctgat cggccagcgc gacagcggcc tcgtcgaagg
     4381 catacggagc cggctcgacc ggctgcaccg cgcccgacgt gatcagcgcg aacatctccg
     4441 cggtcaactc ggcgttgcgc tgcgggtgct tggccaccca gccgccccag tcgacaccgg
     4501 tgacgcgccg gttgcgcagc agcacctggt tggcctccag ccgcgggatc gcgcccgacg
     4561 cgaaaccgat caccaagaac tggccgtcgt cgcgcagggc gcgcaggcat tgctcgccga
     4621 cggtgccgcc gatcgggtcg tagacggcat cgacgccgtc gccgctgatc tccttggctc
     4681 gggccnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4741 gtggatgctg ctgcgatgac ctgcaggccg agcgcgtggc cgacgtcgac cgctccgagc
     4801 cctacaccgc cgcccgcgcc aagcaccagc agccactggc cggccgcgag cttggcccgt
     4861 tcgtgcaggg cgaagtaggc cgtgccgtag ctctgcagga acgtggccgc ctgggtgtcg
     4921 gtgagcacgt cgggcacagc aaccgcccgc gtcgcgtgca cgaccatctc ggtggtgaag
     4981 ccgccgatcc cgccaccgac cagcacccgc tggcccagcg cggggccagt gcccgccgcg
     5041 gccacgtcgt cggccacctc cgtcacgatg ccgaccgact cgccacccgg cacgtacggc
     5101 aacggcggct tgatctgata cagcccttgt gccatcagcg cgtcgacgaa gttgacacca
     5161 caggcccgca ccgcgacacg cacatggccc ggcttcatcg gtgggctgtc ctgttcgacg
     5221 atctggagtt gatcgagcgg ggcgaagctg gaacagacca cggagcgcat gacggcgacc
     5281 gtagtagagc ccctctgtcg tcaccgacgc ctggtgagtc cacctggcgc cagcggccca
     5341 catcggccac gatgaacgaa tggtgtcgct gcaccccgcc gaatcgccga cgctcacagg
     5401 ccgtcacgtc caactcgaac cgctctccca cgatcacgtg ctgcagttgg tggtggccgc
     5461 caacgaagat cggtcgacgt acgggttgac cttcnnnnnn nnnnnnnnnn nnnnnnnnnn
     5521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnagat tcgcaggtgg
     5641 agcaccgacc gcgaagccga cgaagtggag atcggcggca catggctcgc cgcatcggct
     5701 cagcgcagcg cgatcaacac cgaggcgaag ttgttgatgt tggctcacgc cttcgacacc
     5761 tggggcgtgc agcgcgtcgc catctgcacc gacgcccgca accagcaaag ccgcacggcg
     5821 atcgaacgca tcggagcgtc gttcgaaggc gtgctgcgca accaccgccc ctcggcggtg
     5881 accgccgagg aaggccaacc gcgcaacacg gcgatctact cgatcatcgt cgacgaatgg
     5941 cctgcgctcc gcgacacgtt gcgggccaga ctcgacggtt atgcctgacg actcgttgcc
     6001 cccgtccacg acccgtgacg ctgacgacag ccagcgggtg atgctcgtca ccggaggggc
     6061 acgcggcatc ggtgccgaga tcgcccgcgc cggtgtggcc cggggctacc gcgtcgcggt
     6121 cacctactcg acgagcgaga tcgaagcgac cgacctggtc aacttcgccg gggtccacgc
     6181 cgcgatccgg tcggacgcgg gtgaagaagc ggacgtcgtc gccgccgtgg ccgaggt
//
LOCUS       DAORNF010000096         6210 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_9366_length_6210_cov_1.504143, whole genome shotgun sequence.
ACCESSION   DAORNF010000096 DAORNF010000000
VERSION     DAORNF010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 6210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6210
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9366_length_6210_cov_1.504143"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>313)
                     /locus_tag="PLV68_05305"
     CDS             complement(<1..>313)
                     /locus_tag="PLV68_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00693.1"
                     /translation="VRDGEVHHIGFNPVGLARTPLLRGRSRAGLLASMAKVMSVDPAR
                     WTGRPWSAYLGALAPDVAAIVSALARTSTYVDAPDTFDAGAALVQLKAGLRGVLYLDG
                     GW"
     assembly_gap    314..374
                     /estimated_length=61
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<375..628)
                     /locus_tag="PLV68_05310"
     CDS             complement(<375..628)
                     /locus_tag="PLV68_05310"
                     /inference="COORDINATES: protein motif:HMM:NF024842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRE00694.1"
                     /translation="MTLDASSQPDPVPSVTADLPAAADVVVVGGGIAGQIAAITVAAA
                     GRRVVLVEAQRPGGRARSTEREGFVYNTGPHALYPRFALYR"
     gene            complement(669..1136)
                     /locus_tag="PLV68_05315"
     CDS             complement(669..1136)
                     /locus_tag="PLV68_05315"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HRE00695.1"
                     /translation="MSIGVGDRAPGFTLPGTAPGSDATSFSLDDYAGRPVVLVFYPGD
                     DTPVCTKQLNSYNDGLAAFDELDAQVLAISAQDLTSKERFSGKHGFRFPLLADTDKAV
                     AASYGTLGPLGFPRRSVFVIDADGIIRYAHRAIAGLTYRPVGELVEAIRAASS"
     gene            complement(1144..>1484)
                     /locus_tag="PLV68_05320"
     CDS             complement(1144..>1484)
                     /locus_tag="PLV68_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRE00696.1"
                     /translation="GVIFVDGAADEDEVMMAALDAGADDISRDGDDWKVLTAPNTVYD
                     VQAALVAAGIAVKSAESTMVSSVSVDVTDVGDAKKILRIMDALEDDDDVQDVYSNFDI
                     SDELMDLVGE"
     assembly_gap    1485..1676
                     /estimated_length=192
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1677..1897)
                     /locus_tag="PLV68_05325"
     CDS             complement(<1677..1897)
                     /locus_tag="PLV68_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRE00697.1"
                     /translation="MSGHSKWATIKHQKGAKDAKRGKLFAKLARQIEVSARAGGADPA
                     SNATLRTAILKAKAAQMTNDAIDRAAKRGA"
     assembly_gap    1989..2084
                     /estimated_length=96
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2085..>2386)
                     /gene="pdxT"
                     /locus_tag="PLV68_05330"
     CDS             complement(<2085..>2386)
                     /gene="pdxT"
                     /locus_tag="PLV68_05330"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR03800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HRE00698.1"
                     /translation="VRVAADLDGIDALVMPGGESTTMSKLLDTSGLRDPLAAAVRAGL
                     AVFGTCAGMILLATEIIDGRPDQVGLDAIDIAVRRNGYGRQVDSFEADLDVAGLTEP"
     assembly_gap    2387..2464
                     /estimated_length=78
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2562..>2828)
                     /locus_tag="PLV68_05335"
     CDS             complement(2562..>2828)
                     /locus_tag="PLV68_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HRE00699.1"
                     /translation="ELPVPLFCAGGIATPADAALAMQLGAQAVFVGSGIFKSADPAPR
                     AKAIVEATTHFNDAGILAKVSRGLGTPMTGIGMDDVVKFAERGW"
     assembly_gap    2829..2913
                     /estimated_length=85
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2914..3258)
                     /locus_tag="PLV68_05340"
     CDS             complement(<2914..3258)
                     /locus_tag="PLV68_05340"
                     /inference="COORDINATES: protein motif:HMM:NF013815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HRE00700.1"
                     /translation="MSDPEMIEGIQAAVSIPVMAKVRIGHFVEAQILQAMGVDFIDES
                     EVLTPADETHHVDKFAFDVPFVCGATNLGEALRRIAEGACMIRSKGEAGTGNIVEAVR
                     HLRSIRGDLRKIT"
     assembly_gap    3274..3400
                     /estimated_length=127
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3530..>4469)
                     /locus_tag="PLV68_05345"
     CDS             complement(3530..>4469)
                     /locus_tag="PLV68_05345"
                     /inference="COORDINATES: protein motif:HMM:NF022702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HRE00701.1"
                     /translation="TTTTVADDVARLPLRGTPVPFGFDGTRPAMVVKLDNHPDARPQS
                     GLNEADIVFEENVESLTRFAAVFHSTDANPVGPIRSGRTQDVELLAPFLNPMFVWSGG
                     NARVTQAIRGSGMFQIDQTVGLGVMYRRRGRNNPHDLYADMSKLYGLRPDSALPPPAQ
                     FSYLADDTEASGDQATGVKVSMDGVRVLWEWDAASSTYLRQTDGKAHKDDNGGAQWST
                     ENVVVLTVVYRQSAADVRSPEAQTTGSGDVVVLSGGRVRTGRWTRDNQLVPFSLTDAD
                     GAVIELTPGRTVVELARANKTAVVPAGTAVADVAYP"
     assembly_gap    4470..4479
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4480..4673)
                     /locus_tag="PLV68_05350"
     CDS             complement(<4480..4673)
                     /locus_tag="PLV68_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00702.1"
                     /translation="MLGWAASATGLAAVVAACGGDDKAADTTAPPIVTLDRPTTTEPT
                     TTLPEATTTSYSSSDSGRPAP"
     gene            complement(4736..5641)
                     /locus_tag="PLV68_05355"
     CDS             complement(4736..5641)
                     /locus_tag="PLV68_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HRE00703.1"
                     /translation="MFDLIRSNKRKSVALVAGFVVVVVAVGWAIGLLIGNGIVATIVA
                     LVIAAVMSVVSYWKADAIALRVSRAVPADPQQYQRLHNLVEGLCIASGLPKPRVYIVD
                     DPAPNAFATGRDPKHAAIAVTTGLLEKMNRVELEGVLAHELSHIRNYDILVSTLVVTL
                     VGAIAILTDLGIRLMWWNGGRVRRDGDRNDSGNPLALVGFALLIFAPLLAKVMQAAVS
                     RRRETLADVSACQMTRYPPGLISALEKLRDDTTVTHSASTATAHLWIEQPLSGVGDQG
                     RLSSLHRMFDTHPPLEERIALLREL"
     gene            complement(5651..>5870)
                     /locus_tag="PLV68_05360"
     CDS             complement(5651..>5870)
                     /locus_tag="PLV68_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HRE00704.1"
                     /translation="KANTNFLALQEELTATEGRVAYARQFYNDSVLTYNNAIQQFPSV
                     IFASMLKFQRREYFEADAGSREAPRVQF"
     assembly_gap    5871..5919
                     /estimated_length=49
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5920..6207)
                     /locus_tag="PLV68_05365"
     CDS             complement(<5920..6207)
                     /locus_tag="PLV68_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HRE00705.1"
                     /translation="MIRDFGVLLAVGIVVLLALMGVVIYNRLIARRNQVENAWSQIDV
                     QLKRRIDLIPNLVETVKAYAAHERETLDAVVRARNAAIAAPGTPSAQAAADT"
BASE COUNT          868 a         1831 c         1927 g          886 t
ORIGIN      
        1 gccaaccccc gtcgaggtac agcacgccgc gcagacccgc cttgagctgc accagcgccg
       61 caccggcgtc gaacgtgtcg ggcgcatcga cgtaggtcga cgtgcgcgcc agcgcggaga
      121 cgatcgcggc gacgtcgggc gcgagcgcac ccagatacgc cgaccacggc cggcccgtcc
      181 atcgtgccgg gtcgacgctc atcaccttgg ccatgctcgc caacagtccc gcccggctcc
      241 gcccgcgcag caacggtgtg cgggcgaggc cgaccggatt gaagccgatg tggtgcacct
      301 cgccatcgcg gacnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnncggtag agcgcgaagc gcgggtacag cgcgtgcggc cccgtgttgt
      421 agacgaatcc ttcacgctcg gtcgagcgag cacgaccccc cgggcgttgc gcctcgacca
      481 acaccacgcg ccgccctgcg gcagcgacgg tgatcgccgc gatctgtccg gcgatgccac
      541 cgccgacgac cacgacgtcc gccgcagcgg gaaggtcggc tgtaaccgac ggcacgggat
      601 ctggttggga cgaggcgtcg agtgtcatga ctgttcgacg tcgcgggcgg cgagtttgtg
      661 acagccggct aactcgacgc ggcgcggatc gcttcgacca gctcaccgac cgggcggtag
      721 gtgagcccgg cgatggcccg gtgggcatag cggatgatgc cgtcggcgtc gatcacgaac
      781 acgctgcggc gggggaagcc gagcgggccg agggtgccgt agctggcggc aaccgccttg
      841 tcggtatcgg ccagcagcgg aaaccggaag ccgtgcttgc ccgagaaacg ctccttgctg
      901 gtgaggtcct gggccgagat cgccagcacc tgtgcgtcga gttcgtcgaa ggccgcgagc
      961 ccgtcgttgt acgagttgag ctgcttcgtg cacaccggcg tgtcgtcgcc cgggtagaac
     1021 accaacacga cgggccgacc tgcgtagtcg tccagcgaga agctggtcgc gtcggaaccg
     1081 ggagcggtac ccggcagcgt gaagccgggc gctcggtcac ccaccccgat gctcatcgcg
     1141 gcgtcactcc cccaccagat ccatcagctc gtcggagatg tcgaagttgg agtacacgtc
     1201 ctgcacgtcg tcgtcgtctt ccaacgcgtc catgatcctc aagatcttct tggcgtcgcc
     1261 cacgtcggtg acgtcgaccg acaccgacga caccatcgtg ctctcggccg acttcacggc
     1321 gatgccagcg gctaccagcg cggcctgcac gtcgtacacg gtgttggggg cggtgagcac
     1381 cttccagtcg tcgccgtcgc ggctgatgtc gtcggccccg gcatcgagcg cggccatcat
     1441 cacctcgtcc tcgtccgcgg ctccgtcgac gaagatcacg ccacnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngcac
     1681 cgcgcttggc ggcccgatcg atggcgtcgt tggtcatctg cgccgccttg gccttcaaga
     1741 tcgccgtgcg cagcgtggcg ttgctggccg gatcggcgcc accggcgcgg gccgagacct
     1801 cgatctggcg ggccagcttg gcgaacagct tgccgcgctt ggcgtctttg gcacccttct
     1861 ggtgcttgat ggttgcccat ttcgaatggc cggacatggc tcagacctcc tggaggaaca
     1921 aggcgtgcag tcgtggatcg ggagtgagtt cggggtggaa cgaggcgacg acgatgcgcc
     1981 cgttgcgcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnggttcg gtcagcccgg
     2101 cgacgtcgag gtcggcttcg aagctgtcga cctggcggcc atacccgttg cggcgcaccg
     2161 cgatgtcgat cgcgtcgagg ccgacctgat cgggacggcc gtcgatgatc tcggtcgcga
     2221 gcaagatcat gcctgcgcag gtgccgaaca cggccaaccc ggcgcgcacc gccgccgcca
     2281 agggatcgcg cagacccgac gtgtcgagca gcttcgacat ggtggtgctt tcgccgccag
     2341 gcatcaccag ggcatcgatg ccatcgaggt cggccgcgac gcggacnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnacgccg acggtgcgcg gcgcagcgga ggtctcagcc ggggcgacgg ccgttgcggc
     2521 cgggtgcacc aggaggctcc gttctggcgt tgctcacgcg gtcaccaacc ccgctcggca
     2581 aacttgacga cgtcgtccat gccgatgccg gtcatgggcg tacccaggcc ccggctgacc
     2641 ttggccagga tgcccgcatc gttgaagtgc gtggtcgcct cgacgatcgc cttggcgcgc
     2701 ggtgccgggt cggcgctctt gaagatgccc gagccgacga acacggcctg ggcaccgagc
     2761 tgcatcgcca gcgccgcgtc ggccggagtg gcgatgccac cggcacagaa caacggcacc
     2821 ggcagctcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnggtgatc ttgcgcaggt cgccccggat
     2941 cgaacgcagg tgacgcacgg cttcgacgat gttgcccgtg ccggcctcgc ccttggagcg
     3001 gatcatgcac gcgccttcgg cgatgcgccg cagggcttcg ccgaggttgg tggcgccgca
     3061 cacgaacggc acgtcgaacg cgaacttgtc gacgtggtgc gtctcgtcgg ccggggtgag
     3121 cacctcgctc tcgtcgatga agtcgacccc catcgcctgc aggatctgcg cttcgacgaa
     3181 gtggccgatg cgcaccttgg ccatgaccgg gatggagacc gcggcctgga tgccttcgat
     3241 catctcggga tcgctcatgc gcgccacccc gccnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn caaaccccgc ttgacggggt
     3421 aggtaccggt ggcgcgcgtc gaattcatga acgccaatct agcgaccgga cccgaccagt
     3481 ccctcagagt tcaccgaggt cagcgcccac tcgacgtgag cgatgtgcgt cagggatagg
     3541 cgacgtcggc cacggcggtt ccggcaggca cgacggcggt cttgttggcc cgggccagct
     3601 cgaccaccgt gcggccgggg gtgagctcga tcaccgcacc atcggcgtcg gtgaggctga
     3661 acggcaccag ttggttgtct cgggtccagc gcccggtgcg cacccgtccg ccgctgagca
     3721 cgaccacatc accagaaccg gtggtctgcg cctcggggct ccgcacgtcg gccgcgctct
     3781 ggcggtagac gacggtgagc acgaccacgt tctcggtcga ccactgagcg ccgccgttgt
     3841 cgtccttgtg ggccttgccg tcggtctggc gcaggtacgt ggacgaagcg gcatcccatt
     3901 cccacaacac gcgaacaccg tccatcgaca ccttgacccc agtggcttgg tcacccgacg
     3961 cttcggtgtc gtcggcgagg tagctgaact gggccggggg tggcaacgcc gagtcgggtc
     4021 gcaagccgta cagcttggac atgtcggcgt agagatcatg ggggttgttc cgtccccgac
     4081 gtcgatacat gacgccgagg ccgacggtct ggtcgatctg gaacatgccg ctaccgcgga
     4141 tggcctgggt gacccgggcg ttgccgccgc tccacacgaa catcgggttg aggaacgggg
     4201 ccaggagttc gacgtcttgc gtgcgccccg aacggatcgg cccgaccggg ttggcatcgg
     4261 tactgtggaa cacggccgcg aaacgggtca agctctcgac gttctcttcg aacacgatgt
     4321 cggcctcgtt gaggcccgac tgcggccggg catcggggtg gttgtcgagc ttgaccacca
     4381 tcgccgggcg ggtgccgtcg aagccgaacg gcacgggtgt gccgcgcaac gggagccggg
     4441 ccacgtcgtc ggccacggtc gtggtggtcn nnnnnnnnng gcgcgggcct gccgctgtcg
     4501 gacgacgaat acgacgtggt ggtcgcctcg ggcagcgtcg tcgtcggctc ggtggtggtc
     4561 ggccggtcca gcgtcacgat cggcggcgca gtggtgtcgg ccgccttgtc gtcgccacca
     4621 caggcggcaa ccaccgcggc caaaccggtt gccgacgccg cccagcccag cacctcccgc
     4681 cggttgaggt tgcgactgct gcgatcgggg tggcgggcag cgggatcggg tcggttcaca
     4741 gttccctcag caaggcgatg cgctcttcga gcggcgggtg cgtgtcgaac atgcggtgca
     4801 ggctcgacag acggccttgg tcgccgacgc ccgacagcgg ttgttcgatc cacagatggg
     4861 ccgtggccgt ggaggccgag tgggtgacgg tggtgtcgtc gcgcaacttc tccaacgccg
     4921 agatcagccc tggcgggtag cgagtcatct ggcacgcgga cacatcggcc agtgtctcac
     4981 gccggcggct gaccgcggcc tgcatcacct tggcgagcag cggggcgaag atcaacagcg
     5041 cgaagcccac caacgcgagc gggttgccgc tgtcgttgcg gtcgccgtcg cgccggacgc
     5101 gcccgccatt ccaccacatc aggcggatac cgagatcggt caagatcgcg atcgcgccca
     5161 ccagggtgac gaccagggtg gagacgagaa tgtcgtagtt gcggatgtgc gacagctcgt
     5221 gggccagcac cccttcgagc tcgacccggt tcatcttctc gagcaggccg gtggtgacgg
     5281 cgatggccgc gtgcttggga tcgcggccgg tggcgaacgc gttgggcgcc ggatcgtcga
     5341 cgatgtacac ccgcggtttg ggcaggccac tggcgatgca cagaccctcg accaggttgt
     5401 gcagccgctg atactgctgc gggtcggcgg gcacggcccg gctgacccgc aacgcgatcg
     5461 cgtcggcctt ccagtacgac accaccgaca tgaccgcagc gatcaccaac gcgacgatgg
     5521 tggcgacgat gccgttgccg atcaacaggc cgatggccca cccgacggcg acgaccacca
     5581 cgacgaagcc ggcgacgagc gcgacgctct tgcgtttgtt ggaacgaatg agatcgaaca
     5641 tcgggggtga tcagaactgc acgcgcggcg cctcgcgcga accggcgtct gcttcgaagt
     5701 actcacgccg ttggaacttg agcatgctgg cgaagatgac gctggggaac tgctggatcg
     5761 cgttgttgta cgtcaacacg ctgtcgttgt agaactggcg ggcgtaagcg acgcggccct
     5821 cggttgcggt gagctcttcc tgcagcgcga ggaagttggt gttggccttc nnnnnnnnnn
     5881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng gtgtcggcgg cagcctgggc
     5941 cgacggtgtg cccggcgcgg cgatggcggc gttgcgagcc cgcaccacag catcgagcgt
     6001 ctcccgctcg tgggccgcgt aggccttgac ggtctcgacc aggttgggga tgaggtcgat
     6061 gcggcgcttc aactgcacgt cgatctgcga ccaggcattc tcgacctggt tgcgccgggc
     6121 gatgaggcgg ttgtagatga cgacacccat gagcgccagc aacacgacga tgccgaccgc
     6181 cagcagcacg ccgaagtcgc ggatcatcgg
//
LOCUS       DAORNF010000097         6200 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_9388_length_6200_cov_1.599024, whole genome shotgun sequence.
ACCESSION   DAORNF010000097 DAORNF010000000
VERSION     DAORNF010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 6200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6200
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9388_length_6200_cov_1.599024"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..872
                     /locus_tag="PLV68_05370"
     CDS             <1..872
                     /locus_tag="PLV68_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00706.1"
                     /translation="GRVCVYTHTGADYALDVNGYVPSGSPVELARPARLLDTRRDSWA
                     TTVDGRHQGTGVVRAEQTVEIPIAGRGDVPRGATAALVTVTAVDPAAAGFLTVYPCGS
                     RPHASSVNFAAGQTVPNGVIAELSASGSLCVYTNAATDLVVDVTGSVTGNGTGLGTFV
                     PERLVDTRPDGPTIDGHNSGTPSRLRQGTTIEVQVAGRASVPADATAALLNIGLVAPD
                     NTTFATLYPCGERPTASNVNLVAATSTRANSTVTKLSPSGTVCIYVHAATDLILDITG
                     YLQHAEPAPRQST"
     gene            complement(860..>1072)
                     /locus_tag="PLV68_05375"
     CDS             complement(860..>1072)
                     /locus_tag="PLV68_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00707.1"
                     /translation="QATADEDVWTMSSAVDPDSDWVVVEFSPVTPTDYLDFSTLDVFI
                     DVVGNGGVYRESLDQAIGCAVDVYVD"
     assembly_gap    1073..1094
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1230..1427
                     /locus_tag="PLV68_05380"
     CDS             1230..1427
                     /locus_tag="PLV68_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00708.1"
                     /translation="MQELSMVPVPTDEEAVAIAAAVQAMWPQPVVAADAAVPGRDTAW
                     KFSGRWWSRPLPNRRDRPFYR"
     assembly_gap    1465..1501
                     /estimated_length=37
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1502..2475
                     /locus_tag="PLV68_05385"
     CDS             <1502..2475
                     /locus_tag="PLV68_05385"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRE00709.1"
                     /translation="HRYDDLRPATTYAFGHAGGEIAVTTLSRPPGELLCRFATVNDVH
                     FGEVEAGRLGKHQMGPIRRAEPGEAPYPETMNAGAVVEMTAIDPIAVFAKGDLTADGT
                     PDQFAAFERVYRSAFGERLYAVRGNHDAYMFQTDYAGDRWVTVPGMAVALLDTTTPGA
                     TPGSIGAEQFEWLDTLAAESDVPVLVMGHHPQWLAHATEPHTRDGDTFSLDRPLSEAL
                     AEVCARRRSIVGYTAGHTHRHRNRPMLADGTMVTVEVGCVKDFPGTWAEYRVYEGGAM
                     QVIHRISTADALSWSERCRHLYADFGIDYETYAMGHLEDRCFLIPYR"
     gene            2472..>2893
                     /locus_tag="PLV68_05390"
     CDS             2472..>2893
                     /locus_tag="PLV68_05390"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRE00710.1"
                     /translation="MNAPQGRLRTSLRAANVPPFHAMAMARLASEREAAGHPVHHLEV
                     GQPSTGAPAKAVTAGVAAMQSQPVGYTNAAGLLSLRRRIAAHYADWYGIEVPTERILV
                     VAGASAGFTLAFMACFDIGQRVGIVEPGYPCYRNVLTA"
     assembly_gap    2894..3033
                     /estimated_length=140
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3034..>3271
                     /locus_tag="PLV68_05395"
     CDS             <3034..>3271
                     /locus_tag="PLV68_05395"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRE00711.1"
                     /translation="IATGSRQFGTRLIADEIYHGITYGGPAPTALAHTDQAVVINSFS
                     KYFSMTGWRLGWVVVPDELVDPFERLQQNLYICAP"
     assembly_gap    3272..3383
                     /estimated_length=112
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3384..3608
                     /locus_tag="PLV68_05400"
     CDS             <3384..3608
                     /locus_tag="PLV68_05400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00712.1"
                     /translation="VAEPDGAFYVYADVSHLSDDSMTLCHRWLTEIGVATTPGLDFDT
                     ARGHRFVRFSYAGQAASIAAACEALTRWQP"
     gene            3605..>4140
                     /locus_tag="PLV68_05405"
     CDS             3605..>4140
                     /locus_tag="PLV68_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRE00713.1"
                     /translation="MTANDATANGPTANDGAENHVPANDMPTNDVPARSGPLSDLRVI
                     DLSTVIAGPKCAAYLADFGADVIKVERPGSGDTLRNMAWRDPRDDQGMWWKLVNRNKR
                     TIALDLKDHTDRDLLIDLVREANVLVENFRPGALERLGLGPDELLAINPRLVITRITG
                     FGQTGPYKQRAGFATIAEA"
     assembly_gap    4141..4199
                     /estimated_length=59
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4200..4832
                     /locus_tag="PLV68_05410"
     CDS             <4200..4832
                     /locus_tag="PLV68_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRE00714.1"
                     /translation="PPIALTDEVTAIGAALATMIAVHSGVGQVVDVSLLETMFQIMGP
                     LVSLYALTGEMQPRLGSGLPYTVPRGTYQCADGGWVGVSSSSDSVARRVTALLGVGDD
                     ERFATFAARVAHRDELESLMTEWCRARPRDEVLRLFTEAEAAIGPVLDMADIAADPHY
                     AARDAVVTVGDTPMQGVFAKLSATPGVLRWSGRSIDADGVSIRATKWGSG"
     gene            complement(4824..5150)
                     /locus_tag="PLV68_05415"
     CDS             complement(4824..5150)
                     /locus_tag="PLV68_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00715.1"
                     /translation="MSGFMDKAKDLAEDIKDKAEDVVDKIEDKIPDSVKEKFSAVKDK
                     AGDIVDKIEDKIPDSVKDKVSAVTDKIKDIIPGGDDKDDDDDDKDDAAATAADAEAAP
                     SDDASA"
     gene            complement(5147..5803)
                     /gene="msrA"
                     /locus_tag="PLV68_05420"
     CDS             complement(5147..5803)
                     /gene="msrA"
                     /locus_tag="PLV68_05420"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HRE00716.1"
                     /translation="MFFKKAEMIAADEALPGRTDQTMPVPDAHFVTGHPLVGPWPDGY
                     QTAVFGLGCFWGAERKFWQTAGVYSTASGYAGGYTPNPTYEEVCSGRTGHTEVVQVVF
                     DPTAVSYEELVKLFWESHDPTQGYRQGNDVGTQYRSAIYTTSPAQAAQAQASQAMYQA
                     ALDAKGYHTITTEIAPLDHFYYAEPYHQQYLGKNPNGYCGLGGTGVSCPVGLTGNVPS
                     "
     assembly_gap    5813..5909
                     /estimated_length=97
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5962..>6200)
                     /locus_tag="PLV68_05425"
     CDS             complement(5962..>6200)
                     /locus_tag="PLV68_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HRE00717.1"
                     /translation="LALKHGVTVANAPGLIDAAYRGEIKVVLLNTDPAEAYTVQRGDR
                     IAQLVLQRVEHVVWQSTTTLDGHDRGGGFGHSGR"
BASE COUNT          933 a         1994 c         1888 g          918 t
ORIGIN      
        1 agggccgggt ctgcgtctac acccacaccg gcgccgacta cgcgctcgac gtgaacggat
       61 acgtaccgag cggctcgccg gtggaactgg cccgtccggc tcgactcctg gacacgcgac
      121 gcgactcatg ggcgacgacc gtcgacggtc gtcatcaggg caccggagta gtcagggcgg
      181 aacagaccgt cgagattccc atcgccggtc gtggcgacgt ccctcgcggt gcgaccgctg
      241 cgttggtgac ggtgaccgcc gtcgatccag cagcagcggg cttcctcacg gtgtacccgt
      301 gcggtagccg tccccacgcc agctcggtca acttcgccgc ggggcagacc gtccccaacg
      361 gcgtgatcgc cgagctctca gcgtcgggat cgctgtgcgt ctacaccaat gccgccaccg
      421 acctcgttgt cgacgtcacc ggctcagtca ccggcaacgg caccgggctc ggcacgttcg
      481 tacccgagcg gctggtcgac acccgacccg atggaccgac gatcgacggg cacaactcgg
      541 gaacaccgtc gcggctgcgt cagggcacga cgattgaagt gcaggtcgcc gggcgcgcca
      601 gcgtgcccgc cgacgcgacc gcggcgctgc tcaacatcgg cctcgtcgct ccggacaaca
      661 ccactttcgc cacgctgtat ccatgcggtg agcggccgac ggcctcgaac gtcaaccttg
      721 tcgccgcgac atcgacccga gcgaacagca ccgtgacgaa gctctcaccg tccgggacgg
      781 tgtgcatcta cgtccacgcc gccaccgacc tcatcctcga catcaccggc tacctccagc
      841 acgccgagcc ggcgccgcgt cagtccacgt agacgtcgac ggcgcagccg atcgcctgat
      901 ccaacgactc gcggtacacc ccgccattcc ccacgacgtc gatgaagacg tcgagggtcg
      961 agaagtcgag gtagtcggtc ggggtcaccg gcgagaactc caccacaacc cagtccgagt
     1021 cagggtccac cgcactcgac atggtccaga catcctcgtc agcagtggcc tgnnnnnnnn
     1081 nnnnnnnnnn nnnngacggt acgacgcgaa cgctcccacg gcccatacgg cccgagcagc
     1141 ctctgcggtg gtggtgccgg gaacgtcacg ttgccgttga tgggtacgcc gtcgcggctg
     1201 agcgaaccgg gcacgggtag ggtgggggcg tgcaggagtt gtcgatggtg ccggtgccga
     1261 cggacgagga ggcggtggcg atcgccgctg cggtgcaggc gatgtggccg cagccggtgg
     1321 tggcggccga cgccgctgtg ccgggtcgcg acacggcgtg gaagttcagc ggccggtggt
     1381 ggtcgcgtcc gctgccgaac cggcgcgatc gccccttcta tcgctgaccg tcaccgcacc
     1441 agcggtaccg tgccccagat ggacnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 ntgcaccgtt acgacgacct gcggcccgcc acgacgtacg ctttcgggca cgccggtggc
     1561 gagatcgccg tcaccaccct gtcccgtccg ccaggcgagt tgctgtgccg tttcgccacc
     1621 gtcaacgacg tccacttcgg cgaggtcgaa gcagggcgcc tcggcaaaca ccagatgggg
     1681 ccgatccgac gcgccgaacc cggtgaagcc ccctacccgg agacgatgaa cgccggcgcg
     1741 gtcgtcgaga tgacggcgat cgacccgatc gcggtgttcg ccaaaggtga cctcaccgcg
     1801 gatggtacgc cggaccagtt cgccgccttc gagcgcgtct atcgctcggc cttcggcgag
     1861 cggttgtacg cagtgcgcgg caaccacgac gcgtacatgt tccaaaccga ctacgccggt
     1921 gatcggtggg tgacggttcc gggcatggcc gtggcgctgc tcgacacgac gactcccggc
     1981 gcgacccctg gcagcatcgg tgccgaacag ttcgaatggc tcgacacgtt ggcggccgag
     2041 agcgacgtcc cggtgttggt gatgggacac cacccgcaat ggctcgctca cgccaccgag
     2101 ccgcacactc gcgacggaga cacgttcagc ctcgaccgtc cgctcagcga ggcactcgcc
     2161 gaggtgtgtg cgcggcgacg atcgatcgtc ggctacaccg ccggacacac ccaccggcat
     2221 cgcaaccgcc cgatgcttgc agacggaacg atggtcaccg tcgaagttgg ctgcgtcaag
     2281 gactttcccg gcacatgggc cgagtaccgg gtgtacgagg ggggcgcgat gcaggtgatc
     2341 caccgcatct ccaccgcgga cgcgctgtcg tggagcgagc gctgccgcca cctctacgcc
     2401 gacttcggca tcgactacga gacctacgcc atgggccacc tggaggatcg atgcttcctg
     2461 atcccgtatc gatgaacgcg ccgcagggac gcctgcgcac ttcgctgcgc gccgccaacg
     2521 tgccgccgtt tcacgccatg gcgatggccc ggctggccag cgagcgcgaa gccgccggtc
     2581 atcccgtgca ccacctggag gtcggtcaac cgtccaccgg cgccccggcc aaagccgtga
     2641 cggccggggt ggccgcgatg cagtcacaac ccgtcggcta caccaacgcg gccggattgc
     2701 tctcgctgcg gcgacggatc gcggcgcact acgccgactg gtacggcatc gaggtgccga
     2761 ccgaacgcat cctggtcgtc gccggggcat cggccgggtt caccttggcg ttcatggcct
     2821 gcttcgacat cggccagcgc gtcggcatcg tcgaaccggg ctacccgtgc taccgcaacg
     2881 tgctcaccgc gttnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnatcgcca cggggtcccg gcagttcggc
     3061 acgcggctga tcgccgacga gatctaccac ggcatcacct acggcggacc cgcgccgacc
     3121 gccctcgcgc acaccgacca ggcggtcgtg atcaacagct tctccaagta cttcagcatg
     3181 accggctggc gcctgggttg ggtcgtggta cccgacgaac tggtcgaccc attcgagcgg
     3241 ttgcagcaga acctctacat ctgcgccccg cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnngtcgcgg aacccgacgg cgcgttctac gtctacgccg
     3421 acgtctccca tctcagcgac gattcgatga cgctgtgcca ccgctggctc accgagatcg
     3481 gcgtcgccac caccccaggg ctcgacttcg acacggcccg cggccaccgc ttcgtccgct
     3541 tctcgtacgc aggccaggcc gcctcgatcg cggcggcatg cgaagcactg acacggtggc
     3601 agccgtgacc gcaaacgacg cgaccgccaa cggcccgacc gcgaacgacg gagcagagaa
     3661 ccacgtgcca gcaaacgaca tgcccacaaa cgacgtgcca gcgcgttcag gtccgttgtc
     3721 ggacctgcgc gtgatcgatc tgtcgaccgt gattgctggg ccgaagtgcg cggcctactt
     3781 ggccgatttc ggtgccgatg tgatcaaggt cgagcggccc ggtagcggcg acaccctgcg
     3841 caacatggcc tggcgcgacc cccgcgacga ccaaggcatg tggtggaagt tggtcaaccg
     3901 caacaagcgc accatcgcac tcgacctcaa ggaccacacc gatcgcgacc tgctcatcga
     3961 cctggtgcgc gaagccaacg tgttggtcga gaacttccgg ccgggcgcgc tggaacggct
     4021 cggcctcggc cccgacgaac tgttggcgat caaccctcgc ctggtgatca cgcggatcac
     4081 cgggttcggc cagaccgggc cgtacaagca gcgagccggg ttcgcgacca ttgccgaggc
     4141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc
     4201 cgccgatcgc gctgaccgac gaggtgaccg cgatcggtgc cgcactggcg acgatgatcg
     4261 cggtgcacag cggcgtcggt caggtggtcg acgtcagcct gctggagacg atgttccaga
     4321 tcatgggtcc gctcgtatcg ctgtacgcgt tgaccggcga gatgcaaccg cggctcggct
     4381 ccggcctgcc gtacaccgtg ccgcggggca cctaccagtg cgccgacggc ggatgggtgg
     4441 gggtcagttc cagcagcgac agcgtcgcgc ggcgggtgac cgcactgttg ggcgtgggcg
     4501 acgacgaacg cttcgccacc ttcgccgcgc gggtcgccca ccgcgacgaa ctggaatcgc
     4561 tgatgaccga gtggtgccgg gcgcgtccac gcgacgaggt gctccgcctg ttcaccgaag
     4621 ccgaggccgc gatcgggccg gtgttggaca tggccgacat cgcggccgac ccgcactacg
     4681 ccgcgcgcga cgcggtcgtg accgtgggtg acaccccgat gcaaggggtg ttcgccaagc
     4741 tcagcgcgac accgggcgta ttgcgctgga gtgggcgcag catcgacgcc gatggggtat
     4801 cgatccgcgc caccaaatgg ggttcaggct gacgcgtcgt ccgacggggc cgcttccgca
     4861 tcggcggcag tggcggcggc gtcgtccttg tcgtcatcgt cgtcgtcctt gtcgtcgcca
     4921 ccagggatga tgtccttgat cttgtcggtg acggcgctga ccttgtcctt gaccgaatcg
     4981 gggatcttgt cctcgatctt gtcgacgatg tcgccggcct tgtccttgac ggccgagaat
     5041 ttctccttga ccgagtcggg gatcttgtcc tcgatcttgt cgacgacgtc ttccgccttg
     5101 tccttgatgt cttcggccaa gtccttggcc ttatccatga atccgctcat gatggaacgt
     5161 taccggtcag cccgaccggg cacgacacgc ccgtaccgcc caagccgcag tagccgttgg
     5221 ggttcttgcc caggtactgc tggtggtacg gctcggcgta gtagaagtgg tcgagcggag
     5281 cgatctcggt ggtgatcgtg tggtaaccct tggcgtcgag cgcggcctgg tacatcgcct
     5341 ggcttgcttg ggcctgggcg gcctgtgcgg gcgaggtggt gtagatcgcg ctgcgatact
     5401 gcgtgccgac gtcgttgccc tggcggtagc cctgcgtggg gtcgtgcgac tcccagaaca
     5461 acttgacgag ctcctcgtag ctgacggctg tcgggtcgaa cacgacctgg accacctcgg
     5521 tgtggccggt gcgcccggag cagacctctt cgtaggtcgg gttgggcgtg tagccaccgg
     5581 cgtaaccgga tgccgtggag tacacgccgg cggtctgcca gaacttgcgc tcggcacccc
     5641 agaagcaacc cagcccgaac accgctgtct ggtacccgtc gggccacggc ccgaccagcg
     5701 gatgcccggt gacgaagtgg gcgtcgggca ccggcatcgt ctggtcggtg cgcccgggca
     5761 gcgcctcgtc ggcagcgatc atctcagcct tcttgaagaa cacgatacac tcnnnnnnnn
     5821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnng agccgcacgt gaggagcggc caaaggcgcc
     5941 tccggcgccg gaggccagtc ctcaccgacc agaatgtccg aagccgccgc ctcggtcgtg
     6001 accgtcgagg gtcgtcgtcg attgccacac cacgtgctcg acccgctgca acaccaactg
     6061 ggcgatgcgg tcgccgcgct gcaccgtgta ggcctcggcc ggatcggtgt tgagcaggac
     6121 cactttgatc tcaccgcggt aggccgcgtc gatcagaccc ggcgcgttgg ccaccgtgac
     6181 cccgtgcttc aacgccagcc
//
LOCUS       DAORNF010000098         6189 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_9408_length_6189_cov_1.699217, whole genome shotgun sequence.
ACCESSION   DAORNF010000098 DAORNF010000000
VERSION     DAORNF010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 6189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6189
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9408_length_6189_cov_1.699217"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="PLV68_05430"
     CDS             <1..195
                     /locus_tag="PLV68_05430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00718.1"
                     /translation="LAAHGVPPRQRDPFAERAFPADRYGLSPAAWSDVDESLHEPGLI
                     WGAWKAKTVLARRRPPQASH"
     gene            complement(213..>1064)
                     /locus_tag="PLV68_05435"
     CDS             complement(213..>1064)
                     /locus_tag="PLV68_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00719.1"
                     /translation="PGVSVRRVVVPLQKGLVVRGAPPAGLGTVPARSTLAIVDAWLAE
                     ARAELGNGWALIQLEHAAPRLVCSLGTAAMHRELIEFGFAGAAAGQSLELTLHSVRLL
                     AAVVEPSTARLVDTREVAVMVSEGWNAAELARLQVANDLIPMPTFLHLLTQRCISRSG
                     DADIGPETNDVALVVIDVQAIEIDKVDLARMSESFVTAIRSVWGASQLLAQGANANLV
                     TMIDRDARLHGRTRELRRLIAGVQVPGDRRVHVWVEPLAVSVIHVRPHLESLVGPIDA
                     GPSLPVP"
     assembly_gap    1065..1146
                     /estimated_length=82
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1191..>1710
                     /locus_tag="PLV68_05440"
     CDS             1191..>1710
                     /locus_tag="PLV68_05440"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRE00720.1"
                     /translation="MTDTTLLRSVLSDAELILATRGGDGDAYGELYRRHLPAAQAAAR
                     ALTRSRADADDVVADAFSNVLGVLQRGMGPEVSFRPYLLTTVRNTFYERARRNQRDRP
                     IDSPDDSVDYALLDLAASDADRSLIAEAFASLPERWQLVLWHTEVEGRTAAEVAPMLG
                     IAANAVAALSYRA"
     assembly_gap    1711..1720
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1721..>2559
                     /locus_tag="PLV68_05445"
     CDS             <1721..>2559
                     /locus_tag="PLV68_05445"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HRE00721.1"
                     /translation="VHGCVVLFWGKGLREAYLQTHLQQPLDLDCRACVSNLGAYVRDG
                     LSARERRRVDEHLGQCTSCPPLLAELTETNTHLRAALIPLVVGVPAAKYLAGLGGAKG
                     LAATMRRMPRSQQAGVAAAAVAAIVGLAAIGIAAGRDDHRSIVAAEASPEPSTTLRVT
                     PTTSSATSSATSPASTDPSTDHTDPFSSDTTDPYATSVDPNAPYDSADIFVPTYPSVP
                     YVPNTQPPTTPPPPTPGTAPRKPTTTTTQQPTTTTNNVNNTYPTVPPVVDSTTTTSPA
                     TTT"
     assembly_gap    2560..2649
                     /estimated_length=90
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2650..3964
                     /locus_tag="PLV68_05450"
     CDS             <2650..3964
                     /locus_tag="PLV68_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00722.1"
                     /translation="NGSTIIRVNARNGEPGGGGSHRRPMALTEASNVLVTIPNLPMGV
                     TFGGVDQAAWACALTTDAAGTTKTLQCSLPDLAEGAATTADITLNLDATVTALALTPT
                     ISADGADPYVADTPLSVPVTQIANSIGVFQDTGDLIVIGNASVTCDDVASATCADARD
                     ADLPLTANPSLNRQDQPMVLLGADAGAGVTNASSATLTLPQGATVAKAYLVWGGLSDV
                     MLPPTAQPAELAVLVGSATVSTGGLGATVLASNVARLEGNVYSAWKDVTSSFTSLSGT
                     VEITVSNLHLAATTASFGGWSLIVVTHDTTQPERLMVVSLPNASFSTPATKFTASIPF
                     AGSANSTEATVGIVALEGDRGRIGDYLDVGSGRVGNAFNGAVTGGRTPSYKNNFGFDL
                     TLHSTSITAGDTPETDRVVQIEAGVDGASGDLVRLIAITYAITLD"
     gene            complement(3982..4878)
                     /locus_tag="PLV68_05455"
     CDS             complement(3982..4878)
                     /locus_tag="PLV68_05455"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HRE00723.1"
                     /translation="MSTGDVHRYRVEHRTTYRYGSAMTDGYTVACLVPRPTDRQRVIE
                     SHVWLEPVPSEQDSYLDVFGNLVYQFGLHEPHRQVTVAASSVVDLVELGQPTDDTPWE
                     CAVEMLDAATDDLAIETGFFRSASTFVDLVGLGDALRAIARRSFGAGRLIVDAAAALC
                     TDIHDTFRFDAGFSDVSTPLHRVLAARRGVCQDFAHLAVGALRSIGLAARYVSGYIET
                     DAAPGAERLVGADASHAWCSLWTPGAGWVDFDPTNAHLPVNRHITTAWGRDYADVTPV
                     RGVMIGPATTQALDVAVDVAPL"
     gene            complement(4865..>6189)
                     /locus_tag="PLV68_05460"
     CDS             complement(4865..>6189)
                     /locus_tag="PLV68_05460"
                     /inference="COORDINATES: protein motif:HMM:NF016086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HRE00724.1"
                     /translation="LTRLADLGAPLARVPMATPHAAVTGTSQAEAQVVIRLFAVDDGT
                     TVSVLAGGTGRVLAEGDDPRLPTACVAKDVWVVGGTVAPVIVGPRLPQVDFGGSLPTR
                     AADALYWTNRSAERAEAMARTVGAVVRRLEQDPGLAVLDEGAWARRMIAVLRAIRHNA
                     GVPAEPGSANPLPPDAELGLSAHGELTAELGAVPAAIAHEIGALLTEATTVREYLSTA
                     TGRVLADLAELRAAISSGSAGIHDLFPVLGDFAAFAGLWHESIVRGPAWRIGDTGRRL
                     ERALVVLDLVEAAVAGMGVDAMSAPAACAGSGVADDVYAAEIEAGVIEVLLSANESLV
                     AYRRRYRSDVEFDATIALLIHDATNPRSLVSAIDLLERHAVDGDWQVGVELAVRARQA
                     LTLPLAELLPATRAVIEEIGLRVVERWFSSPVAPILMRPTDLVCHEHG"
BASE COUNT          992 a         2245 c         1932 g          838 t
ORIGIN      
        1 ctcgccgcgc acggcgtgcc accgcgccag cgcgatccct tcgccgagcg ggccttccca
       61 gccgaccgct acgggctcag cccggccgcg tggtcggacg tcgacgagtc gttgcacgaa
      121 cccggcttga tctggggcgc ctggaaggcc aagaccgtgc tggcccgccg gcggccaccg
      181 caggcgtcac actgacgcgt cgctccgggg cgtcacggca cgggcagcga cgggcccgcg
      241 tcgatcgggc cgaccaggct ctcgaggtgg ggacggacgt ggatgaccga cacggcgagc
      301 ggttcgaccc acacgtgcac ccggcgatca ccgggcacct gcacgccggc tatcagccgg
      361 cgcagctcgc gggtgcggcc gtgcagccgg gcgtcgcgat cgatcatggt gacgaggttg
      421 gcgttggcgc cctgagccag tagttggctc gccccccaca cgctgcggat cgcggtcacg
      481 aacgactcgc tcatccgcgc caggtcgacc ttgtcgatct cgatcgcctg cacgtcgatc
      541 accaccagcg cgacatcgtt ggtctcggga ccgatgtcag cgtcgccgct gcggctgatg
      601 cagcgctggg tgagcagatg gaggaaggtg ggcatcggga tgagatcgtt ggcgacctgc
      661 aaccgggcga gttccgccgc gttccaacct tcactcacca tcactgcgac ctcccgcgtg
      721 tcaacgagcc gtgctgtcga gggctcgacc accgccgcca gcaatcgcac gctgtgcagg
      781 gtgagctcca gtgattgccc agcggcggcc ccggcgaaac cgaattcgat cagttcccga
      841 tgcatcgccg ccgtaccgag ggagcacacg agccggggtg cagcgtgctc gagttggatc
      901 aacgcccatc cgttgcccaa ctcggcgcgg gcctccgcga gccacgcatc gacgatcgcc
      961 agggtggaac gagcggggac ggtgcccaga ccggctggcg gcgcccccct cacgaccaac
     1021 cctttctgca acggaacgac aacacgacga acagacacac caggnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnccga agcacggcaa acgatccgga actaccctgt acctcgcgtt atgaccgata
     1201 ccaccctgtt gcgctccgtc ctcagcgacg ccgaactcat cctcgccacg cgcgggggcg
     1261 acggcgacgc gtacggcgag ctctaccgcc gtcacctccc tgccgcgcag gcagcggcgc
     1321 gggcgctgac gcgatcacgg gccgacgccg acgacgtggt ggccgacgcg ttctccaacg
     1381 tgttgggtgt gctccagcgc ggcatgggcc ccgaggtcag cttccggccg tatctgctca
     1441 ccacggttcg caacaccttc tacgagcggg ctcgccgcaa ccagcgtgat cggcccatcg
     1501 actcgcccga cgactccgtc gactacgcgc tgctcgactt ggccgcctcc gacgccgatc
     1561 gcagcctgat cgccgaggca ttcgccagct tgcccgagcg gtggcagctg gtgctgtggc
     1621 acaccgaggt cgaaggccgc accgcggccg aggtcgcgcc gatgctgggc atcgccgcca
     1681 acgccgtcgc cgccctgtcg taccgggccc nnnnnnnnnn aggtccacgg ctgcgtcgtg
     1741 ctgttctggg gcaaaggcct gcgcgaggcc tacctgcaaa cacacctgca gcaaccgctc
     1801 gatctcgatt gccgcgcctg cgtgtccaac ctcggtgcct acgtgcgcga cggcctgtcg
     1861 gcgcgcgaac ggcgacgggt cgacgagcat ctcgggcagt gcaccagctg cccgccgctg
     1921 ctggccgaac tgaccgagac caacacccac ctgcgcgctg cgctgatccc gttggtggtc
     1981 ggcgtgcccg ccgccaaata cctcgccggc ctgggcgggg ccaagggcct ggctgccacg
     2041 atgcgacgca tgccccgctc gcagcaggcc ggtgtcgccg cggccgctgt cgccgccatc
     2101 gtcgggttgg ccgcgatcgg catcgctgct gggcgcgacg accatcgcag catcgtcgcg
     2161 gccgaagctt cgccggagcc gtcgaccacg ttgcgggtta cgccaaccac gtcgtcggca
     2221 acgtcgtcgg ccaccagccc ggccagcacc gacccgtcca cggaccacac cgatccgttc
     2281 agcagcgaca ccaccgatcc ctacgccacc tccgtcgacc ccaacgcgcc gtacgacagc
     2341 gccgacatct tcgtgcccac ctacccctcg gtgccgtacg tgcccaacac gcagccgccc
     2401 accacgcctc ccccgccaac accgggcacc gcgccgcgca agcccaccac gaccaccacg
     2461 caacagccga ccaccaccac gaacaacgtc aacaacacct accccaccgt gcccccggtg
     2521 gtcgacagca cgacgacgac ctctccggcc accacgaccn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnc aacggcagca cgatcattcg tgtcaacgcc cgcaacggcg aacccggtgg
     2701 cggtggttca caccgtcgcc ccatggcgtt gaccgaagcc agcaacgtgc tggtcaccat
     2761 ccccaacctg cccatgggcg tcaccttcgg cggtgtcgac caggccgcct gggcctgcgc
     2821 gctcaccact gacgccgccg ggacgaccaa gacgctgcag tgctcgctcc ccgacctggc
     2881 cgagggtgcg gccacgactg ccgacatcac gttgaacctc gatgccacgg tcaccgcact
     2941 ggcgctgacc cccaccatct cggccgacgg ggccgatccg tacgtggccg atacgccgct
     3001 gtcggtgccg gtcacccaga ttgctaactc gatcggggtc tttcaagaca ccggcgacct
     3061 gatcgtgatc ggcaacgcct cggtcacctg cgacgacgtt gcgtcggcca cgtgtgccga
     3121 cgcccgcgac gctgacttgc cgctcaccgc caacccctcg ctcaaccgcc aggatcaacc
     3181 gatggtgctg ctcggggccg acgcgggcgc aggcgtcacc aacgcctcct cggcaacgct
     3241 cacgctgccc caaggcgcca ccgtggccaa ggcctacctg gtgtggggcg ggctcagcga
     3301 tgtgatgctg ccgcccacgg cgcagcctgc tgaactcgca gtcttggtgg gttctgcgac
     3361 cgtctcaacc ggcggcctgg gcgcaacggt gctggccagc aacgtcgcga ggctcgaagg
     3421 caacgtctac tcagcatgga aggacgtcac ctcatcattc acatcgctgt ccggcaccgt
     3481 tgagatcacg gtgtcgaacc tgcacctcgc cgcgaccacc gccagtttcg gtggatggtc
     3541 cttgatcgtg gtcacccacg acacgaccca gccggagcgg ttgatggtgg tgtcgctgcc
     3601 caacgcgtcg ttctcgaccc ccgccaccaa gttcacagcc agcattccct tcgccgggtc
     3661 ggccaactcc acggaggcga ccgtcggcat cgtcgccttg gaaggcgacc gcggtcgcat
     3721 cggcgactac ctcgacgtcg gctccggcag ggtcggcaat gcgttcaacg gcgctgtgac
     3781 aggcggccgc acgccgtcgt acaagaacaa tttcggcttc gacctgacgc tgcacagcac
     3841 ctcgatcacc gctggggaca cgcccgagac cgatcgcgtc gtccagatcg aagccggcgt
     3901 cgatggagcc tccggcgacc tcgtgcgcct gatcgcgatc acctacgcga tcacgctcga
     3961 ctgatcagtt cgacccagcc ctcacaaggg tgcgacgtcc acggcgacgt cgagcgcctg
     4021 ggtggttgcc ggcccgatca tgacgccgcg cacgggggtg acgtcggcgt agtcgcgccc
     4081 ccaggccgtg gtgatgtggc ggttcaccgg caggtgcgca ttggtgggat cgaagtcgac
     4141 ccaaccggcc cccggcgtcc acagcgagca ccaggcgtgc gatgcgtcgg caccgacgag
     4201 ccgctcggca ccgggggcgg cgtcggtctc gatgtacccg ctgacgtagc gcgccgccaa
     4261 cccgatcgac cgcagcgctc ccaccgccag atgggcgaag tcctggcaca cgccgcggcg
     4321 ggccgcgagc acccggtgca acggtgtcga cacgtcggag aacccggcat cgaagcggaa
     4381 cgtgtcgtgg atgtcggtgc acagcgcggc cgcagcgtcg acgatgagac gtccagcccc
     4441 gaacgagcgc cgcgcgatgg cgcgtagggc atcacccagg ccgaccagat cgacgaatgt
     4501 cgacgccgac cggaagaacc cggtctcgat cgccaagtcg tccgtggctg catccaacat
     4561 ctcgacggcg cactcccacg gggtgtcgtc ggtgggctgg ccgagctcga ccaggtcgac
     4621 cacgctgctg gccgcgaccg tcacctggcg atggggctcg tgcaggccga actggtacac
     4681 gaggttgccg aacacgtcga ggtacgagtc ctgctccgac ggcaccggtt ccagccacac
     4741 atgcgactcg atcacccgct ggcgatcggt gggacggggg accaggcagg cgaccgtgta
     4801 cccgtcggtc atcgccgacc cgtagcgata cgtggtgcga tgctcgaccc ggtagcggtg
     4861 cacgtcaccc gtgctcatgg cagaccagat cggtcggtcg catcaggatc ggagcgaccg
     4921 gcgacgagaa ccaacgctcg acgacccgca gcccgatctc ttcgatcacc gctcgggtcg
     4981 cgggcaacaa ctcggccagc ggcaacgtca gcgcctgacg cgcccgaacg gcgagctcga
     5041 ccccgacctg ccaatcgcca tcgaccgcgt gacgttcgag gagatcgatc gccgatacca
     5101 gcgagcgcgg gttcgtggcg tcgtggatca gcagggcgat ggtggcgtcg aactcgacgt
     5161 cgctgcgata ccgccggcgg taggcgacga ggctctcgtt ggcactcagc aacacctcga
     5221 tcacccccgc ttcgatctcg gccgcgtaca cgtcatcggc aacacccgac ccggcgcatg
     5281 ccgcgggggc cgacatcgcg tcgacaccca tcccggccac ggcggcttcg accagatcga
     5341 gcaccaccag ggcccgctcc agtcggcggc ccgtgtcgcc gatgcgccac gcgggcccac
     5401 gcacgatgct ctcgtgccac aacccggcaa acgcggcgaa gtcgccgagc accgggaaca
     5461 ggtcgtggat cccggccgaa cccgaactga tcgccgcgcg caactccgcc agatcggcga
     5521 gcacgcgacc tgtcgcggtc gacaggtact cgcgcacggt cgtcgcctcg gtgagcaacg
     5581 ccccgatctc gtgggcgatg gccgcgggca ccgcgccgag ctcggcggtg agctcgccgt
     5641 gggcgctgag gcccagttcg gcatcgggcg ggagcgggtt ggccgaacca ggttcggccg
     5701 gaacgcctgc gttgtggcga attgcccgca gcacggcgat cattcgccgc gcccaggcac
     5761 cctcgtccag tacggccaga ccggggtctt gttcgagccg tcgcacgacc gctccgaccg
     5821 tgcgggccat cgcctcggcc cgttcggcgg aacgattggt ccaatacaac gcatcggcgg
     5881 ctcgcgtggg gagcgagccg ccgaagtcga cctggggtag gcggggacca acgatcactg
     5941 gggcaaccgt gccgcccacg acccacacgt ccttggcgac gcacgcggtc ggcagacgcg
     6001 ggtcgtcgcc ttcagccaac acccgccccg tgccgcccgc gagcaccgac accgtggtgc
     6061 cgtcgtcgac cgcgaacagg cggatgacca cttgcgcctc ggcctgcgag gtgccggtga
     6121 ccgccgcgtg cggtgttgcc atcggcaccc gcgccagcgg cgcgcccagg tcggcgagcc
     6181 gagtcaacg
//
LOCUS       DAORNF010000099         6182 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_9416_length_6182_cov_1.422719, whole genome shotgun sequence.
ACCESSION   DAORNF010000099 DAORNF010000000
VERSION     DAORNF010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 6182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6182
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9416_length_6182_cov_1.422719"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            547..>1232
                     /locus_tag="PLV68_05465"
     CDS             547..>1232
                     /locus_tag="PLV68_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00725.1"
                     /translation="MSEHQLPADVTDGPASEVVASLPLKPTTLDVLSVDDEVVLITKA
                     KVVRVGVESDGTTRTITMKLKPSGTFPLPQEEGRERYARAASEHRVGVAERSGEYPLP
                     GYGDVGYTDGSGVVLTDAEVAERQGRGLPVVEVIPAVVVYSDRSRVMWPDDFASGAVR
                     PQIGDALNEDEPEVMVEDLLDAKTGESLIDAEAAPGEPGDEVVILVDDDEDVAVDEVV
                     DDPDPGLVSF"
     assembly_gap    1233..1253
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1254..1793)
                     /locus_tag="PLV68_05470"
     CDS             complement(<1254..1793)
                     /locus_tag="PLV68_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00726.1"
                     /translation="MRAAMVRQHPFRFDGPLGQGAAWSEPTLRVVHRSVEQSDCYEVP
                     FALFGERTDGRLLLGLLGVVLQLHLQVRVDQLVLDGFNAVTDHGDNRPADQGCERSQV
                     VLDSAGVLLAVVEGRGGIAQALIGRPNRRVVGGVHHSTSNRATIASTTLDRGFAAASS
                     NTIARRNRSADSWTRTRNFS"
     gene            1847..2158
                     /locus_tag="PLV68_05475"
     CDS             1847..2158
                     /locus_tag="PLV68_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00727.1"
                     /translation="MWAIVEIMGRRTRAGMCSDASIGGAQLLRVEHPTRRDHTGEEPV
                     VEYYAPQAIFAIRPCSQDEATQVAAWAWPERPSTPALSGPAADLVDTEDDDDDDDEVP
                     F"
     gene            2211..>2464
                     /locus_tag="PLV68_05480"
     CDS             2211..>2464
                     /locus_tag="PLV68_05480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00728.1"
                     /translation="MAAKKAPATRIQNYGKGHGYFLDGEKVRGVTTIIGDGIPKPALL
                     DWSAKMTAQFVVNRLKTAKRSDGTTAYIADDVIADALAWNR"
     assembly_gap    2465..2566
                     /estimated_length=102
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2567..3128
                     /locus_tag="PLV68_05485"
     CDS             <2567..3128
                     /locus_tag="PLV68_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00729.1"
                     /translation="EVHKLAERLARGEEIDVPELLAGHVQSYLRFLEEWDPQRALLEF
                     VCINRRWRDMGKGDMIAYFEALPEWIAEMIGKDDGWGLLDIKTSRSGIFAETALQTAA
                     YRNAETMIVGVEDGVAVEEPMPPIDFTGAIWCRHDGYSLYAFRNHPHTFRTFLYAAQV
                     GEFMDWKDGPAASIRSAELPAPSVVS"
     gene            3400..3807
                     /locus_tag="PLV68_05490"
     CDS             3400..3807
                     /locus_tag="PLV68_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00730.1"
                     /translation="MSEPVNVIVLCEVSHNGQPLRDGKRIVLGRADGKPLGWVRYLDQ
                     GTWWKNAAGEWIEVPSIDARYAGNIIGWLERRAQQLHWLYCLALCDMGGPQGDMASLA
                     WDDELDDVSRADPLPWLQSTALYRAVAARAEAS"
     gene            3938..5248
                     /locus_tag="PLV68_05495"
     CDS             3938..5248
                     /locus_tag="PLV68_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00731.1"
                     /translation="MSDIPGTGLALYTPHQQVELSKGVWTYAEAIASTDFVPKALRGN
                     PPAVMACMMKGTEVGLPLMASLSAIHVIEGRPTMSAESMRAVVIARGHKIWVEESSSV
                     RCTVGGRRQGDDRDSRFTFTIDDARAAGLLGKEVWKRYAAAMLLARATAMLCRAVFPD
                     CLAGLSYTREELEAGFAPDDYGTDIVDAVAVEAEQPPKRTAQAKRTATRGAARTTPDA
                     PVEDRTASQQRPPAPPLPGEEDDIIDADVIEDDLPGRDHEPEPVQDPEPYEQGDGPRM
                     TGPQMIAMLLTRHGVTARADKIRAVHQIIGRPIESTKDLTMDETSMILAVLNALGDGE
                     LFPFDDEPVAESEAEAVELPLEDAPAPAASATKDRRAAVSDPEDWDADRWRQFIQARK
                     KKPTQVMPEFRRIASESGVTIATLDDIAGSGVAGMLVGWLEDNA"
     assembly_gap    5329..5451
                     /estimated_length=123
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5452..>5689
                     /locus_tag="PLV68_05500"
     CDS             <5452..>5689
                     /locus_tag="PLV68_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00732.1"
                     /translation="GYDPQHWQHVAAVVRRAQAEDRGIYESLTVEFGASKSTVRNWVF
                     KARRLGLLDDIAKTPSSAVRAATAGRPIRHGDDHW"
     assembly_gap    5690..5945
                     /estimated_length=256
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          947 a         1880 c         1976 g          877 t
ORIGIN      
        1 caccccgccc ctacgaccag gaccgggacg acgtcgcgtt cgatgctgac ccgacgcctg
       61 ctggtggcgt gcgacggcga gagccgacac acaactggtg gatgccatga tccgccgggc
      121 ctggtactac gcgcgtgcct ggtgggccgg tgtcagggcg cgggtccacc cgtcgttcac
      181 gtgcagcgcg cctggctgcc accgcgaagc gtcctacatc gtcgccggcc aatggttctg
      241 catcgcgcac gccgaccaac ggagccgccg atgaacgttg gcccccagat cctgtccgtg
      301 cggcccgttc gccatcgggc ccggcaggat cgctcgcaca tccgggtgcg agcccgacct
      361 gccgccgcca ccccagcgcg gcaggtcgag atctcccacg gctggccagt ccggtcggcc
      421 ggccacggct cgacgagagc cgcgccgccc taagtcgcca caccgcgacc agctgcaaca
      481 ccgtgccctt cccccgtccg ggggcgtggt caacgcgtcc ccgaaaccac ccaccgagag
      541 gaatccatgt ccgaacatca actacccgct gacgtcactg atggccccgc ttccgaggtc
      601 gtcgcgtcgc tcccgctgaa gccgaccacg ctcgacgtgc tcagcgttga cgacgaggtc
      661 gtgctgatca ccaaggccaa ggtggtcagg gtcggcgtcg agtccgacgg caccacccgc
      721 acgatcacca tgaagctgaa gccgtccggg acgttcccac tcccgcaaga ggaaggtcgg
      781 gaacggtacg cgcgggccgc gtcggagcat cgggtcggtg tcgccgaaag gtccggtgag
      841 tacccgttgc ccggctacgg cgatgtcggc tacaccgacg ggtccggtgt cgtgctcacc
      901 gacgccgagg tcgctgagcg gcagggtcgc gggctgccgg tggtcgaggt gatccccgct
      961 gtcgtggtgt attcggatcg gtcgcgggtg atgtggccgg acgacttcgc gtccggtgct
     1021 gttcgtccgc agatcggtga cgcgttgaac gaggacgagc cggaggtgat ggtcgaggac
     1081 ttgttggacg cgaagacggg cgagtcgctc atcgatgccg aggccgcgcc gggcgagccc
     1141 ggtgacgagg tggtgatcct ggttgacgac gacgaggacg tcgcggtcga cgaggtcgtt
     1201 gatgatcctg atccggggtt ggtgtcgttc ttnnnnnnnn nnnnnnnnnn nnngctgaag
     1261 ttgcgtgttc gtgtccagct gtcggcggac ctgttgcgtc gggcgatggt gttggaggaa
     1321 gcggccgcga agccgcggtc gagcgttgtg gaagcgatcg ttgcccggtt ggaggtgctg
     1381 tgatggacgc cacctaccac ccggcggttc ggtcggccga tgagtgcctg cgcgatgccg
     1441 ccacggccct caacaacggc aagcagcacg cccgcgctgt ccagcacaac ctgcgatcgc
     1501 tcgcagccct gatcggctgg ccggttgtct ccgtgatccg tgacggcgtt gaagccgtcg
     1561 aggaccagtt ggtcgacgcg tacctggaga tgcagttgga gcaccacgcc gagcaggccc
     1621 aaaagcaggc ggccatcggt gcgttcgccg aagagcgcga acggcacctc gtaacaatca
     1681 gactgctcaa cggagcggtg aacgacgcga agcgtcggct ccgaccaggc cgcaccctgc
     1741 ccgagtgggc cgtcgaagcg gaacgggtgc tgcctgacca tcgccgcgcg caaggtcttg
     1801 tccttcccgg cgtagtcggt gcagatccag tggaggcgcc gttctgatgt gggccatcgt
     1861 cgagatcatg gggcgtcgca cccgtgccgg gatgtgctcg gacgcgtcga tcggtggcgc
     1921 gcagctgctc cgcgtcgagc acccgacacg ccgcgatcac accggcgagg aaccggtcgt
     1981 cgagtactac gcgccgcagg ccatcttcgc gatccggccg tgctcgcagg atgaagcgac
     2041 gcaagtcgcg gcgtgggcgt ggcccgaacg gccgtcaacg cccgcgctca gcgggcccgc
     2101 cgccgacctc gtggacaccg aagacgacga tgacgacgac gacgaggtcc cgttctgatg
     2161 ggcgacggtg cacacctcgg agcccgcgac ctcttgatcg ggggcgagtg atggctgcga
     2221 agaaggctcc cgcgacgcgg atccagaact acggcaaagg ccacgggtac tttcttgacg
     2281 gcgagaaggt ccgcggcgtc accaccatca tcggcgacgg catccccaag cccgcgctcc
     2341 tcgactggtc cgcgaagatg accgcccagt tcgtcgtcaa ccggctcaag accgccaagc
     2401 ggagcgacgg caccaccgcc tacatcgccg acgacgtcat cgccgacgcg ctcgcttgga
     2461 accgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncgag gtccacaagc
     2581 tcgccgagcg actggcccgc ggtgaggaga tcgacgttcc cgaactgctg gccggtcacg
     2641 tgcagtcgta tctgcggttc ttggaggagt gggaccctca gcgggcgctg ctcgagttcg
     2701 tgtgcatcaa ccgccgctgg cgggacatgg ggaagggcga catgatcgcc tacttcgagg
     2761 ccctgcccga gtggatcgcc gagatgatcg gcaaagacga cgggtggggg ctgctcgaca
     2821 tcaagacgtc gcggtcgggg atcttcgccg agacggcgtt gcagaccgcc gcgtatcgga
     2881 acgccgagac gatgatcgtc ggtgtcgagg acggggtcgc ggttgaggag ccgatgcccc
     2941 cgatcgactt cacgggcgcg atctggtgcc gccacgatgg ctattcgctc tacgcgttcc
     3001 gcaaccaccc acacacgttc cggacgttcc tctatgcagc gcaggtcggc gagttcatgg
     3061 attggaagga cggtccagca gcgtcgatcc ggtcggctga actgccagcc ccgtccgtgg
     3121 tgtcgtgatg cgcgcccttg acgtcgcgaa cgctgcgctg atggaagtcg gcctgccacc
     3181 gaccgcacgc cggatgccga atggcacgaa gcgcatcgcc gggccgcggg ccatgacccg
     3241 gcgggccgtc gccctcggcc tgatcgccga gagtggacct gacgcgctga tgacctgcct
     3301 cgcctgcatc ccgctagcga atgctgccgg tgccagtacg aactgcgtca ctgctggtga
     3361 cgttctccgt ggccgcacct gctcgaaggg ggatcaacca tgagcgagcc ggtcaacgtc
     3421 atcgttctct gcgaggtcag ccacaacggg cagccgttgc gtgatgggaa acggattgtg
     3481 ctggggcgcg ctgacggcaa gccgctcggc tgggtccggt acttggacca aggcacctgg
     3541 tggaagaacg ccgccggcga gtggatcgag gtcccgtcga tcgacgcaag atatgccggg
     3601 aacatcatcg gctggcttga acgtcgcgcc cagcagttgc actggctgta ctgcctggcg
     3661 ctctgcgaca tgggcggccc gcagggcgac atggcgtcgc tcgcgtggga tgacgagctt
     3721 gacgacgtgt cccgagcgga cccgttgccg tggctgcaga gcactgcgct gtaccgggcg
     3781 gtcgccgccc gcgctgaggc ctcctagctg tcccggcata cgccggtcgc ccgcgtcctg
     3841 cccggactgt ggcggacctc tcacccgtgg ttgtgggccc gggtgagagg tcgacaacca
     3901 acgaccccca cacccaacga tcgagaagga gcacagagtg agcgacattc caggaacagg
     3961 gctcgcgctc tacaccccgc atcagcaagt cgagctctcc aagggcgtat ggacctacgc
     4021 ggaggcgatc gcgtccaccg acttcgtgcc gaaggcgttg cgcggcaacc cgcccgccgt
     4081 catggcgtgc atgatgaaag gcactgaggt tgggctaccg ctgatggcca gcttgtcggc
     4141 gatccacgtg atcgagggac gcccgacgat gtcggccgag tcgatgcgag cggtcgtgat
     4201 cgctcgcggg cacaagatct gggtggaaga gtcgtcgagc gtgcggtgca cggttggtgg
     4261 ccgtcgccag ggggacgatc gggactcgcg gttcacgttc acgatcgacg acgcccgcgc
     4321 cgccgggttg ctgggcaagg aagtgtggaa gcggtacgcg gccgcgatgc tgctcgcccg
     4381 cgccaccgcg atgctgtgcc gcgcagtctt cccggactgc ctcgctggcc tgtcgtacac
     4441 gcgggaggag ctcgaggccg ggttcgcgcc ggacgactac gggaccgaca tcgtggacgc
     4501 ggtagcggtt gaggctgagc agccaccgaa gcggactgcg caggcgaaaa ggacggcgac
     4561 gcggggtgcg gcgcgcacca ccccggacgc tccggtcgag gatcgcacgg cgtcgcagca
     4621 gcggccgcct gcacctccgc tccccggcga agaggacgac atcatcgacg ctgacgtgat
     4681 cgaggacgac cttcccggac gcgaccacga acccgagccc gtgcaggacc ctgagcctta
     4741 cgagcagggc gacggtccgc ggatgaccgg gccgcagatg atcgcgatgc tcctcacccg
     4801 acatggcgtc acggcccgtg ccgacaagat cagggccgtc caccagatca tcggccgacc
     4861 gatcgagtcg acgaaggacc tgacgatgga cgagacgtcg atgatcctcg ctgtcctcaa
     4921 cgcgctcggt gacggtgagt tgttcccgtt cgacgacgag cccgtggccg aatccgaggc
     4981 cgaggctgtc gagctcccgc tggaagatgc ccctgcgcca gcagcgtcgg ccacgaagga
     5041 tcgtcgcgcc gctgtgtcgg acccggagga ttgggacgcg gaccggtggc gccagttcat
     5101 ccaggcgcgg aagaaaaagc cgacgcaggt gatgcccgag ttccggcgga tcgcgtccga
     5161 gtccggggtg acgatcgcga cgttggacga catcgccggg tcaggtgtcg ctgggatgct
     5221 ggtcggctgg ttggaggaca acgcgtgagc gtcgcggtcg gcgacacatt caccgctgat
     5281 gcgggacgcc ggttcacggt ggccgcgttg gatgtggatg gcgtatcann nnnnnnnnnn
     5341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nggctacgac
     5461 ccgcagcact ggcagcacgt cgccgccgtg gtgcgtcgag cccaggccga ggaccgaggc
     5521 atctacgagt cgctcaccgt cgagttcggt gcgtcgaagt cgacggtacg gaactgggtg
     5581 ttcaaggcgc ggcggctcgg cctgctcgat gacatcgcca agacaccgag cagcgccgtc
     5641 cgggctgcaa cggccggtcg acccatccgc cacggtgacg atcactggcn nnnnnnnnnn
     5701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5941 nnnnncacgc agcgtgttcc agcggtccca gcaccggatc tcgccgtcac cgacgcgtca
     6001 gccgacccga tggctgcgct cacccgctgc cgcatgaccc tcgcgaatgt cgccgccgag
     6061 tacctcgccg cgctcagggc cgaccagcgt ccgatcgaga agatcgcgac ccggcatcgc
     6121 gtgcaccaca aggtcgccaa caagtgggtc gccgctgccc gacgtgaggg ccttctcccg
     6181 cc
//
LOCUS       DAORNF010000100         6179 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_9421_length_6179_cov_1.716525, whole genome shotgun sequence.
ACCESSION   DAORNF010000100 DAORNF010000000
VERSION     DAORNF010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 6179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6179
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9421_length_6179_cov_1.716525"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            96..>357
                     /locus_tag="PLV68_05505"
     CDS             96..>357
                     /locus_tag="PLV68_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00733.1"
                     /translation="MTSPIDTSLLEPDDVVPNADQDPVLDNLVWHALTTTHARLAEGA
                     GFARRYRPDVAGFVAIESRTPQAWSALAALVGPGVDVVLSGGH"
     assembly_gap    358..440
                     /estimated_length=83
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <441..848
                     /locus_tag="PLV68_05510"
     CDS             <441..848
                     /locus_tag="PLV68_05510"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRE00734.1"
                     /translation="VAIERLTAADVTEMLALVQLTEPGPFRSGTIELGDYFGIRHGGR
                     LVAMAGERLQTPDYTEVSAVCTHPDVRGRGYASALTRRVAEGIRARGQTPILHVAEHN
                     LAAKAVYERLGFAVRTELHFIAVVSPVPPVAGA"
     gene            848..>1994
                     /locus_tag="PLV68_05515"
     CDS             848..>1994
                     /locus_tag="PLV68_05515"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HRE00735.1"
                     /translation="MGARPFHLSVALDGTGFHPASWRDPSARPTELFGPDYWIELGRT
                     AERGLLDFVTIEDSFGLQSSRSDGADGRVDQVRGRLDAVQIASLIGPVTRHVGLVPTA
                     ITTHTEPFHLASALSTLDYVSRGRGGWRPQVSVRQSDADHVGRRSVPKFNPDRIDDPQ
                     AHALIVDLFDEATDSVDVVRRLWDSWEDDAIIKHRETGRYIDRDKLHYVDFAGRFFSV
                     KGPSIVPRPPQGQPVVAALAHSQVPFEFAARAADIVFVTPADRAAVPEWIAQVRGAET
                     VVGRSGRPLLIHADLVVVLDADHATAERRKHRLDAWDGAPLRTDAAVFVGTPEELTNE
                     LTAWHALGLDGFRLRPAVIGHDLDEIVDEVVPALQRRGLFRTAYEPGL"
     assembly_gap    1995..2079
                     /estimated_length=85
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2081..>2470
                     /locus_tag="PLV68_05520"
     CDS             2081..>2470
                     /locus_tag="PLV68_05520"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HRE00736.1"
                     /translation="MSGAPRKQVILAAYFPGVNNTTVWHDPRSKSQIDFDSFIHLAQT
                     AERGKLDFFFLAEGLRLREQNGKIHDLDTVGRPDTLTVLTALAAVTRHLGLAGTLNTT
                     FHEPYELARQLATLDHLSGGRAAWNVVT"
     assembly_gap    2471..2501
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2515..>2748
                     /locus_tag="PLV68_05525"
     CDS             2515..>2748
                     /locus_tag="PLV68_05525"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HRE00737.1"
                     /translation="MDHADRYERAGEFVDVARELWDSWAADEIVADKELGRFLRDGPP
                     GAFEHRGPQFDIRGHFNVPRSPQGHPVVLQAGDS"
     assembly_gap    2749..2799
                     /estimated_length=51
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2800..>3133
                     /locus_tag="PLV68_05530"
     CDS             <2800..>3133
                     /locus_tag="PLV68_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HRE00738.1"
                     /translation="QLPGARVFYDDVKSRLAKYGRRRDELKILPAVTFVLGDTQADAE
                     EEARIVLRQQFSPQTSLLLLEQLWNRDLSAYDAEGPLPDVDPDVSATSIIQGRARMFS
                     DPLATAAE"
     assembly_gap    3134..3187
                     /estimated_length=54
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3188..3475
                     /locus_tag="PLV68_05535"
     CDS             <3188..3475
                     /locus_tag="PLV68_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00739.1"
                     /translation="IIHQMGQLTFVGTPASVAAQLDDFVQTNGSDGFVLAPHLTPTGL
                     DRFVDDVVPLLQERGVLRREYESTTLRGHLGLPDAGLIRAQRRQTLHAATA"
     gene            3726..>4271
                     /locus_tag="PLV68_05540"
     CDS             3726..>4271
                     /locus_tag="PLV68_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath family protein"
                     /protein_id="HRE00740.1"
                     /translation="MYEGEVIVAATYLTPGVYVEEVSSSSATLSAGATAVAAFVGFTA
                     KAPTDDPNDPEGVKPRLVTNWTQFENLYGGFAPGIMLPHSVYGYFNNGGSLAYIVRIP
                     NSTPATVSGTRALPSADRALGPALEFTTVEPNAPISVSVVPEAPADDAGDDDAPTFTI
                     EIVESGKVVESFPNLTLGKSDR"
     assembly_gap    4272..4410
                     /estimated_length=139
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4411..5334
                     /locus_tag="PLV68_05545"
     CDS             <4411..5334
                     /locus_tag="PLV68_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HRE00741.1"
                     /translation="EPASVSGRSFTGSEPSRTGINGLVIADDVTMVMIPDLITAATKD
                     DGSVDLNMWKSVQLTLINHCEGQANRMAVLDAPPGMSPQQVKEWREGVAMYDSQFAAF
                     YYPWVKVANPAATNGDTEILVPPSGHMAGIWARTDDTRGVWKAPANEVVRGALDVEID
                     ITKAEQGLLNPMGINCIRPFGTRGIRVWGARTLSSNTDWTYINVRRLFNMIETTIMNG
                     TQFAVFEPNDQTLWEGLKRTVGSFLRGLWRDGALFGATAEQAFYVKCDAETNPPESID
                     LGRVVVEVGIAPVKPAEFVIFRIAQIKDSSN"
     gene            5354..5806
                     /locus_tag="PLV68_05550"
     CDS             5354..5806
                     /locus_tag="PLV68_05550"
                     /inference="COORDINATES: protein motif:HMM:TIGR02241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HRE00742.1"
                     /translation="MPVTSPGEVQDVELVGSWFFIKLDNGIEGFFASASGFAIEMEVI
                     ETTVANVDTRTQKRPGTAKYQDISLKRNLSPDKKFWEWAKSIRDGELGFRTNGSLVLY
                     DMSGAEQSRWTFTNVWPSKWSASDLDVGSDDLMVEEVTLAVEQLVREK"
     gene            5814..>6179
                     /locus_tag="PLV68_05555"
     CDS             5814..>6179
                     /locus_tag="PLV68_05555"
                     /inference="COORDINATES: protein motif:HMM:TIGR02241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HRE00743.1"
                     /translation="MPDATHDEDFQGSFFALEIDGLTLGWFTACSGLSLEFDVTSYKE
                     SNGKSVVSRKRKYSAVVLKRGFTTNTVIHDWFKEVVDAAKETPYKTASIVLYTRQQKE
                     CARFNLEACWPSKLSMSDVQ"
BASE COUNT          991 a         1958 c         1797 g          990 t
ORIGIN      
        1 gaatcagccg gtgttgcctg ccgcgacccc ctcggcaggc aacaccggcg ccgacctcca
       61 cacatcccac ccctcgacct caagcgagcc ctggcatgac ttctcccatc gacacatccc
      121 tgctcgaacc ggatgatgtc gtcccgaacg ccgaccaaga ccccgttctc gacaacctgg
      181 tctggcacgc gctgacgacg acgcatgccc gcctcgcgga aggcgcagga ttcgccaggc
      241 gctatcgacc cgacgtggcc ggattcgtcg cgatcgagtc gcgcaccccg caggcctggt
      301 cggcactcgc tgcactggtc ggaccagggg tcgacgtcgt gctgtctggc ggccacgnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn gtggccatcg aacggctcac cgcggccgac gtgacggaga
      481 tgctcgcgct ggtgcaactc accgagcctg ggccgttccg gtcgggcacg atcgagttgg
      541 gcgactactt cggtatccgt cacggcggac gcttggtcgc gatggcgggc gagcggctgc
      601 aaacgcccga ctacaccgag gtcagtgcgg tgtgcacgca tcccgatgtc cgtggccgcg
      661 ggtatgcctc ggcgctgacc cgccgcgtcg ccgagggcat ccgtgctcgc ggtcaaaccc
      721 cgatcctgca cgttgccgag cacaacctgg ccgccaaggc ggtgtacgaa cggctcggct
      781 ttgcggtgcg taccgaactg cacttcatcg ccgtcgtatc tcccgtgcct cccgtggcgg
      841 gcgcgtgatg ggtgcgcgac cgttccacct gtccgtcgcc ctcgacggca ccggctttca
      901 ccccgcgtcc tggcgcgatc cgtcggcgcg cccgaccgag ctgttcggac cggactactg
      961 gatcgaactc gggcgcaccg ccgagcgcgg cttgctcgac ttcgtcacga tcgaagactc
     1021 cttcggcctg caatcatccc gctccgacgg tgccgatggg cgggtcgacc aggtgcgcgg
     1081 ccggctcgac gcggtacaga tcgcatcgtt gatcgggccg gtcacgcgcc atgtcggact
     1141 ggtgccgacc gcgatcacga cacacaccga gccattccat ctcgcgtcag cgctctcgac
     1201 gctcgactac gtcagccgtg gccgaggtgg ctggcgccca caggtgtcgg ttcgccagtc
     1261 cgacgcggat cacgtcggac ggcggagtgt gccgaagttc aacccagacc gcatcgatga
     1321 tccgcaggca cacgccttga tcgtcgacct cttcgacgaa gcgaccgatt ccgtcgacgt
     1381 cgtgcggcgg ttgtgggata gctgggagga cgacgcgatc atcaagcatc gcgagaccgg
     1441 gcgctacatc gaccgggaca agttgcacta cgtcgacttc gcaggccggt tcttcagcgt
     1501 caagggtccc tcgatcgtgc cccggccacc gcaagggcag ccggtcgtcg ccgcgttggc
     1561 gcattcgcag gttccgttcg agttcgcggc gcgcgccgcc gacatcgtgt tcgtcacgcc
     1621 agccgatcga gctgcggtgc cggagtggat cgcccaggtc cgtggggccg aaaccgtggt
     1681 tggtcgcagc ggtcgaccgc tgttgatcca cgccgatctc gtcgtcgtcc tcgacgccga
     1741 ccacgccaca gcagagcgtc gcaaacatcg gctcgacgcg tgggatggcg cgccgctgcg
     1801 cacggacgcg gccgtgttcg tcggtacacc cgaggagttg accaacgagc tgaccgcgtg
     1861 gcatgcgctg ggtcttgacg gcttccgcct ccgccccgct gtgatcggcc acgatctcga
     1921 cgagatcgtc gacgaggtcg tgccggcgct gcaacgccgc ggcctgttcc gcaccgcgta
     1981 cgaacccggc ctgtnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna atgagtggcg caccccgcaa
     2101 gcaggtcatc ctggccgcgt acttccctgg agtgaacaac accaccgtgt ggcacgatcc
     2161 gcggtcgaag agccagatcg acttcgactc gttcatccac ctcgcccaga ccgccgaacg
     2221 gggaaagctc gacttcttct ttctcgccga gggtctgcgt ctgcgcgagc agaacggcaa
     2281 gatccacgac ctcgacacgg ttgggcggcc ggacacgctg acggtgctga ccgcgctcgc
     2341 cgcggtcact cggcacctcg gcctggcggg caccttgaac acgacgtttc acgagccgta
     2401 cgagctggcc cgccagttgg cgacgctcga tcacctctcc gggggacggg cggcatggaa
     2461 cgtcgtcacg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngcgtggtgg cttcttggac
     2521 cacgccgacc gctacgaacg cgccggtgag ttcgtcgacg ttgctcgtga gttgtgggac
     2581 tcatgggccg cggacgagat cgtcgccgac aaggagcttg gccgcttctt gcgcgacggt
     2641 cctcccggcg cgttcgaaca tcgaggcccg cagttcgaca tccgaggtca cttcaacgtg
     2701 ccgcgcagcc cgcaggggca tccggtcgtg ctccaggcgg gtgacagcnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna tcaactcccc ggcgcacgcg
     2821 tgttctacga cgacgtgaag tcgcgtctgg ccaagtacgg gcggcggcga gacgaactga
     2881 agatcctgcc cgccgtgacg ttcgtgctcg gtgacactca agccgatgcc gaagaagagg
     2941 cgcggattgt gctgcgccag cagttcagcc cgcagacgtc gttgttgctg ttggaacaat
     3001 tgtggaatcg cgacctgtcc gcctacgacg cggaaggtcc gctgcccgac gtcgatcccg
     3061 acgtgtcggc cacctcgatc atccaggggc gggcacggat gttctccgac ccgttggcaa
     3121 cggccgccga atgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnatc atccaccaga tgggccagct gaccttcgta ggtacccctg cgagcgtcgc
     3241 cgcgcaactc gacgatttcg tccagacgaa cggttcggac gggttcgtgc ttgccccgca
     3301 cctgacgccc accggactcg accgctttgt cgacgacgtc gtgccgctgc tccaagagcg
     3361 gggcgtgttg cgccgtgagt acgagtccac gacgctgcgg ggccatctcg gcttgcctga
     3421 cgccgggctg atccgcgccc aacggaggca gacgttgcac gccgcgaccg cctgactgct
     3481 caccagcggt cactcggtgg gcgccgcttc gacgcacagt cctcggttga ccacgctgtg
     3541 gtgcgcgtcc ccctcgcaat tctccgtgac ccttgctaat gtgccctcga tcgcttcgtg
     3601 atccaaggac gggcagacat gccttggcga tgaagtgatc tctggctgcg ctcgagcggg
     3661 cgacgttctt ggcacgtcag agtcgtgttc gccgaccctc ggcgagcccg acgtgacaat
     3721 cgaacttgta cgaaggggaa gtgatcgtgg ccgctactta tctcacgcca ggcgtgtacg
     3781 tggaggaagt gtcgtcgtcg tcggcgacgc tgtcggcggg ggcgactgct gtcgccgcct
     3841 tcgtcggctt caccgccaag gctccaaccg acgacccgaa cgatccggaa ggtgtcaagc
     3901 cgcggctcgt caccaactgg acccagttcg aaaacctgta cggcgggttc gcccccggca
     3961 tcatgctgcc gcactccgtg tacggctact tcaacaacgg tggcagcctc gcctacatcg
     4021 tgcgcatccc caacagcacc ccggccacgg tcagcggcac ccgtgccctg ccctcggccg
     4081 accgtgctct cgggccggcg ttggagttca ccacggtcga gcccaacgcc cccatctcgg
     4141 tgtcggtcgt gccggaagca cccgcagacg atgccggcga cgacgacgcc cccaccttca
     4201 cgatcgagat cgtcgagtcg ggcaaagtcg tcgagtcgtt ccccaacctc accctcggta
     4261 agagcgatcg cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gagccggcct cggtcagcgg ccgttccttc
     4441 accgggtctg agccctctcg cacgggcatc aacggcttgg tcatcgccga cgacgtcacc
     4501 atggtgatga tccccgacct gatcaccgcg gccaccaagg acgatggcag cgtcgatctc
     4561 aacatgtgga agtcggtgca gctcacgctg atcaaccact gcgagggtca ggccaaccgc
     4621 atggccgtgc tcgacgcccc tcccggcatg tcgccccagc aggtcaagga gtggcgtgaa
     4681 ggcgtcgcca tgtacgacag ccagttcgcg gcgttctact acccgtgggt caaggtggcc
     4741 aaccccgccg ccaccaacgg cgacaccgag atcctcgtgc ccccgtccgg tcacatggcc
     4801 ggcatctggg cccgcaccga tgacacccgc ggtgtctgga aggcaccggc caacgaggtc
     4861 gtgcgcggcg cgctcgacgt cgagatcgac atcaccaagg ccgagcaagg cttgctcaac
     4921 ccgatgggca tcaactgcat tcgtcccttc ggcacgcgcg gcatccgcgt gtggggtgcg
     4981 cgcaccctgt cgtcgaacac cgactggacc tacatcaacg tccgccgcct gttcaacatg
     5041 atcgagacca cgatcatgaa cggcacgcag ttcgctgtgt tcgagccgaa cgaccagacg
     5101 ctgtgggaag gcctcaagcg tacggttggt tcgttcctgc gtggcttgtg gcgcgacggt
     5161 gccctgttcg gcgcaacggc cgagcaggcg ttctacgtca agtgcgacgc cgagaccaac
     5221 ccgcccgagt cgatcgatct gggccgtgtc gtggtcgaag tgggcatcgc cccggtcaag
     5281 cctgcggagt tcgtgatctt ccgtatcgcc cagatcaagg acagctcgaa ctgagcgata
     5341 ggaggacaac gacatgcctg tgacatcacc cggagaagtc caagacgtcg aactcgtcgg
     5401 ttcttggttt ttcatcaaac tcgacaacgg catcgaaggt ttctttgcca gcgcctcggg
     5461 ctttgcgatc gagatggagg tcatcgagac caccgtcgcc aacgtcgaca cccgtaccca
     5521 gaagcggccc ggcaccgcca agtatcaaga catcagcctc aagcggaacc tgtcgcccga
     5581 caagaagttc tgggaatggg ccaagtcgat ccgcgacggc gagctcggct tccgcaccaa
     5641 cggctcgctc gtgctctacg acatgagcgg cgcggagcag agccggtgga ccttcaccaa
     5701 cgtctggcca tcgaagtggt cggccagcga cctcgacgtg ggttccgacg acctgatggt
     5761 cgaagaggtc accctcgccg tcgaacaact cgtcagggag aagtgagaac gtcatgcccg
     5821 atgcaacaca tgatgaggat tttcagggtt cattctttgc cttggagatc gacggtttga
     5881 cgcttggctg gttcaccgca tgcagcggtc tcagcctcga gttcgacgtc accagctaca
     5941 aggagtcgaa cggtaagtcg gtcgtttccc gcaagcggaa gtacagcgcg gtcgtgctca
     6001 agcgtggatt caccaccaac acggtcatcc acgactggtt caaggaggtg gtcgacgcgg
     6061 ccaaggaaac gccgtacaag accgcttcga tcgtgctcta cacccgccag cagaaggaat
     6121 gcgcccggtt caacctcgaa gcgtgctggc cgtcgaagct gtcgatgtcc gacgtgcag
//
LOCUS       DAORNF010000101         6139 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_9507_length_6139_cov_1.736851, whole genome shotgun sequence.
ACCESSION   DAORNF010000101 DAORNF010000000
VERSION     DAORNF010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 6139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6139
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9507_length_6139_cov_1.736851"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1210
                     /locus_tag="PLV68_05560"
     CDS             <1..1210
                     /locus_tag="PLV68_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00744.1"
                     /translation="NAGLVSPVRPEPEPEPEPEPDPAVVNPSAPARYWDTRNEPTLDT
                     QFSNTGRVQAGATYTVQVSGRGDVPAGAKAVVANLVAIDPGTAGYATMFPCTVTPPVA
                     SHLNFVAHDVIANSAIVPLSATGELCVFTTSAADFALDIAGYVGADSPLATIAPARYL
                     DTRTAGGSNTVDGLAQGDGRVAAGTVRQLQIGGRGDVPADAQAAIVNITAVSPSAAGF
                     LTVFDCGTRPLASTVNYLTGQVVPNGAVATLSATGALCIYSLAATDLVVDVSGYVPAD
                     AVGIETMTPSRLLDTRQGEPTVDAAFAGEGRVGAGQFIEVDIAGRADVPADATAAIVN
                     VTVVNPAGPGYVTVYPCGTLPLASNINYQSAGQVQAGNSIAKLSATGTLCLYTLAETD
                     LVLDVTGWIK"
     gene            1518..>2661
                     /locus_tag="PLV68_05565"
     CDS             1518..>2661
                     /locus_tag="PLV68_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00745.1"
                     /translation="MANQTQLTVTFSAPTGLTDAQRTNPNVFIQASIGGTILIEESVQ
                     FNAANGFSVTRVTSVAGTTSGTIFVGAFALASWGDGTPSEGSPTIVQVGLSGVCGVLQ
                     SDLRPPSISASVSCIGGAVAVEWTAKAWNGPNDASKTNSAVKVVVSGAVNAPVTDGAF
                     NAANDFTLEGVAFAPGNTSGAVAVTVTVIADWGDGAPPIPPSAITTLQLAGDCTAPTG
                     PHTFSLPVTLTCTAGNYDVHYTAIASDYDGSTAEARSNPFVFVTLSQPFDVTSQLGAF
                     EPTNNFSFDGHFTVPGNTTGPVILVSGVLSDWGDGAPVPSPPQPSVVTIVNASGDCTP
                     PTAPGAPTSVAASAGPNEAVVTWAAPVVPLGHQAPTAYTVTASTAGA"
     assembly_gap    2662..2671
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2672..4228
                     /locus_tag="PLV68_05570"
     CDS             <2672..4228
                     /locus_tag="PLV68_05570"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HRE00746.1"
                     /translation="PPDTAMPAPLQCVVSGLPAGQAVDFDVTATNDIGTGAASTSNAV
                     TPTEALSLDLSSDLAAVEVGATVTFTVTAHNAADMATVPFGLEAASLQLQLPPGLDVV
                     AGSSACVGGGDATVAPAGGGGTALDFDARDIEPGADLMCSVAAVATSVGALAVSAHAA
                     ATSAAGDVTHDVSSGPVTVTVTAAQPPEHPPTDVGGNGGDGQIVLHWSAPIEVAGRAP
                     ITGYVATATPGGATCATATTTCTISGLSNGAPYTVTVYAVSGGGPGPVSEPSAPLTPT
                     APPTVTVTAGSGQSDPSNTPWILLLVTFDQPVDGFDVDDLVLGGTAGTAAAIVRLAPV
                     LLETTAESATALPFGDGVYVVEVGNLGGSGTLTVTVHAGAVTNANGQPNAVSPTFSLV
                     YDVTPPVLTAPTAAVTATAAAGATSAVVSWSATTEPDAMVVCSPQSGSAFPVGTTQVT
                     CRATDAVGNVTTSFFDVVVSAAGAPISPPGGLPDAGTAVLVLVALALAVLAVGIALSA
                     AARRRRTFTD"
     gene            complement(4281..4727)
                     /locus_tag="PLV68_05575"
     CDS             complement(4281..4727)
                     /locus_tag="PLV68_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HRE00747.1"
                     /translation="MPLHGEYEPSTFDWVREQVETYEASGGREANTLRDTGIPVIIVT
                     CRGVSSGKIRKFALMRVEHDGEYALVASIGGAPKNPSWYHNLVTEPHVMIQDGPEPHD
                     YDVRLVDGDERATWWQRAAAVFPTYDEYAAKTERTIPVFVATRTSA"
     gene            4726..>5141
                     /locus_tag="PLV68_05580"
     CDS             4726..>5141
                     /locus_tag="PLV68_05580"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HRE00748.1"
                     /translation="MTSSVCAHEHNDVDGYDSEVTMPAQLDPNELVRSSMPLCVALGI
                     TGSEATAERVVLEMSWRADLCTIGGVVHGGALMALADSSGAVCAFLNLPPGAVATSTI
                     ESKTNFLGAVTEGKVVRAVSKPLHAGSTTIVVETELR"
     assembly_gap    5142..5302
                     /estimated_length=161
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5303..>5550)
                     /locus_tag="PLV68_05585"
     CDS             complement(<5303..>5550)
                     /locus_tag="PLV68_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HRE00749.1"
                     /translation="PSGVPTWSHHGIICTGETYKDKVKFTFAQGASIDDPDRLFNASL
                     DAGTRRAIDIPEGVTLDADAFIALAQRAAELNTTRAAT"
     assembly_gap    5551..5560
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          892 a         2000 c         2054 g         1012 t
ORIGIN      
        1 caacgccgga ctcgtcagcc ccgtgcgtcc ggaacccgag ccggaaccgg aacccgagcc
       61 cgacccggcg gtcgtcaacc cgtcggcacc ggctcggtac tgggacaccc gcaacgagcc
      121 gaccctggac acgcagttct ccaacaccgg ccgggtgcag gcgggcgcga cgtacaccgt
      181 gcaggtgagc ggccgcggcg acgtgccggc gggggccaaa gcggtcgtcg ccaatctggt
      241 ggccatcgat ccgggcaccg ccgggtacgc gaccatgttc ccgtgcacgg tgacgcctcc
      301 cgtggcctca cacctcaact tcgtggccca cgacgtgatc gccaactccg ccatcgttcc
      361 gctctcggca acgggtgagt tgtgcgtgtt cacaacgtcg gccgccgact ttgcgctcga
      421 catcgccggg tacgttggag ccgactcgcc gttggccacc atcgcacccg ctcggtacct
      481 cgacactcgt acggctggcg gatcgaacac ggtcgacggg ctggcccaag gtgacgggcg
      541 agtggcggcg gggacggtgc gccaactcca gatcggaggc cgcggcgacg tgccggctga
      601 cgcgcaggca gcgatcgtca acatcaccgc ggtgagcccg agtgctgccg ggttcctcac
      661 ggtgttcgac tgcgggacgc gtccgttggc cagcaccgtc aactatctga ccggccaggt
      721 cgtgcccaac ggtgcggtgg ccacgctcag cgcgacggga gccttgtgca tctactcgct
      781 tgctgcgaca gacctcgtcg tcgacgtcag cggttacgtc ccggccgatg cggtgggcat
      841 cgaaacgatg acgccgtctc gcctgctcga cacccgtcag ggcgagccga ccgtcgacgc
      901 tgcctttgcc ggcgagggcc gcgtcggggc aggtcagttc atcgaggtcg acatcgctgg
      961 tcgggccgat gtgcccgccg acgcaacggc cgcgatcgtg aacgtcaccg tcgtcaaccc
     1021 ggctggtccc ggctacgtca ccgtgtaccc gtgtggcacg ctgccgctgg cctccaacat
     1081 caactatcag tcggccggcc aggtccaagc gggtaactcg atcgccaagc tctccgcgac
     1141 gggcacgctg tgcctgtaca ccctggcaga gaccgacttg gtgctcgacg tgaccggctg
     1201 gatcaagtag ccgctgcagg tcggagccga agctccccct ctccgtaccc actgacagag
     1261 agccctgctc gcacatctcc gtgcgagcag ggctatcgtc gtgatcacac gacgtcagac
     1321 ggattgtgac gcaccgttgc caggtgcgcg ctgtgggtct acgtttgcgc cgcatggaca
     1381 ggaccgggcc ttcccgtcac gtgcttgtcg tgatgatcgt cgtcgcgtcg gcgatcacag
     1441 tcgcgacgtc gctcgtgcca gcgacgcggg tgcaggcggc cggccgaccg gtggtcgtgc
     1501 aaacatctgt cgcgtgcgtg gcgaaccaga cacagctgac ggtcactttc agcgcaccga
     1561 ccgggctcac cgacgcccag cgcaccaacc ccaacgtgtt catccaagcg tcgatcggcg
     1621 ggacgattct gatcgaggag tccgtccagt tcaacgccgc caacggtttc tcggtgacgc
     1681 gggtgacctc ggttgccggt acgacgagcg gcacgatctt cgtcggtgcg ttcgcgttgg
     1741 ccagttgggg cgacgggacc ccctctgagg gttcgcccac catcgtgcag gtcggactgt
     1801 ccggtgtgtg tggcgtgttg cagagcgacc tgcgcccgcc atccatcagc gcgagcgtct
     1861 cgtgcatcgg tggcgctgtc gcggtcgagt ggaccgccaa ggcgtggaac ggcccgaacg
     1921 acgcgtcgaa gacgaactcc gcggtcaagg tggtggtgag cggcgctgtc aacgcgccgg
     1981 tcactgacgg ggcgttcaac gcggccaacg atttcacgct ggagggggtg gcgttcgccc
     2041 ctggcaacac ttccggggct gtagctgtca ccgtcacggt cattgccgac tggggcgacg
     2101 gagctccgcc gattccgccg tcggcgatca ccacgttgca gttggccggc gattgcaccg
     2161 cgccgactgg cccgcacacc ttcagcctgc ccgtcacgtt gacgtgcacc gcgggtaact
     2221 acgacgtgca ctacaccgcc atcgcctcgg actacgacgg ctctacggcc gaagcgcggt
     2281 cgaacccgtt cgtgttcgtc acgttgagcc aaccgttcga tgtgacgtcc caactcggcg
     2341 ccttcgagcc gacgaacaac ttcagcttcg acggccactt caccgtgccc ggcaacacca
     2401 caggcccggt gatcctcgtc agcggcgtgc tcagcgactg gggcgacggc gcgccggtgc
     2461 cgtcaccgcc gcagccgtcg gtcgtcacga tcgtcaacgc gagtggcgac tgcacgccgc
     2521 ccaccgcgcc gggggcgccg acgtcggtcg cggcgagtgc tggccccaac gaagcggtcg
     2581 tcacctgggc cgcgccggtg gtccccctgg gccatcaggc accgactgcg tacacggtca
     2641 cggcgtcgac ggcaggggcg tnnnnnnnnn nccaccagac acggcaatgc cggcgccgtt
     2701 gcagtgcgtc gtctccggtc tgccggccgg tcaggcagtg gacttcgacg tgacggcgac
     2761 caacgacatc gggacgggtg cggcgtctac ctcgaacgca gtgaccccga ccgaagcttt
     2821 gagcctcgac ctcagcagcg acctcgccgc ggtcgaagtc ggtgcgaccg tgacgttcac
     2881 ggtgacggcg cacaacgctg ccgacatggc caccgtgccg ttcggtctcg aagcggcatc
     2941 gttgcagttg caactaccgc ctggattgga tgtggtggct ggttcgtcgg cttgtgtcgg
     3001 tggcggcgat gcgacggtgg ctcccgccgg aggcggcggc accgcgctgg atttcgacgc
     3061 tcgggacatc gaaccaggag cggatctgat gtgctcggtc gctgccgtcg cgacttctgt
     3121 cggagcactg gctgtttcgg cccacgcggc ggcaacgagc gcggctggcg acgtgaccca
     3181 cgacgtgtcg agcggaccgg tcaccgtcac cgtgaccgct gcccagccac cagagcatcc
     3241 accgaccgat gtcgggggca acggtggcga cggccagatc gtgctgcact ggagcgcccc
     3301 gatcgaggtc gccgggcggg caccgatcac gggatacgtc gcgaccgcaa cgcccggcgg
     3361 ggcgacgtgt gccaccgcga ccaccacctg caccatctcc ggtctcagca acggcgcccc
     3421 atacaccgtc acggtgtacg ccgtcagtgg tgggggaccg gggccggtgt cggagccgtc
     3481 ggctccgctc acaccgaccg cgccaccaac cgtgacggtg actgcgggca gcggccaatc
     3541 cgacccgagc aacacgccgt ggatccttct cctcgtcacc ttcgaccaac ccgtggatgg
     3601 gtttgacgtc gacgacctgg tgctcggcgg cacggctggc accgcggccg cgatcgtgcg
     3661 cctcgcgcca gtcctcctgg agaccaccgc cgagtcagcg acagctctgc ccttcgggga
     3721 tggggtctac gtcgtcgagg tcggcaacct tggtggctcg ggcacgctga ccgtcaccgt
     3781 gcacgccggt gcggtgacga acgccaacgg acagcccaac gcggtgtccc cgacgttcag
     3841 cctggtctac gacgtcacgc caccggtcct gaccgctccc accgcggcag tgacagcgac
     3901 cgcagccgcc ggagcgacgt cggcggtcgt gtcctggtcc gcgacgacgg agccggacgc
     3961 gatggtggtc tgctcacccc agtcgggtag cgcgtttcct gtcggtacga ctcaggtcac
     4021 gtgccgggcc accgatgccg tgggcaacgt gaccacgtcg ttcttcgacg tcgtcgtgtc
     4081 ggccgcgggc gcgccgatca gcccgcccgg cggtctccca gatgccggca ccgcggtgct
     4141 ggtgctcgta gcgctggccc tcgcggtgtt ggctgtcggc atcgcgctct cggccgcggc
     4201 gcgacggcgt cgcacgttca ccgattgact ccgacgaact cctcggtaca ccgccatggt
     4261 ggcgaggtac cgagaactgg ttacgcgctg gtgcgggtgg cgacgaagac gggaatggtc
     4321 cgttcggtct tggctgcgta ctcgtcgtag gtggggaaca cagccgcggc gcgctgccac
     4381 caggtcgctc gttcgtcgcc gtcgacgagg cgcacgtcgt agtcgtgcgg ttcggggccg
     4441 tcttggatca tcacatgggg ttcggtgacc aggttgtgat accagcttgg gttcttcggt
     4501 gcgccaccga tcgacgcgac gagtgcgtac tcgccgtcgt gctcgacgcg catcagcgcg
     4561 aacttgcgga tcttgcccga cgacacacct cggcaggtca cgatgatgac cgggatgccg
     4621 gtgtcgcgca gcgtgttggc ttcgcgtccg ccggaagcct cgtaggtttc cacctgctcg
     4681 cgcacccagt cgaacgtgct cggctcatac tctccatgca acggcatgac gtccagcgta
     4741 tgcgcccacg agcacaatga tgttgacggc tacgactcgg aggtcaccat gccagcccaa
     4801 ctcgacccca acgaactggt ccgatcatcg atgccgctgt gtgtcgcgct cggtatcacc
     4861 gggtcggagg cgactgctga acgggtcgtg ctggagatga gttggcgcgc cgatctgtgc
     4921 acgatcgggg gcgtggtgca cggtggagcg ctgatggcgc tggccgactc gtcgggtgcg
     4981 gtctgcgcgt tcttgaacct gccccctggg gcggttgcga catccacgat cgagtcgaag
     5041 acgaacttct tgggtgcggt caccgagggc aaggtcgtca gggcggtgtc gaagccgttg
     5101 catgccggtt cgaccacgat cgtcgtcgag accgagttgc gnnnnnnnnn nnnnnnnnnn
     5161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5281 nnnnnnnnnn nnnnnnnnnn nnttggtcgc ggcgcgggtg gtgttgagtt cggcggctcg
     5341 ttgggcgagg gcgatgaagg cgtctgcgtc gagcgtgacg ccttcgggga tgtcgatcgc
     5401 gcggcgggtg cccgcgtcga ggctggcgtt gaagaggcgg tcggggtcgt cgattgatgc
     5461 gccctgggcg aaggtgaact tgaccttgtc cttgtaggtc tccccggtgc agatgatgcc
     5521 gtggtgcgac cacgtgggaa cgccgctcgg nnnnnnnnnn cgagggtttg acccatttca
     5581 cctcttcggt cacatccgga atcgcctgtt ggatcagtcc acggatgcgg ttcaaggtgt
     5641 cactgcgcca gtcagaactc gccatgtcgg tcggcctccg cgtgtctcgt cgcgtgaggc
     5701 accgacgctg ccacgaccag accaaacctg tcaacgccgg cgatggcgac ctcgcggcgc
     5761 gcgatcgcgt gttgcccgtc gatgaacgag ttgtctcgac gtggtctgcc cctggacctg
     5821 atccggtgag accgaggccc tgcgtaaaac ggctgtcggg gcggccgccg tgcagcaggt
     5881 acgttcgcgg gacatgcggg gggtgagttg gccgacggtt ggcagggacg agcagcttcg
     5941 cgtcgtcggt gatgcggtcg ggcgggcaat cgaaggggtc ggcagtgtcg tggccgtgga
     6001 ggcaccggcc ggggcgggca agacccaact cctggacgaa gccgcagcgt cggcgaaggg
     6061 gtcgggggtc gcggtgcgcc gggcgcgtgt gcccagcggc ggtgtcgctg cgccgttctc
     6121 cgcgccactc gccttgttc
//
LOCUS       DAORNF010000102         6083 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_9624_length_6083_cov_1.701228, whole genome shotgun sequence.
ACCESSION   DAORNF010000102 DAORNF010000000
VERSION     DAORNF010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 6083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6083
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9624_length_6083_cov_1.701228"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..508)
                     /locus_tag="PLV68_05590"
     CDS             complement(<1..508)
                     /locus_tag="PLV68_05590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00750.1"
                     /translation="MTNTINKFAEQGGRLKTDDIDFDAFRSEPLSAAVLRCLSYMHDI
                     EYQTVLYTRELLMMPQWKDPEFTSFLTLWNYEEYWHGDAIGRVLEAHDWPSHDERLIA
                     MRRFNKRYLQWSPAIFWTLSRVVRRFPAVHMTWGAINEWTANAAYNRLAALSAHPVMA
                     ELLHRIQRQ"
     gene            complement(588..>1245)
                     /locus_tag="PLV68_05595"
     CDS             complement(588..>1245)
                     /locus_tag="PLV68_05595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00751.1"
                     /translation="VINNIERVASLFLVKTTYALFFAIFTIVSGSAFPFLPRHLTLVG
                     TLTIGTPAFFLALAPNDQPVRAGFLGRVLRIAIPGGLLASVSTFIAYTIARQSDLDPD
                     PDRALAMARTTATIVLAACAILILVRVARPFAVWKIALVTIMAVFIALTMIVPFLRDF
                     FEIVMPPSGTVVMMTVVVAITAALLPVVWRVGERAVGWGEQVLQRRREGRVDTSAPAS
                     "
     assembly_gap    1246..1286
                     /estimated_length=41
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1287..>1610)
                     /locus_tag="PLV68_05600"
     CDS             complement(<1287..>1610)
                     /locus_tag="PLV68_05600"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HRE00752.1"
                     /translation="PRTVSAVGRRVGLAGADRHTDARNLPDDLDALADAMDESTIFGR
                     VTPHQKRAMVHALQRRGHVVAMTGDGVNDVLALKDSDCGIAMASGSEATRAVAQVVLL
                     DSSFS"
     assembly_gap    1611..1620
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1621..>1961)
                     /locus_tag="PLV68_05605"
     CDS             complement(<1621..>1961)
                     /locus_tag="PLV68_05605"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00753.1"
                     /translation="PGWQVTASVAFSSARKWSGSSFGSNGTWVFGAPEMVLADHPDDA
                     LVNRVEAGADAGKRVLLLAHSNRALDGEALPADLTPAALVLLEDQVRPDAEATLDYFA
                     KQGVTLKVISG"
     assembly_gap    1962..2045
                     /estimated_length=84
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2046..3087)
                     /locus_tag="PLV68_05610"
     CDS             complement(<2046..3087)
                     /locus_tag="PLV68_05610"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HRE00754.1"
                     /translation="MPAPSDRVTSGRVTPDQSTLDPAVTGRAERGLTTAEVADRVARG
                     LTNDVPDAPSRTITEIIRANLFTRFNALIGSLFAIVMICGAYRDGLFGGVILANTLIG
                     IVQELRAKRTLDRLTVINAPKVTAIRDGEAVHLSVGELVMDDIVDLHPGQQIVADSTV
                     VTCTNLEVDESLLTGESDPIVKQPGDELLAGSFVVAGNARATVAKVGADAYAAQLAEE
                     ARRFTLVHSELRASIDRIVTLITYVLVPTGIGLFISQLSRDHNSVRDGLVAAVGGVVA
                     MVPEGLILLTSVAFTVGVVRLARRRTLVQELPAIEVLARVDVICLDKTGTITSGAMDL
                     ASVEPLGTSLSEA"
     gene            3275..>4576
                     /locus_tag="PLV68_05615"
     CDS             3275..>4576
                     /locus_tag="PLV68_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRE00755.1"
                     /translation="MPRPSGSFSRGGFTRDTSVVQHRLARGTVRRVLTFAKPYRLWLC
                     AFVALIVVDALIGAAGPLLFRAIIDDGIGQGRRDVVIGLAIVVGALAVVAALNGLAQR
                     WFSARIGEGLIYDLRTQVFDHVQRMPIGFFTRAQTGALVSRLNGDVQGAQQAFTSTLS
                     NVVGNVVGVTATLTAMFVLSWQVTLLALVMLPLFVIPARIVGRKLARITRERYQLNGD
                     MGQMMTERFNVSGALLVKLFGDPAAESAGFGARAARVRDIGVTQAMYTRVLMSVLTLL
                     AALATAIAYGLGGIMAIGGALGVGTVVALTAYLAQLYGPLTSLSNLQVDVMTTLVSFE
                     RVLEVLDLQPTIADAPDARPLPTGPVGVEFDHVGFRYPTAAEVSLASLEGVARLDGHQ
                     STPVLRDVSLAVGAGQMLAIVGPSGAGKTTLAGLVARLYDPT"
     assembly_gap    4577..4633
                     /estimated_length=57
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4634..5145
                     /locus_tag="PLV68_05620"
     CDS             <4634..5145
                     /locus_tag="PLV68_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRE00756.1"
                     /translation="VVSQDAHLFHDTLRANLVYAKPDATDEQLIAALRAAQIWHVIER
                     LPDGLDTVVGDRGHRLSGGEKQRIAIARLLLKDPAVVVLDEATAHLDSESERAVQAAL
                     DAALVERTSIVIAHRLATVRAADQIVVVDDGRVVERGDHASLLAADGLYALLCRTQFA
                     AEAEAVVTA"
     assembly_gap    5321..5364
                     /estimated_length=44
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5365..>5705
                     /locus_tag="PLV68_05625"
     CDS             <5365..>5705
                     /locus_tag="PLV68_05625"
                     /inference="COORDINATES: protein motif:HMM:NF013565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HRE00757.1"
                     /translation="ATLVYDILRRYLEFTGLHVRHVSNITDIEDKIIDRATREGRPWQ
                     DITTACEAIWWQAMDAMGVLRPTDIPHATAYVDGMVTMIGELLDRGAAYTTDDGIYMD
                     VSTVDDYGLLA"
     assembly_gap    5706..5765
                     /estimated_length=60
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5766..>6083
                     /locus_tag="PLV68_05630"
     CDS             <5766..>6083
                     /locus_tag="PLV68_05630"
                     /inference="COORDINATES: protein motif:HMM:NF013565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HRE00758.1"
                     /translation="RAAADFALWKFTPSHGDRRGEPSWPSPWGAGRPGWHSECVVMSL
                     ELLGEHFDLHTGGQDLKFPHHENERAQAAALGKGFANHWMHHAFVVDGEGEKLSKSLG
                     NFTN"
BASE COUNT          909 a         1926 c         1992 g          960 t
ORIGIN      
        1 cttggcgttg aatgcggtgc agcaactcgg ccatcaccgg atgcgccgag agcgcggcca
       61 agcggttgta ggccgcgttg gcggtccact cgttgatcgc accccacgtc atgtgcacgg
      121 cggggaagcg ccgcacgacg cggctgaggg tccagaagat cgccgggctc cactgcaggt
      181 agcgcttgtt gaagcgccgc atcgcgatca gacgctcgtc atgggacggc cagtcgtgag
      241 cctcgagcac tcggccgatc gcgtcgccgt gccagtactc ctcgtagttc cacagcgtca
      301 ggaaggacgt gaactcgggg tccttccact gcggcatcat caacagctcg cgggtgtaga
      361 gcaccgtttg atactcgatg tcgtgcatgt acgacagaca gcgcagcacg gcagccgaca
      421 gcggttcgga tcggaacgcg tcgaaatcga tgtcgtcggt cttcagccgt ccgccctgct
      481 cggcgaactt gttgatcgtg ttggtcatct caccccgtct ccttccgggt gctcctgcac
      541 agacatgatg ccagtattcg tgatggcggt gttcgtgatg ccggtgctca cgatgccggt
      601 gcggacgtgt cgacccttcc ctcgcgtcgg cgttgtaaga cctgttcgcc ccaaccgacg
      661 gcgcgctcgc cgacccgcca gaccacgggc agcaacgcgg cggtgatcgc gacgaccacc
      721 gtcatcatca cgacggtgcc ggacggcggc atgacgatct cgaagaaatc gcgcaggaac
      781 ggcacgatca tcgtcagggc gatgaacaca gccatgatcg tgacgagggc gatcttccac
      841 acggcgaacg gacgggcgac gcgcacgagg atgaggatcg cgcacgccgc gagcacgatc
      901 gtcgccgtgg tgcgggccat cgccagcgct cgatcgggat cggggtcgag gtcggactgg
      961 cgggcgatgg tgtaggcgat gaacgtcgac accgacgcga gcagcccccc ggggatcgcg
     1021 atgcgcagca cccgccccag gaaccccgcc cgcaccggct gatcgttggg cgcgagcgcc
     1081 aggaagaacg cgggcgtgcc gatggtcagc gtgccaacca gggtcagatg gcggggcagg
     1141 aatgggaacg ccgaaccgct gacgatggtg aagatcgcga agaacagcgc ataggtggtc
     1201 ttgacgagga acaggctggc gacgcgctcg atgttgttga tcaccnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnagct gaacgagctg tcgagcagca caacctgggc
     1321 cacggcgcgg gtggcttcgg agccgctggc catcgcgatg ccgcagtctg aatccttcaa
     1381 cgcgagcacg tcgttgacgc cgtcgccggt catcgccacg acgtggccgc gccgttgcag
     1441 ggcgtgcacc atggcccgtt tctggtgcgg cgtgacgcgg ccgaagatgg tcgactcgtc
     1501 catcgcgtcg gcgagcgcgt cgaggtcgtc gggcaggttg cgggcgtcgg tgtggcggtc
     1561 ggccccggcc agcccgacgc ggcgaccgac ggcgctgacc gtgcgcgggt nnnnnnnnnn
     1621 gccggagatc accttcaacg tgacgccttg cttggcgaag tagtccagcg tggcctcggc
     1681 gtcggggcgg acctgatctt ccaacagcac cagggcggcg ggcgtcagat cggcgggtag
     1741 cgcctcgccg tcgagcgccc ggttgctgtg cgccagcaac agcacccgtt tgcccgcgtc
     1801 ggcgcctgct tccacccggt tgaccagcgc gtcgtcggga tgatcggcca gcaccatctc
     1861 gggcgcaccg aagacccagg taccgttgct gccgaagctc gacccgctcc acttgcgggc
     1921 cgacgaaaac gcgaccgacg cggtcacctg ccaccccggc gnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnncggcc tcggacaacg acgtgccgag cggttcgacc gaggccaggt ccatcgcccc
     2101 ggacgtgatc gtgccggtct tgtcgagaca gatcacgtcg acgcgggcga gcacttcgat
     2161 cgccgggagc tcttgcacca gcgtgcggcg ccgggccagg cgcactacgc cgaccgtgaa
     2221 ggcgacgctg gtcagcagga tcagcccctc gggcaccatc gccacgacac cgccgaccgc
     2281 ggcgaccagc ccgtcgcgca ccgagttgtg atcccgcgag agctggctga tgaacagccc
     2341 gataccggtc ggcacgagca catacgtgat cagggtgacg atgcggtcga tcgacgcgcg
     2401 cagttcgctg tgcaccagcg tgaagcggcg cgcttcctcg gcgagttggg cggcgtacgc
     2461 gtcggcaccg accttggcga ccgtggcgcg cgcgttgccc gccaccacga agctgcctgc
     2521 gagcagttcg tcgcccggct gcttgacgat ggggtcggac tcgccggtga gcagcgactc
     2581 gtcgacttcg aggttggtgc aggtgacgac ggtgctgtcg gccacgatct gctggccggg
     2641 atgcaggtcg acgatgtcgt ccatcaccag ttcacccacg ctgaggtgca ccgcctcgcc
     2701 gtcgcggatg gccgtgacct tgggggcgtt gatcacggtg aggcgatcga gggtgcgctt
     2761 ggcgcgcagc tcttggacga tgccgatcag cgtgttggcc aggatcactc cgccgaacag
     2821 gccgtcgcgg taggcgccgc agatcatcac gatcgcgaac agcgacccga tcaacgcgtt
     2881 gaagcgggtg aacaggttgg cgcggatgat ctcggtgatg gtgcgcgacg gagcgtcggg
     2941 gacgtcgttg gtgagcccgc gggccacgcg gtcggcgacc tcggcagtgg tcaggccgcg
     3001 ctcggcgcgg ccggtcaccg cagggtcgag cgtggactgg tcaggggtga cgcggccgga
     3061 cgtgacgcgg tcggacggtg ctggcacccg gcgcagcgta ctcggcccgc tacaacccga
     3121 accggtgtcc gggaatagca ggcgccgcga actcgttgca tcacccgcca cgtgaaaccc
     3181 cacacgaata cccctcacct ctgaatgatc cacacattgt gtgcaatgcc gtaccccgtc
     3241 ggtgaccgat cggtacgaag aaaggcggcc ccatatgccc cgaccctctg gttccttttc
     3301 tcgcggtggc ttcacccgcg acacgtccgt cgtccagcac cgcctcgctc gcggcaccgt
     3361 ccgccgcgtc ctcaccttcg ccaagccgta tcggctctgg ctctgcgcct ttgtcgcctt
     3421 gatcgtggtc gacgcgctga tcggcgctgc cggtccgctg ctgttccggg cgatcatcga
     3481 cgacggcatt ggccagggcc gtcgtgacgt cgtgatcggt ctggccatcg tcgtcggtgc
     3541 tctcgccgtg gtcgccgcgc tcaacggctt ggcccaacgc tggttctcgg cccgtatcgg
     3601 cgagggcttg atctacgact tgcgaacgca ggttttcgac cacgtgcagc ggatgccgat
     3661 cgggttcttc acccgtgctc agaccggtgc gctggtcagc cggctcaacg gcgacgtgca
     3721 aggcgcgcaa caggcgttca cgtccacgtt gtcgaacgtg gtcggcaacg tcgtcggcgt
     3781 gaccgcgacg ctgacggcga tgttcgtgct gtcgtggcag gtgaccctgc tcgcgctggt
     3841 gatgctgccc ctgttcgtga tccccgcccg catcgtcggt cgcaagttgg cgcgcatcac
     3901 ccgcgagcgc taccaactca acggtgacat gggccagatg atgaccgagc ggttcaacgt
     3961 gtccggcgct ttgttggtca agctgttcgg cgatcccgcg gccgagtcgg ccggtttcgg
     4021 tgcccgggcg gcgcgggtgc gcgacatcgg ggtcacccag gccatgtaca cgcgggtgct
     4081 gatgtcggtg ctgacgctgt tggccgcgtt ggctaccgcg atcgcttacg gactgggcgg
     4141 catcatggcg atcggcgggg cgctcggcgt cggcacggtg gtcgcgctga ccgcctacct
     4201 ggcgcagctc tacggtccgc tgacgtcgtt gtcgaacctg caggtcgacg tgatgaccac
     4261 gctggtcagc ttcgagcgag tgctcgaggt gctcgacctc cagccgacca tcgccgatgc
     4321 ccccgacgct cggccgctgc cgaccgggcc ggtcggggtc gagttcgatc acgtcggctt
     4381 ccgctacccg accgcggccg aggtgtcgct ggcctcgctg gaaggggtcg cccgcctcga
     4441 cggccaccag tcgacccccg tgctgcgtga cgtcagcctt gccgtcggcg ccggacagat
     4501 gctggcgatc gtcggcccgt cgggcgcggg caagaccacg ctcgccgggc tggtcgcccg
     4561 gctgtacgac ccgaccnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4621 nnnnnnnnnn nnngtgtggt gtcgcaggac gcccatctgt tccacgacac gctgcgcgcc
     4681 aacctggtct acgccaagcc cgatgcgacc gacgaacagc tgatcgcagc gctgcgcgcg
     4741 gctcagatct ggcacgtgat cgaacgcctg cccgatgggc tcgacacggt ggtcggcgat
     4801 cgcggccatc gcctgtccgg cggtgagaag cagcggatcg cgatcgcccg cctgttgctg
     4861 aaggacccgg ccgtcgtcgt gctcgacgaa gcgacggcgc atctcgactc tgagtccgag
     4921 cgggcggtgc aagccgcgct cgacgcggca ctggtcgagc ggacctcgat cgtgatcgcc
     4981 catcgcttgg ccaccgtgcg cgccgccgac cagatcgtgg tcgtcgacga cggccgcgtg
     5041 gtcgagcggg gcgatcatgc cagcttgctc gccgccgacg gcctgtacgc gttgttgtgc
     5101 cgcacccagt tcgcggccga ggccgaagcg gttgtcactg cgtaatcacg cggcagcggt
     5161 cgtggcctga catgacctga gacgctgcga actcgttgcc ggtcgaggca tcggggtcaa
     5221 tacgctggcg gccatgttga gcctgtacga caccgcgaca cgtaccgttc gcccgctgca
     5281 aatgcgtgag cccggcaccg tgagcatgta cgtctgcggc nnnnnnnnnn nnnnnnnnnn
     5341 nnnnnnnnnn nnnnnnnnnn nnnngggcca cgctggtgta cgacatcctc cgtcggtacc
     5401 tcgagttcac cggcttgcac gtccgccacg tctccaacat caccgacatc gaagacaaga
     5461 tcatcgaccg ggcgactcgc gaggggcggc cgtggcagga catcaccacc gcgtgcgagg
     5521 cgatttggtg gcaggcgatg gacgcgatgg gcgtgctgcg ccccaccgac atcccccacg
     5581 ccacggccta cgtcgacggc atggtgacca tgatcggtga gctgctcgat cggggtgctg
     5641 cgtacaccac cgacgacggc atctacatgg acgtcagcac cgttgacgac tacggcctgc
     5701 tcgctnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5761 nnnnncgtgc tgcagccgac ttcgcgctgt ggaagttcac gccatcacat ggggatcggc
     5821 gtggcgagcc gtcgtggccg tctccctggg gggccgggcg ccccggctgg cacagcgagt
     5881 gcgtggtgat gagcctggag ttgctgggtg agcacttcga cctgcacacc ggtgggcaag
     5941 acctcaagtt cccgcatcac gagaacgagc gggctcaggc cgcggcgctg ggcaaggggt
     6001 tcgccaacca ctggatgcac cacgcgttcg ttgtcgacgg cgagggcgag aagctgtcga
     6061 agagcctcgg caacttcacc aac
//
LOCUS       DAORNF010000103         6075 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_9641_length_6075_cov_1.951163, whole genome shotgun sequence.
ACCESSION   DAORNF010000103 DAORNF010000000
VERSION     DAORNF010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 6075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6075
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9641_length_6075_cov_1.951163"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2054)
                     /locus_tag="PLV68_05635"
     CDS             complement(<1..2054)
                     /locus_tag="PLV68_05635"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRE00759.1"
                     /translation="MISAAARKSLTDLTHRRARTVLTVVTLMLAVASISFLAIPTLID
                     DAMQQEVVDGRLADARVFMRPVVLTDEDLAGLAAISNVSVAEPRSSVDVRVMVGERRA
                     PARVIGVRDFARQGVDLVRMETGALPRPGEVLSDVQNANTGVYDGGVGDRITVIGPVG
                     DQELVVSGRGRSLPGGEHVQDESAAVFYADAATVADLSGEPGFSELALRFDDPEPEPA
                     RIALDEVRDHLAGVSGFSGFSDLPELRQPGDWPGRTDTERFGQMISILAVLALLSAIV
                     LISSTMSTLVAEQTRDIAVMRAIGARRRQVAGVYLRTTLLLGAAGAVGGTLLGVVLSS
                     VLANTFAMSFWAVPVGFGVDPMVALASIAAGLLVPSLAALPAIRRSTTVDLREALDAS
                     GSALGRETAVDRGLRRATFLPRGMQIGLRNVGRRKRRTFTTALIVALAVGNLLATMAL
                     AAAATEATRSSWASHLQDVQVWTTGAQPFDQEAEQAIRAVPGVAEVEPVLKNTVALDG
                     DEAFLWAMEPEPLFDHRITGGRWFSVEEDRDGAAVAVIERNLSQRTGVVVGDEVQIDT
                     AAGPARFLVIGTVNNQQENGRVLYIPLTTGRTLLGQRDGATSYLIRAQSPDPAIVDRV
                     TTEVEDALAGLGFEVRSEVTYVAERDEVAANRSLTTTIALLGFVIVATSMVGLANTMT
                     TSV"
     gene            complement(2066..>2493)
                     /locus_tag="PLV68_05640"
     CDS             complement(2066..>2493)
                     /locus_tag="PLV68_05640"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRE00760.1"
                     /translation="GENVGIIFQFFQLLPTLSALENAVLPLDFLRRGSKRERFDRARH
                     NLELVGLGDKADHLPSELSGGEQQRVAIARSMAADPRLIVGDEPTGNLDTTTAGEMFE
                     LLVRLNAEGKTILFVTHDRDLAARAARVIEIRDGLVVEA"
     assembly_gap    2494..2503
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2504..2718)
                     /locus_tag="PLV68_05645"
     CDS             complement(<2504..2718)
                     /locus_tag="PLV68_05645"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRE00761.1"
                     /translation="MAVEKEFQMGKVRYRALRGVDLSIAPGEMVAVVGPSGSGKSTIL
                     NMITSIDRPTSGTVTFDGRRLDQLSEE"
     gene            complement(3268..3609)
                     /locus_tag="PLV68_05650"
     CDS             complement(3268..3609)
                     /locus_tag="PLV68_05650"
                     /inference="COORDINATES: protein motif:HMM:NF020873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein"
                     /protein_id="HRE00762.1"
                     /translation="MNQPCLPDGLEHVRTTDTFDNDTVPAGLLRAHRVADGVWGRLVV
                     HTGTVSFVFDDEPEHPISVSAGDAVAIPPARQHHLELHEAATFAVEFHRLPNMPTPEA
                     GTESTALQGPG"
     gene            complement(3606..>4952)
                     /locus_tag="PLV68_05655"
     CDS             complement(3606..>4952)
                     /locus_tag="PLV68_05655"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HRE00763.1"
                     /translation="AVVVAGGTVVVAILGLQFAGIPFVAALGYSASIVVAVAVLVAIT
                     LLPALLSVARHRILPKHQRTVRQSTEVAHSGWVSWARWVAHHPWRSSIGATVVLVTLA
                     IPMLDMRLGQADAGSDPTTTTHRRAYDLLADGFGDGFNGPLLIAVDLGDSPDPAVLER
                     LSATVAADPGVRAVSPPELNASGDTGLITAIPTTKPQDQATSDLVHRLRNDLLPAATA
                     GTGAEALVGGPTAGFIDQSDKIAARLPWFITAVVSLSFLLLMIVFRSILVPLKAAILN
                     LLSIGAAYGVVVAVFQWGWGRSLIGLDEAVPIASFVPMMMFAILFGLSMDYEVFILSR
                     IREEYHRGHSNIDSVVEGLGATAKVITAAALIMISVFLGFVASDDPVVKMMGVGLATA
                     VAVDATIVRMVLVPSTMALVGDPNWWLPRWLDRILPRLDLEAGPDAIASSDEGVPR"
     assembly_gap    4953..4981
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4982..5684)
                     /locus_tag="PLV68_05660"
     CDS             complement(<4982..5684)
                     /locus_tag="PLV68_05660"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HRE00764.1"
                     /translation="MGLNRVAGGDAVDDFRVPGVESQAAVDLLEQRFPERAGATAMVV
                     FHVDDGTVNDPATAAAIDATIAEVTSLEHVLAVTDPLAGPRAISPDGSTAFAAVQFDG
                     STADLGRATLDELFDTATPAEQAGVQVEFGGELPTVLKERSEGPAEMIGMLAALVVLF
                     VTFRAVVATVMPLGVAVAGLATGLSIVGLLSGLIDIPSIAPRLGTMIGLGVGIDYALF
                     VLSRHRDHLAHGMDVD"
     gene            5851..>6075
                     /locus_tag="PLV68_05665"
     CDS             5851..>6075
                     /locus_tag="PLV68_05665"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HRE00765.1"
                     /translation="MAEPTGRRERNKRERRRRLEDVALELFEREGFDKTTIEQIAVEA
                     ELAPRTFFSYFATKDDLAAMAKDDEADLAMF"
BASE COUNT         1004 a         2000 c         2104 g          928 t
ORIGIN      
        1 acgctggtgg tcatcgtgtt ggccaacccg accatgctcg tggcgacgat gacgaaaccg
       61 agcagggcga tcgtggtcgt gagcgagcgg ttggcggcca cttcgtcgcg ctcggcgacg
      121 taggtgacct cgctgcggac ctcgaagccg agaccggcga gggcgtcctc cacctcggtg
      181 gtgacgcggt ccacgatggc agggtcgggc gactgggccc gtatcagata cgacgtcgct
      241 ccgtctcgct ggccgagcag cgtccggccg gtggtgagcg ggatgtagag gacccgtccg
      301 ttctcctgct ggttgttcac cgtaccgatc accaggaacc gggcgggccc ggcagcagtg
      361 tcgatctgca cctcgtcgcc gacgacgacc cccgtccgct gggacaggtt ccgctcgatc
      421 accgctacgg ccgcgccgtc acgatcctcc tcgacgctga accaccgtcc gcccgtgatg
      481 cggtggtcga acaggggctc gggctccatc gcccacagga acgcttcgtc gccgtccagt
      541 gccacggtgt tcttgagcac gggttcgacc tcggccacac ccggcaccgc ccggatggcc
      601 tgctcggctt cctggtcgaa cggttgcgct cccgtggtcc acacctggac gtcctggaga
      661 tgactggccc acgacgagcg ggtcgcctcg gtggcggcgg cagccagtgc catggtggcg
      721 agcaggttgc cgaccgcgag ggccacgatg agcgcggtgg tgaaggtccg tcgtttccgt
      781 cggcccacgt tgcgcaggcc gatctgcatc ccccgcggca agaaggtggc ccgtcgcagg
      841 cctcgatcga cggcggtctc ccggccgagc gcggacccgc tggcatcgag cgcctcccgg
      901 aggtccaccg tggtgctccg ccggatggcc ggcaacgcgg cgagagacgg gacgagcagg
      961 cccgcggcga tgctggccaa ggccaccatc gggtcgacgc caaagcccac cggtacggcc
     1021 cagaacgaca tcgcgaacgt gttggccagg acgctcgaca gcacgacgcc cagcagggtg
     1081 ccacccaccg cgccggccgc cccgagcagg agggtggtgc gcaggtacac gcccgccacc
     1141 tgacgtcgcc gcgcgccgat ggcgcgcatc acggcgatgt cgcgggtctg ttcggccacg
     1201 agggtgctca tggtgctgga gatcaggacg atcgccgaga gcagggccag cacggcgagg
     1261 atgctgatca tctgcccgaa gcgctcggtg tcggtccggc ccggccagtc gcctggttgc
     1321 cgcagctcgg ggagatcgga gaacccggag aagccggaca ctcccgcgag atggtcacgg
     1381 acctcgtcaa gggcgatccg ggccggttcc ggttccggat cgtcgaaccg tagggccaac
     1441 tcgctgaacc ccggttcacc actgaggtcg gccaccgtcg cagcatcggc gtagaagacg
     1501 gcggcgctct cgtcttgcac atgctcgcca ccgggcagac tgcgcccccg cccgctgacc
     1561 acgagctcct ggtccccgac agggccgatc accgtgatcc gatcgcccac gccaccgtcg
     1621 tagacgccgg tgttggcgtt ctggacgtcg ctcagcacct cgccagggcg cgggagggcc
     1681 ccggtctcca tccgcaccag gtcgacaccc tgtcgggcga agtcgcgcac cccgatgacc
     1741 cgggcgggcg ctcgccgctc ccccaccatg acccgcacgt cgacgctgga ccggggttct
     1801 gcgaccgaca cgttggagat ggcggccagt ccggcgagat cctcatcggt cagcacgacc
     1861 ggtcgcatga acacccgcgc atcggcgagc cggccgtcga ccacctcctg ttgcatggcg
     1921 tcgtcgatca acgtggggat cgccaggaac gagatgctgg caacagccaa catgagggtg
     1981 accaccgtca gcacggtgcg ggcccgccga tgggtgaggt cggtgagcga cttgcgcgcc
     2041 gccgcagaga tcaccgaccg actcctcagg cttccacgac gagtccgtcg cggatctcga
     2101 tgacgcgcgc cgctctggcg gccaggtcac ggtcgtgggt cacgaacagg atcgtcttgc
     2161 cttcggcgtt gagccgcacg agcaactcga acatctcgcc ggccgtcgtc gtatcgaggt
     2221 tgccggtggg ctcgtcgccc acgatgagac ggggatcggc ggccatcgac cgggcgatgg
     2281 ccacccgctg ctgctcaccg ccggagagct ccgacgggag gtggtcggcc ttgtcgccca
     2341 gcccgaccaa ctccaggttg tggcgggccc ggtcgaagcg ctcccgcttg gatccccgcc
     2401 gaaggaagtc cagggggagc accgcgttct cgagcgccga cagggtgggc agcagctgga
     2461 agaactggaa gatgatgccg acgttctcgc cccnnnnnnn nnntgctcct cggacagctg
     2521 gtcgagccgg cggccgtcga aggtgacggt gcccgacgtc gggcgatcga tgctcgtgat
     2581 catgttcaag atcgtggact tgccgctgcc ggaggggccc accaccgcca ccatctctcc
     2641 cggtgcgatc gacaggtcca cgccgcgcag ggcccggtac cggaccttgc ccatctggaa
     2701 ctccttctcg accgccacga gctcgacgat ggggaccgtg cccgtcccac catctccggt
     2761 ctcggctgca ggcgcggtgc tgctcacgag tcggttccgc tcgacgccac ggcccagcct
     2821 ccgtctcggt ccagctgggc catgccgaca gcagatcgca gttccgaccc gatcgcaagt
     2881 ccgatcgtcc cctcagacgc cagagaggcc ctctccggtg ctcggggcgg acctcagacc
     2941 tggttcatca gcggggcaat gtggcagcgc aggatgtgaa ccagcgacct tcgggttatg
     3001 agtgcaccga cgcccgtggg acgtcgtggg cgctcatcgg tgttctccct gctgaacgag
     3061 cgaatcggga ccgccgagca acgctgtcgt aggcaccagt aggaacccgc aggtgctcgt
     3121 cgtgttgtac gactgttgta cgggacagct cccgagcggc gccaccacgc tcgtctgcgt
     3181 tggcgggtgc gagtcgtgct gacacgctgc tgacagcggc ctcgtcctgc ccgccgcgtt
     3241 gtgatcgacg cggatcggac gtcgaggtca tccaggaccc tgaagcgctg tgctctcggt
     3301 accggcctcc ggtgtgggca tgttcgggag gcggtggaac tcgactgcga aggtcgcggc
     3361 ttcgtggagt tcgaggtggt gctgtcgtgc cggtgggatc gcgacggcgt cccccgcaga
     3421 gaccgagatc ggatgctcgg gctcgtcgtc gaagacgaag ctgaccgtgc cggtgtggac
     3481 cacgaggcgg ccccagacgc cgtcggccac gcggtgggct cgcagcagcc cggcgggaac
     3541 cgtgtcgttg tcgaatgtgt cggtggtgcg gacgtgttcg agcccgtcgg gcagacatgg
     3601 ttggttcatc ggggaactcc ttcatcggac gacgcgatcg cgtcgggtcc ggcttcgagg
     3661 tcgagtcggg gcaggatgcg gtcgagccat cgcggcagcc accagttggg atcgccgacg
     3721 agcgccatgg tggagggcac gaggaccatg cggacgatgg tggcgtcgac ggcgactgcg
     3781 gtggcgagac cgacgcccat catcttgacc accgggtcgt cgctggcgac gaaaccgagg
     3841 aagacgctga tcatgatgag cgcggctgca gtgatgacct tggcggtcgc gccgagccct
     3901 tcgacgacgc tgtcgatgtt cgagtgtcct cggtggtact cctcgcggat gcgggagagg
     3961 atgaacacct cgtagtccat cgacaggccg aacaggatcg cgaacatcat catcggaacg
     4021 aacgaggcga tcgggacggc ctcgtcgagc ccgatgagcg atcgccccca tccccattgg
     4081 aacaccgcga cgaccacgcc gtaggcggcg ccgatcgaca acaggttcag gatcgcggcc
     4141 ttgagcggga cgaggatcga tcggaacacg atcatcagca acaggaacga cagcgacacc
     4201 acggctgtga tgaaccaggg aaggcgtgcg gcgatcttgt cggattgatc gatgaatcct
     4261 gcggtgggtc cgccgacgag tgcctcggct ccggtgcccg ctgtggcggc cgggagcagg
     4321 tcgttgcgga gtcggtggac gagatcgctg gtcgcttggt cttggggctt ggtggtcggg
     4381 atcgccgtga tgagcccagt gtcgccggag gcgttgagct cgggcggtga gacagctcgg
     4441 actcccgggt ctgcggcaac ggtggcgctg agtcgctcga gcacggcggg atcggggctg
     4501 tcgccgaggt cgacggcgat gaggagcggc ccgttgaatc cgtcaccgaa accgtcggcg
     4561 aggaggtcgt aggcacggcg atgcgtcgtg gtggtcggat cagagcccgc gtcggcctgg
     4621 ccgagccgca tgtcgagcat cgggatcgcc aaggtcacga gcacgacggt ggcgccgatg
     4681 ctggagcgcc acgggtgatg cgcaacccat ctcgcccagc tcacccagcc actgtgagcc
     4741 acttcggtcg actgacggac ggtgcgctga tgcttgggga ggatgcgatg acgggcgacg
     4801 ctgaggagag ccggcaggag ggtgatcgcg acgagcacgg cgacggcgac cacgatcgat
     4861 gcggagtacc cgagcgcggc gacgaacggg atcccggcga actgcaggcc gaggatcgcg
     4921 accacgaccg tgccaccggc cacgaccacg gcnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4981 ncgtcgacgt ccatcccgtg tgcgaggtga tcgcggtggc ggctcagcac gaacagcgca
     5041 tagtcgatgc ccactccgag accgatcatc gtgccgaggc gcggtgcgat ggaggggatg
     5101 tcgatcaacc cgctcagcag tccgacgatg gacaaccctg tcgcgaggcc ggcaactgcg
     5161 acgcccaacg gcatcacggt ggcgacgacg gctcggaagg tgacgaagag cacgaccagt
     5221 gcggcaagca tgccgatcat ctcggcggga ccctcggaac gctccttcag cacggtcggg
     5281 agctcgccac cgaactcgac ctgcaccccg gcctgctccg ctggcgttgc ggtgtcgaac
     5341 agctcgtcca acgtggcgcg gccgagatcg gcggtcgagc cgtcgaactg cacggcagcg
     5401 aacgctgtcg agccgtcggg tgagatcgcc cgtgggccgg cgagcgggtc ggtcacggcc
     5461 agcacatgtt cgagagacgt gacctcggcg attgtggcgt cgatcgctgc ggcagtggct
     5521 gggtcgttga cggtgccatc gtccacgtgg aacacgacca tcgcggttgc tccggcgcgt
     5581 tcagggaagc gttgctcgag caggtcgacg gctgcttgcg actccacacc cggcactcgg
     5641 aagtcgtcga ccgcatcacc gccggcgact cggttcaacc ccaccaagcc cagcactgcg
     5701 accacccagg ccacgaggac cagccgatgg tgggcggcga tccaacgacc caatcggtac
     5761 aacccgttcg acacgacaca cctcctgcgc ccaccgtaca tgcagtcact gcatttttgc
     5821 aatggctgct cttttcggta tcctgttcac gtggccgagc cgaccggacg acgagaacgg
     5881 aacaagcgcg agcgccggcg cagactcgag gacgtcgcgc tcgaactctt cgaacgcgag
     5941 ggcttcgaca agacgaccat cgagcagatc gcggtcgaag cagagttggc gccccggacg
     6001 ttcttctcgt acttcgcgac caaggacgac ctcgcggcca tggcgaagga cgacgaggcc
     6061 gacctcgcca tgttc
//
LOCUS       DAORNF010000104         6064 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_9670_length_6064_cov_1.830920, whole genome shotgun sequence.
ACCESSION   DAORNF010000104 DAORNF010000000
VERSION     DAORNF010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 6064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6064
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9670_length_6064_cov_1.830920"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>214
                     /locus_tag="PLV68_05670"
     CDS             <1..>214
                     /locus_tag="PLV68_05670"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRE00766.1"
                     /translation="LTQPVGTGPYVLKSQQRGSSMTFEALPEHWRVVPEFQTITLNLV
                     PEETTRLANLRTGAADIVQLSGSSLAG"
     assembly_gap    215..224
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <225..1038
                     /locus_tag="PLV68_05675"
     CDS             <225..1038
                     /locus_tag="PLV68_05675"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRE00767.1"
                     /translation="LGSDVRTQQTADAWTSHLFPGGMYFDREEYVDVPYADANVRKAM
                     ALAIDREALAQTLYGGAAVPATGWGIYSFSNELTAPPFDPDEAKRLLAEANWPSDYEI
                     TIWVQPSPQLGDTELLSEAIAGMFADVGIKTKLVPIDFAQAQQITSAFQTAGVFTPFA
                     GRLWFDPQPAWQVLFHSGSLYQSFYSEKMDQLLDTLAATNDPEARTAVQKEIHHYMID
                     EQMLTIPLVTGTTVYGLSGDVGEWPALRGATPLYLEYATHADPTGTFRLFEP"
     gene            1107..2075
                     /locus_tag="PLV68_05680"
     CDS             1107..2075
                     /locus_tag="PLV68_05680"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRE00768.1"
                     /translation="MRGLTTKFVTRRSSLTAVDGVNLTLDRGETLGLVGESGSGKSLT
                     CFSIMRLLPHRSAHITAGAVQFEGTDLLSLSDSEIRQLRGRRISMILQDPMTSLNPLF
                     TVGDQLVEGVTTHRSLSRRDARSVARGLLERVQVSAPRSDPDARLRQHPHQLSGGQRQ
                     RVVGALALSGTPSLIIADEPTTSLDVTVQAQFLRLLKDVQRDTGAALLFVTHDLAVVS
                     MVCDRVAVMYAGRIVETGLVRKIFDRPEHPYTQGLLRSVTGGRSGRRLYSIPGQPPAL
                     NDVSTGCSFAPRCEFVVDRCRTEAPPETHLGAGHTVRCWVRRGEHA"
     gene            2068..3051
                     /locus_tag="PLV68_05685"
     CDS             2068..3051
                     /locus_tag="PLV68_05685"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF008453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HRE00769.1"
                     /translation="MPESILELRGLTKHYPVRSGWRGGTLQAVDSIDLVVERGRSVGI
                     VGESGCGKTTTARMMLLLERPTSGEILFEGNDIGAADARAKRRYRREVQPVFQNPYAS
                     LNPRMRVGAIVGEPLQALGHMRGREVDDRVHAALAQVGLPTDAAHRYPHQFSGGQRQR
                     VALARALALRPRLVVLDEPVSALDVSIRAQMLNLLKDLEEELGLTYVLIAHDLPTLRF
                     LVDEVVVMYLGRIVERGPTEQIFSDPRHPYTQALLSAVPALHGGDAARTEVPLAGEVP
                     SPIDPPSGCHFRTRCPHVRDLCEQVVPTLGIVGKSVVACHMVHQPALEVQE"
     gene            3054..>3612
                     /locus_tag="PLV68_05690"
     CDS             3054..>3612
                     /locus_tag="PLV68_05690"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRE00770.1"
                     /translation="MTRYLLVRLGLGIVTIAGMSLLVFLIMRTSGDPVDLYLPQTASV
                     EQRQELRSQLGLDRTLPEQYVTFAADAATGDLGRSISYHLPVWDLISEGLWASIRLTL
                     ISVGIAAFTGVGLGVLAAVHRGSMLDRVARGVALIGMSVPSFFLGIVLILVFAVKMRW
                     LPPGRDEGWTSILLPAVTLAAFPMAG"
     assembly_gap    3613..3742
                     /estimated_length=130
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3743..3968
                     /locus_tag="PLV68_05695"
     CDS             <3743..3968
                     /locus_tag="PLV68_05695"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRE00771.1"
                     /translation="SFLGLVLGPTVGGAVVVEGVFAWPGIGRLAVNAITQRDYPVIQG
                     IVLVITTIVVGVNMTVDVLYRVIDPRLSRG"
     gene            3961..4806
                     /locus_tag="PLV68_05700"
     CDS             3961..4806
                     /locus_tag="PLV68_05700"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRE00772.1"
                     /translation="MADLVTGRRRVPYISLGVLVLVCLVAVFAPVLSPHSPIQNHLSD
                     SLRPPFWQDGGTTRYLLGTDKLGRDILSRALHAARPALIVSLVSITVAAVVGSTLGIV
                     AAYFGGWVDHLVTRAIDIVMSFPAMMLALLLALRFAPGLRSVTTVVVLILWASYARQV
                     RGDAMTVMQKEYIALARSGGRRAIGIMTFHVLPNVVNTIIVVATLQLGIVIILEGSLS
                     YLGAGVPPPDPTWGDMIDDGRGLIESAWWVAIIPGIAIAGLVLAANVVGEWLEERLDP
                     RRREH"
     gene            4837..5805
                     /locus_tag="PLV68_05705"
     CDS             4837..5805
                     /locus_tag="PLV68_05705"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HRE00773.1"
                     /translation="MDLTLHYDFRAPSFGPPVSEIYAAALEQCAWADAVGFTRVVISS
                     HHGCDDDYGPAPLIAASAIAAVTKHMRITPVVALPLHHPLHIAEEVAVLDLVSKGRID
                     LMVVAGYVAAEFAMFGRRMDERGAAMEEGLASIRAAWTGEPFGFRGEQVLVRPRPYQR
                     PGPGLIVGGDSRGAARLGDYYAAMAGGDSWPHYTEACRELGLPEPVQPRGQRAMFVHV
                     TEDPDAAWSTLAPHLLHVNNSYAAWMTQDGRAASYEHADSVDALRSGSVFEVLTPDEC
                     VGYAREWNGLMVDPLFGGIAPDVAWASLELIRDKVLPRLAASEGSN"
     gene            5807..>6064
                     /locus_tag="PLV68_05710"
     CDS             5807..>6064
                     /locus_tag="PLV68_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00774.1"
                     /translation="MTDTYIPNVDEAVLRMYGGLPADRDTSENAGFVVLARTPEADLL
                     LWTSVYAISGRGAGQWIVPDGRIHGTLLLVVPAGDSGPMIDN"
BASE COUNT          973 a         2004 c         1896 g         1051 t
ORIGIN      
        1 cactgacaca gccggtcggt accgggccgt atgtcctcaa gagccagcag cgcggctcgt
       61 cgatgacatt cgaggcgctc cccgagcact ggagagtggt tccagagttc cagacgatca
      121 cgctcaacct cgtccccgaa gagacgacgc gcctcgccaa ccttcggacc ggcgcggccg
      181 acatcgtgca gctctcaggc tcgtcactgg cgggnnnnnn nnnnactggg tagcgacgta
      241 cgcacgcagc agacagccga tgcatggacg agtcaccttt tcccgggcgg catgtacttc
      301 gaccgtgagg agtacgtcga cgtgccgtac gcggacgcca acgtacgcaa ggcgatggcg
      361 ttggcgatcg accgcgaggc cctcgcccag actctctacg gtggcgccgc ggtgccggcc
      421 accgggtggg ggatctactc gttctccaac gaactgaccg ctcccccgtt cgatcccgac
      481 gaagcgaagc gccttctcgc agaggcgaac tggccgagcg actacgagat caccatatgg
      541 gtccagccca gccctcaact gggcgacacc gagttgctct ccgaagcgat cgccggcatg
      601 ttcgccgacg tcgggatcaa gaccaagctc gtgccgatcg acttcgccca ggcacagcaa
      661 atcacctcgg cattccagac ggcaggagtc ttcaccccgt ttgccggccg cctgtggttc
      721 gaccctcagc cagcgtggca ggtgctgttc cacagcggat cgctgtacca gagcttctac
      781 agcgaaaaga tggaccagct gctcgacacg ctcgcggcga cgaacgaccc tgaagcgcgg
      841 accgccgtgc agaaggagat ccaccactac atgatcgacg agcagatgct caccatcccg
      901 ctcgtcacag gaacgacggt ctacggcctc agtggtgacg tcggcgagtg gccagcgctg
      961 cgaggagcca ctcccctgta tctcgagtac gccacccacg ccgatccgac cggtaccttc
     1021 cggctcttcg aaccctgacg ccgatgccag gagccgagcg cgaaccgccc gttgactcct
     1081 gcacgtccag cactccgctc ctggacgtgc gggggctgac gaccaagttc gtcacccgtc
     1141 gttcgagcct cacggcggtc gacggggtga acttgacgct cgatcgcgga gagacgctcg
     1201 gactcgtcgg cgagtctggg tccgggaaga gcttgacgtg cttctcgatc atgcggctgc
     1261 tccctcatcg gtcggcacac atcacagctg gcgccgtaca gttcgagggg accgacctgc
     1321 tctcgctctc cgactccgag atccggcaac tgcgaggtcg ccgaatttca atgatcctgc
     1381 aggatccgat gacctccttg aacccgctct tcaccgtggg cgatcagctg gtcgaaggcg
     1441 tcacaactca tcggtcgctg tcgcgacggg acgctcggtc cgtcgcacgt gggctgttgg
     1501 agcgtgtaca ggtcagcgcg ccacggagcg atcccgacgc gcggcttcgt cagcatcccc
     1561 accaactcag cggcgggcaa cggcaacgag tggtcggcgc tctggcgttg tcggggacgc
     1621 cgtcgctgat catcgcggac gagccgacga cgtccctcga tgtcacggtg caggcgcagt
     1681 tcctgcgcct gctcaaggat gtgcaacgtg acaccggcgc cgcgctgtta ttcgtgacac
     1741 acgaccttgc cgtggtgtcg atggtgtgcg atcgcgtcgc ggtgatgtac gcgggccgca
     1801 ttgtcgagac ggggctggtg cggaagattt tcgaccgccc cgagcacccg tacacgcagg
     1861 gtctgctgcg atcggtgacc ggcgggcgca gcggacggcg gctgtactcc atacccggac
     1921 agcctccagc gctcaacgac gtgagcaccg gatgtagctt cgctccccgt tgcgagttcg
     1981 tcgtcgatcg ctgccgcaca gaggctccgc cagagacgca cctgggtgca ggacacaccg
     2041 tgcgatgttg ggtgagacga ggcgagcatg cctgagtcga tcctcgagtt gcgcgggctc
     2101 accaagcact atccggtgcg gagcggatgg cgcggcggca cgctgcaggc cgtagacagc
     2161 atcgacctag tggtggagcg gggccgctcc gtcggcatcg tcggcgagtc cggctgcggc
     2221 aagaccacga ccgcccggat gatgctgctg ctcgagcgac cgacgagtgg cgaaatcctc
     2281 ttcgaaggga acgacatcgg cgccgcggac gcgcgcgcca agcggcgcta ccggcgtgag
     2341 gtgcagccgg tcttccagaa cccctacgcc tcgctcaacc cgcgcatgcg cgtcggcgcc
     2401 atcgttggcg agccgctcca agcactcggt catatgcgcg gtcgcgaggt cgacgatcgc
     2461 gtacatgctg cactcgccca ggttggcctg cccaccgacg cggcacaccg ctacccgcac
     2521 cagttcagcg ggggacagcg ccaacgtgtc gcgcttgccc gagccctggc cctccgtccg
     2581 cgactggtgg tactcgacga gccggtgtca gcgctcgacg tgtcgatccg ggcacagatg
     2641 ctcaacctcc tcaaagacct tgaggaagaa ctcggcctga cgtacgtcct gatcgcccac
     2701 gacctgccga cgttgcggtt tctcgtggat gaggtggtcg tcatgtacct cggccgcatc
     2761 gtcgagcgag gcccgaccga gcagatcttc agcgaccctc gtcacccgta cacacaggcg
     2821 ctactgtcgg cagtccccgc gttgcacggt ggcgacgcag cgaggaccga agttcccctc
     2881 gcgggtgagg ttcccagccc catcgaccca ccgagtgggt gtcactttcg cacgcgttgc
     2941 ccacacgtcc gcgacctctg tgaacaggtg gtaccgacgc tcgggatcgt cggcaagtcg
     3001 gtcgttgcct gccacatggt ccaccagcct gctctcgagg tccaggagtg accatgacgc
     3061 gctacctctt ggtgcgattg ggtctgggca tcgtcacgat cgctggcatg tcgctgctgg
     3121 tgttcctcat catgcgcacg agcggtgatc ctgtcgacct ctatctgccg cagaccgcct
     3181 cggtcgagca gcgccaggag ctccgctcac agctcggtct ggatcgcacg ctgccggagc
     3241 agtacgtcac cttcgccgcc gacgcagcca ccggtgacct ggggcgctcc atcagctacc
     3301 acctgccggt gtgggatctc atctcggagg ggctttgggc gagcatccgt ctgaccctaa
     3361 tctcggtggg catcgccgca ttcacgggag tcgggctcgg cgtgctcgct gcggtacacc
     3421 gcggctcgat gctcgaccgc gtcgcacgtg gcgtcgcact gatcgggatg tccgtgccgt
     3481 cgttctttct cggcatcgtg ctcattctcg tgttcgcggt aaagatgcgc tggcttccac
     3541 cggggcgcga cgaagggtgg acatcgattc tgctgccggc ggtcacgctc gcggcctttc
     3601 cgatggcggg atnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nngtccttcc tcggcctagt cctcggccca accgtcggtg
     3781 gagcggtggt cgtcgagggc gtctttgcgt ggcctggcat cggccggctc gcagtgaatg
     3841 cgatcaccca gcgcgactat ccggtgatcc aaggcatcgt gctcgtcatc accacgatcg
     3901 tcgtcggcgt gaatatgacg gtcgacgtgc tgtaccgcgt catcgaccca aggctctcac
     3961 gtggctgatc tggtcaccgg ccgacggcgc gtaccgtaca tctcgctcgg cgtgctcgtg
     4021 ctcgtctgcc tggtcgccgt cttcgctccg gtcctcagtc cacactcgcc gatccagaac
     4081 cacctcagcg actcgttgcg gccgccgttt tggcaagacg gcggcaccac ccgctacctc
     4141 ctcggcaccg acaagctggg ccgcgacatc ctcagccgtg cactccacgc ggcacgaccg
     4201 gctctcatcg tctcgttggt ctccatcacc gttgccgcgg tcgtcggcag cacccttggc
     4261 atcgtcgccg catacttcgg cgggtgggtg gatcatctcg tcactcgggc catcgacatc
     4321 gtgatgtcgt ttccagcgat gatgctcgcc ctgctgctcg cattgcggtt cgcgccgggt
     4381 ctgcgcagtg tcaccaccgt cgtcgttctc attctgtggg cgagttacgc ccgccaggta
     4441 cgcggtgacg ccatgaccgt gatgcagaag gagtacatcg cactggctcg ttccggaggg
     4501 cggcgggcga tcggcatcat gacgttccac gtgttaccta acgtggtgaa cacgatcatc
     4561 gtcgtcgcca cgctgcagct cggcatcgtc atcattctcg aaggatcgct cagctacctc
     4621 ggagccggcg tccctccgcc tgacccgacg tggggcgaca tgatcgacga cggccgcggc
     4681 ctcatcgaga gcgcctggtg ggtggcgatc atcccgggca ttgcgatcgc tggattggtg
     4741 ctcgctgcca acgtcgtcgg cgaatggctg gaagagcgac tcgacccccg ccgacgagag
     4801 cactgaaacc cgacgagtac caccgaagga agcaccatgg acctcaccct gcactacgac
     4861 ttccgagcac cgtctttcgg cccaccggtg tcagagatct acgccgccgc gctggagcag
     4921 tgcgcgtggg ccgacgccgt ggggttcacc cgtgtcgtga tctcgtcgca tcacgggtgt
     4981 gacgacgact atgggccagc gccgctgatc gccgccagtg ccatcgccgc cgtcacgaag
     5041 cacatgcgga tcacccccgt cgtcgccctg ccgctgcacc atcccctcca catcgccgag
     5101 gaggtcgcgg tcctcgacct tgtgagcaag ggacgcatcg atctgatggt cgtggccggc
     5161 tacgtggctg ctgagttcgc catgttcggc cgacggatgg acgagcgggg agcggcgatg
     5221 gaggaaggtc tcgcctcgat ccgcgccgca tggacgggcg agccattcgg gttccgcggt
     5281 gaacaggtct tggtacggcc gcggccgtat cagcgacctg ggccggggct gatcgtcgga
     5341 ggtgactcac gcggcgcggc ccgcctcggg gactactacg cagcaatggc cggcggtgac
     5401 tcgtggcccc actacaccga agcgtgccgc gaactcggtc tgcctgagcc ggtgcaaccc
     5461 cgcggtcaac gagcgatgtt cgtgcacgtg acggaggatc cggatgctgc gtggtccacg
     5521 ctggcgccgc atctgctgca cgtcaacaac tcgtacgcgg catggatgac acaggatgga
     5581 cgggcggcgt cgtacgaaca cgccgacagc gtcgacgcgt tgcgctccgg ctccgtcttc
     5641 gaggtgttga cgcctgacga gtgcgtcggg tacgcccgcg aatggaacgg cctgatggtc
     5701 gacccactgt tcggcggcat cgcgccagat gtggcttggg cgagcctgga gctcatccgc
     5761 gacaaggtcc tgccgcgact tgcggcgagc gaagggagca attgagatga ccgatacata
     5821 catcccgaac gtcgacgaag cggtgctccg catgtacggc ggtctacccg ccgaccgcga
     5881 cacgtccgag aacgcggggt tcgtcgtcct cgcccgcaca ccagaggccg atctcctgct
     5941 gtggacgtct gtctacgcga tcagtgggcg cggcgccggg cagtggatcg tgccggacgg
     6001 acggatccac ggaaccctgc ttctggtcgt ccccgcgggt gacagcggcc cgatgatcga
     6061 caac
//
LOCUS       DAORNF010000105         6050 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_9699_length_6050_cov_1.736614, whole genome shotgun sequence.
ACCESSION   DAORNF010000105 DAORNF010000000
VERSION     DAORNF010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 6050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6050
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9699_length_6050_cov_1.736614"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="PLV68_05715"
     CDS             complement(<1..266)
                     /locus_tag="PLV68_05715"
                     /inference="COORDINATES: protein motif:HMM:NF020243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRE00775.1"
                     /translation="MATSTDERGVVVVAGGLGGIGSAIVADLAEQGHRVIAADWRAAD
                     VGSWLEEMVSRFGDRVSFEEVDVTDETSTQGLAGRLAADGIDVP"
     gene            complement(247..1140)
                     /locus_tag="PLV68_05720"
     CDS             complement(247..1140)
                     /locus_tag="PLV68_05720"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HRE00776.1"
                     /translation="MILPTTEIGADPDDIRRYVAAAEEAGYDHLLAYDHVVGADIANR
                     PNWPGPYTYESQFHEPLTLFGFLAAISDLELVTGILILPQRQTVLVAKQAAQIDILSR
                     GKLRLGVGIGWNPVEYESLGMDFATKARRIEEQMYLLRRLWTDPLVNVVTDDHRVVDA
                     GINPLPVQRPIPLWLSGAPLGRIIERVGRLADGWMPLGVPTAAMGEAYERIKQVAAAN
                     GRDPDAIGMQGQLPLVGSAAGRTEELYGRWSAIGVTHIAVNTMNAGLGGVDAHITVIR
                     EAASLLGIAHGKGEADGNVDR"
     assembly_gap    1227..1290
                     /estimated_length=64
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1291..2016)
                     /locus_tag="PLV68_05725"
     CDS             complement(<1291..2016)
                     /locus_tag="PLV68_05725"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRE00777.1"
                     /translation="MAAEMPPSWLTPASVMLTGKVGVVTGAGAGIGKGVATALARFGA
                     DVAICDRDEATLEQTRAEIEELGVRVLAHHLDVRNGDGVTRWLADIGTEFGRVDIVVN
                     NAGGTFQALFADTTAKGEETLIRENFTSATDVIRKALPLISDGASIINITSIEAWRGC
                     PGLSIYGAMKAALENVTLSLALELGDRGIRVNTIAPDSIVSPGGQDVATRPPSEFTLP
                     GLGKGSYVPMTPLRFIGDPDDVAA"
     gene            complement(2022..2987)
                     /locus_tag="PLV68_05730"
     CDS             complement(2022..2987)
                     /locus_tag="PLV68_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRE00778.1"
                     /translation="MAPWEPDNVKGTEMGDLVGKVAIVTGAGRGIGRSEAMTLAAQGA
                     TVVVNDLGGEWDGSGSDTRPAQQVADEIVALGGQASANYDDVSDWAGGQKLIAQAIEQ
                     HGRLDILVCNAGILRDRMTVNMTQQEWAAVLKVHLDGHFVPIRFAAAYWREESKRLGG
                     PVDARLVLTSSESGTYGNPGQLNYAAAKAGIASMGLVLSRELARYGITVNTVCPRART
                     RLNESTFGAFKVDEGLDLWHPDNVAALVGFLCSPRAHDVTGQTFAVGAGDIDLLGGWH
                     VVGHVGKSDEMWSPESVGESIGELFGDRPTVLPSFDFDVPVENKG"
     gene            complement(2995..>3795)
                     /locus_tag="PLV68_05735"
     CDS             complement(2995..>3795)
                     /locus_tag="PLV68_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HRE00779.1"
                     /translation="DEPLTAPAMFSLLAPAYAHEHGLDERQIREVLTRIAWKNHQNGV
                     GNPLAQFRSAVSMETINNAPKVAGMLGVFDCSGVSDGAAAAVVVPAERAYDYTDRPVF
                     VKGLALAAGPCSGTLDPNYAFTSFPEVQASVADAYAQAGITSPRDQLAMAEVHDCFTP
                     TELVLMEDLGFAERGLGWKEVLAGTFDRDGTLPVNTDGGLKAFGHPIGASGLRMLFEC
                     WLQLRREAGERQVATVDQGRNLALTHNLGGPPGGCVSFVGIFGSELSD"
     assembly_gap    3796..4019
                     /estimated_length=224
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4220..4990)
                     /locus_tag="PLV68_05740"
     CDS             complement(4220..4990)
                     /locus_tag="PLV68_05740"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRE00780.1"
                     /translation="MTAVDTPTDTRTVLAEPFQTDVIVKGTGPALVYLHSQYGLRWND
                     YLEQLSQSFTVYAPQVAGEEATLERLDDMLDLLTYLNDVLDALGLGSTAVVGHSLGGA
                     LAAELAAINPNRVSSLVLLSPLGIWSDADPTPDIIGETPADRAARLFVDPASDAGQEW
                     VAGLMSTDRVYFKGMRGYTHWYWPLNEAGLRKRAHRVVAPTLVVHGAADGLTTQRYAE
                     TLAAMLPNATVAPVADASHMLSEKASEVAALTIAHLQS"
     assembly_gap    5118..5245
                     /estimated_length=128
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5246..>6050)
                     /locus_tag="PLV68_05745"
     CDS             complement(<5246..>6050)
                     /locus_tag="PLV68_05745"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HRE00781.1"
                     /translation="HLTPRRRGALRSTLQAVFQDPFSSLDPSQTIGDAIAEPLRVAEE
                     IAMLDILSEGRVVSAFPVGISMDLNFTNGIAASKTRPRYSEAIDLILKSWTQEGPFVY
                     NGRFNKLRYVSPEPLPYQRPHPPVWLLGGESVDSWERAINNDWPFVFFTFNGAQFARP
                     SIEMYWEAVDELGRDANPFRLGYLSLICVGESDAAVERDFAEHVVNFYQLVQGTPLHY
                     FDPPGYKTDRAMKKSGASERLMKSIMEYQATPDNKKWSKILNDGWVVAGT"
BASE COUNT          954 a         1922 c         1832 g          926 t
ORIGIN      
        1 tacggcacgt cgatcccgtc cgccgccaag cggccggcga gaccttgggt gctcgtctcg
       61 tcggtgacgt cgacctcttc gaacgacacc cggtcgccga agcggctgac catctcctcc
      121 aaccaagagc ccacatccgc agcccgccaa tcggcggcga tcacccgatg tccctgttcg
      181 gccaggtcgg cgacgatcgc cgatccgatc ccaccgaggc caccggcgac caccacgaca
      241 cctcgctcat ctgtcgacgt tgccatctgc ctcccccttt ccgtgggcga tgccgagcag
      301 cgaggccgcc tcccggatca ccgtgatgtg tgcatcgaca ccaccgaggc cggcgttcat
      361 ggtgttgacc gcgatgtgcg tgactccgat cgcgctccag cgcccgtaca gctcctcggt
      421 acggccagcc gcggaaccca ccagcggaag ctgcccctgc atcccgatcg cgtccgggtc
      481 ccgcccgttg gcagcggcga cctgcttgat tcgctcgtac gcctcaccca tcgcagcggt
      541 cggtacgccg agcggcatcc acccgtcggc aagacgtccg acccgctcga tgatacgtcc
      601 gagcggcgca cccgacagcc acagcgggat cggccgctgc accggcagcg ggttgatgcc
      661 cgcatcgacc acgcgatggt cgtcggtgac cacgttgacg agcggatcgg tccacaatcg
      721 acgcaagagg tacatctgct cctcgatgcg ccgcgccttg gtcgcaaagt ccatcccgag
      781 cgattcgtac tcgaccgggt tccaaccgat gccgacgccc agccgcagct tgccgcgact
      841 gaggatgtcg atctgcgcgg cctgcttggc gacgaggacg gtctggcgct ggggaaggat
      901 caggatgccg gtgaccaact ccagatcgct gatcgctgcc agaaacccga acaacgtcag
      961 tggctcgtga aactgcgact cgtaggtgta cggccctggc cagttcggcc ggttggcgat
     1021 gtcggccccg acgacgtggt cgtaggcgag gagatggtca tagccggcct cctctgccgc
     1081 agcgacgtag cgacggatgt cgtccggatc ggcgccgatc tcggtcgtcg gcaagatcac
     1141 gccccatcgc accgcagacg acatcactcc accggcggca agacgtcacg acggcggaac
     1201 caaccgccgg cagcatggtt tccaccnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cgcagcgacg tcgtcggggt cgccgatgaa
     1321 gcggaggggc gtcatcggca cgtacgagcc tttgccgaga cccggcagag tgaactccga
     1381 cggaggtcga gtcgcgacgt cctgcccgcc gggcgacacg atcgagtcgg gcgcgatcgt
     1441 gttgacacgg atgccacgat cacccagctc gagcgccaac gagagggtga cgttctccag
     1501 ggccgccttc atcgcgccgt agatgctgag gccgggacaa cctcgccagg cctcgatcga
     1561 ggtgatgttg atgatcgagg cgccatcgct gatcagcggg agcgccttgc ggatgacgtc
     1621 ggtcgcactg gtgaagttct cgcgaatcag cgtctcttca cccttggccg tggtgtccgc
     1681 gaacagcgcc tggaacgtgc caccggcgtt gttgaccacg atgtccacgc gaccgaactc
     1741 ggtgccgatg tcggcgagcc aacgggtcac gccgtcgcca ttgcggacgt cgaggtgatg
     1801 agcgagcacg cgcacgccga gctcttcgat ctcggcgcgg gtctgctcga gcgttgcctc
     1861 gtcacggtca cagatggcga catcggcacc gaagcgagcc agggcggtgg ccacaccttt
     1921 gccgataccg gcgcccgcgc ctgtcacgac gccgaccttg cccgtgagca tcacgctcgc
     1981 gggagtcagc caactggggg gcatctcggc agccatggcc gtcacccctt gttctcgacc
     2041 ggcacgtcga agtcgaagct gggcagcaca gtgggacggt caccgaacag ctcgccgatc
     2101 gactccccca ccgattcggg gctccacatc tcgtcggact tgccgacgtg gccgacgacg
     2161 tgccacccac cgagcagatc gatgtcgcct gcgccgaccg cgaaggtctg gccggtcacg
     2221 tcgtgggcac gcggcgagca caagaagccg accagcgcgg cgacgttgtc ggggtgccac
     2281 aggtcgagac cctcgtcgac cttgaacgcg ccgaacgtcg actcgttgag gcgagtgcgc
     2341 gctcgggggc agaccgtgtt cacggtgatc ccgtagcggg ccagttcgcg ggacaggacg
     2401 agacccatcg aggcgatgcc ggccttggcc gcggcgtagt tgagctggcc ggggttgccg
     2461 taggtgcccg actccgacga ggtgagcacg aggcgggcat cgaccggccc gccgagacgc
     2521 ttcgactcct cccgccagta ggcggcggcg aagcgaatcg gcacgaagtg tccgtcgagg
     2581 tggaccttga gcaccgccgc ccactcctgc tgggtcatgt tgacggtcat ccggtcgcgc
     2641 aggatgccgg cattgcagac caggatgtcg agccttccgt gctgttcgat cgcttgcgcg
     2701 atcagcttct ggccgcccgc ccagtcgctg acgtcgtcgt agttcgcgct cgcctggccg
     2761 cccagcgcga cgatctcgtc ggccacttgc tgcgccggac gggtgtcgct gccactgccg
     2821 tcccactcgc caccgaggtc gttgacgaca accgtcgcac cctgcgccgc caacgtcatc
     2881 gcctccgagc gcccgatgcc acgaccggcg ccggtgacga tggcgacctt gccaacgagg
     2941 tctcccattt cagttccctt cacgttgtcc ggctcccacg gagccatcgc ttgatcaatc
     3001 ggacagttcg ctgccgaaga tgccgacgaa cgagacacat ccgcccggtg gaccaccgag
     3061 gttgtgcgtc agcgccaggt tccgaccctg atcgacagtt gcgacctggc gctcaccggc
     3121 ttcacgacgc aactgcagcc agcactcgaa cagcatgcgc aaccctgacg cgccgatcgg
     3181 gtggccgaac gccttcaggc cgccgtcggt gttgaccggg agcgtcccgt cgcgatcgaa
     3241 ggtcccggcg agcacctcct tccaccccaa cccgcgctct gcaaacccga ggtcttccat
     3301 caacaccagt tcggtcgggg tgaagcaatc gtgcacctcg gccatcgcca actgatcgcg
     3361 cggtgacgtg atcccggcct gggcatatgc atcggccacc gacgcctgca cctccggaaa
     3421 ggacgtgaag gcgtagttgg ggtcgagcgt ccccgagcac ggacccgcgg cgagggccaa
     3481 ccccttcacg aacacgggac gatcggtgta gtcgtatgcc cgctcggctg ggacgacgac
     3541 cgcagccgcg gcaccgtcgg agacgcccga acagtcgaac acgccgagca tcccggcgac
     3601 cttgggggca ttgttgatcg tctccatcga gaccgctgag cgaaactgag ccagggggtt
     3661 gccgacgccg ttctgatggt tcttccacgc gatgcgcgtc agcacctcgc ggatctgccg
     3721 ctcgtcgaga ccgtgctcgt gcgcgtaggc cggggcaagc aggctgaaca tcgccggtgc
     3781 ggtcaacggt tcgtcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt
     4021 cagcgtgccc agccagaacg catcgacgtc gcgcatgccg agacccgccg atgcaaccgc
     4081 gtcggtggtg gcatcgatga gtagatcgtc ggcactcttg gcccagtgct cacggaacgg
     4141 ggtacaaccc atgccgacga tggcgactcg gtcgcggatg cccttcgacg tcatgggtgg
     4201 tgcctcgtgc gtctcggggt caggactgga gatgggcgat cgtcagcgca gcgacctcgg
     4261 acgccttctc cgacagcatg tggctggcat cggcgaccgg tgccaccgtc gcattcggca
     4321 gcatcgccgc caaggtctcc gcataccgct gggtcgtcag cccgtctgca gcaccgtgca
     4381 cgacgagggt cggcgcgacg acgcggtggg cacgcttgcg cagacccgct tcgttcaacg
     4441 gccaatacca gtgggtgtag ccgcgcatcc ccttgaagta cacgcgatcg gtgctcatca
     4501 accctgccac ccactcctgg ccggcatcgc tggccggatc gacgaacagg cgagcggccc
     4561 ggtcggccgg ggtctcaccg atgatgtccg gcgtcgggtc ggcgtcgctc cagatgccca
     4621 gcggcgacag cagcaccagg ctgctcacgc ggttggggtt gatcgccgcc aactccgcgg
     4681 cgagggcgcc acccagcgag tgaccgacga ccgccgtgct gccgaggccg agtgcatcga
     4741 gcacatcgtt gagataggtg agcaggtcga gcatgtcgtc gagccgctca agggtggcct
     4801 cctcaccggc cacctggggg gcgtagacgg tgaacgactg ggagagctgc tcgaggtagt
     4861 cgttccaccg caggccgtac tggctgtgca ggtaaaccaa cgcgggcccg gtgcccttga
     4921 cgatgacgtc ggtctggaac ggttcggcga ggaccgtacg cgtgtcggtg ggggtgtcca
     4981 ctgcagtcat ggtgaggatc cttacttcgc gttgcggcgc gccgcttcga tgctggcggg
     5041 gctcttcttc ttggtgcccg agggccacca cttgtcttcc cactcgtcct cgaacatcga
     5101 ctgcaggcgc ggcaggtnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5221 nnnnnnnnnn nnnnnnnnnn nnnnnggtgc cggcgacgac ccatccgtcg ttgaggatct
     5281 tcgaccactt cttgttgtcc ggggtcgcct ggtactccat gatcgacttc atcagtcgtt
     5341 ctgaggcgcc cgacttcttc atcgcccgat cggtcttgta gccgggcgga tcgaagtagt
     5401 gcagcggcgt gccctgaacc agttggtaga agttgacgac gtgctcggcg aagtcgcgct
     5461 cgacggcagc gtcactctcg ccgacgcaga tcaacgacag gtagcccaac cggaacgggt
     5521 tggcgtcacg gcccagctcg tccaccgctt cccagtacat ctcgatggac ggacgggcga
     5581 actgggcacc gttgaacgtg aagaagacga acggccagtc gttgttgatc gcccgttccc
     5641 aggagtcgac cgactcgccg ccgagcagcc agaccggcgg atgcggacgc tggtacggca
     5701 gcggctccgg cgagacgtaa cgcagcttgt tgaaccggcc gttgtagacg aacggtccct
     5761 cctgggtcca cgacttcaag atcagatcga tcgcctcgct gtagcgcggg cgggtcttcg
     5821 aggccgcgat gccattcgta aagttcaagt ccatcgagat gcccaccggg aaggccgaaa
     5881 caacacggcc ctccgacagg atgtcgagca tcgcgatctc ctccgcgacg cgcaacggct
     5941 ccgcgatggc gtccccgatc gtctgcgagg gatcgagcga actgaacggg tcctggaaca
     6001 cggcctgcag ggtcgaacgc agggcgccgc ggcgccgcgg ggtgaggtga
//
LOCUS       DAORNF010000106         6026 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_9736_length_6026_cov_1.493552, whole genome shotgun sequence.
ACCESSION   DAORNF010000106 DAORNF010000000
VERSION     DAORNF010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 6026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6026
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9736_length_6026_cov_1.493552"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="PLV68_05750"
     CDS             <1..333
                     /locus_tag="PLV68_05750"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HRE00782.1"
                     /translation="WLFLSGLILTTRISARTERAAEFEADRRAVAMGFGRELATALRR
                     VEHDASLRDGGVTRLRGELHQSHPQPRARVQRIEAMLRRDRTGRDGTGRDGTRRNGTR
                     RGGTRRGR"
     gene            complement(334..2715)
                     /locus_tag="PLV68_05755"
     CDS             complement(334..2715)
                     /locus_tag="PLV68_05755"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HRE00783.1"
                     /translation="MYIRIARWCFKHRVIVVVAWAIAFVVIQAVSSAVGTDFSQEFDS
                     LGSESDRGFAVLREDFGGDGASYLKGTIVVRAEQGIDTPHVRGAVDQLLTEARGVEGI
                     GVVGPFDAQANGQVSADGTIAFATLEGNEDLVTFNDTAALGKDLLDLGNNLETQIPGL
                     QVEIGGQAFSQFEVPESEVIGIAFAIVILILAFGSVLAMGLPMAVALAGVGIGIALSN
                     LASNITAMPEMAPIIATMIGLGVGIDYALFVVTRYREGLHDGFPPEAATAAAGDTATR
                     AVVFAGLTVVVSLLGLLLIGLSFVAGLGVAAAITVAVTIAATVTLLPALLGFAKLRVE
                     LTRWRGLLAAGLVAVALLGVGLKVDVLLFAAPLGVLVLIAGLFVPVLKRPVKMPARKP
                     VEQSIWYRWSHVIQRHPWKSLLAGVAILLLCAFPLLDLRLASTDEGNFPDDSTTRQAY
                     DLLAEGFGPGFNSPLIVVAKVGSEADLSAFTSVVAAVKADPGVIDDGLPPFPNNPDNP
                     TAAIAQVYSRTSPQSVETAELVERLRNDVVPAAEAATGGQVEAFVTGLVPADIDFSDY
                     MSGRQLIFFGVVLAASFILLMIVFRSVLVPVKAVIMNLLSIAGAYGAVVALFQWGHFG
                     SITGIAEGPIEPWAPMMLFAIVFGLSMDYEVFLLSRIREEYEHTGDAKNSVADGLAST
                     ARVITAAAAIMVVVFGSFILEDNRVSKLMGTGLAMAVLLDATVVRMLLVPATMELLGH
                     KNWWIPKWLDRILPKLHVEGAPTHTDDIARIVAETEAETASAAESELEPIGAR"
     gene            complement(2929..>3288)
                     /locus_tag="PLV68_05760"
     CDS             complement(2929..>3288)
                     /locus_tag="PLV68_05760"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HRE00784.1"
                     /translation="GGPGMREMLAITGAMKGAGRGSDCALVTDGRFSGGTWGFCIGHV
                     APEAVDGGPIAFVRDGDVIRVDVHNYALDLLVDEAELERRRAELRQPLAPRYTSGVLG
                     KYAKLVKGAETGAITNI"
     assembly_gap    3289..3544
                     /estimated_length=256
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3545..>3760)
                     /locus_tag="PLV68_05765"
     CDS             complement(<3545..>3760)
                     /locus_tag="PLV68_05765"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HRE00785.1"
                     /translation="DLHLDDFNRIAAKVPHIADMKPGGRFHMSDLDRVGGVPVVMKHL
                     LDAGLLHGDCLTVTGKTVAENLAELNPP"
     assembly_gap    3761..3947
                     /estimated_length=187
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3948..>4200)
                     /locus_tag="PLV68_05770"
     CDS             complement(<3948..>4200)
                     /locus_tag="PLV68_05770"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HRE00786.1"
                     /translation="VYNGSIMPGHLNGEALDITSVFEAIGACAAGKIDEEQLHDIEIN
                     ACPGEGACGGMFTANTMSSIAEVIGMSLPGAAAPPAIDGR"
     assembly_gap    4201..4329
                     /estimated_length=129
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4330..4651)
                     /locus_tag="PLV68_05775"
     CDS             complement(<4330..4651)
                     /locus_tag="PLV68_05775"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HRE00787.1"
                     /translation="MSESEPTSATPVDIKPRSRDVTDGMQKAASRAMLRAVGMGESDW
                     VKPQVAIANSWNEVTPCNLTLRKLAEKVKDGVRAAGGFPMEFGTITVSDGISMGHEGM
                     RASLV"
     assembly_gap    4900..4928
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4964..>5245
                     /locus_tag="PLV68_05780"
     CDS             4964..>5245
                     /locus_tag="PLV68_05780"
                     /inference="COORDINATES: protein motif:HMM:NF015999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HRE00788.1"
                     /translation="MAIVPTSNRLSLKEMATAVGGKRAEMCDPAVAERVTGYVVGGIS
                     PFGQKRGLPTVIDETAELFGTIFVSGGKRGLDLELAPADLITVLTATVAP"
     assembly_gap    5246..5318
                     /estimated_length=73
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5319..>5653)
                     /locus_tag="PLV68_05785"
     CDS             complement(<5319..>5653)
                     /locus_tag="PLV68_05785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HRE00789.1"
                     /translation="VAHRDWDDYELGRASAIAHLGALDLVYRGVIESHREAIDIVGDI
                     DPVTEDMLIGQTAELEMYHWFVRSHLADYAGGLANAGSDTEIGAAQSAVRKVSGRRTG
                     AADTSRAGS"
     assembly_gap    5654..5663
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5664..5983)
                     /locus_tag="PLV68_05790"
     CDS             complement(<5664..5983)
                     /locus_tag="PLV68_05790"
                     /inference="COORDINATES: protein motif:HMM:NF012436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HRE00790.1"
                     /translation="MATSTRSRPASSHRSSTGGVSSTRNASFTAPGLTERDGATVITA
                     LQGRLGALIDLGLTLKHIHWNVVGPTFIGVHTMLDPQYAGVQAQVDATAERIATLGGV
                     PNGLP"
BASE COUNT          882 a         1779 c         1844 g          837 t
ORIGIN      
        1 tggctcttct tgtccgggct gatcctgacg acgcgcatct cggcccgtac cgagcgtgcc
       61 gccgagttcg aagccgaccg ccgggcggtc gcgatgggct tcggtcgcga gttggccacg
      121 gccttgcgcc gtgtcgagca cgacgcgtcg ctccgggatg gcggcgtgac gcgactgcgc
      181 ggtgagctgc accagtcgca cccccagccg cgggcccgcg tgcagcgcat cgaggcgatg
      241 ctccgtcgcg accggaccgg gcgtgacggg accgggcgtg acgggaccag gcgtaacggg
      301 acgagacgcg gcgggacgag acgcggcaga tagtcagcgg gcgccgatcg gttcgagctc
      361 ggactcggct gccgatgcgg tctcggcttc ggtctcggcg acgatgcggg cgatgtcgtc
      421 cgtgtgggtc ggcgccccct cgacgtgcag cttgggcaag atgcggtcga gccacttggg
      481 gatccaccag ttcttgtggc cgagcagctc catggtggcc ggcaccagca acatgcgcac
      541 gacggtcgcg tcgagcagca ccgccatcgc cagaccggtg cccatcagct tgctgactcg
      601 gttgtcctcc aggatgaagc tgccgaagac gacgaccatg atcgccgctg ccgccgtgat
      661 gacccgcgcg gtcgaggcga gaccgtcggc caccgagttc ttggcgtcgc cggtgtgctc
      721 gtactcctca cggatgcgcg agagcaggaa cacctcgtag tccatcgaca atccgaacac
      781 gatcgcgaac agcatcatcg gtgcccacgg ttcgatcgga ccctcggcga tgccggtgat
      841 cgagccgaag tggccccact ggaacagggc cacgaccgcg ccgtaggcgc cagcgatcga
      901 cagtaggttc atgatgacgg ccttgacggg tacgagcacc gagcggaaca cgatcatcaa
      961 caggatgaac gaggccgcca gtacgacccc gaagaagatc agttggcgcc ccgacatgta
     1021 gtcactgaag tcgatgtcgg ccggcaccag accggtgacg aacgcttcga cctggccacc
     1081 tgtcgcggct tcggcggcgg gcaccacgtc gttgcgcaag cgctcgacga gttcggcggt
     1141 ctcgaccgac tgcggagacg tccgcgagta cacctgcgcg atcgcggcgg tggggttgtc
     1201 ggggttgttg ggaaatggcg gtagcccgtc gtcgatcaca cccgggtcgg ccttgacggc
     1261 ggcgacgacg gacgtgaagg cgctcaggtc ggcctcgctg ccgaccttgg ccaccacgat
     1321 caacgggctg ttgaagccgg gtccgaaccc ttcggcgagc agatcgtagg cctggcgggt
     1381 ggtcgagtcg tcagggaagt tgccctcgtc ggtcgaggcc aggcgcaggt cgagcagggg
     1441 gaacgcgcac agcagcaaga tcgcgacacc ggcgagcagc gacttccacg ggtggcgctg
     1501 gatgacatgg ctccagcggt accagatgct ctgctcgacc ggcttgcggg ccggcatctt
     1561 gaccggacgc ttcaacaccg gcacgaacag gccggcgatc agcaccagca ccccgagcgg
     1621 agcggcgaac aacagcacgt cgaccttgag ccccacgccc aggagtgcga ccgcgaccag
     1681 gccggcggcg agcaggccgc gccaacgggt gagttcgacg cgcagcttgg cgaatccgag
     1741 cagcgccggg agcagggtga cggtggcggc gatggtgacg gccacggtga tcgccgcagc
     1801 gacacccaag ccggccacga acgacaggcc gatcaacaac aggcccagca gcgagaccac
     1861 cacggtgagc ccggcgaaca cgacagcgcg ggtggccgtg tcgcccgccg cagcggtggc
     1921 cgcttcgggc gggaacccgt cgtgcaggcc ttcgcgatag cgggtgacga cgaacaaggc
     1981 gtagtcgatg cccacaccga ggccgatcat ggtggcgatg atcggcgcca tctctggcat
     2041 cgccgtgatg ttcgacgcca ggttcgacaa ggcgatgccg atgcccacac ccgccaacgc
     2101 gaccgccatc ggcaggccca tggccagcac cgacccgaag gcgaggatga ggatgacgat
     2161 cgcgaacgcg atgccgatca cctcggactc gggcacttcg aactggctga aggcctggcc
     2221 gccgatttcg acctgaaggc cggggatctg cgtttccagg ttgttgccca ggtcgagcaa
     2281 gtccttgccc agcgcagcgg tgtcgttgaa ggtgacaagg tcctcgttgc cctccaacgt
     2341 ggcgaacgcg atcgtgccgt cggccgatac ctggccgttc gcctgcgcgt cgaacggccc
     2401 gacgacaccg atcccttcga caccgcgtgc ctcggtgagc aactggtcga ctgctccacg
     2461 aacgtgcggt gtgtcgatgc cctgttcggc cctgaccacg atcgtgccct tgaggtagga
     2521 cgcgccgtca ccaccgaagt cctcacgcag caccgcgaag ccgcggtcgg actcggagcc
     2581 gagcgagtcg aactcctggc tgaagtcggt gcccaccgcg gagctgaccg cctggatgac
     2641 gacgaacgcg attgcccagg cgacgacgac gatgacacgg tgcttgaagc accagcgcgc
     2701 aatgcggatg tacacggtaa aaggccctct cgatggggtt ctgctcgaac gatggggttc
     2761 ggcgcgccgc gagcgtgggg attcgtgacc ggagcgccgg ggtcgggaat gcacgctaac
     2821 gacggcgccc gccgccgtca cgggggatat cccccctctt cgttcagacg tcccgctcgg
     2881 ctgccgagct cagatatcga gatcagatac cgggctcaga taccgggctc agatattcgt
     2941 gatcgcgccg gtctcggcgc ctttgaccag cttggcgtac ttgcccaaca caccgctggt
     3001 gtagcgcggc gccagcggtt gccgcaactc cgcacgacgc ctctccagtt cggcctcgtc
     3061 gaccagcagg tcgagcgcat agttgtgcac gtcgacgcgg atgacgtcgc cgtcgcggac
     3121 gaacgcgatc ggcccgccgt cgaccgcttc gggggcgaca tggccgatgc agaatcccca
     3181 cgtaccgccg ctgaagcgac cgtcggtgac cagggcgcag tcgctgcccc ggcccgcacc
     3241 cttcatcgct ccggtgatcg cgagcatctc gcgcatgccc gggccgccnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnggcggg ttgagctcgg ccaggttctc ggccacggtc ttgcccgtga cggtcaggca
     3601 gtcgccgtgc agcaggcccg cgtcgagcag gtgcttcatc accaccggca caccgccgac
     3661 ccggtcgagg tcgctcatgt ggaaccgccc gccgggcttc atgtcggcga tgtggggcac
     3721 cttggcggcg atgcggttga agtcgtcgag atgcaggtcg nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnggc gtccgtcgat
     3961 ggcgggcggg gcagccgcgc ccggcaggct catgccgatc acctcggcga tcgacgacat
     4021 ggtgttggcg gtgaacatgc caccgcacgc gccttcgccc gggcaggcgt tgatctcgat
     4081 gtcgtgcagc tgctcttcgt cgatcttgcc ggccgcgcag gcgccgatcg cttcgaacac
     4141 gctggtgatg tcgagcgctt caccgttgag gtgacccggc atgatgctgc cgttgtagac
     4201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnnnnt gaccagcgag gcacgcatgc cttcgtggcc catgctgatg ccgtcgctga
     4381 cggtgatggt gccgaactcc atcgggaacc cacccgcggc gcgcacgccg tctttcacct
     4441 tctcggccag cttgcgcagg gtgaggttgc acggtgtcac ctcgttccac gagttggcga
     4501 tggccacctg cggcttcacc cagtcgctct cgcccatacc gaccgcgcgc agcatggccc
     4561 gcgacgcggc cttctgcatg ccgtcggtga cgtcacggct gcgcggcttg atgtcgaccg
     4621 gcgtcgcgga tgtgggctcg ctctcgctca tggcgccaac gctaccgatg ccgacgtgca
     4681 cggtctgtat cgataggtcg ctgtcggtgt gttgcgcgcg aaagtgaggg cgtggtgcgc
     4741 gatgcaggac acaagacggc ggtggcaggc ggcaagggaa cgccggccat ggccgcgctg
     4801 accgccgcag gcgtcacgtt cgtggcccac accttcgccc acgacgcggg tgctaccaac
     4861 tacgggttgg aggcggcagc caacctcggt gtcgagccan nnnnnnnnnn nnnnnnnnnn
     4921 nnnnnnnntg gctatcgacg ggctgcccaa gccagggctt gccgtggcga tcgtgcccac
     4981 cagcaaccgg ttgtcgttga aggagatggc cacggccgtc ggcggaaaac gggccgagat
     5041 gtgcgaccca gcggtcgccg agcgcgtcac cggatacgtc gtcggcggca tcagcccgtt
     5101 cggccagaag cgggggttac cgaccgtgat cgacgagacg gccgaactgt tcggcaccat
     5161 cttcgtcagc ggcggcaagc gcggcctcga cctggagctc gcccccgccg acctcatcac
     5221 ggtgttgaca gcgaccgtcg cccccnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntg ctcccggctc ggctggtgtc
     5341 ggccgcgccc gtgcgtcggc cactgacctt gcgcacggcc gattgggccg ctccgatctc
     5401 ggtgtctgac ccggcgttgg ccaagccgcc tgcgtagtcg gcgaggtgcg agcggacgaa
     5461 ccagtggtac atctccaact ccgcggtttg gccgatcagc atgtcctcgg tgaccgggtc
     5521 gatgtcgccg acgatgtcga ttgcctcgcg gtgcgactcg atcacgccgc ggtagaccag
     5581 atcgagcgcg cccaggtggg cgatcgcgct ggcccggccc aactcgtagt cgtcccagtc
     5641 gcggtgcgcg accnnnnnnn nnngggaggc cgttcggcac tccgccgagg gtggcgatcc
     5701 gttccgctgt cgcgtccacc tgagcctgga ccccggcgta ctggggatcg agcatcgtgt
     5761 gcacgccgat gaaggtcggc ccgacgacgt tccagtggat gtgcttcaag gtcagaccga
     5821 ggtcgatcaa cgcaccgagg cgaccttgca gcgcggtgat taccgttgcg ccgtcgcgct
     5881 cggtgagccc gggtgcggtg aacgaggcgt tgcgggtgga actgacgcca ccggtcgatg
     5941 aacggtgtga cgaagcagga cgcgagcgag tcgaagtagc catgccggat cgatacccca
     6001 cgcgtcaacc gctcaaacca gcctgg
//
LOCUS       DAORNF010000107         6006 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_9782_length_6006_cov_1.854814, whole genome shotgun sequence.
ACCESSION   DAORNF010000107 DAORNF010000000
VERSION     DAORNF010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 6006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6006
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9782_length_6006_cov_1.854814"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>780
                     /locus_tag="PLV68_05795"
     CDS             <1..>780
                     /locus_tag="PLV68_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00791.1"
                     /translation="VLLERMWPVLTPADLLHDLFGSKALLKLAGQRHLREHEYLALYR
                     PRSADVHDVAWSPADVALLDEARHYLGVRPSKGQADDADEIRTYGHIVIDEVQDLTPM
                     QLIMVARRSLNGSMTVVGDIAQATGPLAPNSWNDLLAYLPDRKGSRVIGLSVGYRIPA
                     QIMELANRVMAVATPELKPPTAVREGDVGPDIMQVDADDLEATVAAETARLIDEVPGG
                     NLAVVAPDAMVDDLSKALANAGVTHGRATRTGLESGVTVVAV"
     assembly_gap    781..900
                     /estimated_length=120
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1031..1468)
                     /locus_tag="PLV68_05800"
     CDS             complement(1031..1468)
                     /locus_tag="PLV68_05800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00792.1"
                     /translation="MIQPIRLAAARRAIAALAGVGICLTAAGCATTRLDRSSATTAVP
                     TTTTLPDGGATELLPLLVGEASQLSNVIGANGDRSEQMTRIQNVYDAVRPDIDQTNGL
                     LADSFDAAITLCQRATQFKRPADADKCFRNLEALSDQYLTAIG"
     gene            complement(1497..2159)
                     /locus_tag="PLV68_05805"
     CDS             complement(1497..2159)
                     /locus_tag="PLV68_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HRE00793.1"
                     /translation="MSRSGEHHPAFEEYCEAIFELQEDDVEVIQARIVERLNVSRPAV
                     SEMIRRLESEKLISVADAKITLTKAGNSLAQQVVRRHRLAERFITDVLGLSWAEAHHE
                     AGKWEHVMSTNVEMAMDRLLGSPTTCPHGNPIPGSSYSPPDARRLVDLGVGDSFIVSR
                     IPEELEFTPGLLDFLENASLLPGHTGTVTASSPDGTMTVEVEGSHVGVGAFASARILV
                     TV"
     gene            complement(2250..3929)
                     /locus_tag="PLV68_05810"
     CDS             complement(2250..3929)
                     /locus_tag="PLV68_05810"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HRE00794.1"
                     /translation="MRLRPWQKSAFDKFSAIGEEDSPNFLAVATPGAGKTTFALTCAR
                     WSLAAERRRLIVVAPTSHLKVQWSMAAHRLGLQLDPDWTPAGGVAKDVHGIVTTFQQV
                     ATGSTAKVLAGVSDGAFVILDELHHAGDEKAWGDAVRIAFAKAHRRLCLSGTPFRSDT
                     ASIPFVHYDDDQAVADYEYGYGDALRDGGVVRPVYFPRVDGHMEWSAPDGSVLSASFQ
                     DELTRDQVSQRLRAALSLDGEWLPHVIEQGHERLMHVRRTHPDAGGLVIATDQEHAHG
                     IARLIRSRFGVAADVVVSDDPGASKRIAAFATNDEPWLVSVRMVSEGVDIPRLRVGVY
                     ATTVSTERFVRWQAGHASQRAFVYLPDDVRLRTHAFQIMESRRHVLRRAHAEDDNDVI
                     AGIPEHDAPFDEVPQEMLVEQLSLFSVLSAVATGIEIHAIGSDGIAHPGAVDIATLDT
                     IGDDEDEIDASAASDDPLLELSLPDIPTDAGWAHERGRSVFEIKDELRDKNAATAKRL
                     VDLTGWTHARVNGEMNRRAKVTTVSNATIDELDRRLRHADEWLDQLRAGRR"
     assembly_gap    4189..4272
                     /estimated_length=84
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4365..>6006
                     /locus_tag="PLV68_05815"
     CDS             4365..>6006
                     /locus_tag="PLV68_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00795.1"
                     /translation="MHSASGSNVAVTAKNGKEVTGIDEDEAKTMYVGTFTNGSGTLDA
                     VTGAGTISWETDKWQMDPYPTVPGPHIIQLEDPQLDIAADGSGALSFKVTLLKDSDLT
                     VEPGKGEVRQTMATFADVSELGVNGGTLTPDFAGRPYAGYEECDGTGGSWPAGWIDFL
                     PSPMPNLKAFFYTTTSGGGCTAAGQVRKPALPVHFAWNASVDSSIVALTPARILDSRA
                     DGVTVDGDFAKGGALVGGTQLTLDVAGRGGVPDNAKVAVLNVTATEAEDAAFVTVWPC
                     DAVRPTASSLNVPKGGTVPNMVVATLAADGTACLFSNVTTHLIADVTGYISTAVGYTA
                     LTPARLADTRADGITVDGDAVNTGITTVGGVVRVPVEGRGDVPAAVSTAVLNVTVTEP
                     VEGGFVTVWDCDATRPLASSVNYAKGQTVANTVFSSLSSAGEVCLFTSNPTHLVVDIN
                     GAFTAGASLAPLTPARLVDTRDAGQTVDGDQQGGGRTAVGATFAVDVADRGGVPADAR
                     SVVVSIVATDAVAGGFVTVYDCGQTVPNASAVNYTAGSN"
BASE COUNT          902 a         1891 c         2048 g          961 t
ORIGIN      
        1 ggtgctgctc gaacggatgt ggccggtgct caccccggcc gatctgctcc acgacctgtt
       61 cgggtcgaag gcgctgctga agctggccgg ccagcgtcac ctgcgtgagc acgaatatct
      121 cgcgctgtat cgtccccgct cggccgacgt gcacgacgtg gcctggagcc ctgccgacgt
      181 cgccctgctc gacgaggctc gccactacct cggcgtcagg ccgtccaagg gacaagccga
      241 cgacgccgac gagatccgta cctatggcca catcgtgatc gacgaggtgc aggatctcac
      301 gccgatgcag ttgatcatgg tggcccgccg ctcgctgaac gggtcgatga ccgtggtcgg
      361 cgacatcgcc caggcaaccg gtccgctggc gcccaactcg tggaacgacc tgctcgcgta
      421 cctgcccgac cggaagggca gccgggtcat cgggctcagc gtcggctacc gcatcccggc
      481 ccagatcatg gagctggcca accgcgtgat ggcggtggcg acgcccgagc tgaagccgcc
      541 caccgcggtg cgggagggcg acgtcggccc cgacatcatg caggtcgatg ccgacgatct
      601 cgaagccacg gtcgcggccg agacggcccg tctgatcgac gaggtgcccg gtggcaacct
      661 ggcggtggtc gcgcccgatg cgatggtcga cgatctgtcc aaggcgttgg ccaacgcggg
      721 cgtcacccac ggccgggcca cgcgcaccgg gctggagtca ggtgtgaccg tggtggcggt
      781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      901 gtggtgcacg ccaagccgct gcccgccgcg atgcggtgag gcggcccagc tcgctgtcac
      961 tgcagcggct gcggcctccg cagtcctcgc cgtgtcgagg ccccggccga tcacggtcgg
     1021 ccgatcacgg tcagccgatc gcggtcaggt attggtcgct gagggcttcg aggttgcgga
     1081 agcacttgtc cgcgtcggca gggcgtttga actgcgtggc ccgctgacag agggtgatcg
     1141 cggcatcgaa gctgtcggcc agcaggccgt tcgtctgatc gatgtcggga cgcacggcgt
     1201 cgtacacgtt ctggatccga gtcatctgct cgctccgatc accattggca ccgatgacgt
     1261 tcgacagttg gctcgcctcg ccgaccagca gcggcagcaa ttcggtggca ccgccgtcgg
     1321 gcaacgtggt ggtggtcggt accgcggtcg ttgccgacga gcgatcgagc cgggtggtgg
     1381 cgcaaccggc tgcggtgagg cagatgccga cgcccgccag tgcggcgatg gcgcggcggg
     1441 cagcggcgag tcggatcggt tggatcatgg cgtgggtgag gagggtggag tgctgatcag
     1501 acggtgacga ggatgcgggc gctggcgaag gcgccgacgc ccacgtgcga accctcgacc
     1561 tcgacggtca tggtgccgtc gggcgacgac gcggtgacgg tgcccgtgtg acccggcagc
     1621 agcgaggcgt tttcgaggaa gtcgagcagc ccgggggtga actcgagctc ttcggggatg
     1681 cggctgacga tgaacgagtc accgacgccg agatcgacca ggcgccgcgc atcgggtggc
     1741 gagtagctgc tgcccgggat ggggttgcca tgagggcagg tggtggggct gccgagcagg
     1801 cggtccatcg ccatctcgac gttggtgctc atgacgtgct cccacttgcc cgcctcgtgg
     1861 tgggcttcgg cccaggacaa cccgaggacg tcggtgatga accgctcggc cagccggtgc
     1921 cgacggacga cttgttgggc cagcgagttg cccgccttgg tcagcgtgat cttggcgtct
     1981 gcgaccgaga tcagcttttc gctttcgaga cggcggatca tctcagacac ggccggacgg
     2041 ctgacgttga gccgttcgac gatgcgagcc tggatgactt cgacgtcgtc ttcctgaagt
     2101 tcgaagattg cttcgcaata ctcttcgaac gcgggatggt gttcacctga gcgcgacatg
     2161 tcatgactct atcgaatggt tccggagcga ggtccggatc tggcgtggcg agggccacgc
     2221 caccaggtct ggttgcggag gttggcgtgt cagcggcgac cggcccggag ctggtcgagc
     2281 cactcgtcgg cgtgacgcag gcgccgatcg agctcgtcga tcgttgcatt cgaaacggtc
     2341 gtgaccttgg cgcggcggtt catctcgcca ttgaccctgg cgtgggtcca gccggtcagg
     2401 tcgaccagcc gcttggcagt cgcggcgttc ttgtcgcgca gctcgtcctt gatctcgaac
     2461 acgctgcgac cgcgctcgtg cgcccatccc gcgtcggtgg gaatgtcggg caggctgagc
     2521 tccagcagcg gatcgtccga cgccgctgac gcgtcgatct cgtcctcgtc gtcgccgatc
     2581 gtgtcgagcg tggcgatgtc gactgctccc gggtgggcga tgccgtcgct gccgatcgcg
     2641 tggatctcga tgccggtcgc caccgcggac agcacgctga acagcgacag ctgctcgacg
     2701 agcatctcct ggggcacctc gtcgaacggt gcgtcgtgct cggggatgcc ggcgatcaca
     2761 tcgttgtcgt cctcggcgtg ggcccggcgc aacacatggc ggcgcgactc catgatctgg
     2821 aacgcgtggg tgcgcaggcg cacgtcgtcg ggcaggtaga cgaaggcccg ttgcgatgcg
     2881 tgccccgctt gccagcgtac gaagcgctcg gtcgacacgg tcgtcgcgta gaccccgacc
     2941 cgcaggcggg ggatgtcgac gccctcggac accatccgca ccgacaccag ccacggctcg
     3001 tcgttggtgg cgaaggcggc gatgcgtttc gacgcgcccg ggtcgtcgct gacgacgacg
     3061 tccgccgcca ccccgaaccg cgaccggatc aggcgggcga tgccgtgggc gtgctcttga
     3121 tcggtggcga tgacgagccc accggcatcc gggtgggtgc ggcgaacgtg catcagccgt
     3181 tcgtggcctt gctcgatcac gtgggggagc cattcgccgt cgaggctcag cgcggcgcgc
     3241 aaccgttggc tgacctggtc gcgggtgagc tcgtcctgga acgacgccga cagcaccgat
     3301 ccgtcgggcg cgctccactc catgtggccg tcgacgcggg ggaagtacac cgggcgtacc
     3361 acaccgccat cgcgcagcgc gtcgccgtag ccgtactcgt agtcggccac cgcctggtcg
     3421 tcgtcgtagt ggacgaacgg gatcgacgcg gtgtcgctgc ggaacggcgt accgctgagg
     3481 cacaggcggc ggtgggcctt ggcgaacgca atccgcaccg cgtcgcccca cgccttctcg
     3541 tcgccggcgt ggtgcaactc gtcgagaatc acgaaggcgc cgtcggacac gccggccaga
     3601 accttggcgg tgctgccggt ggccacctgc tgaaacgtcg tgacgatgcc gtggacgtcc
     3661 ttcgcgacgc cgccggcggg ggtccagtcg gggtcgagct ggagacccaa gcggtgggcg
     3721 gccatgctcc actgcacttt gaggtggctg gtcggggcga cgacgatcag ccgccgacgc
     3781 tcggcggcca acgaccaccg ggcgcaggtg agggcgaagg tggtcttgcc ggcaccgggc
     3841 gtggcgacgg cgagaaaatt cggggagtcc tcctcgccga tggcgctgaa cttgtcgaag
     3901 gcggacttct gccacggccg aaggcgcact cccgtcatcg tggcgccgag tctggcgggc
     3961 cgggcgcccg gccccaactc gacgacgctg ccctgtgact tttgacgaca acttgttgcc
     4021 aaacctgacg ggtttgtgct agatacacca ctttccgtac tgtgttaagt agtccgtact
     4081 cacgatcaac agcacagcga ctagagagac agttcccaga gagatggaga ccagcatggg
     4141 taacgacgca atgatcgccg acgcctcccg gtggcgatcg aagagacgnn nnnnnnnnnn
     4201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4261 nnnnnnnnnn nncggcgcca ttgccaccca cggtgaccgt caccggcgcc tcgttggaat
     4321 gggctttcag tcgcgtcggc gcatgggcat cgccagtggc atgggtgcat tcggcctcgg
     4381 gatcgaacgt tgccgtcacg gccaagaacg gcaaggaggt caccggtatc gatgaggacg
     4441 aggccaagac gatgtacgtc ggcacgttca ccaacggctc cggcaccctc gacgccgtga
     4501 ccggggcagg cacgatctcg tgggagaccg acaagtggca gatggacccc taccccacgg
     4561 tgcccgggcc gcacatcatc caactcgaag acccgcaact ggacatcgcc gccgatggca
     4621 gcggtgcgct gagcttcaag gtgacgttgc tcaaggactc cgatctcacg gtcgagccgg
     4681 gcaagggcga ggtgcgccag acgatggcga ctttcgccga cgtcagcgaa ctgggagtca
     4741 acggcggcac gctcacgccc gacttcgccg gtcggcccta cgcgggttac gaggagtgtg
     4801 acggcaccgg tggctcctgg ccagcgggtt ggatcgactt cttgccgtca ccgatgccga
     4861 acctgaaggc cttcttctac accacgacca gcggtggtgg ttgcaccgcc gccggtcagg
     4921 tgcgcaagcc ggcgctgccg gtgcacttcg cgtggaacgc ctccgtcgac tcgagcatcg
     4981 tcgcgctgac gcccgcgcgc atcttggaca gtcgggccga tggggtgacc gtcgacggag
     5041 acttcgccaa gggtggcgcg ctggtcggcg gcacgcagtt gacgctcgat gtcgcaggcc
     5101 gcggcggtgt gccggacaat gccaaggtgg ccgtgctcaa tgtgaccgcg accgaggccg
     5161 aggatgccgc tttcgtcacg gtgtggccgt gcgacgccgt tcgcccgacc gcgtcgagcc
     5221 tcaacgtgcc caagggtggc acggtgccga acatggtcgt ggccacgctg gccgccgacg
     5281 gcacagcgtg cttgttctcg aacgtgacca cgcacctgat cgccgacgtc accggctaca
     5341 tctcgaccgc ggtcggctac accgcgttga cccctgctcg tctggccgac acccgcgctg
     5401 acggcatcac cgttgacggc gatgcggtga acaccggcat caccacggtt ggtggggtcg
     5461 tacgcgttcc ggtcgaaggt cgcggcgacg tacctgctgc agtcagcacg gccgtgctca
     5521 acgtcacggt caccgagccg gtggagggtg gcttcgtcac cgtgtgggac tgcgacgcaa
     5581 cgcgaccgtt ggcctccagc gtcaactacg ccaagggcca gacggtggcc aacacggtgt
     5641 tctccagctt gtcgagcgcg ggtgaggtgt gcctgttcac atcgaacccg acccatctgg
     5701 tggtcgacat caacggtgcg ttcactgccg gtgcgtcgct ggcgccgctc accccggccc
     5761 gtctggtcga tacacgtgac gccgggcaga ccgtcgatgg cgaccagcaa ggcggtggcc
     5821 gtacggccgt cggcgcgacc ttcgcggtcg acgtggccga ccgcggcggt gtgcccgccg
     5881 atgcccggtc cgtcgtggtg agcatcgtcg cgaccgacgc ggtcgcgggc ggcttcgtca
     5941 cggtgtacga ctgcggccag accgtgccca acgcttcggc ggtcaactac accgctggca
     6001 gcaact
//
LOCUS       DAORNF010000108         6006 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_9783_length_6006_cov_1.477567, whole genome shotgun sequence.
ACCESSION   DAORNF010000108 DAORNF010000000
VERSION     DAORNF010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 6006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6006
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9783_length_6006_cov_1.477567"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1018)
                     /locus_tag="PLV68_05820"
     CDS             complement(<1..1018)
                     /locus_tag="PLV68_05820"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRE00796.1"
                     /translation="MTEPLRPVGVAPVDVGAGDAEPGAGVSTTAKDATAAAGILAASI
                     SPKKMALKRFLNHRAAVISTVVLAIVVLWVVLSPITARYGVNQAIFKSESGNLNSNLA
                     PRAAAWMGTDSNGRDIYSRMIFGARVSLLVGLFTALGSVLLGTAVGAIAGYKGGRIDD
                     LLMRATDIFLALPLLVILVIVRGALGNIGWITVIMGEKSSVRFLVILLSLIGWMGVAR
                     LVRGQVKQLREREFIEASRALGASTTRIMVRHLLPNSIGTIMVALTFSVVAAITAEAT
                     LSFFGFGPDPGAGMTSLGLLIRDSKKAVPPGYWWLVVYPGALFIVISLCINFIGDGLR
                     DALDP"
     gene            complement(1020..>1413)
                     /locus_tag="PLV68_05825"
     CDS             complement(1020..>1413)
                     /locus_tag="PLV68_05825"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRE00797.1"
                     /translation="IIVVSIQTIATYSRYMRSSLLDVVNSDYLRTARAKGISERRVLV
                     RHALRNALVPLVTLAALDIGAIVGGLIITEGVFAYPGMGQFFLTAESNGDFPELMPWM
                     VIITASVIIFNLLADLAYAWLDPRIKLD"
     assembly_gap    1414..1424
                     /estimated_length=11
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1425..1996)
                     /locus_tag="PLV68_05830"
     CDS             complement(<1425..1996)
                     /locus_tag="PLV68_05830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRE00798.1"
                     /translation="MLRYIVRRLLWAIPTLLLVTFVVYVALRLATNPVASYARANPRL
                     GKQPAKIKQYIEINGLWDGPGGYVRGYLHWLKGFVTGDWQPSIKGTRPVWPNLKDALA
                     NSLRLGFAAVAVGVIIGVSLGVFAGLRPGKLRDSAVNTFALVGFSVQPFISAVILQLV
                     FSVQWAKWFGSPLLPTSGVYPPGQRGFDLWL"
     gene            complement(2125..>3191)
                     /locus_tag="PLV68_05835"
     CDS             complement(2125..>3191)
                     /locus_tag="PLV68_05835"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRE00799.1"
                     /translation="QYSPETADAVAGDANVKVKIEFGGDYEGLYFQQQEGPFADPIYR
                     QAFAQSIDIEAFTAQIYAPIVADAEALTCGPIVPGPYCDESTFADTYDPEGAAKLLED
                     NGWVKDGDGYWSKDGTTPEVRWMVNTPNSRRENAQAYLIPLLLDAGFKVKADNGDADS
                     VFQQRLPNLDYDLGMYISTAPPDPQYVTSSFACDQIPSEENGFAGQNNVGWCNEDATE
                     KLYEADRTVDFDARAALVKEAIAAMRTDWVMLPTFQFPKSGLYRTDRVGGPVEADLNN
                     YQAFINLHEWTDVDGDGQIIIGAEQWPGCLNPITECANSSWYVWTVSFPLLMGPYDTT
                     NDGEYELTPLMAGEPVVELL"
     assembly_gap    3192..3359
                     /estimated_length=168
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3360..>3669)
                     /locus_tag="PLV68_05840"
     CDS             complement(<3360..>3669)
                     /locus_tag="PLV68_05840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00800.1"
                     /translation="AMRVTYNINDAAVWDDGSPVVADDFICTYDAVMNTVGSLSTVGY
                     DQISSIAAGDGDKQVVIEFKSIYAPYRGLFTPLIKNADIADCKDVSGDFQDSIPFSNR
                     P"
     assembly_gap    3670..3749
                     /estimated_length=80
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4142..4960)
                     /gene="tpiA"
                     /locus_tag="PLV68_05845"
     CDS             complement(4142..4960)
                     /gene="tpiA"
                     /locus_tag="PLV68_05845"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012231021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HRE00801.1"
                     /translation="MPPAEPHRARAASASSGRIPIISGNWKMHNNHFEAIQTVQKLSY
                     LVTKDDFAAVDVTIHPPFTDIRSIQTLIEADELLFGLGAQNCHWQDKGAFTGEISPVF
                     LAKLGVRYVICGHSERRELFAETDEDVNKKVHAIAQHGMTPIMCVGETLAEREAGGTE
                     DKVLGQVRAGLAGCAAAAVAEMVIAYEPIWAIGTGRTATADDAQAVCGAIRRCVGEIY
                     DQATASGVRIQYGGSVKAANIAELMSQPDVDGALVGGASLDPDEFARIVQFRNH"
     gene            complement(4947..>6006)
                     /locus_tag="PLV68_05850"
     CDS             complement(4947..>6006)
                     /locus_tag="PLV68_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HRE00802.1"
                     /translation="KRVLVRTDFNVPMDDGEITDDFRIRAALPTINWLTERGASVVTA
                     SHLGRPKGRPEPKYSMAPVRERLAELAPGVELMENLRFHPGEEGNDPAFVATLIDGID
                     AYVDDAFGAAHRAHASIVGPPQTLPSAMGLLLQREVEVLLGLRDHPKRPFVAVLGGAK
                     ISDKLGVVEALLNVVDSLIIGGAMCFTFFAPQGKPIGDSLFEPDQVDTCRRLLAGGKQ
                     IHLPEDITGVDASGAYATFGTRLPEGAKGFDIGPGSAAAFSDIVMDARTVFWNGPMGM
                     FEDERFAAGTRTLAQAMADTKAFTVVGGGDSAAALAQFGLDQVDHVSTGGGASLELLE
                     LGDLPGLAALRGAPNAPR"
BASE COUNT         1021 a         1866 c         1899 g          961 t
ORIGIN      
        1 gcgggtcgag ggcgtcgcgc aggccgtcgc cgatgaagtt gatgcacaac gagatcacga
       61 tgaacagcgc cccgggatac acgaccagcc accagtagcc gggcggaacc gccttcttcg
      121 agtcacggat caacagtccc agcgatgtca tgccagcgcc cggatcggga ccgaagccga
      181 aaaagctcaa cgtcgcctcg gcggtgatcg cggcgaccac cgagaacgtc agcgcgacca
      241 tgatcgtgcc gatcgagttc ggcaacaggt ggcggaccat gatccgcgtg gtcgacgcgc
      301 ccaacgcccg tgaggcctcg atgaactcgc gctcgcgcaa ctgtttgacc tggccgcgca
      361 ccaagcgggc gacccccatc cagccgatca acgagagcag gatcaccagg aatcgcaccg
      421 acgacttctc gcccatgatc acggtgatcc agccgatgtt gccgagcgcg ccacgcacga
      481 tgaccaagat caccagcagc ggcaaggcga gaaagatgtc ggtggctcgc atcagcagat
      541 cgtcgatccg cccgcccttg taaccggcga tcgcaccgac cgcggtgccg agcagcaccg
      601 agcccagcgc ggtgaacagg ccgaccagca gcgatacccg cgccccgaag atcatgcggc
      661 tgtagatgtc ccggccgttc gagtcggtgc ccatccacgc cgccgcgcgc ggggccaggt
      721 tggagttgag gttgccggac tcggacttga agatcgcttg gttgaccccg tagcgggcgg
      781 tgatcggcga gagcacgacc cacagcacga cgatcgccaa cacgaccgtg gagatcaccg
      841 cggcacggtg gttgaggaag cgcttgagcg ccatcttctt cggcgagatc gacgcggcaa
      901 ggatgccggc cgcggcggtc gcgtctttgg cggtggtgga gactcctgcg cccggttcgg
      961 catcgccggc accaacatcg acgggcgcga cacccacagg cctgagtggt tcggtcacgt
     1021 cagtcaagtt tgatcctcgg gtcgagccac gcgtaggcga ggtctgcgag caggttgaag
     1081 atgatgaccg aggcggtgat gatcaccatc cacggcatca gttcggggaa gtcgccgttg
     1141 ctctccgcgg tcaggaagaa ctgacccatg cccgggtagg cgaacacccc ctcggtgatg
     1201 atcaggccgc cgacgatcgc gccgatgtcg agcgcggcca atgtcaccaa cggcacgagc
     1261 gcattgcgca acgcgtggcg caccagcacc cggcgctcgc tgatgccctt cgcgcgagcc
     1321 gtgcgcagat agtcgctgtt gaccacgtcg agcaggctgg aacgcatgta gcggctgtag
     1381 gtggcgatgg tctggatgga tacgacgatg atcnnnnnnn nnnnagccac aggtcgaaac
     1441 ctcgttggcc aggcgggtag acgcccgagg tgggcagcag cggcgagccg aaccacttcg
     1501 cccactgcac cgagaacacg agctgcaaga tcacggcgct gatgaacggc tgcaccgaga
     1561 agccgacgag tgcaaaggtg ttgaccgcgc tgtcgcgcag cttgccgggt cgcaggccgg
     1621 cgaacacacc cagtgagacg ccgatgatga cgccgaccgc gaccgctgcg aatccgagac
     1681 gcagcgagtt ggccagggcg tctttgaggt tgggccacac cggacgggtg cccttgatgc
     1741 tgggctgcca gtctccggtg acgaagccct tgagccagtg cagatagccc ctgacgtagc
     1801 cgcccggccc atcccacaag ccgttgatct cgatgtactg cttgatcttg gctggttgct
     1861 tgcccaagcg cggattggcc cgcgcgtagc tcgccaccgg gttggtggca agacgcagtg
     1921 ccacgtacac gacgaacgtg acgagcagca gcgtcggtat tgcccacaac aggcgacgaa
     1981 cgatgtatct cagcaacggt ggttcccttc cactggctgg ctgtcgtgat gaaccggagt
     2041 tgaaccatgg ggccgaccgg atcgcttcgt gcgaagtcga tcggccggcc ccatgtctgt
     2101 caacgtgacg agtggtcgct caggtcagag cagttcgacc accggttcac ccgccatcag
     2161 cggagtcaac tcgtattcgc cgtcgttggt ggtgtcgtag ggacccatca acagcgggaa
     2221 gctcaccgtc cacacgtacc acgacgagtt ggcgcactcg gtgatcgggt tgaggcagcc
     2281 gggccactgc tcggcgccga tgatgatctg gccatcgccg tcgacatcgg tccactcgtg
     2341 gaggttgatg aacgcctggt agttgttcag gtcggcctcg accgggccac ccacgcggtc
     2401 ggtgcggtac aggcccgact tggggaactg gaaggtgggc agcatgaccc agtcggtccg
     2461 catcgccgcg atcgcttcct tgaccagggc ggcgcgggcg tcgaagtcga cggtgcggtc
     2521 ggcctcgtac agcttctcgg tcgcgtcctc gttgcaccag ccgacgttgt tctgaccggc
     2581 gaagccgttc tcttcggacg ggatctggtc gcaggcgaac gacgaggtga cgtactgcgg
     2641 atccggcgga gcggtcgaga tgtacatgcc caggtcgtag tcgaggtttg gcagacgctg
     2701 ctggaacacg ctgtcggcgt cgccgttgtc ggccttgacc ttgaaaccgg cgtcgagcag
     2761 cagcgggatg aggtacgcct gagcgttctc acgacggctg ttcggggtgt tgaccatcca
     2821 gcgcacttcc ggcgtggtgc cgtccttgct ccagtagccg tcgccgtcct tgacccagcc
     2881 gttgtcctcg agcagcttcg cggcaccttc gggatcgtag gtgtcggcaa aggtcgactc
     2941 gtcgcagtac gggccaggca cgatcggacc gcaggtcaac gcctcggcgt cagccacgat
     3001 cggagcgtag atctgggcgg tgaatgcttc gatgtcgatc gactgggcga aggcctgacg
     3061 gtagatcgga tcggcgaagg gaccttcctg ctgctggaag tacagaccct cgtagtcacc
     3121 gccgaactcg atcttgacct tcacgttcgc gtcgcccgcc accgcgtcgg cggtctccgg
     3181 gctgtactgc gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng
     3361 gacggttcga gaacggaatg ctgtcctgga agtcgccgga gacgtccttg cagtcggcga
     3421 tgtcggcgtt cttgatcaat ggagtgaaca ggccgcgata cggcgcgtag atcgacttga
     3481 actcgatcac gacctgcttg tcaccatcgc ccgctgcaat cgacgagatc tggtcgtagc
     3541 cgacggtgct cagcgagccg acggtgttca tgaccgcgtc gtaggtgcag atgaagtcgt
     3601 cggcgacgac cggcgagccg tcgtcccaca ccgctgcatc gttgatgttg taggtgacgc
     3661 gcatggccgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc gtcgtcgacg ccgacgtcga atcggtgccc
     3781 ggcgacgtgg tcgtcgtcgt gtccgccttc ttgtcgtcgc tcccgcaggc cgccgcgatc
     3841 agcgacagac cgacggcaac cgcagcaaac ttgctgcgct tcctggtgct cacgttgtaa
     3901 tgcctcctga ttggtctcgg gagatgtgcg gcaagccgcc atcggcgaag cccgggtcgc
     3961 cgaagactcc ccggacaaca cccccgtact gtcctccccg atcactacgg gtgcgaggac
     4021 catagcaccg ctcatcgggc ccaacgagta ctggcgttcc acgtaacgcg ctcgaaacga
     4081 acgattcttc cgcccgcgcg acagcggcga aacccgtggt gagacgggtg accggcggcg
     4141 gtcagtgatt gcggaactgg acgatgcggg cgaactcgtc gggatcgaga ctcgccccgc
     4201 cgaccaaggc accatcgacg tcgggctggc tcatcaactc ggcgatgttg gccgccttga
     4261 ccgaaccgcc gtactggatg cgcacgccag atgccgttgc ttggtcgtag atctcgccga
     4321 cacagcggcg gatcgcccca cacaccgcct gcgcgtcgtc ggcggtggcg gtgcgaccgg
     4381 tgccgatcgc ccagatcggc tcataggcga tgaccatctc ggccactgca gcggcggcgc
     4441 aaccggccaa cccggcgcgg acctggccga gcaccttgtc ttcggtgcca ccggcctcgc
     4501 gttcggcgag ggtctcgccg acacacatga tcggcgtcat gccgtgctgc gcgatcgcgt
     4561 gcaccttctt gttgacgtcc tcgtcggtct cggcgaacag ctcacggcgc tcgctgtgcc
     4621 cgcagatgac gtaccgcacg ccgagcttgg ccaagaacac cggcgagatc tcaccggtga
     4681 acgcgccctt gtcctgccag tggcagttct gcgcgccaag accgaacagc aactcgtcgg
     4741 cctcgatcaa ggtctggatg ctgcggatgt cggtgaacgg cgggtggatc gtgacgtcga
     4801 ccgctgcgaa gtcgtccttg gtcactaggt acgacaactt ctgcaccgtc tggatcgcct
     4861 cgaagtggtt gttgtgcatc ttccagttgc ccgagatgat agggatccgg ccggacgatg
     4921 cgctcgcggc ccgagcgcgg tgcggttcag cggggggcat tgggtgctcc tctcaacgcg
     4981 gcaagaccgg gcagatcgcc caactccaac agctccagcg acgcgccgcc gcccgtcgac
     5041 acgtgatcga cctggtcgag gccgaactgg gccagtgcag cagccgagtc gccaccgccg
     5101 accacggtga acgccttggt gtcggccatc gcctgcgcca gcgtgcgcgt cccggccgcg
     5161 aagcgctcgt cttcgaacat gcccatcgga ccgttccaga acacggtgcg ggcatccatc
     5221 acgatgtcgc taaacgcggc ggccgaaccc ggcccgatgt cgaagccctt ggctccctcg
     5281 ggcaagcggg tgccgaacgt ggcgtacgca cccgaggcat cgaccccggt gatgtcttcg
     5341 ggcaggtgga tctgcttgcc cccggcgagc aaccggcggc acgtgtcgac ctgatcgggt
     5401 tcgaacagcg agtcaccgat cggcttgccc tggggcgcga agaaggtgaa gcacatcgcg
     5461 ccgccgatga tcagcgagtc gaccacgttc aacagcgcct cgaccacgcc gagcttgtcg
     5521 gagatcttgg ccccgcccag caccgcgacg aacgggcgct tggggtggtc gcgcagaccg
     5581 agcagcacct cgacctcgcg ctgcagcaac agccccatcg ccgacggcaa cgtctgcggc
     5641 gggccgacga tggacgcatg ggcgcggtgg gcggcaccga acgcgtcgtc gacgtaggcg
     5701 tcgatgccgt cgatcaacgt agcgacgaac gcgggatcgt tgccctcctc accgggatgg
     5761 aaacggaggt tctccatcag ttccacgccc ggcgccaact cggccaggcg ttcgcgcacc
     5821 ggagccatgc tgtacttcgg ctcgggacgg cccttaggac ggccgaggtg actggcggtg
     5881 acgaccgatg cgccccgctc ggtcagccag ttgatcgtgg gcagcgcggc gcggatacgg
     5941 aagtcgtcgg tgatttcgcc gtcgtccatc ggcacgttga agtcggtgcg cacgagcacc
     6001 cgcttg
//
LOCUS       DAORNF010000109         5911 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_9987_length_5911_cov_1.890198, whole genome shotgun sequence.
ACCESSION   DAORNF010000109 DAORNF010000000
VERSION     DAORNF010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 5911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5911
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9987_length_5911_cov_1.890198"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..721)
                     /locus_tag="PLV68_05855"
     CDS             complement(<1..721)
                     /locus_tag="PLV68_05855"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HRE00803.1"
                     /translation="MRWGSSSGDEWLRDSLTRGRVTVGGRGHPVAGGMLETAENLRRM
                     YSIGRGEQDELALRSHQRAVDAQRRGAFDDEIVPVSVPGRKQAVDVVRDENPRGDTTL
                     AALGGLTPVLVASDPHATVTAGNASAQNDGAALCIVTHRGRADELGLRPLARLVAWSG
                     AGVAPEIMGIAPVAATAAALGRAGLTLTDIDLIELNEAFAAQVLACTREWGFGDGDFD
                     RLNVNGSGISLGHPVGATGTRI"
     assembly_gap    736..757
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<758..1098)
                     /locus_tag="PLV68_05860"
     CDS             complement(<758..1098)
                     /locus_tag="PLV68_05860"
                     /inference="COORDINATES: protein motif:HMM:NF012336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="steroid 3-ketoacyl-CoA thiolase"
                     /protein_id="HRE00804.1"
                     /translation="MRDIVICEPMRTPVGRYGGALRPVAAADLATTVLTALLRRTGLD
                     PSVIGDVVLGHGFPSSEAPAIGRIVALDAGLPLEVTGMQVDRRCGSGLQSVLVAAMLV
                     QTGAHEVVVAGG"
     assembly_gap    1161..1293
                     /estimated_length=133
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1294..>1635)
                     /locus_tag="PLV68_05865"
     CDS             complement(<1294..>1635)
                     /locus_tag="PLV68_05865"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HRE00805.1"
                     /translation="EDPNLLDAGKRAVTLVAGGSYMSHADSFAISRGGHLDVCVLGTY
                     EVTADGDLANWSLGGTTVPAVGGAMDLAVGARNVYVMTQHNVKDGSPKLVRRTTLPLT
                     AASVVTRIYTDL"
     assembly_gap    1636..1673
                     /estimated_length=38
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1815..2486)
                     /locus_tag="PLV68_05870"
     CDS             complement(1815..2486)
                     /locus_tag="PLV68_05870"
                     /inference="COORDINATES: protein motif:HMM:TIGR02429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit A"
                     /protein_id="HRE00806.1"
                     /translation="MAIDKIVPDVASALDGIADGSTLLVGGFGGAGTPQALLDELAGR
                     GLRDLTVIANNAGTGRTGLAALLAAGSVRKVICSYPRMPGSVVFDDLYDRGQIELELT
                     PQGTLSERIRAGGAGIGGFYTPTGAGTQLAEGREVRILGGRAHVFETPLTGDFALVRA
                     RCADRWGNLVYDKVGRNFGPTMAMAATTTIVETDEVVALGELDPEQIVTPGIFVQRVV
                     KVRAA"
     gene            complement(2493..3242)
                     /locus_tag="PLV68_05875"
     CDS             complement(2493..3242)
                     /locus_tag="PLV68_05875"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRE00807.1"
                     /translation="MTEKYLFLDVEVRDGVAVATMQHPDYSHAERNDWVTLINAVKDD
                     DDVRALVVTGWGNPKQGPVPGMFEDFDAYMYYERARLQPLAMFIDLDKPVVMAIDGNP
                     GVMSIPLTGDILIGQSDLTFSDPHVLIGTSSATQPYLWPLNSGLLQAKKYILTGKSFD
                     AAEALALGLLTEVVEPGQTLEKAMEYATHFASLRPETLQATKRVLNQWLRQFHASIFE
                     PALMMEFMSFPKNFADRFKPAATSAADDDAG"
     gene            3377..4534
                     /locus_tag="PLV68_05880"
     CDS             3377..4534
                     /locus_tag="PLV68_05880"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRE00808.1"
                     /translation="MGTDQMEIRADVREVVKGVTALVDRQEFVRRANSGEDLDDVWQT
                     MAELGLLGVGLPEEYGGSGAGMTGPVALVEAMSEAGTPPVLYLLTAFARMAILRHGTE
                     EQKRRFLTTTTTGEVRLAFAITEADAGTNSFRMQTFAREDGGHYALSGEKVFISAADQ
                     ATHVMVVARTTRLKDTEDKRVGLSLFVVPIDSPGLQLTRMKIDLHSPEHQFVLSLDEV
                     GVPAENLIGQRDEGLRALFDALNPERLLVSAWAIGLGTHALQRGVDYARERAPFGTPI
                     GSYQGVQHPLARARAHLDAARLMMYTACDAYDSGDDAGANANMAKLLASEAANAAADA
                     VIQAFGGSAFDQDSDIVTLWPMIRLLRIAPVNNEMILNYISEKILRLPKSY"
     gene            4556..>4843
                     /locus_tag="PLV68_05885"
     CDS             4556..>4843
                     /locus_tag="PLV68_05885"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRE00809.1"
                     /translation="MDAWIDDDGDRRIRAAVAGQTVPRLFAATVARRPDEVAHRWRTA
                     DGWATWTWREYADHACRTATLIEQLGVPRGGKVALMLRNCHEFHPIDVGAYL"
     assembly_gap    4844..4916
                     /estimated_length=73
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4938..>5263
                     /locus_tag="PLV68_05890"
     CDS             4938..>5263
                     /locus_tag="PLV68_05890"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRE00810.1"
                     /translation="MFVEPDFAPQVRAAIAQIGSAVRVVVVGGTGDDTWKARIAASSP
                     VDLDAAAARVTPEDLATLIYTSGTTGPPKAVMLSHGNVCWLLESLVQVMGFDLGVHQG
                     LDFVGI"
     assembly_gap    5264..5273
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5274..>5524
                     /locus_tag="PLV68_05895"
     CDS             <5274..>5524
                     /locus_tag="PLV68_05895"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00811.1"
                     /translation="AHVAERVFTHYLPLREGALVTPCPNPAEVGAYLPEVRPEAFFGP
                     PRVFEKMRGALIGALSATEERTEELFAAVRAKVGLDACRV"
     assembly_gap    5525..5654
                     /estimated_length=130
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5655..>5911
                     /locus_tag="PLV68_05900"
     CDS             <5655..>5911
                     /locus_tag="PLV68_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRE00812.1"
                     /translation="VRQPRVGTVGRPIPGCSVRLLDDGEILCRGGHVFGGYFKDPDRT
                     AEVLDDDGWLHTGDIGVLGDDGYISIVDRKKELIITAGGKN"
BASE COUNT          952 a         1866 c         1800 g          887 t
ORIGIN      
        1 ggatgcgcgt acccgtcgcg ccaacgggat gtccgaggga gatgcccgat ccgttgacgt
       61 tgagccggtc gaagtcgccg tcgccgaaac cccactcgcg cgtgcaagcc aacacctggg
      121 ccgcgaaggc ttcgttgagc tcgatcaggt cgatgtcggt caacgtcaat ccggcccgcc
      181 cgagcgccgc cgcggtcgcc gccaccggcg cgatgcccat gatctcgggg gcgacgcctg
      241 ctcccgacca cgcgacgagg cgtgccagtg gccgtagccc gagctcgtcg gcacgcccac
      301 gatgggtcac gatgcacagc gctgcaccat cgttctgagc gctggcattg ccagccgtca
      361 cggtcgcgtg cggatcgctc gccaccagca cgggcgtgag tccgccgagc gcagccagcg
      421 tggtgtcacc acgcgggttc tcgtcgcgca cgacgtccac ggcctgcttc cgtccgggga
      481 cggacaccgg cacgatctcg tcgtcgaaag cgcctctgcg ttgcgcatcg acggcccgct
      541 ggtgcgagcg cagagcgagc tcgtcctgtt cgccgcggcc gatcgaatac atgcggcgca
      601 ggttctcggc cgtctccaac atcccgccgg cgacggggtg accgcggcct ccgacggtca
      661 cccgcccgcg ggtcagactg tcgcgaagcc actcgtcgcc cgacgacgag ccccatcgca
      721 tggccgtcgt gtagtnnnnn nnnnnnnnnn nnnnnnncca cccgcaacga ccacctcgtg
      781 ggctccggtc tggacgagca tcgcggcgac gaggaccgat tgcaggccag acccgcagcg
      841 ccggtcgacc tgcatgccgg tgacctccag cggcaaaccg gcgtcgaggg cgacgatgcg
      901 cccaatcgcc ggtgcctcgc tactcgggaa gccgtggccg aggacgacat caccgatgac
      961 cgacgggtcg agcccggttc gccggagcag ggctgtgagg accgtcgtgg ccaggtcggc
     1021 ggccgcgacc ggccgcagag ctccgccgta gcgaccgacg ggggttcgca tcggctcgca
     1081 gatgacgatg tcgcgcatga tccagtgacc tccactcaac aggcaatgcc gaacaggtag
     1141 cagaatgggc gtctcagccc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngagatcg gtgtagatcc gcgtcaccac
     1321 cgatgccgcc gtgagcggga gcgtcgtcct gcggacgagc ttcggactgc cgtccttcac
     1381 gttgtgctgg gtcatcacgt acacgttgcg agcaccgacc gccagatcca tcgctccgcc
     1441 gaccgcgggc acggtcgttc caccgagcga ccagttggcc aggtcgccat cggcagtcac
     1501 ctcgtaggta ccgagcacgc acacgtcgag atgcccgccg cggctgatcg cgaacgagtc
     1561 ggcgtggctc atgtaggaac ccccggcaac gagggtcacg gcgcgcttac cggcatcgag
     1621 caggttgggg tcctcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntctcgca
     1681 gtggatgacg acctcacgac cagcggggat gtgggcagcg acctggcttg gcagtccgat
     1741 gccgaggttc acgtaccaac cgtcgcgcag atcgaggccg acctcgcggg ccatctgctc
     1801 ctgggtcagc gcactcacgc cgcacgcacc ttgacgacgc gctggacgaa gatcccgggg
     1861 gtcacgatct gttcgggatc gagctcaccc aaggcaacca cctcgtcggt ctccacgatc
     1921 gtcgtggtcg ccgccattgc catggtcggc ccgaagttgc gcccgacctt gtcgtacacg
     1981 aggttgcccc accggtcggc gcagcgagcc cgcaccagcg caaagtcgcc cgtcagcggt
     2041 gtctcgaaga cgtgcgcacg gccaccgagg atgcgcacct cccgtccctc ggcaagctgg
     2101 gtccctgcac cggtcggggt gtagaagcca ccgatgcccg caccaccggc gcggatgcgt
     2161 tcggacagcg tgccctgcgg ggtcagctcc agctcgatct ggccgcggtc gtagaggtcg
     2221 tcgaagacca ccgaacccgg catgcgcggg tacgaacaga tcaccttgcg aaccgatccg
     2281 gccgcgagca gggcggccaa gccggttcgt ccggttcccg cgttgttggc gatgacggtc
     2341 agatcgcgca agccgcggcc agcgagctcg tcgagcagcg cctgaggggt gccagcacca
     2401 ccaaacccgc cgaccagcaa ggtggacccg tcggcgatgc cgtcgagtgc actggccacg
     2461 tcaggcacga tcttgtcgat tgccatcgcg gttcagccag cgtcgtcgtc cgccgcagag
     2521 gtcgccgccg gcttgaagcg atcggcgaag ttcttgggga acgacatgaa ctccatcatc
     2581 agcgccggct cgaagatcga cgcgtggaac tgtcgcagcc actgattcaa cacacgcttg
     2641 gtcgcctgca acgtctcggg acgcagcgag gcgaagtgtg ttgcgtactc catcgccttc
     2701 tccagcgttt ggcccggttc gacgacctcg gtcagcaagc cgagcgcgag cgcctcggca
     2761 gcgtcgaagg acttgccggt caggatgtac ttcttcgcct ggagcagtcc ggagttcagc
     2821 ggccacaggt acggctgcgt cgccgaggag gtaccgatca ggacgtgcgg gtcggagaag
     2881 gtgaggtccg actggccgat caggatgtca ccggtcaacg ggattgacat cacaccggga
     2941 ttgccatcga tggccatcac caccggcttg tcgaggtcga tgaacatcgc cagcggctgc
     3001 agacgggcac gctcgtagta catgtacgcg tcgaagtctt cgaacatacc cggcacgggg
     3061 ccttgcttgg gattgcccca gccggtcacg acgagagcac ggacatcgtc gtcgtccttg
     3121 acggcgttga tcaacgtcac ccaatcgttc cgttcggcgt ggctgtaatc gggatgctgc
     3181 atcgtggcga ccgcgacacc gtcacgaact tcgacgtcga ggaagagata tttttccgtc
     3241 atgtgagtcc cccaactgtg cggattcgtt tgacgtttca atcaaactca tgccaatcta
     3301 caaccgaacg accgttagaa tcaaagacgg gcttggcgca tcgatcgccc cgtcctgagc
     3361 atctgtggag cacaacgtgg ggactgacca gatggagatt cgcgccgacg tgcgcgaagt
     3421 ggtcaaaggt gtcacggccc ttgtcgacag gcaggagttc gtacgccggg ccaactccgg
     3481 cgaggacctc gacgacgtgt ggcagacgat ggccgagctc ggcttgctcg gtgtcggact
     3541 tcccgaggag tacggcggca gcggcgccgg gatgaccggt ccagtcgcgc tcgtcgaggc
     3601 gatgtcggag gctggcacac ctcctgtgct ctacctgctg actgccttcg ccaggatggc
     3661 gatcctccgc cacggcaccg aggagcagaa gcggcgcttc ctgaccacga ccacgaccgg
     3721 cgaggttcgg ctcgccttcg cgatcaccga agcagacgcc ggcacgaaca gcttccggat
     3781 gcaaacgttt gcccgcgagg atggcgggca ctacgcgctc agcggcgaaa aggtcttcat
     3841 ctccgcggcc gaccaagcca cacacgtgat ggtcgtcgcc cgcaccactc gcctgaagga
     3901 caccgaggac aagcgcgtcg gcttgtcgct gttcgtcgtg ccgatcgatt cgcccggtct
     3961 gcagctgacc aggatgaaga tcgatctgca ttcaccggag caccagttcg ttctcagcct
     4021 cgacgaggtc ggggtgcccg cggagaacct catcggccag cgcgacgagg gcttgcgggc
     4081 cttgttcgat gcactcaatc cagaacgcct gctggtatct gcttgggcga tcggccttgg
     4141 tacgcatgca cttcagcgcg gtgtcgatta tgcgcgcgag cgggccccgt tcggtacgcc
     4201 gatcggctcc taccaaggcg tgcagcaccc cctcgcccgg gcgcgagcgc acctcgatgc
     4261 cgcccggctg atgatgtaca cggcgtgcga cgcctacgac agcggcgacg acgcaggcgc
     4321 caacgccaac atggccaagc tcctggcctc ggaggcggcc aacgcggcag ccgatgcggt
     4381 catccaagcc ttcggcggat cggccttcga tcaggacagc gacatcgtca ccctgtggcc
     4441 gatgatccga ctgctgcgca tcgcaccggt caacaacgag atgatcctca actacatctc
     4501 cgagaagatc ctccgcctgc cgaagtcgta ctgaccgaga gggaaagggg accccatgga
     4561 tgcctggatc gacgacgatg gtgaccggcg gatccgcgcc gcggtcgcgg gccagacggt
     4621 gccacgtctg tttgcagcga ccgtcgcccg ccgccccgac gaggtcgctc accgatggcg
     4681 aaccgccgac ggctgggcga cgtggacgtg gcgcgaatac gccgaccatg cgtgccgcac
     4741 ggcgacgctg atcgagcagt tgggcgtacc gcgcggcggc aaggtggcgt tgatgttgcg
     4801 caactgtcac gagttccatc cgatcgacgt cggcgcctat ctcnnnnnnn nnnnnnnnnn
     4861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngacc
     4921 actccgaggc atcactcgtg ttcgtcgagc ccgacttcgc cccgcaggtt cgcgccgcca
     4981 tcgcgcagat cggctcggcg gtgcgtgtcg tggtcgtggg cggcaccggt gacgacacgt
     5041 ggaaagccag gatcgcggcc agctcgcccg tcgacctcga cgccgctgcg gcccgcgtca
     5101 cacccgaaga cctcgccaca ttgatctaca cctcaggaac gaccggtcct ccgaaggccg
     5161 tgatgctctc gcacggcaac gtctgctggc tcttggaatc actggtccag gtgatgggtt
     5221 tcgatctcgg cgtccatcag ggactcgatt tcgtgggcat tgannnnnnn nnngcccacg
     5281 tcgccgagcg cgtcttcacg cactacctcc cgctgcgcga aggcgcgctg gtcacgccct
     5341 gccccaaccc ggccgaggtg ggcgcgtacc tgcccgaagt ccggccagag gccttcttcg
     5401 ggccaccgcg cgtcttcgag aagatgcgcg gcgctctgat cggcgcactg tcggccaccg
     5461 aggagcggac agaagagctg ttcgcggcgg tgcgcgccaa ggtcggcctc gatgcctgcc
     5521 gcgtnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5641 nnnnnnnnnn nnnngtccgc caaccgcggg tcggcacggt cggtcgcccg attcccgggt
     5701 gctcggtgcg cctgctcgac gacggcgaga tcctgtgtcg aggcgggcac gtcttcgggg
     5761 gctacttcaa ggatccggac aggacggccg aggtgctcga cgacgacggc tggctacaca
     5821 ccggtgacat cggcgtgctc ggcgacgacg gctacatcag catcgtcgac cggaagaagg
     5881 aactgatcat cacggctggc ggcaagaaca t
//
LOCUS       DAORNF010000110         5900 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_10006_length_5900_cov_1.595381, whole genome shotgun sequence.
ACCESSION   DAORNF010000110 DAORNF010000000
VERSION     DAORNF010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 5900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5900
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10006_length_5900_cov_1.595381"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="PLV68_05905"
     CDS             complement(<1..259)
                     /locus_tag="PLV68_05905"
                     /inference="COORDINATES: protein motif:HMM:NF013869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MscL family protein"
                     /protein_id="HRE00813.1"
                     /translation="MADNNKGLIKEFRDFIASGNMIELAVAVILGAAVGAMIKAFTDG
                     IMMQIVAAIFGQPDFSAIKITLRKNVGTDADGNPTDSVLQIG"
     gene            383..1772
                     /locus_tag="PLV68_05910"
                     /pseudo
     CDS             383..1772
                     /locus_tag="PLV68_05910"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
     assembly_gap    1874..2077
                     /estimated_length=204
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2078..2624)
                     /locus_tag="PLV68_05915"
     CDS             complement(<2078..2624)
                     /locus_tag="PLV68_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRE00814.1"
                     /translation="MSDTTSPSPAAAIDLTDLFRLDGQVAIVTGASSGLGDRFARVLH
                     AAGATVVLAARRLDRLEALAGQLPGSLAVATDVSIPEDRERLVATTLERLGRVDVLVN
                     NAGVGGGVAIEDETLDQFRQAMEINVTAIWHLIKLVAGPMREQGYGSIINIASVLGHV
                     ASSPIKQAHYTASKGAVVNLTR"
     assembly_gap    2647..2666
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2781..3077
                     /locus_tag="PLV68_05920"
     CDS             2781..3077
                     /locus_tag="PLV68_05920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00815.1"
                     /translation="MGHSDVCIPMSTHRTPLSTINRTPARFGLASALMLTLGVAAAAW
                     FLADVAQLSERTIVLGVMAVSFVASWVLTGVRRKPMSHHRVTVVPHHARPVSSR"
     gene            3160..>3628
                     /locus_tag="PLV68_05925"
     CDS             3160..>3628
                     /locus_tag="PLV68_05925"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HRE00816.1"
                     /translation="MKVRIGFGFGTTTNLNDDSFGPVIDAMERLRFDSLWLSERIGAN
                     APDPLVAMAYAAGRTTRLKFGMSVMVLPGRNPVVLAKELATLDRLSGGRLLPAFGLGV
                     ASGNEQQAFGVARTERAKIFDEALAVVRACWTNERVDFAGTYFHYDGLKVLPKP"
     assembly_gap    3629..3770
                     /estimated_length=142
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3771..4055
                     /locus_tag="PLV68_05930"
     CDS             <3771..4055
                     /locus_tag="PLV68_05930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00817.1"
                     /translation="DREVPADHFGALIPYTHQPLPDAVLATLAARRTDLDDPRALVPC
                     GWDALLATIDRFVDVGTTKFVVLPIVEPNGAEAWIAELEAAAATLLPRQT"
     gene            4178..>4889
                     /locus_tag="PLV68_05935"
     CDS             4178..>4889
                     /locus_tag="PLV68_05935"
                     /inference="COORDINATES: protein motif:HMM:NF037180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00818.1"
                     /translation="MVSPGAVPAGRHRLVVRPHDIPERGVTITFQVGTIDADLDAEQR
                     RQIETVLVDPLLETGRWDEPVGAGQLDIGLIPGVTDTAAASFRYAATLLGIPVRDVAV
                     GRLIDIDVNHDTADTAGRTWANAVIERWAVGHAVEPGYAAPAAAVTATESVPLPDDDE
                     ALTTLGRERGLALDLAELHVIRRHFADLGRDPTDAELETLAQTWSEHCAHKSFRAEII
                     ATEPDGSPLHIDPLLTQLR"
     assembly_gap    4890..5018
                     /estimated_length=129
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5019..>5514
                     /locus_tag="PLV68_05940"
     CDS             <5019..>5514
                     /locus_tag="PLV68_05940"
                     /inference="COORDINATES: protein motif:HMM:NF012795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HRE00819.1"
                     /translation="IEPFGGANTGVGGVIRDIVGAHHHAIACTDILCFGPADTPIESL
                     PDGVLHPRRIIDGVIAGVADYGNKIGLPNVAGAVLYDPAYVSNPLVFAGCIGVAPAGR
                     PALTGPHPGDHIVVLGGRTGRDGIRGATFSSMEMDATTGDVAGASVQIGDPIVEKLVS
                     DMLAE"
     assembly_gap    5515..5588
                     /estimated_length=74
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5589..>5900
                     /locus_tag="PLV68_05945"
     CDS             <5589..>5900
                     /locus_tag="PLV68_05945"
                     /inference="COORDINATES: protein motif:HMM:NF014788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HRE00820.1"
                     /translation="IGADVELTTVPLKYAGLAPWEIWLSEAQERMVIAVDAGSLDAVR
                     DLAARHGVEMCDVGTFTGDGQLVVRYDGTPIVDLDTAFLHDGRPRRRLDAVLPTPLRT
                     RV"
BASE COUNT          841 a         1853 c         1817 g          820 t
ORIGIN      
        1 cgccgatctg caacacgctg tcggtcgggt tgccgtccgc gtccgtgccg acgttcttgc
       61 gcaacgtgat cttgatcgcg gagaagtcag gctgaccgaa gatcgcggcc acgatctgca
      121 tcatgatccc gtcggtgaag gccttgatca tcgcgcccac cgcggcgccg aggatgacgg
      181 cgacggccag ttcgatcatg ttgccggagg caatgaagtc tcggaactcc ttgatgagtc
      241 ccttgttgtt atccgccatt gatggggttc ccttctcggt gtgtgggggg tcgaaggctc
      301 attgaaacac gcgccgccac cgggacgagg gcttttctgc tacacacagg ccatgagctc
      361 tcttgccgac caaacccgct ggatggatgc gaccgaccag gccggcctgg tcgcagccgg
      421 gcaggtcacc ccactcgaac tgttggatgc tgcgatcgag cggatcgaac ggatcgaccc
      481 ggcgctcaac gcgctgacct accgctggtt cgaccacgcc cgcgagaccg cggcaggtga
      541 gttgcccgac gggccgttcc gcggtgtgcc gttcttgctc aaggacctct acgccaccta
      601 cgccggacag ccgatctcca acggcagcgt ggcgctgaag aacgccgggc agttggccgc
      661 ggccgatacc acgctggtgg cacgccatcg ggccgccggg ttcgtcatcg ccgggcgcac
      721 caacagcccc gagctcggca gcgtgccggt gaccgagccg gtggcgtggg ggccgacccg
      781 caacccgtgg aacaccgatc acacaccggg cggctccagc ggcggcgcgg ccgctgccgt
      841 tgcgtcgggc atggtgccga tcgctcacgc cagcgacggc ggaggttcga tccgcatccc
      901 cgcgtcgtgc tgcgcccagc cagggccgga tcactctggg gccggtacgc gacgaaagcg
      961 ggttgggcgt cgagcactgc gtcagccgta ccgtgcgcga caccgcggcg ctgctcgacg
     1021 ccacgtgcgg gccgggcatc ggtgacaccg tcatcgctcc ggcgcccgcg cgtccgtatc
     1081 tggccgaagt cggcgccgat cctggccggc tgcgcatcgg cgtgctcgac caccatccct
     1141 tcggcggcac gatcgacccc gaatgcacgc gcgccgcggc caagacggcg tcgatgctca
     1201 gcgcgctcgg ccatgacgta cacgaggcgt ggccagcccc gctcggcgac gccacgttcg
     1261 cggccaagtt catggcgctg tgggccacca acatgggcgt cgcccgggcg cggttcgaag
     1321 atcaactcgg tcggccactg gccgacgacg agatggagct gatgaaccgc gtccaggccg
     1381 actacgccgc caacttctcg gccagtgact acgccctgtc actcagtgcc gtggcccagt
     1441 ttcgccgggc ggtacaggcg tggtggcacg agggctggga cctgctggtc accccgacgc
     1501 tcggccagga accggtgccg atcggcacca tcgccaacaa ccccgccaac ccgatggagc
     1561 cgatggccaa ggcagggctg ttcgtgccgt tcaccccggc gttcaacagc accggccaac
     1621 ccgcgatcag cctgcccctg cactggtcgc ccagcggctt gccgatcggt gtgcagttcg
     1681 tggccgccta cggccgcgaa gacgtgctga tccgcatcgc cagccaactc gaagcggccc
     1741 acccgtgggc cgaccgccac cccgacatct gatcagcgcc gcgagtcagc ggttgggacc
     1801 agcagttggg atcaggggtt gggatcagcg ggcggtccag ccgccgtcga ccagcagcga
     1861 ctggccggtg atannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncgc gggtcaggtt gaccacggcg
     2101 cccttcgatg ccgtgtagtg ggcctgcttg atgggcgagc tggcgacgtg gcccagcacc
     2161 gacgcgatgt tgatgatcga gccgtagccc tgctcgcgca tcggccccgc caccaacttg
     2221 atgagatgcc agatcgccgt gacgttgatc tccatcgctt gacggaactg atcgagcgtc
     2281 tcgtcttcga tcgcgacccc gccgccgacg ccggcgttgt tgaccaacac gtcgacgcgc
     2341 ccgaggcgct caagcgtggt ggcaaccaac cgctcacggt cttcggggat ggagacgtcg
     2401 gtcgcgaccg ccaacgaccc agggagttgg ccggcgagcg cttccagccg gtcgaggcgt
     2461 cgtgcagcga gcaccaccgt cgcgccggcg gcgtgcagga cgcgggcgaa tcgatcaccg
     2521 aggccggacg acgcgccggt gacgatggcc acctggccgt cgaggcggaa cagatcggtg
     2581 aggtcgatgg cagcggccgg gctgggagag gtggtgtcgc tcatggccgc cgagattagg
     2641 acgcacnnnn nnnnnnnnnn nnnnnngtgc gacgaatggg tgaccgccag atggaccggt
     2701 cgtcacgaac gtggcgacgg tgttacgacc tggtcacgaa cgtgacagtt tggaaaaggt
     2761 tggtctgagt gggatcaaaa gtggggcact ctgacgtctg tatccctatg agcacgcacc
     2821 gcactccgct ctcgacgatc aaccgcacac cggcccggtt cgggctggca tccgcgttga
     2881 tgctgacgct cggtgtggcc gcggcggcgt ggttcctggc cgacgtcgcg cagttgtccg
     2941 agcgcacgat cgtgctgggc gtgatggccg tgtcgttcgt tgccagttgg gtcctgaccg
     3001 gggtacggcg caagcccatg tcgcaccacc gggtgacggt ggttccccac cacgcccgcc
     3061 ccgtcagctc acgctgaacg cgtccggcat gacacgatgc cgggcatgac gtcactgccg
     3121 acaccgtcat tgccgacacc aaagatgccg acaccaaaga tgaaggtgcg gatcgggttc
     3181 gggttcggca ccacgaccaa cctcaatgac gacagcttcg gtccggtgat cgacgcgatg
     3241 gagcggctgc gcttcgacag cctgtggctg agcgagcgga tcggcgccaa cgcacccgat
     3301 ccattggtgg cgatggccta cgccgcgggt cggaccaccc ggctcaagtt cgggatgagc
     3361 gtgatggtgc tgcccgggcg caatccggtg gtgctggcca aggagttggc caccctcgac
     3421 cgtttgagcg gcggacggct gctgccggcg ttcggactgg gcgtggcaag cggcaacgag
     3481 cagcaggcct tcggtgtggc ccgtaccgaa cgggccaaga tcttcgacga ggcgctggcc
     3541 gtggtgcgcg cctgttggac gaacgagcgg gtcgacttcg ccggcaccta cttccactac
     3601 gacggcctga aggtgctccc caagccgcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gatcgcgaag
     3781 tgccggccga ccacttcggg gcgctgatcc cctacaccca tcaaccgctg cccgacgcgg
     3841 tgctcgccac actggccgcg cgccggaccg acctggacga tccgcgggcg ctggtgccgt
     3901 gcgggtggga cgcgctgctc gccacgatcg accgcttcgt cgacgtgggc accacgaagt
     3961 tcgtggtgct gccgatcgtc gagccgaacg gcgccgaggc gtggatcgcc gagctggaag
     4021 cggccgccgc gaccctgctg ccgcgccaga cctgactggg ccaacctgaa ccggttcggc
     4081 cgccggcgat ggctcggcgg ccgctgcagt accggtcgca ttgacttttc gttcatcccg
     4141 caccgccccg tgggaggggg tgcctagggt catcgtcatg gtctcccctg gtgcggtgcc
     4201 ggccggcagg caccgcctcg tcgtccgccc ccacgacatc cccgaacgtg gtgtcacgat
     4261 cacgtttcag gtcggcacga tcgacgccga cctcgacgcc gagcagcggc gccagatcga
     4321 gaccgtactc gtcgatcccc tgctcgaaac aggccgctgg gacgagccgg tcggagccgg
     4381 tcaactcgac atcggactga tccccggcgt caccgacacg gccgcggcgt cgttccgcta
     4441 cgcggcaacg ctgctgggca ttccggtgcg cgacgtggcc gtcggccgcc tgatcgacat
     4501 cgacgtgaac catgacaccg ctgacacagc cgggcggacg tgggccaacg cggtcatcga
     4561 acggtgggcc gtcggtcacg cggtcgaacc cggctacgcc gcaccagccg cggccgtcac
     4621 cgcgaccgag tcggtcccac tgcccgacga cgacgaagcg ctgacgaccc tcggtcgcga
     4681 gcgcggcctg gcgctcgacc tggccgaact gcacgtgatc cgccgccatt tcgccgatct
     4741 cggccgtgac ccgaccgatg ccgagttgga gacgctggcc cagacctgga gcgagcactg
     4801 cgcgcacaag agcttccggg ctgagatcat cgccaccgag ccggacggca gcccgctgca
     4861 catcgaccca ctgctcaccc agttgcgtcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnat cgagccgttc ggcggcgcca
     5041 acaccggcgt cggtggggtc atccgcgaca tcgtcggtgc gcatcaccat gcgatcgctt
     5101 gcaccgacat cctgtgcttc ggcccggccg atacgcccat cgagtcgctg cctgacggcg
     5161 tgttgcaccc gcggcggatc atcgacgggg tgatcgcggg cgtcgccgat tacggcaaca
     5221 agatcggcct gccgaacgtg gccggagccg tgctgtacga cccggcctac gtcagcaacc
     5281 cgctggtgtt cgcggggtgc atcggtgtcg ccccggcagg gcggcccgcg ctgaccggcc
     5341 cgcaccccgg cgaccacatc gtcgtgctcg gtggccgcac cgggcgcgac ggcatccgcg
     5401 gcgcgacgtt ctcgtcgatg gagatggacg ccaccaccgg cgacgtcgcc ggggccagcg
     5461 tgcagatcgg tgacccgatc gtggagaagc tcgtcagcga catgctcgcc gaagnnnnnn
     5521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5581 nnnnnnnnat cggcgccgac gtcgaactga ccacggtgcc gctcaagtac gccgggctcg
     5641 cgccgtggga gatctggctc agcgaagcgc aggaacggat ggtgatcgcg gtcgatgccg
     5701 gttcgctcga cgccgtccgc gacctggccg cgcgccacgg tgtggagatg tgcgacgtcg
     5761 gcaccttcac cggcgacggg caactggtgg tgcgctacga cggcacgccg atcgtcgacc
     5821 tggataccgc gttcttgcac gacggccgcc cgcggcgacg cctcgacgcg gtgctgccga
     5881 cgcccttgcg cacccgcgtg
//
LOCUS       DAORNF010000111         5893 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_10019_length_5893_cov_1.751456, whole genome shotgun sequence.
ACCESSION   DAORNF010000111 DAORNF010000000
VERSION     DAORNF010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 5893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5893
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10019_length_5893_cov_1.751456"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..644
                     /locus_tag="PLV68_05950"
     CDS             <1..644
                     /locus_tag="PLV68_05950"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRE00821.1"
                     /translation="VDHIDLYYMHRADHRVPIEETIGVLSELVAAGKVGHIGLSEVTA
                     DTVRRAAAVHPIAALQSEWSLFSRGLKSDVVPTCREFGVAIVPFSPLGRGILTGTLTT
                     ALGAGDMRANHPRFQGEAFNANLRSVDTVREVAAELGATPGQVALAWLLAKGGDVVPI
                     PGTKRIERLDENLGADAITLSADQVSRLDQVTAVGHRATDPTWVDRDTPPLPG"
     gene            766..1740
                     /locus_tag="PLV68_05955"
     CDS             766..1740
                     /locus_tag="PLV68_05955"
                     /inference="COORDINATES: protein motif:HMM:NF013722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HRE00822.1"
                     /translation="MFDITRPGPFYEDFSVGATLPPLPSVTLTEADNTLYRAMTGDQH
                     LLSSDRRLAAAVMGHDRGIANPGLVMQYSIGQTTMATRQAIANLYYRSVRILRPVELG
                     ETISTVTTVLGLADSTPKGDQYRGKVWLGITTSAAAGAVAAYERCALVRGNTSTLPGH
                     ASAIPGPSDATPLAEFVASLPEWDLSPLPRTEWATGTALSDPLRDHIDLAAPLARWSF
                     NQAAVHRDVTATANGRRLVYGGHVQGLAQASLTRMAPGVALVLGWDGCDHIGPAFEGD
                     LLEFRHEVIEALPAGGGRLLRLSVTGTVVSDDGARTDVLRWTPIVWSA"
     gene            complement(1786..2184)
                     /gene="msrB"
                     /locus_tag="PLV68_05960"
     CDS             complement(1786..2184)
                     /gene="msrB"
                     /locus_tag="PLV68_05960"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HRE00823.1"
                     /translation="MAEIERTDDEWRTRLDPLSYAVLREAATERAFTGELLKEKRDGL
                     FTCKGCGNELFKSGTKFESGGWPSFYESVRPEAVTLLADHTLGMERTEVRCARCDSHL
                     GHVFPDGYGTPTGLRYCMNSVALEFNPADT"
     gene            complement(2361..2780)
                     /locus_tag="PLV68_05965"
     CDS             complement(2361..2780)
                     /locus_tag="PLV68_05965"
                     /inference="COORDINATES: protein motif:HMM:NF024304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HRE00824.1"
                     /translation="MAELAAWECYDLLSSEGLGRMCVIDRGYPIAYPVNYRLVQTVGT
                     PRIIFRTSPNAAMTRCEGAASFEVDHVSPARGQAWSVIARGHLRRLFGVQHLPDTMPL
                     VSIQRHQWMELDITSVTGRRFVVAGQDEFSTEWTLAT"
     gene            complement(3047..3934)
                     /locus_tag="PLV68_05970"
     CDS             complement(3047..3934)
                     /locus_tag="PLV68_05970"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRE00825.1"
                     /translation="MHEGSFTDTHGIDVFTRTWPVADPKGLVLIAHGASEHSGRYDRF
                     ARALNAAGYAAAALDHRGHGNSAAVTGVGRTGPGGGSAVIEDLHQLRTLTSTEVGPET
                     PVFLFGHSLGSLIGLAYLVQHSAGLAGGILCGFPANLADPGGLAEMLAAVDAAGLCDE
                     SAAPVLAANNAAFEPARTPYDWLSRDEAEVDAYAADPFCGDDYPITFGYLIDIFGVVG
                     PAAESLGSIACPVLVIAGDKDPAAGMGEFPATLAAALGAAGVSADLTLYPDARHEILN
                     ETNRDEVTADTIAWLARHT"
     gene            complement(3973..4404)
                     /gene="trxA"
                     /locus_tag="PLV68_05975"
     CDS             complement(3973..4404)
                     /gene="trxA"
                     /locus_tag="PLV68_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HRE00826.1"
                     /translation="MSEIVTCPSCGTKNRVPVVAAGTPRCASCKAALAWLVNAGDADF
                     DAAVDTTALVLVDLWAPWCGPCRMVAPGVEQAAAELAGRLKAVKVNVDEAPGISARFG
                     AQSIPTLLLMRGGKVVERQVGALPAHRLLAWIKQALVLHPA"
     gene            complement(4433..5218)
                     /locus_tag="PLV68_05980"
     CDS             complement(4433..5218)
                     /locus_tag="PLV68_05980"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRE00827.1"
                     /translation="MHDEQHPAGPAPDHGHDDGHAHDHTHSHTPDHGQRHAHSHDHHS
                     HDQHGDDHHSHDHGDEYGASHFDERAATWDDNPDHVVRAQVVADAIAAAIPLDGSTRL
                     LDYGAGSGLFTQAIGGRVGSAVLADSSSGMRAVIQGKIDAGLIRNATVSALDLSHDAP
                     GQARFDLIATILTLHHVADLTPVLSGFAEMLTPGGHLCIVDLDKEDGSFHPGGFGGHH
                     GFDHDDLRRQLAEAGFDQVEFSRCHQIVRDGAEYPMFLAICAR"
     gene            complement(5223..>5507)
                     /locus_tag="PLV68_05985"
     CDS             complement(5223..>5507)
                     /locus_tag="PLV68_05985"
                     /inference="COORDINATES: protein motif:HMM:NF015581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HRE00828.1"
                     /translation="DVATVLRAKLGIERPPLKILGACNPNLAHRALESDADAALMLPC
                     NVVLEPVEGGTRVRAADPRQMMPGAEMAELATEAAGRLTAALSALPGAAH"
     assembly_gap    5508..5683
                     /estimated_length=176
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5707..>5893)
                     /locus_tag="PLV68_05990"
     CDS             complement(5707..>5893)
                     /locus_tag="PLV68_05990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00829.1"
                     /translation="FAGCGRHVEMVLGDVALDQRCDCAERLRSGKAPASAASVTSQDG
                     VSCGIAGRMRSWLKRRR"
BASE COUNT          852 a         2006 c         1981 g          878 t
ORIGIN      
        1 gagtcgacca catcgacctc tactacatgc accgcgccga ccatcgtgtg ccgatcgaag
       61 agaccatcgg cgtgctctcc gaactggtcg cggcgggcaa ggtcggccac atcggactgt
      121 ccgaggtcac ggccgacacg gtgcggcgcg cggccgcggt gcatccgatc gccgcgctgc
      181 agagcgagtg gtcgctgttc agccgcgggt tgaaaagcga cgtcgtgccg acgtgtcgcg
      241 agttcggcgt cgccatcgtg ccgttcagcc cgctgggccg aggcatcctc accggcacgc
      301 tcaccaccgc gctcggtgcc ggcgacatgc gcgccaacca ccctcgcttc cagggcgagg
      361 cgttcaacgc caacctgcgc agcgtcgaca cggttcgcga ggtcgcggcc gaactcggcg
      421 cgacgccggg ccaggtcgcg ttggcgtggt tgctggccaa gggtggcgac gtggtgccga
      481 tccccggtac caagcgcatc gagcggctcg acgagaacct cggtgccgac gcgatcacgc
      541 tgtcggccga ccaggtgtcc cgactcgatc aggtcaccgc ggtcggccac cgcgcgaccg
      601 acccgacgtg ggtcgaccgc gacacgccgc cgctgcccgg ctgaccggct gaccggctga
      661 ccggctgact gtgaccggct gaggctgcgg gttccaccga ccgggcccga tcacagcgtc
      721 ggccacattg gcagtcgccg gcccacatcg gtgagactgc gtgtcatgtt cgacatcacg
      781 cgtcccggac cgttctacga agacttcagc gtcggcgcca cgctgccccc gctgccgagc
      841 gtgacgctga ccgaggccga caacacgctg taccgggcga tgaccggcga ccagcacctg
      901 ctgtcgtccg accgtcgcct cgccgcggcc gtcatgggcc acgaccgggg catcgccaac
      961 cccggcttgg tgatgcagta ctcgatcggg cagacgacga tggcgacccg ccaggcgatt
     1021 gccaacctct actaccgctc ggtgcgcatc ctgcgcccgg tcgagctcgg cgagacgatc
     1081 agcacggtca cgaccgtgct cggcctggcc gactcgacac cgaagggtga ccagtaccgc
     1141 ggcaaggtgt ggttgggcat caccacctcg gccgcggcgg gagccgtcgc cgcgtacgaa
     1201 cgctgcgcgt tggtgcgcgg gaacacctcg acgttgccgg gccacgccag cgcgatcccc
     1261 ggtccatccg acgcgacgcc gctggccgag ttcgtcgcga gcctgcccga gtgggacctg
     1321 tcacccttgc cgcgcaccga gtgggccacg ggcaccgccc tcagcgatcc gctgcgcgac
     1381 cacatcgacc tggcggcgcc gcttgcccgg tggagcttca accaggctgc cgtgcaccgc
     1441 gacgtcaccg cgacggccaa cggccgccgc ctcgtctacg ggggacacgt ccagggcttg
     1501 gcccaggcgt cgttgacccg catggctccg ggcgtcgcgc tcgtgctcgg ctgggacggc
     1561 tgcgaccaca tcggccctgc gttcgagggc gacctgctcg agttccgtca cgaggtgatc
     1621 gaagcactgc ccgccggcgg cggacgtttg ctgcgcctca gcgtcaccgg caccgtggtg
     1681 agcgacgacg gcgcccgcac cgacgtgctg cgctggacgc cgatcgtgtg gagcgcctga
     1741 gcgttcaggc tccgcactcg ccgcgcatca ggcgaccgct accgatcagg tgtctgcggg
     1801 gttgaactcg agcgcgaccg agttcatgca gtagcgcagc ccggtcggtg tgccataccc
     1861 gtcagggaag acgtgaccga ggtggctgtc gcagcgggcg cagcgcacct cggtgcgctc
     1921 catacccagg gtgtggtcgg ccagcagcgt cacggcttcc gggcggaccg actcgtagaa
     1981 gctcggccat ccgcccgact cgaacttggt gccgctcttg aacagctcgt tgccgcagcc
     2041 cttgcaggtg aacagtccgt cgcgcttctc cttcagcaat tcgccggtga aggcccgctc
     2101 ggtcgccgct tcgcgcagca cggcgtacga cagcgggtcc aggcgagtgc gccactcgtc
     2161 gtcggtgcgt tcgatctcag ccatgggtcg ctccttcata atcggttcgg cacagtcggt
     2221 tcggcatagt cggttcgtcg ccatcggttg tgtggccatc ggttgtgtgg ccatcgaggg
     2281 acgacggatc tacgggtgac gctaccccgg cccggcacaa cgcaaccgcg ggcgcccggc
     2341 agcggccgcc gtgcgcggtg ctacgtggcc agcgtccatt cggtgctgaa ctcgtcctga
     2401 ccggcgacga cgaagcgacg gccggtgacc gaggtgatgt cgagctccat ccactgatgc
     2461 cgttggatgc tgaccaacgg catcgtgtcg ggcaggtgct gcacgccgaa caaccgccgc
     2521 aggtgaccgc gggcgatcac gctccaggcc tgaccgcgcg ccggtgagac gtggtcgacc
     2581 tcgaagctgg ccgcgccttc gcaacgggtc atcgccgcgt tgggcgacgt gcggaagatg
     2641 atgcgcggcg tgccgaccgt ctgcaccagc cgatagttga cgggatacgc gatcgggtag
     2701 ccgcgatcga tcacgcacat ccgcccgaga ccctcgctgg acagcaggtc gtagcactcc
     2761 cacgcggcca gttcggccac cgccgccggt catcggccgc tgcggccgct ccccccgtcc
     2821 cggcccactc tggccatcgc cgtcgccggt gtgcttggcg tccggctcgg acgacggggc
     2881 gtcgggatcg ggcgtggcgt cgggatcggg caatggctcg tggcccggat cggcgccggt
     2941 gtgatgctcg ctcatggctc ctccagcgga cgtgatcgtc gcggggtcag gtgacgtcca
     3001 atcgccattg tggcaccaca tcaaccgccc gactcagggc cgttggtcag gtatggcggg
     3061 cgagccaggc gatcgtgtcg gcggtgacct cgtcgcggtt ggtctcgttg aggatctcgt
     3121 ggcgggcgtc ggggtacagc gtgaggtcgg cgctcacccc tgcggcgccg agcgcggcgg
     3181 cgagggtggc cgggaactcg cccatgcctg ccgcggggtc cttgtcgcct gcgatcacca
     3241 acaccgggca ggcgatcgag cccaacgact cggccgccgg gccgaccacg ccgaagatgt
     3301 cgatcaggta gccgaacgtg atcgggtagt cgtcgccgca gaacgggtcg gcggcatatg
     3361 cgtcgacctc ggcctcgtcg cggctcagcc agtcgtacgg ggtgcgggcc ggttcgaacg
     3421 cggcgttgtt cgcagcgagc acgggtgcgg ccgactcgtc acacagcccg gctgcatcga
     3481 ccgcagcgag catctcggcc aacccgcccg ggtcggccag attggcgggg aagccgcaca
     3541 ggatgccccc cgccaggccc gccgaatgct ggaccaggta ggccaacccg atcagcgacc
     3601 ccagcgagtg cccgaacagg aagaccgggg tctccgggcc aacctcggtc gaggtcagcg
     3661 tgcggagttg gtgcaggtcc tcgatcaccg ccgagccacc gccgggtccg gtgcggccca
     3721 cgcccgtcac tgcggcgctg ttgccgtgac cacggtgatc gagcgcggcg gccgcgtagc
     3781 ctgccgcgtt cagcgcccgg gcgaagcggt cgtaccgacc actgtgctcg gaagcaccgt
     3841 gagcgatcag taccagccct ttcgggtcgg ccaccggcca ggtgcgagtg aacacgtcga
     3901 tgccgtgtgt gtcggtgaaa gaaccctcgt gcatgcccgc agcgtaggcc cggcaccaac
     3961 ccgccatcgg agtcaggcgg ggtgcaggac gagggcttgt ttgatccagg cgagcaggcg
     4021 gtgggctggg agcgcgccga cctgacgctc gaccaccttg ccgccgcgca tcagcagcag
     4081 ggtggggatg ctctgcgcgc cgaaccgggc cgagatgccg ggcgcctcgt cgacgttgac
     4141 cttgaccgcc ttgagccgcc cggccaactc ggccgcagcc tgctcgacgc ctggcgccac
     4201 catgcggcac ggcccacacc acggcgccca caggtcgacc aacaccagcg ccgtcgtgtc
     4261 gactgccgcg tcgaagtcgg catcgccagc gttgaccagc cacgccaacg cggccttgca
     4321 cgacgcgcag cgcggcgtac cggcggctac caccggcacg cggttcttcg ttccgcacga
     4381 cgggcaggtg acgatctcgc tcatgactcg gctccgttca tctggtacac gctcatctgg
     4441 cgcagatggc gaggaacatc gggtactcgg cgccgtcacg cacgatctgg tggcagcgag
     4501 agaactcgac ctgatcgaac ccggcctccg cgagttggcg gcgcaggtcg tcgtgatcga
     4561 agccgtgatg gccgccgaag ccgccagggt ggaacgagcc gtcctccttg tcgaggtcga
     4621 cgatgcagag atgcccacca ggggtcaaca tctccgcgaa gccgctcagc accggggtca
     4681 ggtcggcgac gtggtgcagg gtcaagatgg tggcgatcag gtcgaatcgt gcttggccgg
     4741 gggcatcgtg cgagaggtcg agcgcgctga ccgtggcgtt gcggatcaac ccggcgtcga
     4801 tcttgccctg gatgaccgcc cgcattccgc tggacgagtc agccagcacg gccgacccga
     4861 cacggccacc gatcgcttgc gtgaacaacc cgctgcccgc gccgtagtcg agcagacgag
     4921 tcgaaccgtc gagcggaatc gccgccgcga tcgcgtccgc gacgacctgc gcccgcacga
     4981 cgtgatcggg attgtcgtcc cacgtcgccg cccgctcgtc gaagtggctc gccccgtact
     5041 cgtccccgtg atcgtggctg tggtgatcgt cgccgtgctg atcgtggctg tggtgatcgt
     5101 gactgtgcgc atgccgctgg ccgtgatcgg gcgtgtggct gtgcgtgtga tcgtgagcgt
     5161 ggccatcatc gtggccgtga tcgggagccg gtccggcggg atgttgctcg tcgtgcatgc
     5221 cgtcagtgtg ccgcgccggg cagggcggac aaggccgcgg tcaatcggcc tgcggcctcg
     5281 gtggccaact cggccatctc tgcgccgggc atcatctggc gcggatcggc ggcacggacg
     5341 cgggtaccac cttcgaccgg ctccaacacc acgttgcagg gcagcatcag cgccgcgtcg
     5401 gcatccgact ccagcgcccg atgggcgagg ttcgggttgc aggcgccgag gatcttcagc
     5461 ggcgggcgct cgatgccgag cttggcccgc aacacggtgg cgacgtcnnn nnnnnnnnnn
     5521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncggtgtt cgtgccgcgt
     5701 cgtgtgtcat cggcgccgct tcagccacga ccgcatccga cccgcgatgc cgcacgacac
     5761 accgtcttgc gatgtcaccg acgcggcaga agccggggcc ttgccggacc gcagtcgttc
     5821 ggcgcagtcg caccgttgat cgagcgccac atcgccgagc accatctcga cgtgccgacc
     5881 gcatccggca aag
//
LOCUS       DAORNF010000112         5888 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_10029_length_5888_cov_1.893880, whole genome shotgun sequence.
ACCESSION   DAORNF010000112 DAORNF010000000
VERSION     DAORNF010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 5888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5888
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10029_length_5888_cov_1.893880"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            113..>3376
                     /locus_tag="PLV68_05995"
     CDS             113..>3376
                     /locus_tag="PLV68_05995"
                     /inference="COORDINATES: protein motif:HMM:NF037016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HRE00830.1"
                     /translation="MRTEVESGKAVAYDVLADWWDPDGDDLQLLGATTAGKGAVSYSP
                     GGVVRYEAFGVSSGVETITVRVSDGHAQNSEANAELSVTVKPQGQPIKPNAKDDFVTV
                     LEGRTASVQLLVNDSDGNFKPGDPNARLTASLGDLSQLPGGITATYAASTDTLTVTGV
                     TPGVYAVPYTASDGTDTDTAIVRVEVLATPAVNQPPLAVPDRVVLRPGRIVNVDVLTN
                     DVDLDGDIVAVVSANAPTFEPAAGGVRATVIDRRLVQVELVAPTGTDALRGPFTISYT
                     IDDGNGTGDVSHRSIGALTVLVDNSIHDQAPVMVSDNLRVRTGDIGRVAVLANDSDPD
                     GDKIELTGLDPVDVTTFTTRGGGVAWVDKQTVRVRGGTPGQYVLHYTVVANGRAASGE
                     LNVTVTPDPTESSLNQAPTPRTVEVRVGRGNTTRIKVPLDDIDPDGDSVAIESVSLTE
                     QGTAGTTVSIDPGDPTRLLYTTSPVGATARDSFTYQVTDRLGQGATGTVQVLIVDVEP
                     SAPVAHDDVMRARPGRVVLVPVVSNDSDADDDPIALAELPFVDGEGQPTATPANPDLV
                     DVVRDVEGGLVTGGRLQVEVPAVGQPAVHERYRVTDPAGNVSNPANVSVIPDPEAPNL
                     APIAGTHVVEAGEVRGLDEITVDLSLDVSDPDDALGKLQIGLPDAVAMPGVQARVDGG
                     AVVVTLTESAQRVIYTVTDADPDNPLTSHGIIEVPGKQNHAPVLQQWVTVAGAIETES
                     SPVTPITLRLSEIVEDPDGDTGIVLTATPVDGGGYDVRRSDDNLSFTFTPSESTIAYD
                     VPIQFEVEDRPGDRTSLRATLTVTVHVTPGNTPPVFQGAGQVQLPQYREPVTYSLAGL
                     MRDADGDLLTYVLTNVDELRGITVNQDGDSITLEGTDSSIAPGQSYTLLFTATDGIGE
                     HQPVDGQVRITIVTTNKGEPVAQDFPNLTAVRDEPANPVDVIAAAVNPHASTGDPLRL
                     QSVRVTGAASLNCTTACGQQPIVFTASQPGTFTVTYVIEDVVGNTATGTLTYLVRGRP
                     LVPGTPEVTSVGDRTVNLSWSAADDQGSPITKYVVRAVEAGITHETTTTSIAFDGLTN
                     "
     assembly_gap    3377..3392
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3393..4977
                     /locus_tag="PLV68_06000"
     CDS             <3393..4977
                     /locus_tag="PLV68_06000"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HRE00831.1"
                     /translation="VYAVNAIGDGVVSGTSSIGIPDRVPSPPQNLRITDYGNQTLTLQ
                     WDAPSDAGDYSTISAYEINLAGFGLISVDGGTLQRVVTGLTNGTDYTFTVRARNAATS
                     NNGWGGSSGSSVSERPSTYPGAPLSVTAATAGDGGQARVRVDWAAPSDNGGREVTSYR
                     VCSVQSPSDCQDVTVRTATFNVTTGSDNSYTVIAFNSDKNRNNSDVSAASNVVRGIVT
                     PGIPTINSVTSGNGTLTVSATPGSNGGCSTTYIQYIRTGTSTWQESNVFTGLANGTEY
                     TITARMSLPSSCQTPSGVARSSDSSNAVAQTPYGPIATPTINGSASGQTLSFSWNANQ
                     SANGRQWTVSVWPNSSGGPLDGCYWSSNYQGSATGNCSGNVGYSYSETVTIQVCATVG
                     GTECRSAQATPATGPPPTPVVTIGLGGMTTSGGCAAGDGQVYDSQGCYFIAVSLSNSS
                     SGSHTIHCYHSTNAGLWAEFDSYSTTNTSSQGCSYSSAGRWVTVVVDGTGSGSSPGSP
                     PTPSSGGVVSNIYGQWPTN"
     gene            5012..>5888
                     /locus_tag="PLV68_06005"
     CDS             5012..>5888
                     /locus_tag="PLV68_06005"
                     /inference="COORDINATES: protein motif:HMM:NF040105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HRE00832.1"
                     /translation="MTPEQAAWYADAFAKLVANVERAVVGKTHTIRLALTTLLADGHL
                     LLEDVPGTGKTMLAKALANTVQGTQNRVQFTPDLLPSDVTGVTIYDQRSGQFTFHDGP
                     VFCTVLLADEINRASPKTQSALLEVMEENRVTVDGVAHPVPAPFMVIATQNPIEQAGT
                     YRLPEAQLDRFMMRTSLGYPDHENTVALLADAANRHRESGVQPLITTKAVVEMAQLAT
                     GVHVDPAVLSYMTRIAEETRRAPEVKLGVSVRGLLAYVRAVKAWAASNGRNYVVPDDV
                     KELAEPLMAHRIILEP"
BASE COUNT         1056 a         2062 c         1890 g          864 t
ORIGIN      
        1 ccacgccgca cccagcgcgc cgccaaggca gagcgccacg cacaaggcca cgcccgcggc
       61 gaggaagatg tcgagcgcga ccacgcgatc ggcatggcgc gggccgcgca cgatgcgcac
      121 cgaggtcgaa agcggtaagg cggtcgccta cgacgtcctg gccgactggt gggaccccga
      181 cggcgacgac ctccaactcc tcggtgcgac caccgccggc aagggagcgg tcagctactc
      241 gcccggtggc gtcgtgcgct acgaggcctt cggcgtgtct tcgggggtgg agacgatcac
      301 cgtgcgggtc agcgacggcc acgcccagaa cagcgaggcc aacgccgagt tgagcgtcac
      361 cgtcaagccg cagggtcaac cgatcaagcc caacgccaag gacgacttcg tgaccgtgct
      421 ggagggtcga accgcttcgg tgcagttgct cgtcaacgac tccgacggca acttcaagcc
      481 cggcgacccc aacgcccggc tcaccgcatc gctcggcgat ctgtcgcaac tacccggcgg
      541 catcaccgcc acctacgccg cgtcgacaga caccctcacg gtcacgggtg tcacccctgg
      601 tgtctacgcc gttccctaca ccgcgtcgga cggcaccgat accgacacgg ccatcgtgcg
      661 ggtcgaggtg ctggcgaccc ctgccgtcaa ccagccgccg ctggcggtgc ccgatcgggt
      721 ggtgttgcgc ccgggccgca tcgtcaacgt cgacgtgttg accaacgacg tcgatctcga
      781 cggcgacatc gtggccgtcg tctctgccaa cgcgccgacg ttcgagcccg cggcgggtgg
      841 tgtgcgcgcc accgtgatcg atcgtcggtt ggtgcaagtg gaactggttg cgccaaccgg
      901 gaccgatgcg ctgcgcggcc cgttcacgat cagctacacg atcgacgacg gcaacggcac
      961 cggcgacgtc agtcaccgtt cgatcggtgc cctgaccgtg ctggtcgaca actcgatcca
     1021 cgaccaggca ccggtgatgg tgtccgacaa cctgcgcgtc cgcaccggtg acatcggccg
     1081 ggtggcggtc ctcgccaacg actcggatcc cgacggcgac aagatcgagc tcaccggtct
     1141 cgaccccgtc gacgtgacca ccttcaccac gcgaggtggc ggggtggcct gggtcgacaa
     1201 gcagaccgtg cgcgtccgcg gcggtacacc cggccagtac gtgctgcact acaccgtggt
     1261 cgccaacggc cgtgccgcct cgggcgagct caacgtcacg gtcacacccg atccgaccga
     1321 atcgtcactg aatcaggcgc ccacgccgcg cacggtcgag gtgcgggtcg ggcgtggcaa
     1381 caccacccgt atcaaggtgc cgctggacga catcgatccc gacggcgact cggtggcgat
     1441 cgaatcggtc agcctcaccg aacagggcac ggcgggcacc acggtgtcga tcgaccctgg
     1501 cgacccgacc aggctgttgt acaccacgtc gccggtcggt gccaccgcgc gcgactcgtt
     1561 cacctatcag gtcaccgacc gtctcggtca gggtgcgacc ggtaccgtgc aggtgctgat
     1621 cgtcgacgtc gagcccagcg cgccggtggc tcacgacgac gtgatgcgcg cccgtcccgg
     1681 ccgggtggtg ctggtgcccg tggtcagcaa cgattccgac gccgacgacg acccgatcgc
     1741 gttggccgag ttgcccttcg tcgacggcga ggggcagccg acggccacgc ccgccaaccc
     1801 cgatctggtc gacgtggtgc gcgacgtcga aggcggcctg gtgacgggtg gacgattgca
     1861 agtggaagta cctgcggtgg ggcaaccggc agtgcacgag cgttaccgcg tcaccgatcc
     1921 ggcgggcaac gtcagcaacc cggccaacgt gtcggtgatc cccgaccccg aggcaccgaa
     1981 cctggctccg atcgcgggca cccacgtggt cgaggctggc gaggttcgtg ggctggacga
     2041 gatcacggtc gacctgtcgc tcgacgtgag tgacccggac gatgcgctgg gcaagttgca
     2101 gatcgggctt cccgacgcgg tggcgatgcc aggtgtgcag gcacgcgtcg acggcggcgc
     2161 tgtggtggtc accctgaccg agtccgcgca gcgggtgatc tatacggtca ccgacgccga
     2221 ccccgacaac cccttgacct cgcacggcat catcgaggtg cccggcaagc agaaccacgc
     2281 cccggtcctg cagcagtggg tcaccgtggc cggtgcgatc gaaaccgagt ccagcccggt
     2341 cacgccgatc accttgcgcc tgtccgagat cgtcgaagac cccgacggtg acaccggcat
     2401 cgtgctgacc gcgacccccg tcgacggcgg cggctacgac gtacgtcgca gcgacgacaa
     2461 cctgtcgttc acgttcacac cgtccgaatc gaccatcgct tatgacgtac cgatccagtt
     2521 cgaagtcgag gatcgcccgg gagaccgcac ctcgctgcgg gcgacgttga ccgttaccgt
     2581 gcacgtcacg ccgggcaaca cgccgccggt gttccagggc gccggtcagg tgcagttgcc
     2641 gcaataccgc gagccggtca cctattcgtt ggccgggttg atgcgcgatg ccgacggtga
     2701 cctgttgacc tacgtgctca ccaacgtgga cgagttgcgc ggcatcaccg tcaaccagga
     2761 cggcgattcg atcacgctgg aaggtaccga cagcagtatc gcgccgggtc agagctacac
     2821 cctgctgttc actgccaccg acggcatcgg cgagcaccag ccggtcgatg ggcaggtgcg
     2881 gatcaccatc gtcacgacca acaagggcga gccggtcgca caggacttcc ccaacctcac
     2941 cgcggtgcgc gacgaaccgg caaatccggt cgacgtcatc gccgcggctg tcaaccctca
     3001 cgcgtccacc ggcgacccgt tgcggttgca atctgtgcgt gtcacgggcg cggcatcgct
     3061 caactgcact acggcctgcg gccagcagcc gatcgtgttc accgcatccc agccgggcac
     3121 cttcaccgtc acatacgtga tcgaagacgt ggtcggcaac accgccacgg gcacgctcac
     3181 ctatctggtc aggggtcgtc cgctggtacc gggcacgccc gaggtcacca gcgtgggcga
     3241 ccggacggtc aacctctcct ggtcggccgc cgacgatcaa ggttcgccga tcaccaagta
     3301 cgtcgtgcgc gcggtcgagg cgggcatcac ccacgagacg accaccacga gcatcgcgtt
     3361 cgacgggctg accaacnnnn nnnnnnnnnn nnggtgtatg cggtcaacgc gatcggcgac
     3421 ggtgtggtca gcgggacgtc gagcatcggc attcccgacc gggtgcccag cccgccgcag
     3481 aacctgcgca tcaccgacta cggcaaccag accctgaccc tgcagtggga tgcgccgtcc
     3541 gacgcgggcg actacagcac catcagcgca tacgagatca acctggccgg gttcgggctg
     3601 atcagcgtcg atggggggac actgcagcgg gtggtcacgg ggttgacgaa cggcaccgac
     3661 tacacattca ccgtgcgagc ccgcaacgcg gcgacgtcga acaacgggtg gggtgggtcg
     3721 agcggatcat cggtgtccga gaggccgtcc acatatccgg gtgcgccgtt gtcggtcact
     3781 gccgcgaccg ctggcgacgg tggccaggcc cgcgtgcggg tcgactgggc cgcgccttcc
     3841 gacaacgggg ggcgcgaggt caccagctat cgggtgtgct cggtgcagtc gcccagcgac
     3901 tgtcaggacg tcacggtgcg cacggccacg ttcaacgtga cgaccggctc cgacaactcg
     3961 tacaccgtga tcgccttcaa cagcgacaag aaccgcaaca actccgacgt cagcgcggcc
     4021 tccaacgtcg tgcgcggcat cgtcacccct ggcatcccca cgatcaacag cgtgacctcc
     4081 ggcaacggca cgctcacggt ctctgccaca cccgggtcca acggggggtg ttcgaccacc
     4141 tacatccagt acatccgcac cggtacgtcc acgtggcagg agagcaacgt gttcaccggt
     4201 ctcgccaacg gcaccgagta cacgattacc gcgcgcatgt cgttgccgtc gagttgccag
     4261 acgccgagcg gtgttgcccg ctccagcgat tccagcaacg ccgtcgcaca aaccccgtat
     4321 gggccgatcg ccacgccgac catcaacggc agcgccagcg gccaaaccct gtcgttcagt
     4381 tggaacgcca accagtccgc gaacggacgc cagtggacgg tctcggtgtg gccgaactcg
     4441 tcggggggac cgctcgacgg ttgctactgg agcagcaact accaaggctc ggcgacgggg
     4501 aactgcagcg gcaacgtcgg ctacagctac tccgagaccg tcacgatcca ggtctgcgcc
     4561 accgtcggcg gcaccgagtg ccggagtgcc caggccacgc cagccaccgg tccgccgccg
     4621 acgccggtgg tgacgatcgg tctcggcggc atgacgacat ccggcggatg cgcggctggg
     4681 gacggacagg tctacgactc gcagggttgt tacttcatcg ccgtgtcgct ctcgaactcc
     4741 tccagcggtt cgcacacgat ccactgctac cacagcacca acgcggggct ctgggccgag
     4801 ttcgactcct actccacaac caacacctcc agtcaaggct gttcctactc gtccgccggt
     4861 cgatgggtga ccgtcgtcgt cgatggaacc ggtagcgggt catcgccggg ttcaccgccg
     4921 acgccgagct ccggcggcgt ggtgtcgaac atctacgggc aatggcccac caactgaggc
     4981 agatcgagat ggaccggaag gacagaacgg aatgacacct gaacaagcgg cttggtacgc
     5041 ggacgcgttc gccaagttgg tcgccaacgt cgaacgagcg gtggtgggca agacccacac
     5101 gatccggctc gccctgacga cgctgttggc cgacggccac ctgctgttgg aggacgtgcc
     5161 gggcactggc aagacgatgc tcgccaaggc actggccaac accgtgcagg gcacgcagaa
     5221 ccgggtgcag ttcacccccg acctgttgcc cagcgacgtc accggcgtga cgatctacga
     5281 ccagcgcagc ggacagttca cgttccacga cggtccggtg ttctgcaccg tattgctggc
     5341 ggacgagatc aaccgtgcct caccgaagac gcagtccgca ctgctggagg tgatggaaga
     5401 gaaccgggtc accgtcgacg gcgttgccca cccggtgccc gcgccgttca tggtgatcgc
     5461 cacccagaac ccgatcgagc aagccggcac ctaccgcctg cccgaggcgc aactcgaccg
     5521 gttcatgatg cgaacctcgc tcggctatcc cgaccacgag aacaccgtcg ccctgctggc
     5581 cgacgcagcc aaccgccacc gtgagtccgg tgtgcagccg ctgatcacca ccaaggcggt
     5641 ggtcgagatg gctcaactcg caaccggcgt gcacgtcgac cccgccgtgc tgtcgtacat
     5701 gacccgcatc gccgaagaga cccgccgcgc ccccgaggtc aagctcggtg tcagcgtgcg
     5761 cggcctgttg gcctacgttc gtgcggtcaa agcgtgggcc gcgtcgaacg gacgcaacta
     5821 cgtggtgccc gacgacgtga aggagttggc cgagccactg atggcccacc gcatcatcct
     5881 cgaaccgg
//
LOCUS       DAORNF010000113         5865 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_10084_length_5865_cov_1.434596, whole genome shotgun sequence.
ACCESSION   DAORNF010000113 DAORNF010000000
VERSION     DAORNF010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 5865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5865
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10084_length_5865_cov_1.434596"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>379)
                     /locus_tag="PLV68_06010"
     CDS             complement(<1..>379)
                     /locus_tag="PLV68_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HRE00833.1"
                     /translation="LNRLTRAGVLVEDRLFATLDPTTRRLALPGGEPVLLTDTVGFVR
                     RLPHGLVQAFKSTLEVAHSADFLIHVVDASSPDPGGEMIAVRDVLAEIEADQVPELVV
                     FNKADKDPDSAAELVAGHPGSVAV"
     assembly_gap    380..389
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<390..1064)
                     /gene="hflX"
                     /locus_tag="PLV68_06015"
     CDS             complement(<390..1064)
                     /gene="hflX"
                     /locus_tag="PLV68_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HRE00834.1"
                     /translation="MTNPYNEALGATLIERTKREKIVLVGVGLPGHSDDEVDASLDEL
                     ALLIDTAGADEAARLTQRRDAPDHTWYIGKGKVEELRDLCLAVDADTVVFDNELAPAQ
                     QYNLEKKLGRTALDRTAVILDIFAQNAHTLEGKAQVELALLRYRLPRLRRGVGGNLSQ
                     QGAGIGTRGPGETKLEVDRRRIMRRIAKLERDLRELQQTRDLQRKSRGDSGLAAVTIV
                     GYTNAGK"
     gene            complement(1061..>1318)
                     /locus_tag="PLV68_06020"
     CDS             complement(1061..>1318)
                     /locus_tag="PLV68_06020"
                     /inference="COORDINATES: protein motif:HMM:NF013813.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00835.1"
                     /translation="PGPEPSAIRMRVHERGAGVTEACGTGACASAWAAVQWGLAAPLD
                     GEIVVHMDGGDAKVRLHQPEPGHVTLVGPSVFIARLQIDMP"
     assembly_gap    1319..1486
                     /estimated_length=168
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1487..1895)
                     /locus_tag="PLV68_06025"
     CDS             complement(<1487..1895)
                     /locus_tag="PLV68_06025"
                     /inference="COORDINATES: protein motif:HMM:NF013813.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00836.1"
                     /translation="MTVTLTKYHGLGNDFLVCDTREVPVDADWVALARAWCERRRGMG
                     ADGLLSLTLLGDAATTTELAMVLHNADGSRAEMSGNGIRCLVHAAFIAQQRNEPVTYR
                     VHTDAGLRSVDVVPDDTPGQATTAPVTSVHASVA"
     gene            complement(1892..>2086)
                     /locus_tag="PLV68_06030"
     CDS             complement(1892..>2086)
                     /locus_tag="PLV68_06030"
                     /inference="COORDINATES: protein motif:HMM:NF013845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HRE00837.1"
                     /translation="YKELFAYLDGDLSLTDAVRDIVVRTQQYAVRQERWFRRDPRIRW
                     IDIVADPVAEVTPVIQELLS"
     assembly_gap    2087..2112
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2113..>2352)
                     /locus_tag="PLV68_06035"
     CDS             complement(<2113..>2352)
                     /locus_tag="PLV68_06035"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF013845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA dimethylallyltransferase"
                     /protein_id="HRE00838.1"
                     /translation="VRALEVCLGSGRPFSSFGPGVAAHAAESGGASGSAVAQIGLRWP
                     RPVLRQRIAQRVERMIDAGLVDEVRAVRARPGGVSR"
     assembly_gap    2353..2485
                     /estimated_length=133
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2486..2804)
                     /locus_tag="PLV68_06040"
     CDS             complement(<2486..2804)
                     /locus_tag="PLV68_06040"
                     /inference="COORDINATES: protein motif:HMM:NF013845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HRE00839.1"
                     /translation="MGPTASGKSDVAMAVAAGRADVEIVAVDAMQVYRGMDIGTAKPA
                     GADRAAVVHHGLDLVEPSESFSVAEYQRVATEALAAIAGRGHLALLVAGTGLYLAAVV
                     DGLT"
     gene            complement(2897..>4238)
                     /gene="miaB"
                     /locus_tag="PLV68_06045"
     CDS             complement(2897..>4238)
                     /gene="miaB"
                     /locus_tag="PLV68_06045"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HRE00840.1"
                     /translation="ADGLVLAESEADADVVVVNTCCIREGADTKTYGNLGHLKSWKDA
                     APGRQLVVAGCMAQKDRDAVRERAPWVDVVLGTHNVHRAGALIDTARTTGQAITEIFD
                     EAVIDDQAMFPSALPVRRDSGYNAWVTIQIGCDNSCAFCIVPAVRGAEISRPFADVVA
                     EVEQLAANGVSEVTLLGQNVNSYGRDLTLAARRTGADDVRIRPLFADLLRAVGAVPGI
                     RRVRFTSPHPKDMRGDTIAAMAETAAVCEHLHYPLQSGSDTVLAAMHRGYSAERYLRQ
                     LADARAAMPDLAVSTDIIVGFPGETDDDFAATLDVAAAAEYDYAYTFIFSPRPGTEAA
                     MFTDRFVAPAVAAERFERLKIVVERSALHKHRARVGRIEELLIEGPSRKDPGVMTGRT
                     RQNKLVHFPSPAPLRAGAYASVEIVSAAPHHLGGRLVEVLADAQHKIRIPVAVG"
     assembly_gap    4239..4329
                     /estimated_length=91
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4439..5029
                     /locus_tag="PLV68_06050"
     CDS             4439..5029
                     /locus_tag="PLV68_06050"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRE00841.1"
                     /translation="MGNRRDVGSAVERVRLVYAADHARLWRSIYAFSASADVADEATA
                     EAFAQVLRRGEEVRDVAAWVWRAAYSIAQGELQRRRAESQIRLAPQHVDGDERDARSN
                     RATPSAEDAHGDLAPVLAALATLPERDRQLVVMCHFAGWKPRELTEAFGASAATLRVR
                     LHRSTRRVRDLLISDAADSAIATTRATTTDDGRPDE"
     gene            5022..>5555
                     /locus_tag="PLV68_06055"
     CDS             5022..>5555
                     /locus_tag="PLV68_06055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00842.1"
                     /translation="MSDPIDQARERLAHVEVPNRWDEIVARAAVPGDTGADFGTRPVN
                     MPPRRTRGMVMWGTAAAAAVVALTVGTLAIRSGPAIAPGSTDPPATPTSATTPPDDTV
                     QPGNTTPTEPAPVTSTTSAATTTTAATTNTTAVVNDQPMWRRPCTDVSGTADSNPADE
                     FSSQTFGPLDHNPGIQIV"
     assembly_gap    5556..5645
                     /estimated_length=90
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5646..>5865
                     /locus_tag="PLV68_06060"
     CDS             <5646..>5865
                     /locus_tag="PLV68_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00843.1"
                     /translation="SPYTAMAVVNDDGQLRWRRCLDGESGWPQVFDAERGKIYGQFWN
                     RQHAASEWWEFDVADGTSNVLADDLVPRA"
BASE COUNT          841 a         1851 c         1850 g          805 t
ORIGIN      
        1 accgcgaccg aacccggatg accggcgacg agctcggccg ccgagtctgg gtccttgtcg
       61 gccttgttga acacgaccag ctcgggcact tggtcggctt cgatctcggc cagcacgtcg
      121 cgcaccgcga tcatctcgcc tcccgggtcg ggcgaactgg cgtcgacgac gtggatcaag
      181 aagtcggccg agtgggccac ttcgagcgtg ctcttgaacg cctgcaccaa gccgtgcggc
      241 aggcggcgga cgaagccgac ggtgtcggtg agcagcaccg gttctccgcc gggcagggcc
      301 aggcggcgcg tggtcggatc gagcgtggcg aacagccggt cttcgaccag cacacctgcc
      361 cgggtcaggc ggttgagcan nnnnnnnnnc ttacccgcgt tggtgtatcc gacgatggtg
      421 accgcggcca gcccgctgtc accccgcgac ttgcgttgca agtcacgggt ctgttgcagc
      481 tcgcgcaggt cgcgttcgag cttggcgatg cggcgcatga tgcggcggcg gtcgacttcg
      541 agcttggtct cgccgggacc gcgggtaccg atgccggcac cctgctggct gaggttgccg
      601 ccgacaccgc ggcgcaaacg cggcaggcgg tagcgcagca gcgccagttc gacctgggcc
      661 ttgccttcga gcgtgtgagc gttctgggcg aagatgtcga ggatgaccgc ggtgcgatcg
      721 agcgcggtgc ggccgagctt cttttcgagg ttgtactgct gggccggagc gagttcgttg
      781 tcgaacacca cggtgtcggc atcgacggcc aagcacagat cgcgaagttc ttcgaccttg
      841 cccttgccga tgtaccacgt gtgatcggga gcatcgcgcc gctgggtgag gcgggccgcc
      901 tcgtcggcac cggcggtgtc gatcagcagg gccaactcgt cgaggctggc atcgacctcg
      961 tcgtcgctgt ggccggggag gccgacgccc acgaggacga ttttctcgcg cttggtgcgc
     1021 tcgatcagcg tggcgccgag ggcttcgttg tacggattgg tcatggcatg tcgatctgca
     1081 ggcgggcgat gaacaccgac ggcccgacca gggtcacatg ccctggttca ggctgatgca
     1141 gacgcacctt tgcatctccg ccatccatgt gcacgacgat ttctccatcg agcggggccg
     1201 ccaggcccca ctgcacggcg gcccaggccg acgcgcacgc gccggtgcca cacgcttcgg
     1261 tgacgcccgc gccgcgttcg tgcacccgca tccggatggc cgacggctcg gggcccggnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntggc cacgctggca
     1501 tgcaccgacg tcaccggcgc ggtcgtggcc tgccccggcg tgtcgtcggg aaccacgtcg
     1561 acagagcgca gcccggcatc ggtgtgcacc cgataggtga cgggctcgtt gcgctgttgg
     1621 gcgatgaacg cggcatggac gaggcaacga atgccgttgc cgctcatctc ggcccggctg
     1681 ccgtcggcgt tgtgcaacac catcgccagc tcggtcgtgg tcgcagcgtc gcccagcagt
     1741 gtcagcgaca gcagtccgtc ggcccccatg ccgcggcgcc gttcgcacca cgcccgggcc
     1801 agcgccaccc aatcggcatc gacgggaacc tcgcgcgtgt cgcacacgag gaagtcgttg
     1861 ccgagtccgt ggtacttggt gagggtgacg gtcatgacag caactcctgg atcacgggcg
     1921 tcacctcggc gaccggatcg gcgacgatgt cgatccagcg tatgcgtggg tcacggcgga
     1981 accaccgctc ttggcgcacg gcgtactgct gggtgcgcac gacgatgtcg cgcaccgcgt
     2041 cggtcaggct caggtcgccg tcgaggtagg cgaacaactc cttgtannnn nnnnnnnnnn
     2101 nnnnnnnnnn nncgcgagac cccaccgggc cgcgcccgca ccgcgcgaac ctcgtcgacc
     2161 agcccggcgt cgatcattcg ctcgactcgc tgcgcgatgc gttggcgcag caccgggcgg
     2221 ggccaacgca acccgatctg ggcgacggcg gacccgctgg ccccgccaga ctcggcggca
     2281 tgggctgcga cgcccgggcc gaagctgctg aacgggcggc cgctgccgag gcagacctcc
     2341 agggcgcgca cgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnngggtc aacccgtcga cgaccgcggc cagatacagc
     2521 cccgtccctg cgaccagcag agcgagatgg ccacgcccgg cgatggcagc gagcgcttcg
     2581 gtcgccaccc gctggtactc ggccacgctg aacgattcgc tcggctcgac caggtcgaga
     2641 ccgtgatgga cgaccgcggc ccggtcggcc ccagcaggct tggccgtgcc gatgtccatg
     2701 ccgcggtaca cctgcatcgc gtcaacggcg acgatctcga cgtcggcccg ccccgcggca
     2761 accgccatcg cgacgtcgct cttgccgctg gcggtcgggc ccacaatcac aacctggcgg
     2821 gggatcacga cctggcgggc acggcccacg gagtcagcgc tgagatcgtc gccggccacc
     2881 gctacctcgg cgggtttcaa ccgaccgcga cagggatgcg gatcttgtgc tgcgcgtcgg
     2941 ccagcacctc gaccaggcga ccaccgaggt ggtggggagc agcgctgacg atctcgacgc
     3001 tcgcgtaggc acccgcccgc agcggggccg gcgagggaaa gtggacgagc ttgttctgcc
     3061 gcgtgcggcc ggtcatcacg ccgggatcct tgcgcgacgg cccttcgatc aacagttcct
     3121 cgatccgtcc gacccgcgcc cggtgcttgt gcagcgcact gcgctcgacc acgatcttca
     3181 accgctcgaa ccgttctgcc gcaacggcgg gggcgacgaa ccggtcggtg aacatcgctg
     3241 cctcggtgcc gggacggggc gagaagatga acgtgtaggc gtagtcgtac tcggccgccg
     3301 cggcgacgtc gagcgtggcc gcgaaatcgt cgtcggtctc gccaggaaag ccgacgatga
     3361 tgtcggtgct gaccgcgagg tcgggcatcg ccgcgcgggc gtcggccagt tggcgtaggt
     3421 agcgctcggc gctgtagccg cggtgcatcg cggccagcac cgtgtcggag ccggactgca
     3481 gcgggtagtg gaggtgctcg cacaccgctg ccgtctcggc catggccgct atggtgtcac
     3541 cgcgcatgtc cttggggtgc gggctggtga agcgcacccg gcgaatgccc ggcaccgcgc
     3601 ccaccgcgcg cagcaggtcc gcgaacagcg gacggatgcg cacgtcgtcg gcacccgtgc
     3661 gccgcgccgc aagggtgaga tcgcgcccgt agctgttgac gttctgcccg agcagggtca
     3721 cttcggacac gccgttcgcg gcgagttgct cgacctcggc cacgacatcg gcaaacggac
     3781 ggctgatctc ggcgccgcgc accgcaggca cgatgcagaa cgcgcacgag ttgtcgcagc
     3841 cgatctggat ggtgacccac gcgttgtatc cgctgtcgcg ccgaaccggc agtgcgctgg
     3901 ggaacatcgc ctggtcgtcg atgaccgctt cgtcgaagat ctcggtgatc gcctggccgg
     3961 tggtgcgggc ggtgtcgatc agcgcgccgg cccggtgcac gttgtgcgtg ccgagcacga
     4021 cgtcgaccca cggtgcccgc tcgcgcacgg cgtcgcgatc cttctgggcc atgcatccag
     4081 cgaccaccaa ctggcgcccc ggggcggcgt ccttccaact cttgaggtgg ccgaggttgc
     4141 cgtaggtctt ggtgtcggcg ccttcgcgaa tgcagcaggt gttgacgacc acgacgtcgg
     4201 cgtcggcctc agactcggcc agcaccaatc catcggctnn nnnnnnnnnn nnnnnnnnnn
     4261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnnnnc agagcatgct aatggccctc cccgctcccc acccccgacg aaatccgcac
     4381 ccgcctgtaa cgatccggcc cgaacagccc ctgtcgccgt gtggtggaat cggcaagcgt
     4441 ggggaacaga cgagacgtcg gtagcgctgt cgagcgcgtc cgtctcgtct atgccgccga
     4501 ccatgctcgc ctgtggcgct cgatctacgc attcagcgcg tcggcggacg ttgctgacga
     4561 agcgaccgcg gaggccttcg cccaggtgct gcgccgcggt gaggaggtcc gcgacgtcgc
     4621 cgcgtgggtc tggcgcgctg cgtacagcat cgctcagggc gagttgcagc gacggcgcgc
     4681 cgaatcccag atccgcttgg caccgcaaca cgtcgacgga gacgaacgag acgctcgcag
     4741 caacagggcg acgccatcgg ccgaggacgc ccacggcgat ctggcaccgg tgcttgccgc
     4801 gctggcgacc ttgcccgaac gcgaccgcca actggtcgtg atgtgtcact tcgccgggtg
     4861 gaaaccacgt gagttgaccg aggcgttcgg ggcctcggcc gcgacgctgc gcgttcgcct
     4921 gcaccgctcg acccgccgag tacgcgactt gctgatcagc gacgcggccg actcggcgat
     4981 cgcaaccacg cgtgcaacca cgaccgacga cgggaggccc gatgagtgat ccaatcgacc
     5041 aggcccgtga gcgtctagcc cacgtcgagg tacccaatcg gtgggacgag atcgtcgctc
     5101 gcgcggcggt gcccggcgac acgggcgccg acttcggaac tcggccggtg aacatgcctc
     5161 cgcggcggac tcgcgggatg gtgatgtggg ggacggctgc ggccgcggct gtggttgcac
     5221 tgacggttgg gacgctggcg atcaggtcgg ggccagcgat cgcgccggga tcgactgatc
     5281 caccagccac gccgaccagc gcgacaacgc cccccgacga caccgtgcag cccgggaaca
     5341 caacgccgac cgaacccgca ccggtcacgt cgaccaccag tgccgccacg accacgaccg
     5401 cggcgacaac caacacgacc gcggtcgtca acgaccaacc gatgtggaga cgcccgtgca
     5461 cggacgtctc cggcacagcc gacagcaacc cagccgacga gttcagcagc cagaccttcg
     5521 gcccactcga ccacaacccc ggtatccaaa tcgtcnnnnn nnnnnnnnnn nnnnnnnnnn
     5581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5641 nnnnncgagt ccgtacaccg cgatggcggt cgtcaacgac gacggccagt tgcgctggcg
     5701 gcgatgcctt gacggcgaga gcggctggcc ccaggtgttc gatgccgaac gggggaagat
     5761 ctacggacag ttctggaacc gacagcacgc cgcgtcggag tggtgggagt tcgacgtcgc
     5821 cgacggcacg tcgaacgttc tcgccgacga tctcgtcccc cgagc
//
LOCUS       DAORNF010000114         5864 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_10086_length_5864_cov_1.969874, whole genome shotgun sequence.
ACCESSION   DAORNF010000114 DAORNF010000000
VERSION     DAORNF010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 5864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5864
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10086_length_5864_cov_1.969874"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1242
                     /locus_tag="PLV68_06065"
     CDS             <1..>1242
                     /locus_tag="PLV68_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HRE00844.1"
                     /translation="DRLVCGDVGFGKTEVAVRAAFKAIQDGKQVAVLAPTTLLATQHG
                     NTFADRFAGFPIRVEVLSRFLTAGQAKQVIEGLKSGEVDCVIGTHRLLQESVMFKDLG
                     LLIVDEEQRFGVQHKEAMKKLKVNVDVLTLTATPIPRTLEMSLVGIRDLSLLQTPPAD
                     RQPILTFVGEYDERVAIESIRRELLREGQVFWVHNRVQSIDQCASRLRELVPEARIAV
                     AHGQMDEGTLEQVVLDFSEGKLDVLVCTTIIESGIDMPTVNTLVVERADLLGLGQMHQ
                     LRGRVGRSGSRAYAYLFYPPDKSLTEEAYERLKTIGEATDLGSGFKIAMRDLEIRGAG
                     NLLGQAQSGHIAAVGYDLYCQMVTEAVSEMKGEPQKAPNEVKLDVPTDAHLPASYVPK
                     ESLRLEAYRRLAAIETDADVAD"
     assembly_gap    1243..1252
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1253..1566
                     /locus_tag="PLV68_06070"
     CDS             <1253..1566
                     /locus_tag="PLV68_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00845.1"
                     /translation="AEWEDRYGPLPEAAEALLVVGQLRAECNRLGITEITITSNQARM
                     APLTLRTSEELRLRRLARDAIMKDDVKQLVVPIRRGVEPATFLVNFLRELIPAPVPAP
                     A"
     gene            1650..2573
                     /locus_tag="PLV68_06075"
     CDS             1650..2573
                     /locus_tag="PLV68_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00846.1"
                     /translation="MSPRRRRFSVATLASLTAVAALTTGCATFTGNKHAASVNGEHLA
                     ITDFEAMLVDAPNLGLQDSDTSGEYSGDTARALLGVWVSATAISTGLAERGLTISDDD
                     RAVAQARYSSSDPATWEKLSVPTKELLINFTAARTALANDPSLASAARTLYEDGVTGS
                     GVLCLRIMAFDTEDEATAALEQVRNGTDFAAIAEDHPVDPSAPANGGIFTDQSTGAEC
                     LDASGYQPLAQALLDVPVGESSEVTTLGSTFFFLILQRPYDEVADQLASLLGTAGAQA
                     LAESITSAADVSIDSRYGQWDAESGEVVAVR"
     gene            2570..>2750
                     /locus_tag="PLV68_06080"
     CDS             2570..>2750
                     /locus_tag="PLV68_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00847.1"
                     /translation="MTDAARPRIVVVGLGPGGPEYVTEHTRAAIAAAPHRYLRTTQHP
                     SASLVPDAISFDAVYD"
     assembly_gap    2751..2810
                     /estimated_length=60
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2811..4077
                     /gene="mazG"
                     /locus_tag="PLV68_06085"
     CDS             <2811..4077
                     /gene="mazG"
                     /locus_tag="PLV68_06085"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HRE00848.1"
                     /translation="HGEVLYAVPGSPLVLERTVDLLRADDRVEITALAAMSFLDLAYA
                     ALGIDPVEAGLRLVDGHTFATAAAGERGPLLIAHTHANWVLGEIKLAADDASADNDDA
                     EVVILHHLGTPEQQIVHTTWAEMDSAIEADHLTSVYVPRLNVAVAEHYARFHALTRTL
                     REQCPWDVEQTHQSLIPYLLEETYEVVDALQALDASDPSSDVDLIEELGDLLYQIEFH
                     ATIAEQQGRFTIADVTQGVHDKLVRRHPHVFPPAGQDDVIGVDGTSQVLANWETIKRT
                     EKQRTSIFDGIPGSMPSLSYAYKVQKKAAGVGFDWPNAEGALAKIGEEASEVVAAAQA
                     GDADHDDAATRRERVADEVGDLLFSVVNVARHLKVDPEVALRAAVGKFRSRFEAVEQL
                     ATQRGIDLSAAGLTVLDQLWDEVKARQVQ"
     gene            complement(4083..>4699)
                     /locus_tag="PLV68_06090"
     CDS             complement(4083..>4699)
                     /locus_tag="PLV68_06090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00849.1"
                     /translation="HDLRVAAASIAAVALAVHAMYVVWAIVAARNAHRRSILAPTPWA
                     VIGAFLSGPVIVGISASLSGPFGAAVLTIGVTASIAGFIVGQLVLGRTVAALDGRGRI
                     FTHWLGVDILTGVLVSITSLIELRPLETVILAVVQTAVVLLNATLAWNAMSTLDRTCR
                     AYRYGGSTVAATTLPTPLASGANPITGPISAVALEPITVGSSAL"
     assembly_gap    4700..4770
                     /estimated_length=71
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4771..>5069)
                     /locus_tag="PLV68_06095"
     CDS             complement(<4771..>5069)
                     /locus_tag="PLV68_06095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00850.1"
                     /translation="ALPLDDPQHVLRVRAFACYLSAMMLAASAALVSIAASNLQARIA
                     HRWQREIDPVGADNDPSRLKIARSGRQLRRRFVPTLFLRVLVTAGLKGGEQIVVQG"
     assembly_gap    5070..5126
                     /estimated_length=57
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5127..>5405)
                     /locus_tag="PLV68_06100"
     CDS             complement(<5127..>5405)
                     /locus_tag="PLV68_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00851.1"
                     /translation="AVMASWSFIVVANVNRVGHSLRSAWFAALGWLLAPGLGLAVHVT
                     IDRYLDSGSLAGITVFLAVLYIPFGTIGGATHDLGGSAHFARTWFSASV"
     assembly_gap    5406..5546
                     /estimated_length=141
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5754..>5864
                     /locus_tag="PLV68_06105"
     CDS             5754..>5864
                     /locus_tag="PLV68_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00852.1"
                     /translation="MSEILSVVGREILDSRGNPTVEVDVLLTSGAFGRAAV"
BASE COUNT          960 a         1955 c         1822 g          788 t
ORIGIN      
        1 gaccggctgg tgtgcggcga cgtcggtttc ggcaagaccg aggtcgcggt gcgggccgcg
       61 ttcaaagcaa ttcaggatgg caagcaggtg gccgtgctgg cccccaccac gctgctcgcg
      121 acccagcacg gcaacacctt cgccgaccgc ttcgccgggt tcccgatccg ggtcgaagtg
      181 ctcagccgct tcctcaccgc cggccaggcc aagcaggtga tcgaggggct gaagagcggc
      241 gaggtcgact gcgtgatcgg cacccaccgc ctgctgcaag agagcgtcat gttcaaggat
      301 ctcggcttgc tgatcgtcga cgaggagcag cgcttcggcg tgcagcacaa ggaggcgatg
      361 aagaagctga aggtcaacgt cgacgtgttg accctgaccg cgacccccat cccgcgcacg
      421 ttggagatga gcctggtcgg tatccgcgac ctgtcgctac tgcagacgcc accggccgac
      481 cgccagccga tcctgacctt cgtcggcgaa tacgacgagc gggtggcgat cgagtcgatc
      541 cgccgcgagc tgctgcgtga aggccaagtg ttctgggtgc acaaccgcgt ccagtcgatc
      601 gaccagtgcg cgtcgcgcct gcgcgagctc gtgcccgagg cgcgcatcgc cgtcgcccac
      661 gggcagatgg acgagggcac actggaacag gtggtgctcg acttctccga gggcaagctc
      721 gacgtgttgg tgtgcaccac catcatcgag agcggcatcg acatgcccac ggtcaacacg
      781 ctggtggtcg agcgggccga cctgctcggc ctgggtcaga tgcaccagct acgcggccgc
      841 gtgggccgca gcgggtcgcg cgcctacgcc tacctgttct acccacccga caagtcgctg
      901 accgaagagg cctacgaacg gctgaagacc atcggcgagg ccaccgactt gggcagtggg
      961 ttcaagatcg cgatgcgcga cctggagatc cggggcgcgg gcaacctgct cggccaggcc
     1021 cagagcggtc acatcgccgc ggtcggctac gacctgtact gccagatggt gaccgaagcg
     1081 gtcagcgaga tgaagggcga gccacagaag gccccgaacg aggtcaagct cgacgtgccg
     1141 accgacgccc acctgcccgc cagctacgtg cccaaggaga gcctgcgcct ggaggcctac
     1201 cgccgcctcg cggcgatcga gaccgatgcc gacgtggccg acnnnnnnnn nngcgccgag
     1261 tgggaagacc gctacggccc gctgcccgag gctgccgagg cgctgctggt ggtcggccag
     1321 ttgcgcgccg agtgcaatcg gctcggtatc accgagatca cgatcacgtc gaaccaggcc
     1381 cggatggctc cgctcacgct gcgcaccagc gaggagctgc ggctgcgccg cctggcccgc
     1441 gacgcgatca tgaaggacga cgtgaagcaa ctggtcgtgc cgatccgccg cggcgtcgag
     1501 cccgccacct tcctggtgaa cttcctgcgc gagctgatcc cggcaccggt gccggccccc
     1561 gcctgagccg tcgccggcgc tcggacccac atctgcgcca cccgcagcaa caggcccgcg
     1621 gcgggtgacg ccaggtagcg ttgccagccg tgagcccacg ccgccgtcgc ttctccgtcg
     1681 ccaccctcgc cagcctcacc gcggtcgccg cattgaccac cggctgtgcc accttcaccg
     1741 gcaacaagca cgccgcatcg gtcaacggcg agcacctcgc catcaccgac ttcgaggcca
     1801 tgctggtcga cgctccgaat ctcggcctcc aagactccga cacgtcgggt gagtactcgg
     1861 gtgacacagc gcgcgcgttg ctcggcgtct gggtgtcggc caccgcgatc tccaccgggc
     1921 tcgccgagcg tggcttgacg atttccgacg acgatcgtgc cgttgcccag gcccgctaca
     1981 gctcatccga cccggcgacc tgggagaagc tctcggtgcc gaccaaggag ctgctgatca
     2041 acttcacggc cgcgcgcacc gcgctggcca acgacccgtc gctggcgtcg gctgcgcgca
     2101 ccttgtacga ggacggtgtg accgggtcgg gggtgctgtg cctacggatc atggccttcg
     2161 acaccgagga tgaagcgact gcagcgttgg agcaggttcg caatggcacg gacttcgccg
     2221 ccatcgccga agaccacccg gtcgatccga gcgcgccggc caacggcggc atcttcaccg
     2281 atcagtccac gggggccgaa tgcctcgatg catccggcta ccagccgctg gcccaagccc
     2341 tgctcgacgt ccccgtcggc gagtcgtcag aggtcaccac cctgggcagc acgttcttct
     2401 tcctgatcct gcaacggccc tacgacgagg tggccgacca gttggcgtcg ctactcggca
     2461 ccgccggtgc gcaagcgttg gccgaatcga tcacctccgc tgccgacgtc tcgatcgaca
     2521 gccgctacgg ccagtgggac gctgagagcg gtgaggtcgt ggccgtccga tgacggacgc
     2581 tgcccggccc cgcatcgtcg tcgtcgggct tggccccggt gggcccgagt acgtcaccga
     2641 gcacacccgg gccgcgatcg ctgccgcgcc gcatcgctac ctgcgcacca cccaacaccc
     2701 gtcggcctcg ctggtgcccg atgcgatcag tttcgatgcg gtctacgacc nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn acacggcgag
     2821 gtgctgtacg cggtgcccgg ctcaccgctg gtgctggaac gcaccgtcga cctgctgcgc
     2881 gccgacgacc gcgtcgagat caccgcgctt gccgcgatgt cgttcctcga cctggcctat
     2941 gccgcgctcg gcatcgaccc ggtggaagcc ggactgcggt tggtcgacgg gcacaccttc
     3001 gccaccgctg cggcgggcga gcgcgggccg ctgctgatcg cgcacaccca tgccaactgg
     3061 gtgctgggtg agatcaagct ggccgccgac gacgccagcg ccgacaacga cgacgcagag
     3121 gtcgtgatcc tgcatcacct cggcacgccc gagcagcaga tcgtccacac cacctgggcc
     3181 gagatggact cggccatcga agccgatcac ctcacatcgg tctacgtgcc gcgcctgaac
     3241 gtcgccgtgg ccgagcacta cgcccgcttc cacgccctga cccggacgct gcgagagcag
     3301 tgcccgtggg acgtcgagca gacccaccag tcgctgatcc cgtacctgct cgaagagacc
     3361 tacgaggtgg tcgacgcgct ccaggcgctc gacgcatccg accccagcag cgacgtcgac
     3421 ctcatcgaag agctcggcga tctgctctac cagatcgagt tccacgccac gatcgccgaa
     3481 cagcagggcc ggttcaccat cgccgacgtc acccagggcg tgcacgacaa gctcgtgcgg
     3541 cgccatccgc acgtgttccc acccgctggc caggacgacg tgatcggtgt cgacggcacc
     3601 agccaggtac tggccaactg ggagacgatc aagcgcaccg agaagcagcg gacgagcatc
     3661 ttcgacggca tccccggctc gatgccgtcg ctgtcgtacg cgtacaaggt gcagaagaag
     3721 gccgccggcg tcggcttcga ctggcccaac gccgagggcg cgctggccaa gatcggtgaa
     3781 gaggcgtccg aggtcgtcgc tgcagcgcaa gccggcgacg ccgaccacga cgatgccgcc
     3841 acgcgccgcg agcgggtcgc cgacgaggtg ggcgacctgt tgttctcggt ggtcaacgtc
     3901 gctcgccatc tcaaggtcga tcccgaggtc gccctgcgcg ccgcagtcgg caagtttcgc
     3961 agccgcttcg aagccgtcga gcagttggca acccagcgcg gcatcgatct gtccgccgcc
     4021 ggtctgaccg tgctcgacca actgtgggac gaggtcaaag cccgccaggt ccagtgacga
     4081 cgtcagagtg cagatgaccc gacggtgatc ggctccaagg cgaccgccga gatcggcccg
     4141 gtgatcgggt tggcaccgga tgcgagcggg gtcggcaacg tggttgcagc taccgtcgag
     4201 ccgccatagc gataggcgcg acaggtgcga tcgagggtgc tcatcgcgtt ccacgccaac
     4261 gtcgcgttca gcagcaccac ggccgtctgc accaccgcga ggatcaccgt ctccaacggc
     4321 cgcaactcga tcaggctggt gatgctgacc agaacaccgg tcagaatgtc gacgcccaac
     4381 cagtgggtga agatccggcc ccgtccgtcg agcgcggcca ccgtgcggcc caacaccagt
     4441 tggccgacga tgaagcccgc gatcgacgcc gtcacgccga tcgtcagcac ggcggcgccg
     4501 aaggggccac tcaggctggc gctgatgccc acgatcaccg gtccgctcaa gaacgcgccg
     4561 atcacggccc acggcgtcgg cgcgaggatc gaacggcgat gggcattgcg ggcggcgacg
     4621 atggcccaca cgacgtacat cgcgtgcacc gccaacgcga ccgcggcgat cgaggccgcg
     4681 gcgacgcgca gatcgtggan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ccctgcacga caatctgctc gccgcccttc
     4801 aggccggcgg tgaccagcac gcgcaggaac agcgtgggca cgaaccggcg gcgcagttgg
     4861 cggcccgagc gggcgatctt caaccgcgac ggatcgttgt cggctccgac cgggtcgatc
     4921 tcacgctgcc aacgatgggc gatgcgggcc tgcagattgc tggccgcgat cgacaccagc
     4981 gcggccgacg cggcgagcat catcgcgctg agatagcagg cgaaggcccg cacccgcagc
     5041 acgtgctgcg ggtcgtcgag gggtagggcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5101 nnnnnnnnnn nnnnnnnnnn nnnnnnaccg acgccgagaa ccacgtgcgg gcgaagtggg
     5161 ccgacccgcc gaggtcgtgc gtcgctccac cgatggtgcc gaacgggatg tacagcacgg
     5221 cgaggaagac ggtgatgcca gccaacgaac ccgaatcgag atagcgatcg atcgtgacgt
     5281 gcacagccaa ccccaaccct ggagcgagca gccagccgag cgcggcaaac caggccgagc
     5341 gcaggctgtg ccccacccga ttgacgttgg cgacgacgat gaagctccaa ctcgccatca
     5401 ccgccnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5521 nnnnnnnnnn nnnnnnnnnn nnnnnngcgg taccaccacg atcagccagc gcagcaacag
     5581 gttggcgatg ggctgacggg cgacgcggcg cgtcacggtc gcaacttcca ccgggttcac
     5641 cccatctcgt tcgtcagcca cgggtgctct atcggcagcg accagccagc gcttgagcac
     5701 gggcccgcaa ccgtttgggt taccgcggag gggccggaac tatcgttgcg gccatgtctg
     5761 agatcctctc cgttgtgggc cgcgagattc tcgattcgcg cggcaacccc accgtcgaag
     5821 tcgacgtgct gttgacgtcc ggggccttcg gccgcgccgc cgtc
//
LOCUS       DAORNF010000115         5832 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_10153_length_5832_cov_1.851307, whole genome shotgun sequence.
ACCESSION   DAORNF010000115 DAORNF010000000
VERSION     DAORNF010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 5832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5832
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10153_length_5832_cov_1.851307"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>5832)
                     /locus_tag="PLV68_06110"
     CDS             complement(<1..>5832)
                     /locus_tag="PLV68_06110"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HRE00853.1"
                     /translation="PVESRPAPTAANRVDQAFPAADDSDPPEVVSGPLHVVRHQPDGD
                     VGIAPFVTITFDQPMVAVGTSGPLRPAEVPVTITPAVEGAWQWIGTRPLRFDASVAGI
                     DRLPMATEYAVVVPAGTTSATGNALEADYSFRFNTPPASVQSFWPSTGDDTQITPTQL
                     FLAAFDQRVDAEAVVAHATISAKGNSYPLRVATAAEVAADETVSRLVADAPDGRWVAF
                     RAVDPLPLDTRFTVTFGTGLPSAEGPRPSTAEQSFRVQTYGPLTLRKASCDWGNSECR
                     PGSSLVFEFTNELDAKRFDPATVSIEPAIPGAVVGVSGSALTVWGGTAGSTTYTVSVP
                     AGLVDIYGQTLAEGVSKAFEIGPAQPALRDFASPLVTIDPNAPKPHVDIVTVNHDSVD
                     VSVFAVQPGDWGAFAQYLMNEVYSYRPDRTLPDPSFTRLAQRTVAIDNQPDRATITSI
                     DLSQELTAPTGHVVVLVRPSASAGNSGTDYDNMARVAWVQATQLNVDAFFDQRNLVAW
                     TTELTTGSPVADVAVQVLDGYGAVKVDPATTDGEGLARFTLPVTDDVRESRELPTVLV
                     ATKGDDTALLPSADSAWGGGWWGGRQDDQALWYVVDDRQVYQPGETVSVSGWVRRFTT
                     ASDAQVVRVPASATVTWQAHDGQGNTIADGTATLNPLGAFSLQFDVPADSNLGYASIN
                     LALNGAPSLPFASTGHQFRIEQYRRPEFEVSARPESAEPHVVGTPLTVAVDAAYYAGG
                     PLPDAPVTWNVTTNGATYVPPGWERYTFGRWTPWWRSPYGGRSTPAIDDSCCWDNPTT
                     TATFTGRTNGAGSDYLQIDVSGLAPDQAGYPIAVRTQASVTDVNRQAWAANTTQVVHP
                     GEVYVGLAGEQTFVELGQPIEVDAVAVGIDGGVAAGRTITITATRQTTVFRNGRYETT
                     DADPQVCTVTSAAEPVRCSFDTPLPGSYTVRAEVADAAGRISRSEITRWVSGADHTSI
                     DRVRGEQLTLVPDADTYAAGSTARLLVQSPVAEGTGLLVVSRNGIRSTDTFTVTGGSA
                     VVEVPLADRDIPSVDVVIEVAGTSPRNDDGSVTRPAYATGQITLPISLASRTLTVAAT
                     PHDAVTEPGATTAVDVTVTDAAGKPVAGADFTVMVVDEAVLALSGTSLADPLASFYPG
                     LYSWVSTRFGRDHVALVDLATLTNVSPDGQTTADSGVVATTTAPAATVFESAAEQSVD
                     TAGGATGAPPPNASRQSAGDGDDSFAQYKVSADGSPIDVRANFTPIAVFAPSQTTGAN
                     GSATIQVPLPDSVTRYRVMVVAAAGDAQFGSTTASVTARLALTVRPSAPRFANFGDTF
                     ELPVVLQNQTDGTLAVDVALQTDNLATTGPAGLRVEVPANNRVEVRFGVGTEAVGTAR
                     FRIAAASGQLADAATVTLPVYTPATAEAFATYGVIDGTGSVTATAQPIESPTDVFAQF
                     GGLEVSTSSTALAALTDAAIAVTDYPYNSSDAAASRILTIVALRDVLDAFGSPNLPSR
                     AKLDAAINADIARITALQNGDGGFAYWERGKDSSPFNSVHALHALLLARDAGYSVQRG
                     TIDSALWYLQAIEGALHDYSADARDSVIAYALFTRDLADDRDASRAEQLVTSRNDLPL
                     DALAWAWPVVDSDSVSRRIATTVANRAVDTAATVNFVTAPSDSDVLTLRSDRRTDALV
                     LDSLIREQPTSDMIPKVVAGLLAGQRTGRNGGQWDGIQENTFILLALHRYFATYEGTD
                     PAFVASVWLGDRFAGSHEFTGRSTDQVTVVVPTAALIEQLGTTDDDMLTIANDGAGRL
                     YYRIAMRTAPTDLDLDPLDRGFTVSRTYEAVDDPADVSQDTDGTWRIRAGARVRVRVT
                     MVAESQRAHVALIDPLAAGTEILNPDFATTPDLPQDDNGGVTPYPIDTFDAYGGSAAR
                     GWYDWFWRSTWYDHQNRRDDRAEAFATWLSAGVYDYSYVVSATT"
BASE COUNT          825 a         1856 c         2252 g          899 t
ORIGIN      
        1 ggtggtggcc gacacgacgt acgagtagtc gtacaccccg gcggacagcc acgtggcgaa
       61 ggcctcggcc cggtcgtcac ggcggttctg atggtcgtac cacgtcgagc gccagaacca
      121 gtcgtaccac ccgcgcgcgg ccgagccccc gtacgcgtcg aacgtgtcga tcgggtacgg
      181 cgtcacgcca ccgttgtcgt cctggggcag atcgggcgtc gtcgcgaagt cggggttgag
      241 gatctcggtc cccgcggcga gcgggtcgat cagggcgacg tgggcacgct ggctctcggc
      301 gaccatcgtg acccgcaccc gcacgcgggc accggctcgg atgcgccagg tgccgtcggt
      361 gtcctggctg acgtcggccg ggtcgtcgac ggcttcgtac gtgcgactga cggtgaagcc
      421 ccggtcgagg ggatcgaggt cgaggtcggt cggcgcggtg cgcatcgcga tgcggtagta
      481 caggcgcccg gcgccgtcgt tggcgatcgt cagcatgtcg tcgtcggtgg tgcccaactg
      541 ctcgatcagc gccgcggtcg gcaccaccac ggtcacctgg tcggtcgagc ggccggtgaa
      601 ctcgtgcgat ccggcgaagc gatcgcccaa ccagaccgag gcgacgaagg ccgggtcggt
      661 gccttcgtag gtggcgaagt agcggtgcag ggcgagcagg atgaacgtgt tctcctggat
      721 gccgtcccac tgtccgccgt tgcggccggt gcgctgcccg gcgagcagac cggcgaccac
      781 cttggggatc atgtcgctgg tcggttgctc gcggatcagc gaatcgagca ccaacgcatc
      841 ggtgcgccgg tcggaacgca gcgtcaagac gtcgctgtcg cttggggcgg tgacgaagtt
      901 gacagtcgcc gcggtgtcga ccgcgcggtt ggcgacggtg gtggcgatgc ggcgcgacac
      961 gctgtcggag tcgaccaccg gccacgccca cgccagcgcg tcgaggggca ggtcgttgcg
     1021 gctggtgacc aactgctcgg cgcggctggc gtcgcgatcg tccgcgagat cgcgggtgaa
     1081 cagcgcgtag gcgatgacgc tgtcgcgggc gtcggcgctg tagtcgtgca gcgcaccctc
     1141 gatcgcttgc aggtaccaca gcgccgaatc gatggtgccg cgctgtacgg agtacccggc
     1201 gtctcgggcc agcagcaggg cgtgcagcgc gtgcaccgag ttgaacggcg aactgtcctt
     1261 gccccgctcc caataggcga aaccgccgtc gccgttctgc agcgcggtga tcctggcgat
     1321 gtcggcgttg atcgcggcgt cgagcttggc cctgctgggc aggttgggcg agccgaaggc
     1381 atcgagcacg tcgcgcaggg cgacgatggt gaggatgcgc gatgcggcgg cgtcgctgga
     1441 gttgtacgga tagtcggtga ccgcgatcgc cgcgtcggtc agcgcggcca gcgcggtgga
     1501 cgaggtggaa acctccaacc cgccgaactg cgcgaacacg tcggtgggcg actcgatcgg
     1561 ctgagcggtg gccgtgaccg aaccggtgcc gtcgatgacc ccgtaggtgg cgaacgcctc
     1621 cgccgtggcc ggggtgtaca ccggcagggt cacggtggcc gcgtcggcca actggccgct
     1681 ggcggccgcg atacggaagc gagcggtgcc gaccgcttcg gtgccgacgc cgaagcgcac
     1741 ctcgacccgg ttgttggccg gtacctcgac ccgcagcccg gccgggccgg tggtggccag
     1801 gttgtcggtc tgcagagcga cgtcgacggc gagcgtgccg tcggtctggt tctggagcac
     1861 caccggcagt tcgaacgtgt cgccgaagtt ggcgaaccgc ggcgcggagg ggcgcacggt
     1921 cagcgccagg cgggcggtga ccgaggcggt ggtcgagccg aactgggcgt cgcccgcggc
     1981 cgcgacgacc atcacccggt agcgggtcac gctgtcgggc agcggcacct ggatggtggc
     2041 cgagccgttg gcgccggtgg tctggctggg ggcgaacacg gcgatcgggg tgaagttggc
     2101 gcggacgtcg atcggcgaac cgtcggcgga caccttgtac tgagcgaacg agtcgtcgcc
     2161 atcgcccgca ctctggcggg atgcgttcgg cgggggggcg ccggtagcgc ctcccgcggt
     2221 gtccacgctc tgctccgcgg ccgactcgaa cacggtcgcc gctggtgcgg tcgtcgttgc
     2281 gacgacgcct gagtcggcgg tcgtttgtcc gtccggtgag acgttggtca gcgtggccag
     2341 atcgaccagg gcgacgtggt cgcgaccgaa tcgggtcgac acccacgagt acaggccggg
     2401 gtagaagctg gccagcggat cggccagtga cgtaccgctc agcgcgagca ctgcctcgtc
     2461 gacgaccatc acggtgaagt cggctccggc gacgggcttg cccgccgcgt cggtgacggt
     2521 gacgtcgacc gcggtggtcg cgcctggctc ggtgaccgca tcgtgcggtg tggccgcgac
     2581 tgtgagcgtg cgcgaagcga gcgagatcgg cagggtgatc tggccggtcg cgtaggccgg
     2641 gcgggtgacg ctgccgtcgt cgttgcgggg actggtgccg gccacttcga tcaccacgtc
     2701 gaccgatggg atgtcgcggt cggccaatgg cacctcgacc acggccgaac cacccgtgac
     2761 ggtgaacgtg tcggtcgagc ggatgccgtt gcgggacacc accagcaggc cggtaccttc
     2821 ggctacgggc gactgcacca gcaggcgagc ggtggaaccc gcggcgtacg tgtcggcgtc
     2881 gggcaccaac gtcagttgct cgccacgcac ccggtcgatc gacgtgtggt cggcaccgct
     2941 gacccagcgg gtgatctcgc tgcggctgat ccgtcccgcg gcgtcggcga cctcggcccg
     3001 cacggtgtac gaaccgggca ggggtgtgtc gaagctgcac cgcaccggct ctgcggccga
     3061 ggtcaccgtg cacacctgcg ggtcggcgtc ggtggtctcg tagcggccgt tgcggaacac
     3121 ggtggtttgg cgggtggcgg tgatggtgat cgtgcggccc gcggcaacgc cgccgtcgat
     3181 gccgaccgcg accgcgtcga cctcgatcgg ctggccgagc tcgacaaagg tctgctcgcc
     3241 cgcgaggccc acgtagacct cgccggggtg tacgacctgg gtcgtgttgg ccgcccacgc
     3301 ctggcggttg acatcggtga ccgacgcctg cgtgcgcacg gcgatggggt agcccgcctg
     3361 atcgggggcc aggccggaga cgtcgatctg caggtagtcg ctgcccgcgc cgttggtgcg
     3421 tccggtgaag gtcgccgtgg tggtcgggtt gtcccagcag caactgtcgt cgatggcggg
     3481 ggtggagcgg ccgccgtagg gcgagcgcca ccacggggtc cagcggccga acgtgtagcg
     3541 ctcccaaccg ggcggcacgt aggtggcacc gttggtggtg acgttccagg tgaccggtgc
     3601 gtcgggcagt ggcccacctg cgtagtaggc ggcgtcgacc gcgacggtca gcggcgtgcc
     3661 gaccacgtgc ggctcggcgc tctcgggccg ggccgacact tcgaactcgg gccgccggta
     3721 ctgctcgatg cggaactggt ggccagtact tgcaaatgga agtgatggtg ccccgttgag
     3781 cgcaaggttg atcgatgcgt agccgaggtt ggagtcggcg ggaacgtcga actgcaacga
     3841 gaaggcgccg agcgggttca gcgtcgcggt gccatcggcg atggtattgc cctggccgtc
     3901 gtgggcctgc cacgtcacgg tggccgaggc gggcacgcgc accacctggg catcgctggc
     3961 ggtggtgaac cggcgcaccc agccgctgac cgagacggtc tcgcccggtt ggtacacctg
     4021 gcggtcgtcg accacgtacc acagcgcctg gtcgtcctgg cgtccgcccc accagccacc
     4081 gccccaggcc gagtcggcgc tgggcagcag ggcggtgtcg tcgcccttgg tggcgaccag
     4141 cacggtgggc agttcacgcg actcgcggac gtcgtcggtg accggcaggg tgaagcgggc
     4201 gagaccttcg ccgtcggtcg tcgccgggtc gaccttgacc gcgccgtagc cgtcgagcac
     4261 ctgcacggcc acgtcggcaa cgggcgagcc cgtggtcagc tcggtggtcc aggcgacgag
     4321 gttgcgctgg tcgaagaagg cgtccacgtt gagctgggtg gcctgcaccc aggcgacgcg
     4381 cgccatgttg tcgtagtcgg taccggagtt gccggcgctg gcgctgggcc gcacgagcac
     4441 caccacgtgt ccggtgggcg ccgtcagctc ttggctgaga tcgatcgagg tgatcgtggc
     4501 ccggtcgggt tggttgtcga tcgccacggt gcgctgcgcg agccgggtga acgacgggtc
     4561 gggcagcgtg cggtcggggc ggtacgagta gacctcgttc atcaggtact gggcgaaggc
     4621 gccccagtcg ccgggctgca ccgcgaacac cgatacgtcg accgagtcgt ggttgacggt
     4681 cacgatgtcg acgtggggct tgggtgcgtt ggggtcgatg gtgaccagcg ggctggcgaa
     4741 gtcgcgcagc gccggctggg ccggaccgat ctcgaacgcc ttggacacgc cttcggccaa
     4801 cgtctggccg tagatgtcga ccagaccggc aggcacgctg acggtgtatg tggtgcttcc
     4861 ggccgtgccg ccccacacgg tgagcgcgga gcccgacaca ccgacgaccg cgccggggat
     4921 cgccggttcg atcgacacgg tcgccgggtc gaaccgcttg gcgtcgagtt cgttggtgaa
     4981 ctcgaagacc agcgacgagc cagggcggca ctccgagttg ccccaatcgc acgaggcctt
     5041 gcgcagggtc agcggcccgt acgtctgcac cctgaacgac tgctcggcgg tggaaggacg
     5101 cggcccttcc gccgacggca gcccggtgcc gaaggtgacc gtgaaccggg tgtcgagcgg
     5161 cagcgggtcg acggcgcgga acgcgaccca acgaccgtcg ggagcgtcgg cgaccaagcg
     5221 gctgacggtc tcgtcggcgg cgacctctgc cgcggtggcg actcgcaacg ggtagctgtt
     5281 gcccttcgcc gagatcgttg catgtgcaac gactgcctcg gcatcgacgc gctggtcgaa
     5341 cgcggccagg aacagttgag tcggtgtgat ctgtgtgtcg tcgcccgtcg agggccagaa
     5401 cgactgcacc gacgccggtg gggtgttgaa gcggaacgag tagtcggcct cgagcgcgtt
     5461 gccggtggcc gaggtcgttc cggccggaac gaccaccgcg tactcggtgg ccatcggcag
     5521 gcgatcgatg ccggcgacgg acgcatcgaa gcgcaggggg cgggtgccga tccactgcca
     5581 ggcaccctcg acggcaggtg tgatggtgac cggtacctcg gcaggccgca gcgggccgga
     5641 ggtgccgacg gcgaccatcg gctggtcgaa ggtgatggtg acgaacgggg cgatgccgac
     5701 gtcaccgtcg ggctggtggc gcacgacgtg cagcgggccg gacacgactt cgggcggatc
     5761 gctgtcgtcg gcggccggga aggcttgatc gacccggttc gccgcggtgg gggctggccg
     5821 tgactcgacc gg
//
LOCUS       DAORNF010000116         5785 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_10248_length_5785_cov_1.740663, whole genome shotgun sequence.
ACCESSION   DAORNF010000116 DAORNF010000000
VERSION     DAORNF010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 5785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5785
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10248_length_5785_cov_1.740663"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    322..350
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<351..1682)
                     /locus_tag="PLV68_06115"
     CDS             complement(<351..1682)
                     /locus_tag="PLV68_06115"
                     /inference="COORDINATES: protein motif:HMM:NF013225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HRE00854.1"
                     /translation="MSSTGLAAPLALRSRVPGAARVSIRALGERTVVFLDVDASHRRG
                     ALSSEASSMIAAAAIAAREQGLPLVAVMGSAGADIAEGFSALHGWGLAAKALTDCSGV
                     VPTIIVVNGPAVSGPALLLGIADLVVMTESAYAFVSGPTMVAEFTGVDIDNEELGGAA
                     SHARYSGAASLVAPDVDAALAMVEQLLSYLPDTVDQEPLLVPTDDPVERETPEAGTLI
                     PSASTGSYDVRHVIRALADDGDLLEVRARWAANVVTAFATFGGRPVGIVANQPISLAG
                     TLDIPASQKAARFVAFCDAFNLPIITMVDTPGFYPGKDLEWRGMIRHGAQLVFAYGRA
                     TVPRICLVLRKSYGGAYIVMDSKKMGNDLCLAWPWAELAVMGAGQAAAILQRRATPDE
                     RAAFEADYTERLLNPYVAAERGLIDGVVEPADTRREICNALAALADKRERLQ"
     gene            complement(1786..2724)
                     /locus_tag="PLV68_06120"
     CDS             complement(1786..2724)
                     /locus_tag="PLV68_06120"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRE00855.1"
                     /translation="MDLRQLATLVAIADHGTFSAAARAMFTVQSNISNHVGRLEKELG
                     VTLVDRQRGGLTDDGGQVVERARRILREVDDIAADMASRGDSVSGDARLGVIGTTARW
                     LMPQLLPALRVAHPFVHVTIYEGSTTNLIPRLMAGYLDGCIVHLPVDDPDLLVEPLFA
                     EDLIVLVHNHHEWAGEEELSLARVASVPLLLPPKGTAMRRILDRAAANANTSLKPQAE
                     IDGVRLMTSLAMEGYGPAIVPATSVPRWLKGDFTRIIVPELPRRVVGWVQRRRPLPSA
                     PGRAALHVMREVLQSSGANQHGIHVGPDAFPTAKTT"
     assembly_gap    2871..2903
                     /estimated_length=33
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2997..>3220
                     /locus_tag="PLV68_06125"
     CDS             2997..>3220
                     /locus_tag="PLV68_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00856.1"
                     /translation="MTKSASHGAASRGAAFFDLDRTLLSGASGEVFSAALRESGQVTR
                     SIPGQNLIFGLFNRIGETLPSMALARQAVTL"
     assembly_gap    3221..3230
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3231..>3765
                     /locus_tag="PLV68_06130"
     CDS             <3231..>3765
                     /locus_tag="PLV68_06130"
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HRE00857.1"
                     /translation="KSQADMRAMAQSAAPQLVARLQPFAAKLFAEHRAAGRPIVLATT
                     TPYDMVEPFADLLGFDGVVATRYGVNRKGLDELANLFTGKLQACRDWAERNGVSMDES
                     YAYSDSIYDTPLLSAVAHPVAVNPDPRLMVFARARRWKVMHLDVPAGVAKVPVLGIEL
                     QQLGMSFAHPALIPYAKF"
     assembly_gap    3766..3775
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3776..4446
                     /locus_tag="PLV68_06135"
     CDS             <3776..4446
                     /locus_tag="PLV68_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRE00858.1"
                     /translation="ISGTDNIPATGPAIVCGNHRSYFDPTAMALALARTGRPIRFLGK
                     KEVFDVPIVGRLAKAMGGIRVDRGTGSDEPLRAAAEALEGGQVVAIMPEGTIPRGMAF
                     FDPELKGRWGAAKLAAMTGSPVIPVGLWGTDKVWPRSARMPNVLNVTDPPLVTIRVGT
                     PVPMKYRSADADTKALMKAISALLPPEGRVKRTPTAEELAATLPPGYKGDPTAESKRR
                     PGTD"
     gene            4560..5009
                     /gene="aroQ"
                     /locus_tag="PLV68_06140"
     CDS             4560..5009
                     /gene="aroQ"
                     /locus_tag="PLV68_06140"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HRE00859.1"
                     /translation="MDRPIISLLHGPNLNLLGQREPAVYGTATLDDYVSATIAAAEPL
                     GFAVEWFQSNHEGALVDAIHAARGRAAAIVINPGAFTHYAWSLHDALAAFDGPIVEVH
                     ISNPNAREAWRHTSVVSPVATGTIVGLGMHGYELAVQAVARRLGGTP"
     gene            5075..>5785
                     /locus_tag="PLV68_06145"
     CDS             5075..>5785
                     /locus_tag="PLV68_06145"
                     /inference="COORDINATES: protein motif:HMM:NF013486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HRE00860.1"
                     /translation="MSATRQAGIEALFVTDLVNTRWLSGFSGSNGWLLVTADDVTLFT
                     DGRYGDQARHQLSAAGIEAEVSVGTTRAKMVGALAAAVSSRSISRLGFEADNLTVADH
                     AGFTAAVGTEWVATTGVVEPLRRCKDQGEIDRVARAAAIADDALGATLGLLSSEPTEA
                     SFRDALEAAMRAGGADGPSFDTIIAAGANAALPHHHPDHTRIVEGMTVVVDFGALYDG
                     YHSDMTRTFCIGDPTPMQA"
BASE COUNT          881 a         1975 c         1973 g          874 t
ORIGIN      
        1 gaggtaggtg atcgcgctct ggcacgcagc ggtctgcatg tacgcggggt cgtagatgaa
       61 cagcgacggg tcgagctcgc gcacggccga cgccgggaag actccgcccg tgatcggtac
      121 ggtgaccgtc ttgccggtgc ggtcgtcggt gatggtgatg gtctcgggca cgttgctccc
      181 ttggatgacg cggtcgtttg gcggggcttt ctccccgggc caggacacta ccggcctggt
      241 tcggagtgcg ccgaaagatg gcatcgctcg cgtcctggta ctaccagcac caccctcggg
      301 caccaccgcg agcgcggcgg gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ctggagacgc
      361 tcgcgcttgt cggccagcgc ggcgagggcg ttgcagatct cgcggcgggt gtcggccggt
      421 tcgaccacac cgtcgatcaa cccgcgctcg gccgcgacgt aagggttgag cagccgttcg
      481 gtgtagtcgg cctcgaacgc ggcccgctcg tcaggtgtgg cccggcgctg caagatggcc
      541 gctgcctggc ccgcccccat cacggccagt tcggcccacg gccaggccag gcacaggtcg
      601 ttgcccatct tcttgctgtc catcacgatg tacgcgccgc cgtagctctt gcgcagcacc
      661 aggcagatgc gcggcaccgt cgcccggccg taggcgaaca ccagctgggc gccgtggcgg
      721 atcatgcccc gccactcgag gtccttgccc ggatagaagc ccggggtatc gaccatggtg
      781 atgatcggca ggttgaacgc gtcgcagaac gcgacgaagc gggctgcctt ctgcgacgcc
      841 gggatgtcga gcgtgccggc cagcgagatc ggttggttgg cgacgatgcc caccggacgc
      901 ccgccgaagg tcgcgaatgc ggtgacgacg ttggccgccc agcgggctcg cacctcgagc
      961 aggtcgccgt cgtcggcgag ggcgcggatc acgtgacgca cgtcgtacga accggtcgac
     1021 gcggacggga tcagggtgcc cgcttcgggc gtttcccgct cgaccgggtc gtcggtggga
     1081 acgagcagcg gctcctggtc gacggtgtcg ggcaggtagc tgagcagctg ttcgaccatc
     1141 gccagggccg cgtcgacatc gggcgcgacc aggctggccg cgccgctgta gcgggcgtgg
     1201 ctggccgccc ctcccaactc ctcgttgtcg atgtcgacac cggtgaattc ggccaccatc
     1261 gtcgggccgc tgacgaaggc gtaggccgac tcggtcatca ccaccaggtc ggcgatgccg
     1321 agcaacaacg ccgggccgga caccgccggg ccgttgacga cgatgatcgt cggtacgacg
     1381 ccgctgcaat cggtgagcgc tttggccgcc agaccccagc cgtgcaacgc gctgaaccct
     1441 tcggcgatgt cggccccggc cgatcccatc acggccacga gcggcagacc ctgttcgcgg
     1501 gcggcgatcg ccgcggcggc gatcatcgac gaggcctccg acgacaaggc ccctcggcgg
     1561 tggctggcgt cgacgtcgag gaacacgacg gtccgctcgc cgagcgcacg gatcgagacg
     1621 cgggctgcgc ccggtacgcg ggatcgcaac gcaagcgggg cggcgagacc agtcgaagac
     1681 atcacggcga ctgtaacgcc ctggggtgga gcgccccgct tcggtcacgt cccaccagca
     1741 cactcgggtg gccgagctac cgagccggcg gtggggtttg ggcgctcagg tggtcttggc
     1801 ggtggggaac gcgtcggggc cgacgtggat gccgtgctgg ttggcgccgc tggattgcag
     1861 cacctcgcgc atcacgtgca gcgccgcccg accgggtgcg ctgggaagtg gccgccgccg
     1921 ctgtacccag ccgacgaccc ggcggggcag ctcgggaacg atgatgcggg tgaagtcgcc
     1981 cttcagccac cgcggcaccg aggtcgcggg cacgatcgca ggcccgtagc cctccattgc
     2041 caacgaggtc atcaggcgga ccccgtcgat ctcggcctgc ggtttgagcg aggtgttggc
     2101 gttggctgcg gcccgatcga ggatgcgccg catcgccgtc cccttgggcg ggagcagtag
     2161 cggcacgctg gcgacgcggg cgagggacag ctcttcttca ccggcccact cgtgatggtt
     2221 gtggaccagc acgatcaggt cctccgcgaa cagcggctcg accagcagat cggggtcgtc
     2281 gacgggcagg tggacgatgc atccgtcgag gtaaccggcc atcaggcggg ggatgaggtt
     2341 ggtcgtgctg ccctcgtaaa tggtgacgtg cacgaacggg tgggccacgc gcagcgccgg
     2401 caggagttgg ggcatcagcc atcgcgccgt ggtgccgatg acccccagcc gggcatcgcc
     2461 gctgaccgag tcaccgcggc tggccatgtc ggccgcgatg tcgtcgacct cgcgcaggat
     2521 gcgccgggct cgttcgacca cttgaccgcc gtcgtcggtc agcccgccac gctggcggtc
     2581 gacgagggtg actcccagct ccttttcgag ccgaccgaca tggttggaga tgttcgactg
     2641 caccgtgaac atcgcccggg cagccgccga aaaggtgccg tggtcagcga tcgcaaccag
     2701 cgtcgcaagc tgccgaaggt ccatctgtaa aaaagatagc tggtatctcc ccgttcgact
     2761 tgccagatcg catgttcacg tgcatacttt cacatggatc gcggcggtct cacaccccca
     2821 agctgaaccg cccgaccgaa cccatgcgat gttccccaag catctgggtt nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnccgcttc ccccaaggcg gggcctttcc cgttctctcc
     2941 ggcaccgcct cctgtccagc atcagccgct tcgggacgag ggcgtagtgt gccgccatga
     3001 cgaagtcagc aagccacggt gcagcgagca gaggagcggc gttcttcgac ctcgatcgga
     3061 cgttgctgtc gggagcctcc ggtgaggtgt tcagcgccgc gctacgcgag tccggccagg
     3121 tcacccgcag catccccggc cagaacctga tcttcggact gttcaaccgc atcggtgaga
     3181 cgctgcccag catggcgttg gcccgccagg ccgtcacgct nnnnnnnnnn aagtcgcagg
     3241 ccgacatgcg agcgatggcc cagtccgcag ccccgcagtt ggtcgcccgg ctccagccgt
     3301 tcgctgccaa gctgttcgcc gaacaccgcg ccgcgggccg cccgatcgtg ctcgccacca
     3361 cgaccccgta cgacatggtc gagcccttcg ccgatctgct cggcttcgac ggcgtggtcg
     3421 cgacccgcta cggcgtcaac aggaaggggc tggacgaact ggccaatctc ttcaccggca
     3481 agttgcaggc ctgccgcgac tgggccgagc gcaacggtgt ctccatggac gagagctacg
     3541 cctacagcga cagcatctac gacaccccgc tgctgtcagc cgtcgcccac ccggtcgcgg
     3601 tcaaccccga tccgcggttg atggtgttcg cccgggctcg gcggtggaag gtgatgcatc
     3661 tcgacgtccc ggcgggcgtg gccaaggtgc cggtgctcgg catcgagctg caacagctcg
     3721 gcatgtcctt cgcccacccg gcgctgattc cgtacgccaa gttcgnnnnn nnnnnacatc
     3781 agcggcaccg acaacatccc cgcgaccggc ccggccatcg tgtgcggcaa ccatcgcagc
     3841 tacttcgacc cgacggcgat ggcgctcgcc ctggcccgca ccgggcggcc catccgcttc
     3901 ctcggcaaga aggaggtatt cgacgtgccg atcgtcggcc gcctggccaa ggcaatgggt
     3961 ggcatccgcg tcgaccgcgg caccggcagc gacgaaccgt tgcgggccgc ggccgaggcg
     4021 ttggaaggcg gccaggtcgt ggcgatcatg cccgaaggca cgatcccgcg tggcatggcc
     4081 ttcttcgatc cggagctgaa gggccgctgg ggtgccgcca agctggcggc gatgacgggg
     4141 tcaccggtga tccctgtcgg gctgtggggc accgacaagg tgtggccgcg cagcgcccgg
     4201 atgccgaacg tgttgaacgt caccgatccg ccgctggtca cgatccgcgt cggcacaccg
     4261 gtgccgatga agtaccgcag tgccgatgcc gacacgaagg cgttgatgaa ggcgatcagt
     4321 gcgctgctgc cgcccgaagg ccgggtcaag cgcaccccga ccgccgaaga gctggccgcg
     4381 acgttgccgc cgggctacaa gggtgacccc accgccgaga gcaagcgacg acccggcacc
     4441 gactgaacac ggggtattgg ctgaacacgg ggtattggct gaacacgggc accggctgct
     4501 tgcccacgcg ctcgccgaac gtgctcgccg gttcgggcct gccctgccag actgtcgccg
     4561 tggatcgccc gatcatctcg ctgctgcacg gccccaacct gaacctgctc ggccagcgcg
     4621 agccggcggt gtacggcacc gccacgctcg acgactacgt gtcggccacc atcgccgcgg
     4681 ccgaaccgct ggggttcgcc gtcgagtggt tccagtccaa ccacgagggc gcgttggtcg
     4741 atgcgatcca cgccgcgcgc ggtcgcgctg cggcgatcgt catcaacccg ggcgcgttca
     4801 cgcactacgc gtggagcctg cacgacgcgc tggccgcgtt cgacgggccg atcgtcgagg
     4861 tccacatctc caacccgaac gcccgtgagg cgtggcggca caccagcgtg gtgtcgcccg
     4921 tggcgaccgg caccatcgtc gggctgggca tgcacggcta cgagctcgcg gtgcaggccg
     4981 tcgcccgccg cctcggtggc acgccgtgaa cacaggcttg gcgcccttcg accatccggg
     5041 ccggctcgcg cgcctccggg cagcgatcgc cgacgtgtcc gccacgcgac aggctggcat
     5101 cgaggccttg ttcgtcacgg atctggtcaa cacccgctgg ctcagcgggt tcagcgggtc
     5161 gaacggatgg ctgctggtca ccgccgacga cgtcacgctg ttcaccgacg gccgctacgg
     5221 cgaccaggcc cgccaccagt tgtccgccgc gggcatcgag gccgaggtct cggtcggcac
     5281 gacccgggca aagatggtcg gtgcgcttgc ggcagcggtg tcgtcgcggt cgatctcccg
     5341 gctcggcttc gaagcggaca acctcaccgt tgccgaccat gccggcttca cggcagcggt
     5401 cggtacggag tgggtggcca ccactggggt ggtcgagccg ttgcggcgct gcaaggacca
     5461 gggcgagatc gaccgcgtcg cccgtgccgc agcgatagcc gacgacgcgc tgggcgcgac
     5521 gctcggcttg ttgagcagtg aaccgaccga ggccagcttc cgagacgccc tcgaagcggc
     5581 gatgcgcgcc ggcggtgcag atgggccgag cttcgacacg atcatcgctg cgggtgccaa
     5641 tgcggcgttg ccgcaccacc atcccgacca cacccgcatc gtcgagggca tgaccgtggt
     5701 ggtcgatttc ggcgccctct acgacggcta ccacagcgac atgacccgca cgttctgcat
     5761 cggcgatccc acgccgatgc aggcc
//
LOCUS       DAORNF010000117         5753 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_10324_length_5753_cov_1.624781, whole genome shotgun sequence.
ACCESSION   DAORNF010000117 DAORNF010000000
VERSION     DAORNF010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 5753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5753
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10324_length_5753_cov_1.624781"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="PLV68_06150"
     CDS             complement(<1..399)
                     /locus_tag="PLV68_06150"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HRE00861.1"
                     /translation="MRTSTTSGRVTSPIRLGIRFDLRVPSFATVDHRTQYAAALDMAE
                     WAEGLGFRFVALSEHHGTPDGYLSAPFTLAAAILGRTKRIKINVAAALVPLHDPVRLA
                     EQLATIDLIGPGRFSFVAGAGYRREEFDMAG"
     assembly_gap    634..657
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <658..>1408
                     /locus_tag="PLV68_06155"
     CDS             <658..>1408
                     /locus_tag="PLV68_06155"
                     /inference="COORDINATES: protein motif:HMM:NF014789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRE00862.1"
                     /translation="GLGWIAGPPEYGGRGLPKQFSRLYNELEEGFATADQMRLSVGLG
                     MVGPTIAAHGSAEVKNRYLRSIYRGDVLACQLFSEPNAGSDLSAIRTRAERDGDSWRI
                     TGQKVWTSGGHFSDIGMVLARTGTDDSRTRGLTMFLIDMHALGVEVRPLRQMTGHASF
                     DEVFLDDVLTPDSNRLGQVDDGWSVMMTTLLNERGSIGGGGRGVGGGDYLLQRLVDVA
                     RHADRTGDRVVRQAIADLYIRLTIAGYFQGRL"
     assembly_gap    1409..1449
                     /estimated_length=41
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1450..1666
                     /locus_tag="PLV68_06160"
     CDS             <1450..1666
                     /locus_tag="PLV68_06160"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HRE00863.1"
                     /translation="VLTDNLQRSSRLASMLLGPRLIADAGDWGTYAWSELILGTPGLR
                     VGGGTDEVQKNMLGERVLGLPREPRPN"
     gene            1838..>3043
                     /locus_tag="PLV68_06165"
     CDS             1838..>3043
                     /locus_tag="PLV68_06165"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRE00864.1"
                     /translation="MALAIAACGSDDNKTTSSSTTVSVPASADDGTTTTSTAEPPGPS
                     GSGTPNAPGREEAIAAANATVKDDPLSGAAATGLTRGVSDSTIKIGCIFEVANYAGFE
                     DAIAARFKSGDVNGRTLEILPCEDDAGDPANTLSIAKRLVEQSQVFGIITATQGMSPA
                     VATYLNDNQVPYIGWGFVDSFCAKRWAFGFDGCLNGLFSDPKDVPHAYVNPYLVQPVV
                     EASGIPPADVRAAIIGSDNDAGRGGNANYSSLFVNLGATVVYAEAVVPVPGPTTDFTP
                     FVNAALEKDPNIILISTQFSDVGGLTAALKAAGFDGSIMNFVAYVPGLLDALPQLSEA
                     LDGTYVLASLVPQEQQNGYIKVVEDALEASGAETGRFITFGGALGFLMGDQMASMINA
                     AGPDLNTTTF"
     assembly_gap    3044..3080
                     /estimated_length=37
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3290..3312
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3341..>4645
                     /locus_tag="PLV68_06170"
     CDS             3341..>4645
                     /locus_tag="PLV68_06170"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00865.1"
                     /translation="MDKFLTLAVSGLITGAIYSLVAAGVTLSHAATGIFNFAYGAIAY
                     VCAVTYFELNTARQWSIVPAALVTLCIVAPAIGVLLDWAVFRRLTNASPAAKVVAPVG
                     LMIALPAATRYIIDALVNWFGVALVKSTQLNSVGFPSGVGPVPRKQWRLPGGANLDSN
                     QLALLIVAVVSAVALWALMRKSYLGLRMRAVVDSGSLASLRGIDTSRTSRTAWIIGCV
                     LAGLAGVFGAPVLGSLAPNSFVAVQFVACAAAVLGGLRNVPLAALGGVLLGVTQNLVF
                     GYATFARDIKGFNTAVPFVILLGGLIVVTRDRRRSGGTVADEGDAASLGRIPWFTATV
                     SGLFLLWLFLLADNYWVGVTILGLCIAIVLLSFVVVTGLGGMVSLAQATFVTAAGLMT
                     GLLLDGHGWPFIPAAIVGILFSAAIGVLVALPALRLGGLPFAL"
     assembly_gap    4646..4655
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4656..>5382
                     /locus_tag="PLV68_06175"
     CDS             <4656..>5382
                     /locus_tag="PLV68_06175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00866.1"
                     /translation="ATLALAFLGDSVLFQWDPLRNKQTGWTFSPPKIGPIDLADRYTF
                     AWVLVALIVLCIAIVRNIERSATGRAIMAVRGSALAASTSGHEPARIAGLGGVMLATA
                     NRSMVNSSYPATVGLLWLAQVVLFGVRRPGTAVAAGISAALFPELLRTGFHWPSWVPS
                     FLSWDGTRSVEIASILFGLGAVAVAQNPDGFLSVYAAMFRSRRALRIRALQTAEEIEH
                     EPSLAAVASAQPAVPSAVENPALE"
     assembly_gap    5383..5485
                     /estimated_length=103
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          820 a         1825 c         1829 g         1041 t
ORIGIN      
        1 gccggccatg tcgaactctt cgcggcgata gccggcgccg gccacgaagc tgaaccggcc
       61 cggcccgatc aggtcgatcg tcgccaactg ctcggcgagg cggaccggat catggagggg
      121 gaccagcgcg gcagcgacat tgatcttgat ccgcttggtc ctgcccagga tcgcggctgc
      181 gagcgtgaag ggcgcgctga ggtatccgtc gggcgtcccg tggtgttcgg agagcgcgac
      241 gaagcggaat ccgagcccct cggcccactc cgccatgtcg agcgcggcgg catattgcgt
      301 gcggtgatcc accgttgcga acgacggcac gcgcaggtcg aagcgaatac cgagacggat
      361 cggactcgtg actctgccag aggttgttga ggttctcacg cgagcagtct gctacagacc
      421 tctcatccca atcaaccgtt gggtttgtgt actccgccat cgtaggatgc atctgtgacg
      481 gtcgatgagg gcacctttcg cgacgaggtg ttggccttct tgcggaacca cgctgctgaa
      541 cggcgaacgg cccacaccgg ttggggtgag gggtccgacg tggtcggctt gttcgaagac
      601 gagcaggagg agaagcggct gctcgcactg gctnnnnnnn nnnnnnnnnn nnnnnnnggg
      661 cttgggatgg atcgctggcc ctccggagta cggcggtcgc gggctgccca agcagttctc
      721 ccgtctctac aacgagctcg aagagggttt cgccacggcc gaccagatgc gcctctccgt
      781 tggcctcggc atggtcgggc cgacgatcgc tgcccacgga tcggcggagg tcaagaaccg
      841 ctatctgcgc tcgatctacc gcggtgacgt cctggcctgc caacttttct ccgagccgaa
      901 cgccggctcc gatttgtccg ccatccgcac ccgcgccgaa cgcgacggcg actcgtggcg
      961 catcaccggg cagaaggtgt ggacatcggg cgggcacttc agcgacatcg gcatggtgct
     1021 ggcccggacc ggtaccgacg actcgcgcac gcgcggtctg acgatgttct tgatcgacat
     1081 gcacgccctt ggcgtcgagg tccgccccct ccggcagatg accggccacg cctccttcga
     1141 cgaggtgttc ctcgacgacg tcctcacgcc tgattcgaac cggctcggcc aggtcgacga
     1201 cggctggtcg gtgatgatga ccaccctgct caatgagcgt ggctcgatcg gtgggggagg
     1261 acgtggcgtc ggcggcggcg actacctcct ccagcgcttg gtggacgtgg cgcgtcatgc
     1321 cgatcgcacg ggtgaccggg tcgtccgcca ggcgatcgct gatctgtaca tccggctcac
     1381 tatcgccgga tacttccagg ggcggctcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnc gtgctcaccg acaacctgca gcgctccagc cgtttggcca gcatgctgct
     1501 gggtccgcgt ttgatcgccg atgcggggga ctggggcacc tacgcctggt ccgaactgat
     1561 cttgggcaca cctggtctcc gcgtcggcgg tggaacggac gaggtgcaga agaacatgct
     1621 cggcgagcgt gtgctgggct tgcctcgcga gccgcgcccc aactgaacca accggtcggc
     1681 agaagatcgc ttgacgtgct gcggccactc atctagattt cgctcatacc agacggttgt
     1741 ttggtgattg tgaacctgag ggggaatcga gatgctaggc actggcgcag cgccgcacaa
     1801 cccgcgcagg ctgcgcggac tcgtggcggt ttcggcgatg gcactggcca tcgctgcatg
     1861 cggaagcgac gacaacaaga cgacttcgtc gtcgacgacc gtctcggtgc cggcctcggc
     1921 cgacgacggg acgacgacta cctcgactgc ggaacccccc ggccccagtg gctccggcac
     1981 gccgaacgcc ccgggtcgcg aggaagcgat cgcggctgcg aatgcgacgg tcaaggacga
     2041 cccgctgtcg ggcgcggcgg ccacgggact cacccgcgga gtcagcgaca gcacgatcaa
     2101 gatcggctgc atcttcgagg tggccaacta cgcaggcttc gaagatgcca tcgccgctcg
     2161 cttcaagtcg ggcgatgtca acggtcgcac gctggagatc ctgccctgcg aggatgacgc
     2221 tggcgatccg gccaacacct tgtcgatcgc caagcgactg gtcgagcaga gccaggtgtt
     2281 cggcatcatc accgccaccc agggtatgtc gcctgcggtc gccacctacc tgaacgacaa
     2341 tcaggtcccc tacatcggct ggggttttgt cgactccttc tgtgccaagc gctgggcgtt
     2401 cggtttcgac ggctgcttga acggcctgtt ctccgatccg aaggatgtgc cgcacgccta
     2461 cgtgaatccg tatctggtgc aacccgtggt cgaagcctct ggcatcccgc ctgcggacgt
     2521 acgcgccgcc atcatcggca gtgacaacga cgctggacgc ggtggcaacg ccaactactc
     2581 gtcgctgttc gtcaacctcg gggccaccgt ggtgtacgcc gaggccgtgg tacccgtgcc
     2641 aggcccgacc acggacttca cgccgttcgt caacgcggcg ctggagaagg atcccaacat
     2701 catcctgatc tccacccagt tcagcgacgt cggcggtttg accgcggcgc tgaaggcagc
     2761 cggcttcgac gggtcgatca tgaacttcgt cgcctacgtg ccgggcttgc tcgacgcgct
     2821 gccgcagctg tcagaggcgc tcgacggcac ctacgtcctc gcctcgctcg tgccgcaaga
     2881 gcagcagaac ggctacatca aggtcgtgga ggatgcgctc gaggcgtccg gtgccgagac
     2941 cggccggttc atcaccttcg gtggtgcgct cggcttcctc atgggcgacc agatggcttc
     3001 gatgatcaat gctgccggcc cggacctcaa cacgaccacc ttcnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn acacctatac ctcgcccggt gaaggcggcc cctgctcgat
     3121 cagcttcccc ggtgcgcact actactcggc gagcggttcg gcgctggtca aggtcgtcgg
     3181 tggcaagttc gaggtcacct cgccctacac ctgctacccg ccggtggaac aaactcgagg
     3241 ctgatcgctt ccggatcggt caagcaacga cgtcgaagac ccgaggtggn nnnnnnnnnn
     3301 nnnnnnnnnn nncccggccc acctgctgtg aggagatgcc ttggacaagt tcctgacgct
     3361 cgctgtgagc ggactcatca ccggtgcgat ttattcgctc gtagcggcag gggtcaccct
     3421 gtcgcacgcg gcgaccggga tcttcaactt cgcctacggc gcgatcgcgt acgtctgcgc
     3481 ggtcacctac ttcgagctca acaccgcgcg gcagtggtcg atcgtgcctg ccgcgttggt
     3541 gacgctgtgc atcgtcgctc cggcgatcgg tgttctcctc gattgggctg tgttcagacg
     3601 gctgaccaac gcctcgccag cggccaaggt cgtcgccccc gtcggtctga tgatcgcgtt
     3661 gccggccgct acgcgctaca tcatcgatgc cctcgtcaac tggttcggcg tcgccttggt
     3721 gaagagcacg cagctgaact cggtcggctt cccgtccggt gtcggccccg tgccgcgcaa
     3781 gcagtggcgc ctgcctggcg gggccaacct cgactccaat caactcgccc tgctgatcgt
     3841 cgcggtcgtc tcggctgtgg cgctgtgggc gctgatgcgc aagtcgtatc tcggtttgcg
     3901 catgcgcgcc gtcgtcgaca gcggctcgct ggcgagcctt cgaggcatcg acacctcgcg
     3961 gacgtcgcgc accgcctgga tcatcggctg cgtgctcgcc ggcctcgccg gcgtgttcgg
     4021 agccccggtg ctcggcagcc tggccccgaa ctcgttcgtc gcggtccagt tcgtcgcatg
     4081 cgccgcggca gtgctcggtg gcctgcgcaa cgtgccgctc gcggcgctgg gtggggtgct
     4141 gctcggtgtg acccagaacc tcgtcttcgg gtacgccacc ttcgctcgcg acatcaaggg
     4201 cttcaacacc gcggtgccgt tcgtcatcct gctcggcggc ttgatcgtcg tgacccgtga
     4261 tcgccgtcgc tcaggtggga cggttgccga cgaaggtgat gctgcgtcgt tggggcgtat
     4321 cccgtggttc accgcgacgg tttccggtct tttcctgctg tggctgttcc tcctcgccga
     4381 caactactgg gtcggcgtga ccatcctcgg gttgtgcatc gcgatcgtgt tgctttcctt
     4441 cgtcgtcgtg acggggctcg gaggaatggt gagcctggcc caggcgacgt tcgtcacagc
     4501 cgccgggttg atgaccgggc tgctgctcga cggccacggt tggccgttca taccggcggc
     4561 gatcgtcggc atcctctttt cggcggcgat cggtgtgctg gtggcattgc cggccttgcg
     4621 tctcggtgga ttgcccttcg cgctcnnnnn nnnnngccac cctggcgctc gcgttcctcg
     4681 gtgacagcgt gttgttccag tgggacccgc tgcgcaacaa gcagacggga tggaccttca
     4741 gcccgcccaa gatcgggccg atcgatctgg ccgatcggta tacgttcgcc tgggtgctgg
     4801 tggcgttgat cgtgctgtgc atcgcgatcg tgcgcaacat cgagcggtcg gcgaccggcc
     4861 gcgcgatcat ggccgtgcgc ggctcggcgt tggctgcgtc gacctcgggc catgagccgg
     4921 cgcgcatcgc gggtctcggc ggcgtgatgc tcgcgacggc caaccggagc atggtcaaca
     4981 gctcgtaccc cgccaccgtg gggttgctct ggctggcgca ggtggtcttg ttcggcgtgc
     5041 gtcgtcctgg caccgcggtc gcggcgggca tctcggcagc gctgtttccg gagttgctcc
     5101 gcaccgggtt ccattggccg tcatgggtgc cctcgttcct gtcgtgggat ggcacgcgct
     5161 cggtcgagat cgccagcatc ttgttcggct tgggtgccgt tgcggtcgcc cagaaccccg
     5221 acggcttcct ctcggtgtac gcggcgatgt tccgctcccg gcgggcactg cgaatcaggg
     5281 cgttgcagac ggcggaggag atcgagcacg agccgtcgct cgccgctgtc gcgagcgcgc
     5341 agccggccgt gccgagcgcg gtcgagaacc cagccctcga acnnnnnnnn nnnnnnnnnn
     5401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5461 nnnnnnnnnn nnnnnnnnnn nnnnncaaga cgacgctgcc acgagctgtg tctggactca
     5521 tcccgacctc gggtggcgtt ctcgagttgg atcaggtcgt actgacatcg atgccgatcc
     5581 acaagcgcag tcgcggtgac cggctcgtcc tcgtgcccca agatcgtggc gtgttcccag
     5641 gactgacggt ggacgagaac ctccgtgtct ggctccgtga tccggccgac cgggccaagg
     5701 cgtacgcgcg ctttcccgca ctcggacaac gccagcacac gcatgcgggc agc
//
LOCUS       DAORNF010000118         5706 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_10442_length_5706_cov_1.709078, whole genome shotgun sequence.
ACCESSION   DAORNF010000118 DAORNF010000000
VERSION     DAORNF010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 5706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5706
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10442_length_5706_cov_1.709078"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>991)
                     /locus_tag="PLV68_06180"
     CDS             complement(<1..>991)
                     /locus_tag="PLV68_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00867.1"
                     /translation="WVHRANVGQHHLYDVDVEYPLGHDPLPAGAQARFDVSLEVPREF
                     AAGRHALAVEVSSDRRDERPVLTGVTVDVSSIQKVALRINPGNIRGHRTGRFRVELDN
                     LETHPVDLSLEGHGQDVEVGFGRKRVQMQPGDSVSVSGTVRGNRGAVRDSVKHLITVS
                     ATGRSQPIYAEASFEQRPLIARRFRSAMAVLALLGLWAGILGGAAYWYAQRDDKPDDS
                     TASGQLVDTDGDGIPDAPLGSLPTGTGADGSGTGGQPGSSGGNGGTSGSGQGAPGAPV
                     NKRPTSTVISGTVKAGETGNNSGVTVTLVPATLGGTDTGNDPARAAALSRGGTG"
     assembly_gap    992..1046
                     /estimated_length=55
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1133..>2504)
                     /locus_tag="PLV68_06185"
     CDS             complement(1133..>2504)
                     /locus_tag="PLV68_06185"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRE00868.1"
                     /translation="VEADDLTGITAVLVVPGTSSDDITAEVLRVAVSADGTFLFENVP
                     SPARYQLVVSKPGYASEIRNVSVGAAQKVENIEVMLRKGDGVIRGRVNGPGGPLGGAT
                     VEVTDGLSAVSTVSLTDGDVGAFALRSLATPATYTITVSRPGYTSETRTVNLAAAQDL
                     DAGTVVLQRATGSLSGTVSQTGIGAIGGVTVTITAGDTRVVTSTASVGNVGTFYVDQL
                     PIPATYNITFSRAGLDTQVRVQDLDPNRVADATGIDVAMPKSSATVAGVVRNVAGDPV
                     ANATVTLTDGSNSRTLITAHEPLGRFSFSGVSPGSYTLTANLPGSTQAVQLVTVLPNV
                     DKDLDISLSSQASVVGRVMVLSSALPTTSDPDSTTAPPTYVPYAGATVRIFAAGAFPG
                     GVAIDSIITNADGYYAFTALEAPQNYVIAVYQSGNAPDPLDSIMILTQPSTELTVTTF
                     EIPVIF"
     assembly_gap    2505..2604
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2605..3614)
                     /locus_tag="PLV68_06190"
     CDS             complement(<2605..3614)
                     /locus_tag="PLV68_06190"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HRE00869.1"
                     /translation="MYADVTPNVAEIGPGESCTVTVRVSNSSPLIDAYSVQVFGVDPQ
                     WVTVSPPRLSLFPDESGTVEVTIQLPVSFPAGHRQLSVNVSSENDPEQFTLSSLGLMA
                     LGHHRLRMRLDPITVTGASEAVFGLIVTNDGNTTVTAVASAMDQEELTGTIISPEVIE
                     LLPGQQETLEATVSGPRPWFGQPKVRVINFAVESTSRIETIGTFVQRPRISRWMLSLM
                     GLRAAAAVFAAILSRTFSTVVDEAAIDKNLINEALDKGGGGGVMVPVDPGSVTGKVVL
                     FSTGDGVGGVQAELFKAGDTRVPVATSATNDDGSYVFSRLGTGTFKVRFSGAGFNAVW
                     YES"
     gene            complement(3630..4562)
                     /locus_tag="PLV68_06195"
     CDS             complement(3630..4562)
                     /locus_tag="PLV68_06195"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRE00870.1"
                     /translation="MVTPASYALRIEATGFDPREIIETLGAGENKVINTITLGAADGG
                     FRGTVLGPNGTGLGNVEVAVRSGDIVKTTKTPTSGNVGSFIVDSLETPRTYTLTFSLA
                     GYADQTIAVDLQSGETRSLTVTLTGGQATVSGQVTDEGGVPIGGAIVNVGCGTFTANT
                     ATLTTSGAADQAGTYSVVGVPAPATCTIVVASDGYLSQTIRIDVATAGFLGGNDVILP
                     RATGSVSGTAWLGVGTAAAGATDVIVELSNGTTNAVRSTVTTSSPAGLYRFADIVPGD
                     YTLTFRKAGLVTRIVIVTVTAGQTVQRDVNLSAS"
     gene            complement(4556..>5706)
                     /locus_tag="PLV68_06200"
     CDS             complement(4556..>5706)
                     /locus_tag="PLV68_06200"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRE00871.1"
                     /translation="GARQSGAPLLRFADLERGMSLRIDLPTNCLAGEYLVSVQVVSIV
                     DPQRTSEHEFWLTVEPLLAATITMHPSLIVGGSHAVFQASIANVGNVTADVAVNARDA
                     TRHFSCLAYPATVSVPAGETHTVEVHTDGKRPWFGDPIARPIDVEAVAASPTPTVNGD
                     ITLPATVTFTQKPRIPRGALTIAILAGIIALWAAVFLLVINALRDTSAPTKLTAVDFA
                     QGGSSEIDLTAMASSLAGTVTSESTREPLPRITVEAYRVKFDASQELTGSAGTDDDGA
                     FALESMLPGTYTLRLTADGFEEVWYPASPNQAGAEKVRITKDAPGEGLNVVMVGKPGS
                     MSGQIVAPESQGSATSISLTITQQVTNPVEGAPLPEPVPVPRSLSPRW"
BASE COUNT          814 a         1764 c         2057 g          916 t
ORIGIN      
        1 gccggtgccg ccgcgtgaca gcgccgccgc ccgtgccgga tcgttgccgg tgtcggtgcc
       61 gccgagggtg gccggcacca gggtgacggt cacgccgctg ttgttgccgg tctcgcctgc
      121 tttgacggtg ccgctgatga ccgttgacgt gggccgcttg ttgaccggcg cgcccggggc
      181 gccctggccc gaaccgctgg tgccaccgtt gcctccgctg ctcccgggtt ggccaccggt
      241 gcccgacccg tcggcgccgg tgccggtggg cagtgacccg agcggggcgt cggggatgcc
      301 gtcaccgtcg gtgtcgacca actgcccgct cgcggtggag tcgtcgggct tgtcgtcacg
      361 ctgggcatac cagtacgcgg cgccgccgag gattccggcc cacaggccga gcagggccag
      421 cactgccatg gccgagcgga agcgacgggc aatcagtgga cgctgctcga acgacgcctc
      481 ggcgtagatc ggctgcgagc gccctgtggc cgacacggtg atgaggtgct tgaccgaatc
      541 gcgcacggcg ccccggttgc cgcgaacggt gccggacacc gacaccgaat cgcccggctg
      601 catctgcacc cgcttgcgcc cgaaaccgac ctcgacgtct tggccgtggc cttcgaggct
      661 gaggtcgacc gggtgcgttt cgaggttgtc gagctcgacc cggaaccgtc cggtgcggtg
      721 gccgcggata ttgccggggt tgatccgcag ggcgaccttt tggatcgacg acacgtcgac
      781 ggtgacgccg gtgagcaccg gacgttcgtc gcgacggtcg gagctgacct cgaccgcgag
      841 cgcatggcgc ccggcggcga actcgcgcgg cacctccaac gagacgtcga agcgggcttg
      901 cgcaccggcg ggcagcgggt cgtggccgag cgggtactcg acgtcgacgt cgtacaggtg
      961 gtgctgaccg acgttggctc gatgcaccca gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1021 nnnnnnnnnn nnnnnnnnnn nnnnnncggg gtcgagcaat tcgatcttca tgaagttcgc
     1081 ccgatctggt gatggcgccg gtctgccctc cgacgtccta gcccacgatg catcagaaga
     1141 ttaccgggat ttcgaaggtg gtcacggtca gttcggtgct gggttgcgtc aagatcatga
     1201 tcgagtcgag cgggtcaggg gcgttgcccg actggtagac ggcgatgacg taattctggg
     1261 gcgcttccag cgcagtgaac gcgtagtacc cgtcggcatt ggtgatgatc gagtcgatcg
     1321 cgacgccgcc ggggaacgct ccggcggcga agatgcgcac cgtggctccg gcgtagggca
     1381 cgtaggtggg tggcgcggtg gtgctgtcgg ggtcgctggt cgtcgggagt gccgacgaca
     1441 gcaccatcac ccggccgacc acagaggctt gtgacgacag cgagatgtcg aggtccttgt
     1501 cgacgttggg cagcacggtg accaactgga cggcttgggt gctgcccggc aggttggcgg
     1561 tgagggtgta cgaccccggg ctgacgccgc tgaagctgaa ccggccgagc ggttcgtggg
     1621 cggtgatcag cgtgcgcgaa ttgctgccgt cggtgagggt gaccgtcgcg ttggcgaccg
     1681 ggtcaccggc cacgttgcgc accacgccgg cgacggttgc gctggacttc ggcatcgcga
     1741 cgtcgatgcc ggtggcatcg gcgacgcggt tggggtcgag gtcctgtacc cgaacctggg
     1801 tgtcgagccc cgcacggctg aaggtgatgt tgtaggtggc ggggatcggt agctggtcga
     1861 cgtagaacgt tccgacgttg ccgacgcttg cggtggacgt gacgacccgg gtgtcgcccg
     1921 cggtgatcgt gacggtgaca ccgccgatcg ctccgatgcc ggtctggctg accgtgccgc
     1981 tgagcgaccc ggtggcgcgt tgcagcacca ccgtgcccgc gtcgaggtct tgggccgcgg
     2041 ccaggttgac cgtgcgcgtc tcgctggtgt agccgggccg tgagacggtg atcgtgtacg
     2101 tggcgggcgt ggccagcgag cggagtgcga atgcgccgac gtcgccgtcg gtgaggctca
     2161 ccgtcgacac ggccgacaac ccgtcggtga cctcgaccgt ggcaccgccc aggggcccac
     2221 cggggccgtt gacgcggcca cggatgacgc catcgccctt gcgcagcatg acctcgatgt
     2281 tctccacctt ctgcgccgcg ccgacggaca cgttgcggat ctcactggcg tagcccggct
     2341 tggagaccac gagttggtag cgcgccggcg acgggacgtt ctcgaacagg aaggtgccgt
     2401 cggccgacac ggcgacgcgc aacacctcgg cggtgatgtc gtcggacgag gtgcccggca
     2461 cgaccaacac cgcggtgata ccggtcaggt cgtcggcttc gaccnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnngattcg taccacaccg cgttgaaacc tgcgccgctg
     2641 aagcgcacct tgaacgtccc ggtgccaaga cggctgaaca cgtagctgcc gtcgtcgttg
     2701 gtggccgagg tggcgaccgg cactcgcgtg tcgccggcct tgaacagctc tgcctgcacg
     2761 ccgccgacgc catcgccggt gctgaacagc acgaccttgc cggtgaccga gccggggtcg
     2821 accggcacca tcaccccacc gccgccgccc ttgtcgagtg cctcgttgat gaggttcttg
     2881 tcgatggcgg cctcgtcgac cacggtgctg aaggtgcggc tgaggatggc cgcgaacacc
     2941 gcggctgcgg cccgcagacc catcagcgac agcatccagc ggctgatccg ggggcgttgg
     3001 acgaaagtgc cgatcgtctc gatgcggctg gtgctctcga ccgcgaagtt gatgacccgc
     3061 accttgggct ggccgaacca gggtcgcggt cccgacacgg tggcttccag ggtctcttgc
     3121 tggccgggca gcaactcgat cacctcgggg gagatgatcg tgccggtgag ctcttcctga
     3181 tccatcgccg atgcgacagc ggtgaccgtg gtgttgccgt cgttggtgac gatcagcccg
     3241 aacacggctt cgctggcgcc ggtgaccgtg atcgggtcga ggcgcatgcg caagcggtgg
     3301 tggcccagcg ccatcagacc cagcgagctg agcgtgaact gctcggggtc gttctccgag
     3361 ctgacgttga ccgacagctg gcggtgaccc gcagggaacg acaccggaag ctggatcgtg
     3421 acctcgacgg tgcccgattc gtccgggaac agcgacagcc gcggcgggct gaccgtgacc
     3481 cactgcgggt cgacgccgaa cacctgcacc gagtaggcgt cgatcaacgg tgacgagttc
     3541 gagacgcgca cggtgaccgt gcagctctca cccggcccga tctcggcgac attgggagtg
     3601 acgtcggcgt acatctcagt gcactcccgt caactcgcgc tcaggttgac gtcgcgctgc
     3661 acggtctggc cggcggtgac ggtgacaatg acgatgcggg tgaccagccc ggccttgcgg
     3721 aaggtgagcg tgtagtcgcc cgggacgatg tcggcgaagc gatacagccc agccggcgac
     3781 gaggtggtca cggtggaacg cacggcattg gtggtgccgt tggagagctc gacgatgacg
     3841 tcggttgcgc cggccgctgc tgtcccgacg ccgagccacg cggtacccga caccgacccg
     3901 gtcgcacgcg gcaggatgac gtcgttgccg ccgaggaaac cagcggtggc gacgtcgatg
     3961 cggatggtct ggctgaggta gccgtcgctg gccaccacga tggtgcaggt cgccggggcg
     4021 ggcacgccga cgacggagta ggtgccggcc tggtcagcgg cgcccgacgt ggtcagcgtt
     4081 gcggtgttgg cggtgaaggt accgcagccg acgttgacga tcgcgccccc gatcggcacg
     4141 ccgccctcgt cggtgacctg gcccgacacc gtggcttgcc caccggtgag tgtcaccgtc
     4201 agcgatcggg tctcacccga ctggagatcg acggcgatgg tctggtcggc gtagcccgcg
     4261 aggctgaagg tgagtgtgta ggtcctcggc gtctccaacg aatcgacgat gaacgatccg
     4321 acgttgcccg acgtcggggt cttggtggtc ttgacgatgt cgccgctgcg caccgcgacc
     4381 tcgacgttgc ccagcccggt gccgttgggg ccgagcaccg tgccgcggaa gccgccgtca
     4441 gccgcgccga gggtgatggt gttgatcacc ttgttctcgc ccgcgcccag agtctcgatg
     4501 atctcgcgcg ggtcgaagcc ggttgcttcg atgcgcagcg cgtagctggc cggcgtcacc
     4561 atctggggga gagagaacga ggcaccggca ccggctccgg cagcggagcg ccctcgaccg
     4621 ggttggtcac ctgttgcgtg atcgtcaacg agatcgacgt ggccgagccc tggctctcgg
     4681 gcgcgacgat ctggccgctc atgcttccgg gcttgccgac catgaccacg ttcaagccct
     4741 cgccaggcgc gtccttggtg atccgtacct tctcggcacc ggcctggttg ggcgacgcgg
     4801 ggtaccacac ctcctcgaag ccgtcggcgg tgaggcgcag ggtataggtg ccgggcagca
     4861 tcgattcgag ggcgaaggcc ccgtcgtcgt cggtgcccgc cgaaccggtg agctcctggc
     4921 tggcgtcgaa cttgactcga taggcctcga cggtgatgcg gggcaacggc tcgcgggtcg
     4981 actcggacgt gacggtgccg gccaatgacg acgccatggc ggtgaggtcg atctcgctgc
     5041 tgccgccttg ggcgaagtcg accgcggtga gcttggtggg tgccgaggtg tcgcgcagcg
     5101 cgttgatcac caacaggaac accgctgccc acaacgcgat gatgccggcc aggattgcga
     5161 tggtcagcgc accgcggggg atgcgcggct tctgggtgaa cgtcaccgtc gcgggcagcg
     5221 tgatgtcgcc gttgacggtg ggcgtcgggc tggcggcgac ggcctcgacg tcgatcgggc
     5281 gggcgatcgg gtcgccgaac cacgggcgct tgccgtcggt gtggacctcg acggtgtgcg
     5341 tctcgcccgc gggcaccgac acggtggccg ggtaggcgag gcagctgaag tggcgggtgg
     5401 cgtcgcgggc gttgacggcg acgtcggcgg tgacgttgcc gacgttggcg atcgaggcct
     5461 ggaacacggc atggctgccg cccacgatca ggctggggtg catggtgatc gtggccgcga
     5521 gcagcggttc gacggtgagc cagaactcgt gttcggacgt gcgctgcgga tcgacgatcg
     5581 agacgacctg cacgctcacc aggtactcgc cggcgaggca gttggtgggc aggtcgatgc
     5641 gcagcgacat gccgcgctcg aggtcggcga agcgcaggag cggcgcgccg gactggcgcg
     5701 ccccca
//
LOCUS       DAORNF010000119         5703 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_10449_length_5703_cov_1.843130, whole genome shotgun sequence.
ACCESSION   DAORNF010000119 DAORNF010000000
VERSION     DAORNF010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 5703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5703
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10449_length_5703_cov_1.843130"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            110..>328
                     /locus_tag="PLV68_06205"
     CDS             110..>328
                     /locus_tag="PLV68_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HRE00872.1"
                     /translation="MSHLSLFLIDLGAISLLAFAIYFPRHRRRDMLLAYVGLNVGVLA
                     VTTALANSAVTAGLGLGLFGVLSIIRLRS"
     assembly_gap    329..363
                     /estimated_length=35
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <364..733
                     /locus_tag="PLV68_06210"
     CDS             <364..733
                     /locus_tag="PLV68_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HRE00873.1"
                     /translation="AMGLLCGVSITPAWLTPALLGAMLVAVFVIDSPRLFGRYRHTSV
                     TLDAAYPDERELVARLESMLGGSVKRYAVTKLDLVHDLTVVDVRYQLGADRAPGTTPP
                     ASVEARDRADSPSWLSEQIG"
     gene            736..1503
                     /locus_tag="PLV68_06215"
     CDS             736..1503
                     /locus_tag="PLV68_06215"
                     /inference="COORDINATES: protein motif:HMM:NF020916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HRE00874.1"
                     /translation="MASPSATLTAIGADMPATSLDEVLAAAELTTRVDRKYIVPHAMI
                     HEVVACVTGAASSDLHVLEIDGRRSSLYETVYFDTPNRQSYLGAAYGRRRRFKVRTRT
                     YLDSDRCQLEVKLNGGRGESAKVALAMPSRDRGRITAEGYAFISEHLPVTLDGPLHPA
                     VTTRYRRMTVVDYAGTARITCDTDLSMEAPDGRVACLDAAVVVETKSSGPPTMFDRVL
                     WRHGCRPAPISKFAVGMALLVPGLPANKWHRTLNRPS"
     gene            1622..>2495
                     /locus_tag="PLV68_06220"
     CDS             1622..>2495
                     /locus_tag="PLV68_06220"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRE00875.1"
                     /translation="MDAAPRVALALGSGGARGYAHIGVIQVLREQGYEIAGVAGSSMG
                     ALVGGLYAVGRLDDFTDWALGLTRFDVLRLMDVSFTAPGAIRAEKILNRVRDLAEGVL
                     IEDLPIPYTAVASDLGARKAVWFQRGPLDVAIRASIAIPGVFAPVMLNGRILVDGGAM
                     DPVPMLALAAIRADFTIAVDLGGLRSPVTDEPLEVSGESRPMEEWADKLKRTASNLFD
                     RDLVRSLMSRLDRGRDSADDTINDSASDSINDSAGDSGDKLGSEIEDVDVGITDKIAD
                     LRVHERDHEPLAVFE"
     assembly_gap    2496..2688
                     /estimated_length=193
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3006..3839
                     /locus_tag="PLV68_06225"
     CDS             3006..3839
                     /locus_tag="PLV68_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00876.1"
                     /translation="MESSRSCWCAKQDADDSKAALSAVNESLSDLTDQADSDADSVKD
                     QIDALLDQQATDKTTQQQLQATIADLNEQITTLTNQVANGATGGGEDVAALQTQLEQA
                     RQDLADVQAQLTETQDDLDEALANANTTAAPFDVEAGKVPLGGGNFSVVTNRVTCDGY
                     ADAASSCPDTFTVDGRIFSEGDGYVIEFANIARVSMGSFDGFNYGGQAPATGDVSPTC
                     NGNPVATTFAVQIAPVRYHVDPATQAVSSTAFSFTWTMSAPAGGGCAATTRVYTGTIA
                     L"
     gene            3935..4657
                     /locus_tag="PLV68_06230"
     CDS             3935..4657
                     /locus_tag="PLV68_06230"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HRE00877.1"
                     /translation="MLIAGLSDFVTGDLTDWVVRVIEAISYLGVALLVALESVFPPIP
                     SEVVLPAAGLTAFRGDANLIGMMVAATVGSVVGAWVLYLIAAAIGHRRLHALVVRYGK
                     WLGVKEKDLLRAESWFDDHANTAVLICRCIPLIRSLVSIPAGFRRMNALQFTIYTTVG
                     SAVWNVVLIGAGYRLGDHWERVGDWVGVLQYVVVAAILVVLAIWVWRRFISPTHKARR
                     RAEELQEAMQIQVAEAILDATD"
     gene            4796..>5433
                     /locus_tag="PLV68_06235"
     CDS             4796..>5433
                     /locus_tag="PLV68_06235"
                     /inference="COORDINATES: protein motif:HMM:NF026010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00878.1"
                     /translation="MKRKIWRALTLRCPWCGSRKTFILQYRIGRRSRTMSLGYTRVIT
                     PDNARGMAIAHLAAIENNRDPLADRDDHRDALSFRELAMRFDAEHIAVHIKPSTGAEY
                     RRALKKAILPFLGRRPVAEVSREEIARFRHKYRDVPYFANRCLAILSKMFNLAELWGL
                     RPDGSNPCRHIRKYKEERRERFLSAAELRRVGQVLAEMVAERVEMPSALTAIR"
     assembly_gap    5434..5488
                     /estimated_length=55
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5489..>5703
                     /locus_tag="PLV68_06240"
     CDS             <5489..>5703
                     /locus_tag="PLV68_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00879.1"
                     /translation="GALNLPDSKTGAKVVYLGQPALDVLRSAPRLPGNPWVIWGKKHG
                     THLYGLQAVWERVRARAGLKDVRIHDL"
BASE COUNT          871 a         1844 c         1789 g          916 t
ORIGIN      
        1 tcgtcgggaa ctatcgtgac gggtcataac gtctgaccag gaattgttca accccgattc
       61 accgtggctt cacagcgtgt tctcagcttg agcctccagg atgtcgtcca tgtcgcacct
      121 ctcgttgttc ctgatcgacc tgggcgccat cagcctgctc gcgtttgcga tctacttccc
      181 gcgtcatcgg cgccgcgaca tgctgctcgc gtacgtcggg ctcaacgtcg gtgtgttggc
      241 ggtgacgaca gcactcgcca actccgccgt caccgccggg ttgggcctcg gcctgttcgg
      301 tgtgctgtcg atcatccgcc ttcgctcgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnggcgatg ggcctgctgt gcggcgtgtc gatcacaccg gcatggctca ccccggcgct
      421 gctcggagcg atgctcgtgg cggtgttcgt catcgattca ccgcgactgt tcggccgcta
      481 ccgccacact tccgtcaccc tcgacgctgc gtacccggac gagcgtgagt tggtggcccg
      541 cctggagtcg atgctcggcg gttcggtcaa gcgctacgcc gtgaccaagc tcgacttggt
      601 gcacgatctg accgtggtcg acgtgcgcta ccaactgggt gcggaccgcg ctcccggcac
      661 aacgccgcct gcgtcggtcg aggctcggga tcgcgccgat tcgccgagct ggctcagcga
      721 gcagatcggg tgatcatggc cagcccgtct gcaaccctga ccgcgatcgg cgcggacatg
      781 ccggcgacat cgctggacga ggtgctggcc gccgccgaac tgacgacccg agtcgaccgc
      841 aagtacatcg ttccgcatgc aatgatccac gaggtcgtcg cctgtgtgac cggagcggca
      901 agcagcgacc tgcacgtgtt ggagatcgac ggccgacgaa gctcgctgta cgagacggtc
      961 tatttcgaca cgcccaaccg ccagagctac ctcggtgcgg cctacggccg tcggcgccgg
     1021 ttcaaggtgc gcacccgcac ctacctcgac tccgaccgct gccagttgga ggtcaagctc
     1081 aacggcggtc gcggcgagtc ggccaaggtc gcgctggcga tgccatcgcg cgatcgaggc
     1141 cgcatcactg ccgaagggta tgccttcatc agcgaacacc tgccggtcac cctcgacggc
     1201 ccactgcatc cggcggtcac gacgcggtat cggcggatga cagtggtcga ctatgccggc
     1261 accgcgcgca tcacctgcga taccgacctg tcgatggagg cacctgacgg gcgcgtcgcg
     1321 tgcctggatg cggccgtggt ggtcgagacc aagtcgagtg gtccgccgac gatgttcgac
     1381 cgggtgttgt ggcgccacgg gtgccggcct gcaccgatca gcaagttcgc ggtgggtatg
     1441 gcgttgctgg ttcccggttt gcccgccaac aagtggcacc gcacgctgaa ccgtccctcc
     1501 tgatccatcg ccacagatcg atcgccacag atcgatcgct gccgactggg cagtgctgac
     1561 ccgtctgtgc tgaagcgttg tcgccgactc ggtttgggtg acccggcgta ggctggcacc
     1621 gatggacgca gcgccacggg tcgccctcgc tctcggcagc ggtggcgcac gcggctacgc
     1681 ccacatcggc gtcatccagg tgttgcgcga acagggctac gagatcgctg gtgttgccgg
     1741 aagctcgatg ggtgcgcttg tcggcggtct ctatgccgtc gggcgcctcg atgactttac
     1801 ggactgggca ctcggcctca cccgcttcga cgtgttgcgc ctgatggacg tgtcgttcac
     1861 cgcgccgggc gcgatccggg ccgagaagat tctgaaccgg gtgcgcgatc tggccgaagg
     1921 ggtgttgatc gaggatctcc ccatcccgta caccgcggtg gccagcgatc ttggcgcgcg
     1981 caaggcggta tggttccaac gcggcccgct cgacgtggcg attcgcgcgt ccatcgcgat
     2041 tccgggggtg ttcgcgccgg tgatgctgaa cggccgcatc ctggtcgacg ggggcgcgat
     2101 ggacccggtg ccgatgctgg cgttggcagc gatccgggcc gacttcacca tcgccgtcga
     2161 cctcggaggc ttgcgctcgc ccgtcaccga cgagccgctc gaggtgtccg gtgagagccg
     2221 tccgatggag gaatgggccg acaagctgaa gcgcactgcg tcgaacctgt tcgaccgcga
     2281 tctggtgcga tcgctgatga gccgcctcga ccgcggccgc gacagcgccg acgacaccat
     2341 caacgacagc gccagcgaca gcatcaacga cagcgctggc gacagcgggg acaagcttgg
     2401 ctccgagatc gaggacgtcg acgtcggcat caccgacaag atcgctgacc tcagagtcca
     2461 cgaacgtgat catgagccgt tggctgtgtt cgaggnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngg ccaccaacgc
     2701 cctcgatcgc cgctgaagtc gccgctgccg tcacccactg gcagcgaggt gccgttcgtc
     2761 gtggcggcgc gtattacgat cgtccgaacg tgcacggaca agggtggaga ccatgagcga
     2821 cccgtacagc aatccccaga tctcaccgcc accgccaccg cagggctatg aacctgcggg
     2881 agcgccgctc aactttgccc caccgacacc gcctcagggt tcggcggggg gcgcggcgaa
     2941 cggcggcggt tggccggggg cggccaagtt caccgccatc ttgctgggcg cgttgctgat
     3001 cgccggtgga gtcgtcgcgt tcttgctggt gcgccaagca ggacgcggac gactccaagg
     3061 cggcgctgtc ggcggtcaac gagtcgctgt ccgacctcac cgaccaggcc gactccgacg
     3121 ccgattcggt caaggaccag atcgacgcgc tgctcgacca gcaggcgacc gacaaaacca
     3181 cgcagcagca gctacaggcc acgatcgccg acctcaacga gcagatcacg accttgacca
     3241 accaagtggc caacggcgcc actggcggcg gcgaagacgt ggccgcgctg caaacgcagc
     3301 tcgaacaggc ccgccaggat ctcgccgacg tgcaggccca gctcacagag acccaggacg
     3361 atctggacga ggccctggcc aacgccaaca cgacggcggc accgttcgac gtcgaggcgg
     3421 gcaaggtacc cctcggcggt ggcaacttct ccgtcgtgac caaccgggtc acctgcgacg
     3481 gctacgccga tgcggccagc tcctgccccg acacgttcac cgtcgacggc cggatcttct
     3541 ccgaaggcga cggatacgtg atcgaattcg ccaacatcgc cagggtctcg atgggctcct
     3601 tcgacggttt caactacggg ggccaggcac cggcaacggg cgacgtcagc ccgacctgca
     3661 acggcaaccc ggttgccacc accttcgccg tgcagatcgc gccggtgcgc taccacgtcg
     3721 acccggcgac ccaagcggtg tcgtcgaccg cgttcagttt cacctggacc atgtcggcgc
     3781 cggccggggg cggttgcgca gccaccactc gggtctacac cggcaccatc gccctctgat
     3841 ccccgccgcc cggtccgggt ggcccggtgg gtggcgcggg tcccgatcgg tgccccgacc
     3901 tggtgaacgt cgcgccgatc gatagcctcc ctgcgtgctc atcgccggac tttccgactt
     3961 cgtcacgggc gacctcaccg attgggtcgt tcgcgtcatc gaagccatca gctatctcgg
     4021 tgtcgccttg ctggtcgcgc tcgagagcgt gtttccgccg atcccgtccg aggtcgtgct
     4081 gccggccgcg ggtctcaccg cattccgggg tgacgccaac ctgatcggca tgatggtggc
     4141 ggccacggtc gggtcggtgg tcggggcgtg ggtgctctac ctcatcgccg cggccatcgg
     4201 acatcgccgc ttgcacgcgt tggtggtccg ctacggcaag tggctgggag tgaaggagaa
     4261 ggatctgctg cgagccgagt cgtggttcga cgaccatgcc aacacggcag tgttgatctg
     4321 ccgttgcatc cccctgatcc gttcgttggt ctcgatccca gccgggttcc ggcgaatgaa
     4381 cgccctccag ttcacgatct acaccaccgt cggcagcgcg gtgtggaacg tggtgctgat
     4441 cggcgccgga taccgcctgg gcgaccactg ggagcgtgtc ggcgactggg tcggcgtgct
     4501 gcagtacgtg gtcgttgcgg cgatcctggt ggtcctcgcg atctgggtgt ggcgccggtt
     4561 catctccccg acccacaagg cccgtcgccg ggcagaagaa ctgcaggagg cgatgcagat
     4621 ccaagtcgcc gaggccatcc tcgacgccac cgactgagtc ggaggacagt ccgggcccca
     4681 gggggccgac tcgccgcggg gactgaagca tcgtcatacg atgtttgtcg ggcgtgcgtt
     4741 gggacggttc gagtagctct gcctacgatg gccgccgatg gtgaagcgat cgacggtgaa
     4801 gcgcaagatc tggcgggctc tgacgctgcg gtgcccgtgg tgcggcagcc gcaagacgtt
     4861 catcctgcaa taccgcatcg gccggcgctc gcgcaccatg agcctcgggt acacgcgagt
     4921 gatcacgccg gacaacgcgc gcggcatggc gatcgcgcac ctcgccgcga tcgagaacaa
     4981 ccgcgatcct ctggccgacc gcgacgacca ccgcgacgcg ctcagtttcc gagagctcgc
     5041 catgcggttc gatgccgagc atatcgccgt gcacatcaag cccagcacgg gcgccgagta
     5101 ccggagggcc ctgaagaaag ccatcctgcc gttcctcggc cggcgcccgg tcgctgaggt
     5161 gagccgcgag gaaatcgccc gcttccgcca caagtaccgg gacgtgccgt acttcgccaa
     5221 ccgctgcctc gccatcctct cgaagatgtt caatctcgcc gagctgtggg ggctgcggcc
     5281 ggatggcagc aacccttgcc ggcacatcag gaagtacaag gaggagcggc gggagcggtt
     5341 tctctccgcc gccgagctgc gccgtgtcgg ccaagtgctc gccgagatgg tggcggagcg
     5401 ggtggaaatg ccctcggcgc tgaccgccat ccgnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5461 nnnnnnnnnn nnnnnnnnnn nnnnnnnncg gcgccctcaa tctcccagac tcgaagaccg
     5521 gcgccaaggt tgtctacctc ggccagccgg cgctcgatgt cctgcgcagc gccccgcgcc
     5581 tacccggcaa cccgtgggta atctggggca agaagcacgg cacgcacctc tacggccttc
     5641 aggccgtctg ggagcgggtg cgcgcccgcg ccggtctcaa ggatgtccgc atccacgatc
     5701 ttc
//
LOCUS       DAORNF010000120         5700 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_10458_length_5700_cov_1.679539, whole genome shotgun sequence.
ACCESSION   DAORNF010000120 DAORNF010000000
VERSION     DAORNF010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 5700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5700
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10458_length_5700_cov_1.679539"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>323
                     /locus_tag="PLV68_06245"
     CDS             <1..>323
                     /locus_tag="PLV68_06245"
                     /inference="COORDINATES: protein motif:HMM:NF015129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00880.1"
                     /translation="PQVLAGFGVDERVAWLELDLTRLLANEPKIAQWKPISRYPSSDF
                     DLAFVVKNDVTAERMEKAIRQAAGGLLVGIELFDVYRGAGVADDARSLAYRLWLQALD
                     RTLTD"
     assembly_gap    324..435
                     /estimated_length=112
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<436..1049)
                     /locus_tag="PLV68_06250"
     CDS             complement(<436..1049)
                     /locus_tag="PLV68_06250"
                     /inference="COORDINATES: protein motif:HMM:NF014659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HRE00881.1"
                     /translation="MSIGRAFIQGSLLVAVPLLDDPNFHRSVVYVLQHDDDGALGVII
                     NRPTPTRSGLDNLFGMDAWLDELSPPRVVFEGGPVQINSLIAIAATDHATITGPDAAR
                     PGVASPDVASPEIASPEIASPEIEGFAPLSPGLGTVDLTVLPASIAAYLQHLRVFRGY
                     SGWGPGQLEDELDQGAWLPVAADRGDLFTTNPADLWRNVLRRAGG"
     gene            1192..2211
                     /gene="argC"
                     /locus_tag="PLV68_06255"
     CDS             1192..2211
                     /gene="argC"
                     /locus_tag="PLV68_06255"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HRE00882.1"
                     /translation="MVRVGIIGASGYTGVELLRLVAQHPEFELVVATGDTQAGTRAAS
                     LYPSLAVQYPDLVFSEFDAEVAAGLDLVFLGLPHEASMALAPALVGRVGCVVDLSAAY
                     RLRDASLYPRWYGFSHDQPELLAQAVYGLPELYRDQLRGAALIATPGCHVTAASLALA
                     PLVAAGLIEPTGIIVNSATGISGAGRSLKHASLFCTVDENYEAYGLLDHRHTPEIEQV
                     VGAQVLFTPHLTPMNRGILATCYARPGDPALRTEIVIGALRDAYAGEPFVAVGTESPG
                     TKATLGSNAAHLTARYDERTGTVIALCAIDNLAKGASGGAVQAANVALGLPETMGLTA
                     VGLLP"
     gene            2208..>2891
                     /gene="argB"
                     /locus_tag="PLV68_06260"
     CDS             2208..>2891
                     /gene="argB"
                     /locus_tag="PLV68_06260"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HRE00883.1"
                     /translation="MSVAEQPLATPASTPGVKAGVLVEALPYIRRFAGKTVVVKYGGN
                     ALAGASDSDALALFAQDIVMMHAVGIRPVVVHGGGPQISELMTRLGKVTEFRNGLRVT
                     DAETVEIARMVLVGQVNPQIVATINVHGPLAVGVSGADAGLIVAEARDPELGYVGDVT
                     DVNPTILNRLLNEDLIPVVATIGSSVAGQPYNINADTVAGAIAEALGAEKLVYLTDIE
                     GLRRVVDDPA"
     assembly_gap    2892..2901
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2902..3158
                     /locus_tag="PLV68_06265"
     CDS             <2902..3158
                     /locus_tag="PLV68_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00884.1"
                     /translation="IRQTTPDELDALMADGTIAGGMIPKVESCVHAVRHGVRRAHILD
                     GRIPHVLLLEIFTDDGIGTMVKPSTLVSALLASEPPGVLA"
     gene            3155..>3771
                     /locus_tag="PLV68_06270"
     CDS             3155..>3771
                     /locus_tag="PLV68_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRE00885.1"
                     /translation="MSSVFSPQRPSPFMPVFAEPTVVFERGSGTELWDTDGKRYLDFL
                     GGLAVIGLGHAHPAIADALAAQSRTLLHVSNLFANDVAIDAATMVDDLLREATGADGL
                     TFFCNSGAEANEGALKLARKFGGRGRHTVVSAYGSFHGRTLATLAATGQPAKHEPFFP
                     MPDGFRHVAYGDIDALAASVDPSVAAVLIETVQGEGGVVPAPAGYL"
     assembly_gap    3772..3923
                     /estimated_length=152
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3924..4374
                     /locus_tag="PLV68_06275"
     CDS             <3924..4374
                     /locus_tag="PLV68_06275"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRE00886.1"
                     /translation="FPVGAVWARTEVANAFQPGDHGSTYSATALAAAVVRAVITEMRR
                     IDAPALAARQGARLADALATVPGVVAVRGQGLLLAAELAPGIDAKMAYAALLERGVVC
                     NAVTPTALRFAPPLTVRDAEIDEVVAMLTAVLADLAAPAESATGVVS"
     assembly_gap    4530..4578
                     /estimated_length=49
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4579..5291
                     /locus_tag="PLV68_06280"
     CDS             <4579..5291
                     /locus_tag="PLV68_06280"
                     /inference="COORDINATES: protein motif:HMM:NF012412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HRE00887.1"
                     /translation="GEEVGFDVREAVEDVTRIMAGYHHVLAARVFDHAVVQRMAAVSA
                     VPVVNMLSDHSHPLQALADTLTMTTEFGDLADVTVAYVGDYNNVARSLAEACVMLGAH
                     VRFGCPVGYDASDAELARLDALGVGTVEQSSDPFVAVRGADAVHTDTWTSMGQESEKA
                     ARAEVFGHYQVNDGLMAAASSRAIFMHCMPAYRGLEVAADVIDGPRSRVFAQGHNRMH
                     SARGVLAFLTGAVSVPAG"
     gene            5302..>5700
                     /locus_tag="PLV68_06285"
     CDS             5302..>5700
                     /locus_tag="PLV68_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HRE00888.1"
                     /translation="MTTKVQRQQTVVRLIGRHPVTSQPQLLELLAAEGITATQDTVSR
                     DLEDLGAVKVRVPGGDTVYAIPEYEPNRIAPEDQLRRVMGEWVAEVRHAHHNVVIRTP
                     PGCAHVVASALDRSGLTGVLGPVAGDDTLLV"
BASE COUNT          817 a         1835 c         1878 g          847 t
ORIGIN      
        1 acccgcaggt gctggccggc ttcggcgtcg acgagcgcgt cgcgtggctc gagctcgacc
       61 tcacccggtt gctggccaac gagcccaaga tcgcccagtg gaagccgatc agccgctacc
      121 cctcgtcgga cttcgacctg gctttcgtgg tcaagaacga cgtgaccgcg gaacggatgg
      181 agaaggcgat ccgccaggcc gccggcggcc tgctggtcgg catcgagctg ttcgacgtgt
      241 accgcggtgc gggtgtggcc gacgacgccc gcagcctggc ctaccggctg tggctacagg
      301 cgctcgaccg cacgctgacc gatnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnccgcc ggcacggcgc agcacgttgc gccacaggtc ggccgggttg
      481 gtggtgaaca ggtcgccgcg atcggcggcg accggcagcc acgccccctg gtcgagctcg
      541 tcttcgagct ggccgggtcc ccatccggaa tagccgcgga acacgcgcag gtgttggagg
      601 taggcggcga tcgaggcagg cagcacggtg agatcgaccg tgcccagccc cggactgagt
      661 ggtgcgaagc cctcgatctc cgggctggcg atctccgggc tggcgatctc cgggctggca
      721 acatcggggc tggcaacgcc ggggcgggca gcgtcaggtc cggtgatcgt ggcgtgatcg
      781 gtagccgcga tcgcgatcag cgagttgatc tgcaccgggc cgccttcgaa caccacgcgc
      841 ggcggtgaca gttcgtcgag ccacgcgtcc atgccgaaca ggttgtcgag accggatcgt
      901 gtgggggtgg ggcggttgat gatcacgccc agcgcgccgt cgtcgtcgtg ttgcagcacg
      961 tagaccacgc tgcgatggaa gttgggatcg tccaacaggg gtacggcgac gagcagagag
     1021 ccttggatga aggcacgacc gatgctcacc tggccattct gcttcgctcg ctgtcgctgt
     1081 gccagccaac gcgggccagc caacgcggct gggtgatcaa cacagccgat caccgggtca
     1141 tgtccgaatg gtgttgcatg aacatgcaca gaactgtatg gtcatgcgcc tatggttcgg
     1201 gtcggcatca tcggcgccag cgggtacacg ggagtcgagc tcctccgcct ggtcgcgcag
     1261 catccagagt tcgagttggt ggtggccact ggcgacaccc aggcgggtac ccgtgctgcg
     1321 tcgctgtacc cgtcgttggc ggtgcagtac cccgatctcg tcttcagcga gttcgacgcc
     1381 gaggtagcag ccggtctcga cctggtgttc ctcggtctgc cccacgaggc gtcgatggcg
     1441 ttggccccgg ccctggtcgg gcgggtcggc tgcgtggtcg acctgtcggc cgcttaccgg
     1501 ttgcgcgacg cctcgctcta cccgcgctgg tacggcttct cgcacgacca gccggaactg
     1561 ctcgcccagg cggtgtacgg gctgcccgag ctctaccgcg accagctgcg cggcgcggcg
     1621 ctgatcgcca cgcccggctg tcacgtcacc gcggccagcc tggccctcgc cccgttggtc
     1681 gcagcggggc tgatcgaacc gaccgggatc atcgtcaaca gcgccacagg catcagcggc
     1741 gccggtcgct cgttgaagca cgccagcctg ttctgcacgg tcgacgagaa ctacgaggcg
     1801 tacgggttgc tcgaccatcg gcatacgccc gagatcgaac aagtcgtcgg cgcgcaggtg
     1861 ctgttcacgc cgcatctgac gccgatgaac cgtggcatcc tggccacgtg ctacgcccgc
     1921 cccggcgatc ccgcgctgcg caccgagatc gtgatcggcg ccttgcgcga cgcgtacgcc
     1981 ggtgaaccgt tcgtcgcggt cggtaccgag tcgcccggca ccaaggccac gctcggctcc
     2041 aacgcggcac atctcacggc gcgctacgac gagcgcaccg gcaccgtgat cgcgctgtgc
     2101 gcgatcgaca acctggccaa gggtgccagc ggtggagcgg tccaggccgc caacgtcgcc
     2161 ctcgggctgc cggagacgat gggcctgacc gccgtcgggc tgctgccgtg agcgtcgccg
     2221 agcaaccgct ggccacgccg gcctcgacgc caggcgtcaa agcgggggtg ttggtcgagg
     2281 cgttgcccta catccgccgc ttcgcgggca agaccgtggt ggtcaagtac ggcggcaacg
     2341 cgctggcggg cgcgagcgac agcgacgcac tggcgttgtt cgcgcaggac atcgtgatga
     2401 tgcacgccgt cggcatccgc cccgtcgtcg tccacggtgg cggcccgcag atcagcgagc
     2461 tgatgacccg gctgggcaag gtgaccgagt tccgcaacgg cctgcgggtg accgacgcgg
     2521 agacggtcga gatcgcccgg atggtgctgg tcgggcaggt caacccgcag atcgtggcca
     2581 cgatcaacgt ccacggtccg ctcgcggtcg gcgtctctgg cgctgatgcg ggtctgatcg
     2641 tggccgaagc gcgtgatccc gaactcggct acgtcggcga cgtcaccgac gtcaacccca
     2701 cgatcctcaa ccggttgctg aacgaggacc tgatcccggt ggtggccacg atcggatcgt
     2761 cggtggcagg tcagccatac aacatcaacg ccgacacggt cgcgggtgcg atcgccgagg
     2821 cactcggcgc cgagaagctg gtgtacctga ccgacatcga agggctgcgc cgcgtcgtcg
     2881 acgacccggc cnnnnnnnnn ntcattcgcc agacgacgcc cgacgagctc gacgcgttga
     2941 tggccgacgg cacgatcgcg ggcgggatga tccccaaggt cgagagctgc gtccacgctg
     3001 tgcgccacgg cgtgcgccgg gcccacatcc tcgacggccg catcccccac gtgctgctgc
     3061 tggagatctt caccgacgac ggcatcggca cgatggtcaa accgtcgacg ctcgtctcgg
     3121 ccctgttggc ctctgaaccc cctggagtcc tcgcatgagt tccgtgttct caccgcagcg
     3181 tccgagcccg ttcatgccgg tgttcgccga gccgacggtg gtgttcgagc gtggctcggg
     3241 taccgagctg tgggacaccg acggcaagcg gtatctcgac ttcctcggtg ggttggccgt
     3301 gatcggactc ggtcacgccc atccagcgat cgcggatgcg ctggcggcac agagccgcac
     3361 cctgttgcac gtcagcaacc tgttcgccaa cgacgtcgcg atcgacgcgg ccacgatggt
     3421 cgacgacctg ctgcgcgagg ccaccggcgc cgacgggttg acgttcttct gcaactccgg
     3481 cgccgaagcc aacgagggtg ccctgaagct ggcgcgcaag ttcggtgggc gggggcggca
     3541 caccgtcgtg tcggcctacg gcagcttcca cggccgcacg ctcgccaccc tggcagccac
     3601 aggccaaccg gccaagcacg aaccgttctt cccgatgccc gacgggttcc gtcacgtcgc
     3661 ctacggcgac atcgatgcac tggccgcgtc ggtcgacccg tcggtcgcag cggtgctgat
     3721 cgagaccgtg cagggtgaag gcggtgtcgt gcccgcccct gccgggtacc tnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnnnnnn nnnnnnnnnn nnnattcccg gtgggcgcgg tgtgggcacg caccgaggtc
     3961 gccaatgcgt tccagcccgg cgatcacggc agcacctaca gcgcaacggc gctcgccgcg
     4021 gcggtggtgc gggcggtgat caccgagatg cgccgcatcg acgccccggc gctcgcggcc
     4081 cgccaaggcg cacgtttggc cgatgcgttg gcgacggtgc cgggtgtggt cgccgtgcgc
     4141 ggccagggct tgctgctggc cgccgaactg gcgccgggca tcgatgccaa aatggcgtac
     4201 gcggccttgc tcgaacgcgg cgtcgtctgc aacgcggtca ccccgacggc attgcgcttc
     4261 gcgccgccgc tcaccgtgcg cgacgccgag atcgacgagg tggtcgcgat gctgaccgcg
     4321 gtgttggccg atctggcggc gcccgccgaa tccgctaccg gggtggtctc gtgagcgcac
     4381 gttacttcct gcagatcacc gatctcagca cggacgaact gcgcgcggtg ctcgcgctga
     4441 gcaccgcgcc gatcgcttcg ctgggtgcgc cgttggccgg caagggtgcc gcgctgatct
     4501 tcgagaagcc gtcgagccgt acccgccacn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nnnnnnnngg ggcgaggagg tcggctttga cgtgcgcgaa gccgtcgaag
     4621 acgtcacccg gatcatggcc ggctaccacc acgtgctggc cgcccgcgtg ttcgaccacg
     4681 ccgtggtgca gcggatggcg gcggtcagtg cggttccggt cgtcaacatg ctcagcgacc
     4741 acagccaccc gctgcaggcg ctggccgaca cgttgacgat gaccaccgag ttcggcgacc
     4801 tggccgacgt gacggtcgcc tacgtcggcg actacaacaa cgtcgcccgc tcactggccg
     4861 aggcgtgcgt catgctgggt gcccacgtgc gcttcgggtg cccggtgggc tatgacgcca
     4921 gcgacgccga gttggcccgg ctcgacgcgt tgggggtggg cacggtcgaa cagtcgagcg
     4981 atccgttcgt ggctgtgcgt ggcgccgatg cggtgcacac cgatacctgg acctcgatgg
     5041 ggcaagagtc cgagaaggcg gcgcgagcgg aggtgttcgg gcactaccag gtcaacgacg
     5101 ggctgatggc cgcggcatcg tcgcgggcga tcttcatgca ctgcatgccc gcctaccgcg
     5161 ggctcgaggt cgcggccgac gtcatcgacg ggccgaggtc gcgagtgttc gcgcaaggcc
     5221 acaaccggat gcactccgcc cgcggcgtgc tcgcgttcct gacgggcgcc gtatcggtgc
     5281 cagcagggtg agaggggagc catgaccacc aaggtgcaac gccagcagac ggttgtccgc
     5341 ctcatcggcc gtcaccccgt cacgagccag ccgcagttgc tcgaattgct cgccgccgaa
     5401 ggcatcaccg ccacgcagga cacggtgtca cgcgacctgg aagatctcgg tgcggtcaag
     5461 gtacgggttc ccggcggcga caccgtgtat gcgatccccg agtacgaacc gaaccggatc
     5521 gcccccgaag accaactgcg ccgggtgatg ggcgagtggg tggccgaggt gcgccacgcc
     5581 caccacaacg tcgtcattcg cacgccgccc gggtgtgccc acgtggtcgc atcagcgctc
     5641 gaccgctctg gactgactgg ggtgctcggc cccgtcgccg gagacgacac gctgctggtg
//
LOCUS       DAORNF010000121         5695 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_10478_length_5695_cov_1.823227, whole genome shotgun sequence.
ACCESSION   DAORNF010000121 DAORNF010000000
VERSION     DAORNF010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 5695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5695
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10478_length_5695_cov_1.823227"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1910)
                     /locus_tag="PLV68_06290"
     CDS             complement(<1..>1910)
                     /locus_tag="PLV68_06290"
                     /inference="COORDINATES: protein motif:HMM:NF039608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00889.1"
                     /translation="TDTGCVDLPNVTIAKTAPATATATVNPGEFTTTYTVTVTNKGPA
                     TTYNLVDAFDFSATVQVVSGSATVSYGVGATSVAGYDGTSNTTLATAQAIAAGTPANP
                     VVHTYLVTTVVKLDGNETPAQLSCADGGPDHGLYNLATLTVAGRPSTDDACSDVPTRS
                     VSLVKSVSGVAGAYTWSFSFDISPLPNGQSGPKTINGTGPNTSEPISWTNLIPGLTYT
                     VSEAAVGGWGQMLTCTGVDDSDDQTDNSVTFVAPTSDTAISCTATNTATTPTLKVVKT
                     TVGGYGTFNFDVTNLTGGPIAVTTAAGTNPATSATRNLLTGTLYTVSEQADAAWTPGT
                     LTCTVTPAGSQQAQAIDGTSFTAEPGAAYTCQITNTRKATVVVNKVVQGGDGSFGFSG
                     SLPSLPSTIAATVGTPGTYTAANVTPGAYTVTEAAAPGWDLTSLVCDDGQSTVPSTVS
                     GRTATINAEAGETVTCTFTNAKQGGITIVKDAVPNHAQDFSFTTTSTATTIDASFSLD
                     DDAGAAGANDTLANSASFTSLSAGTYTFTEGQVAGWTLTAINCSGATATPNVGDRTVS
                     IVLPAGGSATCTFVNTAATPTLTVTKTTVGGTGTFTFDVTGLTDADGLSVSTVAANPN
                     TSAAQNLAVGTA"
     assembly_gap    1911..1924
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1925..>2170)
                     /locus_tag="PLV68_06295"
     CDS             complement(<1925..>2170)
                     /locus_tag="PLV68_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00890.1"
                     /translation="SLVPTVFTLTDQLLADAGVTGTFTWDAATPQPAADTPIQPGAAN
                     KLTYTIHASFTVDPAAPGFQYECGSESNPTGGLANQAS"
     assembly_gap    2171..2280
                     /estimated_length=110
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2281..>3514)
                     /locus_tag="PLV68_06300"
     CDS             complement(<2281..>3514)
                     /locus_tag="PLV68_06300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00891.1"
                     /translation="QPVYSGGVWTVVYTVTVKHAAGSKVPTQYTLSDTPSAGSGITIT
                     SSSSGTETSTDSPFTLTLATAKSLAVGATDTYDVTVTYTTGANPDLTCATGGAKNTAT
                     VTWSGGTDNDTACAPIPSVSLTKTAPATASATATPGEFTTTYTVTVSNKSAATTYNLN
                     DAFDFAGSIEVLGVDSVGFVAAPVAVVDNPTFTANTSGVTATAVPIAAGTSANPTTHT
                     WTITVRVRLNGDESSDAVTCAANDTADRGLFNKATVTVQGYSADDTACSNLPKPAITV
                     VKTPDSGVTATGPAGGPFTATYTVTVTNNGTAPGSYVLKDVPAAVSGTTITNIAITSA
                     SPAVNVPNYTSGTALPIQAIAASTAHVYTVTVTFTVATVTAANVVNFTCAEGTPVAGK
                     GQVNTATITVAGTDSSDTG"
     assembly_gap    3515..3564
                     /estimated_length=50
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3565..>3880)
                     /locus_tag="PLV68_06305"
     CDS             complement(<3565..>3880)
                     /locus_tag="PLV68_06305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00892.1"
                     /translation="TYTLVDTPAFDPSTTVSKVELISSSPSITAPAPGTTSLTVVSGR
                     AIAGASPTAVTHTYRVRVTFSFTGSTSTTASRDCTKETGESGTGTFNVATVTPNIGNP
                     SSD"
     assembly_gap    3881..3963
                     /estimated_length=83
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3964..>5695)
                     /locus_tag="PLV68_06310"
     CDS             complement(<3964..>5695)
                     /locus_tag="PLV68_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00893.1"
                     /translation="EFATNARRAVVNGDTEFQTVNAAYVNTLTTTYLRNLADTNSSIA
                     LTDPNTYNPYQNAAGGTNPQYTNWEAGLYQALDGAPLVVFITDGDPNTVGTTGSSTAN
                     NLPNSDLAASAAVGIMNQLKENGQHVLAIGVGNGVTNTTSFNRLRDLVEPNNPSVWEG
                     GAGSFDIRTTDALKVTDFSALTDALRRVVFSLCAPALTLNKVDGDNAAVANHDFTTTI
                     SDVNSGGATKQVDWITPDEQIQTVPTSQTVATNASGSSLFQWNPNTRSDPKPWASTVS
                     FSEVMPPGWTAGWSAGSLPSCSVQRLKTDGTISDFTVTVTPSPMVPTGGQTVVFTFNT
                     NNAAGFDGKIEAGDLVNCTIKNQRPPSLTVTKTTVGGTGSFTFDVTNVATNVGSPITV
                     ATATAGTLVTSTSTQLVTGTSYNVTERDAAGWSKGTLTCTKDPAGPAGPAAVDLPFDA
                     EAGAKYNCSITNTKQASLTVTKTSYDGVGTFEFVVTNLPSSPISVTTATVGTPVTSAG
                     HTLTAGTTYTVSESDQAQWVEGSLACTQTLPGGSPTNVGTTFTAAPGADYNCAVTNRK
                     PNITVTKTPVT"
BASE COUNT          715 a         1601 c         2108 g         1014 t
ORIGIN      
        1 taggcggtgc cgacggccag gttctgggct gcgctggtgt tggggttggc ggcgacggtc
       61 gacaccgaca ggccgtcggc gtcggtcagg ccggtcacgt cgaaggtgaa ggtgcccgtg
      121 ccaccgacgg tggtcttggt caccgtcagc gtcggcgtgg cggcggtgtt gacgaaggtg
      181 cacgtcgccg agccgccagc aggcagcacg atgctgaccg tgcggtcgcc gacgttgggc
      241 gtcgcggtgg cgcccgagca gttgatcgcc gtcagggtcc acccggcgac ctggccttcg
      301 gtgaaggtgt aggtgcccgc cgacaggctg gtgaacgacg cggagttggc cagcgtgtcg
      361 ttggcacccg cggcaccggc gtcgtcgtcg agcgagaagc tggcgtcgat cgtggtggcc
      421 gtgctggtcg tggtgaagct gaagtcctgg gcgtggttgg gcaccgcgtc cttgacgatg
      481 gtgatgccgc cctgcttggc gttggtgaag gtgcacgtca ccgtctcgcc cgcttcggcg
      541 ttgatcgtgg ccgtgcggcc cgacaccgtc gagggcaccg tcgactggcc gtcgtcgcag
      601 accagcgacg tgagatccca accgggcgca gcagcctcgg tcaccgtgta ggcgccaggg
      661 gtgacgttgg ccgcggtgta ggtgccgggg gtaccgacgg ttgcggcgat ggtgctgggc
      721 aggctgggca ggctgccgct gaagccgaag ctgccgtcgc cgccttggac caccttgttg
      781 acgaccacgg tcgccttgcg ggtgttggtg atctggcagg tgtaggccgc gccgggttcg
      841 gcggtgaagc tcgtcccgtc gatcgcctgg gcctgctgcg atccagcggg cgtgaccgtg
      901 caggtcagcg tgccgggcgt ccaggcggcg tcggcctgct cggacacggt gtacagcgtt
      961 cccgtcaaca ggttgcgcgt cgcgctggtc gccgggttgg tgcccgccgc agtggtgacc
     1021 gcgatcgggc cgccggtcag gttggtgacg tcgaagttga acgtgccgta gccgcccacc
     1081 gtggtcttga ccaccttgag ggtcggggtc gtcgcggtgt tggtggcggt gcaggagatc
     1141 gccgtgtccg acgtgggggc gacgaaggtg accgagttgt cggtctggtc atcgctgtcg
     1201 tccacaccgg tgcaggtcag catctggccc caaccaccga ctgcggcctc gctgaccgtg
     1261 taggtcaggc cggggatcag gttggtccac gagatcggct ccgaggtgtt gggcccggtg
     1321 ccgttgatgg tcttggggcc gctctggccg ttgggcaacg gcgagatgtc gaagctgaag
     1381 ctccacgtgt aggcaccggc gacaccgctg accgacttga ccagcgacac cgaacgggtc
     1441 ggcacgtcac tgcaggcgtc gtcggtggac ggccgtccgg caaccgtcaa cgtggccagg
     1501 ttgtacaggc cgtgatcggg gccgccgtcg gcgcagctca gctgggccgg ggtctcgttg
     1561 ccgtcgagct tgaccaccgt cgtgacgagg taggtgtgca ccaccgggtt ggccggcgtg
     1621 ccggcggcga tcgcctgcgc tgtagcgagg gtggtgttcg aggtgccgtc gtacccggca
     1681 accgacgtcg cgccgactcc gtagctgacg gtggcgctac cgctgacgac ctgcacggtg
     1741 gccgagaagt cgaacgcgtc caccaggttg taggtggtgg cgggaccctt gttggtcacg
     1801 gtcaccgtgt aggtcgtggt gaactcaccc gggttcacgg tggccgtggc ggtcgcgggg
     1861 gccgtcttgg cgatggtgac gttgggcaga tcgacacagc cggtgtcggt nnnnnnnnnn
     1921 nnnngaggcc tggttggcca gaccgccggt cgggttcgac tcgctgccgc actcgtactg
     1981 gaagccagga gcggcggggt cgaccgtgaa cgacgcgtgg atcgtgtagg tcagcttgtt
     2041 cgcggcgccg ggctggatcg gcgtgtcggc ggcgggttgc ggcgtggccg cgtcccacgt
     2101 gaaggtgccg gtgacacccg cgtcggccag cagctggtcg gtcagcgtga acacggtcgg
     2161 caccaggctg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 agccggtgtc gctggagtcg gtgccagcga cggtgatggt cgcggtgttg acctggccct
     2341 tgccggcgac gggtgtgccc tcggcgcagg tgaagttgac gacgttggcg gccgtcaccg
     2401 tcgccacggt gaaggtcacc gtgacggtgt agacgtgagc cgtactggcc gcgatcgctt
     2461 ggatcggcag ggccgtgccc gaggtgtagt tgggcacgtt cacggccggg ctggcggagg
     2521 tgatcgcgat gttggtgatc gtcgtgccgg agacggccgc aggcacgtcc ttcagcacgt
     2581 agctgccggg ggcggtgccg ttgttggtga ccgtcaccgt gtaggtcgcc gtgaacgggc
     2641 caccggccgg gccggtcgcg gtcacgccgc tgtccggggt cttgacgacg gtgatcgccg
     2701 gcttgggcag gttcgagcag gcggtgtcgt cggcggagta accctggacc gtgacggtgg
     2761 ccttgttgaa caggccgcgg tcggcggtgt cgttggcggc gcaggtcacg gcgtcgctgg
     2821 actcgtcacc gttgagacgg acccgcaccg tgatcgtcca cgtgtgcgtc gtggggttgg
     2881 ccgacgtgcc agccgcgatc ggcaccgcgg tcgcggtgac gcccgacgtg ttcgccgtga
     2941 aggtggggtt gtcgacgaca gcgaccggtg cggcgacgaa gcccaccgag tcgacgccga
     3001 gcacctcgat gctgccggcg aagtcgaacg catcgttgag gttgtacgtg gtggccgcgc
     3061 tcttgttcga cacggtcacg gtgtaggtgg tggtgaactc acccggcgtc gcggtggccg
     3121 acgccgtcgc cggcgcggtc ttggtgaggc tgaccgacgg gatcggcgca cacgccgtgt
     3181 cgttgtcggt gccgccgctc cacgtgaccg tcgcggtgtt cttggcaccg ccggtagcgc
     3241 aggtgaggtc ggggttggca ccggtggtgt aggtgacggt gacgtcgtag gtgtcggtcg
     3301 cgccgacggc gagactcttc gccgtcgcca gcgtgagggt gaacggcgag tctgtcgacg
     3361 tctccgtgcc cgaggacgac gacgtgatcg tgatgcccga acccgcgctg ggggtgtcgc
     3421 tcagcgtgta ctgcgtcggc accttggaac ccgccgcgtg cttgacggtg acggtgtaga
     3481 ccacggtcca cacgccgccc gaatagaccg gctgnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnngtcgct ggaggggttg ccgatgttcg gcgtgacggt
     3601 ggcgacgttg aacgtgccgg tgccggactc gccggtctcc ttggtgcagt cacggctggc
     3661 cgtggtggac gtggagccgg tgaagctgaa cgtgacccgc acccggtagg tgtgcgtcac
     3721 cgcggtgggg ctggcccccg cgatcgcccg cccgctgacg acggtcagcg acgtggtacc
     3781 cggagcgggg gcggtgatgc tggggctgga cgagatcaac tcgaccttgc tgacggttgt
     3841 cgacggatcg aacgccgggg tgtcgaccaa ggtgtacgtg nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnggtgaca ggcgtcttgg tgacggtgat gttcggcttg cggttggtga cggcgcagtt
     4021 gtagtccgcc ccgggcgctg cggtgaacgt cgtgccgacg ttggtcggcg acccacccgg
     4081 caaggtctgg gtgcaggcga gggaaccctc cacccactgg gcctggtcac tctcgctgac
     4141 cgtgtaggtc gtcccagcgg tcagggtgtg accggcgctg gtcacggggg tgcccaccgt
     4201 ggcggtcgtg accgagatcg gcgacgacgg caggttggtg acgacgaact cgaacgtccc
     4261 caccccgtcg tagctcgtct tggtcaccgt cagcgacgcc tgcttggtgt tggtgatcga
     4321 gcagttgtac ttggcaccgg cttcggcgtc gaatggcagg tcgaccgccg cgggtccggc
     4381 cggaccggca gggtccttgg tgcaggtcag cgtgcccttg ctccagccag cggcatcgcg
     4441 ctcggtgacg ttgtagctgg tgccggtaac caactgcgtc gacgtgctgg tgaccagggt
     4501 tccggctgtc gccgtggcga ccgtgatcgg cgaaccaacg ttggtcgcaa cgttggtgac
     4561 gtcgaaggtg aacgaaccgg taccgccgac cgtggtcttg gttacggtca ggctcggcgg
     4621 gcgctggttc ttgatggtgc agttgacgag gtcgcctgcc tcgatcttgc cgtcgaagcc
     4681 ggccgcatta ttggtgttga aggtgaagac cacggtctgg ccaccggtgg gaaccatcgg
     4741 gctcggcgtg accgtcacgg tgaagtcgga gatcgtgccg tcggtcttga ggcgctgcac
     4801 cgaacaggac ggcaaggagc cagcgctcca tcccgcggtc cagccgggcg gcatcacctc
     4861 gctgaacgac accgtcgagg cccacggctt ggggtcggag cgggtgttgg ggttccactg
     4921 gaacagcgac gagccggacg cgttggtcgc cacggtctgg ctcgtcggca ccgtctggat
     4981 ctgctcgtcg ggtgtgatcc agtcgacctg cttggtcgcg ccgccactgt tgacgtcgct
     5041 gatcgtggtg gtgaagtcgt ggttggcgac cgccgcgttg tcgccgtcga ccttgttcaa
     5101 ggtcagcgcc ggagcacaca gcgagaagac cacccggcgc agcgcgtcgg tcagcgcgga
     5161 gaagtcggtg accttgaggg cgtcggtggt gcgaatgtcg aagctgcccg cgccgccttc
     5221 ccacacgctc gggttgttgg gctcgaccag atcgcgcaag cggttgaagc tggtggtgtt
     5281 ggtgacgccg ttgccgacgc cgatcgccag cacgtgctgg ccgttctcct tcaactggtt
     5341 catgatgccc acggcagcag aggcggccag gtccgagttg ggcaggttgt tggctgtgga
     5401 ggaaccggtg gtgccgaccg tgttcgggtc gccgtcggtg atgaacacca ccagcggtgc
     5461 accgtcgagg gcttggtaca gacccgcctc ccagttggtg tactgggggt tcgtgccgcc
     5521 ggcagcgttc tgatacgggt tgtacgtgtt ggggtcggtg agcgcgatcg aactgttggt
     5581 gtcggccagg ttgcgcaggt aggtggttgt caacgtgttg acgtaggcgg cgttcacggt
     5641 ctggaactcg gtgtcgccgt tgacgaccgc gcggcgggcg ttggtcgcga actcg
//
LOCUS       DAORNF010000122         5689 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_10494_length_5689_cov_1.751686, whole genome shotgun sequence.
ACCESSION   DAORNF010000122 DAORNF010000000
VERSION     DAORNF010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 5689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5689
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10494_length_5689_cov_1.751686"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>375
                     /locus_tag="PLV68_06315"
     CDS             <1..>375
                     /locus_tag="PLV68_06315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00894.1"
                     /translation="QLGDLVHLADGPNKLQNVTIQFDAWACQRGDRTTCDTDPGATFA
                     HPITVTIYSADLSTKLAEVTEIVDVPFRPTRDATCPALSSEPAGSGWRADDGECYNGY
                     GFRWTFDFPSGPVLPSDVVWTV"
     assembly_gap    376..390
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            573..>3122
                     /locus_tag="PLV68_06320"
     CDS             573..>3122
                     /locus_tag="PLV68_06320"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HRE00895.1"
                     /translation="MLRPTYICWGGYRPMATIRTTGPITHESETVTVTSDALHGWGWL
                     TDGNNPNATVTPRFTTATDTSGEDGSGSYPALQANVPIIVATNDYDGVKLADLTALSY
                     DTYSSRVDYAPSLALDVRLNSSNVWQGRLVFIASGDTSGWKTWDTFEPTAGLWYASNA
                     TASGGNCRDTLCTWDEVLTNWPDAMIDNPDGSSPGRLSVRAGTTDPDYSAATFHVDHI
                     VVGAMSAGPGTLTNTVTTFDFEPQLPTVPRFENATPGDGRALLKWTRPVSDGGLPITG
                     YVVTTPDGGTCTVSGTSALCTGLTNGSPYGFTVAAVNDLGTGPAVAIGPVTPAEHAVQ
                     VVAYDSIGLNPQGNYASLGYQATQTSEFGDLVEFAPGPRTLDDITVQFSSWACETGSG
                     TSCLTTPGSTFDHPITVTVYAVDESGPTPVVGAVLATMTKNVAVPYRPSANATCAGGT
                     TWKAANGTCYNGFAFTERFDFPEGITVPDRVIWAVSYNTQTWGYDPIGLPGPYTSLNV
                     GADSRGSATAFVGTDVNTDTVFWATRHAPFYCDDGAGGTNTLREDCDWTGNRPMATIR
                     TQGGATDTTVELTVTRDALQGWEWLTDGNNPNVAVPAVFTADTNTSGELGSGSYPTLA
                     PNEPVIVSTNAYDGVKLADISELGYETYSSRSDYAPGLSLDVKLTPSATTWQGRLVFV
                     PSGTTNGWQTWDTLDATNGLWYASNQTAAAAAGRCGLSALCTWDQVLTYWPQAQVGNP
                     DTNNNPLAPGRLSVRAGITATDYSADTFHVDHVVVGVVATGVSPVTNTITTYDFEPSL
                     PAAPTAVIAVAGDTTATVSWTAPDSGGTPLQSYTVTSAPGAKTCTATAPATT"
     assembly_gap    3123..3295
                     /estimated_length=173
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3296..>3536
                     /locus_tag="PLV68_06325"
     CDS             <3296..>3536
                     /locus_tag="PLV68_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00896.1"
                     /translation="GHSNQPVAPTFAVDAGAPPGEGAGSYPPIAEEPGAPGFTVPYVI
                     TSGQFNNTRLSEITSLEYTTYSSVAGYAPSLSLDVR"
     assembly_gap    3537..3551
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3552..4507
                     /locus_tag="PLV68_06330"
     CDS             <3552..4507
                     /locus_tag="PLV68_06330"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HRE00897.1"
                     /translation="AFTNQWQGRFVYVPNNPTAGWQTWDAMDDDLANGGWYASNQGAS
                     EGLCGLSALCTWSDLLTNWPGATITNPDQANAGALGQVAVRAGTTTEVGLIGGTFHVD
                     QIVIGRPAGPNYPTTTYDFEPELPGAPTGVLAVAGNGQVTLSWTAPAYTGGLAITGYV
                     VAGDGTCTPSPADATSCVVTGLTNGTEYSFTVAATNALGTGAASASASATPGAVPSLA
                     QNLTATAGDEQVVLSWDAPLDDGGSPVTGYVVTTTGGSCAVSGLTATCTGLTNGTEYS
                     FTVAATNSTGTGSATAPVTASSPTCRRSPPRRASPWPNRSP"
     gene            4486..>5285
                     /locus_tag="PLV68_06335"
     CDS             4486..>5285
                     /locus_tag="PLV68_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00898.1"
                     /translation="MAESVTVTSGALNGWSWLTDGAVNDNQSVTPVFAVGGGVGVGSA
                     IYPTVPANDAIMINTAAFDGVRLADVTALSYSTTSTSATYAPTLGIEIAYSPSATTWQ
                     GRLVFVPTAPTNGAHTWNTLDPAAGRWYPSNAAGTLCVGGCTWAQVLANYPDASIDNP
                     ANAGADAGRLVIRAGDTSSNYSTETFTVDSVVIGRHDGAVTRTTTFDFEPTAPSAPTS
                     VVATAGDASASVSWTAPADDGGLAVTYTVVSSPTGGSCTVTGTTASCTG"
     assembly_gap    5286..5461
                     /estimated_length=176
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5473..>5689)
                     /locus_tag="PLV68_06340"
     CDS             complement(5473..>5689)
                     /locus_tag="PLV68_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00899.1"
                     /translation="GVALVSTAAPGTVRGVTGTAGDDAGPVPAALVAVTVTSYVVPLV
                     SPPSSHVVAPVVVHVISGPDGGVAVTV"
BASE COUNT          864 a         1936 c         1732 g          778 t
ORIGIN      
        1 gccagctcgg tgatctggtc cacctggccg acggtccgaa caagctgcag aacgtgacga
       61 tccagttcga cgcgtgggcc tgccagcgtg gcgaccgcac cacctgcgac accgatccgg
      121 gtgcgacctt cgcccacccg atcaccgtca ccatctactc cgccgacctc tccaccaagc
      181 tggccgaggt gaccgagatc gtcgatgtgc ccttccgccc gacgcgcgat gccacctgcc
      241 ccgctctgtc cagtgagccg gccggctcgg gatggcgggc cgacgacggc gagtgctaca
      301 acggctacgg cttccgctgg acgttcgact tcccgtcggg cccggtgctg ccgtccgacg
      361 tggtgtggac ggtgannnnn nnnnnnnnnn acccgtccgt gtactccaac ccagtggcgg
      421 gtgtgaccgg cgtgagcggc ccgggggaca gcctcaacgt gtcgatctag tcgcgtcagc
      481 aactcgcctt cgcgggcacc gacaccgacc cgaggacgtt ctacctcgac agcagcaacc
      541 aggcgtacta ctgcgacacc gcaccgaagg acgtgctgcg tcccacgtat atctgctggg
      601 gcggctatcg cccgatggcg accatccgca ccaccgggcc gatcacgcac gagtcggaga
      661 cggtcaccgt caccagcgac gctttgcacg gctggggttg gctgaccgac ggcaacaatc
      721 ccaacgccac ggtgacgccg cgcttcacca ccgcgaccga cacgtcgggt gaggacggtt
      781 ccggttcgta cccggcgctg caggccaacg ttccgatcat cgtcgcgacc aacgactacg
      841 acggcgtgaa gctggccgac ctgaccgcgc tgtcgtacga cacatactcc agccgggtcg
      901 actacgcgcc gagcctggcg ctggacgtgc ggctgaacag ctccaacgtg tggcagggac
      961 gcttggtgtt catcgccagc ggcgacacca gcggatggaa gacgtgggac acgttcgaac
     1021 ccaccgcggg actctggtac gccagcaacg ccaccgcgtc cggtggtaac tgccgcgaca
     1081 cgctgtgcac ctgggacgag gtgttgacca actggcccga cgccatgatc gacaacccgg
     1141 acggcagcag ccccggccgc ctcagcgtgc gggccggaac gaccgacccc gactacagcg
     1201 cggcgacgtt ccacgtcgac cacatcgtgg ttggcgcgat gtccgctggg ccgggcacgc
     1261 tgaccaacac ggtcaccacg ttcgacttcg aaccacagtt gccaacggtg ccgcggttcg
     1321 agaacgccac accgggcgat ggccgggcgc tgctgaagtg gacccgtccg gtcagcgacg
     1381 gcggcctgcc gatcaccggc tatgtcgtga ccacgcctga cggcggcacc tgcaccgtca
     1441 gcggcacatc ggcgctgtgc accggtctca ccaacggctc gccgtacggc ttcaccgtcg
     1501 ccgcggtcaa cgacttgggc accggcccgg ccgtcgcgat cggtccggtc acaccggccg
     1561 agcacgcggt gcaggtcgtc gcctacgact cgatcggcct caacccgcag ggcaactacg
     1621 ccagcctcgg ctaccaggcc acgcaaacca gcgagttcgg cgacctggtc gagttcgcac
     1681 ccggcccgcg cacgctggac gacatcaccg tccagttctc gtcgtgggcc tgcgaaaccg
     1741 gttccggcac gtcgtgtctg accacgcctg ggtccacgtt cgaccacccg atcaccgtga
     1801 ccgtgtacgc ggtcgacgag tccggcccca ccccggtcgt gggtgcggtg ctggcgacga
     1861 tgaccaagaa cgtcgccgtg ccgtaccgtc cgtcggccaa cgccacctgt gcaggcggca
     1921 ccacgtggaa ggccgccaac ggcacttgct acaacggctt cgccttcacc gagcggttcg
     1981 acttccccga gggcatcacc gtgcccgacc gcgtgatctg ggccgtcagc tacaacacgc
     2041 agacgtgggg ctacgacccc atcggtctgc ccggcccgta caccagcctc aacgtgggcg
     2101 ccgatagccg tggcagcgcg accgcgttcg tcggcaccga cgtcaacacc gacacggtgt
     2161 tctgggccac caggcacgcc ccgttctatt gcgacgatgg cgcgggtggc accaacacgc
     2221 tgcgcgagga ctgtgactgg acgggcaacc gcccgatggc cacgatccgc acccagggcg
     2281 gcgccaccga caccacggtc gagctgaccg tcacccgcga tgcgctccag ggttgggaat
     2341 ggctcaccga cggcaacaac cccaacgtgg cggtccccgc ggtgttcacc gccgacacca
     2401 acacgtccgg cgagttgggc tcgggttcgt acccgacgct ggccccgaac gagccggtca
     2461 tcgtgtcgac caacgcctac gacggcgtga agctggccga catcagcgag ctcggctacg
     2521 agacctactc cagccgcagc gactacgcgc cgggcctgtc gctcgacgtg aagctgacgc
     2581 ccagcgccac gacctggcag ggccgcctcg tgttcgtgcc gtcgggaacg accaacggtt
     2641 ggcagacctg ggacaccctc gatgccacca acggcttgtg gtacgccagc aaccagacgg
     2701 ccgcggccgc cgccggccga tgcggcctga gcgcgctgtg cacctgggat caggtgctca
     2761 cctactggcc gcaggcccag gtgggcaacc ccgacaccaa caacaacccg ctggctcccg
     2821 gccggttgtc ggtgcgggcg ggcattaccg ctaccgacta cagcgccgac acgttccacg
     2881 tcgaccacgt ggtggtcggt gtggtcgcga ccggtgtgtc gccggtgacc aacacgatca
     2941 cgacctacga cttcgagccg tcgctgcctg cagccccgac cgcggtcatc gcggtagcgg
     3001 gcgacacgac cgcaacggtc agctggaccg ctcccgactc gggcggcacc ccgctgcagt
     3061 cgtacacggt caccagcgcg cccggcgcca agacctgcac cgcgacggca cccgcgacga
     3121 cgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnggcca
     3301 ttcgaaccag ccggtcgcgc cgacgttcgc cgtcgacgcg ggagcgccac cgggtgaggg
     3361 tgcgggttcg tatccgccga tcgccgaaga gccgggcgcg cctggcttca cggtgccata
     3421 cgtgatcacc agcgggcagt tcaacaacac ccgcttgtcg gaaatcacct cgttggagta
     3481 cacgacgtat tcgtcggtgg cggggtatgc cccgtcgttg tcgctcgacg tgcgctnnnn
     3541 nnnnnnnnnn ncggccttca ccaaccagtg gcagggccgg ttcgtgtacg tgccgaacaa
     3601 tccgaccgcc ggttggcaga cgtgggatgc gatggatgac gatctcgcca acggcggctg
     3661 gtacgccagc aaccagggcg cgtcggaggg cctgtgcggc ctgtcggcgc tgtgcacctg
     3721 gagcgatctg ctcaccaact ggccgggtgc gactatcacc aaccccgacc aggccaacgc
     3781 cggtgcgctc ggtcaggtgg ccgtgcgggc cgggaccacg accgaggtcg gtctgatcgg
     3841 tggcacgttc cacgtcgacc agatcgtgat tggtcgtccc gctgggccga actacccgac
     3901 gacgacctac gacttcgagc ccgaactgcc cggcgcgccg actggcgtgt tggctgtggc
     3961 cggcaacggc caggtcacgt tgagctggac ggcacccgcc tacacgggcg gtctggcgat
     4021 caccggctac gtcgtcgctg gcgacggcac ctgcaccccg tcgcccgctg acgcgacgag
     4081 ctgtgtcgtc accggtctga ccaacggcac cgagtactcc ttcaccgtcg ccgcgaccaa
     4141 cgcccttggc accggtgccg cctcggcgtc ggcgtcggca acgcctggcg cggtgccgag
     4201 cctggcgcag aacctgaccg ccaccgccgg tgacgaacag gtcgtgctga gctgggacgc
     4261 gccgctcgac gatggcgggt cgccggtgac cggctacgtc gtgaccacca cgggtggaag
     4321 ctgcgccgta tccggcctga ccgccacctg taccggtctg accaacggca cggagtactc
     4381 cttcaccgtc gctgcgacca acagcaccgg taccggctcg gcgaccgcgc cggtcaccgc
     4441 gagttccccg acctgccgcc ggtcaccgcc acgccgcgcc agcccgtggc cgaatcggtc
     4501 accgtgacgt cgggcgcgtt gaacggttgg tcgtggctga ccgatggtgc ggtcaacgac
     4561 aaccagagcg tgacgccggt gttcgccgtt ggtggcggtg tgggtgtcgg ctcggccatt
     4621 tacccgacgg tgcccgccaa cgacgcgatc atgatcaaca ccgctgcctt tgatggtgtg
     4681 cgcttggccg acgtgacggc gttgtcgtac tcgaccacgt cgacgagcgc gacctacgcg
     4741 ccgacgctgg gcatcgagat cgcctactcg ccgtcggcca ccacctggca gggccggttg
     4801 gtgttcgtgc cgaccgcgcc caccaacggt gcgcacacct ggaacacgct cgacccggcc
     4861 gctggccgtt ggtacccgag caacgccgcg gggacactgt gcgtgggtgg gtgcacctgg
     4921 gcacaggtgc tggccaacta tccggacgcg tcgatcgaca acccggccaa cgccggtgcc
     4981 gacgccggac ggttggtgat ccgcgccggt gacacctcgt cgaactacag caccgagacg
     5041 ttcaccgtcg acagcgtggt gatcggtcgt cacgacggtg cggtcacccg cacgaccacg
     5101 ttcgatttcg agccgaccgc tccgtcggcg ccgacgtcgg ttgtggccac ggctggcgat
     5161 gcgtcggctt ctgtgtcgtg gacagcgccc gccgacgacg gtggcctggc ggtgacatac
     5221 accgtcgtgt cgtcgcccac tggtgggtcg tgcacggtca ccggcacgac ggcatcgtgc
     5281 acgggnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5461 nccgatcacg ggctacacgg tgacggcgac gccgccgtcg ggcccggaga tcacgtgcac
     5521 caccaccggt gcgaccacgt gtgagctggg cgggctgacc aacggcacca cgtacgacgt
     5581 cacggtgacg gccaccaacg cggccggaac cgggccggcg tcgtctccgg ccgtgccggt
     5641 caccccgcgc acggtgcccg gcgctgcggt gctcaccagc gcgaccccc
//
LOCUS       DAORNF010000123         5687 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_10503_length_5687_cov_1.528764, whole genome shotgun sequence.
ACCESSION   DAORNF010000123 DAORNF010000000
VERSION     DAORNF010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 5687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5687
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10503_length_5687_cov_1.528764"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /locus_tag="PLV68_06345"
     CDS             <1..608
                     /locus_tag="PLV68_06345"
                     /inference="COORDINATES: protein motif:HMM:NF013259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfite reductase"
                     /protein_id="HRE00900.1"
                     /translation="VEPIYGDTYLPRKFKIGVAWPGDNSIDIFSQDVGLLPTLADGTS
                     GELTGFVVYVGGGMGMNWSREDDTYPRLASVLGWVPAEHVVDVVEAVVTTQRDHGDRV
                     DRQQARLKYLVDKRGTEWMIEQVQTRSGVTLQPVPDLPPFAAADYHGWLAEGDQGTWT
                     LGLPVQSGRVADADSVALRSALRELAGSGMVAGFHVSPVKT"
     gene            608..1159
                     /locus_tag="PLV68_06350"
     CDS             608..1159
                     /locus_tag="PLV68_06350"
                     /inference="COORDINATES: protein motif:HMM:NF013259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00901.1"
                     /translation="MISGIAPGDRAQVEAVLRRHEVALADDVSALTRLAIACPALPTC
                     GQALGEAERVLPQLVESLDKSLATGGAQGAAIRLNMTGCPNGCARPYNAEIGIVGRTK
                     TNYDVYVGGTVGGGRMAERLRADVALADIPALLEPVFAEFANNAREGEGFGDFCARTG
                     IDALTTVLPAPVVKRRRSAETSV"
     gene            1162..>1614
                     /gene="cobA"
                     /locus_tag="PLV68_06355"
     CDS             1162..>1614
                     /gene="cobA"
                     /locus_tag="PLV68_06355"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HRE00902.1"
                     /translation="MFGHGPDAHEPDVVPGSIALVGAGPGDPDLLTLRAARLLANADV
                     VVHDALVGEGVLALIPAHAERIDVGKRPGRGVAQELTNVLLVGLVRQGKRVVRLKGGD
                     PFVFGRGGEEATALAEAGITCEVVPGITSAVAAPAARVFPTSPSSSVRS"
     assembly_gap    1615..1704
                     /estimated_length=90
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1705..1937
                     /locus_tag="PLV68_06360"
     CDS             <1705..1937
                     /locus_tag="PLV68_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HRE00903.1"
                     /translation="GVSQRAAIAAALMDGGLAGETPVAAIAQATTTEQVVGRCRLVDL
                     ALLDVESPATLVIGAVAALDVTAPVAAVATTA"
     assembly_gap    2036..2196
                     /estimated_length=161
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2197..2791
                     /locus_tag="PLV68_06365"
     CDS             <2197..2791
                     /locus_tag="PLV68_06365"
                     /inference="COORDINATES: protein motif:HMM:NF017932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M90 family metallopeptidase"
                     /protein_id="HRE00904.1"
                     /translation="AAQACLLVLELGDDDHDPLDFYGAVSTVIVHPKPVVLQGQRSTA
                     TSGVMTDAPTRIDGQAHYEGPVIVAWNAVTTDARHPRRGLNVVYHEFAHKLDMLDGTV
                     DGTPPPPLLGLADRPRWIEVCTREFRSIRAGTDDGFLREYGGTDPGEFFAVAVEMFFS
                     RPVEMRDHRSELYELLTGVFRQDPARRYPEPASAPGA"
     gene            complement(2794..3141)
                     /locus_tag="PLV68_06370"
     CDS             complement(2794..3141)
                     /locus_tag="PLV68_06370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00905.1"
                     /translation="MGQPRCLPTLIVCTGKDCRDEKGYRSLLDLARDEHGSLAAPCQG
                     LCDGPVVAVRYGDDVRWYRRARSKSARKLLAKLLHSSHPGTGKAAQGLREFEVRKRHN
                     IIRGSNRLKQLNA"
     assembly_gap    3266..3372
                     /estimated_length=107
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3373..3618)
                     /locus_tag="PLV68_06375"
     CDS             complement(<3373..3618)
                     /locus_tag="PLV68_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HRE00906.1"
                     /translation="MSATPAHRDLDHDRVFTVLNSILEAELSGVVRYTHYSLMIIGPN
                     RIPLVAFMKAQATESLLHAQQVGEILTGLGGHPSLRIT"
     assembly_gap    3711..3745
                     /estimated_length=35
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3746..4930)
                     /locus_tag="PLV68_06380"
     CDS             complement(<3746..4930)
                     /locus_tag="PLV68_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRE00907.1"
                     /translation="MDFEIPAHIQETLDQLDDFIEREIKPLQAQDDNERFFDHRREFS
                     RTDIEAGGVPTQDWEDLLAEMRRRADAAGWLRLALPEEFGGKAADNLTMAVIREHLAA
                     KGLGLHNDLQNESSVVGNFPTVLMMRDFGTPEQQASWMPGFLDGTRRLAFGLTEPNHG
                     SDATYLETTAVRDGDEWVLNGMKRWNSGLHHATHDIIFARSSGEPGDPVGITAFLVPT
                     DAPGFNVDFFWWTFNMPTDHAEVSMTDVRVGDDAVFGRVGHGLELAQHFVHENRIRQA
                     ASSLGAAQYCINEAVAYANSRVTWGKKLSANQGIQFPLVELHTEAAMLRQLIRYTAWM
                     LDRKHHMEVTHLVAMCNYRANRLVCEAADRAMQTCGGVGYSRHMPFEHIYRHHRRYRI
                     TEG"
     gene            complement(5169..>5389)
                     /locus_tag="PLV68_06385"
     CDS             complement(5169..>5389)
                     /locus_tag="PLV68_06385"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRE00908.1"
                     /translation="VEGHDVELPSGALHDASEMAREVPTVMIFSSSIDGLSHTKEEDT
                     PEAHLEMAADAFYRTCRRAIDLVATHQL"
     assembly_gap    5390..5417
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5418..>5687)
                     /locus_tag="PLV68_06390"
     CDS             complement(<5418..>5687)
                     /locus_tag="PLV68_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00909.1"
                     /translation="LVAAARFAIALKEIGARHDGVATAGHVACWPGVVTAVPGETRMT
                     MDMRHIDAGELAAMFAEMQQAAHEAAEAEGCTVEFEHLFRIPPMP"
BASE COUNT          782 a         1766 c         1845 g          873 t
ORIGIN      
        1 gcgtcgagcc gatctacggc gacacctacc taccgcgcaa gttcaagatc ggggtcgcct
       61 ggccgggcga caacagcatc gacatcttca gccaggacgt cggcctgctg cccaccctgg
      121 ccgacgggac ctcgggcgag ctgaccggct tcgtcgtcta cgtcgggggc ggcatgggca
      181 tgaactggtc gcgtgaagac gacacgtacc cgcgcctggc gagtgtgctc gggtgggtgc
      241 cggccgagca cgtggtcgac gtggtcgagg ccgtggtcac cacccagcgc gatcacggcg
      301 accgcgtcga tcgccagcag gcccggttga agtacctggt cgacaagcgc ggcaccgaat
      361 ggatgatcga gcaggtccag acccgcagcg gcgtcacgct gcagcccgtg cccgacctgc
      421 cgccgttcgc cgccgccgac taccacggtt ggctggccga aggtgaccag ggcacctgga
      481 ccctcgggct gccggtgcaa tcggggcgcg tcgccgacgc tgactcggtg gcgctgcgca
      541 gcgcactgcg cgagttggct gggtccggga tggtcgccgg gttccacgtc tcgcccgtca
      601 agacctgatg atcagcggca tcgcccctgg cgaccgggcg caggtcgagg cggtgctgcg
      661 ccgccacgaa gtcgcgctcg cggacgacgt gtctgctctg acccgcttgg ccatcgcctg
      721 cccggctctg cccacctgcg gccaggcgtt gggcgaggcc gagcgggtgt tgccgcagct
      781 cgtcgagtcg ctcgacaagt cgctggcgac cggcggtgca cagggcgcgg cgatccggct
      841 caacatgacc ggctgcccca acggttgcgc ccgcccgtac aacgcggaga tcggcatcgt
      901 cggacgcacc aagacgaact acgacgtgta cgtcggtggc acggtcggtg gcggccggat
      961 ggccgagcgg ttgcgggccg acgtcgccct ggcggacatc ccagcactgc tcgagccggt
     1021 gttcgccgag ttcgccaaca acgcgcgcga gggtgagggc ttcggcgact tctgcgcccg
     1081 caccggcatc gacgcgttga ccaccgtgtt gcccgctccc gtcgtcaaac gccgccgcag
     1141 cgccgagacg tcggtatgag cgtgttcggt cacgggccag acgcccacga accagatgtc
     1201 gtaccgggca gcatcgcctt ggtcggtgcg ggccccggcg atcccgacct gttgacgctg
     1261 cgcgcggctc gcctgttggc gaacgccgac gtggtggtgc acgacgcttt ggtgggcgag
     1321 ggagtgctcg cgctgatacc cgcccacgcc gagcggatcg acgtcggcaa gcggcccggg
     1381 cgcggtgtgg cccaggagct gaccaacgtg ttgctggtcg ggctcgtccg ccagggcaag
     1441 cgggtggttc gcctgaaggg aggcgatccg ttcgtgttcg gccgcggcgg ggaagaggcc
     1501 acggcgctgg ccgaggcagg catcacctgc gaggtcgtgc caggcatcac ctccgcggtg
     1561 gccgcgccgg cggcgcgagt tttcccgacg tctccaagtt cttcggtccg cagtnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnntgggcg tatcgcagcg tgccgccatc gctgccgcgc
     1741 tgatggacgg cggcctcgca ggcgagaccc cggtcgccgc gatcgcccag gcaaccacca
     1801 ccgagcaagt cgtcggccgc tgccgcctcg tcgatctggc cctgctcgac gtcgagagcc
     1861 ccgcgaccct ggtgatcggc gccgtcgccg ccctcgacgt caccgcgcct gtcgccgccg
     1921 ttgccaccac ggcctgaccc gcgggtgggg gatgggggtt cgtgggtatc gtcggctctg
     1981 tggtgttcgg gttcggcagg cggcggcgcg acgcggcgtt gggtcagccg ttcccnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncgcc gcccaggcgt gcttgttggt
     2221 gctcgaactc ggcgacgacg accacgaccc gctcgacttc tacggcgcgg tctcgaccgt
     2281 gatcgtccac cccaagccgg tcgtcctgca gggccagcgt tcgacggcga cgagcggcgt
     2341 gatgaccgac gcgccgaccc gcatcgacgg ccaggcgcac tacgaaggcc cggtgatcgt
     2401 ggcctggaac gcagtcacga ccgacgcccg tcacccgcgc cgcggactca acgtcgtcta
     2461 ccacgagttc gcccacaagc tcgacatgct cgacggcacg gtcgacggca caccgccccc
     2521 gccactgctc ggcctggcgg acaggccgcg gtggatcgag gtctgcaccc gcgaattccg
     2581 atcgatccgt gctgggaccg acgatgggtt cctgcgcgag tacggtggca ccgatccggg
     2641 tgagttcttc gcggtcgcgg tcgagatgtt cttctcccgc ccggtcgaga tgcgtgacca
     2701 ccgcagcgag ctgtacgaac tgctcaccgg tgtcttccgt caagatcctg cccgccgcta
     2761 tcccgagccg gcgtcggccc cgggggcctg acgttacgcg ttgagctgct tgagacggtt
     2821 cgagccgcgg atgatgttgt gccgcttgcg gacttcgaac tcacggaggc cttgtgcggc
     2881 cttgccggtg ccagggtgcg acgagtgcag cagcttggcc agcagcttgc gagccgactt
     2941 cgaccgggcc cggcggtacc aacgcacgtc gtcgccgtag cgcacggcga cgaccgggcc
     3001 gtcgcacagg ccttggcacg gggccgccag cgacccgtgc tcgtcgcggg ccaggtcgag
     3061 caacgatcga taacccttct cgtcgcggca gtccttgccg gtgcagacga tcagcgtggg
     3121 gagacagcgt ggctggccca tgggtcgctc attcggagat ggccgcgaag tcgcgcagca
     3181 tcttgttgag ctctaactga tgttgttctt cggcgctgat ctggccgcgg gcgaactctt
     3241 cgaggaacac cgagctgtcc tgcacnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nncgtgatgc gcagagaagg gtgaccgccc aggccggtca ggatctcgcc
     3421 gacctgttgc gcgtgcagca gcgactcggt ggcctgagcc ttcatgaacg cgaccagcgg
     3481 gatgcggttg gggccgatga tcatcagcga gtagtgcgtg tagcgcacca cgccggacag
     3541 ttctgcttcg aggatcgagt tgagcacggt gaacacccgg tcgtgatcga ggtcgcgatg
     3601 ggcaggggtg gcggacacgg ggtctcctcg gcagtgaggc ttgttcggct accctaacgg
     3661 ccggacagat ggacgcagca actcggtgga ctacttgcgg ccgaagccga nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnngccct cggtgatgcg gtagcggcgg tgatggcggt
     3781 agatgtgctc gaacggcatg tggcgcgagt agccgacacc gccgcaggtc tgcatcgccc
     3841 gatcggccgc ttcgcacacc agccggttgg cgcggtagtt gcacatcgcc accagatggg
     3901 tcacctccat gtggtgcttg cggtcgagca tccaggccgt gtaacggatg agctggcgca
     3961 gcatcgccgc ctcggtgtgc agctcgacca gcgggaactg gatgccctgg ttggcgctga
     4021 gcttcttacc ccacgtgacc cggctgttgg cataggcgac cgcttcgttg atgcagtact
     4081 gcgctgcgcc gagcgacgat gcggcctggc ggatgcggtt ctcgtgcacg aagtgctggg
     4141 ccagctcgag cccgtggccg acccggccga acaccgcgtc gtcgccgacg cgcacgtcgg
     4201 tcatgctgac ctcggcgtgg tcggtgggca tgttgaacgt ccaccagaag aagtcgacgt
     4261 tgaagccggg tgcgtcggtg ggcaccagga acgcggtgat gcccaccgga tcgccgggct
     4321 cgccgctgct gcgggcgaag atgatgtcgt gggtggcgtg gtgcagcccg ctgttccagc
     4381 gcttcatgcc gttgagcacc cactcgtcgc catcgcgcac cgcggtggtc tccaggtatg
     4441 tggcgtcgct gccgtggttg ggttcggtca ggccgaacgc caaccgccgg gtgccgtcga
     4501 ggaagcccgg catccacgac gcctgctgct cgggtgtgcc gaaatcgcgc atcatcaaca
     4561 cggtggggaa gttgccgacg acgctcgact cgttctgcag gtcgttgtgc agcccgagcc
     4621 ccttggcggc gaggtgctcg cggatgacgg ccatcgtcag gttgtcggcc gccttaccgc
     4681 cgaactcttc cggcagcgcg agacgcagcc agcccgcagc gtcggcccgg cgacgcatct
     4741 cggccagcag gtcttcccag tcctgggtgg gcacaccgcc cgcctcgatg tcggtgcgtg
     4801 agaactcgcg gcggtggtcg aagaatcgct cgttgtcgtc ctgagcctgc aacggcttga
     4861 tctcgcgctc gatgaagtcg tcgagttgat cgagggtctc ttggatgtgc gccggaatct
     4921 cgaagtccat gtaccgacag tacgggcagc gccccacccc atcggcatcg ggtcagtaga
     4981 acgtgccgca gaaggggctc gtcgaccgca tcgccgacca cccgcttgac gaagtagtgg
     5041 tctcgcgaca ccgacaggac ccgccatcgg ccgacacggt tgtacagatc gggaaccacg
     5101 tccatcacgt cgtccggggt caacagccgc gtcgagcccg tgatcgggcc atcatcccga
     5161 ccggaggttc agagttggtg ggtggcgacg aggtcgatgg cgcggcggca ggtgcggtag
     5221 aaggcgtcgg ccgccatctc caggtgagcc tcgggcgtgt cctcttcctt ggtgtggctg
     5281 agtccgtcga tcgacgagga gaagatcatc accgtcggca cctcgcgcgc catctcgctt
     5341 gcgtcgtgca acgcgccgct gggcagttcg acgtcgtgcc cttcgacctn nnnnnnnnnn
     5401 nnnnnnnnnn nnnnnnnaac ggcatcggcg ggatacggaa caggtgctcg aactcgacgg
     5461 tgcagccttc cgcctcggca gcttcatggg cagcctgctg catctcggcg aacatcgccg
     5521 ccaactcgcc cgcgtcgatg tggcgcatgt ccatcgtcat ccgcgtctcg cctggcaccg
     5581 cagtgaccac acccggccaa caggcgacgt gaccggcggt ggcaacaccg tcgtggcggg
     5641 cgccgatctc cttgagcgcg atcgcgaacc gggccgcggc gaccagc
//
LOCUS       DAORNF010000124         5660 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_10567_length_5660_cov_1.838180, whole genome shotgun sequence.
ACCESSION   DAORNF010000124 DAORNF010000000
VERSION     DAORNF010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 5660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5660
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10567_length_5660_cov_1.838180"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            19..>357
                     /locus_tag="PLV68_06395"
     CDS             19..>357
                     /locus_tag="PLV68_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00910.1"
                     /translation="MSAYEAGAAGAAGATQDSTTAGDGVGTSPDVDPDTEMAKTTAGQ
                     QVVDQQRWDGAPVLTDIAAVRALVGSEQFAGWPGSPPQASTAPTTAAPATTVDQQNTG
                     DAPSTSAASTT"
     assembly_gap    358..458
                     /estimated_length=101
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            605..934
                     /locus_tag="PLV68_06400"
     CDS             605..934
                     /locus_tag="PLV68_06400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00911.1"
                     /translation="MPSNPLNDPTWAPKLADTVERVVGQVRDKTTKPLLVAYRGLVFG
                     LVAAFSGVFMVILVVITAIRSLQALIEIGTSHHDAVWISYLAIGALFSIAGLVLLKKR
                     FPSETAS"
     gene            931..1911
                     /gene="trxB"
                     /locus_tag="PLV68_06405"
     CDS             931..1911
                     /gene="trxB"
                     /locus_tag="PLV68_06405"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HRE00912.1"
                     /translation="MSHHPVIIIGSGPAGLTAAIYTARAQLAPLVIEGEPSSTSDQPG
                     GQLMLTTEVENFPGFPTGIMGPELMSNFREQAVRFGAQFVTEKVTAVDFSARPFKVWV
                     RDMLYEGDAVIVSTGARSLMLDLPDEQRLLGYGLSTCATCDGFFFRNHEIAVVGGGDS
                     AVEEATFLTKFATKVIMIHRRDTLRASKIMQDRALSNPKIEMRWNSTVTALHGTDRLE
                     GITITDVNTGEQTDLAVTGLFVAIGHRPNTDLFKGVLDMEDNGYLVTQPGSTYTNIDG
                     VFACGDVQDHTYRQAITAAGSGCMAAIDAERWLEATAHAAGHTSDTASAG"
     gene            2021..2359
                     /gene="trxA"
                     /locus_tag="PLV68_06410"
     CDS             2021..2359
                     /gene="trxA"
                     /locus_tag="PLV68_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HRE00913.1"
                     /translation="MADGIVTLTTSTFDETILGAETPVVVDFWAEWCGPCKMIAPILG
                     EIATEQAGKVTIAKLNVDENPDLAMKFNVMSIPTLLVFHRGEIVKRLVGAKGKGQLMQ
                     ELSEFLVTSP"
     assembly_gap    2601..2626
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2627..>3143
                     /locus_tag="PLV68_06415"
     CDS             <2627..>3143
                     /locus_tag="PLV68_06415"
                     /inference="COORDINATES: protein motif:HMM:NF013625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HRE00914.1"
                     /translation="GVLGFDCGKVDGIFGPLADAALRTFQADCGLLVDGVCGPVTLQV
                     LERVRRQTGTGPGVATMRERVTMRERTTSLQNMRVVIGHFGGMSIMTRALARDLRHHG
                     AAVISVDEPDARVQAAAANQFDADVYLGFQVEPVVHTTPVHGMPMPAAAPPPLSATQS
                     SAPQSSAGWSSA"
     assembly_gap    3144..3226
                     /estimated_length=83
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3574..4563)
                     /locus_tag="PLV68_06420"
     CDS             complement(3574..4563)
                     /locus_tag="PLV68_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HRE00915.1"
                     /translation="MARLGGLGKGLGALIPSDIDATQQLERPTIDDPGATTPMATDTA
                     AATAPRLAELPVDALSPNPNQPRVHFDEELLEELAASIREIGVLQPVLVRASGPGVYE
                     LIAGERRWRAARRAGLTTIPAIVRITGDLQSVEQALVENLHRQDLTPLEEAAAYQQLI
                     EDFNLTHEQVSSRVGKSRSAVSNTLRLLTLPPSIQHLLADGRLSAGHARALLGSPDRA
                     FQEYLAKRAVDDSLSVRAVEEAIRDRQAGGAGSPDDAVPPVTPTADGAGLTPVTRLRP
                     PGLLELEHLLAEHLDTKVAVSMGARRGRVSIEFADLEDLERIYRQMVEPTTRD"
     gene            complement(4538..5317)
                     /locus_tag="PLV68_06425"
     CDS             complement(4538..5317)
                     /locus_tag="PLV68_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRE00916.1"
                     /translation="MPRVIAVANQKGGVGKTTTTINVGACLAELGFRTLVIDLDPQGN
                     ASTGLGIDNRGLETSMYHVIMHDVPLENCIEPAAVKNLFVAPASLDLAGTEIELVPAF
                     SRENRLKRAIEGVIDDYDYVLIDCPPSLGLLTVNGLTAATEVMVPIQCEYYALEGLGQ
                     LLRNVDLVRRNLNPALTVSTIVCVMYDARTKLADQVVREVRDHFGDKVVRTVVPRTVR
                     LSEAPSFGQPIITFDGTSRGAVAYRLVAKEVSSHGEARRTG"
BASE COUNT          911 a         1887 c         1727 g          925 t
ORIGIN      
        1 gagtcggccg actccccgat gagcgcctac gaggccggcg ctgctggcgc ggccggcgca
       61 acgcaggact ccaccaccgc gggcgacgga gtcggcacca gccccgacgt cgacccagac
      121 acagagatgg ccaagacgac cgccggccaa caggttgtcg atcaacagcg ttgggatggt
      181 gcgcctgtgc tcaccgacat cgccgcggtg cgcgccctgg tcggcagcga gcagttcgcc
      241 ggttggcccg gctcgccgcc ccaggccagt accgctccca ccacggccgc accggccacc
      301 accgtcgacc agcagaacac gggtgacgct ccctcgacct cggcagcgtc cacgacgnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngg cgggcctggc ccacgtgttg
      481 accgcgaccg actgctcacc catcgtcgtc gtgcccctcg ctgctgggtg acgggttcgc
      541 gggcgttacg ccgcagcgtg acgcgccgcc ggatgacggg tcgcggcccc gttaggctcg
      601 ggccatgcca agcaatcccc tcaacgaccc gacctgggcg cccaagctgg ccgacaccgt
      661 cgagcgcgtg gtcggccagg ttcgcgacaa gacgaccaag ccgctgttgg tggcctatcg
      721 cggcctcgtg ttcggtctgg tcgcggcgtt ctccggcgtg ttcatggtga ttctggtggt
      781 catcaccgcc atccgctcgt tgcaggcgtt gatcgagatc ggcacctcgc atcacgacgc
      841 ggtgtggatc agctacctcg ccatcggcgc actgttcagc atcgctggtc tggtgctcct
      901 caagaagcgc tttccgtcag agaccgcatc gtgagccacc accctgtcat catcatcggg
      961 tcgggccccg ctggactgac cgcggccatc tataccgccc gcgcccaact cgctccgctc
     1021 gtgatcgaag gcgagcccag cagcaccagc gaccagcccg gcggtcagct gatgctcacc
     1081 accgaggtcg agaacttccc cgggttcccc acgggcatca tggggcccga gttgatgagc
     1141 aacttccgcg agcaggctgt gcgcttcggc gcccagttcg tcaccgaaaa ggtcaccgcg
     1201 gtcgacttct cggctcgccc cttcaaggtg tgggtgcgcg acatgctcta cgaaggtgac
     1261 gcggtcatcg tgtcgacggg cgcccgctcg ctcatgctcg acctgcccga cgagcagcgt
     1321 ttgttgggct acggcttgtc cacctgcgcc acctgcgacg ggttcttctt ccgcaaccac
     1381 gagatcgcgg tcgtcggtgg tggcgacagc gccgtcgaag aggccacgtt cctcaccaag
     1441 ttcgccacca aggtcatcat gatccaccgc cgcgacacgc tgcgggcgag caagatcatg
     1501 caagaccgcg ccctgtcgaa ccccaagatc gagatgcgct ggaactccac cgtcaccgcg
     1561 ctgcacggca ccgatcgtct cgaaggcatc accatcaccg atgtcaacac gggcgagcag
     1621 accgacctgg cggtcaccgg gctgttcgtc gccatcggcc accgccccaa caccgacctg
     1681 ttcaagggcg tgctcgacat ggaagacaac gggtacctcg tcacccaacc gggcagcacc
     1741 tacaccaaca tcgacggcgt cttcgcctgc ggcgacgtgc aagatcacac ctaccgccag
     1801 gcgatcaccg ctgccggctc aggctgcatg gccgccatcg acgccgaacg gtggttggag
     1861 gccaccgctc acgccgccgg ccacacctct gacaccgcgt cggcgggctg agacccacca
     1921 cgaacgggta gagacctgtt cacccggcgc ggggaatgcc gccaccgagg ccggtgttga
     1981 cctcgctagc atgacgctcc actcgaaagg aagccatccg atggccgacg gaattgtcac
     2041 cctcaccacc agcaccttcg acgagacgat cctgggcgcc gagacgcccg tcgtggtcga
     2101 cttctgggcc gagtggtgtg gaccctgcaa gatgatcgcc ccgatcctgg gcgagatcgc
     2161 caccgaacaa gccggcaagg tcaccatcgc caagctcaac gtcgacgaga accccgacct
     2221 ggcgatgaag ttcaacgtga tgagcattcc cactctgctc gtgttccacc gcggcgagat
     2281 cgtcaagcgc ctcgtcggcg ccaagggcaa gggccaactg atgcaggagc tcagcgaatt
     2341 cctggtcact tccccctgat ccctggccag cgcggcgagc cgatccgcga tctccagcgc
     2401 cgactcgccg ctgctggctt tctcgcgtcc ggggccgagc ccggggtgta ctgcgacggc
     2461 accgtcgccg cggtctgcgc gttccaacag gcggagccga agccccatcc ggagggactg
     2521 cttccctccc ggccgttggt gctctggccc tacaccgaga tggcggaccc gcgttggacg
     2581 tggggcaatc gcgtgattcg nnnnnnnnnn nnnnnnnnnn nnnnnncggg gtgctcggat
     2641 tcgactgcgg caaggtcgac ggcatcttcg gtccactcgc cgacgcggcc ttgcgcacct
     2701 ttcaggccga ctgcgggctg ctggtcgacg gggtctgtgg cccggtgacg ctgcaagtgc
     2761 tcgagcgcgt ccggcgccag accggcaccg gtccaggtgt ggccacgatg cgagagcggg
     2821 tcacgatgcg cgagcggacg acgtcgctgc agaacatgcg cgtcgtgatc ggtcactttg
     2881 gcggcatgag catcatgacc agggcgttgg ctcgcgactt acgtcaccac ggcgccgcgg
     2941 tgatctcggt cgacgagccc gacgcgcggg tgcaggccgc ggccgccaac cagttcgatg
     3001 ccgacgtcta cctgggcttt caggtcgaac cggtcgtgca caccacccct gtgcacggca
     3061 tgccgatgcc agccgcggca ccaccaccgc tgtccgcgac ccaatcgtca gccccccaat
     3121 cgtcagcggg ctggtcgtcc gcgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngatc cggctgctgc
     3241 cggccgtgcg cggcatgcgc ctgcaggtgc tgcgcgagac gaggatgccg gcactgctgg
     3301 tgacggtcac ccatgtccgc cgtgtgctcg accgctcgct ggcgattgcc agcatcgtgc
     3361 gcgcatcact cgagggttgg gtcgaacacc cgctctctgc ctgaacgcct cggttgctgc
     3421 ggtcatgtcg tgcggatccc tcgccttgcg tggtgacggg gcgacagaca gtgattcatc
     3481 cacagttcga ggtcgcgcct cccgtggaga tcgagtgttt atccacagag gtttccacac
     3541 gatgtgcgta aacctcatcc gcgggatgag tgctcagtcg cgcgtcgtgg gctcgaccat
     3601 ctggcgatag atgcgttcga ggtcttccag atcggcaaat tcgatgctga cgcgcccacg
     3661 tcgagcgccc atcgacacgg caaccttggt gtcgagatgc tctgcgagca ggtgttccag
     3721 ctccaacaga cccggtggac gcaaccgggt gaccggggtg agcccagcgc catctgcggt
     3781 gggtgtgacg ggcggcacag catcgtcagg gctccccgct cccccagcct gacggtcgcg
     3841 gatcgcttcc tcgacggcac gcaccgagag cgaatcatcc acagcccgtt tggccaagta
     3901 ctcctgaaaa gcccgatcgg gactgcccaa cagggcgcgg gcatgaccgg ctgacaggcg
     3961 accgtcggcc aacaggtgct gaatcgacgg gggcagggtc aacaggcgca aggtgttgga
     4021 gactgcggaa cggctcttgc cgacgcggga cgacacctgc tcgtgggtca ggttgaagtc
     4081 ctcgatcagc tgctgatagg cggctgcctc ttccagcggt gtgaggtcct gacggtgcaa
     4141 gttctcgacc agcgcctgct cgaccgactg caggtcgcca gtgatgcgca cgatggccgg
     4201 aatcgtcgtg agcccggcgc gacgtgccgc gcgccagcgc cgctcccccg cgatgagctc
     4261 gtacaccccc gggccagacg ctcggacgag cactggctgc agcacaccga tctcgcggat
     4321 cgacgccgca agttcctcga gcagctcctc atcgaagtgc acccgcggtt ggttggggtt
     4381 gggtgacagc gcgtccaccg gcagttccgc caaacgcggc gcagtcgcgg cagcggtatc
     4441 ggttgccatc ggtgtggtcg ctccaggatc gtcgatcgta ggacgctcca gctgctgggt
     4501 cgcgtcgatg tcggacggga tcaaagctcc cagtccctta cccagtcctc cgagcctcgc
     4561 catgcgagct cacctccttt gccaccaacc gatatgccac cgcaccccgg gacgtaccgt
     4621 cgaacgtgat gatcggctgt ccgaagctcg gcgcttctga cagacgcacc gtgcgcggca
     4681 cgaccgtgcg cacgaccttg tcgccaaagt ggtcgcgcac ctcgcgcacc acctggtcag
     4741 ccagcttggt acgagcgtcg tacatcacgc acacgatcgt gctgaccgtc agcgccgggt
     4801 tgagattgcg gcggacgagg tcgacgttgc gcagcagttg acccaggcct tcgagggcgt
     4861 agtactcgca ctgaatgggg accatcacct cggtggcagc cgtcaacccg ttgacggtca
     4921 acaagcccaa cgacggtggg cagtcgatca gcacgtagtc gtagtcgtcg atcacgccct
     4981 cgatcgcccg cttgagacga ttctcgcgcg agaacgccgg gaccagttcg atctccgtgc
     5041 cggccaagtc gagtgacgcc ggggcaacga acaggttctt gaccgcagcc ggttcgatgc
     5101 agttctccag cggcacgtcg tgcatgatca cgtgatacat cgacgtttcc aggccacggt
     5161 tgtcgatgcc gagaccggtc gatgcattgc cctgcgggtc gagatcgatc accagcgtcc
     5221 ggaagccgag ctctgccagg catgcgccga cgttgatcgt ggtggtggtc ttgccgacgc
     5281 cacccttctg gttggcgacc gcaatcacgc gcggcagcgg gtacgtggag cggggttcgg
     5341 gcgccgcctc ggttgcgtcg accgcgcctg aatctgctgg tgacgcagcg atggacgctg
     5401 attcagagga catagttcga cggatctccc tggtcagtcc gtggatcaca ccacgtcagt
     5461 agccgagtgt cagtagccga gtgtcgtgag ccgagttccc tccgcatgcg cgctgtcgaa
     5521 gacgcaacag ctcggtcgtt tgcaccatac tcccccaccc gccccaaccc gcgcaccctc
     5581 cgatgttttc tcgcaggccc ggttcgatgg tgtcccggct cgtggtgtag gagctcgtgt
     5641 tcttcccgat tgctgttgtc
//
LOCUS       DAORNF010000125         5649 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_10599_length_5649_cov_1.543261, whole genome shotgun sequence.
ACCESSION   DAORNF010000125 DAORNF010000000
VERSION     DAORNF010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 5649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5649
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10599_length_5649_cov_1.543261"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>251)
                     /locus_tag="PLV68_06430"
     CDS             complement(<1..>251)
                     /locus_tag="PLV68_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRE00917.1"
                     /translation="PIGGVVISDEIADTFKDRQFPGGLTYSGHVLACASVVASINIFK
                     EEGIVEHARRIGTDIIGPALSAMQDKHPSVGDVRGLGAF"
     assembly_gap    252..326
                     /estimated_length=75
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<327..1091)
                     /locus_tag="PLV68_06435"
     CDS             complement(<327..1091)
                     /locus_tag="PLV68_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRE00918.1"
                     /translation="MTAVDAPPSSTYANDRKHVFHSWSSQGALNPMVVTGGEGSWFWT
                     EDGTRYLDFSSQLVNVNIGHQHPKLVAAIQEQAAKLCTIAPGHAVEARSEAARLIAEL
                     APEGLNKVFFTNGGADATDNAIRLARLHTGRQKVLSTYRSYHGATGGAIAVTGDPRRW
                     PNETGVSGVIHIWGPYPYRSPFYASTPEEETERALAHLRSTIMVEGGHTIACIILETV
                     VGTNGILVPPPGYLQGVREICDEFGIVYIADEVMAGF"
     gene            complement(1185..>1438)
                     /locus_tag="PLV68_06440"
     CDS             complement(1185..>1438)
                     /locus_tag="PLV68_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HRE00919.1"
                     /translation="VQGVEMGFDGKTLIHPGQVDIANEVWAPSEDEVEHARRVIDAFE
                     EGLREGRGVITVDNRMIENLHVDNARRTLATAEAIAALG"
     assembly_gap    1439..1454
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1455..1999)
                     /locus_tag="PLV68_06445"
     CDS             complement(<1455..1999)
                     /locus_tag="PLV68_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HRE00920.1"
                     /translation="MPSANERALEKAKTLPADAIIFDLEDAVAPDAKVAARPNAVAAV
                     SSGDYGRRELTIRCNGLDTQWGADDVAAAAQSGASAVVIPKVSSLATLDEVSSRLSAA
                     GAPDSMTIWAMIETPTAIFDVRALAAHPRVSVLVMGTNDLARELRAGLVPGRHPLVPH
                     LAVALAAAREAGKVILDVVFN"
     gene            complement(2047..>2748)
                     /locus_tag="PLV68_06450"
     CDS             complement(2047..>2748)
                     /locus_tag="PLV68_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HRE00921.1"
                     /translation="MTPKVEGREDIHYVDRLLAQLEAKAGLTKPLLVHALLETARGVA
                     NVEAICGASPRMQGLSLGPADLAANRRMKTTRVGGGHPDYIVRVDPNPAEPDAPRATY
                     QQDLWHYTIARMVDACATHGILPFYGPFGDIKDTVACEDQFRNAYLLGCVGAWSLHPV
                     QIDIAKRVFSPRPSDVAQAQRVIDAMGDGTGAVMLDGKMEDDASVKQCKVVVQLAREL
                     AARDPHLAEAYGFAS"
     assembly_gap    2749..2886
                     /estimated_length=138
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2887..3100)
                     /locus_tag="PLV68_06455"
     CDS             complement(<2887..3100)
                     /locus_tag="PLV68_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HRE00922.1"
                     /translation="MRSARDFFKPLAVGAPQPLREIPFRPSRVIHFFPPQIDKVVAKL
                     PDVIPTVDIILGNLEDAIPMADKQAAR"
     gene            3211..>3449
                     /locus_tag="PLV68_06460"
     CDS             3211..>3449
                     /locus_tag="PLV68_06460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00923.1"
                     /translation="MTYLADRHITGFDAILFDAGGILMIPDPSVLMPLLVPYGASDDV
                     EAYVRAHYRGMAAKSAAGAGEKFWHEYNVEYCTAVG"
     assembly_gap    3450..3507
                     /estimated_length=58
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3508..3917
                     /locus_tag="PLV68_06465"
     CDS             <3508..3917
                     /locus_tag="PLV68_06465"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRE00924.1"
                     /translation="FLYRGAVEMLAALHRAGVPIGVVSNASGQIEEMLRRSGLCQVGD
                     GPLTPVRVIVDSHVVGVAKPDPRIFDHALGYFADIPLERIAYVGDSVTMDIGGAAAAG
                     LHPVLFDPYDDHPAATFARLRSLSDLIPAAHRA"
     gene            4086..>4312
                     /locus_tag="PLV68_06470"
     CDS             4086..>4312
                     /locus_tag="PLV68_06470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00925.1"
                     /translation="MNGPEGDAAASAGERTHPLLGMMVDAAQGRFPASDGGVTYLPPL
                     AGGREAVVAFSGHTVIASRLGPAELADLAPDG"
     assembly_gap    4313..4339
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4610..4798
                     /estimated_length=189
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4846..>5103)
                     /locus_tag="PLV68_06475"
     CDS             complement(4846..>5103)
                     /locus_tag="PLV68_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRE00926.1"
                     /translation="VVSKLMAKGEGQAEASMVKAYVCKAAEWVAREAMQIHGGYGYAE
                     EYAVSRLFVDARVLSIFEGADETLCLKVIARRLVDNANSRA"
     assembly_gap    5104..5114
                     /estimated_length=11
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5115..>5649)
                     /locus_tag="PLV68_06480"
     CDS             complement(<5115..>5649)
                     /locus_tag="PLV68_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HRE00927.1"
                     /translation="GWVLNGQKTWCTFAGMADVLMVLARSDADPKKGHRGLTIFLLEK
                     PRFTGHEFTFEQKRVVDGKEVVGKVTARAIDTIGYRGMHSYEVALEDWWVPAENLIGE
                     ADGLGKGFYLQMQGFENGRLQTAARAVGVMQASYEAALEYANNRAVFGNNIVEYQLTQ
                     AKLGRMAVLIQSGRQFAY"
BASE COUNT          761 a         1717 c         1776 g          881 t
ORIGIN      
        1 agaacgcgcc gagaccacgg acgtcaccga cggacgggtg cttgtcctgc atggctgaca
       61 acgccgggcc gatgatgtcg gtgccgatcc gacgggcgtg ctcgacgatg ccctcttcct
      121 tgaagatgtt gatgctcgcc accacgctgg cgcaggccaa cacgtgaccg ctgtaggtga
      181 gcccgccggg gaactggcga tccttgaacg tgtcggcgat ctcgtcgctg atcaccacgc
      241 caccgatcgg gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnngaac ccggccatca cctcgtcggc gatgtagacg
      361 atgccgaact cgtcgcagat ctcacgcacg ccctgcaggt agccgggcgg cggcaccagg
      421 atgccgttgg tgccaaccac ggtctcgagg atgatgcagg cgatcgtgtg gccgccttcg
      481 accatgatcg tggagcgcaa gtgggccaag gcgcgctcgg tctcctcctc tggcgtggac
      541 gcgtagaacg gactgcggta ggggtacggc ccccagatgt ggatcacgcc cgacacgccg
      601 gtctcgttcg gccaccgccg cggatcgccg gtgaccgcga tggcgccgcc ggtcgcaccg
      661 tggtagctgc ggtacgtgga gagcaccttc tggcgccctg tgtgcaggcg ggccaggcgg
      721 atggcgttgt cggtcgcgtc ggcgccaccg ttggtgaaga acaccttgtt caagccctcg
      781 ggagcgagct cggcgatgag ccgggccgcc tcgctgcgag cctcgacagc atgcccgggc
      841 gcgatcgtgc acagcttggc cgcctgctcc tggatcgccg ccaccagctt ggggtgctgg
      901 tggccgatgt tgacgttgac gagctggctg gagaagtcga ggtagcgcgt gccgtcctcg
      961 gtccagaacc agcttccctc gccgcccgtg accaccatcg ggttgagtgc gccctgcgac
     1021 gaccaactgt gaaagacgtg cttgcggtcg ttggcgtagg tggaggacgg tggagcgtcg
     1081 acggcggtca tgaggcaatc gtattcgaca cagctagtgg tgtacagaac ttgttctgtt
     1141 accgtacaaa gtgtcaatag gggtgcggga tgtgcggctg tggctcagcc gagcgcggcg
     1201 atcgcttccg cagtggccag ggtgcggcgg gcgttgtcga cgtggaggtt ctcgatcatc
     1261 cgattgtcga cggtgatcac gccgcgaccc tcgcgcaggc cctcttcgaa tgcgtcgatc
     1321 acgcggcggg cgtgttcgac ctcgtcctca ctcggtgccc acacctcgtt ggcgatgtcg
     1381 acctgaccgg gatggatcag cgtcttgccg tcgaagccca tctctacgcc ttgcacgcnn
     1441 nnnnnnnnnn nnnntcgttg aacacgacgt cgaggatgac cttgcctgcc tcgcgggcgg
     1501 cggccagcgc gaccgccaga tgcggcacca gcgggtgacg accgggcacc aacccggcgc
     1561 gcagctcgcg ggccaggtcg ttggtgccca tcaccagcac cgagacgcgc gggtgggccg
     1621 ccagggcgcg gacgtcgaag atcgcagtcg gcgtctcgat catcgcccag atcgtcatcg
     1681 agtccggcgc gccagcggcg ctcaggcgcg acgacacctc gtcgagcgtc gccagcgacg
     1741 acaccttcgg gatcaccaca gcactcgcgc ccgactgtgc cgcggcggca acgtcgtccg
     1801 cgccccactg ggtgtcgagg ccgttgcagc ggatggtcaa ctcgcgtcgg ccgtagtcgc
     1861 ccgacgacac cgcagccacc gcgttgggcc gagcagcgac cttcgcgtcg ggagccaccg
     1921 cgtcttcgag gtcgaagatg atcgcgtcgg cgggcagggt cttggccttc tccaacgcgc
     1981 gctcgttggc cgacggcatg tacagaaccg accggcgtgg ccgcaacgat gcgccctgcg
     2041 acgtgctcat gacgcgaacc cgtaggcctc ggccaggtgc gggtcgcggg cagcaagctc
     2101 gcgggccaac tgcacgacca ccttgcactg cttgacgctg gcgtcgtctt ccatcttgcc
     2161 gtcgagcatc accgcgcccg taccgtcacc catcgcgtcg atgacccgct gcgcctgggc
     2221 gacgtcgctc ggccgtgggc tgaagacgcg cttggcgatg tcgatctgca ccgggtgcaa
     2281 gctccacgca cccacgcagc cgagcaggta ggcgttgcgg aactggtcct cgcaggcgac
     2341 cgtgtccttg atgtcgccga agggtccgta gaacggcagg atgccgtgcg tcgcgcaggc
     2401 gtcgaccatg cgggcgatcg tgtagtgcca caggtcctgc tggtacgtgg cgcggggcgc
     2461 atcgggctcg gccgggttgg gatcgacgcg cacgatgtag tcggggtggc cgccgccgac
     2521 gcgggtggtc ttcatccgcc ggttggcggc caggtcggcg ggtcccaacg acaggccctg
     2581 catgcgcggc gacgcgccgc agatcgcctc gacgttggcg accccacggg ccgtctcgag
     2641 cagcgcgtgg accagcagcg gcttggtcag cccggccttg gcttcgagtt gggccagcag
     2701 gcggtcgacg tagtggatgt cctcccggcc ttcgaccttg ggggtcatnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnncgcg ggcggcctgc ttgtcggcca tcgggatcgc gtcctcgagg ttgccgagga
     2941 tgatgtcgac ggtggggatc acgtccggca gcttggcgac caccttgtcg atctgcggag
     3001 ggaagaagtg gatcacccgg ctgggacgga acgggatctc ccgtagcggc tggggagccc
     3061 cgaccgcgag cggcttgaag aagtcacgag cgctacgcac gtctcgacgt tactcagcag
     3121 tcggtggaac ggggaaacga gcagccgctg gtctgccgct gacgacgatg cagcgcggag
     3181 ccgtcgggcc gggtcggtac tctgcccggc gtgacgtacc ttgccgaccg gcacatcacc
     3241 ggcttcgatg cgatcctgtt cgatgcgggt ggaatcctga tgatccctga tccctccgtg
     3301 ctgatgccgc tgctggtccc gtacggggcg agcgatgacg tcgaggcgta cgtgcgagcg
     3361 cactatcgcg gcatggcggc gaagtccgcc gccggcgcgg gagagaagtt ctggcacgag
     3421 tacaacgtcg agtactgcac cgcggtcggn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnact tcctctatcg cggcgcggtc gagatgctcg
     3541 ccgcgttgca tcgtgctggc gtgccgatcg gtgtcgtgtc gaacgcgtcc gggcagatcg
     3601 aagagatgct gcgccgctcc ggactgtgtc aagtgggtga cggcccgctg acgccggtgc
     3661 gggtcatcgt cgacagccac gtcgtcggcg ttgccaagcc cgacccgcgc atcttcgacc
     3721 acgccctcgg atacttcgcc gacatcccgc tcgagcgcat cgcgtacgtc ggtgactcgg
     3781 tgacgatgga catcggcggt gccgcggctg ccggtctgca cccggtgctg ttcgacccct
     3841 acgacgacca ccccgcggca accttcgccc gcctccgctc gttgtccgac ctcatccccg
     3901 ctgcccaccg agcgtgaccc cgtagccggt gattccgcat agccggtgat tccgccgtcc
     3961 gcggagcgtg ggctgcccgg attccggtgc gtcggcactc cgcggcagcc ggattcaccg
     4021 gtggtcccgg ttcggcggtc cgtcatgggg aggggaccgg agcgaggagc cgccagactg
     4081 gcggggtgaa cggccctgaa ggcgatgctg cggcgagcgc gggggagcgg acgcacccgt
     4141 tgctcgggat gatggtcgac gccgcgcagg ggcggtttcc ggcctcggat ggcggggtga
     4201 cgtaccttcc gccgttggcc ggcgggcgcg aggcggtggt tgccttcagc gggcacacgg
     4261 tgatcgccag ccgcctcgga cctgctgaac tcgccgatct ggcgccggac ggnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnc tgttgcggtt ggcgccacgg cgaggtgggt gtactcgaca
     4381 ccaccttggt cgcccgcggc gccgacctcg attcgccaat cccgcgaacc gaccgttggg
     4441 acgaccatct gcgggtgcgg tacgcccgct cgctacgcga cgacgtcgag gtctacggag
     4501 acgagcgggg gttcttcacg cttgcacgcg gactggccgg tcggcgggag atgagccttg
     4561 cgatcgccga cgactccatc agccgtggcg tcggccgtca gttgttcgcn nnnnnnnnnn
     4621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncg
     4801 attcaccaca ccggtcgaac ccggctgccc cagcgcggcc cgccgtcagg cgcgggagtt
     4861 ggcgttgtcg accaggcggc gggcaatgac cttgaggcac agggtctcgt cggcaccttc
     4921 gaagatgctg agcacgcggg catcgacgaa caggcggctg accgcgtact cctcggcgta
     4981 gccatagccg ccatgaatct gcatcgcctc gcgggccacc cactcggcgg ccttgcacac
     5041 gtaggccttg accatgctcg cctcggcctg gccctcgccc ttggccatca gcttcgacac
     5101 gacnnnnnnn nnnnataggc gaactggcga cccgactgga tcagcacggc catacggccc
     5161 agcttggcct gggtcagttg gtactcgacg atgttgttgc cgaagacggc ccggttgttg
     5221 gcgtactcga gcgctgcttc gtacgacgcc tgcatcacgc cgacggcccg cgccgcggtc
     5281 tgcagccgcc cgttctcgaa tccttgcatt tgcaagtaga agcccttgcc gagcccgtcg
     5341 gcttcgccga tcaggttctc cgctggcacc caccagtcct ccaacgcgac ctcgtagctg
     5401 tgcatgccgc ggtagccgat ggtgtcgatg gcgcgtgcgg tgacctttcc gaccacctct
     5461 ttgccgtcga ccacgcgttt ctgctcgaag gtgaactcgt ggcccgtgaa cctcggtttt
     5521 tccaacagga agatggtgag gccgcggtgg cccttcttcg ggtccgcgtc cgaccgcgcg
     5581 agcaccatca acacatcggc catgccggcg aacgtgcacc aggtcttctg cccgttcagc
     5641 acccagccg
//
LOCUS       DAORNF010000126         5638 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_10615_length_5638_cov_1.756045, whole genome shotgun sequence.
ACCESSION   DAORNF010000126 DAORNF010000000
VERSION     DAORNF010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 5638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5638
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10615_length_5638_cov_1.756045"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>252)
                     /locus_tag="PLV68_06485"
     CDS             complement(<1..>252)
                     /locus_tag="PLV68_06485"
                     /inference="COORDINATES: protein motif:HMM:NF013253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HRE00928.1"
                     /translation="CSTHGGRQANGGLPALEALPGVVEAADGKPVIFDSGVRSGPDVL
                     TALAMGASAVGIGRPYAYALAAAGAAGVEHLVRCLMAELD"
     assembly_gap    253..334
                     /estimated_length=82
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<335..1080)
                     /locus_tag="PLV68_06490"
     CDS             complement(<335..1080)
                     /locus_tag="PLV68_06490"
                     /inference="COORDINATES: protein motif:HMM:NF013253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HRE00929.1"
                     /translation="MANFGNWQLSIYFNGLVGVRPEHPMTYADWERAAHEALSEQMWS
                     YVSGGAGDERTQRANVEAFGRWALWPRILAGAAHRDVTVDLFGRTRPTPLMFAPVGVI
                     GLCDADGHGDVAVARASAATGVPMIASTLMQDPLEDVAAAWGDTPGWFQLYPPKDREL
                     TASFVRRAEAAGYEAIVVTLDTLTLGWRPRDLAIASFPQLQGLCLANYFSDPVFRAQL
                     AKPPEEDVQAATGLWAMIFGNPGLDWDDLN"
     gene            complement(1252..>1463)
                     /locus_tag="PLV68_06495"
     CDS             complement(1252..>1463)
                     /locus_tag="PLV68_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00930.1"
                     /translation="AEIVGGPNRQQLGQLSGRRSFRLDGGSRSDGTPDRVLATWIVRA
                     RNLTGLEVTATHPRAGTVRANVTPQ"
     assembly_gap    1464..1586
                     /estimated_length=123
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1587..2940)
                     /locus_tag="PLV68_06500"
     CDS             complement(<1587..2940)
                     /locus_tag="PLV68_06500"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HRE00931.1"
                     /translation="MPSPLVFDRFIRYAELESTLAALAAEHPDLVTVEQYGTSFEGRA
                     LRLVTITDRSTGAPETKPAHWVDANIHAVEVTGGAAALHLIDWLVTAAERGDQTTLTA
                     LATRTFYVVPRVNPDGVEAALGDAPVFRRSSMRPWPYHQPHFWPGLDVRDIDGDGLVL
                     TMRIADPDGAWVEHPDDSRVMMPVPVDGIVADGTTRYRLLAEGIVHDFDGFTVPQPKS
                     PQSLDMNRNFPAGWGTGVRGSGDHPLSEPEIDALVRAIVARPNICGYNAFHTAGGVLL
                     RPSSTASDSSLPPVDVWTWKQLGERGTALTDYKVHSVFEDFTWDKSETMSGAADDWAY
                     EHLGLYGWTTEFWDAAYRATGTRSSTDVWYLGPTVDEELAIARWSDQHGTYFHPWRAF
                     DHPQLGPVEIGGCDWFHVVTNAPLPVLVDEIRPHAEFAVHQALAAPCLAIRTAHAVAL
                     G"
     gene            3003..3749
                     /gene="gpmA"
                     /locus_tag="PLV68_06505"
     CDS             3003..3749
                     /gene="gpmA"
                     /locus_tag="PLV68_06505"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HRE00932.1"
                     /translation="MTGTLVLLRHGQSEWNELNLFTGWHDVALTELGRQEAATAGRTM
                     AEAGLRFDVSHTSVLTRAVHTQHLALEAMDMMWLPVQRNWRLNERHYGALQGLDKKQT
                     TERHGAEQVKLWRRSYDVPPPPVGLDSPEHPRNDPRYRLLPRDVLPASECLADVVTRV
                     LPYWYDVVAPQLLAGLDVLITAHGNSLRALVKHLEGISDDDIAELNIPTGAPRQYRFD
                     AALGVTSADYLGDAAAVAAAAAAVANQAGT"
     gene            3831..5627
                     /locus_tag="PLV68_06510"
     CDS             3831..5627
                     /locus_tag="PLV68_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRE00933.1"
                     /translation="MSPHPFVRLWHHARNHRRRLVLAGVMSTINKACDVVPELLIGVA
                     IDVVVRGNTSFLSSLFGVSDRFDQLLLLAALNIGAWLLESLTDFLAHVLWRNLAQAVQ
                     HDLRLATYRHVQDLEVAWFEDNTSGGLLAVLNDDINQLERFLDHGPDDILQTFWNIVF
                     VGAVFLVASPKLWMLAFVPIPIIVLGSVRYQRRLEPLYDGVRERVADLSALLSGNLGG
                     IATIKAFTAEQREAERLDAVSQAYVDANGRAIRFSSAFIPLIRMVILAGFTCTLVIGG
                     RMVIDNDLEVGVYSVLVDMTQRLLWPLTRLGETLDLYQRSMASVRRVSDLLETTPKMV
                     NGSGTLTSPVRGEIDLRDIRFGYGDGGNAGDDVLRGISMHIPAGETHAVVGATGAGKS
                     TITKLLLRLHDPRAGVVSIDGSDVRDLSYVSLRSTIGYVSQDVFLFQGTVRDNLRFGR
                     PDATDAQVEAAAIAAEAHEFIVKLPDGYDTIVGERGQKLSGGQRQRLSIARAIVRDPA
                     ILVLDEATSAVDNETEAAIQRSLARVAQNRTAVVIAHRLSTVRDADKIWVLEGGQIIE
                     SGTHDELVATHGLYAALWRVQTGSAAREHIAD"
BASE COUNT          867 a         1900 c         1824 g          842 t
ORIGIN      
        1 gtcgagctca gccatcagac agcgcacgag gtgctcgacc ccggctgcac cggccgcggc
       61 gagcgcgtag gcgtacggac gaccgatacc gacggcactg gcacccatcg ccagcgcggt
      121 caacacgtcc ggaccgctgc gcacgcccga atcgaagatc accggcttgc cgtcggccgc
      181 ttcgaccaca ccgggcaacg cttccagcgc cgggagtccg ccgttggctt ggcgaccgcc
      241 gtgggtcgag cannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncagttc aggtcgtccc aatcgaggcc
      361 ggggttgccg aagatcatcg cccacagtcc ggtcgcggct tggacgtcct cctccggcgg
      421 cttggcaagt tgagcgcgga acaccgggtc gctgaagtag ttggccaggc acagcccttg
      481 cagttgggga aagctggcga tggcgaggtc gcgcggtcgc caccccaggg tcaaggtgtc
      541 gagggtgacc acgatcgcct cgtaaccggc ggcctcggca cggcggacga agctggccgt
      601 cagctcgcgg tccttcggtg ggtagagctg gaaccagccg ggcgtgtcgc cccacgccgc
      661 ggcgacgtcc tcaagcgggt cctgcatcaa cgtggacgcg atcatcggaa caccggtcgc
      721 ggccgaagct cgcgccacgg caacgtcgcc gtgaccgtcg gcgtcacaca gtccgatcac
      781 gccgaccggc gcgaacatca gcggcgtcgg ccgggtgcgg ccgaacagat cgaccgtgac
      841 gtcgcgatgc gcggcaccgg ccaggatgcg cggccacagc gcccaccggc cgaacgcctc
      901 gacgttggcg cgctgggtgc gttcgtcgcc cgcaccgccc gagacgtacg accacatctg
      961 ctcgctcaac gcctcgtggg cagcacgttc ccaatcggcg taggtcatcg gatgttcagg
     1021 gcggacgccc accaacccgt tgaagtagat cgacagttgc cagttcccga agttggccat
     1081 gccgcatcgt acgacgcccg ctccacccaa ccagcggtcg cgcgcgtccg ccgtcccatc
     1141 cccgtcaccg cgacaccgca acgccggtca atccggctgc cgcggagtac ggatttgccg
     1201 ggttccgggg agtccatgct ccgcggacgg ccgattcacc gggcaggtgg gctattgggg
     1261 cgtgacgttg gcgcggactg tgccagctct gggatgggtt gcggtcactt ccaggccggt
     1321 gaggttgcgg gcgcgcacga tccaggtggc gagcacgcgg tcgggcgtgc cgtcgctccg
     1381 gctgccgccg tcgaggcgga acgaacggcg tccggagagc tgaccgagct gttggcggtt
     1441 cgggccgccg acgatctcgg catnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnncgcc caacgcaacc gcgtgcgcgg tgcgaatcgc
     1621 caggcacggt gcagcgagcg cctgatggac ggcgaactcg gcgtggggac ggatctcgtc
     1681 caccagcacc ggcaagggcg cgttggtgac gacgtggaac cagtcgcagc cgccgatctc
     1741 gaccggcccg agctgggggt gatcgaaggc gcgccacgga tggaagtacg tgccgtgctg
     1801 gtcgctccag cgagcgatcg ccaactcctc gtcgaccgtc ggccccagat accagacgtc
     1861 ggtgctcgac cgcgtgccgg tcgcacggta ggcggcgtcc cagaactcgg tcgtccaccc
     1921 gtacaagccg aggtgttcgt aggcccagtc gtcggcggca ccactcatgg tctcgctctt
     1981 gtcccacgtg aagtcctcga acacggagtg gaccttgtag tcggtcaacg ccgtgccccg
     2041 ctcgcccaac tgcttccacg tccacacgtc gaccggcggc agtgacgaat cgctcgcagt
     2101 gctggacggg cgcagcagca ctccccctgc ggtgtggaac gcgttgtacc cgcagatgtt
     2161 gggccgggcg acgatcgccc gcaccaacgc gtcgatctcc ggttcgctca gcgggtggtc
     2221 gccgctgcca cgcaccccgg taccccaacc ggccgggaag ttgcggttca tgtccaacga
     2281 ctgcggcgac ttcggctgcg gcaccgtgaa cccgtcgaag tcgtgaacga tgccctcggc
     2341 caacaaccgg tagcgcgtcg ttccgtctgc aacgatcccg tcgaccggga cgggcatcat
     2401 cacccgcgag tcgtcggggt gttcgaccca cgcgccgtcg gggtcggcga tccgcatcgt
     2461 cagcacgagc ccgtcaccgt cgatgtcacg cacgtcgaga ccgggccaga agtgcggctg
     2521 gtgatacggc cacggccgca tgctgctgcg ccggaagacc ggtgcgtcac cgagcgcggc
     2581 ttcgacgcca tcggggttca cccgcggcac cacgtagaac gtgcgggtcg ccagcgcggt
     2641 caacgtcgtc tggtcgccgc gctcggcagc cgtgaccaac cagtcgatca ggtgcagtgc
     2701 tgccgccccg ccggtgacct cgaccgcgtg gatgttggca tcgacccaat gggccggctt
     2761 ggtctcgggc gctccggtgc tgcgatcggt gatcgtcacc aagcgcagcg cgcggccttc
     2821 gaacgaagtg ccgtactgct cgacggtgac gagatcgggg tgttccgcgg ccaaggcagc
     2881 aagcgtcgat tccagttcgg cgtagcgaat gaagcggtcg aacaccagcg gagacggcat
     2941 ggccgcggac attacagccg ggtcgctggg agcatcgacc ggtgcttgcc tacgatgacg
     3001 cgatgaccgg aaccctcgtg ttgttgcgcc atggccagag cgaatggaac gagctcaacc
     3061 tgttcaccgg ctggcacgac gtggccttga ccgagctcgg tcgccaagag gcggccacgg
     3121 cgggtcgcac catggccgag gccgggttgc gcttcgacgt ctcgcacacc tcggtgctga
     3181 cccgcgccgt gcacacccag cacctcgcct tggaagcgat ggacatgatg tggctgccgg
     3241 tgcagcgcaa ctggcgcctc aacgagcgtc actacggcgc gctgcaaggc ctggacaaga
     3301 agcagaccac cgagcgccac ggcgccgagc aggtcaagtt gtggcggcgc agctacgacg
     3361 tgcccccacc gccggtcggc ctcgacagcc ccgagcaccc gcgcaacgac ccccgctacc
     3421 ggctgttgcc gcgtgacgtc ctgcccgcgt ccgagtgcct ggccgacgtc gtcacccgcg
     3481 tgctgcccta ctggtacgac gtcgtcgcgc cgcagctgct ggccggtctc gacgtgttga
     3541 tcaccgccca cggcaactcg ctgcgcgcgc tggtcaagca cctcgagggc atctccgacg
     3601 acgacatcgc cgagctcaac atccccactg gcgccccgcg ccagtaccgc ttcgacgccg
     3661 cgctcggcgt caccagcgcc gactacctgg gcgacgcggc cgccgtcgcg gcagcagcgg
     3721 ccgctgttgc caaccaggcc ggcacctgac ccacacgtca cgcgcgcacc gccgcggcac
     3781 ggagaaatgg tgggcgccgg cgcggtgtgg tcggtacggt gcctggacgt atgagtccgc
     3841 atcccttcgt ccggctttgg caccacgctc gcaatcaccg ccggcggctg gtgctggccg
     3901 gggtgatgtc cacgatcaac aaggcctgcg acgtcgtccc cgagttgctg atcggcgtcg
     3961 cgatcgacgt cgtggtccgt ggcaacacgt cgttcctgtc gtcgctgttc ggcgtgtccg
     4021 accgcttcga ccaactgctg ctgctcgccg cgctcaacat cggggcgtgg ctgttggagt
     4081 cactgaccga cttcctggcc cacgtgctgt ggcgcaacct ggcccaagcg gtgcagcacg
     4141 atctgcgcct ggccacctat cgccacgtgc aagacctcga ggtcgcctgg ttcgaggaca
     4201 acaccagcgg cgggttgctg gcagtgctga acgacgacat caaccaactg gagcggtttc
     4261 tcgaccacgg tcccgacgac atcctgcaga cgttctggaa catcgtcttc gtcggcgcgg
     4321 tgttcctggt ggcctccccc aagctgtgga tgttggcgtt cgtgccgatc ccgatcatcg
     4381 tgctcgggtc ggtgcgctac cagcgccgcc tggaaccgct ctacgacggc gtgcgtgaac
     4441 gcgtcgccga cctgtcggcg ttgttatcgg gcaacctcgg cggcatcgcg acgatcaagg
     4501 ccttcaccgc cgaacagcgc gaggccgagc gactcgacgc ggtgtcgcag gcctacgtcg
     4561 acgccaacgg ccgggcgatc cggttctcgt cggcgttcat ccccttgatc cggatggtga
     4621 tcctggccgg gttcacctgc acgctcgtca tcggcggacg gatggtcatc gacaacgact
     4681 tggaagtcgg cgtgtactcg gtgctggtgg acatgaccca gcggctgctg tggccgctga
     4741 cccgcctggg cgagacgctc gacctgtacc aacgttcgat ggcgtcggtg cgccgggtca
     4801 gcgacctgct ggagaccacg cccaagatgg tcaacggcag cggcacgttg acatcgccgg
     4861 tgcgcggcga gatcgacctg cgcgacatcc gcttcggata cggcgacggt ggcaacgctg
     4921 gtgacgacgt gctgcgcggc atctcgatgc acatccccgc cggtgaaacc cacgcggtgg
     4981 tcggcgcgac cggtgcgggc aagtcgacca tcaccaagct gttgctgcgg ttgcacgatc
     5041 cgcgtgcggg cgtggtcagc atcgacggaa gcgacgtgcg cgacctgtcg tacgtgtcgc
     5101 tgcggagcac gatcggctac gtcagccaag acgtgttcct gttccaaggg accgtgcgcg
     5161 acaacctccg cttcgggcgt cccgacgcga ccgacgccca ggtcgaggcc gcggcgatcg
     5221 cggccgaggc ccacgaattc atcgtcaagc tgcccgacgg ctacgacacc atcgtcggcg
     5281 agcgcggcca gaagctgtcg ggcggtcaac gccagcggtt gtcgattgcc cgcgccatcg
     5341 tgcgcgaccc ggcgatcctg gtgctcgacg aagcgacctc cgcggtcgac aacgagaccg
     5401 aagcagcgat ccaacggtcg ctcgcccggg tcgcgcagaa ccgcaccgcc gtcgtgatcg
     5461 cccaccgcct gtcgaccgtg cgcgacgccg acaagatctg ggtgctcgaa ggcggtcaga
     5521 tcatcgagtc gggcactcac gacgagctcg tcgcgacgca cggcttgtac gccgcactgt
     5581 ggcgggtgca gaccggctcg gccgcccgcg aacacatcgc cgactgaccc gccgccga
//
LOCUS       DAORNF010000127         5602 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_10698_length_5602_cov_1.688120, whole genome shotgun sequence.
ACCESSION   DAORNF010000127 DAORNF010000000
VERSION     DAORNF010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 5602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5602
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10698_length_5602_cov_1.688120"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>245
                     /locus_tag="PLV68_06515"
     CDS             <1..>245
                     /locus_tag="PLV68_06515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00934.1"
                     /translation="TVTDSTEPNDAHDVAAPASPASGAVAAGATASEITGAPAGRDPY
                     VDFLRAFSLLVVVAWHWVFTIIIWRDDGPHATNPIGF"
     assembly_gap    246..370
                     /estimated_length=125
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <371..>591
                     /locus_tag="PLV68_06520"
     CDS             <371..>591
                     /locus_tag="PLV68_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00935.1"
                     /translation="KSLAIPALALIGVWLVGGTALGIVFDLEWIGRATLLVISPLWFL
                     AVYLVLILLLPVFLWLHRRFDVLVLVWLG"
     assembly_gap    592..753
                     /estimated_length=162
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <754..1203
                     /locus_tag="PLV68_06525"
     CDS             <754..1203
                     /locus_tag="PLV68_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00936.1"
                     /translation="GLAGLVGSGLYPGSMVGVPGERSNMAPPTLCIVALVLFQAGVAE
                     AIRPWMQARLPRPGWARLNTVINRFSMPLFLFHTTGMALSRAVTYAVAGGENEPQHPT
                     GWWWLSRPVSFVLPLLFTLPVILLFGKRWTKADVTTRRLPGKPAAAS"
     gene            complement(1210..2154)
                     /locus_tag="PLV68_06530"
     CDS             complement(1210..2154)
                     /locus_tag="PLV68_06530"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HRE00937.1"
                     /translation="MQVITDLSEQPWPGERSVVTIGAYDGVHLGHRAVIDEVRQLADE
                     TGSRSVVITFDRHPASVVRPESAPLLLTDLEQKLELLASTGVDAAVVIRFDQEQARET
                     ALGFVQRVLVRGLHTSIVVVGADFHFGRSREGNVDALREWGVADDFTVIGHELVPRAD
                     GVDEPISSTAIRRALAGGQVELAAGLLGRPYEVRGPVVRGDQRGRLLGFPTANVEVPN
                     IICLPADGVYAGFYIRPGGDRIPCAVNLGRRPTFYEHAEHSLLEAHLLDFDDDLYGQR
                     ARVQLAHFLRSERKFDGIDALIAQLKNDVDHARDVLGA"
     assembly_gap    2293..2361
                     /estimated_length=69
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2362..>2710)
                     /locus_tag="PLV68_06535"
     CDS             complement(<2362..>2710)
                     /locus_tag="PLV68_06535"
                     /inference="COORDINATES: protein motif:HMM:NF013660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00938.1"
                     /translation="RPLYDLARQGLEVERAARPVTISRFDLFPTGGPLVWRAEVECST
                     GTYIRSLADDLGRLLGGGAHLRNLRRTRVGRFTLADAQPIDEVVLAPVAAAVAHLDSA
                     SVDERGADPVAHGR"
     assembly_gap    2711..2720
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2721..3109)
                     /locus_tag="PLV68_06540"
     CDS             complement(<2721..3109)
                     /locus_tag="PLV68_06540"
                     /inference="COORDINATES: protein motif:HMM:NF013660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00939.1"
                     /translation="MAVIDKPAGITSHDVVNRLRRHLGERRIGHAGTLDPSATGVLVV
                     CVGNATRLMRFLSGADKAYTGEIVLGATTTTLDADGDVVARFDMGDVTIDAGRAAVAA
                     HLHQHIWPALADPAFPRPKIRRFALARA"
     gene            complement(3142..3609)
                     /gene="rbfA"
                     /locus_tag="PLV68_06545"
     CDS             complement(3142..3609)
                     /gene="rbfA"
                     /locus_tag="PLV68_06545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HRE00940.1"
                     /translation="MAKPNRRRSNSTSQHRYPREARLNQSLRQVIAEELVAIDDEALD
                     LVTITQIDVDSEMNRATVYFDSLRGEDGDGEILEALQRHRSRLQSAVGRQIRSKKTPI
                     LSFAPDDVIRSAERIDHILRTNPSPLVPDSDVPDSDVPDSIVDGEPADGERDA"
     gene            complement(3669..>5274)
                     /gene="infB"
                     /locus_tag="PLV68_06550"
     CDS             complement(3669..>5274)
                     /gene="infB"
                     /locus_tag="PLV68_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HRE00941.1"
                     /translation="LLVDPGQQEEMELQALFDDSDDEDETLQVPRAPVVTVMGHVDHG
                     KTTLLDKIRKANVVAGEAGGITQHIGAYKVTTDGGAEVTFIDTPGHAAFTKMRARGAQ
                     VTDIVVLMVAADDGVMPQTREAIDHARAADVPIVVALNKIDRDNADPQRVLAQLAEHE
                     LVPESWGGDTIVVEMSAVQELGIDDLLDQLAAVAELEELTANPTGRAKGIVLEAKLDT
                     GRGPVATILVDKGTLNVGDPIVAGAAWGRVRALINDKGEQIKQAGPSTPVQVLGLSAV
                     PNAGDEFRSANNEKTARIVGESRAQRAVLINQRGDARVQRGVKLEDIFSQIQAGEVAT
                     LNLVLKADVNGSLEAVTESLRRLERETVKLAFVSRGVGGISENDIMLAATTNATLIGF
                     NVRPDRKAREMAASEGVEIRTYEIIYKLLEDIERAMVGMLAPEFEEVVTGEAEVREVF
                     RVPRIGGIAGCYVRSGTITRGSKVRFLREGVIIWKGSISSLKRFKEDAREVREGFECG
                     IGLSDFQDLKPGDLIETYEDREIPRV"
     assembly_gap    5275..5318
                     /estimated_length=44
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5319..>5602)
                     /locus_tag="PLV68_06555"
     CDS             complement(<5319..>5602)
                     /locus_tag="PLV68_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HRE00942.1"
                     /translation="PGGTGPRPSGQRRAPRKGKSRRRRRDFNELQPQSMGNYTNSDAP
                     VPEGTIIVERGVSAQEFAPKLNRTAADVIRFLLNNGEMILGSHVPNDCLG"
BASE COUNT          782 a         1729 c         1791 g          890 t
ORIGIN      
        1 cacagtgacc gacagtaccg agcccaacga cgcccacgac gtcgcggctc ccgcttctcc
       61 ggccagcggt gccgtggcgg cgggcgcgac agccagcgag atcacgggtg ctccagccgg
      121 acgtgacccg tacgtcgact ttctgcgggc gttcagcctg ctcgtcgtcg tggcctggca
      181 ctgggtgttc accatcatca tctggcgcga cgacggcccg cacgcgacca acccgatcgg
      241 gttcannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn tcaagagcct ggcgatcccg gcgctggcgc tgatcggtgt gtggctggtc
      421 ggcggcaccg cgctcggcat cgtgttcgac ttggagtgga tcggccgggc caccctgttg
      481 gtgatcagcc cgctgtggtt cctggccgtc tacctcgtgt tgatcctgct gctcccggtc
      541 tttctctggc tgcaccgccg cttcgacgtg ctggtgctgg tgtggctggg cnnnnnnnnn
      601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnggtttgg cgggcctggt cggttccggg
      781 ctgtatccag gttcgatggt cggcgtacca ggcgaacgca gcaacatggc cccgccgacg
      841 ctgtgcatcg tggctctggt gctgttccag gccggagtcg cggaagcgat ccggccgtgg
      901 atgcaggcca ggctccctcg gccaggttgg gcacggctca acaccgtgat caaccggttc
      961 tcgatgccgc tgtttctgtt ccacaccacc ggcatggccc tcagccgggc ggtgacctat
     1021 gccgtcgcag gtggcgagaa cgagccgcag cacccgacgg gatggtggtg gctgtcgcgg
     1081 cccgtgtcgt tcgtgttgcc gttgctgttc acgctgccgg tcatcctgct gttcggcaag
     1141 cgctggacca aggccgacgt cacgaccagg cgactacccg gcaagcccgc cgcggcgagc
     1201 tgacgcgagt caggccccga gcacgtcgcg ggcgtggtcg acgtcgttct tcaactgggc
     1261 gatcaacgcg tcgatgccgt cgaacttgcg ctcgctgcgc aggaagtggg cgagctgcac
     1321 ccgggcccgc tggccgtaga ggtcgtcgtc gaagtcgagc aggtgcgctt cgagcagcga
     1381 gtgctcggcg tgttcgtaga acgtgggacg gcggccgagg ttgaccgcgc acgggatccg
     1441 gtcaccgccg ggcctgatgt agaacccggc gtacacaccg tcggcaggca ggcagatgat
     1501 gttgggcacc tcgacgttgg cggtgggaaa gcccaacagt cgaccgcgct ggtcgccgcg
     1561 caccaccggg ccacgcacct cgtacggacg tcccaggagt ccagcggcca gttcgacctg
     1621 tccaccggcc agcgcgcgcc ggatcgcggt ggagctgatc ggctcgtcga ccccgtcggc
     1681 gcgcggcacc agctcgtggc cgatcacggt gaagtcgtcc gcgacgcccc actcgcgcag
     1741 tgcatcgacg ttgccctcgc gcgaacgtcc gaagtggaag tcggcgccga ccaccacgat
     1801 gctggtgtgc aggccgcgga cgagcacgcg ctgcacgaat cccagcgcgg tctcgcgggc
     1861 ctgctcctgg tcgaagcgaa tcaccacagc ggcgtcgacg cctgtggacg cgagcaactc
     1921 cagcttctgc tcgaggtcgg tcaacagcaa cggagctgat tcgggccgca ccacgctggc
     1981 cgggtgccgg tcgaaggtga tgaccaccga gcgggaaccg gtctcgtcgg ccaactggcg
     2041 cacttcgtcg atcacggcgc ggtgaccgag atgcaccccg tcatacgctc cgatcgtgac
     2101 caccgagcgc tcgcccggcc acggttgctc ggagagatcg gtgatcacct gcacggcacc
     2161 gacgctacct gctcgatcgg acgcgtcacg tcctcgatcc tggttactca ccggttggcg
     2221 tggtcgccgg cggggctgcg cactggcccg cgagcaccac gctgggcttg gccaggccag
     2281 cccggaacgg ctnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nccgcccgtg tgcgacgggg tcggcaccgc gctcgtcgac
     2401 gctggcgctg tcgagatgcg ccaccgcggc tgcaaccgga gccaggacga cctcgtcgat
     2461 cggctgggca tcggccaacg tgaaacgccc gacccgggtg cggcgcaggt tgcgcagatg
     2521 cgctcccccg cccagcaagc gcccgagatc gtcggccagc gagcggatgt aggtaccggt
     2581 cgagcactcg acctcggccc gccacaccag cggcccgccg gtggggaaca gatcgaagcg
     2641 agagatggtc accgggcgcg ccgcgcgctc gacttcgaga ccctgccggg cgaggtcgta
     2701 gaggggacga nnnnnnnnnn gcccgggcga gggcgaagcg gcggattttc gggcggggga
     2761 acgccgggtc ggccagcgcc ggccaaatat gctgatggag gtgcgcggcg acggccgccc
     2821 ggcccgcgtc gatggtgacg tcacccatgt cgaaccgtgc cacgacgtcg ccgtcggcat
     2881 cgagggtggt ggtggtggca ccgaggacga tctcgccggt gtacgccttg tcggccccgg
     2941 acaagaaccg catcaggcgg gtcgcattgc cgacgcacac caccaacaca ccggtcgcgc
     3001 tgggatcgag ggtcccggcg tggccgatgc ggcgctcacc gagatggcgg cgcaagcggt
     3061 tgaccacgtc gtgactggtg atgccggccg gcttgtcgat caccgccacg ccgtgcacgg
     3121 taggcggctt gcgccgggcc atcaggcgtc gcgctcgccg tctgctggct cgccgtcaac
     3181 gatcgagtca ggcacgtccg agtccggcac gtccgagtca ggcaccagag ggctggggtt
     3241 ggtacgcagg atgtgatcga tccgctcggc cgagcggatc acgtcgtcgg gcgcgaacga
     3301 caggatcggc gtcttcttcg aacggatctg acgtccgacg gcggactgga ggcggctgcg
     3361 gtgacgctgc agtgcttcca ggatctcacc gtcgccatcc tcgccccgca acgagtcgaa
     3421 gtacacggtg gcgcggttca tctccgagtc gacgtcgatc tgggtgatcg tcaccaggtc
     3481 gagcgcttcg tcgtcgatgg ccaccagctc ctccgcgatc acctggcgca acgactggtt
     3541 gagccgggcc tcacgcggat aacggtgctg gctcgtgctg ttcgaacggc gacgattggg
     3601 cttggccatg gcgatcactc cctctcagac ggattggttg cagacgtgcc ggtgacggac
     3661 gtgtgagttc agacgcgcgg gatctcgcgg tcttcgtagg tctcgatcag gtcgccgggc
     3721 ttcaggtcct ggaagtcgct gaggccgata ccgcactcga agccctcgcg gacctcacgg
     3781 gcgtcttcct tgaagcgctt gagcgacgag atgctgccct tccagatgat gacgccctca
     3841 cgcaagaagc gcaccttgct gccacgcgtg atcgtgcccg aacgcacgta gcaaccggcg
     3901 atgccgccga tgcggggcac gcggaacact tcgcgcacct cggcttcgcc ggtgacgacc
     3961 tcttcgaatt cgggcgccaa catgccgacc atcgcccgct cgatgtcttc gagcagcttg
     4021 tagatgatct cgtaggtgcg gatctcgacg ccttcggagg cggccatctc gcgcgccttg
     4081 cggtcggggc ggacgttgaa gccgatcagg gtcgcgttgg tggtggcggc gagcatgatg
     4141 tcgttctcgc tgatgccacc cacgccgcgg ctgacgaagg ccagcttgac cgtctctcgt
     4201 tcgagacggc gcaggctttc ggtgaccgcc tccaacgaac cgttgacgtc ggccttcaac
     4261 accaggttga gggttgcgac ctcaccggcc tggatctggc tgaagatgtc ctcgagcttg
     4321 acaccgcgct ggacgcgggc gtcgccacgc tggttgatga gcacggcccg ctgagcacgc
     4381 gattcgccga cgatgcgggc agtcttctcg ttgttggccg agcggaactc gtcgcccgcg
     4441 ttgggcaccg ccgacagacc gagcacctgc acgggcgtgg agggaccggc ctgcttgatc
     4501 tgctcaccct tgtcgttgat gagtgcccgc acacgacccc acgccgcacc ggcaacgatg
     4561 gggtcgccga cgttgagcgt gcccttgtcg accaggatgg tcgcgacggg gccacgaccc
     4621 gtgtcgagct tggcttcgag cacgatgccc ttggcccgcc cggtggggtt ggccgtcagc
     4681 tcttcgagct cggccaccgc ggcgagttga tcgagcaggt cgtcgatgcc cagctcttgc
     4741 accgcggaca tctcgaccac gatcgtgtca ccaccccacg actcgggcac caactcgtgc
     4801 tcggccagtt gggccaggac gcgctgggga tcggcgttgt cgcggtcgat cttgttcagg
     4861 gcaaccacga tcggcacgtc ggccgcccgg gcgtggtcga tcgcttcccg ggtttgcggc
     4921 attacgccgt cgtcggcagc caccatcaac acgacgatgt cggtgacctg agcaccacgg
     4981 gcgcgcatct tggtgaacgc ggcgtgaccc ggggtgtcga tgaaggtgac ctcggccccg
     5041 ccatcggtgg tgaccttgta ggcaccgatg tgctgggtga tgccaccggc ctcgcccgcg
     5101 accacgttgg ccttgcggat cttgtcgagc agcgtggtct taccgtggtc gacgtggccc
     5161 atcacggtca cgacgggagc acgcggcact tggagggtct cgtcctcgtc gtcgctgtcg
     5221 tcgaacagcg cctgcagctc catctcctct tgctggccgg gatcgaccag caacnnnnnn
     5281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnac cgaggcaatc attagggaca
     5341 tgggacccaa ggatcatctc accgttgttg agcaggaagc ggatgacgtc tgcagcggtg
     5401 cggttgagct tcggagcgaa ctcctgggcc gacacaccac gttcgacgat gatcgtgccc
     5461 tcgggcaccg gtgcatcgct gttggtgtag ttgcccatcg actggggctg cagctcgttg
     5521 aagtcacgac ggcgacgacg gctcttgccc ttgcgtggtg cacggcgctg cccgctgggg
     5581 cgcgggcccg tgccgccggg ac
//
LOCUS       DAORNF010000128         5597 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_10714_length_5597_cov_1.259473, whole genome shotgun sequence.
ACCESSION   DAORNF010000128 DAORNF010000000
VERSION     DAORNF010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 5597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5597
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10714_length_5597_cov_1.259473"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="PLV68_06560"
     CDS             complement(<1..275)
                     /locus_tag="PLV68_06560"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006974 - cellular response to DNA damage
                     stimulus [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HRE00943.1"
                     /translation="MCGRFVNTQTAQDVADYFGASFEPPEAGGPDLPANFNVAPTQDV
                     LAVVADGSADTGDVRMSVRAFHWGFVPLWAKDVKIGSSMINARGETVA"
     gene            583..1065
                     /locus_tag="PLV68_06565"
     CDS             583..1065
                     /locus_tag="PLV68_06565"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRE00944.1"
                     /translation="MPANPTTTAVTTPAGATTTTVPGLVASAEKYTVKNNDTVYGIAS
                     KHNISPQALAQLNGWSDGINHAIHPGDQIAVPDGSSVSSSSTPSSSTAPSTTTTTPAP
                     TTTIAVGGTYVIKENDYLAKIAKETGTTIDGIIAVNGWTDGIKHLLIPGDTIKLPATS
                     "
     gene            complement(1097..>1441)
                     /locus_tag="PLV68_06570"
     CDS             complement(1097..>1441)
                     /locus_tag="PLV68_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00945.1"
                     /translation="DINLTTAVPVRIELPGYVAVPQGRFVLNIPSGAGLAANKTVLFK
                     GGVLARTIEIPGDIPGDADTKFTIGVEEVVVQLLLRIRTRTTVGSPEVISDALVQVNK
                     NGAFAVNSWSVQ"
     assembly_gap    1442..1482
                     /estimated_length=41
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1483..>2441)
                     /locus_tag="PLV68_06575"
     CDS             complement(<1483..>2441)
                     /locus_tag="PLV68_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00946.1"
                     /translation="PQRTVPTRTTCEKIADAFAEDEEKRPYGVVTTQATTTTPEWFSD
                     PARDEKIRFGVYPGGTAIQTDTALWYRDHGSLARLKGTIWTPNLPVHALSTRLPFADP
                     MPSGFGDCQVFFPGTYTQPVTISSAIPTYFTSGIYYFEQPVTITGDANVVVGGGSVEG
                     CTTDQEAAFYATNAPSTHNITGLGGTFVFGYQGRLIVDNSVPSTTGNGVSLVFNQRYV
                     HPDDVNSLPSASVNVMSVNGALAGSDLSNPLTDLLVAGVVEVPKSLAPTTPDESGTVA
                     FADATLNKYRPSVLIPPDLPPPPEPPEPEDPENPPTETTEPPV"
     assembly_gap    2442..2495
                     /estimated_length=54
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2711..2968)
                     /locus_tag="PLV68_06580"
     CDS             complement(2711..2968)
                     /locus_tag="PLV68_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00947.1"
                     /translation="MTVVTTTRNFPSGTFDVATETFVDLEIQPVNLSDLGAYNPGSWS
                     CMAGNQPHPFTLVDIPGSGWKGIKVRVSANEAVSCTQAVTQ"
     gene            3018..>3389
                     /locus_tag="PLV68_06585"
     CDS             3018..>3389
                     /locus_tag="PLV68_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00948.1"
                     /translation="MGGEAYSRGIDGHSWTTAPEWLGHWMGGDAADGVAIDEAFFDSG
                     FPTDPIVDAISQLRQERGTGYAIHLWSMGFGAGFAHHAETLRNDQIKVLIEDYTGDWE
                     THLSKWAHARAYGLADQSVFGI"
     assembly_gap    3390..3578
                     /estimated_length=189
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3579..>3839)
                     /locus_tag="PLV68_06590"
     CDS             complement(<3579..>3839)
                     /locus_tag="PLV68_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00949.1"
                     /translation="MATGRPHRNEGTGILFVPFIQIDATGGLADPGMDRGRRRDDLDP
                     AVGRQAREIVRLDEAAAPIEKLQGESDGEIVTEISYTELPVRG"
     assembly_gap    3840..3913
                     /estimated_length=74
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3962..>4666
                     /locus_tag="PLV68_06595"
     CDS             3962..>4666
                     /locus_tag="PLV68_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter-associated beta strand
                     repeat-containing protein"
                     /protein_id="HRE00950.1"
                     /translation="MAGPSAVQAKAGETSIERVFFADAGWSIDGDPVSQDFYTYSINS
                     EGAGTNTIHIGISARDVVPAIFRVGTGNTLVINGRVGAVRNSGGLRKFGLGELILNHT
                     NTFLGDVWIRGGTLTIGGSGSLGNGHYEAPVTIDRGCTMRYGSSSAQRLSAGIHGEGD
                     FVQAGPGSVTLEGESASTGTVSVRGGALVVNGPLAQAEVTVESAGVIGGVGPFGTTLD
                     VSGTLAPGAGGIGTLTA"
     assembly_gap    4667..4882
                     /estimated_length=216
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4883..5236
                     /locus_tag="PLV68_06600"
     CDS             <4883..5236
                     /locus_tag="PLV68_06600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00951.1"
                     /translation="NEIWLVRWSNYTAWSERHAAGGAMDLDHDHDGIANGVEYFMGAG
                     ANPAESPGFLEWPKSLFYEGIYGTDYWLESSPDFETWTKVPGNDPRLHDGGTLRYQVA
                     PNETRGFLRLHVAGP"
     gene            5367..>5597
                     /locus_tag="PLV68_06605"
     CDS             5367..>5597
                     /locus_tag="PLV68_06605"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HRE00952.1"
                     /translation="MKTETLCLHGGHSPDAVTHSRAVPVYRTSSFTFDSTEHAANLFA
                     LKELGNIYTRLMNPTTDVLERRVALLEGAPEMG"
BASE COUNT          890 a         1613 c         1631 g          889 t
ORIGIN      
        1 gccaccgtct caccgcgggc gttgatcatc gacgagccga tcttgacgtc cttggcccac
       61 aacggcacga agccccagtg gaaggcgcgc acgctcatcc gtacgtcacc cgtgtcggcg
      121 gatccgtcgg cgaccacggc gagcacgtcc tgggtaggcg cgacattgaa gttggcgggc
      181 agatcggggc caccggcctc gggcggctcg aacgacgcgc cgaagtaatc ggctacgtcc
      241 tgggccgtct gggtgttgac gaaccggcca cacatggcct gcacggtagc gaagtcggat
      301 cggtggcgag tgcggggtga ctggagtgac gggtcgcgcc gccctagcgt cgtggcgtgc
      361 atccccgacg agatcgaacc tgccgtctgc tcgcgcgcgc aggcgcgctc ggcgccgtcg
      421 tggtcgtcag caccggctgc atccagtcgg cgttgcgcga gtcgaccgag accacccttg
      481 ctcccctgac gtccgagttc gtcacgctca cgatcgcgcc cacgtcgacc acgactccag
      541 acgctgcgac cgccccgcca gccgaccgca gccgaacagg ccgtgccggc caaccccacc
      601 acgaccgcgg tgacgacgcc ggctggggcg acgacgacga ccgtgcccgg tctggttgcc
      661 agcgccgaga agtacacggt caagaacaac gacaccgtct acggcatcgc cagcaagcac
      721 aacatctcgc cccaggcgct ggcgcagttg aacgggtgga gcgacggcat caaccatgcg
      781 atccaccccg gcgaccagat cgccgtgccg gacggttcgt cggtgtcatc gtcctcgacg
      841 ccctcttcgt cgacggcgcc ctcgaccacc acgacgacac ccgcgccgac caccacgatc
      901 gcagtcggcg gtacctacgt gatcaaggag aacgactacc tcgccaagat cgcgaaggag
      961 acgggcacca cgatcgacgg catcatcgcc gtcaatgggt ggaccgacgg catcaagcac
     1021 ctgctgatcc ccggcgacac gatcaagctg cccgccacct cgtagtggca caccgggcga
     1081 ctgggacgcg gcgcgcctat tgcacactcc agctgttgac ggcgaacgca ccgttcttgt
     1141 tgacctggac gagcgcgtcg gagatcactt ccggcgagcc gacggtcgtg cgggtacgaa
     1201 tacgcaacag caactgcacg accacctctt cgacgccgat cgtgaacttg gtgtcggcat
     1261 cgccggggat gtcaccgggg atctcgatcg tgcgggccag cacgccaccc ttgaacagca
     1321 ccgtcttgtt ggcagccagg ccggcacccg aggggatgtt cagcacgaac cggccctgcg
     1381 gcaccgccac gtagccggga agctcgatgc gcaccggcac cgcggtggtc aggttgatgt
     1441 cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnccaccgga ggctcggtcg
     1501 tctcggtcgg cgggttctcc gggtcttccg gctcgggtgg ttccggcggc ggaggaaggt
     1561 ctggcgggat cagcaccgag gggcggtact tgttcagcgt cgcgtcggcg aacgctacgg
     1621 tgccggactc gtccggcgtg gtcggcgcga gcgacttcgg cacctcgacc accccggcca
     1681 cgagcaggtc ggtcaacggg ttggacaggt cggagccggc caacgcgccg ttgaccgaca
     1741 tgacgttgac gctggccgac ggcagcgagt tgacgtcgtc ggggtgcacg taacgctggt
     1801 tgaacaccag gctgacgccg ttgcccgtgg tcgagggcac cgagttgtcg acgatcaggc
     1861 gaccctggta gccgaacacg aacgtaccgc cgaggccggt gatgttgtgc gtcgagggtg
     1921 cgttggtggc gtagaaggcc gcttcctggt cggtggtgca cccttcgacc gagcccccgc
     1981 cgaccaccac gttggcgtca ccggtgatcg tcacgggctg ctcgaagtag tagatacccg
     2041 aggtgaagta cgtcggtatg gcgctgctga tcgtgaccgg ctgggtgtag gtgccgggga
     2101 agaacacttg gcagtcaccg aagccggacg gcatcgggtc ggcgaacggc aaccgcgtgc
     2161 tcagcgcgtg caccggcaag ttcggtgtcc agatcgttcc cttcagccgt gccaggctgc
     2221 cgtggtcgcg gtaccacagc gcggtgtcgg tctggatggc cgtcccgccc gggtagacgc
     2281 cgaagcgaat cttctcgtcg cgagccgggt ccgagaacca ctccggagtc gtggtggtgg
     2341 cctgggtcgt caccacaccg tacggccgct tctcttcgtc ctccgcgaac gcgtcggcga
     2401 tcttctcgca ggtggtgcgc gtcggcaccg tgcgctgggg cnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncaggc cgagcttgac ggcctcttcc
     2521 cgctgggctc ggtcgaccgt caccgggctg gcccggttga ccgccatcga gaagtccatc
     2581 gtcgggatca cgatcagcga ggtgatcatg atcagcacca acacgagaat cagtgccgag
     2641 ccccggtcct gctggtccgg atcacgcggc ggcaaccaag cccgccgcgg ccgacgtacg
     2701 cgtcctcgcg tcattgcgtc acggcctgcg tacacgacac ggcttcgttg gcgctcacgc
     2761 gcaccttgat gcccttccac cctgagccgg gaatgtcgac caacgtgaac gggtggggtt
     2821 ggttacccgc catgcacgac catgaaccag ggttgtaggc accgaggtcc gacaggttga
     2881 ccggctggat ctccaagtcc acgaacgtct cggtggcgac gtcgaaggtg ccactgggga
     2941 agttacgggt ggtcgtcacg acggtcatgt ccgatgaccc tgcgtggaga catcgcggtg
     3001 tttccaccgt caagtgggtg ggtggcgagg cttactcgcg ggggatcgat ggtcactcgt
     3061 ggaccaccgc cccggaatgg ctcgggcatt ggatgggcgg cgacgcggcg gatggggtcg
     3121 ccatcgatga agcgtttttt gattccggct tcccgacaga tcccatcgtg gatgccattt
     3181 cccaattacg tcaggagcgt ggcaccggct acgccatcca tctttggtcg atgggattcg
     3241 gggccgggtt cgcccaccac gccgaaaccc tgcgaaatga tcagatcaag gtcttgattg
     3301 aagactacac gggcgattgg gagacgcatc tttccaagtg ggctcatgca cgcgcctacg
     3361 gacttgccga tcaatcggtc tttgggatcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncc cgcgaacggg aagctccgtg
     3601 tatgaaatct cggtaacgat ctcgccatcg gattcgccct ggagtttctc gatgggagcg
     3661 gcggcctcat ccaagcgcac gatctctcta gcctgccgcc cgacggccgg atcgagatca
     3721 tcccgcctcc ttccgcggtc catgccagga tcagccagcc ctccggtagc atcgatctgt
     3781 atgaagggaa cgaagagtat cccggtgccc tcgttccggt gcggccggcc ggtcgccatn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnnnnnn nnnccacccg gaaatctgga tagcggccgg ttcgcttggt tcgatggcag
     3961 tgtggcgggc ccttccgccg ttcaggcaaa ggccggcgag acatcgatcg agcgcgtctt
     4021 tttcgccgat gccggttgga gcatcgatgg agacccggtg agccaggatt tttacactta
     4081 cagcatcaac tccgaggggg ccggcaccaa taccattcac atcggcatct cggcacgcga
     4141 cgtggttccg gcgattttcc gggtgggcac ggggaacacg ctcgtaatca acggacgcgt
     4201 cggcgcggtg cgcaacagcg ggggattgag gaagttcggg cttggagaat tgatcctgaa
     4261 tcacaccaac accttcctgg gggatgtgtg gatccgtgga ggtaccctca cgatcggtgg
     4321 atcaggaagt ctcgggaatg gtcactatga agctcctgtc acgatcgacc gcggttgcac
     4381 gatgcgatat ggcagttcct cggcgcaacg cctttcagcg ggaattcatg gagaagggga
     4441 tttcgttcag gccggtcccg gctcggttac cttggaagga gaaagtgctt cgaccggcac
     4501 ggtatcggtc cgaggaggcg ccttggtcgt gaacggcccg ctcgcacagg ccgaagtcac
     4561 cgtagaatcc gctggagtga tcggaggggt cggacccttt ggtacgaccc tggacgtctc
     4621 cggtacgctc gcgcccggag ccggggggat cggaacgctc accgcgnnnn nnnnnnnnnn
     4681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4861 nnnnnnnnnn nnnnnnnnnn nnaacgagat ctggctcgta cgatggtcga attacaccgc
     4921 gtggtcggag cgacatgccg ccggaggggc gatggatctc gatcacgatc acgacgggat
     4981 tgccaacgga gtcgagtatt ttatgggcgc cggtgcgaac ccggccgagt cgcctggctt
     5041 cttggaatgg ccaaaaagcc tcttttacga aggaatctat ggcaccgact actggctgga
     5101 gtcctccccg gatttcgaga cctggaccaa ggtgcctgga aacgatcccc ggctccacga
     5161 tggtgggacg ctgcgttacc aggtcgcacc gaacgagacg cggggatttt tacgtcttca
     5221 cgtggccggg ccgtgagcgg catctccata gaccacggag atgaaggatt ttgcggggat
     5281 cctacctctc gcggggcccc cgaaaacgcc acggctttct cttgccagca ccggtcctct
     5341 ttgccaacct gttccacaca atccccatga aaaccgagac tctttgcctt cacggcggcc
     5401 acagccccga tgccgtcacc cattcccgcg ccgtgcccgt ctaccggacc agctccttca
     5461 cctttgattc gaccgagcat gccgcgaatc tctttgccct gaaggaactc ggaaacatct
     5521 acacccgtct catgaatccg acgaccgacg tcctcgagcg tcgcgtcgcg cttctggaag
     5581 gggcccctga aatgggc
//
LOCUS       DAORNF010000129         5510 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_10953_length_5510_cov_1.694959, whole genome shotgun sequence.
ACCESSION   DAORNF010000129 DAORNF010000000
VERSION     DAORNF010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 5510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5510
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10953_length_5510_cov_1.694959"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="PLV68_06610"
     CDS             <1..410
                     /locus_tag="PLV68_06610"
                     /inference="COORDINATES: protein motif:HMM:NF024792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HRE00953.1"
                     /translation="TKRCCDDRGSATIWMIGVTVAAFLMIGLVLDGGVMLRARSDAFA
                     VAGAAARVGAQQLDSDAAVEGEAVLDPVAAEQAALAHLAANGVTGSVVVTGDTVRVTV
                     TTTADLQLLSLVGGGSATFEATAEAQAVKVVPS"
     assembly_gap    417..426
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <427..>3040
                     /locus_tag="PLV68_06615"
     CDS             <427..>3040
                     /locus_tag="PLV68_06615"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRE00954.1"
                     /translation="VALVVSLAFVVGVPVLLVRYGEWPITSTPTIDQARDLVDTVVSD
                     TMVFGVLTVAAWIVWAGFTASLLVESVAAIRGGQAPHLSFAGPLQRSARVLVAAIVLA
                     VSLSHNHPAALAAGRASSPAPLPQRAVVAAQVTEPTHVPRGFHTPENAGAADAVPNAS
                     TPAGAVTVHRGDSAWGLAEQHLGDGMRWRELWELNRDRPQPDGRTWSNPQLIQAGWQI
                     LLPTSESPAAEPPVEPAMAEVHTVIDGETLSGIAEARLGDADRYGEIFELNRDVEQPD
                     GRRLTDPNLIVVGWELRIPTPEPASDAAEPTPAASGDATTGDVEADPPIQAPPTTTAP
                     VATTTSNVGSTAATTVPSVPADEQATPPPHPETRTASTEATEPNAISIDVAAADDPDE
                     SWLRSTAPVLAGISGATVLATGLLMRLRRSRRRDQLRGAHAVLVHSAAEQAVVAAADV
                     PLVRWAGQALAQMTSRIDRRNVGTRAPLAVELSDEGLEVLWDSPQPDAPAPWLAADQG
                     LAWRLSYDPDADIPADELPAAIPALVTVGDRDGRQLMIDLEAFGTIAVSGPPQRVDDF
                     LRAIALELSTDEDLADTYAVAIGLDGVPTTDRLMLADVETAGTRLTATCESVSKSLAS
                     DGAPTTFTYRCGNNAVHLEATVVIVIPSDGDATSLPTVGPRLGAALVIAADVPDAGAF
                     ITITDDGAAQLDPLGISFMAAAVPMETASVVERLSTETTVEQDGEPRTNGYHAQPIRV
                     EDIAAPATNGDAPALFELHDDEESDDDTDQPPRSPLDASLIVKVLGTPHVPDRPDLKR
                     RELILTVYLACRGRQVTASSVQDALWNGRAVQGKTVWNLVGRTRTALGTLPDGTWVLT
                     PSDRTRRMKGLSPQV"
     assembly_gap    3041..3248
                     /estimated_length=208
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3249..3483
                     /locus_tag="PLV68_06620"
     CDS             <3249..3483
                     /locus_tag="PLV68_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00955.1"
                     /translation="DDIDLAREAVAQGLRGLPGDEVLYRLRMKIEHHAGNLTGVSSAY
                     DELLSHLADFEADPSPSTVELRRELLGTTSPPS"
     gene            complement(3637..4086)
                     /locus_tag="PLV68_06625"
     CDS             complement(3637..4086)
                     /locus_tag="PLV68_06625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00956.1"
                     /translation="MSVTDFALVESARTITGDRQHERRFLDFVFDSVPMYAAVRANGV
                     DNITPIWLGDEWCRTAIHRLLGEAEPDAPDGRVSVYVCAECADLGCGAITVVVERDVD
                     TVSWRDWGYQNNYEDGFDAIDDLPDVTFDAAQYDSTLRGALDRLARR"
     gene            complement(4165..>4580)
                     /locus_tag="PLV68_06630"
     CDS             complement(4165..>4580)
                     /locus_tag="PLV68_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00957.1"
                     /translation="SHRPSGGEDVLAQYLPDGPPPTDPDQAQSAITAALAAFGQRSGD
                     DLPNVEQGAGLATMLDEALTQSPYQQATLAVTAVRFLNDHHAVTTCDVLVNGNRMLPN
                     WTARVVREQGRWKLARSAFAELLALAGVSVPPPHE"
     assembly_gap    4581..4679
                     /estimated_length=99
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4680..4886)
                     /locus_tag="PLV68_06635"
     CDS             complement(<4680..4886)
                     /locus_tag="PLV68_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00958.1"
                     /translation="MWVVGDTVNLVVDQETGVVLRYEAVAAGLPPLVVASWEHLEFDP
                     SIDDTVFDQAIPSAVNIRSTSEMLL"
     assembly_gap    5077..5172
                     /estimated_length=96
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          885 a         1835 c         1617 g          760 t
ORIGIN      
        1 ggacgaagcg ctgctgcgac gatcgtggct cagcgacgat ctggatgatc ggcgtcaccg
       61 tcgccgcgtt cttgatgatc ggcctggtgc tcgacggcgg cgtgatgctc cgggcccgca
      121 gcgacgcctt cgcggtcgcc ggagcagcag ctcgcgtcgg cgcacagcaa ctggactccg
      181 acgcggccgt cgaaggtgaa gcagtcctcg acccggttgc tgcggaacag gccgcactgg
      241 cccacctcgc ggccaacggc gtgaccggtt cggtcgtcgt caccggggac accgtgcgag
      301 tgacagtcac gacgaccgcc gacctccagc tcctcagcct cgtcggcggc ggcagcgcca
      361 cgttcgaggc caccgccgaa gcacaagccg tcaaggtggt gccatcgtga ctcgccnnnn
      421 nnnnnngtcg cgctcgtcgt ctcgctcgcg ttcgtcgtcg gtgtgcccgt gctgctcgtg
      481 cgctacggcg agtggcccat caccagcacg ccgaccatcg accaggcccg cgacctcgtc
      541 gacacagtcg tgtccgacac catggtgttc ggcgtgctca cggtcgccgc atggatcgtg
      601 tgggccggct tcaccgcatc cctgctcgtg gagagcgtcg ccgccatccg cggcggacaa
      661 gcgccacacc tgtcgttcgc cgggccgttg caacgatcag ctcgagtgct cgtcgctgcg
      721 attgtgctgg cggtcagctt gagccacaac cacccggccg cgctcgccgc cggtcgcgca
      781 tccagccccg caccactgcc acaacgggct gtcgtcgcgg cccaagtcac agaacccacc
      841 cacgtccctc gggggttcca tacacctgag aacgccggag ccgctgacgc cgtacccaac
      901 gccagcacgc cggcgggtgc cgtcacggtg caccgtggtg acagcgcctg gggcctggcc
      961 gagcagcatc tcggcgacgg catgcgctgg cgtgagctgt gggagctgaa ccgtgaccgc
     1021 ccgcaaccag atggacggac ctggagcaat ccccaactca tccaggccgg ctggcagatc
     1081 ctcctaccaa catccgagag ccccgctgcg gaaccccccg tcgagcccgc catggccgaa
     1141 gtgcacaccg tcatcgacgg cgagaccctc agcggcatcg ccgaagcgcg cctcggcgat
     1201 gccgaccgct acggggagat cttcgagctg aatcgcgatg tcgagcagcc ggacgggcga
     1261 cgcctcaccg acccgaacct gatcgtcgtc ggatgggaac tgcgcatacc cacgcccgaa
     1321 cctgccagtg acgccgccga gcccaccccg gcagcatcag gcgacgccac gactggagac
     1381 gtcgaggcag acccgcccat acaggcgcca ccgaccacga ccgccccggt agccaccacg
     1441 acgtccaacg tcgggagcac ggctgccacc accgtcccgt ctgtaccggc ggacgagcag
     1501 gccacaccgc cgccgcaccc ggaaacccga accgcctcga ccgaagcaac cgaacccaac
     1561 gcgatctcga tcgatgtggc cgctgccgac gatccggacg agtcatggct gcgatcgaca
     1621 gcacccgtac ttgcgggtat cagcggtgcg accgtgctgg caaccggact gctgatgcga
     1681 ctccgtcgat cccgacgacg ggaccagctc cgcggcgcac acgctgtcct ggtccactcc
     1741 gcggccgaac aggcagtggt agcagctgcc gatgtgccac tcgtgcgatg ggcaggccag
     1801 gcactcgcgc agatgacatc gcgaatcgac aggcgcaacg tggggacgcg cgctccactg
     1861 gcggtcgagc tatccgacga gggcctcgaa gtgctgtggg actcgccgca acccgacgcg
     1921 ccggcgccgt ggctcgctgc cgaccaaggc ttggcgtggc gactcagcta tgaccccgac
     1981 gctgacatcc cagcggatga gctccctgcc gctatcccgg cgctggtgac cgttggagat
     2041 cgcgacggac gacaactgat gatcgacctc gaagccttcg gcaccatcgc tgtctcaggc
     2101 ccacctcagc gagttgacga cttcctgcgg gcgatagcgc tcgagctgag caccgacgaa
     2161 gacctggccg acacgtacgc ggtcgccatc gggctggacg gcgtaccaac caccgatcgg
     2221 ctgatgctgg ccgacgtcga gacagctggc acccggctga ccgcgacctg cgagtcggtg
     2281 agcaagtccc tcgccagcga cggcgcgccg acaacgttca cctaccgctg cggcaacaac
     2341 gccgtacacc tcgaagcaac cgtcgtcatc gtgatcccca gtgacggcga cgcaacatcg
     2401 ttgccgacgg tcggccctcg cctgggtgcc gcgctcgtga tagccgctga tgtcccggac
     2461 gccggcgcgt tcatcaccat cacggacgac ggcgcggcgc aactcgatcc tctcggcatc
     2521 tccttcatgg cggccgctgt tccgatggag accgcctccg ttgttgaacg tctctcgacc
     2581 gaaacgaccg tcgagcagga cggagaaccg cgcacgaacg ggtaccacgc acaaccgatt
     2641 cgtgtcgaag atattgcagc gcccgctacc aacggcgacg ccccggcact gttcgagctg
     2701 cacgacgacg aagaatccga cgacgacacc gaccagccgc cacgctcgcc cctcgacgct
     2761 tcgctgatcg tcaaggtgct cgggacacct cacgtccctg accgccccga cctgaaacgg
     2821 cgagagttga tcctgaccgt gtacctcgcc tgccgaggcc gccaggtcac cgcttcctcg
     2881 gtccaagatg ccctgtggaa cggtcgcgcc gtgcagggca agaccgtgtg gaacctcgtc
     2941 ggccgcactc gcaccgctct cggaacgctg cctgacggca cctgggttct cacgccctcc
     3001 gaccgcacgc gtcgcatgaa aggcctctct ccgcaggtca nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnncg acgacatcga cctcgcccgc gaagccgtcg cccagggcct acgcggactc
     3301 cccggcgacg aagtcctgta ccggctccgc atgaagatcg aacaccatgc cggcaacctg
     3361 accggagttt cgagcgcgta cgacgaactg ctcagccacc tcgccgactt cgaagccgac
     3421 ccgtcgccat cgaccgtcga actgcgccgg gaacttctcg gcactacgag tccgcccagt
     3481 tgagccgccc ctacgtcggg gagggtcggc gaactacggg gtgctgccga cagctgagtg
     3541 gagtcctaca gggcgacttc ttcccgccag cggatatcgc tcgacctgcc cttcaacggc
     3601 acttcggtga cacccgcaca gatcggcaga tcggtgctac ctgcgggcga gacgatcgag
     3661 cgcaccccgg agcgttgagt cgtactgggc ggcgtcgaac gtcacgtccg gaaggtcgtc
     3721 gatggcgtcg aagccgtcct catagttgtt ctggtatccc cagtcgcgcc acgagaccgt
     3781 gtcgacgtcg cgttccacta cgacggtgat cgcaccacag ccgaggtctg cgcattcagc
     3841 gcacacgtac acggacacac gtccatctgg cgcgtctggt tccgcctcgc cgagcagtcg
     3901 gtggattgcc gtccggcacc attcgtcacc gagccagatc ggtgtgatgt tgtcgacgcc
     3961 gttggcgcgt accgcggcgt acatcggtac cgagtcgaac acgaagtcaa ggaatcgacg
     4021 ttcgtgttgg cgatcgcctg tgatggtgcg ggccgactcg acgagcgcga agtcggtcac
     4081 agacacccga gcacaatgcc tgctctggcg ctcccgcgca acgacgcgtc cctgaactgc
     4141 cggctctgtg cgggtgagga cgagctactc gtggggcggg gggacgctga cgccggcaag
     4201 agcgagtagc tcggcgaagg cgctgcgggc gagcttccag cggccctgct cgcgtacgac
     4261 gcgggcggtc cagttgggca gcatccggtt gccgttgacg aggacgtcgc aggtcgtcac
     4321 agcgtgatgg tcgttgagga acctgactgc ggtgacggcg agcgttgctt gctggtacgg
     4381 cgattgtgtc agcgcttcat ccagcatggt cgccagtcct gcaccctgct ccacgttggg
     4441 caggtcatcg ccggagcgtt gcccaaacgc ggcaagtgct gcggtgatgg cggactgtgc
     4501 ctggtcaggg tcggtcggcg gtggcccgtc gggcaggtac tgcgccaaca cgtcttcgcc
     4561 gccggacggg cggtgagacc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng
     4681 aggagcatct cgctggtgct gcggatgttc acggctgacg ggattgcctg gtcgaacacg
     4741 gtgtcgtcga tcgacggatc gaactcgagg tgctcccacg acgcgacgac gagtggcggc
     4801 aacccggcgg cgaccgcctc gtagcgcaac accacgccgg tctcctggtc gaccacgagg
     4861 ttgaccgtgt cgccgaccac ccacatcggg ctcggggagc gaacgccgat cggttcgcgg
     4921 tccaggcgta ccagccacgc tgcgcgcccg gaaaccacac gctcggctac gtctccgagc
     4981 tcgccggctc gcaggagggc acgcccgctc cacagcagtg ccggccactc cgcaagccgc
     5041 tcggcgggcc gccgcagagc accaggtccc ggatggnnnn nnnnnnnnnn nnnnnnnnnn
     5101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5161 nnnnnnnnnn nnatcgaaga ccaggtcgga gcgctcggca ggctcgccga accacatcgt
     5221 caccgagccg gactgattgc gccactcgct caccgctgcg tgatcggccc ggcactgcaa
     5281 cgatccccgc aggcccgcgc aacggggcgc cgcatgtaac gcctcaaacg cgtcgcccaa
     5341 caaccccacc ccacaattat cccacgacac tcaccagccc acgagacgca ccgatccagt
     5401 tacgcgccct caagcgcccc caaacgccgg ctgagcgacc cgtcgcggtg cgaggcggtc
     5461 ggcagacggt cgtgcgggtc atctgatggt cggtgacggg tcgtgctgtg
//
LOCUS       DAORNF010000130         5503 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_10968_length_5503_cov_1.772026, whole genome shotgun sequence.
ACCESSION   DAORNF010000130 DAORNF010000000
VERSION     DAORNF010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 5503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5503
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10968_length_5503_cov_1.772026"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2677
                     /locus_tag="PLV68_06640"
     CDS             <1..>2677
                     /locus_tag="PLV68_06640"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRE00959.1"
                     /translation="VDELDRAAFGILLPEIRDDFGIDLKTALGLVALASVAALALQVP
                     IAQIADRSKRVRMAIIGALVWAMFSGMTGLATGVVMLTIARSGSAVGKAVIDPTHNSL
                     LADYYPIHVRSKVFSTHRAANAVGAFVGPLSAGLLAYAFGWRTPFFVFIVPTVLFAVL
                     ALRMREPVRGRWERAAAGASDDVVNTEEIPASFAESWRTVQKVPSLRRMWWSLPFLAT
                     SLVGFVTLASVMYEREFDLDERARGIAAAIAEPFALVGIVVGTRIIPRRFLADMQGLI
                     RFLAGSAIVSSLAFAAFALSPNVVVAVAMNCVISAMLATIGPGILVAFSLAIPARARA
                     TGFSIGSLWVIPGLLVLPLVGWAADRWNVRVGMLVMLPLFVIGAFILRSMSTTINADI
                     AQVWKATAARSEALLQRRTGQADLLLVRNVDAGYGTRQILFDVDIDVREGEIVALLGT
                     NGAGKSTLLKAISGIVEADRGAIVLDGRDITHAPPNEIAVLGITQMPGGHGVFGGLTI
                     RENLQLAGWTQRRKPDEVARSTDEVLEMFPILREKFDAPAADMSGGQQQMLALAMAFI
                     AKPRVLLIDELSLGLAPVIVGQMLPIVQRLAADGVAIVMVEQSVNVALTVAERAYFLE
                     RGRVQFSGPTAELLARPDLLRSVFLSNASVGTPAASERPAREPGSGGADDAPPALSVN
                     GLVRSFGGIRAVDGVSFDVAPGEIVGIIGPNGAGKTTLFNLISGFIPTEAGRVMLADV
                     ELSRLSPPRRSAAGLGRSFQDAKLFPDITVAETLAVATERFVGTRSVLAAALRLPNSY
                     DSEQQVAARVDELIELMGLGRYRNSFIRELSTGTRRVVDLACQVAHRPTVILLDEPSS
                     GIAQREVEALAPVLLRLRDEMGAALVVIEHDMPLIS"
     assembly_gap    2678..2693
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2866..>4484
                     /locus_tag="PLV68_06645"
     CDS             2866..>4484
                     /locus_tag="PLV68_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00960.1"
                     /translation="MQPAQAGGGPNKSALKRWGPIAAIVAVIAVVIVVIVAVSGGDDK
                     TADSPTTTAAEVTTTTAATTTTAATTTTAATSPSNPTATDATDPAPAPVEITYPLSFS
                     QADAQGIDVDWGDRCDAERGTIAVPDFFAAECYAPFDGDNGGATDQGVTADEIVIAVY
                     QAVPNDPIIAYITDAIAVKDTNAQQADSMQNMVDYFQTYYETYGRTVRLEFVQGTGIA
                     SDETAARADAVHIAEDIKPFMVWGGPSLTSAFADELAARGVSCLSCTPAQNPSWYVER
                     DPLVWGITNSSDQGQTHVLEMIEKMLVGKNATHAGDDYKDKPRTFGLLYIESSAASKQ
                     LADDFEAGMVAAGAPLTERVAYLLDPGTIQQSASQAIAKFKAAGVTTVIFNGDPVAPR
                     EFTKEATAQGYFPEWFIGTSALVDTNAFARTYDQQQWAHAFGYTYLAAKTDSSKSGYY
                     FLHTWFTGEEPQAKDTINVFAPYPAVFFAALQGVGPNLTHETWGAALFAGGATRHALT
                     QPSLSWGEHGIWPYKDFVGIDDATMFWWDPTT"
     assembly_gap    4485..4512
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4738..5397)
                     /gene="ung"
                     /locus_tag="PLV68_06650"
     CDS             complement(4738..5397)
                     /gene="ung"
                     /locus_tag="PLV68_06650"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HRE00961.1"
                     /translation="MVTDWNPLLRGEFAKPYWQTLQQFVRDERTRATVYPPDDEVFAA
                     IHLTPHANTRVMILGQDPYHGPNQAHGLCFSVRRGVAIPPSLVNIYNELQSDLGIEPP
                     GHGNLTRWAEQGVLLLNTTLTVRAGQAASHQGKGWETFTDEVIRVVDAKTDPVVFILW
                     GSHARKKKALIDLSRHVVIESAHPSPLSAHNGFFGSRPFSRANAALIERGRDPIDWQL
                     D"
BASE COUNT          852 a         1889 c         1823 g          895 t
ORIGIN      
        1 cgtcgacgag ctcgaccggg ccgcgttcgg catcctgctg cccgagatcc gcgacgactt
       61 cggcatcgac ctcaagaccg cactcggcct ggtcgcgctt gcctcggtgg ccgcgctggc
      121 gctgcaggtg ccgatcgccc agatcgccga ccgttccaag cgggtgcgga tggcgatcat
      181 cggcgcgctg gtgtgggcga tgttcagcgg catgaccgga ctggcaaccg gcgtggtgat
      241 gttgacgatc gctcgctcgg ggtcggccgt cggcaaggcc gtcatcgacc cgacccacaa
      301 ctcgctgctc gccgactact acccgatcca cgtccgcagc aaggtgttca gcacgcaccg
      361 cgcggccaat gccgtcggcg cgttcgtcgg gccactcagc gcaggcctgt tggcctacgc
      421 gttcggttgg cgcactccct tcttcgtgtt catcgtgccg acggtgctgt tcgccgtgct
      481 cgccctgcgc atgcgcgagc cggtgcgcgg tcggtgggag cgggccgcgg ctggggccag
      541 tgacgacgtg gtcaacaccg aggagatccc ggccagtttt gccgagagct ggcgcacggt
      601 gcagaaggtg ccgagcctgc ggcggatgtg gtggtcgctg ccgttcctgg ccacgtcgct
      661 ggtcggcttc gtgaccttgg cgtcggtgat gtacgagcgc gagttcgacc tggacgagcg
      721 ggcccgtggt atcgccgcgg cgatcgccga gccgttcgcg ttggtcggca tcgtggtcgg
      781 cacgcgcatc atcccccgcc gtttcctggc cgacatgcag ggcctgatcc gtttcctggc
      841 cggttcggcg atcgtgtcgt cgttggcgtt cgcggcgttt gcgctgtcgc ccaacgtcgt
      901 cgtggccgtg gccatgaact gcgtcatctc ggcgatgttg gccacgatcg gcccgggcat
      961 cctggtcgcg ttctcgctgg cgatccccgc ccgtgctcgg gcaaccgggt tcagcatcgg
     1021 gtcgctgtgg gtgatccccg gactgttggt gctgccgctg gtgggctggg cggccgaccg
     1081 ctggaacgtg cgggttggga tgttggtgat gctgccgctg ttcgtgatcg gcgcgttcat
     1141 cctgcgcagc atgtcgacca cgatcaacgc cgacatcgcc caggtgtgga aggcaacagc
     1201 agcccgctcc gaggcgctgc tgcaacgccg caccggtcag gccgacctgc tgctggttcg
     1261 caacgtcgac gccggctacg gcacccgcca gatcttgttc gacgtcgaca tcgacgtgcg
     1321 cgagggcgag atcgtcgccc tgctcggcac caacggcgct ggcaagtcga cactgctcaa
     1381 ggcgatcagc ggcatcgtcg aagccgaccg tggcgcgatc gtgctcgacg gacgcgacat
     1441 cacccacgcc ccgcccaacg agatcgcggt gctgggcatc acccagatgc ccggcggcca
     1501 cggcgtgttc ggcggcttga ccattcgcga gaacctccaa ctcgcgggct ggacccagcg
     1561 gcgcaagccc gacgaggttg cccgcagcac cgacgaggtg ctggagatgt tcccgatcct
     1621 gcgcgagaag ttcgacgcac ccgcggccga catgagcggc gggcagcagc agatgttggc
     1681 cttggcgatg gcgttcatcg ccaagccgcg ggtactcctg atcgacgagc tgtcgctcgg
     1741 cctggctccg gtgatcgtcg gccagatgct gccgatcgtc cagcgtctcg ccgccgacgg
     1801 tgtggcgatc gtgatggtcg aacagagcgt caacgtcgcg ctcaccgtgg ccgagcgggc
     1861 ctacttcctc gaacggggcc gggtgcagtt cagcgggccg accgccgaac tcctggcccg
     1921 gcccgacctc ttgcgctcgg tgttcctgtc caacgccagc gtcggtacac cggctgcatc
     1981 cgagcggccc gctcgcgagc ccggcagcgg tggtgccgac gatgcgccac cggcgctgag
     2041 cgtcaacggc ctggtgcgca gcttcggcgg catccgtgcc gtcgacgggg tgagcttcga
     2101 cgtcgcgccg ggcgagatcg tcggcatcat cggacccaac ggcgcgggca agaccacgct
     2161 gttcaacctg atctcggggt tcatccccac cgaggccggg cgggtcatgc tcgccgacgt
     2221 cgagctgtca cggctcagcc cgccgcgccg ttcggcggcg gggctcgggc gtagcttcca
     2281 ggacgccaag ctgttccccg acatcaccgt ggccgagacg ttggccgtgg cgaccgagcg
     2341 attcgtcggc acccgcagcg tgttggccgc ggcgctgcgc ctacccaact cgtacgactc
     2401 cgagcaacag gttgcggctc gcgtcgacga gttgatcgag ctgatgggcc tgggccgcta
     2461 ccgcaactcg ttcatccgcg agctgtccac cggtacccgc cgcgtggtcg acctggcctg
     2521 ccaggtggcc catcgtccga cagtgatcct gttggacgag ccgtccagcg gcatcgccca
     2581 gcgcgaggtc gaggcgctgg cgcccgtgct gttgcgcctg cgcgacgaga tgggcgcggc
     2641 gctggtggtg atcgaacacg acatgccgtt gatcagcnnn nnnnnnnnnn nnnccaaggc
     2701 gcggtgctca ccaccggtct gccggccgac gtcctcgccc atcccgaggt ggttgccagc
     2761 tacctcggca cgtccgccgc ggcgatcagc cgctccggca aatagtcatc tgtccacgtc
     2821 acatccagcc cgatccaacc agcccgatcc aacaaggggg acatcatgca accagcacaa
     2881 gcagggggcg gaccgaacaa gtcggccctc aagcgatggg ggccgatcgc tgcgatcgtc
     2941 gccgtgatcg cggtcgtgat cgtcgtcatc gtcgccgtca gcggcggcga cgacaagacg
     3001 gccgactcac cgacgaccac cgccgcggaa gtcaccacga ccaccgccgc cacaacgacc
     3061 accgcggcca ccaccaccac ggcggccacg tcgccgagca acccgaccgc gaccgacgcg
     3121 accgatcccg cgccggcccc ggtcgagatc acgtacccgc tgtcgttctc gcaggccgac
     3181 gcgcagggca tcgacgtcga ttggggcgat cggtgcgacg ccgagcgcgg cacgatcgcc
     3241 gtgcccgact tcttcgctgc cgagtgctac gccccgttcg atggtgacaa cggtggtgcg
     3301 accgaccagg gcgtcaccgc cgacgagatc gtgatcgcgg tctaccaggc tgtgcccaac
     3361 gacccgatca tcgcctacat caccgacgcg atcgcggtga aggacaccaa cgctcagcaa
     3421 gccgattcga tgcagaacat ggtcgactac ttccagacct actacgagac ctacggccgc
     3481 acggtgcggt tggagttcgt gcagggcacc ggcatcgcgt ccgacgagac cgcagcccgt
     3541 gccgacgccg tccacatcgc cgaggacatc aagccgttca tggtgtgggg tggcccgtcg
     3601 ctgaccagcg cgttcgccga cgagctggcc gctcgcggcg tgtcctgcct gtcgtgtaca
     3661 cccgctcaga acccgtcgtg gtacgtcgag cgcgacccgc tggtgtgggg catcaccaac
     3721 agctccgatc agggtcagac ccacgtgctg gagatgatcg agaagatgct ggtcggcaag
     3781 aacgccaccc acgctggtga cgactacaag gacaagccgc gcacgttcgg tctgctctac
     3841 atcgagtcga gcgcggcctc caagcagttg gccgacgact tcgaagcggg catggtcgcc
     3901 gcgggtgcgc cgctgaccga gcgggtcgcg tacctgctcg acccgggcac gatccagcag
     3961 tccgccagcc aggcgatcgc caagttcaaa gccgccgggg tcaccacggt gatcttcaac
     4021 ggcgacccgg tggcaccgcg cgagttcacc aaggaagcga ccgcccaggg ctacttcccc
     4081 gagtggttca tcggcacctc ggcgctggtc gacaccaatg ccttcgcccg cacctacgac
     4141 cagcagcagt gggcccacgc gttcggctac acctacctgg cggccaagac cgactcgtcg
     4201 aagagcggct actacttcct gcacacctgg ttcacgggtg aagagccgca ggccaaggac
     4261 acgatcaacg tgttcgcccc gtacccggcg gtgttcttcg ctgccctgca aggggtgggc
     4321 ccgaacctca cccacgagac gtggggtgcg gcgctgttcg caggcggcgc gacccgccac
     4381 gcgctgaccc agccgtcgct gagctggggt gagcacggca tctggccgta caaggacttc
     4441 gtcggcatcg acgacgcgac gatgttctgg tgggacccga ccacnnnnnn nnnnnnnnnn
     4501 nnnnnnnnnn nncacgggca tgtaccagtt cgtcgacggc ggcacccgtt acctgccggg
     4561 cgagtggccg accgacgaca aattcttcga ccctgacggc gcggtggcgt tctactacga
     4621 cccgcccgcg tccgaagtcg cgccgcagta ccccagccca gcaggctgac cgaaccatca
     4681 ccctctgcgg aagccggatg gaccacgcgg tgcatccggc ttccgcggcg aggctgatca
     4741 gtcgagttgc cagtcgatgg ggtcgcggcc gcgttcgatc agggcggcgt tggcgcggct
     4801 gaacggacgg ctaccgaaga agccgttgtg cgccgacagc ggtgacgggt gggccgactc
     4861 gatcacgacg tggcgggaca gatcgatcag cgccttcttc ttgcgggcat ggctacccca
     4921 caggatgaac acgaccgggt cggtcttggc gtcgacgaca cggatcacct cgtcggtgaa
     4981 cgtctcccat cccttgccct gatgactggc ggcctgcccg gcgcgcacgg tgagggtggt
     5041 gttcaacagc agcacacctt gctcggccca ccgcgtgagg ttgccgtgcc ccggcggctc
     5101 gatgccgagg tcgctctgga gctcgttgta gatgttgacc agcgacggag ggatggccac
     5161 gccgcgccgt accgaaaagc acagcccgtg ggcctggttg ggaccgtggt acggatcttg
     5221 gccgaggatc atgacccgcg tgttcgcatg cggcgtcaga tggatggcgg cgaacacctc
     5281 gtcgtcgggt gggtacacgg tggctcgcgt ccgttcgtcg cggacgaact gctgcagggt
     5341 ctgccagtac ggcttggcga actcgccacg cagcaacgga ttccaatcgg tgaccattgc
     5401 aagtcagtga tatcacgccg cgcggtaccc accgccaacg gcgcgcatct acagtcggac
     5461 acgtgccgct cggtcgtctc gccttgtttc ctcgcgtgcc cct
//
LOCUS       DAORNF010000131         5427 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_11175_length_5427_cov_1.690618, whole genome shotgun sequence.
ACCESSION   DAORNF010000131 DAORNF010000000
VERSION     DAORNF010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 5427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5427
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11175_length_5427_cov_1.690618"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            75..851
                     /locus_tag="PLV68_06655"
     CDS             75..851
                     /locus_tag="PLV68_06655"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HRE00962.1"
                     /translation="MSIVFFHAHPDDESIFTGGTIARLSAAGHRCVIVIATSGELGTP
                     AGGHEAGDDAADEGWELATIRRREAHRAAERLGAARIDFLGYSDSGLTAMGEPPVGAF
                     AVANVAEAASRLAQILVEEAASELVIYDDGGIYGHPDHLAVHRVGLAAAAMSSTETVY
                     EATVDREYLHFVETHLVGHALDALGEPAPMGVPTVLLSATVDVRAVIDAKREAMAAHV
                     SQLPPHAPVLTMDRETFAAVYGFEWFVRHGPAGLIDTLGF"
     gene            879..1691
                     /locus_tag="PLV68_06660"
     CDS             879..1691
                     /locus_tag="PLV68_06660"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HRE00963.1"
                     /translation="MSSHDPSSRDDVKYHIGFARRDLPEGTSIALLSGDPGRSELIAQ
                     QYLANGRELARNRGLDSFVAELPGGAPVVCATSGMGAPSMSIVVNELAAIGITTVIRI
                     GTSGSIQDHVRVGEVVIASGALTNQGAALDIAPVEFPAAADPFLVVQLAQAAAAIGAA
                     HHVGVMASVDTFFEGQERTATSANPHLIRRLRGMIDEYHDLGVLNFEMEAGTLLKMGL
                     VYGFAAGCVCAIVAQRTEDENPRLDLKDAAIDNAIRVAIAGADTRSLSATSG"
     gene            complement(1672..2433)
                     /locus_tag="PLV68_06665"
     CDS             complement(1672..2433)
                     /locus_tag="PLV68_06665"
                     /inference="COORDINATES: protein motif:HMM:NF016211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HRE00964.1"
                     /translation="MADDDERTARNPIARLLWTVGGFVLIGIGLIGIVVPGLPTTGFL
                     VAAAACFSRSSPRFERWVLNLPTFGPMVRDYRAGLGMPRRAKVAACTSIVVACSLSSF
                     LAVERWWVRIAILVLGAIGVAWILFRIPTRAEPTPAELAAREQAEYRRGARAAMSRPS
                     RIFRIVAFVETLTWVGLLAGMAQKYLFDGGERGVALFGPIHGTVVLVYFAALIWAGTA
                     NRWNARTFILGALASVPPFASLQFERWAEVNRKLR"
     gene            complement(2490..>3179)
                     /locus_tag="PLV68_06670"
     CDS             complement(2490..>3179)
                     /locus_tag="PLV68_06670"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HRE00965.1"
                     /translation="YAALAAYGISAVTGEVVGREHAGGDEPDGHRPDNDAVIADALIA
                     AARRIGYPVVIKAAHRRVGRSAQAGIALDLANDDDVRTAVATIRASLGPQTQAFTVQA
                     MTAPGIDVRIRCATDDQLGPVITLDLGSLQEIDPTSGVSRLPPISRVTAQAMVDASRV
                     GAALTAAGIDAAPVVDAVMRVGQLMFDHSEIRSIEINPAIATSTACVVTDVTIVADDE
                     RPSDFPIRRLG"
     assembly_gap    3180..3206
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3207..>5123)
                     /locus_tag="PLV68_06675"
     CDS             complement(<3207..>5123)
                     /locus_tag="PLV68_06675"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRE00966.1"
                     /translation="MDERDPDADPSAARTHDDDRDRWSAAVVLSDGDTAFIRPITTAD
                     KDALAELHVAQPAENLYRRFFSPKPTLSDAELEHFTNIDFRDRVALAVERRGKWIAWA
                     SYERWRDRDDAEVAFLVDAGHQGLGIATILLEHLASIARSNGITRFTAETLADNRAMV
                     TVFNRAGWPVEKHFDSGLTEVEFPLVETESFVDSVEVREQRADSRAVARLLLPKSIAV
                     IGASDQPGSIGRELWNNALRFAGPVYPVNHRHDTVGGRPAYASITDIADDVSLALVAV
                     PAAALAATIDQCIAKRVRGAVVVTAIDGAGIDLTALVAHARRNGLRIIGPASMGVATT
                     RPGGLKGALVPLELPPGNVAISMQSGTLGASLLQLALQMHMGVSWFVSLGDKSDVSGN
                     DLLQFWEDDESTSVIAMYTESFGNPRKFARIARRVGRRKPIVAVRTGTAAVGNAATAL
                     YRQAGLVEVPTVRSMLDTARCFATQPIPRGPNVAILTNARSPGVLAAAAIVAAGLQVV
                     DAPVELGWRSTSTDYGDAIAAALAADHVDAVLVIHAPPVIASPAPLAAIDSATAGATK
                     PVLAVMLGRNDGPLSARSAVPSFSFPEAAAASLGRMYAHGRWLATEAEPGSEAIDVSA
                     DTTGVIIAEALAGRD"
     assembly_gap    5124..5182
                     /estimated_length=59
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5183..>5427
                     /locus_tag="PLV68_06680"
     CDS             <5183..>5427
                     /locus_tag="PLV68_06680"
                     /inference="COORDINATES: protein motif:HMM:NF019633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="siderophore-interacting protein"
                     /protein_id="HRE00967.1"
                     /translation="TDDSTATKSLEQHRAEMDAIPTLFTQVVRVEALTPHLKRITFGG
                     GDLVEFVPKSPDQFMYVLLPPPGQSETKPGRFSFSVP"
BASE COUNT          834 a         1811 c         1884 g          812 t
ORIGIN      
        1 ccctacccaa tcgatcccga ggtcagtcgg cccgtatcga tgccgtgtgc cagactcccc
       61 gtcgtgatca gggcatgagc atcgtcttct tccatgccca ccccgacgac gagtcgatct
      121 tcaccggtgg caccatcgcc aggctgtccg ccgcgggtca tcgatgcgtg atcgtcatcg
      181 ccacatcggg tgaactcggc acaccggccg gtgggcacga agcgggcgat gacgccgcag
      241 atgaggggtg ggagctggcc acgatccgcc gccgcgaggc ccaccgcgcc gcggagcggc
      301 tcggggcagc ccggatcgac ttcctcggct actccgacag cgggttgacg gcgatgggcg
      361 agccgccagt gggagcgttc gctgtggcca acgtggccga agccgcgtcc cgcctggccc
      421 agatcctggt cgaggaagcg gccagcgagc tcgtgatcta cgacgacggt ggcatctacg
      481 gtcaccccga ccaccttgcg gtccaccggg tcgggctggc cgcggcggcg atgtcgtcga
      541 ccgagacggt ctacgaggca acggtcgacc gtgagtacct tcacttcgtc gagacccacc
      601 tggtcggcca cgcgctcgat gcgctgggcg aaccggcgcc gatgggtgtg ccgacggtgc
      661 tgctgtcggc cacggtcgac gtgcgcgcgg tgatcgacgc caagcgcgag gcgatggctg
      721 cccacgtcag ccaactgcct ccgcacgctc cggtgctgac gatggaccgg gagaccttcg
      781 ccgcggtcta cggcttcgaa tggttcgttc gccacggtcc ggccgggttg atcgacacgc
      841 tgggcttctg acctgacagc gccactacag tcgggcgcgt gtccagtcat gatccgtcca
      901 gccgtgatga cgtcaagtac cacatcgggt tcgctcgccg cgacctgccc gagggtacgt
      961 cgatcgccct gctcagtggc gatccgggcc ggtcggagct gatcgctcag caatacctgg
     1021 cgaacggtcg tgagctggcc cgcaaccgtg ggctcgacag cttcgtggcc gagttgcccg
     1081 gcggtgcacc ggtggtgtgc gccaccagcg gcatgggtgc gccgtcgatg agcatcgtcg
     1141 tcaacgagct ggctgcgatc ggtatcacga cggtgatccg catcggcacg tccgggtcga
     1201 tccaggacca cgtccgtgtc ggcgaggtgg tcatcgcgtc gggcgcgctg accaaccagg
     1261 gtgccgccct cgacatcgcc ccggttgaat tcccggccgc ggccgacccg ttcctggtgg
     1321 tgcagctggc ccaggcggcg gcggcgatcg gtgctgctca ccacgtcggg gtgatggcct
     1381 cggtcgacac gttcttcgaa ggccaggagc gcacggccac ctccgccaat ccgcatctca
     1441 tccggcgcct gcgcggcatg atcgacgagt accacgacct gggggtgctc aacttcgaga
     1501 tggaagccgg cacgctgttg aagatggggt tggtctacgg cttcgcggcc ggatgcgtgt
     1561 gcgccatcgt cgcccagcgc accgaggacg agaacccgcg cctcgacctg aaggacgcgg
     1621 cgatcgacaa cgcgatccgg gtcgccatcg cgggggccga cacccgctcg ctcagcgcaa
     1681 cttccggttg acctcggccc accgttcgaa ctgcagcgag gcaaagggcg gcaccgacgc
     1741 cagcgcgccg aggatgaacg tgcgcgcgtt ccagcggttg gcagtaccgg cccagatcag
     1801 cgcggcgaag tacaccagca ccaccgtgcc gtggatcggc ccgaacagcg cgaccccgcg
     1861 ctcgccgccg tcgaacaggt acttctgagc catgcctgcc agcaggccca cccatgtcag
     1921 cgtttcgacg aaggcgacga tgcggaagat tcggctcgga cggctcatcg cggctcgtgc
     1981 cccacggcga tactcggcct gctcgcgggc ggccagttcg gccggggtcg gctcggcgcg
     2041 ggtggggatg cggaacagga tccacgcgac gccgatcgcg cccagtacca ggatcgcgat
     2101 gcgcacccac cagcgctcga ccgcgaggaa cgaactgagc gagcaggcga ccacgatcga
     2161 ggtgcacgcc gcgaccttcg ccctgcgcgg catgcccagc ccggcgcggt agtcgcgcac
     2221 catcggcccg aacgtcggca ggttcagcac ccaccgctcg aagcgggggc tcgatcgcga
     2281 gaagcaggcc gcagcagcca ccagaaagcc ggtcgtcggc aggcccggca cgacgatgcc
     2341 gatcaacccg atgccgatca gcacgaatcc gcctacggtc cacaacagcc gagcgatcgg
     2401 attgcgcgcc gtgcgctcgt cgtcgtcagc catgcgtgac cgaggctagt gggtgtcaag
     2461 cacccttcac cgggctggct gctcaggcat cacccgagcc ggcgtatcgg gaagtcactg
     2521 gggcgttcgt cgtcggccac gatcgtgacg tcggtgacca cgcatgccgt gctggtcgcg
     2581 atggccgggt tgatctcgat cgagcggatc tcactgtggt cgaacatcaa ctggccgaca
     2641 cgcatcaccg cgtcgaccac cggtgccgcg tcgatgccag ccgcggtcag cgccgcgccg
     2701 actcgcgacg catcgaccat cgcctgggca gtgacgcgtg agatcggtgg cagacggctg
     2761 accccggaag tggggtcgat ctcctgcagg ctgcccaggt cgagggtgat caccggaccg
     2821 agttggtcgt cggtggcgca gcggatgcgc acgtcgatgc ctggcgcggt catcgcctgc
     2881 acggtgaacg cctgcgtctg cgggccgagc gaggcgcgga tcgtggcgac cgcggtccgc
     2941 acgtcgtcgt cgttggcaag gtcgagggcg atgcctgcct gagccgagcg cccgacccgg
     3001 cgatgcgcgg ccttgatcac caccggataa ccgatccgcc gcgcggcggc gatcagcgca
     3061 tcggcgatca ccgcgtcgtt gtcgggccgg tggccgtccg gctcgtcgcc accggcatgc
     3121 tcacggccga ccacttcacc ggtgaccgcg ctgatgccgt aggcggcgag cgcggcatan
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnatcg cgcccggcga gcgcttcggc gatgatcacc
     3241 ccggtcgtgt cggcgctgac gtcgatcgct tcggatccgg gctcggcctc ggtggcgagc
     3301 caccggccgt gggcgtacat ccgccccagg ctggctgccg cggcttcggg gaacgagaac
     3361 gacggcactg ccgagcgtgc cgacagcggg ccgtcgttgc gccccagcat cacggccagc
     3421 accggcttgg tcgcgccggc cgtggccgag tcgatggccg cgagcggggc tggggaggcg
     3481 atcaccggtg gggcgtggat caccagaacg gcatcgacgt gatcggcagc gagcgcggcc
     3541 gcgatggcat cgccatagtc ggtagaggtc gatcgccagc cgagctcgac cggcgcgtcc
     3601 acgacctgca gcccagccgc gacgatcgct gcggcggcaa gcacaccggg gctgcgggcg
     3661 ttggtgagta tggcgacgtt cggtccgcgg gggatcggtt gggtcgcgaa gcagcgcgcc
     3721 gtgtcgagca tggagcgcac cgtcggcacc tcgaccagac cggcctggcg gtacagcgcg
     3781 gtagcggcgt tgccgacggc agcggtgccg gtgcggaccg cgacgatcgg cttgcggcgc
     3841 cccacccgcc gggcgatgcg agcgaacttg cgcgggttgc cgaacgactc ggtgtacatc
     3901 gcgatcacac tggtgctctc gtcgtcctcc cagaactgaa gcaggtcgtt gcccgatacg
     3961 tcggacttgt cgcccagcga gacgaaccac gacacgccca tgtgcatctg caacgccagc
     4021 tgcagcaacg aggcaccgag ggtgcctgac tgcatcgaga tcgccacgtt gcctgggggg
     4081 agctcgagtg gcaccagcgc gcccttcagc ccgcctgggc gtgtcgtggc cacacccatg
     4141 ctcgccggac cgatgatgcg cagcccgttg cggcgagcgt gagcgaccag ggcggtcagg
     4201 tcgatgccag cgccgtcgat cgccgtgacc acgactgcgc cacggacgcg cttggcgatg
     4261 cactggtcga tcgtggcggc caacgctgcc gcgggaacgg ccacgagtgc cagcgagacg
     4321 tcgtcggcga tgtcggtgat cgacgcgtac gccggccgac cgccgacggt gtcgtggcgg
     4381 tggttgaccg gatagacggg accggcgaag cgcagcgcgt tgttccacag ctcgcgcccg
     4441 atcgagccgg gttggtcgga tgcgccgatc acggcgatcg atttgggcag caggagacga
     4501 gccaccgccc ggctgtcggc acgctgttcg cggacctcca ccgaatcgac gaacgactcg
     4561 gtctcgacca gcgggaactc cacctcggtc agcccgctgt cgaagtgctt ctccaccggc
     4621 cacccggccc ggttgaacac ggtgaccatc gcccggttgt cggccagcgt ctcggcggtg
     4681 aagcgggtga tgccgttgga gcgggcgatc gaggccaagt gttcgagcag gatcgtggcg
     4741 atgccgagac cctggtgccc ggcgtcgacc aggaacgcca cctcggcgtc gtcgcggtca
     4801 cgccagcgtt cgtagctggc ccacgcgatc cacttgccgc gccgttcgac ggccaaggcg
     4861 acccggtcgc ggaagtcgat gttggtgaag tgctccagtt cggcgtcgct caaggtcggc
     4921 ttgggcgaga agaagcggcg gtacaggttt tcggcgggct gggcgacgtg gagctcggcg
     4981 agcgcgtcct tgtcggccgt ggtgatcgga cggatgaagg cggtgtcgcc gtcggacagc
     5041 accacggcgg ccgaccagcg gtctcgatcg tcgtcgtggg ttcgggcggc actcgggtcc
     5101 gcgtcggggt cgcgctcgtc catnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5161 nnnnnnnnnn nnnnnnnnnn nngaccgacg acagcacagc cacgaagagt ctcgaacagc
     5221 accgcgccga gatggatgcg atccccaccc tgttcaccca ggtcgtgcgg gtcgaggcgt
     5281 tgaccccgca tctcaagcgg atcaccttcg gcggtggcga tctggtcgag ttcgtgccga
     5341 agtcgcccga ccagttcatg tacgtgttgt tgccgccacc ggggcagagc gagacgaaac
     5401 cgggcaggtt ttcattctcg gtgccga
//
LOCUS       DAORNF010000132         5362 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_11356_length_5362_cov_1.434897, whole genome shotgun sequence.
ACCESSION   DAORNF010000132 DAORNF010000000
VERSION     DAORNF010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 5362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5362
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11356_length_5362_cov_1.434897"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>634
                     /locus_tag="PLV68_06685"
     CDS             <1..>634
                     /locus_tag="PLV68_06685"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HRE00968.1"
                     /translation="IAAFLAVLAVGIGAYLVFQSLNKDEPVTKFALDDYVGMTLEEAS
                     QRLLDLDLVPKGVKREDTNQAPGLVFETDPKAGVIITKGMTITLYYRPEDELRAVPDV
                     RNKTLAEAKELLLVAGFEVADPVNEVVDPSVPVGSVISQDPAPKAMEKQGTAITLTVS
                     QGPGKVTIPNVAEQMADAATDVLRSAAFNFSVNRVQESNDAVPAGRVIRTD"
     assembly_gap    635..678
                     /estimated_length=44
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <679..1054
                     /locus_tag="PLV68_06690"
     CDS             <679..1054
                     /locus_tag="PLV68_06690"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HRE00969.1"
                     /translation="TIVVSSGPVQVSVPFVVGQTESKAIEKLAEVNLLARVSYRDVPF
                     GHPNDKVVLEQNPGVGQQVNSSSYVELVVGRALPQPTTTPPTVPPTLAPTTTVAPTTT
                     VAPTTTAPPPTTTTTTETTPDP"
     gene            complement(1094..>1626)
                     /locus_tag="PLV68_06695"
     CDS             complement(1094..>1626)
                     /locus_tag="PLV68_06695"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HRE00970.1"
                     /translation="VQYLGELGADPVVFRNDALTVDEAVALAPDAVLLSPGPGRPEEA
                     GILCAAVTAFAAIGTPVLGVCLGHQGIGHVYGAAIVRAHELMHGKTSVIHHDETGVFR
                     GLDNPLQATRYHSLVIDPDTIPDCLEVTARTDDGLVMGVRHRELRVEGVQFHPESILT
                     TGGHQLLRNFLDSSAA"
     assembly_gap    1627..1636
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1808..2140
                     /locus_tag="PLV68_06700"
     CDS             1808..2140
                     /locus_tag="PLV68_06700"
                     /inference="COORDINATES: protein motif:HMM:NF018487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CrgA"
                     /protein_id="HRE00971.1"
                     /translation="MTPPKRKTGGGRVTPKGTKPGQVAATPSSLAHNAHTRAGEVAAS
                     SRYTPPVPLSVKESPTWVPVLMVALLIIGALTIIARYLLNDSVGNWLVFVGLGFVLAG
                     LFTATKWH"
     assembly_gap    2421..2442
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2588..>2852)
                     /locus_tag="PLV68_06705"
     CDS             complement(2588..>2852)
                     /locus_tag="PLV68_06705"
                     /inference="COORDINATES: protein motif:HMM:NF024988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HRE00972.1"
                     /translation="LADRLAASPASISRQLNSLADAGYVRRERPTNMDNRAVVVVLTK
                     EGREAWRTANTVYLRVVKKHFLSHLGDADVAAMRQAFDGILAE"
     assembly_gap    2853..2915
                     /estimated_length=63
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3145..3627)
                     /locus_tag="PLV68_06710"
     CDS             complement(3145..3627)
                     /locus_tag="PLV68_06710"
                     /inference="COORDINATES: protein motif:HMM:NF026109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase C-terminal domain-containing
                     protein"
                     /protein_id="HRE00973.1"
                     /translation="MQRDGVVCDASLRALGTDGAAIPGVYAAGDIARWPNGAFGGEEM
                     RVEHWTTTSEQARHVAEALIADSAGQPVEPFVTVPFFWSDQYDRRIQFLGRAGGDDEV
                     RVVAGSVESRSFVALYGRDGRVRGALGVSMPKQLMRARALVAEGVAFDEALAAAAAWS
                     "
     assembly_gap    3757..3766
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3767..>4273)
                     /locus_tag="PLV68_06715"
     CDS             complement(<3767..>4273)
                     /locus_tag="PLV68_06715"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRE00974.1"
                     /translation="EALAIDLRLGVAATGLDLGLDRSREPGEAASRRVLLGDGTAVAC
                     DAVVVATGAGLRHLPGQPDHPAITELRTLDDSLALQQRIAGGGGHVVVIGAGFIGLEV
                     AASARALGNEVTVLEGAPAPLIRGLGAEMGQVIAAVHADHGVPVHCGVRVGDLTPRAD
                     GSVEVAVL"
     assembly_gap    4274..4382
                     /estimated_length=109
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4660..4821
                     /estimated_length=162
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4822..>5362
                     /locus_tag="PLV68_06720"
     CDS             <4822..>5362
                     /locus_tag="PLV68_06720"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HRE00975.1"
                     /translation="GVIGFELHRGAVAAADRADLHTLPDVLATSRTIAVLEGLNDTEN
                     LGAIARSARALGIDALVLDPTCADPFYRRAVRVSMGEILFLPVVRAVAWPGALDAIAA
                     AGFRLLSLTPDPTATPIDAVDRRDDDRIALLLGAEGPGLTASTLARTEAVRIPQRPDV
                     DSINVGHAAAIAFSRVGRL"
BASE COUNT          864 a         1798 c         1569 g          711 t
ORIGIN      
        1 catcgccgcg ttcctggccg tgctcgccgt gggtatcggc gcatacctgg tgttccagtc
       61 gctcaacaag gacgagccgg tcaccaagtt cgcgctcgac gactacgtcg gcatgacgct
      121 ggaggaggcc agccagcgac tgctcgacct cgacctcgtc cccaagggcg tcaagcggga
      181 agacaccaac caggcgcccg gcttggtgtt cgagaccgac ccgaaggccg gggtcatcat
      241 caccaagggc atgacgatca cgctctacta ccgcccggaa gacgagctgc gagccgttcc
      301 cgacgtgcgc aacaagaccc ttgccgaggc caaggagctg ctgctcgtcg ccgggttcga
      361 agtcgccgat ccggtcaacg aagtggtcga tccgtcggtg ccggtcggct cggtcatcag
      421 ccaggacccc gcgccgaagg cgatggagaa gcagggcacc gcgatcacgc tcaccgtgtc
      481 gcaggggccg ggcaaggtca cgatccccaa cgtggccgag cagatggccg acgccgcgac
      541 cgacgtgttg cgctcggccg cgttcaactt ctccgtcaac cgggtgcagg aatcgaacga
      601 cgccgtgcca gccggccggg tgatccgcac cgacnnnnnn nnnnnnnnnn nnnnnnnnnn
      661 nnnnnnnnnn nnnnnnnnga ccatcgtggt cagctccggg ccggtgcagg tgtcggtgcc
      721 gttcgtggtc ggccagaccg aatcgaaggc gatcgagaag ctggccgagg tcaacctgtt
      781 ggcccgcgtc agctatcgcg acgttccctt cggccatccc aacgacaagg tggtgctcga
      841 acagaacccg ggtgtcggcc aacaggtcaa ctcgagctcg tacgtcgaac tggtcgtcgg
      901 gcgggcactg ccccagccga ccaccacccc accgacggtg ccgcccacgc tggcgcccac
      961 caccacggtc gcgccgacca ccacggtcgc ccccaccacc acggcgccgc cacccaccac
     1021 gaccaccacc accgagacca cgcccgatcc gtaaccggcc ggacacgtcc ggcggccgca
     1081 gcgggtgtgc gcgtcaggcc gcgctggagt cgaggaagtt gcgcagcagc tggtgaccgc
     1141 ccgtggtgag gatgctctcc gggtggaact gcaccccctc gacgcgcaac tcacggtggc
     1201 gaacccccat caccaacccg tcgtcggtgc gagccgtcac ttcgaggcag tcggggatcg
     1261 tgtcagggtc gatcaccagc gagtggtagc gcgtggcctg cagcgggttg tcgagcccac
     1321 ggaacacgcc ggtctcgtcg tggtggatga cgctggtctt gccgtgcatc agctcgtgcg
     1381 cgcgcacgat cgcggcaccg tagacgtggc cgatgccctg gtggcccagg cacacaccca
     1441 acaccggcgt accgatggcc gcgaacgcgg tgaccgccgc gcacaagatg cccgcctctt
     1501 cgggacgacc cggcccgggc gacagcagca ccgcgtcggg cgccaacgcg accgcctcgt
     1561 cgacggtgag ggcatcgttg cggaagacga caggatcggc cccgagctct cccaagtact
     1621 gcaccannnn nnnnnngtcg tagctgtcga gcacgagcac tcggggggat gacgtcaccg
     1681 ctacagcctg ccatccgcga cccccggcgg ggcaacgaga tcaccgggag aacgagatcg
     1741 ggcggcacta cactccccac agcccgccgt ggcgggttgc agccgacgag cccagcagga
     1801 catctccatg acacctccca agcgcaagac cggtggcggc cgggtcacac ccaagggcac
     1861 caagccgggt caggtcgccg cgaccccgtc gtcgctcgct cacaacgccc acactcgagc
     1921 gggtgaagtg gccgcatcgt cgcgctacac cccacccgtg ccgctgagcg tcaaggagag
     1981 ccccacctgg gtgccggtgc tgatggtggc gctgctgatc atcggcgcgc tcacgatcat
     2041 cgcccgctac ctgctcaacg actcggtcgg caactggctg gtgttcgtcg gtctcgggtt
     2101 cgtgctggcc gggctgttca ccgcgaccaa atggcattga ccacgtcgca ttgaccacgt
     2161 cgcattgacc acgtcgcatt gaccacatgg cattgactgg cgccagcgac gcctctccgg
     2221 ccgaaatcac acgggtgtag ttcttcaaca cctttatcca cagaatgtgg acaaaggatc
     2281 agaagtcgat cggggccagc aggtccatgt cttcgaggca atgccggggt ggcggcgggg
     2341 tttcgtcggg ggccaaggtc atccgtaact ccaggccaca gactccacag cgataccgga
     2401 cattgatgcg acgcatctca nnnnnnnnnn nnnnnnnnnn nnagcggcgt cgtcatctga
     2461 cgcagcaggg tgatccctgt tttccacagg acccagccca acacgaaggc gccagcgacc
     2521 cacaggacgg tcgacaacgc aggcatgtgc ccaccctacc gacgcctgac gcgtggaccc
     2581 gaaacggcta ttcggcgagg atgccgtcga aggcctggcg catcgcggcc acgtcggcgt
     2641 cgccgagatg actcaggaag tgcttcttga ccacgcggag atagacggtg ttggcggtcc
     2701 gccacgcctc gcggccctcc ttggtgagca cgacgacgac ggcccggttg tccatgttcg
     2761 tcggtcgctc gcggcggaca taaccggcgt cggccaagct gttcaactgg cgcgagatgc
     2821 tggccggcga ggccgccagc cgatcggcaa gcnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngctcg tgatcgaggg ccacgaccag
     2941 acgtcgtcgc aactgcgcct gggcggtcac gaactggcgc cacacggtca ggcgttcggg
     3001 atcgatcgtg gccatctcgg tgctccgtta cgtcgagggc agtcgcgccg gctcccgccc
     3061 gaggtcgatg ccgccgatgg tacgccaccg cagccagccc gctgcgcttc gcgggctcgg
     3121 cctgatcgtg ccttggagac ctggtcagga ccacgctgca gcggcggcga gcgcttcgtc
     3181 gaaggccaca ccctcggcga ccagagcccg ggcgcgcatc aactgcttgg gcatcgacac
     3241 accgagcgcg ccgcgcacgc gtccgtcacg cccatagagc gcgacgaacg accggctctc
     3301 gaccgagccc gcgaccacac ggacctcgtc gtcgccaccg gcgcggccga gaaactggat
     3361 gcgccggtcg tactgatcgc tccagaagaa cggcaccgtc acgaacggtt caaccggctg
     3421 tccggccgag tcggcaatca gcgcctcggc cacatgcctg gcctgctcag aagtggtcgt
     3481 ccaatgctcg acccgcatct cttccccacc gaaggccccg ttcggccagc gagcgatgtc
     3541 accggcggca tatacccccg ggatcgccgc accgtccgtt cccagcgccc gcagggacgc
     3601 gtcgcagacg acaccgtcgc gctgcaacgc ggcctcgatg ccagctgcgg agaactcgct
     3661 gagtttcgca ataagtcagc tatcctattt attcccaacg gcacttatcg tgtaactcaa
     3721 actctggttg tgcgacattc gaaaggtcct tggatcnnnn nnnnnncaca gcacggcgac
     3781 ctcgacgctc ccgtccgcac ggggcgtgag gtcaccgacc cggacaccgc agtggaccgg
     3841 cacaccgtga tcggcgtgaa cggcggcgat gacctgaccc atttccgctc cgagcccacg
     3901 gatcagcggt gccggagcac cctccagcac cgtcacctcg ttgcccaacg cgcgggccga
     3961 ggccgcaacc tccaacccga tgaagccggc accgatcacc accacatggc caccgcctcc
     4021 cgcgatgcgc tgctgcagcg cgagcgagtc gtcgagggta cgcagttcgg tgatcgccgg
     4081 gtgatcaggt tggccgggca agtggcgcaa ccccgcaccg gtcgcgacga ccacggcgtc
     4141 gcaggcaacg gcggtgccat cgcccagcag cacccgacgc gacgccgcct cgccgggctc
     4201 gcgggagcga tcgaggccga gatcgagacc ggtcgcggca acgccgagac ggagatcgat
     4261 cgccagcgcc tccnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4381 nnaacacgat ctcgccctcg aacccgctgg cccgcagcgc accgcacaca cgcacgccgg
     4441 ccaacgaagc acccacgacg gcgacacgat cgaccctcat cggccggagc gtagttggca
     4501 cgttgtcatt cagccgctgg ccgcggtgaa ctggctacgt tgatcggggg atggaaccga
     4561 tcgcgatcac cagcgctgac gatgcgcgcg tcagcgacta cgccgatctg cgcgatccct
     4621 acgtgcggcg caagctggag ggtgatgagt acttcatcgn nnnnnnnnnn nnnnnnnnnn
     4681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4801 nnnnnnnnnn nnnnnnnnnn nccggggtca tcggcttcga actgcatcga ggcgcggtcg
     4861 cggcggccga tcgggccgac ctgcacaccc tgcccgacgt gctcgcgacc agccgtacga
     4921 tcgccgtgct cgagggcctc aacgacaccg agaacctggg cgcgatcgcc cgctcggcgc
     4981 gagccctcgg catcgacgcg ctggtgctcg accccacgtg cgccgacccc ttctaccgcc
     5041 gcgcggtgcg ggtgtccatg ggcgagatcc tgttcctgcc cgtcgtccgc gccgtcgcct
     5101 ggccgggcgc gctggacgcc atcgcggccg ccgggttccg gctgctgtcg ctgacccccg
     5161 acccgaccgc tacacccatc gacgccgtcg atcgccgcga cgacgatcgc atcgccctgt
     5221 tgctcggtgc cgaaggccct ggcctcacgg cctccaccct ggcgcgcacc gaagcggtcc
     5281 gcatcccgca acgtccggac gtcgactcca tcaacgtcgg tcacgccgcg gcgatcgcct
     5341 tcagccgcgt cggccgcctc ga
//
LOCUS       DAORNF010000133         5346 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_11402_length_5346_cov_1.536572, whole genome shotgun sequence.
ACCESSION   DAORNF010000133 DAORNF010000000
VERSION     DAORNF010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 5346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5346
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11402_length_5346_cov_1.536572"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..713
                     /gene="pstC"
                     /locus_tag="PLV68_06725"
     CDS             <1..713
                     /gene="pstC"
                     /locus_tag="PLV68_06725"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HRE00976.1"
                     /translation="LIAVVLAVPVSLGLALFTTQVAPKWLRRPLVTLTDIVAVVPSVV
                     FGLWGVIYLAPRLVGVFKTLNGWFDGVPVLSSIFGRVGGGRSFLTTGLILAVMITPII
                     TSIAREVIDTTPQADRDGALALGATRWEMITGAVLPHSAGGIVGGVMLGLGRAMGETI
                     AAALVIGSSPAITANIFSSGDSLPAVIAFQWGEATSDEKRSALIAMGLTLFVLTLLVN
                     FAATAIVNRAMKRMQGAS"
     gene            713..>933
                     /locus_tag="PLV68_06730"
     CDS             713..>933
                     /locus_tag="PLV68_06730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00977.1"
                     /translation="MTAASPTATIPSPLTRLSVTDLGGVRGRRRATNAVITVLMGTSV
                     VVIALVLLLVLWTVVSKGWSIVAGQFPAW"
     assembly_gap    934..950
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <951..1653
                     /gene="pstA"
                     /locus_tag="PLV68_06735"
     CDS             <951..1653
                     /gene="pstA"
                     /locus_tag="PLV68_06735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HRE00978.1"
                     /translation="IQLSARRPGPGMKAAIVGTIVTTVGASLIAIPLGILGAVYLNEY
                     GKRSVFARVLRFFTSVMAGVPSIVMGLFIYVFWVVPRGTAGLNSFSGALALACLMLPI
                     VVRSTEEMLRLVPQNLRDASLALGATGSRTILGIVIPKAAPGIISGCLLAVARAAGET
                     APLIFTVGAAKSTNWNLFDGTTTTLSLQIYANAKESFATAQERAWGSALTLIALAVLF
                     TVVARLMSARLTKSS"
     gene            complement(1684..>2471)
                     /locus_tag="PLV68_06740"
     CDS             complement(1684..>2471)
                     /locus_tag="PLV68_06740"
                     /inference="COORDINATES: protein motif:HMM:NF025224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HRE00979.1"
                     /translation="TWHGVTCEGGSIRTLLLQGNQLTGSIPNTLEDLVDLEYLILSDN
                     QLGGSIPAQLGNLDDLRGLQLGNNHLSGSIPAELGSLVNLRDLDLSGNRLTGAIPLQL
                     VNLTHLQNLYLTNNQLSGSVPVELGSLVNIKALSLGSNRLTGSIPTQLGNLSALQFLD
                     LGHNQLTGSIPAQLGKLANLQALWLHSNQLAGSIPSQVMSIQGLQSLLLQTNQLTGPI
                     STNIAHLNNLQELWLRGNGCLTAEIGELVAWLTAKDPLWNDGC"
     assembly_gap    2472..2639
                     /estimated_length=168
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2640..>2851)
                     /locus_tag="PLV68_06745"
     CDS             complement(<2640..>2851)
                     /locus_tag="PLV68_06745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00980.1"
                     /translation="MGVDISVLFGYLVGVYVEAGIELGLWVEVTDIDVCHRLGLDFVL
                     SAGVRLELWWKSYSYEFAETRAKLPG"
     assembly_gap    2852..3047
                     /estimated_length=196
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3048..4646)
                     /locus_tag="PLV68_06750"
     CDS             complement(<3048..4646)
                     /locus_tag="PLV68_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00981.1"
                     /translation="MVLNVTVDQPTVGSFLTVWPTGAPRPNASNLNYRADQTVPNLVT
                     AQLGLDGKVSIFNAAGSAHVIADVAGWFPAISGFAPMQPSRLADTRADGETVDGRGAR
                     SGALGSGTVLDVQVTGRARVPADATAVVVNVTAVTPTTASYLTVWPTGAPRPWASNLN
                     LSPGAVRPNLVVARVGEGGQISVFNASGAVDVVVDVAGWFTLNSGYFALTPARLADTR
                     ADGATIDGLAARSGALAAGGTLTVDVTGRGGVPESGVAAVVLNVTVTEPTASGYLTVW
                     PAGQNRPTASNLNFLPAETAPNLVVAQVGADGSVNIFNSAGRTHVVVDVQGWVARPEG
                     AGITAVVPATTNLYSAGHVVSVTGGEAGGTVRLNGAAKVPTVGGHLITPTVGDRPGVT
                     GRVTAVTLRPDGTFDVAWVPAALDQVYDDLVIEIEVALEESPAGDKLDAGATTQAADP
                     MWDCGSGVTLPPIFSQTVGFSNWKVRHEVRLQERYMEFIPSADITVGIDIPVGFSANC
                     ELTPAYRHTHAKRIPIPGTSLVMEV"
     assembly_gap    5094..5111
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          944 a         1636 c         1486 g          881 t
ORIGIN      
        1 cgctgatcgc cgtggtgctc gccgtacccg tcagcctcgg tctggcgctg ttcaccacgc
       61 aggtcgcacc gaagtggctg cgccgcccgc tggtcacctt gaccgacatc gtggccgtgg
      121 tgccctcggt ggtgttcggt ctgtggggcg tcatctattt ggcgccgagg ctggtcgggg
      181 tgttcaagac gctcaacgga tggttcgacg gcgtacccgt gctgtcgagc atcttcggcc
      241 gcgtcggcgg cggccgttcg ttcctgacca ctgggttgat cctggcggtg atgatcacgc
      301 cgatcatcac gtcgatcgcc cgcgaggtga tcgacaccac gccgcaagcc gaccgtgacg
      361 gcgccctcgc cctgggagcg acgcggtggg agatgatcac cggtgcggtg ctgccccact
      421 cggcgggcgg catcgttggc ggcgtcatgc tcggcctcgg ccgggcgatg ggtgagacga
      481 tcgccgccgc gctggtcatc ggttcgtcac cggcgatcac cgccaacatc ttcagttccg
      541 gcgactcgct ccccgcggtg atcgccttcc agtggggtga ggcgaccagt gacgagaagc
      601 gcagcgccct gatcgcgatg ggcctgacct tgttcgtgtt gaccctgctg gtcaacttcg
      661 ccgccaccgc gatcgtcaac cgggcgatga agcggatgca gggagcgtcg tgatgaccgc
      721 tgcatccccc accgcgacca tcccgagccc gttgacccgc ctcagcgtga cggacctggg
      781 tggtgtccgt ggccgtcgcc gggccaccaa tgccgtcata accgtgctga tgggcacatc
      841 ggtcgtggtg atcgccctgg tgttgttgct ggtgctgtgg accgtggtgt ccaaaggttg
      901 gtcgatcgtg gccgggcagt tcccggcgtg gttnnnnnnn nnnnnnnnnn catccaactc
      961 tccgcccgtc gccccggtcc cggcatgaag gccgcgatcg tcggcacgat cgtcaccacg
     1021 gtcggcgcgt cgctgatcgc catacccctc ggcatcctcg gcgcggtcta cctcaacgag
     1081 tacggcaagc gttcggtgtt cgcccgcgtg ctgcggttct tcaccagcgt catggccggt
     1141 gtgccctcga tcgtgatggg tctgttcatc tacgtgttct gggtggtgcc gcgcggaacg
     1201 gccgggctga acagcttcag cggcgcgctc gcgctcgcgt gcctgatgtt gccgatcgtg
     1261 gttcgctcga ccgaagagat gctgcgcctg gtgccgcaga acctgcgcga cgccagcctg
     1321 gcattggggg ccaccggtag ccgcacgatc ctcgggatcg tgatccccaa ggccgcgccg
     1381 gggatcatca gcggttgcct gttggctgtt gcccgtgctg ccggtgagac ggcaccgctg
     1441 atcttcaccg tcggcgcggc gaagtcgacc aactggaact tgttcgacgg caccaccacc
     1501 acgctcagcc tgcagatcta cgccaatgcc aaggagagct tcgccaccgc ccaagaacga
     1561 gcctggggct cagccctcac cctgatcgcc ctcgccgtcc tctttaccgt ggtcgcccgc
     1621 ctgatgtccg cccgcctcac caagagctca taaacctcag ggcgtgaaag acggcgacaa
     1681 gtctcagcac ccgtcattcc acaacggatc cttcgcggtc aaccaagcga cgagctcccc
     1741 gatctccgca gtcaaacagc cattcccgcg gagccaaagc tcctgcaagt tattgagatg
     1801 cgctatattt gtcgaaatcg gaccagtcag ttgattggtt tgtagcaata gactctgtag
     1861 tccctgtata gacattacct gagagggaat cgagcctgcc agttgattag agtggagcca
     1921 aagggcttga agattggcaa gcttgcccag ttgggcaggg attgaccccg tcagctgatt
     1981 gtggcccaaa tccaagaact ggagggcgga gagattcccc agctgagttg gaatcgaccc
     2041 agtcagacga ttgctaccca aactaagagc cttgatgttc actaaactgc ccaactcgac
     2101 cggaacagaa cccgacagct gattgttagt gagatagagg ttctgtaggt gagtaagatt
     2161 caccaactgc aacggaatcg ccccagtaag tcgattgcca gatagatcca gatctctgag
     2221 attgaccaga ctgcctagtt cagcagggat cgatcccgaa aggtgattat tgccaagttg
     2281 gaggccgcga agatcgtcaa ggttcccgag ctgagccgga attgacccac ccaactgatt
     2341 atcactgaga atcagatact caaggtccac caggtcctct aatgtgttcg gaatcgaccc
     2401 ggtcaactga ttgccttgga gtaatagggt tcgaatcgaa ccaccctcac aggtcacacc
     2461 atgccatgta cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng
     2641 ccggggagtt ttgctcttgt ttcagcaaac tcgtaggaat aggacttcca ccagagttca
     2701 aggcgaacgc ctgccgacag gacaaagtcg agtcccaggc gatgacacac gtcaatgtcc
     2761 gtcacctcga cccagagacc gagctcgata ccggcctcga cgtacacacc gaccaagtag
     2821 ccgaacaaca ccgagatgtc gacacccatg annnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngac ctccatcaca
     3061 aggcttgttc ccggaatcgg aatacgcttg gcatgcgtgt gacgatatgc cggggtcaac
     3121 tcgcagttgg cagaaaaccc aactggaatg tcgataccga ccgttatgtc agctgacgga
     3181 atgaactcca tgtatcgctc ttgcaatcgg acttcgtggc gaaccttcca gttagagaaa
     3241 cctacggttt ggctaaagat cggaggcaac gtcacgccac taccgcaatc ccacattggg
     3301 tcagccgctt gcgtcgtcgc acccgcgtcg agcttgtcac ccgccgggga ctcctccaac
     3361 gccacttcga tctcgatgac gagatcgtcg tagacctgat cgagcgcggc gggcacccac
     3421 gcaacgtcaa aagttccatc agggcggagc gtcaccgccg tcactcgccc ggtgacgccc
     3481 ggacgatcac caacggtcgg ggtgatcaga tgcccgccca cagtcggaac cttggcggct
     3541 ccgttcaggc gaaccgtgcc cccggcctca ccacctgtca ccgagaccac atgccctgcc
     3601 gagtacaggt tcgtggtcgc tggcactaca gcagtgatgc cagcaccctc gggacgcgcc
     3661 acccatcctt gaacatcgac gaccacatgg gttcgtccag cagagttgaa gatgttgacg
     3721 ctcccatcgg caccgacctg ggcgaccacc aggttcggtg ccgtctcagc tgggaggaag
     3781 ttcagattgg aagccgtcgg gcgattctga ccggcaggcc agaccgtgag atacccactc
     3841 gcggtcggct ctgtgaccgt cacgttgagc acgacggccg cgacccccga ctccgggacg
     3901 cctccgcggc cggtcacgtc aaccgtcaac gtgccaccgg ccgcgagcgc tccggaacga
     3961 gcagccaacc cgtcgatggt ggcgccgtcg gcgcgagtat ccgccaaccg tgccggagtc
     4021 agcgcgaagt aaccgctgtt gagcgtgaac catccagcaa cgtcgacaac cacatcgacc
     4081 gcgcctgaag cgttgaacac gctgatctgc ccgccttcac cgacacgagc aaccaccaga
     4141 ttcggccgca ccgccccagg gctgaggttc aggttggacg cccacggacg cggagcaccc
     4201 gtcggccaca cggtcaggta cgacgcggtg gtgggcgtca ccgccgtgac gttgacaacg
     4261 acagcggtgg catccgccgg aactcgggcg cggccggtga cctgtacgtc gagcaccgtg
     4321 cccgaaccga gcgcgccgct gcgcgcacca cgaccatcta ccgtctcccc gtccgcgcgg
     4381 gtatcagcaa ggcgcgaggg ctgcatcggg gcgaaccccg agatcgccgg gaaccagccc
     4441 gcaacatctg caatcacgtg cgcggaccct gccgcgttga agatcgacac cttcccgtcg
     4501 agaccgagtt gtgcagtgac caggttcggc acggtctggt cagcccgata gttgaggttg
     4561 gatgcgttcg gccgcggcgc acccgtgggc cacacggtga ggaacgaccc gacggtcggt
     4621 tgatcgacgg tgacgttgag caccaccgcg ccggccccgg tcgtcgggat acccgcgacg
     4681 cctgccaacg tcagctcgat cacgccgcca gcaccgagcg ggccgacagc acccagaccc
     4741 tcgcgagtat cgagcaccct caccggtgag aacgactcca acacagcccc gctgtatgac
     4801 aggttcggag cgccaaccgc ggcgggccgg gctgcaaccg ggacaggagc agcggcagca
     4861 tccaccgccg cgtcggccgg ctcggccgtc ggtgtcccga tcgccagcgc acccagcgcg
     4921 acaccgaaca cagcgaggat gcacatcacc cgtcgcaaca gaggcacagc ggcgcttggc
     4981 gcggccgatc gagcccgtct tttcaacctg gaacctggcg gcaccggcca actcccgtga
     5041 ccccgaccag tgttcacctc agacctcccg ccaccggacc cgccgcgaac catnnnnnnn
     5101 nnnnnnnnnn nggcggcgat aaccacccct cacagccacc tgccatagtt tcgccaaggc
     5161 tgctctctgt ctgagacgat gtaggtgtta cggatcgaca actgcgatga ggtcgccgta
     5221 cttggcgatc cagagcacga cccggagcca gatcatcgtg attggttggc ggtttggctg
     5281 gattgagatt gtcgtcaacc atagccctca ctcggcgcgt ggatcgtcgg gctgtcatct
     5341 gcctgg
//
LOCUS       DAORNF010000134         5338 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_11424_length_5338_cov_1.825478, whole genome shotgun sequence.
ACCESSION   DAORNF010000134 DAORNF010000000
VERSION     DAORNF010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 5338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5338
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11424_length_5338_cov_1.825478"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>238)
                     /locus_tag="PLV68_06755"
     CDS             complement(<1..>238)
                     /locus_tag="PLV68_06755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRE00982.1"
                     /translation="QFWISVASGRTLAALAGLRISEIANLNIADAMLHDKEPRLFVRA
                     GKGGKDRIIPLHPLIAEYMPARRRGPITSVSTDEV"
     assembly_gap    239..292
                     /estimated_length=54
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<293..565)
                     /locus_tag="PLV68_06760"
     CDS             complement(<293..565)
                     /locus_tag="PLV68_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase N-terminal SAM-like
                     domain-containing protein"
                     /protein_id="HRE00983.1"
                     /translation="MNGQQLITKFLQYQAGRGFSPKTAKRRQSTLARFLEHIDPLPLD
                     QVTLQMTDEWLLQFTSPATRHAYQSDLAAFYRWAVRRSVVVHNPMVD"
     gene            716..>1216
                     /locus_tag="PLV68_06765"
     CDS             716..>1216
                     /locus_tag="PLV68_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012231547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HRE00984.1"
                     /translation="MPEAVIVATSRSPIGRAGKGSLVSLRPDDMSAQIVRALMAKVPQ
                     VGAGDIEDLIMGIGQPAGEGGFNIGRMVALLAGLDDVPGVTVNRYCSSSLQTIRMAAH
                     AIKAGEGDCFIAAGVETVSRYAAGASDTAPNPIFKEPGARTRLRAEGGQPDWTPPTGL
                     PDAYIAM"
     assembly_gap    1217..1233
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1234..1918
                     /locus_tag="PLV68_06770"
     CDS             <1234..1918
                     /locus_tag="PLV68_06770"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012231547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HRE00985.1"
                     /translation="VRELENVSREDMDAFAKRSQDLASANIANGWFAEEITPLTLPDG
                     TVVSTDDCPRAGTTLEALAGLKPAFRPDGQVTAGNACPLNDGAAAVMVMSDTKAKQLG
                     ITPLARVVASGVSGLNPEIMGLGPVAAVRQALGRAGMTIDQIDLVEINEAFAAQVLPS
                     ARQLGIPLDKLNVHGGAIALGHPFGMTGARIMTTLIHGLQSTGTTYGLETMCVGGGQG
                     MAMIVERLS"
     gene            2309..3088
                     /locus_tag="PLV68_06775"
     CDS             2309..3088
                     /locus_tag="PLV68_06775"
                     /inference="COORDINATES: protein motif:HMM:NF013625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HRE00986.1"
                     /translation="MPGRCHDPAGRREPQRLQETLATLGLFDGTANGVARDDTEEAIR
                     RYQAATRSLTADGLAGPNTLAALGIWSGSTSVAWINGAAIFTNNGPTEVVVSSSSGGT
                     GVVGTAPPGPWPSPMHDDQQWRLTAEGIPAYGNRTPCSREQADMVAYQFAIDGADVDT
                     QQWAVYVASREGGCRYDAVNQNAATRDDSHCTFQLNALSGMFAPYGELGRLGWTAESV
                     KYSLDNCANAASDLWVYCGRGPWTPPYSCRPPWREPTTAES"
     gene            complement(3186..>4013)
                     /locus_tag="PLV68_06780"
     CDS             complement(3186..>4013)
                     /locus_tag="PLV68_06780"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRE00987.1"
                     /translation="DMDALTQIDAWPVGFATAAVVSANGVIASRGDTGRVVRLASISK
                     PLAAWACMIAVEEGTASLDDAVGQPGCTLRHLLAHAGGYGFDGPNPITRPERRRIYSN
                     TGIELAAGAIQAASGIGFADYLREAVFEPLGMDTALLRGSPAHGVRSSAGDLTRFATE
                     LLAPRLVTADTAAMATAVQFPALSGMVPGIGSFDPCPWSLGFEVHGAKYPHWMGTSNS
                     PATYGHFGGSGTMFWVDPEIGVALIALTDRAFDEWAAEATAAWRSLSNAVIANITSR"
     assembly_gap    4014..4181
                     /estimated_length=168
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4182..>5338)
                     /locus_tag="PLV68_06785"
     CDS             complement(<4182..>5338)
                     /locus_tag="PLV68_06785"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase class II"
                     /protein_id="HRE00988.1"
                     /translation="DPAALDQAHKQLSQFPPLVFAGEARSLQAGLANVAAGNAFLLQA
                     GDCAESFEEFSANNIREKLRVILQMAVVLTYSLGVPVVKVGRMAGQFAKPRSSPFERF
                     GDLELPSFRGHIVNGEAPLAAARVPDPERLVQAYHQSASTLNLVRAFTKGGYADLNRV
                     HAWTQEFVATSPEGQKYEQLAAEIDRALKFMRACGIDTESNSRLHEVDVFTSHEALLL
                     GYEEALTRQDTTTGRWYDCSAHMLWIGERTRDLDGAHIEFLRGVGNPVGCKIGPTTSA
                     EYVLDLCQALNPAQIPGRITLISRMGAGKVEDSLRPLLRAVRDAGHPVVWACDPMHGN
                     TYTSQSGRKTRSFDAICDEIAGFVRAHTAEGTWPGGIHVELTGDDVTECVG"
BASE COUNT          796 a         1697 c         1807 g          799 t
ORIGIN      
        1 acctcatcgg tgctgaccga ggtgatgggg ccgcggcgcc gcgctggcat gtactcggcg
       61 atgagtgggt ggagcgggat gatccggtcc ttgccgccct tcccggcgcg cacgaacagc
      121 cgaggctctt tgtcgtggag catggcgtcg gcgatgttga ggttggcgat ctcggagatc
      181 cggaggcctg cgagcgctgc aagggtgcgt cctgaggcga cggaaatcca aaactgttnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngtccacca
      301 tggggttgtg gaccacgaca ctccgcctga cggcccagcg gtagaacgcg gcgagatcgg
      361 attggtatgc gtggcgggtt gccggactgg tgaactgcag cagccactcg tcggtcatct
      421 ggagggttac ctggtcgagc gggagcggat cgatgtgctc aaggaagcga gccagggttg
      481 actggcgtcg cttcgccgtc tttgggctga atccgcgtcc ggcctggtat tggaggaact
      541 tcgtgatgag ctgctgtccg ttcatgatgt tactcatcga acaccccgcc agattcttga
      601 gcaggcgttc atatttcggt caggctgcga gcaggagcgg gcgcggatac cggtaggtag
      661 attgggccga aaccggtcgg cgaggatgcc gacgcaatcc acagggagaa tcccaatgcc
      721 tgaagctgtc atcgtcgcca cctcccgcag cccgatcggc cgtgctggca agggctcgct
      781 cgtgtcgcta cgccccgacg acatgtcggc ccagatcgtg cgtgcgctga tggccaaggt
      841 gccccaggtc ggcgcgggcg atatcgaaga cctgatcatg ggcatcggtc aacccgctgg
      901 cgaaggcggc ttcaacatcg ggcggatggt cgcgctgctg gctggcctcg acgacgtgcc
      961 gggtgtgacg gtcaatcgct actgctcgtc cagcttgcag accatccgta tggccgctca
     1021 cgcgatcaag gctggcgagg gtgattgctt catcgcggcg ggcgtcgaga cggtcagccg
     1081 gtacgccgcc ggtgcgagcg atacggcccc caacccgatc ttcaaggagc ccggcgcgcg
     1141 cacccgcctg cgcgccgaag gtggtcagcc cgactggact ccgcccaccg ggctgcccga
     1201 cgcctacatc gccatgnnnn nnnnnnnnnn nnncgtgcgc gagctcgaga acgtgagccg
     1261 tgaggacatg gacgcgttcg ccaagcggtc gcaagacctg gccagcgcca acatcgccaa
     1321 tggctggttc gccgaggaga tcaccccgct caccctgcct gacggcacgg tcgtgtccac
     1381 cgacgactgc ccgcgtgcgg gcaccacgct ggaagccctc gccggtctga agccggcctt
     1441 ccgcccggac ggccaggtca cggcgggtaa tgcctgcccg ctcaacgacg gggcggccgc
     1501 ggtgatggtg atgagcgaca ccaaggccaa gcagctcggc atcacgccgc tggcccgcgt
     1561 cgtcgcctcc ggtgtgtcgg ggctcaaccc cgagatcatg ggcctcggcc cggttgccgc
     1621 ggtgcgccag gcactgggcc gggcggggat gaccatcgac cagatcgacc tggtcgagat
     1681 caacgaagcc ttcgcagccc aggtgctgcc gtcggcgcgt cagctcggca tcccgctcga
     1741 caagctcaac gtgcacggcg gcgcgatcgc gctcggccac ccgttcggca tgaccggggc
     1801 gcggatcatg accacgctca tccacggtct gcagtcgacc ggcaccacct acgggttgga
     1861 gacgatgtgc gtcggcggcg gccagggcat ggccatgatc gtcgaacggc tctcctgagc
     1921 gccagaatgg gccgatgcgc ttcagggcac ggtcagcagc gatcgcggcg gcagtagccc
     1981 tcggcggtgt cgtcgcatcc gccggggccg cttcggtggc cccggtgcag gcggtctctc
     2041 agccgaccgc gaccggatgc gccgtcaatg ctgagctgac accgggctcg accggcgccg
     2101 gcgtcatctg cctgcagtac gcgctgggca tgatggggct gtccgatcgg ccgatcaccg
     2161 gcgagtacga cgagaccacg gctgatctgg tccgctggtt ccaagccaac aacgcaccgc
     2221 tgcgggtcga cggcaacgct ggtgtcgcga ccctcgaagc gatcggcatc ggggcgtcgt
     2281 cggcaccgca agcgatcccg atcgccacgt gccgggccga tgccacgatc cggccgggcg
     2341 acgagagccc cagcgtctgc aagagaccct ggccaccctc ggcctgttcg acggcaccgc
     2401 caacggcgtg gcccgcgacg acaccgagga agcgatccgc cgttaccagg ccgcgacccg
     2461 gtcgctgacc gccgacggct tggccggtcc caacacgctc gccgcgctgg gcatctggtc
     2521 gggcagcacg agcgtggcgt ggatcaacgg cgccgcgatc ttcaccaaca acggcccgac
     2581 cgaggtcgtg gtcagttcca gcagcggcgg caccggtgtg gtcggtaccg ctccgccggg
     2641 accgtggcca tcgccgatgc acgacgacca gcagtggcgc ctcacggcgg agggcatccc
     2701 cgcctacggc aaccgcaccc cgtgctcgcg agagcaggcc gacatggtcg cctaccagtt
     2761 cgccatcgac ggtgccgacg ttgacacaca gcagtgggcg gtgtacgtgg catcgcgcga
     2821 aggcggctgc cgctacgacg cggtcaacca gaacgcggcc acgcgcgacg actcgcactg
     2881 cacgttccag ctcaacgcgt tgtcgggcat gttcgccccc tacggcgaac tcggccggct
     2941 cggttggacc gcggagtcgg tcaagtactc gctcgacaac tgcgccaacg cggccagcga
     3001 cctgtgggtc tactgcggcc gcggcccgtg gaccccgccc tacagctgcc gccccccctg
     3061 gcgagaaccc accacggctg agagctgaac gccacagagc gtgttcagtc ggccgccgct
     3121 gccggggtga cggagcgcgt ggagagatcg agtcgatcga cccccagacc tcatggagcg
     3181 tggggtcagc gcgaggtgat gttggcgatg acggcgttgc tgagggagcg ccaggctgcg
     3241 gtggcttcgg cggcccactc gtcgaaggcg cggtcggtga gggcgatcag tgccacgccg
     3301 atctcgggat cgacccagaa catcgtgccc gaaccgccga agtggccgta ggtggcgggg
     3361 gagttggacg tgcccatcca gtgcgggtac ttggcgccgt ggacctcgaa gccgaggctc
     3421 cacggacagg ggtcgaacga gccgatcccg ggcaccatgc cgctcagcgc cgggaactgc
     3481 accgcggtcg ccatcgccgc ggtgtcggcg gtgacgagcc gcggcgcgag cagttcggtc
     3541 gcgaagcggg tcaggtcacc ggcgctggag cgcacgccgt gggccggtga accgcgcagc
     3601 agggcggtgt ccatccccag cggttcgaac accgcctcgc ggaggtagtc ggcaaagccg
     3661 atgccgctgg ccgcttggat cgcgcccgca gcgagctcga tgccggtgtt ggagtagatc
     3721 cgtcgccgtt cgggccgggt gatcgggttc ggtccgtcga agccatagcc gccggcgtgg
     3781 gccaacaagt ggcgcaacgt gcaacccggt tggccgaccg cgtcgtcgag gctggccgtg
     3841 ccctcttcga ctgcgatcat gcacgcccac gcggcgagcg gcttggagat cgacgccagc
     3901 cgcacgaccc gccccgtgtc accacggctg gcgatcaccc cgttggccga cacgaccgct
     3961 gctgtggcaa agccgacggg ccacgcgtcg atctgcgtga gcgcgtccat gtcnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncgccgacgc actcggtgac
     4201 gtcgtcaccg gtgagctcga cgtggatgcc tccgggccag gtgccttcgg ccgtgtgggc
     4261 ccgcacgaag ccggcgatct cgtcgcagat cgcatcgaag ctgcgcgtct tgcgaccgct
     4321 ctgcgacgtg taggtgttgc cgtgcattgg atcgcaggcc cacaccaccg ggtgcccggc
     4381 gtcgcgcacc gcgcgcagca gcgggcgcag gctgtcttcg accttgccgg cgcccatgcg
     4441 gctgatcagc gtgatccgcc ccgggatctg ggccgggttg agcgcctgac agaggtcgag
     4501 cacgtactcg gccgaggtgg tcgggccgat cttgcagccg accgggttgc ccacgccacg
     4561 gaggaactcg atgtgcgcgc cgtcgaggtc gcgggtgcgc tcgccgatcc acagcatgtg
     4621 ggccgagcag tcgtaccatc taccggtggt cgtgtcctga cgggtgagcg cctcttcgta
     4681 gccgagcagc agcgcttcgt ggctggtgaa gacgtcgact tcgtggaggc ggctgttcga
     4741 ctcggtgtcg atgccgcagg cacgcatgaa cttgagcgcc cgatcgatct cggctgccag
     4801 ttgctcgtac ttctggccct cggggctggt cgcgacgaac tcttgggtcc aggcgtggac
     4861 tcggttgaga tcggcgtagc cacccttggt gaaggcccgc accaggttga gcgtggaggc
     4921 gctctggtgg tacgcctgca ccaggcgttc ggggtcgggg acgcgggccg ctgccaaggg
     4981 cgcctcgccg ttgacgatgt ggccccggaa gctgggcagc tcgagatcgc cgaagcgctc
     5041 gaacggggac gagcgcggct tggcgaactg acccgccatg cgcccgacct tgaccaccgg
     5101 cacgccgagg gagtaggtga gtacgaccgc catctggagg atgacgcgca gcttttcgcg
     5161 gatgttgttg gccgagaact cttcgaagct ctcggcgcag tctccggctt gtagcaagaa
     5221 cgcattgccg gcggccacgt tggccaaccc ggcctgcagg ctgcgggcct cgccggcgaa
     5281 caccagcggg gggaattggc tgagctgctt gtgggcctgg tcgagcgcgg ccgggtct
//
LOCUS       DAORNF010000135         5337 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_11428_length_5337_cov_1.528966, whole genome shotgun sequence.
ACCESSION   DAORNF010000135 DAORNF010000000
VERSION     DAORNF010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 5337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5337
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11428_length_5337_cov_1.528966"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="PLV68_06790"
     CDS             complement(<1..212)
                     /locus_tag="PLV68_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HRE00989.1"
                     /translation="MIAASLNATLGRAALLFGLVCAAFGALSTVYGIRVGDRRLLRSS
                     LRYSIGVAVAAIAAFAIMERALIQRD"
     gene            complement(213..>420)
                     /locus_tag="PLV68_06795"
     CDS             complement(213..>420)
                     /locus_tag="PLV68_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00990.1"
                     /translation="GGAFQECIAVVVRGRMLGDAADPSTWVFHGDELEVKHGNDYQER
                     TSGDRPTEAEERAEACRLAAAGSL"
     assembly_gap    421..507
                     /estimated_length=87
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<508..760)
                     /locus_tag="PLV68_06800"
     CDS             complement(<508..760)
                     /locus_tag="PLV68_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00991.1"
                     /translation="MDLTPRASDPSAPAAPLAARVAKRRKWLSLALLAAVVVAGGVVV
                     TMFLTNALDYYCNVDEVGPAGAAGVKSNCELDRQIRIQGR"
     assembly_gap    825..963
                     /estimated_length=139
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<964..1675)
                     /gene="ccsA"
                     /locus_tag="PLV68_06805"
     CDS             complement(<964..1675)
                     /gene="ccsA"
                     /locus_tag="PLV68_06805"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HRE00992.1"
                     /translation="MATLHDSLGRDSTGRDSSDPGSTGPASTGTRFTRVLGIATLVSM
                     VALTLFGLVWSPADVTQGESVRILYIHVGTVWVAYMAFVVTAIASLLYLWKRTSSLTW
                     DRIAGASAEVGVLFMALTLFAGALWGRLSWGKFWVWDARVTTTAFLFITYIGYLAVRG
                     LGGSHQQRARRSAVIALLAVLEIPLVHFSVKLWRGLHQEGSVAGTTDVKLENLMLFSL
                     FLALIAFTLLYVWLVIHRQ"
     gene            complement(1679..2356)
                     /locus_tag="PLV68_06810"
     CDS             complement(1679..2356)
                     /locus_tag="PLV68_06810"
                     /inference="COORDINATES: protein motif:HMM:NF015344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HRE00993.1"
                     /translation="MLRVARLIAAKDLRIEVRSRVVTNQVLPFAAIVMVMFAFALNNF
                     DTLEVVAPGLIWLATLFSLLILVQRAFAIETADGATDALRAAGVDPAGIFLGKSMALA
                     VQLAVLEVLLLAIAFVLYQSSVGAGGVVLLVTTFVVATLGLALVGTLYGGLAAGIRGR
                     ETLLPLLLLPVVAPVLIGATRATEAAFGTRGAVVADGWPWLGLLAVFAGVFGVGGTLA
                     FGPLIEE"
     gene            complement(2356..>2815)
                     /locus_tag="PLV68_06815"
     CDS             complement(2356..>2815)
                     /locus_tag="PLV68_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRE00994.1"
                     /translation="VLGHDLSTDRESVRRRVGLLSHANGLYADLTVAENVRFWAATVG
                     ASPGEIEAAMQRMGVATRLADVAVSKLSAGQKRRTALACLVARRAELWLLDEPHSGLD
                     AAGRDDLDATLRAATAAGATIIIASHELERAGALATRTVEVVAGRIQEVA"
     assembly_gap    2816..2881
                     /estimated_length=66
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3230..3304
                     /estimated_length=75
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3305..4742
                     /locus_tag="PLV68_06820"
     CDS             <3305..4742
                     /locus_tag="PLV68_06820"
                     /inference="COORDINATES: protein motif:HMM:NF033747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class E sortase"
                     /protein_id="HRE00995.1"
                     /translation="VPAAAVVPTAPAAPASAATELVDTSAPDAAASTPAPIWVPPGRV
                     KRNGKPKRRRRMNRWDRPPEPHDWRWAVGHIGRLLITVGLLMFGFVAYQLWGTGIETA
                     QAQSRLDDDFDTYLVANGITPDTIAPAPPGEVVIPTIPTRPDPSQPSQPTTPDGPTIT
                     AAPAQVQDYGDVDPGDALMRLTIPAIDLDWKVVAGVANKDLAKGPGHFPDSPLPGQYG
                     NAAIAGHRTGHGGPFFSLNELKRGDEIQIRTRIGGAYVYIVTGLEVVTPADYHVITDS
                     NPDVATLTLVTCEPRYTSRNRLIVHAILDETRGSPVGEPLLFYGDPNPAPVTPVLPDD
                     PGITDETDLSAVSAVSTPASALDPFSTDDIVTETTLAAVVTTAATTATTEAATTTTIA
                     DGSRPASQRDEFGTEAAFSQGWFDDGAAWPHVIGWGLALALIAIGAYQLAKFYRRLYL
                     AFLVGAIPFVIVLYFFYENANRLLPAAI"
     gene            4792..5046
                     /locus_tag="PLV68_06825"
     CDS             4792..5046
                     /locus_tag="PLV68_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HRE00996.1"
                     /translation="MTMLSFRVDDREAAEAQHWAEALGVDRSELLRDALRRHLDRLTA
                     EGDADRWNAQPLDPGEMALAAIATWGPAEDWSDWGDDATR"
     gene            5033..>5337
                     /locus_tag="PLV68_06830"
     CDS             5033..>5337
                     /locus_tag="PLV68_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HRE00997.1"
                     /translation="MQRGEIWFASAPDGDRPVLVLTRDPVADRIGAVVVAALTRTERG
                     LVSELRLTPADGVPTDSVVNFDNIHTLPREAFRRRIAKLPTRRLGEICRTLQAATGC"
BASE COUNT          862 a         1787 c         1626 g          695 t
ORIGIN      
        1 aagtcgcgct ggatcagcgc ccgctccatg atcgcgaagg ccgcgatcgc cgccaccgcg
       61 acaccgatcg agtaacgcag cgaactgcgc agcaacctgc ggtcgccgac ccggatgccg
      121 tacacggtcg acagcgcccc gaaggccgcg cacaccagac cgaacagcag cgccgcccgg
      181 cccaacgtgg cgttgagtga cgctgcgatc acctacaggc tccctgccgc ggccagccgg
      241 cacgcctcgg cacgttcttc ggcttcggtc ggccgatcgc cactggtgcg ctcctggtag
      301 tcgttgccgt gcttgacctc gagctcgtcg ccgtggaaga cccacgtcga cgggtcggct
      361 gcatcgccca gcatccggcc ccgcaccacc acggcgatgc actcttggaa cgccccgccg
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      481 nnnnnnnnnn nnnnnnnnnn nnnnnnncgc gaccttggat gcggatctgg cggtcgagct
      541 cgcagttgct cttcacacca gcggcacccg cagggccgac ctcgtcgacg ttgcagtagt
      601 agtcgagcgc gttggtgaga aacatcgtga ccacgacgcc gcccgccacg accactgccg
      661 ccaacagtgc gagcgacagc cacttgcgcc gcttggcgac ccgcgccgcc aacggtgccg
      721 cgggcgcgga ggggtcgctg gcgcgcggtg tcaggtccac ggcttgtcct cgggcggaag
      781 ctgacgggcc ggggggcggg gcggggtgcc atttgcgggg aggtnnnnnn nnnnnnnnnn
      841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      961 nnngctggcg atggatcacc agccagacgt agagcagggt gaacgcgatc aaggcgagga
     1021 acagcgagaa cagcatcagg ttctccagct tgacgtcggt ggtgccggcg acgctgccct
     1081 cttggtgcag gccgcgccac agcttgacgc tgaagtggac cagcgggatc tcgagcacgg
     1141 cgagcagggc gatgaccgcc gagcgccggg cccgctgctg gtggctgcca cccagcccgc
     1201 gcaccgccag gtagccgatg taggtgatga acaagaacgc ggtggtggtg acccgggcgt
     1261 cccacaccca gaacttgccc cacgacaggc gcccccacag cgccccggcg aacagcgtca
     1321 gcgccatgaa cagcacgccg acctcggcgc tggcacccgc gatgcggtcc cacgtcaacg
     1381 acgaggtgcg cttccacaga tagagcagcg acgcgatggc ggtgacgacg aaggccatgt
     1441 aggcgaccca caccgtgccg acgtggatgt acaagatgcg cacgctctcg ccctgggtga
     1501 cgtcggccgg cgaccacacc aagccgaaca gcgtcagggc gaccatgctc accagcgtgg
     1561 cgatgccgag cacccgcgtg aaccgggtgc ccgtcgaggc gggaccggtc gagccggggt
     1621 cgctggagtc gcggcccgtg gagtcgcggc cgagtgagtc atgaagagtg gccatgtgtc
     1681 attcctcgat caggggtccg aatgccagcg tgccgccgac gccgaagacg ccggcaaaga
     1741 ctgcgagtag gcccaaccag ggccaaccgt ctgcaaccac cgcaccgcgg gtgccgaacg
     1801 cagcttccgt tgcgcgggtg gcaccaatca gcacgggcgc taccaccggc agcaacagca
     1861 agggaagcaa cgtctcccgt ccgcggatgc cggcagcaag gccgccgtac agcgtaccga
     1921 cgagggccag cccaagggtc gcgacgacga aggtggtgac caacagcacg acgccgcccg
     1981 cgccgacgct ggactggtac agcacgaagg cgatcgcgag cagcagcacc tcgagcactg
     2041 ccagctgcac ggccagtgcc atcgacttgc ccaggaagat gccggccggg tcgaccccgg
     2101 cggcgcgcaa cgcgtcggtg gcaccgtcgg cggtctcgat cgcgaacgcc cgctgcacca
     2161 ggatcagcag gctgaacaac gtcgccagcc agatcaggcc gggggccacc acttcgaggg
     2221 tgtcgaagtt gttcagcgcg aacgcgaaca tcaccatcac gatcgcggcg aacggcagca
     2281 cctggttggt gaccacgcgg ctgcgtacct cgatgcgcag gtccttggcc gcgatcagcc
     2341 gcgccacccg cagcatcacg ccacctcctg gatgcgaccg gcgacgacct cgacggtacg
     2401 ggtcgccagg gcgcccgccc gttccagctc gtggctggcg atgatgatcg tcgcccccgc
     2461 cgcggtggcg gcgcgcaacg tggcgtcgag gtcatcgcga ccggcggcgt cgaggccgga
     2521 gtgcggttcg tcgagcagcc acagctcggc ccggcgcgcg accaggcagg ccaaggcggt
     2581 gcgccgcttc tgtcccgccg agagcttgct gaccgcaaca tcggcgagcc gggtggccac
     2641 gcccatgcgc tgcatcgcgg cctcgatctc gccgggcgat gcaccgactg tcgcggccca
     2701 gaaccgtacg ttctcggcca cggtgagatc ggcgtagaga ccgttggcgt gcgacagcag
     2761 accgacccgg cggcggaccg actcacggtc ggtactcaga tcatgaccga gcaccnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 ngttcggccc ttggaggagc acgatctcac ccgggtgcac ggtgaggtcg gcgccagcca
     2941 acaccgggaa ctggcccagt acggccaccg cattcgaaag ctggacgacg gtatccatgg
     3001 gcctgcggtg aggctagttg tcggcgccac catccaccca gccgccgggc cgcgggctga
     3061 tcaggccagc ctgcacagca gatcgtgatg tcgagccagt agggtcgccg accatggcgg
     3121 tggacgacac ccctttcggc gacacacatg acgagctcac ggtgaccccg gtcgagggca
     3181 tccccgtgat cagggtcgat ctcgcgccct acgacgatca ccccggcgcn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnncgtacc cgccgcagcc gtcgtaccca ctgcacccgc cgcacccgcc tcagctgcca
     3361 cggagttggt cgacacgtca gcgcccgatg cggcggcatc gacgccggca ccgatctggg
     3421 tgcccccagg gcgggtgaag cgcaacggca agccgaagcg tcggcgacgg atgaaccgct
     3481 gggatcggcc accggagccg cacgattggc gctgggcggt cggccacatc ggtcgcctgc
     3541 tgatcaccgt cgggctgttg atgttcggct tcgtcgctta ccaactgtgg ggcaccggca
     3601 tcgagaccgc tcaggcccag agccgtctgg acgacgactt cgacacgtac ctcgtcgcca
     3661 acggcatcac gcccgacacg atcgcccccg ccccgccggg cgaggtcgtc atccccacca
     3721 tcccgacccg accggaccca agccagccaa gccaaccgac cacccccgac gggccgacga
     3781 tcaccgctgc gcccgcccag gtgcaggact acggcgacgt cgacccgggt gacgcactga
     3841 tgcgactgac cattcccgcc atcgaccttg attggaaggt ggtcgccggg gtcgccaaca
     3901 aggatctggc caaaggaccc ggtcacttcc ccgacagccc cttgccgggc cagtacggca
     3961 acgcagccat cgctggccac cgaaccggcc acggcggacc gttcttttcg ctcaacgagt
     4021 tgaagcgcgg tgacgagatc cagatccgca cccgcatcgg cggcgcctac gtgtacatcg
     4081 tcaccgggct ggaggtggtc accccggccg actatcacgt catcaccgac tccaatcccg
     4141 acgtcgccac gctcacgttg gtgacctgcg agccgcgata cacatcgcgc aaccggctga
     4201 tcgtgcacgc catcctcgac gagacccgcg ggtcaccggt cggtgaaccc ctgctgttct
     4261 acggcgaccc caaccccgcc ccggtcacgc ccgtgttgcc cgacgatccc ggcatcaccg
     4321 acgagaccga tctgtccgcc gtgtccgccg tatcgacgcc tgcatcggca ctcgacccgt
     4381 tcagcaccga cgacatcgtg accgagacca ctctcgccgc cgtcgtcacc accgcggcca
     4441 ccacggccac gaccgaggct gcgacgacca cgacgatcgc cgacggttca cgcccagcat
     4501 cgcagcgcga cgagttcggc accgaggccg ccttctcgca gggctggttc gacgacggcg
     4561 cggcgtggcc gcacgtgatc ggctggggcc tcgccctggc cctgatcgcg attggcgcat
     4621 accagttggc caagttctac cgccgcctgt atctggcctt cctggtcggc gcgatcccct
     4681 tcgtgatcgt cctctacttc ttctacgaaa acgccaatcg cctcctcccc gccgccatct
     4741 gacaaggtcg tttgaccaag gggtcgccgt catacgcggt accatcgatg tatgacgatg
     4801 ctcagtttcc gcgtcgatga tcgtgaggct gccgaggccc agcactgggc cgaagcgctc
     4861 ggggtcgacc gttcagagtt gttgcgcgac gcgctccgcc gccatctcga ccgactgacg
     4921 gccgaaggtg atgcagatcg ctggaatgcc caacccctcg acccgggcga gatggcgctc
     4981 gcagcgatcg ccacgtgggg gccggccgaa gactggtccg actggggcga cgatgcaacg
     5041 cggtgagatt tggttcgcct cggcgccgga cggtgaccga ccggtgctcg tcttgactcg
     5101 cgatcccgtc gcggaccgga tcggtgccgt cgtggtcgcc gcgttgacca gaaccgagcg
     5161 gggcctcgtc tccgagctcc gactgacgcc tgcggacggg gtgccgaccg acagtgtggt
     5221 caacttcgac aacattcata cgttgccgcg tgaggcgttt cggcggcgca tcgcgaagct
     5281 gccgacccgg cgactgggcg agatatgccg aactctgcag gccgcgacgg ggtgttg
//
LOCUS       DAORNF010000136         5337 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_11429_length_5337_cov_1.188565, whole genome shotgun sequence.
ACCESSION   DAORNF010000136 DAORNF010000000
VERSION     DAORNF010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 5337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5337
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11429_length_5337_cov_1.188565"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="PLV68_06835"
     CDS             complement(<1..208)
                     /locus_tag="PLV68_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00998.1"
                     /translation="MSTPIIIHHANCPDGFGAAWWLTRLVDHNAIIHAATHDDATLPD
                     CTGRDVWIVDFCYQAPLLRELAATA"
     gene            complement(205..576)
                     /locus_tag="PLV68_06840"
     CDS             complement(205..576)
                     /locus_tag="PLV68_06840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE00999.1"
                     /translation="MTALEQLPSWLPPDYEARRIEVRIEADDPEYVGGVTDAHAVWNF
                     HYAVTAVEVALYGGEEVDFAEPASLGGAHTVWQHTGYGDSLDAAAAACLRMIDEHRRV
                     IEDPANALAPQPGETLIGGGQ"
     assembly_gap    711..765
                     /estimated_length=55
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<766..1153)
                     /locus_tag="PLV68_06845"
     CDS             complement(<766..1153)
                     /locus_tag="PLV68_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01000.1"
                     /translation="MGTRPNYVYVASSWRNPVHADVIDALAVAGIDHYDFKNPSGGTG
                     FSWREVMELERVSHDFVPYRANPTICSVCGSVQPMPCLSLPAALTATAGVPAKGADWV
                     PARRYLDMVSHRRAIEAGVKLHGATVH"
     gene            complement(1288..>1858)
                     /locus_tag="PLV68_06850"
     CDS             complement(1288..>1858)
                     /locus_tag="PLV68_06850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01001.1"
                     /translation="PLIPFSRHKAGRATLRCQKDRGGNYAVGEVVADIQFQPLGAGEL
                     LQIDVGPHVDPASSRDEWAPTILMERISRKIIEAPAATLTRSSLTTLFPHAKRAVVDQ
                     AIATLLTEGYIVEARGGRGRGRPFRSLRPFWANGQGPSSDRPDPTPVDHALFDDVEYI
                     APPPPPEWENVLRGPWADPRWVPPDEEGS"
     assembly_gap    1859..1931
                     /estimated_length=73
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1932..>2335)
                     /locus_tag="PLV68_06855"
     CDS             complement(<1932..>2335)
                     /locus_tag="PLV68_06855"
                     /inference="COORDINATES: protein motif:HMM:NF024872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRE01002.1"
                     /translation="STSGKSWLALQWALEVLQHGAVVYVDYEDSEIAIVNRLLALKAN
                     PRHVADRFFYVQPEGSFGPAEKAAMNAVFDRLNPDLIVIDAFVGALTAEGLDENVAQD
                     VDRWQQNVGRWLIQASGGAAILLLDHVKKDPD"
     assembly_gap    2336..2474
                     /estimated_length=139
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2475..2951)
                     /locus_tag="PLV68_06860"
     CDS             complement(<2475..2951)
                     /locus_tag="PLV68_06860"
                     /inference="COORDINATES: protein motif:HMM:NF012974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase N-terminal domain-containing
                     protein"
                     /protein_id="HRE01003.1"
                     /translation="MPAKDHHPPKSNRSRADARVPPHDLAAEAAALGAALLNADAAAG
                     LPDPARFYKPAHQFIAAAIGKLESGTVDVVTVADQLRRDGLLDEVGDLDGLVDLISTT
                     PSIGQWPAYARIVNNTWQLRDIIAAGAAIADLGYSAPDDVAAALARARGDLDQITVE"
     gene            complement(2957..3199)
                     /locus_tag="PLV68_06865"
     CDS             complement(2957..3199)
                     /locus_tag="PLV68_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01004.1"
                     /translation="MSTDHDHDPDECIHGLGPRSACVLCNGRAAREEAEAHRIVQWFA
                     ARYSGRLACGCYTTVGDLIARRADDTFVCLDHSHGR"
     gene            complement(3196..>3385)
                     /locus_tag="PLV68_06870"
     CDS             complement(3196..>3385)
                     /locus_tag="PLV68_06870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HRE01005.1"
                     /translation="DPFCGTGTTLAVAELHGRHAIGIDLDPRNELLLRARMDECRRAL
                     YGTRPEMPGQASLFGAAR"
     assembly_gap    3386..3579
                     /estimated_length=194
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3580..4258)
                     /locus_tag="PLV68_06875"
     CDS             complement(<3580..4258)
                     /locus_tag="PLV68_06875"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HRE01006.1"
                     /translation="MRESNAAHWRAKQIARHAWPHRKSLALIPQAYAMGLAYGRNPIS
                     GAESPAGQWLVRNIIVWHRPNPTVGALGDKFRPSTSYIVVATRDTQRWFDLSAVRRQT
                     ERRASRDGHSVKSGPEGTMTATYDYGAPTTAGAPPLDCWSDEIDEHLTWTMSSQPSRL
                     AHYAMWPPKLAERLILSMCPAEVCAACGEPRRRIEGAYTLDAFRGSNRPQTRRAVAIA
                     DAAGLSDA"
     assembly_gap    4351..4613
                     /estimated_length=263
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4691..4972)
                     /locus_tag="PLV68_06880"
     CDS             complement(4691..4972)
                     /locus_tag="PLV68_06880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01007.1"
                     /translation="MWLAESGIEFVEVHNAHLKMFATGSASADKTVMTHAAGSRCHHL
                     GVAGPANDDEADAWHLRGFGRVGLGLDAPWAEYQWDATRASSVEWLCSR"
     gene            complement(4996..5211)
                     /locus_tag="PLV68_06885"
     CDS             complement(4996..5211)
                     /locus_tag="PLV68_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01008.1"
                     /translation="MAGLDASTKRIGYCAPDGSMHSVTADRLPAHPSPWDLALRCLQL
                     RSRLARIVSLNPPRPTLVVYEAAFAQR"
BASE COUNT          703 a         1497 c         1677 g          736 t
ORIGIN      
        1 gcgctgtcgc tgcgagttcg cggagaagcg gcgcctggta gcagaagtcg acgatccaca
       61 cgtcccggcc ggtgcagtcg gggagggtcg cgtcgtcgtg ggtggcggcg tggatgatgg
      121 cgttgtggtc gacgaggcgg gtaagccacc aggctgcgcc gaacccgtcg ggacagttcg
      181 cgtggtggat gatgatcggc gtgctcactg gccaccaccg atcaaggtct cgcccggctg
      241 aggagcgagt gcgttcgctg ggtcctcgat gacgcggcgg tgttcgtcga tcattcgcag
      301 acacgccgct gcggcggcgt cgaggctgtc gccgtacccg gtgtgctgcc acaccgtgtg
      361 cgcaccgccg agcgaagccg gttcggcgaa gtcgacctcc tcgccgccgt acagggcgac
      421 ctcgacggcg gtaaccgcgt agtggaagtt ccacactgcg tgcgcgtcgg tgacaccgcc
      481 gacgtactcc gggtcgtcgg cttcgatgcg cacctcgatg cggcgggcct cgtagtcggg
      541 gggaagccag gagggcagct gctccaacgc ggtcatcact cgacccccag ccacgcgagc
      601 acctcagcga tcgacgtcgc gaagtggtca accatccggt acatcagctc cggctccacc
      661 gggtcctcga gcagcacgat cgtccgcttc ccggcaccga ctgcccaccc nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnaatgc accgtcgcgc
      781 catgcagctt cacgcccgct tcgatggcgc ggcggtgcga caccatgtcg aggtaccggc
      841 gggcaggcac ccagtcggca cccttggccg ggacgccggc cgttgctgtc agcgcggccg
      901 gaaggctcag acagggcatc ggctgcacag acccgcagac cgagcagata gtcgggttcg
      961 cgcggtaggg cacgaagtcg tggctgacgc gctcaagctc catgacttcc cgccacgaga
     1021 acccggtgcc accggagggg ttcttgaagt cgtagtggtc gatgccagcg acagcgaggg
     1081 cgtcgatgac atcggcgtgg acggggttgc gccagctgct ggcgacatag acgtagttgg
     1141 gtcgggtgcc catggttctc cttggtttgc gggttgcgat ctggtgtggg ggtttcgccc
     1201 ggccccggtc ggcacaacat gggaccggtc gggtggggac ggcggcgcgc acgggagata
     1261 gttccgtggc acccccacgt gccgccgtta cgacccctcc tcgtcaggcg ggacccagcg
     1321 agggtccgcc catgggccac gcagcacgtt ctcccactcg ggcggtggtg gcggcgcgat
     1381 gtactcgacg tcgtcgaaca acgcgtgatc caccggcgtc gggtcgggac ggtccgagga
     1441 cgggccttgc ccgttcgccc agaacgggcg cagcgatcgg aacggacgac cccgaccccg
     1501 accgcctcgg gcctcgacga tgtagccctc ggtcagcagg gtcgcgatcg cctggtcgac
     1561 cacggcacgt ttcgcgtgcg gaaacagggt ggtgagcgac gaccgagtga gtgtcgccgc
     1621 cggggcttcg atgatcttgc gggagatccg ttccatgagg atcgtcggcg cccactcgtc
     1681 gcgggatgac gcggggtcaa cgtgtgggcc gacgtcgatc tgcagtagct cgccggcgcc
     1741 gagcggttgg aactggatgt ccgcgacgac ttcgccgacg gcgtagttcc cgccgcggtc
     1801 cttttggcag cggagcgtcg cccgtccggc cttgtggcgg gagaacggga tcagggggnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn ncgtcggggt ccttcttgac gtggtcgagg aggaggatgg cggcgccgcc
     1981 ggatgcttgg atgagccagc ggccgacgtt ctgttgccag cggtcgacgt cttgggcgac
     2041 gttctcgtcg agcccttcgg cggtgagcgc gccgacgaac gcgtcgatga cgatcaggtc
     2101 cgggttgagc cggtcgaaca cggcgttcat cgcggccttc tctgctggcc cgaagctgcc
     2161 ttcgggctgg acgtagaaga accggtcggc gacgtggcgg gggttcgcct tgagggcgag
     2221 gagccggttg acgatagcga tctcggagtc ctcgtagtcg acgtagacga cagcaccgtg
     2281 ctggaggact tcgagcgccc actggagtgc gagccacgac ttgcctgacg tcgacnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnctcgac ggtgatctgg tcgaggtcac cgcgtgctcg ggccagcgcg
     2521 gcggcgacgt catctggtgc gctgtaaccg aggtcggcga tcgcagcgcc ggccgcgatg
     2581 atgtcgcgca gctgccaggt gttgttgacg atccgcgcgt acgcgggcca ctggccgatg
     2641 gacggcgtgg tcgagatgag atcgacgagg ccgtcgaggt cgccgacctc atcgagcagg
     2701 ccatcacgac gaagttggtc agcgaccgtg acgacgtcca cggtgccgct ctcgagcttg
     2761 ccgatggcgg cggcgatgaa ctggtgcgct ggcttgtaga acctggcggg atcggggagc
     2821 ccagcggcag cgtcagcgtt gaggagcgcg gcaccgagcg cggcagcttc ggctgcgagg
     2881 tcgtgtggag gaacgcgagc gtcagcgcgg cttcgattcg atttgggcgg gtgatggtcc
     2941 ttggcgggca cactggtcac cgcccgtggg agtggtcgag gcagacgaac gtgtcgtcgg
     3001 cgcggcgggc gatcaggtca ccgacggtgg tgtagcagcc gcacgcgagg cggccggagt
     3061 agcgcgcggc gaaccattgg acgatccggt gggcttcggc ttcttcgcgg gcggcgcgcc
     3121 cgttgcagag cacgcaggcg ctgcgcgggc cgagaccgtg gatgcactcg tcaggatcgt
     3181 ggtcgtggtc ggtgctcatc gcgcggcccc gaacagggag gcttggccgg gcatctcggg
     3241 tcgggtgccg tacagggcgc ggcgacactc gtccatccgc gctcgcagga ggagctcgtt
     3301 gcgggggtcg aggtcgattc cgattgcgtg gcgtccgtgt aactcggcga cggcgagggt
     3361 ggtgccggtc ccgcagaacg ggtcgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng cgcgtcggag agtcctgcgg
     3601 cgtcggcaat cgcgactgcg cggcgggtct gcgggcggtt gctgccgcgg aaggcatcaa
     3661 gggtgtacgc gccttcgatg cgccgacgcg gttcaccgca ggcggcgcac acctcggccg
     3721 ggcacatcga caggatgaga cgctcggcga gcttcggggg ccacatcgca tagtgcgcga
     3781 ggcgtgacgg ttggctggac atggtccatg tcaggtgttc gtcgatctcg tcggaccagc
     3841 aatccagcgg cggcgcgcct gccgtggttg gggcgccgta gtcataggtc gccgtcatcg
     3901 tcccctcggg gcccgacttg acgctgtgtc cgtctcggga tgcacggcgc tccgtttgcc
     3961 gtcgcaccgc cgataggtcg aaccatcgtt gggtgtcgcg ggtggcgacc acgatgtacg
     4021 aggtggaggg acggaacttg tcgcccaacg cccccacagt cggattcggg cggtgccaca
     4081 cgatgatgtt gcggaccagc cactgtccgg ccggggactc tgcgccgctg atcgggttgc
     4141 gtccgtaggc gagacccatc gcgtacgcct gcgggatcag cgccagcgac ttccggtgcg
     4201 gccacgcgtg ccgagcgatc tgcttggcgc gccaatgtgc cgcattcgac tcgcgcatcg
     4261 aggcatccga accagcgaac tgctgctgcc cctcgcgcag tccaccaggc aggaggtcgc
     4321 cgccaccacc acctgacccg gaataggtgt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnccgccac
     4621 atccgaacca cgacagcgcc cacaccccac gcgcacgcga gcgccagggt cacgtgcccg
     4681 tacgtcaccg tcaacgcgaa cacagccact ccaccgacga cgcacgcgtc gcgtcccact
     4741 gatattccgc ccacggtgcg tcgagcccga ggccgacccg gccgaacccg cgcagatgcc
     4801 acgcgtccgc ctcgtcatca ttcgccgggc cggccacacc aaggtgatgg caccgcgacc
     4861 ccgccgcgtg cgtcatcacc gtcttgtccg ccgacgccga accagtcgcg aacatcttga
     4921 gatgcgcgtt gtgcacctcg acgaactcga tccccgactc agcaagccac acgcgcagaa
     4981 cgccgcggag ctcggtcaac gttgagcgaa cgccgcctcg tagacgacca gcgtcggccg
     5041 cggcgggttc aggctgacga tccgggccag ccgtgaccgc agctgcaggc accgcagcgc
     5101 gaggtcccag ggcgacggat gcgcaggcaa ccggtccgcg gtcacgctgt gcatcgaccc
     5161 gtcaggcgcg cagtagccga tccgtttcgt ggacgcgtcc agcccggcca cgtgttggtg
     5221 gtcgaaccgg acggtctggg ccgacgaggt cacagcacac caccaccctg gctgaaggtg
     5281 tgccgcggga cagcgcgtcc cgacggcagg tcggctgacg cgtcgtactg tggctga
//
LOCUS       DAORNF010000137         5334 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_11435_length_5334_cov_1.911726, whole genome shotgun sequence.
ACCESSION   DAORNF010000137 DAORNF010000000
VERSION     DAORNF010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 5334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5334
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11435_length_5334_cov_1.911726"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>211)
                     /locus_tag="PLV68_06890"
     CDS             complement(<1..>211)
                     /locus_tag="PLV68_06890"
                     /inference="COORDINATES: protein motif:HMM:NF012945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HRE01009.1"
                     /translation="ADAAGVDLLFEAAVAGGIPLIRALRESLRGEPIRRVLGIVNGTT
                     NFILTKMTEEGADYSVALAEAQALG"
     assembly_gap    212..294
                     /estimated_length=83
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<295..581)
                     /locus_tag="PLV68_06895"
     CDS             complement(<295..581)
                     /locus_tag="PLV68_06895"
                     /inference="COORDINATES: protein motif:HMM:NF015412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HRE01010.1"
                     /translation="MALQGNRVRIGVLGCGNVGASFVQLVGQQADAIEARTGIRLEVS
                     RVAVRSVARERDVQLPEGVLVRDAHAVVDDAGVDLVVEVIGGIEPARELITT"
     assembly_gap    638..677
                     /estimated_length=40
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<678..3839)
                     /locus_tag="PLV68_06900"
     CDS             complement(<678..3839)
                     /locus_tag="PLV68_06900"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HRE01011.1"
                     /translation="MRRAIMVITAMLASLVTFAVGADVALAHHSEISASVDCANTISW
                     TATSWSTGDSGTNNKVKVWYTTSSSSTQVTLTDTGKFNASNSYKFSGTLAWPGSVTSV
                     TIWVQEQVKWGANQNLAGPAGAASITVKKPTTCPGTPQPPTSTVTCASATSATVVVRF
                     GSSAGGSSSAYAITSPTTNVSPSSFTIAAAGGTRDVTFSNVGNGNLTVKYKINNVQKT
                     DYVVPVDCSPKPQVTFTTRCANAKGEVQISFTNSGKTSVTLTVYIDGAAQSPTVVVPA
                     GTTTPITKTYSGLSNNTTHTIEVKNGSTSVGSTTYKPECAPANPAVSFDKVCSNSSGS
                     VTISFTNDGNAAVTLDVYIDGVKQTPGVSVPARTTTAITKTYGGLSNNTTHTIEVKNG
                     STSVGSTTYKPECAPGTPVVTFTPVCSNSLGSVTISFTNSGNQAVSLDVFVDGAKNET
                     VNVPALTTTAITKTYGGLSNTTHTIEVKNGSNLVGSTTYKPECAPANPAVTFTPVCAN
                     SLGSVTIGFTNSGNAAVTLDVYIDNAKVDSIAVPGLTSTGLTRTYGNLANDPSHTIEV
                     RNGSTVVGSATYDPECAPGTGTATAVAVCANFDGTVTLTLTNTGGDLPVVFVVDGITY
                     TINKGDAPVQLTFNDVPDGTFAKTVTINGVDQVVTADVHCDAVLTALPECNEVDVDNN
                     ITAYIATITNPGATPVPVTWADGSGIVPAGGSLRIRSATTPIVVRNGDVTAATVAASS
                     EYCGTPVDFTKQLKGQPAAAETYSVKVSRLVGAEYVEAITFTINAGETKQFKLPSTVD
                     GPGVTYKVEEINAGSASLRTVSPDSFTLSGNLGETVSVVITNGYASVDIVKQVSATTV
                     LPGQVLTYTLTATNTGGLTLNPAVITDILPPQVSMQSALVADGKGTCVLSRDTRPELV
                     TCTLAAPLAPGAVAPVITIVTLVDAGVPANTVLVNQAKVVGAFTEPPSTITEPGIESL
                     SCLPAIDATVCDLSAAVNTTVGEQSESLPPDTPATTQPPAPTMPPDSRLPETGSSGTL
                     PMGGIALILIGLGGVAFVIRRRPA"
     regulatory      complement(3933..4018)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            complement(4206..4871)
                     /locus_tag="PLV68_06905"
     CDS             complement(4206..4871)
                     /locus_tag="PLV68_06905"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HRE01012.1"
                     /translation="MASKIGAVFQRLLSGTRQAARATRDQHDERTSTGGSSRRTKRQK
                     STAQPTPKAPAKPDAKQESRPQPKPGVPPPTSGTGQPRGADESTSSRNGVPGAPSRST
                     SLAGARIEYNPDLDGDADPGEVVWTWVPYEEDATQGKDRPVLIVGRRNGWLIGVMLTS
                     KAHDGDPDHVSVGTGAWDRDGRVSYAKLDRVLEVDAGQVRREGAILPRARFDAVIAEL
                     LAS"
     gene            complement(4918..>5334)
                     /locus_tag="PLV68_06910"
     CDS             complement(4918..>5334)
                     /locus_tag="PLV68_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HRE01013.1"
                     /translation="VGAAAITVYTVGTIKHIPGVRTYVAVDGGMSDNPRPVLYGSGYE
                     AFLPRDVGAARDRSVRLVGKHCESGDVLIFDAAVPNSLAVGDLLATPVTGAYGHSMGS
                     NYNKILRPPVVFVADGDARLVVRRETYDDLLACDLG"
BASE COUNT          813 a         1734 c         1722 g          942 t
ORIGIN      
        1 aaacccagtg cctgcgcctc ggcgagcgcc accgagtagt cggcaccctc ttcggtcatc
       61 ttggtgagga tgaagttggt ggtgccgttg acgatgccga gcactcgccg gatcggctcg
      121 ccgcgcaggc tctcgcgtag cgccctgatc agcgggatgc caccggccac cgcggcttcg
      181 aacagcaggt cgacaccggc cgcatcggcg gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngtggtg
      301 atcaactcac gcgccggttc gatgccgccg atcacttcga ccaccagatc gaccccggcg
      361 tcgtcgacca ccgcatgggc gtcgcgcacc agcacgccct cgggcaactg cacgtcacgc
      421 tcgcgcgcca cgctgcgcac ggccacccgg ctcacctcca gacggatgcc ggtgcgggcc
      481 tcgatcgcgt cggcctgctg accgacgagc tgcacgaacg atgcgccgac gttgccgcaa
      541 ccgagcacgc caatccggac acgattcccc tggagagcca cgggcacgag gctacgcagt
      601 cgcgccgaag ctccgaaacc cgtttggaca cgggtttnnn nnnnnnnnnn nnnnnnnnnn
      661 nnnnnnnnnn nnnnnnnggc gggccggcgg cggatgacga aggccacgcc gccgagaccg
      721 atcaggatca gtgcgattcc acccatcggc aacgtgccac tcgacccggt ctcgggcagg
      781 cggctgtccg gaggcatcgt cggcgcaggt ggctgcgtgg tcgccggggt gtccggaggc
      841 aggctctcgc tctgttcccc gaccgtggtg ttgaccgcgg ccgacagatc gcacaccgtg
      901 gcgtcgatgg cgggcaagca gctcagcgac tcgatgcccg gctcggtgat ggtgctgggc
      961 ggctcggtga aagcgcccac gaccttggcc tggttgacca gcaccgtgtt ggccggcacc
     1021 ccggcatcga ccagggtgac aattgtgatc accggcgcca ccgcgcctgg ggcgagcggt
     1081 gcggccaacg tgcacgtgac gagctccggg cgtgtgtcac gagacagcac gcacgtgccc
     1141 ttgccatcgg ccacgagtgc cgactgcatc gacacctgcg gcggcaggat gtcggtgatc
     1201 accgccgggt tcaacgtcaa cccgccggtg ttggtcgcgg tcagggtgta ggtcaggacc
     1261 tggcccggca acaccgtcgt cgccgagacc tgcttgacga tgtcgaccga ggcgtagcca
     1321 ttggtgatca cgacactcac cgtctcaccg aggttgccgc tcagcgtgaa gctgtcgggc
     1381 gacacggtgc gcagcgaggc cgagccggcg ttgatctcct ccaccttgta ggtcacacct
     1441 ggaccatcga ccgtcgaggg cagcttgaac tgcttggtct cgccggcgtt gatggtgaag
     1501 gtgatcgcct cgacgtactc cgcaccgacc aggcgcgaca ccttgaccga gtaggtctcg
     1561 gcggccgcag gctggccctt caactgcttg gtgaagtcga ccggtgtgcc gcagtactcg
     1621 ctggacgcgg cgaccgtggc cgccgtgacg tcgccgttgc gtaccacgat cggcgtcgtc
     1681 gcactcctga tgcgcagcga cccgccggcg ggaacgatgc ctgaaccgtc ggcccaggtg
     1741 accggcaccg gggtggcgcc tgggttggtg atggtggcga tgtacgccgt gatgttgttg
     1801 tcgacgtcga cctcgttgca ctcgggcaac gccgtcagca ccgcgtcaca gtgcacgtcg
     1861 gcggtcacga cctggtcgac gccgttgatc gtcacggtct tggcgaacgt gccgtccgga
     1921 acatcgttga aggtcagctg cactggagcg tcgcccttgt tgatggtgta cgtgatgccg
     1981 tcgaccacga acaccacagg caggtcgcca ccggtgttgg tcagcgtcag cgtgaccgtg
     2041 ccgtcgaagt tggcgcacac cgcgaccgcc gttgccgtgc cagtaccggg ggcgcactcg
     2101 gggtcgtagg tggcagagcc gaccacggtc gagccgttgc gcacctcgat cgtgtggctc
     2161 gggtcgttgg ccaggttgcc gtacgtgcgg gtcaggcccg tgctggtcaa gccaggaacg
     2221 gcgatcgagt cgaccttggc gttgtcgatg tagacgtcga gggtgaccgc cgcgttgccg
     2281 ctgttggtga agccgatcgt caccgaaccc aacgagttcg cacacaccgg ggtgaacgtg
     2341 accgccgggt tggccggggc gcattcgggc ttgtatgtgg tggagcccac gaggttcgag
     2401 ccgttcttga cctcgatcgt gtgggtggtg ttgctcaacc cgccataggt cttggtgatc
     2461 gccgtcgtcg tcaaggcagg gacgttgacc gtctcgttct tcgcaccgtc gacgaacacg
     2521 tccaacgaca ccgcctggtt accgctgttg gtgaagctga tcgtcaccga acccaacgag
     2581 ttggagcaca ctggggtgaa cgtgaccacg ggagtgcccg gggcgcattc gggcttgtag
     2641 gtggtcgaac cgaccgaggt cgaaccgttc ttgacctcga tcgtgtgggt cgtattgttg
     2701 ctcaacccgc cataggtctt ggtgatcgcc gtcgtggtcc gagccggaac actgacgccg
     2761 ggcgtctgct tcacaccgtc gatgtacacg tccagcgtga ccgccgcgtt gccatcgttg
     2821 gtgaagctga tcgtcaccga acccgacgag ttcgaacaca ccttgtcgaa cgacacagcc
     2881 ggattggccg gagcacactc aggcttgtac gtggtcgaac cgaccgaggt cgaaccgttc
     2941 ttgacctcga tcgtgtgggt cgtgttgttg ctgagaccgc tgtaggtctt ggtgatgggc
     3001 gttgtcgttc cggccggaac caccaccgta gggctctgcg ccgcgccatc gatgtagaca
     3061 gtcagcgtca ccgaggtctt gccgctgttg gtgaaggaga tctgcacctc gcccttcgcg
     3121 ttggcgcatc gggtggtgaa ggtgacctgt ggcttgggcg agcagtcgac cggcacgacg
     3181 tagtcggtct tctgcacgtt gttgatcttg tacttgacgg tcaggttgcc gttaccgacg
     3241 ttgctgaacg tgacgtcgcg agtgccaccg gccgcagcga tggtgaacga gctcggcgac
     3301 acgttcgtgg tcggcgatgt gatggcgtaa gccgacgacg aaccaccagc gctcgatccg
     3361 aaccgcacta ccaccgtggc cgacgtggcc gaggcgcacg tcaccgtcga tgtgggcggc
     3421 tgtggagtgc cggggcaggt ggtcggcttc ttgaccgtga tcgacgcggc ccccgccggg
     3481 ccggcgaggt tttggttcgc tccccacttg acctgctcct gaacccagat cgtcaccgac
     3541 gtgacgctgc cgggccaggc cagggtgccg ctgaacttgt agctgttcga cgcgttgaac
     3601 ttgccggtgt cggtcagcgt cacctgggtg ctgctggaac tggtggtgta ccagaccttg
     3661 accttgttgt tggtaccgct gtctccggtc gaccacgagg tcgcggtcca cgagatcgtg
     3721 ttggcacaat ccaccgacgc gctgatctcc gagtggtggg ccagcgccac gtcagcaccg
     3781 accgcgaacg tcaccaacga tgcgagcatc gccgtgatca ccatgatggc tcggcgcatg
     3841 gagcgccgag cattgacccg tccagtcatt ctgttgtctg cccttcttcg acccagcgaa
     3901 ctgggtgctc accaccacac cgtcttgagg tggcattcga aggtcgggtt cgccatttgc
     3961 tcggctacgg acttaggcga ccaggtacac ccgcagcgtc attgcatccc tcgtgcacct
     4021 cggatggtaa gcgtggctgc actgcatgtg caaagaacct gccccgaatc gcgctccaat
     4081 ggtccgaacg acctaccgca ggctcaccaa cccctgccca atccctgaca gacccaccgt
     4141 cagtgttgac agctccacgc tccgcggcca acacctgaag agatcttgcc gggtcgctcc
     4201 gtgacctacg aggccaggag ctcggcgatc actgcgtcga agcgggctcg cggcaggatc
     4261 gcgccttcac ggcgcacctg gccggcatcg acctcgagga cccgatcgag cttggcgtag
     4321 ctgacgcggc cgtcgcgatc ccacgccccg gtgccgacgc tgacgtggtc gggatcgccg
     4381 tcgtgggcct ttgacgtcag catcacgccg atcagccagc cattgcgccg acccacgatc
     4441 aacacggggc ggtccttacc ctgcgtcgcg tcctcctcgt agggcaccca cgtccacacc
     4501 acctcgccgg ggtcggcatc accgtcgaga tcggggttgt actcgatccg cgccccggcc
     4561 aacgacgtgc tccgcgacgg cgcccccggc acaccgttac ggctcgacgt cgactcatcg
     4621 gctccccgcg gttggcccgt cccgcttgtt ggcggcggca cgcctggctt cggctgcggg
     4681 cgcgactcct gcttcgcgtc cggtttcgcc ggggccttcg gcgttggctg ggctgtcgat
     4741 ttctggcgct tggtgcgccg cgacgacccg cccgtcgacg tccgttcgtc gtgctggtcg
     4801 cgcgtcgcgc gcgctgcctg gcgtgtcccg ctcagcaacc gttggaacac cgccccgatc
     4861 ttgctcgcca tgacgcggca atctaacggc cgacgtcccg ccgaccgccc ttcccgttca
     4921 gcccaagtcg caggcgagca gatcgtcgta ggtctcgcgg cgcacgacca ggcgggcgtc
     4981 gccatcggcc acgaacacca ccggcgggcg caggatcttg ttgtagttgc tgcccatgct
     5041 gtggccgtac gcgccggtga ccggcgtggc gagcaggtcg cccacagcca acgaattggg
     5101 aacggcggcg tcgaaaatca gtacgtcgcc gctctcgcag tgcttgccga ccagccgcac
     5161 cgaccggtcg cgcgcggcac ccacgtcacg cggcaggaag gcctcgtagc cgctgccgta
     5221 gagcaccggg cgggggttgt cgctcatgcc gccgtcgacc gcgacgtacg tgcgcacacc
     5281 gggaatgtgc ttgatggtgc cgaccgtgta cacggtgatc gcagccgcgc cgac
//
LOCUS       DAORNF010000138         5332 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_11441_length_5332_cov_1.523214, whole genome shotgun sequence.
ACCESSION   DAORNF010000138 DAORNF010000000
VERSION     DAORNF010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 5332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5332
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11441_length_5332_cov_1.523214"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="PLV68_06915"
     CDS             <1..372
                     /locus_tag="PLV68_06915"
                     /inference="COORDINATES: protein motif:HMM:NF014872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01014.1"
                     /translation="ERSEATLSEIVRWRRENQPGGQNAGSVFVNPIPGELSAGELVDR
                     VGLRGLRLGTAAVSDKHANFIQGSEGGRAADVLELIEVVRAKVLASTGFALRSEVRLV
                     GFDGVEPDDNPGVLVEPGGDR"
     gene            369..>695
                     /locus_tag="PLV68_06920"
     CDS             369..>695
                     /locus_tag="PLV68_06920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01015.1"
                     /translation="MSEDDLPVVPLGDAPVPDKVLEELLAAFAGDEDLATDEMPIVPD
                     VPTAGQRVDLDDPAIDALLGITADEPAAPAPDETTLGEPAHDGRAVDGPAVEQLPGDD
                     QAIVVTA"
     assembly_gap    696..740
                     /estimated_length=45
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <741..1831
                     /locus_tag="PLV68_06925"
     CDS             <741..1831
                     /locus_tag="PLV68_06925"
                     /inference="COORDINATES: protein motif:HMM:NF015739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HRE01016.1"
                     /translation="ADPADPEPVVLNPILLDPGLAEPASPPSSPGEAEAGEAAPAGPL
                     APPARRTIRIGGDDDGAMDPVYLDEEGAGRLRGETDRQTEASVERTTIFIGDDDLDGA
                     SGGIPIATGSASIDPRLRARRIAVKRALGRKRLKWVVLAVVVVLLITSVLALLGSSLF
                     RVQQIEVSGRSGTTNEAIEAAVAELKDTPVLLVDTHAIEEQLERSPWIREARVATDFP
                     HGASIEVVERVPLATYPGTDGRWRIIDVEGVVLTVIANRPLAFMEIEGPGADAEAGGS
                     AGQALRHAAELVEALTPAVRSRTQKVLVDPTGELRLQFADTTVELGAAENLLDKLTWL
                     EVVIKSGRVDDCSVVNVTTRDVGCVPAR"
     gene            1938..3035
                     /gene="ftsZ"
                     /locus_tag="PLV68_06930"
     CDS             1938..3035
                     /gene="ftsZ"
                     /locus_tag="PLV68_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HRE01017.1"
                     /translation="MAGAPQNYLAMIKVIGVGGGGVNAVNRMIDAGLKGVEFIAVNTD
                     AQALLMSDADVKLDIGRELTRGLGAGSDPEVGRQAGESHADEIEEMIKGADMVFITAG
                     EGGGTGTGAAPVIAEIAKKMGALTIGVVTRPFSFEGRRRSVQADLGVTKLKEKVDTLI
                     VIPNDRLLTVANEKTSVLNAFKMADEVLLQGVSGITNLITTPGLINTDFADVRMVLSN
                     AGSALMGIGQASGENRAVTAAKAAISSPLLEAAIDGARGILLNITGPSSMGLFEMNAA
                     AEIIHGVAHQDANIIFGTVIDDEMGDEVKVTVVAAGFDRWDAQPASSGRAAATVIETA
                     KPPAPIKDLFATDAPDPLDDDDDFDVPSFLK"
     assembly_gap    3183..3317
                     /estimated_length=135
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3358..>3624
                     /locus_tag="PLV68_06935"
     CDS             3358..>3624
                     /locus_tag="PLV68_06935"
                     /inference="COORDINATES: protein motif:HMM:NF016363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HRE01018.1"
                     /translation="MQPRPSNPRPAVAPVRAVPPAGNEPATVKPRRFDQAQEIADRFK
                     EGLPVIMNLESADREVARRLIDFASGICYGLNGSMEKVASGVYLL"
     assembly_gap    3625..3756
                     /estimated_length=132
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3757..>4056
                     /locus_tag="PLV68_06940"
     CDS             <3757..>4056
                     /locus_tag="PLV68_06940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01019.1"
                     /translation="KGYDPVEVREYLESLSGSIEGLQSQATAMEARARAAVARLQELN
                     ASQHAGGQADVKRTDPTPAKGAPLAEVAEAETISRTLLLAQRTADHTVAEAKAEAA"
     assembly_gap    4057..4244
                     /estimated_length=188
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4245..4664
                     /locus_tag="PLV68_06945"
     CDS             <4245..4664
                     /locus_tag="PLV68_06945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01020.1"
                     /translation="ISDVDHLELHIITQRDRLRDVAAALTEMAERVPGGLGEIRRPLV
                     SAVEPTANGRASESGGSDPVLDAIASSAAVSSVGHADDGDVGERDLGERDDDMGDWDA
                     GEQDPDRSAQADDHGAEPLPFGDITAEVPRVDGDELR"
     assembly_gap    4817..4826
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4849..>5332
                     /locus_tag="PLV68_06950"
     CDS             4849..>5332
                     /locus_tag="PLV68_06950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01021.1"
                     /translation="MAKRAATPAAGQKASGTAAPAAQTGAPPTKTGTAASAAKAGTVA
                     AKKASSAKSDKSVRAKSAKSAPPATKTATNIETKTATKGATKTVTQTGTKTTTKAAAK
                     QADVASAKQADEVVAETVEPAPKVVVKAAAPAAKPERVVVPIPPPKGVTKDGISYTKD
                     F"
BASE COUNT          850 a         1643 c         1637 g          692 t
ORIGIN      
        1 gaacgctccg aagcgacgct gagcgagatc gtccgctggc ggcgggagaa ccagccggga
       61 gggcagaacg ccggatccgt gttcgtcaac cccatcccgg gcgagctctc cgcgggtgag
      121 ctggtcgacc gggtcggcct gcgcggtctg cgcctgggca cggccgcggt atccgacaag
      181 cacgccaact tcatccaggg ctccgagggc ggtcgtgccg ccgacgtgct ggagttgatc
      241 gaggtggtgc gggccaaggt gctggccagc acgggcttcg cgctgcgcag cgaggtgcgc
      301 ctggtcggct tcgacggcgt cgagcccgac gacaacccgg gtgtgctcgt cgaaccgggt
      361 ggtgatcgat gagcgaggac gatctgccgg tggtgccact gggcgatgcg ccggtgcccg
      421 acaaggtctt ggaggagctc ctcgccgcct ttgccggcga cgaggacctg gcgaccgacg
      481 agatgccgat cgtgcccgac gtgcccaccg ccggtcagcg ggtcgacctc gacgacccgg
      541 cgatcgacgc actcctgggc atcacggccg atgaacccgc agcgccggcg ccggacgaga
      601 caacactcgg tgagccagca cacgacgggc gagcggtcga cgggccagcg gtcgagcagt
      661 tgcctggcga cgatcaggcc atcgtcgtca ccgcannnnn nnnnnnnnnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn cagccgatcc ggctgatccg gaaccagtcg tgctgaaccc
      781 gatcctgctg gaccccgggc tggccgaacc cgcctctcca ccctcatcgc ctggcgaggc
      841 ggaggccggt gaggcagcgc cggccggccc gctggcacca ccggcgcggc gcacgatccg
      901 catcggcggc gacgacgacg gggcgatgga cccggtctac ctcgacgaag aaggcgccgg
      961 acggttgcgc ggtgagaccg accgccagac cgaggcgtcg gtcgagcgga ccacgatctt
     1021 catcggcgat gacgatctcg acggcgcctc cggcggcatc cccatcgcta ccggctcggc
     1081 cagcatcgac cctcgtctgc gcgccaggcg tatcgccgtc aagcgcgccc tcggacgcaa
     1141 gcggctgaag tgggtggtgt tggcggtggt cgtcgtgctg ctgatcacct cggtgttggc
     1201 cctgctcggt tcgtcgttgt tccgcgtgca acagatcgag gtcagcggcc gctcgggaac
     1261 gacgaacgag gcgatcgagg ctgcggtagc ggaactgaag gacaccccgg tactgctggt
     1321 cgacacccac gcgatcgaag aacagctcga acgcagcccg tggatccgtg aggcgcgcgt
     1381 ggcaaccgac ttccctcacg gcgcgtcgat cgaagtggtc gagcgggtgc cgttggcgac
     1441 ctatcccggc accgacggcc gatggcggat catcgacgtc gagggcgtgg tgctgaccgt
     1501 gatcgccaac cgccccctgg cgttcatgga gatcgaaggc ccgggggccg atgccgaggc
     1561 tggtggttcg gccggtcagg cactgcgcca cgccgccgaa ttggtcgagg cgctgacccc
     1621 cgcggtgcgg tcgcgcacgc agaaggtgct ggtcgacccc accggtgagc tgcggctgca
     1681 gttcgccgac accacggtcg aactcggcgc ggctgagaac ctgctcgaca agctgacctg
     1741 gctggaagtg gtcatcaaga gcggtcgggt cgacgactgt tcggtcgtca atgtgaccac
     1801 ccgtgacgtc ggatgtgttc ccgcacggtg actgtccgca gaaagattgt tgcgacgtgg
     1861 caggatgtgc gagcaatgca cgtggttcgg cctagcctgc catgcactcg taggaaatgg
     1921 atgtcggagg tcccctgatg gcaggcgcac cacagaacta tctcgcaatg atcaaggtga
     1981 tcggtgttgg cggtggcggc gtcaatgccg tcaaccgcat gatcgacgcg gggctgaagg
     2041 gcgtcgagtt catcgcagtc aacaccgacg ctcaagcgct gttgatgagc gatgccgacg
     2101 tcaagctcga catcggccgc gagctcactc gcggtctcgg tgcggggagc gaccccgagg
     2161 tcggccgtca ggccggcgag tcgcacgccg acgagatcga agagatgatc aaaggcgccg
     2221 acatggtgtt catcacagcg ggtgaggggg gtggcaccgg taccggagcc gcgccggtca
     2281 tcgccgagat cgccaagaag atgggtgcgc tcacgatcgg ggtggtcacc cgtccgttct
     2341 ccttcgaggg ccgccgccgt tcggtgcagg ccgacttggg cgtcaccaag ttgaaggaga
     2401 aggtcgacac cctcatcgtc atccccaacg accgcttgct caccgtcgcc aacgagaaga
     2461 ccagcgtgct caacgcgttc aagatggccg acgaggtgct gttgcagggt gtgtcgggca
     2521 tcaccaacct gatcacgacc cccggcctca tcaacaccga cttcgccgac gtgcggatgg
     2581 tgctctccaa tgccggctcg gcgttgatgg gcatcggcca ggccagcggc gagaaccgcg
     2641 ccgtcacggc ggccaaggcc gccatcagca gcccgctgct cgaagcggcg atcgacgggg
     2701 cccgcggcat cctgctcaat atcacgggtc cgtccagcat gggcctgttc gagatgaacg
     2761 ctgccgccga gatcatccac ggggtcgccc accaggacgc caacatcatc ttcggtaccg
     2821 tcatcgacga cgagatgggc gacgaggtca aggtcaccgt cgtcgccgcg gggttcgacc
     2881 gctgggatgc tcagccggcc tccagcgggc gggccgcggc cacggtcatc gagaccgcca
     2941 agcctcctgc gccgatcaag gatctgttcg ccaccgacgc gcccgatccg ctcgacgacg
     3001 atgacgactt cgacgtcccg tcgttcttga agtagccgcc ctgtcctcga gacctccatc
     3061 ggatagcctc acgccaggag ggtttggtcg atgtccatca tgaagcgagc catggatttc
     3121 ttgggtctcg gtcccgacga cgcgtacgac gactacgacg cgccgtacga gcccgagcgc
     3181 cannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnccc ggcccgccgt ccgcagagcc caggcgacga ttcgtccgtg
     3361 cagccgcggc ccagcaaccc gcgtcctgcg gtcgcgcccg tgcgcgccgt gccgcccgct
     3421 ggcaacgagc ccgccacggt caagccgcgc cggttcgatc aggcccaaga gatcgccgac
     3481 cggttcaagg aaggcctacc ggtcatcatg aacctcgaga gcgccgaccg cgaggtcgcc
     3541 cgccgtctga tcgacttcgc cagcggcatc tgctacgggc tcaacggcag catggagaag
     3601 gtcgccagcg gcgtctacct gctcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnaagg gttacgaccc tgtcgaggtg
     3781 cgtgagtacc tcgagtcgtt gtccggctcg atcgaaggtt tgcagtcgca ggcaaccgcg
     3841 atggaggcgc gtgcccgggc cgctgttgca cgtctgcaag agctgaacgc gtcgcagcac
     3901 gccggcggcc aggccgacgt gaagcggacc gacccgaccc cggccaaggg cgcaccgctg
     3961 gccgaggtgg ccgaggccga gacgatcagc cgcaccctgc tgctggccca gcgcaccgcg
     4021 gaccacaccg tcgccgaggc caaggccgag gccgcgnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnatcagc gacgtcgatc
     4261 acctcgaact gcacatcatc acccagcgcg atcgcctgcg cgacgtcgcc gcggccttga
     4321 ccgagatggc cgagcgggtg ccgggcggtc tcggcgagat ccgtcgcccg ctggtgtcgg
     4381 cggtcgaacc caccgccaac gggcgcgcat ccgagtccgg tgggagcgac ccggtgctcg
     4441 atgcaatcgc ctcgtcggca gcggtgagtt cggtcggcca tgccgacgac ggtgacgtcg
     4501 gcgagaggga cctcggcgaa cgtgacgacg acatgggcga ctgggacgcg ggtgaacagg
     4561 acccggaccg ctccgcccag gccgacgacc acggtgccga gccactcccg ttcggcgaca
     4621 tcaccgccga ggtgccgcgc gtcgacggtg acgaactgcg ctagctcttc tgtccgaaag
     4681 ttgtcgcgtc gggttgcgtg actccggcgc cgttcacggc aattcctggg tatgccgttg
     4741 gtgaacgccc ggccatgccc tacgatccgc acgttcatcg accgtcatcc gaacaccccg
     4801 ctggccgatc gcctgcnnnn nnnnnncatc gaccgtggag gtttcctcat ggccaaacga
     4861 gctgccacac cagccgcggg acagaaggca tccggcaccg ccgcaccggc cgcgcaaacc
     4921 ggcgcgccgc ccaccaagac cggcaccgcc gcatctgcgg ccaaagcggg cacagttgct
     4981 gccaagaagg catcgagcgc caagagcgac aagagcgtgc gagccaagag cgccaagtct
     5041 gctccgccag caaccaagac cgcgaccaac atcgagacca agactgcgac caagggagcc
     5101 accaagaccg tgacacagac cgggaccaag accacgacca aggctgctgc caagcaagcc
     5161 gacgttgctt ccgccaagca agccgacgag gtcgtggcgg aaaccgtcga gccggcaccc
     5221 aaggtggtgg tcaaggctgc cgctccggcg gccaagccag agcgggtcgt cgtgccgatc
     5281 ccgcctccga agggcgtcac caaggacggc atctcgtaca cgaaggactt cg
//
LOCUS       DAORNF010000139         5256 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_11654_length_5256_cov_1.661604, whole genome shotgun sequence.
ACCESSION   DAORNF010000139 DAORNF010000000
VERSION     DAORNF010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 5256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5256
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11654_length_5256_cov_1.661604"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="PLV68_06955"
     CDS             <1..188
                     /locus_tag="PLV68_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01022.1"
                     /translation="RQAQAGAGGSAVLALEHAAAATPPASTVAIIRRCADARRRVAAV
                     TVRDGAVGMRDAPYPLQ"
     gene            236..976
                     /locus_tag="PLV68_06960"
     CDS             236..976
                     /locus_tag="PLV68_06960"
                     /inference="COORDINATES: protein motif:HMM:NF015658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HRE01023.1"
                     /translation="MSHELDKATATPDTDTDIDDNTGTDGNTTITDADDVRVLPWYAN
                     PINIGALLIAVLVLAGVGGFVIGERNATERANDVDRGFLQDMRAHHEQAVEMSMIVLL
                     KNGIGDDGVFDPDLAVTGGVKTVAQSIIVGQMLEIGRMVQMLRYVGADEANTTGTAMA
                     WMNQAVPLERMPGMASEADLDTLFAAQGADAATQFIDLMITHHEGGIAMAIYARDHAA
                     TDEVRTFAQGVIDGQQGEIHELEGLRGG"
     assembly_gap    1198..1337
                     /estimated_length=140
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1338..2670
                     /locus_tag="PLV68_06965"
     CDS             <1338..2670
                     /locus_tag="PLV68_06965"
                     /inference="COORDINATES: protein motif:HMM:NF016143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HRE01024.1"
                     /translation="GTNKEPQPYDEYLQRALADVQDFWRATYPAVYGSTYEELAGGIW
                     ASYPGREGNPIPAYPDGCRGDPREMYIAQSNAFYCSFGDYMAYDDFDLIPRLVDDYGE
                     TAVGVVFAHEFGHAIQARVNNPDVWGVNAPVVRGEQQADCFAGAWTAHVARGESALLN
                     FGDEGVRAGLSAMVAVKDPLLVLDANGQPVSPDVFEGSAHGTAFDRVGAFQYGFLGGA
                     PACKDMETTPLPLLNIKFTDEGDLAREGNTPYDGDPAQQIDPLMTLLSTDLDRFWTLS
                     VSGFSSPQLVGYPNNGPYPSCPGVPEDNFPLHVFYCQATNQVMYDDGYTRRLFQVFGD
                     FSVGYLISNAWNEAVQTELGSSLAGERRLLINTCLTGAWTGNIIPDGTNTQPFTISPG
                     DLDEAVQVALVLSPEEAISDMSMGSSFEQIDFFRAGVLGGVSECNNRIVNS"
     gene            complement(2690..3661)
                     /locus_tag="PLV68_06970"
     CDS             complement(2690..3661)
                     /locus_tag="PLV68_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRE01025.1"
                     /translation="MSDGADPRDDLIEYDEFGLFHENIAEFGLAVPELPPVHRTHVEL
                     PDGRSLSALVWGEGPPRLVFLHGGAQNAHTWDTVLLALGVPAVAIDLPGHGHSGWRED
                     GGYNSDNMADDVAVAITALAPHAAAVVGMSMGGMTSIALASRHPELVRQLVVVDVTPG
                     VNPQKAKAIHDFVNGPQSFASFTDLLARTMEHNPTRSESSLRRGILHNAHRNADGSWQ
                     WNYDRRRQSRVAEPGDAGSEADAERSLAEQTAVMAQLWGALERIQSPIMLVRGMLSPV
                     VDDDDVAECRRRQPTIRVEPVDGAGHSVQGDRPVELAALIRDFVAGV"
     gene            complement(3789..>4865)
                     /locus_tag="PLV68_06975"
     CDS             complement(3789..>4865)
                     /locus_tag="PLV68_06975"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HRE01026.1"
                     /translation="LTVPDSVEDGVITTTVPAANKTAISRTLRQGVFGDDVKLIQTRL
                     TELGFAPGVADGAFGEQTKQAVWAYEKYVLQIPRADATGKVTPEMWQGMQDTITILPR
                     RPGSGTHVEVYLPEQVTIVFTDNKPTLIIHISSGEAMTRRDPWWCEMVQINEEKGVAL
                     AEPREEKRCGISFTPGGVFKFTRAVQGRRVSALGGMYDPIYFNYGIALHGAKEVPLEP
                     ASHGCIRMHMNISKYFQDYVHLKDLVYVWGQDGRQPEQYSKNESLPVFDKVDEEWRAE
                     QKSTTTTAAAAVTATSAKSQTTTTAAATPTTKPPVTTSAPTTTAAPATTAAPTTTAAP
                     TTVAPTTAAPTTTAAPTTAAPPSP"
     assembly_gap    4866..4900
                     /estimated_length=35
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4901..5167)
                     /locus_tag="PLV68_06980"
     CDS             complement(<4901..5167)
                     /locus_tag="PLV68_06980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01027.1"
                     /translation="MSNSGRAASGGSAQRRVANAKPRSAAGGAKSRGPAAGRWIPAVA
                     AIGVLLIVVLAGTLNGDSPKDAKAAGDTSTPAVIDQTNPSTGDDN"
BASE COUNT          820 a         1745 c         1667 g          849 t
ORIGIN      
        1 atcgacaggc ccaggccggt gccggtgggt cggccgtgct cgcgctggaa cacgccgctg
       61 cggcgacgcc tcctgcgagc acagtcgcga tcatccgccg atgcgccgac gcgcggcggc
      121 gcgtcgcggc ggtcacggtg agggacggcg cggtcggcat gcgtgacgca ccgtatccgc
      181 ttcagtgacc cctggccctg tggcatgtcc gcctgacgct gtcagactgg acctcgtgag
      241 ccacgaactt gacaaagcca cggcgacccc cgacaccgac accgacatcg acgacaacac
      301 cggcaccgat ggcaacacca ccatcaccga tgcggacgac gtgcgcgtgc tgccgtggta
      361 cgccaacccg atcaacatcg gtgcgctgct gatcgcggtg ttggtgctgg ccggcgtcgg
      421 cggcttcgtg atcggcgagc gcaacgccac cgagcgcgcc aacgacgtcg accgcggctt
      481 tctgcaagac atgcgtgccc accacgaaca agccgtcgag atgagcatga tcgtgctgct
      541 caagaacggc atcggcgacg acggcgtgtt cgaccccgac ctggccgtga cgggcggcgt
      601 caagaccgtc gcccagagca tcatcgtcgg ccagatgctg gagatcggac ggatggtgca
      661 gatgctgcgc tacgtcggtg ccgacgaggc caataccacc ggcaccgcga tggcgtggat
      721 gaaccaggcg gtgccgctcg agcggatgcc cggcatggcc tccgaagccg acctcgacac
      781 actgttcgct gcccagggcg ccgacgcggc gacgcagttc atcgacctga tgatcaccca
      841 ccacgaaggt ggcatcgcga tggcgatcta tgcccgcgat cacgccgcga ccgacgaggt
      901 gcgcacgttt gcccaaggtg tgatcgacgg ccagcaaggc gagatccacg aactcgaagg
      961 gctgcgcggg ggctgagcga ccaacatcgc ctcgccgggc gcgcaacact tcgacaacac
     1021 cgacctgaca gaaattgcca ggtggcgccg gcgacgtaag atcgcctcgt gcgccgatca
     1081 tccgctgcca gcgttgccgc catcgttgtc tctgctgccg tgctgggcgc atgtgccagc
     1141 accgatccgg gggtgacggc ccggcgggcc gactcggtga tccagatcga cattccgnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnncgg caccaacaaa gagccccagc catacgacga atacctccag
     1381 cgcgccctcg ccgacgtgca ggacttctgg cgggcgacgt atccggccgt gtatggcagc
     1441 acctacgaag agctcgcagg cggtatctgg gccagctacc ccggccgcga gggcaacccg
     1501 atcccagcgt atcccgacgg ctgccgcggc gacccacgcg agatgtacat cgcgcagtcc
     1561 aacgcgttct actgcagctt cggcgactac atggcctacg acgacttcga cctgatcccc
     1621 cgcctggtcg acgactacgg cgagactgcg gtgggcgtgg tgttcgccca cgagttcggc
     1681 cacgccatcc aggcccgcgt caacaaccct gatgtgtggg gcgtcaatgc tccggtcgtg
     1741 cgcggcgagc agcaagccga ctgcttcgcc ggagcatgga cggcgcatgt ggcgcgcggc
     1801 gagtcggcgc tgctcaactt cggcgacgag ggggtgcgtg ccgggctctc cgcgatggtg
     1861 gcggtcaagg acccgctgct ggtgctcgac gccaacggtc aaccggtgag ccccgacgtg
     1921 ttcgagggca gcgcccacgg caccgcgttc gaccgggtgg gtgcgttcca gtacggcttt
     1981 ctcggtgggg ccccggcgtg caaggacatg gagaccaccc cactgccgct gctgaacatc
     2041 aagttcaccg acgagggcga cctggcccgc gagggcaaca cgccctacga cggtgacccc
     2101 gcccagcaga tcgacccgct gatgaccctg ctctcgaccg acctcgatcg gttctggacg
     2161 ctgtcggtca gcggattcag ctcgccgcag ctcgtcgggt atcccaacaa cggcccgtac
     2221 ccaagctgcc ctggtgtgcc cgaggacaac ttcccgctcc acgtgttcta ctgccaggcc
     2281 accaaccagg tgatgtacga cgacgggtac acccgccgct tgttccaggt gttcggcgac
     2341 ttctcggtgg gctacctgat ctccaacgcg tggaacgagg cggtccagac cgagctcggc
     2401 agcagcctcg ccggcgaacg gcgcttgctg atcaacacct gcctcaccgg cgcctggacc
     2461 ggcaacatca tccccgacgg caccaacacg cagccgttca ccatctcgcc cggcgacctc
     2521 gacgaagccg tccaggtcgc gctggtgctg tcgcccgagg aggccatctc cgacatgagc
     2581 atgggcagtt cgttcgagca gatcgacttc ttccgggcag gcgtgctcgg cggcgtcagc
     2641 gagtgcaaca accgcatcgt caacagctga ccgcaccgcc ctcgtcgggc tagacccccg
     2701 cgacgaagtc gcggatcagg gcggccagtt cgaccgggcg atcgccttgc acactatggc
     2761 ccgcgccgtc gaccggctcg acccggatcg tcggctgacg acgccggcat tccgccacgt
     2821 cgtcgtcgtc gaccacgggc gacagcatgc cgcgcacgag catgatcgga ctctggatcc
     2881 tctccaacgc gccccacagt tgggccatca ccgcggtctg ctcggcaaga ctgcgctcgg
     2941 catcggcttc actcccggca tcgccgggtt cagccacgcg actctggcgg cgacggtcgt
     3001 agttccactg ccaggagcca tcggcgttgc ggtgcgcgtt gtgcagaatg ccgcggcgca
     3061 gcgacgactc gctccgggtg gggttgtgct ccatggtgcg ggcgagcagg tcggtgaagc
     3121 tggcgaagct ctgtggcccg ttgacgaagt cgtggatcgc cttggccttc tgcgggttga
     3181 cgcccggggt gacgtcgacc accaccagtt ggcgaaccag ttcggggtgg cgcgaggcca
     3241 gggcgatcga ggtcatgccg cccatcgaca tgcccaccac tgcggccgca tgcggcgcca
     3301 gcgcggtgat ggcgacggcc acatcgtcgg ccatgttgtc cgagttgtat ccgccgtctt
     3361 cccgccagcc cgagtggccg tggccgggca ggtcgatcgc gacggctggc acgccgagcg
     3421 ccagcaacac ggtgtcccac gtgtgcgcgt tttgggcacc gccgtgcagg aacaccaatc
     3481 gtggcggtcc ctcgccccac accagcgcgc tgagcgagcg tccgtccggc aactccacgt
     3541 gggtccggtg caccgggggc agttcgggca ccgccaggcc gaactcggcg atgttctcgt
     3601 ggaacaaccc gaactcgtcg tactcgatca gatcatcccg cggatcggcc ccatcgctca
     3661 ttcccccatc ctgacacccc ggcgcgcccg ccgacccagc aacagcgccg gacgacgatc
     3721 agcgcaagat cggttctggt tccacgtcag gtggagcggg tgtgatcgtc agccccgcgt
     3781 gctgactgct acggcgacgg cggggccgcg gtcgtcggag cagccgtcgt ggtcggtgcc
     3841 gcggtcgttg gcgcaaccgt cgtgggtgcc gccgtcgtgg tcggggcagc ggtcgtggcc
     3901 ggcgcggccg tcgtcgtcgg agccgacgtg gtgacgggtg gcttcgtggt gggagtggct
     3961 gcggcggtcg tggttgtctg cgacttggcc gaggtagcgg tcaccgccgc ggcagcggtg
     4021 gtcgtggtgc tcttctgctc ggccctccac tcctcgtcga ccttgtcgaa gacgggcagg
     4081 ctctcgttct tggaatactg ctccggctgg cggccgtcct gtccccagac atagaccaag
     4141 tccttgaggt gaacgtagtc ctggaagtac ttggagatgt tcatgtgcat ccgaatgcac
     4201 ccgtgcgaag ctggctccaa cgggacttcc ttcgctccgt gcagggcgat gccgtagttg
     4261 aagtagatcg ggtcgtacat tccaccgagt gcactcaccc gccgaccttg cacagcacgc
     4321 gtgaacttga atactccacc aggcgtgaac gagataccgc accgcttctc ttcgcgaggc
     4381 tcggcaagag cgacaccctt ctcctcgttg atctgcacca tctcgcacca ccagggatcg
     4441 cgccgggtca ttgcttcgcc gctggagatg tggatgatca acgtcggctt gttgtcggtg
     4501 aagacgatcg tgacctgctc gggtaggtac acctcgacgt gggtgccgct gccggggcga
     4561 cgcggcagga tcgtgatcgt gtcctgcata ccctgccaca tctcgggtgt gaccttgccg
     4621 gtcgcgtcag cacgtgggat ctgaaggacg tacttttcgt aggcccatac ggcttgcttg
     4681 gtctgctcac cgaacgctcc gtcagcgaca cctggagcga agcccaactc ggtgagacgt
     4741 gtctggatca acttgacgtc gtcaccgaac acaccctggc ggagtgtgcg tgagatggcg
     4801 gtcttgtttg ccgcgggaac tgtcgtggtg atcacgccgt cttcgacgct gtcgggcacc
     4861 gtgagnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gttgtcgtcg ccggtcgacg
     4921 ggttggtctg gtcgatcacc gcgggggtcg acgtgtcgcc cgccgccttg gcgtccttgg
     4981 gtgagtcgcc gttgagcgtg cctgccagca ccacgatcag cagcacaccg atcgccgcga
     5041 ccgcgggaat ccaccgccct gccgcgggcc cgcgcgactt cgcaccaccc gccgcggacc
     5101 gcggcttggc gttggcgacg cgacgctgag cgctcccgcc cgacgcggca cgcccggagt
     5161 tggacacatc ccgacgttca gacgctgacc gaccgagttc gccggacgtc tctgcgctgc
     5221 gccactttac ccggcgctgg cttgcatcta gcattc
//
LOCUS       DAORNF010000140         5232 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_11720_length_5232_cov_1.633958, whole genome shotgun sequence.
ACCESSION   DAORNF010000140 DAORNF010000000
VERSION     DAORNF010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 5232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5232
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11720_length_5232_cov_1.633958"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>384)
                     /locus_tag="PLV68_06985"
     CDS             complement(<1..>384)
                     /locus_tag="PLV68_06985"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRE01028.1"
                     /translation="VEQADSILPLYDAALVRPVAARLDALGVTTMVSARAVGYDAERQ
                     RLSVRLPASSGGDTGGAEAVVEVGAERVLVCVGRRPATEGWGLERLGLTRRGAAVVID
                     DRCRTSMAGVYAIGDLTGEPMLAHRA"
     assembly_gap    385..394
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<395..>761)
                     /locus_tag="PLV68_06990"
     CDS             complement(<395..>761)
                     /locus_tag="PLV68_06990"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRE01029.1"
                     /translation="ITASRPAFDLSTAMGWKDSVVARLRGGVETLLRSGGVARLHGQA
                     SIVDGKTVHVATDEGAVRVRAAHLVLATGSVPVELADLPFGGSIWSSTDLLAATDLPS
                     SLAVVGAGYIGLELGIAMA"
     assembly_gap    762..775
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<776..1065)
                     /locus_tag="PLV68_06995"
     CDS             complement(<776..1065)
                     /locus_tag="PLV68_06995"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRE01030.1"
                     /translation="MPELPTTGDVMGDVTGEAAVAVAGEAGSESVSVERDLHCDVVVV
                     GAGPGGYIAALRCAQRGLDTVVIDRAEWGGTCLNVGCIPSKAMIHVAERFAAV"
     gene            complement(1058..>1448)
                     /locus_tag="PLV68_07000"
     CDS             complement(1058..>1448)
                     /locus_tag="PLV68_07000"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HRE01031.1"
                     /translation="VLRQAHTLDLWSAAGEIARLGAAAREGRALRDELRGSSITVTSL
                     GALGGIMSTPVLNHPEVAIVGVNKLAIRPVWSDGQFVPRPMMNLSSSFDHRIVDGWDA
                     ATFVQRIKRLLEQPALLFIDHHGAGDA"
     assembly_gap    1449..1507
                     /estimated_length=59
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1508..2431)
                     /locus_tag="PLV68_07005"
     CDS             complement(<1508..2431)
                     /locus_tag="PLV68_07005"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HRE01032.1"
                     /translation="MGEYVVRLPDVGEGVAEAELVEWFVSVGDAVAADDHLADVLTDK
                     ATVEVSAPVAGRIIALIGEPGDRLAVGSGIVRLETDGPVPPRSRPAEVDPPAPVGHTV
                     AEPGDSETMSRAEPAPKPVSATAPPQPVVVPGERSGERSGERSDRPPHQWSSPRPGRR
                     PTAAPAVRRRAVELGIDLRVVTGSGPAGRITHADLDSFVAGGPALAGRARHHAEPQFS
                     DSTVKLVGLRRQIAEQMMRAVHDAATITYVEEVDMGALESLRASLNEAPPAEHRPRLT
                     ILPFLVRAIAIAVRDQPQLNATYNSERGELTL"
     gene            complement(2463..3470)
                     /locus_tag="PLV68_07010"
     CDS             complement(2463..3470)
                     /locus_tag="PLV68_07010"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HRE01033.1"
                     /translation="MTMIEAIRDAHHVAMQRDDTVVVFGEDVGYFGGVFRCTQGLQRD
                     FGRHRCFDAPISELGIVGTAIGMAAYGLRPVVEVQFADYVYPGYDQIVSEAARIRYRS
                     AGDFTVPLTIRMPSGGGIFGGQTHSQSPEALFTHVAGLKTVIPSNPYDAKGLLIASIE
                     DDDPVVFMEPKRLYNGPFDGHHERPIVPWSKHPLSEVPAGHYTVPLGQARVHRSGSAL
                     TVLTYGTMVYVALAAAQETGIDAEIIDLRSLVPLDLDTISESVAKTGRCVVAHEATLT
                     SGFGAELCAVLQEQLLYSLEAPIKRVAGWDTPYPHAHEWAYFPGPQRLGQAMRETVEA
                     V"
     gene            complement(3481..>3763)
                     /locus_tag="PLV68_07015"
     CDS             complement(3481..>3763)
                     /locus_tag="PLV68_07015"
                     /inference="COORDINATES: protein motif:HMM:NF012880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRE01034.1"
                     /translation="PSAYRPADEVQSWPLGDPIERLVRHLIRTGVWTELDHQHARAEA
                     AAEVDAAFARAEAHGVLGDEHATSPATMFEDVYADVPPALVRQRQQAGY"
     assembly_gap    3764..3839
                     /estimated_length=76
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3840..>4240)
                     /locus_tag="PLV68_07020"
     CDS             complement(<3840..>4240)
                     /locus_tag="PLV68_07020"
                     /inference="COORDINATES: protein motif:HMM:NF012880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRE01035.1"
                     /translation="DPLKGRQLPTLYSSREFGFFTISGNLGTQFIQGVGWAMASALRG
                     DTKIASSWIGDGSTAESDFHAAMVFASTYQPPVVLNIVNNQWAISTYQGFARGGSATF
                     AARGHGFGIPALRADGNDYLDAYNNVVSVGH"
     assembly_gap    4241..4324
                     /estimated_length=84
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4325..>4678)
                     /locus_tag="PLV68_07025"
     CDS             complement(<4325..>4678)
                     /locus_tag="PLV68_07025"
                     /inference="COORDINATES: protein motif:HMM:NF012880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRE01036.1"
                     /translation="IPEPLARPGESPRFAELALSPAGAVRRPEVDAGEDSLLDLASSL
                     VRVLDDDGEAVGPWSGRLSADDLVAGAHDMLRTRAFDAKMLRAQRQGKTSFYVQCLGE
                     EAISCGQARELSRGDM"
     assembly_gap    4679..4733
                     /estimated_length=55
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4814..>5232
                     /locus_tag="PLV68_07030"
     CDS             4814..>5232
                     /locus_tag="PLV68_07030"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRE01037.1"
                     /translation="MLRESAKAVPDKTLIHFMGQEFSYTQVDEMSGRVAASLEGLGLQ
                     RGDKVAVQLPNVPHFLFAYFGILKAGAVVVPLNVLLKRGEIAYHLADARAKAFFCFEG
                     TSELPMGTEGWAAFNEVGSCERFFLLTADPQAASPIVG"
BASE COUNT          789 a         1732 c         1660 g          753 t
ORIGIN      
        1 gcgcggtggg ccagcatcgg ctcgccggtc agatcaccga ttgcgtagac cccggccatc
       61 gacgtgcggc aacgatcgtc gatgactacc gctgcgccac gacgggtcag gccgagccgt
      121 tccagtcccc atccttcggt ggccggacgt cgtccgacgc agaccagaac ccgctccgct
      181 ccgacctcga cgaccgcttc tgctcccccg gtgtcgccgc cactgctcgc gggcagccga
      241 accgacaacc gctgacgctc ggcgtcataa ccaacggcgc gggccgacac catcgtcgtc
      301 accccgagcg cgtccaaccg cgccgcgacg ggtcgaacga gcgccgcgtc gtagagcggc
      361 aggatcgagt cggcctgttc gaccnnnnnn nnnnttggcc atcgcgatgc cgagttccag
      421 gccgatgtac ccggcaccga ccaccgccaa cgacgacggc agatcggtgg ccgcaagcag
      481 gtcggtggac gaccagatcg acccgccgaa gggcaggtcg gccaactcga cgggaaccga
      541 tcccgtggcc aacacgagat gggcggcacg aacccgtacg gccccctcgt cggtcgcgac
      601 gtgcacggtc ttgccatcga cgatgctggc ctggccgtgc aggcgggcga caccgcccga
      661 gcgcagcagt gtctccaccc cgccgcgcag gcgggccacc accgagtctt tccagcccat
      721 cgccgtgctc aggtcgaacg cggggcgcga cgcggtgatg cnnnnnnnnn nnnnnacagc
      781 ggcgaatcgc tcggcgacgt ggatcatcgc cttggacggg atgcagccga cgttgaggca
      841 ggtgccgccc cattcggcgc gatcgatcac caccgtgtcg aggccccgct gggcgcaccg
      901 cagcgcggcg atgtagccac ccggaccggc gccgacgacc acgacatcgc agtgcaggtc
      961 gcgttcgaca ctcaccgatt cagacccggc ctcgcccgcc accgcgaccg ccgcctcgcc
     1021 ggtcacgtca cccatcacat cgcccgtcgt cggtaactca ggcatcgccc gctccgtgat
     1081 gatcgatgaa cagcagggct ggttgttcga gcaggcgctt gatgcgttgc acgaacgtgg
     1141 ctgcatccca tccgtcgacg atgcggtggt cgaagctgct cgacaggttc atcatcggcc
     1201 ggggcacgaa ctgcccgtcg ctccagaccg gccggatcgc cagcttgttg acgccgacga
     1261 tcgcgacctc gggatggttg agcaccgggg tcgacatgat gccgccgagg gcaccgagcg
     1321 atgtgaccgt gatcgacgac ccgcgcaact cgtcgcgcag ggcgcgcccc tcgcgggctg
     1381 cagcaccgag tcgggcgatc tcgcccgcgg cgctccacag atcgagcgtg tgtgcctggc
     1441 gcagcaccnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnncag ggtgagttcg ccgcgttcgc tgttgtaggt ggcgttgagc tgcggttggt
     1561 cgcgcacggc gatggcgatc gcccgcacga ggaacggcag gatcgtcagc cgtgggcgat
     1621 gctctgccgg tggtgcctcg ttgaggctcg cgcgcagcga ttcgagggca cccatgtcga
     1681 cttcttcgac gtaggtgatc gtggccgcgt cgtgtaccgc gcgcatcatc tgctcggcga
     1741 tctgacggcg gagaccgacg agcttgaccg tcgaatcgga gaactggggt tctgcatggt
     1801 gccgggcacg tccggccagc gccggtcccc cggcgacgaa cgagtcgagg tcggcatgcg
     1861 tgatccgtcc ggccggtccc gatccggtca cgacgcgcag atcgataccg agctcgacag
     1921 cacggcggcg caccgccggt gcagcagtcg gccggcgccc tggtcgcggc gacgaccact
     1981 ggtgaggggg tctatccgac cgttcacccg accgttcacc cgaccgttca cccggtacga
     2041 cgacgggttg cggcggcgca gtcgccgaca ccggcttcgg tgcgggctcg gctcgcgaca
     2101 tcgtttccga atcgcccggt tcggcaacgg tgtgccccac gggcgcgggc ggatccacct
     2161 cggccgggcg cgaccggggc gggaccgggc cgtcggtctc tagccgcacg atgccggacc
     2221 cgaccgcgag ccggtcaccc ggttcgccga tcaacgcgat gatgcgaccc gcgaccggag
     2281 ccgatacctc gacggtggcc ttgtcggtca acacgtccgc cagatgatcg tcggccgcga
     2341 ccgcgtcacc gacagacacg aaccactcca ccagttcggc ttcggccacc ccctcaccca
     2401 cgtcgggcaa acgcaccacg tactcgccca tcacgcggcc cccatcacac ggtctcggcc
     2461 catcacacgg cctcgacggt ctcgcgcatc gcctggccga ggcgctgcgg gccagggaag
     2521 taggcccact cgtgagcgtg cgggtacggc gtgtcccagc cggcgacccg cttgatcggc
     2581 gcctccagcg agtacagcag ttgctcttgc agcaccgcac acagctcggc gccgaagccc
     2641 gaggtcagcg ttgcttcgtg ggcgaccaca caccgacctg tcttggccac cgactcgctg
     2701 atcgtgtcga ggtcgagcgg taccaggctg cgcagatcga tgatctcggc gtcgatcccc
     2761 gtctcctgcg cggccgccag ggccacgtac accatcgtgc cgtaggtcag caccgtcagg
     2821 gcgctgccgc tgcgatggac tctggcctgg ccgagcggca ccgtgtagtg gccggccggc
     2881 acctcgctga gcggatgctt cgaccacggc acgatcgggc gctcatggtg cccgtcgaag
     2941 ggcccgttgt acagccgttt gggctccatg aacacgacgg gatcgtcgtc ttcgatcgac
     3001 gcgatcagta agcccttggc gtcgtagggg ttggacggga tcaccgtctt gaggccggcg
     3061 acgtgggtga acagcgcttc ggggctctgc gagtgggtct ggccgccgaa gatcccacca
     3121 ccgctcggca tgcggatcgt gagcggcacc gtgaagtcac ccgcggagcg atagcggatg
     3181 cgggccgctt cggacacgat ctggtcgtag cccgggtaga cgtagtcggc gaactgcacc
     3241 tcgacgacgg gacgcaaccc gtacgcagcc atgccgatcg ccgtaccgac gatgcccaac
     3301 tcgctgatcg gcgcgtcgaa gcagcggtgc cgcccgaagt cacgctgcag cccttgcgtg
     3361 cagcggaaga caccaccgaa gtagccgacg tcctcgccga acacgaccac ggtgtcgtcg
     3421 cgctgcatcg ccacgtggtg cgcgtcgcgg attgcctcga tcatcgtcat gaccggcatg
     3481 tcagtacccc gcctgctggc gttggcgcac gagcgctggt ggcacgtcgg cgtacacgtc
     3541 ctcgaacatc gtcgccgggc tggtggcgtg ttcgtcaccg agcaccccgt gcgcttcggc
     3601 gcgagcgaag gccgcgtcga cctccgctgc tgcctcggcc cgagcgtgct ggtggtcgag
     3661 ctcggtccac acgcccgtac ggatcaggtg cctgacgagg cgttcgatcg ggtcacccaa
     3721 cggccagctc tgcacctcgt ccgccggtcg gtaggccgac ggannnnnnn nnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna
     3841 gtgtcccacg ctgacgacgt tgttgtaggc gtcgagatag tcgttgccgt cagcgcgcag
     3901 cgctgggatg ccaaacccgt ggccccgggc ggcgaaggtg gccgagccac cgcgagcgaa
     3961 gccctggtag gtggaaatcg cccactggtt gttgacgatg ttgagcacga cgggcggctg
     4021 gtaggtggac gcgaacacca tcgccgcgtg gaagtcggac tcggccgtcg aaccgtcgcc
     4081 gatccacgac gaggcgatct tggtgtcgcc gcgcaacgcg gatgccatcg cccatccgac
     4141 gccttggatg aactgcgtgc cgaggttgcc cgagatggtg aagaagccga actccctcga
     4201 cgagtacaac gtcgggagct ggcggccctt caacgggtcg nnnnnnnnnn nnnnnnnnnn
     4261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4321 nnnncatgtc gccgcgggac aactcgcggg cttggccgca cgagatcgct tcctcaccga
     4381 ggcactgcac gtagaacgac gtcttgccct ggcgttgggc gcgcaacatc ttggcgtcga
     4441 aggcgcgggt acggagcatg tcgtgcgcgc cggccaccag gtcgtccgcg ctgagccgac
     4501 ccgaccacgg cccgaccgcc tcgccgtcgt cgtccagtac ccgcaccagc gacgaagcca
     4561 ggtcgagcag cgagtcctcg ccggcatcga cctccgggcg gcgcacggca ccggcggggc
     4621 tgagcgcaag ctctgcaaag cgtggtgact cacccgggcg ggcgagcggc tcgggaatnn
     4681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnaatggtc
     4741 cgggccatca ggcccgggtg cacgcacggg tcggctacta ccatgaccag gcatgagctt
     4801 caacctggcg accatgctgc gcgaatcggc caaggcagta ccggacaaga ccctgatcca
     4861 cttcatgggg caggagttca gctacaccca ggtcgacgag atgtccggcc gcgtcgcggc
     4921 gtcgctggag gggctcggcc tgcagcgcgg cgacaaggtc gcggtccagt tgccgaacgt
     4981 gccccacttc ctgttcgcct acttcggcat cctcaaggcc ggtgccgtgg tcgtgccgct
     5041 caatgtcctg ctcaagcgcg gcgagatcgc ctaccacctt gccgacgccc gggcgaaggc
     5101 cttcttctgc ttcgaaggca cgagcgaact gccgatgggc acggagggct gggcggcgtt
     5161 caacgaggtc ggctcctgcg aacgcttctt cctgctgacg gcggatccgc aggcggcatc
     5221 gccgatcgtc gg
//
LOCUS       DAORNF010000141         5226 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_11740_length_5226_cov_1.849159, whole genome shotgun sequence.
ACCESSION   DAORNF010000141 DAORNF010000000
VERSION     DAORNF010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 5226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5226
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11740_length_5226_cov_1.849159"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>265
                     /locus_tag="PLV68_07035"
     CDS             <1..>265
                     /locus_tag="PLV68_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01038.1"
                     /translation="GQSFRRVATLDIGRHDPVPHDFTGRVRHSADGVVVAVGWYQNGH
                     LHNPGRLHPAYRQFRVGGQVKYDHFYRHGVLHDPNESTPAVRGY"
     assembly_gap    266..282
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <283..494
                     /locus_tag="PLV68_07040"
     CDS             <283..494
                     /locus_tag="PLV68_07040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01039.1"
                     /translation="YANGTTHYEERYRNGVREDADDGSPAVSKWRRDGTLRHVLRYRA
                     GRRLANAATDSQTGRVTGAAPSRVL"
     gene            complement(594..>2169)
                     /locus_tag="PLV68_07045"
     CDS             complement(594..>2169)
                     /locus_tag="PLV68_07045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01040.1"
                     /translation="PSASQPTPAIRVDVLGVYVPASGAVSAGRLVPLPTAVRVLDTRV
                     SGNIVSGGTTRTVPLPASIPTTASAVVVNVTVTAATGRGYWTVGPAGVLRSDTSNLNV
                     FAGETAAAQAVIGLTSLRAIEVFSQSGGHLIVDVAGWYTGATDPADTDGLFVPIPVTR
                     ALDTRDAFPLDPLSVTAVDVADAAPAQAVACNITMVAGRAPGYVTAMPAGTPRPLASH
                     VNASRTGQVVANHAIVRCSDVGVALFCQQGGHLVVDVAGWFTGTPVAVERTTIDLAQA
                     AGGLPTASVASVALGAVVAAAPGNRIAVVDPAGSVSFLSATGAVVSSASIAIPAGFSF
                     AACAVGPDDVLYLFDRDGASPAIRVFARSGSTYRQIATNATSQGHRALAATRHGIGVI
                     DEPGSLIQPYLSVGGAPSGRTLDIDQMHWSMRPNGEMTVARGEHFRVVRYTANQCLLD
                     TSNSGECITVGLGSGHDVVLTDPIDAQQRRLTWLGTDGRTAAWQGRYTYCGAIAAGLV
                     VTADGPDAQQITVVAP"
     assembly_gap    2170..2179
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2180..2625)
                     /locus_tag="PLV68_07050"
     CDS             complement(<2180..2625)
                     /locus_tag="PLV68_07050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01041.1"
                     /translation="MNAELSRRRVLALGAGAASAVWLSHSLAAHAALQEVPAGALRFV
                     PVTPTRVASTVGADGSGTGWGYVRVDGSTIRVAVAGEGSIPADARAVVANLTVSASAD
                     VDVTAYPAGTARPLASNLNIDLGGKAETDQANMITVQLDGGAMDVYV"
     gene            2917..3567
                     /locus_tag="PLV68_07055"
     CDS             2917..3567
                     /locus_tag="PLV68_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01042.1"
                     /translation="MLTVCWSVKGGSGTSVVAAAIALQSPRRSQATTLIDLAGDQPAL
                     CGLSDPSGAEARAGINDWVANSTVGPEVLDALALTVSRDLRLVPAGECRARLDHPRWA
                     QLADCLADSDGDVVVDAGLRPPRALIEVAHQSLLVTRCCYLALRNVAPHSIRPSGVVV
                     ITEPGRSITSMTVAAVAMAPVVAEIPLDPAVARRADTGMVAGRLPSALRGLARVAA"
     gene            3770..4771
                     /locus_tag="PLV68_07060"
     CDS             3770..4771
                     /locus_tag="PLV68_07060"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HRE01043.1"
                     /translation="MLPLAAPATVDALAAEATAHLDGLGPLQRLFDDPAVDEVMVNGG
                     GEVWIDRAGALTSIGRLQTGVVERIIERVLDPLGRRLDRLTPMVDARLGNGSRVCAVV
                     TPVAVDGTCLSVRRFSVRPRALTDFASESVADLLREAIERRCNTIVEGPTSSGKTSLL
                     NTLAGCVGGNERIVTLEDTAELRLAAPHVLRLEARPPSADGAPPVTLADLVRTALRLR
                     PDRLVVGEIRGAEVVDLLQALNTGHDGSLSTCHANSPADALTRLHSLVVQHHAGWPLA
                     AVRDHIRRCVDLVVHVARGADGARRVVTVAEPQVGTDEMRVLVQDGNVVAPITRGRR"
     gene            4981..>5226
                     /locus_tag="PLV68_07065"
     CDS             4981..>5226
                     /locus_tag="PLV68_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01044.1"
                     /translation="MSQRAEREASAAELAALLDAMARRCAGGESLASALADEIDGAAS
                     PALRRSLGPTVAAWHHGASLPAALARAGQGAQRDRSVS"
BASE COUNT          789 a         1839 c         1771 g          800 t
ORIGIN      
        1 ggccagtcgt tccgtcgcgt cgccaccctc gacatcggcc gccacgaccc ggtcccccac
       61 gacttcaccg gacgagtgcg gcacagcgcc gacggcgtgg tggtggcggt gggttggtac
      121 cagaacgggc atctgcacaa ccccggtcgt ctccaccccg cctaccgcca gttccgcgtc
      181 ggcggccagg tcaaatacga ccacttctac cgccacgggg tgttgcacga ccccaacgag
      241 tcgacccccg cggtgcgcgg ctactnnnnn nnnnnnnnnn nnactacgcc aacggcacca
      301 cgcactacga agagcggtac cgcaacggcg tgcgcgaaga cgcggatgac ggcagcccag
      361 cagtgtcgaa gtggcgccgc gacggcacgc tgcgacacgt gctgcgctac cgcgccggtc
      421 gccgcctcgc caacgctgcg acagacagcc agacgggccg cgtgaccggg gctgcgccca
      481 gccgggtact gtgacggcca tgaagaaggt cctcctgctg ctcgccctcc tcggtctcgc
      541 cgcgttcgcc gccaccaaac tcaaggatta gtgcgcgtca cgagcggcgc cggctacggg
      601 gcgacgacgg tgatctgctg ggcgtcggga ccatctgcgg tgacaacgag gcctgcggcg
      661 atcgcaccgc agtaggtgta tcggccctgc catgccgcgg tgcggccgtc ggtaccgagc
      721 cacgtgagac gccgctgctg cgcgtcgatc gggtccgtga gcacgacgtc gtgacctgac
      781 ccgagcccga cagtgatgca ctcacccgag ttgctggtgt cgagcaaaca ctgattggcg
      841 gtgtagcgga cgacgcggaa gtgctcgccg cgggcgacgg tcatctcgcc gttcgggcgc
      901 atcgaccagt gcatctgatc gatgtcaagc gtgcgacccg acggtgcccc gccgacggac
      961 aggtagggct ggatcagcga accaggttcg tcgatcaccc caatcccgtg tcgggtcgct
     1021 gccaacgccc ggtgcccctg gctggtggcg ttggtagcga tctgccgata cgtggacccg
     1081 gagcgggcaa agacccgaat tgccgggctc gcgccgtcgc ggtcgaacag gtacagcacg
     1141 tcgtccgggc cgacggcaca cgccgcgaaa ctgaaccccg cgggtatcgc gatcgacgca
     1201 ctcgacacga ccgcgcctgt cgcagagagg aacgacaccg accccgcagg gtcgacgacc
     1261 gcaatccggt ttccgggtgc ggcagcgacc acagccccaa gcgcaacgct ggcgacgctc
     1321 gccgtcggca gaccgccagc ggcttgggcg agatcgatcg tcgtccgctc gacagcgaca
     1381 ggtgttccgg tgaaccaacc ggccacgtcg accacgagat gcccgccctg ctggcagaac
     1441 agtgcaacgc cgacgtcgga acaacggacg atcgcgtggt tggccacgac ctgaccagta
     1501 cgagacgcgt tgacatgcga agcgagcgga cgcggagtac cggcgggcat cgccgtcacg
     1561 tagccggggg cgcggcctgc gaccatggtg atgttgcacg ccacggcctg ggcgggcgcg
     1621 gcgtcggcaa cgtcgaccgc ggtcacgctc aacgggtcga gcgggaaggc gtcgcgggtg
     1681 tcgagcgccc gggtcaccgg gatcggcacg aacagaccgt cggtgtcggc cgggtcggtc
     1741 gcacccgtgt accacccggc cacgtcgacg atcaggtggc cgccagactg cgagaagacc
     1801 tcgatggctc gcagtgacgt gagtccgatc accgcttgcg cggcagccgt ttcgcccgcg
     1861 aacacgttga ggttcgacgt gtcggagcgc aggacaccgg ctggcccgac ggtccagtat
     1921 ccgcgtccgg tcgctgcggt caccgtcacg ttcaccacga cggcgctggc cgtggtcggg
     1981 atcgacgcgg gcagcggcac ggtgcgcgtc gtgccaccgc tgacgatgtt gccgctgact
     2041 cgtgtgtcga gcacgcgcac agccgtcggc agcggcacta gccgccccgc cgacaccgcg
     2101 ccagaggccg ggacgtagac cccaagcacg tcgacgcgga tggccggggt tggttggctc
     2161 gctgacggcn nnnnnnnnna cgtacacgtc cattgctccg ccgtcgagtt gcaccgtgat
     2221 catgttcgct tggtccgtct cggccttgcc accgaggtcg atgttgaggt tcgacgccag
     2281 tggccgcgcc gtgcccgccg gataggccgt cacgtcgacg tctgccgatg ccgacacggt
     2341 gagattggcc acgacggcgc gggcatcggc cggaatcgac ccctctcccg cgacggcaac
     2401 tcggatcgtc gagccgtcga cacggacgta tccccatccg gtgcccgacc cgtcggctcc
     2461 caccgtcgag gccacccgag tcggcgtcac cggcacgaac cgcagggcac cagcaggaac
     2521 ctcttgtaac gctgcgtgtg ccgccaacga atggctcagc cacaccgccg acgcggcgcc
     2581 agccccgagc gccaacactc tccgtcgaga cagttcggcg ttcatcacat cacctcactc
     2641 ccgcagcgtc gaagacgatc ccttgtaaac ctatgcttcg gtgcgccctg cgtcaattgc
     2701 ttcttctatt tgtttgtata aatactccac ccgcctggtg aagggcgcgt tgcggcggcg
     2761 ggttggcggg ggaagaggtt ggcggcgggg gtggtgggca gcggtagcgt cgtggcacgg
     2821 cttccccccg ttgtagtgat ccgcttgtcg aactgacggc ggtgttctcc ggaggtggcc
     2881 cgtggagttc gctcccccgt tctggctggt gtcgtcatgc tgaccgtgtg ttggtcggtc
     2941 aagggcggca gcggtacatc agtggtcgcg gccgcgatcg ctctccaatc gccgcgccga
     3001 tcgcaggcga ccacgctgat cgatctggct ggcgaccaac ccgcgctgtg cggcctgtcc
     3061 gatccgtccg gcgccgaggc gcgggcgggc atcaatgact gggtcgccaa ctcgaccgtc
     3121 ggacctgagg tactcgacgc gctcgcactc accgtgtctc gcgacctgcg cctcgtcccg
     3181 gcaggcgagt gccgggcccg gctcgatcat ccgcgctggg cgcaactcgc cgactgtttg
     3241 gccgactccg acggcgacgt cgtggtcgac gccgggctgc gcccaccgcg ggcgctgatc
     3301 gaggtcgccc accagagcct gctggtgacg cggtgctgct acctcgcgct ccgcaacgtc
     3361 gccccgcaca gcatccgtcc cagcggcgtc gtggtcatca ccgagccggg ccgctcgatc
     3421 acgtcgatga cggtcgcggc ggtggcgatg gctccggtgg tggccgaaat accgctcgat
     3481 ccggccgtgg cgcggcgggc cgacaccggc atggtggccg gacggctgcc gtcggcgctg
     3541 cgcggactgg cccgagtggc ggcctgacat gcatcacgcc gcgctccaca ccaccctcgt
     3601 ccacgaagcc gcccgtcgcc tggccgatgc ccgcaccgat acgtctggtg atgccgacca
     3661 tcgggttggc gatggtggcc atggggtcgg caccgccggg cgcacggacc gccgtcagcc
     3721 aacacgtgac gacgaccatc acgatgcggc ctggcgggtc gcacggtcga tgttgccact
     3781 ggcagcgcca gcgacggtcg acgcgctggc cgcggaggcc accgcgcatc tcgacgggct
     3841 cggccccctc cagcgcctgt tcgacgatcc agccgtcgac gaagtgatgg tcaacggcgg
     3901 tggcgaagtc tggatcgatc gggccggggc actgacttcg atcggacgct tgcaaacggg
     3961 ggtggtcgag cggatcatcg agcgcgtgct cgacccgctc gggcgtcgac tcgaccgatt
     4021 gacgccgatg gtcgatgccc gtctcggcaa cggatcgcgc gtctgcgcgg tggtcacgcc
     4081 ggtcgccgtc gacggcacct gtctgtcggt gcggcgcttc tcggttcgcc cgcgagcact
     4141 cactgacttc gcgtccgagt cggtcgcgga cctgttgcgc gaggcgatcg aacgacgctg
     4201 caacaccatc gtcgaaggtc cgacctcgtc gggcaagact tcgttgctca acactctcgc
     4261 cggctgcgtc ggcggcaacg agcggatcgt cacgctcgaa gacacggccg agttacgcct
     4321 cgctgcaccg cacgtcttgc ggctggaagc ccggccgccc agcgcggacg gcgccccacc
     4381 ggtgacgctg gctgatctgg tgcgcaccgc gctgcgcttg cggcccgacc gcttggtagt
     4441 cggtgagatc cgaggtgcgg aagtggtcga tctgctccag gcgctcaaca ccggccacga
     4501 tgggtcgttg tcgacgtgcc acgccaacag tcctgccgac gcgctcacgc ggttgcactc
     4561 gctggtggtg cagcaccatg ccggttggcc gctcgccgcc gtacgcgacc acattcgtcg
     4621 ctgcgtcgat ctggtcgtcc acgtcgcccg tggagccgac ggcgcgcgtc gagtcgtcac
     4681 cgtggccgag ccgcaggtgg gcaccgacga gatgcgcgtg ctcgtgcagg acggcaacgt
     4741 cgttgccccg atcacgcggg ggcgccgatg accgccaccg ccaccgccac catcgcagca
     4801 accgtcacgt tggccgcact cgtccaagcc gggttggcca cacgcgcggc tcgaaccctc
     4861 gcgacacgcc gcctcgacag tctcacggcc gcccgccacc tcgaccgtcg tgctgccatc
     4921 cagcacccgt ccaaccgaca ctcctacatc gttcgacgtg tgcggtatgg cgtgtcacgt
     4981 gtgagccagc gggccgagcg tgaggccagc gccgcagagt tggcagcgtt gctcgacgcc
     5041 atggcgcggc gttgtgccgg cggggagtcg cttgccagcg cattggccga cgagatcgac
     5101 ggggccgcgt cgccagcact gcggcggtcg ctgggcccaa cggtcgccgc gtggcaccac
     5161 ggcgcctcgc tacctgccgc gctcgcgcgc gctggacaag gtgctcagcg tgatcgttcc
     5221 gtttcg
//
LOCUS       DAORNF010000142         5222 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_11753_length_5222_cov_1.942713, whole genome shotgun sequence.
ACCESSION   DAORNF010000142 DAORNF010000000
VERSION     DAORNF010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 5222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5222
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11753_length_5222_cov_1.942713"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..916
                     /gene="groEL"
                     /locus_tag="PLV68_07070"
     CDS             <1..916
                     /gene="groEL"
                     /locus_tag="PLV68_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019456581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HRE01045.1"
                     /translation="IIAEDIEGEALATLVVNKIRGTFNSTAVKAPGFGDRRKAMLQDL
                     ATLTGGQVISEEVGLKLESATLDLFGRAKRVIITKDNTTVVDGAGDAEDVKGRVNQIK
                     TEIENTDSDWDREKLQERLAKLAGGVAVIKVGAATEVELKEKKHRIEDAVSATRAAIE
                     EGVVAGGGTALVRSRAAVALVAESLTGDEATGARIVYESLVAPLATIADNAGLQGQVL
                     VQQVESATGSTGLNAVTGQLEDLVKAGIIDPAKVTRAALQNAASIAALVLTTECLVAD
                     KPEKKAPAMPGGAGGMGGMGGMGGMGMM"
     assembly_gap    1124..1174
                     /estimated_length=51
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1175..>2971)
                     /locus_tag="PLV68_07075"
     CDS             complement(<1175..>2971)
                     /locus_tag="PLV68_07075"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HRE01046.1"
                     /translation="PIATATPPAAASDDADGAADDTAITSSADAATDDAVVLPEMRTD
                     LASNGWAIGSELSDTGGGLLLANPHFPWEGERRLWESQLTLTTGELNVYGATLSGVPG
                     VLIGFNDAVAWTHTVSAGYRMTLYELTLTPDDPTSYVYGDDAEAMTPTDITIEVLGDD
                     GSTDEVTRTMWSSHYGPMLNLPFGWSTEKAYTYRDANLDNADILGQFFGMNQATSMDE
                     FIEAHREFNGIPWVNTMATSADGQAWYADTASAPNLSDDTIAEWQAAVDSGALAAVIL
                     DNGAILLDGSNPANEWVDDPAATRPGILPFDEQPQLLRDDFIFNANDSHWLANPDELL
                     TGYSPMTGAEEIPQSARTRMNVALLTDPELRGTDGKFSLAEIQAAIFSNRSMHADLLL
                     KQVLDVCENASTPELDNDRGPVDLTDTCDVLRDWDGTYGIDSVGAVLWREFMNRFDNT
                     DMTDAGALYAVGFDPTDPAGTPNTLNADPEPIVQALAEATFGLVEAGFGVDAVLGDLQ
                     FDGRTVDEHIPLPGGTWADGTASIVGCCSGSNSTAETGTRGTWSDIYSFSNIGYPVSF
                     GNSFMMTLQFTDDGPVAEALLNYGQPDDPADP"
     assembly_gap    2972..2984
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2985..3615)
                     /locus_tag="PLV68_07080"
     CDS             complement(<2985..3615)
                     /locus_tag="PLV68_07080"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HRE01047.1"
                     /translation="MTKRSGRAAALLVASITLAAACSSDSSSSSTTTTGAPTTDAATT
                     TTTVTSTAPSTTTATPPTDTTIDPSLYRADITRTEYGIPHIVADDWGSLGFGQGYAFA
                     QDRACTLIDQVIKVRGERAAWFGPGDDDEYVNSDFAYRHLGLHDDADERFANQPEQIA
                     EMVAGYVAGFNAELDEEGPHGWCEGEPWVQPITTTDLYAYLNDVVLFASS"
     gene            3765..4253
                     /locus_tag="PLV68_07085"
     CDS             3765..4253
                     /locus_tag="PLV68_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01048.1"
                     /translation="MSRKRSFWMHQVAEYVIAAAFVASGLQSPTPIVPSMLGVLVLLN
                     VATSQGPLSAFRLFPRRVHQVLDLALIALIAIAAVQPWWTIDGGTRAVMGLLAGALLV
                     IWWQSDFSPPQRAAAGGGTSRGPANRSSSDGVAEGAASTAGGRADTIGRAAGRLAGQW
                     TW"
     gene            4447..4911
                     /locus_tag="PLV68_07090"
     CDS             4447..4911
                     /locus_tag="PLV68_07090"
                     /EC_number="3.1.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF020622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A2"
                     /protein_id="HRE01049.1"
                     /translation="MLTALLVVPQPTLASGGGTDAVDDAYVDRMLYATSMSTFIAATK
                     GRTGPDGRSGDGWFDWTTDYCSAPLVGNTGRSFDFTNACRRHDFGYRNTHLLDKRYGK
                     GTYWNSTSRKQIDQQFLADMKAHCSARNWYDKPTCTAWAYTFYNAVRIAGGP"
     gene            complement(4878..>5222)
                     /locus_tag="PLV68_07095"
     CDS             complement(4878..>5222)
                     /locus_tag="PLV68_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01050.1"
                     /translation="EEVGLPGELVTVAGLLSPISTVVSRSYIHPVVATLSEPPRLVAQ
                     DSEVARIMWVPLAELAEPATYREEWWGTPPLNRQMTFFELDDETIWGATAHILRELLD
                     VVYGPPAMRTAL"
BASE COUNT          798 a         1649 c         1831 g          880 t
ORIGIN      
        1 gatcatcgcc gaagacatcg aaggcgaagc gctggccacc ctggtggtca acaagatccg
       61 tggaacgttc aacagcaccg ccgtcaaggc gcccggcttc ggcgatcgtc gcaaggcgat
      121 gctgcaagac ctcgcgaccc tcaccggtgg tcaggtcatc agcgaagagg tcggcctcaa
      181 gctcgagtcg gcgaccctcg acctgttcgg tcgggccaag cgcgtcatca tcaccaagga
      241 caacaccacg gtggtcgacg gcgcgggcga tgcggaggac gtgaagggtc gcgtcaacca
      301 gatcaagacc gagatcgaga acaccgactc cgactgggac cgtgagaagc tgcaagagcg
      361 tctggccaag ctggccggcg gtgttgccgt catcaaggtc ggcgccgcca ccgaggtgga
      421 gctcaaggag aagaagcacc gcatcgaaga cgctgtctcg gcaacccgtg ccgcgatcga
      481 agagggtgtg gtcgccggtg gcggcaccgc cctggtgcgc agccgtgcgg ctgtcgcttt
      541 ggtggccgag tcgctcaccg gcgacgaagc caccggagct cgcatcgtgt acgagagcct
      601 ggttgccccg ctggccacca ttgccgacaa cgccgggctc cagggccagg tgctggtgca
      661 gcaggtggaa tcggccaccg gcagcacggg cctcaacgcc gtcaccggcc agctcgaaga
      721 tctggtcaag gccggcatca tcgacccggc caaggtcacc cgggcggcgc tgcagaacgc
      781 ggcgtcgatc gcggccttgg tgctgaccac cgaatgcctc gtggccgaca agcccgagaa
      841 gaaggcgccc gccatgcctg gcggtgccgg tggtatggga ggcatgggcg gtatgggtgg
      901 catgggaatg atgtgagtcg cctttgcgac tcgcacggtt ccagcactcg ctgaccagca
      961 acaacgcaac aacgcaaacg gccccgggcg atctgcccgg ggccgttgtg cgtttcgact
     1021 cgaagccgag gccgggagcc tcagcggcgg ccggtgaccg agatcgtcgt cgcgctggaa
     1081 tcggtggcga tgtcgtcggc ggtgaacaac accgggcgga actnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnccgggt cggccgggtc gtcgggctgg
     1201 ccgtagttga gcagcgcttc ggccacggga ccgtcgtcgg tgaactgcaa cgtcatcatg
     1261 aagctgttgc cgaagctgac cgggtagccg atgttgctga agctgtagat gtccgaccac
     1321 gtgccgcggg tgcccgtctc ggccgtgctg ttcgacccgc tacagcagcc gacgatgctg
     1381 gccgtgccgt cggcccaggt accaccgggc agcggaatgt gctcgtcgac ggtgcggccg
     1441 tcgaactgca gatcaccgag cacggcgtcg acaccgaatc cggcctcgac caggccgaac
     1501 gtcgcttccg ccagggcctg gacgatcggt tcgggatcgg cgttgagcgt gttcggcgta
     1561 cctgccgggt cggtcgggtc gaagccgacc gcgtacagcg cgccggcgtc ggtcatgtcg
     1621 gtgttgtcga agcggttcat gaactcgcgc cacaacaccg cgccgacgct gtcgatgccg
     1681 taggtgccgt cccagtcgcg cagcacgtcg caggtgtcgg tcagatcgac cgggccgcgg
     1741 tcgttgtcga gttcgggcgt gctcgcgttc tcgcacacgt cgagcacctg cttcaacagc
     1801 aggtcggcgt gcatgctgcg gttggagaag atcgcggcct ggatctctgc cagcgagaac
     1861 ttgccgtcgg tgccgcgcag ttcggggtcg gtcagcagcg cgacgttcat gcgagtgcgt
     1921 gccgactgcg ggatctcctc ggcgccggtc atgggcgagt agccggtcag caactcgtcg
     1981 gggttggcca gccagtgcga gtcgttggcg ttgaagatga agtcgtcgcg cagcagttgt
     2041 ggctgctcgt cgaaaggcag gatgccgggc cgggtcgcgg ccgggtcgtc gacccattcg
     2101 ttggccggat tcgacccgtc gagcaggatc gccccgttgt cgagaatcac ggcggccaac
     2161 gcaccgctgt cgaccgcggc ttgccattcg gcgatcgtgt cgtccgacag gttcggcgcc
     2221 gaagccgtgt cggcgtacca ggcctggcca tcggccgacg tggccatggt gttcacccag
     2281 gggatgccgt tgaactcacg gtgggcctcg atgaactcgt ccatgctcgt cgcctggttc
     2341 atgccgaaga actggccgag gatgtcggcg ttgtcgaggt tggcgtcgcg gtaggtgtac
     2401 gccttctcgg tcgaccaccc gaagggcagg ttgagcatcg ggccgtagtg gctcgaccac
     2461 atcgtgcggg tgacctcgtc ggtgctgccg tcgtcgccga gcacctcgat ggtgatgtct
     2521 gtcggcgtca tcgcctcggc gtcgtcgccg tagacgtacg aggtcggatc gtccggcgtg
     2581 agggtcaact cgtacagggt catgcggtac ccggccgaca cggtgtgggt ccacgcgacc
     2641 gcatcgttga agccgatcag cacgccgggc acgcccgaca gcgtggcgcc gtagacgttc
     2701 agctcgccgg tggtgagggt gagttgtgac tcccacaggc gccgctcgcc ttcccacggg
     2761 aagtgcgggt tggccagcag tagaccgccg ccggtgtcgg acagctcgct gccgatcgcc
     2821 cagccgttgc tggccaggtc ggtgcgcatc tcgggcagca cgacggcgtc gtcggtggcc
     2881 gcatcggcgc tggacgtgat cgccgtgtcg tcggccgcgc cgtctgcgtc gtcgctggca
     2941 gcggctggtg gcgtcgcggt ggcgatcggg gnnnnnnnnn nnnngactcg acgcgaacag
     3001 gacgacgtcg ttgaggtacg cgtacagatc ggtggtggtg atcggctgca cccacggctc
     3061 gccttcgcac cacccgtggg ggccttcctc gtcgagttcg gcgttgaacc cggcgacgta
     3121 gccggccacc atctcggcga tctgctcggg ctggttggcg aaccgctcgt cggcgtcgtc
     3181 gtgcaggccg agatggcggt aggcgaagtc ggagttgacg tactcgtcgt cgtcgccggg
     3241 gccgaaccac gcggcccgct cgccgcgcac cttgatgacc tgatcgatca aggtgcaggc
     3301 ccggtcttgg gcgaaggcgt agccctggcc gaagccgagg ctgccccagt cgtcggcgac
     3361 gatgtgggga atgccgtact cggtacgggt gatgtccgcc cggtagagcg acgggtcgat
     3421 ggtggtgtcc gtcggcggcg tggctgtcgt cgtactcggc gcggtactgg tgaccgtggt
     3481 ggtcgtggtg gcagcgtcag tggtgggcgc gccggtggtg gtggtcgaac tcgatgacga
     3541 gtcgctgctg cacgcggccg cgagtgtgat gctcgcaacc agcaacgcgg ccgcgcggcc
     3601 agagcgcttc gtcaccgtcc gtttgctcga cgtcggcttg ttcaacatgg tgtttgtgtc
     3661 tccccctcgt gccggtcacg ttgagaggct gaccgcgccg aggacggcaa gggtagtttg
     3721 tgcgcgtgtc tccccgctcg accgagccca ctacggacca gtcggtgtca cgcaagcgtt
     3781 cgttctggat gcaccaggtc gcggagtacg tgatcgcggc cgcgttcgtg gccagcgggt
     3841 tgcagagccc gacgccgatc gtgccgtcga tgttgggggt gttggtgctg ctcaacgtag
     3901 ccaccagcca aggcccgcta tcggccttcc ggctgtttcc gcgccgggta caccaggtgc
     3961 tcgacctggc gttgatcgcg ctgatcgcga tcgccgcggt gcagccgtgg tggacgatcg
     4021 acggcggcac gcgcgcggtg atgggcctgc tcgcgggcgc gctgttggtg atctggtggc
     4081 agagcgactt ctcgccgccc caacgcgccg ccgccggggg cgggaccagt cgcggcccgg
     4141 ccaaccgctc gtccagcgac ggcgtcgccg agggtgcggc ctccaccgca ggcggccgag
     4201 ccgacaccat cggccgcgcc gcaggccgcc tcgccggcca atggacctgg tagaggtcga
     4261 tgacgtccgt gcgcaggcgc ttcagcgagg tttcctcggc gacaacgccg acgactttgg
     4321 tggttgcgcg aagccggcct gttcggttga atcgtgcgat cggcgctgtg ccggtcctgc
     4381 cctaggcagg tgaactgagg aggaatgatg cgacgtctcg tccatctcct cgtcgcagtc
     4441 acgagcatgc tgactgccct cttggtagta ccccaaccga cgctggccag tgggggtggc
     4501 acggacgctg tggacgacgc ctacgtcgac cgcatgctgt atgccacgtc gatgtccaca
     4561 ttcatcgccg cgaccaaggg ccgcaccggt cccgacggcc gatcaggcga tggctggttc
     4621 gactggacca ccgactactg cagcgcgccc ctggtcggca acacgggccg gtcgttcgac
     4681 ttcaccaacg cctgtcggcg ccacgacttc ggttaccgca acacccatct gctcgacaag
     4741 cgctacggca agggcacgta ctggaactcg acgtcgcgca agcagatcga ccagcagttc
     4801 ctggccgaca tgaaagccca ctgctctgcc cgcaactggt acgacaagcc cacgtgcacc
     4861 gcgtgggcgt acacgttcta caacgcggtg cgcatcgccg gcggtccgta gaccacgtcg
     4921 agcagttcgc gcaagatgtg cgccgtggct ccccagatcg tctcgtcgtc cagttcgaag
     4981 aacgtcatct gccgattgag gggcggggtt ccccaccact cctcgcggta ggtggcgggc
     5041 tcggccagtt cggccagggg cacccacatg atgcgcgcga cctcggagtc ctgggccacc
     5101 aggcggggag gctcgctgag cgtggcaacg acgggatgga tgtagctgcg gctcaccacg
     5161 gtcgagatcg ggctgagcaa gccggcgacg gtgacgagtt cgccggggag accaacctcc
     5221 tc
//
LOCUS       DAORNF010000143         5218 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_11769_length_5218_cov_2.174124, whole genome shotgun sequence.
ACCESSION   DAORNF010000143 DAORNF010000000
VERSION     DAORNF010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 5218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5218
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11769_length_5218_cov_2.174124"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1819
                     /locus_tag="PLV68_07100"
     CDS             <1..1819
                     /locus_tag="PLV68_07100"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HRE01051.1"
                     /translation="FPSLGMDVIFVDEAQKLDDGDRGILLQRVIAEAVRRAPDAQVFF
                     ASPMSANPSELLAGVPAGARTRSITSDAVTVTQNLYWVNQVRGHPRRWSVDLVSGGQP
                     ESAGEIDLPASPSPTSKRLPLVAVAMGGQSDSNVVYMNGAADAEKCAAQIYDALGPDA
                     DASADPDIAALRQLSSAIVHERYALDTVLKRRVAFHYGNMPLLLRNEIDRLFRIGALR
                     YLVCTSTLLEGVNLPCKNVFARGPRRGRGNPMSPADFWNLAGRAGRWGQEFQGNIVCV
                     DTANAEQWPTVPRTRVRYPLARAVDPVLRDLEPFVDYVQSGAPIAADAQTAAIEGVYS
                     FLAGRLARGHSLADVPGLPPISASDLERLQSVVGESLATIEYPMDLVERHAGISPLSM
                     QRLLTLFADAGDPGRLAVSPPESSDAVDTVVAALGRLAAVVGGNFGTPPRQYMLSLLI
                     VNWMRGWPLARLISGRIDYLARTGREPQVGKVIRECMADVEQFARFEAPKYLACYIDV
                     LRFHYERLGITEQMPPMPNVAMLLELGVSRTTELSLLSLGMSRSSAIGLSEFIVSDDL
                     TRDECIAWLDSRDLEQLNLPTLVVKDIRSALGRSRGTAQ"
     gene            complement(2117..3226)
                     /locus_tag="PLV68_07105"
     CDS             complement(2117..3226)
                     /locus_tag="PLV68_07105"
                     /inference="COORDINATES: protein motif:HMM:NF024554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HRE01052.1"
                     /translation="MTGEQRPRWRIEDVLNRTDLDRLLDELAVPATHQIRGRRWHCPL
                     PNHDDQHPSVTVHVDHRGHERWRCWSGDDSHRGDAIDLVALTQHVPKGEAVDWLARRA
                     GMTPDKPLPPIRRKPKPTAARAVPLNPIVVEYATACERILWSGGGRQVREWLHGRGFD
                     DELLSANHIGADPGRQMLRRRRGLPYGATVGAVFPALDPAGRIRYLQTRYLEPGRGPK
                     YDNPAASLGSNPRVAWTRPTHAPQTNVLVICEGIPDALTAAGAGLRSVAVLGSQAPDQ
                     SVASRIASYADKYELHAVAVVDNDDPGRAWGERLTGLLAEHGVEPDVIEPPNPGCDLN
                     AWAASEPEWTSQLLSEREPARAPVPVRPQARGLTV"
     gene            complement(3223..>4002)
                     /locus_tag="PLV68_07110"
     CDS             complement(3223..>4002)
                     /locus_tag="PLV68_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01053.1"
                     /translation="RPLDPTEDALLGWAVANLARTGHPFTLVDVTAAITDPADELIAL
                     SRRTPLELAQAATPIVFALDKLCSRTLAGMFDGPTTVSDRWANGPGLVVDLSAVYNDH
                     AALPLVMLAATSWLSTVLQRNSEQRVLQVIDEAWAAVHHGARHFQGSLKLSRTYGVST
                     ILVCHRPADLTAQSDDGTADAKIAAGLLSDIQTRVIFRQPIDQLPIARELFGLTEREA
                     AWVGQLVRGRALWRMQSRGLVAQTVLTQAELADFDTDQAMS"
     assembly_gap    4003..4021
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4022..4425)
                     /locus_tag="PLV68_07115"
     CDS             complement(<4022..4425)
                     /locus_tag="PLV68_07115"
                     /inference="COORDINATES: protein motif:HMM:NF014044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HRE01054.1"
                     /translation="MAHLASVYPFHVDESVTEAGPYLGVNVTGGMGGFYFDPFELYGT
                     GVLTNPNMIVTGDIGSGKSATVKAFLRRSLAVYGNRRFVAILDPKGEYLPFAVRHGLA
                     VIKLRPGGTDRLNPMDPRPGDDTGNVVVRQSLA"
     gene            complement(4506..>4841)
                     /locus_tag="PLV68_07120"
     CDS             complement(4506..>4841)
                     /locus_tag="PLV68_07120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01055.1"
                     /translation="ARAADREVMAREADAELKERKGFRVNARERKRLADVETRERELS
                     EGHAEFRFVGLVTVTSHDPDVLDDECADLEQAAAQALLDLRPLDARHDIGWVASLPLG
                     RTIAPSSAQ"
     assembly_gap    4842..4858
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4859..>5218)
                     /locus_tag="PLV68_07125"
     CDS             complement(<4859..>5218)
                     /locus_tag="PLV68_07125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01056.1"
                     /translation="DAALAALSDELRLFSARLDAGGLSVSAPLGPTDLSAAVRVRSDP
                     QRTAQLGTLTRSLAAASHRGALEWGPMVVEPEWAHVRVDGAIHRSYRIAGWPQLPVGA
                     EPVAVGVSDVAGQFLQGL"
BASE COUNT          779 a         1612 c         1884 g          907 t
ORIGIN      
        1 gtttccgtcg ctagggatgg atgtgatctt tgtagatgag gcccaaaagc tcgacgacgg
       61 tgaccgcgga atcctcctac aacgggtcat cgcggaggcc gttcgtcggg ccccggatgc
      121 ccaggtcttc ttcgcgagcc cgatgtcggc gaatccaagc gaactcctgg caggcgtccc
      181 ggccggggcg cgaactcgct cgatcacgag cgatgccgtg acagtgacgc aaaacctcta
      241 ctgggtgaac caggtgagag ggcatccgcg acgctggtcc gtcgacctcg tctcgggcgg
      301 ccaacctgaa agcgcgggcg agatcgatct gcccgcgagt ccttcgccga cgagcaagcg
      361 gcttcctctc gttgcggtcg cgatgggggg ccagtccgac agcaacgtcg tgtacatgaa
      421 cggtgccgcc gacgctgaaa agtgtgccgc gcagatctac gacgcgctag ggcccgatgc
      481 agatgcgagt gccgatccgg acatcgccgc gctgcgacaa ctctcctcag ccatcgtgca
      541 cgagaggtac gcgctcgaca cggtgttgaa gcggcgcgtt gcattccact acggaaacat
      601 gccgctccta ctgcgcaacg agatcgaccg cttgttccgg attggtgcgc ttcgctatct
      661 cgtatgtacc tcgactcttc tcgaaggcgt gaacttgccg tgcaagaacg tctttgctcg
      721 aggaccgagg cgcggccgcg gtaacccaat gtcgccggcc gacttctgga acctcgccgg
      781 tcgggcggga cggtggggac aggagttcca aggcaacatc gtctgtgtcg atacggcgaa
      841 tgctgaacag tggccaacgg tgccgcgtac gcgcgttcgt taccccttgg cgcgggccgt
      901 ggacccggta cttcgggatc tcgaaccgtt cgtcgactac gtgcagagcg gtgcgcccat
      961 tgcggctgac gcccagacgg ctgccatcga gggtgtgtac agcttcttgg cgggacggct
     1021 tgcacgtggg cactccctcg cagatgtccc gggtctccca ccgatctcgg caagcgacct
     1081 ggagcggctg cagtccgtag tcggggagtc gctggcgacg atcgagtatc cgatggactt
     1141 ggtcgaacgt cacgcaggaa tcagccctct gtcaatgcag cggctcctca cactcttcgc
     1201 cgacgctggg gatccaggtc ggctagcagt gtcgccgccc gagagcagcg acgcggtcga
     1261 caccgtggtg gcggcgctcg gtcgcctggc tgctgtggtg ggcggcaact ttggtacgcc
     1321 gcctcgccag tacatgctct cgctgctgat tgtgaactgg atgcgcggct ggccgctggc
     1381 gcgtctcatc tctgggcgca tcgattatct ggcgcgcacc ggtcgcgaac ctcaagtggg
     1441 aaaggtgatc cgtgagtgca tggcggatgt cgagcagttc gcgcgttttg aggcgcccaa
     1501 gtacctggcg tgctacatcg acgtgctgcg gttccactac gagcggctgg ggatcaccga
     1561 gcaaatgccg ccaatgccga acgtcgcgat gctgctcgag ctcggggtgt cgaggacgac
     1621 ggagctgtcg ctgctctccc tggggatgtc gcggtcgtcg gcgatcggcc tctccgagtt
     1681 catcgtgagc gacgacctca cgagagacga gtgcatcgct tggctggaca gtcgggatct
     1741 cgagcagctc aacctgccga ccttggtcgt caaggacatc aggagcgcac tgggtcgaag
     1801 ccgaggaacc gctcaatagc cgcttcaggc cggcgagcag gaccttgcgt tccgcggtcg
     1861 agtcggagtg actgtcgcag tttggcgcat ccccgaatac cggccgcagt caccgcgtcg
     1921 gcgcgccgac ttctcgaggc tgggctccga gagtggcgat gtggccgtga acggacggcg
     1981 ctgttgagcg ggaaatccgg ctggccgccc gcacctctca gcccggtacg gcaacatctt
     2041 cgtagcctgt gcatccaggg cagtctcggg cccacctggg gcgttcgcgt tgcggcggcc
     2101 tgtaaagcat cgatagtcag accgtcaagc cccgcgcttg gggccggacc gggacaggcg
     2161 cccgagccgg ctctctctcg ctcagcagtt ggctcgtcca ttcgggctcg ctcgctgccc
     2221 aagcgttcag gtcgcagccg gggttcggcg gttcgatgac gtccggttcg acgccgtgct
     2281 ccgcgaggag tccggtgagg cgctcacccc acgcacgacc tggatcatcg ttgtcgacga
     2341 cggcgactgc atggagttcg tacttgtcgg cgtagctcgc gatacgcgag gcgacgcttt
     2401 ggtcgggcgc ctgggagccg agaaccgcta ccgatcgcag cccggcgcca gccgcggtga
     2461 gagcgtccgg gatgccttcg cagatcacga gcacgttcgt ctgcggtgcg tgcgtcggtc
     2521 tcgtccaggc gactctcgga ttggagccga gactggctgc cgggttgtcg tacttcggcc
     2581 cacgcccggg ttcgaggtag cgggtctgga ggtagcggat gcgacctgcc gggtcgagcg
     2641 ctgggaacac ggctccgact gttgctccgt aggggaggcc gcgtcggcgg cgcagcatct
     2701 gtcgtccggg gtcggcgccg atgtggttgg cgctcaggag ttcgtcgtcg aagccgcgtc
     2761 cgtggagcca ctcgcgtact tgccggcctc ctccggacca caggattcgt tcgcatgcgg
     2821 tcgcgtactc gacgacgatc gggttgagtg gcaccgcccg cgctgcggtc ggctttggct
     2881 tgcgtcggat cgggggcagc ggcttgtcgg gggtcatccc ggcgcggcgg gcgagccagt
     2941 cgactgcttc gcctttgggg acgtgttggg tgagggcgac gaggtcgatg gcgtcgccgc
     3001 ggtgggagtc gtcgccggac cagcaacgcc atcgttcgtg gccgcggtgg tcgacgtgga
     3061 cggtgacgga tgggtgttgg tcgtcgtggt tggggagtgg gcagtgccag cggcggccgc
     3121 ggatctggtg ggtggcgggt acggcgagtt cgtcgaggag tcggtcgagg tcggtgcggt
     3181 tgagcacgtc ctcgatccgc catcgcggcc gctgctcgcc ggtcatgaca tggcttggtc
     3241 cgtgtcgaag tcggccagct cggcctgggt gagcacggtc tgggcgacca ggccacggct
     3301 ctgcatgcgc cacagcgccc ggcccctcac cagttggccg acccatgcgg cctcgcgttc
     3361 ggtcaggccg aacagttcgc gggcgatcgg gagctgatcg atcggctgtc ggaagatcac
     3421 tcgcgtctgg atgtccgaca acaggccggc ggcgatcttg gcgtccgctg tgccgtcatc
     3481 ggattgagcg gtgagatcgg ctgggcggtg gcagacgagg atggtcgaca ccccataggt
     3541 gcgggacagc ttgaggcttc cctggaagtg acgagccccg tgatggaccg cagcccacgc
     3601 ctcgtcgatg acttgcagca cccgttgttc cgagttgcgt tgcagcacgg tcgacagcca
     3661 tgacgtcgcg gcgagcatga cgagagggag tgctgcgtgg tcgttgtaga cggcggacag
     3721 atcgaccacg aggccgggcc cgttggccca ccggtcgctg acggtggtgg gtccgtcgaa
     3781 catgccggcg agcgtccggg agcagagctt gtcgagtgcg aacacgatcg gcgtggctgc
     3841 ctgggcgagc tccagcgggg tgcgtcgtga cagcgcgatc agctcgtctg cggggtcggt
     3901 gatcgcggcg gtgacgtcca cgagcgtgaa cgggtgaccg gtgcgggcga ggttggcgac
     3961 ggcccagccg agcagcgcgt cctctgtcgg gtcgagcggt cgnnnnnnnn nnnnnnnnnn
     4021 ngcgagactc tggcggacga cgacgttgcc ggtgtcgtcg ccggggcgcg ggtccatcgg
     4081 gttgaggcgg tcggtgccgc ccgggcggag cttgataacg gccaggccgt ggcggacggc
     4141 gaacgggagg tactcgcctt tcgggtcgag gatcgcgacg aaccgccggt tgccgtagac
     4201 ggcgagggat cggcgcagga acgctttgac ggttgccgac ttgcccgagc cgatgtctcc
     4261 cgtgacgatc atgttcgggt tggtgaggac accggtgccg tagagctcga acgggtcgaa
     4321 gtagaagccg cccatcccgc cggtgacgtt gacgccgagg tacggacccg cctcggtgac
     4381 cgactcgtcg acgtggaacg ggtagacgga cgcgaggtgt gccatcgtcg cccgatgccg
     4441 ctcgacgcgc agtcccgctc cgcctcgcag cagcggcggc cgctcaccca ctgatgtggg
     4501 ggcggtcatt gcgcgctcga tggtgcgatg gtgcggccga gtgggagcga ggcaacccaa
     4561 ccgatgtcgt gacgggcgtc gagcgggcgc aggtcgagca gtgcttgcgc ggcggcctgt
     4621 tcgaggtcgg cgcattcgtc gtcgagtacg tcggggtcgt gggatgtgac ggtgacgagc
     4681 ccgacgaacc gaaactcggc gtgaccttcg gacagctcgc gctcgcgggt ctcgacgtcc
     4741 gcgagtcgct tgcgttcgcg ggcgttgacc cgaaaccctt tgcgttcctt cagttccgcg
     4801 tcagcctcac gggccatcac ttcgcggtcc gcagcacgag cnnnnnnnnn nnnnnnnnca
     4861 ggccctgcaa gaactgcccc gcaacatcgg atacgccgac cgcgacgggc tccgcgccga
     4921 ccgggagttg cggccacccg gcgatccgat aggaccggtg gatcgccccg tcgacgcgga
     4981 cgtgggccca ttccggctcg accaccatcg gcccccactc gagcgccccg cggtgcgacg
     5041 cggccgcaag cgaccgggtg agggtgccga gctgggcggt acgttgaggg tccgaccgca
     5101 cgcggaccgc ggccgacagg tcggtcgggc cgagcggtgc cgaaacggac agcccgccgg
     5161 cgtcgagccg cgcggagaac agacgcagct cgtcgctgag cgcggcgagt gcggcgtc
//
LOCUS       DAORNF010000144         5218 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_11770_length_5218_cov_1.872555, whole genome shotgun sequence.
ACCESSION   DAORNF010000144 DAORNF010000000
VERSION     DAORNF010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 5218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5218
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11770_length_5218_cov_1.872555"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>317
                     /locus_tag="PLV68_07130"
     CDS             <1..>317
                     /locus_tag="PLV68_07130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01057.1"
                     /translation="VPDTGDDHTVALSLDALATQQAGDETVAAADDTVVVDDTIADDT
                     TVAEAATVAQDPEAESTADATEAEPVAVAEAPDAADAGEPGDDGTAVAVAETVDTREA
                     VDT"
     assembly_gap    318..382
                     /estimated_length=65
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <383..1189
                     /locus_tag="PLV68_07135"
     CDS             <383..1189
                     /locus_tag="PLV68_07135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01058.1"
                     /translation="VALFGADPGESGEAAGVPESLGRDAASTEPAPSADTVDDLFARL
                     RAARADAVVRKAQAPTAETSPAQPAASTTTRADRAAKRQAETAQPAPAEEASASAAGT
                     DRTDLTVDGDADEQQADLVPIIVAAARKLKRVLADEQNEVLDLLRRKEPVRSIDALLP
                     WELDQGDRYANAVEHELADASAAGARGDSNGRDPSLYRPAGDAVTSDIVAPLRERLAR
                     AVDVAAGDNAALATQVRAIYREWKQHRIDEYVEAIVSLAFERGALTVPTA"
     gene            1271..>2042
                     /locus_tag="PLV68_07140"
     CDS             1271..>2042
                     /locus_tag="PLV68_07140"
                     /inference="COORDINATES: protein motif:HMM:NF015644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HRE01059.1"
                     /translation="MGDRGRIALIAGFVVLVGLLVASRALSSFYVDLLWHQDLGRTDI
                     FWGVLTSKLALFGGFAVVFIVLAVLNLLIADRLVPVSLGMHSHPAVVRFHEVFGTRIR
                     LVRIVAGAVLGLIVAVPAAGHWKQWLLFRNSVSFGVDDPEFGTDIGFYVFQLPFLVFV
                     VDWLFLAVLLVLVLTVATHVLNGGIVFTPPLPRVRRATKAHFAVLLAVLALLRAAGYW
                     LERYSLTAERRGYVQGATYSVVNAQLPAVMLLMLIALAV"
     assembly_gap    2043..2089
                     /estimated_length=47
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2090..>3237
                     /locus_tag="PLV68_07145"
     CDS             <2090..>3237
                     /locus_tag="PLV68_07145"
                     /inference="COORDINATES: protein motif:HMM:NF015644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HRE01060.1"
                     /translation="AAWVVVALLGGVVYPSVVQALVVNPDQKQKEAIAIERNITATRA
                     ALGLDKVVTKTINFESLDATKVESDIEPLQDVRLLDPVEMLSRFTFDEGQLAGLRIKD
                     LDVDRYEIDGRVQQAIVAARELDLANVPNTSWQGKHLIATHGCGLVMAPASSVRADNR
                     PLYREVPLTYPQLYFSPTATDFAIVNTLTSEKPCDKTSGGTGDEGDGTVVEYSGGGGV
                     RLSSGLRKLAFAISFFDYNLWGANSVTDQSRIQWVRGVRERAEKLAPFLHFDRDPYPV
                     AIDGRVLWVIDAFTTTDQYPFAQDADRSQLDDDSGLNHVFNYVRNSVKVTVDAYTGEV
                     VFYRMDDNDPIIAAWSRAFPDMFVAASEMPLGLVDHLRYPEDLFRVQT"
     assembly_gap    3238..3263
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3264..>4089
                     /locus_tag="PLV68_07150"
     CDS             <3264..>4089
                     /locus_tag="PLV68_07150"
                     /inference="COORDINATES: protein motif:HMM:NF015644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HRE01061.1"
                     /translation="YQLEASQFFERTNAWSVAQAPRATARAQSDASSTSTTSTTQPVD
                     QQAADDAGSTTRRFVPYYTMFHEPGVAGQDGSFSILRPYVPYSKDDLLTDLQAFMVAS
                     SDVDTYGQLTAYVVRPTVDGPLKVANNAEADDRVRSEITLLNRSEDGSQVIFGDLQLV
                     PVSNGLLYVRPLYVKVDRQTSYRRVIVSYNANAVIADSIGDALADLFPGFDLDIGDRA
                     GRPTDGSSGTGNGTGTGSGSGTAQTAAELLADAQRLFDEADAALATSPPDYATFGAK"
     assembly_gap    4090..4113
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4201..4923)
                     /locus_tag="PLV68_07155"
     CDS             complement(4201..4923)
                     /locus_tag="PLV68_07155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01062.1"
                     /translation="MGLFSRKPQVDPQQVAALADEIAQLRERLDASERAKADLEVQVQ
                     QLDAAAAGMVARSQAFDNSAARLAELDVIKRQVAQLDGVQAKVAQLDALQAQVAQFDA
                     VQAKVASIDAISGRLDELADRVATSSDEARAAREQAVTLDERVTNVSTELANQLGELG
                     SELDSLAAATAANAAAAAAAAAAAADHAALKTEQTVDSDVLDKVHAAQVRLANEQARY
                     EIAFRQDLALLAEQVKRTNPGR"
     gene            complement(4979..>5218)
                     /locus_tag="PLV68_07160"
     CDS             complement(4979..>5218)
                     /locus_tag="PLV68_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRE01063.1"
                     /translation="NRSYAVLNMELSRVGASEPGPTALAMLDLTRPEMDFCALAQGMG
                     VPAERATTAEEFTAALQRALAIEGPALVEAIIPAL"
BASE COUNT          789 a         1732 c         1716 g          819 t
ORIGIN      
        1 tggtgccaga cacgggtgac gatcacaccg tcgcactgtc gctcgacgcg ctcgcgacgc
       61 agcaggcggg cgacgagact gttgctgctg ccgacgacac agtcgttgtc gacgacacga
      121 ttgcggacga cacgactgtc gccgaggcgg cgacggtcgc tcaggatccg gaggcggagt
      181 ccaccgccga cgcaaccgaa gccgagcccg tggcggtggc cgaggcgccg gatgctgccg
      241 acgcgggtga gcccggcgac gatggcacgg cggtcgcggt cgccgagacg gtcgacaccc
      301 gcgaggcggt cgacaccnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nngtggccct gttcggtgcc gatcctggcg aatctggcga
      421 ggcagcgggt gtacccgaaa gcctcgggcg cgacgcagcg tcgaccgaac cggcgcccag
      481 cgcggacacg gtcgacgacc tgttcgctcg cttgcgtgcc gcccgcgccg acgcggtggt
      541 gcgcaaggcg caggcgccta ccgccgagac cagcccggct caaccagccg ccagcaccac
      601 cacgcgcgca gatcgagcgg ccaaacgcca ggccgaaacc gcccagcccg ccccggccga
      661 agaagcgtcc gccagcgcgg ctggtaccga ccgtaccgac ctcaccgttg atggcgatgc
      721 cgacgagcag caggccgatc tggtgccgat catcgttgcc gcagcgcgca agctgaagcg
      781 ggtgctcgcc gacgagcaga acgaggtgct cgacctgttg cggcgcaaag agccggtgcg
      841 ttcgatcgac gccttgctgc cgtgggaact cgaccagggc gaccgctacg ccaacgccgt
      901 cgaacacgaa ctggccgacg cgtcggcagc cggtgctcgc ggtgacagca acggccgcga
      961 cccctcgctg taccgccccg caggcgacgc ggtgaccagc gacatcgtgg ccccgctgcg
     1021 cgagcgtctg gcccgtgctg tcgacgtggc cgcaggcgac aatgctgcgc tcgccacgca
     1081 ggtgcgcgct atttatcgcg agtggaagca gcaccgcatc gacgaatacg tcgaagcgat
     1141 cgtcagcttg gcgttcgagc gcggcgcact gacggtccca accgcctgag cgacggctac
     1201 cctcgccagg gtggccgacc ccgccgacca gcccccccag ccgatccgac ccgaccgacc
     1261 gcggcggcac ttgggtgacc gtggccgcat cgccctgatc gcgggcttcg tggtgctcgt
     1321 cggcctgctg gttgcgtcgc gggcactgtc gagcttctac gtcgacctgc tctggcatca
     1381 agacctcggc cgcacggaca tcttctgggg tgtgctcacc tccaagctgg ccctgttcgg
     1441 tgggttcgcg gtggtgttca tcgtgctcgc cgtgttgaat ctgctgatcg ccgaccggct
     1501 ggtgccggtg tcgctcggca tgcattccca ccctgcggtc gtgcgcttcc acgaggtgtt
     1561 cgggacacgc atccgcttgg tgcgcatcgt tgccggtgcc gtgctggggc tgatcgtggc
     1621 ggtgcccgcg gcgggacact ggaagcagtg gctgctgttc cgcaacagcg tgtcgttcgg
     1681 cgtcgacgat cccgagttcg gcaccgacat cgggttctac gtgttccagt tgccgttctt
     1741 ggtgttcgtg gtcgactggc tgttcttggc ggtgctgctg gtgctggtgc tgaccgtggc
     1801 gacccacgtg ttgaacgggg gcatcgtctt caccccgccg ctgccgcgcg ttcgccgggc
     1861 gaccaaggcc cacttcgcgg tgttgctggc ggtcctggcg ctgctgcgtg cggcgggcta
     1921 ctggctcgag cgctactcgc tcaccgccga acgccgcggc tacgtgcagg gggcgaccta
     1981 ctcggtggtc aatgcccagc ttcccgccgt gatgctgttg atgctgatcg ccctcgcggt
     2041 cgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng gcggcatggg
     2101 tcgtggtcgc gttgctgggc ggcgtcgtct acccgtcggt cgtgcaggcg ctggtggtca
     2161 accccgacca gaagcagaag gaagcgatcg cgatcgagcg caacatcacc gctacgcggg
     2221 ccgcgctcgg tctcgacaag gtcgtcacca agacgatcaa cttcgagtcg ctcgatgcga
     2281 ccaaggtcga gtccgacatc gagccgttgc aggacgtgcg cctgctcgat ccggtcgaga
     2341 tgctcagccg gttcacgttc gacgagggtc agctggccgg gttgcggatc aaggacctcg
     2401 acgtcgaccg gtacgagatc gacggtcggg tgcagcaggc gatcgtcgcc gcgcgcgagc
     2461 tcgacctggc caacgtgccg aacacgtcgt ggcagggcaa gcatctcatc gccacccacg
     2521 ggtgcgggct ggtcatggct ccggccagca gcgtgcgcgc cgacaaccga ccgctgtatc
     2581 gcgaggtgcc gctcacctac ccgcagctgt acttcagccc gaccgcgacc gacttcgcca
     2641 tcgtcaacac gctcaccagc gagaagccgt gcgacaagac ctccggggga acgggcgacg
     2701 aaggggacgg cacggtggtg gagtactcgg gcggtggcgg cgtgcggctc agctccggcc
     2761 tgcgcaagct ggcgttcgcg atctcgttct tcgactacaa cctgtggggc gccaactcgg
     2821 tcaccgacca gtcgcgcatc cagtgggtgc gtggcgtgcg ggagcgggcc gagaagctgg
     2881 cgccgttcct gcacttcgat cgtgacccgt acccggtcgc gatcgacggg cgcgtgctgt
     2941 gggtgatcga cgcgttcacc accaccgacc agtatccgtt cgcgcaggat gccgaccgca
     3001 gccagctcga cgacgacagc ggcctcaacc acgtcttcaa ctacgtgcgc aacagcgtca
     3061 aggtcacggt cgacgcctac accggcgagg tcgtcttcta ccgcatggac gacaacgacc
     3121 cgatcatcgc tgcctggtcg cgtgcattcc ccgacatgtt cgtggcggcc agcgagatgc
     3181 cgcttggcct ggtcgatcac ctgcgctatc ccgaggatct gttccgggtg cagaccgnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnngtaccag ctcgaggcgt cgcagttctt cgagcgcacc
     3301 aacgcctgga gcgtggccca ggcgccacgg gcgacagcac gcgctcagtc cgacgcgtcg
     3361 agcacgtcga ccacatccac gacacagcct gtcgaccagc aggcggccga cgatgcaggc
     3421 agcacgacgc gccggtttgt gccgtactac acgatgttcc acgagccggg cgtggcgggc
     3481 caggacggca gcttctccat cttgcggccg tacgtgccgt attcgaagga cgatctgctc
     3541 accgacctgc aggcgtttat ggtcgcatcc agcgatgtcg acacctacgg tcaactcacg
     3601 gcctacgtgg tgagacccac ggtcgacggc ccgctgaagg tcgccaacaa cgccgaggca
     3661 gacgaccggg tgcgcagcga gatcacgctg ctcaaccgat cggaagacgg ctcgcaggtg
     3721 atcttcggtg atctccagct ggtacccgtc agcaacggct tgctctacgt tcggccgttg
     3781 tacgtcaagg tcgatcggca gacgtcgtat cgacgggtga tcgtgtcgta caacgccaac
     3841 gcggtcatcg ccgactcgat cggcgacgcg ttggccgacc tgttccccgg tttcgatctc
     3901 gacatcggcg accgcgctgg ccgcccgacc gatgggtcga gcggcacggg taacggaacg
     3961 ggcaccggta gtggttccgg cacggcccag acagctgccg agctcttggc cgacgcgcag
     4021 cgcctgtttg acgaggccga cgcggcgctg gccacgtcgc ctcccgacta cgcgaccttc
     4081 ggcgccaagn nnnnnnnnnn nnnnnnnnnn nnngagcgtg agacaggcac aaccccatcc
     4141 acgaccgtcc ctgaccaaga cacgaccgcc tccaccgacg gctcgtaacc cgtcagggtg
     4201 tcagcggccg gggttggtgc gcttgacctg ttcggccagc agggccaggt cttggcggaa
     4261 ggcgatctcg tagcgagcct gctcgttggc gagacgcacc tgcgccgcgt gcaccttgtc
     4321 cagcacgtcg ctgtcgacgg tctgttcggt cttgagcgcg gcgtggtcgg cggccgccgc
     4381 agcagcggcc gcggccgcag cagcgttggc ggccgttgcc gcggccaacg agtcgagctc
     4441 gctgccgagc tcgccgagtt gattcgccag ttcggtcgac acgttggtga cccgctcatc
     4501 gagcgtgacc gcctgctcgc gggctgcccg ggcctcgtcg ctcgacgtcg caacgcggtc
     4561 ggccaactcg tcgagccgtc cgctgatcgc atcgatcgac gcgaccttgg cctgcaccgc
     4621 gtcgaactga gcgacctggg cctgcaacgc atccaactgg gcgaccttgg cctgcacccc
     4681 atcgagttgg gcaacctggc gcttgatgac gtccaactcg gccagccggg cggccgagtt
     4741 gtcgaaggcc tgcgatcgag ccaccatgcc tgccgcggcc gcatcgagtt gctgcacctg
     4801 tacttcgaga tcggccttgg cccgttccga tgcgtcgagc cgctcacgaa gctgggcgat
     4861 ctcgtcggcc agcgcggcga cctgctgggg atccacctgt ggcttgcgag agaacagacc
     4921 catggacgtc accatagcca gacgcccgct caccggagag cgtgatcgcg gatgatcgtc
     4981 agagggccgg aatgatcgcc tcgaccaacg cggggccttc gatggcgaga gcgcgttgga
     5041 gggcggcggt gaactcttcc gcggtggtgg cccgctcggc cggtacgccc atgccctggg
     5101 cgagggcaca gaagtccatc tccggccggg tgaggtcgag catcgccagc gcggtcgggc
     5161 ccggctcgct ggcgccgacg cgggagagct ccatgttgag caccgcgtaa ctgcggtt
//
LOCUS       DAORNF010000145         5182 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_11889_length_5182_cov_1.884533, whole genome shotgun sequence.
ACCESSION   DAORNF010000145 DAORNF010000000
VERSION     DAORNF010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 5182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5182
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11889_length_5182_cov_1.884533"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="PLV68_07165"
     CDS             complement(<1..461)
                     /locus_tag="PLV68_07165"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440627.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. The beta chain is a
                     regulatory subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HRE01064.1"
                     /translation="MTITETDRKEGRVVAIAGPVIDVEFPRGALPELNHAVEFTINVD
                     GDEIRVLAEVAQQLGNGRVRAVCLKPTDGLVRGTAVRNTGRGINVPVGDRTLGHVWNA
                     WGDVLDADNADFADVERWDIHRPAPAFDALEPSKRVFETGIKVIDLLTPYVA"
     gene            complement(496..>1045)
                     /locus_tag="PLV68_07170"
     CDS             complement(496..>1045)
                     /locus_tag="PLV68_07170"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HRE01065.1"
                     /translation="LDALLPRYVEARIYAALLNAAASEYAAKQRAMKSATDNAEELIK
                     TLARIMNRARQDSITTEIMEIVSGAEALGSGKSKRVLVIDAPFTDSASDGYLDIRPVS
                     ALAATAIGVLEPDDIEEILGIGPVYGAELRRLGVTTFAQLAAWTPADIERFEEELDAH
                     GRIEREEWVQQAQQLHTDKYGE"
     assembly_gap    1046..1081
                     /estimated_length=36
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1082..1713)
                     /locus_tag="PLV68_07175"
     CDS             complement(<1082..1713)
                     /locus_tag="PLV68_07175"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HRE01066.1"
                     /translation="MAGGQERILRGRIRSVQATKKITRAMELIAASRIVKAQQRVQAA
                     VPYSEQITEVVKDLAAAGASAASPLLSGRDEVKTTCYVVIAADRGLCGGYNANVQRAA
                     EGEIKADVLAGRDYTIVPVGRKPDGYFRFRGYNRSRSFTGFSDSPSYEDARAVGEYVV
                     QLFLDGTVDRVELVYTRFISTGSQEVVLRPLVPLDASTVAGGDGLAGTGTG"
     gene            complement(1786..>3040)
                     /gene="atpA"
                     /locus_tag="PLV68_07180"
     CDS             complement(1786..>3040)
                     /gene="atpA"
                     /locus_tag="PLV68_07180"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HRE01067.1"
                     /translation="LDGKGPLSNVQDRRMEIQAPGIMGRKPVHEPLQTGIKAVDAMTP
                     IGRGQRQLIIGDRKTGKTTIAIDAIINQRGLGVKCIYVAIGQKGSTVAQTVETLRQYG
                     AMDYTVVVSAPASDPAPFKYLAPYAGCALGQHWMETGEHALIVYDDLSKQAEAYRQVS
                     LLLRRPPGREAYPGDVFYLHSRLLERAAKLSDERGAGSLTALPVIETKAGDVSAYIPT
                     NVISITDGQVYLQDNLFKSGVRPAVDVGISVSRVGGAAQIKSMKSVSGTLKLDLAQFR
                     ELEAFATFGSELDAISKAQLERGYRLVELLKQPLNSPLPVEEQVVVIFAGTKGYLDSL
                     PVNQVRNFEAGLLEFMRSRNAGVLAGMRANPKADVPSELGDIVDAFKEQFVAGLSEAG
                     VIADPTKVAAGEIGDAESNKTLATE"
     assembly_gap    3041..3173
                     /estimated_length=133
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3174..3394)
                     /locus_tag="PLV68_07185"
     CDS             complement(<3174..3394)
                     /locus_tag="PLV68_07185"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440625.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HRE01068.1"
                     /translation="MAELTLSAGDIAAALKKNLEGFEPSLEARTVGRVTEIGDGIARV
                     SGLPDCGVNELLEFADGSVGLALNLDEES"
     assembly_gap    3548..3557
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3558..3956)
                     /gene="atpH"
                     /locus_tag="PLV68_07190"
     CDS             complement(<3558..3956)
                     /gene="atpH"
                     /locus_tag="PLV68_07190"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HRE01069.1"
                     /translation="MSDPRIDGYAKGLFEIARAEGTLDEVEDELFRFARSYESSDALR
                     SALTDELIPAGKRQAIVEDLLGGKATSTTTQLVSMVVGSGRSRDLPAIIDTLVARASS
                     AKNLEVAEVRSAVALSDDQQTRLAAALANAT"
     gene            complement(3953..4525)
                     /locus_tag="PLV68_07195"
     CDS             complement(3953..4525)
                     /locus_tag="PLV68_07195"
                     /inference="COORDINATES: protein motif:HMM:NF012645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01070.1"
                     /translation="MNLFVAFGPFVAEGGGVSVDSEGIVTHSPIWPEASELIYGTIAS
                     LLIFALLFKFAGPAIKKGLAARTAKIQGELDASADAKAAATTEAANIRQAKGDIAAER
                     ERMLADADVAAEALVADYEARLDTEIAELRARAQADIATARTREGDELRAEIARYTVA
                     AVDRAVVDSLDAATQQELIESFIQKVGATA"
     gene            complement(4522..>5182)
                     /locus_tag="PLV68_07200"
     CDS             complement(4522..>5182)
                     /locus_tag="PLV68_07200"
                     /inference="COORDINATES: protein motif:HMM:NF012645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HRE01071.1"
                     /translation="SSAAIKSPQEESGATEGDASHDESGCDPGAIATEDGCVADGDGT
                     VHLHSVEAAAPGPNPIVPEVKEVLWGAGAFIVLAVIMRYVAFPKLKKGMDARYASIRA
                     GHEQADALRATATAEVADYQAQLAAVKAEAAGRVDTARHNLEAKRSERIAAVNAEIAE
                     LRAAAAAENDASRLAVRDQIQAAVADVSASAIRIAVGKAPDAAVVDRVVADVMNTGAS
                     A"
BASE COUNT          727 a         1642 c         1748 g          886 t
ORIGIN      
        1 gcgacgtacg gggttagcag gtcgatgacc ttgatgccgg tctcgaacac gcgcttggac
       61 ggctccaacg catcgaaggc cggggccggg cggtggatgt cccagcgctc gacgtcggcg
      121 aagtcggcgt tgtcggcgtc gagcacgtcg ccccacgcgt tccacacgtg gccgagggtg
      181 cggtcaccga caggcacgtt gatgccgcgg ccggtgttgc gcacggcagt gccgcgcacg
      241 agaccgtcgg tcggcttgag gcagaccgca cgaacgcggc cgttgcccag ctgctgggcg
      301 acttcggcga ggacgcggat ctcgtcgccg tcgacgttga tcgtgaactc gaccgcgtgg
      361 ttcaactcgg gcagcgcgcc acgggggaac tcgacgtcga tcaccggacc ggcgatggcg
      421 acgacgcggc cttccttgcg gtcagtttcc gtgatggtca tggcttgtcc tgttcttctt
      481 tcactcgatc gctgatcatt cgccgtactt gtcggtgtgc aactgctgag cctgttgcac
      541 ccactcttcg cgctcgatgc gcccgtgggc gtcgagctcc tcctcgaacc gctcgatgtc
      601 ggccggggtc cacgccgcca actgggcaaa cgtggtgacg ccgaggcgac gcagctcggc
      661 gccgtacacc gggccgatgc cgaggatctc ttcgatatcg tcgggctcga gcacgccgat
      721 agccgtcgcg gccaaggccg acaccggtcg gatgtcgaga tatccgtcgc tggccgaatc
      781 ggtgaacggt gcgtcgatga cgagcacccg cttcgacttg ccgctgccga gcgcttcggc
      841 accactgacg atctccatga tctcggtggt gatgctgtcc tgacgagccc ggttcatgat
      901 ccgggccagg gtcttgatca gctcttcggc gttgtcggtc gcgctcttca tcgcccgctg
      961 cttggcggcg tactcgctgg ccgcggcgtt caacagcgcg gcgtagatgc gggcttcgac
     1021 ataacgcggc agcagggcgt cgagannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1081 ncccgtgccg gtaccggcca agccgtcgcc acccgccacc gtggacgcgt cgagcggcac
     1141 caacggacgc agcacgacct cttggctacc ggtggagatg aaccgggtgt agaccagctc
     1201 gacccgatcg accgtgccgt cgaggaacag ctgcaccacg tactcaccga ccgcgcgggc
     1261 gtcttcgtag ctggggctgt cggaaaagcc ggtgaagctg cgcgagcggt tgtagccccg
     1321 gaagcggaag tacccatcgg gcttacggcc caccggcacg atcgtgtagt cgcgaccggc
     1381 gagcacgtcg gccttgatct caccctcggc ggcgcgctgc acgttggcgt tgtagccgcc
     1441 gcacaggccg cggtcggccg cgatcaccac gtagcacgtg gtcttgacct cgtcccgccc
     1501 ggagagcagc ggcgacgcgg cggaggcacc ggcggcagcg agatccttga ccacctcggt
     1561 gatctgctcg ctgtagggaa ccgctgcctg cacgcgctgt tgggccttga cgatgcgcga
     1621 cgcggcgatg agttccatgg cgcgcgtgat cttcttcgtc gcctggacgc tgcgaatgcg
     1681 tccacgaaga atgcgctcct gacctccggc catctcaacc tgccttgctc gcgaccatga
     1741 cgctctttgg gacgctcatt gggacgttca ctgggaccgg accgctcact cggtggcgag
     1801 ggtcttgttg ctctcggcgt cgccgatctc gccagcggcg accttggtgg ggtcggcgat
     1861 gacgccggcc tcgctcaacc cggcgacgaa ctgctccttg aaggcgtcga cgatgtcgcc
     1921 caactcgctc ggcacgtcgg ccttggggtt ggcccgcatg cccgccagca ccccggcgtt
     1981 gcggctgcgc atgaactcca acagaccggc ttcgaagttg cggacctggt tgaccggcag
     2041 gctgtcgagg tagcccttgg tgccggcgaa gatgaccacg acctgctctt cgaccggcag
     2101 cgggctgttg agcggctgct tgagcagttc gaccaggcgg tagccacgtt cgagctgagc
     2161 cttggagatg gcatcgagct cggagccgaa cgttgcgaac gcttcgagct cacggaactg
     2221 ggccaggtcg agcttgagcg tgccggacac gctcttcatc gacttgatct gggccgcacc
     2281 acccacgcgc gacaccgaga tgcccacgtc gacggcggga cgcacacccg acttgaacag
     2341 gttgtcctgc aggtacacct gaccgtcggt gatcgagatc acgttggtcg ggatgtacgc
     2401 cgacacgtcg ccagccttgg tctcgatgac cggcagcgcg gtcagcgagc cggcgccgcg
     2461 ctcgtcactg agcttggcgg cccgctcgag caagcggctg tggaggtaga acacgtcgcc
     2521 ggggtaagcc tcgcggcccg gaggacggcg cagcagcagc gacacctggc ggtaggcctc
     2581 ggcctgcttg gacaagtcgt cgtacacgat cagcgcgtgc tcgccggtct ccatccagtg
     2641 ctggcccagg gcgcagcccg catagggagc caggtacttg aacggcgccg gatcggacgc
     2701 gggggccgag acgacgaccg tgtagtccat cgcgccgtac tggcgcaggg tctcgacggt
     2761 ctgggcgacc gtggagccct tctggccgat ggcgacgtag atgcacttca cgcccaggcc
     2821 acgctggttg atgatggcgt cgatcgcgat cgtggtcttg cccgtcttgc ggtcgccgat
     2881 gatcagctgg cgctggccac gcccgatcgg ggtcatcgcg tcgacggcct tgatgccggt
     2941 ctgcagcggc tcgtgcacgg gcttgcggcc catgatgccc ggcgcctgga tctccatgcg
     3001 gcggtcctgg acgttggaca gcgggccctt gccgtcgagc nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnatcgact
     3181 cttcgtcgag gttgagcgcc aggccgacgg agccatcggc gaactcgagc agctcgttga
     3241 cgccgcagtc gggcaagccg gacacacggg cgatgccgtc gccgatctcg gtgacgcggc
     3301 cgacggtacg ggcctcgagc gagggctcga aaccttcgag gttcttcttg agggctgcgg
     3361 cgatgtcgcc ggcgctgagt gtgagctctg ccatgtcgtg tccgtttctt gcggttcgtc
     3421 taccggctca gagccgggcc ttcagctgtt cgagtcgggt gcggacggaa ccgtcgatga
     3481 cggtgtcgcc cacggtggcg atcagtcccc cgagcacgga gggatcgacg accaccttga
     3541 ggttgacnnn nnnnnnnggt ggcattggcg agggcggcag cgaggcgagt ctgctggtcg
     3601 tcgctgagag cgacggccga gcggacctcg gcgacctcga ggttcttggc gctggacgcc
     3661 cgggcgacca gcgtgtcgat gatggcgggc agatcgcgcg aacggccgct gccgaccacc
     3721 atgctgacga gttgggtggt ggtggaggtg gccttgccgc cgaggaggtc ctcgacgatc
     3781 gcctggcgct tgccggccgg gatcagctcg tcggtcagcg cactgcgcag cgcgtcgctg
     3841 ctctcgtagc tgcgggcgaa gcggaacagc tcgtcttcga cctcgtcgag ggtgccctcg
     3901 gcgcgggcga tctcgaacag acccttggcg tagccgtcga tgcggggatc gctcatgctg
     3961 tggcaccaac cttttggatg aagctctcga tcagttcctg ctgggtggca gcgtcgaggc
     4021 tgtcgacgac ggcccggtcg accgcggcga cggtgtaacg ggcgatctcg gcccgtagct
     4081 cgtcgccttc gcgggtgcgg gctgtggcga tgtcggcctg agcccgggcc cgcaactcgg
     4141 cgatctcggt gtcgagacgg gcctcgtagt cggcgaccag cgcttcggcc gcaacgtcgg
     4201 cgtcggcgag catgcgctcc cgctcggcgg cgatgtcgcc cttggcttga cggatgttgg
     4261 ccgcctcggt ggtggccgca gccttggcat cggccgacgc gtcgagttca ccctggatct
     4321 tggcggtgcg cgcggccagc cccttcttga tcgccgggcc ggcgaacttg aacaacagcg
     4381 cgaagatcag cagcgaggcg atcgtgccgt aaatgagttc ggacgcctcg ggccagatcg
     4441 ggctgtgcgt gacgatgcct tcgctgtcga cgctgacgcc gcccccttcg gccacgaacg
     4501 gtccgaatgc aacgaagagg ttcatgccga tgctcctgtg ttcatcacgt cggcgacgac
     4561 gcggtcgacc actgcggcat cgggcgcctt gccgaccgcg atgcggatcg cactggcgga
     4621 cacgtcggcg accgctgctt ggatctggtc acgcaccgcg aggcggctgg cgtcgttctc
     4681 ggctgcggca gccgcccgca gttcggcgat ctcggcgttg accgccgcga tccgctcgct
     4741 gcgcttggcc tcgaggttgt gacgagcggt gtcgacccga cccgccgcct cggccttgac
     4801 cgcggcgagt tgggcctggt agtcggcgac ctcagccgtg gcggtggccc gcagcgcatc
     4861 cgcctgctcg tgaccggcgc ggatgctggc gtagcgagca tccatcccct tcttcagctt
     4921 ggggaacgcg acgtaccgca tgatgacggc gagcacgatg aacgcgccag caccccacag
     4981 cacctctttg acctcgggca cgatcgggtt gggaccgggt gccgcagcct cgacggagtg
     5041 caggtgcacg gtgccgtcac cgtcggcgac gcagccgtct tcggtcgcga tagcgccggg
     5101 atcgcagccg gactcgtcgt gcgacgcgtc gccttcggta gcaccggact cttcttgcgg
     5161 gctcttgatg gcggccgaac tg
//
LOCUS       DAORNF010000146         5165 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_11939_length_5165_cov_1.330333, whole genome shotgun sequence.
ACCESSION   DAORNF010000146 DAORNF010000000
VERSION     DAORNF010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 5165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5165
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11939_length_5165_cov_1.330333"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>234
                     /locus_tag="PLV68_07205"
     CDS             <1..>234
                     /locus_tag="PLV68_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002506219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01072.1"
                     /translation="NAGGKFGDGGYKVSGGLHGVGASVVTAMSTKVEAEVWRDGERHF
                     QTYRTKTSPAKASAGKGGPTVQLGVTDGPVQII"
     assembly_gap    235..390
                     /estimated_length=156
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <391..>1170
                     /locus_tag="PLV68_07210"
     CDS             <391..>1170
                     /locus_tag="PLV68_07210"
                     /inference="COORDINATES: protein motif:HMM:NF012430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01073.1"
                     /translation="HPGVSFVLRDRRPGADKNAEQVWFSEGGVADLVTELVTDRNLIS
                     PVLSFTGEVKGTTVAMSIVWTDGDRDTCFGYANGVANPDGGTHLDGTRRAVTEAVQKY
                     IAERSLLKEKESAPTTRDIFEGVVAVVSIMTPTPQFAGNSKSKLMTTEVAAHARSVIL
                     PQLTAWLEENPTHAKRIADAAIATMRIRTKSTADKDAAKALLARASSARPNGLPAKLR
                     DCTRKGPKGRPTELLIVEGDSAGGTTLDARDPSIQAVLPIRG"
     assembly_gap    1171..1325
                     /estimated_length=155
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1326..>1684
                     /locus_tag="PLV68_07215"
     CDS             <1326..>1684
                     /locus_tag="PLV68_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="negatively supercoils closed circular
                     double-stranded DNA; Derived by automated computational
                     analysis using gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HRE01074.1"
                     /translation="AGGDDHHIRLFGQHIRGLLTVFFYRWMPGVLESGRFFVAQPPLY
                     STIVRGERIYLADDRAKNQFLADNPNHKAPFGRLKGLGEMDAHELKTAIDPRQRRVTQ
                     IVVNEPTRLDAMARLLL"
     assembly_gap    1685..1786
                     /estimated_length=102
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1830..>2056
                     /locus_tag="PLV68_07220"
     CDS             1830..>2056
                     /locus_tag="PLV68_07220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01075.1"
                     /translation="MTAPGKRTKRSKRGTDTPADDDYVERIDQAEAADALETDHFDYS
                     VEVIEGRSIPSSADGLKTVQRRILWDMHDQG"
     assembly_gap    2057..2182
                     /estimated_length=126
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2183..>2447
                     /locus_tag="PLV68_07225"
     CDS             <2183..>2447
                     /locus_tag="PLV68_07225"
                     /inference="COORDINATES: protein motif:HMM:NF012731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HRE01076.1"
                     /translation="VLDFHGNYGSPDFGPAASRYTETRLGPLAELLLAGVDDGTVAMV
                     ENYDNTEHEPVVLPAEFPNAIINGSFGVAVGLTSHLPTHNPHEV"
     assembly_gap    2448..2457
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2458..4367
                     /locus_tag="PLV68_07230"
     CDS             <2458..4367
                     /locus_tag="PLV68_07230"
                     /inference="COORDINATES: protein motif:HMM:NF012731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HRE01077.1"
                     /translation="HVLTHPKATVAEVLELLPGPDYPGPSQVINGNELADIYARGSGT
                     IRVRGRWHVEDAARGAQVLVVDALPHNGSGLASITKFCTNIDDLVAAGELPGVIDCND
                     ESADGETRIVVRVAAGVDPDQIAAALLRNTDLQVSNTVKMHFLDHRGRVRLFNILTLI
                     RSWIDHRIVVITNRSVRRLEQIAARLDRLDGFAIVLLDIDEAIRIVRTSKTRAIALQG
                     LVAHFGVTEFQADAVLDLALGRLTEDARLEYATEAEALRVEQASLQRLIESPKLLRKQ
                     VGQELGEAAKAFVGFERQTEITTVTAALPTTVVIDAPLEVAFTTSGYVQGFKEIGKGR
                     DAKGHTVMHRVPMSTASTLLVLTDSGQLHRVLGNAIPTDKPTALQNVIQMDPAEQVLL
                     FIDAPEQMDDLILVTSAGTIKRIAGADLAGGDRKGGISIIKLDAGEKLAAVFKAPAPG
                     VPVAIVTAQGQVIRFVPDDVRPMGRSAAGVRGIKLAPKDVVVGAVPASDGKELTVFHA
                     KGSAKRVDFDGIPVQGRGGKGLRVTVVGGRHGDVAAVCNGGAAEITGRSADGELLTVN
                     RSSITLAARDAAPAKIRMFDGVIAAVVQLTPIVGGPAPDQPYIAAPVLPLDPGELAAA
                     SAATQQGELPI"
     gene            complement(4410..>4781)
                     /locus_tag="PLV68_07235"
     CDS             complement(4410..>4781)
                     /locus_tag="PLV68_07235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01078.1"
                     /translation="GNVVKGTKDGAPREIFIYQVSDHAEAFADMESQGISYTAGIPPI
                     AAAMLVADGRWDAGRMVNVEELDPEPFIELLDGMGLPTDVMEIDPASGASFQGEVGEL
                     ADEIAASTATVTAADPMIAVR"
     assembly_gap    4782..4934
                     /estimated_length=153
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4935..>5165)
                     /locus_tag="PLV68_07240"
     CDS             complement(<4935..>5165)
                     /locus_tag="PLV68_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01079.1"
                     /translation="PEINFREFVKGWTWIDREWQEFPTHAVKRTFDFPVVGEQPVYLN
                     GHDELHSLSKNIDANSIRFWMGFGPHYINVFTV"
BASE COUNT          759 a         1604 c         1434 g          666 t
ORIGIN      
        1 ccaacgcggg cggcaagttc ggcgacggcg gctacaaggt gtcgggcggc ctgcacgggg
       61 tcggcgcatc ggtggtcacc gccatgtcca ccaaggtcga agccgaggtg tggcgcgacg
      121 gcgaacggca cttccagacc tatcgcacca agacctcgcc ggccaaggca agtgcgggca
      181 agggcggccc gaccgtgcag ttgggtgtca ccgacgggcc ggtgcagatc atcgnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn catcccggcg tctcgttcgt gctgcgcgac
      421 cgcaggcccg gcgccgacaa gaacgccgag caggtgtggt tctccgaggg aggcgtggcc
      481 gacctggtca ccgagttggt caccgaccgc aacctgatct cgccggtgct gtcgttcacc
      541 ggcgaggtca agggcaccac cgtcgcgatg tcgatcgtgt ggaccgacgg tgaccgcgac
      601 acctgcttcg gttacgccaa cggcgtggcc aaccccgatg gcggcaccca cctcgacggc
      661 acccgccggg ccgtcaccga agcggtgcag aagtacatcg ccgagcgcag cctgctcaag
      721 gagaaggagt cggcaccgac cacgcgcgac atcttcgaag gtgtcgtcgc ggtggtgtcg
      781 atcatgacgc ccacgcccca gttcgcgggc aactcgaagt cgaagctgat gaccaccgag
      841 gtcgccgccc acgcgcgcag cgtcatcctg ccccagctca ccgcgtggct cgaagagaac
      901 cccacccacg ccaagcgcat cgccgacgcg gcgatcgcca ccatgcgtat ccgcaccaag
      961 tcgaccgccg acaaggacgc cgccaaggcg ctgctggccc gggcctccag cgcccgcccc
     1021 aatggcctgc cggccaagct gcgcgattgc acccgcaagg gacccaaggg ccgtccgacc
     1081 gagctgttga tcgtcgaagg cgactcggcc ggtggcacca cgctcgacgc tcgcgacccc
     1141 tcgatccaag ccgtactacc catccgcggc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnncgccg gtggcgacga tcaccacatc cggcttttcg gccagcacat ccgcggcttg
     1381 ctcaccgtgt tcttctaccg ctggatgccc ggcgtgttgg agtccggacg gttcttcgtg
     1441 gcccagccgc cgctgtacag cacgatcgtg cgcggcgagc ggatctacct cgccgacgat
     1501 cgggccaaga accagttcct cgccgacaac cccaaccaca aagcgccgtt cggccgcctc
     1561 aagggcctgg gcgagatgga cgcccacgag ctgaagaccg cgatcgaccc ccgccagcgt
     1621 cgtgtcaccc agatcgtggt caacgaaccg acgcggctcg acgcgatggc ccggttgctg
     1681 ctcgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnaggc agccgaaacg
     1801 ggcgaaccgg tcagcgccgg gagcaacgcg tgaccgcgcc gggcaagcgc accaagcgct
     1861 ccaagagggg aaccgatacc ccggccgacg acgactacgt cgagcggatc gaccaggccg
     1921 aggccgcgga cgcactcgag accgatcact tcgactacag cgtcgaggtc atcgagggcc
     1981 gatcgatccc ctcgtcggcc gacggcctca agaccgtcca gcgacgcatc ttgtgggaca
     2041 tgcacgacca ggggtannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nncgtgctcg acttccacgg caactacggc tcacccgact
     2221 tcggcccagc cgccagccgc tataccgaaa cccgtctcgg cccgctggcc gaactgctgc
     2281 tggccggggt tgacgacggc accgtggcca tggtcgagaa ctacgacaac accgagcacg
     2341 aacccgtcgt gctgccggcc gagttcccca acgcgatcat caacggctcc ttcggcgtcg
     2401 ccgtcgggct gacctcgcac ctgcccaccc acaacccgca cgaagtcnnn nnnnnnnaac
     2461 acgtgctcac ccatcccaag gccaccgtgg ccgaggtgct cgaactgctg cctggccccg
     2521 actaccccgg cccgtcgcag gtcatcaacg gcaacgagtt ggccgacatc tacgcccgcg
     2581 gcagcggcac catccgcgtg cgcggccgtt ggcacgtcga ggacgccgcg cgcggtgcac
     2641 aggtgctcgt ggtcgacgcc ctgccccaca acggttcggg cctcgcgtcg atcaccaagt
     2701 tctgcaccaa catcgacgat ctggtcgcgg cgggcgaact ccccggcgtg atcgactgca
     2761 acgacgagtc ggccgatggc gagacccgca tcgtcgtgcg tgtcgccgct ggcgtcgacc
     2821 ccgaccagat cgcagcggcc ctgttgcgca acaccgatct gcaggtatcc aacacggtca
     2881 agatgcactt cctcgaccac cgcggccggg tgcggctgtt caacatcctc accctgatcc
     2941 gctcctggat cgaccaccgc atcgtggtca tcaccaaccg cagcgtgcgt cgcctggagc
     3001 agatcgcggc ccggctcgac cggctcgacg ggttcgcgat cgtgttgctc gacatcgacg
     3061 aagcgatccg catcgtgcgc acctcgaaga cgcgcgccat cgcgctgcaa gggttggtcg
     3121 ctcacttcgg cgtcaccgag ttccaggccg acgccgtgct cgacctggcc ctcggacgcc
     3181 tcaccgagga cgctcggctg gagtacgcca ccgaggccga agcgctgcgt gtcgagcagg
     3241 cctcgttgca gcgcctgatc gagtcaccca agctgttgcg caagcaggtc ggccaggagc
     3301 tcggcgaggc cgccaaggcc ttcgtcgggt tcgaacgcca gaccgagatc accacggtca
     3361 ccgccgcgct gcccaccacc gtggtcatcg acgccccgtt ggaagtcgcg ttcaccacca
     3421 gcggctacgt gcagggtttc aaggagatcg gcaagggtcg cgacgccaag ggccacacgg
     3481 tcatgcatcg ggtgccgatg tcgaccgcgt cgacgctgtt ggtgctgacc gactcgggtc
     3541 agctgcaccg cgtgctcggc aatgccatcc ccaccgacaa gcccaccgcg ctgcagaacg
     3601 tgatccagat ggacccggcc gagcaggtgt tgctgttcat cgacgcgccc gagcagatgg
     3661 acgacctcat cctggtcacg tcggccggaa cgatcaaacg catcgccggt gccgacctgg
     3721 ccggcggcga tcgcaagggt ggcatctcca tcatcaagct cgacgcgggc gagaagctgg
     3781 cagcggtgtt caaggcaccc gcgcccgggg taccggtggc gatcgtgacc gcgcagggcc
     3841 aggtcatccg cttcgtaccc gacgacgtcc gtccaatggg ccgcagcgcg gccggggtgc
     3901 gcggcatcaa gctcgctccc aaggacgtcg tcgtcggggc ggtccccgcg tccgacggca
     3961 aggagctcac ggtgttccat gccaagggtt cggccaagcg ggtcgacttc gacggcattc
     4021 cggtgcaggg ccgtggcggc aagggcctgc gcgtcaccgt ggttggtggc cgtcacggcg
     4081 acgtcgccgc ggtgtgcaac gggggcgcgg ccgaaatcac cgggcgttcg gccgacggcg
     4141 agctgctcac ggtcaaccgc agttcgatca cgctggccgc tcgcgatgcg gcccccgcca
     4201 agattcgtat gttcgacggc gtgatcgcgg ccgtggtgca actcactccg atcgtcggcg
     4261 gccctgcccc cgaccagccc tacatcgccg ctccggtcct gccgctcgac cctggcgaac
     4321 tcgccgccgc gagcgcggcg acgcagcaag gcgagctacc gatctgagcg gttccgccga
     4381 actcgccgac agccgtcaac ggcttgcgat cagcggacgg cgatcatggg gtcggcggcc
     4441 gtcaccgtgg cagtcgacgc cgcgatctcg tcggccagct cgccgacctc gccctggaag
     4501 ctggccccac tggccgggtc gatctccatc acgtcggtgg gcaggcccat gccgtcgagc
     4561 agttcgatga acggctccgg gtcgagctct tcgacgttga ccatccgccc cgcgtcccat
     4621 cgtccgtcgg ccaccagcat cgcggccgcg attggtggga tgcccgcggt gtaactgatg
     4681 ccctgcgact ccatgtcggc gaacgcctcg gcatgatcgc tcacctgata gatgaagatc
     4741 tcccgcggtg ccccgtcctt ggtgcccttg accacgttgc cnnnnnnnnn nnnnnnnnnn
     4801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4921 nnnnnnnnnn nnnncacggt gaagacgttg atgtagtgcg gcccgaagcc catccagaag
     4981 cggatgctgt tggcgtcgat gttcttcgac agcgagtgca gttcgtcgtg gccgttgagg
     5041 tagacgggct gttcgccgac gaccgggaag tcgaacgtgc gcttgaccgc gtgggtgggg
     5101 aattcctgcc actcacggtc gatccaggtc caccccttga cgaactcgcg gaagttgatc
     5161 tcggg
//
LOCUS       DAORNF010000147         5156 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_11962_length_5156_cov_1.558518, whole genome shotgun sequence.
ACCESSION   DAORNF010000147 DAORNF010000000
VERSION     DAORNF010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 5156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5156
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11962_length_5156_cov_1.558518"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..789)
                     /locus_tag="PLV68_07245"
     CDS             complement(<1..789)
                     /locus_tag="PLV68_07245"
                     /inference="COORDINATES: protein motif:HMM:NF014789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRE01080.1"
                     /translation="MAEITAEQVRDEVTAWVQQNWDPEITLGEWWKRLAGAGYASPML
                     PESCGGKGYPRHLAVLVMNILGEQHTIAPPTGLGLMLAAPTIAAHGSPEQQAEYIPRI
                     LDGTDAWCQLFSEPGAGSDLAGLGTKADLDGDEYVITGQKVWTSGGQYANWGMLIART
                     NPDAPKHQGISYFAFEMDQPGVDVRPLREMTGRAMFNEVFIDEARVPAANLIGGLNNG
                     WAVANTTLMVERASLGSGGGGGSGAAQVTPGPIADQLGRRAGDLA"
     gene            complement(758..1111)
                     /locus_tag="PLV68_07250"
     CDS             complement(758..1111)
                     /locus_tag="PLV68_07250"
                     /inference="COORDINATES: protein motif:HMM:NF016141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HRE01081.1"
                     /translation="MGLLAWIVIGLLAGGIARWVVKDDRSGCIYTMVVGVIGALIGGA
                     LVNAAGGSGVIELNIRSVLVAAIGAIVLLLVLQALNGVSGNPRRRRSVSFASLTARDR
                     FYHRRHGRDHSRAGP"
     gene            1243..3171
                     /locus_tag="PLV68_07255"
     CDS             1243..3171
                     /locus_tag="PLV68_07255"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRE01082.1"
                     /translation="MNTPESDAVPWHLLTGLLKTRRTGLAVGVVIGVLWAASKVSVPR
                     LVRLAIDHSINGDEPLVVWAGVIAGVGVVSAIFMAWRRWLAFNESRGIETELRAQLFD
                     HILSLDVAYHDQAQTGQLMSRASSDLQQIQAFVVMIPLVLGNAFQLVAITVLLATSQP
                     LLALLALAPLPLVNVMAKRFSTRIHPAVMAVQAEQAALATVVEESVSGVRVVKGFGAE
                     AVQSAKLRARADAIRDVSFGAARIRSSFVPAIDLLPSLGLIAVLALGGHRVIDGAMTI
                     GQLVEFNSSVLLLIWPMRNIGMTLAVGQRAAVALRRVQQVLSTVPTITDPTDPVLFRL
                     LPPGEWGGDSTAGERAAGEVRFRDVVFGYDPALPVLRGVDLTIPAGSSVAVVGATGAG
                     KSTIVRLLLRFYDVGQGGIEIDGVDIRQRRLADLRRTVGVVFEDTVLFHDTVAANIAF
                     AKPDAAQEQIARAARLAGSAEFIAALPQGEETTIGERGMSLSGGQRQRIAIARAIVAD
                     PAVLVLDDATSAVDPAKEHEIREAMATVMRDRTTIVIAHRPGTIALADRVVLLDGGRI
                     AAVGTHDELLATNARYRAVLAAWAESDREPDDHADSVVIDADPSGQAARDRLHLAAED
                     ATEDLVADSPLHEPAAGA"
     gene            3183..>3999
                     /locus_tag="PLV68_07260"
     CDS             3183..>3999
                     /locus_tag="PLV68_07260"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRE01083.1"
                     /translation="MSAISEDDKLDRAATTKVARRSWQLARPFRRTIWWALGLITVWT
                     ATTLAGPVIVQYGIDHGIAASNVSALNAAVAAYVVVTVMAYVLGRLQFLAVNRAGEGF
                     LRRLRVDVFDHLQRQGMAFYDREKTGVIVSRMTADIESMAELIQFGLLQFVSAAILVV
                     MAIALLFALSWQLTLVVLIAVPVLGVASVKFQRDSNRAYLDVRDRVGANLGALQEGIS
                     SVRVIQAYAREREQSRRFRRSNDALFDSHMHSVRVSMWYFALVELCGVVAIAAI"
     assembly_gap    4000..4049
                     /estimated_length=50
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4050..>4461
                     /locus_tag="PLV68_07265"
     CDS             <4050..>4461
                     /locus_tag="PLV68_07265"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRE01084.1"
                     /translation="SSLFDPVQQLSQLYNTVQSAGASLQKLYTILDTVPDVGEAPDAV
                     DLPAAGDLVVGGAEFAYVAGRPVLHAVDLTIGAGERVALVGPTGAGKSTLAKLMARLY
                     DPTAGTICFGGVDLRQATMSSLRERIVVVPQEGFL"
     assembly_gap    4462..4524
                     /estimated_length=63
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4525..4919
                     /locus_tag="PLV68_07270"
     CDS             <4525..4919
                     /locus_tag="PLV68_07270"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRE01085.1"
                     /translation="IGALDRFLALPEGLDTEVRERGSRLSAGERQLIALARAALVDPA
                     VLVLDEATSNLDPGTEAAVEHALEALMTGRTVIVVAHRLSTVQRADRIAVIADGGLAE
                     LGSHDELIAQGGRYAALAAAWQRNHVAS"
BASE COUNT          730 a         1655 c         1796 g          862 t
ORIGIN      
        1 ggcgaggtca ccggcgcggc gaccgagctg gtcggcgatc gggccgggag tgacctgggc
       61 cgcgccgctg ccgccaccgc cgccactgcc caggctggcc cgctcgacca tcagcgtggt
      121 gttggccact gcccagccgt tgttgagccc accgatcagg ttggcggcgg ggactctggc
      181 ctcgtcgatg aacacctcgt tgaacatcgc ccgtccggtc atctcacgca gtgggcgcac
      241 gtcgacaccg ggctggtcca tctcgaaggc gaagtaggag atgccctggt gcttgggggc
      301 gtcggggttg gtgcgggcga tcagcatgcc ccagttcgcg tactgaccac cgctggtcca
      361 caccttctgg cccgtgatga cgtactcgtc gccgtcgagg tcggccttgg tgccgagccc
      421 ggccaggtcg ctgccggcac cgggttcgct gaacagctgg caccacgcgt cggtgccgtc
      481 gaggatgcgc gggatgtact cggcctgctg ctccgggctg ccgtgggcgg cgatggtcgg
      541 tgccgccaac atcagcccca gtccggtcgg cggggcgatg gtgtgctgct cgccgaggat
      601 gttcatcacc aacacggcca ggtgccgcgg gtagcccttg ccaccacacg actcgggcag
      661 catcggcgag gcgtatcccg cgccagccag tcgcttccac cactcgccga gggtgatctc
      721 ggggtcccag ttttgctgca cccacgccgt gacctcgtca cggacctgct ctgctgtgat
      781 ctcggccatg acggcgatgg tagaagcggt cccgcgccgt caacgaggcg aacgaaaccg
      841 aacggcggcg gcgagggtta cccgatacac cgttgagcgc ttgcagcacc aacagcagca
      901 cgatcgcgcc gatggccgcg accagcaccg acctgatgtt gagctcgatc accccggaac
      961 ctccggcggc gttgaccagt gcaccgccga tcagcgctcc gatcaccccc acgaccatgg
     1021 tgtagatgca cccgctgcga tcgtccttga ccacccagcg tgcgatgcct ccggcgagca
     1081 gtccgatcac gatccaggcg agcaacccca cgatcggcac tctaacgaca cccgctcggg
     1141 gacgcgcgga aacccgcttg ctgccgtaac tcgcttgctg ccgtaactcg cttgatcatg
     1201 gggcaggtcg cgggcgtatg gtcccgcaac ggaatgggac acgtgaacac gcctgaatcg
     1261 gacgccgtgc cgtggcacct gctgacaggc ttgttgaaga cgcgtcgcac cggtctggcg
     1321 gtgggagtcg tcatcggtgt gctgtgggcg gcgagcaagg tcagcgtgcc gcggctggtg
     1381 cgtctcgcga ttgatcacag catcaacggc gacgaaccgc tggtggtgtg ggccggggtg
     1441 atcgccgggg tcggcgtggt ctcggcgatc ttcatggcct ggcggcgatg gttggccttc
     1501 aacgagagcc gggggatcga gaccgaactg cgcgcccagc tgttcgacca catcctgtcg
     1561 ctcgacgtcg cctaccacga ccaggcccag accggccagt tgatgagccg ggcgtcgagc
     1621 gatctccagc agattcaagc cttcgtcgtg atgatcccgc tggtgctggg caacgcgttt
     1681 caactcgtgg cgatcaccgt gctgctggcg accagccagc cgctgctcgc cctgttggcc
     1741 ttggcgccgc tgccattggt caacgtgatg gcgaagcggt tcagcacccg gatccatcca
     1801 gcggtgatgg cggtgcaggc cgagcaggcc gcattggcga ccgtggtcga agagagcgtc
     1861 tccggggtgc gcgtggtcaa gggcttcggg gccgaggcag tgcagtcggc caagttgcgc
     1921 gcccgtgccg acgcgatccg tgacgtgtcc ttcggcgcgg ctcggattcg cagctcgttc
     1981 gtaccggcga tcgatctgtt gccgtcgctg gggctgatcg cggtgctcgc cctgggtggc
     2041 caccgggtga tcgacggggc gatgacgatc ggccaactcg tcgagttcaa ctcgagcgtg
     2101 ttgctgctga tctggccgat gcgcaacatc ggcatgaccc ttgcggtcgg ccagcgagcc
     2161 gcggtggcgc tgcggcgggt gcaacaggtg ttgagcaccg tgcccacgat caccgacccc
     2221 accgaccccg tgctgttccg actgctccca cccggcgagt ggggtggcga ttcgaccgct
     2281 ggggagaggg ccgcgggcga ggtgcggttc cgtgacgtcg tgttcggcta cgacccggcg
     2341 ttgccggtgc tgcgtggcgt cgacctgacc atccccgccg gctcgtcggt ggccgtggtg
     2401 ggcgcgaccg gcgcgggcaa gtcgacgatc gtgcggctgc tgctgcggtt ctacgacgtc
     2461 ggtcagggtg gcatcgagat cgacggggtc gacatccgcc agcgtcggct ggccgatctg
     2521 cgccgcacgg tcggtgtggt gttcgaagac acggtgctgt tccacgacac ggtcgccgcc
     2581 aacatcgctt tcgccaagcc cgatgccgcc caggagcaga tcgctcgcgc cgcccgcttg
     2641 gccggctcgg ccgagttcat cgctgccttg ccacagggtg aggagacgac gatcggtgag
     2701 cgaggcatgt cgctgagcgg tggtcagcgc cagcggatcg cgatcgcccg ggcgatcgtg
     2761 gccgacccag cggtgctggt actggacgac gcgacctccg cggtcgaccc ggccaaagag
     2821 cacgagatcc gtgaagcgat ggcgaccgtg atgcgcgatc ggaccacgat cgtgatcgct
     2881 caccggccgg gcacgatcgc gctcgccgac cgggtggtgt tgctcgacgg gggccggatt
     2941 gctgccgttg gcacccacga cgagttgctc gccaccaacg cccgctaccg ggccgtgctc
     3001 gccgcgtggg ccgagtcgga tcgagagccc gacgaccacg ccgactcggt ggtgatcgac
     3061 gccgatccgt ccggccaggc cgctcgcgac cggttgcact tggcggcgga ggacgcgacc
     3121 gaggacctgg tcgccgactc accgctgcac gaaccggcgg caggtgcctg atgggctggg
     3181 cgatgagcgc gatcagcgag gacgacaagc tcgaccgcgc cgcgaccacc aaggtggcac
     3241 gccgtagctg gcagctggca cgaccgttcc ggcgcacgat ctggtgggcg ctcgggctga
     3301 tcaccgtgtg gaccgcgacc acgctggccg ggccggtcat cgtgcagtac ggcatcgatc
     3361 acggcatcgc tgcctcgaac gtctcggcgt tgaacgccgc cgtagccgcc tacgtggtgg
     3421 tcaccgtgat ggcgtacgtg ttggggcggt tgcagttcct ggcggtcaac cgtgccggcg
     3481 agggtttcct gcgcaggctg cgggtcgacg tgttcgacca cctccaacgc cagggcatgg
     3541 ccttctacga ccgtgagaag accggtgtga tcgtgtcgcg gatgaccgcc gacatcgagt
     3601 cgatggccga gctgatccag ttcggcctgc tccagttcgt gtccgcggcg atcttggtgg
     3661 tgatggcgat cgcgctgctg ttcgccttga gttggcagct cacgctggtg gtcctgatcg
     3721 cggtgcccgt gctgggagtg gcgtcggtga agttccagcg cgactcgaac cgggcttacc
     3781 tcgacgtgcg tgatcgggtt ggcgccaacc tgggtgcgct ccaggaaggc atcagcagcg
     3841 tccgtgtcat ccaggcctac gcccgcgagc gcgagcagtc acgccggttc cggcggtcga
     3901 acgatgcgct gttcgacagc cacatgcaca gtgtgcgggt ctcgatgtgg tacttcgcct
     3961 tggtcgagct gtgcggcgtc gtcgcgatcg cggcgatcgn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt gagcagcctg ttcgacccgg tgcagcaact
     4081 gtcgcagctc tacaacacgg tgcagtcggc gggggcgtcg ctgcagaagc tgtacaccat
     4141 cctcgacacg gtgcccgatg tgggcgaggc acccgacgcg gtcgacctgc ccgccgcggg
     4201 cgatctcgtc gtcggcggtg ccgagttcgc ctacgtggcc gggcgacccg tactgcatgc
     4261 ggtcgatctc accatcggcg ccggcgagcg ggtggcgctg gtgggcccga ccggtgcggg
     4321 caagagcacc ctggccaagt tgatggcccg gctgtacgac cccaccgcag gcacgatctg
     4381 cttcggcggt gtcgacctgc gccaggcgac gatgtcgtcg ctgcgggagc ggatcgtcgt
     4441 ggtgccgcag gagggcttcc tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnncgatcg gagccctcga tcgctttttg gcccttcccg
     4561 agggtctgga caccgaggtg cgcgagcgcg gttcgcggct gtcggccgga gagcgacaac
     4621 tgatcgcgct ggcccgcgcg gcgttggtcg acccggcggt gttggtgttg gacgaagcga
     4681 cgtcgaacct cgaccccggt accgaggccg cggtcgagca cgcactcgaa gcgctgatga
     4741 ccgggcggac ggtgatcgtc gtggcgcacc gactgtcgac ggtgcagcgc gctgaccgca
     4801 tcgccgtcat cgccgacggt ggcctggccg agctcggctc acacgacgaa ctgatcgcgc
     4861 agggcggccg ctatgccgcg ctggccgcgg cgtggcagcg caaccacgtc gcgtcctgat
     4921 cgggtcgtgg gcccgcattg cggttcccac caggtgtcga cgagtgcccg tcaggctctg
     4981 tgcccgtcag gctccgcgcg tgaacgtcag caggaactcg agtgcaccgt ggtcttgggt
     5041 cgtgatgccg ccggtcgagg ggttgtcgat gccgtagtcg gcgaacacga tctgggtcga
     5101 gccgaccacg tcgacgatgt caccgttgcg ttgggcctgg acggtgatcg tgaccg
//
LOCUS       DAORNF010000148         5129 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_12039_length_5129_cov_1.884706, whole genome shotgun sequence.
ACCESSION   DAORNF010000148 DAORNF010000000
VERSION     DAORNF010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 5129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5129
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12039_length_5129_cov_1.884706"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    224..299
                     /estimated_length=76
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <300..803
                     /locus_tag="PLV68_07275"
     CDS             <300..803
                     /locus_tag="PLV68_07275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01086.1"
                     /translation="TPPARARVHRIRHIALALIAAVFASTIAVSAATPVALAATTHTV
                     TWDMGGHGANIEVQVEDGQEPDWPNFDVPIGKTFVDFYVEPTFDTSGYPFGPITQDTT
                     FYAKFVPATYTASFNMQGHGIQIAQVQDGTPAPRRPPRTTSPPWSPLRSPSTRSGRSP
                     PTPCRSP"
     gene            734..>2224
                     /locus_tag="PLV68_07280"
     CDS             734..>2224
                     /locus_tag="PLV68_07280"
                     /inference="COORDINATES: protein motif:HMM:NF024702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat protein"
                     /protein_id="HRE01087.1"
                     /translation="MVTAPITLYAKWTITTYTVSFSMNGFGVQVPAQSVHHGSAATAP
                     APPTDPDGVFVGWYTTAATTTAYDFSTPVTGDVTVFAKWSAACVNVAGGGVINGYTNG
                     FYWTADSSNCAGGATIVNGDYGPVTTGNVVLPATFTDVGNSSDGDNVYKVTAIADDAL
                     QFRSITAITLPPTLRSIGANALWYNSLTSLTLPQSVTSIGTEAFSGNQITSITWPSGP
                     VTLGDSVFSSNKLAALTLPSGLTAIPKFTFSNNVLTSVTLPEGLISVGEQAFASNKLT
                     SLTLPQTVTTLGFAAFVNNPIVSVNIPASVTSLAVNVVKHSALKSVFFEGAPPATFAV
                     GAVNTDGSFGFGTNLTGTSGPVLYFRPQFSELVYPGGFSTPQWKGYRTAYVPSAVDTV
                     SPGRYWDTRNEPTFDGRFRNTGRLAGGQSFAIEIRGRGNVAGDATGVIANLTVIDAAG
                     AGYATLYPCGGTVPTASTVNYTTGAIVANNAFVPLAANGTVCVFTSN"
     assembly_gap    2225..2278
                     /estimated_length=54
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2279..3028
                     /locus_tag="PLV68_07285"
     CDS             <2279..3028
                     /locus_tag="PLV68_07285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01088.1"
                     /translation="VGITPARYLDTRGGSDTTFDGAFSGGGVVYGTTKTVQIAGRGNV
                     AAGAKAAFINLTVVNNSTLPGFVTVYPCDAPRPTASTLNVGAYGVVANGAMVKLAADG
                     TLCLFSSTLNYLVVDVTGYVPAGSDSISPLVPSRLFDSRAGEPIVDGVGAGPRLAAGS
                     TVEVQVTGRVNIPVTATAAFLNVVVVSPDGPGFATLYPCGTLPGTSSVNYTTGTIVAN
                     NATTMLSPTGSVCIYVMRGTDVVLDVTGYIE"
     gene            3069..3581
                     /locus_tag="PLV68_07290"
                     /pseudo
     CDS             3069..3581
                     /locus_tag="PLV68_07290"
                     /inference="COORDINATES: protein motif:HMM:NF025052.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            complement(3612..4631)
                     /locus_tag="PLV68_07295"
     CDS             complement(3612..4631)
                     /locus_tag="PLV68_07295"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HRE01089.1"
                     /translation="MNEASNREPTSTVAAAAAVVGVEPEADLAGSGALLRRIRATEFD
                     PGTTRAEALVAIVEDPAQPWPRRHRAGLLLAIGGDPRIDTFDPPMIDVPATTYPMGTA
                     AADVDRVLARWANVGVERAWIDKEVPRHTVTVAAFRLGRYPVTNEEYLDFVLATGYPG
                     RPSSWGHGTFPLGAANQPVYTVAPSDADAYCAWLSERTGRHFRLPTEAEWELTATGGD
                     GREYPWGDEWNPARANTAEAGPLTTTPIGIYPEGDSPFGASDMAGNVEEYVSDTYRPY
                     PGGPTVADDLVATDGEHYRVARGGSFALAGDLARCARRHGWYRSPHYAMGFRLAETIE
                     LVPAH"
     gene            4743..>5129
                     /locus_tag="PLV68_07300"
     CDS             4743..>5129
                     /locus_tag="PLV68_07300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01090.1"
                     /translation="MHHLGLDAIVDLPPELNGGLTFHLPLEVFALEHAAASAAHIDAR
                     IEAELAVAFRDGLVDPPFYRWGIEQYRPLATTLLASDAAARCQAVQSVAPLGDGLAGA
                     AFHGLIRLGFGALRRDADEIARGLAYL"
BASE COUNT          763 a         1740 c         1683 g          813 t
ORIGIN      
        1 cgacggtgaa cgctgccgcg atcgccaagc ggcgacggtc cgtggcgtcg aggttcagcg
       61 ggtcctcctc aggcgtccag tcctccacgg cggagggaac tccatgaggc taacggcccc
      121 gccccgttcc ggtacgccac ccgtggcctg cgacgttgcg gcaccgggtt tgcaggcgtg
      181 tagcgtgccg ctcggacacg gcgccctgcg cgggctggcg gaannnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna
      301 ccccgcccgc gcgtgctcgt gtccaccgca tccgccacat cgcgctggcg ctgattgcgg
      361 cggtgttcgc cagcaccatc gccgtcagcg cggcgacgcc ggttgccttg gcggcgacga
      421 cgcacacggt cacctgggac atgggcggtc acggggcgaa tatcgaggtc caggtcgagg
      481 atgggcagga gcccgattgg ccgaacttcg acgtaccgat cggcaagacg ttcgtggact
      541 tctacgtgga accgacgttc gacacctcgg ggtatccgtt cgggccgatc acgcaggaca
      601 ccaccttcta cgccaagttc gtcccagcga cctacaccgc gtccttcaac atgcaaggac
      661 acggtatcca gatcgctcag gtccaggatg gtacaccagc gccgcgacga ccaccgcgta
      721 caacttcgcc accgtggtca ccgctccgat caccctctac gcgaagtgga cgatcaccac
      781 ctacaccgtg tcgttctcca tgaacggctt cggcgtccaa gtgcccgccc agagcgtgca
      841 ccacggttcg gccgccaccg cgcccgcccc gccgacggac cccgacggtg tgttcgtcgg
      901 ttggtacacc acggccgcga cgaccacggc gtacgacttc tccacgccgg tgacgggcga
      961 cgtgacggta ttcgccaagt ggtcggcggc ctgcgtcaac gtggctggcg gcggagtgat
     1021 caacggctat acgaacggct tctactggac cgcggacagc agcaactgcg cgggcggggc
     1081 cacgatcgtc aatggcgact atggcccggt gaccaccggc aacgtcgtgc tgcccgcgac
     1141 gttcaccgac gtcggtaaca gcagtgacgg cgacaacgtc tacaaggtca ccgcgatcgc
     1201 cgacgacgcc ctgcagttcc gcagcatcac ggcgatcacg ctgccgccga ccctgcgcag
     1261 catcggtgcc aacgccctgt ggtacaactc gctgacgtcg ctgacgctgc cacaatcggt
     1321 gacctcgatc ggcacggagg cattcagcgg caaccagatc acgtcgatca cctggccgag
     1381 cggccccgtc accctcggcg acagcgtctt ctcgtcgaac aagctggcgg cgctgaccct
     1441 gccgtccggg ttgaccgcca tcccgaagtt caccttctcg aacaacgtgc tcaccagcgt
     1501 gacgttgccc gagggcctga tcagcgtcgg cgagcaggcg ttcgcgtcga acaagctcac
     1561 gtcgctgacg ttgccgcaga ccgtgaccac gctggggttc gcggcgttcg tcaacaaccc
     1621 gatcgtcagc gtgaacatcc cggcgtcggt cacctcgttg gccgtcaacg tggtcaagca
     1681 cagcgcgctc aagtcggtgt tcttcgaagg cgcaccgccg gccaccttcg ccgtgggtgc
     1741 cgtcaacacc gacggctcgt tcggtttcgg caccaacctc accggcacat ccggaccggt
     1801 gctgtacttc cgtccgcagt tcagcgagct ggtctacccc ggcggtttca gcacgccgca
     1861 gtggaagggg taccgcactg cctacgtgcc ctcggcggtc gacaccgtct cgcccggtcg
     1921 ctactgggac acgcgcaacg aaccgacctt cgacgggcgg ttccgcaaca cgggtcgcct
     1981 cgctggtggg cagagctttg cgatcgagat cagaggccgc ggcaacgtgg ctggcgacgc
     2041 gaccggcgtg atcgccaacc tgacggtgat cgacgccgcg ggtgcgggct acgccacgct
     2101 gtacccctgc gggggtacgg tgccgaccgc ctcgacggtc aactacacca ccggcgcgat
     2161 cgtggcgaac aacgcgttcg tgccattggc cgccaacggc acggtgtgcg tcttcaccag
     2221 caacnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngt
     2281 gggcatcacc ccggcccgct acctcgacac ccggggcggt tcggacacca ccttcgacgg
     2341 ggcgttttcc ggtggcggcg tggtctacgg gaccaccaag acggtgcaga tcgccgggcg
     2401 cggcaacgtc gccgctggtg ccaaggcggc attcatcaac ctgacggtgg tcaacaactc
     2461 gacgctgcct ggcttcgtca ccgtctatcc gtgtgacgcg ccgcggccga ccgcatcgac
     2521 gctcaacgtc ggtgcgtacg gcgtcgtcgc caacggggcg atggtcaagc tcgcagcgga
     2581 cggcacgctg tgcctgttct cgtccacgtt gaactacctc gtcgtcgacg tcaccgggta
     2641 cgtcccggcg ggcagcgatt cgatcagccc gctggtgccg tctcgtctgt tcgactcacg
     2701 tgccggtgag ccgatcgtcg atggcgtcgg cgccggcccg cgtcttgctg ctggttcgac
     2761 cgtcgaagtg caggtgaccg gacgggtgaa catcccggtg acagcgactg cggcgttcct
     2821 caacgtcgtc gtcgtcagcc ccgacggacc tgggttcgcc acgctgtatc cgtgcggcac
     2881 gttgccgggt accagcagcg tcaactacac gaccggcacg atcgtggcca acaacgcgac
     2941 gacgatgctg tcgccgaccg gatcggtgtg catctacgtg atgcgcggca ccgacgtcgt
     3001 gctcgacgtc accggctaca tcgaatagcg ccagactctc tccgtcgccg cagttcgtcg
     3061 tcgtcgtcac ggggccgccc gggacaggga agtccgcggt gtccgagcac gccgctcggc
     3121 acctcggtgc aaccctgttg gcgtgggact ggttggccgg tggactggcc agtctggcgc
     3181 cgatggcgga tgcgctcgcc tcgttggagc ggccggtgta tcgcgacgcg ggctgggccg
     3241 ggttgtggaa cctgggcgag caccagttgc gcatcggcgg cagcgtcgtg ttcgacggcg
     3301 tggcccgcgt accccagttg gcccgcgccg ccgacctcgc cctccagtac gacgcccgcc
     3361 tgctgacggt cgcgaccacg tgcagcgacg tcgaggtaca cgaacagcgc atcaccggtc
     3421 gccagcgcaa catccccggc tggcacgagt tggagtggcg ctacgtcgag cgcttcctcg
     3481 gcgaatgggt ccctccaccg accgacctga ttctcgatgc agtcgagtcg ctggatcaca
     3541 atttggctcg tgtcgacgcc cgcctcgcca ccctgggcta gacccaccga caccgtgctc
     3601 tggctgtgtc gtcagtgcgc ggggacgagt tcgatcgttt cggcgaggcg gaagcccatc
     3661 gcgtagtggg gtgagcggta ccagccatgg cggcgggcgc agcgggccag gtcgccggcg
     3721 agagcgaagc tgccgccgcg cgcgacccgg tagtgctcgc cgtcggtggc gaccagatcg
     3781 tcggccacgg tcggaccacc gggatatgga cggtaggtat cggacacgta ctcctcgacg
     3841 ttgcctgcca tgtcggacgc gccgaacggc gagtcgccct ccgggtagat gccgatcggg
     3901 gttgtcgtca gcggcccggc ctcggcggtg ttggcgcgag ccgggttcca ctcgtcgccc
     3961 cacggatact cgcgtccgtc gccaccggtc gcggtcaact cccattcggc ctcggtcggt
     4021 aggcggaagt ggcggccggt gcgctcggac aaccaggcgc agtacgcgtc ggcgtcgctc
     4081 ggcgccaccg tgtacacagg ttggttggct gcgccgaggg gaaacgtgcc gtgcccccac
     4141 gagctcgggc ggccggggta accggtggcg agcacgaagt cgaggtactc ctcgttggtc
     4201 accgggtagc ggccgaggcg gaacgcggcc accgtgaccg tgtgccgcgg gacctccttg
     4261 tcgatccagg cccgttcgac accgacgttc gcccagcggg cgagcacgcg atcgacgtcg
     4321 gccgcggcgg ttcccatcgg ataggtcgtc gcgggaacgt cgatcatcgg tgggtcgaag
     4381 gtgtcgatcc gcgggtcgcc gccgatggcg agcagcaacc ctgcgcggtg ccggcgcggc
     4441 cacggctgcg cggggtcctc gacgatcgcc accagcgcct cggcccgcgt cgtaccggga
     4501 tcgaactcgg ttgcccggat gcggcgcagc aatgcccccg acccggccag atcagcctcc
     4561 ggctccaccc ccacgaccgc cgccgcagca gcgaccgtac tcgtcggttc tctgttcgag
     4621 gcctcgttca cgacacgcca gcctacgaac gccccgcccg gccccgctca gccgacccgc
     4681 agccccgagc ggtgaatccg gcttccgcgg aggggtgggc acggggctgg caggatgggt
     4741 gggtgcatca tctcggactg gacgcgatcg tcgatcttcc gccggagttg aacgggggcc
     4801 tcacgttcca cctcccgctg gaggtgttcg cgctggagca tgccgcggcg tcggcggcgc
     4861 acatcgatgc acggatcgaa gcggagttgg cggtcgcatt ccgcgatggg ctcgtcgatc
     4921 cgccgttcta ccgctgggga atcgagcagt accgtccgct cgcgacgacg ttgttggcga
     4981 gtgacgccgc ggcgcggtgc caggccgtgc agagcgtggc accgctgggc gacggattgg
     5041 cgggagcggc attccacggg ttgatccggc tcgggttcgg tgccctgcgt cgcgatgcgg
     5101 acgagattgc acgcgggctg gcgtatctg
//
LOCUS       DAORNF010000149         5117 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_12082_length_5117_cov_1.505136, whole genome shotgun sequence.
ACCESSION   DAORNF010000149 DAORNF010000000
VERSION     DAORNF010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 5117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5117
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12082_length_5117_cov_1.505136"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1260
                     /locus_tag="PLV68_07305"
     CDS             <1..1260
                     /locus_tag="PLV68_07305"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRE01091.1"
                     /translation="DTTTATTVRPVVTQPPGPGFTIPPPPGDGTAWTVDTSRCADLAA
                     ATTVIDDTFVIGTTAPQSGDLSETIYAPVVEGMRAYIAQANRDDVLGSVRVELQVADD
                     KGQAELSPVAVDGLADAGADVIAAMAGVANNLAVRDYLSDNCIPQVMSLATSSRLGEA
                     RTYPWTMGGLVTDTLEITAFADAIRRSTGEGATLGIVTVEDADGDAYAAAVAAAAGEN
                     DLRIVVRQTVLPGVFDAPVPQARVLASSAPDAIIASVNGPTCATLLTELAVVIRTTRN
                     WNPDIYLSSACADPTVLQLAGSAADGVLTASTMGAPTDEFIETMAARGVTTGITRAQL
                     GWTMAEVTVAIAARAQQSDVGLTRASFIEAARALAYTPEFSRPGIEYVTDGLADAFPV
                     ESLQIVRYDARSHGFADVGDLIVQFES"
     gene            1291..1950
                     /locus_tag="PLV68_07310"
     CDS             1291..1950
                     /locus_tag="PLV68_07310"
                     /inference="COORDINATES: protein motif:HMM:NF025343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRE01092.1"
                     /translation="MTRRVPIPRSAGRPKDADSAETRAKLLEGARRIFNRDGYAATTN
                     RNVAAQAGITPAAIYHYFASKAELYAAVVVDVDRLVHERFAPAARTDGSLAERFSRVL
                     EAAHELDMEDPSFAGFVVAIPTEVRRNPELVALIPVDRVRTIPVVSELVADAAAAGEF
                     FDGVNPGDVEDLLSAVLSGLTVMTRFDDGNPRDSNAFRALRRFLSGTLVHSNPVGGLR
                     R"
     assembly_gap    2023..2032
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2033..>3957
                     /gene="pgi"
                     /locus_tag="PLV68_07315"
     CDS             <2033..>3957
                     /gene="pgi"
                     /locus_tag="PLV68_07315"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF001211.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HRE01093.1"
                     /translation="SAATAELAAEWMHRHIMSVVRRDKIFRVAFSGGGTPIAMLHALA
                     ARDIPWRNVEIFQVDERVAPDGDRDRNATHLTAPLLDRIDIPARNVHLMPVTSRSLDR
                     AAREYGRLVTAHPLDVVHLGIGDDGHTASWPPGDPVVNDPAPVAVTAAFNGRVRMTLT
                     PRIVNAARMRMLLAAGVGKAGPLAQWMQRRDLPIQHVSRRRTYLFLDRAAASQIPGRS
                     GSISAGPTTSTNGGHVDVTKTAAWTRLLRTAQTRPVAPLRKLFAADAQRAQRLTFQAA
                     DLRVDLSKQRVTAEVVTRLLAVAEAAQVAARRDAMFAGERINTTENRAVLHTALRAPR
                     SAVIETDDGSGDGTRHDVVPGVHAVLDQMAAFADRVRAGEWIGHTGQAMRTVVNIGIG
                     GSDLGPAMAYVALRAFAQPGLTCRFVSNVDGADIAENLADLDPATTLFVISSKTFTTI
                     ETLTNATTARDWLLAGLGGDRAAIAKHFVAVSTNAAEVAAFGIDTDNMFGFWDWVGGR
                     YSVDSAIGLALMIAIGPERFREFLAGFRAIDEHFLTAPFDRNVPVLMAMIGIWNTNVL
                     GAQSYAVLPYAHELRRFAAYLQQLDMESNGKGVHLDGSPVAIDSGPVVWGEPGTNGQH
                     AFYQLLHQGTRIVPVDM"
     assembly_gap    3958..4000
                     /estimated_length=43
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4001..>4286
                     /gene="pgi"
                     /locus_tag="PLV68_07320"
     CDS             <4001..>4286
                     /gene="pgi"
                     /locus_tag="PLV68_07320"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012562.2"
                     /note="functions in sugar metabolism in glycolysis and the
                     Embden-Meyerhof pathways (EMP) and in gluconeogenesis;
                     catalyzes reversible isomerization of glucose-6-phosphate
                     to fructose-6-phosphate; member of PGI family; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HRE01094.1"
                     /translation="LLTANLFAQTEALAFGKTAAEAAAEGIAPAQLPHRTFSGNRPST
                     TIVAQRLTPRVLGELIALYEHKVFVQGCVWGINSFDQWGVELGKALANRIT"
     assembly_gap    4287..4403
                     /estimated_length=117
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4662..4823
                     /estimated_length=162
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4902..>5117
                     /locus_tag="PLV68_07325"
     CDS             4902..>5117
                     /locus_tag="PLV68_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01095.1"
                     /translation="MRTDASWGQEGLWKLGAGAERPSRAVAATVPVTRGEDEMKLDFT
                     GKKAIVCGGSRGIGKAIAMGFAACGGDV"
BASE COUNT          751 a         1663 c         1611 g          760 t
ORIGIN      
        1 gacaccacca cggcaacaac ggtgcgaccc gtcgtcaccc agccacccgg accaggcttc
       61 accattccac cgccgcccgg cgacgggacc gcgtggacgg tcgacacgtc gcgctgcgcc
      121 gaccttgccg cggccacgac ggtcatcgac gacacgttcg tgatcggcac caccgcgccg
      181 cagtcgggcg acctgtccga gacgatctac gcgcccgtgg tcgaaggcat gcgtgcctac
      241 atcgcccagg ccaaccgcga cgacgtgctc ggctcggtgc gcgtcgagtt gcaggtggcc
      301 gacgacaagg gccaggccga gctgtcgccc gtcgccgtcg atggtctggc cgacgccggc
      361 gcggatgtga tcgccgcgat ggctggcgtg gccaacaacc tcgccgtgcg cgactacctc
      421 tccgacaact gcatccccca ggtcatgtcg ctggccacct cgtcccgtct gggtgaggcg
      481 cgcacctatc cctggaccat gggtgggttg gtgaccgaca cgttggagat caccgccttt
      541 gccgatgcca tccggcgcag taccggcgag ggagcgacgt tgggcatcgt cacggtcgag
      601 gatgccgacg gcgacgcgta cgccgcagcc gttgccgcag cggcgggcga gaacgacctg
      661 cggatcgtcg tccgccagac cgtcttgcca ggcgtgttcg acgcgcccgt tccccaggcc
      721 cgcgtcctcg cttcgtcggc accggacgcg atcatcgctt cggtcaacgg ccccacgtgt
      781 gcgaccttgc tgaccgagtt ggcggtcgtg atccgcacca ctcgcaactg gaaccccgac
      841 atctacctgt ccagcgcgtg tgccgatccg acggtgctgc aactggccgg gagcgcagcc
      901 gacggggtgc tgaccgcgtc gacgatggga gcccccaccg acgagttcat cgagaccatg
      961 gccgcccgcg gtgtcaccac cggcatcacc cgcgcccaac tcggctggac gatggccgag
     1021 gtcacggtgg cgattgccgc ccgggcgcag cagagcgacg tcgggttgac gcgagcgtcg
     1081 ttcatcgaag ccgcccgcgc gctcgcctac acgcccgagt tctcccgtcc cggtatcgag
     1141 tacgtcaccg acgggttggc cgatgccttc ccggtcgagt cgctgcagat cgtgcgctac
     1201 gacgcccgct cgcacggctt tgccgatgtc ggtgacctca tcgtccagtt cgaaagctga
     1261 acgacgccgc tcggtaacgt gagaccaccc atgacgaggc gagttcccat accgcggagc
     1321 gctggtcggc cgaaggacgc cgactcggca gagacgcgcg ccaagctgtt ggaaggcgcc
     1381 cggcggatct tcaaccgtga cgggtacgca gccaccacca accgcaacgt cgccgcgcag
     1441 gcgggtatca ccccggccgc gatctaccac tacttcgcgt ccaaggccga gctgtacgcc
     1501 gctgtggtcg tcgacgtcga tcgtctcgta cacgaacgct tcgctcctgc agcccgcacc
     1561 gacgggtcgt tggccgagcg gttcagcagg gtgctcgaag cagcccacga gctcgacatg
     1621 gaagacccgt ccttcgccgg cttcgtcgtc gccattccca ccgaggtgcg ccgcaacccc
     1681 gagttggtcg ctctgatccc ggtcgatcgg gtgcgcacca ttccggtcgt cagcgagttg
     1741 gtggccgatg cggccgctgc cggcgagttc ttcgacgggg tcaacccagg cgacgtcgag
     1801 gacctgctgt cggcggtgct gtcgggcttg acggtgatga cccgtttcga cgacggcaac
     1861 ccccgtgaca gcaacgcctt ccgcgccttg cgccggtttt tgtcgggcac gctcgtgcac
     1921 agcaacccgg tgggtggcct gcgtcgttga ggggttccga cggcggcacc aagcggagtt
     1981 gtgtggagcg aatagcttgg cgcgatggac atcgtggagg ggnnnnnnnn nnattcagcc
     2041 gcaacagctg aactcgcagc cgagtggatg catcgtcaca tcatgtcggt cgtgcggcgg
     2101 gacaagatct ttcgcgttgc cttcagcggc ggtggcacac ccatcgcgat gttgcatgcc
     2161 ttggccgccc gcgacatccc gtggcgcaac gtcgagatct tccaggtcga cgagcgggtt
     2221 gctccagacg gagatcgtga tcgcaacgca acccatctga ccgcgccgct gctcgaccgc
     2281 atcgatattc ccgcccgcaa cgtccacctc atgccggtga cgtcgcgatc gctcgatcga
     2341 gcagcccgag agtacggtcg cctggtcacc gcccacccgc tcgacgtcgt tcatctcggc
     2401 atcggcgacg acggtcacac cgcgtcctgg ccgcccggtg atcccgtggt caacgacccg
     2461 gccccggtcg cagtgaccgc ggcgttcaac ggccgggtgc ggatgacgct cacgccgcgc
     2521 atcgtcaacg ccgcccggat gcggatgctg ctggccgcgg gcgtcggcaa ggctggaccg
     2581 ttggcgcagt ggatgcagcg tcgggacttg ccgatccaac acgtctcgcg gcgccgtacg
     2641 tatctgttcc tcgatcgggc ggcggcgagc cagatacccg gtcggagcgg atccatctcg
     2701 gccggaccga cgacgtccac gaacgggggg cacgtggatg tcaccaagac cgcggcgtgg
     2761 acgcggttgt tgaggacggc ccagaccaga ccggtcgcgc cgctgcgcaa gctgttcgca
     2821 gccgacgcgc agcgggcgca acggctcacc ttccaggctg ccgatctgcg tgtcgatctg
     2881 tcgaagcagc gcgtgaccgc cgaggtcgtc acacggctgt tggcggtggc cgaggcggca
     2941 caggtcgccg cccgacgcga cgcgatgttc gctggcgagc ggatcaacac caccgagaac
     3001 cgcgccgtgc tgcacaccgc gttgcgcgcg cctcggtcgg cggtgatcga gaccgacgac
     3061 ggctccggcg acggcacccg gcacgacgtc gtgcccggcg tgcacgcggt gctcgaccag
     3121 atggccgcgt tcgccgaccg ggtgcgtgca ggcgagtgga tcggtcacac cggccaggcg
     3181 atgcgcacgg tggtcaacat cggcatcggt ggcagcgacc tcggcccggc gatggcttat
     3241 gtcgcgctgc gcgccttcgc gcagccgggt ttgacgtgcc ggttcgtgtc caacgtcgac
     3301 ggcgccgaca tcgccgagaa cctggccgac ctcgacccgg cgacgacgct gttcgtcatc
     3361 tcgtccaaga ccttcaccac gatcgagacg ctgaccaatg cgaccaccgc tcgcgactgg
     3421 ttgctggccg gccttggcgg cgaccgggcg gcgatcgcca aacacttcgt cgccgtgtcg
     3481 accaacgccg ccgaggtcgc cgcgttcggc atcgacaccg acaacatgtt cggcttctgg
     3541 gactgggtcg gcgggcgcta ctcggtcgac tcggcgatcg ggctggcact gatgatcgcg
     3601 atcggcccgg aacggttccg cgagttcttg gccggctttc gagcgatcga cgagcacttc
     3661 ctgaccgccc cgttcgatcg caacgtgccg gtgctgatgg cgatgatcgg catctggaac
     3721 accaacgtgc tcggcgccca gagctacgcc gtgttgccgt acgcccacga actgcgccgc
     3781 ttcgccgcct acctgcagca gctcgacatg gagagcaacg gcaagggcgt ccacctcgac
     3841 gggtcaccgg tggcgatcga cagcggcccc gtggtgtggg gcgagccggg taccaacggt
     3901 cagcacgcct tctaccaact gcttcaccag ggcacccgga tcgttccggt cgacatgnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cctgttgacg gccaacctgt
     4021 tcgcccagac cgaggcgttg gccttcggca agaccgcggc ggaagcggcc gccgagggca
     4081 tcgcccccgc gcaactgcca caccgcacgt tcagcggcaa ccgtccttcg accaccatcg
     4141 tcgcccagcg cttgacgccg cgggtgttgg gcgaactgat cgcgctgtac gagcacaagg
     4201 tgttcgtgca gggctgcgtg tggggcatca acagcttcga ccaatggggg gtcgagctcg
     4261 gcaaggcgct ggccaaccgg atcaccnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4381 nnnnnnnnnn nnnnnnnnnn nnncgtcgtc ggactcggcc gcggcgtcgg tggcgggcgg
     4441 tgtcatcgac agcgtcagcc cgtcgatctc gagcacgatc tcaggttgcc ccgacgtgtt
     4501 ggtgacgacc gcaggctccg tggcacgact ggtgataccc atgcgaggca cggtaacgcg
     4561 atgtgagagc gctacggtgc acgggcgata tggccgatcc gttggagcga ctgaccaacc
     4621 tggtggcgct gctgctggag acccgccggc ccttgtcgct gnnnnnnnnn nnnnnnnnnn
     4681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4801 nnnnnnnnnn nnnnnnnnnn nnngaccggc gccgctacga gctggccgat ctcgaccttg
     4861 ccgacgacga gcgccacgcc ctgcaacttg ccgtcgccgc cgtgcgcacc gacgcgtcgt
     4921 gggggcagga gggcctgtgg aagctcggcg ccggcgccga gcgtccgtcc cgggccgtgg
     4981 ccgccacggt gccggttaca agaggagaag atgaaatgaa gctggatttc acgggcaaga
     5041 aggcgatcgt ttgcggcggc agccggggca tcggcaaggc gatcgccatg ggcttcgcgg
     5101 cctgcggcgg cgacgtg
//
LOCUS       DAORNF010000150         5088 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_12194_length_5088_cov_1.839062, whole genome shotgun sequence.
ACCESSION   DAORNF010000150 DAORNF010000000
VERSION     DAORNF010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 5088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5088
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12194_length_5088_cov_1.839062"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="PLV68_07330"
     CDS             <1..226
                     /locus_tag="PLV68_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01096.1"
                     /translation="AGYEQVIDVSKIYFKPEAEAAAASLGAVMGIESVTRMPTPPPIV
                     GALDTLGDATLLIMLGKDLGGKDVPGFAGR"
     gene            complement(334..969)
                     /gene="udk"
                     /locus_tag="PLV68_07335"
     CDS             complement(334..969)
                     /gene="udk"
                     /locus_tag="PLV68_07335"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HRE01097.1"
                     /translation="MGGQTGAAPFLIGLAGGTCSGKTTFAERLAEANGSDQVALIKLD
                     SYYKRKTRAPGEDRIETNYDHPDAFDWPLLNDHLAALKAGATIPVPVYDYTIDDRTDE
                     VRMVAPCRIVVMEGILVLYEPTLRERFDLRVFIDVDADIRLIRRIHRDIAERGRSLDS
                     VISQYLATVRPSHEHFIEPSKRHADVILPLGGENHAALALLTARIGQLLED"
     gene            1047..1328
                     /locus_tag="PLV68_07340"
     CDS             1047..1328
                     /locus_tag="PLV68_07340"
                     /inference="COORDINATES: protein motif:HMM:NF023092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3263 domain-containing protein"
                     /protein_id="HRE01098.1"
                     /translation="MAEQTELTERERALLEFEGTWWTLDDAKEDLIRARFACSLDDYY
                     QELNRLIEVPAALAHDPLVVRRLLRGRDRRRRARLDSPAKVDDARGGPH"
     gene            1328..1984
                     /locus_tag="PLV68_07345"
     CDS             1328..1984
                     /locus_tag="PLV68_07345"
                     /inference="COORDINATES: protein motif:HMM:NF024791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HRE01099.1"
                     /translation="MSNDTPSRRPTGKSSSGAPMGSTVSIVIAVIAVVVGFLILKNIN
                     DGGGGGTTGGNNNTTTTTTDPTTNTEDIANTTTTSEVSTVDKDPNQIVVVANASGIQG
                     AAGRYTTTLQGEGWTTGAAETAAQRVTASLVYFLPGGENVAASVAAVMSDPAGTPIVA
                     SAMPTPPPLEAGKTLPSGVTVLVMLGPDLADKPLPGLAAAGTSTNTTTTLPGAPAPNG
                     "
     gene            complement(2001..>2814)
                     /locus_tag="PLV68_07350"
     CDS             complement(2001..>2814)
                     /locus_tag="PLV68_07350"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF014634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HRE01100.1"
                     /translation="TVTGPLGDPVEAPWRFSRNVAVIEMALASGLELAGGAAGNDPLA
                     ASTTGTGELIDAALDAGAKKIIVCLGGSATTDGGLGALRAIHGPQRLRGVELLVACDV
                     ETVFADAAVVFGPQKGATPAQVELLTGRLERLVQVYRNDYGVDVSELAGSGAAGGLAG
                     SLVALGATLMPGFDLVADEVDLYDAIATADLVITGEGYLDEQSFNGKVIGGVAEMAGA
                     LGKPVAAIVGDADPAVADRIEHISLVAAFGRERALAATRTCIEDAATMLLRR"
     assembly_gap    2815..2995
                     /estimated_length=181
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3093..3368
                     /locus_tag="PLV68_07355"
     CDS             3093..3368
                     /locus_tag="PLV68_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HRE01101.1"
                     /translation="MAVTVRIPTTMRPLAGGNSTVKVDGATLAEVLKNLNDQHPGFDD
                     RLFDENGALRKFVNVFVADDDVRYLDGINTVVPDGETVSIIPAVAGG"
     gene            complement(3385..4857)
                     /locus_tag="PLV68_07360"
     CDS             complement(3385..4857)
                     /locus_tag="PLV68_07360"
                     /inference="COORDINATES: protein motif:HMM:NF012299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRE01102.1"
                     /translation="MRVVVVGGGVSGLGSALSLARHGHDVVVVERDHTPMPESADEAF
                     DWDRRGAPQVRHSHAFLAKLCLLLRTDYPDIYASLLAEGATELLFGRDLPPEMVEFSP
                     DPGDADLTMLACRRTTFEWVVRKAALAEGRVEFVTGSGVVALVTADSQQGASVPVVAG
                     VRLDNGSEIGADLVVIAGGRRSTAPEWMAAIGTPAVPEEIEDTGIVYLSRFYRLREGQ
                     QYPPRSGAIGGDLGYLKYGVFVGDNDTFSITLAVPTDDDDLRKRLADPEMFDLAAAQL
                     VATEPWVDGRAVPITPSVHVMAGLLNRRRHYVVDGEPLALGVVPVGDAVLCTNPLYGR
                     GCSIAFWAADLLTKAIASTAEESPVARLRSIAIDYDAALCAEILPWYLSSVEQDKEAR
                     RVAAALLAGQDPDTDADDPRAFMRGVFRDGLLPALRSDQVVLRAFMRNLNLLVPPDAM
                     AKDADVGARVLAVWQTRDQRPPLPAMGPRRRAEFAALLPG"
BASE COUNT          810 a         1788 c         1623 g          686 t
ORIGIN      
        1 cgctggctac gagcaggtga tcgacgtcag caagatctac ttcaagcccg aggccgaagc
       61 ggcggcggcc agcctcggcg cggtgatggg catcgagagc gtcacccgca tgcccacccc
      121 tcccccgatc gtcggtgccc tcgacaccct cggtgacgcg acgttgctga tcatgctggg
      181 caaggacctg ggcggcaagg acgtccccgg tttcgccggg cgctgagacg cattgcctgc
      241 agccgcgcca gcccgcggtt cagcaacgcc gttcagcaac caacgcgccg cggccgacag
      301 cggcgatccg cggcgcccgc accagcgcgc ggttcagtcc tccagcagtt ggccgatccg
      361 cgcggtcagc agcgccagcg cggcgtggtt ctcgccgccc agcggcagga tgacgtcggc
      421 gtggcgcttg ctgggctcga tgaagtgctc gtggctgggc cgcaccgtgg ccagatactg
      481 cgagatcacg ctgtcgaggc tgcggccgcg ctcggcgatg tcacgatgga tgcgccggat
      541 caggcggatg tcggcgtcga cgtcgatgaa cacgcgcagg tcgaaccgct cgcgcagggt
      601 cggttcgtag agcaccaaga tgccctccat caccacgatc cggcacggcg cgaccatccg
      661 cacctcgtcg gtgcggtcgt cgatcgtgta gtcgtaaacc ggcaccggga tcgtcgcccc
      721 cgccttcagc gcggcgaggt ggtcgttcag caacggccag tcgaacgcgt cgggatggtc
      781 gtagttggtc tcgatccgat cctcgccagg ggcgcgcgtc ttgcgcttgt agtacgagtc
      841 gagcttgatc agcgcaacct ggtcgctgcc gttggcctcg gccaaccgct cggcaaaggt
      901 ggtcttgccc gaacacgtcc cacccgccag cccgatcaag aacggcgccg cccccgtctg
      961 tccccccacc acgctcaccc ggccaagcta cgcgatctac cgcggggatg cttgtttcgc
     1021 ggggcatcac caggcaggct gtgggagtgg ctgaacagac cgagctgacc gaacgcgagc
     1081 gcgccctgct ggagttcgag ggcacgtggt ggaccttgga cgacgccaag gaggatctga
     1141 tccgcgcgcg gtttgcctgc agcctcgatg actactatca ggaactcaac cggctgatcg
     1201 aggtgccggc agcgctcgcc cacgacccgc tggtggttcg ccgcctgctg cggggccgtg
     1261 atcggcgtcg ccgggcccgc ctcgatagcc ccgccaaggt ggacgacgca cgcggaggac
     1321 cgcactgatg agcaacgaca cgccgagccg tcggccgacc ggcaagtcaa gcagtggcgc
     1381 cccgatgggg tcgaccgtca gcatcgtcat cgcggtgatc gcagtcgtgg tcgggttctt
     1441 gatcctgaag aacatcaacg acggcggcgg tggcggcacg actggtggca acaacaacac
     1501 gaccacgacg accaccgatc cgaccaccaa caccgaggac atcgccaaca ccaccaccac
     1561 gtccgaagtg tccaccgtcg acaaggaccc gaaccagatc gtggtcgtcg ccaacgcttc
     1621 cggcatccag ggtgcggccg ggcgctacac cacgacgctg cagggcgagg gctggaccac
     1681 gggcgccgcg gagaccgctg cccagcgcgt caccgccagc ctggtgtact tcctgcccgg
     1741 cggcgagaac gtcgccgctt cggtcgccgc ggtcatgagc gatccggcgg ggacaccgat
     1801 cgtcgccagc gcgatgccca ccccgccacc gctcgaagcg ggcaagacct tgcccagcgg
     1861 cgtcaccgtg ctcgtcatgc tcggtcccga cctggccgac aagcccctgc caggcctcgc
     1921 cgccgctggc acctcgacca acacgaccac caccctgccg ggagcccccg cccccaacgg
     1981 ctgaccgctt tccaaccgga tcagcgacgg aggagcatgg tggccgcgtc ttcgatgcac
     2041 gtgcgcgtcg cggcgagggc acgctcgcgg ccgaaggccg cgaccaatga gatgtgttcg
     2101 atccgatcgg caaccgccgg gtcggcatca ccgacgatcg cggccactgg cttgcccaac
     2161 gcgccagcca tctcggcgac gccaccgatg accttgccgt tgaagctctg ctcgtcgagg
     2221 tagccctcgc cggtgatcac caggtcggcc gttgcgatcg cgtcgtagag gtcgacctcg
     2281 tcggcgacga gatcgaagcc gggcatcagc gtggccccga gcgccaccag cgagccggcc
     2341 aacccacccg cagccccgct cccggcaagc tcgctgacgt cgacgccgta gtcgttgcga
     2401 tacacctgca ccagccgttc gagccgcccg gtcaacagct cgacctgggc cggggtcgcc
     2461 cccttctgag ggccgaacac gaccgccgcg tcggcgaaga ccgtctcgac gtcgcaggcc
     2521 accaatagct cgacgccacg cagccgttgc ggaccgtgaa tggcccgcaa cgcgccgaga
     2581 ccaccatcgg tggtagccga gccaccgagg cagacgatga tcttcttggc cccggcatcg
     2641 agcgcggcgt cgatcaactc acccgtaccg gtggtggatg ccgcgagcgg gtcgttgcca
     2701 gccgcgccgc cggccagttc gagaccgctg gccagcgcca tctcgatcac ggccacgttg
     2761 cggctgaacc gccagggcgc ctcgaccggg tcaccgagcg ggccggtcac cgtgnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncccgg
     3001 ggccgctggc gccagccggg tgacgacggt tcgtcgaagg gccggccgag acgacggtgg
     3061 cgaatcccga ccacatcggt agtgtttctg gcatggctgt gaccgttcgt atccccacca
     3121 ccatgcgtcc cttggccggc ggcaactcga cggtcaaggt cgacggcgcc acgttggccg
     3181 aggtgctcaa gaacctcaac gatcagcacc ccggcttcga cgatcggctg ttcgacgaga
     3241 acggcgcgct gcgcaagttc gtcaacgtgt tcgtcgccga cgacgacgtg cgttacctcg
     3301 acggcatcaa caccgtcgtg cccgacggcg agaccgtcag catcatcccc gccgtcgccg
     3361 gcggctgatc actgcaacag cgggtcaacc gggcagcagg gccgcgaact cggcgcgtcg
     3421 gcgcggcccc atcgcgggca ggggcgggcg ttgatcgcgg gtctgccaca ccgccaacac
     3481 ccgcgcgccg acgtcggcgt ctttggccat cgcatccggc ggcacgagca ggttgaggtt
     3541 gcgcatgaac gcccgcaaca cgacctgatc gctgcgcagg gccggcagca acccgtcccg
     3601 gaacacgccg cgcatgaacg cccgcggatc gtcagcgtcg gtatccgggt cctgcccggc
     3661 caacaacgcc gccgcgacgc ggcgagcctc tttgtcctgc tcgaccgagg acagatacca
     3721 cggcaggatc tcggcacata gcgctgcgtc atagtcgatg gcgatcgagc gcaggcgggc
     3781 gaccgggctt tcctcggcgg tggacgcgat cgccttggtc aacagatcgg cagcccagaa
     3841 cgcgatgctg cacccacgcc cgtagagcgg gttggtgcac aacacagcat caccgacggg
     3901 cacgacgccg agcgccagcg gctcaccgtc caccacgtag tggcggcgac ggttgagcag
     3961 cccagccatc acgtgcacgc tcggcgtgat cggcacggct cggccatcga cccacggctc
     4021 ggtcgcgacg agttgcgctg cggcgaggtc gaacatctcc gggtcggcga ggcgcttgcg
     4081 gagatcgtcg tcgtcggtcg gcacggcgag ggtaatgctg aacgtgtcgt tgtcgccgac
     4141 gaacaccccg tacttgagat agccgagatc gccgccgatc gcgccgctgc gcggtggata
     4201 ctgctggccc tcccgcaacc ggtagaagcg gctgaggtag acgatgccgg tgtcctcgat
     4261 ctcctccggc accgccgggg tgccgatcgc ggccatccac tccggggccg tgctgcgccg
     4321 cccaccggcg atcacgacca gatcggcgcc gatctcgctg ccgttgtcca accgcacgcc
     4381 agccaccact gggacggagg cgccctgctg gctgtcggcg gtcacgagcg cgacgacgcc
     4441 gctgccggtc acgaactcga cccggccctc ggccagcgcg gctttgcgga ccacccactc
     4501 gaaggtcgtg cgacggcagg cgagcatcgt cagatcggcg tcgcccggat cgggggagaa
     4561 ctccaccatc tccgggggca ggtcgcgccc gaagagcaac tcggtcgcgc cctcggccaa
     4621 cagcgaggcg tagatgtccg ggtaatcggt ccgcaacaac aggcacagct tggcgaggaa
     4681 ggcgtggctg tggcgtactt gcggcgcgcc ccggcgatcc cagtcgaacg cctcgtcggc
     4741 gctctccggc atcggtgtgt ggtcccgctc gacgaccacc acatcgtgtc cgtggcgggc
     4801 gagcgacaac gcgctcccca ggcctgaaac cccaccgccg accacgacca ctcgcatggc
     4861 gcgccaacgt agacgccacc accggtgcca ccaccaaccg agccgacccg cagcgcgccg
     4921 ctcccgcgct cacccgtgga ggtcagcagg tcggttcgga cggcgcctcg accgggtcgg
     4981 tgccggtgtc cctcgacggc gagcgcgtcg gagcgagcca ggccgcccgc gccggggagc
     5041 tcatcagcgt gtcccggtcc caccactcca gcgccgcggt gcggctga
//
LOCUS       DAORNF010000151         5077 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_12240_length_5077_cov_1.573676, whole genome shotgun sequence.
ACCESSION   DAORNF010000151 DAORNF010000000
VERSION     DAORNF010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 5077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5077
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12240_length_5077_cov_1.573676"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>356)
                     /locus_tag="PLV68_07365"
     CDS             complement(<1..>356)
                     /locus_tag="PLV68_07365"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01103.1"
                     /translation="STLVFPDELVEPASLDGFDVLDDVSVSDKELVMARSLVEAMSDD
                     FVGDRYQDEYRLAVEQIIEQKAAGKPIVVADEPAPDRSNVIDLAAARGPYIDQSQSLN
                     LFMESPNIGRLSSMYM"
     assembly_gap    357..431
                     /estimated_length=75
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<432..808)
                     /locus_tag="PLV68_07370"
     CDS             complement(<432..808)
                     /locus_tag="PLV68_07370"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HRE01104.1"
                     /translation="MVSIPIKLLPAVKRKSISFNQIDDQTMSRVRYRKVSEATGDEVP
                     NDHIVKGFDLGGENYVLITDSDLAPLQPSKSREINLESFVLVDDINPLMYDSSYLVMP
                     DKTVKPYALLASALDGTRRVGIGR"
     gene            complement(923..2779)
                     /locus_tag="PLV68_07375"
     CDS             complement(923..2779)
                     /locus_tag="PLV68_07375"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HRE01105.1"
                     /translation="MAYSMWRNSRQTAALRGAAVSVRELGRLSEQLATADDRERMIAT
                     ILERAGTMVGARQVSVALWSGDDTALLSRSSDRSDVSGWGGSEVPVERDDLGPLADAL
                     HPVVVTPDLSSPIGTALVTDAPVLLGDAAAVRATFPAGWEGSGPDPASAVALVLRRPN
                     GRSLGAIEWVWARGRELTPATRSTLLTVAEVCEQSLFRAEVHHGRWRAASALASLSQG
                     LSVARTLTEIAELVVRFGPGASGAHHVAVAFTNESLTELTVHHAVVGEDGVVQDPAVA
                     GTSTFSMHIDPNGPLTSMLRRGQPIQLDGMSVFQDVPEVVALIGGRLERTTGIPLIDA
                     EGRLRGAIAFIHLRGISDPREPVYAEAGRLDTIADLTAQTVERSLLYQHEHELVVNLQ
                     QQTLGALPAVDGLCCAARYLPSSSMLGLGGDWYDVYPLDDGGAAVMVGDVAGHGIDAI
                     ADMAEFRTTISTLLRTRSDLSELAALSTSLLAEPGGELRFATVGLMVIDPSRRRLSYI
                     RCGHPPALMCRPDGSVELLDQAGGAPIGIERRSLSAASVAFEPGTVLLAYTDGLIERR
                     DEVLDTGIERLRSALAECPTTDPVAIVDHVIARCLAGRSTEDDTAVLVIRVD"
     gene            complement(2834..>3348)
                     /locus_tag="PLV68_07380"
     CDS             complement(2834..>3348)
                     /locus_tag="PLV68_07380"
                     /inference="COORDINATES: protein motif:HMM:NF015859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHASE domain-containing protein"
                     /protein_id="HRE01106.1"
                     /translation="RCEDQAGINITERGVNGLIAAESRAEYLPVQYVYPVTETSRSVV
                     GFDTASDRDRYAASSAARDLGALSLSPPVPSQPSGQTSVYFAQPLFKPGLALGTVEQR
                     RAAIVGFATGLRPASAILESVMAQLPDGARVRVTDGEQFVAGTAEAPSGGHSSTISVG
                     GRTWVVLKAA"
     assembly_gap    3349..3399
                     /estimated_length=51
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3400..3632)
                     /locus_tag="PLV68_07385"
     CDS             complement(<3400..3632)
                     /locus_tag="PLV68_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01107.1"
                     /translation="MVAILLIGLTASAVLYSRTVRGANDEQAALAQRASRVLQNSTSV
                     LEAALGGASVMVGRDGSVDPAAFAAFARGVVETT"
     gene            complement(3835..4155)
                     /locus_tag="PLV68_07390"
     CDS             complement(3835..4155)
                     /locus_tag="PLV68_07390"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRE01108.1"
                     /translation="MRDVLRRWNLVDGTNDTCELLVSELVSNAVLHGSGEVTVTVMEC
                     DDGGVRVEVRNQGSGRPVLRHPRPNDLSGRGLRLVDELSTRWGLDSRGGTTLVWFEAH
                     LLPV"
     assembly_gap    4575..4584
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4585..4776
                     /locus_tag="PLV68_07395"
     CDS             <4585..4776
                     /locus_tag="PLV68_07395"
                     /inference="COORDINATES: protein motif:HMM:NF040511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPGG-motif small membrane protein"
                     /protein_id="HRE01109.1"
                     /translation="RVRLTTHKPNTLEVRDMEFLLWILAVILVISGIVALTRKEFAWG
                     AVLIIVGLLVGPGGVSVFT"
BASE COUNT          847 a         1691 c         1653 g          750 t
ORIGIN      
        1 acatgtacat cgacgacagc cggccgatat tcgggctttc catgaacagg ttgagcgact
       61 gcgactggtc gatgtacggc ccgcgcgcgg cggccagatc gatcacgttg ctgcgatcgg
      121 gggcgggttc gtccgcgacc acgatcggct tgcccgccgc cttctgctcg atgatctgct
      181 cgaccgcgag gcggtactcg tcctggtagc gatcgccgac gaagtcgtcg ctcatcgctt
      241 cgaccaacga gcgggccatg accaactcct tgtcgctgac cgacacgtcg tcgagcacgt
      301 cgaatccgtc gaggctggcc ggctcgacca actcgtccgg aaaaaccagg gtcgacnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn ncgtccgatg cccactcgcc gggtgccgtc gagcgccgac gccaacaacg
      481 cgtacggctt gacggtcttg tcaggcatga ccaggtacga cgagtcgtac atcagcgggt
      541 tgatgtcgtc gaccaacacg aacgactcga ggttgatctc acgagacttc gacggttgca
      601 acggggccag gtcgctgtcg gtgatcagca cgtaattctc gccgccgagg tcgaaccctt
      661 tgacgatgtg gtcgttcggt acctcgtcac ccgtggcctc gctgaccttg cggtagcgga
      721 cgcgcgacat cgtctgatcg tcgatctggt tgaacgagat gctcttgcgc ttgaccgcgg
      781 ggagcagctt gatgggaatc gagaccattc caaacgagat ggaacctgtc cagaccggcc
      841 gcgccatgac gcgacactac ccgccgccag cactcaccag acccggccac gtcggcggat
      901 cgacccatcg atatcggtcg gatcaatcga cgcggatgac caggacggcg gtgtcgtcct
      961 cggtgctgcg ccccgccagg cagcgtgcga tgacgtggtc aacgatcgcc accggatcgg
     1021 tggtcgggca ctcggccagc gccgagcgca gccgctcgat gcccgtgtcg agcacctcgt
     1081 cacgtcgttc gatcaacccg tccgtgtagg ccagcagcac ggtgccgggc tcgaacgcca
     1141 cggacgccgc gctgagcgag cggcgctcga tgccgatcgg tgcgccacct gcctgatcga
     1201 gcaactcgac cgacccgtcg gggcggcaca tcaaggcggg tgggtggcca cagcggatgt
     1261 agctcaggcg gcgacgcgag ggatcgatga ccatcaaccc gacggtcgcg aagcgcagtt
     1321 cgccaccggg ctcggccagt agcgaagtcg acaacgcggc cagttcggac agatcggatc
     1381 gcgtccgcag caatgtggag atcgtcgtgc ggaactcggc catgtcggcg atggcgtcga
     1441 tgccgtgacc cgcgacgtca ccgaccatca ccgccgcgcc gccgtcgtcg agcgggtaga
     1501 cgtcgtacca gtcgccaccg aggccgagca tcgacgacga gggcaggtag cgggcggcgc
     1561 agcacaaccc gtcgaccgcg gggagcgcac cgagggtctg ctgctgcagg ttgaccacca
     1621 gttcgtgttc gtgctggtac aacagcgatc gttcgacggt ctgcgcggtc agatcggcga
     1681 tggtgtccag ccggccggct tcggcgtaga ccggctcccg cgggtcggag atgccgcgga
     1741 ggtggatgaa cgcgatcgcg ccgcggagcc ggccttcggc gtcgatcagc gggataccgg
     1801 tcgtacgttc gaggcgtccg ccgatcaggg cgaccacttc gggcacatcc tggaacaccg
     1861 acatcccgtc gagctggatc ggctgaccac ggcgcaacat gctggtgagc ggaccgttgg
     1921 ggtcgatgtg catcgagaac gtcgaggtgc ccgcgaccgc cgggtcctgc actacgccgt
     1981 cctcgccgac caccgcgtga tggaccgtca gctcggtgag ggactcgttc gtgaacgcga
     2041 ccgcgacatg atgggctccg ctcgcacccg gcccgaagcg gaccacgagc tctgcgatct
     2101 cggtcagcgt ccgcgccacc gacaacccct ggctcaggct ggccaacgcc gacgcggcgc
     2161 gccaccgacc gtgatggacc tcggcccgga agaggctctg ctcgcacacc tcggccacgg
     2221 tcagcaacgt cgagcgtgtc gcgggcgtga gctcgcgacc gcgtgcccag acccattcga
     2281 tcgcgccgag tgaacgaccg ttcggacgcc gcagcaccaa cgcgaccgcg ctggccggat
     2341 ctgggcccga cccctcccac ccggcgggaa acgtggcccg cacggcagcg gcgtcgccga
     2401 gcagcaccgg cgcgtccgtg accaacgccg taccgatcgg actggacaga tcgggcgtca
     2461 ccacgaccgg atgcaacgcg tctgccagcg gacccaggtc gtctcgttcg acgggcacct
     2521 ccgagccgcc ccaaccgctg acatcgctgc ggtcagagct ccgcgacaac agcgcagtgt
     2581 cgtcgccaga ccacagcgcg accgacacct gacgcgcccc caccatggtt cccgcccgtt
     2641 cgaggatggt ggcgatcatc cgctcacggt catccgctgt ggcgagttgc tcgctcagcc
     2701 ggccgagctc gcggaccgac acggccgcgc cccgcagggc ggcggtctga cgggagttgc
     2761 gccacatcga gtacgccacg gcagcggcct taccgactat gaaggcaaca tcctcgacac
     2821 ggcggggcgg ccgctaagca gctttcagga cgacccacgt ccggccgccg acggagatcg
     2881 tcgaggagtg accacccgac ggcgcctcgg ccgtgccggc gacgaactgc tcgccgtcgg
     2941 tgacgcgcac gcgggctccg tcggggagct gggccatgac cgactcgagg atcgcgctcg
     3001 cggggcgcag acccgtggcg aacccgacga tggcagcccg gcgttgttcg accgtgccca
     3061 gcgcgaggcc gggcttgaac agcggttggg cgaagtagac gctcgtctgt ccggacggct
     3121 gcgagggcac cggcggcgag aggctcagcg ccccgaggtc gcgggccgcg ctcgatgccg
     3181 cgtaccgatc gcggtctgag gcggtgtcga acccgaccac gctgcgcgag gtctcggtga
     3241 ccgggtagac gtactgcacg gggaggtact ccgcccggga ctcggcggcg atcaggccgt
     3301 tgacgccgcg ctcggtgatg ttgatccccg cctggtcctc gcacctggnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng tggtctccac cacgccgcgg
     3421 gcgaaggcgg cgaaagccgc tggatcgacc gaaccgtcgc gaccgaccat caccgaggca
     3481 ccccccagtg ctgcttccag cacgctggtc gagttctgca acacccggct ggcccgctgc
     3541 gccagcgcgg cctgttcgtc gttcgcccca cgcaccgtgc gcgaatacag caccgccgac
     3601 gcggtcaacc cgatcagcag gatggcgacc acgatcaggc ccgctacacc ccgtccgaac
     3661 gaccgcggca gtgaccaacc gttctggccg gaacgatctg cgccggaccg atctgcgccg
     3721 gaacgatcgg gaccggaacg atcgggatcc gccgggtccc tgccacctgt gctgaacgtg
     3781 gaagacgtgc tcgtactcgt gtgcgtctcg ctcatgtaac gactcgcctg gcagtcacac
     3841 cggcagcagg tgtgcctcga accacacgag cgtggtgccg ccacgcgagt cgagccccca
     3901 gcgagtggag agctcgtcga ccaggcgcag gccgcgaccc gacaggtcgt tcgggcgcgg
     3961 gtggcgcagc accggccggc ccgacccttg gttacggacc tcgacccgga caccgccgtc
     4021 gtcgcactcc atcacggtga cggtgacctc ccccgaaccg tgcagcaccg cgttcgacac
     4081 cagttcgctg accagcaact cacacgtgtc gttggtgccg tcgaccaggt tccaccgccg
     4141 cagcacatcg cgcacgaagg cacgcccgga gcggtcgggc tcagccggat ccgttctgtc
     4201 ctcacgttca tcggctctgt ctccacacgt ggtgggccac gtctcgctca ctcgtctggc
     4261 tcagcgcagg gggccgcgca tgctgccacc gcgaccctac acagcgcgca cggacgcgct
     4321 gcacgtgcaa ctcgcgtggg cctgcttgct tcgggggcac gtatcgatga gtgccctcgc
     4381 cgcgcaaccg tcaaacggca cggcatggtg gcaaacgggt tagcgtggtg gcaaacgggc
     4441 acggcatgct ggcaaacggg cacagttcgt tggggacgag atgtttgggg cgagcaagcc
     4501 cggggtatca gcgtggccga gccgaacaat ccaaaccgtt cagcagggcg cgtcactcgc
     4561 acgtcgccag gcggnnnnnn nnnncgtgtt cggctcacaa cccacaagcc gaacacgttg
     4621 gaggtcaggg atatggaatt tctactgtgg attttggcgg tcatcctggt catctcgggc
     4681 atcgtggcct tgacccggaa ggaattcgcc tggggtgctg tgctgatcat cgtcggcctg
     4741 ctcgtcgggc ctggcggggt cagcgtcttc acgtagaatt cgccattcgt gacacaccag
     4801 ctaccggcaa gcagtgaccc ataaacgccg gtagctggtg cgcgtcacgg acgtgcatct
     4861 gatgggcgga gcacctgatg ggcgagcacc tgatggacaa gtaagggagc agggatggaa
     4921 gaggtcggca ggaacgagtc gatccgggtc tggatcgaac gaggcgtcgt acacgccgaa
     4981 ggcgacatcg acctggccgg cggccccgtg ctcgacgctg cgatctctcg cgtcgaagac
     5041 ggcgcggccg tggctatcga cctcgccgga gtcgagt
//
LOCUS       DAORNF010000152         5060 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_12291_length_5060_cov_1.664735, whole genome shotgun sequence.
ACCESSION   DAORNF010000152 DAORNF010000000
VERSION     DAORNF010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 5060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5060
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12291_length_5060_cov_1.664735"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(13..732)
                     /locus_tag="PLV68_07400"
     CDS             complement(13..732)
                     /locus_tag="PLV68_07400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01110.1"
                     /translation="MDVEPVAQSAYVEIDDGSTRWRIEAGFMTSNWTCIWGRGCHGID
                     FTPQAERGLGCCSVGAEIDGVDEAMLVDASAAALEPAIFQFFDVAAADGVFSDEQRVN
                     TRVVDGACIFLNRPGFAGGAGCALHLGALAADESPIDWKPSVCWQLPIKVDWEQHPHD
                     VDREVATLRRWSRADWGAEGETMAWCCTEQISPVFPTLGGEAYCGTAPVIDSLAEELE
                     QIVGTPVFVELRRRLDRPSQP"
     assembly_gap    973..982
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1112..>1654
                     /locus_tag="PLV68_07405"
     CDS             1112..>1654
                     /locus_tag="PLV68_07405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01111.1"
                     /translation="MSSYMGFDPLRLQSLASAMQRDTDELRRLRSSESAAAEPLRACA
                     RIADELEGRWLPLIRNLVGCGVLDGYRPANLDDVSLTHSYLDWLIRSHGFVPVTDPLD
                     WTPSPMTPDHARVVGEWLASRGGEVSGADLDALARMLHAIATSPAASAAFVHGINGTA
                     DLDATIDATRLFTLVVSLSGI"
     assembly_gap    1655..1677
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1678..2953
                     /locus_tag="PLV68_07410"
     CDS             <1678..2953
                     /locus_tag="PLV68_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01112.1"
                     /translation="VQVAVTAALADLGRITAAVGEHEAMATALTTGRAVDPYSAALIV
                     GQLGLTAEMLSATTAALLRREDEEGWVGGPSAGGAVADALFPVNVATPRAATLLLQNL
                     ANEVWLVFTGLDVAATESLVMTGIHGAGVEGAGVEGGSGEGAGVDDATRISLLAPLLD
                     YVTAQIPLPYFSRSDSPWTVETLSHLAAAALAPDLVMFFYPTPDQPQADLGLLGRVYD
                     QLAEAPLLFDLLVRERMQMQATIDWADPASSNAQTELTLVTQLLDDIAVKAKMTEAER
                     GRVWWNLMWWGIGLSSNGVPTLIGEIVVDEVIGLIQELVEDAGYGPMTAEEARDKVLV
                     SQNGDAMWRAATVAGGLYLAGRDAGTFSASDPVPVPDPDAGDDGAGYRARLDAWFDRH
                     PGASANMRAVVEKIWPVKQQAATHNRLLDAAN"
     gene            complement(2988..3215)
                     /locus_tag="PLV68_07415"
     CDS             complement(2988..3215)
                     /locus_tag="PLV68_07415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01113.1"
                     /translation="MRSTFTVLEAPPDIFARVLRLQADLAHHHSMWHRRSLGDLFIVE
                     TALFHKAGIIHDDTDYDLIRTVRPTLQLTGV"
     gene            complement(3250..3882)
                     /locus_tag="PLV68_07420"
     CDS             complement(3250..3882)
                     /locus_tag="PLV68_07420"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HRE01114.1"
                     /translation="MATESHRRLLDSLDSLSDEAARTPSLLPGWTVGHVLAHLALNAT
                     SQARVLAAGKSDGEPVAQYPGGREGRPQAIEAASGRPAAVLVADLARAYTAVEQSWVL
                     ADDAAWAAESMTTRGSRPNATLPYRRLRENEIHRVDLGSAGGDYDWTMMPRDFVAITV
                     RELTRQWSSRRPMGMATLPTEVLALPEPTRLAWLTGRLPLPTTPAANLFE"
     gene            complement(3937..4428)
                     /locus_tag="PLV68_07425"
     CDS             complement(3937..4428)
                     /locus_tag="PLV68_07425"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRE01115.1"
                     /translation="MTSSRFSVVDLAPTDTHGLRRAVLRNGDPAAIVTWPGDDVPGVF
                     HLGVRDASGTVVATSSWMPRQHPDLDRDADPETGPPRSIQLRGMATATAVQGSGVGGL
                     LLEAGIARCTDLGFDLLFARARDAALAFYARHGCEVVGDGFVDETTQLPHHLVVRRLR
                     AGG"
     gene            4494..>5060
                     /locus_tag="PLV68_07430"
     CDS             4494..>5060
                     /locus_tag="PLV68_07430"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRE01116.1"
                     /translation="MASAAETMRIESRAASELAALHASGVFWGRGVPRGDGRLVVVVP
                     GLFGNDLYLRPLRDWLRRMGYRPARSSLSINAGCPNRLMDTVMSAIRRQLDESARPVA
                     LVGHSRGGMLCWAMAGALGERVSHLALLGSPAPAVVTMFRNQRDFSPASMANSTVAAA
                     GQRAMQLFDPDCTVPACGCGYVDHIQARL"
BASE COUNT          785 a         1781 c         1710 g          751 t
ORIGIN      
        1 agcgcggcgc cgtcacggtt gagacggtcg atcgagacgg cgccgcagtt cgacgaacac
       61 cggcgtgccg acgatctgct ccaactcctc ggccagcgag tcgatcacgg gcgccgtgcc
      121 gcagtaggcc tcgccgccca gggtgggaaa gaccgggctg atctgctcgg tacagcacca
      181 cgccatcgtc tcgccctcgg cgccccagtc ggcccgcgac cagcgtcgca acgtggccac
      241 ctcgcggtcg acgtcgtgcg gatgctgttc ccagtcgacc ttgatcggga gctgccagca
      301 gaccgacggc ttccagtcga tgggtgactc gtccgcggcg agcgcgccca ggtgcagcgc
      361 gcaccctgca cccccggcga aacccggccg gttcaagaag atgcacgcgc cgtcgaccac
      421 ccgggtgttg acccgctgct cgtcgctgaa caccccgtcg gccgcggcga cgtcgaagaa
      481 ctggaagatc gccggttcga gcgcggcagc cgacgcgtcg accaacatcg cttcgtccac
      541 gccgtcgatc tcggccccca ccgagcagca cccgaggcca cgctcggcct gcggcgtgaa
      601 gtcgatgccg tggcacccgc gaccccagat gcaggtccag ttggaggtca tgaacccggc
      661 ttctattcgc caccgcgtcg atccgtcgtc gatctcgacg tacgcgctct gtgccaccgg
      721 ctcgacgtcc acgaccgaca gcgtacggtc gccatttccg ccgacgacac gccgagggca
      781 ctcagggtcg tcatggagcc ggggtcacca gatcactacc gcgctcgggc ccgcgtgctg
      841 cgcgacctgg agtcgagact gcgcaacgca ggtgtgctga tgctgttcca acgggccggg
      901 accgacgtct ggatcggccc caccccggcc acgtgcctgc aggacctgct cgcgttccgc
      961 aaccgcatct tcnnnnnnnn nnggccgacc aactgcacac gaccgcacgc cacttcgaag
     1021 ccaccgccga acgcctcgct gctgcccacc tcgccgctgg cgctggcgct ggcaccggag
     1081 cgagcggtgc cctgcccagg gtgcgactct catgagcagc tacatgggtt tcgacccgct
     1141 gcgcctgcag agcctggcca gcgcgatgca gcgtgacacc gacgagttgc gccgcctgcg
     1201 atcgagcgaa tccgccgcgg ccgaaccgct gcgagcctgc gctcgcatcg cggacgagtt
     1261 ggagggccgc tggctgcccc tgatacgcaa cctggtcggc tgcggcgtgc tcgacggcta
     1321 ccgccccgcc aatctcgacg acgtctcgct cacccacagc tacctcgact ggctgatccg
     1381 ctcccacggc ttcgtccccg tcaccgaccc cctcgactgg accccatccc ccatgacccc
     1441 cgaccacgct cgcgtcgtcg gagagtggtt ggcgagccgc ggcggcgagg tcagcggtgc
     1501 cgatctcgac gcgttggcgc ggatgctgca tgcgatcgcg acgtcccccg ccgcgtcggc
     1561 cgcgttcgtg cacgggatca acggcacggc cgacctcgat gccacgatcg acgccacgcg
     1621 gctgttcacg ctggtcgtct ccctcagcgg tatcnnnnnn nnnnnnnnnn nnnnnnnggt
     1681 ccaggtggcg gtcaccgcag cgttggccga tcttggccgg atcacggccg ctgtcggcga
     1741 gcacgaagcg atggcaacgg cgctgaccac ggggcgggcc gtcgacccgt actcggccgc
     1801 cctgatcgtc gggcaactcg gtctcaccgc cgagatgctg tcggcgacca ccgccgccct
     1861 gttacgtcgc gaggacgaag agggctgggt cggaggcccg tccgccggag gcgccgtggc
     1921 cgacgcactg tttcccgtca acgtggcgac gccccgcgcg gcgacgctgc tgctccagaa
     1981 cctggcgaac gaggtgtggc tggtgttcac cggcctcgac gtcgcggcca ccgagtcact
     2041 ggtgatgacc ggcatccacg gtgccggtgt cgagggtgcc ggtgtcgagg gtggcagcgg
     2101 cgagggcgct ggcgtcgatg acgcgacccg catcagcctg ctcgctccgc tgctcgacta
     2161 cgtgacggcg cagataccgc tcccctactt ctcccgctcg gacagcccgt ggacggtcga
     2221 gacgctctcc caccttgccg ccgctgcgtt ggccccggac ctggtgatgt tcttctatcc
     2281 aactcccgac cagccacagg ccgacctcgg tctgctcggc cgggtgtacg accagttggc
     2341 agaagcgccg ctgctgttcg acctgctcgt ccgcgagcgg atgcagatgc aagccacgat
     2401 cgactgggcc gatcccgcga gctccaacgc acagaccgag ttgaccctcg tgacgcaact
     2461 cctggacgac atcgcagtca aggccaagat gaccgaagcc gagcgtggcc gggtgtggtg
     2521 gaacctgatg tggtggggga tcggcctgtc cagcaacggc gtccccacgc tgatcggcga
     2581 gatcgtggtt gacgaggtga tcggcctgat ccaagagctc gtcgaagatg ccgggtacgg
     2641 gccgatgact gccgaggagg cccgagacaa ggtgctggtc tcacagaacg gcgacgcgat
     2701 gtggcgcgcg gcgacggtgg ccggcggtct ctatctcgcc gggcgtgacg cgggaacgtt
     2761 cagcgcgtcc gacccggtac cggtccccga ccctgacgcc ggcgacgacg gcgctggcta
     2821 ccgtgcccgg ctcgacgcgt ggttcgaccg tcaccccggc gccagcgcca acatgcgcgc
     2881 cgtggtcgaa aagatctggc ccgtcaaaca acaagccgcc acccacaacc gcctcctcga
     2941 cgccgccaac tgaggacgag ccaacagccg actgccgaac cgaactgtca gaccccggtg
     3001 agttggagcg tggggcggac tgttcggatc aggtcgtagt cggtgtcgtc gtggatgatt
     3061 ccggccttgt ggaacagtgc tgtttcaacg atgaacaagt ctccgagcga gcgacgatgc
     3121 cacatgctgt ggtgatgtgc cagatcggcc tgaagccgca gcacgcgagc gaagatgtcc
     3181 ggcggagctt cgagcacggt gaacgtcgag cgcaacgcct gatcttgggc gtcatagtca
     3241 gaaggatgat cattcgaaga ggttggcggc gggggtggtg ggcagcggga ggcggccggt
     3301 gagccaggcg aggcgggtgg gttcggggag ggcgaggacc tcggtgggca gggtggccat
     3361 gcccatcggg cggcgggacg accactgacg ggtcagctcg cgcacggtga tcgcgacgaa
     3421 gtcgcggggc atcatcgtcc agtcgtagtc gccgcctgcc gagccgaggt cgacgcgatg
     3481 gatctcgttc tcacgcaagc gtcgatacgg cagcgtcgcg ttcggccgcg agccgcgcgt
     3541 ggtcatcgac tcggccgccc acgccgcgtc gtccgcgagt acccaggact gttcgaccgc
     3601 cgtgtacgcc cgagcgagat cggccacgag cacggccgct ggccggccgc tcgccgcttc
     3661 gatcgcctgc ggccgaccct cccgtccgcc tggatactgc gcgaccggct cgccatcgct
     3721 cttgccagca gcgagcaccc gcgcctgact cgtcgcgttc agcgccaggt gggcgagcac
     3781 atggccgacc gtccagcctg gcaacaacga cggcgtccgc gccgcctcgt cgctgagcga
     3841 gtcgagcgag tccaacagac gccgatgcga ctcggttgcc aactcgatcc accgcgtccg
     3901 cgacgccgga ccgaaccgct ccccaacacc catcgctcag cccccggccc gcaatcggcg
     3961 tacgacgagg tggtgtggca gttgcgtcgt ctcgtcgacg aagccgtcgc cgaccacctc
     4021 gcacccgtgg cgggcgtaga aggcgagtgc ggcatcacgc gcccgcgcaa acagcaggtc
     4081 gaacccgagg tcggtacatc gagcaatacc cgcctccaac agcaggcccc caaccccgct
     4141 gccctgcacg gcggttgcgg tcgccatgcc gcgcaactgg atcgatctgg gcgggccggt
     4201 ctcaggatcg gcgtcgcgat cgaggtcagg gtgttggcgc ggcatccacg acgacgtagc
     4261 gaccaccgtc ccgctcgcgt cgcgaacgcc aaggtgaaac acgccaggca cgtcgtctcc
     4321 cggccaggtc acgatcgcgg ccgggtcgcc gttgcgcagc acggcgcggc gcaacccgtg
     4381 ggtgtcggtc ggggcaaggt cgaccacgct gaaccgagag gacgtcatcg gtgccatgat
     4441 ggcggcgctg gccggcgaag ccacgtcggc acgctggggt accgggggta gcgatggcga
     4501 gtgcagcgga gacgatgcgc atcgagagcc gtgccgcgag tgaactcgcc gcgctgcacg
     4561 catcgggggt gttctggggg cgcggcgtgc cacgcggtga cggccggctg gtggtcgtgg
     4621 tgccgggcct gttcggcaac gacctctatc tgcgcccgct gcgcgactgg ctcaggcgaa
     4681 tgggctaccg gccagcgcgc tcgtcactgt cgatcaacgc cgggtgcccc aaccggttga
     4741 tggacacggt catgagcgcc atccgccgcc agctcgacga gtcggcgcgc ccggtggcgc
     4801 tggtgggtca cagtcgcggc ggcatgttgt gctgggcgat ggcgggcgcg ctcggcgagc
     4861 gggtgtcgca cctcgcgctg ctgggttcac ccgcaccggc ggtggtcacg atgttccgca
     4921 accagcgcga cttctcgcca gcgagcatgg ccaactccac cgtcgccgcg gccggtcagc
     4981 gagcgatgca actgttcgac cccgactgca cggtgcccgc gtgcggatgc ggctacgtcg
     5041 accacatcca ggctcggctc
//
LOCUS       DAORNF010000153         5046 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_12325_length_5046_cov_1.927469, whole genome shotgun sequence.
ACCESSION   DAORNF010000153 DAORNF010000000
VERSION     DAORNF010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 5046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5046
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12325_length_5046_cov_1.927469"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>264
                     /locus_tag="PLV68_07435"
     CDS             <1..>264
                     /locus_tag="PLV68_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01117.1"
                     /translation="VFLAGLAAVTTWREDGRQVLVVALPLAGLVMASIYHSVGGRSHE
                     GTNLLLDEIHEPTAWVPLRMAPLVLVGTWMSHLFGASVGREGTS"
     assembly_gap    265..388
                     /estimated_length=124
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <389..1158
                     /locus_tag="PLV68_07440"
     CDS             <389..1158
                     /locus_tag="PLV68_07440"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HRE01118.1"
                     /translation="AVFGLEVQVLGRRRLTAVVPALTASFVGNGVMHALRGHHTEYAP
                     IRPDLSGSLIAGLVVAGVAFGIVGASYPVLTHALKRRMARWFVWAPWRPFVGGLATIA
                     LVLVFGHAYQGLSLPFLADAMSGAGAVGVSVFALKLLFTVVAVGSGFFGGEVTPLFVV
                     GATLGSALATPVGAPPAVFAAAGLCAVFGAAANAPLTCIVMGVELFGWHMIVPLMITC
                     LAAYAVSAPEGVYRSRRAHLAKVAVNLRKLRQGSRRA"
     gene            1168..1599
                     /locus_tag="PLV68_07445"
     CDS             1168..1599
                     /locus_tag="PLV68_07445"
                     /inference="COORDINATES: protein motif:HMM:NF024055.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HRE01119.1"
                     /translation="MKAALLVESLTGNTWKAAELVATELQGAGWDITGLSKVRQPDHG
                     SIQAADLVLVGTWTHGLFVVGQGPWGAAALSQLPAMRGKKAAVFCTFALNPAKTLDKM
                     TSIITGVGADVVGGLAMNRRHLERNAHEFAGRLLDAAPALV"
     gene            1889..2809
                     /locus_tag="PLV68_07450"
     CDS             1889..2809
                     /locus_tag="PLV68_07450"
                     /inference="COORDINATES: protein motif:HMM:NF013341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIO1 family regulatory kinase/ATPase"
                     /protein_id="HRE01120.1"
                     /translation="MSKRFRRLDDDDLLVPQSTFHDQPTDGMLDDARRDDVSGDDGDD
                     GDDDDQPDGGPVQSTYPDADHGPQPVPQWVITHADALDDDLGVLKSGKEADVSLLRRS
                     HGAATSLMAVKRYRSAEHRLFHRDAGYLEGRRVRRSRENRAMERRTEFGRELIAGQWA
                     RAEFSFLTLLWSRGAPVPYPVQLCGTELMMEFIGSADGVAAPRLAALRPSRAEAEELF
                     VQMCDALGVMAEAGYVHGDLSPYNVLVDQDSGRLLLIDLPQAVDLVGNPQGFAFLRRD
                     CDNICSWFTARGVDASPAALEADLLRRAGA"
     gene            complement(2835..>3338)
                     /locus_tag="PLV68_07455"
     CDS             complement(2835..>3338)
                     /locus_tag="PLV68_07455"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HRE01121.1"
                     /translation="GCQAVVVPYSGYEMSMVIVLPDTSSGADAALAARLPEIAAATSS
                     RKVELSLPRWDFGSRAQLADVLAALGMPSAFDPSRADLSAMSQSDEAPLYISKVIHEA
                     NITVDEDGTEAAAATAVLVVPTSAPLPTEPIPFVCDRPFLFAVRDDATGALLFTGRVA
                     DPTQTRD"
     assembly_gap    3339..3411
                     /estimated_length=73
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3412..>3890)
                     /locus_tag="PLV68_07460"
     CDS             complement(<3412..>3890)
                     /locus_tag="PLV68_07460"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HRE01122.1"
                     /translation="FSPASIAIALAMVRAGAAGATATEMDTVLHVTDPAALAPSMNGL
                     QLALDARTHAANSTLGREQVTLSIGNSLWGQVGYRFEQTFLDTLAANYDAGMQLVDYK
                     ADAEGARQMINAWVAEQTRDRIAQLLAPDILSPDVRLTLVNTIYLKAPWGSQFNRDL"
     assembly_gap    3891..3949
                     /estimated_length=59
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3950..4165)
                     /locus_tag="PLV68_07465"
     CDS             complement(<3950..4165)
                     /locus_tag="PLV68_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01123.1"
                     /translation="MATDAPPLLPALDRRRFVGLLATPVLASLLTALQACGSDTPATG
                     NTANTGDNSDGTSVGLLRSDASRARAGD"
     assembly_gap    4223..4241
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4242..4692)
                     /locus_tag="PLV68_07470"
     CDS             complement(<4242..4692)
                     /locus_tag="PLV68_07470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01124.1"
                     /translation="MRRIELRSPFARAVVPVLAGIGFFVVLGLVLWAIAAGISGERSD
                     STSNLAPSTADIGHTQTYARIIADAGPIILPDLLEASGRRTIVLDHTGDDDKAGWRIY
                     MAYPADRSVDCKVTQIERTRDFTDCDGRTIATEDLAKPPVGVRPIVSA"
BASE COUNT          680 a         1543 c         1724 g          824 t
ORIGIN      
        1 ggtgttcctg gcggggctgg ccgcggtgac cacgtggcgt gaggacggtc gccaggtgct
       61 ggtggtcgcg ctgccgctgg ccggactggt gatggcgtcg atctatcaca gcgtcggcgg
      121 ccgctcgcac gagggcacca acctgctgct cgacgagatc cacgagccga ccgcgtgggt
      181 gccgctgcgg atggctccgc tggtgctggt cggcacgtgg atgtcgcatc tgttcggcgc
      241 atcggtcgga cgcgagggga cctcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnngg gcggtgttcg ggttggaggt gcaggtgctc
      421 ggccgccgcc ggctgacggc tgtagtgccc gcgttgaccg cgtcgttcgt cggcaacggc
      481 gtcatgcacg ccctccgtgg ccaccacacc gagtacgcgc cgatccggcc cgatctgtcg
      541 ggcagcctga tcgctggtct ggtcgtggcc ggggtggcgt tcggcatcgt cggcgcgtcg
      601 tacccggtgc tgacccacgc cctcaagcgg cggatggcgc ggtggttcgt gtgggcgccg
      661 tggcgaccct tcgtcggggg cctcgcgacc atcgccctgg tcctcgtgtt cggccatgcc
      721 taccaggggc tgtcgttgcc gttcctggcc gatgcgatga gcggtgctgg tgcggtgggc
      781 gtgtcggtgt tcgcgttgaa gctgctgttc accgtggtcg cggtcggcag cgggttcttc
      841 gggggcgagg tcacaccgtt gttcgtcgtc ggagccacgc tcggttcggc gttggccacg
      901 ccggtggggg caccgcccgc ggtgttcgcc gcggctggct tgtgcgccgt gttcggcgcc
      961 gcggccaacg cgccgctgac ctgcatcgtc atgggcgtcg agctgttcgg gtggcacatg
     1021 atcgtgccgc tgatgatcac ctgcctcgcc gcgtatgcgg tcagcgcccc ggaaggggtc
     1081 taccgatcgc gtcgcgccca tctcgcgaag gtggcagtca acctccgaaa gctccgtcag
     1141 ggttcacggc gtgcttgaat caggggcgtg aaagcagcgc ttcttgtcga gagtctcacc
     1201 ggcaacacgt ggaaggcggc cgaactggtg gccaccgaac tgcagggggc agggtgggac
     1261 atcaccggcc tgtccaaggt gcgtcagccc gatcacggca gcatccaggc tgccgatctg
     1321 gtgctggtgg gcacgtggac ccacggtctg ttcgtcgtcg gccaagggcc gtggggtgcg
     1381 gccgcgctca gccaactgcc ggcgatgcgg ggcaagaagg ccgcggtgtt ctgcacgttc
     1441 gcgctcaacc cggccaagac actcgacaag atgacatcca tcatcaccgg tgtcggcgcc
     1501 gacgtcgtcg gcgggttggc gatgaaccgc cgtcatctcg aacgcaacgc ccacgagttc
     1561 gccggacgct tgctcgacgc cgcccccgcc ctggtctgaa cgggctgggc cgacgccgct
     1621 cggccctgtt cggccctgac cagaccgttg ctggagcgcg gtgatattcg gggttcgtcg
     1681 ccgcgtgacc atgcactagg ttgacgcacg ttgccacgga atgggccggg cacgcagacg
     1741 cgaggaggtg ctgccgtgat tgccatcatc gccttccgcc gccgcgcctg tcgcgcggct
     1801 gtccgttcct gctcgtaacg gcaccgcccc tcaaccctcg ctctgacgcg acctccggga
     1861 ggtgcctccc gcaccccgag gtgttctcgt gtccaagcgt ttccgtcgtc tcgatgacga
     1921 cgaccttctc gttccccagt ccacgttcca cgaccaaccg accgacggca tgctcgatga
     1981 cgcccgtcgc gatgacgttt ccggcgatga cggcgatgac ggcgatgatg acgaccagcc
     2041 cgatggcggg ccggtgcagt cgacctatcc cgatgccgat cacggcccgc agcccgtgcc
     2101 gcagtgggtg atcacccatg ccgacgccct cgacgacgat ctcggtgtgc tcaagtcggg
     2161 caaggaggcc gacgtgtcgt tgctgcgccg cagccacggc gcggcgacca gcttgatggc
     2221 ggtcaagcgc tatcgcagcg ccgagcaccg cctgtttcac cgcgacgccg gctacctcga
     2281 aggccgccgc gtgcgccgca gccgcgagaa ccgggcgatg gagcgacgta ccgagttcgg
     2341 ccgtgagctg atcgccggac agtgggcgcg agcggagttc tcgttcctga cgttgctgtg
     2401 gtcgcgcggc gcgccggtgc cgtatcccgt gcagctctgc ggcaccgagc tgatgatgga
     2461 gttcatcggc agcgccgacg gcgtcgccgc gccccgactg gctgcgctgc gtccgtcgcg
     2521 ggccgaagcc gaggagttgt tcgtccagat gtgtgacgcg ctcggggtga tggccgaggc
     2581 agggtacgtc cacggcgacc tgtcgccgta caacgtgttg gtcgatcagg acagcggccg
     2641 attgctgctg atcgatctgc cgcaagcggt cgacctggtc ggcaacccgc aaggcttcgc
     2701 cttcctccgt cgtgactgcg acaacatctg tagctggttc acggcgcgcg gcgtcgacgc
     2761 ctcgccagcc gcgctggaag ccgatctgct ccgccgcgcc ggtgcgtgac ctgacagcgg
     2821 tagtggacgc gacctcagtc gcgggtctga gtggggtcgg cgacgcggcc ggtgaacagc
     2881 agcgcgccgg tcgcgtcgtc gcgcaccgca aacaggaacg gccggtcgca gacgaacggg
     2941 atcggttcag tcggcagcgg cgccgatgtg ggcaccacga gcaccgcggt cgcagcggca
     3001 gcctcggtgc cgtcctcgtc gacggtgatg ttggcctcgt gaatcacctt ggagatgtac
     3061 agcggtgctt cgtccgactg ggacatcgcc gagaggtcgg ctcgactggg atcgaacgcc
     3121 gagggcatgc cgagcgcggc gaggacgtcg gccaactgcg cccggctgcc gaagtcccac
     3181 cgcggcaatg acagctccac cttgcgcgac gacgttgccg cggcgatctc gggtaaccgg
     3241 gccgcgagcg cggcgtcggc gccgcttgat gtgtcgggca gcacgatcac catgctcatc
     3301 tcgtacccgc tgtagggcac cacgacggcc tggcatccnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngagatcacg
     3421 gttgaactga cttccccacg gcgccttgag gtagatggtg ttgaccaacg tcaggcgtac
     3481 gtccgggctc aggatgtcgg gagccagcag ttgcgcgatg cggtcgcggg tctgctcggc
     3541 gacccaggcg ttgatcatct gacgagcgcc ttctgcatct gccttgtagt cgaccagttg
     3601 catgccggcg tcgtagttgg cagcgagtgt gtcgagaaac gtctgttcga atcggtatcc
     3661 cacttggccc cacaacgagt tgccgatcga cagcgtgacc tgttcgcgac cgagggtgct
     3721 gttggcagcg tgggtgcgcg catcgagcgc gagttgcagg ccgttcatcg acggggcgag
     3781 tgctgccgga tcggtgacgt gcaacacggt gtccatctcg gtcgcggtcg caccggctgc
     3841 tccggcgcgc accatcgcca gtgcgatcgc gatgctggcg ggcgagaaca nnnnnnnnnn
     3901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng tcgcccgcgc
     3961 gagcgcggct cgcgtcggaa cgcagcaggc cgaccgacgt gccgtcggag ttgtcgccag
     4021 tgttcgccgt gttgccggtg gctggcgtgt ccgagccgca ggcttgcagc gcggtgagca
     4081 gcgacgcgag cactggcgtg gccagcaggc cgacgaagcg gcggcgatca agggcgggga
     4141 gcagcggggg agcgtcggtg gccatgccta ctctgacgat gcggtggtcg gctgtggttc
     4201 cgccgcgtcg ggcagcagat cgnnnnnnnn nnnnnnnnnn ncggcggaca cgatcgggcg
     4261 cacaccgaca ggcggcttgg ccagatcctc ggtggcgatg gtgcgtccgt cgcagtcggt
     4321 gaagtcgcgt gtccgttcga tctgggtgac cttgcagtcc acgctgcgat ccgctggata
     4381 ggccatgtag atgcgccatc cggccttgtc gtcgtcgccg gtgtggtcga gcacgatggt
     4441 gcgccgcccg ctcgcttcga gcaggtcggg caagatgatg gggccggcgt cggcgatgat
     4501 ccgggcgtag gtctgggtgt ggccgatgtc ggcagtcgac ggcgcgaggt tgctggtcga
     4561 gtccgaccgc tcgccggaga tgccagccgc gatcgcccac aacaccagac cgagcacgac
     4621 gaagaagccg atgccggcca gcaccggcac gaccgcacgg gcgaaggggg agcggagttc
     4681 gatccggcgc atcgctgcca gtgtgcccga gcctgcccgc cccacgcaca tcagcacccg
     4741 atcgcgggtg gaccgagtgg tggcgtgggt gcctgagtgg gtacatgggt ggtgcatggg
     4801 tggtcgcgcg aacagccctg gtttgggcga ccatctccag cgttgcgtag agttccgtac
     4861 tcgtgagcca ccagcgtcga gcagggcgag cattcggaca acgagaggat tgaccccgtg
     4921 gatctcttcg aatatcaggg caagcagtac tttgcccgct atgacatccc ggtcagcccg
     4981 ggcggcgtag cagacaccgt cgatcaggcg gtcgcgcagg ccgacgcagc cggctacccc
     5041 gtggtg
//
LOCUS       DAORNF010000154         5044 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_12332_length_5044_cov_1.666065, whole genome shotgun sequence.
ACCESSION   DAORNF010000154 DAORNF010000000
VERSION     DAORNF010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 5044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5044
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12332_length_5044_cov_1.666065"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>229)
                     /locus_tag="PLV68_07475"
     CDS             complement(<1..>229)
                     /locus_tag="PLV68_07475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01125.1"
                     /translation="ISTNAAGAKSLDAHDLASWLRSEFNLLATTDGTPPGSAAAVQKA
                     VDALSFAPMVTPLHNVIDQAHWVGRSDWPGNA"
     assembly_gap    230..387
                     /estimated_length=158
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<388..>817)
                     /locus_tag="PLV68_07480"
     CDS             complement(<388..>817)
                     /locus_tag="PLV68_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01126.1"
                     /translation="AQYEQDNFALDPALGGARVTYLGNNYLAIPFYAQAVTYAFSVPT
                     FVAQGALILHPVTGGQLIGDPDLTAAGGRYSDLIDPAEEPQKSMIATINAQVALVASI
                     ANAFSEEMLVEATKQFYLKADVDGEIEAAIDNFAESVWLAA"
     assembly_gap    818..859
                     /estimated_length=42
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<860..1217)
                     /locus_tag="PLV68_07485"
     CDS             complement(<860..1217)
                     /locus_tag="PLV68_07485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01127.1"
                     /translation="MDYVVAPLIDDSAERVIVTGNGATPEAWQGGVYRQLGVPAPTAA
                     PTVTVNVVDEFSIEDAQTFKAGALKQYEDAILAAVSTAYAGYANVVSSGARGSVWLPH
                     GTVAGMPTSDSRQGCYC"
     gene            complement(1459..>2045)
                     /locus_tag="PLV68_07490"
     CDS             complement(1459..>2045)
                     /locus_tag="PLV68_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01128.1"
                     /translation="QKVMLLHRPDLFSVLATRALSAGARQTIPADGSKLLEVVRNTSG
                     GRPITLVKDRRLLDVVSPTWYSMTGVTEIKHYMHDERDADAFWVYPPAASSGASVDLL
                     YVQNPTDVAVPADGTTVDDVMGDLSVPDFIAPAVDEWVLFRAYLQDSEAASPNAQRAM
                     VHARSFADLLGVELNGLLASAPRSAGRAMSPAAQ"
     assembly_gap    2046..2061
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2150..>2429)
                     /locus_tag="PLV68_07495"
     CDS             complement(2150..>2429)
                     /locus_tag="PLV68_07495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01129.1"
                     /translation="VLNGPAGTLQVLAALEPTATATTLPDFLARRYLEAIAAGAAARL
                     LLTSKASFFDPAAAGLHRARFDEAVGAAGVAVMRGNTNTTPRHAIRWC"
     assembly_gap    2430..2460
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2461..2758)
                     /locus_tag="PLV68_07500"
     CDS             complement(<2461..2758)
                     /locus_tag="PLV68_07500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01130.1"
                     /translation="MAALDVFLPDVLAHAPDAPDPYARLCLVRAAQEFCQRTFAWKAW
                     LACVAGTAPNYTATALADSQVVRLEAATLNGTPTELGQWWTAERDPATYATTESA"
     gene            complement(2765..>3061)
                     /locus_tag="PLV68_07505"
     CDS             complement(2765..>3061)
                     /locus_tag="PLV68_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01131.1"
                     /translation="VTVQGEDGATYVFRTAGKTGPLTCDVPDEATVAKLLRLETFIPV
                     NEADFDVATKLVGGDDDDDLDPDGDHDDAADQNAPPEEANTPPRPRRKRAQQVT"
     assembly_gap    3062..3094
                     /estimated_length=33
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3196..>3384)
                     /locus_tag="PLV68_07510"
     CDS             complement(3196..>3384)
                     /locus_tag="PLV68_07510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01132.1"
                     /translation="GAHWGVTTAVNTAFLQRLTPNVNAMATVAKSDSPRKLGVKVTTA
                     PTTPVAGTLGVTLLYRAA"
     assembly_gap    3385..3476
                     /estimated_length=92
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3843..3909
                     /estimated_length=67
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3910..4800)
                     /locus_tag="PLV68_07515"
     CDS             complement(<3910..4800)
                     /locus_tag="PLV68_07515"
                     /inference="COORDINATES: protein motif:HMM:NF024649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N4-gp56 family major capsid protein"
                     /protein_id="HRE01133.1"
                     /translation="MARTIVGVNDAKAVKRFTGLLAMDASQRGWWSNRFMARGAEAEV
                     PIQILTDLESDAGEEIKYDLLAELRMAPVEGEDNLEGNEEAQRFYTDSVYIDQARCGV
                     NTGGRMTRKRTLHNLREKARRQQSNWWARMQDEMLFMYQAGARGVNDNFLFPSNWAGR
                     ANNALVAPDANHIVYGGDATSFANIDATDKLTLPAITAALMKALTQGGGATNIPVLQP
                     CKIDGAEVFVGVMHTWQEDDLRKSTVTGDWLDLQKAAAGAEGKNSPLFRQSLGMHRNI
                     VLHSHRNVIRFSNAGSGANVA"
BASE COUNT          711 a         1520 c         1575 g          799 t
ORIGIN      
        1 gcgttgccag gccaatccga gcggccgacc caatgcgcct gatcgatcac gttgtgcagc
       61 ggggtgacca tgggcgcgaa cgacagcgca tcgacggcct tttgcaccgc ggccgcagac
      121 cctgggggcg tcccgtctgt cgtcgccagc aggttgaact ccgagcgcag ccagctcgcc
      181 aggtcgtgtg cgtccagcga cttggccccg gccgcgtttg tcgagatgcn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnncgc cgccagccac acggattcgg cgaagttgtc
      421 gattgccgcc tctatctcgc cgtcgacatc agccttgagg tagaactgct tcgtcgcctc
      481 taccaacatc tcctccgaga atgcgtttgc gatcgacgcg accagcgcta cctgagcgtt
      541 gatcgtggcg atcatcgact tctgcggctc ctcggctggg tcgatcaggt ccgagtaccg
      601 gcccccggcc gccgtcaggt ctggatcccc gatgagttga ccaccggtga cggggtgcag
      661 gatcagcgca ccctgggcga caaacgtcgg caccgagaat gcgtaggtca ccgcctgggc
      721 gtagaagggg atcgccaagt agttgttgcc gaggtaggtg acgcgggcgc caccgagcgc
      781 ggggtctagc gcgaagttgt cctgctcata ctgcgccnnn nnnnnnnnnn nnnnnnnnnn
      841 nnnnnnnnnn nnnnnnnnng gcagtagcac ccctgccggc tgtcagacgt cggcatgcct
      901 gcgacagtcc catgcggcag ccacacagac ccgcgcgcgc cgctcgacac gacgttggcg
      961 tagccggcgt aggctgtaga caccgccgcc aggatcgcgt cctcgtactg cttgagcgcg
     1021 ccggccttga acgtctgcgc atcctcgatc gagaactcgt cgacgacatt cacggtcacc
     1081 gtcggcgccg cggtcggcgc cggaacgccg agttgacgat agacgccacc ttgccaggcc
     1141 tccggtgtcg cgccattgcc ggtcacgatg acccgctcgg ccgagtcgtc gatcagcggc
     1201 gcgacgacgt agtccacgtc tgacgcagat gacagccacg tcccagtgat cggcgtcttg
     1261 aacaccgact tggccgcaga ggcgacggtc gacacactcg cccccgggcc cagtgctggg
     1321 cggaagtcgc cgcgccggag atccaggttt tccgcggccg ccgccgagcc gaagggcagc
     1381 cgtcgatcgt cgaccgacgg gagaatcccg ccgaactcga tcaggtccag ggacgccacg
     1441 cgaatgcccc caacggcctc attgcgccgc tggcgacatc gcgcgcccgg cgcttcgtgg
     1501 cgccgacgcc aacaggccat tcagttcgac gcccagcagg tcggcgaacg atcgcgcatg
     1561 caccatggcg cgctgcgcgt tgggggatgc ggcctcgctg tcctgcaggt aggcccggaa
     1621 cagcacccac tcgtcgacgg ccggcgcgat gaagtctggc accgacaggt cgcccatgac
     1681 atcgtcgacg gttgtgccat cggcaggcac ggccacatcg gtcgggttct ggacgtagag
     1741 caggtccacc gatgcgccgc tgctggccgc aggggggtag acccagaagg cgtccgcgtc
     1801 gcgctcgtcg tgcatgtagt gcttgatctc ggtgacaccg gtcatcgagt accaggtcgg
     1861 cgacacgaca tcgagcagcc gacggtcctt gaccagggtg atcggacggc cgccactggt
     1921 gttgcgcacg acctccagca gcttcgagcc gtcggctggg atcgtctgcc gagcgccggc
     1981 cgacagagct cgcgtggcga gtacagagaa caggtccggg cggtgcaaca gcatcacctt
     2041 ctgcgnnnnn nnnnnnnnnn ncagagacgg gatggaccag aacgtcgagt cggcgtcgac
     2101 caggatttcg atcacgcgct cgattcgtga ttttgcggtc gtggccatgt cagcaccacc
     2161 ggatcgcgtg gcgcggcgtc gtgttggtgt tgccgcgcat cacggccacg ccagcggcgc
     2221 cgacggcctc gtcgaagcgg gcccgatgca ggccggcggc ggcagggtca aaaaacgagg
     2281 ccttcgaggt cagcaacagc ctcgccgcgg cgccggcggc gatggcttcg aggtatcgcc
     2341 gcgccagaaa gtcgggcagc gtggtcgccg tggccgtggg ctccagcgcg gccaggacct
     2401 gcagcgtgcc ggccgggccg ttcaggacgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 cagccgattc ggtggtggcg taggtggccg gatcgcgctc ggcggtccac cactggccca
     2521 gttcggtcgg cgtgccgttg agcgtggcgg cctccaggcg cacgacctgc gagtcggcca
     2581 gcgccgttgc ggtgtagttc ggcgccgtgc cggcgacgca tgccagccag gccttccagg
     2641 cgaacgtgcg ctggcagaac tcctgcgcgg cccgcaccag gcacagcctg gcgtaggggt
     2701 ccggtgcgtc aggggcgtgc gccagcacgt ccggcaggaa gacatccagc gcggccacgg
     2761 gcgatcacgt cacttgctgg gcgcgcttgc gcctcggccg cggcggcgtg ttcgcctcct
     2821 ccggcggggc gttctggtcg gcggcgtcgt cgtggtcacc gtccgggtcg aggtcgtcgt
     2881 cgtcgtcgcc accaaccagc ttggttgcga cgtcgaaatc ggcctcgttg acagggatga
     2941 acgtctccaa gcgcaacagc tttgcgacgg tcgcctcgtc gggcacgtcg caggtcaggg
     3001 gcccggtctt gcccgccgtg cggaagacgt aggtagcgcc gtcttcgcct tgaacggtga
     3061 cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnggtggc ctcgagtagc atgggttggg
     3121 actcctgtag aaaaaagggg ggccgaagcc ccccgctcaa tgaccctcag gcggcgcgga
     3181 gagtgccgcg gtggatcagg cagcccggta cagcagcgtc acgccgagcg tgccggcgac
     3241 aggcgtggtc ggggcggtgg tcaccttgac gccgagcttg cgaggtgagt cggacttggc
     3301 gaccgtggcc atcgcgttga cgttcggcgt caggcgctgc aggaacgcgg tgttgacggc
     3361 ggtcgtcacg ccccaatgcg cgccnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnggac
     3481 aatgtggcga cgagtcgaat cgtgggaatg ctcgccgatc tggccgatcg tgttcaacgc
     3541 aaggtcgccg gtggccatgg acaacgtgaa gcgggtcgcc agcacttcgg cgccggccgg
     3601 ggtcggcacc ggcttgcggc cggtgatgta atcgttcgaa gccgagaaag ccatgttgtg
     3661 ctcctgtgat ggtcagcgcg gatcagcgga cggcgcaggc cgtgtcgacg gcgatcgttc
     3721 cgaagtcctg cgggccggct tcggtcgtga acgtcgtctt cttcgcaccc aagatcgcgc
     3781 tggtcgagat cacgaccttg tcgccgttgt cccgggtctc ctcgtaccag tcataccgca
     3841 tgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnnnnng gccacgttgg cgcctgagcc agcgttcgag aaccgaatga cgttgcgatg
     3961 cgagtgcagc acgatgttcc tgtgcatgcc aaggctctgc ctgaacaacg gcgagttctt
     4021 gccctccgca ccggctgcgg ccttctgcag gtccagccag tccccggtca ctgtgctctt
     4081 gcgcagatcg tcctcctgcc aggtgtgcat cacgcccacg aacacctcgg cgccgtcgat
     4141 cttgcatggc tgcagcaccg ggatgttcgt cgcgccgccg ccttgcgtga gcgccttcat
     4201 cagcgccgcg gtgatcgccg gcaacgtgag cttgtcggtg gcgtcgatgt tggcgaacga
     4261 ggtcgcgtcg ccgccgtaga cgatgtggtt cgcgtccggc gcgaccagtg cgttgttcgc
     4321 gcgaccggcc cagttggagg ggaacaagaa gttgtcgttg acgccgcgcg cgcccgcctg
     4381 gtacatgaac agcatctcat cctgcatgcg ggcccaccag ttggactgct ggcggcgggc
     4441 cttctcacgc aggttgtgga gcgtgcgctt gcgggtcatg cgcccgccgg tgttcacgcc
     4501 gcagcgggcc tggtcgatgt agacagagtc ggtgtagaag cgctgtgctt cctcgtttcc
     4561 ttcgaggttg tcctcccctt cgaccggcgc catgcgcagc tcggccagca ggtcgtactt
     4621 gatctcctcg ccagcatccg actcgaggtc ggtcaggatc tggatcggca cctcggcttc
     4681 ggcgccgcgc gccatgaacc ggttggacca ccagccgcgt tgactggcgt ccatcgccag
     4741 caggcctgtg aatcgcttga ctgccttggc gtcattgacg ccgacgatgg tgcgggccat
     4801 tgatgggctc ctcttgggtt gaaccagagg agcacgtcat gcgctccgat cgacggcctt
     4861 ggccgttcag gtcgctacta tgcgcgtgct tgtatcgact tcagacgtct cgtcgcactg
     4921 caatcgcacg cggacctcgc gcggcgccat cacacgaatt cgggcgacct gcccgctttt
     4981 gcgcagcacc tcgatggaga tgccgcccag gtcaagccac tgacccggtc tgacgtcgag
     5041 cacg
//
LOCUS       DAORNF010000155         5039 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_12350_length_5039_cov_1.525482, whole genome shotgun sequence.
ACCESSION   DAORNF010000155 DAORNF010000000
VERSION     DAORNF010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 5039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5039
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12350_length_5039_cov_1.525482"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>703
                     /locus_tag="PLV68_07520"
     CDS             <1..>703
                     /locus_tag="PLV68_07520"
                     /inference="COORDINATES: protein motif:HMM:TIGR01726.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRE01134.1"
                     /translation="KELLFRSGSVRSVVGKANESGLLLFPPTGIPAVLHSLKARRLER
                     QGEIEQARVSGERARDWAWYSVGVGLLVYVVAFLVYLLTVKNGAVRKVNFDWNFLWNR
                     AIWSSVWRGFRLNLKVFAVASLLVMVWGLIVAVVRLLPGKQCAPIRFVATLYCDVFRA
                     IPALLVIGIVGFGIPKANPPILGGRSDFFYATLSLVLVYGAYVSEVYRAGITSIHWSQ
                     TAAARSLGLSYRQTMT"
     assembly_gap    704..828
                     /estimated_length=125
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            963..1700
                     /locus_tag="PLV68_07525"
     CDS             963..1700
                     /locus_tag="PLV68_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016078581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HRE01135.1"
                     /translation="MLELFDVHKYFGENHVLRGVNLDVDSHEVVCLIGASGCGKSTML
                     RCINALEQIDGGSIWLHGDRISGAGIDVDSLRTQVGIVFQSYNLFPHMTVRRNITLAP
                     RKVLGMDAADADARAIDLLERIGLADKADEYPDRLSGGQQQRVAIVRALAMNPQLLLL
                     DEITSALDPELVAEVLNIVRALAADGMTIVMATHEMGFAREVASKVCFVHEGVIHEEG
                     SPVQIFSEPQHERTQAFLKRIIDAGRL"
     gene            1820..>2296
                     /locus_tag="PLV68_07530"
     CDS             1820..>2296
                     /locus_tag="PLV68_07530"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRE01136.1"
                     /translation="MGVNDRILDATKVCCERYGIAKVSIDDIAAEAGVTRATLYRLFP
                     GGKDVVFDEFFNRIQTALVGADTLEDLLVGAVVAATNDLRNDHHLAIMLMSEPGETLA
                     NLTVTGMPRIIRMATLFLSPLVGAHLDRPHATRVVDVLARLVISYFLAPSDDVDLGD"
     assembly_gap    2297..2409
                     /estimated_length=113
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2450..>3485
                     /locus_tag="PLV68_07535"
     CDS             2450..>3485
                     /locus_tag="PLV68_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HRE01137.1"
                     /translation="MHNDNETILGRGEIDDIEAILAIPVVDPAEVEHIVKNNADSIFT
                     WDYSLARPALRKLYEKAKSGQWNGTVDLDWKTDVDVEKSVAEDQAVLGTGFDRNTYIG
                     TPVEKWGDKEWLEFGIEGRNWQLSQFLQGEKGELLCTAKIVETVPWYDAKLYASTQVM
                     DEARHVEVFARYLDEKLGGGYQINAHLRLLLDDIINDSRWDMTYLGMQVMVEGLALAA
                     FGFLHSITQEPLLKKLLRYVMSDEARHVAFGVLSLKEVYDGMTDTEIKDRQEFAFEAA
                     VRMRDRFMSQEVWERMGIDMHDIMPLLLQDPTRGTFQEMLFSKIVPNCKKLGLLDRND
                     KWLRHRFEELG"
     assembly_gap    3486..3626
                     /estimated_length=141
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4018..4086
                     /estimated_length=69
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4087..4881
                     /locus_tag="PLV68_07540"
     CDS             <4087..4881
                     /locus_tag="PLV68_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HRE01138.1"
                     /translation="LATAALACIAPDSSRTATSIHSAVAEAVTIPVVAGTGTNDTPHS
                     IHMTREATALGAAGVLAVCPYYNRPSQAGIEAHLRAMAAATDLPLVVYDIPVRTGRKI
                     ATSTLLRLFREVPNVVALKDAAGNPAETASLIAAAPNGVEVYSGDDAMTLPLLSVGAV
                     GVIGVATHWSGDDHQQMLDLWEKGDVVGARIVNSRLLESFAYETGDEAPNPIPTKAMM
                     RHLGIPVGQARLPMGDAPDFVVERAPHVWNNLARWRDAFPLCPADA"
     gene            4850..>5039
                     /locus_tag="PLV68_07545"
     CDS             4850..>5039
                     /locus_tag="PLV68_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01139.1"
                     /translation="MPSPSALPMPDHISIVFLGGLGEIGRNCMAIDQQGQVLLIDCGL
                     MFPDADMHGIDLVLPDFTW"
BASE COUNT          766 a         1529 c         1500 g          796 t
ORIGIN      
        1 aaggagctgc tgttccgctc gggaagcgtg cgctcggtcg tcggcaaggc gaatgagagc
       61 ggtttgctgc tgttcccgcc gaccggcatc cccgccgtgc tccactcgct gaaggcccgg
      121 cgactcgagc gccagggcga gatcgaacag gcccgagtgt ccggcgagcg ggcccgcgac
      181 tgggcgtggt actcggtcgg cgtcggcctg ctggtctacg tcgtcgcctt cctcgtctac
      241 ctgctgaccg tcaagaacgg ggcggtgcgc aaggtcaact tcgattggaa cttcctgtgg
      301 aaccgcgcga tctggtcgtc ggtctggaga ggcttccggc tcaacctcaa ggtgttcgcg
      361 gtcgcgtcgt tgctggtgat ggtgtggggg ctgatcgtcg cggtcgtgcg gttgctgccc
      421 ggcaaacagt gcgcgccgat ccgtttcgtg gccacgctgt attgcgacgt gttccgcgcc
      481 atcccggcgt tgctggtgat cggcatcgtc ggcttcggca tccccaaggc caacccgccg
      541 atcctcggcg ggcggagtga cttcttctac gccaccctgt cgctggtgct ggtctacggc
      601 gcgtacgtgt ccgaggtcta tcgggcgggc atcacgtcga tccactggag tcagaccgcc
      661 gcagcccgct cgttggggct cagctaccgc cagacgatga cgtnnnnnnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnaa ggacccgccg
      841 gtgtgggcgt tcccgttctt cgccgcgatc ctcttcgcgc tgatcagtct gccgctcacc
      901 cgcctgctcg actggatgac ccaccgcgag tacgccaaga cctcgggtgg ttcgtgatgg
      961 ccatgctgga actgttcgac gtgcacaagt acttcggcga gaaccacgtg ctgcgtgggg
     1021 tcaacctcga tgtcgacagc cacgaggtgg tctgtctcat cggtgcgtcg ggctgcggca
     1081 agagcacgat gctgcgctgc atcaacgcgc tcgagcagat cgacgggggt tccatctggc
     1141 tccacggcga ccgcatcagc ggcgcgggca tcgacgtcga ctcgctgcgg actcaagtcg
     1201 gcatcgtgtt ccagagctac aacctgttcc cgcacatgac ggtgcggcgc aacatcaccc
     1261 tggcgccgcg caaggtgctc ggcatggatg cggccgatgc cgacgcgcgg gcgatcgatc
     1321 tgctcgagcg gatcggcctt gccgacaagg ccgacgagta ccccgatcgc ctctccggcg
     1381 gccagcagca gcgggtcgcg atcgtgcgcg cgctggcgat gaacccccag ttgttgctgt
     1441 tggacgagat cacgtccgcg ctcgacccgg aactggtggc tgaggtgctc aacatcgtcc
     1501 gcgcgttggc ggccgacggg atgacgatcg tgatggccac ccacgagatg ggttttgctc
     1561 gcgaggtcgc gtccaaggtg tgcttcgtgc acgaaggcgt gatccacgaa gaggggtcgc
     1621 cggtgcagat cttctccgaa ccccagcacg agcgcaccca agccttcctg aagcggatca
     1681 tcgacgccgg tcggctctga ttcctgcggc gagagaggca ccggcgacga gtcgccgtga
     1741 gggggcggcc acaggcgccg ggcgtcagtc ccgaacagtg ggttgacact gagacagatt
     1801 tgtcttatcg tctcaccccg tgggggtcaa cgaccggatt ctcgacgcga ccaaggtgtg
     1861 ctgtgagcgt tacggcatcg ccaaggtgag catcgacgac atcgccgccg aggcgggggt
     1921 gacgcgggcg acgttgtacc ggttgttccc cggcggcaag gacgtggtgt tcgacgagtt
     1981 cttcaaccgc atccagaccg cgctcgtcgg ggccgacacg ctcgaagacc tgctcgtcgg
     2041 cgccgtggtg gcggccacca acgacctgcg caacgaccac cacctggcga tcatgctgat
     2101 gtccgagccg ggcgagacgt tggccaacct caccgtcacc ggcatgccgc ggatcatccg
     2161 catggccacg ctgttcttgt caccgctggt gggcgcccac ctcgatcggc cccacgcgac
     2221 ccgcgtggtc gacgtgctgg cccgcctggt gatctcgtac ttcctcgccc cgtccgacga
     2281 cgtcgacctc ggcgatnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnng ccgttgcggc cgattgacag gagccatccc atgaccatca tgcacaacga
     2461 caacgagacc atcctcggcc gcggcgagat cgacgacatc gaagcgatcc tcgccatccc
     2521 ggtggtcgac cccgccgagg tcgagcacat cgtcaagaac aacgccgatt cgatcttcac
     2581 ctgggattac tccctggccc gcccggcgct gcgcaagctg tacgaaaagg ccaagagcgg
     2641 acagtggaac ggcaccgtcg acctcgactg gaagaccgac gtcgacgtcg aaaagagcgt
     2701 cgccgaagat caggccgtcc tcggtaccgg cttcgaccgc aacacctaca tcggcacgcc
     2761 ggtcgagaag tggggcgaca aggagtggct ggagttcggc atcgagggcc gcaactggca
     2821 gctcagccag ttcctccaag gcgagaaggg tgagctgctg tgcaccgcca agatcgtcga
     2881 gaccgtgccg tggtacgacg ccaaattgta cgccagcacc caggtgatgg acgaggcccg
     2941 ccacgtcgag gtgttcgccc gctacctcga cgagaagctc ggcggcggct accagatcaa
     3001 cgcccacctg cgactgctgc tggacgacat catcaacgac agccggtggg acatgaccta
     3061 cctcggcatg caggtgatgg tcgagggctt ggcgctggcc gcgttcgggt tcctgcacag
     3121 catcacccag gagccgctgc tcaagaagct gctccgctac gtgatgagcg acgaggcccg
     3181 ccacgtggcc ttcggtgtgc tcagcctgaa ggaggtctac gacggcatga ccgacacgga
     3241 gatcaaggac cgtcaggagt tcgcgttcga agccgccgtg cgcatgcgcg atcggttcat
     3301 gagccaagag gtgtgggagc ggatgggcat cgacatgcac gacatcatgc cgctgctgct
     3361 gcaagacccg acccggggca cgttccagga gatgctgttc agcaagatcg tgccgaactg
     3421 caagaagctg ggtctgctcg accgcaacga caagtggctc cggcaccgct tcgaggagct
     3481 cggcgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnngaga gagcccgtac ttctgcccct tccggcgcat
     3661 cctgatgtgg ggccgtcgcc ccgcgtgaac atcgcggtca gggccatgca tgtcgaggtc
     3721 agcagggtga gccgccgtcg ctggcggcga ggtagcctgg tcgctgatgt ctcgtccgac
     3781 gacccggcag tacgccagtg gagtcggacc gctcgccgct gtggcggtcg agttgtgcca
     3841 gtaccaggac atcgctgaaa caacaatgcg catcggcgcc gcgaccggcc tcagccacgc
     3901 tggtcgccac cacattgcgc cagccgctcg tctgcgggcc ggctgccctt cggaaggacc
     3961 gatttgagat ggcccgcttc ggccgtgttt tgaccgcgat ggtgaccccg ttcgacgnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnttgg ccaccgcggc attggcctgc atcgcgccgg acagcagccg cacagccacc
     4141 agcatccaca gcgcggtggc cgaggcggtg accattcccg tcgtcgccgg taccggcacc
     4201 aacgacacac cgcactccat ccacatgacc cgtgaggcca ccgcgctcgg cgccgccggc
     4261 gtgctcgccg tgtgcccgta ctacaaccgt ccttcccaag ccggcatcga ggctcatctg
     4321 cgggcgatgg ccgccgcgac cgatctgccg ttggtcgtct acgacatccc ggtgcgcact
     4381 ggacgcaaga tcgccacctc gacgctgctg cgcctgttcc gcgaggtgcc caacgtcgtt
     4441 gccctcaagg atgcggctgg caatcctgcc gagactgcga gcctgatcgc cgccgcgccc
     4501 aacggcgtcg aggtctatag cggcgacgac gcgatgaccc tgccgttgtt gtccgtcggc
     4561 gccgtcggtg tgatcggcgt cgccacacac tggtcgggtg acgaccacca gcagatgctc
     4621 gatctgtggg agaagggcga cgtcgtcggc gcgcgcatcg tcaacagccg cctgctggag
     4681 agcttcgcct acgagaccgg tgacgaggct cccaacccga tcccgaccaa ggcgatgatg
     4741 cgccacctcg gcattccggt cggccaggcc cgactgccga tgggcgacgc tcccgacttc
     4801 gtggtcgagc gcgcgccgca tgtgtggaac aacctggcac gatggcggga tgccttcccc
     4861 ctctgccctg ccgatgcctg atcacatctc gatcgtcttc cttggcggtc tcggtgagat
     4921 cggtcgcaac tgcatggcca tcgaccagca gggccaggtg ctgctgatcg attgcgggtt
     4981 gatgttcccc gacgccgaca tgcacggcat cgatctggtg ctgcccgatt tcacctggc
//
LOCUS       DAORNF010000156         5037 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_12361_length_5037_cov_1.723003, whole genome shotgun sequence.
ACCESSION   DAORNF010000156 DAORNF010000000
VERSION     DAORNF010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 5037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5037
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12361_length_5037_cov_1.723003"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="PLV68_07550"
     CDS             complement(<1..207)
                     /locus_tag="PLV68_07550"
                     /inference="COORDINATES: protein motif:HMM:NF021073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HRE01140.1"
                     /translation="MAFRLIPRTTQRPASNDAVGRGMEFALLVAMFLGLGYLLDRALG
                     TKPLFMIVLVVLGVIGQFASLWYGY"
     gene            complement(330..>888)
                     /locus_tag="PLV68_07555"
     CDS             complement(330..>888)
                     /locus_tag="PLV68_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HRE01141.1"
                     /translation="CLGFLPHNFNPAKIFMGDGGALLLGLLMACATSVVGGRTGTDPD
                     QAFFGQSYFFFAPLLIPLIILGVPIFDMAFAIVRRTTRQGFDTADKMHIHHRLMNLGH
                     GQRRAVLILWLWTALLSSMVLYPVYTTGGYTWAVVGALAMGLALYTVLHPEVRRNRNK
                     PTVEMSMGEASQPDDRDPGHDTVSV"
     assembly_gap    889..992
                     /estimated_length=104
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<993..1513)
                     /locus_tag="PLV68_07560"
     CDS             complement(<993..1513)
                     /locus_tag="PLV68_07560"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HRE01142.1"
                     /translation="MTSTAQYLIVGLCAMLVTFAVTPLVIRFAKHVGWVVEPDARRVH
                     TVTTPDVGGIAMFIGFVAALGLTRLMGGFDPLYARLSEIRGVLIAAAIIFMVGLIDDV
                     RELSAPAKVAGTVLAGSVLVWEGVTMYYFRLPLIGVYQWSNDWLPLVTVLWLLGISQA
                     INLIDGLDGLAAG"
     assembly_gap    1729..1754
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1755..>5037
                     /locus_tag="PLV68_07565"
     CDS             <1755..>5037
                     /locus_tag="PLV68_07565"
                     /inference="COORDINATES: protein motif:HMM:NF014869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HRE01143.1"
                     /translation="DPIAALAAEAGASLSVATDPETGQVSLVAPTGDRPLVAAAEGVD
                     PIEAAGAFAATFGAVFLTRPDPPASAPTRTERVASADDGVTPGTDQLVPGTVEIVSGT
                     VEIVSGTVEATVAAATQWVESRVTAGAEGSVAVRYQQHVGGIPVFAGEIAVQVAPDGS
                     VRSATGEASAVVSPPAEPQVTSAAAQGVALAAVAKALDGGVSADGLQATPAELWWYDP
                     QLIGAGGEPSLTLTWRTEVTGAGAFGTAATGSVRQTVLIDARTGGVALRIDNEPEALD
                     RLVCNNAGPSSYQSIACNTTQPGRVYRAEGGPATGEANTDTAYAVAGATYDYFAQFGR
                     DSLDDQGMPLQISVNFCPSSCGSDGYKNAYWSGTQAVFGKGYALADDIVAHELTHGVV
                     EHTAGLLYYSESGAINESFADIFGELIDRTTTASGADPAIDDWYIGEQIPTRYPGIRS
                     MKNPTASPYLDPDRMTSVLFHGAVSDNRGVHTNSGVGNKAAYLIAAGTGGEPGGSFNG
                     QTITGIGLAKTGLLYYTVLTQYLGAGSDYLDLYHGLQQACAALAQIPATTFTATDCAQ
                     VTKAVTVTEMHRAPIVPGARLTAPMCASDEARDTLFSDDFESGADKWRTSAIDLTTGL
                     SAPDGLWRLFGGSSQSGTRSLYGPNVDFSADTSARMADPITIPPGTTYLRFDHSFNLE
                     ASGSEYYDGGVVEYSTTGSNGPWLDTGNGATALPTVNGYTGRITTTSPSSTNPLRAKM
                     TFSGNSPGYQTTRIDVSALAGRSVLFRFRIGTDSYVAADGWFIDDVAVYRCGEQFIPT
                     GPVRVFDTRPGQSPSALVTVPKAGVGGDAGVLEVAMTGLAGVTPPTGVTAVSLNVTAT
                     EAAVAGFVTVYPCGQLPGSSSVNFQANSTVPNAVITPVSATGTVCFFANTPVHVVVDI
                     NGWIVGGTGFEPVGPARVFDTRANESPTAIRNVPKVRVGPDSVLTVQMADLDGFTPAT
                     GVAAVSLNVTVTGSTLPGFVTVYPCGSVPFVSSLNYTAGATVANAAIAPVSDAGTVCF
                     RASSPVDVVVDVNGWFAATPGHTPVGPVRVIDTRGESPEALVPGARTMIGPATPLVVT
                     VAGLGD"
BASE COUNT          852 a         1717 c         1628 g          710 t
ORIGIN      
        1 gtagccgtac cacaagctgg caaactggcc gatcacgccg agtaccacca agacgatcat
       61 gaacaacggc ttggtgccga gcgcgcgatc gagcaggtac cccagaccca agaacatcgc
      121 aaccagcaag gcgaactcca taccccgccc gaccgcgtcg ttggacgccg ggcgttgcgt
      181 ggtgcgcggg atgagtcgga aggccatgcg ggtgcaagtg cggccacggt gggccgtgac
      241 gccgatgtca cggagcttgt gaacgcggga acaatatagg tccgatcggc gaggtatccc
      301 aaatcgatga cgcgggtcag cgaagaaatc tagacgctca ccgtgtcgtg tccggggtca
      361 cgatcgtccg gttgcgacgc ctcgcccatc gacatctcaa cggtcggctt gttgcgattg
      421 cggcgcacct cgggatgcag cacggtgtac aaggccagcc ccatcgccaa tgccccgacc
      481 accgcccacg tatagccacc cgtggtgtac accgggtaca gcaccatgct cgacaacagc
      541 gcggtccaca gccacaagat cagcaccgcg cggcgctggc cgtgacccag gttcatcagc
      601 cgatgatgga tgtgcatctt gtcggcggtg tcgaagccct ggcgcgtggt gcggcgcacg
      661 atcgcgaacg ccatgtcgaa aatcggcaca ccaaggatga tcagcgggat cagcaacggc
      721 gcgaagaaga agtacgactg gccgaagaac gcctggtccg ggtcggtacc agtgcgacca
      781 ccgaccaccg acgtggcgca ggccatcagc agcccgagca gcagcgcgcc gccatcgccc
      841 atgaagatct tggccgggtt gaagttgtgc ggcagaaatc ccaggcagnn nnnnnnnnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnatgcccgc cgcgagcccg tcgagtccgt
     1021 cgatcaggtt gatcgcctgg ctgatgccca acaaccacaa cacggtcacc agcggcagcc
     1081 agtcgttgct ccattggtag acaccgatca gcggcagacg gaagtagtac atcgtcacgc
     1141 cctcccacac cagcaccgat ccggcgagga cggtgccggc gaccttggcg ggagcggaca
     1201 actcacgcac gtcgtcgatc aggccgacca tgaagatgat cgctgccgcg atcaatacgc
     1261 cgcggatctc gctgagccgg gcgtataacg gatcgaaacc gcccatcagc cgggtcaggc
     1321 cgagtgccgc gacgaacccg atgaacatcg caatgccgcc gacatcgggc gtggtgacgg
     1381 tgtgcacccg ccgggcgtcg ggctcgacca cccagccgac gtgcttggcg aaccggatca
     1441 ccagtggcgt gaccgcgaac gtgaccagca tcgcgcacag tccgacgatg aggtattgag
     1501 cggtgctcgt cacggccatg agcgtattcc aggccgcatc cggccggcag cacgtggcgg
     1561 ccagcgcgga ggcgccgcga acgcggttcg ccggctgcgg cggcatgaca gactgagcgc
     1621 catcactgta cgcatcgaag ttcgctcggc gcgcggaagg gcgagacggc cggcatgggc
     1681 agcgttgtgc gtcgccgcac tcgccacgac gctgtcgatc accggccgnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnngacccc atcgccgcac tcgccgccga ggcgggagca tcgctgtcgg
     1801 tcgcgaccga tccggagacg gggcaggtct cgttggtcgc acccaccggc gatcgacccc
     1861 tcgtcgccgc ggcggaaggc gtcgatccga tcgaagccgc cggagcgttc gccgcaacgt
     1921 tcggggccgt gttcttgacc cgaccggacc cgccagcgtc ggcgccgacc cgaaccgagc
     1981 gggtcgcgag cgcagacgat ggggtaacgc cggggaccga ccagttggtg cccggcaccg
     2041 tcgagatcgt gtccggcacc gtcgagatcg tgtccggcac cgtcgaagcg accgttgccg
     2101 cggccacaca gtgggtcgag agccgggtca ccgctggcgc cgaaggcagc gtggctgtgc
     2161 gctaccaaca gcacgtcggt ggcatcccgg tgttcgcggg cgagatcgcg gtgcaggtcg
     2221 cgcccgacgg atcggtgcgc tcggccacgg gcgaggcatc ggcggtggtg tcgcccccgg
     2281 ccgagccgca ggtcaccagc gcggcggccc aaggcgtggc actcgctgcg gtggccaagg
     2341 cactggatgg cggggtgtca gcagacgggc tgcaggccac cccggccgaa ctgtggtggt
     2401 acgacccgca gctgatcgga gccggcggtg agccgtcgct gacgctgacg tggcgcaccg
     2461 aagtcaccgg cgctggcgca ttcggcactg ccgcgaccgg ttccgtccgt cagaccgtgc
     2521 tgatcgacgc ccgtaccgga ggggtcgcgc tgcggatcga caacgagccc gaggccctcg
     2581 accgtctcgt ttgcaacaat gccggtccgt cgtcgtacca aagcatcgcc tgtaacacga
     2641 cccaacccgg tcgggtgtac cgcgccgagg gtggcccggc cactggcgag gccaacaccg
     2701 acactgcgta tgcggtcgcg ggagccacct acgactactt cgcccagttc ggtcgcgaca
     2761 gcctcgacga ccagggcatg ccgctgcaga tcagcgtcaa cttctgcccc tcatcctgcg
     2821 gctccgacgg ctacaagaac gcgtactggt cggggacaca ggcggtgttc ggcaagggat
     2881 atgccctggc cgacgacatc gtcgcccacg aactgaccca cggcgtggtc gagcacaccg
     2941 cggggttgct gtactacagc gagtcgggcg cgatcaacga gtcgttcgcc gacatcttcg
     3001 gtgagctgat cgaccgcacc accacggcca gcggagccga cccggccatc gacgactggt
     3061 acatcggcga gcagattcct acccgctatc cgggcatccg ctcgatgaag aacccgaccg
     3121 cctccccata tctcgacccc gaccggatga ccagcgtgct gttccacggt gccgtcagcg
     3181 acaaccgcgg ggtgcacacc aacagcgggg tcggcaacaa ggccgcgtac ctcatcgcgg
     3241 ccggcaccgg gggtgagccg gggggctcgt tcaacgggca gacgatcacc ggcatcgggc
     3301 tggccaagac cgggttgctc tactacaccg tgttgaccca gtacctcggg gcggggtccg
     3361 actatctgga cctgtaccac ggcctgcaac aagcgtgcgc cgcgctggcc cagattccgg
     3421 ccacgacgtt caccgcgacc gattgcgcgc aggtcaccaa agccgtaacg gttaccgaga
     3481 tgcaccgtgc cccgatcgtg cccggggccc gcctgacggc tccgatgtgt gccagcgacg
     3541 aggcgcgcga cacgctgttc agcgacgact tcgaatcggg cgctgacaag tggcggacgt
     3601 cggcgatcga cctgaccacc gggctgagcg cgccggacgg cttgtggcgg ctgttcggcg
     3661 gttcgtcgca gagcggcacc cgctcgttgt acgggccgaa cgtcgacttc agcgccgaca
     3721 ccagcgcacg catggccgac ccgatcacga tcccgccagg cacgacgtac ctccgcttcg
     3781 atcacagctt caacctggag gcgagcgggt cggagtacta cgacggcggc gtggtcgagt
     3841 acagcaccac cggctcgaac gggccgtggc tcgacacggg caacggcgcc acggcgctgc
     3901 cgacggtcaa cggctacacc gggcggatca cgacgacctc gcccagttcg accaacccgt
     3961 tgcgggccaa gatgacgttc tccggcaaca gccctgggta ccagaccacg cgcatcgacg
     4021 tcagcgccct cgcgggccgg agcgtgctgt tccgcttccg tatcggcacc gactcgtacg
     4081 tggccgccga cgggtggttc atcgacgacg tggccgtgta ccggtgcggc gagcagttca
     4141 tccccaccgg cccggtgcgg gtgttcgata cccgaccggg gcagtcgcca tcggcgttgg
     4201 tcaccgtgcc caaggctggc gtcggcggcg atgcgggcgt gctggaagtg gcgatgaccg
     4261 gcctggccgg ggtcacgccg cccaccgggg tcaccgccgt gtcgctcaac gtcaccgcca
     4321 cagaagcggc cgtggcggga ttcgtcaccg tctatccgtg cggccaactc cccggctcgt
     4381 ccagcgtcaa cttccaggcc aactcgaccg tgccgaacgc ggtgatcacc ccggtgtcgg
     4441 ccacgggtac cgtctgcttc ttcgccaaca cccccgtcca cgtcgtggtc gacatcaacg
     4501 gctggatcgt gggcgggacc ggcttcgaac ccgttggccc ggcccgcgtg ttcgacaccc
     4561 gcgccaacga gtcgccgacc gcgatccgca acgttcccaa ggtccgcgtc ggccccgact
     4621 cggtgctgac ggtccagatg gccgacctgg acgggttcac accggccacc ggggtcgcgg
     4681 ccgtgtcgct caacgtcacg gtcaccggct cgacgctgcc cggcttcgtc accgtctacc
     4741 cgtgcggctc cgtcccgttc gtgtcctcgc tgaactacac ggccggtgcc acggtcgcca
     4801 acgccgccat cgcgccagta tcggacgcgg gcacggtgtg cttcagggcc tcgtcgcccg
     4861 tcgatgtcgt agtcgacgtc aacggatggt tcgcggcaac gcccgggcac acgcccgtcg
     4921 ggccggtgcg cgtcatcgac acccgcggtg agtcacccga ggcgctcgtg ccgggcgcac
     4981 gcaccatgat cgggcccgcc accccgctgg tcgtcaccgt cgccggtctc ggcgatg
//
LOCUS       DAORNF010000157         5033 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_12372_length_5033_cov_1.416231, whole genome shotgun sequence.
ACCESSION   DAORNF010000157 DAORNF010000000
VERSION     DAORNF010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 5033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5033
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12372_length_5033_cov_1.416231"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    334..378
                     /estimated_length=45
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<379..>644)
                     /locus_tag="PLV68_07570"
     CDS             complement(<379..>644)
                     /locus_tag="PLV68_07570"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRE01144.1"
                     /translation="ELEITESAVLNDLPRARETLHNLQNMGVTLAVDDFGTGYASLNH
                     MAALPLDVLKVDRLYVQKMFTDPTAAAVVRFAVDLARHLNATVVA"
     assembly_gap    645..779
                     /estimated_length=135
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<780..>1413)
                     /locus_tag="PLV68_07575"
     CDS             complement(<780..>1413)
                     /locus_tag="PLV68_07575"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRE01145.1"
                     /translation="ARFKEINDTLGHDVGNDVLSQVADRLRSEFGDALVARTSTDGFA
                     MVKPGGDSAVATQLCDRIERAMLAPIAVRGIEMSVRADIGVALALPGVDRPDDALHHA
                     DIAMTAAGREHVGRRLYSPDLDEHSPERLRLAAEMREAIDSGQLSLVFQPKLDIVSCR
                     VDGVEAFVRWHHPQRGILMPGDFLATIENTELIGPLTWCVLDLALHHASAW"
     assembly_gap    1414..1436
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1437..>1914)
                     /locus_tag="PLV68_07580"
     CDS             complement(<1437..>1914)
                     /locus_tag="PLV68_07580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01146.1"
                     /translation="ALDQAVGGSTVAVVQSWDQSALAASSTAADSDTGEKVFTVFMPL
                     RANPDGTFTLDVPGADTDRTTPVVGAIEVNQPYAPIQSSVAHDSRYLALALLSGLLVV
                     YLVVLRLAIGASRRWRRQSMENEHRINHDDLTGLLNRRGLITAMTQALQRDTHVAQT"
     assembly_gap    1915..2004
                     /estimated_length=90
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2005..2303)
                     /locus_tag="PLV68_07585"
     CDS             complement(<2005..2303)
                     /locus_tag="PLV68_07585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01147.1"
                     /translation="MTWRGKSVGRKLPSLSMTVRFGLISLALFALVGLWLGGQLQGTL
                     RDRAHGEATANAQVLADVGLRPLLRQGAAPTELQVDPTASEQLDRALGSMMWSNGL"
     gene            complement(2391..>3430)
                     /locus_tag="PLV68_07590"
     CDS             complement(2391..>3430)
                     /locus_tag="PLV68_07590"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HRE01148.1"
                     /translation="IPAGVPADAVALALNITVTESSAAGFVTQFPAGRARPNSSVLNL
                     DRANQTRAAAGIFPVTGAGTSLFLSGGGHVIVDITGYFTGPSAAASTDGLFTAVDPTR
                     LLDTRGASPLGSGVPLYPAGGLDLAVGRGGAIAFNITAVDSGTGFLTAYPAGTARPAT
                     SSVNAAGDTVANFGVVQVSNRGLGVWSNMRTHALVDVQGWFSGPSAAATEPPPANTPP
                     PTPQPSYSSCNTEGLAFVNVKRSAPLVTNAAAEAWACAHALQLAASGSLTHSNSAARN
                     AAVGCGTGENIAYATGTSVSKLYSLWYNSAPHFANITNRDYRSMASAFVVRTSPDGTQ
                     MIWGVNVFALC"
     assembly_gap    3431..3532
                     /estimated_length=102
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3533..3961)
                     /locus_tag="PLV68_07595"
     CDS             complement(<3533..3961)
                     /locus_tag="PLV68_07595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01149.1"
                     /translation="MTAVGLTVTAARPAAAVSAAAAAPGSYTAVQPCRLTDTRTGAGV
                     IRIDPTTIEIAARQACGVPADATSVVVSIAIDAPAAAGFLSAWPADQARPTISNLNFS
                     AGQIRANSAIVPLSTTGRFRVYSNVITNVVVDVVGAFVPAQ"
     gene            complement(4184..>4692)
                     /locus_tag="PLV68_07600"
     CDS             complement(4184..>4692)
                     /locus_tag="PLV68_07600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01150.1"
                     /translation="QRRSGDDTTPSGILPLGVVHSPQGPISFFGNAADPGTRGPYRRV
                     QPGDCYGANPNTPGYGHWRVDPTGCTGDDELLARYVQSYAHAVLIGANTEPDVSGDAP
                     GETPYASAIFLHRSAYTPSGVAKATSGCVSVALPALVTAMRAIDPALSPNFAIGTRAA
                     LLAIRPAA"
     assembly_gap    4693..4814
                     /estimated_length=122
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4815..>5033)
                     /locus_tag="PLV68_07605"
     CDS             complement(<4815..>5033)
                     /locus_tag="PLV68_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01151.1"
                     /translation="VRQASDFLMALQLTGAANAADPAVRGARSALVLPPAPSVAGPAL
                     AGCDAPVVQALMESNPSVSQWIMVIAPAA"
BASE COUNT          700 a         1467 c         1653 g          696 t
ORIGIN      
        1 cccgtggctg agcccgatca cccgagccgg gctgacgggt ttgccatctg gtgaaccggc
       61 gtggtgtgcc cggcggatga gcaatcgggc ttccgcggag cgtggacgtg ccggaaaccg
      121 ggcaatcgac gatccgcgga cgctgaaatc accggatggc ggcattctcg atcgtcgcct
      181 caagcaggtg gctgacttcc accccgttac gacgcggcgg ctgtggcggg accgcgggtg
      241 gcggcgtacc agcggtggat ccagccgagg agttcttcgg cgggcatcgg ctgggcgatg
      301 agatggcctt gggcgtgggc gcagccgagg cggnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnngc gaccacggtg gcgttgaggt ggcgggccag gtcgacggcg
      421 aagcgcacca ccgctgccgc ggtggggtca gtgaacatct tctgtacgta gaggcgatcg
      481 accttcaaca cgtcgagtgg caaggcagcc atgtggttga gcgacgcgta gccggtgccg
      541 aagtcgtcga ccgccagcgt gacacccatg ttctgcaggt tgtgcagtgt ctcgcgggcg
      601 cggggcaggt cgttcaacac cgcactctcg gtgatctcca gttcnnnnnn nnnnnnnnnn
      661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc
      781 cacgccgacg cgtggtgcaa cgccaggtcg agcacgcacc aggtcagtgg cccgatcagt
      841 tcggtgttct cgatcgtggc caggaagtcg ccgggcatca ggatgccgcg ctgggggtgg
      901 tgccagcgca caaacgcctc gacgccatcg acccggcacg acacgatgtc gagcttcggc
      961 tggaacacca gcgacagttg gccgctgtcg atcgcttcgc gcatctcggc cgccaggcgc
     1021 agccgctcgg ggctgtgctc gtcgagatcg ggtgaataca gccgccgccc gacatgctcg
     1081 cgtccggcgg cggtcatggc gatgtcggca tggtgcagcg cgtcgtcggg ccggtcgaca
     1141 cccggcagag cgagggccac accgatgtcg gcgcgcacgc tcatctcgat gccgcgcacc
     1201 gcgatcggcg ccagcatcgc ccgctcgatg cgatcgcaga gctgggtcgc cacggcggag
     1261 tcgccaccgg gcttgaccat cgcgaacccg tcggtgctgg tgcgcgcgac cagcgcgtcg
     1321 ccgaactcgc tgcgcaggcg gtcggccacc tggctcagca cgtcgttgcc gacgtcgtgg
     1381 ccgagcgtgt cgttgatctc cttgaagcgg gcgnnnnnnn nnnnnnnnnn nnnnnncggt
     1441 ctgcgccacg tgcgtgtcgc gttggagcgc ctgcgtcatc gccgtgatca ggccgcgccg
     1501 gttcaacaga cccgtcagat cgtcgtggtt gatgcggtgc tcgttctcca tgctctggcg
     1561 ccgccagcgg cgtgacgcac cgatcgccag gcgcagcacc accaggtaca cgaccaacaa
     1621 cccgctcagc agggcgaggg cgagatagcg agagtcgtga gcgaccgacg actggatcgg
     1681 ggcgtacggc tggttgacct cgatcgcgcc gacgaccggt gtagtgcggt ccgtgtcggc
     1741 gccgggcacg tcgagggtga aggtgccgtc ggggttggcc cgcaggggca tgaacacggt
     1801 gaacaccttc tcacccgtgt ccgaatcggc ggcggtgctc gacgcggcga gcgcggactg
     1861 gtcccacgac tgcacgaccg cgacggtgct gccaccgacc gcctggtcga gcgcnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnaggccg ttcgaccaca tcatcgagcc gagggcgcgg
     2041 tccaactgct cgcttgcggt gggatcgacc tgcagttcgg tcggcgccgc gccctggcgc
     2101 agcaggggcc gcagccctac gtcggccagc acctgggcgt tggcggttgc ttcgccgtga
     2161 gcccggtcac gcagcgtgcc ctgcagttgg ccgcccaacc acaatccgac caacgcgaac
     2221 agggccaggc tgatcagccc gaaccgcacc gtcatgctca gcgagggcag cttgcgtccc
     2281 acgctcttcc cgcgccatgt catggccgag atatcggcac tcttggcgcg atcctgagga
     2341 tttcttgccg acacgcgacc ccggcgcgcg gaccccggca cgtcgcccgg tcagcagagg
     2401 gcgaacacgt tgacgcccca gatcatctgg gtgccgtcgg ggctggtgcg caccacgaac
     2461 gcactcgcca tgctgcggta gtcgcggttg gtgatgttgg cgaagtgcgg tgccgagttg
     2521 taccacagcg agtagagctt cgatacagac gtacctgtgg cgtaggcgat gttctcgccg
     2581 gtaccgcacc cgaccgccgc gttgcgagca gcagagttcg agtgcgtcag cgacccgctc
     2641 gccgcgagtt gcagggcatg ggcgcaggcc cacgcctcgg ctgctgcgtt ggtgaccaac
     2701 ggggccgagc gcttgacgtt gacgaaggcc agaccttcgg tgttgcacga cgagtacgac
     2761 ggctggggcg tgggtggtgg cgtgttggcc ggaggcggct cggtcgcggc ggcggacggg
     2821 ccagagaacc agccctgcac gtcgaccagc gcgtgcgtgc gcatgttcga ccacacaccc
     2881 agaccgcggt tcgacacctg gacgacaccg aagttggcga ccgtgtcgcc ggccgcgttg
     2941 accgacgacg tcgctgggcg agcagtgccg gccgggtagg cggtcaggaa gccggtgccg
     3001 ctgtcgacgg cggtgatgtt gaacgcgatc gctccgccgc gtcccaccgc cagatcgagc
     3061 ccaccagccg gatagagggg gacgccggag ccgagcgggc tggcaccgcg ggtgtcgagc
     3121 agccgggttg ggtcgacggc ggtgaacagg ccgtcggtgc tcgctgccgc agaggggccg
     3181 gtgaagtagc cggtgatgtc gacgatgacg tggccgccac cgctgaggaa cagcgaggtg
     3241 ccagcaccgg tgacggggaa gatgcccgcc gcggctcggg tctggttggc ccggtcgagg
     3301 ttgagcaccg acgagttggg gcgggctcgt ccggcgggga actgggtgac gaaaccggcc
     3361 gcgctcgact cggtgaccgt gatgttcagc gccagggcca ccgcatcggc cggtacgcca
     3421 gcggggatcg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnctgcgcgg
     3541 gtacgaacgc gccgaccacg tcgacgacga cgttggtgat cacgttgctg tagacccgga
     3601 atcggccggt cgtactgagt ggaacgatcg cgctgttggc cctgatctgg ccggcgctga
     3661 agttgaggtt cgagatggtt ggacgggctt ggtccgccgg ccacgccgag aggaaccccg
     3721 cggctgcggg ggcgtcgatg gcgatgctga cgacgacaga cgtagcgtcg gcgggtacgc
     3781 cacacgcttg ccgcgcggcg atttcgatcg tggtcggatc gatgcggatc acccctgcgc
     3841 cggtgcgggt gtcggtcagg cgacacggct gcacggcggt gtacgaaccg ggtgctgccg
     3901 cggcggcgct gacggcggcc gctggtcggg cggcggtcac cgtgaggcca acggcagtca
     3961 cggcaccgca ggccaagacc gccgccacca tcgtcgtcac cagccgcgcc acaatacacg
     4021 atggggaccg gggccgtctc acctgcagaa aagtagtcgc caggttgtct cgaaaatgtc
     4081 atgcaactgc aagaagtcgg acaagaagcg tcgacttcaa cacggtgcgt gagatcgacg
     4141 ggctaaacct cactgaccgt gagccgtcgg cccaacgact ccgtcaggct gccggtcgga
     4201 tcgccagcag cgccgcgcgg gtgccgatcg cgaagttcgg tgacagtgcc ggatcgatcg
     4261 cccgcatcgc ggtgaccagc gccgggagtg cgaccgacac gcaccccgaa gtcgccttgg
     4321 cgacaccgga cggtgtgtac gcgctgcggt ggaggaagat cgcgcttgcg tacggcgtct
     4381 cgcccggcgc gtcgccgctg acgtccggtt cggtgttggc gccgatcagc accgcgtggg
     4441 cgtagctctg cacgtatctt gcgagcagct cgtcgtcgcc ggtgcatccc gtcgggtcga
     4501 cccgccaatg gccgtaaccg ggggtgttcg ggttcgctcc gtagcagtca ccgggctgca
     4561 cgcgtcggta cgggccgcgc gtgccgggat cggcagcgtt gccgaagaac gagatcggcc
     4621 cctgcggcga gtgcaccacg cccagaggta aaatgcccga cggggtggtg tcgtcgccac
     4681 tgcgccgctg tgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4801 nnnnnnnnnn nnnngctgct ggcgcgatca ccatgatcca ttgtgagact gacgggttgg
     4861 attccatcag cgcttgtacg accggcgcgt cgcacccggc cagcgccggc ccggcgacgg
     4921 acggggctgg tggtagcacc aacgcgctgc gtgcgccccg caccgcgggg tcggccgcgt
     4981 tcgccgcgcc ggtgagctgc agcgccatca ggaagtccga cgcctggcgc acg
//
LOCUS       DAORNF010000158         5006 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_12472_length_5006_cov_1.212280, whole genome shotgun sequence.
ACCESSION   DAORNF010000158 DAORNF010000000
VERSION     DAORNF010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 5006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5006
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12472_length_5006_cov_1.212280"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="PLV68_07610"
     CDS             <1..384
                     /locus_tag="PLV68_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016445439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRE01152.1"
                     /translation="GHRQGSTGYFFEPTVVSGLRQNDEMIQNEIFGPVITVQQFSDED
                     EAVRWANGVEYGLASSVWTKDFGRAMRMSKRLDFGCVWINTHIPVVAEMPHGGFKKSG
                     YGKDLSAYSLEDYTRIKHVMANIDS"
     gene            416..>661
                     /locus_tag="PLV68_07615"
     CDS             416..>661
                     /locus_tag="PLV68_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01153.1"
                     /translation="MSGKETLAAGNTSGYNNQPIAGTRQRTSGEYVSEQGRTTGSDDV
                     IVLQHVRKQFANFTAVHDANFSIRRGEFFSMLGPSGCG"
     assembly_gap    662..671
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <672..>1126
                     /locus_tag="PLV68_07620"
     CDS             <672..>1126
                     /locus_tag="PLV68_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRE01154.1"
                     /translation="TTTLRMIAGFDLPTSGTVLLEGADVTNVAPYKRNVNTVFQQYAL
                     FPHMTIRDNVAFGLRSKGGIPEAEIKRRVGEMLEIVKLEQFAGRRPAQLSGGQQQRVA
                     LARALVNLPSALLLDEPLAALDLKLRQAMQIELKRIQREVGITFVFVTH"
     assembly_gap    1127..1248
                     /estimated_length=122
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1257..>1503
                     /locus_tag="PLV68_07625"
     CDS             1257..>1503
                     /locus_tag="PLV68_07625"
                     /inference="COORDINATES: protein motif:HMM:NF019998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HRE01155.1"
                     /translation="MPAEIVDRDGDDAVVQLDAGPRLRLPCNGAGVGDPATVMLRPER
                     LHISVDDPGTVNSVHGVINNSIFQGSELRLIIHLENRT"
     assembly_gap    1504..1549
                     /estimated_length=46
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1550..>2284
                     /locus_tag="PLV68_07630"
     CDS             <1550..>2284
                     /locus_tag="PLV68_07630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01156.1"
                     /translation="HNGDDVWVSWRPDAAFLLRGRSAIVGATTTDVDEVQATLDGKDA
                     VTTAAAKPDVSGSRVNRRALLIGGGVVAAGAVGALFLSGTGKSGSSSSGGGAGTIGGL
                     GGGADEVRILNWQAYIDPSEDGAVGTVDRFARETGIRTAYSEDFNDNNEVFNKQFQAN
                     IGQGGTMDFDIACPTYWMAARIKNLGWLDPIPYDLVPNYANLDPLYLNQAWDKGGKYN
                     LPWQAGMTGLAYNAKLCGRDLTSIND"
     assembly_gap    2285..2328
                     /estimated_length=44
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2329..>2728
                     /locus_tag="PLV68_07635"
     CDS             <2329..>2728
                     /locus_tag="PLV68_07635"
                     /inference="COORDINATES: protein motif:HMM:NF024739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRE01157.1"
                     /translation="SEMRDTVGLVMMAMGIDPSEIDEAGMNAALDRIQGAVDSGQIRQ
                     FTGNEYLDPLNNGNFVACIAWSGDIANSVTNPDVHFVIPDEGGMSWFDTMVIPKGAAN
                     AVAAAAWMNFVYDPVQAAQITKYNNFVSPVV"
     assembly_gap    2729..2803
                     /estimated_length=75
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2895..>3080
                     /locus_tag="PLV68_07640"
     CDS             2895..>3080
                     /locus_tag="PLV68_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01158.1"
                     /translation="MAKADTGGTNKALREGKIAPYVMTLPGMLWLYFFFIVPLVTLLK
                     IALSVKPDRNLPVYDLTW"
     assembly_gap    3081..3239
                     /estimated_length=159
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3240..>3594
                     /locus_tag="PLV68_07645"
     CDS             <3240..>3594
                     /locus_tag="PLV68_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRE01159.1"
                     /translation="MLGLVMLPFFTSFLLRTLAWQTLLADQGPVLDFLHGVRLVGLLD
                     WLGITTDGKLINTQTAVIGGLTYNFLPFALLPIYVSLEKVDVRLVDAASDLYSSFPRA
                     FRKVILPLSLPGVFAG"
     assembly_gap    3595..3744
                     /estimated_length=150
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3979..4098
                     /estimated_length=120
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4099..4656
                     /locus_tag="PLV68_07650"
     CDS             <4099..4656
                     /locus_tag="PLV68_07650"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRE01160.1"
                     /translation="CTVATALGSLIALALARYRMRGGAAINLLLVLPLTTPEIVMGAS
                     LFTLFFNQGVQRGFVTIVIAHTMFCLSFVKARLRGLDWTLEDAAMDLGATPWRTFNKI
                     TLPMITPGIVAAFMLSLALSIDDYIITSFIAGDVSTFPREVFDGAKNELPAQVHVLAT
                     MIMLVAITIIVGGTLVSNRRASRNA"
     assembly_gap    4672..4703
                     /estimated_length=32
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4785..>5006
                     /locus_tag="PLV68_07655"
     CDS             4785..>5006
                     /locus_tag="PLV68_07655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01161.1"
                     /translation="MRAKPSLSRAATVVAAVLSVPLFGLLADVGVVAAAPVEVPAPAI
                     RITEFSYQLRGFGEAEFVELTNVGTTSVNM"
BASE COUNT          755 a         1391 c         1345 g          757 t
ORIGIN      
        1 ggccatcggc agggcagcac gggctacttc ttcgagccga cggtcgtgtc cggcctgcgc
       61 cagaacgacg agatgatcca gaacgagatc ttcggtccgg tcatcacggt gcagcagttc
      121 agcgacgagg acgaggcggt gcggtgggcc aacggcgtcg agtacggcct ggcctcgtcg
      181 gtgtggacca aggacttcgg tcgggcgatg cggatgagca aacggctcga cttcggctgc
      241 gtgtggatca acacccacat cccggtcgtg gccgagatgc cccacggcgg cttcaagaag
      301 agcggttacg gcaaggatct gtcggcctat tcgctcgagg attacacgcg catcaagcac
      361 gtgatggcca atatcgacag ctagtcccgg gtgtgaacgg cgcgagaaat cacccgtgtc
      421 gggcaaagag acccttgccg caggcaacac gagcggctac aacaaccaac caatcgcagg
      481 cacacggcaa cgtacgtcag gggagtatgt gagcgagcaa ggacgaacga ccggttctga
      541 cgacgtcatc gtgctccagc acgtgcgcaa gcagttcgcc aatttcaccg cggtccacga
      601 tgccaacttc tcgatcaggc ggggcgagtt cttctcgatg ctcggcccgt ccggctgcgg
      661 cnnnnnnnnn ngaccaccac gttgcggatg atcgcgggct tcgatctgcc gaccagcggc
      721 acggtcttgc tcgaaggcgc cgacgtcacc aacgtggcgc cgtacaaacg caacgtcaac
      781 acggtgttcc agcagtacgc cctgtttcca cacatgacga tccgcgacaa cgtcgccttc
      841 ggtctgcgca gcaagggcgg catccccgag gccgagatca agcggcgtgt cggcgagatg
      901 ctcgagatcg tcaagctgga acagttcgca ggacgccgtc ctgcccaact ctccggcggt
      961 caacagcagc gagtggcact ggcccgagcg ttggtgaacc tgcccagcgc gttgctgctc
     1021 gacgaaccgt tggctgcgct cgacctcaag ctgcgccagg cgatgcaaat cgaactcaag
     1081 cggatccagc gcgaggtcgg catcacgttc gtgttcgtca cccacgnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncc aacctgatgc
     1261 ccgccgagat cgtcgatcgc gacggcgacg acgcggtggt ccaactcgac gctggacccc
     1321 gcctgcgcct gccttgcaac ggtgccggtg tcggtgatcc ggccacggtc atgttgcgac
     1381 ccgagcggct gcacatctcg gtcgacgatc caggaaccgt caactcggtc cacggtgtga
     1441 tcaacaactc gatcttccaa gggtcggaac tgcggttgat catccacctc gagaaccgca
     1501 ccgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt gcacaacggc
     1561 gacgacgtgt gggtcagctg gcggcccgac gcagcgttcc tgctgcgcgg ccgctcggcc
     1621 atcgtcggcg ccaccaccac cgatgtcgac gaggtgcagg ccaccctcga cggcaaggat
     1681 gccgtcacga ccgcggcggc caagccggac gtgagtggtt cccgtgtcaa ccggcgagcg
     1741 ttgctgatcg gcggcggtgt ggtcgcggct ggcgcggtcg gcgccttgtt cttatcgggt
     1801 accggcaagt cgggcagttc ctcgtcgggc ggcggtgcgg gcaccatcgg tggactcggc
     1861 ggtggcgccg acgaggtgcg catcctcaat tggcaggcct acatcgaccc gagcgaggac
     1921 ggtgcagtcg gcaccgtcga tcgtttcgcg cgggagaccg gcattcgcac ggcgtactcc
     1981 gaggacttca acgacaacaa cgaggtcttc aacaagcagt tccaggccaa catcggtcag
     2041 ggcggcacga tggacttcga catcgcctgc ccgacctact ggatggcagc gcgcatcaag
     2101 aacctgggct ggctcgaccc gatcccgtac gacctcgtgc cgaactacgc caacctcgat
     2161 ccgctgtatc tcaaccaggc gtgggacaag ggtggcaagt acaacctgcc gtggcaggca
     2221 ggcatgaccg gcctggccta caacgccaag ctgtgcggtc gcgacctgac cagcatcaac
     2281 gaccnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnca gcgagatgcg
     2341 cgacaccgtc ggactggtga tgatggcgat gggcatcgac ccgtccgaga tcgacgaggc
     2401 aggcatgaat gccgcgctcg acaggattca gggcgcggtc gacagcggcc agatccgcca
     2461 gttcacgggc aacgagtacc tcgatccgct caacaacggc aacttcgtcg cgtgcattgc
     2521 gtggtcgggc gacatcgcca actcggtcac caaccccgac gtccacttcg tcatccccga
     2581 cgagggcggg atgagctggt tcgacacgat ggtgatcccg aagggagcgg ccaacgctgt
     2641 cgccgccgcg gcgtggatga acttcgtcta cgaccccgtg caggccgcgc agatcacgaa
     2701 gtacaacaac ttcgtcagcc cggtggttnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngaaggcc cggctgcggg
     2821 tgttcgccga catccccgac gagctcgacg ccacgatcac cgaccgcttc ctgcgcatca
     2881 ccggggcgtg agtcatggcc aaggctgaca ctgggggcac gaacaaggcg ctgcgggaag
     2941 gcaagatcgc gccgtacgtc atgaccttgc cgggcatgct ctggttgtac ttcttcttca
     3001 tcgtcccact ggtgacgctg ttgaagatcg ccttgtcggt caagcccgac cgcaacctgc
     3061 cggtgtacga cctgacctgg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng
     3241 atgctcggct tggtgatgtt gccgttcttc accagcttcc tgttgcgtac gctggcgtgg
     3301 cagaccctgt tggccgacca gggtcccgtg ctcgacttct tgcacggcgt ccgtctggtc
     3361 gggttgctcg actggctcgg catcaccacc gacggtaagt tgatcaacac ccagaccgcg
     3421 gtgatcggcg ggttgaccta caactttctc cccttcgcgc tgttgccgat ctacgtcagc
     3481 ttggagaagg tcgacgtgcg tctggtcgac gccgcgagcg acctgtacag cagctttcca
     3541 cgggcattcc gcaaggtgat cctgcccctg tcactgccgg gggtgttcgc cggcnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnncgtgct gatggcgatc atcaccgccg cggtgctgat
     3781 atacgccaag ttcctcggaa cggaggacct ggtgtgagcg ccttcccgca gagcagcagc
     3841 ccgcagcggg gcaagcgggt caagcccggc acgatcgcgc tgtacgtcgt gactgccggt
     3901 ggcctgttgt acctgttcgt gccgatcatc acgattgcgg tgttcagctt caacgacctt
     3961 cggctcaaga accccgacnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnntg caccgtcgcg accgcgctgg gcagcctgat cgcgctcgcg
     4141 ctggcccgct accgcatgcg tgggggagcg gcgatcaacc tgctgctcgt gttgccgctg
     4201 accacacccg agatcgtgat gggcgcctcg ctgttcacgc tgttcttcaa ccagggcgtg
     4261 cagcgcggct tcgtcacgat cgtcatcgcc cacacgatgt tctgcctcag cttcgtcaag
     4321 gcccgattgc gcggcctgga ttggaccttg gaggacgcgg cgatggatct gggggccacg
     4381 ccgtggcgaa ccttcaacaa gatcacgctg ccgatgatca cccccgggat cgtggccgcg
     4441 ttcatgctct cgctggcgct gtcgatcgac gattacatca tcacttcgtt catcgcgggc
     4501 gacgtcagca ccttcccccg cgaggtgttc gacggagcca agaacgagct gccggctcag
     4561 gtacacgtgt tggcgacgat gatcatgctg gtcgcgatca cgatcatcgt cggcggaacg
     4621 ctcgtctcca atcgccgcgc cagccgcaac gcctgaccgc tggcgacggt cnnnnnnnnn
     4681 nnnnnnnnnn nnnnnnnnnn nnnggccaac ggttcatcga cggacgccga gggttcacct
     4741 caactccacg ctggcgtcga tcggtaccgg tttgatcgcg gatcatgcgt gccaaaccct
     4801 cgctctctcg cgccgccacc gtggtcgcgg ctgtcctttc cgttcccttg ttcggtctgc
     4861 tcgccgacgt cggcgtcgtt gccgcggcac cggtcgaggt gccggcgcca gcgatacgga
     4921 tcacggagtt ctcctaccag ctccggggct tcggcgaggc ggagttcgtc gagttgacca
     4981 acgtcggcac gacgtcggtg aacatg
//
LOCUS       DAORNF010000159         4968 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_12598_length_4968_cov_1.686546, whole genome shotgun sequence.
ACCESSION   DAORNF010000159 DAORNF010000000
VERSION     DAORNF010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4968
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12598_length_4968_cov_1.686546"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    340..456
                     /estimated_length=117
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<457..807)
                     /locus_tag="PLV68_07660"
     CDS             complement(<457..807)
                     /locus_tag="PLV68_07660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01162.1"
                     /translation="MLVRNLTSAHPVWSRLWAVAILVGLSLTGSVAEARATGPVGAPL
                     GSLGFVAVQPARLLDTRAGFQTIDELSAGVGAVGAGEQLSVRVAGRAGIPASHVSAVV
                     LNVTAVDATERSFLT"
     assembly_gap    1113..1131
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1132..>1910)
                     /locus_tag="PLV68_07665"
     CDS             complement(<1132..>1910)
                     /locus_tag="PLV68_07665"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRE01163.1"
                     /translation="SFEQLVVANPTLVYCAITGYGEDGPWAARPAHGLQPDLCAGAVP
                     IDWTDGVPKIPAGYKAHGATLAGLWAALGITAGLLRRPVLNAAQYVSVSLWEAALAWM
                     WREGVNDLNGLDQRPDFQELGSRYRMYVGSDSAAILVCPTEEKFWVRFVDELGLPESW
                     RSIGDWTTGADNGFGREQEAAAIAERIATAPAHEWDARLTAAGVPVAVVQSVGDAYRS
                     PQAVARGSTVRVDGQDGAVYIPLSPLSVSAAERLSDGSVVR"
     assembly_gap    1911..1990
                     /estimated_length=80
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1991..2204)
                     /locus_tag="PLV68_07670"
     CDS             complement(<1991..2204)
                     /locus_tag="PLV68_07670"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRE01164.1"
                     /translation="MHHPRVIDFSTHLSGPTASRELVHLGFDVIKVERPRHGDGNRSM
                     GAQVAGASAVHHALSSGTRSVVLDRTS"
     gene            complement(2406..>3967)
                     /gene="cysC"
                     /locus_tag="PLV68_07675"
     CDS             complement(2406..>3967)
                     /gene="cysC"
                     /locus_tag="PLV68_07675"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HRE01165.1"
                     /translation="EQYTRNMITGASTADAAVILIDARKGVITQTRRHSYLVSLLGIR
                     RIVLAVNKLDLVGFSEEIFRSIVAQYEEFAAEIGLHDVVAIPISALLGDNITVNSANT
                     PWYDGPSLISHLETVPIDSTARVGPFRFPVQWVNRPSSEFRGFSGTVVGGSIRVGDAV
                     RVLPSGTTSRIATISTFDRDLDEAVEGQAVTLCLTDEVDVSRGSVICAADDPAGIADQ
                     FEAHIVWMHEAPLQPGRQYLLKSGPTTAAVTISRPKDKIDVNTLERLAARTLALNEIG
                     VCTITTDRPIPFDTYHANRDTGGFVVIDRMTNATVGAGMINFALRRASNVQWQALDVT
                     PEARASMKGHQPGVVWLTGLSGAGKSTIANGLERSLHARGVHTFLLDGDNVRHGLNRD
                     LGFTEADRIENIRRVVEVARLMIDAGLVVIVSFISPFRAERERARQAIGDGRYVEIFV
                     DAPLAVAEQRDPKGLYAKARNGLLPNFTGIDSPYEPPPDPDIHIDTTTVKPADAAETI
                     VAHLVATGMIH"
     assembly_gap    3968..4036
                     /estimated_length=69
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4037..4316)
                     /locus_tag="PLV68_07680"
     CDS             complement(<4037..4316)
                     /locus_tag="PLV68_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRE01166.1"
                     /translation="MNATPNNFVDLDLIASDIEAYLEAHEHKSLMRFITCGSVDDGKS
                     TLIGRLLYDTHLVFEDHLAALEADSKRFGTQGAELDLALLVDGLTAERE"
     gene            complement(4316..>4968)
                     /gene="cysD"
                     /locus_tag="PLV68_07685"
     CDS             complement(4316..>4968)
                     /gene="cysD"
                     /locus_tag="PLV68_07685"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009336 - sulfate adenylyltransferase
                     complex (ATP) [Evidence IEA];
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA];
                     GO_process: GO:0070814 - hydrogen sulfide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HRE01167.1"
                     /translation="LLIHKNPECIAQDINPFDHGSAMHTDMWKTEGLKQALDHFGFDA
                     AFGGARRDEEKSRAKERIFSIRSAQHRWDPKQQRPELWRIYNVRRAPGETMRVFPLSN
                     WTELDVWQYIHRESIPIVPLYLAAPRPVVERGGALIMVDDDRMRLDPGEQPQIKSVRF
                     RTLGCYPLTGAVESNATTLNEIIQEMLLTTSSERQGRVIDFDSSGSMEKKKQEGYF"
BASE COUNT          802 a         1556 c         1519 g          806 t
ORIGIN      
        1 cccggcgtca ccgtgcccgg tgccgggttg accgacgatg cccacggacg agccgatccg
       61 gtcggccaca ccgtcacgaa cgacggctcg gtcgcgccca ccgcggtgac gttcaacacc
      121 accgccccag cgcccaccgc cggcaccccg gatcgcccgg cgacgcgaac cgacaccgac
      181 cgaccggcac cgatggcacc agcggactgg ccctcaccat cgccggtggt gccatcgatt
      241 cgggtgtcca gcacccgagc cggagacacc ggcgtgaagg cagggccgac cggaaaccaa
      301 ccttgtacgt cgaccacgac gtcgaccgaa cccgacgcgn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncgtc aggaacgacc gctcggtcgc
      481 gtcgaccgcg gtgacgttca gcaccaccgc gctgacatgc gacgccggga tgccagcccg
      541 gccggccacg cgcaccgaca actgttcccc cgccccgacc gcgccgacac ccgccgataa
      601 ctcatcgatg gtctgaaagc cggcacgagt gtcgagcaac cgcgctggct ggactgcgac
      661 gaagcccagg ctgccgaggg gtgcgccgac agggcctgtc gctctggctt ccgccacgga
      721 accagtgagg gacagcccga cgaggatggc cactgcccac agacgtgacc acaccgggtg
      781 tgcggaggtc aggtttcgga cgagcatcca tgtcaccgtt ctcgtgcgac ctcggtggct
      841 gcacgtcaaa cccgtccgtc ggtgaggatg tcacggcccc gcacggcccc gcaactattc
      901 tcgaactttt atttgtattt tcacttgcag tgcaagtagt tctgacccct gtcagggacc
      961 aaccgtgccg actgtgtcaa gcacggcccg tctcggggtg cgcaacggcg gccgctactg
     1021 ctctcgcggc tgcagcccga gctcgcgcag gatcgcctgg gtgtcatgac caaggcgcgg
     1081 ggcgggcgtc aggttggctc cacggtgtcg atnnnnnnnn nnnnnnnnnn nggcgcacga
     1141 ccgaaccatc gctcagtcgc tccgcggcgc tcaccgacag cggtgagagc ggtatgtaga
     1201 ccgctccgtc ctgcccatcc acgcgaacgg ttgatccgcg cgccaccgcc tgcggcgatc
     1261 gataggcgtc accgaccgat tggacgaccg cgacgggcac gcccgcggca gtgagccgcg
     1321 catcccactc gtgagccggc gccgtggcga tccgctccgc gatcgcggca gcctcttgct
     1381 cgcgaccgaa accgttgtcc gcaccggtgg tccagtcccc gatcgaccgc cacgattcgg
     1441 gaagacccaa ctcgtcaacg aaacgaaccc agaacttctc ctcggtggga cacacgagga
     1501 tcgcagccga gtcactgccg acgtacatgc ggtatcggga gccgagttct tggaaatctg
     1561 ggcgttgatc gagcccgttg agatcgttga cgccctcacg ccacatccat gccaacgctg
     1621 cttcccagag cgagacgctg acatattgcg ctgcgttcag taccgggcgt cggagcagac
     1681 ctgcggtaat gcccaacgcg gcccacagtc cggccagcgt ggcgccgtgc gccttgtaac
     1741 cagcaggaat cttcggcaca ccgtctgtcc agtcgatggg taccgcaccg gcgcagagat
     1801 cgggctggag tccatgcgca ggacgtgctg cccaaggtcc gtcttcgccg tatccggtga
     1861 tcgcgcagta gacgagggtg ggattggcga ccaccagttg ctcgaagctc nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn ggctcgtgcg gtcgagtacc acgctgcgcg tgccgctgct gagagcgtgg
     2041 tggacggcgc tggcgccagc cacctgggcg cccatcgacc ggtttccgtc accatgccgt
     2101 ggacgttcga ccttgatgac atcgaagccc aggtgaacca gctcacggct tgccgtcgga
     2161 ccggaaagat gggtcgaaaa gtcgatgacg cgcgggtgat gcaacctcga tgatgaatcc
     2221 atggcaatct ctcgtcgctc gcgacctcgg gccatgatgc gatcggtcgg ccgcgctggc
     2281 aaggaccagt tgccgaccag tgggacggtc cggacagcct cggcccgcga ccagccaccg
     2341 acgacccggt tgcctcgttc gtctcccagg aactcacgtg cacccgatcg ggtcgagtcc
     2401 ggcggctagt gaatcatgcc tgtcgcgacg aggtgagcaa cgatggtctc tgccgcgtcg
     2461 gcaggcttca cggtggtggt gtcgatatgg atgtcggggt ccggcggcgg ctcgtagggc
     2521 gagtcgatcc ccgtgaagtt ggggagcaat ccgtttcgcg ccttcgcgta caggcccttc
     2581 ggatctcgct gctcggccac cgcgagcgga gcatcgacga agatctcgac gtatcggccg
     2641 tcgccgatcg cctgccgagc acgttcgcgc tcggcccgaa acggtgagat gaacgacacg
     2701 atgacaacca gtccggcgtc gatcatcaac cgcgccacct ccaccacgcg gcggatgttc
     2761 tcgatccggt cggcttcggt gaagccgagg tcgcgattca ggccgtggcg cacgttgtcg
     2821 ccgtcgagca ggaaggtatg caccccgcga gcgtgcaggc tgcgctccaa cccgttggcg
     2881 atggtcgact tgcccgctcc cgacaggccg gtcagccaca ccacacccgg ctggtgaccc
     2941 ttcatcgacg cgcgcgcctc gggcgtcacg tcgagcgctt gccattgcac gttggacgca
     3001 cggcgcagcg cgaagttgat catgccggcg ccaaccgttg cgttggtcat ccggtcgatc
     3061 accacgaagc cgccggtgtc gcggttggcg tggtaggtgt cgaagggaat cggccggtct
     3121 gtggtgatgg tgcacacgcc gatctcgttg agggcgagcg tgcgcgccgc gagccgctcc
     3181 agcgtgttga cgtcgatctt gtccttcggt cggctgatcg tcacggcggc agtggtcggt
     3241 ccggacttca gcaggtattg acgaccgggc tgcagcggtg cttcgtgcat ccagacgatg
     3301 tgggcctcga actggtcagc gatgccggcg gggtcgtcgg cggcacagat cacgctgccg
     3361 cggctcacgt cgacttcatc ggtgagacac agcgtgaccg cttgcccctc gaccgcctcg
     3421 tcgaggtcgc gatcgaacgt cgagatcgtt gcgatccgac tcgtcgtccc cgacggcaac
     3481 acccggaccg cgtcacccac tcggatcgat cccccgacca ccgtgcccga aaagccgcgg
     3541 aactccgaac tgggacggtt gacccactgc accggaaacc ggaacggacc gacgcgtgcg
     3601 gtcgagtcga tcggcaccgt ctcgagatga gagatcagcg agggaccgtc gtaccacggc
     3661 gtgttcgccg aattgacggt gatgttgtcg ccaagcagcg ccgagatcgg gatcgcgacg
     3721 acgtcgtgca ggccgatctc ggcggcgaac tcctcgtact gcgcgacgat cgatcgaaag
     3781 atctcttcgg agaagccgac gaggtcgagc ttgttgacgg cgagcacgat gcggcggatg
     3841 ccgagcagcg acacgaggta gctgtgccgg cgggtctggg tgatgacacc cttgcgggca
     3901 tcgatgagga tgaccgcggc gtcggcggtg gacgctccgg tgatcatgtt gcgggtgtac
     3961 tgctcgtnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnngttc acgctcggcg gtcagaccgt cgaccagcag ggccagatcg
     4081 agttctgccc cctgggtgcc gaaccgcttc gagtccgcct ccaacgcggc gaggtggtcc
     4141 tcgaacacga gatgggtgtc atacagcagt cgaccaatga gcgtgctctt gccgtcgtcg
     4201 acactgccgc aggtgatgaa cctcatcaag ctcttgtgtt cgtgcgcttc gaggtaggcc
     4261 tcgatgtcgc tcgcgatcag atcgagatcg acgaagttgt tgggcgtggc gttcatcaga
     4321 agtagccctc ctgcttcttc ttctccatgg atccggacga gtcgaaatcg atcacccgtc
     4381 cttggcgttc tgacgatgtc gtcagcaaca tctcctggat gatttcgttc aacgtggtcg
     4441 cgttgctctc gaccgcaccc gtgagcggat agcagccgag cgtgcggaac cggacgctct
     4501 tgatctgcgg ctgctcaccg ggatcgagcc gcatccggtc gtcgtccacc atgatcagcg
     4561 cgccgccgcg ttcgacgacg ggacgaggtg cggcgaggta caacggcacg atcgggatgg
     4621 actcgcgatg gatgtactgc cagacgtcca actcggtcca gttcgacaag gggaacaccc
     4681 gcatcgtctc gcccggcgcc cggcggacgt tgtagatccg ccacagctca ggccgttgct
     4741 gcttggggtc ccaccgatgt tgtgccgagc ggatcgagaa gatccgctcc ttggcccgcg
     4801 acttctcctc gtcgcgccgg gcaccgccga acgcggcgtc gaagccgaag tggtcgagtg
     4861 cctgcttcag cccttcggtc ttccacatat cggtgtgcat cgccgaaccg tggtcgaacg
     4921 ggttgatgtc ctgggcgatg cactcggggt tcttgtggat cagcagtt
//
LOCUS       DAORNF010000160         4941 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_12694_length_4941_cov_1.480761, whole genome shotgun sequence.
ACCESSION   DAORNF010000160 DAORNF010000000
VERSION     DAORNF010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4941
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12694_length_4941_cov_1.480761"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="PLV68_07690"
     CDS             complement(<1..410)
                     /locus_tag="PLV68_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRE01168.1"
                     /translation="MNHAVLYVRDARRTERFYREVLGFSTVIEQPEGAYVFMRAPGSD
                     NHHDIAFFTIGAQAAPSEAGRRSVGLYHIAWEVESLDELEQMRTKLAGAGALVGASDH
                     GTNKSLYAQDPDGIEFEVMWLVPAEHWGASEHTAV"
     gene            complement(487..1626)
                     /locus_tag="PLV68_07695"
     CDS             complement(487..1626)
                     /locus_tag="PLV68_07695"
                     /inference="COORDINATES: protein motif:HMM:NF015056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HRE01169.1"
                     /translation="MALDITARLAAPGSGARHDLSASPSNCYWGYIDRDQPPALKVAS
                     GDVITVEAVTHHAGDAPDYLMDDGIRAIWDAIPAAERGPGVHLLTGPIHVDGAEPGDT
                     LVVRILDMAPRLAYGSNCAANWGLLYDIFQKERITIYGLGGHGPDGYGPDGYDPDGAA
                     GHDWGSAGGFPASARPMFGFDFTARPLYDLPGVISPPDSAARSPFSVPVTVPVRPHFG
                     VMGVAPAESGRHSSIPPGVWGGNVDNWRLGPGATMCYPVFVDGGLLYVGDPHFAQGDG
                     EICGTAIEASLNATIQVSVVKGLGVQSPLMETGTHWLTHGFGADLDAATRMAAEQMLW
                     LLQTHIGLTADDAYSLASVAMDLGVTQVVDRTVGCHASIARGIFA"
     gene            complement(1849..2019)
                     /locus_tag="PLV68_07700"
     CDS             complement(1849..2019)
                     /locus_tag="PLV68_07700"
                     /inference="COORDINATES: protein motif:HMM:NF025379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rv0909 family putative TA system antitoxin"
                     /protein_id="HRE01170.1"
                     /translation="MGLLDKVKGLFKGKKKQINQGVDKAADMAKAKAPDKFDDKIDTA
                     ADGIKKVTEKLD"
     gene            2183..>2683
                     /locus_tag="PLV68_07705"
     CDS             2183..>2683
                     /locus_tag="PLV68_07705"
                     /inference="COORDINATES: protein motif:HMM:NF019976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease-related domain-containing protein"
                     /protein_id="HRE01171.1"
                     /translation="MRPSDDIGGEPRKAERLQQNAHEWDKGKAGERMVAAALDALPHD
                     YRVYHHVGLPGTKEALDHVVVGPCGVVAINDTYVDGDLQHGSGTLWRGKVPIRRETEN
                     VRWEADVLSTALQQPVDAVLCCAEATLPEPVVTLDRTAVVSLDALTGHIITKPAKFDA
                     DAIAVVA"
     assembly_gap    2684..2696
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2697..>3292
                     /locus_tag="PLV68_07710"
     CDS             <2697..>3292
                     /locus_tag="PLV68_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01172.1"
                     /translation="VAPWVRTATARGVVSVLTTPDVTTGSERPGALPRREIRTPRRTR
                     SQIRQERAAQRFTGIEQPPREKGNYGVAVSALFAIAVLFGVWALWSRLGDGTQRAASP
                     TTTATPTVPPIITDAATTTTAAATTTTAPAASVPLPPNVEFDCPSPGKGWQATLIASE
                     FLQDPQGYHVWYSVRLSPLTYWGLFKSGLRTPEPIGPV"
     assembly_gap    3293..3302
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3522..3992)
                     /gene="rpsG"
                     /locus_tag="PLV68_07715"
     CDS             complement(3522..3992)
                     /gene="rpsG"
                     /locus_tag="PLV68_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HRE01173.1"
                     /translation="MPRKGPAERRKIMPDPIYRSLLVSQITNKVMLHGKRSIAEKIVY
                     DALKIVEDKTGSEPIATLKRAVDNIKPPLEVRSRRVGGATYQVPVEVRAPRANTLAIR
                     WLVDFSRKRREKTMAECLAGELLDAANGLGAAVKRRDDMQKMAESNRAFAHYRW"
     gene            complement(4019..4390)
                     /gene="rpsL"
                     /locus_tag="PLV68_07720"
     CDS             complement(4019..4390)
                     /gene="rpsL"
                     /locus_tag="PLV68_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020488528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HRE01174.1"
                     /translation="MPTIQQLVRQGRSSKFSKTKTPALKGSPQRRGVCTRVYTNTPKK
                     PNSALRKVARVRLSSGVEITAYIPGEGHNLQEHSIVLVRGGRVRDLPGVRYKIIRGAL
                     DAAGVKNRKQSRSRYGAKTPK"
     assembly_gap    4563..4576
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4577..>4941)
                     /locus_tag="PLV68_07725"
     CDS             complement(<4577..>4941)
                     /locus_tag="PLV68_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01175.1"
                     /translation="DAKIFRDTNKRMVEEGQRPAEGRPELMGITKASLATESWLSAAS
                     FQETTRVLTEAAIDGRSDGLLGLKENIIIGKLIPAGTGMERYRDFEVSAPDHRPMEFY
                     SSDIDDASAYLANLHGTQQ"
BASE COUNT          778 a         1677 c         1622 g          827 t
ORIGIN      
        1 acagcggtgt gctcggacgc accccaatgc tcggcgggca ccagccacat cacttcgaac
       61 tcgatgccgt cggggtcttg cgcatacagg ctcttgttgg tgccatggtc gctggcgccg
      121 accagggcgc ccgcgccagc gagcttggtg cgcatctgct cgagctcgtc gagcgactcg
      181 acctcccacg cgatgtgata cagcccgaca ctgcgccggc ctgcctcgct gggcgcggcc
      241 tgggcaccga tcgtgaagaa cgcgatgtcg tggtggttgt ccgaccccgg cgcgcgcatg
      301 aatacgtacg ctccttcggg ctgttcgatg acggtgctga agcccagcac ctcgcgatag
      361 aagcgctcgg ttcggcgagc gtcacgcacg tagagcacgg cgtgattcat ccttgtgatc
      421 ggcacgcgat ccctcctctt ggactgatgg tcagaccata gcaaaaggtg aggccatctc
      481 aacgagtcag gcgaagatgc cgcgggcgat cgaagcgtgg cagccgacgg tccgatcgac
      541 aacctgggtg accccaaggt ccatcgccac cgatgccagc gagtaggcat cgtcggcggt
      601 caggccgatg tgggtctgca acaaccagag catctgctcg gcggccatcc gcgtcgccgc
      661 gtcgagatcg gcaccgaaac cgtgggtcag ccagtgtgtg ccggtctcca tcagcggcga
      721 ctgcacgccg aggcccttga cgaccgagac ctggatcgtg gcgttgagcg atgcctcgat
      781 cgcggtgccg cagatctctc cgtcgccctg ggcgaagtgc gggtcgccga cgtagagcag
      841 cccgccatcg acgaacaccg ggtagcacat ggtcgcgccc ggcccgaggc gccagttgtc
      901 gacgttgccg ccccacacac cgggcgggat cgaggagtgc cggcctgact ccgcgggagc
      961 gacccccatc acgccgaagt gcggccgcac cggcacggtg accggcaccg agaacgggct
     1021 gcgggccgcg ctgtccggcg gcgagatcac accgggcagg tcgtacagcg ggcgggcggt
     1081 gaagtcgaag ccgaacattg ggcgcgcact ggccgggaac ccacccgccg agccccagtc
     1141 gtgcccggct gcaccgtccg ggtcatagcc gtcgggtccg tatccgtccg gcccgtgacc
     1201 gccgaggccg tagatggtga ttcgttcctt ctggaagatg tcgtagagca acccccagtt
     1261 ggccgcgcag ttgctgccgt acgccaggcg gggcgccatg tcgaggatgc gcaccaccaa
     1321 cgtgtcgccg ggctcagcgc cgtcgacgtg gatcggccct gtcaacaggt ggacgccggg
     1381 gccgcgctcg gccgcgggga tcgcgtccca gatcgcacgg atgccgtcgt ccatcagata
     1441 gtccggggcg tcgccagcgt gatgggtgac cgcttcgacg gtgatcacgt ctccgctggc
     1501 caccttcagt gccggcggct ggtcgcggtc gatgtacccc cagtagcagt tcgatgggct
     1561 cgccgaaagg tcgtgcctcg ctcccgagcc cggggccgcc agacgagcag tgatgtccaa
     1621 agccatgccg gcgacggtac gaaccgcatg tttccagcct gtttctcccc cacgtccatc
     1681 cgttgattct cggcccgacg gtcagaccat cagcccaccc acgatcggtg accctcacag
     1741 cagcgtgggg accgcgtgtc tccggcgctt ttcaccacgc atggctgggc ccgaacgcga
     1801 gaggagaccg tgctggcgcg cggtctcctc ctggcgaaac ggttggggtc agtcgagctt
     1861 ctcggtgacc ttcttgatgc cgtcggcggc ggtgtcgatc ttgtcgtcga acttgtctgg
     1921 cgccttggcc ttggccatgt cggctgcctt gtcgacaccc tggttgatct gcttcttctt
     1981 acccttgaaa aggcccttga ccttgtccaa caatcccatg tcgttcgtac tccttcgtcg
     2041 attgggtacg ccagtctggc acctgtctcc gccgcttgct tgcaccatcg ggcgcggtga
     2101 taccgtcgcc gcccgtgcct gaactgagcc cggtagcgac gatcttccgg gggaggcgcc
     2161 ggaccgctac ggttgtgcgg cgatgagacc gtcggacgac atcggtggcg agccccgcaa
     2221 agccgaacgt ctccaacaga acgctcacga atgggacaag ggcaaggcgg gcgagcggat
     2281 ggtcgcggcc gcgctcgacg ccctgccgca cgactaccgc gtctatcacc acgtcggcct
     2341 gcccggcacc aaagaggcgc tcgaccacgt ggtggtcggg ccgtgtggcg tcgtggccat
     2401 caacgacacc tatgtcgacg gcgacctgca gcacggcagc ggcacgctgt ggcggggcaa
     2461 ggtgccgatc cggcgcgaga ccgagaatgt gcgctgggaa gccgacgtgt tgtcgaccgc
     2521 gctgcaacag cccgtcgacg cggtgctgtg ctgcgccgaa gccacgctgc ccgaaccggt
     2581 cgtcaccctc gaccgcaccg ccgtcgtcag cctcgacgcg ttgaccgggc acatcatcac
     2641 caaacccgcc aagttcgatg cggacgcgat cgccgtcgtc gccnnnnnnn nnnnnncgtg
     2701 gccccgtggg tgcgcaccgc caccgcccgc ggtgtcgtct cggtgctgac cacccccgac
     2761 gtgacgaccg gttccgaacg cccgggcgcg ttgcctcggc gcgagattcg cacgccgcgg
     2821 cgcacccgca gccagatccg ccaagagcgc gcggcccaac ggttcaccgg catcgagcag
     2881 ccgccgcgcg agaagggcaa ctacggcgtg gccgtgtcgg ccctgttcgc gatcgccgtg
     2941 ctgttcggcg tgtgggcgct gtggagtcgc ctcggcgacg gcacccagcg tgccgccagc
     3001 cccaccacaa ccgccacgcc cacagtgcca ccgatcatca ccgacgccgc gaccaccacg
     3061 acggcggccg cgaccaccac gacggccccc gccgcctcgg tgccgctgcc acccaacgtc
     3121 gagttcgact gccccagccc cggcaagggt tggcaggcga cgctgatcgc gagcgagttc
     3181 ctgcaagacc cgcaggggta ccacgtgtgg tactcggtgc ggctgtcgcc actgacgtac
     3241 tggggactgt tcaagtcggg cctgcgcaca cccgagccga tcgggccggt gtnnnnnnnn
     3301 nngcgatcca gtgcgccgat accggcgcgc cgcgggcgag cagttcctgt tgaagatgga
     3361 ccgtgagcag agcgccgacc ccgagttggc cccgatcgag cgcttcgtca acgcccccga
     3421 cgagacctgc tgaacccgaa aacgcaacaa gcccgccccg aagggcgggc ttgtgcagaa
     3481 agcgaactca ccagcgccaa agactcacca gcgccaaaga ctcaccagcg atagtgagcg
     3541 aaggcgcggt tcgactcggc catcttctgc atgtcgtcgc ggcgcttgac cgcggcaccc
     3601 aggccgttgg cagcatcgag caactcgccg gcgaggcact cggccatcgt cttctcacgc
     3661 cgcttgcggc tgaagtcgac cagccagcgg atggccaacg tgttggcccg cggcgcacgc
     3721 acttcgaccg gcacctggta ggtggcgcca ccgacgcgac ggctgcggac ttcgagcggc
     3781 ggcttgatgt tgtcgacggc ccgcttgagc gtggcgatcg gctcgctgcc ggtcttgtct
     3841 tcgacgatct tcagcgcgtc gtagacgatc ttttcggcaa tgctgcgctt gccgtgcagc
     3901 atgaccttgt tggtgatctg cgagacgagc aacgaccggt agatcgggtc gggcatgatc
     3961 ttgcggcgct cggcgggtcc tttacgtggc atgacttctg gttcctctta ctgccggatc
     4021 acttgggggt cttggcgccg tagcggctac ggctctgctt gcggttcttc accccggccg
     4081 cgtcgagcgc gccacggatg atcttgtaac gcacacccgg caggtcgcgc acacggccac
     4141 cacgcaccag cacgatgctg tgctcttgca ggttgtggcc ctcgcccggg atgtaggcgg
     4201 tgatctcgac gccgctggac aggcgcacac gggcaacctt gcgcagggcc gagttcggct
     4261 tcttgggggt gttggtgtac acgcgagtgc acacgccgcg tcgctgcggc gagcccttca
     4321 gcgcgggcgt cttggtcttg ctgaacttgc tgctgcgccc ttggcgaacc agctgctgaa
     4381 tagtgggcac gcgtctacct ttcgcgttct cagaacgact tccatgcacg cagacggacg
     4441 acccgtcgcg caggaccctg caatgttatg gcgggccgtg ggtactacca acccgcaccc
     4501 agcgcgaagt ggggaaagac gttagcttcc ccacttcgcg tggtcgcgga tatgaaccga
     4561 tcnnnnnnnn nnnnnncctg ctgggtgccg tgcaggttgg ccaggtaggc cgacgcgtcg
     4621 tcgatgtcgc tggagtagaa ctccatcggg cggtgatcgg gagccgagac ctcgaagtcg
     4681 cggtagcgct ccatgccggt gccggccggg atcagcttgc cgatgatgat gttctccttg
     4741 agaccgagca ggccgtcgct gcggccgtcg atggccgcct cggtcagcac gcgggtggtc
     4801 tcctggaacg acgcggccga cagccacgac tcggtggcca gcgacgcctt ggtgatgccc
     4861 atcagctcgg gacggccctc ggcaggacgc tgaccctctt cgaccatccg cttgttggtg
     4921 tcgcggaaga tcttggcgtc g
//
LOCUS       DAORNF010000161         4929 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_12738_length_4929_cov_1.443578, whole genome shotgun sequence.
ACCESSION   DAORNF010000161 DAORNF010000000
VERSION     DAORNF010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4929
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12738_length_4929_cov_1.443578"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..609
                     /locus_tag="PLV68_07730"
     CDS             <1..609
                     /locus_tag="PLV68_07730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HRE01176.1"
                     /translation="RINVAYQDGPATLVRTVQDLGIPVHHYVEVDFQGFKQLVDAIGG
                     VRIGFWKPARDEHTGLYIAESGYHLLDGVQALAYARSRYYENYEDGKWVIDPRSDLGR
                     VERQQHFVNEALKQAIEKVKANPFTAGEVLRSMTTAIRLDEDLDVFTAATTLRQAVDA
                     GVGTYTPAVYGKTVNGNAVLLLGDSAANLFAYFAGDAPAPQS"
     gene            665..1744
                     /locus_tag="PLV68_07735"
     CDS             665..1744
                     /locus_tag="PLV68_07735"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="HRE01177.1"
                     /translation="MKALILAGGAGTRLRPITHTSAKQLVPVANKPILFYGIEAMAAA
                     GISEIGIIVGDTRAEVMETVGDGSRFGVQVTYIPQDAPLGLAHCVLIAQGFLGGDDFV
                     MYLGDNLLEQDLAAFVRAFEAARTSAEPPTAQILLKQVPDPHRFGIATLDEAGHVVHL
                     VEKPADPPSDLALVGVYLFDKTIHTAVASIQPSPRGELEITDAIQWLVDQGHRVRCEL
                     LSGWWIDTGKLTPLLEANRLLLEKLDQRIDGSVDEASAIDGRVVIEAGAVVTNSTIRG
                     PVAIGARATITDSFIGPFSAIGEDCVVANSEVEHSVVMARSTITDIPRLEDSLIGKDA
                     VVARTQQRPRATRLMVGDHCQIDVE"
     gene            1753..>1897
                     /locus_tag="PLV68_07740"
     CDS             1753..>1897
                     /locus_tag="PLV68_07740"
                     /inference="COORDINATES: protein motif:HMM:NF013103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HRE01178.1"
                     /translation="MPNVYESDVVAGAYIVEPTIHGDQRGLFIETYRREWFPNGREMI
                     QGNR"
     assembly_gap    1898..1918
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1919..2312
                     /locus_tag="PLV68_07745"
     CDS             <1919..2312
                     /locus_tag="PLV68_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HRE01179.1"
                     /translation="GAVVGLHYHLHQADYWYVPFGTARVVLHDLREGGPTDGATLTLE
                     LSGDNHMGVFIPPGVAHGFAAVTDMVITYLVDGYYNPDDELGLAWDDPAVVADWGVTD
                     PILSARDQANPGRDALPAGRRPYWKMRT"
     gene            2309..>3258
                     /gene="rfbB"
                     /locus_tag="PLV68_07750"
     CDS             2309..>3258
                     /gene="rfbB"
                     /locus_tag="PLV68_07750"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HRE01180.1"
                     /translation="MKQFVTGAAGFIGSNYVRHVLASTDDEVTVYDALTYAGNLESIR
                     DLLDDRRCRFVHGDICDQDLVTTSMAGHDVVVHFAAETHVDRSIKDGHSFVRTNCFGT
                     NVLCDVARQYDIERFVHISTDEVYGSIEDGSFTEDDRLGPRSPYSAAKAGSDLIALSY
                     FTTHGLPVIVTRCSNNFGPYQFPEKLIPLFTTNLLDGHNVPLYGDGGNVRDWIHVHDH
                     NTAVLAILAKGTVGEIYNIGAGNEVTNREITYRLLELCGRDETFITPVADRLGHDRRY
                     SITHDKTSALGWSTTHTLDQALADTVEWYRNNRAWWEPLKA"
     assembly_gap    3259..3321
                     /estimated_length=63
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3322..>3810
                     /locus_tag="PLV68_07755"
     CDS             <3322..>3810
                     /locus_tag="PLV68_07755"
                     /inference="COORDINATES: protein motif:HMM:TIGR01214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HRE01181.1"
                     /translation="LGRDVELHCLAAGDEVVALGHGALDVTDRDAVLGVVTTVRPDAV
                     LHCAAWTAVDACEADPGRAFAANALAVRWVAEGCDRVDAHLVTISTDYVFDGSKTGPY
                     HEWDQPSPRSVYGASKLAGEAEAAALGSAAAVVRTSWVCGEHGANMVKTILRLAAERD
                     QL"
     assembly_gap    3811..3888
                     /estimated_length=78
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3889..4142
                     /locus_tag="PLV68_07760"
     CDS             <3889..4142
                     /locus_tag="PLV68_07760"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HRE01182.1"
                     /translation="SGVHHLTNQGAVSWYEFAQTVVAAAGRSPGMVSPIETAALQPPR
                     PAPRPANSVLDNAVWRASGLEPLRDFRAPLTELVARLST"
     gene            complement(4132..>4929)
                     /locus_tag="PLV68_07765"
     CDS             complement(4132..>4929)
                     /locus_tag="PLV68_07765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01183.1"
                     /translation="WTLLVFPNTFEYFFIAYEAIRTRWDPRRLSAHRLVGLAAAIWNV
                     VKLPQEWWIHIARLDATDEISAHPWIWLVLLGAAATLTGAVWSRRDRIPAADWSFTTR
                     VDAHLPRFDPDAGPWQRFFTMVLVEKVVLLTLICVIFAQVLPDVGSTNLQLAVGVAVL
                     VVANAAISQSMRHRGRSWPNIISQFGAMLTVNMGIVSVDAVLSPRGGDNTPELSTVFF
                     MVLLAMLIALFDRYRATRNKIDKLPGAITVWKRERAGRGTPQAALRY"
BASE COUNT          823 a         1640 c         1560 g          744 t
ORIGIN      
        1 cgcatcaacg tcgcctacca ggacggcccg gccacgctgg tgcgcacggt gcaggacctg
       61 ggcattcccg tgcaccacta cgtcgaggtc gacttccagg gcttcaagca gctcgtcgat
      121 gcgatcggtg gcgtgcggat cgggttctgg aaacccgctc gtgacgagca caccggcctg
      181 tacatcgccg agagcgggta tcacctgctc gacggcgtgc aagccctcgc gtatgcccgc
      241 agccgctact acgagaacta cgaggacggc aagtgggtga tcgacccccg ctccgatctg
      301 ggccgggtcg agcgccaaca gcacttcgtc aacgaggcgc tgaagcaggc gatcgagaag
      361 gtcaaggcga atcccttcac cgccggcgag gtgttgcgtt cgatgaccac cgcgatccgc
      421 ctcgacgagg acctcgacgt gttcaccgcc gccaccaccc tgcgccaggc ggtcgatgcc
      481 ggtgtcggca cctacacgcc cgccgtgtac ggcaagacgg tcaacggcaa tgcggtgctg
      541 ctgctgggcg acagtgcggc aaacctgttc gcctacttcg cgggcgacgc gcctgcgcct
      601 caatcgtgat cgcagcgttc gtgctcgctg ctgtgacgca cggagccatt agcctgaccg
      661 cctcatgaaa gcgttgatcc tggccggcgg agccggtacc cggttgcgac cgatcaccca
      721 caccagcgcg aagcaattgg tgccggtggc caacaagccg atcctgttct acggcatcga
      781 ggcaatggct gcagccggca tctccgagat cggcatcatc gtcggcgaca cccgcgccga
      841 ggtgatggag acggtcggtg acggaagccg gttcggcgtg caggtcacct acatcccgca
      901 agacgccccg ctgggcctgg cccactgcgt gctgatcgcg cagggcttcc tcggcggcga
      961 cgacttcgtc atgtacctgg gcgacaacct gttggaacag gacctcgccg ccttcgtgcg
     1021 agccttcgaa gcggcccgta ccagcgccga gccgccgacg gcgcagatcc tgctgaagca
     1081 ggttcccgac ccgcaccgct tcggcatcgc caccctcgac gaggcaggcc acgtcgtgca
     1141 cctggtcgaa aagccggccg acccgccgtc ggacctggcc cttgtcggcg tctacctgtt
     1201 cgacaagacg atccacaccg cggtcgcgtc gatccagccg tcgccccgcg gcgagctcga
     1261 gatcaccgac gcgatccagt ggctggtcga ccagggccac cgcgtccgct gcgaactgct
     1321 gtcgggctgg tggatcgata ccggcaagct gaccccgctg ttggaggcga accggctgct
     1381 gttggagaag ctcgatcagc gcatcgacgg cagcgtcgac gaagcgtcgg cgatcgacgg
     1441 tcgggtcgtg atcgaggccg gagcggtcgt gaccaactcc accatccgtg ggcccgtggc
     1501 catcggggcg agagccacca tcaccgacag cttcatcggg ccgttctcgg cgatcggcga
     1561 ggattgcgtc gtggccaaca gtgaggtcga gcactcggtg gtgatggccc gctccaccat
     1621 caccgacatc cctcgcctgg aggacagcct gatcggcaag gatgccgtcg tcgcccgcac
     1681 ccaacagcga ccccgcgcga cgcggctgat ggtcggtgac cactgtcaga tcgatgtcga
     1741 ataggagcag cgatgccgaa cgtgtatgaa tcagacgttg ttgcaggtgc atacatcgtc
     1801 gagccgacga ttcacggcga tcagcgcggg ctgttcatcg agacgtatcg ccgcgagtgg
     1861 tttcccaacg gtcgggagat gatccaaggc aaccgcgnnn nnnnnnnnnn nnnnnnnngg
     1921 gggccgtcgt cgggttgcac taccacctcc accaggccga ctactggtac gtgcccttcg
     1981 gcacggcgcg ggtcgtgctc cacgatctcc gcgagggcgg accgaccgac ggcgcgacgc
     2041 tgaccctgga gctgtcgggt gacaaccaca tgggcgtgtt catcccgccg ggtgtggccc
     2101 acgggttcgc ggcggtcacc gacatggtca tcacctatct ggtcgacggc tactacaacc
     2161 ccgacgacga gctcggcctg gcctgggacg accccgcggt ggtggccgac tggggcgtga
     2221 ccgatccgat cctgtctgcc cgcgaccagg ccaaccccgg ccgtgatgcg ctgccggccg
     2281 gccgccgtcc gtactggaag atgaggacat gaagcagttc gtcaccggcg cggccgggtt
     2341 catcggttcg aactacgtgc gccacgtgtt ggccagcacc gacgacgagg tcacggtcta
     2401 cgacgcgttg acctacgcgg gcaacctcga gtcgatccgc gatctgctcg acgaccgccg
     2461 ctgccggttc gtccacggcg atatctgtga tcaggacctg gtcaccacgt cgatggccgg
     2521 tcacgacgtg gtcgtccact tcgcggccga gacccacgtc gaccgttcga tcaaggatgg
     2581 ccacagcttc gtgcgcacca actgcttcgg caccaacgtg ttgtgcgacg tcgcccgcca
     2641 gtacgacatc gagcggttcg tccacatctc caccgacgag gtctacggca gcatcgagga
     2701 cggttcgttc accgaggacg atcgccttgg gccgcgttcg ccgtattcgg cggccaaggc
     2761 cgggtccgat ctgatcgcgt tgtcgtactt caccacccac ggtctgccgg tgatcgtcac
     2821 ccgctgctcg aacaacttcg ggccgtacca gttccccgag aagctgatcc cgctgttcac
     2881 caccaacctg ctcgacggcc acaacgtgcc gttgtacggc gacggtggca acgtgcgcga
     2941 ctggatccac gtgcacgacc acaacaccgc ggtgctggcc atcttggcca agggaacggt
     3001 gggggagatc tacaacatcg gtgccggtaa cgaggtcacc aaccgcgaga tcacctaccg
     3061 cctgctcgag ctgtgcgggc gcgacgagac cttcatcacg ccggtcgccg accgcctcgg
     3121 ccacgatcgt cggtacagca tcacccacga caagaccagt gcgctggggt ggtccaccac
     3181 ccacaccctc gaccaggccc tcgccgacac ggtcgagtgg taccgcaaca accgcgcctg
     3241 gtgggagccg ctcaaggcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nagctcggcc gcgacgtcga gttgcactgc ctggctgctg
     3361 gtgacgaggt ggtcgcgctc ggccacggcg cactcgacgt caccgaccgc gacgcggtgc
     3421 tgggtgtcgt caccacggtg cgccccgacg ccgtgctgca ctgcgcggcg tggaccgcgg
     3481 tcgacgcgtg cgaggccgac ccgggccggg cctttgccgc caacgccctc gccgtgcgct
     3541 gggtggccga aggatgcgac cgcgtcgacg cgcacctggt gacgatcagc accgactacg
     3601 tcttcgacgg cagcaagacc gggccgtatc acgaatggga tcagccgagc ccgcgatcgg
     3661 tgtatggcgc gtcgaagctg gccggcgaag ccgaagcagc ggccctgggc agcgctgccg
     3721 cagtggtgcg caccagttgg gtgtgcggcg agcacggcgc caacatggtc aagaccatcc
     3781 ttcgcctggc ggccgaacgc gaccagttgg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngc agcggcgtgc
     3901 atcacctgac caatcagggc gcagtcagtt ggtacgagtt cgcccaaacc gtggttgctg
     3961 cagcggggcg ttcgccgggc atggtcagcc ccatcgagac cgccgcgctg caaccaccgc
     4021 gccccgcgcc gcggccggcc aacagcgtgt tggacaacgc ggtctggcgc gccagcgggc
     4081 tcgagcccct gcgcgacttc cgcgcaccac tcaccgaact ggtcgcccgc ctcagtacct
     4141 gagcgccgcc tgcggggtgc ctcgcccggc acgctcgcgc ttccagacgg tgatcgcgcc
     4201 gggcagcttg tcgatcttgt tgcgggtggc ccggtagcgg tcgaacaggg cgatcagcat
     4261 cgccagcaac accatgaaga acacggtgct caactcgggc gtgttgtcac cgccgcgcgg
     4321 tgacaacacc gcgtcgaccg agacgatgcc catgttcacc gtcagcatcg caccgaactg
     4381 gctgatgatg ttcggccacg agcggccgcg atggcgcatc gactggctga tcgccgcgtt
     4441 ggcgacgacg agcacggcga caccgacggc cagttggagg ttggtcgaac cgacgtcggg
     4501 cagcacctgg gcgaagatca cgcagatcag cgtcagcagc accacctttt cgaccagcac
     4561 catcgtgaag aaccgctgcc atggcccggc gtcaggatcg aagcggggga ggtgggcgtc
     4621 gacccgcgtc gtgaacgacc agtccgctgc cgggatgcgg tcacggcgcg accacaccgc
     4681 accggtcagg gttgccgctg cgcccaacag caccagccag atccaggggt gcgcgctgat
     4741 ctcgtcggtg gcgtcgagcc gggcgatgtg gatccaccat tcctggggca gcttgaccac
     4801 gttccagatc gccgctgcca ggccgaccag tcggtgggcg gacagccgcc gcgggtccca
     4861 gcgggtgcgg atcgcctcgt acgcgatgaa gaagtactcg aacgtgttag ggaagaccag
     4921 caacgtcca
//
LOCUS       DAORNF010000162         4918 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_12774_length_4918_cov_1.769073, whole genome shotgun sequence.
ACCESSION   DAORNF010000162 DAORNF010000000
VERSION     DAORNF010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4918
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12774_length_4918_cov_1.769073"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1560
                     /locus_tag="PLV68_07770"
     CDS             <1..1560
                     /locus_tag="PLV68_07770"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRE01184.1"
                     /translation="GGDVTNPPTTNGETTTTATPVTDPPSGIVTGGSLTVGMDGDTAC
                     GFIPSLCSVSYGPGATRFAVVENLVRPANNSDGYEFQLAESVEKNDDSTVFTFTLRSD
                     VKWSDGTPFTAGDVKTLFDDYVMADGSFVRGNVALVTSTEAPDDHTVVFTLAAPQAPF
                     PVLLTNVPIWKPEPGIEATTLPIGTGPFVFDSFEANVKTVLKKNPNYYRQDANGVQLP
                     YLDEIVIVPITSGDTRVNSLESGDIDIAMSVDPLIARRLQDASTVYKVELNAGNGLFF
                     NNSSGPTSDIRVRKALAFATLKDDVLAAIGMDTPRDEYYVPESAWYSQEASAATPGYD
                     PDEAKALLEEYKADPARSDGKAAGSPVTIDISHVQGAVTGESIAAVAQQQWQDVGFEV
                     TVTPKDQSTLIGDAISGNFNVNYFGWATPHPYNLLVRNYGHWPETQSNYTHFNSDELI
                     EIVQKMSVAKTAAEMDELIQESNIILAEGVPLIFLHSTPLVWGTNDAVGNVELLPGNG
                     FIEWPTLSVAG"
     assembly_gap    1582..1613
                     /estimated_length=32
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1701..2654
                     /locus_tag="PLV68_07775"
     CDS             1701..2654
                     /locus_tag="PLV68_07775"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRE01185.1"
                     /translation="MSTSVLRKRIIRLIVTLLAVSFLSFLLTSLLPGDPATTILGAEN
                     ISKEAVDCDHIGHVNAGCYVRWLGDAVTGDLGQSYTLSRPVSDLIGARLPITLELVAV
                     SLTIALLVSIPLGIYTAYKPNSPLAKFSSLITYAILSIPSFVIGILLILFFAIKNDFL
                     PAAGWVPLSESVPQNLRRIILPAITLALPQIAVLSRLLRGDMMATLDQDFVSFADSKG
                     LSTRRILLGHAFRPSSFSLLTMAGTSVGYLMGGTLIVETQFNLPGMGTLALGAINSRD
                     LVTVQGVTVFVATVFVVINFLVDVLYAVLDPRIRRNHGAAA"
     gene            2638..2952
                     /locus_tag="PLV68_07780"
     CDS             2638..2952
                     /locus_tag="PLV68_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01186.1"
                     /translation="MALQLDLETPDGSPQETARAEQEPFDGPPRRRRLAWLQRIPLSF
                     CLVWLALVVFAAISASWLPWVQDPNKVKPVDRLQGPSIKHWFGTDQLGRDTFARAARG
                     SR"
     gene            2970..>3455
                     /locus_tag="PLV68_07785"
     CDS             2970..>3455
                     /locus_tag="PLV68_07785"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRE01187.1"
                     /translation="MAIGLTLGSLLGIVAGYMRGRVDGFVSWLIDVILSFPSLVLLIA
                     LVAFTGGGLRDITLALAFLAIPIYARLARAHSLSVGGEEYVLAARAGGSGRLRILWRE
                     IVPNVLPSIMAYALVAMSLAIVTEGSLSYLGLSVKVPQASWGGQIANNQNFLRDTAWG
                     VI"
     assembly_gap    3456..3613
                     /estimated_length=158
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3614..>4072
                     /locus_tag="PLV68_07790"
     CDS             <3614..>4072
                     /locus_tag="PLV68_07790"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRE01188.1"
                     /translation="LHFRTRHGNIRAVDGVSFDVFKGQTLGIVGESGSGKTVLSRSIM
                     GLLYGKNVIRRGRVVFDGRDISKVTRKQGRALRGTEVAMVFQDPMTSLNPVMKIGRQL
                     TEGMEAHLDLSKEQRYAAALDLMRSVGIPSPEQRLEQYPFELSGGMRQRV"
     assembly_gap    4073..4126
                     /estimated_length=54
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4127..>4568
                     /locus_tag="PLV68_07795"
     CDS             <4127..>4568
                     /locus_tag="PLV68_07795"
                     /inference="COORDINATES: protein motif:HMM:TIGR01727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRE01189.1"
                     /translation="TALDVTIQAQILNLLEAKKRERNMAMILVTHDLGVVAGRTDEIM
                     VMYAGKVVERAPTAELFSNMRMPYTEALLNSIPKLDEPSHTPLITIGGRPPLMTAPPQ
                     GCRFSPRCPYAQRKCTEEEPPLMTDSSVSSNHVYACWFPVGITSR"
     assembly_gap    4569..4628
                     /estimated_length=60
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4629..>4918
                     /locus_tag="PLV68_07800"
     CDS             <4629..>4918
                     /locus_tag="PLV68_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004118222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRE01190.1"
                     /translation="VVVTGDERMPLLSVRDLTVEFATAKQKIHAVSGVSFDIFEGETL
                     GLVGESGCGKSTTSRAIMQLPRPTSGSVLFEGQDMTTLSPADLRKTRTRLQM"
BASE COUNT          785 a         1465 c         1468 g          896 t
ORIGIN      
        1 ggcggggacg tgacgaaccc gccgacgacc aatggtgaga cgaccacgac ggcgactccg
       61 gtcactgatc ccccgagtgg gatcgtcacc ggcggttcgc tcacggtcgg catggatggc
      121 gacacggcct gcggtttcat tccgtcgctg tgctcggtgt cgtacggccc cggagcaact
      181 cgcttcgcgg tcgtcgagaa cctggtgcgt cctgcgaaca actccgacgg ctacgagttc
      241 cagttggccg agtcggtcga gaagaatgac gactcgaccg tcttcacctt cacgctgaga
      301 agcgatgtga agtggagtga cggcacgccg ttcactgcgg gcgacgtgaa gacgttgttc
      361 gacgactacg tcatggccga cggttccttc gtccgaggca acgtggcgct ggtcaccagc
      421 accgaggcgc ccgacgatca cacggtggtc ttcacgctgg cggcgccgca ggctccattc
      481 ccggtgctgc tcaccaacgt gccgatctgg aagcctgaac ccggcatcga ggcgacgaca
      541 ctgccgatcg gcaccggtcc gttcgtattc gacagcttcg aggccaacgt caagacggtg
      601 ctgaagaaga acccgaacta ctaccgccag gatgccaacg gcgttcagtt gccgtacctc
      661 gacgagatcg tgatcgtgcc gatcacgtct ggcgataccc gggtgaactc cttggagtcg
      721 ggtgacatcg acatcgcgat gtcggtcgat ccgctcatcg cgcggcggtt gcaggacgcc
      781 tcgaccgtct acaaggtcga gctgaacgca ggtaacggtc tcttcttcaa caacagctcg
      841 ggtccgacct cggacatccg ggtgcgcaag gcgctcgcct ttgccacgct caaggacgac
      901 gtcctcgcgg cgatcggtat ggacacgcct cgcgacgagt actacgttcc cgagagcgcg
      961 tggtactcgc aggaggcctc tgcggcaacg ccgggttatg accccgacga ggccaaggcg
     1021 ctgttggagg agtacaaggc cgacccggcc cgttccgacg gcaaggcagc gggttcgccg
     1081 gtcaccatcg acatctcgca cgtgcagggt gcagtgaccg gcgagagcat cgccgctgtc
     1141 gcccagcagc agtggcaaga cgtcggcttc gaggtgacgg tcacgccgaa ggaccagtcg
     1201 acattgatcg gtgacgccat ctccggcaac ttcaacgtca actacttcgg ctgggcgacg
     1261 ccgcacccgt acaacttgct ggtgcgcaac tacggtcact ggcccgagac gcagtccaac
     1321 tacacgcact tcaacagcga cgagctgatc gagatcgtgc agaagatgtc ggtggccaag
     1381 accgctgccg agatggacga gctcatccag gagtcgaaca tcatcctcgc tgaaggagtg
     1441 ccgctcatct tcctgcacag cacgccgctg gtgtggggaa ccaacgatgc cgtcgggaac
     1501 gtggaactgc tcccgggcaa cggcttcatc gagtggccta cactcagcgt ggctggctga
     1561 tcggtcgttg acgacctgtc gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngattgcc
     1621 attggggaac gaagtcgagt gtccatgagg acggacgcca tcagagtcga tgaaggtgag
     1681 gggactcgtt gagtgcaacc gtgagcacgt cggtgcttcg caaacggatc atccgcttga
     1741 tcgtcacgct cctagctgtt tcgttcttgt cgttcttgtt gacgtcactc ctgccgggtg
     1801 acccggcgac gacgatcctg ggagcggaga acatcagcaa agaggccgtc gattgcgacc
     1861 acatcggcca cgtcaacgcc ggctgctacg tgcgctggct gggcgatgcc gtgaccggtg
     1921 atctcgggca gtcgtacacg ctctcccgcc cggtgagcga cctgatcggg gcccggcttc
     1981 cgatcacact cgagctggtt gcggtctcgc tcacgattgc cctcctggtc tcgatccctc
     2041 tgggcatcta caccgcctac aagccgaact ccccgctcgc gaagttctcg tcgttgatca
     2101 cctatgcgat cttgtcgatc ccgtcgttcg tcatcggcat cttgttgatc ttgttctttg
     2161 cgatcaagaa cgatttctta cctgcggccg gctgggtgcc cctcagcgag agcgtgccgc
     2221 agaacttgcg gcgaatcatt ctccctgcga tcacgctcgc gctgccgcag attgccgtgc
     2281 tctcccgcct gctccgtggc gacatgatgg cgacgctcga ccaggacttc gtctcgttcg
     2341 ccgattccaa gggtctctcc acaaggcgca tcctgctggg gcacgcattc cggccgtcgt
     2401 cgttctcgct gttgacgatg gccggcacgt cggtgggcta cctgatgggt ggcacattga
     2461 tcgtggagac ccagttcaac ctgcccggaa tgggcacgct cgcgctgggc gcgatcaact
     2521 cgcgcgacct ggtcaccgtc cagggggtca cggtcttcgt ggccacagtg ttcgtcgtca
     2581 tcaacttcct cgtcgatgtc ttgtacgcag tgctcgaccc gagaatcagg cggaaccatg
     2641 gcgctgcagc ttgacctcga gactccggac ggttcgccgc aggagacagc ccgcgccgag
     2701 caggagccgt tcgacggccc gccgcgccgt cgccgcctcg cctggctcca gcggatcccc
     2761 ctgtcgttct gtctcgtctg gctcgcgctg gtcgtgttcg cggcgatttc ggccagttgg
     2821 ctgccctggg tgcaggaccc gaacaaggtc aagccggtcg atcgcttgca gggtccgagt
     2881 atcaagcact ggttcggcac cgatcagctc ggacgcgaca cgttcgcccg tgcggctcgc
     2941 gggtctcgct gatcatcagc gtcttttcgg tggcgatcgg cctcacactc ggcagcttgc
     3001 tggggatcgt cgctggctac atgcggggcc gtgtcgacgg gttcgtctcg tggctgatcg
     3061 acgtcatcct gtccttcccg tccttggtcc tcttgatcgc tctggtggct ttcaccggtg
     3121 gtggcctgcg tgacatcacc ctggctctcg cctttctcgc catcccgatc tatgcccgcc
     3181 tcgcccgggc tcattcgttg tcggtcggtg gagaggagta cgtgctcgct gcccgggccg
     3241 gtggctcggg acggctgcgc atcttgtggc gtgagatcgt tcccaacgtg ttgccctcga
     3301 tcatggcgta tgcgctggtc gcgatgagcc tcgcgatcgt caccgaaggc agcttgagct
     3361 acctcggcct gagcgtgaaa gtaccgcaag cgtcgtgggg tgggcagatc gccaacaacc
     3421 agaactttct acgggacacg gcatggggcg tgatcnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnncgctgca cttccgcacg cgacacggca acatccgcgc ggtcgatggc
     3661 gtgtcgttcg acgtattcaa gggccagacg ttggggatcg tgggcgagtc cggttccggc
     3721 aagactgtgc tgtcgcgttc gatcatgggt ctgttgtacg gcaagaacgt gatccgccgc
     3781 ggtcgggtcg tgttcgacgg tcgggacatc tccaaggtca cgcgtaagca aggccgcgcc
     3841 ttgcgtggca ccgaggtcgc gatggtcttt caagatccga tgacgtcgtt gaacccggtg
     3901 atgaagatcg gccgtcagtt gaccgagggc atggaggccc acctcgacct ctccaaggag
     3961 cagcggtacg ccgctgccct cgacctgatg cgcagcgtcg ggatcccgtc acccgaacag
     4021 cggctggagc agtatccgtt cgagctgtcg ggcggcatgc gccagcgggt gannnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnacgg cgctcgacgt
     4141 caccatccag gcccagatcc tgaatctgct cgaagccaag aagcgcgagc gcaacatggc
     4201 gatgatcttg gtcacgcacg acctcggggt cgtcgctggt cgcacggacg agatcatggt
     4261 gatgtacgca ggcaaggtgg tggagcgggc tccgacggcc gagctgttct cgaacatgcg
     4321 catgccgtac accgaagcgc tgctcaactc catcccgaag ttggacgagc cgtcgcacac
     4381 gccgctgatc acgatcggtg gccgtccacc gctgatgacg gcaccgccgc aggggtgccg
     4441 attctcgcca cgctgcccgt acgcgcaacg caagtgcacc gaagaggagc cacccctgat
     4501 gaccgactcg tcggtgtcct ccaaccacgt gtatgcctgc tggttccctg ttggcatcac
     4561 ctcgcgtcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4621 nnnnnnnngt tgtcgtgacc ggtgacgagc ggatgccgtt gttgtcggtc agggacctga
     4681 cggtcgagtt cgcgacggcg aagcagaaga tccacgccgt gtcgggggtg agcttcgaca
     4741 tcttcgaagg cgagacgctc ggcctggtcg gtgagtcggg gtgcggcaag tcgaccacgt
     4801 cgcgagcgat catgcagctg ccgcgtccga cctctgggtc ggtgctgttc gaaggtcagg
     4861 acatgaccac gctgagcccc gcggacctgc gcaagacccg cacccggttg cagatgat
//
LOCUS       DAORNF010000163         4904 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_12829_length_4904_cov_1.844092, whole genome shotgun sequence.
ACCESSION   DAORNF010000163 DAORNF010000000
VERSION     DAORNF010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4904
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12829_length_4904_cov_1.844092"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1456
                     /locus_tag="PLV68_07805"
     CDS             <1..>1456
                     /locus_tag="PLV68_07805"
                     /inference="COORDINATES: protein motif:HMM:NF012573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01191.1"
                     /translation="IYAGRGNDYVDGGAGDDVIHGRAGNDTLIGGTGNDTFVVRFTDA
                     GTTTIIDDGGTLFHGTFKPANAPSSWATSGYEIKGDATVVSAGVWDLAVTDNSNVVQH
                     LNLAWTGGDLTITKSGGSQTVVVKNYVNGTFGITLENSPPTNLALSGSTVLANVPTVF
                     LSEGLAVGSVIGMVSAIDFDNDALTYSLDNDAGGKFELVGTELRLKAALDYETATSHT
                     VTLKVTDSEGNVSTKDFAVEVRDVLNPQIDQVVISELSMHRVGFDQVLAAVTTLVEST
                     STGQVPAGAGPTASAPTGSLPTESVQPEGSSPDPLALPEPLKVPEPLKVPEPLKVMVI
                     GDSTSLGAAVGLAHAADHRFEVLWAGRQNCPVVAVAEIKYRLGAAFSAADCPSATGEW
                     EQSIVTFGPDAIVVVASVTEQVPQRYPGETEWVTPGDATYVAYHREALDDLVALAAPV
                     GAVVVVADAPPSTRSKAWVSNDAIAAWNATVADW"
     assembly_gap    1457..1592
                     /estimated_length=136
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1822..>2292
                     /locus_tag="PLV68_07810"
     CDS             1822..>2292
                     /locus_tag="PLV68_07810"
                     /inference="COORDINATES: protein motif:HMM:NF018590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HRE01192.1"
                     /translation="MAADDKAPQTIVGISFADAFRAQEFLTATTRLAANKSLVLHDAV
                     IVRKDPDGRTHVVETIDPSPGRSATSGALWAGFLGLLVGGPVGWIAGGVIGAGAGAAA
                     AKVVDLGVPDEWVRWFKDAVEPGTTTVILLLTDVVVNHLVAEAERFVGATLVYSN"
     assembly_gap    2293..2406
                     /estimated_length=114
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2607..>4522
                     /locus_tag="PLV68_07815"
     CDS             2607..>4522
                     /locus_tag="PLV68_07815"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HRE01193.1"
                     /translation="MAEAIRVSGPVAGTDKTLSFETGKLAQQSQGAVVATIGRTTVLA
                     TANAAKDVRPGIDFFPLTVDVEERAYAAGKIPGSFFRREGRPTENAILTCRLTDRPLR
                     PCFPDGFRNEVQVVLNILGADQENPHDILAINAASAALVISGIPFDGPIGAVRIAYSQ
                     DGEWIPYPTFAEGEASTFELVVAGRLLGDDDVAIMMVEAGGTEKSFAYYADGAPKVDE
                     AVLANGLEACKVWIRDSIKLQQQLLASVIATKGPITPLPFSAQEDYSPEIADAVAEAV
                     RDQLAHAVTISAKADRNAATDAAADAAIAALAGEGGRFAGQEKAIKEAVRSLTKKLVR
                     KRIVDEGLRIDGRGPRDLRPLSSEVGILPTAHGTGLFQRGETQVMNVATLAMPRMNQL
                     LDTLDPETHKYFLFHYNMAPWANGETGRVGSPKRREIGHGALAGRALTPGHGGVLDRV
                     DALLPVLPSQEEFSYTLRLVAEVLASNGSTSMASVCSGSLALMDAGVPIKAPVAGIAM
                     GLVYAEGKYTTLTDILGAEDAFGDMDFKVAGTADAITALQLDTKIDGIPADVLVAALA
                     QAKEARLQILDNMAACIAAPRDEVNETAPKIVSFNVPLDKVGEIIGPKGKVINTIQQE
                     TGADISVDDDGVHG"
     assembly_gap    4523..4697
                     /estimated_length=175
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4698..>4904
                     /locus_tag="PLV68_07820"
     CDS             <4698..>4904
                     /locus_tag="PLV68_07820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01194.1"
                     /translation="KLGNGKRINAVEDVLTLGDEIEVRVDEIDDKGKVSLSPTSFTAP
                     AGGSDGGEAPRERGPRRERQSDSS"
BASE COUNT          776 a         1492 c         1499 g          712 t
ORIGIN      
        1 tgatctatgc aggccgcggc aacgactatg tcgacggcgg cgccggcgac gacgtcatcc
       61 atggccgggc cggcaacgac acgctgatcg gcggcacggg caacgatacc ttcgtcgtgc
      121 gcttcaccga cgcgggcacc accacgatca tcgacgatgg cggcacgctg ttccatggca
      181 cgttcaagcc cgccaacgcg ccgtcgtcgt gggccaccag cggatatgaa atcaagggcg
      241 acgccactgt ggtcagcgct ggcgtctggg acctggcggt cacggacaac agcaatgtcg
      301 tgcaacacct caaccttgcc tggacgggcg gcgatctgac catcaccaag agcggcggtt
      361 cgcagacggt cgtggtgaag aactacgtca acgggacctt cggcatcacg ctggagaatt
      421 cgccgccgac caatctggcg ctttcgggct ccaccgtcct tgccaatgta ccgaccgttt
      481 tcctgagcga aggtcttgcc gtgggatcgg tgattggcat ggtttcggca attgatttcg
      541 acaatgacgc cctgacctat tcgctcgaca acgacgccgg aggcaagttc gagttggtgg
      601 gcaccgagct caggttgaag gccgccctcg actacgagac ggccaccagc cacacggtca
      661 cgctgaaggt caccgattcc gaaggcaacg tctcgaccaa ggacttcgct gtcgaggttc
      721 gcgacgtgct caatccgcag atcgatcagg tcgtgatcag cgagctgtcg atgcatcgtg
      781 tcggtttcga ccaggtgctg gccgccgtga cgacgctggt cgaatcaacg tcaaccgggc
      841 aggtgccagc aggagcgggg ccgacagcgt cagcgccaac ggggtcattg ccaacggaat
      901 cagtgcaacc ggaaggatcg tcgccggatc cgctggcgct gccggaacca ctgaaggtgc
      961 cggaaccact gaaggtgccg gaaccactca aggtgatggt catcggtgac agtacgtcgc
     1021 tcggtgcggc ggttggcctg gcccatgcgg ccgatcatcg gttcgaggtt ctgtgggccg
     1081 ggcggcagaa ctgtccggtc gtggccgtgg cggagatcaa gtaccgcctg ggcgcggcgt
     1141 tctcggcggc ggactgcccc agcgcgaccg gcgagtggga gcagtcgatc gtcacgttcg
     1201 gtccggacgc gatcgtggtc gtcgccagcg tgacagagca ggtgccgcag cgataccccg
     1261 gcgagaccga atgggtcacc cctggcgacg cgacgtatgt cgcctatcac cgcgaggcgc
     1321 tggacgatct ggtcgcgctg gccgcaccgg tcggtgcggt cgtggtggtg gccgacgctc
     1381 caccgtccac ccgatcgaag gcgtgggtga gcaacgatgc gatcgcggcc tggaacgcga
     1441 ccgtggccga ttgggannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntcccttcg ccgcggcggt cgaggcacgc
     1621 gtcaccgagt tgatcgcggc cgcgaccgcc agtggttgtc gcacggcgac cgacaacggt
     1681 ggctggcgac tcgacctgtc tcgctgttga accgcgctgt cgagacctgc actcgatcgc
     1741 gttcgcggtg atgatcggac cgagcgcgat acggttggcg gctcggaccc gcatccggtc
     1801 acgaccagga aggacctgca catggccgca gacgacaagg caccgcagac gatcgtcggt
     1861 atctcctttg ccgatgcgtt ccgcgcccag gagttcctca ccgccacgac gcggctggcg
     1921 gccaacaagt cgctggtgtt gcacgacgcg gtcatcgtgc gcaaggaccc cgacggacgc
     1981 acccacgtcg tcgagacgat cgatccgtcg cccggccgca gcgccacctc cggtgccctg
     2041 tgggccgggt tcctgggcct gttggtcggc ggaccggtcg gctggatcgc gggaggggtg
     2101 atcggcgccg gagccggtgc cgcggcagcc aaggtggtcg atctgggtgt gcccgacgag
     2161 tgggtgcgct ggttcaagga cgcagtcgag cccggcacga ccacggtgat cttgctgttg
     2221 accgatgtgg tcgtcaacca cctcgtcgcc gaagccgagc gcttcgtcgg tgccacgctg
     2281 gtgtactcca acnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnncaac acgccggtca ctggtaagct gacccgtggt ctggactcgg tccgggctgt
     2461 atcaaaccaa acctgagcgg acgaacagtt cggttgtcag tcgcctttgc cggaaccatc
     2521 gggtgcccgg ccgtgccgac tgggaaccgg ctccgctcca cttgccgagg tgcgagagac
     2581 atcgcgcccg gccgaaagga gctctaatgg ctgaagccat tcgagtgtcg ggccccgttg
     2641 cgggtaccga caaaaccctg tcgttcgaga cgggcaagct ggcccagcag tcccagggtg
     2701 ctgtggtggc caccatcggc cgcaccacgg tgctggcgac ggccaacgcg gccaaggacg
     2761 tgcgtcctgg catcgacttc ttcccgctga cggtcgacgt cgaagaacgt gcctacgccg
     2821 cgggcaagat ccccggctcg ttcttccgcc gtgaaggtcg tccgaccgaa aatgcgatcc
     2881 tcacctgtcg cctcaccgac cgcccgctgc gcccgtgctt tcctgacggg ttccgcaacg
     2941 aggtccaggt ggtgctgaac attctcggtg ccgaccagga gaaccctcac gacatcctcg
     3001 cgatcaacgc cgcgtcggcc gcgctggtca tcagcggcat tcccttcgac ggcccgatcg
     3061 gtgccgtgcg catcgcctac agccaagacg gcgagtggat cccatatccc acctttgccg
     3121 agggtgaggc gtccacgttc gaactggtcg tcgctggtcg cctgctcggc gacgacgacg
     3181 tcgcgatcat gatggtcgaa gccggtggca ccgagaagag cttcgcctac tacgccgacg
     3241 gtgcgccgaa ggtcgacgaa gcggtgctcg ccaacggcct cgaagcctgc aaggtgtgga
     3301 tccgcgactc catcaagctg caacagcagt tgctcgcgag cgtcatcgcc accaagggac
     3361 cgatcacgcc gctgccgttc agcgcgcagg aggactactc gcccgagatc gccgacgccg
     3421 tcgccgaggc ggtgcgcgac cagctcgccc acgccgtcac catcagcgcc aaggccgatc
     3481 gcaacgcggc caccgatgcc gcggccgacg ccgccatcgc cgccctcgcg ggcgagggtg
     3541 gtcgcttcgc cggtcaggag aaggcgatca aggaagcggt gcgcagcctg accaagaagc
     3601 tggtccgcaa gcgcatcgtc gacgagggcc tgcgcatcga cgggcgtggc ccgcgcgatc
     3661 tgcgcccgct gtcgtccgag gtcggcatcc tgcccaccgc acacggcacg ggcctgttcc
     3721 agcgtggcga gacccaggtc atgaacgtcg ccaccttggc gatgccgcgc atgaaccagc
     3781 tgctcgacac gctcgatccc gagacccaca agtacttcct gttccactac aacatggcgc
     3841 cgtgggccaa cggtgagacc ggtcgcgtcg gttcgccgaa gcgccgcgag atcggccacg
     3901 gtgccctcgc cggccgggcg ctgacgcccg gccacggcgg cgtgctcgac cgtgtggatg
     3961 cgctgctgcc cgtgctgccg agccaggagg agttcagcta cacgctgcgc ctcgtggccg
     4021 aggtgctggc gtccaacggg tcgacgtcga tggcgtcggt ctgctcgggc agcctggcgt
     4081 tgatggacgc cggcgtgccg atcaaggccc cggtggccgg catcgcgatg ggcttggtgt
     4141 acgccgaggg caagtacacc accctcaccg acatcctcgg cgccgaggat gcctttggtg
     4201 acatggactt caaggtcgcc ggcaccgccg acgcgatcac ggccctccag ctcgacacca
     4261 agatcgacgg catccccgcc gacgtgctgg tggccgcgct ggcccaggcc aaggaagcac
     4321 gcctgcagat cctcgacaac atggccgcct gcatcgccgc gccgcgtgac gaggtcaacg
     4381 agaccgcgcc gaagatcgtg tcgttcaacg tgccgctcga caaggtcggc gagatcatcg
     4441 ggcccaaggg caaggtcatc aacacgatcc agcaggagac cggcgccgac atcagcgtcg
     4501 acgacgacgg cgtgcatggc atnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4681 nnnnnnnnnn nnnnnnncca agctcggcaa tggcaagcgg atcaacgccg tcgaagacgt
     4741 gttgaccctc ggcgacgaga tcgaggtgcg ggtcgacgag atcgacgaca agggcaaggt
     4801 cagcctcagc cccacgtcgt tcaccgcgcc tgccggtggc agtgacggtg gcgaggcccc
     4861 gcgtgagcgt ggtcctcgtc gtgagcggca gtccgactcg tcgg
//
LOCUS       DAORNF010000164         4881 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_12909_length_4881_cov_1.987360, whole genome shotgun sequence.
ACCESSION   DAORNF010000164 DAORNF010000000
VERSION     DAORNF010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4881
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12909_length_4881_cov_1.987360"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>312
                     /locus_tag="PLV68_07825"
     CDS             <1..>312
                     /locus_tag="PLV68_07825"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HRE01195.1"
                     /translation="EDIAVLDLVSQGRIDLLVAAGYVPAEFAMFGKAMNERGKLMEEG
                     VTALRKAWTGEPFEFRGETVLVRPRPFQQPGPPIIMAGDSRAAARRAARIGDAYAPMA
                     G"
     assembly_gap    313..393
                     /estimated_length=81
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <394..704
                     /locus_tag="PLV68_07830"
     CDS             <394..704
                     /locus_tag="PLV68_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01196.1"
                     /translation="YVSEDPDASWATLAPHLLHVSNSYAKWMEDDQRTGSFVHADSVD
                     ELRAGEVFEIYTPDECAAKLREWGSITIDPLFGGIAPDVAWECLELIRDKVLPQLDDA
                     "
     gene            742..1551
                     /locus_tag="PLV68_07835"
     CDS             742..1551
                     /locus_tag="PLV68_07835"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRE01197.1"
                     /translation="MSAETPLDGKVAVVTGASRGIGHAAAVRLAELGCDVVVAARTVE
                     PRPDLAGSITQTAEQVRALGRRCLPVAADMTSDADIANLAAATLAEFGRVDILINNAA
                     ALDERMYESFWDMTPESWRYQVELNLTSPWMTLKAFAPAMREQGGGLVVNMTSALASA
                     PLNPNLPGHGSTGAAYTTTKSALSRLTSDLSKELVPHGISIVALHPGFVKTDNGERLA
                     PVGGFDMSKASGSTDAPMAALSHLVTNDPVQWSGTVVFAPQFANDLGLLDG"
     gene            1566..>2020
                     /locus_tag="PLV68_07840"
     CDS             1566..>2020
                     /locus_tag="PLV68_07840"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRE01198.1"
                     /translation="MPITPTHHDLVTLLLGGAVHGDAPCFGFIPDGAPEHSEQIGFAA
                     FVDRARRVARGLAELGVVRGSRVALFARPDVDFMTAMAATLIAGAVVVPINHQFKHRE
                     LAGYLGIVDPVLIVTDRDTDALSVAVRPDLTRVIGGASRVGEARAGGEVS"
     assembly_gap    2021..2030
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2031..3204
                     /locus_tag="PLV68_07845"
     CDS             <2031..3204
                     /locus_tag="PLV68_07845"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fatty acid--CoA ligase family protein"
                     /protein_id="HRE01199.1"
                     /translation="MTITGLAGCELAEPVTVEPDDAAFIMHTSGTTGLPKGVVRSHGT
                     YARFATLWADRYMTAGDAVLDFMPMYHQGGVMMSFLPAFLRGLPVWQLERLSVASFWA
                     AAERHATPWAILMPPVPSYFVLQAEAAGEAPTTTPLAWAMTGGRVDHWAAMQERFGIV
                     GHSGYGSTETTMVTMTGDRADAPASPAVLHGPLGGFACGRPIEGWNEVRVVLDDRRLA
                     GPGQPGQLEFRGPGVFQEYFANPAATAAAFTADGWFHPGDVGYLDGDGQLFMIDRDRD
                     LIRRSGENISPREIEDVLHDHPAVEEAAVVGADDALRGQEIRACVVLVAGADVTAGEL
                     FEHCEAHLSSFKVPRYLDMRAELPHTPTMKVRKEMLVAETDAARWIDRSASQRNVV"
     assembly_gap    3233..3242
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3259..4881
                     /locus_tag="PLV68_07850"
     CDS             3259..4881
                     /locus_tag="PLV68_07850"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRE01200.1"
                     /translation="MRFPDFNGSQATTMANGRNITWLLQTRTERRPDHPFIVWEPLNG
                     PTETFTYTTFSQRVAQVAAGLRQRDVRQGERVVVHLENCPEFLFAWFALARIGAVAVC
                     TNARSSVDEIRHFGEHSQAVAAITQPKFAGQVAQALPGLRWIAVTETDAGEAVATGDR
                     PGASDAFASLYGDAGGGTPADVGAFDPAWIQYTSGTTSRPKAVVLTHANALWGGKVSA
                     LQEDLRSTDVHLVHLPLFHINALCYSVLSSLFVGATAVLQPRFSASRFWDVSLRHGCT
                     WASMIPFAIRALAAREIPAEHSYRIWGAGASSPPDEALFRVRMLAWYGMTETVIHAVR
                     DNKFETGSHPVIDEVYSPGRPYAMGRPSPAYEVALLHEDGTRITPGETGSVFVRGVPG
                     LSLFAEYLHDQAATREAVDERGWLHTGDTATLHEDGYLSFADRAKDLLKVGGENVAAS
                     EIERVIVAVAGVSEVAVVAAKHRMLDEVPVAFVIGSGANPDLAADVLAACKAQLADFK
                     VPREVRVVDEMPRSTLNKIAKAELRKTLPTFG"
BASE COUNT          755 a         1608 c         1623 g          794 t
ORIGIN      
        1 ccgaagacat cgccgtgctc gacttggtga gccaagggcg gatcgatctg ctcgtcgctg
       61 ctggctacgt gccagcggag ttcgccatgt tcggcaaggc gatgaacgag cggggcaagc
      121 tgatggagga gggggtcacc gcgctgcgca aggcatggac cggcgaaccg ttcgagttcc
      181 gcggcgagac cgtgctcgtg cggccgcgac cgttccaaca gccggggcca ccgatcatca
      241 tggccggtga ctcgcgtgcc gcggccaggc gtgcggcgcg catcggtgac gcgtatgcgc
      301 cgatggccgg agnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntctacgt gtccgaggat ccagacgctt
      421 cctgggccac gctcgccccg cacctgcttc acgtcagcaa ctcgtacgcg aagtggatgg
      481 aggacgacca gcgcacgggg tcgttcgtcc acgccgacag cgtcgacgaa ctgcgcgctg
      541 gagaggtgtt cgagatctac acgccggacg agtgcgcggc gaagttgcgc gagtggggga
      601 gcatcaccat cgacccgctg ttcgggggca tcgcccccga cgtcgcatgg gagtgcctcg
      661 agctcatccg tgacaaggtg ttgccacagc tcgacgacgc ctgatcgatc acaagacaaa
      721 acgaaccgaa agcgagcaac gatgagtgct gaaacaccct tggatggcaa ggtcgccgtc
      781 gtcaccggcg cgagccgcgg catcggtcac gctgcggcag tgcgcctggc cgagctcggc
      841 tgcgacgtgg tcgtcgcagc tcgaacggtc gagcctcgcc cagatcttgc cgggtcgatc
      901 acccagactg ccgagcaggt ccgtgccttg ggccgccggt gtctgcccgt cgccgccgac
      961 atgaccagcg acgcggacat cgccaacctg gccgcggcca cgctcgccga gttcgggcgt
     1021 gtcgacatct tgatcaacaa cgccgccgcc ctcgacgagc ggatgtacga gagcttctgg
     1081 gacatgaccc ccgagagttg gcgctatcag gtcgagctca acctgacgtc tccatggatg
     1141 acgctgaagg ccttcgcgcc ggcgatgcgc gagcagggtg gcgggctggt cgtcaacatg
     1201 acctcggcgt tggccagcgc accgctcaac cccaacctgc ccggccacgg gagcaccggc
     1261 gcggcctaca cgaccaccaa gtcggccctg agccggttga cgtcagacct cagcaaggag
     1321 ctcgtcccgc acggcatctc gatcgtcgcg ctgcacccgg gcttcgtcaa gacggacaac
     1381 ggtgagcggc tggcgccggt cggcggcttc gacatgtcga aggcgtcggg gagcaccgac
     1441 gcgccgatgg cggcgctctc tcatctggtc accaacgacc cggtccagtg gtcggggacc
     1501 gtcgtctttg cgcctcagtt cgccaacgac ctcggcctgc tcgacggctg acggccgacc
     1561 ggtccgtgcc catcaccccg acccatcacg acctcgtcac gctgctgctg ggcggcgcgg
     1621 tgcacggcga tgctccgtgc ttcggcttca tccccgacgg tgcccctgag cacagcgagc
     1681 agatcggctt tgccgccttc gtcgatcgcg cccgccgagt tgcgagaggc cttgcggagc
     1741 tcggagttgt ccgggggtcg cgtgttgcgc tgtttgcccg gcccgacgtc gacttcatga
     1801 ccgcgatggc ggccacgttg atcgctggag ccgtggtcgt tccgatcaac catcagttca
     1861 agcaccggga gctggccggc tacctcggca tcgtcgaccc ggtgctgatc gtgaccgaca
     1921 gggacaccga tgcgctgtcc gtcgccgtgc ggccagacct gactcgggtg atcggcggtg
     1981 cttcgcgcgt cggcgaggcg cgcgccgggg gtgaggtttc nnnnnnnnnn gatgacgatc
     2041 accgggttag ccgggtgcga actggcagaa cccgtgaccg tcgagccgga cgacgcagcg
     2101 ttcatcatgc acacctcggg gacgaccgga ttgcccaaag gggtcgtgcg cagccacgga
     2161 acgtacgctc gctttgcgac gttgtgggcc gatcgctaca tgaccgccgg tgacgccgtc
     2221 ctcgacttca tgccgatgta ccaccagggc ggcgtgatga tgtcgttcct gcccgccttc
     2281 ctccgcgggt tgcccgtctg gcaactcgaa cggctgtcgg tcgcgtcgtt ctgggccgcg
     2341 gccgaacgcc acgcgacacc ctgggcgatc ctgatgcccc cggtgccgag ctacttcgtt
     2401 ctccaggccg aggcagccgg cgaggcaccg acgacgacac ctctggcgtg ggccatgacc
     2461 ggtgggcggg tcgaccactg ggccgcgatg caggagcggt tcgggatcgt cggtcacagc
     2521 ggatacggct cgacggagac gacgatggtg acgatgaccg gggatcgcgc cgacgcgccg
     2581 gcgtcacctg ctgtgttgca cggcccgctg ggtggttttg cctgcggccg tccgatcgag
     2641 ggctggaacg aggtgcgcgt cgtgctcgac gaccggcggt tggctggccc cggtcagccc
     2701 ggccagctcg agttccgtgg acccggggtg ttccaggagt acttcgccaa cccggcggcg
     2761 accgcggcgg cgttcaccgc tgacggctgg tttcaccccg gggacgtggg ctacctcgac
     2821 ggtgacggcc agctgttcat gatcgatcgc gaccgcgatc tcattcgtcg ctccggcgag
     2881 aacatctcgc cccgagagat cgaggacgtc ctccacgatc acccggcggt cgaagaggct
     2941 gccgtggtcg gcgccgacga cgcgctgcgt ggtcaggaga tccgcgcgtg cgtggtgctc
     3001 gtcgccggtg cagatgtcac tgccggtgag ctgttcgagc actgcgaggc acatctcagc
     3061 agcttcaagg tgccgcgcta cctcgacatg cgcgcggagc tgccccacac gccgaccatg
     3121 aaggtgcgca aggagatgtt ggtcgccgag accgacgcgg caaggtggat cgatcgaagc
     3181 gcttctcagc gaaacgtcgt ctgatgaagg cgtcgacaaa caaacgatcg gtnnnnnnnn
     3241 nnactagctt gcagagccgt gagatttcct gacttcaatg gttcgcaggc caccacgatg
     3301 gccaacgggc gcaacatcac atggctgttg cagactcgca cagagcggcg ccccgatcat
     3361 ccgttcatcg tgtgggagcc gctgaacggt ccgacggaga cgtttaccta caccacgttc
     3421 tcgcagcgcg tcgcccaggt cgcggccggg ctgcgccagc gcgatgtgcg tcagggtgag
     3481 cgcgtcgtcg tccacctgga gaactgtccc gagttcctgt tcgcgtggtt cgcactggcc
     3541 cgcatcggtg ccgtcgccgt gtgcaccaac gcccgctctt cggtcgacga gatccgccac
     3601 ttcggggagc actcccaggc ggtggctgcc atcacccagc cgaagtttgc cggccaggtc
     3661 gcgcaggctc tgccggggct gcgctggatc gccgtcaccg aaaccgacgc gggcgaagcc
     3721 gtcgctaccg gtgatcgccc aggtgccagt gacgcgttcg ccagcctgta cggcgacgcc
     3781 ggcggcggta cgccggccga tgtcggcgcg ttcgatccgg cctggattca gtacacgtcc
     3841 gggaccacct cgcgacccaa ggccgtggtg ctgactcacg ccaacgcgct ctggggtggc
     3901 aaggtcagcg cactccaaga agacctgcgc agcaccgacg tgcatctcgt gcacctcccg
     3961 ctgtttcaca tcaacgcgct gtgctactcg gtgctgtcgt cgctgttcgt gggcgccaca
     4021 gcggtgttgc agccgcgctt ttcggcgtcg cgcttctggg acgtcagcct ccggcacgga
     4081 tgcacgtggg cgagcatgat cccgttcgcc atccgtgcct tggcggcacg cgagatcccc
     4141 gccgaacaca gctatcggat ctggggcgcc ggtgcgtcgt cgccgcctga cgaggcgctg
     4201 ttccgcgtcc ggatgctggc ctggtacggc atgaccgaga cggtcatcca cgccgtacgc
     4261 gacaacaagt tcgagacggg ctcccacccg gtgatcgacg aggtctacag cccgggccgg
     4321 ccatatgcga tgggccgtcc gtcgccggcc tacgaggttg cgctgctcca cgaggacggc
     4381 actcggatca cccccgggga gaccgggtcg gtgttcgttc ggggggtccc tggcctgtcg
     4441 ctgttcgccg agtatctcca cgatcaggct gcgacgcgcg aggcggtcga cgagcgtggc
     4501 tggctgcaca ccggcgatac ggcgacgctg cacgaggacg gctacctgag cttcgccgac
     4561 cgggccaagg acctgctcaa ggtcggcggc gaaaacgtcg cagcgtcgga gatcgagcga
     4621 gtcatcgtcg ccgtcgcggg cgtcagcgag gtcgccgtcg tcgccgcgaa gcaccggatg
     4681 ctcgacgagg ttccggtcgc gttcgtgatc ggttccggcg ccaacccgga cttggccgcc
     4741 gacgtcctcg cagcctgcaa ggcgcagctc gccgacttca aggttccgcg tgaggtccgc
     4801 gtggtcgacg agatgccgcg ttcgaccttg aacaagatcg ccaaagccga gctccgcaaa
     4861 accctcccaa ccttcgggtg a
//
LOCUS       DAORNF010000165         4871 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_12945_length_4871_cov_1.922550, whole genome shotgun sequence.
ACCESSION   DAORNF010000165 DAORNF010000000
VERSION     DAORNF010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4871
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12945_length_4871_cov_1.922550"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="PLV68_07855"
     CDS             complement(<1..439)
                     /locus_tag="PLV68_07855"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRE01201.1"
                     /translation="MNSGDEIIRTEGLTKVYPGGITAVDQLDLVVYQGEIFGLLGPNG
                     AGKSTTAGMLTTRVIPTSGSAFVGGIDVVRRPTQAKQVIGVVPQSNTLDRSLTVAENL
                     YFHGRFFGMPAKTAKAESAALIDRIKLNRFANHSVAALSGGMAQ"
     gene            complement(436..894)
                     /locus_tag="PLV68_07860"
     CDS             complement(436..894)
                     /locus_tag="PLV68_07860"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRE01202.1"
                     /translation="MTQSRENIGRKLALTARTVRAYGDQQMADAGSSMTVAIIAKILA
                     AEPGLSQRELADRMGVEGPTMVRHLDRLEADDIVVRTRDTQDRRVVRITLTDTGHRLH
                     RRLQKVSVRSQQQLTTIFTPDELDRFEQYLDRIKAHATDLVNTGEKAVAS"
     gene            complement(1018..1701)
                     /locus_tag="PLV68_07865"
     CDS             complement(1018..1701)
                     /locus_tag="PLV68_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRE01203.1"
                     /translation="MAGKQQDRVRVVIVDDDALVRSALRLMLGGLSDIEVVGEAADGR
                     QGVALADEVQPDVVLMDVRMPVMDGVTATAALCATARPPRIIVLTTFDADDYVLGAIA
                     AGADGFLLKDTEPAEIVDAIRRVFAGEPMLSPKATETLIRRVREAAEPDASPDDTGRA
                     ARARLASLTERERDVAEPIGRGLSNAEIATELFLSVPTVKAHVSRVFDKLGVTNRVHI
                     AILVHHATR"
     gene            complement(1740..2924)
                     /locus_tag="PLV68_07870"
     CDS             complement(1740..2924)
                     /locus_tag="PLV68_07870"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRE01204.1"
                     /translation="MTAAPAFSPPPVSAWGHVWRVGIVVFISAWAVAEDYARWWDGTR
                     WRIALDVVVGAVALAVVWRYRRRMPFLVAVAVLGAPVAGGAALGPVVWSLVSATTTRR
                     YWQVGALAATVLGIGTVATFFATLDEDEPRWAILTGLALMISAIFGWGLYIGSRRELL
                     GTLHQRAERAEAERELRARQTRTDERARIAREMHDVLAHRISQISMHTGALVFREDLS
                     ADALRAGIADVHERANLALDDLRSVLGVLRDPDTGRAMDRPQPTYGDIAGLVAEARAA
                     GARVDYVDRLGDEASEAKVPTALGRTLYRVVQEGITNANKHAPGARLMVGVSADDAGN
                     DERGVRVVMSNPVGFGPSVTPGAGLGLIGLRERVELAGGQLSQGREGDLFVVRAVLPW
                     PP"
     gene            3116..>3269
                     /locus_tag="PLV68_07875"
     CDS             3116..>3269
                     /locus_tag="PLV68_07875"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRE01205.1"
                     /translation="MIDIQRLTKTYGDFTAVDDISLIARAGRVTGFLGPNGAGKTTTM
                     RILAGLA"
     assembly_gap    3270..3435
                     /estimated_length=166
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3436..3681
                     /locus_tag="PLV68_07880"
     CDS             <3436..3681
                     /locus_tag="PLV68_07880"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRE01206.1"
                     /translation="RVDERLERVALTQAEARRTVRNYSLGMRQRLGVAHALIGDPHVL
                     ILDEPANGLDPGGIRAMRTLLRDYADRGGTVLLSSPA"
     gene            4089..4613
                     /locus_tag="PLV68_07885"
     CDS             4089..4613
                     /locus_tag="PLV68_07885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01207.1"
                     /translation="MTAAIASPRPTVGFAPADTAPIPLERLVGVELRKLVDTRSGRWL
                     LIAQAIAIVVAMAVRTIIAGTEGESISLWDYAWLAGSVMAFLLPVMGIMAITTEWSQR
                     TAMTTFTLEPRRARVVAAKLAAVVVTSLVAVSAAIVIGIAASAVAASVGIDTEVMADT
                     AKEKAGGTSTPPKQ"
BASE COUNT          771 a         1663 c         1502 g          769 t
ORIGIN      
        1 gttgcgccat gccgcccgac agcgccgcca ccgaatggtt ggcgaagcgg ttgagcttga
       61 tgcgatcgat cagagcggcg ctttcggcct tggccgtctt ggctggcatc ccgaagaagc
      121 gaccgtggaa gtacaggttc tctgccacgg tcaggctgcg atcgagcgtg ttcgactgcg
      181 gcaccacgcc gatcacctgc ttggcctggg tcggtcgccg tacgacgtcg atgccgccga
      241 cgaatgcgct gccgctggtg ggaatgacgc gggtggttag cattccagca gtggtcgatt
      301 taccggcccc gttcggtccg agcagcccga agatctcgcc ctggtacacg acgagatcga
      361 gttggtccac cgccgtgatg ccacccggat agaccttcgt caggccctcg gtgcggatga
      421 tctcgtcgcc ggagttcacg acgccaccgc cttctcaccg gtgttgacca gatcggtggc
      481 gtgggccttg atgcggtcga ggtactgctc gaagcggtcg agctcgtcgg gggtgaagat
      541 ggtcgtgagc tgctgctggc tgcggaccga gaccttctgc aggcgccggt gcagacgatg
      601 gccagtatcg gtcagggtga tccgcaccac ccgcctgtcc tgcgtgtcgc gggtgcggac
      661 aacgatgtcg tcggcctcca gacggtcgag gtggcgaacc atcgtcgggc cttccacacc
      721 catccggtcg gcgagctcgc gctgcgacag gccgggctcg gcagcgagga tcttggcgat
      781 gatcgcaacc gtcatgctcg atccggcgtc ggccatctgt tggtcgccgt atgcccgcac
      841 cgtccgggct gtgagcgcca gcttgcgccc gatgttctcg cgggattgcg tcatgaggta
      901 atcataagtg atcgaatgat tcgctggcaa ctgattgtgc ggcaatccga gcgaccggat
      961 cgcggcactt cccgtctggt cgctctgggc agactgaacc cgaaccgtcg ggtgaggtca
     1021 gcgggtggcg tgatggacga ggatggcgat gtggacacgg ttggtcacgc cgagcttgtc
     1081 gaagacgcgt gacacgtggg ccttgacggt cggcaccgac aggaacagct cggtcgcgat
     1141 ctcggcattc gacaatccgc gtccgatggg ctcggccacg tctcgctcgc gctcggtcag
     1201 cgacgccaac cgggcccgcg cggcgcgccc ggtgtcgtcg gggctggcgt ctggctcggc
     1261 ggcctcacgc acccggcgaa tcaacgtttc ggtggccttt ggcgagagca tcggctctcc
     1321 tgcgaagacc ctgcggatcg catcgacgat ctcggcaggc tcggtgtcct tcagcaggaa
     1381 cccgtcagct ccagcggcga tcgctccgag cacgtagtcg tcggcatcga acgtcgtcag
     1441 cacgatgatc cgcggcggcc gagccgtggc gcacaacgct gcggtcgcgg tgacgccgtc
     1501 catcaccggc atccgaacgt ccatcaacac aacatcgggc tgcacctcgt cggccagcgc
     1561 cacaccctgg cgaccgtcgg ctgcttcgcc gacgacttcg atgtcggaca gtccaccaag
     1621 catcagccgc aacgccgaac gtaccaacgc gtcgtcgtcg acgatcacca cccgtactcg
     1681 gtcctgctgc tttccggcca tccctccgac actgcctggg tgggatcgat cgtcgcccgt
     1741 tacggcggcc acggcagcac cgcccgcacg acgaacaggt cgccctcgcg cccttgcgag
     1801 agttggccac cggccaactc gacccgctca cgcaacccga tcaaacccaa gccggcgccc
     1861 ggcgtgacgg acggcccgaa tccgactgga ttcgacatca ccaccctgac cccgcgctca
     1921 tcgttgcctg cgtcgtcggc ggagaccccg accatcagcc gtgccccagg ggcgtgcttg
     1981 ttggcattgg tgatgccttc ctgcacgact cggtacaacg tgcgccctag cgcagtcgga
     2041 accttcgcct cgctcgcttc gtctcccagc ctgtcgacgt agtcgacccg cgctccggcg
     2101 gcccgtgctt cggcgacgag accggcgatg tcgccgtagg tcggttgcgg tcgatccatg
     2161 gcacggcctg tgtccggatc gcgcagcacc ccgagcacgc tgcggaggtc gtccagggcc
     2221 aggttggcgc gctcgtgcac gtcggcaatc ccggcccgca acgcgtccgc gctgaggtct
     2281 tctcggaaca ccaatgcccc cgtgtgcatc gagatctgac tgatccggtg cgccaacacg
     2341 tcgtgcatct cgcgggcgat gcgcgcccgc tcgtcggtgc gtgtttgccg tgcccgcaac
     2401 tcgcgttcgg cctcggcgcg ctcggctcgc tgatgcagcg tgccgagcag ctcgcgtcgc
     2461 gacccgatgt acaaacccca cccgaagatc gccgagatca tcagcgccaa acccgtcagg
     2521 atcgcccacc gcggctcgtc ctcgtccaac gtcgcgaaga acgtcgccac cgtcccgatc
     2581 ccgagcacgg tcgcagcgag cgcgccaacc tgccagtacc gccgcgtcgt cgttgccgag
     2641 accagcgacc acaccacggg gccgagcgct gcgccgccgg cgactggcgc gcccaacacc
     2701 gcaacggcaa ccaggaacgg catgcggcgg cggtatcgcc acacgaccgc cagtgccacc
     2761 gccccgacca cgacgtccag agcgatccgc cagcgtgtcc cgtcccacca ccgggcgtag
     2821 tcctcggcga ctgcccacgc cgagatgaac acgacgatcc cgacgcgcca gacgtgcccc
     2881 catgccgaga ctggtggggg actgaacgct ggtgcggccg tcatcaccgc gtcagcgtag
     2941 acacagcgac cggccgggcg agtcggctga aggtcgcagc gtgaccccta ccttcgtcgc
     3001 tgtgcggggg atcgatggca gcgactctcg tcggggtcgt cagacgaccg agggcaccaa
     3061 acaagcgacc gttgcccgat gtgccgagcc gccgcacacc gtcaccatcg gagttgtgat
     3121 tgacattcag cgactcacca agacatacgg cgacttcacc gccgtcgacg acatctccct
     3181 catcgcccgc gcgggccggg tcaccgggtt tctcgggccg aacggcgcgg gcaagaccac
     3241 gacgatgcgc atcctcgccg gactggctcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnncgtgt cgacgagagg ctcgagcgcg tcgctctgac ccaggccgag
     3481 gcgcgtcgca ccgtgcgcaa ttacagcctc ggcatgcgtc agcgcctcgg cgtcgcgcac
     3541 gccctgatcg gcgaccccca cgtgctcatc ctcgacgagc cagccaacgg cctcgatccc
     3601 ggagggattc gcgcgatgcg caccctgctg cgcgactacg ccgaccgcgg aggcaccgtg
     3661 ctgctctcgt cgcctgcatg aaggcgagca gatcgccgac gagatcgtca tgatcggtcg
     3721 cggtcgcatt gtcgccgagg gcacaaccgc cgacctgctg accggtgccg gtaccagcgt
     3781 gcactcgacc gacgaccagg ctctcgctgc tgctgcccgt gtcgccgggc acatggtgat
     3841 catgcacgac tcggtcgctc ccgggatgac catcgacgcc gaacccgtcg aggtgggacg
     3901 cctcgcccgc gccgccggga tcgccctggt cgacctgcgc ccgtcgagca ccggtggtct
     3961 cgaagatctg ttcctggagc tcaccgccga cacccagcgc gagcaagccg accccatcga
     4021 acgcgatctc ggtgcgagcg gtgcgcccgg accgcaccag cccatgccac gacagcggag
     4081 gtctcagcgt gaccgccgca attgcatccc cgaggcccac ggttggcttc gcccccgccg
     4141 acaccgcacc gatcccgctc gagcgtctcg tgggcgtcga actgcgcaaa ctggtcgaca
     4201 cccgctcggg ccgctggctg ctcattgccc aggcgatcgc catcgtcgtt gcgatggcag
     4261 tacgcacgat catcgccggt accgaaggcg agagcatctc gctgtgggac tacgcctggc
     4321 ttgctggatc ggtgatggcg ttcctgctcc cggtcatggg gatcatggcg atcaccaccg
     4381 aatggtcgca gcgcacggcg atgaccacgt tcaccctcga accgcgacgc gcccgcgtcg
     4441 tcgctgccaa gttggctgcc gtggtggtca cctcgctcgt cgcggtcagc gcggcgatcg
     4501 tcatcggtat cgccgcgtcg gctgttgccg catcggtcgg catcgacacc gaggtgatgg
     4561 cggacaccgc caaggaaaag gccggtggca cgtcgacgcc tccgaagcag taagctctcg
     4621 caaagcttga tgaatgagcc ccggctgcgc aagcggccgg ggtttttcgt tgcgcgaaag
     4681 gtgcccttga ccttgcgcgg ggcatcgcct aaagcagccc atccgcaaag ccccggattc
     4741 gtccggggct ctgttcttgt tcgcaggaac cggatgcctg ggaccttcgg ggccctcata
     4801 cgaaccgaag ggtcgcaaga ccctcaaagc aacggaagac agaaagcatg gcactcaagt
     4861 cgtataaacc g
//
LOCUS       DAORNF010000166         4869 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_12955_length_4869_cov_1.597840, whole genome shotgun sequence.
ACCESSION   DAORNF010000166 DAORNF010000000
VERSION     DAORNF010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4869
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12955_length_4869_cov_1.597840"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="PLV68_07890"
     CDS             complement(<1..397)
                     /locus_tag="PLV68_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HRE01208.1"
                     /translation="MNDKLEDLRTRREQAFHAGSARAVERQHEKGKLLARERIEYFLD
                     PGSFQELDLLARHRAHAAGLEERPYTDGVITGWGTVDGRKVFVFSQDFTVFGGALGEV
                     FAEKIHKLMDLALSVGVTLIGLNDGAGARI"
     gene            474..1550
                     /locus_tag="PLV68_07895"
     CDS             474..1550
                     /locus_tag="PLV68_07895"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HRE01209.1"
                     /translation="MSLPLSPPIAPMLSKAAERIPTGDGWHYEPKWDGFRCVVFRDGD
                     DVVLTSRNERPFNRYFPELVDPLRAVLPERCVVDGEIVLPDRNGNGLDFDGLLQRIHP
                     AERRVRRLAAETPASFVAFDMLVDGDKLLLDDPLAFRLAHLRVALTDAEPPIYLTPSS
                     TDPAVAEQWFHRFEGAGLDGVVAKRLTDVYTPDKRTLVKVKHSRDADCVVAGYRVHKD
                     GKGVGSLLLGLYDDEGRLHHVGVTAAFTAKFRAELLTVLEPLTHDALAGHPWADWADP
                     SAHAEARLPGAQSRWNAKKDLSWVPLRVEWVAEISFGQLQNGRFRHGVQFHRWRPDRM
                     PQSCTYDQLDVADPVSFDELMANA"
     gene            complement(1653..>3353)
                     /locus_tag="PLV68_07900"
     CDS             complement(1653..>3353)
                     /locus_tag="PLV68_07900"
                     /inference="COORDINATES: protein motif:HMM:NF017262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HRE01210.1"
                     /translation="SQPTAGATLPTPPINIVITFDQTIGPSVSARIVCNGNPQPAAPT
                     NPSQDLKSIVVDLAAVVLPKGTCDVHWAVIPTTETVVSSGTITFQIANDASAPTSSTV
                     AGSAATPTTTASSGTSADAETTTGDLSGPLGLARLLSMTTLAMLFGAIVLIAIAWPEG
                     TDYVATIRYLRIVWLAAVISTGVMVACMVAQVSGSSFTSALMPTAWTDLTDTTPGLAA
                     LARMGLTVAVGWVVIQPDRINVQQLPSLALAAFAVATMGFTRSGGDLELIGYAAGIFH
                     ALAMAVWFGGVVLLARVVLLGPGEEDLVHAVRGFSKMATPAIAITVLTGAFQLYRLDR
                     GHLTDSNHGRLLVVKILPVIGMVIVGTATRQFIRARFAREDVMTTANASRLRRVVGME
                     AMLGLAVLGLTAWMLNTQPANLRVGVRTAADYRVHIPLATADGVLDVAFGLDPGRVGT
                     NEMLLEVVAPSSGITSITINFTPPAAQPNATAVSIAITELTGAGAIHIPRSVGIPLDQ
                     PGEWNVGVDIALANGTVLRQTVLVTITGTGTIVTEIPVVTSPPVTAVATTTTTTPPAG
                     "
     assembly_gap    3354..3467
                     /estimated_length=114
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3747..3932
                     /estimated_length=186
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3933..>4461)
                     /locus_tag="PLV68_07905"
     CDS             complement(<3933..>4461)
                     /locus_tag="PLV68_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01211.1"
                     /translation="ASEVLSLRKLLADAASSLDDIEAPADLSRGARSFVNALKSQSDV
                     AGQLADTMSSGGDITTAVGDLYTAAGTTDEAARAIGALECVYLTPNLAALAPDLAPPT
                     STTEVPPTTDGTDTTDTTDTTDGTDTTDGTDTTTPEPVYPENLNEIANAPAGFSWEPN
                     FNQINVADLYTGTGEF"
     assembly_gap    4462..4521
                     /estimated_length=60
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4650..>4869)
                     /locus_tag="PLV68_07910"
     CDS             complement(4650..>4869)
                     /locus_tag="PLV68_07910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01212.1"
                     /translation="QIDAIWTAEAVASGTEITTPGGLLARINIGVGDTGQFDAAAAVV
                     PNNSIAIFTTTGGQDLASLIDAIYDANV"
BASE COUNT          704 a         1451 c         1614 g          740 t
ORIGIN      
        1 ggatgcgggc gccggcgccg tcgttgaggc cgatcagggt gacgcccacc gagagggcga
       61 gatccatcag cttgtggatc ttctcggcga agacctcgcc gagtgcgccg ccgaacacgg
      121 tgaagtcctg gctgaacacg aataccttgc ggccatcgac ggtgccccag ccggtgatca
      181 caccgtcggt gtaggggcgc tcttcgagcc cggccgcgtg ggcgcggtga cgagcgagca
      241 ggtcgagctc ctgaaagctg ccgggatcga ggaaatactc gatgcgctca cgggccagca
      301 acttgccctt ctcgtgctgg cgctcgacgg cccgggccga gcctgcgtgg aacgcctgtt
      361 cgcgccgggt gcgcaggtcc tcaagcttgt cgttcatcgg gttggtcacg gccacgaagc
      421 tagtgccgcg ccgggctctt gtggaactta cgacgatcca ccacgctgtg gtcatgtcgc
      481 tgccgttgag cccgccgatc gccccgatgc tgtccaaggc cgcggagcgc attcccaccg
      541 gtgacgggtg gcactacgag ccgaagtggg acgggtttcg atgcgtcgtg ttccgtgacg
      601 gagacgacgt cgtcctgacc agccgcaacg agcggccgtt caaccgctac ttccccgagt
      661 tggtcgatcc gctgcgggct gtgttgcccg aacggtgcgt ggtcgacggt gagatcgtgt
      721 tgcccgaccg caacggcaac gggctcgact tcgacggcct gctccagcgc atccatccgg
      781 ccgagcgccg agtccgacgc ctggccgcag agacacccgc cagcttcgtc gccttcgaca
      841 tgctggtcga cggcgacaag ctgctgctcg acgacccact ggccttccgg ctggcccacc
      901 tgcgcgttgc gctgaccgac gccgagccac cgatctacct gacgccgtcg tccaccgacc
      961 ccgccgtggc cgagcagtgg ttccatcgct tcgagggtgc gggtctcgac ggggtggtcg
     1021 ccaagcggct gaccgacgtg tacacccccg acaagcgcac gttggtcaag gtcaagcact
     1081 cccgcgacgc cgactgcgtg gtcgccgggt accgcgtcca caaggacggc aagggggtcg
     1141 gctcgctgct gctgggcctg tacgacgacg agggccggtt gcaccacgtc ggcgtcacag
     1201 cggccttcac cgccaagttc cgggccgagc tgttgacggt gctggagccg ctgacccacg
     1261 acgcgctggc ggggcatccg tgggccgact gggccgaccc gagcgcccac gccgaggccc
     1321 gtctgccggg cgcgcagagc cgctggaacg ccaagaagga tctcagctgg gtgccgctgc
     1381 gcgtcgaatg ggtcgccgag atcagcttcg gccagttgca gaacggacgg ttccgccacg
     1441 gtgtgcagtt tcatcggtgg cgtcccgacc gcatgccgca gagttgcacg tacgaccagc
     1501 tcgacgtcgc cgatcccgtc tccttcgacg agctgatggc caacgcatga ggtgccgcgg
     1561 cgcttgatgc cggccaaccg gcgatggggc caaccgctga cgaggccaac cgctcacgag
     1621 gccaacgggt gacgtcggcc tggctggcgg gatcagccgg ccggtggggt cgtggtggtc
     1681 gtggtcgcca ccgcggtgac cggcggactg gtgacgaccg gaatctcggt cacgatggtg
     1741 ccggtaccgg tgatggtgac caggacggtc tggcgcagca cggtgccgtt ggccagcgcg
     1801 atgtcgacgc cgacgttcca ctcgcccggc tgatcgagcg ggatgcccac cgagcggggg
     1861 atgtggatcg ccccggcgcc tgtcagttcg gtgatggcga tgctcaccgc ggtcgcgttc
     1921 ggctgtgccg cgggtggggt gaagttgatc gtgatgctgg tgatgccgct ggacggagcg
     1981 accacttcga gcagcatctc gttggtgccg acgcggcccg ggtcgagccc gaaggccacg
     2041 tcgagcacac cgtcggccgt ggccagcggg atgtggacgc ggtagtcggc tgcggtgcgt
     2101 acgccgacgc gcaggttcgc gggctgcgtg ttgagcatcc acgcggtcaa ccccagcacg
     2161 gccaagccga gcatcgcttc catgcccacc acccggcgca gccgactggc gttggcggtg
     2221 gtcatcacgt cttcgcgggc gaaccgggcg cggatgaact gacgggtggc ggtacccacg
     2281 atcaccatgc cgatcaccgg caggatcttg acgaccagca gccgcccgtg gttggagtcg
     2341 gtgaggtgcc cgcggtcgag ccggtagagc tggaacgcgc cggtcagcac cgtgatcgcg
     2401 atcgccgggg tggccatctt ggagaaaccg cggaccgcat ggacgagatc ctcttctccg
     2461 gggccgagca gcaccacgcg ggccaacagc acgaccccgc cgaaccacac cgccatcgcc
     2521 agcgcgtgga agatgccagc ggcgtagccg atcagctcca agtccccgcc cgaacgggtg
     2581 aagcccatgg ttgcaacggc gaaggccgcg agcgccagcg agggcagttg ttgcacgttg
     2641 atgcgatcgg gctggatgac gacccagccg accgcgacgg tcaaccccat gcgggccaga
     2701 gcagccaggc ccggggtcgt gtcggtcaga tcggtccatg ccgtcggcat cagggccgag
     2761 gtgaacgacg agcccgacac ctgggcgacc atgcacgcga ccatcacccc cgtgctgatc
     2821 acagcggcca gccagacgat acggaggtag cgaatggtcg ccacgtagtc ggtgccttct
     2881 ggccaggcga tggcgatcag cacgatcgcc ccgaacagca tcgccagcgt cgtcatcgac
     2941 agcagccggg ccaagccgag cgggcccgac aggtcgccgg tggtcgtctc cgcatctgcg
     3001 gaggtgccgc tggacgccgt ggtggtcggc gtcgcggccg aaccggcgac ggtcgacgac
     3061 gtgggagcac tggcatcgtt cgcgatctgg aatgtgatcg ttccggatga aacgaccgtc
     3121 tcggtggtgg ggatcaccgc ccagtggacg tcacaggttc ctttgggcaa gaccaccgcg
     3181 gccagatcga cgacgatgct cttcaggtct tgtgaggggt tggtgggtgc tgctggctga
     3241 gggttgccgt tgcagacgat gcgggcgctg accgacggcc cgatcgtctg atcgaaggtg
     3301 atcacgatgt tgatcggtgg cgtcggcagc gtggccccgg cggttggttg cgannnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntgc cctgctgctg
     3481 gaatgcgctg caccgagaca tgaaagttcc ccaaccgtag tcaaggcagc cgaaaacggc
     3541 ggtcaccggt gatcgcgcgc cgtcggcacg ccgatatggc agcgacggtc agccgcaatc
     3601 agaacgggtt ggcggcgagg tatgcgtcga gcagcggctt gccgtcgacc ccggccagcg
     3661 tgcacacggt gaagccactg ctgcccgagt aataggccca gcagtcggtc tcttcgaatg
     3721 cttccgcgcg gaacgcgacc gtgagcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncgaattcg ccggtgcccg tgtacagatc
     3961 ggcgacgttg atctggttga agttcggctc ccacgagaac ccggcgggtg cgttggcgat
     4021 ctcgttgagg ttctcggggt acaccggctc gggcgtcgtc gtatccgtgc cgtccgtggt
     4081 atcggtgccg tcggtggtat cggtggtatc ggtggtatcg gtgccgtcgg tggtcggtgg
     4141 tacctcggtg gtgctggtcg ggggcgcgag atcgggcgcc agcgcggcga ggttgggggt
     4201 gaggtagacg cactccaacg cgccgatggc ccgtgctgct tcatcagtgg tacccgccgc
     4261 ggtgtacaga tcaccgacgg ccgtggtgat gtcgccaccg ctgctcatcg tgtcggccag
     4321 ttgacccgcg acgtcggact gactcttcag cgcgttgacg aagctgcgcg caccgcggga
     4381 caggtctgca ggcgcctcga tgtcgtcaag gctgctggcc gcatcggcca gcagtttgcg
     4441 cagggagagc acctcggacg cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nggcctcggc ctggtcgagg tactcgtcgg tgggttgtga
     4561 caccgtggca tcgctgccgc acgccgtggc cacgctggcc aacaccagcc cggccatggc
     4621 cagtcgggcg acagcagcgt ggcggcgcat cagacgttgg cgtcgtagat ggcgtcgatc
     4681 agcgaagcca ggtcctggcc accggtggtg gtgaagatcg cgatgctgtt gttgggtacg
     4741 acggctgcgg cggcgtcgaa ctggcccgtg tcgccgaccc cgatgttgat ccgggcgagc
     4801 aaaccgccgg gcgtggtgat ctcggtgccc gaggcgacgg cttcggccgt ccagatcgcg
     4861 tcgatctgc
//
LOCUS       DAORNF010000167         4869 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_12956_length_4869_cov_1.388658, whole genome shotgun sequence.
ACCESSION   DAORNF010000167 DAORNF010000000
VERSION     DAORNF010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4869
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12956_length_4869_cov_1.388658"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    227..340
                     /estimated_length=114
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<341..824)
                     /locus_tag="PLV68_07915"
     CDS             complement(<341..824)
                     /locus_tag="PLV68_07915"
                     /inference="COORDINATES: protein motif:HMM:NF014669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015225 - biotin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015878 - biotin transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HRE01213.1"
                     /translation="MTTAMHPQAGHVPTRRLVLADLIPNMRLRSAALVVAGALLTAAA
                     SQVRIPLGFTPVPINLATFAAALTGGALGMRRGVASMTLYLLAGIVGLPFFAGGTSGW
                     SIVSGATGGYLVCYPVMAAIVGWAAQRGHDRRVGSFVAAVVAANAVLYLVGALWLGHV
                     V"
     gene            974..1540
                     /locus_tag="PLV68_07920"
     CDS             974..1540
                     /locus_tag="PLV68_07920"
                     /inference="COORDINATES: protein motif:HMM:NF014906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HRE01214.1"
                     /translation="MSEPLTRTDIVEAALGLLAGGGLHALAMRRIAAELGIQQSALYW
                     HFDNKQQLLSAVADQIVAPVHPVEQGSWSSRIIALSARLRRALLAYPDGAELVAAAIA
                     FRLGAQRPFQLFAGILADAGLGRDDAEIAASVLVHFTLGFTADEQQHHQAAALGAIMP
                     DPADPSSADDRFGRGVNLIVLGLTSQLA"
     gene            1631..>1963
                     /locus_tag="PLV68_07925"
     CDS             1631..>1963
                     /locus_tag="PLV68_07925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="group III truncated hemoglobin"
                     /protein_id="HRE01215.1"
                     /translation="MSAGTTDLWRGRDLDDPAEIAEMVRRFYADVAQDDLLGPMFNKV
                     ARVDWSEHLPKLTAFWCRALLSIPGYQGNPFRQHLLVHARQSFTIPQFVRWLDLFHDT
                     LDCGWAGPN"
     assembly_gap    1964..2138
                     /estimated_length=175
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2139..2370)
                     /locus_tag="PLV68_07930"
     CDS             complement(<2139..2370)
                     /locus_tag="PLV68_07930"
                     /inference="COORDINATES: protein motif:HMM:NF019998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HRE01216.1"
                     /translation="MGEHGAGEGATVLVRPEQIVADSQSAVRATVRSVQFNGPDVIVV
                     LVPDGVPVERDHGTGDDDTADEDSVQVRWPTAE"
     assembly_gap    2413..2581
                     /estimated_length=169
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2582..>3017)
                     /locus_tag="PLV68_07935"
     CDS             complement(<2582..>3017)
                     /locus_tag="PLV68_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRE01217.1"
                     /translation="LRIGGTPVATMGSGSSNVWVPPERRRVGIVPQHGALFPHLDVSG
                     NVRFGLHDRRRAPARARAAEMLDLVGLSGFERRKPHELSGGQQQRVALARALAPRPDV
                     VLLDEPFTALDAALRTRLQADVRAVLRESRATAVMVTHDPAEA"
     assembly_gap    3018..3027
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3189..>3857)
                     /locus_tag="PLV68_07940"
     CDS             complement(3189..>3857)
                     /locus_tag="PLV68_07940"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRE01218.1"
                     /translation="LSDVFAAARTTLLLGVLGAAATVVVALPVAMLSARHPGGLANGV
                     TTVAYAGHALPGIVVGLSLVFFGVRVATPLYQRMPMLIAAYVVVFLSLAIGALHTALA
                     QVAPELDDVARSSGRGPLRAWLAVTLPLAAPGAGVAAALVCIAVMKELPATLLLRPIG
                     TDTLATRLWAKTDAFSYAAAAPYAVALVVTASIPTALLTRFSMRQNKTGQDRRGQDRG
                     RGHG"
     assembly_gap    3858..3929
                     /estimated_length=72
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3930..>4513)
                     /locus_tag="PLV68_07945"
     CDS             complement(<3930..>4513)
                     /locus_tag="PLV68_07945"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRE01219.1"
                     /translation="RADLPLRAFWRVALAMPLAIPSYIAAWGWIGWSPSLAGFRGSVL
                     VLTLISYPYVYLPVLGALRRADPALAEVARSAGRSPAQVFVGVTLRQIRIAAFGGCVL
                     VALYTLSEFGAVSIMRYETLTKVIYTSYRASFDRTPAAVLGCVLVAITLVPLWLGVRS
                     SAAGRIARVGSGVGHDAATVRLGLWRWPAVAGCT"
     assembly_gap    4514..4630
                     /estimated_length=117
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          732 a         1519 c         1370 g          591 t
ORIGIN      
        1 ggccgagcac ctgcaacatc gtggtctcag cgtatcccca cgtcccgtcg tcgttgatgg
       61 tcaccggtcg gctcgaacga caccgtcgcc acgacccgta cgagcaaggg gttggcgcag
      121 atgccgaact cggtcggcca cactcactcg ggccgcacga ggagggccgc gtgcggttgg
      181 acctgctggt cagcggtcgc cgatcgcccg ccacgccgcg gggaacnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cgacgacatg tcccagccac
      361 agtgcgccga ccaggtagag caccgcgttg gcggcgacga cggcggccac gaacgagccg
      421 acgcgacgat cgtgaccgcg ctgtgcagcc cagcccacga tggccgccat caccggatag
      481 cagaccaggt agccgccggt ggcgcccgag acgatcgacc atccgctcgt cccgccggcg
      541 aagaacggca agccgacgat gccggccagg agatacagcg tcatcgaagc gacgccgcgg
      601 cgcatgccga gcgcgccgcc cgtgagcgcg gcagcaaagg tcgccaggtt gatcggtacc
      661 ggggtgaacc ccagcgggat gcgcacctgc gatgcagcag cggtgagcag tgccccggca
      721 accaccaggg cagcgctgcg caggcgcatg ttgggaatca ggtcggccag caccagccgg
      781 cgcgttggta cgtgacccgc ttgagggtgc atcgctgtgg tcatgggttc cgtccttcac
      841 gtcgtcgcca ctcgatggcg atcctgaaca ctgttcactg aacggcgttc atgatagcgc
      901 gaggctgccg cagcacacaa cggccgatcc ggcggagcac aatcgccact cctgcggagc
      961 acaatgaacg gtgatgagcg agcccttgac gcgaaccgac atcgtcgagg ctgcgttggg
     1021 cctgctcgcc gggggcgggc tgcacgcgct ggcgatgcgc cgtatcgcgg ccgagctcgg
     1081 cattcagcag agtgcgctgt actggcactt cgacaacaag cagcagttgc tgtcggctgt
     1141 cgccgaccag atcgttgcac cggtccaccc ggtcgagcag ggttcgtggt catcgcggat
     1201 catcgccctg tcggcgcgcc tgcgccgcgc cctgctcgcc taccccgacg gggccgagtt
     1261 ggtcgccgcc gcgatcgcgt tccgtctcgg cgcgcagcgg ccgttccagc tgtttgccgg
     1321 catcctcgcc gatgccgggc tcggacgcga cgacgcggag atcgccgcgt cggtgctcgt
     1381 tcacttcacg ctcggcttca ccgccgacga gcagcagcat caccaggcag ccgcactcgg
     1441 cgcgatcatg cccgacccgg ccgacccgtc aagcgccgac gaccgcttcg gacgcggcgt
     1501 caacctgatc gtcctcggcc tcaccagtca gctcgcctga tcgtgacgct caccggtagc
     1561 ggctcacccg gccgcggaac gggacctttg accccagacc gttcttctat acctcgctag
     1621 tgtttcccac atgagtgctg gcacgactga cttgtggcga gggcgcgatc tggacgatcc
     1681 ggccgagatc gcggagatgg tgcggcggtt ctacgccgat gtcgctcagg acgatctgct
     1741 cgggccgatg ttcaacaagg tggcccgcgt cgactggtca gaacacctcc ccaagctcac
     1801 cgcgttctgg tgtcgagcgt tgctgtcgat ccctggctat cagggcaacc cgttccgcca
     1861 acacctgctg gtgcacgcgc gtcagtcgtt caccatcccc cagttcgtgc gctggctcga
     1921 cctgttccac gacaccctcg attgtggctg ggccggcccc aacnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncc tcggccgttg gccaacgcac
     2161 ctgcaccgag tcctcatcag cggtgtcatc atcaccggtg ccgtggtcac gctccaccgg
     2221 cacaccgtcg ggtaccagca cgacgatcac atcgggtccg ttgaactgca ccgagcgcac
     2281 agtggcccgc accgccgact gcgagtcggc cacgatctgc tccggacgga ccagcacggt
     2341 ggctccttcg ccggcgccgt gttcgcccac gttgcgcacg gtgatcggac caaacggcgt
     2401 ccgcacctcc ccnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nggcctcggc cgggtcgtgc gtgaccatca cggcggtcgc ccggctctcc cgcaagaccg
     2641 cccggacgtc ggcctgcaag cgcgtccgca acgccgcgtc gagagcggtg aacggttcgt
     2701 ccagcaacac cacgtcgggg cggggtgcca gcgcgcgcgc cagcgcaact cgctgctgtt
     2761 gaccgccaga gagctcatgc ggcttgcgtc gctcgaaacc gctcagaccg accaggtcga
     2821 gcatctcggc cgcgcgggca cgagccggcg cgcggcgtcg gtcgtgcaag ccgaagcgga
     2881 cgttgccgct gacgtcgagg tgaggaaaga gtgcgccgtg ttggggcacg atgccgacgc
     2941 gccggcgctc gggcggcacc cacacgttgg acgatccgct gcccatcgtt gcgaccggcg
     3001 tgccgccgat gcgcagcnnn nnnnnnnggg ccgttcgaaa cccgcgatca accgcagcag
     3061 cgtggtcttg ccacagcccg atggtccgag gatcaccgcg atcgtgccgt cgtcgatgtc
     3121 gaggtcgacg tcgtcgacgg cctgcgtcgc gccgaaggcc ttcgacacgc cctggatctc
     3181 gatgcggctc atccgtgtcc tctccctctg tcctggccgc gtctgtcttg gccggttttg
     3241 ttctggcgca tgctgaatcg tgtcaggagc gcggtcggga tcgacgccgt caccacgagc
     3301 gccaccgcgt acggtgccgc cgccgcatag ctgaacgcgt cggtcttggc ccacagcctc
     3361 gtcgccaagg tgtcggtgcc gatcggacgc aacagcaacg tggccggaag ctccttcatc
     3421 acggcgatgc acaccagggc ggcggccaca ccagctccgg gcgcggccaa cggcaatgtg
     3481 acggcaagcc aggcccgcag cggcccgcgt cccgacgagc gggcgacgtc gtccaactcg
     3541 ggtgcgactt gagccagcgc cgtgtgcagc gcgccgatcg cgagcgagag gaacaccacc
     3601 acgtaggcgg cgatcaacat cggcatccgt tgatacagcg gcgtcgcgac gcgcaccccg
     3661 aagaacacca gcgacaggcc gacgacgatg ccggggagcg catgcccggc ataggcgacg
     3721 gtggtcacgc cattggccaa cccgccgggg tggcgagccg acagcatcgc cacgggcagc
     3781 gcgacgacca ccgtggcggc ggcacccaac acacccagca acagcgtggt gcgggccgcg
     3841 gcgaacacgt cgctcagnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc cgtgcagccc gccacagcgg gccaccgcca
     3961 cagcccgagc cgcaccgttg ccgcgtcatg accgacgccg ctaccgacac gagcgatgcg
     4021 gccggccgcg ctcgaacgaa cccccaacca cagtggcacc aacgtaatcg ccactaacac
     4081 acagccgagc accgctgccg gcgtgcggtc gaacgaggct cggtacgacg tgtagatcac
     4141 cttggtcagt gtctcgtagc gcatgatcga caccgcgccg aactcgctca acgtgtacag
     4201 cgcgaccagc acgcaaccgc cgaaggccgc gatcctgatc tggcgcaatg tgacaccgac
     4261 aaagacctgc gccggagaac gcccagcact gcgggcgact tcggccaatg ccgggtcggc
     4321 gcgtcgcaac gcccccagca ccgggaggta gacgtacgga tacgagatca gcgtcaacac
     4381 cagcaccgag ccacgaaacc cggccaggct cgggctccag ccgatccacc cccacgccgc
     4441 gatgtacgac gggatcgcga gcggcatcgc caacgcgaca cgccagaacg cgcgcagcgg
     4501 cagatcggcg cggnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4621 nnnnnnnnnn cacgaaccgg tcccacccct ggtcggtggc ccggatgccg atgtagacga
     4681 tcggcagcaa cacgaccacg gacgcgacga cgcctcccac caacaacggg gtcggtggga
     4741 gacgtcggcg cgcaacaggc cggtcgagcc gaatcagctc agcaagccca gatcggtgag
     4801 cagtgcctgc gtatcggcca gcgagtcaag atcggtcagg tcgagcgccg gaccctgcaa
     4861 ctcgtcgct
//
LOCUS       DAORNF010000168         4864 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_12971_length_4864_cov_1.396132, whole genome shotgun sequence.
ACCESSION   DAORNF010000168 DAORNF010000000
VERSION     DAORNF010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4864
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12971_length_4864_cov_1.396132"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>250
                     /locus_tag="PLV68_07950"
     CDS             <1..>250
                     /locus_tag="PLV68_07950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01220.1"
                     /translation="ANDLIAPQTAFIHGVGLLAPDFALMAARNSSLIWSPRTNISLYG
                     ETARVSVAHRFGVRIALGTDWIASGSANISNANNPNATL"
     assembly_gap    251..429
                     /estimated_length=179
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <430..>1575
                     /locus_tag="PLV68_07955"
     CDS             <430..>1575
                     /locus_tag="PLV68_07955"
                     /inference="COORDINATES: protein motif:HMM:NF014466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HRE01221.1"
                     /translation="KDVALVMRGGKTLYGDDNVVAGLRLSGCDAFEMCGAFKQVCVQD
                     EVGKSWPAFAAENADIYPAYFCGPPPGEPTCKPSRPKAVNNSTIYTGDITNSDLDGDG
                     IDNRSDHCPLDDGRQADGDGDGVGDSCDVCPLAADVTTCPAFDPKDRDGDGDVDSADN
                     CPGHANANQADADGDGKGDVCDDCPMTANPGAGACPGSIYGVKEGTLLPDIQVRLAGA
                     LVTGKGSNGFFLQVKETDAGYKGANYSGIFVFTTNATFLAQAEIGGRVNVEGQITNFF
                     GQLELSNVASVSRVRTTTEASPAPTMVTIDEARVGGSRAVALESVLVRLGSSTVTAIN
                     SGTGEFTATQGQVSLPVDDFVFLTNFFPPVGQAYSTLTGILALRSSS"
     assembly_gap    1576..1715
                     /estimated_length=140
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1716..>2016
                     /locus_tag="PLV68_07960"
     CDS             <1716..>2016
                     /locus_tag="PLV68_07960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01222.1"
                     /translation="VRLTMPVATDTTITVTSGDTSALTVDTPSLTIPAGQVAAEVRLT
                     GVAASPAVTLTATLDDVSVTSSVRVLTDTDQPTALTLTPTSSTINPGATVTYTVTL"
     assembly_gap    2017..2157
                     /estimated_length=141
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2158..2888
                     /locus_tag="PLV68_07965"
     CDS             <2158..2888
                     /locus_tag="PLV68_07965"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HRE01223.1"
                     /translation="GTTITASYNSASATARINVIEPPTGLVINEIDYDQPGTDMDEFV
                     EIYNGTGAAVDFTNLAIAFVNGSSAASNQPNLQYMQASLAGVGMLPAGSYLLVTKQGL
                     VTDPGLNCAGPGVGTTCILFTPTVFADNPSNPPNQPPTKPTAWPGSGIQNGDPDGVLL
                     LNTSTGRVIDRLSYGGPMTTCKVTNIGGEISLVEGTALDPTVRDVVTGSLGRNELGRD
                     TNDANTDWKFSATPSPGKANPAFQ"
     gene            2995..>3286
                     /locus_tag="PLV68_07970"
     CDS             2995..>3286
                     /locus_tag="PLV68_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01224.1"
                     /translation="MTSQRLLKILDPLGHLEPSPAEGPIAVVWAPSPPLGLVIRNRLL
                     AARFHPLMATSFRHVASSMMPGAKPQAELVILDLDATSASDLSSLASARWGGF"
     assembly_gap    3287..3351
                     /estimated_length=65
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3520..>4500
                     /locus_tag="PLV68_07975"
     CDS             3520..>4500
                     /locus_tag="PLV68_07975"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HRE01225.1"
                     /translation="MAFSLDKQGISVQEIHRNASPAFLYEAALRFEKGSAISSTGSLI
                     AYSGKKTGRSPTDKRIVDEPEVRDDIWWGNVNIKLDPQSFLINRERAIDYLNTRERLY
                     VVDGYAGWDPAFRLRIRIVCARAYHALFMHNMLIRPTPAELEQFGDPDYVIFNAGGFP
                     ANKHTAGMTSSTSVDLSFSRREFVILGTEYAGEMKKGVFTIMNYLMPKQDVLSMHCSA
                     TMARDGSDTSILFGLSGTGKTTLSADPKRLLIGDDEHCWSNHGVFNIEGGCYAKVIDL
                     SREQEPDIWNALQFGSVLENVVFEPFSHAVDYKDISITQNTRGCYPLDSIP"
     assembly_gap    4501..4531
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4532..>4864
                     /locus_tag="PLV68_07980"
     CDS             <4532..>4864
                     /locus_tag="PLV68_07980"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: protein motif:HMM:NF013461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HRE01226.1"
                     /translation="KNVIFLTADAFGVLPPVAKLSPGQAMYHFISGYTAKVAGTEVGV
                     KDPEATFSPCFGGPFLVWHPTKYAEMLAEKLRKHGAQTWLVNTGWSGGPYGVGSRIKL
                     RYTRAIIDA"
BASE COUNT          745 a         1629 c         1277 g          657 t
ORIGIN      
        1 gcgaacgatc tcatcgcgcc gcagaccgcg ttcatccacg gcgtcggcct gctcgcccca
       61 gactttgccc tgatggcagc ccgcaacagc tcgctcatct ggtcgccgcg caccaacatc
      121 tcgctctacg gcgagaccgc gcgggtcagc gtggcccacc gcttcggggt gcgcatcgcc
      181 ctgggcactg actggatcgc cagcggctcg gccaacatca gcaacgccaa caacccgaac
      241 gccacgctca nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnc caaggatgtg gcgctggtca tgcgcggtgg caagaccctc tacggcgacg
      481 acaatgtggt cgccggcctg cgcctctccg gctgtgatgc cttcgagatg tgcggcgcct
      541 tcaaacaagt gtgcgtgcag gacgaggtcg gcaagagctg gcccgcgttc gccgctgaga
      601 acgccgacat ctatccggcc tacttctgcg gcccgccgcc gggcgagccg acctgcaagc
      661 ccagccgccc caaggcggtc aacaactcca ccatctacac cggcgacatc accaactcgg
      721 acctcgatgg cgacggcatc gacaaccgca gcgaccactg cccgctcgac gacggccgtc
      781 aggccgatgg cgatggcgac ggcgtgggcg actcgtgcga cgtgtgcccg ctggctgccg
      841 acgtcaccac ctgcccggcc ttcgatccca aggatcgcga tggcgatggc gatgtggact
      901 ccgccgacaa ctgcccgggc cacgccaacg ccaaccaggc cgacgccgac ggcgacggca
      961 agggcgacgt ctgcgacgac tgcccgatga ccgccaaccc cggcgccggc gcttgccctg
     1021 gctccatcta cggcgtcaag gaagggaccc tcttacccga tatccaggtc cggctcgccg
     1081 gcgcgctggt caccggcaag ggttccaacg gcttcttcct ccaggtcaag gagaccgacg
     1141 cgggatacaa gggcgccaac tactccggca tcttcgtgtt caccaccaac gctaccttcc
     1201 tggcccaggc cgagatcggc gggcgcgtga acgtcgaggg ccagatcacc aacttcttcg
     1261 ggcagctcga gctgtccaac gtcgcctcgg tcagccgcgt ccgcaccacc accgaggcga
     1321 gcccggcgcc caccatggtc accatcgacg aggcccgcgt cggcggctct cgcgcggtcg
     1381 cgctcgagtc ggtgctggtg cgcctgggca gctccaccgt gaccgccatc aacagcggca
     1441 ccggcgagtt caccgccacg cagggccagg tctcgctgcc ggtggacgat ttcgtgttcc
     1501 tcaccaactt cttcccgccg gtcggccagg cctactccac gctcaccggc atcctggccc
     1561 tgcgcagcag ctccannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngtgcg cctgaccatg ccggtggcca
     1741 ccgacaccac catcaccgtc acctcgggag acaccagcgc actgaccgtg gataccccgt
     1801 cgctcacgat cccggcggga caggtcgccg ccgaggtccg cctcaccggc gtggccgcct
     1861 cgccggccgt gaccctcacc gccacgctcg acgacgtgag cgtcaccagc tcggtgcggg
     1921 tcttgaccga caccgaccag ccgaccgcgc tcaccttgac cccgacctcc agcaccatca
     1981 accccggcgc caccgtcacc tacaccgtga ccctcannnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnccg
     2161 gcaccaccat caccgcgagc tacaacagcg ccagcgccac cgcgcgcatc aacgtgatcg
     2221 agccgccgac gggcctggtg atcaacgaga tcgactacga ccagcccggc accgatatgg
     2281 atgagttcgt cgagatctac aacggcaccg gggccgctgt ggacttcacc aacctggcca
     2341 tcgccttcgt caacggcagc tctgccgcgt cgaaccagcc caacctccag tacatgcagg
     2401 cctcgctggc cggcgtcggc atgctgccgg ccggctcgta cctgctggta acaaagcagg
     2461 gtctggtcac cgatccgggc ctcaactgcg cgggtcccgg cgtcggcacc acctgcatcc
     2521 tgttcacgcc caccgtgttc gccgacaacc cgtcgaaccc gcccaaccag ccgccgacca
     2581 agcccaccgc gtggcccggc agcggcatcc agaacggcga tcccgacggc gtgctgctgc
     2641 tcaacacctc cacgggccgg gtgatcgacc gcctgtccta cggcggtccg atgaccacct
     2701 gcaaggtcac caacatcggc ggcgagatct cgctggtcga aggcacggcg ctcgatccga
     2761 cggtgcgcga cgtggtcacc ggctcgctcg gccgcaacga gctgggccgc gacaccaacg
     2821 acgccaacac cgactggaag ttctccgcga ctcccagccc gggcaaggcc aacccggcgt
     2881 tccagtaagg cgacctctcg cttcgccacc tcggcgcgcc ggtctggcgc gccgagcgcg
     2941 ttttcggctg gccacacgcc tgttcggcct ccgccccagg tcgagtctgg cagtgtgacc
     3001 tcccagcgcc tgctcaagat cctcgacccg ctcggccacc tcgagccatc gccggcggag
     3061 ggccccatcg cggtggtctg ggcgccgagc ccaccgctgg gcctggtcat cagaaatcga
     3121 ctgcttgcag cgcggtttca cccactgatg gccacctctt tccgccacgt cgcgtcaagc
     3181 atgatgccgg gcgccaagcc ccaggcggag ctggtcatcc tggatctcga cgcgacctcg
     3241 gcctctgacc tgagctcgct ggcctccgcg cgctggggcg gcttcannnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngagctggaa
     3361 gcagtgatcc cgcgtcgacg agcctccacc gcgatgcgcg acgtgctcga gcgctggcgg
     3421 atgggccacg cgtagccccc ggcggccgga agcgggcgca cgctccgttt tgggtgtata
     3481 ccaggcgcac ctaacgccta ctcacccgac ggagacaaca tggccttctc gctcgacaag
     3541 caaggcatct cggtacaaga aatccatcgc aacgcctcgc cggcgtttct gtacgaagcg
     3601 gcgctccgct tcgagaaggg ctcggcgatc tcctcgaccg gatcgctcat cgcctactcc
     3661 ggcaagaaga ccggtcgcag ccccaccgac aagcgcatcg tggacgagcc cgaggtgcgc
     3721 gacgacatct ggtggggcaa cgtcaacatc aagctcgatc cgcagagctt cctcatcaac
     3781 cgcgagcggg ccatcgacta cctgaacacg cgcgagcgcc tgtacgtggt ggatggctac
     3841 gccggctggg atccggcctt ccgcctgcgc atccgcatcg tgtgcgcgcg cgcctatcac
     3901 gcgctgttca tgcacaacat gctgatccgc cccacccccg cggagctcga gcagttcggc
     3961 gatccggact acgtcatctt caacgccggt ggcttccccg ccaacaagca caccgccggc
     4021 atgacctcca gcaccagcgt ggatctgtcc ttctcgcgcc gcgagttcgt catcctcggc
     4081 accgagtacg ccggcgagat gaaaaagggc gtgttcacca tcatgaacta cctgatgccc
     4141 aagcaggacg tgctgtcgat gcactgctcg gccaccatgg cgcgcgacgg cagcgacacg
     4201 tcgatcctct ttggcctctc gggcaccggc aagaccacgc tgtcggcaga tcccaagcgc
     4261 ctgctcatcg gcgacgatga gcactgctgg agcaaccacg gcgtcttcaa catcgagggt
     4321 ggctgctacg ccaaggtcat cgatctttcc cgcgagcaag agccggacat ctggaacgcc
     4381 ctgcagttcg gctcggtgct ggagaacgtg gtgttcgagc cgttctcgca cgccgtcgac
     4441 tacaaggaca tctcgatcac ccagaacacg cgcggctgct acccgctcga ctccatcccc
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn naagaacgtc atcttcttga cggcggatgc
     4561 cttcggcgtg ctgccgccgg tggccaagct ctctccgggc caggccatgt accacttcat
     4621 cagcggctac accgccaagg tcgctggtac cgaggtcggc gtgaaggatc ccgaggccac
     4681 gttctcgccg tgcttcggcg gcccgttcct ggtctggcac cccaccaagt acgccgagat
     4741 gctggccgag aagctgcgca agcacggcgc gcagacctgg ctggtgaaca ccggctggtc
     4801 gggcggcccg tatggcgtgg gctcgcgcat caagctgcgc tacacgcgcg ccatcatcga
     4861 tgcc
//
LOCUS       DAORNF010000169         4854 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_13006_length_4854_cov_1.790373, whole genome shotgun sequence.
ACCESSION   DAORNF010000169 DAORNF010000000
VERSION     DAORNF010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4854
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13006_length_4854_cov_1.790373"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>298
                     /locus_tag="PLV68_07985"
     CDS             <1..>298
                     /locus_tag="PLV68_07985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01227.1"
                     /translation="VFSGSVTGRLALFGHRITQTTFDVRLANGVLEMSLPSSRAATLD
                     LGFIDFRVSGTARSDGTFSFTGSLSTSGNVFGVVSWSGSVAVTVANGGIFGTYSG"
     assembly_gap    299..379
                     /estimated_length=81
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            416..>777
                     /locus_tag="PLV68_07990"
     CDS             416..>777
                     /locus_tag="PLV68_07990"
                     /inference="COORDINATES: protein motif:HMM:NF014544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HYR domain-containing protein"
                     /protein_id="HRE01228.1"
                     /translation="MCGPFGCTAIQESVTYAFDLAKNPPAPDTTPPVMAQPGNLSIAT
                     SQSSGSIPVSYTQPTASDNRDGTISARCTPGSGANFTIGATTTVTCTARDAAGNTTTR
                     SFTITVAFSPASVLLSGGV"
     assembly_gap    778..1009
                     /estimated_length=232
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1599..2339)
                     /locus_tag="PLV68_07995"
     CDS             complement(1599..2339)
                     /locus_tag="PLV68_07995"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRE01229.1"
                     /translation="MADYTFLTYDVRDHVAHVEFGASGYAKSQEWELFDVLHRVNTDD
                     DVHVMVLTSAGEKFGGGNHHEDDPFNGGNYYERAIKLFSTWMRVSKPIVVALSGPGSL
                     TLALLSDIVVAERHVDIRDTHVVLGVPAATGAFLWPQSVGLAKAKRHLLVGDGIRAEE
                     AVSMGLIAEVVDTGAGAARARELAAGIATLNPTGVQMSKRALNAWMEQNFNPILQHGL
                     GLEFLHFPAGLFDGSATASKETTDGSEG"
     gene            2610..>2923
                     /locus_tag="PLV68_08000"
     CDS             2610..>2923
                     /locus_tag="PLV68_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HRE01230.1"
                     /translation="MSADDHVTAPPNVWSDRLPSKFKDVGPRVVRELASVNPKNHLGE
                     LVFETGEKLVDFWCYEDVKMPLMKGENAVGFEIDERESVPMTYDEIRLGCYDQKARLA
                     DM"
     assembly_gap    2924..2938
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2939..3788
                     /locus_tag="PLV68_08005"
     CDS             <2939..3788
                     /locus_tag="PLV68_08005"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRE01231.1"
                     /translation="DQAGVEASLCFPNFFPRFGGQYFVDGKDKELALLCVMAYNDWML
                     EEWCAGTDGRLVPLGILPGWDVELCAAEADRVGPLGMRAFTFSEAPARVGLPSIASGE
                     WDPFFAACERNQIVVAMHIGSAPPISPSLDTTPGTQSALLACNSASAMLDWICHGVFE
                     RFPNLQALLAESNIGWIPYFLERADHVWEHNRAWNGFRAACPNPPSSYFPNHIYATFF
                     DDEYGLTHAEEIGVDNILFEMDYPHGDTNWPDSHDFAHRLTAGLSPENQQKILRGNAR
                     KLLRLD"
     assembly_gap    3973..3987
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3988..4249
                     /locus_tag="PLV68_08010"
     CDS             <3988..4249
                     /locus_tag="PLV68_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HRE01232.1"
                     /translation="QLAVTGELRTFEDRGEHGDAVYVYRRFCPDCGSPIVSELVSPPG
                     VVAVKAGTLDDRSEIVPAAEIWCETRQPWAQLPGMPVSLVRE"
     gene            4255..>4854
                     /locus_tag="PLV68_08015"
     CDS             4255..>4854
                     /locus_tag="PLV68_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRE01233.1"
                     /translation="MADERTGIEGINGVHHLAISTKDMKGQLEFFTDVLGAELKALYW
                     MHGVADTFHGFVKLNDTCYVAFVQGPVNATIEPVLGVSHSGSTAGPSAPGTMQHVAFN
                     VKDLDELLVMRDRIRSRGVNVFGPLDHGMCHSIYFAGPELLSLEIATSERPIDARAWI
                     DPEVVDLVGIDDQMLQRLKSPAAYAGEGGAVPNPPIDWSK"
BASE COUNT          752 a         1474 c         1453 g          832 t
ORIGIN      
        1 cgtgttcagc ggttcggtca ccggacgtct cgcgctgttc ggtcaccgga tcacgcagac
       61 cacgttcgac gtccgcctcg ccaacggtgt actggagatg tcgctgccct cgtcccgagc
      121 agccacgctc gacctcggct tcatcgactt ccgcgtgtcg ggcacggccc gcagcgacgg
      181 gacgttctcg ttcaccgggt cgttgtccac cagcggcaac gtgttcggtg tcgtgtcgtg
      241 gagcggctcg gtcgcggtga cggtcgccaa cggcggcatc ttcggcacct actcgggtnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnng atctcaatct cgacggtcga acgacgggat tcacggtgtg
      421 cggcccgttc ggctgcacgg cgatccaaga gtcggtcacc tatgcgttcg atctggccaa
      481 gaacccgccc gcgccggata ccaccccacc cgtcatggcc cagccgggaa acctctcgat
      541 cgccacgtcg caatcgagcg gctcgattcc cgtgagctac acgcagccga ccgcgagcga
      601 caaccgcgac ggcacgatca gcgcacgctg cactccgggg tccggcgcca acttcacgat
      661 cggcgccacg accactgtca cctgcacagc ccgcgacgcc gctggcaaca cgacgacgcg
      721 cagcttcacg atcaccgtcg cgttctcgcc agcgagtgtg ctgctctccg gtggcgtnnn
      781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc gagagcgtcc
     1021 gtgtcgacgt cccaggcggc accctgcccg agaccggcac cgccccgctc gccctcacca
     1081 ccacagccct tgcgaccctc ctcctcggtc tcaccctcct cgtcaccacc accacccgcc
     1141 cccgccgccg attgaactga cgaacgaact cagtcgtgcg gcacgaccgc cgcattgctt
     1201 cagctgttgt gtcgtgccgt ccatgcggtc cacagcgcgg catagatggg acgactatct
     1261 gatgttctgc tcgatcagcg cgcgctctgg cccgcatccc gacgactacg acccccgaca
     1321 catcctcgac ttgttctccg aacaggtcgc cacacagcgc gcgctgacgg tgccgatgcc
     1381 caccgaccca cgtgcgcgtg cggccgcgat ggactacttg cgtcgcatcg acgtgtccgg
     1441 cgaagcaggc gtcgtcgatg tgcccgtcga gatctcgcga gtgctccgcg acgagactgg
     1501 catgacgctt tcgcggtggg gttacgcggc gcgcatgcgg tggcccgtga cctactcgtc
     1561 ggcggtgcga gggccagcac ggtcgcccgc cgcgtcggct acccctccga cccgtcggtg
     1621 gtctccttgc tggcggtcgc cgagccgtcg aagaggccgg cggggaagtg gaggaattcg
     1681 agtccgaggc cgtgttggag gatgggattg aagttctgtt ccatccacgc gttgagtgcc
     1741 cgcttgctca tctgcactcc ggtcgggttg agcgtggcga tccctgcggc tagttcgcgt
     1801 gcacgtgcgg caccggcacc ggtgtcgacc acctccgcga tcaggcccat cgacaccgct
     1861 tcttcggctc tgatcccgtc gccgacgagc aggtgtcgct tggccttggc gagtccgacc
     1921 gactggggcc agaggaaggc accggtggcg gccgggacgc cgagcacgac gtgtgtgtcg
     1981 cggatgtcga cgtggcgttc ggcgacgacg atgtcgctga gcagcgccag cgtcaggctg
     2041 cctggcccgc tgagtgcgac gacgatcggt ttgctgaccc gcatccacgt gctgaagagc
     2101 ttgatcgctc gctcgtagta gttgccgccg ttgaacgggt cgtcttcgtg gtggttgccc
     2161 ccgccgaact tttcgccggc gctcgtgagg accatgacgt gtacgtcgtc gtcggtgttg
     2221 acgcggtgga ggacgtcgaa cagttcccac tcttgcgact tggcgtagcc actggcgccg
     2281 aactcgacgt gtgcgacgtg atctcggacg tcgtaagtga ggaacgtgta gtcggccatc
     2341 ccagccccct gatgtgatgc cgaggcctcg tgcccggcgg cggtgcgatc ttggcatcga
     2401 tctcattcgc ccgtcaatgg acaaacaaga ggttggtaga acaactaggg gctgtgcgac
     2461 gttccgagga cagcgcgctg ggccgccaat gaaccccaca ccttgcgatg ggagggactc
     2521 gacgtatacc ctcgcttgaa cgaccgtttg gtcgacccga ccaactccgg aggatcgaaa
     2581 tgcttaccgc cgagaagctg cctggactcg tgtccgctga cgatcacgtc accgcgccac
     2641 cgaacgtctg gagtgaccgc ctccccagca agttcaagga cgtcgggccc cgcgtcgtgc
     2701 gcgagttggc atctgtcaac ccgaagaacc accttggcga gctggtcttc gagaccggcg
     2761 agaagctcgt cgacttctgg tgctacgaag acgtcaagat gccgctgatg aagggcgaga
     2821 acgcggtcgg cttcgagatc gacgaacgtg agagcgttcc gatgacctac gacgagatcc
     2881 ggctcggctg ctacgaccag aaggcgcggc tcgcggacat ggannnnnnn nnnnnnnngg
     2941 accaagcggg ggtcgaagcg agtctctgct tcccgaactt cttcccgcgc ttcggcgggc
     3001 agtacttcgt cgacggcaag gacaaggagc tggccttgct gtgcgtcatg gcctacaacg
     3061 attggatgtt ggaagagtgg tgtgcgggaa ccgacgggcg gcttgtgccg ctgggcatcc
     3121 ttccgggctg ggacgtcgaa ctgtgcgccg ccgaggccga ccgcgttggt ccgctgggca
     3181 tgcgtgcctt cacgttctcc gaagcgccgg cccgtgtcgg cctgccgtcg atcgcatctg
     3241 gcgagtggga tccattcttc gcggcatgtg agcggaacca aatcgtcgtc gcgatgcaca
     3301 tcggatcagc tccgccgatc agcccgtcgc tcgacacgac gccgggtacg cagtcggcac
     3361 tgctcgcttg caactcggca agtgccatgc tcgattggat ctgccacggg gtcttcgagc
     3421 ggttccccaa cctgcaagcg ttgctggccg agtcgaacat cggttggatt ccgtacttcc
     3481 tcgaacgtgc cgatcacgtg tgggagcaca accgcgcctg gaacggtttc cgggccgcat
     3541 gcccgaaccc accgagcagt tactttccga accacatcta cgccaccttc ttcgacgacg
     3601 agtatggact cacgcacgcc gaggagatcg gggtcgacaa catcctgttc gagatggatt
     3661 atccgcacgg ggacacgaac tggcctgact cgcacgattt cgcgcacagg ctgaccgctg
     3721 gcctctcgcc agagaaccag cagaagatcc tgcgcggcaa cgcccgcaag ctgcttcgcc
     3781 tcgactgagc ccgacccgtt gggtctggag gcgtcggagt agcgtgccgc catggcccga
     3841 accggacgat gcctctgcgg caacattggc tatgagatca gtggagacct catctccacc
     3901 gtggtctgtc actgcgagca ctgtcagcga cagagcggcg gcgcgttctc cgtcaacctc
     3961 atcgcgcacg tcnnnnnnnn nnnnnnncca gctcgcagtc accggtgagc tccgcacgtt
     4021 cgaagaccgc ggcgagcacg gcgatgcggt gtacgtctac cgacgctttt gcccggactg
     4081 cggttcgccg atcgtgtcgg aactggtctc gccgcccggc gtcgtggcgg tgaaggcggg
     4141 gacgttggat gaccgcagcg agatcgttcc cgcggccgag atctggtgcg agacccgtca
     4201 accttgggca cagcttcccg gtatgcccgt ctcgctcgtc agggagtgac acgcatggct
     4261 gacgagagaa caggcatcga gggcatcaac ggggttcatc acttggcgat ctccaccaag
     4321 gatatgaagg ggcagttgga gttcttcacc gacgtgctgg gcgcggagct caaggcgttg
     4381 tattggatgc atggcgtggc ggacacgttc cacggcttcg tcaagttgaa cgacacgtgc
     4441 tacgtggcgt tcgtgcaagg tccggtgaac gcaaccatcg agcccgtctt gggggtgtcg
     4501 cattcgggga gcacggctgg tccgtcggcg ccgggcacga tgcagcacgt cgcgttcaac
     4561 gtgaaggacc ttgacgaact gctggtgatg cgcgaccgga tccgcagccg cggggtcaac
     4621 gtgttcggtc cactggacca cggcatgtgc cactcgatct acttcgcggg tccggagttg
     4681 ttgtcgttgg agatcgctac gtcggagcgg ccgatcgacg cgcgggcctg gatcgatccg
     4741 gaggttgtcg atctggtcgg catcgacgat cagatgttgc agcggttgaa gtcacccgct
     4801 gcatatgccg gcgagggtgg tgccgtgccg aacccaccga tcgactggtc caag
//
LOCUS       DAORNF010000170         4831 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_13093_length_4831_cov_1.677973, whole genome shotgun sequence.
ACCESSION   DAORNF010000170 DAORNF010000000
VERSION     DAORNF010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4831
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13093_length_4831_cov_1.677973"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>281)
                     /locus_tag="PLV68_08020"
     CDS             complement(<1..>281)
                     /locus_tag="PLV68_08020"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01234.1"
                     /translation="SWISPEMDQVLRYGVHGVWDGADEATWAKAYHLDHDAVWAARAA
                     GRRRLVSYVESRMGDGILDPDALTIGFARRFATYKRATLLLSRPERLKRL"
     assembly_gap    282..355
                     /estimated_length=74
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<356..>1220)
                     /gene="glgP"
                     /locus_tag="PLV68_08025"
     CDS             complement(<356..>1220)
                     /gene="glgP"
                     /locus_tag="PLV68_08025"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HRE01235.1"
                     /translation="EVALTSDRWFQTKPGQTGPGDSPLGLVAYFSPEFGISETVPQYS
                     GGLGVLAGDHLKASSDLGVPLVAIGLLYAEGYFRQSLDADGWQQERYPLLDRAGMALT
                     HTGIEVSIDLAGDHVPVAVWRADVGRIPMYLLDAPGVTDRLYGGDVEHRLRQEMVLGI
                     AGVRALRALGLEPQVFHTNEGHAGFLERIRELVHSGLSINEAIEASRAGGVFTTHTPV
                     PAGIDRFPHALIDKYFGEFAASIGTTVERLAAIGHRPDEPDDDRFNMAVMGLRLAARA
                     NGVAALHGAVSR"
     assembly_gap    1221..1236
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1237..1479)
                     /locus_tag="PLV68_08030"
     CDS             complement(<1237..1479)
                     /locus_tag="PLV68_08030"
                     /inference="COORDINATES: protein motif:HMM:NF023325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3417 domain-containing protein"
                     /protein_id="HRE01236.1"
                     /translation="MRSVRQFSVVPAVPAPLAGLNTLANNLHWCWDRELQQLFADLHP
                     EAWEASGHQPLATLARISPAEIVAFAGNIRVLGSYPV"
     gene            complement(1571..2758)
                     /locus_tag="PLV68_08035"
     CDS             complement(1571..2758)
                     /locus_tag="PLV68_08035"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRE01237.1"
                     /translation="MRVLMLSWEYPPLVVGGIAAHVDGLARALVRAGHEVVVFSLATP
                     DCDDDTATDGVRVVRANIDLPWLPDDNLIGQMASANHHLTQLVTSLGDWRPDIVHAHD
                     WLVAWAGDTIATLTGAPLVATIHATERGRHGGHLPHGTPAAINSIEWWLTYQARSVIC
                     CSNFMVRELLNHFELPTDKVHRVPNGVDLDVWAPPSGGAGALGGGAALDPGEASLVVA
                     WGRVQYEKGFQVLVRAIGALRDRGTDAPCIIAGRGSYLAELQSLIDTEGLGDRVQLAG
                     FVADDELRALLHRAAVAVIPSLYEPFGIVALEAMAAGVPTVVAATGGLAETVRDTGAG
                     LLFEPGNHHDLATQIGTVLDDPTVSRSLRSRAADVIGQYTWDAIAASTAAVYDATLHI
                     DAT"
     gene            2801..>3130
                     /locus_tag="PLV68_08040"
     CDS             2801..>3130
                     /locus_tag="PLV68_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01238.1"
                     /translation="MASERPVADWPGELDVHLFGEGRHRRLWQLLGAHRLEPAGGAER
                     GTRFAVWAPNAAQVWVVGDWNQWGDGTAMHPVAGSGMWCASVAAARPGHTYKYAVVDS
                     AGRTVLKA"
     assembly_gap    3131..3140
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3141..>3582
                     /locus_tag="PLV68_08045"
     CDS             <3141..>3582
                     /locus_tag="PLV68_08045"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HRE01239.1"
                     /translation="MPRQTECPPGPSSVVPAETAYVWQDDAWIERRSNRPSAPLRIYE
                     VHLPSWRHGVGNYVELGHQLADHVTALGFTHVELLPVAEHPFGGSWGYQVSGYYAPTA
                     RLGTPDQFREFVDILHRRGVGVILDWVPAHFPKDEWALARFDGTA"
     assembly_gap    3583..3725
                     /estimated_length=143
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3726..>4460
                     /locus_tag="PLV68_08050"
     CDS             <3726..>4460
                     /locus_tag="PLV68_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HRE01240.1"
                     /translation="FHIDGLRVDAVASMLYLDYSREPGQWVPNRFGGRENLGAIELLR
                     EVNTVVGDEVPTAMMIAEESTSWPKVTHPVHLGGLGFSHKWNMGWMHDTLGYFERDTI
                     HRQWHHNDLTFGLLYAFGERYVLPLSHDEVVHGKASLLGKMPGDDWQRFANLRALYAW
                     MWALPGAPLLFMGAELAPFSEWNEAAGLPWHLLDHAPHRGVRDLVATLNAVAAEATEL
                     WAGDTDSSSFQWLDADDALQGARPPDT"
     assembly_gap    4461..4599
                     /estimated_length=139
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          662 a         1482 c         1625 g          680 t
ORIGIN      
        1 aggcgcttca gccgctcggg ccgcgacagc aacagcgtcg cccgcttgta ggtggcaaag
       61 cgccgggcga agccgatcgt caaggcgtcg ggatcgagga tgccatcgcc catgcggctc
      121 tcgacgtagc tgaccaggcg ccgcctgcct gcggcccggg cggcccagac cgcgtcgtgg
      181 tcgaggtggt aggccttggc ccaggtggcc tcgtcggcgc cgtcccacac accgtgcacg
      241 ccgtagcgca gcacctggtc catctcgggg ctgatccagc tnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncgcgg
      361 ctgacggcgc cgtgcaacgc ggccacgccg ttggcccggg cggcgaggcg caggcccatc
      421 accgccatgt tgaagcggtc gtcgtcgggc tcgtcggggc gatggccgat cgccgcgagt
      481 cgttcgacgg tggtgccgat cgacgccgcg aactcgccga agtatttgtc gatcagtgcg
      541 tggggaaacc ggtcgatgcc cgccggcacc ggcgtgtggg tggtgaacac gccgccggcc
      601 cgcgaggcct cgatcgcctc gttgatcgac agaccggagt ggaccagttc gcggatgcgc
      661 tcgaggaacc cggcgtggcc ttcgttggtg tggaacacct gcggctcgag acccagagcg
      721 cgcagggcgc gcaccccggc gatgccgagc accatctctt ggcgcaggcg atgctcgacg
      781 tcgccgccgt agagccggtc ggtgacgccg ggtgcgtcga gcaggtacat cgggatgcgg
      841 ccgacgtcgg cccgccacac ggcgaccggc acatggtcgc cagccaggtc gatcgagacc
      901 tcgatgccgg tgtgagtcag ggccatgccc gcccggtcga gcagcgggta gcgctcttgt
      961 tgccaaccgt cggcgtcgag gctctggcgg aagtagccct cggcgtacag cagcccgatc
     1021 gccaccagcg gcacgccgag atcggacgac gccttgaggt ggtcaccggc gagcacgcca
     1081 aggccgccgc tgtactgagg aacggtctcg ctgatgccga actcgggcga gaagtaggcc
     1141 accagcccga gcggcgagtc acccggtccg gtctggcccg gtttggtctg gaaccagcgg
     1201 tcggaggtca gcgccacctc nnnnnnnnnn nnnnnngacc ggataggaac ccagcacccg
     1261 gatattgccc gcgaaggcga cgatctcggc gggcgagatg cgcgccaacg tcgccagcgg
     1321 ctggtggccg gacgcctccc atgcttcggg atggaggtcg gcgaacagtt gctgcagctc
     1381 gcggtcccag caccagtgga ggttgttggc cagtgtgttc agccctgcga gaggggcggg
     1441 cacggcggga acgacgctga actggcggac gctgcgcatg gggcacgagg gtaccccggg
     1501 ggtctgacgg accggtgagc gtgacgatga cacctctgtg aagtgttccc cgagggacgc
     1561 tgaagccgtg ttacgtcgcg tcgatgtgca gcgtcgcgtc gtagaccgcg gcggtcgagg
     1621 cggcgatcgc gtcccatgtg tactggccga tcacgtccgc ggctcgcgag cgcagcgatc
     1681 gtgacacggt ggggtcgtcg agcaccgtgc cgatctgggt tgcgagatcg tggtggttgc
     1741 cgggttcgaa cagcagcccg gcaccggtgt cgcgcacggt ctcggccaat ccgccggtgg
     1801 cggcgacgac ggtcggcacg cctgcagcca tcgcctccag ggcgacgatg ccgaacggtt
     1861 cgtacagcga ggggatcacc gcgaccgcgg ctcggtgcag caaggcgcgc agttcgtcgt
     1921 cggccacgaa cccggcgagt tggacgcggt cacccagccc ttcggtgtcg atcaggctct
     1981 gcagttcggc gaggtagctg ccccgcccgg cgatgatgca gggggcatcg gtgccgcggt
     2041 cgcgcagcgc gccgatggcg cgcaccaaca cttgaaaccc cttctcatac tgcacccgcc
     2101 cccacgcgac caccagcgac gcctcgcccg ggtcgagcgc ggccccgcca ccgagcgcgc
     2161 cagcgccacc gctcggcggt gcccacacgt cgaggtcgac cccgttgggc acccggtgca
     2221 ccttgtcggt cggcagctcg aagtggttga gcagctcgcg caccatgaag ttgctgcaac
     2281 agatcaccga gcgggcctga tatgtgagcc accactcgat cgagttgatc gctgcagggg
     2341 tgccgtgggg caggtgaccg ccgtggcgcc cgcgctcggt ggcgtgaatg gtggccacca
     2401 gcggcgcccc ggtcagcgtg gcgatggtgt cgcccgccca ggcgaccagc cagtcgtggg
     2461 catggacgat gtcgggccgc cagtcgccca ggctggtgac gagttgggtc aggtggtggt
     2521 tggccgaggc catctggccg atcaggttgt cgtcggggag ccacggcaga tcgatgttgg
     2581 cgcggaccac gcgcacgccg tcggtcgccg tgtcgtcgtc gcagtcgggc gtggccaggc
     2641 tgaacacgac cacctcgtga ccggcgcgga ccaaggcccg agccaagccg tcgacgtggg
     2701 ccgcgatgcc gccgacgacc agcggcggat attcccacga cagcatcagc acgcgcacga
     2761 gatgggacgg tagcggctgg gcgcataggg tggcgtggtg atggcgagcg aacgtcccgt
     2821 ggccgattgg ccaggagagt tggacgtcca cctcttcggc gaggggcgtc acaggcggct
     2881 gtggcagttg ctcggcgcgc accggttgga acccgccggc ggcgccgaac gggggacccg
     2941 ctttgcggtc tgggccccca acgcggccca ggtgtgggtc gtcggcgact ggaatcagtg
     3001 gggcgacggc acggcgatgc acccggtggc cggaagcggc atgtggtgcg cgtcggtcgc
     3061 ggcggcccgt cccggccaca cctacaagta cgccgttgtc gacagcgcgg ggcgcaccgt
     3121 gctcaaggca nnnnnnnnnn cgatgccccg ccagaccgag tgtccgcccg gcccctccag
     3181 cgtggtgccc gccgagacgg cttacgtatg gcaggacgac gcgtggatcg agcgccgttc
     3241 gaaccgcccc agcgcgccgt tgcgcatcta cgaggtgcac ctgccgtcgt ggcgtcacgg
     3301 cgtcggcaac tacgtcgagc tcggtcacca gttggccgat cacgtcaccg cgttgggttt
     3361 cacccatgtc gagctgctgc cggtggccga gcatcccttc ggcgggtcgt ggggctacca
     3421 ggtcagcggc tactacgcgc cgacggcccg tctgggcacg cccgaccagt tccgcgagtt
     3481 cgtcgacatc ctccaccgtc gcggtgtcgg cgtgatcctc gactgggtgc cggcccactt
     3541 ccccaaggac gagtgggcgc tggcccgctt cgacggcacc gcnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnngttcc acatcgatgg tctgcgcgtc gacgccgtgg cgtcgatgct gtacctcgac
     3781 tacagccgcg agccggggca gtgggttccc aaccgcttcg gcggccgcga gaacctcggc
     3841 gcgatcgagc tgttgcgcga ggtcaacacc gtggtcggcg acgaggtgcc cacggcgatg
     3901 atgatcgccg aggagagcac gtcgtggcca aaggtgaccc acccggtgca cctgggcgga
     3961 ctggggttca gccacaagtg gaacatgggt tggatgcacg acacgctcgg ctacttcgaa
     4021 cgtgacacca tccaccgcca gtggcatcac aacgacctca ccttcggcct gctctacgcg
     4081 ttcggcgagc ggtacgtgtt gccgctgagc cacgacgagg tggtgcatgg caaggccagc
     4141 ctgctcggca agatgcccgg cgacgactgg cagcgcttcg ccaacctgcg cgccctgtac
     4201 gcgtggatgt gggcgttgcc gggcgcgccg ttgttgttca tgggcgccga gttggccccg
     4261 ttcagcgagt ggaacgaggc cgcggggttg ccgtggcatc tgctcgacca tgccccgcac
     4321 cgcggcgtgc gcgatctggt cgcgacgctc aacgcggtcg cggccgaagc gaccgagctg
     4381 tgggccggcg acaccgactc gtcgtcgttc cagtggctcg acgcggacga tgccctgcaa
     4441 ggcgcccgcc cccctgacac nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc gcttcggtgg cagcggcttt
     4621 cgcggcgagg gcatgacggt ggtggccgat gcggcgatcc cgtggcaggg ccaatccgcg
     4681 tcgctggtgg tcgatctgcc cccgttggcc gtgctgtggc tgcagtcgcc cgcgggctga
     4741 tcgctcggaa gatcgtgacg tgaacggtcg tgatgtgacc agccgtgatg tgaacggtcg
     4801 tgacgtgacc agccgtgatg tgaacggcgg g
//
LOCUS       DAORNF010000171         4796 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_13223_length_4796_cov_1.856359, whole genome shotgun sequence.
ACCESSION   DAORNF010000171 DAORNF010000000
VERSION     DAORNF010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4796
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13223_length_4796_cov_1.856359"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..690)
                     /locus_tag="PLV68_08055"
     CDS             complement(<1..690)
                     /locus_tag="PLV68_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01241.1"
                     /translation="MLDKIGTVTTVGGFIPAAGWVIAAGGAAFVALETYLNGTAGLYP
                     SSLSALETEINLPAFPEDFTIDGSWTTVTVTAQSTGWTADADIARTVLSTMSTATSGL
                     AGVKVADGLALDVSLYLRDNAVNGIVSETAQDGGLLTFCPQSWQVDVTGEPWSRGRAV
                     LSRFEVDSALRNYRPTETGLNLPVDDTLRVEVNPAAFAGESAWQDHTITVKPIRLSAP
                     AVVEIAEPGDEI"
     assembly_gap    724..754
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<755..1616)
                     /locus_tag="PLV68_08060"
     CDS             complement(<755..1616)
                     /locus_tag="PLV68_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01242.1"
                     /translation="MILFAPHHRVRRAVVALLAVALGACAGTTGSDLSAPPTTPAPTA
                     VATTALDEVGLSTGEVATLGRVELTQIHADDAGGLQLRLSTDDSDIGIPIFVDGATLY
                     FLAPMSPDDPMGGGQLELYLERDGTTGPMLRLDVEPMPDAPGAWLAFVDAFINEIDTA
                     ASGHGTTFAELQTIGFDAIDPSLIPLKLTQSYLDDGSSSDLESVWNAEQLTDEGRQLL
                     DAITAAMGLGSTFDSAEGLQSIMAPRSLSIGPAPLRSVSGSTTSATRSLRSAPIGGAC
                     VTAGISISGAD"
     gene            1712..>2593
                     /locus_tag="PLV68_08065"
     CDS             1712..>2593
                     /locus_tag="PLV68_08065"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRE01243.1"
                     /translation="MGAAGSDQRLRSGRSGSERSKRSSSVDRFVGRANEIAALTSALP
                     ATRPTGAVLWTVAGPAGIGKTTGPSARSSTDRQELAQVAVGSGHRVVWGQCWDEAGTP
                     PYWPWTQVIRQLRGNPIGVDLGSFVQPDAGPVDRFALFDATAVELRAAAAEVPLVVVL
                     DDLHHSDSLSLMLTRFVLAHLSDAPLLVVAAYRPNEAAARSDIIGHLAALRDDANELN
                     LSGLDLDGVRELVGDTLRADDVHTLTGGNPLFVEQIVRSAECPVAGTSSAPVASAGHA
                     ALLSAVTARLASVDAEVL"
     assembly_gap    2594..2607
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2608..3006)
                     /locus_tag="PLV68_08070"
     CDS             complement(<2608..3006)
                     /locus_tag="PLV68_08070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01244.1"
                     /translation="MIVDQAHGALVVGDRVGVGERLGGHLGRLAARRHGGLPVFPAGA
                     AEMEGALGRRRSTCDFEPCGGEVQRGQAIVGDQRHDRLGHQRMRQAPRFRSEQARVGD
                     TSGDTLQHVDVTTEQRCGRRAGGVGAEDRQG"
     gene            3273..>4796
                     /locus_tag="PLV68_08075"
     CDS             3273..>4796
                     /locus_tag="PLV68_08075"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HRE01245.1"
                     /translation="MANLAASHIAAAESARLADEAVRIGRRIGDAEALGIALVAQQVT
                     DLGPATLHRRLRTSREIIALAEMCGESDLAIRGRFLLKNALLEAGEVRELDAELLNQD
                     RLVNDTADVRFARHSLWFRCMRASFDGQAAQVESLARRCLEISQQLQDPDGFGVYAGQ
                     YGVALWMQGRLVELEPVYLDLMQAEPDAPLWPAVLGWIWLGERRIEAARGMLDRVPPP
                     EELPSGMHQLLSLYTTADLAVAVGDDTLAGEMWEALLPYADRVVPIAMGAACFGVIAR
                     PLGQLALRMGHVDEGIAHLERAVQITARIGARPWLVDAQLALAEALLDAGRADDRRLL
                     QLVLEASGTVASLDLAVFSDRVATIANRVESMRIATQVAPSIALHARAHSAENRALAR
                     VSVLGTFDVRAVDGSSPRWTSRKARSLLKILVARRGSPMAREQLMDLLWSDAHPDDVA
                     NRMSVAVSTVRRALDPGRTLPVDALVRADGGSLRLMFDAVEVDVESFLRHGQAALEAH
                     "
BASE COUNT          698 a         1588 c         1681 g          784 t
ORIGIN      
        1 gatctcgtcg ccgggctcgg cgatctcgac gaccgcgggg gccgagaggc ggatcggctt
       61 gacggtgatc gtgtggtcct gccacgccga ttcgccggcg aaggccgccg ggttgacttc
      121 gacgcgcaac gtgtcgtcga cgggcaggtt cagcccggtc tcggtggggc gatagttgcg
      181 caaggccgaa tcgacctcga agcgactcaa cacggcgcga ccgcgcgacc acggttcccc
      241 ggtcacgtcg acctgccagc tctgtggaca gaacgtcagc aggccgccgt cctgagcggt
      301 ttcgctcacg atcccgttga ccgcgttgtc gcgcaggtac agcgaaacgt cgagtgccag
      361 cccgtcggcg accttgacgc cggccagacc tgaggtggct gtcgacatcg tcgacagcac
      421 ggtgcgggcg atgtcggcgt cggcggtcca cccggtggac tgcgccgtca ccgtcaccgt
      481 cgtccatgag ccatcgatgg tgaagtcctc ggggaacgcc ggaaggttga tctcggtttc
      541 cagggcagac agcgaactcg ggtacagacc ggcggtgccg ttcaggtagg tctcgagcgc
      601 gacgaacgcg gcgccaccgg cggcgatcac ccagccggcg gcggggatga acccgcccac
      661 ggtcgtgacg gtgccgatct tgtcgagcac gtccctgcgg ggaccgtctg gcgagagcgt
      721 gttnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncgtcgg caccggatat ggagatgccc
      781 gccgtcacgc acgccccgcc gatcggagcg gagcggaggg accgcgtcgc tgacgtcgtc
      841 gagcccgata ccgagcgcaa cggagccgga ccgatcgata gggagcgcgg cgccatgatc
      901 gactggagac cttccgcgga atcgaacgtg gatccgaggc ccatcgcggc ggtgatggca
      961 tcgagcaact ggcgtccttc gtcggtcagt tgctcggcgt tccagacgct ctccaggtcg
     1021 ctgctggagc cgtcgtcgag gtagctctgg gtgagcttca acgggatcag cgacgggtcg
     1081 atggcgtcga atccgatcgt ctgcaactcg gcgaaggtcg tgccgtggcc gctcgctgcg
     1141 gtgtcgatct cgttgatgaa ggcgtcgacg aacgccagcc atgctcccgg tgcgtcgggc
     1201 atcggctcca cgtcgagccg caacatcggg ccggtcgttc cgtcgcgttc gaggtagagc
     1261 tcgagctggc cgccgcccat cgggtcatcg ggagacatcg gcgccaggaa gtacagcgtg
     1321 gctccgtcga cgaagatcgg gatcccgatg tcgctgtcgt cggtgctcag gcggagctgg
     1381 aggccgcctg cgtcgtccgc gtggatctgg gtgagttcca cccgtccgag ggttgccacc
     1441 tcgccagtcg acaggccgac ctcgtcgagc gcggtggtcg caacggccgt tggggctggt
     1501 gtggtcggcg gggcgctcag gtcggatcct gtcgtaccgg cgcaggcgcc gagcgcgacg
     1561 gcgagcaggg cgaccacggc gcggcgcact cgatggtggg gagcgaagag gatcatcagc
     1621 gcatccaggc tgtcaccgat cggtgaaagt ttgtcgtgat cagggccggc ccgttgccag
     1681 ggatgggcgg ggtgagcgat atgttcggcg gatgggtgcg gcgggatctg atcagcggct
     1741 ccgatctggc cggtcgggat cggaacgcag caagcgatcg agcagcgtcg accgcttcgt
     1801 cggaagggcc aacgagatcg ctgccctgac aagcgcactg ccggcgactc ggcctaccgg
     1861 cgccgtgctc tggaccgttg ccggacccgc cggcatcggc aagacgaccg gaccgagcgc
     1921 gagatccagc acggaccggc aggagttggc ccaggtcgct gtcggcagcg gacaccgagt
     1981 ggtgtggggg caatgctggg acgaggcggg caccccgccg tattggccgt ggacccaagt
     2041 gatacggcag ctccgaggga atcccatcgg tgtggacctc gggtcgttcg tccagcccga
     2101 tgctgggccg gtcgatcggt tcgcgctctt cgacgcgacg gccgtcgaac tccgtgccgc
     2161 agccgccgag gtcccgctgg tggtggtact cgacgatctg caccacagcg actcactgtc
     2221 gctgatgctg acccggttcg tgctggcaca tctctccgac gctcccctcc tggtcgtggc
     2281 ggcgtaccgg cccaacgagg ctgcagcccg ctccgacatc atcgggcatc tcgcggcact
     2341 gcgggacgat gccaacgagt tgaatctgag cggactcgat ctcgacggtg tccgcgaact
     2401 cgtcggagac acgctgcggg ccgacgacgt ccacacactg acgggcggca acccgctgtt
     2461 cgtggaacag atcgtccggt cggccgagtg ccccgtcgcc ggcacttcgt cggcccccgt
     2521 cgcatccgcg ggtcacgcgg cgttgctctc ggcggtgacg gcacggttgg cctcggtcga
     2581 cgccgaggtc ttgnnnnnnn nnnnnnngcc ttggcggtcc tcggccccca cgccaccggc
     2641 gcggcggccg cagcgctgct cggtcgtgac gtcgacgtgt tggagcgtgt cgccggacgt
     2701 gtcgccgacg cgggcttgct cggacctgaa ccgtggagcc tgtcgcatcc gctggtggcc
     2761 gaggcgatcg tggcgctggt cgccgacgat cgcctggccg cgttgcactt caccgccgca
     2821 gggctcaaag tcgcaggtgg atcgtcgcct gccgagcgcg ccttccatct ctgccgcgcc
     2881 ggcggggaac actgggaggc cgccgtggcg gcgtgctgcg aggcggccga ggtggcctcc
     2941 gagtcgttcg cccacgccga cgcggtcgcc cactacgagc gcgccgtggg cctgatcgac
     3001 gatcatgctg gaggccccga actggccttc gacgtgatgt tcgccatggc ggctgcgctc
     3061 gagcggtccg gtcggcgtct cgacgccgag gcaacctacg ggcgcgccta cgtccacgcc
     3121 ggcagatccg gcaacgccga gtcggtggct cgggccgctg cgcgccacgg gatctcgttc
     3181 tacgccgacg agcacctgca ccgtacgaga gccgatgaat gtcgcgcggc gttggagctc
     3241 tgccccggcg acagtgcatt gaggttcgtc tgttggccaa ccttgccgcg tcgcacatcg
     3301 ccgccgcgga gagcgcgcgt ctcgctgacg aggcggtccg catcggcaga cggatcggtg
     3361 atgccgaggc gctcgggatc gcgctcgtcg cccagcaggt caccgacctg ggaccggcaa
     3421 ccctgcaccg gcggctgcgc accagcaggg agatcatcgc cctggcagag atgtgcggcg
     3481 agtccgatct tgccatccgt ggccgcttcc tgctcaagaa cgcgctcctc gaagccggcg
     3541 aggtacgcga actcgatgcg gaactgctca atcaggaccg gctggtcaac gacactgcgg
     3601 atgtccggtt cgcgcgccac tcgttgtggt tccgatgcat gcgagccagc tttgacggcc
     3661 aagccgccca ggtcgagagc ttggccaggc gttgcctgga gatctcgcag cagcttcagg
     3721 acccggacgg cttcggcgtc tacgccggcc agtacggcgt cgcgctctgg atgcagggcc
     3781 gtctcgtgga actcgagccg gtctacctcg acctgatgca ggccgaacct gatgccccgc
     3841 tgtggcctgc cgtgctcggc tggatatggc tcggtgagcg acgcatcgag gcagcccgcg
     3901 gaatgctcga tcgagttccc ccgcccgagg agcttccgag cggcatgcac cagctgctca
     3961 gtctctacac cacagccgac ctcgccgtcg cggtcggcga tgacacgctc gcgggcgaga
     4021 tgtgggaagc cttgctgccc tacgccgacc gagtcgtgcc gatcgccatg ggcgccgcgt
     4081 gcttcggggt gattgcacgg ccgttgggtc agctggcact gcggatgggg cacgtcgacg
     4141 agggcatcgc ccacctcgag cgggccgtgc agatcactgc acggattggc gcccgcccgt
     4201 ggttggtcga cgcacaactg gcgctggccg aagcccttct cgatgccggc cgagccgacg
     4261 accgacggct cttgcagctc gttctcgagg cgtccggcac cgtcgcgagc ttggacctcg
     4321 cggtgttctc ggatcgcgtc gccaccatcg ccaaccgggt tgaatcgatg cgaatcgcga
     4381 cacaagtggc gccctccatc gcgttgcacg cacgggctca ctcggccgag aatcgcgcgc
     4441 tggcgagggt gtctgtgctc ggcacgttcg acgtacgggc cgtcgacggg tcgtcgcctc
     4501 ggtggacgtc gcgcaaagca cggtcactcc tcaagattct cgtggcacgc cgaggctcac
     4561 cgatggcccg cgagcagctg atggacctgc tctggtccga cgctcatccc gacgacgtgg
     4621 ccaatcggat gtcggtcgcc gtctccacgg tgcgccgcgc gctcgatcct ggccgcacgt
     4681 tgccggtgga cgcgctggtc cgcgcagatg gcggcagtct ccgcctcatg ttcgatgccg
     4741 tcgaggttga cgtcgagtcg ttcctacggc acggccaagc cgccctcgag gcccac
//
LOCUS       DAORNF010000172         4794 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_13229_length_4794_cov_1.948512, whole genome shotgun sequence.
ACCESSION   DAORNF010000172 DAORNF010000000
VERSION     DAORNF010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4794
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13229_length_4794_cov_1.948512"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="PLV68_08080"
     CDS             <1..522
                     /locus_tag="PLV68_08080"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRE01246.1"
                     /translation="DALRAGVESLVVGDPADEATDVSALISPSERDRVCSWVDEAVAG
                     GARVVTGGTVGANGVLAPTVLADVIPSMKVCAGEVFGPVVAIATYTDLDTALRDANDS
                     AYGLQAAIFTSNIAVALRAIRTLDYGGVLVNEVPTWRADQQPYGGLRDSGNTREGPAY
                     SVKEMTEIRMVVL"
     gene            complement(626..>1798)
                     /locus_tag="PLV68_08085"
     CDS             complement(626..>1798)
                     /locus_tag="PLV68_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRE01247.1"
                     /translation="DRTIPGYESYEAAVAAQPAEPLAERIAGADMLYSSGTTGMPKGV
                     TGKFEARDLTQSAVGVSALLSMLFGGSDQSVYLSPAPLYHAAPLRFNMSIHALGGTTV
                     IMEHFDPEQYLALIERYKITISQVVPTMFVRLMKLPDEVQHRYDVSSIQTIIHAAAPC
                     PIPVKKAIIEWFGPVVHEYYAGTEGNGFVYCNSEMWLAHEGTVGMPIACTLHIVGDDG
                     SELPPYESGTIYFEGGAEFEYHNDPDKTASSRHPKGWSTLGDVGYLDNDGFLYLTDRK
                     AYMIISGGVNIYPQEAENILVTHPKVIDCAVFGVPNDDFGEEVKAVVQPVEMPADDAA
                     AAELASELIRFCRSHLADVKCPRSIDFRPELPRHPTGKLYKRLLKDEYWREAGRNI"
     assembly_gap    1799..1808
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1809..2173)
                     /locus_tag="PLV68_08090"
     CDS             complement(<1809..2173)
                     /locus_tag="PLV68_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRE01248.1"
                     /translation="MFPGAHAQTTPDKPAIIMAATGQVTTFAELDAAANRLSQLLYAA
                     GLRPGDHIAVCMENHPRYLEVIWGCHYAGLVYTACSSRLTTDELVYILNDCGARVFIT
                     SAYKAEQAAEIISRAPNIEL"
     gene            2207..2917
                     /locus_tag="PLV68_08095"
     CDS             2207..2917
                     /locus_tag="PLV68_08095"
                     /inference="COORDINATES: protein motif:HMM:NF024762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HRE01249.1"
                     /translation="MEPSVAKQRLRPLLRSSEGSLPLGEVCLLISAVAAGTAPQVFPA
                     VNVEAGLARLDALAADAPSSFAALMPHLFTGRHGWMANRQHYYAVSNSLLSEVLRTRQ
                     GIPISLSIVAIEIGRRCGSDVVGIGMPGHFLVHSIDDGDLFADPFDRGQLMNREDARA
                     LFGRLTHGTGRWHDSYLRPVSDLQIVQRTLNNLAVAARKQPFADIHLPWVLEVMSWLP
                     GGAPFDPREASRAVAIHN"
     gene            2996..4201
                     /locus_tag="PLV68_08100"
     CDS             2996..4201
                     /locus_tag="PLV68_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRE01250.1"
                     /translation="MTDTAIDVTADEQRVIDLTEALLASYPPQSTDPVTFLGAQFDSG
                     LAWVHFPEGHGGLGLNPKLQKTINERVFAAGAPNPTARNPIGHGMCGPTVAVWGSEAQ
                     KSRYLRPLFTGEEVWCQLFSEPGAGSDFAGLSARGIKDGDEWVCNGQKVWTTLGHLAR
                     WGLLVVRTDPEAVKHAGLTAFVVDMHAPTVQVLPLRQMTGEAEFNEVFFTDTRIPNSE
                     MLGQPGDGWRVSLTTLMNERVSIGGSIPTKGSGPVRELMKVWNGMPADAKTPALRDEV
                     TQLWIRAELLRLTNIRANQRRKMGDPGPEGSIGKGASAELNKDLYNFIMNLLGAEGML
                     YGSYEMVQPDFAMGFDSLQKAFLRMRANSIEGGTTEVMKNILGERVLGLPGDVRVDRD
                     RPWSEVPRN"
     assembly_gap    4454..4492
                     /estimated_length=39
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4493..4697)
                     /locus_tag="PLV68_08105"
     CDS             complement(<4493..4697)
                     /locus_tag="PLV68_08105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01251.1"
                     /translation="MLSPTAATIRLTLHVLAACIWVGGQFTLAALVPAVRGHDGIAKI
                     AANAFSRIAWPAFGVLVLTGVWNL"
BASE COUNT          762 a         1516 c         1658 g          809 t
ORIGIN      
        1 gacgcgctgc gtgcgggggt ggagtcgttg gtggtcggcg acccggccga cgaggcgacc
       61 gacgtgtcgg cgctgatctc acccagcgag cgcgaccggg tgtgttcgtg ggttgacgag
      121 gctgtggccg ggggagcgcg ggtcgtcacc ggcggcaccg tcggcgcgaa cggcgtgctc
      181 gccccgacgg tgctggccga cgtgatcccc tcgatgaagg tctgcgcggg cgaggtgttc
      241 ggccctgtgg tggcgatcgc cacctatacc gatctcgaca ccgcgctgcg cgacgccaac
      301 gacagcgcct acggcctcca ggcggcgatc ttcaccagca acatcgccgt cgcgctgcgg
      361 gccatccgca cgctcgacta cggcggcgtg ttggtcaacg aggtgcccac gtggcgcgct
      421 gaccagcagc cctacggcgg cctgcgcgac agcggcaaca cccgcgaagg cccggcctac
      481 agtgtcaagg agatgaccga gatccggatg gtggtgctgt agctgctgtg gcctgtcggc
      541 gtgccgctcc gtcccgcgta cgccgacagg tcacactccc gcacactatt ccatccgtca
      601 ccctgccgtt gacgcgtcac cgtgttcaga tgttgcggcc cgcctcacgc cagtactcgt
      661 ctttcagcaa tctcttatag agttttccgg ttgggtgtcg ggggagctcg gggcggaagt
      721 cgatgctgcg cgggcacttc acatcggcca agtggctgcg gcagaagcgg atcagttcgc
      781 ttgccaactc ggcggcagcg gcatcgtccg cgggcatctc caccggttgg accacggcct
      841 tgacctcttc accgaaatcg tcgttgggca cgccgaagac ggcgcagtcg atgaccttgg
      901 ggtgggtgac caggatgttc tcggcctctt ggggatagat gttgaccccg ccggagatga
      961 tcatgtacgc cttgcggtcg gtcaggtaca ggaagccgtc gttgtcgagg tagcccacgt
     1021 cgccgagcgt gctccatccc ttcgggtgac gtgaactggc ggtcttgtcg gggtcgttgt
     1081 gatactcgaa ctcggcgccg ccttcgaagt agatcgtgcc gctctcgtac ggcggcaact
     1141 cggaaccgtc gtcgccgacg atgtgcaggg tgcaggcgat cggcatgccg acggtgcctt
     1201 cgtgggccag ccacatctcg ctgttgcagt acacgaagcc gttgccttcg gtgccggcgt
     1261 agtactcgtg caccaccggg ccgaaccact cgatgatcgc cttcttgacc gggatcgggc
     1321 agggggcggc ggcatggatg atggtctgga tcgagctgac gtcgtaccgg tgttgcacct
     1381 cgtcgggcag cttcatcagc cggacgaaca tcgtcggcac cacctggctg atcgtgatct
     1441 tgtagcgctc gatcagggcc agatactgct cgggatcgaa gtgctccatg atcacggtgg
     1501 taccaccgag ggcgtggatc gacatgttga agcgcagcgg cgcagcgtga tacagcggtg
     1561 cgggtgagag gtagaccgac tggtcggaac caccgaagag catgctcagc agggcgctga
     1621 caccgacggc cgactgggtg agatcgcgcg cttcgaactt gccggtgacg cccttgggca
     1681 taccggtggt gcccgacgag tagagcatgt cggcaccggc gatgcgctcg gccaagggct
     1741 cggccggctg tgcagccacc gcggcttcat agctctcgta gcccgggatg gtgcggtcnn
     1801 nnnnnnnnag ctcgatgttg ggtgcgcgcg agatgatctc ggcagcttgc tcggccttgt
     1861 aggcactggt gatgaacacg cgcgcgccgc agtcgttgag gatgtagacc aactcgtcgg
     1921 tggtcagacg gctggagcag gcggtgtaca ccaacccggc gtagtgacat ccccatatca
     1981 cctccagata gcgagggtgg ttctccatgc acaccgcgat gtggtcgcca gggcgcaacc
     2041 ccgcggcgta caacaactgg ctcagccggt tggctgcggc atcgagttcg gcgaaggtcg
     2101 tgacctggcc ggtcgcggcc atgatgatgg ctggcttgtc aggtgtcgtc tgcgcatgcg
     2161 ctcccggaaa catgggccgc acggtagtcg ccgctacggt cgtggtgtgg agccgagcgt
     2221 cgccaagcaa cgactgcgcc cgctgctgcg gagttcggag gggtcgctgc cgctcggtga
     2281 ggtatgcctg ctgatatcgg cagtcgccgc ggggacggcg ccgcaggtgt ttcccgcggt
     2341 gaacgtcgag gccggtctgg cccgcctcga tgcgctcgct gcggacgcac ccagttcgtt
     2401 cgcagcgttg atgccgcacc tgttcaccgg ccgccacggc tggatggcca accgccagca
     2461 ctactacgcc gtgtccaact cgctgctgag cgaagtgttg cggacgcgtc agggcattcc
     2521 gatcagcctc tccatcgtgg cgatcgagat cgggcggcgt tgcggctcag acgtcgtcgg
     2581 catcggtatg ccgggacact tcctggtgca ctcgatcgac gacggtgatc tgttcgccga
     2641 ccccttcgat cggggccagc tgatgaaccg cgaggatgcc cgcgccctgt tcggccggct
     2701 cacccacggc accggacgct ggcacgactc gtacctgcgg ccggtgtccg acctgcagat
     2761 cgtccagcgc accctcaaca atctggccgt ggcggcgcgc aagcaaccgt ttgccgacat
     2821 ccacctgcca tgggtgctcg aagtgatgag ctggctgccc ggtggcgcgc cgttcgaccc
     2881 ccgcgaggcc agccgcgccg tggccattca caactgagcc gggtggttcg gctcgcgggg
     2941 cgatgtgccg ggtcggtaac gtcgcggggt ccgctcttgt acaccggaga atccgatgac
     3001 cgataccgca atcgacgtga ccgctgacga gcagcgcgtc atcgacctga ccgaggctct
     3061 gctcgcttca tacccaccgc agtcgaccga ccccgtgacg ttcctgggtg cacagttcga
     3121 ctccggcctg gcgtgggtgc actttccgga aggccacggt ggcctgggtc tcaaccccaa
     3181 gctgcagaag acgatcaacg agcgcgtctt cgctgccgga gcacccaatc ccaccgcgcg
     3241 caacccgatc ggccacggca tgtgcggccc gacggtggcg gtgtggggca gcgaagccca
     3301 gaagtcgcgc tatctgcggc ccttgttcac cggtgaagag gtctggtgcc agctgttcag
     3361 cgaacccggc gccggcagcg atttcgccgg cctgtcggct cgcggcatca aggacggcga
     3421 cgagtgggtg tgcaacggcc agaaggtgtg gaccacgctc ggccatctcg cccgctgggg
     3481 tctgctggtg gtacgcaccg accccgaagc ggtcaagcac gcaggcctca ccgcgttcgt
     3541 cgtcgacatg cacgcgccga ccgtgcaggt gttgccgttg cgccagatga ccggcgaagc
     3601 ggagttcaac gaggtgttct tcaccgacac ccgcattccc aacagcgaga tgttgggcca
     3661 acccggcgac ggatggcgcg tgtcgctgac cacgctgatg aacgagcgcg tctccatcgg
     3721 cggcagcatc ccgaccaagg gcagcgggcc ggtgcgcgag ctgatgaagg tgtggaacgg
     3781 catgcccgcc gacgccaaga ccccggcgtt gcgcgacgag gtcacccagt tgtggatccg
     3841 ggccgagctg ctgcggctca ccaacatccg cgccaaccag cggcgcaaga tgggcgatcc
     3901 cggtcccgag ggctcgatcg gcaagggtgc ctcggccgag ctcaacaagg acctctacaa
     3961 cttcattatg aacctgctcg gtgccgaggg catgctctac ggcagctacg agatggtgca
     4021 gcccgacttc gcgatgggct tcgattcgct gcagaaggcg ttcttgcgca tgcgcgccaa
     4081 ctcgatcgag ggtggcacca ccgaggtgat gaagaacatc ttgggcgagc gcgtgttggg
     4141 cctccccggc gacgtccgcg tcgaccgcga tcgcccctgg agcgaagtcc cccgcaactg
     4201 acccgtcacc ccgccgagcc agcccgcgcc cgcggtgagt ctgccgattt caccgggccg
     4261 cggtacgggt tggctcgtca ggtgtaggtg ccggttcgga ggaggacgcc gacgaagacg
     4321 gcagcgaggg cggtgaggcc accgatggcg ccgcccaacg cgaggccgag tttggtggtg
     4381 gcccgggtgt gggcggcggc ggcgatgccc gacacggcgg cgacgatgat cttgacgaac
     4441 acggtgacct gatnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnccaggttc
     4501 cacacgccgg tcagcaccag taccccgaac gcgggccagg cgatgcggct gaacgcgttg
     4561 gcggcgatct tggcgatgcc atcgtggccg cgcacggcgg gtaccagagc ggccagcgtg
     4621 aactggccgc cgacccagat gcaggcggcg agcacgtgga gggtgaggcg gatcgtggcg
     4681 gcggtggggg acagcatggt gcgcagttcg ttctcgtgaa gggggatgga cggggaatcg
     4741 ggatcaggtg gcgagcgccg ccagcacgtc gctggcgggc acggcgcggt cgtc
//
LOCUS       DAORNF010000173         4776 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_13274_length_4776_cov_1.484008, whole genome shotgun sequence.
ACCESSION   DAORNF010000173 DAORNF010000000
VERSION     DAORNF010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4776
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13274_length_4776_cov_1.484008"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>257)
                     /locus_tag="PLV68_08110"
     CDS             complement(<1..>257)
                     /locus_tag="PLV68_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01252.1"
                     /translation="EAIALGDQIVASSWWQTHVGARPRLVAARADANSSRYVSARIGS
                     APGTVRFMHTVPRFVVVHELAHAAVHIASGTADHGHDALWR"
     assembly_gap    258..475
                     /estimated_length=218
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<476..1211)
                     /locus_tag="PLV68_08115"
     CDS             complement(<476..1211)
                     /locus_tag="PLV68_08115"
                     /inference="COORDINATES: protein motif:HMM:NF022432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HRE01253.1"
                     /translation="MHQLSFDTSTDDTSRAAAHDPSSSSGDGAAAIDMLVSRIEKLWR
                     KANDSAATPAERELFETKALALMQRHRIDAEMLDVEHGDPIRELSYGRVDSRYAGVVC
                     GLIHCIAASYGCKVWWTQHADHRDVVVFGFRRDTERVVHLGRMLVADAMAQASQLSGP
                     TGGQSFARRRSFVMGYAEGVTIRLQAAAAMAREQAEAEHGERGRSAALVVVSRPQQVD
                     GAFSARWKGKGMRTVSVAAASSEHAYR"
     gene            1333..1758
                     /locus_tag="PLV68_08120"
     CDS             1333..1758
                     /locus_tag="PLV68_08120"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HRE01254.1"
                     /translation="MASKRASLDHLRNVPLFAACTTRDLQRIAKAGDEIKMPAGSLIV
                     DQGQTGREAYVVLSGTVTVRRNGKKVASLGPGAIVGELSLLDHGPRTASVYAETDIEL
                     LVITQRHFTAVLDEVPALSHRLMQALAGRIRELDRSYYG"
     gene            1866..>2261
                     /locus_tag="PLV68_08125"
     CDS             1866..>2261
                     /locus_tag="PLV68_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur protein"
                     /protein_id="HRE01255.1"
                     /translation="MSTHSSAPAPDKSPKRFKPYQLVLTMGIFMAVFTVVAGILPLIT
                     KWHSDSDVTRHTFSGIPGPLQIAFYTVIPALIVWGAFAFADRVRNWERGGPDRRRTTV
                     KNAKKRLGDFRAGVYMRTLLRDSGAGLMHS"
     assembly_gap    2262..2342
                     /estimated_length=81
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2343..4153
                     /locus_tag="PLV68_08130"
     CDS             <2343..4153
                     /locus_tag="PLV68_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HRE01256.1"
                     /translation="KFLHGRTYMAYSFIGDLAGLIFTGGIIWAIVRRYIQRPYRIRIK
                     TKPEHAVILGVFLAIGLSGLWAEGVRIAEQGQPSFEKWSFIGYPISHLYSGMSASALS
                     TWHQVAWSIHVVSFIAFLAILPITMLRHMFTSPLNMYLKDRERPKGAMRAMPNLTETE
                     LETFGASVVEDFTWKQLLDTDACTMCGRCTSVCPAHATGKPLDPREIVLKVGEVMAAT
                     SVDPTFGHVSPPIGTVAEISITSNSVFERITAEEIWSCTSCRACDEICPVNIEILDKI
                     LDMRRYLSLMESNFPAELGNAYRAMENQGNPWGMSQAERGDWADGLDVTVVDPGETFS
                     AEYLYWVGCAGSFDDKNKKVTQSMAKLLKRAGIDVAILGPSEMCTGDSARRSGNEYLF
                     QMLAMPNIEMLNGMGVKKIITQCPHCFNTLKNEYPQLDGNYEVVHHTQLLESLIESGA
                     LDVSDATLEERITYHDSCYLGRHNDVYLAPRKVVASIKGIEVVEMQRNGTKGMCCGAG
                     GARMWMEENVGTKVNDERAVEAISTGASRVATACPFCYIMLDDGVKAAGKEDSDIKVA
                     DIAIHLLEAIENGERQRLDNMAAAPLRRVETPVAGD"
     assembly_gap    4482..4567
                     /estimated_length=86
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4568..>4776
                     /locus_tag="PLV68_08135"
     CDS             <4568..>4776
                     /locus_tag="PLV68_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01257.1"
                     /translation="APTMSVINYRPGQTRSNTVAANVSASGRIGLRSTGSSDLVVDIM
                     GYFAPTTSASAGRVVAVTPRRLLDTR"
BASE COUNT          760 a         1516 c         1435 g          680 t
ORIGIN      
        1 tcgccacagc gcgtcgtggc cgtggtcggc ggtgccgctg gcgatgtgca ccgcggcgtg
       61 ggccagttcg tggacgacca cgaagcgggg gacggtgtgc atgaaccgca ccgttccggg
      121 tgccgagccg atgcgggccg agacgtagcg cgacgagttc gcatcggcgc gggccgcgac
      181 cagacgcggt cgcgcgccga cgtgcgtttg ccaccacgac gacgcgacga tctggtcgcc
      241 gagcgcgatc gcctccgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncccgg
      481 taggcgtgct cgctgctggc cgcggcgacc gacaccgttc gcatcccctt gcccttccag
      541 cgagccgaga acgcaccatc gacctgttgc gggcgggaca ccacaaccaa ggccgccgac
      601 ctgccgcgct cgccatgctc tgcctcggcc tgctcgcggg ccatggccgc ggcggcctgg
      661 aggcggatgg tgacaccctc tgcataaccc atcacgaacg aacgtcgcct ggcgaaggac
      721 tggccaccag tcggcccgga aagctgcgac gcctgggcca tcgcgtcggc caccagcatc
      781 cggccgaggt gcacgacccg ctcggtgtcg cggcgaaaac cgaacacgac gacatcgcga
      841 tggtccgcgt gctgcgtcca ccacaccttg cacccgtagc tcgcagcgat gcagtggatc
      901 aggccgcaga cgacccccgc gtagcgggag tcgacccgcc cgtagctcag ctcgcggatg
      961 gggtcgccgt gctcgacatc gagcatctcg gcatcgatcc ggtggcgttg catcaacgcc
     1021 agggccttgg tctcgaacag ctcccgctcg gcgggggtgg cagcgctgtc gttggccttg
     1081 cgccacaact tctcgatccg gctgacgagc atgtcgatcg cggcagcacc gtcaccggat
     1141 gacgacgacg ggtcatgcgc ggcggcgcgt gacgtgtcgt cggtggatgt gtcgaagctg
     1201 agctggtgca tggcgaaccc ccgtggacgg tagcggttgg cggggtgctg gcgacccgat
     1261 cgaacacccg tgatgatgca ccaggggtgt gatgcgattt cgtggctggc ggaccagggt
     1321 aatgtgcggc ccatggccag caagagggca tcactcgacc acctgcgcaa cgttccgctg
     1381 ttcgcagcct gcaccaccag agacctgcag aggatcgcca aagcgggcga cgagatcaag
     1441 atgcctgccg gatcgctcat cgtcgatcag ggccagaccg ggcgtgaggc ctacgtcgtg
     1501 ctcagtggca cggtcaccgt gcgccgcaac ggcaagaagg tcgcgtcgct cggccccggc
     1561 gcgatcgtcg gcgaactgtc gctgctcgac cacggcccgc gcaccgcgtc ggtgtacgcc
     1621 gagaccgaca tcgagttgct ggtcatcacc cagcgccact tcacggccgt gctcgacgag
     1681 gtgccagcgc tgtcacaccg cttgatgcag gcgctcgctg gccgcattcg cgaactcgac
     1741 cgcagctact acggctgagc tgttccggct gatcgggcag ttgctgcgga tggccgctca
     1801 cagcggtcgc ccgccaagcg ccactaggcg ttccttcgcg cccgcgaact agagtttcgg
     1861 gcgtcatgag cacccacagt tctgcaccag ctccggacaa gtccccgaag cgcttcaagc
     1921 cctatcagct cgtactgacg atgggcatct tcatggccgt tttcacggtc gtggcgggca
     1981 tcttgccgtt gatcaccaag tggcacagcg acagcgacgt cacccgccac acgttctcag
     2041 gcatacccgg cccgctccag atcgcgttct acaccgtgat cccggcactg atcgtgtggg
     2101 gagcattcgc gttcgcggac cgggtgcgca actgggagcg gggcggtccc gaccggcgcc
     2161 gcaccaccgt caagaacgcc aagaagcgcc tgggtgactt ccgcgccggg gtgtacatgc
     2221 gcacgctgct gcgcgactcg ggcgccgggc tgatgcacag cnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nntcaagttc ctgcacgggc gcacctacat ggcctactcg ttcatcggtg acctggccgg
     2401 gttgatcttc accggcggca tcatctgggc catcgtgcgc cgctacatcc agcgcccgta
     2461 tcgcatccgt atcaagacca agcccgagca cgcggtgatc ctcggcgtgt tcctcgccat
     2521 cggcctcagc ggcctgtggg ccgaaggggt gcgcatcgcc gagcagggtc agcccagctt
     2581 cgagaagtgg agcttcatcg gctacccgat cagccacctg tactcgggta tgtccgcctc
     2641 ggcgctcagc acgtggcatc aggtggcgtg gagtatccac gtcgtcagct tcatcgcctt
     2701 tcttgcgatc ctgcccatca ccatgctgcg gcacatgttc acctcgccgc tcaacatgta
     2761 cctcaaggat cgcgagcggc ccaagggcgc gatgcgagcg atgcccaacc tcaccgagac
     2821 cgagctcgag accttcggtg cgtccgtcgt cgaagatttc acctggaagc agttgctcga
     2881 caccgacgcc tgcaccatgt gcggacgctg caccagcgtg tgcccggcgc atgccaccgg
     2941 caagccgctc gacccgcgcg agatcgtgct caaggtcggc gaggtcatgg cggccacgtc
     3001 ggtcgacccc accttcggcc acgtgtcgcc gccgatcggc accgtcgcgg agatctccat
     3061 cacctccaac tcggtgttcg agcggatcac cgccgaggag atctggtcgt gcaccagctg
     3121 ccgggcatgc gacgagatct gcccggtcaa catcgagatc ctcgacaaga tcctcgacat
     3181 gcgccggtac ctgtcgctga tggagagcaa cttcccggcc gagctcggca acgcctaccg
     3241 tgccatggag aaccagggca acccgtgggg catgagccaa gccgaacgcg gcgattgggc
     3301 cgacgggctc gacgtcaccg tggtcgatcc gggagagacg ttcagcgccg agtacctcta
     3361 ctgggtcggg tgcgccgggt cgttcgacga caagaacaag aaggtcaccc agtcgatggc
     3421 caagctgctc aagcgggccg gcatcgacgt ggcgatcctc ggcccctccg agatgtgcac
     3481 cggcgactcg gcccggcgca gcggtaacga gtacctgttc cagatgctgg cgatgccgaa
     3541 catcgagatg ctcaacggca tgggcgtcaa gaagatcatc acccagtgcc cgcactgctt
     3601 caacacgttg aagaacgagt acccgcaact cgacggcaac tacgaggtgg tgcaccacac
     3661 ccaactgctc gaatcgctga tcgagagcgg tgcgctcgac gtcagcgacg ccacgctcga
     3721 agagcggatc acctaccacg acagctgcta cctcggccgc cacaacgacg tctacctcgc
     3781 cccgcgcaag gtggtcgcca gcatcaaggg catcgaggtg gtcgagatgc agcgcaacgg
     3841 caccaagggc atgtgctgcg gtgccggtgg ggcgcggatg tggatggaag agaacgtcgg
     3901 caccaaggtc aacgacgagc gagcggtcga ggcgatctcc accggcgcca gccgcgtggc
     3961 caccgcctgc ccgttctgct acatcatgct cgacgacggt gtgaaggctg cgggcaagga
     4021 agactccgac atcaaagtgg ccgacatcgc catccacctg ctcgaagcga tcgagaacgg
     4081 cgagcgccag cgactcgaca acatggccgc agccccgctg cgccgtgtcg agactccagt
     4141 cgcaggcgac tgagcgcaca acccccctcg gcacacccac ctgcccgcga ctagcgtggg
     4201 ccgtgggata tggcgggatg cggacacacg gcgcgacacc agggccggat cgcgcgcggt
     4261 atgtggtcgg tcgccgtgtg cgccaccgtg gccgtggtgg catccatcgg gtcgatcgca
     4321 gggccaatgg tcgtggcacc gcccgtgcac gcgagcggac ccggtgagag cccgctggcc
     4381 gcggcgttcc acccgttggc gccgtgtcgc atcaccgaca cgcgcgagtc cgcgcccggc
     4441 cggcgagacc gcgatgtgat cgtcgacgtc gccggtcact gnnnnnnnnn nnnnnnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnngcg ccgaccatgt cggtgatcaa ctaccggccc ggtcagacgc gcagcaacac
     4621 ggtcgctgcg aacgtgtcgg cctcgggccg catcggcctg cgctcgaccg gcagcagcga
     4681 tctggtggtc gacatcatgg gctacttcgc gccgacgacg agcgccagcg cgggccgcgt
     4741 cgtcgccgtc acgccgcggc ggctgctcga cacgcg
//
LOCUS       DAORNF010000174         4774 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_13283_length_4774_cov_1.584658, whole genome shotgun sequence.
ACCESSION   DAORNF010000174 DAORNF010000000
VERSION     DAORNF010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4774
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13283_length_4774_cov_1.584658"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>230)
                     /locus_tag="PLV68_08140"
     CDS             complement(<1..>230)
                     /locus_tag="PLV68_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HRE01258.1"
                     /translation="TPALAARYAAEFNTPFLSPDAFGAQRARVIDACAAIGRDRPMAF
                     SATLAVCVGRDEAEITRRAAAIRREPSELRQN"
     assembly_gap    231..322
                     /estimated_length=92
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<323..780)
                     /locus_tag="PLV68_08145"
     CDS             complement(<323..780)
                     /locus_tag="PLV68_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HRE01259.1"
                     /translation="MQLADLRIFTEPQQGATYDTLLAVARRAEALGFGAFFRSDHYVV
                     MGDFDGLPGPTDAWITLAGLARDTTTIRLGTLVSPVTFRSPGVLAISVAQVDQMSGGR
                     AELGLGAGWFQREHDAYALDFPALKDRFTSLADQLAIIDGLWSAPIGETFT"
     gene            complement(835..1470)
                     /locus_tag="PLV68_08150"
     CDS             complement(835..1470)
                     /locus_tag="PLV68_08150"
                     /inference="COORDINATES: protein motif:HMM:NF021378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HRE01260.1"
                     /translation="MTVLIIFVVFRSVAKAGGGMRAALGMGPSADKQQEIARLQATGT
                     KARAHILGAQPTGLVVNNINIGIDLHVMLEPLDGSPRFQASKRMVVSQVNLPRVGDVW
                     PAWFDPADHTKFALAMVTQFTPEVAALFAQFGIANPLGGATAFPGGMAGAAPAGYPGA
                     PQQAGFAAPAMGMPSPGAMNPAVELERLATLHAQGALSSAEFEQAKRRLLG"
     gene            1853..>2219
                     /locus_tag="PLV68_08155"
     CDS             1853..>2219
                     /locus_tag="PLV68_08155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01261.1"
                     /translation="MVGGESYVVAGVVSLAVLHDGVDAVAEQIAGAVERGGLLGLAVS
                     PAGVGLAVAASTAWAVETDDPLALVVAIERRVRPRWAWWNAATASWLLAEGVRIDLCW
                     DIAAVQRMLYGGWRADPARV"
     assembly_gap    2220..2333
                     /estimated_length=114
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2334..>2627
                     /locus_tag="PLV68_08160"
     CDS             <2334..>2627
                     /locus_tag="PLV68_08160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01262.1"
                     /translation="DAEQPVQPDGHLRPEWALDDGTGQGWSVSAQRLARWAAAAMAAV
                     ERQGAAIAAHPARARLERTVRSESSAELLGAEMAHGGLPFDLAAAEHLIGSLVG"
     assembly_gap    2628..2752
                     /estimated_length=125
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2753..3686
                     /locus_tag="PLV68_08165"
     CDS             <2753..3686
                     /locus_tag="PLV68_08165"
                     /inference="COORDINATES: protein motif:HMM:NF012688.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HRE01263.1"
                     /translation="IDVPDTRAWRLKPLRGLHPVVDALLTWRKAERIETTFGYGWIDQ
                     HVRGGRLRGEWSGSDGAAGRMTASAGLHNMPADLRIAVAADPGFTFVRADLGQIEPRV
                     LAAVSGDPALTAAAEQADLYAPVAARLGVERAVAKVAVLAAMYGQTSGTAGMALQRME
                     TEYPVALQYLRDADSAGRAGQDITTYGGRLVRMWDFGDQYAEQPQRERAAAAARGRFA
                     RNAIVQGAAAELFKAWAVIVRARVAPLGAAVVLCLHDEVLVHTPAEHAAEVAELIERC
                     LQEAAWRHFDDGPARRVRFVADVSIIARWSDAKE"
     gene            3824..>4399
                     /locus_tag="PLV68_08170"
     CDS             3824..>4399
                     /locus_tag="PLV68_08170"
                     /inference="COORDINATES: protein motif:HMM:NF012242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HRE01264.1"
                     /translation="MAHWTLAIDFGTSFTVAAVRVEGRAPEIVEIAGERRVPSVVLVD
                     PSGSVFVGRTAEDLSVTHPGNTLRALKNRLGDATPVIVGGRPHQVVTLVAALLAEVYS
                     AVVTQMGEPPSEVRLTHPATWNQPRLNRLIEAAAKAGMPEPLLVPEPVAAALSYAADV
                     GVSAGRPIAVYDLGGGTFDSAVVVARESGCEV"
     assembly_gap    4400..4498
                     /estimated_length=99
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4499..>4774
                     /locus_tag="PLV68_08175"
     CDS             <4499..>4774
                     /locus_tag="PLV68_08175"
                     /inference="COORDINATES: protein motif:HMM:NF012242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HRE01265.1"
                     /translation="DQIQVGDDALWRQVGAALRNEVRKAKEALSSHPYATVLVPLPTG
                     LTQFRLTRDEFAGLIETYLDETISMMRRSLQEAGVAPAELAGISLVG"
BASE COUNT          618 a         1421 c         1634 g          671 t
ORIGIN      
        1 gttctggcgg agctcggatg gctcgcggcg gatcgccgcg gcgcggcggg tgatctcggc
       61 ctcgtcgcgc ccgacgcaga ccgccagcgt cgccgagaac gccatcggtc ggtcgcggcc
      121 gatggccgcg caggcgtcga tgacgcgcgc ccgttgcgcg ccgaacgcgt cgggagacag
      181 gaacggcgtg ttgaactcgg ccgcgtagcg agcggccagg gcaggcgtct nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nngtgaaagt ctcgccgatc ggcgccgacc acagcccgtc
      361 gatgatggcg agctggtcgg ccagactggt gaagcgatcc ttcaacgcgg ggaagtcgag
      421 cgcgtaggca tcgtgttcgc gctggaacca accggcgccc aggccgagct cggctcgtcc
      481 accgctcatc tggtcgacct gggcgaccga gatcgcgagc acgccggggc tgcggaacgt
      541 cacggggctg accaaggtgc cgaggcggat cgtggtggtg tcacgggcga gaccggccag
      601 ggtgatccac gcatcggtcg ggccgggcag gccgtcgaag tcacccatca ccacgtagtg
      661 atcgctgcgg aagaacgctc cgaagccgag cgcctcggct cgtcgcgcga cggcgagcaa
      721 ggtgtcgtag gtcgccccct gttggggctc ggtgaagata cggagatcgg ccagttgcat
      781 gtcccgcacc ctattggcgt gggccgcgag ggtggcgagt ggcgatgcgg tcgttcagcc
      841 gaggaggcgg cgcttggcct gttcgaactc ggcactcgac agcgcgccct gggcatgcag
      901 cgtggccaga cgctcgagct cgacagcggg attcatggcg ccagggctcg gcatccccat
      961 ggccggcgct gcgaaacctg cttgttgggg tgcgccgggg tagcccgcgg gcgcggcacc
     1021 ggccatgccg ccgggaaatg ccgtcgcgcc gccgagcgga ttggcgatac cgaactgagc
     1081 gaacagagcg gcaacctcgg gggtgaactg agtgaccatg gcgagggcga atttggtgtg
     1141 atcggccggg tcgaaccagg ccggccacac gtcgccgaca cgcggcaggt tgacctggct
     1201 gaccaccatc cgcttgctgg cttggaagcg gggcgagccg tcgagcggct ccagcatcac
     1261 gtgcaggtcg atgccgatgt tgatgttgtt gacgaccagg ccggtgggtt gggcaccgag
     1321 gatgtgggcc ctggccttgg tgcccgtggc ctgcaaccgg gcgatctcct gctgcttgtc
     1381 ggcggacggt cccatgccga gcgcggcgcg catgccgccg cccgccttgg ccacgctgcg
     1441 gaacacgacg aagatgatca gcaccgtcac cgcgaccgat ccaaccacga tccatgttgt
     1501 cgagcccatg gcgaccccct tcgtcattgc gtgcgccgac cgcggccaac ccacgaccga
     1561 tcgcaacgct acgcgccctc gtgattgcgg tcgaccaagt gggacctctt cgcgacccca
     1621 ccgacacccc ggcgctcggc gtcagccatc gaccccacga ggcagtgtgt tcagttcacc
     1681 ccgcgacgag gggtcaactc gacacggtcc acgtccctgt ggtcgacggt gttcgattca
     1741 ccccgcgacg aggggtcaac tcgacacggt cttcctggag ggatgcggcg gtgcgggcgg
     1801 tggatgcgtg gcgctggttc cggcgggcgg agtcatcggg caggatggcg gggtggtcgg
     1861 aggggagtcg tacgtcgtgg cgggcgtggt ctcactcgcc gtgctgcacg atggcgtcga
     1921 cgcagtggct gagcagatcg ccggggcggt cgagcggggc ggtttgctgg ggctcgccgt
     1981 gtcgcctgcg ggggtgggtt tggccgtagc cgcctcgacc gcgtgggccg tcgagaccga
     2041 cgaccccttg gcgctggtgg tggcgatcga gcgccgcgtg cgaccgcggt gggcgtggtg
     2101 gaacgcggca accgcgtcgt ggttgctggc cgagggcgtg cggatcgacc tgtgctggga
     2161 catcgccgcc gtgcaacgga tgctgtacgg cggctggcgg gccgaccccg ctcgcgtctn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngatgccg
     2341 aacagccggt gcagcccgac gggcacctcc gtcccgagtg ggcgctcgac gacggcactg
     2401 gccagggctg gtcggtgtcg gcccagcgcc tcgcccggtg ggcagctgcg gcgatggctg
     2461 ccgtcgagcg ccagggggca gcgatcgccg cgcaccccgc ccgcgcccgt ctggagcgaa
     2521 ccgtgcgctc cgagtcgagt gccgagctgc tcggtgccga gatggcccac ggcgggttgc
     2581 cgttcgacct cgccgcggca gagcacctga tcggttccct cgtcgggnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngatcgacg
     2761 tgcccgatac acgggcatgg cggttgaagc cgctgcgtgg tctgcacccg gtggtcgacg
     2821 cgctgctgac gtggcgcaag gccgagcgga tcgagaccac cttcggttac ggctggatcg
     2881 atcagcacgt gcgcggcggc cgcctgcggg gtgagtggag cggcagtgac ggtgccgctg
     2941 gacggatgac cgcttccgcg ggactgcaca acatgcccgc cgatctgcgc atcgccgtcg
     3001 cagccgaccc aggcttcacg ttcgtgcgtg ccgatctggg tcagatcgag ccgcgcgtgc
     3061 tcgccgcggt gtcgggtgat cccgcactga ccgccgcggc cgagcaggcc gacctgtacg
     3121 caccggttgc cgctcgcctc ggcgtcgagc gggcggtggc caaggtggcc gtgctggctg
     3181 cgatgtacgg ccagacgtcg ggcaccgcgg gcatggcgct gcaacggatg gagacggagt
     3241 atcccgtcgc cctgcagtat ctgcgcgacg ccgactcggc cggacgcgct ggacaggaca
     3301 tcaccaccta cggaggacgt ctggtgcgga tgtgggactt cggtgaccag tacgccgaac
     3361 agccgcaacg tgagcgagct gccgccgcgg ccaggggacg cttcgcccgc aacgcgatcg
     3421 tgcagggtgc ggcagcggag ttgttcaagg cgtgggcggt gatcgtgcgc gcccgggtcg
     3481 cgccgctcgg tgcggcggtc gtgctgtgcc tgcacgacga ggtgctggtg cacaccccgg
     3541 ccgaacacgc ggcggaggtc gccgagttga tcgagcgttg cctgcaggaa gccgcgtggc
     3601 gccacttcga cgacggcccc gcccgccggg tgcgcttcgt cgccgacgtc agcatcatcg
     3661 cccgctggag cgacgccaag gagtagccga cggatgtggt ttccgcggag tgcggaatgc
     3721 ccggatgcca gtggatcggc aatccgcgga agctggattc gccggacgta gaggcgagcg
     3781 aggccgggca tggctggaag gtgcccggag tagtgtcggg gccttggccc attggacgct
     3841 cgcgatcgac ttcggcacca gcttcacggt ggctgccgtg cgcgtcgaag ggcgcgcgcc
     3901 cgagatcgtc gagatcgccg gtgagcggcg tgtgccgtcg gtcgtgctgg tcgatccgag
     3961 cgggtcggtg ttcgtcggac gcaccgccga ggacctgtcg gtcacccacc ccggcaacac
     4021 cctgcgcgcc ctcaagaacc ggctcggtga tgcaacgccg gtgatcgtcg gtggacggcc
     4081 acaccaggtc gtcacgctgg tcgcggcact gctggccgag gtctattcgg cggtggtcac
     4141 gcagatgggc gagccgccga gcgaggtgcg tctcacccac cccgccacgt ggaaccagcc
     4201 gcggctgaac cgcctcatcg aagcggcggc caaggcgggt atgcccgagc ccttgctggt
     4261 gcccgagcct gtcgccgcgg cactgtcgta cgcagccgat gtgggtgtca gtgccggtcg
     4321 cccgattgcc gtctacgacc tgggtggcgg cacgttcgat tcggccgtcg tcgtcgcccg
     4381 tgagagcggg tgcgaggtcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngg
     4501 gatcagatcc aggtcggtga cgacgcgttg tggcgccagg tcggtgccgc cttgcgcaac
     4561 gaggtccgca aggccaaaga ggcgctgtcg tcgcacccgt acgcgaccgt gctggtgccc
     4621 ttgccgacgg gtctgaccca gttccgcctc acccgcgacg agttcgccgg gctgatcgag
     4681 acctacctcg acgagaccat ctcgatgatg cgccgcagcc tgcaggaagc cggagtcgct
     4741 ccggcagagc tggctggcat ctcgttggtc ggcg
//
LOCUS       DAORNF010000175         4772 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_13296_length_4772_cov_1.758109, whole genome shotgun sequence.
ACCESSION   DAORNF010000175 DAORNF010000000
VERSION     DAORNF010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4772
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13296_length_4772_cov_1.758109"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            117..>338
                     /locus_tag="PLV68_08180"
     CDS             117..>338
                     /locus_tag="PLV68_08180"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HRE01266.1"
                     /translation="MAELRRRRDEILEVARARGASRVRVFGSVARGDATTSSDVDFLV
                     DLEPGRSLFDLGGLLVDLQELLERDVDVVT"
     assembly_gap    339..384
                     /estimated_length=46
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <385..750
                     /locus_tag="PLV68_08185"
     CDS             <385..750
                     /locus_tag="PLV68_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HRE01267.1"
                     /translation="RGDRDRILDMIEMCDLLLGHASDHRRLAEDPVTQAAAQRWIEVL
                     GEAATHVSDEIKAAFPDVAWREVKGIRVILAHGYFHIEQDIVSNVVSNEVPRLRGQLQ
                     EVLDSLPEADVDEGGFTPE"
     gene            complement(923..1573)
                     /locus_tag="PLV68_08190"
     CDS             complement(923..1573)
                     /locus_tag="PLV68_08190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01268.1"
                     /translation="MGRWAGWSSTGWRRVTVAVAAAVAVVVWPGPLPTSVAHACSCFD
                     PAGIVPELQDKADAAFLGTVVEIRRPEVMLSSEAESRFIFDVETVFKGDTVRARQSIV
                     TASDGAACGLELSVGATAVVFARKQSDQVTPDDGEFASNLCEVIARPSPAVLSSLGEG
                     RPPVAGSSPIGADGSLASTLIRHWPWIVASLVAAAIAVVLTIRHRRHRAPARVNIR"
     gene            1634..2461
                     /locus_tag="PLV68_08195"
     CDS             1634..2461
                     /locus_tag="PLV68_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAC(3) family N-acetyltransferase"
                     /protein_id="HRE01269.1"
                     /translation="MGESEAIAETDRPVTTASLVADLRALGVVPGMTAMVHSSLSRLG
                     WVAGGAHAVVAALREAVGDEGTLMMPTHSSQLSDPAAWQNPPVPESWWAPIRAETPAY
                     DPALTPTEHMGRIVECFRHVPGVRRSAHPTVSAAAVGPNAAALIDGHQLAHGLGETSP
                     QARLYELDGHILLLGVTHANNTSLHLAENRAAPSNAAIATYGSPVMVDGHREWTAYPN
                     LVDSADDFERIGTAFASETGRQRTGPVGAGTAHLMHSRDVVDFAVDWLRVNRTWATH"
     gene            complement(2526..2984)
                     /locus_tag="PLV68_08200"
     CDS             complement(2526..2984)
                     /locus_tag="PLV68_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HRE01270.1"
                     /translation="MAERLAITRDIAASPEAVYAAITDIARMGEWSTECYSCEWHDGF
                     DTAVVGAVFDGHNRHGTAEWTSQATIVEADPGRSFVFHCSMFDFHFATWGYQIEPTDG
                     GCRVTEWTDDLRPESVLEMSKQMSGVDDRASHNATTMSTTLDRLAAALEP"
     gene            complement(3093..3165)
                     /locus_tag="PLV68_08205"
     tRNA            complement(3093..3165)
                     /locus_tag="PLV68_08205"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3130..3132),aa:Asn,seq:gtt)
     gene            complement(3262..>3818)
                     /locus_tag="PLV68_08210"
     CDS             complement(3262..>3818)
                     /locus_tag="PLV68_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRE01271.1"
                     /translation="ATWWIRQAITRSIADQGRTIRIPVHMVESMNRVLRVQRQLHQEL
                     EREPTFAELTAKVDLAPERVREILRMALDPLSLDSPVGEEDDSNLGDFIQDGSIDAPD
                     DAATKKMLVAAVDEALGELSEREQEIVRMRFGLDDGQARTLEEVGREFGVTRERVRQI
                     EAKTLAKLRHPQRSQKLKEFLDAE"
     assembly_gap    3819..3892
                     /estimated_length=74
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3893..4585)
                     /locus_tag="PLV68_08215"
     CDS             complement(<3893..4585)
                     /locus_tag="PLV68_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 factor domain-containing protein"
                     /protein_id="HRE01272.1"
                     /translation="MNQPDADAFAAPPPFPGVDRDKWAELVALGRSDGSVDVGQAVAA
                     LLDVELTGDTIALLKAHLAERGIALVDRDADQTAAPDLVDPATDEDDDGDGDGDGDGD
                     DGADRSPGAGDEPASKFSRRRLRRHERMAEKYETGNTADTVKMYLKEIGRVGLLSGDE
                     ERSLAAQIEAGVLAAARIDEMGPDGDPAEQRRLLRVVTAGQRAKNNLIQANLRLVVSI
                     AKRYAGRGMQLLD"
     gene            complement(4582..>4772)
                     /locus_tag="PLV68_08220"
     CDS             complement(4582..>4772)
                     /locus_tag="PLV68_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01273.1"
                     /translation="MNLVVAATRRTLAVRRRGVTPEEVREDAEARVHMEAVMSSNSPE
                     DAAEWLLGWLDRRKGERA"
BASE COUNT          738 a         1566 c         1564 g          784 t
ORIGIN      
        1 tcgtgggttc gatccccacc cgccccacca acagcttgtg ttacttcggc tgattcctct
       61 ctgtggtcgg tgccgccgga tccaacgaat atcctgtcgg cgtgggtacg accagcatgg
      121 ccgagctgcg gagacggcgc gacgagatct tggaagtcgc gcgggcgcgg ggtgcctcac
      181 gcgtcagggt ctttggttcc gtcgcgcgcg gtgacgcgac gacgtcgagc gacgtcgact
      241 tcctcgtcga ccttgagccg ggccgaagcc tgttcgatct gggtggcttg ctcgtcgacc
      301 ttcaagaact tctcgaacgc gacgtggacg tcgtgacgnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnaggggc gaccgcgacc ggattctcga catgatcgag
      421 atgtgcgacc tgttgctcgg gcacgcttcg gatcaccgtc ggcttgctga ggatccagtc
      481 acacaggcgg ccgcgcaacg atggatcgag gtgctcggcg aggcggcgac gcacgtgtcg
      541 gacgagatca aggcggcgtt ccccgacgtt gcctggcgcg aggtcaaggg catcagggtg
      601 atcctcgccc acggctactt ccacatcgag caggacatcg tcagcaacgt ggtcagtaac
      661 gaggtcccga gactgcgcgg tcagctgcag gaggttcttg actcgttgcc tgaggctgac
      721 gtcgacgaag gcggattcac acccgaataa ctcgcaccac agcgctcgtc ggcgcaggcg
      781 aactacctgg tagatcgcag ttttctacgg tgctcggtcg gtgaagttct cataatcccc
      841 ggagcgtggg ttcgatcccc cccgccccac caacagcccc tgatcgcgac gtcggtcgcg
      901 ggtcggttcg gtgtcccggt cgtcaacgta tgttgacccg tgccggggcc cggtggcggc
      961 ggtggcggat tgtgagcacc actgcgatcg cggctgcgac gagggacgcg acgatccagg
     1021 gccagtggcg gatgagcgtc gatgccaggc tgccatcggc tccgatcgga gatgagccag
     1081 caaccggcgg gcgaccttcg ccgagggagc tcaacacggc gggcgagggt cgagcgatca
     1141 cctcgcacag gttcgatgcg aactcgccgt cgtcaggtgt cacctggtcg ctctgcttgc
     1201 gcgcgaagac gacggcggtc gctccgacgg atagctcaag tccgcacgcg gcaccgtcgc
     1261 tggccgtcac gatcgactgc cgtgcccgaa ccgtgtcgcc cttgaacacc gtctcgacat
     1321 cgaagatgaa acgcgactcg gcctcggagg acagcatcac ctccggtcgg cggatctcga
     1381 caaccgtgcc gaggaatgcc gcatccgcct tgtcctgaag ctcgggaacg atcccggcag
     1441 gatcgaagca actgcatgca tgcgcgaccg acgtcggcaa cggccccggc cagaccacga
     1501 cggcaacggc ggctgcaaca gccacggtga cgcgccgcca acctgtcgac gaccaaccgg
     1561 cccaacgccc catgacgccc atcatcgcgc gagcgagtgc gtcgtcgccg acgtcgcgtg
     1621 gaaggctgag gtgatgggtg agtcggaggc gattgcggag acggatcggc ccgtgacgac
     1681 ggcgtcgttg gtcgccgatc tgcgcgcgct cggcgtggtg ccggggatga ccgcgatggt
     1741 gcactcgtcg ctgtcgcggc tgggctgggt ggcaggcggg gcacatgctg tcgtcgccgc
     1801 gttgcgcgag gccgtgggtg acgagggcac gttgatgatg ccgacgcact ccagccaact
     1861 ctccgaccct gccgcgtggc agaacccgcc ggtcccggag agttggtggg caccgatccg
     1921 ggccgagacc ccggcctacg atccagcgct gaccccgacc gagcacatgg ggcggatcgt
     1981 ggagtgcttc cgccacgtcc caggcgtacg gcgtagcgct catccgaccg tgtcggctgc
     2041 tgcggtaggt ccgaatgccg cggcgctcat cgacgggcac caactcgctc acggactcgg
     2101 cgagacgtca ccacaagcgc gcctgtacga actcgacggc cacatcctgc tgctcggggt
     2161 gacccacgcc aacaacacgt cgctgcatct ggccgagaac cgcgccgcgc ccagcaacgc
     2221 ggcgattgca acctacggtt cgcccgtcat ggtcgacggg caccgcgagt ggacggcgta
     2281 ccccaacctg gtcgacagcg ccgacgactt cgagaggatc ggcacagcgt tcgcgtccga
     2341 gacgggacgg caacgaaccg ggcccgtcgg tgctgggacg gctcacctga tgcattcccg
     2401 cgatgttgtc gacttcgccg tcgactggct ccgcgtcaac cgcacatggg cgacccactg
     2461 acctgcgaca atcggccgat gcggacggtg ctggtgaccg acgcgcgtgg ggcgcggtgc
     2521 tcgcgtcagg gttcgagggc ggcggcgagg cggtcaaggg tggtggacat ggtggtggcg
     2581 ttgtggctgg cacggtcgtc gacgccggac atctgcttgc tcatctccag caccgactcg
     2641 gggcgcagat cgtcggtcca ctcggtgacc cggcagccgc cgtcggtcgg ttcgatctgg
     2701 taaccccacg tggcgaagtg gaagtcgaac atgctgcagt ggaacacgaa cgaccggccc
     2761 gggtcggctt cgacgatggt ggcctgcgag gtccactcgg cggtgccgtg acggttgtgg
     2821 ccgtcgaaca cggcgccgac gaccgctgtg tcgaatccgt cgtgccactc gcacgagtag
     2881 cactcggtcg accactcccc catgcgcgcg atgtcggtga tcgcggcgta gaccgcttcg
     2941 ggtgatgctg cgatgtcgcg ggtgattgca agccgttctg ccatggccga cagtccacca
     3001 cgctgacggg gcgtcagccg tgtcgcagcg tcagcgtgtc gggcgaaaac gagacgtggg
     3061 gtccgaccgg atggcggacc ccacatcgtg cgctccgaga gcagggctcg aacctgcgac
     3121 ccgctgatta acagtcagct gctctgccaa ctgagctatc tcggaaaggc acccgcagat
     3181 taacagcgag ccgagacagt tccacggccg atacgcgtca cgcgacgagc ccaccgcgcg
     3241 cggggcgacg cggcgtccca actactcggc gtcgaggaac tccttcagct tctggctgcg
     3301 ctgcgggtga cgcagcttgg ccaacgtctt ggcttcgatc tggcgcaccc gctcgcgtgt
     3361 gacgccgaac tcgcgcccga cctcttccag cgtgcgcgct tgaccgtcgt caaggccgaa
     3421 gcgcatccgc acgatctcct gctcgcgttc gctcaactcg ccaagcgcct cgtcgaccgc
     3481 ggcgacgagc atcttcttgg tcgcagcatc gtcgggggca tcgatcgaac cgtcctggat
     3541 gaaatcaccc aggttcgagt cgtcttcctc accgaccggc gagtcgagcg acagcgggtc
     3601 gagcgccatc cgcaggatct cgcgcacccg ctcgggagcg agatcgacct tggcggtcag
     3661 ttcggcgaac gtgggttcac gctccaactc ctgatgcagt tggcgttgca cgcgcaggac
     3721 ccggttcatg ctctcgacca tgtgcaccgg aatgcggatc gtgcggccct ggtcggcgat
     3781 gctgcgggtg atcgcctggc gaatccacca ggtggcgtnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngtcgagca
     3901 gctgcatgcc ccgccccgcg tagcgcttgg cgatgctgac caccagacgc aggttggcct
     3961 ggatgaggtt gttcttggcc cgctgcccgg cggtgaccac gcgcagcagc cgccgctgct
     4021 cggccgggtc gccgtcggga cccatctcgt cgatgcgcgc cgcggccagc acgccggctt
     4081 cgatctgggc cgcgagcgac cgctcctcgt caccgctgag caaaccgacc cggccgatct
     4141 ccttgaggta catcttgacg gtgtcggccg tgttgcccgt ctcgtacttc tcggccatcc
     4201 gctcgtggcg gcggagccgg cggcgtgaga acttgctggc cggctcgtcg ccggccccag
     4261 ggctgcgatc ggcaccatcg tcaccgtcgc cgtcaccgtc gccgtcaccg tcgtcgtcct
     4321 cgtcggttgc tggatcgacc agatctggcg ccgctgtctg gtcagcatcg cggtcgacca
     4381 acgcgatacc gcgctcggcc aggtgtgcct tgagcagggc aatggtgtcg ccggtgagct
     4441 cgacgtcgag cagtgcggcg accgcctggc cgacgtcgac cgagccgtcg ctccggccga
     4501 gcgccaccaa ctcggcccac ttgtcgcggt cgacaccggg aaacggcggg ggcgctgcga
     4561 aggcgtccgc atctggctga ttcatgcgcg ctcccccttt cgccggtcga gccaccctag
     4621 caaccactca gcggcgtctt ccggcgagtt ggagctcatc acggcttcca tgtgaacccg
     4681 cgcctcggca tcttctcgca cctcctcggg tgtgacgcct cgcctgcgaa cggcgagtgt
     4741 gcgccgagtt gcagccacga ccaggttcat cg
//
LOCUS       DAORNF010000176         4769 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_13307_length_4769_cov_1.745439, whole genome shotgun sequence.
ACCESSION   DAORNF010000176 DAORNF010000000
VERSION     DAORNF010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4769
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13307_length_4769_cov_1.745439"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>231)
                     /locus_tag="PLV68_08225"
     CDS             complement(<1..>231)
                     /locus_tag="PLV68_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01274.1"
                     /translation="WGEPLATLRRIQDEINRTFNEPQGAQAEYPPLNVWRGEAAVIVT
                     AEIPGVRLEDMELTVHQNTLTIKGRRELVAKEP"
     assembly_gap    232..376
                     /estimated_length=145
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            456..>847
                     /locus_tag="PLV68_08230"
     CDS             456..>847
                     /locus_tag="PLV68_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01275.1"
                     /translation="MSLRRTVLAAAALLSAAGMPAAAAAQAISLAELGRTLNIDPEQV
                     SVSGVSSGGFMAHQFHVAYSRRIIGAGIVAAGPYGCASARSGLAWFDPSGLFTSLYVC
                     SAINPLGLYLGPPDVQHSLARTQREAEAG"
     assembly_gap    848..992
                     /estimated_length=145
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <993..1572
                     /locus_tag="PLV68_08235"
     CDS             <993..1572
                     /locus_tag="PLV68_08235"
                     /inference="COORDINATES: protein motif:HMM:NF021982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HRE01276.1"
                     /translation="DAVVFQQHPVANHAMITEDFGNPCAADGPPYINDCDLDVAFALL
                     QHIYGPAPLQPAAGALEAVQRFDQRPFFDAADARVSLHASGHVYVPARCRQGARCRLH
                     VAFHGCRQSEDEIGDAFYGGAGYNAIAESNAIVVLYPQVRAWTASWFQQYRENPRGCW
                     DWWGYSGADYALKSGKQMRAVAAMIAALTGKD"
     assembly_gap    1624..1633
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1680..>2026)
                     /locus_tag="PLV68_08240"
     CDS             complement(1680..>2026)
                     /locus_tag="PLV68_08240"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HRE01277.1"
                     /translation="LVLFCIGKKTSCLAACAQGSAFIVNVLSEQQKHLSEAFARKEHD
                     KFAGVDFTIGGNGCRVLTGCLATLECSRVDTYEGGDHLIVIGRVERMALADEAAPLLR
                     YCARYHRIGAEA"
     assembly_gap    2027..2127
                     /estimated_length=101
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2364..2714
                     /locus_tag="PLV68_08245"
     CDS             2364..2714
                     /locus_tag="PLV68_08245"
                     /inference="COORDINATES: protein motif:HMM:NF014666.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01278.1"
                     /translation="MLFAVGILVVVGSVIGGVRVRHIDDLSQIIQSRRVSIGVVATPP
                     SAAQDAADRLVRAGITSVLNFAPVVLAVPHGINVRKVDLAVELQILSYHEQRRTPIGL
                     HAVGSNEAAREASA"
     gene            2686..4005
                     /locus_tag="PLV68_08250"
     CDS             2686..4005
                     /locus_tag="PLV68_08250"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:NF000744.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HRE01279.1"
                     /translation="MRRRERPARRLHPERKASPTVSIVVIGVNHRTGPIDVLERVAVS
                     VESGPKALTGLISRPYVRETVLLSTCNRTEVYVVAERFHGAYADIRDFFCELGGLTPD
                     ELHPHLYSQHDHGAITHLFEVAAGLDSAVLGEHEILGQVRGAWQLAQREGGSKSTLNL
                     LFRHAVETGKRVRTDTAIGRGTASVSHAAVEMATEHLGSLHAKRVLVVGAGEMGEGVA
                     VALASAGAASITVINRTEERAMQLAERVGGGVAHFSALSDAIAVADVVVTCTGSGTLV
                     LDHDAVAAARHTAANPLLIVDIAVPRDVDASVGTLDGVTVLNLDDLRDWAARGVEQRW
                     AEAEAVRGIVAEEVERFQIELTSRQAAPLVAQLHQRADSIRQSELDRFAPRLASLDAG
                     QREAVEAVTRGIVAKLLHAPSVRLKGDSGTPSGERNAAAVRDLFDLT"
     gene            4025..>4769
                     /gene="hemC"
                     /locus_tag="PLV68_08255"
     CDS             4025..>4769
                     /gene="hemC"
                     /locus_tag="PLV68_08255"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HRE01280.1"
                     /translation="MTVRVATRSSAQARTQAQFVADAIAAANQGVATELVFVDTVGDR
                     RQDVPLHTIGGQGVFVKEVQRAVLDGRADIAVHSAKDLPSAPAEGLTIAAFTARRDAR
                     DALVGSTLDGLALGATVATGSVRRRAQLAAMRPDLRFVELRGNIQTRLGKLPPNGALV
                     MAVAALQILGLDDQVAQAMDLDAMVPAVGQGCVAVECRSDDPTVAALVAAVDHAPTRR
                     AVEAERAFLAQLGSGCSLPVGAHVVDGQLL"
BASE COUNT          656 a         1499 c         1469 g          744 t
ORIGIN      
        1 ggttccttcg ccacgagttc gcgccggccc ttgatggtca gcgtattctg gtgaaccgtc
       61 aactccatat cctccagccg cacgccaggg atctcggcgg tgacgatcac cgcggcctcg
      121 ccgcgccaga cgttgagcgg cggatactcg gcttgcgcgc cctgcggctc attgaacgtc
      181 cggttgatct cgtcctggat gcggcggagc gtcgccaggg gttctcccca cnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnccgc tgcccgcgcg gccctggcgg acgtggcgtc actcgcgagg
      421 tctcaatgcg atcgcatggt cgaacatcgt ggcacgtgtc cttgcggagg acggtgctgg
      481 cagcggcggc gctcttgtcc gcggctggca tgcccgcggc tgcggccgcg caggcgatct
      541 ccctcgccga gcttggccgg acgctcaata ttgatccgga acaggtttcg gtttccggcg
      601 tttcgtccgg gggcttcatg gcgcaccagt tccacgttgc ctattcccgg cgcatcatcg
      661 gcgccggaat tgtcgctgcc ggcccctacg gctgcgccag cgcgcgctcg ggccttgcct
      721 ggtttgatcc gagcggcctg ttcacgtccc tctatgtctg ctcagccatc aacccgctgg
      781 gcctttacct cggcccgccg gacgtgcagc actcccttgc ccgcacgcaa agggaagccg
      841 aggccggnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnggacgcgg tcgttttcca gcaacatccg
     1021 gtcgccaacc acgccatgat caccgaggat tttggcaatc cctgtgccgc cgacggcccg
     1081 ccctatatca acgattgcga tctcgacgtt gctttcgcct tgctgcagca catctatggt
     1141 ccggcgccgt tgcagccagc ggcaggcgcg cttgaggccg tgcaacgttt cgaccagcgg
     1201 cccttcttcg acgcggcgga tgcgagggta agcctgcatg cgagcgggca cgtctacgtg
     1261 cccgcccggt gccgccaagg cgcccgctgc cggctgcatg tcgccttcca tggctgccgg
     1321 caaagcgagg acgagatcgg cgacgcgttc tatggcggtg ccggctacaa cgcgatcgcc
     1381 gagagcaatg cgatcgttgt tctctaccct caggtcaggg cctggacggc gagctggttc
     1441 cagcagtacc gcgaaaatcc gcgtggttgc tgggactggt gggggtattc cggcgccgat
     1501 tatgcgctga aatccggcaa gcagatgcgg gccgtggcgg cgatgattgc ggcacttacc
     1561 ggcaaggatt gatggcgacg gtcagtgcga accgcttatc gccgaaccgg cttcgtggct
     1621 atcnnnnnnn nnnggcgccg ccgccatcgc cgccgccgcc gccggcaccc ctgcctcgtc
     1681 tacgcctcag caccgatccg gtggtagcgg gcgcaatagc gcaacagcgg cgcggcttca
     1741 tccgcgagcg ccatgcgctc gacgcggccg ataacgatga ggtggtcacc cccctcatac
     1801 gtgtcgaccc ggctgcactc aagcgtggcg aggcagcccg tcaggacgcg gcagccattg
     1861 ccgccgatcg tgaagtcgac gccggcaaac ttgtcatgct ccttgcgcgc aaaagcctcc
     1921 gacaggtgct tctgctgctc cgagaggacg ttgacgataa aggcgctccc ctgggcgcag
     1981 gccgcaagac acgacgtctt cttgccgatg cagaacagca ccagcgnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnagc gaaacggctt agcgccgtgc gaaatcgagc
     2161 gtccgcgtca gtcatgttcc accctcaaac agcgccatcc cgccgcccgc catcgtttgg
     2221 atttcgttca ttcttgttaa caactcgtta tagttgcgct ttctcggcat agatgctact
     2281 ttatgctgca tgaggtgctc gcccgcgcgg cgagtaccta cgagacgcca tctcggtccg
     2341 tcaagggagt cgaaggtccg gccatgctgt ttgcagtcgg cattctggtg gttgtcggca
     2401 gcgtcatcgg cggtgtgcgc gtgcgccaca tcgacgacct gtcccagatc atccagtcgc
     2461 gtcgggtgag catcggcgtg gtggccacgc caccgagcgc ggcgcaggac gcggccgacc
     2521 ggctggtgcg ggccgggatc accagcgtcc tcaacttcgc gccagtggtg ctggccgtcc
     2581 cgcacggaat caacgtgcgc aaggtcgacc ttgccgtcga gctccagatc ctcagctacc
     2641 acgagcagcg ccgcaccccg atcggcctgc atgccgtcgg gtcgaatgag gcggcgcgag
     2701 aggccagcgc gtaggctgca tcccgagcga aaggcatctc ccactgtgtc catcgtcgtc
     2761 attggtgtga accaccgaac cggtccgatc gacgtgctcg aacgcgtcgc ggtgtcggtc
     2821 gaatcgggcc ccaaagcact gaccgggctg atcagccgtc cgtacgtgcg cgagacggtg
     2881 ctgctcagta cgtgcaaccg caccgaggtc tacgtggtgg ccgagcggtt ccatggtgcc
     2941 tacgccgaca tccgtgactt cttctgcgag ctcggtgggt tgacgcccga cgagttgcac
     3001 ccgcacctct acagccagca cgaccacggt gcgatcaccc atctgttcga agtggccgcc
     3061 gggctcgatt cggccgtgct cggcgagcac gagatcctcg gccaggtgcg cggcgcctgg
     3121 cagcttgccc aacgggaggg cggctcgaag tcgaccctca acctgttgtt ccgtcacgcc
     3181 gtcgagaccg gcaagcgggt gcgcaccgac acggccatcg gccgtggcac ggccagcgtc
     3241 agccacgctg cggtcgagat ggccaccgaa cacctcggct cgctgcacgc caagcgggtg
     3301 ctggtggtgg gcgccggtga gatgggcgaa ggggtcgccg tcgcgctggc cagcgccggt
     3361 gctgcgtcga tcacggtcat caaccgcacc gaagagcgag cgatgcaact cgccgagcgc
     3421 gtcggtggtg gcgtggccca cttctctgcg ttgtccgatg ccatcgcggt cgccgacgtg
     3481 gtcgtcacct gcaccgggtc aggcacgctc gtgctcgacc acgacgcggt tgccgcggcg
     3541 cgccacacgg cagccaaccc gctgctcatc gtcgacatcg ccgtaccgcg cgacgtcgat
     3601 gcctcggtcg gcacgctcga cggggtgacg gtgctgaatc tcgacgacct gcgtgactgg
     3661 gcggctcggg gcgtcgagca gcgctgggcc gaggccgagg ctgtgcgcgg catcgtggcc
     3721 gaagaggtcg agcggttcca gatcgaactc acgtcccgcc aggccgcgcc gctggtggcc
     3781 cagttgcacc agcgggccga ctccatccgt cagtccgagc tcgatcggtt cgcgccgcgt
     3841 ctggccagcc tcgacgcggg ccagcgtgag gcggtcgaag cggtcacccg gggcatcgtc
     3901 gccaagctgc tgcacgctcc cagcgtgcgg ctcaagggtg acagcggaac gcccagtggc
     3961 gagcgcaacg cggccgcggt gcgcgacctg ttcgacctga cctgagccga ccatgccaat
     4021 ggggatgacg gtcagggtcg ctacccggtc gagcgcccag gcgcgcacgc aagcacagtt
     4081 cgtcgccgat gcgatcgctg ctgccaacca gggcgttgcc accgagttgg tgttcgtcga
     4141 cacggttggc gaccggcgcc aagacgtgcc cctgcacacc atcggcggcc agggtgtgtt
     4201 cgtcaaggaa gtgcagcggg cggtgctcga cggccgggcc gacatcgccg tgcacagtgc
     4261 caaggacctg ccgtcggccc cggccgaggg gttgacgatc gctgcgttca ccgctcggcg
     4321 cgacgctcgc gacgcgttgg tcggttccac cctcgacggc ctcgcgttgg gagccacggt
     4381 cgccactggt tcggtgcggc ggcgggcgca actcgcggcg atgcgcccgg acctgcgctt
     4441 cgtcgagctg cgcggcaaca tccagacccg gctcggcaag ctgccgccaa acggcgcgct
     4501 ggtgatggcg gtcgcggcgc tgcagatcct cggtctcgac gatcaggtgg cccaggcaat
     4561 ggacctcgac gcgatggtgc cagcggtcgg ccagggctgc gtggcggtcg agtgccgcag
     4621 cgacgacccg accgtggccg cgctggtcgc ggcggtcgac cacgcgccca cccgccgggc
     4681 ggtcgaagcc gagcgcgcgt tcttggccca actgggctcg ggttgctcgc tgcccgtcgg
     4741 cgcgcacgtg gtcgacggcc aactgctgg
//
LOCUS       DAORNF010000177         4761 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_13336_length_4761_cov_1.438802, whole genome shotgun sequence.
ACCESSION   DAORNF010000177 DAORNF010000000
VERSION     DAORNF010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4761
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13336_length_4761_cov_1.438802"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /locus_tag="PLV68_08260"
     CDS             <1..525
                     /locus_tag="PLV68_08260"
                     /inference="COORDINATES: protein motif:HMM:NF039673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6157 family protein"
                     /protein_id="HRE01281.1"
                     /translation="TTDSSVHLFVFDRNPSTEKVSLSPGRMHRTRGRARLGRVGTTNY
                     SDTFIQVADDCPVAAGEQPPDSGKGPAVAALQFAVISANPYALTSDDVLFEVHARRNG
                     IPSAARTAARAAFFATSQACLRASPLAKRYGWGLHHDAESRVALVPLGSDEYQRLVND
                     PSVKQVKAMRSKRV"
     assembly_gap    593..743
                     /estimated_length=151
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <744..1023
                     /locus_tag="PLV68_08265"
     CDS             <744..1023
                     /locus_tag="PLV68_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01282.1"
                     /translation="ADRMAADETDSATSSETGSAADEEESLTAQGPLLAARDIAVFVV
                     GFVVVCAGLWSGSNAVVIAGAVLALVGVVVPLRSLARRGRTAGAESMP"
     assembly_gap    1138..1404
                     /estimated_length=267
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1405..1622
                     /locus_tag="PLV68_08270"
     CDS             <1405..1622
                     /locus_tag="PLV68_08270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01283.1"
                     /translation="DETAPDKRAPDEIAPDEVTVGDIRPLGGHDADETQAMPWRFSFD
                     QDDDLGGLLHTDSPLLSRAFRGKRPPR"
     gene            1689..2102
                     /locus_tag="PLV68_08275"
     CDS             1689..2102
                     /locus_tag="PLV68_08275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HRE01284.1"
                     /translation="MELIADLRAPCRADELFGWIDDLERYPQWMGLVHGARSVPSQPG
                     EGLAWDVELRARVGPFARSKRLRMVRTVHEIDRGAVFERHELDGRSHSTWRLTAQIEE
                     DPDQGGCTLEMGLHYGGSLWTGGCCTTRSRPAAPT"
     gene            complement(2103..>2406)
                     /locus_tag="PLV68_08280"
     CDS             complement(2103..>2406)
                     /locus_tag="PLV68_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01285.1"
                     /translation="VADPSAPPLVLVTHHVDEVPAGMTHALLLREGRPLAAGPIDQVL
                     TADNLSTCFGLPLSLERRPDGRLAAWSLASASLASAPLAAASATDGSLTAGSRPAP"
     assembly_gap    2407..2432
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2433..3014)
                     /locus_tag="PLV68_08285"
     CDS             complement(<2433..3014)
                     /locus_tag="PLV68_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRE01286.1"
                     /translation="MARTPHQRHAAGTGPDQEAVLALDHVSFVRDGRAILDDVTWHVH
                     ADERWLVLGANGSGKTSLVRIASMYEHPSSGTVRVLGETLGRTDVRVLRRRVGLMSAA
                     LAAQLRPALSAIEIVVTARYAALEPWWHRYTADDMAEAQACLDRMGVGHFAERSLGTL
                     SSGEQQRVLLARSLMNDPGVILLDEPSARLDLGG"
     gene            3194..>3452
                     /locus_tag="PLV68_08290"
     CDS             3194..>3452
                     /locus_tag="PLV68_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01287.1"
                     /translation="MPKYRRLFAALALTVVTLHGSGALAQAATDPTLPAGETAEDAAK
                     AIIAARKRANEAADAWVQAHSRLDGLRDEKASIERDIADLQR"
     assembly_gap    3453..3476
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3477..>3935
                     /locus_tag="PLV68_08295"
     CDS             <3477..>3935
                     /locus_tag="PLV68_08295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01288.1"
                     /translation="LQESVAKVAVSRYLGTNKTGIDLLVGNLGPTEQAQAVVLSGVVN
                     DVSEGVMDRYEALDLRLDNRIADLADSERRVQAAADEFDKRQAEAEAEVARLKAIEKD
                     RLEIEAVRRALEAQRQREVEALQREMAEQLRRDQDAAIAFQEGGGSDGGD"
     assembly_gap    3936..4064
                     /estimated_length=129
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4065..4416
                     /locus_tag="PLV68_08300"
     CDS             <4065..4416
                     /locus_tag="PLV68_08300"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRE01289.1"
                     /translation="FGDSWGMPRSGGRLHQGVDMIGERGLPLVAVVDGLAVARQNTLG
                     GTTISFYGTDGNRYYYAHLDNYGQLGQVVKGTVIGYLGQTGNAIQSVPHLHFEIHPGG
                     GAAVNPYATVAANC"
     gene            4494..>4761
                     /locus_tag="PLV68_08305"
     CDS             4494..>4761
                     /locus_tag="PLV68_08305"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HRE01290.1"
                     /translation="MAHHASGDSDLGRQLAAALGAGQVTELRQLSGGASRETWLFTAD
                     GRRLVLQRQRSGDTRDMATEVAVLQAAHRGGVPVAEVIASSSAAT"
BASE COUNT          681 a         1379 c         1495 g          609 t
ORIGIN      
        1 actacagaca gtagcgtaca cctgttcgta ttcgatcgca acccttcaac agagaaagtt
       61 tcgttatcgc cgggtcgcat gcacaggacc cgcgggcggg ccagactggg acgcgtgggg
      121 acgacgaact actcggacac gttcatccag gtggcggacg actgcccggt tgcggcgggt
      181 gagcagcctc cggacagcgg caagggtccg gcggtggccg cgttgcagtt cgcggtgatc
      241 tccgccaacc cctacgcgct cacgagcgac gacgtgctgt tcgaagtaca tgcgcgacga
      301 aacgggatcc cgtcggccgc gcggaccgcg gcacgagccg cgttcttcgc cacgagtcag
      361 gcgtgtcttc gagcgtcgcc gcttgcgaag cggtacggct ggggtctgca tcacgatgcc
      421 gagagccgcg tcgccctggt accgctcggc tccgacgagt accagcgcct cgtcaacgac
      481 ccgtcggtga aacaggtgaa agcgatgcgc tccaagcgag tgtgacggcg cgctggcgga
      541 cggagaggtg gccttggatc acgtcgtcac aggtcgatat ccgtaccgaa tcnnnnnnnn
      601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nnncgctgac cggatggccg ccgacgagac cgacagcgcg
      781 acttccagcg agaccggctc agcagccgat gaggaagagt cgctcaccgc gcaggggccg
      841 ttgctggccg cgcgggacat cgccgtgttc gtggtgggtt tcgtcgtcgt gtgcgccggg
      901 ctgtggtcag gttccaacgc ggtggtgatc gccggcgcgg tgctcgcgct ggtgggggtc
      961 gtcgtgccgc tgcgctcgtt ggcccgacgg ggccgaaccg ccggggccga gagcatgccg
     1021 tagtacggtt ggcccgtggc ccgactgtgc gaacgtccag gatgttccgc cgttgccacg
     1081 gtcgcctacg gcatggacgg cgaccagttg ctggtgtggc tcgcagcagc ccctcccnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnncggacg agacggcgcc ggacaagagg gcgccggacg
     1441 agatcgcacc ggacgaggtc acggtgggcg atatccgtcc gttggggggc cacgacgccg
     1501 acgagaccca ggcgatgcca tggcggttct cgttcgacca ggacgacgac ctcggtggtc
     1561 tgctccacac cgacagccca ctgctgtcgc gggcgttccg gggcaaacgc ccaccacgct
     1621 gacaacgcct cgctgaatca aggaccgagt gcgccgcggg ccatactggc cgtcatggag
     1681 ttgtcgggat ggagctgatc gccgatctgc gcgcgccgtg ccgggccgac gagctgttcg
     1741 gctggatcga cgacctcgag cgttacccgc agtggatggg cctggtccac ggcgcgcggt
     1801 cggtgccgtc gcagccgggt gagggtctgg cgtgggacgt cgagctgcgc gcccgggtcg
     1861 gcccgttcgc ccgctcgaag cggctgcgga tggtacgcac ggtgcacgag atcgaccgcg
     1921 gcgcggtgtt cgaacggcat gagctcgacg ggcgatcgca cagtacgtgg cgactgaccg
     1981 cgcagatcga ggaggaccct gaccagggtg ggtgcaccct cgagatgggg ctgcactacg
     2041 gcggatcgct ctggaccggc gggtgctgca cgacgagatc gaggccagcc gcgccaacct
     2101 gatcagggtg ctggccgaga cccggctgta agcgacccgt ccgtcgccga cgcggctgcc
     2161 agcggcgcgc tggcgaggga cgcgctggcg agggaccagg ccgccaggcg accgtcgggg
     2221 cggcgctcca acgacagcgg gagcccgaag caggtgctga ggttgtccgc ggtgagcacc
     2281 tggtcgatcg gaccggctgc gagaggacga ccctcgcgga gcaacaacgc atgggtcatg
     2341 cccgctggta cttcgtcgac gtgatgggtg acgagcacca gcgggggagc gctgggatcc
     2401 gcgaccnnnn nnnnnnnnnn nnnnnnnnnn nncccgccaa gatcgagccg ggcgctgggt
     2461 tcgtcgagta ggatcactcc gggatcgttc atcagcgagc gggccagcag cacgcgctgc
     2521 tgttcgccgg acgacaacgt gcccaggctc cgttcagcga agtggccgac acccatgcgg
     2581 tcgagacacg cttgagcttc ggccatgtcg tcggcggtgt agcggtgcca ccatggttcg
     2641 agcgccgcgt agcgggcggt gaccacgatc tcgatcgcgc tcagcgccgg gcgcagttgg
     2701 gcggccagcg cagccgacat gagcccgacg cggcggcgca gcacgcgcac gtcggtgcgc
     2761 cccaacgtct cgcccaggac tcgcaccgtg cccgacgacg ggtgttcgta catcgacgcg
     2821 atccgcacca gactcgtctt gccgcttccg ttggccccga gcaccaacca tcgctcgtcg
     2881 gcgtgcacgt gccacgtgac gtcgtcgagg atcgcccggc cgtcacgcac gaaggagacg
     2941 tggtcgagtg ccagcactgc ctcctggtca ggaccggtgc cggcggcgtg ccgctggtgg
     3001 ggtgtgcgcg ccatgcccgg ccaccatacc gatccggtcg cgacgaccga acatgctccg
     3061 gaacggtgct ccagaaccct gctccagaac acggcgcgat cggccgaaac acaacgacgt
     3121 ggacagtacg gcggtagcga gacgacgggt gagcggccaa ccgcgacggg tgctggtaga
     3181 ttccgtcgcc agcatgccga agtaccgtcg cctcttcgct gcgctcgccc tcaccgtcgt
     3241 gacgctgcac ggttccggcg cgttggcgca ggcggcaacc gaccccacgc tgccggctgg
     3301 cgagacggcg gaagacgccg ccaaggcgat catcgcggcc cgcaagcgtg ccaacgaggc
     3361 tgccgatgcg tgggtgcagg cgcacagtcg tctcgacggg ctgcgcgacg agaaagcgtc
     3421 gatcgagcgc gacatcgccg acctccaacg gcnnnnnnnn nnnnnnnnnn nnnnnncact
     3481 gcaggagtcg gtcgccaagg tcgcggtcag ccgctacctc ggcacgaaca agaccggcat
     3541 cgacctgctc gtgggcaatc tcggcccgac cgagcaagcc caggccgtcg tgctgtccgg
     3601 tgtggtcaac gacgtctccg aaggagtgat ggaccggtac gaagccctcg acctgcgact
     3661 cgacaaccgc atcgcggacc tggccgacag cgagcgccgg gtgcaggccg cggccgacga
     3721 gttcgacaag cgccaggcag aggccgaagc cgaggtcgct cgactgaagg cgatcgagaa
     3781 ggaccgcctc gagatcgaag cagtacgccg ggcgctcgaa gcgcaacgcc agcgcgaggt
     3841 cgaggcgctt cagcgcgaga tggccgagca actgcgccgc gatcaggacg ccgcgattgc
     3901 cttccaagaa ggcggcgggt ccgacggcgg cgacgnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncttcgg cgacagctgg
     4081 ggcatgcctc gctccggcgg tcgtctgcac caaggcgtcg acatgatcgg cgagcggggg
     4141 cttcctctgg tggccgtggt cgacggcctt gccgtggccc gtcagaacac cttgggtggc
     4201 acgacgatct cgttctacgg caccgacggc aaccgctact actacgccca cctcgacaac
     4261 tacggccaac tcggccaggt ggtcaagggc accgtgatcg ggtacctcgg ccagaccggt
     4321 aacgcgatcc agtcggtgcc gcacctccac ttcgagatcc accccggtgg cggcgccgcg
     4381 gtcaatccgt atgccacggt ggccgcgaac tgctgacggc gaactgctgc cgatcgggct
     4441 ggctgtcgtt cgccgactgt ggatgtcggg ggcggctgtg cgagattgac cccatggcgc
     4501 accatgcctc tggggacagc gacctcggtc gccagcttgc tgcggcgctc ggcgctggcc
     4561 aggtcaccga acttcgtcag ctgtcaggcg gcgcgtcgcg agagacgtgg ctgttcacgg
     4621 ccgacgggcg gcggctggtg ctgcaacgcc aacgcagcgg cgacacgcgc gacatggcga
     4681 ccgaggtcgc ggtgttgcag gctgcccatc gcggtggggt cccggtggct gaggtgatcg
     4741 catcgtcgtc tgctgcaacc g
//
LOCUS       DAORNF010000178         4760 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_13339_length_4760_cov_1.906270, whole genome shotgun sequence.
ACCESSION   DAORNF010000178 DAORNF010000000
VERSION     DAORNF010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4760
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13339_length_4760_cov_1.906270"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..514)
                     /locus_tag="PLV68_08310"
     CDS             complement(<1..514)
                     /locus_tag="PLV68_08310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01291.1"
                     /translation="MPGDDDDTVLAQASEPPQTTVHGRERHGWQRHAFAITVAAVVLV
                     LVLTGTTLSSMAAVGGGDGSDAGSGVVVGTPRELRTDEYLRTSPWRLGAIADPDLSFA
                     SPLSDDSALATTVTSHGVITRLVFFDSLIIELGHLTSTSMAFALLWWLPTLLLFACGP
                     TLLTRFGVPRV"
     gene            complement(554..721)
                     /locus_tag="PLV68_08315"
     CDS             complement(554..721)
                     /locus_tag="PLV68_08315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01292.1"
                     /translation="MLYEYQGRAVAYGAAAALMVVLIVTGMWLAGPQHLRRSSVTAGV
                     ETSSPAPQPTA"
     gene            complement(667..1809)
                     /locus_tag="PLV68_08320"
     CDS             complement(667..1809)
                     /locus_tag="PLV68_08320"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRE01293.1"
                     /translation="MSPATDSLTGDADSTPAHADSAPAQARALVWTFGLLTASLAAGY
                     GVMFAMLDDFRDAYGISEASLGTVVAVGFFSSFVAQVLFAPQADRGHARLLVFLGVAF
                     NLVGLIVMAYGSNLWLLLAGRVAMGVGTGMTLPAARRIVILADPDNLGHNIGRLLVAD
                     VFGFAIGPALSAVLVDPFGIAAPFLVIAVLTMVCVPVVARAHVDETTGDDQPTARFAF
                     DLLRIRPLAGAICFGMAAFLMIGTFDALWALVMDDLDTADWIANLGITLFSLPLIFLG
                     SFGGRLAQRVGPFRLATRCSWCSTARCRPEWRTSWCRSSTPSTTRSRSRPLASRWAWW
                     PPRNGWPARRACSVVPRPWSAVSRRSWPACSTSTRGAPSPTVPRRH"
     gene            complement(1806..>2183)
                     /locus_tag="PLV68_08325"
     CDS             complement(1806..>2183)
                     /locus_tag="PLV68_08325"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HRE01294.1"
                     /translation="RPDNGARRPTRALPDQVAAVNAIGDIGFAVFHGAVLIVVGTLLF
                     NIGSRTVPAVAITVLAQSEIAFAPVWVYIALGEAPGIRSLIGGSIIVGAVIGKALFDA
                     RPMRVLANQDLLEAPPQSGPAAV"
     assembly_gap    2184..2193
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2194..2832)
                     /locus_tag="PLV68_08330"
     CDS             complement(<2194..2832)
                     /locus_tag="PLV68_08330"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRE01295.1"
                     /translation="MTATSHTTLALDGRAAARHVPLGASVIAFSAGLVWSIGTLTARL
                     AKHSDAWQYLLWRSIGVVVVVEIYGYWSGKGSMIRRCFGGGRTMVIATLGLALSSLGY
                     VYAVKNTTAANAAFLSSITPLITVMFSRFFLGERLNKVTIVALSAAVCGLAITVSGDF
                     QSGNMAGNVSAVFCSIGFAIYTIALRSDPSRDWSPSMAGYAVVLIVVCGSVAA"
     gene            complement(3122..3409)
                     /locus_tag="PLV68_08335"
     CDS             complement(3122..3409)
                     /locus_tag="PLV68_08335"
                     /inference="COORDINATES: protein motif:HMM:NF017847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HRE01296.1"
                     /translation="MVRSAYHLLGLRTFLTTGEKETRAWTFHAGDKAPVCAGKIHTDI
                     QRGFIRAEVIHWDELLELGSWAKAKETGKLRIEGKDYEFLDGDVTEFRFNV"
     gene            complement(3354..4199)
                     /locus_tag="PLV68_08340"
     CDS             complement(3354..4199)
                     /locus_tag="PLV68_08340"
                     /inference="COORDINATES: protein motif:HMM:NF014036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HRE01297.1"
                     /translation="MERFGLVGLPNAGKSSLYNALTGGGALAAPYPFATKDPNIGVAK
                     VPDERVDRLGAMSKTKTLVHAAVQVVDIGGLVEGASKGEGLGNKFLANIREVEATVFV
                     LRAFEDGDVMGPSDPLEHLRIVEIELALADLETVEKRLRTTQRQAKLDKSIGSEIESL
                     EVAYASLSEGRPLYRANLKPEIVEALQPHFLLTNRPVLAVANVGENDIDSIPARPRRD
                     ERRRSQRRGDRHVRPTRSGGGRDRGPGRTGRDAGRVRPRRGRAVPHGPQRLSPARPAH
                     LPHHR"
     gene            complement(4246..>4760)
                     /locus_tag="PLV68_08345"
     CDS             complement(4246..>4760)
                     /locus_tag="PLV68_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01298.1"
                     /translation="IPEAMAPNLRNRGFTISAALNVPADGPCEGVIVGHGGHSGGYAL
                     YLQGGRLHYVNNLLGTQYTTVSAEVVLPPGEVLVSAEFVPTGRFQGEVQLRYGDVPVG
                     RGAIPLTTPLTYGVDPFAVGYQRMTSISPELTGRAEITPGVLHSVVIDPQGRAWRDPE
                     AESRAALATQ"
BASE COUNT          810 a         1616 c         1622 g          702 t
ORIGIN      
        1 ggacgcgcgg cacgccgaag cgggtcagca gcgtcggtcc acaggcgaac agcaacagcg
       61 tcggcagcca ccacaacagt gcgaacgcca tcgacgtgct ggtgaggtga ccgagctcga
      121 tgatcagtga gtcgaagaac accagccggg tgatcacgcc gtggctggtc accgttgtag
      181 cgagtgcgct gtcgtcactg agcggcgagg cgaacgagag gtcgggatcg gcgatcgccc
      241 ccagccgcca cgggctggtc cgcaggtact cgtcggtgcg gagttcacgc ggtgtgccga
      301 cgaccacgcc gctgcctgca tcactgccgt caccgcctcc gaccgcggcc atcgacgaca
      361 aggtggtgcc ggtcagcacc aacacgagaa cgaccgccgc gacggtgatc gcaaaggcgt
      421 gccgttgcca accgtgccgc tcgcgaccgt gcaccgtggt ttgtgggggc tcggaggcct
      481 gtgccaacac ggtgtcgtcg tcgtcgcctg gcatcggcgg cgacagtagt gccgcagggc
      541 ggcaaggagg cgactacgcc gttggttgtg gggccggact ggacgtttcc actccggcgg
      601 tgacgctgct gcggcggagg tgctgcggcc cggccagcca catcccggtg acgatcagca
      661 ccaccatcag tgccgccgcg gcaccgtagg cgacggcgcg cccctggtac tcgtagagca
      721 cgccggccat gatcgccgag acaccgccga ccagggtctg ggcaccaccg agcatgccct
      781 gcgcgccggc cagccgttcc ttgggggcca ccatgcccac cgcgacgcca gaggccgaga
      841 tcgtgaccgc gtcgttgacg gagtggacga gcgacaccac gaagttcgcc attccggtcg
      901 gcacctggcc gtagagcacc atgaacaccg cgtcgccagc cggaacggcc cgacccgctg
      961 ggcgagccgg ccaccgaagg agccgaggaa gatcagcggc agcgagaaca gggtgatacc
     1021 gaggttggcg atccagtcgg cggtatcgag gtcgtccatc accaatgccc acagcgcatc
     1081 gaaggtgccg atcatcaaga aggcggccat gccgaagcag atcgcgccgg cgagtggtcg
     1141 aatgcgtaac aggtcgaacg cgaaccgggc cgtgggttga tcgtcgccgg tggtctcgtc
     1201 gacgtgggcg cgggctacca ccggcacgca caccatcgtc agcaccgcga tgaccaggaa
     1261 cggcgcggcg atgccgaacg gatcgaccag cacggccgac agcgccgggc cgatcgcaaa
     1321 gccgaacacg tcggccacca acaggcgccc gatgttgtgg ccaaggttgt cagggtcggc
     1381 caggatgacg atgcgccgcg ccgcaggcag ggtcatgccg gtgccgaccc ccatcgcgac
     1441 ccgccccgcg agcagcagcc acaggttgct gccgtaggcc atcacgatca ggccgaccag
     1501 gttgaaggcg acgccgagaa acaccaacag ccgggcgtga ccccgatcgg cttgcggcgc
     1561 gaacagcacc tgggcgacga acgaactgaa gaagccgacg gcaaccacgg tgccgagcga
     1621 tgcttcgctg atgccgtacg cgtcgcggaa gtcgtcgagc atcgcgaaca tcacgccgta
     1681 gcccgcggcc aacgacgcgg tcagcagccc gaacgtccac accaaggccc gtgcctgagc
     1741 gggcgccgaa tcggcgtggg cgggggtcga atcggcgtcg ccggtgagtg aatcggtggc
     1801 gggactcaca cggccgccgg accggactgc ggcggtgctt cgagcagatc ctggttggcc
     1861 agcacgcgca tggggcgggc gtcgaacagg gccttgccga tcacggctcc gacgatgatc
     1921 gatccaccga tcagcgagcg gatgccgggg gcctcgccca gtgcgatgta cacccacacg
     1981 ggagcgaacg cgatctcgct ctgggccagc acggtgatcg ccaccgcggg aacggtgcgt
     2041 gagccgatgt tgaacagcag cgtgccgacc acgatcaaca cggcgccgtg aaagaccgcg
     2101 aagccgatgt cgccgatcgc gttcaccgcc gcgacctgat cgggcagcgc acgggtcggg
     2161 cgccgcgcgc cattgtctgg ccgnnnnnnn nnnagcggcc accgacccgc agaccacgat
     2221 cagcacgacc gcatatccgg ccatgctcgg cgaccagtca cggctggggt cgctgcgcag
     2281 ggcgatcgtg tagatcgcga agccgatcga gcagaacacg gcgctgacgt tgccggccat
     2341 gttgccgctc tggaagtcgc ccgacaccgt gattgccaag ccgcacaccg ccgcgctgag
     2401 ggcgacgatc gtgaccttgt tgagccgctc gccgaggaag aaccgggaga acatcacggt
     2461 gatcagcggc gtgatcgacg acaagaacgc ggcgttggcc gcggtggtgt tcttgaccgc
     2521 atacacgtaa cccagcgacg acagggccag gcccaaggtg gcgatcacca tcgtccgccc
     2581 gccgccgaag cagcgccgga tcatcgagcc cttgcccgac cagtagccgt agatctcgac
     2641 caccacgacg acaccgatcg agcgccacag caagtactgc cacgcgtcgc tgtgcttggc
     2701 caagcgggcc gtgagggtgc cgatgctcca gacgagtccg gccgagaacg cgatcaccga
     2761 cgcccccaac ggcacgtgtc gcgccgcggc tctgccgtcg agcgcgagcg tggtgtgtga
     2821 ggtggcggtc acaacggatc atcgtgccag acagcggccg aaaaatcgtg tccgattcga
     2881 tcgaagtccc tggtcacccc catcgcccac cccgatcacc cgcggctcga ccccaagcga
     2941 cgcctggtct cgtcgaagac agcgcgttcg tcggcggtca tggcggccac gcggtggttc
     3001 ttcagttcat catcgtcgaa gtggccatca gtcgtcctcc tcggcggtgt gtccttcggt
     3061 gctgcagcgg gtcattgccc aacgcgctcg cgtcacgcga gcgcagatcg cgaaaccctg
     3121 gctacacgtt gaagcggaat tcggtgacgt cgccgtcgag gaactcgtag tccttgcctt
     3181 cgatgcgcag cttgccggtc tctttggcct tggcccacga gccgagttcg agcagctcgt
     3241 cccagtggat gacctcggcg cggatgaagc cgcgctggat gtcggtgtgg atcttgcccg
     3301 cgcacacggg cgccttgtcg ccggcgtgga acgtccaggc ccgggtctcc ttctcaccgg
     3361 tggtgaggaa ggtgcgcagg ccgagcaggt gataggcgct gcggaccatg cggaacagcg
     3421 cgccctcgcc gaggccgaac ccttccagca tctcggcccg ttcggccggg tcctcgatcg
     3481 cggccgcctc cgcttcgagt tggacgcaca tgccgatcac ctcgacgttg gctccggcgt
     3541 cgttcatctc ggcgcggacg cgcggggatg ctgtcgatgt cgttctcgcc gacgttggcg
     3601 acagccagca ccgggcggtt ggtcagcagg aagtgaggct ggagtgcctc gacgatctcc
     3661 ggcttgagat tggcgcggta cagcggccga ccttcgctca gcgaggcgta ggccacctct
     3721 agcgactcga tctcggaccc gatcgacttg tcgagcttgg cttggcgctg ggtggtgcgc
     3781 agccgcttct cgacggtctc caggtcggcc aatgccagtt cgatctcgac gatacggagg
     3841 tgttcgagcg ggtcgctcgg ccccatcacg tcgccgtcct cgaaggcccg caacacgaac
     3901 accgttgcct cgacctcgcg gatgttggcc aggaacttgt tgccgagtcc ttcacccttg
     3961 ctcgctccct cgaccaggcc gccgatgtcg accacctgca ccgcggcgtg caccagggtc
     4021 ttggtcttgc tcatcgcgcc cagccgatcg acccgttcgt cgggcacctt ggcgacgccg
     4081 atgttggggt ctttggtggc gaacgggtac ggcgcggcca gcgcgccccc acccgtgagc
     4141 gcgttgtaca gcgagctctt gcccgcgttg gggaggccga ccagaccgaa gcgttccata
     4201 cggccccgag gctacgcccg cccggccgcg acggggcagg ccgcgctatt gcgtcgcgag
     4261 ggcggcgcgg gactcggctt cggggtcgcg ccaggcgcgg ccttgtgggt cgatcacgac
     4321 gctgtgcaac acccccggtg tgatctcggc tcggccggtg agctcggggc tgatcgaggt
     4381 catccgctgg tacccgaccg cgaacgggtc gacaccgtag gtgagcggcg tcgtcaacgg
     4441 gatcgcgccc cgtccgaccg gcacgtcgcc gtagcgcaac tgcacctcgc cctggaaccg
     4501 tccggtcggc acgaactcgg ccgacaccag cacctcgccg gggggcagca cgacttcggc
     4561 cgacaccgtg gtgtactgcg tgccgagcag gttgttgacg tagtgcagcc ggccgccctg
     4621 gagatacaac gcgtagccgc cgctgtggcc gccgtggccg acgatgacgc cctcgcacgg
     4681 cccatccgcg ggcacgttca gcgcggcgct gatcgtgaag ccacggttgc gcaggttggg
     4741 tgccatcgcc tcgggaatcg
//
LOCUS       DAORNF010000179         4755 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_13357_length_4755_cov_1.950213, whole genome shotgun sequence.
ACCESSION   DAORNF010000179 DAORNF010000000
VERSION     DAORNF010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4755
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13357_length_4755_cov_1.950213"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..569)
                     /locus_tag="PLV68_08350"
                     /pseudo
     CDS             complement(1..569)
                     /locus_tag="PLV68_08350"
                     /inference="COORDINATES: protein motif:HMM:NF023454.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3516 domain-containing protein"
     assembly_gap    606..639
                     /estimated_length=34
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <640..1065
                     /locus_tag="PLV68_08355"
     CDS             <640..1065
                     /locus_tag="PLV68_08355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01299.1"
                     /translation="RSVQADRATDVFALASARFVVVEQQTSGGLPRVGGAIEQVQQHR
                     RGHGEARFQALRWRLLQTRERGVVVVNVALRWRRLGGLALLLGVAAGLGQLVLILGLV
                     VGGEGDDESFGVVSGPPRAACDLVELACGCRCRRTCRAG"
     gene            complement(1147..2082)
                     /locus_tag="PLV68_08360"
     CDS             complement(1147..2082)
                     /locus_tag="PLV68_08360"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRE01300.1"
                     /translation="MSTLESYLTAAETPRSADGVIEAMVLWSAEHGRSLYPHQEEAIL
                     ELLSGNNVILATPTGSGKSTVALAAHAMSRVADTPEQRRRSWYTAPVKALVSEKFFDL
                     CQQLGPEHVGMMTGDSSINPDAAVMCCTTEILAFMALRDGALADADTVVLDEFHFYAD
                     PDRGWAWQVPLLEMSRATFLLLSATLGDTRTLAEDLTRRTGRATVTVDRSVRPVPLRY
                     AVSCCITWSRTCSPPTRHRCTSSTSPRPRQSSRRERWRRSSSSTPRPRPPSTRSWPAS
                     RCHQGSGATSPACCATASACTTPACCPATDAWSSG"
     gene            complement(2079..>2685)
                     /locus_tag="PLV68_08365"
     CDS             complement(2079..>2685)
                     /locus_tag="PLV68_08365"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01301.1"
                     /translation="HQSVNAAIALAAAGAFLQRAVNPMALRDGLAAVHPPARVECVEI
                     AVGRGRATVVFDRAHNPDAVAALTRTLRDDLPAAGQRALVLGVLAGRDLDTMVRALDV
                     ARYERIVVCSTPTVRGLDAERLAAAVQIHARCAVEVVPDPVTAAALVTGQARHGDQIV
                     VTGCTYHVAALREWLLGPVAAAHTATRTTTPTAHAATRSGR"
     assembly_gap    2686..2740
                     /estimated_length=55
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2741..3467)
                     /locus_tag="PLV68_08370"
     CDS             complement(<2741..3467)
                     /locus_tag="PLV68_08370"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HRE01302.1"
                     /translation="MTPRLATAIAWLEQHIDDAGWAARANRAGPIELSLTRMHALADA
                     LGNPQHRLRTVHITGTNGKGSTAAIVTRLLLAEGRRVGTYTSPHLSRYNERIQVDDTP
                     IGDDTFADLLERVAAAEAAIGQRSRPFELLTAAALCHFADQKVDVAVIEVGMLGRFDA
                     TNVVDADVAVITNIGFDHTTGEGDWRHRIVAEKAGIVTPRSVAILGPIEDRLAAVVAT
                     ERPRALWRFGRDITVTRDVAIPTG"
     gene            complement(3481..4419)
                     /locus_tag="PLV68_08375"
     CDS             complement(3481..4419)
                     /locus_tag="PLV68_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HRE01303.1"
                     /translation="MTATGHDAGPPTIPDTASPPGSFERTPGGAIVMNGNVLRDETVA
                     RLRREIDALGNPRVCLATVLVGDDRPSQVYVRSKHKKAEEAGMTSKHADLPATASQAQ
                     VEEAVLAFAADPSVHGILVQLPLPNGLDEEAVLDLVPADKDVDGLTEASMGRLMRGRP
                     GHVPCTPQGVLRLLDRYGVSTSGQRAVVIGRSSLVGLPLAVLLARKGIDATVTLAHSR
                     TTDLVAVCRDADIVIGAAGIAGLITAEHVKPGATVIDVGVSRTAAGIVGDVDFDAVQA
                     VAGAITPMPGGTGPMTIACLLENTLEAARLLGAVPR"
     gene            4493..>4755
                     /locus_tag="PLV68_08380"
     CDS             4493..>4755
                     /locus_tag="PLV68_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01304.1"
                     /translation="MSEPVTHLVGRDADLAHLGALLADPQVRLVTVIGQGGVGKTRLA
                     REAIAQSGRGADHFVELADIDDAALIPASIVRALGIETRPGQTV"
BASE COUNT          657 a         1563 c         1716 g          730 t
ORIGIN      
        1 cgtgccactc gtcgatcagg ctcgagtcgg tgacgcggat gacaccgtcg agccagtccg
       61 ccaactcgtg cacctcgtcg gacttgagcg cctcggggat ggtgcggtcg atcgtgcgca
      121 gggcgtcgct gaggtagcgc agcagcgtgc cctccgagcg cttcaggccg tggtggcgga
      181 cgaagccgtg gaagtcgagg gcgcgatccc acatctcgcg cacccttggg cgagatgttg
      241 gccccgtcga tccagcggtg ggtgagccgc cactcgtcga aggcctcgta cagctcgtcg
      301 gccagcggcg tgggccaggt gaccttggcg atccgctcga cccgttcttc gtagggcacg
      361 ccggcagcct tcatctcggc catcgcgtcg gtccgcgcct tgtcgcgctg ggccagcagg
      421 atctggcgtg ggtcgtcgag cacgcactcg accgcggcca gcacgtcgag cgccgatcgc
      481 tgcaccaacg cgggcaacac gtcgatcaag aagggcgcaa gcggctggtt gagcgcgaag
      541 tcctgttgca gcgagcggtc gaccaacacg cgacggccgt caggttgggg cacggcgagc
      601 tgctcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc gatcggtaca ggccgatcgc
      661 gcgacggacg tgtttgcgct ggcgagcgcg aggttcgtgg ttgtcgagca gcagacctcg
      721 ggcggcctgc cacgtgtcgg cggggcgatc gagcaggtgc agcaacatcg ccggggtcac
      781 ggtgaagctc gattccaggc gctccggtgg cgcttgttgc agacgagaga acgtggtgtc
      841 gtcgtagtga acgtagccct tcggtggcgg cggcttggtg gccttgcgct gcttcttggc
      901 gtcgccgctg gccttggcca gcttgttctg atactcggcc tcgtggtcgg gggcgagggc
      961 gacgacgagt ccttcggtgt cgtatccggc ccgccccgcg cggcctgcga tctggtggaa
     1021 ctcgcgtgcg gctgccgctg ccgtcgtact tgtagagccg ggtgaacagc accgtgcgga
     1081 tcggcagatt gacgccgacg ccgagggtgt cggtgcccga caccaccttg agcagaccgc
     1141 gctgggtcag ccgctcgacc aggcgtcggt ggcggggcag catgccggcg tggtgcacgc
     1201 cgacgccgtt gcgcagcagg cgggcgaggt cgcgcccgaa ccctggtggc atcgcgatgc
     1261 gggccagctc ctcgttgacg gcggccttgg ccgcggcgtc gagcttgacg atcgacgcca
     1321 gcgctcccgc ctgctcgact gcctcggcct gggtgaagtt gacgaggtac agcggtgccg
     1381 cgttggtggc gagcaggtcc tcgaccacgt gatgcaacag ctcacggcgt agcgcagcgg
     1441 caccgggcgc accgagcggt ccacggtgac cgtggcccgg ccagtgcgcc gggtgaggtc
     1501 ctcggccagc gtacgggtgt cgcccagcgt ggccgacagc agcaggaacg tggcccggct
     1561 catctccagc aacggcacct gccatgccca accgcgatcg ggatcggcgt agaagtggaa
     1621 ctcgtcgagc accaccgtgt cggcgtcggc cagcgccccg tcgcgcaggg ccatgaacgc
     1681 caggatctcg gtggtgcagc acatcaccgc ggcgtcgggg ttgatcgacg agtcgccggt
     1741 catcatgccg acgtgctcgg gtcccagttg ctggcacagg tcgaagaact tctcgctgac
     1801 caacgccttg acgggtgcgg tgtaccagct acggcgtcgc tgctcgggcg tgtcggccac
     1861 ccgcgacatg gcgtgcgccg cgagcgcgac ggtcgacttg ccggacccgg tgggcgtggc
     1921 gaggatgacg ttgttgccgc tgagcagttc gaggatcgcc tcttcctgat gggggtacag
     1981 cgaccggccg tgctcggccg accacagcac catcgcctcg atcaccccgt cggccgaacg
     2041 aggtgtctcg gcggccgtca ggtacgactc gagcgtgctc atcgccctga tcgcgtcgct
     2101 gcgtgtgccg tgggtgtcgt cgtgcgcgtc gcggtgtgag cagccgccac ggggccgagt
     2161 agccactcgc ggagcgcggc gacgtggtag gtgcagcccg tgaccacgat ctggtcgccg
     2221 tggcgggcct gcccggtgac gagcgcggcc gcagtcaccg ggtcgggaac cacttcgacg
     2281 gcgcagcgag catggatctg caccgctgcc gccaagcgct cggcatcgag accgcggacg
     2341 gtgggtgtgg aacagacgac gatccgctcg tagcgggcca cgtcgagggc acgcaccatc
     2401 gtgtcgagat cgcgcccggc gagcacgccc agcacgagtg cgcgctggcc cgcggccggc
     2461 agatcgtcac gcaaggtacg ggtcagtgcg gcgaccgcat cggggttgtg ggcgcgatcg
     2521 aacacgacgg tcgctcggcc ccgccccacg gcgatctcga cgcactcgac ccgcgccggt
     2581 ggatggaccg cagcgagacc gtcgcgcagc gccatcgggt tgacggctcg ctggaggaac
     2641 gccccggccg cggccagcgc gatcgcggcg ttgaccgact ggtgcnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ggccggtggg gatggcgacg
     2761 tcgcgcgtga ccgtgatgtc acggccgaag cgccacagcg cgcggggccg ttcggtcgcg
     2821 accacggccg cgaggcggtc ttcgatcggg ccgaggatcg ccaccgaccg aggggtgacg
     2881 atgccggcct tttcggccac gatgcggtga cgccagtcgc cttcgccggt ggtgtggtcg
     2941 aagccgatgt tggtgatgac cgcgacgtcc gcgtcgacca cgttggtggc gtcgaaacgg
     3001 ccgagcatgc cgacctcgat cacggcgaca tccaccttct gatcggcgaa gtgacacagc
     3061 gccgcggccg tcagcagttc gaaggggcgg gagcgctggc cgatcgcggc ttcggccgcc
     3121 gcgacgcgct ccagcaggtc ggcgaaggtg tcgtcaccga ttggcgtgtc gtcgacctgg
     3181 atccgctcgt tgtagcgcga caggtggggg ctggtgtagg tgccgacacg gcggccttcg
     3241 gccagcagca ggcgggtgac gatcgccgcc gtcgaacctt tgccgttggt gcccgtgatg
     3301 tgcaccgtgc gcaggcgatg ctgcgggttg ccgagcgcat cggcgagcgc gtgcatccgc
     3361 gtcaacgaca gctcgatcgg tccggcccgg ttggccctgg ccgcccaccc agcgtcgtcg
     3421 atgtgttgct ccagccacgc gatcgccgtc gccaggcgtg gggtcatggc agccacccct
     3481 tcagcgcggc accgctccga gcagacgggc ggcttcgagg gtgttttcga gcaggcaggc
     3541 aatggtcatc ggcccggtac cgcccggcat cggcgtgatc gcgccggcca cggcctgcac
     3601 cgcatcgaag tcgacgtcac cgacgatgcc cgccgcggtg cggctgacac cgacgtcgat
     3661 caccgtcgcg ccgggcttga cgtgttcggc cgtgatcagc ccggcgatgc cagcggcacc
     3721 gatcacgatg tcggcgtcgc ggcagaccgc gaccagatcg gtggtgcgcg agtgggcgag
     3781 ggtgaccgtg gcgtcgatgc ccttgcgggc cagcagcacc gcgagcggca gcccgacaag
     3841 cgacgagcgg ccgatgacca cggcccgctg accggaggtg ctgacgccgt agcggtcgag
     3901 cagacgcagc acgccctgcg gcgtgcacgg cacgtgtccg gggcggccgc gcatcaggcg
     3961 gcccatgctg gcctcggtca gcccgtcgac gtccttgtcg gccgggacca gatcgagcac
     4021 ggcctcttcg tcgagcccgt tcggcagcgg cagctgcacc aggatgccgt gcacgctggg
     4081 gtcggcggcg aacgcgagca ccgcctcttc gacctgggcc tggctggcgg tggcgggcag
     4141 atcggcgtgt ttgctggtca ttccagcctc ttcggccttc ttgtgcttgg agcgcacgta
     4201 cacctggctc ggccgatcgt cgccgaccag cacggtggcc aggcacaccc gcgggttgcc
     4261 gagcgcgtcg atctcgcggc gcaagcgagc caccgtctcg tcgcgcaaca cgttgccgtt
     4321 catgacgatg gccccgcccg gtgtccgctc gaacgatccg ggcggtgagg cagtgtctgg
     4381 gatggtgggt gggccagcat cgtggccagt cgcggtcatc ccggccaacc tacctgggcc
     4441 gcccgcgctg cccgcgagtc gctgcaaccg tgcctggcat catgggggct gcatgagtga
     4501 accggtgacg catctggtcg gccgtgacgc cgatctcgcg catctcggcg cgctgctggc
     4561 cgacccacag gtccggctgg tgaccgtcat cggacagggt ggcgtcggca agacacgtct
     4621 ggcccgggaa gccatcgccc agagtggtcg tggtgctgat cacttcgtcg agttggccga
     4681 catcgacgac gcggcgttga tccccgccag catcgtccgc gccctcggca tcgagacccg
     4741 cccggggcag accgt
//
LOCUS       DAORNF010000180         4751 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_13375_length_4751_cov_1.846465, whole genome shotgun sequence.
ACCESSION   DAORNF010000180 DAORNF010000000
VERSION     DAORNF010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4751
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13375_length_4751_cov_1.846465"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="PLV68_08385"
     CDS             <1..270
                     /locus_tag="PLV68_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HRE01305.1"
                     /translation="DDVRVIPVQNRFFGGNTAVTGLMVGEDLARVLDAEPASHRYLLP
                     DVCLSESRFLDGTTLDDLPRPVEVVPTNGIALRAALGLATGPTHA"
     gene            263..>794
                     /locus_tag="PLV68_08390"
     CDS             263..>794
                     /locus_tag="PLV68_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HRE01306.1"
                     /translation="MPDANQPPAPALPTVVIVGRPNVGKSTLFNRIVGEQVAIVEDRP
                     GITRDRKEVEAEWLGRSFLLVDTGGWLPRGSDLDAKVSRQVEAAVRGADLVLFVTDAS
                     VGVTDDDEAVAGWLRQIAPPVLLVANKADNDRREADRWEFLTLGIGDPVPVSALHGRR
                     AGDLLDEVIARIPDVSD"
     assembly_gap    795..898
                     /estimated_length=104
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <899..>1169
                     /locus_tag="PLV68_08395"
     CDS             <899..>1169
                     /locus_tag="PLV68_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HRE01307.1"
                     /translation="FNRLVGADRSVVHDMAGTTRDAIDTLVETEDGPIVFVDTAGMRR
                     RAKIDDSAEYYSLVRALRAIDDSDIALFVIDATAGVTGQDQRLAER"
     assembly_gap    1170..1270
                     /estimated_length=101
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1271..1619
                     /locus_tag="PLV68_08400"
     CDS             <1271..1619
                     /locus_tag="PLV68_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HRE01308.1"
                     /translation="LGFIGDAPILKISALSGKGVHKLRPVLQDAIEQYNRRVPTRDVN
                     KVIAAAQQRQPAPGGIKVLYAMQGATEPPTFTLFVNRELPATYLRYLERSIREAFDFG
                     STPIKLRVRRRSE"
     gene            1631..1906
                     /locus_tag="PLV68_08405"
     CDS             1631..1906
                     /locus_tag="PLV68_08405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01309.1"
                     /translation="MPWCEDCAKYHAPSAMRPDGTCPTCGRVLDAPKPERVTAKNLDL
                     KRLAAGHDADADPNDASVDDGSAPWHFKLLMVLLILYLGWRFYQLIF"
     gene            2243..>2564
                     /locus_tag="PLV68_08410"
     CDS             2243..>2564
                     /locus_tag="PLV68_08410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01310.1"
                     /translation="MPPTQIGAGFGGPPAGGEPPSGSSWLPQENPKKNNGALIGGLLA
                     AALLVIGAIYWFGVRSDAEDKAKDAQDRADEILKTIPTIDLDNITIPTIDFDNITIPQ
                     VPGIT"
     assembly_gap    2565..2741
                     /estimated_length=177
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2742..>2956
                     /locus_tag="PLV68_08415"
     CDS             <2742..>2956
                     /locus_tag="PLV68_08415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01311.1"
                     /translation="LFSGTQAGDVIAQVAAARGASPLRLLQAVIYPTYAFAQVQDPGI
                     PANVDEYSWRDGNLSAPAPVRLTGSGE"
     assembly_gap    2957..2966
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2967..3191
                     /locus_tag="PLV68_08420"
     CDS             <2967..3191
                     /locus_tag="PLV68_08420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01312.1"
                     /translation="NLFSDNEVNWAAIPGLREAALAQIPIEGAEVTHVIVQRNLPFTD
                     QVVIRVYVNGTRSSGYLEADAQGNVTKVQN"
     gene            3289..3365
                     /locus_tag="PLV68_08425"
     tRNA            3289..3365
                     /locus_tag="PLV68_08425"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3323..3325,aa:Pro,seq:ggg)
     assembly_gap    3514..3523
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3778..>4013
                     /locus_tag="PLV68_08430"
     CDS             3778..>4013
                     /locus_tag="PLV68_08430"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01313.1"
                     /translation="MCAIVLVAIVVVPLLDTVIASVRASSAAGSKANVETALRNAADR
                     VNRAPGSCNYTIYAQAAVQANGWDANRATVEHLH"
     assembly_gap    4014..4032
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4219..>4751
                     /locus_tag="PLV68_08435"
     CDS             4219..>4751
                     /locus_tag="PLV68_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01314.1"
                     /translation="MHRDTRHGGTRLLARSRGRRDRHKGDDAGVTLPELLVGIVMAAI
                     SSAIIVVVRQVDNTEGRLNNARSENQVSLWMPGDLASAAVDPSTNKLVNTDPATSPCF
                     GACPEGFEVIGSNALLLQWRVAGSLPDADGNVIYREQTRVSYRFALIGEEYVLWSSEE
                     FGFVRLDDRYSTGSSMLP"
BASE COUNT          723 a         1505 c         1409 g          693 t
ORIGIN      
        1 gacgacgtcc gcgtcattcc ggtgcagaac cgcttcttcg ggggcaacac ggccgtcacc
       61 ggtctgatgg tcggcgagga cctggcacgc gtgctggacg ccgaaccggc ttcgcaccga
      121 taccttcttc ccgacgtgtg tctcagcgag agccgctttc tcgacgggac aacactcgac
      181 gatctccctc gcccggtcga ggtggtgccc accaacggca tcgccttgcg cgcggcgttg
      241 ggccttgcca ctggacccac ccatgcctga cgccaaccag ccacctgctc ccgcgctgcc
      301 cacggtcgtc atcgtcgggc gccccaacgt cggcaagagc acgctgttca atcgcatcgt
      361 cggcgaacag gtcgccatcg tcgaagatcg ccccggcatc acccgcgatc gcaaagaggt
      421 cgaagccgag tggctcggcc gctcgttcct gctggtcgac accggtggct ggctgccgcg
      481 gggcagcgac ctcgacgcca aggtcagccg tcaagtcgaa gcggcggtgc gcggcgccga
      541 cctggtgctg ttcgtcaccg acgcgtcggt cggtgtcacc gacgacgacg aggccgtcgc
      601 cgggtggttg cgccagatcg ctccgcccgt gttgctggtt gccaacaagg ccgacaacga
      661 ccggcgcgag gccgatcggt gggagttcct caccctcggc atcggcgatc cggttcccgt
      721 cagcgcgctg cacggccgcc gcgcgggcga cctgctcgac gaggtgatcg cccgcatccc
      781 agacgtgtcc gaccnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngt
      901 tcaaccgcct cgtcggcgcc gaccgctcgg tggtgcacga catggccggt acgacgcgcg
      961 acgccatcga caccctggtc gagaccgagg acgggccgat cgtgttcgtc gacacggcag
     1021 gcatgcgccg cagggccaag atcgacgact cggccgagta ctactcgctg gtgcgggcct
     1081 tgcgggccat cgacgacagc gacatcgcgc tgttcgtcat cgatgccacg gcgggtgtca
     1141 ccggccagga ccaacgactg gccgaacggn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn gctggggttc atcggtgatg ccccgatcct caagatttcc gccttgagcg
     1321 gcaagggcgt gcacaagctg cgacccgtgc tgcaagacgc gatcgagcag tacaaccgcc
     1381 gtgtgcccac ccgcgacgtc aacaaggtga tcgctgcggc ccagcagcgc cagcccgcgc
     1441 cgggtggcat caaggtgctc tacgcgatgc agggcgccac cgagccgccg acgttcacgc
     1501 tgttcgtcaa ccgcgagctg ccggccacct acctgcgcta cctcgaacgg tcgatccgcg
     1561 aagccttcga cttcggctcg acgccgatca agttgcgcgt ccggcgccgg tccgaataga
     1621 gacgcacctg atgccctggt gtgaggactg cgcgaagtac cacgccccgt cggcgatgcg
     1681 tcccgacggc acgtgtccga catgcggccg ggtgctcgac gcgcccaagc ccgaacgggt
     1741 caccgccaag aacctcgacc tgaagcgcct tgccgcaggc cacgacgccg acgccgaccc
     1801 gaacgacgcc agcgtcgacg atggcagtgc cccgtggcac ttcaagctgt tgatggtgct
     1861 gctgatcctg tacctcggtt ggcgcttcta ccaactgatc ttctgaccga tctgccgggc
     1921 gttctgttgg ccgatctgct ggcctgatcc ggccacgggt gggacctgat caggactggt
     1981 tgcgtactgt cgattccccc cggcgatcgt gaccgtggtc gataatgctg tctccgtcat
     2041 tcgacgcgaa cagcctggcc ccacgaagcc aagtcccacg aaggagggca atcgtgaccg
     2101 tcccaccacc tcccggttcg ccataccccg gctcgccgtt cccatccccg ggccagcaac
     2161 cgccgactgg ccagtttccg ctcaggaccc cggcgcagca tggggtggtc cgccgaccca
     2221 ggccatgccg ccgacccagg ccatgccacc gacccagatt ggtgcgggct ttggcggccc
     2281 gcccgcaggt ggtgagccgc cgagtggttc gtcgtggctg ccccaggaga accccaagaa
     2341 gaacaacggt gcgttgatcg gcggtctgtt ggccgcggcg ctgttggtga tcggcgcgat
     2401 ctactggttc ggcgtgcgca gcgacgccga ggacaaggcc aaagacgccc aagaccgggc
     2461 cgacgagatc ctcaagacga ttccgacgat cgatctggac aacatcacga tccccacgat
     2521 cgatttcgac aacatcacca tcccgcaggt ccccggcatc acgannnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nacctgttca gcggcaccca
     2761 ggccggggat gtgatcgctc aggtcgcagc cgctcgcggt gcctcgccgc tgcgcctgct
     2821 gcaagcggtg atctacccga cctacgcctt cgcgcaggtg caggaccctg gtattccggc
     2881 caacgtcgac gagtacagct ggcgcgacgg caacctgtcg gctccggcac cggtgcgcct
     2941 gaccggcagc ggtgagnnnn nnnnnnaacc tgttcagcga caacgaggtc aactgggcgg
     3001 cgatcccggg tctgcgtgag gcagccttgg cccagattcc gatcgaaggc gccgaggtca
     3061 cccacgtgat cgtgcaacgc aatctgccct tcaccgatca ggtcgtgatc cgggtctacg
     3121 tcaacggcac ccgcagcagc ggctacctcg aagccgacgc ccaaggcaac gtcaccaaag
     3181 tccagaacta gtcgactgcg agcgtcagcc ggtggcgaga acccggctgt gcacgggagc
     3241 gggtttgcgg cgccgggatc ggcggatagc atcggcgctc cagtcactcg ggctgtagcg
     3301 cagcttggta gcgcacttgt ctgggggaca aggggtcgca ggttcaagtc ctgtcagccc
     3361 gaccagtaca caggcccagg tcacacgacc tgggcctgtg tcgttccctg gtgctgaccg
     3421 actgtgaccg tccgcggacg acagccggga gcggtcgggg agcggcgggc cgccacggtg
     3481 ggtggcgtcg tcgtcgattg tgtccagcga tcgnnnnnnn nnnagcatgg aaccagctac
     3541 caactgcccg ccctgggcac cgcctcgtgg gaaaacctga ttgccagccc atacgaacag
     3601 gacagcacga ttgtcgccgg cctggatgat ggccgcggcc atctggccgc cgctgatgcc
     3661 gcgcccggcg acatgggtgt cgtcgccgtc cggtcgtggc gcggccgggg tcgtcgacgt
     3721 ggtgccggcc cctgtcgggg tcgcagccga gatgagggct tcacgctggt cgagttggtg
     3781 tgcgcgatcg tcctggtcgc gatcgtcgtc gtaccgctgt tggacacggt gatcgcgtcg
     3841 gtccgcgcct cgtcggctgc gggtagcaag gccaatgtcg agaccgcgtt gcgcaacgcg
     3901 gccgaccgcg tcaaccgcgc tccgggcagc tgcaactaca cgatctacgc ccaagcggcg
     3961 gtgcaggcca acgggtggga cgccaaccgg gccaccgtcg aacacctgca ctannnnnnn
     4021 nnnnnnnnnn nncgacccag gccgggacct gggtcgctgg cgcatgcgaa ggcggcgtct
     4081 acaccgaaca gttggtgcaa ctcgtgcgga tcacgatcac cagccccaat ggccagtcat
     4141 ccggaacgat cgaagtgagc aagagcagtg tctgaacaca tcacgccgcc tcccgcccaa
     4201 cgccgaaccc gaccgcgggt gcaccgggat acacgccacg gtggtacacg tctgctggcg
     4261 cgctcccgcg gccggcgtga ccgtcacaag ggtgacgatg ccggcgtcac gctgcccgaa
     4321 ctgctggtcg ggatcgtgat ggctgcgatc tcgtcggcga tcatcgtcgt cgtccgccag
     4381 gtcgacaaca ccgagggtcg cctcaacaac gcccgttccg agaaccaggt cagcctgtgg
     4441 atgccgggtg atctcgcctc ggccgccgtc gaccccagta ccaacaagct ggtcaacacc
     4501 gacccggcca cctcaccctg cttcggtgcg tgccccgaag gcttcgaggt catcggatcg
     4561 aacgctttgc tgttgcagtg gcgggtcgcg ggcagcctgc cggatgccga cggcaacgtg
     4621 atctaccgcg agcagacgcg ggtgtcgtac cgcttcgccc tgatcggcga ggagtacgtg
     4681 ctgtggtcgt cggaggagtt cgggttcgtc cgactcgacg accgctactc gacgggctcg
     4741 tcgatgctgc c
//
LOCUS       DAORNF010000181         4751 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_13376_length_4751_cov_1.750426, whole genome shotgun sequence.
ACCESSION   DAORNF010000181 DAORNF010000000
VERSION     DAORNF010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4751
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13376_length_4751_cov_1.750426"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>370)
                     /locus_tag="PLV68_08440"
     CDS             complement(<1..>370)
                     /locus_tag="PLV68_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HRE01315.1"
                     /translation="TSRALGDDERALAGEHDAVNEQLRDLLLRIPNVPSADAPDGDGE
                     DDNPVVKGPINMPASFAEHQRVPHWETAAALGILDNERATKISGAMFTMQRGLGATMA
                     RALCQLALDDNADAFEEIRPP"
     assembly_gap    371..463
                     /estimated_length=93
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    719..933
                     /estimated_length=215
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            944..>1394
                     /locus_tag="PLV68_08445"
     CDS             944..>1394
                     /locus_tag="PLV68_08445"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRE01316.1"
                     /translation="MARVADTVRLYCDLYGAGVDPAALLTRVGLDERRTTNYRKLSGG
                     EKQRLSLAMALAARPAVAFLDEPTSGVDVGGRATIRTIVRELADDGCAVVLATHELDE
                     AERVADRVVIFNRGRVIADGSLDELRRGHDEIRFRSRPGLDTAALSAV"
     assembly_gap    1395..1447
                     /estimated_length=53
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1448..1643
                     /locus_tag="PLV68_08450"
     CDS             <1448..1643
                     /locus_tag="PLV68_08450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01317.1"
                     /translation="DEYAVSGGAALVAPLTVWLSEHGHPLADLRAGAQRLEDVFMRLT
                     GATTGDADRATGSLENGEQS"
     gene            1640..2386
                     /locus_tag="PLV68_08455"
     CDS             1640..2386
                     /locus_tag="PLV68_08455"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRE01318.1"
                     /translation="MSTAARRRSPLAAQARHEVTTTLRNGEQLLLTLIIPVLLLVFFS
                     LVDVLPADTPEPIDFLAPGILALAVMSAAMVSLGIGTGFERSYKVLKRLGTTPLGRSR
                     LVAAKIIAVVGVELVQFVVLIPIAYALGWNPGSPNWFAALGAVVLGTIAFAGLGLALA
                     GRLRGEINLAAQNGLYLVLLLLGGMVIPFGELPGSLSAIAKALPSGALADVLRDALAG
                     SGERPGASWLILTAWAAAMPSVAARTFRWE"
     gene            complement(2467..2802)
                     /locus_tag="PLV68_08460"
                     /pseudo
     CDS             complement(2467..2802)
                     /locus_tag="PLV68_08460"
                     /inference="COORDINATES: protein motif:HMM:NF021214.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
     assembly_gap    2803..2822
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2823..3229)
                     /locus_tag="PLV68_08465"
     CDS             complement(<2823..3229)
                     /locus_tag="PLV68_08465"
                     /inference="COORDINATES: protein motif:HMM:NF021214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HRE01319.1"
                     /translation="MIAVDYDANPWAFQAHPEVWLVVVFLVGAWLYAVRVIGPRVVAP
                     GEPVVTTRQRWYFIATIAMFWVASDWPIHDIGEGYLYSLHMLQHMMLTYFLPPLAILS
                     LPEWLLRIIVGDGRAYKVLRFFSKPVVAGVLFNL"
     gene            complement(3267..3593)
                     /locus_tag="PLV68_08470"
     CDS             complement(3267..3593)
                     /locus_tag="PLV68_08470"
                     /inference="COORDINATES: protein motif:HMM:NF015582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HRE01320.1"
                     /translation="MSLDIDYDDAPVKEHKHGLTDKGYVGIAVLLAVLTALEVMLTEI
                     DLPGGVFMALLLFLMVMKFLIVVSYFMHLKFDNKIFTYLFYSGLILALGVYIAALAAF
                     HFFSGG"
     gene            complement(3602..4351)
                     /locus_tag="PLV68_08475"
     CDS             complement(3602..4351)
                     /locus_tag="PLV68_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HRE01321.1"
                     /translation="MNHPPIPANPERSWLPLADTATPDAGTLAADVIDDGHDHDQHGH
                     DQHGHGDHGADHGDGHAHATSTGLSNNKMAMWVFLGSECLLFGGLISTYMLYRGRHEG
                     APTPNQLYNIPLTSVSSFVLLMSSLTMVLAVTAAQRKDERNTSVWLTVTALLGATFVG
                     AQVFEFTEFYKEGLGFTTNLFGSSFYTLTGFHGVHVTVGIIMLMSVVGMLKRSKIPGD
                     KAEVVELVGLYWHFVDIVWIIIFTLVYLIPA"
     assembly_gap    4383..4461
                     /estimated_length=79
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4462..>4751)
                     /locus_tag="PLV68_08480"
     CDS             complement(<4462..>4751)
                     /locus_tag="PLV68_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HRE01322.1"
                     /translation="ARKKEIAPLDPWDARTLEWMTASPPAEHNFDAVPTVHDTDEFFH
                     RKYQENDHGEVVQVATYEDIMAEEAKSADTHIHLPSPSYWPLVVAIGLPIVA"
BASE COUNT          736 a         1363 c         1468 g          724 t
ORIGIN      
        1 gggcgggcga atctcttcga acgcatcggc gttgtcgtcg agcgccaact ggcacagggc
       61 gcgggccatg gtggctccga gcccgcgctg catggtgaac atcgccccgc tgatcttggt
      121 ggcccgctcg ttgtcgagaa tgcccaacgc ggcggcggtc tcccagtgcg gcacgcgctg
      181 gtgctcggca aagctcgcgg gcatgttgat cggtcccttg acgaccgggt tgtcgtcctc
      241 accgtcaccg tcgggcgcat cggcgctggg cacgttggga atgcgcaaca gcaggtcacg
      301 cagctgctcg ttgaccgcat cgtgctcacc ggccaaggcc cgctcgtcgt ctcccagcgc
      361 gcgactggtg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncgtcgtg atgtcgcgca
      481 accgggcgtc gagttgggag acctgatcga gttggtccaa cagctcgggg ttccgacggc
      541 gcgccaaacc ggcgcgcaca ccgtcggggt cggtgcgaag caggcgaacg tcgatcacga
      601 tccgcaggct atccgggcac tcacgccagc cccgaggagg tttgcaccgc ggctacgacc
      661 ggtgggtagg ttcgcggtgt gacgtcagga tcgtcagcgg ccgtcgaggt cgaacaatnn
      721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnggtctca ccgatggctc gcgtcgccga
      961 caccgtgcgg ctgtactgcg acctctacgg cgcgggcgtc gatcccgccg cgctgttgac
     1021 acgggtgggg ctcgacgagc gccgcaccac caactaccgc aagctgtccg gcggcgagaa
     1081 gcagcggctg tcactggcga tggctctggc ggcacgcccg gcggttgcct tcttggacga
     1141 gccgacatca ggtgtcgacg tgggtgggcg agcgacgatc cgcacgatcg tgcgcgagct
     1201 cgccgacgac ggctgcgcgg tggtcctggc cacacacgag ttggacgagg ccgagcgcgt
     1261 cgccgaccgg gtggtcatct tcaaccgcgg ccgcgtgatc gccgacggtt cactggacga
     1321 gttgcgccga ggccacgacg agatcaggtt ccgctcacgg cccgggctcg acacagcggc
     1381 attgagcgcc gtacnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnncga cgagtacgcg gtcagcggtg gcgcggcatt ggtcgcgccg ctgaccgtat
     1501 ggctcagtga gcacggccat ccgttggccg acctgcgcgc gggggcgcag cggctggagg
     1561 acgtgttcat gcgcctgacc ggcgccacga ccggcgacgc cgatcgcgcc acgggcagcc
     1621 tcgagaacgg cgaacaatca tgagcacggc ggcgcgcagg cgcagcccgc tcgcagccca
     1681 agcccgccac gaggtcacca cgacgttgcg caacggcgag caactgctgt tgaccctgat
     1741 catccccgtg ctgctgctgg tgttcttctc gctggtcgac gtgttgcctg cagacacacc
     1801 cgagccgatc gacttcctcg cgcccggcat cctcgccctg gcggtgatgt cggcggcgat
     1861 ggtcagcttg ggcatcggca ccggcttcga gcgcagctac aaggtgctca agcgtctcgg
     1921 caccaccccc cttggccgca gccgactcgt cgcggccaag atcatcgcgg tcgtcggcgt
     1981 cgagctggtc cagttcgtgg tgctgatccc catcgcctac gccctcggct ggaaccccgg
     2041 ttcgcccaac tggttcgctg cgctgggcgc cgtggtgctg ggcacgatcg cgttcgccgg
     2101 cctaggcctg gccttggcgg ggcgcttgcg gggcgagatc aacctcgccg cccagaacgg
     2161 gctctacttg gtgctgttgt tgttgggcgg gatggtgatc ccgttcggcg agttgcccgg
     2221 ctctctgtcg gcgatcgcca aagcactgcc gtcgggggcg ctggccgacg tgctgcgcga
     2281 tgcactcgcc ggatcaggcg agcgccccgg ggcatcgtgg ctgatcctga ccgcctgggc
     2341 agcggcgatg ccgtcggtgg cagcacgcac gttccgctgg gagtgatcgg ctggtaggcg
     2401 tggcttgccg gtgtgccacc gcggcgacgg cgtgccctcg gccacggcaa agcgcttgaa
     2461 gaacatgtag atgatgatcg cccacaggaa gatggtgccg cccgtcttca tgatgccgcc
     2521 tgcgatctgc tgatcgttga tgacgtcgac gccccacacc cgcaccggga tgtcgtagtg
     2581 cttgtagacc gggcgctcgg cgaaggtcaa ccatccggcc ggcaccgtgg gcaccacgct
     2641 ctgggcgaac aggtacacca tcttgccgcc gtagcccatc tgcagttcct tgaacggccc
     2701 gatgatcggc atccacatca acagcgcggt ggtcaccaac agcacgtgca acaagtagtg
     2761 cagcaccgcg ttctcggtgg acgcattgac cagcccgggc acnnnnnnnn nnnnnnnnnn
     2821 nnaggttgaa caacaccccg gccacgacgg gcttggagaa gaagcggagc accttgtagg
     2881 cccggccgtc gccgacgatg atccgcaaca gccactcggg cagcgacagg atcgccagcg
     2941 gtggcaggaa gtaggtgagc atcatgtgct gcaacatgtg cagcgagtac agatacccct
     3001 cgccgatgtc gtggatgggc caatcgctgg ccacccagaa catggcgatg gtggcgatga
     3061 agtaccaccg ctgccgcgtg gtgaccaccg gttccccggg ggccacgacg cgcgggccga
     3121 tgacccgcac ggcgtagagc cacgctccca ccaagaagac gacgacgagc cagacctctg
     3181 ggtgcgcttg aaatgcccac gggttggcgt cgtagtcgac agcgatcatc acgtcgatca
     3241 ggcacccggg gaatcaggca cggcgatcag ccgcccgaga agaagtggaa ggctgcaagc
     3301 gcggcgatgt agacgccgag cgccaagatc aagcccgaat agaacagata cgtgaagatc
     3361 ttgttgtcga acttgaggtg catgaagtag ctgacgacga tgaggaactt catcaccatc
     3421 aggaacagca gcagcgccat gaacacgcca ccgggcaaat cgatctcggt cagcatcacc
     3481 tcgagcgcgg tgagcaccgc gagcagcacg gcgatgccga cgtaaccctt gtcggtcaga
     3541 ccgtgcttgt gctctttgac cggagcgtcg tcgtagtcga tgtcaaggct catgatctac
     3601 ctcacgccgg gatcaagtag acgagggtga agatgatgat ccacacgatg tcgacgaagt
     3661 gccagtacag accgaccaac tcgacgactt cggctttgtc gccggggatc ttgctgcgct
     3721 tcagcatgcc gaccaccgac atcagcatga tgatgccgac ggtgacgtgg acgccatgga
     3781 agccggtgag cgtgtagaag ctcgacccga acaggttggt ggtgaacccg aggccttctt
     3841 tgtagaactc ggtgaactcg aacacctggg ccccgacgaa ggtggctccc aacaaggcgg
     3901 tgacggtgag ccacacgctg gtgttgcgct cgtccttgcg ctgcgcggcc gtgacggcca
     3961 gcaccatggt gagcgagctc atcagcagga cgaacgagct gaccgaggtg agcgggatgt
     4021 tgtacagctg gttcggcgtc ggcgcaccct cgtggcggcc gcggtacagc atgtaggtgc
     4081 tgatgaggcc accgaacagc aagcactcgc tgcccaggaa gacccacatc gccatcttgt
     4141 tgttcgacag cccggtgctg gtggcgtggg catggccatc accatgatca gcgccgtggt
     4201 cgccgtgacc gtgctgatcg tgaccgtgct gatcgtgatc gtggccgtcg tcgatgacgt
     4261 cggcggcgag cgtgccggcg tcgggagttg cggtgtcagc caagggaagc caactccttt
     4321 ccgggttggc cggaatcggc gggtggttca tagtcggact cgggggcgac cgagggctcg
     4381 atnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4441 nnnnnnnnnn nnnnnnnnnn nggcgacgat cggcaacccg atggcgacca ccagcggcca
     4501 gtacgacggc gatggcaggt gaatgtgcgt gtcggccgac ttcgcctctt cggccatgat
     4561 gtcctcgtag gtggcgacct ggacgacctc gccgtggtcg ttctcctggt acttgcggtg
     4621 gaagaactcg tcggtgtcgt gcaccgtcgg gaccgcgtcg aagttgtgct cggcgggcgg
     4681 gctggccgtc atccactcga gggtgcgggc atcccacgga tcgagcggcg caatctcctt
     4741 cttgcgggcg g
//
LOCUS       DAORNF010000182         4743 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_13412_length_4743_cov_1.734002, whole genome shotgun sequence.
ACCESSION   DAORNF010000182 DAORNF010000000
VERSION     DAORNF010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4743
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13412_length_4743_cov_1.734002"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>310)
                     /locus_tag="PLV68_08485"
     CDS             complement(<1..>310)
                     /locus_tag="PLV68_08485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01323.1"
                     /translation="NEYAELVQWRGTARGLQRLLELISMEPAEVRDSGGVFVEGELEP
                     PPAHVKLSVRSSGWATDNDVLRIVRNELPAHVTFELHVGSRRIWPPEPDRGGGSDVPI
                     L"
     assembly_gap    311..477
                     /estimated_length=167
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(559..>821)
                     /locus_tag="PLV68_08490"
     CDS             complement(559..>821)
                     /locus_tag="PLV68_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative baseplate assembly protein"
                     /protein_id="HRE01324.1"
                     /translation="ALAALYRYINPISGGADGNGWAFDTDLNAAAIFQLLESVEGVER
                     VDEVLFFEYDLRNHERIGFGKDVVKLDRDSLFLSANHQVVVR"
     assembly_gap    822..880
                     /estimated_length=59
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<881..>1228)
                     /locus_tag="PLV68_08495"
     CDS             complement(<881..>1228)
                     /locus_tag="PLV68_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HRE01325.1"
                     /translation="LAAAIGGVDAETVANAKLRGPQTLRSGARAVTAHDFERIAAEAD
                     PSIARVRCLPPLDTGRPIRLLLVPTVTRRPEQIQLDDFALPDEMTNRVRERLDERRIL
                     GTTIEIGTPYYQGV"
     assembly_gap    1229..1336
                     /estimated_length=108
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1337..>1639)
                     /locus_tag="PLV68_08500"
     CDS             complement(<1337..>1639)
                     /locus_tag="PLV68_08500"
                     /inference="COORDINATES: protein motif:HMM:TIGR02243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative baseplate assembly protein"
                     /protein_id="HRE01326.1"
                     /translation="REVLGQSNGKPDQVFTVGATPVLARRLDETVTVSRDGITEDWTE
                     VPDFGRSGPTDKHFVWNSTTGEVRFGPLIRYPDGSTRQHGAAPREGAMIAVTGYRS"
     assembly_gap    1640..1728
                     /estimated_length=89
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1729..2528)
                     /locus_tag="PLV68_08505"
     CDS             complement(<1729..2528)
                     /locus_tag="PLV68_08505"
                     /inference="COORDINATES: protein motif:HMM:TIGR02243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative baseplate assembly protein"
                     /protein_id="HRE01327.1"
                     /translation="MTLPVTNLDDRNFQDIVDEAKRLIPTFCPEWTNHNLSDPGVALI
                     ELFAWMTEMSLFRLNQVPDVFYTRMLNLVGFEPFPATAARADITFWLTTALDEPVVVP
                     AETEVATAGDIGETRVFSTLDDLVIAPPRVIAALTSIGADTYADVWDDLRIDQGAVAC
                     FRTVTPGDAFYLGFENTLAGVALRLQIDANVEGIGVLPDRPPMRWEVWQGEGWIPVRS
                     YRDSTGGLNRNGNVVLLVPGQHEPLTLGGRRAFWLRARLLVAEPGQPT"
     gene            complement(2525..2938)
                     /locus_tag="PLV68_08510"
     CDS             complement(2525..2938)
                     /locus_tag="PLV68_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HRE01328.1"
                     /translation="MSDSAADFIGRGISFPMRVDQSGSLALTSGADDIDASLRMVLIT
                     APGERLMRPKFGCRIWDLLFEPVNANTIGLMAEAVREAVSQWEPRVILDEVDIDADPN
                     DHSRVMINLRYRVRLTNDKRNLVYPFYVIPQEGES"
     gene            complement(2986..>4442)
                     /locus_tag="PLV68_08515"
     CDS             complement(2986..>4442)
                     /locus_tag="PLV68_08515"
                     /inference="COORDINATES: protein motif:HMM:NF033848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HRE01329.1"
                     /translation="HSRVRTFTNSKYSDMVSKVATENGLQGQVTATTQSHEYLLHTGT
                     DRALLDMIAIRTGMEWFVDDSKLVFREPESHAGTQEAILGINLIKFRARYSGASHVDK
                     VTVRGWDYKAKDALVSVDSSSSADPDGASNPAKLGDYRRASTSVTGRDPEITTAALGV
                     ETTEEAQLIAKSIAERNAAHELSARGVMLGNPNVRAGALLKVGRVGTALSGTYYLTEV
                     EHVFGNGDQITRFSAGGPSGDALVDLVNGTTGAIGAWAAHGFVIGVVTNIKDPDGLNR
                     IKVKYPALSGDDESGWARLVQPMAGNEAGWVSVPAINDEVLVGFEHGDFRRPVVLGGL
                     YNGRDAPPYPVQDGKVTKHGFKTLSQHELVFVEEQGSESITIQHGTAPGTKVVLEKEK
                     VQVLVEGDKMVEIKAGMATMVLNEGEVTIKGKKVTIEATSGDLTLKGMNIKATAQQNF
                     EAAANIGATVKGNATAELSASGMTTVKGATVAIN"
     assembly_gap    4443..4452
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4453..>4743)
                     /locus_tag="PLV68_08520"
     CDS             complement(<4453..>4743)
                     /locus_tag="PLV68_08520"
                     /inference="COORDINATES: protein motif:HMM:NF033848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01330.1"
                     /translation="IDGSALSPEHEASLVEARVLQSVGSASRCELRFFDHDFRLIDGN
                     VLTIGKSVTVGFDTPRAQTVQVFAGEIVAIGVEQAGETRHQLVVEAFDKSHRL"
BASE COUNT          677 a         1441 c         1430 g          762 t
ORIGIN      
        1 gaggatcggg acatcgctac cgccaccgcg gtcgggttct ggcggccaga tgcggcgcga
       61 cccgacgtgc agctcgaacg tgacatgggc gggcaactcg ttgcggacga tgcgcaacac
      121 gtcgttgtcg gtggcccaac ccgacgaccg gaccgacagc ttgacgtggg cgggcggcgg
      181 ttcgagctcg ccttcgacga acacgccacc gctgtccctg acctcggccg gctccatgct
      241 gatcagttcc aacaggcgct gcaaaccgcg ggcggtgcca cgccactgca ccagttctgc
      301 gtactcgttg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngga
      481 tgatgcccaa gtagcgctgc aggaactcgt cctcggccat gcccaccggc agctgatgca
      541 acaaccagtc gtcgcgcctc atcgcacgac cacctggtgg ttggccgaca ggaacagcga
      601 gtcacggtcg agcttgacca cgtctttgcc gaagccgatc cgctcgtgat tgcggaggtc
      661 gtactcgaag aacagcacct cgtcgacgcg ctcgacgcct tcgaccgact ccagcaactg
      721 gaagatcgcg gcagcgttga ggtcggtgtc gaatgcccac ccgttgccgt cggccccgcc
      781 gctgatcggg ttgatgtatc ggtacagcgc cgccaacgca cnnnnnnnnn nnnnnnnnnn
      841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn acaccttggt agtagggcgt
      901 accgatctcg atcgtggtgc ccaggatgcg ccgctcgtcg aggcgctcgc gcacccggtt
      961 ggtcatctcg tcgggcaggg cgaagtcgtc gagctggatc tgctccggcc gccgggtcac
     1021 ggtcggcacc aacagcagcc ggatcggtcg gcccgtgtcg agcggcggca agcagcgcac
     1081 ccgggcgatc gacggatcag cctcggccgc gatgcgctcg aagtcgtggg cggtgaccgc
     1141 gcgggcgccg ctgcgcaacg tctgcgggcc gcgcagcttg gcgttggcga ccgtctcggc
     1201 atcgacaccg ccgatggccg cggcgaggnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnncgct gcggtacccg gtgaccgcga tcatcgctcc ttcgcgcggc
     1381 gcagcaccgt gctgacgcgt gctgccgtcc gggtagcgga tcaacggccc gaagcggacc
     1441 tcgcccgtgg tggagttcca cacgaagtgc ttgtcggtcg ggccgctgcg accgaagtcg
     1501 gggacctcgg tccagtcttc ggtgatgccg tcgcgcgaca cggtgacggt ctcgtcgaga
     1561 cggcgtgcga gcacgggcgt tgcccccacc gtgaacacct ggtcaggctt gccgttgctc
     1621 tgcccgagca cctcgcgctn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnta ggtgggctgg
     1741 cctggctcgg ccaccagcag gcgcgcccgc agccagaacg cacggcggcc gcccagcgtg
     1801 agcggttcgt gctgaccggg caccagcaac acgacgttgc cgttgcggtt gaggccaccg
     1861 gtggagtcgc ggtacgagcg caccggaatc cagccttcgc cctgccacac ctcccagcgc
     1921 atcggcgggc ggtcgggcaa cacgccgatg ccctcgacgt tggcatcgat ctgcaagcgc
     1981 agcgccacac cggccagcgt gttctcgaac cccaggtaga acgcgtcgcc gggggtcacc
     2041 gtgcggaagc aggcgacggc accctggtcg atgcgcaggt cgtcccacac gtcggcgtag
     2101 gtgtcggcac cgatcgaggt cagcgcggcg atgacgcgcg gcggcgcgat gaccagatcg
     2161 tcgagcgtgg agaacacacg ggtctcgccg atgtcgccag cggtggcaac ctcggtctcg
     2221 gccggcacca cgaccggttc gtcgagtgcg gtggtgagcc agaacgtgat gtcggcccgc
     2281 gccgccgtgg cgggaaaggg ttcgaatccg accaggttga gcatgcgcgt gtagaacacg
     2341 tcgggcacct ggttgaggcg gaacagcgac atctccgtca tccaggcgaa gagctcgatc
     2401 agcgccacgc ccggatcgga caggttgtgg ttggtccact cggggcagaa cgtggggatc
     2461 agccgtttgg cttcgtcgac gatgtcctgg aagttgcggt cgtcgaggtt ggtgaccggc
     2521 agcgtcatga ctctccctcc tggggaatga catagaaggg atagaccagg ttgcgcttgt
     2581 cgttggtcag gcgcacccgg tagcgcaggt tgatcatcac ccgcgagtgg tcgttgggat
     2641 cggcgtcgat gtcgacctcg tcgaggatga cgcgcggctc ccactgcgac acggcctcgc
     2701 gcaccgcttc ggccatcaac ccgatcgtgt tggcgttgac gggttcgaac aacagatccc
     2761 agatacggca gccgaacttg ggccgcatca aacgctcacc cggggcggtg atcagcacca
     2821 tccgcagcga cgcgtcgatg tcgtcggcac cggacgtgag cgcgagcgag cccgactgat
     2881 cgacgcgcat cggaaacgag atgccccgtc cgatgaagtc tgctgctgaa tcactcatcg
     2941 ctgtgtcccg tatggtccca tgtcgcccac tgctgctccg tccgatcagt tgatcgcgac
     3001 ggtcgcgccc ttcaccgtgg tcatacccga cgccgacagt tcggccgtgg cgttgccctt
     3061 caccgtcgcc ccaatgttcg ccgccgcctc gaagttctgc tgcgccgtgg ccttgatgtt
     3121 catgcccttc agcgtgagat caccggaggt tgcttcgatg gtgaccttct tgcccttgat
     3181 ggtgacctcg ccctcgttga gcaccatcgt cgccatgccg gccttgatct cgaccatctt
     3241 gtcgccctcg accaggacct ggaccttctc cttttcgagc accaccttgg tgccaggggc
     3301 cgtgccgtgc tgaatggtga tgctctcgct gccctgctct tcgacgaaca cgagctcgtg
     3361 ctggctcagc gtcttgaacc cgtgcttggt gaccttgccg tcctgcactg gatacggcgg
     3421 cgcatcccgg ccgttgtaca gcccgcccag cacgaccggg cggcggaagt cgccgtgttc
     3481 gaagccgacc agtacctcgt cgttgatggc gggcaccgac acccacccgg cctcgttgcc
     3541 agccatcggc tgcaccaggc gggcccagcc gctctcgtcg tctcccgaca gcgcggggta
     3601 tttgaccttg atccggttga ggccgtcggg gtccttgatg ttggtgacca ccccgatgac
     3661 gaagccgtgg gcggcccacg cgccgatcgc gccggtggtg ccgttgacca ggtcgaccaa
     3721 cgcatcgccg ctggggcccc cggcgctgaa gcgggtgatc tgatcgccgt ttccgaacac
     3781 gtgctcgacc tcggtgaggt agtacgtgcc gctcaacgca gtgcccacgc ggccgacctt
     3841 gagcagcgca ccggcgcgca cgttggggtt gcccagcatg acgccgcggg cactgagctc
     3901 gtgggccgcg ttgcgctcgg cgatcgactt ggcgatcagc tgggcctctt cggtggtctc
     3961 gacaccgagt gccgcggtcg tgatctcagg atcgcgacca gtcaccgaag tgctcgcccg
     4021 gcgatagtcg ccgagtttgg ccgggttgga tgcgccatca gggtcggccg agctggacga
     4081 gtcgaccgaa acgagtgcgt ccttggcctt gtagtcccat ccgcgcacgg tcaccttgtc
     4141 gacgtgactt gcgccgctgt agcgggcccg gaacttgatc aggttgatgc cgaggatggc
     4201 ctcctgggtg ccagcatgcg actcgggctc gcggaacacc aacttgctgt cgtcgacgaa
     4261 ccactccatg ccggtgcgga tcgcgatcat gtcgagcaag gcgcgatcgg tgccggtgtg
     4321 cagcagatat tcgtggctct gcgtcgtggc agtgacctgg ccctgcagcc cgttctcggt
     4381 ggccaccttg gacaccatgt cgctgtactt cgagttggtg aaggtacgca cacgcgagtg
     4441 gtnnnnnnnn nncagccggt gcgacttgtc gaacgcttcg accaccagtt ggtgccgggt
     4501 ctcgccagcc tgctccacgc cgatcgcgac gatctcaccg gcgaacacct gcaccgtctg
     4561 tgccctcggc gtatcgaagc cgacggtgac gctcttgccg atcgtcaaga cgttgccgtc
     4621 gatcagcctg aagtcgtgat cgaagaagcg caactcgcag cggctggccg agccgaccga
     4681 ctgcaacacc cgtgcctcga ccaggctggc ctcgtgctcg ggcgacaggg ccgagccgtc
     4741 gat
//
LOCUS       DAORNF010000183         4737 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_13434_length_4737_cov_2.067065, whole genome shotgun sequence.
ACCESSION   DAORNF010000183 DAORNF010000000
VERSION     DAORNF010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4737
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13434_length_4737_cov_2.067065"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="PLV68_08525"
     CDS             complement(<1..248)
                     /locus_tag="PLV68_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HRE01331.1"
                     /translation="MSDNAKLRRNARIRRHNRVRKKVHGTAERPRLAVFRSNKHIVLQ
                     VIDDEAGTTLAAASTNEASAREQGKGGNVAAAARMGALV"
     gene            complement(263..805)
                     /gene="rplF"
                     /locus_tag="PLV68_08530"
     CDS             complement(263..805)
                     /gene="rplF"
                     /locus_tag="PLV68_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008372550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HRE01332.1"
                     /translation="MSRIGKAPITVPSGVDVAIDAGTVSVKGPKGSLSFAVPGAITVR
                     ADANTLLVERPNDERDNRSLHGLTRTLVSNMVVGVTDGFSKELEIIGVGYRAEAQSPT
                     KLRLALGFSHPVLIDAPEGITFEVPTPTRVIVRGIDKEVVGQVAANIRSVRKPEPYKG
                     KGVRYLGERVLRKAGKTGKK"
     gene            complement(819..1220)
                     /gene="rpsH"
                     /locus_tag="PLV68_08535"
     CDS             complement(819..1220)
                     /gene="rpsH"
                     /locus_tag="PLV68_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HRE01333.1"
                     /translation="MLTDPIADMLTRIRNANTAMHDEVPMPSSKQKVALAKILQQEGF
                     IDGYSVAPAPKGPGQVLTVKMKYTPERARTIMGIKRVSTPGLRVYRKAAEVPRVLGGL
                     GVAVLSTSSGLLSDREARKRNVGGEILCYVW"
     gene            complement(1227..1412)
                     /locus_tag="PLV68_08540"
     CDS             complement(1227..1412)
                     /locus_tag="PLV68_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003948630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HRE01334.1"
                     /translation="MAKKSKINKAAATPKFKVRGYTRCRRCGRARSVYRKFGLCRVCL
                     REMAHAGEVPGLTKASW"
     gene            complement(1424..>1678)
                     /locus_tag="PLV68_08545"
     CDS             complement(1424..>1678)
                     /locus_tag="PLV68_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01335.1"
                     /translation="YALGLKEQIVFPEIDYDKIDAPHGMDITIVTTATTDAAGRALLD
                     AFGFPFKRGADAAAAPRKVKRRGPVRGGKGAAQPKAKKKK"
     assembly_gap    1679..1736
                     /estimated_length=58
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1737..2069)
                     /gene="rplE"
                     /locus_tag="PLV68_08550"
     CDS             complement(<1737..2069)
                     /gene="rplE"
                     /locus_tag="PLV68_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016637787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HRE01336.1"
                     /translation="MSDTAIATVPRLKQKYQDEVKAALKEQLGIANVMQIPKFEKIVI
                     NMGVGRATQQPSLLEGAVSDLQKITGQKPIVTKSKKSIASFKLREGQLIGAKVTLRQD
                     RMYEFLDRL"
     gene            complement(2069..2371)
                     /gene="rplX"
                     /locus_tag="PLV68_08555"
     CDS             complement(2069..2371)
                     /gene="rplX"
                     /locus_tag="PLV68_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HRE01337.1"
                     /translation="MRIKKGDHVVVISGKDKGREGEVIKALPKLDKVVVSGVNTATKH
                     QKATSQNQQAGIIEVDMPIHVSNVMLVHKGKPTRVGYKIQDDGAKVRVAKRTGEVI"
     gene            complement(2371..>2695)
                     /gene="rplN"
                     /locus_tag="PLV68_08560"
     CDS             complement(2371..>2695)
                     /gene="rplN"
                     /locus_tag="PLV68_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HRE01338.1"
                     /translation="GKEVLCIKVLGGTRRRYAGIGDIFVATVKDAIPGAGVKKGDVVK
                     CVVVRTKKERRRPDGSYIRFDENAAVIIKDDLTPRGTRIFGPVGRELRDKKFMRIVSL
                     APEVL"
     assembly_gap    2696..2725
                     /estimated_length=30
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2737..2988)
                     /gene="rpsQ"
                     /locus_tag="PLV68_08565"
     CDS             complement(2737..2988)
                     /gene="rpsQ"
                     /locus_tag="PLV68_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006304487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HRE01339.1"
                     /translation="MRNRRKVREGVVVSNKMDKTAVIAVVERVRHPKYGKFMMQTKKL
                     YAHDETNDVNIGDKVRVMETRPLSKLKRWRVFEIVERAK"
     gene            complement(3162..3392)
                     /gene="rpmC"
                     /locus_tag="PLV68_08570"
     CDS             complement(3162..3392)
                     /gene="rpmC"
                     /locus_tag="PLV68_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HRE01340.1"
                     /translation="MAKRSKKSELAELDLAGLINELRATKQEALNLRFRHATGQLENT
                     AALGNTRRQIARINTLIRQKEIAAAEAVQSAK"
     gene            complement(3394..3810)
                     /gene="rplP"
                     /locus_tag="PLV68_08575"
     CDS             complement(3394..3810)
                     /gene="rplP"
                     /locus_tag="PLV68_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HRE01341.1"
                     /translation="MLMPRKVKHRKQHRGRTAGLTKGGATLTQGEYGIQALEPGWLTA
                     RQIEAARIAMTRAIKRGGKVWINVFPDKPVTKKPAETRMGSGKGNPEYWVAVVRPGRV
                     LFELSFPNEEQAKAALNKAVQKLPIKARVITREEVI"
     gene            complement(3817..>4737)
                     /gene="rpsC"
                     /locus_tag="PLV68_08580"
     CDS             complement(3817..>4737)
                     /gene="rpsC"
                     /locus_tag="PLV68_08580"
                     /inference="COORDINATES: protein motif:HMM:TIGR01009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HRE01342.1"
                     /translation="PKHPTTAPPTLRKATDMGQKINPYGFRLGITTDWKSRWFSERDY
                     KEYLTEDWKIRRSIMTRLESAAISRIEIERTRDKLRVDIHTARPGIVIGRRGAQADEL
                     RAMLTKITGNPKVQLNIVEIKSPELDAALIAQGVADQLINRIAFRRAMKRAVQNAQKA
                     GALGIRVQCSGRLGGAEMSRTEWYREGRVPLHTLRADIDYGFREAKTTSGRVGVKVWI
                     YKGDILPYKPQNDDKILREAAMAVGETSGAPRKVVSSGARRPGADDAPEAAPLLKEAD
                     PEFEKLLDEEEQIARRTAAGHEAPHFRGED"
BASE COUNT          650 a         1468 c         1581 g          950 t
ORIGIN      
        1 accagcgcac ccatgcgcgc tgcagcggcg acgttaccgc ccttgccctg ctcgcgggcc
       61 gaggcttcgt tggtgctcgc cgcggccaac gtggtgccag cctcgtcgtc gatcacctgg
      121 agcacgatgt gcttgttgga acggaacacg gccaggcggg gacgctcggc ggtgccgtgg
      181 accttcttgc gcacccggtt gtggcgccgg atgcgtgcgt tgcggcgaag tttcgcgttg
      241 tcactcatgt cgtctctctg ccttacttct tgccggtctt gccggccttg cgcaggaccc
      301 gctcaccgag gtagcgcaca cccttgccct tgtagggctc gggcttgcgg acgctgcgga
      361 tgttggccgc gacctgaccg accacttctt tgtcgatgcc tcgcacgatg acccgagtgg
      421 gggtcggaac ctcgaaggtg atgccctccg gggcgtcgat cagcaccggg tggctgaagc
      481 cgagcgcgag gcgcagcttg gtggggctct gggcttcggc gcggtaaccg acgccgatga
      541 tctcgagctc cttggagaac ccgtcggtga cgccgacgac catgttgctg accagcgtgc
      601 gggtcaggcc atgcagcgaa cggttgtcgc gttcgtcgtt ggggcgctcg accagcaacg
      661 tgttggcgtc ggcgcggacg gtgatcgcac cgggcaccgc gaaggacagg ctgcccttgg
      721 gacccttgac ggacaccgtg cccgcgtcga tggcgacatc gacaccggac gggacggtga
      781 tgggggcttt gccgatacgg ctcatcagat acctcgaatc accacacgta gcacaggatc
      841 tcgccgccca cgttgcgctt gcgcgcctcg cggtcggaca gcaatccaga gctggtggac
      901 aggacggcca ccccgagacc accgagcacg cgaggcactt cggcagcctt gcggtacacc
      961 cgcagaccgg gggtggagac gcgcttgatg cccatgatgg tgcgggcgcg ctcgggcgtg
     1021 tacttcatct tcacggtcag gacctggccc ggacccttcg gcgccggagc gacggagtaa
     1081 ccatcgatga aaccctcctg ctggaggatc ttggcgaggg ccaccttctg cttcgacgac
     1141 ggcatcggga cctcgtcgtg catcgccgtg ttggcgttgc ggatgcgggt cagcatgtct
     1201 gcgatgggat cagtcaacat gggtactcac cagctcgcct tggtaaggcc gggcacttcg
     1261 ccggcgtggg ccatctcgcg caggcacacg cggcacaggc cgaacttgcg gtaaacggag
     1321 cgggcgcgcc cgcaacggcg gcaacgggtg tacccgcgca ccttgaactt cggcgtggca
     1381 gccgccttgt tgatcttcga cttcttggcc atggctgctc ctactacttc ttcttcttcg
     1441 ccttgggctg ggcagcgccc ttgccaccac ggaccgggcc acgacgcttc accttgcggg
     1501 gtgcggcggc tgcgtcggcg ccacgcttga acgggaaccc gaaggcatcg agcaacgccc
     1561 gtcccgcggc atcggtggtc gcggtggtga cgatggtgat gtccatgccg tggggggcat
     1621 cgatcttgtc gtagtcgatc tcggggaaca cgatctgttc cttgagaccc agggcgtann
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncaga
     1741 cggtcgagga actcgtacat gcggtcctgg cgcaaagtca ccttcgcacc gatcagctgg
     1801 ccttcacgca gcttgaacga cgcgatcgac ttcttcgact tggtgacgat cggcttctgg
     1861 ccggtgatct tctgcagatc gctgaccgcg ccctcgagca gcgatggctg ctgggtggcc
     1921 cgaccgacgc ccatgttgat gacgatcttc tcgaacttgg ggatctgcat cacgttggcg
     1981 atgccgagct gctctttgag agcagccttg acctcgtcct gatacttctg cttcaggcgg
     2041 ggaacggtgg cgatggctgt gtcgctcatc agatgacctc accggtgcgc ttggcgacgc
     2101 gcaccttggc gccgtcgtct tggatcttgt agccgacccg agtgggcttg cccttgtgca
     2161 ccagcatcac gttgctgaca tggatgggca tgtcgacctc gatgatgccg gcctgctggt
     2221 tctggctggt ggccttctgg tgcttggtgg cggtgttgac accgctgacc acgaccttgt
     2281 cgagcttggg gagcgccttg atgacttcgc cctcgcggcc cttgtccttg ccagagatga
     2341 ccacgacgtg gtcgcccttc ttgattctca tcacaacacc tccggggcga gcgagacgat
     2401 gcgcatgaac ttcttgtcgc gcagctcacg gccgacgggg ccgaagatgc gggtgccacg
     2461 cggggtgagg tcgtccttga tgatgacggc ggcgttctcg tcgaagcgga tgtagctgcc
     2521 gtcgggacgg cgccgctcct tcttggtgcg cacgacgacg cacttgacga cgtcgccctt
     2581 cttgacgccg gcgccgggga tggcgtcttt gacggtggcg acgaagatgt ctccgatccc
     2641 ggcataacgg cggcgcgtgc caccgagcac cttgatgcag agcacttcct tgccgnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnngctgg atcatgtcac ttcgcccttt ccacgatctc
     2761 gaacacgcgc cagcgcttca gcttgctgag cgggcgggtc tccatgacgc ggaccttgtc
     2821 gccgatgttc acgtcgttgg tctcgtcgtg ggcatacagc ttcttggtct gcatcatgaa
     2881 cttgccgtac ttggggtgac ggacgcgctc gaccacggcg atgaccgcgg tcttgtccat
     2941 cttgttggac acgaccacgc cctcgcggac cttgcgccgg ttgcgcacgg ccggcgcatc
     3001 agcggcggca gcgccagccg gggcagcctt ggcggcggcc ggtgctgcag tctcgggtgc
     3061 tgcagtttcg ggtgctgcgg tctcgggtgc tgcggtctcc ggtgctgggg tctccgcggt
     3121 ttcggatgtg tcgacggtgt tgtcttcgct catggcctgg ctcacttcgc cgactggacg
     3181 gcttcagcgg cagcgatttc cttctgccgg atgagggtgt tgatgcgggc gatctggcga
     3241 cgggtgttgc cgagggccgc agtgttctcg agctggcccg tcgcgtgacg gaagcgcagg
     3301 ttgagcgcct cctgcttggt ggcccgcagc tcgttgatga gaccggccag gtcgagctcg
     3361 gccagttcgg acttcttgga ccgcttggcc atgtcagatc acctcttcac gcgtgatgac
     3421 acgcgccttg atgggcagct tctgcacggc cttgttgagc gcggccttgg cctgctcctc
     3481 gttggggaag ctgagctcga acagcacccg gcccgggcgc acgacggcca cccagtattc
     3541 ggggttgccc ttgcccgaac ccatgcgggt ctcggccggc ttcttggtga ccggcttgtc
     3601 ggggaacacg ttgatccaga ccttgccgcc acgcttgatg gcacgggtca tggcgatacg
     3661 ggcagcttcg atctggcggg cggtgagcca acccggctcg agagcctgga tgccgtactc
     3721 gccctgggtc agcgtggcgc cgcccttggt gagaccggcg gtgcggccgc ggtgctgctt
     3781 gcggtgcttg accttacggg gcatcaacat gatggatcag tcctccccac ggaagtgcgg
     3841 tgcctcgtgg cccgcagcgg tacgacgagc gatctgctct tcttcgtcga gtaacttctc
     3901 gaactcggga tcggcttcct tcagcaacgg tgcggcctcg ggtgcatcgt cggcaccagg
     3961 gcggcgagcg ccgctggaca cgaccttgcg cggcgcaccg ctggtctcgc cgaccgccat
     4021 ggctgcctcg cgcaggatct tgtcgtcgtt ctggggcttg tagggcagga tgtcgccctt
     4081 gtagatccag accttgacgc ccacgcggcc gctggtggtc ttggcctcac ggaagccgta
     4141 gtcgatgtcg gcacgcagcg tgtgcagcgg cacgcggcct tcgcggtacc actcggtgcg
     4201 gctcatctcg gcgccaccga ggcggcccga acactggacg cggataccga gggcgccagc
     4261 cttctgggcg ttctgcacgg cccgcttcat ggcccggcgg aaggcgatgc ggttgatcag
     4321 ctggtcggcc acgccctgcg cgatcagcgc cgcgtcgagc tcggggctct tgatctcgac
     4381 gatgttgagc tgcaccttgg ggttgccggt gatcttggtg agcatggcgc gcaactcgtc
     4441 ggcctgggca ccacggcgac cgatcacgat gcccggacgg gcggtgtgga tgtcgacgcg
     4501 gagcttgtcg cgggtgcgtt cgatctcgat ccggctgatc gccgcggact cgagccgggt
     4561 catgatcgag cggcggatct tccagtcctc ggtgaggtat tccttgtagt cgcgctcgct
     4621 gaaccagcgg ctcttccagt cggtggtgat cccgaggcgg aagccgtagg ggttgatctt
     4681 ctgacccatg tcagttgcct tcctcagcgt cggcggcgct gtcgtcggat gcttcgg
//
LOCUS       DAORNF010000184         4707 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_13545_length_4707_cov_1.698194, whole genome shotgun sequence.
ACCESSION   DAORNF010000184 DAORNF010000000
VERSION     DAORNF010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4707
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13545_length_4707_cov_1.698194"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    248..409
                     /estimated_length=162
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <410..>623
                     /locus_tag="PLV68_08585"
     CDS             <410..>623
                     /locus_tag="PLV68_08585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRE01343.1"
                     /translation="DIVSRHGDLDVVILAFGVLGEQSEFDHDPVAAAEAVTANYTGSV
                     SLGLVVAEQLKRQGHGRLVVLSSVAGE"
     assembly_gap    624..735
                     /estimated_length=112
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <736..922
                     /locus_tag="PLV68_08590"
     CDS             <736..922
                     /locus_tag="PLV68_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01344.1"
                     /translation="HSAMTAGHKPAPFATTPAEVAKIAAAGIRRGRRVVWAPKVMRPM
                     FMVLRHLPGPVFRRLPM"
     assembly_gap    1128..1137
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1138..1585
                     /locus_tag="PLV68_08595"
     CDS             <1138..1585
                     /locus_tag="PLV68_08595"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRE01345.1"
                     /translation="PRNGRPIMVERGRIPVDAASIVVGLDHSQGAQRALQFACAEAAD
                     KLLLVHVVIPGTARADRRAAAAWLDDIAQRCADAHDRDARTVEARLIDGWPGPELAAV
                     SEDADVITVGSRGITGFRARHLGSVATYLATHAACPVVIHHPPRHD"
     gene            complement(1626..2747)
                     /locus_tag="PLV68_08600"
     CDS             complement(1626..2747)
                     /locus_tag="PLV68_08600"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRE01346.1"
                     /translation="MTPRSLRAALGHRCLALAAVAALTALAACSSDTEPADSTPQSSL
                     IPAERCAANKAVGTITYLSGFDFAATPGIVDVVMAHSRGYYADLCLDVELRSSFSTAN
                     YPLVASNTAQFASAGNYTEIINQSGNGAEFVAVINDGKAPIEALITPAGGATTLEALR
                     GKKIGFKGDIPPSIVAMLAKAGLQRGTDYTEISLDGFDPVAHIRSGVDAFPVYKSNEP
                     WQLQSAGIEFNLFDPATDGIPGSFGIVYTSKGFVTAHPQATADFVRATLRGMADTIVD
                     PAAAVAASVALINAGGNTNFLTEAGETFRLSEDLRLVAASTPAGQPVGVIDPALFDAE
                     YAAYVAAGVWPDGAPADGHYYDADLAASLYDANSTVIWP"
     gene            complement(2771..3538)
                     /locus_tag="PLV68_08605"
     CDS             complement(2771..3538)
                     /locus_tag="PLV68_08605"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRE01347.1"
                     /translation="MKTLGARLLAPVLGVIAFFAAWQAAIEVWNIPKYALSSPLDILR
                     SLRTSPRFYFDQARPTLWAALLAFVLSLLIGTVAASIMASSRFVERAASPLIVLVQVT
                     PILAYAPAVVIWLGYDLAPILFLTVLISTVPFIANGVTGLRSVAPLLLELAHSVDARR
                     WEVFVRLRLMSALPSLFSAARIAVGTVLIGVVIGEWFAGVSSGLGYTIKVASNRNLPL
                     VMWASVFVLGTLGALTIVLIGLLERIVLHWHASQRRA"
     gene            complement(3535..4389)
                     /locus_tag="PLV68_08610"
     CDS             complement(3535..4389)
                     /locus_tag="PLV68_08610"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRE01348.1"
                     /translation="MRADESMTTTTAAIEVRGLGKVFRGRVRAVSALDGVELRVGHGE
                     FVTLLGPSGCGKSTLLRAIGGLLRPDSGSVTIAGVSADQARAAKQFALVPQAPALVPW
                     KTLAQNVRFLATLHRGAHAHPVLADAAIDALLSRVGLAGFEQAYPHELSGGMQQRASL
                     VRAFALGAPVLLMDEPFAALDEITRSEMRYLLADLRADPETAGPETASATATTPATTV
                     VFVTHSLAEAVVLSDRVAVMASRPGRIVAVHDIPLARPRTPDLEDSPQFHALVKALRA
                     DLHEHRER"
     gene            4484..>4707
                     /locus_tag="PLV68_08615"
     CDS             4484..>4707
                     /locus_tag="PLV68_08615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01349.1"
                     /translation="MQTQPRPAYVGQSYTGRGRWRGIESFIVLVAAMVGVQSVTHAAL
                     ISLAPGYSSAVSALIETAAVTSVLAIGAGQ"
BASE COUNT          696 a         1452 c         1584 g          691 t
ORIGIN      
        1 tcacacccca cggccgaggc gacccgccgt ggctacccgc gcctggccga gtggcaggcc
       61 gtgcgcgacc gcatcgaccc cgatcgggtg tggcagagcg acctcggtcg ccgtctcggt
      121 ctgatgtgat ccgctcgccg tcacactgtt catgtcatca cgtcaatcga actcggagaa
      181 gatcgtgcag aactcgttcg gagaacctca aaccgtcgtg ctcttcggcg gcaccagcga
      241 catcggcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc gacatcgtgt
      421 ctcgccacgg cgatctcgac gtcgtcatcc tcgccttcgg tgtgctcggc gagcagagcg
      481 agttcgatca cgacccggtc gcggccgccg aggcggtgac ggccaactac accggttcgg
      541 tcagcctcgg attggtcgtc gccgaacagc tcaagcgcca gggtcacggg cggctggtcg
      601 tgctgtcgag cgtggcgggc gagnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnnccact cggcgatgac ggccgggcac aagccggcgc cgttcgcgac
      781 cacacctgcc gaggtggcca agatcgcggc ggcaggcatc cgccggggcc gccgcgtcgt
      841 ctgggcgccg aaggtgatgc gaccgatgtt catggtcctg cgccatctgc ccggaccggt
      901 cttccgtcgc ctcccgatgt agtcgacacg ccatccgggc agtttcaggt cggaagtccc
      961 tggtggctgg cctgttctgg cgccagcctg ggacctgtgc actggaactc aatcgaccca
     1021 ctggtggtcg acgctgctgc gtggggtggc agcgtcgcgc cagtgggctc gatcggcatc
     1081 agccgatcgg agcgttgcgt ggcaggttca ggcgcgtggg gtgtcgannn nnnnnnngcc
     1141 acgcaacgga cgtccgatca tggtcgagcg gggacggatt cccgtcgatg ccgcctcgat
     1201 cgtggtcggg ctcgatcact cacagggagc acaacgggcg ctgcagttcg cctgtgcgga
     1261 ggcggcggac aagctgctgc tcgtccacgt cgtgatccct ggcacggcgc gcgccgatcg
     1321 ccgcgccgcg gccgcctggc ttgacgacat cgcgcagcgt tgcgccgatg cacatgaccg
     1381 cgatgctcgc acggtcgagg cccggctcat cgacggctgg ccgggtcctg aacttgccgc
     1441 ggtcagcgag gacgccgacg tgatcacggt cggttcgcgg ggcatcaccg ggttccgcgc
     1501 ccgccacctc ggatcggttg cgacgtatct ggccacgcat gccgcgtgtc cggtggtgat
     1561 ccaccacccg ccccgccacg actaggccgc gctatccctg acggtggcgt tccctgacgc
     1621 tggcgttacg gccagatcac cgtgctgttg gcgtcgtaga gcgacgcggc gaggtcggcg
     1681 tcgtagtagt ggccgtcggc gggggctccg tcgggccaca ccccggctgc gacgtaggcc
     1741 gcgtactcgg cgtcgaacag cgccgggtcg atcaccccga ccggctgacc tgccggtgtc
     1801 gaagcggcga cgaggcgcag gtcctcgctc aggcggaagg tctcacccgc ttcggtcagg
     1861 aagttggtgt tgccgcctgc attgatcagc gccacgctgg ccgcgaccgc cgcggcgggg
     1921 tcgacgatgg tgtcggccat gccgcgcaac gtggcacgca cgaagtcggc ggttgcctgc
     1981 gggtgcgcgg tcacaaaacc cttcgacgtg tagacgatgc cgaagctgcc ggggatgccg
     2041 tcggtggctg ggtcgaacag gttgaactcg atgcctgccg actgcaactg ccacggctcg
     2101 ttcgacttgt acacggggaa ggcgtcgacg ccggacctga tgtgggcaac cgggtcgaag
     2161 ccgtcgaggc tgatctcggt gtagtccgta ccgcgttgca gacccgcctt ggcgagcatc
     2221 gccacgatcg agggcgggat gtcgcccttg aagccgatct tcttgccgcg caacgcttcg
     2281 agtgtcgtcg cgccaccggc cggggtgatc agcgcctcga tcggagcctt gccgtcgttg
     2341 atcaccgcga cgaactcggc gccgttgccg ctctggttga tgatctcggt gtagttgccg
     2401 gccgaggcga actgcgcggt gttcgacgcg acgaggggat agttggcggt ggagaagctg
     2461 gaccgcagtt cgacatcgag gcacaggtcg gcgtaataac cgcgcgaatg ggccatcacc
     2521 acgtcgacga tcccgggcgt ggcggcaaag tcgaacccgc tgagataggt gatcgtgccg
     2581 actgccttgt tggccgcgca gcgttcggcc ggaatcagcg aactctgcgg agtgctatcc
     2641 gctggctcgg tgtcgctgct gcacgccgcg agggcggtga gcgcggcgac ggcggcgaga
     2701 gccagacagc ggtggccgag tgcggcccga agcgatcgtg gggtcatgag ggcagggtaa
     2761 cgaattgccg tcacgcgcgg cgctggctgg cgtgccagtg cagcacgatc cgttcgagca
     2821 ggccgatcag cacgatcgtc agcgctccca gcgtccccaa cacgaacacc gacgcccaca
     2881 tcaccagtgg caggttgcgg ttcgacgcga ccttgatcgt gtagccgagc ccggagctga
     2941 ccccggcgaa ccactcgccg atcaccacac cgatcaacac ggtgccgacc gcgatcctgg
     3001 ccgcggagaa caggctcggc agggccgaca tcaggcgcag gcgcacgaac acctcccacc
     3061 ggcgggcatc gaccgagtgg gccagttcga gcagcagcgg agcgaccgac cgcagcccgg
     3121 tgacgccgtt ggcgatgaac ggcacggtgc tgatcaacac ggtcaggaac aggatcgggg
     3181 ccaagtcgta ccccagccag atcaccacgg cgggagcgta ggccaggatc ggggtgacct
     3241 ggaccagcac gatcagcggt gacgcggctc gctcgacgaa gcgcgaactg gccatgatcg
     3301 atgcggccac cgtgccgatc aacagtgaca gcacgaacgc cagcagcgcc gcccacagcg
     3361 tgggccgggc ctggtcgaag tagaagcgag ggctggtcct cagggaacgg aggatgtcga
     3421 gcggcgacga caaggcgtac ttggggatgt tccacacctc gatcgccgcc tgccatgcgg
     3481 cgaagaaggc gatcaccccg aggacgggcg cgaggagacg ggcaccgagg gtcttcatcg
     3541 ctcgcggtgt tcgtgcaggt cggcccgcag cgccttgacc agcgcgtgga actgcggact
     3601 gtcttcgagg tcgggcgtgc gcgggcgggc gagggggatg tcgtggaccg cgacgatgcg
     3661 tcctggtcgg gaggccatga cggcgacccg atcggagagc acgaccgctt cggccaggct
     3721 gtgggtgacg aacacgaccg tggtcgcggg cgtcgtcgcc gtggcgctgg ctgtctcggg
     3781 gccggctgtc tcggggtcgg cgcgcaggtc ggccagcagg tagcgcatct cggagcgggt
     3841 gatctcgtcg agtgcggcga agggctcgtc catcagcagc actggcgcac cgagcgcgaa
     3901 ggcccgtacc aacgaagccc gttgctgcat gccgccggac agttcgtgcg gataggcctg
     3961 ctcgaaaccc gccagcccga cgcggctcag cagcgcgtcg atggccgcgt cggcgagtac
     4021 gggatgggcg tgtgcgccac ggtgcagcgt ggccagaaag cgcacgttct gagccagcgt
     4081 cttccacggc accagtgccg gtgcttgcgg gacgagcgcg aactgcttgg ccgcccgtgc
     4141 ctgatcggcg ctgacgcccg cgatcgtgac cgaccccgag tcgggccgca gcagaccgcc
     4201 gatggcccgt agcagcgtgc tcttgccgca gccgctcggg ccgagcaagg tgacgaactc
     4261 gccgtgaccg acgcgcagct cgacgccgtc gagcgcggac acggcacgga cgcgcccgcg
     4321 gaacaccttg cccagtcctc ggacctcgat cgccgccgtg gtcgtcgtca tcgactcgtc
     4381 ggctcgcacc cgagcgagtc tacgtgtcca ggcgtcggca aggttgacaa cattttcgat
     4441 tgtggtggcg ggtcgcggtg ccgatgaaac cgccgtggac agcgtgcaga cccaaccgcg
     4501 tcccgcgtac gtggggcagt cgtacacggg acggggccgt tggcggggga tcgagtcgtt
     4561 catcgtgctc gtcgccgcga tggtcggggt gcagtcggtc acccacgccg ccctgatctc
     4621 gctcgcgccc gggtactcct cggcggtgtc ggcgttgatc gagaccgccg ccgtgaccag
     4681 tgtgctcgca attggcgcgg gacagtg
//
LOCUS       DAORNF010000185         4706 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_13555_length_4706_cov_1.603956, whole genome shotgun sequence.
ACCESSION   DAORNF010000185 DAORNF010000000
VERSION     DAORNF010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4706
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13555_length_4706_cov_1.603956"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>351)
                     /locus_tag="PLV68_08620"
     CDS             complement(<1..>351)
                     /locus_tag="PLV68_08620"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRE01350.1"
                     /translation="NPKITDKLTKELVLKFTAADLPEGNRGWYYDDKGDQYYPFPAPA
                     GSMVVQNYQTGQIVAMASYPNFDNRWFTVGLTSKKFEVLFPATKDPDKSTLVNRAIQG
                     QYNMGSTFKPVVALA"
     assembly_gap    352..471
                     /estimated_length=120
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<472..1159)
                     /locus_tag="PLV68_08625"
     CDS             complement(<472..1159)
                     /locus_tag="PLV68_08625"
                     /inference="COORDINATES: protein motif:HMM:NF015662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01351.1"
                     /translation="MAVDKRAARLGALALVSVILFGVVGTRLWFLQTVQRKDVLERVE
                     RSRTRTQVLVPERGRIFDAWGRILADNRRVLTVTVDRAVIRKKSQRLELFTRLSGIVN
                     VPVDVMEDRYNSGRYSQYLPLPIAEDVDEDVVLRLESRSEDYPGVTGEEDWERVYPYA
                     PLASHVVGYMGLILKNQATEYLAKGYLNNEVIGQFGIEKSMEDVLHGEWGLVVYEVDS
                     SNRVVREISRR"
     assembly_gap    1226..1305
                     /estimated_length=80
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1306..1737)
                     /gene="mreD"
                     /locus_tag="PLV68_08630"
     CDS             complement(<1306..1737)
                     /gene="mreD"
                     /locus_tag="PLV68_08630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HRE01352.1"
                     /translation="MLRFLRRPTLRIVPVGLIVMGVQLSWFRTVQPFDTVIQIVLALA
                     AASGIRGGAEKGAWMGFVLGAMFDLGSGATLGQHALAYGLAGFVAGLVSMVAVDPHWW
                     LSMLFVAAGATVGELMVPVINTFLSGSGGWQGGRLATILPVT"
     gene            complement(1741..2958)
                     /gene="mreC"
                     /locus_tag="PLV68_08635"
     CDS             complement(1741..2958)
                     /gene="mreC"
                     /locus_tag="PLV68_08635"
                     /inference="COORDINATES: protein motif:HMM:NF016011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HRE01353.1"
                     /translation="MPVFSPSRRRAIVMLLLTSILLLTLDLRGNALVSRARDGFSVVL
                     QPFVTAGDVIATPVKNAWHGATDYQELQRENERLREQIAQQRGDDIAARAILAAHQEI
                     IALQVLAANYPRVTARVVGDRTSNFSETIEIDRGSADAIKEGMAVISRAGLVGKITKV
                     YPNRSIVRLITDAEYAIECKVTGVPDIDPFTGQPIEDDDGTTEATVASGMTESDLTST
                     TAAAPPVDPEAGGTGDGTTTGDGTGNGTGAGTGTGTGSDGTGTNAGGTPGTGTSGDGT
                     SGDSTSGDGTDGGETGDGTDPGTTTTTLAPELVLRETGGCQGRGRDRLPVMRYVTENP
                     AFSGVQNGDVITTAGGSASLVPPDIAIGVVINRIERTHSAGPLLEIELAADLDHLNFV
                     QVVLYQPPLEASG"
     gene            complement(2977..4000)
                     /locus_tag="PLV68_08640"
                     /pseudo
     CDS             complement(2977..4000)
                     /locus_tag="PLV68_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441397.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
     gene            complement(4058..>4387)
                     /locus_tag="PLV68_08645"
     CDS             complement(4058..>4387)
                     /locus_tag="PLV68_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002515614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HRE01354.1"
                     /translation="AKNLTVVALDLRQLDADTLARHYEEHVGKGFYGELVEFMSRGPV
                     VAMVVEGPEDTWEVVRTMMGATNPRKAAPGTIRGDLGILFTENLVHGSDSLESAQREI
                     AIFFPNL"
     assembly_gap    4388..4425
                     /estimated_length=38
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4426..4480)
                     /locus_tag="PLV68_08650"
     CDS             complement(<4426..4480)
                     /locus_tag="PLV68_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002515614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HRE01355.1"
                     /translation="MSDRTLVLLKPDAVERKL"
BASE COUNT          694 a         1497 c         1493 g          784 t
ORIGIN      
        1 gccagtgcga ccaccggctt gaacgtcgag cccatgttgt actggccttg gatcgcacgg
       61 ttgaccaagg tcgacttgtc ggggtctttg gtcgcgggaa acagcacctc gaacttcttg
      121 ctggtcaacc ccacggtgaa ccaccggttg tcgaagttgg gatagctggc catcgccacg
      181 atctgaccgg tctgatagtt ctggaccacc atcgaaccag cgggtgcagg gaacgggtag
      241 tactgatcac ccttgtcgtc gtagtaccag ccgcggttgc cctcgggcag gtccgcggcc
      301 gtgaacttga ggacgagctc cttggtgagc ttgtcggtga tcttcgggtt cnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncgcgccgcg
      481 agatctcgcg caccacgcgg ttggagctgt cgacctcgta gacgaccagg ccccattcgc
      541 cgtggagcac gtcctccatg ctcttttcga tgccgaactg gccgatcacc tcgttgttga
      601 gatagccctt ggccaggtac tcggttgcct ggttcttgag gatcaggccc atgtagccga
      661 cgacgtggct ggccagcggg gcgtaggggt agacccgctc ccaatcctct tcacccgtga
      721 cgcccgggta gtcctcgctg cggctctcca ggcgtaacac gacgtcttcg tcgacgtcct
      781 cggcgatcgg cagcggcagg tactggctgt agcggccgct gttgtagcgg tcctccatca
      841 cgtcgaccgg cacgttgacg atgcccgaca ggcgggtgaa cagctccaac cgctggctct
      901 tcttgcggat caccgcccga tcgacggtca cggtgagcac gcggcggttg tcggccagaa
      961 tgcggcccca ggcgtcgaag atgcggcctc gttcgggtac cagcacctgg gtccgggtgc
     1021 gcgaccgctc gacgcgttcg agcacgtcct tgcgctgcac ggtctgcaag aaccacaagc
     1081 gggtccccac gaccccgaac aggatcacgg acacgagggc gagcgcgccc agacgagcgg
     1141 cgcgtttgtc gacggccatg tgcttattgt gctcgctcgg gcgacagcga tgggcgcggg
     1201 ctgttcacgg gttgatcgcc ttccannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnggtga ccggcaggat
     1321 cgtggccagc cggccgccct gccaaccacc ggagccgctg aggaacgtgt tgatcacggg
     1381 gaccatcaac tcgcccaccg tggccccggc ggccacgaac agcatcgaca accaccagtg
     1441 cgggtcgacc gcgaccatgc tgaccaaccc ggcgacgaac ccggccagcc cgtaggccag
     1501 cgcatgctga ccgagtgtgg cgccgctgcc gagatcgaac atcgcgccga gcacgaaccc
     1561 catccaggca cctttctcgg ctccaccgcg gatacccgac gcggccgcca gggcgagcac
     1621 gatctggatc acggtgtcga acggctgcac cgtacggaac cacgacagtt gcacgcccat
     1681 cacgatcaag ccgaccggga cgatgcgcag ggtcggtcgg cgcaggaacc gcagcacggt
     1741 tcagcccgac gcttccagcg ggggctggta caggaccacc tggacgaagt tgagatgatc
     1801 gagatcggcc gccaactcga tctcgagcaa cggcccggca gagtgggtgc gctcgatgcg
     1861 gttgatcacc acgccgatgg cgatgtccgg aggcaccagg ctggccgacc caccagcggt
     1921 ggtgatgacg tcaccgttct gcaccccgct gaaagccggg ttctcggtga cgtaacgcat
     1981 caccggcagg cgatcacggc cgcggccctg gcagccgccg gtctcgcgca gcaccagttc
     2041 tggagccagg gtcgtcgtgg tggtgccggg gtccgtgccg tctccggtct cgccgccgtc
     2101 cgtgccgtca ccgctcgtgc tgtcaccgct ggtgccgtca ccgctcgtac cggtaccagg
     2161 cgtaccgcca gcgttcgtgc cggtgccgtc gctgccagtg ccggtgccgg tgccggcgcc
     2221 agtgccattg ccagtgccgt cgccggtcgt ggtgccgtcc ccggtgccac cagcctctgg
     2281 atcgacgggc ggggcggccg cggtggtcga ggtcagatcg gactcggtca tgccgctggc
     2341 gacggtcgct tcggtggtgc cgtcgtcgtc ctcgatgggc tggccggtga acgggtcgat
     2401 gtcgggcacg ccggtgacct tgcactcgat ggcgtactcg gcgtcggtga tcaggcgcac
     2461 gatgctgcgg ttggggtaga ccttggtgat cttgccgacc agaccggccc ggctgatcac
     2521 cgccatcccc tctttgatcg cgtccgccga tcccctgtcg atctcgatcg tctcgctgaa
     2581 gttgctggtc cggtcgccga ccacgcgagc ggtcacccgt gggtagttgg cggccaggac
     2641 ctgcagggcg atgatctcct ggtgggccgc gaggatcgcc cgggccgcga tgtcgtcgcc
     2701 gcgctgctgg gcgatctgct cgcgcaggcg ctcgttctct cgctgcagtt cctggtagtc
     2761 ggtcgccccg tgccatgcgt tcttgaccgg cgtggcgatg acgtcgccgg ctgtgacgaa
     2821 cggctgcagc acaacgctga acccgtctcg ggcccggctg accaacgcgt tgccgcgcag
     2881 gtcgagggtc aacagcagga tcgacgtcaa caacagcatc acgatggcgc ggcggcgact
     2941 gggcgaaaat accggcatgg cgtcagccag tctggactat tcgtcggcgc cttgggacag
     3001 cagccctcgc atcgcgtcga tgttctccaa ggcccggccc gacccgatga ccacgctgta
     3061 caggggttct gcagcgatca ggatgggcat cccggtctcg tgtgacaagc gctggtccag
     3121 cccggccagc agcgcgccac cgccggtgat catgatgccc tggtgcatga tgtcggcggc
     3181 cagttccggc ggcgtcttgt cgagggtggt cttgaccgcg tcgacgatgg ccgcgaccgg
     3241 ctcggcgatg gcttcccgca cctgttcggt ggtgacctcg agggtacgcg gcaggccgct
     3301 gatgaggtcg cggccgcgga tgtcggcggt gagctcttcg tcgagcggcc aggccgaacc
     3361 catgtgcagc ttgatctcct cggcggtgcg ctcccccacg gcgaggctga actccttctt
     3421 gaggaactgc gagatcgcgt cgtcgagctc gtcacccgcg acgcgcaccg actgggccgt
     3481 gacgatgcca ccgagggaga tgacggcgac ttcggtggta ccaccaccga tgtcgacgat
     3541 catgttgccg ctgggctcgt gcaccggcag gtcggcgccg atcgcggcct tcgatgatgt
     3601 gcaccggctt gcgcgccccg gcgaactcgg ccgcatcctg caacgcacgc tgttcgaccc
     3661 cggtgatgcc cgacgggacg cagatcacca tccgcggctt gctccacttg ctggcgtgca
     3721 ccttctggat gaagtagcgg agcatcttct cgcacacgtc gaaatcggcg atcacaccgt
     3781 ccttcagcgg gcggaccacc ttgatgtgac ccggcgtgcg ccccatcatg cgcttggctt
     3841 cgagaccgac cgcgacgggg cggccgtcgt tgacgttgag cgcgacaaca gacggctcgt
     3901 tgagcacgac gccctggcca cgggcataga tcaaagtgtt ggccgtgccc aggtcgatcg
     3961 cgagatcacg cccgagggca agcggattgt tgcgtgccat ggtgggccct tcagacggca
     4021 acccgggatg cgctcactgt agcgtgcccc tggtttgtca caggttcggg aagaagatcg
     4081 cgatctcccg ctgggccgac tcgagcgagt cgctgccgtg gaccaagttc tcggtgaaga
     4141 ggatgccgag atcaccgcga atggtgccgg gcgcggcctt gcgcgggttg gtggcaccca
     4201 tcatggtgcg cacgacctcc cacgtgtctt ccggaccttc gaccaccatc gccaccacgg
     4261 gaccacggga catgaactcg accagttcgc cgtagaagcc cttgccgaca tgctcttcgt
     4321 agtgacgagc cagggtgtcg gcgtcgagtt ggcgcagatc cagggcgacg acggtcaggt
     4381 tcttggcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncgagc ttgcgctcga
     4441 cggcatcggg cttgagcagg accagtgtgc gatcagacat ggcgcacaac gctacctgcc
     4501 gaccgccttc aagccatcac cttcgggggc tgcgcccccg cttcgcgccc catcaccgcc
     4561 cggctcgctg gcgctcgcac ggtcggggcg ctcgctacgc tcacgccatc cgttgcgcaa
     4621 tgaggaaggg gcgggcggcg ccgacgacgt acaggctgcc cgcgacgagg atcgcgtcgt
     4681 cgaccccggc gttgtccttg gcccgc
//
LOCUS       DAORNF010000186         4702 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_13574_length_4702_cov_1.875619, whole genome shotgun sequence.
ACCESSION   DAORNF010000186 DAORNF010000000
VERSION     DAORNF010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4702
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13574_length_4702_cov_1.875619"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1297)
                     /locus_tag="PLV68_08655"
     CDS             complement(<1..1297)
                     /locus_tag="PLV68_08655"
                     /inference="COORDINATES: protein motif:HMM:NF023702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal domain-containing
                     protein"
                     /protein_id="HRE01356.1"
                     /translation="MSEWVMLADLCFADLLLYVPAGAGRWIVAAHVRPATGQTIYHSD
                     WIGAWASDSEMPLLAAVVEDGQVHEGEITVASLPAPAQMLAIPVRFRGEIIAVMTRER
                     TGISSRQPGELERTYLSLFDRFATMITQGRFPFPGRIADSGAAPRVGDGAMVLDAEAR
                     VRFASPNAVSALHSVGISANAVGMRLAELGFNDSSVRQAYERVEPVIQEFDQNPDVTL
                     LSRCIPIIDGGEVTGGILLVRDVTELRKRDRMLLSKDATIREIHHRVKNNLQTISSLL
                     RLQARRLSSDEAKAAVAESVRRIRTIALVHETLSREPGDDVAFIEIVRPLLRLAEEGL
                     QSVDRPVHFTVQGDGGRLPSTVATPLSVVLTELLQNAVDHGFPEDSPGGRVVVQLAND
                     GTDLRIRVLNDGADLDPGFDLTKATGLGLSIVRTLVTTEL"
     gene            complement(1428..1646)
                     /locus_tag="PLV68_08660"
     CDS             complement(1428..1646)
                     /locus_tag="PLV68_08660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01357.1"
                     /translation="MAKAVGAEPDTVASLLGEIAAAFADTAVVATREAERRSELERLV
                     PFVAVAPALDQDIHNLASLLELGDHLWG"
     gene            complement(1652..2662)
                     /locus_tag="PLV68_08665"
     CDS             complement(1652..2662)
                     /locus_tag="PLV68_08665"
                     /inference="COORDINATES: protein motif:HMM:NF014433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HRE01358.1"
                     /translation="MTMPTAPRPTRPATPTGQPIRRLPRSVAATDVGVANLLAAKEMV
                     IVCGSGGVGKTTVAAALAAEAAIHIGGRVLVLTIDPAKRLANALGLHEFGNTEIRIAP
                     EQFAAAGVKPRGELWAAMLDTKAGWDELIRRHAPDATLRDTVLANPLYQNITGRFVQS
                     HDYIAMERLHDLHASGEFDLVVIDTPPSRNALDILDAPGRMMEFFGSRLLRWLTVPYR
                     SRLFTAASKPFYQVADRVLGSRFLQDIAEFFVLFQTMEKGFVARARDVERLLSDPCTS
                     FLVVSTLEAAPAHEAAYLAAALAERDLPLGGMILNRAMPAIDSPRPRPMRSPPWPSRP
                     VR"
     gene            complement(2669..3610)
                     /locus_tag="PLV68_08670"
     CDS             complement(2669..3610)
                     /locus_tag="PLV68_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HRE01359.1"
                     /translation="MNLLDRQLLFVTGKGGVGKTTVAAAIAQLAAQHGRSTLVCEMDA
                     KGSLATAFDCSPFTFQPRRIDTGLFGMAMNTEDSLREYLRLFVRIPFVGRIGPLARTF
                     DFVADAAPGVKEILAVGKLCYEVRERHYDLVVVDAEASGHIVAQIGAPRVIGELVQVG
                     LVKDQTAWMLDILEDPQRTGLITVTTPEEMPVNETLDLLASVRERTNVDPAAVVVNRV
                     LPALFGAREAPVFERLFEPEPTELLVDAIGPAVVDVLAAARTIESRRHVGDAHLDRLR
                     TELGPDLLAVYIPELFTRATGRRVVSLVTEALADELL"
     gene            complement(3646..3753)
                     /locus_tag="PLV68_08675"
     CDS             complement(3646..3753)
                     /locus_tag="PLV68_08675"
                     /inference="COORDINATES: protein motif:HMM:NF022749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3107 family protein"
                     /protein_id="HRE01360.1"
                     /translation="MWVTDKKGREIGVSAAKIAYVDIGSADHVRRVGFG"
     gene            complement(3771..3920)
                     /locus_tag="PLV68_08680"
     CDS             complement(3771..3920)
                     /locus_tag="PLV68_08680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01361.1"
                     /translation="MLRPLGSRQERTAVDLRIGVTDIARELTVELADENSGIESRIDV
                     VEPTR"
     gene            3959..>4353
                     /locus_tag="PLV68_08685"
     CDS             3959..>4353
                     /locus_tag="PLV68_08685"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRE01362.1"
                     /translation="MTAAATPPAAATPATATSANATPRRPRLRAADRRTLILEAARRA
                     FSASGDVRGTTVKQIAEEAGISEGILYRHFDSKDELFVQAAVEPLTAAMQTIIDSVSG
                     LDLTKFGPELHDSSVQYWQETIDAVTELVP"
     assembly_gap    4354..4386
                     /estimated_length=33
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4387..>4702
                     /locus_tag="PLV68_08690"
     CDS             <4387..>4702
                     /locus_tag="PLV68_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01363.1"
                     /translation="YAVPFYRDVLAPALKQMRDSWDAIYIREFGEPFPDRENATSHIG
                     TIVMLALERRLAATPRTSYDMALQLVATEEFHLRSAIAAVSRRRTPGTKAPSTKTTGT
                     KPT"
BASE COUNT          769 a         1592 c         1605 g          703 t
ORIGIN      
        1 tcagctcggt ggtcaccagc gtgcgcacga tcgacagccc caggccggtg gccttggtga
       61 gatcgaagcc gggatcgagg tcggcgccat cgttcaacac gcggatgcgc aggtcggtgc
      121 cgtcgttggc cagctgcacc accacccgcc cgccggggct gtcctcgggg aagccgtgat
      181 cgacggcgtt ctggagcaac tcggtcaaca ccacgctgag cggggtggcc accgtggacg
      241 gcagccggcc accgtcaccc tgcacggtga agtgcaccgg gcggtcgacc gactgcaggc
      301 cctcttcggc caggcgcagc agcggacgga cgatctcgat gaaggcgaca tcgtcgcccg
      361 gttcgcgcga caacgtctcg tgcaccagcg cgatcgtgcg aatgcgccgc accgactcgg
      421 ccaccgcggc cttggcctcg tcggacgaca gccgccgcgc ctgcagccgc aacagcgacg
      481 agatcgtctg caggttgttc ttgacgcggt ggtggatctc gcggatcgtc gcgtccttcg
      541 acagcagcat gcggtcacgc ttgcgcagct cggtgacgtc gcgcaccagc aggatgcctc
      601 cggtcacctc gccgccgtcg atgatgggga tgcaccggct gagcagggtg acatcggggt
      661 tctggtcgaa ctcttggatc accggctcga cccgctcgta ggcctggcgc accgagctgt
      721 cgttgaagcc caactcggcc aggcgcatgc cgaccgcgtt ggcgctgatg ccgacgctgt
      781 gcagggccga cacggcattg ggtgacgcga agcggacccg cgcttcggcg tcgagcacca
      841 tcgcgccgtc gcccacccgg ggagcagcac cggagtcggc gatgcggcct ggaaagggaa
      901 atctgccctg ggtgatcatg gtggcgaagc ggtcgaacag cgagaggtag gtccgctcga
      961 gctcgcccgg ctggcgactg ctgatgccgg tgcgctcgcg ggtcatcacc gcgatgatct
     1021 cgccccggaa gcgcacgggg atcgccaaca tctgcgcagg cgccggcagg ctggccacgg
     1081 tgatctcacc ttcgtgcacc tgaccgtctt cgaccacggc ggccagcagc ggcatctcgc
     1141 tgtcgctcgc ccacgccccg atccagtcgc tgtgatagat ggtctggccc gtggccggcc
     1201 ggacgtgggc ggcaacgatc cagcgtcccg cgccggccgg cacgtagagc agcaggtcgg
     1261 cgaagcacag gtcggcgagc attacccatt cggacaacca ggtggtgcag atgcgcggtc
     1321 tgttcgcgcg acaaggtggt gtgctgtctc gtcagttcgg cgagggtggc catgactgct
     1381 cccggcgtgg gtctgggtgg ttcggtggtt gagtttggcg catctcgtca gccccagaga
     1441 tgatctccga gctcgagcaa gctggcgagg ttgtggatgt cctggtcgag tgccggtgcc
     1501 accgcgacga acggcaccag ccgctccagc tcactgcgcc gttcggcctc gcgggtggcc
     1561 accaccgcgg tgtcggcgaa tgccgcggcg atctcgccga gcaggctcgc caccgtgtcg
     1621 ggttcggcgc cgaccgcctt ggccacagcg gtcaacgaac ggggcgactc ggccagggcg
     1681 gcgagcgcat cggccgcggc cgcggtgaat cgatcgccgg catcgcccgg ttgaggatca
     1741 tgccgcccag ggggagatcg cgttcggcca gcgccgcagc cagataggcg gcctcgtggg
     1801 ccggtgcagc ctccagcgtg gacaccacca gaaagctggt gcacggatcg gacagcagcc
     1861 gctcgacgtc gcgcgcccgc gcgacgaagc ccttctccat cgtctggaac aacacgaaga
     1921 actcggcgat gtcctgcaag aaccgcgagc ccaacacccg gtcggccacc tgatagaagg
     1981 gcttcgacgc ggcggtgaac agacgcgagc ggtacggcac ggtgagccag cgcaagaggc
     2041 ggctaccgaa gaactccatc atccgaccgg gcgcgtcgag gatgtcgagc gcgttgcgcg
     2101 acggcggcgt gtcgatcacc accaggtcga actcaccgct ggcgtgcagg tcgtgcaggc
     2161 gctccatggc gatgtagtcg tggctctgca cgaaccgccc ggtgatgttc tggtacaacg
     2221 ggttggccaa cacggtgtcg cgcagcgtgg cgtcgggtgc gtggcggcgg atcaactcgt
     2281 cccagcccgc cttggtgtcg agcatcgcag cccacagctc gccacgcggt ttgacgccag
     2341 cggcggcgaa ctgctcgggg gcgatgcgga tctcggtgtt gccgaactcg tgcaggccga
     2401 gcgcattggc caggcgcttg gcgggatcga tggtcagcac cagcacccgg ccgccgatgt
     2461 gaatcgccgc ttcggctgcc aacgcggccg ccacggtggt cttgccgact cccccgctgc
     2521 cgcagacgat caccatctcc ttggccgcca acaggttggc gacgccgaca tcggttgccg
     2581 cgacagaccg cggcaggcgg cgaatcggct ggccggtcgg cgtggcgggg cgggtcgggc
     2641 gcggcgcggt cggcatcgtc atcggctctc agagcagctc gtcggcgagg gcttcggtca
     2701 ccagcgacac gacccgtcgg ccggtggcgc gcgtgaacag ctcggggatg tagacggcca
     2761 gcaggtctgg ccccaactcg gtgcgcagcc ggtcgaggtg ggcatcgccg acatgacggc
     2821 gcgactcgat ggtacgggcc gcggccaaca cgtcgaccac ggccgggccg atcgcgtcga
     2881 ccagcagctc ggtcggctct ggttcgaaca gccgctcgaa aaccggcgcc tctcgagcgc
     2941 cgaacaaggc gggcaacacg cggttgacca ccaccgcggc cgggtcgacg ttggtgcgtt
     3001 cgcgcaccga agcgagtagg tcgagggtct cgttgaccgg catctcctcg ggcgtggtga
     3061 cggtgatcag gccggtgcgc tgcgggtctt cgaggatgtc gagcatccac gcggtctgat
     3121 ccttgaccag gccgacctgc accagttcgc cgatcacccg cggtgcaccg atctgggcca
     3181 cgatgtggcc gctcgcttcg gcatcgacga cgacgaggtc gtaatggcgc tcgcggacct
     3241 cgtagcacag cttgccgacg gcaaggatct ccttgacccc cggtgccgcg tcggcgacga
     3301 agtcgaaggt gcgcgccaac gggccgatac gccccacgaa ggggatgcgc acgaacagcc
     3361 gcaggtactc gcgcagcgag tcctcggtgt tcatcgccat gccgaacagg ccggtgtcga
     3421 tccggcgtgg ctggaacgtg aacggcgagc agtcgaacgc ggtcgccaac gaccccttcg
     3481 cgtccatctc gcacaccaac gtcgagcgcc cgtgctgggc ggcaagttgg gcgatcgccg
     3541 cggcgaccgt ggtcttgccg acgccgccct tgccggtgac gaacagcagc tgccgatcga
     3601 gcagattcat acggcaacca ggttcacgcg gcgagcggac tcggatcagc cgaagccgac
     3661 gcggcggacg tggtcggccg aaccgatgtc gacgtaggcg atcttggccg cactgacacc
     3721 gatctcgcga cccttcttgt cggtgaccca caacacgtcg atcgcacccg tcagcgcgtg
     3781 ggttcgacca cgtcgatccg gctctcgatg ccgctgttct cgtcggcgag ttcgacggtg
     3841 agctctcggg cgatatcggt gacaccgatg cggagatcca cggcagtcct ttcctgtcgc
     3901 gaccccaacg gccgcagcac tcgcccgcca tcgtacggcg caccgtaggc tcgcgggcat
     3961 gaccgcagcg gcaactccgc cagcagccgc gacaccggcg accgcgacat cggcgaacgc
     4021 gacaccgcgc cgcccgcgcc tgcgtgcggc cgatcggcgc actctcatct tggaagccgc
     4081 ccgccgggct ttttcggcca gcggcgacgt acgcggcacg acggtgaagc agatcgccga
     4141 agaggccggg atcagcgagg ggatccttta ccgccacttc gacagcaagg acgagttgtt
     4201 cgtccaggcc gcggtggaac cgctgaccgc cgcgatgcag acgatcatcg actccgtcag
     4261 cggactcgac ctgaccaagt tcggccccga gttgcacgac agcagcgtgc agtactggca
     4321 ggagacgatc gatgccgtca ccgagttggt gccnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4381 nnnnnnctat gcggtgccgt tctatcgcga cgtgctcgcg ccagcgctga agcagatgcg
     4441 cgattcgtgg gatgcgatct acatccgcga gttcggcgag ccgttccccg accgcgagaa
     4501 cgccacgtcg cacatcggca cgatcgtgat gctggcgctc gaacgccgcc tcgcggccac
     4561 gccgcgcacg tcgtacgaca tggccctcca actggtcgcg accgaggagt tccacctccg
     4621 ctccgcgatc gcggcggtat ctcgccgacg aacaccaggc accaaggcac caagcaccaa
     4681 gacgaccggg accaaaccga cc
//
LOCUS       DAORNF010000187         4665 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_13712_length_4665_cov_1.623210, whole genome shotgun sequence.
ACCESSION   DAORNF010000187 DAORNF010000000
VERSION     DAORNF010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4665
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13712_length_4665_cov_1.623210"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..793
                     /locus_tag="PLV68_08695"
     CDS             <1..793
                     /locus_tag="PLV68_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01364.1"
                     /translation="LGQIYTPPWKTEDGLTLKWEPDADTTDTLEPPTADAAIVATDTV
                     DADTVGADTVGADTADAAAAPDTGADAALVATAAMPVLPARTPAPNKPGSGMVIEDGV
                     ALTGGDRDDDPSATATPAKPPRRRRRFGGGMFLFVTAIAAIIAITFAVIAVHARSGYF
                     VGFHDDTVVIYKGQRDAVLWFHPTVHTPSTKTRSQLAPEVVTAIEKKPTFPSADAAAE
                     FIQDLVPTTPGDPDTDGTGDSIPTSTTAAPSLTPLSTTTTEPTGG"
     gene            793..>1147
                     /locus_tag="PLV68_08700"
     CDS             793..>1147
                     /locus_tag="PLV68_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HRE01365.1"
                     /translation="MHPLSRRGTELGLVIMAGTITAAAYVLASLGKNAEIPPVIVPFF
                     VILLGLLLTAHIATRFLARGADATLVPLAMMLHGLGYVMITRLNDRLAGLQTTWTFVA
                     IAAYIGTLLVVQRAPD"
     assembly_gap    1148..1316
                     /estimated_length=169
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1392..2222
                     /locus_tag="PLV68_08705"
     CDS             1392..2222
                     /locus_tag="PLV68_08705"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HRE01366.1"
                     /translation="MPQPRYLMPLLAAWAFAVVVMVGQKDLGSSLLFFTLFVVMVWVA
                     TERIGFLILGLLLFAGAAYLSYRLFGHVKTRVDIWIDPWSRYSDGRGDRGRQIVEAAF
                     AIGDGGLTGAGLGQGLPTRVPAVSTDFIFAAIGEELGLLGSSLVLMGFLLIVGAGLRI
                     AIRAERPFEKLLATGLTALLGVQAFIIIGGVIRVVPLTGITLPFVSYGGSSLVANYII
                     LALLMRVSDSTSRRLGELPDEPTINERFAAWRLRRSLRAQDKRAAKAGARAAGQAPTR
                     "
     gene            2219..>3311
                     /locus_tag="PLV68_08710"
     CDS             2219..>3311
                     /locus_tag="PLV68_08710"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRE01367.1"
                     /translation="MNKQIRRLAIALMVCYLALFVQLNRLQVLEREELATNTNNNRDE
                     VRDFDKPRGQIVSADGVVLAQSVLATDSKYTYQRDYPTGDLFANVTGYLTFAYGSTKV
                     ESKYSDVLTGQTSEQQLRNLTNLFSRESNTGSVHLTMRADLQEVAKDALGEREGSVVV
                     IDTHTGAVVAMYSYPSYDPNLVAVHDNNLARDRIEFLNNYPGKPTLANAYQERYMPGS
                     TFKVITTAIGLENGVIDFDSTWPNEREWTPPQTDNPIQNYNGTTCGGDLREVFRRSCN
                     IPFAKLAVEIGPERMVAGTQAWGIGERIPFDLSGAVASNFGTVEELHNNLARLAIRGF
                     GQDDDLMVPLHMAMVAATVANGGQMMKPYV"
     assembly_gap    3312..3327
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3328..3680
                     /locus_tag="PLV68_08715"
     CDS             <3328..3680
                     /locus_tag="PLV68_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRE01368.1"
                     /translation="RTVPSVWKTPISPETALKLTALMIGVVQNGTATCCLQLANGVQA
                     AAKTGTAQLNATGQPQRSHAWITAFAPAEAPRYAIAVVLKGTTAEISAGTGGVLAGPI
                     AKTVLDAALAIPES"
     gene            3790..>4336
                     /locus_tag="PLV68_08720"
     CDS             3790..>4336
                     /locus_tag="PLV68_08720"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HRE01369.1"
                     /translation="MTNHGDRTVLNDRYEIQQRIGRGGMADVLLARDLLLDRPVAIKV
                     LFAEYATDPAFVERFRREAQAAANLTHPNIVGVYDWGKYGGTYFIAMEYVQGRTLADI
                     IRANGRISAVQAAEVASEVAAALAFAHRNGVVHRDIKPANILIGSSGQVKVADFGIAR
                     ALNATADSNLTQVGLVMGTATY"
     assembly_gap    4337..4374
                     /estimated_length=38
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4375..>4665
                     /locus_tag="PLV68_08725"
     CDS             <4375..>4665
                     /locus_tag="PLV68_08725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01370.1"
                     /translation="DLYSLGVVMYEMVAGRPPFTGENPVAIAYKQVHESPQPLNQIMP
                     GIPRPYEAIVAKLLAKNPTVRYVDGEALRDDLRRFRNGEPVRALGGAAAGTA"
BASE COUNT          758 a         1636 c         1384 g          664 t
ORIGIN      
        1 gctcggtcag atctacacgc cgccctggaa gaccgaggac ggcctcacgc tcaagtggga
       61 gccggacgcc gacaccaccg acacgctcga gccgccaacg gccgatgcag ccatcgtcgc
      121 aaccgacacc gttgacgccg acacagttgg cgccgacacc gttggcgccg acaccgctga
      181 cgccgctgcg gcgccagaca caggggccga tgctgcactg gttgccacgg cggcgatgcc
      241 cgtgttgccc gcacgcacac ccgcaccgaa caagcccggt tcgggcatgg tgatcgagga
      301 cggcgtcgca ctcaccggcg gtgaccgtga cgacgacccg tcagcgaccg ccacacccgc
      361 caagccgccg cggcgacggc ggcgcttcgg cggcggcatg ttcctgttcg tcaccgcgat
      421 cgcagcgatc atcgcgatca ccttcgccgt catcgccgta cacgcccgca gcgggtactt
      481 cgtcggcttc cacgacgaca ccgtggtcat ctacaagggc cagcgcgacg cggtgctgtg
      541 gttccacccc accgtgcaca cacccagcac caagacccgc tcccaactcg cgcccgaagt
      601 cgtcacggcg atcgagaaga agcccacgtt tccatcggcc gacgccgcgg cggagttcat
      661 ccaggacctc gtaccgacca caccaggtga ccccgacacc gacggcaccg gcgactccat
      721 ccccaccagc accacggccg cgccttcact cacacctctc agcacgacca cgaccgaacc
      781 aacggggggc tgatgcaccc gctctcgcgg cgcggcaccg agctcggctt ggtgatcatg
      841 gcgggcacga tcaccgcggc cgcctacgtg ctggcctcgc tgggtaagaa cgccgagatc
      901 ccgccggtca tcgtgccgtt cttcgtcatc ctgctcggcc tgttgctgac cgcgcacatc
      961 gccacccggt tcctcgcgcg cggcgccgac gccacgctgg tgccgctggc gatgatgctg
     1021 cacggcctgg ggtacgtgat gatcacgcgc ctcaacgacc gccttgccgg gttgcagacc
     1081 acgtggacgt tcgtcgcgat cgcggcctac atcgggacgc tgctggtggt gcagcgcgcc
     1141 cccgaccnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngccg
     1321 gcatcttctc cccgtgaacg gggagaagag agctgatcgc cgccagcacc tggaagatcg
     1381 gcccgctgcg gctgccccaa ccgcgctacc tcatgccgct gctggcggcg tgggccttcg
     1441 ccgtggtcgt gatggtcggt cagaaggacc tcggctcgtc gctgctgttc ttcaccctgt
     1501 tcgtggtgat ggtctgggtg gccaccgagc ggatcggctt cctcatcctc ggcctgctgc
     1561 tgttcgcggg tgcggcatac ctgtcgtacc ggttgttcgg tcacgtcaag acccgcgtcg
     1621 acatctggat cgatccgtgg agccgctact ccgacggccg cggcgaccgt ggccgccaga
     1681 tcgtcgaggc cgccttcgcg atcggcgacg gcggcctcac cggcgccggt ctgggacagg
     1741 gactcccgac tcgcgtgccc gccgtgtcga ccgacttcat cttcgccgcc atcggtgaag
     1801 agctcggatt gctcggttcg tcactggtgc tgatggggtt cctgctcatc gtcggcgcgg
     1861 gactgcgcat cgcgatccgt gccgagcgcc cgttcgaaaa gctgctcgcc accggcctga
     1921 ccgcgctgtt gggcgtgcag gcgttcatca tcatcggcgg cgtcatccgg gtggtcccgc
     1981 tcaccggcat caccctgccg ttcgtcagct acggcggctc gtcgctggtg gccaactaca
     2041 tcatcctcgc cttgctgatg cgggtcagcg acagcacgtc ccggcgtctg ggtgagctgc
     2101 ccgacgagcc gaccatcaac gaacgcttcg ccgcatggcg tttgcgccgg tcgttgcgtg
     2161 cgcaagacaa gcgcgccgcc aaggccgggg ctcgcgccgc gggtcaggcg ccgacgcgat
     2221 gaacaagcag atccgccggc tcgcgatcgc actgatggtg tgctacctcg ccctgttcgt
     2281 gcagctcaac cggttgcagg tgctcgagcg cgaagagttg gcgaccaaca ccaacaacaa
     2341 ccgcgacgag gtgcgtgact tcgacaagcc ccgcggccag atcgtgtcgg ccgacggcgt
     2401 ggtgctggct cagtcggtgc tcgccaccga ctcgaagtac acgtaccaac gggactaccc
     2461 caccggcgat ctgttcgcca acgtcaccgg ttatctcacc ttcgcctacg gctcgaccaa
     2521 ggtcgagagc aagtacagcg acgtgctcac cggccaaacc agcgaacagc agttgcgcaa
     2581 cctgaccaac ctgttcagcc gcgagtccaa caccggctcg gtccacctga cgatgcgggc
     2641 cgatctgcaa gaagtggcca aggacgcgct cggcgagcgg gaaggcagcg tggtggtgat
     2701 cgacacccac acgggtgcgg tcgtggcgat gtacagctac cccagctacg accccaacct
     2761 ggtcgcggtg cacgacaaca acctggcccg cgaccggatc gagttcctca acaactaccc
     2821 cggcaagccg acgctggcca acgcctacca agagcgctac atgcccggct cgacgttcaa
     2881 ggtgatcacc accgcgatcg ggttggagaa cggcgtgatc gacttcgact cgacgtggcc
     2941 gaacgagcgc gagtggacac ctccccagac cgacaacccg atccagaact acaacggcac
     3001 cacctgcggt ggcgatctgc gcgaggtgtt ccggcgcagc tgcaacatcc ccttcgccaa
     3061 gctcgctgtc gagatcggtc cggagcggat ggtcgcgggc acccaggcct ggggcatcgg
     3121 cgagcggatc cccttcgacc tgtcgggcgc ggtggcctcc aacttcggca cggtcgagga
     3181 gttgcacaac aacctcgccc gcctcgccat ccgcggcttc ggtcaggacg acgacctgat
     3241 ggtgccgctg cacatggcga tggtggccgc caccgtcgcc aacggcgggc agatgatgaa
     3301 gccctacgtg gnnnnnnnnn nnnnnnnacc gcaccgttcc ttccgtgtgg aagacaccga
     3361 tctcacccga gacggcgctg aagctgaccg cgttgatgat cggcgtggtg cagaacggca
     3421 ccgcgacctg ctgcctgcaa ctggccaacg gcgtgcaggc cgcggccaag accggcaccg
     3481 cccaactcaa cgccaccggg cagccgcagc ggtcgcacgc atggatcacc gcgttcgctc
     3541 cggccgaggc ccctcgctat gcgatcgcgg tcgtgctcaa gggcaccacg gccgagatca
     3601 gcgccggaac cggtggtgtg ctggccggac cgatcgccaa gaccgtcctc gacgccgccc
     3661 tcgcgattcc ggagtcgtga tgcacagcgg tcgtagccag agcgccccac aaccgggccg
     3721 caagccactc cacaacgcca gaggggtagc ctcgctggcg ccgctcgaac cacgggacac
     3781 gaaccaacag tgacgaacca cggagaccgc acggtcctca acgaccgcta cgagatccag
     3841 cagcgcatcg ggcgcggcgg gatggccgac gtgctcctcg cgcgcgacct gctgctcgac
     3901 cgccccgtgg cgatcaaagt gctgttcgcc gagtacgcca ccgatcccgc gttcgtcgag
     3961 cggttccgcc gcgaagccca ggccgcggcc aacctgaccc accccaacat cgtcggcgtc
     4021 tacgactggg gcaagtacgg cggcacctac ttcatcgcga tggagtacgt ccagggccgc
     4081 accctggccg acatcatccg cgccaacggc cgcatctcgg cggtgcaggc cgccgaggtc
     4141 gccagcgagg tcgcggccgc gctggccttc gcccaccgca acggcgtggt gcaccgcgac
     4201 atcaagcccg ccaacatcct catcggctcc agcggtcagg tgaaggtggc cgacttcggt
     4261 attgcccgcg ccctcaacgc cacggccgac agcaacctca cccaggtcgg cctggtgatg
     4321 ggcacagcca cctactnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngcgacc
     4381 tgtactcgct cggcgtcgtg atgtacgaga tggtggccgg tcgcccgccg ttcaccggtg
     4441 agaaccctgt cgccatcgcg tacaagcagg ttcacgagtc gccccagccg ctcaaccaga
     4501 tcatgcccgg catccctcgc ccctacgagg cgatcgtcgc caagttgctc gccaagaacc
     4561 ccaccgtgcg ctacgtcgac ggcgaagcac tgcgcgacga cctgcgccgg ttccgcaacg
     4621 gcgaaccggt gcgcgccctc gggggcgcgg cggccggcac cgcca
//
LOCUS       DAORNF010000188         4654 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_13756_length_4654_cov_1.832355, whole genome shotgun sequence.
ACCESSION   DAORNF010000188 DAORNF010000000
VERSION     DAORNF010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4654
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13756_length_4654_cov_1.832355"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            89..>424
                     /locus_tag="PLV68_08730"
     CDS             89..>424
                     /locus_tag="PLV68_08730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01371.1"
                     /translation="MTPPPVPQAPIPVSYADPPPTLPLRPLTYYPFTTMPDQPQIAIG
                     DNGVVVLNEDGTRAATIDWDGGHSSTVTLDAPISQPVMGPDDVLYGLTNIGPGPADFG
                     MVAIPLRGPR"
     assembly_gap    425..565
                     /estimated_length=141
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <566..2149
                     /locus_tag="PLV68_08735"
     CDS             <566..2149
                     /locus_tag="PLV68_08735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01372.1"
                     /translation="GEPLTAPIEAPAVATIDSVGLVTSGGVAHWQLAIERDPACCRSV
                     LDASPPVAAPDGKSVHYWTEIGPPRYPEADYSESTLTAFADLHADGTGTWYSVPTGWH
                     IAASDLGTTILWRLDDSDDIQLALFSPLSRAPMNREAWMLQTLGVDASCPEQCSAWGV
                     LADGALVRFDRATAVVTALGPPSWTLDISATVGTNGDAWLVAIGPADVGYFTVPSRVN
                     PGEADDLVAIALTGNEAGHEVGRRANVFGTDSRLVPTVEGIVSVGCCGLPSIRPPADA
                     ELVMAWVDATGAVTTDQGARLSMEFVTGGVRVVRLDGTAQREWTIATPINLAYRGVPP
                     MVALDDGGVLIFLEDSFGMAGNEPARLLHLSTDGDVADVMYQPVGRPFLLTPSGSAVV
                     NLGNQYALISPEDAALHAELSNTLADGHPSDERVFAALTGRQFTDLAAIGDAAFTVLK
                     PSFPEDIIAMGVVKRVHIFATGPIGVVVTVPQPDDSVVAMVWFVRAEPVGTGYRVIDV
                     RVADRCRTGNLRAPDELCI"
     gene            complement(2146..>2455)
                     /locus_tag="PLV68_08740"
     CDS             complement(2146..>2455)
                     /locus_tag="PLV68_08740"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRE01373.1"
                     /translation="ALAEGRTLSWDDAIAFARRSRGERKRPVLGWDALTPTEREVVEA
                     IADGLTNPQVAARLLMGRETVKSHVSSVYTKLAVTNRVQLVALVVARPPVTGPQRLEG
                     "
     assembly_gap    2456..2467
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2468..2987)
                     /locus_tag="PLV68_08745"
     CDS             complement(<2468..2987)
                     /locus_tag="PLV68_08745"
                     /inference="COORDINATES: protein motif:HMM:NF017539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NACHT domain-containing protein"
                     /protein_id="HRE01374.1"
                     /translation="MTDAATPPPCAHNLPAELSAFVGRTEEIGTVARLVASNRLVTLT
                     GSGGAGKTRLAQRVAASVADEFADGVWWIELASTVAAEQIATTILGALGEPIGNSDPL
                     RLLVLRLATSHTLLVVDNCEHLVEDVAEVLHAVLSTCPSVSVLATSRTALNLPGEVSW
                     RVPPLASFRRRAL"
     gene            complement(2984..3601)
                     /locus_tag="PLV68_08750"
     CDS             complement(2984..3601)
                     /locus_tag="PLV68_08750"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRE01375.1"
                     /translation="MGFYNTRIVPRIINCGCGTPAFGETRTRVLGGLTGEVLEIGFGT
                     GHNLAHYPPSVSRIHAVEPSARSIELAAERIAASSAEVVVSGDDAQQLALADESFATV
                     VSTWSLCTIPDPDAAMAEIVRVLKPGGRFHFVEHGKSPDAKVARRQRRFEPMWRRMSG
                     NCHITRNIDDIVRSSGLEMVSLNTFAHPKDFKVMNWTFEGVAVRR"
     gene            3700..>4654
                     /locus_tag="PLV68_08755"
     CDS             3700..>4654
                     /locus_tag="PLV68_08755"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HRE01376.1"
                     /translation="MDAPSLVEIAEQVRTGRRSARSVVTEALATIQERNDRCNAFVAI
                     DAARAIADAEALDRRVLHDGPAPPLAGVPLAVKDTEDAAGYRTTFGSQLHASSPVSAT
                     DSVLVARLKAAGCIVVGRTNTPEFAARGVTDNLVFGATRNPHDRDRTAGGSSGGSAAA
                     VASGMVPLATGSDGGGSIRIPASACGIPGFKPSLGRIPDGDPRPFDWHDLSSKGVFTR
                     TVTELLAVLDVIVGPHPSDLRSLPAPFSISVARQPLPRRVGFSATLGYAEIDPAIERA
                     CRSVLERLAADGVDVVEIPRVFDADPMPAWLTLVTNYLSRSV"
BASE COUNT          703 a         1613 c         1478 g          707 t
ORIGIN      
        1 tgaggtcacg ctgccgtcga ccccccaacc gccgtcgagc ccgcccgagt ccgtcccgga
       61 gtccacgctc gcgacaccgg tcgcgccggt gacaccgccg cctgtgcccc aggcaccgat
      121 cccggtcagc tacgccgacc cgccgccgac actgccgctg cgaccgctca cctactaccc
      181 gttcacaacc atgcccgacc aaccgcagat cgccatcggc gacaacggag tcgtcgtgtt
      241 gaatgaggac ggcacccggg ctgcgacgat cgactgggac ggcggccact cctcgaccgt
      301 cacgctcgac gccccgatct cgcagcccgt catgggaccg gacgacgtgc tctacgggct
      361 gaccaacatc ggccccggcc cagccgactt cggtatggtc gcgatcccgc tccgcggtcc
      421 tcgcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      541 nnnnnnnnnn nnnnnnnnnn nnnnnggcga gccgctgacc gccccgatcg aggcacccgc
      601 ggtggcgacg atcgacagcg tgggcctggt cacgtctgga ggcgtcgccc actggcagtt
      661 ggcgatcgag cgcgacccgg cgtgttgccg ctcggtcctc gacgccagcc caccggtggc
      721 cgcgcccgat ggcaagtcgg tgcactactg gaccgagatc ggcccacccc gctacccaga
      781 ggccgactac agcgagtcga cgttgaccgc ctttgccgac ctccacgccg acggcacggg
      841 cacgtggtac tcggtcccga ccggctggca catcgccgcc agcgacctcg gcacgaccat
      901 cctctggcgc ctcgacgact ccgacgacat ccaactcgcc ctgttctccc cgctgtcgag
      961 ggcaccgatg aaccgggaag cgtggatgct gcagacgctc ggagtcgacg cgtcgtgtcc
     1021 ggagcagtgc tcggcctggg gtgtgctcgc agacggcgcg ctggtgcgtt tcgaccgggc
     1081 aacagccgtg gtgaccgcgc tcggtccacc atcgtggaca ctcgacatat cggcgacggt
     1141 cggcaccaac ggcgatgcct ggctcgtcgc catcggcccc gctgacgtcg gctacttcac
     1201 ggtcccgtcg cgcgtgaatc caggggaagc ggatgacctg gtggcgatcg cgctcaccgg
     1261 caacgaggcg ggacacgagg ttggtcgacg tgccaacgtg ttcggcacgg actcgcgact
     1321 ggtcccgacc gtcgagggca tcgtgagcgt ggggtgttgt ggcctgccgt ccatccgtcc
     1381 tcccgccgac gccgaactgg tcatggcatg ggtcgatgcg acaggcgcgg tcacgaccga
     1441 ccaaggcgca cggctgtcga tggagttcgt cacaggcggt gttcgtgtgg tgcgtttgga
     1501 cggaaccgca cagcgagagt ggaccatcgc aacgccgatc aacctcgcct atcgcggggt
     1561 gccgcccatg gtcgcgctgg acgacggcgg cgtcctgatc ttcctcgaag acagcttcgg
     1621 gatggcaggc aacgaacccg cccgtctgct gcatctgagc accgatggcg atgtggccga
     1681 cgtgatgtac cagcccgttg gccgtccgtt cctgctgacg ccgagcggat cggcggtggt
     1741 caacctgggc aaccagtatg cgctgatcag ccccgaagat gccgccctcc acgccgagtt
     1801 gtcgaacacc ctcgccgatg gacacccctc cgacgagagg gtgttcgcgg cactgaccgg
     1861 gcggcagttc accgatctcg cggcgatcgg tgacgcggcg ttcaccgtgc tgaagccgag
     1921 ctttccggag gacatcatcg cgatgggcgt cgtcaagagg gtgcacatct tcgcgaccgg
     1981 tccgatcggc gtggtcgtca cagtgccgca accagacgac tcggtggtgg cgatggtgtg
     2041 gttcgtgcgg gccgaaccgg tcggcaccgg gtatcgcgtc attgacgtcc gggtcgccga
     2101 ccgctgccgc accggcaacc tgcgcgcccc cgacgagctg tgcatctaac cctcaagcct
     2161 ctgtggccca gtcacgggcg gccgggcgac caccagcgcg accaactgca cacggttcgt
     2221 caccgccagc ttggtgtaca cgctcgacac gtgcgacttg accgtctctc gacccatcag
     2281 cagtctggca gcaacctgcg ggttggtgag gccatccgca atcgcctcga ccacctcccg
     2341 ctccgtcggg gtcaacgcat cccagccgag caccggacgc ttgcgttcgc cccgagaccg
     2401 gcgggcaaac gcgatggcgt cgtcccacga cagggttcgc ccctcggcca acgcannnnn
     2461 nnnnnnntca gcgctcgccg tcggaagctc gcaagcggcg ggaccctcca tgagacctcg
     2521 ccgggaaggt tgagtgccgt gcggctcgtg gccaacaccg acaccgacgg gcacgtcgag
     2581 agcacggcgt gaagaacctc cgccacgtcc tcgacaaggt gctcgcagtt gtcgaccacc
     2641 agcagcgtgt ggctcgtggc gagtcgcagg accaacaggc gcaacgggtc gctgttgccg
     2701 atcggctcac cgagcgcgcc aaggatcgtc gtcgcaatct gctcggccgc gacagtgctc
     2761 gccagttcga tccaccacac accgtccgcg aactcgtccg ccacgctcgc cgcgacccgc
     2821 tgcgccaacc gggtcttgcc cgcacctccg cttcccgtca gcgtgaccag ccggttcgat
     2881 gcgacgaggc gcgcgaccgt gccgatctcc tccgtgcgcc ccacgaacgc cgacagttcg
     2941 gcgggcaggt tgtgcgcaca cgggggcggc gtcgcggcgt cggtcatcga cgcaccgcaa
     3001 cgccttcgaa ggtccagttc atcaccttga agtccttggg atgagcaaag gtgttcagcg
     3061 acaccatctc cagcccggaa ctgcggacga tgtcgtcgat gttgcgcgtg atgtggcagt
     3121 tgcccgacat gcgcctccac atcggttcga atcgccgttg gcggcgggcg accttggcat
     3181 ccggcgactt gccgtgctcg acgaagtgaa accgtccacc gggcttgagc acgcgcacga
     3241 tttctgccat cgctgcgtcc gggtcaggga tggtgcacag cgaccaggtc gacacgaccg
     3301 tcgcgaagct ctcgtcggcg agggcgagtt gctgcgcgtc gtcaccggac acgacgacct
     3361 ccgccgacga cgcagcgatc cgctcggccg cgagctcgat cgagcgagcc gacggctcga
     3421 cggcatgtat ccgcgacacc gagggcggat agtgggccag gttgtgtccg gtgccgaagc
     3481 cgatctcgag tacctccccc gtgaggccac cgagaacgcg agtgcgtgtc tcgccaaacg
     3541 ctggcgtgcc acagccacag ttgatgatgc ggggaacgat ccgtgtgttg tagaagccca
     3601 tctctccatc ctcgatcggt cagtacatga tgacctcccc cacctggggg aaccgcgagc
     3661 ggtgagacgt cgggccaccg gtgtggcgag aatgggggga tggatgcgcc gtcgttggtg
     3721 gagatcgccg agcaggttcg cacggggcgg cggagcgcgc gcagcgtggt gaccgaggcg
     3781 ttggcgacga ttcaggagcg caacgaccgg tgcaacgcgt tcgtcgccat cgacgcggct
     3841 cgggcgatcg ccgacgccga agcgctcgat cgccgggtgt tgcacgacgg tccggcaccg
     3901 ccgttggccg gtgtgccgtt ggcggtcaag gacaccgagg acgcggccgg ataccgcacc
     3961 acgttcggct cgcagctcca tgcgtcgagc ccagtgtcgg cgaccgactc ggtgttggtc
     4021 gcccgactga aagcggccgg gtgcatcgtc gtcgggcgca cgaacacgcc ggagttcgcg
     4081 gcgcgcggcg tcaccgacaa cctcgtcttc ggcgcgaccc gcaaccccca cgaccgtgac
     4141 cgcaccgccg gtggatcctc gggcggctcg gctgccgcgg tcgcatccgg catggtgccg
     4201 ctggcgacgg gatcggacgg tggcggctcg atccgtatcc ccgcctcggc ctgcggcatc
     4261 cctggcttca agccgtcgct cgggcgcatc cccgacggcg acccgcgccc gttcgactgg
     4321 cacgacctca gctccaaggg cgtgttcacc cgcacggtca ccgaactgct cgccgtgctc
     4381 gacgtgatcg tcggcccgca tcccagcgac ctccgctcgc tgcccgcgcc gttcagcatc
     4441 tccgtcgccc gccagccgtt gccgcgacgg gtcggcttca gcgccacgct cggctacgcc
     4501 gagatcgacc cggcgatcga gcgcgcttgc cgctctgtgc tggagcgtct cgccgccgac
     4561 ggcgtcgatg tggtcgagat tccccgtgtg ttcgatgccg acccgatgcc cgcctggctg
     4621 accctggtga ccaactacct cagtcgctca gtcg
//
LOCUS       DAORNF010000189         4651 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_13771_length_4651_cov_1.640339, whole genome shotgun sequence.
ACCESSION   DAORNF010000189 DAORNF010000000
VERSION     DAORNF010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4651
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13771_length_4651_cov_1.640339"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="PLV68_08760"
     CDS             <1..247
                     /locus_tag="PLV68_08760"
                     /inference="COORDINATES: protein motif:HMM:NF037521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zincin-like metallopeptidase domain-containing
                     protein"
                     /protein_id="HRE01377.1"
                     /translation="SYAAEELVAELAAAMWCAQHGVSGVTRPDHAAYLAGWLRILRTD
                     TRALVTASSKAQAAVDHLNTLAGHTNNTSDQNEETAA"
     gene            244..528
                     /locus_tag="PLV68_08765"
     CDS             244..528
                     /locus_tag="PLV68_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01378.1"
                     /translation="MNRPPYRRADEIAAYTYRAAIHCPPCLIETMIATRDASPAARDM
                     PAEDVLDQCAGASTIDRDDEHSYDSDEFPKVVFLDQLPADATCDECGTEL"
     assembly_gap    808..848
                     /estimated_length=41
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<849..1054)
                     /locus_tag="PLV68_08770"
     CDS             complement(<849..1054)
                     /locus_tag="PLV68_08770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01379.1"
                     /translation="MPEQLLRVLVGSARVGVRALEGEPLDGVVAIDDAVPANRLTGPV
                     CAELDDVTTGEPAGNDPTSEFRVG"
     assembly_gap    1116..1160
                     /estimated_length=45
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1476..1514
                     /estimated_length=39
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1515..1940)
                     /locus_tag="PLV68_08775"
     CDS             complement(<1515..1940)
                     /locus_tag="PLV68_08775"
                     /inference="COORDINATES: protein motif:HMM:NF012616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine N-6-methyltransferase family
                     protein"
                     /protein_id="HRE01380.1"
                     /translation="MPRSSHGGRHELGQNFLHHRPTIERFATLVEQTVGPILEIGAGD
                     GALTRAIVQLGRPVTAIDIDEHRVERLRRSTPAATTVHGDALRYPLKTPVIVGNVPFH
                     LTTPILRRLLSSGTWTNAVLLTQWEVARKRAGVGGSTMMT"
     assembly_gap    2614..2661
                     /estimated_length=48
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2662..>4651
                     /gene="mobF"
                     /locus_tag="PLV68_08780"
     CDS             <2662..>4651
                     /gene="mobF"
                     /locus_tag="PLV68_08780"
                     /inference="COORDINATES: protein motif:HMM:NF020332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobF family relaxase"
                     /protein_id="HRE01381.1"
                     /translation="TIRPHPRRVPGFDLTFKVPKSVSVLYAVSDDPGVQGAIVEAGEA
                     AVRDALGWIEREAIHIRRGTGNERFLDSLAARDPDAADQARIRSLSARGVVAAVFRHR
                     TSRAGDPLLHWHTLIPNLAEGPDGRWTAFVHPDLYRSVRAAGEVFQTVLRHELSQRLG
                     IEWRPGRHVPEIAGVPQGLCDRFSKRSHDIEAWLEATGTPNNRQGRQQAVLATRRAKP
                     EVESVRFDAAWKAEALAAGWGPESADALMATLTPDPERDGGRWLDGDRLVASDEWITR
                     VLAELTAEDSTFTRPDLVQAAAGALGEGATIRTVERLVAHALASPRTIPIDAHTGARR
                     WTSADLLDIEARFLAALDPGNECAPVDAAIVGAVIGTRARLGDDQAGAVRQIAGSNGA
                     VSVLVGPAGTGKTFTLDTIREVYESAGFRVVGAAPSARAAMELEAGAGVVSSTLHRLA
                     GEWSRGYDVPDNRTLLIVDEAGMAGVRDLESLVTATVAAGGRVVLAGDHRQLPEVTAG
                     GGFAAAVQGAATVAELSVNRRQVAEWEQSALTELRTGHVATAVGAYREHGRVVVADDR
                     AGMLAAAVDRWFDANRAGRSGVLLAGTNETVDALNRAVRDRLADTDGVGPVLGTFNGR
                     EFRAGERVVLRRNSYRQQTMHGSATDVLNGLTGTAVAVD"
BASE COUNT          706 a         1517 c         1566 g          689 t
ORIGIN      
        1 cagctacgcc gccgaggaac tcgtcgccga gctcgccgcc gcgatgtggt gcgcccagca
       61 cggcgtcagc ggcgtcactc gacccgacca cgccgcctac ctcgccggct ggctccgcat
      121 cctgcgcacc gacacccgcg ccctggtcac cgcgtcaagc aaagcccaag ccgccgtcga
      181 ccacctcaac accctcgccg gccacaccaa caacaccagc gaccagaacg aggagaccgc
      241 agcatgaacc gaccgcccta ccgccgagcc gatgagatcg ccgcctacac ctaccgcgcc
      301 gcgatccact gcccgccatg cctcatcgag acgatgatcg caacccgcga cgcatcgccg
      361 gcagcccgcg acatgccagc cgaggacgtg ctcgaccagt gcgccggagc gagcacgatc
      421 gaccgcgacg acgagcacag ctacgacagc gacgagttcc ccaaggtcgt gttcctcgac
      481 caactccccg ccgacgcgac ctgcgacgag tgcggaaccg aactctgacc agccaggcgg
      541 cgcgtcgacc ggggcatcgc gcccggctgt cgccggtctg ccgtggccgc cctccgggcg
      601 gcgtgccgtc gggccgtttc taacagttcc gaacttcggc ctgtcgtacg gtcgcgctcg
      661 atgcacccgt accaagcctt cacaccgcac ggcggctacg actggtcgcg gttcggacgc
      721 catcgcctcc gtgcggctgg cggggtgttg atcgactctc cgctcggccc ggccgggcat
      781 ggatggttgg ggacactgtg gctcgccnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      841 nnnnnnnnca cccgacccgg aactcggagg ttggatcgtt cccggccggc tcgccggtgg
      901 tgacgtcatc gagttcggcg cagaccggac cggtcaagcg gttcgctggt acggcatcgt
      961 cgatagctac gacgccgtcg agtggctcac cctccagggc ccgtaccccg acccgtgcgc
     1021 tgcccaccag cacgcgcagc agctgctcgg gcacatgcgc taccgatcgc cgaatcgggc
     1081 gcatcgcacc cagcgccggt gcagccggac gccatnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn gtccgtggct ggggcgagtg ctgtctttgt ccactcgccg
     1201 acgccgacca gatgcttgcc cattgcgcgg gctcgatgtc gcggggaagg gcacgatggc
     1261 tgatcccagc gtggttgatg gcttggtggg cctgcctgct gttcagtggg gtggcgcgct
     1321 cgaggatccg tgcgagtgtg ccgccgcggc cggtgaagat cgatcgtacg aactgctcgt
     1381 aggcgtgacg gtgttggacc ggtaccaacg gcgcgggtct gcgggtgatg ctgaggatcc
     1441 cgccgtcgac gcttggccgg ggggtgaagc ctgatnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnggtcat catcgtggag ccaccaactc cggcgcgttt gcgggcgacc
     1561 tcccattggg tgagcagcac cgcgttcgtc catgtgcccg agctcaacag gcgtcgcagg
     1621 atcggggtgg tgaggtggaa cggcacgttg ccgacgatga ccggcgtctt cagggggtag
     1681 cgcaacgcgt cgccgtggac ggtggtcgcg gctggggttg agcgacgaag acgttcgacg
     1741 cggtgctcgt cgatgtcgat cgcggtgacg gggcggccga gctggacgat cgcgcgggtc
     1801 agtgcgccgt caccggcacc gatttcgagg attgggccga cggtctgctc aacgagcgtc
     1861 gcgaatcgct cgatggtggg tcggtgatgc aggaagtttt ggccgagttc gtgtcggccg
     1921 ccatgggaag atcgtggcac gggtgcgctc cagaacgaag ggtggagcgc ctcgagggca
     1981 gacgaggggg caacgtcgaa gttgcgcggg tgtcgcccat ccgaggcgca aggactctcg
     2041 gagggacgac gcgctggtac gtatcgggtg cgagcgcgcc gtcaggcgcg gcgatcgcga
     2101 atgtcgaaag cgttcgccgc aacgaacgcg ctcttggttg ctcccatggc gagcgaggct
     2161 agcgatgaat cgccatcgac ggacctgcct cggctcccgt ttgcagagtc gggaagcgac
     2221 attcctgtca gaggatcgtc accgacgcgg gtgagatgct tcgaaggcgg cgctcaaacc
     2281 atcgcgcaag ctcctgattc ccggcaccgt cctcgcaaga accgagggac gtagccatga
     2341 gcgtcacaca gacgatcacc acacgtggtg tgcacaggtc ccggcccgtc gggacgtacc
     2401 gagggaccaa cccagggacc ttggatcggt gcgatgggcg ggtgctcagc atcgccaagc
     2461 tccgtgtcgg ccaggaggcc taccaactgt ccggcgtggc tcagtcgctc gacgactact
     2521 acaccggccg cggtgaggcc gacggcgtgt ggatcggcgc cggagcggag cggctcggcc
     2581 tgtccggtga agtcggcgga gacgacctgc gggnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nacgatccgt ccccatccgc gccgggtgcc ggggttcgat
     2701 ctgacgttca aggtgcccaa gtcggtgtcg gtgctctacg ccgtcagcga cgacccgggt
     2761 gtgcaggggg cgatcgtcga agctggcgag gccgcggttc gtgacgcgct cggttggatc
     2821 gaacgcgaag cgatccacat ccgtcgcggc accggcaacg aacggttcct cgactcactc
     2881 gctgcccgtg atcccgacgc cgcggatcag gctcgcatcc gaagcttgag cgcgcgtggt
     2941 gtcgttgccg cggtgttccg tcatcgcacc agccgcgccg gtgatccgct gctgcactgg
     3001 cacaccctga ttccgaatct ggccgagggc cccgacggac gctggacggc gttcgtccat
     3061 ccggacctgt accggtcggt gcgagcagcg ggtgaggtgt tccagaccgt gctgcgacac
     3121 gagctgagcc aacggctcgg gatcgagtgg cgaccggggc gacacgttcc cgagatcgcc
     3181 ggtgtccccc agggcctgtg cgaccggttc agcaagcggt cgcacgacat cgaggcctgg
     3241 ctcgaagcca ccggcacgcc caacaatcgg cagggtcgcc agcaagccgt gctcgccacc
     3301 cggcgcgcca agccagaggt cgaatcggtc cggttcgatg cggcatggaa ggccgaggcg
     3361 cttgccgccg ggtgggggcc tgagtccgcc gatgcgctga tggccacgct cacccccgat
     3421 ccggaacgcg atggaggtcg atggcttgac ggcgaccgcc tggtcgcgtc ggacgagtgg
     3481 attacccggg ttctggccga gctgaccgct gaggactcga cgttcacccg tcccgacctc
     3541 gtgcaagctg ccgccggagc acttggtgag ggcgccacga tccggaccgt cgagcgactc
     3601 gtcgctcacg ccctcgcctc accgaggact atccccatcg acgctcacac cggcgcacga
     3661 cgctggacgt cagcagacct gctcgacatc gaagcccgct tcttggcggc gcttgatccc
     3721 ggcaacgaat gcgctccggt cgacgccgcg atcgtcggtg cggtgatcgg aactcgggcc
     3781 aggctgggcg acgatcaagc tggcgctgtc cgtcagattg ccggcagcaa tggcgcagtt
     3841 tcggtgctcg tgggaccggc tggcacgggc aagaccttca cgctcgacac gatccgcgag
     3901 gtctacgaat cagctggctt ccgtgtggtc ggcgcggcgc cgtccgcccg cgccgcgatg
     3961 gagctcgaag ccggagccgg ggtggtgtcg tcaacgttgc atcgtctcgc cggggagtgg
     4021 tcccgcggct acgacgtacc cgacaaccgg acgctgttga ttgtggatga ggctggcatg
     4081 gccggcgttc gtgacctcga atcgctcgtc accgcgacgg tcgccgccgg cgggcgggtg
     4141 gtgctcgccg gggaccaccg tcagctcccc gaggtcacag caggcggggg gttcgcggcc
     4201 gccgtccagg gtgctgcgac ggtcgccgaa ctcagcgtga accgccggca ggtcgctgaa
     4261 tgggaacagt ccgcgctcac cgaactccgc actggccacg tcgccaccgc agtcggtgcg
     4321 taccgggagc acgggcgggt ggtcgtcgcc gacgaccggg ccggcatgct cgccgcggca
     4381 gtcgaccggt ggttcgacgc gaaccgcgcc gggcgttcgg gtgtgctgct ggccggcacc
     4441 aacgagaccg tcgacgcact caaccgcgcg gtccgcgatc ggctcgccga cacggacggg
     4501 gtcggaccgg tgctcgggac gttcaacggt cgcgagttcc gagccggcga acgcgtcgtg
     4561 ttgcgccgca actcgtaccg ccaacagacc atgcacggca gcgcgacgga tgtcctcaac
     4621 ggactgaccg gcaccgccgt cgcggtcgac g
//
LOCUS       DAORNF010000190         4620 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_13877_length_4620_cov_1.748959, whole genome shotgun sequence.
ACCESSION   DAORNF010000190 DAORNF010000000
VERSION     DAORNF010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4620
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13877_length_4620_cov_1.748959"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>373)
                     /locus_tag="PLV68_08785"
     CDS             complement(<1..>373)
                     /locus_tag="PLV68_08785"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRE01382.1"
                     /translation="KAEYMDSWKTKFIFPAMGMIPIDRSGGSKSTSALDAAEEVLRRG
                     ELFGIFPEGTRSRDGYLYKGHTGAARLALRVGCPIYPVGIIGTAEIQPPDAKFPKLRK
                     TATIKIGRPIKVERYRNKADDH"
     assembly_gap    374..516
                     /estimated_length=143
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    775..950
                     /estimated_length=176
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<951..1796)
                     /locus_tag="PLV68_08790"
     CDS             complement(<951..1796)
                     /locus_tag="PLV68_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HRE01383.1"
                     /translation="MRLYDTARRAVVSFEPGPLVTMYTCGITPYDATHLGHAATFVIY
                     DVLQRRLIDLGHQVKCVRNVTDVDDPLFAKARELGVHYLDLAAGEEARFERDMAALDM
                     LPMHSTPRASSAISDIRGFIGRVLDRGYAYQAGGSVYFDISKFPSFGTVSHLSREEMI
                     EIARERGGNVDDPHKRDPLDFVLWHPSASDEPSWDTMWGPGRPGWHIECSALAVRELG
                     TTIDLHGGGSDLIFPHHECERAQSEAATGETFVRHWLHGPMVSMDGQKMSKSLGNLVF
                     IDKLRE"
     gene            complement(1815..>2285)
                     /locus_tag="PLV68_08795"
     CDS             complement(1815..>2285)
                     /locus_tag="PLV68_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HRE01384.1"
                     /translation="NALVRVGGEFQPLVPPRGSVAAHVQAAVPRCRVVAAFHHLPASE
                     LGHLGEPIESDVLICSDDKTATEVVAEIVAKIPGCRPLDAGELSNATAIEAFTAVLLQ
                     LNIRYKTRVAPKLTGIKDDPRKRAATAASSPAAPTVVAPTTSPSEPAAAGTAQD"
     assembly_gap    2286..2295
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2296..2614)
                     /locus_tag="PLV68_08800"
     CDS             complement(<2296..2614)
                     /locus_tag="PLV68_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HRE01385.1"
                     /translation="MRIGILGGTGPAGSALAARLASVGYTCVIGSRSKYRAMEVCDKL
                     TSRWPEIADRIESADNPGAASCDLVVIATPWDGAATTAQEYEELLRGKVVVINFWASW
                     CGPC"
     gene            2701..3468
                     /gene="fabI"
                     /locus_tag="PLV68_08805"
     CDS             2701..3468
                     /gene="fabI"
                     /locus_tag="PLV68_08805"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HRE01386.1"
                     /translation="MGLLSGKNIVVTGVLTDASLAYGVAKLAQQEGANIVLTGAGRAL
                     SLTERTARKLDTPVPVYEFDVTVAEHVVTVREALAERWGRVDGVLHSIGFAPPSCLGD
                     DFMGAPWDDVSVAMNISTYSLKTLADAFVPLMTAGGSFVGLDFDNSVAWPAYNWMGVA
                     KSALESVNRYLAKELGPKGIRSNLVAAGPIRTMAAKSIPGFAKFEDVWSDRAPLGWDV
                     YDSSAVAKAVVALLSDWFPATTGEIVHVDGGFHAVAV"
     gene            3468..>4620
                     /locus_tag="PLV68_08810"
     CDS             3468..>4620
                     /locus_tag="PLV68_08810"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HRE01387.1"
                     /translation="MNRLATETSPYLRQHADNPVDWYPWGDEAFAAAQARDVPVLLSI
                     GYSACHWCHVMAHECFEDAEVAAVMNEHFVSIKLDREERPDVDAVYMEAIQAMGQRGG
                     WPLTVFLTPDRMPFYGGTYYPKPLFLQLMAKIDEVWHQQRAEVTGNTQALFQALSRSA
                     ATTPTDTFPTHELVNSALQGLGRAFDSAWGGFGTPPKFPSTMNLDLVIRAFGRRPTRD
                     AATVFTTTLDAMASGGMYDHIGGGFARYSVDREWLVPHFEKMLYDQALLARVYLHGWQ
                     VTGNEHWRQVVEETIGYVLTDMTHPLGGFLSAEDADSLGPDGHSHEGAYSTWTPDEVV
                     AVLGPELGRAANEWYGITAEGNFESRSIPNRLAHRGGLIRPPEIDRARTL"
BASE COUNT          681 a         1456 c         1448 g          706 t
ORIGIN      
        1 gtgatcgtcg gccttgttgc gataccgctc gaccttgatc gggcgcccga tcttgatggt
       61 cgccgtcttg cgtaacttgg ggaacttggc atcgggtggt tggatctcgg cggtgccgat
      121 gatgccgacc ggatagatcg ggcaacccac gcgcaacgcc aagcgcgccg cgccggtgtg
      181 ccccttgtac aggtagccgt cgcggctgcg ggtcccctcg gggaagatgc cgaacaactc
      241 accgcggcgc aacacctctt cggccgcgtc gagcgcgctg gtgctcttgg acccgccgct
      301 gcgatcgatg gggatcatgc ccatcgccgg gaagatgaac ttggtcttcc acgagtccat
      361 gtactcggcc ttgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncgac cggacggaag aacgtgcgcg
      541 tcctgcgctg cagtgcacct gcgcccgagt gttcgctgcc tgcggccatg gcgacatagt
      601 aagccccgca ccaggggacg atgtgaacgc agatgcgcga ttgtcggtcg aacgcgtgaa
      661 aatctagaac gacgtcagcg gtcgagcagg atgcccagca gcgcagcggc aacggtgacg
      721 tcgtgacctg ccgcggccgc gtcatcgacg actcgcaccg cgcgggcggt gtcgnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ctcgcgcagc
      961 ttgtcgatga acaccaggtt gcccaggctc ttcgacatct tctggccgtc catcgacacc
     1021 atcggtccgt gcagccaatg gcgcacgaac gtctcccccg tcgccgcctc gctctgggcc
     1081 cgctcgcact cgtggtgagg aaagatcaga tcgctaccgc cgccgtggag atcgatcgtg
     1141 gtgcccaact cgcgtaccgc gagagcgctg cactcgatgt gccagccggg ccggcccggc
     1201 ccccacatcg tgtcccacga cggctcgtcg gacgccgacg ggtgccacag cacgaagtcg
     1261 agcgggtcgc gcttgtgcgg gtcgtcgacg ttgccgcccc gctcgcgggc gatctcgatc
     1321 atctcctcgc gcgacaggtg cgacacggtg ccgaagctgg ggaacttgga gatgtcgaag
     1381 tagaccgacc cacccgcctg ataggcgtag ccccggtcga ggacccggcc gatgaacccg
     1441 cggatgtcgc tgatcgccga cgaggcccgt ggcgtgctgt gcatcggcag catgtcgagt
     1501 gcggccatgt cacgctcgaa gcgtgcctct tcgcccgcgg ccaggtcgag gtaatggacg
     1561 ccgagctcac gggccttggc gaacaacggg tcgtcgacat cggtcacgtt gcgcacacac
     1621 ttgacctggt ggccgaggtc gatcaggcgc cgctggagaa cgtcgtagat cacgaacgtc
     1681 gccgcgtgcc cgaggtgggt cgcgtcgtac ggcgtgatgc cgcacgtgta catcgtgacc
     1741 aacggtccgg gttcgaacga aacgaccgcg cgccgggctg tgtcgtacaa acgcatcgtc
     1801 acgacggcga cctgtcagtc ctgtgcggtg cccgcggcag ccggctccga cggggacgtc
     1861 gtcggggcga cgacggtcgg agccgctggg ctcgatgcgg cagtcgccgc tcgcttgcgg
     1921 gggtcgtcct tgattccggt cagcttgggc gcgacccggg tcttgtagcg gatgttgagc
     1981 tgcaacaaca cggccgtgaa ggcttcgatc gcggtggcgt tgctgagctc gcccgcgtcg
     2041 agcggccggc agccagggat cttggcgacg atctcggcga ccacctcggt ggccgtcttg
     2101 tcgtcgctgc agatcaacac gtcggactcg atcggttcgc cgaggtggcc gagttcggac
     2161 gcgggcaggt gatggaacgc ggcgaccact cggcagcgcg gcacagcggc ctgcacgtgg
     2221 gcggcgacgc tcccgcgcgg cggcaccagc ggctggaact caccaccgac gcggacgagc
     2281 gcgttnnnnn nnnnnggcac ggtccgcacc acgacgccca aaagttgatg accaccacct
     2341 tgccgcgcaa cagctcttcg tactcctggg ctgtggtggc cgcgccatcc cacggtgtcg
     2401 cgatgaccac caagtcgcag ctcgccgcgc ccgggttgtc ggcgctctcg atgcgatccg
     2461 cgatctccgg ccacctggag gtcagcttgt cgcacacctc catcgctcgg tacttcgatc
     2521 gcgagccgat gacgcaggtg tagccgacag aagccagacg cgctgcgaga gcgctgcccg
     2581 cggggccggt gccccccaag attccgatac gcataggccc tcagcctgcc atacgaagcg
     2641 cggttctcct ccacctcgct gagtcatcca gcgccgcggc tcggcagtag ggtcgcaacc
     2701 atgggtctgc tctctgggaa gaacatcgtc gtcaccggcg tgctcaccga cgcgtcgctc
     2761 gcgtacggtg tcgccaagct ggcacagcag gagggagcga acatcgtgct caccggagcg
     2821 ggtcgggcgc tgagcctgac cgagcgcacg gcgcgcaagc tcgacacgcc cgtgccggtc
     2881 tacgagttcg acgtgaccgt cgccgagcac gtcgtgacgg tgcgcgaggc gctggccgag
     2941 cgatggggcc gcgtcgacgg cgtgctgcac tcgatcggct tcgccccgcc gagctgtctc
     3001 ggcgacgact tcatgggtgc gccgtgggac gacgtctcgg tcgcgatgaa catctcgacc
     3061 tactcgctca agactctggc cgacgcgttc gtgccgctga tgacggccgg tggctcattc
     3121 gtcgggctcg acttcgacaa ctccgtcgcc tggcccgcgt acaactggat gggtgtggcg
     3181 aagtcggcgc tcgagagcgt caaccgctat ctggccaagg agctcggccc caagggcatc
     3241 cgctccaacc tggttgcggc tggcccgatc cgcaccatgg cggccaagag catccccggc
     3301 ttcgccaagt tcgaagacgt ctggtccgac cgggcgccgc tcggctggga cgtctacgac
     3361 tcgtccgctg tcgccaaggc ggtcgtcgcg ctgctcagcg actggttccc ggccaccacg
     3421 ggcgagatcg tccacgtcga cggcgggttc cacgcggtcg cggtctgatg aaccgccttg
     3481 cgaccgagac gtcgccctat ctacgccagc acgcggacaa tccggtcgat tggtatccgt
     3541 ggggtgacga ggcgttcgcc gcggcccaag cccgcgacgt gccggtgctg ttgagcatcg
     3601 gctattcggc ctgccactgg tgccacgtga tggcccacga gtgcttcgaa gacgccgagg
     3661 tcgcggcggt gatgaacgag cacttcgtgt cgatcaagct cgaccgggaa gaacgacccg
     3721 acgtcgatgc ggtgtacatg gaggcgatcc aggcgatggg ccagcgtggc ggctggccgc
     3781 tgaccgtgtt cctgacgccc gaccggatgc cgttctacgg cggcacctac taccccaagc
     3841 cgctgttcct gcagctgatg gccaagatcg acgaggtctg gcaccagcag cgggccgagg
     3901 tcaccggcaa cacccaggcg ttgttccagg ccctgtcgcg ttcggccgcg acgacaccca
     3961 ccgacacctt tcccacccac gagctggtca actcggcgtt gcagggcttg gggcgcgcgt
     4021 tcgacagcgc gtggggcggc ttcggcacgc cgccgaagtt cccctcgacc atgaacctcg
     4081 atctggtgat ccgagccttc ggtcggcgac cgacccgcga cgcggcgacg gtgttcacca
     4141 ccaccctcga cgcgatggcc agcggcggca tgtacgacca catcggcggt ggcttcgccc
     4201 gttacagcgt cgaccgcgag tggctggtgc cgcacttcga gaagatgctc tacgaccagg
     4261 cgctgctggc gcgggtgtac ctgcacggtt ggcaggtcac gggcaacgag cactggcgcc
     4321 aggtggtcga ggagacgatc ggctacgtgc tgaccgacat gacccatccg ctcggcgggt
     4381 tcttgtccgc cgaagatgcc gactcactcg gtcccgacgg gcacagccac gaaggcgcgt
     4441 acagcacctg gacccccgac gaggtcgtcg cggtgctcgg ccccgagctg ggccgggcgg
     4501 ccaacgagtg gtacggcatc accgccgagg gcaatttcga atcgcgctcg attcccaacc
     4561 ggctcgctca ccggggcggg ttgatccgcc cgccagagat cgaccgggcg cgcaccttgt
//
LOCUS       DAORNF010000191         4614 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_13904_length_4614_cov_1.312788, whole genome shotgun sequence.
ACCESSION   DAORNF010000191 DAORNF010000000
VERSION     DAORNF010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4614
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13904_length_4614_cov_1.312788"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1194
                     /locus_tag="PLV68_08815"
     CDS             <1..>1194
                     /locus_tag="PLV68_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HRE01388.1"
                     /translation="AAEPDHGIPAEVLHLAAEAEDMPRHLGIHSGGMVICDRPVIEVC
                     PVEWARMADRSVLQWDKDDCAAIGLVKFDLLGLGMLSALHGAIDLVREHQGYEIDLAT
                     LQQDPLVYDMLCRADTVGVFQIESRAQMGTLPRLKPRTFQDLVIEIALIRPGPIQGGS
                     VHPYIRRRNGQEPVTYPHPLLRKSLEKTLGIPLFQEQLMQMAIDLAGFTPAEADQLRQ
                     AMGSKRSRAKMEQLRERLFAGMAERGITGDTAEELFVKMAAFANYGFPESHSQSFAYL
                     VYASAWIKLYEPAAFCAALVNAQPMGFWSPHSLVQDARRHGVHTRIPDLNASLAQATL
                     QRDADSFTGQAVRLGLSSVRGVGTSLADAIVAERDANGAFASIEDLARRVPAVTLTHL
                     ESMATA"
     assembly_gap    1195..1204
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1205..1763
                     /locus_tag="PLV68_08820"
     CDS             <1205..1763
                     /locus_tag="PLV68_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HRE01389.1"
                     /translation="ALWSVGAVAQSRPGRLSGIVTGAAAPRLAGMEPAEEAVADLWAT
                     GIAPTGHPTQFVRDELTRLGVVTSEGLWSVEPDRTVHVAGVVTHRQRPMTAQGTTFMN
                     LEDETGLINVVISKGCWARFRRVARESPALLVRGRLERSGMQARADGSTDSRADSRGV
                     INIVAEHLTPLSLHTGNVTASRDFR"
     assembly_gap    1952..2076
                     /estimated_length=125
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2077..2359)
                     /locus_tag="PLV68_08825"
     CDS             complement(<2077..2359)
                     /locus_tag="PLV68_08825"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HRE01390.1"
                     /translation="MSDERAPEPESELSVEPEPDVTGLRIRQAVRGLVIDDDRRVLLV
                     QFRLPTATVWALPGGGLDPGEDAHAGLRRELREELGLDECSIGAHLWNRL"
     gene            complement(2352..>2592)
                     /locus_tag="PLV68_08830"
     CDS             complement(2352..>2592)
                     /locus_tag="PLV68_08830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3039 domain-containing protein"
                     /protein_id="HRE01391.1"
                     /translation="EELQTGEPAVSHIVKTEPGESAAAKVLEARIYGTQLEALCGHVW
                     VPSRDPKQLPLCEKCKSVYELYKAFNDGLRDTPDE"
     assembly_gap    2593..2664
                     /estimated_length=72
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2738..3295
                     /locus_tag="PLV68_08835"
     CDS             2738..3295
                     /locus_tag="PLV68_08835"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HRE01392.1"
                     /translation="MTSDRETAATGLLHLYDRAMPQVYGYLVRRCGVTTVAEDLTADV
                     FMAAVAAIRNEVVTDITVPWLIGTARHKLIDHWRRSERQQRAFRLLGEDEAILDSHDE
                     PISAVRAHDVLEQLSTDHRAALTLRYIDDLPVADVARALDRSEHATESLLQRAKAAFR
                     HVAAMHDASQHDTSQHDTSQHGEEQ"
     gene            3295..4329
                     /locus_tag="PLV68_08840"
     CDS             3295..4329
                     /locus_tag="PLV68_08840"
                     /inference="COORDINATES: protein motif:HMM:NF037170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRE01393.1"
                     /translation="MNTGPDSSPQRDPFSVLRRPDAPIAPDPAFAARLRRDLQAALAQ
                     LTSPSLQPSIQEVRSMTVTPYLSVRNAAAAIDFYVAAFGAVEAHRLLGDDGRVGHAEI
                     TIGGSTVMLADEYPEIDSVGPETRGGPTCTFTITVTDADHAYQRALDAGAIGLREPAD
                     QFHGNRSAIVADLFGQRWTLSAPIEQLTTEQYAARAAEDHGHGSFHLETPDPESGSGA
                     PGAESFAHQVKALAPGDLYYFTLPVADLPKAQTFFGAVLGWRFAAPDQGHVENISAPP
                     GGVNDRGDDTGARLWFVVDDIHTAIDRVLAAGGTADEPILYDSGWSADCTDDQGTIFS
                     LSVPAAKYTA"
BASE COUNT          722 a         1562 c         1453 g          670 t
ORIGIN      
        1 gcggccgaac ccgaccacgg catcccagcc gaggtgctgc acctggcggc cgaagccgag
       61 gacatgccgc gccatctggg catccactcc ggtggcatgg tgatctgcga ccggccggtg
      121 atcgaggtgt gcccggtcga atgggcccgc atggccgacc gcagcgtgtt gcagtgggac
      181 aaggacgatt gcgccgccat tggcctggtc aagttcgacc tgctcggctt gggcatgctc
      241 agcgcgctgc acggcgcgat cgacctggtg cgcgagcacc agggctacga gatcgatctg
      301 gcgacactgc aacaggaccc gctggtctac gacatgttgt gccgggccga caccgtgggg
      361 gtgttccaga tcgagagccg ggcccagatg ggcaccctgc cgcggctcaa gccgcgcacg
      421 ttccaggacc tggtgatcga gatcgccctg atccgccccg gccccatcca aggtgggtcg
      481 gtacacccgt acatccgccg ccgcaacggg caggagccgg tcacctaccc gcacccgttg
      541 ttgaggaaat cactggaaaa gacactgggc ataccgctgt tccaagagca gttgatgcag
      601 atggcgatcg acctggccgg tttcacgccg gccgaggccg accagcttcg tcaggcgatg
      661 gggtcgaagc ggtcacgggc caagatggag cagctccgcg agcggctgtt cgcgggcatg
      721 gccgaacgag gcatcaccgg cgacaccgcc gaggagctgt tcgtcaagat ggcggccttt
      781 gccaactacg gctttcccga gtcgcactcg cagtcgttcg cctacctggt ctatgcctcg
      841 gcatggatca agctgtacga accggctgcg ttctgcgccg cgctggtcaa cgcccagccg
      901 atggggttct ggtcgccgca ctcgctggtg caagacgccc gccgccacgg ggtgcatacc
      961 cgcatccccg atctcaacgc ctcgctggct caggcgacgc tgcagcgcga cgccgattcg
     1021 ttcaccggtc aggcggtgcg attggggctg tcgtcggtgc gcggtgtcgg cacaagcctg
     1081 gccgatgcga tcgtcgccga acgcgacgcc aacggggcct ttgcgtcgat cgaagatctc
     1141 gcccggcggg tgcccgccgt caccttgacc catctcgaat cgatggccac cgccnnnnnn
     1201 nnnnagcact gtggtcggta ggcgcggtgg cccagtcacg acccgggcgt ctgagcggca
     1261 tcgtcaccgg cgccgcggcg ccgcggctgg cgggcatgga gccggccgaa gaggccgtcg
     1321 ccgacctgtg ggccaccggc atcgccccca ccggccaccc cacgcagttc gtgcgtgacg
     1381 agctgacccg cctcggggtc gtcacctccg agggattgtg gtcggtcgaa cccgatcgca
     1441 cggtccacgt cgccggggtg gtcacccatc gccagcggcc gatgaccgcg cagggcacca
     1501 cgttcatgaa cctcgaagac gagaccggcc tgatcaacgt ggtgatctcc aagggctgct
     1561 gggcccgctt tcgccgcgtc gctcgcgagt cgccggccct gctggtacgt ggccgactgg
     1621 aacggtcagg catgcaagcc cgtgccgacg gcagcaccga cagccgtgcc gacagtcgcg
     1681 gcgtgatcaa catcgtcgcc gaacacctca ccccgctgtc gctccacacc ggcaacgtca
     1741 ccgccagccg cgacttccgc tgacggcatg cctgtttcgt gacaggccgg cgcgcgtcgc
     1801 gtcagacgcc ggtggtcagt ggggttgggg gtgggccttc gtggaagtag cgggcgagga
     1861 agtgggacag gcggcgggga acgaacagct cggtcgacga ggcgatctcg ggtggtgtcc
     1921 accagcgaat gtcgtagaga tgctcggcgt tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngcaa gcggttccag aggtgggccc
     2101 cgatcgagca ctcgtccaga ccgagctctt cgcgcagctc gcggcgcagc ccggcgtggg
     2161 cgtcctcacc ggggtcgagc ccgccgcccg gcagggccca cacggtcgcg gtgggaaggc
     2221 ggaactgcac cagcagcacg cgccggtcgt cgtcgatcac caacccacga acggcctgac
     2281 gaatgcgcaa cccggtcaca tcgggctcgg gctccacact gagttccgac tcgggctccg
     2341 gtgcccgttc gtcactcatc gggcgtgtcg cgcaggccgt cgttgaaggc cttgtacaac
     2401 tcgtagacgc tcttgcactt ctcgcacagc ggcagttgct tgggatcgcg cgacggcacc
     2461 cacacgtggc cgcacagagc ttccagctgg gtgccgtaga tacgggcctc gagcaccttg
     2521 gccgcggccg attcgcccgg ctcggtcttg acgatgtgag agacggctgg ctcgccggtc
     2581 tggagctctt cgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnngccatg agtcggagtg tgcaagattt cttcgggttc
     2701 gcgacggaac gagggcgcgc cagacgatgg aggtggcatg acgagcgatc gggagactgc
     2761 tgccacaggg ctgctgcacc tctacgaccg cgccatgccc caggtctacg gctatctcgt
     2821 ccgccgttgc ggcgtcacca ccgtggccga ggacctgacc gccgacgtgt tcatggctgc
     2881 cgtcgccgcg atccgcaacg aggtcgtcac cgacatcacg gtcccctggc tgatcggtac
     2941 tgcgcgccac aagctgatcg accactggcg acgctcagaa cgccagcaac gggcgttccg
     3001 gctgctcggc gaggacgaag cgatcctcga ctcccacgac gagccgatca gcgcggtgcg
     3061 tgctcacgac gtgctcgaac aactcagcac cgatcatcgc gccgcgctca ccttgcgcta
     3121 catcgacgac ctgccggttg ccgacgtcgc ccgcgccctc gatcgcagcg agcacgccac
     3181 cgagtcactg ctgcaacggg ccaaggccgc cttccgccac gtagcagcga tgcacgacgc
     3241 cagccagcac gacaccagcc agcacgacac cagccagcac ggagaggagc agtgatgaac
     3301 accggtcccg actcgtcgcc acagcgcgat ccgttcagcg tgctgcgccg gcccgacgcc
     3361 ccgatcgccc ccgaccccgc ctttgcggca cgcctacgcc gcgatctgca ggccgcgctc
     3421 gcccaactca catcgccatc cctacagcca tccatccagg aggttcgttc catgaccgtc
     3481 accccgtacc tcagcgttcg caatgccgcc gcggcgatcg acttctacgt cgctgccttc
     3541 ggcgcggtcg aagcccaccg cctgctcggc gacgacggcc gcgtcggcca cgccgagatc
     3601 accatcggcg gctcgacggt gatgctcgcc gacgagtacc ccgagatcga ttcggtcggc
     3661 cccgagaccc gcggcgggcc gacctgcacg ttcacgatca ccgtcaccga cgccgaccac
     3721 gcataccagc gtgcactcga cgctggcgcc atcgggctgc gcgaaccggc cgaccagttc
     3781 cacggcaacc gatcggcgat cgtggctgac ctcttcgggc agcgctggac tctcagcgcg
     3841 ccgatcgagc agctgaccac cgagcagtac gcggcgcgcg ccgcggaaga ccacggccac
     3901 gggtcgttcc atctggaaac acctgacccc gagtccggtt ccggagcgcc cggtgcggag
     3961 agcttcgccc atcaggtcaa ggcgctggct cccggcgacc tgtactactt caccctgccc
     4021 gtggccgacc tgcccaaggc tcagaccttc ttcggcgcgg tgctcggctg gcgcttcgcc
     4081 gcccccgatc agggtcacgt cgagaacatc tccgctccac ccggcggcgt caacgaccgc
     4141 ggcgacgaca ccggtgcccg gctgtggttc gtggtcgacg acatccacac cgcgatcgac
     4201 cgggtcctcg ccgctggtgg caccgccgac gagccgatcc tctacgactc aggctggtcc
     4261 gccgactgca ccgacgacca aggcacgatc ttcagcctca gcgtccccgc cgccaagtac
     4321 accgcctgac ccccggggag atccagccga ccggggactg tgttcgattg gcccctgtca
     4381 cgggggtgga tcgaacacac aaggcgtggc gaccggggac tgtgttcgat tggcccctgt
     4441 cacgggggtg gatcgaacac aaggcgtggc tgcgggttac ggggagcggg tgaggaggtg
     4501 aatggagtag gccggacctt gggcgcggag ggcggcgagt tcggtgacca cgtcggcggg
     4561 cacgccggag cgcaccaaca tctcgcggct cgcagggcgg tcgaagtcgc tgag
//
LOCUS       DAORNF010000192         4607 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_13933_length_4607_cov_1.672232, whole genome shotgun sequence.
ACCESSION   DAORNF010000192 DAORNF010000000
VERSION     DAORNF010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4607
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13933_length_4607_cov_1.672232"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..814
                     /locus_tag="PLV68_08845"
     CDS             <1..814
                     /locus_tag="PLV68_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit C"
                     /protein_id="HRE01394.1"
                     /translation="PCRRAWARRVRRHRLRRASDHNSRSRALTELQDLLGLPEAPLRI
                     ECYDMAHMQGSDYVGSMVVLEDGIPNKREYRRFKVKTVAGNDDYAAMEEVLTRRLRAY
                     LDERDQPVNERGDRPGKFAYPPQLLVVDGGKGQLAVAERVVRELGLTDEIPLASLAKR
                     FEEVYLPGQNLPVNVPRGSEALFMLQRIRDEAHRFANSFHRELRGKRMTKSVLDDVPG
                     LGEVRKKRLLKELGGITAVRQAPLATLTALTWLPDAVATAVYTALHSSSTEA"
     gene            complement(778..1776)
                     /locus_tag="PLV68_08850"
     CDS             complement(778..1776)
                     /locus_tag="PLV68_08850"
                     /inference="COORDINATES: protein motif:HMM:TIGR03718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HRE01395.1"
                     /translation="MSPIFASAPASAAKKQFVNLDVPLWCWLLLGGLIVAMLAIDLFR
                     HRDDHEPSPKEALTESLIWIGCGLAFSGFVALQFGGPAFGEYISGYLIEKSLSVDNVF
                     VWSMLFTSMAIPLKYQHRVLFWGIFGALALRAIFVLAGTALITKFWWVLLIFGVFLIY
                     TGIKIFRHRNEDEVHEGTAGLNLLQKFMPVSKEFDGHKFVTRINGKKAATPLLAALVV
                     VEATDIIFAVDSVPAILAVSHETFIVLSSNAFAILGLRAMYFLLANARDSFHYLSHAL
                     GAILVFVGIKMTIAHWFHMNTYVSLAVIVSMLTAGIVASILHNKRQASVEEECSAV"
     gene            2010..>2406
                     /locus_tag="PLV68_08855"
     CDS             2010..>2406
                     /locus_tag="PLV68_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRE01396.1"
                     /translation="MDTEFAAASSAADGLWERHADWWIDGFTEGADPEYVEQILPLAA
                     RELAGAARVLDVGCGDGQVSRLAAALGAESVVGIDPTWNCISVAHGRGGGAAFAQAGA
                     AALPFADASFDAVVACLVFEHIRDVDDAIA"
     assembly_gap    2407..2421
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2422..>2729
                     /locus_tag="PLV68_08860"
     CDS             <2422..>2729
                     /locus_tag="PLV68_08860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRE01397.1"
                     /translation="VLVPGGRFCFFLNHPLLQTPNSGWIDDQFLDPPEQYWRIGPYLV
                     EDESLQEVEKDVFIPFIHRPLGRYVNTLAANGLLVERMEEPAPPAGFLALAEEYEEAA
                     S"
     assembly_gap    2730..2848
                     /estimated_length=119
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2849..>3205
                     /locus_tag="PLV68_08865"
     CDS             <2849..>3205
                     /locus_tag="PLV68_08865"
                     /inference="COORDINATES: protein motif:HMM:NF015620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HRE01398.1"
                     /translation="GWFVVDNLPVTLIDKIVELSSTSSGGGIPRLALVVGPTADQAAV
                     IAEIHRLRAGGQRVRVLFLDASTGELVKRYGSTRRKHPLDDGAGITMAIERERALLEP
                     VKAEADMVLDTTALTVH"
     assembly_gap    3206..3304
                     /estimated_length=99
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3305..3620
                     /locus_tag="PLV68_08870"
     CDS             <3305..3620
                     /locus_tag="PLV68_08870"
                     /inference="COORDINATES: protein motif:HMM:NF015620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HRE01399.1"
                     /translation="DVVMDVRFLPNPHWVEELRPLTGLDEPLRRYVLDQPVTGEFLRR
                     FEDLLELLVPAYINEGKSYLSIAIGCTGGQHRSVAIAEELAQWLRRIGHTPRVAHRDL
                     PR"
     gene            3773..>4607
                     /locus_tag="PLV68_08875"
     CDS             3773..>4607
                     /locus_tag="PLV68_08875"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004365 - glyceraldehyde-3-phosphate
                     dehydrogenase (NAD+) (phosphorylating) activity [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HRE01400.1"
                     /translation="MTVRVGINGFGRIGRNFFRAAKQRGADIDFVAVNDLGSLETMAH
                     LLKYDSVLGVLPDDIKAVKGGIKVGNDTLRVLQERNPADLPWGELGVDVVIESTGIFT
                     DRDKAALHLEGGAPYVIVSAPATGADATFVYGVNHTDFDPKKDKVISNASCTTNCFVP
                     MMKVLDDAFGVENGLMTTIHAYTGDQSLVDGPHKDLRRARAAAINIVPTSTGAARATA
                     LVMASMKGKLDGTSLRVPVPTGSITDFTGNLKRAASVEEINAAFKAAAAKGALKGVLK
                     YT"
BASE COUNT          767 a         1425 c         1477 g          705 t
ORIGIN      
        1 accatgtcgg cgcgcatggg cgaggcgggt ccgccgccac cggctgcgcc gcgcctccga
       61 ccacaacagc cgcagccggg cgctgaccga gttgcaggac ctgctcggcc tgcccgaagc
      121 gccattgcgg atcgagtgct acgacatggc ccacatgcag ggcagcgatt acgtcggctc
      181 gatggtggtg ctcgaagacg gcataccgaa caagcgtgag taccgtcgtt tcaaggtcaa
      241 gaccgtggcc ggcaacgacg actacgcggc gatggaagag gtgctcaccc gccgcctgcg
      301 ggcctacctg gacgagcgcg accagccggt caacgagcgc ggtgaccggc cgggcaagtt
      361 cgcctacccg ccgcagttgt tggtggtcga cggcggcaaa ggccagctcg cggtggccga
      421 acgggtcgtg cgcgagctcg gcctcaccga cgagatcccg ctcgcgtcgt tggcgaaacg
      481 cttcgaagag gtctacctgc ccggccagaa cctgccggtc aacgtgcccc gcggcagcga
      541 ggcgttgttc atgctgcagc ggatccgtga cgaggcccac cgcttcgcca acagcttcca
      601 ccgcgagctg cgcggcaagc ggatgaccaa gagtgtgctc gatgacgtcc ccggtctcgg
      661 cgaggtccgc aagaagcggc tgctcaagga gctcggtggc atcaccgccg tgcgtcaggc
      721 acccctcgcc acgctcacgg cgttgacgtg gctgcctgac gctgtggcga cagcggtcta
      781 caccgcgctg cactcctctt cgaccgaggc ctgacgcttg ttgtggagga tgctggcgac
      841 gatgccagcg gtcagcatcg acacgatcac ggccagcgac acgtaggtgt tcatatggaa
      901 ccagtgggcg atcgtcatct tgatgccgac gaacaccagg atcgcgccca gggcgtggct
      961 cagatagtgg aagctgtcgc gggcgttggc gagcaggaag tacatcgccc gcaggccgag
     1021 gatggcgaac gcgttggacg acagcacgat gaacgtctcg tgtgacacgg cgaggatcgc
     1081 cggcaccgag tcgacggcga agatgatgtc ggtcgcttcg accaccacca gcgcggcgag
     1141 cagcggcgtc gccgccttct tgccgttgat ccgggtgacg aacttgtgcc cgtcgaactc
     1201 cttgctgacc ggcatgaact tctgcagcag gttgagcccc gccgtgcctt cgtggacctc
     1261 gtcctcgttg cggtggcgga agatcttgat gccggtgtag atcaggaaca cgccgaagat
     1321 cagcagtacc caccagaact tggtgatcag cgcggtgccg gcgagcacga agatggcccg
     1381 cagcgcgagg gcaccgaaga tgccccagaa cagcacgcgg tgttgatact tcagcgggat
     1441 cgccatcgac gtgaacaaca tcgaccacac gaacacgttg tcgacgctga gcgacttctc
     1501 gatgaggtag ccgctgatgt actcgccgaa cgccgggccg ccgaactgga gtgcgacgaa
     1561 gccggagaag gccagtccgc agccgatcca gatcagcgat tcggtcaacg cctccttcgg
     1621 cgacggttcg tggtcgtcgc ggtgacggaa caggtcgatg gccagcatcg cgacgatgag
     1681 gccgcccagc aagagccaac accacagcgg tacgtcgagg ttgacgaact gcttcttggc
     1741 ggcagaggcg ggggccgagg caaagatcgg agacatgggt tgggggtgca tacttcctga
     1801 acgagggccg cgtgatggcc cggcgacagg aggtcttccc cgcctcggtg ccgacgagac
     1861 agtcttgcga gagatcggtg gcgctgagga gacagaccgg acgccggcga gcggcgacgt
     1921 gttgacgacc tgtcgttggg tggggtactc ccctccgggg gagaaactct accgtacgct
     1981 gcctggcgag aaagggagtg cggatgcaca tggatacgga atttgcggct gccagttcgg
     2041 cggcggacgg cttgtgggag cgccatgccg actggtggat cgacgggttc accgaaggtg
     2101 ctgatcccga gtacgtcgag cagatcctgc cgctcgcggc gcgagagctc gcgggcgcgg
     2161 cgcgggtgct cgacgtcggc tgcggtgacg gccaggtcag ccggctggca gccgcgctcg
     2221 gggccgagtc ggtcgtgggc atcgacccca cctggaactg catcagcgtc gctcatggcc
     2281 gcggcggggg agcagcgttc gcgcaggccg gtgcggccgc gctgcccttc gccgatgcca
     2341 gcttcgatgc agtcgtggcg tgcttggtgt tcgagcacat ccgcgacgtc gacgatgcga
     2401 ttgccgnnnn nnnnnnnnnn ngtgctcgtg cccggcggcc ggttctgctt cttcctcaac
     2461 cacccgctgc tccagacgcc caacagcggc tggatcgacg accagttcct cgacccgccc
     2521 gagcagtact ggcgcatcgg gccgtacctg gtcgaggacg agtcgctgca ggaggtggaa
     2581 aaggacgtgt tcatcccctt catccaccgc ccgctcggtc gttacgtcaa caccttggcc
     2641 gccaacgggt tgttggtcga acggatggaa gagcccgcgc cgcccgccgg gtttctcgcg
     2701 ttggccgagg agtacgaaga ggcggcgtcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnngg ctggttcgtc gtcgacaacc tgccggtcac
     2881 gctgatcgac aagatcgtcg agctcagttc gaccagctct ggtggcggca tccctcgttt
     2941 ggcactggtg gtcgggccca cggccgacca ggcggcagtg atcgccgaga tccaccgtct
     3001 gcgcgccggc ggccagcggg tgcgggtgct gttcctcgac gccagcacgg gcgagttggt
     3061 caagcgctac ggcagcacgc ggcgcaagca ccccctcgac gatggtgccg gcatcacgat
     3121 ggcgatcgaa cgggagcgag ccctgctgga gccggtcaag gccgaagccg acatggtgct
     3181 cgacaccacg gccctgaccg tccatnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnncgacgt cgtgatggac gtgcgcttct tgcccaaccc gcattgggtc gaagagctgc
     3361 ggccgttgac ggggctggac gaacccctac gccgttacgt gctcgaccag ccggtgacgg
     3421 gtgagttcct gcgccgtttc gaagacttgc tcgaactgct ggtgcccgcg tacatcaacg
     3481 agggcaagag ctacctgtcg atcgccatcg gctgcaccgg cgggcagcac cgttcggtgg
     3541 ccatcgccga agagctcgcg caatggttgc gccgcatcgg ccacaccccg cgagtcgcgc
     3601 accgcgacct gccgcgctga accgggacgt tcggggctga accgggacgt tcgtcagttc
     3661 tgctcgtccg tgcgggagcg cttcttcggc tcgtcgtggc ccggcgacta cagtcgccaa
     3721 cgactaccca ggtcggcgcc ctggcgcttc actctccgaa aggctgtcaa cgatgacggt
     3781 tcgtgttggt atcaacggat ttggccggat cggccgcaac ttcttccgcg ctgccaagca
     3841 gcgtggcgcc gatatcgact tcgtcgccgt caacgacctg ggttcgttgg agacgatggc
     3901 tcacctgctc aagtacgact cggtgctggg cgtgttgccc gacgacatca aggcggtcaa
     3961 gggcggtatc aaggtcggca acgacacgct gcgggtgctg caagagcgca accccgccga
     4021 tctgccgtgg ggcgagttgg gtgtcgacgt ggtcatcgag tcgaccggca tcttcaccga
     4081 ccgcgacaag gccgcgctgc acctcgaagg cggcgcaccg tacgtgatcg tcagcgcccc
     4141 cgcgaccggg gccgacgcca cgttcgtcta cggcgtcaac cacaccgact tcgacccgaa
     4201 gaaggacaag gtcatctcca acgcgagctg caccaccaac tgcttcgtcc cgatgatgaa
     4261 ggtgctcgac gacgcgttcg gcgtcgagaa cggcctgatg accaccatcc acgcctacac
     4321 cggtgaccag agcctggtcg acggtcccca caaggacctc cgccgcgccc gtgccgcagc
     4381 gatcaacatc gtgccgacca gcaccggtgc cgctcgcgcc accgcgctgg tcatggcgtc
     4441 gatgaagggc aagctcgacg gcacctcgct gcgcgtcccg gtgccgaccg gttcgatcac
     4501 cgacttcacc ggcaacctca agcgcgccgc cagcgtcgaa gagatcaatg ccgcgttcaa
     4561 ggccgccgcc gccaagggtg cgctgaaggg tgtgctcaag tacaccg
//
LOCUS       DAORNF010000193         4599 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_13959_length_4599_cov_1.704886, whole genome shotgun sequence.
ACCESSION   DAORNF010000193 DAORNF010000000
VERSION     DAORNF010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4599
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13959_length_4599_cov_1.704886"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>396)
                     /locus_tag="PLV68_08880"
     CDS             complement(<1..>396)
                     /locus_tag="PLV68_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HRE01401.1"
                     /translation="LGFGEGVAGHITVRDPEFPDHFWVNPFGKSFRHMTVSDLILVNH
                     SGEVVYGDQPVNRAAFVIHAALHQARPDVVAAAHSHSVYGKAFSSLGIPLAPLTQDSC
                     IFYQDHVVIAEHGGAVAFEVDAGNDLAAAF"
     assembly_gap    397..417
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<418..600)
                     /locus_tag="PLV68_08885"
     CDS             complement(<418..600)
                     /locus_tag="PLV68_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01402.1"
                     /translation="MAILENESHGAAPSHDAPKMIDGVELTPKVLSMAFAPRQFDTVA
                     DERRERKQKLAGALRIF"
     assembly_gap    658..672
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <673..1043
                     /locus_tag="PLV68_08890"
     CDS             <673..1043
                     /locus_tag="PLV68_08890"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HRE01403.1"
                     /translation="TPADAAPPAANYALAVVAERPQRLLHTSGIVPVAPDGSVPTDVA
                     DQAEVVWASLLAILAEAGMGVADVVSITTYVVAGEPLGGVMAARDRALGGHRAASTLV
                     TVPALAQAAWKMEIALVAAS"
     gene            1097..>1293
                     /locus_tag="PLV68_08895"
     CDS             1097..>1293
                     /locus_tag="PLV68_08895"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRE01404.1"
                     /translation="MTDQGAPAHGLVVERSGSGTPIVLLHGIGMSKSTWHPLRDAFAA
                     AGDRFETFAIDLPGHGDSVVT"
     assembly_gap    1294..1319
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1320..>1540
                     /locus_tag="PLV68_08900"
     CDS             <1320..>1540
                     /locus_tag="PLV68_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRE01405.1"
                     /translation="VLAHLDGLLAEIGQPVVLVGHSLGGYLSLAYVASRPEAAVSGVV
                     VLNTGPGFRDPVKREGWNERSRRNWHRFGV"
     assembly_gap    1541..1584
                     /estimated_length=44
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1585..1807
                     /locus_tag="PLV68_08905"
     CDS             <1585..1807
                     /locus_tag="PLV68_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01406.1"
                     /translation="VMDRLDQITTPVLFLAGSADRADYANSGEYLSRKMPACRFMLID
                     QGEHSMHESQPDVVAAAIREFAGGLASAT"
     assembly_gap    1861..1884
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1885..2826)
                     /gene="dapD"
                     /locus_tag="PLV68_08910"
     CDS             complement(<1885..2826)
                     /gene="dapD"
                     /locus_tag="PLV68_08910"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HRE01407.1"
                     /translation="MTLAWGRGVATIAADGSTLDSWFPNLGLGAAPDHITTDLTALAG
                     SDPVRNVSTTLVSLEIDTDTAPVSASDVYLRLHLLSARLMQPRTINLDGQFGLLANVA
                     WTNLGPVAIGEVDATRIRARSAGQPLQVFGVDKFPRMTDYVVPEGVRIADADRVRLGA
                     HLAAGTTVMHEGFCNFNAGTLGTSMVEGRITAGVVVGDGSDIGGGSSIMGTLSGGGTH
                     QVTIGERCLIGAEAGIGISLGDGCVVEAGLYVTAGTRVTVLPEGTVVKARQLSGAGGL
                     LYRRNSETGRVEALPRSVDWGGLNSVLSHLRGEFVIIL"
     gene            complement(2839..>3551)
                     /locus_tag="PLV68_08915"
     CDS             complement(2839..>3551)
                     /locus_tag="PLV68_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HRE01408.1"
                     /translation="RHNPEFTMMESYEAFADVSDVIALTERLITNAARDALGTTVVEL
                     GGKPVDLAQPWRQVRMIDLVHEATGVAVHPSQPVDDLRALAAEHGVSCAPRWGSGKII
                     EELFEATSEHLLIEPTFVTGHPVEISPLARVDRNDPFLTERFELFVNGREHANGYSEL
                     NDPVEQRLRFEDEQRAKEAGDDEAGTLDEDYIRALEYGLPPTGGLGIGMDRLAMTLAN
                     VASIKEVILFPTLRPEVF"
     assembly_gap    3552..3646
                     /estimated_length=95
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3647..4390)
                     /locus_tag="PLV68_08920"
     CDS             complement(<3647..4390)
                     /locus_tag="PLV68_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HRE01409.1"
                     /translation="MNDVPDNLQPTVETDDASEVASGSGLAAERAKRLAKLDAMREAG
                     IEPYPYRFDRTHTLAEVRAAHGEIEAGAETDAEVRLAGRMMLRRDTGKLVFAQLRDRT
                     DQLQLFISKAVVGDDTFATVGELDLGDWIGVEGTVMKTRTGELSIKVRSIVLLAKAVR
                     PMPDKWKGLTDTDTRFRQRYTDLIVNDEARRAFEIRHAIIASFRSTFATLGFTEVETP
                     ILHVEVGGAHARPFVTHHNTLDLDMYLRIA"
BASE COUNT          647 a         1416 c         1580 g          731 t
ORIGIN      
        1 gaacgcggcc gccaggtcgt tgccggcatc gacttcgaac gcgactgcgc cgccgtgttc
       61 ggcgatgacg acgtggtctt ggtagaagat gcacgagtcc tgtgtgagcg gtgcgagcgg
      121 gatgcccaac gacgagaacg ccttgccgta caccgagtgg ctgtgggccg cggcgacgac
      181 gtcgggccgg gcctggtgca gggcagcgtg gatcacgaac gcggcccgat tgaccggctg
      241 gtcgccgtag accacctcgc cgctgtggtt gaccaggatc aggtcgctga cggtcatgtg
      301 gcggaacgac ttgccgaacg ggttgaccca gaagtgatcg ggaaactcgg ggtcgcgcac
      361 ggtgatgtgt ccggcgacgc cctcgccaaa gccgagnnnn nnnnnnnnnn nnnnnnngaa
      421 gatgcgcagg gcaccggcca acttctgctt gcgctcgcgg cgctcgtcgg cgacggtgtc
      481 gaactggcgt ggcgcgaagg ccatcgacag caccttcggc gtgagctcga cgccgtcgat
      541 catcttcggg gcgtcgtggg atggcgcggc gccgtgggac tcgttctcca ggatggccat
      601 cggcccaggt tacgcccatc gccgccgtgc caagatgccg gacgatgaac cgcgagannn
      661 nnnnnnnnnn nntcaccccc gccgacgcag ccccaccggc tgccaactac gcattggcgg
      721 tggtcgccga gcggccgcag cgactgctgc acacgtccgg catcgtgccc gtcgcgcccg
      781 acggcagcgt gcccaccgac gtcgccgatc aggccgaggt ggtgtgggcc agcctgctcg
      841 ccatcctggc cgaagcgggc atgggtgtgg ccgatgtggt gtcgatcacg acgtatgtcg
      901 tcgccggtga accgctcggc ggcgtgatgg ccgcgcgcga tcgagccctc ggtggccacc
      961 gcgcggcgag caccctggtg accgtgccgg cattggccca ggcggcgtgg aagatggaga
     1021 tcgcgctcgt cgccgcgtcg tgatcgcggc gttgtcgttg ccaaccgggc accaccggca
     1081 tctaccatcg tgacccatga ctgatcaggg ggcaccagcg cacggactcg tcgtcgaacg
     1141 gtccgggtcg ggcaccccga tcgtgctgct gcacgggatc ggcatgtcga agtcgacctg
     1201 gcacccgttg cgcgacgcgt tcgcggcagc gggcgatcgg ttcgagacgt tcgcgatcga
     1261 tctgcccggc cacggtgact cggtggtcac ggannnnnnn nnnnnnnnnn nnnnnnnnng
     1321 tgctcgcgca tctcgacggg ctgctcgcgg agatcggcca gcccgtcgtg ctggtcggcc
     1381 actccctcgg cggttacctg tcgttggcct acgtcgcctc gcggcccgaa gctgccgtca
     1441 gcggagtcgt ggtactcaac accggtccgg ggttccgcga tccggtcaag cgcgagggct
     1501 ggaacgaacg gtcgcggcgc aactggcacc gcttcggagt nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnggtgat ggaccgcctc gaccagatca ccacgccggt
     1621 gctcttcctg gccggttcgg ccgaccgggc cgactacgcc aacagcggtg agtatctgag
     1681 ccgcaagatg cctgcgtgtc ggttcatgct gatcgaccag ggtgagcact cgatgcacga
     1741 atcgcagccg gacgtggtcg cggccgccat ccgcgagttc gccggaggtc tggcgagcgc
     1801 aacgtgaggc caagcatcgt gagctgacgt cgcggcgctg gcctccgacg agaacgatgg
     1861 nnnnnnnnnn nnnnnnnnnn nnnngagtat gatgacaaac tcaccccgca gatgagagag
     1921 gacgctgttg aggccgcccc agtcgacgct gcgcgggagc gcttcgaccc ggccggtctc
     1981 gctgttgcgg cggtagagca gtccgcccgc gccactgagt tggcgggcct tgacgacggt
     2041 gccttcgggc agcacggtga cccgcgtgcc ggccgtgacg tacagcccgg cctcgaccac
     2101 gcacccgtcg ccgaggctga tgccgatgcc cgcctcggcg ccgatcaggc accgctcgcc
     2161 gatcgtgacc tggtgggtac cgccgccgga gagcgtgccc atgatcgacg acccgccgcc
     2221 gatgtcgctg ccgtcgccga cgacgacgcc cgcggtgatc cgtccctcga ccatcgacgt
     2281 gcccaaggtg cccgcgttga agttgcagaa gccctcgtgc atcacggtgg tgcccgccgc
     2341 caggtgggcg ccgaggcgca cccggtcggc gtcggcgatg cggacgccct cgggaacgac
     2401 gtagtcggtc atgcgcggga acttgtcgac gccgaacacc tgcaacggct ggcccgcaga
     2461 gcgagcgcgg atgcgggtgg cgtcgacctc accgatcgct accggtccga ggttggtcca
     2521 ggcgacgttg gcgagcaacc cgaactggcc gtcgaggttg atcgtgcgcg gttgcatcag
     2581 ccgggccgac agcaggtgca ggcgcaggta cacgtcgctg gcgctgaccg gcgcggtgtc
     2641 ggtgtcgatc tccagtgaca ccagcgtggt gctgacgttg cgcaccgggt cgctgccggc
     2701 caatgcggtc aggtcggtcg tgatgtggtc gggtgcggcc ccgagcccga ggttcgggaa
     2761 ccagctatcg agggtggaac cgtccgctgc aatcgtcgcg accccgcgac cccaagccaa
     2821 agtcatgtcg ctagtcaatc agaagacttc ggggcgcagg gtggggaaga ggatgacctc
     2881 tttgatcgag gcgacgttgg cgagggtcat cgccaagcgg tccatgccga tgccgagtcc
     2941 accggtgggg ggcagaccgt actcgagggc gcggatgtag tcctcgtcga gcgtgcccgc
     3001 ttcgtcgtca ccggcctcct tggctcgttg ctcgtcttcg aaccgcagcc gctgttcgac
     3061 cgggtcgttc agctcggaat agccgttggc gtgctcgcgc ccgttgacga acagctcgaa
     3121 ccgctcggtg aggaacgggt cgttgcggtc gacgcgggcg agggggctga tctcgaccgg
     3181 gtggccggtg acgaacgtcg gctcgatcaa gaggtgctcg gacgtcgctt cgaacaactc
     3241 ctcgatgatc ttgccgctgc cccagcgtgg cgcgcacgac acgccgtgct cggcggcgag
     3301 cgcacgcagg tcgtcgacag gctgcgacgg gtgcacggcg acgccggtcg cctcgtggac
     3361 cagatcgatc atccgtacct ggcgccacgg ctgggcgagg tcgaccggct tgccgcccag
     3421 ttcgaccacg gtggtgccga gcgcgtcgcg tgccgcgttg gtgatcagcc gctcggtcag
     3481 cgcgatcacg tcgctgacgt cggcgaaggc ttcgtacgac tccatcatcg tgaactcggg
     3541 gttgtggcgg gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncgcg atccgcaggt
     3661 acatgtcgag atcgagcgtg ttgtgatggg tgacgaaggg ccgcgcgtgg gcaccgccga
     3721 cctcgacgtg caggatcggc gtctcgacct cggtgaagcc gagcgtggcg aaggtggagc
     3781 ggaagctggc gatgatcgcg tgacgaatct cgaacgcgcg gcgagcctcg tcgttgacga
     3841 tcaggtcggt gtagcgctgg cggaaccggg tgtcggtatc ggtgaggccc ttccacttgt
     3901 cgggcatcgg tcgcaccgcc ttggccagca gcacgatcga gcggaccttg atgctcagct
     3961 cgccggtgcg ggtcttcatg accgtgccct cgacaccgat ccagtcgccg agatcgagtt
     4021 caccgacggt ggcgaacgtg tcgtcgccga ccacggcctt ggagatgaac agctggagct
     4081 ggtcggtgcg gtcgcgtagt tgggcgaaca ccagcttgcc ggtgtcgcgg cgcagcatca
     4141 tccgtccggc aaggcgcacc tcggcgtcgg tctcggcgcc cgcttcgatc tcgccgtgag
     4201 ctgcgcggac ctcggccaag gtgtgggtgc ggtcgaaccg atacgggtag ggctcgatgc
     4261 ccgcctcgcg catcgcgtcc aacttggcca gccgcttggc gcgctcggca gcgagtccgc
     4321 tgccgcttgc gacctcggac gcgtcgtcgg tttcgacggt gggctggagg ttgtcgggca
     4381 cgtcgttcac gtggctgcac ggtaaccgct ccggcgctgc agccaggctt ccgtttgcgg
     4441 gcgcaggcgt ctgcctgtct ggaccgcgga ctcaaccgcg ctgggagtcc ctacagtgac
     4501 agcccgtgac ggtgagatcg cttcgctctg tgcaggtggg gtcgcttcgc gctgtgcagg
     4561 tgagatcgcg tcgcgctgtg caggtgaggt cgcgtcgcg
//
LOCUS       DAORNF010000194         4557 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_14123_length_4557_cov_1.818969, whole genome shotgun sequence.
ACCESSION   DAORNF010000194 DAORNF010000000
VERSION     DAORNF010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4557
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14123_length_4557_cov_1.818969"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>331)
                     /locus_tag="PLV68_08925"
     CDS             complement(<1..>331)
                     /locus_tag="PLV68_08925"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRE01410.1"
                     /translation="YVIRNRANDPQNRIGTLLVNPGGPGFGGSFMAESADFIYGTALT
                     DHFDIVGWDPRGTGLSEPSIDCIDDYDPYFALDSSPDDDAERQAILDAAADFGAACLE
                     RSGAILP"
     assembly_gap    332..346
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<347..656)
                     /locus_tag="PLV68_08930"
     CDS             complement(<347..656)
                     /locus_tag="PLV68_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01411.1"
                     /translation="MPAIGVLGLGLTAVSCSDDPGVTLTSSDEIAITTSRPAPPTTTT
                     TAVPTTESTVNDEAGASTTTTTTTPAPGDDPFDWTAIGPDTDEGWLDVPIDPSDPSSG
                     S"
     gene            815..>1777
                     /locus_tag="PLV68_08935"
     CDS             815..>1777
                     /locus_tag="PLV68_08935"
                     /inference="COORDINATES: protein motif:HMM:NF014680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HRE01412.1"
                     /translation="MSTASVVTLVIVAALVALAAGCVLAIAWQRRVAGLQHGFGVAGA
                     DAAERAATQAVQQAAEVVAVRQAQALAEMQTAQSASQTEALQRGLRDAVAALQVQAAS
                     ERDQAVRTALEQVAVMGRETLGSQHQAASADLAAKKDVIEARLGQIEAQVHTDLERLT
                     TLVGQLQHSTSERFGQVDSSLRAHAEITQHLTSTTQGLREALASPNARGQWGERMAED
                     ILRAAGFIEHKQYRKRTAVDGDGSGIPDFTFFMPKGQVLFMDVKFPMAAYLRYLDAST
                     DAERSTHRATFLRDVRQRVRELAKRDYAATDDRAAVDNVLLFVPN"
     assembly_gap    1778..1803
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1804..2189
                     /gene="rmuC"
                     /locus_tag="PLV68_08940"
     CDS             <1804..2189
                     /gene="rmuC"
                     /locus_tag="PLV68_08940"
                     /inference="COORDINATES: protein motif:HMM:NF014680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HRE01413.1"
                     /translation="ADPTVVDEALRQHVVICSPLTLFAFLGVIRQAFDNYVLEQTSHE
                     MLQLLGKFAQQWTKYSHQVDRVKRQFDTVQKSFDELATTRRRALERPLKDLEALRLER
                     NVAVDGELFAADVLQLEGYREGLGA"
     gene            complement(2165..3418)
                     /locus_tag="PLV68_08945"
     CDS             complement(2165..3418)
                     /locus_tag="PLV68_08945"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRE01414.1"
                     /translation="MPGLPSHAALLSDALIAVSMRRGLRDHPLLTVLGIVGLAAGIVL
                     VGLGLRRFQRRSRSLRHSLTLVALSSLLIAVTAAVLASRLMVVSNDELVAFLILLGLS
                     AACATLLGVVVSKPLHDDVRHLESTVRRIEAGDRDVRVDTGRGDELGHVGRAIDDLVE
                     RLDWLERERSVLEDERRALLSNIGHDLRSPLAALQAAIEAMIDGVARDPQRYLRSMQH
                     DVEALGSLIDDLYLLARLDAGRYDLDRTVVDLVELADGAVEALAPTAAASGIRLALVA
                     NDPVRVFASAVSLSRVFRNLIDNAIRHAPQGSTVTVAVGARADGAHVRVADEGTGFPA
                     EFRNQAFERFTRADPSRNRQSGGGGLGLAIARGLVEAHGGHIWIDDGPQSAVLFRVPL
                     ATPPGGPLVFSPPSPTRRQAPSPSR"
     assembly_gap    4022..4048
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4049..4228)
                     /locus_tag="PLV68_08950"
     CDS             complement(<4049..4228)
                     /locus_tag="PLV68_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01415.1"
                     /translation="MATSVADRHERLGRRLRNVGETRLDPSRRAAAAPYSGRMAARVL
                     IVDDEPMVREVVARYL"
     gene            4227..>4557
                     /locus_tag="PLV68_08955"
     CDS             4227..>4557
                     /locus_tag="PLV68_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01416.1"
                     /translation="MSSEAMPHGALPEPMSELAAEPQCGATAARRARRRKTFAIVGVI
                     AVLVVGGGLLYFEPWTLFIDKKVDEALPGMPSTPVATAGPSSIASAIASAAATGQPVI
                     VSMTEFIS"
BASE COUNT          736 a         1507 c         1586 g          660 t
ORIGIN      
        1 aacggaagga tggcgccgct gcgttcgagg cacgccgcgc cgaagtctgc ggccgcgtcg
       61 aggatcgcct ggcgctcggc atcgtcgtcg ggcgacgagt cgagcgcgaa gtacgggtcg
      121 tagtcgtcga tgcaatcgat ggacggctcg ctgaggccgg tgccgcgcgg atcccagccg
      181 acgatgtcga agtggtcggt cagggcggtg ccgtagatga agtcggcgga ttcggccatg
      241 aagctgccgc cgaagcccgg tccgccgggg ttgacgagca gggtgccgat ccggttctgg
      301 ggatcgttgg cccggttgcg gatgacgtac annnnnnnnn nnnnnnacga acccgatgac
      361 gggtcgctcg ggtcgatcgg gacgtcgagc caaccctcgt cggtgtccgg cccgatcgcg
      421 gtccagtcga acgggtcgtc gccgggcgcg ggtgtcgtgg tcgtggtggt ggtcgaggca
      481 ccggcctcgt cgttgacggt cgactcggtg gtgggaacgg cggtcgtggt cgtcgtcggc
      541 ggtgcgggcc gcgacgtggt gatggcgatc tcgtcggacg aggtgagcgt gaccccggga
      601 tcgtcgctgc acgacaccgc ggtcaggccg aggccgagca cgccgatcgc cggcacccta
      661 cacaccctgg cgaaccgccg accccgaagc atccgccgag cgtaccgacg cgctcgacgc
      721 actcgacagc ggacccctct caccggaggc tgtgaatcgc ctggtgagag gggatcgccc
      781 aactgtgtga caccccgcga gcaacatggg cggtatgagt accgcatctg tcgtcaccct
      841 cgtcatcgtc gccgcattgg tcgccttggc ggccggttgc gtgctggcga tcgcatggca
      901 gcgccgcgtc gccgggctgc agcacgggtt cggggtggcg ggtgccgatg ccgccgaacg
      961 ggccgcgacc caggctgttc aacaggcagc agaggtcgtg gcggtccgcc aggctcaggc
     1021 gttggccgag atgcagacgg cgcagtcggc gtcgcagacc gaggcgctgc agcggggatt
     1081 gcgcgacgcg gtggccgcgt tgcaggtgca ggcggcaagc gagcgtgacc aggccgtgcg
     1141 caccgcgttg gaacaggtgg ccgtcatggg gcgcgagacg ctcggctcgc agcaccaggc
     1201 tgcctcggcc gacctggccg ccaagaagga cgtcatcgaa gcccggctcg gccagatcga
     1261 agcgcaggtg cacaccgacc tggagcggct cacgaccttg gtcggccagc tccagcactc
     1321 gacgtccgag cggttcggtc aggtcgattc cagcctgcgc gcccacgccg agatcacgca
     1381 gcacctcacc agcaccaccc agggcctgcg cgaggcgttg gccagcccga atgcccgtgg
     1441 ccagtggggc gagcggatgg ccgaggacat cctccgtgcc gcaggcttca tcgagcacaa
     1501 gcagtaccgc aagcgcaccg cggtcgacgg tgacggcagc ggcatccccg acttcacctt
     1561 tttcatgccc aagggccagg tgttgttcat ggacgtcaag ttcccgatgg cggcgtatct
     1621 gcgctacctc gatgcatcca ccgacgccga gcgaagcacg caccgggcca cgttcttgcg
     1681 tgacgtgcgc cagcgggtgc gcgaactggc caagcgcgac tacgccgcca ccgacgaccg
     1741 ggccgcggtc gacaacgtgt tgctgttcgt cccgaacnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnaggcaga ccccaccgtg gtcgacgagg cgctgcgcca gcacgtggtg atctgctcgc
     1861 cgctgacgct cttcgcgttc ctcggcgtga tccgccaggc gttcgacaac tacgtgctgg
     1921 agcagaccag ccatgagatg ttgcaactgc tcggcaagtt cgcccagcag tggaccaagt
     1981 actcacatca ggtcgacagg gtgaagcggc aattcgacac ggtgcagaag tcgttcgacg
     2041 agttggccac cacccgccgt cgggcgctcg aacgtccgct gaaggacttg gaggcgttgc
     2101 gcctggaacg caacgtcgct gtcgacggtg agctgttcgc ggccgacgtg ttgcagttgg
     2161 agggttaccg cgaggggctc ggcgcttgac gtcgggtcgg gctgggcgga ctgaacacca
     2221 gcggaccacc gggcggtgtc gccaacggaa cgcggaacag cacggccgat tgcggtccgt
     2281 cgtcgatcca gatgtggcca ccgtgggctt cgaccagacc gcgggcgatc gccaggccca
     2341 acccgccacc gcccgactgg cggttacggc tggggtcggc acgggtgaag cgttcgaatg
     2401 cctggttgcg gaactcggcc gggaacccgg tgccctcgtc ggccacccgc acgtgagcac
     2461 cgtcggctcg ggcgccgacc gcgacggtca ccgtcgaacc ctgcggcgcg tgtcggatcg
     2521 cgttgtcgat caggttgcgg aacacccgcg acagcgacac ggcgctggcg aacacccgca
     2581 ccgggtcgtt ggccaccagc gcgaggcgga tgccggacgc tgctgccgtg ggcgccagcg
     2641 cctcgacggc gccgtcggcg agctcgacca ggtcgacgac ggtgcggtcg aggtcgtatc
     2701 ggcccgcatc gaggcgggcg agcaggtaca ggtcgtcgat cagcgacccc aacgcctcga
     2761 cgtcgtgctg catcgaacgg agataccgct gcgggtcgcg cgcgacaccg tcgatcatcg
     2821 cttcgatcgc agcctgcaac gcggccagcg gcgaccgaag gtcgtggccg atgttgctca
     2881 gcagcgcccg ccgctcgtcc tccagcaccg atcgctcgcg ctccagccag tcgagccgtt
     2941 cgaccaggtc gtcgatcgcg cggccgacat gacccaactc gtcgccacgg ccggtgtcga
     3001 cacgcacgtc gcgatcacca gcctcgatgc gacgcacggt cgattccagg tggcgcacgt
     3061 cgtcgtgcag cggcttcgac accaccacgc cgagcagggt ggcgcatgcc gcgctcagtc
     3121 cgagcaggat cagaaacgcg accagctcgt cgttggacac caccatcagg cgtgatgcca
     3181 acaccgccgc ggtcaccgcg atcagcagtg acgacagcgc caccagcgtg agcgagtgac
     3241 gcaatgagcg ggaccggcgc tgaaaccggc gcaagcccag gccgaccagc acgatgccgg
     3301 cggccagccc gacgatgccc aacacggtga gcagcgggtg gtcgcgcaag ccccgtcgca
     3361 tcgacaccgc gatgagggcg tcgctcagca acgcagcgtg actcggcagg ccaggcatcg
     3421 tcggttcctc tggttcctct ggttcctacg gttcgaagcg gtagccgacg ccgcgcacgg
     3481 tgacgagcca ccgcggctgg tcggggttgg cttccaactt ctggcgcagc cgcccggacg
     3541 gtgacggtgg ccgggtcttg gtagtcgggc gaactcgacc acacctgatc gagcagttgg
     3601 gcgcgcgaga acacctgacg cggcgagtgc atcagcagcg ccaacacgtc gaactctttc
     3661 ggcgtcaacg ccatcagctc accgtgcagg aacacctcgc ggctgccggt gtcgatcgcc
     3721 aggccgtcga actcgcgccg gttcgacgcc tcgggggtgc gcgtggtggt gcgccgcaac
     3781 accgtgcgca cccgggcggc cagctcgcgc ggcgagaacg gcttgaccac gtagtcgtcg
     3841 gcaccgagct ccaacccggt gatgcggtcg acctcgtcgg cgcgggcagt gagcaggatg
     3901 accggcacat cgctggtggt gcgcaggcga cgcagaatcg acagcccgtc gacttcgggc
     3961 agcatcacgt cgagcaccac caggtcgggc tcattgtcgt cgagccagcg ttgggcggtc
     4021 annnnnnnnn nnnnnnnnnn nnnnnnnnga gataccgggc gacgacctcg cgcaccatcg
     4081 gctcgtcgtc gacgatcaac acccgggctg ccatgcggcc agagtagggg gccgcagcgg
     4141 cgcgccggga cggatcgagc cgagtttcgc caacgtttcg caggcgtcgt ccgagccgtt
     4201 cgtgacgatc ggcaacactg gtggccatgt cgagcgaggc gatgccacac ggggcgctgc
     4261 cagaaccgat gtccgaactc gctgccgaac cgcagtgcgg tgccacggcc gcccggcgtg
     4321 cccgccgacg caagacgttc gcgatcgtcg gtgtgatcgc cgtcttggtg gtcggtggcg
     4381 gcctgctcta cttcgaaccg tggacgctgt tcatcgacaa gaaggtcgac gaggcactgc
     4441 ccggcatgcc gtcgacgcca gtggcgacgg cgggcccgag cagcatcgcc tcggcgatcg
     4501 ccagcgccgc agcgaccggt cagccagtga tcgtgtcgat gaccgagttc atcagca
//
LOCUS       DAORNF010000195         4553 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_14143_length_4553_cov_1.906847, whole genome shotgun sequence.
ACCESSION   DAORNF010000195 DAORNF010000000
VERSION     DAORNF010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4553
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14143_length_4553_cov_1.906847"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    230..340
                     /estimated_length=111
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<341..>708)
                     /locus_tag="PLV68_08960"
     CDS             complement(<341..>708)
                     /locus_tag="PLV68_08960"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01417.1"
                     /translation="LLLATNNTSRTNVGEPLPRSGRVPVLTLFGNRVSWGAVIAIVLT
                     VVVGVVLNRTVLGFKLRTLGLNRRTARRFGVSSVGIGGAALVFSGASAGLGGALWLGG
                     GAAGDRFTGAISSNLGWQGL"
     assembly_gap    709..834
                     /estimated_length=126
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<835..>1177)
                     /locus_tag="PLV68_08965"
     CDS             complement(<835..>1177)
                     /locus_tag="PLV68_08965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01418.1"
                     /translation="PVWNKHRSLLGLYLASIAVALGLCALLVSVTGGSATRVFSALLD
                     GSLRSPGAWGLTITTAAPLLVVAVGTIVAGKAGLDNIGQEGQVLLGAAGFAYVATRAD
                     LPGPLLVTVSLI"
     assembly_gap    1178..1251
                     /estimated_length=74
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1276..2859)
                     /locus_tag="PLV68_08970"
     CDS             complement(1276..2859)
                     /locus_tag="PLV68_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRE01419.1"
                     /translation="MVFDGTTPAPAVRLSAITKRYGTVVACNGVDINLAAGRVHGVLG
                     ENGAGKSTLMKILIGLVQPDEGTIEIDGATVRIADPIAASDLGIAMVHQHYSLVESMT
                     VWENVVLGEQTRLDRTRACERVADISARYGLGVDPLSVVAGLTAGQRQRVEIIKCLRR
                     EPRIIVFDEPTSMLTPAESEQLFTVLRTAVAAEGRAVALVSHKLDEILHATDEVTIMR
                     SGCVVDRLHTPDATASVLAQAMVGRPVSLRSEAAALGLIDAAVADAVNDDDAAHEADE
                     VAQAAVVLEVRGLTVADDDGHKVLDDLSIIVHAGEIVGLAGVEGNGQRPLGDVLSGLV
                     EPDSGEVLVAGVAVDPSRPGALHAARVGVIPEDRHDSGCVLGMSVAENLMLGSIGSAS
                     RGGIIDRRAIDKHARDLMREFDIAARSPHAPMSSLSGGNQQRVVLARELSGNPVVLVA
                     AQPTRGLDVGAIEYMSERLRAAADKGVGVLLISTELEEVLTLAHRICVIHEGRIVGEM
                     ARHEASIERLGLLMGGVSA"
     gene            complement(2906..4045)
                     /locus_tag="PLV68_08975"
     CDS             complement(2906..4045)
                     /locus_tag="PLV68_08975"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HRE01420.1"
                     /translation="MSNSSLRGRRRSTSLVAATAALGLLVAACGSDDKKADETTTTTA
                     APVVTDAPSTTSGAPAAQSLDTNGDGKVVIGVATPGPRNDGGYYQALVDGVTTFSKEN
                     GFADPIVVDNIKQEEAASSLDALARQNVDMIAVGAGEIADPLPELTAKYSDVIWYCNC
                     GAGFQQLPNLIQSGDDGAEMGYSAGVATGLLLKAADKDSAGFIGNNNFNFEIEAFKSF
                     ELGLKSVDDSFTATYVATGSFNDVAAATEAFNNLKAQGVGAVWPYLGGAHEPVVKLAN
                     EAGIITMSAGASNACDRTDLDYQIAVRFDAGDYLAPILEEIQAGTLKRGETRGFHVGV
                     DPQGGAVICDPTSDQAAAMKEAFDLIASGDLNADFGAIKGEAYGG"
     gene            complement(4122..>4553)
                     /locus_tag="PLV68_08980"
     CDS             complement(4122..>4553)
                     /locus_tag="PLV68_08980"
                     /inference="COORDINATES: protein motif:HMM:NF015682.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01421.1"
                     /translation="IWGEILSVAAQRQGAVGAAIDGAARDRPEIEALGFALYARDERV
                     VGPYGTAHVVAIDEPVTIMNSHGTNVHGNGINVRCGDIVVADDTGLVCLDPIEGETIV
                     ADAVRYAMGEAAVVAALNNGELLSSAYRNKQQVVQAISTVS"
BASE COUNT          705 a         1462 c         1403 g          672 t
ORIGIN      
        1 tgcccgactt ctccgcgatc cactcgccga tcgcggcgaa caggatgagg gtggcgaacc
       61 gcacggtcga ggagtacatc gtgtcggacg tgacgacatc ccacagcgag ccaccgaagg
      121 cgagcatcac gccaccgccc ttccgcggcg acggctgcgc agggcctgca cggccggtgg
      181 caccaacagc gcgagcacca gcatcgcctg gaccacgtcg gtgatccgcn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn caacccctgc cagccgaggt
      361 tggacgagat cgctccggtg aaccgatcac ccgcggcgcc accgccaagc cacaaggccc
      421 cgccgagacc ggcggacgcg ccggagaaca ccaacgccgc gccaccgatg cccaccgaag
      481 agacgccgaa gcggcgggcc gtgcgccggt tgagacccag ggtgcgcagc ttgaagccga
      541 gcactgtgcg gttcagcacg acgccgacca cgaccgtgag cacgatcgcg atcaccgcac
      601 cccacgacac ccggttgccg aacagcgtca gcacgggcac ccgacccgac cgcggcaacg
      661 gctcaccgac gttggtgcgc gacgtgttgt tcgtggccag cagcagccnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncgatca
      841 gcgagacggt gaccagcaac ggcccgggca gatcggcccg cgtcgcgacg tacgcgaacc
      901 ccgccgcgcc cagcagcacc tgaccttcct ggccgatgtt gtccagcccc gccttgccgg
      961 cgacgatggt gccgaccgcg accaccaaca acggggccgc ggtggtgatc gtcagccccc
     1021 acgcaccggg cgaacggagg ctgccgtcga gcagagcgga gaacacgcgc gtcgccgagc
     1081 cgccggtcac cgacaccagc agcgcgcaca agccgagcgc gaccgcgatc gacgcgaggt
     1141 acagcccgag cagcgaacgg tgcttgttcc acacgggnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncgccagcgg
     1261 cggggatcgc agtgctcacg cgctgactcc gcccatgagc aggccgagcc gttcgatgct
     1321 ggcctcgtgg cgggccatct caccgacgat gcgaccttcg tggatgacac agattcggtg
     1381 tgccaaggtc agaacctcct cgagttcggt cgagatcagc aacacaccga cgcccttgtc
     1441 ggccgcggca cgaagccgct cactcatgta ttcgatagct ccgacgtcga gcccgcgggt
     1501 gggctgcgcg gcgacgagca ccacggggtt gcccgacagt tcgcgggcaa gcacgacgcg
     1561 ttgttggttg ccgcccgaca gcgacgacat tggcgcgtgc ggcgagcggg cggcgatatc
     1621 aaactcgcgc atcaggtcac gcgcatgctt gtcgatcgca cgacggtcga tgatgccgcc
     1681 acggctcgcg gacccgatgc tgcccagcat caggttctcc gccacgctca tgcccagcac
     1741 gcagccggag tcgtggcggt cttcgggaat gacaccgacc cgggctgcgt gcagcgcgcc
     1801 cggccgggac gggtcgaccg ccaccccggc gacgagcact tcgccactgt cgggctcgac
     1861 caacccggac agcacatcgc caagaggccg ttgaccgttg ccctcgacac ctgccaggcc
     1921 gacgatctca ccggcgtgca cgatgatcga caagtcgtcg agcaccttgt ggccgtcgtc
     1981 gtcggcaacc gtcaggccgc gcacttccag cactaccgcg gcctgcgcaa cctcgtcggc
     2041 ttcgtgcgcg gcatcgtcgt cgttgaccgc gtctgcaacc gccgcgtcga tcaggccgag
     2101 cgcggccgct tccgatcgca acgatacggg tcgcccgacc atggcctggg ccagcacgga
     2161 cgctgtggcg tcgggcgtgt gcagacgatc gaccacacac cccgagcgca tgatcgtcac
     2221 ttcgtcggtc gcgtgcagaa tctcgtcgag cttgtgcgac accagcgcga cggcccgtcc
     2281 ttcggccgcg accgcggtgc gcaacaccgt gaacagctgc tcgctctccg ccggggtgag
     2341 catgctcgtc ggctcgtcga agacgatgat ccgcggttca cggcgtagac acttgatgat
     2401 ctcgacgcgc tggcgttgcc cggccgtgag accggccacc acggacaacg gatcgacacc
     2461 gaggccgtag cgcgcgctga tgtcggccac ccgctcgcaa gcgcgggtgc ggtcgagccg
     2521 ggtctgttcg cccaagacga cgttctccca cacggtcatc gactcgacca ggctgtagtg
     2581 ctggtgcacc atcgcgatgc ccaagtcgct ggccgcgatc gggtcggcga tgcgcacggt
     2641 ggcaccgtcg atctcgatcg tgccttcgtc gggctgcacg agcccgatca gaatcttcat
     2701 cagcgttgac ttacccgcgc cgttttcgcc gagcacacca tgcacgcggc ccgcggcgag
     2761 gttgatgtcg acgccgttgc aggcgacgac cgtgccgtaa cgcttcgtga tcgcggagag
     2821 ccgcaccgcc ggcgcggggg tggtcccgtc aaagaccacc cccgcatcag gcgtgttggc
     2881 cgcagagtct gcagcagtca ctgtgtcagc cgccgtaggc ctcgcccttg atggcaccga
     2941 agtcggcgtt caggtcgcca gaggcgatca ggtcgaacgc ttccttcatc gccgcggcct
     3001 gatccgaggt cggatcacag atgaccgcgc cgccctgcgg gtcgacaccg acgtgaaagc
     3061 cacgggtctc gccgcgcttg agcgtgccag cctggatctc ttccaagatc ggggccaggt
     3121 agtcgccggc gtcgaagcgc acggcaatct ggtagtcgag gtcggtgcgg tcgcacgcgt
     3181 tcgacgcacc agcgctcatc gtgatgatcc ccgcctcgtt ggccagcttg accaccggtt
     3241 cgtgagcgcc accgaggtac ggccacaccg cgccgacgcc ctgagccttg aggttgttga
     3301 aggcctcggt cgccgcggca acgtcgttga acgagccggt cgcgacgtag gtcgccgtga
     3361 acgaatcgtc gaccgacttc aggccgagct cgaaggactt gaacgcctcg atctcgaagt
     3421 tgaagttgtt gttgccgatg aacccggcac tgtccttgtc cgcagccttg agcagcagac
     3481 cggtggcgac acccgccgag tagcccatct cggcaccgtc gtcacccgac tggatcaggt
     3541 tcggaagctg ctggaagcct gcgccgcagt tgcagtacca gatcacatcc gagtacttgg
     3601 cggtcagttc gggcagcggg tcggcgatct cgccagcgcc gacggcgatc atgtcgacgt
     3661 tctggcgggc gagggcgtcg agcgagctgg ccgcctcttc ctgcttgatg ttgtcgacga
     3721 cgatcgggtc ggcgaacccg ttctccttcg agaacgtggt gacgccgtcg accagtgcct
     3781 ggtagtagcc gccgtcgttg cgcgggccgg gcgtggccac accgatgacg accttgccgt
     3841 cgccgttggt gtcgagcgac tgggccgcgg gggccccact ggtggtgctc ggcgcgtcgg
     3901 tgacgaccgg agcggcagtc gtggtcgtgg tttcgtcggc cttcttgtcg tcactgccac
     3961 atgcagccac gagcaagccg agtgcagcgg ttgctgcaac cagcgacgtc gagcggcgtc
     4021 ggccgcgaag tgaagagttg ctcattcgta tcccccttgg atgcggtatt gcttggtttg
     4081 gttgtgggtt gtgaggttgc tggttggtac tcgtacggcg atcaggacac ggtgctgatc
     4141 gcctgcacga cttgttgctt gttgcgatag gccgacgaga gcaactcgcc gttgttgagg
     4201 gcggcgacca cggccgcttc ccccattgca tagcggacgg cgtcggcgac gatggtctca
     4261 ccctcgatcg ggtcgaggca caccaggccg gtgtcgtccg cgacgacgat gtcgccgcag
     4321 cgaacattga tgccgttgcc gtggacattc gtgccgtggg aattcatgat ggtgaccggc
     4381 tcgtcgatcg ccacgacgtg ggcggtgccg tagggtccca ccacccgttc gtcgcgggca
     4441 tagagcgcaa agcccaaggc ctcgatttcg ggccggtcac gggctgcccc gtcgatggcc
     4501 gcgccgacgg caccttgccg ctgcgcggcg accgagagga tctcgcccca gat
//
LOCUS       DAORNF010000196         4552 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_14148_length_4552_cov_1.855237, whole genome shotgun sequence.
ACCESSION   DAORNF010000196 DAORNF010000000
VERSION     DAORNF010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4552
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14148_length_4552_cov_1.855237"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            56..>360
                     /locus_tag="PLV68_08985"
     CDS             56..>360
                     /locus_tag="PLV68_08985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01422.1"
                     /translation="MEFFIASAQIPLFTAAARIGFDTPDFTPTTYDALHPGTWQQAGR
                     LADMSTDGVEASMCYPNIVPRFCGQTFLERGDRDTSLECVRLYNDWMLDEWCAGAGHG
                     "
     assembly_gap    361..418
                     /estimated_length=58
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <419..>792
                     /locus_tag="PLV68_08990"
     CDS             <419..>792
                     /locus_tag="PLV68_08990"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRE01423.1"
                     /translation="DKGTVAVAFSENPYQLGLPSLHSGAWDPFLRTAADAGVTVCMHI
                     GSSSHMPSTSPDAPHIISSLTHFSVTAGSLLDFIFSGTLDRIPNLKLFFAESQAGWLP
                     FVLEQADQLWALREGSSMSVDL"
     assembly_gap    793..820
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <821..1053
                     /locus_tag="PLV68_08995"
     CDS             <821..1053
                     /locus_tag="PLV68_08995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01424.1"
                     /translation="TSIFHDDAALRNRDTIGVRQMCFETDYPHSVTTFPHSHPSALEA
                     CRKAGLDDGEIYEVMRGTAIRAFDLGRVGIAA"
     assembly_gap    1208..1250
                     /estimated_length=43
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1251..4332
                     /locus_tag="PLV68_09000"
     CDS             <1251..4332
                     /locus_tag="PLV68_09000"
                     /inference="COORDINATES: protein motif:HMM:NF012361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HRE01425.1"
                     /translation="NADGPKFSFIDGPVTANKSLAVHTAWGRTLKDVFQRYKALNGFH
                     QRYQNGFDCQGLWIEVGVEKALGLNSKREIEEYGLAEFANQCRAVVEQSSADLTAGSI
                     RLGQWMDWGNDYFTFSDTNIEYIWKFLSIVNERGWLAKGHRATEWCPRCGTSISAHEL
                     AGSYIDKDDPSLYVRFPLLDRPGEALVIWTTTPWTLPANVAAAVKPELTYGRRANGDW
                     LAVDSPGATALRGVQGVDADDATGGFVETKLGSELIGWRYEGPFDMFAPGAAAGHRVI
                     PWDAVSTDDGTGVVHIAPGCGSEDFELGKEHGLVMIQPVDEIGRFYPDFGWLAGQSTV
                     EAKDAILADLTDRNRLLLAGTVHHRFPECWRCHTPLIFRISDDWFISVVDLRQPMRDA
                     NAAVEWTPAYMGKRMDDWLVNMGDISRRRYYGLPLPFYPCSCGHLTVIGSRQQLAELA
                     VRPDGASADFDPLADVQELRRPWIDAVRIHCPACSQPVSRIEEVGDVWLDAGIVPFST
                     LGWQNDTWVPGGYGTGAAKGLTTADLPDHSYWQEWFPADWVSEMREQIRLWFYSQLFM
                     SVVLTGRAPYDRVLGYEKMLDETGREMHGSWGNQIDAPDAFQRMGADVMRWQFCQQPP
                     SQDLLFGFKPAYEIQRKMLTLWNSVAFFVQYANVAGFTPSYDALTADGCDAAAGDTDA
                     HSMDRWLHALTQRFLATATEGYEAYLSVNVLRAFEQYVDDVSNWYIRRSRRRFWNGDA
                     DALNALWVALVQGLRAVAPIMPFVTEQLWQQLVRDVCPDAPPSVFLAGWPTAQATDDT
                     LLAEVATMRTVIELGRRARSAAKLPLRQPLSMLAVDLSHGADAIDLGAYADEIAEELR
                     VKQVAFEPIEATQIKVRPNLKTLGPRLGSDVNTVRQALAAGEFEMLPDGSCVVAGHTL
                     PPADLLVERTDKEGWAVATDDANTITVAFETTLTDELRTERRVYDLIHDVNGRRKETG
                     LEITDRIALSLAAADADLVAHADWIKAETLTVDLTVGTTDDFTIAKAVAADQGA"
     gene            4337..>4552
                     /locus_tag="PLV68_09005"
     CDS             4337..>4552
                     /locus_tag="PLV68_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01426.1"
                     /translation="MTDTANPKPALDKPALDKPALDKPALAKPTLEGLEQKWDAAWEA
                     AGTYRFDRSKTRAEVYSIDTPPPTVSGE"
BASE COUNT          721 a         1562 c         1433 g          707 t
ORIGIN      
        1 ctcatcaccg acttcgggca ggttgtagta agcgaccttt tgccgccacc cgccggtgga
       61 attcttcatt gccagcgcgc agatcccgct gttcacggcc gcggcgcgga tcgggttcga
      121 cacgcccgac ttcacaccga ccacctacga cgcgctccac cctggcacgt ggcagcaggc
      181 cgggcgcctc gccgacatga gcaccgacgg ggtcgaggcc tcgatgtgct accccaacat
      241 cgtcccacgc ttttgcgggc agaccttctt ggagcgcggc gatcgtgaca cgtcgctcga
      301 atgcgtgcgc ctctacaacg actggatgct cgacgaatgg tgcgccggtg ccggccacgg
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncg
      421 acaagggcac cgtcgcggtc gcgttctccg agaaccccta ccaactcggc ctgccgtcgc
      481 tgcactccgg agcgtgggac ccgtttctac gcaccgcggc cgatgccggg gtgaccgtgt
      541 gcatgcacat cggctccagc tcgcacatgc cctctacgtc gccggacgcg ccgcacatca
      601 tctcgtcgct gacccacttc tcggtcacgg ccggatcgct gctcgacttc atcttctcgg
      661 gcaccctcga ccgcattccc aacctcaagc tgttcttcgc cgagagccag gcgggctggc
      721 tgccgttcgt gctcgagcag gccgatcagc tgtgggcgtt gcgcgagggc tcgtcgatga
      781 gcgtcgacct tcnnnnnnnn nnnnnnnnnn nnnnnnnnnn tcacgtcgat cttccacgac
      841 gacgccgcgc tgcgcaaccg cgacacgatc ggcgtccgcc agatgtgctt cgagaccgac
      901 tatccgcact cggtgacgac gttcccccac tcgcacccca gcgcgctcga agcctgccgc
      961 aaggccgggc tggacgacgg cgagatctac gaggtcatgc gcggtacggc catccgggcc
     1021 ttcgatctgg gccgggtcgg catcgctgcg tgattgcccc cgggctggtg cctgggcgtg
     1081 ccataccggg catgccagat cagccatgcc agatcggcca tgggggtcac gggggtctgg
     1141 ccgtagaatc ggcgctctat gttcgcggcg cttcctgaca aaccggatca caactcgctc
     1201 gcgttgcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gaacgccgac
     1261 ggccccaagt tcagcttcat cgacggtccg gtcacggcca acaagtcgct cgccgtgcac
     1321 acggcgtggg gccgcacgct gaaggacgtg ttccagcgct acaaggcgct caacggcttc
     1381 caccagcgct accagaacgg cttcgactgc cagggtctgt ggatcgaggt cggcgtcgag
     1441 aaggcgctcg ggctcaactc caagcgggag atcgaagagt acggactggc cgagttcgcc
     1501 aaccagtgcc gggccgtggt cgagcagtcg tcggccgacc tcaccgccgg gtcgatccgt
     1561 ctcggccaat ggatggactg gggcaacgac tacttcacgt tctccgacac caacatcgag
     1621 tacatctgga agttcctgtc catcgtcaac gagcgggggt ggctggccaa gggccatcgg
     1681 gccaccgagt ggtgcccgcg ctgcggcacc tcgatcagcg cccacgaact ggcgggcagc
     1741 tacatcgaca aggacgatcc gtcgctgtac gtgcgcttcc cgctgctcga tcgccccggc
     1801 gaggcgttgg tcatctggac caccacgccg tggacgctgc cggccaacgt ggccgcggcg
     1861 gtcaagcccg agctcaccta cggccgccgg gccaacggcg actggttggc ggtcgactcg
     1921 cctggcgcga ccgcgttgcg cggcgtgcag ggcgtcgatg ccgacgacgc gaccggcggc
     1981 ttcgtcgaga ccaagctggg ctctgagctg atcgggtggc gctacgaggg cccgttcgac
     2041 atgttcgccc ccggcgcggc ggccgggcac cgggtcatcc cgtgggatgc ggtgtccacc
     2101 gacgacggca ccggcgtggt ccacatcgcc cctggctgcg gctccgaaga cttcgagctg
     2161 ggcaaggagc atggcctggt catgatccag ccggtcgacg agatcggccg cttctatccc
     2221 gacttcggct ggctcgccgg gcagtcgacc gtcgaggcca aggacgcgat cctggccgac
     2281 ctcaccgacc gcaaccggtt gctgctggcg ggcacggtcc accaccgctt ccccgagtgc
     2341 tggcgctgcc acacgccgct gatcttccgc atctccgacg actggttcat ctccgtggtc
     2401 gacttgcgcc aacccatgcg cgacgccaac gcggcggtcg agtggacgcc cgcgtacatg
     2461 ggcaagcgga tggacgactg gctcgtcaac atgggcgaca tcagccgccg ccgctactac
     2521 ggcctgccgt tgccgttcta cccctgctcg tgcggtcacc tcaccgtgat cgggtcgcgc
     2581 cagcaactgg ccgagcttgc cgttcgtccg gatggggcgt ccgccgactt cgatccgttg
     2641 gccgacgtgc aagaactacg ccgcccgtgg atcgacgcgg tgcgcatcca ctgcccggcg
     2701 tgttcccagc cggtgtcgcg catcgaagag gtcggcgacg tatggctcga cgcgggcatc
     2761 gtgccgttca gcacgctcgg ttggcagaac gacacgtggg tgccgggcgg ctacggcacc
     2821 ggcgcggcca agggcctgac caccgccgat ctacccgacc acagctactg gcaagagtgg
     2881 ttcccggccg actgggtcag cgagatgcgc gagcagatcc ggctgtggtt ctactcgcag
     2941 ctgttcatgt cggtggtgct caccggccgt gcgccgtacg accgggtgct cggctacgag
     3001 aagatgctcg acgagaccgg ccgcgagatg cacggctcgt ggggcaacca gatcgacgcg
     3061 cccgacgcct tccagcggat gggcgccgat gtgatgcggt ggcagttctg ccagcaacca
     3121 ccgtcgcaag acctgctgtt cggcttcaag cccgcgtacg agatccagcg caagatgctg
     3181 accctgtgga acagcgtcgc gttcttcgtc cagtacgcca acgtcgccgg attcacgccg
     3241 agctatgacg cgctcaccgc cgatgggtgt gatgcagctg caggcgacac cgatgcgcac
     3301 tcgatggacc gctggctgca cgccctgacc cagcgcttcc tcgccaccgc caccgagggc
     3361 tacgaggcct acctcagcgt caatgtgctg cgcgcgttcg agcagtacgt cgacgacgta
     3421 tccaactggt acatccgccg cagccgccgc cggttctgga acggcgacgc cgatgcgctc
     3481 aacgcgctgt gggtcgcgct ggtgcagggc ctgcgcgccg tcgcgccgat catgccgttc
     3541 gtcaccgagc agctgtggca gcaactcgtg cgcgacgtgt gccccgacgc cccgccatcg
     3601 gtgttcctcg ccggatggcc caccgcgcag gcgaccgacg acacgctgct ggccgaagtg
     3661 gccacgatgc gcacggtgat cgaactcggt cgccgggctc gctccgcggc caagctgccg
     3721 ttgcgtcagc cgctcagcat gctggctgtc gacttgtcgc atggtgccga tgccatcgat
     3781 ctgggggcgt atgcggacga gatcgcggag gagctgcgcg tcaagcaggt ggcgttcgaa
     3841 ccgatcgagg ccacccagat caaggtccgt cccaacctca agacgttggg gccgcgcctg
     3901 ggcagcgacg tcaacaccgt gcgtcaggcg ctggcggcag gcgagttcga gatgctgccc
     3961 gacggtagct gcgtggtcgc tgggcacacg ttgccgcccg cggacctgct ggtcgagcgg
     4021 acggacaagg agggttgggc cgtcgccacc gacgatgcca acaccatcac tgtcgcgttc
     4081 gagacgaccc tcaccgacga gctgcgcacc gagcgccgcg tctacgacct gatccacgac
     4141 gtcaacggcc gccgcaagga gaccgggctc gagatcaccg atcgcatcgc gctgagcctc
     4201 gccgcggccg acgccgatct ggtcgctcat gccgactgga tcaaggccga gacgctcacc
     4261 gtcgatctca ccgtcggcac caccgacgac ttcaccatcg ccaaggccgt cgcggccgac
     4321 cagggagcgt gaccccgtga ccgacacagc gaacccgaag cccgcgctgg acaaacccgc
     4381 gctggacaaa cccgcgctgg acaaacccgc gcttgccaaa cccacgcttg agggcttgga
     4441 gcagaagtgg gacgcggcgt gggaagcggc tggaacctat cgcttcgacc gcagcaagac
     4501 ccgcgccgag gtctactcca tcgacacccc gccgcccacg gtcagcggcg ag
//
LOCUS       DAORNF010000197         4544 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_14177_length_4544_cov_1.823791, whole genome shotgun sequence.
ACCESSION   DAORNF010000197 DAORNF010000000
VERSION     DAORNF010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4544
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14177_length_4544_cov_1.823791"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..952)
                     /locus_tag="PLV68_09010"
     CDS             complement(<1..952)
                     /locus_tag="PLV68_09010"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRE01427.1"
                     /translation="MDFTTDEVHQGIREGARAVCSRFPDSYWREHDEQHEFPWDFYEA
                     LAADGWIGVAIPEQYGGGGQGITEASIILEEIAASGAAMNGCSAVHLSIFGMNPIVKH
                     GSEELKSRYLPEVAAGRLHVAFGVTEPNSGTDTTSIQTRAVRDGDNYIVNGRKVWTTK
                     ATLCDKVLLLVRTTPLAECTRRTDGMTLLFADLQVPEVTITPISKTGRNAVVSCETVY
                     DDLVVPVSHRVGEEGRGFEYLLDGLNPERILIASEALGIGKVALKKAVGYANDRVVFG
                     RPIGRNQGLAFPLADAHARLSAAELMIRQASWRYDQGLSCG"
     gene            1079..1543
                     /locus_tag="PLV68_09015"
     CDS             1079..1543
                     /locus_tag="PLV68_09015"
                     /inference="COORDINATES: protein motif:HMM:NF013722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HRE01428.1"
                     /translation="MAGLWLEELEPGYTVHHAVRRTVTEADNTLFSVMTMNPQPLHLD
                     AEFSSHTEYGERLVNSLFTLGLVIGISVYELTLGTTVANLSFDAVEFPAPVFHGDTIH
                     VESQVVAARASRSRPDAGIVTFEHRGYNQHDVLVARCVRNAMMRRRPATVSA"
     gene            1554..2429
                     /locus_tag="PLV68_09020"
     CDS             1554..2429
                     /locus_tag="PLV68_09020"
                     /inference="COORDINATES: protein motif:HMM:NF015297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HRE01429.1"
                     /translation="MRLRSMLFAPANRGDFVAKLPRSGADSLVADLEDAVPLASKAGA
                     RAALPEIIAGVPARGADQLLLVRVNAVTSPLFALDVAAAARCPIDGIVVPKLERAEHV
                     DQIRTALAARNVVDWVIVAGIETARGVHLAEEVLDAGVDGCYFGAEDFVTDMGGRRTA
                     GNAEVLYARSRVALVARLAGVPALDQVVTDLQDADRFRADAHEGRNIGYSGKLCVHPR
                     QVALVHECYAVSAEELAWAHRVLAAEALAATDGRGVFEVDGQMIDEPLILRARRLIAA
                     AGSHSPEDVPATTHP"
     gene            2469..>2649
                     /locus_tag="PLV68_09025"
     CDS             2469..>2649
                     /locus_tag="PLV68_09025"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01430.1"
                     /translation="METNGLVGLTVDGNVAIVALTRADRANSMDGAVFTALVEVLDEV
                     RGRDDIRAVVLCGDGR"
     assembly_gap    2650..2727
                     /estimated_length=78
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2728..3188
                     /locus_tag="PLV68_09030"
     CDS             <2728..3188
                     /locus_tag="PLV68_09030"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HRE01431.1"
                     /translation="AYGVTERLLDLPLPIVCAVQGRCAGAALAMVLSCDLRIATESAV
                     FSLDFVRLGLSPDMGVCWLLGSAVGIGRALDFALSGELMDAQRALDWGIVTRVVPDGG
                     ERHASTPSRRSSSPARSASTRQPGWPPSGVWSAPLRSSAVRKRSRTRSRR"
     gene            3265..4338
                     /locus_tag="PLV68_09035"
     CDS             3265..4338
                     /locus_tag="PLV68_09035"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HRE01432.1"
                     /translation="MAVPTSRDPAVTELQLARFLQASRPEMSNVSVSLLPSPRSTGFS
                     SETLLFDATYTDPAGAVMVAPCVARIRPADYTLYQDHDLESQWRVIDALSRHTDVPVP
                     RILAHDAIEGTWLGQQCFVMDRIDGAAAADLPPYTVKGWLKDSSAAEQRQVYVGGLEV
                     LAGVHAADWRGLGLGFLQESTANPVGLQQQLEHDERFLGWVTAGREFPELQRASAWLR
                     ANLPDDGELLLSWGDARLGNMLFRDHRPVAVLDWEMVTLAAPGADLGWWLVFNLIHSV
                     GIDRPSLPGIPTDDEAVGIYESVAGRTVTDLIFYEVRAALRAALLLIRFSDAMVENGS
                     LPPEAAARPYTPAVKTLTYLLAR"
BASE COUNT          685 a         1488 c         1472 g          821 t
ORIGIN      
        1 caccgcagga cagaccctgg tcgtagcgcc acgacgcttg gcggatcatc agctcggccg
       61 cgctgaggcg ggcatgcgca tccgcgagtg gaaacgccag gccctggttg cgcccgatcg
      121 gacggccgaa cacgacccgg tcgttggcgt agccgacggc cttcttcagc gcgaccttgc
      181 cgatgcccag tgcctcggac gcgatcagga ttcgctccgg gttgagaccg tcgagcaggt
      241 actcgaaccc acggccttct tcaccgaccc gatggcttac tggcacgacg agatcgtcgt
      301 agaccgtttc gcaggagacc accgcgttgc gtcccgtctt ggagatcggc gtgatcgtca
      361 cctcggggac ttgcaggtcg gcgaagagca aggtcatccc gtcggtacgc cgcgtgcact
      421 ccgccagcgg cgtggttcgc acgagcagta gcaccttgtc gcacaaagtc gccttcgtcg
      481 tccagacctt gcggccgttg acgatgtagt tgtcgccgtc gcgcacagcc ctggtctgga
      541 tcgaggtcgt gtccgtgccc gagttgggct cggtcacacc gaaggccacg tgcaaccggc
      601 ccgcagcgac ttcgggcagg tagcgcgact tgagttcctc gctgccgtgc ttgacgatcg
      661 ggttcatccc gaagatcgac agatggacgg cagaacaacc gttcatggcc gccccggagg
      721 cggcgatctc ttcgaggatg atcgatgctt cggtgatgcc ctgcccgccg ccgccgtact
      781 gctcgggtat cgccaccccg atccagccgt ctgcggccag cgcctcgtag aagtcccatg
      841 ggaactcgtg ctgctcgtcg tgctcgcgcc agtacgagtc ggggaacctg ctgcagacgg
      901 cacgtgcgcc ttcccgaatg ccctggtgca cctcgtcggt ggtgaagtcc attcgtcgtc
      961 ccttctgcgc ggcgtcgcgg tcaatgatag aattctaccg gtcggttggt cgcatgtctg
     1021 tgtcgatgcg gtcggtgcgg cttgcgttgg gtcgcgtgtg gatggggagg atttcacgat
     1081 ggcaggtctg tggttggagg agctcgaacc tggctacacg gtgcaccatg cggtgcgccg
     1141 cacggtcacc gaggccgaca acacgctgtt cagtgtgatg acgatgaatc cgcagccgtt
     1201 gcacctcgat gccgagttct ccagccacac cgagtacgga gagcgcctcg tcaacagcct
     1261 gttcaccctt ggcctggtca tcgggatctc ggtctatgag ctgacactag gcacgaccgt
     1321 tgccaacctc agcttcgatg ccgtcgagtt tccggcaccg gtgtttcacg gtgacaccat
     1381 ccacgtcgag agtcaggtcg tcgccgcgag agcctcccgg tcgcggccgg atgccggcat
     1441 cgtcaccttc gagcatcgtg gctacaacca gcacgacgtg ctcgtggccc gttgcgtgcg
     1501 caacgcaatg atgcgccgcc ggccagcgac ggtgtcggcg taacggagcc atgatgcgac
     1561 tgcgcagcat gctcttcgcc ccggccaatc ggggtgactt cgtcgccaag ctgcctcgtt
     1621 cgggtgcgga ctcgctcgtc gcggatctgg aggatgccgt gccgttggcc agcaaggcgg
     1681 gtgcgcgggc ggctctgccc gagatcatcg ccggggtccc ggcacgagga gcagaccagt
     1741 tgctcctggt gcgggtcaac gccgtgacct cgccgctgtt cgctctggat gttgccgctg
     1801 ccgcgcgctg cccgatcgac gggatcgtgg tgccgaagct cgagcgggcg gaacacgtgg
     1861 accagatccg taccgctctg gcggcccgca atgttgtgga ttgggtgatc gtcgccggca
     1921 tcgagaccgc cagaggagtg catctcgccg aggaggtact cgatgccgga gtcgacggct
     1981 gctacttcgg cgccgaggac ttcgtcaccg acatgggtgg ccggcgcacc gcgggcaacg
     2041 ccgaggtgtt gtatgcgcgt tcgcgagtgg cgctcgtggc ccgcctcgct ggtgtaccag
     2101 cgctcgacca ggtcgtgacc gatctgcagg atgccgatcg attccgcgcc gatgctcacg
     2161 agggtcgcaa catcggctac tcgggcaagc tctgcgtgca tccgcgccaa gtagcgctgg
     2221 tgcacgagtg ctatgcggta agcgccgagg agctcgcgtg ggcacatcgc gtgctcgccg
     2281 cggaggcgct tgccgccacc gatggcagag gcgtgttcga ggtcgacggg cagatgatcg
     2341 acgaacccct gatcttgcgt gctcgccgtc tcatcgccgc agccggctca cattcccccg
     2401 aggacgtccc ggcgacgaca catccctgac ggcacaccgg tgtctcccag tcaacgaatg
     2461 gagtagcaat ggaaacgaat ggtctggtcg ggctgaccgt cgacggcaac gtagcgatcg
     2521 tcgcgctgac ccgcgccgac cgagcgaact cgatggatgg cgccgtgttc actgccttgg
     2581 tcgaggtcct cgacgaggtc cgtggccgcg acgacatccg tgccgtggtg ctgtgcggcg
     2641 atggacgtcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnatg cctacggtgt caccgaacgc ctgctcgatc
     2761 tgccgctgcc gatcgtctgt gccgtgcagg gtcgctgcgc tggcgctgca ctggccatgg
     2821 tgctcagctg cgacctgcgc atcgctaccg agtcggcggt gttcagcctc gacttcgttc
     2881 gtctcggtct gtctccggac atgggtgtgt gttggttgtt gggctcggcc gtcggcatcg
     2941 gccgcgcgtt ggacttcgcc ctcagcggtg aactgatgga tgcgcagcgt gcgctcgact
     3001 gggggatcgt cacccgcgtg gtgcccgacg gtggtgaacg acacgcatcg acgccttcac
     3061 gacggtcgag ctcgcccgcc agatcagcca gcacccgcca gccgggatgg ccgccatccg
     3121 gcgtttggtc cgcaccgctc cgttcgtcag ccgtgaggaa gcgttcgagg acgagatcgc
     3181 gacgatgacg caactcacgg ccactccaga tgcgcgggcg cggttggaag cctttcggca
     3241 acgcgctcgt gcggcgaagc gctgatggcc gtccccacgt cgcgcgaccc agccgtcacc
     3301 gaactgcagc tggcccgctt cctacaggct tcgcgccccg agatgagcaa tgtcagcgtc
     3361 agcctgctcc cgtcgccgcg gagcaccggg ttctccagcg agacgttgct cttcgacgcc
     3421 acctacacgg acccggctgg cgccgtgatg gtggctccgt gtgtggcgcg tatccgccca
     3481 gcggactaca cgctgtatca agaccacgac ctggagtcgc agtggcgggt catcgacgcc
     3541 ctgtcgcggc acaccgacgt cccggtgccg cgcatccttg cccacgacgc gatcgagggc
     3601 acctggctcg gtcagcagtg cttcgtcatg gatcgcatcg acggtgccgc ggccgccgac
     3661 cttccgccgt acaccgtcaa gggatggctg aaggactcgt cggccgccga gcagcgccag
     3721 gtgtatgtgg gtgggttgga ggtgctggcc ggggtccacg ccgccgattg gcgtggcctg
     3781 ggcctgggct ttctgcagga gtcgacagcc aacccggtcg gactccagca gcagctcgaa
     3841 cacgacgagc gattcctcgg ctgggtgacg gctggacgcg agttcccaga gttgcagcgc
     3901 gcatcggcct ggctgcgtgc caacctgccc gacgacggcg agctgctcct ctcgtggggt
     3961 gacgcacggc ttggcaacat gctgttccgt gatcatcggc cggttgccgt gctcgattgg
     4021 gagatggtca cgctggcagc tcccggtgcg gatctcggtt ggtggctggt cttcaacctg
     4081 atccacagcg tcggcatcga ccgcccgtcg ctgcccggaa tccccaccga cgacgaagct
     4141 gtcggcatct acgagtccgt cgccggacgc acggtcaccg acctcatctt ctacgaggtc
     4201 cgcgccgcgc tgcgggcggc actgttgctc attcgcttca gcgatgcgat ggtggagaac
     4261 ggttcgctgc ccccggaggc ggccgcacgg ccgtacaccc cggccgtcaa gaccttgacc
     4321 tacctgctcg cccgctgaag ccacgaccgg tccgcaccat ccccgaggag atccgatgct
     4381 gtcgccactc gacgacttcc cgttccatca gacgccgcag ccgatcctgg tcgtcggcac
     4441 cagcgatcgc aacttctacg accgctacta cttcaacctc ttcaaccgcg accgcgagct
     4501 gttcgtaacg gccgggatcg ggcactaccc gaaccttggc gtcg
//
LOCUS       DAORNF010000198         4528 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_14242_length_4528_cov_1.480662, whole genome shotgun sequence.
ACCESSION   DAORNF010000198 DAORNF010000000
VERSION     DAORNF010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4528
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14242_length_4528_cov_1.480662"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            62..>384
                     /locus_tag="PLV68_09040"
     CDS             62..>384
                     /locus_tag="PLV68_09040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01433.1"
                     /translation="MFDRDVLGLAPNEYVGGVTADGLLIVTQRASDCDALVGGAVPTT
                     TSTSTWTSSGSGVDGEAVTVTTASPATPSVPDDSVAVATTVPGVSFGCFDGPPETTPT
                     TSVSET"
     assembly_gap    385..403
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <404..>1057
                     /locus_tag="PLV68_09045"
     CDS             <404..>1057
                     /locus_tag="PLV68_09045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01434.1"
                     /translation="ISDGSVPPDTGPPDTSPNSSGVWAYATGVRTWAELGIDPDLGAR
                     LNTPGVVYVNPRGGSGFSEGTFPTYPDTGNGAAGNAATSVSSIAAASVESGFILATTI
                     DVTDDTAATVDSGAVVSTSADGVAWTTAQLTMRTVNGVGQLADGTYVVVGTGVRGDLW
                     SSSPVIVARSADGVTWSESSFDALLTEADGASAWWDLQRVVIGPEAIAVVAAVNTDP"
     assembly_gap    1058..1072
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1073..>1418
                     /locus_tag="PLV68_09050"
     CDS             <1073..>1418
                     /locus_tag="PLV68_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01435.1"
                     /translation="HDGITVSMVDRDGTIEFHDRSGELLGTYDPRTAASGGGAAGGNV
                     QVDTDGTPMLIDDDGTVIYRVTDKDYESFSNMVTWPSVEYSLAVLYSTDGLAWSRDDV
                     DEIGDVGSVSPGG"
     assembly_gap    1419..1496
                     /estimated_length=78
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1584..2063
                     /locus_tag="PLV68_09055"
     CDS             1584..2063
                     /locus_tag="PLV68_09055"
                     /inference="COORDINATES: protein motif:HMM:NF012436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dps family protein"
                     /protein_id="HRE01436.1"
                     /translation="MSTPSINIGISESDRAAIAEGLGKLLADTYTLYLTTHNFHWNVT
                     GPMFNTLHTMFMTQYTELWNAVDPIAERIRSLGHPAPGSYAQFGSLSSIDDAPADPPN
                     AMDMVRILVNGHESVARTARAVFPLADAASDEPTADLLTQRLTVHEQTAWMLRSLLE"
     gene            2099..2296
                     /locus_tag="PLV68_09060"
     CDS             2099..2296
                     /locus_tag="PLV68_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01437.1"
                     /translation="MKAPDAFIAPVDAGVSIFTNIDTNIDTNVVIDVSGYITPQPLHC
                     LTRQPTARKLLDSSTTGTLTT"
     assembly_gap    2392..2429
                     /estimated_length=38
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2430..>2828
                     /locus_tag="PLV68_09065"
     CDS             <2430..>2828
                     /locus_tag="PLV68_09065"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HRE01438.1"
                     /translation="LQDIPAGLGGVTPRAILMVSGHWETTDFTVQTHPNPPMLYDYGG
                     FPEFTYHVQYPAPGAPDVAARAADLLRAAGWPVGEDAERGFDHGVFAPLVVAYPDADV
                     PVVQLSIKRSFDPAEHLAAGRALAPLRDEG"
     assembly_gap    2829..2998
                     /estimated_length=170
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3222..3231
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3232..3669)
                     /locus_tag="PLV68_09070"
     CDS             complement(<3232..3669)
                     /locus_tag="PLV68_09070"
                     /inference="COORDINATES: protein motif:HMM:NF020496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HRE01439.1"
                     /translation="MATFRTRLWAAGGNNVAIVVPEEVVLGFNRGKRVPVVVTIDGAY
                     SYRNTIASMGGQYLISFNAATRKATGRGAGDEVEVTLEADEQPRVVEVPEPLAAALAE
                     DPQAARAWAALSYSHQRQHAEAITSAKTDETRARRVAKTIEALT"
     gene            complement(3719..>4157)
                     /locus_tag="PLV68_09075"
     CDS             complement(3719..>4157)
                     /locus_tag="PLV68_09075"
                     /inference="COORDINATES: protein motif:HMM:NF019614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HRE01440.1"
                     /translation="VASVLASGNIVFGTAETDAVALEAQIQAALNGDLGIPGQTIVRT
                     REQLQRLWASDPFAGLTHQRGTYLTATFIKDGDPPLPLPTSDSELFRVVGYDADARAV
                     LAVTDNSIPGTTPDFMTWLEQACSKNITTRTWLTIQRVLTKFP"
     assembly_gap    4158..4182
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4283..>4528)
                     /locus_tag="PLV68_09080"
     CDS             complement(4283..>4528)
                     /locus_tag="PLV68_09080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01441.1"
                     /translation="HEVQLIRELGTANIGMGLAGLFALVSDWALPAGLAGGVFLLIAG
                     VLHLPKQGKNAQETLATATDLIVGLAVVGLAAYVLAT"
BASE COUNT          670 a         1486 c         1330 g          687 t
ORIGIN      
        1 gaactggcgc actgtggccg cggggccggc gggggtcatc gcggtcgcgt ccatgtcgcc
       61 ggtgttcgat cgggacgtgc tcggtctggc cccgaacgag tacgtcgggg gcgtcaccgc
      121 cgacggcctg ctgatcgtca cccagcgggc ctccgactgc gacgcgctcg tcggaggagc
      181 cgtgccgacc accacgtcga ccagcacatg gacgagcagc gggtccggag tcgacggcga
      241 ggcggtgacc gtcaccaccg cctctccggc cacaccgagc gtgcccgacg acagcgtcgc
      301 cgtcgccacg accgtgccgg gcgtcagctt cggttgcttc gacggcccgc ccgagaccac
      361 accgaccacc agtgtctccg aaacnnnnnn nnnnnnnnnn nnnccatctc cgacggctcg
      421 gttccgccgg acaccggccc gccagacacc agccccaaca gctctggtgt gtgggcatac
      481 gcgacgggtg tgcgcacgtg ggccgagttg ggcatcgatc ccgatctcgg tgcccgcctc
      541 aacacgcccg gcgtggtcta cgtcaacccg cggggcggct ccgggttcag cgaaggtacg
      601 ttcccgacct accccgacac cggcaacggg gcggctggca atgcggcaac gtcggtatcc
      661 agcatcgccg cggcctcggt cgagtcgggg ttcatcctcg ccaccaccat cgacgtcacc
      721 gacgacaccg cagcgacggt cgactcgggt gccgtggtct cgaccagcgc cgacggtgtc
      781 gcctggacca cggctcagct caccatgcgc acggtcaacg gggtggggca gttggccgac
      841 ggcacctatg tggtggtcgg caccggtgtg cgcggcgatc tgtggtcgtc gtcgccggtg
      901 atcgtcgccc gctcggccga tggtgtgacg tggagcgagt cgagcttcga cgcgctgctc
      961 accgaggccg atggtgcatc cgcatggtgg gacctccagc gcgtcgtcat cggtcctgag
     1021 gcgatcgctg tcgtcgcggc ggtcaatacc gatccgtnnn nnnnnnnnnn nnccacgacg
     1081 ggatcaccgt gtcgatggtc gatcgcgacg gcacgatcga attccacgac cgctcgggcg
     1141 agttgctcgg cacctatgac ccgcgcaccg cggcgagcgg tggcggtgct gcaggcggca
     1201 acgtccaggt cgacaccgac ggcaccccga tgctgatcga cgacgacggc acggtgatct
     1261 accgcgtgac cgacaaggac tacgagagct tcagcaacat ggtcacgtgg ccgtcggtgg
     1321 agtacagcct cgccgtgctg tactccaccg acggcctggc ctggtcgcgc gacgacgtcg
     1381 acgagatcgg cgatgtcggg tcggtcagcc ccggggggnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncgct
     1501 ccggctgacc cgcaggtcag gacgttggtc gttatcagct cggtgtcgtt gccagatcgg
     1561 cggtctgctg gcacgatggc tgcatgagca cacccagcat caatatcggt atcagcgagt
     1621 ccgatcgcgc cgcgatcgca gagggtctcg gcaagttgct cgccgacacg tacacgctgt
     1681 atctcaccac gcacaacttc cactggaacg tcaccggacc gatgttcaac acgttgcaca
     1741 ccatgttcat gacgcagtac accgagctgt ggaacgcggt cgacccgatc gccgagcgga
     1801 ttcggtcgct cggccacccg gcgcccggtt cctacgctca gttcggttcg ctcagctcga
     1861 tcgacgacgc cccggccgat ccgcccaacg cgatggacat ggtgcgcatc ctggtcaacg
     1921 gccacgagtc cgtcgcccgc accgcgcggg ccgtgttccc cttggccgat gctgccagcg
     1981 acgagcccac cgcagacctg ctcacccagc gcctgaccgt ccacgaacag accgcctgga
     2041 tgctccgctc cctgctcgag tgacccgacg cgccgttcac ccgtcagcct gctggcgggt
     2101 gaaggcacct gacgcgttca tcgcgccggt cgatgcgggc gtgtcgatct tcaccaacat
     2161 cgacaccaac atcgacacca acgtcgtcat cgacgtgagc ggctacatca cgccccagcc
     2221 actccactgt ctgacccgtc agccaacagc gagaaagttg cttgattcgt caactactgg
     2281 caccctgact acatgagcac gaccgttcag tccgcacccc agtcagctgt tggcacacgg
     2341 ttacccgtgt atttcatctc ccacggcgga gggccgtggc cgtggctcac cnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc gctgcaagac atcccggctg ggctcggtgg
     2461 tgtcacgccg cgggcgatcc tgatggtgtc ggggcactgg gaaaccaccg acttcacggt
     2521 gcagacccac cccaacccac cgatgctgta cgactacggc ggcttccccg agttcaccta
     2581 ccacgtgcag taccctgcgc ccggcgcacc cgacgtcgcc gcgcgggccg ccgacctgct
     2641 ccgcgccgcg ggctggccgg tgggcgagga tgccgagcgc ggcttcgacc acggcgtgtt
     2701 cgcgccactc gtcgtcgcct accccgacgc cgacgttccg gtggtgcagc tgtcgatcaa
     2761 gcgcagcttc gacccggccg agcacctcgc cgcgggccgt gccctcgccc cgctgcgcga
     2821 cgagggcgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngt
     3001 cgcccacccc cgcgaagacc acctgatccc gctgatggtc gcggtcggcg cggccgagtc
     3061 cgaacaggct gagcgcacct accacgagac cgccttcatg aactcgatca cctcctccag
     3121 cttccgtctc ggccccaacc ccacataacc cgcaacggaa accctggctc gcggacacac
     3181 actgcaccgc accgcaccgc accgagggtc atctgttcat cnnnnnnnnn nggtgagggc
     3241 ttcgatggtc ttggcgacgc ggcgggcgcg ggtttcgtcg gtcttggcgc tggtgatcgc
     3301 ctccgcgtgc tggcgttgat ggctgtacga caacgccgcc caggctcgcg ccgcctgcgg
     3361 gtcttcggcg agtgccgcgg cgagcggttc gggaacctcg accacgcgag gctgctcgtc
     3421 ggcctccaac gtgacctcga cctcgtcacc agcgccacga ccggtcgcct tccgggtcgc
     3481 ggcgttgaac gagatcaggt actgaccacc catgctggcg atcgtgttgc ggtagctgta
     3541 cgcgccgtcg atcgtgacca cgactggaac ccgcttgcca cggttgaacc cgagcactac
     3601 ctcctccggc acgacgatcg cgacgttgtt ccccccagcc gcccacaacc tggtccgaaa
     3661 cgtagccata cagcaatctc caatcaggtc tgtccgacgg tcgtcgggtg gtgagagttc
     3721 atgggaactt ggtgaggacg cgctggatgg tcagccaggt gcgggtggtg atgttcttgc
     3781 tgcacgcctg ttccagccag gtcatgaagt cgggcgtcgt gccggggatg ctgttgtccg
     3841 tcacggcgag caccgcgcgc gcatccgcgt catagcccac gactcggaac agttcgctgt
     3901 cagacgttgg cagtgggagc ggtggatcgc cgtccttgat gaacgtcgcc gtcagatacg
     3961 tgccgcgctg gtgggtcaat ccggcgaacg gatcgctcgc ccacagtcgc tgcaactgct
     4021 cgcgcgtgcg cacgatcgtc tgtccgggaa tgcccagatc accgttgagc gcggcctgta
     4081 tctgcgcttc cagggccacc gcatcggtct ccgcggtccc gaacacgatg ttcccactgg
     4141 cgagcaccga cgccacgnnn nnnnnnnnnn nnnnnnnnnn nncgttcgaa cacaccgcgc
     4201 agtttcgccc cggtcatctg cgggttgctc ggcatgatcc cccgcagaag cgccgcaaac
     4261 tgccccatgt gtctcaccct aatcaggtgg caaggacgta cgcggcgagg ccgacgactg
     4321 cgaggccgac gatcaggtcg gtcgcggtgg caagggtctc ttgcgcgttc ttgccctgct
     4381 tcggcaggtg caggacgccg gcgatgagca ggaacacgcc cccggcgagt cccgccggca
     4441 gagcccagtc ggagacgagc gcgaagaggc ccgcgagtcc catcccgatg ttggccgtac
     4501 cgagctcacg gatcagttgc acctcgtg
//
LOCUS       DAORNF010000199         4526 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_14247_length_4526_cov_1.985686, whole genome shotgun sequence.
ACCESSION   DAORNF010000199 DAORNF010000000
VERSION     DAORNF010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4526
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14247_length_4526_cov_1.985686"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(43..639)
                     /locus_tag="PLV68_09085"
     CDS             complement(43..639)
                     /locus_tag="PLV68_09085"
                     /inference="COORDINATES: protein motif:HMM:NF036525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRE01442.1"
                     /translation="MSSPTPTARRARGSRRRELLAIAADLFAERGFAGVTVDDIGAAA
                     GISGPALYHHFESKEAILGEMLVAISEQLLAGGRACRAAAPDDRLLADLIDMHVDFAV
                     DQQALITVHFRDLPHASAEDQQRVRRLQRQYAEIWVRALIAVTPGLSVTSARAAVHAV
                     FGLINSTPFSTRPRRPDTRADMVTLLRTMSTGAFTALT"
     gene            862..>1174
                     /locus_tag="PLV68_09090"
     CDS             862..>1174
                     /locus_tag="PLV68_09090"
                     /inference="COORDINATES: protein motif:HMM:NF013225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HRE01443.1"
                     /translation="MARAQLGGPESSRQRHVARGKLLPRDRVTTLLDPGSPFLELSPL
                     AAHGMYGGEVPSAGIICGIGQVSGRLCVVVANDATVKGGTYHPITVKKHLRAQEVALD
                     NH"
     assembly_gap    1175..1407
                     /estimated_length=233
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1408..2375
                     /locus_tag="PLV68_09095"
     CDS             <1408..2375
                     /locus_tag="PLV68_09095"
                     /inference="COORDINATES: protein motif:HMM:NF013225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HRE01444.1"
                     /translation="GPPLVKAATGEVVSAEDLGGGLMHSSISGVTDHLADDDRHALQI
                     VRQIVATLPPPHTAPWQVLASIEPVVDPASLHAVIPADARTPYDVREVIARIVDASQF
                     HEFKALYGDTLVCGFAHVWGHPVGIIANNGVLFSESALKGAHFIELCDRRNVPLVFLQ
                     NITGFMVGRDYEAGGIAKHGAKMVNAVATARVPKLTVIIGGSYGAGNYSMCGRAYSPR
                     FLWMWPNARISVMGGEQAASVLATVRRDQIEGRGDEWSSAEEDAFKQPIRDQYEAQGH
                     PYFSTARLWDDGVIEPGQTRMVLGLALQAAGQAPLGPVNYGVFRM"
     gene            2386..>2867
                     /locus_tag="PLV68_09100"
     CDS             2386..>2867
                     /locus_tag="PLV68_09100"
                     /inference="COORDINATES: protein motif:HMM:NF012510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HRE01445.1"
                     /translation="MFDSVLIANRGEIAVRIIRTLRALGIRSIAVYSDADRDSRHVRD
                     ADDAVHIGPAAAALSYLDVGRVIDAARRSDAQAIHPGYGFLSENVVLAQACADAGIVF
                     IGPPIAAIEAMGDKIRAKQTVAANGVRVVPGISGAGLSTDELVALATRDVGLPALIKP
                     S"
     assembly_gap    2868..2877
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2878..>3336
                     /locus_tag="PLV68_09105"
     CDS             <2878..>3336
                     /locus_tag="PLV68_09105"
                     /inference="COORDINATES: protein motif:HMM:NF014805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01446.1"
                     /translation="GGGKGMRLVTDAAQFADEIAAASREARAAFGDDTLLIERYIERP
                     RHIEVQVLADAHGNVVHLGERECSLQRRHQKVIEEAPSPYLTPDQREALGAQAVAAAR
                     ACGYTNAGTVEFIVSGERADEAYFLEMNTRLQVEHPVTEMVWGLDLVELQL"
     assembly_gap    3337..3488
                     /estimated_length=152
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3489..4451
                     /locus_tag="PLV68_09110"
     CDS             <3489..4451
                     /locus_tag="PLV68_09110"
                     /inference="COORDINATES: protein motif:HMM:NF014804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01447.1"
                     /translation="HVRVDSSLLTGTVVGSDYDPMLAKIIAWGPDRDTARRRADAALA
                     HTAVLGVTTNITLLRQLLTDPQVAAGVVDTGLVERIVAEQPVPSSADVRHDLAAIAAA
                     LVMLDASREPGPWGDRTGWRLGGPGPIEQRIIDADGMSSDGGETVVVLYRAGQDWLAT
                     VDGREHHVHAVHDEQQIVIDRNDNGDSDRDGDGRVRFVIATDNTGSTTTSDGMWLGAG
                     GTAWHFQPAALQRQHRTRGGAAGHAVVSPMPGTVVTVPVAVGDAVTAGQAVAVVEAMK
                     MEHTLRAAADGIVREVTVAAGDRVELRQTLVLLEPVTSTSPEAT"
BASE COUNT          687 a         1426 c         1420 g          598 t
ORIGIN      
        1 caacctcgcc gcatatctcc aacgaatcaa cgactcacga cactaggtga gggcggtgaa
       61 ggcgccggtg gacatggtgc ggagcagggt caccatgtcg gcgcgggtgt cggggcggcg
      121 ggggcgcgtg ctgaaagggg tggagttgat caggccgaac acggcgtgga ccgcggcgcg
      181 agcgctggtg acgctgaggc cgggggtcac cgcgatcagg gcgcggaccc agatctccgc
      241 gtactgacgt tgcaggcgcc ggactcgctg ttggtcctct gcactggcat ggggcaggtc
      301 gcggaagtgg accgtgatca acgcctgttg gtcgacggcg aagtcgacgt gcatgtcgat
      361 gagatccgcc agcaagcggt catcgggtgc ggccgcgcgg caggcccgtc cgccggcgag
      421 caactgctcg ctaatcgcga cgagcatctc accgaggatc gcttccttgc tctcgaagtg
      481 gtggtacagc gcaggaccgg aaatccccgc cgcagcaccg atgtcgtcga ccgtgacccc
      541 cgcgaagccg cgctcggcaa acaggtcggc tgcgatcgcc agcaactccc gtcgcctgct
      601 gcctcgcgcg cgccgagcgg tcggggtggg cgaagacatc gacgcgatgg taatcgtcag
      661 gttgacaagg cggttacctt aacgcagact taggaacctc tcgcaaccgc gcccactcgg
      721 tcgtggcaga tccgaacgac accatgcatc cgctgcaact caccactcat ctcgaccgga
      781 acgatccgtc ggcgcagacc aacaccgacg cgctcgatcg cgtcgtcgcc gatctgcgcg
      841 cgggcggctt ggcgcgccga gatggcacgg gcacaactcg gcggccccga gtcctcccgc
      901 cagcgtcacg tcgcacgcgg caagctgttg ccgcgcgatc gcgtcacgac gttgctcgac
      961 cccggctccc cgttcctgga actgtcaccg ctggcggcgc acggcatgta cggcggcgag
     1021 gtgccgtcgg cggggatcat ctgcggcatc ggccaggtgt cgggccggtt gtgcgtcgtc
     1081 gtcgccaacg acgcgacggt caagggcggc acctaccacc cgatcacggt caagaagcac
     1141 ctccgcgccc aagaggtcgc gctcgacaac caccnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnngcg ggcccccgct ggtgaaggcc gcgaccggcg
     1441 aggtcgtgtc ggcggaggac ctcggcggcg ggttgatgca ctccagcatc tccggcgtca
     1501 ccgatcacct ggccgacgac gaccgccacg ccttgcagat cgtgcgccag atcgtcgcca
     1561 ccctgccccc accccacacc gcgccgtggc aggtgctggc gagcatcgag ccagtggtcg
     1621 accccgccag cctgcacgcg gtgatccctg ctgacgcgcg cacgccgtac gacgtgcgcg
     1681 aagtgatcgc ccgcatcgtc gacgccagcc agttccacga attcaaggcg ctctacggcg
     1741 acacgctggt ctgcggcttc gcccacgtgt ggggtcatcc ggtcggcatc atcgccaaca
     1801 acggcgtgct gttctccgag tcggcgctga agggcgcgca cttcatcgag ttgtgcgacc
     1861 ggcgcaacgt cccgctcgtg ttcctccaga acatcaccgg gttcatggtc gggcgcgact
     1921 acgaggcggg cggcatcgcc aagcacgggg cgaagatggt caacgcggtc gccaccgcgc
     1981 gggtgccgaa gctgacggtg atcatcggcg gttcctacgg ggcgggcaac tacagcatgt
     2041 gcggtcgggc ctactcccct cgctttctgt ggatgtggcc caacgcacgc atctcggtga
     2101 tgggcggcga gcaggctgca tcggtgctcg ccaccgtgcg ccgcgaccag atcgaagggc
     2161 gcggcgacga gtggtcgagc gcggaggagg acgcgttcaa gcaaccgatc cgcgaccagt
     2221 acgaagcgca gggacacccc tacttctcca ccgcgaggct gtgggacgac ggcgtgatcg
     2281 agcctggcca gacccggatg gtgctcggcc tcgcgttgca ggccgccggc caggcaccgc
     2341 tcgggccggt caactacggc gtgttcagga tgtgaggtga cgcgcgtgtt cgacagtgtg
     2401 ctcatcgcca accgcggcga aatcgccgtg cggatcatcc gcaccctgcg tgccctcggc
     2461 atccgctcga tcgccgtcta cagcgacgcc gatcgcgact cgcgccatgt gcgtgacgcc
     2521 gacgacgcgg tgcacatcgg ccccgctgcc gcggccctca gctacctcga cgtcggtcgc
     2581 gtgatcgacg cggcgcgacg cagcgacgcg caggcgatcc accctggcta cgggtttctg
     2641 tccgagaacg tcgtgttggc ccaagcgtgt gccgacgcgg gcatcgtctt catcgggcca
     2701 ccgatcgcgg cgatcgaagc gatgggcgac aagattcgcg ccaagcagac cgtcgccgcc
     2761 aacggcgtgc gcgtcgtacc aggcatctcg ggtgccgggt tgagcaccga cgaactggtc
     2821 gcgctggcga cgcgcgacgt cgggttgccc gcgctgatca agccatcnnn nnnnnnnggg
     2881 ggcggcaagg gcatgcggct ggtgaccgac gcggcccagt tcgccgacga gatcgccgcg
     2941 gccagccgcg aagcccgcgc cgcattcggt gacgacaccc tgctgatcga gcgctacatc
     3001 gagcggccac gtcacatcga agtgcaggtg ctggccgacg cccacggcaa cgtggtgcac
     3061 ctcggtgagc gcgagtgcag cttgcagcgc cgccaccaga aggtgatcga agaggcaccc
     3121 tcgccgtacc tcacgcccga ccaacgcgag gcactcggcg cccaggcggt cgccgcggcg
     3181 cgcgcctgcg ggtacaccaa cgctggcacg gtcgagttca tcgtctccgg cgagcgcgcc
     3241 gacgaggcgt acttcctgga gatgaacacg cgcctgcagg tcgaacatcc ggtgaccgag
     3301 atggtgtggg ggctcgacct ggtcgagctc cagttgnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnca cgtgcgggtc gactcgtcgc tgctgaccgg aaccgtcgtc ggcagcgact
     3541 acgacccgat gctggccaag atcatcgcct ggggacccga ccgcgacacg gcgcggcgac
     3601 gtgcggatgc cgcgctggcg cacaccgcgg tgctcggtgt gaccaccaac atcacgctgc
     3661 tgcgccaatt gctgacggat ccgcaggtcg ccgccggcgt ggtcgacacc ggcctggtcg
     3721 aacggatcgt cgccgaacaa ccagtgccgt cgagtgctga cgtcaggcac gatctggccg
     3781 cgatcgcggc cgcactggtc atgctcgacg catcccgcga gcccggcccg tggggcgacc
     3841 gtaccggctg gcggctcggt ggtcctgggc cgatcgaaca acggatcatc gacgccgacg
     3901 gcatgtcgtc cgacgggggc gagacggtgg tcgtgctgta ccgcgcgggg caggactggt
     3961 tggcgacggt cgacggacga gagcaccacg tccacgcggt acacgacgag cagcagatcg
     4021 tgatcgaccg caacgacaac ggcgacagcg atcgcgatgg cgatgggcgg gttcggttcg
     4081 tcatcgccac cgacaacacc ggtagcacca ccaccagcga cggcatgtgg ctgggcgcgg
     4141 gcgggacggc gtggcacttc caacctgcag cactgcagcg tcagcaccgc acccgtggag
     4201 gcgccgcggg tcacgccgtc gtcagcccca tgcccggcac ggtcgtgacc gtgcccgtcg
     4261 cggtgggcga cgcggtgacc gcaggccaag cggtcgccgt ggtcgaagca atgaagatgg
     4321 aacacaccct gcgtgccgcg gccgacggca tcgtgcgcga ggtcaccgtc gccgctggcg
     4381 atcgggtcga gttgcgccaa accctcgtcc tgctcgaacc cgtcaccagc acgtcaccgg
     4441 aggccacctg atgccgatcg acctcacacc tgaacacgac gcgttccgcg acatggtccg
     4501 atccttcgcg gaggcggaga tcgcgc
//
LOCUS       DAORNF010000200         4516 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_14284_length_4516_cov_1.674288, whole genome shotgun sequence.
ACCESSION   DAORNF010000200 DAORNF010000000
VERSION     DAORNF010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4516
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14284_length_4516_cov_1.674288"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>263)
                     /locus_tag="PLV68_09115"
     CDS             complement(<1..>263)
                     /locus_tag="PLV68_09115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01448.1"
                     /translation="RRDELNETKPHRPGLLDGGPLRELLELRHEITSAIEHAERDLEH
                     GPRERRDVEAKKAASEKQLAATVAALERAHVVGASLGGMIAQA"
     assembly_gap    264..466
                     /estimated_length=203
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<467..1594)
                     /locus_tag="PLV68_09120"
     CDS             complement(<467..1594)
                     /locus_tag="PLV68_09120"
                     /inference="COORDINATES: protein motif:HMM:NF024991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRE01449.1"
                     /translation="MRRDGRPVSESVLDRALTTQAIIDQEDGLIAWADRRLAYDGVDE
                     PAVASRSGIAMNVAQAAAAAAVAGYDDLVLVVGPAGTGKTTALAPAVEQLRGDGRAVF
                     GVAPSATAAVVETPSAAEVLHLETGVVADTLDKLLIEHRLNRPPDHRYDLPVGATVIV
                     DEAGMMPTAKLADLADLADIKGWRVALVGDPLQFSAVGRGGMFGLFVNTFGAIELDRV
                     HRFDNEWERDASLRLRRGDVDVADDYDAHGRLYGGTQTQMERACVARWWELREQGRSQ
                     LLMAPTNDATERLNERCQLTRIRAGEIDPNGPTVKAGPYTLHVGDEIATRHNDRRLVT
                     DSGDMVRNRATWTIDTVHPDRSLTVTGRNGTVRLPAGYVAAH"
     assembly_gap    1647..1715
                     /estimated_length=69
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1716..2787)
                     /gene="mobF"
                     /locus_tag="PLV68_09125"
     CDS             complement(<1716..2787)
                     /gene="mobF"
                     /locus_tag="PLV68_09125"
                     /inference="COORDINATES: protein motif:HMM:NF041492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobF family relaxase"
                     /protein_id="HRE01450.1"
                     /translation="MTVRVTTLKGADAGAYYVEALPNYYLQSGEPRGLWFGDGAQLLG
                     LGGEVDGDAFIALMGGMDPSRPDRHLGRRYDDKSVRGFDVTCSAPKSVSVLFAVGDLD
                     TRREVLGAHDAAVTALAGWIECHAHTRYRIGGEVSIVDAEGIVAAAFRQHTSRALDPQ
                     LHTHLVIANRVKSPDGRWLALDGRLIKHDQQSLSAIYHAGLRAELTRRLGVRWHAPEH
                     GIAEIRDVADDVLVEFSSRTADVRRRIDDKLDRFAETMGREPTPRERWKLEREAAIDS
                     RPAKAHSLDADVLHAQWVEQVRVLGYEPSRIVTDAVEQVAVRHGIDSATAASIVDRAM
                     AAMSERQSSWRPTELHRELGAAI"
     gene            complement(2877..3227)
                     /locus_tag="PLV68_09130"
     CDS             complement(2877..3227)
                     /locus_tag="PLV68_09130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRE01451.1"
                     /translation="MNESRDDAASTAPDFLTVEEAAEVLRLGRTAAYKEVRKYEASHG
                     ATGIPVERFGKQFRVPRCKIEERLGGPITWPLRSQQPAVETPVASATTPTTRSRATRR
                     RSPRDEQTPRLFPL"
     gene            3553..>4516
                     /locus_tag="PLV68_09135"
     CDS             3553..>4516
                     /locus_tag="PLV68_09135"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HRE01452.1"
                     /translation="MRGHVARKGNRYYAVVYEGFDPATGQERHRWHAAGPTRKGAEKV
                     LGELIKRMHDGDYRAPDRITLGDYLLERWLPTKQAQIRPSTFSSYRNNVITHVVPRIG
                     SIPLQKLQPEDLDTFYAHLLRNGRRNGAGGGLAPKTVRIIHGIIRKALADALRKGSVT
                     RNVADLADPPKVRLGGSRTMTVWSADELRDFLASIEDSEWYVPIFLAANTGMRRGEVL
                     GLTWRNVDLDGARLVVSQQILSVEYAATVADVKTAHSRRTIDLDARTVAVLRTWRRHQ
                     LEQKMSTGRRDDDEFVFTHPDGGPIHPDFFSQSWQRLMRNSEHRR"
BASE COUNT          679 a         1426 c         1432 g          707 t
ORIGIN      
        1 cgcctgggcg atcatcccgc cgagcgatgc ccccacgacg tgcgcccgtt cgagagctgc
       61 cactgtggcg gcgagttgct tctcgcttgc ggccttcttg gcttcgacgt cgcgccgttc
      121 acgtggcccg tgctcgagat cgcgttcggc gtgctcgata gccgaggtga tctcgtggcg
      181 tagttcgagt agttctcgta gcggtccgcc gtcgaggagg ccgggtcggt ggggtttggt
      241 ctcgttgagt tcgtcgcggc gggnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngtgt gcggccacgt
      481 agccggcggg caggcgaacg gtgccgttgc ggccggtgac ggtcagcgac cggtcggggt
      541 ggacggtgtc gatggtccag gtggcgcggt tgcggaccat gtcgccgctg tcggtcacga
      601 gccggcggtc gttgtggcgg gtggcgatct cgtcgccgac atgcagcgtg tacgggccag
      661 ccttcaccgt tggcccgttg gggtcgatct cgccggcgcg gatgcgggtc agctggcagc
      721 gttcgttgag acgttcggtg gcgtcgttgg tcggcgccat cagcaactgc gacctgccct
      781 gctctcgtag ctcccaccag cgggcgacgc acgctcgttc catctgggtc tgcgtcccgc
      841 cgtagagacg gccgtgggcg tcatagtcgt cggcgacgtc gacgtcacca cggcgcagcc
      901 ggagactggc gtcgcgttcc cattcgttgt cgaagcggtg gacgcggtcg agttcgatcg
      961 cgccgaaggt gttcacgaag agtccgaaca tgccaccgcg gccgaccgct gagaactgca
     1021 aagggtcacc gacgagagcg acacgccatc ccttgatgtc ggcgaggtcc gccaggtcgg
     1081 cgagcttggc ggtgggcatc atcccggcct cgtcgacgat cacggtcgct ccgaccggaa
     1141 ggtcgtagcg gtgatcgggt ggccggttga gccggtgctc gatgagcagc ttgtcaagcg
     1201 tgtcggcgac gacaccggtc tccaggtgga gcacctcggc cgccgacggc gtctcgacga
     1261 cggccgcggt cgctgacggc gcgacaccga acaccgcccg cccgtcgcca cggagctgct
     1321 cgaccgcagg ggcgagcgcc gtcgtcttgc cggtacctgc cgggccgaca acgagcacga
     1381 ggtcgtcata gccagcgacc gccgcggcgg ctgcagcctg ggcgacattc atcgcgatcc
     1441 cactgcgcga cgcgaccgcc ggttcgtcga caccgtcgta ggcgaggcgc cggtctgccc
     1501 acgcgatcag gccgtcttcc tggtcgatga tcgcctgggt ggtcaacgcc cggtcgagca
     1561 ccgactcgga caccgggcga ccgtcacggc gcaacagcgc atccgatggg accggccgcg
     1621 agatgtcgat gcaccggctt acagtgnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnggatg gcggcaccca gctctcggtg
     1741 caactcggtc ggtcgccacg acgactgcct ctcgctcatc gcagccatcg cccgatccac
     1801 gatcgacgca gcagtcgcac tatcaatgcc gtgcctgacg gcgacctgtt cgacggcgtc
     1861 ggtgacgatt cgtgacggtt cgtacccgag cacgcggacc tgctcgaccc attgagcgtg
     1921 caagacatca gcgtcgagcg agtgggcttt ggccgggcgg ctgtcgatcg cagcctcgcg
     1981 ctccagcttc caacgttccc gcggtgtggg ctcgcgaccc atcgtctctg cgaagcggtc
     2041 gagcttgtcg tcaatgcgtc ggcggacatc ggcagtgcgg ctggagaact ccaccaggac
     2101 gtcatcggca acgtcacgga tctccgcgat cccatgctcc ggggcatgcc agcgcacacc
     2161 cagccggcgg gtcagctccg cgcgcaagcc ggcgtggtag atcgccgaca gggattgctg
     2221 atcgtgcttg atcaaccgcc cgtccaacgc cagccaacgc ccatcaggcg acttcaccct
     2281 gttcgcgatc accagatggg tgtgcaactg ggggtcgagc gcccggctcg tgtgctggcg
     2341 gaacgcagcc gccacgatcc cttcagcgtc gacgatcgac acctcgccac cgatccggta
     2401 gcgggtgtga gcgtgacatt cgatccagcc ggccaacgcc gtcaccgccg cgtcgtgggc
     2461 gccgagcacc tcgcggcggg tgtcaaggtc gccgactgcg aacagcaccg acaccgactt
     2521 cggtgcggag catgtcacgt cgaacccgcg caccgacttg tcgtcgtacc ggcggccgag
     2581 atgacggtcc gggcgtgacg ggtccatccc gcccatcagc gcgatgaagg catcgccgtc
     2641 gacctcaccg cccagcccga gcagctgcgc gccgtcaccg aaccacaggc cacgcggttc
     2701 accggactgc aagtagtagt tcggcagggc ctcgacgtag tacgcgcctg cgtcggctcc
     2761 cttcaaggtc gtcactcgca cggtcaccac acacccccgt ccagtcgcgt ggctgtgcac
     2821 tcgtctatcc atgaagtgac gtgaggttgg gtgggctgga gttgttgcat ggcgacctac
     2881 agggggaaca gtcgcggcgt ctgctcgtca cgcggtgagc gtcggcgagt ggcacgcgag
     2941 cgggtggtgg gagttgtcgc ggacgcgact ggtgtctcaa cggccggctg ctgagagcgg
     3001 aggggccagg tgatcggacc gccgaggcgc tcttcgatct tgcagcgagg gacccggaac
     3061 tgcttgccga accgctcgac cgggatgccc gtcgcgccgt ggctcgcttc gtacttgcgc
     3121 acctctttgt aggcggctgt gcgaccgagg cgcagcacct cggcggcctc ctcgacggtg
     3181 aggaagtcgg gcgctgtgct tgcggcatcg tctcggctct cgttcatctc gctcatggag
     3241 tcatgcctct cgcgaacggc tgtcgtcact gcttcggtgt tgaacaacgg acatcgagga
     3301 ggcacatcgg ttgaattcgc gcgtgggtcc tcagtgggtt acagaggtcg attcgcccta
     3361 cccgcagcac gaatcgccca gaaatctcag ctcttgcgca ggggcgtaca atccggtcat
     3421 ggcgcgcgcc tcgtcgcccg tgagcgcctg ggagacccgc ctgtctggcg gtgacccggt
     3481 ggccgagagc cgccgagcgt gcgagttccg cagggcgcac caaacgtcac caacgatcac
     3541 gaggtgtatg ccatgagggg acacgtggcc cgcaagggca accgctacta cgccgtcgtg
     3601 tacgagggct tcgacccggc gaccggacag gaacgccatc gttggcacgc cgcaggaccg
     3661 acacgcaagg gagccgagaa ggtgctcggc gagctcatca aacggatgca cgacggcgac
     3721 taccgcgcac ccgaccggat cacgctcggc gattacttgc tcgagcgctg gcttccgacc
     3781 aagcaggccc agattcggcc gtcgacgttc agctcctacc gcaacaacgt gatcacccac
     3841 gtcgtgccgc gcatcggttc gatcccgctg cagaagctgc agcccgagga cctcgacacg
     3901 ttctacgcgc acttattgcg caatggccgg cgcaatggtg ccggcggcgg gctcgcgccg
     3961 aagacggtgc ggatcatcca cggcatcatc cgcaaggcac tggccgacgc gctccgcaag
     4021 ggcagcgtca cccgcaacgt cgccgatctt gccgacccac cgaaggtccg cctcggcggt
     4081 tcgaggacga tgactgtgtg gtcggccgac gagctgcgcg acttcctcgc gagcatcgag
     4141 gacagcgagt ggtacgtgcc gatcttcctc gccgccaaca ccgggatgcg ccgcggtgag
     4201 gtgcttggcc tcacatggcg caacgtcgat ctcgacggag caaggctggt ggtctcgcaa
     4261 cagatcctgt cggtcgagta cgccgcgact gtcgccgacg tcaagaccgc gcacagccgg
     4321 cgcaccatcg acctcgacgc gcgcaccgtc gccgtgctga ggacgtggcg ccgtcaccaa
     4381 ctcgagcaaa agatgagcac cggccggcga gacgacgacg agttcgtgtt cacccacccc
     4441 gatggcggac caatccaccc cgacttcttc tcgcagtcat ggcaacggct gatgcgcaac
     4501 agcgagcatc gccgca
//
LOCUS       DAORNF010000201         4492 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_14382_length_4492_cov_1.671625, whole genome shotgun sequence.
ACCESSION   DAORNF010000201 DAORNF010000000
VERSION     DAORNF010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4492
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14382_length_4492_cov_1.671625"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..885)
                     /locus_tag="PLV68_09140"
     CDS             complement(<1..885)
                     /locus_tag="PLV68_09140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01453.1"
                     /translation="MEGEFVRRPQAEQRPLVAPPLVASQRGPVAAVQRVMRVGAADDR
                     AEREADVIADEIVGRLHDGTAVVTPSGALLQSGDDDGAGRIRRSAMSPIEVAAVQPAA
                     RIQRLTGSAHQPAEAQHALVQRAAITTFGGTFDTAQYEAVSAKPAAGKQTVGGVIDLA
                     FTPNDLVDGKVGLVQTGKAKRGGAVDIGPNDHDKRARAQKAGGDEGRFLDRSNYKDNP
                     IFGMEASKTDLAPIDEGVGISMTTDITGKPSEGRNLVAKDNKDATVTKADPAALVDPA
                     RTSARAGVIGANNAVDGAT"
     gene            complement(933..>1166)
                     /locus_tag="PLV68_09145"
     CDS             complement(933..>1166)
                     /locus_tag="PLV68_09145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01454.1"
                     /translation="SGVSAVAAEVTSTDARTSGFITVHPCQAKVPGVSMVRNIGTNVG
                     ATTVTGILDPQGRWCLAPNVAMDIVVDVYGYYD"
     assembly_gap    1167..1188
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1189..2255)
                     /locus_tag="PLV68_09150"
     CDS             complement(<1189..2255)
                     /locus_tag="PLV68_09150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01455.1"
                     /translation="MLQREGGGEWGGWVLKVRVEGTAGSVEVSGWDLRSKLGLKSSWF
                     AVRGSADNSTGGSGGGGSGGGSGSDPRCGLRVPPPVTSSGVDDRASLFTPITPRRLID
                     TRIGFGTAQAPIGVGCTVQIDPGVPDDTTAVVINVTTVDPDLNGYLTAYPCGTAMPLA
                     SIVPALTGRIVPGSAIVPVGADGTVCIYSMRATELVVDLTGYYRASEGVGFEPLVTER
                     RFDSRNGARLANGATVRVQVGGIGNVPGHGARQRCEHQRVHHGLAVQRRTPDHLGAQH
                     RTWSGGGQPRASRSRRLRRGVHVRAACDAPGPRRQRLVRAYRRRQLSRPEPGPCARFA
                     RGLRRDRSLLGRPEPRPHHGG"
     gene            complement(2469..>2868)
                     /locus_tag="PLV68_09155"
     CDS             complement(2469..>2868)
                     /locus_tag="PLV68_09155"
                     /inference="COORDINATES: protein motif:HMM:NF020075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HRE01456.1"
                     /translation="PSDLIGACEPSTGRVRYYRGAIRTVNDAAGANRTVSELPVEQYV
                     RSVVANELHGSVGALGGGRGAQALQAQAVAGRTYALIENQYPYAKTCDNNCQSYLGAG
                     YRVGVGGGWSSAEHPAADAAVLATAGVVRR"
     assembly_gap    2869..2878
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2879..3428)
                     /locus_tag="PLV68_09160"
     CDS             complement(<2879..3428)
                     /locus_tag="PLV68_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01457.1"
                     /translation="MGSLGIAISEAVAMAVAVTGVTATTVTPPVVAAPATPGVTGVMT
                     SVVFDGHGYGHGVGMSQWGAIGYAVDQGWSAAQILGHYYGGTVASTAPDDLITVRLGK
                     FDGAVQTALVHDLGALTVDGLAGGPWRSVAIRETAPNRYSVYARADTSVCPASADDLV
                     AAGWTVVGADLTVVTARPATDTS"
     gene            3594..>3817
                     /locus_tag="PLV68_09165"
     CDS             3594..>3817
                     /locus_tag="PLV68_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01458.1"
                     /translation="MAAPTNRRSPLQKLALPEGTLPVGAGLIVAGISSYLFFKIGDKA
                     LGKDGFKPINSLWFATFFLAPGFFLPLEQE"
     assembly_gap    3818..3860
                     /estimated_length=43
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3874..>4122
                     /locus_tag="PLV68_09170"
     CDS             3874..>4122
                     /locus_tag="PLV68_09170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01459.1"
                     /translation="MVPLAAAMAAIVVVVVLIASGKITHSFFEDDWVVTFALIVGFLS
                     YAPVHLARGICSGSNRFVSYGFVMGADGFARIVGCLALW"
     assembly_gap    4123..4278
                     /estimated_length=156
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4279..>4492
                     /locus_tag="PLV68_09175"
     CDS             <4279..>4492
                     /locus_tag="PLV68_09175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01460.1"
                     /translation="AGLVNAGPIAVDLLKGNAQNEAVTTFGYGVLLGRVPLFLFQAVQ
                     AALLPRLAGLAVRNELDEFRRGFRKLL"
BASE COUNT          623 a         1495 c         1452 g          691 t
ORIGIN      
        1 cgtggcaccg tcgacggcgt tgttggctcc gatcacaccg gcgcgtgccg acgtgcgggc
       61 ggggtcgacg agcgcggccg gatcggcctt ggtgacggtt gcgtccttgt tgtccttggc
      121 gacgaggttg cgaccttcgg acggcttgcc ggtgatgtcg gtggtcatcg agatgccgac
      181 gccttcgtcg atcggtgcca ggtcggtctt ggacgcttcc atgccgaaga tcggattgtc
      241 cttgtagttg gaacgatcga ggaaacgccc ctcgtcgcca ccggccttct gggcccgcgc
      301 ccgcttgtcg tgatcgttgg gaccgatgtc gacagcacca ccccgcttcg ccttgccggt
      361 ctgcacgagg cccaccttgc cgtcgaccag gtcgttgggc gtgaaggcca gatcgatgac
      421 gccgccgacc gtctgcttgc cggccgctgg cttggccgag accgcctcgt actgcgccgt
      481 gtcgaacgtg cccccgaagg tggtgatcgc cgcccgctgc accagtgcat gctgcgcctc
      541 agccggctgg tgggcgctgc cggtcaaccg ttggatgcgc gccgcgggct gcaccgccgc
      601 gacctcgatc ggcgacatgg ccgagcggcg gatgcgaccg gctccgtcat cgtcgccaga
      661 ctgcagcagc gcaccggatg gcgtgacgac ggcggtccca tcgtggagtc gccccacgat
      721 ctcgtcagcg atcacgtcgg cctcacgctc ggcgcgatca tcggcggcgc cgacgcgcat
      781 cacccgctgg acggcagcga ccgggccgcg ctgtgatgcg acgagtggtg gtgcgacgag
      841 cggtcgctgc tcggcctgcg gccgccgtac gaactcaccc tccatcgcag ctcctatctc
      901 gtcaccgtcg ccggtcgggt ccccacatca tgtcagtcgt agtaaccgta gacgtccacg
      961 acgatgtcca tcgcgacgtt gggggcaagg caccatcggc cttgggggtc gaggatgccg
     1021 gtgaccgtgg tggcaccgac gttggtgccg atgttgcgca ccatcgacac gccgggcacc
     1081 ttggcctggc acgggtggac ggtgatgaag ccggacgtcc gggcatcggt cgaggtgacc
     1141 tcggccgcga cggccgacac gccgctnnnn nnnnnnnnnn nnnnnnnnca cccgccatgg
     1201 tgagggcgcg gttctggccg gccgagaagc gaccggtcac gccggagccc tcgcgcgaat
     1261 cgagcacacg ggccgggttc agggcgtgat agctgccgtc gccggtaggc ccgaaccagc
     1321 cgttgacgtc gaggaccagg tgcatcgcat gctgcgcgta catgcacacc ccgccgtagc
     1381 cgtcgagacc gacttgcacg tggttggcca ccgccactcc aggtccggtg ttgagcaccg
     1441 aggtggtcgg gcgttcggcg ttgcacggcc agaccgtgat gaacccgttg atgctcgcat
     1501 cgctggcgtg caccgtggcc gggcacgttg ccgataccgc cgacctggac ccggaccgtg
     1561 gctccgttgg ccagccgggc gccgttgcgg gagtcgaacc ggcgctcggt gacgagcggt
     1621 tcgaacccga cgccttcgct ggcgcggtag tagccggtga gatcgacgac cagttcggtc
     1681 gcccgcatcg agtagatgca caccgtgccg tcggcaccga ccggcacgat cgccgagccg
     1741 ggcacgatcc gtccggtcag cgccggcacg atcgaggcca acggcatcgc cgtgccacac
     1801 ggataggcgg tgaggtaccc attgaggtcg ggatcgacgg tggtgacgtt gatgaccacc
     1861 gcggtcgtgt cgtcgggaac acccggatcg atctgcacgg tgcacccgac accgatcggc
     1921 gcctgcgccg tgccaaaacc gatgcgggtg tcgatcaacc gccgcggggt gatcggcgtg
     1981 aacaagctgg cccggtcgtc gacgccggac gacgtgaccg gcggaggcac ccgcaatccg
     2041 caccggggat cgctaccgga gccgccacca ctcccaccgc caccactccc accagtgctg
     2101 ttgtccgcac tgccgcgcac cgcgaaccaa ctcgacttga gccccagctt ggagcgcagg
     2161 tcccagccgc tgacctcgac cgagccggcc gtgccttcga cgcgcacctt cagcacccag
     2221 ccaccccact cgccaccgcc ctcgcgctgc agcaccgtga tgccggtgag cgagccgatc
     2281 gtggaatacg acgactcgac cttggacgcc gcgaccgtgg tggtccagga gtggttgggg
     2341 ttgcccgagt aggagtcgcc cagatcgggt accgccggga acttggcatc gccgccgaag
     2401 gtgtagcgcg ccgtgtatcc acccgacgac gcggcgtaga gcgtgtaggc gagcgccccg
     2461 ttggccggct agcgccgcac gaccccggcc gtggcaagca ctgccgcgtc ggctgcggga
     2521 tgctcggccg agctccagcc gccacccaca ccgacccgat acccggcacc gaggtaggac
     2581 tggcagttgt tgtcgcaggt cttggcgtac ggatactggt tctcgatcag ggcataggta
     2641 cgtcccgcga ccgcctgggc ttgcagcgcc tgggcaccac ggccgccgcc cagcgcgccg
     2701 acgctgccgt gcagttcgtt ggcgaccacc gagcggacgt actgctcgac cggcagttcg
     2761 ctgacggtgc ggttggcccc cgccgcgtcg ttgaccgtgc ggatcgcccc gcggtagtag
     2821 cgcacccggc cggtcgaggg ctcacaggca ccgatcagat cggacgggnn nnnnnnnncg
     2881 ctggtgtcgg ttgctggacg agccgtgacc acggtgaggt cggcgccgac cacggtccac
     2941 ccggccgcaa ccagatcgtc ggccgaggct ggacagaccg acgtatcggc gcgggcgtac
     3001 accgagtacc gattcggagc cgtctcgcgg atcgccaccg aacgccacgg gccaccggcg
     3061 aggccatcga cggtgagcgc accgaggtca tggacgagcg ccgtctgcac cgcgccgtcg
     3121 aacttgccga ggcgcaccgt gatcaggtca tcgggagccg tcgatgcgac cgtgcccccg
     3181 tagtagtggc cgaggatctg cgccgctgac cagccctggt cgacggcata gccgatcgct
     3241 ccccattggc tcatcccgac gccgtgcccg tacccgtggc catcgaacac gacgctggtc
     3301 atcaccccgg tgacaccggg tgtggctggc gccgcaacga ccggcggcgt gacggtcgtc
     3361 gcggtcactc ccgtgacggc caccgccatg gcgacggcct cggagatggc gatgcccagg
     3421 ctgcccatga agacgcgccg acggcgggcc ctcgccgcgt gcctgatccg cgctctgatc
     3481 gtgtggttcg tacgcaccca gggtgcaggt ctagccgcgg cccgtggctg atgcaacgcg
     3541 ctcgcagcac gggaccgggt ggcggcaagc cctgccagat agccttcgcg cccgtggcag
     3601 ccccaaccaa ccggcgttct ccgctccaga agctggcgct gccggaaggc accctgccgg
     3661 tcggcgccgg gctgatcgtg gcaggcatca gcagctacct gttcttcaag atcggcgaca
     3721 aggcgctcgg caaagacggc ttcaagccga tcaacagcct gtggttcgcc acgttcttcc
     3781 tcgcccccgg cttcttcctg ccgctcgaac aagagttnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn gctggtgcgg cgtatggtgc cgctcgccgc ggcgatggcc
     3901 gcgatcgtcg tcgtcgtcgt cctgatcgcc tcgggcaaga tcacccacag cttcttcgaa
     3961 gacgactggg tggtcacgtt cgcgctgatc gtcgggttct tgtcgtacgc cccggtccat
     4021 ctcgcccgcg gcatctgctc gggctcgaat cggttcgtct cctacggctt cgtgatgggt
     4081 gccgacggct tcgcgcgcat cgtcggttgt ctcgcgctgt ggnnnnnnnn nnnnnnnnnn
     4141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4261 nnnnnnnnnn nnnnnnnngc gggcctcgtc aatgccgggc cgatcgcggt cgacctgctc
     4321 aagggcaacg cccagaacga agccgtgacc accttcggct acggcgtgct cctgggccgg
     4381 gtgccgctgt tcttgttcca agcggtgcag gccgcgctgc tgccccgcct ggccggtctc
     4441 gccgtacgca acgagctcga cgagttccgt cgcgggttcc gcaagctgtt ga
//
LOCUS       DAORNF010000202         4487 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_14407_length_4487_cov_1.718412, whole genome shotgun sequence.
ACCESSION   DAORNF010000202 DAORNF010000000
VERSION     DAORNF010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4487
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14407_length_4487_cov_1.718412"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="PLV68_09180"
     CDS             complement(<1..644)
                     /locus_tag="PLV68_09180"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HRE01461.1"
                     /translation="MIRTRVTELLGVRHPVLLAPMGDTAGGRLAAAVSAGGGFGLIGG
                     GYGDVAWLEREIDAAAGARVGIGFITFALDQHDGALDVALAARPVAVQLSFGDPRPYA
                     ERVKAAGALLICQVQSRTEIDHAVEAGADVLIAQGSDAGGHGRGDRATMGLIPSVLDR
                     VGDTPVVAAGGIADGRGLAGALALGAAGVCMGTRFLASAEAISTPAEAEALVAHG"
     gene            823..>1088
                     /locus_tag="PLV68_09185"
     CDS             823..>1088
                     /locus_tag="PLV68_09185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HRE01462.1"
                     /translation="MRITRKADYAVRAMVALARANARYDGVVIPRDLIATQETIPPAY
                     LDDILRKLRNGGLVRSCRGVDGGWSINLDPAEITVGDVLRVMEGP"
     assembly_gap    1089..1100
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1101..1292
                     /locus_tag="PLV68_09190"
     CDS             <1101..1292
                     /locus_tag="PLV68_09190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01463.1"
                     /translation="GGLRPEELADQGTDESMVELWSAMRSSLRSVLDGVTIADLAAGL
                     LPAAISDLASRSAGSARTI"
     gene            1383..2693
                     /locus_tag="PLV68_09195"
     CDS             1383..2693
                     /locus_tag="PLV68_09195"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HRE01464.1"
                     /translation="MLGLNLVFPPRFSDFDHASGAEFDMTTSVWRRAASLAAATISLV
                     GLAACGDDDAPASADSTSQAPVIRVGATLSLTGDFAPDSKLVRDGYQFWYEQVQAAGG
                     ITVGDTTYQLELITQDDGSNREQGVQNVEQFITIDEVDFVLAPWGSGNTAAVAPVTER
                     YEKVTIAPLAMADSLWEQGHGHLYGVLPLASDGMRPVVRLAESLGATSVAVVGTDDLN
                     NTLSAQGAVDEATALGLDVRLHETYPEGQIDLAALISKLKNADADLVIAVAHTSDAEL
                     LLRQFEEQRYFPRALSLTGAVIVPEFLESTEYVFGQTFWSPDLPVQDPLFGTAGEFAT
                     AFEERFGYRPTHDSVASATAGYLLQYAIEQAGTLDSAAVNAVLQTLGIETVLGPVSFT
                     ADTHTNVGGGSYVVQIQDGVPTIVYPADLSRAAPIYPAPGWDQR"
     assembly_gap    2813..2920
                     /estimated_length=108
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2921..3569
                     /locus_tag="PLV68_09200"
     CDS             <2921..3569
                     /locus_tag="PLV68_09200"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRE01465.1"
                     /translation="GYGLQRVLVNRVLVRNVSMTMVMTLGVSFVISTVLAFVWGPDRR
                     SITVGYAASGFDLFGARVPVTRLAIFVLSVALTCGLHAYLTRAEGGRRIQAVSQDPIA
                     AESIGLSVAHYYGLSHALAAAIALAVGGVVPLTQPVHPFISVDLLLIAFAVVVLGGFG
                     SIVAILPAGIVLALGENLEGYLIGLKYQRLAIFAVYLAIATLRPQGLFGRRLYAR"
     gene            3607..>3832
                     /locus_tag="PLV68_09205"
     CDS             3607..>3832
                     /locus_tag="PLV68_09205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01466.1"
                     /translation="MHRVPRGGALVIAVAAVFAAPTVLPDNYWLRFATSTLMLAALAQ
                     SLNITYAMSGYVPLGHLVFFGSGADGAAIIT"
     assembly_gap    3833..3883
                     /estimated_length=51
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3884..>4238
                     /locus_tag="PLV68_09210"
     CDS             <3884..>4238
                     /locus_tag="PLV68_09210"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01467.1"
                     /translation="IAAITSRALLGLRSAYFLMATVALNGLVGEATVVFDGLTGGPHG
                     IIVAPWVRGTPTHVYLVFYYAMAAAVVVIGTVSIIIRRSRLGVGLMAIKGNEDAADAF
                     GVPTQRYKTAVWITTA"
     assembly_gap    4239..4256
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4257..>4487
                     /locus_tag="PLV68_09215"
     CDS             <4257..>4487
                     /locus_tag="PLV68_09215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01468.1"
                     /translation="AVGGVYAHWIGYIDASTSFDLVFSIEVFLVALIGGRWFVVGPLI
                     GALVFRYVEDVAVRNTVEAQFGVLGAVLIVLVL"
BASE COUNT          619 a         1514 c         1435 g          730 t
ORIGIN      
        1 ccgtgggcga ccaacgcctc ggcctcggcg ggggtcgaga tcgcctccgc gctcgccagg
       61 aatcgggtgc ccatgcacac gcccgcggcg ccgagtgcga gcgcacccgc gagaccccgc
      121 ccgtcggcga tgccacccgc ggcgacaaca ggagtgtcgc cgacccgatc gagcaccgac
      181 gggatcagcc ccatcgtggc ccggtcgccg cggccgtgac cgccggcgtc gctgccctgc
      241 gcgatcagca cgtcggcccc cgcctcgacc gcgtggtcga tctcggtgcg cgactggacc
      301 tgacagatca acagcgcacc agccgccttc acccgctcgg cgtagggacg tggatcgccg
      361 aagctgagtt gcaccgccac cggccgcgca gcgagcgcga cgtcgagcgc tccgtcgtgt
      421 tggtcgagcg cgaacgtgat gaacccgatg ccgacccgtg ccccggcggc cgcgtcgatc
      481 tcccgttcca gccacgccac atcgccgtac ccgccaccga tcagcccgaa tccaccgccg
      541 gccgacaccg ccgccgccag acgtcccccc gccgtgtccc ccatcggcgc caacaacacc
      601 ggatgccgaa cccccaacaa ctccgtcacg cgagtccgaa tcacctgccc cacagtaagc
      661 ggcttggcgc aaccgagtgg agttcggggt cgcttcccct ccgggtcgcg cggcgttgcc
      721 ccggcgagca ccgcgccttc gggcgcgggg tgaccggcac ggtcacacgt gtgggccgcg
      781 ccgaccgtgg cacaatggcc cgatggggtg catccggcgg ccatgaggat cacgcgcaag
      841 gccgactatg cggtgcgggc gatggtcgcc ttggctcgcg ccaacgcgcg ctacgacggc
      901 gtcgtgattc cccgcgacct catcgcgacg caggagacga ttccgcccgc gtacctcgac
      961 gacatcctgc gcaaactgcg caacggcggt ctggtgcgga gttgccgcgg cgtcgacggc
     1021 ggttggagca tcaacctcga cccggccgag atcaccgtcg gtgacgtgct gcgcgtgatg
     1081 gaaggtccnn nnnnnnnnnn ggcgggttgc gccccgagga actggcggac cagggcaccg
     1141 acgagtcgat ggtcgagctg tggtcggcga tgcgctcgtc gttgcgatcg gtgctcgacg
     1201 gggtcaccat tgccgacctt gccgccgggt tgctcccggc cgcaatcagc gaccttgcca
     1261 gtcgcagcgc tggctccgcc cgcacgatct gactgtcgcg accggctcct gcccacgcca
     1321 caccaggacg aggttgcggc aggactgccg tccccctaga ttgtcgacca atccggtcat
     1381 gaatgctcgg attaaacctg gtatttccac ccaggttttc agactttgac cacgcctcgg
     1441 gagccgagtt cgacatgacc acatctgtgt ggaggcgcgc cgcctccctc gccgccgcca
     1501 ccatctccct cgtgggcctc gccgcgtgcg gggacgacga cgcacccgcg tcggccgact
     1561 cgaccagcca ggcgccggtg atccgcgtcg gggccaccct gtcgctcacg ggcgacttcg
     1621 ccccggactc gaagctggtc cgcgacggct accagttctg gtacgagcag gtgcaggctg
     1681 ctggcgggat caccgttggc gacaccacct accaactcga actgatcacc caggacgacg
     1741 gttcgaaccg tgagcagggt gtgcagaacg tcgagcagtt catcaccatc gacgaggtcg
     1801 acttcgtcct ggccccgtgg ggatcgggca acaccgcggc ggtcgcccca gtgaccgaac
     1861 gttacgaaaa ggtcaccatc gctccgcttg cgatggctga ttcgctgtgg gagcagggcc
     1921 acggtcacct ctacggcgtg ttgccgttgg cctcggacgg catgcgtccg gtggtgcgct
     1981 tggccgagag cctcggcgcg acctcggttg cggtggtcgg caccgacgac ttgaacaaca
     2041 ccctgtcggc ccagggcgcc gtggacgagg ccacagcgct cggcctcgac gtgcggctgc
     2101 acgagaccta cccagaaggc cagatcgacc tcgccgccct gatctcgaag ttgaagaacg
     2161 ccgacgccga tctggtgatc gcggtggcgc acacgtcgga tgccgagttg ttgttgcgcc
     2221 agttcgaaga gcagcgctac ttcccgcggg cgctcagcct caccggcgcg gtcatcgtgc
     2281 ccgagttcct cgagtcgacc gagtacgtgt tcgggcagac gttctggtca ccggacctgc
     2341 cggtgcaaga cccgctgttc ggcaccgcgg gtgagttcgc gacggcgttc gaggagcggt
     2401 tcggctatcg gcccacgcac gactcggtgg cctcggcgac ggcgggttac ctgctgcaat
     2461 acgcgatcga gcaggccggc accctcgaca gcgcggctgt gaacgcggtg ttgcagacgc
     2521 tcggcatcga gacggtgctc ggtccggtga gcttcaccgc cgacacccac accaacgtcg
     2581 gtggaggcag ctacgtggtg cagatccaag acggtgtgcc cacgatcgtg taccccgccg
     2641 acctcagccg agccgcgccg atctaccccg ctccgggttg ggaccagcgt tgacacaagc
     2701 gctcgtcaac ggggtgctga ccggcgggct gtacgccgcg ctcggcgtcg gcctgtcgct
     2761 cgtgttcggc gtcaccaagg tgatcaacgc cgcccacgga gcgttgatcc tgnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cggctacggg ttgcagcgcg
     2941 tgctggtcaa ccgggtgctc gtccgcaacg tgtcgatgac gatggtgatg accctcggcg
     3001 tgagcttcgt gatctcgacc gtgctcgcct tcgtgtgggg cccggatcga cgctcgatca
     3061 cggtcggcta cgcagcgtcg ggattcgatc tgttcggcgc acgcgtgccg gtgacacggc
     3121 tcgcgatctt cgtgctctcg gtcgcgctca cctgcgggct ccacgcctac ctcacgcggg
     3181 ccgaaggcgg tcgccgcatt caggccgtgt cacaggaccc tatcgcggcg gagtcgatcg
     3241 ggctctccgt cgcccactac tacggcctct cccacgccct ggccgccgcg atcgcgctgg
     3301 ccgtcggcgg tgtcgtgccc ctcacccagc cggtgcaccc gttcatctcg gtcgacctgt
     3361 tgctgatcgc gttcgcggtg gtggtgctcg gcggattcgg atcgatcgtc gcgatcttgc
     3421 cggccggcat cgtgctggcg ctcggcgaga acttggaggg ttacctcatc ggactcaagt
     3481 accagcggtt ggcgatcttc gcggtgtacc tggcgatcgc gaccctgcga ccccagggcc
     3541 tgttcgggcg gcgtctctat gctcgttgac gttgcggtga gcgctcggtc ctcgacggtg
     3601 cgccgggtgc accgcgttcc ccgcggtggc gcgttggtga tcgcagtggc ggccgtgttc
     3661 gccgccccca ccgtgctgcc cgacaactac tggctgcgct tcgcgacgtc gacgctgatg
     3721 ctggccgcgc tggcgcagtc gctcaacatc acgtacgcga tgagcggata cgtgccgctc
     3781 ggtcatctcg tgttcttcgg cagcggtgcc gacggcgcag cgatcatcac ccnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnatcgcgg cgatcacctc
     3901 gcgggcgttg ctcggactcc gcagtgcgta cttcctcatg gcgaccgtgg ccctcaacgg
     3961 cctcgtcggc gaggccaccg tggtcttcga cggcctcacc ggtgggccac acgggatcat
     4021 cgtcgcgcca tgggtgcggg gcacgccgac ccacgtctat ctcgtcttct attacgcgat
     4081 ggctgccgcc gtggtggtga tcggcacggt gtcgatcatc atccgccgga gccgcctcgg
     4141 cgtcggcttg atggcgatca agggcaacga agacgcggcg gacgcgttcg gtgtaccaac
     4201 ccagcgctac aagacggcgg tgtggatcac gaccgcggnn nnnnnnnnnn nnnnnngccg
     4261 tcggcggcgt atacgcccac tggatcgggt acatcgacgc gtcgacgtcg ttcgacctgg
     4321 tgttctcgat cgaggtgttc ctggtggcgc tgatcggcgg acgctggttc gtcgtcggcc
     4381 cgctgatcgg cgcgctcgtg ttccgctacg tggaagacgt ggccgtgcgc aacaccgtcg
     4441 aggcgcagtt cggcgtgctc ggcgcggtgc tgatcgtgct cgtgctg
//
LOCUS       DAORNF010000203         4486 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_14412_length_4486_cov_1.760776, whole genome shotgun sequence.
ACCESSION   DAORNF010000203 DAORNF010000000
VERSION     DAORNF010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4486
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14412_length_4486_cov_1.760776"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..998)
                     /locus_tag="PLV68_09220"
     CDS             complement(<1..998)
                     /locus_tag="PLV68_09220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF and EAL domain-containing protein"
                     /protein_id="HRE01469.1"
                     /translation="MTGVITNLIHPDDRYALRRHVDEHVVELRSADSVAQEDLRLEHR
                     CVRADGQIVWVVTSIGLSSDAGGNEMAIVHMQDVTEQRQAAAQLQWAASHDELTGLPN
                     RTRFLDQLARRLEGAEAGAITVLFIDIDNFKVINDSLGHAAGDDLLRGMSDRLRQVVG
                     DRDLLGRFGGDEFMMMVDRASIPDGIESLAERVRAAIAQPLIVDGAELFVTGSIGMAS
                     ADRLGLHADELLRDADAAMYRAKTNGRDRVESFAPGEHEVSVIALRTASELRHGLERG
                     EVVPYFQPIVDLASGELTGFEVLARWRHPDRGLLTPDQFLPMAEESGLIGELGAAIL"
     gene            complement(995..>1317)
                     /locus_tag="PLV68_09225"
     CDS             complement(995..>1317)
                     /locus_tag="PLV68_09225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HRE01470.1"
                     /translation="KPIFALRRPDGTEVMVEADVRTPITNPDRIVATLRDITSRLRET
                     EELARAQVRFGLAFRSAPTGMLLATTDDEVVIDVNDALAAMLGYSRAEIIGRQTRALI
                     HRTP"
     assembly_gap    1318..1415
                     /estimated_length=98
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1416..2716)
                     /locus_tag="PLV68_09230"
     CDS             complement(<1416..2716)
                     /locus_tag="PLV68_09230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HRE01471.1"
                     /translation="MVVDDGIAATKAGPLLRASATTSRWIAVAVAVVAAVALDVIEAV
                     RSGTLAGSGRGLTDVSELVLPVLATALFVARWRNGRRAAALSLAVEPDMTSEADARDE
                     LIESGVGPRFDDTVVGTSAIAVARASRHRADGWLWLAAASLSWALGHLLWVVIAVTSD
                     RDPSASAPSVLFVVTALATARGLVKICGLTRAWPVVRPLVGDAAILAFSMGFLVWELF
                     LAPDVTDESTVTVLALAAVPLASTLVVAIGIVLLIDHRSLVVGAVTASAALLMIGDSA
                     LTAYFDNTSTTYASAHVLYLVAFALFAAAALLPHRLGRQPKRRSLARVSVVVVPAFVA
                     IGVGVLRYMVLHSKGNVVSTVLIATTGLAWVFAEVSRWVEARAMARELDAQIDQVRLA
                     QRDLRGLLDDLPEAVAVLSGDGRIVDVNGQALALTGRAHHEL"
     gene            complement(2784..>3581)
                     /locus_tag="PLV68_09235"
     CDS             complement(2784..>3581)
                     /locus_tag="PLV68_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HRE01472.1"
                     /translation="SAERIRDELDKLITVEHPSAGLFFLVDTGLADQFLPELPGMRLE
                     QDPIHRHKDVLTHTIAVVENVSRDFPVEFDFRVTRLAALMHDVGKPRTRGYLKGKGVT
                     FHHHEVVGARMTRTRLQALRYSTADVEAVTQLVHLHLRFHGYDTGWTDSAVRRYVVDA
                     GPLLNELNVLTRCDCTTRNERKAAMLSRRMDDLERRIAELRAKEELDAIRPEMNGQEV
                     MAHLGLAPGRAVGQAMKFLLDIRLEEGLLGEAAIRARLDEWWATHPH"
     assembly_gap    3582..3596
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3597..>3917)
                     /locus_tag="PLV68_09240"
     CDS             complement(<3597..>3917)
                     /locus_tag="PLV68_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HRE01473.1"
                     /translation="EITTHRAEMYADDSRKPHVTFSDEIESDLSRRDFTVNAMALELT
                     TDSPVLVDPFGGASDLVTRTLRTPLSPQISFGDDPLRMLRAARFIAGKHLQPVPELVE
                     AVVAM"
     assembly_gap    3918..3966
                     /estimated_length=49
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3967..4188)
                     /locus_tag="PLV68_09245"
     CDS             complement(<3967..4188)
                     /locus_tag="PLV68_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HRE01474.1"
                     /translation="MVPERFAPVLAELQPLAARFRDAGHRLYLVGGTVRDLLAGVPPD
                     EADFDATTDARPDRIKALLDGWADSLWNQG"
     gene            complement(4270..>4486)
                     /locus_tag="PLV68_09250"
     CDS             complement(4270..>4486)
                     /locus_tag="PLV68_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HRE01475.1"
                     /translation="GNYNAHLSAYPDFDWETFNGRFIESLGLTFNPYTIQIEPHDAMA
                     ELYDLTARANTIVLDTCRDIWAAIDQF"
BASE COUNT          701 a         1462 c         1500 g          661 t
ORIGIN      
        1 aggatcgcgg cgccgagctc gccgatcaag ccgctctctt cggccatcgg caggaactga
       61 tcgggtgtca gcaggccgcg gtcggggtgg cgccagcgag ccagcacctc gaacccggtc
      121 agttcgccac tggcgagatc gacgatcggc tggaagtagg gcacgacctc gccccgctcc
      181 agaccgtggc gcagctcgct cgcggtacgc agggcgatga ccgacacctc gtgttcgcca
      241 ggagcgaacg actcgacccg gtcgcggccg ttggtcttgg cccggtacat cgccgcgtcg
      301 gcatcacgca gcaactcgtc cgcgtgcagg cccagccggt cggccgaggc catgccgatg
      361 ctgccggtga cgaacagctc ggcaccgtcg acgatcagcg gttgggcgat cgcggctcgc
      421 acccgttcgg cgagcgattc gatgccgtcg gggatgctgg cccgatcgac catcatcatg
      481 aactcgtcgc caccgaagcg accgagcagg tcgcggtcgc cgacgacctg gcggagacgg
      541 tcgctcatcc cgcgcagcag gtcgtcgccc gcggcgtggc cgaggctgtc gttgatgacc
      601 ttgaagttgt cgatgtcgat gaacagcacc gtgatcgcgc cagcctcggc gccctcgagg
      661 cggcgcgcca actggtcgag gaagcgggtg cggttgggta gcccggtgag ctcgtcgtgg
      721 ctggccgccc actgcagctg ggcggccgcc tggcgctgtt cggtcacgtc ctgcatgtgg
      781 acgattgcca tctcgttgcc gcccgcgtcg ctggacagac cgatcgaggt caccacccac
      841 acgatctgac cgtcggcgcg cacgcagcgg tgctccaggc gcaggtcctc ctgggccacg
      901 ctgtcggccg agcgcagttc gacgacatgt tcgtccacgt ggcggcgcag tgcgtagcgg
      961 tcgtcgggat ggatcaggtt ggtgatgacc ccggtcatgg tgttcgatgg atcagagcgc
     1021 gggtctgacg cccgatgatc tcggcccgcg agtaccccag catcgcggcc agcgcgtcgt
     1081 tgacgtcgat caccacctcg tcgtcggtcg tcgccaggag catgccggtc ggcgccgagc
     1141 ggaaggccaa cccgaaccgg acctgtgccc gggccaactc ttcggtctcg cgcagccggc
     1201 tggtgatgtc gcgcagtgtg gccacgatgc ggtctgggtt ggtgatcggt gtgcgcacgt
     1261 cggcttcgac catcacctcg gtgccgtccg ggcgccgcag ggcgaagatc ggcttcgnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnagttc gtgatgggca cggccggtca
     1441 acgccagcgc ctggccgttg acgtcgacga tgcggccgtc ccccgacagc accgccaccg
     1501 cttcgggcag gtcgtcgagc aagccgcgca ggtcgcgctg ggcgaggcga acctggtcga
     1561 tctgcgcgtc gagctcgcga gccattgccc gcgcctcgac ccaccggctg acctccgcga
     1621 acacccacgc cagcccggtc gtggcgatca gcaccgtgct gaccacgttg cccttggagt
     1681 gcaacaccat gtagcgcaac acgccgacgc cgatcgccac gaacgccggc acgaccacca
     1741 ccgacacccg ggccaggctg cgccgcttgg gttgacggcc gaggcggtgg ggcaacaacg
     1801 ctgccgctgc gaacagcgcg aacgccacca gatacagcac gtgcgcgctg gcgtaggtgg
     1861 tggacgtgtt gtcgaagtac gcggtcagcg ccgagtcacc gatcatcaac agcgcggccg
     1921 aagccgtcac cgcgccgacc accagcgagc gatggtcgat cagcaacacg atgccgatcg
     1981 cgacgaccaa cgtcgaggcc agcggcaccg cggcaagggc cagcaccgtc acggtcgact
     2041 cgtcggtgac gtcgggcgcg aggaacaact cccacaccag gaaccccatc gagaaagcga
     2101 ggatcgccgc gtcgcccacg agcggacgga ccaccggcca ggcgcgggtc aagccgcaga
     2161 tcttgaccag gccgcgggcg gtggccagcg cggtcaccac gaacagcacc gacggtgccg
     2221 acgcgctggg atcgcgatcc gacgtgacgg cgatgaccac ccacagcagg tggccgagcg
     2281 cccagctcag ggacgcggct gccagccaca gccagccgtc ggcgcggtgg cgactggcgc
     2341 gggccacggc gatcgcgctc gtgccgacca ccgtgtcgtc gaagcggggt ccgacgccgg
     2401 actcgatcaa ctcgtcgcgc gcgtccgcct ccgacgtcat gtcgggctct acagccaggc
     2461 tcagcgccgc ggcgcggcga ccgttacgcc agcgggccac gaacaacgcc gtcgccagca
     2521 cggggagcac caactcggac acgtcggtca aaccccggcc cgagcccgcc aaggtgcccg
     2581 agcgcaccgc ctcgatcaca tcgagagcga ccgccgccac gacggcgaca gcgacggcga
     2641 tccacctgga cgtcgtcgcg ctggcgcgca acaacggccc tgccttcgtc gccgcaatgc
     2701 cgtcgtcaac caccatcttt ttcgtttcac cttgtactgg cgacccaccg tatcaacccc
     2761 accgctcgca gtggtgttct ccctcagtgg gggtgggtcg cccaccattc gtcgagacgg
     2821 gcacggatgg ctgcttcgcc gagcaggccc tcttcgaggc ggatgtcgag caggaacttc
     2881 atcgcttggc cgaccgcacg gccgggggcc agaccgaggt gggccatgac ctcttgaccg
     2941 ttcatctcag ggcggatcgc gtcgagctct tccttggcgc ggagctcggc gatgcggcgc
     3001 tccagatcgt ccatccgtcg cgacagcatc gccgccttgc gttcgttgcg agtggtgcag
     3061 tcacagcgcg tcagcacgtt gagttcgttc aacaacggcc cggcgtcgac cacgtagcga
     3121 cgcaccgccg agtcggtcca tccggtgtcg tagccgtgga agcggaggtg gaggtggacc
     3181 aactgggtca ccgcctcgac gtcggcggtg ctgtagcgca acgcttgcag gcgcgtgcga
     3241 gtcatgcgcg cgccgaccac ctcgtgatgg tggaaggtga cccccttgcc cttcaggtag
     3301 ccccgcgtgc gcggcttgcc gacgtcgtgc atcagcgcgg ccaggcgcgt cacacggaag
     3361 tcgaactcga ccgggaagtc acgggagacg ttctcgacca cggcgatggt gtgggtgagc
     3421 acgtccttgt ggcgatggat cgggtcttgc tccaaccgca tgcccggcag ttccggaagg
     3481 aactgatcgg ccagaccggt gtcgaccagg aagaacagac cggccgacgg gtgctcgacg
     3541 gtgatcagct tgtcgagctc gtcgcggatg cgctcggccg annnnnnnnn nnnnnncatc
     3601 gccaccactg cctccaccag ttcgggcacc ggctggaggt gtttgcccgc gatgaaccgg
     3661 gcggcgcgca acatgcgcag cgggtcgtcg ccgaagctga tctgcgggct gagcggcgtg
     3721 cgcagcgtgc gcgtgaccag gtcggacgca ccgccgaagg ggtcgaccaa caccggcgag
     3781 tcggtggtga gctcgagcgc catcgcgttg acggtgaagt cgcgccgcga cagatcggac
     3841 tcgatctcgt cggagaacgt gacgtgcggc ttgcgcgagt cgtcggcgta catttcggcg
     3901 cggtgggtgg tgatctcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnngccc tggttccaca gcgagtcggc ccatccgtcg agcaacgcct tgatccgatc
     4021 ggggcgggcg tcggtggtgg catcgaagtc ggcctcgtcc ggtggcactc cggcgagcag
     4081 gtcgcgcacg gtgccaccga ccaggtacaa ccgatggccc gcatcgcgga agcgtgccgc
     4141 cagcggttgc agctcggcca acacaggagc aaaccgttca ggcaccatgg tcgccccagg
     4201 ctaatgacca gtaccgctgg agcatcctgc gggtttggct cggcgtctga tgtcgccggg
     4261 ctcgcgccgt cagaattggt cgatcgcggc ccagatgtcg cggcaggtgt ccagcacgat
     4321 ggtattggcg cgggcggtga ggtcgtagag ctcggccatc gcgtcgtggg gctcgatctg
     4381 gatggtgtag gggttgaagg tgaggcccag ggactcgatg aagcgaccgt tgaaggtttc
     4441 ccagtcgaag tcggggtagg ccgacaggtg ggcgttgtag ttgccc
//
LOCUS       DAORNF010000204         4482 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_14435_length_4482_cov_1.787667, whole genome shotgun sequence.
ACCESSION   DAORNF010000204 DAORNF010000000
VERSION     DAORNF010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4482
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14435_length_4482_cov_1.787667"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>240
                     /locus_tag="PLV68_09255"
     CDS             <1..>240
                     /locus_tag="PLV68_09255"
                     /inference="COORDINATES: protein motif:HMM:NF012490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HRE01476.1"
                     /translation="KRLGVLAGDFGGFPNGRRPGDDVVDIQRRLLPAERHLIRRLVAF
                     FATGDSIVANNLVLNLYAHINSPEARLFLSRQLYE"
     assembly_gap    241..295
                     /estimated_length=55
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <296..935
                     /locus_tag="PLV68_09260"
     CDS             <296..935
                     /locus_tag="PLV68_09260"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HRE01477.1"
                     /translation="AEAFAAVENIPSVRAKADFCFRWMESMSKLQRLETAEDRRTFLL
                     NLICFAACIEGLFFFGAFAYVYFLRSKGLLNGLAAGTNWVFRDESGHMDFAFAVVDTI
                     RREEPELFDADLAAQVTAMMEDAVATEMVFVADMLSEGVPGLSPADTRTYLEYVADQR
                     LVRLGIPKRYGSKNPFGFMELQDVQELSNFFERTVSSYQIGVTGEVAFDEDF"
     gene            1090..>1300
                     /locus_tag="PLV68_09265"
     CDS             1090..>1300
                     /locus_tag="PLV68_09265"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRE01478.1"
                     /translation="MTTSSRADAPAVLAEGLVKRFGETEALAGVDLRVERAKVVGLLG
                     PNGAGKTTTVRVLATLIRPDAGRAFI"
     assembly_gap    1301..1330
                     /estimated_length=30
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1331..2110
                     /locus_tag="PLV68_09270"
     CDS             <1331..2110
                     /locus_tag="PLV68_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRE01479.1"
                     /translation="IGLTGQYAAVDERLTGFENLEHVGRLFHLGRREARQRGRELLDR
                     FALSDAGDRVVKGYSGGMRRRLDIAMSLIARPSVLFLDEPTTGLDPRSRQSVWELIEE
                     LVAAGTTTLLTTQYLDEAERLADDIVVIDHGRVIATGTSEELKRQVGGDVLEVLFADE
                     LSARAAATELAPVAIGEILVAANESGDGVTVTVPVTRALGAGPGIIRRLDAAGIAVVD
                     VASRTATLDDVFFALTGHGADQTTTDADQDTNPQRATKGDS"
     gene            2107..2940
                     /locus_tag="PLV68_09275"
     CDS             2107..2940
                     /locus_tag="PLV68_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRE01480.1"
                     /translation="MTTISPMPPAAGADAIGQHEPATGLSWALRDAWTEASRHLRALP
                     RNPELLIFATLQPIMFIVLFVYVFRGSVQVPIDYVQYVIPGIFAQTVLFNASFTAVGL
                     ADDLSKGIIDRLRSLPMYPSAVLVGRTLSDIVRNVITFAVMLAVAFAIGFRFEGSLLG
                     ALGATGLMMLFSYAFSWIQALIGLSVGSVEAANSAGFIWMFPLTFVSSAFVDVNLMTP
                     WLRRIARNNPFTVLTNACRALYNGQDPGSDLWVAIGWAVGIILVFATLATRKYARSTS
                     A"
     gene            complement(2968..>4482)
                     /locus_tag="PLV68_09280"
     CDS             complement(2968..>4482)
                     /locus_tag="PLV68_09280"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRE01481.1"
                     /translation="FVDLHTHLDAQVAWDPLMTSSSWHGVTTVLMGNCGVTFAPVAPG
                     DKVFLAEMMESVEDVPRDAILNGMPWDWETYPQYLDSVERMRPAVNVVGMVGHCAVRY
                     HVMGERSLGDTPATAAELDQMRAVAAESVAGGAVGFSTSRILLHTIPDGRYVPGTLAP
                     LDEYLAIADGMNEGGGGLFQAVNDFATKAQHEIELLRQMAGACGKVLFSGGPGNGTGT
                     AGAEMFGAFLDDVNRTTGQMTMLTQTRPSGTLCGLAQVSPVKGKQWKALMGLPTLADR
                     VAALRDPAIRAELAAEGYEKGMWYDPTLIYPLGTGPSPDYSETGGMSIAQRAERLGIH
                     PVDFVIDRLIASDGLELFNTWFFSRNLAALEMLLQTEHVVPGLADTGAHAGQICDADM
                     GTHYLYYWHRDRGINTLPQAIRNLTGVPAAVLGLRDRGTLTVGSHADINVFDLDGLAS
                     QHPRFDNDFPGGAGRLKIGSRGYAATFVNGEVIAEHGVNTGARPGRVLRDFART"
BASE COUNT          683 a         1503 c         1474 g          737 t
ORIGIN      
        1 caagcgtctg ggcgtcctgg ccggtgattt cggtggcttt cccaacggcc gtcgtccggg
       61 cgacgacgtg gtcgacatcc agcgccgcct gctccccgcc gaacgccacc tcatccgccg
      121 tctcgtcgcg ttcttcgcca ccggcgactc gatcgtggcc aacaacctgg tgctcaacct
      181 gtacgcgcac atcaactcgc ccgaagcgcg tctgttcttg agccgccagc tgtacgaaga
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnggccg
      301 aggctttcgc cgcggtcgag aacatcccct cggtgcgggc caaggccgac ttctgcttcc
      361 gctggatgga gtcgatgtcg aagctccaac ggctggagac ggccgaggac cggcgcacgt
      421 tcttgctcaa cctgatctgc ttcgcggcat gcatcgaagg actgttcttc ttcggcgcgt
      481 tcgcgtacgt gtacttcctg cgttccaagg gtctgctcaa cggtctggcc gcggggacga
      541 actgggtgtt ccgcgacgag agcgggcaca tggacttcgc gttcgccgtg gtcgacacga
      601 tccggcgtga agagcccgag ctgttcgacg ccgatttggc ggcccaggtc acggcgatga
      661 tggaggacgc cgtggcgacg gaaatggtgt tcgtcgccga catgctgtcc gaaggcgtgc
      721 ccggcctgtc gccagccgac acccgtacgt atctcgagta cgtcgccgat cagcggctgg
      781 tgcgcctcgg catccccaag cgctacggca gcaagaaccc gttcgggttc atggagctgc
      841 aagacgtgca ggagctgtcg aacttcttcg aacgcacggt cagcagctac cagattggtg
      901 tcaccggtga agtcgccttc gacgaagact tctgacgcgc tcggtcgccg agcgcgagcg
      961 tcgacgatgg gttgacgcag gacatcgtca aggtgggggc gcaaaaccct gagcgccgcc
     1021 ggttcgtacg cgctagacag gaaagccaca cccacacgcc ccgcgtaccc cacaactgcg
     1081 gagaccccca tgaccacatc ttcacgcgcc gacgcgccag cggtgctggc cgaggggctc
     1141 gtcaaacgct ttggcgagac cgaggccctc gccggcgtcg acttgcgcgt cgaacgtgcc
     1201 aaggtcgtcg gcctgctcgg tcccaacggg gcgggcaaga ccacgaccgt gcgcgttctc
     1261 gccacgctga tccgccccga cgccggccga gcgttcatcg nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn atcggcctga ccggccagta cgcggcagtc gacgagcgcc tcaccggctt
     1381 cgagaacctg gagcacgtcg ggcggctgtt ccacctgggc cgtcgagagg cgcgccagcg
     1441 cgggcgtgag ctgctcgacc ggttcgcgct gagcgatgcc ggagaccggg tcgtcaaggg
     1501 ctactcgggc ggcatgcgcc gccggctcga catcgcgatg agcctgattg cccgcccgtc
     1561 ggtcctgttc ctcgacgagc cgaccaccgg cctcgatccc cgcagtcgcc agtcggtgtg
     1621 ggagttgatc gaggagctcg tcgcggccgg cacgaccacg ctgctgacta cccagtatct
     1681 cgacgaggcc gagcgtctgg ccgacgacat cgtcgtcatc gaccacggcc gcgtgatcgc
     1741 cacgggcacc agcgaggagt tgaagcgtca ggtcggcggc gatgtgctgg aggtgctgtt
     1801 cgcggacgag ctcagtgccc gcgccgcagc gaccgaactg gccccggtgg cgatcggcga
     1861 gatcctcgtc gcggcgaacg aatcgggcga cggcgtcacc gtcaccgtgc cggtcacccg
     1921 tgccctcggg gcgggacccg gcatcatccg ccgcctcgac gcagcgggca tcgcggtggt
     1981 cgacgtcgcc agccgcaccg cgaccctcga cgacgtgttc ttcgcgctga ccggccacgg
     2041 cgccgaccag accaccaccg acgctgacca ggacaccaac cctcagcggg ccacgaaggg
     2101 cgactcatga cgaccatctc accgatgccc cctgcggccg gagccgacgc gatcggccag
     2161 cacgaaccgg caaccggtct gagctgggcg ttgcgcgacg cctggaccga agcgtcccga
     2221 cacctgcgcg ccctgccgcg caatcccgag ctcctgatct tcgccacgct ccagccgatc
     2281 atgttcatcg tcctgttcgt gtacgtgttc cgcggctcgg tgcaggtgcc gatcgactac
     2341 gtgcaatacg tgatcccggg catcttcgcc cagacggtgc tgttcaacgc cagcttcacc
     2401 gcggtcggcc tggccgacga cctgagcaag ggcatcatcg accgcctccg ttcgttgccg
     2461 atgtatccgt cggcggtgtt ggtggggcgc acgctgagcg acatcgtccg caacgtgatc
     2521 acgttcgcgg tgatgctcgc ggtcgcgttc gcgatcggct tccgcttcga aggctcgctg
     2581 ctcggcgcgc tgggcgcgac cggcttgatg atgctgttct cgtacgcgtt cagttggatt
     2641 caagccttga tcgggctgtc ggtcgggtcg gtcgaggcgg ccaactcggc cggcttcatc
     2701 tggatgttcc cgctgacgtt cgtgtcgtcg gcgttcgtcg acgtcaacct gatgaccccg
     2761 tggcttcgcc ggatcgcccg taacaacccg ttcaccgtgc tgaccaacgc ctgccgcgcg
     2821 ctctacaacg gccaggaccc ggggtctgac ctgtgggtcg cgatcggctg ggcggtcggc
     2881 atcatcctgg tgttcgccac gctggccacc cgcaagtacg cccgctcgac cagcgcctga
     2941 caccaggagc tgttgggggc cgacggctca ggtgcgggcg aagtcgcgca gcacgcggcc
     3001 gggccgggcg ccggtgttga cgccgtgttc ggcgatcacc tcgccgttga cgaacgtggc
     3061 ggcgtagccg cgcgagccga tcttgagccg cccggcacca ccggggaagt cgttgtcgaa
     3121 gcgggggtgc tgtgacgcca acccatcgag atcgaacacg ttgatgtcgg cgtggctgcc
     3181 gacggtgagc gtgccacgat cgcgcaaccc gagcaccgcc gcgggcactc cggtgaggtt
     3241 gcggatcgcc tgcggcagcg tgttgatgcc gcggtcgcgg tgccagtagt agaggtagtg
     3301 ggtgcccatg tcggcgtcac agatctggcc cgcgtgggcg ccggtatcgg ccaggccggg
     3361 aacgacatgt tccgtttgca acaacatctc caacgcggcg agattgcggg agaagaacca
     3421 ggtgttgaac agctccagac catcgctggc gatcaggcga tcgatcacga agtcgaccgg
     3481 gtgtatgccc aaccgctcgg cacgttgggc gatcgacatg ccaccggtct cgctgtagtc
     3541 gggcgatggg ccggtgccga gcgggtagat cagcgtcggg tcgtaccaca tgcccttctc
     3601 gtagccctcg gccgcgagtt cggcgcggat cgccgggtca cgcagggcgg cgacgcggtc
     3661 ggccagcgtg ggcagaccca tgagcgcctt ccattgcttg cccttcaccg gcgacacctg
     3721 ggccagaccg cacaacgtgc cgctggggcg ggtctgggtc agcatcgtca tctggcctgt
     3781 ggtgcggttg acgtcgtcga ggaacgcgcc gaacatctcc gcgcccgcgg tgcccgtgcc
     3841 gttgccgggg ccgccgctga acagcacctt gccgcacgca cccgccatct ggcgcagcag
     3901 ttcgatctcg tgctgagcct tggtggcgaa gtcgttgacc gcctggaaca gcccaccgcc
     3961 gccttcgttc atgccgtcgg cgatggcgag gtactcgtcc agcggggcca gcgtgcccgg
     4021 cacgtagcgg ccatcgggaa tcgtgtgcaa caggatgcgc gacgtcgaaa agcccaccgc
     4081 gccgcccgcg accgactcgg ccgccacggc acgcatctgg tcgagctcgg cagccgtcgc
     4141 cggggtgtcg ccgagcgaac gttcgcccat cacgtggtag cgcacggcgc agtggccgac
     4201 catgccgacc acgttgaccg cgggccgcat ccgctcgacc gagtcgaggt attgcgggta
     4261 ggtctcccag tcccacggca tgccgttgag gatcgcgtca cgcggtacgt cttcgacgct
     4321 ctccatcatc tccgccagga agaccttgtc gcctggggcg acgggggcga aggtgacacc
     4381 gcagttgccc atcagcaccg tggtgacgcc gtgccacgaa ctggacgtca tcagcgggtc
     4441 ccacgcgacc tgggcgtcga ggtgggtgtg caggtcgacg aa
//
LOCUS       DAORNF010000205         4464 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_14525_length_4464_cov_2.038331, whole genome shotgun sequence.
ACCESSION   DAORNF010000205 DAORNF010000000
VERSION     DAORNF010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4464
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14525_length_4464_cov_2.038331"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /locus_tag="PLV68_09285"
     CDS             complement(<1..352)
                     /locus_tag="PLV68_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01482.1"
                     /translation="MLVRVAVGVFVRVAVAVGVLVREGVAVGPVGVFVRVAVGPAGVF
                     VRVAVAVAVRVAVAVRVAVEVAVAVEVAVGAGTVGVFVGATPPARSETIQAGSALSAP
                     AAVPSVPVLPLAEPS"
     gene            426..1010
                     /locus_tag="PLV68_09290"
     CDS             426..1010
                     /locus_tag="PLV68_09290"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HRE01483.1"
                     /translation="MVRAGAIPGTGPLTIEAWVRPGAANESGIMLASGDDFTGWSLEL
                     ENGRLTAWLSTNQGWQPNQHPTVLVAGQWYHVAMTVGGGSLRTFVNGVASTATSVGTL
                     TQGPATTIGGLTGYAYYNGAIDEIRASNVLRYSGAFTPPATLTNDANTVALWRFNELS
                     GQTTADGSASGNTGTLGTAAGADSADPAWVTVVR"
     gene            1193..3469
                     /locus_tag="PLV68_09295"
     CDS             1193..3469
                     /locus_tag="PLV68_09295"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRE01484.1"
                     /translation="MSQSRQPRLFPGRFLNESLSGAVRVGLALAVGLASLVWPGGPGG
                     RVPLAMANGGIDGSFTQTTATDFSGACTALTGSIPTPVLASTTVSPVNGGELRLAATL
                     EDYFDGTEVDLTRWITGTAQAFYTSTVVVSGGYVILDGTYLRSVQNFQSTQPRFFEAR
                     VRNRVLETQSVGWTDVGFYREAPPLAGIFPATSAYRIFVTRDDGSVYGLARDGDGSVP
                     GVPTEIDPAPTAELFNIYRLEWDAGAVADTRFLVNDAPVGTLSGSSDLNTWAFLYQQE
                     DSFLATQYSQLYVDWVRAGQYATSGTYTSCARDAGGVVNWSTLTATTVTTTTTTAAFE
                     TRTSLDGTTWSSWEAVTGSTVASPSGRFFQYRTTLGSSNPYLSPEVAQVVVNYYGPSS
                     LQVTPTTISLDPGATQTFTVAAFDGNSRAVTGLNYTWGLNAGGGVLGATGVFTAGLPA
                     GTFANTVAVTVTTTGSPVSGSASVSVNDLPPTADAGGPYTVAEGSSVNVTGSGVDPNG
                     LPIGVSWDLDDNGSFETPGSPASFAGVDDGVYTVRLRALENGGAELLTVVTASITVTN
                     VAPVVTITGAPTTTVAEGTAVTLGQLVTDAGVADTHTFAWSVTKGGNPFASGSGASFT
                     FTPDDDGSYVATVVATDDDGGQDSEDVTVTVANVAPAVSITGAPASAGPGVPVTLGVT
                     TTDAGSADTHTYAWTVTKDGDPFATGSGASYTFTPDGAGVYEVTVEATDDDGGLATDT
                     VTIGGQYQLIIVAVMRDY"
     assembly_gap    3687..3821
                     /estimated_length=135
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3822..>4083
                     /locus_tag="PLV68_09300"
     CDS             <3822..>4083
                     /locus_tag="PLV68_09300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01485.1"
                     /translation="APAETPQPRSTKPQTIATATEGEAAAIVAPPVTSTARPTPLPAI
                     SGYSLRFFGTGRQDIDRVKLRLDPPVPADIGATDFTLEFWVKA"
     assembly_gap    4084..4245
                     /estimated_length=162
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          687 a         1367 c         1433 g          680 t
ORIGIN      
        1 ccgatggctc ggccagcggc aacacgggca cgcttggcac ggccgccggc gccgatagcg
       61 ccgacccggc ctggatcgtc tcggatcgtg cgggtggtgt tgctccgaca aacaccccga
      121 ccgtcccggc gccgaccgca acttcgacgg cgaccgcaac ttcgacggcg acacgcaccg
      181 cgacggcgac acgcaccgcg acggcgaccg caacgcgcac gaacacgcca gccgggccga
      241 cagcgacgcg cacgaataca cccaccgggc cgacggcgac gccctcgcgg accaacacgc
      301 ccacggccac ggcgacgcgc acgaacacac ccaccgccac ccgcaccaac acggcgacgg
      361 ctctgccgac cgttactcca acgctcggcg gcaacggcgg cctgcagttc gatggagtga
      421 acgacgtggt gcgcgcgggc gcgatcccgg gcacggggcc gctgacgatc gaggcctggg
      481 tgcggccggg cgccgccaac gagagcggga tcatgctggc gagcggcgac gacttcacgg
      541 gctggtcgct cgagctcgag aatgggcggt tgacggcgtg gttgtcgacc aatcagggct
      601 ggcagccgaa ccagcacccg actgtgctgg ttgccgggca gtggtaccac gtggcgatga
      661 cggtcggagg cgggagcctg cggacgttcg tcaacggcgt ggcgagcacg gcgacgagcg
      721 tcgggacgtt gacgcagggg ccggcgacga cgatcggcgg gctgacgggg tatgcgtatt
      781 acaacggcgc gatcgacgag atccgggcgt cgaatgtgtt gcgttacagc ggggcgttca
      841 cgccgccggc gacgctgacg aacgacgcga acacggtggc gctgtggcgc ttcaatgagt
      901 tgagcgggca gacgacggcc gatggctcgg ccagcggcaa cacgggcacg cttggcacgg
      961 ccgccggcgc cgacagcgcc gacccggcct gggtgacggt ggttcgctga gacacgcgcg
     1021 ttgttacgaa cgtagcacgg cgcgcctcgg ttgataggct gaaccgaggc gcgccatttt
     1081 gcttttgggc cagtggtacc gtggtacaaa gtgactagcg gtgcgcccga acctatccct
     1141 tacaattctt aacggtccac taacaacaac ccataaccaa ggtggtcttt ccatgagcca
     1201 atcccgtcaa ccccgtttgt ttccgggtcg gtttctgaac gaatctctta gcggcgcggt
     1261 ccgcgtcgga ttggcgttgg ctgttgggtt ggcgagcctg gtgtggccgg gcggcccagg
     1321 gggccgggtc ccgctggcga tggccaatgg cgggatcgat ggctccttca cccagaccac
     1381 ggcaaccgat ttctcaggcg cctgcaccgc gcttaccgga agcatcccga cgcctgtgct
     1441 cgccagcacc acagtcagcc ctgtgaatgg cggtgagttg cgccttgcgg cgaccctcga
     1501 ggactacttc gacggcaccg aagtggattt gacgcgttgg atcacgggca cggcgcaggc
     1561 gttctacacc tcgaccgtgg tcgtctccgg cggttacgtg atcctggatg ggacctacct
     1621 gcgttcggtg cagaacttcc aatccacaca gccgcgcttc ttcgaggcgc gggtgcgcaa
     1681 ccgcgtcctc gaaacacaga gcgtcggttg gaccgacgtg gggttttatc gtgaagcccc
     1741 gcctctggcc gggatctttc ctgccacgag tgcttatcgg atcttcgtga cacgtgacga
     1801 tggctctgtc tacgggctcg cgcgcgacgg cgatggcagc gttcccggtg ttcccaccga
     1861 gatcgacccg gcgcctacgg ccgagctgtt caacatctat cgcctggagt gggatgccgg
     1921 cgcggttgcc gatacgcgct ttctcgtcaa tgatgcgccg gtcgggaccc tctcgggctc
     1981 ctccgacctg aacacctggg cgttcctgta tcagcaagag gactcgttcc tggccaccca
     2041 gtattcgcag ctctatgtcg actgggtccg tgccgggcag tacgcgacaa gcggcaccta
     2101 tacgtcgtgc gcgcgcgatg cggggggtgt cgtcaactgg tcgaccctga ccgcgacgac
     2161 tgtgacgacg accacaacga ctgccgcgtt cgagacgcgc acctcactcg acggcacgac
     2221 ctggtcgagc tgggaggcgg tgaccggcag cacggtggcg agcccaagcg ggcgcttctt
     2281 ccagtatcga acgacgcttg gctcgtccaa cccgtatctc tcgcccgaag tggcgcaggt
     2341 ggtcgtgaac tactatggcc catcgtcctt gcaggtgacg cccacgacaa tctcgctcga
     2401 cccgggcgca acgcagacct tcacggttgc ggcgttcgac ggcaacagcc gcgccgtcac
     2461 gggcctgaac tacacctggg gcctcaatgc cggtggcggc gtgctgggcg ccacgggcgt
     2521 gttcacggct gggctgccgg ccggcacctt cgccaacacc gtcgcggtca ccgtaaccac
     2581 cacaggcagc cccgtaagcg ggtcggcctc ggtgtcggtc aacgacctgc caccgaccgc
     2641 agatgcaggc gggccatata ctgtcgccga gggcagctcg gtcaacgtga ctggctccgg
     2701 cgtggacccg aacggcctac cgatcggtgt gagctgggat ctggatgaca acggttcgtt
     2761 cgaaacgccc ggatcgccgg cgagcttcgc gggcgtcgat gacggcgtct acacggtcag
     2821 gctcagggcg ttggagaacg gcggagccga gttgctgacc gtcgtgacgg cgtcgatcac
     2881 ggtgaccaac gtggcgcctg tcgtgaccat cactggcgcg ccgaccacca cggtcgctga
     2941 gggaacggcc gtcacactgg gtcaactcgt cacggatgcc ggggtcgccg acacccatac
     3001 cttcgcctgg agcgtgacca agggtggcaa tcccttcgcc agcgggtcgg gcgcttcctt
     3061 cacgttcacg cccgacgacg atgggagcta cgtggccact gtggtggcga ccgacgatga
     3121 tggcggccag gacagcgagg atgtcacggt cactgtcgcg aacgtcgcgc ctgcggtttc
     3181 gatcaccggg gccccggcga gcgccggccc gggtgtgccc gtcaccctcg gggtgacgac
     3241 gaccgacgcc gggtcagcag acacgcatac ctacgcctgg acggtcacga aagatggcga
     3301 tccattcgcc acgggctctg gcgcttccta cacgtttacg ccggatggcg ccggggtgta
     3361 tgaggtgact gtcgaggcga ctgacgatga cggcggcctg gccacagaca cggtgacgat
     3421 cggcggccag tatcagttga tcatcgtggc tgtgatgcgg gattactgag gtgcagaccg
     3481 gcgtgcccgg cgccggatgg acatgattgc acttgctggc cgtcggcgcg cgacacgtga
     3541 cgccgacggc cagcgtcatg cccggcgcgg agctgagatc cacgaccctc atacggtggt
     3601 ggcgatcagc ccgactgggg gaggcgtctg ttgagcggtc tagcccgtta ttggagccgg
     3661 ggcgttgggt ggctcgccgt cctgttnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nggcgccggc cgagacgccc
     3841 cagccgcgct cgacgaagcc ccagacaatc gcaacggcga cggagggcga agcggccgcg
     3901 attgttgcgc cacccgtcac ctcgactgcg cgcccgacgc cactgccggc gatctccggc
     3961 tactcgctgc gcttttttgg gacaggccgg caggatatcg accgcgtcaa gctcaggctt
     4021 gatccgccgg tgcccgctga catcggagcg acggacttca cgcttgagtt ctgggtgaag
     4081 gccnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngcgaa gcgacgctct
     4261 gcggcaacag tgatctccgc gatggggcgt ggcaccatgt cgctgtgcaa cgtcgccgca
     4321 ccgatggata tctctggctg tatgtcgatg gtcgactcga ggccgaggtc gatgggcctg
     4381 atggcgatat ctcctacccc gacggcgcgg cgcccggccg gccgggcgct ccctggtgca
     4441 gcggccctgg gggggggtgg ggcg
//
LOCUS       DAORNF010000206         4459 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_14559_length_4459_cov_1.798819, whole genome shotgun sequence.
ACCESSION   DAORNF010000206 DAORNF010000000
VERSION     DAORNF010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4459
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14559_length_4459_cov_1.798819"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    215..385
                     /estimated_length=171
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <386..1497
                     /locus_tag="PLV68_09305"
     CDS             <386..1497
                     /locus_tag="PLV68_09305"
                     /inference="COORDINATES: protein motif:HMM:NF022169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2510 domain-containing protein"
                     /protein_id="HRE01486.1"
                     /translation="PDPDDTGGLRYFDGRAWSEHRARLHLPAPAPNQPTAAMCACGVV
                     AIGRCRVCAEAYCSAHISSTAVDDRPFRKRWEPWTCGNCIEDAQREIRALQLARCDNV
                     MMQMVRIPHLNRVKTPNGRRPPVISLFQHPRSPTERPPRYAQAYLVEYDGGPPNPTFE
                     GLALSIDGSTVFNIGLPVAGVARQRFGPKRTITGYIVKNVIDIDRLSDAAAQPTTESW
                     FEFAARSYLRCAHHIGIKPDFSLVFDPAPLPELEPASAAIADAEPHTVPARDMDHDEP
                     APAPTAANTSRSKRPKKPRAAATNPIDQPSDEPLISDDESRADGIPRPHVGDDGIIND
                     VAHVEVPAADSSPSQEPAVRQPDPPASPPLPLPEP"
     gene            complement(1543..2034)
                     /locus_tag="PLV68_09310"
     CDS             complement(1543..2034)
                     /locus_tag="PLV68_09310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01487.1"
                     /translation="MTSDEIRDELPEDLDASEFVGPYQFPDNSRRRIPGVLYLAIAVI
                     SFFAWLVTRNDTPVLINDGMLYGAILLAAFGAVCLTSGWRMHVDEKQALVAATSAVGF
                     AVGHASAQQVWRGLRSRPTWRVLCYSSENPPRRRGLVLVDAVDGTVLQHLVQDNPETD
                     WED"
     gene            complement(2041..2562)
                     /locus_tag="PLV68_09315"
     CDS             complement(2041..2562)
                     /locus_tag="PLV68_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HRE01488.1"
                     /translation="MTPLEQLWNGWRASYVTSLSPRHEGAPDTASVFSRILASGLPDA
                     ETHIVHRGEHTFAILNAFPYSVGHVLVLPYRETESLESLTAAETNELWATVTDTVRTL
                     KAVMRPGGVNVGLNLGEPAGGSVSQHLHVHVVPRWNGDANFMTSTANTRTLPEALPDT
                     AAKLRAGWVRGLA"
     gene            complement(2569..>3264)
                     /locus_tag="PLV68_09320"
     CDS             complement(2569..>3264)
                     /locus_tag="PLV68_09320"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HRE01489.1"
                     /translation="DFTANLSTRDPKKSVGSDEIWAKATDALRAALERHGLAYKIKHG
                     DAAFYGPKIDIDVRDAIGRSWQLSTIQCDFNHPERFELEYVGSDNARHRPIMLHRALF
                     GSIERFFGVLLEHFAGAFPAWLAPVQARILPVASSHEPYAHAQAEMLRAHGFRVDVVD
                     ANDQLGKRIRAAKLEKLPYVLVVGDDDVAATTFGVNPRGGEVERGVGAADFVARLAAE
                     VAARSLPVATTTE"
     assembly_gap    3265..3282
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3283..>4459)
                     /locus_tag="PLV68_09325"
     CDS             complement(<3283..>4459)
                     /locus_tag="PLV68_09325"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HRE01490.1"
                     /translation="AETDLGAPLPDGAAVAIITADSDEGRHVLRHSTSHVLAQAVTQL
                     FPGAKFSIGPAIADGFYYDFDLPGGATFSEDDLRAVEARMREIIKADQPFTRAEMGID
                     AAYELFADQPYKQEIIDRVRALSAAPSNPDDPDFDEDAATDTGEVAVGGTVSVYRNTP
                     EFVDMCRGPHVPSTAKLGHFKLMKVAGAYWRGNEKGPMLQRIYGTAWESKAALDEHLH
                     RLEEAERRDHRKLATELDLLSFPAELGGGLAVWHPKGGIIRKLMEDYSRERHVNGGYQ
                     FVYTPHLTRSTLYETSGHLHWYKDGMYPPMEMDNGTYYMKPMNCPMHCLIFASRQRSY
                     RELPLRLFELGTVYRYERAGTLHGLMRIRGFTQDDSHIYCTEDQLQDEIASLLDFVLS
                     "
BASE COUNT          674 a         1475 c         1434 g          687 t
ORIGIN      
        1 cgcgatcgaa gctcgtccgg ccgattcgtg gaccaccacc gaggtcgatc agctgcaaca
       61 gacgatcaac ggcgctgccc gcgcgatccg caccttggag gacgccagcg cggcggtcga
      121 cgactgatcg ccgctggtcg accccgactg tcgggccgcg atccggtcgc gtaggatgcc
      181 gacgttcggg ggtcggctgg gcaaacgccg attgnnnnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnatccc gatcccgacg acacgggcgg tctgcgctac
      421 ttcgacggac gggcctggag cgagcaccgc gcccgcctgc acctgccagc gccggcaccg
      481 aatcaaccca cggccgcgat gtgcgcctgt ggcgtcgtgg cgatcgggcg ttgccgggtg
      541 tgcgccgagg cgtactgcag cgctcacatc tcctcgacag cggtcgacga ccgacccttc
      601 cgcaagcggt gggaaccgtg gacctgcggc aactgcatcg aagacgcgca gcgcgagatc
      661 cgtgccctcc aactcgcccg ctgcgacaac gtgatgatgc agatggtgcg catcccccac
      721 ctcaaccggg tgaagacccc caacggtcgc cgcccgccgg tgatcagcct gttccaacac
      781 ccgcgcagcc ccaccgagcg tccgccccgc tacgcccagg cctacctggt cgaatacgac
      841 ggcggcccgc cgaacccgac cttcgaaggg ctcgccctgt cgatcgacgg ctcgaccgtg
      901 ttcaacatcg gccttccggt cgccggtgtc gctcgtcaac ggttcggccc caagcgcacg
      961 atcaccggat acatcgtcaa gaacgtgatc gacatcgacc ggctcagcga tgccgccgcc
     1021 cagcccacca ccgagtcgtg gttcgagttc gctgcccgtt cgtacctgcg gtgcgctcac
     1081 cacatcggta tcaagcccga cttctcgctg gtgttcgacc ctgcgccgct gcccgaactc
     1141 gaaccggcca gcgctgcgat tgccgatgcg gaaccccaca cggtgccagc gcgcgacatg
     1201 gatcacgacg agccggcacc cgctccgacc gcggcgaaca catcacggag caaacgaccc
     1261 aagaagccgc gcgccgctgc aaccaatccg atcgaccaac ccagcgatga gccgttgatc
     1321 agcgacgacg aatcgcgcgc ggatggcatc cctcggcccc acgtcggcga cgacggcatc
     1381 atcaacgacg tggcccatgt cgaggtaccc gcggccgatt cgtccccgtc gcaagagccc
     1441 gcggtgcgcc agcccgatcc tccggccagt cccccgctcc cgctccccga gccgtaacaa
     1501 gccggcgact ctctggccgg caggatcggc gaggcgaccg gctcagtcct cccagtcggt
     1561 ctcggggttg tcctgcacca ggtgctgcaa cacggtgccg tcgacggcgt cgaccagcac
     1621 cagaccgcgg cggcgaggcg ggttctcgct ggagtaacac agcacccgcc aggtggggcg
     1681 gctgcgtagc ccgcgccaca cctgctgggc cgacgcgtgg cccaccgcaa acccaactgc
     1741 cgaggtcgcc gcgaccagcg cctgcttttc gtcgacgtgc atccgccagc ccgacgtgag
     1801 gcagaccgcg ccgaacgcgg cgagcaagat cgccccgtac aacatgccgt cgttgatcaa
     1861 cacgggtgtg tcgttgcggg tgaccagcca ggcgaagaag ctgatcacgg cgatcgccag
     1921 gtacagcaca ccggggatgc gacggcggct gttgtcgggg aactggtagg ggccgacgaa
     1981 ctcgctggca tcgaggtctt cgggcagttc gtcgcggatc tcgtcggacg tcatgatggt
     2041 tcaggctagg ccgcgcaccc aaccggcacg cagtttcgcc gccgtgtcgg gcagcgcttc
     2101 gggcagggtg cgcgtgttgg cggtcgacgt catgaagttg gcgtcaccgt tccagcgtgg
     2161 cacgacgtgc acgtgcaggt gctggctcac gctgccgccc gcaggctcgc ccaggttgag
     2221 cccgacgttg acgccgccag ggcgcatcac cgccttgagc gtgcgcaccg tgtcggtgac
     2281 cgtggcccac agctcgttcg tctcggctgc ggtcaacgat tccaagctct cggtctcgcg
     2341 gtacggcagc accagcacgt gaccgacgct gtacgggaac gcgttgagga tcgcgaaggt
     2401 gtgctcgccg cgatggacga tgtgcgtctc ggcgtcgggt agccccgacg ccaggatgcg
     2461 actgaacacg ctggccgtgt caggcgcgcc ctcgtggcgc ggactcagcg acgtgacgta
     2521 gctggcccgc cacccattcc acagctgttc gagcggtgtc actcgcgatc actcggtcgt
     2581 ggtcgccacg ggcagcgagc gcgctgcgac ctcggccgcc aggcgggcga cgaagtcggc
     2641 cgcgcccaca ccgcgttcga cctcgccacc gcgtgggttg acgccgaagg tggtggcggc
     2701 cacgtcgtcg tcgccgacca ccagcacgta gggcagcttc tccaacttgg cggcacggat
     2761 gcgcttgccg agctgatcgt tggcatcgac gacgtcgacc cggaacccgt gggcacgcag
     2821 catctcggcc tgggcgtgag catacggctc gtggctcgac gcgaccggca ggatgcgggc
     2881 ctgcacgggc gccagccacg ccgggaacgc acccgcgaag tgctccaaca gcacaccgaa
     2941 gaaccgctcg atcgagccga acagggcgcg gtgcaacatg atcgggcgat ggcgggcatt
     3001 gtcgctgccg acgtattcca gctcgaaccg ctcggggtga ttgaagtcgc actggatcgt
     3061 gctcagctgc cagctgcgac cgatcgcgtc gcgcacgtcg atgtcgatct tgggaccgta
     3121 gaacgcggcg tcgccgtgct tgatcttgta cgccaggccg tggcgttcga gcgcggcgcg
     3181 cagcgcgtcg gtggccttgg cccagatctc gtcggaaccg accgacttct tcgggtcgcg
     3241 cgtgctgagg ttggccgtga agtcnnnnnn nnnnnnnnnn nngacaacac gaagtcgagc
     3301 agcgaggcga tctcgtcctg gagctggtct tcggtgcagt agatgtgact gtcgtcctgc
     3361 gtgaagccgc ggatgcgcat cagcccgtgc agcgttccgg cccgctcgta gcggtacacc
     3421 gtgcccagct cgaacagccg tagcggtagc tcgcggtacg agcgctggcg gctggcgaag
     3481 atcaggcagt gcatcgggca gttcatcggc ttcatgtagt acgtgccgtt gtccatctcc
     3541 atcggcgggt acatgccgtc cttgtaccaa tggaggtggc cgctcgtctc gtacagcgtc
     3601 gagcgggtga ggtgcggcgt gtacacgaac tggtagccgc cgttgacgtg gcgctcgcgg
     3661 ctgtagtcct ccatcagctt gcggatgatg ccgcccttgg ggtgccacac cgcgaggcca
     3721 ccgccgagtt cggccggaaa gctcagcaga tcgagctcgg tggccagctt gcggtggtcg
     3781 cgacgctcgg cttcttccag gcggtgcaag tgctcgtcga gcgcggcttt gctctcccac
     3841 gccgtgccgt agatgcgttg cagcatcggc cccttctcgt tgccgcgcca gtacgcgccg
     3901 gccaccttca tcagcttgaa gtggccgagc ttggccgtgc tgggtacgtg cgggccgcgg
     3961 cacatgtcga cgaactcggg cgtgttgcgg tacaccgaga cggtgccgcc gacggcgacc
     4021 tcgccggtgt cggtcgccgc gtcctcgtcg aagtcggggt cgtcgggatt gctgggcgcg
     4081 gccgaaagag cgcggacccg gtcgatgatc tcctgcttgt acggctggtc ggcgaacaac
     4141 tcgtaggcag cgtcgatgcc catctcggcg cgcgtgaacg gctggtcggc cttgatgatc
     4201 tcgcgcatcc gcgcttcgac cgcgcgcagg tcgtcttcgc tgaaggtcgc gccgcccggt
     4261 aggtcgaagt cgtagtagaa gccgtcggcg atcgccggac cgatgctgaa cttggcgccg
     4321 gggaagagtt gcgtcaccgc ttgggccagc acgtggctgg tcgagtggcg cagcacgtga
     4381 cgaccctcgt cgctgtcggc cgtgatgatc gccaccgccg cgccgtcggg cagcggcgcg
     4441 ccgaggtcgg tctcggcac
//
LOCUS       DAORNF010000207         4456 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_14565_length_4456_cov_1.697342, whole genome shotgun sequence.
ACCESSION   DAORNF010000207 DAORNF010000000
VERSION     DAORNF010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4456
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14565_length_4456_cov_1.697342"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            177..>669
                     /locus_tag="PLV68_09330"
     CDS             177..>669
                     /locus_tag="PLV68_09330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01491.1"
                     /translation="MDVDAFVVRHQEVWWRLAQLTNLARRPKSLHPEELDELVQLYQR
                     TSAHLAAARVTYAADPTLVGRLTLLVADAHAVLYGQRDTDVRRSAARFATDTFPQAVY
                     AIRWFILAATLLTLVPWAVMNVWLAVSPRAVNATGPATLRDQYINASFEEYYRSDSAT
                     AFAN"
     assembly_gap    670..872
                     /estimated_length=203
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <873..1202
                     /locus_tag="PLV68_09335"
     CDS             <873..1202
                     /locus_tag="PLV68_09335"
                     /inference="COORDINATES: protein motif:HMM:NF014052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HRE01492.1"
                     /translation="GLRIGWAVIDPGDRSRAEALREHGRTAGAVVMGLIAAFLLAALV
                     EAFVTGRAWPTALRLVIGIAAFTIFWSWTIVNAVYGRDDDTNRSHDDNPHDSDPDYRR
                     DEAFSSR"
     gene            complement(1169..2479)
                     /locus_tag="PLV68_09340"
     CDS             complement(1169..2479)
                     /locus_tag="PLV68_09340"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HRE01493.1"
                     /translation="MPIPTARFALVVALSAVVVLAVAGDDWSVLWLVEAVLLVVMLAD
                     AFAAVSPSSIDVARSHPESIVLGETTVVTWTIENRAERAVHVAVGDALWPSLNATRRR
                     VETVLHPLARHVASARLRPERRGRFPLDEVVVRTRGPLGLAVRHRVQHAPSVLRVMPA
                     FPSRDSIQQRLSRPRVIDVGVRALRVRGSGTEFDQLREMRLGDDPQRIDWAATAKHQR
                     PIVRQVRAEHDQSVVILLDNGRVMAGQIDGTPRVEHAMDAALGLTAVATHLGDRVGLV
                     SFDRSVVTVVPPSHAKTQPRLVAEAMYQLEPELSESAYLSAFSYATARFRRRSLYVVL
                     TELAEAAVEEAILPALPILCRHHLVIVATVQDPLVSEWAAGTGGTAASGMFRQAAAIA
                     QLQHRRRSVARLTAAGAIVIDARPGELATRLVDTYLELKASSRL"
     gene            complement(2483..>2780)
                     /locus_tag="PLV68_09345"
     CDS             complement(2483..>2780)
                     /locus_tag="PLV68_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRE01494.1"
                     /translation="AIRVEPNVLQYIVSLCRATRESPSLTLGVSPRGATWLLHATKAW
                     AWLSGGGFVTPDEVKAVAKPCLRHRIMVRPELELEGTIGDAVLDGILATVPTPR"
     assembly_gap    2781..2790
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2791..3543)
                     /locus_tag="PLV68_09350"
     CDS             complement(<2791..3543)
                     /locus_tag="PLV68_09350"
                     /inference="COORDINATES: protein motif:HMM:NF040105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRE01495.1"
                     /translation="MTNETNEMNETNAVTATSAAPRPSALDAIRAVQGEVDKVVVGQA
                     GIISGVITALLVNGHVLLEGVPGVAKTLLAKSIAASFDMTFNRVQFTPDLMPADVIGQ
                     SIYEMRPGESAGFRFREGPVFTNLLLADEINRTPPKTQAALLEAMEERQVSIDGQTRK
                     LPDPFLVIATQNPIEQEGTYPLPEAQLDRFMLSVKVSYPTEDEEVEIVERTTTGREVE
                     VKPVLDRELLIQLQGLVRRLPTSKEVHRYCVRL"
     gene            complement(3540..>4456)
                     /locus_tag="PLV68_09355"
     CDS             complement(3540..>4456)
                     /locus_tag="PLV68_09355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01496.1"
                     /translation="AVVADPLSPLHGGQQAGASELAVAAPGGIFGTGVRLSAEDEANV
                     DRGVCSIEALAHLRGIFTPRGVEFRVQDGQPNCFGDGRLAFVIVRSVGRGVVVGLGDN
                     RPLTNAYLRYADNAGLVTALVAPQARMQVRIMIGSEARPTAADVGSGDERLADLVEPS
                     VWMALTQLALAFAVLAVARGWRAGASIAEPQRVPIAGSELVTATANVMQRARRGERAA
                     WLLRAELYRNLADRYRCPPDVSIDGLVSAVVAHRPDCDARDLHAVLTWPTSATADDLV
                     QLGNRIEALRVQLLTEPATPTPTREVVP"
BASE COUNT          663 a         1496 c         1463 g          621 t
ORIGIN      
        1 gcgtcgatcg ctcccgacac caaccgcgat ccgaaacctg ccgtcgccag ctccagcacc
       61 accgcctccg gcgtgacgat tccctcgacc cgctcccccg cgccagccat gtcccccacg
      121 agcgccatcg ccgccaccct agcgagtcgg cccgttgggc cagtacggtc ggttcagtgg
      181 atgtcgacgc gttcgtggtt cgccaccagg aggtgtggtg gcggctggct cagctgacga
      241 acctggcacg gcgaccgaag tcgttgcatc cggaagagct cgacgaattg gtccagctct
      301 atcaacgcac cagcgcccac ctggccgcgg ctcgggtgac ctacgcggcc gatcccacgc
      361 tggtcgggcg gctcacactg ctggtcgccg acgcgcacgc cgtgctgtac ggccagcgcg
      421 acaccgacgt gcgccggagc gcggcccggt tcgcgaccga cacgttcccc caggcggtgt
      481 atgcgatccg ctggttcatc ctggccgcca cgttgctgac gttggtgccg tgggcggtga
      541 tgaacgtgtg gctcgcggtg tcgccgcgag cggtcaacgc gaccgggcca gcgacgctgc
      601 gcgaccagta catcaacgcc agcttcgagg agtactaccg gtcggattcg gcaacggcgt
      661 tcgccaaccn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngggctgcg gatcgggtgg gcggtgatcg
      901 accccggaga tcgctcacgg gccgaggcgc tgcgcgaaca cgggcgcaca gccggcgccg
      961 tggtgatggg gctgatcgcg gcgttcctgc tggcggcgct ggtcgaagcg ttcgtcaccg
     1021 ggcgagcgtg gccgacggcg ttgcgtctgg tgatcggcat cgccgcgttc accatcttct
     1081 ggtcgtggac gatcgtcaac gcggtctacg gacgggatga cgacaccaac cgatcacacg
     1141 acgacaaccc gcacgacagc gacccggact acaggcggga cgaggccttc agttcgaggt
     1201 aggtgtcgac caagcgcgtc gccaactcgc caggccgggc atcgatcacg atcgcgcccg
     1261 cggccgtcag acgggccacg ctgcggcggc ggtgctgcaa ctgggcgatc gccgcggcct
     1321 ggcggaacat gccgctggcg gccgtgccac cggtaccggc ggcccattcg ctgaccagcg
     1381 ggtcctgcac ggtggcgacg atcaccaagt ggtgacggca caggatcggc aaggcgggca
     1441 ggatcgcttc ttcgaccgcg gcctcggcca actcggtcag caccacatac aacgagcggc
     1501 gccggaaccg cgccgtggcg tagctgaacg cgctgaggta ggcactctcg ctgagctcgg
     1561 gttccaactg gtacatcgcc tcggccacca ggcgcggctg ggtcttggca tggctgggcg
     1621 gcaccacggt gacgacgctg cgatcgaagc tgaccaggcc cacccggtcg ccgagatggg
     1681 tcgccaccgc cgtcagcccg agcgcggcat ccatcgcatg ctcgacgcgc ggcgtgccgt
     1741 cgatctggcc ggccatgacg cggccgttgt cgagcaggat cacgacgctc tggtcgtgct
     1801 cggcccgcac ctggcgcacg atcggccgct ggtgcttggc ggtcgcggcc cagtcgatgc
     1861 gctgagggtc atcacccaga cgcatctcgc ggagctgatc gaactcggta ccgctgccgc
     1921 ggacgcggag cgcgcgcacg ccgacgtcga tcacccgcgg acggctgagg cgctgctgga
     1981 tgctgtcccg gctgggaaac gcgggcatca cccgcaacac gctcggagcg tgctgcaccc
     2041 ggtggcgtac ggcgaggccg agcggaccac gggtgcgcac caccacttcg tcgagcggga
     2101 accggccgcg ccgctcggga cgcaagcgtg ccgacgcgac atgacgagcg agggggtgca
     2161 acaccgtctc gacgcggcgc cgggtggcgt tgagactcgg ccacagcgca tcaccgacgg
     2221 caacgtgcac cgcgcgttcg gcgcggttct cgatcgtcca cgtcacgaca gttgtttcac
     2281 ccagaacgat cgactcggga tgcgagcggg cgacatcgat cgacgatggc gagaccgcgg
     2341 cgaaggcgtc ggccagcatc accaccaaca acaccgcttc gaccaaccac aacaccgacc
     2401 agtcgtcacc ggccaccgcc aacaccacga ccgcgctgag cgcgaccacg agagcgaagc
     2461 gggcggtggg gatcggcatg ggctatcgcg gcgtgggcac ggtggcgagg atgccgtcga
     2521 gcacggcgtc gccgatggtg ccctccaact ccaactcggg gcggaccatg atgcggtggc
     2581 gcaggcacgg cttggccacg gccttcacct cgtcgggggt cacgaagccc ccgccgctca
     2641 accaggccca cgccttggtg gcgtgcaaca gccaggtggc tccacggggc gacacgccca
     2701 gcgtcagcga cggcgattcc cgcgtcgccc ggcacagcga gacgatgtac tgcaacacgt
     2761 tgggctcgac ccggatggcg nnnnnnnnnn gaggcgcaca cagtaacgat gcacctcctt
     2821 gctggtcggc aggcggcgca ccaggccctg gagctggatc agcagctccc ggtccaggac
     2881 cggcttgacc tcgacctcgc gcccggtggt ggtgcgctcg acgatctcga cctcctcgtc
     2941 ctcggtcggg tagctgacct tcaccgagag catgaagcgg tcgagctggg cctcgggcag
     3001 cgggtaggtg ccctcctgct cgatcgggtt ctgcgtggcg atcaccagaa acgggtcggg
     3061 cagcttgcga gtctgaccgt cgatgctgac ctggcgctct tccatcgcct ccaacagtgc
     3121 ggcctgggtc ttgggtggcg tgcggttgat ctcgtcggcc aacaacaggt tggtgaacac
     3181 cggcccctcg cggaagcgga accctgccga ctcgccagga cgcatctcgt agatgctctg
     3241 gccgatgacg tcggccggca tcaggtccgg cgtgaactgc acgcggttga acgtcatgtc
     3301 gaagctggcc gcgatcgact tggccagcag tgtcttggcc acgccgggta ccccttcgag
     3361 cagcacgtgg ccgttgacca acagcgcggt gatgacgccg ctgatgatgc cggcctggcc
     3421 gacgaccacc ttgtcgacct cgccctgcac ggcgcggatc gcgtcgagcg cgctcggccg
     3481 cggcgcagcg ctcgtcgcgg tcacagcgtt cgtctcgttc atctcgttcg tctcgttggt
     3541 cacggaacca cctctcgcgt cggggttggg gttgcgggtt cggtcagcag ttgcactcgc
     3601 agcgcctcga tccggttgcc gagttgcacc aggtcgtcgg ccgtcgcgct ggtgggccac
     3661 gtcagcaccg cgtggaggtc gcgagcgtcg caatcgggtc ggtgcgcgac cacggccgac
     3721 accagcccat cgatcgagac gtcgggcggg cagcgatagc gatcggccag gttgcggtag
     3781 agctcggcgc gcagcaacca cgcggcccgc tcgccgcgcc gggcgcgctg catcacgttg
     3841 gctgtcgctg tcaccaactc gctccccgcg atcggcaccc gctgcggttc ggcgatgctg
     3901 gccccggcac gccacccgcg tgcgacagcc agtaccgcga acgccagagc cagctgcgtc
     3961 aacgccatcc acacgctggg ctcgaccaga tcggccaggc gctcgtcgcc cgagccgaca
     4021 tcggcggccg ttggccgggc ctcgctaccg atcatgatcc gcacctgcat gcgcgcctgc
     4081 ggcgcgacca gcgcggtgac caacccggcg ttgtccgcgt agcggaggta ggcgttggtc
     4141 aacgggcgat tgtcgcccag cccgacgacg acgccgcgac cgacgctgcg cacgatcacg
     4201 aacgcgaggc gtccgtcgcc gaagcagttg ggctggccgt cctggacccg gaactcgacg
     4261 ccgcgcggcg tgaaaatgcc gcgcagatga gcgagcgcct cgatgctgca gacgccgcgg
     4321 tcgacgttgg cttcgtcctc ggccgacaag cgcacgccgg taccgaagat gccgcccggg
     4381 gccgccacgg ccaactcgct cgcaccggcc tgctggccgc cgtgcagcgg gctgagcggg
     4441 tcggcgacca cggcga
//
LOCUS       DAORNF010000208         4456 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_14566_length_4456_cov_1.514883, whole genome shotgun sequence.
ACCESSION   DAORNF010000208 DAORNF010000000
VERSION     DAORNF010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4456
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14566_length_4456_cov_1.514883"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            111..443
                     /locus_tag="PLV68_09360"
     CDS             111..443
                     /locus_tag="PLV68_09360"
                     /inference="COORDINATES: protein motif:HMM:NF024804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HRE01497.1"
                     /translation="MCGFVLRTGVQFPHDCNFTIGKSFMNLLSTHGIVLTAIARQPDI
                     RLRELAINLDVTERTVWRAVEDLVLAGVVVRHKVGRRNSYTVNGKVDVGDHTGSVNVA
                     ALLKAVNS"
     gene            complement(433..1035)
                     /locus_tag="PLV68_09365"
     CDS             complement(433..1035)
                     /locus_tag="PLV68_09365"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRE01498.1"
                     /translation="MLILLRHGRTALNAAGVLQGRVDQPLDEVGRAQASQVAAALGPA
                     DLVITSSLQRARETAGYFGRETVVDDRWIEIDYGRMEGQPVSVLSSPEWVRVRMDPAV
                     PFDGGESLAAVAARVDPAFADALELARDRTVVVVSHVSPIKAAIGVTLGCDHPIGFRC
                     HLDHASVSRVESRSTGPVLRSFNEVLYHISPEPRPEVVRS"
     assembly_gap    1208..1328
                     /estimated_length=121
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1329..1559)
                     /locus_tag="PLV68_09370"
     CDS             complement(<1329..1559)
                     /locus_tag="PLV68_09370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01499.1"
                     /translation="MLFWFLGTAVAAVWFVFRDPRFDYRLLCIGALVPDAIDVWFGGA
                     RAMHSITISIAVLAVIVAASAGRRRWRKPALAV"
     gene            complement(1564..2271)
                     /locus_tag="PLV68_09375"
     CDS             complement(1564..2271)
                     /locus_tag="PLV68_09375"
                     /inference="COORDINATES: protein motif:HMM:NF014630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HRE01500.1"
                     /translation="MTTPETLLAIQRLDSAIDLARLRLPKLDAVVASAQADAALRAWQ
                     QRTDALSAEIAGHEQAITAAEHATADLTKHLTRLDQQLKSVIVAREADALMAEIATLK
                     AKRGEYDDQELAALDAIAEAETALGAHRSDYDGLRASAEHARTAADQARVESEADMAA
                     KATERDALRAGLAADVLAMYDTMRGQHQGIAVAQLSGLQCGGCHLDLSRAEVDEIKQL
                     DGNALPECPNCGRILVR"
     gene            complement(2375..>3058)
                     /locus_tag="PLV68_09380"
     CDS             complement(2375..>3058)
                     /locus_tag="PLV68_09380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01501.1"
                     /translation="DPGTQVLDQGAALTTVSMLKGVFSGTGHRYPLADGRPAAGKTGT
                     QDNNTNAWFVGMTPELSTAVWVGNPNGYVEMNGIPEFVADGVSRVQGGYYPEKIWKTF
                     VDAALAGTPHQDWPAAPATTRPAMRLYLPGEECLGKLVSGVLPGAPTTPTTPPVPELD
                     PETGQPVETTTTTAPAPVIQAIDSGTTIAPDVVDPNAPLNTVDTKTLVFNCERGLASV
                     VTTTTAAGG"
     assembly_gap    3059..3161
                     /estimated_length=103
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3162..3366)
                     /locus_tag="PLV68_09385"
     CDS             complement(<3162..3366)
                     /locus_tag="PLV68_09385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01502.1"
                     /translation="MTASSINCAFARLSQIVGLNRVVDTTYRMAHSDYLYAGQTREQR
                     GGVDPIQPFASYATGANEMSPMDM"
     assembly_gap    3401..3508
                     /estimated_length=108
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3509..>4456)
                     /locus_tag="PLV68_09390"
     CDS             complement(<3509..>4456)
                     /locus_tag="PLV68_09390"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HRE01503.1"
                     /translation="IAIFEQENVQPFTLDQVPEVVVQAVLSVEDNEFYVHDGVNVRGM
                     FRATLSNFSAGANQQGASTITQQVVKNEYLAGLPRDGRYKLLQVHYALMLEKEKTKDE
                     ILERYLNTIFFGNNAYGLAAAAEIYFGETVEQLTLPQGAFLAGLIRSPSGYDPFRRPE
                     RARARFEQVLDRLVATEMVTDDEATTLLETWPIPEIPQVVPGRSTVPTYFTVALQDYL
                     LNVSDILGKDEQERRSRMVRGGLRIHTTLDPAMQAAAEAARDVLPDTIQGFDAAIVSL
                     DSTSGAIRVMVGGRGFKRNINEINMALRPRQTGSSIKLFI"
BASE COUNT          631 a         1399 c         1374 g          720 t
ORIGIN      
        1 cgacgactcg gcaaccctcg ggtgaaccgc acagatggat ggtcatcgtg ccgctcgtcg
       61 gcatacagaa tcccgcttac aggtcgactc accagcaaag tttgcgccag gtgtgcggat
      121 tcgtgctaag aacaggggtg cagtttcccc acgactgcaa cttcacgatc ggaaagtcct
      181 tcatgaacct gttgagcacc cacggcatcg ttctcacggc catcgcccgt cagcccgaca
      241 ttcgactgcg tgaactggct atcaacctcg atgtcaccga acgcaccgtg tggcgcgctg
      301 tcgaagatct ggtgttggct ggcgttgtcg tccggcacaa ggtcggccgc cgcaactcct
      361 acaccgtcaa cggcaaggtc gacgtcggtg accacaccgg cagcgtcaac gtcgctgcac
      421 tgttgaaggc cgtcaactcc tgacgacctc cggccgcggc tccggcgaga tgtggtagag
      481 cacttcgttg aagctgcgca acaccggacc cgtcgatcgc gattccactc gcgacaccga
      541 cgcatggtcg aggtgacaac ggaagccgat ggggtggtcg cagcccagcg tgaccccgat
      601 tgcagccttg atcggcgaca cgtggctcac gacgaccacg gttcgatcgc gcgccaactc
      661 gagcgcgtcg gcgaacgccg ggtcgacccg cgctgccacg gccgcgagtg actcaccgcc
      721 gtcgaagggc actgccggat ccatacggac gcgcacccac tcggggctcg acagcaccga
      781 gaccggctgc ccctccatcc ggccgtagtc gatctcgatc caccggtcgt cgaccaccgt
      841 ctcacgaccg aagtacccag cggtctcccg tgcccgctgc agcgaactgg tgatgacgag
      901 gtcggctggt ccgagcgccg cagccacctg actggcctgt gcccggccca cctcgtcgag
      961 gggttgatcg acccggcctt gcagcacccc cgctgcattc agcgcggtgc gtccgtgacg
     1021 aagcaggatc aacacgtgcc cagtgaagca caccgcaccg cgtcagcgac gatgcggtgg
     1081 ctgtgccggc gcgaccgggt gcagttgacc ggtgcgccat aggtcgcgtc ggcggaccgg
     1141 atcggtgaga ccgaaggtgc gcacggccca ccacaacagg ccgagtccgg ccagttcgag
     1201 cgggatgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnca ccgccagggc cggcttgcgc caccgtcggc gcccggcgct ggcagcgacg
     1381 atcacggcga gcaccgcgat gctgatcgtg atgctgtgca tcgcccgcgc tcccccgaac
     1441 cacacgtcga tcgcatcggg cacgagtgct ccgatgcaga gcaggcggta gtcgaagcga
     1501 ggatcgcgga agacgaacca caccgccgcc acggcggttc cgagaaacca gaacagcacg
     1561 gtgtcaacga accaggatgc gcccgcagtt cgggcattcc ggcagggcgt tgccgtccaa
     1621 ctgcttgatc tcgtcgacct cggcccgcga caggtcgagg tggcagccgc cgcactgcag
     1681 gccgctgagt tgcgcgacgg cgatgccttg gtgctggccg cgcatcgtgt cgtacatcgc
     1741 caggacgtcg gcagcaagtc cggcgcgcag tgcatcgcgc tcggtggcct tcgcagccat
     1801 gtcggcctcg ctctcgaccc gggcctggtc ggccgcagtg cgggcgtgct cggcgctcgc
     1861 gcgcagcccg tcgtagtcgc tgcggtgtgc gcccagcgca gtctccgctt cggcgatcgc
     1921 gtcgagcgca gcgagctcct ggtcgtcgta ctcgccgcgc ttggccttca acgtggcgat
     1981 ctcggccatc agcgcgtcgg cttcgcgggc gacgatcacc gacttgagct gttgatcgag
     2041 gcgagtgagg tgcttggtca ggtcggccgt ggcgtgttcg gccgcggtga tcgcctgctc
     2101 gtgcccggcg atctcagcgc tgagcgcgtc ggtgcgttgc tgccaggcgc gcaaggcggc
     2161 gtcagcctgc gcgctggcga ccaccgcatc gagcttgggc aggcgcagcc gagcaagatc
     2221 gatggcgctg tcgaggcgtt ggatcgcgag cagcgtctct ggtgtcgtca tggctctttc
     2281 ctatcatccg cccgcgacgg tgctgcctat ccgcctgccg cggttgctcc tctgcgcctg
     2341 cgccgttgtc ctcatccgcc tgcggctgct gtcctcatcc gcctgcggct gttgtggtcg
     2401 tgaccaccga cgccagtccg cgttcgcagt tgaacaccaa cgtcttggtg tcgacggtgt
     2461 tgagcggggc gttggggtcg accacgtcgg gcgcgatcgt ggtgccgctg tcgatcgcct
     2521 ggatcacagg ggcaggcgcg gtcgtggtgg tggtctcgac cggctggccc gtctcggggt
     2581 cgagttcggg caccggcggt gtggtcggcg tcgtcggcgc accgggcagc acaccgctga
     2641 cgagcttgcc gaggcactcc tcccccggca ggtacaaccg catcgccgga cgggtcgtgg
     2701 cgggcgctgc tggccagtcc tgatgcgggg tgccggccag ggccgcgtcg acgaacgtct
     2761 tccagatctt ctccgggtag tagccgccct gcacccgcga caccccgtcg gcgacgaact
     2821 cggggatgcc gttcatctcg acgtagccgt tggggttgcc gacccagacg gcggtcgaca
     2881 gctcgggggt catgcccacg aaccaggcgt tggtgttgtt gtcctgtgta ccggtcttgc
     2941 ccgccgcggg ccgtccgtcg gccagtggat agcggtgtcc ggtcccgctg aacacgccct
     3001 tcagcatcga cacggtcgtc agcgccgcac cctggtcgag cacctgcgtg cccgggtcnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nccatgtcca tcgggctcat
     3181 ctcgttcgcc ccggtggcat agctggcaaa cggctggatc gggtcgaccc caccgcgctg
     3241 ctccctggtc tggcctgcat acagatagtc ggagtgggcc atgcggtagg tggtgtcgac
     3301 cacgcggttg agcccgacga tctgcgacag gcgggcgaac gcgcagttga tcgagctggc
     3361 cgtcatcgac gccaaggtgt tcaacggctt gctgacgccc nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnga tgaacagctt gatgctggag ccggtctggc
     3541 ggggccgcag cgccatgttg atctcgttga tgttgcgctt gaaaccgcgc ccaccgacca
     3601 tcacccgaat cgcgccggag gtgctgtcga gcgacacgat cgctgcgtcg aacccttgga
     3661 tcgtgtcggg cagcacgtcg cgagctgcct cggccgcggc ctgcatcgcc gggtcgagcg
     3721 tcgtgtggat gcgcaacccg ccgcgcacca tgcggctccg ccgctcctgc tcgtccttgc
     3781 ccaggatgtc gctgacgttg agcaggtagt cctgcagagc gaccgtgaag taggtcggca
     3841 cggtgctgcg cccaggcacg acctgtggga tctcggggat cggccaggtc tccagcagcg
     3901 tcgtcgcctc gtcgtcggtc accatctcgg tcgcgacgag ccggtcgagc acctgctcga
     3961 accgcgctct ggcccgctcg ggacggcgga atgggtcgta cccggacggc gagcggatca
     4021 ggccggccag aaacgcgccc tgcggcaacg tgagctgctc gaccgtctca ccgaagtaga
     4081 tctccgccgc ggccgcgagc ccgtaggcgt tgttgccgaa gaagatcgtg ttgaggtagc
     4141 gctcgagaat ctcgtccttg gtcttctcct tctccagcat cagcgcgtag tgcacctgca
     4201 acagcttgta acggccgtcg cggggcagac cggcgaggta ctcgttcttg acgacctgct
     4261 gtgtgatcgt cgacgcgccc tgttggttgg caccggcgga gaagttcgac agcgtcgccc
     4321 ggaacatgcc gcgcacgttg acgccgtcgt gcacgtagaa ctcgttgtcc tccaccgaca
     4381 agacggcctg aaccaccacc tcgggtacct gatccagcgt gaacggctgc acgttctcct
     4441 gttcgaagat cgcgat
//
LOCUS       DAORNF010000209         4453 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_14580_length_4453_cov_1.834243, whole genome shotgun sequence.
ACCESSION   DAORNF010000209 DAORNF010000000
VERSION     DAORNF010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4453
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14580_length_4453_cov_1.834243"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            68..>2091
                     /locus_tag="PLV68_09395"
     CDS             68..>2091
                     /locus_tag="PLV68_09395"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HRE01504.1"
                     /translation="MFRGLRHHHDIEDQLVVPIVAERTDDDERHHLDVLHAQHDAIVT
                     LVEQADAQLAVWQLDTAHANRDQLAALLERIDELLVEHLDTEERFVFPIAERLLTPAE
                     WDEIGRRAEAGYRLRERILSFGMIQHEGEPEVLASMLATAPTLVRRLLPVFARRAYRR
                     HSHQIHGGPSATARTRTNGWRIALALGGALMAAGGPLHPESDAKDPLREELATMTAGD
                     TWVLSHSLIAAGTVLLALGLWSAVRSSRWPQSMMRALRVTAVAMSLYVVETIFHLAAV
                     LDSRALADGDAAPIAFTHIGLSVLLYPVSGLAFAWLNTHLLRCRSFRDVLGGVVGVAS
                     GLLQAAAVPLTLALPDTEFTPVFASGSMLLATWSLLVGVFGLRSRGTTGSKTTTALAV
                     ASLAAVVSVTAVVTELAPSPTEAATAYPPGAGMRGYRIYDLSGGAINFELMTTTGLSD
                     NGVVSGNWVGHDNSVHPFFWKPGVGWQNLDRGSAINAWANAISPDGSTIVGYDYDPAT
                     PAPHQHRVEWTWDDELGIYGAAQRVEPFGHETADVPLAVNDDGDVLVAGAVIDSDGGR
                     TPLALPPYTPEQRPTYTAHAMNANGDVTGLVSYDPDGDLGPLSLQIVPAVWTAGTPTE
                     LGLPPGAAAAWGYAINDSGDVAVGFGGGNVSWLREGNGWVEMAPFAPSDI"
     assembly_gap    2092..2120
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2374..2495
                     /estimated_length=122
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2496..3913
                     /locus_tag="PLV68_09400"
     CDS             <2496..3913
                     /locus_tag="PLV68_09400"
                     /inference="COORDINATES: protein motif:HMM:NF014503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01505.1"
                     /translation="TGRSVRDLDIYGTFLEALVTQGGYVEYDVDDVISRRTAAGCDVS
                     QRDARPNLFVFAYDWRQDNAVSAVELADYIECVRRFHPSGAINVVTHSMGSLIARRYV
                     LDHPEADIGRMITVGAPWLGAPKLVYTLQTGVFAPPIASDPIIARLAQRFTATHQLMA
                     AELYHVATGQPVFIEDGWDINGNDVTIEQYSYEQLAMFLDGQSPVSDPAANATAFHSY
                     ATPRGAQVDWRDDQTGIEYFHVTGLQMANNTIGQVVARSTPSCLIGTTWFCSNDEHFV
                     INRAWGDGTVPLVSATRHGANGFDFNAPGATVLTAASGSVAEDFYSEHTGLAANPHVI
                     GTIIDLLAGGTLATTDDGGDANANAAAGITAGSTPTIAGAPIQTSLLVGPEAAAQAAA
                     DNATSPLRTITVLGATDVHVADGSGNSTRPPAGIDSDIRFSVPGVSDTILGDDAWLLT
                     LPATVLEQYAVTFTATRCGWN"
     gene            3904..>4453
                     /locus_tag="PLV68_09405"
     CDS             3904..>4453
                     /locus_tag="PLV68_09405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01506.1"
                     /translation="MELTSGDATQTSIATRWTDVDLADGTAVALVLGTDGIDRLRADL
                     DGDGTPETIIAPNVVVDGAIAADTDAPEVSIVATAPTNTNANTQYAVVATNTESGVAR
                     TLWSTDGESFALYDGPLALDASATPTIWAFAEDHAGNRSPMRQLRLAGAPLVPFTTTS
                     VSPAPNPGGWSRGTVSVEMHAAA"
BASE COUNT          689 a         1554 c         1386 g          673 t
ORIGIN      
        1 gttggtccgc ggcgtcgcgg acggcgatgc cgcgcgtgcc cagacggtcg cccggcacgt
       61 ggagttcatg ttccgcggcc tgcggcatca ccacgacatc gaagaccagt tggttgtgcc
      121 catcgtcgcc gaacggacgg acgacgacga acgccaccat ctcgacgtgc tccacgcgca
      181 gcacgacgcg atcgtcacgc tggtcgagca agccgacgcg caactcgcgg tctggcaact
      241 cgacactgcc cacgccaacc gcgaccagct cgccgcactg ttggagcgga tcgacgaact
      301 gctggtcgaa catctcgaca ccgaggagcg gttcgtgttt cccatcgccg aacgacttct
      361 cactccggcg gagtgggacg agatcgggcg ccgcgccgag gccgggtatc gactccgcga
      421 gcgaatactt tcgttcggaa tgatccagca cgaaggcgaa cccgaggtac tcgcatcgat
      481 gctcgccacc gcacccacgc tggttcggcg gctgctgccg gtgttcgctc gccgcgccta
      541 tcggcgccac tcgcaccaga tccacggtgg cccctctgcc accgcgcgca cacgcacgaa
      601 cggctggcgc atcgctctcg cccttggcgg tgcactgatg gcggccggcg ggccgctgca
      661 cccggagtcc gacgccaagg acccgctgcg cgaggagctc gccaccatga cggcaggtga
      721 cacgtgggtg ttgtcgcaca gcctgatcgc cgccggcacg gtgctgctcg cgctcggact
      781 gtggtcggcc gtgcgctcca gcaggtggcc gcagtcgatg atgcgggccc tgcgggtcac
      841 cgccgtcgcc atgtcgctgt acgtggtcga gacgatcttc caccttgcgg cggtactcga
      901 ctcgcgggcg ctcgccgacg gcgatgcggc tccgatcgcg ttcacccaca tcggtctgtc
      961 tgtcctgctg tacccggtga gcggtctcgc tttcgcctgg ctcaacacgc atctgctgcg
     1021 ctgtcgctcg ttccgcgacg tgctcggtgg ggtcgtcggt gtggcgtccg ggctgctcca
     1081 agccgcagcg gtgccactca ccctcgcctt gcccgacacc gagttcacgc cggtcttcgc
     1141 cagtgggtcg atgctgctgg cgacgtggtc gctgctggtc ggcgtcttcg ggttgcgttc
     1201 acgcggcacg acgggatcga agaccaccac cgcccttgcc gtcgcctcgc tcgcggccgt
     1261 cgtctcggtc accgcagtgg tcaccgagtt ggctccgtca cccaccgaag ccgccactgc
     1321 gtacccgccg ggagccggta tgcgcggcta ccggatctac gacctgtccg gtggcgccat
     1381 caacttcgag ctcatgacca ccaccgggct cagcgacaac ggtgtcgtgt caggaaactg
     1441 ggtcggccac gacaactcgg tccatccctt cttctggaag cccggcgtcg gttggcagaa
     1501 cctggatcgc ggcagcgcga tcaacgcctg ggccaacgcg atttcgcccg acggcagcac
     1561 gatcgtcggc tacgactacg accccgcgac tcccgcgccg caccagcatc gcgtcgagtg
     1621 gacctgggac gacgagctcg gtatctacgg cgcggcgcag cgagtcgaac cgttcgggca
     1681 cgagacagcc gacgtgccat tggcggtcaa cgacgacggc gacgttctgg tggcgggagc
     1741 ggtgatcgac agcgatggtg gacgcacccc tctggcgctg ccgccgtaca ccccggaaca
     1801 gcggccgacg tataccgccc acgcaatgaa cgcgaacggc gacgtcaccg ggttggtgtc
     1861 gtacgaccct gacggcgacc tcggcccgtt gtcgttgcag atcgtccccg ccgtttggac
     1921 ggccgggacg ccgaccgagc tcggattgcc gcccggtgcg gcggccgcgt ggggctatgc
     1981 gatcaacgac agcggcgacg tcgcggtggg gttcggtggc ggcaacgtgt cctggctgcg
     2041 cgaaggcaac ggctgggtcg agatggctcc gttcgctccg agcgacatca annnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn gcggtggtgt gaaacccgca cgccgatgct ggcgcgcaac
     2161 tcgtccacct tgacgacctg ctgctcgaca actccggctg ggagctgcgc gaggcagtca
     2221 cgatcaacga gtccgggcag atcgccggca tcggtatcaa ggacggcaag caggtgtcgt
     2281 tcctgctcga cccgatcacc ccagtggtct tcgtgcccgg ggccgcagcc agtcggctcg
     2341 tggtcagcga cgaggccgac gaagccgaag gtgnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnccacg ggacggtcgg tccgcgacct
     2521 cgacatctac ggcacgtttc tcgaagcgtt ggtcacccag ggcggttacg tcgaatacga
     2581 cgtcgacgac gtgatcagcc gccgcaccgc cgcgggttgc gacgtgtccc aacgcgacgc
     2641 gcgccccaac ctgttcgtct tcgcctacga ctggcgtcag gacaatgccg tctcggccgt
     2701 cgaactcgcc gactacatcg agtgcgtccg tcgctttcac ccgtcggggg cgatcaacgt
     2761 ggtcacccac tcgatgggca gcttgatcgc ccgccgctac gtgctcgatc atcccgaggc
     2821 cgacatcggc cggatgatca ccgtcggcgc gccctggctc ggtgccccca agttggtgta
     2881 cacgttgcag accggcgtgt tcgcgccgcc gatcgcctcc gacccgatca ttgcccggct
     2941 cgcacagcgc ttcaccgcca cccaccaact gatggccgcc gagctgtacc acgtggcgac
     3001 cggccaaccg gtcttcatcg aggacggctg ggacatcaac ggcaacgacg tcaccatcga
     3061 gcagtacagc tacgagcagc tcgccatgtt cctcgacggt cagtcgccgg tgagcgaccc
     3121 agcggccaac gccaccgcct tccacagcta cgcaacgccg cgtggtgccc aggtcgactg
     3181 gcgcgacgac caaaccggca tcgagtactt ccacgtcacc ggcctgcaga tggccaacaa
     3241 cacgatcggt caggtcgtcg ccaggtcgac tccgagttgc ctgatcggca cgacgtggtt
     3301 ctgctccaac gacgagcatt tcgtgatcaa ccgtgcctgg ggcgacggca cggtgccgtt
     3361 ggtgagcgcc acccggcacg gcgcgaacgg cttcgacttc aacgcccccg gagccaccgt
     3421 cctcacggcg gcatcgggca gcgtggcaga ggacttctac tccgagcaca ccggcctggc
     3481 cgccaacccg catgtgatcg gcaccatcat cgacctgctc gctggcggca cgctcgccac
     3541 cactgacgac gggggcgacg ccaacgccaa tgccgctgcg ggtatcacgg cgggcagtac
     3601 gcccaccatc gccggcgcgc cgattcagac ctcgctgctg gtcggccccg aggccgcagc
     3661 gcaagccgcg gccgacaacg ccacgtcacc gctgcgcacg atcaccgtgc tcggggcgac
     3721 cgacgtgcac gtcgctgacg gttcggggaa cagcacccgc ccaccggcag gcatcgactc
     3781 cgacatccgc ttctcggtac ccggtgtgag cgacacgatt ctcggcgacg acgcgtggct
     3841 gctgacgctg cccgcaacgg tgctggagca gtatgcggtc acgttcacgg ccacccggtg
     3901 cgggtggaac tgacgtcggg cgacgccaca cagacctcga tcgccacccg ctggaccgac
     3961 gtcgatctgg cagacggcac cgcggtggcg ttggtgttgg gcaccgatgg catcgaccgc
     4021 ctccgcgccg atctcgacgg cgacggcaca cccgagacga tcattgcgcc caacgtggtc
     4081 gtcgacgggg ccatcgcggc cgacaccgac gcccctgagg tctcgatcgt cgccaccgcg
     4141 cccaccaaca ccaacgccaa cacgcagtac gcggtggtcg ccaccaacac cgagtcgggc
     4201 gtggcccgca cgctgtggag caccgacggc gagtcgttcg cgctgtacga cggaccgctc
     4261 gcgctcgacg cgtcggcaac gcccacgatc tgggccttcg ccgaagatca cgccggcaac
     4321 cgctcgccga tgcgccagct tcgacttgcc ggagcgccgt tggtgccgtt cacgacgacc
     4381 tcggtcagcc ctgctcccaa cccaggcggt tggtcgcggg gcacggtctc ggtcgagatg
     4441 cacgcggccg ccg
//
LOCUS       DAORNF010000210         4426 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_14713_length_4426_cov_2.120567, whole genome shotgun sequence.
ACCESSION   DAORNF010000210 DAORNF010000000
VERSION     DAORNF010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4426
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14713_length_4426_cov_2.120567"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="PLV68_09410"
     CDS             <1..363
                     /locus_tag="PLV68_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01507.1"
                     /translation="VGIDLSWSTDLHLLVSFAVAHVLGLLVGFAFGAMCLNTPGALVG
                     LLAYDTVVHALIEVGAQRFDWFARLRPWIDFGEILVELSERGFAAVHWDQSLTSTALW
                     VGVPLAIGVRRLLHAEIK"
     gene            complement(399..527)
                     /locus_tag="PLV68_09415"
     CDS             complement(399..527)
                     /locus_tag="PLV68_09415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01508.1"
                     /translation="MTLPLIEVPPVAGGPIGCDGDTMSLGFTSGLADAAGVYTRIG"
     gene            complement(611..1198)
                     /locus_tag="PLV68_09420"
     CDS             complement(611..1198)
                     /locus_tag="PLV68_09420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01509.1"
                     /translation="MSRLTSFVVALLLMAACSSGGGPRAYFGSTIGADTQYGMQVPQG
                     WHRCEVSAACEQQTPDASAVMMSVYTYGNCSSPVGTDIARNLCLALDSGDVARCTTAV
                     AATYGSVASDTPALPITVIAWKNHPSSDFATTRFQAADLPKPFWQVTCARFLGNGQSM
                     VAVAMYARSSADLDSHQQVFVDTVASLPSLAPYNR"
     gene            1435..1713
                     /locus_tag="PLV68_09425"
     CDS             1435..1713
                     /locus_tag="PLV68_09425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01510.1"
                     /translation="MAKKPPARVINQFQRSASLDELTPANRIASELVTARLDLLPSIE
                     RIMNAGLDDATAATALDLFRTALTAPGDPHRDPRAAVTTAMAPTSASA"
     gene            1710..1961
                     /locus_tag="PLV68_09430"
     CDS             1710..1961
                     /locus_tag="PLV68_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01511.1"
                     /translation="MSRPKSHKRDREKARDERAAAKVARRLERRDSTEPTAAPVSSAE
                     QSEVLAGLAELHRRFSDGEIEFDDFDAAKRELTERLDVH"
     gene            complement(1998..>2222)
                     /locus_tag="PLV68_09435"
     CDS             complement(1998..>2222)
                     /locus_tag="PLV68_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01512.1"
                     /translation="NQQIAAALGDAGGSYELLIGSHICDALGVEPTHRGLLVPTPNIS
                     SAAAAWLRQAAEASDLESEHAAEVLAVLHL"
     assembly_gap    2223..2251
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2578..2631
                     /estimated_length=54
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3169..3245)
                     /locus_tag="PLV68_09440"
     tRNA            complement(3169..3245)
                     /locus_tag="PLV68_09440"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3209..3211),aa:Pro,seq:cgg)
     gene            complement(3263..>3595)
                     /locus_tag="PLV68_09445"
     CDS             complement(3263..>3595)
                     /locus_tag="PLV68_09445"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRE01513.1"
                     /translation="VTTAVEARTPDIAILDLQIGSMGGMAVTMNLRHDESSGALDHVP
                     VLMLLDREVDTFLARRSGADGWLVKPLDPFAITRAVHAVLNPPAPADGAAAVSTDESA
                     EEEAVAAG"
     assembly_gap    3596..3645
                     /estimated_length=50
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3680..>4426)
                     /locus_tag="PLV68_09450"
     CDS             complement(3680..>4426)
                     /locus_tag="PLV68_09450"
                     /inference="COORDINATES: protein motif:HMM:NF014433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HRE01514.1"
                     /translation="QEFMAAEKLYQLHLDERFDLVVVDTPPTRNALNFLDAPATLTRF
                     IDHKLFRLIMLPARSGLRVLNLAAQPLLRTIGKVVGGEVVADAIEFFQAFDGMESGFR
                     DRANAVSALLHSPATSYVLVASARHDTVEEATFFSGRLQESKVDVGAVVVNRLHPRFG
                     TASTAEAAARADELAASDPARSALWHNLATLGALADAEESVIAPLIAIAGPDALVRVP
                     LLRDDVHSVTGLGEMAGHLFAQVASPPCTS"
BASE COUNT          698 a         1403 c         1498 g          694 t
ORIGIN      
        1 gtcggcatcg acctctcgtg gtcgaccgac ctacacctgt tggtgtcgtt tgccgtcgct
       61 cacgtcctcg gcctgctcgt cgggttcgcg ttcggggcga tgtgcctgaa cacgcccggt
      121 gccctcgtcg gcttgctcgc gtacgacacc gtcgtgcatg cgctgatcga agtcggcgcg
      181 caacgattcg actggtttgc caggctgcgt ccgtggatcg acttcggtga gatcctcgtc
      241 gaactgtccg agcgaggttt tgccgccgtc cactgggacc aatccctcac ctcgactgcc
      301 ctctgggtgg gcgtgccgtt ggccatcggc gtgcgccgcc tcctccacgc cgagatcaag
      361 tagccatcag cgaccgtcac tggcgctgat cgcgatcctc acccgatacg ggtgtagacg
      421 ccagcagcgt cggcgaggcc agaggtgaac ccgagcgaca tcgtgtcgcc gtcgcatccg
      481 atcggtccgc cggccactgg cgggacctcg atcagcggca gggtcactcg attgatcgtg
      541 accccgcccc acgtcggaac ttcggagccc gccccctccc gatcccaccc gaccagcgga
      601 gcgtcgtgcc tcaacggttg tagggtgcca ggcttggcag gctcgccacg gtgtcgacga
      661 agacctgttg atgggagtcg agatcagcag atgagcgggc gtacatcgcg accgcgacca
      721 tcgactgacc gttcccgagg aagcgcgcac acgtgacctg ccagaacggc ttggggagat
      781 cggctgcttg aaagcgcgtg gtcgcgaagt cggagctcgg gtggttcttc catgcgatga
      841 cggtgatcgg cagcgccggc gtgtcggacg ccacgctgcc gtaggtggca gcgacagcgg
      901 tggtgcatcg cgcgacgtca cccgaatcga gcgccaagca gaggttgcga gcgatgtcgg
      961 tgccgactgg cgaggaacag ttgccgtagg tgtacacgct catcatgacc gccgacgcgt
     1021 ctggcgtctg ctgctcgcac gcggcggaga cctcgcagcg atgccacccc tggggaacct
     1081 gcatgccgta ctgggtatcg gcaccgatgg tcgacccgaa ataggcccgg ggtccgccgc
     1141 cagacgagca cgcggccatg agcagcagcg cgacgacgaa ggaggtgaga cgactcatcg
     1201 ccgaaccata cagggtgacc ctcgcccgtg tcggtggcca cgcgcactga cccattggtt
     1261 ggcggatagg ctgggggcag ttcttgggcg agtcgccccg cttgggcagg acggccagat
     1321 gcgctgtcgc cccgtgcagg cccgacgcct ccccgagccc tgagcccgag gagttgtcat
     1381 gcgcacgatc gagttgcgca ggttcgaccc gtccaaccag catcggagac cctgatggcc
     1441 aagaagccgc ccgcccgggt gatcaaccag ttccaacgat ctgcgtcgct cgacgaactg
     1501 accccggcca accggatcgc ctcggagctc gtgaccgcgc gactcgatct gttgccgtcg
     1561 atcgagcgca tcatgaacgc cggcctcgac gatgcgaccg cagcgaccgc gctcgacctg
     1621 ttccgcactg cgctgacggc gcccggcgat ccgcatcgcg atcctcgtgc ggccgtcacc
     1681 acggcgatgg ccccgacttc ggcgtccgca tgagtcgtcc gaagtcgcac aaacgtgacc
     1741 gtgagaaggc ccgcgacgag cgggccgcgg ccaaggtggc tcggcgactg gagcgacgtg
     1801 acagcaccga gccgactgcc gcgccggtgt cctcggccga gcagtccgag gtgctcgccg
     1861 gactggccga gctccaccgt cgcttctccg atggggagat cgagttcgac gacttcgacg
     1921 ccgccaagcg ggaactgacg gagcgcctcg acgtccactg actgagccga cgccgttccg
     1981 gggcgcaggg acgacggtca gaggtgcagc acggcaagga cctcggcggc gtgctcggat
     2041 tcgaggtccg acgcctctgc ggcctggcga agccacgcgg cggccgccga cgagatgttc
     2101 ggagtcggga ccaggagccc acggtgcgtc ggttcgaccc ccagagcgtc acagatgtgg
     2161 ctgccgatga gcagctcgta gctgccaccc gcatcgccga gcgcagcggc gatctgctga
     2221 ttnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncgctttcgg tcacgatcgg atggggacgg
     2281 tcaggtcggc ggccgcgacg ggactcgtgc ccgtttgaga aggaccgcaa tactggaatc
     2341 ggcgagtgtg ctcatcccga ccgcttccca accggcgtcg ccttcccggt tcaggagttc
     2401 gaccgatgcg gagtgtcctt gagttgcccg tgcgggctcg aacgtcggga gcgagacgat
     2461 gaggtactcg aagccggtca tgctgccagg ccgtcgactt cgccggactg catttgagcc
     2521 gcgatggtgt cgcagcgcca gcagcggaat cgtcggtcga gggggcgttg gtggaccnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngttggcgcc
     2641 gccccgtcgc gtctggacga tggcgacgac gccctcgtgg atgtccaggc cgggagcctg
     2701 atcgtcgtgg accgacacac ggtcaccgat cttcagcgcg ttccattgga aaggcagcat
     2761 cacggcgtcg tgttcggtag caggactgca cggcagggac cgcaggccca ccagtccgac
     2821 gcggttcgag ggtcggcgcc aagctcgagc gggaactcgt tgcggcaccg accacagcgc
     2881 cgcgaggcct ggggggtcga ttcgatggtc gcgggcatgt tgactccttg ctgtcgagca
     2941 gaggcgtcga gcctgtgtgc tgtgcggcga tctctcgtcc ggcgtcaacc acggcgtccc
     3001 tacggggtac ggagcactct accagcacag gaggacgagg aagccggcgt ctgggccgct
     3061 tctcgatcgg taccgaggat ggatcggtac atggcgaggt actggtcgac catcacggag
     3121 acgtcgaacc gttcgacgac agcgcgctcg gttcaagtca gagccaaatg gtcggggtga
     3181 caagattcga acttgcgacc tccacgtccc gaacgtggcg cgctaccaag ctgcgctaca
     3241 ccccgatggt gcgccggaga tgctatccgg ctgcgaccgc ttcttcctcc gccgattcgt
     3301 cggtgctgac ggctgcggct ccgtctgcgg gggcaggcgg gttgagcacg gcgtggacgg
     3361 cgcgggtgat ggcgaaggga tcgagcggct tgaccaacca gccgtcggcg ccgctgcgcc
     3421 gggcgaggaa agtgtcgacc tcgcgatcga gcagcatcag caccggcacg tgatcgagcg
     3481 cgccgctcga ctcgtcgtgg cgcaggttca tggtgaccgc catgccaccc atggaaccga
     3541 tctgcagatc gaggatcgcg atgtcgggag tgcgcgcttc gaccgccgtg gtgacnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnacggc gtcggcgacc
     3661 cacttggcgt cggtggcgat caggacgtgc acggcggcga ggctacctgg gcgaacaggt
     3721 gccctgccat ctcgccgagc ccggtgacgc tgtgcacgtc gtcgcgcaac aaggggacgc
     3781 gcacgagggc gtcaggtccg gcgatcgcga tcagcggcgc gatgaccgac tcctcggcgt
     3841 cggccagcgc gccgagggtg gccaggttgt gccacaacgc gctgcgcgcc gggtcggacg
     3901 cggccagctc atcggcgcgt gccgcggcct ctgcggtgct ggccgtgccg aaccggggat
     3961 gcagccgatt gaccaccacg gcgccgacgt cgactttcga ttcctggagc cgtccactga
     4021 agaacgtcgc ttcctcgacg gtgtcgtgac gcgccgacgc caccaggacg tagctggtcg
     4081 cgggcgagtg cagcagcgcc gagacggcgt tggcgcggtc acggaacccg ctctccatgc
     4141 cgtcgaaggc ctggaagaac tcgatcgcgt cggccacgac ctcaccgccg acgaccttgc
     4201 cgatcgtgcg cagcaacggt tgcgcggcca ggttgagcac gcgcaggccg ctgcgtgccg
     4261 gcagcatgat caggcggaac agcttgtggt cgatgaaccg ggtcagggtg gccggtgcgt
     4321 cgaggaagtt gagcgcgttg cgggtcggtg gggtgtcgac cacgaccagg tcgaaccgtt
     4381 cgtcgaggtg caactggtag agcttctcgg cggccatgaa ctcctg
//
LOCUS       DAORNF010000211         4423 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_14729_length_4423_cov_1.661401, whole genome shotgun sequence.
ACCESSION   DAORNF010000211 DAORNF010000000
VERSION     DAORNF010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4423
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14729_length_4423_cov_1.661401"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="PLV68_09455"
     CDS             complement(<1..353)
                     /locus_tag="PLV68_09455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01515.1"
                     /translation="MASNVEPSDLPRHLAHYGPRATVLTVSDAFAPHVGTSLVELIDE
                     RLVFSVGPSAAGHLAARPTLCLTWTPPPGEDYQLILDAIADAVTPQGGGFRVSAVVGR
                     GIRHRVADAAADGPS"
     gene            complement(368..913)
                     /locus_tag="PLV68_09460"
     CDS             complement(368..913)
                     /locus_tag="PLV68_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HRE01516.1"
                     /translation="MSDLQNIPLTTIDGSSTSLADFAGQVLLVVNVASKCGLTPQYTA
                     LEALYESKRDEGFAVLGFPANNFGAQEPGTESEISEFCSLNYGVQFPMFAKLSVSGDD
                     QHPLYAALTAAEPTKQGDPEAFRERLRGFGMTPAEDPEVLWNFEKFLIAKDGTVAARF
                     APTVAPDDALITAAIDAELAK"
     gene            986..>1315
                     /locus_tag="PLV68_09465"
     CDS             986..>1315
                     /locus_tag="PLV68_09465"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HRE01517.1"
                     /translation="MKPTGDTGPMSRTAPSGVYTPALDGLRALCIAAVVLYHLDPTGW
                     FDGGLHGVSVFFTLSGFLIVRNLVAERETTGGIDALRFWGRRVQRLAPAALATLVVVS
                     VITLLPDR"
     assembly_gap    1316..1335
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1336..>1955
                     /locus_tag="PLV68_09470"
     CDS             <1336..>1955
                     /locus_tag="PLV68_09470"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HRE01518.1"
                     /translation="LGRTIAAATWGLANWRMIDVYRTNVLAGLGPLAPMWSLAVEEQF
                     YLVVAVLFVVVRGRRWMATLAAVFATVIVASVAIALSLGDFEPRREFGTDVRAGELAA
                     GALLALALARNPEWIGRHRRTASIAGAVALGVALLVFVGIGPGHGWMTRGWIIGLAAV
                     SVVVITGLLADGPVATALSWRPLVALGRLSYSLYLVHWPVILLIRD"
     assembly_gap    1956..2100
                     /estimated_length=145
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2335..2408
                     /estimated_length=74
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2409..4112
                     /locus_tag="PLV68_09475"
     CDS             <2409..4112
                     /locus_tag="PLV68_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase dimerization domain-containing
                     protein"
                     /protein_id="HRE01519.1"
                     /translation="PNPLTVHPSLWRQAQLNNIHGLFEVAPGVWQARGYDISNITFVA
                     GATGWIVIDPLTVEGCARACLDLANAQLGQRPVVAVIYTHSHTDHFGGVHGVITPEEV
                     AAGDCRVIAPMGFMHEAIAENVIAGFAMQRRALYQFGILLPPGPAGHVDCGLGKCIPV
                     GPSGLIAPTEEITATGEELVVDGVRIVFQLTPESEAPAEMNFFFPDRVAPGKGWLCMA
                     ENCTHTMHNLVPIRGAQVRDALNWSKYIDESIDLYADAAEVMFASHHWPRWGNDDIRQ
                     FLRIQRDLYRYVHDQTMRHANHGLVATEIAEMMTLPPELARDAHATGYYGHLAHNVKA
                     VYQRYLSWYDGNPANLWKLPPLDAGRRYVDLAGGPDALLTAARAAYDAGEYRWVAELV
                     NHLVFADPTNTAARELQADTLEQLGFQATSATFRNAYLTGAQELRGGPLPRRDAMKRG
                     YLDAMTVEQLLDAIAVRLRAEDAGGGAATVNLIVAGEGAWRIALSNRALSTRPGAVPD
                     ADATLHVPRDVLVAISSNDTTIEKAIEDASVTVEGSRAAVEAIFAHLDTFQSMFPIID
                     P"
     gene            4142..>4423
                     /locus_tag="PLV68_09480"
     CDS             4142..>4423
                     /locus_tag="PLV68_09480"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HRE01520.1"
                     /translation="MGLVSLEDIRGAGPVVATVARRTPVITSVPLSGPAGDNVALKAE
                     NLQRTGSFKLRGAINKVVSLGDTALAGVTTGSAGNHAQALAYAAHRVGAP"
BASE COUNT          602 a         1389 c         1505 g          688 t
ORIGIN      
        1 cagctggggc cgtcggccgc tgcgtcggcg acgcggtggc ggatgccgcg gccgaccacg
       61 gcactgacgc ggaaccctcc gccctgcggt gtcactgcgt cggcgatggc atcgaggatc
      121 aactggtagt cctcgcccgg cggtggggtc cacgtcaagc acagggtggg gcgcgcggcc
      181 aagtggccgg ccgcgctcgg cccgacgctg aacaccagcc gctcgtcgat cagttcgacc
      241 agcgacgtgc cgacgtgagg cgcgaacgcg tcggagaccg tcagtaccgt cgcccgcggt
      301 ccgtagtggg cgaggtgacg gggcaggtcg ctcggttcga cgttgctggc cacggactgc
      361 ggtccgacta cttggcgagt tcggcgtcga tcgcggcggt gatcagcgcg tcgtcgggag
      421 cgaccgtggg ggcgaagcgg gccgcgaccg tgccgtcctt ggcgatgagg aacttctcga
      481 agttccacag cacctcggga tcctcggccg gcgtcatgcc gaagccgcgc agccgctcgc
      541 ggaacgcctc ggggtcgccc tgcttggtgg gctcggccgc ggtgagcgcg gcgtacagcg
      601 ggtgctggtc gtcgccgctg acgctgagct tggcgaacat cgggaactgc acgccgtagt
      661 tgagcgagca gaactcgctg atctccgact cggtacccgg ctcttgcgcg ccgaagttgt
      721 tggcggggaa gccgagcacg gcgaagccct cgtcgcgctt ggactcgtac agagcttcga
      781 gcgcggtgta ctgcggggtg agcccgcact tcgacgcgac gttgacgacc aacaggacct
      841 gtccggcgaa gtcggcgagg ctggtggagg aaccgtcgat ggtggtgagg gggatgttct
      901 gcagatcgct catagctgcc attctggcgc actcgtcaac gttgccgcgc ccgcgaccga
      961 tcaggcctac taccgtgccc acccgatgaa gccgacgggt gacacggggc cgatgtcgcg
     1021 gacagcgccg tcgggcgtct acaccccagc cctcgacggc ctgcgcgcgc tgtgcatcgc
     1081 cgccgtcgtg ctgtaccacc tggacccgac cggctggttc gacggcgggt tgcacggtgt
     1141 gtcggtgttc ttcacactgt cgggcttcct gatcgtgcgc aacctggtcg ccgagcggga
     1201 aacgaccggc ggtatcgacg ccctgcgctt ttggggacgg cgggtacagc gcctggcccc
     1261 ggcggcgctg gccacactgg tggtggtcag cgtgatcacg ctgttgcccg atcggnnnnn
     1321 nnnnnnnnnn nnnnngctcg gtcgcacgat cgccgcggcg acgtggggat tggccaactg
     1381 gcggatgatc gacgtctacc gcaccaacgt gttggccggg ttggggccgc tggccccgat
     1441 gtggtcgttg gccgtggagg agcagttcta cctggtggtc gcggtgctgt tcgtggtggt
     1501 gcgcgggcgg cgctggatgg cgacgttggc cgcggtgttt gcgacggtga tcgtcgcctc
     1561 cgtcgcgatc gcgctgagcc tgggcgactt cgaaccgagg cgcgagttcg gcaccgacgt
     1621 ccgcgctggc gaactggctg cgggtgcgct gctcgcactc gcgttggcgc gcaacccgga
     1681 gtggatcggt cgccaccgcc gtaccgccag catcgccggt gcggtcgcgc tcggcgtcgc
     1741 actgttggtg ttcgtcggga tcgggccggg tcacggctgg atgacgcgcg gttggatcat
     1801 cggccttgcc gcggtgagtg tcgtggtgat caccggtctg ctcgccgacg gtccggtggc
     1861 gaccgcgctg agttggcgcc cgctggtcgc gctcggccgg ttgagctaca gcctgtacct
     1921 cgtccactgg ccggtgatcc tgctgatccg cgatcnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 cgcctcggcg gtgctcagcg cggtggtgat cgtggcgttc tgacccgcgt cggcgatgcg
     2161 ctgtcagggg tgcggggctc cgactcttgt cagtggtact gacaggtacc acgatggggt
     2221 catgcaccag cccacagcgc acaccgccgc gcacaacgcg ccgcgcgatc tcggtatgga
     2281 tccggacgac ttcgagcggg ccagccgcgg tctgatcgcc cagcatccga ccggnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnncc caatccactg accgtgcatc ccagcctgtg gcgccaggcg cagttgaaca
     2461 acatccacgg cttgttcgaa gtggcgccgg gggtgtggca ggcccggggc tacgacatct
     2521 ccaacatcac cttcgtcgcc ggtgcgaccg gctggatcgt gatcgacccg ctgacggtcg
     2581 agggctgtgc ccgggcgtgt ctcgatctgg ccaacgcgca gctcggtcag cgtccggtgg
     2641 ttgcggtgat ctacacccac agccacaccg accacttcgg tggggtgcac ggagtgatca
     2701 cgccagagga ggtcgcggcg ggcgactgcc gcgtcatcgc cccgatgggt ttcatgcacg
     2761 aggcgatcgc ggagaacgtg atcgccgggt tcgcgatgca gcgccgcgcc ctgtatcagt
     2821 tcggcatctt gctgccgccg ggtccggcgg gccacgtcga ctgcggcctg ggcaagtgca
     2881 ttccggtcgg tccgtcgggt ctgatcgcgc cgaccgagga gatcaccgcc accggcgagg
     2941 aactcgtggt cgacggggta cggatcgtgt tccaactgac gccagagtcg gaagcgccgg
     3001 cggagatgaa cttcttcttc cccgatcgcg ttgcgcccgg caagggctgg ctgtgcatgg
     3061 ccgagaactg cacccacacc atgcacaacc tggtgccgat ccgtggcgcg caggtccgcg
     3121 acgcgctcaa ctggtcgaag tacatcgacg agtcgatcga cctctacgcc gatgcggccg
     3181 aggtgatgtt cgccagccac cactggccgc ggtggggcaa cgacgacatc cgccagttcc
     3241 tgcgcatcca gcgcgacctg tatcgctacg tgcacgacca gacgatgcgt cacgccaacc
     3301 acggcctggt cgcgaccgag atcgccgaga tgatgacgct gcccccggag ttggctcgcg
     3361 acgcccacgc caccgggtac tacggccacc tcgcgcacaa cgtgaaggcg gtgtaccagc
     3421 gttacctcag ttggtacgac ggaaatcccg cgaacctgtg gaagttgccg ccgttggacg
     3481 cgggtcgccg ctacgtcgat ctggccggtg ggccggacgc gctgctgacc gcggcgcggg
     3541 ccgcgtacga cgccggggag taccgctggg tcgccgagct cgtcaaccat ctggtgttcg
     3601 ccgaccccac caacacggcc gctcgcgagt tgcaggccga caccctggag cagctcggct
     3661 tccaggccac ctcggccacg ttccgcaacg cgtacctcac cggcgcgcag gagttgcgcg
     3721 gcggaccgtt gccgcggcgc gacgcgatga aacgcggcta cctcgacgcg atgacggtcg
     3781 agcagttgct cgatgcgatc gcggtgcgcc tgcgcgccga ggacgcgggc ggcggcgcag
     3841 cgaccgtcaa cctgatcgtc gccggtgagg gcgcgtggcg gatcgcgctg tcgaaccgcg
     3901 ccctcagcac ccgccccggc gcggtacccg acgccgatgc caccctccac gtcccccgcg
     3961 acgtgttggt tgcgatcagc agcaacgaca caaccatcga gaaggcgatc gaagacgcca
     4021 gcgtcaccgt cgaagggtcc cgagcggccg tcgaggccat cttcgcccac ctcgacacct
     4081 tccagtccat gttccccatc atcgacccct gagcaggcgg tcgggcgcgt acggtggggg
     4141 tgtgggcttg gtgagcttgg aggacattcg aggggcggga ccggtcgtgg cgacggtggc
     4201 gcggcgcacg ccggtgatca cgtcggtgcc gctgtcgggt ccggctggcg acaacgtcgc
     4261 gttgaaggcg gagaacttgc agcgcaccgg gtcgttcaag ctgcgcgggg cgatcaacaa
     4321 ggtcgtgtcg ctgggcgata ccgcgttggc cggggtcacc acgggcagtg cgggcaatca
     4381 cgcccaggcg ttggcctacg cggcccaccg ggtcggcgcg ccg
//
LOCUS       DAORNF010000212         4423 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_14730_length_4423_cov_1.368590, whole genome shotgun sequence.
ACCESSION   DAORNF010000212 DAORNF010000000
VERSION     DAORNF010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4423
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14730_length_4423_cov_1.368590"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="PLV68_09485"
     CDS             complement(<1..190)
                     /locus_tag="PLV68_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRE01521.1"
                     /translation="MTIDRPQARNAVDGPTARSLFEAFREFEADESAHVAILTGAGGT
                     FCAGADLKAIGSGNGNRVE"
     assembly_gap    209..396
                     /estimated_length=188
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <397..>1375
                     /locus_tag="PLV68_09490"
     CDS             <397..>1375
                     /locus_tag="PLV68_09490"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRE01522.1"
                     /translation="DRIVAVGTIASRGRREIDADGMIVTPGFVDIHTHFDGQATWDPE
                     LRPSSQHGVTTVAFGNCGVGFAPARPDRHDWLISLLEGVEDIPGSALSEGMTWGWESF
                     PEYLDVLASMPRTVDVATHVPHAALRTYVMGDRGADHLQHPDDDEIATMARLVGEALE
                     AGAIGFATSRTEIHRTSAGANIGTLRAGERELLAIADAMRSADRGVIQLISDCYLSKD
                     ADFAETEIDLIGAIARTSARPLSFTVQQAAEVPERWRRLFGAVDTWRAAGLDVKGQVA
                     PRPIGVLLGLQATTNPFVICPSYTEIASLPLAQRVQRMADPQRKAAILAE"
     assembly_gap    1376..1579
                     /estimated_length=204
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1580..>1792
                     /locus_tag="PLV68_09495"
     CDS             <1580..>1792
                     /locus_tag="PLV68_09495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HRE01523.1"
                     /translation="LLYLPLFGFAHRSLADIHEMIIQPGSLYGLSDAGAHCGAICDAS
                     NSTSFLTVWARDCDESRRLPIEFVVH"
     assembly_gap    1793..1988
                     /estimated_length=196
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2213..2350
                     /estimated_length=138
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2396..2629)
                     /locus_tag="PLV68_09500"
     CDS             complement(2396..2629)
                     /locus_tag="PLV68_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01524.1"
                     /translation="MPSSSTSTTIRVSKPVRDELGALAAAAHLTLDELLRRLARAERQ
                     RIIADEQATYEPTEADRAVIAAGLGTVTRHAVG"
     gene            complement(2648..>3246)
                     /locus_tag="PLV68_09505"
     CDS             complement(2648..>3246)
                     /locus_tag="PLV68_09505"
                     /inference="COORDINATES: protein motif:HMM:NF014476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01525.1"
                     /translation="SVAAVHPALTRFAAPLHVVVFGAGPQAVGHVVTLAAVTAAPLAS
                     VTFVVRHPNRVGHDVTSLGAVVGADSPAATEAVGRADVVVCATSARTPLFRGEIVASN
                     VIVIAVGSHEPDARELDSALLARSVVVVEDVGAALREAGDVIAALSEEAITRDALIPM
                     TMAISGATPLPPDRPLVFKSVGMSWQDLTVATAILAAA"
     assembly_gap    3247..3464
                     /estimated_length=218
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3564..>4423)
                     /locus_tag="PLV68_09510"
     CDS             complement(3564..>4423)
                     /locus_tag="PLV68_09510"
                     /inference="COORDINATES: protein motif:HMM:NF017365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="proline racemase family protein"
                     /protein_id="HRE01526.1"
                     /translation="EPRGHADMYGGFVVPPDDDGAHFGVLFWHKDGFSTACGHGTIAL
                     GAWAIDSGVVARDPSGVTDVVIDVPSGRVTARVHTAADGAVEAVDFVNVPSRLVASGV
                     IVPTSKGNVRVELGWGGALYGFVDAASVDVTVTPEHVTELIALGREIKAALFADPRLE
                     HPADPRLSGVYGTIFTEDFGTLPTGELHQRNVTIFADGQVDRSPCGSGTAARIAVLHA
                     HGELGDEQVLVHDSIVGSRFRARVLEQTTEFGQPAVVPIVTGNAYRVGRSIFEVDPRD
                     PLTPGFVLR"
BASE COUNT          588 a         1249 c         1141 g          501 t
ORIGIN      
        1 gctcgacccg gttgccgttg ccgctgccga tcgccttgag gtcggctccc gcgcagaacg
       61 tgccaccggc tccggtgagg atcgcgacat gggccgattc gtccgcctcg aactcgcgaa
      121 acgcctcgaa cagcgaacgc gccgtcggcc cgtcgaccgc gttgcgagcc tgcgggcgat
      181 cgatcgtcac gatccggacg tggccgtcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngatc gcatcgttgc agtcggaacg
      421 atcgcgtcgc gtggccgccg cgagatcgac gccgacggca tgatcgtcac acccggcttc
      481 gtcgacattc acacccactt cgacggtcag gccacctggg accccgagct tcggccctcg
      541 tcgcaacacg gcgtcaccac ggtggcgttc ggcaactgtg gcgtcgggtt cgcgccagcc
      601 cggcccgatc gtcacgactg gctgatcagc ctgctcgaag gtgtcgagga catccccggc
      661 tccgccctca gcgaaggcat gacctggggt tgggaatcgt tccccgagta tctcgacgtc
      721 ctcgcgtcga tgccccgcac cgtcgacgtc gcgacccacg tcccgcacgc cgcgctgcgc
      781 acctacgtga tgggcgaccg cggtgctgat cacctccagc atcccgacga cgacgagatc
      841 gcgacgatgg cccgcctcgt cggcgaggcg ctcgaggccg gagcgatcgg gtttgcgacg
      901 tcgcgcaccg agatccaccg caccagcgcc ggagccaaca tcggcaccct gcgggccggc
      961 gagcgtgagc tgctcgcgat cgcggacgcc atgcgcagtg ccgatcgcgg cgtgatccaa
     1021 ctgatctccg actgctacct cagcaaggat gccgacttcg ccgagaccga gatcgacctc
     1081 atcggtgcca tcgcccgcac ctcggcccgg ccactgagct tcacggtgca acaggcagcc
     1141 gaggtccccg agcgctggcg gcgcctgttc ggtgccgtcg acacctggcg cgccgcgggg
     1201 ctcgacgtca aaggtcaggt cgcgccacgg ccgatcggcg tgctgctcgg tctccaggcc
     1261 accaccaacc cgttcgtgat ctgcccgagc tacaccgaga tcgcatcgct gccgttggcg
     1321 cagcgggtgc aacggatggc cgatcctcaa cgcaaggcgg cgatcctcgc cgaacnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnng gctgctgtac ctcccgctgt tcggcttcgc gcatcgcagc
     1621 ctggccgaca tccacgagat gatcatccag ccgggctcgc tctatggcct cagcgacgcc
     1681 ggcgcgcact gcggcgcgat ctgcgatgcg agcaactcca cctcgttcct cacggtctgg
     1741 gcgcgcgact gcgacgagtc gcggcggttg ccgatcgagt tcgtcgtcca tcnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnncc acgatcaagt ccggcaatgt gacgatgctc gacggcaacc ccaccggcat
     2041 ccgacccgga accctccttc gcggcaccac cccttccccc acctaggaaa gatcgagcaa
     2101 gtcggcaatc acagaccgca actgcgccaa cgcagccgac ccgacattgc ctcgcccgcc
     2161 gtcgtcgagc acccgctcgg cctccgtgac ggtcatcaag tgacattgag cgnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn ccggtaccgc tccccatggg aagaccgaag tcgaccgtga tcacgtcacc
     2401 cgaccgcatg ccgcgtcacc gtgccaagtc cagcggcgat gaccgcacga tcggcctcgg
     2461 tcggctcgta cgtcgcctgc tcatcagcga tgatccgctg gcgctccgcg cgtgcgagac
     2521 gacgcaacag ctcgtcaagc gtgagatgtg cggctgctgc cagcgcaccg agttcgtcac
     2581 gcaccggctt ggagacacgg atcgtggtcg atgtagacga agatggcata cagaacaggc
     2641 tatgcactca tgctgccgcg aggatggcgg tggcgacggt gaggtcttgc caggacatgc
     2701 cgacactctt gaacaccagc ggacggtcgg gtgggagcgg ggtcgcgccg gagatcgcca
     2761 tggtcatcgg gatcagcgcg tcgcgggtga tcgcttcctc ggacagcgcg gcgatcacgt
     2821 cacccgcttc gcgcagcgcg gcaccgacgt cttccacgac gacgacggaa cgagccaaca
     2881 gcgcgctgtc gagttcgcgg gcgtcgggtt cgtgcgagcc gacggcgatc acgatcacgt
     2941 tcgacgcgac gatctcgccg cgaaacagcg gcgtgcgcgc gctggtggca cagaccacca
     3001 catcggcgcg gccgaccgcc tcggttgccg cggggctgtc ggcgccgaca accgcgccga
     3061 gcgaggtcac gtcgtgtccc actcggttcg gatggcggac cacgaacgtc accgacgcca
     3121 acggcgcagc ggtaaccgcc gccaacgtga cgacgtggcc gaccgcttgc ggaccggcgc
     3181 cgaagacgac cacgtggagc ggagcggcga agcgagtcag ggcggggtgg acggcggcca
     3241 cggacannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnggtcga agccgtcgat
     3481 gaccacgcgg gtgatggcgt cgaccgccgc ccgcggcgac aacgccgccg cgatcgcggc
     3541 cgcgtcgaag tcgcgcgcca cgctcaccgc aacacgaaac ccggcgtgag cgggtcccgc
     3601 gggtcgacct cgaagatcga acgtccgact cggtacgcgt tgccggtcac gatcggcacg
     3661 acagcgggct gaccgaactc ggtggtctgc tccaaaacgc gagcacgaaa gcgcgacccg
     3721 acgatcgagt cgtggacgag gacctgttca tcgccgagtt cgccgtgggc gtgcaacacg
     3781 gcgatgcgcg ccgcggtgcc cgacccgcac ggtgagcggt cgacctggcc gtcggcgaag
     3841 atcgtcacgt tgcgctgatg caactcgccg gtcggcagcg tgccgaagtc ctccgtgaag
     3901 atcgtgccgt agacgccgct gaggcgcggg tcggcgggat gctcgaggcg cgggtcggcg
     3961 aagagcgcgg ccttgatctc tcgaccgagc gcgatcagtt cggtcacatg ctctggggtc
     4021 accgtcacgt cgaccgaggc ggcatcgacg aagccgtaca gcgcaccgcc ccaaccgagc
     4081 tcgacacgca cgttgccttt gctggtcggc acgatcaccc ccgaggccac caagcggctc
     4141 ggcacgttca caaagtcgac cgcctcgacc gcgccgtcgg ccgcggtgtg aacgcgggcg
     4201 gtcacccgac cggacggtac gtcgatcacc acatcggtca cgccagacgg atcgcgagcg
     4261 acgacgccgc tgtcgatcgc ccacgccccg agcgcgatcg tgccgtgccc acatgccgtc
     4321 gagaagccgt ccttgtgcca gaacagcacg ccgaagtggg cgccgtcgtc gtcgggcggt
     4381 acgacgaagc cgccgtacat gtcggcatgc ccgcgcggct cga
//
LOCUS       DAORNF010000213         4414 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_14775_length_4414_cov_1.813948, whole genome shotgun sequence.
ACCESSION   DAORNF010000213 DAORNF010000000
VERSION     DAORNF010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4414
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14775_length_4414_cov_1.813948"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..839
                     /locus_tag="PLV68_09515"
     CDS             <1..839
                     /locus_tag="PLV68_09515"
                     /inference="COORDINATES: protein motif:HMM:NF025008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HRE01527.1"
                     /translation="PHPTPPGFDMTTTFARATTVTPRGPGRYGVDLAREYSIMGAKPN
                     GGYILACLARATLHAAHEAGASQRQIIAATTQYLSSPDLGPAEITTAVDRIGRSASQV
                     TATIGTDGAVGVRAQFTLGTLHEASTPYWGAVPPVAMPPIEQCELLQMADGREEQVLF
                     DPATTFRVTSDGLVVTGDGEFRAWFRDDGAGMFDSVGLMYASDVLPPATFGVLNTGWV
                     PTLSLTVYNRAIPAPGPLQLRFRVQVIQDGYADEALDAWDSTGRLVMQATQLAALRLP
                     QA"
     gene            complement(841..2001)
                     /locus_tag="PLV68_09520"
     CDS             complement(841..2001)
                     /locus_tag="PLV68_09520"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRE01528.1"
                     /translation="MISREIRQRLTSRSFAVTTVLLCVGIIGAGVLHRALSGDDSPEK
                     YDVAVIGTAPDGFPYGLGVAAHAVGIEVTVDPIDDRAVAERSIRDGDVDVAIDAHRAE
                     ILAKDTVPDELAVAVDVAWRSARSHAAAVEAGLDANQITAIVSPAPLTAVALEPDADA
                     DDGVGMVVGFAMAILLFISINAFGGAVLTGVVEEKTTGVVEVLLAHLRAHQLLTGKVL
                     GIGAVALVQLTAAVGAGLVALRIAGTAVPAAVWVSLPATLLWFVFGFVLYSTLFALAG
                     SCVSRQEDAQGAAAPISIVFTAAYLTVFVLGAVPASTATRIVSVLPPFAPLLMPLRIA
                     VGAASVVEIVVAAVALIGATVGMLRLAGAVYGATLLHRGSRLSWKQALRMRG"
     gene            complement(2025..2954)
                     /locus_tag="PLV68_09525"
     CDS             complement(2025..2954)
                     /locus_tag="PLV68_09525"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRE01529.1"
                     /translation="MLAVDSISKRFGDVVALDQVSLQVSSGQIVGFLGPNGAGKSTTM
                     RAIMGLIAVDGGSVTWNDLSLDAERRRRFGYMPAERGMYPKMTVADQIVYFARLTGMG
                     ASDAKAAAERWMQRVDIASRRDDEVQALSSGNQQRVQLAIALVHEPDLLILDEPFSGL
                     DPVAVENMKQIILDQVGRGTAVLFSSHQLDLVSDISRDVVIIDGGRVVLHGDVRDLRD
                     HSESRTATVTFAAPVSWTPRVAGAVVTRAGEETVVVRVPRTVGPADILADAATAGEVA
                     EFSFAPPDLSEVFLAAVGRTTMGQDEAIGERVS"
     gene            3086..>3244
                     /locus_tag="PLV68_09530"
     CDS             3086..>3244
                     /locus_tag="PLV68_09530"
                     /inference="COORDINATES: protein motif:HMM:NF013104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01530.1"
                     /translation="MTEEQVAELVNEAGSSGNIAWILAAAALVLFMTPGLAFFYGGMD
                     RTKNVLNML"
     assembly_gap    3245..3362
                     /estimated_length=118
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3363..4336
                     /locus_tag="PLV68_09535"
     CDS             <3363..4336
                     /locus_tag="PLV68_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HRE01531.1"
                     /translation="FMRDIDLSSPAGLATIAFLGMFAVITPALISGAVADRMKFSAWA
                     IFVPLWLFLAFIPVFKWVYGGWLGQRGSIDFAGGTAIHINAGIAALAAVLVLGKRKNW
                     PNDVQPPHSMPLVMLGTGILWFGWFGFNAGSALAANGQAVQAFMNTFLAAAAAGLAWC
                     VVERIRDGHFTNLGAASGIVAGLVAITPCAGFVAGMSPIWIGLIAGVVCCYAVKLKVT
                     FGYDDALDVVGVHFVGGLVGTLLLGLFADPEFFGGEFLKGTFYGGGMQLFGEQALAAL
                     VSIVWSFIITFGIMIALKKTIGVRVDDETEVVGLDLSEHHETAYHSG"
BASE COUNT          725 a         1503 c         1364 g          704 t
ORIGIN      
        1 cgcctcaccc cactccccca ggattcgaca tgacgaccac ctttgcccga gcgaccacgg
       61 tgacgccgcg cggccctggc cgctacggcg tcgatctcgc ccgcgagtac tcgatcatgg
      121 gcgccaagcc caacggcgga tacatcctgg cgtgtctggc cagggccacg ctccatgcgg
      181 cacacgaggc aggggcgagc cagcgccaga tcatcgccgc cactacccaa tacctgtcct
      241 cacccgatct cggcccagcc gagatcacca cggcggtcga tcgcatcggt cgctcggcct
      301 cgcaggtcac agcgacgatc ggtaccgatg gcgcggtcgg cgtccgtgcc cagttcaccc
      361 tcggcactct gcacgaggcc agcacgccgt attggggtgc ggtgccgccg gtggcgatgc
      421 cgccgatcga gcagtgcgaa ctactccaga tggccgacgg gcgcgaggag caggtgctgt
      481 tcgaccccgc gaccacattt cgggtgacgt ccgacggact ggtggtgacc ggcgacggcg
      541 agttccgtgc ctggttccgc gacgacggtg ccggcatgtt cgactcggtc ggtctgatgt
      601 atgcctcgga cgtgctgcct ccggccacgt tcggtgtgct gaacaccggt tgggttccga
      661 ccctgtcgct cacggtgtac aaccgggcca ttcccgcccc cggtccgctc caactgcgct
      721 tccgggtgca ggtcatccag gacggctacg ccgacgaggc gctcgacgcg tgggactcga
      781 ccggccggtt ggtgatgcag gccacccaac tcgccgcctt gcgcttgccg caggcctgac
      841 tcagccgcgc attcgcagcg cctgcttcca cgacaagcgg ctgccccgat gcagcagcgt
      901 cgcgccgtag accgcaccag ccagacgcag catgcccacc gtggccccga tcagcgccac
      961 cgcggcgacg acgatctcca cgaccgacgc ggcgccgacc gcgatccgca acggcatcag
     1021 cagtggggcg aatggcggca ggaccgacac gatgcgagtc gcagtgctgg ccggaacggc
     1081 accaagcacg aacaccgtca agtacgcggc ggtgaacacg atcgagatcg gcgccgccgc
     1141 gccttgagcg tcttcttgcc gcgacacgca tgacccggcc agcgcgaaca acgtgctgta
     1201 gagcacgaag ccgaacacga accacagcag cgtcgcgggc aggctgaccc agacggcagc
     1261 cggcacggca gtccctgcga tgcgcagtgc caccagtccc gcaccgaccg cggctgtcaa
     1321 ctgcaccaac gcgaccgcgc cgatgccgag caccttgccg gtgagcagtt ggtgggcgcg
     1381 cagatgggcc agcaacacct cgacgacgcc cgtcgtcttc tcttcgacca cgccggtcag
     1441 caccgctccg ccgaacgcgt tgatcgagat gaacagcaag atcgccatcg caaatcccac
     1501 gaccatgccg acgccgtcgt cggcgtcggc gtcaggttcg agcgccacgg cggtgagggg
     1561 cgcggggctg acgatcgctg tgatctggtt ggcgtcgagc cccgcctcga cggccgcagc
     1621 gtgcgaccgg gccgaccgcc atgccacgtc gacggccacg gcgagctcgt cgggcacggt
     1681 gtccttggcg aggatctcgg cccggtgggc gtcgatcgcg acatcgacat caccgtcgcg
     1741 gatcgagcgc tcggccaccg cccggtcgtc gatgggatcg accgtgacct cgatgccgac
     1801 cgcgtgcgcg gcgacgccca gcccgtaggg aaaaccgtcc ggcgcggtac cgatcacggc
     1861 gacgtcgtac ttctccggcg agtcgtcacc gctgagggcg cggtgcaaca ccccggcacc
     1921 gatgatgccg acgcacagca acaccgtcgt gaccgcgaag ctgcgcgagg tgagccgctg
     1981 acggatctca cgcgagatca ccaagccgat ggtcgccagc ccgctcacga cacccgctct
     2041 ccgatcgcct cgtcctgccc catggtggtg cggccgaccg cggccagaaa cacctcggac
     2101 agatccggtg gcgcgaagct gaactcggcc acctcaccgg cggtggcggc atccgccagg
     2161 atgtcggccg ggccaaccgt gcgcggcacc cgcacgacca ccgtctcttc gccggcacgt
     2221 gtcaccacgg cgcctgcgac ccgcggtgtc caagagaccg gggcggcgaa ggtcaccgtg
     2281 gccgtgcgcg actcgctgtg gtcgcgcagg tcgcgcacgt cgccgtgcaa caccacgcgg
     2341 cctccgtcga tgatcaccac gtcgcgactg atatcgctga ccagatcgag ttggtggctg
     2401 ctgaacagca ctgccgtacc gcgcccgacc tgatcgagga tgatctgctt catgttctcg
     2461 accgccaccg gatcgagacc ggaaaacggt tcgtcgagga tcagcaggtc gggttcgtgc
     2521 accagcgcga tcgccaactg cacccgttgc tggttgccgc tggacagcgc ctgcacctcg
     2581 tcgtcgcgtc gcgacgcgat gtcgacccgc tgcatccagc gctcggcggc ggccttggca
     2641 tcggatgcac ccatgccggt caaccgggcg aagtagacga tctgatcggc aacggtcatc
     2701 ttcgggtaca tcccccgttc ggccggcatg tagccgaacc ggcggcgccg ctcggcgtcg
     2761 agtgagaggt cgttccatgt gacagaaccg ccgtcgacgg cgatcaaacc catgatcgct
     2821 cgcatcgtcg tgctctttcc cgcaccgttg ggcccgagga agccgacgat ctgtccgctg
     2881 ctgacctgca ggctcacctg gtcgagcgcc accacatcac cgaaccgctt ggagatcgaa
     2941 tcgaccgcga gcatccgagt caccctatgc agttgccgac gattggacac atcggcttca
     3001 tcggcatgca tcgcacagga aacacgacag aaacacgcct ttgccattct gcgcacgacg
     3061 aaatgtcttg acctcggagg actgcatgac tgaagaacaa gtggctgaac tggtgaatga
     3121 agccggaagc agcgggaaca tcgcctggat cttggcagcg gccgcattgg tgctgttcat
     3181 gacccctggc ctggccttct tctacggcgg catggatcgc accaagaacg tcctcaacat
     3241 gttgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nncgttcatg cgtgacatcg atctgagctc accggcgggg ttggccacga tcgccttcct
     3421 cggcatgttc gccgtgatca cccctgcgct gatctccggt gccgtggccg accggatgaa
     3481 gttctcggcg tgggcgatct tcgtgccgct gtggctgttc ctggccttca tcccggtctt
     3541 caagtgggtc tacggcggct ggctgggcca gcgcggctcg atcgactttg ccggtggcac
     3601 cgcgatccac atcaacgccg gtatcgccgc cctcgccgcc gtgctggtgc tgggcaagcg
     3661 caagaactgg cccaacgacg tgcagccgcc gcactcgatg cccctggtca tgttgggcac
     3721 cggcatcctg tggttcggtt ggttcggctt caacgccggc tccgcactcg cagccaacgg
     3781 ccaggccgtc caggccttca tgaacacctt cctcgctgcc gcggcggccg ggctggcctg
     3841 gtgcgtggtc gagcgcatcc gtgacgggca cttcaccaac ctcggtgctg catcgggcat
     3901 cgtcgccggt ctggtggcga tcacaccgtg cgccggcttc gtggcgggca tgagcccgat
     3961 ctggatcggt ctgatcgccg gcgtggtctg ctgctatgcg gtcaagctca aggtgacgtt
     4021 cggttacgac gatgccctcg acgtcgtcgg cgtccacttc gtcggcggtc tggtcggcac
     4081 cctgctgctc ggcctgttcg ccgacccgga gttcttcgga ggcgagttcc tgaagggcac
     4141 gttctatggt ggcgggatgc agctgttcgg cgaacaggcc cttgcggcgc tggtgtcgat
     4201 cgtctggtcc ttcatcatca cctttggcat catgatcgcc ctcaagaaga cgatcggcgt
     4261 gcgggtcgac gacgagaccg aagttgtcgg actcgacctg agcgagcacc acgagacggc
     4321 ctaccactcg ggctgatcac acgagacacg acaagaggag cacaaccatg aaactcatca
     4381 ccgcagtgat caagccgttc aaactggccg gtac
//
LOCUS       DAORNF010000214         4408 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_14809_length_4408_cov_1.560763, whole genome shotgun sequence.
ACCESSION   DAORNF010000214 DAORNF010000000
VERSION     DAORNF010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4408
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14809_length_4408_cov_1.560763"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(108..>667)
                     /locus_tag="PLV68_09540"
     CDS             complement(108..>667)
                     /locus_tag="PLV68_09540"
                     /inference="COORDINATES: protein motif:HMM:NF024588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PDGLE domain-containing protein"
                     /protein_id="HRE01532.1"
                     /translation="SGLAAVCSVVLAAVAFSIEWLFGASAPVPFDRVFGAMVGVHALI
                     GLGEGVITAGAIGAVLGSRPDLVAGAHDLSPEQSADRGGLGLRPFVIGGCIVALVCAS
                     VIGQFAFDNPDGLERVAIDHGFAQAGTDHALASSPLADYATQGVGNETVSLAIAGAVG
                     VALSLIVISGVLAATRPLRRRGAPA"
     assembly_gap    668..677
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<678..1102)
                     /locus_tag="PLV68_09545"
     CDS             complement(<678..1102)
                     /locus_tag="PLV68_09545"
                     /inference="COORDINATES: protein motif:HMM:NF014007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000041 - transition metal ion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HRE01533.1"
                     /translation="MSALVAMHAPDGFLEVPVAVATAVIAAGFVTAALAVARRHLGDR
                     QIPLAGVAAAFIFAAQMFNFPVAAGTTGHLLGGALAAILLGPWLGLLVVTVVVVIQAL
                     FFADGGLTALGYNVVNMAVVPAFAGWALFRLFRRWLAPNR"
     gene            complement(1113..2387)
                     /locus_tag="PLV68_09550"
     CDS             complement(1113..2387)
                     /locus_tag="PLV68_09550"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HRE01534.1"
                     /translation="MPTSSSPDSHAAAVTPRRVSQRLAAINESATLAVDAKAKALQAQ
                     GENVIGFGAGEPDFPTPDHIVEAAVAACRDTKNHRYTPAAGLPALREAIAAKTLRDSG
                     FATTANQVLVTNGGKHAVYTAFAALCDPGDEVIVPAPYWTTYPEAITLAGGVPVVIDT
                     TEETSFRVTVEQLDAALTSRTKVLLFVSPDNPSGAVYSPEEVEAIGRWAVEKGVWVVT
                     DEIYEHLTYGPHRFVSMPTVVPDLADQCLIVNGVAKTYAMTGWRVGWMIGPSDVIKAS
                     VNFQSHATSNVSNVAQQAALAAVAGDLSAVHMMRTAFERRGKAMHGMLNAIDGVTCIE
                     PQGAFYCFANMQGLLGRDLRGHVAHSTLELADIKYASELGYTIKPRWRSCRVRRSEHR
                     AMRASASRWATTTWPKASAVSPSSPLAEPAHS"
     gene            2406..>2723
                     /locus_tag="PLV68_09555"
     CDS             2406..>2723
                     /locus_tag="PLV68_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HRE01535.1"
                     /translation="MTALDQRDISIPFNLLPADGRFGCGPSKVRPEQVTALSAAGTTL
                     LGTSHRQAPVKNLVGSVRAGLADLFALPDGWEVLLGNGGSTAFCDVATFGLVNDRSAH
                     AVFG"
     assembly_gap    2724..2826
                     /estimated_length=103
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2827..3541
                     /locus_tag="PLV68_09560"
     CDS             <2827..3541
                     /locus_tag="PLV68_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRE01536.1"
                     /translation="DGIDVYALTHNETSTGVMMPLVRPAGAAGDALVVVDATSAAGGL
                     LWDPAEVDVYYFAPQKCFASDGGLWFAACSPRALDRIRAIAASGRWVPASLDLKIALD
                     NSVANQTYNTPALATLVMLDAQVQWLLGLGGLAAANARCVASSSALYQWAEARSFTTP
                     FVSDPHKRSEVVGTVDFDGVDANTISAVLRANGVVDTDSYRKLGRNQLRIGMFPAIDP
                     ADVEALTRCIDHVVEALTA"
     gene            3551..>4061
                     /locus_tag="PLV68_09565"
     CDS             3551..>4061
                     /locus_tag="PLV68_09565"
                     /inference="COORDINATES: protein motif:HMM:NF021287.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HRE01537.1"
                     /translation="MRVDDVLSTHIEGLTPLRRPIMVIALTGWFDASGAAVGALDWLL
                     RNRAAPVVATIDPDPFYDFTHERPEVWLDEDRFGHIAWPSNEFRIARFPDDSRDLVVL
                     SGVEPHARWSTYCACITRVAALLGCDAVVTLGANADAVPHTRIPPVVGSSPNDELIRR
                     LGLSRPSYQG"
     assembly_gap    4062..4190
                     /estimated_length=129
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4191..>4408
                     /locus_tag="PLV68_09570"
     CDS             <4191..>4408
                     /locus_tag="PLV68_09570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01538.1"
                     /translation="QHVLGLTIDWAELTAEVERWQSLHDEAVAEDPQATAYVALLERQ
                     FDHRTEATLAAAEDLGTQFEAFLKDLDP"
BASE COUNT          679 a         1503 c         1369 g          615 t
ORIGIN      
        1 acaccagggc gaccagcttg aacgcagccg gcgtgcggtg cacgacggac tcacgagcga
       61 cgtacaactc gctcgcccgc agcgagccat ggctcctgct cacccggtca cgctggcgcg
      121 cctcggcggc gcagcggcct ggtggcggca agcacccctg agatgacgat caggctgagc
      181 gccactccga ccgcacccgc gatcgccaga ctgaccgtct cgttgccgac gccctgggtc
      241 gcgtagtccg ccaagggact cgacgccagc gcatgatcgg tccccgcctg agcgaagccg
      301 tggtcgatcg cgacccgctc caaaccgtcg ggattatcga acgcgaactg gccgatcacg
      361 ctcgcgcaga ccaacgcgac gatgcatcca ccgatcacga acgggcgcag acccaacccg
      421 ccgcgatccg cgctctgctc cgggctcagg tcgtgcgcac ccgcaaccag gtcgggacgt
      481 gacccgagca cggcaccgat cgcgccagcg gtgatcacgc cttcgcccag gccgatcaag
      541 gcatggacac cgaccatcgc cccgaacacc cggtcgaagg gcaccggcgc gctcgccccg
      601 aacaaccact cgatcgagaa cgcgacggct gccaacacga ccgaacacac cgccgccaac
      661 ccggatgnnn nnnnnnncgg ttcggcgcca gccagcggcg gaacagccgg aacagcgccc
      721 acccggcaaa ggcgggtacg acggccatgt tgaccacgtt gtagcccagc gcggtgagcc
      781 caccgtcggc aaagaacaac gcctggatga cgactaccac ggtgacgacg agcaggccga
      841 gccatggccc gagcaagatc gccgccagcg caccgccgag cagatggccg gtggttccgg
      901 cagcgaccgg gaagttgaac atctgggccg cgaagatgaa cgctgcggcc accccggcaa
      961 gcggaatctg acggtcaccg agatggcgac gcgcgaccgc aagcgcggcg gtgacgaagc
     1021 cagccgcgat caccgccgtc gcgacggcca ccggcacctc cagaaagcca tccggtgcat
     1081 gcatcgcaac gagagcgctc atggcgggca cgctagctgt gggctggctc agccagcggc
     1141 gaactcggcg atacggcgga tgccttcggc catgtcgtcg tcgcccatcg cgaagctgaa
     1201 gcgcgcatag cccggtgctc cgaacgcctc acccggcacg aacgccacct tggcttgatc
     1261 gtgtacccca gttccgaagc gtatttgatg tcggccagtt ccagcgtcga gtgggccacg
     1321 tggccgcgca ggtcgcgccc gagcagaccc tgcatgttgg caaagcagta gaacgcgccc
     1381 tgtggctcga tgcaggtgac accgtcgatc gcgttgagca tgccgtgcat cgccttgccc
     1441 cgccgttcga acgcggtgcg catcatgtgc acggcagaca ggtcgccggc cacggcggcc
     1501 agcgccgcct gttgggcgac gttgctgacg ttgctggtgg cgtggctttg gaagttgacc
     1561 gacgccttga tcacgtcgct cggcccgatc atccagccga cgcgccagcc ggtcatggcg
     1621 taggtcttgg cgacgccgtt gacgatgagg cactggtcgg ccaggtcggg taccacggtg
     1681 ggcatcgaca cgaagcggtg cgggccatag gtgaggtgct cgtagatctc gtcggtgacg
     1741 acccacacgc ccttctcgac cgcccagcgg ccgatcgctt cgacctcttc gggcgagtac
     1801 accgcgccgg acgggttgtc gggcgacacg aacagcagca ccttggtgcg gctggtgagc
     1861 gcggcgtcga gctgttcgac ggtgacgcgg aagctggtct cctcggtggt gtcgatcacc
     1921 accggcacac cgcctgcgag cgtgatcgcc tcggggtagg tggtccagta cggcgcgggc
     1981 acgatcacct cgtcgcccgg atcgcacagc gcggcaaagg ccgtgtacac cgcgtgcttg
     2041 ccgccgttgg tgaccagcac ctggttggca gtggtggcaa agccgctgtc gcgcagcgtc
     2101 ttggcggcga tcgcctcacg cagcgcgggc aggcccgcag cgggcgtgta gcggtggttc
     2161 ttggtgtcgc gacacgcagc gacggccgcc tcgacgatgt ggtcgggggt cgggaagtcg
     2221 ggctctccgg cgccgaagcc gatgacgttc tcaccctgag cctgcagcgc cttggccttg
     2281 gcgtcgaccg ccaaggttgc cgactcgttg atcgcagcca gccgctgcga gacgcgccgc
     2341 ggtgtgaccg ctgctgcatg ggaatccggg gaggaagacg taggcatagc tgccatgctg
     2401 gcaccgtgac tgcgctggac caacgtgaca tttcgatccc gttcaatctg ttgcccgccg
     2461 atggccgttt cggctgcggg ccgtccaagg ttcgccccga gcaggtgacg gcactgtccg
     2521 ctgcggggac caccttgctc ggcaccagcc accgccaggc acccgtcaag aacctggtcg
     2581 gctctgtgcg cgccgggctg gccgacctgt tcgcgctgcc cgacgggtgg gaggtgctgc
     2641 tcggcaacgg tggctcgacc gcgttctgcg acgtcgccac cttcgggctc gtcaacgacc
     2701 gcagcgccca cgcggtattc ggcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnncgac ggcatcgacg tgtacgcgct gacccacaac gagacctcga ccggcgtgat
     2881 gatgccgctg gtccgccccg caggtgcggc cggtgacgcc ctggtcgtcg tcgatgccac
     2941 cagcgccgca ggtggcctgt tgtgggaccc ggccgaggtc gacgtctact acttcgcgcc
     3001 gcagaagtgc tttgccagcg acgggggcct gtggttcgcg gcctgctcac cccgcgccct
     3061 cgaccgcatc cgggccatcg ccgcatccgg ccgctgggta cccgcgtcgc tcgatctcaa
     3121 gatcgccctc gacaacagcg tcgccaacca gacctacaac accccggcgc tggccacctt
     3181 ggtcatgctc gacgcccagg tgcagtggtt gctcggcctg ggcgggctgg ccgcggccaa
     3241 cgcccgctgc gtggcctcgt ccagcgcgct gtaccagtgg gccgaggcac ggtcgttcac
     3301 cacgccgttc gtgtccgacc cacacaagcg cagcgaggtg gtcggcaccg tcgacttcga
     3361 cggcgtcgat gccaacacga tctccgcggt gctgcgcgcc aacggcgtcg tcgacaccga
     3421 cagctaccgc aagctcggcc gcaaccagtt gcggatcggc atgttccccg ccatcgaccc
     3481 ggccgacgtc gaagcgctga cccgctgcat cgaccacgtc gtcgaagcgc tgaccgcctg
     3541 agggaccggc atgcgggtcg acgacgtcct gtccacccac atcgagggcc tcacgccgct
     3601 gcggcggccg atcatggtca tcgccctcac cgggtggttc gacgcctccg gcgccgcggt
     3661 cggtgcgctc gactggctgc tgcgcaaccg ggctgcaccg gtggtcgcca ccatcgaccc
     3721 cgacccgttc tacgacttca cccatgaacg acccgaggtg tggctcgacg aggaccggtt
     3781 cggccacatc gcctggccca gcaacgagtt ccgcatcgcc cgctttccgg acgactcccg
     3841 cgatctggtc gtgctgtccg gcgtcgagcc gcacgcgcgc tggagcacct actgcgcctg
     3901 catcacccgc gtcgcggcgc tgctgggttg cgacgcggtc gtcaccttgg gggccaacgc
     3961 tgacgcggta ccccacaccc gcatcccccc ggtggtcggc agttcgccca acgacgagct
     4021 gatccgccgt ctcgggctca gccgcccgag ctaccaaggc annnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gcagcacgtg
     4201 ctcggcctga ccatcgactg ggccgagctc accgccgaag tcgagcggtg gcagtcgctg
     4261 cacgacgagg ccgtggccga ggatcctcag gccacggcct acgtcgcgct gttggagcgc
     4321 caattcgacc accgcaccga ggccaccctg gccgcggcgg aagatctcgg gacccagttc
     4381 gaagccttcc tcaaagacct cgacccga
//
LOCUS       DAORNF010000215         4399 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_14846_length_4399_cov_1.747698, whole genome shotgun sequence.
ACCESSION   DAORNF010000215 DAORNF010000000
VERSION     DAORNF010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4399
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14846_length_4399_cov_1.747698"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>230)
                     /locus_tag="PLV68_09575"
     CDS             complement(<1..>230)
                     /locus_tag="PLV68_09575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01539.1"
                     /translation="TGNVNVIVDVVGYYDDHRHSAADIIDEPGVVYNFKNDATAIGTN
                     TTILSVPIRVPADGYLVMEATANFLPQSVGTD"
     assembly_gap    231..368
                     /estimated_length=138
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<369..680)
                     /locus_tag="PLV68_09580"
     CDS             complement(<369..680)
                     /locus_tag="PLV68_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01540.1"
                     /translation="MSKSPSQHRARWAAIGAAVAVSVGAGGFGIANAIQYDNPKNVYI
                     AITPCRLFDTRAGTDNVGTRSTPLGANETADFTATGSNGNCVLPASMQALQLNVTAVG
                     AT"
     gene            complement(861..>1132)
                     /locus_tag="PLV68_09585"
     CDS             complement(861..>1132)
                     /locus_tag="PLV68_09585"
                     /inference="COORDINATES: protein motif:HMM:NF013625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HRE01541.1"
                     /translation="AATTTTTAPKTGGRNVTDPSDSVRNGDSGEGVKKIQEALVAAGY
                     KLAVDGKFGNVTETAVKDFQKKNKLTVDGVVGPLTWKALEKAANP"
     assembly_gap    1133..1237
                     /estimated_length=105
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1428..2606
                     /locus_tag="PLV68_09590"
     CDS             1428..2606
                     /locus_tag="PLV68_09590"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRE01542.1"
                     /translation="MNTAPASVEPTRDSLLTGPFLVIALVSVCYFASLGMLVATLPKW
                     VEGDLSSSGVVIGIVIGSFAIAAAGVRPWAGQLGDRYGRRMLVAGGSAVVAVSIAGYA
                     LVDNVVLLIVLRLFTGIGEAGVFVGAATAVQDLAPPHRRGEAASYFSVSLYAGTAVGP
                     ILGERIADAHGYDATWIVAACLSALAALFAMRIPVGTKTTIRPKRLLHPAALRPGLVM
                     LLGMMPSVAFSAFLAVYAKSLGIGGVGSVFAVLAVSVLVIRVVGARLPDQLGWRRAST
                     VALLAGMTSSIVMVVWAAKPALYVAAVILAIGQSLLFPALFTAVINEAGEDERSHATG
                     TFSLFFDLAAGIGAPLIGLVVALSSHRGAYVTAAVLAALGFVAQHQLRRSESAGQKIV
                     "
     gene            complement(2591..3352)
                     /locus_tag="PLV68_09595"
     CDS             complement(2591..3352)
                     /locus_tag="PLV68_09595"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRE01543.1"
                     /translation="MGQLDGRTIVITGGNGGIGLGMAQAVGAAGARVVIWGRNADKNA
                     DALATLASAGVDAHALICDVASEDQVESSFAASVDWAGGRIDSLVANAGRGGTRRSFV
                     DTSLDQWRAVMATNLDGVFLCFRAAARHMIEGGHGGALVAVSSTSAIHGAANNEAYGT
                     SKTALLGMVRGLAVALARHHIRVNALLPGWTLTDLAAAGYADDKFRNATVSRTPVRRW
                     ADPSEMGPAAVFLTDPALTFHTGDTMTVDGGYTIF"
     gene            complement(3399..4034)
                     /gene="purN"
                     /locus_tag="PLV68_09600"
     CDS             complement(3399..4034)
                     /gene="purN"
                     /locus_tag="PLV68_09600"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HRE01544.1"
                     /translation="MIDGGASAGQTSSARRVVVLASGSGSNFQALADAGREGTLGGAV
                     VAVVCDQPDAFVIQRGATCGVATDVVPRDPDETRHDYDARLADIVAAHRPDLIVLAGW
                     MRLLSMEFLGRFPGQVINLHPALPGEFPGTHAIERAFRASREGTTTRTGVMVHYVPDE
                     GIDDGPVLATAEVPILANDTIDSLGERIHDTEHHLLVRAVRHALTGRCKPD"
     gene            complement(4031..>4399)
                     /locus_tag="PLV68_09605"
     CDS             complement(4031..>4399)
                     /locus_tag="PLV68_09605"
                     /inference="COORDINATES: protein motif:HMM:NF014788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HRE01545.1"
                     /translation="PHRSYLPVLQRALADHGLIKGLAHITGGGLIDNLPRILPAGCNA
                     VIERGSWPMPPLFRLVESVATALPADELYRTLNMGIGMVVVVASDRTEQLRASIDEET
                     WVIGHLTSNLTGGDRRVIMR"
BASE COUNT          593 a         1331 c         1492 g          740 t
ORIGIN      
        1 ggtcggtgcc gacgctctgt ggcaggaagt tggcggtcgc ttccatgacg aggtaaccgt
       61 cggcgggaac gcggatgggc acggagagga tcgtggtgtt ggtgccgatc gctgtggcgt
      121 cattcttgaa gttgtagacg acgccgggtt cgtcgatgat gtcggcggcg ctgtggcggt
      181 ggtcgtcgta gtagccgacg acgtcgacga tcacattgac gttgccggtc nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnngg tcgcgccgac ggcggtgacg ttgagttgca gcgcttgcat gctggccggc
      421 agcacgcagt tgccgttgga gccggtcgcg gtgaagtccg cggtctcgtt cgcgccgagc
      481 ggcgttgacc gggtgccgac gttgtcggtg ccggctcgag tgtcgaacaa gcggcagggg
      541 gtgatggcga tgtagacatt cttggggttg tcgtattgga tcgcgttggc gatgccgaat
      601 ccgccagcac cgacgctgac ggccaccgcg gcaccgatgg ctgcccaccg agcacgatgt
      661 tgggacgggg acttggacat actctgcctc cacaggaact ggccggacgg aatcgttgag
      721 cggtcggaaa ggcccccaac gccgacagaa cacatcatgc acccaacgga gagacctctg
      781 tgaccaaatg cagtcccgaa caagtaccac cctcggccgt cgaggtatcg acgatgagcg
      841 gaccgctcag cgcaggtcgc tcaggggttg gcggcctttt cgagggcctt ccaggtgagc
      901 gggccgacga cgccgtcgac cgtgagcttg ttcttcttct ggaagtcctt gacggcggtc
      961 tcggtgacgt tgccgaactt gccgtcgacg gccagcttgt agccagcggc gacgagcgct
     1021 tcttggatct tcttgacacc ctcgcccgag tcgccgttcc tgacgctgtc ggatgggtcg
     1081 gtgacgttgc ggccacctgt cttgggcgcg gtggtcgtgg tggtggccgc cgnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntgt cgctgccgca ggcagccacc
     1261 acgagcgcgc acgacgcagc gaacagggcg agggttccgg accgtctcat gggccaaccc
     1321 tagccactct gcgcgcgctc tgtgtcccgc gtcacccaag acagcatccg gcgcgctcga
     1381 cctggacgtc ggtgcacccg tgccgactgg ttagcgtggc caccaccgtg aacacagcgc
     1441 cagcctccgt cgagccgacc cgcgacagtc tgctgaccgg cccatttctg gtgatcgcgc
     1501 tggtgtccgt gtgctacttc gcctcgctcg gcatgttggt ggcaacgctg ccgaagtggg
     1561 tggaaggcga cctcagcagc agtggcgtgg tgatcggcat cgtgatcgga tcgttcgcga
     1621 tcgccgccgc tggcgtgcgc ccgtgggctg gtcaactcgg cgaccggtac ggacgacgga
     1681 tgctggtcgc cggtggatca gcggtggtcg cggtgagcat cgccggctat gcactggtcg
     1741 acaacgtggt gctgctgatc gtgctgcgac tgttcaccgg catcggcgag gcgggggtgt
     1801 tcgtcggggc ggcgacggcc gtgcaagacc tggcgccgcc tcatcgccgg ggcgaggccg
     1861 cgtcgtactt ctcggtgtcg ctctatgcgg gcacggcggt ggggccgatc ctcggcgagc
     1921 ggatcgccga tgcccacggc tacgacgcga cgtggatcgt cgccgcttgc ttgtccgccc
     1981 tggctgcgct gttcgcgatg cgtatccccg tcgggaccaa gacgacgatc cggccgaagc
     2041 ggctgttgca tccagccgcg ttgcggcccg gcttggtgat gctgttgggg atgatgccca
     2101 gcgtcgcgtt ctccgcgttt ctcgcggtgt atgccaagtc gctcggtatc ggtggggtcg
     2161 gctcggtgtt cgcggtgctg gccgtgtcgg tgttggtgat ccgcgtcgtc ggtgcccgct
     2221 tgcccgacca actcggatgg cggcgggcgt cgaccgtggc gctcttggca ggcatgacgt
     2281 cgtcgatcgt gatggtggtg tgggccgcca agccggcact gtacgtcgcc gcagtgatcc
     2341 tggccatcgg tcaatcgctg ctgttcccgg cactcttcac cgccgtgatc aacgaggccg
     2401 gcgaggacga gcgcagccat gcgaccggca cgttctcgct gttcttcgat cttgccgcgg
     2461 gcatcggcgc cccgctgatc ggcttggtcg tggcgctgtc gtcgcaccgt ggcgcctacg
     2521 tcaccgcggc ggtgctggcc gcgcttggct tcgtcgctca gcaccaactg cgtcgcagcg
     2581 aatccgcggg tcagaagatc gtgtagccgc cgtcgacggt catcgtgtcg ccggtgtgga
     2641 acgtgagcgc cgggtcggtg aggaacacgg cggccgggcc catctccgac gggtcggccc
     2701 agcggcgcac cggcgtacga ctgaccgttg cgttgcggaa cttgtcgtcg gcgtagcccg
     2761 cggcggcaag gtcggtcagc gtccagccgg gcagcagcgc gttgacccgg atgtggtggc
     2821 gggccaacgc cactgcgagc ccgcgcacca tgccgaggag agcggtcttc gacgtgccgt
     2881 aggcctcgtt gttggccgcg ccgtggatcg cgctggtcga cgacacggcc accaacgctc
     2941 cgccgtgacc gccctcgatc atgtgccggg cggcggcgcg gaagcacagg aagaccccgt
     3001 cgaggttggt ggccatcacg gctcgccact ggtcgagcga cgtgtcgacg aagctgcgcc
     3061 tggttccgcc gcgaccggcg ttggcgacga gtgagtcgat ccgcccaccg gcccagtcga
     3121 ccgatgcggc aaacgacgac tcgacctggt cttcggatgc cacatcgcag atcagggcgt
     3181 gagcatcgac ccctgcggac gcgagggtgg ccaatgcgtc ggcgttcttg tcggcgttac
     3241 gaccccagat gaccactcgt gcccctgcgg caccgaccgc ctgggccatg cccaagccaa
     3301 tgccaccgtt gccgccggtg atcacgatgg tgcgtccgtc gagttggccc actgggtcct
     3361 ccgtctcgtg ccgcggctgg gtgccgcgag cgtcgccgtc agtctggctt gcagcggccg
     3421 gtcagggcat ggcggaccgc gcgcaccaag agatgatgct cggtgtcgtg gatgcgctcg
     3481 cccaacgagt cgatcgtgtc gtttgcaagg atcggtactt cggcggtggc gagcacgggt
     3541 ccgtcgtcga tgccttcgtc gggcacgtag tgcaccatga ccccggtgcg ggtggtggtg
     3601 ccttcgcggc tggcgcggaa ggcacgctcg atcgcgtgcg tgccggggaa ctctccgggc
     3661 agcgccggat ggaggttgat cacctggccg gggaagcggc cgaggaactc catcgacagc
     3721 aaccgcatcc aacccgcgag cacgatcagg tcggggcggt gggcggccac gatgtctgcc
     3781 agacgggcgt cgtagtcgtg gcgggtctcg tcggggtcgc gaggcacgac gtcggtcgcg
     3841 accccgcagg tggcgccgcg ttggatcacg aacgcgtcgg gctggtcgca cacgacggcg
     3901 acgaccgcgc cgcccagcgt gccctcgcgc ccggcatcgg ccagagcctg gaagttcgac
     3961 ccgctgccgc tcgccagcac gacgacacgt cgtgccgatg acgtctgccc ggcggatgca
     4021 cccccgtcaa tcatcgcatg atcacccggc gatcgccgcc agtgagattg ctggtgaggt
     4081 ggccgatcac ccacgtctct tcgtcgatcg aagcgcggag ttgttcggta cggtcgctcg
     4141 ccacgacgac gaccataccg attcccatgt tcaacgtgcg atacagctcg tccgcgggga
     4201 gcgcggtcgc gaccgattcg accaagcgga acaggggagg catcggccac gaaccgcgct
     4261 cgatcaccgc gttgcagccg gcaggcagga tgcgcggcag gttgtcgatc agcccgccac
     4321 cggtgatgtg ggccaggccc ttgatcagcc cgtgatcggc cagcgcgcgc tggagcacgg
     4381 gcagatagct gcggtgcgg
//
LOCUS       DAORNF010000216         4394 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_14865_length_4394_cov_1.648076, whole genome shotgun sequence.
ACCESSION   DAORNF010000216 DAORNF010000000
VERSION     DAORNF010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4394
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14865_length_4394_cov_1.648076"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>588
                     /locus_tag="PLV68_09610"
     CDS             <1..>588
                     /locus_tag="PLV68_09610"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01546.1"
                     /translation="TDADSTPDTDTGRDDGPGLGTDPNDDVTNHNDIGFDNDTDTPLP
                     QDATDEDDNDYEDIDIDVVYDLALVKVVTNVSPVRSTDTVTWAIRVRNQGNVASGAYS
                     VTDTLPTGMVFATASNGGVASGQVVRWTDLPSLAPGEEVTLSVTTTIGTIHTGDYRNW
                     AEISADSAHTYSGGGATVTDADSTPDADTGADDTLP"
     assembly_gap    589..660
                     /estimated_length=72
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <661..1283
                     /locus_tag="PLV68_09615"
     CDS             <661..1283
                     /locus_tag="PLV68_09615"
                     /inference="COORDINATES: protein motif:HMM:TIGR01167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HRE01547.1"
                     /translation="NDDAVVRVVDPQMAVTKTVGAAVANGDGTFTVTYIVTAANTGTI
                     DTVYSLDDQLLLAPDLLINSASASAPDITVSSTWNGRGDTRLVTDQPIAAGVVHVYTV
                     TVVVTTTTSVLDSSSDCVLDAGESGTGTYNRAVLSHEDDVLEATACVPDIDLPHVLPA
                     PTTVPPVPGGLPSTGTDAGGGVLAAVGLLAVGGLLLAIRRRGLRRV"
     assembly_gap    1415..1543
                     /estimated_length=129
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1544..1880)
                     /locus_tag="PLV68_09620"
     CDS             complement(<1544..1880)
                     /locus_tag="PLV68_09620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01548.1"
                     /translation="MRFIPGQTRLYGPNTFSGSVLSMVGFDLGDKGYDPNFSMAIISI
                     EQIELVDADVIFATTLADGTDRASFESLWSNLSAVQAGQQFDIADDVWMTGIGVLGAH
                     RILDDLETWL"
     gene            2211..>2608
                     /locus_tag="PLV68_09625"
     CDS             2211..>2608
                     /locus_tag="PLV68_09625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01549.1"
                     /translation="MTPSVAPTGTTPRAMLASRNDESSTTTRCGLPGTSVSPIACTTT
                     CGSLLMVVVVSGAADVVVVSGAAVVVGAAVVVGAAVVEGASVLGVVATVVVVSAALSL
                     PQPTAARVRPTRAKGTRRTDRVRDVVGLRMG"
     assembly_gap    2609..2747
                     /estimated_length=139
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2748..3353)
                     /locus_tag="PLV68_09630"
     CDS             complement(<2748..3353)
                     /locus_tag="PLV68_09630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HRE01550.1"
                     /translation="MTRIDAREAKLLPSLLNVLVAGVCDGGRLSRGRTYHRQGAVVDL
                     TVTPGTVTAHVQGSRSDAYAVTVSVAAGHATTPASALVPKAREVGFECSCPDWDTPCK
                     HAVAVMCAFAEAVGDDPQLLVTWRQLTDDAAPRTRPGSRARADAALAQPPSARELSPE
                     ARQALDTWLASPALPDPDHADPPAPWPSLLTAVAPLDPPHHD"
     gene            complement(3350..>4394)
                     /locus_tag="PLV68_09635"
     CDS             complement(3350..>4394)
                     /locus_tag="PLV68_09635"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRE01551.1"
                     /translation="DEAQAIKNHLTKAARAVRRLQAASKFALTGTPIENHLGELWSIL
                     DAVNPGLYGSAAQFGHRYAKPIQKEGDAAATAALTTLTQPFVLRRTKADKTLLPELPD
                     KIEQVAWATLTREQTVMYQAVVDQLLANAHAEIGMKRRGLVLAALTRLKQVCNHPAHA
                     AGDGSRLAGRSGKLNRFDELVDDLIEVGEHALVFTQFREMGLLLQRHLRDRLAMDAPF
                     LHGGVSRTGREGMVRRFQAGEGAPLLIVSLKAGGTGLNLTRASQVIHYDRWWNPAVED
                     QATDRAWRLGQDHTVLVHKLVCQGTLEERIAQLIEDKRQLASSVLGGGETWLSELSTD
                     DLRDLVTLGPGAR"
BASE COUNT          608 a         1330 c         1471 g          645 t
ORIGIN      
        1 accgacgccg actcgacgcc cgacaccgac accggccgcg acgacggacc gggcttgggc
       61 acggacccga acgacgacgt caccaaccac aacgacatcg gtttcgacaa cgacacggac
      121 actccgctgc cccaggacgc aaccgacgaa gacgacaacg actacgagga catcgacatc
      181 gacgtcgtct acgacctggc cctggtcaag gtggtgacca acgtctcgcc ggtgcgttcg
      241 accgacaccg tcacatgggc gatccgggtg cgcaaccaag gcaacgtcgc ctcgggcgcc
      301 tactcggtga ccgacaccct gccgaccggg atggtgttcg ccacagccag caacggcggc
      361 gtcgcctccg gacaggtggt ccgctggacc gatctgccca gcctcgcccc tggggaggag
      421 gtgacgctct cggtcacgac gacgatcggc acgatccaca ccggcgacta ccgcaactgg
      481 gccgagatct cggccgactc ggcgcacacc tactccggtg gcggggcgac ggtcaccgac
      541 gccgactcca cccccgacgc cgacacgggc gccgacgaca ccctgccgnn nnnnnnnnnn
      601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      661 acaacgacga cgcggtcgtg cgcgtggtcg acccgcagat ggccgtcacc aagacggtcg
      721 gcgcggcagt ggccaacggt gatggcacct tcaccgtcac ctacatcgtc accgcggcca
      781 acaccggcac gatcgacacc gtctattcgc tggacgacca gttgttgttg gcccccgacc
      841 tgttgatcaa cagcgcctcg gccagcgcgc ccgacatcac cgtgtcctcg acgtggaacg
      901 gccgtggcga cacccgcctg gtcaccgacc agccgatcgc ggccggtgtg gtccacgtct
      961 acaccgtgac cgtggtggtc acgacgacga cgtcggtgct cgacagcagc agtgattgtg
     1021 tgctcgacgc cggtgagtcc ggcaccggca cctacaaccg ggccgtgctg tcgcacgagg
     1081 acgatgtgct cgaagccact gcgtgtgtgc ccgacatcga cctgccccac gtcttgcccg
     1141 cgccgaccac cgtcccgccc gtgcccggtg gactgcccag taccggaacg gacgcgggtg
     1201 gcggagtgct cgctgccgtt gggctgctgg cggtcggcgg tctgctgctg gcgatccggc
     1261 gccggggact gcgccgggtg tgacagtcca ggtcgttgtc gaccgggtga acgatctctg
     1321 gttcccggga ccttgtccgc gtcctggtga tcagccctgc aagtcgaccc gccgtcccgt
     1381 gatcgacttg taaggacggg ggcctggcgg gtccnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncgagcca ggtctcgagg
     1561 tcgtcgagga tgcggtgagc gccgaggaca ccgataccgg tcatccagac gtcgtcggcg
     1621 atgtcgaact gctggcctgc ttgcacagcg gacaggttcg accacagcga ttcgaacgat
     1681 gcccggtccg tgccgtcggc gagcgtggtg gcgaagatga cgtcggcgtc gaccagttcg
     1741 atctgctcga tactgatgat ggccatcgag aagttggggt catagccctt gtcgccgagg
     1801 tcgaagccga ccatcgagag caccgagcca gagaacgtgt tgggcccgta cagccgggtc
     1861 tggccaggga tgaagcgcac gatcgaggcg gtctggcacc gatgtcggcg ccgacctggg
     1921 ctgcgcgggc ttcgaactcg ccgagcagcg tcgcgccctg gtcggagcgg ttgagggcat
     1981 cggcggtgag gcgaacctgt tcggtccagt tggtgcccga cgactcgctg aacacggtcg
     2041 gagcgatctc gctgagctgg ccgtacagcg cttcgtggcg caccttggcc ccgatgatca
     2101 gatcgggctg catcgaggcg accaactcca gattggggtt tgacgtgtcg ccgacgatgg
     2161 tgatgctggc aaggtcgtcg gcgtcgaagt agccgggtag gccgcgcccg gtgacacctt
     2221 cggtcgctcc caccggcacg acaccgaggg cgatgctggc gtcgaggaac gacgagtcga
     2281 gcacgacgac ccgctgcggg ctgcccggca cctcggtctc gcccatcgca tgcaccacca
     2341 cgtgcggctc gttgttgatg gtggtggtgg tctcgggtgc ggctgacgtg gtggtcgttt
     2401 cgggcgcggc cgtcgtggtc ggtgccgcgg tcgtggtggg cgcggcggtg gtcgagggcg
     2461 cgtcggtgct gggggtggtc gccaccgtgg tggtggtgtc ggccgccttg tcgctgccgc
     2521 agcccacggc ggcgagggtc aggccgacga gagcgaaggg gacgaggcgt actgacagag
     2581 tgcgggacgt ggtggggttg cgcatgggnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngtc gtggtgaggc
     2761 ggatcgagcg gcgcgaccgc ggtcagtagg gacggccagg gtgctggggg gtcggcgtgg
     2821 tcagggtcag gcagtgcggg ggaggcgagc caggtgtcga gtgcttggcg ggcttcgggc
     2881 gagagctcgc gagcactggg cggctgggcc agggcggcgt cggcgcgggc gcgcgagccg
     2941 ggacgcgtcc gcggtgccgc gtcgtcggtg agttgacgcc aggtcacgag caactgcggg
     3001 tcgtcaccga ccgcttccgc gaacgcgcac atcaccgcca ccgcgtgctt gcacggcgta
     3061 tcccagtcgg ggcagctgca ctcgaagccg acctcgcggg ccttgggtac cagggcactg
     3121 gccggtgtgg tggcatgtcc ggccgccacg ctgaccgtga cggcgtaagc gtcgctacga
     3181 cttccctgca cgtgggcggt gacggtgccg ggggtgaccg tgagatcgac caccgcgccc
     3241 tggcggtggt aggtgcggcc gcgtgacagg cgcccgccgt cgcacacgcc cgcgaccagc
     3301 acgttgagca acgacggcag cagctttgcc tcgcgggcgt cgatgcgggt catcgcgcac
     3361 ccggcccgag ggtgacgagg tcgcggaggt cgtcggtgga cagctcgctc aaccacgtct
     3421 cgccgccgcc gagcacgctg gatgcgagct ggcgcttgtc ttcgatgagc tgagcgatgc
     3481 gctcttcgag cgtgccctgg cagaccagct tgtgcaccaa cacggtgtgg tcctggccga
     3541 gccgccaggc gcggtcggtg gcctggtctt cgaccgccgg gttccaccac cggtcgtagt
     3601 ggatgacctg gctggcccgg gtgaggttga ggccggtgcc accggccttg agcgacacga
     3661 tcagaagcgg cgcgccctcg cctgcttgga accggcgcac catgccctcg cgcccggtgc
     3721 ggctgacgcc gccgtgcagg aacggggcgt ccatcgccaa ccggtcgcgc aggtggcgtt
     3781 gcaggagcag acccatctcg cggaactggg tgaacaccag cgcgtgttca ccgacctcga
     3841 tcagatcgtc gaccaactcg tcgaagcggt tgagcttgcc cgagcgcccc gccaggcgtg
     3901 agccgtcgcc ggccgcgtgg gccggatggt tgcagacctg cttcagccgg gtgagtgcgg
     3961 ccagcaccaa cccgcggcgc ttcatgccga tctcggcatg cgcgttggcg agcaactggt
     4021 cgaccaccgc ctggtacatg acggtctgct cgcgggtgag cgtggcccac gccacctgtt
     4081 cgatcttgtc gggcagttcg ggtagcagcg tcttgtcggc cttggtacgg cgcagcacga
     4141 acggctgggt cagcgtggtg agcgcggcag tggccgccgc gtcaccctcc ttctggatcg
     4201 gcttggcgta gcggtggccg aactgcgccg cgctgccgta cagaccgggg ttgaccgcat
     4261 cgaggatgga ccacagctcg ccgaggtggt tttcgatcgg cgtgccggtg agggcgaact
     4321 tggacgcggc ctgcagacgc cgcaccgctc tggcggcctt ggtgaggtgg ttcttgatgg
     4381 cctgtgcttc gtcg
//
LOCUS       DAORNF010000217         4388 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_14883_length_4388_cov_1.949458, whole genome shotgun sequence.
ACCESSION   DAORNF010000217 DAORNF010000000
VERSION     DAORNF010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4388
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14883_length_4388_cov_1.949458"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            166..2394
                     /locus_tag="PLV68_09640"
     CDS             166..2394
                     /locus_tag="PLV68_09640"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HRE01552.1"
                     /translation="MVDDDGDNATIRAPRPMGYQPGLDGLRAISVVAVILYHAGFRWM
                     SGGFFGVEVFFVVSGYLITSLLLDEREQHQRVSLRGFWMRRARRLFPALATTLIAVAS
                     WALLFGSDEQRSTLRRDLPWSIFYLNNWGQITGSVPYFASADPPLLRHLWSLAVEEQW
                     YIVWPLVFVGVMAIGAGRSRSRGVLFIMLSVAVMVATALAHGHASNNALYLSTLTRSS
                     GLLLGAGCAFVWRPWRTRRAAGPDADSRWGALIAGRGGDTYGRWLDPIGAGAVTLLVC
                     IMAVSTLGEGYVSQWLMAVVSVLSMVAIGVVVNPAAVGMRAILSAKPLVEIGRRSYGL
                     YLWHWPIFVIGGAYHSGDWVRFGVAMVVTVAVAEVSFRYVETPIRRGAIARWLRDRET
                     AVGADALRRSWITAGGGVAAAALVATLGLAYQSVDDFDAAVGGDDVSFELDDTGGTGV
                     GGSDPAVPDGTSTQSTAVQAATSTSATTDAGNVSDVTSTTGAGGVTDTVATTSPQTTT
                     TVAASTTLPNLPRRVVIVGDSQAHSLAVNLPNGIGSTFTISDGSVEGCGVYESGKVRS
                     ARQGFSRSFTNCGGFAAKWGRAASGAKAEVALVVLGAWDVFDLDLDGRVVAFASAEAD
                     AAYLAGVQDGIDALVGAGAHVALLEVPCMRPQDVKGAGVPALPERGDDARVAHVNDLL
                     RQAAAANPDTTTFVTGPAEWCGDESISTNLGYRWDGVHVYKPGANLIYETIAPALLAI
                     PL"
     gene            complement(2450..3508)
                     /locus_tag="PLV68_09645"
     CDS             complement(2450..3508)
                     /locus_tag="PLV68_09645"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRE01553.1"
                     /translation="MPKPFRTALVTATCALLLVACGSDTPATTNTNAPAATTTTIVAA
                     TSTTEPSSEATTTAPATTPTAPAGETRVIASSFGDVTVPAKPVRVVALDYAAAVMVEY
                     GLTPVGTTELYEEDHSPELWAGLADIPVVGEWTSIDGLNYEAIAAQRPDMIVGLVRAN
                     QADSDELVTLLSPIAPTVLFAANGSQDVLDVTVQIADAVGVGAQAAADRDAFVARAGE
                     LTTMYGEQLASLRFALINAYDETEFVVYTPQSWWGPLFDAIGATMVPLTDKVDDNGVW
                     LSVEQIGQLDEASVVLYGLDGGDPRDAVASVLDSPLYQALPVAAHTFGIANLFPDRYT
                     TARAGLDELAAILDQLAG"
     gene            3564..>4388
                     /locus_tag="PLV68_09650"
     CDS             3564..>4388
                     /locus_tag="PLV68_09650"
                     /inference="COORDINATES: protein motif:HMM:NF019633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-interacting protein"
                     /protein_id="HRE01554.1"
                     /translation="MNMSIETSAAENLSTCVVTSAETVELPIILRRMMVRAVEQITPV
                     VRRVVVGGEQFDRFVVDGAEQPGFESPGVDDHVKLFFAGPDGLVLPTQGDGELDWPED
                     PRPVARSYTVRAVDHHAKTVTIDMVLHGHGFGARWAAAVTVGDEVHLAGPKQWTAFPN
                     APWYLLVGDDTALPAIARWLEALAPDAVVRVVIATDHPGEYPGLVLAGDRTVTWVAPA
                     MRQGEQPLTDAVAALDWPAATPFVWAAGEVGDVGALRRLLRDQRGLDRASYDLAAYW"
BASE COUNT          611 a         1418 c         1618 g          741 t
ORIGIN      
        1 tctgccgagg tcgtggccaa ggccgccgcg ctcgacgcgg cccgagcggc caaggacttt
       61 gccacagcgg atgcgctgcg ggccgagctc cagaccgacg gatggacggt cgaaaccacc
      121 aaggccggta ccaccgtccg gcgctgatcg gtcagactcg cgctcgtggt agacgacgac
      181 ggcgacaacg ccacgatccg cgccccgcgg ccgatgggct atcagcccgg cctcgacggc
      241 ttgcgggcga tcagcgtcgt tgccgtcatc ctctaccacg ccggcttccg ctggatgagc
      301 ggtggcttct tcggcgtcga agtgttcttc gtcgtctctg gctacctgat cacgtcgctg
      361 ctgctcgacg agcgcgaaca gcaccaacgg gtgagcctgc gtgggttctg gatgcgccgc
      421 gcccggcggc tgttcccggc cctggcgacg acgctgatcg ccgtggcgtc gtgggcactg
      481 ctgttcggca gcgacgagca gcgttcgacg ctgcgccgcg acctgccgtg gtcgatcttc
      541 tacctcaaca actggggcca gatcaccggc agtgtgccct acttcgcgtc ggccgatccg
      601 ccgctgctgc gtcacctgtg gtcgttggcg gtggaagagc agtggtacat cgtgtggccg
      661 ctggtgttcg tcggcgtgat ggccatcggc gcgggccggt cgcggagccg aggcgtgctg
      721 ttcatcatgc tgtcggtcgc ggtgatggtg gccaccgcgc tggcccacgg tcacgccagc
      781 aacaacgcgc tgtacctcag caccctcacc cgatcgagcg gtctgttgtt gggcgccgga
      841 tgcgcgttcg tgtggcggcc gtggcgcaca cgccgtgccg ccggcccgga tgccgactcg
      901 cgttgggggg cgctgatcgc ggggcgtggc ggcgacacgt acggccgctg gctcgacccg
      961 atcggggctg gggccgtgac gctgctggtg tgcatcatgg cggtgtcgac cctgggcgag
     1021 gggtacgtgt cgcagtggct gatggcggtg gtctcggtgc tgtcgatggt ggcgatcggc
     1081 gtcgtcgtaa accccgccgc cgtggggatg cgggcgatct tgtcggccaa gccgctggtc
     1141 gagatcggcc gccgctcgta cgggctgtac ctgtggcatt ggccgatctt cgtgatcggc
     1201 ggcgcgtacc acagcggtga ctgggtgcgg ttcggggtgg cgatggtggt cacggttgct
     1261 gtcgccgagg tcagctttcg ctatgtcgag actcccatcc gtcgtggtgc gatcgcccgg
     1321 tggctgcgcg accgggagac ggccgtgggc gccgacgcgc tacggcggtc gtggatcacc
     1381 gcaggcgggg gagtggccgc ggcggccctg gtggccacgc tcggcctggc ctaccagtcg
     1441 gtcgacgact tcgacgcggc ggtcggcggc gacgacgtct cgttcgaact cgacgatacg
     1501 ggtggcacgg gcgtcggcgg ttcggacccg gccgtgccgg acggtacgag cacgcagagc
     1561 accgcggtgc aggcggcgac gtcgacgtca gcgacgaccg atgcgggcaa cgtgagtgac
     1621 gtcaccagca cgacgggtgc cggtggcgtg accgacaccg tggcgacgac gtcgccacag
     1681 acgaccacga cggtcgctgc ctcgaccacg ttgccgaacc tgccccggcg ggtggtgatc
     1741 gtgggtgact cgcaagcgca ctcgctggcg gtcaatttgc ccaacggcat cggttccacg
     1801 ttcacgatca gcgacgggtc ggtcgagggc tgcggcgtct acgagtcggg caaggtccgt
     1861 tccgcccgcc aggggttctc gcgcagcttc accaactgtg gtggcttcgc ggccaagtgg
     1921 ggccgtgctg cgtcgggcgc gaaggccgag gtcgcgttgg tggtgttggg cgcatgggac
     1981 gtgttcgacc tggacctcga cgggcgcgtc gtcgcgttcg ccagcgcgga ggccgacgcg
     2041 gcctacctcg ccggcgtgca ggatggcatc gacgcgttgg tcggcgcagg tgcccacgtg
     2101 gcgttgttgg aggtgccgtg catgcgcccg caggacgtga agggcgccgg cgtgcccgcc
     2161 ctgcccgaac gtggcgacga cgcgcgggtc gcgcacgtca acgacctgct tcgccaggca
     2221 gcggcggcca acccggacac caccacgttc gtcaccggcc cggccgagtg gtgtggcgac
     2281 gagtcgatca gcaccaacct cggctatcgc tgggacggcg tgcacgtcta caagcccggc
     2341 gccaacctga tctacgagac gatcgccccc gccctcctcg ccatcccgct gtaaccgccg
     2401 tcgcagggtc gcaggggtcg cagggtcgca ggggtcgcag gggtcctggt cagccggcga
     2461 gttggtcgag gatcgcagcc aactcgtcca gcccggcacg ggcagtggtg tagcggtcgg
     2521 ggaacaggtt ggcaatgccg aaggtgtgcg cggcaacggg cagtgcctga tacagcgggc
     2581 tatcgagcac gctcgccacc gcgtcacggg ggtcgccccc gtccagcccg tacaacacga
     2641 cagacgcctc gtcgagttgg ccgatctgct cgacgctgag ccacacgccg ttgtcgtcca
     2701 ccttgtcggt cagtggcacc atcgtggcgc cgatcgcgtc gaagagcggc ccccaccacg
     2761 actgcggggt gtagacgacg aactcggtct cgtcgtaggc gttgatcagc gcgaaccgta
     2821 acgaggcgag ctgctcgccg tacatggtgg tcaactcgcc cgctcgggcg acgaaggcgt
     2881 cgcggtcggc tgcggcctgc gcgccgacac cgaccgcgtc ggcgatctgg acggtcacgt
     2941 cgagcacgtc ttgcgagccg ttggcagcga acaacacggt cggggcaatc ggcgacagga
     3001 gcgtgacgag ctcgtcgctg tcggcctggt tggcgcgcac gaggccgacg atcatgtcgg
     3061 gtcgctgggc ggcgatcgcc tcgtagttga gcccgtcgat cgaggtccat tcacccacca
     3121 ccggaatgtc ggccaggcct gcccacaact cgggcgaatg gtcctcttca tagagctcgg
     3181 tcgtgcccac cggggtgagc ccgtattcga ccatcaccgc cgcggcgtag tcgagggcga
     3241 cgacgcgtac aggcttggcg ggaacggtca cgtcgccgaa gctcgatgcg atcactcgcg
     3301 tctcgcccgc tggcgccgtt ggcgtcgtcg cgggtgccgt cgtcgttgct tcggacgatg
     3361 gttcggtcgt gctcgtggcg gcgacgatcg tggtggtcgt cgcggccggc gcgttggtgt
     3421 tggtggtggc cggtgtgtcg ctgccgcagg cgacgagcaa cagggcgcag gttgccgtga
     3481 cgagggcggt gcggaatggc ttgggcatgg cgatcccctt ctggtggata actgcgaacg
     3541 gtgttcgcag ttagggtaac ctaatgaata tgtcgatcga aaccagcgct gcggagaacc
     3601 tgtccacctg cgtcgtgacg agcgcggaga cagtcgagtt gccgatcatc ctccgccgca
     3661 tgatggttcg agcggtcgaa cagatcacgc cggtggtccg ccgggtcgtc gtcggcggcg
     3721 aacagttcga ccggttcgtt gtcgacggtg ccgagcaacc cggcttcgaa tcgccagggg
     3781 tcgacgatca cgtcaagctg ttcttcgccg gtccagacgg cctggtgttg ccgactcagg
     3841 gcgatggtga actcgactgg cccgaggatc cccggccggt cgctcggtcg tacacggtgc
     3901 gcgcggtcga ccatcacgcc aagacggtca cgatcgacat ggtgctgcac ggccacggat
     3961 tcggggcgcg ctgggccgct gcagtcaccg tcggcgacga ggtgcatctc gccgggccga
     4021 agcaatggac agcgtttccg aatgccccct ggtacctgct ggtgggcgac gacaccgcgc
     4081 tgcctgcgat cgcgcgctgg ttggaggcgc tggcgcccga cgcggtcgta cgcgtcgtga
     4141 tcgccaccga tcatccaggc gagtatcccg gactggtgtt ggcaggcgac cgcacggtga
     4201 cgtgggtggc gccagcgatg cgccagggcg agcagccgtt gaccgacgcc gtcgccgcgc
     4261 tcgattggcc agcagcgacg ccgttcgtgt gggctgccgg cgaggtcgga gacgtgggcg
     4321 cgctgcggcg gctgttgcgc gaccagcggg gcctcgaccg ggcttcgtac gacctcgcgg
     4381 cctactgg
//
LOCUS       DAORNF010000218         4385 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_14897_length_4385_cov_1.833256, whole genome shotgun sequence.
ACCESSION   DAORNF010000218 DAORNF010000000
VERSION     DAORNF010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4385
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14897_length_4385_cov_1.833256"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="PLV68_09655"
     CDS             <1..228
                     /locus_tag="PLV68_09655"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF020034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRE01555.1"
                     /translation="RTGYLQRLAVDPAVQGRGLGYSLTLDSLRWMHRRGMVRAIVNTQ
                     DDNIAALSLYQRMGFRIMPSGLAVMTRLLVA"
     gene            252..>671
                     /locus_tag="PLV68_09660"
     CDS             252..>671
                     /locus_tag="PLV68_09660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01556.1"
                     /translation="MFWTARPAGASPRRAIRRAGTAGAIAFVAVMPVALFATPDAASP
                     ARAAAIVQPLTLLQQDFAIAEAGTLTMSFQPPQPLGADDSIVVSAYRNLTSPEAFAKA
                     VDGEYGIVVDTVRLDAAGAEHPAVVISSDGAVTLSLDT"
     assembly_gap    672..796
                     /estimated_length=125
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            804..>2240
                     /locus_tag="PLV68_09665"
     CDS             804..>2240
                     /locus_tag="PLV68_09665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01557.1"
                     /translation="MPSIATTTDPLQVAVAATVTAPPHIPDVAGSTTPLPESVRRQIN
                     ELLQYPSDVPLSVSISPDVVARLDPNMVSELASIATRGEFLSQPLVPLDPAAASAANQ
                     NELFTDLLQAGEDTINTAIGARPTRTVWIEDDPLTTDGVALLRSLGVTYAVISPETYE
                     AVGTEGGYLGAFTMPSLLREIDLGDGIVPAAVTVPGLTRHLLDTAMAPELAALYTAAE
                     LVAWHAWIAADFAPVRGHSLVLINPDGGVLGPDRVSRLVQMATATGVVEFSALSQMFA
                     TAQPQRLDGNRPARITLAERGIEQATTTPNPSIPTNPSAPNRPTPGEQQPLVAPMVNL
                     SARADSIMETLRRQVTIGGMLVDDGGRTARWQSTLQRLMSTSVTDTQVTTTLGSLGAE
                     LTAISSGVRGPDSYTFTMNGQSTNLHPKIENRTDEPLLVLVHFSSSQDKIEFLDNDLL
                     VELPPNDSHEITVRVRARSNGTSTITVQV"
     assembly_gap    2241..2253
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2254..2712
                     /locus_tag="PLV68_09670"
     CDS             <2254..2712
                     /locus_tag="PLV68_09670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01558.1"
                     /translation="PDSVIKAQVQALTGLAQVLTGGAALVLLAWWMRNWRRNRRARLA
                     VAAVNHPTRAQAVSGSTPAILPTDNTGHAGAGITGSSAIVTATPSTTATTTITAPTVI
                     VPGRAARAGRAGDDSDDDGTAVVGTTDSSPSTRSAIPPDAEGGSATVSDS"
     gene            2709..3569
                     /locus_tag="PLV68_09675"
     CDS             2709..3569
                     /locus_tag="PLV68_09675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HRE01559.1"
                     /translation="MTVRIVSDSACDLPQALADELGIRIVPLSIRFGNTEYIDRKDLT
                     TAEFWAKCAESPVLPETAAPPPGLFEQTYRELIAEGATGIVVVNLSAALSATMQSAEL
                     AARSVATPGADSVDPAVAMPIEVIDSQSVTMGLGSIVAATARFAATGASHADVAAYAR
                     DLAGRTKVWGALDTLDNLKKGGRIGGAKALLASVLSIKPIVEVRNGKVEEGGKQRTRK
                     KALAFLIDKMLSYGTVANVAILQADCDDVDEFVAMVKANYSGEVIVGDIGPVVGSHTG
                     KGTIGLAFTS"
     gene            3658..>4385
                     /locus_tag="PLV68_09680"
     CDS             3658..>4385
                     /locus_tag="PLV68_09680"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HRE01560.1"
                     /translation="MPSPTSPPPPPTLLAQRYRLERKYAQGGMAEVWIATDMSLSRQV
                     AIKILKSNLAADPVVAERFRREIITVARLNHPNIVAVHDAIDFDGRQAVVMPFINGKS
                     LRQLLDEQRRLSPDLTMHIGQHVAAALDAAHKNDLVHRDVKPGNILITPDGRVLLTDF
                     GIAKGVSSNEDLTNDNIMMGTAKYLSPEQVRGVKLDGRADLYALGLVMYECLAGRVPF
                     LGETDADTALARLQRDPTSLNQLHL"
BASE COUNT          714 a         1608 c         1294 g          631 t
ORIGIN      
        1 cgcaccggat acctgcaacg cctcgcggtc gacccagccg tgcaaggccg cggcctcggc
       61 tacagcttga ccctcgactc attgcgctgg atgcaccgcc ggggcatggt gcgggccatc
      121 gtcaacaccc aggacgacaa tatcgccgcc ctctcgctgt accagcggat gggctttcgc
      181 atcatgccca gcggcctggc ggtgatgacc cgcctgctgg tcgcgtgacc tgggtggcag
      241 actgacgagg catgttctgg acggcccgcc ccgccggtgc cagcccccgt cgcgccatcc
      301 ggcgtgccgg tacggcaggt gcgatcgcgt tcgtcgccgt catgcccgtc gcactgttcg
      361 ccacccccga cgcggcatca ccggcccgtg ccgccgcgat cgtgcaaccg ctcacgttgc
      421 tccagcagga tttcgcgatc gccgaggcgg gcacgttgac gatgtcgttc cagccgcccc
      481 agccgttggg cgccgacgac agcatcgtcg tcagcgcgta ccgcaacctg acatcacccg
      541 aggcattcgc caaggcggtc gacggcgaat acggcatcgt cgtcgacacc gttcgcctcg
      601 atgcagccgg cgccgagcac ccggcggtcg tgatcagctc ggacggcgcg gtcacgctgt
      661 cgctggacac cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnnnncgac cgcatgccga gcattgccac caccaccgac ccgctgcagg
      841 tcgccgtcgc ggccaccgtc accgccccgc cccacatccc cgacgtggca ggcagcacca
      901 caccgttgcc cgagtcggtg cgccgccaga tcaacgaact gctccagtac ccatccgacg
      961 taccgctcag cgtcagcatc tctcctgacg tcgtcgcccg gctcgatccc aacatggtca
     1021 gcgagttggc atccatcgcc acccgcggcg agttcttgtc gcaaccgctc gtgccactcg
     1081 acccggcggc agcctcggcc gccaaccaga acgagctgtt caccgacctg ctgcaagcgg
     1141 gcgaggacac gatcaacacg gcgatcggcg cccgccccac ccgcacggtg tggatcgagg
     1201 acgacccgtt gaccaccgat ggcgtggcgt tgctgcgttc gttgggtgtc acctacgccg
     1261 tcatctcacc cgagacctac gaggcggtcg gtaccgaggg tggctacctc ggtgcattca
     1321 cgatgccgtc gctgctgcgc gagatcgatc tcggtgacgg gatcgtgccg gccgcggtca
     1381 cggtgcccgg cctcacccgt cacctgctcg acaccgcgat ggcccccgag ctcgccgcgc
     1441 tgtataccgc ggcagagttg gtggcctggc acgcatggat cgcagccgac ttcgctccgg
     1501 tgcgcggcca ttcgctggtg ttgatcaacc ccgacggcgg ggtcctcggc cccgaccgcg
     1561 tcagccgtct ggtgcagatg gccaccgcga cgggcgtggt cgagttcagc gcgctgtcac
     1621 agatgttcgc caccgcccaa ccgcagcggc tcgacggcaa ccgcccggcg cggatcaccc
     1681 tggccgaacg cggtatcgag caagcgacca ccacgccgaa tccgtcgatc ccgacgaatc
     1741 cgtccgctcc caaccgcccg acccctggcg aacagcaacc gttggtcgca ccgatggtca
     1801 acctgtctgc ccgcgccgac tcgatcatgg agaccctgcg gcgccaagtc accatcggcg
     1861 gcatgctcgt cgacgacggc ggccgcacgg cccgctggca gtcgaccctg caacgactga
     1921 tgtccaccag cgtcaccgac acgcaggtca cgaccacgct cggcagcctc ggcgccgagc
     1981 tcacggcgat cagtagcggc gtgcgcggac ccgactcgta cacgttcacc atgaacggtc
     2041 agtccaccaa cctgcatccc aagatcgaga accgcaccga cgaaccgctg ctggtcctgg
     2101 tccacttcag ctcgagccag gacaagatcg agttcctcga caacgacctc ttggtcgagc
     2161 tcccacccaa cgattcgcac gagatcaccg tgcgtgtgcg ggcccgcagc aacggcacct
     2221 ccaccatcac cgtgcaggtc nnnnnnnnnn nnncccgact cggtcatcaa ggcgcaggtc
     2281 caagccctga cagggctggc ccaggtgctg accggtggcg cggcgctggt gttgctcgcg
     2341 tggtggatgc gcaactggcg ccgcaatcgc cgcgcccggc tcgccgtggc ggcggtcaac
     2401 cacccgaccc gcgcgcaggc ggtctccggc tcgacgcccg cgatcctgcc gaccgacaac
     2461 acaggtcacg ccggcgcagg gatcaccggt tccagtgcga tcgtcaccgc cacgccgagc
     2521 acgaccgcga ccaccaccat caccgcgccc acggtgatcg tgccgggccg cgccgcccgc
     2581 gccggccgcg ccggtgacga ctccgacgat gacggcactg cggtggtcgg caccaccgat
     2641 tcgtcaccga gcacccggtc cgccatacct ccagacgcgg agggtggctc ggctaccgtg
     2701 tctgactcgt gaccgtccgc atcgtttccg atagtgcctg cgatcttccc caggcgctcg
     2761 ccgacgaact cggcatccgc atcgtgccgc tgtcgattcg cttcggcaac accgagtaca
     2821 tcgatcgcaa ggatctgacc accgccgagt tctgggccaa gtgcgccgag tcgccggtgc
     2881 tgccagagac cgccgctccc ccgccgggtc tgttcgaaca gacctaccgc gagctgatcg
     2941 ccgaaggcgc cacgggcatc gtcgtcgtca acctcagcgc cgcgttgtcg gcgacgatgc
     3001 agagcgccga gctcgcggcc cgatcggtcg ccacgcccgg cgccgacagt gtcgatccgg
     3061 ccgtcgccat gccgatcgag gtcatcgaca gccagtcggt cacgatgggt ctcggcagca
     3121 tcgtcgctgc caccgcccgg ttcgccgcca ccggcgcgtc gcatgccgac gtcgccgcct
     3181 acgcccgcga cctcgcggga cggaccaagg tgtggggtgc gctcgacacg ctcgacaacc
     3241 tgaagaaggg cggccgcatc ggtggagcca aggcgctgct ggcctcggtg ctgtcgatca
     3301 agccgatcgt cgaggtgcgc aacggcaagg tcgaagaggg tggcaagcag cgcacccgca
     3361 agaaggcact cgccttcctg atcgacaaga tgctcagcta cggcaccgtc gccaacgtcg
     3421 ccatcctcca ggccgactgc gacgatgtcg acgagttcgt cgccatggtc aaggccaact
     3481 actcgggcga ggtgatcgtc ggcgacatcg gccccgtcgt gggcagccac accggcaagg
     3541 gcacgatcgg cctcgccttc acgagttagt ggtcgggacg ccgcggtccg atcccgggtc
     3601 gcaaacccgt atgaccgttg agctacgtcg tgcgccacta gcgttggcac acgatccatg
     3661 ccctcgccga cttctccccc acctccaccg actcttctcg cccagcgcta ccggctggag
     3721 cggaagtacg cgcaaggagg catggccgag gtgtggatcg cgaccgacat gtcgctgtcg
     3781 cggcaagtgg cgatcaagat cctcaagtcc aacctcgccg ccgaccccgt ggtcgccgaa
     3841 cggttccgcc gcgagatcat caccgtcgcc cgcctgaacc accccaacat cgtcgcggtc
     3901 cacgacgcga tcgacttcga cggtcgccag gccgtggtga tgccgttcat caacggcaag
     3961 agcctgcgtc agttgctcga cgagcagcgc cgcctcagcc ccgacctgac gatgcacatc
     4021 ggccaacacg tcgcggccgc gctcgacgcc gcccacaaga acgacctggt ccaccgcgac
     4081 gtcaagccgg gcaacatcct catcacccct gacggacggg tgctgctcac cgacttcggc
     4141 atcgccaaag gtgtcagcag caacgaagac ctcaccaacg acaacatcat gatgggaacg
     4201 gccaagtacc tctcgcccga gcaggtccgc ggcgtcaagc tcgacggccg ggccgacctg
     4261 tacgccctcg gcctcgtgat gtacgagtgc ctcgcggggc gggtgccgtt cctgggtgag
     4321 accgacgctg acactgcgct ggcgcgcctg cagcgcgacc ccaccagcct caaccagctc
     4381 catct
//
LOCUS       DAORNF010000219         4385 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_14898_length_4385_cov_1.828637, whole genome shotgun sequence.
ACCESSION   DAORNF010000219 DAORNF010000000
VERSION     DAORNF010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4385
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14898_length_4385_cov_1.828637"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..581)
                     /locus_tag="PLV68_09685"
     CDS             complement(<1..581)
                     /locus_tag="PLV68_09685"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRE01561.1"
                     /translation="MFLKSLTLKGFKSFADTTVMQLEPGVTVVVGPNGSGKSNVVDAI
                     AWVLGAQAPSAVRSQKMDDVIFAGTAKRPALGRAEVMLTLDNSAGLLPIEFNEVSISR
                     ILFRTGESEYAINGVACRLLDVQELLSDAGVGRQQHVIVSQGQIDAVLNARPEDRRSI
                     IEEAAGVLKYRRRKEKAERRLPSTEGNLTRLQDL"
     assembly_gap    592..696
                     /estimated_length=105
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<697..>1367)
                     /locus_tag="PLV68_09690"
     CDS             complement(<697..>1367)
                     /locus_tag="PLV68_09690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01562.1"
                     /translation="YYPQNSPLQTFNPVRIADSREALGIPAKLASNTEVQFQVAGLNG
                     VPANATSVVLNLTVVDAPSGYLTAYPCGTPPPLASNVNAFEGRAVANLAVVGVGQGGK
                     VCFIGNSPTHLVVDLQGYNGASSDYNAMTPVRVLDTRETTRVQSYAPVEVPLRGKFGV
                     PNQADRVTVNVTAVGAVGDAFVTVYSCGTVPTVSSGNTSVNRIVATLATVDLSAAGSI
                     CVLSN"
     assembly_gap    1368..1377
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1378..>2643)
                     /locus_tag="PLV68_09695"
     CDS             complement(<1378..>2643)
                     /locus_tag="PLV68_09695"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HRE01563.1"
                     /translation="RESTLGNLVADALLDTLDDAQYGAAEIGVVNPGGLRNELFSSPD
                     GVITYAEANGVLPFVNNLWTISLTGAKFKEMLEQQWQQPPPAPDYAENGLFLQLGLSD
                     NVTYTYDPTRPVNDRILTITVDGYPMDMARSYRIGTFSFLAAGGDNFRAFQAGTDVQD
                     TGLIDRDAWIAYLAANSPVAPDFNRQAVRATSAVPTTATAGSPVVLDLNGLNLTSLGS
                     PQNTSVEVFLGGVSLGTTVVTTRGPAVASLPSLPMGAGSTEITMMVPNTVPSGLQPLQ
                     IVASPTNTTVTVPVTVTSPTPSKPYKPLVFTHNAPVAVPSVRLADTRDGIGLPKAQLT
                     PFSEYVLTVAGANGVPLTAKSVAINVTALNSTVDGWVRVYPCGETQPATSTLNQIGGK
                     IVANLAIVDIGDDGTVCIVSNIASDIVVGL"
     assembly_gap    2644..2683
                     /estimated_length=40
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2684..3721)
                     /locus_tag="PLV68_09700"
     CDS             complement(<2684..3721)
                     /locus_tag="PLV68_09700"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRE01564.1"
                     /translation="MTTLATPVHAAPVTLTVLTFNDFHGRINNNTVRFATTIEQLRAA
                     APGGETNTLLISAGDNIGASEFTSSSADDQPTIDVLNALDVAVSAVGNHEFDKGIDFL
                     QNQVIDGVDYNKADFPYLGANVYAKATTNPVLDEYTVVNVAGVRVGIIGAVTVETPSL
                     VSSLGIANVDFGDPVAAVNRVADQLTDGDPDNGEADVIIADYHEGADLIASLAAAKTR
                     VPFAKIVDDTSAKVDIIVNGHTHQPYTFDAPVPSPGSGNRPVIQTGSYGGNIGKIQFD
                     YDADTDTVTAYSQSVVPTASVAPDLNLPRVGAVNAIVAAAQAAAAIIGEVPVASVTAD
                     ITTAWKSGGYTN"
     assembly_gap    4062..4172
                     /estimated_length=111
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4173..4379)
                     /locus_tag="PLV68_09705"
     CDS             complement(<4173..4379)
                     /locus_tag="PLV68_09705"
                     /inference="COORDINATES: protein motif:HMM:NF018533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01565.1"
                     /translation="MRTRRRQMKSLLLDQAVIAGIGNIYADEILHAARVRHDRPSDSL
                     KPAEITRLHRSIGDILTAAIDAGGS"
BASE COUNT          675 a         1276 c         1453 g          715 t
ORIGIN      
        1 agatcctgca ggcgggtcag gttgccttcg gtggagggaa ggcgccgctc ggccttctcc
       61 ttgcgccggc ggtacttgag cacgccggcg gcttcctcga tgatcgaccg acggtcctcc
      121 ggacgggcgt tgagcaccgc atcgatctgt ccctggctga cgatgacgtg ctgctggcgg
      181 ccgacgccgg cgtcgctgag cagctcttgc acgtcgagca ggcggcaggc cacaccgttg
      241 atcgcatact cgctctcgcc ggtgcggaac aggatgcgac tgatgctgac ctcgttgaac
      301 tcgatcggca acaaaccagc cgagttgtcg agggtgagca tcacctcggc ccgtcccagc
      361 gccgggcgct tggcggtgcc ggcgaagatc acgtcgtcca tcttctggct gcgcaccgcg
      421 ctgggcgcct gggcaccgag cacccaggcg atggcatcga cgacgttgga cttgccggaa
      481 ccgttcggcc ccacgaccac cgtgacgccc ggttcgagct gcatcacggt ggtgtcggcg
      541 aaggacttga agcccttgag ggtgagcgat ttcagaaaca cggcacggcg annnnnnnnn
      601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnggtt ggacaagacg cagatcgacc
      721 cggccgcgct gagatcgacc gttgccagcg tggccacgat gcggttgacc gacgtgttgc
      781 cgctggacac cgtgggcacg gtaccgcagg agtagacggt gacgaatgcg tcgccgaccg
      841 caccgacggc ggtgacattg accgtgaccc gatcagcctg gttgggcacg ccgaacttgc
      901 cccgcaacgg cacttcgacc ggcgcgtacg actgaacccg ggtggtctcg cgggtgtcga
      961 gcacacgaac cggggtcatc gcgttgtagt cgctggacgc gccgttgtag ccctgcagat
     1021 cgaccacgag atgcgtcggg ctgttgccga tgaagcacac cttgccgcct tgacccacac
     1081 cgacgacggc caggttggcg acggcgcggc cttcgaaggc gttgacgttg gacgccagcg
     1141 gaggcggcgt gccgcacggg taggccgtga ggtacccgga cggggcgtcg accacggtca
     1201 ggttgagcac caccgacgtg gcattggcgg gaacaccgtt cagccctgcg acttggaact
     1261 gcacctcggt gtttgaggcc aacttggccg ggataccgag cgcctcacgc gagtcggcga
     1321 tgcgcaccgg gttgaacgtc tgcagcggcg agttctgcgg gtagtacnnn nnnnnnngag
     1381 gccgaccacg atgtcggagg cgatgttgga cacgatgcac accgtgccgt cgtcaccgat
     1441 gtcgacgatg gccaggttgg ccacgatctt gccgccgatc tggttcaggg tcgacgttgc
     1501 cggctgcgtc tcaccgcacg gatagaccct gacccatccg tcgacggtgg agttcagcgc
     1561 cgtgacgttg atggcaaccg acttggccgt gagcggcacc ccgttggcac cggccacagt
     1621 gagcacgtat tcggagaacg gcgtgagctg cgccttgggc aacccgatgc cgtcgcgcgt
     1681 gtcggccagg cgaaccgaag ggaccgcgac cggggcgttg tgggtgaaca ccagcggctt
     1741 gtacggcttg gacggcgtcg ggctggtgac cgtgaccggc accgtgaccg tggtgttggt
     1801 gggcgaggcc acgatctgca gcggctgcag ccccgatggg acggtgttgg gcaccatcat
     1861 cgtgatctcg gtcgagccag cacccatggg cagcgagggc agcgatgcga ccgccgggcc
     1921 acgagtggtg accacggtcg tacccaggct gacgccgccg aggaacacct cgaccgaggt
     1981 gttctgcggc gacccgaggc tggtcaggtt gagaccgttc aggtcgagca ccaccggcga
     2041 accggcggta gccgtggtgg gcaccgcact ggttgcgcgc accgcctggc ggttgaagtc
     2101 gggtgcgacc ggcgagttgg ccgccaggta ggcgatccat gcgtcacggt cgatcaggcc
     2161 ggtgtcttgc acatcggtgc ctgcctggaa ggcccggaag ttgtcacccc cagcggcaag
     2221 gaacgagaag gtgccgatgc ggtacgagcg ggccatgtcc attgggtagc cgtcgaccgt
     2281 gatcgtgagg atgcggtcgt tgaccggccg cgtcgggtcg taggtgtagg tgacgttgtc
     2341 gctcaatccc agctgcagga acagtccgtt ctcggcgtag tccggtgcag gcggcggctg
     2401 ctgccactgc tgctcgagca tttccttgaa tttcgcgccg gtgagcgaga tggtccacag
     2461 gttgttgacg aacggcagca caccgttggc ctcggcatac gtgatgacgc catccggcga
     2521 agagaacaac tcgttgcgca gaccgcccgg gttgaccacg ccgatctcgg ccgcgccgta
     2581 ctgcgcgtcg tccaacgtgt cgagcagggc gtcggcgacc agattgccga gcgtcgactc
     2641 acgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngttggtg taaccgccgc
     2701 tcttccacgc ggtggtgatg tcggcggtga ccgacgcgac cggcacctca ccgatgatcg
     2761 ccgccgcggc ctgcgccgcg gcgacgatcg cgttgaccgc gccgacgcgg ggcaggttga
     2821 gatcgggtgc aaccgaggca gtgggcacga ccgactggct gtaagccgtg accgtatcgg
     2881 tgtcggcgtc gtagtcgaac tggatcttgc cgatgttgcc cccgtacgaa ccggtctgga
     2941 tcaccgggcg gttgcccgag ccgggcgatg ggactggcgc atcgaaggta tacggctggt
     3001 gcgtgtgacc gttgacgatg atgtcgacct tggcgctggt gtcgtcgacg atcttggcga
     3061 acggtacgcg ggtcttggcc gccgcgaggc tggcgatgag atcggcacct tcgtgatagt
     3121 cggcgatgat cacgtcggcc tcgccgttgt ccggatcgcc gtcggtcagc tgatcggcca
     3181 cgcggttgac cgcggcaacc ggatcgccga agtcgacgtt ggcgatgccg agcgaactga
     3241 ccagcgatgg tgtctcgacg gtgaccgcac cgatgatgcc gacgcgcacg ccggccacgt
     3301 tgacgacggt gtactcgtcg agcacagggt tcgtggtggc cttggcgtag acattggcac
     3361 ccaaataagg aaagtcggcc ttgttgtagt cgacgccgtc gatcacctgg ttctggagga
     3421 agtcgatgcc cttgtcgaac tcgtggttgc ccacggctga aacggcgacg tcgagcgcgt
     3481 tgagcacgtc gatcgtcggt tggtcgtccg cgctggacga ggtgaactcg gacgcgccga
     3541 tgttgtcacc ggcgctgatc agcagggtgt tggtctcacc gcccggcgcg gccgcacgca
     3601 actgctcgat cgtggtggcg aacctgaccg tgttgttgtt gatgcgaccg tggaagtcgt
     3661 tgaacgtcaa caccgtgagc gtgaccggcg cggcgtgcac gggcgtggcc agcgtggtca
     3721 tggccaccga gcctccggcg agcgacagcg cgacggccgc tacgaccgct cggcgacgag
     3781 atcgatagtg catcggatac ctccctgcaa agggttgggt gccgagtcgg cgcccaacct
     3841 gtctgaaaca gtcccccgcc cccgcgcacc ctagacgcgg taggaagggt tcgtgaggtc
     3901 ggagcttggc agaggggtct gacaccgttg tgagggccac gtgtccgccg gatgaattcg
     3961 tcacgtcctc cgtcggcctc ccaacggatg cgctcaacgt tggcagcggg ggcagaacga
     4021 cgtcgtccgc ccggcaaagg tgaccttctc gatcgtggct cnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncgagccac cggcgtcgat cgccgccgtc
     4201 aagatgtcgc cgatgctgcg atgcagacgg gtgatctcgg ctggcttcag cgagtcgctg
     4261 ggccggtcgt gacgcacccg cgccgcgtgc aggatctcgt cggcatagat gttgccgatg
     4321 ccagctatca ccgcctgatc gagcagcaac gacttcatct gccgccgccg ggtgcgcaac
     4381 agccg
//
LOCUS       DAORNF010000220         4385 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_14899_length_4385_cov_1.785450, whole genome shotgun sequence.
ACCESSION   DAORNF010000220 DAORNF010000000
VERSION     DAORNF010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4385
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14899_length_4385_cov_1.785450"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..806)
                     /gene="yidC"
                     /locus_tag="PLV68_09710"
     CDS             complement(<1..806)
                     /gene="yidC"
                     /locus_tag="PLV68_09710"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HRE01566.1"
                     /translation="MFEGPAWLVAQFYALTHDYAIAIGLIAVVVMALVTPLTLKSTKG
                     MLEMQRLQPEMRRLQAQHRGDRQKLNEEMMKLYQEHKVNPLASCLPLLLQMPVFFIMY
                     RILHGLANRGEDGTFLPKYISKSSELYQSLLGKTEMKSIGLDLSRTPAHFIGESFGKG
                     IIYALLVVLLAALYYVQQKMVASRAAANPGMSATQAKIMQYLPVAFAVFQLFFLTGLV
                     IYYIIQTLIRIGQNAYITRRFYHGDESLGRQAQAAGQRARDEAKANGQDK"
     assembly_gap    822..892
                     /estimated_length=71
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1176..1251
                     /estimated_length=76
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1400..1534)
                     /gene="rpmH"
                     /locus_tag="PLV68_09715"
     CDS             complement(1400..1534)
                     /gene="rpmH"
                     /locus_tag="PLV68_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003014180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HRE01567.1"
                     /translation="MKRTFQPNNLQRAKKHGFRARMSTKAGRAVLKARRAKGRHRLSA
                     "
     gene            1932..>3184
                     /gene="dnaA"
                     /locus_tag="PLV68_09720"
     CDS             1932..>3184
                     /gene="dnaA"
                     /locus_tag="PLV68_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HRE01568.1"
                     /translation="MSDYEQMWTAVAQLLRAQVSEAVWFSTFQDVVAIGGDGDELRVS
                     APNSLIRDRIMSRYLPLVKDALEEIGQAHRDFAVDVQFSAADEVGADFARDLTDTGVN
                     ATTTGASTGQGELDLDRPPSRTGRSAESSPTDMAGLNPRYSFDTFVKGQSNQFALAAA
                     SRVAETPGRSYNPLFIYGAAGLGKTHLLHAIGHYVHRHYQHDIVRYVSTETFMNEYVD
                     AIRSNTTAAFKRRYRDIDVLLIDDIQFIAGKEGLQEEFFHTFNSLHGANKQIVISSDR
                     MPDAIPTLEERLRGRFKWGLITDVQPPDMETRLAILRNKAERDDCRVPPEALEFIAAN
                     ITSNIRELEGALIRLSAYASLNRVPVTTHLAETLLRDLLSNTAPKARTDDELLSEMAV
                     ILGQSVDALRGKSRQRPLVTSRQIA"
     assembly_gap    3185..3194
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3229..3363
                     /locus_tag="PLV68_09725"
     CDS             3229..3363
                     /locus_tag="PLV68_09725"
                     /inference="COORDINATES: protein motif:HMM:NF019903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01569.1"
                     /translation="MARLFGGRDHTTVIHAVEKIQRLMSERKQVYDQVTDLIQRLTHA
                     "
     gene            3599..>3938
                     /locus_tag="PLV68_09730"
     CDS             3599..>3938
                     /locus_tag="PLV68_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HRE01570.1"
                     /translation="MKFRCERDLLAEALTTAGRAATGRTGTLPVLSGLRLELSGDTLS
                     VTGTDLELTIQLEANVAGERDGGVVVPARLAADIVRSLSAGAVEVSAEADEVSISGGR
                     SQFTVRPLSFD"
     assembly_gap    3939..3948
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3949..>4385
                     /locus_tag="PLV68_09735"
     CDS             <3949..>4385
                     /locus_tag="PLV68_09735"
                     /inference="COORDINATES: protein motif:HMM:NF012915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HRE01571.1"
                     /translation="RLNAPAAGAVTLPTEQVGEALRQVVRAASTDEARPVLTGVLLAA
                     EADGLRMVATDSYRLAVRDLPGASVLGTDQKVLVPGRALNELARLVGNAPELTLRLGE
                     REATFEVGAVRLTTRLIEGEFPNYRQLIPASYPNKLTVSREAL"
BASE COUNT          734 a         1412 c         1378 g          694 t
ORIGIN      
        1 tgcttgtcct gcccgttggc cttggcttcg tcacgggccc gctgaccggc cgcttgggcc
       61 tggcggccga gcgactcgtc accgtggtag aagcgacggg tgatgtaggc gttctgaccg
      121 atgcggatca gcgtctggat gatgtagtag atgaccaggc cggtgaggaa gaacagctgg
      181 aacaccgcga acgcgaccgg caggtactgc atgatcttgg cctgcgtggc cgacatgcca
      241 gggttggccg cggcacggga ggcgaccatc ttctgctgga cgtagtacag cgcggccagc
      301 agcaccacca gcagggcgta gatgatgccc ttgccgaacg actcaccgat gaagtgggcc
      361 ggcgtgcgcg acaggtcgag cccgatcgac ttcatctccg tcttgcccag caggctctgg
      421 tacagctcgg acgacttgct gatgtacttc ggcaggaacg tgccgtcctc tccacggttg
      481 gccaacccgt ggagaatgcg atacatgatg aagaacaccg gcatctgcag caggagcggc
      541 agacatgacg ccaacgggtt gaccttgtgc tcttggtaga gcttcatcat ctcttcgttg
      601 agcttttgcc ggtcaccccg gtgctgggct tgcaggcggc gcatctcggg ttgcagccgc
      661 tgcatctcga gcatgccctt ggtgctcttc aaggtcagcg gcgtcacgag ggccatcacg
      721 accacggcga tcaggccgat ggcgatcgcg tagtcgtggg tgagggcgta gaactgggca
      781 acgagccagg cgggaccttc gaacatgagg ccgactcacc cnnnnnnnnn nnnnnnnnnn
      841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngcaccagc
      901 cggcgtacag cgagccacag gccgcgaccc gcgccgtggg tttcgagggc ttcgagggca
      961 tatgacgaac agctgggggt gaaccgacac ggcgacgggc ggccctcggc ggcacgctgg
     1021 taccaacgca ccgcagccat caaccgtccg ttgatacggc tggcgctggc agcggcgtcg
     1081 aagccggcgt gggcgccatc ttggcggcca accgcgccag gtcggcggcc aggtcggcga
     1141 acgaccgttg tgcggcggcc ggacgagcgc cgatcnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nggtccgacg
     1261 gcgcgtccga tcgcaaacgc cacgcggggc ggcgagacga gtggatcgtg aatgaacgtg
     1321 caccacaagg ttccggcatg aacgcgcgag ccgctgcgag ccaaccgctc gaacagcgct
     1381 cgcccgtgca ggcgttgaat caagccgaga gacggtgacg gcccttggcg cggcgagcct
     1441 tcaacacggc gcggccggcc ttggtgctca tccgggcgcg gaagccatgc ttcttggctc
     1501 gctggaggtt gttcggctgg aaggtgcgct tcatcgcgtc tcctgggtcg tcaacggtgc
     1561 cggtcgctgg tcctgatcga caggacgttg cgggccgttc gtgggctggc agttcgtggc
     1621 ctggcgcgcg gaccgccaga gggcggcaaa acgccagatc gctcaaggct agggggacgt
     1681 ccgcgtcgtt tgcaacgagt cgcgcgaacc accgcgctgc tgctcatagc acaccgcacg
     1741 tggccgatcg cgtctttcgg cttgcccgcg acacccgaga attgcgctct gacctggccc
     1801 gacgcgtccc aagttcagcc cgccagaaat cgctgctagc tttccgtgtc ctgccgggtg
     1861 agtcggccgc gttgtacaca gtgtgtggac aatcgtgtgg aaacctcggc ggcggggaga
     1921 aggaggtgca ggtgagcgac tacgaacaga tgtggacagc ggtcgctcag ctgctccggg
     1981 cccaggtgtc cgaggcggtg tggttctcca cattccaaga cgtggtggcc atcggcggcg
     2041 acggcgacga gctgcgggtc agcgctccca acagcctcat ccgcgaccgc atcatgtctc
     2101 gctacctgcc cttggtgaag gacgcgctgg aagagatcgg ccaggcccat cgtgactttg
     2161 ccgtcgacgt gcagttcagc gcggccgacg aggtcggtgc cgacttcgcc cgcgacctca
     2221 ccgacaccgg cgtgaacgcg accaccaccg gagcctcgac cggccagggc gaactcgacc
     2281 tcgaccgccc accctcacgc accgggcgca gcgccgagag ctcacccacc gacatggcgg
     2341 ggctgaaccc gcgctactcg ttcgacacct tcgtcaaggg tcagtccaac cagttcgcgc
     2401 tcgccgcggc gtcgcgggtg gccgagacac cggggcgcag ctacaacccg ctgttcatct
     2461 acggcgcggc cgggttgggc aagacccatc tgctgcacgc gatcggccat tacgtccatc
     2521 gccactacca gcacgacatc gttcgctacg tcagcaccga gacgttcatg aacgagtacg
     2581 tcgacgcgat ccgttcgaac accaccgcgg cgttcaagcg tcgttaccgc gacatcgacg
     2641 tactgctcat cgacgacatc cagttcatcg caggcaaaga gggactgcaa gaggagttct
     2701 tccacacctt caactcgctg cacggggcca acaagcagat cgtcatcagc agcgaccgca
     2761 tgcccgatgc catccccacc ctcgaagagc gcctgcgcgg ccgcttcaag tggggtctga
     2821 tcaccgacgt acagccgccc gacatggaga ctcgactggc gattctgcgc aacaaggccg
     2881 agcgcgacga ctgccgggtg ccacccgagg cgctcgagtt catcgccgcc aacatcacca
     2941 gcaacatccg cgagctcgaa ggtgcactca tccgcctcag cgcgtacgcc agcctcaacc
     3001 gggttccggt caccacccac ctggccgaga cgttgttgcg cgacctgctc agcaacactg
     3061 cgcccaaggc ccgcaccgac gacgagttgc tcagcgagat ggcggtcatc ctcggccaga
     3121 gcgtcgatgc gctgcgcggc aagagccgtc agcggccgtt ggtcaccagc cgccagatcg
     3181 cgatnnnnnn nnnncttccg cgatctcacc gacctcagct atcccgcgat cgcccggctc
     3241 ttcggtggcc gcgaccacac caccgtcatc cacgccgtcg agaagatcca acgcctgatg
     3301 agcgagcgca agcaggtgta cgaccaggtc accgacctca tccagcggtt gacgcacgcg
     3361 tgatgagcac tgtgaacaac agccgtcgcg gctgtggacg cacgctgaac atcgtcggac
     3421 aacctcgcgt tgtccacccc ggttgcaatc gcgtgtcgtt cgcctgggga tcactgtgga
     3481 tgggggtggc gcaacgatca cggctctacg ctgcggcgcg gcgtcgttgt ccacaatcca
     3541 cagcgcttat tagcactact tccttgatac atcacctctt ggtgggaaag gttgccccgt
     3601 gaagttccgc tgtgaacgcg atctgttggc cgaggcgttg accaccgccg gacgtgcggc
     3661 aacgggccgt accggcacct tgccagtcct gtccggtctg cgcctcgagc tctctggcga
     3721 cactctgtcg gtgaccggta ccgatctcga gctcaccatc cagctcgaag cgaacgtggc
     3781 cggcgaacgc gacggcggcg tggtcgtacc agcccggttg gcggccgaca tcgtgcgctc
     3841 gctcagtgct ggggccgtcg aggtgtcggc cgaagccgac gaggtgtcga tcagcggtgg
     3901 ccgttcgcag ttcaccgtgc ggccgttgtc gttcgacgnn nnnnnnnncc cgtctcaacg
     3961 caccggcggc gggggcggtc acgctgccga ccgaacaggt cggcgaggcg ttgcgccaag
     4021 tggtccgtgc ggccagcacc gacgaggccc gacccgtact gaccggcgtg ttgctggcag
     4081 ccgaggccga cggcctgcgc atggtggcta ccgattcgta ccgcttggcc gtgcgcgacc
     4141 tgccgggcgc cagcgtgttg ggcaccgacc agaaggtctt ggtgcccggc cgggcactca
     4201 acgagttggc ccgcctggtc ggcaacgccc ccgagctcac gttgcgcttg ggcgagcgcg
     4261 aagccacctt cgaggtcggc gcggtgcggc tgaccacgcg cctgatcgag ggtgagttcc
     4321 ccaactaccg ccagctcatc ccggccagct accccaacaa gctcacggtg tcgcgggaag
     4381 cgctc
//
LOCUS       DAORNF010000221         4380 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_14924_length_4380_cov_1.727861, whole genome shotgun sequence.
ACCESSION   DAORNF010000221 DAORNF010000000
VERSION     DAORNF010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4380
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14924_length_4380_cov_1.727861"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>463)
                     /locus_tag="PLV68_09740"
     CDS             complement(<1..>463)
                     /locus_tag="PLV68_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HRE01572.1"
                     /translation="LAGVLLSESTFRHQIEADIEPFRGILLGLFFLGAGMALDLAVVA
                     ANWPLIVGAVLAMMVVKANKYADGIGGHLSTFASSASLYDIGFNHFFRGKDDGNAGDH
                     IYIQGHAAPGIYARAYMEGRLTEDDLDHFRREIGRDHRGLSSYPHPRLMPAF"
     assembly_gap    464..498
                     /estimated_length=35
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            653..1168
                     /locus_tag="PLV68_09745"
     CDS             653..1168
                     /locus_tag="PLV68_09745"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H"
                     /protein_id="HRE01573.1"
                     /translation="MTDRATDRVAVYTDGACRGNPGPGGWAWAVAPEGMPRGTGGEAQ
                     STNQRMEIKAALEAMRSLPGPMIVRSDSTYVVNCVNKKWYVGWKRNGWRNSAKQPVAN
                     QDLWRPFFEIYDPQRHTFEWVKGHSGDRMNDMVDALAVAASKGPFDAPPPPPSPRADP
                     PVQPAPAATLF"
     gene            1238..>1654
                     /locus_tag="PLV68_09750"
     CDS             1238..>1654
                     /locus_tag="PLV68_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRE01574.1"
                     /translation="MDLNGVSAIVTGGASGIGAATSRLLASKGAKVVVADLQADKGTA
                     LADEIGGAFCKVDVTNTDDIINAVEMAKSMGPVRVLVNSAGIGWAQRTVGKDGSYESA
                     ANLDAFKKVIAINLIGSFDCIRIAATAMSTTEPLAHG"
     assembly_gap    1655..1698
                     /estimated_length=44
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1699..1990
                     /locus_tag="PLV68_09755"
     CDS             <1699..1990
                     /locus_tag="PLV68_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRE01575.1"
                     /translation="SASKGGVVGMTLPIARDLAAVGIRVNTVAPGLIDTPIYGSGDAS
                     EAFKANLEKGVLFPHRLGDGSELASMVVECITNSYMNAETIRVDGGIRMPPK"
     gene            complement(1997..>2117)
                     /locus_tag="PLV68_09760"
     CDS             complement(1997..>2117)
                     /locus_tag="PLV68_09760"
                     /inference="COORDINATES: protein motif:HMM:NF014065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional ornithine
                     acetyltransferase/N-acetylglutamate synthase"
                     /protein_id="HRE01576.1"
                     /translation="LRGDDVVIHVSLGIASGTATVWGCDLTDGYVRINADYTT"
     assembly_gap    2118..2243
                     /estimated_length=126
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2244..3163)
                     /locus_tag="PLV68_09765"
     CDS             complement(<2244..3163)
                     /locus_tag="PLV68_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ornithine
                     acetyltransferase/N-acetylglutamate synthase"
                     /protein_id="HRE01577.1"
                     /translation="MSATVPTSLPRGFSAYVANIGIKDDTTDFVVVAAATTCPAAGVF
                     TRSRFAGPSVVLSRANIADRTARAVVVISKNANVATGPTGLANAQSVVDGVAAALGAD
                     ADDVLIASTGVIGRQYPMDRVRAGLDTFSDMARGGELPSDDAWAVAGGIMTTDTVAKV
                     ASATVAGARARVVGVAKGVGMIEPDMATLITLVFTDAEVPSAGLDESFRRVVDRTFNC
                     VSVDTDTSTSDTAIILASGAAGPVEPAAFEAALHEVLLSLTKQVARDGEGAETLIEVR
                     VDGARDDAQAKRVAKSIVNSPLVKTAVHGA"
     gene            3402..>3994
                     /locus_tag="PLV68_09770"
     CDS             3402..>3994
                     /locus_tag="PLV68_09770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01578.1"
                     /translation="MSNPYNPHPGGFPAPGAGGGFQPPSAPTSGPVFLPPQQPGGAPL
                     TGVGAETPFTVGGLSEPVVGEPPRRKRSRGKMIGAVVGVAAVLGAGVFAVTKLRSNET
                     SGGASSPEALGTALVDTLNNEDILGAIDLLLPTERDTFRQPLVDLVSELKRLEVFSKD
                     ADLGGVSGLDIDIDVPDVDIEETSAKDVVMVSLDADST"
     assembly_gap    3995..4040
                     /estimated_length=46
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4041..>4380
                     /locus_tag="PLV68_09775"
     CDS             <4041..>4380
                     /locus_tag="PLV68_09775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01579.1"
                     /translation="DAAFDGEQPDFDESEDSPFNPTFAAVERDGRWYISLLYTGAVGA
                     AGIDNVPAREEGIAPVGAKTPEGALDNVIQSLVGLDLESVVAGLNPNEAEVLQRFAPL
                     FIDDMQDELDD"
BASE COUNT          687 a         1358 c         1420 g          664 t
ORIGIN      
        1 agaaggcggg catcaggcgc gggtggggat agctgctgag accacggtgg tcgcggccga
       61 tctcgcggcg gaagtgatcg aggtcgtcct cggtgaggcg gccctccatg tacgcccggg
      121 cgtagatacc gggggcggcg tggccctgga tgtagatgtg gtcgcccgcg ttgccgtcgt
      181 ccttgccgcg gaagaagtgg ttgaagccga tgtcgtacag cgacgcgctg ctggcgaagg
      241 tggacaggtg gccaccgatg ccgtcggcgt atttgttggc cttgaccacc atcatcgcca
      301 gcaccgcgcc gacgatcagc ggccagttgg cggccaccac cgccaggtcc agggccatgc
      361 cggcgcccaa aaagaacagg cccagcagga tgccgcggaa cggctcgatg tcggcttcga
      421 tctggtgccg gaaggtcgat tcggacagca gcacgccggc cagnnnnnnn nnnnnnnnnn
      481 nnnnnnnnnn nnnnnnnntc ctgcgtctcg atcggatcgg tgtcgggcaa ctgatggacg
      541 aagccgtcga agatcatggg tccagtctgg cagggttacc ggagggtagt gccgaacacg
      601 tttcgatgtg ctatgccgcg gacggccggt cggcccggcc acactggcac ccgtgactga
      661 tcgagcaact gatcgagtag cggtgtacac cgacggcgcc tgccggggga atcctggccc
      721 cggcggttgg gcatgggcgg tggcgcccga agggatgcct cgcggcaccg gtggcgaggc
      781 ccagtcgacc aaccaacgga tggagatcaa ggccgcgctc gaagcgatgc ggtcgctgcc
      841 cggaccgatg atcgtccgat ccgactcgac ctatgtggtc aactgcgtca acaagaagtg
      901 gtacgtcggt tggaagcgca acgggtggcg caactcggcc aagcagccgg tggccaacca
      961 agacctctgg cggccgttct tcgagatcta cgacccccag cggcacacct tcgaatgggt
     1021 caagggtcac agcggcgacc gcatgaacga catggtcgac gcccttgccg tcgccgccag
     1081 caaggggccg ttcgatgcgc caccgccacc gccatcgccg cgtgccgatc cgcccgtcca
     1141 acccgcccca gcggcgacac tgttttgaga cggataccgc tctgagacgg cggggccgaa
     1201 tcgtcggcgc cgtgggcggt tgtctagttt gcgacacatg gatctgaacg gagtcagtgc
     1261 aatcgtcacg ggtggggcat cgggcatcgg tgcggccacc tcacgccttc tcgcctcgaa
     1321 gggtgccaag gtcgtcgtgg ccgacctgca agcagacaag gggaccgcgc tcgccgacga
     1381 gatcggcggc gcgttctgca aggtcgacgt caccaacacc gacgacatca tcaacgcggt
     1441 cgagatggcc aagagcatgg gtccggtgcg ggtgctggtc aactcggccg gcatcggctg
     1501 ggcgcagcgc accgtcggca aggacggcag ctacgagtcg gcggccaacc tcgacgcctt
     1561 caagaaggtc atcgccatca acctgatcgg cagcttcgac tgcatccgca tcgccgccac
     1621 ggcgatgagt accaccgagc cgctggccca cggcnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnca gcgcatccaa gggcggtgtc gtcggcatga cgctgccgat
     1741 cgcccgcgac ctcgctgcgg tcggcatccg cgtcaacacc gtggcgcccg gtctgatcga
     1801 caccccgatc tacggcagcg gcgatgccag cgaggcgttc aaggccaacc tcgaaaaggg
     1861 tgtgctgttc ccgcatcgct tgggcgacgg cagcgagctg gcgtcgatgg tggtcgagtg
     1921 catcaccaac agctacatga acgccgagac catccgggtc gacggcggca tccgcatgcc
     1981 accgaagtga aaccgatcac gttgtgtagt cggcgttgat gcggacgtag ccgtcggtca
     2041 ggtcgcagcc ccacaccgtg gctgtgccgc tggcgatgcc gaggctgacg tggatcacca
     2101 cgtcgtctcc gcgcaggnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnntcggcgc cgtgcacggc cgtcttcacc aggggtgaat
     2281 tgacgatcga cttcgcgacc cgcttggcct gggcgtcatc gcgggcaccg tcgacgcgga
     2341 cctcgatcag cgtctcggcc ccttcgccgt cgcgggcgac ctgcttggtc aacgacagca
     2401 gcacctcgtg caacgcggct tcgaacgcgg ccggctcgac cggtccggcc gcaccgctgg
     2461 cgaggatgat cgccgtgtcg ctggtggagg tgtcggtgtc gaccgacacg cagttgaagg
     2521 tgcgatcgac gacccggcgg aagctctcgt cgagcccggc gctgggcacc tcggcgtcgg
     2581 tgaacaccag cgtgatcagt gtggccatgt ccggttcgat catgccgacg cccttggcga
     2641 cgccgacgac ccgtgccctg gccccggcca cggttgcgct ggccaccttg gcaaccgtgt
     2701 cggtcgtcat gatcccgccc gcgacggccc aggcatcgtc gctgggcagc tcaccacccc
     2761 tggccatgtc ggagaacgtg tccagtccgg cgcgcactcg gtccatcggg tactggcgcc
     2821 cgatcacccc cgtcgacgcg atcaacacgt cgtcggcgtc ggcaccgagc gcggcggcga
     2881 caccgtcgac cacggactga gcgttggcca gcccggttgg ccccgtcgcg acgttggcat
     2941 tcttggagat caccacgacc gcccgcgccg tccggtcggc gatgttggcg cggctgagca
     3001 ccacgctggg tccggcaaag cggctgcggg tgaacacccc tgccgcgggg caggtcgtag
     3061 cggcggcgac gacgacgaag tcggtcgtgt cgtccttgat accgatgttg gcgacgtagg
     3121 ccgagaaccc gcgagggagc gatgtgggga cggtggcgga catgatcggc agggtatatc
     3181 tcgggacacc gatcgaccgc cacgggttcg cagtgtgatg gttgcgtcgc gtcgtggccc
     3241 gccttggcga ccgtgtcctg gcgacggtgt tgtcctgctg ttgcgaaaga gatgagacga
     3301 tcttcacgtt ctgcgcggcc cctctcgcgg gacgggcctc tacacttcgc gcaccgccgg
     3361 atgtcgcgcg ccgacatctc gacgtaggag gagcaggaca catgagcaat ccgtacaacc
     3421 cccatcccgg tggctttccc gcccctggcg ccgggggcgg cttccagccg ccgagcgctc
     3481 caacatctgg ccctgtcttt ctgccgccgc agcagccggg cggtgcgccg ctgactggtg
     3541 ttggcgcaga aacgccattc accgtcggtg ggttgtccga gccggtcgtc ggtgagccac
     3601 cgcggcgcaa gcgcagccgg ggcaagatga tcggtgcggt ggtcggtgtc gctgcggtgc
     3661 tcggtgccgg ggtgttcgcc gtgaccaagt tgcgcagcaa cgagacgtcc ggtggggcat
     3721 catcgcccga ggcgttgggc acggccctgg tcgacacgct gaacaacgag gacatcctcg
     3781 gtgcgatcga cctgctgctg cctaccgagc gggacacgtt ccgccagccg ttggtcgatc
     3841 tggtcagcga gctgaagcgg ttggaggtgt tcagcaagga cgccgacctc ggcggcgtgt
     3901 ccggtctgga catcgacatc gacgtgcccg atgtcgacat cgaagagacc agcgccaagg
     3961 atgtggtgat ggtcagcctc gacgccgact cgacnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn gatgccgcct tcgacggcga acaacccgac ttcgacgaga
     4081 gcgaggactc gccgttcaac cccaccttcg ccgctgtcga gcgtgacggc cggtggtaca
     4141 tcagcctgct gtacacgggt gcagtcggcg ccgcgggcat cgacaacgtt cccgcccgcg
     4201 aggaaggcat cgcgccggtc ggtgccaaga ctcctgaagg tgccctcgac aacgtgattc
     4261 agtcgctggt cggcctcgac ctggagagcg tcgtcgccgg tctcaacccc aacgaggccg
     4321 aggtgctgca gcgcttcgcc ccgctgttca tcgacgacat gcaggacgaa ctcgacgacg
//
LOCUS       DAORNF010000222         4374 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_14951_length_4374_cov_1.951146, whole genome shotgun sequence.
ACCESSION   DAORNF010000222 DAORNF010000000
VERSION     DAORNF010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4374
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14951_length_4374_cov_1.951146"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            636..3114
                     /locus_tag="PLV68_09780"
                     /pseudo
     CDS             636..3114
                     /locus_tag="PLV68_09780"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
     assembly_gap    3115..3230
                     /estimated_length=116
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3445..4128)
                     /locus_tag="PLV68_09785"
     CDS             complement(3445..4128)
                     /locus_tag="PLV68_09785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01580.1"
                     /translation="MTRTPKPTKTLKPTRTPKPSATLTPTVTPVPIAVLKTWPVGAWV
                     ELDATVIVPPGVLGARVLVVQDATGSIPVYLGRGAWPPGVIPGNGFHALGLSRLRGGV
                     LEFYVRLLPQASFSPPGAVPAPVPITTGGAAQHPYEVVTVTGRVTRLESSALWIDDGS
                     GVVRIPFGAANGLKRPQVSRGETITLTGLVIVQSATTTRSAGWQIQPRGPADVGTLAI
                     LRPTRTPKP"
     gene            4118..>4374
                     /locus_tag="PLV68_09790"
     CDS             4118..>4374
                     /locus_tag="PLV68_09790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01581.1"
                     /translation="MRVIDGVLVGLGVRVIDGVFVGLGVRVGLGVRVIDGVFVGLGVR
                     VIDGVFVGLGVRVIDGVLVGLGVRVIDGVFVGLGVRVGLGVR"
BASE COUNT          670 a         1493 c         1405 g          690 t
ORIGIN      
        1 gcttccgcac tttccacggt gatgtcaccg gcgttcagct gcgtgtctac gacgtcgaca
       61 cggccgcgca gaccctcctg cccatgaccc cggcggcgac cgatgtaggc tgctatcagg
      121 ccgggcttga ggatcgcacg tgcgacttct gggccgtgac cctgcccaac gactcgcccg
      181 ccaacgtctg gtaccgcttc gtcgtggccg acggcaccga cgtcgactac tacggtgaca
      241 acacgacggc gctcgacggc ggcctcggcg cgccgtcgga cgacccgatc gacaacagct
      301 acgccctgat ggtccacgat ccggccttca caagcccgtc ctgggcccag gacgccgtga
      361 tctaccagat cttcccggat cgcttccgca atggggaccc gcgcaacgat ccggacaccg
      421 gcgacccgcg ctacgacgac ccgatgatcg cgctggattg gggcacgttg cccgagggct
      481 actgccgcaa ctacgccgat cgcgacgaga attgcccgtg gcgcttcgac gacacacccc
      541 cggacaccag cccgacggtc gagagcccgc gcggccgcga ctactacggc ggcgacctgc
      601 gcggtgtgtt gcagcgcctg ccctatctgc gcagcatggg cgtgaccgtg atctacttca
      661 acccgatctt cacggcccgc tcgaatcatc gctacgacac cgccgactac tacgagatcg
      721 gcgctcggca cgctgaatga ctggaaggcg ctcgagcgcg ccgcccggtt gctcggcatg
      781 cgcatcgtgg tcgatggcgt gttcaatcac ctctcgtccg acagtgcgtt cttcgatcgc
      841 tatcaggcct acgacatcgt cggtgcctgc gagtcggccg gctccgagtt ccggccgtgg
      901 ttcacgttcc ggccgccgtc ggccagcgaa cccgcgccct gcgtgccgtc gacgcccggc
      961 ggggccgaca cctactactc cggctggttc ggcttcgaca gcatcccggt gattaacaag
     1021 agcctgcccg aggtggtcga ctacttcgtc acaggcgacg acagcgtctc gaagtactgg
     1081 ctggagcagg gggccgacgg ctggcgcctc gacgtgatgg gcgacgcctc gttcccggcc
     1141 ggatactggg aggagttccg ctcggtcgtc aaagcggaga gcgccgacgc ggtcatcatc
     1201 ggcgagctgt ggcagaaaga cagcacgctg ctgcgcttcc tgcgcggcga tcgtgccgat
     1261 accaccatga actaccggtt gcgcgacgcg gtgctggggc tgctcacgcc caacgccagc
     1321 ttcgacggca agggctttgg cgacagcggc cggcagatca cgccgaccga gttcgccaac
     1381 cgcatcctgg cgatccgtga ggattacccc gacgcggcgt actacagcct gatgaacctg
     1441 ctcgacagcc atgacaccga gcggctgttg tggaccctga cggccggggc cgagacccag
     1501 gccgagcgcg agttcaacgc cgccaacctg gccgcgggca agcagcgtgt caagctggcc
     1561 tcgctgatcc agttcaccct gcccggcgcg ccgaccgtgt actacggcga cgaggtcggt
     1621 gtaaccggcg acgacgaccc tgacgatcgt cgcacctatc cgtgggccga taagggcggc
     1681 aagcccgaca cgcagctctt cgcccactat gcctcgcttg gctggctccg cgagttcttc
     1741 ccggcgctgg ccaccggtga cctgcgcatg ctgctggtcg acgatgccaa caacacgctg
     1801 gcctacggcc ggcagctcaa caaccagggc gccgtgatcg tgctcaaccg cggcgacgtc
     1861 gagcagacca tcacggtgcc ggtcgccggg ttcctgcctg agcgcacccg gctgacccag
     1921 gtgtacagcc tgagcggcgg attgctgcgc cagtatcgcg tcagcagcgg cgccctgtcg
     1981 gtgaccgtgc cggcgcttgg cggcatgatc ctcgtgacga acggcgctga cctgacgccc
     2041 accgctgccc cgaccgggct gacggcgacg gccagcacaa gccaggtgaa cctcgcctgg
     2101 aacgccgtgc cgcgcgcggg cggctataac gtctaccgca gcccgctgac gggcggcggc
     2161 ttcgtcaaac tcaacggtgc gccgctgacc ggcctgacct ataccgacac cggtctgcgc
     2221 aacgggcgcg attactacta cgtcgtccgc gcgcttgata gcgtgggcaa tgagagcgcg
     2281 gcctcgcccg aggccagtgc catgccgacc tacaccatcg gctgggctaa cctccagtgg
     2341 cctccgtcgg tcgatcacac agtcagcgtg gtcaaccgca cgccggactt ctatgggcag
     2401 gtctggatcg atggcgtgac gaatcagccc ggcgtcgccg acacgctgat cgccgaggtc
     2461 ggctacggcc cggtcggcag cgatccggcg ggagccggct ggctctggtc gccggctgtg
     2521 ttcaacatcg acaacgggaa caacgacgag ttcaagggca gtttcctgcc cgacatcgtg
     2581 ggcagctacg acgtcgtata ccgctactcg acctcggccg ggcgcagctg gctatacgcc
     2641 gacttgggcg gtccggtggc cgcaggcgcg ctgccggctc agcccgggcg tttgaacgtg
     2701 ctcgcagacg cggataccgt gccaccggcg gcgccgaccg ggttgcgagt cgactcggcc
     2761 tcaccggccg ggatcgtgct cgcctgggat gcggtggtcg gcgacgcaag cctgtatggc
     2821 tacgaggtgc gccgcagttc ggccggcggc gcttccgaga ccgtcgcgct cgtcacggcg
     2881 gcggcgttca ccgacctcga cgtccaacag ggcgtggcgt acacctacac cgtgcgcgcc
     2941 gtggacctcg cgtacaaccg atcgggtgag tccgccccgg tcactgccac ggccgaactc
     3001 cgcaccgtga cggtcgtctt cacggtgact gtgcccgaca ccacggatgc ctctgggcgc
     3061 gtcgtcaaca tcgccgggac gctcaatcgg ctcgacggcg gtctgcctga gtggnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gaaaaggacg
     3241 cggcctgcgg cgagatcggc aaccggcagc tgaccctcag ctacggcgct tcaggcacgc
     3301 agaacgtcaa cgacgtggtg ggcaactggc gtaacagcgc gaacttcgga ggcgtctgcg
     3361 ggaactaacg cggagcgtcg tgtttggagc gtggagcgtg gagcgataag cgattctctc
     3421 gctccacgct ctacgctcca tacttcacgg cttcggcgtc cgcgtcggcc gcaggatcgc
     3481 cagggtgccg acatccgccg ggcctctggg ttggatctgc caaccggccg atcgggtcgt
     3541 cgtcgccgat tgcacgatga ccaggccggt cagcgtgatc gtctccccac ggctcacctg
     3601 gggacgtttg agcccgttgg ccgccccgaa cgggatccgc acaaccccgc tgccatcgtc
     3661 gatccacagt gccgacgatt ccaaccgcgt cacccggccg gtcacggtga cgacctcgta
     3721 gggatgctgc gccgcgccgc ctgtcgtgat cgggactggc gccggcactg cgcccggtgg
     3781 cgagaacgac gcctgcggca ggagacgaac atagaactcc agtacgccgc cgcgaaggcg
     3841 actcagaccc agcgcatgaa agccgtttcc aggaatcacg ccgggtggcc aggcgccccg
     3901 gcccagatag accgggatcg agcctgtcgc atcctgaacc acgagcaccc gcgcaccgag
     3961 caccccaggc ggcacaatca ctgtcgcatc aagttcgacc cacgcgccca ccggccaggt
     4021 cttcaggacc gcgatcggca ccggcgtcac agttggtgtc agcgtagcac tgggcttcgg
     4081 cgtccgcgtc ggcttgagag tcttggtggg tttgggcgtg cgcgtcatcg acggcgtctt
     4141 ggtcgggctc ggcgtccgcg tcatcgacgg cgtcttcgtc gggctcggcg tccgcgtcgg
     4201 gctcggcgtc cgcgtcatcg atggcgtctt cgtcgggctg ggcgtgcggg tcatcgacgg
     4261 cgtcttcgtc gggctcggcg tgcgggtcat cgacggcgtc ttggtcgggc tgggcgtccg
     4321 cgtcatcgac ggcgtcttcg tcgggctcgg cgtccgcgtc gggctcggcg tccg
//
LOCUS       DAORNF010000223         4367 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_14985_length_4367_cov_1.726809, whole genome shotgun sequence.
ACCESSION   DAORNF010000223 DAORNF010000000
VERSION     DAORNF010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4367
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14985_length_4367_cov_1.726809"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            154..>666
                     /locus_tag="PLV68_09795"
     CDS             154..>666
                     /locus_tag="PLV68_09795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01582.1"
                     /translation="MADGLASDPDHPLPLPSGLLAGLGNQLDLRRQLSPPAYLAFENT
                     AWVPTQAMLSANAAEQSNKAGLEALASTQIAGSIPVLTGMPGRGPGTGAIEAGVLHLA
                     KPFDADWTLTVDGTTIDPRPAFGTSMAFDVPVAGTARLAYDTGFGRHLAIQVQALAWL
                     ALLLAASRISW"
     assembly_gap    667..779
                     /estimated_length=113
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <780..1038
                     /locus_tag="PLV68_09800"
     CDS             <780..1038
                     /locus_tag="PLV68_09800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01583.1"
                     /translation="TAAPWIVAPGTATAGHGEIDAATGTETDALIGTEIDTEIDAQAE
                     IGADTGADTRADPEADTGDDDDSTVALQRPASDSDETGGTS"
     gene            1053..>1710
                     /locus_tag="PLV68_09805"
     CDS             1053..>1710
                     /locus_tag="PLV68_09805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01584.1"
                     /translation="MIGSFAVVLVALAVAVSRGSVTPATASFGAVPLASRPYASHLAP
                     ITTTWYCPGMATGPDGGGEVVVANPTDVELSGSITVMGTVGVAPATQPLSVPARGRLS
                     VDIAAIVDAAFVAAIVEIDGGEGMVEQRAANPGGDAVSSCTTQTSSSWYFADGYTLSN
                     SIEQIILVNPSADTASVNMRFVTDKGSRRPAALQGRTIAPRSVQVLSIAESGFRDEPI
                     I"
     assembly_gap    1711..1792
                     /estimated_length=82
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1808..>2058
                     /locus_tag="PLV68_09810"
     CDS             1808..>2058
                     /locus_tag="PLV68_09810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01585.1"
                     /translation="MGSPVVSDQVWFAHGQGGASITEQYVIFNPTDQAVSVDVLVLGI
                     GLPETFVPPEPVDIAAGGVATVDTATFTGLPEGAHALVFS"
     assembly_gap    2059..2260
                     /estimated_length=202
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2261..2494
                     /locus_tag="PLV68_09815"
     CDS             <2261..2494
                     /locus_tag="PLV68_09815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01586.1"
                     /translation="PAGAVPVTGLEAVPLPASAIVRIPLTDQTVFDQSLIVTSTQRVL
                     VERRLPRGHDQQGRSASWALPECGPCLFSSRQS"
     gene            2467..2913
                     /locus_tag="PLV68_09820"
     CDS             2467..2913
                     /locus_tag="PLV68_09820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRE01587.1"
                     /translation="MPVLVAAILIVVVGTAALIVQRRRHADPPTQPVYAAPAQLDRAD
                     FDATNEWLVVTFTSASCSTCADVKRKAAVLASAEVGVVDVEYSAAGAVHARYNIDAVP
                     TLVIADRDGVVRGSFLGPVSATDLWAAVARARDPESVPEHGCGSAD"
     gene            complement(2917..>3930)
                     /locus_tag="PLV68_09825"
     CDS             complement(2917..>3930)
                     /locus_tag="PLV68_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsH"
                     /protein_id="HRE01588.1"
                     /translation="NRPDILDPALLRPGRFDRQIVVPLPEAEERLAILKVHSRDKRMG
                     PDVDLEVMAKATPGMSGADLANLVNEAALVAVRRGSKEIGRIDFENARDRVVLGARRE
                     SMVLSVEERRATAYHEGGHAILATVLPHGDPLHKVTILPRGMALGVTWSLPQERHTYS
                     KEYFEDTICKAMGGRVAEMIVFDNLNSGAANDLEQATNIARHMVREWGMSDRIGPMAW
                     GSQQHVFLGEDLMSSGREYSDSTARILDEEISGILHAQQTRARELLTKHRRGLDLLAE
                     ALLEHETIDGAEVARLIQQGLGEVPTAGGSAVSAGTGDAGTSADNGHTDAANAVVAPE
                     AAD"
     assembly_gap    3931..4147
                     /estimated_length=217
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4148..>4367)
                     /locus_tag="PLV68_09830"
     CDS             complement(<4148..>4367)
                     /locus_tag="PLV68_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRE01589.1"
                     /translation="AGVKQEIKEVVDFLKMPERFREIGARVPKGMLLVGPPGTGKTLF
                     ARAVAGEAGVGFLSVTGSDFMEMFVGVGA"
BASE COUNT          535 a         1342 c         1276 g          600 t
ORIGIN      
        1 gaccccaggc ccagacatcg acctcgatcc ggtccttctg cacgtcggct ccggccgtca
       61 ggaacaaccc gcctgccggc acgatgccag gtttccacgc ctcgcgccgg tcgcctgctt
      121 gcgccgtacg cgatccgcta cctggtgatc ccggtggccg acggattggc atcagacccc
      181 gatcatccgc tgccgctgcc gagcgggctg ctggccggtc tcggcaacca actcgaccta
      241 cgccgccaac tctcgccgcc ggcgtacctg gcgttcgaga acacggcgtg ggtgccgacg
      301 caggcgatgc tcagcgccaa cgcggccgag cagagcaaca aggccggtct ggaagcgctc
      361 gcctccaccc agatcgccgg gtcgatccct gtgctcaccg gcatgcccgg tcgcggaccc
      421 ggcacgggtg cgatcgaagc cggcgtgctc cacctcgcca agccgttcga cgccgattgg
      481 acgctcacgg tcgacggcac cacgatcgac ccgcggcccg cattcggtac gtcgatggcc
      541 ttcgacgtac cggtcgcggg cacggcccgg ctggcctatg acaccgggtt cggccgccac
      601 ctggcgatcc aggtccaggc gctggcctgg ctggcgttgc tgttggccgc cagccgtatc
      661 agttggnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc
      781 accgcggccc cgtggatcgt tgcccccgga acggctaccg ccggtcacgg cgagatcgac
      841 gccgcgacag ggaccgagac cgacgcgctg atcggcaccg agatcgacac cgagatcgac
      901 gcccaggcgg agattggggc cgacaccggg gccgataccc gggccgaccc tgaggccgac
      961 accggggacg acgacgactc gaccgtggcg ttgcagcgcc cagccagcga cagcgacgag
     1021 acgggcggta catcgtgaac cttcgccgga ccatgatcgg ttcgttcgca gtcgtgctgg
     1081 ttgcgttggc cgtcgccgtg tcgcgcggca gcgtcacgcc cgccacggcg tcgttcggcg
     1141 cggtgccgct ggccagccgt ccgtacgcca gccacctcgc cccgatcacc accacctggt
     1201 attgcccggg catggccacc ggcccagacg ggggcggtga ggtcgtggtc gccaacccga
     1261 ccgacgtcga actatccgga tcgatcacgg tcatgggcac cgtcggcgtg gctccggcca
     1321 cccagccgct ctccgtccca gctcgcggcc ggctctcggt cgacatcgcc gcgatcgtcg
     1381 atgccgcctt cgtcgccgcg atcgtcgaga tcgacggcgg cgagggaatg gtcgaacagc
     1441 gcgccgccaa cccgggtggc gacgcggtgt cgtcgtgcac cacccagacc tcgtcgagct
     1501 ggtacttcgc cgacggctac acgctgtcga acagcatcga gcagatcatc ctggtcaacc
     1561 cgtcggccga cacggccagc gtcaacatgc gcttcgtcac cgacaagggc tcccgccgcc
     1621 cggctgcctt gcagggccgc accatcgcgc cgcggtcggt gcaggtgctg tcgatcgccg
     1681 agagcggctt tcgcgacgag ccgatcatcg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngggtactc
     1801 gttgacgatg ggttcgccgg tcgtatccga ccaggtgtgg ttcgcccacg gccagggtgg
     1861 tgcatcgatc accgagcagt acgtcatctt caatcccacc gaccaggcgg tgtcggtcga
     1921 cgtgttggtg ctgggcatcg gtctgcccga gacgttcgtg ccgcccgagc cggtcgacat
     1981 cgcagccggc ggggttgcca ccgtcgatac ggcgaccttc accggtttgc ccgagggtgc
     2041 gcatgcgctg gtgttctcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ccggccggtg ccgtgccggt
     2281 caccgggctg gaagcggtgc cgctgcccgc ctcggcgatc gtgcgcatcc cgctcaccga
     2341 ccagaccgtg ttcgaccagt cgctgatcgt cacctcgacc cagcgagtgc tggtcgagcg
     2401 tcgcctcccg cgcggccacg accaacaagg ccgcagcgcg tcgtgggcgc tgccagagtg
     2461 tgggccatgc ctgttctcgt cgcggcaatc ctgatcgtcg tcgtcgggac ggccgcactg
     2521 atcgtgcagc gccgccgcca cgccgacccg cccacccaac cggtctacgc ggcccccgct
     2581 caactcgacc gggccgactt cgatgccacc aacgagtggt tggtggtgac cttcacgtct
     2641 gcgtcgtgct ccacgtgtgc cgacgtgaag cgcaaggccg ccgtgctggc cagtgccgag
     2701 gtcggcgtcg tcgacgtcga atacagcgca gccggcgccg tgcacgcgcg ctacaacatc
     2761 gacgccgtgc cgaccctggt gatcgccgat cgcgacggcg tggtgcgggg cagcttcctc
     2821 ggcccggtca gcgcgaccga tctgtgggcg gccgtcgcgc gggcgcgcga ccccgagtca
     2881 gtgcccgagc acggctgcgg ctcagccgac tgagattcag tcagccgctt cgggagcgac
     2941 gacggcgttg gccgcgtcgg tgtgaccgtt gtcggcgctg gtcccggcgt cgccggtacc
     3001 ggcggacacg gccgagccac cggcggttgg cacctcgccc aggccctgct ggatgaggcg
     3061 ggccacctcg gcgccgtcga tggtctcgtg ctcgagcagc gcttcggcga gcaggtcgag
     3121 gccgcgacga tgcttggtga gcaactcgcg ggcccgggtc tgctgggcgt ggaggatgcc
     3181 cgagatctct tcgtcgagga tgcgcgcggt cgaatcgctg tactcgcggc cgctgctcat
     3241 caggtcttcg ccgaggaaca cgtgctgctg gctgccccat gccatggggc cgatgcggtc
     3301 actcatgccc cactcgcgca ccatgtggcg ggcgatgttg gtggcctgct ccaagtcgtt
     3361 ggccgcgccg ctgttgaggt tgtcgaacac gatcatctcg gccacgcggc cacccattgc
     3421 cttgcagatc gtgtcttcga agtactcctt ggagtacgtg tgacgctctt gcggcaggct
     3481 ccaggtgaca ccgagggcca tgccccgggg caggatggtg accttgtgca gcgggtcgcc
     3541 gtgaggcagc acggtggcga gaatggcgtg accaccttcg tggtaggccg tggcccgacg
     3601 ctcttcgacc gataacacca tgctctcccg gcgggcaccg agcaccaccc ggtcgcgggc
     3661 gttctcgaag tcgatccggc cgatctcctt ggagccgcgg cgcacggcca ccagggctgc
     3721 ctcgttgacc aggttggcca ggtcggcgcc gctcatgcca ggcgtggcct tggccatcac
     3781 ctcgaggtcg acgtcgggac ccatccgctt gtcgcggctg tgcaccttga ggatcgcgag
     3841 gcgctcttcg gcttcgggca gcggcaccac gatctggcgg tcgaagcggc cgggacgcag
     3901 cagcgccggg tcgaggatgt cgggccggtt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4141 nnnnnnngcg ccgacgccga cgaacatctc catgaagtcg gagccggtga ccgacaggaa
     4201 gccgaccccc gcttcgccgg cgacggcgcg ggcgaacagg gtcttgccgg taccgggagg
     4261 gccgaccagc agcatgccct tgggcacccg ggcgccgatc tcgcggaacc gctcgggcat
     4321 cttcaggaag tcgacgacct ctttgatctc ttgcttgacc ccggcgt
//
LOCUS       DAORNF010000224         4367 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_14986_length_4367_cov_1.630798, whole genome shotgun sequence.
ACCESSION   DAORNF010000224 DAORNF010000000
VERSION     DAORNF010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4367
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14986_length_4367_cov_1.630798"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="PLV68_09835"
     CDS             complement(<1..184)
                     /locus_tag="PLV68_09835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01590.1"
                     /translation="MAAASAIILDDRLLIEELLVGLDVEGERHTTTYWYYRACRAAVS
                     GAGGHLSGPFLGLDIAH"
     gene            complement(185..424)
                     /locus_tag="PLV68_09840"
     CDS             complement(185..424)
                     /locus_tag="PLV68_09840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HRE01591.1"
                     /translation="MSGASTAKVSHRGQTNLPAALRHRWGIEHGGEVGIIDLGDAALL
                     VPGGMRTARNELRRVLHDRYEVGLAGIEDPDLVDQ"
     gene            complement(533..604)
                     /locus_tag="PLV68_09845"
     tRNA            complement(533..604)
                     /locus_tag="PLV68_09845"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(570..572),aa:Lys,seq:ttt)
     assembly_gap    799..911
                     /estimated_length=113
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<912..>1465)
                     /locus_tag="PLV68_09850"
     CDS             complement(<912..>1465)
                     /locus_tag="PLV68_09850"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRE01592.1"
                     /translation="AAGLGEAADVPAVTISRVGEGAAASGVESGETESVAADRHTPRL
                     DELVVVDLSSLWAGPLCAHLLGLGGTQVIKVESWQRPDGARRGPKRFYDLLHGGHQSV
                     AFDFDDPDQRRALRELVEHADVVIEASRPRALRALGIDRERIMATTDRGHGPRVWLSI
                     TAHGRSDDQSRIGFGDDAAVGGGL"
     assembly_gap    1466..1532
                     /estimated_length=67
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1533..1840)
                     /locus_tag="PLV68_09855"
     CDS             complement(<1533..1840)
                     /locus_tag="PLV68_09855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01593.1"
                     /translation="MGVPAALVAGVQRGGRDITRLTAAMGEAVELDWLALLGERAAIV
                     GLGRRGDVSCGGAARMLRVSDGWLALSLARADDVAAVPAWLEVDGLPEAAGNAAVGEH
                     "
     gene            2146..3096
                     /locus_tag="PLV68_09860"
     CDS             2146..3096
                     /locus_tag="PLV68_09860"
                     /inference="COORDINATES: protein motif:HMM:NF027439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HRE01594.1"
                     /translation="MDEQIAGGLRAVLVDTRPDPSTTQQLDSLGSLPLVVVGVAHETG
                     AGLAVADVVVGPDDPALAAIITTIETNPIAATSLAVLLRMTTALDTSSGDAVDVALAA
                     ESATYSTLQAGAEFATWRAGRSGSAPAPDPADAVVIERSSADDRQLTIWLNRPHRHNA
                     FSRSMRDALCDALALAVVDDQITSIELRGNGPSFCSGGDLGEFGGFDDPASAHHTRLT
                     RSPARLMHRIGDRCTAYLHGFCIGAGIELPAFCHRIVATPDARIGLPEVSLGLVPGAG
                     GTVSLPRRIGRQRTAYLALSGVQLTAPEALAWGLVDAIAD"
     gene            3268..3933
                     /locus_tag="PLV68_09865"
     CDS             3268..3933
                     /locus_tag="PLV68_09865"
                     /inference="COORDINATES: protein motif:HMM:NF005595.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HRE01595.1"
                     /translation="MSWPTSGRRSTATTTRGAGKGCSSGGSFELLDEMATGGFDTRLR
                     VLREARDLVANILDCNKPIVSAIHGPAVGAGLVAAMLADVSVAARTARIIDGHTRLGV
                     AAGDHAAVCWPLLCGMAKAKYYLLTCDTLSGEEAERIGLVSLCVEDDQVHARALAVAE
                     QLAAMAQPSLSFTKQTLNHWYRQAFPAFDASLAYEFLGFGGPDVLEGIASHREKRPPR
                     FNR"
     gene            complement(3982..>4367)
                     /locus_tag="PLV68_09870"
     CDS             complement(3982..>4367)
                     /locus_tag="PLV68_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRE01596.1"
                     /translation="PEQRVNLDYVVDYIARRSLLAVDGGWRWKFDRNMLASFKGSMRE
                     VALPYLSNVRCRLALLRAQYGLVTPDIGRAMFERMGRVTPVIELPQAGHHPMVDQPLV
                     LITALRALLGDWDHSEPHRPATSPG"
BASE COUNT          683 a         1523 c         1365 g          616 t
ORIGIN      
        1 ggtgggcgat gtcgagaccc aggaacggcc cagagagatg gcctcccgct ccgctcaccg
       61 cggctcgaca cgcccggtag taccagtacg tcgtggtgtg gcgttcaccc tcgacgtcga
      121 ggccgacgag cagctcctcg atcagaagcc ggtcatcgag gatgatcgcc gaggccgctg
      181 ccatctactg gtcgaccagg tctgggtcct cgatacctgc gaggcccacc tcgtagcggt
      241 cgtgcaacac acgacgaagc tcattccttg ccgtccgcat gccaccaggg accaagagcg
      301 ccgcatcacc gagatcgatg atcccgacct cgccaccgtg ttcgatcccc cagcgatgac
      361 gcagcgcagc gggcagattg gtctggccgc gatgagagac cttggcagtt gacgcaccag
      421 acatgacctg cattgtaccg aaggcacacc tccaaggcgc agcgtcgtgg tgcgcaacca
      481 atcagccgaa gccccactga gaggaatcag ccgaagtaac acatctcgat cgtgcgcccc
      541 ctgggtttcg aacccagaac ctgcggatta aaagtccgtt gctctgccgt tgagctagag
      601 gcgcgtcgcg ccagacgata ccaatcagat ctggatgccg aaccgggcca ggaccgcagc
      661 ggtatcggca ccgatgcgag cagccaccgc gtggggggca cgggctcgcg gcgcggcgat
      721 ggcggattcg tcgatcgtga cgcggggtcc ggtggccacc gacgcggcga ccccggcgag
      781 ggtgacgtcg aggcaccann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      901 nnnnnnnnnn nccaagccac caccgacggc cgcgtcgtcg ccgaacccga tccggctctg
      961 gtcgtcgctg cggccgtgag cggtgatcga cagccacact cgcggtccgt gtccgcgatc
     1021 ggttgtcgcc atgatccgct cgcggtcgat gccgagcgcg cgcaacgccc gcggtcgcga
     1081 tgcttcgatc accacgtcgg cgtgttcgac caactcgcgc agtgctcgcc gctggtcggg
     1141 gtcgtcgaag tcgaacgcga cggactgatg gccgccgtgg agcaggtcgt agaaccgctt
     1201 tgggccgcgg cgcgcaccgt cgggacgctg ccagctctcg accttgatca cctgcgtccc
     1261 acccaagccg agcagatgcg cgcacagtgg tcccgcccac aacgacgaca ggtcgaccac
     1321 aaccagttcg tcgagccgcg gggtatgacg atcggccgct acagactcgg tctcgccgga
     1381 ctcgaccccc gatgccgccg cgccctcgcc gacacgactg atcgtcacgg cgggcacatc
     1441 ggccgcctcg ccgagtccgg ccgcgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnatgtgctc gccgacggca gcgttcccgg
     1561 cggcttcggg cagcccatcg acctccaacc atgccggtac ggcagcgaca tcgtcggccc
     1621 gcgccagcga gagggccaac cagccgtcgc tcacccgcag catgcgcgcc gcgcccccgc
     1681 aactcacgtc gccgcgacgg cccaacccga cgatcgcggc tcgctcgccc agcaacgcca
     1741 accaatcgag ctcgaccgct tcccccatcg ccgctgtcaa tcgcgtgatg tcacgtccgc
     1801 cgcgctgcac accggcgacc agcgcggcgg gcacacccaa cggcgcccga ccaacccgcc
     1861 ccgacagtcc cgcggctccc gacgagtgcc atgcagctgc ggactcgtcg gcgccaacca
     1921 ccgagtcgct gtgatcggcg gcccctgtct caccacgccg cccgtctcct tccctcgtgc
     1981 actcgaagtg ccaacgatac ggcgacgccg gtccccagca cggcgccacg agcaggactc
     2041 cccacgaccg gcggcggccg cgcaatgccg tccgggtact gtcgagagat cgagcactcg
     2101 ttgatctccc aggccgaact ggcggaactg gttcggtcgc cgtacgtgga cgaacagatc
     2161 gcaggtgggc tgcgggcggt gctggtcgac acccggcctg atccgtcgac gacccagcaa
     2221 ctcgattcgc tcgggtcgct gcccctcgtg gtggtcggtg tggcacatga aacaggggcg
     2281 gggctcgccg tcgccgatgt ggtcgtcggg ccggacgacc cagcgctggc agcgatcatc
     2341 acgacgatcg agaccaaccc gatcgcggcc acgtcgctgg cggtgctgct gcggatgacc
     2401 accgcgctcg acacatcgtc gggtgatgcg gtcgacgtcg ccttggcagc ggagtcggcc
     2461 acctactcca cgctgcaggc gggtgccgag ttcgcgacat ggcgagcagg acgaagcggg
     2521 tcggctccgg cacccgatcc tgccgacgct gtggtgatcg agcgctccag cgcggacgat
     2581 cggcagttga cgatctggct caatcggccg catcggcaca acgcgttcag ccgctcgatg
     2641 cgcgatgccc tgtgcgacgc gttggctctg gcggtcgtcg acgaccagat cacatcgatc
     2701 gagctccgtg gcaacggtcc gtcgttctgc agcgggggcg acctcggtga gttcggcggc
     2761 ttcgacgacc cggccagcgc gcaccacacc cggctcacgc ggtcgcccgc gcggctgatg
     2821 catcgcatcg gcgaccgctg caccgcatac ctgcacgggt tctgcatcgg ggccggcatc
     2881 gaactgcccg cgttctgcca ccgcatcgtc gccacgccag acgcgcggat cgggctgccc
     2941 gaagtcagtc tcggcctggt ccccggtgcg ggaggaaccg tgagcctgcc acgacgcatc
     3001 ggccgccagc gcaccgccta tctcgcgctc agcggtgtcc agctcaccgc acccgaagcc
     3061 ctcgcctggg gccttgtcga cgccatcgca gattgacgcc gtcgcagact gacgccatcg
     3121 cgggcgcagg gccgcgggta cggtcccagg ccatggccga cttcagcgct ctgtatccca
     3181 gtctccggtt cgaccgtccc tccgacgggg tgttgcggat caccctcgac gcgcccgggc
     3241 tcaactcggt gtcgcccgcc gcccaccgtg agctggccga cgtctggcag acgatcgacc
     3301 gcgacgacga cacgcggcgc aggcaaaggc tgctcctcgg gtggatcgtt cgagctgctc
     3361 gacgagatgg caacgggcgg cttcgacacc cgcctgcgcg tgctgcgcga ggcccgcgat
     3421 ctggtggcca acatcctcga ctgcaacaag ccgatcgtgt cggccatcca cggcccggcg
     3481 gtgggcgccg ggctggtcgc ggcgatgctg gccgacgtgt cggtcgcggc gcgcacggcg
     3541 cggatcatcg acggtcacac ccgcctcgga gtcgccgcgg gcgaccatgc cgccgtgtgc
     3601 tggccgttgc tgtgcgggat ggccaaggcc aagtactacc tgctcacctg cgacacgctg
     3661 agcggcgagg aagccgagcg catcgggttg gtttcgctgt gtgtcgagga cgaccaggtc
     3721 catgcccggg cgttggccgt ggccgaacag ctggcggcga tggcccagcc ctcgctgtcg
     3781 ttcaccaagc agacgctgaa ccactggtac cgccaggcgt tccccgcctt cgacgcgtcg
     3841 ctcgcctacg agttcctcgg cttcggtggc cccgacgtgt tggagggcat cgcgtcccac
     3901 cgcgagaagc ggccgcctcg cttcaaccgg taggacccat ctcaaccagc ggccctcacg
     3961 tcaaccggca ggggcctcac gtcaaccagg ggacgtcgcc ggacgatggg gctcggagtg
     4021 gtcccagtcg ccgagcaacg cccggagcgc cgtgatcagc accaacggct ggtcgaccat
     4081 cgggtggtga cccgcctgcg gcagctcgat cacgggggtc acgcggccca tcctctcgaa
     4141 catcgcgcgg ccgatgtcgg gcgtcaccaa cccgtactga gcgcgcagca acgccaaccg
     4201 gcagcgcaca ttggagagat atggcagggc gacctcgcgc atgctgccct tgaacgacgc
     4261 cagcatgttg cggtcgaact tccaccgcca gccaccgtcg acggccagca gcgagcgacg
     4321 ggcgatgtag tcgaccacgt agtcgaggtt cacccgctgt tcgggaa
//
LOCUS       DAORNF010000225         4326 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_15149_length_4326_cov_1.504566, whole genome shotgun sequence.
ACCESSION   DAORNF010000225 DAORNF010000000
VERSION     DAORNF010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4326
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15149_length_4326_cov_1.504566"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1776
                     /locus_tag="PLV68_09875"
     CDS             <1..>1776
                     /locus_tag="PLV68_09875"
                     /inference="COORDINATES: protein motif:HMM:NF020083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRE01597.1"
                     /translation="ALPQTSLFAARFRECAARALLLPRRRPDRRTPLWQQRQRSADLL
                     AVAAKYPSFPILLETSRECLQDVFDVPALREVLSQLRSRAIRVVSVETPKASPFAQSL
                     LFSWIATYMYEGDAPLAERRAAALALDRDLLRDLLGAEELRELLDADVLADLELEIQC
                     LTDTRRARDVDEMHDVLRRVGDLTADEADLRAHGAAGLWLATLVAQRRAIEVAIGDET
                     RFVAAEDAAKYRDALGCAIPVGLPLAFTEPVVRPLEELLRRYARTHGPFVTTGPARRF
                     AVSPERIAGALTALEAEGRLVVGEFRPEGLAREWCDADVLRQLRRRSLASLRREVEPV
                     EQDALARFVAQWHGIPPERQGADALVEALGVLQGAALVASALETDILPQRIRGYRAVD
                     LDEMCTNGDVVWIGAGSIGANDGRVRLYFADQLPVLVNGLEAATKPQGAVHDAIRTLL
                     AERGASFWNQIRAAAPAATDTEVLTALWDLVWAGEITNDSLGPLRALLAGGVRKPTVP
                     RQRAGRRPARLNRIGPPAAAGRWSLVAPLLRPVPTSTAVAHASALQLLERHGVLTREA
                     VAAEGIVGGFSAVYGVLKVLEERGQV"
     assembly_gap    1777..1864
                     /estimated_length=88
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1865..2256
                     /locus_tag="PLV68_09880"
     CDS             <1865..2256
                     /locus_tag="PLV68_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01598.1"
                     /translation="GTLLLVGQGGLVLASTDPAQPYGATLSWPDRGERAGRAARAAGS
                     SVVLAAGTPLAWFDRRSSHLVLFPAGHDDTRWAQALAGMVGEGIRRSIEVRKIDGEPI
                     PATSPAADALRAAGFVDGYRGLTYRRS"
     gene            complement(2378..>2773)
                     /locus_tag="PLV68_09885"
     CDS             complement(2378..>2773)
                     /locus_tag="PLV68_09885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01599.1"
                     /translation="GYLSDGAIDDVPDERLLDTRTSAKPANWSQTVVTGRPNRSAIIS
                     LVATQTSLATYLQVLPCGAEPGATSNLNADRSGQTIAGLAVVRFDANGEVCVFVQHAT
                     HVVVDLQAYLSDAAFEDITDLRILDTRTR"
     assembly_gap    2774..2844
                     /estimated_length=71
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2845..>4326)
                     /locus_tag="PLV68_09890"
     CDS             complement(<2845..>4326)
                     /locus_tag="PLV68_09890"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HRE01600.1"
                     /translation="DNASFQAKYQRETDRWIEGDVELRDLIRQLNTLTGDAKREWIYA
                     NVDVASVVNLLATVATIQHYDIGHKNFRLIYDEHGMWSALPTDFDLSFGRGWREPGTI
                     VNDIVIVNDNFDAPRGPLFGIFWDDPELSAMVARRARTLAEEMLNPLLVAERVADLGA
                     QIAIDAALDRAVWGTYDTPQTPADATVVMMSDFVKPQYQRMLGVLTETGRVAATTQPD
                     KPAVSVTVDDVSGVVALTNDSADSVDLSLFRIDSIGLVVPGGTVVLPGRSAYFVPTSW
                     GSVSGSFGHASVGYYSGSLTTATDFDLIGPGVTSAQYVSPPTYSPLPGDGRVPPGSHV
                     AISGRPNRSAVVSIVATETAAAGYLQVLACDATPGTTSNLNTDRANQTIAGLAVIRFD
                     ANGDACVFNQSATHIVVDLQGYLDDGAIDDVPDQRLIDTRSGARPAAGAQVRIHGEAN
                     RSAIVSLIATENRSASYLQVLACGAVPGATSNLNTDRANQIIAG"
BASE COUNT          594 a         1401 c         1498 g          674 t
ORIGIN      
        1 ggccctgccg cagacctcgc tcttcgcagc ccgcttccgc gagtgcgcgg cccgtgccct
       61 gctgttgccg cggcggcgcc ccgatcggcg cacgccactg tggcagcaac ggcagcgttc
      121 ggctgatctg ctcgcggtgg cggccaagta ccccagcttc ccgatcctgc tcgagaccag
      181 ccgcgagtgc ctgcaggacg tgttcgacgt gccggcgttg cgagaggtgt tgagccagtt
      241 acgcagccgg gcgatccgcg tggtcagtgt cgagaccccc aaggcatcgc cgttcgcgca
      301 gagcctgctc ttcagctgga tcgccaccta catgtacgaa ggcgacgcgc ccctggccga
      361 gcgacgcgcg gcggcgttgg cgctcgaccg cgatctgttg cgcgatctgc tcggtgccga
      421 agaactgcgc gagttgctcg acgccgacgt gctcgccgac ctcgagctcg agatccagtg
      481 cctgaccgac acccgccgcg cccgcgacgt cgacgagatg cacgacgtgc tgcgccgtgt
      541 cggcgacctg actgccgacg aggccgacct ccgtgcccac ggtgccgccg ggctgtggtt
      601 ggccacgctg gtggcccagc ggcgggcgat cgaggtcgcg atcggtgacg agacccgctt
      661 cgtcgccgcc gaagacgcgg ccaagtatcg cgacgcgctc ggctgcgcga tcccggtcgg
      721 gttgccgctg gccttcaccg agccggtggt ccgtcccctc gaagagctgc tccgccgcta
      781 cgcccgtacc cacggcccgt tcgtcaccac tggcccggcg cggcgcttcg ccgtgtcgcc
      841 cgaacggatc gccggcgccc tcaccgcgtt ggaagccgaa ggccgcctgg tggtcggtga
      901 gttccgtccc gagggcctgg cgcgggaatg gtgcgatgcc gacgtcttgc gtcaactccg
      961 tcgccggtcg ctggcgtcct tgcgccgcga ggtcgaaccg gtcgagcagg acgcgctggc
     1021 ccgcttcgtc gcccagtggc atggcattcc gcccgaacgg cagggcgccg acgcgctggt
     1081 cgaagcgttg ggcgtgttgc agggcgcggc gctggtggcc tcggcgttgg agaccgacat
     1141 cctgccccag cgcattcgcg gctaccgggc cgtcgacctc gacgagatgt gcaccaacgg
     1201 cgacgtggtg tggatcggcg ccggttcgat cggcgccaac gacggccggg tgcggttgta
     1261 cttcgccgat caactgccag tgttggtcaa cgggctggaa gcggcgacca agccgcaggg
     1321 cgcggtgcac gacgcgatcc gcaccctgct cgccgagcgc ggggccagct tctggaacca
     1381 gatccgggcc gcggcaccgg cagccaccga caccgaggtc ttgaccgcgc tgtgggacct
     1441 ggtgtgggcc ggtgagatca ccaacgactc gctcggtccg ttgcgggcgc tgctggccgg
     1501 tggtgtccgc aaaccgaccg tgccgcgaca gcgtgccggg cggcgaccgg cacggctcaa
     1561 ccgcatcggc ccacccgcgg cggccggacg atggagcctg gtggcgccgt tgctgcgtcc
     1621 ggtgccgacg tcgaccgcgg tcgcccacgc cagcgcgctg caactgctcg aacgccacgg
     1681 cgtgctcacc cgcgaggccg tcgcggccga aggcatcgtc ggcgggttca gcgcggtgta
     1741 cggggtgctg aaggtgctcg aagagcgggg tcaggtnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnatggca ccctgctgct ggtcggccag ggcggtctgg tgctggccag caccgaccct
     1921 gcccaaccgt acggtgcgac gttgagttgg cccgatcgcg gcgagcgtgc tggtcgtgcc
     1981 gcgcgcgctg cggggtcgtc ggtggtgctg gccgcgggca cgccgctggc ctggttcgat
     2041 cggcgtagct cccatctcgt gctgttcccg gcggggcacg acgacacccg ctgggcccaa
     2101 gcgctcgccg gcatggtcgg cgaagggata cgccgttcga tcgaggtgcg caagatcgat
     2161 ggagaaccca tccccgccac cagcccggcc gccgacgccc tccgcgcggc aggcttcgtc
     2221 gacggctacc gcggtctcac gtaccgccgc tcatagaccg cgctcgatcg ttcaagctcg
     2281 atcgttcgcg ctcgattgtt cgcgctcgat tggcagtccg tgatcggctg gcgctgatca
     2341 gccacccttc gctacccgag gtccgcggga aacccgctca gcgcgtccgg gtgtcgagga
     2401 tgcggaggtc ggtgatgtcc tcgaaggccg cgtcgctgag gtaggcctgc aggtcgacca
     2461 cgacgtgtgt cgcgtgttgg acgaagacgc acacctcgcc gttggcgtcg aagcgcacca
     2521 ccgccagccc ggcgatcgtc tgaccgctgc ggtcggcgtt gaggttcgac gtcgcccccg
     2581 gctcggcacc gcacgggagc acttgcaggt acgtggccag cgacgtctgc gtcgccacga
     2641 gcgagatgat cgccgaccgg ttgggccgtc cggtgacgac ggtttgcgac cagttggccg
     2701 gcttggcgct ggtccgagtg tcgagcaggc gctcgtcggg gacgtcgtcg atcgcaccat
     2761 cggagaggta gccnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnncccggc gatgatctgg ttggcgcggt cggtgttgag
     2881 gttgctcgtc gcaccaggta ccgcgccaca ggcaagtacc tgcaggtagc tggccgaccg
     2941 gttctcggtg gcgatcagcg acacgatcgc cgagcggttc gcttcgccgt ggatacgcac
     3001 ctgcgcgccc gctgccgggc gcgcgccgga gcgggtgtcg atcaaccgct ggtcggggac
     3061 atcgtcgatc gcgccatcgt cgaggtagcc ctgcagatcg accacgatgt gggtcgccga
     3121 ctggttgaac acgcacgcgt cgccgttggc gtcgaagcgg atcacggcga gtccggcgat
     3181 ggtctggttg gcgcggtcgg tattgaggtt gctggtggtg cccggcgtgg catcgcaggc
     3241 gagcacctgg aggtagccgg ccgcagcggt ctcggtcgcc acgatcgaca ccaccgcgga
     3301 gcggttgggc cgaccggaga tggccacgtg tgaaccgggt gggacgcgcc cgtcacctgg
     3361 caggggggag tacgtgggcg gtgacacgta ctgcgccgac gtgacccccg gcccgatcag
     3421 atcgaaatcg gtggcagtgg tcagcgaacc gctgtagtag ccgaccgagg cgtgaccgaa
     3481 cgagccactc accgaacccc acgaggtggg gacgaagtac gccgagcgtc cagggagcac
     3541 caccgttcca ccgggaacga ccaggccgat gctgtcgatg cggaacaggc tgaggtcgac
     3601 cgagtcggcg ctgtcgttgg tgagtgcgac gacacccgag acgtcgtcga cggtgaccga
     3661 gacggcgggc ttgtcgggtt gggtcgtcgc cgcgacacgg ccggtctcgg tcagcacgcc
     3721 gagcatccgt tggtattgcg gcttgacgaa gtcgctcatc atcaccacgg tcgcatcggc
     3781 tggggtttgg ggcgtgtcat acgtgcccca caccgcgcga tcgagtgcgg cgtcgattgc
     3841 gatctgcgca ccgaggtcgg cgacacgctc ggcgaccaac agcgggttga gcatctcctc
     3901 ggccagcgtg cgggcgcggc gagcgaccat cgcgctgagc tcgggatcgt cccagaagat
     3961 gccgaacagc ggaccgcgcg gcgcgtcgaa gttgtcgttg acgatcacga tgtcgttgac
     4021 gatcgtgccc ggttcgcgcc agccccgccc gaacgacagg tcgaagtcgg tcggcagcgc
     4081 cgaccacatc ccgtgctcgt cgtagatcag ccggaagttc ttgtggccga tgtcgtagtg
     4141 ctggatggtg gcgacggtgg ccagcagatt gaccaccgaa gcgacatcga cgttggcgta
     4201 gatccactca cgcttggcat cgcccgtcaa cgtgttgagc tgacggatga ggtcgcgcag
     4261 ctcgacgtca ccctcgatcc agcggtcggt ctcgcgctga tacttcgcct ggaagctggc
     4321 gttgtc
//
LOCUS       DAORNF010000226         4317 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_15181_length_4317_cov_1.336462, whole genome shotgun sequence.
ACCESSION   DAORNF010000226 DAORNF010000000
VERSION     DAORNF010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4317
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15181_length_4317_cov_1.336462"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    218..399
                     /estimated_length=182
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(570..1118)
                     /locus_tag="PLV68_09895"
     CDS             complement(570..1118)
                     /locus_tag="PLV68_09895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01601.1"
                     /translation="MSRVDRILDLLDNATQTSGEHGYPTGVPDACWRCHGAPGDGPSG
                     VCDECRTVLLVELPPEDGPGAAGCGVFGGRVMFMVIDEVARADWLSANPVIPRRVVLG
                     VDPAAYIDIDEAREALAILERQALDRQALELAGAIASTLVEPSVHARGRTGQRRREVA
                     QRPCRHGNPRNTCRDCSTGRRR"
     gene            complement(1115..>1381)
                     /locus_tag="PLV68_09900"
     CDS             complement(1115..>1381)
                     /locus_tag="PLV68_09900"
                     /inference="COORDINATES: protein motif:HMM:NF019146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5131 family protein"
                     /protein_id="HRE01602.1"
                     /translation="IVGGESGPGARPMHPDWVRDIRDRCQAAGVPFLFKQWGAWVAED
                     QSPSGTAFTSAFDDESGTWRLGKARTGRLLDGRTWDEFPPEAQR"
     assembly_gap    1382..1391
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1392..1941)
                     /locus_tag="PLV68_09905"
     CDS             complement(<1392..1941)
                     /locus_tag="PLV68_09905"
                     /inference="COORDINATES: protein motif:HMM:NF019146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5131 family protein"
                     /protein_id="HRE01603.1"
                     /translation="MSTQIEWTDETWNPVVGCSELSPGCDHCYAVGVAHREMQPAHVG
                     LTIGRKWTGEVRCLPDRLDTPLRWRKPRKVFVNSMSDLFHPSVPAEFIRSLFETMAAC
                     PQHTFQVLTKRPQRLPQMFRNLDGRTEPLPTLPNVWIGTSIETDRYAFRANALRATSA
                     VVRFLSLEPLLGPLPSLDLTGID"
     gene            2034..>2262
                     /locus_tag="PLV68_09910"
     CDS             2034..>2262
                     /locus_tag="PLV68_09910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01604.1"
                     /translation="MSSLVPGLMQYRLEKRKWEAEEQRLDRQELADTARSERIEAAEV
                     AASERALRDADLDRLAALHREWHELHVKVVEV"
     assembly_gap    2263..2485
                     /estimated_length=223
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2486..2813)
                     /locus_tag="PLV68_09915"
     CDS             complement(<2486..2813)
                     /locus_tag="PLV68_09915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01605.1"
                     /translation="MQLIEQIVQIYMNYPDLNTQVLAASIRMEATRPAYLADLGDVVL
                     ACDTMTLWRREPDGLCAVWRGRRDFSSERPYGWFAHGRHTWLLDDVHPLATPVPFKGG
                     QGWSRSI"
     assembly_gap    2834..2843
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2844..3080)
                     /locus_tag="PLV68_09920"
     CDS             complement(<2844..3080)
                     /locus_tag="PLV68_09920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01606.1"
                     /translation="MKVLTLHQPFATLVGLGQKLIETRGQAAPRSVIGQRIAIHAAAK
                     VPTVGFYPHGRILYARTAGPGYGDSERQRAWQAHG"
     gene            complement(3077..3679)
                     /locus_tag="PLV68_09925"
     CDS             complement(3077..3679)
                     /locus_tag="PLV68_09925"
                     /inference="COORDINATES: protein motif:HMM:NF018627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01607.1"
                     /translation="MSGRHVRLLLDMDGPLADFDGHGWARCTELGCEFDVDGPEAQTA
                     RYFTDHMPNRAHRAAARAMIEAPGWFDQLSVTPGAVEGVHRLLDAGFDIWVVTKPLEE
                     NPTCRDDKGRWLRRYLPMLERKLILAPDKSLIVGDVLLDDAPKLAWFERALWRPVIFT
                     APFNGPGSAWDGLPRWRWGDPVDVLTDQALRGCWRSWVTA"
     gene            complement(3676..>3935)
                     /locus_tag="PLV68_09930"
     CDS             complement(3676..>3935)
                     /locus_tag="PLV68_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01608.1"
                     /translation="MGGGGHRHAAGFREVTHMPAGAPAGWTHWWIVGFPGDTTLRFSD
                     LGHALTVADRLMDEDDAGIERPRVSAEEFNAAMHEATGGES"
     assembly_gap    3936..3957
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3958..>4317)
                     /locus_tag="PLV68_09935"
     CDS             complement(<3958..>4317)
                     /locus_tag="PLV68_09935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01609.1"
                     /translation="DRDLWRFDLTGTADVFAAVTSRPYTVEAWDEMMWLPIDRLLVEG
                     RAINRWRDQLIEGCVANAYEHTLFGHRVWVAAAPYAIGSDVAGILAKRNPTLFGAYYV
                     DDGHGRKWGLRSGADGLD"
BASE COUNT          624 a         1330 c         1303 g          613 t
ORIGIN      
        1 gtccgcggtg gtcctgacct gcggagatgc cggattcttg accgtgtccg cggtggcccg
       61 cggcaggagc atcacgttca tgcctgaccg ccccctcggg cgactgcgtc ggccacgctg
      121 tcgaggaaca cgttgatcgc agcgtcaggg ccagcgttca cccaggggcg atccggatcg
      181 ggagtcgcgg tgacgtcgat gcacagcgcg ctcgggtnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna gtaggtaggt ttggtcatcg
      421 gtgtttgctc ctgttctggc gtgccaggcg gcgcgcgtaa cgtcctggcc ggtcatcggc
      481 cttctcgcgc cgcctctggg tgcggcactc gccgcagcgg ctccggcggg tgggccgccc
      541 acagtcgggg cacggccggt gatggccgct catcggcgcc gtcctgtcga gcagtcgcgg
      601 caggtgttcc gcgggttgcc atgccgacac ggccgctgcg cgacctcgcg acgtcgctgg
      661 ccggtccgac ctcgggcgtg tacggacggc tcgacgagcg tcgatgcgat cgcgccggcc
      721 agttcgagcg cctgtcggtc gagggcctgc cgctcgagga tcgcgagcgc ctcgcgggcc
      781 tcgtcgatgt cgatgtaggc ggcaggatcg acgccgagca cgacgcgccg ggggatcacg
      841 ggattcgcag acagccagtc agccctggcc acctcgtcga tcaccatgaa catcacccgc
      901 ccgccgaaga cgccgcaccc tgcagcacct gggccgtcct ccggtgggag ttcgaccagc
      961 agcaccgtgc ggcattcgtc gcacacaccg gatggtccgt caccgggagc gccatggcac
     1021 cgccaacacg cgtccgggac accggtcggg taaccgtgct cgccgctggt ctgtgtcgcg
     1081 ttgtcgagca ggtcgaggat gcggtcaacg cggctcatcg ctgcgcctcc ggtgggaact
     1141 cgtcccacgt ccgaccatcc agcaaccgac cggtccgggc tttgccgagc cgccacgtgc
     1201 cggactcatc atcgaacgcc gacgtgaacg ctgttcccga gggggactga tcctcggcga
     1261 cccacgctcc ccactgcttg aacaggaacg gcacacctgc cgcttggcag cggtcacgga
     1321 tatcgcgcac ccaatccggg tgcatcggcc tggccccggg gccggactcg ccgcccacga
     1381 tnnnnnnnnn naatcgatgc cggtgaggtc gagcgacggg agcggaccga gcaacggctc
     1441 aagcgacaag aaccggacca ccgccgaggt cgcacgcagc gcgttcgcgc ggaacgcata
     1501 tcggtcggtc tcgatagacg tgccaatcca cacgttcggg agggtcggca acggctcagt
     1561 gcggccgtcg aggttgcgga acatctgcgg cagccgctgc ggtcgcttgg tgagcacctg
     1621 gaacgtgtgt tgcgggcagg cggccatcgt ctcgaacaag ctgcggatga actcggcggg
     1681 cacgctcgga tgaaacaggt ccgacatgct gttgacgaac accttgcgcg gcttgcgcca
     1741 ccgtagcggg gtgtccaacc gatccggcag acagcgcacc tcacccgtcc acttccggcc
     1801 aatcgtcagg ccaacgtggg cgggttgcat ctcgcggtga gcgacaccga ccgcgtagca
     1861 gtgatcgcag ccaggcgaga gctcgctgca gccgacgacg gggttccacg tctcgtcggt
     1921 ccattcgatc tgagtactca cgacgaccac caatccccgt cgcttctacg ggctaccttg
     1981 cgtgcatggc cggagagtgg gcgatcgtcg cagcagcagt cggaggtgct ggcgtgtcca
     2041 gcttggtgcc tggtctgatg cagtatcggc ttgaaaagag gaagtgggaa gctgaggagc
     2101 agcgcctcga ccggcaggag ttggccgata ctgcacggtc ggaacgcatc gaggccgccg
     2161 aggtcgcggc gtcggagagg gcattgcgtg acgctgacct tgatcgactc gcggcgctgc
     2221 accgggagtg gcacgagttg cacgtcaaag tcgtcgaagt tgnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnncgatc gaccgtgacc atccctgccc gcccttgaac
     2521 ggaaccggcg tggccaacgg gtgcacatcg tcaagcaacc acgtgtgacg gccatgcgcg
     2581 aaccagccgt acggccgctc cgatgagaag tcacgtcggc cgcgccacac agcgcacagc
     2641 ccgtcaggtt cgcgacgcca gagcgtcatc gtgtcgcacg ccagcacgac gtcgccgagg
     2701 tcggcgaggt aggcggggcg tgtggcttcc atgcggatgc tggcggccag cacctgggtg
     2761 ttcaggtcgg ggtagttcat gtagatctgc acgatctgct cgatcagctg caccccgtcg
     2821 atgctgaggt cgtnnnnnnn nnncccgtgc gcctgccatg cgcgctgccg ctccgagtcg
     2881 ccatagcccg ggccggccgt gcgggcgtac aggattcggc cgtgcgggta gaacccgacg
     2941 gtcgggacct tggccgcggc gtggatggcg atgcgttgcc cgatcaccga cctcggtgcg
     3001 gcctgcccac gcgtctcaat caacttttgg ccgaggccta ccaacgtcgc gaagggttga
     3061 tggagcgtca acaccttcac gccgtcaccc acgatcgcca gcagccgcgc agcgcctggt
     3121 ccgtgagcac gtcgacaggg tcaccccacc tccaccgcgg caacccatcc cacgccgacc
     3181 cgggaccgtt gaacggcgcg gtgaagatca ccggccgcca caacgcccgc tcgaaccacg
     3241 ccagcttggg cgcgtcgtcg aggaggacgt cacccacgat caacgacttg tcgggggcga
     3301 ggatcagctt ccgttcgagc atcggcagat agcggcgcag ccatcggccc ttgtcgtccc
     3361 gacaggtcgg gttctcctcc aacggcttcg tgacaaccca gatgtcgaag cctgcgtcga
     3421 ggagccggtg aaccccttcg acagcgcccg gggttaccga cagctgatcg aaccagccgg
     3481 gcgcttcgat catggcgcgg gcggcggcac ggtgcgcgcg gttcggcatg tggtcggtga
     3541 agtacctggc ggtctgagcc tcaggtccgt cgacgtcgaa ctcgcagccg agctcggtgc
     3601 agcgcgccca cccgtgaccg tcaaagtcgg ccaacgggcc gtccatgtcg agcaggagac
     3661 ggacatgacg gccgctcacg actcgccgcc cgtcgcctcg tgcatcgctg cgttgaactc
     3721 ttcggcggac accctcggtc gctcgatccc ggcgtcgtcc tcgtccatca accggtcggc
     3781 gaccgtcaac gcgtggccca ggtcggagaa ccggagggtc gtgtcgcctg ggaaccccac
     3841 gatccaccag tgcgtccacc cggctggggc gccggcgggc atgtgtgtga cctcccggaa
     3901 ccctgccgcg tgacgatgcc cgccaccgcc catctnnnnn nnnnnnnnnn nnnnnnnatc
     3961 aaggccgtca gccccggagc ggagtcccca cttgcgtccg tgcccgtcgt cgacgtagta
     4021 ggcgccgaac agggtcgggt tccgcttcgc gaggatgccg gcgacgtcgc tcccgatcgc
     4081 gtacggcgcc gcggcgaccc acacccgatg cccgaacagg gtgtgctcat acgcgttcgc
     4141 aacgcacccc tcgatcagtt gatcgcgcca ccggttgatc gcccgcccct cgacgagcag
     4201 ccgatcgatc gggagccaca tcatctcgtc ccacgcctcg acggtgtacg gccgtgatgt
     4261 gaccgcggcg aacacgtcgg cggtaccggt gaggtcgaag cgccacaggt cgcggtc
//
LOCUS       DAORNF010000227         4302 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_15253_length_4302_cov_1.432070, whole genome shotgun sequence.
ACCESSION   DAORNF010000227 DAORNF010000000
VERSION     DAORNF010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4302
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15253_length_4302_cov_1.432070"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    375..384
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <385..825
                     /locus_tag="PLV68_09940"
     CDS             <385..825
                     /locus_tag="PLV68_09940"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HRE01610.1"
                     /translation="LEGPTLLAQLLAGADEQALRDAGALLAELHDRLHRIPTMGGGPG
                     TIVHLDLHPDNVMMTPDGPMVIDWANGADGPAELDVAMTWVVMEPVVQVFPEARPLLD
                     GFLDAAGRDLAHRGLRAACDRRLADPNATESERSAIATLRATAT"
     assembly_gap    896..924
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<925..1498)
                     /locus_tag="PLV68_09945"
     CDS             complement(<925..1498)
                     /locus_tag="PLV68_09945"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: protein motif:HMM:NF015319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HRE01611.1"
                     /translation="MSELSEPTRCGWATHHAEEAHYHDTVWGVPQYDPVTLFEYLTLE
                     GAQAGLSWHTILLRKDGYRDAFDQWDIARIAAYTEADVAARMLDTRIIRNRGKITSTV
                     TNARIWQSLADPADYIWSNVGGVPIQTGAITMADVPGFTPVSDAMSKRMKKDGFRFVG
                     TTICYAYMQACGLVNDHVADCWRHAPCTALA"
     gene            complement(1495..1863)
                     /locus_tag="PLV68_09950"
     CDS             complement(1495..1863)
                     /locus_tag="PLV68_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HRE01612.1"
                     /translation="MSNASAPKPVGPYTPVVRAGDWIIVSGQLGVTDGTLVEGGVAAQ
                     TTAAVANIAAQLATVGAALSDVVKTMCFLTDVDTFASFNEAYVAGFGDHRPARSTVGV
                     AGLPLAGASVEIEAWAYRPQ"
     assembly_gap    1952..2056
                     /estimated_length=105
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2057..>2326
                     /locus_tag="PLV68_09955"
     CDS             <2057..>2326
                     /locus_tag="PLV68_09955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01613.1"
                     /translation="ARFPRDLLVPGRQRLVYSLVRDGELQADGPDTLTGKIVRNDDNA
                     VVVASISASKRRIDANLNYWDFHTELSDTGIYTLYVDGASADGTS"
     assembly_gap    2327..2387
                     /estimated_length=61
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2388..2772
                     /locus_tag="PLV68_09960"
     CDS             <2388..2772
                     /locus_tag="PLV68_09960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01614.1"
                     /translation="PRGVTPICTRSPEPCPFHDQTVTEALALGKPVAYMIGTPAHCQF
                     GTCAPGMEFLIAASQRLGDSLAVVHAEVYTDDSATVAAPAMNEYSLVFEPVIYLADAT
                     GTIVTRLDGIWNQGELDEALDALVA"
     gene            complement(2865..3188)
                     /gene="erpA"
                     /locus_tag="PLV68_09965"
     CDS             complement(2865..3188)
                     /gene="erpA"
                     /locus_tag="PLV68_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HRE01615.1"
                     /translation="MITLTDNAASKVKELLEAEGATDLALRVAVRPGGCSGFSYEMYF
                     DGDIAADDEQATFGEGVRVLVDAQSAQLLEGASLDYKDGLNQAGFNITNPNATRSCGC
                     GQSFS"
     gene            3325..4170
                     /locus_tag="PLV68_09970"
     CDS             3325..4170
                     /locus_tag="PLV68_09970"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HRE01616.1"
                     /translation="MASQQPSVAPSPDEIVQLLHAVIDPELGADIVTLGMVPDVAVSA
                     PGADGLVDVTVAVKLTIGGCPLRAQIKKDVESRIALHPAVRDVHIEWGEMTPDERSEV
                     MTKARWNAREHAPDTEVPASTRILAIASGKGGVGKSSVTVNLAAALAAQGFTVGVLDA
                     DIWGFSVPRLLGISDRLEAQPVEGSDKPKIVPNTLRVGRGQLKVVSTGFLVGEDTALM
                     WRGLMLAKAVEQFLRDVAWGEMDYLLIDMPPGTGDVQMGLARMLPRTDMIIVTTPAQA
                     AQKVA"
BASE COUNT          655 a         1383 c         1423 g          636 t
ORIGIN      
        1 cgcatcgagg tcgcggcgcg gctcgaagcc tgggcgatca gccccagctg gggtctcgac
       61 gagcgcttcc cgatgttcga acaagagatc gaatacggcg gtgcgagctg ggaaatcggc
      121 gaatgccccg acgactgacc gcggtctgac cgacgtttgg catcggtgga cgggagctgc
      181 gggggtacgg tcggggtcgt ggagttgatc gcttcggggc gggactgtga cgtgttcgat
      241 ctgggagatg gcacggtgct gcgccgccgc cgcgaccccc gctcggtcga accgcaagcc
      301 gccgtgatgc atcacgtcgt cgcccacggc tacccgtgcc cggcgccgat ccgcgcgcag
      361 ggaccggaca tggtnnnnnn nnnnctggaa ggcccgaccc tgctggcgca actgctcgcc
      421 ggcgccgacg aacaggctct acgcgacgcg ggcgcgctgc tggccgagtt gcacgaccgg
      481 ttgcaccgta tccccaccat gggtggcgga ccgggcacga tcgtgcacct cgacctgcac
      541 cccgacaacg tgatgatgac ccccgacggg ccgatggtga tcgactgggc caacggggcc
      601 gacggcccgg ccgaactcga cgtggcgatg acgtgggtcg tgatggagcc ggtcgtgcag
      661 gtgttccccg aggcgcgccc gctgctcgac ggcttcctcg acgcggccgg gcgcgacctc
      721 gcccaccgtg gcttacgcgc cgcctgcgat cgccgcctgg ccgaccccaa cgccaccgag
      781 tccgaacgtt ccgcgatcgc caccctccgc gccaccgcca catagcgcgc caccaggtcc
      841 gcccgcggtg aatccggctt ccgcggagta ccgagttacc ggattccggc aaaccnnnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnngcgcga gggcggtgca gggggcgtgg cgccagcagt
      961 cggcgacgtg gtcgttgacc aagccgcagg cctgcatgta ggcgtagcag atggtggtac
     1021 cgacgaagcg gaagccgtcc ttcttcatcc gcttgctcat cgcatcgctg acgggggtga
     1081 agccgggaac atcggccatc gtgatcgcgc cggtctggat cggcacaccg cccacgttgc
     1141 tccagatgta gtcggccggg tcggccaggc tttgccagat gcgggcgttg gtgacggtcg
     1201 aggtgatctt gccgcggttg cggatgatgc gcgtgtcgag catgcgggcc gcgacatcgg
     1261 cctcggtgta cgcggcgatg cgggcgatgt cccactggtc gaaggcgtcg cggtagccgt
     1321 ccttgcggag caagatcgtg tgccaactga gcccggcctg cgcgccctcc agggtgagat
     1381 actcgaacag cgtcaccggg tcgtactgcg gcacacccca caccgtgtcg tgatagtgcg
     1441 cctcctcagc atgatgcgtc gcccacccgc agcgagtggg ctcgctgagc tcgctcattg
     1501 gggccgatag gcccaggctt cgatttcgac ggaggcgccg gcgaggggga ggccggcgac
     1561 accgacggtg ctgcgcgccg ggcggtggtc gccgaagccc gcgacgtagg cctcgttgaa
     1621 cgacgcgaac gtgtcgacgt cggtgagaaa gcacatcgtc ttgaccacat cgctcaacgc
     1681 cgcgccgacg gtggcgagtt gggcggcgat gttggcaaca gcggcggtcg tctgcgcggc
     1741 gacaccaccc tcgaccagcg tgccgtcggt gacaccgagt tgaccggaca cgatgatcca
     1801 gtcgccagcg cgcaccaccg gggtgtaggg accgacaggc ttgggagcag acgcattgga
     1861 catgcccgcc actgtagaac cccgaacgct ttgtagattc ggcgagcttc acaactagcg
     1921 tcgaccgcat gatccccgtc gatcgccgga cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnncggc tcgatttccg cgggacctgt tggtgccggg gcggcagcgg
     2101 ttggtgtact cgctggtgcg cgacggcgag ctccaggccg acgggccgga cacgctcacg
     2161 ggcaagatcg tccgcaacga cgacaacgcc gtcgtcgtgg cctcgatcag cgccagcaaa
     2221 cggcgcatcg atgccaacct gaactactgg gacttccaca ccgagttgag cgacacgggc
     2281 atctacacgc tgtacgtgga cggggccagc gccgacggca cctcgannnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnccc gcgcggtgtg
     2401 acgccgatct gcacccgctc gcccgagccg tgcccgttcc acgaccagac cgtgaccgag
     2461 gcgctcgccc tcggcaagcc ggtcgcctac atgatcggta ctcccgcgca ctgccagttc
     2521 ggcacgtgcg ctccgggcat ggagtttctc atcgccgcgt cacagcggct cggcgactcg
     2581 ctcgccgtcg tgcacgccga ggtctacacc gacgactcgg cgacggtggc cgccccggcg
     2641 atgaacgagt actcgctggt gttcgaaccc gtgatctacc tggccgacgc cacgggcacg
     2701 atcgtcaccc gtcttgacgg gatctggaac cagggcgaac tcgacgaagc actcgacgcg
     2761 ctcgtcgcct gaacccgagt cgccgtcgcc cagacctgac cgccggacgc gcaacgacgg
     2821 cggggtgtcc cgccgtcgtc atcgtcgttc tggaagaggt cgggtcagct gaagctctga
     2881 ccgcagccgc agctacgggt ggcgttcggg ttggtgatgt tgaacccggc ctggttgagc
     2941 ccgtccttgt agtcgagcga ggcaccttcg agcagctggg ccgactgagc gtcgacgagc
     3001 acccggacgc cttcgccgaa ggtggcctgt tcgtcgtcgg cggcgatgtc gccgtcgaag
     3061 tacatctcgt acgagaagcc gctgcaccca ccggggcgca cggcgacacg cagcgcgagg
     3121 tcggtggcgc cttcggcctc caacagctcc ttcaccttgc tggcagcgtt gtcggtcaac
     3181 gtgatcatct gcgaatccct cctcggaagt tgcggtgacc catcgtagcg gggggttcca
     3241 cgatgggttg tcgctggtgt gcggggtctc gacgcggatg cgccgacggg caaggcaaca
     3301 cggcaacccc gggtagtatt ccgtatggcc tcgcagcaac ccagcgtggc gcccagcccc
     3361 gacgagatcg tgcagctctt gcacgccgtg atcgaccctg aactgggtgc cgacatcgtc
     3421 accctgggta tggtgcccga cgtcgcggtg tccgcacccg gtgccgacgg tctcgtcgac
     3481 gtcacggtcg cggtcaagct caccatcggc ggctgcccgc tgcgggcaca gatcaagaaa
     3541 gacgtcgaga gccgaatcgc gctgcaccca gcggtgcgcg acgtgcacat cgaatggggc
     3601 gagatgacgc ccgacgagcg cagcgaggtg atgaccaagg cccgctggaa cgcccgcgag
     3661 cacgcgcccg acaccgaggt ccccgcgtcc acccgcatcc tggcgatcgc cagcggcaag
     3721 ggcggcgtcg gcaagagcag cgtcacggtc aacctcgccg ccgcgctggc cgcgcagggc
     3781 ttcaccgtgg gcgtgctcga cgccgacatc tggggctttt cggtgccccg cctgctgggc
     3841 atcagcgacc ggctcgaggc gcaacccgtc gaggggtcgg acaagcccaa gatcgtgccc
     3901 aacaccctgc gggtcggacg cggccaactc aaggtggtgt cgaccgggtt cctggtcggc
     3961 gaggacacgg ccttgatgtg gcgtggcctc atgctggcca aggccgtcga gcagttcttg
     4021 cgcgacgtgg cctggggcga gatggactac ctgctgatcg acatgcctcc cggcacgggc
     4081 gacgtgcaga tgggcctggc tcgcatgctg ccgcgcaccg acatgatcat cgtcaccacg
     4141 cctgcgcagg cggcgcagaa ggtcgcgtag agccgacggc ccgactcggg atcgggtgcg
     4201 ccctcgtccg tctcttcgac gggacggagc ggggccgaca tcggcccggt gaagccgcgt
     4261 ccgtcgagga ggcggttgag cgtgtagaag gcgagcggat tt
//
LOCUS       DAORNF010000228         4286 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_15327_length_4286_cov_1.756795, whole genome shotgun sequence.
ACCESSION   DAORNF010000228 DAORNF010000000
VERSION     DAORNF010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4286
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15327_length_4286_cov_1.756795"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(239..379)
                     /locus_tag="PLV68_09975"
     CDS             complement(239..379)
                     /locus_tag="PLV68_09975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01617.1"
                     /translation="MIAYEAAGHEFARADQLSCGGLDEMLGVPAPRLIVNGTEISPGC
                     HG"
     gene            421..1275
                     /locus_tag="PLV68_09980"
     CDS             421..1275
                     /locus_tag="PLV68_09980"
                     /inference="COORDINATES: protein motif:HMM:NF006140.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HRE01618.1"
                     /translation="MSDYETIIYEEPADKVARIVLDRAEARNAQSMHMLYELNDAFDR
                     AAQDDSISVIILAANGPHFSAGHDLRDRDGRQVVAEHRRIGTWCGFGCVGAESQMAIE
                     KEMYVGLSERWRNIPKPTIAAVQGKVIAGGLMLVWPCDLIVASQDALFQDNTVSMGVS
                     GAEFFNHPFEVGVRKAKEMLFTSDFLTADEVHRLGMVNHVVPLDELESFTLQLASRIA
                     EKPLFALKLAKEAVNVAQDNQGRLSSMQTSFAYHQLCHSHNQQVHGALIDPSFMAKTF
                     GAKREAES"
     assembly_gap    1294..1307
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1329..2753)
                     /locus_tag="PLV68_09985"
     CDS             complement(1329..2753)
                     /locus_tag="PLV68_09985"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRE01619.1"
                     /translation="MAIIGCGVGGIATAVKLHKAGIDTYTIFEQSDGPGGTWWDNRYP
                     GCEVDVPSQAYSFSFQNHDWTRTHASSTELQGYIEGVVDRFGVREHIRFSTGVASVVW
                     DAPACRWHLTTTTGEKHDFDVVVSAVGFLNVPRYPTWPGLDTFTGPRFHTARWEEHDL
                     SNQRVAIVGTGSTAAQVVPAIADRVGELLVFQREPGWVLPKHERDFTPAERARNPIRH
                     KLDRAKLFYKAIAINRVYDPDSDEHKKFEAVGLKYIARTIADPAVQQAVTPSHPYGCK
                     RPVLATTFYPALNKPNVRLVPRAVVEVTPSGVVDADGTELDVDVLVYATGFHANDFLA
                     TFEVHGMSGTLREAWAERARAYLGITVPGFPNFFIQYGPNTNGGWSAIAQLERQAEVA
                     VRGVKRLRRGWVRSIDTRPRALDRWVRFVDRNIAEHAPVMDGPCNNYFHSESGANVTQ
                     WPLSHARYYVMTKVMPFFGLVGRR"
     gene            complement(2961..>3543)
                     /locus_tag="PLV68_09990"
     CDS             complement(2961..>3543)
                     /locus_tag="PLV68_09990"
                     /inference="COORDINATES: protein motif:HMM:TIGR03564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HRE01620.1"
                     /translation="KPVRHMSEYLSILSDLLATGSADVEGHAWSYIGTDTRPSAGVPK
                     IMIAALGEQMLKLAGRRSDGTILWCVGPKTIRTHIAPIINDAAAAADRPAPSIVCSIP
                     VWVTDDPAPARAFLAQVLAAYATLPSYRAMMDVEGVAGLGDLSIVGNEAFVRDAIGAI
                     AESGATDFTPVPMGANPDEEARTLQVLTEAMRS"
     assembly_gap    3544..3553
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3554..3884)
                     /locus_tag="PLV68_09995"
     CDS             complement(<3554..3884)
                     /locus_tag="PLV68_09995"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HRE01621.1"
                     /translation="MRLGINGSGRLLTHGVAGVLDDITRSEERGFSSYWVAQIGLIDA
                     LTLIGAHGATGSDMAIGTAVISTWERHPHMLAAQALTTQALVGDRLIVGIGVNHQPHV
                     EASLRLTW"
     gene            complement(3981..>4286)
                     /locus_tag="PLV68_10000"
     CDS             complement(3981..>4286)
                     /locus_tag="PLV68_10000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01622.1"
                     /translation="RELVGWIRPEGDAWVAVSILGRAASGPVEWVEAERTLEEHGLSL
                     LAGVWTLRSGDGPIDVRIVQVALDGIVVQTDDFGAIDVAVERFDLPWPAPAELQPRP"
BASE COUNT          701 a         1384 c         1462 g          715 t
ORIGIN      
        1 cgaggtcttc ccgcttccag cgtcaccaac gtccgttggc tgatcccggc tcgggcggcg
       61 agttcgtgct gggtccaacc gcgctgctga cgcgctcgcc gaatggaagt gcccacttcg
      121 ttgaccgtcc tgaccctcat cgatatgagc gtacgttcat aatcatgaat atgcaagtac
      181 cttcatttcc atgaatgtga gcgcacctgc acctggcagg gcaggggcag agcatcgctc
      241 agccgtggca gccgggggag atttcggtgc cgttgacgat caggcgaggg gcggggacgc
      301 cgagcatctc gtccaggccg ccgcaggaga gttggtcggc gcgggcgaac tcgtggccgg
      361 cggcctcgta ggcgatcacg tcctgctgtt tcgatgatcg ctagtagctt cgttcgatct
      421 atgagcgact acgagacgat catctacgaa gagccggccg acaaggttgc caggatcgtg
      481 ctcgatcggg ctgaggctcg caacgcgcag agcatgcaca tgttgtacga gctcaacgac
      541 gcgttcgatc gcgccgcgca ggacgactcg atcagcgtga tcatcctcgc cgccaacggg
      601 ccgcacttct cggccggcca cgacctgcgt gaccgtgacg gccgccaggt cgtcgccgag
      661 caccgccgga tcggtacgtg gtgcggcttc ggctgcgtcg gcgccgagtc gcagatggcg
      721 atcgagaagg agatgtacgt cgggttgtcc gagcggtggc ggaacatccc caagccgacc
      781 attgccgcgg tgcagggcaa ggtgatcgct ggtgggctga tgctcgtgtg gccgtgcgac
      841 ctgatcgtcg cctcgcagga cgcgctcttc caggacaaca cggtgtcgat gggtgtcagc
      901 ggcgctgagt tcttcaacca cccgttcgag gtcggggtcc gcaaggcgaa ggagatgctg
      961 ttcacgtccg acttcctcac cgccgacgag gtgcaccggc tcggcatggt caaccacgtc
     1021 gtgccgctcg acgagttgga gtcgttcacg ctgcagctcg catcacggat cgccgagaag
     1081 ccgctgttcg cactcaagtt ggccaaggaa gcggtcaacg tcgctcagga caaccagggt
     1141 cggctgagct cgatgcagac gtcgttcgcg tatcaccagc tctgccacag ccacaaccag
     1201 caggtccacg gcgcgctcat cgacccctcg ttcatggcca agacgttcgg tgcgaaacgg
     1261 gaagctgagt cgtagacgtc gcctggggcc gcgnnnnnnn nnnnnnncgg cgaggacctg
     1321 cagcgcggtc agcgtcggcc taccaggccg aagaagggca tcaccttggt catcacgtag
     1381 tagcgggcgt ggctgagcgg ccactgggtc acgttcgcgc ccgactcaga gtggaagtag
     1441 ttgttgcacg ggccgtccat cacgggagca tgctcggcga tgttgcgatc gacgaaccgg
     1501 acccaccgat cgagggcgcg cgggcgggtg tcgatcgacc gtacccagcc gcggcgcagc
     1561 cgcttcacgc cgcgcacggc cacctcggcc tgccgttcga gctgggcgat cgcgctccac
     1621 cctccgttgg tgttcggacc gtactgaatg aagaagttgg gaaaccccgg cacggtgatg
     1681 ccgaggtagg ccctggcgcg ctcggcccac gcttcgcgca acgtgcccga catgccgtgc
     1741 acctcgaagg tggcgaggaa gtcgttggcg tggaacccgg tcgcgtagac gagcacgtcg
     1801 acgtcgagct cggtgccgtc ggcgtcgacc accccgctcg gcgtcacttc gacgacggcg
     1861 cgaggcacga gtcggacgtt gggcttgttc agcgccgggt agaaggtggt ggcaaggacg
     1921 gggcgcttgc agccgtaggg atgggacggc gtgacggcct gctgcaccgc tggatcggcg
     1981 atcgtgcggg cgatgtactt cagtccgacg gcctcgaact tcttgtgctc gtcgctgtcg
     2041 gggtcgtaga cgcggttgat cgcgatggcc ttgtagaaga gcttggcccg gtcgagcttg
     2101 tggcggatcg ggttgcgggc acgctctgcc ggcgtgaagt cgcgctcgtg cttcggcagc
     2161 acccagccgg gctcgcgttg gaacaccagg agctctccga cccgatcggc gatcgccggc
     2221 accacctgtg cggcggtcga accggtgccg acgatcgcga cccgctggtt gctgaggtcg
     2281 tgctcttccc atcgcgcggt gtgaaaccgc gggccggtga aggtgtcgag ccctggccac
     2341 gtcggatagc gcggcacgtt gaggaacccc accgctgaga cgacgacgtc gaagtcgtgc
     2401 ttctcaccag tggtcgtcgt gaggtgccag cgacaagcgg gagcgtccca gacgacggag
     2461 gcgacaccgg tagagaagcg aatgtgctcg cgcacgccga agcggtcgac gacaccttcg
     2521 atgtagccct gcaactcggt gctgctggcg tgggttcgcg tccaatcgtg gttctggaac
     2581 gagaacgaat acgcctggga ggggacatcg acctcgcagc ccggatagcg gttgtcccac
     2641 caggtgccgc cggggccatc ggactgctcg aagatcgtgt acgtgtcgat gcctgccttg
     2701 tgcagcttga ccgcggttgc gatgccaccg acaccgcagc cgatgatcgc cacgtgcggc
     2761 gagtgccgtc ggccgcgctg tccaacctgt tgtcgcgtcg tcgctcggcc catcctgatc
     2821 accgcacctc ccagagaacg tctaccaaac gttcgttgta gaacctaacc gatgcggcat
     2881 gggcctggga gatcccgcgt tggtggcagt tcgagtgcgt gggcggtcag acggacgacg
     2941 ccagggcgtc tgacggaccg ctatgacctc atcgcctcgg tgagcacctg gagggtccgc
     3001 gcctcctcgt cggggttggc ccccatcggc accggggtga agtccgtggc tcccgactcg
     3061 gcgatcgctc cgatcgcgtc gcggacgaac gcctcgttgc cgacgatcga gaggtcgccg
     3121 agcccggcga cgccttccac gtccatcatc gcccgatagc tggggagcgt ggcgtacgcg
     3181 gcgagcacct gggccaggaa cgcccgggcg ggcgcaggat cgtcggtgac ccagaccggg
     3241 atcgagcaga cgatcgacgg tgccgggcga tccgccgcgg cggcggcgtc gttgatgatc
     3301 ggggcgatgt gcgtccggat cgtcttcggc ccgacgcacc acaggatcgt gccatcgctg
     3361 cggcgaccgg cgagcttcag catctgctca ccgagcgcag cgatcatgat cttcgggact
     3421 cccgccgagg gccgcgtgtc ggtgccgatg taggaccagg cgtggccctc gacgtcggcg
     3481 gaaccggtcg cgagcaggtc ggacaggatc gacaggtact cgctcatgtg gcggaccggc
     3541 ttgnnnnnnn nnncccacgt gagccgcagg ctggcctcga cgtggggctg gtggttgacg
     3601 ccgatgccga cgatcagccg atcgccgacg agggcctggg tggtcaacgc ctgggccgcg
     3661 agcatgtgcg gatggcgctc ccaggtggag atcaccgccg tcccgatcgc catgtccgac
     3721 ccggtggcac cgtgcgcgcc gatgagcgtc agtgcatcga tcagcccgat ctgggcgacc
     3781 cagtaactgg agaacccacg ttcctcggac ctggtgatgt cgtccaacac cccagctacc
     3841 ccgtgcgtga gcaaccgtcc cgacccattg atccccaagc gcatgtcgcc aacctacgtc
     3901 gccatcgcca ccgacgaaaa gggtccgggg gaccaggcgg tccgggtggc ccggggatgc
     3961 ggtgacccga ccagggggat tcagggtcgg ggctggagct cggctggggc cggccatggc
     4021 agatcaaacc gctcgaccgc gacgtcgatc gccccgaagt cgtcggtctg caccacgatg
     4081 ccgtccagcg cgacctgcac gattcgtacg tcgatcggac cgtcgcccga cctcagggtc
     4141 cagactccgg cgagcaagct caggccgtgt tcttcgaggg tccgctccgc ctcgacccac
     4201 tcgaccggtc ccgatgctgc ccgcccgagg atcgacaccg cgacccaggc gtccccctcg
     4261 ggacgaatcc accccaccag ttcgcg
//
LOCUS       DAORNF010000229         4276 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_15379_length_4276_cov_1.714286, whole genome shotgun sequence.
ACCESSION   DAORNF010000229 DAORNF010000000
VERSION     DAORNF010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4276
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15379_length_4276_cov_1.714286"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    244..298
                     /estimated_length=55
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <299..1104
                     /locus_tag="PLV68_10005"
     CDS             <299..1104
                     /locus_tag="PLV68_10005"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRE01623.1"
                     /translation="RAADAPPDIQDAKAAELVASILEQHHGDVAAVPVAWYIGHVPAT
                     DANEWDTIPHPDAGNTLTPREYQTRWLAKHAELLAAAPDDTAAGQLAAPSPGSCFGGS
                     ITALDGQWALPGPRELIDANPDALTAPHHDYPAWDWIIPTNTPIYAVRGGTVTSVKQW
                     PHNWWTQGCARNSTGCQTCGIGLTITDPDGNRWTYCHATNLTTTLGADVTAGQQIMWS
                     GNTGRSGTPHLHLELRTPDNQRHCPQPVLVAIAGGSAPAALQNAPTCWF"
     assembly_gap    1140..1240
                     /estimated_length=101
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1272..>1518
                     /locus_tag="PLV68_10010"
     CDS             1272..>1518
                     /locus_tag="PLV68_10010"
                     /inference="COORDINATES: protein motif:HMM:NF021640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide-binding protein"
                     /protein_id="HRE01624.1"
                     /translation="MANDAPAPSPADPRKVFVVHGRNALARRGLFEFLRALDLDPIEW
                     SEALHMTGQASPYIGNVLDAAFGAAQAVVVLQTPDDVA"
     assembly_gap    1519..1574
                     /estimated_length=56
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1575..2167
                     /locus_tag="PLV68_10015"
     CDS             <1575..2167
                     /locus_tag="PLV68_10015"
                     /inference="COORDINATES: protein motif:HMM:NF021640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotide-binding protein"
                     /protein_id="HRE01625.1"
                     /translation="NVLFEAGMAMGRNPERTVIVELGQVKSFSDIHGRHVVRLDNSVA
                     QRQALANRLQTAGCAVKLTGTDWHDAGDLTPPAAPGGGLPLGRKLPSSQASGLPRLEA
                     RLRSNGSGKMDELVITNHGPGDVYELGYEAPESAAMMMRRDDGFPVPKLPPGKSVRAW
                     RSQTLSHGNEPYFTVVLTGRTADGTEITQEEFVSGG"
     gene            2358..>2541
                     /locus_tag="PLV68_10020"
     CDS             2358..>2541
                     /locus_tag="PLV68_10020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01626.1"
                     /translation="MQVDYMDEAVAPAIVQFMDNAIGEVFKGASTKHVKFERTGHLDV
                     PGHPDPLTEPDDMVIVS"
     assembly_gap    2542..2551
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2763..3149)
                     /locus_tag="PLV68_10025"
     CDS             complement(2763..3149)
                     /locus_tag="PLV68_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01627.1"
                     /translation="MGVVVDNDLRAEAYGRLLEEFPGASRLLSASAAAFWCGHDTAGQ
                     VKYAALQTEAGLQVAPYIYMGDFEAGSTMDLAAAMRRCVHDGAVFPIAEGTDEADDIS
                     LPDLRPWTFTVQIPDRAETLTSVPHS"
     gene            complement(3879..4076)
                     /locus_tag="PLV68_10030"
     CDS             complement(3879..4076)
                     /locus_tag="PLV68_10030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01628.1"
                     /translation="MTTIEIDYSRYGRSTRITVPLFQRERNIEIGERVRVIGDDVPTR
                     TATVADLIDNGRKAILDLDPA"
BASE COUNT          724 a         1426 c         1268 g          636 t
ORIGIN      
        1 cccgccgaac agtggaaatc agccgccgag ttccacccgc aacaccgctc gcgtcccgta
       61 cggggagatg aaagccgcgc tcagtgccac cgtcgccgtc ctcgtcggcc tcaccatgct
      121 ccccgcactg ctcaccaacg gcgacccgcc cgcgctcgca gtgtgcggca tccacgccgg
      181 ccccaccagc gtcgtgctcg ccaccatccg caccctcgaa tcaggcggca actacaccgc
      241 ccannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncc
      301 cgcgccgccg acgcaccccc cgacatccaa gacgccaaag ccgccgaact cgtcgccagc
      361 atcctcgaac aacaccacgg cgacgtcgcc gcagtccccg tcgcctggta catcggccac
      421 gtccccgcca ccgacgccaa cgagtgggac accatcccgc accccgacgc cggcaacacc
      481 ctcaccccac gcgaatacca aacccgctgg ctcgccaagc acgcggaact cctcgctgcc
      541 gcacccgacg acaccgcagc cggccaactc gcagcaccgt cgcccggcag ctgtttcggc
      601 ggatcgatca ccgccctcga cggccaatgg gcgctccccg gcccacgcga actcatcgac
      661 gccaaccccg acgcgctcac cgcaccccac cacgactacc ccgcctggga ctggatcatc
      721 cccaccaaca cgcccatcta cgccgtccgc ggcggcaccg tcacctccgt caaacaatgg
      781 ccccacaact ggtggaccca aggctgcgcc cgcaactcca ccggctgcca aacctgcggc
      841 atcggcctca ccatcaccga ccccgacggc aaccgctgga cctactgcca cgccaccaac
      901 ctcaccacca ccctcggcgc cgacgtcacc gccggccaac agatcatgtg gtccggcaac
      961 accggccgat ctggcacgcc ccacctccac ctcgaacttc gcacccccga caaccaacgc
     1021 cactgccccc aaccggtgct ggtagcgatc gccggaggat ctgctcccgc tgccctgcaa
     1081 aacgcgccca cctgctggtt ctgacgaacg ggatacgacg gtagtgctgc gattccgccn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ccttgtcaca cccgtcgcgt
     1261 acgctccggg catggctaac gacgcgccag cgccatcgcc ggcagatccg cgcaaggtgt
     1321 tcgttgtcca cggccgcaat gcactcgccc gtcggggtct attcgagttc cttcgtgcgc
     1381 ttgatctcga ccccatcgag tggagcgagg cgctgcatat gaccggtcag gcctcgccgt
     1441 acatcggcaa cgtgctcgac gcggccttcg gcgccgcaca ggcggtcgtc gtgctgcaga
     1501 ctcccgacga cgtcgcccnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnncgaacg ttctgttcga ggctgggatg gcgatgggcc gcaacccgga
     1621 gcgcaccgtg atcgtggaac tcggccaggt caagtcgttc agcgacatcc acggccggca
     1681 cgtcgtccgg ctcgataact cggtcgctca gcgacaagcc ctcgccaacc ggctacagac
     1741 ggcggggtgt gccgtcaagc tgactggcac cgactggcac gacgccggcg atctcacccc
     1801 gcccgctgca cccggcggcg ggctcccgct tgggcgcaag ctgccgtcgt cgcaggcctc
     1861 tggcctgccg cggctcgaag ctcggctgcg ttccaacggc agcggcaaga tggacgaact
     1921 ggtcatcacg aaccatggcc ccggcgacgt ctacgaactc ggctacgagg cgccggagtc
     1981 ggcggcaatg atgatgcgac gcgacgacgg cttcccggtg ccgaagctgc cgcccggcaa
     2041 gtcggtcagg gcgtggcgaa gtcagacgct ctcccacggc aacgagccgt acttcacggt
     2101 ggtcctcacc ggacgcaccg cggatggcac cgagattacg caggaagagt tcgttagcgg
     2161 aggttgacat ggacatagaa gtgcagctcc cgctcgacag tgatggcttc ctccggcgcg
     2221 aatgcccgca ctgcttcgag cagttcaagt ggcatcacgg tccggccaac gaggaggcgg
     2281 aatcggctgc gcatccaccg gcgtatcact gtcccttgtg cggccagccg gctggacacg
     2341 agtcgtggta cacacagatg caggtcgact acatggacga ggcagttgcg ccagcgattg
     2401 tgcagttcat ggacaatgcg atcggcgagg tcttcaaggg tgcctcgacg aagcacgtca
     2461 agttcgagcg gacagggcac cttgatgtac caggccatcc ggacccgctc acggagccgg
     2521 acgacatggt catcgtgtcc tnnnnnnnnn ngggtacgaa ccggtgaagg ttcctgccga
     2581 gcacaccggc ccgctctact gcttgatctg cggcaccgcg ttcgcggtgt agtggtggcg
     2641 atccgcactc cacagcgctt ccggtccggc agaagggtcg gccggccatc ggtagcgctc
     2701 gaccggccgg atcttcgaac aagcgaagct ggcagcggac gcgattgcca tcgccacacc
     2761 ggtcagctgt ggggaaccga cgtcagagtc tcggcacggt ctgggatctg gacagtgaag
     2821 gtccatggcc gaagatccgg aagggagatg tcgtcggcct cgtcggtgcc ttcggcgatc
     2881 ggaaacactg caccgtcgtg gacgcagcgg cgcattgccg ccgctagatc catcgtgctg
     2941 ccggcctcga agtcccccat gtagatgtag ggggcgacct gcaagccggc ctccgtttga
     3001 agcgccgcgt acttcacctg gcccgcggtg tcgtggccgc accagaacgc agcggccgac
     3061 gcgctgagaa ggcgcgaggc accgggaaac tcctcaagca agcgcccgta cgcctctgcc
     3121 cgcaggtcat tgtcgacgac gacgcccaag cccgtcgcga gaacttcctc gatgcggcag
     3181 tcgctgcgac tcttgcctga ccagtagtcg acgaaccagt cgctggttcc agagcccggt
     3241 gatccattca ctgtcgactc tcgtcgagtc cgtaggccag aaatgaacct ctgagaccgt
     3301 cacgtttcgc cagtcccagc gttcgatcgc acgacgcgct gtaccagggt cgggaaacag
     3361 atacacgcag tcgagacggg atacggctgt cggggtggtg gatcgacgga caagctcaag
     3421 ggtcatctca tcccgatacg tttgcgtcat accggtgagc gcgagtcgtt ccagcacccg
     3481 gtatgtccag gcggagatgc cgaaaccctc agcagatccg ggcacgtgcg aagagacccg
     3541 cccgacagca atctcccaaa gcgtgagcgg gttcgccagg ttgaacagcc cgaagagaac
     3601 ctctggttca cgcccgcgcc gctccgtccg aatgtcgatg ggtgtgtatg cgtcggtcgt
     3661 cactgctcgt tccgctgact cgcgaggtcc taacagcagc gtacgcgtcg ggggcatgcc
     3721 aaaggttccg tgcgcggacc cgacggttcg gtgcaacgcg tgaacgcggc gcgatgaccg
     3781 gcgagggtgc actgcatgtg ccctcagagt gccgtctggc gacgcagaga tggcgggtcc
     3841 cgatgcgatt cgctccccgc ctgggggctc gccgccgttc aggcagggtc gaggtcgagg
     3901 atggctttgc ggccgttgtc gatgaggtcg gcgacggtgg cggtgcgggt ggggacgtcg
     3961 tcgccgatga cgcggactcg ttcgccgatc tcgatgttgc gttcgcgttg aaacagcggg
     4021 acggtgatcc gggtggaccg cccgtaccgg ctgtagtcga tctcgatggt ggtcatcgtc
     4081 ctgggcatag cgaactgacc gtgattctac ttgggctgcc gggggctgaa cagggcgagg
     4141 agccgatggg cgtggggttc gaggtgtgcc cgggcggcgt gttcgaggtc ttcgtcatcg
     4201 ggcgggtcga cggctcggag tcgggggtcg tcgagtggtg ggagggcgat gtcgtagtgg
     4261 acggactgga tcgcgg
//
LOCUS       DAORNF010000230         4274 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_15391_length_4274_cov_1.625267, whole genome shotgun sequence.
ACCESSION   DAORNF010000230 DAORNF010000000
VERSION     DAORNF010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4274
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15391_length_4274_cov_1.625267"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>238)
                     /locus_tag="PLV68_10035"
     CDS             complement(<1..>238)
                     /locus_tag="PLV68_10035"
                     /inference="COORDINATES: protein motif:HMM:NF012885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HRE01629.1"
                     /translation="TNPLQREVVFGLDKDGIVDIAVNAAKMARKLEATIPETEVRYEY
                     SPESFTLTEPQFALDICHAVMDVVEPTPERKVIFN"
     assembly_gap    239..299
                     /estimated_length=61
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<300..655)
                     /locus_tag="PLV68_10040"
     CDS             complement(<300..655)
                     /locus_tag="PLV68_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HRE01630.1"
                     /translation="MPPHNVTPKKMPFEKYQRWLPLTLADRTWPDAIITTAPLWCSVD
                     LRDGNQALIDPMDPERKRRMFDALVKMGFKEIEVGFPAASQPDFDFVRQLIEEDLIPD
                     DVTIQVLVQCRQELLER"
     gene            1190..>1544
                     /locus_tag="PLV68_10045"
     CDS             1190..>1544
                     /locus_tag="PLV68_10045"
                     /inference="COORDINATES: protein motif:HMM:NF028519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HRE01631.1"
                     /translation="MPTRLLVVMATLAATVVTGLTLTASPASAAALITGTVIDDANAD
                     GVVNTALGTGAEVGLAGVTVELVDDLGNVLDSAITDAMGAYSLDADGYEGDARIRVTT
                     PAGWVVGTSGAGSITR"
     assembly_gap    1545..1810
                     /estimated_length=266
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1811..>3648
                     /locus_tag="PLV68_10050"
     CDS             <1811..>3648
                     /locus_tag="PLV68_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01632.1"
                     /translation="VRSADNVAFNFAISGSAADDSVGSVANVIFQQTITPSDPGALIN
                     FVRIPTICTPGNGGGTPPPSSRIEYWDPGTSSWVEYAGPSTGYLVPDVPAAGIGQPLR
                     LTCNKGTYTTGDAATLGTTVHIDGVATNGSSFASEAVLFAVDPNGAAIGVADGPLGGP
                     DINISSRPEWDLRKGGFYRQDWTTADPDGAGPMQREAGFYTYYNIMIGADRKAGNEPL
                     EQPIVLTDQLSFFDNAGNPVTLVAGVDYYVNPTCLDFTPAGLGAYAGQVISRASAVVN
                     PYTTAQAAVNSGTCTFDNATGEITWSGIDFGGPYPTATTSGQSLASGPYLVANKWLRV
                     WMPYSTLDNMFGDDPDNDAGSGWLWNRVDYTTTPLGVSGTPNYAGQPEPGDCDLSSPI
                     DPTADPTPTGCDPLPAPDLLTGQARSNNIAGPNSFTFSPGTWTKYLFKPNGNNRQYLL
                     HTDMSANHDGAGTVQPGEYTSTWLTWVSNSRTYENPMQCDVWDRTMYELVTLAEANVG
                     AVSGSPTPYATVTGANTNAALLTFEFASIPIDYDTDGAGPDTGDDPLGGGFNPGTGRY
                     GGTWSSQAAAVAAGCMPGAEDVTWVTDPTTLPGGAASANAVRVSGP"
     assembly_gap    3649..3678
                     /estimated_length=30
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3679..>4274
                     /locus_tag="PLV68_10055"
     CDS             <3679..>4274
                     /locus_tag="PLV68_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01633.1"
                     /translation="PTMGPGSDRLLRVGFKVRNTFDGGPHNGQAIPVGAIGANYGNVS
                     GVSGTFPANGYNPVVDAGLSGDRLTWTRAVLGVKKLTVAVDGMCDAPCAQPIDVNGSV
                     LAGKPIIWEIRPVVNAGSPTPAPVNNLTLTDVLPVYVEYDDACTAALTGGTPADDVLF
                     NTPAAGQTTLVWNVGTVIPNTEITPYRICTQTDPIAPAP"
BASE COUNT          663 a         1315 c         1294 g          645 t
ORIGIN      
        1 ggttgaagat gaccttgcgc tcgggggtcg gctcgaccac gtccatcacg gcgtggcaga
       61 tgtcgagcgc gaactgcggc tcggtcaggg tgaagctctc cggcgagtac tcgtagcgca
      121 cctcggtctc ggggatggtc gcttccagct tgcgggccat cttggccgcg ttgaccgcga
      181 tgtcgacgat gccgtccttg tcaaggccga acaccacctc acgctggagc gggttggtnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc
      301 gctcgagcag ctcttgacgg cactgcacca ggacctggat cgtgacgtcg tcggggatca
      361 ggtcctcctc gatcagttgg cggacgaagt cgaagtcggg ctgactggcg gccgggaagc
      421 cgacctcgat ctccttgaag cccatcttga ccagcgcgtc gaacatccgc cgtttgcgct
      481 cggggtccat cggatcgatc agcgcttggt tgccatcgcg caggtcgacg ctgcaccaca
      541 gcggcgctgt ggtgatgatg gcgtcgggcc aggtgcggtc ggcgagggtg agggggagcc
      601 agcgctggta cttttcgaag ggcatcttct tgggggtgac gttgtgcggt ggcattgcgg
      661 gagctccctt atgagcggtg atggtgagcg gtggtgcccg gtgggcggtg ggtcgggtgg
      721 cggtgatgac gggctcgcga tcgggccgga aaaagcagaa cgcccctgcg ctgggcgcaa
      781 gggcgtgggc gaacgcgatc ttggggcgct cgccttacgt aagaaggagg aggccgatca
      841 gacgcgtcat cgggatcgac actagcaccg ctgcgcccga gcggctaccc cattcggacg
      901 aacgtgtgca acgggccgac agcacccctg acgtgatctt gcacgcatcg tgccgccccg
      961 atgcgtgcat tgccgaaccg aggtggtagt gtcgccaccg aattcatatc aggcgcggcg
     1021 gcgccgatcg agagggcccc tggtgttgcg cagcaacgcc ggtggcgttg gacgactctt
     1081 gatcggttcg ccggagacgg aatcagggcg gttaccacaa atgaatacga tgtcgaacga
     1141 ccgggcgcgt ccgtccggta cgcggtggac gccatcttgg cttccgaccg tgccaactcg
     1201 gctcttggtc gtgatggcca cgttggcagc aacggtggtc acaggcttga cgctcacggc
     1261 gagcccggca tcggcggctg cgttgatcac cggtacggtc atcgacgacg ccaatgcaga
     1321 cggtgtggtc aacaccgcgc tcggcaccgg ggccgaagtc ggcctggcgg gcgtcacggt
     1381 cgagttggtc gacgatctcg gcaacgtgct cgacagcgcg atcaccgatg cgatgggtgc
     1441 gtactcgctc gacgccgacg gctacgaggg cgatgcccgc atccgggtga cgacccctgc
     1501 tggttgggtc gtcggcacat cgggggctgg ctcgatcact cgggnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn cgtgcgcagc gccgacaacg tcgccttcaa cttcgcgatc agtggctcgg
     1861 cggccgacga cagcgtcggc agcgtcgcca acgtgatctt ccagcagacc atcacgccca
     1921 gcgatcccgg cgcgctgatc aacttcgtgc gcatccccac catctgcacc cctggcaacg
     1981 ggggcggcac gccgccgcca tccagcagga tcgagtactg ggaccccggc acctcgtcgt
     2041 gggtcgaata cgccggtccg tccacgggct atctggtacc cgacgtcccc gcggccggca
     2101 tcggccagcc gttgcggctc acgtgcaaca agggcaccta caccaccggt gatgcggcca
     2161 cgttgggcac cacggtgcac atcgacggcg tcgccaccaa cggatcgagc ttcgccagcg
     2221 aagcggtgct gttcgcggtc gatccgaacg gtgccgcgat cggcgtggcc gacggtccgc
     2281 tcggcgggcc ggacatcaac atcagctccc gtcccgagtg ggacctgcgc aagggcgggt
     2341 tctaccgcca ggactggacc actgccgatc ccgacggtgc cggtccgatg caacgtgaag
     2401 cgggtttcta tacctactac aacatcatga tcggtgccga tcgcaaggcc ggcaacgagc
     2461 cgctggaaca gccgatcgtg ctcaccgatc agctcagctt cttcgacaac gcggggaacc
     2521 ccgtcacgct cgtggcgggc gtcgactact acgtcaatcc cacctgcctc gacttcaccc
     2581 ccgccggtct cggggcgtac gccgggcaag taatttcgag ggcctcggcc gtcgtgaacc
     2641 cgtacaccac cgctcaggcc gccgtgaact cgggtacgtg caccttcgac aacgccaccg
     2701 gcgagatcac gtggtcgggc atcgacttcg gcggaccgta tcccaccgcg acgacgagcg
     2761 gtcagagcct ggccagcggg ccgtacctgg tggccaacaa gtggttgcga gtgtggatgc
     2821 cgtattcgac gctcgacaac atgttcggtg acgaccccga caacgacgcg ggatccggtt
     2881 ggttgtggaa ccgggtcgac tacacgacca cgccgctcgg tgtgtccggt acccccaact
     2941 acgcagggca gcccgagccg ggcgactgcg atctgtcatc gccgatcgat ccgaccgctg
     3001 atccgacacc gacgggttgc gatccgttgc cggcgcctga tctgctcacc ggacaggccc
     3061 gcagcaacaa catcgctggt ccgaacagtt ttacgttcag tccgggcacc tggaccaagt
     3121 acctgttcaa gccgaacggc aacaaccgcc agtatctcct ccacaccgac atgagcgcca
     3181 accacgacgg ggcgggcacg gtgcagccgg gggagtacac cagcacctgg ttgacctggg
     3241 tttcgaactc gcgcacgtat gagaacccga tgcagtgcga tgtgtgggac cgcacgatgt
     3301 acgagttggt gacgctggcc gaggccaacg tcggcgccgt ctccggctca ccaacgccgt
     3361 acgccacggt gacgggcgcc aacaccaacg cggcactgtt gacgttcgag ttcgcctcca
     3421 tcccgatcga ctacgacacg gatggcgctg gccctgatac gggtgacgac ccgctgggtg
     3481 gtggattcaa ccctggcacc ggtcgctacg gcggcacctg gtcgagccag gccgcagccg
     3541 tcgcggccgg ttgcatgccc ggcgcggagg acgtcacctg ggtgaccgat cccaccacct
     3601 tgccgggcgg cgcggcctcg gccaacgccg tgcgcgtgtc cggtcctgnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnncc gacgatgggc ccgggcagcg accgccttct ccgcgtcggt
     3721 ttcaaggtgc gcaacacctt cgacggtggc ccgcacaacg gccaagccat tccggtcggt
     3781 gccatcggcg ccaactacgg caacgtctcg ggcgtcagcg gcaccttccc cgccaacggg
     3841 tacaaccccg tcgtcgacgc cggcctcagc ggtgaccgtc tcacctggac ccgcgcggtg
     3901 ctcggtgtca agaagctgac cgtcgccgtc gacggcatgt gcgacgcacc gtgcgcgcag
     3961 ccgatcgacg tcaacggctc ggtgctcgcg ggcaagccga tcatttggga gattcgtccg
     4021 gtggtcaatg ccgggagccc cacgccggca ccggtcaaca acctgacgct caccgacgtc
     4081 ttgccggtgt acgtcgaata cgacgacgct tgcaccgcgg ccctcaccgg cggcacgccc
     4141 gccgacgacg tgttgttcaa cacgcccgct gccggccaga cgacgctggt gtggaacgtc
     4201 ggcacggtga tccccaacac ggagatcacg ccctatcgga tctgtactca gaccgacccg
     4261 atcgcccccg cccc
//
LOCUS       DAORNF010000231         4255 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_15478_length_4255_cov_1.667143, whole genome shotgun sequence.
ACCESSION   DAORNF010000231 DAORNF010000000
VERSION     DAORNF010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4255
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15478_length_4255_cov_1.667143"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..634
                     /locus_tag="PLV68_10060"
     CDS             <1..634
                     /locus_tag="PLV68_10060"
                     /inference="COORDINATES: protein motif:HMM:NF024122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HRE01634.1"
                     /translation="QAPGLSAMDRKDRGDFLRYFYRRYEDAPVDQIDEDSREMLTQLI
                     VTKSFPAGIRRVREHKAMGHRTVLITGAMDFAVSGLKPLFDEIVAAEMTVRPDGTYSG
                     ELAKVPPTGETRAQVLADYCAAEGLRLDESIAYADSTSDLPLLECVGFPVAVNPETRL
                     AAIARKRGWLVENWSKAPGGPRPLLPVGPLLAERERNRRFRGVVLKAGRS"
     gene            648..1856
                     /locus_tag="PLV68_10065"
     CDS             648..1856
                     /locus_tag="PLV68_10065"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HRE01635.1"
                     /translation="MKALQVSRSVPRLGLARIASAISPLTAAKLGPLKLISTDPPGSL
                     GDGWHTVATRLSGICGSDLSTVEGHASIYFDDLVSFPFTPGHEVVGQLADGTRVVIEP
                     VLGHEARGCQPPFPGAAPGDGDDYAHLVLGPLEPGIQTGFCHSTGGGWATFFQAHESQ
                     LHRVPDDMSDETAVMVEPLAGGIHTALMCAPLVAGIDKPVVAVLGAGTMGLCAIAGLR
                     RFLPDVHVIAAARYPHQQAYARALGAQQVVAPKELARAVRSAVGCHVIGDYLSSGAHV
                     TIDAVGNSGSIESCLHVTRPRGTVVLMGMPAEVTVDLTGLWHRETRLAGSYTYGTERL
                     PDGTPHGSTARTFDLAIELAAALDLGRLVSATYPLDRYEDAIAHASSAGRRGAVKIAF
                     DLRDAPKEPR"
     gene            1856..>3306
                     /locus_tag="PLV68_10070"
     CDS             1856..>3306
                     /locus_tag="PLV68_10070"
                     /inference="COORDINATES: protein motif:HMM:NF021387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HRE01636.1"
                     /translation="MPRPGFVLEVDRNTPPVLFWRGENFSLEKLPADRSRVIYAPEPL
                     PPLDDIDGAIVHALNNPIDHDPLPALLFPGMKLTIAFDDVSLPLPPMQRPDVRQRVIE
                     AVLDMAADAGVDDVHIIAALALHRRMTEAELRHAVGDRVYDAFAPRGMLYQHDAEDPD
                     QLEYLGQTPHGEDVEINKRAATSDLLVYVNINLVAMDGGWKSTATGLSSYKSLQHHHN
                     PHTMEHTRSFMDRHKSELHKSNWRMGKVLLDAGVKVFQIETTLNTDTFPKPFAFLSKR
                     EWEWSVRDRASFVGTAAALKRTPARLARNIFHSIEAPHQMTSIQAGEVEAVHEVTTEN
                     VWKQQLVEVRTGVCAPPRAPGAGAGPDRHPDDGHPVHLPLQRQLDHEPDPGDVYGSRV
                     PVQHVPRQAAGARRRRGDHDPPDAVGLPPGASSELHRLLRAGAGRDHRPPCDAPQVRE
                     TLRRGRVVQAPLSHQLRLPRRAPVLHVVLGCTR"
     assembly_gap    3307..3391
                     /estimated_length=85
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3486..>3711
                     /locus_tag="PLV68_10075"
     CDS             3486..>3711
                     /locus_tag="PLV68_10075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01637.1"
                     /translation="MSSEFAMARLIHRDDAPRSVTSLRRAARMAGKVETAIAKAAKPL
                     ATKGFPYRKPNVPRTAVLPKKKSTLGADFET"
     assembly_gap    3712..3736
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3737..>4255
                     /locus_tag="PLV68_10080"
     CDS             <3737..>4255
                     /locus_tag="PLV68_10080"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRE01638.1"
                     /translation="RLARALWVEGPMRALIGAVATPTVKGTDRLEDLRRADDPPPLIF
                     TPNHHSHLDTPLAISVIPEPWRHKLIVAAAADYFFTSRVKGSLSALTLNALPIDRDAT
                     GRKSSDQIKALMAEGWSLLIYPEGGRSPDGWGQDFKGGAAYLAGRTGAAVVPMFIDGT
                     GPIFGKGMKRPKP"
BASE COUNT          713 a         1510 c         1309 g          613 t
ORIGIN      
        1 ccaagcaccg ggcctgtcgg cgatggaccg caaggatcgg ggcgacttcc tgcgctactt
       61 ctatcgccgc tacgaggacg cgccggtcga ccagatcgac gaggacagcc gcgagatgct
      121 gacccaactc atcgtcacca agagctttcc cgcaggcatt cgccgggtgc gtgagcacaa
      181 ggcgatgggc catcgcacgg tgctgatcac cggcgcgatg gacttcgccg tgtccggcct
      241 gaaacccctg ttcgacgaga tcgttgcggc cgagatgacc gtgcgtcccg acggcacgta
      301 cagcggcgaa ctggccaaag tgccgcccac cggcgagacg cgggcccagg tgctggccga
      361 ctactgcgcg gccgaagggc tgcgcctcga cgagtcgatc gcctacgccg acagcaccag
      421 cgacctgccg ctgttggagt gcgtcgggtt tcccgtcgcg gtcaaccccg agacccgcct
      481 ggccgcgatc gcccgcaagc gcggctggct cgtcgaaaac tggtcgaagg ccccgggagg
      541 accacggccg ctgctgcccg tcggcccgct gttggccgag cgcgaacgca accgccgctt
      601 ccgcggcgtg gtcctgaaag ctggccgctc atgaaagagg tgcggtcgtg aaggcgctcc
      661 aggtctcgcg cagcgtcccc cgcctggggc tggcgcgcat cgcctcggcg atcagcccgc
      721 tcaccgcggc caagctcggc ccgctcaagc tgatctccac cgacccaccc gggtcgttgg
      781 gcgacggctg gcacaccgtc gccacccgcc tgtccggcat ctgcggcagc gacctgtcca
      841 cggtcgaggg tcacgcgtcg atctacttcg acgatctggt cagcttcccg ttcacccccg
      901 gccacgaggt ggtcggccaa ctcgccgacg gcacgcgcgt ggtgatcgaa ccggtgctcg
      961 gccacgaggc ccgcggctgc caaccgccat tccctggcgc cgcgcccggc gacggcgacg
     1021 actacgccca tctcgtgctc ggtccgctcg aaccgggcat ccaaaccggg ttctgccact
     1081 cgaccggtgg tggctgggcg acgtttttcc aggcccacga gtcgcagttg caccgcgtgc
     1141 ccgacgacat gagcgacgag accgcggtga tggtcgagcc gctcgccggc ggcatccaca
     1201 ccgcgctgat gtgcgcgcca ctggtcgccg ggatcgacaa gcccgtggtc gccgtgttgg
     1261 gagcgggcac gatggggctg tgcgcgatcg ccggcctgcg ccggttcctg cccgacgtgc
     1321 acgtcatcgc tgcggcccgc tatccccacc agcaggccta cgcccgtgcc ctcggcgcgc
     1381 agcaggtggt agcccccaag gagctcgcca gggccgtgcg cagtgccgtc ggctgccacg
     1441 tcatcggcga ctatctcagc agtggtgccc acgtcaccat cgacgcggtc ggtaacagcg
     1501 gctcgatcga gtcgtgcctg cacgtcaccc gcccccgcgg cacggtggtg ctgatgggca
     1561 tgcccgccga ggtcaccgtc gacctcaccg ggttgtggca ccgcgaaacc cgcctggccg
     1621 gttcgtacac ctacggcacc gagcgcctgc ccgacggcac cccacatggc agtacggcgc
     1681 gcacgttcga tctcgcgatc gaactcgccg ccgccctcga tctcggccga ctggtgtcgg
     1741 ccacctatcc cctcgaccga tacgaagacg cgatcgccca tgcctccagc gccgggcgcc
     1801 gcggtgcggt gaagatcgca ttcgaccttc gtgatgcgcc caaggagccc cgctgatgcc
     1861 ccgtcccgga ttcgtcctcg aggtcgaccg caacaccccg cccgtgctgt tctggcgcgg
     1921 cgagaacttc agcctggaga agctgcccgc cgaccgcagc cgggtgatct acgcgcccga
     1981 gccgctgccg ccgctcgacg acatcgacgg cgcgatcgtc cacgcgctga acaacccgat
     2041 cgaccacgac ccgctgcccg cgctgctgtt ccccggcatg aagctgacga tcgcgttcga
     2101 cgacgtcagc ctgcccttgc caccgatgca gcgccccgac gttcgccagc gggtgatcga
     2161 agcagtgctc gacatggccg ccgatgcggg tgtcgacgac gtgcacatca tcgccgcgtt
     2221 ggcgttgcac cggcgaatga ccgaggccga actgcgccac gccgtcggtg accgggtcta
     2281 cgacgcgttc gcgccgcggg gcatgctcta ccagcacgac gccgaagacc cggaccaact
     2341 cgaatacctg ggccagaccc cgcacggcga ggacgtcgag atcaacaagc gggccgccac
     2401 gagcgacctg ctggtgtacg tcaacatcaa cctggtcgcg atggacggcg ggtggaagag
     2461 caccgccacc gggctgagca gctacaagag cctgcagcac caccacaacc cgcacaccat
     2521 ggagcacacc cgcagcttca tggaccgcca caagagcgaa ctgcacaaga gcaactggcg
     2581 gatgggcaag gtgttactcg acgcgggcgt caaggtattc cagatcgaga ccaccctcaa
     2641 caccgacacg ttccccaaac cgtttgcgtt cttgtccaag cgcgagtggg agtggagcgt
     2701 gcgtgaccgg gccagcttcg tgggcaccgc cgccgcgctc aagcgcaccc cggcccgcct
     2761 ggcccgcaac atcttccaca gcatcgaagc gccgcatcag atgaccagca tccaagcggg
     2821 cgaggtcgaa gcggtgcacg aggtgaccac tgagaacgtg tggaagcagc aactggtcga
     2881 ggtgcgcacg ggtgtatgtg cgccaccccg agcacctggt gccggtgcag ggccagaccg
     2941 acatcctgac gatgggcatc ccgttcatct gcccctacaa cgtcaactcg atcatgaacc
     3001 cgatcctggt gatgtgtatg ggtctcgggt acctgttcaa catgtaccgc ggcaagccgc
     3061 tggtgcgcga aggcggcgtg gtgatcatga cccacccgac gccgtgggac ttccacccgg
     3121 tgcatcatcc gagctacatc gacttcttcg agcaggtgct ggccgagacc accgaccccc
     3181 atgtgatgca ccacaagtac gagaaacgct tcgccgagga cgagtggtac aagcacctct
     3241 atcgcaccag ctacgcctac cacggcgtgc acccgttcta catgtggtac tggggtgcac
     3301 acggcannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngcgttcgag atcgcgtccg acgtcgtcgg
     3421 ccgctctccc acgatcaccc acctccacac gccgccgatc ctggtggccg acgtcaagtg
     3481 aacgggtgtc gagtgagttc gccatggccc gcctgatcca ccgcgacgac gcaccacgat
     3541 cggtcacctc gctgcgccgc gcggcgcgca tggccggcaa ggtcgaaacc gccatcgcca
     3601 aggcagccaa gccgctggcg accaaggggt tcccgtaccg caagcccaac gtgccgcgca
     3661 ccgcagtgct gcccaagaag aagtccaccc tcggggccga cttcgagacc gnnnnnnnnn
     3721 nnnnnnnnnn nnnnnncgtc tcgcccgcgc gctctgggtc gaaggcccga tgcgggcgct
     3781 gatcggtgcg gtcgcgaccc cgaccgtgaa gggcaccgac cggctcgaag acctgcgccg
     3841 cgccgacgat cctccgcccc tgatcttcac gcccaaccac cacagccacc tcgacacccc
     3901 actggcgatc tcggtcatcc ccgagccatg gcggcacaag ttgatcgtcg ctgcggccgc
     3961 cgactacttc ttcaccagtc gggtcaaggg ttcgctgtcg gcgctgaccc tcaacgcgtt
     4021 gccgatcgac cgcgatgcca ccggacgcaa gtcgtccgac cagatcaagg cgctgatggc
     4081 cgaggggtgg agcctgctga tctatcccga aggcgggcgc tcacccgatg gctgggggca
     4141 ggacttcaag ggcggtgctg cgtacctggc cggacgcacc ggcgcggcgg tggtgccgat
     4201 gttcatcgac ggaaccgggc cgatcttcgg taagggcatg aaacgcccca agcct
//
LOCUS       DAORNF010000232         4243 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_15539_length_4243_cov_1.979465, whole genome shotgun sequence.
ACCESSION   DAORNF010000232 DAORNF010000000
VERSION     DAORNF010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4243
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15539_length_4243_cov_1.979465"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /locus_tag="PLV68_10085"
     CDS             complement(<1..594)
                     /locus_tag="PLV68_10085"
                     /inference="COORDINATES: protein motif:HMM:NF036808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01639.1"
                     /translation="MAVLWRAMGDDGVLVLRAGRDKSVRRRHPWVLSGSVERVEGDPA
                     PGATVRVVSADGEMLGRAAYSPASRLRARMWTFDDATTVDAELIAARVTTAAARRARL
                     LHDTDTDAVRLVFSEADDLAGVIADRYGSTVVLQLGSAGADRWRGEITDALAALPGVD
                     SVFERSDLDARQREGLTARTGLLAGPMPTDELIIHEGP"
     gene            655..897
                     /locus_tag="PLV68_10090"
     CDS             655..897
                     /locus_tag="PLV68_10090"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HRE01640.1"
                     /translation="MAMSLVSARTFNQSPSKVKALAKEGPVFVTEHGRPTAVVLTIDD
                     YERLRGVGNVRESLRMDVDVDFEPLVLRDLGRVADL"
     gene            894..1307
                     /locus_tag="PLV68_10095"
     CDS             894..1307
                     /locus_tag="PLV68_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRE01641.1"
                     /translation="MSFLLDTNVVSELRKPQADARVVSWASGLSMRDAFLSVVTVREI
                     EAGVLSMERRDPSQGARLRSWFLESVLGAYADRILSVDLAVARRTATLHVPNPQPERD
                     SLIAGTALVHGLTIATRNLSDFQAMAVDVFNPWSD"
     gene            complement(1337..1840)
                     /locus_tag="PLV68_10100"
     CDS             complement(1337..1840)
                     /locus_tag="PLV68_10100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01642.1"
                     /translation="MRRKTDMWNFEDGGDTAVATAERDELIAVDSAYTDVRLEVLEQQ
                     VRTQFVAIATNHEAAVQSVETARAEARADLDREKATLIGLLERVRDECHGTAPAHTVA
                     APVAATGPDPEALARIDALEDRFTELSRQMAVLLRSQQELANSITFMFERQLHSAGFA
                     ESCAPTP"
     gene            2053..>2396
                     /locus_tag="PLV68_10105"
     CDS             2053..>2396
                     /locus_tag="PLV68_10105"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRE01643.1"
                     /translation="METPIEDIETPSKRDKGFTLVELLIVIVILGILATVTVFAVRGI
                     TDKGTKSACQADKKTLEVAIESYFAQYGGTAIPTGTDPLPANPAPNFTAVGADAENTL
                     KNAGYLRDVSPK"
     assembly_gap    2397..2474
                     /estimated_length=78
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2751..>3127)
                     /locus_tag="PLV68_10110"
     CDS             complement(2751..>3127)
                     /locus_tag="PLV68_10110"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HRE01644.1"
                     /translation="CMGGRVAFQAALEHGIGAAVSFYGGGIAAPHPVGWSALGGRAAE
                     LRAPVLAIFGDADHTIAVDDVEAWRPVLATAPVATDIVRYAEAGHGFHCDARGAYHAE
                     AARDAWARTQAWLDRFVPATPA"
     assembly_gap    3128..3141
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3142..3487)
                     /locus_tag="PLV68_10115"
     CDS             complement(<3142..3487)
                     /locus_tag="PLV68_10115"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HRE01645.1"
                     /translation="MSTVASTTVEFEGLPLAVADPASEPGHSAVDRAVIVIQEAFGVN
                     DHIEDVGRRFAALGWVAVAPHLYHRDGSPTFAYDDLAGARPHMAALTDEGVGADLATA
                     LAWLASRGITTER"
     gene            complement(3484..>4243)
                     /locus_tag="PLV68_10120"
     CDS             complement(3484..>4243)
                     /locus_tag="PLV68_10120"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HRE01646.1"
                     /translation="AGRCPVIPGVVATATHDAVAQAAQYEQLGVAGVVAIRQPGLPTS
                     PEGVESFYRDIAAAVSVPVVLYTNPGLLGVDLSIATIRRLADVANITAIKDASGNTGR
                     ILSVLTRTDLDVFSASAHIPAVVMRLGGVGWMGGPASAVPAASVLLYDLCRAGQWNDA
                     FALQRVLWPLHEVFLEHSLAAVVKHAVGRRGIDVGGPIAPQTSLPAEVGRAIDAALAT
                     IDEFVDRFTVAAPPSPPPPSARPTRPARPTRSIS"
BASE COUNT          688 a         1378 c         1400 g          685 t
ORIGIN      
        1 cgggccttcg tggatgatca gctcgtcggt gggcatcggc ccggcgagca ggccggtgcg
       61 cgcggtcagc ccttcccgtt ggcgggcgtc gaggtcggaa cgctcgaaca cgctgtcgac
      121 gccgggcaac gccgcaagtg cgtcggtgat ctctccccgc cagcgatcgg ccccggcgct
      181 gccgagctgc agcaccacgg tcgagccgta gcggtcggcg atcacgcccg ccaggtcgtc
      241 ggcctcgctg aacaccagcc gcacggcgtc ggtgtcggtg tcgtgcagca ggcgtgctcg
      301 ccgcgcggcc gcggtggtga cgcgtgccgc gatcagctcc gcatcgacgg ttgtggcgtc
      361 gtcaaaggtc cacatccgcg cccgtaaccg cgacgcgggc gagtacgcgg cccgccccag
      421 catctcgccg tccgcgctga cgacgcgcac ggtcgccccc ggagccgggt caccttctac
      481 ccgttcgacc gacccgctca gcacccacgg atgccgccgc cgcaccgact tgtcccgccc
      541 tgcccgcagc accaacacac cgtcgtcacc catcgcccgc cagagtacgg ccatccagca
      601 gatgccggaa cgaggcacaa atgtagttac gtagtatcat ttcatgagga ggtgatggca
      661 atgagtttgg tcagcgcgcg gacgttcaat cagtcgccgt ccaaggtgaa ggccttggcc
      721 aaggagggac ctgtattcgt gaccgagcat ggtcgaccaa ctgcggtcgt cctcacgatc
      781 gacgactacg agcgtctgcg gggtgtcggc aatgtgcggg agtcgttgag gatggatgtc
      841 gatgtcgact tcgaacccct cgtcctccgt gatcttggcc gggtagccga tctgtgagct
      901 tccttctcga taccaatgtc gtcagtgaac ttcgtaaacc tcaggccgat gcgagagttg
      961 tctcctgggc aagcggattg tcgatgcgtg acgcattcct cagcgtcgtg accgttcgtg
     1021 agatcgaagc gggcgtgttg tccatggagc gccgcgatcc atctcaaggt gcccggctac
     1081 gctcgtggtt tctcgaaagc gtgctcggcg catacgctga ccgcattctg tcggtcgacc
     1141 tcgctgtagc gcggcggacg gccaccctgc acgtgccgaa cccgcagcct gagcgcgatt
     1201 cgctgatcgc cggaacagcc ctcgtccatg gcttgaccat tgccactcgc aatctcagcg
     1261 acttccaggc aatggcggtc gacgtgttca atccgtggag cgattgaccg ccgagcgacc
     1321 accgagtggc tgattgtcag ggggtggggg cgcaggactc ggcgaagccg gcggagtgga
     1381 gttggcgctc gaacatgaag gtgatcgagt tggccagttc ttgttggcta cgcagcagca
     1441 cggccatctg acggctgagc tcggtgaagc ggtcctccag cgcgtcgatg cgggccagcg
     1501 cttcggggtc ggggccggtc gcggcgacag gcgcggcaac ggtgtgggcg ggagcggtgc
     1561 cgtggcactc gtcgcggact cgctcgagca ggccgatcaa cgtcgccttc tcgcggtcga
     1621 ggtcggctcg ggcctctgcc ctggcggtct ccaccgactg caccgctgcc tcgtgattcg
     1681 ttgcaattgc aacgaactga gtcctcacct gttgttccaa cacttccagg cgaacgtcgg
     1741 tgtaggccga gtcgaccgcg atcaactcgt cgcgctccgc ggtcgcaacc gcggtatcac
     1801 cgccgtcttc gaagttccac atgtcggtct tgcgtcgcat cggtctcact atcggcccgc
     1861 ggcggcgctg gcttgagcgt tcgcgtccgt acagaggtgt gtcatgggcc gcccaaggcg
     1921 acgtgaggat ttctccaaga aagttctcaa gcttgcagta tgggtgccga aacctctctc
     1981 gcaccgagac acgggtcacc cttgagtcgg tgtgtttcgt gggaaatcac tagcggagga
     2041 aacgcaccca tcatggaaac cccaatcgaa gacattgaaa cccccagcaa gcgcgacaag
     2101 ggcttcaccc tcgtcgagtt gctgatcgtc atcgtcatcc tcggcatcct cgccaccgtg
     2161 accgtgttcg ccgtccgcgg catcacggac aagggcacga agagcgcctg ccaggctgac
     2221 aagaagaccc tcgaagtcgc catcgagtcg tacttcgctc agtacggtgg tacggcgatc
     2281 ccaacgggta ccgatccgtt gcccgcgaac ccggccccga acttcacggc cgttggcgcg
     2341 gacgctgaga acaccttgaa gaacgctggt tacctgcgcg acgtctcgcc caagtgnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnaacgag tgggaggtgg gctttggccc gcctcccact cggcgttttc
     2521 gggcgcggtc gtggccaatg ccaatcccca actcaccgga gcggggcgat atcgcacacg
     2581 cccggtgaag cgcgaaacga gatccgattt gcactcgttc gtgggttcga cgacgtccga
     2641 agaaagagag aaaacgagaa gaagcggctg atgttgggct caggatcccc aagctctgtc
     2701 gcgccagccg gcattgcgcc aggccggcat cgcgtcaggc cggcatcgcg tcaggcgggc
     2761 gtggcgggca cgaagcgatc gagccaggct tgggtgcgcg cccacgcatc gcgagcggcc
     2821 tcggcgtgat aggcgccacg tgcgtcacag tggaaaccgt gcccggcctc cgcgtagcgc
     2881 acgatgtcgg tcgccaccgg agccgtcgcc agcaccggcc gccaggcctc cacgtcgtcg
     2941 acggcgatgg tgtgatcggc gtcgccgaag atcgccagga cgggggcgcg cagttctgcc
     3001 gcccgcccgc cgagggcgct ccagccaacg ggatggggcg cggcgatgcc acctccgtag
     3061 aacgacaccg ctgcaccgat gccgtgctcg agcgccgctt ggaaggcgac tcgtccgccc
     3121 atgcagannn nnnnnnnnnn ntgcgctcgg tggtgatgcc gcggctcgcc agccacgcca
     3181 gcgcggtggc gagatcggca ccgaccccct cgtcggtcag cgcggccatg tgagggcgag
     3241 cgcctgcgag atcgtcgtag gcgaaggtcg gcgagccgtc acggtgatac agatgcggcg
     3301 cgacggcgac ccagcccaac gctgcgaagc gacggccgac gtcttcgatg tggtcgttga
     3361 ccccgaaggc ttcctggatg acgatcaccg cccggtcaac cgcgctgtgc ccgggttcgc
     3421 tcgcaggatc ggcgaccgcc agtggcagtc cctcgaactc gacagtggtt gatgcgacgg
     3481 tgctcatgag atgctcctgg ttggtcgggc gggcctcgtc ggccgcgcgg acggtggggg
     3541 tggcgacggc ggggccgcca cggtgaagcg atcgacgaac tcgtcgatcg tggcgagggc
     3601 agcgtcgatg gcgcgcccga cctctgccgg cagcgacgtc tggggcgcta ttggaccacc
     3661 gacgtcgatg ccgcggcggc caaccgcgtg tttgacgacc gccgcgagcg agtgttcgag
     3721 gaacacctcg tgcaacggcc agagcacgcg ctgcaacgcg aaggcgtcgt tccactgccc
     3781 cgctcggcac aggtcgtaca acaacacgct ggcggcgggg accgcgctgg ccggtccgcc
     3841 catccatccc accccgccga gacgcatcac cacggccggg atgtgcgccg acgcgctgaa
     3901 cacgtcgagg tcggtgcgcg tcagcaccga caggatccga ccggtgttgc ccgacgcatc
     3961 cttgatggcg gtgatgttcg ccacgtcggc caaccggcgg atcgtggcga tcgagaggtc
     4021 gaccccgagc aggccggggt tggtgtacaa caccaccggc acgctgaccg cggccgcgat
     4081 gtcgcggtag aacgactcga cgccctccgg tgaggtggga agcccaggct ggcggatcgc
     4141 gacgacaccg gcgacgccca gttgctcgta ctgcgcggct tgggcgacgg cgtcgtgcgt
     4201 ggcggtcgcg accacgcccg ggatcactgg gcagcgcccg gcc
//
LOCUS       DAORNF010000233         4233 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_15589_length_4233_cov_1.819292, whole genome shotgun sequence.
ACCESSION   DAORNF010000233 DAORNF010000000
VERSION     DAORNF010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4233
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15589_length_4233_cov_1.819292"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="PLV68_10125"
     CDS             complement(<1..241)
                     /locus_tag="PLV68_10125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01647.1"
                     /translation="MRRSLAGVLFGFASMFASLAVSGFWLQFTAFSPSHTRAAAKTVL
                     GDNKIKNELAKLIADSTVVALNGIDAERYPTIDPEP"
     assembly_gap    287..296
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            562..>952
                     /locus_tag="PLV68_10130"
     CDS             562..>952
                     /locus_tag="PLV68_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HRE01648.1"
                     /translation="MALEREKALEMALATIDKQFGKGSIMRMGEKTSMGIETVPTGAL
                     SLDLALGVGGLPRGRVVEIYGPESSGKSTLAMHVVAEAQRNGGICAYVDAEHAMDPVY
                     AKSIGVDIDQLLISQPDTGEQALEIADM"
     assembly_gap    953..1015
                     /estimated_length=63
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1016..>1330
                     /locus_tag="PLV68_10135"
     CDS             <1016..>1330
                     /locus_tag="PLV68_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017955955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA recombination/repair protein RecA"
                     /protein_id="HRE01649.1"
                     /translation="AEIEGEMGDSHVGLQARLMSQALRKLTGSLSKTNTIAIFINQLR
                     EKIGVMFGSPETTPGGRALKFYSSVRLDIRRIESIKDGAEVVGSRTRVKVVKNKVAPP
                     FR"
     assembly_gap    1331..1438
                     /estimated_length=108
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1723..3246
                     /locus_tag="PLV68_10140"
     CDS             1723..3246
                     /locus_tag="PLV68_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HRE01650.1"
                     /translation="MNVVVILLDSLNRHGLGCYGGTEWETPNLDRFAQRAVRFTNHHT
                     GSLPCIPARHDLLVGAWDFLWKPWGSIELWEEAITASMRRAGVVTQLITDHPHLFETG
                     GENYHTDFTAWYYLRGHESDAWKTRPDASWIGAPNFGRGHTHYDNSRGYFTDEDDFPG
                     PRTMAAAAHWLGHHAPHHRQAGQRFFLWVDEFDPHEPFDTPEPWASMYDPDWSGPNLI
                     WPPYKVGALADGTLDAREARQIRAQYGGKLAMIDHWLGRVLDQLDATGGWDDTAVVVC
                     TDHGHYLGEPDVHGKDVWGKPSVPVMRTLGHTPLLVWWPGSSLGGGTCDALTTTADIH
                     ATLLDAFGITAAHRTHGTSLLPLLRGEATSVRDWVLTGVWGREVHLITNQWRYARGPQ
                     GPNTPLSMWSNRWSSMPIPSLPDLRIPPPDDRACLDRMPGSTIPVIRQPFVPGDMLPF
                     WAYAPQYATLLFDRIEDPDETVDRSADRIAADAAELLRVALTEVAAPDDQFVRLALT"
     gene            complement(3281..>3842)
                     /locus_tag="PLV68_10145"
     CDS             complement(3281..>3842)
                     /locus_tag="PLV68_10145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01651.1"
                     /translation="QLLDVMTGTFVPDGISSQPDAFNSECCGGYDYLRVEHAGSLAWS
                     VSAELITVWFPSDSPGPGITVDLFGTNDSVLITPTLGWDAIQATCGTDPVCFPVRVAI
                     GGLSPGDAVEVACWVLAGDEWDRRGTPTAVAVSPDGSIYLASACVVGAHEPVVVTVDG
                     AASAPLVLDGEPAPTVPFPATTTTSG"
     assembly_gap    3843..3989
                     /estimated_length=147
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3990..4187)
                     /locus_tag="PLV68_10150"
     CDS             complement(<3990..4187)
                     /locus_tag="PLV68_10150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01652.1"
                     /translation="MNFDWLGANYVLRRVDANGVERWRRSDLTPYGGEGFRTSNSQQR
                     DGRDVTLVYACLGTPAGPQDGG"
BASE COUNT          677 a         1336 c         1271 g          621 t
ORIGIN      
        1 cgggttcggg atcgatggtg gggtagcgct cggcgtcgat gccgttgagc gcgaccacgg
       61 tcgagtcggc gatcagcttg gccaactcgt ttttgatctt gttgtcgccg agcacggtct
      121 tggctgcggc acgggtgtgg ctgggagaga aagcggtgaa ctgcaaccag aagccgctca
      181 cggccaggct ggcgaacatc gaagcgaatc cgaacaagac accagcaagg gagcgacgca
      241 cggtcggaat cctggcactg cgcgcttgtt cggtgtggtc agcaacnnnn nnnnnngtgg
      301 tcgcgtagtt gtcgcgtagt tgtcacgtac gtcgtgacct gggtacgcaa ccccgccagc
      361 ggtccggact gcgatcctgc ccgaattgct tgcacgaacg tctgtacgaa gttacgctgc
      421 tgtcgttcgc cgccggagcc ggacacagcg ctcggacaac gcactgtgtc acaccccttc
      481 cgtacggtgc cacccatcag atcggccgct gcaatccagc ccggcccgcc agccaatcac
      541 acagcaggag acagaaatac catggcactc gaacgagaga aggcgctcga aatggcgctg
      601 gcgaccatcg acaagcagtt cggcaagggg tcgatcatgc ggatgggcga aaagacctcg
      661 atgggcatcg aaaccgtgcc cacgggcgca ctcagcctcg acttggccct tggcgtcggc
      721 ggtctaccgc gcggtcgtgt ggtcgagatc tacggcccgg agtcctcggg taagtccacc
      781 ttggcaatgc acgtcgtcgc cgaagcccaa cgcaacggtg gcatctgcgc ctacgtcgac
      841 gccgagcacg cgatggaccc ggtctacgcc aagtcgatcg gcgtcgacat cgaccagctg
      901 ctcatctcgc agcccgacac cggtgagcag gcgctcgaga tcgccgacat gcnnnnnnnn
      961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngggcc
     1021 gagatcgaag gcgagatggg cgacagccac gtcggcttgc aggcccgcct catgagccag
     1081 gccctgcgca agctcacggg cagcctcagc aagaccaaca cgatcgcgat cttcatcaac
     1141 cagttgcgtg agaagattgg cgtcatgttc ggctcgcccg agaccacacc cggtggccgt
     1201 gcgctcaagt tctactcgtc ggtgcgtctc gatatccgcc gcatcgagag catcaaggac
     1261 ggcgccgaag tcgtcgggtc gcgcacccgg gtcaaggtgg tcaagaacaa ggtcgcgcca
     1321 ccgttccgcc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncg
     1441 agcagttggg ccagggtcgc gagaacgcca agaactacct gcacaacaat cccgaggtca
     1501 tggtcgagat ctccgagcgg gtgcgccagc aggccggtct cacgcccgac ctcgacacgt
     1561 tcggcaccaa ggacgacgag ccgatctcgc tcgaatcctg agctcttcgg ggcgtgcgcc
     1621 ccggtgcctc acgccgctgt ggtcggccgc actctcgcgt agggtgcggc cagcagccca
     1681 gccgttcgta ctcgatcggg cggccgacca tcagggggag acgtgaacgt cgtcgtgatc
     1741 ttgctcgaca gcctcaaccg tcacgggttg gggtgctacg gcggtacgga atgggagacc
     1801 cccaacctcg accgcttcgc gcagcgggcg gtgcggttca ccaaccacca cacgggttcg
     1861 ttgccgtgca tcccggcccg ccacgatctg ctggtcggcg cgtgggactt cctgtggaag
     1921 ccgtggggct cgatcgagct gtgggaagag gcgatcaccg cctcgatgcg ccgggccggt
     1981 gtggtcaccc agctgatcac cgatcatccc catctgttcg agaccggcgg cgagaactac
     2041 cacaccgact tcacggcgtg gtactatctg cgcggtcacg agtccgacgc gtggaagacc
     2101 cggcccgatg cgtcgtggat cggcgcgccc aacttcggcc gcggccacac ccactacgac
     2161 aactcccgtg gctacttcac cgacgaggac gacttccccg gcccgcgcac gatggccgcc
     2221 gcggcgcact ggttgggtca ccacgcgccc catcaccgcc aggcgggaca gcggttcttc
     2281 ttgtgggtcg acgagttcga cccacacgag ccgttcgaca cacccgagcc ttgggcgtcg
     2341 atgtacgacc ccgactggag cggtccgaac ctgatctggc ctccgtacaa ggtgggcgct
     2401 ctggccgacg gcacgctcga tgcccgcgag gcgcgccaga tccgggcgca gtacggcggc
     2461 aagctggcga tgatcgacca ctggctgggc cgggtgctcg accaactcga cgcgaccggc
     2521 ggctgggacg acacggcggt cgtggtctgt accgatcacg gtcactatct cggagagccc
     2581 gacgtgcacg gcaaggacgt gtggggcaag ccgtcggtac cggtgatgcg caccctgggc
     2641 cacacgccgc tgttggtctg gtggcccggc tcgtcgcttg gcggcggtac ctgtgatgcc
     2701 ctgacgacca cggccgacat tcacgcgacg ttgctcgacg cgttcggcat cactgccgcc
     2761 catcgcaccc acggcacgtc gctgctgccg ctgctgcggg gcgaggccac ctcggtgcgc
     2821 gactgggtgc tcactggggt gtggggtcgt gaggtgcact tgatcaccaa ccagtggcgg
     2881 tatgcccgcg ggccgcaagg gccgaacacg cccttgtcga tgtggtcgaa ccggtggagc
     2941 tcgatgccga tccccagcct gcccgacttg cggatcccgc cacccgacga ccgggcgtgc
     3001 ctcgaccgca tgcccggctc caccatcccg gtgatccgcc agccgttcgt ccccggcgac
     3061 atgctgccgt tctgggccta cgccccgcag tacgcgacgc tgctgttcga ccgcatcgaa
     3121 gaccccgacg agacggtcga ccgctccgcc gaccgcatcg ccgccgacgc cgccgaactg
     3181 ctccgcgtcg ccctcaccga ggtcgcggcc cccgacgacc agttcgtccg tctcgctctt
     3241 acctgactcg ccttgacctg actcgccttg acctgcctcg tcagccggac gtggttgtcg
     3301 ttgcgggaaa gggaactgtc ggggcgggtt cgccgtcgag cacgagcggt gctgaggcag
     3361 cgccgtcgac ggtgacgacg accggctcat gcgcgcccac cacacacgcg ctcgcgagat
     3421 agatcgaacc gtcaggcgac acggcgaccg cagtcggtgt accccgacgg tcccactcgt
     3481 cgccagcgag gacccagcaa gcaacctcaa cggcgtctcc gggtgacagg ccgccgatgg
     3541 cgacgcgcac cgggaagcaa acgggatcgg tgccgcacgt ggcctggatg gcgtcccagc
     3601 ccagtgtcgg tgtgatcagc accgagtcgt tcgtcccgaa caagtcgacc gtgataccgg
     3661 gaccgggcga gtcgctcgga aaccagaccg tgatgagttc ggcggacacc gaccaggcga
     3721 gcgacccagc gtgctcgacc cggagatagt cgtaacctcc gcaacactcc gagttgaaag
     3781 cgtcgggctg cgacgagatg ccatccggca cgaacgtgcc ggtcatcaca tcgagcagtt
     3841 gannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnng ccgccgtctt gtggcccggc gggcgtaccg
     4021 agacaggcgt agaccagcgt gacgtcgcgt ccgtcgcgct gctggctgtt gctggtacga
     4081 aagccctcgc cgccgtacgg cgtgaggtca ctgcgacgcc accgctcgac accgttggcg
     4141 tcgactcgcc gcagtacata gttggcgccc agccagtcga agttcacctg agcgtcgctg
     4201 ccccacctgg ctgcggcgat ccagtcggct cgg
//
LOCUS       DAORNF010000234         4229 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_15609_length_4229_cov_1.610446, whole genome shotgun sequence.
ACCESSION   DAORNF010000234 DAORNF010000000
VERSION     DAORNF010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4229
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15609_length_4229_cov_1.610446"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="PLV68_10155"
     CDS             complement(<1..206)
                     /locus_tag="PLV68_10155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01653.1"
                     /translation="MTARDQAVFDRDLYRRLRATCTATIPRLLDATSTVAVDDHAPVT
                     AAELAPLAPTAAVARDAAAISGVDG"
     assembly_gap    222..311
                     /estimated_length=90
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<312..729)
                     /locus_tag="PLV68_10160"
     CDS             complement(<312..729)
                     /locus_tag="PLV68_10160"
                     /inference="COORDINATES: protein motif:HMM:NF016430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HRE01654.1"
                     /translation="MEARAAQPRVDASRFDAPLDPGFALGRGVDGDGDIDVAALYREH
                     ADRLRHLAAAITLDRWLAEEIAHDAFAGLHASAATIGNPLGYLQRAVVNRSVSALRRR
                     RTAVRHPDPVARPTVDPEIDETWDVVTALPARERAVV"
     assembly_gap    903..967
                     /estimated_length=65
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<968..1979)
                     /locus_tag="PLV68_10165"
     CDS             complement(<968..1979)
                     /locus_tag="PLV68_10165"
                     /inference="COORDINATES: protein motif:HMM:NF012457.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HRE01655.1"
                     /translation="MSTPVAVTFPDFAVACGEEASDPLVPYQGAIVRVDELAASGHLA
                     RQDADLADVAGLGVRWWRYGMPWRHTEPAPGEYDWTLWDQALAACDRHGLTPVVDLCH
                     FGLPDHLGGFCDPAWVDSFCRYVDAFLTRYREPMFFTPVNEPMITAMASALYGIWNDQ
                     RASRADFFRALANTTLANLEAIARIDADRGGWWIGAEGFGRHLAADADHEAEAIAANE
                     LERLVWDLHFGTEPAPSAHDVYEAVDPVLLDRIATRRVVTERVVVGHDFYPVSAHLHG
                     RRDEPLSIEERIAAYDLAAREWHARYQRPFWVAETSNLGLDVADGSRWLTELVACLDR
                     LRA"
     assembly_gap    2171..2181
                     /estimated_length=11
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2182..>2541)
                     /locus_tag="PLV68_10170"
     CDS             complement(<2182..>2541)
                     /locus_tag="PLV68_10170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01656.1"
                     /translation="VHQDLAPITFVHSDFAPSYETLVKTMYRRGEHDGSNTALARNGI
                     DASAVADARRIVILQFWRNIGPAKMDYPLGFCDARTIALDDVTAFPVDDYAGSGIDFE
                     ALGMHAPRPESRHRWYA"
     assembly_gap    2542..2649
                     /estimated_length=108
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2650..2858)
                     /locus_tag="PLV68_10175"
     CDS             complement(<2650..2858)
                     /locus_tag="PLV68_10175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01657.1"
                     /translation="MSGITSGVASPVRAFCRATLNFAGRGDIPALDTVEVEIIDGRSA
                     GLPGWETCGFELLAHRSAAPSWSDD"
     assembly_gap    2929..2998
                     /estimated_length=70
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2999..>4229
                     /locus_tag="PLV68_10180"
     CDS             <2999..>4229
                     /locus_tag="PLV68_10180"
                     /inference="COORDINATES: protein motif:HMM:NF014790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRE01658.1"
                     /translation="LPAPPPSAPATETAALRAEVRAWLGEHWPAGRDVPPKEWLGMVV
                     DARWAVPTWPPQWYGRGLSGKMAAVTLAEFRAAGAPGAGQDKYNLWANTLVAYGTDSA
                     KQRFVRRLLTDDLKMCLLYSEPGAGSDLAAVQTRADRDGDEWVVNGQKVWTSGAKEAD
                     YGMLIARTNWDVPKHGGISFFWLPMKDNGVEIRPILQITGESHFNEVFIDSARVPGDH
                     LLGPLDGGWRVLQTALAYERSVMGDMARGPRSTNKAARTAKADAARKSMAALDSATDS
                     ATDSATKPGADDESTFGEASQGAGVNLWKLAEQMGRLDDPVIRQRIAQVHIWRTVNTW
                     NGQRAKSQLAQGSSSPILSLGKLAMSRILHQAAHVQRMIVGQEAQLYGKHNPLGDDAN
                     FLSLNAYFTSIGGGTDQI"
BASE COUNT          621 a         1279 c         1413 g          572 t
ORIGIN      
        1 ccatccaccc cggagattgc agcagcgtcc cgagcgaccg cagcagtcgg cgcgagcggg
       61 gcgagctccg cagcggtgac cggtgcgtgg tcgtcaacgg cgaccgtcga tgtcgcgtcg
      121 agcagacggg ggatggtggc ggtgcaggtg gcccgtaggc ggcggtacag gtcgcggtcg
      181 aataccgcct gatcacgtgc ggtcatgaga gttcctttcg gnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nccacgacgg cccgctcgcg cgccggcagt gccgtgacca cgtcccaggt
      361 ctcgtcgatc tctggatcga cggtcgggcg cgcgacggga tcgggatggc gcacggccgt
      421 gcggcggcgg cgcagcgccg agacggagcg gttgacgacg gctcgctgca agtagccgag
      481 cggattgccg atggtcgcgg cgctggcgtg cagaccggca aacgcgtcgt gggcgatctc
      541 ttcggccagc caccggtcga gcgtgatcgc cgcggccagg tgccgcaggc gatcagcgtg
      601 ttcgcggtac agcgcggcga cgtcgatatc gccatcgcca tcgacaccac gccccagcgc
      661 gaagcccggg tcgagcggtg cgtcaaaccg tgacgcgtca acccgtggtt gggcggcccg
      721 tgcttccata ccccgacctg acgcccaacc gcccgactcg gttctcaccc cgccagcgtc
      781 gagtcagttc gcctgcgctg cggggtcagc gggtgcgggc gagggtggcg tagtcggcgg
      841 cgacggggcg ggggtggcgg tcggtgtcga acaggccgac ttccgtgacc tcgccggcgg
      901 ggnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      961 nnnnnnncgg cgcgcagtcg atcgaggcac gcgaccagct cggtgagcca gcgcgacccg
     1021 tcggcgacgt cgaggccgag gttcgacgtc tccgcgaccc agaacgggcg ttggtagcgg
     1081 gcatgccact cgcgggcggc gaggtcatag gcggcgatcc gttcctcgat cgacagcggt
     1141 tcgtctcgtc ggccgtgcag gtgagcgctg accgggtaga agtcgtgacc gaccactacc
     1201 cgctcggtga cgacgcgcct ggtcgcgatg cgatcgagca gcaccgggtc gaccgcctcg
     1261 tacacgtcgt gggcggaggg tgccggttcg gtgccgaagt gcaggtccca caccaaccgt
     1321 tccaactcgt tggccgcgat ggcttcggcc tcgtggtcgg catcggcagc gagatggcgg
     1381 ccgaaccctt cggcgccgat ccaccacccg ccgcggtcgg cgtcgatgcg ggcgatggct
     1441 tcgaggttgg ccagggtggt gttcgcgagg gcgcggaaga agtcggcccg gctggcgcgc
     1501 tggtcgttcc agatgccgta cagcgccgag gccatcgcgg tgatcatcgg ttcgttgacg
     1561 ggcgtgaaga acatcggttc gcggtagcgg gtcaggaacg cgtcgacgta gcgacagaag
     1621 ctgtcgaccc atgccgggtc gcagaaacca ccgagatgat cgggcaggcc gaagtggcac
     1681 agatcgacca ccggcgtcag cccgtggcgg tcacacgccg ccagcgcctg atcccacagc
     1741 gtccagtcgt actcgcccgg cgctggttcg gtgtgccgcc acggcatgcc gtagcgccac
     1801 caccgcacgc cgagcccggc gacatcggcc aggtcggcgt cctggcgggc caagtggccg
     1861 ctggctgcca gttcgtcgac gcgcacgatc gcgccttggt agggcacgag cgggtcggag
     1921 gcctcctcgc cgcaggcgac ggcgaagtcg ggaaaggtaa cagcaactgg ggtcgacatg
     1981 agcaggtatc aatcgtattg tgtcagagga acaggcacgt ggcgcgcagc tcgatgctct
     2041 ggcgcgccgg ctggccgatc gcaacggtcg ggtcgtggaa agccgagtgt ggggtgaagt
     2101 acacctcgcc ggcgcgggct ttgtccgaat cccacgtgcg gaacgcgacc acctcatccg
     2161 gttgcatctc nnnnnnnnnn naacgcgtac caacggtgcc gcgactcggg ccgcggcgcg
     2221 tgcatgccca acgcctcgaa gtcgatgcca ctgcccgcgt agtcgtcgac gggaaacgcg
     2281 gtgacgtcgt cgagcgcgat cgtgcgcgcg tcgcagaaac cgagtgggta gtccatcttt
     2341 gccgggccga tgttgcgcca gaactggagg atgacgatgc ggcgggcatc ggcaacggcg
     2401 gacgcgtcga tgccgttgcg ggccagcgcg gtgttggacc cgtcgtgctc gccgcggcgg
     2461 tacatcgtct tgaccagcgt ctcgtagctg ggcgcgaagt cggaatggac gaacgtgatc
     2521 ggtgccaggt cctgatggac gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnt cgtcgtcaga ccatgacggc gcggccgaac ggtgagcgag cagttcgaac
     2701 ccgcacgtct cccaacccgg taggccggcc gagcgcccgt cgatgatctc gacctcgacc
     2761 gtgtcgagtg cgggaatgtc gccgcgcccg gcgaagttca acgtggcccg acagaacgca
     2821 cggactggtg atgccacccc agacgtgatc ccagacatga gcggacccta cgcccgccgc
     2881 gcatggcgac gacgagccgt gcgcgcaact cgccgcctcg ctgcggctnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntt
     3001 gccggcgccc ccgccctccg ccccggcgac cgagacggcc gcgttgcggg ccgaggtacg
     3061 ggcctggttg ggcgagcact ggccggccgg tcgcgacgtg ccgcccaagg agtggctcgg
     3121 catggtggtc gatgcgcgct gggctgtacc gacgtggccg ccgcagtggt acggccgtgg
     3181 gctctcgggc aagatggccg cggtgaccct ggccgagttc cgtgccgctg gggcaccggg
     3241 tgccgggcag gacaagtaca acctgtgggc caacacgttg gtggcctacg gcaccgacag
     3301 cgccaagcag cggttcgtgc ggcggctgtt gaccgacgac ctcaagatgt gcctgctgta
     3361 ctccgagccg ggtgccgggt ccgacctggc cgcggtccag acccgggccg accgggacgg
     3421 cgacgaatgg gtggtcaacg gccagaaggt gtggacctcg ggcgccaagg aagccgacta
     3481 cggcatgctg atcgcccgca ccaactggga cgtccccaag catggcggca tctcgttctt
     3541 ctggctgccg atgaaggaca acggggtcga gatccgcccg atccttcaga tcacggggga
     3601 gagccacttc aacgaggtgt tcatcgacag cgcgcgagtg ccaggcgacc acctgctcgg
     3661 cccgctcgac ggcgggtggc gcgtgctgca gacggcgctg gcctacgagc gatcggtgat
     3721 gggtgacatg gcccgcgggc cgcgcagcac caacaaagct gcccgcacgg ccaaggccga
     3781 tgccgccagg aagtcgatgg cggcactgga ctcggccacc gactcggcca ccgactcggc
     3841 cacgaagccg ggcgccgacg acgagagcac gttcggcgag gccagtcagg gcgcaggtgt
     3901 caacctgtgg aagctcgccg agcagatggg ccgcctcgac gatcccgtga tccgtcagcg
     3961 gatcgcccag gtgcacatct ggcgcacggt caacacgtgg aacggtcagc gggccaagtc
     4021 gcagctggcc cagggttcgt cgtcgcccat cttgtcgctg ggcaagttgg cgatgtcgcg
     4081 catcttgcac caggccgccc acgtgcagcg gatgatcgtc ggccaggagg cgcagctcta
     4141 cggcaagcac aacccgctgg gcgacgacgc caacttcctg tcgctcaacg cttatttcac
     4201 ctcgatcggt ggcggtaccg atcagatcc
//
LOCUS       DAORNF010000235         4228 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_15617_length_4228_cov_1.845675, whole genome shotgun sequence.
ACCESSION   DAORNF010000235 DAORNF010000000
VERSION     DAORNF010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4228
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15617_length_4228_cov_1.845675"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            182..256
                     /locus_tag="PLV68_10185"
     tRNA            182..256
                     /locus_tag="PLV68_10185"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:215..217,aa:Cys,seq:gca)
     gene            385..903
                     /locus_tag="PLV68_10190"
     CDS             385..903
                     /locus_tag="PLV68_10190"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigE family RNA polymerase sigma factor"
                     /protein_id="HRE01659.1"
                     /translation="MPGEMNRPPGGVPTSLADGQADFEAFYRAEYAAAVRLARLLTDS
                     AAAAEDRAQDAFVRMYRHFARVDNPAAFLRTTVVNVCRSWHRSRRRETERLVKLTPVT
                     TEQAPVGRELDAVIAALSYKYRAVLVLRYWLDLSERDIAAALGCRPGTVKSTTSRALA
                     QLRTQLSAKELS"
     gene            900..2015
                     /locus_tag="PLV68_10195"
     CDS             900..2015
                     /locus_tag="PLV68_10195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01660.1"
                     /translation="MNGTILERPDPDDLEARLRSHFDAVAADTQVTVPARFQTEPMLR
                     LLGAQPPVESRRPVRWIAAAAAVAVAGGGLTVALWSGGSSTDTGGSAATDAALIGPAG
                     TLGPVLVTDPVLWMPDGSRSEIRITQLYEALTAPVATGAVIAPDGTVYSLLVTSIPST
                     DPIVSGASETHVVGGRTVTVRTETGLAGYTTRQFEQGCVTVSVSNGGGADGWGPDVEA
                     VLGAITLEGAATSLSLPIGWASLGAAETGHQYAVEFDVRLGGATRSVSMWQMPDASVG
                     FYLGMLAGSPVQLDPAAWAVDADPGEGLSGPVVVERDGTAFYLAGAATATELRQIADS
                     FVRAPLSAWTTARRDDVTAITTAGAAPGCAAPTLTIR"
     gene            complement(2135..3478)
                     /locus_tag="PLV68_10200"
     CDS             complement(2135..3478)
                     /locus_tag="PLV68_10200"
                     /inference="COORDINATES: protein motif:HMM:NF019352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HRE01661.1"
                     /translation="MSNEKPSFLSGGAFIFAAIAFLFAGFAILKASNDDGDGKALTDS
                     GGAVPTVDISLTEFAMSPAMPTVPEGKVTLRVTNNGTLVHNLAFSEGGITTRDLQPGE
                     SQTISVDAKGEMNFLCTIPGHSASGMTGHVMADPNATDVVIDDPMASMTWEEMDEAMA
                     ARGWRFVEEPKGTFGGQELEPTILADGTKQFELTASIIDWEVEPGKIVKAWTYNEMVP
                     GPTMRADVGDKIRVILHNELPESTTIHWHGMRVPNSMDGVDPYTQSPIKPGESFTYEF
                     EAVEPAVGMYHSHHNAQTQVPNGLMGAFYIGDMPIPDSLAEKGMTKVDQEITMVLNDA
                     GTIGLTLNGKSFPATQLYTAKLGDTVMVHYFNEGLTSHPMHLHQPVGWIIARDGHPLE
                     VPIPSDTINVAPGERITVLYHMEDPGVWAWHCHILNHAEGPLGMFGMVTAIIVEE"
     gene            complement(3590..>4228)
                     /locus_tag="PLV68_10205"
     CDS             complement(3590..>4228)
                     /locus_tag="PLV68_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRE01662.1"
                     /translation="VFLLDDHEVVRRGVRDLLESAGDIEVVGEASTAAEALARVPATN
                     PHVAVLDVRLPDGSGVEVCRDLLSAMPTLRCLMLTSFTDDEALFDAIMAGASGYVLKE
                     VRGSDLVDSVRRVAGGQSLLDPHVTAKVLERLRNPLTDERTASLTPQEQAILSHLAEG
                     LTNRQIGEKMYLAEKTVKNYVSNVLAKLGMSHRTEAAVYAARLADRRNQQRF"
BASE COUNT          651 a         1364 c         1470 g          743 t
ORIGIN      
        1 acctccgcgc ccgccagaac ctctccgtgg acgaagcggt cgtcacgttg caggcgctga
       61 tgcactcggt gttgcgctgc cgtgtgacca cggttccgtg accgatccag ccgcccggtt
      121 tgtcggggcc gaatcggcac gctgacgggt atcgtggccg accgatgccg cacggcatcc
      181 tggcgacgtg gccgaatggt tcaggcaagg gcctgcaaag ccctgtatcc gggttcgatt
      241 cccggcgtcg cctccagatc atcctcatat gcggactgcg gaggatgagc gaacctgcac
      301 cgttggtgga accgagttgg ggggtgcggg gtcggaatag gtgtgtcctc gtgcgacgac
      361 cgaccgactg aaacgctggg cgacgtgcca ggcgagatga accgaccacc gggtggggtg
      421 ccgacctcac tggcagacgg gcaggccgac ttcgaggcgt tctatcgcgc cgagtacgcc
      481 gcagcggtgc ggcttgcccg tctgttgacc gattccgccg ctgccgcgga ggatcgcgcc
      541 caggacgcgt tcgtgcgcat gtaccgccac ttcgcgcggg tcgacaatcc ggccgcgttc
      601 ttgcgcacca ccgtcgtcaa cgtgtgccgc agttggcatc gcagccgccg ccgcgagacg
      661 gagcggctgg tcaagttgac accggtcacc accgagcagg cacccgtcgg acgtgagctc
      721 gatgcggtga tcgcagcgtt gtcgtacaag taccgggccg tgttggtgct gcgctactgg
      781 ctcgatctca gcgaacgcga catagcggcc gcgctcggct gccgacccgg caccgtcaag
      841 tcgaccacct cgcgcgccct ggctcaacta cgcacacagt tatccgcaaa ggagttgtca
      901 tgaacggaac gattctcgag cgccccgacc cggacgacct cgaggctcgc ctgcgctcgc
      961 acttcgatgc agtcgcggcg gacactcagg tcaccgtgcc cgcacgcttc cagaccgagc
     1021 cgatgctgcg cctgctcggc gcgcaaccac cggtcgaaag ccgccgcccg gtgcgctgga
     1081 tcgcggctgc cgcagcggtc gcggtggccg gtggaggcct caccgtggcc ctctggagcg
     1141 gtggatcgtc caccgacacg ggcggttcgg ccgcgaccga cgcggctctg attggtcctg
     1201 caggcacgct gggaccggtg ctcgtgaccg acccggtgtt gtggatgccc gacgggtcac
     1261 ggtctgaaat ccgcatcacc cagttgtacg aagcgctcac cgctcccgtg gcaaccggtg
     1321 cagtgatcgc gccggacggg accgtttact cgctgctggt cacgagcatt ccgtccacgg
     1381 acccgatcgt gtcgggtgcc agcgagacgc acgtcgtcgg cggtcgcacc gtgacggtgc
     1441 ggaccgagac cggtctggcc gggtacacga cgcgccagtt cgaacagggc tgcgtcacgg
     1501 tcagcgtcag caacggtggt ggcgcggatg gttgggggcc cgatgtcgaa gccgtgctcg
     1561 gcgcgatcac cctggagggt gccgcgacca gcctgtcgct gccaatcggt tgggcgtcgc
     1621 tgggcgcggc cgagaccggc catcagtacg ccgtggagtt cgacgtgagg ctcggcgggg
     1681 cgacgcgttc ggtatcgatg tggcagatgc ccgatgcctc ggttgggttc tacctcggca
     1741 tgctggccgg ctcgccagtg caactcgacc cggccgcgtg ggcggtcgat gccgaccccg
     1801 gcgagggtct gtccggtccg gtggtcgtcg agcgcgacgg caccgccttc tacctggctg
     1861 gtgcggctac tgcgaccgag ttgcgccaga tcgccgacag cttcgtgcgc gccccgctgt
     1921 cggcgtggac gacagcacgc cgggacgatg tcactgcgat caccaccgct ggcgctgccc
     1981 ccggctgtgc cgcacccacc ctgacgattc gctgaggttc aggcagccca cgtggcgtga
     2041 cgagacgcga tgaaccccgg tccgggggtg acggaccggg gttcacgaac gatcgccagc
     2101 gtgcagcgat cacatcagcg tcaggagcga acgactactc ctcgacgatg atcgcagtca
     2161 ccatgccgaa catgccgagt ggaccttcgg cgtggttgag gatgtggcag tgccacgccc
     2221 acacgccagg gtcttccatg tggtaaagca ccgtgatccg ctcgccagga gcgacgttga
     2281 tcgtgtcgct ggggatcggg acctccagcg ggtggccgtc ccgggcgatg atccagccga
     2341 ccggctgatg caggtgcatc gggtgtgagg tcagaccttc gttgaagtag tggaccatca
     2401 cggtgtcgcc gagcttggcc gtgtacaact gggtcgccgg gaagctcttg ccgttgaggg
     2461 tcaagccgat cgtgcccgca tcgttgagca ccatggtgat ctcctgatcg accttggtca
     2521 tgcccttctc tgcgagcgag tcgggaatcg gcatgtcgcc gatgtagaag gcacccatca
     2581 ggccgttcgg cacctgggtc tgggcgttgt ggtgcgagtg gtacatgccc accgcgggtt
     2641 caaccgcttc gaactcgtac gtaaagctct cacccggctt gatcggcgac tgggtgtagg
     2701 ggtcgacgcc gtccatcgag ttgggcacgc gcataccgtg ccagtggatc gtggtcgact
     2761 caggcaactc gttgtgcagg atcacccgga tcttgtcacc gacgtcggcc cgcatggtcg
     2821 gccccggcac catctcgttg tacgtccagg ccttgacgat cttgcccggc tcgacctccc
     2881 agtcgatgat cgacgcggtc agttcgaact gcttggtgcc gtcggcgagg atcgtcggct
     2941 cgagctcctg gccgccgaag gtgcccttgg gctcttcgac gaagcgccac ccacgggcag
     3001 ccatcgcttc gtccatctct tcccaggtca tgctcgccat cgggtcgtcg atgacgacgt
     3061 cggtggcgtt cgggtcggcc atcacgtggc cggtcatgcc cgaggcgctg tggccgggga
     3121 tggtgcagag gaagttcatc tcgcccttgg cgtcgacgct gatcgtctgg ctctcgccag
     3181 gctgcaggtc acgggtggtg atgccgccct cggagaaggc caggttgtgc accaaggtgc
     3241 cgttgttggt gacccgcagc gtgaccttgc cctcgggaac ggtgggcatc gcgggggaca
     3301 tcgcgaactc ggtgaggctg atgtcgacgg tcggtaccgc gccgccggaa tcggtgaggg
     3361 ccttgccgtc gccatcgtcg ttgctcgcct tgaggatcgc gaacccggcg aacaggaacg
     3421 cgatggccgc gaaaatgaac gcgccgccgg acaggaacga tggtttctcg ttgctcatgc
     3481 gggcaagtat ggagatcgag cccggtcggt gtcgagtctc gggtcatcgt gattcgggac
     3541 caaagtccca agcggccacc gtggccctgg cgggcgggga cccgggcgct cagaaacgct
     3601 gttggttacg ccgatcggcg aggcgagcgg catacacagc ggcttcggtg cgatggctca
     3661 tgccgagctt ggccagcacg ttggagacgt agttcttgac cgtcttctcg gcgaggtaca
     3721 tcttctcgcc gatctggcgg ttggtgagcc cctcggccag gtgcgacagg atcgcctgct
     3781 cctggggggt cagcgacgcg gtgcgttcgt cggtgagggg gttgcgcagg cgttcgagca
     3841 ccttggcggt gacgtgaggg tcgagcagcg actggccccc ggcgacgcgg cgaacgctgt
     3901 cgaccaggtc ggacccgcga acttccttca gcacgtagcc cgaagccccg gccatgatcg
     3961 cgtcgaacag cgcctcgtcg tcggtgaacg atgtcagcat caggcaccgc aacgtcggca
     4021 tcgccgagag caggtcgcgg cacacctcga ccccgctgcc gtccggcagg cgcacgtcga
     4081 gtacggcgac gtgggggttg gtggccggga cacgggccag ggcttcggcc gcggtcgacg
     4141 cctcgccgac gacttcgatg tcgcccgcgc tctcgagcag gtcgcggacg ccacggcgaa
     4201 cgacctcgtg gtcgtcgagt agaaagac
//
LOCUS       DAORNF010000236         4227 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_15622_length_4227_cov_1.604506, whole genome shotgun sequence.
ACCESSION   DAORNF010000236 DAORNF010000000
VERSION     DAORNF010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4227
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15622_length_4227_cov_1.604506"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="PLV68_10210"
     CDS             <1..205
                     /locus_tag="PLV68_10210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01663.1"
                     /translation="PLPAVLIGAVRQDQTVVASLGWRTRLVRWSSTWAGVTEVPISLP
                     RRITVTGPSSLTTTTVAAGSSTL"
     gene            407..>1979
                     /locus_tag="PLV68_10215"
     CDS             407..>1979
                     /locus_tag="PLV68_10215"
                     /inference="COORDINATES: protein motif:HMM:NF037016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HRE01664.1"
                     /translation="MNSTAWRLRAAVILATVAAAPAMLASPAVADDVDVVLDNRNYYN
                     PCGTEFDGVSIGANADQYDGVDVDDWEFSLDGGTTWNPYYADEYATAPPDPKFGGKSY
                     YVLTQGASVALFRALDVNGNNYFGSVAIPSDISECSEDPDYTDILPLIGGALDDGPGL
                     ADDSFTVPTAVLGPMVFWNDTMAISNAAGGWTEWTPNVSMTLADLGYTNASVPPGGSL
                     IVYVKHSGGGTEVGSPIGDGAQYMNQATNNPDVDYLQEVSFGHGHLVFSNAQPSATDD
                     VATANGAPVTIAVIGNDTPIVGYPVESVSLLDSDANPVTSLITPEGTYTVVGLTVEFT
                     PAPGFSGTAPSVPYRITDTYGLTADATITVTVNVPPVANDDTATSNEGATVTIPVIGN
                     DTTQPGTTITNVTLLDSGGDPVTTLVTPEGTYTVVGLTVECSPTPGFTGTAPSVPYRI
                     TDTNGLTATATITVTVTASVVTTTSTTSTTSTTPTTSTDTTTPAGETPTGNLPVTGSD
                     SGGGLVLGAIVMLVGI"
     assembly_gap    1980..1995
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2144..>2392)
                     /locus_tag="PLV68_10220"
     CDS             complement(2144..>2392)
                     /locus_tag="PLV68_10220"
                     /inference="COORDINATES: protein motif:HMM:NF015279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01665.1"
                     /translation="GLRGLWDQVVAHRDHLDRTGHLALKRQRQSIDWMWESVDKHVVA
                     RFRARPDVRERAKSLEADLTAGRTTASLAASTLLALSQ"
     assembly_gap    2393..2583
                     /estimated_length=191
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2584..3115)
                     /locus_tag="PLV68_10225"
     CDS             complement(<2584..3115)
                     /locus_tag="PLV68_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRE01666.1"
                     /translation="MVTRAEPTLDQYVDGVLNADRAWLGRAITLVESTRPDHQALAAE
                     LLTALVPHSGRARRVGISGVPGAGKSTLIDALGTELTGDGHRVAVLAVDPSSTRTGGS
                     ILGDKTRMAALAADDNAFIRPSPSAGVLGGVTRATREAMVVVEAAGFDIVIVETVGVG
                     QNETAVAGMVDTFLLLM"
     gene            complement(3122..>3731)
                     /locus_tag="PLV68_10230"
     CDS             complement(3122..>3731)
                     /locus_tag="PLV68_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229973.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HRE01667.1"
                     /translation="GSGLDENLLALSIHAARARASVGEISAALEKVFGRHTAAIRTIS
                     GVYRSEIGESPSVTAARAAVDAFAEADGRRPRILIAKMGQDGHDRGQKVIATAFADLG
                     FDVDVGPLFQTPAEVARQAVEADVHAVGVSSLAAGHLTLVPQLRDELAAAGRPDILIV
                     VGGVIPEQDFDELRAAGAAAIFPPGTVIADSAIELLDKLRQA"
     assembly_gap    3732..3762
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3763..>4227)
                     /locus_tag="PLV68_10235"
     CDS             complement(<3763..>4227)
                     /locus_tag="PLV68_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HRE01668.1"
                     /translation="TNALDEAIALPTDFSARIARNTQLFLQQESGTCRVIDPWGGSYY
                     VEQLTNQLALRAWAHIQEVEAAGGMTEAITAGIPKLRIEEAAARTQARIDSGRQPVIG
                     VNMFRPTVPDSIEVLKVDNSAVRAQQIAKLERLRAERDQAACDAALDALTNA"
BASE COUNT          643 a         1457 c         1276 g          613 t
ORIGIN      
        1 ccctcttccc gccgtactga tcggcgccgt ccgccaggat cagacggtgg tcgcgtcgct
       61 cggctggcgc acgcggctcg tcaggtggag ctcgacgtgg gctggggtga ccgaggtgcc
      121 gatcagtctg ccgcgtcgga tcaccgtcac cgggccatcc tcactgacca ccacgaccgt
      181 cgccgcgggg tcgtcgacgc tgtagcggcg accggacgtg acgcattccc cggtagctgg
      241 cgacgtcggc acccgaactc acccgtcatc cgcgccgtgc cggccggatg gcgctgaacc
      301 acggcggctc caatgcacga aatggcgtca tcgcacggcc ttggctgcat ctacaaccac
      361 ggtggcctca ctcggggacc tccacgtgcc acgaaggaac acacgcatga actcgaccgc
      421 ttggcgtctc cgtgccgccg tcatcctcgc aaccgtcgct gccgctccag ccatgctggc
      481 ctcgcctgcc gtcgcggacg acgttgacgt cgtgctcgac aaccgcaact actacaaccc
      541 ctgcggtacc gagttcgacg gagtcagcat cggcgccaac gccgatcagt acgacggtgt
      601 cgacgtcgac gactgggagt tctctctcga cggcggcacc acgtggaatc cgtactacgc
      661 cgacgagtac gccaccgccc cgcccgaccc gaagttcggc ggtaagtcgt actacgtgct
      721 cactcagggc gcatccgtag cgttgttccg cgccctcgac gtgaacggca acaactactt
      781 cggctccgtc gccattccga gcgacatcag cgagtgctcc gaggaccccg actacaccga
      841 catcctcccg ttgatcggcg gcgcactgga cgacgggccg ggtctggccg acgactcctt
      901 caccgtcccc accgcggtac tcggcccgat ggtgttctgg aacgacacga tggccatctc
      961 caacgcggca ggcggctgga ccgagtggac gccgaatgtt tcgatgacct tggcagacct
     1021 cggctacacg aacgcctcgg ttcctcccgg cggatcgctg atcgtgtacg tcaagcactc
     1081 cggcggcggc accgaggtcg gctcgccgat cggggacggc gcgcagtaca tgaaccaggc
     1141 caccaacaac cccgatgtcg actatctcca ggaagtgtcg ttcggccacg gccatctggt
     1201 gttctccaac gcccaaccct cggcgaccga cgacgtagcc accgccaacg gggctcccgt
     1261 cacgattgcg gtgatcggca acgacacccc gattgtcggc taccccgtcg aatccgtgtc
     1321 cttgctcgac agcgacgcca accctgtgac ctcgttgatc acgccggaag gcacgtacac
     1381 cgtcgtcggg ctgacggtcg agttcacccc cgccccgggg ttctccggta ccgcgccgtc
     1441 ggttccgtac cgcatcaccg acacctacgg cctcaccgcc gacgccacca tcaccgtcac
     1501 cgtcaacgtc ccacctgtcg ccaacgacga cacggccacg tccaacgagg gtgccaccgt
     1561 cacgatccca gtgatcggca atgacaccac ccaacctggc accaccatca ccaacgtcac
     1621 cctgctcgac agcggcggag acccggtgac gacgctggtc actcccgagg gcacctacac
     1681 ggtggtcggc ctcacggtcg agtgcagccc cacccccggc ttcacgggca cggcgccctc
     1741 ggtcccctac cgcatcaccg acaccaatgg gctcacggcc accgcgacga tcacggtgac
     1801 ggtcaccgca tccgtcgtga cgacgacttc gacgacctcg acgacctcca cgacccccac
     1861 gacctcgacg gacacgacga ccccggcggg tgagacacca actggcaacc tgcccgtcac
     1921 cgggtctgac tcgggcggcg ggctcgtgct gggcgcgatc gtgatgctgg tgggcatcgn
     1981 nnnnnnnnnn nnnnncccgc cgcaaccccc gacgggccgt caccgcgtcg cgctgacaca
     2041 cacccgaacc gctgtgcggg atcgtgggca cgccccgccc acatcccgca ctgcggtcga
     2101 aacctggtac gacccgggac ggacgccgtg cggcgaccca gccttactgc gagagcgcga
     2161 gcagggtgct ggccgcgagg ctggctgtgg tgcgccctgc ggtgaggtca gcctccaacg
     2221 acttggcgcg ttcgcggacg tcggggcggg ctcggaagcg ggcgacaacg tgcttgtcga
     2281 cgctctccca catccagtcg atgctctgac gctgacgttt gagggcgaga tggccggtgc
     2341 ggtcgaggtg atcgcgatgg gcgaccacct gatcccacag accacgtagc ccnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnngcatcag cagcaggaac gtgtcgacca tgcccgccac cgcggtctcg ttctggccga
     2641 cccccacggt ctcgacgatc acgatgtcga agcccgccgc ctcgaccacc accatcgcct
     2701 cgcgggtggc gcgggtgacg ccacccagca cgcccgcgct cggcgacgga cggatgaagg
     2761 cgttgtcgtc cgcagccagc gcagccattc gggtcttgtc accgaggatg ctgccgcccg
     2821 tgcgggtgga cgacgggtcg accgccagca cggccacccg gtggccgtcg ccggtcaact
     2881 cggtaccgag cgcgtcgatc agcgtcgact tgccggcgcc gggcacgccg ctgataccga
     2941 cccgccgcgc ccgaccagag tgcggcacca gcgcagtcag cagttcggcc gccaacgcct
     3001 ggtgatcggg ccgggttgat tcgaccagcg tgatcgcccg acccagccac gcccggtcgg
     3061 cgttcagcac gccgtcgacg tactggtcga gggtcggctc ggcgcgtgtc accacggggg
     3121 ctcaggcctg gcgcagtttg tcgagcagtt cgatcgccga gtcggcgatg accgttccag
     3181 gtggaaagat cgccgccgcg ccagccgcgc gcaactcgtc gaagtcctgc tcggggatca
     3241 cgccaccgac cacgatcagg atgtcgggac gccccgccgc ggcaagctcg tcacgcagtt
     3301 gcggcaccaa cgtgaggtga ccggcagcca ggctggacac accgaccgcg tggacgtcgg
     3361 cttcgaccgc ctggcgggcg acttcggccg gggtctggaa caaggggccg acatcgacgt
     3421 cgaagccgag gtcggcgaac gccgtggcga tcaccttctg gccgcgatcg tggccgtcct
     3481 gtcccatctt ggcgatgagg atgcgcggcc gccgcccgtc agcttcggca aacgcgtcga
     3541 ccgcggcgcg ggccgccgtc acgctggggc tctcaccgat ctcgctgcga tacacacccg
     3601 agatcgtacg gatcgccgcg gtgtgacggc cgaacacctt ctccaacgcg gcgctgatct
     3661 cgccgacgct ggcccgcgcc cgggccgcgt ggatcgacag agcgagcagg ttctcgtcca
     3721 agccggagcc gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncggcgttg gtcagcgcat
     3781 cgagcgcggc gtcgcacgcc gcctggtcgc gctcggcccg cagacgttcg agcttggcga
     3841 tctgctgggc gcgcacagcg ctgttgtcga ccttgagtac ctcgatcgaa tcgggcacgg
     3901 tggggcggaa catgttgacg ccgatcaccg gctggcgacc ggaatcgatg cgggcctggg
     3961 ttcgcgcggc ggcctcttcg atgcgcagct tggggatgcc cgcggtgatc gcttcggtca
     4021 tgcctcctgc tgcttcgacc tcttggatgt gggcccacgc ccgcaacgcc agttggttgg
     4081 tgagctgctc gacgtagtag ctgccacccc acgggtcgat cacccggcag gtgccgctct
     4141 cctgctgcaa gaacagctgg gtgttgcggg cgatgcgggc cgagaagtcg gtgggcaggg
     4201 cgatcgcctc gtcgagggcg ttggtgt
//
LOCUS       DAORNF010000237         4219 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_15665_length_4219_cov_1.673871, whole genome shotgun sequence.
ACCESSION   DAORNF010000237 DAORNF010000000
VERSION     DAORNF010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4219
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15665_length_4219_cov_1.673871"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1508
                     /locus_tag="PLV68_10240"
     CDS             <1..1508
                     /locus_tag="PLV68_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HRE01669.1"
                     /translation="ALVASANLADRYISDRHLPDKAIDLVDEAGARLRIKRMGTPPDL
                     KELETKLTEVQEAKKKAVEEQRFEDAGRLRDQEKTLLEEKATREAEVKASGVDLFDEV
                     DEESVAEVLSIWTGIPVYKLTEEETAKLLHMEDELHKRIIGQENAIKAVSQSIRRTRA
                     GLKDPRRPSGSFIFLGPTGVGKTELAKTLTEFLFGDDSALISLDMSEYMEKHTVSRLV
                     GAPPGYVGYEEGGQLTEAVRRKPFSVVLFDEIEKAHPDVFNTLLQILEEGRLTDSQGR
                     TVDFRNTVLIMTSNLGTADLRKANVGFTKNDEAMSYERMKDKVMDALKGHFRPEFLNR
                     IDDVIVFHELAQNEVMQMVDLMTKRLVGQLESQGLGIELTQSARELLAQKGALSAKPA
                     EVLASCAATLGRRSALREDPLQGVPGWRDRGGRYRRRSRSSWRSPHRVPGGRRLPASA
                     HPGTRRSRRRRPRRRLTSDLGSGSAPATRSSRPTARWPARVAGPLRVVAPG"
     gene            complement(1505..>2552)
                     /locus_tag="PLV68_10245"
     CDS             complement(1505..>2552)
                     /locus_tag="PLV68_10245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01670.1"
                     /translation="FQPPSPRPETAGAQRPDAFQPPRPDSAGAQRVDSAQHASPQHAS
                     NAQHSGNAQHSGVQHSGPQSGAAQRVDAGHGDATSAHHFGEPGGSQMPAHQGHGAGGA
                     DNARRAANTARHAGRWHALAPTAKAGIIVGGVVAAGAIVVGTVAATRDDAQKTATPAS
                     EVVITEPTLDAAPAATVISVATSTTAVPTTTAAPTTTEAPTTTEAPTTTVATPAFESF
                     AGSYLMTAGDIQISGSVLNGSIPGGDTQTWVLTGVCDGVGECTISAEGGEVASTMGSI
                     GLPEGGTVTMTPAGPGSYTLTIEVPAESCGPAIGTMAVSYAGATLSGHFEISTSGGAC
                     PFATLTADFTGTRT"
     assembly_gap    2553..2609
                     /estimated_length=57
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2731..>2928)
                     /locus_tag="PLV68_10250"
     CDS             complement(2731..>2928)
                     /locus_tag="PLV68_10250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01671.1"
                     /translation="DTVDGRTPIIAEVRHVPEVIGLVLVWMLATPKRLAAPDVPSQRK
                     GSRLPQLSVIEGASDPRRRSS"
     assembly_gap    2929..2968
                     /estimated_length=40
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2969..>3312)
                     /locus_tag="PLV68_10255"
     CDS             complement(<2969..>3312)
                     /locus_tag="PLV68_10255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01672.1"
                     /translation="LDGCPSCRAFADDIHRVARAAAIAPAEATPDLTGHIVRAARASD
                     RRSVWWVLRLGLGVVAVQVIVLSAPGLLLGHAEGADAHTARHLGSFSIAYAIGLLVVA
                     LRPAKARGMLPL"
     assembly_gap    3313..3380
                     /estimated_length=68
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3705..3811
                     /estimated_length=107
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3812..4137
                     /locus_tag="PLV68_10260"
     CDS             <3812..4137
                     /locus_tag="PLV68_10260"
                     /inference="COORDINATES: protein motif:HMM:NF019886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HRE01673.1"
                     /translation="PVVSWLLSIARHVCADQVRRNQRRSRLATRLRAERPAASVDEHS
                     VHLQGLIDQLDPDRRQAFVLTQVLGLSYDEAAAACGCPIGTIRSRVARARADLMTAVR
                     AAQAQ"
BASE COUNT          634 a         1346 c         1368 g          599 t
ORIGIN      
        1 aggcgctggt cgcgtcggcc aacctggccg atcgctacat ctccgaccgt cacctgcccg
       61 acaaggcaat cgacctcgtc gacgaggccg gtgcccggtt gcggatcaag cggatgggca
      121 ccccacccga cttgaaggag ctggagacca agctgaccga agtgcaagag gccaagaaga
      181 aggcggtcga agagcagcgc ttcgaagacg ccggccgcct gcgcgatcag gaaaagaccc
      241 tgctcgaaga gaaggccacg cgcgaggccg aggtcaaggc cagcggtgtc gacctgttcg
      301 acgaggtcga cgaagagtcg gtcgccgagg tgctcagcat ctggaccggc atccccgtct
      361 acaagctgac cgaagaggag accgccaagc tgctccacat ggaagacgag ctgcacaagc
      421 ggatcatcgg ccaggagaac gcgatcaagg cggtcagcca gtccatccgc cgtacccgtg
      481 ccgggctcaa ggacccccgc cgtccctccg gctcgttcat cttcctcggc cccaccggtg
      541 tcggcaagac cgagctggcc aagacgctca ccgagttctt gttcggcgac gacagcgcgc
      601 tgatcagcct cgacatgtcc gagtacatgg agaagcacac ggtcagccgt ctggtcggcg
      661 cgcctcccgg ctacgtcggc tacgaagagg gcggccagct caccgaagcc gtgcggcgca
      721 agccgttcag cgtggtgctg ttcgacgaga tcgaaaaggc tcacccggac gtgttcaaca
      781 cgttgctcca gatcctggag gaaggccgtc tcaccgacag ccagggccgc acggtggact
      841 tccgcaacac cgtgctgatc atgacgtcca acctcggcac cgccgatctg cgcaaggcca
      901 acgtcgggtt caccaagaac gacgaggcga tgagctacga gcggatgaag gacaaggtga
      961 tggacgccct caagggtcac ttccgtcccg agttcctcaa ccgcatcgac gacgtgatcg
     1021 tgttccacga actcgcccag aacgaggtga tgcagatggt cgacctgatg accaagcgcc
     1081 tcgtgggtca gttggagagc cagggtctcg gcatcgagct cacccagtcg gctcgcgaac
     1141 tgctcgccca gaagggcgcg ctctccgcga aacccgccga agtcctcgcg tcgtgcgctg
     1201 caacgcttgg tcgaagatcc gctctccgag aagatcctct acaaggagtt ccgggctggc
     1261 gagatcgtgg tggtcgatac cgaagacgat cccgatcgtc ctggcgaagt ccgcatcgcg
     1321 ttccgggcgg tcgaaggctt ccagcctccg cccaccccgg aactcgccgg agccggagac
     1381 gccggccccg ccgccgactg acctccgatc tcggatccgg ttcagcaccg gcaacccgct
     1441 ccagtcgtcc aacggctcgg tggcccgctc gggtcgccgg gccgttgcgc gttgtcgctc
     1501 ctggttaggt gcgggtgccg gtgaagtcag cggtgagtgt ggcgaacggg cacgccccac
     1561 cgctggtgga gatctcgaag tggccgctga gggtggcgcc cgcgtagctg actgccatcg
     1621 tgccgatcgc cgggccgcag ctttcggcgg gtacttcgat cgtcagcgtg taactgccgg
     1681 ggccggcggg ggtcatcgtc acggtgcccc cttcgggcag gccgatcgac cccatcgtgc
     1741 tcgcgacctc gccgccttcg gcgctgatgg tgcactcacc gacgccgtcg cagacgccag
     1801 tgagcaccca ggtctgcgtg tcgccccccg ggatcgaacc attgagcacc gagcccgata
     1861 tctggatgtc gccggccgtc atcaggtagc tgcccgcgaa cgactcgaac gccggggttg
     1921 caacggtggt ggtcggtgcc tcggtcgtgg tgggcgcttc cgtcgtggtc ggtgctgcgg
     1981 tggtggtggg gacggccgta gtcgacgtcg ccacgctgat gacggtcgcc gccggggccg
     2041 cgtcgagcgt cggctcggtg atgacgactt cgctcgccgg tgtcgcggtc ttttgcgcgt
     2101 cgtcgcgcgt ggcggcgacg gtgccgacca cgatcgcccc agcggcaacg accccgccga
     2161 cgatgatgcc tgccttggcc gtcggcgcga gggcgtgcca gcgcccggcg tgacgagcgg
     2221 tgttggcggc ccgacgggcg ttgtcggcac ctccagcacc gtgaccctga tgggctggca
     2281 tctgactgcc gcctggctcg ccgaagtgat gggccgaggt cgcgtcgccg tgaccggcat
     2341 cgacccgctg cgcggcgccc gactgcgggc cactgtgctg cacgccggag tgctgggcgt
     2401 tgccggagtg ctgggcgttg ctggcgtgtt gcgggctggc gtgttgggcc gagtcgaccc
     2461 gctgggcacc ggcactgtcg ggacgcggcg gctggaacgc gtctggtcgt tgggcacccg
     2521 ctgtctcggg gcgcggtgac ggcggctgga acnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnng ggcttgcgga ttgggcagtt gaggggtggg
     2641 gaactgcggt gtctggggtg gagtcgccgc gttggggtgg tcgctcatgg tgggcctcgc
     2701 gccggggatt cgtggtccgg cgcggcaact ctacgacgag cgacggcgag ggtcggacgc
     2761 gccctcgatc acactcaact gcggtaaccg cgaacccttg cgttgactcg ggacgtcggg
     2821 cgcagcgagg cgcttcggcg tcgccaacat ccacaccagc accaagccga tcacctcggg
     2881 cacgtggcgg acctcggcga tgatcggtgt ccgcccatcg acagtgtcnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnncc aacggcaaca tgccgcgagc cttggccgga
     3001 cgcagcgcca cgaccagcag cccgatggcg taggcgatgg agaacgagcc gaggtgacgc
     3061 gctgtgtgcg catcggctcc ctcggcgtgt cccaacagca ggccgggagc cgacaacacg
     3121 atcacctgca cggcgaccac gcccaggccg agccgcagca cccaccacac cgaacgacgg
     3181 tcgctggccc gcgcggcgcg cacgatgtgg ccggtcagat cgggggtcgc ctcggccggg
     3241 gcgatcgccg ccgcccgcgc gacgcggtgg atgtcgtcag cgaaggcccg acacgagggg
     3301 cagccgtcga ggnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn ccatccacac atcacacgac acacccattg gtcgccgccc
     3421 accccacgtc agttcccaca aacctccacg cctccaggac gcggctcacc gatagacgtc
     3481 gcgacgatgc cgatgcccgg cacgctcgac gctcgctcga cccgcagacg gacgatcacg
     3541 accagcccgg acagggctga cagtcgtcac gcttctgggt gtggcggacg ccgcggcggt
     3601 aggttggcgg ggatgcgtcc gggtgacaac gaggtgctgg tgcggtatgt cgaggccgca
     3661 cgcaacggcg acgacgtcgc tctcgggcgg ttggtgcaag cgacnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncgccggtgg tgtcgtggct gttgtcgatc
     3841 gcccgccatg tctgcgccga tcaagtgcga cgcaaccaac gccggtcccg cctggccacc
     3901 cggctgcgcg ccgaacggcc tgcagccagc gtcgacgagc actcggtgca cctccagggc
     3961 ttgatcgacc aactcgaccc cgatcggcgc caggcgttcg tgctcaccca ggtgctcgga
     4021 ctgtcctacg acgaggctgc cgccgcctgc ggctgcccca tcggcacgat ccgctcgcgg
     4081 gtcgcccgcg cccgcgccga tctgatgacc gcggtccgcg ccgcgcaagc ccagtagttc
     4141 tcgcgctgtg aacgcctggg aggcgatcgc cgtggtcgcg gccggcaccg ctgcgggtgg
     4201 gatcaacgcc atcgtcggg
//
LOCUS       DAORNF010000238         4201 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_15779_length_4201_cov_1.804149, whole genome shotgun sequence.
ACCESSION   DAORNF010000238 DAORNF010000000
VERSION     DAORNF010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4201
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15779_length_4201_cov_1.804149"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..976)
                     /locus_tag="PLV68_10265"
     CDS             complement(<1..976)
                     /locus_tag="PLV68_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HRE01674.1"
                     /translation="MLTNESVLEALRPVEDPELHRSIVDLGMVRSVALGDDGSVKVLV
                     ALTVPGCPLRNEIQNRVSGALEAIGATAVAIDFTVMTDAERERVRSLVHGNPGATAGH
                     GHAHGHAEGRAIPFADAGSKTRPLLISSGKGGVGKSSVTVNLAVALAAQGYSVGVVDA
                     DIYGYSVPRMLGTDRDPVAIDSMLVPPEQWGVRCISIGYFVPEGQAVIWRGPMLHKAL
                     EQFLTDVYWDEPDFLLIDMPPGTGDIALSLSQYLPRGEVYVVTTPQPAAQEVARLSAA
                     MAAKVNLPVKGVIENMSWFTGDDGQRYELFGAGGGQKLADDLDVPLLGQ"
     gene            1040..1819
                     /locus_tag="PLV68_10270"
     CDS             1040..1819
                     /locus_tag="PLV68_10270"
                     /inference="COORDINATES: protein motif:HMM:NF037269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRE01675.1"
                     /translation="MTSPPVPRSQPARRDAGQGTTSRPAGAHSFSAQVSALTTAFGDP
                     TRRGIYLFAREHRNGVTAAQVAEHFGVHPNVARHHLDKLVAGGYVEVAVERVEGGGAG
                     RPSKHYSAEPFDFPVRSDDLVLTLLARALAMLPREQAEAMAEEIGAEYGHSMALGAAV
                     ANDGPATNETAQLSFRNALHTVAEALTAHGFAAHADQRNNQLRIVNNHCPFGDHAIDN
                     PVICAVDRGMVKGMLKALYGDTDPSIEASLPQGDRVCVTSF"
     gene            1882..2649
                     /locus_tag="PLV68_10275"
     CDS             1882..2649
                     /locus_tag="PLV68_10275"
                     /inference="COORDINATES: protein motif:HMM:NF025580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4328 domain-containing protein"
                     /protein_id="HRE01676.1"
                     /translation="MSEFPNDSAFPKPPPPPDMAPPPGYVGYGAAPTPGYGQYRRIGG
                     QAKALRVLLAIFVVASVISLALLPSAKSKALAYVNGTGSLSDYEDALGPWSLANSLVG
                     GVTVAIAVLVMIWMNRMTKNLQLIGRVGTWTPGWAIGGWFLPPVLFVIPYLMLRDLWK
                     GSDPQSQPNWRSNPVAPIVHVWWALYGVIPLAFVTVTFSRFSFSFDNDNIDQAKNLSD
                     SFGITMAAQAIQIGAAVALLLLVTQLTKRHTEITREA"
     gene            2649..3119
                     /locus_tag="PLV68_10280"
     CDS             2649..3119
                     /locus_tag="PLV68_10280"
                     /EC_number="5.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase family protein"
                     /protein_id="HRE01677.1"
                     /translation="MLFLVRHAKAGQRDDNDPHDDQRELTTKGWAQAHALVEPLIAAG
                     ATTVVASPFIRCRQTVQPLADALGGEVAIDDRLAEDQPFSPVLALLSQVPDGTVLCSH
                     GDVIPETVSALIRRGCRLTTEPNWQKASVWLLDRDSDGAIVTAASWPPPHVLAP"
     gene            3460..>3831
                     /locus_tag="PLV68_10285"
     CDS             3460..>3831
                     /locus_tag="PLV68_10285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01678.1"
                     /translation="MHQGTANRRSSTATSNHAPAGYQPYGAFAPAAKTSGKLIAAGVM
                     EFIQAAFFVLVGILLIGVSNDVDRTFGFGGGSVAAVGVVVLAIGGALIAAGVGAVRGR
                     QWGRIFTIVLQSLALLLFIASL"
     assembly_gap    3832..3903
                     /estimated_length=72
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3945..>4201)
                     /locus_tag="PLV68_10290"
     CDS             complement(3945..>4201)
                     /locus_tag="PLV68_10290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01679.1"
                     /translation="LTDPVADAVQAIELALAVDAGYAEGHCARAIVAALSEDDDAAAT
                     SHRGECAALGAGGALLDATDAAIDTGAFDLAVFAAADSPG"
BASE COUNT          673 a         1456 c         1372 g          628 t
ORIGIN      
        1 gttggccgag cagcggcacg tcgagatcgt cggccagctt ctggccacca cccgcgccga
       61 acagctcgta gcgctggcca tcgtcgcccg tgaaccagct catgttctcg atcacgccct
      121 tgaccgggag gttgaccttg gccgccatgg ccgccgagag gcgagcaacc tcttgggccg
      181 cgggctgcgg cgtggtgacg acatagacct cgccgcgggg caggtactgg ctgagcgaca
      241 atgcgatgtc gccggtgccg ggcggcatgt cgatcagcag gaagtcgggc tcgtcccagt
      301 agacgtcggt gaggaactgt tcgagcgcct tgtgcagcat cggcccgcgc cagatgaccg
      361 cctggccttc gggcacgaag tagccgatcg agatacagcg cacgccccac tgctcgggtg
      421 gcacgagcat ggagtcgatc gccacggggt cgcgatcggt gccgagcatg cgcggcacgc
      481 tgtagccgta gatgtcggcg tcgaccacac cgaccgagta gccctgcgcg gccagcgcga
      541 ccgcgaggtt gaccgtgacg ctgctcttgc cgacgccgcc cttgcccgag ctgatcagca
      601 gcggccgcgt cttcgacccg gcgtcggcga acgggatcgc ccggccctcg gcgtggccgt
      661 gagcatgccc gtgtccagca gtggcaccgg ggttgccgtg aaccaggctg cggacccgct
      721 cacgctcggc atcggtcatg accgtgaagt cgatggcgac cgcggtggca ccgatcgctt
      781 cgagcgcccc ggaaacacga ttctggatct cgttgcgcag gggacagcct ggcacggtga
      841 gcgccaccag caccttcacc gaaccgtcgt caccgagggc gacgctgcgg accatgccga
      901 gatcgacgat cgaacgatgc aactcggggt cttcgaccgg acggagggct tcgaggacgg
      961 actcgttggt caacatgtgg gctccggtgg gttcggcaac cgcaggaatt actcctacgc
     1021 cgataggtag aataccggcg tgacctcccc gcccgtcccc cgcagtcagc ccgctcggcg
     1081 tgacgccggt caaggcacga ccagtcggcc agcgggcgcc cactcgttca gcgcccaggt
     1141 gtccgccctc accacggcgt tcggtgaccc gacccggcga ggcatctacc tgttcgcccg
     1201 cgagcaccgc aacggtgtca ccgcagcgca ggtcgccgag cacttcgggg tgcatcccaa
     1261 cgtcgcccgc caccacctcg acaagctggt cgccgggggc tacgtcgaag tcgcggtcga
     1321 gcgcgtcgaa ggcggcggcg ccgggcggcc gtcgaagcac tacagcgccg agccgttcga
     1381 cttccccgtg cgcagcgacg acctggtgct caccctgctg gcccgcgcgc tggcgatgct
     1441 gccgcgcgaa caggccgaag cgatggccga agagatcggt gccgaatacg gccactcgat
     1501 ggccctcggt gccgcggtcg ccaacgatgg cccggcgacc aacgagaccg cgcagctgtc
     1561 gttccgcaac gcgctgcaca cggtggccga agcactcacc gctcacggct ttgcggccca
     1621 cgccgaccag cgcaacaacc agctgcgcat cgtcaacaac cactgcccat tcggcgacca
     1681 cgcgatcgac aacccggtga tctgcgccgt cgaccggggc atggtcaagg gcatgctcaa
     1741 ggcgttgtac ggcgacaccg acccgtcgat cgaagcgtcg ctgccccaag gcgaccgggt
     1801 ctgcgtcacc agcttctgac gccggcgtca cgggcacgcg ccaggcccgc cccctcgtgt
     1861 gccaacccct agtgtgacgg gatgagcgag ttcccgaacg actccgcgtt tcccaagcct
     1921 cccccgccgc cggacatggc gccaccgccc ggctacgtcg gctacggcgc agcaccgaca
     1981 ccgggatacg gccagtaccg gcgcatcggc ggccaggcca aagcattgcg agtgttgctc
     2041 gcgatcttcg tcgtcgccag cgtgatctcg ctcgcgttgc tgccgagcgc gaagagcaag
     2101 gcgctcgcct acgtcaacgg cacgggcagc ttgagcgact acgaagatgc gctcggcccg
     2161 tggagcctgg ccaactcgtt ggtcggcggt gtcaccgtcg cgattgcggt gctggtgatg
     2221 atctggatga accggatgac caagaacctg cagttgatcg gccgggtcgg tacctggacg
     2281 ccggggtggg caatcggtgg gtggttcctg ccgcccgtgc tgttcgtgat cccttacctg
     2341 atgttgcgcg acctatggaa gggatccgac ccacagtcgc agcccaactg gcgttcgaac
     2401 ccggtggcac cgatcgtcca cgtgtggtgg gcgctctacg gcgtgatccc gctggcgttc
     2461 gtgacggtga cgttcagccg cttttcgttc agcttcgaca acgacaacat cgaccaggcc
     2521 aagaacctca gcgactcgtt cggcatcacg atggccgccc aggcgatcca gatcggcgcg
     2581 gccgtcgcat tgctgttgct ggtgacacag ctcaccaagc ggcacaccga gatcacccgc
     2641 gaggcctgat gctgttcctc gttcgccacg ccaaggcggg acagcgcgac gacaacgatc
     2701 cccacgacga ccagcgcgag ctgaccacca agggctgggc gcaggcccat gcgttggtcg
     2761 agccgctgat cgccgctggg gcgaccacgg tggtggcgag cccgttcatc cgttgtcgcc
     2821 agaccgtgca accactggcc gacgcgctgg ggggcgaggt cgcgatcgac gaccggctgg
     2881 ccgaagatca gccattctct cccgtgctcg cactgttgag tcaggtgccc gacggcaccg
     2941 tcttgtgcag ccacggcgac gtgatcccgg agacggtcag cgcgctgatc cgccgcggct
     3001 gtcgcctgac caccgaaccc aactggcaga aggcgtccgt gtggctgctc gaccgcgact
     3061 ccgacggcgc gatcgtcacc gccgcctcct ggccgccacc gcacgtcttg gcaccgtgag
     3121 ttcttgcacc gtgagtcctg acattgtgag tcctggccgc gcggcgcgcg actagcgtgc
     3181 acgtcatgtc gggacaatgg gcagcagatc cgtacggacg ggccgaactt cgctactggg
     3241 atggctcggt gtggaccagc cacgtctcga ccggcggcgt cgtcagcgac gagagccaac
     3301 ccgcagcaag caccggctgg ccgccagcgc aggagcaacc gcgccaggcc ttcgagcaac
     3361 ccacgcaggg gtacggccag acggctcaac ccttcggcca gccccagcag ttcagccagc
     3421 cccagcagtt cagccaaccc agtcagggct acgggcaggg tgcaccaggg tacggccaac
     3481 cggcgcagca gtacggctac cagcaaccac gccccggccg ggtaccagcc ctacggcgcg
     3541 ttcgcgcccg cggccaagac cagcggcaag ctgatcgccg ccggagtgat ggagttcatc
     3601 caggccgcgt tcttcgtgct ggtcggcatc ttgctgatcg gcgtgtccaa cgacgtcgac
     3661 cgcaccttcg gcttcggcgg tggttcggtc gcagcagtcg gcgtcgtcgt cctggccatc
     3721 ggtggtgcgc tgatcgcggc cggcgtcggt gccgtgcgcg gccggcagtg ggggcgcatc
     3781 ttcaccatcg tgttgcagtc gctggcgctg ctgttgttca tcgcctcgct gnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 nnnaggccaa gcagtaagga tcaggcgcgg ccgaccagcg cgtatcagcc gggcgaatcc
     3961 gccgcggcga agaccgcaag gtcgaacgcc ccggtgtcga tcgcggcatc ggtcgcgtcg
     4021 agcagcgcac cgcctgcgcc gagcgcggcg cactcgccgc ggtgagacgt cgcggccgca
     4081 tcgtcgtcct cggacagcgc ggccacgatc gcccgggcgc agtgcccttc ggcgtagccc
     4141 gcatcgaccg cgagcgccaa ctcgatcgcc tgcaccgcat ccgccaccgg gtcggtcagg
     4201 t
//
LOCUS       DAORNF010000239         4172 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_15923_length_4172_cov_1.895555, whole genome shotgun sequence.
ACCESSION   DAORNF010000239 DAORNF010000000
VERSION     DAORNF010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4172
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15923_length_4172_cov_1.895555"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>285)
                     /locus_tag="PLV68_10295"
     CDS             complement(<1..>285)
                     /locus_tag="PLV68_10295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01680.1"
                     /translation="LPPVPMGVPDVPLEPPWVGDEPTLPDLDRTRDATAVQPFEHIDR
                     GGRPSRLISEETFQAIRRNLGRTALAVVMALPVGAVVFWGWASARAAFDDR"
     assembly_gap    286..380
                     /estimated_length=95
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <381..>1519
                     /locus_tag="PLV68_10300"
     CDS             <381..>1519
                     /locus_tag="PLV68_10300"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HRE01681.1"
                     /translation="GATAGGTTNQTTTGAGSDRALPWWRGAVGYEIYVRSFQDSNGDG
                     TGDLAGITSRLGHLADLGIDVVWITPFFPSPGKDHGYDVSDYCDVDPQFGTLADFDAL
                     IAAAHARGIRVLADIVPNHSSDQHPWFQAAIADVASPYRDYYHFRPAAPDGGPPNNWV
                     ANFGGSAWSPDPAGTGMYYLHLFLPEQPDLNWTNPAVMDEFLAILRFWCDRGVDGFRI
                     DVAHGLTKDPEFHDNIQLRPVTADMHPAAVYWSFEHVNDSNRPETAEIFRRWHAEVAP
                     YGAVLFGEIGTPDPAGVAHAVAQHDALDGGFILRISAMKWDPAKIVDKLVAFNEASRG
                     GAVWALSNHDISRAVSRFGAATVGQQRAVDRVVTVTTLLIALDGI"
     assembly_gap    1520..1624
                     /estimated_length=105
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1625..2350
                     /locus_tag="PLV68_10305"
     CDS             <1625..2350
                     /locus_tag="PLV68_10305"
                     /inference="COORDINATES: protein motif:HMM:NF023367.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HRE01682.1"
                     /translation="RSGMAWDGTAHNGFTTAATAWLETAPVPLAMTVAGQADDPHATL
                     ATYRELIALRHRFPQLHQAPLAVVERTGTLLVLRRADLTIVANLAPEPVEVEIGGARR
                     RAPHRVREQARRSTDRRTGRGPAVACRTHRWRTGRRHPDWDVMTTAMDLAELQAVIET
                     TFGERDRARGVSATVAWLAEEVGELAQAVRKGTHDQRVHEFGDVIAWVATLANQVGVD
                     LTEAIDRYRTGCPRCQSSPCACP"
     gene            2350..2892
                     /locus_tag="PLV68_10310"
     CDS             2350..2892
                     /locus_tag="PLV68_10310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01683.1"
                     /translation="MADPDDPTTSAGGPTAPLPDDRIALRANATKWVWVTLGSLVFVL
                     AGGVMVLSETTGGVAKALGWVLVLLFGMCLVVALRQMINPGALIIGSDSIETLARRRL
                     TTYELRQCGPFGTWRNPSRNSWHVVFDYRQDSDDDPIHRANRQLMGGSRVLFEHYGVP
                     AEELAEMLERARQRALTADA"
     gene            complement(2786..3937)
                     /locus_tag="PLV68_10315"
     CDS             complement(2786..3937)
                     /locus_tag="PLV68_10315"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012784201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HRE01684.1"
                     /translation="MRVGIVGATGQVGGVMRAILAERKFPVTELRYFASARSAGITLP
                     WLDGEITVEDAAIADPTGLDIALFSAGATSSKELAPKFAAAGVTVIDNSSAFRMDPDV
                     PLIVSEVNGHLARQAPKGIIANPNCTTMAAMPVLKPLHDEAGLVRLIAATYQAVSGGG
                     LAGVDELDKQVREVVDTARELTYDGAAVSMPAPAKFAKPIAFNVLPYAGKLVDDGLFE
                     TDEEQKLRNETRKILDIPGLAVSGTCVRVPVFTGHSLSINAEFARPLSVARAIELLAA
                     APGVIVTDVPTPLEAAGRDASYVGRIRVDEGVADGRGLALFLSNDNLRKGAALNASCS
                     SPERPSVLTGRAPGLRRPRSVPAGGLVRASRPVLPRVRRSARTALASHP"
BASE COUNT          649 a         1383 c         1348 g          592 t
ORIGIN      
        1 cgatcgtcga acgcggcgcg agccgacgcc caaccccaga acacgaccgc gccgaccggc
       61 agcgccatca ccaccgccag cgccgtacgg ccgaggttgc gtcggatcgc ctggaaggtc
      121 tcctcggaga tcaaccgact cggccgtcca ccacggtcga tgtgctcgaa cggttggacg
      181 gcggtggcat cgcgcgtccg atcgaggtcg ggcaacgtcg gctcatcgcc gacccacggc
      241 ggctccaggg gaacgtcggg cacgcccatc ggcaccggag gaagcnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn caggcgccac ggcgggcggc acgacgaatc agaccaccac
      421 tggcgccggg agcgaccgcg cgttgccgtg gtggcgcggt gcagtcggct acgagatcta
      481 cgttcgcagc tttcaggaca gcaacggtga cggcacgggc gatctggccg gcatcacgtc
      541 gcggctcggc catcttgccg acctgggcat cgacgtcgtc tggatcacgc cgttcttccc
      601 cagccccggc aaggaccacg gctatgacgt cagcgactac tgcgatgtcg acccgcagtt
      661 cggcacgctg gccgacttcg acgcgctgat cgccgccgcc cacgcgcggg gcatccgggt
      721 gttggccgac atcgtgccga accacagctc cgatcagcat ccgtggttcc aagccgcgat
      781 cgccgacgtc gccagcccgt accgcgacta ctaccacttc cgccccgcag cgcccgacgg
      841 cggtccgccc aacaactggg tcgccaactt cggcggctcg gcgtggtcgc ccgatccagc
      901 gggtacgggc atgtactacc tgcacctgtt cctccccgaa cagcccgacc tcaactggac
      961 gaaccccgcg gtgatggacg agttcctggc gatcttgcgc ttctggtgcg atcgtggcgt
     1021 cgatgggttt cgcatcgacg tcgcccacgg cctgaccaag gaccccgagt tccacgacaa
     1081 catccagttg cgccctgtca ccgccgacat gcaccccgca gcggtgtact ggtcgttcga
     1141 acacgtcaac gactcgaacc ggccggagac ggccgagatc ttccgccgct ggcacgccga
     1201 agtcgcgccg tacggcgccg tactgttcgg cgagatcggc acgcccgacc cggccggcgt
     1261 cgcccacgcc gtcgcccagc acgatgcgct cgacggtggc ttcatcttgc gcatcagcgc
     1321 catgaagtgg gacccggcca agatcgtcga caagttggtc gccttcaacg aggcaagccg
     1381 cggcggggcg gtgtgggcgc tgtcgaacca cgacatctcc cgggcggtca gccgcttcgg
     1441 cgcggcgacc gtcggccagc agcgagcggt cgatcgcgtc gtcacggtga cgacgctgct
     1501 gatcgcgctc gacggcatcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnncgcagc ggcatggcct gggacggcac ggcccacaac gggttcacca ccgccgcgac
     1681 cgcgtggctc gagaccgcgc cagtgcccct ggccatgacc gtggcgggcc aggccgacga
     1741 cccgcacgcg acgttggcca cctaccgcga actgatcgcg ctgcgccacc gcttccccca
     1801 actgcaccag gctccgctgg cggtggtcga gcgcaccggc acgctgttgg tgctgcgccg
     1861 cgccgatctg acgatcgtgg ccaacctcgc accggaaccg gtcgaggtcg agatcggcgg
     1921 agcgcggcga cgagcaccgc atcgtgttcg agagcaggct cggcgcagca cagatcgccg
     1981 aaccggccgc ggaccggccg tcgcgtgtcg tacacatcgg tggcgaacgg gccgccgtca
     2041 tccagactgg gacgtgatga cgacagcgat ggacctcgcc gaactgcaag ccgtgatcga
     2101 gacgaccttc ggtgagcgcg atcgggcccg gggggtatcg gccacggtgg cctggctggc
     2161 cgaagaggtc ggcgaattgg cccaggcggt gcgcaaaggc actcacgacc aacgcgtcca
     2221 cgagttcggc gacgtcatcg cctgggtcgc caccttggcg aaccaggtgg gcgtcgacct
     2281 gaccgaagcg atcgaccgct accgcaccgg ctgccctcgc tgccagtcgt caccctgcgc
     2341 gtgcccgtga tggccgaccc cgacgacccg acgacgagcg cgggcggacc gacagcaccg
     2401 ctgcccgacg accgcatcgc gctgcgcgcc aacgccacca agtgggtgtg ggtcacgctc
     2461 ggcagcctgg tcttcgtgct ggccgggggc gtgatggtgc tcagcgagac cacgggcggc
     2521 gtggccaagg cacttggctg ggtgctggtg ctgctgttcg gcatgtgcct ggtggtggcg
     2581 ctgcgccaga tgatcaatcc aggggcgctg atcatcggct ccgactcgat cgaaaccctg
     2641 gcccggcggc gtctcaccac ctacgagctg cgccagtgcg gcccgttcgg cacctggcgc
     2701 aacccctcgc gcaactcgtg gcatgtcgtg ttcgactacc gccaagacag cgacgacgac
     2761 ccgatccatc gcgccaaccg ccaactcatg ggtggctcgc gagtgctgtt cgagcactac
     2821 ggcgtacccg cggaagaact ggccgagatg ctcgaacgag cccgccagcg ggcactgacc
     2881 gcggacgcct gagacccggc gctcggccag tcaggaccga gggtcgctca ggggatgaac
     2941 agctcgcatt gagcgcggcg cccttgcgca ggttgtcgtt gctgaggaac agggccaggc
     3001 cacggccgtc ggcgacgcct tcgtcgacgc ggatacgacc gacgtagctc gcgtcacgcc
     3061 cggccgcttc gagtggcgtc ggcacgtcgg tcacgatcac accgggcgct gcggcgagca
     3121 actcgatggc ccgagcgacg ctgagcgggc gggcgaactc ggcgttgatg ctgaggctgt
     3181 gaccggtgaa gaccggcacg cgcacacagg taccgctgac cgccagaccc gggatgtcga
     3241 gaatcttgcg cgtctcgttg cgcagcttct gttcttcgtc ggtctcgaac aggccgtcat
     3301 cgaccagctt gcccgcgtac ggcaacacgt tgaacgcgat cggcttggcg aacttggccg
     3361 gcgcaggcat cgacacggca gcaccgtcgt aggtgagctc gcgggcggtg tcgaccacct
     3421 cgcgcacctg cttgtcgagc tcgtcgaccc cggccaggcc gccgccgctg accgcctgat
     3481 aggtggccgc gatgagacgc accagtccgg cctcgtcgtg cagcggcttg agcaccggca
     3541 tcgccgccat cgtggtgcag ttggggttgg cgatgatgcc cttcggcgcc tggcgggcga
     3601 gatgtccgtt gacctcgctg acgatcagcg gaacgtcggg gtccatccgg aacgccgacg
     3661 agttgtcgat gacggtcacg ccggccgcgg cgaacttggg ggcgagctct ttggacgagg
     3721 tggccccggc ggagaacagc gcgatgtcga gcccggtggg atcagcgatg gcagcgtctt
     3781 cgacggtgat ctctccgtcg agccacggca gcgtgatgcc cgccgatcgc gccgaggcga
     3841 agtagcgcaa ctcggtgacg gggaactttc gctcggcgag gatggcgcgc atgacgccgc
     3901 cgacttgacc agttgctccg acgatcccaa ctctcatggt gcatcaggct atggccccga
     3961 tccctggacc catcagggaa tatgcatgcc aacgggggtt acgacttggt ggcgacgagc
     4021 caccagtggc tgcggttgac accgacgaag gtgcgcagcg tcgcgtcgtc acgcatgccg
     4081 attcggcgat gcggtgagtg gaagatgacc cgatcggcca tctgaccggg cagggtcgcc
     4141 aggtgttgcg acacctgctc gggcgacagc cg
//
LOCUS       DAORNF010000240         4163 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_15978_length_4163_cov_1.821811, whole genome shotgun sequence.
ACCESSION   DAORNF010000240 DAORNF010000000
VERSION     DAORNF010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4163
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15978_length_4163_cov_1.821811"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..695)
                     /locus_tag="PLV68_10320"
     CDS             complement(<1..695)
                     /locus_tag="PLV68_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HRE01685.1"
                     /translation="MAEPADHLARIESLREQIAYHNHRYNDLDDPEISDADYDELMRE
                     LRALEELHPDTLSPDSPTQTVGGAVSAAFAPVLHRVPMTSLDNAMDAGELRAWGDRVV
                     KGLAGQVPTFVCELKIDGVAMSLRYEAGRLVQAATRGDGRVGEDVTANIKTIAVIPHQ
                     LSGAAAIDVLEVRGEVYMPRASFESFNDAAMAAGQKLLVNPRNAAAGSLRQKDSSITA
                     QRSLAFWCYQLGEV"
     gene            complement(732..>1184)
                     /locus_tag="PLV68_10325"
     CDS             complement(732..>1184)
                     /locus_tag="PLV68_10325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01686.1"
                     /translation="AVDLLSSALAVIEPHATAGVHSCGDADWATVMAAGPTLLSMPAL
                     PTLLNSVGYIQRFLDRGGWVAWGAVPTDGPVSMSAERPWKQLCDLWCGMTQRGCDTAM
                     LRQQSLVTPACGLALHRPVMAERLLRNTAVIAGRVRDQAAATRFAFGA"
     assembly_gap    1185..1251
                     /estimated_length=67
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1252..1774)
                     /locus_tag="PLV68_10330"
     CDS             complement(<1252..1774)
                     /locus_tag="PLV68_10330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01687.1"
                     /translation="MTTSDNTSARPAASAPVMPGRISGGMSMAIGSLPYLDVGDAVAA
                     ALAATDIVTIPTTPRRSPAEGMLAQALIGIQGVSISALGTILVDPLKVTAGAAVTTDL
                     GHDAFATMRAFLDAGPQFSGPVKWQFVGPVTLGLALQRAGVRASVAFEVAAHAVRSHV
                     EHLLTAVAAALPGS"
     assembly_gap    2157..2374
                     /estimated_length=218
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2375..>2659
                     /locus_tag="PLV68_10335"
     CDS             <2375..>2659
                     /locus_tag="PLV68_10335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01688.1"
                     /translation="SSLAVAAWFSGDVGAVFAQGSAYGAEQRFPLKAPVSYQLPMALT
                     WVVTAGLALTGATLLANEVWIVGVAVLGALGQEGRDVVEEFLIGRVGELLG"
     assembly_gap    2660..2795
                     /estimated_length=136
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2988..4046)
                     /gene="mnmA"
                     /locus_tag="PLV68_10340"
     CDS             complement(2988..4046)
                     /gene="mnmA"
                     /locus_tag="PLV68_10340"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HRE01689.1"
                     /translation="MKVMVAMSGGVDSSVAAALLVEAGHEVVGVTMRLWGGESDTGCC
                     SVSDVDDARRVAQQLGIDHLVFNFTDDFDHHVVQPYVHAHAVGVTPNPCIECNRHVKF
                     RRLSERAELLGFDAVATGHHARIGQSGAGRELERGADRAKDQSYVVHMISRDELDRTL
                     FPVGSMTKTDVRAHAERLGLRTAAKPDSQDVCFITSTGGRTAFLGARIPFRPGLVVDT
                     EGSQVGTVPAVEMVTLGQRRGIGLPGGGPKQYVVGVDVATATVTVGPERDLFVGGESV
                     GEMTWLHDPASVAGCEVLVQCSAHGTPQPATVELTDAAGASIRLTWNDPQRRIAPGQS
                     IVLYDQTDRFVLAGGTCL"
BASE COUNT          577 a         1301 c         1292 g          572 t
ORIGIN      
        1 acctcgccca gctggtagca ccagaacgcg agcgaacgtt gggcggtgat cgaagagtcc
       61 ttctggcgca ggctgcccgc cgcggcgttg cgcgggttga ccagcagctt ctgcccggca
      121 gccatcgccg cgtcgttgaa cgactcgaag ctggcccgcg gcatgtatac ctcgccacga
      181 acctccaaca cgtcgatcgc cgcggcaccg ctgagctgat gcgggatgac ggcgatggtc
      241 ttgatgttgg cggtgacgtc ttcacccaca cggccgtcgc cgcgggtggc cgcctgcacg
      301 agtcggccgg cctcgtagcg caagctcatc gccactccgt cgatcttcag ctcgcacacg
      361 aacgtgggca cctgcccggc caagcccttg accacccggt cgccccaggc gcgcagttca
      421 ccggcgtcca tggcgttgtc gagactggtc atcggtaccc ggtgcaacac cggcgcaaag
      481 gccgccgaca ccgctccgcc gacggtctgg gttggcgaat caggcgacaa cgtgtcgggg
      541 tgcagctcct ccaacgcgcg cagctcgcgc atcaactcgt cgtagtcggc gtcgctgatc
      601 tcgggatcgt cgaggtcgtt gtagcggtgg ttgtggtacg cgatctgctc gcgcagcgac
      661 tcgatgcggg ccaggtggtc ggcgggttct gccacgccgt gcaccctacc cagggcctgc
      721 gcgccgatcc gtcaagcgcc gaaggcgaag cgggtggcag cggcctggtc gcggacgcgt
      781 ccggcgatga ccgccgtgtt gcgcaacagc cgctcggcca tgaccgggcg atgcagtgcc
      841 aagccgcacg ctggggtcac cagactttgc tgacgcagca tcgcggtatc gcagccacgc
      901 tgggtcatgc cgcaccacag gtcgcagagt tgcttccacg gccgctcggc cgacatcgac
      961 accggcccgt cggtaggcac cgcgccccat gcgacccaac cgccccggtc gaggaagcgc
     1021 tggatgtagc cgacgctgtt gagcagcgtc ggcaacgcag gcatcgacag caacgtcggc
     1081 ccggcggcca tcaccgtggc ccagtcggcg tcgccgcagc tgtgcacgcc tgccgtggcg
     1141 tgcggctcga tgacggcaag ggcgctcgac agcaggtcga ccgcnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncgctacccg
     1261 gcaacgccgc ggcgactgcc gtgagcaggt gctcgacgtg cgatcgcacc gcgtgagcgg
     1321 caacctcgaa ggccaccgag gctcggaccc cggcccgctg caaagccaac cccaacgtga
     1381 ccgggccgac gaactgccac ttgaccggtc cggagaactg tgggccggcg tcgagaaacg
     1441 cgcgcatcgt ggcgaacgca tcatgaccga ggtcggtcgt gaccgcagcg ccggcggtga
     1501 ccttgagcgg gtcgaccaga atcgtgccga gggcgctgat cgatacgccc tggatgccga
     1561 tcagtgcctg ggcgagcatg ccctcagccg gtgaacggcg gggggtggtg gggatggtga
     1621 cgatgtcggt cgccgcgagc gcggcggcga cggcgtcgcc gacgtcgagg tacggcaggc
     1681 tgccgatcgc catcgacatg cctccactga tgcgccctgg catgaccggg gccgacgcgg
     1741 cagggcgagc actggtgttg tccgatgtgg tcatctgcgg tgctcccttc tgagtcaggc
     1801 ttggcggtca ggtcatcggc gctcggccgt ggtggatcgg gtgccggggg tcggcgagga
     1861 cgtgcggcac aaggccgcgg cgagtggggt gtgtggcgcc gacgagcgga ctttcggccg
     1921 ttcggcgagc accgttatcg tgacacaggc ggtcgcccgg tccacaaccg gccaccaccg
     1981 aatccttgcg aatcagccat gaacgacccg accgccagcg gcatcgaccg caccaccgcc
     2041 aacagtgccc ccggcagcac cgcgcgccaa cccgggtcac gcagcgggat catcgcattg
     2101 tgggtgctgc gggtcgtatg gctggtacag ccgttggcct tcgtgccggc gctgggnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnatcgtc actcgccgtc gcggcgtggt
     2401 tcagcggcga tgtcggggcg gtgttcgcgc aggggtcggc ctacggcgcc gagcagcgct
     2461 ttcctctcaa ggctccggtc tcgtatcagt tgccgatggc gctcacctgg gtcgtcaccg
     2521 cgggcctggc cctcactggc gccaccttgc tcgccaacga ggtgtggatc gtgggcgtcg
     2581 ccgtccttgg cgcccttggt caggaaggcc gcgacgtcgt cgaggagttc ctgatcgggc
     2641 gtgtcggcga acttctcggn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnaccgc cggtgtcgcg gtcgagatcg
     2821 agctgcgcga catggagacc atcgtccgcg tcgaccgcac caaaccgcaa ggcgccgcgg
     2881 tccacgtccg cagcctgctg gtcgcgccca ctcggcccgg caaagctctc acggctgccg
     2941 cggcaaccca cctccccgtc cgttagatcg ctgcaccgcg ccaggggtca gaggcaggtg
     3001 ccgccggcca acacgaagcg gtcggtctgg tcgtacagga cgatgctctg gccgggagca
     3061 atgcgccgct gcgggtcgtt ccatgtgaga cgaatcgacg cgccggctgc gtcggtcaac
     3121 tcgacggtgg cgggttgggg cgtgccgtgg gcgctgcact gcaccagcac ctcgcagccc
     3181 gcaaccgagg ccggatcgtg caaccaggtc atctcgccga ccgactcgcc cccgacgaac
     3241 aggtcgcgct ccggtccgac cgtgacggtg gccgtcgcga cgtcgacgcc gaccacgtat
     3301 tgcttcggtc caccgccggg caagccgatg ccccgtcgct ggcccagcgt gaccatctcc
     3361 accgcgggaa cggtgccgac ctgcgacccc tccgtgtcga ccacgaggcc aggtcgaaac
     3421 gggatccgcg cgcccagaaa cgcggtacga ccgccggtcg aggtgatgaa gcagacgtcc
     3481 tggctgtcgg gcttggcagc ggtacgcaaa ccgagccgct cggcgtgagc gcgcacgtcg
     3541 gtcttggtca tcgaacccac cggaaacagc gtgcggtcga gctcgtcacg gctgatcatg
     3601 tgcacgacgt agctctggtc cttggcccgg tcggcgccgc gttcgagctc gcggcccgcg
     3661 cccgactggc cgatgcgggc gtgatggccg gtggcgaccg cgtcgaagcc gagcagttcg
     3721 gcccgctcgc tgaggcggcg gaacttgacg tgacggttgc actcgatgca cgggttggga
     3781 gtgacaccaa ccgcatgggc gtgcacgtag ggctgcacca cgtggtggtc gaagtcgtcg
     3841 gtgaagttga acaccaggtg gtcgatgccc aactgctggg ccacccggcg ggcatcgtcc
     3901 acatcgctga cgctgcaaca gccggtatcg ctctcgccgc cccacagccg catcgtgacg
     3961 ccgaccacct catggcccgc ttcgaccaac agcgcggccg cgaccgacga atcgacaccg
     4021 ccgctcatcg cgaccatcac cttcatcgcg ggaccttcac gctgggacgc ccctcatacc
     4081 cgagccatca cggctgcggg tgcgcagcgt cgcgatcgca acggtgatga cctggactgc
     4141 ccggtcgacc tgggcgtcgg tcg
//
LOCUS       DAORNF010000241         4163 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_15980_length_4163_cov_1.674051, whole genome shotgun sequence.
ACCESSION   DAORNF010000241 DAORNF010000000
VERSION     DAORNF010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4163
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15980_length_4163_cov_1.674051"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    220..282
                     /estimated_length=63
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<283..1196)
                     /locus_tag="PLV68_10345"
     CDS             complement(<283..1196)
                     /locus_tag="PLV68_10345"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRE01690.1"
                     /translation="MSEADLGHTGEAPLLEVRDATVRFATRTGSLLAVDRVSFDIGRG
                     ETLGIVGESGSGKSVLVRGLMGLLPRSATIGTGTSIRFDGSELVHDGRVEAASLWGRR
                     IAMIFQDPMTSLNPVKRIGVQLTEVMRAHLGYDLQRAKQRATELLDEVGISDPGRRLR
                     QYPHEMSGGMRQRIMIAMALICDPELLIADEPTTALDVTVQRQVLELLEEVQQRHHMA
                     VIFISHDLGVVSEIADRVAVMYAGQFVEQAAADDLFTRVTHPYTEALLQSMPRVDGRA
                     GVRLSPIPGQLPNMTSPPFGCRFAERCR"
     gene            complement(1218..>1412)
                     /locus_tag="PLV68_10350"
     CDS             complement(1218..>1412)
                     /locus_tag="PLV68_10350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01691.1"
                     /translation="QLREAATADERNAAVAAVQAVWTEQVPALPLTVFEPSLLWRDGV
                     EGIYFNTNLLAFLDTAYLAS"
     assembly_gap    1413..1449
                     /estimated_length=37
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1450..2559)
                     /locus_tag="PLV68_10355"
     CDS             complement(<1450..2559)
                     /locus_tag="PLV68_10355"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRE01692.1"
                     /translation="MPQPASSCRTWPNADNTVWTLTLREGVTFGNGDPVDAAAVKASF
                     DRHLDPEGGSALAGNAAQIAGVEVAGDLAVEFTLRDPWAGFPSLLAGAFGLVQNVAVI
                     DAIGQEAFASNPVGGGAGPFEVSNFSAGQSITLEAKDDYWGGDVCLDKIVFTIIPQNR
                     TAYDALRTGEVDVMLLAQDPELLAEYREDAGSFESFGAAQIGGDLLLINSGADGADTP
                     GADVRVRQAIAAAIDPQLINDRAFSGSALMSTGLIPAGSEFFTPTTGPAYDPDAAAAL
                     VAEVKADTGWDGTIRLASANTSAKSEAAIAVKAMLDAVGFNVDLSSQHTGTEMSTLVR
                     VDRDFDLAMWGLSAHEENLWTTLKQYYSDAPNNAGN"
     gene            2631..2942
                     /locus_tag="PLV68_10360"
     CDS             2631..2942
                     /locus_tag="PLV68_10360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01693.1"
                     /translation="MNGSNVREPAKSETFNVPPVAGHRSAGCSPVVGVLEPLVVGVLE
                     PSVVGVAELPVVEPVVGAVELLVGPASLASDPQPAVSANTPANVALQNNRTLMVSFPL
                     L"
     gene            complement(3189..>3449)
                     /locus_tag="PLV68_10365"
     CDS             complement(3189..>3449)
                     /locus_tag="PLV68_10365"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRE01694.1"
                     /translation="KMVPGDLGTKGGLLTDELARVLDNSGTPIEGLYAVGNAAASVMG
                     REYPGGGSTIGPGLTFGYVAAVDVAARLAATTDAESGNQARA"
     assembly_gap    3450..3552
                     /estimated_length=103
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3553..>4163)
                     /locus_tag="PLV68_10370"
     CDS             complement(<3553..>4163)
                     /locus_tag="PLV68_10370"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRE01695.1"
                     /translation="VGALVDRDGVPTRVRVRGGVVIASGGFERNPAMRDQYQPKPITT
                     TWTMGVAENTGDGIRAGAAVGGALDLMDEAWWGPSLLTPEGKGAFTERAEPRSMLVNG
                     NGERYTNEAAPYVNVCHAMYRGEATGVTHVPSWFVVDELYRRRYKLGPFLPRQPIPAA
                     WFESGVAVKAQTIPELAAKIGVPADRLVASVARMNELARIGKD"
BASE COUNT          666 a         1314 c         1300 g          680 t
ORIGIN      
        1 gacgacgctg agcgtctcgc cttcggccag gtcgaagctc agacccgaca cgaccttgac
       61 cggcttgcgg ttcgggccgg gataacagac atcgagatcc tcgacgcgca ccaacacgtc
      121 gaggtggtcg cgcagttgaa cacttcccag gccgaccatc aggccacctt catcggatcg
      181 aagtcgaccg ggtgcacaca acggcacaag cgatcgtcgn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntagcgaca ccgctctgcg
      301 aatcggcatc cgaacggagg actggtcatg ttcggaagct ggcccgggat cggtgacagg
      361 cgaactccgg cgcgaccgtc gactcgaggc atgctctgca acaacgcctc ggtatacgga
      421 tgggtcaccc gggtgaacag atcgtccgca gcggcctgct cgacgaactg gcccgcgtac
      481 atcaccgcga cgcggtcggc gatctcggag accacgccga ggtcatggct gatgaagatc
      541 accgccatgt gatggcgctg ctgcacctcc tcgagcagtt cgagcacttg ccgctgcacg
      601 gtgacgtcga gtgccgtggt cggttcgtcg gcgatcaaca actccggatc gcagatcaac
      661 gccatcgcga tcatgatccg ctggcgcatc ccacctgaca tttcgtgcgg atactgccgc
      721 aaccgacgac ccggatcgga gatcccgacc tcgtcgagca gttcggtcgc acgctgtttg
      781 gcccgctgca ggtcgtagcc gagatgggca cgcatcactt cggtgagctg caccccgatc
      841 cgcttgacgg gattgagcga cgtcatcgga tcctggaaga tcatcgcgat ccgacggccc
      901 catagcgatg ccgcctcgac acggccgtcg tgcaccagtt cggagccgtc gaagcggatc
      961 gaggtgccgg tcccgatcgt ggccgagcga ggcagcagcc ccatcaaccc acggacgagc
     1021 acggacttgc cggaccccga ctcaccgacg atgcccaagg tctcaccccg accgatgtcg
     1081 aagctgaccc ggtccacggc gagcagagat cccgtcctgg tggcgaaacg cacagtggcg
     1141 tcacgaacct ccaggagcgg tgcttcgccc gtatggccca ggtcagcttc ggacacgatc
     1201 atggctcgat ctggcactca gctcgcgagg taggcggtgt cgaggaaggc caggagattg
     1261 gtgttgaagt agatgccctc gacgccgtct cgccacagca agctgggctc gaagaccgtc
     1321 aacggaaggg caggaacctg ctcggtccac accgcttgga ccgcggccac cgcggcgttg
     1381 cgctcgtcag ccgttgcggc ctcacggagc tgnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnng ttgccagcgt tgttgggagc gtcggagtag tactgcttga gcgtggtcca
     1501 caggttctcc tcgtgggccg acagacccca catcgccaga tcgaagtcgc gatcgacacg
     1561 caccagcgtg gacatctcgg tccctgtgtg ctggctcgac agatcgacgt tgaagccgac
     1621 agcgtcgagc atcgccttga ccgcgatggc ggcttcggac ttggccgacg tgttcgccga
     1681 agcgaggcgg atcgtaccgt cccatcccgt atcggccttg acctctgcga ccaacgctgc
     1741 ggcagcatcg gggtcatagg ccggacctgt cgtcggggtg aagaactcag accccgcggg
     1801 gatcaatccg gtgctcatca aggccgaacc gctgaaggcg cgatcgttga tgagctgcgg
     1861 gtcgatcgcc gcagcgatcg cctggcgaac acggacgtcg gcacccgggg tgtcggcacc
     1921 gtctgctccg gagttgatca acagcaggtc gccaccgatc tgggccgcgc cgaagctctc
     1981 gaagctgccg gcgtcctcgc ggtattctgc gagcaactcc ggatcctggg cgagcaacat
     2041 cacgtcgacc tccccggtgc ggagcgcgtc gtacgccgtc cggttttggg gaatgatggt
     2101 gaagacgatc ttgtcgaggc agacgtcgcc accccaatag tcgtccttgg cctcgagggt
     2161 gatcgactga cccgccgaga agttggagac ttcgaacggg ccggcgccgc ccccgactgg
     2221 gttggacgcg aacgcctctt gaccgatcgc gtcgatgacg gccacgttct ggaccaggcc
     2281 gaaggccccg gcgagcagcg acgggaagcc cgcccacgga tcgcgcagcg tgaactccac
     2341 cgcgaggtcg ccggccactt cgaccccggc gatctgggcc gcgtttccgg cgagggccga
     2401 accgccctcg ggatcgaggt gacggtcgaa ggaggccttc accgcggcag cgtcgaccgg
     2461 gtcgccgttg ccgaaggtga cgccctcgcg caacgtcagt gtccagacgg tgttgtccgc
     2521 gttcggccat gtgcggcacg aactcgccgg ttgcggtatc gaaccggatg agcttgtcgt
     2581 agatcgcctg cagttcaatg ccgccctgct ctgatgtgtt gccaccgccg ttgaacggat
     2641 ccaacgtccg cgagccggcc aagagcgaga cgttcaacgt cccgccggtc gccgggcacc
     2701 gctcggccgg ttgctcgccg gtggtcggcg tgctcgagcc gctcgtggtc ggtgtgctcg
     2761 aaccgtcggt cgtcggtgtg gccgaactcc cggtcgtcga gccggtggtc ggcgcggtcg
     2821 agttgctcgt cggtccggcc tctttggcgt cagatccgca gcctgcggtc agtgccaaca
     2881 cccccgccaa cgttgcgctc caaaacaatc gaactctcat ggtgtctttc cccctcttgt
     2941 gagtcagatg agcatcgatt tcagagccgg ttctggcaac ggttgccgtc cggtggatgg
     3001 gacagccgac acggacatca tggccgtggc tcggggagcg acacaaccag gtccgtgccc
     3061 acacgccaag caccgccacg acttgctgtc gtggcggtgc ttgcttgttc tcgtgcttgg
     3121 tgattcggag cggtcgtggg acgcgacccg gtccggccca gatcgttcga atcggccggt
     3181 tggcggtgtc aggcgcgggc ttggttgccg gattcggcat ccgtggtggc agcgagccgg
     3241 gcagccacat cgacggccgc gacgtagcca aaggtgaggc cggggccgat ggtcgatccg
     3301 ccgccggggt attcgcgtcc catcaccgac gccgctgcgt tccctactgc gtacagacct
     3361 tcgatgggtg tgccgctgtt gtcgagcacg cgggccaatt catcggtcag caggccaccc
     3421 ttggtgccga ggtcgcccgg caccatcttn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nngatccttg ccaatgcgag ccagttcgtt catccgcgcg accgatgcga
     3601 caaggcggtc tgcgggaacg ccgatcttgg ccgccaactc ggggatggtt tgggccttga
     3661 cggccacgcc gctctcgaac caggccgccg ggatcggctg acggggcaag aacggaccga
     3721 gcttgtaccg gcggcggtac agctcgtcga ccacgaacca cgacggcaca tgggtcacgc
     3781 cggttgcctc gccccggtac atggcatggc agacgttcac gtagggggcc gcttcgttgg
     3841 tgtagcgctc gccgttgccg ttgaccagca tcgaacgggg ctcggcacgc tcggtgaagg
     3901 ctcctttgcc ctcaggggtc agcagcgacg gcccccacca cgcctcgtcc atcaggtcga
     3961 gggcgcctcc gacagccgcg ccggcacgga tgccgtcgcc ggtgttctcg gcgacaccca
     4021 tcgtccacgt cgtcgtgatc ggcttgggct gatactggtc gcgcatcgcc gggttgcgtt
     4081 cgaatcctcc actcgcgatg accacgccac cgcggacgcg gacacgagtc ggcacgccgt
     4141 cgcggtcgac gagcgcgccg acc
//
LOCUS       DAORNF010000242         4162 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_15989_length_4162_cov_1.488921, whole genome shotgun sequence.
ACCESSION   DAORNF010000242 DAORNF010000000
VERSION     DAORNF010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4162
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15989_length_4162_cov_1.488921"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>259)
                     /locus_tag="PLV68_10375"
     CDS             complement(<1..>259)
                     /locus_tag="PLV68_10375"
                     /inference="COORDINATES: protein motif:HMM:NF013784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HRE01696.1"
                     /translation="KTGRDVVIAALLGAEEFGFATAPLVVSGCVMMRVCHLDTCPVGV
                     ATQNPELRKRFSGKPEFVVNFFEFIAEEVRELMAGLGFRTMA"
     assembly_gap    260..363
                     /estimated_length=104
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<364..>638)
                     /locus_tag="PLV68_10380"
     CDS             complement(<364..>638)
                     /locus_tag="PLV68_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HRE01697.1"
                     /translation="GHKVYPWVAKTRHSTPGVGLISPPPHHDIYSIEDLKQLIHDLKN
                     ANPAARVHVKLVAEMGVGTVAAGVAKAKADVVLISGHDGGTGASPLTS"
     assembly_gap    639..724
                     /estimated_length=86
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<725..>1070)
                     /locus_tag="PLV68_10385"
     CDS             complement(<725..>1070)
                     /locus_tag="PLV68_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HRE01698.1"
                     /translation="QHATRAKRYDIFKQYTRLVDDQSQRLATLRGLFNLHSHRTPVPI
                     DEVEPVSEIVKRFSTGAMSYGSISAEAHETLAIAMNSIGAKSNTGEGGEDSDRLVDPR
                     RRSAIKQVASGRF"
     assembly_gap    1071..1085
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1086..>2596)
                     /locus_tag="PLV68_10390"
     CDS             complement(<1086..>2596)
                     /locus_tag="PLV68_10390"
                     /inference="COORDINATES: protein motif:HMM:NF016768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate synthase central domain-containing
                     protein"
                     /protein_id="HRE01699.1"
                     /translation="LMMIPEAWENNTAMDPKRRAFYRFHASLMEPWDGPASVAFTDGT
                     VIGSVLDRNGLRPGRYWVTDDDIVVLASEAGVIGVDPAKVVQKGRLQPGRMFLVDTAA
                     GRIVSNDEIKDLLAAEHPYGEWLESGLIQLDDLPAREHVVFSHSSVLRRQQVFGYTHE
                     ELKIILAPMAKSGAEPIGSMGTDTPIAALSQRSRLLFDYFSQLFAQVTNPPLDAIREE
                     VVTSVGTAVGPEHNLLAPGPESCRQLTLPFPIIDNDELAKIIKVNEDGDFPHLKAVTL
                     SGLYRVNGGGLALKRALDMIRSEASKAIDDGARIIVLSDRNSDEALAPIPSLLLTAAV
                     HHHLVREQKRTQVGLIVECGDAREVHHMALLIGYGAGAINPYLAFESIEDMIAEGLNG
                     LGGMDPHVAIKHFIKASGKGVLKVMSKMGVSTVASYTGAQIFEAIGLGRELVDEYFTG
                     TVSRLGGMGLDGVAAEVAARHAMAHPTRPEERAHRKLELGGEYQWRREGELHL"
     assembly_gap    2597..2608
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2609..>2828)
                     /locus_tag="PLV68_10395"
     CDS             complement(<2609..>2828)
                     /locus_tag="PLV68_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01700.1"
                     /translation="PSWPLAHPYRYIAHNGEINTVQGNRNWMRTREALVHSDLLPGVE
                     RAFPICTPGGSDSASFDEVLELLHLGGRS"
     assembly_gap    2829..2949
                     /estimated_length=121
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2950..3525)
                     /locus_tag="PLV68_10400"
     CDS             complement(<2950..3525)
                     /locus_tag="PLV68_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01701.1"
                     /translation="MFNDHPAPVGLYDPRFEHDACGVGFVAHIKGVASRSIVDLALGA
                     LCNMEHRGATGAEVDTGDGAGVLIQVPDAFLRGVVPFALPPAGHYAVGLAMLPAAPED
                     AEKAIASIEAIVADEGLRVLGWRDVPIEPACLGATALTVMPSFRHLFIAGEGPAAHAD
                     LAGIELDRRRGLDHGAWSPLRFMYPAADVPVI"
     assembly_gap    3779..3911
                     /estimated_length=133
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3969..>4162
                     /locus_tag="PLV68_10405"
     CDS             3969..>4162
                     /locus_tag="PLV68_10405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01702.1"
                     /translation="MTVGQTIEISIVNDTDQDSEFTHFNFFYSGKIPRYIVNLNLDSP
                     GIRELEPGTPLTVPPVAPVRL"
BASE COUNT          580 a         1257 c         1226 g          628 t
ORIGIN      
        1 ccgccatcgt gcggaagccg agcccggcca tgagctcgcg cacctcttcg gcgatgaact
       61 cgaagaagtt gacgacgaac tcgggcttgc cactgaaccg cttgcgcagc tcgggatttt
      121 gggtggcgac gccgaccgga caggtgtcca ggtggcagac acgcatcatc acgcatccac
      181 tgaccaccag cggcgcggtc gcgaacccga actcctcggc accgagcagc gccgcgatca
      241 ccacgtcgcg accggtcttn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnngacgtca gcggcgaggc accggtgccg ccgtcgtggc cgctgatcag caccacgtca
      421 gccttggcct tggccacacc ggccgccacc gtaccgacac ccatctcggc caccagcttg
      481 acgtggacac gtgccgccgg gttggcgttc ttgaggtcgt ggatgagctg cttcaggtcc
      541 tcgatcgaat agatgtcgtg gtgcggcggg gggctgatca agccgacgcc cggcgtgctg
      601 tggcgggtct tggccaccca cggatacacc ttgtggccnn nnnnnnnnnn nnnnnnnnnn
      661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      721 nnnncgaagc gcccgctggc gacctgcttg atcgcactgc gccgccgcgg gtcgaccaga
      781 cgatcggagt cctcgccgcc ttcgccggta ttgctcttgg ccccgatcga gttcatcgcg
      841 atcgccagcg tctcgtgcgc ttcggcgctg atgctgccgt agctcatcgc cccggtggag
      901 aaccgcttga cgatctcgct caccggctcg acctcgtcga tcggcaccgg ggtgcggtgg
      961 ctgtgcaggt tgaacaggcc gcgcagagtt gccaggcgtt ggctctggtc gtcgaccagg
     1021 cgggtgtact gcttgaagat gtcgtagcgc ttggcccgcg tggcgtgctg nnnnnnnnnn
     1081 nnnnnacagg tgcagttcac cctcacgtcg ccactggtac tcgccgccga gctcgagctt
     1141 gcggtgcgct cgttcctccg gccgtgtcgg atgagccatc gcgtgccgag cggcgacttc
     1201 ggccgcgacg ccatcgaggc ccatgccgcc caagcgactg accgtgccgg tgaagtactc
     1261 gtccaccaac tcgcgtccga gcccgatcgc ttcgaagatc tgtgccccgg tgtacgacgc
     1321 cacggtgctc acgcccatct tcgacatcac cttcagcacg cccttgccgg acgccttgat
     1381 gaagtgcttg atcgcaacgt gcgggtccat cccaccgagg ccgttgaggc cctcggcgat
     1441 catgtcctcg atcgattcga acgccaggta ggggttgatc gccccggcgc cgtagccgat
     1501 cagcagggcc atgtggtgta cctcgcgggc gtcgccgcac tcgacgatca ggccgacctg
     1561 cgtgcgcttc tgctcgcgca ccaggtggtg gtgcaccgcc gcggtcagca gcagcgatgg
     1621 gatcggagcg agcgcttcgt cgctgttgcg gtcgctgagc acgatgatcc gcgccccgtc
     1681 gtcgatcgcc ttgctcgctt cggaacggat catgtcgagc gcccgcttca gcgccagacc
     1741 gccgccgttg acccggtaca ggccgctgag cgtgaccgcc ttaaggtgcg ggaagtcacc
     1801 gtcctcgttg accttgatga tcttggccaa ctcgtcgttg tcgatgatcg ggaacggcag
     1861 cgtcagttgg cggcagctct ctgggccggg ggcgagcagg ttgtgctcgg gaccgaccgc
     1921 ggtgcccacc gaggtgacga cctcttcccg gatcgcatcg agcggcgggt tggtgacctg
     1981 ggcgaacagc tggctgaagt agtcgaacag cagccgtgag cgctgactga gcgcggcgat
     2041 cggtgtgtcg gtgcccatcg atccgatcgg ctcggcaccg ctcttggcca tcggggcgag
     2101 gatgatcttc agctcttcgt gggtgtagcc gaacacctgc tggcggcgca gcacgctgct
     2161 gtggctgaac acgacgtgct cgcgggcggg caggtcgtcc agctggatca ggcccgactc
     2221 cagccattct ccgtagggat gctcggccgc gagcaggtcc ttgatctcgt cgttcgacac
     2281 gatccgtccc gccgcggtgt cgaccaggaa cattcggcct ggctggaggc ggcccttctg
     2341 gacgaccttg gccgggtcga cgccgatcac gccggcctcg ctggccaaca ccacgatgtc
     2401 gtcgtcggtc acccagtagc gcccggggcg cagcccgttg cggtcgagca ccgagccgat
     2461 cacggtgccg tcggtgaacg cgacgctggc agggccgtcc cacggctcca tcagcgacgc
     2521 atggaagcgg tagaacgcgc gccgcttggg gtccatcgcg gtgttgttct cccacgcctc
     2581 ggggatcatc atcaacnnnn nnnnnnnnga gcgtccgccc aggtggagca gttccagcac
     2641 ctcgtcgaag ctggccgagt ccgacccgcc gggcgtgcag atcgggaacg cccgctcgac
     2701 accgggcagc agatcgctgt gcaccaacgc ctcgcgggtc cgcatccagt tgcggttgcc
     2761 ctgcacggtg ttgatctctc cgttgtgggc gatgtagcgg tacgggtggg ccagcggcca
     2821 cgacgggann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnng atcaccggaa cgtcggccgc cgggtacatg aagcgcagcg gcgaccaggc
     3001 accgtgatcg aggccgcgcc gccggtcgag ctcgatgccc gccaggtcgg cgtgggccgc
     3061 gggcccctct ccggcgatga acaggtggcg gaacgacggc atcacggtca gcgcggtcgc
     3121 accgagacag gccggttcga tcggtacgtc gcgccagccg agcactcgca gaccctcgtc
     3181 ggcgacgatc gcttcgatcg aggcgatcgc cttttcggca tcctcgggcg cggcgggcag
     3241 catcgccagg ccgaccgcat agtgcccggc aggtggcagc gcgaacggca cgacgccacg
     3301 caggaacgca tcgggcacct ggatcagcac gcctgcgccg tcaccggtgt cgacctcggc
     3361 gccggtggcc ccgcggtgct ccatgttgca cagcgcgccg agtgccaggt cgacgatcga
     3421 gcggctggcg acgcccttga tgtgggcgac gaagccgacg ccacaggcgt cgtgctcgaa
     3481 gcgggggtcg tagagaccca cgggcgcggg gtgatcgttg aacatcgtcc tcatccactg
     3541 aagtcgtagg gcttggtgga gtccgggacg acgctggccc aaacagtgcc ccgatgttac
     3601 gcccctgttt cgtccaggcc aactctttcc acgcccgacc caggtgcccc aaccaccagg
     3661 ctgcgctcgg cacgtccgtg ggcaggcgca gcaatgctcg tcacgtcgag cgaacaggcg
     3721 tgtgccgatg gtcgagccgt ctgtcgggca ccggcggcgg gaggccgcga tctgacccnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnnnnnn ncgcagccac aactcgaccg aggcaccagc catccatctc gtcaacacgg
     3961 ttcgctgcgt gaccgtcgga cagacgatcg aaatctcgat cgtcaacgac acggaccaag
     4021 actccgagtt cacgcacttc aacttcttct acagcgggaa gatccctcgg tacatcgtga
     4081 acctgaacct ggacagtcca ggtatccggg aactcgaacc gggaactccg ctgaccgttc
     4141 cgccagtcgc tcctgtgcgg ct
//
LOCUS       DAORNF010000243         4161 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_15994_length_4161_cov_1.759377, whole genome shotgun sequence.
ACCESSION   DAORNF010000243 DAORNF010000000
VERSION     DAORNF010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4161
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15994_length_4161_cov_1.759377"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="PLV68_10410"
     CDS             complement(<1..530)
                     /locus_tag="PLV68_10410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01703.1"
                     /translation="MGLLRLRVVPTRLRPIDVTSAAITVAAVTVAVSQRSRRARWRLI
                     AIAQSFTPMLGVAAAANALAAVLRRRRVTAIVNTCAALALAAVVAPALTTKRPRRWGD
                     DDPRPGESETGLRVAHANLLYQATDDARAAIGAVLATDADVLALTEFTLEHEAALAAL
                     DTDHRYRYCCGQAVSGA"
     gene            complement(532..2952)
                     /locus_tag="PLV68_10415"
     CDS             complement(532..2952)
                     /locus_tag="PLV68_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HRE01704.1"
                     /translation="MKAHATPTELLQHRFPVDPWRLVEREYSTADLGVTETLFATANG
                     YIGMRANPDEGRRAHSHVTLLNGFHETWPIQHAEDAFAFAKTGQTIVNVPDAKLVKLY
                     VDDEPLQLETADISSYERSIDFRHGELVRSFLWHTPSGKRVGITSTRLVSLVHRHLAV
                     IEYRLTLHDAAPVVVSSELLNRQDLPDDSMSVAAAGMDPRKSRAFGRRVLLPRHHSIH
                     GGQALLAYQCANSGMTMACGYEHLVQTTCEHTVEHTADPDQAKTIISLRAEAGDTVRI
                     VKFIAYHSSGSRSAGGTAGGTGNSGDSTPPSELAHRCTWTIQRAVDDGIDRIRAEQSQ
                     WLADFWDRSDIRIDGDPPAQQAMRWNLFQLAQACARSHEGGIAAKAVTGAGYEGHYFW
                     DTEIYIVPFLSYTSPEMARRLVRFRWHMLDAARQRARELNQRGALYPWRTINGEEASA
                     YYAAGTAQYHINAAVALALKRYLDASGDMGFLATEGAEILVETARMWEDLGFWATNGS
                     RQFHIHGVTGPDEYTTVVNNNLYTNVMARFNMRYASRVVESLQEWNAEAYADLARRVQ
                     LEPGEPDRWTEASEAMFLPFDETLGFHPQDDSFLQREPWDFANTPLDKYPLLLHFHPL
                     VIYRFQVLKQADVVLAMVLRSDQFTLDQKRRNFDYYDPITTGDSSLSACVQAMAASQI
                     GYEGHAVDYFRRALFVDLADTHGNTADGVHIASAGGVWGSVVFGFAGLYDSGVSLQFQ
                     PRLPREWPAMSFRLQRHGVRMHVELDNDGCTVSVLGEGAVPIETPAGVVEIDADNPIR
                     IDTTPLPV"
     gene            complement(2949..>3430)
                     /locus_tag="PLV68_10420"
     CDS             complement(2949..>3430)
                     /locus_tag="PLV68_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HRE01705.1"
                     /translation="PGSIAVLDHLDHAGLDRAGLDRPGLDYHGVATAVVSSSRNARAV
                     LDAAGIAHRFTVVVDGNVAIERGLSGKPAPDTFLDAARQLGVDPTRTAVVEDAVSGVA
                     AARAGGFALVLGVDRGANREALLSAGADMVVTDLADTLPTHHDSRNHGRNDTRWGSS"
     assembly_gap    3431..3458
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3459..3744)
                     /locus_tag="PLV68_10425"
     CDS             complement(<3459..3744)
                     /locus_tag="PLV68_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase"
                     /protein_id="HRE01706.1"
                     /translation="MLFDLDGVLTPTAAVHERAWTAMFDRFLATAAPDQPGFSAEDYR
                     RYVDGRPRFDGVQAFLVSRGLSLPEGTESDPGGLTTVGALGNLKNDLFNEA"
     assembly_gap    3816..3885
                     /estimated_length=70
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          623 a         1303 c         1480 g          657 t
ORIGIN      
        1 gcgccagaga cggcctgacc gcagcaatac cggtaccggt ggtcggtgtc gagtgcggcc
       61 agcgccgctt cgtgttcgag cgtgaactcg gtgagggcca acacgtcggc gtcggtggcc
      121 agcaccgctc cgatcgccgc gcgggcgtcg tcggtggcct ggtacagcag gttggcgtgg
      181 gcgacacgca gaccggtttc gctctcgccg gggcgagggt cgtcgtcgcc ccagcgtcgt
      241 ggccgtttgg tggtgagcgc tggtgcgacg acggctgcca gtgcgagtgc ggcacaggtg
      301 ttgacgatgg ctgtgacgcg acgacggcgc agcaccgccg ccagcgcgtt ggcggcggct
      361 gccacaccca gcatcggcgt gaacgattgc gcgatcgcga tgaggcgcca gcgcgcccgg
      421 cgcgatcgct gggagacggc gacggtgacc gcggcaacgg tgatcgcggc actcgtcaca
      481 tcgatcgggc gcaggcgcgt tggaacgacg cggagacgga gcagacccat gtcagaccgg
      541 cagcggtgtg gtgtcgatcc gtatcgggtt gtcggcatcg atctcgacga cgcccgcggg
      601 tgtctcgatg ggtaccgcgc cctcgcccag caccgacacc gtgcacccgt cgttgtcgag
      661 ctcgacatgc atccgcactc catgtcgttg caaacggaat gacatggccg gccactcgcg
      721 cggcaggcgt ggctggaact gcagcgagac gccgctgtcg tacaagccgg caaagccgaa
      781 gacgaccgag ccccacacgc cgccggccga tgcgatgtgg accccgtcgg cggtgttgcc
      841 gtgggtgtcg gcgagatcga cgaacagggc ccggcggaag tagtcgaccg cgtgcccctc
      901 gtacccgatc tggctcgcgg ccatcgcctg gacgcacgcc gacagcgacg agtcgccggt
      961 cgtgatcggg tcgtagtagt cgaagttgcg ccgcttctga tcgagcgtga actggtcgga
     1021 gcggagcacc atcgccagca cgacgtcggc ctgcttcaac acctggaacc ggtagatcac
     1081 cagcggatgg aagtgcaaca gcagcgggta cttgtcgagc ggcgtgttgg cgaagtccca
     1141 cggctcgcgc tgcaggaagc tgtcgtcttg ggggtggaag ccgagtgtct cgtcgaacgg
     1201 caggaacatc gcctcggacg cctcggtcca ccggtcgggt tcgccaggtt cgagctgcac
     1261 ccgtcgggcc aggtccgcgt aggcctcggc gttccattcc tgcaggctct cgacgacccg
     1321 cgacgcatag cgcatgttga agcgggccat cacgttggtg tacaggttgt tgttgaccac
     1381 ggtggtgtac tcgtcggggc cggtcacccc gtggatgtgg aactgccgcg acccattggt
     1441 ggcccagaac ccgaggtcct cccacatccg tgccgtctcg accaggatct cggcgccctc
     1501 cgtggcgagg aaccccatgt cgccgctggc gtcgaggtag cgcttgaggg ccagcgcgac
     1561 cgcggcattg atgtggtact gggcggtgcc ggcggcgtag tacgcggacg cctcttcgcc
     1621 gttgatcgtg cgccacggat acaacgcgcc gcgttggttc aactcgcgcg cacgttggcg
     1681 ggcagcgtcg agcatgtgcc agcggaagcg gaccagccgc cgcgccatct ccgggctggt
     1741 gtacgacagg aatgggacga tgtagatctc ggtgtcccag aagtaatggc cctcgtaacc
     1801 ggcgccggtg accgccttgg ccgcgatgcc cccttcgtgg ctgcgggcgc aggcctgggc
     1861 gagctggaat aggttccaac gcatcgcctg ttgcgcgggc gggtcaccgt cgatgcggat
     1921 gtcgctgcga tcccagaagt cggccagcca ctggctctgc tcggcgcgga tgcggtcgat
     1981 gccgtcgtcg accgctcgtt ggatggtcca ggtgcaacgg tgcgccagtt cggacggcgg
     2041 ggtggagtca ccggagttcc cggtgccgcc agcagtcccg ccagcactcc ggctgccgga
     2101 ggagtggtag gcgatgaact tgacgatccg cacggtgtcg cccgcctcgg cgcgcaggct
     2161 gatgatcgtc ttggcctggt cggggtcggc ggtgtgctcg acggtgtgct cgcaggtcgt
     2221 ctggacgagg tgttcgtacc cgcacgccat ggtcatgccg ctgttggcgc actgataggc
     2281 caggagtgcc tgcccgccgt ggatgctgtg atggcggggc agcagcacgc ggcgaccgaa
     2341 cgcccgcgac ttgcgcgggt ccatgccggc ggcggcgacg ctcatcgagt cgtcgggcag
     2401 gtcctggcgg ttgagcaact cggaggacac gaccacgggc gccgcgtcat gcagcgtcaa
     2461 ccggtactcg atcaccgcga ggtgacggtg caccaggctg accaggcggg tcgaggtgat
     2521 gccgacgcgc ttgcccgacg gcgtgtgcca caagaacgaa cgcaccaact cgccgtgacg
     2581 gaagtcgatc gagcgctcgt agctgctgat gtcggcggtc tcgagctgca acggttcgtc
     2641 gtcgacgtac agcttgacca acttggcgtc ggggacattg acgatcgtct ggccggtttt
     2701 ggcgaacgcg aacgcgtcct ccgcgtgttg gatcggccac gtctcgtgga acccgttgag
     2761 cagggtgacg tgggagtggg cgcggcggcc ttcgtccggg ttggcgcgca tgccgatgta
     2821 gccgttggcg gtggcgaaca gcgtctcggt gacgccgaga tcggcagtcg agtactcgcg
     2881 ctcgaccagg cgccatgggt cgaccgggaa gcgatgttgc agcagttcgg tcggcgtcgc
     2941 gtgggctttc atgagctgcc ccatcgcgtg tcgttccggc cgtgattgcg gctgtcgtgg
     3001 tgtgtgggca acgtgtcggc caggtcggtg acgaccatgt ccgcgccggc cgacagcagc
     3061 gcctcgcggt tggcaccgcg atcgaccccg agcaccaggg cgaagccgcc cgctctggcc
     3121 gccgcgaccc ccgagacggc atcttccacc acggcggtgc gcgtcgggtc gacgccgagt
     3181 tggcgggcgg catcgaggaa ggtgtcgggg gcgggcttgc cggacaggcc acgctcgatc
     3241 gcgacgttgc cgtcgaccac gacggtgaag cggtgggcga tgcccgccgc gtcgagcacc
     3301 gcccgcgcat tgcgtgacga ggagacgacc gcggtggcga cgccatgata atcgagaccg
     3361 ggacgatcga gcccggcacg atcgagcccg gcatgatcga gatggtcgag caccgcgatc
     3421 gagccgggat nnnnnnnnnn nnnnnnnnnn nnnnnnnnac gcctcgttga acaggtcgtt
     3481 cttgaggttg ccgagcgccc ccaccgtggt gagtcccccg gggtcggact cggtgccctc
     3541 gggcagggaa aggccgcgcg agacgagaaa ggcctgcacg ccgtcgaagc gcggccgacc
     3601 gtcgacgtat cggcggtagt cctcggcgct gaagcccggc tggtccggtg cggccgtcgc
     3661 gaggaagcga tcgaacatcg ccgtccaggc ccgttcgtgc accgctgccg tcggggtcag
     3721 cacgccgtcg aggtcgaaca gcaccgccgc gaagcgcgac cagtcgagct gcgacgtgtc
     3781 gagctgcgac gtgtcgagct gtggcgggcc gggcgnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngctgc tagcttcacg
     3901 tcccatcgag ggggagtagc cctcccagcc gctggtcgac acgctggtcc cgagggggac
     3961 ccggtcagcg ggcccgctcc ggcgggtggg cgagaccttc gaccgcatga cgtcagcgtc
     4021 gtgtcggtcg gagacgatcc gcctctgctc gacacggccc gaaccccagg gacccacaca
     4081 tgtcgatcgc agatctgctc aaagcgttca tcaccgtctt cccggccgaa ctgccggaca
     4141 agacgatgat cgcgaccatc g
//
LOCUS       DAORNF010000244         4151 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_16054_length_4151_cov_1.665039, whole genome shotgun sequence.
ACCESSION   DAORNF010000244 DAORNF010000000
VERSION     DAORNF010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4151
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16054_length_4151_cov_1.665039"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1050
                     /locus_tag="PLV68_10430"
     CDS             <1..1050
                     /locus_tag="PLV68_10430"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRE01707.1"
                     /translation="QGLLPSAQVALAYHGELIAFETFGNATDDSRYVVFSATKAFVAG
                     AMWALIGDGLIDVSRPVADYVPEFATNGKDAVTVEQVMLHTSGFPTAPLDPLRGATSA
                     GRVEAFARWRLNWQPDTTFEYHPTSAHWVLAEIIERVSGDDFRTVVRQRVTAPAGLPV
                     VLGDTGHRAETLTPVGEPATSDELMAALGVASVDVGEVTVEALVGFNRPEAQTIGVPG
                     GGGVMRAADLALYYQAVLHNPGEMWRPDVLADVTTNVRNRHPDRITGLVANRTLGLVQ
                     AGADGLSHLRGMGRTVSPAAVGHNGAGGQIAWGDPITGLSLGYCTNGLDQHMIREARR
                     TTAIASVAGVCATSD"
     gene            1465..2046
                     /locus_tag="PLV68_10435"
     CDS             1465..2046
                     /locus_tag="PLV68_10435"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HRE01708.1"
                     /translation="MLFWMFFAHRDGYPAVRATFALTIAGCITIQALVPLAPPRFLPD
                     LGFVDAGLAYNMSVYGTGGDGLSNEVSAMPSLHWAWAIIVAVVVWRFAAGRWRWLGVA
                     HLVLTAAAVTLTANHWWLDGAVSGVIAAIAYLLQRWVRDRWRALRGPATPYDDLGIAA
                     AANVPTADVPTPDVPVPDVPIPDVPIPDVPACP"
     gene            2268..2453
                     /locus_tag="PLV68_10440"
                     /pseudo
     CDS             2268..2453
                     /locus_tag="PLV68_10440"
                     /inference="COORDINATES: protein motif:HMM:NF016553.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2687..3541
                     /locus_tag="PLV68_10445"
     CDS             2687..3541
                     /locus_tag="PLV68_10445"
                     /inference="COORDINATES: protein motif:HMM:NF013252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01709.1"
                     /translation="MQVHRRGSDVRVFTRSLHEIGERVPEIVAAARSLAIDDVVLDGE
                     ALGLDADGDPLTFQDSMQASTRLRPFFFDVLHAEGRSLIDEPLSQRKQVLRSVVPGGL
                     LLPTIDVAAADGGTGDVAVAERFAADALAAGHEGVMVKSLGSAYAAGRRGAAWRKVKP
                     VRTLDLVVLAVEWGSGRRTGWLSNIHLGARGEQGSFVMVGKTFKGMTDEMLVWQTERF
                     LALEIGREGHVVHVAPETVVEIALDGVQRSTRYPGGVALRFARVKRYRPDKTAAEADT
                     IEAVQALL"
     gene            complement(3538..>4151)
                     /locus_tag="PLV68_10450"
     CDS             complement(3538..>4151)
                     /locus_tag="PLV68_10450"
                     /inference="COORDINATES: protein motif:HMM:TIGR03085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR03085 family metal-binding protein"
                     /protein_id="HRE01710.1"
                     /translation="PTLCGDWTTRDLLAHLLVRERRPDAAGGILIRALAGHTAKVQDR
                     VAARDYTASVEQFRNGPPLWTVWALPLVGDRANLAEFFVHHEDIRRAQSGWQPRPPDA
                     ERDDALWGLLKTVGRMLYRRSPVGVVLRAANRAEIVARKGEPHVTIVGEPGEIVLHAY
                     GRADDVTRIVVEGDLDRPAWRNAPRHLGAPGLMSAPHPSPGRL"
BASE COUNT          579 a         1393 c         1494 g          685 t
ORIGIN      
        1 cagggcctgt tgccgtcggc gcaggttgcg ctggcctatc acggtgagct gatcgccttc
       61 gagacgttcg gcaatgccac cgacgacagc cgctacgtgg tgttctcagc caccaaggcg
      121 ttcgtcgcag gggcgatgtg ggcactgatc ggcgacggtc tgatcgacgt gtcgcgcccg
      181 gtggccgact acgtgcccga gttcgccacc aacggcaagg atgccgtcac cgtcgagcag
      241 gtcatgctgc acacgtcggg ctttccgacg gccccgctcg accccttgcg tggtgccacc
      301 agcgccgggc gggtcgaggc cttcgcccgc tggcggctca actggcagcc cgatacgacc
      361 ttcgagtacc accccacatc ggcgcactgg gtgctggccg agatcatcga gcgggtcagc
      421 ggcgacgact tccgcactgt cgtgcgccag cgggtgaccg cccctgccgg gttgccggtc
      481 gtgctcggcg acacgggcca tcgagccgag acgctgacgc cggtcggtga gccagcgacg
      541 tccgacgagc tgatggctgc actgggggtg gcgtcggtcg acgtcggtga ggtgacggtc
      601 gaggcgttgg tcgggttcaa ccgtcccgag gcgcagacca ttggcgtgcc cggcggggga
      661 ggcgtgatgc gtgccgctga cctggcgctg tactaccagg ctgtgctcca caaccccggc
      721 gagatgtggc gccccgacgt gctggccgac gtcaccacca acgtccgcaa ccgacatccc
      781 gaccggatca ccggactggt cgccaaccgc acgctcgggc tggtgcaggc cggtgccgac
      841 ggcctgtcgc acctgcgcgg catgggccgc acggtgtcgc ccgcggcggt cgggcacaac
      901 ggagccggtg gccagatcgc gtggggcgac ccgatcaccg gcctgagcct gggctattgc
      961 accaacggcc tcgatcagca catgatccgc gaggctcgcc gcaccaccgc catcgccagc
     1021 gtggccgggg tctgcgcgac atccgactga gtcgggccac ccgaggcacg tgacctgagg
     1081 tgatcacctg atccggccga tctgagacgg acgccagggg tacagtcgcc ccgatgtggt
     1141 tgccctaccg ctacggcttc tggatcgctg ccgcatgcgc tgcgttggtc gtcaccgcgc
     1201 gggcgtcggg caatcgccat gcctgccggt cggcggtcgt gctgcaagag gtcggtctgg
     1261 tcatgctgct gtacggcatc tggcagtacg tccgccacct ggccgtcacc aagcacgtcg
     1321 gtgcggtcga caatgcccgc acgctgtggc ggatccagcg ttggatgcac ctgccgagcg
     1381 aggcggtgct gcagagctgg gtcatcgaca gcggcgcagc gatgcggttc ctgaacgcct
     1441 actacgcggt gctgcacggc ggccctgttg ttctggatgt tcttcgctca ccgcgacggc
     1501 tatcccgccg tgcgcgcgac gttcgccctg accatcgccg ggtgcatcac gatccaggcg
     1561 ctcgtaccgc tcgccccgcc gcgctttctc cccgacctcg gtttcgtcga cgccggtctg
     1621 gcgtacaaca tgtcggtcta cggcaccggg ggagatggtc tgtcgaacga ggtgtcggcg
     1681 atgccgtcgc tgcactgggc gtgggcgatc atcgtcgctg tcgtggtctg gcgctttgcc
     1741 gcggggcggt ggcggtggct gggcgtcgct catctcgtgc tgaccgcggc cgccgtcacc
     1801 ttgacagcca accactggtg gctcgacggc gctgtctccg gtgtcatcgc cgcgatcgcc
     1861 tacctgctgc aacgctgggt acgcgatcgc tggcgggcac tgcgcggccc cgccacaccg
     1921 tatgacgatc tcggcatcgc agccgccgcg aacgtgccca cggcggatgt ccccacacct
     1981 gatgtgcctg tacctgatgt gcctatacct gacgtgccta tacccgatgt accggcatgt
     2041 ccctgacgct ggccgaggtc gcggcgacgt cgcaggcggt ggcttcgacg ccgaagcgtt
     2101 ctgtcaaggt cgccgcgctg gccgaactgt tgcgccgctg caccccttcc gaggtgccga
     2161 tagtggtcgg cctgctcacc ggtcagccac gccaggggcg gctcggggtg ggatgggcac
     2221 atctccgacg cctcgccgac ggtgtgggtg actccgaggg cgcgaccatg acgatcgccg
     2281 agctcgacga catgttgacg acgctggccg cgctgcaggg acccgggtcg cagcaggccc
     2341 gggccgagtt gctcgaccgg atcgccgcta ccaccaatgc cgatgaacac gaattcttgc
     2401 gtcgcgtcat cggtggcgag ttgcgtcaag gtgccaatgc cggcgtgttg accgacgcgg
     2461 tggcgaaggc atcggggcaa gccctggccg acgtgcggcg ggcagcgatg ctgctcggcg
     2521 atgtgggcct tgccgccgaa cgggcgttgg cgggggagcg gttgaccgac atcggcctgt
     2581 cgccgctggt cggtgtgcag ccgatgctgg ccaccacggc agccacggtc gccgaagcgc
     2641 tgtcgggggt cggtccggcg agcgtcgaat ggaagctcga cggagcgtgc aggtgcaccg
     2701 gcgcggctcc gacgtgcggg tgttcacccg cagcctgcac gagatcggcg aacgggtgcc
     2761 cgagatcgtc gcagcggctc gctcgctggc gatcgacgat gtcgtgctcg acggggaagc
     2821 gctcggtctc gacgccgacg gcgacccctt gacgttccag gactcgatgc aggcctccac
     2881 gcgactgcgg ccgttcttct tcgacgtgct gcacgccgag gggcggtcgc tgatcgacga
     2941 gccgctgtcg cagcgcaagc aggtgctgcg ttcagtcgtt ccgggtgggc tcttgctgcc
     3001 gacgatcgac gtcgctgctg cggatggcgg caccggcgac gtcgccgtgg ccgaacggtt
     3061 cgccgccgat gcgctcgctg ccggtcacga gggcgtcatg gtcaagtcac tggggtcggc
     3121 ttacgccgcc gggcggcgtg gtgcggcctg gcgcaaggtc aaaccggtcc ggaccctcga
     3181 cctcgtcgtg ctcgccgtcg aatggggatc ggggcgccgc accggatggc tgagcaacat
     3241 tcacctcggt gcccgtggtg aacagggatc gttcgtgatg gtgggcaaga ccttcaaggg
     3301 gatgaccgac gagatgctgg tctggcagac cgagcgcttc ctggcgcttg agatcggccg
     3361 tgagggccat gtggtgcacg tcgcgcctga gacggtggtc gagatcgcgc tcgatggcgt
     3421 gcagcgctcg acccgctacc cagggggtgt cgcgctgcgc ttcgcccggg tcaagcgcta
     3481 tcggcccgac aagactgccg ccgaggccga cacgatcgaa gccgtgcagg ccttgctcta
     3541 gagacgtccc ggcgagggat gtggcgccga catgaggccg ggcgccccta gatgccgcgg
     3601 ggcgttgcgc caagctgggc gatcgaggtc gccttcgacg acgatgcgag tgacgtcgtc
     3661 ggcgcgaccg taggcgtgca acacgatctc gccgggttcg cccacgatcg tgacgtgcgg
     3721 ttcgcccttg cgggcgacga tctcggcccg gttggcggcg cgcagtacga cgccgacggg
     3781 ggagcgacgg tagagcatcc gtcccaccgt cttcagcagg ccccacagcg catcgtcgcg
     3841 ctcggcgtcc ggcgggcggg gctgccagcc ggactgtgcc cggcggatat cttcgtggtg
     3901 gacgaagaac tcggccaggt tggcacggtc gccgacgagg gggagcgccc acaccgtcca
     3961 cagcggtggc ccattccgga actgctcgac cgaggccgtg tagtcgcgcg ccgcgacacg
     4021 gtcctggacc ttggcggtgt gaccggccag cgcccggatc aggatgccgc cggcggcgtc
     4081 tgggcgccgc tcgcgcacca gcaggtgggc cagcagatcg cgcgtggtcc agtcgccaca
     4141 cagggtcggc t
//
LOCUS       DAORNF010000245         4148 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_16071_length_4148_cov_1.682629, whole genome shotgun sequence.
ACCESSION   DAORNF010000245 DAORNF010000000
VERSION     DAORNF010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4148
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16071_length_4148_cov_1.682629"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="PLV68_10455"
     CDS             complement(<1..402)
                     /locus_tag="PLV68_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome accessory factor PafA2 family
                     protein"
                     /protein_id="HRE01711.1"
                     /translation="MDRRIYGLENEYGVTCTLRGQRRLSPDEVARYLFRRVVSWGRSS
                     NVFLQNGARLYLDVGSHPEYATPECDSLYDLVVHDRAGERILESLVESAEQRLREEGI
                     RGTIYLFKNNTDSAGNSYGCHENYLTSRRDDL"
     gene            complement(419..1102)
                     /gene="prcA"
                     /locus_tag="PLV68_10460"
     CDS             complement(419..1102)
                     /gene="prcA"
                     /locus_tag="PLV68_10460"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit alpha"
                     /protein_id="HRE01712.1"
                     /translation="MNMPFYVAPEQVMKDRADYARKGIARGRAMVALRYVDGIALVAE
                     NPSNTLRKISEIYDRIAFAGVGKYNEFDQLRVAGVRAADLKGYQYSRDDVDARSLANS
                     YAQMLGQIFTHEMKPMEVEILVAEVALPGSDDDQLFHILYDGTVMDESRFSVLGGDAE
                     AISGRLNERFAEGLVLGDAVRAAVAALAGPDRTLTAGDLEVAMLERDVGRRCFTRLDD
                     AAVAALLGS"
     assembly_gap    1183..1246
                     /estimated_length=64
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1247..1451)
                     /locus_tag="PLV68_10465"
     CDS             complement(<1247..1451)
                     /locus_tag="PLV68_10465"
                     /inference="COORDINATES: protein motif:HMM:NF012452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit beta"
                     /protein_id="HRE01713.1"
                     /translation="MPIFAGFDLRRGTGRLWAYDATGGRYEELEQTATGSGSLHAGTA
                     IKVGYRHDMSRDEAVELACFALWQ"
     assembly_gap    1453..1589
                     /estimated_length=137
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1590..1864)
                     /locus_tag="PLV68_10470"
     CDS             complement(<1590..1864)
                     /locus_tag="PLV68_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit beta"
                     /protein_id="HRE01714.1"
                     /translation="MPIFSAGDDPGANFPELLRRVGLTHSAPTASVGADLAAPHATTC
                     VAMRYADGVVMAGDRRATAGNLISHRRMEKVVQADRHSGVAIAGTAGP"
     gene            complement(1871..2071)
                     /locus_tag="PLV68_10475"
     CDS             complement(1871..2071)
                     /locus_tag="PLV68_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like protein Pup"
                     /protein_id="HRE01715.1"
                     /translation="MAERTQKPKAAPSRTQDTDVETDAAPVAPQGERLKAELDDLLDE
                     IDEVLETNAEEFVKSYVQKGGQ"
     gene            complement(2168..>2663)
                     /locus_tag="PLV68_10480"
     CDS             complement(2168..>2663)
                     /locus_tag="PLV68_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="proteasome accessory factor PafA2 family
                     protein"
                     /protein_id="HRE01716.1"
                     /translation="GAEAGADVLARWETVLDALATDRGRVAHWVDWVAKERLITGYAE
                     RHGVAPGDARLKALDLQYHDMRPDKGLAARMQLETIADPDDVAHAMSNPPTTTRAYFR
                     GRCLRKWPDEIVAANWDSMVFDIGDDPLRRVPMEPLRGSAAHVGALLNECDTAAELLA
                     RLGS"
     assembly_gap    2664..2692
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2693..3646)
                     /locus_tag="PLV68_10485"
     CDS             complement(<2693..3646)
                     /locus_tag="PLV68_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome accessory factor PafA2 family
                     protein"
                     /protein_id="HRE01717.1"
                     /translation="MAIAKVCGIETEYGIVVRGAESNPVAASSVLINAYVSGVLGRVG
                     WDFEDEQPANDARGFSYQPVFAPEVDTQLVNAVLTNGARYYVDHAHPEISTPECVDAT
                     EVVRWDRAAEEIIRVSMQRARRLLDDGSEIVVYKNNSDGKGNSYGCHENYLMDRATPF
                     GRIVSQVTPHFVTRQVFCGAGKVGAELAHGNRDEVPFQLSQRADFFEEEVGLETTIKR
                     PIVNTRDEPHADPAKYRRLHVIVGDANMAEVATLLKVGTTAIVLAMIEDDAIGADLAL
                     ANPVAAIRQVSHDPALTRTIMLRDGRRMSALDIQWALFERAQ"
     gene            complement(3670..>4148)
                     /locus_tag="PLV68_10490"
     CDS             complement(3670..>4148)
                     /locus_tag="PLV68_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="proteasome ATPase"
                     /protein_id="HRE01718.1"
                     /translation="QQIFARYLTDEIPIAADEEIPAMIEATVAEMYRADDANRFLEVT
                     YQNGDKEVLYYKDFASGAMIENIVRRAKKLAIKRVIAGGARGVSTSDLLESIKQEYKE
                     HEDLPNTTNPDDWAKISGKKGERIVFIRTLVNEARQSSTALEGGRAIERITTGQYL"
BASE COUNT          575 a         1285 c         1341 g          717 t
ORIGIN      
        1 gaggtcgtcg cggcggctgg tgaggtagtt ctcgtggcac ccgtagctgt tgccggccga
       61 gtcggtgttg ttcttgaaca gatagatcgt gccgcggatg ccctcttcgc gcaggcgctg
      121 ctcggccgac tcgacgaggc tctccaagat ccgctccccg gcccggtcgt gcaccaccag
      181 gtcgtacagc gaatcgcatt cgggcgtggc gtattcgggg tgggagccga cgtcgaggta
      241 cagccgggca ccgttctgga ggaacacgtt gctgctgcgg ccccaactga cgacacggcg
      301 gaagagatag cgagcgacct cgtcggggct cagccgccgt tggccgcgca gggtgcaggt
      361 gaccccgtac tcgttctcga gcccgtagat cctgcggtcc atacgggcaa cctaacgcct
      421 aggaaccgag cagtgcggcg actgcggcgt cgtcgaggcg ggtgaagcag cgccgcccga
      481 cgtcgcgctc gagcatcgcc acttccaagt cgcccgcggt gagggtgcgg tcgggcccgg
      541 cgagggcagc gacggcggcc cgcactgcgt cgccaagtac gagaccttcg gcgaaccgct
      601 cgttgagccg gcccgagatc gcctcggcat cgccaccgag cacgctgaaa cgactctcgt
      661 ccatgacggt gccgtcgtag aggatgtgga acagctgatc gtcgtcgctg ccgggcaagg
      721 ccacttcggc gacgaggatc tcgacctcca tcggcttcat ctcgtgggtg aagatctgcc
      781 ccaacatctg ggcgtagctg ttggccaggc tgcgtgcgtc gacgtcgtcg cggctgtact
      841 ggtagccctt caggtcggcg gcacgcaccc cggcgacgcg caactggtcg aactcgttgt
      901 acttgccgac acccgcaaac gcgatgcggt cgtagatctc gctgatcttg cgcaacgtgt
      961 tggacgggtt ctcggccacc agcgcgatgc cgtcgacgta gcgcagcgca accatggccc
     1021 ggccgcgggc gatgcccttg cgggcatagt cggcacggtc tttcatgacc tgctcgggtg
     1081 cgacgtagaa aggcatgttc atggggtgct cctcgcggcg atcgtggcga agcgctcggc
     1141 cagctcgccg tccaccatcg gcagcaggcg gtcctgcatc tcnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncctg ccacagtgcg
     1261 aagcacgcca gttcgacggc ctcgtcgcgg ctcatgtcgt gccggtagcc gaccttgatg
     1321 gcggtgcccg catgcaggct gcccgagccg gtggcggtct gttcgagctc ttcgtaacgg
     1381 ccgccggtgg cgtcgtaggc ccacagccgg ccggtgcccc gacgcaggtc gaaaccggcg
     1441 aagatcggca cgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnng gtccggcggt gcccgcgatg gcgacaccgc
     1621 tatggcggtc ggcctggacc accttctcca tccgccgatg gctgatgagg ttgcccgccg
     1681 tcgcccgccg gtcaccggcc atcacgacgc cgtccgcgta ccgcatcgcg acgcaggtgg
     1741 tcgcgtgggg tgcggcgaga tcggcgccga cgctggccgt cggggcgctg tgggtgagcc
     1801 ccacccggcg caacagctcg gggaagttgg cccccgggtc gtcaccagcg gagaagatcg
     1861 gcatcgacat ctattggccg cccttttgta cgtagctctt cacgaattct tcggcgttgg
     1921 tctcgagcac ctcgtcgatc tcgtcgagca gatcgtcgag ttcggccttg agccgctcgc
     1981 cctgtggcgc caccggagcc gcatcggtct cgacgtcggt gtcttgcgtg cggctcggcg
     2041 ccgccttggg tttctgggtc cgttcagcca ttggaagtcc tcccccggtc ggtcggtcag
     2101 cgtggccaca ggtcgaacgt cgatcgacgg ccaactgaca gttgcaccgt atcggagcga
     2161 ccggcgatca cgaacccagc cgggccagca gttcggcggc cgtgtcgcat tcgttcaaca
     2221 gcgcgcccac gtgggcggcc gagccgcgca gcggttccat cggcacccgg cgcaaggggt
     2281 cgtcgccgat gtcgaacacc atcgagtccc agttggcagc gacgatctcg tcgggccatt
     2341 tgcgcagaca ccggccccgg aagtaggcgc gggtggtggt cggcgggttg ctcatcgcat
     2401 gggcgacgtc gtcggggtcg gcgatcgtct ccaactgcat ccgtgccgcg agacctttgt
     2461 cgggacgcat gtcgtggtac tgcaggtcga gcgccttgag ccgggcatca ccaggggcga
     2521 caccgtggcg ctcggcatag ccggtgatca gccgctcctt ggccacccag tcgacccagt
     2581 gcgcgacgcg gccgcgatcg gtggcgagcg cgtcgagcac cgtctcccat cgggcgagca
     2641 cgtcggcgcc ggcctcggcg ccgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnctgggccc
     2701 gctcgaacag cgcccattgg atgtcgagcg cggacatccg ccggccgtcg cgcagcatga
     2761 tcgtgcgggt caatgcagga tcgtgactga cctggcggat cgcggcaacg gggttggcca
     2821 acgccaggtc ggcgccgatc gcgtcgtctt cgatcatggc cagcacgatc gccgtggtgc
     2881 cgaccttcaa caaggtggcg acctcggcca tgttggcgtc gccgacgatg acgtgcagac
     2941 ggcggtactt ggcgggatcg gcgtggggct cgtcacgcgt gttgacgatt ggtcgtttga
     3001 tcgtggtctc caggccgacc tcttcttcga agaagtcggc gcgctgactg agctggaacg
     3061 gcacctcgtc gcggttgccg tgtgcaagct cggcgccgac cttgcccgcc ccacagaaca
     3121 cctgacgggt gacgaagtgc ggggtgacct ggctgacgat gcggccgaac ggggtggccc
     3181 ggtccatcag gtagttctcg tggcagccgt agctgttgcc cttgccgtcg gaattgttct
     3241 tgtagacgac gatctcgctg ccgtcgtcga gaagccgccg ggcgcgctgc atgctgactc
     3301 ggatgatctc ctcggcggca cgatcccagc gcacgacctc ggttgcgtcg acgcattcag
     3361 gtgtggagat ctcgggatgg gcgtggtcga cgtagtagcg ggcaccgttg gtgagcacgg
     3421 cgttgaccag ttgcgtgtcg acctcgggcg cgaacaccgg ctggtagctg aacccgcggg
     3481 cgtcgttggc cggctgctcg tcttcgaagt cccacccaac ccggccgagc acacctgaca
     3541 cgtaggcgtt gatcagcacg ctcgacgcgg cgacggggtt cgactccgct ccccgcacca
     3601 cgatgccgta ctcggtttcg atgccacaga ccttggcgat cgccatggac ggaccataac
     3661 ccagacgggt cagagatatt ggccggtcgt gatccgctcg atcgcccggc cgccttccag
     3721 cgcggtggag ctctgcctgg cctcgttgac cagtgtgcgg atgaagacga tccgctcccc
     3781 cttcttgccg ctgatcttgg cccagtcgtc ggggttggtg gtgttgggca ggtcttcgtg
     3841 ctctttgtac tcctgcttga tcgactcgag caggtcggag gtgctgaccc cgcgggcgcc
     3901 accggcgatg acccgcttga tcgccaactt cttggcccgc cggacgatgt tctcgatcat
     3961 cgcgcccgac gcgaagtcct tgtagtacaa cacttccttg tcaccgttct ggtaggtgac
     4021 ctccaagaag cggttggcgt cgtcggcgcg gtacatctcc gcgacggtcg cctcgatcat
     4081 cgccgggatc tcctcgtccg cggcgatcgg gatctcgtcg gtgaggtagc gggcgaagat
     4141 ctgctgtg
//
LOCUS       DAORNF010000246         4142 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_16098_length_4142_cov_1.754588, whole genome shotgun sequence.
ACCESSION   DAORNF010000246 DAORNF010000000
VERSION     DAORNF010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4142
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16098_length_4142_cov_1.754588"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="PLV68_10495"
     CDS             complement(<1..189)
                     /locus_tag="PLV68_10495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01719.1"
                     /translation="MPITDLAAPSRRASDTQSLRRGQSRWPWLAGTAAAVVVAVVVTT
                     LVVGSSPGRHVDEPPVTPE"
     assembly_gap    235..349
                     /estimated_length=115
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(365..883)
                     /locus_tag="PLV68_10500"
     CDS             complement(365..883)
                     /locus_tag="PLV68_10500"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRE01720.1"
                     /translation="MRTRESQPDRAGFEALYRHELSRMTAIGTALTGNRESGADIAHE
                     AMLRAYRDWARVSTLDQPGAWLRRVVINLATDLHRRRERERRAISRLSAERPAPAAKA
                     ADGEFWTAVRALPERQRRVVALYYIDDMAVADIARVLEIAPGTVKSALFDARRALATT
                     LGAEYARDEEVS"
     gene            complement(925..1824)
                     /locus_tag="PLV68_10505"
     CDS             complement(925..1824)
                     /locus_tag="PLV68_10505"
                     /EC_number="2.4.1.266"
                     /inference="COORDINATES: protein motif:HMM:NF010496.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl-3-phosphoglycerate synthase"
                     /protein_id="HRE01721.1"
                     /translation="MSPSTLNTVSPIRTYDASAATVADALAAKGSRTVSVCIPCKNEA
                     GTIGTLVEMLRSQLVDRHGLVDELIVLDDNSTDDTAAIANRHGARVVPIATINDRYGP
                     GTGKGNALWATVLASSGDFIVWCDGDVTTVEPSWFVGLLMPLLTTDDIALVKATYHRP
                     TEHGGGGRTTELVARPLISLFMPELSGLHQPLSGEFAGRRTAIEEIPFVQGWGVEIAM
                     LADIAERFGPASIAQVDVGDRLHRHQGLDTLGVQAAEVMATLLSRVGADKFFAREQLA
                     LLRPNGAAEPLNLDERPPIRVLR"
     gene            1863..2624
                     /locus_tag="PLV68_10510"
     CDS             1863..2624
                     /locus_tag="PLV68_10510"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HRE01722.1"
                     /translation="MELFAVLNASPDSLNTDSIVTTVDQALARAAMMLAQGADALDLG
                     GQGSTDVAEVIGWRDEWARLEPLVPALAALGVPLSVDTWRPQVARRALEAGATVMNAA
                     DGMQTDEMWQVAADFGVPIVVPFLSGPNPREMAHVTADPIEALIEFFEARLADADRFG
                     VRGNCIIDPGTGFAPSNWPWEERYLYQKIVYSNLDALRRWDLPLYIALPWKDTPQHWE
                     LMEIVLSQRPEYGRCHYPDQVRAVERRLGLDAPGV"
     gene            complement(2637..>3168)
                     /gene="folK"
                     /locus_tag="PLV68_10515"
     CDS             complement(2637..>3168)
                     /gene="folK"
                     /locus_tag="PLV68_10515"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HRE01723.1"
                     /translation="PPIAEDIQFTAVRIRRSRAELQIPPRTSHRAIVALGSNLGDRIG
                     YLKLAVAELGSSIIGQSQVFETAPVGGPDDQGAYLHMVDPFAMLRRCQRIEALALRQR
                     IVRWGPRTLDVDLLLYDDVAVQSAELTLPHPRYHERRFVLSPLSEVAPEFCPPDWDAT
                     LPPDSITALGPLDELY"
     assembly_gap    3169..3197
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3198..3502)
                     /locus_tag="PLV68_10520"
     CDS             complement(<3198..3502)
                     /locus_tag="PLV68_10520"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: protein motif:HMM:NF014233.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HRE01724.1"
                     /translation="MTNRATVPKSPSDRIEIAGLRVMTLVGVLPHEREAAQPLKFDIV
                     LEGDLRDAGSSDDLTDTAHYGLVAERAAAIARESKDELLERLVHRIAEDALTVDRGEA
                     "
     gene            complement(3584..>3763)
                     /locus_tag="PLV68_10525"
     CDS             complement(3584..>3763)
                     /locus_tag="PLV68_10525"
                     /inference="COORDINATES: protein motif:HMM:NF012500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HRE01725.1"
                     /translation="ALAGPTCDSADVLYEKSAYELPLALREGDLVDVLSAGAYTTTYS
                     SVGFNGFAPLAEYFV"
     assembly_gap    3764..3776
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3777..>4142)
                     /locus_tag="PLV68_10530"
     CDS             complement(<3777..>4142)
                     /locus_tag="PLV68_10530"
                     /inference="COORDINATES: protein motif:HMM:NF014803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01726.1"
                     /translation="ATVIRRATARGARPTFVNLGGGFPGTYREHAPGIADYGRAVNEA
                     LARWFPAQDGVRPLELMVEPGRYMVADAGVLRSEVVLVSRRSPLDSERWVYLDCGKFH
                     GLAETMDEAIRYRLRTPHDG"
BASE COUNT          604 a         1317 c         1458 g          606 t
ORIGIN      
        1 ctccggcgtg acgggcggct cgtccacgtg acgacccggc gacgaaccga ccaccagcgt
       61 ggtcaccacc acggcgacca cgaccgcggc cgcggtgccg gcgagccacg gccaccgcga
      121 ctggccgcgg cgcagtgact gcgtatccga cgcgcgtcgg ctcggcgcgg ccaagtccgt
      181 gatcggcaca tcggtgatcg aacggacggt cgccagaccg cgcccgcccg cacgnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc gtcgatcgtc
      361 gtcatcacga aacctcctcg tcacgggcgt actcggctcc cagggtggtg gccagggcgc
      421 ggcgggcatc gaacagcgcc gacttcacgg tgcccggtgc gatttccagc acccgggcga
      481 tgtcggcgac ggccatgtcg tcgatgtagt acagggccac gacgcgtcgc tggcgctcgg
      541 gcagcgctcg aacggccgtc cagaactcgc cgtcggccgc tttcgctgct ggcgccggtc
      601 gctcggcgga gagccggctg atcgcccgtc gttctcgctc gcggcggcgg tgcagatcgg
      661 tggcgaggtt gatcacgacc cgccgcagcc aggctcccgg ctggtcgagg gtgctcaccc
      721 gcgcccagtc gcgataggcg cgcagcatcg cttcgtgggc gatatcggca cccgactcgc
      781 ggttgccggt caacgcggtg ccgatcgccg tcatccggct gagctcgtga cggtagaggg
      841 cctcgaaccc cgctcgatcg ggctgcgatt cccgggtgcg catcatcacg agcatgacac
      901 gccgggatcg ccgccccggt tgggtcagcg caggacgcga atgggaggcc gctcgtcgag
      961 gttgagcggt tcggctgcgc cgttggggcg tagcagggcg agctgttcgc gcgcgaagaa
     1021 cttgtcggca ccgacgcgtg acagcagcgt ggccatcacc tcggccgctt gcacaccgag
     1081 cgtgtcgagc ccttggtggc gatgcagccg gtcgccgacg tcgacctggg cgatcgaggc
     1141 cgggccgaag cgctcggcga tgtcggccag catcgcgatc tcgacacccc agccctggac
     1201 gaacgggatc tcctcgatcg ccgtacggcg accggcgaac tcaccgctga ggggctggtg
     1261 cagcccgctc aactcgggca tgaacaggct gatcaacggc cgcgcgacca gctcggtggt
     1321 gcggccaccg ccgccgtgtt cggtgggacg gtggtatgtg gccttgacca gcgcgatgtc
     1381 gtcggtggtg agcagcggca tcagcaggcc gacgaaccac gacggctcga ccgtggtgac
     1441 gtcgccgtcg caccacacga tgaagtcgcc ggagctggcc aatacagtgg cccacagcgc
     1501 gttgcccttg cccgtgccgg ggccgtagcg atcgttgatc gtggcgatgg gaaccacccg
     1561 cgcgccgtgg cggttggcga tcgcagcggt gtcgtcggtg gagttgtcgt cgagcacgat
     1621 caattcgtcg accaggccgt ggcggtcgac gagctgtgag cgcagcatct cgaccagggt
     1681 gccgatcgtg cccgcctcgt tcttgcacgg aatgcacacg ctgaccgtgc gcgagccctt
     1741 ggccgccaag gcgtcggcga ccgtggcggc gctggcgtcg taggtgcgaa tggggctcac
     1801 cgtattgagg gtagatgggc tcatcgccaa taacctcggc gacccgaata tcctcggcct
     1861 tcgtggaact ctttgcggtg ctcaacgcct cgcccgactc gctcaacacc gactcgatcg
     1921 tgaccacggt cgaccaggct ctggccaggg cggcgatgat gctggcgcaa ggggccgacg
     1981 cgctcgacct gggcggccag ggcagcaccg atgtggccga ggtgatcggt tggcgtgacg
     2041 agtgggcgcg gctcgaaccg ctggtgccgg cgctggctgc gctgggggtg ccgctcagcg
     2101 tcgacacgtg gcgcccgcag gtggctcgcc gcgcgctcga ggcgggtgcc accgtgatga
     2161 acgccgccga cggcatgcaa accgacgaga tgtggcaggt ggcggccgac ttcggtgtac
     2221 cgatcgtggt gccgttcctg agcggcccca acccgcgtga gatggcgcat gtcacagcgg
     2281 acccgatcga ggcgctgatc gagttcttcg aggcgcggct ggccgacgcc gatcggttcg
     2341 gcgtgcgggg caactgcatc atcgacccgg gtaccggctt cgcccccagc aactggccgt
     2401 gggaagagcg atacctgtac cagaagatcg tgtacagcaa cctcgacgcg ctgcggcggt
     2461 gggatctgcc gctctacatc gcgctgccgt ggaaggacac gccccagcac tgggagctga
     2521 tggagatcgt gctgagccag cgacccgagt acgggcgctg ccactacccc gaccaggtgc
     2581 gtgccgtcga gcgacggttg ggtctcgacg ccccgggcgt ctgagatcgc cacgggtcag
     2641 tagagctcgt cgagcgggcc gagcgccgtg atgctgtcgg gcggcaacgt ggcgtcccag
     2701 tcgggcgggc agaactcggg cgccacctcg gagagcggtg agagtacgaa ccggcgctcg
     2761 tgataccgcg gatggggcag ggtgagctcg gcactttgca ccgccacgtc gtcgtacagc
     2821 agcaggtcga cgtcgagcgt gcgcggtccc cagcggacga tgcgctggcg cagggcgagc
     2881 gcctcgatcc gctggcagcg gcgcagcatg gcgaagggat cgaccatgtg gaggtacgcc
     2941 ccctggtcgt cgggcccgcc gaccggggcg gtctcgaaga cctgagactg gccgatgatg
     3001 ctggaaccca gttcggcgac ggccagtttg aggtagccga tgcgatcgcc gaggttggag
     3061 ccaagagcga cgatggcgcg gtggctggtg cggggcggga tctgcagctc ggcccggctg
     3121 cggcggatgc gcaccgcagt gaactggatg tcctccgcga tcggcgggnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnngcc tcgccccgat cgacggtgag ggcgtcttcg gcgatgcggt
     3241 ggaccagccg ttcgagcagt tcgtccttcg actcgcgagc gatcgccgca gcccgctcgg
     3301 ccaccaggcc gtagtgggcg gtgtcggtca ggtcgtcgct ggagccggcg tcgcgcagat
     3361 cgccttcgag cacgatgtcg aacttgagcg gttgcgcggc ctcgcgctcg tggggcagca
     3421 cgcccaccaa cgtcatcact cgcaggcctg cgatctcgat gcggtcggac ggtgacttgg
     3481 gcacggtggc acggttggtc atggggcgct cccttctacc ggccaagcaa tgtaccggcg
     3541 agaccggaga gagattcgtc ggcagcggcg ccgaacgaac cgctcagacg aagtactcgg
     3601 ccaacggggc gaagccgttg aagccgaccg agctataggt ggtggtgtac gcgccggccg
     3661 acaacacgtc gacgaggtcg ccttcgcgga gggcgagggg cagttcgtag gcgctcttct
     3721 cgtacagcac gtcggccgag tcgcaggtgg gtccggcgag agcnnnnnnn nnnnnngccg
     3781 tcgtggggcg tgcgcaggcg gtagcggatc gcttcgtcca tcgtctcggc caggccgtgg
     3841 aacttgccgc agtcgaggta cacccagcgc tcgctgtcga gcggcgaccg gcgcgacacc
     3901 aacaccacct cgctgcgcag cacgcccgcg tcggcgacca tgtagcgacc cggttcgacc
     3961 atcaactcca gcggtcgaac tccgtcctgc gccgggaacc accgcgccag tgcctcgttc
     4021 acggcacggc cgtagtcggc gatgccgggc gcgtgttcgc ggtacgtgcc agggaatccg
     4081 ccgcccaggt tgacgaacgt ggggcgtgcg ccgcgggcgg tcgcgcgacg gatcaccgtg
     4141 gc
//
LOCUS       DAORNF010000247         4137 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_16116_length_4137_cov_1.627389, whole genome shotgun sequence.
ACCESSION   DAORNF010000247 DAORNF010000000
VERSION     DAORNF010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4137
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16116_length_4137_cov_1.627389"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>321
                     /locus_tag="PLV68_10535"
     CDS             <1..>321
                     /locus_tag="PLV68_10535"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HRE01727.1"
                     /translation="CHLPDARIPGGADAAVAAGREAGVVQMVSVGCDRDSSLAALDAA
                     DRFGNVFATVGLHPHDATHGVASIADLFARPGVIAVGECGLDYHYDHSPRDVQRAAFA
                     EQVV"
     assembly_gap    322..376
                     /estimated_length=55
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <377..743
                     /locus_tag="PLV68_10540"
     CDS             <377..743
                     /locus_tag="PLV68_10540"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HRE01728.1"
                     /translation="ILDRESMPANTVFHCFTGGPSEAQECLARGASLSFSGIVTFKAA
                     ADVQQAAQMCPLDRMLVETDSPYLAPVPHRGRPNQPAYVAHVGTAIAGLRGEPIATIA
                     AATAANARRLFTLPLPRPT"
     gene            897..1508
                     /locus_tag="PLV68_10545"
     CDS             897..1508
                     /locus_tag="PLV68_10545"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRE01729.1"
                     /translation="MERTTDVAVIGGGAAGLSAALFVARAGLSAVVIDQERSIIKRAK
                     MHNLPGAGATEGREYLADLRGQAAAAGVSIESGKVRDVVPGDGFAVHIDGSGDADSDE
                     GSALRARYLVLASGQGTVTLPSLTLATDPPRQPFVKANVRTDRWGETSIPNVWAAGLV
                     AGWPSQVVICAGSGAAVGIEIASRDKGEFWVDHDDSSLPRDPS"
     gene            1505..>1765
                     /locus_tag="PLV68_10550"
     CDS             1505..>1765
                     /locus_tag="PLV68_10550"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRE01730.1"
                     /translation="MTVAVQATVPAVAILLPCHDEATYLPSVLDVLLAELPLHPTWRL
                     VAVNDSSTDETGAILDAAAQRAGAQMHVIHGAFGSPGRARSVA"
     assembly_gap    1766..1781
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1782..2586
                     /locus_tag="PLV68_10555"
     CDS             <1782..2586
                     /locus_tag="PLV68_10555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01731.1"
                     /translation="LGHGTHPDWLLTLDCDVEVPTGFVDGWATAIAAVDADATIGALN
                     GGEEQRHLFSGLPNAQAASSAFGRAANAAEAVVGIVNLNGVNHAVRTAGYLTAGPYVQ
                     PTVPGPGGTTNLAGSDWDLGVRIRLAGYEIGESPVTVRDRGRRLMADLFAYLSGTAYE
                     GEFARVHATGEPVDIDGALVPALADAAIDRALFHFFFKIVLADPSQLDRDLGLAPDTV
                     VAMRTWIERWPWPGFADSRNGFVYGRLPRFTAAFVADVRHQFGLDATAG"
     gene            2588..>3120
                     /locus_tag="PLV68_10560"
     CDS             2588..>3120
                     /locus_tag="PLV68_10560"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01732.1"
                     /translation="MAHTSPVTCLDHHPASGVLITGGYDGRVIATHDGHELWVATFPD
                     LVNDVRVDDRGTRVAVAVADYYAYVLDAATGWIMAALGPHGDDVNVVRWMPSTGQPST
                     GQASTDRLVCTMDHHDPTVRIWTHADGAWTCRTLAHHDSGVFGAAVSPDGSCIATAAE
                     DGTARIWDTATGTELHVL"
     assembly_gap    3121..3233
                     /estimated_length=113
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3234..>4137
                     /locus_tag="PLV68_10565"
     CDS             <3234..>4137
                     /locus_tag="PLV68_10565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01733.1"
                     /translation="DAGAAVRFVRFDPTGSHLLVGAYDATLRAYRTADWQVDREFRAP
                     FQWERAACYITTSSLDASVAVASFGAAPVFHIGGTPAGPDLPTFGINALGVREASSDG
                     GEPAVLVGRDDGRVIDTATGHTVAQHESIVNTVAISSDGRMVASGDYRGRLTVSDRMA
                     GIPLWHADVGAPINSAVWSHDGTTLYGAGYDGMIRSWTAAGELRSTHAAHHSPIKSLA
                     YTAADGGLVIAGSSDGSLSAWSGDAEAWRARGDGMVLVNSVAISAETAFVVSASRDLH
                     LRRWDVRTGELVERLPRVHDKSIKV"
BASE COUNT          573 a         1461 c         1338 g          581 t
ORIGIN      
        1 attgccacct acctgacgcg cgcatccccg gcggcgcgga cgcggccgtc gccgccgggc
       61 gcgaggcggg cgtggtgcag atggtcagcg tcggctgcga tcgcgactcg tcgctggccg
      121 cgctcgacgc ggccgatcgc ttcggcaacg tattcgccac ggtcgggttg cacccgcacg
      181 acgccacgca cggcgtcgcc tcgatcgccg acctgttcgc ccgcccgggc gtcatcgccg
      241 tcggcgagtg cggactcgac taccactacg accactcgcc ccgcgacgta cagcgagccg
      301 cgttcgcgga gcaagtggtg cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnncatc ctcgaccgcg agtcgatgcc ggccaacacc gtgttccact
      421 gcttcaccgg cggcccgtcc gaagcgcagg agtgcctggc ccggggggca tcgctgagct
      481 tcagcggcat cgtcacgttc aaggccgcgg ccgacgtgca gcaagcggca cagatgtgcc
      541 cgctcgaccg gatgctggtc gaaaccgaca gcccctatct cgcgccggtc ccccaccgcg
      601 gccgccccaa ccagcccgcc tacgtcgccc acgtcggcac cgccatcgcg ggcctgcgcg
      661 gtgaaccgat cgccaccatc gccgccgcca ccgccgccaa cgcccgtcgg ctcttcaccc
      721 tccctctacc gcgcccgacc tgatcgagcc cgaggcggca gcgagcacgg cctcgtgggc
      781 ccgccgcgtg tcgacggtgg gagggttgct gcaccgatga tgttcgctgc acgacggccg
      841 tgacggccga gaccgccccg catgcccctt caccccctca cagcttggag aatcccatgg
      901 aacgcacgac cgacgtggca gtgatcggag gaggtgcagc cgggctgtcg gcggcactgt
      961 tcgtggcccg ggcaggcttg tcggccgtcg tgatcgacca ggagcggtcg atcatcaagc
     1021 gggccaagat gcacaacctg ccgggggctg gtgcaaccga agggcgggag tacctggccg
     1081 atctgcgcgg ccaggccgcg gcggccgggg tgtcgatcga gtcgggcaag gtgcgcgacg
     1141 tggtgccggg cgacggcttc gcggtgcaca tcgacgggtc cggtgacgcc gacagcgacg
     1201 agggttccgc gctgcgggcc cgctacctgg tgctggccag tgggcagggc accgtgacgt
     1261 tgccgtcgct gacgctcgcc accgatccgc cacggcaacc gttcgtgaag gccaacgtgc
     1321 gcaccgaccg gtggggtgag acgtcgatcc ccaacgtgtg ggccgccgga ttggtggccg
     1381 gctggccatc gcaggtggtg atctgcgcgg gcagtggcgc cgcggtcggc atcgagatcg
     1441 cctcacgcga caagggtgag ttctgggtcg accacgacga ctcgtcgctg ccccgagacc
     1501 cgtcgtgacg gtcgctgtcc aggccaccgt gcccgctgtc gcgatcctgc tgccctgtca
     1561 cgacgaggcg acctatctgc cgtcggtgct cgacgtgttg ctggccgaac tgccgctcca
     1621 cccgacgtgg cgcctggtcg ccgtgaacga ctccagcacc gacgagaccg gagcgatcct
     1681 cgacgcggct gcgcagcgcg cgggcgcaca gatgcacgtc atccacggtg cgttcggctc
     1741 acccggccgg gcccgcagcg tcgcgnnnnn nnnnnnnnnn ngctcggcca cggcacacat
     1801 cctgactggc tgctgacgct cgactgcgat gtcgaagtac ccaccgggtt cgtcgacggg
     1861 tgggccacgg cgatcgcagc ggtcgatgca gatgcaacga tcggcgcgct gaacggtggt
     1921 gaagagcagc gccacctgtt ctccggtctg ccgaacgcgc aggccgcgtc gtccgcgttc
     1981 gggcgagccg ccaacgcggc cgaggccgtc gtcggcatcg tcaacctcaa cggcgtcaac
     2041 cacgcagtac gcaccgcggg gtacctcacc gcggggccct acgtgcagcc gaccgtgccc
     2101 ggccctggcg gcaccacgaa cctggccggg tccgactggg acctgggtgt gcgcatccgc
     2161 cttgccggct acgagatcgg tgaaagcccg gtcacggtgc gcgaccgggg acggcggctg
     2221 atggccgacc tgttcgccta cctcagcggc acggcgtacg aaggcgagtt cgcccgcgtc
     2281 cacgccaccg gcgagccggt cgacatcgat ggcgcgctgg tccctgcgct ggccgacgcg
     2341 gcgatcgacc gggcgctgtt ccacttcttc ttcaagatcg tgctcgccga cccgtctcag
     2401 ctcgaccgcg acctcgggct ggcaccagac acagtcgtcg cgatgagaac gtggatcgag
     2461 cgctggccgt ggcccggctt cgccgactcc cgcaacgggt tcgtctacgg ccgcctgccc
     2521 cgcttcaccg ccgcgttcgt cgccgacgtg cgccaccagt tcggcctcga cgcgacggca
     2581 ggctgacgtg gcccacacct caccggtcac ctgcctcgac caccaccccg cgagcggggt
     2641 gctgatcacc ggcggctacg acggccgcgt gatcgccacc cacgacggtc acgaattgtg
     2701 ggtggcgacg tttcccgatc tggtcaacga cgtccgcgtc gacgatcgtg gtacgcgtgt
     2761 cgctgtcgcc gtggccgact actacgccta cgttctcgac gccgccaccg gttggatcat
     2821 ggccgcgctc ggaccgcacg gcgacgacgt caacgtcgtg cgctggatgc ccagcaccgg
     2881 tcagcccagc accggtcagg ccagcaccga ccgcctggtg tgcaccatgg atcaccacga
     2941 tccgaccgtg cggatctgga cccatgctga cggcgcgtgg acgtgtcgca cgttggccca
     3001 ccacgactcg ggggtgttcg gtgccgcagt gtcgcccgac gggtcatgca tcgccacggc
     3061 cgccgaagat ggcacggcgc gcatctggga caccgccacc ggcaccgagc tgcacgtact
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnccgatgc
     3241 cggggccgcg gtgcggttcg tccgcttcga cccgaccggc agccacctgc tcgtcggcgc
     3301 ctacgacgcc acgctgcgcg cctatcgcac cgccgactgg caagtcgacc gcgagttccg
     3361 cgccccgttc caatgggagc gcgccgcctg ctacatcacc acgagttccc tcgatgccag
     3421 tgtcgcggtc gccagcttcg gtgccgcgcc ggtgttccac atcggcggca cacccgccgg
     3481 gccggatctc ccgacgttcg gcatcaacgc cctcggtgta cgcgaagcgt ccagcgatgg
     3541 cggcgaaccc gccgtactgg tcggccgcga cgacgggcga gtcatcgata cggcgacggg
     3601 gcacaccgtc gctcagcacg agtcgatcgt caacaccgtc gcgatctcgt cggacggccg
     3661 catggtggcc agtggcgact accgcggccg cctcaccgtc agcgaccgga tggcaggaat
     3721 accgttgtgg cacgccgacg tcggcgcacc gatcaactcg gcggtgtgga gccacgacgg
     3781 gacgaccctg tacggcgccg gttacgacgg gatgatccgc tcctggactg ccgccggcga
     3841 gttgcgcagc acccacgccg cgcaccactc gccgatcaag tccctcgcgt acaccgcggc
     3901 cgacggtggc ctcgtgatcg ccggctcgtc cgacgggtcg ctctcggcgt ggtcgggcga
     3961 cgccgaagcc tggcgggctc gcggcgacgg gatggtgctc gtcaactcgg tcgccatctc
     4021 cgccgagacg gcgttcgtgg tgtccgcgtc tcgcgacctg cacctacgcc gctgggacgt
     4081 gcgcaccggc gagctggtcg agcgactgcc ccgggtacac gacaagtcga tcaaggt
//
LOCUS       DAORNF010000248         4129 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_16150_length_4129_cov_1.936181, whole genome shotgun sequence.
ACCESSION   DAORNF010000248 DAORNF010000000
VERSION     DAORNF010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4129
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16150_length_4129_cov_1.936181"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(58..990)
                     /locus_tag="PLV68_10570"
     CDS             complement(58..990)
                     /locus_tag="PLV68_10570"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: protein motif:HMM:NF003915.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA];
                     GO_process: GO:0097173 - N-acetylmuramic acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HRE01734.1"
                     /translation="MFTSGAPDRTSPQPSITGANDTDPSHTVGLGTEHVDDGMDDLDL
                     RSTREVVRAMHTYDLAAVAAVGTALDTIAVVAERVAGAFVQGGRLIYVGAGTSGRLAS
                     LDASEIAPTFGTRPDQIVVIQADSEDDEAAGRTAIDRAAVGPNDVVIGIAASGRTPFV
                     VAAIEQANAAAATTVGVSCNTDAPLTAVATLAITVTTGAELVAGSTRLKAGTAQKLVL
                     NMITTAAMVTLGRTYGNRMVAMATANGKLDVRGARMVADIGQCSDERAAELLALCDGE
                     IRTAIVVHRLGLQPADARALLDRSDGSLRRALGS"
     gene            complement(1168..2358)
                     /locus_tag="PLV68_10575"
     CDS             complement(1168..2358)
                     /locus_tag="PLV68_10575"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRE01735.1"
                     /translation="MLQDRTPIVSVDDHIIEPRHVWTSRLPQRYREQGPRIVEIDGGI
                     ERWIFEGEIQATVDGATGLSAVAGTELSERMRDPGRYDNMRPGCYDPVARLADMDLDG
                     VSAQVLFPNFSRFAGMRFTTAKDKDLGLLCVRAYNDFILDEWREASPDRYIPLIILPL
                     WDVTLCVAELERCVAKGAKGLTFPDNPAMLGMPSFYTDYWNPLFAAIEAADLAICMHF
                     GSAGVAPSLAADGPAAAAAAVMGSTLFHSMSDLVFSPVLHRFSKLKILYSEGGIGWFP
                     FAAQRLDQVWEKYRFYQIVPTISADHRPSDLIRRNVFGCFIDDQLGIDLRHQIGVENI
                     TFETDYPHSDSIFPASRDALARMMADVPDDEVDKMVYGNACRLFNLDPATLVAPPTTV
                     GGRA"
     gene            2637..3260
                     /locus_tag="PLV68_10580"
     CDS             2637..3260
                     /locus_tag="PLV68_10580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01736.1"
                     /translation="MTEAGDSNTILVENDSGNVQRWFLDPSRHEIGFTIDGVEVYRAT
                     IQDEPRFDGSSLFIGTRVLRPTAGGANAAAVQAWIQAVCAALRTKAATASTPAPTAAS
                     PRPSLSAPKPAHRSSDTLRGQADKVVKAADLVTAATVLFGVIGVIGGLVLAFQTDTIE
                     TDRYSTRIERPHAITGMIIVAASIVTTAVWYTFMQCISMVGGYVREH"
     assembly_gap    3811..3918
                     /estimated_length=108
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3919..>4129
                     /locus_tag="PLV68_10585"
     CDS             <3919..>4129
                     /locus_tag="PLV68_10585"
                     /inference="COORDINATES: protein motif:HMM:NF013706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HRE01737.1"
                     /translation="LPPVPVVFTKFPSSLAGPYDDIAIVGENNDWEVELVAVVGRTAD
                     RVAEADAWDYIAGLTVGQDISDRHLQ"
BASE COUNT          695 a         1347 c         1289 g          690 t
ORIGIN      
        1 cacggacgag cagcgtgcgg atcgaggtcc cgcccgcgtc gatgccgagt gtgcgggtca
       61 cgatccgagc gcccggcgca gcgagccgtc cgatcggtcg agcagagcgc gggcgtcggc
      121 cggttgcagg ccgagccgat gcacgacgat cgcggttcgg atctcgccgt cgcacaacgc
      181 caacaactcg gccgcgcgct cgtcgctgca ctgcccgatg tcggccacca ttcgcgcgcc
      241 gcgcacgtcg agcttgccgt tggcggtcgc catcgcgacc atccggttgc cgtaggtgcg
      301 cccgagggtg accatcgccg cggtggtgat catgttgagg acgagtttct gcgccgtgcc
      361 tgccttgagc cgcgtcgacc cggcgaccag ttcggcgccg gtggtgacgg tgatcgcgag
      421 cgtggcgacg gcggtcagcg gtgcgtcggt gttgcacgac acgccgacgg tcgtcgcggc
      481 ggcagcgttg gcctgttcga tcgcggcgac gacgaacggc gttcggccgc tggcagcaat
      541 gccgatcacg acgtcgttcg gcccgactgc ggcgcggtcg atcgcggttc gcccagctgc
      601 ctcgtcgtct tccgagtcgg cctggatgac cacgatctgg tcgggtcgag taccgaaggt
      661 cggcgcgatc tccgacgcgt ccaggctcgc cagtcggccc gacgtcccgg ccccgacgta
      721 gatcagtcgc ccaccctgca cgaacgctcc ggccacccgt tcggcgacga cagcgatggt
      781 atcgagggcg gtgcccaccg cagccacagc ggccaagtca taggtgtgca tcgccctgac
      841 gacctcgcgc gtactgcgca gatcgagatc gtccatcccg tcgtcgacgt gctcggtccc
      901 cagccccacc gtgtggctcg gatcggtgtc gttcgctccg gtgatgctgg gctgggggct
      961 ggtgcggtcc ggtgcgccac tggtgaacaa gcgaataccc ccaacacgct ccagacagta
     1021 cgtggcagca gatgctatcc agcctggctc tggtgcccgt acggcagcct cgacgggtcg
     1081 ggctcgccga ggtatgtctc gaccccgagc tgatcgagac ccgcgctgcc gcgagcggtc
     1141 gcgcggcggt gccgcggcgg cgcgcctcta ggctcgccca ccgaccgtcg tcggcggcgc
     1201 gacgagcgtc gccggatcga ggttgaacag gcggcacgcg ttgccgtaga ccatcttgtc
     1261 gacctcgtcg tcaggcacgt cggccatcat ccgcgccagc gcatcgcggc tcgcggggaa
     1321 gatcgagtcc gagtgtggat agtcggtctc gaaggtgatg ttctccacgc cgatctggtg
     1381 gcggagatcg atgccgagtt ggtcgtcgat gaagcacccg aacacgtttc ggcggatcag
     1441 gtcggacggt cgatgatcgg cgctgatcgt gggcacgatc tgatagaagc gatacttctc
     1501 ccacacctgg tcgagacgct gcgccgcgaa cgggaaccag ccgatcccgc cctccgagta
     1561 gagaatcttg agcttcgaga atcgatggag gaccggcgag aacacgagat cggacatcga
     1621 atgaaacagt gtcgacccca tcaccgcggc cgcagcagcc gcgggtccgt cggcggccaa
     1681 cgacggagcc acacccgcac tgccgaagtg catgcaaatc gccaggtcgg cagcctcgat
     1741 ggcggcaaac agcgggttcc agtagtcggt gtagaacgac ggcatcccga gcatcgctgg
     1801 attgtcgggg aaggtgaggc ccttggctcc cttcgcgacg catcgctcga gctcggcgac
     1861 gcacagcgtg acgtcccaca gcggcaagat gatgagcggg atgtacctgt caggagatgc
     1921 ctcgcgccac tcgtcgagga tgaagtcgtt gtacgcccga acgcacaaca gtccgagatc
     1981 cttgtccttg gcagtcgtaa aacgcatgcc agcaaagcga gagaagttcg ggaacagcac
     2041 ctgcgccgac acaccatcca ggtccatgtc ggccaaccgg gcgaccgggt cgtagcaccc
     2101 cggacgcatg ttgtcgtagc ggccggggtc acgcatccgc tcgctgagct cagtgccagc
     2161 gacggcgctg aggcccgttg ccccgtcgac ggtggcctgg atctcccctt cgaagatcca
     2221 tcgctcgatg ccgccgtcga tctctacgat gcgcgggccc tgctctcggt accgctgcgg
     2281 aagccgggat gtccacacgt gccggggctc gatgatgtga tcgtcgactg agacgatcgg
     2341 cgtacggtct tgcagcatgt ctgcactctc ctctcgagtc ggtggcgcca gaggatgcca
     2401 ccgacaacca tacattgatt ggttttgtca ccgcaagacc cgctcgcgga acgtctgacg
     2461 ccgttcagat cccgacccgc gtggtgtcag gcagcgaacg ggccccacgg tgatggcgcg
     2521 gccagcaaca gcttcgagtg gctggcctta caggtgatac accctggccg cattgtcgtg
     2581 aagtaccttg cgaatgacgt taaaggttga agcccttgca accgactatt cgagcgatga
     2641 ctgaggcggg agacagcaac acgatcctgg tggaaaacga cagcggcaac gttcaacgat
     2701 ggttccttga cccatcacga cacgagattg ggttcacgat cgacggtgtc gaggtctatc
     2761 gagcgactat ccaggatgag ccgcgcttcg acgggtcgag cctcttcatc ggcacgcgcg
     2821 tgttgcgtcc gacggcgggt ggagccaacg ccgccgccgt ccaagcatgg attcaggcgg
     2881 tctgcgccgc cctccgtacc aaagcggcta ccgcttcgac acctgctccc accgctgcca
     2941 gccctcggcc ctcactctcg gcgccgaaac ccgctcatcg atccagcgac acgctccgcg
     3001 ggcaagccga caaggtggtc aaagccgccg acttggtcac cgctgcgacc gtgctgttcg
     3061 gtgtcatcgg tgtcatcggc ggtctcgtcc tcgcctttca aaccgacacc atcgaaaccg
     3121 accgctacag caccaggata gaacgtcccc acgccattac cgggatgatc atcgtcgctg
     3181 catcgatcgt cacaaccgcc gtctggtaca cctttatgca gtgcatcagc atggtcggcg
     3241 gatacgtccg cgagcactga ccggcgctgc gcccgcctgg agagcatcga gtcaaccatc
     3301 tcaggactgt gttggtcgcg cagccagggc caccgtgagc cacctacatt ccgcgagggc
     3361 gacctcgtcg ggctgcggct gctcggtgtc aaccccgcgg gaccacagag tgacctgcga
     3421 tatcggatcg agataaccgg catccctgct ggcgcgattg tccttccacc agctgttcag
     3481 cttgtacggc atgactccag acgtgtttgc tcgtcgatgc cggtccaggg acgcctacga
     3541 tcggcattgc ctgagaccac aagggccacc acgaccagat cagcgtgtcg ggcgtcggtc
     3601 cctgcgtgtc tgatgactca tctcgaaagg aaacccctat gcaccttgcg cactacgacg
     3661 gtcgcgctgc gctggtgttc ggcgatcgtg tcgccgacgt ggccacgatc tccaccggcc
     3721 ggttcggccc cgacccgatg gacctctacc tgcgctggga cgagttccgc gactttgccg
     3781 cgacggtcac cgctgacacc gtcgacaccg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnnnnnn nnnnnnnnct gcccccggtg ccggtcgtgt tcaccaagtt cccgtcatcg
     3961 ctggccggcc cgtacgacga catcgcgatc gtcggtgaaa acaacgactg ggaagtcgag
     4021 ctcgtcgctg tcgtcggccg caccgccgat cgtgtggccg aggccgacgc gtgggactac
     4081 atcgcggggc tgaccgtcgg ccaggacatc agcgaccgcc acctgcaac
//
LOCUS       DAORNF010000249         4129 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_16151_length_4129_cov_1.780314, whole genome shotgun sequence.
ACCESSION   DAORNF010000249 DAORNF010000000
VERSION     DAORNF010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4129
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16151_length_4129_cov_1.780314"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            69..>375
                     /locus_tag="PLV68_10590"
     CDS             69..>375
                     /locus_tag="PLV68_10590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01738.1"
                     /translation="MSAAPAGNTSRGEGGRAPRAERPARSKPRQPKADAPTQQEEPDV
                     SAEQVADEAAKFLTDLIAAFGLTGEVTSNEDGDDIEMSVTGDDLGLLVGPRGNTLLAI
                     "
     assembly_gap    376..408
                     /estimated_length=33
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<409..637)
                     /locus_tag="PLV68_10595"
     CDS             complement(<409..637)
                     /locus_tag="PLV68_10595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01739.1"
                     /translation="MLIIAFRVGDDTVDLVERVVDDLAVGRRHRLERTRLAGADDLLG
                     HLAGEATKRLAAPLAITTDVETQPRVMVTQSA"
     gene            673..1050
                     /locus_tag="PLV68_10600"
     CDS             673..1050
                     /locus_tag="PLV68_10600"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRE01740.1"
                     /translation="MTDGDLVVVLAALRERGAIGESSLPGAIAHAASFAEAVPRDATV
                     IDLGSGGGLPGLVIAVRRPDVRITLVDRRERRTDLVRLAVAQLGVQQRVEVVTADVLT
                     LAKQSRYKHAFDVVTARSFARRV"
     gene            1098..1247
                     /locus_tag="PLV68_10605"
     CDS             1098..1247
                     /locus_tag="PLV68_10605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01741.1"
                     /translation="MSNPPTQPGIDPLDRWREAGAGDLGYRPSPTPSGQITVLVNGGR
                     GERAG"
     gene            complement(1317..1727)
                     /locus_tag="PLV68_10610"
     CDS             complement(1317..1727)
                     /locus_tag="PLV68_10610"
                     /inference="COORDINATES: protein motif:HMM:NF037170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRE01742.1"
                     /translation="MERPVMRVSGPTLDAGEPLTLAAFYQRLLGWAIVRREGPRPGYP
                     ASDGWAMLASPTGDAKLEVQWEPYYRPPVWPGADGEQLMMMHLDIGVADLDHGVAWAV
                     AQGATEAAHQPQSDVRVMLDPAGHPFCLFVDDQL"
     assembly_gap    1955..2067
                     /estimated_length=113
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2448..2466
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2793..2821
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3947..>4129
                     /locus_tag="PLV68_10615"
     CDS             3947..>4129
                     /locus_tag="PLV68_10615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01743.1"
                     /translation="MEEKDAAWIDRIQTANGLDDRLDADRRKEWTTEASRPFELCDRV
                     VSQSVLIDGRALVVRKP"
BASE COUNT          604 a         1281 c         1360 g          690 t
ORIGIN      
        1 ccagcggcgc cgaacagacc ggcgcggccg agatcgacca ctcccctgtc ggtggcgaac
       61 ctgctacggt gagcgccgct ccagcgggca acacctcacg cggtgagggg ggccgcgctc
      121 cccgagccga gcgccctgcc cgttccaagc cgcgccagcc caaggctgat gccccgaccc
      181 aacaagagga gcccgacgtg agcgcagagc aggtggccga cgaggctgcg aagttcctga
      241 cagacctgat cgcggctttc ggactgaccg gcgaggtcac cagcaacgag gacggcgacg
      301 acatcgagat gtcggtcacc ggtgacgatc tcggcctgct cgtcggaccg cggggcaaca
      361 ccctgttggc gattcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngc gccgactggg
      421 tgaccatgac acgcggctgc gtctcgacat cggtggttat cgcgagcggc gccgcgaggc
      481 gcttggtcgc ttcgcccgcc aggtggccga gcaggtcatc agcaccggcg aggcgcgtgc
      541 gctcgagccg atgccgtcgg ccgaccgcaa ggtcatccac gacgcgctca acgaggtcga
      601 cggtgtcgtc acccactcgg aaggcgatga tcagcaacgc cgcgtggtga tcgctccagt
      661 cgcggactga acatgactga cggcgacctg gtcgtcgtac tcgctgcgct gcgtgagcgt
      721 ggtgccatcg gagagtcttc actccctggc gccattgctc acgcagcgtc gtttgccgag
      781 gcggttccgc gtgatgcgac ggtgatcgac ctcggttcag ggggtggact gccgggcctg
      841 gtcatcgctg ttcgtcgccc ggacgtccgg atcacgttgg tcgaccgccg cgagcggcgt
      901 accgacctgg tccggctcgc ggtggcgcag ttgggtgtgc agcaacgggt cgaggtcgtg
      961 accgccgatg tgctcaccct ggccaagcag tctcgctaca aacacgcgtt cgatgtggtg
     1021 acagcccgtt cgtttgcacg ccgggtctga cgctgcgggc cgccactccc ctgttgcgtc
     1081 ccggcggtct cctgatcgtg tcgaatccac cgacccaacc cggcatcgac cccctcgatc
     1141 gctggcgcga agcgggtgcg ggtgacctcg gctaccgccc ctcccccacc ccttccgggc
     1201 agatcaccgt cctcgtcaac ggcggccgcg gcgagcgtgc agggtgacgg ctcgcatatt
     1261 cgatacggcc cggatggtgt ctgccgagca cgggtacggc gccgggacga cgaccgtcag
     1321 agttggtcgt cgacgaacag gcagaacgga tgcccagccg gatcgagcat cacccgcacg
     1381 tcggactgcg gctggtgagc tgcttcggta gctccttgag ccacggccca cgccacgccg
     1441 tggtcgaggt cagcgacacc gatgtcgaga tgcatcatca tcagttgctc accgtcggca
     1501 cccggccaca ccggcggtcg gtagtagggc tcccactgca cttccagctt ggcgtcgccg
     1561 gttggtgacg cgagcatcgc ccacccatcc gacgccggat agccaggtcg cgggccttcc
     1621 cggcgcacga tcgcccaccc cagtagccgt tggtagaacg ccgccaacgt aagaggctcc
     1681 cccgcatcca acgtcggccc cgagacccgc atcaccggcc gctccatgcc agcgatcgta
     1741 ctgctcccac gacatggtcg atcgatcacc ctgagcggcc gggtaccgca ccacggctcc
     1801 acgagcacgg atgccaactc tggatgcagg acggtcagcc agatcagcca tcgacgtcgc
     1861 gaccaacgcg tcctgcaccg ctgcctcatc ctccgccgac cactgccgga accacttctg
     1921 atgaggatga cgcccacggg ccaccaagtc gcccnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnngtc acgatcgcat ggcgagcctg gtggttcatg
     2101 tgaaccccag gtgcccagat tcgcccatcg atcggtcacg cttccgaaaa aaccgttgca
     2161 cgaatggggt ttggccgcat gatcatccgc cgcgacgcat cgcaatccgg tcttccgggc
     2221 caacgcggac gttgagccac cgatcgttgc atgaccattc attcggagac attcctgcac
     2281 cagccgccga agagccggga gggaccggaa tccgggcgat caacggcccg gaggaaccag
     2341 acttgccgcg gtggatcgtt ggctcggcgg cgtacacgga gtcctcgggc tggccgtgtg
     2401 tgcacgtgga acgacgagag tgttcggccg cgcgcgcgac gcagtcgnnn nnnnnnnnnn
     2461 nnnnnncgtc gttgactccg tgcgtgtcgg tgcagcctgt gcccagcgcc ccacggacgt
     2521 gatcgacccc gagtgcgcgc cacgcggcga cggctgcggt cgggtccggc gaggcggtca
     2581 cgtttccgct tgtcaaaggg tcgggtgacg cgctacccgg gttggcgcgt gacaagtcgc
     2641 ggtctttctg cccgggaatt cggagtgacc gcgtattcgg tatcgagggc tccgcagctg
     2701 ctttgattcg gcgcggtcga tcggtcagct ttggcggcgt gcagggaatc tctggctggt
     2761 cgtgtgtttc acgtggaacg gccgagtggc tcnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 ntgcgcctcg ttgtagcccg tgccgagcgt tcgacgactg tggtcggctc cgacgaggcg
     2881 gtcacgttgg ctggtcacgg agtgggtcga cgcgccaccc ggcgttcgac gcgtcgcctg
     2941 tcgcggcctc tctgaccggg aggtcggaga gaccggcgtt cgtgtatttg gggctccgcg
     3001 gcagctctgt ttcgcagcgg tggatggttg agccgcggcg gcgtgcacgg aatgctcggt
     3061 ttggccgtgt gtttcatctg caacggccga gtggtcggtc gtgcgcgcca cgcggcgacc
     3121 gcggtgtcgg ctccgacggg gcggttcacg atgttgcggg tcgcggagtc ggtcgacgcg
     3181 tgaccggcgt ttggcgcgtc cctcccccgg tctctctgac ccggagtcgg agtgacctga
     3241 gtccgggtat tgaggctcca cttgtttggc tggtcgtgtg tttcacgtgg aacggcccag
     3301 tcgtccgacc atgcgcggca cgcgacgacg gcccatagtc tctccgacag cgcggtcacg
     3361 ctgtgctggt cacggagtcg gtccgcgaca cccgaccttc ggggtgatct gagttcgggc
     3421 atcgaggctc cgcggcgctc tgattcgccg cggtgaacgg ttgagccgcc gcggcgtgca
     3481 cggaatcttc gggctgaccg tgtgtttcac gtggaacggc ccggttgtac cgccgtgtcc
     3541 gctacgtacg caccgcgcgg tgcgaatgtg ctctgcggaa tgagtcccgt gatggggtgg
     3601 aggaggcggt accagtcctc gacgattcac aattcggttt ggaggagatc gcagcgcgcc
     3661 tcagcgccgc cggcgggcgt tgactgaggc cgaactcacc gcgcgtccga tgagcggacg
     3721 ccgaaccgga cctcgcgagg caacggccgc tttcggaacg gctcctttcc cgggcgggtg
     3781 ctggcgtgag tcgcgttcgg atgccctcga ttgggcggac ccgtgggaat gtggcgggat
     3841 atgtcgtgcg tccgtgagtt tcagggcggt gctgctcgaa cggcgaggct cgctgatgac
     3901 cagccaatcg tagcatgagt ggagtcaggg atggcttgcg gtgtggatgg aggagaagga
     3961 cgccgcgtgg atcgaccgca tccagaccgc gaacggcctg gatgatcgat tggatgccga
     4021 tcggcgcaag gagtggacga cagaggcatc acgtccattc gagctctgcg atcgcgtggt
     4081 gtcgcagtcg gtgctgatcg acgggcgtgc gctggtggta cgtaaaccg
//
LOCUS       DAORNF010000250         4124 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_16179_length_4124_cov_1.659867, whole genome shotgun sequence.
ACCESSION   DAORNF010000250 DAORNF010000000
VERSION     DAORNF010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4124
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16179_length_4124_cov_1.659867"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="PLV68_10620"
     CDS             complement(<1..322)
                     /locus_tag="PLV68_10620"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRE01744.1"
                     /translation="MAPYVAAKHGVVGLTKAAALDYATAGVRVNALAPGLVATGMTKG
                     WLDDPDMRDVVVAGSQLGRPAEPEEIAGMVLYLTSDLASFATGGVYVVDGGQSAVAFR
                     DAGEV"
     assembly_gap    357..386
                     /estimated_length=30
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<387..791)
                     /locus_tag="PLV68_10625"
     CDS             complement(<387..791)
                     /locus_tag="PLV68_10625"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRE01745.1"
                     /translation="MPDSLLDFTGKVVLVTGGATGIGQATSLAFARQGATVVIGDVDD
                     RATETVRLIEEQGGTALFVRTDVTVAADVERLVQTAVDKYGRVHVAFNNAGILPPTGP
                     LLDQTEADWDKTIAVDLKGVFLALKYEIAHMVA"
     assembly_gap    890..1079
                     /estimated_length=190
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1080..1397)
                     /locus_tag="PLV68_10630"
     CDS             complement(<1080..1397)
                     /locus_tag="PLV68_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HRE01746.1"
                     /translation="MSNVTVPTIPTGNYTVDSGHSRIGFVARHAMVTKVRGSFNEFEG
                     SGYFDAENPANSNLRLTIQAASIDTRNADRDGHLRSNDFFDMDSYPEITFTSTTVDRI
                     DADN"
     gene            1479..1940
                     /locus_tag="PLV68_10635"
     CDS             1479..1940
                     /locus_tag="PLV68_10635"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRE01747.1"
                     /translation="MRNVGWLSEREERAWRALQFMQMRLEGALARQLVADSGLSYPDY
                     LVLIALTDQPDGRMRLFELADVLGWEKSRASHQVGRMVGRGLVDKERCHSDRRGFYVV
                     LTASGRRTIEAAAPEHVATVRRLFVDRITAEQLDVVGDAAEAVLAALDEPI"
     gene            2102..2689
                     /gene="ygiD"
                     /locus_tag="PLV68_10640"
     CDS             2102..2689
                     /gene="ygiD"
                     /locus_tag="PLV68_10640"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: protein motif:HMM:NF007914.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HRE01748.1"
                     /translation="MTDSTTMSAAFIGHGSPMNTLDRNRYTDAWATFGTCVPTPKAVL
                     VISAHWYTNATAVTAMARPRVIHDFYGFPDELFAFDYPAPGHPEVAELVAETVKPRWV
                     GLDLDSWGLDHGTWSVLAHMFPKADVPVVQLSINATQPLDYHLDLGARLAPLRQQGVL
                     VIGSGNIVHNLRRIDWNQPSGLTGTVASTTPRPPC"
     gene            2710..2964
                     /locus_tag="PLV68_10645"
     CDS             2710..2964
                     /locus_tag="PLV68_10645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01749.1"
                     /translation="MRLTDHDAFDLAVPTPDHFIPLVYIAALAAAANDTLDVLIDGPA
                     YGSISMTSHTLGADCPPTTSDTPAAALTALAAEHPEQSNI"
     gene            complement(3160..3618)
                     /locus_tag="PLV68_10650"
     CDS             complement(3160..3618)
                     /locus_tag="PLV68_10650"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HRE01750.1"
                     /translation="MPRPSSPSTSSTPDWNSPASSERPTSSTAPKRTRSKSSGVDYAL
                     DTTGIPALIRQAVDALRQRGTAAILGASRPDAVLDIPANALMQSCKTIMGVVEGDSVP
                     DVFVPQLLDLHMQGRFPIDRLVKFYDFDQINQAAADAESGATVKPILRIA"
     gene            3606..>4124
                     /locus_tag="PLV68_10655"
     CDS             3606..>4124
                     /locus_tag="PLV68_10655"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HRE01751.1"
                     /translation="MVVALGPRSGPATVDLYPFGCALVQEIVVRALFGERLAERSREI
                     AGLFQSSQAYLESPFYRQLPHPFPVGRRAAVRSDLRDLRAIVDEQMAHLRAEPSTDAL
                     DVLDALLSDGTLSDDEIRDQVITLMGAGLDTTSASLAWILWCAVLAGPDLWHRLRAEA
                     DDVLVEGEVFDET"
BASE COUNT          603 a         1291 c         1292 g          718 t
ORIGIN      
        1 cgacctctcc cgcgtctcga aacgctacgg cgctctggcc gccgtcgacg acgtacacgc
       61 cgccggtggc gaacgatgcg agatccgagg tcaggtacag gaccatgccg gcgatctctt
      121 cgggctcggc gggccggccg agttgagagc cagcgacgac cacgtcgcgc atgtcggggt
      181 cgtcgagcca acccttggtc atgccggttg ccaccaggcc tggcgcgagc gcgttgacgc
      241 ggacgcctgc ggttgcgtag tcgagggcag cggccttggt cagaccgacg acgccgtgct
      301 tggcggcgac gtacggtgcc attccgggat cggcgatgag accggcgatc gacgctnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnntgcg accatgtggg cgatctcgta cttgagcgcg
      421 aggaacaccc ccttgaggtc gaccgcgatg gtcttgtccc agtccgcctc ggtctggtcg
      481 agcagcgggc ccgtcggcgg caggatcccg gcgttgttga atgcgacgtg cacgcggccg
      541 tacttgtcga cggcggtttg taccaggcgt tccacgtcgg cggcgacggt cacgtcggtg
      601 cggacgaaca gggcggtgcc gccttgttcc tcgatgaggc gtaccgtctc ggtggcacgg
      661 tcgtcgacgt caccgatgac gaccgtggca ccttgtcggg cgaaggcgag cgaggtcgct
      721 tggccgatcc cagtcgcacc gccggtgacg agcacgacct ttcctgtgaa gtcgagcagt
      781 gaatcgggca tgatgttgtt cctctcttgc aggtggtcgg ggtcagctgg cggcgtcggt
      841 gcggatggcc gagacctcga actcgagggt gaccttctcg gcgacgagcn nnnnnnnnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng
     1081 ttgtcggcgt cgatccggtc gaccgtggtc gaggtgaacg tgatctcggg gtagctgtcc
     1141 atgtcgaaga agtcgttcga ccggaggtga ccgtcgcggt cggcgttgcg tgtgtcgatg
     1201 ctggcggcct ggatggtcag ccgcaggttc gagttggccg ggttctcggc gtcgaagtag
     1261 ccggatccct cgaactcgtt gaacgagccc cggaccttgg tgaccatggc gtgtcgtgcc
     1321 acgaagccga tccggctgtg gccggagtcg acggtgtagt tgccggtcgg gatggtgggg
     1381 acggtgacgt tgctcatggc tgttgtgctc ctctgttgtg gtggacacat catccaacat
     1441 ggtggacgta tcatctattc ccggctacga tgatgaccgt gagaaacgtc ggctggctca
     1501 gcgagcgcga ggaacgtgca tggcgtgcgc tgcagttcat gcagatgcgc ctcgagggag
     1561 cgctcgcccg ccagctcgtc gccgactcgg gcctgtcgta tcccgactac ctcgtgctga
     1621 tcgcgctcac cgaccagccc gacggccgga tgcggctgtt cgagctcgcc gatgtcctgg
     1681 gttgggagaa gagccgagca tcccatcagg tcgggcggat ggtcggacgg gggctggtcg
     1741 acaaggagag gtgccactcg gaccgacgtg gtttctacgt cgtcctcacc gcctccggcc
     1801 gtaggacgat tgaagcggcc gcgcctgagc atgtcgcgac ggttcgccgt ctgttcgtcg
     1861 accggatcac cgccgaacag ctcgatgtgg tcggcgatgc cgccgaagcg gttctcgccg
     1921 cgctcgacga gccgatctga aacacgcagg tccctgcgag tgcgaggatg acgccgtgac
     1981 cgaccaccgt gagccgttga agatcctggc gttctcgggt gttgctacgc tccgagccgc
     2041 tcaacacccg ccttgttcgc ctggctgccc cgcagatggc catacctgat cggagggatc
     2101 gatgaccgat tccacaacaa tgtcggctgc attcatcggg cacggcagcc cgatgaacac
     2161 cctcgatcgc aaccgctaca ccgacgcttg ggccaccttt ggcacctgtg tccccacacc
     2221 caaggccgtg ctggtcatct ccgcccattg gtacacgaac gccaccgcag tgaccgccat
     2281 ggcgcgccct cgggtgatcc acgacttcta cggcttccct gacgagctgt tcgccttcga
     2341 ctatcccgca ccgggtcatc cggaagtcgc tgagctggtc gccgaaaccg tcaaaccgcg
     2401 ctgggtcggc ctcgacctcg atagctgggg actcgaccac ggcacctggt cggtgctggc
     2461 tcacatgttc ccgaaggcgg acgttccggt cgtgcaactg tcgatcaacg ccacgcagcc
     2521 gctcgactat cacctcgacc tcggcgcgcg cctcgcgccc ctgcgtcagc aaggcgtgct
     2581 ggtcatcggc agcggcaaca tcgtgcacaa cctgcgtcgc atcgactgga accagccctc
     2641 gggtctgact ggaaccgtcg cttcgacgac gccgcgaccg ccctgctgag cgagagcccg
     2701 ggtgacatct tgagactcac cgaccatgac gcgttcgacc ttgccgtgcc cacccccgat
     2761 cacttcatcc cgctggtata catcgccgct ctggctgcgg ccgccaacga cacactcgac
     2821 gtgctcatcg acggccccgc ctacggctcc atctccatga ccagccatac gctcggcgcc
     2881 gactgccctc cgacgaccag cgatacccct gccgctgcgc tcacagcact cgccgccgag
     2941 catcctgaac aaagcaacat ctgatgggtt gaagaacgtc gcccgttgca gcctcgctcg
     3001 ggcgtcgcgg cactcctcac tccgtttgcc ccactcctct cgcctcactc cgttcgccat
     3061 ctcccgatgg gcgcccaatg tgctgacccg acgcgacggc ctacgagcca gatagctgga
     3121 cgagtgtgag ctgacgctga cggggccagc gcgccgatgt caggcgatac ggaggatggg
     3181 cttgaccgtg gctcctgact cggcgtcggc agcggcctga ttgatctggt cgaagtcgta
     3241 gaacttcacg agccggtcga ttgggaaccg gccctgcatg tggaggtcga ggagctgggg
     3301 gacgaacacg tcggggacgc tgtctccctc gacgacgccc atgatcgtct tgcagctctg
     3361 catgagagcg ttggcgggga tgtcgagcac ggcgtcgggg cgtgaggcgc cgaggatcgc
     3421 ggcggtcccg cgctgtcgga gcgcgtcgac cgcttgacgg atcaacgccg gaatgcccgt
     3481 cgtgtcgagc gcgtagtcga caccggagct cttcgaccgc gtccttttgg gcgccgttga
     3541 cgatgtgggt cgctccgagc tcgcgggcga gttccaatcg ggagtcgacg acgtcgacgg
     3601 cgatgatggt cgtggcactc ggtccacgta gtgggcctgc cacggtcgat ctctacccgt
     3661 tcgggtgcgc tctggtgcag gagatcgtcg tccgtgctct cttcggcgag cggctggctg
     3721 agcggtcccg cgagatcgct gggctcttcc agagctccca ggcctacctc gagtccccct
     3781 tctaccgcca gcttccccac ccgttcccgg tcgggcgtcg ggccgcggtg cgttccgact
     3841 tgcgggacct gcgtgccatc gtcgatgagc agatggcgca cctgcgggcc gagcccagca
     3901 ccgacgcgct cgatgtcctc gacgccctgc tgagcgacgg cacactcagc gacgacgaga
     3961 tccgcgatca ggtgatcacg ctgatgggcg ccggactcga caccacatca gcatcgctgg
     4021 cctggatcct gtggtgcgcg gtgcttgccg ggcctgacct ctggcaccgc ctccgcgccg
     4081 aggctgacga cgtcctcgtc gagggcgagg tcttcgacga gaca
//
LOCUS       DAORNF010000251         4113 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_16250_length_4113_cov_1.600049, whole genome shotgun sequence.
ACCESSION   DAORNF010000251 DAORNF010000000
VERSION     DAORNF010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4113
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16250_length_4113_cov_1.600049"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1101)
                     /locus_tag="PLV68_10660"
     CDS             complement(<1..1101)
                     /locus_tag="PLV68_10660"
                     /inference="COORDINATES: protein motif:HMM:TIGR01451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01752.1"
                     /translation="MLFTVTVTDDPFVDGLFLTNQVRASEGTTNAGSQVDDSIVMIEL
                     GQPELALQKGVIATNGTGAVFAPVTVGPVAFAAPGTVGAPWAAGTITTAALTATPINS
                     NLSGIDAGDLVSFAITLENTGGADAFDVVITDTLPVGFVVPPAGAGLNLRVYDGDRNA
                     VAYADLGGGIFGTGIRLNDPSATQGGLPAGRDASGPIATGANVIVIAYDLQAADTIAP
                     NTIYTNTAQVANVAGTEGGAPHPTTDLTDTATVTSRNLVADKTIVATSEAHTSGNNVT
                     IGEIARYRLLITLPEGYASTFQLVDVLPLGLTYLADGTTNVGFVCNGGCVSSSNVAIG
                     SPTLASVTNPTVALDSSAISAGPEASGTDVTFS"
     assembly_gap    1114..1268
                     /estimated_length=155
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1269..>1513)
                     /locus_tag="PLV68_10665"
     CDS             complement(<1269..>1513)
                     /locus_tag="PLV68_10665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01753.1"
                     /translation="TPTGQREADDSGAQFELAKGVLEKTIYAINGVVCPVCTNVIIEP
                     GDTVTYRFTYTLPQTDFENLVFTDYLPLPVFAATEVAT"
     assembly_gap    1514..1676
                     /estimated_length=163
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1677..3156)
                     /locus_tag="PLV68_10670"
     CDS             complement(<1677..3156)
                     /locus_tag="PLV68_10670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01754.1"
                     /translation="MRRITRPVQLTFALRFLALVGLVAAQFAGPASPVWAVPTPSTTV
                     TAPADAFIGEPVTFTVSFQNTGGPGEVGYGPYVNVELPIAGSDGDDGLTFTSASYLGS
                     ALTPLLVVPSCTGTYTHPLTGLSTACTVGTQVVVLQLPFGSFAVGQPGADIQITAALS
                     NLADLGAPLTLRATGGFAYGSIPTGNTPIIGSTATDTVTPQLFTLTKTYIGPEDETAT
                     GPNYPRQYRIEVDIADGQTLTDLDLTDVLPNSLQYLSTDAVLAAVGSPTVTDIATPGL
                     TTPGGTLTRRLSAATGTAGANDASLLFSYYVPRLDSTSAAVLDAVSGDDRLAPDDASA
                     AASWDPIDPRDPIAPATSDVTASDHVLEEQSIAIQKGVAIANDLSPAGYSPDDVIVYT
                     LDIQISDFFAFNDVVITDTFSDGQRWDASFTPTLAVSGNGFSAPAAGFDPAQVTVDIS
                     EIAGDVDPATDGTTTVTFRLSDELVARALGDRLVGGCVPASGT"
     gene            complement(3380..>4113)
                     /locus_tag="PLV68_10675"
     CDS             complement(3380..>4113)
                     /locus_tag="PLV68_10675"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01755.1"
                     /translation="NMAGKSTYLRQVAHIVLLAQIGSFVPAASAQLGLFDRIFTRIGA
                     QDEIHAGQSTFMVEMVETANILHGATVRSLLVLDEIGRGTSTYDGLSLAWAIVEHIHN
                     HPGLKAKTLFATHYHELTDLAAVLPGVRNYNVAVTEEGERVIFLHKIIPGSADRSYGI
                     HVAQLAGLPRAVIGRQEILERLEASGGRAVRLSDDAPAQMTLFPETNPLLDELKTLDL
                     NAVPPMQALAKLFEWQARFRSQAGS"
BASE COUNT          620 a         1182 c         1364 g          629 t
ORIGIN      
        1 gctaaaggtg acgtcagtgc cgctggcctc cgggccggcg ctgatcgccg acgaatccag
       61 cgccacggtc gggttggtca ccgaggccag agtcggcgag ccgatcgcca cgttcgacga
      121 cgacacgcag ccgccgttgc agacgaagcc cacgtttgtg gtgccgtccg cgagataggt
      181 cagcccgagc ggaaggacat cgaccagctg gaaggtgctg gcgtaaccct cgggcaaggt
      241 gatgagcagg cggtaacgcg cgatctcgcc gatcgtcacg ttgttgcccg aggtgtgcgc
      301 ctcagatgtg gcgacgatcg tcttgtcggc caccagattc cggctggtga ccgtcgcggt
      361 gtcggtcagg tctgtggtcg ggtgtggcgc gccaccctcg gtgccggcga cgttggccac
      421 ctgtgcggtg ttggtgtaga tggtgttcgg cgcgatggtg tcggcggcct gcaggtcata
      481 ggcgatgacg atgacgttgg cgccggtagc gatcgggcct gaggcatcgc ggccggccgg
      541 gagcccgcct tgcgtcgcgc tcgggtcgtt gagtcggatg cccgtgccga agatcccgcc
      601 gccaagatcc gcgtaggcca cggcgttgcg atcgccgtcg tagacgcgca ggttgagccc
      661 ggcgcctgcg ggcggcacga caaagccgac cggcagtgtg tcggtgatga ccacgtcaaa
      721 ggcgtcggcg ccgcccgtgt tctccagcgt gatcgcgaag ctgaccaggt cgccggcgtc
      781 gatgcccgag aggttgctgt tgatcggcgt cgccgtcaac gccgcagtcg tgatcgtgcc
      841 ggccgcccag ggcgcgccga ccgtgccggg cgccgcgaag gccactggcc cgaccgtgac
      901 cggggcgaag accgcgccgg tgccgttggt ggcgatcacg cccttttgca gggcaagctc
      961 gggctggccg agctcgatca tgacgatgct gtcgtcgacc tggctgccgg cgttggtcgt
     1021 gccctcgctg gcgcgcacct ggttggtcag gaacaggccg tcgacgaacg ggtcgtcggt
     1081 gacggtcact gtgaagagca catcgaccac cgtnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnngt cgccacctcg gtcgcggcaa agaccggcag cggcaggtag tcggtgaaga
     1321 ccaggttctc gaagtcggtc tgcggcagcg tgtaggtgaa gcgataggta accgtgtcgc
     1381 cgggctcgat gatcacgttc gtgcacaccg ggcagacgac gccgttgatg gcgtaaatgg
     1441 tcttctcgag cacgcctttg gcgagctcga actgcgcgcc gctgtcatcg gcctcgcgct
     1501 ggccggtggg cgtnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncggt
     1681 gccggaggcc ggcacacagc cgccgaccag acggtcgccc agcgcgcgcg ccacaagttc
     1741 atccgaaagc cggaaggtga ccgtggtggt gccgtcggtg gccgggtcga cgtcgcccgc
     1801 gatctcgctg atgtcgacgg tcacctgcgc cgggtcgaac cccgcggccg gggcgctgaa
     1861 gccgttcccg ctcaccgcca gggtcggcgt gaacgacgca tcccagcgtt gaccatccga
     1921 gaacgtgtcg gtgatgacca cgtcgttgaa ggcgaagaaa tccgagatct ggatatcgag
     1981 cgtgtagacg atgacgtcat cgggcgaata gccggccggg gacaggtcgt tggcgatcgc
     2041 cacgcccttc tggatcgcga tcgactgctc ctcgaggacg tgatcgctgg ccgtgacgtc
     2101 gctggtcgct ggcgcgatcg ggtcgcgcgg gtcgatcggg tcccagctgg ccgccgcgct
     2161 ggcatcgtcc ggcgccaggc gatcgtcgcc gctgacagcg tcgagcacgg cggcagaggt
     2221 cgaatccagc cgtggcacgt aatacgaaaa tagcagcgaa gcgtcattgg cgccggccgt
     2281 tccggtggcc gcgctcaggc gtcgggtcaa cgtcccgccg ggggtcgtga gccccggcgt
     2341 ggcgatatcg gtcacggtcg gcgagccgac ggcggccagg acggcatcgg tcgacagata
     2401 ctgcaggctg ttcgggagga cgtcggtcag gtcgaggtcg gtcagcgtct ggccgtcggc
     2461 gatatcgacc tcgatccggt attggcgcgg gtaattcggg ccggtggcgg tctcgtcctc
     2521 gggcccaatg taggtcttgg tcaaggtgaa gagctgaggc gtcaccgtgt cggtcgctgt
     2581 cgagccgatg atcggcgtgt tgccggtcgg gatgctgccg taggcgaagc cgcccgtcgc
     2641 gcggagcgtg agcggcgcgc cgaggtcggc caggttcgag agcgcggccg tgatctggat
     2701 gtcggcgccc ggctggccga ccgcaaagct gccgaacggc agttgcagca cgaccacctg
     2761 tgtgccgacg gtacaggccg tggagagccc agtcaggggg tgcgtgtacg tcccggtaca
     2821 ggacggcacg acaagcagcg gcgtcagcgc tgacccaaga tagctggcgc tcgtgaacgt
     2881 cagcccgtca tcgccatcgc tgcccgcgat cgggagctcg acgttgacgt agggcccata
     2941 gcccacctcg cccggcccac ccgtgttctg gaagctcacc gtgaacgtca cgggctcgcc
     3001 gatgaacgca tcggcgggcg cggtcacggt cgtgctgggt gtgggcacgg cccagaccgg
     3061 actcgccggc ccggcaaact gggcggccac caggccaacc agggccagga agcgcagggc
     3121 gaacgtcagc tggacagggc gtgtgattcg tcgcatgaaa accccttacg gtcggctcaa
     3181 gccaaaatct aaccttggcc ggattcggat agacgtctca gattacggga agcaagagtc
     3241 atgccgccat aaggggggcg tgaaaaggta tccgcgccaa caatgcctaa cgaaaagcga
     3301 aggggcgcgg agtcagcttg agccgaccca caccccttca tggcgcgtcg ttgatgtcgc
     3361 tagatcgggc gttggcccgc tatgaaccgg cctggctcct gaaccgagcc tgccactcga
     3421 aaagcttggc aagcgcctgc atcggcggca cggcgttcag atccagggtc ttgagttcat
     3481 cgagcaaggg gttggtctcg gggaagagcg tcatctgcgc cggggcgtcg tcgctcagcc
     3541 gcaccgcgcg accgccactg gcctcgaggc gctcgaggat ctcctgccgc ccgatcacgg
     3601 cgcgcggcag cccggcgagc tgggccacgt ggatgccgta cgagcgatcg gcggagcccg
     3661 ggatgatttt gtgcaggaag atcacgcgct cgccctcttc ggtgaccgcg acgttgtaat
     3721 tgcgcacgcc cggcaaaacc gcggcgagat cggtcaactc gtgatagtgc gtggcgaaca
     3781 acgtcttcgc tttgagaccc gggtgattgt ggatgtgctc gacgatcgcc caggccagcg
     3841 agagcccgtc gtaggtgctg gtgccgcgtc cgatctcgtc gagcacgagc aggctgcgca
     3901 ccgttgcgcc atgcaggatg ttcgccgtct cgaccatctc gaccatgaac gtcgactggc
     3961 cggcgtggat ctcgtcctgg gcaccgatac gggtgaagat cctgtcgaac aggccaagtt
     4021 gggccgaggc agccggcaca aagctgccga tctgggcaag cagcacgata tgcgcgacct
     4081 gccgcaggta ggttgacttg cccgccatgt tcg
//
LOCUS       DAORNF010000252         4106 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_16282_length_4106_cov_1.701802, whole genome shotgun sequence.
ACCESSION   DAORNF010000252 DAORNF010000000
VERSION     DAORNF010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4106
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16282_length_4106_cov_1.701802"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="PLV68_10680"
     CDS             <1..243
                     /locus_tag="PLV68_10680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01756.1"
                     /translation="LQQEHWRRWFAGAVGLAIAVQAFLRFEVFEHELLAGRVGGFGQR
                     VAIMIALGLGAGVALATLVELLSSPGVAEREASADE"
     gene            236..>1010
                     /locus_tag="PLV68_10685"
     CDS             236..>1010
                     /locus_tag="PLV68_10685"
                     /inference="COORDINATES: protein motif:HMM:NF016149.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopC"
                     /protein_id="HRE01757.1"
                     /translation="MSKNDGGAWRSRAWLAVWTVVLAAVLGLGFGAKPALAHAQLLST
                     DPAAGAVLDEAPAAATMTYSEAVDIGLGSVRLFNGAGKQVDIGDAYHPEGRSSEVRVD
                     LPDLAHGSYVIDWRVVSDDGHPIQGAFTFQVGPTSDLAPGVIDDIIDSAVSNGAARAG
                     LSVARGIVIAAIAVLFGGLVAVAFGIVAPTRRLRITSWIAVAAGTVFGLVLLPLEVGY
                     AKGRGLGVITDSDAWSTALGTRIGTGWLVRALIMAVVGSA"
     assembly_gap    1011..1040
                     /estimated_length=30
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1041..1881
                     /locus_tag="PLV68_10690"
     CDS             <1041..1881
                     /locus_tag="PLV68_10690"
                     /inference="COORDINATES: protein motif:HMM:NF017262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HRE01758.1"
                     /translation="AWQMTLVGATVAVGVASAFGGHGAAGRWVPLAIAATAVHVAAMA
                     VWFGGLVALTLNWAAPDDGRSGGAAGLRRFSTMALVMVILVVVSGVVQAYRQLGSWQA
                     FTESSYGGILLWKLALVALILAVASVSRKLVAIDQGRLRRAVLVEVVIAVAIVATTSL
                     LMGSNPTVAAQSGPFSVQLVEGDYLLAVQVTPARVGTNEMHLFLSNATSSLSQPDDVT
                     VRLSDPNSDLAPIDVEIARSGAGHYTAYSAQFPYAADWQLTVTARYNQFDQITFTTTV
                     KVR"
     gene            complement(1988..2641)
                     /locus_tag="PLV68_10695"
     CDS             complement(1988..2641)
                     /locus_tag="PLV68_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HRE01759.1"
                     /translation="MPRLSEVGRDDAHPFAQRMYQFLFGDRDPVVEPGTASGTPGNWW
                     TVFALVPDAFDHTTAGFQFYRSPNRKLSPKLRELGQTRAGFTVGSQFVFSQHCKACRG
                     VGFTDEQVAALPAWSVADCWSPVERAVLAYTDCLVLQQGRVPDALFDELKRHLSDEEI
                     LELTYITATYAMHAVMTKALRLEYDDVPDRVVEIADPHGNFAGLDVMAAVDDADSTN"
     gene            complement(2768..3750)
                     /gene="glpX"
                     /locus_tag="PLV68_10700"
                     /pseudo
     CDS             complement(2768..3750)
                     /gene="glpX"
                     /locus_tag="PLV68_10700"
                     /inference="COORDINATES: protein motif:HMM:NF015290.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
     gene            3852..>4106
                     /locus_tag="PLV68_10705"
     CDS             3852..>4106
                     /locus_tag="PLV68_10705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01760.1"
                     /translation="MDGVAAERCVGARELTEPRLAPDGSRLVYAVGSALGAALMVSDL
                     DGEAPRQLSSYPQPRPGRGLGGGCWCWTPDSAAVVYCGADG"
BASE COUNT          569 a         1273 c         1514 g          720 t
ORIGIN      
        1 ctgcagcagg agcactggcg gcgatggttc gccggtgcgg tcggactggc catcgcggtg
       61 caggcgttcc tgcgcttcga agtgttcgag cacgagttgt tggcaggccg ggtcggcgga
      121 ttcggccaac gcgtggcgat catgattgcg ttagggctcg gtgccggggt cgccctggca
      181 acgttggtcg aactgttgtc gagcccgggt gttgcggaga gggaagcaag tgctgatgag
      241 taagaacgac gggggtgcat ggcggagccg agcgtggctg gcggtgtgga cggtcgtgct
      301 cgcggctgtg ttgggcctcg ggttcggtgc caaaccggcg ttggcccacg cccagttgct
      361 cagcaccgac cccgctgccg gtgcggtgct cgacgaggcc ccagccgcgg caacgatgac
      421 ctacagcgag gcggtcgaca tcgggctggg cagcgtccgg ttgttcaacg gcgcgggcaa
      481 gcaggtcgac atcggcgacg cctatcatcc cgaaggccga tcgagcgagg ttcgggtcga
      541 cctgcccgac ctcgctcacg ggtcgtacgt gatcgactgg cgggtcgtgt ccgacgacgg
      601 ccacccgatc cagggagcgt tcacgttcca ggtcggtccc acctcagacc tggcgccggg
      661 tgtgatcgac gacatcatcg actccgccgt ctccaacggc gcggcccgtg ccggcctgtc
      721 cgtggcgcgg ggcatcgtga tcgccgcgat cgcggtgttg ttcggcgggc tggtggccgt
      781 ggcgttcggc atcgtcgcgc caacccggcg gctgcggatc acctcgtgga tcgcggtcgc
      841 ggcaggcacg gtgttcgggc tggtgctgct gccgttggag gtcggctacg ccaagggccg
      901 tggtctcggt gtgatcaccg acagcgacgc gtggtcgacc gcgctcggca cgcgcatcgg
      961 caccggctgg ttggtgcgcg cgttgatcat ggccgtggtc ggctccgccc nnnnnnnnnn
     1021 nnnnnnnnnn nnnnnnnnnn ggcgtggcag atgaccctgg tcggggcgac cgtcgcggta
     1081 ggcgtcgcgt ctgccttcgg cggtcatggg gccgcggggc ggtgggtgcc gttggcgatc
     1141 gctgccacag ccgtgcatgt cgccgcgatg gcggtgtggt tcggcgggtt ggtcgcgttg
     1201 acgctcaact gggcagcccc ggatgatggg cgttcagggg gcgcggccgg gttgcgccgc
     1261 ttctcgacca tggcgctggt gatggtcatc ctggtcgtgg tcagcggggt ggtgcaggcg
     1321 taccgccaac tcggttcgtg gcaggcgttc accgagtcgt cgtacggcgg catcctgttg
     1381 tggaagttgg cgctggtggc gctgatcctg gccgtcgcgt cggtcagtcg gaagctggtc
     1441 gcgatcgacc agggccggtt gcgccgggcg gtgctggtcg aggtcgtgat cgccgttgcg
     1501 atcgtggcca ccacgtcgtt gctgatggga tcgaacccca cggtcgcggc tcagagcggt
     1561 cccttctcgg tgcagttggt ggagggcgac tatctgctcg cggtgcaggt gaccccggcg
     1621 cgggtgggta ccaacgagat gcacctgttc ctgtcgaacg cgaccagcag cctgtcgcag
     1681 cccgacgatg tgaccgtgcg cctgtccgac ccgaacagcg acctggcccc gatcgacgtc
     1741 gagatcgccc gcagcggcgc cggccactac accgcctact ccgctcagtt cccctacgcc
     1801 gccgactggc agctgacggt cacggcgaga tacaaccagt tcgaccagat caccttcacc
     1861 accaccgtga aagtgaggta gtgaacccgg agcacatcac ccagagttgg ctctgtcaat
     1921 acattctgga agcactgttg ttgtccgaca tcctggcgct cgggcggatc gcctggggcg
     1981 ggtggtgtca gttggtggag tcggcgtcgt cgaccgcggc catcacgtcg agaccggcga
     2041 agttgccgtg ggggtcggcg atctcgacca cccggtcggg tacgtcgtcg tactccaggc
     2101 gcagtgcttt ggtcatcacc gcgtgcatcg cgtacgtggc ggtgatgtag gtgagctcga
     2161 ggatctcctc gtcgctcaga tgacgcttca gctcgtcgaa cagggcgtcg ggcacgcggc
     2221 cctgttgaag caccaggcag tcggtgtagg cgagcaccgc ccgctcgacc gggctccagc
     2281 agtcggcgac tgaccaggca ggtagtgcag cgacttgctc gtcggtgaag ccgacgccgc
     2341 ggcacgcctt gcagtgttgg ctgaacacga actggctgcc gaccgtgaac ccggcgcggg
     2401 tctggccgag ctcgcgcagc ttcggcgaca gcttgcggtt ggggctgcgg tagaactgga
     2461 agccggcggt ggtgtggtcg aacgcgtcgg gaaccagcgc gaacaccgtc caccagttac
     2521 cgggtgtgcc cgatgccgtg cccggttcca ccaccgggtc gcggtcgccg aacaggaact
     2581 ggtacatccg ttgcgcgaac gggtgggcgt cgtcgcggcc gacctcagag agacgaggca
     2641 cgtcacacct ccaggtgctc gagcgtcgtc gcggcgacgt tgctggcggc gtaggcactg
     2701 cgatgtaagg acatggcccg cagcgtagaa cgacccgccc ccaccaaacg ggtcgtggac
     2761 gcggcggtca gtcgtagtcg acggaggcgt agttgcggag tttgtcgatg cggtgaatgg
     2821 cttcgacggt gcgcacggtg ccggactgcg agcgggtgac caacgaatgg gtgtgggcgc
     2881 cgaggctgtc gtagcgcacg ccgcgcagca gggcgccgtt ggtgatgccc gtggcggcga
     2941 agaagcagtt gtcgccttgc accaggtcgc tggtggtcaa cacctggtcg aggtcgtaac
     3001 ccgcggcgat cgcggcggcg cgctcgtcgt cgttgcgggg atagaggcgg ccttggatct
     3061 cgccgccgat gctcttcagc gcggctgcgg ccacgactcc ttcgggggtg ccgccgatgc
     3121 cgatcagcac gtcgaccccg gcgtcgggcc agcaggtggc gatcgcgccg aagatgtcgc
     3181 cgtcgctgat caggcggatg cgcgcacccg tggcgcggac ctcttcgatc aaccccgcgt
     3241 ggcggggacg atcgaggatc atcacggtca ggtcgcgtac agcggcgtgc ttggccttgg
     3301 ccacccagcg caggttctgg gtcggcgtcg cggtgacgtc gatcgcgcca gcagcctcag
     3361 gaccgacggc gatcttttcc atgtacacgc acggcccagg gttgaacatg gtgccccgct
     3421 cgcgatgacc gccaacgcgt tgtccttgcc ctgcgcggtc agcgtggtgc cgtcgatcgg
     3481 gtcgacggca atgtcggtca gcgggggagt gccgtcaccg atccgctcac cgttgaacag
     3541 catcggtgct tcgtcctttt cgccttcacc gatgacgacg acgccgtcca tctgcaccga
     3601 ggcgaggacg gtgcgcatcg cctcgaccgc ggcaccgtcg gcaccgttct tgtcgccccg
     3661 cccgacccag cgcgatgcgg cgagagcggc agactcggta acgcggacca gttccaacgc
     3721 gaggttgcga tcaggcttct gtgcggacat gaccccaccc tatgccgtgc ccctccgccc
     3781 ataccaaggc ccccagtccc atgttggcaa tgggcatcgt cccggcgtag gcaacgcgta
     3841 gggtgctggg cgtggatggg gtggcggcgg agcggtgtgt gggggcgcgg gaactgaccg
     3901 agccgcggtt ggcgccggac gggagtcggc tggtgtacgc cgtcggctct gcgttgggtg
     3961 cggcgttgat ggtgtcggac ctcgacggcg aggcaccacg gcagttgtcg agctatccgc
     4021 aaccgcgacc cgggcgcggg ctgggcggtg ggtgctggtg ctggacgccc gactctgcgg
     4081 cggtggtcta ctgcggtgcc gacggc
//
LOCUS       DAORNF010000253         4098 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_16325_length_4098_cov_1.489488, whole genome shotgun sequence.
ACCESSION   DAORNF010000253 DAORNF010000000
VERSION     DAORNF010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4098
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16325_length_4098_cov_1.489488"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="PLV68_10710"
     CDS             complement(<1..432)
                     /locus_tag="PLV68_10710"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01761.1"
                     /translation="MTLDKLFSSFPEYSITGLVLGSIYALVALGYTLVYGVLRLINFA
                     NSEVFMLAAFGSYLAMDILNIPLRMPDGSDPNKTGMALFVTLLVCMVLSLIIGAAVAV
                     LVERVAYRPLRRRDAPRLVFLISAIGASFAIAEYVGEWGFPS"
     gene            complement(520..>1753)
                     /locus_tag="PLV68_10715"
     CDS             complement(520..>1753)
                     /locus_tag="PLV68_10715"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HRE01762.1"
                     /translation="PSISRGRTNTMHRTTTGRLGAAALGLSLVAVAACGSDKKAADTT
                     TTATTAPQSSSSAAPGGDGVTCEAGLKLAFLGALSGDNGALGTNMVNGMSIAIDDFNA
                     ANPNCQITLDRSYDSQGDPAQATPLADTIVNDAKIIGLVGPGFSGETRATMPTFEEFG
                     LPMISPGATNATLSQEGWKMFHRVLANDDKQAPGVAALIKDTIKATKVGVIDDATDYG
                     KGLAESVKTNLGSLLVESATINPDSADYSAAVTAMKNAGVDAVFYSGYYAEAAKFVSQ
                     MRDAGVTATFVSGDGSLDVGFIDNAGAASEGAYLTATGAPPDVNPEFSAKFNAKFGTD
                     PTLYSPEAYDAASVFLAGIAAGNVTRDALATFIDGYDAQGITKNLKFDATGEPEGSAV
                     FYTIVEGGKLVSKGLIGA"
     assembly_gap    1754..1763
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1857..>2601)
                     /locus_tag="PLV68_10720"
     CDS             complement(1857..>2601)
                     /locus_tag="PLV68_10720"
                     /inference="COORDINATES: protein motif:HMM:NF014665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HRE01763.1"
                     /translation="AIEQINRLFTGVVSIAVIAAVLGAFRRTPRRRDLTRLSIVIALG
                     VPMQGVVGAIVVWTDLNPFANQQHFLLSMVLVALATVLVWRAGEAEAADGSVTRRPRV
                     ADDVRRHVWVITVATAVALLSGTFVTGAGPHAGDEDAKRFDIAITTIARIHALAVLIT
                     IASAAALLWRLRNRRQDRETLDASVTTWMVLAVAQAGLGYTQYFTDVPAVLVGIHVAL
                     ATGLWMATVWLQLTTTTADSSVAAARADT"
     assembly_gap    2602..2627
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2895..>3203
                     /locus_tag="PLV68_10725"
     CDS             2895..>3203
                     /locus_tag="PLV68_10725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01764.1"
                     /translation="MLTSFDDYPIHQACVPVAHTATADINHYDRYFFNGYTKGGDVYF
                     AVAMGLYPNRHVHDAAFSIVRGDQQISVFGSGRAPRDRRDANRVGPITVDVVEPMAVI
                     T"
     assembly_gap    3204..3324
                     /estimated_length=121
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3325..>3591
                     /locus_tag="PLV68_10730"
     CDS             <3325..>3591
                     /locus_tag="PLV68_10730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01765.1"
                     /translation="TRLTQFGRWAGWIELDGERIELDEVIGSRDRSWGIRPVGEPAPS
                     GAPVALPQFFWLWAPVCFDGFSTHFDVNEYGDGRRWHEVGARVAD"
     assembly_gap    3592..3703
                     /estimated_length=112
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3704..3998
                     /locus_tag="PLV68_10735"
     CDS             <3704..3998
                     /locus_tag="PLV68_10735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01766.1"
                     /translation="TVRLQPTYNFQMSGLGYGHPRWTHGAWLGEAVVGGERITLPITD
                     PLTRESVHVQSLCQATCTAPDGTVSTGTGILEQLCIGPHPSGLTGILDPATPQ"
BASE COUNT          657 a         1262 c         1294 g          616 t
ORIGIN      
        1 cgaggggaag ccccactcac cgacatactc ggcgatcgcg aagctggccc cgatggccga
       61 gatcaagaac accaagcgtg gggcgtcgcg ccgacggagc gggcgatacg cgacccgttc
      121 gaccagcacc gcgaccgcgg cgccgatgat cagcgacagc accatgcaga cgagcagggt
      181 gacgaacagc gccatacccg tcttgttggg gtcgctgccg tcgggcatcc gcaacgggat
      241 gttgaggatg tccatcgcca gatacgaccc gaacgccgcc agcatgaaca cttccgagtt
      301 ggcgaagttg atcaatcgca acacgccgta gaccagcgtg taaccgaggg cgacgagggc
      361 atagatactg cccaacacca acccggtgat gctgtactcc ggaaacgaag agaagagttt
      421 gtcgagggtc acaggttctc ccgcatgacg gactttgtga acagcaaagg gtcggagccg
      481 acatggctcc gacccttcgc atcatgtgtg tgctcggact caggccccga tgaggccctt
      541 ggacacgagc ttgcccccct cgacgatggt gtagaacacc gccgaacctt cgggctcgcc
      601 ggtcgcgtcg aacttgaggt tcttcgtgat gccttgggcg tcgtagccgt cgatgaacgt
      661 cgccagcgca tcgcgggtga cgttgccggc ggcgatcccg gcgaggaaca cgctggccgc
      721 gtcgtaggcc tcgggcgagt acagcgtcgg gtcggtgccg aacttggcgt tgaacttcgc
      781 cgagaactcg gggttgacgt ccggcggggc gcccgtcgcg gtcaggtacg caccctcgga
      841 ggcggcaccg gcgttgtcga tgaagcccac gtcgagcgag ccgtcgcccg acacgaacgt
      901 ggcggtgacg ccggcgtcgc gcatctggct gacgaacttg gcggcctcgg cgtagtagcc
      961 cgagtagaac accgcgtcga cgccggcgtt cttcatcgcc gtgaccgcag ccgagtagtc
     1021 ggccgagtca gggttgatgg tggccgactc gaccagcagc gagccgaggt tggtcttgac
     1081 cgactcggcc aagcccttgc cgtagtcggt ggcatcgtcg atgacgccga ccttggtggc
     1141 cttgatggtg tccttgatga gggcagccac gccaggtgcc tgcttgtcgt cgttggccag
     1201 gacgcggtgg aacatcttcc acccttcctg cgacaacgtg gcgttggtgg cccctgggct
     1261 gatcatgggc agaccgaact cttcgaacgt cggcatcgtg gcgcgggtct cacccgagaa
     1321 cccgggcccg accaagccga tgatcttggc atcgttcacg atggtgtcgg ccagcggcgt
     1381 cgcctgagcc gggtcgccct ggctgtcgta cgaacggtcg agggtgatct ggcagttcgg
     1441 gttggcggca ttgaagtcgt cgatggcgat cgacatgccg ttgaccatgt tggtgcccag
     1501 ggcaccgttg tcaccagaga gcgctcccag gaaggccagc ttcaggcccg cctcgcaggt
     1561 gacgccgtcg ccgcccggag cggcactgga cgagctctgc ggcgcggtgg tcgcggtggt
     1621 ggtggtgtcg gcggccttct tgtcgctacc gcatgcggcg acggcgacaa gcgagagccc
     1681 aagcgcggct gctccgaggc ggcctgtcgt tgttctgtgc attgtgttgg ttctccccct
     1741 ggagatgctc ggannnnnnn nnnggcatga gtgccgtccc gatctgttga aggccaacac
     1801 gttagcaatc gcgatcgatg atgggagtga tcggggggat tggttgttcg tgacgatcat
     1861 gtgtcggcgc gggcggccgc aacactggaa tctgcagtcg tggtcgtgag ctgcagccac
     1921 accgtggcca tccacagccc ggtcgcgagc gcgacgtgga tgccgaccag caccgccggc
     1981 acgtcggtga agtactgggt gtaaccgagg ccagcctggg cgacggcgag caccatccag
     2041 gtcgtgaccg acgcatcgag cgtctcgcgg tcctggcgac ggttgcgcaa ccgccacagc
     2101 aacgccgccg ccgacgcgat cgtgatcagc accgcgaggg catggatgcg ggcgatcgtg
     2161 gtgatcgcga tgtcgaaccg cttcgcgtcc tcgtcgccgg cgtggggacc ggcgccggtg
     2221 acgaaggtgc ccgacaacag cgcgaccgcg gtcgcgaccg tgatcaccca gacgtgacgg
     2281 cgcacgtcgt cggcgaccct cggcctgcgc gtcaccgagc cgtcggctgc ttcagcctcg
     2341 ccggcccgcc acaccagcac ggtcgccaac gcgaccagca ccatcgacaa caggaagtgc
     2401 tgctggttgg cgaaggggtt gaggtcggtc cagaccacga tcgctcccac caccccctgc
     2461 atcggcacac cgagcgcgat cacgatcgag agccgggtga gatcgcgcct ccggggcgtg
     2521 cgccggaagg caccaaggac tgcggcgatc accgcgatgc tgaccacacc ggtgaacagc
     2581 cggttgatct gttcgatcgc gnnnnnnnnn nnnnnnnnnn nnnnnnngtg ggtcgtcgac
     2641 acgtcgatga accgttcgct gttgcagttc ggccagtcgg agcaccccag gccggaaccg
     2701 ctgaggcgca ccgacgcacc cgtgaccacg atgaggcaca ggcacaccag tgagaccacg
     2761 gtgatggtgc gatatcggcg cggcgagata cggccagaca tgacggccag ggtaggtggc
     2821 cgggccgagc ggctcccaac gcgcagatcg gctccgaccc ggggcggtga cgacggtcac
     2881 cctacgcttg cggtgtgctc accagcttcg acgattaccc gatccatcag gcctgcgtgc
     2941 cggtggctca tacggccacg gccgacatca accactacga ccgctacttc ttcaacggtt
     3001 acaccaaggg cggcgatgtg tacttcgcgg tggcgatggg gctgtacccg aatcggcacg
     3061 tccacgacgc ggcattcagc atcgtgcgcg gcgatcaaca gatcagcgtg ttcgggtcgg
     3121 gccgggcacc gcgcgatcgc cgtgacgcca accgggtcgg accgatcacc gtcgacgtgg
     3181 tcgagccgat ggcggtgatc accnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnacccgc ctcacccagt tcggacgctg ggcagggtgg
     3361 atcgaactcg acggtgaacg gatcgagctc gacgaggtga tcggttcgcg cgaccggtcc
     3421 tggggcatcc gcccggtcgg cgaaccggcg ccgagcggcg ccccggtcgc gctcccccag
     3481 ttcttctggc tgtgggcacc ggtgtgcttc gacgggttca gcacgcactt cgacgtcaac
     3541 gaatacggcg acggtcgccg ctggcacgag gtcggcgcgc gggttgccga tnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncacggtg cggctgcaac
     3721 cgacgtacaa cttccagatg agcgggttgg gctacggcca cccgcgctgg acccacggcg
     3781 cctggctcgg agaagccgtg gtcggcggcg aacgcatcac gctgcccatc accgacccgc
     3841 tcacccgcga gtcggtccac gtccagtccc tgtgccaggc cacctgcacc gcccccgacg
     3901 gcacggtgtc caccggcacc ggcatcctcg agcagctctg catcgggccc cacccctccg
     3961 gcctcaccgg cattctcgac ccggccaccc cccagtagcc cgagcaagtg cgttcgatcc
     4021 acccctgata gcagggccaa cccaacacag ttgtggcgag cgacggcaag tgtgttcact
     4081 tcaccccctg tggcgggg
//
LOCUS       DAORNF010000254         4091 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_16362_length_4091_cov_1.381070, whole genome shotgun sequence.
ACCESSION   DAORNF010000254 DAORNF010000000
VERSION     DAORNF010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4091
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16362_length_4091_cov_1.381070"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="PLV68_10740"
     CDS             complement(<1..192)
                     /locus_tag="PLV68_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase"
                     /protein_id="HRE01767.1"
                     /translation="MPQIPQGTRGAVITGWGSALPPKVLTNHDLEQMFDTSDEWIVER
                     TGIRQRHVGGSTVGLSIESG"
     gene            complement(194..>744)
                     /locus_tag="PLV68_10745"
     CDS             complement(194..>744)
                     /locus_tag="PLV68_10745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01768.1"
                     /translation="TSAQPRDTEVPVVSNVDAAPHAAGTEWASLLSAQLSSPVRWKQC
                     LQSLAGLGVSSFAELGPGGVLTGMAKRTVDSARTISVSTPEELDKLLEWINASAPRAA
                     AQLEGEHLFASERLVVSPAAGVFTPVEGITDGSTIQVGTVLGNVGDVEVRSAFAGVLQ
                     SYIAVQGERITLRQPIAWLRTV"
     assembly_gap    745..826
                     /estimated_length=82
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<827..>1116)
                     /locus_tag="PLV68_10750"
     CDS             complement(<827..>1116)
                     /locus_tag="PLV68_10750"
                     /inference="COORDINATES: protein motif:HMM:NF012901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HRE01769.1"
                     /translation="GHSLGEYTALTAGGALSFDDGVRLVCERADAMHEAGLASPGTMA
                     AVLGLDDDQVEVACRLADSEVWVANFNAPGQVVIAGSPAGVAAAGNEAKGLG"
     assembly_gap    1117..1233
                     /estimated_length=117
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1522..1604
                     /locus_tag="PLV68_10755"
     tRNA            1522..1604
                     /locus_tag="PLV68_10755"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1555..1557,aa:Leu,seq:caa)
     gene            complement(1640..>1973)
                     /locus_tag="PLV68_10760"
     CDS             complement(1640..>1973)
                     /locus_tag="PLV68_10760"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HRE01770.1"
                     /translation="DGNVVARDRLATAIATVQPDLVLPRPGDVGHDADADADTDGDGD
                     GDGDALIGPAIAAAHAALLASPAAVRLVSMDDMTASTERPNHPGTLNATNWRRRLSVS
                     VDDILAPR"
     assembly_gap    1974..2127
                     /estimated_length=154
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2128..3446)
                     /locus_tag="PLV68_10765"
     CDS             complement(<2128..3446)
                     /locus_tag="PLV68_10765"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HRE01771.1"
                     /translation="MNCDAWGISDGYFDIDGTWHPTSDETRARLRAAIGGARPGPPLW
                     FVVQGTAGSLWNECRIVTEDGLDLGIVAALPTDLPLGYHRLIPVDGGPETTLIVHPGR
                     CPDIPRAWGAATQLASLWSERSWGIGDLGDLRTLCASLHQAGGRTLLISPLHQPAPSL
                     PQEPSPYYPSSRRHLSPLAIAIEGDPASELWCRPDELIDRDAAWTAKRAALEALFEIE
                     IGSGAARADFDVAMAIMSGDLVPAWNALCDRYGPDWSSWPDGLDRPDPQALTAAFASA
                     DLTRAASFHAWCQGLAHHQLAEAAATGVAIIADLAVGFSPLGADAWQFQDLLALDMCL
                     GAPPDSFTPAGQTWGIPPFVPWRLREAGYRPFIDTIRACLQGVQALRIDHVIGLFRQF
                     WIPVDATPADGAYVQFPADELLAIVCLEATRAGAFVIGEDLGTVEPDA"
     gene            3528..3821
                     /locus_tag="PLV68_10770"
     CDS             3528..3821
                     /locus_tag="PLV68_10770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01772.1"
                     /translation="MSLSSRLVERKLRQSASRLRALRDELRVIDEQLPSLQADADDGD
                     MRALFDETGGSNAEARRARGSAEAMVRQRARVVESIATWERRQDELLDRLARR"
     gene            3873..>4091
                     /locus_tag="PLV68_10775"
     CDS             3873..>4091
                     /locus_tag="PLV68_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRE01773.1"
                     /translation="MTIRVVIAEDEAIIRLDLKETLEEEGYDVVGETGRGDKAVELVR
                     ELRPDLAILDIKMPGMDGLEAAAIINAER"
BASE COUNT          571 a         1234 c         1364 g          569 t
ORIGIN      
        1 gcccgattcg atcgacaggc cgaccgtgct gccaccgacg tggcgctgac ggatgccggt
       61 gcgctcgacg atccactcgt cgctggtgtc gaacatctgc tcgaggtcgt ggttggtgag
      121 caccttgggt ggtagcgccg aaccccaacc ggtgatcacc gcaccgcgtg tgccttgtgg
      181 aatctggggc atatcacacc gtcctgagcc atgcgatcgg ttgccgcaag gtgatccgtt
      241 caccctggac ggcaatgtaa ctctgcaaca ccccggcaaa cgcagaccgg acctcgacgt
      301 caccgacgtt gccgagcacg gtgccgacct ggatggtgga gccgtcggtg atcccctcga
      361 ccggcgtgaa cactcccgcc gcggggctga cgaccagccg ttcggacgcg aacaggtgct
      421 caccttcgag ctgcgctgcg gcccgcggcg cgctggcatt gatccattcg agcagcttgt
      481 cgagctcttc gggggtggag accgagatcg tacgggcgct gtcgacagtg cgcttggcca
      541 tgccggtcag cacgccgccg gggccgagtt cggcaaagga cgagacgccg agaccggcga
      601 gcgactgcag acattgcttc cagcgcaccg ggctggagag ttgggccgag agcaggctgg
      661 cccactcggt accggccgcg tgcggggcgg cgtcgacgtt ggacaccacc ggcacttcgg
      721 tgtcgcgcgg ctgggccgac gtgannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncgcc gagccccttg
      841 gcctcgttgc ccgccgcggc gacacctgcg ggcgacccgg cgatgacgac ctgtccgggg
      901 gcgttgaagt tggccaccca cacctcgctg tcggccagcc tgcaggcgac ttcgacctgg
      961 tcgtcgtcga ggccgagcac cgcggccatc gtgcccgggc tggccagacc ggcctcgtgc
     1021 atcgcgtcgg cccgctcgca caccagccgc acgccgtcgt cgaagctgag cgcgccgccc
     1081 gcggtgaggg ccgtgtactc gcccaagctg tgcccgnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnggcgtct gcgtcgagca gcagacgggc
     1261 gacgtcgcgc tcggcgacct gcgacgcttc ctcgaccagt tcccagctct catggtcgac
     1321 ccagggccga cccatgccgg gccgttgtga gccctggccc gggaacgtga acgcaagcat
     1381 gaaccgtgac aatacgccgc gagttcgggt cgagtcctac taccggctgg tagctggtcc
     1441 ctcgtcgtga ttcttggccg tttttcgcgg tgatcacgcc gtgtggcgag caggtgcctg
     1501 gatatgctgg cgcctccacc ggcccgagtg gcggaatggc agacgcggag gactcaaaat
     1561 cctttgtagg tgactacgtg tgggttcgag tcccacctcg ggcacaacgc cggtgggtcg
     1621 ggcttggagc agtgtggttt cagcgtgggg cgaggatgtc gtcgacgctg acggacaggc
     1681 ggcggcgcca gttggtcgcg ttgagcgtgc ccgggtggtt ggggcgttcg gtagacgcgg
     1741 tcatgtcgtc catcgacacc agtcggaccg ccgcgggtga agccaacagc gcggcgtgcg
     1801 cagctgcgat cgcgggtccg atcagggcat cgccatcgcc atcgccatcg ccatcggtgt
     1861 cggcatcggc atcggcgtcg tggccgacgt cgcccgggcg gggaagcacg aggtccggct
     1921 gcaccgtggc gatcgccgtg gcgagacggt cgcgggccac gacgttgccg tcgnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnngcg tccggctcga cggtgccgag gtcttcgccg
     2161 atcacgaacg ctccggcgcg ggtggcttcg aggcagacga tcgcgagcaa ctcgtcggcc
     2221 gggaactgca cgtacgcgcc gtcggccggg gtggcgtcga ccgggatcca gaactgacgg
     2281 aacagcccga tgacgtggtc gatgcgcaac gcctgcacac cctgcaggca cgcgcggatc
     2341 gtgtcgatga acggtcggta gccggcctcg cgcaggcgcc aggggacgaa cgggggaatg
     2401 ccccaggtct gacccgccgg ggtgaaggag tctggcggtg caccgaggca catgtcgagg
     2461 gcgagcaggt cttggaactg ccaggcgtcg gcgcccagcg gcgagaaccc gacggcgaga
     2521 tcggcgatga tcgcgacgcc ggtcgcggcg gcttccgcca actggtggtg ggcgaggcct
     2581 tggcaccacg cgtggaaact ggcggcgcgg gtcaggtccg cgctcgcgaa cgcggcggtc
     2641 aacgcctgtg gatcgggccg gtcgagcccg tcgggccaac tggaccaatc gggtccgtag
     2701 cggtcgcaca gtgcgttcca ggccgggacg aggtcgccgg acatgatcgc catcgccacg
     2761 tcgaagtcgg ccctcgccgc gcccgacccg atctcgatct cgaacagcgc ttccaaggca
     2821 gcccgcttgg cggtccaggc tgcgtcgcgg tcgatcagct cgtcggggcg gcaccacagc
     2881 tcgctggccg ggtcgccctc gatcgcgatt gcgagcgggc tgagatggcg ccgcgagctg
     2941 gggtagtagg ggctcggctc ttggggcaag ctcggcgcgg gttggtgcag cggactgatc
     3001 aacagagtcc gtccgcccgc ctgatgcagc gaggcgcaca acgtgcgcag gtcgcccagg
     3061 tcgccgatgc cccacgaccg ctcgctccac agcgacgcca gttgggtggc cgcgccccac
     3121 gcgcggggga tgtcgggaca gcgccccggg tgcacgatca gcgtcgtctc cgggccgccg
     3181 tcgaccggga tgaggcggtg gtaaccgagg ggtaggtcgg tgggcagtgc ggccacgatg
     3241 ccgaggtcga ggccgtcttc ggtgacgatg cggcactcgt tccacagcga tccggccgtg
     3301 ccttgcacca cgaaccacag cggtgggccg gggcgggcac cgccgatcgc ggcgcgcagc
     3361 ctggcccggg tctcgtcgct ggtcgggtgc cacgtgccgt cgatgtcgaa gtagccgtcg
     3421 ctgatacccc acgcatcgca gttcacgccg ttcagtctgg cccgcgaacg tcccgtccgt
     3481 agactcgccg ccatgcgtgc gtcgtgtcga gtctcacggg gagcgccatg tcgctgagct
     3541 cacggctggt cgaacgcaag ctgcgccaga gcgcgagcag gctgcgcgcc ctgcgggacg
     3601 agttgcgggt catcgacgag cagttgccgt cgctgcaggc cgatgccgac gatggcgaca
     3661 tgcgcgccct gttcgacgag accggtggat cgaacgccga agcccgccgg gcacggggca
     3721 gcgccgaggc gatggttcgc cagcgggccc gcgtggtcga gtcgatcgcg acctgggaac
     3781 gacgccagga cgaattgctc gaccgcctcg ctcgccgctg agaccggcgt gatcgtggtt
     3841 gaatgtggcg gccgcaacgg aaaggacctc ccgtgacgat tcgcgtggtg atcgccgagg
     3901 acgaagccat catccggctc gacctcaagg aaacgctcga ggaagagggc tacgacgtgg
     3961 tgggcgaaac cggccgcggc gacaaggcgg tcgaactggt ccgtgagctt cgccccgacc
     4021 ttgccatcct cgacatcaag atgccgggca tggacggcct ggaggcggcg gcgatcatca
     4081 acgccgagcg g
//
LOCUS       DAORNF010000255         4089 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_16374_length_4089_cov_1.601636, whole genome shotgun sequence.
ACCESSION   DAORNF010000255 DAORNF010000000
VERSION     DAORNF010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4089
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16374_length_4089_cov_1.601636"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="PLV68_10780"
     CDS             complement(<1..455)
                     /locus_tag="PLV68_10780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01774.1"
                     /translation="MQRFPRYEAWLAGEVAPTFKQLEDFARKTHTPLGFFFLEEPPVE
                     TVPIPDFRTIGDRQVGAAGAVVTADLLDVIYACERRQEWYRDNQLLEGEPPLPFVGVA
                     STVDAVGDVAGQMRQVLDWTAQSRDVRRTSDAALTWLREHAEAAGVLVM"
     assembly_gap    486..498
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(648..2057)
                     /locus_tag="PLV68_10785"
     CDS             complement(648..2057)
                     /locus_tag="PLV68_10785"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HRE01775.1"
                     /translation="MTELPKGWVSSRLADVADVRLGRQRSPKNATGDRMRPYLRAANV
                     KWAGLDLSDVNEMAFTAAESQTYELRPGDLLLGEASGSPSEVGKPGQYKGEIEGCCFQ
                     NTLLRVRLPEELLPDFYEHYFREQALNGRFAAGSRGVGIRHLGAAALSDWTIPVPPSA
                     EQERIVTAIEEAFSKLDAGEAGLRAVRQLLKRMRDAILTAAVTGRLVPQDPTDTPAAK
                     LLADLGLATAGDDEDTPESWARVRLGDIAHVGSGTTPRRGDASYWEDGSVPWVTSGLI
                     TNGVIREVREFVTERALQETSLRLWPMGTLLVAMYGEGQTRGRCAELGVEATCNQACA
                     AISLLDPLRVYKDFLRLSFDANYAANRRLASGGVQPNLNAGLIKDMVLSLPPPEQQSR
                     IVAEVDRQFSFVDACERAVDVGLARSAGLRRSVLKAAFEGRLVPQDPADESASVLLDR
                     IRAERAAQPVAKRRARQTA"
     gene            complement(2054..>3247)
                     /locus_tag="PLV68_10790"
     CDS             complement(2054..>3247)
                     /locus_tag="PLV68_10790"
                     /inference="COORDINATES: protein motif:HMM:NF020052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification enzyme R subunit
                     C-terminal domain-containing protein"
                     /protein_id="HRE01776.1"
                     /translation="GTDVRPLECVMFMLQVRSRNFFEQMKGRGVRVIDPNDLQAVSAD
                     ATAKTHFVIVDAVGVTEAEFNDTNPLDRRKADSLKKLLDQVAAGVRDADVVSAIAGRL
                     ARLDRVITPDDRTEIAKAAGGVGLADLVRFLTDAVDTDKAWEVAAAANGGADPTDEQL
                     HEARAGLVEKACRVLAERPELRTKLVEVRRSYTQVLDETSKDEIIDAGFSRDATDRAR
                     TTIESWQAFVAEHRVDIDALQILYSRPYGKRLTLRAIKELATAIGRPPYNWTPERLWA
                     AYEALEASRVRGSAGTQFTNLISLVRKALDPDRDLVAYPLTVDERFRNWLAQQTQAGT
                     TFTDDQLAWLTRIKDHLATSLTIAPDDFELEPFIGHGGYGRANTTFAGRLAPLLDELT
                     QELVA"
     assembly_gap    3248..3267
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3268..>3708)
                     /locus_tag="PLV68_10795"
     CDS             complement(<3268..>3708)
                     /locus_tag="PLV68_10795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit R"
                     /protein_id="HRE01777.1"
                     /translation="DFDVYRIRTKISEQGSTVEAGLVTKFRERETRKVRLEKLDEDVV
                     YDACQLDRDVVAVDQIRTVIRTFRDNLFKPLDEGGIFPGRSEVPKTLIFAKDDSHADD
                     IVRICREEFGRGNDFAVKITYRTTGVKPEDLLNQFRNSFNPRIAV"
     assembly_gap    3709..3721
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3722..4063)
                     /locus_tag="PLV68_10800"
     CDS             complement(<3722..4063)
                     /locus_tag="PLV68_10800"
                     /inference="COORDINATES: protein motif:HMM:NF037510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HRE01778.1"
                     /translation="MVITTVQRMYSMLRGEEELPEDVDEKSGAEIEPERPVEVAYNPA
                     IPIEAFDVVIVDECHRSIYGVWRQVIEYFDAFLVGLTATPAKQTFGFFNQNLVVEYGH
                     AEAVADGVNVDF"
BASE COUNT          659 a         1312 c         1359 g          713 t
ORIGIN      
        1 atcatcacca gcacccccgc tgcctcggcg tgctcgcgca accaggtcaa cgccgcatcg
       61 gaggtccggc gcacgtcgcg gctctgcgcc gtccagtcga gcacctgtcg catctggccg
      121 gcgacgtcac caacggcatc aacggtgctc gccacgccga cgaacggaag cggcggttca
      181 ccttcgagga gctggttgtc gcggtaccac tcctggcgcc gctcacatgc atagatcacg
      241 tcgagcaggt ccgcagtcac caccgcaccc gccgctccga cttggcgatc accgatcgta
      301 cggaagtcgg ggatcgggac cgtctcgacc ggaggctcct ccaagaagaa gaaccccagc
      361 ggtgtatgcg tcttgcgagc gaagtcctcc agttgtttga acgtgggcgc cacctcgccg
      421 gcgagccacg cctcgtagcg gggaaaccgc tgcacccaga cctcgtcgtc gatcccggat
      481 cgagcnnnnn nnnnnnnnga gcccattcga ggacacgctg tggcacgttg acccgcaagt
      541 gacggtcttt gcccgtccgc tcaaggacat ccaagctcac gacagctcac ctccacgatg
      601 cgacacgccg tccacggtcg acggtacagg gaggatgtga ccacgattca tgccgtttgc
      661 ctcgcccgcc gcttggcgac cggctgcgcc gcgcgctcag ccctgatccg atccagcagc
      721 accgacgccg actcatccgc cggatcctgc ggcacgagcc gcccctcgaa cgccgccttc
      781 aacaccgacc gccgcaagcc agccgaccga gccagcccga catcgaccgc tcgctcgcac
      841 gcgtccacga aggagaactg ccgatcgacc tcggcgacga tccgagactg ctgctcgggc
      901 ggcggcagtg acagcaccat gtccttgatg agcccagcgt tgaggttcgg ttggacgcca
      961 cctgaagcga gtcgacggtt agccgcatag ttcgcgtcga acgacagcct caggaagtcc
     1021 ttgtacacgc ggagcgggtc caggagtgag atcgctgcgc acgcctggtt gcacgtggcc
     1081 tcgacaccca gctctgcgca acggcccctc gtctgtccct ctccgtacat cgcgacgaga
     1141 agcgtgccca ttggccacaa ccgaagggac gtctcctgga gcgctcgttc ggtaacgaac
     1201 tcacggactt cccggatgac cccgttggtg atcagcccgc tcgtcaccca cgggaccgag
     1261 ccatcctccc aatagcttgc atccccccgc cgtggggtgg tgccggaccc cacgtgcgct
     1321 atgtcgccaa gacgtacccg ggcccaactc tcgggagtgt cttcgtcgtc gccagcagtg
     1381 gcaaggccaa ggtcggcgag gagcttggcg gcgggggtgt cggtggggtc ttgggggacg
     1441 aggcggccgg tgacggcggc ggtgaggatg gcgtcccgca tgcgcttgag gagttggcgg
     1501 acggcgcgga gtccggcttc gccggcgtcg agcttggaga acgcttcctc gatcgccgtg
     1561 acgatccgct cctgctcggc agagggtggc actgggatcg tccagtccga cagcgccgca
     1621 gcaccgagat gccggattcc gacgccccga gaacccgcag caaaccgtcc gttcagcgct
     1681 tgttcacgga agtagtgctc gtagaagtcg gggagcagct cctccgggag acggacgcga
     1741 aggagcgtgt tctggaagca gcacccctcg atctcgccct tgtactgccc cggcttgccg
     1801 acctcggagg ggctgcccga ggcttcaccc agcagcagat caccaggccg aagctcgtag
     1861 gtctgggact cggcagctgt gaacgccatc tcgttgacat cagacaagtc gagcccggcc
     1921 cacttgacgt tcgcagcgcg taggtagggg cgcatccgat caccggtcgc gttctttgga
     1981 gacctctgtc gaccaagtcg aacgtcggcg acatcagcga gccgcgagga gacccagccc
     2041 ttggggagtt cggtcatgcg acgagttcct gggtgagttc gtcgaggagg ggggcgagtc
     2101 ggccggcgaa ggtggtgttg gcgcggccgt agccgccgtg gccgatgaag ggttcgagtt
     2161 cgaagtcgtc gggggcgatg gtgaggctgg tggcgaggtg gtccttgatg cgggtcagcc
     2221 acgcgagttg gtcgtcggtg aaggtggtgc cggcctgggt ctgttgggcg agccagttcc
     2281 gaaatcgttc gtcgacggtg agtgggtagg caaccaggtc gcggtcgggg tcgagggctt
     2341 tgcggacgag gctgatcagg ttcgtgaact gagtgcccgc ggagccgcgg acgcgtgagg
     2401 cttcgagtgc ttcgtaggcg gcccagagcc gttcgggggt ccagttgtac gggggccgcc
     2461 cgatggcagt ggcgagttcc ttgatcgccc tgagggtgag ccgtttgccg taggggcggg
     2521 agtagaggat ctggagggcg tcgatgtcga cgcggtgttc ggcgacgaac gcctgccagg
     2581 actcgatggt ggtgcgggcg cggtcagtgg cgtcgcggga gaacccggcg tcgatgatct
     2641 cgtccttgga ggtctcgtcg agcacctggg tgtagctgcg tcgcacctcg accagcttcg
     2701 tgcgcaactc cggacgctcc gcgagcaccc ggcaggcctt ctcgaccagc ccggcgcgtg
     2761 cctcgtggag ttgttcgtcg gtggggtcgg cgccgccgtt ggccgccgca gcgacctccc
     2821 aggccttgtc ggtgtcgacg gcgtcggtga ggaatcgcac aaggtccgcc aggccgacac
     2881 cgccggcggc tttggcgatc tcggttcggt catcgggggt gatgacccgg tcgaggcggg
     2941 ccagacgccc ggcgatcgcc gacaccacgt ccgcgtcgcg aacgcccgcg gcgacttggt
     3001 cgagcagctt cttcagcgag tcggccttgc gacgatcgag cgggttggtg tcgttgaact
     3061 ctgcctcggt gaccccgacg gcgtcgacga tcacgaagtg ggtcttcgca gtcgcgtcgg
     3121 cggacactgc ttggaggtcg ttcgggtcga tcacccgcac gccgcggccc ttcatctgct
     3181 cgaagaagtt ccgggaacgg acctgcagca tgaacatgac acactccaac ggtcgcacat
     3241 cggtgccnnn nnnnnnnnnn nnnnnnngac tgcgatgcgc gggttgaagc tgttgcggaa
     3301 ttggttcagc aggtcctcgg gcttcacgcc cgtggtgcgg taggtgatct tcaccgcgaa
     3361 gtcgttgccg cggccgaact cctcccggca gatccgaacg atgtcatcgg cgtggctgtc
     3421 atccttggcg aagatcagcg tcttgggaac ctcgctgcgg ccggggaaga ttccgccctc
     3481 gtcgagcggc ttgaacaggt tgtcgcggaa cgtgcggatc acggtgcgga tctgatcgac
     3541 ggcgacgacg tcacggtcga gttggcaggc gtcgtagacg acgtcttcgt cgagcttctc
     3601 gaggcgcacc ttgcgggtct cgcgctcacg aaacttcgtc accaacccgg cctcgacagt
     3661 cgacccctgc tcgctgatct tggtgcggat ccggtagaca tcgaagtcnn nnnnnnnnnn
     3721 ngaagtcgac gttcacgccg tcggcaactg cttcggcgtg gccgtactcg acgaccaggt
     3781 tctggttgaa gaacccgaac gtctgcttcg ccggtgtcgc cgtcaaaccg accaggaacg
     3841 cgtcgaagta ttcgatcacc tgacgccaca ccccgtagat ggaacggtgg cactcgtcga
     3901 cgatcaccac gtcgaacgcc tcgatcggga tcgccgggtt gtacgccacc tccaccgggc
     3961 gttccggttc gatctccgca ccggacttct cgtcgacgtc ctcgggcagc tcttcctcgc
     4021 cgcgcagcat cgagtacatg cgctgcactg tcgtgatcac cacgcgtgcc gccggatcga
     4081 tcctgttcg
//
LOCUS       DAORNF010000256         4088 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_16379_length_4088_cov_1.918175, whole genome shotgun sequence.
ACCESSION   DAORNF010000256 DAORNF010000000
VERSION     DAORNF010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4088
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16379_length_4088_cov_1.918175"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    401..420
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            493..930
                     /locus_tag="PLV68_10805"
     CDS             493..930
                     /locus_tag="PLV68_10805"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HRE01779.1"
                     /translation="MDIHGIAKRQMDDAGHMYTRARRLIIDTLTTLNAPATIPTILQT
                     APTLVQSSLYRNLAVLQDVGLVTRVDVGDDRSYYELSEAVTDDHHHHLVCRVCRSVTD
                     VALPTRAERALDKAFSEAAGVVGFELKEHRVDLVGVCADCQAA"
     gene            complement(976..1593)
                     /locus_tag="PLV68_10810"
     CDS             complement(976..1593)
                     /locus_tag="PLV68_10810"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HRE01780.1"
                     /translation="MTATNPIDSTDAYPDLDALEALFRSRRSSVVCEREREIPAGLLQ
                     RLLASMSSAPNHRRTVPWRLALFTGAGRVALGDALAADRIAAAAPEQLDEAVIHKTRT
                     KYGRAPAVVLVGCAASDDPIRHREDLFACAAGIEHLLLAATAAGLVSLWSSPPVLYGE
                     QTSRLAGFDPTTSFVGAVYLGHRREGSDPLPPRDPIDLPIAAITG"
     gene            complement(1590..>2861)
                     /locus_tag="PLV68_10815"
     CDS             complement(1590..>2861)
                     /locus_tag="PLV68_10815"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRE01781.1"
                     /translation="RGRRPPRLLVSGRAGRTVASLRQRNFRLFFVGQLLSSVGTWLHQ
                     VSETWLVYKLTGNGFAMGAVVLARFVPVLVLGMWGGAIADRFDKRKTLYITQSVRGGS
                     ALVLGIWTLVGEVHVVTVLALAFTAGLANAIDNPVRRAFISELVDDDLVLNAVSLNST
                     VMATSRVVGPLLAGALIATVGVGWSFTINGISYVAMIASLAAMDASRFRARRRRNADS
                     KATVADGLRYICRTRELWVPLAMVGVVSAFAWNWEILLAVMATDVFGGGARLYTVMFA
                     VLSVGTFFGALFNAGQATIRARQLVTLAGGLGVAMAATSMVPWLALLFVGLLCSGIGA
                     AMFNTASNAVVQVTGRGEYHGRVMAVFTTLFVGTKGIGGTIAGGVMQRWGSRWAIAVC
                     GLGCLAAAAIGALGLDDRIPKTERHSLQEAT"
     assembly_gap    2862..2886
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2887..>3754)
                     /locus_tag="PLV68_10820"
     CDS             complement(<2887..>3754)
                     /locus_tag="PLV68_10820"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HRE01782.1"
                     /translation="GGNRTAIKLDYGRHLRAAHERAIARGQDLHIVVCLGTDISLMYA
                     AAFMGSQMPESADELAAAGGIQGSPLQMARAVTSDLLVPASTEIVLEARISPTETVHE
                     GPFAEFVGYESDDGPAPVMTVDAVTHRHNPIYFAINGAGRETVMLRKYVLEASALTAM
                     RAAVPIVSDVELTAGGLYRFHILIQVAKKSRQHDGLQRNAVLAAFAALKDLDAAIVVD
                     DDVDLRDATQVEWAVATRFEASRDILLIPAARGHEYVRVSDRGQRAKFAMDATVPFDD
                     RPRFSRAPFSEPV"
     assembly_gap    3755..3838
                     /estimated_length=84
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3839..>4088)
                     /locus_tag="PLV68_10825"
     CDS             complement(<3839..>4088)
                     /locus_tag="PLV68_10825"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HRE01783.1"
                     /translation="GDAALLFERVSGSSMPVLGNFLASPANVEAAFGLDRDGIRAAMT
                     RAISAPIAPETVTDPACQQVVIRHSETPIDLGSLLPALH"
BASE COUNT          699 a         1393 c         1297 g          570 t
ORIGIN      
        1 gtcagcacca ccgggccagt caccgtgttc gccgcatcaa ccaccacccc cggccagcga
       61 cgacgaacca gcccgaaacc agcgaacacc tcgacacacc gctgcccggg caacgccgcg
      121 ttcaaccggc gctgctgtgg caccaccacc gacggtgcca cacgaatgag accgtccaag
      181 cacacccggc cggtcagagc ggtcgggccg gttgctctgg tcgtcgcatg caacccaccc
      241 ccggccggag acgacgaacc acaccgaaac gagtgaacgg tcggacagta ccctgcccac
      301 gcattgctgc gtcaggtctg gcggccgggc ggtcacgtgg ctggcgacgc cgccaaggcc
      361 catgccaccg accggcaacc ccgacggctt gagccggtcc nnnnnnnnnn nnnnnnnnnn
      421 ccgacgtcgc gagtcggcac gagctggggc tgtgcaggga tacggtgagg attctcaggt
      481 attctcattt tcatggatat ccacggaatc gccaagcggc aaatggacga cgcggggcac
      541 atgtacaccc gggcccggcg gttgatcatt gacacgctca cgacgctgaa cgctccggcg
      601 acgatcccga cgatcctcca gaccgcgcca accctggtgc agagctcgct ctaccgcaac
      661 ctggccgtgc tgcaagacgt cgggctggtc acgcgggtcg acgtcggcga cgaccgctcg
      721 tactacgagt tgagcgaggc cgtcaccgac gaccaccacc atcacttggt ctgtcgggtg
      781 tgccgttcgg tcaccgacgt cgcactgccc acccgggccg agcgtgcgct cgacaaggca
      841 ttcagcgaag cagctggcgt ggtcggtttc gaactgaagg agcaccgcgt cgatctcgtc
      901 ggcgtgtgcg ccgattgcca agccgcctga caccggcaag cccggccgcg cgtgcgccac
      961 cgctgctgcg ggtgttcagc cggtgatggc ggcgatcggc aagtcgatcg ggtcgcgcgg
     1021 cggcaacggg tcgctgccct cgcggcggtg gccgaggtag acagcaccga cgaagctggt
     1081 cgtcgggtcg aatccggcca gacggctggt ctgttcgccg tacagcaccg gcggtgacga
     1141 ccacaggctg accaggccgg ccgcggtcgc ggcgagcagc aagtgctcga taccggccgc
     1201 gcacgcgaac aggtcttcac gatgacggat cggatcgtcg ctggcggcac agccgaccaa
     1261 caccacggct ggcgcacgtc cgtacttcgt gcgggtcttg tggatcaccg cctcgtcgag
     1321 ctgttcgggc gcggccgcag cgatgcggtc tgcggcgagc gcgtcgccga gcgccacccg
     1381 tcccgcaccg gtgaacaggg ccagccgcca cggcacagtg cggcggtggt tgggcgcgga
     1441 actcatcgag gcgagcagcc gctgcaacag accggccggg atctcacgtt cgcgctcgca
     1501 caccacgctg ctgcgccgcg agcggaacaa cgcttcgagc gcgtccaagt cgggatatgc
     1561 gtccgtcgaa tcgatcggat tggtcgcggt catgtcgcct cctgcagtga gtgtcgctct
     1621 gtcttgggaa tgcggtcgtc gagaccgagg gccccgatgg cggccgcggc cagacagcca
     1681 aggccacata ccgcgatcgc ccaccggcta ccccagcgtt gcatgacgcc accggcgatg
     1741 gtgccaccga tgcccttggt gcccacgaag agggtcgtga acacggccat cacccggcca
     1801 tggtattcac cacgtccggt cacctgcacg acggcgttgc tggcggtgtt gaacatcgcc
     1861 gcaccgatgc cgctgcacag cagcccgacg aacaacagcg ccaaccacgg caccatcgac
     1921 gtcgcggcca tcgcgacgcc gagtccgcca gcaagcgtca ccagttgccg cgctcggatc
     1981 gttgcctggc ccgcgttgaa cagggcgccg aagaacgtac cgacgctgag cacggcgaac
     2041 atcaccgtgt acagccgcgc tcccccgccg aacacgtcgg tcgccatcac cgcgaggagg
     2101 atctcccagt tccacgcgaa cgcgctgacg acgccgacca tcgccagcgg cacccacagc
     2161 tctcgggtgc ggcagatgta gcgcagcccg tcggcgaccg tcgccttcga gtcggcgttg
     2221 cgacgtcgcc gagcgcggaa tcgggacgcg tccatcgcag ccagcgatgc gatcatcgcc
     2281 acgtacgaga tgccgttgat cgtgaacgac cagccgactc cgacggtggc gatcagcgca
     2341 ccggccagca acgggccgac gacacgggac gtcgccatga ccgtcgagtt cagcgagacc
     2401 gcgttgagta ccaggtcgtc gtcgaccaac tcgctgatga acgcccgccg caccgggttg
     2461 tcgatcgcat tcgccaaccc ggcggtgaac gccagagcga gcacggtcac cacgtgcacc
     2521 tcgccgacca gggtccagat accgagcacc aacgccgacc cgccgcgcac gctctgggtg
     2581 atgtacagcg tcttgcgctt gtcgaagcga tcggcgatcg ccccgcccca catccccaac
     2641 acgagcacgg gcacgaagcg ggcaagaacg accgcaccca tcgcgaagcc gttgccggtg
     2701 agcttgtaca ccagccaggt ttccgacacc tggtgcagcc aggttccgac cgacgagagc
     2761 agttgtccga cgaagaacaa ccggaagttg cgttggcgaa gcgaggcgac cgtgcgcccg
     2821 gcccgcccgc tcacgagaag tcgcggcggg cgccggcctc gnnnnnnnnn nnnnnnnnnn
     2881 nnnnnncacc ggctcgctga acggtgctcg cgagaaccgt ggccgatcat cgaacggcac
     2941 ggtcgcgtcc atcgcgaact tggcccgttg accgcgatcg gaaacgcgga cgtattcgtg
     3001 accgcgcgcg gccgggatca gcaggatgtc gcgagacgct tcgaagcgcg tcgccaccgc
     3061 ccactcgacc tgggtcgcgt cgcgcaggtc gacgtcgtcg tccaccacga tcgccgcgtc
     3121 gagatccttc agcgcggcga atgcggcgag caccgcgttg cgttgcagcc cgtcgtgttg
     3181 gcggctcttc ttggcgacct ggatcaggat gtggaagcga tagagcccgc ccgcggtcaa
     3241 ctcgacgtcg ctgacgatcg gcacagcggc gcgcatcgcc gtgagcgccg acgcctccag
     3301 cacgtacttg cgcaacatca ccgtctctcg ccccgcgccg ttgatggcga agtagatcgg
     3361 gttgtggcga tgggtgaccg cgtcgacggt catcaccgga gccggaccgt cgtcgctctc
     3421 gtagccgacg aactcggcga aggggccttc gtgcaccgtc tcggtcggac tgatccgcgc
     3481 ctccaacacg atctcggtcg acgccggcac cagcaggtcg ctggtgacgg ctcgcgccat
     3541 ctgcaacggt gaaccttgga tgccaccggc ggcagcgagc tcgtctgcgc tctcgggcat
     3601 ctgcgaaccc atgaacgcag ccgcgtacat cagcgagatg tcggtgccga ggcagaccac
     3661 gatgtgcagg tcttggccgc gagcgatcgc ccgctcgtgt gcggcgcgca gatggcggcc
     3721 gtagtcgagc ttgatcgcgg tgcggttgcc cccgnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngg
     3841 tgcagtgcgg gcaacaacga gccgagatcg atcggcgtct cactgtgacg gatcacgacc
     3901 tgctggcacg ccgggtcggt cacggtctcc ggcgcgatcg gtgccgagat ggcacgggtc
     3961 atcgcggccc ggatgccgtc gcgatcgagg ccgaaggcgg cttcgacgtt ggccggactg
     4021 gccaagaagt tgccgagcac cggcatcgac gagccgctca cccgttcgaa caacagcgcc
     4081 gcgtcacc
//
LOCUS       DAORNF010000257         4065 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_16490_length_4065_cov_1.895761, whole genome shotgun sequence.
ACCESSION   DAORNF010000257 DAORNF010000000
VERSION     DAORNF010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4065
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16490_length_4065_cov_1.895761"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="PLV68_10830"
     CDS             <1..379
                     /locus_tag="PLV68_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRE01784.1"
                     /translation="PTRSVGDTHPLWTYAHVPHAYEGDATEAIIAQIERFAPGFRERI
                     VGMAVRSTTEMATYNPNYVGGDIAGGAASPMQTVFRPRVALDPYATGIPGTYLCSAST
                     PPGAGAHGMCGANAAARALRWLR"
     gene            complement(410..>672)
                     /locus_tag="PLV68_10835"
     CDS             complement(410..>672)
                     /locus_tag="PLV68_10835"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRE01785.1"
                     /translation="VMLSMPTATQDDPYLWRLLVDRLHQRRGIGTAVLDLLVAMCRER
                     GDSTLLVSWAPGRGSPEPMYLAYGFVPTGEIEGDEIVAAFPL"
     assembly_gap    673..691
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<692..1476)
                     /locus_tag="PLV68_10840"
     CDS             complement(<692..1476)
                     /locus_tag="PLV68_10840"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HRE01786.1"
                     /translation="MGADADMDTGATVGLPFEPIRTERLLLRPMRESDADAFFERRNH
                     PDVARYQTWTLPYPRERAERVVASVIEMGRPVADDTWMLTVADLDDTEVYGDLVLELK
                     WGTRTAEIGYTLHPRWWARGFALEASEALVAYAFETLDVTRVQGMLHPDNHASAMVLE
                     RLGLIFEGHTRSSFWVGDECSDDWIYGMTRADWDAWRTRARTRPELVELVELESDTVV
                     DAAKLVTHKSQERFVAPVLYSLAQALVPEVDEGVAVVPWYRGI"
     gene            complement(1606..>2299)
                     /locus_tag="PLV68_10845"
     CDS             complement(1606..>2299)
                     /locus_tag="PLV68_10845"
                     /inference="COORDINATES: protein motif:HMM:NF014177.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0032775 - DNA methylation on adenine
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HRE01787.1"
                     /translation="FAGSPLHPILLTSLIVAADKVDSTTGVQMAYLKQWAPRSYADLQ
                     LMMPELIAGAGHTVRGDAHALAGSLGDFDLAYLDPPYNNHRYFTNYHIFETLVTWDRP
                     DHYGVACKRTDSRSETTRSVFNAKRSMPSALAATIAALRAGVVIVSYNNESWLSLDDL
                     RDMCRVHGHVEVLAFDSKRYIGAQIGIHNPAGQRVGQVSHLHNLEYVVIAGPRATVTR
                     MAAAFPTAHLST"
     assembly_gap    2300..2309
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2310..2739)
                     /locus_tag="PLV68_10850"
     CDS             complement(<2310..2739)
                     /locus_tag="PLV68_10850"
                     /inference="COORDINATES: protein motif:HMM:NF014177.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0032775 - DNA methylation on adenine
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HRE01788.1"
                     /translation="MQRRYRRPSMIKYLGSKRRLVPVLGRLLDESGAQTALDLFTGTT
                     RVAQEFKRHGAVVTAVDLASYSDVFARCWIGTDAGAIDTSEHGELHAAIGELNALEGT
                     PGYVTETFCHASRFFQPKNGARIDAIRDAIARKPKGHDFVI"
     gene            3197..>3571
                     /locus_tag="PLV68_10855"
     CDS             3197..>3571
                     /locus_tag="PLV68_10855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01789.1"
                     /translation="MASQQTINLNLAGATAVLPANATAALINLTVDFDATMPSYLTVW
                     PQGEPRPLASANNAEPGLISANSMLAKLGTTGGISIFNQQGAVNLVIDVVGYTIALAD
                     AGLDHGGHLLSGNGAPSNSLGDD"
     assembly_gap    3572..3653
                     /estimated_length=82
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3804..>4065
                     /locus_tag="PLV68_10860"
     CDS             3804..>4065
                     /locus_tag="PLV68_10860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01790.1"
                     /translation="MDGEDGKSVTTGTTDPADADGADGDTYVNLTTGVVFVKVGGTWT
                     PLPGSLRGLLGGGSRYSSGSISLGVGVASNASVPVTFNNVGPQ"
BASE COUNT          668 a         1284 c         1347 g          655 t
ORIGIN      
        1 cccgacgcgc tcggtcggtg acacacaccc gctgtggacg tatgcgcatg ttccccatgc
       61 ttacgagggt gatgcgaccg aggcgatcat cgcccagatc gagcgctttg cgccgggatt
      121 ccgcgaacgc atcgtcggta tggcggtgcg gtcgaccacg gagatggcga cgtacaaccc
      181 gaactacgtc ggcggcgaca tcgcaggcgg cgcggcctca ccgatgcaga cggtgttccg
      241 cccgcgggtc gcgctcgacc cctatgccac cggcatcccc ggcacatacc tgtgctcggc
      301 atcgacgccg cccggcgccg gagcccacgg catgtgcggt gccaacgccg ccgcccgggc
      361 cctccgctgg ctgcgctagc caccccgccc gaactcaggt gagtctgggt cagaggggga
      421 aggcggcgac gatttcgtcg ccttcgatct cgccggtggg gacgaagccg taggcgagat
      481 acatcggttc gggtgagcct cggcctggcg cccacgagac cagcagggtc gagtcgccgc
      541 gctcgcggca catggccacc agcaggtcga gcaccgccgt accgataccg cggcgctggt
      601 gcaaacggtc gacgagcagg cgccacaggt aggggtcgtc ctgtgttgcg gtgggcatcg
      661 acagcatgac gannnnnnnn nnnnnnnnnn ntgaatgccg cggtaccacg gcacgaccgc
      721 cacgccctcg tccacctcgg gcaccagcgc ctgtgccaac gagtacagca cgggggcgac
      781 gaagcgttcc tgcgacttgt gggtgaccag cttggccgca tcgaccaccg tgtcgctctc
      841 gagctcgacc aactcgacga gttcggggcg agtgcgcgcc cgagtgcgcc acgcgtccca
      901 gtcggcccgc gtcatgccgt agatccagtc gtccgagcac tcgtcgccga cccagaacga
      961 cgagcgggtg tgcccttcga agatcagacc gaggcgctcc aacaccatcg ccgacgcgtg
     1021 gttgtcgggg tgcagcatgc cctgcacgcg cgtgacgtcg agcgtctcga acgcatacgc
     1081 gaccagtgcc tccgacgcct cgagcgcgaa gccacgggcc caccatcgcg gatgcagggt
     1141 gtagccgatc tcggccgtac gggttcccca cttcaactcc agcaccaggt cgccgtagac
     1201 ctcggtgtcg tccaggtcgg ccaccgtcag catccaggtg tcgtcggcga ccggtctgcc
     1261 catctcgatc accgacgcga ccacgcgctc ggcccgctcc cgtggatacg gcagcgtcca
     1321 ggtctgatag cgggcaacgt cggggtggtt gcgtcgctcg aagaacgcgt cggcgtcgga
     1381 ctcgcgcatc ggccgcagca acaaccgctc cgtccggatc ggttcgaacg gaagccccac
     1441 cgttgcaccc gtgtccatgt ccgcgtcagc ccccatcgcc ccagtcttgc ccagaacagc
     1501 ccagcccaac ctgtaccccg cccgcatgcc tggggcaggg ggaatcggat caggtggggc
     1561 gaactgcgtg gcccgaatta tatagaccga attgtgtagc ccgaattatg tggagaggtg
     1621 ggcggtgggg aaggcggcgg ccattcgggt gacggtggca cgggggccag cgatgacgac
     1681 gtactccagg ttgtgcaggt gcgagacttg tccgacccgc tgtccggccg ggttgtggat
     1741 gccgatctgg gcgccgatgt agcgcttcga atcgaatgcc aggacctcga cgtgaccgtg
     1801 cacgcggcac atgtcgcgca ggtcgtcgag gctgagccac gactcgttgt tgtacgacac
     1861 gatcacgacc ccggcccgta gcgcggcgat ggtggctgcc aacgcacttg gcatcgaccg
     1921 cttcgcgttg aacacgctgc gcgtcgtctc gctgcggctg tcggtgcgct tgcaggcgac
     1981 gccgtagtgg tcgggtcggt cccacgtcac cagcgtttcg aagatgtggt agttggtgaa
     2041 gtatcggtgg ttgttgtagg gcgggtcgag gtaggccagg tcgaagtcgc cgagtgagcc
     2101 ggcaagggca tgtgcatcgc cgcgcaccgt gtggccggca cccgcgatca actcgggcat
     2161 catcagttgc aggtcggcgt acgaacgggg tgcccattgc ttcagatagg ccatctgcac
     2221 gcccgtggtc gagtcgacct tgtcggcggc gacgatcaag ctcgtcaaca ggatggggtg
     2281 cagcggcgag ccggcgaacn nnnnnnnnnc gatgacgaag tcatggccct tgggcttgcg
     2341 ggcgatcgcg tcgcggatgg cgtcgatgcg cgccccgttc ttcggttgga agaaccgcga
     2401 cgcgtggcag aaggtttcgg tgacgtagcc cggcgttccc tccaacgcgt tcaactcgcc
     2461 gatcgcggcg tggagctcgc cgtgctcgga cgtgtcgatc gcgcccgcgt cggtgccgat
     2521 ccaacagcgc gcgaacacat cggagtagct ggccaggtcg accgcggtca ccacggcgcc
     2581 gtgtcgcttg aactcctggg cgacccgtgt ggttcccgtg aacagatcaa gcgcggtctg
     2641 cgcccccgac tcgtcgagca gacgaccgag cactggcacg agtcgccgtt tcgaaccgag
     2701 gtacttgatc atgctggggc gacggtagcg gcgctgcacg agatcgatcg ccgtacagca
     2761 agtcggacga cctcgctgtt ggccgcagga gcgggtattc gaccgctcgt atctgcacga
     2821 acagtgtcaa ttgttgattg agattgcagc gtgtcgtgcc gttgaaccct gtggcgtgct
     2881 ccgtgaccgg tggcacgcgt cgagaaaggg agagtcaatg cacgtcaacc tgttcaggac
     2941 gaggaagttc acacggaggc ccctcaccat gcggatgcgt cacgtcggac ggatcaccac
     3001 ggtcatcggg ctgaccgtgg cctgtggcct tggtgccgtc ggagccagtg cagcgccggg
     3061 accgcgagcc gcatcgacgg ccgcggccga ggcaacggct ggggagatgt cagttttcgt
     3121 accgatgtcc ccggtgcgag tgctggacac gcgcccggca ccgaatggtc cgatcggcgt
     3181 ccctacggca gcgccgatgg cgagccagca gacgatcaat ctcaaccttg ccggcgctac
     3241 cgcggtgttg ccggccaacg ctacagccgc gctgatcaac ctgaccgtcg acttcgacgc
     3301 gacgatgccg agctacctca ccgtatggcc gcagggcgag ccacggccgt tggcgtcggc
     3361 caacaacgcc gagcccggtc tgatctcggc caactcgatg ctcgccaagc tgggcaccac
     3421 cggcgggatc agcatcttca accaacaggg tgcggtcaac ctggtgatcg acgtcgtggg
     3481 ctacacgatc gctctcgccg atgccggtct cgaccatggc ggccatctgc tcagcggcaa
     3541 cggtgcaccg tccaattcgc tcggcgacga cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnccgggtg
     3661 cggatgggat cgatggtgtt gacggcgttg acggcgtcga tggcgttgat ggtgtcgacg
     3721 gcaccaacgg caccaacggt acgaacggta cgaatggtgt cgacggtgtt gacggcgttg
     3781 acggtacgga tggtgtcgac ggtgtggacg gcgaagacgg caagagcgtg acgaccggga
     3841 cgaccgatcc agccgatgcc gacggagccg acggcgacac gtacgtcaac ctgacgaccg
     3901 gcgtcgtgtt cgtcaaggtc gggggcacgt ggaccccgct accaggatcg ctgcgcggtc
     3961 tgctgggtgg tgggtcgcgc tactcgagcg gctcgatcag cctcggtgtc ggcgtggcga
     4021 gcaacgcatc cgttccggtc accttcaaca acgtcggacc ccagt
//
LOCUS       DAORNF010000258         4061 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_16511_length_4061_cov_1.608337, whole genome shotgun sequence.
ACCESSION   DAORNF010000258 DAORNF010000000
VERSION     DAORNF010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4061
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16511_length_4061_cov_1.608337"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="PLV68_10865"
     CDS             complement(<1..229)
                     /locus_tag="PLV68_10865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01791.1"
                     /translation="MFVGLVAPQDVVIAAHTLKDAGWQMCEALGLLHAAVEVTRRADR
                     SGLTPNVLGATLLRVQREVEALQTVVATLAFD"
     assembly_gap    321..334
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    625..711
                     /estimated_length=87
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1007..1103
                     /estimated_length=97
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1104..>1399
                     /locus_tag="PLV68_10870"
     CDS             <1104..>1399
                     /locus_tag="PLV68_10870"
                     /inference="COORDINATES: protein motif:HMM:NF025761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HRE01792.1"
                     /translation="SDGQNIDRAWPFDIIPRVIPGAEWDRISAGLAQRLRALNMFIDD
                     LYNDQRVIADGVFPAELLAASVNYREQCRGVRPKHGVWAHISGTDLVRDDDGVM"
     assembly_gap    1400..1571
                     /estimated_length=172
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1590..2431
                     /locus_tag="PLV68_10875"
                     /pseudo
     CDS             1590..2431
                     /locus_tag="PLV68_10875"
                     /inference="COORDINATES: protein motif:HMM:NF025761.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
     gene            2447..3400
                     /locus_tag="PLV68_10880"
     CDS             2447..3400
                     /locus_tag="PLV68_10880"
                     /inference="COORDINATES: protein motif:HMM:NF016086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HRE01793.1"
                     /translation="MALLARVADRLYWAARYLERAEDTARIIRAYHDLVVDFPGDVML
                     RWEPLAAICGNAVTGNADVTGDHTGDDDSGELAVLQFLIADRLNPGSVASCVSSCREN
                     LRTTREVIPRESWLTINQLSHYVASAAPGAVRRQSRDRFLARVIDDSRGVDGVLDSTM
                     TRASPYRLFRLGRLLERADMTTRVLGVRAAALLQMEQSGVEPLSEEVQWMGVLRSLSA
                     LQMYQRATKGPIDGEAVVRFLLYYATFPRSVQGCLDEIRSVLVALPTPHDVITALDGA
                     QKVLLDAQPMATDGHELDDAMDRVQIAIAGLDAAIAARFFH"
     gene            3472..>3752
                     /locus_tag="PLV68_10885"
     CDS             3472..>3752
                     /locus_tag="PLV68_10885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01794.1"
                     /translation="MPPGPGLQPSGPLREVLLDGSRLAERQERADRLLEADGAGHLVH
                     DLPVRGDGRVASIESRPWRVDPIPVVLDAQTFRWLSRAVIERMRALDVV"
     assembly_gap    3753..3766
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3803..>4061
                     /locus_tag="PLV68_10890"
     CDS             3803..>4061
                     /locus_tag="PLV68_10890"
                     /inference="COORDINATES: protein motif:HMM:NF016090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HRE01795.1"
                     /translation="MLAATPRYRVNAVGATPLRWLTTYAVDVVLGADGVWSVVQDLTD
                     APPGIGYAMIDRSVMSRVYPELMGTSTVASIATYPRAIRRAL"
BASE COUNT          585 a         1233 c         1244 g          615 t
ORIGIN      
        1 cgtcgaacgc caacgtcgcc accaccgtct gcagggcttc gacctcacgc tgcacccgca
       61 acagcgtggc acccaacacg ttcggggtga gaccggaccg gtcagcgcgc cgcgtgacct
      121 ccaccgcggc gtgcaacaac cccaacgcct cgcacatctg ccagccagcg tctttcaggg
      181 tgtgcgccgc gatcaccaca tcttgcgggg cgaccagtcc gacgaacata cgagaaccat
      241 acacacgttc gtgttgaaat gcaacctcag caacatcaca aatccagatg cccaaagccc
      301 tccctatcgc tcaagcggat nnnnnnnnnn nnnnagccag cactaccacc tcgccgcgcg
      361 gttgcgacac agctgacgac ggcccttggc ttcgcccgtg ttcggaatgg aaccacggca
      421 atgagcgcgc ggcggccacc cccggtctcg tcccggaggg ctgcttgctc cgcatcgaca
      481 gcccgatgtc cactccgcca caacgtgtga agtaataagt caagttgtgt gtaacacatg
      541 acaaacagcc acgtactcct gccacgggtc gctgagcaac ttgccggcct tcgggccgag
      601 caggaaagaa ggaccaggtg ttctnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncggcgactc
      721 agctgtgggg ggcgtcagtg tcggctggac gtatgtgtcc gcgatgtgcg cggagatgcg
      781 agcgatccct tcgtgcatca cgtcgggcac cgaaccgacg cgcatcaacc acagcatttg
      841 ttcgatggtt tggatggtgt tgcggggtgg ttacggaggt gtgaactgag gcgacgcggc
      901 tgctgagggt caatcggagt gggtgtatcg tccgcgctcg atggacgagc tcgtggggga
      961 tgacggcacg gcacggccag ccgccgcggc cctgatgtcg ctcatcnnnn nnnnnnnnnn
     1021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnactccga cggccagaac atcgaccgtg cctggccgtt
     1141 cgacatcatc ccgcgggtga tccccggcgc cgagtgggac cgaatcagcg ccggtctcgc
     1201 ccagcgcttg cgtgcgctga acatgttcat cgacgacctc tacaacgacc agcgtgtgat
     1261 cgccgacggc gtgttcccgg cggaactgtt ggcagcgtcg gtgaactatc gcgagcagtg
     1321 ccggggcgtg cgacccaagc acggcgtgtg ggcccatatc tccggcaccg atctggtgcg
     1381 cgacgacgac ggggtgatgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn natcgccccc cagctggtgg tgctcactcc gggcatcttc aactcggcct
     1621 acttcgagca ctcgttcctc gcccagcggt tgggcgccga gttggtggaa ggtgccgact
     1681 tggtggtcgg tgacgacgac tgcgtgtaca tgcgcacgat cgccggacgc gagcgagtgc
     1741 acgtgatcta ccgccgcatc gacgacatgt tcctcgatcc cgaggcgttc cggcccgact
     1801 cgatgctggg tgtgcccggc ctgatgcggg cgtggaaggc gggcaacgtc gggctcgcca
     1861 acgccccggg cgcgggggtc gccgacgaca aggtcgtcta cgcgtgggtc cccgacttga
     1921 tccgctacta cctcggtcag gaaccgctga tcgccaacgt ggcgacctat cgtacgtcat
     1981 cgagcacatc gaggacctcg tggtgaagcc ggccaacgag agcggtggct acggcctgct
     2041 catcggcgac cgggccacgc gcagcgaact cgacgcgcac atcgcgctca tcgaggccga
     2101 cccgcgcaac tgggtggcgc aaccgatcgt gtcgctgtcg accgtgccga cgttggtgcc
     2161 tgacggcgat ggccatgccg tcgaaccgcg ccacgtcgac ctccgtccgt tcgtgctcac
     2221 cggggcgacg agctacgtca cgccgggcgg gttgacccgc gtcgcgctgc cgaagggttc
     2281 gctggtggtc aactcgtcac agggtggcgg cagcaaggac acgtgggtgg tcgacggtgt
     2341 gttcgagccg accgcccggc ggtgcgtcac catcaccgcc cagccccgag cccaggccca
     2401 gtctcaggcg cagtcgcagg cgcagtcata ggagcgggtg aacgagatgg cgctgctggc
     2461 aagggtggca gaccggttgt actgggcggc gcggtacctc gagcgtgcgg aggacacggc
     2521 ccggatcatc cgcgcctacc acgatttggt ggtcgatttc cccggcgacg tgatgctgcg
     2581 ttgggagccg ctggcggcca tctgcggcaa cgccgtcact gggaatgccg acgtgacggg
     2641 cgaccacacc ggtgacgacg actcgggcga gctcgcggtg ctgcagttcc tgatcgccga
     2701 tcggctcaat cccggcagcg tcgcgagttg cgtgtcgtcg tgccgtgaga acctgcgcac
     2761 gacgcgcgag gtgatcccgc gcgagtcgtg gttgacgatc aaccagctgt cgcactacgt
     2821 cgccagcgcg gccccgggcg ctgttcggcg ccagtcgcgc gatcggttcc tggcccgggt
     2881 catcgacgac agccgcggcg tcgacggcgt gctcgactcg accatgacgc gcgctagccc
     2941 ctaccggctg ttccggctgg gccgtctgct cgaacgtgcc gacatgacca cacgcgtgct
     3001 cggcgtgcgt gccgccgcct tgttgcagat ggaacaatcc ggggtcgaac cgctcagcga
     3061 ggaagtgcag tggatgggtg tgctccgctc gctgtcggcg ttgcagatgt atcagcgcgc
     3121 caccaagggg ccgatcgacg gtgaagcggt cgtccggttc ctgctctact acgccacctt
     3181 tccgcggtcg gtgcagggct gcctcgacga gatccgctcg gtcctggtgg cgctgcccac
     3241 cccgcacgac gtcatcactg ccttggacgg agcccagaag gtactgctcg atgcccagcc
     3301 gatggccacc gacggtcacg agctcgacga cgccatggac cgcgtccaga tcgcgatcgc
     3361 gggcctcgac gcagcgatcg cagcccggtt cttccactga agtcagaccg ctctggcgcg
     3421 tgtgatggcc ctcttgtacg tgtgacgcga ggatcgggca agatggatgg catgcccccc
     3481 ggccccggat tgcagccatc gggaccgctg cgcgaggtgt tgctcgacgg ttcccgcctg
     3541 gccgagcgcc aggagcgggc cgatcgcctg ctcgaggccg acggtgccgg tcacctggtg
     3601 cacgacctgc cggtgcgcgg cgacggccga gtggcctcga tcgagagccg cccgtggcgc
     3661 gtcgacccga tcccggtggt gctcgacgcg cagacgttcc ggtggctgtc gcgggcggtg
     3721 atcgagcgga tgcgcgccct cgacgtggtg atnnnnnnnn nnnnnncgca cgctcgtagg
     3781 cgaccgcacg gttccggccg aggtgctggc ggcgacgccg cgctatcggg tcaacgccgt
     3841 tggcgccacg ccgttgcgct ggctcacgac gtacgcggtc gacgtcgtcc tcggggccga
     3901 cggcgtgtgg tcggtggtgc aagacctcac cgacgcgccg ccgggcatcg gctacgccat
     3961 gatcgatcgc tcggtgatgt cgcgcgtgta tcccgagctg atgggcacca gcaccgtcgc
     4021 ctcgatcgcg acctacccgc gtgcgatccg gcgtgccctt g
//
LOCUS       DAORNF010000259         4054 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_16546_length_4054_cov_1.570893, whole genome shotgun sequence.
ACCESSION   DAORNF010000259 DAORNF010000000
VERSION     DAORNF010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4054
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16546_length_4054_cov_1.570893"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(105..1766)
                     /locus_tag="PLV68_10895"
     CDS             complement(105..1766)
                     /locus_tag="PLV68_10895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01796.1"
                     /translation="MTDTNPPNPIDSADLPIAAAVRAWADELAATVPPYVPHSLTVTQ
                     TRPLHTRRRMGIGIAAGVLIVAGFTAIAWMRLTRNASTDIYHAPSSGSVTAEPTTAQT
                     TNSADPASTTSETITSTSAEPETTTTTTTTTEVPVAVDPTPVDGSNTVDGIEVMVRVP
                     GEVVAGTRAAIEVTVTNHRSEPIYWQAGGCATPAEVTVGPISTAGLAANSGGPGRLAM
                     EQLWDGNPDSLAPAIRTAAALGTRQLANIAEFAGWGSYGCTSDSNMQPIPSGGQVTDS
                     LSAEVRVPPGPLADAGRWDVTATFVGYADWEGYPNQPLATVSAHVVFAVVDHPDRGEV
                     DLDTLGAVIAADGRLADWYATTLIPGRPDLDQRHSIGLTWWRGAWELWVSPYWGSGDT
                     ARVRLDPATLAVTDVRIVRSGTPPDDEPDLDRDNSFLDQFPDRVLALGELASTSGATA
                     SPASASFGDTITITPHGDFVCDGMARFYRVNADGTTAEAGVSMTDQGNTTWNTPPEGS
                     PITVPPCRPVVASTVQVDIPQLAPGDYLVCLTDNSTTCAPVTITP"
     gene            complement(1759..2220)
                     /locus_tag="PLV68_10900"
     CDS             complement(1759..2220)
                     /locus_tag="PLV68_10900"
                     /inference="COORDINATES: protein motif:HMM:NF016432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRE01797.1"
                     /translation="MDHRERVGDFEAFVGRVEPGLRRALAGHMDHHVVSDALSEAFTY
                     AWEHWQTVRSLENPGGYLFRVAQSKSRTRRQGLADSPAPMAMPSVEPGLAAGMRALSP
                     QQRSVVWLVHGCQWTYRETAEALAISPTAVGTHLQRALTRLRQHLGVTADD"
     gene            complement(2335..2718)
                     /locus_tag="PLV68_10905"
     CDS             complement(2335..2718)
                     /locus_tag="PLV68_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03618 family F420-dependent PPOX class
                     oxidoreductase"
                     /protein_id="HRE01798.1"
                     /translation="MDPGNPGDDIVGFLTESHLATLTTLRRDASPHVVAVGFTYSPAE
                     RLARVITFASSVKAGNAARGGRAAVCQVDGGRWLTLEGTVRLTTDPAAIATAVEMYTA
                     RYRAPGERPDRVAIEIDVDRILGHA"
     assembly_gap    2721..2787
                     /estimated_length=67
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2788..3277)
                     /locus_tag="PLV68_10910"
     CDS             complement(<2788..3277)
                     /locus_tag="PLV68_10910"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HRE01799.1"
                     /translation="MATRPRSDLLLGEWACLGLLATGRSHGFAVATQLKPSSDLGRVW
                     SQSRPLVYRALDHLTELGYVTPTGEEPGIAGGNRTIYAVTPLGRRRLRTWLTTPVAHL
                     RDVRSELVMKVMLAERCRVDLRPMLAAQRAQFAALAESLAGQRASGDVVATWRSEFAD
                     AAI"
     assembly_gap    3418..3427
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3428..>3678)
                     /locus_tag="PLV68_10915"
     CDS             complement(<3428..>3678)
                     /locus_tag="PLV68_10915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01800.1"
                     /translation="TSTGLRVVVAGAAPGPAFAVIRPQAISLARRAPVGSSVRNTWPG
                     LVATIDRFGDRFGDRCRVSVDLQSGSGDASVSLVAEITV"
     assembly_gap    3679..3802
                     /estimated_length=124
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3803..>4054)
                     /locus_tag="PLV68_10920"
     CDS             complement(<3803..>4054)
                     /locus_tag="PLV68_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRE01801.1"
                     /translation="AGHLRHRPRELSGGQAQRVALARALAVDPRMLLLDEPLSALDVA
                     TRSAVRRDLRRHLAGFGGVRILVTHDPMDAYALADRVAI"
BASE COUNT          552 a         1250 c         1467 g          584 t
ORIGIN      
        1 attgctgaag gcctcgcttc gggcggaggg cgggaggtca ccgccgacgt catcgctgaa
       61 cttcgtgggc gtgcgctcgg cgcggacgcg tgagacaggt gaggctatgg ggtgatcgtc
      121 acgggggcgc aggtcgtcga gttgtcggtg aggcagacca ggtagtcgcc gggtgcgagt
      181 tgggggatgt cgacctgcac ggtgctggcc acgaccggcc tgcacggggg gacagtgatc
      241 ggtgatcctt ctggcggggt gttccaggtg gtgttgccct gatcggtcat gctcactccc
      301 gcttcggcag tcgtgccgtc cgcgttgacc cggtagaagc gggccatccc gtcgcatacg
      361 aagtcgccgt ggggcgtgat ggtgatcgtg tcgccgaaac tggcgctggc cgggctggca
      421 gtcgcgccgc tggtggaggc caactcgccg agtgccagca cccgatcggg gaactggtcc
      481 agaaacgaat tgtcacggtc gaggtctggt tcgtcgtcgg gcggggtgcc ggaccgaacg
      541 atccggacgt cggtgacggc gagggtggcc gggtcgaggc ggacgcgggc ggtgtcaccg
      601 ctgccccagt acgggctgac ccacaactcc cacgcaccgc gccaccacgt cagcccgatc
      661 gagtgccgct gatcgaggtc gggccgcccg gggatcaacg tcgtcgcgta ccagtccgcg
      721 agccggccgt ctgcggcgat cactgcgcca agggtgtcga ggtcgacctc gccgcgatcg
      781 ggatggtcga ccacggcgaa caccacgtgc gcgctgaccg tggccaacgg ttggttcggg
      841 tatccctccc agtctgcgta gccgacgaat gtcgcggtga cgtcccacct cccggcgtcg
      901 gcgagtgggc cgggcggaac acggacctcg gccgagagcg agtcggtgac ctgaccgccc
      961 gacggaatcg gttgcatgtt gctgtcgctg gtgcagccgt acgaacccca cccagcgaac
     1021 tcggcgatgt tggcgagttg gcgggtaccg agggcggcgg cggtgcggat cgctggggcg
     1081 agcgaatcgg ggttgccgtc ccacaactgc tccatcgcca ggcgtcccgg ccccccggag
     1141 ttcgcggcga gtccggcggt gctgatcggg ccgaccgtga cctcggcggg ggtcgcacag
     1201 ccgcctgctt gccagtagat cggttcgctg cggtggttgg tgacggtgac ctcgatcgcg
     1261 gcacgggtgc ccgccacgac ctcgccgggt acccgcacca tgacttcgat cccgtcgacg
     1321 gtgttggacc cgtcgaccgg cgtggggtcg acggcgacgg gaacctccgt cgtggtcgtg
     1381 gtcgtggtcg tggtctcggg ctccgcggag gtggacgtga tcgtttcgct ggtggtggac
     1441 gccgggtcgg cgctgttcgt cgtctgtgcc gtggtcggct cggcggtgac gctgccggac
     1501 gacggcgcgt gatatatgtc ggtactggcg ttgcgcgtga gacgcatcca ggcaatggcg
     1561 gtgaacccag cgacgatcag cacccccgcg gcgatgccga tgcccatccg gcgtcgggtg
     1621 tgcagtgggc gcgtctgcgt cacggtcagt gagtggggca cgtacggcgg caccgtcgct
     1681 gccagctcgt cggcccacgc ccgcaccgcc gccgcgatcg gcaggtcggc cgagtcgatc
     1741 gggttgggtg ggttggtgtc agtcatctgc ggtcactccc aggtgttggc gcaggcgcgt
     1801 gagcgcccgc tgcaggtgcg tgccaaccgc ggtcggcgag atcgccagcg cctccgctgt
     1861 ctcgcggtag gtccactggc agccgtggac cagccacacc accgagcgct gttgcggtga
     1921 cagggcgcgc atcccggccg cgaggcccgg ctcgaccgac ggcatcgcca tgggcgcggg
     1981 cgagtcggcg agaccctggc gacgagtgcg cgacttcgac tgggcgaccc ggaacaggta
     2041 gccgcccgga ttctcgaggc tgcgcaccgt ctgccagtgc tcccacgcgt acgtgaacgc
     2101 ctccgacagt gcatccgata cgacgtggtg atccatgtgc ccggccaggg cgcggcgcag
     2161 ccccggctcc acccgcccga cgaacgcctc gaagtcgcct acccgctcac ggtgatccat
     2221 agctcaccca gattgaccca cgaacacccc gatcgcgcca cacggctcgt tgtcgttccc
     2281 gcgagtacga gatcggtttc cgcccaagcg ctggaaaagg cgttgccgag ggggtcaggc
     2341 gtggccgagg atccggtcga cgtcgatttc gatggcgacg cggtcggggc gctcgccggg
     2401 ggcgcggtag cgggcggtgt acatctcgac cgcggtggcg atggcggcgg ggtcggtggt
     2461 gagccggacg gtgccttcga gtgtcagcca acgtccgccg tcgacctggc acaccgcggc
     2521 gcggccgccg cgggcggcat tgcccgcctt gaccgaactg gcgaaggtga tcacccgcgc
     2581 cagccgttcg gcgggggagt aggtgaagcc gaccgcgacc acgtgcggac tggcgtcgcg
     2641 gcgcagggtg gtgagtgtcg cgaggtggct ctcggtgagg aagccgacga tgtcgtcgcc
     2701 aggattgccg gggtcgatca nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnngga tcgccgcgtc ggcgaactcg ctgcgccatg
     2821 tggcgacgac atccccgctc gcgcgctggc cggcgagcga ctcggccagt gccgcgaact
     2881 gggcgcgctg ggcggccagc atcgggcgca gatcgactcg gcagcgctct gcaagcatca
     2941 ccttcatcac caactcgctg cgcacatcgc ggagatgagc gactggggtg gtcagccacg
     3001 tccgcaggcg cctgcggccc agcggggtga cggcatagat ggtgcggtta cctccggcga
     3061 tgccgggctc ttcacctgtc ggggtgacgt agcccaactc ggtgaggtgg tcgagggcgc
     3121 ggtagacgag cggtcgcgac tgggaccaga cgcgaccgag atcgctggac ggcttcagtt
     3181 gggtcgcgac cgcgaagccg tggctgcgac cggtggccaa caggccgaga caggcccact
     3241 cgccgaggag caggtcggag cgcggccgcg ttgccatgac gcccatggtg gcatgcgcgc
     3301 ccacgagtgg ggtgctttgc gctcccggcg acggctcaga ccggataggt gtcgatgtcg
     3361 gtggccttgg ccgaggcgac gatctcgtcg ccgggtcgca agccgagctc gtcgagtnnn
     3421 nnnnnnnaac cgtgatctcg gccacgagcg acacggaagc gtcaccgctg cccgattgca
     3481 ggtcgacgct gacccggcag cgatcgccga accggtcgcc gaagcggtcg atcgtggcga
     3541 ccaggcccgg ccacgtgttg cgcacgctcg agccgaccgg cgcacgacgg gcgagcgaga
     3601 tggcctgggg ccggatgacc gcgaaggccg gaccgggtgc cgccccggcg acgacgaccc
     3661 gcaggccggt gctggtcgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnagatggcg acccgatcgg cgagtgcgta cgcgtccatc
     3841 gggtcgtggg tgaccaggat gcggacaccg ccgaagccgg ccaggtgacg gcgcaggtcg
     3901 cggcgcacgg cgctgcgagt ggccacgtcg agcgccgaca acggctcgtc caacagcagc
     3961 atccgcggat cgacggcgag cgcccgggcc agggccacgc gttgggcctg accgccggag
     4021 agctcgcggg ggcggtgacg aaggtggccg gcca
//
LOCUS       DAORNF010000260         4042 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_16618_length_4042_cov_1.756208, whole genome shotgun sequence.
ACCESSION   DAORNF010000260 DAORNF010000000
VERSION     DAORNF010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4042
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16618_length_4042_cov_1.756208"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    244..253
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <254..748
                     /locus_tag="PLV68_10925"
     CDS             <254..748
                     /locus_tag="PLV68_10925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRE01802.1"
                     /translation="DVARDARLALIDGELAGWVLVNCRRTDDVQERAFVDGTVLPSAR
                     GRGVGRALFDWAIPEARQRLKAIDNDLPAFIRVYAAEQIETLARLLQRFGFAPERWFE
                     ELVRPLAERPNPPTPVGLDIVPWDAPRRVAAAAQPRVRRPLGFGAGGRVRVGRAGQRL
                     RRPH"
     gene            755..1051
                     /locus_tag="PLV68_10930"
     CDS             755..1051
                     /locus_tag="PLV68_10930"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRE01803.1"
                     /translation="MGRDRWRRRGRRVRAQRALRGRRSGHRATRRADRRRDWRGRGVA
                     SALIARSMDGFEQAGFTHALLNVDSASPTGASRLYRSLGFEPIARTVVHQIAVR"
     assembly_gap    1235..1373
                     /estimated_length=139
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1374..>1648
                     /locus_tag="PLV68_10935"
     CDS             <1374..>1648
                     /locus_tag="PLV68_10935"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRE01804.1"
                     /translation="EQDVAVAPDTIWRIYSMTKPVTSVAAMMLWEEGLFELHDPVAKF
                     IPEFADVKVWRGGSVTRPDLEPLLRPMEMWHLFTHTAGLTYGFMYNH"
     assembly_gap    1649..1669
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1670..>2308
                     /locus_tag="PLV68_10940"
     CDS             <1670..>2308
                     /locus_tag="PLV68_10940"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRE01805.1"
                     /translation="EWGVPPNTDLAGICSILAKQPLLFQPGAEWSYSMAIDVLGRVVE
                     VITGQSLGEFMQERLFGPLGMVDTAFWAPADKASRVAVLYGAHPVTGQAFPLGAMGDG
                     AVTEPSAHLGGGGLVSTTSDYLHFAEMLRARGSFGGHRLLSPRTVDYMTSNHLPGNAD
                     LTEFGRPLFSETTFDGVGFGLLGSVTLDPVTAKVPGSVGDFGWGGAASTSFWV"
     assembly_gap    2309..2374
                     /estimated_length=66
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2413..>2595)
                     /locus_tag="PLV68_10945"
     CDS             complement(2413..>2595)
                     /locus_tag="PLV68_10945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01806.1"
                     /translation="NLFRLQRLAPGTQEVTQFTSTVRHQRASGESGGATCRDSPTAPP
                     LNGATNQVVMVPGVSR"
     assembly_gap    2596..2799
                     /estimated_length=204
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2800..3351
                     /locus_tag="PLV68_10950"
     CDS             <2800..3351
                     /locus_tag="PLV68_10950"
                     /inference="COORDINATES: protein motif:HMM:NF037627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01807.1"
                     /translation="SGEAPEPVERVIEAPDALVRLIHAMDPAPAYVLGPRWEIVAWNK
                     AEEHLYPLLHDLPADDRNLLWLMFCEPAARSLIADWEDRARSSLAEFRADTAQLLADP
                     DVAELIRRLTAASAEFARWWAELDVAAFQTRLRHYHHPRAGELVFEYQQLVPSEWPLL
                     RVICQLPLPGDDSAQRLSNWRDI"
     gene            3398..>3692
                     /locus_tag="PLV68_10955"
     CDS             3398..>3692
                     /locus_tag="PLV68_10955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="catalyzes the reduction of mycothione or
                     glutathione to mycothione or glutathione disulfide;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="mycothione reductase"
                     /protein_id="HRE01808.1"
                     /translation="MGDMPQHFDVVIIGTGSGNSIPTADYDNKRIAIVERGVFGGTCL
                     NRGCIPTKMFVYTADVAQMVGHASTYGVDAEITGVRWPEIVARVFGRIDPIPPN"
     assembly_gap    3693..3702
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3703..>4042
                     /locus_tag="PLV68_10960"
     CDS             <3703..>4042
                     /locus_tag="PLV68_10960"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRE01809.1"
                     /translation="AYRESLANTTVFTGSGRFVGPKQVEVAAADGSTTRITGDDIVIA
                     TGARPLIPAIDGLDQVEYHTSDTVMRVAELPRRLIVLGGGYIATEMAHVFASLGSEVT
                     IVNRSARLLAR"
BASE COUNT          569 a         1205 c         1218 g          600 t
ORIGIN      
        1 gttgcagacc cttgtggcca tcgccgattc gggatgcccg ctcccacgct gtaaatctgg
       61 tcacacggtg cgggtcgggt cggtactgtc acggcacatg ggggatggtg tgacgctggt
      121 tggggcaaca ctggacgatt gcgatcggat caccgcgctg atcaaccggg ctgaggagtt
      181 cgacggtgtc gagcgcgtac tcgacaccga cgagatgcgc gaagaactgt cgagcgatca
      241 tctnnnnnnn nnngacgtgg ctcgcgacgc gcggctcgcg ttgatcgacg gcgagcttgc
      301 cggatgggtg ctggtcaact gccgtcgcac cgacgacgtg caggagcgag cgttcgtcga
      361 cggcacggtg ttgccctcgg cgcggggccg aggtgtggga cgcgcactgt tcgactgggc
      421 aatacccgag gctcgccaac ggttgaaggc gatcgacaac gacctgcccg cgttcatccg
      481 cgtctacgcg gccgagcaga tcgagacgtt ggcgcgtttg ctgcagcggt tcgggttcgc
      541 ccccgagcgc tggttcgaag agctcgtccg gcccttggcc gaacgcccca acccgccgac
      601 ccccgtcggg ctcgacatcg tgccgtggga cgcgccacga cgagttgcgg ctgctgcgca
      661 accacgcgtt cgccgaccat tggggttcgg tgccggcgga cgagtccgag tgggacgagc
      721 gggtcaacgg cttcggcggc cgcattgatc tgagctgggt cgcgatcgat ggcgacggcg
      781 tggccgtcgg gttcgcgctc aacgggcact tcgcggccga cgaagcggtc accgggcgac
      841 gcgacgggct gatcgacgcc gcgactggcg cggacgtggc gtcgcgtcgg cgttgatcgc
      901 ccgctcgatg gacggcttcg aacaggccgg tttcacccac gcgttgctca acgtcgactc
      961 ggccagcccg acaggtgcca gccggctcta ccggtcgctg ggcttcgagc cgatcgcccg
     1021 caccgtcgta caccagatcg ccgtgcgctg aatgggccgc cgattctggc gctgatccag
     1081 cacatggcgc aggctggatc gcgcagggct gcgcggtgca gggttcttcc ccgctcgccg
     1141 cccgcggcta caacaggttc gacgtctcat caccccacgc cgaggagcat ccatggatct
     1201 gaccacgccc gccatcgatc cggccgacgc ggggnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncgagcag
     1381 gacgtcgcgg tagctccgga caccatctgg cgcatctact cgatgaccaa gccggtcacg
     1441 tcggtcgcgg cgatgatgct gtgggaggag ggcttgttcg aactgcacga tccggtcgcg
     1501 aagttcatcc ccgagttcgc cgacgtgaag gtgtggcgag gcgggtcggt cacccgtccc
     1561 gatctcgagc cgttgctgcg cccgatggag atgtggcacc tgttcaccca caccgctggg
     1621 ctcacgtacg ggttcatgta caaccatcnn nnnnnnnnnn nnnnnnnnnc gaatggggtg
     1681 tgccgcccaa caccgatctg gccggaatct gcagcatcct ggccaagcaa ccgctgctct
     1741 tccagccagg cgccgagtgg agctactcga tggcgatcga cgtgctcggc cgcgtggtcg
     1801 aggtgatcac cggtcagagt ctgggcgagt tcatgcaaga gcgcctcttc gggccgctcg
     1861 gcatggtcga caccgccttc tgggcgccgg ccgacaaggc cagccgcgtc gccgtgctgt
     1921 acggcgcaca tccggtgacc ggccaggcgt tcccactggg ggcgatgggt gacggcgcgg
     1981 tcaccgagcc gtcagcgcac ctgggcggtg gcgggttggt atcgacgacg agcgactacc
     2041 tccactttgc cgagatgctc cgcgcccgcg gctcgttcgg cggccatcgt ctcctgtcac
     2101 cccgcacggt cgactacatg accagcaacc acctgcccgg caatgccgac ctcaccgagt
     2161 tcggacggcc gctgttcagc gagaccacct tcgacggggt cggcttcggt ctgctcggct
     2221 cggtcacgct cgacccggtc accgccaagg tcccggggtc ggtgggtgac ttcggttggg
     2281 gtggtgcagc cagcacgtcg ttctgggtnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncatccg cagccagctc aagcagatgg
     2401 tcagccaagc catcatcgac tgacaccggg caccatcacc acctggttcg ttgccccgtt
     2461 cagcggtggc gccgtgggag aatcacgaca tgttgccccg cccgactcgc cgctggcccg
     2521 ctgatgcctg accgtcgagg tgaactgggt gacttcttgc gtgcccggcg cgagtcgttg
     2581 caaccggaac aggttnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna gcggcgaggc tccggagccg
     2821 gtcgagcggg tgatcgaggc ccccgacgct ctcgtgcggc tcatccatgc gatggaccct
     2881 gctccggcgt acgtgttggg gccacggtgg gagatcgtcg cctggaacaa agccgaggag
     2941 catctctacc cgctactcca cgacctaccc gccgacgatc gcaacctgct gtggttgatg
     3001 ttctgcgagc ctgccgcgcg cagcctgatc gccgattggg aagaccgggc gcgcagcagc
     3061 ctggccgagt tccgggccga cacggcccag ttgctggccg atcccgacgt ggccgaactg
     3121 attcgacggc tcacggcggc aagcgccgag ttcgcccgct ggtgggccga gctcgacgtc
     3181 gccgcgttcc agactcggct gcgccactac caccacccgc gggccggcga gctggtgttc
     3241 gaataccaac agctcgtccc gagcgagtgg ccactcctca gggtgatctg ccaactcccc
     3301 ctgcccggcg acgacagcgc ccaacgcctc tccaactggc gcgacatcta agtggttcag
     3361 ggctggtctt cagcgcggag ccaataccat cgggggtatg ggtgacatgc cacagcactt
     3421 cgacgtcgtg atcatcggga cgggatcggg caactcgatt ccgactgctg actacgacaa
     3481 caagcggatc gcgatcgtcg agcggggggt gttcggcggc acctgtctca accggggttg
     3541 catcccgacc aagatgttcg tctacacggc cgatgtcgcg cagatggtcg gccacgcgag
     3601 cacgtacggc gtcgacgccg agatcaccgg ggtgcgctgg ccggagatcg tggccagggt
     3661 gttcggccgc atcgacccca tcccaccgaa cgnnnnnnnn nnaggcctat cgggagagct
     3721 tggccaacac cacggtgttc accgggtcgg gacgtttcgt cggcccgaag caggtcgagg
     3781 tcgccgcagc ggatggctcg accacacgca tcaccggcga cgacatcgtg atcgccaccg
     3841 gggcgcgccc gctgatcccc gccatcgacg gcctcgatca ggtcgagtac cacaccagcg
     3901 acaccgtgat gcgggtggcg gaactgccgc ggcggctgat cgtgctcggc ggcggataca
     3961 tcgcgaccga gatggcgcac gtgttcgcca gcctgggcag tgaggtgacc atcgtcaacc
     4021 gcagcgcgcg cctgctcgcc cg
//
LOCUS       DAORNF010000261         4035 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_16668_length_4035_cov_1.751759, whole genome shotgun sequence.
ACCESSION   DAORNF010000261 DAORNF010000000
VERSION     DAORNF010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4035
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16668_length_4035_cov_1.751759"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..651)
                     /locus_tag="PLV68_10965"
     CDS             complement(<1..651)
                     /locus_tag="PLV68_10965"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRE01810.1"
                     /translation="MASSILSDLFSLTGKTALVTGGSRGIGAMIAQGLLEAGASIVIS
                     ARKAHELEAAREELSQFGSVQTVVADVSNLAGVRALAEATAAIFPSLSILVNNAGATW
                     GQPIDEFTEAGWDRTVDTNLKAPFFLMQALLPNLRAAGTADDPARVINIGSIDGLHVN
                     PLEAYAYGASKAGIHHMTRGLARRLGPEHITVNAIAPGPFESKMMAATLENFGDAIS"
     gene            682..>1709
                     /gene="hemE"
                     /locus_tag="PLV68_10970"
     CDS             682..>1709
                     /gene="hemE"
                     /locus_tag="PLV68_10970"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HRE01811.1"
                     /translation="MTVLAAGESAPAISPFLAAARGERAAHTPVWFMRQAGRSLPEYR
                     AVRGEGSILQAIKDPELSAAITMQPVTRYGVDAAVLYSDIVVPVHAIGFGVDVAPGTG
                     PVAADPFRSAADLARVRPLDPEADTPYVIATVAILARELSVPVLAFAGAPFTVASYLI
                     EGKPSRTYEHTKALMHTDETTWHELMDRLASHAIASIRSQLDAGAAAFQLFDSWAGAL
                     SRTDYERFALPHSRRVFAALASSHPHAPGIHFGIGCDHLLEPMYGAGARVMGLDWRTR
                     IADARRRLGPGTAIQGNLDPALVLAGWPVARTGCDAVLADNDGHPAHIFNLGHGVQPN
                     TDPGVLEQ"
     assembly_gap    1710..1852
                     /estimated_length=143
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1853..>2137
                     /locus_tag="PLV68_10975"
     CDS             <1853..>2137
                     /locus_tag="PLV68_10975"
                     /inference="COORDINATES: protein motif:HMM:NF012964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HRE01812.1"
                     /translation="RPPSDDQLADLLRRYDAIGGLSPLAERTEAQRVGLQAALDQIAP
                     GEFIVVLATKHATPSIEAAVDAIAADGIGRIVALVLAPHDSALSIGQYVDR"
     assembly_gap    2138..2196
                     /estimated_length=59
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2197..2682
                     /locus_tag="PLV68_10980"
     CDS             <2197..2682
                     /locus_tag="PLV68_10980"
                     /inference="COORDINATES: protein motif:HMM:NF012964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HRE01813.1"
                     /translation="TEPAFVSFLAHDLAARLAALPAPVRVVFTAHSLPARIVATGDPY
                     PDELSATATAVAAACGLAGDQWQIAWQSAGRTPEPWLGPDILEVIDAAGVDGTTRSVL
                     VSAVGFVADHLEVLYDLDVEAAARAASLGLAFDRTACVNDDPAVMAALAARVVATGTP
                     R"
     gene            2679..>3306
                     /gene="hemG"
                     /locus_tag="PLV68_10985"
     CDS             2679..>3306
                     /gene="hemG"
                     /locus_tag="PLV68_10985"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HRE01814.1"
                     /translation="MTTRVAVVGGGITGLTVALRVRQQWPDAVVTLYEAADRLGGKIA
                     ASPFAGLAAVDEGADAFLTRAPAAIGLARELGLGDSLTSPAVGNAAVWWHELHPIPDG
                     LMLGIPTGVAALTRSRLLTWSGKLRAGLEPILPATGTGADSVGLLVRKRFGRQVHERL
                     VDPLVGSIYAADTDRFSLAGVPQIAQLASHRSLLLAGRAARAKAATVVD"
     assembly_gap    3307..3316
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3317..>3636
                     /locus_tag="PLV68_10990"
     CDS             <3317..>3636
                     /locus_tag="PLV68_10990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRE01815.1"
                     /translation="PLPIFATPTAGMAALTGALGEAITALGVDIRLGTTVGELRRGNA
                     AGTGAVGSGRWMVDGEAYDAVVVASPAKPSAPLLAATSGDAAGLLAAYDHAGVVMVTL
                     AVPD"
     assembly_gap    3637..3800
                     /estimated_length=164
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3801..>4035
                     /locus_tag="PLV68_10995"
     CDS             <3801..>4035
                     /locus_tag="PLV68_10995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01816.1"
                     /translation="DALVHAAVDEVGRHVGTDLQPSAVRLGRWPMAFPQYRPGHAARV
                     DAIDRALRRDAPGVFVVGASYRGIGIPACIQQAN"
BASE COUNT          496 a         1344 c         1268 g          551 t
ORIGIN      
        1 cgagatcgca tcgccgaagt tctccaacgt ggccgccatc atcttgctct cgaacggccc
       61 gggcgcgatc gcgttgaccg tgatgtgttc ggggccgaga cggcgggcca agccgcgggt
      121 catgtggtgg atgccggcct tgctggctcc gtacgcgtac gcctccagcg gattgacgtg
      181 gaggccatcg atcgagccga tgttgatcac ccgggccgga tcgtcggcgg tgcccgcggc
      241 gcgcaggttg ggcagcaacg cctgcatcag gaagaacggg gccttgaggt tggtgtcgac
      301 cgtgcgatcc catccggcct cggtgaactc gtcgatcggc tggccccacg tcgcccccgc
      361 gttgttgacc agaatcgaca gcgacgggaa gatcgccgcg gtcgcctcgg ccagggcgcg
      421 gaccccggcg aggttgctga cgtcggcgac gacggtctgc accgagccga actgcgacag
      481 ctcctcgcgg gccgcttcca gctcgtgggc cttgcgcgca ctgatcacga tcgacgcccc
      541 cgcttcgagc agcccctggg cgatcatggc gccgatgcca cggctaccac cggtgacgag
      601 ggcggtcttg ccggtgaggg agaacagatc agacaggatg ctggaagcca tgaggggcga
      661 ttctacgtag gctctggtcc catgaccgtg ctcgccgcgg gtgagtccgc gcccgccatc
      721 agtccgtttc tcgctgccgc ccgtggggag cgggcagcgc acacaccggt ctggttcatg
      781 cgccaggctg gccgcagcct gcccgagtac cgcgccgtgc gcggcgaggg cagcatcctc
      841 caggcgatca aggaccccga gctctcggca gcgatcacga tgcaaccggt cacccggtac
      901 ggcgtcgatg cggcggtgct gtacagcgac atcgtcgtcc ccgtgcacgc gatcgggttc
      961 ggtgtcgacg tcgcaccggg caccggtccg gtcgccgccg atcccttccg ttcggccgcc
     1021 gacctggccc gggtccgccc actcgatccc gaggccgaca cgccgtacgt gatcgccacg
     1081 gtcgcgatcc tggcccgcga gctgtccgta ccggtcctgg ccttcgccgg cgcgccgttc
     1141 accgtcgcca gctacctgat cgagggcaag cccagccgca cctacgagca caccaaggcg
     1201 ttgatgcaca ccgacgagac gacctggcac gagttgatgg atcgcctcgc ctcacacgcg
     1261 atcgcctcga tccgcagcca gctcgacgcc ggcgcggcgg cgttccaact gttcgactcg
     1321 tgggccggtg cactcagccg gaccgactac gagcgcttcg ccctccccca ctcgcggcgg
     1381 gtgttcgcgg cgctggcatc gtcgcacccg catgcccccg gcatccactt cggcatcggc
     1441 tgcgaccacc tcctcgaacc gatgtacggc gccggagcgc gagtgatggg cctcgactgg
     1501 cgcacccgca tcgccgacgc ccggcgacgc ctcggccccg gcaccgcgat ccagggcaac
     1561 ctcgaccccg cactggtgct cgccgggtgg ccagtggccc ggaccgggtg cgacgcggtg
     1621 ctggccgaca acgatggcca tccggcgcac atcttcaacc tcggccacgg cgtgcagccc
     1681 aacaccgacc cgggcgtgtt ggagcagatn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncgcccgcc
     1861 gagcgatgat caactcgccg acctgctgcg ccgctacgac gcgatcggcg ggctgtcacc
     1921 gctggccgag cgcaccgagg cgcaacgggt cgggctgcag gccgcgctcg accagatcgc
     1981 gcccggcgag ttcatcgtcg tgctcgccac caagcacgcc acgccgagca tcgaagcagc
     2041 ggtcgatgcg atcgccgccg atgggatcgg ccgcatcgtc gcgctggtgc tcgcgccaca
     2101 cgacagcgca ctgtcgatcg gccagtacgt cgatcgcnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnaccg agccggcgtt cgtgtcgttc
     2221 ctcgctcacg atctcgcggc gcggttggct gcgctgcccg cgccggtgcg ggtggtgttc
     2281 accgctcact cgttgcccgc ccgcatcgtc gccacgggcg atccctatcc ggacgagttg
     2341 tcggccacgg ccaccgcggt cgccgcggcg tgcggccttg ccggcgacca gtggcagatc
     2401 gcctggcaat cggccggacg tacgcccgaa ccgtggctcg gccctgacat cctcgaggtg
     2461 atcgacgcgg ccggggtcga cggcacgacc cgctcggtct tggtgtcggc cgtcggcttc
     2521 gtcgccgacc acctcgaggt gctgtacgac ctggacgtcg aagccgcggc acgggctgca
     2581 tcgctgggcc tcgccttcga tcgcaccgcg tgcgtcaacg acgatccggc cgtcatggcc
     2641 gcattggccg cccgggtcgt cgccaccgga accccccgat gacgacgcgc gtcgcggtag
     2701 tcgggggtgg catcaccggg ttgacggtcg cgctgcgggt tcgccagcag tggccggatg
     2761 cggtggtgac gctgtatgag gcggccgatc ggctcggagg caagatcgcg gccagcccgt
     2821 tcgccgggct cgccgcggtc gacgaaggtg ccgacgcgtt cttgacccgt gccccggccg
     2881 cgatcggcct ggcccgcgag ctcggcctcg gcgattcgct gacctcacca gcggtgggca
     2941 acgcggccgt gtggtggcac gagttgcacc cgatccccga cgggctgatg ctcggcatcc
     3001 ccaccggtgt cgcggcgctc acgcgtagcc gccttctcac ctggtcgggc aagctgcgtg
     3061 ccgggctgga gccaatcctg cctgcgaccg gcacgggcgc cgactcggtc gggttgttgg
     3121 tgcgcaaacg cttcggccgc caggtgcacg agcggctcgt cgacccgctc gtcggcagca
     3181 tctatgccgc cgacaccgac cggttcagcc tggccggcgt accccagatc gcccagctcg
     3241 cgagccatcg cagcctgctg ctcgcgggcc gcgcggcgcg agccaaggcc gcaacggttg
     3301 tagacgnnnn nnnnnnaccg ttgccgatct tcgccacgcc caccgcgggc atggcggcgt
     3361 tgacgggggc gctcggcgag gcgatcaccg cgctcggcgt cgacatccgc ctcggcacga
     3421 cagtgggcga actgcggcgc ggcaacgctg ctggcaccgg cgcggtcggg tcgggccggt
     3481 ggatggtcga cggcgaggcc tacgacgcgg tcgtcgtcgc gtcgccggcc aagccgagcg
     3541 cgccgttgct ggccgccacg tcgggcgatg ccgccggatt gctcgccgca tacgaccacg
     3601 ccggcgtcgt gatggtgacg ctggcggtac ccgattnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn cgacgcgctg gtgcacgcgg ccgtcgacga ggtcggccgc
     3841 cacgtcggca ccgatctgca acccagtgcg gtgcgactcg ggcgttggcc gatggccttc
     3901 ccgcagtacc ggcccggcca cgcggccagg gtcgacgcca tcgaccgtgc gctgcggcgc
     3961 gacgcccccg gcgtgttcgt cgtcggcgcg agctatcggg gcatcggtat ccccgcctgc
     4021 atccagcagg ccaac
//
LOCUS       DAORNF010000262         4034 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_16678_length_4034_cov_1.137220, whole genome shotgun sequence.
ACCESSION   DAORNF010000262 DAORNF010000000
VERSION     DAORNF010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4034
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16678_length_4034_cov_1.137220"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>838)
                     /locus_tag="PLV68_11000"
     CDS             complement(<1..>838)
                     /locus_tag="PLV68_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HRE01817.1"
                     /translation="ERLGQVARTAVRYLDRVVDINFYPTEAAARSNARWRPVGLGVMG
                     LQDVFFQLRLPFDSPEAQTLSRQIAEEIYFHALWASTELAEQHGRHTAFAETRAAQGD
                     LQFDLAGAAPTDAARWQPLRDRIAAHGLRNSLMIAIAPTATIAHIAGAYECIEPQVSN
                     LFKRETLSGEFLQINGYLVRDLQARGLWTAEMIAAVKRAEGSIQQIDGIPGDLKQLYR
                     TAWEIPMRSLIDMAAARGVFIDQSQSLNLFVESPTIGKLSSMYLHAWKQGLKTTYYLR
                     SRP"
     assembly_gap    839..967
                     /estimated_length=129
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<968..>1227)
                     /locus_tag="PLV68_11005"
     CDS             complement(<968..>1227)
                     /locus_tag="PLV68_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HRE01818.1"
                     /translation="FMERVEKDWTWSLFDPKVVPHLTDLYGPEFEAAYVEAERQELYE
                     RQVPARQLYAQMMRTLAQTGNGWMTFKDPSNLRCNQTGGDRV"
     assembly_gap    1228..1396
                     /estimated_length=169
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1397..>1614)
                     /locus_tag="PLV68_11010"
     CDS             complement(<1397..>1614)
                     /locus_tag="PLV68_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01819.1"
                     /translation="HPQMSSCYLLDSPTDELDSIYDRYKDIARLSKFAGGIGVAFHRI
                     RSRGSLIAGTNGLSNGIVPFLKTLDASVT"
     assembly_gap    1615..1818
                     /estimated_length=204
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1819..2295)
                     /locus_tag="PLV68_11015"
     CDS             complement(<1819..2295)
                     /locus_tag="PLV68_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP cone domain-containing protein"
                     /protein_id="HRE01820.1"
                     /translation="MTLTRDPSTETLRPLLDDTGEIASPMRVRKRNGSLEPVDLNKIV
                     RAITRAAGRLDGIDPMRVATRTISGLCDGTTTTELDELSIRTAAALVSEEPNYSRLAA
                     RLLATVIGKEVANQDIHAFSQSIAAGHAAGLIGEPTAQLVAMHARKLNEAVDHKRDR"
     assembly_gap    2505..2625
                     /estimated_length=121
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2626..3816
                     /locus_tag="PLV68_11020"
     CDS             <2626..3816
                     /locus_tag="PLV68_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HRE01821.1"
                     /translation="PASTFRSVRIHQMHALRDRPYMHGAYGPRLRHVSYFLSHITRPC
                     FRAGTVDLAPNNFSEMRAILKGATNDPLVIATASPPDRHGFFSLGTSADYVSSFIGRA
                     RFFLEANPQMPRTFGRNQIHISQVAGWTQADSPLVEVQPATPGPADERIADLVAERIP
                     DRSTLQIGIGAIPNAILGRLSDHSDLGIHTELLSDGVIDLVEQGVVNGVAKQLNRTKT
                     VGTFALGTRRLYDFIDENAAFELWPVRYVNDPRVIAQENAFVSINATIAVDLLGQCAS
                     ETINGVYYSSSGGQADFARGAMYSPEGQGFVVLHSVTHGGRHGGTDGGTRADQISKIV
                     AQHAPGEVITTLKNTVDKVVTEWGVAELRGRSIRDRAHRLIAIAHPDHRDALRAEAAR
                     LGYV"
BASE COUNT          574 a         1158 c         1127 g          552 t
ORIGIN      
        1 ccgggcgcga gcgcaggtag tacgtggtct tgagaccctg cttccaagcg tgcaggtaca
       61 tcgacgacaa cttgccgatc gtcggcgact cgacgaacag gttcagcgac tggctctggt
      121 cgatgaacac gccacgggcc gcggccatgt cgatcagcga ccgcatcggg atctcccacg
      181 cggtgcggta cagctgcttg aggtcgccgg gaatgccgtc gatctgctgg atcgagccct
      241 cggcccgctt gaccgcggcg atcatctcgg cggtccacag cccccgcgct tgcaggtcgc
      301 gcacgaggta gccgttgatc tgcaagaact cgccggacag tgtctctcgc ttgaacaggt
      361 tgctgacctg cggctcgatg cactcgtagg ccccggcgat gtgggcgatg gtggcggtgg
      421 gcgcgatcgc gatcatcagc gagttgcgca gaccgtgagc ggcgatgcga tcgcgcagcg
      481 gctgccatcg cgccgcatcg gtgggcgcgg ccccggcgag gtcgaactgc aggtcgccct
      541 gcgccgcccg cgtctctgcg aacgcggtgt ggcgcccgtg ctgttcggcc aactcggtcg
      601 aggcccacag ggcgtggaag tagatctcct ccgcgatctg gcggctgagc gtctgtgcct
      661 cgggcgaatc gaacggcagg cgcagttgga agaacacgtc ctgcagaccc atcacaccca
      721 agccgaccgg acgccagcgg gcgttcgagc gcgctgcggc ctcggtggga tagaagttga
      781 tgtcgaccac gcggtcgaga tagcgcaccg cggtgcgggc cacctggccg aggcgctcnn
      841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      961 nnnnnnncac ccgatcgccg ccggtctggt tgcagcgcag gttggacggg tccttgaacg
     1021 tcatccagcc gttgccggtc tgggccaggg tgcgcatcat ctgcgcgtac aactggcggg
     1081 ccggaacctg gcgctcgtac aactcctggc gctcggcttc gacgtaggcg gcttcgaact
     1141 cgggtccgta caggtcggtg agatgcggca ccaccttggg gtcgaacagc gaccacgtcc
     1201 agtccttctc gacccgctcc atgaacannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnngtga ccgacgcatc gagcgtcttg aggaacggga cgatgccgtt
     1441 cgacaatccg ttggtgcccg cgatcaggct gccccgcgag cggatgcggt ggaatgcgac
     1501 gccgatgcca cccgcgaact tggacagccg ggcgatgtcc ttgtagcggt cgtagatcga
     1561 gtcgagctcg tcggttgggc tgtcgagcag gtagcacgac gacatctgcg ggtgnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnncc ggtcgcgctt gtggtcgacg gcctcgttga gcttgcgcgc
     1861 gtgcatcgcc accagttgcg ctgtgggctc gccgatcagc ccagccgcgt ggcccgcggc
     1921 gatcgactgg ctgaaggcgt ggatgtcctg gttggcgacc tccttgccga tcactgtggc
     1981 cagcaatcgg gccgcgaggc gggagtagtt cggctcctcg ctgaccaacg cggccgcggt
     2041 gcggatcgac agctcgtcca actcggtggt cgtggtgccg tcgcacagcc cactgatcgt
     2101 gcgcgtcgcg acccgcatcg ggtcgatgcc gtccaggcgc ccagcagccc gcgtgatcgc
     2161 ccgcacgatc ttgttgaggt cgaccggttc gaggctgccg ttgcgcttgc gcacccgcat
     2221 cgggctggcg atctcaccgg tgtcgtcgag cagtggacgc agggtctcgg tgctcgggtc
     2281 tcgggtcaaa gtcatcaggt cctccagggg ttccgccggg cgaggtaggg gtccaaacac
     2341 aagatgtggg gacagatctg acgccggacc caacatcttg aggggagtca gtattacaca
     2401 cctgtagtta cacgggcgca agtccgtcgt ctccgacgaa acggatatgc ccgcgagcag
     2461 gtgcccggcg accgggtacg agatgggcag aatgtgctgg tgagnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncccgc cagcacattc
     2641 cgatcggtgc gcatccacca gatgcacgcc ctccgcgacc gcccctacat gcacggcgcc
     2701 tacgggccgc gcctgcgcca cgtgtcgtac ttcctcagcc acatcacccg cccctgcttt
     2761 cgggccggca cggtcgacct ggcgccgaac aacttctccg agatgcgggc gatcctcaag
     2821 ggcgcgacca acgatccgct ggtgatcgcc acggcgagcc cgcccgatcg gcacggattc
     2881 ttcagcctgg gcacgtcggc cgactacgtg tcgtcgttca tcggccgggc ccggttcttc
     2941 ctcgaagcca acccgcagat gccgcgcacc ttcggccgca accagatcca catcagccag
     3001 gtcgccggat ggacccaggc cgactcgccg ctggtcgagg tgcaaccggc cacccccggc
     3061 ccggccgacg agcggatcgc cgatctggtc gccgaacgga ttcccgatcg ctcgacgctg
     3121 cagatcggca tcggtgccat ccccaacgcg atcctcggcc gcctcagcga ccacagcgac
     3181 ctcggcatcc acaccgagtt gctgtccgac ggcgtgatcg atctggtcga gcaaggcgtg
     3241 gtcaacggtg tcgccaaaca gctcaaccgc accaagaccg tcggcacctt cgccctcggc
     3301 acccgacggc tgtacgactt catcgacgag aacgctgcgt tcgaactgtg gccggtgcgc
     3361 tacgtcaacg acccgcgggt gatcgcgcag gagaacgcat tcgtgtcgat caacgcgacg
     3421 atcgctgtcg acctgctcgg ccagtgcgcg tcggagacga tcaacggcgt gtactactcc
     3481 tccagcggcg ggcaggccga cttcgcacgg ggcgcgatgt actcaccgga aggacaggga
     3541 ttcgtcgtgt tgcactccgt cacgcacggc gggcggcacg gcggcacgga cggtgggacg
     3601 cgagccgatc agatcagcaa gatcgtcgcc cagcatgcac cgggcgaggt gatcaccacc
     3661 ttgaagaaca cggtcgacaa ggtggtgacc gagtggggcg tggccgagtt gcgcggtcgc
     3721 tccatccgcg accgagcaca ccgcctgatc gcgatcgccc accccgatca ccgcgacgca
     3781 ctgcgcgccg aggccgcccg cctcgggtac gtctgacatc tcacgtgcag gcgcgacggg
     3841 ccaatcgcct gtctcgccga ggggtccaag gtcccgcaga gtcgggtcac ccttccctga
     3901 tcccgcctac agccgcggtc tagcgtgagc cacaacagag cggccaagac accgccagca
     3961 acgtggaggg aaaccatgaa tacggcagac agccccgcac ccatcgtcgc agtcaagcga
     4021 cgcaccatcg actc
//
LOCUS       DAORNF010000263         4025 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_16725_length_4025_cov_1.743073, whole genome shotgun sequence.
ACCESSION   DAORNF010000263 DAORNF010000000
VERSION     DAORNF010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4025
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16725_length_4025_cov_1.743073"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..506
                     /locus_tag="PLV68_11025"
     CDS             <1..506
                     /locus_tag="PLV68_11025"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HRE01822.1"
                     /translation="RRGMRVGMVLFVATAAILVMALAMPAFADLFADKRVHGGAWRAA
                     YHVFVRIPLGTVLLEEIALRSVLPAVLALRVGVLKASIISSALFGLWHVLPALNLNEV
                     NPIASDIFGSGLGGQAIAVSFAVASTTLVGLWLCFLRYRAHSVLTTMLAHTASNSIAY
                     ALAWASR"
     gene            565..1386
                     /gene="murI"
                     /locus_tag="PLV68_11030"
     CDS             565..1386
                     /gene="murI"
                     /locus_tag="PLV68_11030"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HRE01823.1"
                     /translation="MFDSGFGGLTVARALIDLLPNEDLVYIADTGRYPYGSRPQSEVR
                     EFARQLAWSLVKDFDAKAIIVACNTAAAAALDTLVDELPVPVISVVEPGAKALVAATR
                     NGRVGVIGTVGTVISGAYDRAVAATGVPVVLTTAACPGFVEFVERGQTSGDEIAILAD
                     RLLAPVKAASVDTLLLGCTHYPYLARVIGQSMGPSVTLVSSADETAFAARTMFANMGL
                     LRPASDGIGGAVTGAHRFLSSGDLAWFADFGGRLFGPELAHAEAWAPPATGQAST"
     gene            1422..1859
                     /gene="rph"
                     /locus_tag="PLV68_11035"
     CDS             1422..1859
                     /gene="rph"
                     /locus_tag="PLV68_11035"
                     /inference="COORDINATES: protein motif:HMM:TIGR01966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HRE01824.1"
                     /translation="MPRPDGRQPNALRPITFERDYTDAAAGSVLVSFGRTKVLCTASV
                     DDDVPRWMRGTGKGWVTAEYSMLPGSSPERVDREAARGKQSGRTVEIQRLIGRSLRAS
                     CDMVALGERQVVVDCDVLQADGGTRTASTSAPTPTPASPCTTR"
     gene            1856..>2036
                     /locus_tag="PLV68_11040"
     CDS             1856..>2036
                     /locus_tag="PLV68_11040"
                     /inference="COORDINATES: protein motif:HMM:NF015670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01825.1"
                     /translation="MTRLVQNGRIDVHPLHSACAAISVGIVGGEAVLDLPYSEDSQAE
                     VDMNVVMLQRLAPLSS"
     assembly_gap    2037..2126
                     /estimated_length=90
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2376..2502
                     /estimated_length=127
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2503..2876
                     /locus_tag="PLV68_11045"
     CDS             <2503..2876
                     /locus_tag="PLV68_11045"
                     /inference="COORDINATES: protein motif:HMM:NF013854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HRE01826.1"
                     /translation="TARFAGNGATDADNRAKMLADLVGIEPPRRTARFRTVAMAMWPD
                     GSELAVEGVCEGSLATAERGARGFGYDPLFIPAAQTAVPPQTFAEMSDEAKNAISHRG
                     QALRALVTALTGDAASEESRE"
     gene            2948..3466
                     /locus_tag="PLV68_11050"
     CDS             2948..3466
                     /locus_tag="PLV68_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HRE01827.1"
                     /translation="MKFKKGDTVIYPQHGACLVQGTKKMEAFGEVQDYLILRTIINEM
                     TLSVPVASAADVGVRPPVSPDELEDLVSVLSKADPRVPSNWSRRFKNHQEKLKSGDVY
                     QVAEVVRNLAARNRDASLSAAERTMYERARINLISEIAPALKVSAEDAEKYLDEALAK
                     GVLKPAKEPKKA"
     gene            3490..>4025
                     /locus_tag="PLV68_11055"
     CDS             3490..>4025
                     /locus_tag="PLV68_11055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01828.1"
                     /translation="MTTRDELTEQIRSGAIDTVIMAFPDTTGRLTGKRTTGRFFLDHV
                     ADHGTENCDYLIACDVDDNPLPGYTFASYDQGYGDMVARPDWSSVRVLPWMQATALVM
                     CDLFRVGQRGADGERATTGAPIAEAPRTILARQVEAAATMGMVPMVASEIEFVLFTQS
                     YAQAHAQGYRRLTPHSPWL"
BASE COUNT          588 a         1306 c         1301 g          613 t
ORIGIN      
        1 ggcggcgggg gatgcgggtc ggcatggtgc tgttcgtcgc gaccgcagcg atcctggtga
       61 tggcgttggc gatgccagcc tttgccgatc tgtttgccga caaacgagtg cacggtggcg
      121 cgtggcgggc ggcctatcac gtgttcgtcc ggattccgct cggcaccgtg ttgttggagg
      181 agatcgcgtt gcgttcggtg ttgccggcag tgctcgcgct gcgtgtcggc gtgctcaagg
      241 cgtcgatcat ctcgtccgca ctgttcgggc tgtggcacgt gctgccggcg ctgaacctca
      301 acgaggtcaa cccgatcgcc agcgacatat tcggcagcgg actcggtggt caggcgatcg
      361 cagtctcgtt cgcggtggcg tcgacgacgc tggtcggact gtggctgtgc tttctgcgct
      421 accgggctca cagcgtgttg acgaccatgc tcgcccacac cgcgtcgaat tcgatcgcct
      481 acgccctggc gtgggcgtcg cgttagtagc cgatgatgtc ggcacgggcc tagtgtgagc
      541 accgctatgc atcgcccgat cggcatgttc gactctggct tcgggggcct gacggtcgcc
      601 cgcgccctca tcgacctgct gcccaacgaa gacctggtct acatcgccga caccggtcgt
      661 tacccgtacg gctcgcggcc ccagagcgag gtgcgcgagt tcgcccggca actggcctgg
      721 agcctggtca aggacttcga tgccaaggcg atcatcgtcg cgtgcaacac ggcggccgcg
      781 gccgcgctcg acacgctggt ggacgagttg ccggtacccg tgatcagcgt ggtcgagccg
      841 ggcgccaagg cgttggtggc cgcgacgcgc aacggtcgcg tcggggtcat cggcacggtc
      901 ggcaccgtga tctccggtgc ctacgaccga gccgtcgccg cgaccggggt gccggtggtg
      961 ctgaccaccg cggcctgtcc tgggttcgtc gagttcgtcg aacggggcca gaccagcggt
     1021 gacgagatcg cgatcctggc cgaccgcctg ctcgctccgg tcaaggccgc ctcggtcgac
     1081 accttgctgt tggggtgcac gcactatccc tatctcgccc gcgtgatcgg ccagtcgatg
     1141 ggtccatcgg tcacgctggt gtccagcgcc gacgagacgg cgttcgcggc tcggacgatg
     1201 ttcgccaaca tggggctgtt gcgccccgca tccgacggca tcggcggggc cgtcacggga
     1261 gcgcaccgct tcttgtcgag cggtgacctg gcctggttcg ccgacttcgg agggcgcctg
     1321 ttcggccccg agttggccca cgccgaagcc tgggcaccgc ctgcaaccgg ccaggcttcg
     1381 acctgaccgc acccgaccac cccccgatct ggagaacacc gatgccccga cctgacggcc
     1441 gtcaacccaa tgccctgcgc ccgatcacgt tcgagcgcga ttacaccgat gccgcggccg
     1501 gctcggtgct ggtcagcttc gggcgcacca aggtgctgtg caccgcgagc gtcgacgacg
     1561 acgtgccccg ctggatgcgc ggcaccggca agggatgggt gaccgccgag tactcgatgc
     1621 tgcccggctc gtcgcccgag cgggtcgacc gcgaagccgc gcgcggcaag cagagcgggc
     1681 gcaccgtcga gatccagcgc ctgatcggcc gttcgctgcg cgcctcgtgc gacatggtgg
     1741 ccctcggtga acgccaggtc gtggtcgact gcgacgtgct ccaggccgac ggcggcaccc
     1801 gtaccgcgtc aacttcggcg cctactccca caccagcatc gccctgcacg acgcgttgac
     1861 gcggctggtg cagaacggcc gtatcgacgt ccacccgctg cattcggcct gtgccgcgat
     1921 cagcgtcggc atcgtcggcg gcgaggcggt gctcgacctg ccctactccg aagatagcca
     1981 ggccgaggtc gacatgaacg tggtgatgct gcaacgcctc gcgccgctga gcagcgnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnntggc cgaaggcggc ctggccgaga tcatggccct
     2161 gcaagccgag atcgtcgtcg ccccacccga accccgccgc ctcgggcgct gacatgagcg
     2221 cagcggagcg gggccgcagc ccccgagcat tgcggcccgt tccggtcttc gtgtgtgcgt
     2281 cggccaaccc gggcaaggtg gccgagatcg ccgcgttgct ggcgggggcg gtcgagttgc
     2341 tgcctcgtcc gctcgatgtg cccgacgtgg tcgaannnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnacaccgcc cgcttcgccg
     2521 ggaacggtgc aaccgacgcc gacaaccgag ccaagatgtt ggccgatctg gtcggcatcg
     2581 aaccgccccg gcgcacggcc cggttccgca ccgtggcgat ggcgatgtgg cccgacggga
     2641 gcgagctcgc cgtcgagggt gtgtgcgaag gatcgctggc caccgccgag cgcggcgctc
     2701 gcggcttcgg ctacgacccg ctgttcatcc cagccgcgca gaccgccgta ccaccgcaga
     2761 ccttcgccga gatgagcgac gaggccaaga acgcgatttc ccaccgcggc caggcgctgc
     2821 gggcgttggt caccgcgctg accggcgacg cggcctcgga ggaaagccgg gaatagcgcc
     2881 gtatcgtggg gattcgtctc ggcgagtcgc gcgttgccga tgaatcactc acagaggtac
     2941 cccaaacgtg aagttcaaga agggtgacac cgtcatctac ccccagcacg gtgcgtgcct
     3001 ggtgcaaggc accaagaaga tggaggcctt cggtgaggtg caggactacc tcatcttgcg
     3061 gaccatcatc aacgagatga cgttgtcggt tcccgtggcc agcgcggccg atgtgggcgt
     3121 gcgtccgccg gtgtcgcccg acgagctcga agacctggtg tcggtgctgt ccaaggccga
     3181 cccgcgcgtg ccgtccaact ggagccggcg gttcaagaac caccaggaga agttgaagag
     3241 cggcgacgtc tatcaggtgg ccgaggtggt ccgtaacctg gctgcccgca accgcgacgc
     3301 gtcgctgtcg gctgccgagc gcaccatgta cgagcgtgct cgcatcaacc tcatcagcga
     3361 gatcgctccc gcgctcaagg tcagcgccga ggatgcggag aagtacctcg acgaagcgtt
     3421 ggccaagggc gtgctcaagc cggccaagga gcccaagaag gcctgaccgg cgggcaggag
     3481 agctggctca tgaccacccg tgacgagctg acggagcaga ttcgctccgg cgcgatcgac
     3541 accgtgatca tggcctttcc cgacaccacg gggcgcctga ccggtaagcg caccaccggg
     3601 cggttcttcc tcgatcacgt tgccgatcac ggcaccgaga actgcgatta cctcatcgcg
     3661 tgcgacgtcg acgacaaccc gttgcccggc tacaccttcg ccagctacga ccagggctac
     3721 ggcgacatgg tggcccgtcc cgactggtcc agcgtgcgcg tgctgccatg gatgcaggcc
     3781 accgcgctgg tgatgtgcga cctgttccgc gtcggccaac gcggggccga cggcgaacgc
     3841 gccacgaccg gcgcgccgat cgccgaagcg ccgcgcacga tcctggcccg tcaggtcgag
     3901 gcggccgcga cgatgggaat ggtgccgatg gtcgcgagcg aaatcgagtt cgtgctgttc
     3961 acccagtcgt acgcccaggc ccacgcccag ggctaccgcc gcctcacccc gcacagcccg
     4021 tggct
//
LOCUS       DAORNF010000264         4017 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_16772_length_4017_cov_1.645381, whole genome shotgun sequence.
ACCESSION   DAORNF010000264 DAORNF010000000
VERSION     DAORNF010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4017
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16772_length_4017_cov_1.645381"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..337)
                     /locus_tag="PLV68_11060"
     CDS             complement(<1..337)
                     /locus_tag="PLV68_11060"
                     /inference="COORDINATES: protein motif:HMM:NF025343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HRE01829.1"
                     /translation="MPEGSGDVEWMLWIDAWGEALRNPLMKTISQELDERSTALLERV
                     LRAGVASGEFRCSDPAASAQRLAGLIDGLAVQFAAHTDLLTREQFIDHVRWLAAAEVG
                     ASIEEFDAAR"
     gene            693..>847
                     /locus_tag="PLV68_11065"
     CDS             693..>847
                     /locus_tag="PLV68_11065"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRE01830.1"
                     /translation="MAERLTALSRSIHGKRAIVTGAASGMGRATAHLFADEGARVAVV
                     DLDPERV"
     assembly_gap    848..863
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <864..>1204
                     /locus_tag="PLV68_11070"
     CDS             <864..>1204
                     /locus_tag="PLV68_11070"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRE01831.1"
                     /translation="DEIRDVHGTEAALPVVCDVSDHAALKRLVALTTETFGGLDILVN
                     NAGIGVPNSAFQDEESYEANWAKILDVNLTAHSRLIRLCLTALQASEAGRVVNIASTE
                     TIVTTPGMAGY"
     assembly_gap    1205..1247
                     /estimated_length=43
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1248..1493
                     /locus_tag="PLV68_11075"
     CDS             <1248..1493
                     /locus_tag="PLV68_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRE01832.1"
                     /translation="VELGRTGITVNCICPGPIRTGMTAQIDEATKEVYAKRRVPLRRY
                     GDPEEVAHMTLNLCLPAASYVNGAIVPVDGGMTIRHV"
     assembly_gap    1571..1581
                     /estimated_length=11
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1749..>2148)
                     /locus_tag="PLV68_11080"
     CDS             complement(1749..>2148)
                     /locus_tag="PLV68_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01833.1"
                     /translation="SFADPRQQPLPGDGSVGLITTFDCIHDMTHPQQMIEAIRAAISD
                     DGTWLLVDIKARDGYSENAQKNPMAALMYGISVLSCMSSAMSAPDGAGLGTLGLSERV
                     AREMATAAGFTRFRKLDVDHSVNAFYEIRP"
     assembly_gap    2149..2273
                     /estimated_length=125
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2274..2882)
                     /locus_tag="PLV68_11085"
     CDS             complement(<2274..2882)
                     /locus_tag="PLV68_11085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01834.1"
                     /translation="MTLSPEASDVPDASPELDPQQLALYSFGLFSTLQGAVTAGMVHL
                     GDRLGLYRAMRDLAHPVTSAELAEAAGLHERWVREWAYNQAAARMISVETPAVDSPGA
                     ERFWLTPEATAVLASPDHPAFGMGMFHRLPQTMHALTGMPECFRTGLGHDYDSHGCEG
                     AIGIERSFEPWNTANLLPVVLPALDGVVERLEAGITAADILIH"
     gene            2938..>3299
                     /locus_tag="PLV68_11090"
     CDS             2938..>3299
                     /locus_tag="PLV68_11090"
                     /inference="COORDINATES: protein motif:HMM:NF022169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2510 domain-containing protein"
                     /protein_id="HRE01835.1"
                     /translation="MTSGADPDWYPDPSGRFEFRYFNGAEWTADVANDGVRFVDPQHQ
                     RPAAPIGTVATHATIDPKAGRGTWGLALLALALAWVPFLFVVGFAAALATLAISVAQS
                     RRAGAGVAHPRQATARVLA"
     assembly_gap    3300..3395
                     /estimated_length=96
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3396..3654
                     /locus_tag="PLV68_11095"
     CDS             <3396..3654
                     /locus_tag="PLV68_11095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01836.1"
                     /translation="QQCAVADGALHAAGTFTNQSARTRSYRLRIDLLRPGTTSRVGGL
                     TVDVDDVAPGATSTWTAQQVTLHAELSCGDVDVTGPMPFGG"
     assembly_gap    3675..3714
                     /estimated_length=40
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3715..3996)
                     /locus_tag="PLV68_11100"
     CDS             complement(<3715..3996)
                     /locus_tag="PLV68_11100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01837.1"
                     /translation="MSDQLNSGDTAEVQQVLATLTGQAELLRRKAPAEVAADTATVAA
                     FTVGLDQLLRDYGYDLDAVADDEQGAVRFGDLTSDDFTAAQRRLVDYAEQ"
BASE COUNT          584 a         1238 c         1257 g          607 t
ORIGIN      
        1 ggcgagcggc atcgaactcc tcgatgctcg cgccgacttc ggccgcggcc agccagcgga
       61 cgtgatcgat gaactgctca cgtgtcagca ggtcggtgtg ggctgcgaat tgcacggcca
      121 gaccgtcgat caacccggcc aggcgctggg cgctcgcggc cgggtcggag cagcggaact
      181 caccggaggc cacccccgcg cgcaacaccc gttccagcaa cgcggtcgat cgctcgtcca
      241 actcttggct gatcgtcttc atcagcgggt tgcgcagcgc ctcgccccag gcgtcgatcc
      301 acagcatcca ctcgacgtcg ccgctgcctt cgggcacgta gttctggatc gcccggtcga
      361 gttggttcgg cctcgatctc tgcgtcgagt tcggccaggt ccttgcgggc gtagtaactg
      421 aatgcctccg cgagcaactg ttccttggaa tcgaagtgat agtggatcaa gctggtcgac
      481 acatcgagtc gcttggccac atccgcgatg cgggtcgaag cgaagccgcg ctcgatcacg
      541 accgcgcagg tcgtctccaa gatctctgcc cgccgctggt cgaccgtctg cttcttcacc
      601 gaggccgaac gctacccacg caggtcgctc cgctgcgacg attcaggctg cggcaatccc
      661 tcgacaccga gtcggcgtct atggtcgatt ccatggctga acgactcacg gcactgtccc
      721 gttccatcca cggcaagcga gcgatcgtca ccggcgcggc gtcgggcatg ggtcgggcga
      781 cggcccacct gttcgccgac gagggagcgc gcgtcgcggt ggtcgatctc gaccctgagc
      841 gagtgcannn nnnnnnnnnn nnncgacgag atccgcgacg tccacggcac cgaggcggca
      901 ttgccggtcg tgtgcgacgt gagcgaccac gccgcgctca agcgtctcgt cgcgctcacc
      961 acagagacgt tcggtgggct cgacatcttg gtcaacaacg ccggtatcgg ggtgccgaac
     1021 tcagcgtttc aggacgaaga gagctacgag gccaactggg ccaagatcct cgatgtcaac
     1081 ctcactgccc attcccgtct gatccggctg tgcttgactg ccctgcaggc gtcggaggca
     1141 gggcgggtcg tcaacatcgc ctcgaccgag acgatcgtga ccaccccagg aatggcgggg
     1201 tacgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngtc gagctgggcc
     1261 gcacgggcat cacggtcaac tgcatctgcc ctggtccgat ccgcaccggg atgaccgcgc
     1321 agatcgacga ggcgaccaag gaggtgtacg ccaagcgacg ggtgccgctg cgccgctacg
     1381 gcgaccccga agaggttgct cacatgaccc tcaacctgtg cctaccagcg gccagctatg
     1441 tcaacggcgc gatcgtcccc gtcgacggcg gcatgaccat ccgccacgtg tagcgcacgg
     1501 cgcgcaccgt gtcgggttga ccccgccagg aggggtgaac cgaccacact cgctgcgcgg
     1561 ggcgggcacc nnnnnnnnnn natgaggggt ggagtggaca cactcgccgc gggcggcccg
     1621 gactgtgtcg ggttggcccc tccagcgggg gtggagcgaa cacacttgcc gtcgctcgcc
     1681 acgactgtgt cgggttggcc ctgctggcgg gggcggatcg aacattgttg tggcgtgcaa
     1741 cagcggggtt atgggcggat ttcgtagaag gcgttgacgg agtggtcgac gtcgagcttg
     1801 cggaagcgag tgaagccggc agccgtggcc atttcgcgag cgacccgttc gctgaggccg
     1861 agcgtgccca agcccgcgcc gtcgggggcc gacatcgccg acgacatgca cgacagcacg
     1921 ctgatgccgt acatcaacgc agccatcggg ttcttctggg cgttctcgct gtagccgtct
     1981 cgcgccttga tgtcgacgag caaccatgtg ccgtcgtcgc tgatcgcggc ccggatcgcc
     2041 tcgatcatct gctgcgggtg ggtcatgtcg tggatgcagt cgaacgtggt gatcagcccg
     2101 accgacccat cgcccggcag cggctgctgc cgcggatcgg cgaacgacnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngtggatg
     2281 aggatatccg ccgcggtgat ccccgcctcg agtcgctcga ccaccccatc gagcgcgggc
     2341 agcaccactg gtaacaggtt ggccgtgttc cacggctcga agctgcgctc gatgccgatc
     2401 gcgccctcgc agccgtggct gtcgtagtcg tggccgaggc cggtgcggaa gcactcgggc
     2461 atgccggtga gcgcgtgcat cgtctgtggc aggcggtgga acattcccat cccgaacgcc
     2521 ggatggtcgg ggctggcgag caccgcggtg gcctcggggg tcagccagaa ccgctccgcg
     2581 ccaggcgagt cgacggcggg cgtctcgacg ctgatcatcc gcgccgcggc ctggttgtag
     2641 gcccactcgc gcacccagcg ctcgtgcagt ccggcggcct cggccaactc ggccgaggtg
     2701 acgggatgcg cgaggtcgcg catcgcgcga tagaggccga gacggtcacc gaggtgcacc
     2761 atgccagcgg tgactgcccc ctgcagggtg ctgaagagac cgaacgagta cagcgccagt
     2821 tgctgcggat cgagctcggg cgatgcatcc ggcacgtctg atgcttctgg tgaaagggtc
     2881 atcggcgcag ggtagtggcc gacttcgcag tggctcgacc ggtagcgtgc tccaggcatg
     2941 acgagcggcg cggacccgga ctggtacccc gatcccagcg gcagattcga gtttcgctac
     3001 ttcaacggcg ccgaatggac cgctgacgtc gccaacgacg gtgtccgctt cgtcgatccg
     3061 cagcatcagc gccctgcagc cccgatcggt acggtcgcga cccacgccac gatcgacccg
     3121 aaggccggcc gtggcacatg gggactggcg ttgctcgcgc tggccttggc ctgggtgccg
     3181 ttcctgttcg tggtcggctt cgccgcagca ctcgcgacct tggcgatctc ggtcgcgcaa
     3241 tcgaggcgcg ccggtgccgg cgtcgcccat ccgcgccagg cgacggcgcg cgtgctcgcn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnccagc agtgcgcggt cgccgacggc
     3421 gcactgcacg ccgcgggcac gttcaccaac cagtccgcca ggacacgctc gtatcggctt
     3481 cgcatcgatc tgttacggcc cggcacgacc agccgcgtcg gcggcctgac cgtcgacgtc
     3541 gacgacgtgg caccgggagc gacgtcgacg tggacggcgc aacaggtcac gctacacgcc
     3601 gaactcagct gcggcgacgt cgacgtcacc ggtccgatgc catttggcgg atagcggtcc
     3661 ggcggatggc cgagnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnttgctc
     3721 ggcgtagtcg accagacgcc gctgcgcggc ggtgaagtcg tcactggtca gatcaccgaa
     3781 gcggaccgca ccctgctcgt cgtcggccac tgcgtcgagg tcgtagccgt agtcgcgcaa
     3841 cagttgatcg agcccgacgg tgaacgcggc aaccgtcgcc gtgtcggcgg caacttcggc
     3901 cggcgccttg cggcggagta actcggcctg gccggtcagg gtggccagca cctgctgcac
     3961 ctcggctgtg tcgccgctgt tgagctgatc gctcaaccca ccgttggtgg tttggaa
//
LOCUS       DAORNF010000265         4014 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_16789_length_4014_cov_1.552665, whole genome shotgun sequence.
ACCESSION   DAORNF010000265 DAORNF010000000
VERSION     DAORNF010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4014
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16789_length_4014_cov_1.552665"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>313
                     /locus_tag="PLV68_11105"
     CDS             <1..>313
                     /locus_tag="PLV68_11105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01838.1"
                     /translation="ISAVITEAAGTATTPADYTLTTTSISFPAGSLNAATAPVSSTIV
                     NDALIEGPHTFTLGFGAVTTDMTGVSASGTHVVTITDDDFAGVTIVQTGGSANVTAGS
                     TT"
     assembly_gap    314..506
                     /estimated_length=193
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <507..2442
                     /locus_tag="PLV68_11110"
     CDS             <507..2442
                     /locus_tag="PLV68_11110"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HRE01839.1"
                     /translation="ADFAAITPNSISVTVNITDNDVPGVVITESGTTDVIEGGATDTY
                     DVVLLGAPTQDVTITLTSVGAQLTLPAPLTFTPANYNVPQTITVQAVDDGIYEGSHTD
                     TITHTLTSLDLNYNGFPAGSVTVFIGDGISELILNGSFETPGATAKEAQNWKGSSLTS
                     LDRRICNPSKAFEGSCAFQFNFSGPLVVGRKLKQVTAAPAWGFPGETLTLNAQVSTNG
                     FTTGAKMRIKVIYDDATVEKVQVAIPAGTYPYSPITTSLTLSKRVTKAIVMFKVGSAV
                     GRVWIDDVSFTLRSADFSRDIEANGTGDSVRRRHREERTARAVEANQEVAKSILNLDP
                     PDHTRLRRLVTLAFTPSAIERLRPRVRELVDAALERAAERGEMELIDDLAFPMPFQVI
                     SDLLGMPTERADELREWSQVLTQSLEPTTTEAELRLADHAASQFVAYIVDVIEHRRRH
                     LGDDLLSALIQAEEAGDRMSNAELISFTLLLYVAGHETTVNLIGNGTLALLRNPGELV
                     RWRDDPSLDAVAVDELLRYDGPVQLTVRVPMKPVSYGEVTVEPGETVMTVLGAANHDP
                     AVFDDPHTLRLGRANANRHLAFAAGIHYCLGSSLAKLEATEAISRLIRRFPDVSLGGE
                     PGWRDRLTIRGVDRLPLTLG"
     gene            complement(2461..3339)
                     /locus_tag="PLV68_11115"
     CDS             complement(2461..3339)
                     /locus_tag="PLV68_11115"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRE01840.1"
                     /translation="MGQVAFIGLGVMGAPMARHLNNKGHDVTVYNRTASKAEAWISAH
                     GGWMAGTPAEAAADADVVFLCVGNDDDVRSVVYGDEGVLAAMRAGSILVDHTTASADL
                     ARELGAAAAAAGVGFVDAPVSGGQAGAERGALTVMCGCDDVLTFRKAQSVIAAYSQSC
                     ELMGDVGAGQLTKMVNQICIAGLAQALAEGVNFAQHAGLDVTKVVQVIGKGAAQSWQM
                     DNRAITMAQDKYDFGFAVEWMRKDLGICFAEAERNGAELPVTRLVDSYYAEVIEAGGR
                     RWDTSSLMYRLTHPKG"
     gene            complement(3404..>4014)
                     /locus_tag="PLV68_11120"
     CDS             complement(3404..>4014)
                     /locus_tag="PLV68_11120"
                     /inference="COORDINATES: protein motif:HMM:NF012348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01841.1"
                     /translation="TWPRPARRAMSIPACGASTAPAPCSPPTRRRTAIGRARCSATTS
                     PGCSPRPASSRCCGLQRSTATSAISTAPTGISWGYDNRTLGFRIVGHGASTRVEGRIP
                     GADANSYHAFAGTIAGGLYGIRHRLELGPAFVGNGYEATDIPRVPTTLVEAIELWEHS
                     AIARECFGDDVHHHILTTAKGEWAAFNQSVTDWELRRYWERI"
BASE COUNT          661 a         1353 c         1201 g          606 t
ORIGIN      
        1 catctcggca gtcatcaccg aggcggccgg cacggccacc acgccggcgg actacacgct
       61 gacgacaacc tcgatcagct tcccggccgg cagcctgaac gccgcgacgg cgccggtctc
      121 ctccaccatc gttaacgacg cgctgatcga aggcccgcac accttcacgc tcggcttcgg
      181 cgcggtcacg accgacatga ccggcgtcag cgccagcggc acgcacgtgg tcaccatcac
      241 cgatgacgac ttcgccggcg tgacaatcgt tcagaccggc ggatcggcta acgtgaccgc
      301 aggcagcacg accnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      481 nnnnnnnnnn nnnnnnnnnn nnnnnncgcg gacttcgcgg cgatcacgcc caacagcatc
      541 tcggtgacgg tcaacatcac cgataacgac gtgccgggtg tagtcatcac tgaaagcggc
      601 acgacggatg tcatcgaagg cggcgcgacc gacacttacg atgtcgtgct gctcggcgcc
      661 ccgacgcagg acgtcacgat caccctgacc agcgtgggcg cacagctcac actgccggct
      721 ccgctgacct tcacgccagc caactacaac gtgccgcaga ccatcacggt gcaggcggtg
      781 gacgacggta tctatgaagg ttcgcacacc gacacgatca cccatacgct aaccagcctg
      841 gacctgaact acaacggctt cccggcaggc agcgtaacgg tattcatcgg tgacggcatc
      901 agcgagctga tcctgaacgg cagcttcgag acgccgggcg cgaccgccaa ggaagcgcaa
      961 aactggaagg gttccagcct gaccagcctg gatcgccgca tctgcaatcc gagcaaagcc
     1021 tttgaaggat cgtgcgcgtt ccagttcaac ttctccggcc cgctggtcgt cggccgcaag
     1081 ctcaaacagg tcacggcggc gccagcctgg ggcttccccg gcgagacgct gacgctcaat
     1141 gcgcaggttt ccactaacgg cttcaccacc ggcgcgaaga tgcggatcaa ggtcatctac
     1201 gacgacgcaa cggtcgagaa ggttcaggtc gccatcccgg ctggcaccta tccttactcg
     1261 ccgatcacca cctcgctcac tttgtcgaaa cgggtgacca aagccatcgt gatgttcaag
     1321 gtgggcagcg cggtcggccg ggtctggatc gacgacgtgt cgttcacgct gcgcagcgcc
     1381 gacttcagcc gcgacatcga ggccaacggc acgggcgact cggtgcgccg ccgccatcgt
     1441 gaagaacgca cggcgcgcgc ggtcgaggcc aaccaggagg tcgccaagtc gatcctcaac
     1501 ctcgacccgc ccgaccacac ccgtctgcgc cgtctggtga ccctggcgtt cacaccctcg
     1561 gcgatcgagc gcctgcggcc acgggtacgc gagctggtcg atgccgcact cgagcgtgcc
     1621 gccgagcgag gcgagatgga gttgatcgac gacctcgcct ttccgatgcc gttccaggtg
     1681 atctccgatc tcctcgggat gcccaccgag cgggccgacg agttgcgcga gtggtcgcag
     1741 gtgctcaccc agtcgttgga gccgaccacc accgaagccg aactccggtt ggccgaccac
     1801 gccgcgagcc agttcgtcgc ctacatcgtc gacgtcatcg aacaccgccg ccgtcacctc
     1861 ggcgacgatc tgctgtcggc gctgatccag gccgaagagg cgggtgaccg gatgagcaac
     1921 gccgagctca tctccttcac cctgctgctc tacgtagccg gtcatgagac cacggtcaac
     1981 ctgatcggca acggcacgtt ggcgctgctg cgcaaccctg gcgagttggt ccgctggcgc
     2041 gacgacccca gcctcgatgc ggtagcggtg gacgagttgc tgcgctacga cggcccggtg
     2101 caactcacgg tgcgggtgcc gatgaagccg gtcagctacg gcgaggtaac ggtcgagccc
     2161 ggcgagacgg tgatgacggt gctcggcgca gccaaccatg acccggccgt tttcgacgac
     2221 ccgcacacgc tgcgcctcgg gcgggccaac gccaaccgcc atctggcctt cgcggcgggc
     2281 atccactatt gcctcggctc gtcgctggcc aagttggaag ccaccgaggc gatcagccgc
     2341 ctcatccgtc gcttccccga cgtcagcctg ggcggcgaac ccggctggcg cgaccgcctc
     2401 accatccgcg gcgtcgaccg cctccccctc accctcggtt gaggcgcgtc gcgtcgcgcg
     2461 tcaacccttg gggtgggtga gacggtacat caggctgctg gtgtcccacc ggcggccgcc
     2521 ggcttcgatc acttcggcgt agtagctgtc gaccaagcgg gtcacgggca gctcggcgcc
     2581 gttgcgttcg gcttcggcaa agcagatccc gaggtccttg cgcatccact cgaccgcgaa
     2641 gccgaagtcg tacttgtcct gcgccatcgt gatcgcccgg ttgtccatct gccagctctg
     2701 cgccgcgccc ttgccgatca cctgcaccac cttggtcacg tcgagtccgg cgtgctgagc
     2761 aaagttgacg ccctcggcca gcgcctgagc caacccggcg atgcagatct ggttgaccat
     2821 cttcgtcaac tgtccggcgc cgacgtcgcc catcagctcg catgactgcg agtaggcggc
     2881 aatcaccgat tgggccttac ggaacgtgag cacgtcgtcg cagccgcaca tcaccgtcaa
     2941 cgcgcccctc tcggcaccgg cctggccacc gctgaccggc gcgtcgacaa accccacgcc
     3001 cgcagccgca gcggccgcgc ccaactcacg cgccagatcg gccgacgccg tggtgtgatc
     3061 gaccaggatc gacccggcgc gcattgcggc cagcacgccc tcgtcgccgt agaccaccga
     3121 gcgcacgtcg tcgtcgttgc cgacgcacag gaacacgacg tcggcatcgg ctgctgcttc
     3181 ggccggagtg ccggccatcc atccgccgtg ggcagagatc cacgcttcgg ccttgctcgc
     3241 cgtgcggttg tagacggtga cgtcgtggcc cttgttgttg agatggcgcg ccatcggcgc
     3301 acccatcact ccgagtccga tgaaggcgac ctgtcccatg agccagattc tcgcaggtcg
     3361 cacgcaagtt tgcgcacgcc gtgtgcacac ccgcgcctgg acgtcagatc cgctcccaat
     3421 aacggcgcaa ctcccagtcg gtgaccgact ggttgaacgc cgcccactcc cccttggccg
     3481 tcgtgaggat gtggtggtgc acgtcgtcgc cgaagcactc gcgggcgatg gcgctgtgct
     3541 cccacaactc gatcgcctcg accagcgtgg tcggcacccg cgggatgtcg gtggcttcgt
     3601 agccgttgcc gacgaacgcg gggccgagct cgagtcggtg gcggatgccg tacaggccac
     3661 cagcgatggt gcccgcgaac gcgtggtagc tgttcgcgtc ggcgccggga atgcgcccct
     3721 cgacacgggt ggaggcgccg tggccgacaa ttcgaaaccc gagggtgcgg ttgtcgtagc
     3781 cccaactgat gccggtgggc gccgtgctga tagcgcttgt agctgttgac cgttggagcc
     3841 cacagcagcg tgaactcgcg ggccgtggcg agcaacccgg cgaggtagtg gccgaacaac
     3901 gcgctcggcc gatcgccgtg cgtcgcctcg tcgggggcga acaaggcgcg ggtgccgtcg
     3961 aggctccaca ggctggaatg gacatggcac gacgagccgg tctcggccat gtcc
//
LOCUS       DAORNF010000266         4004 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_16838_length_4004_cov_1.867055, whole genome shotgun sequence.
ACCESSION   DAORNF010000266 DAORNF010000000
VERSION     DAORNF010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4004
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16838_length_4004_cov_1.867055"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..739)
                     /locus_tag="PLV68_11125"
     CDS             complement(<1..739)
                     /locus_tag="PLV68_11125"
                     /inference="COORDINATES: protein motif:HMM:NF015297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HRE01842.1"
                     /translation="MATPQLIRTLVFLGGHDLARIEQVAAAGPDAICIDLEDSTPLAK
                     KDAARTLFGEAVAAITAAGALVVVRTNMPGAGEDEDLAAVMCPQLHAVSLPKVESAEA
                     VQTYDETVARAERAAGLEPGAVLIRPIIETAQGVRMAYEISAASPRVAYLGGVEGGIF
                     GDLGGSLGYVQTDNGMETFFLRSKVLIDARAAGVPFPIGGGMTTRRDADGARSFAREN
                     RILGYNGVHCAADPEVIKAVNDEFTPDR"
     assembly_gap    862..935
                     /estimated_length=74
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<936..>1702)
                     /locus_tag="PLV68_11130"
     CDS             complement(<936..>1702)
                     /locus_tag="PLV68_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lipid-transfer protein"
                     /protein_id="HRE01843.1"
                     /translation="ARAQILAGFCDIALVIGADTTPKGFFAPVGGERRDDPDWQRFHV
                     LGATNPSYFALLARRRIDLYGATPDDFAQVKVKNARHGATNPNARFTKQYDVAEVLAS
                     PVVSDPLRLFDICATSDGAAAMVVCSMDYARRHLGSADGICRVAAVGTVTPVYPQTFL
                     ELPDIATDSSAVVAPPARKYKDSIAWSAYEQAGLGPADIDVAEVYDLSTALELDWYEH
                     IGLCAEGEAEGLLRSGATTIGGRVPVNPSGGLSCFGE"
     assembly_gap    1703..1728
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1729..1996)
                     /locus_tag="PLV68_11135"
     CDS             complement(<1729..1996)
                     /locus_tag="PLV68_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-transfer protein"
                     /protein_id="HRE01844.1"
                     /translation="MSTDRDIAILGVGMHPWGKWGRDFTEYGVVAARAALADAGVAWT
                     DIQYVAGADTIRNGYPGFIAGSTFAQKLGWTGARISSNYAACASG"
     gene            complement(1993..2460)
                     /locus_tag="PLV68_11140"
     CDS             complement(1993..2460)
                     /locus_tag="PLV68_11140"
                     /inference="COORDINATES: protein motif:HMM:NF013920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HRE01845.1"
                     /translation="MTGWFTERSATGTNRPALTGQRCGTCGTYVFPPRSGGCPSPHCT
                     ADELESVELSSAGRVWSYTENRYAPPPPYVAPDPFVPYALAAVELEREGIVVLGKVGD
                     GLLAADLRVGMPMRLGLEVSHRDGDHDVIVYTWVPDTGINADAGTNADDGSQP"
     gene            2554..>2918
                     /locus_tag="PLV68_11145"
     CDS             2554..>2918
                     /locus_tag="PLV68_11145"
                     /inference="COORDINATES: protein motif:HMM:NF014790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRE01846.1"
                     /translation="MDLNESAEERQFRAEARRWLQANVPAEPLPSFDTARGFALHREW
                     EHRLAADRWSAVSWPVEYGGRGADYLKWLIFEEEYWRSGAPGRVSQNGIFLLGPTIME
                     VGTEEQKARGLYAPGEGIIG"
     assembly_gap    2919..2928
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2929..>3313
                     /locus_tag="PLV68_11150"
     CDS             <2929..>3313
                     /locus_tag="PLV68_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRE01847.1"
                     /translation="EEIWPQAWSEPDAGSDMAAIRSRGRLSDDGTEWILDGQKIWASR
                     AVWADWCFGIFRTDPDSARHRGLTFILIPLDTPGVTVRPIAQLDGDTGFAEIFFDGAR
                     VPVENTLGPVGGGWGVAMATAGFERG"
     assembly_gap    3314..3340
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3341..3722
                     /locus_tag="PLV68_11155"
     CDS             <3341..3722
                     /locus_tag="PLV68_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRE01848.1"
                     /translation="VAVDRLITLWAASADRDDDSLRDAVTDVWMRAEGYRLHTLMTVT
                     KMLDGQPIGSESSLNKIFWSEMDVRTHELALQLLGPEAEHMDGPNESWVDGFLFSLSG
                     PIYAGTNEIQRNIIADRVLQLPRA"
     gene            3736..>4004
                     /locus_tag="PLV68_11160"
     CDS             3736..>4004
                     /locus_tag="PLV68_11160"
                     /inference="COORDINATES: protein motif:HMM:NF014790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRE01849.1"
                     /translation="MQFRFDSEQIEFAAGLRELLQKEFTASHLRAVWDAGRGHDDALW
                     SKLAGLGVFGMLVPEDNGGFGGDMVDAVLLAEEFGRAAVPGPAFET"
BASE COUNT          605 a         1287 c         1356 g          619 t
ORIGIN      
        1 ggcggtcagg agtgaactcg tcgttgaccg ccttgatcac ctcggggtcg gcagcgcagt
       61 gcaccccgtt gtagccgagg atgcggttct cgcgggcgaa gctgcgcgcg ccgtcggcat
      121 cgcggcgtgt cgtcatgccg ccaccgatcg ggaacggcac gccagcggca cgcgcgtcga
      181 tcagcacctt gctgcgcagg aagaacgttt ccatcccgtt gtcggtctgg acgtagccca
      241 gcgatccgcc gaggtcgccg aagatgccac cttcgacccc gccgaggtag gcgacccgcg
      301 gcgaggcagc ggagatctca tacgccatcc gcacaccctg ggcggtctcg atgatcgggc
      361 ggatcaacac cgcacccggc tccaggcctg ccgcgcgctc ggcacgggcg accgtctcgt
      421 cgtaggtctg gacggcttcg gccgactcga ccttgggcag gctcaccgcg tgcagctgcg
      481 ggcacatgac agcggccagg tcttcgtcct ctccggcgcc gggcatgttg gtgcgcacca
      541 ccaccagagc acctgccgcg gtgatcgcgg caaccgcttc cccgaacaac gtcctggccg
      601 cgtccttctt ggccaggggt gtcgagtctt cgagatcgat gcagatcgca tcgggtcccg
      661 cggcggcgac ctgctcgatc cgcgccagat cgtgaccgcc caggaagacc agcgtgcgga
      721 tcagttgtgg tgttgccatg acgcccccgt gtcgtgttcg tgtgtcgtgt tcgtgtgtcg
      781 cgtcggtgtg tttcagcgat ggacgatgac gctgctgccg tgaccgaaca ggccctggtt
      841 ggcggtgatg ccgacccgtg cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnttcac cgaagcagct caacccgccg
      961 ctggggttga ccggcacccg cccgccgatg gtcgtcgcac ccgagcgcag cagaccctcg
     1021 gcctcgcctt cggcacacaa gccgatgtgc tcgtaccaat cgagttcgag cgccgtcgac
     1081 aggtcgtaca cttcggccac gtcgatgtcg gcgggaccga gaccggcctg ttcgtaggcc
     1141 gaccaggcga tcgagtcctt gtacttgcgc gccggcggcg cgaccacggc cgacgagtcg
     1201 gtggcaatgt cgggcagttc gaggaacgtc tgcgggtaca ccggcgtcac cgtgcccacg
     1261 gccgcgaccc ggcagatgcc gtcggccgac ccgaggtggc gacgggcgta gtccatactg
     1321 cagacaacca tcgccgcggc gccgtcgctg gtggcacaga tgtcgaacag ccgcagcggg
     1381 tcggacacga ccgggctggc gagcacctcg gcgacgtcat actgcttggt gaagcgggcg
     1441 ttggggttgg tggcaccgtg acgggcgttc ttcaccttga cttgggcgaa gtcgtcgggc
     1501 gtcgccccgt acaggtcaat gcggcggcgc gccagcagcg cgaagtacga cgggttggtg
     1561 gcaccgagca cgtggaaccg ctgccagtcc ggatcgtcgc ggcgctcccc accgacgggg
     1621 gcgaagaagc ccttgggggt ggtgtcggcc ccgatcacca gggcgatgtc gcagaacccg
     1681 gccaggattt gggcccgcgc ggnnnnnnnn nnnnnnnnnn nnnnnnnncc cccgaggcgc
     1741 acgcggcgta gttgctggag atgcgcgccc ctgtccagcc gagcttctgg gcaaacgtcg
     1801 agccggcgat gaaacctggg tagccattgc gaatcgtgtc ggcaccggcg acgtattgga
     1861 tgtcggtcca ggcgacgccg gcgtcggcta gtgcggcgcg ggccgccacc acgccgtatt
     1921 cggtgaaatc acggccccac ttgccccacg ggtgcatgcc taccccgagg atggcgatgt
     1981 cgcggtcggt gctcacggct ggctcccatc atcggcgttg gtgccggcat cggcgttgat
     2041 gccggtgtcg ggcacccacg tgtagacgat cacgtcatga tcgccgtccc gatgcgacac
     2101 ctccaggccg agacgcatcg gcatgccgac gcgcaggtcc gccgcgagca gcccgtcacc
     2161 gaccttgccc agcaccacga tgccctctcg ttccagctcg accgcggcga gcgcgtacgg
     2221 cacgaacggg tcgggggcga cgtacggcgg cggtggcgcg tagcggttct ccgtgtagct
     2281 ccacacccga ccggccgaac tgagttcgac cgattcgagt tcgtcggcgg tgcagtgcgg
     2341 tgacggacaa ccacccgagc gaggaggaaa gacgtacgtg ccgcaggtgc cacatcgctg
     2401 gccggtcagc gccgggcggt tggtgccggt cgcggatcgc tccgtgaacc atccggtcat
     2461 gatcgggcgc gtcgcggccg tgtcggcgag gtccgcgccc ggttccgagt tcgacagtga
     2521 agtactaatg atttaaaagt tagcatccag agcatggatc tgaacgaatc agccgaggag
     2581 cgccagttcc gtgccgaagc gcgccgctgg ttgcaggcca acgtgcccgc cgagccgttg
     2641 cccagcttcg acacagcacg cggcttcgcc ctgcaccgcg agtgggaaca ccgccttgcc
     2701 gcggaccggt ggtcggcggt gagctggccg gtcgagtacg gcggccgcgg cgccgactac
     2761 ctcaagtggc tgatcttcga ggaggagtac tggcgcagcg gcgcccccgg gcgggtcagc
     2821 cagaacggca tctttctcct cggccccacg atcatggagg tcgggaccga ggaacagaag
     2881 gcgcgcggcc tctatgcgcc gggcgagggt atcatcggnn nnnnnnnngt gaggagatct
     2941 ggcctcaggc ctggagcgag cccgacgccg gcagcgacat ggcggcgatc cgtagtcggg
     3001 gccggttgag cgacgacggt accgagtgga tcctcgacgg ccagaagatc tgggccagcc
     3061 gggcggtgtg ggccgactgg tgcttcggca tcttccgcac cgacccggac agcgcgcgcc
     3121 accgcggtct caccttcatc ctcatcccac tcgacacgcc gggggtgacg gtgcggccga
     3181 tcgctcagct cgacggtgac accggcttcg cggagatctt cttcgacggt gcccgggtgc
     3241 cggtcgagaa cacgctcggc cccgtcggcg gcggctgggg cgtcgccatg gcgacggccg
     3301 gcttcgaacg tggnnnnnnn nnnnnnnnnn nnnnnnnnnn cgtcgccgtc gatcggctga
     3361 tcacgctgtg ggccgcgtcg gcagatcgtg acgacgactc cttgcgtgat gcggtgaccg
     3421 acgtctggat gcgggccgag gggtatcggc tgcacacgtt gatgaccgtc accaagatgc
     3481 tcgacggcca accgatcggc agcgagtcga gcctcaacaa gatcttctgg agcgagatgg
     3541 acgtgcgcac ccacgagctc gccctgcagc tgctcggccc cgaggcagag cacatggacg
     3601 ggccgaacga gtcgtgggtc gacggcttcc tgttcagcct gagcggaccg atctacgccg
     3661 ggaccaacga gatccagcgc aacatcatcg ccgaccgcgt gctgcaactg ccgcgggcct
     3721 gaagggacac caaccatgca gttccgattc gactccgaac agatcgagtt cgccgcgggc
     3781 ttgcgcgaac tgttgcagaa ggagttcacc gcgtctcacc tgcgcgccgt ctgggatgcc
     3841 ggccgcggcc acgacgacgc gctgtggtcc aagttggccg gcctcggcgt gttcggcatg
     3901 ctcgtgccgg aggacaacgg tggcttcggc ggcgacatgg tcgacgccgt gctgttggcc
     3961 gaagagttcg gccgcgccgc ggtgccgggg ccggcgttcg agac
//
LOCUS       DAORNF010000267         4001 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_16856_length_4001_cov_1.459453, whole genome shotgun sequence.
ACCESSION   DAORNF010000267 DAORNF010000000
VERSION     DAORNF010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 4001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4001
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16856_length_4001_cov_1.459453"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="PLV68_11165"
     CDS             complement(<1..301)
                     /locus_tag="PLV68_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HRE01850.1"
                     /translation="MSTADTTTLPRRPGMTVPLPGPLHSHQAKLTELADLGYTDVWSA
                     ESNAADAFTPLALAAAWEPRLRLGTAIVPAYTRSPGVFAQSVASLADAAPGRFAIG"
     gene            complement(319..>539)
                     /locus_tag="PLV68_11170"
     CDS             complement(319..>539)
                     /locus_tag="PLV68_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HRE01851.1"
                     /translation="LDNTIRIARLVPTEWVLLDIQVHAVSRGFGHGLVQMFAEDGTLM
                     ATASQSCIVRYWKDDRPPKPRFDSVESA"
     assembly_gap    540..676
                     /estimated_length=137
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<677..>979)
                     /locus_tag="PLV68_11175"
     CDS             complement(<677..>979)
                     /locus_tag="PLV68_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HRE01852.1"
                     /translation="AKPGDTMDIDVVIAVEGHQITQARALCHVGNREILTVNAALGHR
                     DIDLGGQWEQMPPAAPPLECEERSHLLPPAGYLHDHVEQRWVTGRAYADLGGTHGS"
     assembly_gap    980..1095
                     /estimated_length=116
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1381..1413
                     /estimated_length=33
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1414..2636
                     /locus_tag="PLV68_11180"
     CDS             <1414..2636
                     /locus_tag="PLV68_11180"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRE01853.1"
                     /translation="GLSLYGVTYAVYMLADLVALQWSSETADRYGVRRPFLVSVALFV
                     IGLLIAASAPTMELVVLGRGLQGAGSGGFAPLTFITVQRAFPESRRASLFALLSAGWV
                     LPTLIAPAISGLVTDHFGWRLVFLGILPIALAVATVTSRAMVAVPPPDLAVERRASLL
                     PNSARTAIGIAMVAFGAQFAQWWLAAGLCISGSAIAAKPLRSLLPKGFFRARSGLAAI
                     MVVRFLSTAMFLGVDSFVPLAADRLHGARPIVQGFTIAGAAVLWSLGQAFAARRGTRL
                     LPSRGAAIGFALMLIGAGATVPVIAPSWPLWATFISWSVAGFGMGIIFNPTTVSAMTY
                     ADDSREGEIGGQLHLADSLGFGCMSILGGATVAIADRTSFSLQGALGTNFSMALICGV
                     IGLIVAHRVHARTT"
     assembly_gap    2863..3042
                     /estimated_length=180
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3043..>3281
                     /locus_tag="PLV68_11185"
     CDS             <3043..>3281
                     /locus_tag="PLV68_11185"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRE01854.1"
                     /translation="LSHRSGIGDYLDEEAYEITDYVMRRPVHELDTTEAFLPLLDGFP
                     MVAVPGEQFAYNNGAFVVLALLIERATGQSYHDVV"
     assembly_gap    3282..3531
                     /estimated_length=250
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3532..3759
                     /locus_tag="PLV68_11190"
     CDS             <3532..3759
                     /locus_tag="PLV68_11190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01855.1"
                     /translation="TRPRSHSAEEEARYGLGFWLGEYDDSIELRGYDAGVSFHAVHQP
                     STGTTYSIIANWTDGAWPLARLLTATFAPAA"
     gene            complement(3793..>4001)
                     /locus_tag="PLV68_11195"
     CDS             complement(3793..>4001)
                     /locus_tag="PLV68_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01856.1"
                     /translation="GSVDDGWGGMLGWFWVACAVTTWALCWVTQRALTEKYYSAVMAA
                     TGLLYLAILTAFGTDLVARAILAG"
BASE COUNT          479 a         1154 c         1109 g          543 t
ORIGIN      
        1 tgccgatcgc gaaccggccc ggcgccgcat cggccagcga ggccacgctc tgtgcgaaca
       61 cgcccggcga tcgggtgtag gcgggcacga tcgccgtgcc gaggcgcaac cgcggctccc
      121 aggcagcggc caaggccaac ggggtgaacg cgtcggcggc gttcgactcg gccgaccaga
      181 cgtcggtgta gcccaggtcg gccagctcgg tcagcttggc ctggtggctg tgcagcggcc
      241 ctggcagcgg cacggtcatg ccgggacggc ggggcaaggt ggtcgtgtcg gctgtgctca
      301 tgggcacagc ttgcccgatc aggccgactc caccgaatcg aaccgcggct tgggcggccg
      361 gtcgtccttc cagtagcgca cgatgcaact ctggctggcc gtcgccatca acgtgccgtc
      421 ctcggcaaac atctgcacca acccgtgtcc gaagccccgg ctgaccgcgt ggacctggat
      481 atcgagcagc acccactcgg tcggcaccag gcgggcgatg cggatggtgt tgtcgaggcn
      541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      661 nnnnnnnnnn nnnnnnccga accgtgcgtg ccgccgaggt cggcgtaggc acgacctgtc
      721 acccaacgct gctcgacgtg atcgtgcagg tatccagccg gaggcagcag gtggctgcgc
      781 tcttcgcact ccagcggcgg cgcggccggt ggcatctgct cccattgacc gcccaggtcg
      841 atgtcacggt gcccgagcgc ggcattgacg gtgaggatct cacggttgcc gacgtgacac
      901 agcgcacggg cttgggtgat ctggtggccc tcgacggcga tgaccacgtc gatgtccatc
      961 gtgtcacccg gtttggcgtn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnncgtcg acacccgctt ggtgacgggc agaatccagc gatagggatt
     1141 gtgggtgggt tcgagcccga ggaaggcgac actgtccatg gccctgcgga ctctacgtga
     1201 cgttccgtgg ctcgcctcgt tcggagcgca gcgttggttc gcgccgttca taggctggcg
     1261 cagtggctca ctcccccgcg ccgtcgcaga ccacgaccac ccccggccag cagcggctcg
     1321 acggcaccca acgagccctc gccgccggcc tgctgctgtg cgtcacgctc gtcgcgttcg
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnacggcct gtcgctgtac ggcgtcacct
     1441 acgccgtgta catgctggcc gacctggtcg cgctgcagtg gagcagcgag acagccgacc
     1501 gctacggggt gcgccggccg tttctggtgt cggtcgctct gttcgtcatc ggcctgctca
     1561 tcgccgcctc ggcaccgacc atggagctgg tcgtgctcgg ccgtggcctg cagggggcag
     1621 gaagcggcgg gttcgcgccg ttgacgttca tcacggtgca gcgggccttt ccggagagcc
     1681 gccgggcgtc gctgttcgcg ctgctcagtg cgggatgggt gctgccgacg ttgatcgcac
     1741 cggcgatctc cgggttggtc accgaccatt tcggttggcg gttggtgttc ctgggcatct
     1801 tgccgatcgc gctcgcagtc gccaccgtca ccagccgggc gatggtcgcc gtgccgccac
     1861 ccgacctcgc cgtcgagcgc cgcgccagcc tgctgcccaa ctcggcccgc actgcgatcg
     1921 gcatcgccat ggtcgcgttc ggagcacagt ttgcccagtg gtggctggcc gccggcctgt
     1981 gcatcagcgg ctcggcgatc gccgccaaac cgctgcgcag cctcttgccg aagggcttct
     2041 tccgcgctcg ctccgggctg gccgcgatca tggtcgtgcg cttcctgtcg acggcgatgt
     2101 tcttgggcgt cgacagcttc gtgcccctcg ccgccgaccg cctgcacggt gcgcgcccga
     2161 tcgtccaggg cttcaccatc gcgggtgcgg cggtgctgtg gagtttgggc caagcgtttg
     2221 ccgcccgccg cggtacccgc ctgctgccca gccgtggagc ggcaatcggg ttcgcgttga
     2281 tgctgatcgg ggccggagcc accgtgccgg tgatcgcccc gtcgtggccg ctgtgggcca
     2341 cgttcatcag ctggagtgtc gccggcttcg gcatgggcat catcttcaac cccaccaccg
     2401 tcagcgcgat gacctacgcc gacgacagcc gcgaaggcga gatcggcggc caactgcact
     2461 tggccgactc gctcgggttc gggtgcatga gcatcctcgg cggggccacc gtcgcgatcg
     2521 ccgaccggac ctcgttcagc ctgcagggcg cgctcggcac caacttctcg atggccctca
     2581 tctgcggcgt gatcggcctg atcgtcgccc accgtgtgca cgcccgcacc acctgagcca
     2641 gcacagcgaa caccgcacga ccgttgcggg aaaaccccga tgtggcatcg gtcaccgata
     2701 cctaccatcg cggccatgag cgagaccgac aaccaacacg acgccactcg tcacgagaac
     2761 gacgatgccc tcatcgtcgc gatcgagcgc gtcgcggccg agtcggggtt cagcggtgcg
     2821 ctgcgcatcg accgccgtga ccgaccgccg atcgcgctcg ccnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngctgtccc accgctcggg
     3061 catcggcgac tacctggacg aagaggcgta cgagatcacc gactacgtga tgaggcgccc
     3121 ggtgcacgag ttggacacca ccgaggcgtt cctaccactg ctcgacggtt tcccgatggt
     3181 ggccgtgccc ggtgagcagt tcgcgtacaa caacggtgcg ttcgtggtgc tcgcgctgct
     3241 gatcgagaga gcgaccgggc agagctatca cgacgtggtg cnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nacccgccca
     3541 cggagccact cggccgagga ggaggcgcgg tacgggctgg gattctggct gggtgaatac
     3601 gacgacagca tcgagctgcg cggctacgac gccggggtgt cgtttcacgc cgtccaccaa
     3661 cccagcacgg ggacgaccta ctcgatcatc gccaactgga ccgatggcgc gtggcctctg
     3721 gcccggctgc tgacggctac gttcgcaccg gctgcctgac ccggctgccc gcacctggtt
     3781 ccacccgtgt cgtcagcccg ccaagatcgc gcgggcgacg aggtcggtac cgaaggcggt
     3841 gaggatcgcc agatacaaca acccggtcgc agccatgacc gcggagtagt acttctcggt
     3901 cagcgctcgt tgcgtcaccc agcacagggc ccacgtggtg accgcgcagg ccacccagaa
     3961 ccagccgagc atgccgcccc agccgtcgtc gaccgacccg c
//
LOCUS       DAORNF010000268         3981 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_16954_length_3981_cov_1.789098, whole genome shotgun sequence.
ACCESSION   DAORNF010000268 DAORNF010000000
VERSION     DAORNF010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3981
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16954_length_3981_cov_1.789098"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="PLV68_11200"
     CDS             <1..314
                     /locus_tag="PLV68_11200"
                     /inference="COORDINATES: protein motif:HMM:NF036525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01857.1"
                     /translation="RTSDYASAHPRIVSEVPVNVRDQHMAEQRAYGDHWQGLLDDAQH
                     AGLVDAATDLFLVRLLLFSAMNGLAEWHRPTSDADVRRLANETADLLLDGLISTGGTH
                     R"
     gene            311..709
                     /locus_tag="PLV68_11205"
     CDS             311..709
                     /locus_tag="PLV68_11205"
                     /inference="COORDINATES: protein motif:HMM:NF024092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HRE01858.1"
                     /translation="MTSPANLDAALAAMSAASRGDHEAQLALYTSDAVVEVVLGGRRF
                     AGTDELRTVFRMAAEQFPIEITIDEIVPCADPDQLVLEMHADGVVQATGKTFVKRYIS
                     RFWFRDGAISRQREFFDPAAMAAAMQPDPM"
     gene            727..876
                     /locus_tag="PLV68_11210"
     CDS             727..876
                     /locus_tag="PLV68_11210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01859.1"
                     /translation="MSDTNQIGKRYQCATCGLTVIVAKKGAGRVSCHGTAMEMLTNKP
                     LPSSD"
     gene            complement(1106..1909)
                     /locus_tag="PLV68_11215"
     CDS             complement(1106..1909)
                     /locus_tag="PLV68_11215"
                     /inference="COORDINATES: protein motif:HMM:NF025050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRE01860.1"
                     /translation="MTSTRIHRVTVYANDLDRAADDFAAAFAVGMTRADHPRLGVPAA
                     LSEAVSIVGRTPHSAPGPLAAVELTTPDIGAASAALTERGLAPEHHADGTLWVDFHGL
                     PFVVTGPAEPVEAAGATRFGRAVLAVDDVDAAVADLAAVFGVSLQAFDVENMRIRVAL
                     GEEGIEFIGKLDPQIDIEQLWHWPISGYAVHVDDLDAAKGRMAAAGAVLSYEFITPGG
                     LPEVYYGRPGLHGVPITLMPEHDAGGLLASMGVDGEVGSVAPTMTPGSD"
     gene            complement(1906..2268)
                     /locus_tag="PLV68_11220"
     CDS             complement(1906..2268)
                     /locus_tag="PLV68_11220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01861.1"
                     /translation="MTATHVMVVLADPAPGRDAELDEWYADHLRRMSAVDGVVAVRRL
                     VPVTGFGSDPGHRSIALYELRTDDLTSTVAMLAAARATFPATDAFARDPVVWFLEPAA
                     IDVPRPDVPRPAVIGDQP"
     gene            complement(2265..3314)
                     /locus_tag="PLV68_11225"
     CDS             complement(2265..3314)
                     /locus_tag="PLV68_11225"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRE01862.1"
                     /translation="MLTAEPPAVEVATGDSPAVTVAGRARRSAALGAGALVSLVVLIA
                     ALYSWVGLTGNVRLFVALGSAALAARLLWAAARTCWPHVDLGLRSSAAWLIVLVTAAV
                     FAGLLPLAEARDPSKTLTGPTLLRPDLFSGHPFGTDRIALDLLGGVLYGARVSMVVGL
                     GAAAVGLLVGLPVGMAAGYLRGGFEVVIDFATNVMLAFPPIILLLAMVSVMRPSIVNV
                     AVALGVISIPIFVRIAKLNTTTFASREFVTAAVAVGASRRRVLVRELLPCVLPPVLTY
                     GVLVVAVAIVAEASLSFLGLSIQRPEPTWGNMIAAGRDSYTTNPHPVLVVTVLALNNV
                     NQALRKRFDAQDAKL"
     gene            complement(3301..>3516)
                     /locus_tag="PLV68_11230"
     CDS             complement(3301..>3516)
                     /locus_tag="PLV68_11230"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRE01863.1"
                     /translation="SAGRLLGGAVVVETIFSLPGMGSLMVNAVANKDFRMVQGAVLIV
                     AVFYVVINGVVDVVSTTLDPKSRHAHR"
     assembly_gap    3517..3596
                     /estimated_length=80
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3597..3808)
                     /locus_tag="PLV68_11235"
     CDS             complement(<3597..3808)
                     /locus_tag="PLV68_11235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01864.1"
                     /translation="MLALVAWRFWPAFPRQGFSRLTSDKGLRENLRSVFLPALTLSLA
                     EIPVLTAVLRSDAINTLQQDYILFARS"
BASE COUNT          685 a         1374 c         1320 g          522 t
ORIGIN      
        1 tgcgcacgag cgactacgcg tcggcccatc cccgcatcgt cagcgaggtg ccggtgaacg
       61 tgcgcgatca gcacatggcc gaacagcgcg cctacggcga tcactggcag ggtctgctcg
      121 acgacgctca gcacgcgggt ctcgtcgatg ccgcgaccga cctgttcctc gtccggctgc
      181 tgctgttctc ggccatgaac ggtcttgccg agtggcaccg gcccacctcg gatgccgatg
      241 tccgacgcct cgccaacgag acggccgacc tgctgctcga cggcctcatc agcaccggag
      301 gcacccaccg atgacctcac ccgccaacct cgacgccgcg ctcgccgcca tgagcgccgc
      361 cagccgcggc gaccacgagg cccaactcgc gctgtacacc tccgacgccg tcgtcgaggt
      421 ggtgctcggg ggccgccgtt tcgccggcac cgacgagctg cggacggtgt tccggatggc
      481 cgccgagcag ttcccgatcg agatcacgat cgacgagatc gtgccctgcg ccgatcccga
      541 ccagctcgtg ctggagatgc acgccgacgg tgtggtgcag gccaccggca agacgttcgt
      601 caagcgctac atcagccggt tctggttccg cgacggggcc atcagccgcc agcgggagtt
      661 cttcgatccg gcggcgatgg ccgccgcgat gcaacccgac ccaatgtgat tggagatgcc
      721 gtcgtcatga gcgataccaa ccagatcggc aagcgttacc agtgcgcgac atgcggcctg
      781 accgtgatcg tcgccaagaa gggtgccggc cgggtgagct gccatggcac cgcgatggaa
      841 atgttgacca acaagccgct gccgtcgtcg gactgacccg cccggcacca acgaaaccag
      901 tgagcgagga gacacgatga gcgagatcaa gaccggcacg cggcttcgct gcgagcagtg
      961 ttcgaccgag gtgatcgtgg tcaccggcgg cgatgccgaa ctgacctgtt gcgaccagcc
     1021 catggtcgtg ctcgggtcgg gcccgtcctg gcggctgagc gcaggcgtca accgctccga
     1081 acgccgcggc ccggagcctc ccgcctcagt cggatccggg cgtcatcgtc ggcgcgaccg
     1141 acccgacctc gccgtcgacc cccatcgagg cgagcaatcc accggcgtca tgctcgggca
     1201 tcagcgtgat cggcacgccg tgcaggcccg gacggccgta atacacctcg ggcaagccac
     1261 cgggggtgat gaactcgtag ctgagcaccg cgcccgcggc cgccatccgg cccttggccg
     1321 cgtccaggtc gtcgacgtgc accgcatagc cgctgatcgg ccaatgccag agctgctcga
     1381 tgtcgatctg cgggtcgagc ttgccgatga actcgatgcc ctcctcaccc agcgcgaccc
     1441 ggatccgcat gttctccacg tcgaaggcct gcaacgacac gccgaacacc gcggccaggt
     1501 cggcgaccgc ggcatcgaca tcgtcgaccg ccaacacggc gcggccgaac cgcgtcgccc
     1561 ccgccgcctc gacgggctcg gccggccccg tcaccacgaa cggcagcccg tggaagtcga
     1621 cccacaacgt gccgtcggcg tgatgctcag gggcgagacc acgctcggtc agcgcggcgc
     1681 tggcggcacc gatgtcgggg gtggtcaact cgaccgcggc gagcggcccg ggtgccgagt
     1741 gtggggtgcg accgacgatg gagacggcct cgctcagcgc ggcaggcacg cccaagcgtg
     1801 gatggtcagc gcgggtcatg ccgacggcga aggccgcggc gaagtcgtcc gcggcgcgat
     1861 cgagatcgtt cgcgtacacg gtgacacggt ggatgcgggt cgaggtcatg gctggtctcc
     1921 gattacggct ggtcgtggta cgtctggtcg tggtacgtcg atggcggcgg gctcgaggaa
     1981 ccacacgacg gggtcgcgcg cgaatgcgtc ggtggcgggg aacgtggcgc gagccgcggc
     2041 caacatcgcc acagtcgagg tgaggtcgtc ggtgcgcagc tcatagagcg cgatcgaacg
     2101 atgacccggg tcggacccga acccggtcac gggaaccagc cggcgcaccg ccaccacacc
     2161 gtccaccgcg gacatgcgcc ggagatgatc ggcataccac tcgtcgagct cggcgtcgcg
     2221 acccggtgcc ggatcggcga gcaccaccat cacgtgcgtc gcggtcacag cttggcgtcc
     2281 tgggcgtcga agcgcttgcg cagcgcctgg ttgacgttgt tcagcgccag cacggtcacc
     2341 accaacacgg ggtgggggtt ggtggtgtag ctgtcgcgac ccgcagcgat catgttgccc
     2401 cacgtcggtt cgggccgctg gatgctgagg ccgaggaagc tcaacgaggc ctcggccacg
     2461 atcgccacgg ccaccaccag cacgccgtag gtcagcaccg gcggcaacac gcacggcaac
     2521 agttcgcgga cgagcaccct ccgccggctc gccccgacgg ccacggccgc ggtgacgaac
     2581 tcccggctgg cgaacgtggt cgtgttcagc ttggcgatgc ggacgaagat cgggatcgag
     2641 atcaccccga gcgccaccgc gacgttgacg atgctcggtc gcatcacgct gaccatcgcc
     2701 aacagcagga tgatcggcgg gaatgcgagc atgacgttgg tcgcgaagtc gatcaccacc
     2761 tcgaacccac cgcgcagata gccagcggcc atgccgaccg gcagaccgac gagcagtccc
     2821 actgcggcag caccgagacc gaccaccatc gacacccgcg cgccgtacag caccccaccg
     2881 agcaggtcga gggcgatccg gtcggtgccg aacggatgcc ccgagaagag gtctgggcgc
     2941 agcagcgtcg gacccgtcaa tgtcttcgac gggtcgcggg cctcggccaa cggcaacagg
     3001 ccggcgaaga cggccgctgt caccagcacg atcagccatg ccgcgctcga tcgcaacccg
     3061 agatcgacgt gcggccagca cgtgcgggcc gccgcccaca gcagccgggc agccagcgcg
     3121 gcgctgccca acgccacgaa caaccggacg ttgccggtga gacccaccca cgagtacagc
     3181 gcagcgatca gcacgaccag gctcaccagc gcgcctgcac cgagtgcagc gctgcgccgg
     3241 gcgcgaccag ccaccgtcac cgccgggctg tccccagtcg ccacctcgac ggcaggaggt
     3301 tcagcggtga gcatgacggc tcttcgggtc gagggtggtc gacacgacgt cgacgacgcc
     3361 gttgatcacc acgtagaaga cggcgacgat cagcacggcg ccttgcacca tgcgaaagtc
     3421 cttgttggcg acggcgttga ccatcagact gcccatgccg ggcaggctga agatcgtctc
     3481 caccaccacc gccccaccca acaggcgtcc agcagannnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngaac
     3601 gggcgaacag gatgtagtcc tgctgcagcg tgttgatcgc gtcggaacgg agcacggcgg
     3661 tgagcacggg gatctccgcc aggctcaagg tcagtgcggg caggaacacc gagcgcaggt
     3721 tctcgcgcag acctttgtcg gaggtcaacc gggagaagcc ctggcgagga aacgccggcc
     3781 agaaccgcca tgcgacgagc gcgagcacca ggcaggtggc cgcgccggtg acggtggacg
     3841 gccaggtggt cggcgtcgtc ggctacgacg tccagatcgt cacccgcacg ctgccggcca
     3901 cgctggtcac gctcgtggtc ggcgtgtcct gcttcagcgc cctgggcctg gcggcggccg
     3961 accgccgccg ggatgaggtc c
//
LOCUS       DAORNF010000269         3977 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_16977_length_3977_cov_1.472463, whole genome shotgun sequence.
ACCESSION   DAORNF010000269 DAORNF010000000
VERSION     DAORNF010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3977
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16977_length_3977_cov_1.472463"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>346)
                     /locus_tag="PLV68_11240"
     CDS             complement(<1..>346)
                     /locus_tag="PLV68_11240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01865.1"
                     /translation="THFSVFRRSLVDEVGGFRAGFEGSQDYDLILRVTERARYIHHFT
                     WIMYLWRQLPTSVAGDRNAKPYAYESGRRALQEHADRLGMDVTVEMQEPLGTYRFRRN
                     LTRTPKVSIVIPT"
     assembly_gap    347..475
                     /estimated_length=129
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<476..783)
                     /locus_tag="PLV68_11245"
     CDS             complement(<476..783)
                     /locus_tag="PLV68_11245"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRE01866.1"
                     /translation="MSPRISVLTPVYNPPADVLQATIDSVRNQSYQDWELCLVDDCST
                     EAHVRQVLDAAAASDARIRVQYRSENGGIIPASQDALDMATGEFVALLGPSGCGKSTL
                     L"
     gene            complement(780..1145)
                     /locus_tag="PLV68_11250"
     CDS             complement(780..1145)
                     /locus_tag="PLV68_11250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01867.1"
                     /translation="MPTKTPAAAAKAGSSTASLTAAERAELADLRTEVARLRELVGPS
                     EDSYAKLKTDVWGARDAVIGAEAECGILRGRVRSLEAELTRALKEQQWFKHEVINRLR
                     SYRVIHRKVIGRVRRTLVK"
     gene            complement(1157..1909)
                     /locus_tag="PLV68_11255"
     CDS             complement(1157..1909)
                     /locus_tag="PLV68_11255"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRE01868.1"
                     /translation="MSAETVIGSGAGVLVVVPAYNEEDCIADVVRAVIAAGHRCLVVS
                     DASTDRTVARANDAGATTIALPINLGVGGALRCGWRYAHDRGEQVVVQVDGDGQHPID
                     SIGELIAVAERDGLDMVIGSRFAHGGSHEGVSWFRRRCMNLLSRILRRSAGVTLSDPT
                     SGFRAITQPLLGEFAESFPHHFLGDTFEAELVAGRRGYRIGEIAIAMRARQGGTPSAD
                     IYASGRAMLRALVVLVTGTSFDVRSRQSMPRR"
     gene            complement(1899..2318)
                     /locus_tag="PLV68_11260"
     CDS             complement(1899..2318)
                     /locus_tag="PLV68_11260"
                     /inference="COORDINATES: protein motif:HMM:NF021575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2304 family protein"
                     /protein_id="HRE01869.1"
                     /translation="MSTAAKQALVLAGGGLLLAIVILRLARRRLLTTRYAFGWLGVGV
                     LVVAAAAALPFVGRLGRIANITPTAVLLLMSSAVLLAISIQLSISVSLLQERLRATAE
                     AHALLARRVEDLEAELAATAVAAAAVHVPMTSGVDER"
     gene            2426..>2609
                     /locus_tag="PLV68_11265"
     CDS             2426..>2609
                     /locus_tag="PLV68_11265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01870.1"
                     /translation="MSALFRKKQTDAPVADAVAPTQLPSARTDPESAAPSPPGQPGQP
                     GQPDTLAERRILAAPCP"
     assembly_gap    2610..2644
                     /estimated_length=35
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2691..>2995
                     /locus_tag="PLV68_11270"
     CDS             2691..>2995
                     /locus_tag="PLV68_11270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01871.1"
                     /translation="MPLFDDQRIRTILANLDSIKNTKVLELAPFEGGHTYLLHEAGAS
                     VQAIEERTHEYLRCLVVKEILNLNRAHFRLGNYRPYLAETADRFDMVLCSGILHEQT"
     assembly_gap    2996..3118
                     /estimated_length=123
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3119..3322
                     /locus_tag="PLV68_11275"
     CDS             <3119..3322
                     /locus_tag="PLV68_11275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01872.1"
                     /translation="VDFRGRHINLHPILAAGADGTRSVFARWIERDSLLAVLAALGFG
                     SIRVLDDQIDHPAGPSILLIAEA"
     assembly_gap    3346..3487
                     /estimated_length=142
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3488..>3977)
                     /locus_tag="PLV68_11280"
     CDS             complement(<3488..>3977)
                     /locus_tag="PLV68_11280"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRE01873.1"
                     /translation="AVVDRLAVVENGIDASATVAGKYPHNRRVVAVGRLVDQKGFDVL
                     IRAFSGVVRDVPDAELVIVGDGHRRGELERLVAAGGVMDRVEMCGALPHADIAGLLAS
                     AAVVAMPSRFEGLPLVALEASLAGRPLVATPVQGLGDVVVDGVTGLVVPVEDDAALAA
                     ALV"
BASE COUNT          610 a         1265 c         1137 g          536 t
ORIGIN      
        1 gtggggatga cgatgctgac cttgggtgta cgggtgaggt tgcgccggaa gcggtaggtg
       61 ccgagcggct cctgcatctc gaccgtgacg tccatgccca accgatcggc gtgctcttgc
      121 agcgcgcgtc gaccggactc gtaggcgtag ggcttggcgt tgcgatcgcc cgccaccgac
      181 gtcggcagtt gacgccacag atacatgatc cacgtgaagt ggtggatgta tcgcgctcgt
      241 tcggtgacac gcaggatcag gtcgtagtcc tggctgccct cgaacccggc acggaagccg
      301 ccgacctcgt cgaccaggct gcgcctgaag acggagaagt gggtgcnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnagcaa
      481 ggtcgatttt ccgcagccgc ttggccccag caaggcgacg aactcgcccg tggccatgtc
      541 gagcgcatcc tgcgaggcgg ggatgatgcc gccgttctcg ctgcggtact gcacccggat
      601 gcgggcatcg ctggctgcgg cggcatcgag cacctggcgc acgtgcgctt cggtggagca
      661 gtcgtcgacc agacacagct cccagtcctg gtaactctgg ttgcgcaccg agtcgatcgt
      721 ggcctgcaac acgtcggccg gcgggttgta gaccggcgtc agaacggaga tgcgcgggct
      781 catttgacga gcgtgcgccg cacgcggccg atcaccttgc gatggatcac ccggtacgag
      841 cggagccggt tgatgacctc gtgcttgaac cactgctgtt ctttgagggc gcgggtgagt
      901 tcggcttcga gcgatcgcac ccgtccacgg aggatgccgc actcggcctc ggccccgatc
      961 accgcgtcgc gagcgcccca cacgtcggtc ttcaacttgg cgtaggagtc ttcgctcggc
     1021 ccgaccagct cacgcagccg ggccacctcg gtgcgcaggt cggcgagctc ggcccgttcg
     1081 gcggcggtca gcgaagcggt gctgctgcca gccttggcgg cggccgcggg ggtcttggtc
     1141 ggcatggccc gacaggttac cggcgcggca tcgactgccg ggagcgcacg tcgaagctgg
     1201 tgcccgtgac caagaccacc aacgcgcgca gcatcgcccg ccccgacgcg tagatgtcgg
     1261 ccgacggtgt gccgccctgg cgagctcgca tcgcgatcgc gatctcgccg atgcgataac
     1321 cgcgtcggcc cgcgaccagc tccgcttcga acgtgtcacc caggaagtga tgtgggaacg
     1381 actccgcgaa ctcacccaag agcggctggg tgatggcgcg gaaccccgac gtggggtcgg
     1441 acagcgtcac cccggcgctg cgccgcagga tgcgtgacag caggttcatg cagcgccgcc
     1501 ggaaccacga caccccctcg tgcgaacccc cgtgagcgaa gcggctaccg atcaccatgt
     1561 cgagcccgtc acgctcggcg accgcgatca gctcaccgat cgagtcgatc gggtgttggc
     1621 cgtcgccgtc gacctggacg accacctgct cgccgcggtc gtgggcgtag cgccatccac
     1681 agcgcagtgc cccaccgacg ccgaggttga tcggcaacgc gatcgtggtt gcgcctgcgt
     1741 cgttggcgcg agccaccgtg cggtcggtgc tggcgtcgct gaccacgagg cagcgatgac
     1801 cggccgcgat caccgcgcgg acgacgtcgg cgatgcagtc ctcctcgttg tacgcaggaa
     1861 cgacgaccag cacgcccgcg ccagacccga tgaccgtctc agcgctcatc gacaccggag
     1921 gtcatcggta cgtgcacggc agcggctgcg accgcggtcg cggcgagttc ggcttccagg
     1981 tcctcgaccc ggcgagccag cagggcgtgc gcttcggccg tggcccgcag ccgctcttgc
     2041 agcaacgaca cgctgatcga aagttggatc gagatggcca acaacaccgc cgagctcatc
     2101 agcagcaaca cggcggtcgg ggtgatgttg gcgatacgtc cgaggcgtcc gacgaacggc
     2161 agcgcggccg ctgcggcgac gaccagcacg cccacgccga gccagccgaa cgcatacctc
     2221 gtcgtgagca ggcgacgccg ggcgagacgc aggatgacga tcgcgaggag caatccccca
     2281 ccggccagca ccagagcctg cttcgctgcc gtcgacatgc gcggaatgct acccgtggct
     2341 gtttccctgg tcgccggtca cgggttgacg ggcatagagt tcccgcgccg atgcgcgtct
     2401 gcgctgtccc acaaatcgag tcgccatgtc cgccctgttc cgcaagaagc agaccgatgc
     2461 cccggtcgcc gacgccgttg ctccgaccca gctcccctca gctcgaacag accccgagtc
     2521 ggccgcgccc agtccgcccg gtcagcccgg tcagcccggt cagcccgaca cgctggccga
     2581 acgccgcatc cttgccgcgc cgtgtccacn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnatggaa gtctgcgctg ccatcgatcc ttggtttgaa cgcaggcgag atgccgctgt
     2701 tcgacgacca gcgcatccgc accatcctcg ccaacctcga cagcatcaag aacaccaagg
     2761 tgctcgaact cgcgccgttc gagggtggcc acacatatct gctgcacgag gcgggggcca
     2821 gcgtgcaggc catcgaggaa cgcacacacg aatacctgcg ctgcttggtg gtgaaggaga
     2881 tcctcaacct gaaccgggct cacttccgtc tgggcaacta ccggccatat ctggccgaga
     2941 ccgccgaccg gttcgacatg gtgctgtgca gcggcatcct ccacgaacag acggannnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngt
     3121 cgacttccgc ggccgccaca tcaacctcca cccgatcctg gccgccggcg ctgacggcac
     3181 ccgatcggtg ttcgcccgct ggatcgagcg cgactcgttg ctcgccgtgc tcgctgcgtt
     3241 gggtttcggc tccatccggg tcctcgacga ccagatcgac caccccgccg gccccagcat
     3301 cctgctgatc gccgaggcct gagcaagcag ctcaggacac ctgcgnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnncac cagtgccgcg gccagagccg catcgtcctc gaccggtacg accagaccgg
     3541 tgacgccatc gaccacgaca tcgccaaggc cctgcaccgg tgtcgccacc aatggtcgcc
     3601 cggccaacga cgcctccaac gccaccaacg gcaaaccttc gaaacgcgac ggcatggcga
     3661 caaccgccgc cgacgccaag agcccggcta tgtccgcatg aggaagcgcg ccgcacatct
     3721 cgacgcggtc catgacacca cctgctgcaa ccaaacgctc aagctcaccg cggcggtggc
     3781 cgtcaccgac gatcaccaat tcggcatccg gcacgtcgcg caccacgccc gagaacgcgc
     3841 ggatcagcac gtcgaagccc ttttggtcga cgagtcggcc gacggcgacc acccggcgat
     3901 tgtgcgggta cttccccgcc accgtcgccg atgcatcgat gccgttctcg accacggcga
     3961 ggcgatctac aacagct
//
LOCUS       DAORNF010000270         3959 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_17091_length_3959_cov_1.682377, whole genome shotgun sequence.
ACCESSION   DAORNF010000270 DAORNF010000000
VERSION     DAORNF010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3959
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17091_length_3959_cov_1.682377"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="PLV68_11285"
     CDS             <1..373
                     /locus_tag="PLV68_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HRE01874.1"
                     /translation="AFCLRGGRARVQGIGEVLTPLEPVRQDITLVIPPLSCSTPAVYR
                     AWDALGGPTGDGVNDLEPAALTIEPRLADWRDRIHAAVGIVPSLAGSGATWFLHGHHP
                     GLADALDSSGAVVVLTVTSGA"
     gene            complement(471..551)
                     /locus_tag="PLV68_11290"
     CDS             complement(471..551)
                     /locus_tag="PLV68_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003402602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AURKAIP1/COX24 domain-containing protein"
                     /protein_id="HRE01875.1"
                     /translation="MKKRRKRMRKKKHKKMLRRTRHQRRK"
     assembly_gap    553..653
                     /estimated_length=101
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            694..767
                     /locus_tag="PLV68_11295"
     tRNA            694..767
                     /locus_tag="PLV68_11295"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:726..728,aa:Gln,seq:ttg)
     gene            795..1799
                     /locus_tag="PLV68_11300"
     CDS             795..1799
                     /locus_tag="PLV68_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HRE01876.1"
                     /translation="MAGGLVGLFDDIAALAKLAAASIDDVGAAAGKASVKAAGVVVDD
                     TAVTPAYVHGLAAERELPIIKRIAKGSIRNKVLFILPAALVLSQFLPIAVEIILMCGG
                     AYLSYEGAHKIHHRLSHHASSDHSGGGHAADGDAGAGGGEHGAPAAVPAALTDQEIEN
                     KTVASAVRTDFILSAEIMVIALKEVIDEGFVSRSIILLIVAALITAMVYGVVALIVKM
                     DDMGLALAEREDPRAKRFGRGLVAAMPKVLAVLSSVGTVAMLWVGGHILVAGCHELGW
                     HGPRSLIHAIEDPVHHLAVVGGIAAWFADTALSAVVGLIVGFALVVAVTRLTARRTRT
                     "
     gene            1868..>2361
                     /locus_tag="PLV68_11305"
     CDS             1868..>2361
                     /locus_tag="PLV68_11305"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HRE01877.1"
                     /translation="MSVSAIVLAAGEGTRMRSTRPKPLHMICGRSMVMHVIHALEAVT
                     PDRTVVVVGHGAEQVTKKVHEQSPGWANVTFVEQHVQRGTGDAATIGLTAFPGDDLDD
                     ESTVLVLPGDTPLLRADTIDELVATHVANRCAATVLTSVMDDPTGYGRIVRGEGKTGA
                     QRGVR"
     assembly_gap    2362..2385
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2386..2965
                     /locus_tag="PLV68_11310"
     CDS             <2386..2965
                     /locus_tag="PLV68_11310"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HRE01878.1"
                     /translation="AERAIAEINTGIYAFRRDLLGPALRRLSPDNSQGEYYLTDVISV
                     LAGMGHRIGAMLAPPDETAGVNDRWQLALAERELRARTNRHWLLNGVTMLDPRQTFID
                     VTVELGRDVTLFPGTMLQGSTSVGNGSEIGPNTRLTDCVVGRDCVVEHTVGIDAEIGD
                     NARVGPFAHLPAGSAVISETTTGAFYTASTEA"
     gene            3002..>3631
                     /gene="prs"
                     /locus_tag="PLV68_11315"
     CDS             3002..>3631
                     /gene="prs"
                     /locus_tag="PLV68_11315"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HRE01879.1"
                     /translation="MEKVTTKRLALYSGRTHPDLADEVAKHLDLVLGDPNIVEFANGE
                     IRPRFVESVRGSDVFIMQTHFGCNGRSINDSIMEQLIMIDAAHRASAKRITAVCPFYG
                     YARQDRKAEGREPITARMVADMFRAAGAKRMVSIDLHSGQIQGFFEGPVDHLTALPVL
                     ERYVRENGSPGMVIVSPDAGRVKVAERFAQHLSDLGSLLQALQQRGEARS"
     assembly_gap    3632..3743
                     /estimated_length=112
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3744..>3959
                     /locus_tag="PLV68_11320"
     CDS             <3744..>3959
                     /locus_tag="PLV68_11320"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: protein motif:HMM:NF025923.2"
                     /note="catalyzes the formation of 5-phospho-alpha-D-ribose
                     1-phosphate from D-ribose 5-phosphate and ATP; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HRE01880.1"
                     /translation="SRGASEVWVMATHGVLSGPAISRLRDSPFARVVLTNTLPLPSKA
                     ELPQLEVLSIAPIIAEALSAVFDDTSVS"
BASE COUNT          592 a         1326 c         1200 g          604 t
ORIGIN      
        1 cgccttctgc ttgcgtggtg gccgtgcccg cgtgcagggc atcggcgaag tgttgacccc
       61 gctcgaaccc gtgcggcagg acatcacctt ggtgatcccc ccgctgtcgt gctcgacacc
      121 ggcggtctac cgcgcctggg acgcgctcgg cggcccgacg ggcgacggcg tcaacgatct
      181 cgagcctgcc gccctcacca tcgagccacg gctggccgac tggcgcgatc gcatccacgc
      241 cgccgttggg atcgtgccct cactggccgg gtcgggcgcg acgtggttcc tgcacggcca
      301 tcaccccggt ctggccgacg cgttggacag ctcgggtgcg gtcgtggtgc tcaccgtgac
      361 ctcgggcgca tgatcagcat ctgaatgcag accgaaaaag cagaacagcc gcccaccgac
      421 ggcggacggc cgacgtacgc cgacgtgcgg cgcacactgc tgaaaatgaa ctacttgcga
      481 cgctggtgac gggtccgccg cagcatcttc ttgtgcttct tcttgcgcat gcgcttgcgg
      541 cgcttcttga tcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngagccga
      661 cgatccggcc cgataccgtg tgcccccgac cgttccgggg tcgttcaatg gtaggacagc
      721 ggtttttggt gccgtctata ggggttcgag tcctctcccc ggagccactc tctcggagac
      781 catcggagac gctcatggct ggaggcttgg tcggcctctt cgacgacatc gccgccctgg
      841 ccaagttggc cgctgcctcg atcgacgatg tcggcgcggc tgcgggcaag gccagtgtca
      901 aggcagccgg ggtcgtcgtc gacgacaccg ccgtgacgcc ggcgtacgtg cacggcctgg
      961 ccgccgagcg cgagctgccg atcatcaagc ggatcgccaa ggggtcgatc cgcaacaagg
     1021 tgctgttcat cctgcccgcg gcgctggtgc tgagtcagtt cctgccgatc gcggtggaga
     1081 tcatcctgat gtgcggcggg gcctacctca gctacgaagg cgcccacaaa atccatcatc
     1141 gcctcagtca ccacgccagc agcgatcact ccggcggcgg gcatgctgct gatggcgatg
     1201 ccggtgctgg tggcggcgag cacggggcgc ctgccgcggt cccggccgcg ctcaccgatc
     1261 aggagatcga gaacaagacc gtcgccagcg cggtgcgcac cgacttcatc ctgtccgccg
     1321 agatcatggt gatcgccctc aaagaggtca tcgacgaagg gttcgtgtcg cgcagcatca
     1381 tcctcctgat cgtcgctgcg ctgatcacgg ccatggtgta cggcgtggtc gcgctgatcg
     1441 tgaagatgga cgacatgggc ctcgccctgg ccgagcgcga agacccgcgc gcgaagcggt
     1501 tcggcagggg gctggtcgcg gcgatgccca aggtgctggc cgtgctgtcc agcgtcggca
     1561 ccgtggcgat gctgtgggtc ggcggtcaca tcctggtcgc gggttgccac gagctgggtt
     1621 ggcacgggcc acggtcgctg atccatgcga tcgaagaccc ggtgcaccac ctggccgtgg
     1681 tcggcggcat cgccgcctgg ttcgccgaca ccgcgctgtc ggccgtcgtc gggttgatcg
     1741 tcggcttcgc cttggtcgtc gccgtcaccc gtttgacagc acggcgcacg agaacctagt
     1801 catgtctctt ctgcgatcct gcccgcgccg gcacccacat cacggcacgg cgcgctagaa
     1861 cctagtcatg tccgtttccg caatcgtgct cgccgccggt gaaggcaccc gcatgcgatc
     1921 cacccggccc aagccgttgc acatgatctg cggccggtcg atggtgatgc acgtcatcca
     1981 cgcgctcgaa gccgtcaccc ccgaccgcac cgtggtcgtc gtcggccacg gcgccgaaca
     2041 ggtcaccaag aaggtgcatg aacagtcgcc cgggtgggcc aacgtgacct tcgtcgaaca
     2101 acatgtccaa cgcggcacgg gcgacgcggc caccatcggc ctcaccgcgt ttcccggcga
     2161 cgacctcgac gacgagtcga ccgtgctggt gctgcccggc gacaccccgt tgttgcgggc
     2221 cgacaccatc gacgagctcg ttgccaccca cgtcgccaac cgctgcgccg ccacggtgct
     2281 gacgtcggtg atggacgacc ccaccggcta cggacggatc gtgcgcggcg agggcaagac
     2341 cggcgcgcag cggggggtgc gnnnnnnnnn nnnnnnnnnn nnnnncgccg agcgagcgat
     2401 tgccgagatc aacaccggca tctacgcctt ccgccgcgat ctgctcggcc ccgcgctgcg
     2461 ccgcctgtca cccgacaaca gccagggcga gtactacctg accgacgtga tcagcgtgct
     2521 ggccgggatg ggtcaccgca tcggcgcgat gctggccccg ccggacgaga ccgcgggggt
     2581 caacgaccgc tggcaactgg ctctggccga gcgcgaactg cgcgcccgca ccaaccgcca
     2641 ttggctgctc aacggcgtca ccatgctcga tccgcgccag accttcatcg acgtcacggt
     2701 cgagctcggc cgcgacgtca ccctcttccc tggcaccatg ctgcagggtt caacatcggt
     2761 cggcaacggc agcgagatcg gccccaacac ccgcctgacc gactgcgtgg tcgggcgcga
     2821 ttgcgtggtc gaacacaccg tcggcatcga cgccgagatc ggcgacaacg cccgggtcgg
     2881 accgttcgcc cacctgcccg ctggtagcgc cgtcatttcg gagacgacga ccggcgcctt
     2941 ctacactgcc agtaccgagg cgtgacacgg cacccccacg cgtcggcccg taggagcgct
     3001 catggagaag gtcaccacca aacgtctggc gttgtactcg gggcgcacac accctgatct
     3061 ggccgacgag gttgccaagc atctcgactt ggtcttggga gaccccaata tcgtcgaatt
     3121 cgccaacggc gagatccgtc cccgcttcgt cgagagcgtg cgcggcagcg acgtgttcat
     3181 catgcagacc cacttcgggt gcaacgggcg cagcatcaac gactcgatca tggaacaact
     3241 gatcatgatc gacgccgccc accgcgcctc ggccaagcgg atcaccgcgg tctgcccctt
     3301 ctacggctac gcccgtcaag accgcaaggc cgaaggccgt gaaccgatca ccgcccgcat
     3361 ggtggccgac atgttccgcg ccgccggtgc caagcggatg gtgtcgatcg atctccacag
     3421 tggccagatc cagggcttct tcgaaggccc ggtcgatcac ctcaccgcgc tgccggtgct
     3481 cgagcgctac gtccgcgaga acggctcacc gggcatggtg atcgtgtcgc ccgacgccgg
     3541 acgggtcaag gtcgccgaac gcttcgccca gcacctcagc gatctcgggt cgctcctcca
     3601 ggcgctgcag cagcgcggcg aagcgcggtc gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnagccgcg gcgcgtccga ggtgtgggtc atggccaccc
     3781 acggcgtgct ctccggcccg gcgatcagcc gcctgcgcga ctcgcccttc gcccgcgtgg
     3841 tcctcaccaa caccctgccg ctgcccagca aggccgagct accccaactc gaggtgctgt
     3901 cgatcgcccc gatcatcgcc gaggcgttgt cggccgtgtt cgacgacacc agcgtcagc
//
LOCUS       DAORNF010000271         3954 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_17124_length_3954_cov_1.648884, whole genome shotgun sequence.
ACCESSION   DAORNF010000271 DAORNF010000000
VERSION     DAORNF010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3954
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17124_length_3954_cov_1.648884"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>319
                     /locus_tag="PLV68_11325"
     CDS             <1..>319
                     /locus_tag="PLV68_11325"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HRE01881.1"
                     /translation="PLYIWLPDAMAGPTPVSALIHAATMVTSGVFLLTRLAPLLHYGE
                     PWMMTVIAVVGLLTALFAATIAVAQNDIKKVLAYSTVSQIGFMVLAVGVGNYVGAIFH
                     LMTH"
     assembly_gap    320..438
                     /estimated_length=119
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <439..>1206
                     /locus_tag="PLV68_11330"
     CDS             <439..>1206
                     /locus_tag="PLV68_11330"
                     /inference="COORDINATES: protein motif:HMM:NF013198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HRE01882.1"
                     /translation="FIIGWLAIAGVPPFAGFWSKDEILLFAYDKSPILWALALVAALL
                     TAYYMTRQVIMVFFGKARWNDPIDADADDHADDHGHHVEPHESPATMTLPLVALAGLA
                     AVGGVLNLPFSGVKEKLLHWLEPLVHHAERELSHGSEDIKWVLAGVATAVAVTGIVLA
                     YVVYQKRSIKAIEPTVLAKGWYYDSAITDFMGGPGRDAFEGVAWFDANVIDGAVNGTG
                     KSVQGLAGVLRKAQNGLVRTYAALIGVGVVLLLVWFL"
     assembly_gap    1207..1264
                     /estimated_length=58
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1265..>1679
                     /locus_tag="PLV68_11335"
     CDS             <1265..>1679
                     /locus_tag="PLV68_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Fe-S-binding domain-containing protein"
                     /protein_id="HRE01883.1"
                     /translation="PILTMLILVPAFGSLAVAVLTKRRRELVKLVAVLTSVFTAALGV
                     WMLASFHTGEEGFQFVSRHEWISAWGISWHVGVDGISLFLVVLTAVLFPLAIVGADPH
                     HDEKPYMAWMLMLEAGVIGSFLSLDLFLFFIFFEIV"
     assembly_gap    1680..1740
                     /estimated_length=61
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1741..>2699
                     /locus_tag="PLV68_11340"
     CDS             <1741..>2699
                     /locus_tag="PLV68_11340"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HRE01884.1"
                     /translation="TKFFLYTMFGSAFMLVGIIATVLLAKDDIGHVTFDLVEIATGAN
                     FAASTGRWLFFAFAIAFAVKVPLFPLHTWLPDAHTQSPTAGSVVLAGVMLKLGTYGLI
                     RFGVYLFPSAAYTTRAIFLTLAVIGIIYGAIAATMQRDLKRVVAYSSIAHLGFIVLGT
                     FAFTNQSLTGGIVQMVNHGVSTGALFLLVGMIYERRHTREIAALKGIQSVAPIFAAAF
                     TLVMLSSIGVPGLNGFVGEFLVLVGSFPTARWWTVVAASGVILAALYLLWAYQRVFHG
                     EPDDDNRSFAELRAKEALVLAPFLIAIVFTGIYPKPMLDRIEP"
     assembly_gap    2700..2739
                     /estimated_length=40
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2795..>2997
                     /locus_tag="PLV68_11345"
     CDS             2795..>2997
                     /locus_tag="PLV68_11345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01885.1"
                     /translation="MLPEFTAPAIEWFALSPLLIMLGAGLVLLVVGALTPPWPKRLYA
                     LTTAAAALAAMITTFCVWDNVSD"
     assembly_gap    2998..3101
                     /estimated_length=104
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3102..>3498
                     /locus_tag="PLV68_11350"
     CDS             <3102..>3498
                     /locus_tag="PLV68_11350"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HRE01886.1"
                     /translation="GMDGPEVYAMLLMAAIGGAVMVMANDMIVLFLGLEVLSISLYVL
                     AASHRRARGSQESGIKYFILGSFSSAVFLYGIALIYGGTGSTNFSKIIAAFDRVPGER
                     KDALVLIGVALMMVGLLFKATAAPFHFWAP"
     assembly_gap    3499..3574
                     /estimated_length=76
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3586..>3954
                     /locus_tag="PLV68_11355"
     CDS             3586..>3954
                     /locus_tag="PLV68_11355"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HRE01887.1"
                     /translation="MALPNWRDDWRPVIWAVAALTLATGAVMAVVQKNVKRMLAYSAI
                     SHAGFILLGVEAAGHSGGSTGPGMPASMLYLLLYSVLVIGTFAVVSVVSGVGDEATSL
                     DAFNGLAKRRPVLAVALTVFL"
BASE COUNT          489 a         1148 c         1153 g          706 t
ORIGIN      
        1 gccgctgtac atctggctgc ccgacgccat ggcgggtccc acgccggtgt cggcgctgat
       61 ccacgccgcc accatggtga ccagcggcgt gttcctgctc acccgcctgg cccccctgct
      121 gcactacggc gagccgtgga tgatgacggt catcgccgtg gtcggcctgc tcacagcgct
      181 gttcgcggcc accatcgccg tggcccagaa cgacatcaag aaggtgctcg cctactccac
      241 ggtgagtcaa atcggcttca tggtcctcgc cgtcggcgtc ggcaactacg tcggggcgat
      301 cttccacctg atgacgcacn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnngt tcatcatcgg ctggctggcc atcgccggcg tgccgccctt
      481 cgccgggttc tggagcaagg acgagatctt gctgttcgcc tacgacaaga gcccgatcct
      541 gtgggcgctc gcgctggtgg cggcgttgct gaccgcctac tacatgaccc gtcaggtgat
      601 catggtgttc ttcggcaagg cccgctggaa cgacccgatc gacgcagatg ctgacgatca
      661 cgccgacgat cacggtcatc acgtcgaacc gcacgagagc cccgcgacga tgacgttgcc
      721 gctggtcgcg ctggccggtc tggccgcggt cggtggtgtg ctcaacctgc ccttctccgg
      781 ggtgaaggag aagctcttgc actggctcga accgttggtg caccacgccg aacgcgagct
      841 cagccacggc tccgaagaca tcaagtgggt gctcgccggc gtcgccaccg cggtcgccgt
      901 gactggcatc gtgctcgcct acgtcgtcta ccagaagcgc tcgatcaagg cgatcgaacc
      961 gacggtgctg gccaagggtt ggtactacga ctcggcgatc accgacttca tgggcggccc
     1021 tggccgtgac gcgttcgaag gcgtggcctg gttcgacgcc aacgtcatcg acggagccgt
     1081 caacggcacc ggcaagagcg tgcagggtct ggccggggtg ctgcgcaagg cgcagaacgg
     1141 tctggtgcgc acctatgccg ccctcatcgg cgtcggtgtc gtgctgctct tggtgtggtt
     1201 cttgatnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnncccgat cctgacgatg ttgatcctgg tgccggcgtt cggttcgctg gcggtcgcgg
     1321 tgctcaccaa gcggcgccgc gaactggtca agctggtggc ggtgctgacc agcgtgttca
     1381 ccgccgccct cggcgtgtgg atgctggcct cgttccacac gggcgaagaa gggttccagt
     1441 tcgtcagccg ccacgagtgg atcagcgcgt ggggcatcag ttggcacgtc ggtgtcgacg
     1501 gcatctcgct gttcttggtg gtgctgaccg cggtgctgtt cccgctggcc atcgtcgggg
     1561 ccgacccgca ccacgacgag aagccgtaca tggcgtggat gttgatgctc gaagcgggcg
     1621 tgatcggcag cttcctgtcg ctcgacctgt tcctcttctt catcttcttc gagatcgtgn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 accaagttct tcctgtacac gatgttcggc tcggcgttca tgctcgtcgg catcatcgcc
     1801 acggtgttgc tggccaagga cgacatcggg cacgtcacgt tcgatctggt cgagatcgca
     1861 accggcgcca acttcgccgc atccaccggg cgatggctgt tcttcgcgtt cgcgatcgcg
     1921 ttcgcggtca aggtgccgct gttcccgctg cacacctggc tgcccgacgc gcacacccag
     1981 tcgccgaccg cgggctcggt ggtgcttgcg ggcgtgatgt tgaagctggg tacctacggt
     2041 ctgatccgct tcggcgtgta cctgttcccg tcggcggcct acaccacccg ggcgatcttc
     2101 ttgacgctgg cggtgatcgg catcatctat ggcgccatcg cggccacgat gcagcgcgat
     2161 ctcaaacggg tggtggcata ttcgtccatc gcccacctcg gcttcatcgt gctcggcacc
     2221 tttgccttca ccaaccagtc gctgactggc ggcatcgtgc agatggtcaa ccatggcgtg
     2281 tccaccggcg ccctgttctt gctggtcggc atgatctacg agcggcgtca cacccgtgag
     2341 atcgcagcac tgaagggcat ccagtcggtg gcccccatct tcgccgcggc gttcacgttg
     2401 gtgatgttgt cgtccatcgg tgtgcccggg ctcaacggct tcgtcggcga gttcttggtg
     2461 ctggtcggct cgttccccac cgcgcggtgg tggacggtgg tggccgcaag cggtgtgatc
     2521 ttggccgctc tgtacctgtt gtgggcctac cagcgggtgt tccacggcga gcccgacgac
     2581 gacaaccgca gcttcgccga actgcgcgcc aaggaagcgc tggtgctggc gccgttcttg
     2641 atcgcgatcg tgttcaccgg tatctacccc aagcccatgc tcgaccgcat cgaacccagn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna cagagactac gtcgcgcccg
     2761 ccgtcggtga cccgaccgag gcggaggcgc cctgatgctg cccgagttca ctgcccccgc
     2821 catcgagtgg ttcgcgctca gcccgctgct gatcatgctc ggcgccggat tggtgctgct
     2881 ggtcgtcggc gcgctgacac cgccgtggcc caagcggctc tacgccctca ccaccgcggc
     2941 cgcggcgctg gcggcgatga tcaccacgtt ctgcgtgtgg gacaacgtca gcgacgannn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nggcatggac ggtcccgagg
     3121 tctacgcgat gctgctcatg gccgccatcg gtggtgcggt gatggtcatg gccaacgaca
     3181 tgatcgtgct gttcctcggt ctcgaagtgc tgtcgatctc gctgtacgtg ctggccgcga
     3241 gccaccgccg ggcgcggggc agccaggaga gcggcatcaa gtacttcatc ctcggcagct
     3301 tctcgtcggc ggtgttcctg tacggcatcg ccctgatcta cggcggcacg gggtcgacca
     3361 acttctccaa gatcatcgcc gccttcgacc gggtgcccgg cgagcgcaag gacgcgctgg
     3421 tgctgatcgg cgtggccttg atgatggtcg gtctgctgtt caaggccacc gccgcgccat
     3481 tccacttctg ggcacccgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngagtgc tggtcgtggc cctgccgaac
     3601 tggcgtgacg actggcggcc ggtgatctgg gccgttgcgg cgctgacgct ggcgaccggt
     3661 gcggtgatgg cggtggtgca gaagaacgtc aagcggatgc tcgcctactc ggccatcagc
     3721 catgccgggt tcatcctgct cggtgtcgaa gccgccgggc acagtggtgg ttcgaccggg
     3781 ccgggcatgc cggcttcgat gctgtacctg ctgctctact cggtgttggt gatcggcacc
     3841 tttgccgtcg tcagcgtggt gtcgggggtt ggcgacgagg cgacctcgct cgacgccttc
     3901 aacgggttgg ccaagcgccg tccggtgctg gccgtggccc tcacggtgtt cctg
//
LOCUS       DAORNF010000272         3941 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_17203_length_3941_cov_1.742666, whole genome shotgun sequence.
ACCESSION   DAORNF010000272 DAORNF010000000
VERSION     DAORNF010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3941
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17203_length_3941_cov_1.742666"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(393..974)
                     /locus_tag="PLV68_11360"
     CDS             complement(393..974)
                     /locus_tag="PLV68_11360"
                     /inference="COORDINATES: protein motif:HMM:TIGR03086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03086 family metal-binding protein"
                     /protein_id="HRE01888.1"
                     /translation="MITDIITQIHQAIDATAVVVHAITEDQWHLPTPCADWDVRAVAN
                     HLVGGMLAFPELLAGVTVDDDPTREHVGTDPASAFDAAARADHDAWSRPDALDGTVAL
                     TFATLPASMAAVIHLTELVVHGLDLAVATGQHDTIDEHLVEQLLDLMHSMGGIDAFRS
                     PGFFGPEVHVNTAQPAHQRLLAYVGRNITCVAA"
     gene            complement(1021..>1328)
                     /locus_tag="PLV68_11365"
     CDS             complement(1021..>1328)
                     /locus_tag="PLV68_11365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRE01889.1"
                     /translation="EMLAITAAIPNAEAAPAIVNLVLLPLTFISGIWMVLTNAPTWLD
                     TIAKVLPVRAFAHGLQHAFHPATTGTGIVATDLMVLAVWTLAGLRICQRWFRWETRR"
     assembly_gap    1329..1376
                     /estimated_length=48
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1377..1850)
                     /locus_tag="PLV68_11370"
     CDS             complement(<1377..1850)
                     /locus_tag="PLV68_11370"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRE01890.1"
                     /translation="MNTTTTLVSSRRLDVTTSPSSRRRDLRLAVRQIGFEQRAFWRNR
                     ARAFFSVGLPLMFLVVFNALNGSHHIDELGGITYATWFVPGILAYGLMMATFSNLAIS
                     ITVSRDTGVLKRLRGTSLPTWVYIAARVGSTVITAATLVAATLGLGATAYGVHIRL"
     gene            complement(1847..>2262)
                     /locus_tag="PLV68_11375"
     CDS             complement(1847..>2262)
                     /locus_tag="PLV68_11375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRE01891.1"
                     /translation="SMIRSLAQLGKTVLLTTHFMDEAQALADRIAVVAGGRIIAEGTP
                     ETLGGRDRQHTVIETTLPGGIRADQLPDFGNASVELRDRTLRIEAADGIRATHTLTDW
                     ALSTAITLGRLSVSQPSLEDIYLTLTNSHTPELAR"
     assembly_gap    2263..2299
                     /estimated_length=37
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2300..2763)
                     /locus_tag="PLV68_11380"
     CDS             complement(<2300..2763)
                     /locus_tag="PLV68_11380"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRE01892.1"
                     /translation="MSPAIEVHHLAKTYGTFVAVHDISFTVERGEVMCLLGPNGAGKT
                     TTTEILEGYRDRTSGEVRVLGFDPGRHERAFRERIGIVLQECGVQDDLTVGEVLDMYG
                     TYAPQRLATADVIRLVELDAKVDDRVRHLSGGQRRRLDVGLAIVGDPEVIFL"
     gene            2890..3459
                     /locus_tag="PLV68_11385"
     CDS             2890..3459
                     /locus_tag="PLV68_11385"
                     /inference="COORDINATES: protein motif:HMM:NF021886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HRE01893.1"
                     /translation="MSLRHALLGLLADKPASGYELTKQFELSLANVWTASHSQIYPEL
                     QRMAERGWVVAGNEGARGRREYELTDDGRAELHRWLTGDRPDPSNRNEAMLRVFFLWT
                     LDRDEAVTFLEREADAYRDGLTALERLDAAVPWDDSGSDQMGRIALEQGLRWSKTMTE
                     WATWAADQLRAGHDATTLTDRQRRPSKRH"
     gene            complement(3739..>3941)
                     /locus_tag="PLV68_11390"
     CDS             complement(3739..>3941)
                     /locus_tag="PLV68_11390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HRE01894.1"
                     /translation="PFEIDIIRPDTFLLDQLDLAPGVVIDELGRQAAANRKDPKTLPS
                     ILDALAKAGAPSFADEVRRRIA"
BASE COUNT          665 a         1160 c         1390 g          641 t
ORIGIN      
        1 gtgatcgaga tcaagcacta ccccgacgcc gccgacttcg agcaccagcc gacgaccgga
       61 atcgatgact gaggaaccga ccccagaccg cagcaccacc acgacccggg cctcgccacc
      121 gcgttgaccg ctcggtcgac tcggatcgtg cgagtctcga caatgcagac caaccggcca
      181 cctctgccca ggtctgcggg ctactggaga gggtgcctac gagcgcctcg cttccacggg
      241 gtccacgatc agagcgagcg agcggtggtc atcgacggaa cccccgacga ccgcacctcg
      301 ctcaccgagt tcgagcacgg ccacgcggag tcaggcgccc cggagtcagg cccctcgctc
      361 gggccgtttc cgcttcgcgt cctggcgcgt gatcacgcag cgacgcacgt gatgttcctt
      421 cccacgtagg caagcaggcg ctggtgcgct ggctgggcgg tattgacgtg gacttcgggg
      481 ccgaagaagc cggggctgcg gaaggcgtcg ataccgccca tgctgtgcat cagatcgagg
      541 agttgctcga cgaggtgctc gtcgatcgtg tcgtgctggc ccgtggcgac ggcgaggtcg
      601 agcccgtgga cgaccagttc ggtgaggtgg atcaccgccg ccatcgacgc tgggagcgtg
      661 gcaaaggtga gcgcgacggt gccgtcgagt gcgtccggtc gactccaggc gtcgtggtcg
      721 gcgcgggccg cggcgtcgaa agcgctcgca gggtcggtac cgacgtgctc gcgcgtcgga
      781 tcgtcgtcga cggtgacgcc ggcgagcaac tcgggaaagg cgagcatgcc gccgacgagg
      841 tggttggcga cggcgcgcac atcccagtcg gcgcacggtg tggggaggtg ccactggtct
      901 tcggtgatgg cgtggacgac gacggcggtg gcgtcgatgg cttgatggat ctgggtgatg
      961 atgtcggtga tcatgatgtt gagtcctttc gagagcgggt gatcagggtg gtgttggcgt
     1021 tcagcggcgg gtttcccagc ggaaccagcg ttggcagata cgcagcccgg caagggtcca
     1081 gacggcgagg accatcaggt cggtagcgac gatgccggtg ccggtggtgg cggggtggaa
     1141 ggcgtgttgg aggccgtggg caaaggcgcg gacggggaga accttggcga tggtgtcgag
     1201 ccaggtaggg gcgttggtga ggaccatcca gattccggag atgaacgtga gcgggagcag
     1261 gacgaggttg acgatggcgg gtgctgcttc ggcgttgggg atggcggcgg tgatcgcgag
     1321 catctcgcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnaagg
     1381 cggatgtgta cgccgtaagc ggtggcgccg aggccgagcg tggccgcgac gagggtggcg
     1441 gcggtgatga cggtcgaacc gacacgtgcc gcgatgtaga cccatgtggg cagtgaggtg
     1501 ccgcggagtc gcttgaggac gccggtgtcg cgggagacgg tgatgctgat ggcaaggttg
     1561 ctgaacgtgg ccatcatcag gccgtaggcg aggatgcccg gcacgaacca agtggcgtac
     1621 gtgatgccgc cgagctcgtc gatgtggtgg ctgccgttga gggcgttgaa gacgacgagg
     1681 aacatgagcg ggagaccgac gctgaagaag gcccgggcgc ggttgcgcca gaacgcgcgc
     1741 tgttcgaagc cgatctggcg gacggcgagg cgaagatcgc gccgccgaga cgacggcgag
     1801 gtggtgacat cgaggcgacg actgctgacg agggtggtgg tcgtgttcat cgcgcgagct
     1861 ccggggtgtg cgagttggtc agggtgaggt agatgtcttc gagcgagggt tggctgacgg
     1921 agagccggcc gagggtgatg gcggtggaca atgcccaatc ggtgagggtg tgggtggcgc
     1981 ggataccgtc ggccgcttcg atgcgcaggg tgcggtcgcg caactccacg gaggcgttgc
     2041 cgaagtcggg caactgatcg gcacggatgc cgccgggaag ggtggtttcg atcaccgtgt
     2101 gctggcggtc gcgaccgccg agcgtctcgg gtgtgccttc ggcgatgatc cgtccgccgg
     2161 caacgacggc gatccgatcg gcgagcgcct gtgcctcgtc catgaaatgg gtggtcagca
     2221 ggacggtctt gccgagctgg gcgagcgagc ggatcatcga ccnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnt cgaggaagat cacctctggg tcgccgacga tggcgagccc
     2341 cacgtcgagt cgtcggcgct gaccgccgga aaggtgccgc acgcggtcgt cgaccttcgc
     2401 gtcgagttcg acgagtcgga tcacgtcagc cgtcgcgagt cgctgtggcg cgtaggtgcc
     2461 gtacatgtcg agcacttcgc cgacggtcag gtcgtcttgc actccgcact cttgcaggac
     2521 gatgccgatt cgttcgcgga acgcgcgctc gtgacggccg ggatcgaacc cgaggacgcg
     2581 cacctcaccg ctcgtgcggt cgcgatagcc ctcgaggatc tcggtagtgg tggtcttgcc
     2641 ggcgccgttc gggccgagca ggcacatcac ttcaccgcgc tcgacggtga aactgatgtc
     2701 gtggacggcg acgaacgtgc cgtaggtctt ggcgaggtgg tgtacctcga tcgccgggct
     2761 catggttgct ccacttcggt cgcggtcggc gctgtaatgc gttcgatcct ctgacgtgtc
     2821 gatcttgaca tgtcagaggt ttaatatgtc tgactcgaca tgtcaagacc gacttgttag
     2881 catgtggcca tgagcctgcg acatgcactc ctcgggctgc tcgccgacaa accggccagc
     2941 ggttacgagc tgaccaaaca gttcgagctg tccctcgcca acgtgtggac cgcgagccac
     3001 tcgcagatct accccgagct gcaacggatg gccgaacgcg gttgggtcgt cgccggcaac
     3061 gaaggagcac gcggacgacg cgaatacgaa ctcaccgacg acggtcgcgc cgaactgcac
     3121 cgctggctca ccggcgaccg ccccgatccc tccaaccgca acgaagcgat gctgcgcgtg
     3181 ttcttcctct ggactctcga ccgcgatgag gcggtgacgt tcctcgagcg tgaagccgac
     3241 gcctaccgtg acggcctcac cgcactcgaa cgactcgacg ccgccgtccc gtgggacgac
     3301 agcggcagcg accagatggg acggatcgcc ctcgaacaag gcctccgatg gtcaaagacg
     3361 atgaccgaat gggccacctg ggccgccgac caactccgcg ccggccatga cgccaccact
     3421 ctcaccgacc gccaacgacg ccccagcaag cgccactgag tctccctcac tcgatgacat
     3481 ccggaggagc ctggtagacg ggtcccgcga ccgtcgggcc tcggccgaag acttcaggca
     3541 cccagcggca tcggccgcca tcacggacac cagcggtcac tgccgggtca tgttcacgcc
     3601 acgacgtgtc gccgcctgct cggctggctg cggtctctgc ctgccaccct cgatgctgac
     3661 gcggttgggg agatctacgc caaggcgagg ctcagcagca cctggaagta acggccgctc
     3721 acttccggcc gctaccgctc atgcaatacg tcggcgaacc tcgtcagcga acgacggagc
     3781 acccgccttc gcgagtgcat cgaggatgct tggaagagtc ttcgggtctt tgcggtttgc
     3841 ggccgcttgt cgaccaagct catcgatgac gactccaggt gccagatcga gttgatcgag
     3901 caggaaagtg tccgggcgaa tgatgtcgat ctcgaacggg t
//
LOCUS       DAORNF010000273         3935 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_17230_length_3935_cov_1.365206, whole genome shotgun sequence.
ACCESSION   DAORNF010000273 DAORNF010000000
VERSION     DAORNF010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3935
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17230_length_3935_cov_1.365206"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="PLV68_11395"
     CDS             <1..180
                     /locus_tag="PLV68_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRE01895.1"
                     /translation="DMTAALGDKRIAELTAAVPLGRMATPDEIAGVVAFLASDDAAYV
                     TGAVIPVDGGLGMGH"
     assembly_gap    246..443
                     /estimated_length=198
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            525..812
                     /locus_tag="PLV68_11400"
     CDS             525..812
                     /locus_tag="PLV68_11400"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HRE01896.1"
                     /translation="MQGLGRRVAITGFGVVAPCGVGKDAFWQGLLGPGLTGTKAVQFA
                     EWDPSPYYDSPKEARRADAVEQFATASAIEAIDGISSITSSVRLVMAMWKA"
     gene            800..1813
                     /locus_tag="PLV68_11405"
     CDS             800..1813
                     /locus_tag="PLV68_11405"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HRE01897.1"
                     /translation="MEGIVDLRRFGVVYGTGIGGLRTLEEQVEIRLAKGEKRVSPFLV
                     PMMMGNASGAAISMRYGLQGPLETICTACAAATHAIGYAARLIAWGRCDAMVTGGSEA
                     AATPTSIGGFNNMTALSTSGASKPFDVTRDGFVMGEGAATFILEEWSVAEARGATILG
                     EVLGAASTADAHHITAPSPGGVGAIHCIELALADAGLQPGDIAHINAHGTSTPLNDAA
                     EAQVIACASAPVGGLLADFAGWRVALMALAVFGAGTPPVTSTKGVTGHALGAAGALEA
                     AAVLLAMQHRLIPPTAGTTELSPDMHIDLVMGAPRPWTPGPSISNNFGFGGHNGSVII
                     GPG"
     assembly_gap    1910..1922
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1923..>2218)
                     /locus_tag="PLV68_11410"
     CDS             complement(<1923..>2218)
                     /locus_tag="PLV68_11410"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ family
                     protein"
                     /protein_id="HRE01898.1"
                     /translation="DELVSLEPGLRATGLWRLTGDEWFFAGHFPGRPTLPGVLMCEAI
                     AQVGAIAVLTDQRFAGKLPLFGGLDGARFRRQVSPGDTLELSVELGRMSARAGK"
     assembly_gap    2219..2334
                     /estimated_length=116
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2335..2733)
                     /gene="bcp"
                     /locus_tag="PLV68_11415"
     CDS             complement(<2335..2733)
                     /gene="bcp"
                     /locus_tag="PLV68_11415"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HRE01899.1"
                     /translation="MLTPGDIAPTAALLDQDANPFDWTALSGHRTLLFFYPKASTPGC
                     TQQACGLRDVVGQIGDTTIIGVSPDKPKAQKNFATKYELPYPLLADSDHELAEAYGVW
                     KEKKNYGRTYMGIERSAFLIGADGRIEQAWY"
     gene            complement(3390..3701)
                     /locus_tag="PLV68_11420"
     CDS             complement(3390..3701)
                     /locus_tag="PLV68_11420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01900.1"
                     /translation="MSASEGCELCEAARITPWYRDDELCWIAECEFCDVPMVVLRHHT
                     ATPGAAVRRLLHQRLIEAVLSRPGTAEAGSNEFASSWWIDDNMRNIPSHYHAHARRRR
                     G"
     gene            complement(3710..>3935)
                     /locus_tag="PLV68_11425"
     CDS             complement(3710..>3935)
                     /locus_tag="PLV68_11425"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HRE01901.1"
                     /translation="LAESATRLAGRLKQITDVPVLVGVGVSNAAQAREAAAVADGVIQ
                     GASVVRRLMDGGPDAVGAYVAEVRAALDQV"
BASE COUNT          605 a         1252 c         1152 g          599 t
ORIGIN      
        1 gacatgaccg ccgcgctcgg cgacaagcgc atcgccgaac tgaccgctgc cgtaccgctg
       61 ggacgcatgg ccacgcccga cgagatcgcc ggagtggtcg cgttcctggc cagcgacgat
      121 gcggcatatg tcacgggggc cgtgataccc gtcgacggcg gcttgggtat gggacactga
      181 gcactcgttc cgttcggcat cccatccggc atcattgaca ccgttctcac tcacaccaac
      241 cctcgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnncggcgtc gaggtgcccg aagaggagct ggacggcatc
      481 gagaccgtcg gccaggcgtt cgacctggtc gtcggcaagc tctgatgcag ggactcggtc
      541 ggcgcgtcgc catcaccgga ttcggtgtgg tcgcgccgtg cggggttggc aaggacgcct
      601 tctggcaggg gctgctcgga cctggtctca ccgggaccaa ggccgtgcag tttgccgagt
      661 gggacccttc gccatattac gacagcccga aagaggcgcg ccgcgccgac gctgtcgaac
      721 agttcgccac cgcgtcggcg atcgaagcca tcgacgggat ctcgtccatc acctcgtcgg
      781 tcaggttggt gatggcgatg tggaaggcat agtcgatctg cgccggttcg gcgtcgtcta
      841 cggcactggc atcggtggat tgcgcacact ggaagaacag gtggagatcc gcctcgccaa
      901 gggtgagaag cgagtctcgc cgtttctcgt gccgatgatg atgggcaacg cgtccggtgc
      961 tgcgatctcg atgcgctacg gcctgcaagg gccgctggag acgatctgca ccgcgtgcgc
     1021 ggccgccacc catgccatcg gctacgccgc ccgcctcatc gcctggggcc gttgcgacgc
     1081 gatggtcacc ggtggttccg aagcagcagc cacgccgacc tcgatcggtg ggttcaacaa
     1141 catgaccgcg ctgtccacca gcggtgcctc caagccgttc gacgtcaccc gagacggctt
     1201 cgtgatgggc gaaggcgcgg ccaccttcat cctcgaagag tggagcgtgg ccgaagcccg
     1261 cggggccacg atcctgggcg aagtcctcgg cgcggccagc accgccgacg ctcaccacat
     1321 caccgcgccg tcacccggcg gcgtgggcgc cattcactgc atcgagttgg cattggcaga
     1381 cgctggtctg cagcccggcg acatcgccca catcaatgcc cacggcacct cgacaccgct
     1441 caacgacgcg gccgaggccc aagtgatcgc ctgcgccagc gcaccggttg gcggcctgct
     1501 ggccgacttc gccggctggc gcgtggcgct gatggcgctg gccgtgttcg gcgccggcac
     1561 tcccccggtc accagtacca agggtgtgac cggtcacgcc ttgggagctg ccggtgcgct
     1621 cgaggcagcc gcggtgctgc tggcgatgca gcaccgcctc atcccgccca ccgcgggcac
     1681 caccgagttg tcgcccgaca tgcacatcga cctggtgatg ggtgcgcccc ggccgtggac
     1741 accgggcccg tcgatctcga acaacttcgg cttcggcggc cacaacgggt cggtgatcat
     1801 cggcccaggc tgatcaccct gaccggtcac ccagacaggt cagttcaggg ccgtcgcctc
     1861 aggcgtcgac gagcacgaac atcaactcgc actcacaggc aagggcaccn nnnnnnnnnn
     1921 nnccttgccc gcgcgagcgg acatccgacc gagttcgacc gagagctcga gcgtgtcgcc
     1981 ggggctcacc tggcgcctga agcgggcgcc gtcgagtccg ccgaacaatg gcagcttgcc
     2041 tgcgaagcgc tgatcggtca gcaccgcgat cgcgccgacc tgggcgattg cctcgcacat
     2101 caacacacct ggcagcgtcg ggcgcccggg gaagtgcccg gcgaagaacc actcgtcacc
     2161 cgtgagacgc cacagccccg tggcgcgcag accgggttcc aggctgacca gttcgtcgnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngtacca
     2341 cgcttgctcg atccgcccat cggcgccgat caggaacgcc gagcgctcga tgcccatgta
     2401 cgtgcgcccg tagttcttct tctccttcca cacgccgtag gcctcagcca gttcgtggtc
     2461 gctgtcggcc agcaagggat agggaagctc gtacttggtg gcgaagttct tctgggcctt
     2521 cggtttgtcg gggctgaccc cgatgatcgt ggtgtcgccg atctggccga cgacgtcgcg
     2581 taggccgcac gcctgttggg tgcatcccgg cgtcgacgcc ttggggtaga agaacagcag
     2641 cgtgcgatga ccagagagag ccgtccagtc gaaggggttg gcgtcttggt cgagcagggc
     2701 cgccgtgggg gcgatatcac cgggagtgag catgggcacg accttagttg gccgcgctgt
     2761 gcgttctccg cgagtcaggc ggatgtcagg gcaatgcaaa ggaatctcac cgtgcgtgat
     2821 ggcgtgaccg tgcgtcgtcc actttgacca aatctttccg atcgctcacc ttgtcagtcg
     2881 cacaatcccg tggtacaaat atcgcacctg acaaggcgcg atgccttggt cgacccggag
     2941 aggagaggga gcaatgcgcc ccatcatcgt tcgcccctgc tgagattcgc cactctgtgt
     3001 gttgtcgtac tcactgacag aaaaccaccc aacgtggcat cctgccgaac gagcacacgg
     3061 catccgtccc ctccgcaacc ttcttcgaat cagaaagaac caccacgatg cgtcccatta
     3121 tcgtccgtcc ctgctgagcg ccgaccgcga tccttccccc cattgagaga tgctccagaa
     3181 aggaccctgg caatgcgccc gatcatcgtt cgcccctgct gatcaccgaa tcgtgaccct
     3241 catcgtctga gagaccggcc ccggccgcta cagacataga caagcgaccc gtcgagcccg
     3301 ccaatccgtg gcgagaagct ccgggtctct ttttcgcacc tcagttcaca ggacgtgtga
     3361 gcagcggccc gcctctgccc agatgtgcgt caaccccgac gtcgtcgggc gtgggcgtga
     3421 tagtggctgg ggatgttgcg catgttgtcg tcgatccacc acgacgatgc gaactcgttg
     3481 ctccccgctt cggctgtgcc ggggcgcgac aacacggcct cgatcaagcg ctgatgcaac
     3541 agccgccgca ccgcggcacc gggggtggcg gtgtggtgcc gcagcaccac catcggcacg
     3601 tcgcagaatt cgcactcggc gatccagcac agctcgtcgt cgcggtacca cggcgtgatc
     3661 cgggccgctt cgcacagctc gcacccctcg gacgccgaca ccgcgcccgc tacacctgat
     3721 cgagcgcggc gcgcacctcg gcgacgtagg cgccgaccgc gtcgggaccg ccgtccatca
     3781 gccggcgcac gacgctggcc ccctggatga ccccgtccgc gaccgctgct gcctcgcgag
     3841 cctgggccgc gttggagacg cccactccga ccagcaccgg cacgtcggtg atctgcttca
     3901 gacggccggc cagtcgggtc gccgactcag ccagg
//
LOCUS       DAORNF010000274         3927 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_17281_length_3927_cov_1.683884, whole genome shotgun sequence.
ACCESSION   DAORNF010000274 DAORNF010000000
VERSION     DAORNF010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3927
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17281_length_3927_cov_1.683884"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..104
                     /locus_tag="PLV68_11430"
     CDS             <1..104
                     /locus_tag="PLV68_11430"
                     /inference="COORDINATES: protein motif:HMM:NF014621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0051499 - D-aminoacyl-tRNA deacylase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HRE01902.1"
                     /translation="LRRLGATVATGRFRTEMEVTLTNDGPVTVMVDI"
     gene            complement(174..410)
                     /locus_tag="PLV68_11435"
     CDS             complement(174..410)
                     /locus_tag="PLV68_11435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01903.1"
                     /translation="MALTCVPSPELNVLIRATARLVTDLAEVEQLWNDQVLPYDPGMF
                     FSGPSDPMTQFVELTPTMATIHPLGPGPWRRYTR"
     gene            1283..1708
                     /locus_tag="PLV68_11440"
     CDS             1283..1708
                     /locus_tag="PLV68_11440"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HRE01904.1"
                     /translation="MQHAWYVLAFDLDRSDWRTFRLDRIDGPISVPGHGFRHRPIPGG
                     DPTQFVSDAFALAPYEHSAHVEVDARPDAVAAAMPRLSRRRITARGTAQATVELSGSD
                     ADAVVRDLVDLCLYLTVESVTATDAIHARLARLVPSLTP"
     gene            1903..3114
                     /locus_tag="PLV68_11445"
     CDS             1903..3114
                     /locus_tag="PLV68_11445"
                     /inference="COORDINATES: protein motif:HMM:NF017470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HRE01905.1"
                     /translation="MDAPSCPRCGTSAAGGGWWSQPPTPERALALTPLAVADPAAIAV
                     PEVGSATAALRRKLGVAALVVVGIVALLRLQDAGYDGDYISGFDYLAHASPLSRAVLL
                     LGWGSLVAGALLLRKHGKPTPWPIVAVVALVLARHPDLPSWDRQNERSFAGASDREFF
                     LGNLFLLAGLVLSIMALQITAAGDSTPVPNAWVGQASPGQRQVAAPPGWLADPHRRAE
                     HRYWDGAMWTANVSTHGRQSTEPTPPTWAPPPTTTIDVTPSMAHPMAHGAASFATASY
                     GPAIHKPAKAGMGWYIRREFAQYATFSGRAGRAEYWWFFLLNMVISLAVAAIIAASGA
                     VAFAVVGGLWTLATMVPGIAVFVRRLHDTNRSGTHCLLGLIPFVGAIILLVLVSSAGT
                     PGPNRYGPPAP"
     gene            3189..>3927
                     /locus_tag="PLV68_11450"
     CDS             3189..>3927
                     /locus_tag="PLV68_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HRE01906.1"
                     /translation="MQQPPLDDLLKLLDLEAIEVNIFRGRSPDEDRQRVFGGQVAGQA
                     LVAASRTVDQPHRMVHSLHAYFLRPGDPTVPILYEVDRIRDGRSFTTRRVRAIQNGRA
                     IFNLQASFHVDERGPDHQIPMPSGVPDPETLPDFKTLMYPYREQMGQWYDRPRPIDLR
                     YVEADPIGRRGTPTDGQKVWLRADGVLPDDPTLHACIVTYASDMTLLDTAALPDPDLI
                     IRTSGELRVSNFLIWQGAYSEWFVTPKY"
BASE COUNT          582 a         1382 c         1337 g          626 t
ORIGIN      
        1 aactccgtcg cctcggagca acggttgcaa ccggccgctt tcgcaccgag atggaggtga
       61 ccctcaccaa cgacggcccg gtcaccgtga tggtcgacat ctgatcagcg ctccgtggag
      121 ggcgtaggtt gtggcaatgg cggcagtggt tcggtcgtgc tcgccagggt cggtcagcgg
      181 gtgtagcggc gccaggggcc ggggccgagt gggtggatgg tggccatggt cggggtcagt
      241 tcgacgaact gggtcatcgg gtcggacggg ccgctgaaga acatgccggg gtcatagggc
      301 agcacctggt cgttccagag ctgctcgacc tcggccaggt cggtgacgag gcgtgccgtg
      361 gcccggatca acacgttgag ttcgggcgac gggacacacg tcagcgccac gtcggcgcgc
      421 tggccggccg ccgtcgccct ggtcgtgcca aagccgctgg tcggcccatc cggccgacac
      481 gaaggcgacg tgtggggtgc cagcggcgtc ggccgtggcc aggaacaagc cgtcaccgcc
      541 agcttcgacg gcaccgcgca actcgctgaa gctgatcggg acaatggtcg ggcggggcat
      601 gtcgttggtg ctcatgccga cgaccgtacg cggcaatgcg gacaagatcg gtccgcatcg
      661 tgagaaactc tggcggatat gaccacaact gccctcatgg ccgaggactc gactgggcgg
      721 gccttcgcgc tgttgtcgat gctgaccgcg ggcacccatt ggcgagggtc cgacctggct
      781 gagcggctca gcgtgacgac ccgcacggtg cgccgcgacc gcagccaggg tgagatggtg
      841 cttctggtcg aggacaacga cgacgtgcgg gcggtcacgg cccgccgcct gcgccgcctc
      901 ggctaccgca tcgacgccgc accgggcgct gacggtggct accggttggt cggcggcacc
      961 gcgagtccgc cgttgttctt cgccgaggac gaagccgtcg ccgtcgtgac cgcgttgctc
     1021 gcgtcgatca gccgccagcc cgccaccacg gccgacgccg gactgcgggc gctggccaaa
     1081 ctgcgtcacg tccttccggc caaggctcag gccacggccg cagcggtgca gtcgagcgcg
     1141 cacatggtcg cggcgccgat tgccccgccc gtcgacccgc acagcatcgg cttgctcgcc
     1201 gggtgttgcg ccagcgcgca ggtcgcacgg ttcgcctacc tcgaccgcac gggcatcgcc
     1261 accgaacgac gcgctcgtca cggtgcagca tgcgtggtac gtgctcgcgt tcgatctcga
     1321 ccgctcggac tggcgcacct tccgcctcga ccgcatcgac ggaccgatca gcgtgccggg
     1381 ccacgggttc cgtcaccggc cgatccccgg tggcgatccg acccagttcg tgagcgacgc
     1441 cttcgctctc gcgccgtacg agcactcggc ccacgtcgag gtcgacgcgc ggccggatgc
     1501 ggtcgctgcc gccatgccgc ggttgagccg ccggcggatc accgctcgcg gtaccgcaca
     1561 agcaacggtc gaactgagcg ggtccgacgc cgatgccgtc gtccgcgacc tggtcgatct
     1621 gtgcctctac ctcaccgtcg agtcggtcac cgcgaccgat gcgatccacg cccgcctcgc
     1681 ccgcctcgtc ccgtccctca ccccatagca aaccccgccc cggtgaatct ggcgtccgcg
     1741 gatggtggag tggccgcgag tgctgaggag tggggagttg tccccatcga cagcgctgcg
     1801 gaggatggcc acactgaccc gcaccgcagg tgtgggacag gcaccggacg gcgcgggaag
     1861 gagcgtgcag tgaggtgtga gatgtgcagt accgaactcg ccgtggacgc accgtcgtgc
     1921 ccacgctgcg gtaccagtgc ggctggcggt ggctggtgga gccagccgcc gacgccagaa
     1981 cgggcattgg ccctgacccc cctggcagtc gctgacccgg cggctatcgc cgttcctgag
     2041 gtcggctctg cgactgccgc gctgcggcgc aagctcggtg tcgcggcact cgtggtcgtc
     2101 ggcatcgtcg cactcctacg cctgcaagac gcgggctacg acggtgacta catctcgggt
     2161 ttcgactacc tcgcccacgc ttctccgctc tcccgagcgg tgctgttgct cggatggggc
     2221 agcctggtcg ctggcgccct actgctgcgc aaacacggca agccgacgcc gtggccgatc
     2281 gtcgccgtcg tagcgctggt gttggcgcga catcctgacc ttccaagctg ggacaggcag
     2341 aacgagagat ccttcgcggg agcgtcggac cgcgagttct ttctgggcaa cctgttcctg
     2401 ttggcggggc tcgtgttgtc gatcatggcg ttgcagatca cggcggctgg cgacagcacg
     2461 cctgtgccca acgcatgggt cggtcaggcc agccctgggc agcggcaggt cgcggcaccg
     2521 ccggggtggt tggccgatcc gcaccggcga gccgagcacc gctactggga cggcgcgatg
     2581 tggacggcca acgtgtcgac ccacggccgc cagtcgaccg agccgacacc acccacctgg
     2641 gcgccgccgc cgaccaccac catcgacgtc acgccatcca tggctcaccc gatggctcac
     2701 ggtgcagcct cgttcgccac ggcgagctac ggcccagcga tccacaaacc tgccaaggcg
     2761 ggcatggggt ggtatatccg gcgcgagttc gcgcagtacg ccaccttctc cgggcgggcc
     2821 ggccgggccg agtactggtg gttcttcctc ctcaacatgg tgatctcctt ggcagtggcc
     2881 gcgatcatcg ccgcctccgg ggcggtcgcg ttcgcggtcg tcggcgggct ctggacgctg
     2941 gcgaccatgg tgccggggat cgcggtgttc gtccgccgcc ttcacgacac caaccgctcc
     3001 ggcacgcact gtctgctggg gttgatcccg ttcgtgggag cgatcatcct gctggtgctg
     3061 gtgtcctcgg ctggcacccc cggacccaat cggtacgggc cgccagcacc gtgacacacg
     3121 aggtgggccg cgcgggccgc ggcgatcggg ccgacgagga gaaggggtgg tggcagtaag
     3181 ttgtcgggat gcagcagcca ccgctcgacg accttctcaa gttgctcgac ctcgaagcga
     3241 tcgaggtcaa catcttccgt ggccgctctc ccgacgagga ccgccagcgc gtcttcggtg
     3301 gtcaggtcgc cggtcaggcg ttggtagccg cgagccgcac cgtcgaccaa ccccaccgca
     3361 tggtgcactc gctgcacgcc tacttcctgc gccccggcga ccccaccgtt ccgatcctgt
     3421 acgaggtcga ccgcatccgc gacgggcgca gcttcaccac ccggcgcgtc cgcgcgatcc
     3481 agaacgggcg ggcgatcttc aacctgcagg cgagcttcca cgtcgacgag cgcggccccg
     3541 atcaccagat cccgatgccg tccggcgtgc ccgaccccga gacgctgccc gacttcaaga
     3601 ccttgatgta cccctaccgc gagcagatgg gtcagtggta cgaccggccg cggccgatcg
     3661 acctgcgcta cgtcgaggcc gatccgatcg gccgccgcgg cacgccgacg gacggtcaga
     3721 aggtgtggct gcgcgccgac ggcgtgctgc ccgacgaccc gacgctgcac gcgtgcatcg
     3781 tcacctatgc cagcgacatg accctgctcg acaccgcggc gctgcccgac cccgacctca
     3841 tcatccgcac ctccggtgaa ttgcgcgtga gcaacttcct tatctggcag ggcgcctatt
     3901 ccgagtggtt cgttacgccg aaatatt
//
LOCUS       DAORNF010000275         3919 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_17325_length_3919_cov_1.605072, whole genome shotgun sequence.
ACCESSION   DAORNF010000275 DAORNF010000000
VERSION     DAORNF010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3919
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17325_length_3919_cov_1.605072"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1061)
                     /locus_tag="PLV68_11455"
     CDS             complement(<1..>1061)
                     /locus_tag="PLV68_11455"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HRE01907.1"
                     /translation="NVANWGQLTAEQSYADLFRTGTSPVAHFWSLAIEEQFYLVWPLL
                     WWGWARWVEPRLIDQFQPTARLALVWVLAMTAMTAAPLIARRWGANVVYLSTPARAGE
                     LLVGAVLAVMWRQRRAVGREAQRVLSIAGVGALGVVLGAMVLTPNGRGWPYSGGFPVF
                     AVIVSVLIAGAMVDGPLCRALAWRPLVAIGKVSYGIYLFHWPVFRLIDDAVLRVVVTA
                     AITLASYWLIERPVRRGGVRARRDLIGALVGIAVLVVATVAVVPGPTGPIGAVDADRA
                     AAVAIVPPPSSMPQQGTTPPSTTSERVPLDSTAPDNASPSSAAPVDVLSSEPEPLAPA
                     RVIVAGDSTALVLGSAMVD"
     assembly_gap    1062..1119
                     /estimated_length=58
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1120..1404)
                     /locus_tag="PLV68_11460"
     CDS             complement(<1120..1404)
                     /locus_tag="PLV68_11460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01908.1"
                     /translation="MVGTVGERGLGYQPALDGLRAIAVLAVVCFHGGLPWASGGYLGV
                     SVFFTLSGFLITSLLVAEIDDRGSVDLGRVGDVTECQVELLRSLPAAGQIP"
     gene            1446..2165
                     /locus_tag="PLV68_11465"
     CDS             1446..2165
                     /locus_tag="PLV68_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRE01909.1"
                     /translation="MSGPELYFRQLLSGRDFARNDPVARQMVNFAYAIGDRSTGETLL
                     VDPAYAVDDLVDAVEADGMRVVGALASHYHADHIGGSMMGYPIEGVTTLLERCQCPVH
                     LQRDETPWASKTTGISLDDVTQHSGGDVVTVGGIDVQLIHTPGHTPGSQCFLVQGRLV
                     SGDTLFLEGCGRTDLPGSNPEQMYESLQVLASLPDDTVIFPGHMYSQPDSASLGEVRR
                     YNYVFKPATKDAWMQWFGGAG"
     gene            complement(2182..2625)
                     /locus_tag="PLV68_11470"
     CDS             complement(2182..2625)
                     /locus_tag="PLV68_11470"
                     /inference="COORDINATES: protein motif:HMM:NF013224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HRE01910.1"
                     /translation="MTLDSIDKRIIAELQRDGRMSYADLAPMIGLSPAATRQRVQRLT
                     DSGVLQVVAVTDPLALGYHAMAMFCITVERDAESVADSVGALPAVIYSVLSAGGYDLF
                     AEVLCATNDELFEVLNDVRTIPGVREVRAFPYFRIHTHRFSWGVR"
     gene            2665..>3406
                     /gene="ligD"
                     /locus_tag="PLV68_11475"
     CDS             2665..>3406
                     /gene="ligD"
                     /locus_tag="PLV68_11475"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HRE01911.1"
                     /translation="MAADVIFAIDGREVVVSNPDKVFFSDRGDTKADLVRFYLTIGAP
                     LMHQMADRPVLLQRFPNGAGGSNFFQKRIPDSAPEWLTTTVVSTPNGTESRALVIADL
                     AHLIWAVNLGCLGFHSWPIRASDPAVTDELRLDLDPGPGTTFAMARETADEVRRLLTE
                     VGITGFVKTTGNRGLHVYVRLTPHWDSYAVRRAAVAVARELERRRPDLITAAWWKEER
                     GSRIFVDYNQNAPHKTVFAPWSVRARDHA"
     assembly_gap    3407..3517
                     /estimated_length=111
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3672..>3919
                     /locus_tag="PLV68_11480"
     CDS             3672..>3919
                     /locus_tag="PLV68_11480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01912.1"
                     /translation="MKLALAACLVAAVAYGVGAVMQALGARRAGLSTAGHDAHQAGTP
                     GQADAGRDLHEAGGIGTLLRQPIFLIGLLVDFAAWSISR"
BASE COUNT          641 a         1381 c         1211 g          517 t
ORIGIN      
        1 agtcgaccat ggccgacccc agcaccagtg ccgtcgagtc gccggcgacg atgacccgcg
       61 cgggcgccag cggctccggt tcgctcgaca gaacatcgac cggcgccgcg ctggatggcg
      121 atgcgttgtc gggcgcagtg ctgtccagcg gcacgcgctc cgatgtcgtg ctgggcggcg
      181 tcgtgccctg ctgcggcatc gagctgggcg gtggaacgat cgcgaccgcg gcggcgcgat
      241 cggcatcgac cgctccgatc ggaccggtcg ggccaggtac gaccgccacg gtggcgacga
      301 ccaacacggc gatgccgacc agcgcgccga tcagatcacg ccgtgcccgc acgccacctc
      361 ggcgcaccgg gcgttcgatc aaccagtacg acgccagcgt gatcgccgcc gtgaccacga
      421 cccgcaggac cgcgtcgtcg atcaaccgga acaccggcca gtggaacaga tagatgccgt
      481 agctgacctt gccgatcgcc accaacggac gccacgccag cgcccgacac aacgggccat
      541 cgaccatcgc cccggcgatc agcaccgaga cgatcacggc gaacaccggg aacccgcccg
      601 agtacggcca gccccgtccg ttcggggtca gcaccatcgc tcccaacacg acccccagcg
      661 cgcccactcc ggcgatgctc aacacacgtt gcgcctcgcg gcccacggcc cgccgttgac
      721 gccacatcac ggccagcacc gcaccgacca acaactcccc ggcgcgagcg ggcgtggaca
      781 ggtagaccac gttggcgccc caccgccgag cgatcagggg tgcggccgtc atcgccgtca
      841 tcgccaagac ccacaccaat gccaagcggg cggtcggctg gaactggtcg atcagacgcg
      901 gctccaccca gcgcgcccag ccccaccaca gcagcggcca caccagatag aactgctcct
      961 cgatcgccag cgaccaaaag tgggcaacgg gagaggtacc ggtgcggaac aggtcggcgt
     1021 acgactgctc cgctgtgagc tggccccagt tggcgacgtt gnnnnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc gggatctgtc cggctgcggg
     1141 caggctgcgc agcagctcga cctggcactc tgtcacatcg ccgacccgac cgagatcgac
     1201 cgagccacgg tcgtcgatct ccgcgaccag cagcgacgtg atcaggaagc ccgacagtgt
     1261 gaagaacacg gagacgccga gatagccgcc gctggcccac ggcaacccgc cgtggaagca
     1321 gaccaccgcc aacaccgcga tcgcccgcaa gccgtcgagg gcgggctggt agcccaaacc
     1381 gcgttcgccg accgtgccaa ccacgccggg agtgtacggg ccgggtcgcg cgtagggtga
     1441 acagcgtgag tggtcccgag ctctacttcc gccaactgct gtccgggcgc gacttcgccc
     1501 gcaacgaccc ggtcgcgcgc cagatggtca acttcgccta tgcgatcggt gaccgcagca
     1561 ccggcgagac cctgctggtc gacccggcct acgccgtcga cgacctggtc gacgcggtcg
     1621 aagccgacgg tatgcgcgtg gtcggagcgt tggcctcgca ttaccacgcc gaccacatcg
     1681 gcggctcgat gatgggctat cccatcgagg gggtgaccac cctgctcgag cggtgccagt
     1741 gccccgtgca cctccagcgc gacgagaccc cgtgggccag caagaccacc ggcatctcgc
     1801 tcgacgatgt gacccagcac agcggcggcg atgtggtcac cgtcggtggc atcgacgtgc
     1861 agctgatcca cacccccggc cacacgccgg gcagccagtg ctttctggtt caggggcgtc
     1921 tcgtctccgg cgacacgctg ttcctcgaag gctgcggccg caccgacctc cccggttcga
     1981 atcccgagca gatgtacgag agcctgcagg tgctcgcttc gttgccggat gacacggtga
     2041 tcttcccagg ccacatgtac tcccagcccg actcggcctc actcggcgag gtgcgccgct
     2101 acaactacgt gttcaagcca gcgaccaagg acgcctggat gcagtggttc ggcggcgccg
     2161 gctgacgagc gctggctctc gctaccgcac accccagctg aagcggtggg tgtggatgcg
     2221 gaagtacgga aacgcccgca cttcgcgcac acctggaatg gtgcgcacat cgttgagcac
     2281 ctcgaacagt tcgtcgttgg tggcgcacag cacctccgcg aacaggtcgt agccaccggc
     2341 ggacagaacg ctgtagatca ccgcgggcag tgccccgacg gagtccgcga cgctctcggc
     2401 gtcacgctcg accgtgatgc agaacatcgc catcgcgtgg tagccgaggg ccagcgggtc
     2461 ggtgaccgcg accacctgca acaccccgga gtcggtcaag cgctgcaccc gctggcgggt
     2521 cgcggccgga gagaggccga tcatcggcgc caagtcggcg tagctcatcc tgccgtcgcg
     2581 ttgcagttcg gcaatgatcc gcttgtcgat cgagtcgaga gtcacgggcc cgtaccctac
     2641 gaccgcttga gtagggtgcg ctgcatggcc gccgacgtca tcttcgccat cgacgggcgc
     2701 gaggtcgtgg tgagcaaccc ggacaaggtg ttcttttcgg accgtggcga caccaaggcc
     2761 gacctggttc gcttttacct gaccatcggc gcgccgctga tgcaccagat ggccgatcgc
     2821 ccggtgttgc tgcagcgctt tcccaacggt gcgggcgggt cgaacttctt ccagaagcgc
     2881 attcccgatt cggccccgga gtggctgacc accacggtcg tcagcacgcc caacggcaca
     2941 gagtcgcggg cgctggtcat cgccgacctc gcccatctga tctgggcggt caacctcggc
     3001 tgcctcgggt tccactcctg gccgatccgg gcgtcagacc ccgccgtcac cgacgagctg
     3061 cgcctcgatc tcgaccccgg gcccgggacc acgttcgcga tggcccgcga gacggccgac
     3121 gaggtgcgcc gcctgctgac cgaggtcggc atcaccggct tcgtcaagac caccggcaac
     3181 cgcggcctgc acgtgtacgt ccgcctcacc ccgcattggg actcgtacgc cgtccgccgt
     3241 gctgcggtcg cggtggcccg cgagctcgaa cggcgccgcc cggacctgat caccgcggcc
     3301 tggtggaaag aagagcgcgg cagccggatc ttcgtcgact acaaccaaaa cgccccgcac
     3361 aagaccgtgt tcgcaccgtg gtcggtgcgt gcccgcgatc acgcccnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntcg tctttctcaa ccagaccggc
     3541 actcccgatc ccgagcgcga cgagcgcaac gggctggcgg acgccccctg gccacccgtg
     3601 tacccgaaga tgcctggcga gcccgcacgg gtcgcgccga gcagggccaa gaagcccccg
     3661 gccgcagacg catgaagctc gcgctcgccg cctgcctggt ggccgcggtg gcatacggag
     3721 tcggagcagt gatgcaggcg ctcggcgccc gccgcgccgg gctcagcacc gccggccacg
     3781 acgcgcacca agcgggcacg ccaggccagg ccgacgctgg gcgcgacctg cacgaggcag
     3841 gcggcatcgg cacgctgctg cgccagccga tcttcctgat cggcctgctg gtcgacttcg
     3901 ccgcctggtc gatctcgcg
//
LOCUS       DAORNF010000276         3918 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_17330_length_3918_cov_1.688584, whole genome shotgun sequence.
ACCESSION   DAORNF010000276 DAORNF010000000
VERSION     DAORNF010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3918
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17330_length_3918_cov_1.688584"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>367
                     /locus_tag="PLV68_11485"
     CDS             <1..>367
                     /locus_tag="PLV68_11485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01913.1"
                     /translation="LRIIQVAMKTAASNAARTELLDRIDALQQELPLLVELELLREIV
                     SGRTVLPRWLRADLCRLLIHSEPTRRLGLAPGADAAALQESIETTGTGWRVLENTGRI
                     PFSARKAMLIAQQALDRLHA"
     assembly_gap    368..377
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            437..>872
                     /locus_tag="PLV68_11490"
     CDS             437..>872
                     /locus_tag="PLV68_11490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01914.1"
                     /translation="MPNDMITTPGSVLGERIETAPPAPMPGDLNDELIRTDIWTDDMV
                     FRAGIPVVDVPIVSCGGGMGSFVFTNYLRVAGLDTSKIRVLSNIDHPWATYEYLTKVS
                     QIPRPERLRSDSQSRPDNLWGFPGYALKEARQEKKLFPIWNVI"
     assembly_gap    873..981
                     /estimated_length=109
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <982..>1204
                     /locus_tag="PLV68_11495"
     CDS             <982..>1204
                     /locus_tag="PLV68_11495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01915.1"
                     /translation="LGQVRMVRRRNGGGYFTILTPPPGASQTRRVAFRSQYVHLAVGY
                     PGLKFLDDLQEYRTKYQDLARTVNAYEPHE"
     assembly_gap    1205..1223
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1224..2017
                     /locus_tag="PLV68_11500"
     CDS             <1224..2017
                     /locus_tag="PLV68_11500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01916.1"
                     /translation="GTVLVRGGGIVASRVLQRLMDDREKKGAQTQIIHLFRTYNSGPH
                     NKWGLAKRPGAHGFAFQGFNAPKSAWGGQHIQQMLKLEGPARKALYDLTGGAHTPLRK
                     YWLNQMKRGREQGWYSTYQGEVIEVRPGTNGVVTTIRQPNGSTAQLEAQIILDCTGLE
                     ADIREHRLLADLLDHSGAGRNPLGRLDVSPSFEVRGTQSGNGKLYASGSATYGGYFQG
                     VDTFLGLQLTAQRITDDLARQGFVPRIGMARSVSQWWKWFRHKKI"
     gene            2074..2535
                     /locus_tag="PLV68_11505"
     CDS             2074..2535
                     /locus_tag="PLV68_11505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01917.1"
                     /translation="MIPTLSGRIQTRIWLILFVGIPWTLIVTPFLPNLRDESGASLFD
                     TYKATFTVLGIVLVLAVFFWELVYHFLQQFRWEKDWPVMFTLLLVIPEGIVAFYVFKW
                     LDFNETYNFPATNGTFWWHLVSTWIVMWLFVLGPIRVLLPRWRFSGGRIFF"
     gene            2560..3381
                     /locus_tag="PLV68_11510"
     CDS             2560..3381
                     /locus_tag="PLV68_11510"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HRE01918.1"
                     /translation="MDHDTTEQPQSPQAIADYTDVRALDEGGHGRFFLAKAPPRLGVD
                     GDVVVKIVPGSSEAGFRRFTRELRLFARIDSPHLVRLYDAGQHEDSFFYSMEWCSRGT
                     LESPREPLDRAAKLKALAGIARAAHALHESGIAHRDIRPGNILLRVDGSACLGDLGLA
                     QLGSGSVTSMAPIASVGFVDPKLILGDTAGRASDIYSIGAVLHWALTGETLHPGLDTS
                     DPMMAVRSVLRGRVVVRRDLLSAAEADVVESCTADEITVRPPTAANVADLIDSLA"
     gene            3401..>3918
                     /locus_tag="PLV68_11515"
     CDS             3401..>3918
                     /locus_tag="PLV68_11515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01919.1"
                     /translation="MDFTLIPQLGAGRGRLIRTGATVLYSAGDAAAASALIGWCRVAA
                     TSSDVMGDLFRLAASANHPAARRRYHPARQRDRDDADQPAVSLRQRLADGATHLHEVS
                     TLELIIGDTAIDPLLSLNDGVVAADGFSLRAKATAALPWGPAPSAPDHAMAPVAESPA
                     AAAPPPQPAGPTA"
BASE COUNT          649 a         1335 c         1154 g          642 t
ORIGIN      
        1 gactgcggat catccaggtc gcgatgaaga ccgccgccag caatgcggcc cgcaccgagt
       61 tgctcgaccg catcgacgcg ctgcagcaag agctcccgct gctggtcgaa ctcgagctgt
      121 tgcgcgagat cgtctccggg cgcaccgtgc taccccgctg gctgcgggcc gacctgtgcc
      181 ggctgctgat ccactccgag ccgacccggc gtctcggtct ggcccccggt gccgacgcgg
      241 cggcgctgca agagtcgatc gagaccacgg gcacgggctg gcgtgtgttg gagaacaccg
      301 gacgtatccc gttcagcgcg cgcaaggcaa tgttgatcgc ccagcaagcc ctcgatcgcc
      361 tccacgcnnn nnnnnnntgg cctattacgg gtgacagacg cgcgatgatg taccccgtgc
      421 caagagagga atcaccgtgc cgaacgacat gatcaccacc ccaggcagcg tgctaggcga
      481 acggatcgag acggctcccc cagcgccgat gccgggagac ttgaacgacg agttgatccg
      541 taccgacatc tggaccgacg acatggtgtt ccgcgcgggc atccccgttg tcgacgtccc
      601 catcgtcagt tgtggtggcg ggatgggctc gttcgtgttc accaactatc tccgtgtggc
      661 ggggctggac acgtccaaga ttcgcgtcct cagcaacatc gaccatccat gggcgacgta
      721 cgaatacctc accaaggtca gccagatccc ccggcccgaa cgcctgcgca gcgacagcca
      781 aagtcgcccc gacaacctgt ggggctttcc tggctatgcg ctcaaagagg cccgacaaga
      841 aaagaagttg ttcccgatct ggaacgtgat cannnnnnnn nnnnnnnnnn nnnnnnnnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      961 nnnnnnnnnn nnnnnnnnnn nctgggccag gtgcgcatgg tgcgccgccg caacggcggc
     1021 ggctacttca cgatcctgac cccgccgccg ggggccagcc agacccgacg agtcgcgttc
     1081 cgcagccagt acgtccacct cgccgtcggc taccccggcc tcaagttcct cgacgatctg
     1141 caggaatacc gcaccaagta tcaagacctg gcgcgcacgg tcaacgccta cgagccgcac
     1201 gagcnnnnnn nnnnnnnnnn nnncgggcac ggtgctggtg cgcggcggcg gcatcgttgc
     1261 gtcgcgggtg ctgcagcgcc tgatggacga ccgcgagaag aagggcgcgc agacccagat
     1321 catccacctc ttccgcacct acaactccgg tcctcacaac aagtggggtc tggccaagcg
     1381 accaggagcc catggctttg cctttcaggg cttcaatgcg ccgaagagcg cctggggggg
     1441 tcagcacatc cagcagatgt tgaaactgga aggaccagcc cgcaaggcgt tgtacgacct
     1501 gaccggcggc gctcatacgc cgttgcgcaa gtactggctg aaccagatga agcgtggacg
     1561 cgagcagggt tggtactcca cctaccaggg cgaggtcatc gaggttcgtc ccggcaccaa
     1621 cggcgtcgtc accaccatcc gtcagccgaa cggatcgacc gcccagctcg aagcgcagat
     1681 catcctcgac tgcaccggct tggaagccga catccgcgag caccgcctgc tggccgacct
     1741 gctcgatcac tcgggtgccg ggcgtaaccc actcggccgc ctcgacgtgt caccctcgtt
     1801 cgaggtccga ggtacgcaga gcggcaacgg caagctctac gcgtcggggt cggccaccta
     1861 cggcggctac ttccaaggcg tcgacacctt cctcggcctc cagctcaccg cccagcggat
     1921 caccgacgac ctcgcccgtc agggcttcgt gccgcggatc ggcatggccc gctcggtcag
     1981 ccagtggtgg aagtggttcc gccacaagaa gatctgatct gacaacgatc tgaacagttc
     2041 gcccctcgca cctcgacccg gagacctctc gacatgatcc ccaccctgag tggacgcatc
     2101 caaacccgca tctggctgat cttgttcgtc ggcatcccgt ggacactgat cgtcacgccg
     2161 ttccttccca atcttcgcga cgagtccggc gcgtcgctgt tcgacaccta caaggcgacg
     2221 ttcaccgtgc tcggcatcgt gctcgtcctt gcggtgttct tctgggaact cgtctaccac
     2281 ttcctgcaac agttccgctg ggagaaggac tggccggtga tgttcaccct gttgctcgtg
     2341 atccccgaag gcatcgtggc gttctacgtg ttcaagtggc tcgacttcaa cgagacgtac
     2401 aacttccccg ctaccaacgg caccttctgg tggcacctgg tgtcgacctg gatcgtcatg
     2461 tggctgttcg tcctcggacc gatcagggtg ttgcttcccc gctggcggtt cagcggcgga
     2521 cgcatcttct tctgagcaca cccgcacgag agacgaacga tggatcacga caccactgag
     2581 cagccccagt cgccacaggc gatcgccgac tacaccgatg tgcgcgcgct cgacgaaggc
     2641 ggacatggca ggttctttct ggccaaggcg ccgccgcgac tcggcgtcga cggcgatgtc
     2701 gtggtcaaga tcgtgcctgg ctccagcgaa gccgggttcc gccgcttcac gcgtgagctg
     2761 cggctcttcg cgcgcatcga cagcccccac ctggtgcgct tgtacgatgc aggccagcac
     2821 gaggattcgt tcttctactc aatggaatgg tgttcgcgcg gcaccttgga atcgccgcgc
     2881 gaaccgctcg atcgtgccgc caagctcaag gcgttggcgg gcatcgcccg cgccgcccac
     2941 gccctgcacg agtccggcat cgcgcaccgc gacattcgcc ccggcaacat cctgctgcgc
     3001 gtcgacggct cggcctgcct cggtgacctc ggtctggccc aactcggctc gggttcggtc
     3061 acctcgatgg caccgatcgc atcggtcggc ttcgtcgacc cgaagctcat cctcggcgac
     3121 accgcgggtc gggcgtcgga catctactcg atcggtgcgg tgctgcactg ggcgctcacg
     3181 ggcgagacgc tgcaccccgg cctcgacacc agcgatccga tgatggccgt gcgctcggtg
     3241 ctgcgcggcc gcgtggtggt gcgccgcgat ctactcagcg cggcggaggc cgatgtcgtc
     3301 gagtcctgta ccgccgacga gatcaccgtc cgcccaccga cggccgccaa cgtggccgac
     3361 ctgatcgatt ccctcgcctg accaccggag aaccgccacc atggacttca cactcattcc
     3421 ccaactcggt gccggacgcg gccgcctgat ccgcaccggc gcgaccgtgc tctacagcgc
     3481 gggtgatgcc gccgcggcca gcgcgctgat cgggtggtgc cgggttgcgg caacctccag
     3541 cgatgtgatg ggcgatctgt tccggttggc cgcgagtgcc aaccatcctg ccgcacgtcg
     3601 tcgataccat cctgctcggc agcgcgatcg cgatgatgct gatcagccag cagtatccct
     3661 tcgccagcga ctggctgacg gcgccaccca tttgcacgag gtgagcacac tcgagctcat
     3721 catcggtgac acggcgatcg atcccctcct cagcttgaac gacggtgtgg tcgcggccga
     3781 cgggttcagc ctgcgggcca aggccaccgc tgcgctgccg tggggtccgg caccaagcgc
     3841 gcccgaccac gcgatggctc cagttgccga gtcaccggcc gctgctgcac cgccgccaca
     3901 gcccgccggg cccaccgc
//
LOCUS       DAORNF010000277         3918 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_17331_length_3918_cov_1.537665, whole genome shotgun sequence.
ACCESSION   DAORNF010000277 DAORNF010000000
VERSION     DAORNF010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3918
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17331_length_3918_cov_1.537665"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(134..1831)
                     /locus_tag="PLV68_11520"
     CDS             complement(134..1831)
                     /locus_tag="PLV68_11520"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HRE01920.1"
                     /translation="MSVAITEVSPWSSGDAPYGADWFELTNTGAEPVDLAGWKVDDSS
                     NDIASAALLSGVSTLAPGGSAVFFEGSDGDAPAIIAEFLTAWTGSAVAPVGFLIGNYS
                     GSGLGLSTGGDAVNVFDATGTNVASVTFGASSIGVTFDNTAGATGAISSLSVEGVNGA
                     FVAADGHGVGSPANLSVVPVDVLDYNPVGPVRVFDTRVGESPDALRSVAKRQVGGVVV
                     LEVQLTDLVGFVPARGVGAVSLNLTATGSSAAGFITVYACGTRELVSSVNFGAGETVA
                     NAVITPLSAAGTVCVFANTPVDVVADVNGWFAAGRAFEAVGPVRVFDTRAGESPEAVR
                     VVPVGRVVGGGVLEVQVTDLAGLVPAVGVGAVSLNVTVSNPAGSGFVTVYSCGAREFV
                     SSVNFVTGQTVPNAVIAPVSAAGTVCFFSTAATDLVVDINGWLQAGSGFTAVSPKRVF
                     DTRTGQSPDAMVDVVEQQVGGARVLEVDVSGLAGVTPAGGVGAVSINVTATGSTEPGF
                     VTVYACGTRELVSSVNFAAGQTVANAVLAPVSGAGTICFFSNTPVDIVADINGWFTR"
     gene            complement(1756..>2316)
                     /locus_tag="PLV68_11525"
     CDS             complement(1756..>2316)
                     /locus_tag="PLV68_11525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01921.1"
                     /translation="TTSVNLSGWRMDDSSASFASSVELIGVPTLPVGASAIFFEGTDE
                     DAFELAFAQAWFGQNLFDAGFFFGHYNGSGVGLSTGGDEVALFDAAGNLVTGVAFGNS
                     PSTAPYATFDNTSGLGSATKPFPVVTTLSAVGVNGAFAAVQVDEIGSPGTIGAVVIEP
                     DPCRWRSPRSHRGRPGTPPTARTGSS"
     assembly_gap    2317..2343
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2344..>2968)
                     /locus_tag="PLV68_11530"
     CDS             complement(<2344..>2968)
                     /locus_tag="PLV68_11530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HRE01922.1"
                     /translation="RLAVTEVAPWGSDAEYGADWFELTNTGGTTLELTGVRVDDSSNS
                     FANAVSLEGVASLAPGEAAIFLESSVVDRPATIERFAQAWFGDSGLPDGLQVGGYSGS
                     GIGLSGGGDEVNVFAVDGAHLTGISFAAATSNVTFDNAARLGAAAGAVPINTLAAEGI
                     NGAFLAADGVAVGSPGNVVPTTDPGSSGDTSGIRITEVAAFGSGNSPY"
     assembly_gap    2969..3177
                     /estimated_length=209
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3178..>3510)
                     /locus_tag="PLV68_11535"
     CDS             complement(<3178..>3510)
                     /locus_tag="PLV68_11535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01923.1"
                     /translation="TGAQTQAVKLGTTVGNIGLEGFSYDPLTTDGTGLGFIGVKESGP
                     LGLFQTTVDFATGTASNASPSTDNPANLFDPALVGTSDLSDVFPLSQLPDFEEGESPN
                     LVIISQESG"
     assembly_gap    3511..3712
                     /estimated_length=202
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3713..>3918)
                     /locus_tag="PLV68_11540"
     CDS             complement(<3713..>3918)
                     /locus_tag="PLV68_11540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01924.1"
                     /translation="SRFATVPFTPACPPPGGESSIDLSTYSLVGRYALPTHFDTPGAP
                     AGSELATEVSSITYNWDTDTLFVV"
BASE COUNT          679 a         1252 c         1071 g          478 t
ORIGIN      
        1 cgagaacggc atcggccagc cactgcgggg cgttgtcggg aaggatggct attcggggcg
       61 gggtggtact cgacatgatc aacccgaagc tagcagggtg ccgaaattcg gccgagggtc
      121 gggtcgattc ggatcagcga gtgaaccaac cgttgatgtc ggcgacgatg tcgactgggg
      181 tgttgctgaa gaagcagatc gtccccgccc cagacaccgg cgcgaggacc gcgttggcga
      241 cggtctgccc ggcagcgaag ttcacactcg acaccaactc ccgagtccca cacgcataca
      301 ccgtcacaaa cccgggctcc gtcgaaccgg ttgcggtgac attgatcgac accgcaccca
      361 caccacccgc aggcgtcaca cccgccagac cgctcacatc gacctcgagc actcgcgccc
      421 caccaacctg ctgctcgaca acgtccacca tcgcatcagg actctgcccc gtacgcgtat
      481 cgaacacacg cttgggactc accgccgtga acccgctccc ggcctgcaac cagccgttga
      541 tgtccacaac cagatccgtc gccgcagtcg agaagaagca caccgtgccc gccgccgaca
      601 ccggcgcgat caccgcattc ggcaccgtct gacccgtcac gaagttcaca ctcgacacga
      661 actcccgagc accgcaggaa tacaccgtca cgaacccact cccagcagga ttcgacaccg
      721 tcacattcaa cgacaccgca cccacaccca ccgccggaac caacccggcc agatccgtca
      781 cctgcacctc caagacccca ccaccaacaa cacgcccaac cggcacaacc cgcaccgctt
      841 caggactctc acccgcacga gtgtcgaaca cacgcaccgg acccaccgcc tcgaaggccc
      901 gcccagccgc gaaccaacca ttcacatcag cgacaacatc cacaggcgtg ttcgcaaaca
      961 cacacaccgt gcccgccgcc gacaacggcg tgatcaccgc gttcgctacc gtctcaccag
     1021 ccccaaagtt cacactcgac accaactcac gcgtcccaca cgcatacacc gtgatgaagc
     1081 ccgccgccga cgagcccgtc gccgtcaaat tcaacgacac cgcaccaacc ccacgagcag
     1141 gcacaaaccc aaccaaatcg gtcaactgca cctccaacac caccacacca ccaacctgac
     1201 gcttcgcaac cgaacgcaac gcatccggac tctcacccac acgcgtatcg aacacacgca
     1261 ccggacccac cgggttgtaa tccagcacat ccaccggcac caccgagagg ttcgccggcg
     1321 agcccacgcc gtggccatcg gcggccacga acgcaccgtt gacaccctcg acactgaggc
     1381 tgctgatcgc gcccgtcgcg ccggccgtgt tgtcgaaggt gacaccgatc gagctcgcac
     1441 cgaaggtcac cgacgccacg ttggttccgg tggcatcgaa gacgttcacc gcgtcgccgc
     1501 ccgtgctcaa cccgaggccg gagccgctgt aattcccgat caggaagccg actggcgcaa
     1561 cggccgagcc cgtccacgcc gtgaggaatt cggcgatgat cgccggagcg tcgccgtcgg
     1621 acccctcgaa gaagacggcc gagccgccgg gcgcaagggt gctcacgccg ctgagcagcg
     1681 cggcgctggc gatgtcgttc gagctgtcgt cgaccttcca cccggcgagg tcgacaggct
     1741 cggcgccggt gttggtcagc tcgaaccagt ccgcgccgta gggggcgtcc ccggacgacc
     1801 acggtgagac ctcggtgatc gccaccgaca cgggtccggc tcgatgacga cagctccgat
     1861 cgtccctggc gagccgatct cgtcgacctg cacggcggcg aacgcgccgt tgaccccgac
     1921 ggcgctcaac gtcgtcacca ccgggaaggg cttggtggcc gaaccgagcc cggaggtgtt
     1981 gtcgaaggtg gcgtagggcg cggtcgacgg cgagttgccg aaagcgacac cggtgaccag
     2041 gttccccgcg gcgtcgaaga gagccacctc gtcgcccccg gtcgacagac ccacgccact
     2101 gccgttgtag tggccgaaga agaaccctgc gtcgaacagg ttctgcccga accacgcctg
     2161 ggcgaacgcg agctcgaagg cgtcctcgtc ggtgccctcg aagaagatcg ccgaggcccc
     2221 gacgggcagc gtcggcacgc cgatcaattc gacgctgctc gcgaacgagg ccgacgagtc
     2281 gtccatccgc catccgctca agttgaccga ggtggtnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnncatacgg cgagttgccc gatccgaatg cggcgacctc ggtgatccgg atgccggacg
     2401 tgtcgccgga cgaaccagga tcggtggtgg gaacgacgtt gccaggggaa ccgacggcca
     2461 cgccgtcggc cgccaggaag gcaccgttga tgccctccgc ggccagcgtg ttgatcggca
     2521 ccgcgcctgc ggcggcgcca agacgggccg cattgtcgaa ggtgacgttg ctcgtcgcgg
     2581 cggcgaagga gatcccggtg aggtgcgcgc catcgacggc gaagacgttc acctcgtcac
     2641 cgccaccgga caggccgatt cccgagcccg agtacccgcc gacctgcagg ccgtccggca
     2701 ggccggagtc gccgaaccag gcctgggcga accgttcgat cgtcgccggg cgatcgacaa
     2761 cgctcgattc gaggaagatc gcggcctcgc ccggcgcgag cgaggcgacg ccttcgagcg
     2821 agaccgcgtt cgcgaacgag ttggagctgt cgtcgactcg cactcccgtg agctccagtg
     2881 tggtcccgcc ggtgttggtc aactcgaacc agtccgcgcc gtactccgcg tcgctgcccc
     2941 acggggcgac ttcggtgacg gcgagtcgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnccc
     3181 gactcctggc tgatgatcac gaggttgggt gactcgccct cctcgaagtc cggcaactgc
     3241 gacagaggga agacgtcgga gagatcggag gtgccgacga gcgcgggatc gaacaggttc
     3301 gcggggttgt ccgtcgacgg cgaggcgttg ctcgccgtcc cggtcgcgaa gtcgaccgtg
     3361 gtctggaaca acccgagcgg cccgctctct ttgacaccga tgaacccgag cccggtgcca
     3421 tcggtggtga gcgggtcgta gctgaaaccc tccaggccga tgttgccaac ggtggtgccc
     3481 agcttcactg cttgggtttg cgcgccggtg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngacgacga
     3721 agagcgtgtc ggtgtcccag ttgtacgtga tcgacgacac ctcggtggcc aactcggatc
     3781 ctgccggggc gccgggggtg tcgaagtggg tcggcagggc gtagcgcccg acgagtgagt
     3841 aggtcgagag atcgatgctc gactcgccgc cgggcggagg gcaggcgggc gtgaagggca
     3901 ccgtggcgaa gcggctgg
//
LOCUS       DAORNF010000278         3916 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_17346_length_3916_cov_1.514893, whole genome shotgun sequence.
ACCESSION   DAORNF010000278 DAORNF010000000
VERSION     DAORNF010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3916
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17346_length_3916_cov_1.514893"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>374)
                     /locus_tag="PLV68_11545"
     CDS             complement(<1..>374)
                     /locus_tag="PLV68_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HRE01925.1"
                     /translation="GGCSVADLAAEYGTPLFIYDEAHLRARCREAVAAFGHQNAVYAT
                     KAFLCKAMARLAYDEGMLLDVATGGELHVALSAGVPASACTMHGNNKSIGELRTAITA
                     GVGQIVVDSFDEMDRLDALARG"
     assembly_gap    375..469
                     /estimated_length=95
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(557..>1584)
                     /gene="argS"
                     /locus_tag="PLV68_11550"
     CDS             complement(557..>1584)
                     /gene="argS"
                     /locus_tag="PLV68_11550"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HRE01926.1"
                     /translation="FNSAYRTLGVLLTDDDLAGESGYDPLLPAVIERLRDAGLLTQSD
                     GADVVFPPGYTNRDGEPLPLIVQKRGGGFNYATSDLACVIDRVERVQADLMLYVVGAP
                     QAQHLAMVFAVAEMAGWLVPPTAAVHVAFGNVLGPDKKMLKSRSGDAMKLIELLDEAE
                     TRAAAAVADKNPDLPAAERAAIAHAVGIGAVKYADLSTDRIKDYIFDWERMLSFDGNT
                     APYLQYAHVRICSIFRRAEIERDAFRVTHPQVTLTAPQERALALRLLAFDSAVWDAID
                     KYAPHKLCTYLFDLAQDFTAFFEHCPVLRAPDEATTTSRLALCDLTARIMSRGLDLLG
                     IDAPERM"
     assembly_gap    1585..1797
                     /estimated_length=213
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1798..>2040)
                     /gene="argS"
                     /locus_tag="PLV68_11555"
     CDS             complement(<1798..>2040)
                     /gene="argS"
                     /locus_tag="PLV68_11555"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HRE01927.1"
                     /translation="NLTFADSFLATELAAIAADDHLGVHSQPNPKRVVVDYSAPNVAK
                     EMHVGHLRSTVIGDSLVRLLTFVGDTVIRENHIGDW"
     assembly_gap    2041..2206
                     /estimated_length=166
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2326..2400
                     /locus_tag="PLV68_11560"
     tRNA            2326..2400
                     /locus_tag="PLV68_11560"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2359..2361,aa:Arg,seq:ccg)
     assembly_gap    2518..2605
                     /estimated_length=88
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2606..>3411)
                     /locus_tag="PLV68_11565"
     CDS             complement(<2606..>3411)
                     /locus_tag="PLV68_11565"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRE01928.1"
                     /translation="TAVVLPTSPRRSRAKVDVVGAVLLSAAVTCVVLFTTWGGTQYAW
                     GSGTMIGLAVAAVVLLVALVVVERRVDEPIVPMHLFGLRTFKIASVVSFIAGVAMFGA
                     ISFLPLFLQVVNQASATNSGLLLLPLMLSILAESLVAGQVTSRTGHYKPFPIIGCGLA
                     TIAMYLLSTMHAGTAQSSVTVYMAILGAGIGFTLGTLILAVQNAVELHDLGSATSTVS
                     FFRSTGGAIGVAMFGALFNNLLASRVGATIDIAEGSSFTPSSVAALPEAE"
     assembly_gap    3412..3423
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3424..>3916)
                     /locus_tag="PLV68_11570"
     CDS             complement(<3424..>3916)
                     /locus_tag="PLV68_11570"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRE01929.1"
                     /translation="IFSGLMLGMLLAALDQTIVTTALPTITGELGGLNHLSWVVTSYL
                     LASTVATPLYGKLGDLFGRKRLFQISIVIFLVGSALCGVAQSMMQLIAFRAVQGLGGG
                     GLMVLAQAIIAEVVSPRERGRYQGYFGGVFAMASVGGPLLGGFLTDQISWRWVFYVNL
                     PFGV"
BASE COUNT          599 a         1118 c         1121 g          504 t
ORIGIN      
        1 cgccgcgcgc cagcgcgtcg aggcggtcca tctcgtcgaa gctgtcgacg acgatctggc
       61 cgacaccagc ggtgatcgcg gtgcgcagtt ccccgatgct cttgttgttg ccgtgcatcg
      121 tgcaggcgga cgcgggcacc ccggcgctga gggcgacgtg cagttcgccg ccggtggcga
      181 cgtcgagcag catcccctcg tcgtaggcca agcgggccat cgccttgcac aggaaggcct
      241 tggtggcgta cacggcgttc tggtggccga acgcggcgac ggcctcacgg caccgggcgc
      301 gcaggtgagc ttcgtcgtag atgaacagcg gcgtgccgta ctcggcggcc aggtcggcca
      361 ccgagcaccc gccgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt gcgcccccgc
      481 tccgctgcgc tccgcgcccc atcgacgccg ggctcgctgg cgctcgcacc gtcgttgttc
      541 gctccgctca ctgcgctcac atgcgctccg gggcgtcgat gccgagcagg tcgaggccgc
      601 gggacatgat ccgcgcggtc agatcgcaca gggcaaggcg gctggtcgtg gtggcctcgt
      661 cgggagcgcg cagcacgggg cagtgctcga agaacgcggt gaagtcctgc gccagatcga
      721 acaggtaggt gcacagcttg tgcggcgcgt acttgtcgat ggcatcccac accgcgctgt
      781 cgaaggccaa caggcgcaag gccagcgccc gctcttgcgg tgcggtgagg gtgacctgcg
      841 gatgcgtgac ccggaacgca tcccgctcga tctcggcccg acggaagatg gagcagatgc
      901 gcacgtgggc gtactgcagg tacggcgcgg tgttgccgtc gaaggacagc atccgctccc
      961 agtcgaagat gtagtccttg atgcggtcgg tcgacaggtc tgcgtacttg acggcaccga
     1021 tgcccaccgc atgagcgatg gcggcccgtt ccgcggcggg caggtcgggg ttcttgtcgg
     1081 cgaccgccgc ggcagcccgc gtctcggcct cgtcgagcaa ctcgatcagc ttcatcgcat
     1141 cgcccgaccg gctcttcagc atcttcttgt cggggccgag cacgttgccg aacgcgacgt
     1201 ggaccgctgc ggtcggcggc acgagccaac cggccatctc cgcgacggcg aacaccatcg
     1261 ccagatgctg ggcctgcggc gcgccgacga catagagcat caggtcggcc tgcacccgct
     1321 cgacccgatc gatcacgcac gccagatcgc tggtggcgta gttgaacccg cccccccgct
     1381 tctgcacgat cagtggcagt ggctcgccgt cacggttggt gtagccgggt gggaacacca
     1441 catcggcacc gtcactctgg gtgagcagtc cggcgtcgcg tagccgttcg atcacagcgg
     1501 gcagcaacgg gtcgtagccg ctctcgcccg cgaggtcgtc gtcggtcagc agcaccccca
     1561 gcgtgcgata ggcgctgttg aagtnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnccc
     1801 agtcaccgat gtggttctcg cggatcaccg tgtcgccgac gaaggtcaag agccgtacga
     1861 gcgagtcacc gatcaccgtg ctgcgcaggt ggccgacgtg catctccttg gccacgttgg
     1921 gcgcggagta gtcgaccacg acccgcttgg ggttgggttg ggagtgcacg ccgaggtggt
     1981 cgtccgccgc gatcgcggcg agttcggtcg ccaggaacga gtcggcgaag gtgaggttga
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncgat ggcagcgaag
     2221 acgggctcga gcaagcggga tacggaaacg acgggatcag ccatggactc cccattctgc
     2281 ccgcccaaag ccccgctcac cccgctagat tgcgagggct tccccgccct cgtagctcag
     2341 gggatagagc atcggtttcc ggaaccgtgt gcgggagttc gaatctctcc gagggcgcca
     2401 gcgcatctcc cccggttcgt cggattcgcg gccgcgccgg gtcagtgcat cgccgcggcg
     2461 gctgaacctg acgaggtcgt ggtgtggacg gacaggcgga gggggacttc gtgcagcnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnngctcg gcctcgggga gcgcggcaac agaggacggc
     2641 gtgaagctgg agccttcggc gatgtcgatc gtggcaccga cccggctggc gaggaggttg
     2701 ttgaacagcg cgccgaacat ggcgacgccg atcgcaccgc ccgtcgaccg gaagaagctc
     2761 accgtcgatg tcgccgagcc gagatcatgg agctcgactg cgttctgcac cgccagaatc
     2821 aacgtgccga gggtgaagcc gatgccggca cccaggatgg ccatgtacac ggtgacactc
     2881 gactgagcag tgccagcatg catcgtcgac agcagataca tcgcaatcgt cgccaacccg
     2941 catccgatga tcgggaacgg tttgtagtgt ccggtacggc tggtgacctg gcctgcgacc
     3001 aatgattccg ccaagatgct cagcatcagg ggcagcagca acaagccgga gttggtggcc
     3061 gacgcttggt tgaccacttg gagaaacaac ggcaagaagc tgatcgcacc gaacatcgcg
     3121 acgccggcga tgaagctcac gacggacgcg atcttgaagg tgcgcagtcc gaacaggtgc
     3181 atcggcacga tcggctcgtc gacccgccgc tcgaccacga ccaacgccac cagcaagacc
     3241 accgcggcaa cggcgagtcc gatcatggtg cccgatcccc acgcgtactg ggttccgccc
     3301 caggtggtga acagcaccac gcaggtcacc gccgccgaca gcaacaccgc acccaccaca
     3361 tcgaccttgg cccggctgcg tcgcggtgac gtcggcagca ccaccgcggt gnnnnnnnnn
     3421 nnncgacacc gaagggcagg ttcacgtaga acacccaccg ccacgagatc tggtcggtca
     3481 ggaaaccgcc gagcaacggg ccacccacgc tcgccatcgc gaagacccca ccgaagtagc
     3541 cctgataccg cccgcgctca cgtggcgaga cgacctcggc gatgatcgcc tgggcgagca
     3601 ccatcaaacc gccgccaccg aggccctgga cggcccggaa ggcgatgagc tgcatcatcg
     3661 actgcgcaac tccgcacagc gcgctgccga ccaggaagat cacgatcgag atctggaaca
     3721 gccgcttgcg gccgaacaga tcgcccaact tgccgtacag cggcgtcgca accgtggacg
     3781 ccagcagata gctcgtcacc acccatgaca ggtggttgag cccgcccaac tcgccggtga
     3841 tcgtgggtag cgcagtggtg acgatggtct ggtcgagcgc ggccagcagc atgcccaaca
     3901 tcaagccgct gaagat
//
LOCUS       DAORNF010000279         3914 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_17361_length_3914_cov_1.803058, whole genome shotgun sequence.
ACCESSION   DAORNF010000279 DAORNF010000000
VERSION     DAORNF010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3914
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17361_length_3914_cov_1.803058"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1176)
                     /locus_tag="PLV68_11575"
     CDS             complement(<1..1176)
                     /locus_tag="PLV68_11575"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HRE01930.1"
                     /translation="MSTPSVAARPANLANFIWQIADQLRGVYKPHQYGNVILPMTILR
                     RLDAVMAPHRATMRTLAAATDNADVLARQVKKQTGLGFSCTSPYDFALLLSDPAGLGD
                     NLLDYINRFSPNVDVFTRFKFDNEVATLAEKDRLYLVVKQFADVDLHPDTVPNAQMGD
                     LFEELIRKFAEASNEEAGEHYTPRDAIRLMVDLLFAEDNEALTRPGVIRSVYDPTAGT
                     GGMLSLAEEHLLQQNPSAVMVLYGQEINDQSYAICKSDMIVKGQDATNIQMGDTLAND
                     RFAGKTFDYCLSNPPYGVDWKASEQAIKAERAATGANGRFGAGLPKISDGQMLFLSHL
                     AHKMRPERDGGGRAAIVLNGSPLFNGAAESGESEIRRWLLESDLVDAIVALPTSMFYN
                     "
     gene            1305..>2555
                     /locus_tag="PLV68_11580"
     CDS             1305..>2555
                     /locus_tag="PLV68_11580"
                     /inference="COORDINATES: protein motif:HMM:NF013964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HRE01931.1"
                     /translation="MSATTSLSGEELDALWADVVTQAGVLNAAHARLVALTEKALRHG
                     AWGGEGVRSPTHWLCWQTGVSRSTADKILQLASSRDTHPEVMASFDAGALSLDQASVA
                     VQAPPHCDHDIATLAVLSTVSQIRTEVRVANQTRPNDVPAAADQSAASAQPETSTDAT
                     SPDDVAADGDDAFDPSQPMVVPVVDEAPSSVRLLWGTDGRLSIRGDLTVDDGTLLEAA
                     LLAAKDRLFHSGQEATLARALLDIAQRGLDAEPAERRERFRINLFLAPDASQAQWTNH
                     VPVPDWLLSQLCCTTSVSPVHTDAAGLPVSVGRSAKALPDRTRRLVLHRDRGRCQNPR
                     CGSRVGLHIHHIWFWSRGGPTDTNNLVTVCGRCHRAVHTGHLRVHGNPDDPGGATFTE
                     RSGRPIERPIAGPPAEPPPQPDPPY"
     assembly_gap    2556..2621
                     /estimated_length=66
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2664..>3105)
                     /locus_tag="PLV68_11585"
     CDS             complement(2664..>3105)
                     /locus_tag="PLV68_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HRE01932.1"
                     /translation="GGLIVAAGCDLRVAARSARFSIPEVDLGIPLAWGGIPRLVREIG
                     PALTRELVLTCRPFGPAEARAIGFLNRVVPRAELESTVDELAVSLARKAPSVVRATKR
                     QVNEALDAMATTAGSWADADQLGAALRDPEARAAARTYLGARAK"
     assembly_gap    3106..3276
                     /estimated_length=171
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3277..3579)
                     /locus_tag="PLV68_11590"
     CDS             complement(<3277..3579)
                     /locus_tag="PLV68_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01933.1"
                     /translation="MAYLIGRRLRDASPRPDVYESARFGGAREHATMTEVPQFDTLSV
                     ALDERTGDDESADGRIGRLTLTQGDKLNPLGTVPLREIAAAARWFDTTSASVVIVTG"
     gene            3566..3865
                     /locus_tag="PLV68_11595"
     CDS             3566..3865
                     /locus_tag="PLV68_11595"
                     /inference="COORDINATES: protein motif:HMM:NF016843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPP family protein"
                     /protein_id="HRE01934.1"
                     /translation="MRYAKAEIARGALGALVGIAVAGASARLVPGGPELLPFIIAPMG
                     AVAVLLFAVPASPLAQPWPALGGNLISTAIGLAAHFLIDDVLTAAAVGNPVWAWA"
BASE COUNT          553 a         1288 c         1254 g          582 t
ORIGIN      
        1 gttgtagaac atgctcgtcg gcagggccac gatcgcatcg accaggtcgc tctccagcaa
       61 ccagcggcgg atctccgact cgccgctctc cgcggcgccg ttgaacaacg gcgacccgtt
      121 gaggacgatc gccgcccgcc cgccgccgtc acgttcgggc cgcatcttgt gggccaggtg
      181 cgacaggaac agcatctggc cgtcgctgat cttgggcagc ccggctccga agcgaccgtt
      241 cgcacccgtc gcggcccgct cggccttgat cgcctgctcc gacgccttcc agtccacgcc
      301 gtagggcggg ttcgacaggc agtagtcgaa ggtcttgccc gcgaagcggt cgttggccag
      361 ggtgtcgccc atctggatgt tggtggcgtc ctggcccttg acgatcatgt cgctcttgca
      421 gatcgcgtac gactggtcgt tgatctcctg cccgtacagc accatcaccg ctgacgggtt
      481 ctgctgcagc aggtgttcct cggccaggct caacatgccg ccggtgcccg ccgtcgggtc
      541 gtacacggaa cggatcaccc cgggccgggt cagcgcttcg ttgtcctccg cgaacagcag
      601 gtccaccatc aggcggatcg cgtcacgcgg ggtgtagtgc tcgccggctt cctcgttgga
      661 cgcctcggcg aacttgcgga tcagttcctc gaacaggtcg cccatctgcg cgttggggac
      721 ggtgtcgggg tgcaggtcga cgtcggcgaa ctgcttcacc accaggtaca gccggtcctt
      781 ctcggccagg gtggcgacct cgttgtcgaa cttgaaccgg gtgaacacgt cgacgttcgg
      841 cgagaagcgg ttgatgtagt cgagcaggtt gtcgcccaga cccgcggggt cgctgagcag
      901 caaggcgaag tcgtacggcg aggtacacga gaagccgaga ccggtctgct tcttcacctg
      961 gcgggcgagc acgtcggcgt tgtcggtcgc cgcggcgagc gtgcgcatcg tcgcgcggtg
     1021 cggggccatc accgcgtcga gccggcgcag gatcgtcatc ggcaagatca cgttgccgta
     1081 ctggtgcggc ttgtacacgc cgcgcaactg gtcggcgatc tgccagatga agttggccag
     1141 gttggcaggg cgcgcggcca cagacggtgt cgacacgatg aagacggtag acgttcgcag
     1201 cgatgaggaa cggcacggtc acacgtcatg tcatgccgca gtgggggcca tcgatcgccg
     1261 ccgggcggga aaccctgtga cacccctcgc gtatggtgcc gagcatgtcg gcgaccacct
     1321 ctctcagcgg cgaggagctg gatgcgttgt gggcggacgt cgtcacccaa gcaggtgtgt
     1381 tgaacgctgc ccatgcacgt ctcgtcgcgt tgactgagaa ggcgttgcgg catggggcgt
     1441 ggggcggcga aggggtccgt tcaccgacgc attggttgtg ctggcagacc ggggtatcgc
     1501 gttccaccgc cgacaagatc ctgcagctgg ccagctcccg cgacacccac cccgaagtga
     1561 tggcctcctt cgacgctggc gcgctgtcgc tcgaccaggc gtcggtcgcg gtgcaagcgc
     1621 ctccgcactg cgaccacgac atcgccacac ttgccgtgct ctccaccgtg agtcagatcc
     1681 gcaccgaagt ccgtgtcgcc aaccagaccc gcccgaacga tgtgcccgct gctgcagatc
     1741 agtccgctgc atcggcacag ccggagacgt ccaccgatgc caccagccca gacgatgtgg
     1801 cagccgacgg cgacgacgcc ttcgacccgt cgcagcccat ggtggtgccg gtggtcgacg
     1861 aggcgccgtc cagcgtgcgg ctgctctggg gcaccgatgg acggctctcg attcggggtg
     1921 atctcaccgt tgacgacggc acgctgctcg aagcggcact cttggcagca aaggaccggc
     1981 tctttcacag tggacaagaa gccacactcg cccgcgccct gctcgacatc gcccagcgtg
     2041 ggctcgatgc cgaaccggcc gagcgacgcg aacgattccg catcaacctc ttcctggcac
     2101 ccgacgccag ccaggcgcag tggaccaacc atgtgccggt gcctgactgg ctgctgagcc
     2161 agctgtgctg caccacctcg gtcagcccgg tgcacaccga cgctgccggc ctgccggtat
     2221 cggtcgggcg ctccgccaag gcactccccg accgcacccg tcgcctcgtg ctgcaccgcg
     2281 accgtggccg gtgccagaac ccccgatgcg gttcacgcgt cgggctgcac atccaccaca
     2341 tctggttctg gtcgcgtggc ggtcccaccg acaccaacaa cctcgtcacg gtgtgcggtc
     2401 gttgccaccg agccgttcac accgggcacc tccgcgtgca cggcaacccg gacgaccccg
     2461 gcggcgccac cttcaccgag cgatctggcc gacccatcga acgcccgatc gccgggccgc
     2521 ccgccgaacc gccaccacag cccgacccgc cgtacnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nccggcccgc gccccgaacc
     2641 cgaacaccgc cgacgcggcg tagctacttg gcgcgagcgc cgaggtaggt ccgtgcggcg
     2701 gcgcgggctt cggggtcgcg cagggcagcg ccgagttggt cggcgtcggc ccagcttccc
     2761 gcggtggtcg ccatggcgtc gagcgcctcg ttcacctggc gcttcgttgc ccgaaccacg
     2821 ctcggtgcct tgcgggcgag cgacacggcg agttcgtcga ccgtcgattc caactcggcg
     2881 cggggcacca cccggttcag gaagccgatc gcgcgcgcct cggcggggcc gaatgggcga
     2941 cacgtcagca ccagctcgcg cgtcagcgcc gggccgatct cgcggaccag gcgggggatg
     3001 ccaccccacg cgagcgggat gccgaggtcg acctcgggga tcgagaagcg tgccgagcgg
     3061 gcagccacgc gcaggtcgca tcccgcggcg acgatcagtc caccgnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngccg gtgacgatca cgaccgacgc
     3301 cgaggtggtg tcgaaccagc gggctgcggc ggcgatctcg cgcagtggca ccgtgccgag
     3361 cgggttcagc ttgtcgccct gcgtcagcgt caaccggccg atccggccgt cagcgctctc
     3421 gtcgtcgccg gtccgctcgt ccagggcgac ggacagcgtg tcgaactggg gtacctcggt
     3481 catggtcgca tgctcgcgcg cgccgccgaa gcgagccgac tcgtacacgt ccgggcgcgg
     3541 cgaggcgtcg cgtagtctgc ggccgatgag atacgccaag gcggagattg cccggggggc
     3601 gttgggggcc ttggtgggca tcgccgtagc gggggccagc gctcgattgg tgccgggtgg
     3661 gccggagttg ttgccgttca tcatcgcccc gatgggcgcc gtggcggtgt tgctgttcgc
     3721 cgtgcccgcc agcccgttgg cccagccgtg gccagcgctg ggcggcaacc tgatctcgac
     3781 cgcgatcggg ctcgccgcgc acttcctgat cgacgatgtg ctcaccgcgg ctgcggtcgg
     3841 aaaccccgtg tgggcgtggg cctgacaaac ccgcgcacca acgcgaacgg cgcccgacct
     3901 cgaagcgagg cggg
//
LOCUS       DAORNF010000280         3912 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_17371_length_3912_cov_1.730360, whole genome shotgun sequence.
ACCESSION   DAORNF010000280 DAORNF010000000
VERSION     DAORNF010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3912
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17371_length_3912_cov_1.730360"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>547
                     /locus_tag="PLV68_11600"
     CDS             <1..>547
                     /locus_tag="PLV68_11600"
                     /inference="COORDINATES: protein motif:HMM:NF016843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPP family protein"
                     /protein_id="HRE01935.1"
                     /translation="AIGAMMLLRCLHPPGGACALLAATATPAIHQQGALFVLSPVAIN
                     VVALLVIAYLVHNLTGHHYPHRPAKPQPVGPAQAALGITTPDVEEAMKRLADRLDVLP
                     ADVVAIVQDAENHALDRRLGSIPVARIMNTDPAVVHPFESIYRARLLFTQRGVKTLPV
                     IDNERHVVGVISIIDVFTRELV"
     assembly_gap    548..686
                     /estimated_length=139
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(753..>1003)
                     /locus_tag="PLV68_11605"
     CDS             complement(753..>1003)
                     /locus_tag="PLV68_11605"
                     /inference="COORDINATES: protein motif:HMM:NF013575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01936.1"
                     /translation="WSDVSSEFTAFTDQRVLRFEIRANAFCQQMVRSIVGMMIDVGTG
                     VRHAGEVRGIMRARDRRGAPRLAPAHGLCLWDVGYPAD"
     assembly_gap    1004..1030
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1031..1595)
                     /locus_tag="PLV68_11610"
     CDS             complement(<1031..1595)
                     /locus_tag="PLV68_11610"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine synthase A"
                     /protein_id="HRE01937.1"
                     /translation="MPLDGIDTALNEPVALRRVRLVVAYDGRGFRGFAINHQVRTVMG
                     VLTDALSTITRHPVELAGAGRTDAGVHGWGQVVSGDIPADADLTGVVRRANAMCGPEV
                     VVRSAEWAGDTFHARFSALWRHYRYTVLNAAQPNPFLEATAWRMHRPLSLPAMQLACD
                     PLIGEQDFTSFCRVPDRDPDQPPPSMHR"
     gene            complement(1600..1971)
                     /gene="rplQ"
                     /locus_tag="PLV68_11615"
     CDS             complement(1600..1971)
                     /gene="rplQ"
                     /locus_tag="PLV68_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HRE01938.1"
                     /translation="MPATPRRAPNFGGSSQHQKLMMANLAASLIAAEAIVTTEAKAKA
                     LRPIAEKLITKAKKAGDADPMRVHRIRQIQGYLGDREMTHKLVNDVAPRYTERNGGYL
                     RILKLGPRQGDNAPMARIELV"
     gene            complement(1998..2930)
                     /locus_tag="PLV68_11620"
     CDS             complement(1998..2930)
                     /locus_tag="PLV68_11620"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HRE01939.1"
                     /translation="MLVIQRPTVEAIGEESNNRQRFAIGPLEPGFGHTIGNSLRRTLL
                     SSIPGAAVTAVRFDDALHEFDTIQGVAEDITDVILNIKDIVLTSTSDEQVIIRLDVRG
                     PAEVSAADIKCPSEVEILNGDLHLASINAKGRLAIDLTVERGRGYLSSNRDSDSRVIG
                     VIPVDAIFSPVRRVTFEVEPTRVEQSTNFDRLVLDIETDGSVAPRDALASAGATLRSL
                     VDLVANLSEEPQGLELGEVAATSATSPDLDLPIEDLDLSERPRNCLKRAQINSIGELL
                     TKNEDDLLNITNFGQKSLDEVKQKLDERGLTLRG"
     gene            complement(2970..3587)
                     /gene="rpsD"
                     /locus_tag="PLV68_11625"
     CDS             complement(2970..3587)
                     /gene="rpsD"
                     /locus_tag="PLV68_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HRE01940.1"
                     /translation="MARYTGPKVRVSRRLSTNIFENEKGRKALERRPFPPGQHGRTRR
                     RNAGSEYLAQLQEKQKAKYIYGVLERQFRKTYDEANRLQGPTGENLLRLLEQRIDNIV
                     YRAGWAETRPQARQLVSHGKILVNGKRLDIPSARVKRGDVVSLSEKARNMIVVQHNLD
                     VLDRRVVGWLEVGDGGKQVTVRDLPQREHIDVPVREQLIVELYSK"
     gene            complement(3591..>3912)
                     /gene="rpsK"
                     /locus_tag="PLV68_11630"
     CDS             complement(3591..>3912)
                     /gene="rpsK"
                     /locus_tag="PLV68_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016998822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HRE01941.1"
                     /translation="KSSFNNTIVSVTDTEGNVISWASSGGVGFKGSRKSTPFAAQMAA
                     DKAARAAMEHGLRRVEVQVKGPGSGRDTAVRSIQNTGLEVTSIKDVTPLAHNGCSQPK
                     RKRS"
BASE COUNT          561 a         1222 c         1308 g          655 t
ORIGIN      
        1 gcgatcgggg cgatgatgct gctccgctgc ctgcacccac cgggtggcgc gtgcgcgctg
       61 ctggccgcca ccgcgacacc agcgatccac cagcaggggg cactgttcgt gctctcgccc
      121 gtcgcgatca acgtcgtcgc actactggtc atcgcctatc tcgtccacaa cctgaccggc
      181 caccactacc cgcaccgccc ggcgaagccg caaccggtcg gccccgccca agccgcgctc
      241 ggcatcacca cccccgacgt cgaggaagcg atgaagcgcc tggccgaccg gctcgacgtg
      301 ctgcccgccg acgtcgtcgc catcgtccaa gacgccgaga accacgccct cgaccggcgg
      361 ctggggtcga tcccggtggc gcggatcatg aacaccgacc cggccgtggt ccatccgttc
      421 gagtcgatct atcgcgcccg gctgctgttc acccaacgcg gcgtcaagac cctgccggtg
      481 atcgacaacg agcgccacgt cgtcggcgtg atctcgatca tcgacgtgtt cacccgcgag
      541 ttggtcgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      661 nnnnnnnnnn nnnnnnnnnn nnnnnntgat cactcgcggc gagttgatcg cggtgctcca
      721 ccgcgccctg ctcggcgcca cctgacctgc gctcagtcgg ctgggtagcc cacgtcccac
      781 aggcacaggc cgtgggcggg ggccaagcgg ggcgcgccac ggcggtcgcg ggcgcgcatg
      841 atgccgcgca cctcgcccgc gtgacgcacc ccggtgccga cgtcgatcat catccccacg
      901 atcgaacgca ccatctgctg gcagaacgcg ttggcccgga tctcgaagcg cagcacgcgc
      961 tggtcggtga acgcggtgaa ctcgctgctc acatcgctcc agcnnnnnnn nnnnnnnnnn
     1021 nnnnnnnnnn agcggtgcat gctcggcggc ggctggtcgg ggtcgcggtc gggcacccgg
     1081 cagaacgagg tgaagtcctg ctcgccgatc aacgggtcgc aggccaactg catcgcgggc
     1141 agcgagagcg ggcggtgcat ccgccacgcg gtcgcctcca agaacgggtt gggctgggcc
     1201 gcgttgagca ccgtgtagcg gtagtggcgc cacagcgcgc tgaagcgggc atggaaggtg
     1261 tcgccggccc actccgcgga acggaccacg acctccggtc cgcacatcgc gttggcccgt
     1321 cgcacgacgc cggtgaggtc ggcatcggcc gggatgtcgc ccgacaccac ctgaccccag
     1381 ccgtgcaccc ccgcatcggt gcgacccgcg ccggccaact cgaccgggtg acgggtgatg
     1441 gtgctgagcg cgtcggtgag cacacccatc acggtgcgca cctggtggtt gatcgcgaag
     1501 ccacgaaagc cgcggccgtc gtaagcgaca acgagacgaa cccgccgcag cgcgacgggt
     1561 tcgttcagag ctgtgtcgat gccgtcgagc ggcaatgtgt cagaccagtt cgatgcgggc
     1621 catcggcgcg ttgtcgccct ggcgcggacc cagcttgagg atgcgcaggt acccgccgtt
     1681 gcgctcggtg taccgggggg cgacgtcgtt gaccagcttg tgggtcatct cgcggtcgcc
     1741 caggtacccc tggatctggc ggatgcggtg cacccgcatc gggtcggcgt caccggcctt
     1801 cttggccttg gtgatcagct tctccgcaat ggggcggagg gccttggcct tggcctcggt
     1861 ggtgacgatc gcctcggcgg cgatcagcga tgcggccagg ttggccatca tcaacttctg
     1921 gtgctgcgag ctgccgccga agttgggggc tcgacgtggt gttgccggca tgagagtctt
     1981 ccttgattcg cgagtggtca gccgcgcagc gtgaggccgc gctcgtcgag cttctgcttg
     2041 acttcgtcga ggctcttctg accgaagttg gtgatgttga gcaggtcgtc ctcgttcttg
     2101 gtgagcaact caccgatcga gttgatctgg gcccgcttga ggcagttgcg aggacgctcg
     2161 gacaggtcga ggtcttcgat cggcaggtcg aggtcgggcg aggtcgcgct ggtggcggcc
     2221 acttcaccga gctcgagacc ctggggctct tcgctgaggt tggcgacgag gtcgaccaac
     2281 gaacgcagcg tggcaccggc cgaagccagc gcgtcacgcg gggcgacgct gccgtcggtc
     2341 tcgatgtcga gcacgagacg atcgaagttg gtgctctgct cgacacgggt cggttcgacc
     2401 tcgaaggtca cccggcggac cgggctgaag atcgcgtcga ccggaatgac gccgatgact
     2461 cgcgagtcgc tgtcgcggtt gctgctgagg tagccgcgac cacgctcgac cgtgagatcg
     2521 atcgccagac ggcccttggc gttgatcgac gccaggtgca ggtcgccgtt gaggatctcg
     2581 acctcgctgg ggcacttgat gtcggctgcc gagacctcgg ccggaccacg cacgtcgaga
     2641 cggatgatca cctgctcgtc gctggtgctc gtgagcacga tgtccttgat gttcaagatg
     2701 acatcggtga tgtcctcggc aacaccctgg atggtgtcga actcgtgcag ggcgtcgtcg
     2761 aagcgcacgg ccgtgacggc cgcacccggg atcgacgaca gcagcgtgcg acggagcgag
     2821 ttaccgatcg tgtgaccgaa gccaggctcg agcggcccga tcgcgaaacg ctgccggttg
     2881 ttggattctt cgccgatggc ctcgacggtg gggcgctgga tgacgagcat gtggatgcct
     2941 cggacccttt ctgaacggac tggcagcgat tacttggagt agagctcgac gatgagctgc
     3001 tcgcgcacgg gcacgtcgat gtgctcgcgc tgaggcaggt cgcgcacggt gacttgcttg
     3061 ccaccgtcgc ccacctcgag ccagccgacg acgcggcggt cgagcacgtc gaggttgtgc
     3121 tggaccacga tcatgttgcg ggccttctcg gacaggctga cgacgtcgcc gcgcttgacg
     3181 cgggcgctgg ggatgtcgag ccgcttgccg ttgacgagaa tcttgccgtg gctcacgagc
     3241 tggcgggcct gcgggcgggt ctcggcccaa ccggcgcggt agacgatgtt gtcgatgcgc
     3301 tgttcgagca ggcggagcag gttctcgccg gtgggaccct ggaggcggtt ggcctcgtcg
     3361 taggtcttgc ggaactgccg ctcgagcacg ccgtagatgt acttggcctt ctgcttctcc
     3421 tgcagctggg cgaggtactc gctgccggcg ttgcggcggc gggtgcgacc gtgctgaccc
     3481 ggggggaagg ggcgacgctc gagcgccttg cggcccttct cgttctcgaa gatgttggtg
     3541 gacagacggc gcgagacgcg caccttggga ccggtgtaac gggccatggc tcagctcctc
     3601 ttccgcttcg gctggctgca accgttgtga gccaacggtg tgacgtcctt gatcgaggtg
     3661 acttcgagac cggtgttctg gatggaacgc acggcggtgt cacggcccga acccgggccc
     3721 ttgacctgta cctcgacacg gcgcaggccg tgctccatcg cggcgcgggc ggccttgtcg
     3781 gcagccatct gcgcggcgaa cggggtgctc ttgcgcgacc ccttgaaccc aaccccaccg
     3841 gagctggccc acgagatgac attgccctcg gtgtcggtga cgctgacgat ggtgttgttg
     3901 aacgagctct tg
//
LOCUS       DAORNF010000281         3886 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_17538_length_3886_cov_1.838162, whole genome shotgun sequence.
ACCESSION   DAORNF010000281 DAORNF010000000
VERSION     DAORNF010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3886
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17538_length_3886_cov_1.838162"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="PLV68_11635"
     CDS             <1..370
                     /locus_tag="PLV68_11635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01942.1"
                     /translation="QDDPDARRAAFWATGFAIFTFWNLGTLVGALVGNGLDPQTFGLD
                     AAFPAAFVAMLWPHLRSRRGRLAAGIGAAVCLALIPFVPVGVPILCASLAVLIGIPAL
                     PDAALDVGLDDDITEIGPVP"
     assembly_gap    424..499
                     /estimated_length=76
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(694..1677)
                     /gene="galT"
                     /locus_tag="PLV68_11640"
     CDS             complement(694..1677)
                     /gene="galT"
                     /locus_tag="PLV68_11640"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HRE01943.1"
                     /translation="MSQLRLNPLTGRWVTIVAERAERPTDFAPRSAPVEADPSRPCPF
                     CPGNEEATLPALEQIGADGNWQLRVIPNLYPAFDGDEPFAVHHLGPVHVIAEASGIHE
                     VFVYTPDHDGGLELSDDAKVAQVMQALKRRFVDHAAVPNIRYTQAIINHGREAGASLA
                     HPHGQLLGLPFVPGEIIEEERSFARFESACILCASVEAELVEGTRVLFATDDAVCISP
                     FWAGSPYELLIIPRRHDEHLQDASDVSLTAVGVAIRDAVLNLQELFGDVAYNLVFHPA
                     PHRHAGAFHWHVHLFPKLVTTAGFERGTGVHLNIVPPEVAADKLRRVAINH"
     gene            complement(1750..>2435)
                     /locus_tag="PLV68_11645"
     CDS             complement(1750..>2435)
                     /locus_tag="PLV68_11645"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRE01944.1"
                     /translation="DIDATVALLVSLAAPSVPAPVVELGSGTGRLAFPLARAIGERTL
                     IAIDSSPEMLAVAERKGPPSNVVTITGDMVEVLRGPDVPAAGLVFVAYNTFFNLTAPG
                     AQQACFDAVAARLAPGGCFVIEAFVPDDRGAQRDHVEVRSMTAAHVVLSISRFDGDHG
                     AAGQFVEFSEKSGIRLRPWHIVYASPAELDAMAARAGLTLHARWEDTARTPFTPDSQR
                     HVSVYTPPT"
     assembly_gap    2436..2497
                     /estimated_length=62
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2560..3534
                     /locus_tag="PLV68_11650"
     CDS             2560..3534
                     /locus_tag="PLV68_11650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01945.1"
                     /translation="MSAWAGIESDNDSTAASGRPPATVAATPVPRTARLRRATQPLLE
                     SFDTAGVMFQRWARPLLIGTAVIMVPTAAVQVIGAAMAFDRYADFSGAPVALPLWGDG
                     AASRGVEDVVRFVGLVTASMAAALTGGFVAPLVARYRYGMAVRVGDGLRPMVRRLPAL
                     VVAWALGHCWIVLAGWVAGSLEGSTRWMVAVFVAPVLAWIVAVTLTVVPVMMLEARGP
                     LAGLRRAAQLYRRAPGMAFGFVVLGAPLGAFLHYGLAVLPRLLQEVGLITFGRFGWLV
                     EGVAAQIGLLISAPVLAVATTLCYLEMRMQAEGMDLIAEADHVFPARR"
     gene            3531..>3886
                     /locus_tag="PLV68_11655"
     CDS             3531..>3886
                     /locus_tag="PLV68_11655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01946.1"
                     /translation="MTSVGAVLVGRWLTAADPLSPVDQDPDVVRDQACRLVESSSFCS
                     PPKAPTRLPEASTGGGAAVGIGSIILWIVLVVALATLVWVVLRAVAGRGGFRRRRTAG
                     EEDTEADDVVLDGEAT"
BASE COUNT          490 a         1194 c         1433 g          631 t
ORIGIN      
        1 ccaggacgat cccgacgcgc gccgggcagc gttctgggcc acggggttcg cgatcttcac
       61 gttctggaac ctgggcaccc tcgtcggcgc gctggtcggc aacggcctcg acccgcagac
      121 gttcggactc gacgcagcct tccccgccgc gttcgtggcg atgctgtggc cgcacctgcg
      181 gtcgcgccgt ggccgcctgg cggctgggat cggcgccgcg gtgtgtctgg cgctgatccc
      241 gttcgtaccc gtcggcgtgc cgatcttgtg tgcctcgctg gcggtactga tcggcatccc
      301 cgccttgccg gacgccgcgc tcgacgtggg tctcgacgac gacatcaccg agatcggacc
      361 cgtgccatga cctgggggtt ggtgctcggc ctcgccgccg gggcgtacgt cctgaaggtg
      421 ctcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      481 nnnnnnnnnn nnnnnnnnnc gtcgtcaagg acacgttcag caccggccag cacctggtgc
      541 tcgacccgag agtggtcggc gtcgccggcg ccgtcatcgc ggcatggcgc aagctaccga
      601 tgctcgccgt catcctgatc gccgcagtgc tcaccgcggc gacccgcgcc ctgacgggct
      661 gagctactga cgctgctgac tgggcgactg gcgtcagtgg ttgatggcga cgcggcggag
      721 cttgtcggcc gccacttcgg gcgggacgat gttgaggtgt actccggtgc cgcgttcgaa
      781 gccggcggtg gtgaccagct tggggaacag gtgcacgtgc cagtggaagg cgccggcgtg
      841 gcgatgcggg gcggggtgga acaccaggtt gtaggccacg tcaccgaaca gttcctgcag
      901 gttgagcacc gcatcgcgga tggcgacgcc caccgcggtc agcgacacgt cgctggcgtc
      961 ttgtaggtgc tcgtcgtgcc ggcgggggat gatcagcaac tcgtacgggc tgccggccca
     1021 gaacgggctg atgcagacgg cgtcgtcggt ggcgaacagc acccgggtgc cttcgaccaa
     1081 ctcggcctcg accgacgcgc acaggatgca cgcgctctcg aagcgggcga agctgcgctc
     1141 ttcttcgatg atctcgccgg gcacgaacgg caggcccagc agctggccgt ggggatgggc
     1201 cagcgatgcc ccggcttcgc ggccgtggtt gatgatcgcc tgcgtgtagc gaatgttggg
     1261 cacggcggca tggtcgacga agcggcgctt gagggcctgc atgacctggg cgaccttggc
     1321 atcgtcggac agttcgagcc cgccgtcgtg atcgggggtg tagacgaaca cctcgtggat
     1381 gccgctggcc tcggcgatga cgtgaaccgg gccgaggtga tggactgcga atggttcgtc
     1441 gccgtcgaat gcggggtaca ggttggggat gacgcggagt tgccagttgc cgtcggcacc
     1501 gatctgttcg agcgcgggca gggtggcttc ttcgttaccc gggcagaagg gacacggccg
     1561 actggggtcg gcttcgaccg gggcgctgcg tggcgcgaaa tcggtcggtc gctccgcccg
     1621 ctccgccacg atcgtgaccc agcgcccggt gagggggttc agtcgcaatt gagacactgc
     1681 aaccaggata gatcggaccg acccctccgg cgccgtcttg tcggaaatct cctctgacgc
     1741 gccgccgcgt caggtcggcg gggtgtagac gctgacgtgg cgctgactgt cgggggtgaa
     1801 gggggtacgg gcggtgtctt cccagcgtgc gtgcagcgtg aggcctgcgc gggcggccat
     1861 cgcgtcgagc tcggcgggtg atgcgtagac gatgtgccac ggccgcaggc ggatgccgga
     1921 tttctcgctg aactcgacga actgaccggc ggcgccgtgg tcgccgtcga agcggctgat
     1981 gctgagcacc acgtgggcgg cggtcatcga gcgcacctcg acatggtcgc gctgggcgcc
     2041 gcgatcgtcg ggaacgaacg cttcgatcac gaagcagccg ccgggtgcca ggcgggccgc
     2101 gaccgcgtcg aagcaggcct gctgggcgcc gggagcggtc aggttgaaga acgtgttgta
     2161 ggccacgaac accagcccgg cggcggggac gtcggggccg cgcagcacct cgaccatgtc
     2221 acccgtgatg gtgacgacgt tggacggcgg ccctttgcgc tcggcgacgg ccagcatctc
     2281 ggggctggag tcgatcgcga tcagggttcg ttcgccgatc gcgcgggcca ggggaaaggc
     2341 cagccgcccg gtgccgctcc ccaactcgac caccggcgca ggcaccgatg gggcggccag
     2401 cgagacaagc agcgcgacgg tggcgtcgat gtcggnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnggt cacggtatcc gcgacccgtc
     2521 caaccggcag cggagggccg ggtttgccag aatgccggga tgagcgcctg ggccgggatc
     2581 gagtcggaca acgactcgac ggccgcgtcc ggccgtcccc ccgccacggt ggcggcgact
     2641 ccggtcccgc gtacggctcg gttgcgccgg gcgacccaac cgctgctcga atcgttcgac
     2701 acggcaggcg tgatgttcca acgctgggcc cggccgctgc tgatcggtac ggcggtgatc
     2761 atggtgccga ccgcggcggt gcaggtgatc ggggcggcga tggcgttcga ccgctacgcc
     2821 gacttctcgg gcgcaccggt tgcgctgccg ctgtggggcg atggcgccgc gtcgcgcggt
     2881 gtcgaggacg tggtccgatt cgtcgggctg gtcaccgcca gcatggcagc agcgttgacg
     2941 ggcgggttcg ttgcgccgct ggtggctcgg tatcgctacg gcatggcggt gcgcgtcggc
     3001 gacgggttgc ggcccatggt caggcgcctc ccagcgctgg tggtggcgtg ggcgctgggg
     3061 cactgctgga tcgtgttggc cggctgggtc gccggatcgc tcgaaggttc cacacggtgg
     3121 atggtggccg tgttcgtcgc cccggtgctg gcctggatcg tggcggtcac gctgacggtc
     3181 gtgccggtga tgatgctgga ggcccgcggc ccgctggccg ggttgcgccg ggcggcgcag
     3241 ctctaccggc gcgcgccggg catggccttc gggttcgtcg tgctcggtgc cccgctgggt
     3301 gcgttcttgc actacggcct ggccgtgttg ccgcggttgc tgcaagaggt cgggctgatc
     3361 accttcggcc gcttcgggtg gctggtcgaa ggcgtggccg cgcagatcgg tctgttgatc
     3421 agcgcacccg tgctcgccgt ggcgaccacg ctgtgctacc tggagatgcg gatgcaggcc
     3481 gaaggcatgg atctgatcgc cgaagccgac catgtcttcc cggcgcggcg atgacgagcg
     3541 tcggtgccgt gctggtcggg cggtggctca ccgcggccga cccgttgagt cccgtcgatc
     3601 aggaccccga cgtggtgcgc gaccaggcgt gccgcctggt ggagtcgtcg tcgttctgca
     3661 gcccgcccaa ggcgccgact cgcctgccag aagcgagcac cggtggcggt gccgcggtcg
     3721 ggatcggcag catcatcttg tggatcgtgc tcgtcgtcgc gttggcgacg ttggtctggg
     3781 tggtgctgcg cgccgtggcc ggtcgcggcg gtttccgccg ccgtcgcaca gccggtgaag
     3841 aagacaccga ggcagacgac gtcgtgcttg acggcgaagc gactca
//
LOCUS       DAORNF010000282         3858 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_17718_length_3858_cov_1.691296, whole genome shotgun sequence.
ACCESSION   DAORNF010000282 DAORNF010000000
VERSION     DAORNF010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3858
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17718_length_3858_cov_1.691296"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(30..>770)
                     /locus_tag="PLV68_11660"
     CDS             complement(30..>770)
                     /locus_tag="PLV68_11660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01947.1"
                     /translation="AADSRNGLEAVDYLRATRGDTNTWVIALGTNDAAFYSTTQAMSV
                     IRTMLDRIGPGHRVIWVNIYLPKTPSWQASWNDALTRVADERPTELFVFDWASIAATR
                     AGIMNTDRVHMTTSGYSLRSQKIAEFTRSLPDPARPVALSTPTPAADGTAGGYVSLAP
                     SRVLDTRGGPKVASGAVVRVELGGVVPADASAVAINLTTTQADAPGYATAFPCDRAVP
                     ETSTVNYIADSTQSGSTLVFGGESHHVV"
     assembly_gap    771..806
                     /estimated_length=36
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<807..993)
                     /locus_tag="PLV68_11665"
     CDS             complement(<807..993)
                     /locus_tag="PLV68_11665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01948.1"
                     /translation="MTRLRAIALAMMVALIGSSLDVPAASTTASAAYSENSISMVGDS
                     LTYGSLPWQANAYRSAGW"
     assembly_gap    1175..1236
                     /estimated_length=62
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1343..>1984
                     /locus_tag="PLV68_11670"
     CDS             1343..>1984
                     /locus_tag="PLV68_11670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01949.1"
                     /translation="MDTPALDQLRDAVAAVLALDTTVFTAAELEAVRVAVTKAHAGLG
                     VATAQIDRDWLARGAWSVDGSKTPAHRLANQTCRSRHVLQGELVRAVGLDDLPQVVVD
                     AVVAGEVPIEVLDACIAANTPARRELFHRDADVILNGCRGLLWYQARRVIRHWVNRAD
                     DALTDPTKVSDPPPVPECGYLFASKTTGKVVFNGELPEIPGAIVTGELDRIADE"
     assembly_gap    1985..1999
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2108..>2432
                     /locus_tag="PLV68_11675"
     CDS             2108..>2432
                     /locus_tag="PLV68_11675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HRE01950.1"
                     /translation="MFVVRVGDGAFGHLCELANGVPIGPGELVPYLSEAMVQSVLFDG
                     PITLLGVSKQRSFTGALRTAIAARDRHCQHPSGCDTPADLCDVDHIIPWSQGGPTAAW
                     NGRMEC"
     assembly_gap    2433..2471
                     /estimated_length=39
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2729..>3858)
                     /locus_tag="PLV68_11680"
     CDS             complement(2729..>3858)
                     /locus_tag="PLV68_11680"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HRE01951.1"
                     /translation="FGGNYRIIDFALSNFANSGFLKIVVLTQYKSHSLDRHISQTWRF
                     STLLGNYVTPVPAQMRRGPQWFSGSADAIYQNLNLISDERPDLVCVFGADHIYHMDVR
                     QMVDHHLDSGAGVTVAAIPVPIDQADQFGIIHADVNGKIVQFLEKPADPPSMPGDPTR
                     ALASMGNYVFDTQTLIDIVSPTGDDAIATDIGGDVIPALTNQGVAHMYDFSTNVIPGQ
                     ADHERGYWRDVGSLDAYYEASMDLLRPVPPFSLYNEKWPVYSIQLPLPPAKLDHGPGG
                     EPAVVGNSLLSAGSIVSGGSVHRSILFPETYIDASAEVTESILFPGVRIGPGARVHRC
                     VIDKNVVVPAGHQIGYDAEADRAQYVLSDHGVVVVPKGRLV"
BASE COUNT          644 a         1092 c         1293 g          677 t
ORIGIN      
        1 accatcaccc cgaggatgcc ggcccgctcc tacaccacgt gatgggactc acccccgaac
       61 accagcgttg aacctgactg agtcgagtcg gcgatgtagt tgaccgtgga ggtctccgga
      121 accgctcgat cacaggggaa agccgtggcg taccccggtg cgtcggcctg agttgtggtc
      181 aggttgatcg ccactgccga cgcatctgct ggcaccacgc caccgagttc gactcgcacg
      241 actgctccgg acgccacctt ggggccaccc cgagtgtcga gcactcggct cggagcgagc
      301 gagacgtacc caccggcggt gccgtcagca gcgggggtcg gagtgctcag ggcgaccggt
      361 cgtgccgggt caggaagcga ccgggtgaac tcggcgattt tctgggaccg cagcgagtac
      421 cccgaagtgg tcatgtggac gcggtcggtg ttcatgattc ccgcccgcgt tgccgcgatc
      481 gacgcccagt cgaagacaaa cagttccgtt gggcgctcat cagccactcg cgtcagagcg
      541 tcgttccacg atgcctgcca cgatggcgtc ttgggtaggt agatgttcac ccagatgact
      601 cgatgcccag ggccgatccg atccagcatc gttcggatga cgctcatcgc ctgggtggtg
      661 gagtagaacg ccgcgtcgtt cgtaccgaga gcgatcaccc acgtgttggt gtcaccgcga
      721 gtggctcgga ggtagtccac cgcctcgagt ccattccggg aatctgcggc nnnnnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn nnnnnnccca gccagccgag cgataggcgt tggcctgcca
      841 cggcagagag ccgtaggtga gtgagtcgcc caccatgctg atgctgttct cggagtaggc
      901 ggcagaggcg gttgtactcg cagcgggcac atcgagtgag ctaccgatca gggcgaccat
      961 catcgccagg gcgatggcgc gcagtcgggt catgttcata tctgcctacc gccggtcgat
     1021 atcgagaaag gtatgtggat cgaacacgag aagtgatgct gaagaaggcg acccggattc
     1081 tccgccgaga tccgacccgc agaaccacgc aggcgagatg aaatcgccaa gtatttgcac
     1141 cttctgtgtc aaatcgttag tacaagggga ctggnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnatct gatgatcatc cgagacaggc
     1261 ctccttaccg tccacaggaa aggtcctatg gacgaacgac agttcggctc actgtgtaac
     1321 acccctttgc gatgatggcg gtatggacac gccagcgctc gaccaactcc gcgatgcggt
     1381 cgcggcggtg ctcgcgttgg acacgaccgt gttcaccgcc gcggagttgg aggcggtccg
     1441 ggttgcggtg acgaaggctc atgcgggtct tggtgtggca acggcgcaaa ttgaccgcga
     1501 ttggttggct cggggtgcgt ggtcggtgga tgggtccaag actccggcgc accggttggc
     1561 caaccagact tgccggtcgc gccatgtgtt gcagggtgag ttggtccgcg ctgtcggctt
     1621 ggatgattta ccgcaggtgg tcgtggatgc ggtggttgct ggtgaggtgc cgatcgaggt
     1681 gttggacgcg tgtatcgcgg cgaacacgcc ggcacggcgt gagctgttcc atcgtgatgc
     1741 cgacgtgatt ctcaacgggt gccgcggctt gttgtggtat caagctcgcc gagtcattcg
     1801 ccactgggtc aaccgggccg atgacgcgtt gaccgaccca accaaggtga gcgatccacc
     1861 tccggtacca gagtgtgggt atctgttcgc gtccaagacc accggcaagg tggtgttcaa
     1921 cggcgaactc ccagaaattc ccggcgcgat cgtgaccggt gagttggacc gcatcgctga
     1981 tgagnnnnnn nnnnnnnnng cggccggtgt cacccggacc gattcacaac gtcgcgctgc
     2041 cgctttggtc gaaatggccc gtcgctccct cagtctccgc aacggcgcgg gcggcacagt
     2101 cgccccgttg ttcgtcgtgc gagtcggcga cggcgcgttc ggtcatttgt gtgagttggc
     2161 gaacggtgtc ccgatcgggc ctggcgaatt ggtgccgtat ctgtccgagg caatggtgca
     2221 gtcggtgctg ttcgacgggc cgatcaccct gctcggcgtg tccaagcaac gcagcttcac
     2281 cggtgcgttg cggactgcga tcgccgcccg tgaccggcac tgccaacacc cctccggttg
     2341 cgatacccca gcagacctgt gcgacgtcga ccacatcatc ccctggtcac aaggcggccc
     2401 gacggcggcg tggaacggcc ggatggagtg ccnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn ntacccggag caccacccga tcgaagcgga catcttgaag gcgaaagccg
     2521 cttggcagct acgagagcac caccgccgca ctcgcggcgc agagcgtggc ggtgatggtg
     2581 gtggcagcgc tgccgacagc gacggttcat ctgactccgc cgcctgacgg aagtgagagc
     2641 agctatcgag ccgtggacgc tggcctgcgc ggtcttgccg ccgggggagc ggcggtggtc
     2701 gggcgggggt ggcgcatggt aggtggggct agacgaggcg gcctttgggg accacgacga
     2761 cgccgtggtc gctgagaacg tattgggcgc ggtcggcttc ggcgtcgtag ccgatctggt
     2821 ggccggcggg gacgacgacg ttcttgtcga tcacgcagcg gtggacgcgg gcgccggggc
     2881 cgatgcgcac gccggggaac aggatcgact cggtgacctc ggccgaggcg tcgatgtagg
     2941 tctcgggaaa caagatcgaa cgatgaacac ttccacctga cacgatcgat ccagcggaga
     3001 gcaagctgtt gccgacgacg gcgggctcgc caccgggtcc gtggtcgagc ttggccgggg
     3061 gcagggggag ctggatgctg tagaccggcc acttctcgtt gtacaagctg aacgggggca
     3121 ccggccgcag caggtccatg ctcgcctcgt aataggcgtc gaggctgccg acatcgcgcc
     3181 agtagccgcg ctcgtggtcg gcctggccgg ggatgacgtt ggtggagaag tcgtacatgt
     3241 gggcgacccc ctggttggtg agggccggga tgacatcgcc accgatgtcg gtcgcgatcg
     3301 cgtcgtcccc ggtggggctg acgatgtcga tcagcgtctg ggtgtcgaac acgtagttgc
     3361 ccatcgacgc tagggcacgc gtcggatcac cgggcatcga ggggggatcg gccggcttct
     3421 ccagaaactg cacgatcttg ccgttgacgt cggcgtggat gatgccgaac tggtcggcct
     3481 gatcgatcgg cacggggatt gcagcaaccg tgacaccggc gccgctgtcg agatgatggt
     3541 cgaccatctg gcgcacgtcc atgtggtaga tgtgatcggc accgaacacg cagaccagat
     3601 cggggcgctc gtcgctgatc aggttgaggt tctggtagat cgcatcggcc gaaccgctga
     3661 accactgcgg tccacggcgc atctgggccg gcaccggggt gacgtagttg ccgagcagcg
     3721 tgctgaaccg ccaggtctgg ctgatgtgac ggtcgaggct gtggctcttg tactgcgtca
     3781 gcacgacgat cttgaggaat cccgagttgg cgaagttgga cagcgcgaaa tcgatgatgc
     3841 ggtagttgcc gccgaacg
//
LOCUS       DAORNF010000283         3858 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_17719_length_3858_cov_1.572180, whole genome shotgun sequence.
ACCESSION   DAORNF010000283 DAORNF010000000
VERSION     DAORNF010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3858
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17719_length_3858_cov_1.572180"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>433)
                     /locus_tag="PLV68_11685"
     CDS             complement(<1..>433)
                     /locus_tag="PLV68_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HRE01952.1"
                     /translation="FVVLSGAAIGFLGFGVWAHHMFASGLGPVSVAVYSAGSMAIAIP
                     TGVKIINWSLTLWGGKIRFATAAKFAIGLIVLFTIGGLSGVTHAVSPSDTQQTDTYYI
                     VAHFHYVLFGGAIFGLFAGFYYWWPKIFGKMLNERLGKWNF"
     assembly_gap    434..506
                     /estimated_length=73
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<507..849)
                     /locus_tag="PLV68_11690"
     CDS             complement(<507..849)
                     /locus_tag="PLV68_11690"
                     /inference="COORDINATES: protein motif:HMM:NF012343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HRE01953.1"
                     /translation="MYAPNSSSSFSPTHGVDFWALGLLITGISSLTGAVNLIVTVLNM
                     RAPGMTIMRVPTLSWMTLVTQTILLFAMPVITVALVLLMLSRNFGATFFTVDKGADPL
                     LWQHLFWIFGHP"
     assembly_gap    890..899
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<900..1369)
                     /locus_tag="PLV68_11695"
     CDS             complement(<900..1369)
                     /locus_tag="PLV68_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HRE01954.1"
                     /translation="MIHPTPALEPSTLDAPARQPPALPRSDAGVTPEQALGIYRRPLH
                     TTGWRSWLVTVDHKRIGIMYGVAAFAFFIVGGFEALLIRIQLAAPDNKVLSADAYNQM
                     FTMHATSMIFLFAMPMAAALGNYFVPLQIGARDVAFPRLNLFSFWCYLTGALFL"
     gene            complement(1390..1815)
                     /locus_tag="PLV68_11700"
     CDS             complement(1390..1815)
                     /locus_tag="PLV68_11700"
                     /inference="COORDINATES: protein motif:HMM:NF017426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF779 domain-containing protein"
                     /protein_id="HRE01955.1"
                     /translation="MSPPVERVAITHAAAQMLASLVEQHGPVLFHQSGGCCDGSSPMC
                     FPRGEFRIGDSDVYLGAIEFAEGVEPAPFYMGAAQFEYWAHTHLTVDVVPGRGAGFSL
                     EAPEGVRFIIRSRLFTDDESAELARHGPPPTGAYFSTVQ"
     gene            complement(1815..>2655)
                     /locus_tag="PLV68_11705"
     CDS             complement(1815..>2655)
                     /locus_tag="PLV68_11705"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRE01956.1"
                     /translation="LASSSRIAKIAFTGETTTGRLIMQYASENIIPVTLELGGKSPNI
                     FMPDVMEADDDFLDKAVEGFVMFALNQGEVCTCPSRALIHDSIYDAFMERALQRTEAI
                     VTGHPLDPTTMIGAQASNDQYEKILSYIDIGRAEGAEVLTGGSSRGVEGLDGGFYIEP
                     TILRGTNSMRVFQEEIFGPVVAVTTFANEAEAIRIANDTLYGLGAGLWTRDGSAAYRM
                     GRAIQAGRVWTNCYHAYPAHAAFGGYKGSGIGRENHSMMLDHYQQTKNLLVSYSPHRL
                     GFF"
     assembly_gap    2656..2733
                     /estimated_length=78
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2734..>2971)
                     /locus_tag="PLV68_11710"
     CDS             complement(<2734..>2971)
                     /locus_tag="PLV68_11710"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRE01957.1"
                     /translation="LPLAVDHFRYFAGAVRAQEGGISQIDNDTVAYHFHEPLGVVGQI
                     IPWNFPILMATWKLAPALAAGNCVVLKPAEQTPWS"
     assembly_gap    2972..3023
                     /estimated_length=52
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3024..3322)
                     /locus_tag="PLV68_11715"
     CDS             complement(<3024..3322)
                     /locus_tag="PLV68_11715"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRE01958.1"
                     /translation="MQFPAPGSAASSISVAERYDNYIGGKWVAPVNGRYFENISPVDG
                     KPICEIARSTAEDIDLALDAAHAAREAWGATSATERSNILLKIADRLEANLEALA"
     gene            3440..>3858
                     /locus_tag="PLV68_11720"
     CDS             3440..>3858
                     /locus_tag="PLV68_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HRE01959.1"
                     /translation="MRRQEKVERIGLILDDLYPEPPIPLDHVDPYTLLVAVALSAQTT
                     DKKVNQVTPALFAAAPDPAAMHALGPERILGYIREVGLAPTKAKNVWIAAGQILDAGG
                     EVLPDWQFLEGLAGVGHKTASVVMAQAFGVPAFPVDT"
BASE COUNT          664 a         1135 c         1241 g          605 t
ORIGIN      
        1 cagaagttcc acttgccgag ccgctcgttg agcatcttgc cgaagatctt cggccaccag
       61 tagtagaagc cggcgaacag accgaagatc gcaccaccga agagcacgta gtggaagtgg
      121 gcgacgatgt agtaggtgtc ggtctgctgg gtgtcggacg gtgacacggc gtgggtgacg
      181 cccgacaagc caccgatggt gaacagcacg atcaggccga tcgcgaactt tgccgcagtg
      241 gcgaagcgga tcttgccgcc ccacaacgtc aggctccagt tgatgatctt gaccccggtg
      301 gggatcgcga tcgccatgct gccggccgag tacaccgcca cgctgaccgg gccgaggccg
      361 gaggcgaaca tgtggtgggc ccacaccccg aagccgagaa agccgatcgc tgcacccgac
      421 agcacgacga acgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      481 nnnnnnnnnn nnnnnnnnnn nnnnnncggg gtgaccgaag atccagaaca ggtgctgcca
      541 cagcagcgga tctgcgcctt tgtcgacggt gaagaacgtg gcgccgaagt tgcggctgag
      601 catcagcagc accaaggcaa cggtgatgac cggcatggcg aacagcagga tcgtctgggt
      661 caccagggtc atccagctca gcgtcgggac gcgcatgatc gtcatgcccg gggcgcgcat
      721 gttgagcacg gtgacgatca ggttgaccgc gccggtcaac gacgagatgc cggtgatcag
      781 cagacccaac gcccagaagt cgacaccgtg ggtgggggag aacgagctcg acgagttggg
      841 cgcgtacatg aaccatccac cgtcgggggc cccgccgagg acccacgcgn nnnnnnnnnt
      901 tcaggaacag ggcgccggtc aggtaacacc agaagctgaa caggttgagg cgggggaagg
      961 cgacgtcacg agcgccgatc tggagcggca cgaagtagtt gcccagcgcg gccgccatgg
     1021 gcatcgcgaa caggaagatc atgctggtcg cgtgcatggt gaacatctgg ttgtaggcgt
     1081 cggccgacag caccttgttg tcgggtgcgg cgagttggat gcggatcagc agcgcctcga
     1141 acccgccgac gatgaagaac gcgaacgcgg cgacgccgta catgatgccg atccgcttgt
     1201 gatcgacggt gaccagccac gatctccaac cggtggtgtg cagtgggcgg cggtagatgc
     1261 cgagtgcctg ctcgggtgtg acgccagcgt ctgatcgcgg cagcgcggga ggttgccggg
     1321 ccggtgcgtc gagcgtcgac ggttcgagtg ctggtgtggg gtggatcatc gccacccacg
     1381 ttaatacttc tactgaacag tagagaaata tgctccggtc ggcggtgggc cgtggcgggc
     1441 gagttcggcc gactcgtcgt cggtgaacaa acgcgaccga atgatgaacc gcacgccttc
     1501 gggggcctcg agcgagaagc cagcgccgcg gccgggcacg acgtcgacgg tcaggtgggt
     1561 gtgggcccag tactcgaact gagccgcacc catgtagaac ggtgcaggct cgaccccttc
     1621 ggcgaactcg atcgcgccga ggtagacgtc gctgtcgcca atgcggaact caccgcgggg
     1681 aaagcacatc ggactggagc cgtcgcaaca accgcccgac tggtggaaca gcactggacc
     1741 gtgctgttcc accaggctcg cgagcatctg cgccgcggcg tgcgtgatcg cgacacgctc
     1801 gacaggcggg ctcatcagaa aaagccgagc cgatgcgggc tgtagctgac cagcaggttc
     1861 ttggtctgct ggtagtggtc gagcatcatc gagtggttct cgcggccgat gcctgaaccc
     1921 ttgtagccgc cgaacgcggc atgagccgga taggcgtggt agcagttggt ccacacccgc
     1981 ccggcctgga tcgcccggcc catgcgatag gccgcgctgc cgtcgcgggt ccacaatccg
     2041 gcccccaggc cgtacagcgt gtcgttcgcg atgcggatcg cctcggcctc gttggcaaac
     2101 gtggtgacgg cgacgaccgg tccgaagatc tcctcctgga agacacgcat cgagttcgtg
     2161 ccgcgcagga tcgtcggctc gatgtagaag ccaccgtcga gaccttcgac accgcgcgat
     2221 gagccgcccg tgagcacctc ggctccttcg gccctaccga tgtcgatgta cgacaagatc
     2281 ttctcgtact ggtcgttgga cgcctgcgcg ccgatcatcg tggtcgggtc gagcggatgt
     2341 ccggtgacga tcgcctcggt gcgttggagc gcccgctcca tgaaggcgtc gtagatcgag
     2401 tcgtggatca acgcccggct cgggcaggtg cacacctcgc cctggttgag ggcgaacatc
     2461 acgaagcctt cgaccgcctt gtcgaggaag tcgtcgtcgg cctccatcac gtcgggcatg
     2521 aagatgttcg ggctcttgcc gcccagttcc agcgtgaccg gaatgatgtt ctctgacgcg
     2581 tactgcatga tcaaacgacc ggtcgtggtc tcgccagtga aggcgatctt ggcgatccgg
     2641 ctcgacgatg cgagcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngaccacg gggtctgctc ggccggcttc
     2761 aacaccacac agttacccgc agccaaggcg ggcgcgagct tccaggtggc catcaggatc
     2821 gggaagttcc acgggatgat ctgacccacg acgcccagcg gctcgtggaa gtgataggcg
     2881 accgtatcgt tgtcgatctg gctgatcccg ccttcctggg cgcgcacggc gccggcgaag
     2941 tagcggaagt gatcgaccgc caacggcagg tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnatcgcca gcgcttcgag gttggcctcg agccgatcgg
     3061 cgatcttgag caggatgttg ctccgttcgg tcgcgctggt ggcaccccag gcctcgcgcg
     3121 cggcatgtgc cgcgtcgagc gcgagatcga tgtcctccgc ggtcgagcgg gcgatctcac
     3181 agatcggctt gccgtcgacg ggggagatgt tctcgaagta gcggccgttg accggggcga
     3241 cccacttgcc gccgatgtag ttgtcgtagc gttctgccac cgagatcgaa ctggcggcgg
     3301 aacctggtgc tggaaactgc atgatgcccc ccaaggcgtc gtggcacgga gtgctccgtg
     3361 ctctcccaga tgttcgtccg ttttctccgg caagtccagg tttctccggc aagtccaggg
     3421 gcagctacgg tcgcctgcca tgcgtcgcca ggagaaggtg gagcggatcg ggctgatcct
     3481 cgacgacctc taccccgagc caccgatccc gctcgaccac gtcgatccct acacgttgct
     3541 ggtcgcggtg gcactgtcgg cccagacgac cgacaagaag gtcaaccagg tgaccccggc
     3601 gttgttcgcc gccgcccccg acccggccgc gatgcacgcg ctcggcccgg agcgcatcct
     3661 ggggtacatc cgcgaggtgg gcctcgctcc gacgaaggcc aagaacgtgt ggatcgccgc
     3721 gggtcagatc ctcgacgccg gcggcgaggt gctgcccgac tggcagttcc tcgaaggctt
     3781 ggccggcgtc ggacacaaga ccgccagcgt ggtcatggct caggcgttcg gggtgcccgc
     3841 ctttccggtc gacaccca
//
LOCUS       DAORNF010000284         3841 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_17843_length_3841_cov_1.325938, whole genome shotgun sequence.
ACCESSION   DAORNF010000284 DAORNF010000000
VERSION     DAORNF010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3841
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17843_length_3841_cov_1.325938"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /locus_tag="PLV68_11725"
     CDS             <1..663
                     /locus_tag="PLV68_11725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01960.1"
                     /translation="AMHHPYDTSTPIVGDGEAPAGMVRHVAWWNGLTTHEEMSRARFV
                     DAPICHPTAMMRRELVSYRAGPFAEDHDLWLRLLAENVRFAKVDETLVTWRERPERAT
                     RVDSRYDDEHRRALVHEHLVRELRSRRVVLWGAGEYGRWHGRHLRAAGVVIEQALDID
                     PKKVGRRILDGVPVESADHLGPPDGRCVLAAVASRGAQSEIRERLESAGWREGVDWWA
                     LQ"
     gene            682..>1049
                     /locus_tag="PLV68_11730"
     CDS             682..>1049
                     /locus_tag="PLV68_11730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01961.1"
                     /translation="MAVLDAPAGASYPGSSRLESVSPTDRPFAMEPHMNRRLTLAWLS
                     SLLLTALALVPSDAFAAAKRVLFVTINGGYNGDGVNMYNELNTYVTATHGVEGQVDYL
                     LLNADGLVTAALTAHTYEQI"
     assembly_gap    1050..1133
                     /estimated_length=84
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1134..>1384
                     /locus_tag="PLV68_11735"
     CDS             <1134..>1384
                     /locus_tag="PLV68_11735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01962.1"
                     /translation="KEVICDGRFLSSFWFGRSGSEGRLVTQNYYYNLMIRGGGILLAT
                     DHNDFANAGMNVLANLLGFGNFIGNFGGAFPLDAGHPLT"
     assembly_gap    1385..1420
                     /estimated_length=36
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1421..>2143
                     /locus_tag="PLV68_11740"
     CDS             <1421..>2143
                     /locus_tag="PLV68_11740"
                     /inference="COORDINATES: protein motif:HMM:TIGR02232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01963.1"
                     /translation="QAPFGLQPGGRTLRTIGYHSGNALNPGISTTIDGGVLGITVDIT
                     EEGGLVCEGSRTFNASITQGAEFGPFSYEWRVNNQVVGTGTSYTFDADLSGEGAFEVK
                     VIAQGAGLRADDDLVNLTVGGDGCVRCGNENLDEGEACDDGNIDDGDGCSTDCRIELG
                     GGCEVDGECVAIGVCFDGTCIARCYGNGDCDDENECTADLCDVESGLCSSGPREVGVA
                     CDGGVCDGEGGCETQTDPQNCG"
     assembly_gap    2144..2282
                     /estimated_length=139
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2525..3091
                     /locus_tag="PLV68_11745"
     CDS             2525..3091
                     /locus_tag="PLV68_11745"
                     /inference="COORDINATES: protein motif:HMM:NF014544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HYR domain-containing protein"
                     /protein_id="HRE01964.1"
                     /translation="MARAVRTETAARRATPVSRASVSVTTSPARRRASASASAPATRE
                     RVLAGDEGALAKGCIECEGDQTSPTIVCPAVVRNVECAQGGVTLGLPRPSAADDCSNP
                     TILSDAPPTYGVGVTNVTFTAVDGAGNRSSCTTAVEVRDTEPPTLFCPDRVTVEGDRG
                     ICGAYVDALIDQQVAVNTAAAKVQGLPG"
     gene            3088..>3841
                     /locus_tag="PLV68_11750"
     CDS             3088..>3841
                     /locus_tag="PLV68_11750"
                     /inference="COORDINATES: protein motif:HMM:NF020811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HRE01965.1"
                     /translation="MSEPSFMARFGAQLVDNGYHVIPIMPGSKTPGRFTRGRWLPYTG
                     WTKHADRATTLDEVEIWSSWPCCGIGIATGTTVALDIDVPDATIAHALTDLATEHLGA
                     TPFLRIGQAPKRLLLYRTDTPFAGRKRAPLEVLAHGQQFVAYAVHPGTGRPYAWPDEN
                     PLDVPWDTLPVVTEEQVLAWLDAAHAALPHELKPATLAGPATPSAWRGPSDPRGTVEA
                     VAAALTWLPNDDLDGHSWITIGNAIKAALGEEG"
BASE COUNT          579 a         1259 c         1212 g          532 t
ORIGIN      
        1 gcgatgcacc atccgtacga tacatctaca cctatcgtcg gcgacggcga ggcgccggcc
       61 ggcatggtgc ggcacgtcgc gtggtggaac ggactcacga cccacgagga gatgagccgc
      121 gcgcggttcg tcgatgcgcc gatctgccac ccgaccgcca tgatgcggcg cgagctcgtg
      181 agttatcggg ccggtccgtt cgccgaagac cacgacctct ggctgcggct cctggccgag
      241 aacgtccggt tcgccaaggt cgacgagaca ctcgtgacct ggcgggaacg accggagcgg
      301 gcgactcgag tcgacagccg ctacgacgac gagcatcgcc gggccctcgt ccacgaacac
      361 ctcgtccgcg agctccggtc acgtcgggtc gtgctctggg gcgcgggcga gtacggccgt
      421 tggcatggcc gacacctgag agccgcgggc gtcgtgatcg agcaggccct cgacatcgac
      481 ccgaaaaagg tcggtcgtcg catcctcgac ggagtgccgg tcgagtcggc ggaccacctc
      541 ggaccgcccg atggccgctg tgtgttggcg gccgtcgcga gccggggcgc acagtccgag
      601 atccgggaac gcctcgagtc ggccggctgg cgtgaaggcg tcgactggtg ggcgctccag
      661 tagcagtggt gcggaccgga ggtggcggta cttgacgcgc ctgccggggc ctcgtatccc
      721 gggtcgagcc gtttagaaag cgtctcccct accgaccgtc cgttcgcgat ggagccccac
      781 atgaaccgtc gtctcaccct cgcgtggctc tcgagccttc tgctgacggc cctcgccctc
      841 gtcccgagcg acgcgttcgc cgcggccaag cgcgtgctct tcgtcaccat caacggcggc
      901 tacaacggtg acggcgtcaa catgtacaac gagctcaaca cgtacgtgac ggcgacccac
      961 ggggtcgagg ggcaggtcga ctacctgctg ctcaacgccg acgggctcgt cacggcggcg
     1021 ctgaccgctc acacctacga gcagatctgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncaaggag
     1141 gtcatctgcg acggtcgctt cctgagctcg ttctggtttg gccgctcagg cagcgagggg
     1201 cgcctcgtca cccagaacta ctactacaac ctgatgattc gcggcggcgg catcctgctt
     1261 gcgaccgacc acaacgactt cgccaacgcg ggcatgaacg tgctggcgaa cctgctcggt
     1321 ttcggcaact tcatcggcaa cttcggcggc gccttcccac tcgacgcggg ccacccgctc
     1381 accannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gccaggcccc cttcggcctc
     1441 cagcccggcg gccgcacgct tcgcaccatc ggctaccact cgggcaacgc gctcaacccc
     1501 ggcatctcga ccaccatcga cggtggtgtg cttggcatca ccgtcgacat caccgaagag
     1561 ggcggcctcg tgtgtgaggg ctcgcgcacc ttcaacgcct ccatcacgca gggcgccgag
     1621 ttcggcccgt tcagctacga gtggcgcgtc aacaaccagg tcgtcgggac cggcacgagc
     1681 tacaccttcg acgccgacct gtccggcgaa ggcgccttcg aggtcaaggt catcgcgcag
     1741 ggcgcggggc tgcgcgccga cgacgacctc gtcaacctga ccgtgggcgg cgatggctgt
     1801 gtgcgctgcg gcaacgagaa cctcgacgag ggcgaggcct gcgacgacgg caacatcgat
     1861 gacggcgacg ggtgcagcac cgactgccgc atcgagcttg gcggcggctg cgaagtcgac
     1921 ggcgagtgtg tcgccatcgg tgtctgtttc gatgggacct gcatcgcgcg ctgctacggc
     1981 aacggcgact gcgacgacga gaacgagtgc acggcggacc tctgcgatgt cgagtcgggt
     2041 ctgtgttcga gcggtccgcg cgaggtcggt gtcgcctgtg acggcggggt ctgcgacggt
     2101 gagggcggct gcgagacgca gaccgacccg cagaactgcg gggnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nngtgtggcg aagggacctg cgccggtcag ggcacgcgca cctgtgtcga cgacgagttc
     2341 gtcacgagct gctcgcctcg gcccgacaat agcccgtgcg acgccggttt ctgcgcgacc
     2401 gccgcgacct gttcggccgg tgtctgccag gtgcggacct tccggaactg cgacgacaac
     2461 aacagctgca ccacggaggc gtgtgacccg atcgccggtc gctgcgtcgt cacccccgtc
     2521 accaatggca cgagctgttc ggaccgaaac ggctgcacga cgggcgacac ctgtgtcgcg
     2581 ggccagtgtg tcggtgacca cgtcacctgc gcgccgccgg gcgagtgcca gcgcgtcggc
     2641 acctgcaacc cgggaacgtg tccttgccgg tgacgagggc gcgctggcaa agggctgcat
     2701 cgagtgcgaa ggcgaccaga ccagccctac catcgtgtgc cccgcggtcg tgcgcaacgt
     2761 cgagtgtgcc cagggcggcg tgaccctggg gctgccgcgg ccgagcgcgg ccgacgactg
     2821 ctcgaacccg acgattctga gcgacgcgcc gcccacctac ggggtcggag tgaccaacgt
     2881 gaccttcacg gcggtcgatg gggccggcaa ccggagcagc tgcacgaccg ccgtcgaggt
     2941 gagagacacg gagccgccga ccctcttctg ccccgaccgc gtgacagtcg agggcgaccg
     3001 cgggatctgc ggcgcctacg tcgacgcgct gatcgaccag caggtcgcgg tcaacacagc
     3061 ggccgccaag gtgcaggggc tgccgggatg agcgagccga gcttcatggc ccggttcggc
     3121 gcccagttgg tggacaacgg ctaccacgtg atcccgatca tgcctggcag caaaacgcca
     3181 ggtcggttca cccggggccg ctggctcccc tacaccggat ggaccaagca cgccgatcgc
     3241 gccaccacgc tggacgaggt cgagatctgg tcatcctggc cgtgctgtgg aatcggaatc
     3301 gccaccggca ccacggtcgc gctcgacatc gacgtgcccg acgccacgat cgcacacgcc
     3361 ctcaccgatc tcgccaccga gcatctgggg gcgacgccgt tcctgcggat cggccaggcg
     3421 ccgaaacggc tgctgctcta ccgcacggat acgcccttcg ccgggcgcaa gcgggccccg
     3481 ctcgaagtgc tcgcccacgg ccagcagttc gtcgcctacg ccgtccatcc tggcaccggg
     3541 cgtccctatg cctggcccga cgagaacccg ctcgacgtgc cctgggacac gctgccggtg
     3601 gtcaccgagg agcaggtgct ggcatggctc gatgccgccc acgccgcgct gccgcatgaa
     3661 ctgaagcccg cgacgctggc tggccccgcc accccatccg cctggcgcgg cccgtccgat
     3721 ccccgtggca ccgtcgaagc cgtcgccgcg gcgttgacct ggcttccgaa cgatgatctc
     3781 gatgggcatt cctggatcac catcggcaac gccatcaagg cggcgctggg cgaggagggg
     3841 c
//
LOCUS       DAORNF010000285         3836 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_17883_length_3836_cov_1.978842, whole genome shotgun sequence.
ACCESSION   DAORNF010000285 DAORNF010000000
VERSION     DAORNF010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3836
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17883_length_3836_cov_1.978842"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>405)
                     /locus_tag="PLV68_11755"
     CDS             complement(<1..>405)
                     /locus_tag="PLV68_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HRE01966.1"
                     /translation="GGNLPGVVVASAMQAVISGGDSPALILFPQNTEGRDVVSRLSVK
                     LDKTVLTNNVNIEAAGDSFAVTTPIFGGNTLVTTTFTGSGPFLAAFRPKSFAAESAGG
                     GAPEVVSAPVGDLGAAGGAKVTAVHVEESSGP"
     assembly_gap    406..440
                     /estimated_length=35
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(584..1360)
                     /locus_tag="PLV68_11760"
     CDS             complement(584..1360)
                     /locus_tag="PLV68_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HRE01967.1"
                     /translation="MNVIVCVKQIPDPAAPGALDPSTNTLKRDGKLILDESDSYGVEM
                     ALQLVDKAGGGEVILVSMAPNGETAGLRTALAMGAAKGVLVSDPALQGSDALTTAKVL
                     AAAIQRAGGFDLVIGGTESSDGYTGTVPEQIAEVLGLPSVTFAKSLAIADGTLSAQRQ
                     TEAGYDDVTCSLPAVVSVTAGVVEPRYPSFKGIMAAKSKPIDTVTAADLGVTPVGWAG
                     AGQSITEVAQAEARAAGTIVEDDGEAFGQIVAYLENLKVI"
     gene            1434..2411
                     /locus_tag="PLV68_11765"
     CDS             1434..2411
                     /locus_tag="PLV68_11765"
                     /inference="COORDINATES: protein motif:HMM:NF012981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HRE01968.1"
                     /translation="MRIRLVVNAFASSVTARNTVVVHQVLSRGNDVDVVETNRRGHAT
                     RFSQDAARQGFDVVIGYGGDGTLNEVATGVAGTNTALGVLPGGSTNVFARTLGMPNDP
                     VEAVHLLAAGIARNDIEPIGLGHVNGRYFCFHTGVGFDAAVVNHVERHGSLKRWAGHP
                     LFIYAAMRTMANYDRTQPHFEVITSDSLDSSISRVSTSDAATGRDRDPVEGYFTIVLN
                     TNPYTYLGNRPLDIAPSATLDNGLVVVTFRSLAVTAVLRGLSGALRGGGVKPSATLDV
                     QTDVEALRITASEPFPYQLDGDYLGELTSLDFRHVPAAVRLVRPVLAPG"
     gene            complement(2493..>3836)
                     /locus_tag="PLV68_11770"
     CDS             complement(2493..>3836)
                     /locus_tag="PLV68_11770"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HRE01969.1"
                     /translation="PPYRVAPARAVAPVEPVWTPPQSWSPLQHPPDGPLPPVPGAAGF
                     DTAAAVGAGPKRRRLRWSAVAVLVLAALIGSAVTVVITRWVGDDDSATAGQPGGVIEL
                     RGHRDTVYLAGFSPDGATAFTSGADGRLIVWDARTGAEINRYEDLGAYIAAASFDRSG
                     STIVAAVDSQLVAIDAIAPGTRPAKVRLDLDLVHVVSTPDDDVIVVGFDPDDYEVGEI
                     LRFASVGGERLRRIGSGYPEALAPDGELALVSEYDRTGSTVTHVVDTLTGAETELEQL
                     LEFVFYADFAVFSDDGSKVARILFEYEDEPIALTGIWDTTTGALITELAVPADDHLML
                     AFDHSGQRLLVVSYGSDFFTTRLELFDANTGERQRTVTSQAVGGFSFSPDDAQIAVAT
                     ADGTVTLYDTASGHKQRVLEGHAGPIAAIAYSPDGSRLITASNDGTARIWHLTDN"
BASE COUNT          590 a         1292 c         1314 g          605 t
ORIGIN      
        1 ttggggcccg acgactcctc gacgtggacc gcggtgacct tggcgccacc agcggcaccc
       61 aggtcgccca ccggtgcgga gacgacctcg ggggcaccgc caccggcgga ctcggccgcg
      121 aagctcttcg gacggaacgc ggcgaggaac gggcccgacc cggtgaaggt ggtagtcacc
      181 agcgtgttgc cgccgaagat cggcgtggtg acggcgaagc tgtcgcccgc ggcctcgatg
      241 ttgacgttgt tggtcaacac cgtcttgtcg agcttgaccg acaggcggga cacgacgtcg
      301 cggccttcgg tgttctgcgg gaacaggatc agcgccgggg aatcgccccc gctgatgacg
      361 gcctgcatcg ccgaggcgac gacgacgccc ggcaggttgc cgccgnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn gcgcaccgcc ttgcgtccgg acgagctgct cacggcggtg
      481 accgtgccga ggctgcgggc cttggtgagc agttcgagcg tgccggtggt gggcgcgccg
      541 tctgcggtct gtgcaaacac ccagatgttg ctcatgatgg tcctcagatc actttcaggt
      601 tttcgagata ggcgacgatc tggccgaacg cttcgccatc gtcttcgacg atcgtgccgg
      661 ccgcgcgggc ctcggcctgg gcgacctcgg tgatcgactg gccggcaccg gcccagccga
      721 ccggagtgac gccgagatcg gcagcggtga cggtgtcgat cggcttcgac ttggcggcca
      781 tgatgccctt gaagctcggg tagcggggct cgaccacgcc ggcggtcacg ctgaccacgg
      841 cgggcagcga gcacgtgaca tcgtcgtagc cggcctcggt ctggcgctga gccgacaggg
      901 tgccgtcggc gatggccagc gacttggcga aggtgaccga cggcaggccg agcacctcgg
      961 cgatctgttc ggggacggta ccggtgtagc cgtcgctgga ctcggtgcca ccgatgacca
     1021 gatcgaagcc gccggcgcgc tggatggcag cagcgagcac cttggcggtg gtcagcgcat
     1081 cggaaccctg cagggccggg tcactgacga gcacgccctt ggcggcaccc atcgccagcg
     1141 cggtgcgcag cccggccgtc tcgccgttgg gagccatgga gacgagaatg acctcgccgc
     1201 cgccggcctt gtcgaccaac tggagcgcca tctccacgcc atagctgtcg gactcgtcga
     1261 ggatgagctt gccatcccgc ttcagagtgt tggtggaggg atccagcgcg cccggagcag
     1321 ccgggtctgg gatctgcttg acgcacacga tgacgttcat gtgcgccatt gttaccgacg
     1381 ggtcaatctg gcgccaactg ctgggcagag ttcacgctgg tacggtctgg tgcttgcgaa
     1441 tccggctcgt cgtcaacgcc ttcgcgtcgt ccgtcacggc gcgcaacacg gtggtcgtgc
     1501 accaggtgct gtcgcgcggc aacgacgtcg acgtggtcga gaccaaccgc cgcggccacg
     1561 ccacgcggtt cagccaggac gcggctcgac agggattcga cgtggtgatc ggctacggcg
     1621 gcgacggcac cctgaacgag gtcgccaccg gggtcgcggg caccaacacc gcgcttggtg
     1681 tgttgcctgg cggcagcacc aacgtgttcg cccgcaccct cggtatgccc aacgatccgg
     1741 tcgaagcggt gcacctgctg gccgcgggca tcgcccgcaa cgacatcgaa ccgatcggtc
     1801 tcggccacgt caacggccgc tacttctgct tccacaccgg cgtcggcttc gacgcggccg
     1861 tggtcaacca cgtcgagcgc cacggctcgc tgaagcggtg ggccgggcac cccttgttca
     1921 tctacgcggc gatgcgcacg atggccaact acgaccgcac tcagccccac ttcgaggtga
     1981 tcacgtccga ttccctcgac agttcgatct cgcgggtttc cacgtccgac gccgcgacgg
     2041 gacgcgaccg cgacccggtc gagggttact tcacgatcgt gctcaacacc aacccgtaca
     2101 cctatctggg caaccgcccg ctcgacatcg ctccctcggc caccctcgac aacggactgg
     2161 tcgtggtcac gttccggtcc ttggcagtga ccgccgtgtt gcgcgggctg agcggtgcgc
     2221 tgcgcggcgg cggggtgaag ccgtccgcca cgctggacgt gcagaccgac gtcgaagcac
     2281 tgcgcatcac cgccagcgag ccgttcccct atcaactcga cggcgactat ctgggcgaac
     2341 tgacctcgct cgacttccgc cacgtgcccg ccgctgtccg cctggttcgt ccggtgctcg
     2401 cgccgggctg atcgtctgca tgcgtccgtt atctacatgc atccgtcacc tacatgcgtc
     2461 aggtgccttc atgcgtcaat tgcctacaga cgtcagttat cggtcaggtg ccagatgcgg
     2521 gccgtgccgt cgttgctggc ggtgatcagc cgcgagccat cggggctgta ggcgatggcg
     2581 gcgatcggcc cggcgtggcc ttcgagcacc cgctgcttgt gccccgacgc cgtgtcgtag
     2641 agcgtgacgg tgccgtcggc ggtggcgacc gcgatctggg cgtcgtcggg actgaagctg
     2701 aacccgccca ccgcctggct ggtcacggtc cgctggcgct cgccggtgtt ggcgtcgaac
     2761 agttcgagcc gagtggtgaa gaagtcggaa ccgtacgaca cgaccagcag ccgctggccg
     2821 gagtggtcga aggcgagcat caggtggtcg tcggcgggca cagccaactc ggtgatcagc
     2881 gcgcccgtgg tggtgtccca gatcccggtg agcgcgatcg gctcgtcctc gtactcgaac
     2941 aggatgcgag ccaccttgct gccgtcgtcg gagaacacgg cgaagtcggc gtagaagacg
     3001 aactcgagca gttgttcgag ctcggtctcg gcgccggtca gcgtgtcgac gacatgggtg
     3061 acggtggaac cggtgcggtc gtactcggat accagagcca gttcgccgtc tggcgcgagc
     3121 gcctcgggat agccgctccc gatgcggcgc agtcgctcgc caccgaccga cgcgaagcga
     3181 agtatctcac ccacctcgta gtcgtcgggg tcgaagccga ccacgatcac gtcgtcgtcg
     3241 ggcgtggaga cgacgtgcac cagatcgagg tcgagacgga ccttggccgg tcgggtaccg
     3301 ggcgcgatgg catcgatcgc gaccagttgc gagtcgacag cggcgacgat ggtcgaaccg
     3361 gagcggtcga agctggccgc ggcgatgtac gcgcccaggt cctcgtagcg gttgatctcg
     3421 gcgcccgtgc gggcgtccca gacgatcaac cggccgtccg cgccggacgt gaaggccgtc
     3481 gccccgtccg ggctgaagcc cgccagatac acggtgtcac gatgaccgcg cagttcgatc
     3541 acgccacccg gttggcccgc tgtggccgag tcgtcgtcgc cgacccagcg cgtgatcacc
     3601 acggtgaccg ccgaaccgat cagcgcggcc agcaccagca cagcgacggc cgaccaacgc
     3661 agccgtcgcc gcttcgggcc ggcgcccacc gcagcggccg tatcgaaccc agccgccccc
     3721 ggcaccggag gcagcgggcc gtcgggaggg tgttgcagcg gtgaccacga ctgcggcggc
     3781 gtccacaccg gctcgaccgg agccacggcc ctggcgggcg cgacccgata cggcgg
//
LOCUS       DAORNF010000286         3813 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_18024_length_3813_cov_1.381586, whole genome shotgun sequence.
ACCESSION   DAORNF010000286 DAORNF010000000
VERSION     DAORNF010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3813
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18024_length_3813_cov_1.381586"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            94..>346
                     /locus_tag="PLV68_11775"
     CDS             94..>346
                     /locus_tag="PLV68_11775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01970.1"
                     /translation="MVGPSSRRPLRVVACGVVALGLLASCRSGESGGANVPVSELTLV
                     PDSIELGPDNCDPADQITPPRITFGGQTIDIRSPGLFISE"
     assembly_gap    347..356
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <357..>740
                     /locus_tag="PLV68_11780"
     CDS             <357..>740
                     /locus_tag="PLV68_11780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01971.1"
                     /translation="LGLSDVGCAGVNGDGYISFDYDPALLDEPVGGNGSLTVVIATGD
                     TATMELSGTQLSDTEQPVETTAGRWTVTVPAQSCVRLTIDLVSANGSGSATYGVDLRR
                     GGEAVECDQRDLPPEEAPELDPDAPP"
     assembly_gap    741..849
                     /estimated_length=109
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1096..1248
                     /estimated_length=153
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1249..>1780)
                     /gene="rsgA"
                     /locus_tag="PLV68_11785"
     CDS             complement(<1249..>1780)
                     /gene="rsgA"
                     /locus_tag="PLV68_11785"
                     /inference="COORDINATES: protein motif:HMM:NF015170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTPase RsgA"
                     /protein_id="HRE01972.1"
                     /translation="EHVLPRQSAFVRRASFEGNRAVAHTVAANIDVVCLVHALNSPPN
                     QRRLERELVLAFDSGARPVVVLTKLDLVDADEIGRSVAALEEVALGVAVYAASGTTGE
                     GVDTLRGFALDGKTLAFLGASGVGKSTLINALVGSDERATAVVRAGDQRGRHTTTAAE
                     LVPLPNAGARPGGWLLD"
     assembly_gap    1781..1791
                     /estimated_length=11
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1792..2033)
                     /locus_tag="PLV68_11790"
     CDS             complement(<1792..2033)
                     /locus_tag="PLV68_11790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01973.1"
                     /translation="MTDDPARPRPDRASPSLSPSPPASMGWSDWFEQDWDERGRVGTP
                     ARVVRLDRGWSSLLTELGAGVQRVRNIGADVAVGDWV"
     gene            2203..>3421
                     /gene="topA"
                     /locus_tag="PLV68_11795"
     CDS             2203..>3421
                     /gene="topA"
                     /locus_tag="PLV68_11795"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HRE01974.1"
                     /translation="MARPLVIVESPAKAKTISKFLGSTFDVRASVGHVADLPSKGLAV
                     DVDNGFKPTYELTERGKTVVKDLRALLKDASELYLATDEDREGEAISWHLLEYLKPKV
                     PVKRMVFHEITKTAIDHAVANPRSIDYGLVDAAETRRILDRLYGYEVSPVLWRRVNRG
                     LSAGRVQSPTIRLVVERERERIAFVVAGYWDLQLATATSPAFDATLVGLDRVKVASGK
                     DFSSTGIAKADVVVLDQAQAHTLADGLAGATVVVRSVEEKPYRSSPKAPFMTSTLQQE
                     GGRKLRLSAAQVMRVAQGLYERGFITYMRTDSVALSAEALTAVRAEVARTYGDTYLSG
                     APRQFTSKSRNAQEAHEAIRPTTPLRAPDQVAAELNSQELGLYRMIWQRTLASQMADA
                     AGITGSVRLGATAG"
     assembly_gap    3422..3600
                     /estimated_length=179
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3601..>3813
                     /locus_tag="PLV68_11800"
     CDS             <3601..>3813
                     /locus_tag="PLV68_11800"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HRE01975.1"
                     /translation="PNGHTTSPPARYTEASIVKRLEELGIGRPSTWASIIQTVQDRGY
                     VWKKGQALVPTWTAFAVVGLLEQHFG"
BASE COUNT          558 a         1201 c         1089 g          503 t
ORIGIN      
        1 gccttaccca gatcaccggc ttcgaccgcg ccgtcgcggt tgccgcatcc ctcgccgacg
       61 cgttcggctg actcgcggct acggtcgccg tcggtggttg gtccttcgtc ccgtcgtccg
      121 ctgcgcgtcg tcgcgtgcgg tgtggtcgcg ctcgggctgc tcgcatcgtg ccgcagcggc
      181 gagtcaggtg gggccaacgt gccggtcagc gagctgacac tggtgcccga ttcgatcgag
      241 ctcgggcccg acaactgcga cccggccgac cagatcaccc cgccgcggat caccttcggc
      301 ggccagacga tcgacattcg gagtccgggc ctcttcattt ccgaagnnnn nnnnnngctc
      361 ggcttgtccg acgtcggttg cgcgggggtc aacggcgacg ggtacatctc gttcgactac
      421 gacccggcac tgctcgacga gccagtcggc ggcaacggtt cgttgaccgt tgtcatcgct
      481 actggcgaca ccgcaaccat ggagttgtcg ggtacgcagt tgtccgacac tgaacagccc
      541 gtcgagacca ccgccggacg ctggacggtg accgtcccag cccagtcgtg cgtacgtctc
      601 acgatcgatc tggtcagtgc gaacgggtcg ggctcggcca cctatggcgt cgacctccgt
      661 cgcggcggcg aagcggtcga gtgcgatcag cgcgacctgc ccccggaaga agctcccgaa
      721 ctggaccctg acgcgccacc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      841 nnnnnnnnnt caccaccatc ccctgatcag ccgagcgcct ggcaccagcc ggtcacattc
      901 ccatcgagtc gagcggcacc gcggtatgcg catcgctccc cggatcagcc gaagcggttc
      961 cggcgggggc ggtggacacc gcggcgatcg gcggccttct cctgggcgcg ctgttcgtct
     1021 tcgagcgccg cttcttcggc caccagacgc tgcaggctgg caaggcggct gaccgccagg
     1081 tcgccgtccg cgatcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngt cgagcaacca
     1261 tccacccgga cgcgccccgg cattgggcag cggcaccaac tctgccgccg tggtggtgtg
     1321 acgaccgcgc tgatcaccgg cgcgcaccac cgcggtggcc cgctcgtcgc tgcccaccag
     1381 cgcgttgatc agcgtcgact tgccgacacc gctggcgccg aggaacgcca gcgtcttgcc
     1441 gtcgagggcg aagccgcgca acgtatcgac cccctcaccc gtggtgccgc tggccgcata
     1501 gaccgccacc ccgagcgcca cctcttccag cgcggcgacc gagcggccga tctcgtcggc
     1561 gtcgaccaga tcgagcttgg tcagcactac caccggccgg gcaccgctat cgaaggccag
     1621 caccaactca cgctccaagc ggcgttggtt gggagggctg ttgagcgcgt gcaccaggca
     1681 gaccacgtcg atgttcgcgg cgacggtgtg ggcgacggca cggttgcctt cgaacgaggc
     1741 gcgacggacg aacgcgctct ggcggggcaa cacgtgctcg nnnnnnnnnn nacccagtcg
     1801 ccgaccgcaa cgtcggcgcc gatgttgcgc acccgttgca cgccggcgcc gagttcggtc
     1861 agcagactgc tccagccgcg gtcgaggcgc acgacccgcg ccggagtgcc cacgcggccg
     1921 cgctcgtccc agtcctgttc gaaccaatcg ctccatccca tcgatgctgg gggagacggc
     1981 gagagagacg gcgacgcacg atcggggcgc ggacgtgccg gatcgtcggt catgcgcgaa
     2041 cgtctctcca cagcatcgtg aacatcatcg tgaacatgac ggtaaggact ggtgagcagc
     2101 gcgtcaacgt tgccgaccgt atgctgcccg ctgtcagcag cccagccacc tcggcaaggc
     2161 gtttgacaag caccccctcg atcccgtaac gaataccgca ccatggcacg accactcgtc
     2221 atcgtggagt cgccggcgaa ggccaagacg atctccaaat ttctcggcag cacgttcgac
     2281 gtgcgcgcct cggtgggcca cgtggccgac ctcccgagca agggtctggc cgtcgatgtc
     2341 gacaacgggt tcaaacccac ctacgaactc accgagcgcg gtaagaccgt cgtcaaagat
     2401 ctgcgcgccc tcttgaagga tgcgagcgag ctgtatctcg ccaccgacga agatcgcgaa
     2461 ggtgaagcga tcagctggca cctgctcgag tacctgaagc cgaaggtgcc ggtgaagcgg
     2521 atggtgttcc acgagatcac caagaccgcc atcgaccacg cggtcgcgaa cccgcgctcg
     2581 atcgactacg gcttggtcga tgcggccgag acacgccgca tcctcgaccg gctctacggc
     2641 tacgaggtca gccccgtgct gtggcgccgc gtcaaccgcg gcctgtcagc cgggcgggtg
     2701 cagagcccca cgatccgcct ggtggtcgaa cgcgaacgcg aacggatcgc gttcgtcgtc
     2761 gcgggctact gggatctgca actggccacc gccaccagcc cggcgttcga cgcgacgctg
     2821 gtgggcctcg accgggtcaa ggtcgcctcc ggcaaggact tctccagcac cggcatcgcc
     2881 aaggccgatg tggtggtgct cgatcaggcc caggcccaca cgctggccga cgggctcgct
     2941 ggcgccaccg tcgtcgtccg ctcggtcgag gagaagccct atcgcagcag ccccaaggct
     3001 ccgttcatga ccagcacgct gcagcaagag ggcggccgca agctccgcct cagcgcggcc
     3061 caggtcatgc gcgtcgccca gggtctgtac gagcgggggt tcatcaccta catgcgaacc
     3121 gacagcgtcg cgttgtcggc cgaggctctg accgccgtgc gggccgaggt cgcccgcacc
     3181 tatggcgaca cctatctgtc gggcgcgccg cgccagttca ccagcaagag ccgcaacgcc
     3241 caagaagcgc acgaggcgat ccgccccacc acgccgttgc gggcacccga tcaggtggcg
     3301 gccgaactga actcgcaaga gctgggtctg taccgcatga tctggcagcg cacgttggcg
     3361 tcgcagatgg ccgacgcggc gggcatcacc ggcagcgtcc gcctcggcgc caccgccggg
     3421 cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 cccccaacgg ccacaccaca tcgcccccgg cgcgctacac cgaggccagc atcgtcaagc
     3661 ggctggaaga gttgggtatc gggcgcccga gcacgtgggc cagcatcatc cagaccgtgc
     3721 aagaccgcgg gtacgtgtgg aagaagggtc aagccctggt gcccacgtgg accgcgttcg
     3781 ccgtcgtcgg gctgctcgaa cagcacttcg gtg
//
LOCUS       DAORNF010000287         3798 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_18120_length_3798_cov_1.619022, whole genome shotgun sequence.
ACCESSION   DAORNF010000287 DAORNF010000000
VERSION     DAORNF010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3798
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18120_length_3798_cov_1.619022"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>550
                     /locus_tag="PLV68_11805"
     CDS             <1..>550
                     /locus_tag="PLV68_11805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01976.1"
                     /translation="YLVITLAIEPRFALWNTHILSESLGMTLAVVSVVCWVRFAHNPA
                     ASTVRWAALATIGWMLTRDSNVIPALAATVPAMLAASWWWRSAGERLRSSLRVWAVVL
                     IAVGLVVSVAQADSGRNRYPTMNNVGLRVLPNPGLTAWFAGHGMPVDATLLGRAGHSS
                     FDDGFAMLTWPELAEFRRWADTS"
     assembly_gap    551..764
                     /estimated_length=214
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <765..1032
                     /locus_tag="PLV68_11810"
     CDS             <765..1032
                     /locus_tag="PLV68_11810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01977.1"
                     /translation="AALGRRRVMAVVLALGLASAFVDLYLAYVGDSVEVLRHTVGPQA
                     RLSLLMVVSVGVGADTVLAVIGGRRRTVTSTGDVSDENRPGAAT"
     gene            1059..1334
                     /locus_tag="PLV68_11815"
     CDS             1059..1334
                     /locus_tag="PLV68_11815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01978.1"
                     /translation="MVLLGAVFMVGAFVLAALFGNEHRSQDWDPMYARDVVLRSMRFG
                     GTFVENGLVNKGPLEAAVYRLAASLSSYDGYWFVISAFVLIAAALIG"
     gene            1376..3271
                     /locus_tag="PLV68_11820"
     CDS             1376..3271
                     /locus_tag="PLV68_11820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01979.1"
                     /translation="MAIGIVVLFHFTISKADYAGVLYSRNMIVGLFAATWLIALGRRW
                     WTPRRAPWAALAVGVLVGLAIQTLFVSSIAALGVWLLAWSSTDVLDDETAWRRSRRTL
                     VITPVVVFATAPLYYAVRGVFAEFWDGYWIYNTFQNAATGRTLAGQLVFGRDAILRYY
                     RAWPASLLIVVAFVVLTAALWAGFDRRTRCLHVAVAVWFVGAWAELITGQRYSSHYFS
                     VLAVPTAMMAALVAGHLYRLLRTARGEREFRSVVAWPLVASLLAIAAWGGKGLTTGLQ
                     EASRFRSVSDLAAQRRAAEPGQQRTVRAVLDVFSTADDPLLAWTDYPWVYLNYHRTSA
                     TRFVWKTFLVGEVYLGRSSADYVLDRTWGWFAHDMAQTNPGVFLAETALPVDGETPFA
                     AYVAANFQPVFADEQNVVYARNDLAAAMAAGATGGATLTPRAVAVGTPWLTGAGWATR
                     AGDAPADAIDALVLSDQRCVQVSGMLAADPERHFPLDGEGAGGQSPHDATRDDASAGD
                     ATGADGAANPNGGSYISFHVEDPAGRGADLQMSITPDRAISGDAGTVFDSIPLPSVEQ
                     DPTNTDAGAQPFTIVVGERAAVLVIGGQIRAAVRLDGQSRLSVEVREGGVRLYDLRLA
                     PAPPSAC"
     assembly_gap    3349..3460
                     /estimated_length=112
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3476..>3798
                     /locus_tag="PLV68_11825"
     CDS             3476..>3798
                     /locus_tag="PLV68_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HRE01980.1"
                     /translation="MLGADDVRRAITRIAHEIIERNRGLDGVVLIGLQRGGVWIAERL
                     AAAIAAIEPGAAVPVGRVDVSMYRDDIGLRPISPASVTEIPVALDDAAVVLVDDVLYT
                     GRTVRA"
BASE COUNT          444 a         1161 c         1275 g          592 t
ORIGIN      
        1 tacctggtga tcacgctcgc gatcgagccg cggttcgcgc tgtggaacac gcacatcctc
       61 agtgagtcgc taggtatgac gctcgcggtc gtgtcggtgg tgtgctgggt gcggttcgct
      121 cacaatcccg cggcatcgac agtgcgctgg gccgcgctgg cgacgatcgg ttggatgctg
      181 acccgcgact ccaacgtgat ccccgcgctg gccgcgaccg ttcccgcgat gctggccgcg
      241 tcatggtggt ggcgttcggc gggagagcgg ttgcgctcgt cgctgcgggt atgggcggtg
      301 gtgctgatcg ccgtcggtct ggtcgtgtcg gtggcccagg ccgacagtgg gcgcaatcgc
      361 tacccgacga tgaacaacgt cggtctgcgg gtgctgccca accccggcct gaccgcgtgg
      421 ttcgccgggc acggcatgcc ggtggacgcg acgttgctcg gtcgcgctgg gcacagcagc
      481 ttcgacgacg ggttcgcgat gttgacgtgg cccgaactgg ccgagttccg ccgctgggcc
      541 gacaccagcg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncgccgc gctcgggcgg
      781 cgacgggtga tggccgtcgt gctcgcgttg gggttggcct cggccttcgt cgatctgtac
      841 ctggcctacg tcggagactc ggtggaggtc ttgcgccaca ccgtcgggcc acaggcacgc
      901 ctgtcgttgt tgatggtggt gagcgtcggt gtcggtgccg acaccgtgct ggccgtgatc
      961 ggcggccgtc gtcggaccgt cacgtcaacc ggtgacgtat ctgacgagaa ccgaccgggg
     1021 gcggcgacgt gaccacgggt cggtcgcggt gggggacctt ggtgctgctg ggcgcggtgt
     1081 tcatggtggg cgcgttcgtg ctcgccgcgc tgttcggcaa cgagcatcgc agccaggact
     1141 gggacccgat gtatgcccgc gacgtcgtgc tccgctcgat gcgcttcggc ggcaccttcg
     1201 tggagaacgg cctggtcaac aagggtccgc tggaggccgc ggtctatcgg ctggcggcct
     1261 cgttgagctc gtacgacggc tactggttcg tgatctcggc cttcgtcttg atcgctgcgg
     1321 cactgatcgg ctgagccgca ccacccaggc ggtcggtggc caccgcctgc tcggcgtggc
     1381 gatcggcatc gtcgtgctgt tccacttcac gatcagcaag gccgactacg ccggggtgct
     1441 gtacagccgc aacatgatcg tcgggctgtt cgccgccacc tggctgatcg cgctgggccg
     1501 acgatggtgg acgccgaggc gagccccgtg ggccgcgctg gccgtcggtg tgttggtcgg
     1561 gttggcgatc cagacgctgt tcgtgtcgag catcgccgcc ttgggcgtgt ggctgttggc
     1621 gtggtcgagc accgacgtgt tggacgacga gacagcctgg cgacgcagcc ggagaacgct
     1681 ggtgatcacc cccgtcgtgg tgttcgcgac cgccccgctg tactacgccg tgcgcggcgt
     1741 gttcgccgag ttctgggacg gttactggat ctacaacacc ttccagaatg cggccaccgg
     1801 gcgcacgctg gcgggccaac tggtgttcgg ccgcgacgcg atcctgcgtt actaccgggc
     1861 gtggccggcg tcgcttctga tcgtcgtcgc gttcgtggtg ctcacggccg cgctgtgggc
     1921 cggcttcgat cggcgtaccc gctgcctgca tgtcgcggtc gcggtgtggt tcgtcggcgc
     1981 gtgggccgag ctgatcaccg gccagcgcta ctcgtcgcac tacttctcgg tgctcgcggt
     2041 gccgaccgcg atgatggccg cgttggtggc cgggcatctg taccgcctgc tgcgcacggc
     2101 gcgcggtgag cgtgagttcc gctcggtcgt cgcctggccg ctggtggcgt cgttgttggc
     2161 gatcgcggcg tggggcggca aggggctgac gaccgggctg caagaagcga gccgcttccg
     2221 cagcgtgtcc gacctggccg cgcaacgccg ggccgccgag ccgggtcagc aacgcacggt
     2281 gcgggccgtg ctcgacgtgt tctccaccgc cgacgacccg ctgctggcgt ggaccgacta
     2341 cccgtgggtc tacctcaact accaccgcac ctccgcgacc cggttcgtct ggaagacgtt
     2401 cctcgtcggc gaggtctacc tcggccgatc cagcgccgac tacgtgctcg accggacgtg
     2461 gggatggttc gcccacgaca tggcccagac caacccgggg gtcttcctgg ccgagaccgc
     2521 gctgccagtc gacggggaga caccgttcgc ggcctacgtc gcggccaact tccaaccggt
     2581 gttcgccgac gagcagaacg tggtctacgc ccgcaacgac ctggccgcgg cgatggcggc
     2641 cggtgccact ggcggtgcga cgctgacgcc ccgcgcggtc gcagtcggca cgccttggct
     2701 gacgggtgcg ggatgggcga cacgcgctgg cgacgcaccg gccgatgcga tcgacgcgtt
     2761 ggtgctgtcc gaccaacgct gcgtccaggt cagcggcatg ttggccgccg accccgaacg
     2821 ccacttcccg ctcgacggcg aaggggcagg ggggcagtcg ccgcacgacg cgacccgtga
     2881 cgatgcgagc gctggtgacg cgacgggcgc agacggcgcc gccaatccca acggtgggtc
     2941 gtacatctcg ttccacgtgg aagatcccgc cggccggggc gcggacctgc agatgagcat
     3001 cacacccgat cgggcgatct cgggcgacgc gggaaccgtg ttcgacagca ttccgctgcc
     3061 cagcgtcgaa caggacccca cgaacaccga cgctggtgcg cagccgttca cgatcgtggt
     3121 cggtgagcgg gccgcagtgc tggtgatcgg cggtcagatc cgcgccgcgg tgcgcctcga
     3181 cggccagtcg cggctcagcg tcgaggtccg cgaaggaggc gtgcggctgt acgacctgcg
     3241 cctcgccccg gccccgccga gcgcctgctg agcgcctgct gagtgctgct gagaatcccg
     3301 cgcgggcggt atcggctgct agcggggcgc cgaccgtcaa acgggtccnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cccgccagca agctggtgct
     3481 cggtgccgac gacgtgcgcc gggcgatcac ccgcatcgcc cacgagatca tcgagcgcaa
     3541 ccgcggcctc gacggcgtgg tgctgatcgg gctccagcgc ggcggcgtgt ggatcgccga
     3601 gcgcctggcc gcggcgatcg ccgcgatcga accgggcgcg gcggtgccgg tcgggcgcgt
     3661 cgacgtgtcg atgtaccgcg acgacatcgg gctgcggccg atctcgcccg catcggtcac
     3721 cgagatcccg gtcgcactcg acgacgcagc ggtagtgctg gtcgacgacg tgttgtacac
     3781 cggccgcacc gtgcgcgc
//
LOCUS       DAORNF010000288         3790 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_18173_length_3790_cov_1.621419, whole genome shotgun sequence.
ACCESSION   DAORNF010000288 DAORNF010000000
VERSION     DAORNF010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3790
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18173_length_3790_cov_1.621419"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    258..381
                     /estimated_length=124
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <382..>786
                     /locus_tag="PLV68_11830"
     CDS             <382..>786
                     /locus_tag="PLV68_11830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01981.1"
                     /translation="GIVQLGARVPGNGPRDLAATALFGYELAPSGIPEPPARGEVYAD
                     NVLRADGVEVGMEILLGPAHSPVTVIGFVNDVSYSGQGSLWAEPGTWREVMAANRPDV
                     RLPDDSFQALVVQASDGVDADRLADDIDDAVGS"
     assembly_gap    787..819
                     /estimated_length=33
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <820..>1189
                     /locus_tag="PLV68_11835"
     CDS             <820..>1189
                     /locus_tag="PLV68_11835"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HRE01982.1"
                     /translation="PGVRQQRSTFNQIIGVTIAIAAVVVALFFALLTVERLALYGVLK
                     ALGARSRTLFAGLVAQAVVVTTAASIIAGGAAFAIDALIPAGSIPLDISPRRVFLSAA
                     LLLAAAIVGCGFSLRRVLRVD"
     assembly_gap    1190..1319
                     /estimated_length=130
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1334..1945
                     /locus_tag="PLV68_11840"
     CDS             1334..1945
                     /locus_tag="PLV68_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRE01983.1"
                     /translation="MALDHADLRVEPGEIVALVGPSGSGKTTLCSIAGGLLTPTGGTI
                     VVGGEDISDYSAKQLTEFRQHKVGFVFQSVNLVPFLTARENLLVVDELGRRTGAAARA
                     RADQLLDELGLGDRSKNLPAQLSGGQRQRVAIGRALMNDPELVLFDEPTSALDTKLGE
                     QVMELIRREMKDRGTAAVVVTHDDRMTHHCDRVVHIVDGVIAA"
     assembly_gap    2360..2384
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2385..2865
                     /locus_tag="PLV68_11845"
     CDS             <2385..2865
                     /locus_tag="PLV68_11845"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase activity [Evidence
                     IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HRE01984.1"
                     /translation="VEASDGPPPGAVALRERLEAADGFVISTPEYNASMPGVLKNVID
                     WVSRARPQPFDGHRALLLSASPSMVGGNRSLWSLRVPLENLGVHVYPGMFSLARAHTA
                     FDEEGRIADVNLQQRFDDTVAAFLDLVEASTHYTCVKRAWVEFLGETPDPAVDRGEP"
     assembly_gap    3041..3050
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3489..3579
                     /estimated_length=91
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3580..>3790
                     /locus_tag="PLV68_11850"
     CDS             <3580..>3790
                     /locus_tag="PLV68_11850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01985.1"
                     /translation="EMAATHFYHRHYFQAYGVADRELNARPAEFLAKVFWALSGDQRY
                     LDVWGHPSEGNGYLDVLPEPPPLPWD"
BASE COUNT          498 a         1125 c         1172 g          582 t
ORIGIN      
        1 gtcgtgctgg gatggtcggc gttgctggcg gcgctcggtc ttgcctcgtc gttgctcgcc
       61 gcccgacgcg tgctggccat cgatcccgtc gcggcgacaa gcggggcagg ggtggggcga
      121 tgaagctcgc gctgcgtgag ctccgtcgtc gaccgggacg attcgtcacg gcggcgatca
      181 ttctgacgct cgtcgccgtg ttggtgatgt tcctcggcgg tctgctcgac ggcctcatcc
      241 gaggctcgac gggcgccnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nggcatcgtg caactcgggg cgcgggtgcc gggcaacggt
      421 ccacgtgatc tggctgcgac agcactgttc ggctacgagt tggcgccgtc cggcatcccc
      481 gagccaccgg cccgagggga ggtctacgcc gacaacgtcc tgcgcgccga cggggtagag
      541 gtcggaatgg agatcctgct cggcccagcc cattcgccgg tgaccgtgat cgggttcgtc
      601 aacgacgtga gctactccgg acaggggagc ctgtgggctg agccggggac gtggcgagag
      661 gtgatggcgg ccaaccgacc cgacgtccgc ctgcccgacg actcgttcca agcgctcgtg
      721 gtgcaagcct ccgacggcgt cgatgccgac cgactcgccg acgacatcga cgacgccgtc
      781 gggagtnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc cgggtgtgcg tcaacagcgc
      841 tcgacgttca accagatcat cggcgtgacg atcgcgatcg cggcagtagt ggtggcgttg
      901 ttcttcgcgc tgctcaccgt cgaacgcctc gctctctacg gcgtgctcaa agcgctcggg
      961 gcgcgcagtc gcacgctctt cgccgggctc gtcgcccaag ccgtcgtcgt caccacggcg
     1021 gcgtcgatca tcgccggcgg cgccgcgttc gcgatcgacg cactcatccc ggccggttcg
     1081 attccactcg acatctcgcc gcgccgggtg ttcctcagtg ccgccctcct tctcgccgcc
     1141 gccatcgtcg gctgcggctt ctccctccgc cgggtactcc gcgtcgaccn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc
     1321 cgacgaggag gtcgtggccc tcgatcacgc cgacctccgg gtcgagccgg gcgagatcgt
     1381 cgccctggtc ggtccgtccg gttcaggcaa gacgaccttg tgctcgatcg ccggcggact
     1441 cctcaccccc accgggggca ccatcgtcgt cggtggcgag gacatctctg actactcggc
     1501 caagcagttg accgagttcc gccagcacaa ggtgggcttc gtgttccagt ccgtcaatct
     1561 cgtgccgttc ctgacggcac gcgagaacct cctcgtggtc gacgagctcg ggcgtcgcac
     1621 cggtgccgcc gcgcgcgcac gcgccgatca gctgctcgac gagctcggcc ttggtgaccg
     1681 ctcgaagaac ctccccgctc agttgtccgg cggacagcgg caacgtgtcg cgatcggcag
     1741 ggcgttgatg aacgatcccg agttggtgct gttcgacgag ccgaccagtg cgctcgatac
     1801 caagctcggc gagcaggtga tggagttgat ccgtcgtgag atgaaggacc gcggcacggc
     1861 agccgtcgtg gtcacccacg acgatcggat gacacatcac tgcgaccgtg tggtgcacat
     1921 cgtcgacggg gtgatcgccg catgaaaggg ctcgcgagga gctcgtagaa tcgcgaggga
     1981 cgcaggcgcc agaggccagg cccgaacgac gtagaccgcg gccgtctggt gaagcaggaa
     2041 cggttccggg gtcctcgtcg gccagtctcc gggcgcaacg ggctgtccgg ccgcgatccg
     2101 gagagcggcg tgacaaccag aaatgggctg atgacgaaaa ggtcgcaggt tcccaatcct
     2161 gccccgccac tcaggaaatc cctggtcgat cgcgcaggtg cgaagatgtc atggtgtcca
     2221 atgagcgtga accactgaag atcctggtgt tttcggcgtc gctgcgcgca gggtcgctca
     2281 atacgcggct cgcgcgtctc gccgcggatg ctgccgagcg tcacggcgcg acggtggatc
     2341 tggcgtcgat gcgcgagttn nnnnnnnnnn nnnnnnnnnn nnnncgtcga agccagcgac
     2401 gggccaccgc ctggagcggt ggcgttgcgg gagcggttgg aggcggcgga cgggttcgtg
     2461 atctcgacgc cggagtacaa cgcgtcgatg ccgggtgtgt tgaagaacgt catcgattgg
     2521 gtgtcgcgag cgcgcccgca gccgtttgat ggacatcgcg cgctgttgct gtccgcgtcg
     2581 ccgtcgatgg tcggcggcaa ccggtcgctg tggtcgttgc gggtgccact cgagaatctt
     2641 ggcgtccatg tctatccggg catgttctct ctcgcccggg cgcacaccgc gttcgacgag
     2701 gagggacgca tcgccgacgt gaatcttcaa cagcggttcg acgacacggt cgctgccttc
     2761 ttggatctgg tcgaggcatc gacccactac acgtgcgtca agcgggcgtg ggtcgagttc
     2821 ctcggggaga cgcccgatcc tgcggtcgat cgcggcgagc cctgaccatc gcgagccgac
     2881 cagagcggcc gcaccgaagc cgtccacaac aaatccacgg agtcgatcgg gcgcggcgcg
     2941 ccggtcggtc ccactcaccg ggcggcggga gttgagcgaa agccgccgat ccggctggaa
     3001 tcgacctacc gactctcggc gctgcacgag cgacggggac nnnnnnnnnn aggtaggccg
     3061 tggtcaactc gccaccaggg tatgggacgt ggttgtcgcc ggcgtgctcg tcgccgcggt
     3121 ggctcgcccg atgagcgccg tcgacatcga tgtcgcgctg cggcaccgct ggatcgagac
     3181 gaacggcgtc cgactgcacg ctgtcgaggc tggcaccggt acccctgtga tcttcttgca
     3241 tgggtttcct ggcctgtggt actcgtggca ccgccagctg cccgtgattg cggccgccgg
     3301 gtggcgagcc gtcgccatcg atcagctcgg actcggtcgc tcggaccggc cgcaccagcc
     3361 cgtgcgctac tcccatgacc acgtcatcgc cgacgtcgtc gggctgatcg acgcgctcgg
     3421 agccgagcgg gccgtgatcg tcggccatga cttcggcgct ccgctggcgt ggaatgtggc
     3481 ggtgcgcgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng agatggccgc aacacacttc
     3601 taccatcgcc actacttcca ggcctacggc gtcgccgacc gggagctgaa cgcccgcccc
     3661 gccgagttcc tcgcgaaggt cttctgggcc ttgagcggcg accagcgcta cctggacgtc
     3721 tgggggcatc cgagcgaggg caacggctac ctcgacgtgc tgccggagcc gccgccgctg
     3781 ccgtgggact
//
LOCUS       DAORNF010000289         3722 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_18613_length_3722_cov_1.727025, whole genome shotgun sequence.
ACCESSION   DAORNF010000289 DAORNF010000000
VERSION     DAORNF010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3722
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18613_length_3722_cov_1.727025"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="PLV68_11855"
     CDS             <1..298
                     /locus_tag="PLV68_11855"
                     /inference="COORDINATES: protein motif:HMM:NF025819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HRE01986.1"
                     /translation="APRDLLRTEIAAEDEGLAVIGVVHSHTHTEAYPSPTDVAQAPDP
                     TWHYVIVSLKHDQPQVRSFRIVDGEITEEPITITDEPIATTAAATTATTTDGCC"
     gene            430..1386
                     /locus_tag="PLV68_11860"
     CDS             430..1386
                     /locus_tag="PLV68_11860"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HRE01987.1"
                     /translation="MTNVLDLIGNTPMVDVSVLSPNPRVRIYAKLEGQNPFGSVKDRI
                     AKYMIEEAEKNGTLHPGQTIMEPSSGNTGIAMAAIARLKGYPITILMPDSVSVERRQM
                     LEVFGAEIVLTPGGEGSNGAVARARALADEHPEYCFLYQYANEANPRAHYEGTGPEIW
                     RDLPEITHFVAGLGTSGTLMGTGRYLKEQNPDIKVIAIEPPLGERVEGLRNLDDGYIP
                     PLFETWNGYELLDRKRVVRPRESLEWARRLVSECGLFVGLSAGAAMAGAAKVAAEVDA
                     SEADDTVIAFIVADGGWKYLSTGAYTDDLDVAEARAEEIIYF"
     gene            complement(1377..2171)
                     /locus_tag="PLV68_11865"
     CDS             complement(1377..2171)
                     /locus_tag="PLV68_11865"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HRE01988.1"
                     /translation="MTSLADLRARIDELDRQLIDVLAQRLAVCEEVARVKEGTDTPVI
                     QPARVRDVVTSRRQWAIDSGVDPDFAEQVMRVLLAETHRIEVAGARTDGKPDKLAAPD
                     TARSAIDTVVTRLDHVVIVVDDLGAAAATFTDRFGFHPVALADGEHPGIQAVQAGGAV
                     IVLVGPEAGDAVAAHLAAHGSGIQHVSVDVLNADYARAALTAADAPLLTEIVVDDDGH
                     EEFFTTRDAAIGFQLGFISRTGHRVGIGGANILASFAAPDEASDQK"
     gene            2341..2595
                     /gene="rpsO"
                     /locus_tag="PLV68_11870"
     CDS             2341..2595
                     /gene="rpsO"
                     /locus_tag="PLV68_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HRE01989.1"
                     /translation="MDKLDTIAEHRLHDTDTGSPEVQIALLTQRINHLTEHLKFHKKD
                     HHSRRGLLMLVGQRRRLLDYVRARDIERYRAIIARLGLRR"
     gene            2974..>3722
                     /locus_tag="PLV68_11875"
     CDS             2974..>3722
                     /locus_tag="PLV68_11875"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HRE01990.1"
                     /translation="MSGADGSRHEGRNGGLAYLPGLDGLRALAVAAVVAFHFGAGWAT
                     GGFLGVSLFFTLSGFLITRVLLAERSADGHVRLRGFWGRRIRRLTPAAMVCLAGVLVV
                     MAVRGDAGHARQVGHDVLASAFNVANWHFVSSSVSYGDLFSGWRSPVLHFWSLAIEEQ
                     FYVLFPVLFVVVARWHDRLDRRGLDRRGLDRRGRRWRSAPAVVAVTVVGAILASVVAS
                     WLTADGDLVYFGTHTRAGELLIGVLLALAVEA"
BASE COUNT          550 a         1235 c         1300 g          637 t
ORIGIN      
        1 cgcgccgcgc gacctgctgc gcaccgagat cgcggccgag gacgagggcc tggcggtgat
       61 cggcgtggtc cacagccaca cccataccga ggcctacccc agccccaccg acgtcgccca
      121 ggcgcccgac ccgacgtggc actacgtgat cgtcagcctc aagcacgacc agccgcaggt
      181 gcgtagcttc cgcatcgtcg acggcgagat caccgaagag ccgatcacga tcaccgacga
      241 gccgatcgcc accaccgccg ccgccaccac cgccaccacc actgacgggt gttgctgacg
      301 caccgacggg tgttgctggc ttgtcggcgt atccacatcg gtgaacaatc gatactattc
      361 cgattggact cgtcgggatt tcgtcgggcc acggcacgca acgtgacgag aggagccgcc
      421 atggtgccca tgaccaacgt gctcgatctc atcggcaaca cgccgatggt cgacgtcagc
      481 gtgctgtcac cgaacccgcg ggtgcgcatc tacgccaagt tggaggggca gaacccgttc
      541 ggctcggtca aggaccgcat tgccaagtac atgatcgaag aggccgagaa gaacggcacg
      601 ctccatccgg gccagacgat catggagccg tcgtcgggca acaccggcat cgcgatggcg
      661 gcgatcgccc gcctcaaggg gtacccgatc accattctga tgcccgacag cgtcagcgtc
      721 gaacggcgcc agatgctgga ggtgttcggg gctgagatcg tgttgacacc cggcggggag
      781 ggcagcaacg gtgccgtcgc ccgcgcccgt gcgctggccg atgagcaccc cgagtactgc
      841 ttcttgtacc agtacgccaa cgaggccaat ccccgtgcgc actatgaggg caccgggccc
      901 gagatctggc gtgacctgcc cgagatcacc cacttcgtcg ccggtctcgg taccagcggc
      961 accctgatgg gcaccgggcg ctacctcaaa gagcagaacc ccgacatcaa ggtgatcgcg
     1021 atcgagccgc cgttgggtga gcgggtcgag ggtttgcgca acctcgacga cggctacatc
     1081 ccgccgctgt tcgagacgtg gaacgggtac gagctgctcg accgcaagcg ggtcgtgcgc
     1141 ccgcgcgaga gccttgagtg ggctcgtcgt ctggtgtcgg agtgcggcct gttcgtcggt
     1201 ctctcggccg gcgcggcgat ggctggggcc gccaaggtgg ccgccgaggt cgacgccagc
     1261 gaggccgacg acaccgtgat cgcgttcatc gtcgccgacg gcggttggaa gtacctctcc
     1321 accggtgcct acaccgacga cctcgacgtg gccgaagccc gcgccgagga gatcatctac
     1381 ttctgatctg atgcctcgtc gggtgcggcg aacgaggcca ggatgttggc tccgccgatg
     1441 ccgacccgat gaccggtgcg ggagatgaat cccagctgga agccgatcgc cgcgtcgcgg
     1501 gtggtgaaga actcctcgtg gccgtcgtcg tcgacgacga tctcggtcaa cagcggtgca
     1561 tcggcggcgg tcagggcagc tcgcgcgtag tcggcgttga gcacgtcgac cgagacgtgt
     1621 tggatgccgc tgccgtgggc ggccaggtgc gcggccacgg catcgccagc ctctggcccg
     1681 accagcacga tcaccgcacc accggcctgc accgcctgga taccgggatg ctcgccgtcg
     1741 gccagcgcca ccgggtggaa gccgaaccga tcggtgaacg tggccgcagc cgcgccgagg
     1801 tcgtcgacga cgatgaccac gtgatcgagc cgggtgacga ccgtgtcgat cgcgctgcgg
     1861 gcggtgtccg gggcggccag cttgtcgggc ttgccgtcgg tgcgcgctcc ggcgacttcg
     1921 atgcgatggg tctcggccag cagcacccgc atcacctgtt cggcgaagtc cgggtcgacc
     1981 ccgctgtcga tcgcccactg gcggcgcgag gtgacgacgt cgcggacccg cgctggttgg
     2041 atgacgggcg tgtcggtgcc ttccttgacc cgggcgacct cttcacatac cgccaggcgt
     2101 tgggcgagca cgtcgatcag ttgccggtcg agttcgtcga tgcgggcgcg caggtcggcc
     2161 agggaggtca cacggtgcac ggtagcgaag agccacccgt accccctagc cttgccgggt
     2221 ccgttcgggg ctaccacgtt gctgccaggt catcctggcc tgccggttcg tgggtgcgct
     2281 ggcgtgatgc caccaggccg atcggcccgg ggcgacctcc tctggaaggc atataacacc
     2341 atggacaagc tcgacaccat cgctgagcac cgcctgcacg acaccgacac cggttctccc
     2401 gaggtgcaga tcgcgctgct gacccagcgc atcaaccacc tgaccgagca cctcaagttc
     2461 cacaagaagg accaccacag ccgtcgtggt ctgctgatgc tcgtcggcca gcgccgccgc
     2521 ctgctcgact acgtccgcgc ccgcgacatc gagcgctacc gcgcgatcat cgcccgcctg
     2581 ggattgcggc gctagtcgtc tccctcggtc gttcgctgcg ctcacgacct cggtcgagtt
     2641 gttcggagcg gctgcgccgc actcagttgc tgggcgggtc ggcggagccg acctgtccgc
     2701 ccggatcagg gggctaccgc cccccgaacc ccccgtcggg gtgtttccgc ggtgacgttg
     2761 ggcgaccgtc cacgtgtggg cgcgattgcc agcgcattgg acagtcccgt gcgcacggtt
     2821 tgactcgtgc gctagcgggt gcaactggca cgtggtgacc tcgcactggc ggcacggggt
     2881 ttgatccgtc tgtctgctgg cgcgacccgt gtgtgagggg cgggggtgtt tacacttcgg
     2941 cgccgggtga cttgtcggga ggggacacaa ctgatgagcg gggctgacgg gtctcgtcac
     3001 gaagggcgca acggcgggct ggcgtatctg ccggggctcg atgggttgcg cgcactcgct
     3061 gtggcggcgg tggtcgcctt tcactttggc gccggatggg caacgggggg cttcctcggt
     3121 gtttcgctgt tcttcacgct gtcggggttt ctgatcaccc gggtgctgct ggccgagcgc
     3181 agcgccgacg ggcacgtgcg gctgcgcggg ttctggggtc gacggatccg ccgcttgacc
     3241 ccggcggcga tggtgtgcct ggccggagta ttggtggtga tggccgtgcg cggcgacgcc
     3301 ggccatgccc gccaggtcgg ccacgacgtg ctggcgagtg cgttcaacgt cgccaactgg
     3361 cacttcgtgt cgagctcggt ctcatacggc gatctgttca gcgggtggcg gagcccagtg
     3421 ctgcacttct ggtcgctggc gatcgaagag cagttctacg tgctgttccc ggtgctgttc
     3481 gtggtggtcg cccgctggca cgaccggctc gatcgtcgtg ggctcgatcg tcgtgggctc
     3541 gatcgtcgcg ggcggcggtg gcggtcagcc ccggccgtgg tggctgtgac ggtggtcggg
     3601 gcgatcctcg cctcggtcgt cgcatcgtgg ctgaccgcgg acggcgacct ggtctacttc
     3661 ggcactcaca cccgcgccgg cgagttgttg atcggtgtgt tgctcgcctt ggcggtcgag
     3721 gc
//
LOCUS       DAORNF010000290         3711 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_18701_length_3711_cov_1.698578, whole genome shotgun sequence.
ACCESSION   DAORNF010000290 DAORNF010000000
VERSION     DAORNF010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3711
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18701_length_3711_cov_1.698578"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>257)
                     /locus_tag="PLV68_11880"
     CDS             complement(<1..>257)
                     /locus_tag="PLV68_11880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01991.1"
                     /translation="EDCGHALTATAVAAATTTLARAAGAPDATTLVPAPNGAPPPGAG
                     SGVEAGEPSRPAECAWCGSELIEDGFCTACGVKQRTWRDHW"
     assembly_gap    258..400
                     /estimated_length=143
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(623..2554)
                     /locus_tag="PLV68_11885"
     CDS             complement(623..2554)
                     /locus_tag="PLV68_11885"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRE01992.1"
                     /translation="MTQQRCLQPGCTGTILDGYCDVCGLAPSPMSRTAPTASGAAASA
                     MPGDADSYASARLLSTPIGSSRTAPGSRPTRRLGIGPATTRLGAGMTHVPPVPIVDPV
                     RSLMNPAEVPEEKRFCSVCGTEVGRSRPDRPGKAKGFCTKCRTPYDFEPKLKPGMLVG
                     GQYEVAGCLAHGGMGWIYLARDRNVNDRWVVLKGLLNAGDPDAYRAAVAEKQFLAEVE
                     HPLIVEIYNFVTAADGTSYIVMEYVGGKALNGILKERLAANGGVFDPLPPDQAIAFMI
                     EILPAFGYLHSVGLLYCDFKPANVIQSGDALKLIDLGGVRKVDDDVTPIYGTVGFQAP
                     EVAAEGCTVASDLYTVARTLAVLIFEFRGYQNTYADSMPGPDTVPLFAQHDSLFRWLL
                     KGTAIQPADRFQSADEMREQLLGVMREVTAALAVGRQGVVTRSTPSRLFSTPAIATDG
                     LNWNDLPTLLADPHDSMTDWLAGVTADDPLARLNALAAAPEQTAAVSIATAYAHLETG
                     DLAQADAVARRVLDADPWDWRGVWLMGLSALARRDWSGAVSAFNAVYGQVPGELAPKL
                     ALARACEETADAAVANRLYTTCAITDAAYVAPAQFGLARIAARSLDRVAALLALDSIP
                     ATSRAYGDAAAGHPARGRS"
     gene            complement(2551..>2918)
                     /locus_tag="PLV68_11890"
     CDS             complement(2551..>2918)
                     /locus_tag="PLV68_11890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01993.1"
                     /translation="VEDACRTFLHRLDRLDAALAEAERRFRAPLAERDDLRGLLRAYN
                     DKADESGLTEHPDLTVASRAAADLLWSAPCDLAEARRRVEHYIAAVNALTNGETPRVG
                     NRIGDDQADQAAPNQGERP"
     assembly_gap    2919..2928
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2929..>3315)
                     /locus_tag="PLV68_11895"
     CDS             complement(<2929..>3315)
                     /locus_tag="PLV68_11895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01994.1"
                     /translation="VAGHLAQLLGDQVRHIGALRRRADAAIAELGAAGGDTTAPEFTR
                     LTAIAAEIATARAGLENDDRERQAMIAAWSELPRRLAAANERETIVRDLRATCQAKVT
                     PVPNLAVPDVDALVPPLTIDEVERQPW"
     assembly_gap    3316..3325
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3326..>3711)
                     /locus_tag="PLV68_11900"
     CDS             complement(<3326..>3711)
                     /locus_tag="PLV68_11900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01995.1"
                     /translation="RLERTDATAFVAAVRGWLATMTDRLFAVDAQQQSLGPDAPDRIE
                     TDVAVAFVAHQAIGARLDVMGSPDGLGHVSAWCYQTVTASDGSVVTATLGDACVLLES
                     VIGATETTLARTATDVHETLRVRAAI"
BASE COUNT          504 a         1200 c         1321 g          523 t
ORIGIN      
        1 ccagtggtcg cgccaggtgc gctgcttgac gccgcaggcc gtacagaaac catcctcgat
       61 cagctccgat ccgcaccacg cgcactcggc tggtcgtgac ggctcaccag cctccacgcc
      121 ggaccccgca ccgggcggtg gcgcgccatt gggagccggg accaacgtgg tggcgtcggg
      181 tgctccggcg gcgcgggcca gggtggtggt cgccgcagcg acggctgtgg cggtgagggc
      241 gtgaccgcag tcctcgcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tggcgggcgg cgtcgcgata
      421 ggcgcgttcg agcgcgagcc gcagatcgac ctcggtcgcg ttgaccccgg ccacggtggt
      481 cccacccgtc ctggtgccag cgcgaccgtt gcgcacgagg tcgagtgcac tcgacaggat
      541 gtcgaggcat atcgacgtcc gttcggctgc ggtcaggttg ccgagctcga gctcggcagc
      601 ggcccgatcg aggtcgtcga gctcacgacc ggcctcgggc tgggtggcca gcagcagcgt
      661 cgccgtaggc ccggctggtg gcggggatcg agtcgagcgc gagcaaagcg gcgacccggt
      721 cgagcgaacg agcggcgatg cgcgccaggc cgaactgggc cggggcgacg taggccgcgt
      781 cggtgattgc gcacgtggtg tagaggcggt tggccacggc tgcatcggcc gtctcctcgc
      841 acgcccgggc caaggccaac ttgggtgcga gctcgccggg aacctggccg tagacggcat
      901 tgaaagcgct gaccgcgcca ctccagtcgc ggcgggccaa cgcagacaga cccatcaacc
      961 acacgccgcg ccagtcccac ggatcggcgt cgagcactcg ccgggcgacc gcgtcggcct
     1021 gggccagatc gccggtctcc aggtgggcgt aggcggtggc gatcgacacc gcagcggtct
     1081 gctctggcgc ggctgcgagc gcgttgagac gggcgagagg atcgtcggcg gtgaccccgg
     1141 ccagccagtc ggtcatcgaa tcgtgcgggt cggccagcaa cgtcggcagg tcgttccagt
     1201 tcaagccgtc ggtggcgatc gccggggtgc tgaacaagcg cgacggagtc gagcgggtga
     1261 cgactccttg gcgcccgacg gccagcgccg cggtgacctc gcgcatcacg cccagcagct
     1321 gctcgcgcat ctcgtcggcc gactggaacc ggtcggccgg ttggatcgcg gtgcccttca
     1381 gcaaccaccg gaacagcgag tcgtgctgag cgaacagcgg caccgtgtcg ggtccgggca
     1441 tcgagtcggc gtaggtgttc tggtagccgc ggaactcgaa gatcagcacc gccagcgtgc
     1501 gcgccaccgt gtacaggtcg ctggcgacgg tgcaaccctc ggccgcgacc tccggggcct
     1561 ggaacccgac cgtgccgtag atcggcgtca cgtcgtcgtc gaccttgcgc acaccgccca
     1621 ggtcgatcag cttcagcgcg tcgccactct ggatcacgtt ggcgggcttg aagtcgcagt
     1681 acagcagccc gacgctgtgc aggtagccga acgcaggcag gatctcgatc atgaacgcga
     1741 tcgcctggtc gggcggcagc ggatcgaaca cgccgccgtt ggccgcgagg cgctctttga
     1801 ggatgccgtt cagcgccttg ccgccgacgt actccatcac gatgtagctg gtgccgtcgg
     1861 cggcggtgac gaagttgtag atctcgacga tcagcgggtg ctcgacctcg gccaggaact
     1921 gcttctcggc caccgcggcg cggtaggcat cggggtcgcc cgcgttgagg aggcccttca
     1981 acaccaccca gcggtcgttg acgttacggt cgcgggcgag gtagatccag cccatgccgc
     2041 cgtgggccag gcatccggcg acctcgtact ggccgccgac gagcatgccc ggcttgagct
     2101 tcggctcgaa gtcgtacggg gtgcggcact tggtgcagaa gcccttggcc ttgccggggc
     2161 gatcgggtcg cgagcggccg acctcggtgc cgcacaccga gcagaaccgc ttctcctcgg
     2221 gcacctcggc cgggttcatc aggctgcgca ccgggtcgac gatgggcacc ggtgggacgt
     2281 gggtcatgcc cgcaccaagg cgggtggtgg ccgggccgat gccgagacga cgggtgggac
     2341 ggctgccggg cgcggtgcgc gacgaaccga tcggcgtcga gagcaggcgg gcgctggcgt
     2401 acgagtcggc atcgcccggc attgccgacg cggcagcacc tgatgccgtg ggtgccgtac
     2461 gggacatggg ggagggtgcc agcccgcaca cgtcgcagta cccgtcgaga atcgtgccgg
     2521 tgcagccggg ctggaggcag cgttgctggg tcatggacgt tctccctgat tcggagcggc
     2581 ctgatcggcc tgatcgtcgc cgatgcggtt gccaacccgt ggtgtctcgc cattggtcag
     2641 cgcgttgacc gcggcgatgt agtgctcgac ccgccgccgc gcctcggcca ggtcgcacgg
     2701 cgccgaccac agcaggtctg ccgcagcacg ggaggcaacg gtgaggtcgg ggtgctcggt
     2761 caggcctgac tcgtcggcct tgtcgttgta ggcccgcagc agaccgcgca ggtcgtcgcg
     2821 ctcggccagc ggtgcgcgga agcgccgctc cgcttcggcc agggccgcgt cgagccggtc
     2881 gagccggtgg aggaaggtgc ggcacgcatc ttcgaccgnn nnnnnnnncc acggttggcg
     2941 ctcgacctcg tcgatggtca gtggcggcac cagggcgtcg acgtcgggca cagccaggtt
     3001 cggcaccggg gtgaccttgg cctgacaggt ggcgcgcagg tcgcgcacga tggtctcccg
     3061 ttcgttcgcc gcggccaaac gccggggcaa ctccgaccag gctgcgatca tcgcctgccg
     3121 ctcgcggtcg tcgttctcca gcccggcgcg ggcggtcgcg atctcggccg cgattgcggt
     3181 gagccgggtg aactccggcg cggtcgtgtc gccaccggcc gcgcccagtt cggcgatggc
     3241 cgcgtcggcg cggcggcgca acgcaccgat gtggcgcacc tggtcgccca gcagttgggc
     3301 caggtggccg gcgacnnnnn nnnnntgatc gcggcccgta cccgcagggt ttcgtgaacg
     3361 tcggtcgcgg tgcgggccaa cgtcgtctcg gtggcgccga tcaccgattc cagcagcacg
     3421 cacgcgtcgc cgagggtggc ggtgacgacc gagccgtcgg atgcggtcac ggtctggtaa
     3481 caccacgcgc tcacgtggcc gagcccgtcg ggcgacccca tcacgtcgag ccgggcaccg
     3541 atcgcctggt gggcgacgaa cgcgaccgcg acgtcggtct cgatccgatc gggcgcatcg
     3601 ggaccgagcg actgctgctg cgcgtcgacc gcgaacagcc ggtcggtcat cgtcgccaac
     3661 cagccgcgca cggccgcgac gaacgcggtc gcgtcggtgc gctcgagccg t
//
LOCUS       DAORNF010000291         3704 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_18747_length_3704_cov_1.717731, whole genome shotgun sequence.
ACCESSION   DAORNF010000291 DAORNF010000000
VERSION     DAORNF010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3704
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18747_length_3704_cov_1.717731"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            179..1165
                     /locus_tag="PLV68_11905"
     CDS             179..1165
                     /locus_tag="PLV68_11905"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRE01996.1"
                     /translation="MRELYPGDFPPQRWAVANLGLVDRVKPKQQADGADHTVILFVGR
                     FEVRKGIDVVLDAISKIAPRYPDVRFVLAGEDRPLRPGEAMIGERWRAEHAGAPWLHQ
                     VHFAGVVEDEALHQLYAEADVALLPSRYESFGLVMAEAQMHGVPVVSSNVSGIPAVVR
                     DGVDGILVPPGDVDDVVNALMRLLSDRELRTRLGANGRERFVEQFDIERFADRFEQLF
                     ATVELQPISSKTVTGPHWWLRRDGTPPALRAHSSCAFTVATSSATEVRSLLMQAESDA
                     VVCIDGALHRLRPPDVHRHRLTDVHHGESIEVSVVEGAVVFGGVVTVRSEHR"
     gene            1165..2238
                     /locus_tag="PLV68_11910"
     CDS             1165..2238
                     /locus_tag="PLV68_11910"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRE01997.1"
                     /translation="MRVAIVSGVVVAHDAISAAVAKQASLLASMPGVERVTVFAQHID
                     RHLDVDGVVVGDPWQLVRLPSFAECDTVILHWGIFYTLFDVITLLSNRRVAVHFHNCT
                     PVELVPETERGGIVSSQTQIAHAISLGVRLWTFSDFNEQTLLSWGADRSRIDFVPFPI
                     DVPLTRPRRRSAGESVGLLSVGRFVPAKGQLTLLEAVAQLDVPLRQRLRVTLAGSRTF
                     SDVEYVRQIESAIAARDLADQVRIVEDADDVELWDLYRGHDVVISTSWHEGLCVPVIE
                     GYAAGCIAIGTTAGNVPFLVTADDEVTPPGDSSALVAAIERAVRRVDAGSTREQIERR
                     HSVARRFSSDSCRHAMSEALHVQ"
     gene            complement(2239..>2488)
                     /locus_tag="PLV68_11915"
     CDS             complement(2239..>2488)
                     /locus_tag="PLV68_11915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01998.1"
                     /translation="LRTVMSNFVAENRSTTAVIDMHSLLCRGANCEVSAGGHPVRPDG
                     LHLAGPGATTIAVNVLQHAIEPPTQGWPQVETISWASD"
     assembly_gap    2489..2524
                     /estimated_length=36
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2525..3608)
                     /locus_tag="PLV68_11920"
     CDS             complement(<2525..3608)
                     /locus_tag="PLV68_11920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE01999.1"
                     /translation="MAFHARLDRFYLGSDTRAFGILLGALLAVTATPRLRRIATWLSP
                     LAVGVLVVAVIWLDGSSLASFRGQFQVTSLAGAALICAVTGFGNSLLRTVLSNAILVS
                     VGKWSYGIYLFHWPLLLVLNERYELAGWGLATVVVFASTAFAFASFRWVESPLRNQGL
                     RAFRGPPLTLATAAVIVVVAVAATVVGARAPLTASAVVGQSVPTIPASPETPATVDTN
                     ATVGITPRPVDRPYRIMVVGDSVGASLVPQLDALSSQVGIEVFSRAAPSCSYDRVITV
                     GSAHFREDPLCVDIVEGWVADVASFRPDVVLFSYGSWSGWTYEGQFRTQCDPILAAYV
                     SHLYSLAVSDLGSFGAPVYMIQPAYWR"
BASE COUNT          703 a         1079 c         1207 g          679 t
ORIGIN      
        1 aaccgcgttg aaacgcttga tcaggtcctc cgtcacgccg accgcgatcg cggcggagat
       61 gtctggccag ttcgacggcg gagtcaatgc ggacgttgag cggctgatgg cgctcgaacg
      121 ctccgtgatg gatcaggccg atctgtttca ggccaacacc gaggctgttg tcgccacgat
      181 gcgggagctc tatcccggcg actttccgcc acagcgctgg gcggtggcaa acctcggtct
      241 ggttgaccgt gtcaagccca agcagcaggc agatggtgcc gaccacaccg tgatcctgtt
      301 cgtcggtcgg ttcgaggtac gcaagggcat tgacgtcgtg ctcgatgcaa tctccaagat
      361 cgctccccga tacccagatg ttcgctttgt gcttgcaggg gaagaccgtc cgctgcggcc
      421 cggcgaggcg atgatcggcg aaaggtggcg agcagagcac gctggggcac cctggctgca
      481 tcaggtgcac tttgctggcg ttgtcgaaga cgaggcgctg catcagctgt atgccgaagc
      541 cgacgttgct ttgttgccgt ctcggtacga gtcgttcggt ttggtgatgg ccgaagcgca
      601 gatgcacggt gtgcccgttg tcagcagcaa tgtgagtggt atcccggccg tcgtgcgcga
      661 tggcgtcgac ggcattctcg tgccgccggg cgatgtcgac gacgttgtca acgcgctgat
      721 gcgtctgctg agcgatcgag aattgcgtac gcgcctcggc gcgaacggtc gtgagcggtt
      781 tgtcgagcag ttcgacatcg agcgcttcgc cgaccgtttt gaacagctct ttgccaccgt
      841 ggagttgcag ccgatcagct cgaaaacggt gaccggtcct cactggtggt tgcgccgcga
      901 cggcacccca ccggcgctgc gcgcccactc atcatgtgcc ttcactgtgg cgacaagtag
      961 cgcgaccgag gtgaggtcac tgctgatgca ggcagaatcc gatgctgtcg tctgcatcga
     1021 cggtgcgttg catcgattgc gcccacccga cgtgcaccgt catcgactga ccgacgtcca
     1081 ccatggggaa agcatcgaag tatcggtcgt ggagggtgcg gtggtgttcg gcggagttgt
     1141 cacggtgcgc tcggagcacc ggtgatgcgc gttgcgatcg tgtccggtgt ggtggtcgct
     1201 cacgacgcaa tctcagctgc cgtcgcaaaa caagccagcc tcctcgcatc gatgcccgga
     1261 gtcgagcggg taacggtctt cgcgcagcac atcgaccgcc acctcgacgt agacggtgtc
     1321 gtcgtcggcg acccgtggca acttgtgcga ctcccgagct ttgctgaatg cgacaccgtc
     1381 attctgcact ggggcatctt ttacacgctg ttcgatgtga tcacgttgct gtccaatcgg
     1441 cgggttgcgg tgcactttca caactgcaca cccgttgagc tcgttccgga gacagagcgc
     1501 ggcggaatcg ttagctctca gactcagatc gcgcatgcca tttcgctcgg tgtgcggctg
     1561 tggacgttca gcgacttcaa cgagcagacg ttgttgtcat ggggagcgga tcggagccga
     1621 atcgactttg ttccgtttcc catcgatgtt ccgctcaccc ggccaagacg gcggtccgcc
     1681 ggcgagtcgg tcgggctgct gtcagtcgga cgtttcgtgc cagcgaaggg ccagctcacg
     1741 ttgctcgagg ccgttgccca gctcgatgtc ccgctgcgtc aacgactgcg agtgacgctg
     1801 gctgggagtc gtacgttctc cgatgtcgag tacgtgcgtc agatcgaatc tgcgatcgca
     1861 gccagggatc tcgcggacca agtccgcatt gtcgaggatg ccgacgacgt agaactctgg
     1921 gatctgtatc gcggccacga cgtggtcatc agcacctcat ggcacgaagg cctgtgtgta
     1981 ccagtgatcg agggatacgc agcgggctgc atcgcgatcg gcacaacggc agggaacgtt
     2041 ccctttctcg tcaccgcgga cgacgaggtg actccgccag gtgactcgtc cgcacttgtt
     2101 gcggcgatcg aacgagctgt acgtcgcgtt gacgccggtt caacacgaga gcagatagaa
     2161 cgacgccatt cggttgcgcg cagattttcg tccgactcat gtcggcatgc gatgagcgaa
     2221 gcacttcacg tgcagtagtc aatcgctggc ccaagagatc gtctcgactt gaggccagcc
     2281 ttgagtaggg ggttcgatgg cgtgttgtag cacgttgacg gcgatcgttg ttgccccggg
     2341 accggccaga tgcaggccgt ccgggcgaac ggggtgcccg ccagcggaaa cttcacagtt
     2401 ggcgccgcgg cacaggagcg agtgcatatc gatgacggcc gtcgtgctcc gattctctgc
     2461 aacgaagttg gacatcaccg tccgtaagnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnngtcgcc aatatgcagg ctgaatcatg tagaccggcg cgccgaagga tccgaggtca
     2581 ctcaccgcga gcgagtagag atgcgagacg taggcggcaa gtatcgggtc gcactgcgtc
     2641 cggaactggc cctcgtaggt ccagccggac cagctcccgt acgagaacaa cacgacgtcc
     2701 ggtcggaacg atgcgacatc ggcgacccag ccctcgacaa tgtcgacaca gagcggatcc
     2761 tcacgaaagt gcgcagatcc gacggtgatg acgcggtcat agctgcagga cggtgcggct
     2821 cgtgagaaca cttcgatgcc gacctggctt gaaagcgcgt caagctgcgg aacgaggctg
     2881 gctccgactg agtcgccgac caccatgatt cggtacggac ggtcaactgg gcgaggggtg
     2941 atgccaacgg tcgcgttggt gtcgactgtt gcgggtgtct cgggtgatgc tgggatcgtc
     3001 gggactgact gtcccaccac tgccgaagct gtgagtggcg cgcgtgcacc aaccacagtc
     3061 gccgcgacag cgacgacgac gatgaccgcc gctgttgcca gcgtgagagg tgggccgcga
     3121 aacgctcgga ggccttggtt gcgcagcggt gactcgaccc agcgaaagga ggcgaaggcg
     3181 aaggctgtcg acgcaaagac gacgactgtg gccaaccccc aaccggcgag ttcgtagcgc
     3241 tcgttcagaa cgagcaagag cggccagtgg aacaagtaga tgccatagct ccacttgccg
     3301 accgagacga gaatcgcatt ggacagcacg gtgcgtagaa gactgttgcc gaaaccagtg
     3361 actgcacaga tgagcgctgc tccggcgagt gatgtcacct gaaactgacc ccgaaatgac
     3421 gccagcgacg acccgtccaa ccagatgacg gcgaccacca gcacgccgac agcgagcggc
     3481 gacagccatg tcgctatgcg acgaagtcga ggtgtggcag ttacagcgag cagcgcgccg
     3541 aggagaatgc cgaacgcgcg tgtatcgctg ccgaggtaga agcggtcgag gcgggcgtgg
     3601 aacgccatcc acacttgcca cgacagcgct acagcggcga gtactaccag cgcgggcttc
     3661 cgacggctcc accgaagcac gacgactgcg agcaaggggt agag
//
LOCUS       DAORNF010000292         3696 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_18805_length_3696_cov_1.438066, whole genome shotgun sequence.
ACCESSION   DAORNF010000292 DAORNF010000000
VERSION     DAORNF010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3696
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18805_length_3696_cov_1.438066"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>265)
                     /locus_tag="PLV68_11925"
     CDS             complement(<1..>265)
                     /locus_tag="PLV68_11925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02000.1"
                     /translation="KEKTGGLDDTQLRTLAERTGGPDGSRRLAWTPEWIEARALFREA
                     LATLPVEIETDEAGNLWAYLRGASPDTVVVGSHLDSVPKGGWLD"
     assembly_gap    266..282
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            353..>789
                     /locus_tag="PLV68_11930"
     CDS             353..>789
                     /locus_tag="PLV68_11930"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRE02001.1"
                     /translation="MTIEIRVPTAADWPVICRVDARAFGNWYTDEDVELARPAHDISR
                     FRVAFDGEAMVGVAGSYALDVTLPGGTTVPFGGVTWVCVAATHRRQGIMRRVVEAVHA
                     DIDSRGEPVASLYASEGSIYRHLGYGPATARRVMTIDARHARLR"
     assembly_gap    790..942
                     /estimated_length=153
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <943..>1269
                     /locus_tag="PLV68_11935"
     CDS             <943..>1269
                     /locus_tag="PLV68_11935"
                     /inference="COORDINATES: protein motif:HMM:NF037610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02002.1"
                     /translation="RRDKPDGGASPAYYLGHADGFAVYRLTQNWNDGLPNHELVLEDF
                     AAITPAARLALWHTLLNMDLVGTITTRVLGVEDPLEQLLVNQRHARTVALNDGVWVKV
                     LDPAIA"
     assembly_gap    1270..1333
                     /estimated_length=64
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1334..1539
                     /locus_tag="PLV68_11940"
     CDS             <1334..1539
                     /locus_tag="PLV68_11940"
                     /inference="COORDINATES: protein motif:HMM:NF024920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sterol carrier protein domain-containing
                     protein"
                     /protein_id="HRE02003.1"
                     /translation="DGATCRKARTSPDLVTDGGSFSALLYGGVRPSALVAAQRMTARS
                     AAALARADLFFPSTPPPHCHTAY"
     assembly_gap    1625..1767
                     /estimated_length=143
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1768..>2020)
                     /locus_tag="PLV68_11945"
     CDS             complement(<1768..>2020)
                     /locus_tag="PLV68_11945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HRE02004.1"
                     /translation="GQDDYVLAVAAAGGNATRALIYVKEAFADLGAEPAIPVADIAAL
                     TTLETSGTVTATQAKTILAEIVANGGGDAAAIAKANGFET"
     assembly_gap    2021..2154
                     /estimated_length=134
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2155..3088)
                     /gene="gatB"
                     /locus_tag="PLV68_11950"
     CDS             complement(<2155..3088)
                     /gene="gatB"
                     /locus_tag="PLV68_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HRE02005.1"
                     /translation="MSTTNSVTNPIPGNLYTAPPATGDEILIDGYELVVGLEVHVELN
                     TATKLFSASANQFGDEPNTNIDPVTLGLPGALPVLNRHAVELAMRIGAALNCTIQPCV
                     FHRKNYFYPDMPKAYQISQYDQPLNVDGFLTLPGGKRIGIERAHLEEDTGKSTHFGGT
                     GGRIHGSTYSLIDFNRAGVPLVEIVSRPDIRTAEEAREYVSELRGILLAVDASDAKME
                     EGSMRCDANVSVHKPGEPYGTRCEIKNVNSIRSLGKAIDYEARRQIDMIESGETIRQE
                     TRHWDENDGRTHTLRTKEDADDYRYFLEPDLVPLA"
     gene            complement(3088..>3696)
                     /locus_tag="PLV68_11955"
     CDS             complement(3088..>3696)
                     /locus_tag="PLV68_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HRE02006.1"
                     /translation="DVAERLDAAFDALAAAGASIVDVEVPAFGYGLTAYYLIAPAEAS
                     SNLARYDGVRYGLRVNAPDTNSMYMATRAEGFGDEVQRRIMLGTYALSAGYYDAYYGK
                     ALKVRRLIANDFDRAYAKADVLLTPASPTVAFEFASKGDDPLAMYLCDVYTIPTNLAG
                     HPAMSVPFGTGANGLPVGVQVLAGTLGEPQMFRAAAALERSV"
BASE COUNT          448 a         1073 c         1114 g          550 t
ORIGIN      
        1 cgtcgagcca cccgcccttg ggcacactgt cgaggtgcga gccgaccacc accgtgtcgg
       61 ggctggcgcc gcgcaggtag gcccacaggt tgcccgcctc atcggtctcg atctcgaccg
      121 gcagcgtcgc cagcgcctcg cggaacaagg ctcgggcctc gatccactcc ggcgtccagg
      181 ccaggcggcg actgccatcg gggccaccgg tgcgctcggc cagcgtgcgc agttgcgtat
      241 cgtcgagccc gccggtcttc tccttnnnnn nnnnnnnnnn nncatggcgc gaacgctact
      301 gcggccgaaa ccagccgtgg cgcgggaacg ccgggctgcg agactcgccg ccatgacgat
      361 cgagatccgc gtccccaccg cagccgactg gccggtgatc tgtcgcgtgg acgcgcgagc
      421 gttcggcaat tggtacaccg acgaagacgt cgagctcgcc cgtccggccc acgacatctc
      481 ccggttccgg gtcgcgttcg acggtgaggc catggtcggc gtcgccggtt cgtacgcgct
      541 cgacgtgacg cttcccggcg gcaccaccgt tcccttcggc ggcgtgacgt gggtgtgtgt
      601 cgcggccacc catcggcggc agggcatcat gcgccgggtg gtcgaagcgg tgcacgccga
      661 catcgacagc cgaggcgaac cagtggcgtc gctgtacgcg tccgaggggt cgatctaccg
      721 tcatctcggc tacggcccgg ccaccgcgcg acgggtgatg accatcgacg cccgccatgc
      781 ccgactccgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncccgccgc gacaaacccg
      961 acggtggggc cagcccggcc tactacctcg gccacgccga cggctttgcc gtctaccggc
     1021 tcacccagaa ctggaacgac ggcctcccca accacgagct ggtcctcgag gacttcgccg
     1081 cgatcacccc tgcggcccgg ctcgcgttgt ggcacacgct gctcaacatg gacctcgtcg
     1141 gcacgatcac gacgcgggtg ctcggcgtcg aggacccgct ggagcagttg ctcgtcaacc
     1201 agcgccatgc gcggaccgtg gccctcaacg acggggtgtg ggtgaaggtg ctcgatccgg
     1261 caatcgcctn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnccgacgg agcgacgtgc cgcaaggcgc gcacctcgcc cgacctggtg
     1381 accgacggcg gctcgttcag cgcgctgctc tacggcggcg tgcgtccctc cgcgctggtc
     1441 gccgcacagc ggatgaccgc ccgctctgcc gctgcgctcg cccgcgccga cctgttcttc
     1501 cccagcaccc cgcccccgca ctgccacacc gcctactgac cggcgaaccg gcgcaccggc
     1561 aggcgctggt cagatcagga ggcgcgtttg gcttggagca gggcggtgac gagtttgccg
     1621 tcggnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnngtt tcgaagccat tggccttggc gatcgctgcc
     1801 gcatcgccgc cgccgttggc cacgatctcg gccaggatcg tcttggcctg cgtggccgtc
     1861 accgtgccgg aggtctccag tgtggtcaac gcggcgatgt cggccaccgg gatcgccggt
     1921 tcggcaccga ggtcggcaaa ggcctccttg acgtagatca gcgcccgtgt cgcattgccg
     1981 ccggcggcag cgacggcgag gacgtagtcg tcctgaccgc nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngggcga
     2161 gcggcaccag gtcgggttcg aggaagtagc ggtagtcgtc ggcgtcttcc ttggtgcgca
     2221 gggtgtgggt gcgcccgtcg ttctcgtccc agtggcgggt ctcttgacga atggtctcgc
     2281 cgctctcgat catgtcgatc tggcgccggg cttcgtaatc gatcgccttg ccgaggctac
     2341 ggatgctgtt gacgttcttg atctcacagc gagtgccgta cggctcgccg ggcttgtgca
     2401 cgctgacgtt ggcgtcgcag cgcatgctgc cctcttccat cttggcgtcg ctggcgtcga
     2461 ccgcgagcag gatgccgcgc agctccgaca cgtactcgcg tgcctcttca gcggtgcgga
     2521 tgtcgggtcg gctgacgatc tcgaccagcg gcacgccggc ccggttgaag tcgatcagcg
     2581 aataggtgct gccgtggatc cggcccccag tgccaccgaa gtgggtgctc ttgccggtgt
     2641 cctcttcgag gtgggctcgc tcgatgccga tccgcttgcc gccgggcagg gtcaggaacc
     2701 cgtcgacgtt gaggggctgg tcgtactggc tgatctgata cgccttgggc atgtccgggt
     2761 agaagtagtt cttgcggtgg aacacacagg gctggatcgt gcagttgagg gccgcgccga
     2821 ttcgcatcgc cagttcgacc gcgtggcggt tgaggaccgg cagtgcaccg ggcagaccga
     2881 gggtgaccgg atcgatgttg gtgttcggct cgtcaccgaa ctggttggca ctggcgctga
     2941 acaacttggt cgccgtgttc aactcgacgt gcacttcgag gcccacgacc agttcgtagc
     3001 cgtcgatgag gatctcgtcg ccggtcgcgg gtggtgccgt gtacaggttg cccgggatgg
     3061 ggttcgtcac cgagttggtg gtggacatca cacgctcctt tccagcgccg cggcggcacg
     3121 gaacatctgt ggttcgccga gagtgccggc cagcacttgt acgcccacgg ggaggccgtt
     3181 ggcgccggtg ccgaagggca cgctcatcgc ggggtggccg gccaggttgg tggggatcgt
     3241 gtagacgtcg cagaggtaca tcgccaacgg gtcgtcgccc ttgctggcga actcgaacgc
     3301 gaccgtgggc gacgcagggg tcaacaacac gtcggccttg gcgtaggcgc ggtcgaagtc
     3361 gttggcgatc aggcggcgca ccttgagcgc cttgccgtag taggcgtcgt agtagccagc
     3421 gctcagggcg taggtgccga gcatgatccg ccgctgcacc tcgtcgccga agccctcggc
     3481 ccgggtggcc atgtacatcg agttggtgtc cggggcgttg acgcgcaagc cgtagcggac
     3541 gccgtcgtag cgggccaggt tgctgctggc ctcggccgga gcaatgaggt agtaggcggt
     3601 gaggccgtag ccgaaggcag gaacctcgac gtcgacgatg ctcgcccccg ccgcggcgag
     3661 cgcgtcgaag gctgcgtcga gccgctcggc cacatc
//
LOCUS       DAORNF010000293         3692 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_18821_length_3692_cov_1.785812, whole genome shotgun sequence.
ACCESSION   DAORNF010000293 DAORNF010000000
VERSION     DAORNF010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3692
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18821_length_3692_cov_1.785812"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1027
                     /locus_tag="PLV68_11960"
     CDS             <1..1027
                     /locus_tag="PLV68_11960"
                     /inference="COORDINATES: protein motif:HMM:NF013087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HRE02007.1"
                     /translation="DRLDRAGDLVTPHVVRMAATLGLAEHVAAGADDVATLAAAAGVD
                     PARLARVLPLLVGADVLTMTGDRVGLGELGVVLAESEHATDELRHSSLEGRTALAAAG
                     LVDFVRSGVAPHDAVFGTDFWTGVRADAAGRDNFEHELAQWARHWVPLVADRLVALVV
                     AERSPEGSLDGAAAPCIADIGGGCGDLLVALGERIPGANGVLVELDEQLPAARRRVGE
                     SAAVDRIELVAGDFFGTWPEADVYVLAQVVHDWPDEAATAILRRAGEALRADPTRRVV
                     IVERDARHADASDVQMLLLFASAERSDTAMRTIAASAGIDLAPAVPIGAGFVAWVGRV
                     HSRAVGR"
     gene            1027..1608
                     /locus_tag="PLV68_11965"
     CDS             1027..1608
                     /locus_tag="PLV68_11965"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRE02008.1"
                     /translation="MGAANVAGSSASPGPKPRYSRAEIAAAAAEVPELTIAAVAKHLG
                     TAPSSLYRYFDSREQMVVAAVDSVIGALAPPRLGRSSQPWRTMLQREVSARWTMIAAH
                     PALIDAWWDLGWTAASSDARRSAIVARLVEAGFSAALADAVNDAVGGLIASAAETLLG
                     GADAVEVHRARRRLDLQVALLLDGVASRLAPHS"
     gene            complement(1634..3676)
                     /locus_tag="PLV68_11970"
     CDS             complement(1634..3676)
                     /locus_tag="PLV68_11970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02009.1"
                     /translation="MSLLLPAATTTTAAATTTTQAPPTTPDTALVAPDADTAAELASA
                     LAGIESGCDPLDTRLCLLPFPSNFNTVADATSDTGLRVNFLADTAAANSDGVAVDMTE
                     WNRNDGFSPNSQLLTYVPNLDAKQSALPSWVDLDQSLEDDASVVIIDSESGERVPLFA
                     ELDAKAESDTDRVLAIRPAVVLGEGRHYVVALRYLRDSTGELIDASAAFRVYRDRLDV
                     AELDDRRPIMEQNFSTLEAAGIERGDLYLMWDFTVASTRSLSERMLTIRDTALDGLGD
                     AAPAFTYTAAPGEDGSGIAVQLSGTYTVPNFLTGDGSPGNGFHYDDADDAGHPTDPNA
                     LPTQNGTVEVAFVCNVSDATIAATEPAHLVQYGHGLLGRATEINAGNVRAFANEHNIV
                     FCATKWAGMSEDDIGNAAATLGEISNFRTVADRLQQGVLNHIFLGRLMTRPGGLDSVA
                     ELKRADGSLMIDTAHLDYDGNSQGGIMGMMLTAVSPDIERAVLGVPGMNYSFLLPRSV
                     DFTDYEAIYKPAYPNDMDRMIGLNLLQMLWDRGEGAGYVQHITSDPYPGTQTKEVLLD
                     VAFGDWQVSELTAMIAARELGATIHRPITSSSLEVDPGWGLESTVYPSTGSGIVIWDS
                     GNEPIPLDGVPPTGVRDSHEDPRADPDVRTQKAAFLFEDTLIDVCGGQSCTADKRD"
BASE COUNT          483 a         1170 c         1411 g          628 t
ORIGIN      
        1 cgaccggttg gaccgggccg gcgacctggt cacgccgcac gtcgtccgca tggcggcgac
       61 gctcggattg gccgagcacg ttgccgctgg agcagacgac gtggcgacgc ttgccgctgc
      121 tgctggcgtg gacccggcgc gccttgcccg tgtgctgccg ctgctggtcg gggccgacgt
      181 gctcaccatg accggcgacc gggtcggtct gggcgagttg ggggtggtac tcgcggagag
      241 cgagcacgcg accgacgagt tgcgtcacag ctcgctcgaa ggacgcaccg cgctggccgc
      301 agcgggactg gtcgacttcg tccgctcggg agtcgcgccc cacgacgcgg tcttcggcac
      361 cgacttctgg acgggtgtac gcgccgatgc ggctgggcgc gacaacttcg agcatgagct
      421 tgcccaatgg gcgcggcatt gggtgccgct cgtggcggac cgcctcgtcg ccctggttgt
      481 cgctgagcgc agtcccgagg ggtcgctcga cggtgccgct gcgccgtgta tcgccgacat
      541 cggcggggga tgcggtgacc tgctcgtcgc gttgggcgaa cgcattcccg gcgcgaacgg
      601 tgtgttggtc gagctcgacg aacaactgcc tgcggcgcgc cggcgtgtgg gcgagtctgc
      661 tgccgtcgat cggatcgaac tggtcgccgg cgacttcttc ggtacgtggc cagaggcaga
      721 tgtctacgtg ctggcccagg tcgtgcacga ttggccggac gaggccgcga ccgcgatctt
      781 gcgccgggcg ggcgaggcgt tacgtgccga tcccaccagg cgggtggtga tcgtcgagcg
      841 cgacgcgcgt cacgccgacg cgagcgacgt gcagatgctg ttgctgttcg cgagcgccga
      901 acgatccgat acggcgatgc ggacgatcgc cgcatcggcc gggatcgatc tggccccagc
      961 tgtgcccatc ggcgccgggt tcgtcgcctg ggtcggccgg gtgcactccc gcgcggttgg
     1021 ccgctgatgg gggcagcgaa cgtggccggg tcgtccgcgt cgcccgggcc gaagccgagg
     1081 tacagccggg ctgagatcgc ggccgccgcg gccgaggtac ccgagttgac catcgctgcg
     1141 gtggccaagc atctcggcac ggcaccttcg tcgctgtatc ggtacttcga cagccgcgag
     1201 cagatggtgg tcgcggcggt cgacagcgtg atcggcgcac tggcgccgcc tcgcctcggc
     1261 cgatcgagcc agccctggcg gacgatgttg cagcgcgaag tcagcgcgcg ctggacgatg
     1321 atcgcagcgc atcccgcgtt gatcgacgcc tggtgggacc tgggctggac ggcggcttcg
     1381 tccgacgctc gccgcagcgc gatcgttgcc cgcttggtcg aggcagggtt ctccgctgca
     1441 ctcgccgacg cggtcaacga cgcggtcggt ggactgatcg cgtcagcggc ggaaacgctg
     1501 ctcggcggcg ctgatgcagt tgaggttcac cgggcgcgcc gtcgcctcga cctgcaggtc
     1561 gcgctgttgc tcgacggtgt cgccagccgc ctcgcccctc actcctgacc gcgaggggga
     1621 ggggccgagc ggatcagtcg cgcttgtcgg cggtgcagga ctggccgccg cagacgtcga
     1681 tcagggtgtc ttcgaacagg aaggcagcct tctgggtgcg cacgtccggg tcggcgcgcg
     1741 ggtcctcgtg cgaatcgcgc acgccggtcg ggggcacgcc gtcgagcggg atcggctcgt
     1801 tgccgctgtc ccagatgacg atgcccgagc ccgtgctggg gtagaccgtg gactccagac
     1861 cccacccggg atcgacctcg agactgctcg acgtgatcgg gcggtggatc gtggcgccca
     1921 gttcgcgggc ggcgatcatc gccgtcaact cgctgacctg ccagtcgccg aacgcgacgt
     1981 cgagcagtac ttccttggtc tgcgtgccgg ggtacgggtc ggacgtgatg tgctgcacgt
     2041 acccggcacc ctcgccacgg tcccacagca tctgcagcag gttgaggccg atcatgcggt
     2101 ccatgtcgtt ggggtaggcc ggcttgtaga tcgcctcgta gtcggtgaag tcgacgctgc
     2161 gcggcaacag gaagctgtag ttcatgccgg gtacgccgag cacggcgcgc tcgatgtcgg
     2221 gtgacaccgc ggtcagcatc atgcccatga tgccgccctg gctgttgccg tcgtagtcga
     2281 ggtgggcggt gtcgatcatc agcgacccgt cggcccgctt cagctcggcc accgagtcga
     2341 gcccgccggg acgggtcatc agacggccga ggaagatgtg gttgagcacg ccctgttgca
     2401 ggcggtcggc aacggtgcgg aagttggaga tttcaccgag cgtcgcggcc gcgttgccga
     2461 tgtcgtcctc gctcatgccc gcccacttgg tggcgcagaa cacgatgttg tgttcgttgg
     2521 cgaaggcccg gacgttgccc gcgttgatct cggtggcccg gccgagtagg ccgtggccgt
     2581 actgcaccag gtgggcaggc tcggtggccg cgatcgttgc atccgaaacg ttgcagacga
     2641 acgcgacctc gaccgtgccg ttctgggtgg gtagcgcgtt ggggtcggtg gggtggccgg
     2701 cgtcgtccgc atcgtcgtag tggaagccgt tgccggggct gccgtcgccg gtgaggaagt
     2761 tgggcacggt gtaggtgccg ctgagttgca ccgcgatgcc cgaaccgtcc tcgcccggtg
     2821 ccgccgtgta ggtgaacgcg ggagcggcgt cgcccaggcc gtcgagcgcg gtgtcgcgga
     2881 tggtgagcat ccgctcgctg aggctgcggg tgctggcgac ggtgaagtcc cacatcaggt
     2941 acaggtcgcc acgctcgatg ccggcggcct ccagcgtgga gaagttctgc tccatgatcg
     3001 ggcggcggtc gtcgagctcg gcaacgtcga ggcggtcgcg gtagacgcgg aacgcggcgc
     3061 tggcgtcgat cagctcgccg gtggagtcgc gtaggtagcg cagggcgacg acgtagtgac
     3121 ggccctcgcc cagcacgacg gcggggcgga tcgccagcac gcggtcggtg tcggactcgg
     3181 ccttggcgtc gagttcggcg aacaacggca cgcgctcgcc gctctcgctg tcgatgatga
     3241 cgacgctggc gtcgtcctcc agcgactggt cgagatcgac ccagctgggc agcgcgctct
     3301 gcttggcgtc gaggttgggc acgtaggtga gcagttggct gttggggctg aacccgtcgt
     3361 tgcggttcca ctcggtcatg tcgacggcca caccgtcgga gttggcggcc gcggtgtcgg
     3421 ccaggaagtt gacccgcagg ccggtgtcgc tggtggcgtc ggcgacggtg ttgaagttgc
     3481 tggggaacgg cagcaggcac aggcgggtgt cgagcgggtc gcagcccgac tcgatgcccg
     3541 cgagcgcgct cgccaactcc gccgcggtgt cggcgtcggg ggcgacgagg gcggtgtcgg
     3601 gcgtggtcgg cggggcctgc gtggtggtcg ttgccgcggc ggtggtggtc gtggccgccg
     3661 gcaacagcag cgacatcgcc gacttctata cc
//
LOCUS       DAORNF010000294         3677 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_18932_length_3677_cov_1.969630, whole genome shotgun sequence.
ACCESSION   DAORNF010000294 DAORNF010000000
VERSION     DAORNF010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3677
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18932_length_3677_cov_1.969630"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="PLV68_11975"
     CDS             <1..212
                     /locus_tag="PLV68_11975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02010.1"
                     /translation="ALIRPSRGQWFLWQGEPAVAPARQAVLLEMNAMNPREVPTAAER
                     PGPHTVAVVRRQAQAVVAALHTHVP"
     gene            complement(256..819)
                     /gene="dcd"
                     /locus_tag="PLV68_11980"
     CDS             complement(256..819)
                     /gene="dcd"
                     /locus_tag="PLV68_11980"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HRE02011.1"
                     /translation="MILSDRSLRAEIAAGRIVIEPLDDACIQPSSIDVKVDNLFRVFR
                     NHTTGIIDVKQNLEDLTELVSIPDGGVFMLHPGEFVLGSTLERVVVPDNLVGRIEGKS
                     SLGRLGLLIHSTAGFIDAGFDGHITLELSNVASLPITLYPGMKIGQISFVQMTTAADN
                     PYGQGARGSKYQGQRGPTPSRYHENFG"
     gene            complement(853..1002)
                     /locus_tag="PLV68_11985"
     CDS             complement(853..1002)
                     /locus_tag="PLV68_11985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02012.1"
                     /translation="MLAAYLAARYPAADDPVWDDPGYPIRPARAAATVKRTQQLTYPV
                     IIESA"
     gene            complement(1031..2506)
                     /locus_tag="PLV68_11990"
     CDS             complement(1031..2506)
                     /locus_tag="PLV68_11990"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HRE02013.1"
                     /translation="MKERRKTATSNFGVGARENHDSTAFYDRFRPPVLSKDETVLAPV
                     ELDDPFFLGDARQMDGIEDGSVALVVTSPPYFAGKQYEEELEREGIPSTYLEYLDLLR
                     DVFAECVRKLEPGGRLAVNVANLGRKPYRSLSADVIQILERDLGLLLRGELIWQKGEG
                     ASGSCAWGSFRSASNPVLRDLTERIIVASKGRFDRAQTVKQRAAAGLPHESTLMTEDF
                     MALTLDVWSIPPESARRVGHPAPFPVELPEQLIRLYTFKGDLVLDPFMGSGSSLVAAA
                     NLERRYAGYDLDPAYVEIARQRVAEVHVPPPAMPVATHRRQKLDPPDQSVDDDFQSRA
                     TREGKAAQKLAREVLEHAGFRITETNQRIPKTGVTVHFVAIDDNDCRWFFDVPGAFTS
                     YRGGLLRTDAVWRSLGRANALKGRVGDVPIVFLTTDLPKRPSEGDTALRAAGPGAFFD
                     AIDMLSDVGRERLERYAKGGFVDNPQPGFWTPEDLARRARP"
     gene            complement(2548..2621)
                     /locus_tag="PLV68_11995"
     tRNA            complement(2548..2621)
                     /locus_tag="PLV68_11995"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2587..2589),aa:Gly,seq:ccc)
     gene            complement(2672..>3677)
                     /locus_tag="PLV68_12000"
     CDS             complement(2672..>3677)
                     /locus_tag="PLV68_12000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02014.1"
                     /translation="AISLNVTATRSTQAGFITVYACGTREFVSSVNFEAGQTVPNAVI
                     TPISPDGEVCFYSSVPVDVVVDVNGWFTANRAFTAVGPVRVFDTRPGQSPDAVRSAEV
                     PKQRIKGGELLEVKLTDLAGYVPAAGVGAVSINVTAANPSAAGFITVYPCGTRDFVSS
                     VNYLAAQNAPNAVITPVSATGTVCFFSTADTDLVVDINGWLKSPSGFTSVSPKRVFDT
                     RPGQTAPVLRAVETRQVGGTYVLEVKVTDLAGVTPATGVSAVSLNVTATNPGGPGFIT
                     VDACGVRREVSSLNYATGETIANAVLAPLSPTGTICFYAQTPVDIVVDINGWFTDQPS
                     "
BASE COUNT          620 a         1195 c         1239 g          623 t
ORIGIN      
        1 ccgcgctgat ccgcccgtca cgcgggcagt ggttcttgtg gcagggcgaa cccgccgttg
       61 ctccggcacg tcaggccgtg ctgttggaga tgaacgcgat gaacccgcgc gaggtgccga
      121 ccgcggctga acgtcccggc ccacacaccg tcgccgtggt gcgccgccaa gcccaagccg
      181 tcgtcgccgc cctccacacc cacgtcccct agcgcgatcg accggacgag cgagcgtgcg
      241 cgtgtgcggg ctggctcagc cgaagttctc gtggtagcgg ctgggggtgg gcccgcgttg
      301 gccctgatat ttgctgcccc gcgcgccctg cccgtagggg ttgtcggccg cggtggtcat
      361 ctgcacgaag ctgatctggc cgatcttcat gccgggatac agggtgatcg gcaagcttgc
      421 gacgttgctc agttccaacg tgatgtggcc gtcgaagccg gcgtcgatga accctgccgt
      481 cgagtggatc agcagaccga ggcgcccgag cgacgacttg ccctcgatgc gcccgaccag
      541 gttgtcgggc acgaccacac gctcgagcgt cgaccccaac acgaactcgc ccgggtgcag
      601 catgaacacg ccgccgtcgg ggatcgagac gagctcggtg agatcctcca ggttctgctt
      661 gacgtcgatg atgcccgtgg tgtggttgcg gaagacccgg aacaggttgt ccaccttgac
      721 gtcgatcgac gagggctgga tgcatgcgtc gtcgagtggc tcgatgacga tgcggccggc
      781 cgcgatctcg gcgcgcaacg agcggtcgga gaggatcacc cgcccagcct actgatgcgc
      841 ctcaacgcga cgtcatgccg actcgatgat caccggatag gtcagctgtt gcgtgcgctt
      901 caccgtcgcc gcggcgcgag cggggcgaat gggatagccc ggatcgtccc acaccggatc
      961 gtcggccgcg ggatatcgag cggccagata ggcggccaac acatgcgggg ccgcgccgcg
     1021 cttgccgcca tcagggccgt gcgcgtcggg cgagatcctc gggggtccag aagccgggtt
     1081 gcggattgtc gacgaatcca ccctttgcgt agcgctccag ccgctcgcga ccgacgtctg
     1141 agagcatgtc aatggcgtcg aagaacgcgc caggtccggc ggcccgcaat gccgtgtcgc
     1201 cctcactggg gcgcttgggg agatccgtcg tgaggaagac gatcggcacg tctcccaccc
     1261 gacccttcag agcgttcgcg cggccgagtg acctccagac cgcgtcggtg cgcagcaagc
     1321 caccgcggta gctggtgaat gctcccggaa cgtcgaagaa ccagcggcaa tcgttgtcgt
     1381 cgatggcgac gaagtgaacc gtgacaccgg ttttcgggat gcgctggttc gtctccgtga
     1441 tgcgaaagcc agcgtgctcg agaacctctc gggcgagctt ctgggcggcc ttgccttcgc
     1501 gggtggcacg gctctggaag tcgtcatcca ccgattgatc gggtgggtcg agcttctggc
     1561 gccggtgtgt ggccaccggc atcgccggcg gcggcacatg tacctcggca acccgttggc
     1621 gggcaatctc gacgtaggcg gggtcgaggt cgtagccggc ataacgccgc tccaggttcg
     1681 cagccgcaac cagcgacgag ccgcttccca tgaacggatc aaggacgaga tctcccttga
     1741 acgtgtagag gcggatcagc tgttcgggca gctcgacagg aaagggcgcc gggtgtccca
     1801 ccctgcgagc gctctcgggc gggatggacc agacatcgag tgtcagcgcc atgaagtcct
     1861 ctgtcatcag cgtgctctcg tgcggaagtc ccgccgcggc tctctgcttg accgtctggg
     1921 cgcgatcgaa tctgcctttg ctggccacga tgattcgctc ggtgaggtcc cgcagcaccg
     1981 gattggacgc acttctgaac gacccccacg cgcatgaacc gctcgcaccc tcgccctttt
     2041 gccagatgag ttcaccgcgc aacagcaagc cgagatcgcg ttcgaggatc tggatcacgt
     2101 ccgccgacag gcttcggtac ggtttgcggc cgagattggc cacgttgacc gcaagacgcc
     2161 cgcccggctc gagttttcga acgcattcgg cgaacacatc tcggaggagg tcgaggtact
     2221 cgaggtaggt cgatggaatg ccttcgcgct ccaactcctc ttcatactgc ttgccggcaa
     2281 agtacggagg tgaggtgacg accagggcga ccgacccgtc ctcgatgccg tccatctgtc
     2341 gcgcgtcacc caagaagaac gggtcgtcga gttcgacggg cgcgaggacc gtctcgtcct
     2401 tggacaggac ggggggccgg aagcgatcgt agaacgccgt cgagtcgtgg ttctcccgcg
     2461 cgcccacgcc gaagttcgac gtcgcggtct tccgtcgctc tttcatcgga ccactcctcc
     2521 tggctcctca gatgcggcgg agacgactgg agcgggtagc gggaatcgga cccgcgtatc
     2581 ttgcttggga agcaagcgct ctaccactga gctatacccg caaaggtgtg cgcagcctag
     2641 cgagtccagg ctgcgcaccc acagcgatgg gtcagctcgg ctggtcggtg aaccagccgt
     2701 tgatgtcgac cacgatgtcg acgggcgtct gcgcgtagaa gcagatcgtg cccgtcggcg
     2761 acaacggcgc cagcaccgcg ttggcgatcg tctcacccgt ggcgtagttc aggctggaca
     2821 cctcacgccg cacaccacag gcatcgacgg tgatgaagcc gggcccgcct gggttggtgg
     2881 cggtgacgtt cagcgacaca gcgctcacac cggtggccgg agtgaccccg gccaggtcgg
     2941 tgaccttgac ctccagcacg tacgtgccgc cgacctggcg ggtctcgacg gcgcgcaaga
     3001 ccggtgcggt ctgacccggt cgggtgtcga acacacgctt ggggctgacc gaggtgaagc
     3061 cgctcgggct cttcaaccac ccgttgatat cgaccaccaa gtcggtgtca gcggtgctga
     3121 agaagcacac cgtaccggtc gccgacaccg gcgtgatcac cgcgttcgga gcgttctgcg
     3181 ccgcgaggta gttcacgctg gacacgaagt cacgagtgcc gcacgggtac accgtgatga
     3241 agccggccgc ggacgggttg gccgccgtga cgttgatcga cacggctccg actccggcgg
     3301 ccgggacgta cccggccaga tcggtcagct tcacctcgag caactcgccg cccttgatcc
     3361 gctgcttcgg cacctcggcg ctgcgcacgg cgtcggggct ctggccaggc cgggtgtcga
     3421 acacacggac cggaccgacc gcggtgaacg cacggttggc ggtgaaccaa ccgttgacgt
     3481 cgaccaccac gtcgactggc acgctggagt agaagcacac ctcgccgtcg ggcgagatcg
     3541 gtgtgatcac ggcgttcggc acggtctggc cggcttcgaa gttgacgctg gacacgaact
     3601 cacgggtacc gcaggcgtac accgtgatga acccggcttg ggtggagcgg gtcgcggtca
     3661 cgttcaacga gatcgcg
//
LOCUS       DAORNF010000295         3670 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_18982_length_3670_cov_2.025450, whole genome shotgun sequence.
ACCESSION   DAORNF010000295 DAORNF010000000
VERSION     DAORNF010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3670
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18982_length_3670_cov_2.025450"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>267)
                     /locus_tag="PLV68_12005"
     CDS             complement(<1..>267)
                     /locus_tag="PLV68_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HRE02015.1"
                     /translation="SFWHNDEANASAGKKAFVTNRVGDLGMLIAMFFAFQYVGSLSYE
                     AINHAAEEGSFARSAATAMVAALFVGACGKSAQLPLYIWLPDAM"
     assembly_gap    268..310
                     /estimated_length=43
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<311..733)
                     /locus_tag="PLV68_12010"
     CDS             complement(<311..733)
                     /locus_tag="PLV68_12010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HRE02016.1"
                     /translation="MIELAWLIPALPLAGFLTILVLGRKLGDPAAGYLATTAIGGAFV
                     VAAAVFFDLLGQPVEARHHVIDLFQWIPVGDLQVDLAFQVDQLSITMLLFITGIGALI
                     HLYAVGYMHGDPKFSKFFLYLNLFVLSMTLLVMGENLLV"
     gene            complement(741..>1537)
                     /gene="nuoK"
                     /locus_tag="PLV68_12015"
     CDS             complement(741..>1537)
                     /gene="nuoK"
                     /locus_tag="PLV68_12015"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HRE02017.1"
                     /translation="HPVHCALSLIATLFGIAVLFVAMEAHFLAAAQIIVYAGAIVVLF
                     LFVIMLLGVDRVESLRIEPISAQRPLAGIVGVGLIAVIGAAVIESRDSIKARGTGLDV
                     AGADNPPDRPRPVQQPRVRLRAHVGFVGDRRRRHRVAGPAPEPAWPQRHRRTRQGGRQ
                     VIAVAPTTTWYLLLAAVLFGIGVVGLLVRRNPLVMFMCVELMLNAVNLSFVALARALG
                     DIGGQTVVFFVMVVAAAEVVVGLGIIVSILRKRPTATADDLAELKG"
     assembly_gap    1538..1561
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1581..2231)
                     /locus_tag="PLV68_12020"
     CDS             complement(1581..2231)
                     /locus_tag="PLV68_12020"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HRE02018.1"
                     /translation="MGYFEGFGVVIRQHRLFGGKRITKAYSGGRVFKRKPAKADVDDV
                     KVPKPERLHGRHVLNRYEDGMEKCIGCELCAGVCPAKCIYVRGADNDPLQPTSPGERF
                     GFVYEINYLRCIHCDMCVEACPTEAITETKLFEFSFTSRQDAVYTKDELLVGDDGKPR
                     AQPWEDWRAGEDLHTSGWMRATAPGGAAEFEGIPGWSSELGYGTRAPEPDQATRDA"
     gene            complement(2231..3454)
                     /locus_tag="PLV68_12025"
     CDS             complement(2231..3454)
                     /locus_tag="PLV68_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HRE02019.1"
                     /translation="MLALDPVLFDIDFGKSIVIVAIKVLIIFVIGLVGTMLMVWFERK
                     IIAGMQNRIGPNKAGPYGLLQTLADGMKLIFKEDLLPNRADRIVFRVAPFLAFVPAFL
                     VWSVLPLGGDFRDGKSGVIRIFGKDTLAQLADPPMGILLLLALSGIAVYGIVLAGWAS
                     GSKYPLLGSVRATAQMVSYEASLGLSIAAVILVAGTLSTHAIVAAQSGLSNWMVVSTG
                     VVPFVLFLISATAELNRPPFDLVEAEQELVGGFNTEYSAFMFALFYLAEFMNSVTMSG
                     VIVTLFFGGPQPLSFKIGALEYGGWLPFGPLSGTVWFLLKLLVFLYVYVWLRGTLPRL
                     RYDQLMNLGWKVLIPSSLGWFIVLAAQRVGDDNDWNPVVVTVITLAVLAACAGLLLTA
                     VRTSSKNREREGAMF"
     gene            complement(3454..>3670)
                     /locus_tag="PLV68_12030"
     CDS             complement(3454..>3670)
                     /locus_tag="PLV68_12030"
                     /inference="COORDINATES: protein motif:HMM:NF013717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HRE02020.1"
                     /translation="VNPLDVARIGAPVGSEVKVTSPKAMVVLALHTDPGVQRGTAWVP
                     FNQPGPKRVGDLIDLSAAVTDVRIETL"
BASE COUNT          686 a         1173 c         1218 g          526 t
ORIGIN      
        1 tccatcgcgt cgggcagcca gatgtacagc ggcaactggg cgctcttgcc acaggcgccg
       61 acgaacagag ccgcgaccat ggcggtcgcc gcgctgcggg cgaaggaacc ttcttcggcg
      121 gcatggttga tcgcctcgta gctcaacgac cccacgtact ggaaggcgaa gaacatcgcg
      181 atcaacatgc ccaggtcgcc gacgcggttg gtgacgaacg ccttcttgcc cgccgatgcg
      241 ttggcctcgt cgttgtgcca gaagctgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn gaccagcagg ttctcgccca tcaccaacaa cgtcatgctc agcacgaaca
      361 ggttgaggta gaggaagaac ttgctgaact tgggatcgcc gtgcatgtag ccgacggcgt
      421 acaggtggat cagcgcgccg atgccggtga tgaacagcag catcgtgatc gacagctggt
      481 cgacctggaa cgcgaggtcg acctgcaggt caccgaccgg gatccactgg aacagatcga
      541 tgacgtgatg gcgggcttcg accggctggc cgagcaggtc gaagaacacc gcggcggcga
      601 ccacgaacgc cccgccgatc gcggtggtgg cgaggtagcc cgccgccggg tcgccgagtt
      661 tgcggccgag caccaggatg gtcaggaacc cggccagcgg cagggcaggg atgagccagg
      721 caagttcgat catgagaggc ctagcccttc aactcggcga ggtcgtcagc cgtggcggtc
      781 ggccgcttgc gcaggatgga cacgatgatg ccgaggccga cgacgacttc ggccgcggcg
      841 acgaccatca cgaagaacac gaccgtctgg ccaccgatgt cacccagggc ccgcgccaag
      901 gcgacgaagc tgaggttgac cgcgttgagc atcagctcga cgcacatgaa catcaccagc
      961 gggttgcggc gcaccagcag cccgacgacg ccgatgccga acagcaccgc ggccagcagc
     1021 agataccagg tggtggtggg ggcgacggcg atcacttggc gcccgccttg gcgggtacga
     1081 cggtgtcgtt ggggccacgc cggctcgggc gccgggccag caacacggtg ccgacgacgg
     1141 cgatcaccaa caaaaccgac gtgagctcga aggcgaacac gtggttgctg aacaggtcgc
     1201 gggcgatctg gcgggttgtc ggccccggcg acgtcgagcc cagtgccgcg ggccttgatc
     1261 gaatcgcggc tctcgatcac ggccgcgccg atcacggcga tgagtccgac gccgacgatg
     1321 cccgccagcg gtcgctgggc gctgatcggc tcgatgcgca acgactcgac gcgatcgacg
     1381 ccgagcaaca tgatgacgaa caggaacagc accacgatcg cgccggcgta gacgatgatc
     1441 tgcgcggcag cgaggaagtg ggcctccatc gccacgaaca gcacggcgat gccgaacagc
     1501 gtggcgatca ggctgagggc gcagtgcacc gggtgcgnnn nnnnnnnnnn nnnnnnnnnn
     1561 ngaagacgaa cagttccatg tcaggcgtcc ctcgtcgctt ggtccggctc gggggcgcgg
     1621 gtgccgtaac ccagctcgct gctccacccg gggatgcctt cgaactcggc cgcaccgccg
     1681 ggagcggtgg cccgcatcca accgctggtg tgcaggtctt caccggcgcg ccagtcctcc
     1741 cacggctggg cgcggggctt gccgtcgtcg ccgaccagca gctcgtcctt ggtgtagacc
     1801 gcgtcctgac ggctggtgaa gctgaactcg aacagcttcg tctcggtgat ggcctcggtc
     1861 ggacaggctt cgacgcacat gtcgcagtgg atgcagcgga gatagttgat ctcgtacacg
     1921 aacccgaatc gctcgcccgg cgaggtcggc tgcagcgggt cgttgtcggc accgcgcacg
     1981 tagatgcact tggccgggca gacgccagcg cacagctcgc agccgatgca cttctccatg
     2041 ccgtcctcgt accggttgag cacgtggcgg ccgtggaggc gctcgggctt gggtaccttg
     2101 acgtcgtcga cgtcggcttt ggccggcttg cgcttgaaca cccgcccacc ggagtaggcc
     2161 ttggtgatcc gcttaccgcc gaacagccgg tgctggcgga tgacgacgcc gaacccttcg
     2221 aagtagccca tcagaacatc gccccctctc gctcgcggtt cttggaactg gtgcgcaccg
     2281 cagtgagcaa caagcccgcg caggccgcca acacggccag cgtgatgacg gtgacgacga
     2341 ccgggttcca gtcgttgtcg tcaccgacgc gctgcgcggc cagcacgatg aaccatccga
     2401 gcgacgacgg gatcaacacc ttccagccga gattcatcag ctggtcgtag cgcagccggg
     2461 gcagcgtgcc gcgcaaccac acgtagacgt acaggaacac cagcagcttg agcaggaacc
     2521 agacggtgcc gctgagcggg ccgaacggca accagccccc gtactcgagc gcgccgatct
     2581 tgaacgacag cggctgcgga ccgccgaaga acagggtgac gatgacaccg ctcatcgtga
     2641 cgctgttcat gaactcggcc aggtagaaca gggcgaacat gaacgccgag tactcggtgt
     2701 tgaaaccgcc gaccagctct tgttcggctt cgaccaggtc gaacggcggg cggttgagct
     2761 cggcggtggc cgagatgagg aagagcacga acggcacgac gccggtcgac acgaccatcc
     2821 agttgctcaa cccgctctgc gcggcgacga tcgcgtgggt gctgagcgtg cctgccacca
     2881 ggatgacggc agcgatgctg agcccgagcg acgcctcgta cgacaccatc tgggccgtgg
     2941 cccgcaccga cccgagcaac gggtacttcg accccgacgc ccatccggcg agcacgatgc
     3001 cgtacaccgc gatgccgctg agggccagca gcagcaagat gcccatcggc gggtcggcca
     3061 actgggccaa cgtgtccttg ccgaagatgc ggatgacacc gctcttgccg tcgcggaagt
     3121 cgccgcccaa cggcagcacc gaccacacca ggaacgccgg cacgaacgcc aggaacggag
     3181 cgacccggaa gacgatgcgg tcggcccggt tgggcagcag gtcttccttg aagatcagct
     3241 tcatgccgtc ggccagggtc tgcagcaggc cgtacgggcc ggccttgttg ggaccgatgc
     3301 ggttctgcat gccggcgatg atcttgcgtt cgaaccacac catcagcatc gtgccgacga
     3361 ggccgatcac gaagatgatc agcaccttga tcgcgacgat gacgatcgac ttgccgaagt
     3421 cgatgtcgaa caggacgggg tcgagggcca gcatcacagg gtctcgatcc gaacgtcggt
     3481 gaccgccgcg ctgaggtcga tgagatcgcc aacgcgcttg ggaccgggct ggttgaacgg
     3541 cacccaggcc gtgccgcgct gcacaccggg gtcggtgtgc aaggccagca ccaccatcgc
     3601 cttggggctg gtgaccttga cctcgctgcc gaccggggcg ccgatgcggg ccacgtcgag
     3661 cgggttgacg
//
LOCUS       DAORNF010000296         3670 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_18985_length_3670_cov_1.406916, whole genome shotgun sequence.
ACCESSION   DAORNF010000296 DAORNF010000000
VERSION     DAORNF010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3670
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18985_length_3670_cov_1.406916"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            25..>253
                     /locus_tag="PLV68_12035"
     CDS             25..>253
                     /locus_tag="PLV68_12035"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRE02021.1"
                     /translation="MLVAICQFRREPLTYPEGSTIALWILGFLSMGVYMILFLEGMAR
                     TTAAEGAIILATSPVLTALLAVAMKHERFSWG"
     assembly_gap    254..384
                     /estimated_length=131
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <385..1316
                     /locus_tag="PLV68_12040"
     CDS             <385..1316
                     /locus_tag="PLV68_12040"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YhfC family glutamic-type intramembrane
                     protease"
                     /protein_id="HRE02022.1"
                     /translation="VQTRFFLTDMSPLRALTLSMPGAIPVLLVYGLAATFAVPWSQIT
                     WVGWVMLAHITLLAGVVGFLGFFVGVRQVGSSGAMLYQFLVPPIAWVVLTRRLGVSWR
                     MVGLGCLTWLTAMPFIIGVPMAATAVFGPQPLVWPVALSLTAGIVEETSRFLYYRRSA
                     ALRDRGNWRGALVAGAAHGGVESIVLGLQTAAGVVAFFFMRDWLPAEMQAIQPEPAYF
                     LIGAGSRLLIMIGHVGFTFLVWRAVSRAGEGWLYPLAIVLHIAIDLIAFAQPIVLPGA
                     DWMGFVAVIALAAGSLWLIRQSRSQHQGIAMAV"
     gene            complement(1415..>1720)
                     /locus_tag="PLV68_12045"
     CDS             complement(1415..>1720)
                     /locus_tag="PLV68_12045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02023.1"
                     /translation="QLAVYAAQRAMDQAVTVIVINKTGNALSTALNLSGFSAASGARV
                     FRYSSADLTRIVRQTDLVVTTGPLLIDAPANSITLIELKPGTPLVLTPRVRMPMLAR"
     assembly_gap    1721..1736
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1737..>2635)
                     /locus_tag="PLV68_12050"
     CDS             complement(<1737..>2635)
                     /locus_tag="PLV68_12050"
                     /inference="COORDINATES: protein motif:HMM:NF024295.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 44 protein"
                     /protein_id="HRE02024.1"
                     /translation="QQSVDPWDTNCGNGVTPGGGTITGNSPTDTSVAISSTFVRDWVL
                     HLTSTFGAANAGGVRFYNLDNEPMLWNSTHRDIHPAAATYDELRTKTLDIAAAVKQAD
                     PTARTLGPVLWGYCAYFFSARDDCAPGQDYSSHGNTYFVPWYLSQLRAYEQTNGTRLL
                     DYLDLHYYPQASGVALSGAGGATTQALRLRSTRSLWDPTYVDESWIPDLNIDGGVIRL
                     IPRMRDWVDTYYAGTGLAITEYNWGGHEHINGALAQADVLGIFGREGLDLATLWDPPA
                     STEPAAFAFRMFLNYDGVGSKFG"
     assembly_gap    2636..2710
                     /estimated_length=75
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2711..3567)
                     /locus_tag="PLV68_12055"
     CDS             complement(<2711..3567)
                     /locus_tag="PLV68_12055"
                     /inference="COORDINATES: protein motif:HMM:NF024295.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 44 protein"
                     /protein_id="HRE02025.1"
                     /translation="MPIKRWVVGALLLGLLLAPLSVSNTASAAVDRVIYADALGSGVQ
                     NWSWGTTVNAANPSPVQAGSASLAVTYTDAWAGLYLHLDAPQSTAGYDRIRFYIRGGS
                     TTAQLRFVVDGTQAGGTISAPPGTWTMIEATLGALGSPGTIQDLQWQDTTGGAQTTYY
                     LDSITLVASTGSPTATPQPSGGPTLSVNVGADRAPISRWIYGMNYPDEALAEELALPV
                     ARWGGNATTRYNWQLDVYNTGSDWYFENIPNPVANVGALPAGSAADQFVAANQAAGSD
                     TLLTVPLIG"
BASE COUNT          493 a         1052 c         1241 g          662 t
ORIGIN      
        1 gttcggttcc ttctcatgtg gggggtgctg gtggccatct gccagtttcg ccgcgagccc
       61 ctgacctatc ccgaaggctc gaccatcgcc ttgtggattc tcgggtttct ctcgatgggt
      121 gtttatatga tcctcttcct cgaagggatg gcccggacca ccgccgcgga gggagcgatc
      181 atcctggcga cgtcgccggt tctcaccgca ctcctcgcgg tcgcgatgaa acacgagcgc
      241 ttcagttggg gcgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnccgtgc agactcgatt tttcctgacc gatatgtcgc
      421 ccctaagggc cctgactctc tcgatgcccg gtgcgatccc ggtcctgctc gtctacggcc
      481 ttgccgccac tttcgctgtt ccatggtcac agatcacctg ggtggggtgg gtcatgctcg
      541 cccacatcac cctcctcgcc ggcgtggtcg ggttcttggg cttcttcgtg ggcgtgcgcc
      601 aagtcgggag cagtggcgcg atgctctatc agtttctggt tccgccgatc gcctgggtcg
      661 tgttgacccg taggctcggc gtctcatggc ggatggtcgg gctcggctgt ttgacctggc
      721 tgaccgcgat gccgttcatc atcggcgttc cgatggcggc gacggcggtg tttggtccgc
      781 aaccgctcgt ctggcccgtg gcgctctcgc tgacggccgg catcgtcgag gagacttcgc
      841 ggttcctgta ttaccggcgc agtgctgccc tgcgtgaccg gggcaactgg cggggcgcgt
      901 tggtggccgg cgcggcacat ggcggggtcg agtcgatcgt gctcggcctg cagacggcgg
      961 ccggtgtcgt cgcgttcttc ttcatgcgcg attggctgcc agccgagatg caggcgatcc
     1021 agcccgagcc cgcctacttt ttgatcggcg ccggctcgcg gctgttgatc atgatcgggc
     1081 atgtcggctt cacattcctg gtctggcgcg cggtgagccg tgccggcgag ggctggctgt
     1141 atccgctggc gatcgtcctc cacatcgcca tcgacctgat cgcgttcgca cagccgatcg
     1201 tgctgccggg cgccgactgg atgggttttg ttgccgtcat cgcattggcc gcgggtagcc
     1261 tctggctgat ccgacagagc cgttcgcagc atcaggggat cgcgatggcc gtgtgagagg
     1321 tgggaggagc atggagcgtg gagcgtggag cgaggtgcgt ggagcggggt gaggggtgcg
     1381 actcgcatcc ctcaccccgc tcttcgttcc acacctaacg cgccagcatc ggcatacgga
     1441 cgcgtggggt gagcaccagc ggcgtgccgg gcttgagctc gatgagcgtg atcgagttgg
     1501 ccggggcatc gatgagcagc gggccggtgg tgacgaccag gtcggtctgg cgcacaatac
     1561 gcgtcagatc ggctgacgag tagcggaaga cacgggcacc cgacgcggcc gagaagcccg
     1621 acaggttgag cgcggtggat aacgcgttgc ctgtcttgtt gatcacgatg accgtcaccg
     1681 cctgatccat ggcccgttgg gcggcgtaga ccgcgagctg nnnnnnnnnn nnnnnntcgc
     1741 cgaacttgga gccgacaccg tcatagttga ggaacatgcg gaaggcgaag gctgccggct
     1801 cggtcgacgc gggcggatcc cacaacgtgg cgaggtcgag gccctcgcgg ccgaagatgc
     1861 cgagcacgtc ggcctgggcg agcgcgccat tgatgtgctc gtggccgccc cagttgtact
     1921 ccgtgatcgc aaggccggtg ccggcgtagt aggtgtcgac ccaatcgcgc atgcgcggga
     1981 tcaaacggat cacgccgccg tcgatgttga ggtccgggat ccagctttcg tccacgtagg
     2041 tcggatccca cagcgaacgc gtgctgcgca gccgcagggc ctgtgtggtc gcgccgccgg
     2101 cgccgctgag cgcgacgccg ctggcttgcg ggtagtagtg cagatcgagg tagtcgagca
     2161 gacgcgtgcc gttggtctgt tcgtaagcgc gcagctggct gaggtaccag ggcacgaagt
     2221 aggtgttgcc gtgcgagctg tagtcctggc ccggcgcgca gtcgtcgcgc gccgaaaaga
     2281 agtaggcgca gtagccccac agcaccggcc cgagcgtccg cgcggtcggg tccgcctgct
     2341 tgacggcggc ggcgatgtca agggtcttcg tgcggagctc atcgtaggtc gctgccgccg
     2401 ggtggatgtc gcgatgggtg ctgttccaca gcatgggctc gttatcaagg ttgtagaagc
     2461 gcacgccgcc ggcgttggcg gcgccgaacg tcgatgtgag atgcaacacc cagtcgcgca
     2521 cgaacgtcga cgagatcgcg acgctggtgt cggtcgggct attgcctgtg attgtgccac
     2581 cgcctggcgt cacgccgttt ccgcagttgg tatcccacgg gtcgacgctc tgctgnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn cagccgatga gcgggacggt cagcagcgtg tcgctgccgg cggcctggtt
     2761 cgccgccacg aactgatcgg cggccgagcc ggcgggcagg gcgccgacgt tggctaccgg
     2821 gttggggatg ttttcgaagt accagtcgct gccggtgttg tagacgtcga gctgccagtt
     2881 gtagcgcgtc gtcgcgttgc cgccccagcg cgccaccggg agcgccagct cttcggcgag
     2941 tgcctcatcg ggatagttca tgccgtagat ccagcggctg atcggcgcgc ggtctgcgcc
     3001 tacgttgacg gaaagcgtcg gaccgccgga gggctgtggc gtggcggtcg ggctgccggt
     3061 gctcgcgacc agcgtgatgc tgtcgaggta gtacgtcgtt tgggcgccgc cggtcgtgtc
     3121 ttgccactgc agatcctgga tcgtgccggg cgatccgagc gcgcccaacg tcgcctcgat
     3181 catcgtccac gtcccgggcg gggccgagat cgtgccgccc gcttgggtgc cgtcgaccac
     3241 gaaacggagt tgggccgtcg tcgatccgcc gcggatgtag aagcgaatgc gatcatagcc
     3301 ggctgtgctc tgcggcgcgt cgaggtgcag atagagcccg gcccaggcgt cggtgtaggt
     3361 gaccgccagc gaagccgagc ccgcctggac cggggagggg ttagcggcat tgaccgtggt
     3421 tccccacgac cagttctgca cgccgcttcc gagcgcgtcg gcgtagatca cgcggtcgac
     3481 ggctgcgctc gcggtgttcg atacgctcaa cggcgcgagc aacagaccca gcaagagcgc
     3541 gccaacgacc caacgtttga tcggcatagt ccctccgcag tgattgtaga ggcgacgggc
     3601 ccgtgttggt gcccggaaaa ggggcgggaa ggggtcggta attggtctca atggcggggg
     3661 gagacgactg
//
LOCUS       DAORNF010000297         3651 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_19094_length_3651_cov_1.492770, whole genome shotgun sequence.
ACCESSION   DAORNF010000297 DAORNF010000000
VERSION     DAORNF010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3651
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19094_length_3651_cov_1.492770"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>656
                     /locus_tag="PLV68_12060"
     CDS             <1..>656
                     /locus_tag="PLV68_12060"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442137.1"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine dehydrogenase
                     (aminomethyl-transferring)"
                     /protein_id="HRE02026.1"
                     /translation="EGVILQTNPSTCAAASVANVARHFGIDATEATAAAIDASSPPDG
                     LPVARRRTDAFLTHSVFNRYHSEHEMLRYLRRLADKDLALDRTMIPLGSCTMKLNATV
                     EMMPITWRGFTEIHPFAPPTDTVGYREMIAQLERMLAAITGYDAVSLQPNAGSQGEFA
                     GLVAIRLYHESRGEPGRVVCLIPASAHGTNAASAVMAGLQVVVVACDDHGNVDVGDLA
                     A"
     assembly_gap    657..791
                     /estimated_length=135
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <792..1611
                     /locus_tag="PLV68_12065"
     CDS             <792..1611
                     /locus_tag="PLV68_12065"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379186.1"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycine dehydrogenase
                     (aminomethyl-transferring)"
                     /protein_id="HRE02027.1"
                     /translation="VGLAQPGRFGADVSHLNLHKTFCIPHGGGGPGVGPVAVRSHLAQ
                     FLPGDPTTEDPIRKGGAVSAAEFGSASILPISWMYIRLMGDAGLLAATEAAILNANYV
                     AARLDAAFPVLYRGERGYVGHECILDLRPITKATGVTVDDVAKRLMDYGFHAPTMSFP
                     VAGTLMVEPTESETLGELDRFCDAMLAIRAEIARIESDEWAVADSPLRHAPHTAEDLL
                     GDWDRVYPRDLGAYPVDGLRAAKYFPPVSRIDAAYGDRHLACSCAPLEAYADAR"
     gene            1832..>2159
                     /locus_tag="PLV68_12070"
     CDS             1832..>2159
                     /locus_tag="PLV68_12070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02028.1"
                     /translation="MSTSSTAAPATTTTVAESVPPTDTAVPTTDSTPPTEPPPPPSTN
                     PLPPGWQPIDFWPASGVQGYSGNWCCTASPPAPASPDQSPADGYYPATYLTPWQPGDT
                     AVHLQVQ"
     assembly_gap    2160..2217
                     /estimated_length=58
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2218..2545
                     /locus_tag="PLV68_12075"
     CDS             <2218..2545
                     /locus_tag="PLV68_12075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02029.1"
                     /translation="LEQGIDPAWSLDLNVPLDTTSRVVVRGFACGGDDNGDDENAAKV
                     ASGFELAELFEAYNADYNQYVVPRLADYPNGWEATQAIAADAAAGAAGVRAAGARRRP
                     GGPGGA"
     assembly_gap    2556..2609
                     /estimated_length=54
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2838..2869
                     /estimated_length=32
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2870..>3246
                     /locus_tag="PLV68_12080"
     CDS             <2870..>3246
                     /locus_tag="PLV68_12080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02030.1"
                     /translation="QMRKGVNEFMTAVENFNQSMATINQIAQRANALLDDVEAPIRAM
                     LPQVTRSIKAADAVIDQITGPIERVAPGISRLADTLANPIFTSMPHDIAGFLDSFGEI
                     AKRMQPLAQMAESAGSMFGFRNL"
     assembly_gap    3247..3333
                     /estimated_length=87
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          536 a         1216 c         1063 g          470 t
ORIGIN      
        1 gaaggtgtca ttctccagac caatccgtct acctgcgccg cggcctccgt cgccaatgtc
       61 gcgcgccact tcggcatcga cgccaccgag gccacggccg ccgcgatcga cgcgtcgtca
      121 ccgcccgacg gtctgcctgt ggcgcggcgg cgcaccgatg cgttcctcac ccacagcgtg
      181 ttcaaccgct accacagcga acacgagatg ctgcgctatc tgcgccgtct ggccgacaag
      241 gacctagccc tcgaccgcac gatgatcccg ctcggttcgt gcaccatgaa gctcaacgcg
      301 acggtcgaga tgatgccgat cacgtggcgt ggcttcaccg agatccatcc gttcgcgcca
      361 ccgaccgaca ccgtcggcta ccgcgagatg atcgcccagt tggagcggat gctcgccgcg
      421 atcaccggct acgacgcggt gagcctgcaa cccaacgcag gcagccaggg cgagttcgcc
      481 gggctggtcg cgatccggct ctatcacgag agccgcggcg agcccggtcg ggtcgtgtgc
      541 ctcatcccgg ccagcgccca cggcaccaat gcagccagcg ccgtcatggc cgggctacag
      601 gtggtggtcg tggcctgtga cgatcacggc aacgtcgacg tcggcgatct ggcggcnnnn
      661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn nggtcggtct ggcccagccc ggccgcttcg gtgccgacgt cagccacctc
      841 aacctgcaca agacgttctg catcccccac ggcggtggtg gaccgggcgt cgggccggtc
      901 gcggtccgct cccacctggc ccagttcctc cccggcgacc cgacgaccga agacccgatc
      961 cgcaagggcg gcgcggtgtc ggctgccgag ttcgggtcgg ccagcatcct gccgatctcg
     1021 tggatgtaca tccgcctgat gggtgacgct ggcctgctcg ccgccaccga ggccgcgatc
     1081 ctcaacgcca actacgtcgc agcccgcctc gatgcagcct ttcccgtgct gtaccgcggc
     1141 gagcgcggtt acgtcggcca cgagtgcatc ctcgacctgc gtccgatcac caaggccacg
     1201 ggtgtcaccg tcgacgacgt ggccaagcgc ctgatggact acggcttcca cgcgccgacg
     1261 atgagcttcc ccgtcgcggg cacgctgatg gtcgagccga ccgagagcga gacgctgggc
     1321 gagctcgacc gcttctgcga cgcgatgttg gcgatcaggg ccgagatcgc ccgcatcgaa
     1381 agcgacgagt gggccgtcgc cgacagcccg ctgcgtcacg ccccgcacac cgccgaggac
     1441 ctgctcggcg actgggaccg ggtgtacccg cgcgacctgg gcgcgtaccc cgtcgatggc
     1501 ctgcgcgcgg cgaagtactt cccgcccgtg tcgcgtatcg acgcggccta cggcgaccgt
     1561 catctcgcgt gttcgtgcgc cccgttggag gcatacgctg acgctcgatg actgtgactc
     1621 gctggcccgc ccgtcgaacc ttgcgtcgaa cgctcctggc ggtcgcgacc ggggcgtgca
     1681 tgatcctggc cgcgtgcggc agcggtggcg gcccagcgcc aggtaggcga acggaccgac
     1741 cggctgcacg aagcagccga gggcccacag ggccttgctg ccgcgcacct gcgatgctgg
     1801 ccggcgggcc aggtcgacca gcgccgtggt ggtgagcacc agctcgaccg ctgcgccggc
     1861 cacgaccacg acggtggccg agtcggtgcc accgaccgac actgcggtgc ccaccaccga
     1921 ctcgacaccg cccaccgagc cgccaccgcc gccgtccacc aacccgctcc cgcccggttg
     1981 gcagccgatc gatttctggc ccgcgtctgg cgtgcagggc tactcgggca actggtgctg
     2041 caccgcttca ccacccgcgc ctgccagccc cgatcagtcg ccggccgatg gctactaccc
     2101 cgccacctac ctgactccgt ggcagcccgg tgacaccgcc gtgcacctgc aggtacagcn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngtt
     2221 ggagcagggc atcgacccgg catggtcgct cgacctgaac gtcccgctcg acaccacctc
     2281 cagggtcgtc gtgcgcggat tcgcctgcgg tggtgacgac aacggcgacg acgagaacgc
     2341 tgccaaggtc gcgtccggct tcgagttggc cgagctgttc gaggcctaca acgccgacta
     2401 caaccagtac gtcgtgcctc gcttggccga ctaccccaac ggatgggagg ccacccaggc
     2461 gatcgcagcc gacgcggcgg ctggtgccgc tggtgtccgg gctgcaggag cccgccgccg
     2521 accaggcgga ccaggtggcg cctgaatccg acgagnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc ccctgccgat caacgcgttg atcaccctgg
     2641 agggagtgca gtacacggcc ggagtgccca ccttgtactt ctacgccggg ttctactcct
     2701 gacggccacg cttgccagac ggtgcctcct ggcgccgcgg cgctgctgca tcgcgccagc
     2761 gacggctact gttccgccca tggcagaacc ttcatcgacg cccggcttcg gcgacttgct
     2821 caacctggtc ggcaacgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc cagatgcgca
     2881 agggtgtcaa cgagttcatg acagcggtcg agaacttcaa ccagtcgatg gccaccatca
     2941 accagatcgc ccagcgcgcc aacgcgctgc tcgacgacgt cgaggcgccg atccgggcga
     3001 tgctgcccca ggtgacgcgc agcatcaagg ccgccgacgc ggtgatcgac cagatcaccg
     3061 gcccgatcga gcgagtcgcc cccggcatca gccgcctggc cgacaccctg gccaacccga
     3121 tcttcacctc gatgccccac gacatcgccg ggttcctcga cagcttcggc gagatcgcca
     3181 agcggatgca gccgctggca cagatggccg agtcggcagg cagcatgttc gggttccgca
     3241 acttgcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnaaagaag gctcccgcca agaaggctcc
     3361 cgccaagcca accccggcca aagccgctgc ggcgaaacga gtgaccacca ccaagaccgg
     3421 tacggccgcc aagaaggcac cggccaagaa gtccagcgcg cgacgctgac aaccggccgg
     3481 ctggcggcca accgtcgctg gacgacgcgg gtcagcgggt gacgaacggg gtcggcacga
     3541 cgcggccggg caggtggctg ccgcgcacgt cgatgctgac gtcggtgccg gggtcggtac
     3601 cggtcggcat gaacgccagg gcgatgccgt gctccagcac tggcgagaag t
//
LOCUS       DAORNF010000298         3646 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_19130_length_3646_cov_2.111947, whole genome shotgun sequence.
ACCESSION   DAORNF010000298 DAORNF010000000
VERSION     DAORNF010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3646
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19130_length_3646_cov_2.111947"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..979
                     /gene="proB"
                     /locus_tag="PLV68_12085"
     CDS             <1..979
                     /gene="proB"
                     /locus_tag="PLV68_12085"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HRE02031.1"
                     /translation="GHEVILVSSAAISAGVAALGLSARPTDVLTLQALAAAGQPRLME
                     EYNRALGRHGIVAAQVLLVPHDFADRQQYLHSRDTLTRLIEIGCIPIVNENDTVANDE
                     IRFGDNDRIAALVAHNVSADLLVLLTDTPGLYTADPRIDPSAALIVEVAANDPLLSVS
                     AGAQGTARGSGGMASKLQAARIASWSGVRAVIAKATREHALVDAARGIPVGTSFAPND
                     RLSARKLWIGFASHSQGRVMVDNGAYRALVSAGSSLLPAGVVAVEGDFSEGATVEVCT
                     ADGTVFARGMTVLDAANTQQYMGRSTRDLPARIAHEVIHRDDLVVLPHD"
     assembly_gap    1002..1046
                     /estimated_length=45
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1055..1846)
                     /locus_tag="PLV68_12090"
     CDS             complement(1055..1846)
                     /locus_tag="PLV68_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HRE02032.1"
                     /translation="MFKVFRKWWKYLGAKFNKNFDDRADPAIQLEQALNEAQGQHQRL
                     KEQAVNVIATQKQTELRLNAKLAELEKLNGNARQALVMAADAAKAGDTAKANQYNAAA
                     ETIAGQLITVEKDAEGLKQMVMDATQAADQAKAAVAQNGRLLQQKLAEKNKLLSQLEQ
                     AKMQEQMSKAMDQLNETVGSDVPSLQEVSNKIEARYAKAKAASELGETSVTSRIHEIE
                     MATANVEAQGRLSQLREELGLNPAPAAAPTPEQLTGGQEQAQPSA"
     gene            complement(1891..3459)
                     /gene="murJ"
                     /locus_tag="PLV68_12095"
     CDS             complement(1891..3459)
                     /gene="murJ"
                     /locus_tag="PLV68_12095"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HRE02033.1"
                     /translation="MLRNNIVVAIGTALSRVTGMLRVAVLGLVIGPRAVADAYNAANS
                     SPNTIYELLLGGALSDSLVPLFSKLDEDKDDEGTVAVVSTALVAMIALTVVAVVAAPW
                     IFHLYSLNPSDNVDAEQFRRVGTLLARIFLIQIFFYGLTSLGTSMLNARRRFFAAAWS
                     PVLSNLIIIASLLMIPHLYDGDVTLAAVLEHRSLQLALGLGATLGIAAMALSLYPALR
                     RSGAPLWFSTDFRHPAVKKLLQLSGWTLGYAAANQVAVVVIQNLALNQGEGRQDLYIK
                     AFTFFVLPHGLLAVSIATTFEPEMARSVAARNKPAFIDRTSLGVRMVALLTIPAGFGL
                     FVLRRPIIGVALQHGKFTAADALEASRALAGLALGLVGFSVYLFTLRAFYSHQDARTP
                     FVINIFENLINIVLAIVLVPRYGILGLGLALAIAYLVSAAWALLVLSYKVSGFELRTV
                     YRGLWPMAVAGLVMAEAVWFVTRHVGANSGLDGIVRVGVGAVVGIGVYVAMLVVLGVP
                     ELATLRERLPGRRR"
BASE COUNT          558 a         1187 c         1258 g          598 t
ORIGIN      
        1 gggccacgag gtgatcctgg tcagttcggc cgcgatctcc gccggggtcg ccgcgctcgg
       61 tctgtccgcc cggccgaccg acgtgttgac gttgcaggca ctggccgctg ccggtcagcc
      121 gcggttgatg gaggagtaca accgtgccct cggccgccac ggcatcgtcg ctgctcaagt
      181 gctgctggtg ccgcacgatt tcgccgaccg ccagcagtac ctgcactcgc gcgacacgct
      241 cacccggttg atcgagatcg gctgtatccc gatcgtcaac gagaacgaca cggtggccaa
      301 cgacgagatc cgtttcggtg acaacgaccg catcgccgcc ttggtcgcgc acaacgtcag
      361 cgccgaccta ctcgtgctgt tgaccgacac gcccggcctg tacaccgccg atccgcgcat
      421 cgatccgtcg gcggccctga tcgtcgaggt cgccgccaac gatccgctgc tatcggtcag
      481 cgcgggtgcg cagggtacgg cacgggggag cggcggcatg gcgtccaagc tgcaggcggc
      541 ccgcatcgcc agttggtcgg gggtacgcgc cgtgatcgcc aaggccacgc gcgagcacgc
      601 gctggtcgac gccgcccgcg gcattccggt gggcacgtcg ttcgcgccca acgatcggct
      661 gtcggcacgc aagctctgga tcggcttcgc cagccacagc caaggacgag tcatggtcga
      721 caacggcgcc taccgcgccc tggtcagcgc cggcagctcg ttgctgcccg ctggtgtcgt
      781 cgcggtcgag ggtgacttca gcgagggtgc cacggtcgag gtgtgcaccg ccgacgggac
      841 cgtcttcgcc cgtggcatga cggtgctcga cgcggcgaac acgcagcagt acatgggccg
      901 cagcacgcgc gatctgccgg cccgcatcgc ccacgaggtc atccaccgcg acgacctcgt
      961 ggtgctgccc cacgactgac cacacgcgca acgaccggct cnnnnnnnnn nnnnnnnnnn
     1021 nnnnnnnnnn nnnnnnnnnn nnnnnngccg gccgtcacgc gctgggttgg gcctgttctt
     1081 gcccgcccgt cagttgctcg ggcgtgggcg cagcggcggg agccgggttg aggccaagct
     1141 cttcacgcaa ctggctgagg cggccctgcg cttcgacgtt ggcggtggcc atctcgatct
     1201 cgtggatgcg gctggtgacc gacgtctcgc cgagctcgct ggcggccttg gccttggcgt
     1261 agcgggcctc gatcttgttg ctgacctctt gcaggctggg cacgtcggag ccgacggtct
     1321 cgttgagctg gtccatggcc ttgctcatct gctcttgcat cttggcctgc tccaactggc
     1381 tgagcagctt gttcttctcg gccagcttct gctggagcag acgaccgttc tgggcgaccg
     1441 cggccttggc ctgatcggca gcctgggtgg cgtccatcac catctgcttc aggccctcgg
     1501 cgtccttctc gacggtgatc aattgccccg cgatggtctc ggccgcggcg ttgtactggt
     1561 tggccttggc ggtgtcaccg gccttggccg cgtcggcggc catcaccagc gcctgacggg
     1621 cgttgccgtt gagcttctcg agttcggcca acttggcatt gaggcgcagc tcggtctgct
     1681 tctgggtggc gatgacgttg accgcctgct ccttcaaccg ctggtgctgg ccctgggcct
     1741 cgttgagcgc ctgttcgagt tggatcgcgg ggtcggcccg atcgtcgaag ttcttgttga
     1801 acttggcccc gaggtacttc caccacttgc gaaacacttt gaacatggcg gcgagcctaa
     1861 cgcctcagca ggtgtggcga ttgcagtgtg ttaccggcgc cgaccgggga ggcgttcgcg
     1921 caaggtggcc agttcgggca cgccgagcac gaccagcatc gcgacgtaga caccgatgcc
     1981 gacgaccgcg ccgacaccga cccgaacgat gccgtcgagg ccgctgttgg cgccgacgtg
     2041 gcgggtgacg aaccacaccg cttcggccat caccaaaccg gcgaccgcca tcggccacag
     2101 acctcggtac accgtgcgca gttcgaagcc cgacaccttg tagctcagca ccagcagcgc
     2161 ccaggccgcg ctgacgagat aggcgatcgc caaggcgaga ccgagaccga ggatcccgta
     2221 ccgcggcacg agcacgatcg cgagcacgat gttgatcagg ttctcgaaga tgttgatcac
     2281 gaacggggtg cgcgcgtcct ggtgggagta gaaggcgcgc agcgtgaaca ggtacacgct
     2341 gaagccgacc agcccgagcg ccagcccggc gagggcgcgg ctggcttcca gcgcgtcggc
     2401 ggcggtgaac ttgccgtgtt gcagcgcgac gccgatgatc gggcggcgca gcacgaacag
     2461 tccgaacccg gcggggatcg tcaacagcgc gaccatccgc acgccgagcg aagtgcggtc
     2521 gatgaacgcg ggcttgttgc gggccgcgac cgagcgcgcc atctccggct cgaacgtggt
     2581 cgcgatcgac accgcgagca agccgtgggg cagcacgaag aacgtgaacg ccttgatgta
     2641 caggtcttgg cggccctcgc cttggttgag cgcgaggttc tggatgacga cgacggcgac
     2701 ctgattggcg gccgcgtagc ccagcgtcca accggagagt tggagcagct tcttgaccgc
     2761 ggggtggcgg aagtcggtcg agaaccacag cggcgcgccc gatcggcgca gcgcggggta
     2821 gagcgacaac gccatcgcgg cgatgccgag cgtggccccc aggccgagcg cgagttgcag
     2881 gctgcggtgt tccaacaccg cagccagggt cacgtcgccg tcgtagagat gagggatcat
     2941 cagcaggctg gcgatgatga tcaggttcga cagcacgggt gaccacgcgg cggcgaaaaa
     3001 gcgtcggcgg gcgttgagca tcgaggtgcc cagcgaggtg aggccgtaga agaagatctg
     3061 gatcaggaag atgcgcgcca gcagcgtgcc gacccggcgg aactgctcgg cgtcgacgtt
     3121 gtcggacggg ttgagcgagt acaggtggaa gatccacggc gcagcgacga cggcgacgac
     3181 ggtcaacgcg atcatcgcta ccagcgcggt gctgacgacg gcgaccgtgc cctcgtcgtc
     3241 cttgtcctcg tccagtttgc tgaacagcgg caccaggctg tccgacagcg ccccgccgag
     3301 cagcagttcg tagatcgtgt tggggctgct gttggccgcg ttgtaggcgt cggccaccgc
     3361 gcgggggccg atcaccaggc cgaggaccgc gacgcgcagc atgccggtca cgcgtgagag
     3421 tgcggtgccg atcgcgacga cgatgttgtt gcgcagcatc ccactcgctg gccggacgcc
     3481 ggcgggtgcc accgtggcgg tcacgcggtc cctggctgct cggtgccgga ctgcggggta
     3541 cctggcgggt cggtatgccg cggttgctcg acgtaacccg gttcgacgtg acccggttcg
     3601 atgcggcccg gcccggtctg aaccgtgagg ccgccgtctt ccatcg
//
LOCUS       DAORNF010000299         3646 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_19135_length_3646_cov_1.468115, whole genome shotgun sequence.
ACCESSION   DAORNF010000299 DAORNF010000000
VERSION     DAORNF010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3646
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19135_length_3646_cov_1.468115"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>252
                     /locus_tag="PLV68_12100"
     CDS             <1..>252
                     /locus_tag="PLV68_12100"
                     /inference="COORDINATES: protein motif:HMM:NF015648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HRE02034.1"
                     /translation="AASIIAGIAVLVALDDGGLKTFLKWVTLIAIVLTAIWTVFRYLK
                     WITTNFVITSDRLIFRHGVVGKMGIEIPLERVNNVNFHQT"
     assembly_gap    253..349
                     /estimated_length=97
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <350..537
                     /locus_tag="PLV68_12105"
     CDS             <350..537
                     /locus_tag="PLV68_12105"
                     /inference="COORDINATES: protein motif:HMM:NF021378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HRE02035.1"
                     /translation="QNLVHAQVENKSRQRGGYAAAAYGSVGADVATQLEKLEGLFQRG
                     SLTEDEFEAQKRRLLDG"
     assembly_gap    685..727
                     /estimated_length=43
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    940..1048
                     /estimated_length=109
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1049..1879)
                     /locus_tag="PLV68_12110"
     CDS             complement(<1049..1879)
                     /locus_tag="PLV68_12110"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03560 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HRE02036.1"
                     /translation="MTIFGVHTGLQHISYADLRGSWRRIEEFGFGWISIWDHFYRATG
                     RPDDAMCFEAVAMHAALAAETSRVTVGALVYSIGYRHPAVLAKAITAIDHISGGRAAM
                     GMGSGWAETEYLAYGIDFPPPKVRLDQLEEGIQIVRGLLHDEVTTCAGEYFSATDARN
                     EPRPVQAKMPIWVGGAGEKRTLSIAARFADGWNIPFVAPEVFAHKRGVLHEHCERVGR
                     DPGEITCAVNVGLAFSEESLHAQFGELASWVGPGVLTGSDEQVIDRVGQFIDAGADQV
                     N"
     assembly_gap    2154..2172
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2173..>2535
                     /locus_tag="PLV68_12115"
     CDS             <2173..>2535
                     /locus_tag="PLV68_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRE02037.1"
                     /translation="IRFDNRDCGLSTKLDGVAVDVAGVMAAVAAMMAGGTAELPPVPY
                     TLSDMAADAVGLLDHLGIERAHIVGASMGGMIAQTVAIEHASRTATLVSIMSTPGDPD
                     VTAPAPEAAAVLFSVPPPD"
     assembly_gap    2536..2560
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2561..2912
                     /locus_tag="PLV68_12120"
     CDS             <2561..2912
                     /locus_tag="PLV68_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRE02038.1"
                     /translation="VASKVWRSKRYTDLDVVRAEAGRAYDRSFYPEGAMRQMAAIQAS
                     GSRSAALRDLDVATLVVHGRDDTLIPPAAGFRTAELIPGSVLLFVSDMGHDIPEPLWS
                     TVTDAIVSHTNPHR"
     gene            2985..>3305
                     /locus_tag="PLV68_12125"
     CDS             2985..>3305
                     /locus_tag="PLV68_12125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRE02039.1"
                     /translation="MAGPLSGYRIIEIAGIGPGPFAAMLLSDMGAEVIRVERSQAVRP
                     NAAPNGDVLQRNRRNIAIDLKSEAGRDTLLQLVERADALIEGFRPGVMERLGVGPDEC
                     LARNP"
     assembly_gap    3306..3362
                     /estimated_length=57
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3363..>3646
                     /locus_tag="PLV68_12130"
     CDS             <3363..>3646
                     /locus_tag="PLV68_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRE02040.1"
                     /translation="HDINYISLAGSLAHFARAGQMPQPPLNMIGDFGGGGMFLAYGVV
                     CALLEAAKSGQGQVVDTAMVDGTAVLMTMFWAFKNQGIFDENAPGTNLLD"
BASE COUNT          546 a         1144 c         1057 g          549 t
ORIGIN      
        1 ggcggcctcg atcatcgccg gcatcgcggt gttggtcgcg ctcgacgacg gtggcctcaa
       61 gacgttcctc aagtgggtca cgctgatcgc gatcgtgctc acggcgatct ggaccgtctt
      121 ccgctatctc aagtggatca ccaccaactt cgtcatcacc agcgaccggc tcatcttccg
      181 ccacggcgtc gtcggcaaga tgggcatcga gatcccgctc gaacgtgtca acaacgtcaa
      241 cttccaccag acnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt gcagaacctg
      361 gtccacgccc aggtcgagaa caagtctcgt cagcgcggtg ggtacgccgc tgcggcgtac
      421 ggatccgtcg gcgcggacgt cgcgacccag ttggaaaagc tcgagggtct gtttcagcgt
      481 ggttcgctga ccgaggacga gttcgaagcc cagaagcgcc ggttgctcga cggctgaccc
      541 gaccactacg tcgaccagta cgtcgtctcg gtacctcgtc agcggtcgcc tcggccgatc
      601 cggtcgagcg acaccaccgt gccatagccc gtgaagcgac cgtcgctgtc gcgctcgatc
      661 gtctgcatcg cgatgatggc gcatnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      721 nnnnnnngcg ccgggatggg cgggcgcctc ccacacgcat ggcacgctga gcgcgccggt
      781 gtcgacgtgc agcaccacga actcaccggg gacctgggtg tagaacgcat tggccaccgt
      841 gagcagcacg tcgtgcgtac cggtgcaatg gatgaatcct tccgtttgca acgatggtgc
      901 ggtcacgggc tcatctggac cgaacgcgtc ccaggcgtcn nnnnnnnnnn nnnnnnnnnn
      961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1021 nnnnnnnnnn nnnnnnnnnn nnnnnnnngt tgacctgatc ggcgcccgcg tcgatgaact
     1081 ggccaacccg gtcgatgacc tgttcgtcgc tgccggtgag tacgccaggt cccacccacg
     1141 acgcgagctc gccgaactgg gcgtgcaggc tctcttcgct gaaggccagc ccgacgttga
     1201 ccgcacacgt gatctcgcca ggatcgcgcc cgacgcgttc gcagtgctcg tgcagcacgc
     1261 cgcgcttgtg ggcgaacacc tcgggcgcga cgaagggaat gttccaccca tcggcaaagc
     1321 gggccgcgat cgacagggtg cgcttctcgc cagcgccgcc gacccagatc ggcatcttgg
     1381 cctgcacggg ccgcggctcg ttgcgggcat cggtggcgct gaagtactcg ccggcacagg
     1441 tggtgacctc gtcgtggagc aggccacgca cgatctggat gccctcctcc aactggtcga
     1501 gacgcacctt gggcgggggg aagtcgatgc cgtaggccag gtactcggtc tcggcccaac
     1561 ccgaccccat gcccattgcg gcccggccac cggagatgtg gtcgatcgcc gtgatcgcct
     1621 tggccagcac tgccgggtgg cgatagccga tcgaatagac cagtgctccg acggtgacgc
     1681 gcgacgtctc ggctgcgagt gccgcatgca tcgccaccgc ttcgaagcac atcgcgtcgt
     1741 ccggtctgcc cgtggcccta tagaagtggt cccagatcga gatccagccg aagccgaact
     1801 cttcgatgcg tcgccacgag ccacgcaggt ctgcatagga gatgtgttgc agcccggtat
     1861 gcaccccgaa gatcgtcatg cgccgacggt actgcgccgc cgtcgactgc caggcgtccg
     1921 agcacggcag gctcgcgagg ccgtgctgct ccacgttgac gggagaggtc cagccccacg
     1981 agactcggca aatgccccag gcccaggtca acaccaaccc gtccaccgac gacccgatcg
     2041 tggtcgaata cgacacattc ggctcacccg ccgaccccgc agtgctgctg atcatgggct
     2101 tcaccgctca gatgacggca tggcccgacg gcttctgtcg tgacatcgcc gacnnnnnnn
     2161 nnnnnnnnnn nnatccgctt cgacaaccgc gactgcgggt tgagcaccaa gctcgacggt
     2221 gtcgcggtcg acgtcgccgg ggtgatggcg gccgtcgcgg cgatgatggc cggtggcacc
     2281 gcggagttgc cgcccgtgcc gtacacgctc agcgacatgg ctgccgacgc ggtcggcctg
     2341 ctcgaccatc tcggtatcga gcgggcccac atcgtcggcg cgtcgatggg tggcatgatc
     2401 gcccagaccg tggcgatcga acacgccagc cgcacggcga cgctggtgtc gatcatgtcc
     2461 acacccggcg atcccgacgt caccgccccc gctcccgaag cggctgccgt gttgttctcg
     2521 gtaccgccac ccgatnnnnn nnnnnnnnnn nnnnnnnnnn ggtcgcctcc aaggtgtggc
     2581 gatcgaagcg ctacaccgat ctcgatgtgg tgcgggccga ggccggccgg gcatacgacc
     2641 gcagcttcta ccccgagggg gcgatgcgcc agatggccgc catccaggcc agcggttcac
     2701 gctccgcggc gctgcgtgat ctcgacgtcg cgactttggt cgttcacggt cgcgacgaca
     2761 ccttgatccc gcccgcggcg ggcttccgca cagccgagtt gatccccggc tcggtgttgt
     2821 tgttcgtgtc cgacatggga cacgacatcc ccgaaccgct gtggtctacg gtgaccgacg
     2881 cgatcgtgtc gcacaccaac ccccaccgct gagccggctc cacgccgttc ggccccccac
     2941 tgcgtcgtcg gcaacggcgg gcactcaccg acaggagact ccccatggca ggtccactca
     3001 gcggctatcg catcatcgag atcgccggta tcggccccgg cccgtttgcg gccatgctgc
     3061 tcagcgacat gggtgcagag gtcatccgcg tcgagcgcag ccaggcggtg cgacccaacg
     3121 ccgcccccaa cggcgacgtc ctgcaacgca accggcgcaa catcgcgatc gacctcaaga
     3181 gcgaggcggg gcgcgacacg ttgctgcagt tggtcgagcg tgctgacgcg ctgatcgaag
     3241 ggttccgccc gggtgtgatg gaacgccttg gcgtcggtcc tgacgagtgc ctggcgcgca
     3301 accccnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnggcacgac atcaactaca tctctctcgc cgggtcgctc gcccacttcg cccgcgctgg
     3421 gcagatgccc cagccgccgc tcaacatgat cggcgacttc ggtggcggtg gcatgttctt
     3481 ggcctacggc gtggtctgcg ccctgctgga ggcggccaag agcggtcagg gccaggtggt
     3541 cgacacggcc atggtcgacg gtaccgcggt gttgatgacc atgttctggg cgttcaagaa
     3601 ccaaggcatc ttcgacgaga acgcgccggg caccaacctg ctcgac
//
LOCUS       DAORNF010000300         3646 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_19137_length_3646_cov_1.397104, whole genome shotgun sequence.
ACCESSION   DAORNF010000300 DAORNF010000000
VERSION     DAORNF010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3646
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19137_length_3646_cov_1.397104"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="PLV68_12135"
     CDS             <1..294
                     /locus_tag="PLV68_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRE02041.1"
                     /translation="CIYIEGHKDLAPPRRAGGAPRKVNKLDRFDIDYALCMYCGICVE
                     VCPFDALFWSPEYEYSEPRIADLLHDKDKLGEWMETVPEDAELEAGADKKGKK"
     gene            294..806
                     /locus_tag="PLV68_12140"
     CDS             294..806
                     /locus_tag="PLV68_12140"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HRE02042.1"
                     /translation="MLADALVAQNIAFGIIAAVMIYGAIRVVTATNVVHAALYLVGVL
                     GGAAAQYLLVYAEFVAVTQVMVYIGAVMVLFLFGVMLTRARIGKEDNLNNANAKIGIP
                     VAVLMFVAMSMVLIDGFKDDKLPGPKEATRVSVADVSDNIFGPYLLPFWALSFVLLAV
                     IGSIVLARKD"
     gene            810..1109
                     /gene="nuoK"
                     /locus_tag="PLV68_12145"
     CDS             810..1109
                     /gene="nuoK"
                     /locus_tag="PLV68_12145"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HRE02043.1"
                     /translation="MLINQFLLLGAVLFCIGVYGVIARKNAVMVLMSVELILNAVNLN
                     LLTFALRHGSADGHTFALYVIAIAAAEVGVGLGLVLLMFRKRRSIALDELSEMKG"
     assembly_gap    1137..1372
                     /estimated_length=236
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1373..>2210
                     /locus_tag="PLV68_12150"
     CDS             <1373..>2210
                     /locus_tag="PLV68_12150"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HRE02044.1"
                     /translation="ALRTVTAGGEEGEHHAAAYVEPVIRKVVWWQSNGIEIGLGQRID
                     GLAVMLLLLVAFISTLVQVFSLEYLRGDRRYTHFFAALTLFSAGMLSMVLAENMVQVI
                     LGWEIMGLCSFMLIGHWWEDYANSRAALKAFFTVRVGDVGLLVGTAIVFFGAQAWVNA
                     NPEAAHGLSGFSIDAINQWALGGDASKTMLLWGAVALFIACIGKSGQFPLHTWLPDAM
                     AGPTPVSSLLHSSTMVVAGVFLVARLYPVFHTGLHINVGDQQFNLIAVIGGITIVIAA
                     ALA"
     assembly_gap    2211..2220
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2221..>3154
                     /locus_tag="PLV68_12155"
     CDS             <2221..>3154
                     /locus_tag="PLV68_12155"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HRE02045.1"
                     /translation="IKKVLAYSTVSQLGYMMMGLGVGAWTPAVFHIFTHAFFKCCLFL
                     CAGSVSHSGSHHSFDMKKDMGGLAKKMPITTATWIVSTIALCGVPFFSGFFSKDEIID
                     FADHNGYTVFFVIGLVGAFMTTAYMTRATYLTFFGTPRGAAAGEHHDEHADAHDAHAA
                     HDAHDAHGHDAHDAHGHEAHGPHESPPLITVPLVILGVLAVISGYVNAAPFSIHKFTD
                     WVEPSLDGIHFPVLHHATFEWIKAVPSIVLVALGFVVSLGVCKALYGAKASPLKGLTQ
                     RVAPLRWGYNFLWNKYYLDALYEKVVVPVLVGPIS"
     assembly_gap    3155..3164
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3165..3425
                     /locus_tag="PLV68_12160"
     CDS             <3165..3425
                     /locus_tag="PLV68_12160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02046.1"
                     /translation="GAYWVTQNILDRAVNEAGIGARKTGTWVYRKIDQRVVDGAVNLS
                     GDVARGTGTALQPTSSGKINLYGALLFGAAAVAAIVLVIVNS"
     gene            3438..>3646
                     /locus_tag="PLV68_12165"
     CDS             3438..>3646
                     /locus_tag="PLV68_12165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HRE02047.1"
                     /translation="MDFISNDNWVLSVGTFLPLAGVIIMMLIPKSNELLHKQIALVTM
                     LATTAVGVFILFQFDYGNAGTLQFWA"
BASE COUNT          545 a         1125 c         1076 g          644 t
ORIGIN      
        1 tgcatctaca tcgagggcca caaggacctc gcgccgccgc gccgcgccgg tggtgcgccg
       61 cgcaaggtca acaagctcga ccgcttcgac atcgactacg cgctgtgcat gtactgcggc
      121 atctgcgtcg aggtctgccc cttcgatgcg ctgttctgga gccccgagta cgagtacagc
      181 gagccgcgca tcgccgacct cttgcacgac aaggacaagt tgggcgagtg gatggagacg
      241 gttcccgaag acgccgagct cgaagccggt gccgacaaga agggcaagaa gtagtgctcg
      301 ccgatgcgct cgtcgcccag aacatcgcct tcggcatcat cgccgcggtg atgatctacg
      361 gggccatccg ggtggtcacg gccaccaacg tcgtccacgc cgcgctgtac ctcgtgggcg
      421 tgctcggtgg cgccgcggcc cagtatctgc tggtgtacgc cgagttcgtt gccgtcaccc
      481 aggtgatggt gtacatcggc gccgtgatgg tgctgttcct cttcggcgtg atgctcaccc
      541 gggcccgcat cggcaaggag gacaacctca acaacgccaa cgccaagatc ggcatcccgg
      601 tggcggtgct gatgttcgtc gccatgtcga tggtgctgat cgacggcttc aaggacgaca
      661 agttgcccgg ccccaaggag gccacccggg tcagcgtggc cgacgtcagc gacaacatct
      721 tcgggccgta cctgctgccg ttctgggcgc tgtcgttcgt gttgctcgcc gtcatcggct
      781 cgatcgtgtt ggccaggaag gactgagccg tgctgatcaa ccagttcctc ctgctcggtg
      841 cggtgctgtt ctgcatcggt gtctacggcg tgatcgcccg caagaacgcg gtgatggtgc
      901 tgatgagcgt cgagctcatc ctcaacgcgg tcaacctcaa cctgctcacc ttcgccctgc
      961 gccacggcag cgccgacggt cacacgttcg cgctgtacgt cattgcgatc gcggccgccg
     1021 aggtcggcgt cggtctgggc ttggtgttgc tgatgttccg caagcggcgc tcgatcgcgc
     1081 tcgacgaact ctccgagatg aagggctgac ccgtgctcgc agcagaagca ggctggnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngggcgctg
     1381 cgcacggtca ctgccggtgg tgaagagggc gagcatcatg ccgcggccta cgtcgagccg
     1441 gtgatccgca aggtcgtctg gtggcagagc aacggcatcg agatcggtct cggtcagcgg
     1501 atcgacggcc tggccgtgat gctgctgttg ttggtggcgt tcatctccac gctggtgcag
     1561 gtgttctcgc tcgaatacct gcgtggcgac cggcgctaca cccacttctt cgccgcgctc
     1621 accttgttca gcgccggcat gttgtcgatg gtgctggccg agaacatggt ccaggtcatc
     1681 ctcggctggg agatcatggg cctctgctcg ttcatgctga tcgggcactg gtgggaggac
     1741 tacgccaaca gccgggccgc gctcaaggcg ttcttcacgg tgcgcgtcgg cgacgtcggc
     1801 ctgttggtcg gaacggcgat cgtgttcttc ggcgcccagg cctgggtcaa cgccaacccc
     1861 gaagccgcgc acggcctcag cgggttcagc atcgacgcga tcaaccagtg ggcgctgggc
     1921 ggcgacgcga gcaagacgat gctgctgtgg ggagccgtgg cgctgttcat cgcctgtatc
     1981 ggcaagagcg gtcagttccc gctgcacacc tggttgcccg acgccatggc cggccccacg
     2041 ccggtgtcgt cgctgctgca ctcgagcacc atggtcgtgg ccggcgtgtt cctggtcgcc
     2101 cgcttgtacc ccgtgttcca caccggcctg cacatcaacg tcggcgatca gcagttcaac
     2161 ctcatcgccg tgatcggtgg catcaccatc gtgatcgcgg ccgcgctggc nnnnnnnnnn
     2221 acatcaagaa ggtgttggcc tactccacgg tcagccagtt gggctacatg atgatgggcc
     2281 tcggcgtcgg tgcctggacg ccggccgtgt tccacatctt cacccacgcc ttcttcaagt
     2341 gctgcttgtt cctgtgcgcc ggctcggtca gccacagcgg cagccaccac agcttcgaca
     2401 tgaagaagga catgggcggg ctggccaaga agatgccgat caccaccgcg acgtggatcg
     2461 tgtcgaccat cgcgctctgc ggcgtgccgt tcttcagcgg cttcttcagc aaggacgaga
     2521 tcatcgactt cgccgaccac aacggctaca cggtgttctt cgtcatcggt ctggtcggtg
     2581 cgttcatgac caccgcgtac atgacccggg cgacctacct gaccttcttc ggcacgccgc
     2641 gaggggcggc cgcgggcgag caccatgacg aacacgcgga tgcccacgac gctcacgctg
     2701 cccacgacgc tcacgatgcg cacggacacg atgctcatga cgcgcacggg cacgaggccc
     2761 acggcccgca cgagtcgccg ccgctgatca ccgtgccgtt ggtgatcctc ggtgtgctcg
     2821 ccgtgatctc gggttacgtc aacgccgcgc cgttcagcat ccacaagttc accgactggg
     2881 tcgagcccag cctcgacggc attcacttcc ctgtgctgca ccacgccacg ttcgagtgga
     2941 tcaaggccgt gccgtcgatc gtgctggtcg cgctgggctt cgtcgtcagc ctgggcgtgt
     3001 gcaaggcgct gtacggcgcc aaggcctcgc cgctcaaggg tctgacccag cgggtcgcac
     3061 cgctgcgctg gggctacaac ttcctgtgga acaagtacta cctcgacgcc ctctacgaaa
     3121 aggtcgtcgt gccggtgctg gtcggaccga tctcnnnnnn nnnnggcgcc tactgggtca
     3181 cccagaacat cctcgaccgt gcggtcaacg aggccgggat cggcgctcgc aagaccggca
     3241 cctgggtcta ccgcaagatc gaccagcgtg tggtcgacgg cgcggtcaac ctctccggcg
     3301 acgtcgcacg cggtaccggc accgcgttgc agcccacttc gtcgggcaag atcaacctct
     3361 acggcgcgct gctcttcggc gccgccgcag tggccgccat cgttctcgtc atcgtcaaca
     3421 gctgaggaat cgcgctcatg gatttcatct ccaacgacaa ctgggtgctg agcgtcggca
     3481 cgttcttgcc gctcgccggc gtgatcatca tgatgctcat ccccaagagc aacgaactgc
     3541 tgcacaagca gatcgccttg gtcacgatgc tggcgaccac ggcggtgggt gtcttcatcc
     3601 tgttccagtt cgactacggc aacgccggca cgctccagtt ctgggc
//
LOCUS       DAORNF010000301         3636 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_19196_length_3636_cov_1.695616, whole genome shotgun sequence.
ACCESSION   DAORNF010000301 DAORNF010000000
VERSION     DAORNF010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3636
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19196_length_3636_cov_1.695616"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="PLV68_12170"
     CDS             <1..350
                     /locus_tag="PLV68_12170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02048.1"
                     /translation="PITPQRVIDPTTATAEVHLATGVFVDEWRARPDAIEVATFDPRS
                     PAERDEFDRLHAELIGELTRLGLTDLIETVDGNLFGVQVDSRIPEHLQATVRHMLELG
                     VESAVFIAPPPEF"
     gene            complement(406..1464)
                     /locus_tag="PLV68_12175"
     CDS             complement(406..1464)
                     /locus_tag="PLV68_12175"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRE02049.1"
                     /translation="MTTALVTGGSGYFGSLLTRALLDAGQQVRVFDINAPDTGDATTT
                     GAEVAIGDIRDPAAVRAAVDGIDVVYHNVAQVPLANDAHLLRSVNVDGTRILLDACAD
                     AKVAKVVHTSSSAVFGVPESTPVLPSTVPKPAEAYGHAKLAAEWACLDAAAKGLDVSI
                     VRPRTILGHGRLGIFGILFDWIADGVDVFVLGNGANRYQFVHADDLADVCIRAGAASG
                     PLIVNAGTDRFGTMRETLESVCAHAGTGATVRSLPAGPASLAMRAAAGARIAPFAPYH
                     WIMYSRSMWFDIAHATEALGWSPRWSNEEMFAQSYDWFVAHRAGLDASDQSASHHRRP
                     AKQGALAMMKWATRLLPR"
     gene            complement(1461..>2026)
                     /locus_tag="PLV68_12180"
     CDS             complement(1461..>2026)
                     /locus_tag="PLV68_12180"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRE02050.1"
                     /translation="AELAVRQAAGDGLDAVIVRPPWFYGPLQPPRQTTFFRMVRTGRF
                     PIFGRGDQRRSMVYLDNLVQGVVRAELTDAARGNGYWIADARPYTVTEIVATVGRALR
                     DEGYEVKGGGMKMPVLVGRIAERADRILQGAGRYQQQLHVLGEMAHSIACDISAAQAE
                     LGYDPQVELYEGMRRSIQWCRAEGLDL"
     assembly_gap    2027..2052
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2053..>2324)
                     /locus_tag="PLV68_12185"
     CDS             complement(<2053..>2324)
                     /locus_tag="PLV68_12185"
                     /inference="COORDINATES: protein motif:HMM:NF013255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRE02051.1"
                     /translation="EVVVGDVADASSLTPLFADAPADTDVIHTAGVIHPASVAEFFRT
                     NATGTANVVEASVGAAVRRLVHVSSNSPFGTNPSTGDVFRTNDAYNP"
     assembly_gap    2325..2469
                     /estimated_length=145
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2535..>3121)
                     /locus_tag="PLV68_12190"
     CDS             complement(2535..>3121)
                     /locus_tag="PLV68_12190"
                     /inference="COORDINATES: protein motif:HMM:NF017050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HRE02052.1"
                     /translation="APEISFGGSASNPGLLAMALNDNWASFGAAMADWQRISVYYAAI
                     TSQGTGRVIAVPGLRDPLVTYRLTARDKALLGSGLSRLALCMLAAGADEVYPSFRGAP
                     LVRNEADVAQLRDAFSVNRATVMSVHLCSTVPLGEHPRCAADSYGRVRGMRNLWVNDA
                     SLLPEAPGVNPQGSVMAFAIRNARHFIATERPHG"
     assembly_gap    3122..3164
                     /estimated_length=43
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3165..3617)
                     /locus_tag="PLV68_12195"
     CDS             complement(<3165..3617)
                     /locus_tag="PLV68_12195"
                     /inference="COORDINATES: protein motif:HMM:NF012935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HRE02053.1"
                     /translation="MHAIGTEIESALHVVTVPGEVSPASAVLRDGASALGWANDESPR
                     WMTYPPGGDAGSGRRQSMTKTYLPRAQRAGARVVTNCRVRRLDLDRSVARRALVELAD
                     GSPGTITFRHVFVCAGAIHTPALLQRSGLRHRIGTRLAVHPTVKLSARF"
BASE COUNT          514 a         1131 c         1266 g          511 t
ORIGIN      
        1 tgccgatcac cccgcagcga gtgatcgacc cgaccacggc gaccgccgag gtgcatctcg
       61 cgaccggtgt gttcgtcgac gaatggcgcg cccgtcccga cgcgatcgaa gtcgccacct
      121 tcgatccgcg cagcccggcc gaacgcgacg agttcgaccg tctccatgcc gagctgatcg
      181 gcgaactgac ccgcctgggc ctgaccgatc tgatcgagac ggtcgacggc aatctgttcg
      241 gggtgcaggt cgacagccgc atccccgagc atctccaggc aaccgtgcga cacatgctcg
      301 aactcggcgt cgagtcggcc gtgttcatcg cgccaccacc agagttctga acggtcgccg
      361 gatggtgagc ggctccggag tgccgagccg ctgtcgaaat gccggtcagc gggggaggag
      421 gcgggtcgcc cacttcatca ttgccagtgc gccctgcttg gccggacggc gatggtgcga
      481 cgccgactgg tcgctggcgt cgaggccggc ccgatgggcg acgaaccagt cgtagctctg
      541 ggcgaacatt tcctcgttcg accaccgcgg tgaccatccg agcgcctcgg tcgcgtgggc
      601 gatgtcgaac cacatcgaac ggctgtacat gatccagtga tacggcgcga acggagcgat
      661 gcgcgcgcca gccgcggcgc gcatcgccag tgatgccggg ccggcaggca gcgagcgcac
      721 ggttgcgccg gtaccggcgt gggcgcacac gctctccaac gtctcgcgca tggtgccgaa
      781 gcggtcggtg ccggcgttga cgatcagcgg accgctcgcc gccccggcgc ggatgcacac
      841 gtcggcgagg tcgtcggcgt ggacgaactg gtagcggttg gctccgttgc cgagcacgaa
      901 cacgtcgacc ccgtcggcga tccagtcgaa caggatgccg aagatgccga gccggccgtg
      961 accgagaatg gtccggggcc gcacgatgct gacgtcgagc cccttggcag cggcatcgag
     1021 gcacgcccat tcggcggcga gcttcgcatg gccgtacgct tcggcgggct tgggcacggt
     1081 ggagggcagc accggggtgg actccggtac cccgaacacc gcgctggaac tggtgtggac
     1141 tactttggcg accttcgcgt cggcgcacgc gtcgagcagg atgcgcgtgc cgtcgacgtt
     1201 gaccgaccgc agcaggtgcg catcgttggc cagcggtacc tgggccacgt tgtggtacac
     1261 cacatcgatg ccgtcgaccg cggcacgcac ggcagccgga tcacggatgt cgccgatcgc
     1321 gacctcggcc cccgtcgtgg tggcgtcacc ggtatcgggc gcgttgatgt cgaagacgcg
     1381 cacctgttgg cccgcgtcga gcagggcgcg agtgagcaga gagccgaagt agccgctgcc
     1441 tccggtgacc agggcggtgg tcacagatcg agtccctcgg cgcggcacca ctggatactg
     1501 cgccgcatcc cttcgtacaa ctcgacctgc gggtcgtagc cgagctcggc ctgcgctgcg
     1561 ctgatgtcgc aggcgatcga gtgggccatc tcgcccagca cgtggagctg ttgctgatag
     1621 cggccggcgc cctggaggat gcgatcggcc cgctcggcga tgcgcccgac cagcaccggc
     1681 atcttcatgc cgccgccctt gacctcgtag ccctcgtcgc gcagtgcccg cccgacggtg
     1741 gcgacgatct cggtcaccgt gtacggcctg gcgtcggcga tccagtaacc attgccgcgg
     1801 gcggcgtcgg tcagttcggc gcgcaccaca ccctgcacca ggttgtcgag atagaccatc
     1861 gagcggcgct ggtcgccgcg cccgaagatc ggaaagcgcc cggtgcgcac catgcggaag
     1921 aacgtcgtct ggcgcggtgg ctggaggggg ccgtagaacc acggcgggcg gacgatgacg
     1981 gcgtcgagtc cgtcgccggc agcctggcgc accgccagtt cggcctnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nngggttgta ggcgtcgttg gtgcggaaca cgtcgccggt ggacgggttg
     2101 gtgccgaagg gtgagttgga cgacacgtgg accaaccgtc gcaccgcggc acccacgctg
     2161 gcctcgacca cgttggccgt gccggtggcg ttggtgcgga agaactcggc gaccgatgcc
     2221 gggtggatca cacccgcggt gtggatcaca tcggtgtcgg cgggggcgtc cgcgaacagg
     2281 ggcgtgagcg aggatgcgtc ggccacgtcg ccgaccacca cctcnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnng agcggagcgg gtggacgggg tggagcgggt ggacggggtg gagcgggtgg
     2521 acggggtggt cggttcagcc atggggacgc tccgttgcga tgaagtggcg ggcattgcgg
     2581 atggcgaagg ccatcaccga tccttgcggg ttgacgccgg gggcttcggg cagcagcgac
     2641 gcgtcgttga cccacaggtt gcgcatgccg cgcacccgcc cgtagctgtc ggccgcgcag
     2701 cgcggatgct cgccgagggg cacggtcgaa cacaggtgga cactcatcac ggtggcgcgg
     2761 ttgaccgaga acgcatcgcg cagttgggcg acgtcggctt cgttgcgcac cagcggcgca
     2821 ccgcggaagc tgggatacac ctcgtcggcc ccggcggcca gcatgcacag cgccagacgg
     2881 ctgaggccgc tgccgagcag ggccttgtcg cgggcggtga ggcggtaggt gaccagcggg
     2941 tcgcgcaacc ccggcaccgc gatcacccgg ccggtgccct ggctggtgat cgccgcgtag
     3001 tagacgctga tccgctgcca gtcggccatc gccgcgccga agctggccca gttgtcgttg
     3061 agcgccatcg ccagcaggcc ggggttgctg gccgaaccgc cgaagctgat ctcgggggcg
     3121 annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngaagcg ggctgagagc
     3181 ttgacggtgg ggtgcactgc cagccgggtg ccgatccggt ggcgcaatcc gctgcgctgc
     3241 aacagcgccg gggtgtggat cgcccccgcg cagacgaaca cgtggcggaa cgtgatcgtg
     3301 cccggtgaac cgtcggccag ttcgaccaat gcccgccggg cgacgctgcg gtcgaggtcg
     3361 aggcgccgca cccggcagtt ggtgacgacg cgcgcccccg cccgctgggc gcggggcaga
     3421 tacgtcttgg tcatgctctg gcgtcgcccg ctgcccgcgt cgccgcccgg cgggtaggtc
     3481 atccagcgcg ggctctcgtc gttggcccag ccgagcgcgc tggcaccgtc gcgcagcacg
     3541 gcactggcgg gggagacctc gccgggcacc gtgacgacgt gcaacgcaga ttcgatctcg
     3601 gtgccgatcg catgcatgtg gtcggggtcg aggtcg
//
LOCUS       DAORNF010000302         3633 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_19224_length_3633_cov_1.597261, whole genome shotgun sequence.
ACCESSION   DAORNF010000302 DAORNF010000000
VERSION     DAORNF010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3633
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19224_length_3633_cov_1.597261"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /locus_tag="PLV68_12200"
     CDS             complement(<1..428)
                     /locus_tag="PLV68_12200"
                     /inference="COORDINATES: protein motif:HMM:NF013306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biliverdin-producing heme oxygenase"
                     /protein_id="HRE02054.1"
                     /translation="MTTTEEIDVTHPFSETIKNASWEVHERAEQLPFVQRLMSGKASF
                     DEYADMVAQHYFAYRALERFRDEIATDPIAGPFAAPELLRVPALEDDLEALYGPDWRD
                     RIAPSAATLAFVARLEEVGSTWPGGYIAHHYVRYLGDLSGG"
     gene            637..>1422
                     /locus_tag="PLV68_12205"
     CDS             637..>1422
                     /locus_tag="PLV68_12205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02055.1"
                     /translation="MIARVAGRVMAVCLLALAPLVVMPIATPATASAQPGGTAPVCTT
                     PTPVGPPVLTASKTTGLDPDGDTVVLSGSNYNECHGIYVAMCVMPPTGQLPSPCGGGE
                     DRDGDTGASKWIATYPPSYGVGLAKPYGPNGTFTETLKVSAVIAADADCRYVQCVLVT
                     RNDHTRTSDRSQDVMIPVFFDETSPTTTTTTTAPPATTTTAPRPVATTTTVPSTTTVP
                     STTTAPSTTTTVAQSTTTTAVEATSTVAESTTTTAPLPSSTTSS"
     assembly_gap    1423..1443
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1444..1652
                     /locus_tag="PLV68_12210"
     CDS             <1444..1652
                     /locus_tag="PLV68_12210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02056.1"
                     /translation="TTSSTAPIETLGTVAPDDDGDGGSGLALAIGAGLVAVGLAGGIA
                     VWRHRRRPAAPGLIPDSADDPPLD"
     assembly_gap    1775..1784
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1785..2783
                     /locus_tag="PLV68_12215"
     CDS             <1785..2783
                     /locus_tag="PLV68_12215"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRE02057.1"
                     /translation="SVATGGAPVPPSNTDPAGSDTSTSLLPLYVPPSTTAPPHVDTEP
                     LVPISTDARPALPATITGDDGRDVTVTDVSRILPLWGNLSEVVFTLGLGANVVGRDVA
                     ATFNEVEDLPVVTHGHDVSAESVLSLRPTVVLAQTDTGPPEAIEHIRNAGVPVVVVKA
                     PESFADIDERITLVAAALGVGDLGVQLIERTHTEIAEVQATIPAGDPPRIAFLYLRGT
                     AGVYLIAGPGSGADSMIAAAGGVDAGTAMGLERPFTPLTSEAFVVAAPDVILMTTTGL
                     ASVGGVDGLVKIPGVAQTPAGIDRRVITVEDGLLYSFGSRTPEALREMIEQIYGAP"
     gene            complement(2810..>3019)
                     /locus_tag="PLV68_12220"
     CDS             complement(2810..>3019)
                     /locus_tag="PLV68_12220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02058.1"
                     /translation="DHQRHRQHWQHQPARPAHRHDPSFDGNLVRVARTGITSAGVTGA
                     VVTGAGARSVRVSRADSALDRSFTG"
     assembly_gap    3020..3296
                     /estimated_length=277
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3297..>3633
                     /locus_tag="PLV68_12225"
     CDS             <3297..>3633
                     /locus_tag="PLV68_12225"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HRE02059.1"
                     /translation="IVVGFTLLDTWSVAVTAFIGGLITVVGVYAASRHDGRTEVVTLI
                     LTGVAVNAMTGAIIGLLTYFSTDAELRSITFWQLGSMAQATWPKVAAVAPLALIGIVA
                     ALTRARSLDL"
BASE COUNT          500 a         1180 c         1105 g          540 t
ORIGIN      
        1 ccgcccgaca ggtcgccgag gtagcggacg tagtggtggg cgatgtagcc accgggccag
       61 gtgctgccga cctcttccag ccgggcgacg aacgcgagcg tcgccgcgct cggagcgatg
      121 cgatcgcgcc agtcaggtcc gtacagcgct tccagatcgt cctccagcgc gggcacccgc
      181 aacagctccg gtgccgcgaa tggaccagcg atcgggtctg tcgcgatctc gtcacggaac
      241 cgttccaagg cgcggtaggc gaagtagtgc tgggcgacca tgtcggcgta ctcgtcgaag
      301 ctggccttgc cgctcatcag ccgctgcacg aacggcaact gctcggcccg ctcgtgtacc
      361 tcccacgacg cgttcttgat cgtctcggag aacgggtggg tgacatcgat ctcttcggtg
      421 gtggtcatcg ccgaaatccc tcctggcagc cccgctgcgg ctcgcgtgcg caggggatcg
      481 gtccagtgtg cagcaccgat gaacggtctt caacacgttc acgacgattt gtcgtgaaaa
      541 tgtggaccac gaccgatggt cgtgtagcct gctccagcga tggtagggca tcctaacgac
      601 tgctgcgggg gttctcggcg cggcatgatc gtcacggtga ttgcgcgcgt cgctggccgg
      661 gtgatggccg tgtgtctgtt ggcgttggcg ccgctggtgg tgatgccgat cgcaacgccc
      721 gccacggcgt ccgcgcaacc gggcggtacc gcgccggtct gcaccacgcc cacaccggtc
      781 ggcccgccgg tgttgaccgc gtcaaagacc actgggctcg accccgacgg cgacacggtg
      841 gtgctcagcg gatcgaacta caacgaatgc cacggcatct acgtggcgat gtgtgtcatg
      901 cctccgactg gtcaactgcc cagcccctgc ggtggtggcg aggatcgcga cggcgacacc
      961 ggggcgtcga agtggatcgc cacctacccg ccgagctatg gcgtcggctt ggccaagccg
     1021 tacgggccca acggcacctt caccgagacg ctgaaggtgt cggccgtcat cgccgccgat
     1081 gccgattgcc ggtacgtgca gtgcgtactc gtcacccgca acgaccacac ccgcaccagc
     1141 gaccgcagcc aagacgtgat gatcccggtc ttcttcgacg agaccagtcc cacgaccacg
     1201 accactacca cggcacctcc ggcgaccacg accacggcgc cacggccggt tgccacaacc
     1261 acgaccgttc catcgacgac gactgttcca tcgacgacga ccgctccatc gacgacgacc
     1321 acggttgcgc agtccaccac gaccaccgcg gtcgaggcca cgagcacggt tgcggagtcg
     1381 accaccacga cggcgccctt gccgtcgtcc accacctcca gcnnnnnnnn nnnnnnnnnn
     1441 nnnccaccac gtcgagcacc gcaccgatcg agacgttggg gacggttgcg cccgacgacg
     1501 atggtgacgg cggctccggt ttggcgctgg cgatcggcgc gggtctggtc gcagtcggcc
     1561 tggccggtgg catcgcggtc tggcgccacc gccgccgccc ggccgctccc ggcctgatcc
     1621 ccgacagcgc tgatgacccg ccgctcgatt gatcctgact gctcggctga cgtcctgggc
     1681 cgttctggcc gcgttggccg gctgctggtg gcgaccctgc ttgcggtcgc gttcgctgcg
     1741 ggttgttcta ccagcagcaa cgatgccgac ggttnnnnnn nnnnagcgtc gcgaccggcg
     1801 gcgcacccgt gccaccgagc aacaccgacc ctgctggcag cgacacgtcg acctcgctgt
     1861 tgccgttgta cgtgccgccg tcgaccaccg cgccaccgca cgtcgacacc gaaccgctgg
     1921 tcccgatcag caccgacgcc cgcccggcgt tgcccgccac catcaccggt gacgacggcc
     1981 gcgacgtgac cgtcaccgac gtcagccgca tcctgccgct gtggggcaac ctgtccgagg
     2041 tggtgttcac cttggggctg ggcgccaacg tcgtcggccg cgacgtcgcc gcgacgttca
     2101 acgaggtcga agatctaccc gtcgtcaccc acggccacga cgtgtcggcc gagtcggtgc
     2161 tgtcactgcg ccctacggtg gtcttggcgc agaccgacac cggtccgccc gaagcgatcg
     2221 agcacatccg caacgctggt gtgccggtgg tcgtggtgaa ggcgcccgag agcttcgccg
     2281 acatcgacga gcggatcacg ctcgtcgccg ccgcgctcgg ggtcggcgat ctcggcgtgc
     2341 aactgatcga gcggacccac acggagatcg ccgaggtgca ggccaccatc cccgccggtg
     2401 atccgccgcg gatcgccttt ctgtacctgc gcggcacggc cggggtgtac ctcatcgccg
     2461 ggccaggctc cggggccgac tcgatgattg cggctgcagg tggggtcgac gccggcactg
     2521 cgatggggct cgagcgaccg ttcacgccgt tgaccagcga agcgttcgtg gtggccgctc
     2581 ccgacgtcat cctcatgacc accaccgggc tggcctcggt cggcggcgtc gacgggctgg
     2641 tcaagattcc tggcgtggct cagaccccag ccggcatcga ccggcgtgtg atcacggtgg
     2701 aagacggtct gctgtacagc ttcgggtcgc gcacacccga ggcgctgcgg gagatgatcg
     2761 agcagatcta cggcgcacca tgaccgcgac cggcgaccga gctgtccagt catcccgtga
     2821 acgaccggtc gagcgccgag tcggcccggc tcacgcggac gctgcgagcc ccggcgccgg
     2881 tgaccacggc gccggtgacc ccggcgctgg tgatcccggt gcgagcgacg cggacaaggt
     2941 tgccatcgaa cgacggatcg tggcgatgcg ccgggcgcgc cggttggtgt tgccagtgct
     3001 gacggtggcg ttggtggtcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncgat
     3301 cgtcgtcggc ttcacgctgc tcgacacgtg gagcgttgcc gtcaccgcgt tcatcggtgg
     3361 cctgatcacc gtcgtgggcg tctatgccgc gtcgcgccac gacgggcgca ccgaggtggt
     3421 caccctgatc ctcaccggtg tcgcggtcaa cgccatgacc ggcgcaatca tcggcctgct
     3481 gacgtacttc tccaccgatg ccgagctgcg ctcgatcacg ttctggcagc tgggcagcat
     3541 ggcccaggcc acctggccca aggtggccgc ggtcgccccg ttggcgttga tcggcatcgt
     3601 cgccgcgttg acgcgcgccc gctcgctcga tct
//
LOCUS       DAORNF010000303         3632 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_19233_length_3632_cov_1.769919, whole genome shotgun sequence.
ACCESSION   DAORNF010000303 DAORNF010000000
VERSION     DAORNF010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3632
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19233_length_3632_cov_1.769919"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    338..537
                     /estimated_length=200
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<538..1987)
                     /locus_tag="PLV68_12230"
     CDS             complement(<538..1987)
                     /locus_tag="PLV68_12230"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HRE02060.1"
                     /translation="MDADPSSTDSWLEVAAISEMGQAVAEFDWSSTPLGGFDTWPDGL
                     KLAVSVCLSSRFPILVTWGPDLIKIYNDGYRQILGAEKHPLALGSPAKEVWPEVWDYI
                     GPLFDSVMATGIPTWAEDEELVLHRSGFAEECYFTFSYSPLFDNGKIHGVLDLVAETT
                     GHVLARRRLECLSELQRALSAAEHVTDVCARAVAATTRFPRDLRACDIYLSVEGDLVL
                     VATNRRGDVAPVDHDELLQIAEQRVPTARGGDPDGATPSMEFIVPLGSAFGGVTGLLV
                     LSLNPRRPFDSAYRHFGTLLADAISASLDGAFRRSSELGEYRRISDTLQASMLKPASD
                     LPTVAARYLPAVGNLAVGGDWYDVIDLGNNQRGLIVGDCVGHGLDAATVMAQLRSAAR
                     ALMLEGQAPADVLDQLDLFACSIDGAECATVVCAVFDRARRTFTYSRAGHVPPLLLQR
                     NTSVWLDQAAGPPLTITGRSPRSNAIVAVSDDD"
     assembly_gap    2248..2274
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2275..2603
                     /locus_tag="PLV68_12235"
     CDS             <2275..2603
                     /locus_tag="PLV68_12235"
                     /inference="COORDINATES: protein motif:HMM:NF014517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HRE02061.1"
                     /translation="LLHVSAASVLTLEAAGEMAAGAAELFGADVTVATTGVAGGEAID
                     GTAPGTVYIATSVDGVVSSCDYRFQGTPESVCDQARHQALVQLANALAAGDGRVRSSF
                     DDEREG"
     gene            2639..>2839
                     /locus_tag="PLV68_12240"
     CDS             2639..>2839
                     /locus_tag="PLV68_12240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02062.1"
                     /translation="MPTKSANIKNEKQYEALKDKGMSKERAAKIANSPDASKHGGEKS
                     HSGSGKSNQGGTTAQHKAAGRKG"
     assembly_gap    2840..2850
                     /estimated_length=11
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2851..3031
                     /locus_tag="PLV68_12245"
     CDS             <2851..3031
                     /locus_tag="PLV68_12245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02063.1"
                     /translation="DRSQEVLTARHLASGDAPSTSLSSEQPPVYAQTEPCWNHEQHLG
                     DIRGHPRCQSAADST"
     gene            complement(3007..>3632)
                     /locus_tag="PLV68_12250"
     CDS             complement(3007..>3632)
                     /locus_tag="PLV68_12250"
                     /inference="COORDINATES: protein motif:HMM:NF018648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HRE02064.1"
                     /translation="VGEASHRSARFWLPRETAARELEAEQLVDVLENDDDIRADEVRA
                     TATLIRGSVSACDRLIDALHPWTSYLIVPIFALANAGIALSTSSITSPSRVLGGVAIA
                     LVAGKFVGVTLFSWITVRLGIGRLPDEARWGHVLGVAALAGIGFTVSLFITGLAFDDA
                     TLQADAKTGILIASVVAAGVGAAVLAAVARNGTECQANQGQVESAAD"
BASE COUNT          621 a         1056 c         1162 g          555 t
ORIGIN      
        1 cccgcgcatc tccgggtacg tcacgcgcag gtcggcggcg agccgttcga tcaccttggt
       61 gccgtaggtc tcggcgtttt ggcgataaga acagcgctcc cggcaagatt cgaacttgcg
      121 acgcacggtt taggaatgag cgcgagtggg tctcagcgag tcaccttgaa tcgcatctga
      181 cctgctcagc ttctgtgttc tcctcgtctg gtctcgttga acatcatgca gcagcgcgca
      241 gtagatggac aaatggatgg acaactttgg gcccgtcaat cgtcacggcg tgaccgtccg
      301 ccgaggccga tcaactcaat ggttggtcag gacttggnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncgt
      541 cgtcatcgct gacggcaacg atggcgttcg aacgtgggga tcttccggtg atggtgagcg
      601 gcggtccggc ggcctgatcg agccacaccg aggtgtttcg ctgcaggagc agtggtggga
      661 cgtgcccagc acgtgagtag gtgaacgtac gcctggcgcg gtcgaacacg gcgcagacga
      721 cggtggcgca ctcagcaccg tcgatcgagc aagcgaagag gtcgagttgg tcgagcacgt
      781 cggcgggggc ttgaccttcg agcatcaggg cgcgggcggc gctgcggagt tgcgccatca
      841 cggttgccgc gtcgaggccg tgaccgacgc agtcgccgac gatgagtcca cgttggttgt
      901 tgccgaggtc gatgacgtcg taccagtcgc cgccgacggc gaggttgccg acggccggca
      961 ggtagcgggc ggcgacggtc gggaggtcgc tggccggctt cagcatcgac gcttgcaagg
     1021 tgtcgctgat cctgcggtac tcgccgagct cactcgatcg acggaacgcc ccgtcgagcg
     1081 aggccgagat cgcgtccgcg agcagggtcc cgaagtgtcg gtaggcgctg tcaaacggtc
     1141 gtcggggatt gagggagagc acgagcagac ctgttacgcc accgaacgcg ctgccgagcg
     1201 ggacgatgaa ctccatcgac ggtgtcgctc cgtcggggtc gccgccgcgt gccgtgggaa
     1261 ctcgttgctc ggcgatctgc agcagttcgt cgtggtcgac gggagcgacg tcgccgcgtc
     1321 gattggtcgc gacgagcacg aggtcgccct cgacgctgag gtagatgtcg caggcacgta
     1381 ggtcgcgggg gaatctggtg gtcgccgcta cggcgcgagc gcacacgtcg gtgacgtgtt
     1441 cggctgcgct gagcgctctc tggagctccg agaggcattc cagccgccgg cgtgcgagga
     1501 catgaccggt ggtctcagcg accaggtcca acacaccgtg gatcttcccg ttatcgaaga
     1561 gggggctgta ggagaacgtg aagtagcact cctcggcgaa gccgctccgg tggaggacga
     1621 gttcctcgtc ctccgcccac gtcgggatgc ccgtcgccat caccgagtcg aacagcggac
     1681 cgatgtagtc ccacacctcc ggccagacct ccttggcagg cgagccgagc gccaaggggt
     1741 gcttctcagc gcccaggatc tggcggtagc cgtcgttgta gatcttgatc agatctggtc
     1801 cccacgtgac gaggatcgga aagcgcgacg acagacacac cgacacggcg agtttgagac
     1861 cgtccggcca cgtgtcgaac ccgccgagtg gtgtggacga ccagtcgaac tcggcgactg
     1921 cctggcccat ctcagagatc gccgcgacct ccaaccaact gtcagttgat gaagggtcgg
     1981 cgtccatcaa cgatgatcgc aattcgggcc gtagcgccga gctttctcgt gggacacgga
     2041 gccgcacatc ccgccgacca ggcaggacag ccaggtcagt gtgtcgatct aacgttctga
     2101 tcgtggacga cgaggcgttg gcggcatgcg tggcgagtct cctcgctggg cgatcgatcg
     2161 cgacagcgga gtcctgtacc gccggccgca tcgcagaggt gctcgcctgc gtcgagggag
     2221 cgagcgactt ccttcgcggc ggtctggnnn nnnnnnnnnn nnnnnnnnnn nnnnagttgc
     2281 tgcacgtgag cgcggcgtcg gtgctgaccc tcgaggccgc gggcgaaatg gccgccgggg
     2341 cagctgagct gttcggcgcg gacgtgacgg tggcgacaac tggtgtcgct ggcggtgagg
     2401 ccatcgacgg gacggcaccc ggcaccgtgt acatcgcgac ctccgtggat ggtgtcgtat
     2461 ccagctgcga ttaccgcttc cagggcacac ccgaatccgt ctgcgatcaa gcacggcatc
     2521 aggcgctcgt gcaactcgcc aacgcgctgg ccgcaggcga tggtcgagtt cggagcagtt
     2581 ttgacgacga gcgcgaaggg taacgattcg ccatccgacg caccgaagga ggcatgcgat
     2641 gcccaccaaa tctgccaaca tcaaaaacga gaaacagtac gaggccctca aggacaaagg
     2701 aatgtcgaag gagcgggcgg ccaagatcgc caactcgccc gacgcctcca agcatggcgg
     2761 cgagaagtcg cactcgggtt cgggcaagag caaccagggc ggcaccaccg cccaacacaa
     2821 agccgccggc cgcaaaggtn nnnnnnnnnn cgaccgcagc caagaagtcc tgaccgcacg
     2881 acacctcgca tcgggcgacg caccgtcgac atccttgagc agcgaacagc cgcccgtcta
     2941 tgcgcagacc gaaccgtgtt ggaaccacga gcaacatctc ggcgacatcc gcggacatcc
     3001 tcgctgtcag tcggcggcgg actccacttg accttggttg gcttgacatt cggttccgtt
     3061 ccgagcgacg gcagcgagca cggccgcacc gacaccggcg gcgaccactg acgcgatgag
     3121 gatgccggtc ttggcgtcgg cttggagggt ggcgtcgtcg aatgcgagtc cggtgatgaa
     3181 cagtgagacg gtgaagccga tcccggcgag cgcggcgact ccgagcacat gcccccaccg
     3241 ggcctcgtcg ggtagccggc cgatgccgag ccgcaccgtg atccagctga acagcgtgac
     3301 gccgacgaac ttgccggcca cgagcgcaat ggcgacaccg ccgagtaccc gtgacggaga
     3361 cgtgatcgaa ctcgtggaga gcgcaatgcc agcgttggcc agcgcgaaga tcggcacgat
     3421 caggtagctc gtccaggggt gcagtgcgtc gatcagccgg tcgcaggccg agaccgatcc
     3481 tcggatcagg gttgcggtgg cccgtacctc gtcggcgcgg atatcgtcgt cgttctccag
     3541 cacgtcgacg agttgctcgg cctccagctc gcgtgccgcc gtctctcgag gaagccagaa
     3601 ccgcgcggag cgatggctcg cttctcccac gc
//
LOCUS       DAORNF010000304         3623 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_19292_length_3623_cov_1.829877, whole genome shotgun sequence.
ACCESSION   DAORNF010000304 DAORNF010000000
VERSION     DAORNF010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3623
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19292_length_3623_cov_1.829877"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>691)
                     /locus_tag="PLV68_12255"
     CDS             complement(<1..>691)
                     /locus_tag="PLV68_12255"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRE02065.1"
                     /translation="IFLHGWGLANHTYRSVVSRVAAQGCRVYAPSLPGFGGTGELPKD
                     EFSIAGFARWVAAFVKAMHIDEPAIAIGHSFGGGVSIRFAHDNPELVRSLVLVNSIGG
                     SSWRRGEVIRSISERPLWDWGLHFPSDVWPIRQATRVLPVILEDFVPNVLRNPRAVVK
                     VSNLARRADLRDELEHLRERGLPITILWGTRDGIIPRESFEAMCVAAGVEGTVVEGSH
                     SWLLADPDRFG"
     assembly_gap    692..701
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(824..1090)
                     /locus_tag="PLV68_12260"
     CDS             complement(824..1090)
                     /locus_tag="PLV68_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HRE02066.1"
                     /translation="MSRSVDSDDPDWQLDAACRGQDPAIFYPPPFFERKELRRAREAV
                     AKSICAQCPVSHACLQQALRTEEPHGVWGGLNEFERRERISRSA"
     gene            complement(1193..1894)
                     /locus_tag="PLV68_12265"
     CDS             complement(1193..1894)
                     /locus_tag="PLV68_12265"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HRE02067.1"
                     /translation="MAYLLFAIGLTLLIFEFFTAGVGIAGVVGAVCTLLACYGLAALP
                     VRGWAVAVLLFSMFAFAIDVQVGVPRLWTGIGMAAFVIGSWFLFADVLGTTVRPSWIT
                     LLTGIIGIALSFVVGMPSMVRTRFATPTVGREWMIGEMGKAVGAIDPEGMAEVAGGRW
                     RARVNRATPLQHGDVLRVVAIDGVTLEVEPEEGGAKDYREMRNRKQNAHGATPDAETD
                     ATGIDADTAEAEAGH"
     gene            complement(1864..2535)
                     /locus_tag="PLV68_12270"
     CDS             complement(1864..2535)
                     /locus_tag="PLV68_12270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02068.1"
                     /translation="MAAPVDPSDDSVDHLAPVDVVQVSGLIDEILIDEIEVAIRDAVA
                     NGSQALILQVNSPGAVVGRDRMTDLVETLRDAPIPIGVWVGPSGAKLTGLGMQMMAAA
                     DATAMAPGTHIGKMGAPLPVANVSFDFGAATERLRSESMGFQEARRAGALKLSITDEG
                     VPAVRNMALALDGLTARGPSRSTPTAARRSTPPCCASPSSVWSTSCCTPSPARRWPIC
                     CSPSA"
     gene            2567..3601
                     /locus_tag="PLV68_12275"
     CDS             2567..3601
                     /locus_tag="PLV68_12275"
                     /inference="COORDINATES: protein motif:HMM:NF014433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HRE02069.1"
                     /translation="MAGSALAADSPIPDRRVATAALAKRVIASLRKEMASGGRVETQQ
                     VFGAARLVIVAGKGGVGKTTVTAVIAHAAAAAGLRVAAIDLEGKPALANMLADSPVEV
                     TTITASDALGEYLDTHGLGRVAKRLANSGVIEVVATAAPGIDDIVVLGKLKQLERSGE
                     YDLVVVDGPAAGHAITFLLSPAGLLETVRSGPIRVQAQDVLEMFADPQRCQVVLVGLP
                     EATPINELIETAYMIEERVGVQLGPVVMNGVDPGPEVVLSGTGDLSADDPLIAAAEFR
                     NHRLAWQRGELERLAGELALDRYVLPQLPVAGISAADVATLAAALVAQLGDRAWDAPR
                     PQTPVAPVTR"
BASE COUNT          589 a         1252 c         1224 g          548 t
ORIGIN      
        1 tcgccgaagc ggtcggggtc ggccagcaac cacgaatgcg acccctccac caccgtgcct
       61 tcgacacccg cggcgacgca catcgcctcg aacgactcgc gcgggatgat gccatcgcgc
      121 gtgccccaca ggatcgtgat cggcagaccc cgctcgcgga gatgttccaa ctcgtcgcgc
      181 aggtcggcgc ggcgggccag gttcgacacc ttcacgacgg cgcgcgggtt gcgcaacacg
      241 ttgggcacga agtcttcgag gatcacgggg agcacgcggg tcgcctggcg gatcggccag
      301 acgtcactgg gaaagtgcag gccccagtcc cacagcggcc gctcggagat cgagcggatc
      361 acctcacccc ggcgccatga cgatccgccg atcgagttga ccagcaccaa cgagcgcacc
      421 agttccgggt tgtcgtgggc gaaacggatc gacaccccgc cgccgaagct gtgcccgatc
      481 gcgatcgccg gttcgtcgat gtgcatcgcc ttgacgaacg ccgccaccca ccgcgcgaag
      541 cctgcgatgg agaactcgtc cttcgggagt tcgcccgtac cgccgaatcc gggcagcgag
      601 ggcgcgtaca cccggcagcc ctgcgccgcg acgcgcgaga cgacgctgcg gtacgtgtgg
      661 ttggccagcc cccacccgtg caggaagatg gnnnnnnnnn nccggcatgc cttcgcccgc
      721 aacgctgtag gcggctggac gatcgttgac gaaggttcgc ttctggctga tcggcgccag
      781 agtcgccggc gactgcgctc ctcccgcttg cgtcgcggcg tgctcaggca gagcgggaga
      841 tgcgttcgcg gcgttcgaac tcattcagtc cgccccacac gccatgaggt tcctcggtgc
      901 gcagcgcctg ttgcaggcac gcgtggctga ccgggcattg agcacagatc gactttgcca
      961 cagcttcccg ggcccggcga agctccttgc gttcgaagaa tggcggcgga tagaagatcg
     1021 ccgggtcttg cccccggcac gcggcgtcga gctgccaatc cggatcgtca ctgtcaactg
     1081 aacggctcat cgccacgggc acgtttcccc ttgctgcgtt gcgtgctgat cgggctcccg
     1141 ctgctgtcac agcacgtcgt tagtcaacct taacaaacca ccccggctgg gttcaatgcc
     1201 cagcctcggc ttccgcggta tcggcgtcga tgccggtggc gtctgtctct gcgtccgggg
     1261 tggcgccatg ggcgttctgc ttgcggttgc gcatctcgcg gtagtccttc gcgccgcctt
     1321 cttccggctc gacctcgagg gtcacgccgt cgatcgcgac cacgcgcagc acgtcgccgt
     1381 gctgcaacgg tgtggcccgg ttgacccgtg ctcgccagcg tccgccggca acttcggcca
     1441 tgccttcagg gtcgatcgcc ccgacggcct tgcccatctc gccgatcatc cactcgcgcc
     1501 cgaccgtggg ggtcgcgaag cgggtgcgca ccatcgacgg catcccgacc acgaacgaca
     1561 aggcgatgcc gatgatgccc gtgagcagcg tgatccacga cggtcgcacc gtggtgccga
     1621 gcacgtcggc gaacaagaac cacgacccga tcacgaacgc agccatgccg atgcccgtcc
     1681 acaatcgggg gacgccgacc tgcacgtcga tcgcgaacgc gaacatcgag aacagcagca
     1741 ccgccaccgc ccacccgcgc accggcaggg cagccaggcc gtagcaggcg agcagcgtgc
     1801 acaccgcccc gacgacgccg gcgatgccca cgccagccgt gaagaactcg aagatcagca
     1861 gggtcaggcc gatggcgaac agcagatagg ccaccgccgg gctggcgacg gtgtgcagca
     1921 gctggttgac cagaccgagc ttggtgaagc gcagcagggt ggcgttgatc tgcgtgccgc
     1981 cgtcggcgtt gagcgactcg gaccccgggc ggtgagcccg tcgagggcga gggccatgtt
     2041 cctgacggcg ggcacgcctt cgtcggtgat gctcagcttg agcgccccgg cccggcgagc
     2101 ctcttgaaaa cccatcgact cgctgcgcag ccgctcggtg gcagcgccga agtcgaagct
     2161 gacgttggcc accggcaagg gcgcgcccat cttgccgatg tgggtaccgg gcgccatcgc
     2221 ggtggcgtcg gcagcggcca tcatctgcat cccgagcccg gtcagcttgg ccccggacgg
     2281 cccgacccac accccgatcg ggatcggcgc gtcgcgcaac gtctcgacca gatcggtcat
     2341 tcggtcacgg ccgacgaccg cgccgggtga gttgacctgc aggatcaggg cctggctgcc
     2401 gttggccacg gcgtcgcgga tcgcgacctc gatctcgtcg atgaggatct cgtcgatcag
     2461 gccgctgacc tgcacgacgt cgaccggagc gaggtgatcg accgagtcgt cactggggtc
     2521 gaccggggct gccattgggg ccgctgagcg agcggacggg gacgccgtgg cgggctcggc
     2581 gttggccgcc gattcgccga ttccggacag aagggtggcg acggccgcgc tcgccaagag
     2641 ggtgatcgct agtctgcgca aggaaatggc ctccggaggg cgtgtggaaa ctcaacaagt
     2701 cttcggcgcg gcccggctgg tgatcgtggc tggcaagggc ggcgtcggca agacaacggt
     2761 aaccgcagtc atcgctcacg cggcagccgc tgccggactt cgggtcgcgg cgatcgatct
     2821 ggagggcaag ccggcgcttg ccaacatgct cgccgacagc cccgtcgagg tcaccacgat
     2881 caccgcgtcc gacgcactgg gcgagtacct cgacacccac gggctcggcc gggtcgcgaa
     2941 gcgactggcc aacagcgggg tgatcgaggt ggtcgccacc gccgcgcccg gcatcgacga
     3001 catcgtcgtg ctcggcaagc tgaagcaact cgaacgatcc ggcgagtacg atctggtggt
     3061 ggtcgacggg ccagccgctg ggcacgcgat cacgttcctg ctctcccccg ccggattgct
     3121 ggagacagtg cgcagcggcc cgatccgggt ccaggctcag gacgtgctgg agatgttcgc
     3181 cgatcctcag cgttgccagg tcgtgctggt cggcctgccg gaagcgacgc cgatcaacga
     3241 gttgatcgaa accgcctaca tgatcgagga gcgggtcggc gtccagctcg gaccggtggt
     3301 gatgaacggc gtcgatcccg gaccagaggt ggtgctgagc ggcaccggcg atctcagcgc
     3361 ggacgatccg ttgatcgctg cggccgagtt ccgcaaccat cgcttggcgt ggcagcgcgg
     3421 cgagctcgaa cgcctggccg gggagttggc cctcgaccgc tacgtactgc cccaacttcc
     3481 ggtcgcgggc atctccgctg ccgacgtggc cacgctggcc gcagcactcg tcgcccaact
     3541 cggtgacagg gcgtgggatg ccccgaggcc gcagactccg gtggccccgg tcacccgatg
     3601 agtcgctcca agcccccgaa acc
//
LOCUS       DAORNF010000305         3595 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_19519_length_3595_cov_1.524294, whole genome shotgun sequence.
ACCESSION   DAORNF010000305 DAORNF010000000
VERSION     DAORNF010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3595
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19519_length_3595_cov_1.524294"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            64..>762
                     /locus_tag="PLV68_12280"
     CDS             64..>762
                     /locus_tag="PLV68_12280"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRE02070.1"
                     /translation="MGGEVPLSDPIAVTFHGVRGSTPCNSPEITRYGGNTSCVSLDVP
                     GHDPILFDMGTGMRYYGAQQRATGRAFRGSVLLTHMHWDHTQGLPFFTPIQCADGELT
                     IYGPGQDDGRSVGDVLADIIKPPLFPVPISAFPGRLHFRDMADDDFVLSSSGPGGPDG
                     GDVTVMSRLIPHIGRTCGYRVTWAGRSVTYMSDHQMPRDGSFTASDSALELAAGTDLL
                     IHDSQYTDAEFAHKW"
     assembly_gap    763..875
                     /estimated_length=113
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            974..1339
                     /locus_tag="PLV68_12285"
     CDS             974..1339
                     /locus_tag="PLV68_12285"
                     /inference="COORDINATES: protein motif:HMM:NF018942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HRE02071.1"
                     /translation="MASVVVGLVLLVSGGTKVAAGAAWPNQARQLGAPRWAIAPLPWV
                     EIVVGAAACAQLVRPWPAVAAAALFVAFTALIVMRLREGRHPPCACFGSWSAKPLGWG
                     HVGRNAVFLAVAVGAIVAA"
     assembly_gap    1350..1384
                     /estimated_length=35
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1385..>1716)
                     /locus_tag="PLV68_12290"
     CDS             complement(<1385..>1716)
                     /locus_tag="PLV68_12290"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HRE02072.1"
                     /translation="AAAVAEVPFVDIVSTMSDPSLPLTVTEWEEWGDPRSEPAASIIL
                     AYSPYDNTVAADYPAMYVTAGLNDPRVSYHEPAKWVARLREVWRTDRPLVFKCEMGAG
                     HAGPSGRY"
     assembly_gap    1717..1863
                     /estimated_length=147
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1864..>2136)
                     /locus_tag="PLV68_12295"
     CDS             complement(<1864..>2136)
                     /locus_tag="PLV68_12295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HRE02073.1"
                     /translation="SRYTAHREWATAPDGTQVPVDIVRCVDTPAGSGAPCLVYGYGSY
                     EISMPPWFSVGRFSLLDRGWTWALVHPRGGGELGRRWYLDGKLEHKI"
     assembly_gap    2137..2146
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2147..>2558)
                     /locus_tag="PLV68_12300"
     CDS             complement(<2147..>2558)
                     /locus_tag="PLV68_12300"
                     /inference="COORDINATES: protein motif:HMM:NF014896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02074.1"
                     /translation="DVEYHLDHWGDRFVVVTNLGAEDFRVMTASIAEPGTWTEFVAHE
                     PGRRITGAEPFADHLVLHEWHHAQQRVRILFRDGSERVVDLGDEPHEVELDANPEWAT
                     DTVRFSYQSFTTPPTVYEEHVRTGARELLKQTPAP"
     assembly_gap    2559..2575
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2576..>3255)
                     /locus_tag="PLV68_12305"
     CDS             complement(<2576..>3255)
                     /locus_tag="PLV68_12305"
                     /inference="COORDINATES: protein motif:HMM:NF014896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02075.1"
                     /translation="TIAYLDAENAYADAWFAQHEATVETVFAEIKSRVRETDAAAPVR
                     KGDWWYTTRMVEGQSYAIHCRGRSRDTAEDTVLIDENELAAGHDFFDMGVFDVSPDHA
                     LAAWSGDTDGGERYVMRIRDLATGADLGDELANTSWGGSAWVADASHLFYVQSDEAMR
                     PYQVWRHRLGTPQTDDVMVFEEPDERFFVSVDLTRSGEWVVIDVGSKTSSEVRLIRAT
                     DPTAEPTL"
     assembly_gap    3256..3297
                     /estimated_length=42
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3470..>3595
                     /locus_tag="PLV68_12310"
     CDS             3470..>3595
                     /locus_tag="PLV68_12310"
                     /inference="COORDINATES: protein motif:HMM:NF024851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HRE02076.1"
                     /translation="MRVWIDQDLCTGDGLCLDHCPDVFVQLEDGISYVAEFGVALN"
BASE COUNT          458 a         1100 c         1122 g          551 t
ORIGIN      
        1 gcgaccaacg tcacagaagt tggctgtgta cttgcgccgg gtcggtggcc tagcgtgaag
       61 cccttggggg gtgaagtgcc gctatccgat ccgattgcag tgacgttcca cggggtgcgc
      121 gggtcgacac cgtgcaacag tcctgagatc acccgctacg gcggcaacac gtcgtgcgtg
      181 tcgctcgacg ttcccggtca cgaccccatc ctgttcgaca tgggcaccgg tatgcgctac
      241 tacggggcgc agcagcgggc gactggccgg gccttccggg gttcggtgct gctcacccac
      301 atgcactggg accacaccca aggattgccg ttcttcacgc cgatccaatg cgccgacggc
      361 gaactcacca tctacggacc cggtcaagac gatgggcgca gcgtcggcga cgtgttggcc
      421 gacatcatca agccaccgtt gttcccggtg cccatcagcg cattccctgg ccggctccac
      481 ttccgcgaca tggccgacga cgacttcgtg ctgtccagct ccggccctgg tggccccgac
      541 gggggcgacg tcaccgtcat gtcacggctc atcccgcaca tcgggcgcac ctgcgggtac
      601 cgggtcacct gggccgggcg atcggtcacc tacatgagcg accatcagat gccccgtgac
      661 ggcagcttca ccgcatccga ctcggcgctc gagctcgcgg ccggaaccga cctgttgatc
      721 cacgactcgc agtacaccga cgccgagttc gcccacaagt ggnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncgccg cagcgaaggc cccgggcgcg
      901 gcccgcggcg tcgaggtgat cgcggcccgc gagggcctca ccgtgcaagt gtgatgccgt
      961 gacgctgacc gcggtggcgt ccgtcgtcgt cggtctggtg ctgctcgtgt cgggcggcac
     1021 caaggtcgct gctggcgcgg catggccgaa ccaggcccgt cagctcggtg caccgcgatg
     1081 ggcgatcgcg ccgttgccgt gggtggagat cgtggtcggg gccgcggcct gcgcccaact
     1141 ggttcgtccc tggcctgcgg tggcggcggc ggcattgttc gtcgccttca ccgcgttgat
     1201 cgtgatgcgt ctgcgcgaag gccgtcatcc tccgtgcgcc tgcttcggca gctggagcgc
     1261 gaagccgctc ggctggggcc acgtcgggcg caacgcggtg ttcctggccg tcgccgtcgg
     1321 ggcgatcgtc gccgcctgac cggccagcgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnntgtagc gcccgctggg cccagcgtgg cccgcaccca tctcgcactt gaacaccagc
     1441 ggccgatcgg tgcgccacac ctcgcgcagc ctggcgaccc acttggccgg ctcgtggtag
     1501 ctgacccgcg ggtcgttcag cccagcggtc acgtacatcg ccgggtagtc ggccgcgacg
     1561 gtgttgtcgt acggcgaata ggccaggatg atgctcgccg caggctcgct gcgcggatcg
     1621 ccccactcct cccattcggt gaccgtcagc ggcagcgacg gatcgctcat ggtcgacacg
     1681 atgtcgacga acggcacctc ggccaccgcg gccgcgnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnngatcttg tgttcgagct tgccgtcgag gtaccagcgc cgccccaact cgccgcctcc
     1921 acggggatgc accagcgccc acgtccagcc gcggtcgagc agtgagaagc gccccaccga
     1981 gaaccacggc ggcatgctga tctcgtagct gccatagccg tagaccaggc acggagcacc
     2041 tgacccggcc ggggtgtcga cgcagcggac gatgtcgacg ggtacctggg tgccgtcggg
     2101 tgcggtggcc cattcgcggt gggccgtgta gcggctnnnn nnnnnngggg gccggggtct
     2161 gcttgagcag ttcgcgagcc ccggtgcgga cgtgctcctc gtacaccgtc ggcggcgtgg
     2221 tgaacgactg gtagctgaac cgcaccgtgt cggtggccca ctcggggttg gcgtcgagct
     2281 cgacctcgtg cggctcgtcg cccaagtcga ccacccgctc gctgccgtcg cggaacagga
     2341 ttcgcacccg ctgttgggcg tggtgccact cgtgcagcac gaggtgatcg gcgaagggct
     2401 cggccccggt gatgcgccgt cccggttcgt gggcgacgaa ctcggtccag gtgcctggct
     2461 cggcgatcga cgcggtcatc acccggaagt cctcggcgcc gaggttggtg acgaccacga
     2521 agcggtcgcc ccagtggtcg aggtggtact ccacgtcgnn nnnnnnnnnn nnnnncaagt
     2581 gttggttcgg cggtcgggtc ggtggcgcgg atcaggcgca cttccgacga cgtcttgctg
     2641 ccgacgtcga tcacgaccca ttcgccgctg cgggtgaggt cgaccgacac gaagaagcgc
     2701 tcgtcgggct cttcgaacac catcacgtcg tcggtttggg gggtacccaa ccggtggcgc
     2761 cacacctggt aggggcgcat cgcctcgtcg gactggacgt agaacaggtg gctggcatcg
     2821 gccacccacg ccgacccgcc ccacgacgtg ttggccagtt cgtcgccgag gtcggccccg
     2881 gttgccaggt cgcggatgcg catcacgtag cgctcaccgc cgtcggtgtc gcccgaccat
     2941 gcggccaacg cgtggtcggg gctgacgtcg aacacgccca tgtcgaagaa gtcgtggcct
     3001 gcagccagtt cgttctcgtc gatcagcacc gtgtcctcgg ccgtgtcacg tgagcggccg
     3061 cggcagtgga tcgcgtagct ctggccttcg accatccgcg tcgtgtacca ccagtcgccc
     3121 tttcgcaccg gggccgcggc gtcggtctca cggacgcgcg acttgatctc cgcgaacacc
     3181 gtctcgaccg ttgcctcgtg ctgggcgaac caggcgtcgg cgtaggcgtt ctcggcgtcg
     3241 agataggcga tggtgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncgg
     3301 tgtcaccggt cgggcggtgc cacgtgtgcg ccacctgctt ggcgacaggg gcagagacgg
     3361 gggaggcgtt tggatcggtg ccggtcacgc cgtgcagcgt acgcagccgc aactcgtcat
     3421 gccgggcgaa ggccgcgcct ggggatggca ccgatagctt cagcagcgga tgcgcgtctg
     3481 gatcgatcaa gacctgtgca cgggtgatgg gctctgtctc gaccactgtc ccgatgtgtt
     3541 cgtgcaactg gaggacggca tctcctacgt ggccgagttc ggggtcgcgc tcaac
//
LOCUS       DAORNF010000306         3583 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_19611_length_3583_cov_1.738095, whole genome shotgun sequence.
ACCESSION   DAORNF010000306 DAORNF010000000
VERSION     DAORNF010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3583
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19611_length_3583_cov_1.738095"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="PLV68_12315"
     CDS             complement(<1..244)
                     /locus_tag="PLV68_12315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02077.1"
                     /translation="MSEEIESEEITADEIGNADRLTAPTPTTDTGAEATVDTGAEATV
                     DAAPVDAASNDDPDDDSDNEHEGDPSESAEDEVDDDD"
     assembly_gap    371..393
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<394..1650)
                     /locus_tag="PLV68_12320"
     CDS             complement(<394..1650)
                     /locus_tag="PLV68_12320"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRE02078.1"
                     /translation="MTTPFADLGLPARLVESLAADGITDAFPVQAATLPDALAGRDLC
                     CRAPTGSGKTLAFGIPLMVRIGQAEPSKPTGLVLVPTRELAEQVQRAFVPLGRAQWRF
                     VQAIFGGTSFGPQIHSLRKGASVVVATPGRLLDIIDQGYCDLSAVEVCVIDEADRMAD
                     LGFMPQLRAVLDLTPTTRQTMLWSATLDGDVSELVRDYLRDPVVHDLVGDAADVEHRV
                     IVTHPDDRVAAAAELIEQHGPTVVFVTTRDGVDDVSEALGRAGVSAGALHGARSQAVR
                     QRVLDDFKRGRIQALVATDVAARGLHVDNVSLVLHYDLPASEKDYTHRSGRTGRAGNA
                     GTVIAFVTPGREHLATRLRDGGDAASWDDDEWGNDDWGDQWNDDSWNDNAWTANSWDS
                     ATSGAASWQDTSDRHDDRADDERASDD"
     gene            complement(1954..>3583)
                     /locus_tag="PLV68_12325"
     CDS             complement(1954..>3583)
                     /locus_tag="PLV68_12325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HRE02079.1"
                     /translation="PVVAAGVALDQPAMVLHANRVIARTPAATARGVLIGHRRRDAQR
                     ACPLVRIVDHDPARDARAFEAAVRSIAAMVPRLETTEPGLLTFPAKGPARYFGGEHAM
                     AGRVGDLLVAAIPSLATVSATGVSTGIGLGIGDGRFTAGVAARRSVRAMRAGRDISPV
                     VVDAGQRAAAAFLAPLGVDLLRDVAGVDPAVVDLFRRLGIRTLGARAALPASEVFGRF
                     GHAGAFAHRVAGGGDDRPPGAERPSPDWVVAQHFDNPVTLIDPLVFAAKQLAASLLAR
                     LRANGMVCTRLTVAAVTDGGERTEHAWYRPHGLSAAAIVERVRWQITGWIRSGRAGAA
                     TAGAPTSGVVSIELIPDEVCADAGVQLGFWGGRSVADDWAARAVARVVGLVGGDQVLV
                     PAAQGGRHPEDTYRWVPVEQTDLTEPAQRLAPANTGHGATERAPWWGQLPAPSPATVL
                     AEALPVELVDRSGVTVRVDGRGGLHGEPAVLHHPRHGALSVVTWAGPWPVDERWWDPV
                     HQRRLARLQIVTRDGTAALVVAEHRRWWITALYT"
BASE COUNT          503 a         1210 c         1287 g          560 t
ORIGIN      
        1 tgtcgtcgtc gtcgacctcg tcttcggcac tctctgaggg gtcgccctca tgctcgttgt
       61 cggagtcatc gtcggggtcg tcgttgctgg cggcgtcgac gggggccgcg tcgacggtcg
      121 cttcggctcc ggtatcgacg gtcgcttcgg ctcccgtgtc ggtggttggg gtcggcgcgg
      181 tcaggcgatc ggcgttgccg atctcgtcgg cggtgatttc ttcggattca atttcttctg
      241 acatagttcc ttcttcaaga cgtgcttcgt cgttgttgtg ttcgtcgtgc tggtgttcgg
      301 cccgtgtcgt ggccgggcgg cggatggtgg tggtgcgttt cggggtgccg gcggggacga
      361 gcgatgggtc nnnnnnnnnn nnnnnnnnnn nnnatcgtcg ctggctcgtt catcgtcggc
      421 gcggtcgtcg tgtcggtcgc tggtgtcctg ccacgacgca gcccccgacg tggcgctgtc
      481 ccatgagttg gcggtccatg cgttgtcgtt ccacgaatcg tcgttccact ggtcgcccca
      541 gtcgtcgttg ccccactcgt cgtcgtccca ggaggccgcg tcgccgccat cgcgcaggcg
      601 ggtggcaagg tgctcgcgcc cgggggtgac gaaggcgatc acggtgcccg cgttgccggc
      661 ccggccagtg cgcccggagc ggtgggtgta atccttctcg ctggccggca ggtcgtagtg
      721 cagcaccagg ctgacgttgt cgacatgaag gccacgggcg gccacgtcgg tggcgaccaa
      781 tgcctggatg cggccccgct tgaagtcgtc gagcacccgc tggcgcaccg cctggctgcg
      841 tgccccgtgc agcgcccccg cactgacccc agcccgcccg agagcctcgc tgacgtcgtc
      901 cacgccatcg cgggtggtga cgaacacgac cgtcgggccg tgttgctcga tcagctctgc
      961 cgccgccgcg acgcgatcgt cggggtgggt gacgatgacg cggtgctcga cgtctgcagc
     1021 gtcgccgacc aggtcgtgca ccacgggatc gcgcaggtag tcgcggacca actcgctgac
     1081 gtcgccgtcg agggtggccg accacagcat ggtctgacgg gtggtgggtg tgagatcgag
     1141 tacggcccgc agctggggca tgaagccaag gtcggccatg cgatcggcct cgtcgatcac
     1201 gcacacctcg accgcgctga gatcgcaata gccctggtcg atgatgtcga gcaggcgtcc
     1261 cggtgtggcg accacgaccg aggccccctt gcgcagcgag tggatctggg ggccgaacga
     1321 ggtgcctccg aagatggcct gcacgaaccg ccactgggcc cgtccgagtg ggacgaaggc
     1381 ccgctgcacc tgctcggcca gctcgcgggt gggaaccagc accaggccgg tgggtttgct
     1441 cggttcggcc tggccgatgc ggaccatcaa ggggatgccg aacgcgaggg tcttgcccga
     1501 gccggtgggg gctcggcaac acagatcgcg gccggccagt gcatcgggca gggtggccgc
     1561 ctggacgggg aatgcatcgg tgatgccgtc ggccgcgagc gactccacca aacgcgcggg
     1621 gaggccgagg tcggcgaacg gggtggtcat gttgtcgtag ctccttgcac agacccggac
     1681 tcttgcgggt tctccgaatc gccctcgtgg cggtgacggt ctgagcccgg gtttactgag
     1741 gccttaaggc aggtcgagct cgacgttgac agcactgttc acctggaaca ggcacgacga
     1801 cggtatcgcc cttggctctc aacggcaaca acgctggcgg tactccgtcg gtgaatccag
     1861 cttccgcgga gtacggatgt gtcggattcc gggcattgcc cgctccgcgg acgcccgctt
     1921 cgccggtgcg gggtggcgtc tgtcgtcggc aggtcaggtg tagagggcgg tgatccacca
     1981 gcggcggtgt tcggcgacga cgagcgcggc ggtgccgtcg cgggtgacga tctgcaaccg
     2041 ggccaggcgc cgctggtgaa ccggatccca ccagcgttcg tcgaccggcc acggaccggc
     2101 ccaggtgacc accgagagtg caccgtggcg tgggtggtgc aacaccgccg gctcaccgtg
     2161 cagaccgccg cggccgtcga ctcgcacggt gacgccagag cgatcgacca gttccaccgg
     2221 gagagcctcg gccagcacgg tggccggtga gggggccggc aactgccccc accacggcgc
     2281 tcgctcggtg gcgccgtgac cggtattggc aggggccagc cgctgggctg gctcggtcag
     2341 gtcggtctgt tcgaccggta cccagcgata ggtgtcttcg gggtggcgcc caccctgcgc
     2401 ggcaggtacc agaacctggt cacccccgac cagaccgacc acgcgcgcga ccgctcgcgc
     2461 agcccagtcg tcggcgacgc tgcggccgcc ccagaacccg agctgcaccc cagcgtcggc
     2521 acacacctcg tccgggatca actcgatcga cacgacaccg ctggtagggg caccagcggt
     2581 tgcggcaccg gcccggccag agcgaatcca cccggtgatc tgccagcgca cccgttcgac
     2641 gatggccgcc gcgctcaacc cgtggggccg ataccacgca tgctcggtgc gttctccgcc
     2701 gtcggtcacc gcggccaccg tcagccgggt gcacaccatg ccgttggcac gcaacctggc
     2761 gagcaacgat gcggccagtt gcttggccgc gaacaccagg gggtcgatca gggtgaccgg
     2821 attgtcgaag tgttgggcca ccacccagtc gggcgacggc cgctcggcac cgggtgggcg
     2881 gtcgtcgccg ccacccgcca cgcggtgagc gaaggcaccc gcgtggccga accggccgaa
     2941 cacctcggac gcggggagtg ccgcccgcgc gccgagggtg cggatgccga ggcggcggaa
     3001 caggtcgacg accgcaggat cgaccccggc aacatcgcgc aacagatcga ccccgagggg
     3061 agcaaggaac gccgccgcgg cgcgttggcc cgcgtcgacc acgacggggc tgatgtcacg
     3121 cccggctctc atcgctcgca ccgaacggcg cgccgccaca ccggcggtga agcgaccgtc
     3181 gccgatgccc agaccgatcc ccgtgctgac gcctgttgcg ctgacagtcg ccagggaagg
     3241 gatggctgcg acgagcaggt cgccaacccg cccggccatg gcgtgctcgc ctccgaagta
     3301 ccgcgctggc cccttggcgg gaaaggtcaa cagcccgggc tcggtggtct ccaaccgcgg
     3361 caccatggcg gcgatgctgc ggacagcggc ctcgaaggcg cgggcgtcgc gggccgggtc
     3421 gtggtcgacg atgcgcacca gcggacatgc ccgctgcgca tcccggcgcc ggtgcccgat
     3481 cagcacccct cgggcggttg cagcgggagt acgggcgatg acccggttgg cgtggagcac
     3541 catcgccggt tggtcgagcg cgacccccgc cgccaccacc ggc
//
LOCUS       DAORNF010000307         3577 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_19656_length_3577_cov_1.894094, whole genome shotgun sequence.
ACCESSION   DAORNF010000307 DAORNF010000000
VERSION     DAORNF010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3577
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19656_length_3577_cov_1.894094"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="PLV68_12330"
     CDS             <1..558
                     /locus_tag="PLV68_12330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02080.1"
                     /translation="GLGLTAAGFGAWSERLVAAMSEHQPSIVVVVVGANDAAGFVSPP
                     IDEVGSLAWRTEYRRRVGVVVDIATAGGARLIWVGAANVTDPALVAPIAVQNEVVAAA
                     VADRTGTIFVDAWRRFSTLIPAGDESSIALAGEVFDDRDGTTKAVRDSDETGTTLNRA
                     GQRILAALIADAVDTALVALGADLP"
     gene            721..1536
                     /locus_tag="PLV68_12335"
     CDS             721..1536
                     /locus_tag="PLV68_12335"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HRE02081.1"
                     /translation="MTDTPIAPAADVVLLDVSERIATITLNRPEARNALSSAVLRRLP
                     QVVREAEARDDVDVIILTGADPAFCAGLDLGELGDSGTNLGAGASERGSRPGEDGRRV
                     RGPFPPLTKPLIGAINGAAVTGGFELALNCDFLIASERAKFGDTHSRVGVMPGWGLTV
                     LLPQAIGVRRAREMSFTGNFMLADEALRCGLVNRVVAHDDLLPTTRQIARDIIGNEQP
                     GVRQIRATYAEITGDESGWQIEARDSKAWQATTFTTEAVAARRDAIMSRGRTQ"
     gene            1685..>2064
                     /locus_tag="PLV68_12340"
     CDS             1685..>2064
                     /locus_tag="PLV68_12340"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRE02082.1"
                     /translation="MSLVDVSISEGVATLTLNNPAERNTLTAPMVAEILAAMDAIEAD
                     DGVGALVVTGAAPAFCAGANLGNLAEASGDSLGTIYAGFLRIADSPLPTIAAVNGAAA
                     GGGFALVLGSDVRLAARAAKFNAA"
     assembly_gap    2065..2147
                     /estimated_length=83
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2148..2441
                     /locus_tag="PLV68_12345"
     CDS             <2148..2441
                     /locus_tag="PLV68_12345"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442912.1"
                     /note="Catalyzes the reversible hydration of unsaturated
                     fatty acyl-CoA to beta-hydroxyacyl-CoA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HRE02083.1"
                     /translation="VVFGEVLDGAEAERVGLVHRCVDDDRLLAAAQEMAARAASAPRE
                     LVIETKRTIRDMADVSVHADAVTRELEPQLWSTRQPWFADKIAALQKKISSKS"
     gene            2577..2894
                     /locus_tag="PLV68_12350"
     CDS             2577..2894
                     /locus_tag="PLV68_12350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02084.1"
                     /translation="MSQRHHMQETAPPPKMDLRAKAHNERHRINSELSAVAAQVSSGL
                     EPDDAHEPGPAWVPLKRHDAERAKEDAAKKRPRHWKMKMWKRRSATRRERALTSRLVA
                     EQA"
     gene            complement(2953..>3224)
                     /locus_tag="PLV68_12355"
     CDS             complement(2953..>3224)
                     /locus_tag="PLV68_12355"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HRE02085.1"
                     /translation="KAGHGYIAEGVAVVLRFAFDDLHLHRVEICIIPRNANSRRVMDK
                     LRIREEGVAQRFLEIDGVWEDHMRYGITAEEWHERRNELTTGWLG"
     assembly_gap    3225..3366
                     /estimated_length=142
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3367..>3577)
                     /locus_tag="PLV68_12360"
     CDS             complement(<3367..>3577)
                     /locus_tag="PLV68_12360"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HRE02086.1"
                     /translation="PSLSGTGRSGLRLYGKRVVLRPLVAHDFPSWSEVRTRNEAWLLP
                     WEPKRPTHLGDPARDRDVFASRCSAP"
BASE COUNT          535 a         1168 c         1154 g          495 t
ORIGIN      
        1 ggcttgggac tcacggcggc cgggtttggg gcgtggtccg agcgcctggt ggcggcgatg
       61 tccgagcacc agccgtcgat cgtggtggtg gtcgtcgggg ccaacgacgc cgccgggttc
      121 gtctctccgc cgattgacga ggtcggctcg ctggcctggc gcaccgagta ccgccgccgg
      181 gtcggtgtcg tggtcgacat cgccaccgcg ggcggggcac ggctgatctg ggtcggggcc
      241 gccaacgtca ccgacccggc actggtcgcg ccgatcgcgg tccagaacga ggtcgtcgcc
      301 gcggcggtgg ccgaccgtac cggcacgatc ttcgtcgacg cctggcgccg gttctcgacg
      361 ttgatccctg ctggggatga atcctcgatc gcgctggccg gcgaggtgtt cgacgatcgc
      421 gacggcacga ccaaggccgt gcgcgacagc gacgagacgg gcacgacact gaatcgcgcc
      481 ggtcaacgca tcctcgcggc cctcatcgcc gacgccgtcg acaccgccct ggtcgccctc
      541 ggcgccgacc tgccctgact caccgccctc atccgcctcg ccctccgcgg cacgtcccgc
      601 gtccgcggat cggggattgc ccggatcccg gcaactccgc tgaagccaga ttcgccggcg
      661 cggcgattcg cctgggcgcg gactgccgga ttcgccgcgc cggtcggtca tcatggagcc
      721 atgaccgaca cccccattgc ccccgcagcc gatgtcgtgc tgctcgacgt cagcgagcgg
      781 atcgccacca tcacgctcaa ccggcccgag gcgcgcaacg cgctcagcag cgcggtactc
      841 cggcggctgc cccaagtcgt gcgcgaagcc gaagcgcgcg acgacgtcga cgtgatcatc
      901 ctcaccggcg ccgatccggc gttctgcgcg ggcctcgacc tgggcgagct tggcgacagc
      961 ggcaccaacc tcggcgcggg tgcgagcgaa cgcggttcgc ggcctggcga ggacgggcgg
     1021 cgggtgcgtg gcccgttccc gcccctcacc aagccgctga tcggagcgat caacggtgcc
     1081 gcggtcacgg gtggcttcga actggcgctc aactgcgact tcctgattgc cagcgagcgg
     1141 gccaagttcg gtgacaccca cagccgggtc ggggtgatgc cgggttgggg tctgaccgtg
     1201 ctgttgcccc aggcgatcgg cgtgcgccgg gcgagggaga tgagcttcac cggcaacttc
     1261 atgctggccg acgaggcgct gcgctgcggc ctggtcaacc gcgtggtcgc ccacgacgat
     1321 ctgttgccca cgacgcgcca gatcgcgcgt gacatcatcg gcaacgaaca gccaggggtg
     1381 cgccagatcc gggcgaccta cgccgagatc accggcgacg aatccggctg gcagatcgaa
     1441 gcccgcgaca gcaaggcctg gcaggccacc accttcacca ccgaggccgt cgccgcccgc
     1501 cgcgacgcca tcatgtcccg cggtcgcacc cagtaacgcc ccgtcacgtc tttcaacacc
     1561 tcccggcaaa tccgggtccg cggagtgcgg acttgccgga aaccgggcaa tccccgatcc
     1621 gtggaggccg aattcgccgg ggcgaggaag ggagagcgcg ggtgggcgtg gaacaatcgt
     1681 ccccatgagt cttgtcgatg tgtcgatctc cgagggtgtg gccaccctca cgctgaacaa
     1741 tcccgccgag cgcaacacgc tcaccgcccc gatggtcgcc gagatcctcg ccgcgatgga
     1801 tgcgatcgaa gccgacgacg gggtcggtgc gctggtggtc accggtgctg ctccggcgtt
     1861 ttgcgctggc gccaacctgg gcaacctggc cgaagccagc ggcgacagcc tgggcacgat
     1921 ctacgccggg ttcttgcgca tcgccgacag cccgctcccc acgatcgccg cggtcaacgg
     1981 tgcggcagcc ggtggcggat tcgcgttggt gctcggcagc gacgtccggc tggcggcccg
     2041 tgcggcgaag ttcaacgcgg cgttnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngtg gtcttcggcg
     2161 aggtgctcga cggtgccgaa gccgaacggg tcggactcgt ccaccgctgc gtcgacgacg
     2221 accgcctgct cgccgcggcc caagagatgg ccgcccgcgc ggcgagtgca ccgcgcgagt
     2281 tggtgatcga gaccaagcgg accatccgcg acatggccga cgtctcggtc cacgccgacg
     2341 cggttacccg cgagctcgaa ccgcaactgt ggagcacccg tcagccgtgg ttcgccgaca
     2401 agatcgccgc gctccagaag aagatctcct cgaagtcctg atctggtcag atcctcatcg
     2461 ctgtggggcg cgggcgtgac gaaatcggaa atctctggcg ttcgcgacgc ctcgacgcgc
     2521 gcggtggcag tgcaagggga gtgccatcaa ccgaactgca atgccaggag gtaccgatga
     2581 gtcaacgcca tcacatgcaa gagacggctc cgccgccgaa gatggacttg cgtgcgaaag
     2641 ctcacaacga gcggcaccgc atcaacagcg agttgagtgc ggtcgcagcc caggtgagct
     2701 cgggactcga acccgacgac gcccacgaac ctggcccggc gtgggtgccg ctcaagcgcc
     2761 acgacgccga gcgggccaag gaggacgcgg ccaagaagcg tccacggcac tggaagatga
     2821 agatgtggaa gcgtcgctcg gcgactcggc gcgaacgcgc cctcacatct cgcctggtcg
     2881 ccgagcaagc ctgaccaggc agccctgaac agttcgatgg ggcagggccc gtgatccctg
     2941 ccccatcgct ggtcagccca gccaacctgt ggtgagttcg ttgcggcgct cgtgccactc
     3001 ttcggcggtg atgccataac gcatgtggtc ttcccacacg ccgtcgatct ccaagaagcg
     3061 ttgtgccacg ccctcttcgc ggatgcgcaa cttgtccatc acccggcggc tgttggcgtt
     3121 gcgggggatg atgcagatct cgacgcggtg caggtgcaag tcgtcgaacg cgaagcgcag
     3181 caccaccgcg acgccctccg cgatgtagcc gtgtccggcc ttgtnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnggga gccgagcacc ggctggcgaa gacgtcgcga tcacgggcag gatcgccgag
     3421 gtgggtgggc cgcttcggct cccacggcag cagccacgcc tcgttgcgcg tgcgcacctc
     3481 gctccacgac gggaagtcgt gggccaccag cgggcgtagc acgactcgtt tgccgtagag
     3541 gcgcaggcca ctgcggccgg tgccggacag cgacggg
//
LOCUS       DAORNF010000308         3576 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_19668_length_3576_cov_1.495882, whole genome shotgun sequence.
ACCESSION   DAORNF010000308 DAORNF010000000
VERSION     DAORNF010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3576
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19668_length_3576_cov_1.495882"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..512
                     /locus_tag="PLV68_12365"
     CDS             <1..512
                     /locus_tag="PLV68_12365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HRE02087.1"
                     /translation="LSLDDPNDEAVALSGAAGFNTVLSLEVDGTRYPAIIKEVQRHPV
                     KRTVAHIDFMQVDMKEVITFSVPLRLTGEAKAVTAHNGLVDPAVDSIEVECTPGNLPT
                     EFEIDITDMQPGDVIRLADLTAPAGVTILGDPDTTVVTALHGVSAADLETDGGEAAAE
                     GEAGSDAAE"
     gene            589..1209
                     /gene="pth"
                     /locus_tag="PLV68_12370"
     CDS             589..1209
                     /gene="pth"
                     /locus_tag="PLV68_12370"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HRE02088.1"
                     /translation="MALFGRGERAERRGTPIEYLIVGLGNPGKEYARTRHNVGEEAVV
                     ALASRCGAALKAGKDRAMTAEVTIAAKRCVLAFPLTYMNESGQAVGALARRYAIADPT
                     RLVVVHDELDLAPAQVRVKVGGGLAGNNGLRSITQHLKTQDYVRVRIGVGKPPSKERG
                     AAHVLSRVSKAEREALDVAIAIAADAVEAIVASGPDAAMQRFNIRT"
     assembly_gap    1255..1453
                     /estimated_length=199
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1454..>1781
                     /locus_tag="PLV68_12375"
     CDS             <1454..>1781
                     /locus_tag="PLV68_12375"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRE02089.1"
                     /translation="AQYPVGYLVVLAALFTVSDFFSIPGGDAVVVGVYHSLIGALSVW
                     LVYLIGRAVFSRRVGMIAAGALAVFPSSVYLAATYSIEPTFIVLSLALAALVVTHDWS
                     TRPGRGR"
     assembly_gap    1782..1885
                     /estimated_length=104
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1886..>2098
                     /locus_tag="PLV68_12380"
     CDS             <1886..>2098
                     /locus_tag="PLV68_12380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02090.1"
                     /translation="RVTAVVVVGMIVIVAPWAIRNEVKIGAFIPFSTNLGDTMCMARF
                     PGSDSRFHWADHEWCADPALPAHIRSN"
     assembly_gap    2099..2147
                     /estimated_length=49
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2169..>2616
                     /locus_tag="PLV68_12385"
     CDS             2169..>2616
                     /locus_tag="PLV68_12385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02091.1"
                     /translation="MMRHDHESLGEATTVHAGSLGDGTIDVLARLADWTFYVTALTSV
                     VGLGLLGRRRLRKTRVQSMLLTAGYLLAIPLGLWGSVRFHAPLIPFIVLLAAFAVDAL
                     QRRVRGETTAGQLDAPGEADVVEQGPVVGDQQDGAPERVERNLELFD"
     assembly_gap    2617..2724
                     /estimated_length=108
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2725..>3576)
                     /locus_tag="PLV68_12390"
     CDS             complement(<2725..>3576)
                     /locus_tag="PLV68_12390"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRE02092.1"
                     /translation="DVFLLDEPTNDLDLDGLAQLERWVAQQQAGMVIVSHDREFLRRC
                     VTHVAELDEFTHRLSLYAGGWDSYLAERELASQHARERYDEYAAKRDALAGRAQRERE
                     WATQGLGRAKRDLSEKDKSIRSFKINQTEQLAGKAARTEKMIERLEAVDEPREAWQLH
                     LSFGETARSGSVVARLDDVVVQRGEFTLGPVSAEIGFGERIAITGHNGSGKSTLLGVI
                     LGRIEPDRGTAMLGPSVVVGELTQGRGQLADDATLLEGFMAATGMTIPDARTLLAKFG
                     IGATEVTR"
BASE COUNT          459 a         1096 c         1010 g          551 t
ORIGIN      
        1 cgctctccct cgacgatccc aacgacgaag ctgtcgccct cagcggagcg gccgggttca
       61 acaccgtctt gtcgctcgag gtcgacggca cccgctaccc ggcgatcatc aaagaggtgc
      121 agcgtcaccc cgtcaagcgc accgtcgccc acatcgactt catgcaggtc gacatgaagg
      181 aggtcatcac cttctcggtg ccgctgcgcc tcaccggcga agccaaggct gtcaccgcgc
      241 acaacggtct ggtcgatcct gcggtcgact cgatcgaggt cgagtgcacg cccggcaacc
      301 tgccgaccga gttcgagatc gacatcaccg acatgcagcc cggcgacgtg atccgtttgg
      361 ccgatctcac ggctccggcg ggcgtcacga tcctgggtga tcccgacacc accgtcgtca
      421 ccgccctgca cggcgtcagc gccgccgacc tcgagaccga tggtggcgag gccgctgccg
      481 agggcgaagc cgggtccgac gccgccgagt gagtcgctgc ggcagcgcat cgtgacgctg
      541 ctgcaccgcc accgccgcgc cctacaccgc tgacacggct gacccgctat ggctctgttc
      601 ggacgcggtg agcgcgccga gcgacgcggc acgccgatcg agtacctcat cgtcgggctg
      661 ggaaatcccg gcaaggagta cgcccgcacc cgccacaacg tcggtgaaga agccgtcgtc
      721 gcgctcgcat cgcggtgcgg ggcagcgctg aaggcgggca aggaccgggc gatgaccgcc
      781 gaggtcacca tcgccgccaa gcgctgcgtg ctggcgttcc cgctgacgta catgaacgaa
      841 tcgggccaag ccgtgggcgc cctcgcccgc cgctacgcca tagccgaccc cacccgtttg
      901 gtggtggtgc acgacgagct cgatctggcg cccgcacagg tgcgggtcaa ggtaggtggt
      961 ggccttgccg gcaacaacgg tctacgcagc atcacccaac acctcaagac gcaggattac
     1021 gtgcgcgtcc ggatcggcgt cggcaagccg ccgtcgaaag agcgcggtgc cgcccacgtg
     1081 ctgtcacggg tgtccaaggc cgagcgggaa gcactcgacg tcgcgatcgc catcgccgcc
     1141 gacgcggtcg aagccatcgt ggcctccggc cccgacgcag ccatgcagcg cttcaacatc
     1201 cgcacctgag cgttcacacc cacccggcta tgttgtgcgg cgctgtgttt gactnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnggcccag tatccagtcg gttacctggt cgtgctcgcg gcgctgttca
     1501 ccgtgtccga cttcttctcg atccccggcg gcgatgcggt cgtcgtcggt gtgtatcact
     1561 cgctcatcgg cgcgctctcg gtgtggctgg tgtacctgat cggcagagcc gtcttcagcc
     1621 gccgcgtcgg catgatcgcc gcgggggcgc tggccgtgtt ccccagttcg gtgtatctcg
     1681 cggcgacata ttcgatcgag ccgacgttca tcgtgctttc actcgccctc gccgcgctgg
     1741 tcgttaccca cgattggtcg acacggcccg gcagggggcg gnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnncgcgt cacggccgtg gtcgtcgtcg gcatgatcgt
     1921 catcgtcgcg ccatgggcca ttcgcaacga agtgaagatc ggtgccttca tccccttctc
     1981 caccaacctc ggcgacacga tgtgcatggc ccgcttcccc ggctccgact cccgcttcca
     2041 ctgggccgat cacgagtggt gcgccgatcc cgcgctgccg gcgcacatcc gcagcaacnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnatc gtcgtgcgct
     2161 ttcgggagat gatgcgccac gatcacgagt cgctgggtga ggccaccacg gtccatgccg
     2221 gttcgctcgg cgacggaacg atcgacgtcc tcgcccggct cgccgattgg acgttctacg
     2281 tcacggcgct gacctcggtg gtcggcctgg ggctgctcgg tcggcgccga ttgcgcaaaa
     2341 ccagggtgca gtcgatgttg ttgaccgccg gatatctgct cgcgataccg ctcgggctgt
     2401 ggggatcggt ccgcttccac gcgccgctca tccccttcat cgtgctcctc gccgcgttcg
     2461 cggtcgatgc gttacagcgt cgcgtccgcg gtgagacgac cgccggtcaa ctcgatgcgc
     2521 cgggtgaggc ggacgttgtc gagcaaggcc cggtcgtggg tgaccagcaa gacggtgccc
     2581 ctgaacgcgt cgagcgcaac ctcgagctgt tcgatcnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnccgggt gacctcggtg gcgccgatcc cgaacttggc
     2761 cagcaacgtg cgcgcgtcgg gaatggtcat gcccgtcgcg gccatgaacc cctccagcag
     2821 ggtcgcgtcg tccgcgagtt gaccgcggcc ttgggtcagt tcgccgacga cgacgcttgg
     2881 tccgagcatc gctgtcccgc ggtcgggttc gatgcggccg aggatcaccc cgagcagcgt
     2941 cgacttgccg ctgccgttgt gaccggtgat cgcgatccgc tcgccgaagc cgatctcggc
     3001 cgagaccgga ccgagggtga actcgccccg ctgtacgacc acgtcgtcca ggcgggcgac
     3061 gaccgacccg ctgcgtgccg tctcaccgaa gctgagatgc agctgccacg cctcgcgtgg
     3121 ctcgtcgacc gcctccaggc gttcgatcat cttctccgtt cgtgccgcct tgcccgccag
     3181 ctgctcggtc tggttgatct tgaagctgcg gatggacttg tccttttcgg acaggtcccg
     3241 cttggcccgg ccgaggccct gcgtggccca ctctcgctcc cgctgggcac gtccggccag
     3301 tgcgtcgcgc ttggccgcgt actcgtcgta gcgttcccgt gcgtgctggc tagccagctc
     3361 gcgctcggcc aggtagctgt cccaacctcc ggcgtagagg ctgagacggt gggtgaactc
     3421 gtccagttcg gccacatggg tgacgcagcg gcgcaggaac tcgcggtcgt ggctgacgat
     3481 caccatcccc gcttgctgtt gagcgaccca acgttcgagt tgggcgagac cgtcgaggtc
     3541 gagatcgttg gtcggttcgt ccagcaggaa cacgtc
//
LOCUS       DAORNF010000309         3575 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_19676_length_3575_cov_1.519886, whole genome shotgun sequence.
ACCESSION   DAORNF010000309 DAORNF010000000
VERSION     DAORNF010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3575
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19676_length_3575_cov_1.519886"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>280)
                     /locus_tag="PLV68_12395"
     CDS             complement(<1..>280)
                     /locus_tag="PLV68_12395"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRE02093.1"
                     /translation="SRLVLRLVEPTTGTVALGGVPLAEMPIAELRRRVAMIPQEVELF
                     TGTVRDNITLFDSAPSDAAVEASLRAVGLEALADNGIHRALGPGGAGLS"
     assembly_gap    281..356
                     /estimated_length=76
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<357..>749)
                     /locus_tag="PLV68_12400"
     CDS             complement(<357..>749)
                     /locus_tag="PLV68_12400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02094.1"
                     /translation="MYRFVGESADALRAAVSRERAFLRVWWAVEAVVAMGSVVALALS
                     AVLVSRDVISLGTAFLMFQYMTLLSRPLNDMVDQLETVQKANGAIVRVIDLGAIRSSI
                     SDTGTRMPPPGPLSIEFRHVGFHYGDDA"
     assembly_gap    750..896
                     /estimated_length=147
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<897..1177)
                     /locus_tag="PLV68_12405"
     CDS             complement(<897..1177)
                     /locus_tag="PLV68_12405"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HRE02095.1"
                     /translation="MVVAWFATVTAWRTTNTIRLDTTRHVLGLDHEFHRTHTPGELIQ
                     RVDGDVTAVSDFLGSVVPKAAGSALLVLGVVVVLAVLDWRLAVGTLVYVV"
     assembly_gap    1209..1364
                     /estimated_length=156
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1549..>2080)
                     /locus_tag="PLV68_12410"
     CDS             complement(1549..>2080)
                     /locus_tag="PLV68_12410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02096.1"
                     /translation="YDTFAQWREATQRYQAMLLRHHIETLRRLKYRPTGGLAMFMLND
                     ASPAISWSVLDHLRQPKMGYFAVTEACRPVIVVADRLPAALAPGDKLAVDVHIVNDLR
                     VPVTDATLRAHLRWSNGEHEWSYTGTAEADSVTRIAVLQFVVPDAPGDLWLDLTLEAD
                     NVATTNRDRTVIVPPI"
     assembly_gap    2081..2093
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2094..>3575)
                     /locus_tag="PLV68_12415"
     CDS             complement(<2094..>3575)
                     /locus_tag="PLV68_12415"
                     /inference="COORDINATES: protein motif:HMM:NF012906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02097.1"
                     /translation="WRDSDEFATSDGPLLLRRRFEFPAPADGERAWVVLGGVFYQADL
                     WFDGAYIGDPEGYFFPHSFDVTSLARLADEHVLAVEVACAPHRSHKGRRNITGVFQQW
                     HAADRSWNPGGLWRPVTVERTGPVRLDRLRVLCRDANDTRAHLRLHARLDSDEPRQVR
                     IRTTVTVPGRRSEVLSEFDQSLARGANAVGWNLDVANPTLWWPWSLGDQPLTQVDVEV
                     LADGVVSHGRSTRTGLREVAMQDWIFSVNGERLFLKGANLAPTRLDIANATDADFRRD
                     IELARDAGLDMLRVHGHISAQPLYDAADELGMLLWQDFPLQWSYAREVRRRAVAQADE
                     AVFELGHHPSIITWSAHNEPTGTAPTTAVTPRTGRRAALARIAAQQVPTWNKNVLDRW
                     VKRALEKADESRPVTTHSGVMPHIPEVGGTDSHLWFGWYHGDERDLAGFAVAMPRMVR
                     FVSEFGAQAVPNSTGYIDPAAWPALEWDTLEQRYGLQRAVMERH"
BASE COUNT          496 a         1047 c         1163 g          477 t
ORIGIN      
        1 cgagagccca gccccgcccg gcccgagggc gcggtggatg ccgttgtcgg ccagcgcctc
       61 gaggccgacg gcccgcagcg acgcttcgac cgcggcgtcg ctgggcgcgg agtcgaacag
      121 cgtgatgttg tcgcgcaccg taccggtgaa cagttcgacc tcttgcggaa tcatcgcgac
      181 ccgacgacgt agctcggcga tgggcatctc cgccagcggc accccgccga gcgcgacggt
      241 gccggtggtc ggctcgacca agcgcagcac cagccgcgac nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnttgc
      361 gtcgtcgccg tagtggaagc cgacgtgacg gaactcgatc gacagcggcc ccggcggcgg
      421 catgcgagtg ccggtgtcgc tgatcgaact gcggatcgcg ccgaggtcga tgacgcgcac
      481 aatcgcaccg ttggccttct gcaccgtttc gagctgatcg accatgtcgt tcaagggccg
      541 ggacagcagc gtcatgtact ggaacatcag gaaggccgtg cccagcgaga tgacatcgcg
      601 cgacaccagc actgcgctca acgcgagcgc gacgacgctg cccatcgcga ccaccgcttc
      661 gacggcccac cagacccgga ggaacgcccg ctcccggctg accgcggcgc gcagcgcgtc
      721 ggcactctca ccgacgaagc ggtacatcgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnacca
      901 cgtagacgag ggtgccgacc gcgaggcgcc agtcgagcac cgccagcacg accaccacgc
      961 cgagtaccaa cagcgctgag cccgcggcct tcggtacgac ggagccgagg aagtcggaca
     1021 ccgcggtcac gtcgccgtcg acgcgctgga tcaactcacc gggggtgtgg gtgcggtgga
     1081 actcgtggtc gagcccgagc acgtggcggg tggtgtcgag ccggatcgtg ttggtggtgc
     1141 gccaggcggt gacggtggcg aaccaggcga cgaccacggt caagcattgg gctgcgatcg
     1201 cgacgacgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngagcag cgcgcgccag
     1381 cgggagatcg tcggagcgcc cattcagcca gtttgaccgc catgatggcc aactacggca
     1441 cgaatattcc tcggccgcgg tccggtggaa cagcgccatc catcgccgcg ccctggtcgg
     1501 tacggcgtcg gggtcagcaa cgtcgccgat caggcacgag cgtcgccgtc agatgggggg
     1561 aacgatcacc gtgcggtcgc ggttggtggt ggcaacgttg tcggcttcga gggtgaggtc
     1621 gagccacagg tcgcccggcg cgtcggggac gacgaactgg agtacggcga tgcgggtcac
     1681 ggagtcggcc tcggcggtgc cggtgtacga ccattcgtgt tcgccgttcg accagcgcag
     1741 atgggcgcgc agggtggcgt cggtgaccgg cacgcgcaag tcgttgacga tgtggacgtc
     1801 gaccgcgagc ttgtcgccgg gcgcgagcgc ggcgggcagg cgatcggcca ccacgatcac
     1861 gggacgacag gcctcggtga cggcgaagta gcccatcttc ggttggcgca ggtggtcgag
     1921 cacgctccac gagatggccg ggctggcgtc gttgagcatg aacatcgcca gcccgccggt
     1981 gggtcggtac ttcagccgcc gcagcgtctc gatgtggtgg cgtagcagca ttgcctgata
     2041 gcgctgggtg gcctcgcgcc attgcgcgaa tgtgtcgtag nnnnnnnnnn nnngtgccgt
     2101 tccatgacgg cccgctgcaa cccgtaacgc tgttcgagcg tgtcccactc cagtgccggc
     2161 catgccgctg ggtcgatgta gccggtgctg ttcggcaccg cctgggcgcc gaactcgctg
     2221 acgaagcgga ccatccgcgg catcgccacg gcgaagccgg cgaggtcgcg ctcgtcgccg
     2281 tggtaccaac cgaaccacag gtggctgtcg gtaccaccga cttcggggat gtgcggcatc
     2341 accccgctgt gcgtggtgac cggacggctc tcgtcggcct tctccagtgc ccgcttgacc
     2401 cagcggtcga ggacgttctt gttccaggtg ggtacctgct gcgcggcgat gcgcgcgagc
     2461 gcggcccgtc gtccggtgcg gggtgtgacc gcggtagtcg gcgccgttcc ggtcggctcg
     2521 ttgtgtgccg accaggtgat gatcgagggg tgatggccca actcgaacac ggcctcgtcg
     2581 gcctgggcga ccgcccggcg acggacctcg cgggcgtagc tccactgcag cggaaagtcc
     2641 tgccacagca gcatgcccaa ctcgtcggca gcgtcgtaca gcggctgggc cgagatgtgg
     2701 ccgtggaccc ggagcatgtc gagacccgca tcacgggcca gttcgatgtc acggcggaag
     2761 tcggcgtcgg tcgcgttggc gatgtcgagc cgggtggggg cgaggttggc ccccttgaga
     2821 aacagccgct cgccgttgac gctgaagatc cagtcctgca tcgccacttc gcgcaggccc
     2881 gtgcgagtgc tgcggccgtg gctgaccacg ccgtcggcca acacttcgac gtcgacctgg
     2941 gtcaacggct gatcgccgag cgaccacggc caccacaggg tcgggttggc gacgtcgagg
     3001 ttccagccga ccgcattggc gccgcgggcc aggctctggt cgaattcgga cagcacctcg
     3061 gatcgccgcc ccggaacggt gacggtggtg cggatgcgca cctggcgcgg ttcgtcgctg
     3121 tcgaggcggg cgtgcaggcg caggtgggcg cgggtgtcgt tggcgtcgcg gcacagcacc
     3181 cgcagccggt cgaggcgcac cgggccggtg cgttcgacgg tgaccggtcg ccacagccct
     3241 ccggggttcc acgaccggtc ggcagcgtgc cactgctgga atacaccggt gatgttgcgc
     3301 cgccccttgt gtgagcggtg cggtgcgcac gcgacctcga cggccaggac gtgctcgtcg
     3361 gcgaggcggg ccaggctggt gacgtcgaag gagtggggga agaagtagcc ctcgggatcg
     3421 ccgatgtagg cgccgtcgaa ccacagatcg gcttggtaga acacgccgcc gagcacgacc
     3481 caggcccgtt cgccgtcggc cggcgcgggg aactcgaagc gccgccgcaa cagcaacgga
     3541 ccgtcactgg tggcgaactc gtcgctgtcg cgcca
//
LOCUS       DAORNF010000310         3568 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_19706_length_3568_cov_1.807003, whole genome shotgun sequence.
ACCESSION   DAORNF010000310 DAORNF010000000
VERSION     DAORNF010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3568
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19706_length_3568_cov_1.807003"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..683
                     /locus_tag="PLV68_12420"
     CDS             <1..683
                     /locus_tag="PLV68_12420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02098.1"
                     /translation="SKPKEESDLRTDFWQTVYSLVGGGRFVYKRGMNRLLLLAVLSLV
                     SSPVLRSADPVLKADFASEGLPEGWKGMKGEWKVVDGALVGSELAADQHAAVFNIPDP
                     HVNSSLKMKVRLDGAKGFHLSYNHAKGHLFRVAITGGQATLSMDKDKKDPASKAETLA
                     KETLALKPGEWVEISCTVEGTKVKVTVGDAVLEGTHPNLAKEKTGYRFVVQGQSVAFD
                     EVAFVSGR"
     assembly_gap    948..961
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <962..2012
                     /locus_tag="PLV68_12425"
     CDS             <962..2012
                     /locus_tag="PLV68_12425"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRE02099.1"
                     /translation="TGKPKGALRRSNTGAASAQQGAALLALIGYSPDDVYLPTGPLYH
                     SGPGGFLAVAQMLGQTVVLQRKFNAEDWLRLVDTYKVTSTFSAPTPIRMICALPDEVK
                     AKYDRSSMRKMIANAAPWSFALKQMYLADFPAESLYEVYGSTELGVNCVLLPQDQLRK
                     RGSCGQPAPLVEIKLFDDDGNEVTGTGPDHTGELFVRGPSVFNDYYKQHDKFEADQRH
                     GYQTVGDIAYLDDEGFVYICDRKKDMIISGGMNIYPAEIEAALEAHPDIFESAVFGIP
                     SDEWGETVHAVVVQREGAGLTEGDVVTHAKAHLAGYKVPRSITWSDELPKTGSGKVLK
                     RELRSPFWAGHGSNV"
     gene            2040..2879
                     /gene="era"
                     /locus_tag="PLV68_12430"
     CDS             2040..2879
                     /gene="era"
                     /locus_tag="PLV68_12430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HRE02100.1"
                     /translation="MRSGFITFVGRPNVGKSTLLNAVCGHKVSIVSDKPQTTRKRVRG
                     ILTTDDAQLVFVDTPGLHKPVTALGERVNATALESADDVDVVCLVIDATKPFGSGDQW
                     VADRIDIADAVVVVNKTDAVSKPRLMEQLAKVAALGASAYFPVSARTGEGVAALVAHL
                     AGLMPEGPMFFPDGETSDIPEEEWVAELVREQLLAMFEDELPYSIATRVTEWEWPRIR
                     VEIIVERDSQKGMVIGKKGAVLKQVGQRARAQLPPGVYLELFVKVDKDWQRRPDRVQR
                     LGY"
     gene            complement(2921..>3231)
                     /locus_tag="PLV68_12435"
     CDS             complement(2921..>3231)
                     /locus_tag="PLV68_12435"
                     /inference="COORDINATES: protein motif:HMM:NF022112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HRE02101.1"
                     /translation="RGQVTVELTQQFFEVLSAADPSDPILAVAQIVLTMTRLSGISSV
                     RFTIDGEPVEVPLADASSSEPGQWLFHDDYRQVLDDPVTPATTTTTAPSSSVSSPPGG
                     "
     assembly_gap    3232..3292
                     /estimated_length=61
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3293..>3568)
                     /locus_tag="PLV68_12440"
     CDS             complement(<3293..>3568)
                     /locus_tag="PLV68_12440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02102.1"
                     /translation="PPEKIPDQLTATSTTAATTTTTTMPTTTSSTPDSIAQTTTTTIA
                     ETTTTAQITELVEMYYVVNRQIRLTYVPFERNPPPSQVLLALQQPPST"
BASE COUNT          632 a         1075 c         1208 g          578 t
ORIGIN      
        1 aatccaaacc caaagaagaa tccgatttgc gaacggactt ttggcagacg gtctacagtc
       61 ttgtcggggg cgggcgattc gtatacaaac gcggcatgaa ccgtctcttg ttgcttgccg
      121 tcttgtctct cgtgtcttca cctgtccttc gctccgccga tccggtgctg aaggcggact
      181 ttgccagcga aggtctaccc gagggatgga aagggatgaa gggcgaatgg aaagtcgtcg
      241 acggggcgct ggtcggctcg gagctggcgg cggaccaaca tgcggcggtc ttcaacattc
      301 cggatccgca cgtgaactcg agcttgaaga tgaaggtgcg gctcgatggg gcgaagggat
      361 tccacctcag ctacaatcac gcgaaaggtc acctcttccg tgtcgcgatc acgggcggcc
      421 aggcgacgct atcgatggac aaggacaaga aggatcccgc ctcgaaggcc gagacgctcg
      481 cgaaggaaac cctcgcgctg aagcccggtg aatgggtcga gatctcctgc acggtcgagg
      541 gcacgaaggt gaaggtgacg gtgggggacg ccgtcctcga agggacgcat ccgaatctgg
      601 cgaaggagaa gacgggctat cgtttcgtag tgcagggcca aagcgtggcc ttcgatgagg
      661 tggcgtttgt gagtgggagg tgacgaagag gcggcctaca atcgagcgac gaagaggcgg
      721 cctacgtcac cgaccactgt gacgcgaccg tcgtctatgt cgacgccgag ttcgcgccga
      781 tgttcgatcg catccgcgcc gacattccca aggtcgctca cgtcctggtg ttcgacggcc
      841 gggttccggc gggtatgacc gacctcgacg cgctgatggc ggcggcgagc atcgacgagc
      901 cacagatccc cgaaggcacc gaggccgggg cgacgatgat ctacaccnnn nnnnnnnnnn
      961 ncacaggcaa gcccaagggc gcgctgcggc gcagcaacac cggggccgcg agcgcgcagc
     1021 agggcgctgc gctgttggcc ctcatcggct acagccccga cgacgtgtat ctgcccaccg
     1081 gcccgctcta ccactcgggt cccggcggtt tcttggcggt cgcgcagatg ctgggccaga
     1141 ccgtggtgct gcagcgcaag ttcaacgccg aggactggtt gcgcctggtc gacacctaca
     1201 aggtcacgtc cacgttcagc gcgccgacac cgatccgcat gatctgcgcc ctgcccgacg
     1261 aggtcaaggc caagtacgac cgcagttcga tgcgcaagat gatcgccaac gccgcgccgt
     1321 ggagcttcgc gttgaagcag atgtacctgg ccgactttcc ggccgagtcg ctgtacgagg
     1381 tctacggcag caccgagttg ggcgtcaact gcgtgctgct gccacaggac caactgcgca
     1441 agcgcggctc atgcggccaa ccggcgccgc tggtcgagat caagctgttc gacgacgacg
     1501 gcaacgaggt caccggcacc ggcccggacc acacgggcga gctgttcgtg cgcggcccca
     1561 gcgtgttcaa cgactactac aagcagcacg acaagttcga ggccgaccag aggcacgggt
     1621 accagaccgt gggcgacatc gcctatctcg acgacgaggg cttcgtctac atctgcgacc
     1681 gcaagaagga catgatcatc tccggtggga tgaacatcta cccggccgag atcgaagcgg
     1741 cgctcgaggc gcaccccgac atcttcgagt cggccgtgtt cggcatcccc agcgacgagt
     1801 ggggcgagac ggtgcacgcg gtcgtggtgc agcgcgaggg cgcgggactg accgagggtg
     1861 acgtcgtgac ccatgccaag gcccacctcg ccgggtacaa agtgcctcgc tcgatcacct
     1921 ggagcgacga gttgcccaag accggcagcg gcaaggtgct caagcgcgaa ctgcgcagcc
     1981 cattctgggc cgggcacggc tccaacgtct gagtgacgag gcggcggtag ggtcgccagg
     2041 tgcgttctgg gttcatcaca ttcgtagggc ggccgaacgt cggtaagagc accctgctca
     2101 acgcggtctg cgggcacaag gtcagcatcg tcagcgacaa gccccagacc acccgcaagc
     2161 gtgtccgtgg catcctcacc accgacgacg cccagctcgt gttcgtcgac acccccggcc
     2221 tgcacaagcc ggtcacggcg ttgggtgaac gggtcaacgc gaccgcgctc gaatccgccg
     2281 atgacgtcga cgtggtctgc ctggtcatcg acgccaccaa gccgttcggt tcgggcgacc
     2341 agtgggtggc cgaccggatc gacatcgccg acgcggtcgt cgtggtcaac aagaccgacg
     2401 cggtgtccaa gccacggttg atggagcagc tcgccaaggt tgctgcgctc ggggcgtcgg
     2461 cctatttccc ggtctcggcg cgtaccggcg agggcgtcgc ggcgctggtc gcccacctcg
     2521 ccggtctgat gccggagggg ccgatgttct tccccgatgg tgagaccagc gacatccccg
     2581 aagaggagtg ggtcgcggaa ctggtccgcg agcagttgct ggcgatgttc gaagacgagt
     2641 tgccgtactc gatcgcgacg cgcgtcaccg agtgggagtg gccgcggatc agggtcgaga
     2701 tcatcgtcga gcgtgacagc caaaagggca tggtgatcgg caagaagggc gcggtgctca
     2761 aacaggtcgg ccagcgcgcc cgcgcccaac tgccgcccgg tgtgtatctc gaactgttcg
     2821 tcaaggtcga caaggactgg cagcgccgcc ccgaccgagt gcagcggctc ggctattgac
     2881 ccagcggtgc ggccgggtgc gcatcagccg cccggtggcg tcagccgccc ggtggcgatg
     2941 acaccgacga actcggtgcg gtcgtggtgg tggtcgccgg ggtgaccggg tcgtccaaga
     3001 cctggcggta gtcgtcgtgg aacagccact gccccggctc gctggagctg gcgtcggcaa
     3061 ggggcacctc gaccggttca ccgtcgatgg tgaaccgcac ggaggagata ccgctgaggc
     3121 gggtcatcgt cagaacgatc tgggccacgg ccaagatcgg gtccgacggg tcggccgcgg
     3181 acaagacctc gaagaactgt tgggtcagct cgacggtcac ctgaccccgc tnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnggtgctcg
     3301 gtggttgttg cagcgccaac agcacctggc tcgggggcgg gttcctctcg aacggaacat
     3361 acgtcaggcg aatctggcgg ttgaccacgt agtacatctc gaccaactcg gtgatctgcg
     3421 ccgtggtggt ggtctcggcg atggtcgtgg tggtggtctg ggcgatcgag tccggcgtgc
     3481 tgctggtggt ggtcggcatg gtggtcgtgg tggtcgtcgc cgcagtggtg ctggtcgccg
     3541 tcagctgatc ggggatcttc tccggtgg
//
LOCUS       DAORNF010000311         3564 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_19731_length_3564_cov_2.035338, whole genome shotgun sequence.
ACCESSION   DAORNF010000311 DAORNF010000000
VERSION     DAORNF010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3564
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19731_length_3564_cov_2.035338"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            135..1031
                     /locus_tag="PLV68_12445"
     CDS             135..1031
                     /locus_tag="PLV68_12445"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRE02103.1"
                     /translation="MPTPSASSSPAAPLIDGWLNVFRLTADDPIQAPDAHTAATNELF
                     GGDAASSWLATTLADGITAMDASGVARALIGVTTRAAGAPQPGQGVDFGPVEFGLEAC
                     RREPDRVRLVLQVHDVTLPHAAAAQVRRHGALDEVVAVGVFPAALGCDITDRRLYPLY
                     SACVDLGLPVRINLGIAGPHLASKHQHPAMLEELLIDFPELVVIGCHMGHPYERLIVR
                     LIMKFPNLFLMTSGYLPKYFDPELVRFMGSSRGSGRIVFGSDHPGIPLSRALDEARRL
                     AIGDSARDEFLGAALARLIRWS"
     gene            complement(1072..>1674)
                     /locus_tag="PLV68_12450"
     CDS             complement(1072..>1674)
                     /locus_tag="PLV68_12450"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRE02104.1"
                     /translation="AWGYRGPRSFALPEFDPFWKLVEDSGVLVVLHVSDSGYDRYYDE
                     WSGFDEEMSHFHRLSPFKNTVLFNHRPIEDTVTSLICHGALWRFPGARIALVENGGEW
                     VPPFLEHLDHIYSRAPQEYVERPSDTFKRSIWVQPNHEDDPRELIEAIGADHVLFGSD
                     YPHVEGLADPLDYVRGLDGYSAEDVRKIMGANLMELLRVG"
     assembly_gap    1675..1773
                     /estimated_length=99
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1774..2306)
                     /locus_tag="PLV68_12455"
     CDS             complement(<1774..2306)
                     /locus_tag="PLV68_12455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02105.1"
                     /translation="MTPHPTTSHPITAHPTGRHRPAIDYPVFDADNHFYETRDALTRY
                     LPAHRQGTIEYVEVRGRTKIMVQGRISEFIPNPTFDRIARPGAQEEYFKHGNPDGKTQ
                     RELLGPGIDCPPAFRSAKDRLVHMDEQGLDYALVMPTLISLVEERMRDDPDACADVIH
                     AFNQWMVDEWPFAYEGRV"
     gene            2415..>2599
                     /locus_tag="PLV68_12460"
     CDS             2415..>2599
                     /locus_tag="PLV68_12460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02106.1"
                     /translation="MELTISIPQRLRERAEQHPDRPFAIEAAAPGEVDRGGRSVTYGQ
                     FQHEALRWAGVYRGLGLG"
     assembly_gap    2600..2609
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2610..>2900
                     /locus_tag="PLV68_12465"
     CDS             <2610..>2900
                     /locus_tag="PLV68_12465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02107.1"
                     /translation="LVMMRPSITAYASWIGLSWLRVVEASCNTDFQGAMLTYLINNSR
                     CRVAVVDRQYLPRFVAVAATTPGLETVIVVGGDGAGSDGSVDDQTAGAGFQV"
     assembly_gap    2901..2926
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2927..>3564
                     /locus_tag="PLV68_12470"
     CDS             <2927..>3564
                     /locus_tag="PLV68_12470"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRE02108.1"
                     /translation="AAEPITDLDPPKTWEVAMMIYTSGTTGPSKGVLVPWGHVEETSV
                     GMMPADDLDETDCFYSPFPIFHGSGRAALCLMVRCGGRFVLREQFRATHYWSDIIGFG
                     CTTTGFVGAMTAFLWSQPPSADDATNPLKRAVMMPVVPHFRAFEERFGLRVTSCYATT
                     EIGAPFSTGWDITDPMSCGTVRDGYQVRIVDEHDYEVPVGQAGHLIVRHDRP"
BASE COUNT          539 a         1096 c         1178 g          616 t
ORIGIN      
        1 gctcgttgcg gtcgcgacgt gcggcccgcc gcgagagggc aaccgtgcgc gtcggcgacg
       61 caccatttgc cgaagcgtga ccgccaccga accgtcaccg aaccgtccgg cgagacggac
      121 cgaaaggatc aaccatgccc acaccatccg catcctcgtc gccggccgcg cccttgatcg
      181 atggttggct gaacgtcttt cgcctcactg cggacgaccc gatccaggca cccgacgcac
      241 ataccgcggc caccaacgaa ctgttcggcg gtgacgcggc cagttcgtgg ctggcgacga
      301 cgctggcgga cgggatcacg gcgatggacg cgagcggagt tgctcgcgcg ctgatcggtg
      361 tgaccacccg tgcggccggg gcgccgcagc cgggacaggg cgtcgacttc ggcccggtcg
      421 agttcggtct cgaagcatgt cgtcgtgagc ccgatcgcgt gcggcttgtg ttgcaggtcc
      481 acgacgtgac cctgccccac gcggccgctg cgcaggtgcg tcgacacggc gctctcgacg
      541 aggtcgtcgc ggtcggtgtg ttccccgccg cgctcggctg tgacatcacc gaccggcggc
      601 tctacccgct gtacagcgca tgtgtcgacc tcggtctgcc ggtgcggatc aacctcggta
      661 ttgccgggcc acacctcgcg tcgaagcacc aacacccggc gatgctcgag gagctgctga
      721 tcgacttccc cgaactggtc gtgatcggct gccacatggg tcatccgtac gagcggctga
      781 tcgtccgcct gatcatgaag ttccccaacc tgttcctgat gacctcgggc tacctgccca
      841 agtacttcga tccggagttg gtgcggttca tggggtcgag ccgcggctcg ggccggatcg
      901 tcttcggcag cgatcatccg ggtattccgc tgtcgcgcgc gttggacgag gcgcgtcgac
      961 tggcgatcgg cgacagcgca cgagacgagt ttctcggtgc tgcgctggca cgactgatcc
     1021 gctggagttg accccacccc gtcaactcca gcggacagtc tgcgccgacg ctcaaccgac
     1081 gcggagcaac tccatcaggt tggcgcccat gatcttgcgc acgtcttcgg ccgagtagcc
     1141 gtcgagtcca cgcacgtagt cgagtgggtc ggcgaggcct tccacgtggg gatagtccga
     1201 cccgaacagc acgtggtcgg cgccgatcgc ctcgatcaac tcgcgcgggt cgtcctcgtg
     1261 gttgggctgc acccagatgc tgcgcttgaa ggtgtcggaa ggccgctcga catactcctg
     1321 gggtgcccgc gagtagatgt gatcgaggtg ttccaagaac ggcggcaccc actcgccacc
     1381 gttttccacc aacgcgatcc gcgccccggg gaaccgccac agcgccccgt ggcagatcag
     1441 cgacgtgacc gtgtcctcga tggggcggtg gttgaacagg accgtgttct tgaacggcga
     1501 gaggcgatgg aagtgcgaca tctcctcgtc gaacccgctc cactcgtcgt agtagcggtc
     1561 gtagccggag tcggagacgt gcaacacgac gagcaccccg ctgtcttcga ccagcttcca
     1621 gaacgggtcg aactcgggca aggcgaacga gcgcggcccg cggtagcccc acgcnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnactcggc cctcgtaggc gaagggccat
     1801 tcatcgacca tccactggtt gaaggcgtgg atcacgtcgg cgcatgcgtc agggtcgtcg
     1861 cgcatccgct cttcgaccag gctgatcagc gtcggcatca ccagcgcgta gtcgagcccc
     1921 tgctcgtcca tgtggaccag gcgatccttg gccgagcgaa atgcgggcgg gcagtcgatg
     1981 ccgggaccga gcaactcgcg ctgagtcttg ccgtcggggt tgccgtgctt gaagtactcc
     2041 tcctgtgcgc cgggccgggc gatgcgatcg aaggtggggt tggggatgaa ctcgctgatc
     2101 cggccctgga ccatgatctt ggttcggccg cggacttcga cgtattcgat cgtgccctgg
     2161 cggtgtgcgg gcagataccg cgtcagcgcg tcgcgggtct cgtagaagtg gttgtcggcg
     2221 tcgaacaccg gatagtcgat cgccgggcga tgtcggccgg ttggatgtgc tgtgatcggg
     2281 tgtgatgtcg tcgggtgggg ggtcatgtgg ggctccacgt tctcatcaga agctattgag
     2341 taagcattca ctctcaatta gagtaccgat ggtgccgcac cctgtaaagg tcggaccgaa
     2401 cgaacgaggg gatcatggag ttgacgatca gcattccgca gcggttgcgc gagcgggccg
     2461 agcagcaccc ggatcggccg ttcgcgatcg aggccgccgc cccgggtgaa gtcgatcgtg
     2521 gcggcaggtc ggtgacgtac gggcagtttc agcacgaggc gctgcggtgg gcgggggtgt
     2581 atcgcgggct cggcctgggn nnnnnnnnnc gctggtgatg atgcgcccgt cgatcaccgc
     2641 ctacgcgtcg tggatcggcc tgtcgtggct gcgggtggtc gaggcgtcgt gcaacaccga
     2701 cttccagggc gcgatgttga cgtatctgat caacaactct cggtgccggg tcgcggtggt
     2761 cgatcgccag tacctgccgc gcttcgtcgc cgtcgcggcc accacgcccg gcctggagac
     2821 ggtcatcgtc gtcggcggtg acggggctgg cagcgatggt tcggtcgacg accaaacggc
     2881 tggcgcaggg ttccaggtgc nnnnnnnnnn nnnnnnnnnn nnnnnnccgc agccgagccg
     2941 atcaccgatc tcgacccacc caagacctgg gaggtggcga tgatgatcta cacgtctggt
     3001 accaccgggc cgtcgaaggg tgtgctggtg ccgtggggtc acgtcgagga gacgtcggtc
     3061 ggcatgatgc cagcggatga tctcgacgag accgactgct tctactcgcc gtttccgatc
     3121 ttccacggca gcggtcgcgc cgcactgtgt ctgatggtgc gctgcggtgg gcggttcgtg
     3181 ttgcgcgagc agttccgggc cacgcactac tggagcgaca tcatcgggtt cggctgcacg
     3241 accaccgggt tcgtcggtgc gatgacggcg ttcctgtgga gccagccgcc cagcgccgac
     3301 gacgccacca atccgctcaa gcgggcggtg atgatgccgg tcgtgccgca cttccgagcg
     3361 ttcgaagagc gcttcggtct acgcgtcacg tcgtgttacg cgaccaccga aatcggtgcg
     3421 ccgttcagca cggggtggga catcaccgac cccatgtcgt gcggcaccgt tcgtgacggc
     3481 tatcaggtgc gcatcgtgga cgagcacgac tacgaggtgc cggtcggcca ggccgggcac
     3541 ctcatcgtgc gccacgaccg gccg
//
LOCUS       DAORNF010000312         3564 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_19732_length_3564_cov_1.877173, whole genome shotgun sequence.
ACCESSION   DAORNF010000312 DAORNF010000000
VERSION     DAORNF010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3564
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19732_length_3564_cov_1.877173"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..520
                     /locus_tag="PLV68_12475"
     CDS             <1..520
                     /locus_tag="PLV68_12475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02109.1"
                     /translation="MPIREVRRSLDSGFNAEDPGEPQPFCEQFSVEPPTRSRSSFFYN
                     QSSNSSFSESIFSYPTEQGATAAFEQDRAIASRCNEQTSVFNGLPVTYRIASAARDVS
                     RLSGGAIAVRYTAEDADGRALVTGYAVVRRIGSIVVSANYEVYFRELDNKELDDFLDL
                     TEAAFNRVGETL"
     gene            517..870
                     /locus_tag="PLV68_12480"
     CDS             517..870
                     /locus_tag="PLV68_12480"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HRE02110.1"
                     /translation="MNAGVTPGAGTSASPLVAGSIWLDVDDGGEPSHVVALSGASTIG
                     RDLQCEVVVYDDEASRQHARLRPTPAGWAVEDLGTTNGTSVNAARIVGPALVRVGDVI
                     GVGRARCTVVAPPDK"
     gene            898..>1361
                     /locus_tag="PLV68_12485"
     CDS             898..>1361
                     /locus_tag="PLV68_12485"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HRE02111.1"
                     /translation="MIAGASAAEQHFAALLLCLIVVLGLGRLVGMVFRRIKQPVVIGE
                     ILVGIALGPSLLGVIWPEAQEFLFDTADIPYFKLVASLGLVLFMFIVGMEVDLNVVRR
                     SGKKAAAISLTSIALPFALGFFDRRADPFRFQAFAFALPDLRGRTPIHVGSG"
     assembly_gap    1362..1371
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1372..3063
                     /locus_tag="PLV68_12490"
     CDS             <1372..3063
                     /locus_tag="PLV68_12490"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HRE02112.1"
                     /translation="AAADARACSAPSSDVIDAIADRDQAIASANAAGKQAPTQLVQKE
                     TDFLPFGVFIAVSMCVTAFPVLARILAERNMFKIPLGLLMVACAAIDDIMAFTLLALA
                     TALAGQGSVSDIFVILGKLAVYVVILFVVVRPLIERFVLKPYRRNGNRLQTEQLVLLF
                     IGLMASSYITSKIGVHELIGGFLFGVAVPRNNAPNLFHNIAGKIEGVSVSLLLPVFFV
                     IAGQGVRLQGLGDILPIVAIILVACVGKMAGGIIAARVVGVPKRQSLAVGALMNTRGL
                     TELVVLTIGRDAGVINDKMYTMLVIMAIVTTAMAGPLLNLVYPDRWLLRDIAEAERAR
                     VSSATDRVVVLVRDPEQSLPLAELAAAYGGGRETGSVTLLRFTDPSVGLAGFADDLGA
                     MKTLRETVEAAGLTCQIVSRPAADWAADALVEVARLAPSAVVIEPRDDAVVAAIRATG
                     ADVLIPTKPLAGVGAVAVDGGGSNDGLAAVEIGARIAMHASVPFTASEMTARSAKQLK
                     TLGIGRDDAADGPVVSIGASTDHDVRVLAGERDRLVLTETLSSWTLREPLTMSSI"
     gene            complement(3115..>3564)
                     /locus_tag="PLV68_12495"
     CDS             complement(3115..>3564)
                     /locus_tag="PLV68_12495"
                     /inference="COORDINATES: protein motif:HMM:NF012708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HRE02113.1"
                     /translation="RNHFNDPRARFCAICGIAMHQTSFILTEDVRPPLGVLVMSDGTY
                     HTLITSLVFGRDPDDNVEVREGRAQGVTLVDPGNTISRVHAEIRAVDWDVHIVDHGST
                     NGTFLWSNERQQWDRLATGQSVPLKPGSHVSFGRLTATFESAARQKG"
BASE COUNT          540 a         1151 c         1204 g          659 t
ORIGIN      
        1 gatgccgatc cgcgaggtgc gccggtcgct cgattcgggc ttcaacgccg aggacccggg
       61 tgagccgcag ccgttctgcg agcagttcag cgtcgagcca cccacccgct cgcgctcgtc
      121 gttcttctac aaccagagct ccaactcctc gttctcggag agcatcttca gctacccgac
      181 cgagcagggg gcgaccgccg cattcgaaca agaccgggcg atcgcatcgc gctgcaacga
      241 gcagaccagc gtgttcaacg ggttaccggt cacgtaccgc atcgcgagcg cggctcgcga
      301 cgtctcgcgc ctcagcggtg gcgcgatcgc agtgcgctac acggccgagg atgccgacgg
      361 cagggcgctg gtcaccggct acgccgtggt tcgccgcatc ggctcgatcg tcgtgtcggc
      421 caactacgag gtctacttcc gtgagctcga caacaaggag ctcgacgact tcctcgacct
      481 caccgaggcg gcgttcaatc gggtgggcga gacgctgtga acgccggtgt cacgcccggt
      541 gccggcacga gcgccagtcc attggtcgcc gggtcgatct ggctcgacgt ggacgacggc
      601 ggcgagccgt cgcacgtggt ggcgttgtct ggcgccagca cgatcgggcg cgacctgcag
      661 tgtgaggtgg tggtctacga cgacgaggca tcgcgccagc acgcccggct ccgcccgacg
      721 cctgcgggct gggcggtcga agacctcggc accaccaacg gcacgtcggt caacgcggcg
      781 cgcatcgtcg ggccggcatt ggtgcgcgtc ggtgatgtga tcggcgtcgg gcgggcgcgc
      841 tgcacggtcg tcgccccgcc tgataagtaa acctacagac atgaaacggg ggactccatg
      901 atcgctggtg cgtcggcggc cgaacaacac ttcgccgcac tgttgttgtg tctcatcgtg
      961 gtgctcggcc tgggccggtt ggtcggcatg gtgttccggc ggatcaaaca gccggtggtg
     1021 atcggcgaga tcctggtcgg catcgccctc ggacccagcc tgctcggggt gatctggccc
     1081 gaggcgcaag agttcctgtt cgacacggcc gacatcccct atttcaagct ggtcgcctcg
     1141 ctcggcctcg tgctgttcat gttcatcgtc ggcatggagg tcgacctcaa cgtcgtccgc
     1201 cgatcgggca agaaggctgc ggccatctcg ttgacgtcga tcgcgttgcc gttcgcgctc
     1261 ggcttcttcg atcgccgcgc ggatccgttc cgtttccagg cgttcgcttt cgcgctgccg
     1321 gacctgcgcg gccggactcc gatccatgtc ggcagcgggc annnnnnnnn ngccgcggcc
     1381 gacgcgcggg cgtgctcggc accgtcgagc gatgtgatcg acgcgatcgc ggaccgcgat
     1441 caagcgatcg ccagcgcgaa cgcggcgggc aagcaggcac cgacgcaatt ggtacagaag
     1501 gaaacggact tcctcccgtt cggggtgttc atcgccgtgt cgatgtgtgt gacggcgttt
     1561 cccgtgttgg cccgcatcct ggccgaacgg aacatgttca agatcccatt ggggctgttg
     1621 atggtcgcct gcgcggccat cgacgacatc atggctttca cgttgttggc gttggccacg
     1681 gcactcgcgg gtcaaggcag cgtctccgac atcttcgtca tcctgggcaa gttggcggtc
     1741 tacgtcgtga tcttgttcgt ggtggtgcgg ccgttgatcg agcggttcgt gctcaagccg
     1801 tatcgccgca acggcaaccg tctccagacc gaacaactgg tgctgttgtt catcggcctg
     1861 atggcctcgt cgtacatcac cagcaagatc ggtgtgcacg agttgatcgg tggcttcctg
     1921 ttcggtgtcg ccgtcccgcg caacaacgcg cccaacctgt tccacaacat cgccggcaag
     1981 atcgaagggg tcagcgtctc gctgctgctc cccgtgttct tcgtgatcgc cggacaaggc
     2041 gtgcgcctgc agggcctcgg cgacatcctg ccgatcgtcg cgatcatcct cgtcgcctgt
     2101 gtcggcaaga tggccggtgg catcatcgcc gcccgcgtcg tcggcgttcc gaagcgacaa
     2161 tcgctcgcgg tcggtgcgtt gatgaacacg cgcgggctga ccgagctcgt cgtgctgacc
     2221 atcggccgcg acgccggtgt gatcaacgac aagatgtaca cgatgttggt catcatggcc
     2281 atcgtcacca ccgccatggc gggaccgttg ctgaacctgg tctatcccga ccggtggctg
     2341 cttcgagata tcgccgaagc cgaacgagcg cgggtcagtt cggccaccga tcgcgtcgtc
     2401 gtcttggtgc gcgatcccga gcagtcgttg ccgctggcgg agttggccgc tgcctacggt
     2461 ggaggccgcg aaacgggctc ggtcacgctg ctgcgcttca ccgatccgtc ggtcggcctg
     2521 gccgggttcg ccgacgacct cggcgcgatg aagacgctgc gtgaaaccgt cgaggccgcg
     2581 ggcctgacgt gtcagatcgt ctcccgaccc gccgccgact gggccgccga tgccttggtc
     2641 gaagtcgccc gcctcgcccc gtcggccgtg gtgatcgagc cacgcgacga cgcggtggtc
     2701 gcagcgatcc gtgcgaccgg ggccgacgtc ctgatcccga ccaagccgct cgcgggcgtc
     2761 ggggcggttg ctgtcgatgg tggtggatcc aacgacggtt tggccgcggt cgagatcggg
     2821 gcccgcatcg cgatgcacgc gtcggtgccg ttcaccgcga gtgagatgac cgctcgttcg
     2881 gccaagcagc tcaagaccct cggcatcgga cgtgacgacg cggccgacgg accggtggtc
     2941 agcatcggtg cgtcgaccga tcacgacgta cgcgtgctgg ccggtgaacg cgatcgtctc
     3001 gtgctcaccg agacgctgtc gtcgtggacg ctgcgcgaac cgctgacgat gagttcgatc
     3061 tagcggcagc tcgatcgatt gggcagctcg atctgacggt gccgcggtcg agtgtcagcc
     3121 cttctggcgg gcggctgact cgaaggtggc ggtcaggcgg ccgaacgaca catgcgagcc
     3181 gggcttgagc ggcaccgact ggccagtggc cagacgatcc cactgctgac gctcgttcga
     3241 ccacaggaac gtgccgttgg tcgaaccgtg gtcgacgatg tgcacgtccc aatcgacggc
     3301 gcggatctcg gcgtgcactc gtgagatggt gttgcccgga tcgaccagtg tcaccccttg
     3361 ggcccggcct tcgcgcacct cgacgttgtc gtctgggtcg cgtccgaaca ccaggctggt
     3421 gatcaacgtg tggtaggtgc cgtcgctcat caccaacacg ccgagcggtg ggcggacgtc
     3481 ttcggtgagg atgaagctgg tctgatgcat ggcgatgcca catatcgcgc agaagcgagc
     3541 ccgcggatcg ttgaagtggt tgcg
//
LOCUS       DAORNF010000313         3555 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_19788_length_3555_cov_1.689429, whole genome shotgun sequence.
ACCESSION   DAORNF010000313 DAORNF010000000
VERSION     DAORNF010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3555
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19788_length_3555_cov_1.689429"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="PLV68_12500"
     CDS             complement(<1..307)
                     /locus_tag="PLV68_12500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02114.1"
                     /translation="MTELLTPAAQGATHAPTAGVLLFPGAGTGADHPSLVQIEQSLAP
                     LPVRRADFPYRKAGRRAPDRTPVLLDAVRAEAASFADELGVPPAQVVYGGRSMGGRMC
                     "
     gene            complement(304..>946)
                     /locus_tag="PLV68_12505"
     CDS             complement(304..>946)
                     /locus_tag="PLV68_12505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02115.1"
                     /translation="RRVALGNLVLPVEPAPGFGGLLLGAVVAHHLGDVDDDFHADISR
                     LLDQIDRGERIVQPRLRYRFQVDRHGLATSRHRMEGEGDNVHFSFTTNASALAQVLGA
                     IYAIERLDVAARHALVPVIHKAMRWRGPIGPSFIAHLAGTTSSTLRAMADPRAWALEV
                     FGFPVGTTTLSKKQIVAAYRDRLREVHPDHGGDHGTAHRSIANLGEARRILLT"
     assembly_gap    947..960
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1086..1883)
                     /locus_tag="PLV68_12510"
     CDS             complement(1086..1883)
                     /locus_tag="PLV68_12510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02116.1"
                     /translation="MTDWNTDDDAARVYYDLAEWDFDQQAELTSALAEADIPHEWQGT
                     ELVIPEEHEEITDAIFADVEQRLGILGIGTGTEQRTDVEAVEVRELSDDEPVTDYDLA
                     DWQEAERDLVGDSLAAANIPFRWQDTTLIVPTADEAAVDEILDDVESGEVISAADAGD
                     VDGVPFESLNSFFLAGERLRKDPGDADGLERLVEALDVADPNRPPRGVELRVWRRSCE
                     LADRLADALIAGASDDPDEVEYDEDGLPIVPALQIASDLHDLLRPLI"
     gene            complement(1893..>2124)
                     /locus_tag="PLV68_12515"
     CDS             complement(1893..>2124)
                     /locus_tag="PLV68_12515"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HRE02117.1"
                     /translation="ADRIRTPLLMLHGDADPVVPVEQSRQLAAGIAAAGGNVELHVYA
                     GEGHGLRKPENQLDEYRRIGAFLREYVPGPSR"
     assembly_gap    2125..2282
                     /estimated_length=158
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2283..3537)
                     /locus_tag="PLV68_12520"
     CDS             complement(<2283..3537)
                     /locus_tag="PLV68_12520"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HRE02118.1"
                     /translation="MLYRLGADGNLWLQPVPGGAVRQLTDHGPDRAAQAPMVCPGSDG
                     VVYVIDQREVWLQRFDGTPPRRLDGGDADFCFDPWVTSCGTTVVWQAWDVPDMAWDRS
                     RVQRITFDGTVRDEFQPAGALQQPRTLDDGRAIFVCDETGWNNVWVDDAPLVGEAVEQ
                     ADPTWGMGQRSFAASPDQRHVAFVRNEAGFGRLCVAEIATGAVTEIARGVHGQLSWAG
                     HSLAAIRTGAKTPTQVVVYDMRAFDADAAAAPSTPPPSTPTPSTPPPSMPPARRTIDV
                     GPLSGWEQESLVEPEPLTITARDGAMLHARLYRADAPVRGMICWLHGGPTDQWQVTFM
                     PRIAYWRSQGWHILVPDHRGSTGHGRAYQQAMNGRWGDLDVTDTADVVRHAHTQGWAD
                     PAHTVLMGGSAGGFTVLGVVAAEPDL"
BASE COUNT          524 a         1193 c         1169 g          497 t
ORIGIN      
        1 aacacatccg tccgcccatc gagcgtccgc cgtacacgac ctgcgcgggt ggcacgccca
       61 actcgtcggc gaacgacgct gcttcggcgc gcaccgcgtc gagcagcacc ggggtccggt
      121 cgggggcgcg ccgcccggcc ttgcggtatg ggaagtcggc ccgccgcacc ggcagtgggg
      181 ccagcgactg ttcgatctgc accagcgacg ggtggtcggc accggtgcca gcgccgggaa
      241 acagcaacac ccccgcggtc ggggcgtggg tcgcgccctg cgccgcgggc gtcaagagtt
      301 cggtcatgtc aacaagatcc ttcgtgcttc tccgagattg gcgatcgagc ggtgggccgt
      361 gccgtggtca cccccgtggt caggatgtac ctcgcggagg cggtcgcgat acgcagcaac
      421 gatctgcttc ttcgacaacg tggtcgtgcc gaccgggaag ccgaacacct ccaacgccca
      481 cgcccgcgga tcggccatcg cccgcagggt ggacgaggtg gtcccggcaa ggtgggcgat
      541 gaacgacggc ccgatcggcc cccgccaccg catcgccttg tggatcaccg gcaccagcgc
      601 atggcgcgcc gcgacgtcga ggcgttcgat cgcgtagatc gcccccaaca cttgggccag
      661 cgcgctggcg ttggtggtga agctgaagtg gacgttgtcg ccctcgccct ccatccggtg
      721 ccggctggtg gcgagaccgt ggcgatcgac ctggaagcgg tagcgcaggc ggggctgcac
      781 gatccgctcg ccccggtcga tctggtcgag caggcgcgag atgtcggcgt ggaagtcgtc
      841 gtcgacatcg ccgaggtgat gggcgacgac cgcgcccaac agcaacccac cgaagccggg
      901 cgccggttcg accggcagca ccaaattgcc gagcgcgacc cggcggnnnn nnnnnnnnnn
      961 gccagatctc cagttcggcg agcaacatcg tcaacccgcc tgcggagcga ccatgacgcc
     1021 gacgagacac gccacaccgg cgaggcaggt caagccgaca ccgatcccgg gactgcccaa
     1081 cgagatcaga tcaacggccg gagcagatcg tgcaggtcgg acgcgatctg cagcgcggga
     1141 acgatgggca gaccgtcctc gtcgtattcg acctcgtccg ggtcgtcgga cgcaccggca
     1201 atcagcgcgt cggccaaacg gtcggcgagc tcgcacgaac gtcgccagac gcgcaactcg
     1261 acaccgcgcg gcggacggtt ggggtcggcc acgtcgagcg cttcgaccaa ccgctccagc
     1321 ccatcggcat cacccgggtc tttgcgcaga cgctcaccgg caaggaagaa gctgttgagg
     1381 ctctcgaacg gcacaccgtc gacgtcaccc gcgtcggctg cggagatgac ctcgcccgac
     1441 tcgacgtcgt cgaggatctc gtccaccgca gcctcgtccg cggtcggcac gatcaacgtg
     1501 gtgtcctgcc accggaacgg gatgttggcc gcggcgagcg agtcaccgac cagatcgcgc
     1561 tcggcctctt gccagtcggc caggtcgtag tcggtgaccg gctcgtcgtc ggacaactcg
     1621 cgcacctcga ccgcttcgac gtcggtgcgc tgctcggtcc ccgtgccgat gccgaggatg
     1681 ccgaggcgct gctcgacgtc ggcgaagatc gcgtcggtga tctcctcgtg ctcctccggg
     1741 atgaccaact cggtgccttg ccactcgtgg ggaatgtcgg cttcggccag cgccgaggtc
     1801 agctcggcct gttggtcgaa gtcccactcg gcgaggtcgt aatagacacg cgccgcgtcg
     1861 tcgtcggtgt tccagtcggt cacggagcga ccctatcgtg acggccccgg cacgtactcg
     1921 cgtaagaacg caccgatgcg gcggtactcg tcgagctggt tctccggctt gcgcaggccg
     1981 tggccctcgc cggcatagac gtgcagttcg acgttgccgc ccgcggccgc gatgcccgcc
     2041 gcgagttggc ggctctgctc taccggcacc accgggtcgg catcgccgtg cagcatcagc
     2101 agcggggtgc ggatacggtc ggcgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nncaagatcg ggttcggcgg cgaccacacc gagcacggtg aagcccccag cagagccacc
     2341 catcagcacc gtatgcgccg ggtcggccca cccctgcgtg tgagcgtggc gaacgacgtc
     2401 ggccgtgtcg gtcacgtcga ggtcgcccca ccggccgttc atcgcctgct gatacgcgcg
     2461 gccgtggccg gtggaaccac ggtggtcggg taccaggatg tgccagccct gcgaccgcca
     2521 ataggcgatg cgcggcatga acgtgacctg ccattggtcg gtcggcccac cgtggagcca
     2581 gcagatcatg ccccgcaccg gagcgtcggc ccggtagagc cgggcgtgca gcatggcacc
     2641 gtcgcgggcg gtgatcgtca gtggctcggg ttcgacgagt gattcctgct cccaaccgct
     2701 gagcgggccg acgtcgatcg tgcgccgtgc tggcggcatc gatggcggcg gcgtcgacgg
     2761 tgtgggcgtt gacggtggcg gcgtcgacgg tgcggccgca gcgtccgcat cgaacgcacg
     2821 catgtcgtag acgacgacct gcgtcggcgt cttggccccg gtgcggatcg cggccagcga
     2881 gtggcccgcc cacgacagtt ggccgtgcac accacgggcg atctcggtga ccgcaccggt
     2941 cgcgatctcg gccacgcaca gccgaccgaa cccggcctcg ttgcgcacga acgcgacgtg
     3001 gcgctgatcg ggcgacgcgg cgaagctgcg ctggcccatc ccccacgtcg ggtcggcctg
     3061 ttcgaccgct tcaccgacga gcggcgcgtc gtcgacccac acgttgttcc atccggtctc
     3121 gtcgcagacg aagatcgccc gtccgtcgtc cagagtgcgc ggctgttgca gggcgcccgc
     3181 gggttggaac tcgtcgcgca cggtgccgtc gaacgtgatc cgctgcacgc gtgaccggtc
     3241 ccaggccatg tcgggcacgt cccacgcctg ccacaccacg gtggtgccgc acgacgtcac
     3301 ccacgggtcg aaacagaagt cggcatcgcc gccgtcgagt cgccgcggcg gcgtgccgtc
     3361 gaagcgctgc agccagacct cgcgctggtc gatcacgtag accaccccgt cgctgccagg
     3421 gcacaccatc ggcgcctgcg cggcgcggtc ggggccgtgg tcggtcagtt gccgcaccgc
     3481 cccaccgggc accggctgca gccacaggtt gccgtcggca cccagccgat agagcaagga
     3541 cgacgtgctc aggta
//
LOCUS       DAORNF010000314         3555 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_19789_length_3555_cov_1.385714, whole genome shotgun sequence.
ACCESSION   DAORNF010000314 DAORNF010000000
VERSION     DAORNF010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3555
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19789_length_3555_cov_1.385714"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>209
                     /locus_tag="PLV68_12525"
     CDS             <1..>209
                     /locus_tag="PLV68_12525"
                     /inference="COORDINATES: protein motif:HMM:NF012350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02119.1"
                     /translation="GAGSLDVFVARPVSESTLARLATLVRDAETHASPTQRFTDRFER
                     VFVPAVLALVALVLVIGAIGSGSFA"
     assembly_gap    210..392
                     /estimated_length=183
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <393..1390
                     /locus_tag="PLV68_12530"
     CDS             <393..1390
                     /locus_tag="PLV68_12530"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HRE02120.1"
                     /translation="KTGTLTTGEPRLTDVVPAAGRSDRDLLQVAVAVESASDHPLARA
                     VVRDGAARLGDVPAARATEEASRSAIGAATDVVALTGRGIRAVIDGVIVRAGSAAMFT
                     GQLDAAMAATVGRLQLAGRTTIVVAVGDDVLGVLGVMDTPRTTARAAIESLRSLGMSR
                     AVLLSGDHQAVADAVAGAVGIDEAWGDLLPDDKVAAITRLRDEAGQVAMVGDGVNDAP
                     ALAHATVGIAMGAAGSDVALESADIALMGDDLSALPAIVGLSRRSSRIIRQNLLISLG
                     MVALLVPATLVGAVGIGPAVVAHEGSTLIVVANALRLLAFRPAADRRRSFGGRQR"
     gene            complement(1439..>2384)
                     /locus_tag="PLV68_12535"
     CDS             complement(1439..>2384)
                     /locus_tag="PLV68_12535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HRE02121.1"
                     /translation="WSSVTALDWDTDVDPERLIEWDSPIMTLAREAQTLPGSPISTWG
                     DKEFTALGIESFKAQISQFIHGEQGAMLTAAKIVETVPWIDAKYYAATQTMDEARHTE
                     VFQRYLSEKVGDEYPMSPYLQGQIFGLLEDSRWDIAYLGMQIVIESLALAAFGEMLRR
                     TDEPLLRKLLRYVLADEARHVAFGILSLQEYYEGLSQAELKDRQEFLAENTVRNQLRS
                     TTPEMWERLGVDLHALMPFILEAAGRLKMNPAAKFQRAFFSKLVPNVRKLGLLDANDG
                     YLRTLWADYGLLEFEFAEDTSSDYETYDAVAQDRAAAR"
     assembly_gap    2385..2411
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2742..2773
                     /estimated_length=32
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2774..>3169)
                     /locus_tag="PLV68_12540"
     CDS             complement(<2774..>3169)
                     /locus_tag="PLV68_12540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02122.1"
                     /translation="NDPGISWMFANLCRLLREAVVRFSRVRPARIDGAERVADVLLDF
                     ASTLDTPLSLDEAVRLEAHVTARRQPAVAAAIEEAVAGWRAALDPLIDLAFADGTFAP
                     DIDPDAVAYFMRTVGLGLLLQRASGAKTPD"
     assembly_gap    3170..3179
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          463 a         1046 c         1230 g          564 t
ORIGIN      
        1 ggtgccggga gcctcgacgt gttcgtcgcc cgtcccgtgt ccgaatcgac gctggcgcgt
       61 ctggcgacgc tggtgcgcga cgccgagacg catgcctcgc cgacccagcg gttcaccgat
      121 cgcttcgagc gcgtcttcgt gccggccgtg ctggcgttgg tggcgctggt tctcgtcatc
      181 ggggcgatcg ggtcgggcag ctttgccgan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnacaagacc ggcacgctca ccacgggaga
      421 gccgcgcctg accgacgtcg ttccggccgc cggtcggtcc gaccgtgacc tcctgcaggt
      481 cgccgtcgcc gtcgagtcgg ccagcgatca tccgctggcg cgagcggtgg ttcgcgacgg
      541 tgccgctcgg ctcggcgatg taccggcagc tcgtgcaacc gaagaggcgt ctcgcagcgc
      601 aatcggtgca gcgaccgatg tcgtcgcgct gaccggacgc ggcatccgcg ccgtaatcga
      661 cggtgtgatc gtccgcgccg ggagtgcggc gatgttcacc ggccagctcg acgccgcgat
      721 ggccgcgacc gtcggacggc tgcagctcgc cggccgcacg acgatcgtcg tcgctgtcgg
      781 cgacgacgtg ctcggcgtgc tcggggtgat ggacacgccg cgaaccacgg cacgcgccgc
      841 gatcgagtcg ctgcgctcgc tcgggatgtc gcgcgctgtg ctgctgtccg gcgaccacca
      901 agccgtcgcc gacgcggtcg cgggcgcggt tggcatcgac gaggcgtggg gcgacctgtt
      961 gcccgacgac aaggtcgccg cgatcactcg cttgcgcgac gaggccggcc aggtggcaat
     1021 ggtcggcgac ggggtcaacg acgctccggc actcgcccac gcgaccgtcg ggatcgcgat
     1081 gggcgccgct ggctccgacg tcgcgttgga gagcgccgat atcgccttga tgggcgacga
     1141 cctgtcagct ctgccggcga tcgtcggtct cagccgccga tcgagccgga tcatccgtca
     1201 gaacctgctg atcagtttgg ggatggtggc gctgctcgtg ccggcgacgc tggtgggcgc
     1261 ggtcggcatc ggtccggccg tcgtcgccca cgagggctcc accctgatcg tggtcgccaa
     1321 cgcgctccgt ctgctcgcct ttcgccccgc cgctgatcga cggcggtcat tcggcggccg
     1381 tcaacgctga tccgcgccgt ggtgaggagg gcggcggtga ggagggccgc gggagcagtc
     1441 agcgggcggc ggcgcgatct tgggccactg cgtcgtaggt ctcgtagtcg ctggacgtat
     1501 cttccgcgaa ctcgaactcg agcaggccgt agtcggccca tagcgtgcgc aggtagccgt
     1561 cgttggcgtc gagcaggccg agcttgcgca cgttgggcac gagcttggag aaaaatgccc
     1621 gctggaactt ggccgccggg ttcatcttga ggcggcctgc ggcttcgaga atgaacggca
     1681 tcagcgcgtg caggtcgacg ccgaggcgct cccacatctc tggcgtggtc gagcgcaact
     1741 gattgcgcac ggtgttctcg gccaggaact cctggcggtc cttgagctct gcctggctga
     1801 ggccctcgta gtactcctgc agactgagga tgccgaaggc cacgtggcgg gcctcgtcgg
     1861 cgagcacgta gcggagcagc ttgcgcagca gcggctcgtc ggtgcggcgc agcatctctc
     1921 cgaacgcggc gagcgccagg ctctcgatca cgatctgcat cccgaggtag gcgatgtccc
     1981 accgtgaatc ctcgagcagg ccgaagatct ggccctgcag atacgggctc atcgggtact
     2041 catcgccgac cttctcgctg aggtagcgct ggaacacctc ggtgtggcga gcctcgtcca
     2101 tggtctgggt tgcggcgtag tacttggcgt cgatccaggg caccgtttcg acgatcttgg
     2161 ccgcggtcag catcgcgccc tgttcgccgt ggatgaactg gctgatctgg gccttgaacg
     2221 actcgatacc gagggcggtg aactccttgt caccccatgt ggagatgggg ctgccgggca
     2281 atgtctgggc ctcgcgggcc agggtcatga tcggcgagtc ccactcgatg agacgttcgg
     2341 gatcgacgtc ggtgtcccag tcgagcgcgg tgacgctgga ccacnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn ncttgttgta cagcgtcacc agttgcggcc gctcacgggt gtacgtccac
     2461 aggaaggccc gcggggcgtg gtcgtgcacc cagcggatcg tgtcgtcgac ggtggctgca
     2521 tcgggcagtt cggtgccagg gacgtgtgtc gtggtcatcg cggcggtcat ggcgtgtcct
     2581 cgtgggtcgt gggtcggacg gttgtggtgt gtcggcggtc ggcaacagtc gccggatcga
     2641 tggtgttcgg gctggcagtg gttggatcga cgccggccag accggtggcg gttgggtcgg
     2701 cggtggtggc gatgccagcg aggacgcggc cgatcagcga cnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnngtcgggg gtcttggcgc cggacgcccg ctgcaacagg aggccgagtc
     2821 cgacggtgcg catgaagtag gcgaccgcgt cgggatcgat gtcgggggcg aacgtgccgt
     2881 cggcgaaggc gagatcgatc agcggatcga gtgcggcgcg ccatccggcg acggcctctt
     2941 cgatcgcggc ggcaacggcg ggctggcggc gggcggtgac gtgggcttcc agccgtacgg
     3001 cctcgtccag gctgagcggg gtgtcgagcg tgctggcgaa gtcgagcaac acgtcggcga
     3061 cccgctcggc cccgtcgatg cgagccgggc gcacccgcga aaagcgcacg acggcctcgc
     3121 gcagcaggcg gcacaggttg gcgaacatcc agctgatgcc ggggtcgttn nnnnnnnnnc
     3181 aagcccgact cgcggacgat gtccccgata cgtgtcccct cgtacccctc ccgagcgaac
     3241 acccggcggg cagcatcgag cagcacctcg cgtagttcgt cgtcatcgtc gatcgccacc
     3301 gtgcatccac ccccttcggc cagcacgtgc gccgtgaaga ataaatgttc ttcgccgcct
     3361 gcgcaagtgg taccacgtcg cggggtgtgg ggcgtggggc ggctgtgtcg gcgacgatca
     3421 ggagcggatc gagacggtct tgtcgcggtg gtgatggccg gtgacgatgg cgacttggtt
     3481 ggggcggaca ccgaagtgtt gggcgaccag cgccagcacg gtgagatggc cgagcgaggg
     3541 ggggcagtcg atcag
//
LOCUS       DAORNF010000315         3550 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_19825_length_3550_cov_1.752790, whole genome shotgun sequence.
ACCESSION   DAORNF010000315 DAORNF010000000
VERSION     DAORNF010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3550
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19825_length_3550_cov_1.752790"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..661
                     /locus_tag="PLV68_12545"
     CDS             <1..661
                     /locus_tag="PLV68_12545"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4170 domain-containing protein"
                     /protein_id="HRE02123.1"
                     /translation="RPGGDTLHGAFSESPRGEGRPDLFRRGWHKLKQGPAYRQRCDGH
                     EAAQVGGVVALARGALDQLLAFGNRHMAARGIERRGRRLAVEFVGAFADYESARMAWK
                     AAAQRTVDNGTYASLGEALFNLDMNSGAYSCARCHTTGWSYGDPGESGAGALGPNLTA
                     GATITRFPLESDMIAFVKSGSELGKKYGLQSQGSGKMPGFGSMLTDEQIKLIVEYVRG
                     L"
     gene            661..>1327
                     /locus_tag="PLV68_12550"
     CDS             661..>1327
                     /locus_tag="PLV68_12550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02124.1"
                     /translation="MSSVLLAINWEPQLRGILIIIISTVVLCGSIYVMIGTNIGARLG
                     FLLSLAGLAGWMMLMGMVWWAFGLGLQGQLPSWQPMVGQTILQTPQALAETGVLGDAP
                     ALPGADLDYTAQADALDDLLVANGWTKLAESNPSFGQSASSAGVQIEEDGTLGAGRYQ
                     VVSVFDKGGERYPKIGNSIDFVAFLHKPHYIVVEVATLKEQREEPGRAPARADYAPGC
                     CSRA"
     assembly_gap    1328..1337
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1500..1973
                     /locus_tag="PLV68_12555"
     CDS             1500..1973
                     /locus_tag="PLV68_12555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HRE02125.1"
                     /translation="MGQYLPVIVLMVLAIVFGIASYGASRLLAPRRPSAAKEAPYECG
                     ILPSREPPERFPVSFYIVAMLFVMFDIEIIFLYPYAVSRRDLGPFAFWEMIAFTVVFF
                     ATFVFAVARGALDWGPLQKYRRLDSAVSIERTLTTTIRRVGTEGRLDADEPGQAA"
     assembly_gap    2081..2225
                     /estimated_length=145
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2226..2556
                     /gene="nuoB"
                     /locus_tag="PLV68_12560"
     CDS             <2226..2556
                     /gene="nuoB"
                     /locus_tag="PLV68_12560"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HRE02126.1"
                     /translation="MAPVLRQVYDQMMEPKWVISMGVCASSGGMFNNYAIVQGVDQIV
                     PVDVYAPGCPPTPETLIHAIETLHQHIEDGHIFRRRAETGAGANVHITDIPAMAEPTP
                     VLLGRSS"
     gene            2593..3126
                     /locus_tag="PLV68_12565"
     CDS             2593..3126
                     /locus_tag="PLV68_12565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HRE02127.1"
                     /translation="MDSEAIDAAPAPLQIHGFTVADSLGQQVIHVPRDAYVATVKALL
                     ADGYLQLTDLCAVDYLTHPGRTLPDDVAPERFEVVVNLLDHAHRRRLRIRVQIPAADA
                     TCPTLFDLHPGADAMEREAFDLMGITFTDHPDPSRILMPEDWEGHPLRKDYDAGAIPV
                     QFSHDFALARGERGEPR"
     gene            3123..>3550
                     /locus_tag="PLV68_12570"
     CDS             3123..>3550
                     /locus_tag="PLV68_12570"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439971.1"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HRE02128.1"
                     /translation="MSIIITDDEKAALIGQTSEGEQELRPRHEMTAKELLREAGAVLR
                     LSEKQAAELGAIDTVAVPGQTGDVDEQTMIINMGPQHPSTHGVLRVMLELQGETVLRC
                     KPVVGYLHTGMEKTAEELTYVQGGTNVTRMDYLSPFFNELV"
BASE COUNT          563 a         1183 c         1097 g          552 t
ORIGIN      
        1 gcgcccaggc ggtgacaccc tgcacggtgc tttcagcgaa tccccgcgcg gcgagggcag
       61 gcccgacctt ttccgtcgcg gctggcacaa gctcaagcaa ggccccgctt accggcagcg
      121 ctgcgacggt cacgaagcgg cgcaggtcgg cggcgtggtc gcgcttgcgc gaggcgcgct
      181 cgaccagctg ctggctttcg gcaatcgcca catggcggct cgtggaatcg agcgccgcgg
      241 ccgtcgcctc gcggtcgagt ttgtcggcgc ctttgcagac tatgaaagcg cgcgcatggc
      301 gtggaaggcc gctgcgcagc gcacggtcga caacggcacc tacgccagcc tcggcgaggc
      361 attgttcaac ctcgacatga acagtggcgc ctacagctgc gcccgttgcc acaccacggg
      421 ttggagctac ggcgatcccg gcgagtcggg ggcgggcgcg cttggcccca acctcaccgc
      481 cggtgccacc atcacccgct tcccgctcga gtcggacatg atcgcgttcg tcaagtcggg
      541 ttccgagctc ggcaagaagt acggcctcca gagtcagggc agcggcaaga tgcccggctt
      601 cgggtcgatg ctcaccgacg agcaaatcaa gctcatcgtc gaatacgtgc ggggtctgtg
      661 atgtcgtctg tcttgctcgc catcaactgg gagccgcagc tgcgcggcat cctgatcatc
      721 atcatctcga ccgtcgtgtt gtgtgggtcg atctacgtga tgatcggtac caacatcggt
      781 gctcgcctcg gcttcttgtt gtcgctcgcc ggtctggcgg gctggatgat gctgatgggc
      841 atggtgtggt gggccttcgg cctcggcctc cagggccagt tgccgtcgtg gcagccgatg
      901 gtcggccaga ccatcctgca gacaccgcag gccttggccg agaccggcgt gctcggcgat
      961 gctccagcgc tgccgggtgc cgatctcgac tacaccgccc aggccgacgc gctcgacgac
     1021 ctgctggtcg ccaacggctg gaccaagctg gccgagtcca acccgtcgtt cggccagtcg
     1081 gcgtcgtccg ctggcgtgca gatcgaagag gacggcaccc tcggcgcggg ccgctaccag
     1141 gtcgtgtccg tgttcgacaa gggcggcgag cgctacccca agatcggcaa ctcgatcgac
     1201 ttcgtcgcct tcctgcacaa gccgcactac atcgtggtcg aagtcgccac cttgaaggag
     1261 cagcgtgaag agcccggtcg tgcgccggcg cgggcggatt atgcaccggg gtgttgtagc
     1321 cgagcagnnn nnnnnnnaca tgatccgcga ccttggcaac cagcgcgtgc cggccgcgta
     1381 catcaccatc ggctcggcga ttgtgttctt cgcgacctgt tggttgttgc acagccgcga
     1441 ccgccgggtg atgcagaatc gctccgcggc cctcgccgta ccagccaagg agtgatcgca
     1501 tgggtcagta tctgcccgtc atcgtgctga tggtgttggc gatcgtcttc ggcatcgcca
     1561 gctacggggc ctcgcgcctg cttgcgccgc gtcggccgtc ggcagccaag gaggcgccgt
     1621 acgagtgcgg catcctgccc agccgcgagc caccggagcg gttcccggtc agcttctaca
     1681 tcgtggcgat gctgttcgtg atgttcgaca tcgagatcat cttcctctat ccctacgcgg
     1741 tctcgcgccg tgacctcggc ccgttcgcgt tctgggaaat gatcgccttc acggtggtgt
     1801 tcttcgctac cttcgtgttc gcggtcgccc gtggcgcact cgactggggc ccgctgcaga
     1861 agtaccgccg tctcgactcg gccgtgtcga tcgagcgcac gctcaccacc accatccgcc
     1921 gcgtcggcac cgagggccgc ctcgacgccg acgagccagg tcaggctgcc tgatgggcgc
     1981 gatccagcac atcgtcgacg acggcctcga aggcatccag cacaacgccg tcaccggcaa
     2041 gctcgaagac gtcatcaagt gggcccgcag ccgcagtagc nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnngatgg ccccggtgct gcgccaggtg tacgaccaga tgatggagcc caagtgggtc
     2281 atcagcatgg gcgtgtgcgc cagcagcggc gggatgttca acaactacgc gatcgtgcag
     2341 ggcgtcgacc agatcgtgcc ggtcgacgtc tacgcccccg gttgcccacc cacgcccgag
     2401 acgctgatcc acgccatcga gacgctgcac cagcacatcg aagacgggca catcttccgc
     2461 cgccgggccg agacgggcgc cggcgccaac gtgcacatca ccgacatccc ggccatggcc
     2521 gaaccgaccc cggtgctcct cggccgaagc agttgaccga tgagcgacac cacccccact
     2581 gaggtgtccg ccatggattc cgaagccatc gacgctgccc ctgcacccct gcagatccac
     2641 gggttcaccg tggccgacag cttgggccag caggtcatcc acgtgccgcg cgacgcctac
     2701 gtcgcgaccg tcaaggcgct gctcgccgac ggctatctgc agctcaccga cctgtgcgcg
     2761 gtcgactacc tgacccaccc cgggcgcacg ttgcccgacg atgtcgcgcc cgagcggttc
     2821 gaggtcgtgg tcaacctgct cgaccacgcc caccgccgcc gcttgcgcat ccgggtccag
     2881 atccccgccg ccgacgccac ctgcccgacg ctgttcgacc tgcaccccgg cgccgacgcg
     2941 atggagcgtg aggcgttcga cctgatgggt atcaccttca ccgatcatcc cgatccgagc
     3001 cggatcctga tgcccgaaga ctgggaaggc cacccgttgc gcaaggacta cgacgccggc
     3061 gcgatcccgg tgcagttcag ccacgacttc gccctcgccc gcggagagag gggggaaccc
     3121 cgatgagcat catcatcacc gacgacgaga aggcggcgct gatcggtcag accagcgagg
     3181 gcgagcaaga actgcgcccg cgccacgaga tgaccgccaa ggagttgctg cgcgaggccg
     3241 gcgcggtgct gcggctcagc gagaagcagg ccgccgagct cggcgcgatc gacaccgtgg
     3301 cggtgcccgg ccagaccggt gacgtcgacg agcagacgat gatcatcaac atggggccgc
     3361 agcaccccag cacgcacggc gtgttgcgag tgatgctcga gctccagggc gagaccgtgc
     3421 tgcgctgcaa gccggtggtg ggctacctgc acaccggcat ggagaagacc gccgaggaac
     3481 tgacctacgt gcagggcggc accaacgtca cccgcatgga ctacctcagc ccgttcttca
     3541 acgagctggt
//
LOCUS       DAORNF010000316         3549 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_19835_length_3549_cov_1.769605, whole genome shotgun sequence.
ACCESSION   DAORNF010000316 DAORNF010000000
VERSION     DAORNF010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3549
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19835_length_3549_cov_1.769605"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>245
                     /locus_tag="PLV68_12575"
     CDS             <1..>245
                     /locus_tag="PLV68_12575"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HRE02129.1"
                     /translation="GAIAITLAKRAPGATVWAVDVNQRALRLTRDNARANKVANVQTC
                     TPDQIDPAIRFAGIWSNPPIHVGKAALHDMLSTWLAR"
     assembly_gap    246..363
                     /estimated_length=118
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            462..1145
                     /locus_tag="PLV68_12580"
     CDS             462..1145
                     /locus_tag="PLV68_12580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRE02130.1"
                     /translation="MAGQHLLIVDDEDNLRSMLAAALAHHGFTVSTAADGRAALAAVG
                     TEHPDMILLDVMMPDLDGFEVCRRLRADGDRTPVLFLTARDTTEDKVRGLTLGGDDYL
                     PKPFSLDELVARTEAILRRAGAAATNGTVHECADLVMDDDAHRVHRGSEEVSLSPTEY
                     NLLRYLLINKGRVLSKAQILDHVWKYDFGGDGGVVETYIGYLRRKVDHVEPRLIHTVR
                     GVGYSLRTP"
     gene            1142..>2288
                     /locus_tag="PLV68_12585"
     CDS             1142..>2288
                     /locus_tag="PLV68_12585"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRE02131.1"
                     /translation="MKPSLGRLTLRTRLVAGMGLIALVLVVVVVVITITTHDRLIRQV
                     DARLRSISPASLVPLPTTGADATVTDETPDDVPDLTIGSVPNGGQGNRPFWGRERVSD
                     AYEGWVDADGTLHTRFEPNAGGADYGAPDLSGVELPRRGSLLITTGSSTGAVTYRVLI
                     QPAGPLLSVTAVPLDAVQSTITQLIWVQIGGVGVVLIVLGLVAWWVLRLGIRPIKEMT
                     RTATRIADGDLSVRVPETAPGTESGDLAVALNAMLVHIGTALDERAASEERLRRFVAD
                     ASHELRTPVTTIRHGGLARQDSLDDAMRRTEQEAARMGRLIEDMLTLAKFDEQRPLAT
                     HPVDMITVARDTVADARAAAPERTIELHVDVETAMVAGDADRLHQVLT"
     assembly_gap    2289..2427
                     /estimated_length=139
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2595..>3549)
                     /locus_tag="PLV68_12590"
     CDS             complement(2595..>3549)
                     /locus_tag="PLV68_12590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02132.1"
                     /translation="ALANPGPAGLLRPSIDAERRGWPIVEIIDRTQDEPWQKSMVTSA
                     LIRQLRDHDRTVVAVLNTTGRSRLLACRQCKALQRCERCEASVVQRDDNTFACLRCAT
                     TRPPVCQACGAASFANLRTGVTRLREELEAAAGRPVVAVTATGSDVVPPAGVYVGTEA
                     VLHRVSRADTVVFVDMDAELLAPRYRAAEQAMTLLVRAARLVGGRAGGGRVLVQTFLP
                     AHPVLDAARHADPGRLVPDEMRRRQMLGFPPAAAIALVTGNGAEAYGDSLRASGVESA
                     TTLDGATLLRTNDWATLATACATHPRPAGTRLRIEVDPPRL"
BASE COUNT          497 a         1162 c         1161 g          472 t
ORIGIN      
        1 gggtgcgatc gcgatcacgt tggccaagcg cgctcccggt gccacggtgt gggcggtcga
       61 cgtcaaccag cgcgccctgc gactgacacg tgacaatgcg cgggcgaaca aggtcgccaa
      121 cgtccagacc tgcacgcccg atcagatcga cccggcgatc cgcttcgcgg gcatctggtc
      181 gaacccgccg atccacgtcg gcaaggccgc gctgcacgac atgctgtcga cgtggctggc
      241 ccgctnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnatcggtt cggccaaggg atatcgcgtc ctgagcgcga tgcgcgccga ctctggggtg
      421 actcacagcg aactctgatg ttggcgcgcg acactgctgc catggctgga caacatctgc
      481 tgatcgtcga cgacgaggac aacttgcgct cgatgctcgc ggccgcgctg gcccatcacg
      541 gtttcaccgt gtcgaccgca gccgacgggc gcgccgcgct ggccgccgtc ggcaccgagc
      601 acccggacat gatcctgctc gacgtgatga tgcccgacct cgacgggttc gaggtctgcc
      661 gccggttgcg ggccgacggc gaccgcacgc cggtgttgtt cctcaccgcc cgcgacacaa
      721 ccgaagacaa ggtccgcggc ctcaccctcg gcggcgacga ctacctgccc aagccgttca
      781 gcctcgacga gctcgtggcc cgcaccgaag cgatcctgcg ccgcgccggt gcggccgcga
      841 ccaacggcac cgtccacgag tgcgccgacc tggtgatgga cgacgacgct caccgggtgc
      901 atcgcggcag tgaggaggtg tcgctgtcac ccaccgagta caacctgctg cgctacctgc
      961 tgatcaacaa gggccgcgtg ctgtcgaagg cgcagatcct cgaccacgtc tggaagtacg
     1021 acttcggcgg cgacgggggt gtggtcgaga cctacatcgg ttacctgcgc cgcaaggtcg
     1081 accacgtcga gccgcggctg atccacaccg tccgcggcgt cggttactcg ctgcgcaccc
     1141 cgtgaaaccg tccctggggc gcctgaccct gcggacgcgg ctcgtcgctg gcatggggct
     1201 gatcgccctg gtgttggtgg tcgtggtcgt cgtcatcacg atcaccaccc acgaccgcct
     1261 gatccgtcag gtcgacgcgc gcctgcgttc gatttcaccg gcttcgctgg tgccgctgcc
     1321 gaccaccggc gcggatgcga cggtgaccga cgagacccca gacgacgtgc ccgacctgac
     1381 gatcggcagc gtgcccaacg gcgggcaggg caaccgcccc ttctggggcc gcgagcgggt
     1441 cagcgatgcc tacgagggtt gggtcgatgc cgacggcacg ctgcacaccc gcttcgaacc
     1501 caacgccgga ggcgccgact acggcgcacc cgacctcagc ggtgtcgaac tgccccgtcg
     1561 tggatcgctg ttgatcacca ccggatcgag cacgggcgcg gtcacctatc gcgtcctgat
     1621 ccagcccgct gggccgttgc tcagcgtgac cgcggtgccg ctcgacgcgg tgcagagcac
     1681 gatcacccaa ctgatctggg tgcagatcgg cggcgtcggc gtggtgctga tcgtgctcgg
     1741 cctggtggcg tggtgggtgt tgcgcttggg catccggccg atcaaggaga tgacccggac
     1801 cgcgacccgc atcgccgacg gcgacctcag cgtgcgtgtg cccgagaccg cgccgggcac
     1861 cgagtcgggc gacctcgcgg tcgcgctgaa cgccatgctc gtccacatcg gcaccgcgct
     1921 cgacgaacgg gccgcgtcgg aagaacggtt gcggcggttc gtcgccgatg ccagccacga
     1981 actgcgcaca ccggtcacga ccatccgcca cggtggcctg gcccgccagg acagcctgga
     2041 cgacgcgatg cgccgcaccg agcaggaggc ggcgcggatg ggtcgcctga tcgaggacat
     2101 gctgacgctg gccaagttcg acgagcagcg gccgctcgcg acccacccgg tcgacatgat
     2161 cacggtcgcc cgcgacaccg tggccgacgc ccgggccgcc gcgcccgagc ggacgatcga
     2221 actgcacgtc gatgtcgaga cagcgatggt ggcgggcgat gccgaccggc tgcaccaggt
     2281 gctcaccann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnncag cccgctcgcg ccaccgcggc ggcagcggct
     2461 tgggcctctc gatcgtcgac gccgccatca ccgcccacgg cggcaccctg tcgatcgaca
     2521 cccatcccgg ccaaggcaca acagtccacc tgaccctgcc ccgcacctga ccacgcgctg
     2581 ccgcgtcggc agcgctacag gcgagggggg tcgacttcga tgcggaggcg ggtacctgct
     2641 gggcgggggt gggttgcgca ggcggtggcg agggtggccc agtcgttcgt acggagcagg
     2701 gtggcgccgt cgagggtggt ggcggactcg acgccgctgg cgcgcagtga gtcaccgtac
     2761 gcttcggcgc cgttgccggt gacgagggcg atcgctgccg ccggggggaa gccgagcatc
     2821 tgccggcggc gcatctcgtc ggggaccagg cgaccggggt cggcatggcg ggctgcatcg
     2881 agcaccgggt gcgcgggtag gaacgtctgg accaacaccc gtccgccgcc ggcccggccc
     2941 ccgaccaggc gggccgcgcg gaccagcaag gtcatcgcct gttcggcggc ccggtagcgc
     3001 ggcgcgagca gttcggcgtc catgtcgacg aacaccacgg tgtcggcgcg cgatacccga
     3061 tggagcaccg cttcggtgcc gacgtacacg cctgcggggg gaaccacgtc gctgcctgtg
     3121 gcggtgacgg cgacgaccgg tcgtccggcg gccgcttcca actcttcgcg caggcgggtg
     3181 acgccggtgc gcaggttggc gaagctcgcc gcaccacagg cttggcacac gggagggcgc
     3241 gtcgtggcgc agcggaggca ggcgaaggtg ttgtcgtcgc gctggacgac cgacgcttcg
     3301 caacgctcac agcgttgcaa cgccttgcac tggcggcagg ccagcagccg cgaccggccc
     3361 gtggtgttga gcaccgcgac cacggtgcgg tcgtgatcgc gcaactggcg gatgagcgcg
     3421 gacgtcacca tgctcttctg ccacggctcg tcctgggtgc ggtcgatgat ctcgacgatc
     3481 ggccagcccc tgcgctcggc atcgatgctg gggcgaagca gacccgccgg gcccgggttc
     3541 gcgagtgcg
//
LOCUS       DAORNF010000317         3539 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_19921_length_3539_cov_1.591274, whole genome shotgun sequence.
ACCESSION   DAORNF010000317 DAORNF010000000
VERSION     DAORNF010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3539
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19921_length_3539_cov_1.591274"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>987)
                     /locus_tag="PLV68_12595"
     CDS             complement(<1..>987)
                     /locus_tag="PLV68_12595"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WS/DGAT domain-containing protein"
                     /protein_id="HRE02133.1"
                     /translation="APTDQELLAKTFAELVRRPEKLIRLSVRSMREIAKSTQNGGLRA
                     LADLIAQPIPGPLGVRIRERLRGTNNDVDHPPALPVTQAPRTPFNRPISGHRRFAYTS
                     IPLDDAKRIRRQFGCTFNDVVMALCSGALRRYLLDHDALPDDPLIAMVPVSVRTGDED
                     ETYQNRVSALFADLATNEADPVARLRRVQQSMSAAKEQFAAIPAETLQDFTQFAPPAI
                     AARAMRMYSRLRIADRTNPPFNLVISNVPGPNYPLYSAGAKLEHFYPVSALADGQGLN
                     MTVQSYNGNLDFGFIGCRDLVPDIDRMVDNLHASMAELLACCDTEQASTHPPA"
     assembly_gap    988..1060
                     /estimated_length=73
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1061..>1311)
                     /locus_tag="PLV68_12600"
     CDS             complement(<1061..>1311)
                     /locus_tag="PLV68_12600"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0045017 - glycerolipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HRE02134.1"
                     /translation="WIEDENFDIDFHVRHHAVPAPGTPQQLAEVVSRIVARPLDRTRP
                     LWELYVIEGVDGGTKVAQLTKIHHAAIDGAAGAGMLAAIL"
     assembly_gap    1312..1354
                     /estimated_length=43
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1617..2564
                     /locus_tag="PLV68_12605"
     CDS             1617..2564
                     /locus_tag="PLV68_12605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRE02135.1"
                     /translation="MSRTHIDKADGTLHDVGDGCLAYLQRDGSWGWSNAGLVVGDGAS
                     LLVDTLFDLRLTAEMLAAMKPHVDLAPLSTVINTHANGDHCYGNQLVRDKEIIASARA
                     AHEMTEVPPSMMAALNGAPGQLGELFRHFFGPFQFDGIELTLPTRTFEGRLDVEVGGR
                     TIELIEVGPAHTAGDVIAYVPDAKTVFTGDILFIGGTPIAWAGPLSNWIAACDLMLGM
                     DIETVVPGHGPITDKAGIVAVRDYLAYIDTEATARFHAGMDVWDAARDIALAGFADLG
                     ESGRVAVNVDTVYRSLDPAHKSADVIEQFKRMAVLEQPH"
     gene            complement(2530..3366)
                     /locus_tag="PLV68_12610"
     CDS             complement(2530..3366)
                     /locus_tag="PLV68_12610"
                     /inference="COORDINATES: protein motif:HMM:NF014193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HRE02136.1"
                     /translation="MYRFLLRPKWIVFHLLVLAGIASMLTAANWQWNRYIARNDFVAA
                     VEARQDTARTPPTELTALLDGVHDRPLGTIEYRIATANGHFLPTAQLIQILRVQDGVQ
                     GVNLLWAFQIDDGPIVLVNRGFVPEGVDVGEPTLGQLRIGGTVRVSQQRRLGELTDSS
                     AGVADEVRRIDLTEISERLHLDLAPVYLDFINSDPAPLGELPTPVPAPDVSGGPPHLS
                     YTVQWLLFTAAVVVGWILAVRHSVRQRRRDAARAAAVPGTTADTAAADELSEAAPTPP
                     SA"
BASE COUNT          544 a         1092 c         1227 g          560 t
ORIGIN      
        1 agcgggtggg tgcgtggagg cttgctcggt gtcgcagcag gcgagcagtt cggccatcga
       61 cgcgtgcaga ttgtcgacca tccggtcgat gtcgggcacc agatcgcggc agccgatgaa
      121 cccgaagtcg aggttgccgt tgtagctctg cacggtcatg ttcaggcctt ggccgtcggc
      181 cagcgccgat accgggtaga agtgctcgag cttggcgccc gcgctgtaga gcgggtagtt
      241 cggcccgggg acgttgctga tcaccaggtt gaacggcggg ttcgtccggt cggcgatacg
      301 caggcggctg tacatccgca tcgcccgggc agcaatcgca ggcggcgcga actgggtgaa
      361 gtcctgcagc gtctcggcgg ggatcgccgc gaactgctcc ttcgcggccg acattgattg
      421 ctgcacgcgg cgcaggcgcg cgaccgggtc ggcctcgttg gtggccaggt cggcgaacag
      481 cgccgacacg cggttctggt aggtctcgtc ctcgtcgccc gtgcgaacac tgaccggcac
      541 catcgcgatc agcggatcgt cgggcaacgc gtcgtggtcg agcaagtatc ggcgcagcgc
      601 cccggaacac agagccatca ccacgtcgtt gaacgtgcag ccgaactggc gccggatgcg
      661 cttggcgtcg tcgagcggga tcgaggtgta ggcgaagcgg cggtgtccgg agatcggccg
      721 attgaagggc gtccggggag cctgcgtgac cggcagcgcg ggcgggtgat cgacgtcgtt
      781 gttggtgccg cgcagacgct cgcggatgcg aacgccgagc ggacccggga tcggttgggc
      841 aatgaggtcg gccagcgcgc gcaggccgcc gttctgcgtg gacttggcga tctcgcgcat
      901 gctgcgtacc gagagccgga tcagcttttc gggacggcgt accaactcgg cgaacgtctt
      961 ggcgagcaac tcctgatcgg tcggagcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn aggatcgcgg ccaacatgcc
     1081 ggcaccggcc gcgccgtcga tcgcagcgtg gtggatcttg gtgagctggg ccaccttggt
     1141 gccgccgtcg acaccttcga tcacgtacag ctcccacagc ggacgagtgc gatcgagggg
     1201 gcgagccacg atccggctga ccacctcggc caactgctgt ggggtgcccg gcgcgggcac
     1261 cgcgtggtgg cgcacgtgga agtcgatgtc gaagttctcg tcttcgatcc annnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnaacggc gcgaggaggt gcatccgttc
     1381 ttggagcccc gccttggtcg cctccaaccc ggcaccgccg ggtacaccgg tgatgtcgaa
     1441 gatgttgagc gaggacacgt ggccgaacac ggtcggtgtt tccatgttca agaagctgac
     1501 gtcgattccc ccgagctgac gcatgcgggg cacgttacaa ctcccgtccg atcggcctct
     1561 cacgggacga cgctccggtt cgccctgtcg ggcgccggtt ctgctaccac aggaacatga
     1621 gccgcaccca catcgacaaa gccgacggga ccctgcacga cgtcggcgac ggctgcctcg
     1681 cctatctcca gcgcgacggg agctggggct ggtccaacgc cggcctcgtc gtcggcgacg
     1741 gcgcgtcgct gctggtcgac acgctgttcg atctgcgtct caccgcggag atgctcgcgg
     1801 ccatgaagcc gcacgtcgat ctggccccgt tgtcgacggt gatcaacacc catgccaacg
     1861 gcgaccactg ctatggcaac cagctcgtgc gcgacaagga gatcatcgcc agcgctcggg
     1921 cggcacacga gatgaccgag gtgccgccgt cgatgatggc cgcgctcaac ggcgcgccgg
     1981 gccagttggg tgagctgttc cgccatttct tcgggccgtt ccagttcgat ggcatcgagc
     2041 tgacgttgcc gacgcgcacc ttcgagggcc gcctcgacgt cgaagtcggc ggacgcacga
     2101 tcgagctgat cgaggtcggc ccggcacaca ccgcgggtga cgtgatcgcc tacgtgcccg
     2161 acgcgaagac ggtgttcacc ggtgacatcc tgttcatcgg cggcacgccg atcgcgtggg
     2221 caggaccgct gtccaactgg atcgccgcct gtgacctgat gctgggcatg gacatcgaaa
     2281 cggtcgtgcc cggccacggg ccgatcaccg acaaggctgg catcgtcgcg gtgcgcgact
     2341 acctcgccta tatcgacacc gaggcgacgg cgcgcttcca cgccgggatg gacgtgtggg
     2401 acgcagcccg cgacattgcc ctcgccggct tcgccgatct cggcgagtca ggccgcgtcg
     2461 cggtcaacgt cgacacggtg tatcgctcgc tcgatccggc ccacaagagt gccgatgtga
     2521 tcgagcagtt caagcggatg gcggtgttgg agcagcctca ctgagttcat ccgcggctgc
     2581 tgtgtcggcg gtggtgccgg gaaccgctgc cgcccgcgct gcgtcgcgcc ggcgctggcg
     2641 caccgaatgg cgtacggcca gtatccagcc gacgacgacg gcggccgtga acagcagcca
     2701 ctgcacggtg tacgacaggt gcggtgggcc accggagacg tcgggcgcgg gtaccggcgt
     2761 cgggagctcg cccagcggtg ccgggtcgga gttgatgaag tcgaggtaga ccggcgcaag
     2821 gtcgaggtgc aagcgctcgc tgatctcggt caggtcgatg cgacggacct cgtcggcgac
     2881 gcccgccgag gagtcggtca actcgccgag gcggcgctgc tggctgaccc gcaccgtgcc
     2941 gccgatgcgc aactgaccga gcgttggttc gccgacgtcg acgccctcgg gcacgaagcc
     3001 gcggttgacg agcacgatcg gaccatcgtc gatctggaac gcccacaaca ggttgacgcc
     3061 ctgcacgccg tcttggacgc gcaagatctg gatcagctgc gcggtcggca gaaagtggcc
     3121 gttggcggtg gcgatgcggt actcgatggt gccgagtggc cggtcgtgca ctccatcgag
     3181 gagtgcggtc agttcggtcg gcggggtccg cgccgtgtcc tggcgtgctt cgacagcggc
     3241 cacgaagtcg ttgcgggcga tgtagcggtt ccactgccag ttggctgcgg tcagcatcga
     3301 cgcgatccca gccagcacca gcaggtggaa cacgatccac ttgggacgga gcaggaaccg
     3361 atacatggcc gctccgaccg taatcgcccg gccggtgggc tccaaccccg gcggcggcaa
     3421 tgggcgcggg gtaggttgcc ggttcgtgga cgcagcagag atcacggggg agtggctgtc
     3481 gggtatcttc gccgcgccgg tcagcgtcgg cgagcaacgg cgcatcggcg acggcttgg
//
LOCUS       DAORNF010000318         3534 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_19956_length_3534_cov_1.909744, whole genome shotgun sequence.
ACCESSION   DAORNF010000318 DAORNF010000000
VERSION     DAORNF010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3534
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19956_length_3534_cov_1.909744"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>263)
                     /locus_tag="PLV68_12615"
     CDS             complement(<1..>263)
                     /locus_tag="PLV68_12615"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRE02137.1"
                     /translation="VAGWDPPFEAADILDWDKSVAHLATQAPWWEPGTASGYHGINQG
                     HLVGEVVRRITGRSLGTFFAEEIAGPLGADFHIGLAPEHDHRVS"
     assembly_gap    264..382
                     /estimated_length=119
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<383..617)
                     /locus_tag="PLV68_12620"
     CDS             complement(<383..617)
                     /locus_tag="PLV68_12620"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRE02138.1"
                     /translation="MTVQGSFDPRFAAVADTLAASLASGADRGASVAMVLDGEMVVDV
                     WGGFADAAQTAPWQQDTITNVWSTTKTMMALCAL"
     assembly_gap    648..774
                     /estimated_length=127
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            791..>1510
                     /locus_tag="PLV68_12625"
     CDS             791..>1510
                     /locus_tag="PLV68_12625"
                     /inference="COORDINATES: protein motif:HMM:NF004283.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HRE02139.1"
                     /translation="MADRLTIVEVGPRDGLQNDPVMVPTAVKVELIERLVAAGLDAIE
                     ITSFVRADRIPKLADADEVVRAVHMPPAARIIALAPNVAGLERAIGTLVNEVAVFAAA
                     SDTFNQRNIGRPTAAALDMFRPVAEEALAAGLRVRGYVSTVLGCPYQGDVPLSDVCAV
                     AEALVAMGCHEVSLGDTIGIGNPSGVRAMVAAVADAIGLDRIAVHMHDTYGMGLANTL
                     AAIEAGVRVADTSIGGLGGCPF"
     assembly_gap    1511..1557
                     /estimated_length=47
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1792..1940
                     /estimated_length=149
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1941..>3534)
                     /locus_tag="PLV68_12630"
     CDS             complement(<1941..>3534)
                     /locus_tag="PLV68_12630"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRE02140.1"
                     /translation="LRQFLGGLSRHRPGLLWGGWAAWNLMFLFPMLNGWLLGRGFGAL
                     AAGHTGQVLWYALASFACEAGRMSIIHVAAITWRQAWVHMQTLLRANMLAGQMASGGG
                     EAGTPVRSAGEALTHFRDDVDDATMLVDNMVDTTGGILMVALAGLVLGSTNAPAAAVL
                     LLPLLAVGIATRALFGRIKRYRIADREAAEAVGGFVGDIMSAATTIKVNGATDTVLAR
                     LRMLVDRRRHTAVRDRVLDTGLMSFGDGIVDIGLGLVVIVTAAAIADGRFGVGQLALF
                     TAYLSWLNFVPRVIGRMMARYRQLSVALGRMAPLAAGGAAANLVAQRSLPIGLGETNQ
                     RLPVVRPARVPLVRLDVVGLSATYADGANRSTANSTGNSTASRTVGVHDVSFSLERGS
                     FTVITGPVGSGKTTVLRALLGLAAHANVSGKVLWNGRELDDRAGFLVPPNCAYLPQVP
                     HLISDSVADNIALGPADEATIEYALMLAAVADDIGEMPDGMATMIGPRGLRLSGGQRQ
                     RVAAARALVHQPELVVLDDVSSA"
BASE COUNT          461 a         1093 c         1090 g          448 t
ORIGIN      
        1 gagacccgat ggtcgtgctc gggcgccaac ccgatgtgga agtcggcccc gagcgggccg
       61 gcgatctcct cggcgaagaa cgtgcccaac gagcgaccgg tgatgcgccg caccacttcc
      121 cccaccaggt ggccttggtt gatgccgtgg taaccggacg cggtgcctgg ctcccaccac
      181 ggtgcctgcg tcgccaggtg agcgaccgac ttgtcccagt cgaggatgtc ggctgcctcg
      241 aacggcgggt cccacccggc cacnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnccagcgcg cacaacgcca tcatcgtctt ggtcgtcgac
      421 cacacgttgg tgatcgtgtc ctgctgccac ggcgcggtct gtgctgcgtc ggcgaagccg
      481 ccccacacgt cgacgaccat ctcgccgtcg agcaccatcg ccaccgacgc gccgcggtcg
      541 gcgcccgagg ccaggctggc cgccaacgtg tcggccaccg ccgcgaagcg cggatcgaaa
      601 cttccctgga cggtcatggc gaatcccctc tcgccgccca ccgcgggnnn nnnnnnnnnn
      661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncgatac
      781 cgtggcccgt atggctgatc ggctgacgat tgttgaggtg gggccgcgcg acgggttgca
      841 gaacgacccg gtgatggtgc cgactgcggt caaggtcgag ctgatcgaac ggttggtcgc
      901 ggccggtctg gacgcgatcg agatcacgtc gttcgtgcgc gccgatcgca tccccaagtt
      961 ggccgacgcc gacgaggtgg tgagggcggt gcacatgccg cctgcggcgc gcatcatcgc
     1021 cctcgctccc aacgtcgctg gcctggaacg ggccatcggc acgctggtca acgaggtagc
     1081 ggtgttcgcc gcggcgagcg acaccttcaa ccaacgcaac atcggccgcc cgaccgcggc
     1141 cgcgctcgac atgttccgcc ccgtggcgga ggaggcgctg gccgcaggcc tgcgcgtgcg
     1201 cgggtacgtg tcgaccgtgc tcggctgccc gtaccagggc gacgtcccgc tcagcgacgt
     1261 gtgtgcggtg gccgaggcgc tggtcgcgat gggctgccac gaggtgtcgc tgggcgacac
     1321 gatcggcatc ggtaatccgt ctggcgtgcg ggcgatggtc gccgcggtgg ccgacgcgat
     1381 cggcctcgac cgcatcgcgg tgcacatgca cgacacctac gggatgggcc tggccaacac
     1441 cctggcggcg atcgaggccg gggtgcgtgt ggccgacacg tcgatcggcg ggttgggcgg
     1501 ttgtcccttc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncag
     1561 ggctggacac gggtgtctcc ctcgatgccg tcgtcgcgac cagttggtgg ctggccgacc
     1621 acctcgatcg tgccccctac gcccgcgtcg ccaccgcctt gggtcgcccc gccgcctgac
     1681 ccgcccgatc ccgcggtgaa ttcgacctcc gcggagcgtg gagtggccgg attccggcga
     1741 ctgtgtactc cgcggaagct ggattcgccg ctcgccgctc gccggacggc gnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn cgcgctcgac acgtcgtcga gcaccaccag ttctggttgg
     1981 tggaccagcg cccgcgccgc ggcgacgcgc tgacgttgac cacccgatag gcgcaggccg
     2041 cgcgggccga tcatcgtcgc catcccgtcg ggcatctccc cgatgtcgtc cgcgaccgca
     2101 gccagcatca gcgcatattc gatcgtggct tcgtcggccg gaccgagagc gatgttgtcg
     2161 gccacggagt cggagatcag gtgcggcacc tggggcaggt aggcacagtt cggcggcacg
     2221 aggaaacccg ctcggtcgtc gagctcgcgg ccgttccaca gcaccttgcc gctcacgttg
     2281 gcgtgcgcgg ccaggccgag cagcgcccgc agcaccgtgg tcttgcccga cccgacgggc
     2341 ccggtgatca cggtgaagct cccgcgttcc agcgagaacg acacgtcatg cacgccgacc
     2401 gttcgactgg cggtgctgtt gccggtgctg ttggcggtgc tgcggttggc gccgtcggcg
     2461 taggtggccg acaacccgac gacgtcgaga cgcaccagcg gtacccgcgc cgggcggacc
     2521 acgggcaagc gctggttggt ctcacccagg ccgatgggca aggaccgttg cgcgacgagg
     2581 ttggccgccg cgccgccagc ggccagcggc gccatccgcc ccagcgcgac ggacagttgg
     2641 cgatagcggg ccatcatccg gccgatcacg cggggcacga agttgagcca cgagagatag
     2701 gcggtgaaca gcgcgagttg gccgacgccg aaacggccgt cggcgatcgc cgcggcggtc
     2761 acgatcacga ccaaccccag gccgatgtcg acgatgccgt cgccgaacga catgagaccg
     2821 gtgtcgagca cgcggtcgcg caccgcggtg tggcgacggc gatcgaccag catgcgcagc
     2881 ctggccagca ccgtgtcggt cgcgccgttg accttgatcg tggtggcggc cgacatgatg
     2941 tcacccacga atccgccgac ggcctcggca gcctcgcggt cggcgatgcg gtagcgcttg
     3001 atccgtccga acagcgcccg ggttgcgatg ccgactgcga gcaagggcag caacagcacg
     3061 gccgccgccg gcgcgttggt gctgccgagc accagaccgg cgagggcgac catcaggatg
     3121 ccgcccgtgg tgtcgaccat gttgtcgacc agcatcgtcg cgtcgtcgac gtcgtcgcgg
     3181 aagtgggtga gggcctcgcc cgccgagcgc accggcgtcc ctgcttcgcc gccgccgctg
     3241 gccatctgcc cggcgagcat gttggcccgc aggagcgtct gcatgtgcac ccacgcctgg
     3301 cgccaggtga tcgccgcgac gtggatgatc gacatgcggc ccgcctcgca ggcgaacgac
     3361 gccagggcgt accacagcac ctgaccggta tgtccggccg cgagcgcgcc gaagccgcgg
     3421 ccgagcaacc agccgttgag catggggaac aagaacatca ggttccacgc cgcccatccg
     3481 ccccacagca ggccggggcg atggcggctg aggccaccca agaactggcg cagg
//
LOCUS       DAORNF010000319         3532 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_19976_length_3532_cov_1.784872, whole genome shotgun sequence.
ACCESSION   DAORNF010000319 DAORNF010000000
VERSION     DAORNF010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3532
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19976_length_3532_cov_1.784872"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>352)
                     /locus_tag="PLV68_12635"
     CDS             complement(<1..>352)
                     /locus_tag="PLV68_12635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRE02141.1"
                     /translation="VAAARAAQADGFIALLPQGYDTEIGGQRRLSGGEAQRIAIARAL
                     VADTPVVVLDEATAFADPESERNVQLALANLLAGRTVLVIAHRLHTVVDADQIIVLDG
                     GRIVQRGRHADLVAA"
     assembly_gap    353..439
                     /estimated_length=87
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<440..>1631)
                     /locus_tag="PLV68_12640"
     CDS             complement(<440..>1631)
                     /locus_tag="PLV68_12640"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRE02142.1"
                     /translation="LRIAGILQAILTLASLVPFVLLAEVGRRLLDGQSVDDQWSVVRL
                     ALVVLGVATTLSAALMLWLHLIDARFALDVRRRLLAKLARLPLGWFTERNAAAVQSAV
                     ADDTATLHYYVTHAAVDVIGAIVAPLAVLVYLFVVDAGLAAVLLIPVVAYVFVFASMM
                     QASGSKIGEAQKWAQRMNGEATAYLDGLPVVRVFGGAGASGFRRSVGDYVGFLRGWQG
                     SFVSKKATASLITDPLTFLAIIVVIGGAFLSAGWIDAAGLLPFLLLGTTFGPRLLALA
                     YNAGGLRGARAAAQRIGLTLTEPELDVVTGAAIDATGASADGPGEVRFEGVAFEYRPG
                     VAVLSDIDLHLVPGTVTALVGPSGSGKSTLAALVARFHDPQQGSVRIGGRDVRELAPD
                     DLYRQ"
     assembly_gap    1632..1641
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1667..2635)
                     /locus_tag="PLV68_12645"
     CDS             complement(1667..2635)
                     /locus_tag="PLV68_12645"
                     /inference="COORDINATES: protein motif:HMM:NF016818.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIP domain-containing protein"
                     /protein_id="HRE02143.1"
                     /translation="MSRGFQGTLLKALGAHEHRITVTEVFDVVPGFRRVRCWSPTVFD
                     GFEPKPTDYIRLWVPDPDDPDNEVQRGYTLVDADTAAGTFALDFVLHDPMGPASLWAS
                     TCQPGDTLPATVYGSHGFEPRVPEPAGYLLIGDAASIPAINSMLAVIPAHVAIEVRLE
                     WSDEREQSVPVAAHPNATVVWLRRDLTGEAVLASLAGRDFADWVAWVGAERGTVKAIK
                     EALREQFGFPKTDLKATAYWIHGRSFGTSRKGSVRAEAKASGEAYRAELAQGTTGESG
                     PDAPDGATATATATATATATATDSAAAARVSEPAAPPVNVERRWRS"
     gene            2731..>3097
                     /locus_tag="PLV68_12650"
     CDS             2731..>3097
                     /locus_tag="PLV68_12650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02144.1"
                     /translation="MSEVAASTSRLTPVQQQTLAALRRSSEPLVFDEAFIADLRDEAL
                     EALAHFTDRIGNRELFVTKHTLAGVFTCETQFLLPDDFSWTPARARGQVAHRAIQLLL
                     NWRGDPEPIELVDSAITRLI"
     assembly_gap    3098..3315
                     /estimated_length=218
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3316..>3532
                     /locus_tag="PLV68_12655"
     CDS             <3316..>3532
                     /locus_tag="PLV68_12655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02145.1"
                     /translation="EGRESRKLIVDLKTGRVHSRHREDLRFYALVETLSRQVPPRKLA
                     SFYLDAGEPVVEDVTEALLRSTLRRTLD"
BASE COUNT          550 a         1194 c         1019 g          454 t
ORIGIN      
        1 cggccgccac cagatcggcg tgacgacctc gttgcacgat gcgaccgccg tcgagcacga
       61 tgatctggtc ggcgtcgacg acggtgtgca gacggtgagc gatcaccaag accgtgcgcc
      121 ccgccaacag gttggcgagc gcgagttgga cgttgcgctc ggactcgggg tcggcgaacg
      181 cggtggcttc gtcgaggacc acgaccggtg tatccgcgac caacgcccgg gcgatcgcga
      241 tccgctgggc ctcgccgccg gacagacggc gctgcccgcc gatctcggtg tcgtagccct
      301 gtggcaacaa cgcgatgaac ccatcggcct gagccgcccg agcggccgcg acnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnc ctggcggtac aggtcgtccg gcgccaactc gcgcacgtcg
      481 cgcccaccga tgcgcacgct gccctgctgc gggtcgtgaa agcgcgccac cagcgcggcg
      541 agcgtcgact tgcccgaccc cgacgggccg accagcgcgg tcacggtgcc cggcacgaga
      601 tgcagatcga tgtcgctcaa caccgcgacg ccgggccggt actcgaacgc gacaccctcg
      661 aagcgcacct cacccggccc gtccgcgctc gcaccagtcg catcgatcgc agcacccgtc
      721 acgacgtcga gctccggttc ggtcaacgtc agaccgatcc gctgcgcggc ggcccgcgcg
      781 ccgcgcagcc cacccgcgtt gtacgccagc gccaacagcc gcggaccgaa cgtggtgcca
      841 agcagcagga acggcaacag cccggcagcg tcgatccacc cggccgacag gaacgcaccg
      901 ccgatcacca cgatgatcgc caggaacgtc aacggatcgg tgatcagcga ggcggtcgcc
      961 ttcttcgaca cgaacgagcc ttgccaaccg cggaggaagc cgacgtagtc gccgacgctg
     1021 cgccggaacc cgctcgcccc cgctccgccg aacacccgca ccaccggcag cccgtcgaga
     1081 taggccgtcg cctcgccgtt catccgctgg gcccacttct gcgcctcgcc gatcttgctg
     1141 ccgctggcct gcatcatcga tgcgaacacg aacacatagg ccaccaccgg gatcagcaac
     1201 acggccgcca accccgcgtc gaccacgaac aggtacacca acaccgccag cggcgcgacg
     1261 atcgcgccga tcacatcgac cgccgcatgg gtgacgtagt agtgcaacgt cgccgtgtcg
     1321 tcggcaacag cgctctgcac cgcggccgcg ttgcgctcgg tgaaccaccc cagcggcaag
     1381 cgggccagtt tggccagcag acggcgccgc acgtcgagcg cgaagcgagc gtcgatcagg
     1441 tgtagccaga gcatcagcgc cgcgctcagc gtcgtggcga ccccgagtac gaccaacgcg
     1501 aggcgcacca ccgaccactg gtcgtcgacc gactgcccgt cgagcagacg ccgccccacc
     1561 tcggccagca gcacgaacgg caccagcgag gccaaggtca ggatcgcctg gaggatgccc
     1621 gcgatccgca annnnnnnnn nccgaagcca acagttcggc acccgcctac gaccgccacc
     1681 gccgttcgac attcaccggc ggcgcggccg gctcgctgac ccgcgccgcg gccgcagagt
     1741 cggttgctgt tgctgttgcg gttgctgttg ctgttgcggt tgctgtcgct ccatccggcg
     1801 cgtctggccc agactcgccg gtggtgccct gggcgagttc ggcacggtac gcctcaccgc
     1861 tggccttcgc ttcggcccgc accgaaccct tgcggctcgt gccgaagctc cgcccgtgaa
     1921 tccagtacgc cgtcgccttc agatcggtct tggggaagcc gaactgctcg cgcagcgcct
     1981 ccttgatcgc cttgaccgtg cctcgttcgg caccgaccca ggccacccag tcggcgaagt
     2041 cgcgcccggc cagtgaggcc aacaccgcct cgccggtcag gtcgcggcgc agccagacca
     2101 ccgtggcatt ggggtgtgct gcaaccggta ccgactgctc gcgttcgtcg ctccactcga
     2161 gacggacctc gatcgcgaca tgcgcgggaa tcaccgcgag catcgaattg atcgctggaa
     2221 tcgatgcagc atcaccgatc aacagatagc cggcgggttc gggaacgcgc ggttcgaacc
     2281 cgtgcgagcc gtacacggtg gccggcaacg tgtctcccgg ctgacacgtc gaggcccaca
     2341 gcgacgccgg tcccatcggg tcgtggagca cgaagtcgag cgcgaacgtg cccgccgcgg
     2401 tgtccgcgtc gaccagggtg tagccgcgct gcacctcgtt gtcggggtcg tcggggtctg
     2461 gcacccacag gcggatgtag tcggtcggtt tgggctcgaa cccgtcgaac acggtcggcg
     2521 accaacagcg cacgcgccga aagcccggta ccacgtcgaa gacctcggtc accgtgatcc
     2581 ggtgctcgtg tgctccgagc gccttcaaca aggtgccctg aaagccacgg ctcatcgggt
     2641 cacgtccgca ttgccgacca gaccatgtaa ggcgttcttc acgtcctgca gggtacatgt
     2701 ggcgcgcacc ccttcgttaa cgtcgggcac gtgagcgagg tcgctgcatc cacgtcgcgc
     2761 ctgacgccgg tccaacagca gacgctggcc gcgctgcgcc gcagcagcga gccgctggtg
     2821 ttcgacgagg cgttcatcgc cgatctgcgc gacgaggcac tcgaagcact cgcccacttc
     2881 accgaccgga tcggcaaccg cgaactcttc gtcaccaagc acaccctggc cggggtgttc
     2941 acctgcgaga cgcagttcct gctacccgac gacttcagct ggacacccgc ccgggcgcgc
     3001 gggcaggtcg cccaccgggc tatccaactc ctgctcaact ggcgcggcga tcccgaaccg
     3061 atcgagctcg tcgacagtgc gatcacccga ctgatcgnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnngaggg acgggagagc cgcaagctga tcgtcgacct caagaccggc
     3361 cgcgtgcaca gccgccatcg tgaagacctg cgcttctatg cgctcgtcga gacgttgtcg
     3421 cgccaggtcc caccgcgcaa gctggccagc ttctacctcg acgccggtga gccagtggtc
     3481 gaagacgtca ccgaggcgct gctgcggtcg acgctgcgcc gcacgctcga cg
//
LOCUS       DAORNF010000320         3532 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_19977_length_3532_cov_1.761576, whole genome shotgun sequence.
ACCESSION   DAORNF010000320 DAORNF010000000
VERSION     DAORNF010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3532
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19977_length_3532_cov_1.761576"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>210
                     /locus_tag="PLV68_12660"
     CDS             <1..>210
                     /locus_tag="PLV68_12660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02146.1"
                     /translation="RSGGSSRSSGGSRSANTGSRQFASRGAGRTGGVTRPGEVTRKPE
                     KTLGGEQVEGRQAVRELLLAGARKV"
     assembly_gap    211..274
                     /estimated_length=64
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <275..>572
                     /locus_tag="PLV68_12665"
     CDS             <275..>572
                     /locus_tag="PLV68_12665"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HRE02147.1"
                     /translation="HERVQVLEVNRRKLEAAARSEAPQGVIAFAAPLPEYELADFLKR
                     RAGKAPFLVAVDGVTDPGNLGALLRCCDGAGVTGVVLPRHRAVHITPTAAKSAAG"
     assembly_gap    573..582
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <583..>917
                     /locus_tag="PLV68_12670"
     CDS             <583..>917
                     /locus_tag="PLV68_12670"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HRE02148.1"
                     /translation="VGLEMRRTMQKHAGVFRFDGLLKEGVAKMKEVGIWIVGLDDAAD
                     RSLFELGDLGAEGICLVLGAEGAGLSRLVRERCDLIVSIPMRGRLSSLNVSATAALAT
                     YEVARARQA"
     assembly_gap    918..1031
                     /estimated_length=114
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1032..2272
                     /locus_tag="PLV68_12675"
     CDS             <1032..2272
                     /locus_tag="PLV68_12675"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HRE02149.1"
                     /translation="EGREIVGLDAGLDDDLADLLDGLRAFLRAEVMPRHEADPALADP
                     RTMFGADGRFRDDVLHQIRAVRRAAADAGYYTMLAPPELGGGGVGFEGLFRAWELIYE
                     ECGPHHFLGYWALAHWARGPSRVLLELDAAEREQVLPDLLSGEHSMCFAMSEPDAGSD
                     AWAMRTTATRAGDGWVINGTKQWITNGPYAEHTLVFAVTDADAARAHRGGISAFLIDA
                     DAPGYRVDSVIAMFGHAGGDEAILSFDDVFVPDRRRVGALGDGMRIAMSGVSSGRLYN
                     TARSVGLARWALAQAFGHTEQRQAFGRKIIEFQGISHRLATAAIELRAARLLGLDCAK
                     RLDAGASGRSDLAAAKVFATEMAARAIDTAVQAHGAMGFTNEMGLAEAWQQVRRIQVA
                     DGSAEVLRNQVVKSIRAGEW"
     gene            2278..3393
                     /locus_tag="PLV68_12680"
     CDS             2278..3393
                     /locus_tag="PLV68_12680"
                     /inference="COORDINATES: protein motif:HMM:NF014790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRE02150.1"
                     /translation="MRSLVLDPDEAAFADEVAAFFAGRAGGRGFFSGRGGATRELYRE
                     LGAKGWLSLTWPVAAGGSGHSPVYDYLVWDAAAYSRAARPDLGPGIVAHMIVHHGTDA
                     QRAAYLPGIAAGKTCFALGYSEPEAGSDLTGLRTRAVRDGDHYVVSGEKCWTSDAHHG
                     DYLWLLCRTGSRESRARGLTLLIVSLGTPGVTVTPIETIDGHRLNQVFLDDVAVPVDQ
                     RVGEEGAAWGLIREALAIERHLQLLPGRVRADLDDFGRLMDDLAIAARPDVRAATQEL
                     TARYLAVEAASLHTLAALCAGQPGVVEAATTKLLGTRLAQDIPRRALDLAGPAGLDRA
                     SMPAFLWRQSIMETIAGGTSEVMLSILGREALGLGAR"
BASE COUNT          475 a         1166 c         1196 g          507 t
ORIGIN      
        1 gtcggagcgg cggcagtagc cgtagcagtg gtggcagccg ctcggccaac accgggtcgc
       61 gccagttcgc ttcccgcggc gcaggccgca ccggcggggt gacccggccg ggcgaagtga
      121 cccgcaagcc cgaaaagaca ttgggcggcg agcaggtcga aggtcgccag gccgtgcgcg
      181 aactgctgct agcaggcgcc cgcaaggtcc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntcacga acgggtacaa gtgctggagg
      301 tcaaccgccg caagctggaa gcagcagcac gcagcgaagc gccccagggc gtgatcgcgt
      361 ttgcggcgcc gttgcccgag tacgagttgg ccgacttcct caagcggcgc gcgggcaagg
      421 caccgttcct ggtcgcggtc gacggggtga ccgacccggg caacctcggc gcgctgttgc
      481 gctgctgcga cggcgcaggt gtgaccggcg tggtgctgcc tcgccaccgg gcggtgcaca
      541 tcacccccac cgcagccaag agtgcggcgg gcnnnnnnnn nnatgtcggc ctcgagatgc
      601 gccgcacgat gcagaagcat gctggcgtgt tccgcttcga cggccttttg aaggaaggtg
      661 tggcgaagat gaaggaggtc ggcatctgga tcgtcggact cgacgacgcc gccgatcgca
      721 gcctgttcga gctcggcgat ctgggtgccg agggcatctg cctggtgctc ggtgccgaag
      781 gtgccggctt gtcacgcctg gtacgcgaac gctgcgatct gatcgtcagc atccccatgc
      841 gcggtcgcct gtcgtcgctc aacgtctcgg ccacggccgc cctcgccacc tacgaagtcg
      901 cccgcgcccg ccaggccnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1021 nnnnnnnnnn nctgagggcc gcgaaatcgt cggtctcgat gcgggtctcg acgatgacct
     1081 cgccgacctg ctcgacggct tgcgcgcctt cctgcgcgcc gaggtgatgc cgcggcacga
     1141 ggccgacccg gcgctggccg acccgcggac catgttcggt gccgacggca ggttccgtga
     1201 cgacgtcctg caccagattc gtgcggtgcg ccgcgctgca gcggacgccg gctattacac
     1261 gatgctcgct ccccccgaac tgggtggcgg tggggtcggg ttcgagggcc tgttccgcgc
     1321 ctgggagctg atctacgaag agtgcggccc gcaccacttc ctcggctact gggccctcgc
     1381 ccactgggcc cgcggcccga gtcgcgtgct gctcgagctc gacgccgccg aacgcgagca
     1441 ggtgctgccg gacctgttga gcggtgagca ctcgatgtgc ttcgcgatgt ccgaacccga
     1501 cgcaggctcc gacgcctggg cgatgcggac cacggcgacc cgggcgggcg atggctgggt
     1561 gatcaacggc acgaagcagt ggatcaccaa cgggccgtat gccgagcaca ccctggtctt
     1621 cgccgtgacc gacgcggacg cggctcgtgc ccatcggggc ggcatcagtg ccttcttgat
     1681 cgatgccgac gcgcccggct accgcgtgga cagcgtcatc gcgatgttcg gccacgccgg
     1741 tggtgacgag gcgatcctca gcttcgacga cgtgttcgtc cccgaccgtc gccgcgtcgg
     1801 cgcgcttggc gatgggatgc gcattgcgat gtcgggggtg tcgagcggac gcctgtacaa
     1861 caccgctcgc tcggtcggcc tggcccgttg ggcgctggcg caggcgttcg ggcacaccga
     1921 gcagcgccag gcgttcggcc gcaagatcat cgagttccag ggcatctccc accgtttggc
     1981 gaccgccgcg atcgagctgc gcgccgcccg actgctgggc ctcgactgcg ccaagcgtct
     2041 cgacgcgggc gcctctggcc gcagcgacct cgccgcggcc aaggtgttcg ccacggagat
     2101 ggcggcacgc gcgatcgaca cggccgttca ggcgcacggt gcgatgggct tcaccaacga
     2161 gatgggcctg gccgaggcgt ggcaacaggt gcgccggatc caggtcgccg acggctcggc
     2221 cgaggtgctg cgcaaccagg tcgtcaagag catccgcgcc ggggagtggt gacggcgatg
     2281 cggagcctcg tgctcgatcc ggacgaggcg gcattcgccg acgaggtcgc ggcattcttc
     2341 gccggccggg caggcgggcg ggggttcttc tcgggtcgtg gcggcgcgac gcgcgagctg
     2401 taccgcgagc tgggcgccaa gggatggctg agcctcacct ggccggtcgc cgccggtggt
     2461 tccggccact cccccgtgta cgactatttg gtctgggacg ccgcggccta ctcgcgggcc
     2521 gcccggcccg atctcggtcc cggcatcgtc gcccacatga tcgtccacca cggcaccgac
     2581 gctcagcgcg cggcgtacct gccgggcatc gccgccggca agacctgctt cgcgctcggc
     2641 tactccgaac ccgaagccgg cagcgatctc accggcctgc gcacccgcgc ggtgcgcgac
     2701 ggcgatcact acgtcgtcag cggtgagaag tgctggacct ccgacgctca ccacggcgac
     2761 tacctgtggc tgctctgccg cacgggttcg cgcgagtcgc gcgcccgcgg attgaccctg
     2821 ctgatcgtct ccctcggcac acccggtgtc acggtgaccc cgatcgagac gatcgatggc
     2881 catcgcctga accaggtctt cctggacgac gtcgcggtcc ccgtcgatca acgggtcggc
     2941 gaggagggcg ccgcgtgggg gttgatccgc gaggcactcg ccatcgaacg ccatctccaa
     3001 ctgctgcccg ggcgagtgcg cgccgacctc gacgacttcg ggcgcctgat ggacgacctc
     3061 gccatcgccg cgcggcccga cgtgcgcgcc gccacacagg agctgaccgc gcgctacctc
     3121 gcggtcgagg cagcctcgtt gcacacgctg gccgcgctgt gcgccgggca accgggcgtg
     3181 gtcgaagcgg cgaccacgaa gttgctcggc acgcggctcg cccaggacat cccccggcga
     3241 gcgctcgatc tcgccggacc ggcgggactc gaccgggcgt cgatgccagc gttcctgtgg
     3301 cgtcagtcga tcatggagac catcgcgggc ggcacgagcg aagtgatgct gtcgatcctg
     3361 ggccgcgagg cgttggggct gggcgcacga tgagaccgct gttcgaaccc tccgccgatc
     3421 aggttgagct cgaacgggcg atcgcgaccg catgcgggcg actcctggtg ggtgacgcga
     3481 cgtccacctg gcgcacgtcg tggcagacac tcgccggtct gggcatcgtc gg
//
LOCUS       DAORNF010000321         3532 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_19978_length_3532_cov_1.616336, whole genome shotgun sequence.
ACCESSION   DAORNF010000321 DAORNF010000000
VERSION     DAORNF010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3532
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19978_length_3532_cov_1.616336"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            389..1021
                     /locus_tag="PLV68_12685"
     CDS             389..1021
                     /locus_tag="PLV68_12685"
                     /inference="COORDINATES: protein motif:HMM:NF025580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4328 domain-containing protein"
                     /protein_id="HRE02151.1"
                     /translation="MGAGPTRSTRGLGMATIVLFWVTTAIGGLLMFMLINRKTVVNDF
                     FDDASFGSISKIDDADNAVAGALTFLYVGIIASVILSCVWSIRAAGNAKARRPDLNVS
                     PGLAGGSWFIPLGNYVVPFMQLRRAAAAIGGSLTALVFWQVGFIAWNVSFGVIASSGS
                     AINDAKSLDDALGAIDRQVVFGVLMFVAMIVASIFAMQSIRKFDQLGEAR"
     assembly_gap    1146..1320
                     /estimated_length=175
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1321..1725
                     /locus_tag="PLV68_12690"
     CDS             <1321..1725
                     /locus_tag="PLV68_12690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02152.1"
                     /translation="AVCVAAWSMRLALSAQARGIEGISTGWAIAGWFVPIGFLFMGFR
                     QVTKVVAGAGGYVGRVYSWQLAFAATAVVMTVAQTTSSDIAAVATDAAVSALTREAAL
                     STASTVLFAAAALFAGRAITHADAQVSALPLA"
     gene            complement(1746..2330)
                     /gene="leuD"
                     /locus_tag="PLV68_12695"
     CDS             complement(1746..2330)
                     /gene="leuD"
                     /locus_tag="PLV68_12695"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HRE02153.1"
                     /translation="MKAVQIITGTAVPLKRSDVDTDQIIPAEWLKRVERTGFEKGLFS
                     TWRDDRDFVLNDERFAGATILVAGPSFGVGSSREHAVWAIQQYGFDAVIAPSFSDIFR
                     NNCTKNGLVPVVQPLDAVTRIWDAIDADPNVSITIDMQRLQVEIPAIGYEESFPMEPQ
                     NQHRFLNGLDDVGITLTHADDIAAYESRRPAWLR"
     gene            complement(2334..>2568)
                     /locus_tag="PLV68_12700"
     CDS             complement(2334..>2568)
                     /locus_tag="PLV68_12700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HRE02154.1"
                     /translation="RAAGADFREPGCSMCLAMNPDKLAPGERSASTSNRNFEGRQGRG
                     GRTHLVSPAIAAATAVAGHFATPADLSPISTGA"
     assembly_gap    2569..2584
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2585..3150)
                     /locus_tag="PLV68_12705"
     CDS             complement(<2585..3150)
                     /locus_tag="PLV68_12705"
                     /inference="COORDINATES: protein motif:HMM:NF012550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HRE02155.1"
                     /translation="MIEYRGSAIRSLSMEGRMTVCNMSIEAGAKAGLIAPDETTFEYL
                     AGRPNAPQGADWDAAVADWKTLRTDDGAVWDREVFIDASTLTPHVSWGTNPAQVLPIG
                     GSVPSPDAFDDPTARETVARALDYMGLQPGTAIRDIPVDTVFIGSCTNGRIEDLRAAA
                     AVLDGRTVTAKRVMIVPGSHAVKAQAER"
     assembly_gap    3172..3325
                     /estimated_length=154
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3326..>3532)
                     /locus_tag="PLV68_12710"
     CDS             complement(<3326..>3532)
                     /locus_tag="PLV68_12710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HRE02156.1"
                     /translation="IADPISARQVEVLRENTAEFGVQLFPMGDPLQGIVHIIAPEQGR
                     TLPGMTVVCGDSHTATHGAFGALA"
BASE COUNT          436 a         1024 c         1155 g          572 t
ORIGIN      
        1 cggggatgtg ttccgtggcg ggcttgcttg gccggcgccg acgagtgcga ccagcaccga
       61 gacgggctgg ttgatctggc atgtcggttg accagggtac ccagacgatg ccccagagca
      121 gcggattcgc cgaaaccaga tgactcggca tgacaggggt gggcaagatc gtcgcgtcgc
      181 gttgtggtga cgtgccagca tgtacgccat gtcgtcgaca ccgcctccac cgcccggttt
      241 cccgcagcct cctggtcagc ctccgttcgg gtcgcagccg gctccgttcg gccagcctgg
      301 ccctgtcccc aaccaacccg gttacggtca gccccagttc ggtcaacccg caccgcctcc
      361 cgggtaccag gccttcggcg gcgcaccgat gggcgcagga ccgacccgct cgacgcgcgg
      421 gttgggcatg gccaccatcg tgctgttctg ggtcaccacc gccatcggcg gcttgttgat
      481 gttcatgctg atcaaccgca agacggtcgt caacgacttt ttcgacgacg ccagctttgg
      541 atcgatctcc aagatcgacg acgccgacaa tgcggtggct ggtgccttga ccttcttgta
      601 cgtcggcatc atcgcgtcgg tcatcctctc ctgcgtctgg tcgatccggg cggccggcaa
      661 cgccaaggcg cggcgccccg atctcaacgt cagccctggc ttggcgggtg gtagttggtt
      721 catcccgctc ggcaactacg tggtgccgtt catgcaactg cgccgggctg ctgcggccat
      781 cggcgggtcg ttgacggcgt tggtgttctg gcaggtcggg ttcatcgcct ggaacgtctc
      841 gttcggtgtg atcgcgtcgt caggcagcgc gatcaacgat gccaagagcc tcgacgacgc
      901 gctcggcgcg atcgaccgcc aggtggtctt cggtgtgttg atgttcgtgg cgatgatcgt
      961 tgcctcgatc ttcgcgatgc agtcgatccg caagttcgac cagctcggcg aggctcgctg
     1021 agaccggttg gccgatgcgg cgccccgagc tgtacaccta gcctggcgtc gtggactact
     1081 acgcgccgac ctatgagacg aacgagccgg ccagcccgat cccggcgctg agtcggctcg
     1141 tcgtcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 gctgtgtgcg tggcggcttg gtcgatgcgg ctcgcgctca gcgcgcaggc gcgcggcatc
     1381 gaaggcatca gtaccggctg ggcgatcgcg gggtggttcg tcccgatcgg gttcttgttc
     1441 atgggtttcc gccaggtcac caaggtggtc gccggtgccg ggggatatgt cgggagggtc
     1501 tattcgtggc agctcgcctt cgccgcgacg gccgtcgtga tgaccgtcgc ccagaccacg
     1561 tcgtccgaca tcgccgccgt ggccaccgat gccgcagtgt cggcgctgac ccgcgaagcc
     1621 gcgttgagca cggcctccac ggtgttgttc gcggcggcag cgttgttcgc cggacgggcg
     1681 atcacccacg ccgacgccca ggtcagcgcg ctaccgctgg cgtaggccgc gccgcgcgcc
     1741 ggagctcagc ggagccaggc ggggcggcgg gactcgtagg cggcgatgtc gtcggcgtgg
     1801 gtcagggtga tgcccacgtc gtcgagtccg ttgaggaatc ggtgctggtt ctgcggctcc
     1861 atcgggaacg actcctcgta gccgatggcg gggatctcca cctgcaggcg ttgcatgtcg
     1921 atcgtgatcg acacgttcgg gtcggcgtcg atcgcgtccc agatgcgggt gaccgcgtcg
     1981 agcggctgca ccaccggcac cagtccgttc ttggtgcagt tgttgcggaa gatgtcgctg
     2041 aagctgggcg cgatgacggc atcgaagccg tattgctgga tggcccacac ggcgtgctcg
     2101 cgtgacgacc cgacgccgaa gctcggcccg gcgaccagga tggtcgcgcc cgcgaagcgc
     2161 tcgtcgttga ggacgaagtc gcggtcgtcg cgccacgtcg agaacaggcc cttctcgaac
     2221 ccggtgcgct cgacccgctt cagccattcg gcggggatga tctggtcggt gtcgacatcg
     2281 ctgcgcttga gcgggacggc ggtaccggtg atgatctgga cggctttcat cgctcaggct
     2341 cctgtggaga tgggggacag atcggccggg gtggcgaagt ggccggcgac ggcggtggcg
     2401 gcggcgatcg ccggggagac gaggtgggtg cgcccgccgc ggccctggcg gccttcgaag
     2461 ttgcggttgc tggtgctggc cgaacgctcg ccaggagcga gcttgtcggg gttcatcgcc
     2521 aggcacatgc tgcaccctgg ctcgcggaag tcggcgcccg ccgcgcggnn nnnnnnnnnn
     2581 nnnntcccgc tcggcctggg ccttcacggc atggctgccg gggacgatca tgacccgctt
     2641 ggcggtgacg gtgcggccgt cgagcacggc ggctgcagcg cgcaggtctt cgatgcggcc
     2701 gttggtgcaa ctgccgatga acacggtgtc gaccgggatg tcgcggatgg ccgtgccggg
     2761 ctgcaggccc atgtagtcga gggcgcgggc gaccgtctcg cgggcagtcg gatcgtcgaa
     2821 ggcgtcgggc gaaggcaccg atccgccgat cggcagcacc tgagccgggt tggtgcccca
     2881 gctgacgtgg ggcgtcaacg tcgaggcgtc gatgaacacc tcgcggtccc acaccgcacc
     2941 gtcgtcggtg cgcagcgtct tccagtcggc gacggcggcg tcccagtcgg cgccttgggg
     3001 tgcgttgggg cgaccggcga ggtactcgaa cgtggtctcg tcgggggcga tcaggcccgc
     3061 cttggccccg gcttcgatgc tcatgttgca gacggtcatg cggccttcca tgctgagcga
     3121 gcggatggcc gagccccggt actcgatcac gtggccgatc ccgccgccgg tnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnaacgc cagcgcgccg aacgcgccgt gggtggcggt
     3361 gtggctgtcg ccgcagacca ccgtcatgcc cggcagggtg cggccctgct cgggggcgat
     3421 gatgtggacg atgccctgga ggggatcgcc catgggaaag agctgcacac cgaactcggc
     3481 ggtgttctcg cgcagtacct cgacctggcg agccgagatc gggtcggcga tc
//
LOCUS       DAORNF010000322         3530 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_19990_length_3530_cov_1.799424, whole genome shotgun sequence.
ACCESSION   DAORNF010000322 DAORNF010000000
VERSION     DAORNF010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3530
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19990_length_3530_cov_1.799424"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="PLV68_12715"
     CDS             <1..592
                     /locus_tag="PLV68_12715"
                     /inference="COORDINATES: protein motif:HMM:NF012708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HRE02157.1"
                     /translation="AGPTPSSIAGDHDGRTISKAQLAAMRAGANLDTAAAAPSPATPS
                     LGGPSVQAVLCPNRHGNPTHLAVCRECGLALSGPPVLIARPPLGVLVLSTGERVTLDR
                     PAILGRNPKLEGRVPSELPQVIKLEAGANLSRSHAMVRLEGWQVLIEDLNSQNGTVVV
                     TPGQSPRRLRAGEPALLEPGTVVDLGGEVTLTYQVR"
     gene            799..>1510
                     /locus_tag="PLV68_12720"
     CDS             799..>1510
                     /locus_tag="PLV68_12720"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HRE02158.1"
                     /translation="MSRRQPKPPPMLDGFTAGRLIGVGGYADVYLYEQHRPAREVAVK
                     VLAVDVLHDDSQRRRFTTEADLMARVSRHPYIVPVFQADIARDGRPYIVMEYYPGANF
                     LERVRREQFSVADVLRVGINIASAVETAHRAGILHRDLKPANILTSEFGRPGLTDFGI
                     AAPDDQQNDEADGLSIPWAPPEAFGADRLDERADVYSLAATVYHLLAARSPFEISGVS
                     STPLELIARIERLPVPPIG"
     assembly_gap    1511..1569
                     /estimated_length=59
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1570..2343
                     /locus_tag="PLV68_12725"
     CDS             <1570..2343
                     /locus_tag="PLV68_12725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02159.1"
                     /translation="DHRPRTAGEFGRLLQSIEAEMHLAVTPLDLAGESLAAARPIEVN
                     PTDDSTRIRGVVSISPDAPAPTAPAPVAISGVPSAPGPVAPRPERERQGMLGVPSLDR
                     TVHHPPPMALGGAEPGAAPRRARRGVMIAAALLAVVAAVVAIVVLSGDDGEDADARTA
                     VPTESTGFSGTIVVAVAPDPVVDLSGSADGDGRFTYTWSAPADAPVGIQYQVSELVGN
                     EWKFRPPQTDTTFTGDGSCVEVVSIVAGQISAPTKECVS"
     gene            2340..>3530
                     /locus_tag="PLV68_12730"
     CDS             2340..>3530
                     /locus_tag="PLV68_12730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02160.1"
                     /translation="MKRWLTRLTASRTRTVANLSVLGVASAAFIVVATNADGYKATNI
                     DVNDGSVWVSDTGRGRVGRLNVVIDEMDFDVTTSPGPDVLQVGRSIVAVDSGGAATID
                     PITGGYAQPTNGIPVGAYQVGGTTGLVFDAATGNLWTGRASSIVAPEYPDGAHGRVAP
                     GAKVVVSASGRGYVVEPDAGRWFEVLLDNQSRPIISAPVLGTDVTGTVPTTDDAATVD
                     PPATDTSVPEPGPLIVPTAFTALTNPVSVATVVTTVGEQLVFLDPDGSVYGPTGAIAH
                     VPGGDWAIQPPGTDTAPRPGTVLVASSEGLFEITIGSDSVDQIAHNTGHPAQPVRVGR
                     CAYGAWSAEVSSYYTSCQALENVSLNGVGPGDTLVWRVNQGNVALNSTGNGGVWAVHD
                     GRLSE"
BASE COUNT          518 a         1212 c         1208 g          533 t
ORIGIN      
        1 ggccgggccg acgccgagtt cgatcgcggg cgaccacgac gggcggacca tcagcaaggc
       61 ccaactggcg gcgatgcgcg ctggcgccaa tctggatacc gcggccgccg caccgtcacc
      121 ggccacaccg tcgctcggcg gcccgagcgt gcaggcggtg ttgtgcccga accggcacgg
      181 caaccccacc catcttgcgg tgtgccgtga gtgcggcctg gcgctgtccg gcccgccggt
      241 gctgatcgct cggccgccgc tgggcgtgct ggtgttgagc accggcgagc gggtgaccct
      301 cgatcgtccg gcgatcctgg gccgcaaccc gaagctggag ggtcgcgtcc cgagcgaact
      361 gccgcaggtg atcaagctgg aagcgggcgc caacctgtcg cgcagccacg cgatggtgcg
      421 cctggaaggg tggcaggtgc tgatcgagga cctcaactcg cagaacggca cggtggtggt
      481 caccccaggc cagtcaccgc gccgactccg tgcgggcgag cccgcgctgt tggagccggg
      541 cacagtggtc gatctgggcg gcgaggtcac gttgacctac caggtgcggt gatcccaggg
      601 tgggtattgc ggcggctctg acgatgctcg atgcgtcccg gcgtagctcg cccatcgggc
      661 gcgcacaaca cgatgtgttc ctaggtgtga cgatgcctcg ttggacgcag tacttttccc
      721 ggcgggacac gacagaactt ggtgggcgtt gacgctgatg accgcgggca gcacagggac
      781 gaacagggag gctgtgcggt gagccgtcgc cagccgaagc caccgccgat gctcgacggc
      841 ttcaccgcgg gccgtctgat cggtgtcggc ggctatgccg acgtatacct gtacgagcag
      901 catcgcccgg cgcgcgaggt cgcggtcaag gtgcttgcgg tcgacgtgtt gcacgacgac
      961 tcccagcgac gccgcttcac gaccgaggcc gatctgatgg cgcgggtgtc gcgccacccg
     1021 tacatcgtcc ccgtgttcca ggccgacatc gcgagggacg gtcggccgta catcgtgatg
     1081 gagtactacc ccggggccaa ctttctcgag cgggtccggc gcgagcagtt ctccgtggcc
     1141 gacgtgttgc gggtcggcat caacatcgcc agcgcggtcg agacagcgca tcgcgccggc
     1201 atcctgcacc gcgacctcaa accggccaac atcctcacca gcgagttcgg ccgtcctggc
     1261 ctgaccgact tcggcatcgc cgcgccggac gatcaacaga acgacgaggc cgacggcttg
     1321 tcgattccgt gggcaccacc cgaagcgttc ggcgccgacc gcctcgacga acgggccgac
     1381 gtctactcgc tggccgccac cgtctaccac ctgctcgcgg cgcgttcgcc gttcgagatc
     1441 agcggcgtgt cgagcacccc gttggagttg atcgcccgca tcgagcgcct gccggtgccg
     1501 ccgatcgggc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnng atcaccggcc gcgcaccgcg ggcgagttcg gccggctgct gcagagcatc
     1621 gaagccgaga tgcacttggc cgtcacaccg ctcgacctgg ccggcgagtc gttggctgcg
     1681 gctcgcccga tcgaggtcaa ccccaccgac gactccaccc gcatccgcgg cgtggtgtcg
     1741 atctcacccg acgcacccgc ccccaccgcg ccggcgcccg tcgcgatcag cggagtgccg
     1801 agcgcgcctg gcccggttgc gccccggccc gagcgcgaac gccaaggcat gctcggcgtg
     1861 cccagccttg accggaccgt gcaccatcca ccaccgatgg cattgggtgg ggccgaacct
     1921 ggcgccgcgc cgcgccgtgc gcgccgtggc gtcatgatcg cggctgcgtt gttggcggtc
     1981 gtcgccgcgg tggtcgcgat cgtcgtgctg agcggcgacg acggcgaaga tgccgacgcg
     2041 cgcacggctg tgccgaccga atcgaccggc ttctccggca cgatcgtggt ggcggtcgct
     2101 ccggaccccg tcgtcgacct gtccggatcg gccgacggtg acggtcggtt cacctacacc
     2161 tggtcggctc cggccgatgc ccccgtgggc atccagtacc aagtgtcgga actggtcggc
     2221 aacgagtgga agttcaggcc accgcagacc gataccacct tcacgggtga cggttcctgc
     2281 gtcgaggtcg tgtcgatcgt ggccggtcag atttcggcgc cgaccaagga gtgcgtgtcg
     2341 tgaagcggtg gctcactcgt ctgactgcga gccgcacccg caccgtggcc aacctcagcg
     2401 tgctgggtgt cgcttcagcg gccttcatcg tcgtggccac caacgccgac ggttacaagg
     2461 ccaccaacat cgacgtcaac gacggctcgg tctgggtcag cgacaccggc agaggccggg
     2521 tcgggcggct caacgtggtg atcgacgaga tggacttcga tgtgacgacc tcgccggggc
     2581 cggacgtgtt gcaggtgggc cgatcgatcg tcgccgtcga cagcggcggt gcggccacga
     2641 tcgacccgat cacgggtggc tacgcgcagc ccacgaacgg catcccggtc ggcgcttacc
     2701 aggtcggtgg cacgacgggg ctcgtgttcg atgcggcgac cggaaacctc tggaccgggc
     2761 gggcgtcgtc gatcgtcgcg cccgagtatc ccgacggtgc acacggtcgc gtcgctcctg
     2821 gcgccaaggt ggtcgtcagc gcgtcggggc gggggtacgt ggtcgaaccc gatgccgggc
     2881 gctggttcga agtgctgctc gacaaccagt cgcgcccgat catctccgcg ccggtactcg
     2941 gcaccgacgt caccggcacg gttcccacca ccgacgacgc ggcgacggtc gacccgccgg
     3001 caactgacac atccgtgccc gagccgggtc cgttgatcgt gcccaccgcg ttcaccgcgc
     3061 tgaccaaccc ggtgtcggtc gcgacggtgg tcaccaccgt cggcgagcag ttggtgttcc
     3121 tcgatcccga cggttcggtg tacggcccca ccggcgcgat cgcgcacgtg ccgggcggcg
     3181 actgggcgat tcagccgccc ggcaccgaca ctgccccgag accaggaacc gtgctggtcg
     3241 cgagctccga aggtttgttc gagatcacca tcgggtccga ctcggtcgac cagatcgccc
     3301 acaacacggg ccacccggcc cagccggtgc gggtcggcag atgtgcctac ggcgcgtggt
     3361 cggccgaggt gtcgtcgtac tacacctcgt gccaggcgct ggagaacgtg tcgctcaacg
     3421 gcgtcggccc cggtgacacc ctggtgtggc gcgtcaacca gggcaacgtc gccctcaaca
     3481 gcaccggcaa tggcggtgtc tgggcggtgc acgacgggcg tctcagcgag
//
LOCUS       DAORNF010000323         3511 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_20147_length_3511_cov_1.786748, whole genome shotgun sequence.
ACCESSION   DAORNF010000323 DAORNF010000000
VERSION     DAORNF010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3511
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20147_length_3511_cov_1.786748"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>365)
                     /locus_tag="PLV68_12735"
     CDS             complement(<1..>365)
                     /locus_tag="PLV68_12735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02161.1"
                     /translation="ANDVDAVGDRFQVHMDREALNDYPLGKYDVTIEIITYEPDREIA
                     WLVRGSLDLGHSYGYRLEPTVVSTDDGDVEGTTVTSYYDWSAVAPEWKAGVFPVLPES
                     SLRATLGILARTISHRPGR"
     assembly_gap    366..375
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(560..883)
                     /locus_tag="PLV68_12740"
     CDS             complement(560..883)
                     /locus_tag="PLV68_12740"
                     /inference="COORDINATES: protein motif:HMM:NF022169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2510 domain-containing protein"
                     /protein_id="HRE02162.1"
                     /translation="MTDEGLPSDEMIGEQMPAQWRPDPYGRAQQRWWDGDRWTERVAT
                     GGEESTDPLGATATIPFAAPFNPSAPAERSRLRRIITSLGPKRIAAVTLAAAAIVVAL
                     VLTLT"
     gene            1054..>2048
                     /locus_tag="PLV68_12745"
     CDS             1054..>2048
                     /locus_tag="PLV68_12745"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HRE02163.1"
                     /translation="MPYLPGLDGMRALAVVAVMIFHANHAWMPAGFLGVEVFFVISGY
                     LITLLLIAEDERDGGVSLRAFWGRRFRRLLPALYLTLIGLVIYCALFMREQLGHLRGD
                     VVAAVFYVSNWFQIWVGQGYGSLTAFVPLRHLWSLAVEEQFYLVWPLVMVWLLRKGRE
                     RLPQVGLQLIAAAFVIAAVTSILFHKGVVSGVESTPDAYWSLFGRDIDTNNALYLSTI
                     TRASGILLGAGFAMLWRPVAMMRGPMRTKGRQLDLIAAVALVLLVGQMLTYELIGERT
                     ARYFPPLFNGGLLLTGVLTVVLIAAVTHSETLISKLLGHPVLNWIGTRSYGLYLF"
     assembly_gap    2049..2058
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2059..>2467
                     /locus_tag="PLV68_12750"
     CDS             <2059..>2467
                     /locus_tag="PLV68_12750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02164.1"
                     /translation="IIRKQAGLALSLPKFVVALVLTGLVTEASYRFLEMPVRRREFFQ
                     RLRTPTPQVLMGMAAVALLTGYGGVSLLTADLRCTTKIECDAEAARDALAQSTTTTQT
                     GNTQTSTTGTPNSDATGNSDATTTSTTAAVRQIP"
     assembly_gap    2468..2541
                     /estimated_length=74
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2542..2965
                     /locus_tag="PLV68_12755"
     CDS             <2542..2965
                     /locus_tag="PLV68_12755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02165.1"
                     /translation="DAAESRGPNSVLTQVQAYAAPSNQVDVMVIQVGTNGAVTQAQYD
                     AIAAAAAPVGKVFFMTIKTPYDYTEPNNAIIRALPNTYPNVQIIDWQTEAAKVEDGLS
                     KADGGIHLNSGTAVRFYANLILTAAGLEPIPDPDSGGG"
     gene            3041..>3201
                     /locus_tag="PLV68_12760"
     CDS             3041..>3201
                     /locus_tag="PLV68_12760"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF012286.2"
                     /note="catalyzes the oxidation of malate to oxaloacetate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HRE02166.1"
                     /translation="MTSPLRVAVSGAAGQIGYSLLFRIASGEMLGKDQPVILQLLDLP
                     QAQKAVKGV"
     assembly_gap    3202..3211
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3212..>3511
                     /locus_tag="PLV68_12765"
     CDS             <3212..>3511
                     /locus_tag="PLV68_12765"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440290.1"
                     /note="catalyzes the oxidation of malate to oxaloacetate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HRE02167.1"
                     /translation="LDDCAFPLLADTVCTDSADVAFGDADVVLLVGAMPRKAGMERSD
                     LLSANGGIFKPQGEALSRSAKKDVKILVVGNPANTNALIAMHNAKDLDPGRFTAMT"
BASE COUNT          533 a         1135 c         1153 g          586 t
ORIGIN      
        1 ccgccccggg cggtgggaga tggtgcgggc gaggatgccg agggttgctc ggagggagga
       61 ttctgggagg acggggaaga cccctgcttt ccactcgggg gccacggcgg accagtcgta
      121 gtacgacgtc acggtcgtgc cttcgacgtc gccgtcgtcg gtgctgacaa cggtgggttc
      181 caggcggtag ccgtaggagt ggccgaggtc gagactgccg cgcacgagcc aggcgatctc
      241 ccggtcgggc tcgtaggtga tgatctcgat cgtcacgtcg tacttgccga gcgggtagtc
      301 gttgagcgcc tcacgatcca tgtggacctg gaagcgatcg ccgaccgcgt cgacgtcgtt
      361 cgcccnnnnn nnnnnagaac cggtgctcga ctggagcatg ccggacgagt cgatctcgac
      421 gtggccctcc ggatcgcgca atacggcgaa aatctccgcc gccggatgcg ggatgaaccg
      481 cgccacctcg aaccgctcga ccacctcaga tgcgctcatc tccgggatgt tacggtgccc
      541 gtcggcgaac gaagccgcgt caggtgaggg tgaggacgag cgcgacgacg attgcggcgg
      601 cagcgagggt gaccgctgcg atcctcttgg ggccgaggct cgtgatgatt ctgcggagac
      661 gggaccgctc ggcgggtgca gaggggttga agggggcggc aaagggaatg gtggcggtgg
      721 caccgagggg gtcggtggat tcctcgccgc cagtggcgac ccgctcggtc cagcggtcgc
      781 cgtcccacca gcgctgctgg gcccggccgt aggggtcggg tcgccattgg gctggcatct
      841 gctccccgat catctcatcg ctcggcagtc cctcgtcggt catgttcgtc cccctcacct
      901 tgccgtgggc gattctggca tccccgggtc aggggtcgcc gcgatgctcg gggtacctgc
      961 cgacggcggg gtcgtcacgc catactgttg cccatcgcca ccgttccccc gaccacgcgg
     1021 gcccctcgtc ggaacccggc tcacatcagt cgcgtgccgt atctgcccgg cctcgacggc
     1081 atgcgcgcgc tggccgttgt ggccgtgatg atcttccacg ccaaccacgc ctggatgccc
     1141 gcggggttcc tgggcgtcga agtgttcttc gtcatcagcg gatacctcat caccctgctg
     1201 ctgattgcag aggacgagcg cgacggcggc gtctcgctgc gcgccttttg gggacgacgg
     1261 ttccgccgcc tgctccccgc gttgtacctc accctgatcg gcctcgtgat ctactgcgcg
     1321 ctgttcatgc gcgagcaact cggccacctg cgcggcgacg tggtggcggc ggtgttctac
     1381 gtcagcaact ggttccagat ctgggtcggg cagggctacg gctcgctgac cgcgttcgtg
     1441 cccctgcgcc acctgtggtc gctggcggtc gaagagcagt tctacctggt gtggccgttg
     1501 gtgatggtgt ggctactgcg caagggtcgc gagcggctgc cccaggtcgg cctgcaactg
     1561 atcgcggcgg cgttcgtgat cgcggcggtc acgtcgatct tgttccacaa gggcgtggtc
     1621 agcggtgtcg aatcgacccc cgacgcgtac tggtcgttgt tcggccgcga catcgacacc
     1681 aacaacgcgc tgtacctgtc gaccattacc cgggccagcg gcatcctgct cggggccggg
     1741 ttcgcgatgt tgtggcgccc ggtggcgatg atgcgtgggc cgatgcgcac caagggccgc
     1801 cagctcgacc tgatcgccgc ggtcgcgctg gtgctgctgg tcggccagat gctgacgtac
     1861 gagctgatcg gtgaacggac ggcccgctac ttcccgccgc tgttcaacgg cggcctgctg
     1921 ctgacgggcg tgttgacggt ggtgctgatc gctgccgtca cgcacagcga gacgttgatc
     1981 agcaagctgc tcggccaccc ggtgttgaac tggatcggca cccgcagcta cgggctgtac
     2041 ctgttccann nnnnnnnnga tcatccgcaa gcaggccggg ctggcattgt cgctgccgaa
     2101 gttcgtggtt gccctcgtgc tgaccgggtt ggtcaccgag gcgagctatc ggttcctgga
     2161 gatgccggta cgccgccgcg agttcttcca gcgactgcgc actccgacgc cacaggtgct
     2221 gatgggcatg gccgcggtgg cgttgctgac cggctacggc ggcgtgtcgt tgctgacggc
     2281 agacctgcgt tgcaccacca agatcgagtg cgacgcggaa gcagcccgcg acgcgctggc
     2341 gcagtcgacc accacgacgc aaaccggcaa cacacagacc agcaccaccg gcacgccgaa
     2401 cagcgatgcg acgggcaaca gcgatgccac cacgacgtcg accacggcgg cggtgcgcca
     2461 gattcctnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn ncgacgccgc cgagagccgc ggtcccaact cggtgctgac
     2581 acaggtgcag gcctacgccg caccgtccaa ccaggtcgac gtgatggtga tccaagtcgg
     2641 caccaacggt gcggtcaccc aggctcagta cgacgcgatc gccgccgcgg ccgcgccggt
     2701 cggcaaggtg ttcttcatga cgatcaagac cccgtacgac tacaccgagc cgaacaacgc
     2761 gatcatccgt gccctgccga acacgtaccc caacgtgcag atcatcgact ggcagaccga
     2821 ggcggccaag gtcgaagacg gcttgtcgaa ggccgacggc ggcatccacc tcaacagcgg
     2881 cacagccgtg cggttctacg ccaacctgat cctgaccgcg gctggtctgg agccgatccc
     2941 cgacccggac agtggtggtg gctgaccgcc gcgagcgact gtcgcgcttc gggttcagac
     3001 ggatttccgt cgggcacgcg gcggccctac gctttcgccc atgacatctc cactccgtgt
     3061 tgccgtttcc ggcgctgctg gccagatcgg ctacagcctg ctgttccgca tcgccagcgg
     3121 cgaaatgctg ggcaaggacc agcccgtcat cctgcagttg ctcgacctgc cgcaagccca
     3181 gaaggccgtc aagggcgtga tnnnnnnnnn nctcgacgac tgcgccttcc cgctgctggc
     3241 cgacacggtg tgcaccgata gcgccgatgt cgccttcggt gacgccgacg tggtgttgct
     3301 ggtcggcgca atgccgcgca aggcgggcat ggagcgcagc gacctgctct ccgccaacgg
     3361 tggcatcttc aagccgcagg gcgaagcgct cagccgcagc gccaagaagg atgtcaagat
     3421 cctggtggtc ggcaacccgg ccaacaccaa cgcgctgatc gcgatgcaca acgccaagga
     3481 tctcgacccc ggccgcttca ccgcgatgac c
//
LOCUS       DAORNF010000324         3491 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_20308_length_3491_cov_1.901339, whole genome shotgun sequence.
ACCESSION   DAORNF010000324 DAORNF010000000
VERSION     DAORNF010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3491
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20308_length_3491_cov_1.901339"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(549..1832)
                     /gene="nusA"
                     /locus_tag="PLV68_12770"
     CDS             complement(549..1832)
                     /gene="nusA"
                     /locus_tag="PLV68_12770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HRE02168.1"
                     /translation="MSGLDMAEAVRMLAADKGISVDTLLQVLADALVSAYKRRAGAAE
                     EAEVQIDADTMDIRIIAYDVDEDGNWVNGRDDTPDDMGRIAAQTFRQVLNQRIREAER
                     DRKFEEYANREGDIVTGIIQQTDSRYTLLDLGRVEALLPQAEQVQFERPEPGGRVKAY
                     IVEVRKTAKGPQIVVSRTHPGLIKRLFELEVPEIADGVVEIKACAREPGHRTKIAVWS
                     NDSNVDPVGACVGARGGRVRMVVNELRGEKIDIVPFSEDFPDFIAKALSPAKVTQVIV
                     AADGTQADVIVPDNQLSLAIGKEGQNARLAARLTGVRIDIRGESQPIDEIDEPEYEAA
                     DYAEGEWRENTTTGQMEFHSPDGTVVSADEWEQGATVVPDEATGEAGPDPDAPVGDPP
                     PANDTLLHSETDATLFADVVDESADPESDGDGDNT"
     gene            complement(1829..>2063)
                     /locus_tag="PLV68_12775"
     CDS             complement(1829..>2063)
                     /locus_tag="PLV68_12775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02169.1"
                     /translation="ASAAADTSGSGERVISYDMIDRAKTVFVWEKSDKVSLKPKAGAG
                     KTSAGKTSAGKNGSAMPGTTQNDTTQNDTEVTA"
     assembly_gap    2064..2073
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2074..>2403)
                     /locus_tag="PLV68_12780"
     CDS             complement(<2074..>2403)
                     /locus_tag="PLV68_12780"
                     /inference="COORDINATES: protein motif:HMM:NF036577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02170.1"
                     /translation="ITVDTPAGTVSGGESDLAPESRHGGVTLDQLALITRLIGREFDH
                     HDPIAHHYTLEVSSPGLERALRTPAHFQREVGKVVNVRMRAITDGVRRVQGVLVAADD
                     DTITVRLD"
     assembly_gap    2404..2427
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2756..>3491
                     /gene="proS"
                     /locus_tag="PLV68_12785"
     CDS             2756..>3491
                     /gene="proS"
                     /locus_tag="PLV68_12785"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HRE02171.1"
                     /translation="MSTLFLRTLREDPADAEVASHRLLVRAGYIRRAAPGGYTWLPLG
                     WIVFRNVERIIRDEMDAAGFQEVHFPALLPREPYEITNRWNDYGPSLFRLVDRRGADY
                     LLAPTHEEMFTLLVKDLYSSYKDLPVSLYQIQTKYRDEARPRAGLLRGREFVMKDSYS
                     FDIDDEGLQVSYDKHRVAYQRTFDRLGMPYVIVSAMSGAMGGSASEEFLAPLAVGEDT
                     YVRCTTCDYAANTEAVQVQPPAAIPLD"
BASE COUNT          490 a         1159 c         1172 g          636 t
ORIGIN      
        1 cgtcgcgctc ggcgcggcgg cgcaggcggt cggcctgctg ttcgatgatg gtggatgaat
       61 ggttcttgga gccgacgccc atgacgtcgg ccagttccat gatctctacg ttggtcatgc
      121 caagctcttt ggcgagctcg tgcatccgga tgctcttcga caagaccggt acagcccttt
      181 cgtggtggtg tgctgaaccg gcggtgcgtc cggtcagcgg ggatcagtgt tccatgttcg
      241 tcttcacatc acacgcggca atcccagagt ttgtgacctg ggggctggtc tgtgaccagt
      301 cgccgagcgt gcgctcgagg tgtcgtgtca tgttcgcgtc gagcgggcgc ttccacgccc
      361 ggtcgaaccc gcgtcgtttg agtgcctgag ctccacaggc tggagcagtc gttgcgcaca
      421 accatgcgcc gcgtcccact gcggtgcggc tgacgatggg ggcatcgccg gccaacgcca
      481 gacgcacgag ctgcggctgc ggacgcttgg tccggcagcc gacgcacgtg cgcaccggca
      541 ccacagcgtc aggtgttgtc gccatcacca tcactttccg ggtcggcact ttcgtcgacg
      601 acgtcggcga acagggtcgc gtcggtctcg ctgtgcagca gcgtgtcgtt ggccggcggc
      661 gggtcgccga ccggcgcgtc ggggtcgggg ccggcctcac cggttgcctc gtcgggcacc
      721 accgtggcac cctgctccca ctcgtcggcg ctgacgacgg tgccgtcagg gctgtggaac
      781 tccatctggc ccgtggtcgt gttctcccgc cactcgccct cggcgtagtc ggcggcttcg
      841 tattcgggct cgtcgatctc gtcgatcggc tggctctccc cgcggatgtc gatgcggacg
      901 ccggtgaggc gggcggccag gcgggcgttc tggccctcct tgccgatcgc cagtgagagc
      961 tggttgtcgg gcacgatcac atcggcctgg gtgccgtcgg cggcgacgat cacctgggtg
     1021 accttggccg gcgacagcgc cttggcgatg aagtcgggaa aatcctcgct gaaggggacg
     1081 atgtcgatct tctcgccacg cagctcgttg accaccatgc gcacacgtcc accgcgggca
     1141 ccgacgcagg caccgaccgg gtcgacgttg ctgtcgttgc tccacaccgc gatcttggtg
     1201 cggtgccctg gctcgcgggc gcaggccttg atctcgacca cgccgtcggc gatctcgggc
     1261 acctccagct cgaacagccg cttgatcaga ccggggtggg tacggctgac cacgatctgg
     1321 gggcccttcg cggtcttgcg gacctcgacg atgtaggcct tgacccggcc gcccggctcg
     1381 ggacgctcga actgcacctg ctcggcctgg ggcagcagcg cctcgacacg gccgaggtcg
     1441 agcagcgtgt agcggctgtc ggtctgctgg atgatgccgg tgacgatgtc gccctcgcgg
     1501 ttcgcgtact cctcgaactt gcggtcgcgc tcggcttcgc ggatgcgctg attgagcacc
     1561 tggcggaagg tctgcgcggc gatgcggccc atgtcgtcgg gcgtgtcgtc acggccgttg
     1621 acccagttgc cgtcctcgtc gacgtcgtag gcgatgatgc ggatgtccat cgtgtcggcg
     1681 tcgatctgca cctcggcctc ttcggcagca ccggcgcgac gcttgtatgc gctgaccagc
     1741 gcgtcggcca acacctgcag caaggtgtcg acggaaatcc ccttgtcggc cgcgagcatg
     1801 cggacggctt cggccatgtc gagaccactc acgcggtcac ctccgtgtcg ttctgggtcg
     1861 tgtcgttctg tgttgtgccg ggcatcgcgg acccgttctt gccagcactc gtcttgccag
     1921 cactggtctt gccagcgccg gccttgggct tgagcgacac cttgtcgctc ttctcccaga
     1981 cgaacaccgt cttggcccgg tcgatcatgt cgtacgagat gacccgctcg ccgcttccgg
     2041 acgtgtcggc cgcagccgac gcgnnnnnnn nnnatcgagg cgcaccgtga tcgtgtcgtc
     2101 atcggcggca acgagcacac cctgcacccg gcggaccccg tcggtgatcg cgcgcatgcg
     2161 cacgttgacc accttgccca cctcacgctg gaagtgggcc ggagtgcgca gcgcccgctc
     2221 gaggccgggg ctcgacacct ccaaggtgta gtggtgggcg atggggtcgt ggtggtcgaa
     2281 ctcgcgcccg atcaggcggg tgatcagcgc gagttgatcg agcgtgacgc cgccgtgacg
     2341 cgactccggg gcgagatctg actcgccgcc ggacaccgtg cccgcgggcg tgtcgacggt
     2401 gatnnnnnnn nnnnnnnnnn nnnnnnngta cagatcgagg tgcaggcccg tcaccacggg
     2461 agtgacgagc gcgacgatgc gatcggagac ggctgctggg gtcgttgcgg gcatcgtggt
     2521 cacctcactc tcatcgggat tgctggctgt gttggctggt tgcggactgg ccccggacga
     2581 aacaaaaggc gtgggccgag acccacgcct ttgtcgttgt gcgaacttcg ggcacagcga
     2641 ggataccagc cagataccct ccttgggcgc cgcaacctgc ggcgtcggcc ctgccgccac
     2701 cggttcgtgc atcggccggg ttcgtgtctg tatcgagagg tccaccgtgt tgcgaatgtc
     2761 gacgttgttc ctgcgcacct tgcgcgagga cccagccgac gccgaagtcg ccagccatcg
     2821 cttgctggtg cgggccggct acatccgccg cgccgcgccg ggcggctaca catggctgcc
     2881 gctcgggtgg atcgtgttcc gcaacgtcga gcggatcatc cgcgacgaga tggatgcggc
     2941 cgggttccaa gaggtgcact tccccgcact gttgccccgc gagccgtacg agatcaccaa
     3001 ccgctggaac gactacggcc cgagcctgtt ccgcctggtc gaccgccgcg gcgccgacta
     3061 cctgctcgcg cccacgcacg aagagatgtt cacgctgctg gtcaaagacc tgtacagcag
     3121 ctacaaggac ctgccggtgt cgctgtacca gatccagacc aagtaccgcg acgaagcccg
     3181 tcctcgtgcc gggctcctgc gcggccgcga gttcgtgatg aaggactcgt acagcttcga
     3241 catcgacgac gagggcctgc aggtcagcta cgacaagcac cgcgtcgcct accagcgcac
     3301 cttcgaccgg ctcggcatgc cgtacgtgat cgtgtcggcg atgagcggcg cgatgggtgg
     3361 ctcggccagc gaggagttcc tcgcaccgct ggccgtcggc gaggacacct acgtgcgctg
     3421 caccacctgc gactacgccg ccaacaccga ggccgtccag gtgcagccgc ccgcggcgat
     3481 cccgctcgac g
//
LOCUS       DAORNF010000325         3488 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_20332_length_3488_cov_1.748616, whole genome shotgun sequence.
ACCESSION   DAORNF010000325 DAORNF010000000
VERSION     DAORNF010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3488
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20332_length_3488_cov_1.748616"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..853
                     /locus_tag="PLV68_12790"
     CDS             <1..853
                     /locus_tag="PLV68_12790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRE02172.1"
                     /translation="AGVFNLVSGIGPVVGEAIAAHPDVDMVSFTGSTRAGKRVAQVGA
                     ETVKRVALELGGKSANILLDDLDTDGFGKAVSDGVRKAFFNSGQTCSALTRMLVPRDR
                     LAEAEALAGATADALVVASPFADGVHLGPLASAAQRDRVQGYIQKGLDEGATLVAGGL
                     GAPEGLDVGYFVKPTVFSNVSPNMTIAQEEIFGPVLSILPYDDEDDAVRIANGVVYGL
                     AGGVWSADKDRAVAVARRLRTGQVEVNGGAFNPNAPFGGYKQSGIGREYGTFGLEEFL
                     EIKSMQL"
     gene            948..>1533
                     /locus_tag="PLV68_12795"
     CDS             948..>1533
                     /locus_tag="PLV68_12795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02173.1"
                     /translation="MAVACVYCGGSHDSPAGVRECWQSRQGREHAGTATQPSFDGPMS
                     DAIDVGDSADVVYGVDAAPPDAPPRPASGAAPSVFGAPGPDALARNLVVASPDAVVPA
                     PWTNAERVVVDDAVLAGPEPVVARLTALAAAGTRTVIELAVPFPGPLDPPRSTTTDAP
                     YRLGARFWFPHDVLHHVVWANAIDHTGPRPSWWAV"
     assembly_gap    1534..1621
                     /estimated_length=88
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1622..2591)
                     /locus_tag="PLV68_12800"
     CDS             complement(<1622..2591)
                     /locus_tag="PLV68_12800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02174.1"
                     /translation="MAATVVTHEPIGCFDHVARAAVVDLERMRGCRRKQLREVDQPGR
                     IGPAVAVDRLVVVADAEHRQTRRRQQPHQQHVRGCEVLEFVDQQHAAGTLGGGPGIGV
                     GEQNLQCRVHLLVEVDHPVAAQCGAILRPHLDQAVDFAVVTALDGHRVDQAQTDRAQR
                     FDPHRDRVGVAFARKLHQPPDDAPHLEFVDGAHLLASWSKRRRAVDDRQRDRVEGANL
                     QPAQVAGARPHLFLGTLVERHQADGRGRQPPFTQQVARPLGEHAGLAAASGSDDACRA
                     TMVSDRSELIGGQIGGGRGIAGRDERAMFESDRVHDADPADRGHPAH"
     gene            2626..>2998
                     /locus_tag="PLV68_12805"
     CDS             2626..>2998
                     /locus_tag="PLV68_12805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02175.1"
                     /translation="MRVDRATEADLGDMGDTADTDEPEAANETSTLQRSLDAVTDAIA
                     AGRASADIAVLTRVNALLAPIQVGLMARGVAVRGGVGRDFAERTAVRAVLAWLRLAGA
                     TGDTLDAADLGEALKRPSRPLH"
     assembly_gap    2999..3163
                     /estimated_length=165
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3164..>3488
                     /locus_tag="PLV68_12810"
     CDS             <3164..>3488
                     /locus_tag="PLV68_12810"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRE02176.1"
                     /translation="EIGLGASIAGLDLGRQGMNRAAQNDDLVALAQLAELHPDVVTFE
                     AWLRRSLAQPLDDAGVVLATVHRVKGQEWPTVVLHHAEVDQYPHRLAEDLEEERRVFH
                     VAITRA"
BASE COUNT          461 a         1123 c         1159 g          492 t
ORIGIN      
        1 cgccggcgtg ttcaacctgg tcagcggcat cggcccggtc gtgggcgagg cgatcgcggc
       61 ccacccggac gtcgacatgg tcagcttcac gggctcgacg cgggccggca agcgtgtcgc
      121 ccaggtcggc gcggagacgg tcaagcgggt cgccctcgaa ctcggtggca agagcgccaa
      181 catcctgctc gacgacctcg acacggacgg cttcggcaag gcggtctccg acggtgtccg
      241 caaggcgttc ttcaacagcg gccagacctg ctcggcgctg acccgcatgc tcgtgccccg
      301 cgaccgcctg gccgaagcgg aagcgctcgc cggtgccacc gcggacgcgc tcgtggttgc
      361 gagccccttc gcggacgggg tgcacctcgg cccgctcgcc tcggctgccc agcgtgaccg
      421 tgtccagggc tacatccaga agggtctcga cgaaggcgcg accctggtcg cgggtggcct
      481 cggcgcgccc gagggtctcg atgtcggcta cttcgtcaag ccgacggtgt tcagcaacgt
      541 gtcgccgaac atgaccatcg cccaggaaga gatcttcggg ccggtgctgt cgatcctgcc
      601 ctacgacgac gaggacgacg ccgtgcgcat cgccaacggc gtggtctacg gcttggccgg
      661 cggggtgtgg tcggccgaca aggaccgcgc cgtggccgtg gcccggcgcc tgcggacggg
      721 tcaggtcgag gtcaacggtg gggcgttcaa tcccaacgcg cccttcggtg ggtacaagca
      781 gtcgggcatc ggccgcgagt acggcacctt cggtttggaa gagttcctcg agatcaagag
      841 catgcagctg tagtcactgc ccaatggggg agcgcgcgaa gggggccggc tctgtgacac
      901 ccgctgatct cgcgatgact gtgacaccca tcgggtagaa catctgtatg gctgtggcat
      961 gtgtgtactg cggcgggtcg cacgactcgc ccgccggggt gcgcgagtgc tggcaatccc
     1021 gccagggccg cgagcacgcc ggaaccgcca cgcagccatc gttcgacggg ccgatgtccg
     1081 acgcgatcga cgtgggggac tcggcggacg tcgtctatgg ggtggatgcg gcgccaccag
     1141 acgcgccacc gcgcccggca tccggtgcgg caccgagtgt gttcggcgca ccagggccag
     1201 atgcgttggc ccgcaacctg gtggtcgcct cgcccgacgc ggtggtgccc gcaccgtgga
     1261 ccaatgccga gcgggtcgtg gtcgacgatg ccgtcctggc tggacccgag cccgtggtgg
     1321 cgcgcctgac cgcgctcgcc gccgctggga cgcggaccgt gatcgaactg gccgtcccgt
     1381 tccccggtcc gctcgacccg ccgcgctcga ccaccaccga cgcgccgtat cgcctcgggg
     1441 cgcggttctg gtttccgcac gacgtgctgc accatgtggt gtgggcaaac gccatcgacc
     1501 acaccgggcc gcggccgtcg tggtgggccg tcgnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nggtgcgccg gatggccccg atcggcgggg tcggcgtcgt gcacacggtc gctctcgaac
     1681 atggctcgct cgtcgcgccc agcgatgccc cgcccgccgc cgatctggcc cccgatcagc
     1741 tcgctgcggt cgctcaccat ggtggcgcgg cacgcatcat cgctcccgct ggcagcggca
     1801 agacccgcgt gctcaccgag cgggcgcgcc acctgctgcg tgaatggcgg ctgccggccg
     1861 cggccgtcag cctggtggcg ttcaacaagc gtgcccagga agagatgcgg tcgcgcaccc
     1921 gcgacctgcg cgggctgcag attcgcaccc tcaacgcgat cgcgctggcg atcgtcaacg
     1981 gcacggcgcc gtttgctcca cgacgccagc aggtgcgcac catcgacgaa ttcgaggtgc
     2041 ggcgcatcat cgggcggctg gtgcagtttc cgcgcaaacg caacaccgac ccgatcgcgg
     2101 tgtggatcga agcgctgagc acggtccgtc tgggcctggt cgacccggtg accgtcgagg
     2161 gccgttacaa cggcgaagtc gacggcttgg tcgaggtgtg gccgcagtat cgcgccgcac
     2221 tgcgccgcga cggggtggtc gacttcgacg agcaggtgta cgcggcactg gagattctgc
     2281 tcgccgacgc cgatgcccgg gccgccgccc agcgtgcctg ccgcatgctg ctggtcgacg
     2341 aattccaaga cctcacaccc gcgcacgtgc tgctggtgcg gttgctgtcg gcgccgggtc
     2401 tggcggtgtt cggcgtcggc gacgacgacc agacgatcta cggctacagc ggggccgatc
     2461 cggcctggct gatcgacttc gcggagctgt ttccgtcggc agcctcgcat ccgctcgagg
     2521 tcaactaccg ctgccctggc gacgtggtcg aagcagccga tcggctcgtg cgtcacaacc
     2581 gtcgccgcca agacgatccg cagcgcccac gccggtcggc gtggcttgcg cgtcgaccgg
     2641 gccaccgagg ccgacctggg ggatatgggc gatacggccg acacggacga gccggaggcg
     2701 gcgaacgaga cgtcgacgtt gcagcgcagc ctcgatgcgg tgaccgacgc gatcgctgcc
     2761 ggacgggcat ccgccgacat cgccgtgctg acccgcgtca acgcgttgct ggcgccgatc
     2821 caggtcggcc tgatggcgcg gggcgtcgcg gtgcgcggcg gtgtcggacg cgacttcgcc
     2881 gagcgcaccg cggtgcgggc tgtgttggcg tggctgcgcc tcgccggagc gacgggtgac
     2941 acgctcgacg cggccgacct cggtgaggcg ctcaagcgcc cgtcacggcc gctccatcnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncgagatc ggtctcggtg
     3181 cttcgatcgc agggctcgac ctcggtcgcc agggcatgaa ccgggccgct cagaacgacg
     3241 atctggtcgc gttggcccag ttggccgagc tgcatcccga cgtcgtcacg ttcgaagcct
     3301 ggctgcgccg ctcgctggcc cagccactcg acgacgccgg tgtcgtgctg gccaccgtgc
     3361 accgggtgaa gggacaggaa tggccgaccg tggtgttgca ccacgccgag gtcgaccagt
     3421 atccgcaccg cctggccgaa gatctcgaag aggagcggcg cgtcttccat gtcgcgatca
     3481 cgcgagcg
//
LOCUS       DAORNF010000326         3469 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_20478_length_3469_cov_2.038371, whole genome shotgun sequence.
ACCESSION   DAORNF010000326 DAORNF010000000
VERSION     DAORNF010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3469
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20478_length_3469_cov_2.038371"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="PLV68_12815"
     CDS             complement(<1..199)
                     /locus_tag="PLV68_12815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02177.1"
                     /translation="MTDEATPAEHPSGYHLAHQYLAAAFFDLDRTLISGSSAFVFGIA
                     AWRADMLPSRQFVKDAAGALAF"
     gene            complement(240..316)
                     /locus_tag="PLV68_12820"
     tRNA            complement(240..316)
                     /locus_tag="PLV68_12820"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(280..282),aa:Pro,seq:tgg)
     gene            complement(407..676)
                     /locus_tag="PLV68_12825"
     CDS             complement(407..676)
                     /locus_tag="PLV68_12825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02178.1"
                     /translation="MTYWKPHALAKPHQDQLDLRIGDHVSNTVELEGVPVGTTGRVIL
                     ANGFNWQRYRVLFDNGAEFGDLDGRQIEPSGKTAKRLAKAAAKRR"
     gene            817..>1124
                     /locus_tag="PLV68_12830"
     CDS             817..>1124
                     /locus_tag="PLV68_12830"
                     /inference="COORDINATES: protein motif:HMM:NF017508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor family protein"
                     /protein_id="HRE02179.1"
                     /translation="MKSTVEPLEGNKVKVSVTVDESEFDRDIDAAFRKIAHEVRLPGF
                     RAGKAPRRVLEARIGVAAAREQALRDGVPNYLAKAVREHDVDLIATPSVEITGGEDAG
                     "
     assembly_gap    1125..1141
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1142..>1620
                     /locus_tag="PLV68_12835"
     CDS             <1142..>1620
                     /locus_tag="PLV68_12835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02180.1"
                     /translation="EVSVAGYGGLRVELPAVTASDDDVDAAVNAELSRQATLADIDRP
                     VAFGDQVTLDLAASIDGEPVPGLNTDEWLYEVGKGWVAGGFDDQLVGASAGDELSFRL
                     VPNGAEAEADFAVTVSRVQEKVLPELTDTWVDENIAEFDTVEAWRASVRERLDQTKL"
     assembly_gap    1621..1659
                     /estimated_length=39
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1660..>1926
                     /locus_tag="PLV68_12840"
     CDS             <1660..>1926
                     /locus_tag="PLV68_12840"
                     /inference="COORDINATES: protein motif:HMM:NF017509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02181.1"
                     /translation="ELVDVEPPEAMVQGDLQQRVQNTVQQFQAQGIAIDQFLSITGQT
                     SEQFVETLRTQSHQAVKVDLALRAVAAAEGIEIDADEIEAEFQRI"
     assembly_gap    1927..2147
                     /estimated_length=221
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2198..>2541
                     /locus_tag="PLV68_12845"
     CDS             2198..>2541
                     /locus_tag="PLV68_12845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HRE02182.1"
                     /translation="MTSPLLQPVYDYLVPNVIETTSRGERHSDLYSRMLRENIIFLAT
                     PVDDTVANLICAQLLHLESENPEKDINIYINSPGGDITALFAIYDTMQFVRNDIATIC
                     LGQAASAAAVLLA"
     assembly_gap    2542..2577
                     /estimated_length=36
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2578..2859
                     /locus_tag="PLV68_12850"
     CDS             <2578..2859
                     /locus_tag="PLV68_12850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HRE02183.1"
                     /translation="RLALPHARVLLHQPWGQVGYGQVTDLELAAKEILRMRDLLEEIL
                     AKHTGQSIERIHQDTDRDFVIEAEAAVEYGIIDEVITSRLAVDRTGPIR"
     gene            2891..>3469
                     /locus_tag="PLV68_12855"
     CDS             2891..>3469
                     /locus_tag="PLV68_12855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRE02184.1"
                     /translation="MAKFGDTGELLKCSFCGKSQKQVKKLIAGPGVYICDECIDLCNE
                     IIEEDLTETSELRFEDLPNPREIRAFLDDYVVGQESAKKILSVAVYNHYRRIQHQQNT
                     GVGRRDEVELAKSNILMLGPTGCGKTHLAQTLARMLNVPFAVADATALTEAGYVGEDV
                     ENILLKLIQAADFDVKKAETGIVYIDEIDKVAR"
BASE COUNT          580 a         1036 c         1038 g          502 t
ORIGIN      
        1 gaaacgccaa ggctccggcg gcgtccttga cgaactggcg gctgggcagc atgtcggccc
       61 gccaggcggc gatgccgaac acgaacgccg acgagccgct gatcagcgtg cggtcgaggt
      121 cgaagaacgc cgcggcgagg tactggtgcg ccaagtggta tccgctgggg tgctcagctg
      181 gggttgcttc gtcggtcatg ggggtggtgc cgatcgtggt gcaatcgcgg tggggaaggt
      241 ggtcggaacg ccgggatttg aacccgggac cccctgctcc caaagcaggt gcgctaccaa
      301 gctgcgctac gttccgttca ttcgtggccg tgacggccag cggtcattcg tggccgtgac
      361 ggccggaggt cagcttaccc gtgagcgggg gacagccggg gggagttcag cgccgcttgg
      421 cagcggcttt ggcgaggcgc ttggcggtct tgcccgacgg ctcgatctgg cgtccgtcga
      481 gatcgccgaa ctcggcaccg ttgtcgaaca gcacgcgata gcgctgccag ttgaagccgt
      541 tggcgaggat gacccggccc gtggtgccga ccgggacgcc ctcgagttcg acggtgttgg
      601 agacgtggtc gccgatgcgc aggtcgagct gatcctgatg cggcttggcg agggcgtggg
      661 gcttccagta cgtcacgccg acattctgcc cggcctgcgt cggtgttgac gactttcact
      721 gttgcgacgt tcaccgttga cgactttctc tggggcgacg gctcggacgg tgcggccagg
      781 cggctggcgt tttccggccg atacactcgg ccacctgtga agagcactgt cgaacccctc
      841 gagggcaaca aggtcaaggt gtcggtcacc gttgacgaat ccgagttcga tcgcgacatc
      901 gacgccgcat tccgcaagat cgcccacgag gtccgcctgc ccggtttccg cgccggcaag
      961 gcgccgcgtc gggtgctcga agcgcgcatc ggtgtggctg cggctcgcga gcaggccctc
     1021 cgtgacggcg tgcccaacta cttggccaag gccgtgcgcg agcacgacgt cgatctcatc
     1081 gccacgccgt cggtcgagat caccggggga gaagacgccg gcgannnnnn nnnnnnnnnn
     1141 ncgaggtcag cgttgccggt tacggcggcc tgcgcgtcga gctgcccgcg gtcaccgcca
     1201 gcgacgacga cgtcgacgcc gcggtcaacg ccgagttgtc gcgccaggcg acgctggccg
     1261 acatcgaccg gcccgtcgcg ttcggcgacc aggtcacgct cgatctggca gcgagcatcg
     1321 acggtgagcc ggtgcctggg ctgaacaccg acgagtggct gtacgaggtc ggcaagggct
     1381 gggtcgccgg tggcttcgac gaccaactcg tgggcgccag cgccggcgac gagctgtcgt
     1441 tccgcctcgt gcccaacggt gccgaggccg aggccgactt cgctgtcacc gtgtcgcggg
     1501 tgcaagagaa ggtgctgccc gaactgaccg acacgtgggt cgacgagaac atcgccgagt
     1561 tcgacacggt cgaggcatgg cgcgcgtcgg tgcgcgagcg gctcgaccag accaagctca
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng aactggtcga cgtcgagccg
     1681 cccgaggcca tggtgcaggg cgacctgcag cagcgggtgc agaacacggt gcagcagttc
     1741 caggcccagg gcatcgccat cgatcagttc ctgtcgatca ccggccagac cagcgagcag
     1801 ttcgtcgaga cgctgcgtac ccagtcgcat caggcggtca aggtcgatct cgccctgcgc
     1861 gccgtcgccg ctgccgaagg catcgagatc gacgccgacg agatcgaggc cgagttccag
     1921 cgcatcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngac cacagcgatc
     2161 acgaccacag cgatcacggt cacgaggagg accactgatg accagccccc tgttgcagcc
     2221 ggtgtacgac tacctggtcc ccaacgtcat cgagaccacc agccgcggtg agcgtcattc
     2281 cgacctctac tcgcggatgc tgcgcgaaaa catcatcttc ctcgccactc cggtcgacga
     2341 caccgtcgcc aacctgatct gtgcacagct gctccactta gagagcgaaa atcctgaaaa
     2401 ggacatcaat atctacatca acagccccgg tggcgacatc accgcgctgt tcgccatcta
     2461 cgacacgatg cagttcgtgc gcaacgacat cgccacgatc tgtcttggtc aggcagcctc
     2521 agccgcagca gtgctgctgg cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncgc
     2581 ctcgcactgc cgcacgcccg ggtgctgctg caccagccgt ggggccaggt cggctacgga
     2641 caggtcaccg acttggagct ggccgccaag gagatcctgc gcatgcgcga tctgctcgaa
     2701 gagatcctcg ccaagcacac cgggcagtcg atcgagcgca tccaccaaga caccgaccgc
     2761 gacttcgtga ttgaagccga ggcggctgtc gaatatggca tcatcgacga agtgatcacg
     2821 tcccgcctgg cagtcgaccg caccggtccg attcgctgac cggctgacga gcgagcagaa
     2881 ggggaccccg ttggccaagt tcggtgatac cggcgaactg ttgaagtgtt cgttctgcgg
     2941 caagtcgcaa aagcaggtca agaaactgat cgctggcccc ggggtctaca tctgcgacga
     3001 gtgcatcgat ctctgcaacg agatcatcga ggaagacctc accgagacca gcgagctgcg
     3061 gttcgaagac ctgcccaacc cccgcgagat ccgggccttt ctcgacgact acgtggtcgg
     3121 gcaagagagc gccaagaaga tcctctcggt cgcggtctac aaccactacc ggcgcatcca
     3181 gcaccagcag aacaccgggg tcggccgccg cgacgaggtc gagttggcca agagcaacat
     3241 cctcatgctc ggccccaccg ggtgcggcaa gacccacctc gcccagaccc tggcgcgcat
     3301 gctcaacgtg cccttcgccg tcgccgacgc caccgcgctc accgaagcgg gctacgtcgg
     3361 cgaagacgtc gagaacatcc tgctcaagct gatccaggcc gccgacttcg atgtcaaaaa
     3421 ggccgagacc ggcatcgtct acatcgacga gatcgacaag gtggcgcgc
//
LOCUS       DAORNF010000327         3459 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_20557_length_3459_cov_1.729730, whole genome shotgun sequence.
ACCESSION   DAORNF010000327 DAORNF010000000
VERSION     DAORNF010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3459
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20557_length_3459_cov_1.729730"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(69..>876)
                     /locus_tag="PLV68_12860"
     CDS             complement(69..>876)
                     /locus_tag="PLV68_12860"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HRE02185.1"
                     /translation="LVPLARDAEALGFDAVSVGDSIVYPRRSDVKYPYTATGDRSFIE
                     GQPFIDPIVAGAAVLTATTTLSFQSSVLKLGVRHPVVVAKQVASLAALAPGRVRLGVG
                     SSPWPEDFTIMGLTPQRRGSRLAEAIEVVQRLLQGGYQSYSGEFYELPEARMDPTPPI
                     PVPILIGGHGSANLRRAATLGDGWIAAGADQSRLGDMIAELHSHRRDAGRAEEAFAVH
                     APLHGDAGAIDHLLALGVTNIVVRPGLPGQPSTDLDAARTALAAVASALS"
     assembly_gap    877..972
                     /estimated_length=96
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<973..>1376)
                     /locus_tag="PLV68_12865"
     CDS             complement(<973..>1376)
                     /locus_tag="PLV68_12865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HRE02186.1"
                     /translation="GGAHMTHEAVVAAGPIDQRSVQTRPDVVVWTSAPLSDPVEVTGW
                     IDAELFVGHDADQADYSVVVSDLEPDGLCTNVVDGYRRVHGVRDGEAITVALGATSHV
                     FRAGHAIRVQIAGASSPRHDVVGVAGQPQRQR"
     assembly_gap    1377..1415
                     /estimated_length=39
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1416..>1754)
                     /locus_tag="PLV68_12870"
     CDS             complement(<1416..>1754)
                     /locus_tag="PLV68_12870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02187.1"
                     /translation="DPAHPARDDQYLLMGPWSHRIPRGSTVGQYIVGDQYYGTSAIFD
                     FDTLLLGWMRAVLDGEGRPWPFDGRVRYFTTGINVWRTAPDWPIPGTEKRSLYLDGES
                     LSWAAAPDAA"
     assembly_gap    1755..1791
                     /estimated_length=37
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1792..2563)
                     /locus_tag="PLV68_12875"
     CDS             complement(<1792..2563)
                     /locus_tag="PLV68_12875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HRE02188.1"
                     /translation="MATRPADVVLTRDIGVPMRDGVVLRGDLYLPVGDGPFPCLLQRT
                     PYVKDYNPGIWVVCDPLKAAGAGFAVFIQDVRGRGISDGEFTPFIDEAADGVDTIDWV
                     SRQTWCDGRVAMHGSSYMAAAQWQAAKERPAAFQASCDYYEGRSYRGGAFEIGALLGV
                     SLNALGGGIAHRKIQRGELPKSAAREARTAVDQLVQLARTPLAELRKTVLNDIAPFFF
                     DWAERTDPGHEYWRELDLTGRHQDIHIPVFHLSSWFDQA"
     assembly_gap    2792..2806
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2807..>3027
                     /locus_tag="PLV68_12880"
     CDS             <2807..>3027
                     /locus_tag="PLV68_12880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02189.1"
                     /translation="ARLGASVVVFDKQPAESAGGNTVVSGGVWFHHDDPVAAAAYLRA
                     MNGSAPISADVVEVWARETARNSEWLREL"
     assembly_gap    3028..3071
                     /estimated_length=44
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3072..>3459
                     /locus_tag="PLV68_12885"
     CDS             <3072..>3459
                     /locus_tag="PLV68_12885"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRE02190.1"
                     /translation="SDCYQGYWTVDGVFGGGNLHRFLLRRAVEAGVEVRCASPVSALV
                     RSGETVTGVTLASGATIRARGGVVIATGGFAGSPERVSAHLGIPDASPWGSPHCTGDG
                     LQMAVEVGAGTSHVGRHSRLLGTAVPG"
BASE COUNT          510 a         1093 c         1131 g          494 t
ORIGIN      
        1 cgaaatcgcc ggccgacgac gcctgggggc gccacagcag gtagatgacc ttttccattg
       61 attgctcctc aactgagcgc ggacgcaacg gcggcgagcg cggtgcgggc cgcatcgaga
      121 tctgtcgacg gctgtccggg caggccgggg cgcacgacga tgttggtcac gccgagcgcg
      181 agcaggtgat cgatcgcgcc ggcgtcgccg tgcaagggcg cgtgcacggc gaacgcctct
      241 tcggcgcggc cggcgtcacg gcgatgactg tggagttcgg cgatcatgtc gccgagccgt
      301 gactggtcgg ccccggcggc gatccagccg tctcccaacg tggccgcccg gcgcaggttc
      361 gccgacccgt gcccgccgat gaggatcggc accggtatcg ggggagtcgg atccatgcgc
      421 gcctcgggca gctcgtagaa ctctcctgag tacgactggt agccgccttg tagcagtcgc
      481 tggaccacct cgatcgcctc cgccaagcga gagccacgcc gctgcggggt gaggcccatg
      541 atcgtgaagt cctccggcca cgggctggag ccgacgccga gacgcacgcg accgggcgcc
      601 aacgcggcca gcgatgccac ctgcttcgcg accacgactg gatggcggac accgagcttg
      661 agcactgacg actggaacga cagcgtcgtc gtcgctgtca atacggcggc cccggccacg
      721 atcgggtcga tgaatggctg accctcgatg aacgaccggt cgcccgttgc cgtgtaggga
      781 tacttgacgt cggagcgacg cgggtagacg atgctgtcac cgaccgagac ggcgtcgaaa
      841 cccaacgctt cggcgtcgcg ggcgaggggc acgagcnnnn nnnnnnnnnn nnnnnnnnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      961 nnnnnnnnnn nngcgttgtc gctgcggctg accggccacg ccgacgacgt cgtggcgcgg
     1021 gctggacgcg ccggcgatct gaacccggat ggcgtggcct gcgcgaaaca cgtgcgatgt
     1081 cgctcccagc gcgacggtga tcgcctcgcc gtcgcgaaca ccgtggacgc gccgataccc
     1141 gtcgacgacg ttggtgcaca ggccgtccgg ttcgaggtcg gacacgacca cgctgtagtc
     1201 ggcctggtcg gcatcgtgcc cgacgaacag ctcggcgtcg atccaaccgg tgacctcgac
     1261 cggatccgag aggggtgccg acgtccaaac caccacgtcg gggcgcgtct ggacgctgcg
     1321 ctgatcgatc gggccagccg cgaccaccgc ttcgtgggtc atgtgggccc cgccgcnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntcggc agcgtccggc gcggcggccc
     1441 acgacaggct ctctccgtcc aggtacaacg accgcttctc cgtgccgggt atcggccagt
     1501 cgggtgcggt tcgccacacg ttgatccccg tggtgaagta acgaacccgg ccgtcgaacg
     1561 gccatggcct tccctcgccg tcgagcacgg cgcgcatcca gcccaacaac aaggtgtcga
     1621 agtcgaagat ggccgatgtg ccgtagtact ggtcaccgac gatgtattga ccgaccgtcg
     1681 agccgcgcgg gatccggtgc gaccacgggc ccatcaacag gtactggtcg tcgcgggccg
     1741 gatgggccgg gtcgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngggcctgat
     1801 cgaaccagct cgacaggtgg aagacaggaa tgtggatgtc ctggtgacgt ccggtgaggt
     1861 cgagctctcg ccagtactcg tggcccgggt cggtccgctc ggcccagtcg aagaagaacg
     1921 gggcgatgtc gttgagcacg gtcttgcgca gctccgccaa gggggtccgg gcgagctgga
     1981 cgagttggtc caccgcagtc ctggcctcac gtgcagcgct cttggggagc tccccccgtt
     2041 ggatcttgcg gtgggcgatg cctccaccga gagcattcag cgacacgccc aacagcgcgc
     2101 cgatctcgaa cgcaccaccg cggtaggagc ggccctcgta atagtcgcac gacgcctgga
     2161 aggccgccgg acgctccttg gccgcctgcc actgcgccgc agccatgtag gacgaaccgt
     2221 gcatggccac gcgaccgtcg caccaggttt gtcgggaaac ccagtcgatc gtgtcgacgc
     2281 cgtcggcggc ctcgtcgatg aacggagtga actcgccgtc ggatatccct cgcccgcgga
     2341 cgtcctggat gaacacggca aagccggccc cggctgcctt gagcgggtcg cacacgaccc
     2401 agatccctgg gttgtagtcc ttcacatacg gggtgcgctg cagcaggcat gggaacggcc
     2461 cgtccccgac ggggagatag aggtcgccgc gcagcaccac accgtcgcgc atcggaacac
     2521 cgatatcgcg agttaacacc acgtcagccg gtcgagttgc catctcagga ccgtacgacg
     2581 ccacggtcgg cgggcaaagc gtgctgtccc gttggacggc atcgacgtcc gtgccaccgt
     2641 gcgggaccga tcggcgagcg cggctcgtgt ccgacgcgac aattcagcca tgaccgcctc
     2701 cgaaccatct ccgaccaccg ccgacacaac gcccgtcgac gttgcggtcg acgtcgcggt
     2761 cgtcggcgcc ggagccgctg gtctcgcggc cnnnnnnnnn nnnnnnggcc cgcctcggcg
     2821 cgtccgtcgt cgtgttcgac aagcaacccg ccgagtcggc cggtgggaac accgtggtgt
     2881 ccggtggggt gtggttccat cacgacgacc ccgtcgctgc cgccgcgtat ctgcgggcga
     2941 tgaacggttc ggcaccgatc agcgccgacg tcgtcgaggt atgggcgcgc gaaacggccc
     3001 gcaacagcga gtggctgcgc gaactcgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn naagcgactg ctaccagggc tactggaccg tcgacggcgt cttcggtggc
     3121 ggcaacctgc accggtttct gctccgccgc gcggtcgagg cgggtgtcga ggtccgctgt
     3181 gccagtccgg tctcggcgct cgtgcgcagc ggtgagacgg tgaccggcgt gaccctcgcg
     3241 tctggcgcaa cgattcgtgc tcggggaggc gtcgtgatcg cgaccggcgg cttcgccggt
     3301 agtccggaga gggtgtcggc acacctcggc atccccgatg ccagtccgtg ggggtcgccc
     3361 cactgcaccg gagacgggtt gcagatggcc gtcgaggtgg gtgcgggtac gagccacgtc
     3421 gggcgccatt cgcgcttgct cggcacggcc gttcccggg
//
LOCUS       DAORNF010000328         3458 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_20563_length_3458_cov_1.818689, whole genome shotgun sequence.
ACCESSION   DAORNF010000328 DAORNF010000000
VERSION     DAORNF010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3458
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20563_length_3458_cov_1.818689"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..940)
                     /locus_tag="PLV68_12890"
     CDS             complement(<1..940)
                     /locus_tag="PLV68_12890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase"
                     /protein_id="HRE02191.1"
                     /translation="MTDIDDVDIDVLEQIQQRLLWLAVRIVDSANHDRKPDEVKVGGH
                     QASSASMVGIMTALWFAHLSGNDKVAVKPHASPVFHAIKYLTGELDRSYLTTLRSFGG
                     LQAYPSRTKDPDVADFSTGSVGLGVVAPLFAAATRRYIDAHFRAPSPDGGAPTRARFI
                     ALAGDAELDEGNVWEAIADPSLRGLGNVMWVIDTNRQSLDRVVPEQKIKKLIDFFKGA
                     DWHVVEAKYGAKLHEAFARPGGNLLREYIDAMPNEEYQSMFALTGAALRERFTAGADP
                     RLIQFLGQWNDDEIGPLVQNLGGHDLGPLVAHQPGNG"
     gene            1048..1506
                     /locus_tag="PLV68_12895"
     CDS             1048..1506
                     /locus_tag="PLV68_12895"
                     /inference="COORDINATES: protein motif:HMM:NF013224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HRE02192.1"
                     /translation="MSAASHGGDLDEIDRHIIDILRADGRATFGEIGTTVGLSAPAAK
                     RRVDRLVDRGVIRGFTVMVDHAALGFPLEAFAELRFDGDTAVEEIDAVADDIPEVVGI
                     FTLAGDPDALAWLRVRDVHDLKRVINLIRNTGKVSGTKTLMVLGGSDPKP"
     gene            complement(1511..2926)
                     /locus_tag="PLV68_12900"
     CDS             complement(1511..2926)
                     /locus_tag="PLV68_12900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRE02193.1"
                     /translation="MDTHDTNPAAPQPIDLLVRGWYVVTMNEHRDVIRDGAVAIDGNT
                     IVAVGKAADLARRYAPRRTIGGDRFVVTPGLINTHIHITGEPLTRGYVPDDTPFVENV
                     FEWLTPLYSLFDAAEERTSGQLAAVEMMRSGTTTFLEAGTIRFLDEVIDGLVEVGIRG
                     KVGRWVWDLPPEPAVYRQTTDEAIGHLAHQLEHHRDTGAGRIGPWSIVVGHNTISDPL
                     WKAAKQLAAEYGTGMSFHMSPAMLDPEWYLAEFGQRPIVHLAEIGVLDSNVAMTHCVR
                     VDDSEIALMAEHGAHVAHCPTTALKVSYGVTQVGKMPEMVQRGINVSIGTDGNNASNY
                     SDLARATYLVAGLFKDARTDPQMFPAEVAYEMATMGGARTMQMADQIGSLEVGKKADV
                     VLHDADRPEWRPLLNVVNQLVWSADGRGVHTVVVDGQVTVEDGHCITIDEAKLWADAQ
                     QMGESITARSGLPDKAKFPVY"
     assembly_gap    3096..3250
                     /estimated_length=155
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3251..>3458)
                     /locus_tag="PLV68_12905"
     CDS             complement(<3251..>3458)
                     /locus_tag="PLV68_12905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02194.1"
                     /translation="GAGRAPYALAVLQQSPGSTAVVNDLPGVIELAADTIAAQPDGAD
                     LATRVQYLPGDFHTIGIADGAFDVV"
BASE COUNT          526 a         1109 c         1101 g          567 t
ORIGIN      
        1 gcccattgcc gggctgatga gcgacgagcg ggccgaggtc gtggccgcca aggttctgca
       61 ccagcgggcc gatctcgtcg tcgttccact ggccgaggaa ctggatcaga cgcggatcgg
      121 cgcccgcggt gaaccgctcg cgcagcgcgg cgccggtgag cgcgaacatc gactggtatt
      181 cctcgttggg catcgcgtcg atgtactcgc gcagcaggtt gccgcccggc cgagcgaagg
      241 cctcgtgcag cttggcgccg tacttcgctt cgaccacgtg ccagtcggcg cccttgaaga
      301 agtcgatcag cttcttgatc ttctgctcgg gcaccacccg gtcgaggctc tggcggttgg
      361 tgtcgatcac ccacatcacg ttgccgaggc cgcgcagcga agggtcggcg atcgcctccc
      421 acacgttgcc ctcgtccagt tcggcgtcgc ctgccaaggc gatgaaccgt gcacgcgtcg
      481 gcgcgccacc atcgggcgac ggggcgcgga agtgggcgtc gatgtaacgg cgcgtcgccg
      541 cggcgaacag cggcgcgacg acaccgaggc cgaccgagcc ggtggagaag tcggccacgt
      601 ccgggtcctt ggtgcggctc gggtaggcct gcaacccacc gaaggaacgc agcgtggtga
      661 ggtacgaacg gtcgagttcg ccggtcaggt acttgatcgc atggaacacc ggcgacgcgt
      721 ggggcttgac cgccaccttg tcgttgcccg acagatgggc gaaccacagc gcggtcatga
      781 tcccgaccat cgatgccgac gacgcttggt ggccgccgac cttgacctcg tccggtttcc
      841 ggtcgtgatt ggcactgtcg acgatgcgca ccgccagcca caacagccgc tgctgaatct
      901 gctcaagaac gtcgatgtcc acgtcgtcga tgtcggtcat cggaacattg tgagtcacca
      961 gcatcgcgtg acacaacaga ccgctgccag cagctcggag aacgcaggat tccgccgcat
     1021 gagctccatt ttccatagaa tcactgcgtg agtgcagcga gtcacggcgg tgatctcgac
     1081 gagatcgacc gccacatcat cgacatcctc cgcgcggatg gacgggccac cttcggcgag
     1141 atcggtacca cggtcgggct gtcggcaccc gcggccaagc gccgcgtcga tcgcctggtc
     1201 gaccgcggcg tgatccgcgg cttcacggtg atggtcgacc acgccgcgct cggctttccg
     1261 ttggaagcct tcgccgaact gcgcttcgac ggtgacaccg cggtggagga gatcgacgcc
     1321 gtcgccgacg acattcccga ggtggtcggc atcttcaccc tcgccggcga tcccgatgcg
     1381 ctggcatggc tgcgcgtccg cgacgtgcac gacctgaagc gggtcatcaa cctgatccgc
     1441 aacaccggca aggtctccgg caccaagaca ctgatggtgc tcggcggatc ggacccgaag
     1501 ccctgaacgc tcagtagacg gggaacttgg ccttgtcggg cagaccggag cgggcggtga
     1561 tcgactcgcc catctgctgc gcgtcggccc acagcttggc ttcgtcgatc gtgatgcagt
     1621 ggccgtcttc aaccgtgacc tgcccgtcga ccaccacggt gtgcacaccg cgcccgtcgg
     1681 ccgaccacac caactggttg accacgttga gcagcgggcg ccactcgggc cgatcggcat
     1741 cgtgcagcac gacatcggcc ttcttgccga cctccaacga accgatctga tcggccatct
     1801 gcatcgtccg cgccccaccc atcgtcgcca tctcgtaggc aacctcggcc gggaacattt
     1861 gcgggtcggt gcgggcgtcc ttgaacaacc ccgcgacgag atacgtcgcc cgggccaggt
     1921 cgctgtagtt cgacgcgttg ttgccgtcgg tgccgatcga cacgttgatg ccgcgctgca
     1981 ccatctccgg catcttgccc acctgcgtca cgccgtagct gaccttcagc gcggtggtcg
     2041 ggcagtgagc gacgtgcgcg ccatgttcgg ccatcagcgc gatctcgctg tcgtccacgc
     2101 gcacgcagtg ggtcatcgcg acgttgctgt cgagcacccc gatctcggcc aggtgcacga
     2161 tcgggcgctg accgaactcg gccaagtacc actcgggatc gagcatcgcc ggcgacatgt
     2221 ggaacgacat gcccgtgccg tattcggcgg caagttgctt ggctgccttc cacagcgggt
     2281 cgctgatcgt gttgtgcccg accacgatgc tccacggccc gatccgccct gccccggtgt
     2341 cgcggtggtg ctccaactgg tgggcgagat ggccgatcgc ctcgtcggtc gtctggcgat
     2401 acaccgccgg ttccggcggt aggtcccaca cccagcggcc gaccttgccg cggatgccga
     2461 cttcgaccag cccgtcgatc acctcgtcga ggaagcggat cgtgcccgcc tccaggaacg
     2521 tggtggtgcc cgagcgcatc atctcgaccg cggcgagctg accggacgtg cgctcttcgg
     2581 ccgcgtcgaa caacgagtac agcggtgtca accactcgaa cacgttctcg acgaacggcg
     2641 tgtcgtcggg gacatagccg cgggtcaacg gctcgccggt gatgtggatg tgggtgttga
     2701 tcaacccggg ggtgacgacg aatcgatcgc cgccgatcgt gcggcgcggc gcgtagcggc
     2761 gggcgaggtc ggcggctttg ccgaccgcga cgatcgtgtt gccgtcgatc gcgaccgcac
     2821 cgtcacgaat gacgtcgcgg tgctcgttca tcgtcaccac gtaccagccg cggaccaaca
     2881 ggtcgatcgg ctgcggtgcg gctgggttgg tgtcgtgcgt gtccatgtgg gttggacctc
     2941 ctgccttgag tgccagtggt gatgatcggg tcagcgtttg gtggcgacgt agacgaggtt
     3001 gaagccgatc ggctccagca tccgcaccgc ggtgaagcct gcgtcggtca accacgacga
     3061 gacctgggcg taggtgagca ctcccccgcg ctcggnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn cacgacgtcg aacgcgccgt ccgcgatgcc gatggtgtgg aagtcaccgg
     3301 gcaggtactg cacccgtgtc gccaggtcgg cgccgtcggg ctgagctgcg atcgtgtccg
     3361 cggccagttc gatcacaccg ggcaggtcgt tgacgaccgc ggtcgaaccg ggcgactgct
     3421 gcagcaccgc gagcgcgtag ggggcgcggc ccgcgccc
//
LOCUS       DAORNF010000329         3454 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_20602_length_3454_cov_1.803177, whole genome shotgun sequence.
ACCESSION   DAORNF010000329 DAORNF010000000
VERSION     DAORNF010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3454
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20602_length_3454_cov_1.803177"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>566
                     /locus_tag="PLV68_12910"
     CDS             <1..>566
                     /locus_tag="PLV68_12910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02195.1"
                     /translation="LDVAGLYHHAERAYEWLVDIQDDAGWWNNYYLPDGSIEDVKLDT
                     NVCAYIATGVWHHWLCTWDRGFIDHLWPSVERAIDWVLSMRRPDGTVLWAVEPDERPW
                     DYALLTGCSSIQHSLDCAANLAELINEPRPDWAAAADTLRTVIAARPDTAFEPKTRWA
                     MDWYYPVLTGAMTGEQANGSRRETDCHA"
     assembly_gap    567..607
                     /estimated_length=41
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <608..907
                     /locus_tag="PLV68_12915"
     CDS             <608..907
                     /locus_tag="PLV68_12915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02196.1"
                     /translation="EPWVTASETAECSMAFRAIGDTDTATDLLIWTRAHRRDDGAYDT
                     GIVYPSLERFPTDEVSAYTGAAVIMAADSITGASAGSKLFVPSATTGTAGTADLD"
     assembly_gap    996..1005
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1006..>1628)
                     /locus_tag="PLV68_12920"
     CDS             complement(<1006..>1628)
                     /locus_tag="PLV68_12920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02197.1"
                     /translation="SKLPLIIGALVVIVAIVVVLVLVLAGDDDQQAAFDVTAAYDGLE
                     ALIDDADLSEGTQSVRNCPLGDIDDLVELIGKKVALSTEVVDGSPDHAFVDADGTTAA
                     FVYCQTFTDDDDIDGPTGVYVEALLDAPRRYESSVEDRYGDSTIIDFEKDTRPLSGGE
                     TYLFCAEERDETGFTGCEADWVHSEADITLIVFLGGEDTSVDEAYAA"
     assembly_gap    1629..1720
                     /estimated_length=92
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1863..>2090)
                     /locus_tag="PLV68_12925"
     CDS             complement(1863..>2090)
                     /locus_tag="PLV68_12925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRE02198.1"
                     /translation="FLFVDGGHGEQPARDDYHGWVRHVALGGTLAIHDVFPDPADGGR
                     PPYEQIYLPALESGLFELRSVTGSLRVLTRI"
     assembly_gap    2091..2138
                     /estimated_length=48
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2139..2504)
                     /locus_tag="PLV68_12930"
     CDS             complement(<2139..2504)
                     /locus_tag="PLV68_12930"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRE02199.1"
                     /translation="MRLPEPYRGHAIAAKGFMPPDEGDALYAAALEAGAACPGLAWVE
                     VGSYCGRSTIWLGAAALAVGTTLFAVDHHRGSEENQSGWEHHDPTVVDARIGRMDTLP
                     TFRATVYDAGVEDAVIAVVG"
     gene            complement(2514..>3069)
                     /locus_tag="PLV68_12935"
     CDS             complement(2514..>3069)
                     /locus_tag="PLV68_12935"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HRE02200.1"
                     /translation="RRVPFGEYMPLRGLLEALGAPTDLVPRNAVAGSDPAFLTLPDDT
                     RVGVAISWEIFFGGRGRDGIGHGGQLLINPTNGSSYTWTVLQTQQIASSRLRAIETGR
                     WVVQAAPTGFSAFVSPDGEVFDRTGVSEQAVITRTVALRSGDTWYLHIGDKPWVALAA
                     VLLALAWHRSGWRPSQLRHRRDER"
     assembly_gap    3070..3102
                     /estimated_length=33
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3103..>3454)
                     /locus_tag="PLV68_12940"
     CDS             complement(<3103..>3454)
                     /locus_tag="PLV68_12940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02201.1"
                     /translation="SLRIAAVQGGGPQGTHAVDYVSCRADGQIDPAGQFAGPRCVTLR
                     QLAATATLRPDDDIDVVVWPENVVDVDDVPFEQSVQFAEIAAEAERLGATFLVGVTED
                     TTDDRFTNAQMVVNP"
BASE COUNT          512 a         1128 c         1098 g          492 t
ORIGIN      
        1 ctcgacgtgg ccggcctgta ccaccatgcc gagcgggcct acgagtggct ggtcgacatc
       61 caagacgacg cgggctggtg gaacaactac tacctgcctg acggcagcat cgaagacgtc
      121 aagctcgaca ccaacgtctg cgcctacatc gccacaggcg tctggcacca ctggttgtgc
      181 acctgggacc gcggcttcat cgatcacctc tggccgtcgg tcgagcgcgc catcgactgg
      241 gtgctgtcga tgcgccgtcc tgacggcacg gtgctgtggg ccgtcgagcc cgacgaacgg
      301 ccgtgggact acgcgctgct caccgggtgc agcagcatcc agcactcgct cgactgcgcg
      361 gccaacctgg ccgagttgat caacgagcct cggcccgatt gggccgccgc ggcagacacg
      421 ctgcgcaccg tgatcgctgc gcggcccgac acagcgttcg aacccaagac gcgttgggcg
      481 atggactggt actaccccgt gctcaccggg gcgatgacgg gcgagcaggc caacggaagc
      541 aggcgcgaga cggattgcca cgcctannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      601 nnnnnnngag ccgtgggtga ccgcgtcgga gaccgccgag tgctcgatgg cgttccgggc
      661 gatcggcgat accgacaccg ctaccgatct gttgatctgg acgcgtgccc accgccgcga
      721 cgacggcgcg tacgacaccg gcatcgtcta cccctcgctg gaacggttcc cgaccgacga
      781 ggtgtcggcc tacaccgggg ccgcggtcat catggccgcc gactcgatca cgggcgcatc
      841 ggcgggaagc aagctgttcg ttccatctgc aacaaccgga acagccggta cggcggacct
      901 cgactgaggg gcgtcgcggc ggcggtccga actcagcgac cggtgtcggc cgcttcggcc
      961 aaggtgcgca acaccggggt cagtacctct tgcagnnnnn nnnnncgctg cgtaggcctc
     1021 gtcgacgctg gtgtcctcac cgccgaggaa cacgatcagc gtgatgtcgg cctcgctgtg
     1081 cacccagtcg gcctcgcagc cggtgaaccc ggtctcgtcg cgctcttcgg cacagaagag
     1141 gtaggtctcg ccaccgctca aggggcgggt gtccttctcg aagtcgatga tggtgctgtc
     1201 gccgtagcga tcttcgaccg aggactcgta ccggcgtggg gcgtcgagca gcgcctcgac
     1261 gtacacgccg gtcggcccgt cgatgtcgtc gtcgtcggtg aaggtctggc agtagacgaa
     1321 cgcggcggtc gttccgtccg catcgacgaa cgcgtggtcg gggctgccgt cgaccacctc
     1381 cgtgctgagc gcgaccttct tgccgatcag ttcgaccaga tcgtcgatgt cgccgagcgg
     1441 gcaattgcgc accgactggg tgccttcgct gaggtcggcg tcgtcgatga gtgcctccaa
     1501 cccgtcgtag gcggccgtga cgtcgaacgc cgcctgctgg tcgtcgtcgc ctgccaacac
     1561 cagcaccaac acgacgacga tcgccacgat cacgaccagc gcgccgatga tcaggggcag
     1621 tttcgactnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ggcgccgaac tggcgcccgt
     1741 caccgggcgg gggtccacca cccggcggca agaagttcgg gggagtgctc accccggcca
     1801 ctgtaggcgt cggcgccagg tcacccggag tactcgccgg tgccccctgt ggggatcgac
     1861 gctcagatgc gggtcagcac tcgcagcgag ccggtgaccg agcgcagttc gaacaggccg
     1921 ctctccaacg cgggcaggta gatctgctcg tacggtggac ggccgccgtc ggccgggtcg
     1981 gggaacacgt cgtggatcgc cagcgtgccg cccagggcga cgtggcgaac ccagccgtgg
     2041 tagtcgtcac gggcgggttg ttcgccatgg ccaccgtcga cgaagaggaa nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngc cgaccaccgc gatcaccgcg
     2161 tcttcgacgc ctgcgtcgta gacggtcgcc cggaacgtcg gcagcgtgtc catccgcccg
     2221 atccgggcat cgaccacggt cgggtcgtgg tgctcccaac ccgactggtt ctcctcggat
     2281 ccgcggtggt ggtcgacggc gaacaacgtc gtccccacag ccaacgccgc ggcccccaac
     2341 cagatcgtgg agcggccgca gtacgacccg acctcgaccc acgccaaccc gggacatgcc
     2401 gccccggcct ccagcgcggc ggcgtacagc gcgtccccct cgtcgggcgg catgaacccc
     2461 ttcgccgcga ttgcatgccc ccgatacggc tccggcaacc tcacccctgc accctaacgc
     2521 tcatctctcc gatggcggag ttgtgagggg cgccagccgg agcggtgcca ggcaagggcg
     2581 agcagcacgg cggcgagcgc gacccacggc ttgtcgccga tgtgcaggta ccaggtgtcg
     2641 ccgctgcgca gcgcgaccgt gcgggtgatc acggcttgtt cgctgacacc tgtgcggtcg
     2701 aacacctcac cgtcgggtga gacgaacgcg gaaaagccgg tcggagcagc ctgcaccacc
     2761 caccgcccgg tctcgatcgc gcgcaggcgc gacgacgcga tctgttgcgt ctggagcacc
     2821 gtccaggtgt agctgctgcc gttggtgggg ttgatcagca gttggccccc gtggccgatg
     2881 ccgtcacggc cgcggccgcc gaagaagatc tcccacgaga tcgccacccc aacacgagtg
     2941 tcgtcaggca gggtgagaaa cgccgggtcg cttccggcga cggcgttgcg gggtaccagg
     3001 tcggtgggcg cgcccaacgc ttccagcagg ccgcgcaggg gcatgtactc gccgaacggc
     3061 acccgccgcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncggggttg accaccatct
     3121 gggcgttggt gaagcggtcg tcggtggtgt cttcggtgac gccgaccagg aacgtcgcgc
     3181 ccaaccgctc ggcctcggcc gcgatctcgg cgaactgcac actctgctcg aacggtacgt
     3241 cgtcgacgtc gaccacgttc tccggccaga cgacgacgtc gatgtcgtcg tctgggcgca
     3301 acgtcgccgt cgccgccagt tggcgcagcg tgacgcagcg cgggccggcg aactggcccg
     3361 cggggtcgat ctggccgtct gcccggcacg acacgtagtc gaccgcgtgc gtgccctgcg
     3421 ggccgccgcc ttgcacggct gcgatgcgca gcga
//
LOCUS       DAORNF010000330         3450 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_20630_length_3450_cov_1.498969, whole genome shotgun sequence.
ACCESSION   DAORNF010000330 DAORNF010000000
VERSION     DAORNF010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3450
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20630_length_3450_cov_1.498969"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="PLV68_12945"
     CDS             <1..259
                     /locus_tag="PLV68_12945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02202.1"
                     /translation="HRIMTDAAQELAGLATAAIERLGPLPIGLVGGVFTSPIVTATVA
                     SVIGDTMPGRHPVEAAAALATLGQQAADDLGYHAPTERPTR"
     gene            complement(306..1187)
                     /locus_tag="PLV68_12950"
     CDS             complement(306..1187)
                     /locus_tag="PLV68_12950"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRE02203.1"
                     /translation="MARPPTTLERLRVFHAVVSAGTIAGAARSLDYTPSAVSQHLSAL
                     EREAGVALVERSNRGIVATDAGRLLAGSASDVLDQVHDVFDRVASVAGHHETTIAVAA
                     FPTAVTSLLLPLLDVLAPAIRLTIVDAEAETAMRALRGREVDGAITDGHASDPPLDDV
                     HRTLLRVEPIQLVTRSDRRVRHLADCAGAPWVLGGPRSRLAAAARQACAAAGFTPTVI
                     AESDDHHVTFDVIRATGAVSLLPELANGNVPDGITVARRVETGVDRRVEFVTRPPLGA
                     NPAIARLTQLLVARASR"
     gene            1259..>1571
                     /locus_tag="PLV68_12955"
     CDS             1259..>1571
                     /locus_tag="PLV68_12955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02204.1"
                     /translation="MSSPTLTTAAPHPPARAWLSTAAAAREAEVCATLDLTQFAAVDQ
                     RVHELVGRSRDIHDRECINLNPASNVMNPRAEAVLAAGLTTRASLGHPGDKYEMGLEA
                     IE"
     assembly_gap    1572..1683
                     /estimated_length=112
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1684..>2036
                     /locus_tag="PLV68_12960"
     CDS             <1684..>2036
                     /locus_tag="PLV68_12960"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF012677.2"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HRE02205.1"
                     /translation="CQPGDSIIVPPAEIGGHVTHRDAGAAGLYGLDIHECPADPDSFT
                     YDLAALAALAERVRPKLITIGTSLNLVPHPVDAIRDIADGVGARVLFDAAHACGMIAG
                     GVWPNPLDQGADLMT"
     assembly_gap    2037..2228
                     /estimated_length=192
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2229..>2561
                     /locus_tag="PLV68_12965"
     CDS             <2229..>2561
                     /locus_tag="PLV68_12965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02206.1"
                     /translation="IGFAGVALAAELEQRGLPVYRTRLGPTTSHQLAFDATRWGGGHA
                     TAQRLRVANLLACAIGLPGAPEGAGLRLGTPEIVRMGMTVADMTELASLIADALDGDP
                     AAVAPRTS"
     assembly_gap    2562..2602
                     /estimated_length=41
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2645..>3450
                     /locus_tag="PLV68_12970"
     CDS             2645..>3450
                     /locus_tag="PLV68_12970"
                     /inference="COORDINATES: protein motif:HMM:NF017532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HRE02207.1"
                     /translation="MSVDHTSRPTVSLTPPSADAAALTTEQLATLDRDGVIALPNRFS
                     ADEVAAINAALDELIGQDVPANIRERHSGVVRTAMGLHLRHPLFARLVCDPRFVVPGR
                     QVLGDVPVYAQQVKVNVKEAFTGEQWQWHYDFATHHHEDGVPEPLALNMHIFLDDVTE
                     VNGPLMFIRGSHADGPAPTALDTTTTSFPLWTVGPEVVAPLAERGGITCPTGPAGTML
                     LFGDAIVHGSPANMSPWSRRIFSLIVNPISNHQQSFARADHLHHRTTTPLT"
BASE COUNT          502 a         1162 c         1021 g          420 t
ORIGIN      
        1 ccaccggatc atgaccgacg ccgcgcagga gctcgccggc cttgcgaccg cggcgatcga
       61 acggctcggc ccactgccga tcggcctcgt cggcggcgtg ttcacctcac cgatcgtcac
      121 cgccactgtc gccagcgtga tcggtgacac gatgccgggc agacaccccg tcgaagccgc
      181 ggccgcactg gccacgctcg ggcagcaggc agcagacgac ctcggctacc acgcacccac
      241 cgaacgaccg acccgttgac cggcccacgt ccacgccgag ccgacggaag ctacagaaca
      301 gcggctcagc gtgaagctcg ggcgaccagc agttgggtca ggcgggcgat cgccgggttg
      361 gcacccaggg gcggccgggt gacgaactcg acgcggcgat cgacgcctgt ttcgacgcgg
      421 cgggcaaccg tgatcccgtc aggcacattg ccgttcgcga gctcgggcag gagcgacacg
      481 gcgcccgtcg cccggatgac gtcgaaggtg acgtgatggt cgtcgctctc ggcgatgacc
      541 gtgggcgtga acccggcagc cgcgcacgcc tggcgcgccg ccgcggcgag acggctgcgt
      601 gggccaccca gcacccacgg ggcaccggca cagtccgcaa gatggcgcac gcgccgatcg
      661 gaccgggtga ccaactggat cggctcgacg cgcagcagcg tgcggtgcac gtcgtcgagc
      721 ggggggtcgg atgcgtgtcc gtcggtgatc gcgccgtcga cctcgcgacc gcgcaatgcg
      781 cgcatcgccg tctcagcctc ggcgtccacg atcgtcaatc ggatcgcggg tgcgagcaca
      841 tcgagcagtg ggagcaagag cgacgtgacc gccgttggga aggctgccac cgcgatcgtc
      901 gtctcatggt gacccgcgac ggaagccact cggtcgaaca cgtcgtgcac ctggtcgagt
      961 acgtcggacg ccgaccctgc cagcagccgc ccggcgtcgg tggcgacgat gccccggttc
     1021 gaacgctcga ccaaggcgac ccccgcttca cgctcgagcg cactgagatg ttgcgacacg
     1081 gccgacggtg tgtagtcgag tgaccgcgcc gcacccgcga tcgtgcccgc cgagacgacg
     1141 gcgtggaaga cccgcagccg ctccagagtc gtcggtggtc gtgccatcag ttgagcttac
     1201 gactgtcgtc aggaatcatc gcttggcaag acctgtggaa cccgtcaagc tggcgggcgt
     1261 gtcatccccc actctcacca ctgctgcacc gcacccaccc gcacgggctt ggctcagcac
     1321 ggcggccgca gcgcgcgagg ccgaggtctg cgcgacgctc gacctgacgc agttcgccgc
     1381 ggtcgaccag cgcgtgcacg agttggtcgg gcgcagccgc gacattcacg atcgcgagtg
     1441 catcaacctc aacccggcgt cgaacgtgat gaacccgcgc gccgaggcgg tgctcgccgc
     1501 ggggctgacg acgcgggctt cgctcggcca tccgggcgac aagtacgaga tggggttgga
     1561 ggcgatcgaa gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnncctgcca accgggcgat tcgatcatcg tcccgcccgc ggagatcggc ggtcacgtca
     1741 cccatcgcga cgccggtgcg gcgggcctgt acgggctgga cattcacgag tgcccggccg
     1801 accccgactc gttcacctac gacctcgccg cgttggcggc actggccgag cgggtgcgcc
     1861 cgaagctgat caccatcggc accagcctca acctggtgcc acatcccgtc gacgcgatcc
     1921 gggacatcgc cgatggcgtc ggggcgcggg tcctgttcga cgccgctcac gcgtgcggca
     1981 tgatcgccgg tggcgtgtgg ccgaacccgc tcgatcaggg cgccgacctg atgacgnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnngc atcggcttcg ccggtgtcgc gctcgcggcc gaactggaac agcgcggcct
     2281 gccggtatac cgcacccggc tcggaccgac gacctcgcac caactcgcct tcgacgcgac
     2341 ccgctggggt ggcggccacg cgacggccca gcggctgcgc gtcgccaacc tgctcgcctg
     2401 cgcgatcggc ctgcccggtg cgcccgaggg cgcgggccta cgcctcggca cacccgagat
     2461 cgtgcggatg ggcatgaccg tggccgacat gaccgagttg gcgagcctga tcgccgacgc
     2521 gctcgacggc gatcccgcag cggtcgcgcc gcgcacgagc gnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnagtcgccc tacccgccac cacgcacccg agagcaacac
     2641 gaccatgtct gtcgaccaca catcccgccc cacggtcagc ctcacgccgc ccagcgctga
     2701 cgccgcggcg ctgaccaccg agcaactcgc cacgctcgat cgcgacggcg tgatcgcgct
     2761 gcccaaccgg ttcagcgccg acgaggtggc ggcgatcaac gccgcgctcg acgagttgat
     2821 cggccaggac gtgcccgcca acatccgcga acgccatagc ggtgtcgtcc gcacggcgat
     2881 gggcctgcac ctgcgccatc cgctgttcgc ccgactcgtg tgcgacccgc gcttcgtcgt
     2941 gccgggccgg caggtgctcg gcgatgtgcc ggtgtacgcg caacaggtga aggtcaacgt
     3001 caaggaggcc ttcacgggcg agcagtggca gtggcactac gacttcgcca cccaccacca
     3061 cgaagacggt gtgcccgaac cgctcgcgct gaacatgcac atcttcctgg acgacgtgac
     3121 cgaggtgaac ggaccgctga tgttcatccg cggttcgcac gccgacggtc ccgcaccgac
     3181 cgcgctcgac accaccacga ccagcttccc gctgtggacc gtcggcccag aggtcgtcgc
     3241 gcccttggcc gagcggggcg gcatcacctg ccccaccggc ccggcaggca cgatgctgtt
     3301 gttcggcgac gcgatcgtcc acggctcccc cgccaacatg tcaccctggt cacgccgcat
     3361 cttctcgctg atcgtcaacc cgatcagcaa ccaccagcag tcattcgccc gcgccgacca
     3421 cctccaccac cgcaccacca cccccctcac
//
LOCUS       DAORNF010000331         3439 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_20725_length_3439_cov_1.507092, whole genome shotgun sequence.
ACCESSION   DAORNF010000331 DAORNF010000000
VERSION     DAORNF010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3439
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20725_length_3439_cov_1.507092"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(46..>229)
                     /locus_tag="PLV68_12975"
     CDS             complement(46..>229)
                     /locus_tag="PLV68_12975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02208.1"
                     /translation="AGYGERESPWGRPATWCPRCQPAPDVRGGELLRVSADAERAARL
                     LALHPARREPAWTLGR"
     assembly_gap    230..379
                     /estimated_length=150
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<380..928)
                     /locus_tag="PLV68_12980"
     CDS             complement(<380..928)
                     /locus_tag="PLV68_12980"
                     /inference="COORDINATES: protein motif:HMM:NF013328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HRE02209.1"
                     /translation="MAEGDTIRRIADKVLARLVGQRCVGTTTRDPRLVGTDFTGRTLV
                     SADAVGKHLFLRFDDGRSLHAHLLMTGNIVVGPAAPVAPSRRRIEIDFEPGRFTGVDV
                     PLLDVIQTADEPSLIGHLGPDLCGPNPPDIVEITRRLAHDPAVPLAEAMLDQRNVAGF
                     GNVYAVEVPFIVGISPLQPAGTI"
     gene            complement(992..1654)
                     /locus_tag="PLV68_12985"
     CDS             complement(992..1654)
                     /locus_tag="PLV68_12985"
                     /inference="COORDINATES: protein motif:HMM:NF019301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HRE02210.1"
                     /translation="MFGPRSLTRPLLAAPFVLGGLAALRSPRSGNAQVVASIPAGTRN
                     AQLTGAHPDGTPVAQPPTSETQPNETQHGEAQHAATPQAEGLIDDIAEAVGLPGNSAM
                     LIRINGAVQVGAGVLFALGIVPRPAAVVLAATLVPSTAAGHPFWDGSEPASRTIGQFA
                     KNAGLLGGLLFAALDTGGRPSVFWRGRQVAGCAANSISGAAQSVADSVANAYHSLPIV
                     ND"
     gene            1823..>2246
                     /locus_tag="PLV68_12990"
     CDS             1823..>2246
                     /locus_tag="PLV68_12990"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRE02211.1"
                     /translation="MPGPMEGVKVVELGVWVAAPAACGVLADWGADVVKIEPPAGDPA
                     RTFRRMLGGDMPTNPVFELDNRGKRSIVIDLNTEAGRAIGMELLADADVFVTNVRADG
                     LARLGLDPPTLLAHNPRLVYGHISGYGLEGSDANRAAYD"
     assembly_gap    2247..2280
                     /estimated_length=34
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2281..3032
                     /locus_tag="PLV68_12995"
     CDS             <2281..3032
                     /locus_tag="PLV68_12995"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRE02212.1"
                     /translation="GGDPPFQRGGMGDHPTGMTMAGAISAALFARERTGHGQLVSTSL
                     FRQGVYTIGFDLNLALGWGVHPAIGERETMANPCINNYVSGDGHRFWVVGLEGDRHWP
                     PLARVVGHPEWLTDERFDSLRNRARNAVELVRLLDEAFATKTMAEWEAIFANEPDMFW
                     APVQSPDEVLNDPQLRDAGGLVEVPDEYGTTTMIASPADFDGTPWTPRWIAPKLGEHT
                     AAVLSELGRDAAAIDALVASGAVALESDEAL"
     gene            3242..>3439
                     /locus_tag="PLV68_13000"
     CDS             3242..>3439
                     /locus_tag="PLV68_13000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02213.1"
                     /translation="MIASAFGTVLVAVGIVGLGLNARHLGVVAVGLLAVVGALWLLVR
                     CARVGIVIDRSGIVVRNVTNTR"
BASE COUNT          491 a         1123 c         1153 g          488 t
ORIGIN      
        1 tgctcgccat gatccttgac ggaaggacct cgccgcggcg cgccctcagc ggccaagggt
       61 ccaggccggc tcacgccggg ccgggtgcag ggcgagcaag cgggcggccc gctcggcatc
      121 ggcgctgacg cgcagcaact caccgccgcg tacatccggg gcgggctgac agcgtgggca
      181 ccacgtcgct ggccgacccc acgggctctc ccgctcgcca tagccggccn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnng atcgtgccgg ccggttgcag cgggctgatc cccacgatga
      421 acggcacctc gaccgcatag acgttgccga agcccgccac gttgcgttgg tcgagcatcg
      481 cctccgccaa cggcaccgcc gggtcgtggg ccagacgacg ggtgatctcg acgatgtccg
      541 gcggattcgg gccgcacaga tcgggtccga gatgaccgat caacgacggt tcgtccgcgg
      601 tctggatcac atcgagcaga ggcacgtcga cgccggtgaa ccggccgggc tcgaagtcga
      661 tctcgatccg ccgccgcgat ggcgccaccg gcgctgcagg gccgaccacg atgttgccgg
      721 tcatcagcag gtgggcatgc agcgaccggc cgtcgtcgaa gcggaggaag aggtgcttgc
      781 cgaccgcgtc ggcactgacc aaggtgcggc cggtgaagtc ggtgccgacg agccgcgggt
      841 cgcgcgtcgt cgtgccgacg cagcgctgtc ccaccaggcg cgccaagacc ttgtcggcga
      901 tgcggcggat cgtgtcacct tcggccacgt cgccagcctg gcacgactcc cgcggtgagc
      961 aatacgtcga tcgccgtgcc ctgtgtcatg ctcagtcgtt gacgatcggc agcgagtggt
     1021 aggcgttggc gaccgagtcg gccaccgact gcgccgcccc ggagatcgag ttcgcggcac
     1081 atccggccac ctggcggcct cgccagaaca ccgacgggcg gccaccggtg tccaacgccg
     1141 cgaacagcag cccaccgagc agaccggcgt tcttggcgaa ctgtccgatg gtgcggctcg
     1201 ccggttcgct gccgtcccag aacgggtgac cggcagccgt cgagggcacc agggtcgcgg
     1261 ccagcacgac ggctgccgga cggggcacga tgcccaaggc gaacagcacc ccagcgccta
     1321 cctgcaccgc gccgttgatg cggatcagca tggccgagtt gcccggtaac ccgacagctt
     1381 cggcgatgtc gtcgatcaag ccttcggcct gcggcgtcgc ggcgtgctgt gcctcaccgt
     1441 gctgtgtctc attaggctgt gtttcactgg tcggtggctg ggcgaccggt gttccgtcag
     1501 gatgggcgcc ggtgagttgg gcattgcggg tgcccgccgg gatcgatgcg acgacttggg
     1561 cgttgccgct ccggggcgag cgcagcgcgg cgagaccgcc gagcacgaac ggcgcagcga
     1621 gcagcgggcg ggtgagcgag cgaggtccga acatggacac tcacataccc acctccggcc
     1681 gcgatgaacc gtcacgcacg tcggccgcgc cgctctggtc gtcgcgaccc ggatcgcctc
     1741 ggtactgtcc ggctggtccg cgggcgctct cgcgacgcaa caggacgacg agatcgaacg
     1801 agccagaggg aaggagacgc tgatgccggg gccgatggag ggtgtcaagg tcgtcgagtt
     1861 gggcgtgtgg gtcgccgcgc ccgcggcctg tggggtgctg gccgactggg gtgcggacgt
     1921 cgtcaagatc gagccacccg ctggcgaccc ggcgcgcacg ttccggcgga tgctcggcgg
     1981 cgacatgccg accaaccccg tgttcgagtt ggacaaccgc ggcaagcgca gcatcgtgat
     2041 cgacctgaac accgaggccg gacgggcgat cggcatggag ttgttggccg acgccgacgt
     2101 gttcgtcacc aacgtccgtg ccgatgggtt ggcccgcctc ggtctcgacc caccaacgct
     2161 gctggctcac aacccgcggc tggtctacgg ccacatcagc gggtacggcc tggagggctc
     2221 cgatgccaac cgagccgcgt acgacannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 cgggcggcga cccaccgttc cagcgtggcg gcatgggcga tcaccccacc ggcatgacga
     2341 tggccggcgc gatctcggcc gcgctgttcg cccgcgagcg cacgggtcac ggtcaactcg
     2401 tgtcgacgtc actgttccgc cagggggtct acacgatcgg cttcgatctc aacctcgccc
     2461 tgggctgggg tgtgcatccg gccatcggcg agcgggagac catggccaac ccctgcatca
     2521 acaactacgt ctcgggcgac ggtcaccgct tctgggtggt cggtttggag ggcgaccgcc
     2581 actggccgcc gctggcccgc gtggtggggc atcccgagtg gctgacggac gagcggttcg
     2641 actcgctgcg caaccgggcc cgcaacgcgg tcgagctcgt ccggctgctc gacgaagcct
     2701 tcgctaccaa gacgatggcc gagtgggagg caatcttcgc gaacgagccg gacatgttct
     2761 gggcgccggt gcagtcaccc gacgaggtgc tcaacgaccc gcagttgcgc gacgccggcg
     2821 gtctggtcga ggtgcccgac gagtacggca cgacgacgat gatcgcatcg ccagccgact
     2881 tcgacggcac accgtggacg ccgcggtgga tcgcccccaa gctcggcgag cacaccgccg
     2941 cggtcctttc cgaactcggc cgcgatgccg cagcgatcga cgcgctggtc gcctcgggcg
     3001 cggtcgcgct ggagagcgac gaggccctct gacgtcgccc accgaggacc cgctgaggcc
     3061 gccgccaccc cccgatctcg tgtcgagttg gtggtcgaac acagccgaag catcgagcac
     3121 ggaggtttga cgcaaccccc agctttcgtc acgactgctg gtaccttgcg gcgccgtgtc
     3181 acgacagcaa tccgagtcag gtccccggtt ccggttccgc gtcgctgcga tgggactggc
     3241 gatgatcgcg tccgcgttcg gcaccgtgct cgtggcggtc ggcatcgtcg ggctggggct
     3301 caacgcccgt catctcggcg tggtcgcggt cggcctgctt gccgtggtcg gcgcgctgtg
     3361 gctcctggtt cgctgcgcgc gggtcggcat cgtgatcgac cgcagcggca tcgtcgtgcg
     3421 caacgtcacc aacacgcgg
//
LOCUS       DAORNF010000332         3428 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_20802_length_3428_cov_2.039431, whole genome shotgun sequence.
ACCESSION   DAORNF010000332 DAORNF010000000
VERSION     DAORNF010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3428
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20802_length_3428_cov_2.039431"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            78..>3428
                     /locus_tag="PLV68_13005"
     CDS             78..>3428
                     /locus_tag="PLV68_13005"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018262 - isopeptide cross-linking
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HRE02214.1"
                     /translation="MLDKKVGTAATSANCDTAAWVDADEPLAAAALFRPGDRVCYLVT
                     LSLFGSDAGSDLLYRNATVTDFLPPYMAFEQFWGSDEVGSTLASSVVEVSATVCDDLT
                     DPNPCDPDVGTQITWTLGQGGDDDKYVDPGSSTTPEKTFQVVFSARYSALPSAVGTGV
                     FDAEANLVKATTFNTAGQATSLRDAGVHVATRPALSLDKSRTPSTATVPAGSAITYTV
                     AVTNDAPLEPGNPGEKMGAAYDVAVRDVLPREITCANVSAISNGGTCADGWVISGVSY
                     SSITWSIAGPVNPGDTVNRTYVVTLHDALAPNEGLDNTAGVTRYESHTNVSTDPSVVY
                     VPPTAPQVDPAAVPNDTVPALDRETVRIVQATPVKLQQSSVDESNNGANASLSNANDF
                     ATIGELVTYRVSVDVPAHSTVYNARLTDSLPTGLVVVGTPTFSFNGGALPVDFTTSNT
                     PPTITFPTVYQNATSAAQTFQIDFQARVTTLGTNTHNTNRTNVAHFTFDTFADHNEWA
                     TEPQDTTSSTIVQIKEPNPSISKSSSPVENVLPGQTVTYTVVASNASGRSPAHDLVVT
                     DCIPAGLTYTTGSATTPNGSTLTVASGATGCLTGTLLTWTYPTAYAINANASTTMTYA
                     VTVDTPAVTGTIFTNTARLSANSLTGPVGEVRNYTDSTTDTVRVVNPVVTKTHTPDPV
                     RVGEDAAYTLQFTLPANQRMYDSRILDEIPTGMRYTGLVSFSCSPTVDCPGMTVLGGP
                     SFGNGDDVTFWFGDVAPHTTDRVLTVVYTAIVMDLSANVSGNTFVNTARLTWNQTDTV
                     TGTPSTPPSTPGGTPATPVNDTTNLVEPKLTIDKDVTLVGCDNTAFGTGANDDDVCSY
                     APTGAALQYTVRVRNTGTSAAYDITVTDDVDLLDTAFSAVTLGANPGWTVVDSDITDG
                     DGLELTYDGPLAADATIEFTYTATLKPSADLSQLQHVLNTAAIPTYYGLPNRDPNERT
                     YSENPQDQVDVTLHYPQPSIVKTAVSDATDARAGQPFTWQVVVANTDTVATLFNYDLA
                     DNLPPGWTYVASSATVTSVTSGGTPGVGAIAAAPTITGDGTIAEPQTMTWTNLGNLPP
                     NATVTIRYQATPSAALETLATTGAHDHTN"
BASE COUNT          629 a         1305 c         1022 g          472 t
ORIGIN      
        1 gcgctatctg agcttcagcc agcaggacat catccgcggt gccgagatcg gcgttcgggc
       61 agagcgcggc gaccggcatg ctcgacaaga aggtcggcac cgcggcgacc agcgccaact
      121 gtgacaccgc ggcgtgggtc gacgcggacg aaccgttggc cgcggccgcg ctattccgcc
      181 ccggcgaccg ggtgtgctac ctggtgacgc tgtcgctgtt cggcagcgat gccgggtccg
      241 acctgctcta ccgcaacgcg acggtcaccg acttcctgcc gccgtacatg gcgttcgagc
      301 agttctgggg cagcgacgag gtcggttcga cactggcgag ttccgtcgtc gaggtctccg
      361 cgaccgtgtg tgatgacctg accgacccca acccctgcga ccccgatgtc ggcacccaga
      421 tcacctggac gctcggccag ggcggggacg acgacaagta cgtcgatccg ggcagcagca
      481 ccacccccga aaagacgttc caggtcgtgt tctcggcccg ttactcggcc ttgccctcgg
      541 cggtcggcac gggcgttttc gatgccgagg ccaacctggt caaggccacg acgttcaaca
      601 ccgctgggca ggccacctcg ctgcgcgacg cgggcgtgca cgtggccacc cggcctgcgc
      661 tgtcactgga caagagccgc acccccagca cggccaccgt gcccgccggt tcggcgatca
      721 cctacaccgt cgcggtcacc aacgacgccc cgctcgaacc cggcaatccc ggtgagaaga
      781 tgggcgcggc ctacgacgtc gcggtgcgcg acgtgttgcc ccgcgagatc acctgcgcca
      841 acgtcagcgc catcagcaac ggcggcacgt gcgccgacgg ttgggtgatc agcggtgtgt
      901 cgtactcgtc gatcacgtgg tcgatcgccg ggccggtcaa ccccggcgac acggtcaacc
      961 gcacctacgt cgtcaccctc cacgacgcgc tcgcccccaa cgagggcctc gacaacacgg
     1021 ccggcgtgac ccgctacgag agccacacca acgtgtcgac cgacccgtcg gtggtctacg
     1081 tgccgccgac tgcgccccag gtcgacccgg ccgcagtgcc caacgacacg gtgcccgcgc
     1141 tcgaccgtga gacggtgcgg atcgtgcagg ccactccggt caaactgcag cagtcgtcgg
     1201 tcgacgaatc caacaacggc gccaacgcct cgttgtccaa cgcgaacgac ttcgccacga
     1261 tcggcgagtt ggtcacctat cgcgtttccg tcgacgtgcc ggcgcattcg acggtgtaca
     1321 acgcccggct caccgactcg ctccccacgg gcctggtggt cgtgggcacc ccgacgttca
     1381 gcttcaacgg cggcgcgctg ccggtggact tcaccacgtc gaacacgccg ccgacgatca
     1441 ccttccccac cgtgtaccag aacgcgacct cggccgcgca gacgttccag atcgacttcc
     1501 aggcccgtgt caccacgctg ggcaccaaca cccacaacac gaaccgcacc aacgtggctc
     1561 acttcacgtt cgacaccttc gccgatcaca acgaatgggc caccgaaccg caggacacca
     1621 cgtcgagcac gatcgtccag atcaaggaac cgaacccgtc gatctccaag tcgagcagcc
     1681 cggtcgagaa cgtgctgccc ggccagaccg tgacctacac ggtggtcgcc tccaacgcca
     1741 gcggccgctc gcccgcccac gacctggtcg tcaccgactg catcccggcg ggactgacct
     1801 acacgaccgg ttcggccacc actccgaacg gttcgacgct gaccgtcgca tcgggcgcga
     1861 ccggatgtct caccggcacg ttgctgacgt ggacgtaccc caccgcgtac gcgatcaacg
     1921 ccaacgcatc cacgacgatg acctacgccg tcaccgtcga cacccccgcg gtcacgggca
     1981 cgatcttcac caacaccgcc cggctcagtg ccaactcact gaccggcccg gtgggcgagg
     2041 tgcgcaacta cacggacagc accaccgaca ccgtgcgcgt cgtcaacccg gtggtcacca
     2101 agacccacac gcccgacccg gtgcgcgtcg gcgaggacgc cgcgtacacg ttgcagttca
     2161 cgctgccggc caaccagcgg atgtacgact cgcgcatcct ggacgagatc ccgacgggca
     2221 tgcgctacac gggcctggtg tcgttctcct gctcaccgac cgtcgactgc ccaggcatga
     2281 ccgtgctcgg tgggccgagc ttcggcaacg gcgacgacgt gacgttctgg ttcggtgacg
     2341 tcgccccgca cacgaccgac cgcgtgctga ccgtcgtcta caccgcgatc gtgatggacc
     2401 tgtccgccaa cgtcagcggc aacacgttcg tcaacaccgc ccgcctgacg tggaaccaga
     2461 ccgacaccgt gaccggcacg ccgtccaccc cgccatccac gccgggtggc acgccggcga
     2521 ccccggtcaa cgacaccacc aacctggtcg agccgaagct gaccatcgac aaggacgtca
     2581 ccctcgtcgg ctgcgacaac accgcgttcg gcaccggcgc caacgacgac gatgtgtgct
     2641 cgtacgcgcc gaccggtgcg gcgctgcaat acacggtgcg ggtgcgcaac accggcacct
     2701 cggccgccta cgacatcacc gtcaccgacg acgtcgacct gctcgacacg gcgttctccg
     2761 ccgtcacctt gggagccaac cccgggtgga ccgtggtcga cagcgacatc accgacggcg
     2821 acggcttgga gttgacctac gacggcccgc tcgccgccga cgccacgatc gagttcacct
     2881 acaccgcgac gttgaagccg tcggccgacc tcagccagtt gcaacacgtg ctcaacacgg
     2941 ctgcgattcc cacctactac ggcctgccca accgtgatcc aaacgagcgg acctacagcg
     3001 agaacccgca ggatcaggtg gatgtgacgc tgcactaccc gcagccgagc atcgtcaaga
     3061 ccgcagtcag cgacgcgacc gacgcccgcg ccgggcagcc gttcacgtgg caggtcgtgg
     3121 tcgccaacac cgacacggtc gcgacgctgt tcaactacga cctggccgac aacctgccgc
     3181 ccggctggac ctacgtggcc agttcggcaa cggtcacgtc ggtgaccagc ggcggcacgc
     3241 cgggtgtcgg cgcgatcgcc gcggctccca ccatcaccgg tgacggcacg atcgccgaac
     3301 cgcagacgat gacgtggacc aacctcggca acctgccgcc gaacgccacc gtcacgatcc
     3361 gctaccaggc gacaccgagc gcggcgttgg agacgctggc gacgacgggt gcccacgatc
     3421 acaccaac
//
LOCUS       DAORNF010000333         3416 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_20884_length_3416_cov_1.479024, whole genome shotgun sequence.
ACCESSION   DAORNF010000333 DAORNF010000000
VERSION     DAORNF010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3416
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20884_length_3416_cov_1.479024"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>273)
                     /locus_tag="PLV68_13010"
     CDS             complement(<1..>273)
                     /locus_tag="PLV68_13010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002514361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRE02215.1"
                     /translation="TVSSGEVHAVMGPNGAGKSTLSAVVMGKPGYQVTEGSVTLDGVD
                     MLALPTWQRAQAGLHLVMQYPTEVPGVSIVDAMTEAVAHLRSHGPAV"
     assembly_gap    274..389
                     /estimated_length=116
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<390..724)
                     /locus_tag="PLV68_13015"
     CDS             complement(<390..724)
                     /locus_tag="PLV68_13015"
                     /inference="COORDINATES: protein motif:HMM:NF012575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HRE02216.1"
                     /translation="MVVAALDEMEPGSARKFDIGGRLVAVVRIADSVYAIGDTCSHGN
                     VSLSEGEVLSDELELECWKHGSTFSLLTGEPTTLPATQPVPVYVATVVDGDKLQYYDF
                     AKSYEEAQRL"
     assembly_gap    758..803
                     /estimated_length=46
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<804..>1749)
                     /gene="sufD"
                     /locus_tag="PLV68_13020"
     CDS             complement(<804..>1749)
                     /gene="sufD"
                     /locus_tag="PLV68_13020"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HRE02217.1"
                     /translation="RYRPGLAATVVEAPDAGVTIGAHAGDDAAFDAELDLFAELNRAF
                     CASTHVAIAAGAVVDEPIVVTHTITESGVVCFPRLVIDAGADSEATVIEVFRSDTDID
                     SLVLPVLRVRADQAARITYLAINDLGDGTWQIANQQSVGERDSTTVLATVALGGDYAR
                     ARTHARLVGKGATTRQVALYFADGTQTLDFRVTQEHVAPKTTSDLLFKGAVNDRSHSV
                     YTGLIKIGPDARGTQAFQTNRNLTLSEGAWAESVPNLEIDTNDVQCSHASTVGPIDEE
                     QRFYLESRGIHPDVAERLVVLGFFDEVLDRLPVGGHADV"
     assembly_gap    1750..1781
                     /estimated_length=32
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1983..2336)
                     /locus_tag="PLV68_13025"
     CDS             complement(1983..2336)
                     /locus_tag="PLV68_13025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02218.1"
                     /translation="MPMRQECKHFESRTYANGDTVRKCNLDLAPDAPWRCPDPCPRFE
                     RRLADVAWVHGSLITPPSPPEPPGLDDGTAAAVLDEAEDWLNEVGRQVRAEIEAGQPL
                     LPREGRFRNIFRRKK"
     gene            2476..>2911
                     /locus_tag="PLV68_13030"
     CDS             2476..>2911
                     /locus_tag="PLV68_13030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02219.1"
                     /translation="MDIVTVRAPRAGFVTRLDVREGQLVQRQAQLQAGRTPPRCGEQA
                     QTARGHHHQPDPAAVEHRVAALGAHGDRHEHDAEHQCDAELDRDQAQERLAAQAQHAG
                     QHGHREHRRHHADQHPPVVAHLVHPSHRERDLAAARRGEGAVG"
     assembly_gap    2912..3004
                     /estimated_length=93
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3005..>3416)
                     /locus_tag="PLV68_13035"
     CDS             complement(<3005..>3416)
                     /locus_tag="PLV68_13035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HRE02220.1"
                     /translation="AYAGAGQHQDAGAKMVHAAPETTSKIVSKSISKDAGITTYRGLV
                     RVEEGAYGCKSHVQCDALILDDHSKSNTLPYMEVGERDAQVGHEATVSKIADEQLFYL
                     MSRGLSQEQAMSMVVNGFIEPVTRTLPMEYAVEWS"
BASE COUNT          469 a         1078 c         1062 g          520 t
ORIGIN      
        1 acggcggggc catggctgcg cagatgggca accgcctcgg tcatcgcatc gacgatcgac
       61 acaccgggca cctcggtggg gtactgcatc accaggtgca gcccggcctg ggctcgctgc
      121 caggtcggca aggcgagcat gtccacgccg tcgagcgtga cgctgccctc ggtcacctgg
      181 tagccgggct tgcccatcac cactgcggac aacgtgctct tccccgcgcc gttggggccc
      241 atcacggcgt gcacctcacc cgacgacacc gtgnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnna gtcgctgggc ctcttcgtag gacttggcga
      421 agtcgtagta ctgcagcttg tcgccatcga ccaccgtcgc cacgtacacc gggacgggtt
      481 gtgtggcggg caatgtggtc ggctcgccgg tcagcaggct gaacgtgctg ccgtgcttcc
      541 agcattcgag ctcgagctcg tcagacagca cttcgccctc gctgagcgag acgttgccgt
      601 ggctgcacgt gtcgccgatg gcgtagaccg aatcggcaat gcgtacgact gccaccagac
      661 gaccgccgat gtcgaacttg cgcgccgagc ccggctccat ctcgtcgagc gcagcgacca
      721 ccaccccgct cattgtccac ctcgcccgtg tccaggcnnn nnnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn nnnacgtcgg catgacctcc gaccgggagt cgatcgagca
      841 cctcgtcgaa gaagccgagt acgacgagtc gttcggcgac atcaggatgg atgccccggc
      901 tttcgagata gaagcgctgc tcttcgtcga tcggcccgac cgtgctggcg tggctgcact
      961 gcacgtcgtt ggtgtcgatc tccaggttgg gcacgctctc ggcccacgcg ccctcgctga
     1021 gggtgaggtt gcggttggtt tgaaaggcct gcgtgccgcg cgcatcgggc ccgatcttga
     1081 tcaggccggt gtacacgctg tgcgagcgat cattgaccgc gcccttgaac agcaggtcgc
     1141 tggtggtctt cggggcgacg tgttcttgcg tgacgcggaa gtcgagcgtc tgggtgccgt
     1201 ccgcgaagta cagcgcgacc tggcgggtgg tcgcgccctt gccgaccaag cgggcgtggg
     1261 tgcgggcacg ggcgtagtcg ccgccgaggg cgacggtggc aagcaccgtg gtggagtcac
     1321 gctcgcccac cgactgctgg ttggcgatct gccaggtgcc gtcgccgagg tcgttgatcg
     1381 ccaggtacgt gatgcgcgcc gcctgatcgg cgcgtacgcg cagcaccggc agtaccagcg
     1441 agtcgatgtc cgtgtcgctg cggaagacct cgatcaccgt ggcttcgctg tcggcaccgg
     1501 cgtcgatcac caggcggggg aagcagacga ccccactctc ggtgatggtg tgggtcacca
     1561 cgatcggctc atcgaccacg gcaccggccg cgatcgcgac atgggtcgat gcgcagaacg
     1621 cgcggttcaa ctcggcaaag aggtcgagtt cggcatcgaa ggcagcgtcg tcaccggcgt
     1681 gagcgccgat cgtgacaccc gcgtcgggtg cctcgaccac cgtcgcggcc agccccggtc
     1741 gatatcgatn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncagatctcc tgctcgggcg
     1801 tgggcgtctc agcggctcgg aagcgttcga gagcgccaac gcggacgtcg gcgtcggcct
     1861 ggtgcaggct gcgcaccgcg tcgacggaca aggcgtcgtc ggtcagcaat ggcacgccct
     1921 ccaggctaac gccaccccct ggcgaaccag cctgggacgg gaccaaagac ccgggtcccg
     1981 ccctacttct tgcggcgaaa gatgttgcgg aagcggcctt cgcgcggcaa cagcggttga
     2041 ccggcttcga tctcggcccg cacctggcgg ccgacctcgt tgagccagtc ctcggcttcg
     2101 tcgagcactg ccgcggccgt tccgtcgtcg aggccgggtg gttcgggcgg gctcggcggc
     2161 gtgatcagcg agccgtgcac ccaagccaca tcggccaggc gccgttcgaa ccgcgggcac
     2221 gggtcggggc agcgccacgg cgcgtcggga gcgaggtcga ggttgcactt gcgcaccgtg
     2281 tcgccgttgg cataggtgcg gctctcgaag tgcttgcact cctgtcgcat cggcatcccc
     2341 acagtgtgcc agccgcgtca ccgcgcgtcg gcggcgtcct ccagttgctg gatcgcgcgg
     2401 cgcaggtgca gcacgtcgcg gcaagacggc accaaggtcg atctgacgct ggcgctcgac
     2461 gccaagcgcc gcacggtgga tatcgtcacg gtgcgcgcgc cgcgcgcggg cttcgtcacg
     2521 cggctcgacg tgcgcgaagg ccaactcgtc cagcgtcagg cccagttgca ggccgggcgc
     2581 accccgccac ggtgtggcga acaggcgcag accgcccgcg gtcaccacca ccaacccgac
     2641 ccggccgccg tcgagcatcg ggtcgccgcg ctgggtgcgc acggcgaccg ccacgaacac
     2701 gatgccgagc accagtgcga tgccgaactg gatcgcgatc aggcgcagga acgactcgct
     2761 gctcaggccc aacacgcggg tcagcatggt catcgcgagc accgccgcca tcacgccgat
     2821 caacacccgc cggttgtggc ccaccttgta cacccgagcc accgcgagcg ggatctcgcg
     2881 gctgcccgcc gcggcgaggg cgcggttggc cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnggctcc actcgaccgc gtactccatc ggcaacgtcc gggtgaccgg ttcgatgaac
     3061 ccgttgacca ccatgctcat cgcctgctcc tggctgagcc cacggctcat caggtagaag
     3121 agctgctcgt cggcgatctt cgacaccgtg gcctcgtggc cgacctgcgc atcgcgctcg
     3181 ccgacttcca tgtacggcaa cgtgttgctc ttggagtggt cgtcgaggat cagtgcgtcg
     3241 cactgcacgt ggctcttgca gccgtaggcg ccttcttcga cccgcaccag gcctcggtag
     3301 gtggtgatgc cggcgtcctt gctgatgctc ttggacacga tcttgctggt ggtctcgggg
     3361 gcggcgtgca ccatcttggc tcccgcgtcc tggtgctgac cggcccccgc gtaggc
//
LOCUS       DAORNF010000334         3409 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_20934_length_3409_cov_1.680978, whole genome shotgun sequence.
ACCESSION   DAORNF010000334 DAORNF010000000
VERSION     DAORNF010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3409
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20934_length_3409_cov_1.680978"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="PLV68_13040"
     CDS             complement(<1..182)
                     /locus_tag="PLV68_13040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02221.1"
                     /translation="MAERSVAPYLRADVHTLGADHAATERRVAAIEVQLAEMAAVVAG
                     LAEHQPAVLNAISSSNG"
     assembly_gap    434..443
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <444..1586
                     /locus_tag="PLV68_13045"
     CDS             <444..1586
                     /locus_tag="PLV68_13045"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRE02222.1"
                     /translation="VQHTTTPQLDQPGDSASSIEAPRHGTSDYFHHANHELRTLELER
                     VPKGATRALSVGAQGRWYFEWFERSVGPLDEHIGVEAYEDKPTDLPDYVTWVADTADH
                     MVGVADGSVDLVFAGQTSEHLWARELSGFLTEAHRVLRSGGVLALDSPNRLVTQHLLW
                     SHGGHTIELSAAEMKELLNLAGFEVLSVTGIWNCVIDGRIVALEYGIDNPAIFARRAA
                     AGTGRPDDSFVWWINARRSDRTIDDVTLRARVDQFFEAHWNTRVSRGLAPDCLNLNGP
                     LDIVPGTLSETLPFPLHQGTWTVSLRLAVGTWNDVSGFGVRIVAPRDHLIHDLPLSAA
                     TVTDSEATWTIEQPYLLFALTLQIRADDVSGPVSIRLPIEVAFQSG"
     gene            complement(1587..2375)
                     /locus_tag="PLV68_13050"
     CDS             complement(1587..2375)
                     /locus_tag="PLV68_13050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRE02223.1"
                     /translation="MPSAPESAVVGSAPLPDQLPDQVPDQSPAQLSVVMPAYNEQATI
                     AEIIGAVLASPYTAELIVVDDSSTDDTAAIVEAISDPRVKLIRQSPNQGKGAALRRGF
                     AAATQPFVIIQDADLEYDPADYVHMMQPLLDGRADVVFGSRFLTTHAHRVLYFWHSVG
                     NRFLTTLSNVFTNLNLTDMESCYKAFRREVLQSFELQEQRFGIEPELAAKVARGNWRI
                     YEVGISYSARTYAEGKKIGWRDGLRAIFCILRYSPLSTRFRSSH"
     gene            complement(2480..>3154)
                     /locus_tag="PLV68_13055"
     CDS             complement(2480..>3154)
                     /locus_tag="PLV68_13055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02224.1"
                     /translation="QAAQREIADLTEQLDSIQDPDVDLRQQLNDAQAKIDELTGQVGQ
                     LTTDLGNERAGRAADAEAAQQSQAAAVAEVQGQLNAVQSDLDAIRNLFPMDEDSFRAA
                     APLGDYTVTVERAQCTIINCLELSSLSLSFPDSTQVSGNRANGVLAFADGSYAARGQL
                     SSEQAPLCNGVETEATFLLVFHATTATASNGLLAATAMEGTYRETIDNGDCVGQFREY
                     KVAMTK"
     assembly_gap    3155..3169
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3170..3397)
                     /locus_tag="PLV68_13060"
     CDS             complement(<3170..3397)
                     /locus_tag="PLV68_13060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HRE02225.1"
                     /translation="MTVPTSPSPAQRPCPRCGERVGPDDHFCFSCGASLIAAETRPAP
                     RPNTARRVVAIIAGCLAAAAVSGLVVAYLDAR"
BASE COUNT          616 a         1074 c         1091 g          603 t
ORIGIN      
        1 ccattgctgg acgagatggc gttgagcacg gccggctgat gctcggccaa cccagcgaca
       61 acggccgcca tctcggccag ttgcacctcg atcgccgcga cgcgtcgctc ggtcgcagcg
      121 tgatcagcac cgagcgtgtg tacatcggcg cggagatacg gcgcaacgga acgctcggcc
      181 acatggcgtg cttgtggaac tccaggagcc ttggtcagtc gccttagacc ctgcctcaac
      241 caacccattc gtgcaccttt ctcatcgcga caactcgctg aaccacgcca actggcagtc
      301 tagaggaggc ctcatccgct cgtggatagc caggcgccaa ccagagggag aacatattgg
      361 gttcaccacg gtcatacttg aagactcgag ttcgacggct gctcgaccga atcgcgcttc
      421 cgtatgtcga cgcnnnnnnn nnngtccagc acacaacgac gcctcagctg gaccaaccgg
      481 gcgacagtgc ctcgtcgatc gaagcaccac ggcacgggac gagcgactac tttcatcacg
      541 ccaaccacga gctgcgcacc cttgaactcg agcgagtacc gaagggggca acgcgcgcac
      601 tgagcgtcgg cgcgcaagga agatggtact tcgaatggtt cgaacgctcc gtcggaccgc
      661 tcgacgagca catcggcgtc gaagcctacg aggacaagcc caccgaccta cccgactatg
      721 tgacctgggt cgctgatacc gccgaccaca tggtgggtgt tgccgacgga tcggtcgatc
      781 tcgtcttcgc cggccaaacg tccgaacatc tgtgggcacg cgagttgtca ggcttcctga
      841 cagaggctca tcgggtgttg cggtccggcg gcgttctggc gctcgacagc ccgaatcgtc
      901 tcgtcactca acacctcttg tggagccacg gtggacacac cattgagttg tcggcggcag
      961 agatgaaaga gttgctgaac ctggccgggt tcgaagttct ctccgttacg ggtatctgga
     1021 actgcgtgat cgacggccgt atcgtcgcct tggaatacgg cattgacaac cccgccatct
     1081 tcgcccgccg cgccgcggcg ggcaccggac gccccgacga ctcctttgtg tggtggatca
     1141 acgctcgccg gtctgatcgc accatcgatg acgtgacgct gcgcgctcgt gtcgaccagt
     1201 tttttgaagc acattggaat acccgggttt cccgaggtct tgcacctgat tgtctgaacc
     1261 tcaatgggcc gcttgacatt gtgccgggaa cactgagtga aaccctgccg ttcccactcc
     1321 atcaaggcac gtggacggtc tcgttgcggc tggccgtcgg cacctggaat gacgtgagtg
     1381 gattcggagt ccgcatcgtg gccccccgtg accacttgat ccacgatctg cccctctcgg
     1441 cggcaaccgt caccgacagc gaggcaacct ggacgatcga gcagccgtat ctcctgttcg
     1501 ccctgacgct gcagatccga gcagacgacg tgagcggccc ggtatcgatt cgtctgccca
     1561 tcgaggtcgc attccaatcg ggttgatcaa tgactgctcc ggaagcgggt gctgaggggc
     1621 gagtagcgga ggatgcagaa gatcgcgcgg aggccgtcgc gccagccgat cttcttgcct
     1681 tcggcatagg tgcgggcgct gtacgagatg ccgacctcgt agatgcgcca gttgcctcgg
     1741 gcgaccttgg cggccaactc gggctcgatg ccgaagcgtt gctcctgcag ttcgaacgac
     1801 tgcagcacct cgcggcggaa cgccttgtag cagctctcca tgtcggtgag gttcaggttg
     1861 gtgaacacgt tcgacaacgt ggtaaggaac cgattgccga ccgagtgcca gaagtacagc
     1921 acgcgatggg cgtgggtggt caggaatcgt gagccgaaca cgacatcggc acggccgtcg
     1981 agcagaggct gcatcatgtg cacgtaatcg gccgggtcgt actccagatc agcgtcctgg
     2041 atgatgacga acggctgtgt cgccgcggca aacccacggc gcaacgccgc acccttgccc
     2101 tggttgggcg actggcggat cagcttgacc cgcgggtcgg agatcgcctc gacgatggcg
     2161 gccgtgtcat cggtcgagga gtcgtcgacg acgatcaact cggcggtgta ggggctcgcc
     2221 aagacagcac cgatgatctc ggcgatcgtg gcctgctcgt tgtacgcggg catcaccacc
     2281 gagagttgag ctggcgactg atccggcacc tgatccggca gctgatccgg caggggcgca
     2341 gaaccgacga cggcagattc gggagcggac ggcatcgccg cgatgctagc gatggcccga
     2401 ccgcgatcgc cgaccgtcgc gccgtcacca caacctggcg tcacaacatg gcatcacgac
     2461 atctcgccgc ggcgatgcgc tacttggtca tcgccacctt gtactcgcgg aactggccga
     2521 cacaatcgcc gttgtcgatc gtttcgcggt aggtgccttc catcgcggtc gcggccagca
     2581 acccgttgga ggcggtggcc gtggtggcat gaaagaccaa caggaacgtc gcttcggtct
     2641 cgacgccgtt gcacagcggt gcctgctcac tgctgagctg gccacgagca gcgtacgacc
     2701 catcggcgaa ggcaagcacc ccgttggcgc gattgccgga gacctgggtc gaatccggga
     2761 acgacagcga cagcgacgac agttcgaggc agttgatgat ggtgcactgc gcccgctcga
     2821 cggtcaccgt gtagtcaccc agcggtgccg cggcgcggaa cgagtcctcg tccatcggga
     2881 acaggttgcg gatcgcgtcg aggtcgcttt gcaccgcgtt gagctggccc tgcacctcgg
     2941 ccaccgcggc agcctgggac tgttgggcag cctccgcatc ggctgcgcgc cctgcgcgtt
     3001 cgttacccag gtcggtcgtc agctggccga cctggccggt gagctcgtcg atcttggcct
     3061 gcgcatcgtt caattgttgg cggagatcga catcggggtc ctggatcgaa tcgagctgct
     3121 cggtcaagtc ggcgatctcg cgttgggccg cctgnnnnnn nnnnnnnnng cgagcgtcga
     3181 ggtaggcgac caccagtccg gacacagcag ccgccgcaag acagccggcg atgatcgcaa
     3241 cgacgcggcg cgccgtgttg ggccggggtg ctggcctggt ctcggcggcg atgagcgacg
     3301 ctccacagct gaagcagaag tggtcgtcgg ggccgacccg ttcaccgcaa cgggggcacg
     3361 ggcgctgagc aggtgacggc gacgttggca cggtcatcgc cacatcgtc
//
LOCUS       DAORNF010000335         3392 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_21056_length_3392_cov_1.784837, whole genome shotgun sequence.
ACCESSION   DAORNF010000335 DAORNF010000000
VERSION     DAORNF010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3392
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21056_length_3392_cov_1.784837"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="PLV68_13065"
     CDS             <1..331
                     /locus_tag="PLV68_13065"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HRE02226.1"
                     /translation="ADQASGWRYAGIHVDWLTDAGLWSRAVRIDSGFVRNVALFVPAG
                     ALITWTSRRPIVTVAAVATLSLLIEITQRTWLLGAPDVSDMIANTAGAAIGTVIALAA
                     ILSSKAR"
     gene            351..584
                     /locus_tag="PLV68_13070"
     CDS             351..584
                     /locus_tag="PLV68_13070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02227.1"
                     /translation="MATTMQVSELSRDRLRELGRPGATLEETLVDALDALEEQQFWKA
                     ADAAAARRAGLPNDQRAAIEAAESDVQRWLDAL"
     gene            581..871
                     /locus_tag="PLV68_13075"
     CDS             581..871
                     /locus_tag="PLV68_13075"
                     /inference="COORDINATES: protein motif:HMM:NF014505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HRE02228.1"
                     /translation="MIPAAGDVYLADARDETRRQVLVVSDSRLHRATGRAIVAPVIEH
                     VEGDDSPWRVGIDERFAVDYLTTLPLDRLLERVGRVDALTVRQARRAVTASL"
     gene            complement(899..2812)
                     /locus_tag="PLV68_13080"
     CDS             complement(899..2812)
                     /locus_tag="PLV68_13080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRE02229.1"
                     /translation="MSKPKSQTGQSRQEGDDDMALMRDNASAVRTTTVGPGRAAAGVM
                     PTEKSKNFGASARRLLRMMRPDRVRLVGVVVLGVASVVLSVIGPKVLGHATDIVFSGV
                     LSGAGINFASLHRTLWMVIALYLGSSVLAYTQSYVIAGVVQRLMHRLRADIEDKLNRL
                     PLSYVDRQPRGDLLSRVTNDVDNISQSLQQTMSQVLSAVLTVAGVTIMMLTISPLLAI
                     IAIVTIPLSMWMVKFIAARSRKRFIAQWKHTGMLNAQIEETFSGHNIVKAFGRQREVE
                     QRFAQTNQALYEASFHAQFASGTIQPAMGFLGNLNFVMIAVFGGLRVASGSMSLGDVQ
                     AFIQYSRQFTMPLSQFASMFNVLQSGIASAERCYELLDAPEQSADDTSDALGPNAAGR
                     VAFEHVTFSYNPDQPLIQDLSLVAEPGQTVAIVGPTGAGKTTLVNLLMRFYELDGGRI
                     TLDGHDIAKMSRDDLRSKMGMVLQDTWLFGGTIRDNIRYGNLEASDDDVMAAARATYV
                     DRFVHSLPDGYDTVLDDEGSSVSAGEKQLLTIARAFLADPTILILDEATSSVDTRTEV
                     LIQQAMGALRASRTSFVIAHRLSTIRDADVILVMEQGRIVEQGSHDELLARDGAYARL
                     YNAQFAGAATDVD"
     gene            complement(2809..>2988)
                     /locus_tag="PLV68_13085"
     CDS             complement(2809..>2988)
                     /locus_tag="PLV68_13085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02230.1"
                     /translation="DQILVLEDGRPVGLGPHERLLVDCPTYAEIVASQLGQQAAELGL
                     ATAGAAGVAEEGGGS"
     assembly_gap    2989..3034
                     /estimated_length=46
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3035..3379)
                     /locus_tag="PLV68_13090"
     CDS             complement(<3035..3379)
                     /locus_tag="PLV68_13090"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRE02231.1"
                     /translation="MWRRIGLVPQRPFLFSGTVASNLRYGKPDATDDEIWEALTIAQA
                     ADFVSAMPGGLDAPITQGGTNVSGGQRQRLAIARALVRKPEIYLFDDSFSALDLATDA
                     RLRAALVPSTRDA"
BASE COUNT          563 a         1101 c         1113 g          569 t
ORIGIN      
        1 ggctgatcaa gcttcgggtt ggaggtacgc aggcatccac gtcgactggc tcaccgatgc
       61 aggcctgtgg agccgcgccg ttcggatcga cagcggcttt gtccgcaatg tcgccctgtt
      121 cgtcccggct ggtgccttga tcacatggac gtcccgcagg cctattgtga ccgtggccgc
      181 ggtcgccacg ctctcgctgt tgatcgagat cacccagcgc acgtggctgc tcggcgcccc
      241 cgacgtcagc gacatgatcg ccaacactgc tggagcagcg atcggcaccg tcatcgccct
      301 cgccgccatc ctgtccagca aggcacgatg accggcgcta tagtcgggct atggcgacca
      361 cgatgcaggt cagtgaactg agccgcgacc gactccgcga actcggtcga ccgggagcga
      421 cgttggagga gacccttgtc gatgctctcg acgcgctgga agaacagcag ttttggaaag
      481 ctgccgatgc cgccgcagcg cgccgtgccg gtctgccaaa cgaccagcga gccgccatcg
      541 aggcagcaga gtccgacgtg caacgctggc tcgacgcact gtgattccgg cggccggcga
      601 cgtctacctc gcggacgcgc gagacgagac acgacggcaa gtcctcgtcg tgtccgactc
      661 gcggctacac cgagcgaccg gacgagcgat cgtcgctccc gtcatcgaac acgtcgaagg
      721 cgatgactcg ccgtggagag tcggaatcga cgagcggttc gccgtcgact acttgacgac
      781 gttgccgctc gaccggctcc tcgaacgcgt cggtcgcgtc gacgcgctga cagttcgaca
      841 ggctcgacgg gcggtcaccg cctcgctgtg acgacccatc ggacgtcgga gaggtgggtc
      901 aatcgacgtc ggtagcggcg ccggcgaact gggcgttgta caaccgagcg taggcgccgt
      961 cgcgggcgag gagctcgtcg tggctgcctt gttcgacgat gcggccctgc tccatcacca
     1021 ggatgacgtc ggcatcgcgg atggttgaca ggcggtgggc gatcacgaag ctggttcgcg
     1081 aggcgcgcag tgcgcccatg gcctgttgga tcagcacctc ggtgcgggtg tcgaccgagc
     1141 tggtcgcctc gtcgaggatc aggatcgtcg gatcggccag gaaggcccgg gcgatcgtca
     1201 gcagctgctt ctcgccagcg ctgaccgacg atccttcgtc gtcgagcacc gtgtcgtatc
     1261 cgtcgggcaa cgagtgcacg aaccggtcga cgtaggtcgc ccgcgccgct gccatcacgt
     1321 cgtcgtcgct cgcttccaag ttgccgtagc ggatgttgtc acggatcgtt ccgccgaaca
     1381 gccacgtgtc ctgcaatacc atgcccatct tggaacgcag gtcgtcacgc gacattttgg
     1441 cgatgtcgtg cccgtcgagg gtgatccgcc caccgtcgag ttcgtagaag cgcatcagca
     1501 ggttgaccag cgtggtcttg ccggcgccgg tcgggccgac gatcgccacc gtctgccccg
     1561 gctcggccac cagcgacagg tcctgtatca acggctggtc ggggttgtac gagaacgtga
     1621 cgtgttcgaa cgcgacccga cccgcggcgt ttgggccgag cgcgtcgctg gtgtcgtcgg
     1681 ccgactgttc gggcgcgtcg agcagttcgt aacagcgctc ggccgaggcg atgcccgact
     1741 gcaacacgtt gaacatcgag gcgaactggc tgagcggcat cgtgaactgg cgtgagtact
     1801 ggatgaacgc ctgcacgtcg cccaacgaca tcgaccccga cgcgacccgc aacccgccga
     1861 acacggcgat catcacgaag ttgaggttgc ccaagaaacc catcgccggc tggatcgtgc
     1921 ccgacgcgaa ctgggcgtgg aagctcgctt cgtacaacgc ctggttggtc tgggcgaagc
     1981 gctgctcgac ctcgcgttga cggccgaaag ccttgacgat gttgtgtccg ctgaaggtct
     2041 cttcgatctg cgcgttgagc atgccggtgt gcttccactg ggcgatgaag cgcttgcgcg
     2101 acctggcggc gatgaacttg accatccaca tcgacagcgg gatcgtgacg atggcgatga
     2161 tcgccagcag cggcgagatc gtgagcatca tgatcgtcac accggcaacc gtgagcacgg
     2221 ccgacaggac ctggctcatc gtctgctgca ggctctggct gatgttgtcg acgtcgttgg
     2281 tgacgcggct gagtaggtcg ccgcggggtt gacgatcgac gtagctgagc ggcaggcgat
     2341 tgagcttgtc ttcgatgtcg gctcgcagcc ggtgcatgag ccgctgcacc acacccgcga
     2401 tcacatacga ctgggtgtat gccagcaccg acgagccgag gtacagcgcg atcaccatcc
     2461 acagcgttcg gtgcagcgac gcgaagttga tacctgcgcc ggagagcaca ccgctgaaca
     2521 cgatgtcggt ggcgtggccc agcaccttgg ggccgatcac cgacaagacc acgctggcca
     2581 caccgagcac caccacgccg accaatctga cccgatcggg acgcatcatg cgcagcaggc
     2641 ggcgcgccga ggcgccgaaa ttcttcgact tctccgtcgg catcacaccc gctgcggccc
     2701 gtcctgggcc gacggtggtg gtacgcactg cgctcgcgtt gtcgcgcatc agcgccatgt
     2761 cgtcgtcgcc ttcttggcga gactgacctg tctgcgactt cggcttgctc acgacccgcc
     2821 tccctcctcg gcaacacccg ccgcgccagc ggtggccagc ccgagttcgg ccgcttgttg
     2881 gccgagctgc gaggccacga tctcggcgta ggtggggcag tcgaccagca gccgttcgtg
     2941 cgggccgagc ccgaccgggc ggccgtcctc gagcaccagg atctggtcnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntgcatc gcgggtagag ggcacgagcg
     3061 cggctcgcag ccgggcatcg gtcgccaggt cgagcgcgga gaacgagtcg tcgaacaggt
     3121 agatctcggg cttgcgcacc aacgccctgg cgatggcaag acgttggcgc tggccgcccg
     3181 acacgttggt gccgccctgg gtgatcggcg cgtcgaggcc acccggcatc gccgacacga
     3241 agtcggccgc ctgcgcgatc gtcagcgcct cccagatctc gtcgtcggta gcgtccggtt
     3301 tgccgtagcg caggttgctg gccacggtgc cggagaacag gaacggccgc tgcggtacca
     3361 acccaatccg gcgccacaac acctcggggg cg
//
LOCUS       DAORNF010000336         3391 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_21066_length_3391_cov_2.380695, whole genome shotgun sequence.
ACCESSION   DAORNF010000336 DAORNF010000000
VERSION     DAORNF010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3391
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21066_length_3391_cov_2.380695"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            662..1804
                     /locus_tag="PLV68_13095"
     CDS             662..1804
                     /locus_tag="PLV68_13095"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HRE02232.1"
                     /translation="MPIEWEGRQASAFVPTLLASRNLQLDERTVAAAARARDAVEQAG
                     EAMAAGVAGLASMLLRAESLSSSAIAGVTTNPLDLAVAEMDPLHPPSAASKWVAANVA
                     VMADAVGVGQAGPLTTATLRRWHRTLMAASPTPVRGVGKLRTQQGWIGGTSPLDADLV
                     PPPPDMVPALLDDLIAYVNRDDLDPVAQAANAHAQFAIIHPFADGNGRIGRALVAWML
                     TRNLALVAPPPVSMRLVPRVHSYHSGFVLFRIGDHTAWVNRFIRAVASAARTQESMAA
                     SVASLNERWRKQLFALRPADERPIRRDAAAWRALELLPVHLVLTGQRIHDEVGVSMRS
                     SHLALHELLSAKVVVERVTAPGGRSGGPPRLYTNTDLLDLVTPPRH"
     gene            1867..>3391
                     /gene="ettA"
                     /locus_tag="PLV68_13100"
     CDS             1867..>3391
                     /gene="ettA"
                     /locus_tag="PLV68_13100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HRE02233.1"
                     /translation="MAHEYIYTCYKLARHYPPDRTVLENISLSFYPGAKIGVLGSNGA
                     GKSSLLRIMAGLDDGYTGEARLTPGFTAGYLAQEPQLDPSKDVLGNVMDGVAEIKSVL
                     DRYDEVMAMWADPDADYDKVGALQAELEDKIAATDAWSLERNVEIAMDALRCPPNDAD
                     VSTLSGGERRRVALCRLLLSHPDLLLLDEPTNHLDAESVEWLERFLQDYSGTVVAITH
                     DRYFLDNVAEWILELDRGRGFPFEGNYSSWLEQKQERLEREEKGNDKRRRALERELEW
                     VRMSPKARQSKGKARLAAYDRLAAEAEAAERGPDKLEIMIPAGRRLGDTVIEVSHLAK
                     GFGDRLLIEDLSFSLPRAGIVGIIGPNGAGKTTLFKMLTGQEPTDAGTITIGDTVELA
                     YVDQGRDNLDPDKTVYEEITDGIEFMRVGNREINGRAYVASFNFKGSDQQKKVGDLSG
                     GERNRVHLAKLLKTGGNVLLLDEPTNDLDVDTLRALENALEAFPGCAVVISHDRWFLD
                     "
BASE COUNT          598 a         1176 c         1119 g          498 t
ORIGIN      
        1 gccaccctgt tcgtcgcatg aacctcgacc ttcggtcgga gacgacgaac cagccaacgt
       61 tgagcgaaca cccggacagc accctgcccg ggtatcgccg cgtccaaccg gtgctcgcat
      121 ggctccacga ccggcacggc cgggttggtc accctgttcg tcgcatgaac caccaccccc
      181 ggtccgagac gaccaacgaa cccaagttga gcgaacacct ggacagcacc ctgtccgggc
      241 aacgccgcgt ccaaccggtg ctcgcatggg tccacgactg gcacgatcgc cagggtccca
      301 tccctccgga ggacccggcc gagcggcacc accgagccaa ccaccacgtt cgtcgcatca
      361 gcccccgacc tcggccggag acgacgaacg aaccaaagtt gagcgaacac ctcggcactc
      421 ccctgtccgg gcaacgccgt gtgcggccgg tgcttgtgcg acgtcaccac gggcagggct
      481 gcttggggga aaccacccag gcaagcccga cgagccagta ccgtgacaag cctggtcgcc
      541 ccatgaggct tggcctctgg tcggcaaccg caggcgctgc ccaagccagt ggtgctgggg
      601 tgtgtggcgg ctgggacagg cgtttggaga ggggtgagcg gtggacgtag caggcagcga
      661 ggtgccaatc gagtgggaag ggcgacaggc gtcggcgttc gtgccgacgt tgctggcttc
      721 gcggaacctt caactggacg agcggacagt ggccgccgcg gcacgggcgc gggatgcggt
      781 cgagcaggct ggcgaggcga tggctgcggg ggttgccgga ctggcgtcga tgctgttgcg
      841 ggccgagagt ctgtcgtcgt cggcgatcgc cggcgtgacg accaatccac tcgacctcgc
      901 ggtcgctgag atggaccctc tccatccacc gtcagcggcg tccaagtggg ttgcagccaa
      961 cgtggcggtg atggccgacg cggtcggcgt cgggcaggca ggtccgctga cgaccgccac
     1021 cctgcgtcgt tggcaccgca cgctgatggc cgcgagcccc acaccagtac ggggcgtcgg
     1081 caagctgcgc acacagcagg ggtggatcgg cgggacatcg ccactcgatg ccgacctggt
     1141 gccaccgccg cctgacatgg tgccagcgtt gctggacgat ctgatcgcgt acgtcaaccg
     1201 cgacgacctc gacccggtgg cgcaggcagc gaacgcacat gcgcagttcg ccatcatcca
     1261 ccccttcgcc gatggcaacg gccgcatcgg ccgcgcgctt gtcgcgtgga tgctcacccg
     1321 caacctcgcg ctggtggcgc caccgccggt cagcatgcgc ttggttcccc gggtccacag
     1381 ctaccactcg ggcttcgtgc tgttccgcat cggcgatcac accgcctggg tcaaccgatt
     1441 cattcgggcg gtggcaagcg ccgccagaac gcaggagagc atggcagctt cggtcgccag
     1501 cctgaacgag cggtggcgga agcagttgtt cgcgctgcgc cctgcagacg agcggcccat
     1561 tcgccgcgac gcggccgcgt ggcgggcact ggaactgctc cctgttcatc tcgtcctgac
     1621 cggtcagcgc atccacgacg aggttggcgt gtcgatgcga tcgtcacacc tggcgctgca
     1681 cgaactgctc agcgccaagg tcgtggtcga gcgggtcacc gcgccgggcg gccgatccgg
     1741 tgggccgcct cggctctaca ccaacaccga cctgctcgac ctcgtcaccc caccccggca
     1801 ctgaagcggt ctcagtggat gggacggggc agtcggtcgg aggccggcgg tagcctcgac
     1861 aatcgcatgg ctcacgagta catctacacc tgctacaagc tcgcccgtca ctatccgccc
     1921 gatcgcacgg tgctggagaa catctcgctc tccttctacc ccggtgccaa gatcggtgtg
     1981 ctcggctcca acggtgccgg caagtcgagc ctgttgcgga tcatggccgg gctcgacgac
     2041 ggctacaccg gcgaggcccg tctcacgcca ggcttcacag ccggctacct ggcccaagag
     2101 ccccagctcg acccgagtaa ggacgtgctc ggcaacgtga tggacggtgt ggccgagatc
     2161 aagtcggtgc tcgatcgcta cgacgaggtc atggcgatgt gggccgaccc cgacgccgac
     2221 tacgacaagg tcggcgccct gcaagccgag ctcgaagaca agatcgccgc caccgacgcg
     2281 tggagcctgg agcgcaacgt cgaaatcgcc atggatgcgc tgcgttgtcc ccccaacgac
     2341 gccgacgtgt cgaccctgag cggtggcgag cgccgccgcg tggcgctgtg ccggttgctg
     2401 ctcagccatc ccgatctgtt gctgctcgac gagccgacca accacctcga tgccgaaagc
     2461 gtcgagtggc tggagcggtt cttgcaggac tactcgggca cggtcgtggc gatcacccac
     2521 gaccgctact tcctcgacaa cgtcgccgag tggatcctcg agctcgaccg cggccgcggg
     2581 ttcccgttcg agggcaacta ctccagctgg ctcgaacaga agcaagagcg gttggagcgc
     2641 gaggagaagg gcaacgacaa gcgccgtcgt gcgctcgagc gcgagctcga gtgggtgcgg
     2701 atgtcgccca aggcccgcca gtcgaagggc aaggcccgcc tcgccgccta cgaccgcctc
     2761 gccgccgagg ccgaagcagc cgagcgcggc cccgacaagt tggagatcat gatcccggcc
     2821 gggcgccgcc tgggcgacac cgtgatcgag gtgtcgcacc tggccaaggg cttcggcgac
     2881 cggctgttga tcgaagacct gtcgttctcg ctgccccgcg ccggcatcgt cggcatcatc
     2941 ggccccaacg gcgccggtaa gacgacgctg ttcaagatgc tcaccgggca agagccgacc
     3001 gacgcgggca cgatcaccat cggcgacacg gtcgaactgg cctacgtcga ccagggccga
     3061 gacaacctcg accccgacaa gaccgtgtac gaagagatca ccgacggcat cgagttcatg
     3121 cgcgtcggca accgcgagat caacggccgg gcctacgtcg ccagcttcaa cttcaagggc
     3181 agcgaccagc agaagaaggt cggagacctg tcgggcggcg aacgcaaccg cgtccacctc
     3241 gccaagctgt tgaagaccgg cggcaacgtg ctgctgctcg acgaaccgac caacgacttg
     3301 gacgtcgaca cgctgcgcgc cctggagaac gcgctggaag ccttccccgg ctgcgcggtg
     3361 gtgatcagcc acgaccgctg gttcctcgac c
//
LOCUS       DAORNF010000337         3390 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_21075_length_3390_cov_1.488156, whole genome shotgun sequence.
ACCESSION   DAORNF010000337 DAORNF010000000
VERSION     DAORNF010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3390
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21075_length_3390_cov_1.488156"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    259..294
                     /estimated_length=36
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <295..>776
                     /locus_tag="PLV68_13105"
     CDS             <295..>776
                     /locus_tag="PLV68_13105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02234.1"
                     /translation="LAGSVANAVVRFDRHRVSLVAVFSDLIRNGAALVGVAFNSIGRH
                     SQGGILRERVIPRILEADPDELVASDTGLIDPAKVLAVGRRDEKPGGHGDRAGALAAV
                     ELACWDLLAKYHDEPAAATIARAFGRVRAEGAAVYAAGGYYSPTGGIDALRAEVAGYL
                     "
     assembly_gap    777..891
                     /estimated_length=115
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <892..1416
                     /locus_tag="PLV68_13110"
     CDS             <892..1416
                     /locus_tag="PLV68_13110"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HRE02235.1"
                     /translation="GRFGRDDAIAYADALKPYDLRWYEEPADPQDYALIDELTERFPN
                     AIATGENLFSVQDADNLLRYGGLRPGIDVVQMDAGLSYGLTEYAAMIASLERCGFDRT
                     AAIPHGGHLLNLHIVTGLGLGGCEAYPEVFQPFGGYSSTCRLIDGRMIPGDEPGFGLE
                     AKPNLAVEIDRIMR"
     gene            1476..>1765
                     /locus_tag="PLV68_13115"
     CDS             1476..>1765
                     /locus_tag="PLV68_13115"
                     /inference="COORDINATES: protein motif:HMM:NF014601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HRE02236.1"
                     /translation="MGSVYAAGLHGAGHDVIAIDPWPAHVEAINASGLRVSGPRGEQV
                     AAICAFTQVSDVPAEQVDLVIVAVKSAQAGAAAVRIAPLLSPTTIVLTIQNGL"
     assembly_gap    1766..1927
                     /estimated_length=162
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1928..2384
                     /locus_tag="PLV68_13120"
     CDS             <1928..2384
                     /locus_tag="PLV68_13120"
                     /inference="COORDINATES: protein motif:HMM:NF020135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ketopantoate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HRE02237.1"
                     /translation="AGADVERVVAAWRRGGFDTHPAADVRAMQWEKLICNVAYSAPCA
                     LTGMTVGEVRDDKHLGAVSRAAATEAWQTARALNVPIAVDDPVAFARDFAAAMPAAKP
                     SLLLDIEARRASEIEFINGAVPREAAKCDLAAPVNATLTALVKAREASW"
     gene            2464..>3000
                     /locus_tag="PLV68_13125"
     CDS             2464..>3000
                     /locus_tag="PLV68_13125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRE02238.1"
                     /translation="MTTNEQFEVRGFNHVAMVCADMARTVDFYTNILGFRLMKTVDLP
                     DAGGQHFFFDVGNGNSLAFFWFPEAPDGVPGLSAPKTQPGFGDWTSATSSLNHIAFDV
                     PADKFEEYHKKLKATGVGVGPIVNHDNSPSQVSWEMNDDVYVRSFYFQDPDGVLLEFA
                     CWTREFTPADVANAGRTAA"
     assembly_gap    3001..3038
                     /estimated_length=38
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3172..>3390
                     /locus_tag="PLV68_13130"
     CDS             3172..>3390
                     /locus_tag="PLV68_13130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02239.1"
                     /translation="MTGHDQAGKAVVASDEWVDPVTSPILPGSEFHLLWGSDTEARFP
                     DDGSRPAVGAYFPPLGGFRFGQFCVPPDS"
BASE COUNT          496 a          990 c         1049 g          504 t
ORIGIN      
        1 gcgcatcgag cgcgagtgca aggtgccggt gcggatgtcg ccgatgccgc tggaagcggt
       61 cgtgctcggc gccggccact gcatcgagca ctacgaagcc ctcaagggca tgttcatggg
      121 cgccagacgc tgacgcaact gcggtgccgg cccggctccg cgtcaggtac ggacgaatcg
      181 attggtctat acgtatcggt agccggaacc gtgtgccgga gcacacgtca gcacacggtg
      241 cggtacggac agaggggcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngctggc
      301 gggttcggtc gcgaacgcgg tcgtccgctt cgaccgtcac cgcgtctcgc tcgtcgcggt
      361 gttcagcgat ctgatccgca acggcgcggc gctggtcggg gtcgccttca actcgatcgg
      421 ccgccactcg caaggcggca tcctgcgcga gcgggtgatc ccacgtatcc tggaggccga
      481 cccggacgaa ttggttgcgt ccgacacggg cctgatcgac ccggccaagg tgttggcggt
      541 gggacggcgc gacgagaagc ccggtggcca cggcgatcgc gccggtgcgc tggccgcggt
      601 cgaactcgcg tgctgggacc tgctcgccaa gtatcacgac gagcccgccg cggcgaccat
      661 cgcccgagcc ttcggccggg tgcgcgcgga gggggcggcc gtgtacgcgg ccggtggcta
      721 ctacagcccg accgggggta tcgacgccct gcgggccgag gtcgcgggct atctcgnnnn
      781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nggtcgcttc
      901 ggccgcgacg atgccatcgc ctatgccgac gcgctcaagc cgtacgacct gcgctggtac
      961 gaagaaccgg ccgaccccca ggattacgcg ctgatcgacg agttgaccga gcgcttcccc
     1021 aacgcgatcg cgaccggtga gaacctgttc tcggtgcaag acgcagacaa cctgttgcgc
     1081 tacggcgggc tgcgccctgg catcgacgtc gtacagatgg acgccgggct cagctacggg
     1141 ttgaccgagt acgcggcgat gatcgcgtcg ctcgaacgtt gtggcttcga ccgtaccgcg
     1201 gcgatcccgc acggcggcca tctgttgaac ctgcacatcg tcaccggtct cggactcggc
     1261 ggttgcgagg cgtatcccga agtgttccaa cccttcggcg gatacagctc gacgtgccgg
     1321 ttgatcgacg gacgaatgat ccctggcgac gaacccggct tcgggctgga agccaaaccc
     1381 aacctggccg ttgagatcga tcggatcatg cgatgacggg cagcggctcg actggtgcgg
     1441 acgcgatgcg aatcgccgtc gtcggctgcg gcgcgatggg gtcggtgtac gcagccggtc
     1501 tccacggcgc cggtcacgac gtgatcgcga tcgacccgtg gcccgcgcac gtcgaggcga
     1561 tcaacgcgtc cgggctgaga gtgtctggcc cccgtggcga gcaggtcgcc gcgatctgtg
     1621 ctttcaccca ggtttccgat gtgcccgccg aacaggtcga cctggtgatc gtcgccgtca
     1681 agtccgcgca ggcgggcgct gccgctgtcc gcatcgcgcc gctgctcagt ccgacgacga
     1741 tcgtgctcac gattcagaat ggcctnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnggc gggtgccgac gtcgagcggg tcgttgccgc atggcggcgc ggcggtttcg
     1981 atacccaccc cgccgccgac gtacgggcga tgcagtggga gaagctgatc tgcaacgtcg
     2041 cctacagcgc gccgtgcgcg ctgaccggca tgaccgtcgg cgaggtgcgc gacgacaagc
     2101 accttggcgc ggtcagtcgt gctgccgcga ccgaagcgtg gcagaccgcc cgcgcgttga
     2161 acgtgccgat cgcggtcgac gatccggtcg cgttcgcccg cgacttcgct gccgcgatgc
     2221 ccgcggccaa gccgtcgctg ttgctcgaca tcgaggcccg ccgggccagt gagatcgagt
     2281 tcatcaacgg cgccgtgcca cgcgaggctg cgaagtgcga tttggccgcg ccggtcaatg
     2341 ccacgctgac cgcgctggtc aaagcccgtg aggcgagttg gtgagcgatg cacgctcgac
     2401 acgccaactt caccacaacg atggaacgat cagtagctct gaatcgatca gaaaggcacg
     2461 tccatgacaa ccaatgagca gttcgaggtt cgaggcttca accacgtcgc catggtgtgc
     2521 gccgacatgg cccgcaccgt cgacttctac accaacatcc tcggcttccg cttgatgaag
     2581 acggtggacc tgccggatgc gggcggccag cacttcttct tcgatgtcgg caatggcaac
     2641 tcgctggcgt tcttctggtt ccccgaagcg ccggatggtg ttcccggcct gtcggctccg
     2701 aagacgcaac cgggctttgg cgactggacg agtgcgacgt cgtcgttgaa ccacatcgcg
     2761 ttcgacgtgc cggccgacaa gttcgaggag taccacaaga agctcaaggc aacgggtgtg
     2821 ggcgtcggcc ccatcgtcaa ccacgacaac tcgccgtcgc aggtgtcgtg ggagatgaac
     2881 gacgacgtct acgtgcggtc gttctacttc caggacccag acggagtgct gctcgagttc
     2941 gcctgttgga cgcgtgagtt cacgccggcc gatgtggcta acgccggtcg caccgcggcc
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnca ccaccatcga cgagtgggcc
     3061 cagtcccact cctgaccacg acgccgaggt acgcaccgca cacgtgaaag actgcgggct
     3121 cgacgagagc gaggggctgt aatggttgaa acgcgtgcgg tgcggcgggt tgtgacgggg
     3181 cacgaccagg caggcaaggc ggtggtcgcg agcgacgagt gggtcgatcc ggtgacatcg
     3241 ccgatcttgc cgggtagcga gttccatctg ctgtggggga gcgacaccga agctcgcttc
     3301 cctgacgacg gcagccgccc ggccgtcggg gcgtacttcc cgccgttggg agggtttcgc
     3361 ttcggccagt tctgcgtgcc gccggacagc
//
LOCUS       DAORNF010000338         3389 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_21081_length_3389_cov_1.718656, whole genome shotgun sequence.
ACCESSION   DAORNF010000338 DAORNF010000000
VERSION     DAORNF010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3389
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21081_length_3389_cov_1.718656"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>949
                     /locus_tag="PLV68_13135"
     CDS             <1..>949
                     /locus_tag="PLV68_13135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02240.1"
                     /translation="PKPGVGAAVRLPVAGRGAIPVDAVAVALNVTMTETSQPGFVQVF
                     PTGGAVPGSSSNVNASAAGQTIPNAVVTPLGADGSITLFTSGGTHLLADVSGYFVAAD
                     APVAAGRFSAITPRRVFDTRPEFSIGYSGDKPAPLSRVTVDVVGAAGLMVGDTGAVAV
                     NITPTESTLPGYIQAAAAGGLVVGASSNLNIERSGQTIANMAIVPVGDDGSIELLTTG
                     GAHLLVDVLGWFSTDEVAVSTSGLYVPVSPERVLDTRPASAVNTDAEQRPGLGDTTKP
                     GRGGQVSVFFDGLIPIEPAAVVVNITTTEATLPGYVQAAG"
     assembly_gap    950..970
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1140..2624)
                     /locus_tag="PLV68_13140"
     CDS             complement(1140..2624)
                     /locus_tag="PLV68_13140"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HRE02241.1"
                     /translation="MRASTRLQWRRQRTKLVGLAALAVTGLVAFADVQVTPVVGRGGT
                     APTGVVAGQSILGSIDLFGTVAESGGIGGAAVVHQMAIDFAPDDYRAMIAAYVADGTK
                     RYIRATVVIDGTRIDNVGVRLKGNSTLEDAVFFPQPEEDGDIVFDDDAAEAAGSNALD
                     FNTEVADDPSLVPLLIRFDWFVAGQRYQGYDEIAMRFAAYGMAGFNEAVGLEMIGAVG
                     EPVQQWAYSTVTINGGPAQLRLLVEVPTAAFVARNFDHPGVLYRATGESLFDFVGDDP
                     ALYADQFEQIAPPGPVDLTPVIDLIRWTGQASDDEFAAGLAERVELDSLAEYLAAHTT
                     INNWDDMGGGGRNYYLWYDLTTRRFTVVSWDVNLAFYRSVFRQRDADARADQPLPAPV
                     QDDNGDWFVDLQPVLVGDPGSNNLLKTRFLDTPAFAARYDAAVAADCATLIDGGAAAS
                     SFAAIVQTVLASGAVTPADIHDEVDVMRTALVLKSDQCQRTPTN"
     gene            complement(2652..>3389)
                     /locus_tag="PLV68_13145"
     CDS             complement(2652..>3389)
                     /locus_tag="PLV68_13145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02242.1"
                     /translation="ASIVGGAASCDATTEMTCTINNLTNGTNYQFTVAAINQLGTGPS
                     FIAGPVKPGTTPTNPINLTATGDNNQVTLSWALTNEGGYPVTYTITVDGDQTFTTTNR
                     TYTVQNLVNFQSYSFTVSAANEIGPAATSATATGTPVPCKVTSYKKPTGTVAINTTTG
                     NNSAKIEFEIKTDKKCPGGLQVELVRKNDGVVVYTGTLTPKSSKSYGKYESSVSANTV
                     PVGFGSGGLWVRLVNTGDEHSLNVSGG"
BASE COUNT          513 a         1165 c         1121 g          569 t
ORIGIN      
        1 cccaagccgg gggtgggtgc cgccgtgcgg ttgccggtgg ccgggcgagg cgccatcccc
       61 gtcgatgccg tggccgtcgc gctcaacgtc accatgaccg agacctcgca gcccggcttc
      121 gtgcaggtgt tccccaccgg tggagcggtg ccggggtcgt cgtccaacgt caacgcgtcg
      181 gccgccggcc agacgatccc gaacgccgtc gtcaccccgc tcggcgccga cggcagcatc
      241 accctgttca ccagcggagg cacccacctg ctggccgacg tcagcggata cttcgtcgct
      301 gcggatgcac cggtcgcggc cggccggttc agcgcgatca caccccgccg cgtgttcgac
      361 acccggcccg agttctccat cggctacagc ggcgacaagc ccgcgccgtt gtcacgggtg
      421 acggtcgacg tcgtcggcgc ggccgggctg atggttggtg acaccggtgc ggtcgcggtc
      481 aacatcaccc ccaccgagtc aaccctgccc ggttacatcc aggccgccgc ggcgggcggg
      541 ctcgtggtcg gcgcgagctc caacctcaac atcgagcgca gcgggcagac catcgccaac
      601 atggcgatcg tcccggtcgg cgacgacggc tcgatcgaac tgctcaccac cggcggtgcc
      661 cacctgctgg tcgacgtgct cggatggttc agcaccgacg aggtggcggt gtcgacgtcg
      721 ggcctgtacg tacccgtgtc acccgagcgc gtgctcgaca cccgacccgc ctccgcggtc
      781 aacaccgatg ccgaacagcg ccccggcctc ggcgacacga ccaagcccgg ccgcggcggc
      841 caggtttcgg tgttcttcga cggcctgatc cccatcgaac ccgcggccgt cgtcgtcaac
      901 atcaccacga ccgaagccac cctgcccggc tacgtccaag ccgcaggatn nnnnnnnnnn
      961 nnnnnnnnnn gagctcgtcg gtcaacatcg aacgggccgg ccagaccatc gccaacgccg
     1021 cgatcacccc cctcggcgcc gctcatggtc tgtcgatcta cacccagtcc ggcacccatc
     1081 tgatcgcgga catctccggc ttcttccgca cctgaatctt cacggccgcc gtatcggcgt
     1141 cagttggtcg gggtgcgctg gcattggtcg gacttcagga cgagcgcggt acgcatgacg
     1201 tcgacctcgt cgtggatgtc ggcgggggtc acggcaccgc tggccagcac ggtctggacg
     1261 atggcggcga aggacgacgc tgctgcaccg ccgtcgatca acgtggcaca gtcggccgcc
     1321 accgccgcgt cgtagcgcgc agcaaacgct ggggtgtcga ggaaccgcgt cttgagcagg
     1381 ttgttcgaac ctgggtcacc gaccagcacg ggttggagat cgacgaacca atcaccgttg
     1441 tcgtcctgca ccggcgcggg caacggctgg tcggcgcggg cgtcggcatc gcgctggcgg
     1501 aacacgctgc ggtagaacgc gaggttgacg tcccacgaca cgaccgtgaa ccgtctcgtc
     1561 gtcaggtcgt accacaggta gtagttgcgc ccaccgccgc ccatgtcgtc ccagttgttg
     1621 atcgtggtgt gcgcggcgag gtactcggcc agcgagtcga gctcgacccg ctcggccaga
     1681 ccggcggcga actcgtcgtc gctggcttgg ccggtccagc ggatcaggtc gatcaccggg
     1741 gtgagatcga ccgggccggg cggggcgatc tgttcgaatt ggtcggcata cagagccggg
     1801 tcgtcgccga cgaagtcgaa caggctctcc cccgtcgccc ggtacaacac gccggggtgg
     1861 tcgaagttgc gggccacgaa cgccgccgtc ggcacctcga ccagcagccg taactgggcc
     1921 gggccgccgt tgatcgtcac ggtcgagtag gcccactgct gcaccggctc gccgacggcg
     1981 ccgatcatct ccaatccgac cgcctcgttg aatccggcca tgccgtatgc agcgaaccgc
     2041 atcgcgatct cgtcgtagcc ctggtagcgc tggccggcga cgaaccagtc gaagcggatc
     2101 agcagcggca ccaacgacgg atcgtcggcg acctcggtgt tgaagtccaa cgcattgcta
     2161 ccggccgctt ccgccgcgtc gtcgtcgaag acgatgtcgc cgtcctcttc gggctgcggg
     2221 aagaacacgg catcctccag cgtcgagttg cccttcagac gaacccccac gttgtcgatg
     2281 cgggtgccgt cgatcaccac cgtggcccgg atgtaccgct tggtgccgtc ggccacgtag
     2341 gccgcgatca tcgcccggta gtcgtctggg gcgaagtcga tcgccatctg atggacgacg
     2401 gccgcgcccc caataccccc gctctcggca acggtgccga acagatcgat gctgccgagg
     2461 atcgactgcc ccgcgacaac gccggtcggg gccgtgcctc cacgacccac gaccggcgtc
     2521 acctgaacgt cggcaaaggc caccagtccg gtcaccgcca gcgcggccaa gcccacgagc
     2581 ttggtgcgct ggcggcgcca ttgcagccga gtcgacgccc tcatgcagga tgtgcgcaac
     2641 gccgaagcgc gtcagccgcc cgagacgttg agcgagtgct cgtcgcccgt gttgaccagc
     2701 cgcacccaga gtccacccga gccgaagccg accggcaccg tgttggcgga gaccgacgat
     2761 tcgtacttgc cgtacgactt gctgctcttg ggcgtcagcg tgccggtgta cacgaccacg
     2821 ccgtcgttct tgcggacgag ttcgacctgc aagccgccgg ggcacttctt gtccgtcttg
     2881 atctcgaact cgatcttggc gctgttgtta cccgtggtgg tgttgatcgc caccgtgccg
     2941 gtcggcttct tgtagctggt gaccttgcac ggcaccggag taccggtcgc cgtcgcggac
     3001 gtggcggcgg ggccgatctc gttcgcggcc gacacggtga agctgtagct ctggaagttg
     3061 accaggttct gcacggtgta ggtgcggttc gtggtggtga aggtctggtc gccgtcgacg
     3121 gtgatggtgt aggtcaccgg gtagccgcct tcgttggtca gcgcccagct cagcgtcacc
     3181 tggttgttgt cgcccgtcgc ggtgaggttg atcgggttgg tgggcgtcgt gcccggcttg
     3241 accggaccag cgatgaacga cgggccggtg ccgagctggt tgatcgccgc caccgtgaac
     3301 tggtagttgg tgccgttggt gaggttgttg atggtgcagg tcatctcggt cgtcgcgtcg
     3361 caggatgctg caccgccgac gatgctcgc
//
LOCUS       DAORNF010000339         3368 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_21250_length_3368_cov_1.891941, whole genome shotgun sequence.
ACCESSION   DAORNF010000339 DAORNF010000000
VERSION     DAORNF010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3368
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21250_length_3368_cov_1.891941"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="PLV68_13150"
     CDS             complement(<1..417)
                     /locus_tag="PLV68_13150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02243.1"
                     /translation="MVTPADTMRDRPARAVVAVRPGALVVGGAVQPFVAGDATSMLRN
                     YLTLVRSMRNQKRAAEIDLRRDDIQVLAAATGQAPEAVLDRLATLMGATRTQRTALLA
                     VFATGALVLTLATSAAASPAADAPPTSLTPVPQPAAA"
     gene            complement(627..>1416)
                     /locus_tag="PLV68_13155"
     CDS             complement(627..>1416)
                     /locus_tag="PLV68_13155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010081119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HRE02244.1"
                     /translation="IEWSAVDLTDLDALGANWPADTRLIWLETPTNPLLTCIDIEAVA
                     AIAHARGALVVVDNTFATPYLQQPLTLGADIVVHSATKYLGGHSDVVGGFVAVNDDGL
                     AERIGYTQNAAGAVPAPFDCYLLLRGLKTLAVRMDRHCANARAIVDLLVNHPKVERVL
                     YPQLPDHPGHRAAAKQMSDFGGMVSFTLRGGEQAALEVVSRTQLFTLAESLGAVESLI
                     EHPGRMTHASAAGSPLQVPDSLVRLSVGIESTADLVADLEQALG"
     assembly_gap    1417..1429
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1430..1766)
                     /locus_tag="PLV68_13160"
     CDS             complement(<1430..1766)
                     /locus_tag="PLV68_13160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HRE02245.1"
                     /translation="MGEQEVQQGWGFETRAIHAGQDPEPTTGAVVTPISLSTTFAQDG
                     VGNHKGFEYSRSGNPTRAALETCVASLERARHGFAFASGLAAEDNVLRLLQRGDRVML
                     GNDAYGGTFR"
     gene            complement(1759..3231)
                     /locus_tag="PLV68_13165"
     CDS             complement(1759..3231)
                     /locus_tag="PLV68_13165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HRE02246.1"
                     /translation="MAWGDDAADRRLHGTNIAESVLDTIGNTPLVRLKRISELDDLPC
                     VLAMKMETTNPGGSSKDRPALEMVLAAERDGLLRPGGTIVEPTSGNTGVGLAIVAAQR
                     GYRCVFVMTDKVAPEKVSLLRAYGAEVVVCPVAVAPEDPTSYYSVAERLTHELNAFRP
                     NQYANPNNPLAHQKTTGPELWQQTGGRITHFIAGAGTCGSITGTARYLREMNPNVKII
                     AADPEGSVFSGGSGRPYLVEGVGEDFFPAAWERDLYDEIIPISDQESFLTARRVSRDE
                     GILIGGSGGMAVAAAIQVAKRANPDDIVVVFNPDSGRGYLSRVFDDAWMANFGFVTEC
                     DLCVGAMLASRGSMPELLYVNPSHTVRDAIDVMRTHEISQLPVCKNTPPFAAAEVSGA
                     VDELDLMDAIFRDPGVLDREVDAVMGPKLPTIGVGQKIELAVHMLDTAPALLVLSGGR
                     PLSVITRSDVLAYFQNQSALPVGTSSNDSNDSNDGGTHRG"
BASE COUNT          500 a         1094 c         1196 g          565 t
ORIGIN      
        1 cgccgcggcc ggctggggta ccggggtgag cgaggtcggc ggcgcatcgg ccgccggtga
       61 agcagccgcc gatgtcgcca aagtgagcac cagggcacct gtggcgaaca cggcgagcag
      121 tgccgtgcgc tgggtgcggg tcgctcccat cagcgtggcc aggcgatcga ggacagcctc
      181 cggcgcttgg ccggttgccg ccgccaacac ctggatgtcg tcgcggcgca ggtcgatctc
      241 cgcggcccgc ttctggttgc gcatcgagcg caccaacgtg aggtagttgc gcagcatcga
      301 ggtcgcatcc cctgcgacga agggctgcac ggcacctccg acgacgagcg cacccggtcg
      361 gacggcaacg acggcgcgcg ccgggcggtc acgcatggtg tctgctggtg tcaccatccc
      421 gttgcctcct cccttcgtcg ccggcccatc acatcgctcg tcacatcatc tcgtcacatc
      481 attcggtgca cgagttcgat catcggctca cgtcatgacc gacatgagcc cgaacaccgc
      541 gaccgtaccg agttgcactg ctcgtgtcga gagcttggca cacgatgcat agccggtggc
      601 agcagcgtgg ccgggtgagc cgcgtgtcag cccagcgctt gttcgaggtc ggcgacgagg
      661 tcggcggtcg attcgatgcc gaccgacagg cgcaccagcg agtcgggaac ctgcagcggt
      721 gagccggcgg ccgacgcgtg ggtcatccgc ccggggtgct cgatgaggct ctcgaccgcg
      781 ccgagcgact cggccaacgt gaacaactgg gtgcgcgaca ccacctcgag cgcagcctgc
      841 tcgccacccc ggagggtgaa gctgaccatg ccgccgaagt cgctcatctg cttggccgcg
      901 gcgcggtggc ccgggtgatc gggcagctgc ggatacagca cccgctcgac cttggggtgg
      961 ttgaccagca ggtcgacgat ggcccgggcg ttggcgcaat ggcgatccat gcgcaccgcc
     1021 agggtcttga ggccgcgcag cagcaggtag cagtcgaacg gggcgggcac cgcgccggcg
     1081 gcgttctggg tgtagccgat ccgctcggcc aacccgtcgt cgttcaccgc gacgaaaccg
     1141 ccgaccacgt cgctgtggcc accgaggtac ttggtggccg agtgcacgac gatatcggcc
     1201 cccagtgtca gcggctgttg caggtagggc gtggcgaagg tgttgtcgac caccaccaag
     1261 gcaccgcgag cgtgggcgat cgccgcgacc gcctcgatgt cgatgcaggt gagcagtggg
     1321 ttggtcggcg tctccagcca gatcaggcgg gtgtcggcag gccagttggc gccgagcgcg
     1381 tcgaggtcgg tgagatcgac cgcgctccac tcgatgnnnn nnnnnnnnng gcggaacgta
     1441 ccgccgtatg catcgttgcc gagcatcacc cggtcgccgc gctggagcag gcgcagcacg
     1501 ttgtcttcgg ctgcgaggcc actggcgaag gcgaacccgt ggcgggctcg ttccaacgag
     1561 gcgacgcatg tctccagcgc ggcgcgggtg gggttgccgc tgcgtgagta ctcgaagccc
     1621 ttgtggttgc cgacgccgtc ctgggcgaag gtcgtgctga gcgagatcgg tgtcaccact
     1681 gccccggtgg tcggctcggg gtcttggcca gcgtgaattg cccgagtctc gaaaccccaa
     1741 ccctgttgta cttcctgctc acccacggtg ggtgcctccg tcgttgctgt cgttgctgtc
     1801 gttgctgctg gtgccgaccg gcagcgcgga ctggttctgg aagtaggcca gcacgtcgct
     1861 gcgggtgatg accgacagcg gtcgtccacc cgacagcacc agcagggcag gagccgtgtc
     1921 cagcatgtgt accgccagtt cgatcttctg gccgacaccg atggtgggca gcttggggcc
     1981 catcacggcg tcgacctcgc ggtcgagcac gccggggtcg cggaagatgg cgtccatcag
     2041 gtcgagttcg tcgaccgcgc ccgacacctc ggccgcagcg aacggcggcg tgttcttgca
     2101 cacgggcagt tggctgatct cgtgggtgcg catgacgtcg atcgcgtcgc gcacggtgtg
     2161 gctggggttg acgtacagca actcgggcat gctgccacgg ctggcgagca tcgcccccac
     2221 gcacaggtca cactcggtga cgaacccgaa gttggccatc cacgcgtcgt cgaacacgcg
     2281 ggacagatag ccgcggccag agtccgggtt gaacaccacg acgatgtcgt cagggttggc
     2341 ccgcttggcg acctggatcg cggcggcgac ggccatgcct ccgctgcccc cgatgaggat
     2401 gccttcgtcg cggctgacgc ggcgggcggt gaggaagctc tcctggtcgc tgatcgggat
     2461 gatctcgtcg tacaggtcgc gctcccacgc ggcggggaag aagtcttcgc cgacaccttc
     2521 gaccaggtac ggccgaccac tcccgccgct gaacaccgag ccttcggggt cggcggcgat
     2581 gatcttgacg ttggggttca tctcgcgcag gtaccgcgct gtgccggtga tgctgccgca
     2641 ggtacccgcc ccggcgatga agtgcgtgat ccgcccgccg gtctgctgcc acagttcggg
     2701 gccggtggtc ttctggtggg ccagggggtt gttggggttg gcgtattggt tgggccggaa
     2761 cgcgttgagc tcgtgggtga gccgctcggc cacgctgtag tagctggtgg ggtcttcggg
     2821 ggccaccgcc accgggcaga ccaccacctc ggcaccgtag gcgcgcagca gcgacacctt
     2881 ttcgggggcg accttgtcgg tcatgacgaa cacgcagcgg tagccacgtt gggcggcgac
     2941 gatcgccagc ccgacgccgg tgttgccgct ggtcggctcg acgatggtgc cgccggggcg
     3001 gagcaagccg tcgcgctcgg ccgcgagcac catctccaac gccgggcgat ccttcgagga
     3061 gccaccgggg ttggtggtct ccatcttcat cgccagcacg cagggaagat cgtcgagttc
     3121 actgatccgc ttcagccgca ccagcggtgt gttgccgatc gtgtcgagga ccgattccgc
     3181 gatgttcgtc ccgtgcaagc ggcggtcagc cgcgtcgtca ccccatgcca tgtcggccag
     3241 ggtagtgcgc ccccggagat cgcggccgag tacggcggcg ctgtggtcga agaccaggag
     3301 gccacatcag gcagtacggc gccctcatgg ctgtgacacc cttccggtac cttcggtggg
     3361 gtggtgat
//
LOCUS       DAORNF010000340         3366 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_21264_length_3366_cov_1.734823, whole genome shotgun sequence.
ACCESSION   DAORNF010000340 DAORNF010000000
VERSION     DAORNF010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3366
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21264_length_3366_cov_1.734823"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="PLV68_13170"
     CDS             complement(<1..210)
                     /locus_tag="PLV68_13170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02247.1"
                     /translation="MSAVKRWAANVRHPLRWLTGVCGGESAYPLVILFGLNAVDELDR
                     AAFGILLPEIREAFDLDLTGVLTLVT"
     gene            complement(207..>976)
                     /locus_tag="PLV68_13175"
     CDS             complement(207..>976)
                     /locus_tag="PLV68_13175"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRE02248.1"
                     /translation="LSVTTLAFGMSVTSWLLNDRFFGWIPRQRLKVAPLFGRLDIDTP
                     TRFYAFSVVVLAVVVLLVRGVRRSRVGRVIVAMRENERGVQAFSVSTVRAKLTAFMIS
                     GAIAGVAGALFVHMNHAFSLGQFNIGRSFTALTSAIIGGLGSITGVVIGATYIGVTSR
                     LPDEWRILATSLSVLLILLVMPSGLGSQIVKVRDIIARAAQRRAGIEANGDPSVAAAD
                     AAEAAAAVRSAATNRATDGANLLDGTPAATMLEGAAE"
     assembly_gap    977..1011
                     /estimated_length=35
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1012..>1388)
                     /locus_tag="PLV68_13180"
     CDS             complement(<1012..>1388)
                     /locus_tag="PLV68_13180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02249.1"
                     /translation="RRDTDVTSSWQGAEEVRPLDPLTARLAWVRLARFGSWAVVAAAV
                     VLAPVVLRVDRLIQLNETVIFCIIGVSMLVLTGWAGQISLGQMGFVAVGGALSALLTS
                     RWDVDLTLALLVAAAAGALAALL"
     assembly_gap    1389..1547
                     /estimated_length=159
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1548..2376)
                     /locus_tag="PLV68_13185"
     CDS             complement(<1548..2376)
                     /locus_tag="PLV68_13185"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRE02250.1"
                     /translation="MGVSSRIGAQTGDRRVARIAAVVAVAVALYIAFDRYPLPWGQAI
                     RAVVLGMLTAMLAVGLALVYRANRVLNFAQADLGSVPTALAVALVVFSGWSYSLGFVV
                     GLVAAVVLGGVVELAFVRRFHRASRLVLTVATLGITQLLVLVGILLPRWWGRNAASER
                     LPQFSSWYVEIGTVRLLSNDFVAMVTAPVVMALVALFLNHTRVGVAVRASAERSDRAN
                     MLGIPVNRLNTVVWAVAAALSYVALFLRAGILGFPIGSAFDVTNLLFAMSALVIGRLQ
                     "
     gene            2703..>3366
                     /locus_tag="PLV68_13190"
     CDS             2703..>3366
                     /locus_tag="PLV68_13190"
                     /inference="COORDINATES: protein motif:HMM:NF014803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HRE02251.1"
                     /translation="MPAQTPHVTSPKIEQFLASTDAATPFVVVDVDRVAERYDALTTA
                     LPGARVYYAVKANPGAAILRRLVECGSAFDVASPAEIDFCLAAGASAGDISYGNTIKK
                     RRDIAFAARAGVRRFTVDCLEELHKVADVLADSAGGIAGTSICVRLRHECAGADWPLS
                     RKFGCEAPDVENLLVAATELGMECGVSFHVGSQQRNPLVWNTALASAAAIFREMAERG
                     IQL"
BASE COUNT          565 a         1141 c         1043 g          423 t
ORIGIN      
        1 cgtgacgagc gtgagcacgc cggtgaggtc gaggtcgaag gcttcgcgga tctcgggcag
       61 caggatgccg aacgcggccc ggtcgagctc gtcgacggcg ttgagcccga acaagatcac
      121 caacggatag gcggactcgc caccgcacac gcccgtcagc cagcgcagcg gatggcgcac
      181 gttggccgcc cagcgcttca cggcgctcac tcggccgccc cttccaacat cgtggctgcg
      241 ggcgtgccgt ccaacaggtt cgccccgtcc gttgctctat tcgtggcggc actcctgaca
      301 gcggctgccg cctcggccgc gtccgcggca gcgaccgatg ggtcgccgtt ggcctcgatg
      361 ccggcccggc gctgtgcggc gcgggcgatg atgtcgcgca ccttgacgat ttggctgccg
      421 aggcctgacg gcatcaccag caggatcagc agcaccgaca gtgacgtggc caaaatgcgc
      481 cactcgtcgg gtaggcgtga cgtgacgccg atgtaggtcg caccgatcac gacgccggtg
      541 atcgacccga gcccaccgat gatcgcggag gtcagcgcgg tgaagctgcg cccgatgttg
      601 aactggccga gactgaatgc gtggttcatg tgcacgaaca gcgccccggc caccccggcg
      661 atcgcgccgc tgatcatgaa cgcggtcagc ttggcccgca cggtcgacac cgagaacgcc
      721 tgcacgccgc gctcgttctc gcgcatcgcc acgatcaccc gtccgacgcg cgaccggcgc
      781 accccgcgca ccagcagcac caccaccgcc agcacgacca cggagaaggc atagaagcgc
      841 gtcggggtgt cgatgtcgag ccgtccgaac agcggcgcga ccttcaaccg ttgacgcggg
      901 atccatccga agaagcggtc gttgagtaac cacgacgtga cgctcatgcc gaaagccaac
      961 gtggtcaccg acaggtnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nccagcagcg
     1021 ctgccagtgc cccggccgcg gccgcgacca gcaacgccag ggtgagatcg acgtcccagc
     1081 gcgacgtgag cagcgcgctg agcgcaccac cgaccgcgac gaagcccatc tggcccagcg
     1141 agatctggcc cgcccagccg gtgaggacca acatcgacac gccgatgatg cagaagatca
     1201 ccgtctcgtt cagttggatc agtcggtcga cgcgcaacac gaccggggcc agcacgacgg
     1261 ccgcggcgac cacggcccac gacccgaacc gcgccaggcg gacccacgcc agccgggcag
     1321 tgagcgggtc gagcgggcgc acctcttcgg caccttgcca cgacgaggtc acatcggtgt
     1381 cgcggcggnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntct gcagcctccc
     1561 gatcaccagc gccgacatcg cgaacagcag gttggtcacg tcgaacgcgc tgccgatcgg
     1621 gaacccgagg atgccggccc gcaagaacag ggccacgtac gacagcgccg ccgcgaccgc
     1681 ccacacgacg gtgttcaacc ggttgaccgg gatgccgagc atgttggccc gatcgctgcg
     1741 ctcggccgag gcccgcaccg cgacgccgac cctggtgtga ttgaggaaca gcgccaccaa
     1801 cgccatcacc accggcgcgg tcaccatggc gacgaagtcg ttcgacagca ggcgcacggt
     1861 gccgatctcg acgtaccaac tggagaactg cggcagccgc tcggacgccg cgttgcgccc
     1921 ccaccaccgc ggcagcagga tgccgaccag caccagcagc tgggtgatgc caagggtggc
     1981 caccgtcagc accagccgcg acgcgcgatg gaagcggcgg acgaaggcca gttcgacgac
     2041 accgccgagt accaccgccg ccaccaggcc gacgacgaag ccgagcgaat acgaccagcc
     2101 cgagaacacc accagtgcga ccgcgagcgc ggtcggcacc gatccgagat cggcctgagc
     2161 gaagttgagg acgcggttgg cgcggtagac cagcgccagg ccgacggcca acatcgcggt
     2221 cagcatcccg agcaccaccg cccggatcgc ctggccccat ggcagcgggt agcgatcgaa
     2281 ggcgatgtac aaggccaccg cgacagcgac cacagcggcg atgcgcgcga cgcggcgatc
     2341 acccgtctgc gccccaatcc ggctgctgac ccccattgcc tggggaacgt agccgatccc
     2401 cgatcaccgg tcccttacgg agcagcctcg ccgtaccggg tggaacgatc gaaaactttg
     2461 acaactcacc cgcgtcggtc gtggtcgcgg cgaaccgggt cgcagatgtc acctggtgct
     2521 ccgacggtcg attcgacacc gcgtctggcc cgcggcgatc ggttcagcgg tcgctccccg
     2581 gtccagcgag cgagtccctg atggcaggta tccgtcagcc tcgctgactg acgcgccagg
     2641 cgcgcctgac tggccgatca ccgagtagcg acaccggttc gacgtgttta acgtgcccca
     2701 ccgtgcctgc gcagaccccc cacgtcacca gtcccaagat cgagcagttc ctggcttcga
     2761 ccgatgccgc gacgcccttc gtggtggtcg acgtcgaccg tgtcgcagaa cgctacgacg
     2821 ctttgaccac ggccctgccg ggagcccgcg tctactacgc ggtcaaggcc aacccgggcg
     2881 ctgcgatcct gcgtcgcctg gtcgagtgcg ggtccgcctt cgacgtcgcc agcccggcag
     2941 agatcgactt ctgcctcgcc gccggcgcgt cggcgggcga catctcgtac ggcaacacca
     3001 tcaagaagcg ccgcgacatc gcattcgccg cacgggccgg tgtgcgtcgc ttcacggtcg
     3061 actgcctgga agagctgcac aaggtcgccg acgtgctggc cgacagcgcc ggtgggatcg
     3121 cgggcacgtc gatctgcgtc cggctccgcc acgagtgtgc gggcgcggac tggccgctgt
     3181 cgcgcaagtt cggttgcgag gctcccgacg tggagaacct gttggtcgct gcgaccgagc
     3241 tgggcatgga gtgcggcgtg tcgttccacg tcggctcgca gcagcgcaac ccgctcgtct
     3301 ggaacacggc gcttgcctcg gcggcggcga tcttccgcga gatggcggag cgaggcatcc
     3361 agctct
//
LOCUS       DAORNF010000341         3364 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_21278_length_3364_cov_1.712602, whole genome shotgun sequence.
ACCESSION   DAORNF010000341 DAORNF010000000
VERSION     DAORNF010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3364
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21278_length_3364_cov_1.712602"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="PLV68_13195"
     CDS             complement(<1..287)
                     /locus_tag="PLV68_13195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HRE02252.1"
                     /translation="MTTTADRLDAIYEDVVQLNQGEPEFHQAVREVLESLGPVIEKHP
                     EFAEHKIIQRICEPERQIIFRVPWQDDQGEVHINRGLRVEFNSALGPYKGGL"
     gene            complement(284..592)
                     /locus_tag="PLV68_13200"
     CDS             complement(284..592)
                     /locus_tag="PLV68_13200"
                     /inference="COORDINATES: protein motif:HMM:NF013427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HRE02253.1"
                     /translation="MPEDPQQQHLDDELRLALEQVDELEQEYNQLVTDHGAIQEDRDA
                     VRVVLESARQRVETARRAIERAAAGQTATCSRCGGAIEPERLDALPGVETCAHCAGAR
                     "
     gene            complement(684..>890)
                     /locus_tag="PLV68_13205"
     CDS             complement(684..>890)
                     /locus_tag="PLV68_13205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRE02254.1"
                     /translation="AADEAATPPMSHTGVQMVVVEAGLVQVSVGDDTPVLRVGDVVLA
                     ERAPVVGWRNLRPEPARLLWICRD"
     assembly_gap    891..992
                     /estimated_length=102
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<993..1821)
                     /locus_tag="PLV68_13210"
     CDS             complement(<993..1821)
                     /locus_tag="PLV68_13210"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRE02255.1"
                     /translation="MRSDRESFRGSFGIESLDAMLDGIRPGDNIVWITDRTPRYAAIE
                     RCFVEASLAAGLPVIDVVTGQGDLARERPAGVEIVDATSGSRASRPVALTDEIERRLA
                     ISPGAAFVIDGLSALVRRWGEERALAFFARMCPTMLQTGALTFWRAAPTTDADLVESV
                     RQVTQCLLELHDAQLRIVKAEGRPHAAQGTVYRVELHDGAISAVADPAAGRLARGLQS
                     VRRDLGLTQSYLADIAGVTPSAISQAESGRRGLALNTLIVLSEALGVSLDRLVGAPAP
                     "
     gene            complement(2037..2399)
                     /locus_tag="PLV68_13215"
     CDS             complement(2037..2399)
                     /locus_tag="PLV68_13215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HRE02256.1"
                     /translation="MSILASSLTLAHADYSWRQYSLCRDTDPDLFFPVGTTGHALTQI
                     ARAKEVCGECPVSIECLEFALETNQDSGIWGGLSEEERRVIRRRRAARSRLASGGAPP
                     KTSAGPSAGRSPRTPRRR"
     gene            2682..>3364
                     /locus_tag="PLV68_13220"
     CDS             2682..>3364
                     /locus_tag="PLV68_13220"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HRE02257.1"
                     /translation="MTVHVLAHRGASAAEPENTIAAFRRAAEMGADAVELDVRRTVDE
                     VLVVHHDAHLPDGRAIVATFAAELPASVATVTDALDACAGMWVNVEIKNDPRDPDFDP
                     TDAVADATIALLADRGEPHRWLISSFRLETIDRCRALAPEIRTAWLTAALPDDVVATL
                     VEHGHLALHPWYGTVTSELIEACHDVGIAVNTWTCDDPDAMRQLIGWGIDGICTNVPD
                     VALAVLAESR"
BASE COUNT          508 a         1072 c         1145 g          537 t
ORIGIN      
        1 agtccgccct tgtacggccc gagcgcgctg ttgaactcga ctcgcagacc acggttgatg
       61 tggacctcgc cctgatcgtc ctgccacggc acgcggaaga tgatctggcg ctccggctca
      121 cagatgcgct ggatgatctt gtgctcggcg aactcggggt gcttttcgat caccggcccg
      181 agcgattcga ggacctcgcg cacggcctga tggaactctg gctcaccttg gttcagttgg
      241 acgacgtctt cgtagatggc gtcgaggcgg tcggcagtcg tggtcatcgg gctcctgcac
      301 agtgggcaca ggtctcgacc cccggcaggg cgtcgagacg ttcgggttcg atggcgcctc
      361 cgcagcgcga acaagtcgcg gtttgaccgg cagcggcgcg ttcgatggcg cgccgagcgg
      421 tttcgacgcg ttggcgcgcc gattcgagca cgacgcggac ggcgtcgcgg tcttcctgga
      481 tcgcaccgtg gtcggtgacc aactggttgt attcctgttc gagttcgtcg acctgctcga
      541 gagccaggcg caactcgtca tcgagatgct gttgctgcgg gtcttcaggc accgcgggca
      601 cggtatgggc cgcgaacttt cgcatcgcgt cctgatagcg ctgctatcac ccgatgacgg
      661 gtctcctgac gacgagggcg tgctcagtcg cggcagatcc agagcaggcg cgccggctcg
      721 gggcgcaggt tacgccaccc gacgaccggc gcccgttcag ccagcaccac gtcgccgacg
      781 cgcaacacgg gtgtgtcgtc accgacgctg acctggacca ggcccgcttc gaccaccacc
      841 atttgcaccc cggtgtgcga catcgggggc gtggccgctt cgtcggcggc nnnnnnnnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngtggcgca ggggcaccga ccaggcggtc
     1021 gagcgatacg ccgagcgctt cggacagcac gatcagcgtg ttcagcgcca ggccgcgccg
     1081 accggactcg gcctgggaga tggcgctggg tgtgaccccg gcgatgtcgg cgaggtacga
     1141 ctgggtcagg ccgaggtcac ggcgcacgct ctgcagtccg cgggccagcc gtccggcggc
     1201 cgggtcggcg accgcgctga tggcaccgtc atgcagttcg acgcggtaca ccgttccctg
     1261 tgccgcgtgc ggacgtccct cggccttgac gatccgcaac tgcgcgtcgt gcaactcgag
     1321 caggcactgg gtgacctggc gcacggactc gacgaggtcg gcatcggtgg tgggcgcggc
     1381 ccgccagaac gtgagcgcgc cggtctggag catggtgggg cacatccggg cgaagaaggc
     1441 cagggcacgc tcttcgcccc agcgacgcac cagggcgctg aggccgtcga tgacgaacgc
     1501 ggcgccaggg ctgatcgcca gacggcgttc gatctcgtcg gtcagcgcga ccggacgtga
     1561 tgcccgtgac cccgaggtgg cgtcgacgat ctcgaccccg gcgggccgtt cgcgggccag
     1621 atcgccctgg cccgtcacca cgtcgatcac gggcaacccg gcggcgagac tggcctcgac
     1681 gaagcaacgc tcgatcgccg cgtagcgcgg ggtgcgatcg gtgatccaca cgatgttgtc
     1741 acccggacgg atgccgtcga gcatggcgtc gagcgactcg atgccaaacg agccgcggaa
     1801 cgattcccgg tcgctacgca tgggcggact atagcactgc ttatggctca cgttgtccgg
     1861 cgaaagcggt ggattggcac gctcactccg gtgcccaaac gtacgacgac ccggcgtcgc
     1921 cgccgggtcg tcgtaggtaa gaaggattgc ccggccacac ggccggtgtc gtcagccggc
     1981 gacgctcacc ggggtgatgc tggcgccgga catcgggttc ggcgtcgccc gtgcggtcat
     2041 cgccgccttg gcgtgcgcgg gctgcgtccg gcggatggcc cggcgctcgt cttcggaggt
     2101 gcccccccag acgccaggcg gctgcgggca gcccggcgcc ggcggatgac gcggcgctcc
     2161 tcttcggaga gaccgcccca gatgcccgag tcctggttgg tctccaaggc gaactcgagg
     2221 cactcgatgc tgaccggaca ctcgccgcag acttccttgg cccgggcgat ctgcgtcagc
     2281 gcgtggccgg tggtaccgac cgggaagaac agatcggggt cggtgtctcg gcagagcgag
     2341 tactgccgcc acgagtagtc ggcgtgggca agtgtgaggc ttgacgcgag aatggacacg
     2401 tgcaggctcc tgacgacggg aacgtgtggg gggatgtcgg cgtgctccgc tgaacgatcg
     2461 atctgccaga acgttggacg atggatcgat ctggggcggg gctggcgtgg cggaccattc
     2521 cgcagacagg ggtcaccgta cgtttcgtgg gccgacttga caagggcttc gtttcgggaa
     2581 tgtgtccaaa cggccaacat ttgataacgc ccatctcgga tagagatggg cgaccggggc
     2641 tgcgaatcac cgccgacgca cgattcgcga gaggatgagg catgaccgtg catgtgctgg
     2701 ctcaccgtgg tgcatccgca gccgaaccgg agaacaccat cgccgcgttc cgtcgggcgg
     2761 ccgaaatggg ggccgacgcc gtcgagctcg acgtgcgccg taccgtcgac gaggtcttgg
     2821 tcgtccatca cgacgcccat ctgcccgacg gtcgggccat cgtcgccacg ttcgctgccg
     2881 agctgcccgc gtcggtagcc accgtgaccg acgcgctcga cgcctgcgcg ggtatgtggg
     2941 tgaacgtcga gatcaagaac gacccccgcg atcccgactt cgatcccacc gatgcggtcg
     3001 ccgatgccac catcgcgctg ctcgcggacc gaggtgagcc gcatcgctgg ctgatctcgt
     3061 cgttccggtt ggaaaccatc gaccgctgcc gagctctggc gcccgagatc cgcacggcgt
     3121 ggctcaccgc tgcacttccc gacgatgtgg tggcgacgct ggtcgagcat ggccacctcg
     3181 ccctccaccc ttggtatggc acggtgacga gcgagttgat cgaggcctgt cacgacgtgg
     3241 gcatcgcggt caacacctgg acctgcgacg atcccgacgc gatgcgccaa ctgatcggct
     3301 ggggcatcga cggcatctgc accaacgtgc ccgacgtcgc gctggcggta ctggccgaat
     3361 cgcg
//
LOCUS       DAORNF010000342         3364 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_21279_length_3364_cov_1.526141, whole genome shotgun sequence.
ACCESSION   DAORNF010000342 DAORNF010000000
VERSION     DAORNF010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3364
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21279_length_3364_cov_1.526141"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>211)
                     /locus_tag="PLV68_13225"
     CDS             complement(<1..>211)
                     /locus_tag="PLV68_13225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016099277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02258.1"
                     /translation="VGTITEVYDYLRLLYARIGVQHCVNDGTRLARQTPQQIVDRIMQ
                     MDEGTRFQVLAPVVRGRKGEYKTLLE"
     assembly_gap    212..221
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<222..502)
                     /locus_tag="PLV68_13230"
     CDS             complement(<222..502)
                     /locus_tag="PLV68_13230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HRE02259.1"
                     /translation="MSDTIVVRGAREHNLRNISVDLPRDRLIVFTGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARQFLGQMDKPDVDVIEGLSPAISIDQKSASR"
     assembly_gap    563..602
                     /estimated_length=40
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<603..>1117)
                     /locus_tag="PLV68_13235"
     CDS             complement(<603..>1117)
                     /locus_tag="PLV68_13235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HRE02260.1"
                     /translation="SNIDTIQRIETLRALRLGEFDALVGINLLREGLDLPEVSLVCIL
                     DADKEGFLRSETSLIQTMGRAARNVDGQVIMYADNITQSMQRAIDETQRRRVLQQQYN
                     EANGINPQTIRKAVGDILSMLRPDSTAPVPGKDRRRQRERDKVQRELKTLPQHELARL
                     IQTLEEEMHEA"
     assembly_gap    1118..1282
                     /estimated_length=165
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1283..>2323)
                     /locus_tag="PLV68_13240"
     CDS             complement(<1283..>2323)
                     /locus_tag="PLV68_13240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441710.1"
                     /note="The UvrABC repair system catalyzes the recognition
                     and processing of DNA lesions. The beta-hairpin of the
                     Uvr-B subunit is inserted between the strands, where it
                     probes for the presence of a lesion; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HRE02261.1"
                     /translation="AQEMREFFPNNRVEYFVSYYDYYQPEAYIASSDTYIEKDSSIND
                     EIERLRHSATAGLLTRRDTIVVASVSCIYGMGSPEEYRGQMLDLNVGIDYDQRSILRR
                     LVDMQFDRNDMTLGRGKFRVRGDTIEIHPSYDDAVIRVEMFGDTIDRITTVDPLTGEQ
                     LGETQRVQIFPATQYAAGEERLRAAVVGIEAELQERLAWFESQGKLLEAQRLRMRTQY
                     DLEMMQEVGYCNGIENYSMHIDGRTYGQPPFTLLDYFPDDFLLVIDESHVAVPQLHGQ
                     YLGDLSRKDQLIEHGFRLPSARDNRPLRFEEVLERIPQAVFLSATPGDYELKVSSQVV
                     EQIVRPTGLTDP"
     assembly_gap    2324..2508
                     /estimated_length=185
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2835..2984
                     /estimated_length=150
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2985..3330)
                     /gene="coaE"
                     /locus_tag="PLV68_13245"
     CDS             complement(<2985..3330)
                     /gene="coaE"
                     /locus_tag="PLV68_13245"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HRE02262.1"
                     /translation="MIVVGLTGGIGSGKSSVSARLAALGAVIVDADATTRRLQEPGGS
                     LYVKIVERFGPEVVAADGTLDRAKIAAMVFPSPELLAELNAITHPGIGAELREQMRAH
                     VDNIDFDAAAKFE"
BASE COUNT          438 a          879 c          956 g          541 t
ORIGIN      
        1 cttcgagcag ggtcttgtac tcacccttgc ggccccgaac caccggcgcc agcacctgaa
       61 atcgggtgcc ctcgtccatc tgcatgatgc gatcgacgat ctgctgcggg gtctggcgag
      121 ccaggcgggt gccgtcgttg acacagtgct gcacgccgat gcgggcatac agcaggcgga
      181 ggtagtcgta gacctcggtg atcgtgccga cnnnnnnnnn nttacggctg gccgacttct
      241 gatcgatcga gatcgccggc gacagacctt cgatcacgtc gacgtcaggc ttgtccatct
      301 gccccaggaa ctggcgggcg taggcgctga gcgattcgac gtaacggcgt tgaccctcgg
      361 cgtagatcgt gtcgaaggcc aggctcgact tgccgctgcc cgacaagccg gtgaacacga
      421 tcagccgatc acgcggcagg tcgacgctga tgttgcggag gttgtgttcg cgggcgccgc
      481 gaacgacgat ggtgtcgctc attcgagtct cttcctaggg gcttcagccg gcttcgcgca
      541 gctcgcgacg cagttcggcg atnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      601 nnacgcttcg tgcatctctt cttcgagggt ttggatcagg cgggccaact cgtgctgcgg
      661 cagcgtcttg agttcgcgct gcaccttgtc gcgctcgcgc tggcggcggc gatccttgcc
      721 cggcaccggt gccgtcgagt cgggacgcag catcgagaga atgtcgccga ccgccttgcg
      781 gatggtctgc gggttgatgc cgttggcctc gttgtactgc tgctgcagca cccgccgccg
      841 ttgggtctcg tcgatcgccc gctgcatcga ctgggtgatg ttgtcggcgt acatgatgac
      901 ctgaccgtcg acgttgcgcg cggcgcggcc catggtctgg atcagcgacg tctcgctgcg
      961 caggaatcct tccttgtcgg catcgaggat gcacaccaac gacacctcgg gcaggtcgag
     1021 cccctcacgc agcaggttga tgccgaccag tgcgtcgaac tcgccgaggc gcagcgcacg
     1081 gagcgtctcg atgcgctgga tcgtgtcgat gttgctgnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nncggggtcg gtcagccctg tggggcggac gatctgctcg
     1321 accacctgcg acgacacctt cagctcgtag tcgcccggcg tggccgacag gaacacggcc
     1381 tgggggatgc gttcgagcac ctcttcgaag cgcaacgggc ggttgtcacg agcgctgggc
     1441 agtcggaaac cgtgctcgat cagttgatct ttgcgcgaca ggtcacccag gtactggccg
     1501 tgcaactgcg gcacggcgac gtggctctcg tcgatgacca gcaggaagtc gtcggggaaa
     1561 tagtcgagca gggtgaacgg cggctggccg taggtgcgcc cgtcgatgtg catcgagtag
     1621 ttctcgatgc cgttgcagta gccgacctct tgcatcatct ccaggtcgta ctgggtccgc
     1681 atccgcagac gctgtgcttc gagcagcttg ccctgcgact cgaaccaggc gagacgctcc
     1741 tgcagttcgg cctcgatgcc gacgaccgcg gcccgcagcc gctcttcgcc cgccgcgtat
     1801 tgcgtggccg ggaagatctg cacccgctgg gtctcgccga gctgctcgcc cgtgagcgga
     1861 tcgacggtgg tgatgcggtc gatcgtgtcg ccgaacatct ccacccgaat gaccgcatcg
     1921 tcatagctgg ggtggatctc gatcgtgtcg ccgcggacgc ggaacttgcc ccggccgagg
     1981 gtcatgtcgt tgcggtcgaa ctgcatgtcg accagacggc ggaggatgct gcgctggtcg
     2041 tagtcgatgc cgacgttgag gtcgagcatc tgaccccggt actcctcagg gctgcccatg
     2101 ccgtagatgc agctgaccga ggcgacgacg atcgtgtcgc gcctggtcag tagaccggcg
     2161 gtggcgctgt ggcggaggcg ttcgatctcg tcgttgatcg acgagtcctt ttcgatgtag
     2221 gtgtcgctgg aggcgatgta cgcctcgggc tggtagtagt cgtagtagct gacgaagtac
     2281 tcgacccggt tgttggggaa gaactcgcgc atctcctgcg cgannnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntc ggacaccacc
     2521 cgcaacgccg tctccatcac aagaggtgtt ggctcaaccg gtgttggctg catcgctctt
     2581 ggctcgatcc cccgaggagt gagggattgc gacacgtcgg gtgcagacac ggaaccaagt
     2641 gtatcaccga acgcctgtac cccggtaccc cgcggcactc gcgggcgtta ggcgagatgg
     2701 cctgctttgg gggtggtcgg gttgttgttg ccgagtcgtg ggtcggcggg atcgatcgcg
     2761 tccagcgact gcatccactg ccagagggcg gggatctgct cgtcgaggtc ggcgggggtg
     2821 ccggagttgt cgatnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngttcga acttggcggc
     3001 ggcgtcaaaa tcgatgttgt cgacgtgggc gcgcatctgc tcacgcagct ccgcgccgat
     3061 accggggtgg gtgatcgcgt tgagctcggc caacaactcg gggctcggga acaccatcgc
     3121 tgcaatcttg gcccggtcga gcgtgccgtc ggcagcgacc acctcggggc cgaagcgctc
     3181 gacgatcttg acgtacagcg agccgcccgg ctcttgcagc cgccgcgtcg tcgcgtcggc
     3241 gtcgacgatc accgccccca gcgccgccaa gcgggccgag accgacgact tgcccgaacc
     3301 aatgccaccg gtgagtccga ccacgatcat gtccccgacc gtatcgaagc gcaccgcccc
     3361 gggt
//
LOCUS       DAORNF010000343         3363 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_21285_length_3363_cov_1.878476, whole genome shotgun sequence.
ACCESSION   DAORNF010000343 DAORNF010000000
VERSION     DAORNF010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3363
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21285_length_3363_cov_1.878476"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="PLV68_13250"
     CDS             complement(<1..224)
                     /locus_tag="PLV68_13250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02263.1"
                     /translation="MNRVLSCIQPTGDVHLGNYLGALQPWVLNQHECDAFHGIVDLHA
                     LTITDKPGVLGQVTLDRRFGGAAGGATHAER"
     gene            335..>692
                     /locus_tag="PLV68_13255"
     CDS             335..>692
                     /locus_tag="PLV68_13255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HRE02264.1"
                     /translation="MAYDVSTPVFEGPFDLLLHLILREQVDIYEISLTRIVNAYLEEV
                     DKLQSVDLDLATEFLLIASTLVELKARRLLPGRADVDLDDEFALWEERDLLLARLLEC
                     KTFKDVATIFGRLADEA"
     assembly_gap    693..765
                     /estimated_length=73
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <766..1110
                     /locus_tag="PLV68_13260"
     CDS             <766..1110
                     /locus_tag="PLV68_13260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HRE02265.1"
                     /translation="PRHVERAYLRAITPKPVPTVDLFHVSAVTASVADAMTELLDELP
                     RAGRISFRRLTGELVERIEVIVRFLALLELFKQGMVELDQTERFGDIDIVWVADADAV
                     AAGAIPIDDYEG"
     gene            1113..1910
                     /gene="scpB"
                     /locus_tag="PLV68_13265"
     CDS             1113..1910
                     /gene="scpB"
                     /locus_tag="PLV68_13265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HRE02266.1"
                     /translation="MSTEFPTPIFSDPSADDAELGEASSDTSADASPDPSSSPSLGAD
                     VVRAIEAIVMVATEPVPTEQLAELLRQPAEVVERLCRGLAEAYDEAGHGFQFVAIAGG
                     WRYQSVPDLAAYVERFLLDGQRARMSGAALETLAIVAYKQPISRAQIASIRGVDPDGV
                     LRTLAARGYVTQVAIDPGPGQAALFGTTPQFLDKLGLHSLSDLPPLAEFVPGADVVEA
                     LEHGLRAPDADPKQLRNEPIGALLDEHGADEAAPDAGPGAASDHEGT"
     gene            1916..2767
                     /locus_tag="PLV68_13270"
     CDS             1916..2767
                     /locus_tag="PLV68_13270"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HRE02267.1"
                     /translation="MYRQTRIARPSPVAERGPRTEARDVEIPLWEQWEAERALATGER
                     LQKVLAARGWGSRRVCEDMIAAGRVRVNGEVAVLGRRVEVDTDVVEVDGVAVGVRPDT
                     VYYLLNKPAGVITTASDTHGRPTVVELVPAEPRVFPVGRLDADTEGLLLLTNDGDLAN
                     RIMHPSHGVDKEYLVFADREASPSALRRLRDGVELDDGITAPATASQPQPGVIRLVIH
                     EGRNRQVRRMCAAVGLDVQRLVRVRIGPLRDTNLAPGRFRTLTNAELRRLVEAVAEPP
                     QRYDRRS"
     gene            2764..>3363
                     /locus_tag="PLV68_13275"
     CDS             2764..>3363
                     /locus_tag="PLV68_13275"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HRE02268.1"
                     /translation="MNDATPSAARRANIIGLGLIGGSLGLALRERGWQVGGDDFDDER
                     RERALLMGVLDQIGLYDDAAITFVATPVLAVADQVKRALAETAGVVTDVGSVKGAITA
                     AIDDPRFVGGHPMAGSELEGLDGADAAMFQGAVWVLTPTARTADPAFAATAAVVAELG
                     GEVLALPPDRHDQLVAVVSHVPHLTAAALMGVASQRAEEH"
BASE COUNT          483 a         1102 c         1179 g          526 t
ORIGIN      
        1 cgttcagcgt gggtggctcc ccccgcagcg ccgccgaatc ggcgatcgag ggtgacctgg
       61 ccgaggacgc ccggcttgtc ggtgatcgtc agcgcgtgca gatcgacgat gccgtggaag
      121 gcatcgcact cgtgctggtt gagcacccac ggctgcagcg caccgaggta gttgcccaga
      181 tggacgtctc cggtcggttg gatacagctc agcacgcgat tcacgccgcc aggctaacga
      241 ggccatcgtt gtgcagggat gtgggttgtg cagggatgtg ggttgtgcac gggtgtgggt
      301 tctgctcggg tgtgggactc cgtatcgtgt gcgggtggcc tacgacgtgt cgacccccgt
      361 gttcgagggg ccgttcgacc tgttgttgca cctcatcctg cgtgagcagg tcgacatcta
      421 cgagatctcg ttgacgcgca tcgtcaacgc ctacctggaa gaggtcgaca agctgcagtc
      481 ggtcgacctc gacctggcca ccgagttctt gctgatcgcg tcgaccttgg tcgagctgaa
      541 ggcgcgccgg ctgttgcccg ggcgggccga cgtcgacctc gacgacgagt tcgcgttgtg
      601 ggaagagcgt gacctgctgt tggcccgtct gctcgagtgc aagacgttca aggacgtggc
      661 aaccatcttc ggtcggctgg ccgacgaggc cgnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnccccg ccacgtcgag
      781 cgcgcctacc tgcgcgcgat cacgcccaag ccggtgccca ccgtcgatct gttccacgtc
      841 tcggccgtga cggccagcgt cgccgatgcg atgaccgaat tgctcgacga actgccccgt
      901 gccgggcgca tctcgtttcg tcgcctgacc ggcgaactgg tcgagcggat cgaggtgatc
      961 gtacgcttcc tggccctgtt ggagctgttc aagcagggca tggtcgagct cgaccagacc
     1021 gagcgcttcg gtgacatcga catcgtgtgg gtcgccgacg ccgatgccgt ggcagccggg
     1081 gcgatcccga tcgacgacta cgaaggctga gcatgtccac cgagttcccc acaccgatct
     1141 tctccgaccc cagcgctgac gatgccgaac tcggcgaggc cagctccgac accagcgctg
     1201 acgccagccc cgatccgagt tccagtccca gccttggtgc cgatgtggtg cgcgcgatcg
     1261 aggcgatcgt catggtcgcc accgagccgg tgccgaccga gcagctggcg gagctgctcc
     1321 gtcaaccggc cgaggtggtc gagcggctgt gccgtgggtt ggccgaggcc tacgacgaag
     1381 cggggcacgg cttccagttc gtcgccatcg ccggtggctg gcggtaccag agcgtgcccg
     1441 atctggcggc ctacgtcgag cgcttccttc tcgacggcca gcgagcccgg atgagcggcg
     1501 cggcgttgga gacgctggcg atcgtggcgt acaagcagcc gatctcgcgg gcccagatcg
     1561 catcgatccg cggcgtcgac cccgacggcg tgctgcgcac gctggccgcg cgtggctacg
     1621 tgacccaggt ggcgatcgac ccgggtccgg gccaggccgc gctgttcggt acgactcccc
     1681 agttcctcga caagctcggc ctgcactcgc tgtccgacct gccgccgctg gccgagttcg
     1741 tacccggtgc cgacgtggtc gaagcgctgg aacacggcct gcgcgccccc gacgccgacc
     1801 cgaagcaact gcgcaacgaa ccgatcggtg ccctgctcga cgagcatggt gccgacgagg
     1861 ctgctccgga cgctggtccc ggtgccgcgt cggatcacga aggcacctga gcccgatgta
     1921 ccgccagacg cggatcgccc gcccgagccc cgtcgccgag cgcggcccgc gcaccgaggc
     1981 acgtgacgtc gagattccgc tgtgggagca gtgggaggcc gagcgggcgc tggccaccgg
     2041 cgagcgactg cagaaggtgt tggcggcgcg gggctggggc agtcgccggg tgtgcgagga
     2101 catgatcgct gccggtcgtg tgcgggtcaa cggcgaggtt gccgtgctgg gccgccgggt
     2161 cgaggtcgac accgacgtgg tcgaggtcga cggtgtggcc gtgggggtgc ggcccgacac
     2221 ggtgtactac ctgctcaaca agcctgccgg ggtgatcacc acagcgagcg acacccacgg
     2281 tcgtccgacg gtggtcgagc tggtgccggc cgagccacgc gtgtttcccg tcgggcgcct
     2341 cgacgctgac accgagggtc tgttgttgct gacaaacgac ggtgatctgg ccaaccggat
     2401 catgcatccg agccacgggg tcgacaagga gtacctcgtc tttgccgacc gcgaggcgtc
     2461 accgtcagcg ctgcgacgcc tgcgcgacgg cgtcgaactc gacgatggca tcacggcccc
     2521 ggccacggcc agccagccgc aacccggtgt gatccggttg gtgatccacg agggacgcaa
     2581 ccgccaggtg cgccggatgt gcgccgcggt cggcctcgac gtgcagcgcc tggtgcgggt
     2641 gcggatcggt ccgttgcgcg acaccaacct ggccccgggc cggttccgca cgctgaccaa
     2701 cgccgaactg cgccggttgg tagaagcagt cgccgagcca ccgcaacggt acgatcgacg
     2761 ctcgtgaatg acgctactcc gagcgcggcg cgccgggcca acatcatcgg cctggggttg
     2821 atcggcggct cgctgggcct ggcgctgcgc gagcgtggct ggcaggtggg cggcgacgac
     2881 ttcgacgacg agcgccgcga acgtgccctg ctgatgggcg tgctcgatca gatcggtctg
     2941 tacgacgacg cggcgatcac cttcgtcgcc acgcccgtgc tggcagtggc cgatcaggtc
     3001 aagcgggcgt tggcggagac ggccggtgtc gtcaccgacg tcggcagtgt caagggcgcg
     3061 atcaccgccg ccatcgacga cccgcgcttc gtcggcggtc acccgatggc ggggagtgag
     3121 ctcgagggcc tcgacggcgc cgacgcggcg atgttccagg gcgcggtgtg ggtgctgacg
     3181 ccgacggcgc gcaccgccga ccccgcgttc gccgccacgg ccgccgtcgt cgccgagctc
     3241 gggggagaag tgctggctct gccgcccgat cgtcacgacc agttggtggc cgtcgtcagc
     3301 cacgtgccgc acctgaccgc cgccgcgctg atgggcgtgg ccagccagcg ggccgaagag
     3361 cac
//
LOCUS       DAORNF010000344         3362 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_21301_length_3362_cov_1.745691, whole genome shotgun sequence.
ACCESSION   DAORNF010000344 DAORNF010000000
VERSION     DAORNF010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3362
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21301_length_3362_cov_1.745691"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>311)
                     /locus_tag="PLV68_13280"
     CDS             complement(<1..>311)
                     /locus_tag="PLV68_13280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02269.1"
                     /translation="LVAWPLGLITSTTFADGWSSMSHALSDPVVTHALQLTATVAVIT
                     VVVNVVFGVGVALLLVRYRFPGRRLLGALVDLPLSVSPVVVGLALTLAYGNRTGWFGG
                     A"
     assembly_gap    312..383
                     /estimated_length=72
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<384..1146)
                     /gene="cysT"
                     /locus_tag="PLV68_13285"
     CDS             complement(<384..1146)
                     /gene="cysT"
                     /locus_tag="PLV68_13285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HRE02270.1"
                     /translation="MTRGAASGLGLVMLTFSIVVLLPLAAVLVTTAEGGWPNYWNTIT
                     NPQTLAALRLTLTQALLVTLLNVVMGTLIAWVLVRDRFRGKRALDVVIDVPFALPTIV
                     AGLVLLALYGPQSPLGVNVANTQRAVFLALSFVTLPFVVRSVQPVLEQLDPDAEQAAA
                     TLGASRLTTFRRVVLPSLVPAIASGAGLSFARAISEYGSLVLLSGNLAMRTEVASVRI
                     LTYIESGNDAAAAAVATTLLAVALLVTVTLDVTQRR"
     gene            complement(1143..2303)
                     /locus_tag="PLV68_13290"
     CDS             complement(1143..2303)
                     /locus_tag="PLV68_13290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0043199 - sulfate binding [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HRE02271.1"
                     /translation="MWNPSHSGSLKTTNPIIFRSYLVTPSTSSPTPATSPVRRRWRTT
                     AAAVMAVMAGVGVMACGSSDAKGDGGGTSSTNLNLVGFAVPKAANNAMQAAFAKTPAG
                     AGVTWTESYGASGEQSRAVVAGLKADYVHFSLEGDVTRLVDSGQVAADWNAGPTQGIV
                     SDSVVVLVVRPDNPKGITGWADLIRDDIGIVTPNPGSSGAARWNILAAYGQVLANGGT
                     EQQAADYLTAFFEHTVALPGSGRDATTAFLAGTGDVLISYENEAILARQQGEKLEYFV
                     PDATLLIENPGAVLINADPKATAWLEFVLSPAGQTELVKKGFRPVIDGVAIGGADGIT
                     VDGANDPTNPFPTPSQLFTIADRFGGWKATNKAFFDADTGIVTLIQQKTGKQ"
     gene            2493..>3069
                     /gene="folP"
                     /locus_tag="PLV68_13295"
     CDS             2493..>3069
                     /gene="folP"
                     /locus_tag="PLV68_13295"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HRE02272.1"
                     /translation="MGVVNVTPDSFSDGGLHADVDDAVAHAKLLATQGALVVDVGGES
                     TRPGAGEVSVHEELARVVPVVERLAREASVLISVDTRKPEVAAQAVSAGAHLVNDVGG
                     LRDPEMVAACADLGVPVVVVHMRGEPAQMQVDPRYDDVVAEVTAHLRDRAGHALAAGV
                     ADVMVDPGLGFGKTVEHNLALLRALPLAIDHP"
     assembly_gap    3070..3118
                     /estimated_length=49
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          491 a         1062 c         1183 g          505 t
ORIGIN      
        1 gtgctccccc gaaccagccg gtgcggttgc cgtaggcgag ggtgagggcc aggccgacga
       61 ccacgggcga gaccgacagc ggcaggtcga ccagcgctcc cagcaggcgg cggccgggga
      121 agcgatagcg caccagcaac agcgccacgc cgacgccgaa gaccacattg accaccaccg
      181 tgatcaccgc gaccgtggcg gtgagctgca gcgcatgggt gaccaccggg tctgacagcg
      241 cgtgcgacat cgacgaccat ccgtcggcga aggtggtcga ggtgatcaat cccagcggcc
      301 aggcgaccag cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnncccggcg ctgggtcacg tcgagcgtga cggtcaccag
      421 caacgccacc gccaacagcg tggtggccac ggctgcggct gccgcgtcgt tgccgctctc
      481 gatgtaggtg aggatgcgca cggatgcgac ttcggtccgc atcgcaaggt tgcccgacag
      541 cagcaccagc gagccgtact cgctgatcgc ccgggcgaac gacagcccgg cacccgacgc
      601 gatcgcgggc accaggctgg gcagcaccac gcggcggaac gtggtgaggc ggctggcacc
      661 cagcgtggcg gcggcctgtt cggcatcggg atcgagttgt tccagcaccg gctgcaccga
      721 gcgcacgacg aacggcagcg tcacgaacga cagggcgaga aacacggctc gctgggtgtt
      781 ggcgacgttg acgccgagcg ggctctgggg gccgtacagc gccagcagca ccagtccggc
      841 gacgatggtc ggcagggcga agggcacgtc gatcaccacg tcgagggccc gcttgccccg
      901 gaaccggtcg cggaccagca cccatgcgat cagcgtgccc atcaccacgt tgagcagcgt
      961 gaccagcagt gcttgggtca gggtgaggcg cagcgcggcg agggtctgcg ggttggtgat
     1021 cgtgttccag tagttgggcc aaccgccctc ggcggtggtg accagcaccg ccgccagggg
     1081 gaggagcacg acgatgctga acgtcagcat caccaacccg agaccgctcg ccgcgccacg
     1141 ggtcattgct tgcctgtctt ctgttggatc agggtgacga tgccggtgtc cgcgtcgaag
     1201 aaggccttgt tggttgcctt ccagccgccg aagcggtcgg cgatggtgaa caactgggat
     1261 ggagtgggga aggggttggt cgggtcgttg gcaccatcca ccgtgatgcc gtccgcgcca
     1321 ccgatcgcca ctccgtcgat caccggccgg aagcccttct tgaccagttc ggtctgtccg
     1381 gccgggctga gcacgaattc gagccacgcc gtggccttgg ggtcggcgtt gatcagcact
     1441 gcgcccgggt tctcgatcag cagcgtcgcg tcggggacga agtactccag cttctcgcct
     1501 tgctggcgag ccaggatcgc ttcgttctcg tacgagatca acacgtcgcc ggtgccggcc
     1561 aggaacgccg tcgtcgcgtc gcggccgctg ccggggaggg cgacggtgtg ctcgaagaag
     1621 gccgtgaggt agtcggcggc ctgttgctcg gtgccgccgt tggccagtac ctggccgtag
     1681 gcggcgagga tgttccagcg ggcggcaccc gagctaccgg ggttgggtgt cacgatgccg
     1741 atgtcgtcgc ggatcaggtc ggcccatccg gtgatgccct tcgggttgtc gggccgcacg
     1801 acgagcacga cgaccgagtc ggagacgatg ccctgggtcg gcccagcgtt ccagtcggcc
     1861 gcgacctgac cggagtcgac caggcgggtg acgtcgcctt cgaggctgaa gtggacgtag
     1921 tcggctttga ggccggcgac cacggcccgg ctttgttcac cggaggcacc gtacgactcg
     1981 gtccaggtga ccccagcgcc tgcgggtgtc ttggcgaacg cggcctgcat cgcgttgttg
     2041 gcggccttgg gcacggcgaa gccgaccagg ttgaggttgg tcgacgaggt gccgcccccg
     2101 tcccctttgg cgtcgctgct accgcaggcc atgacaccca ccccggccat gacggccatg
     2161 accgcggcag cagtggtgcg ccagcggcgg cgaaccggcg aggtggccgg tgttggggac
     2221 gatgtcgagg gggtcacgag ataactccta aatatgatcg gattagtcgt ctttagggaa
     2281 ccactgtgag acggattcca caacacggag tatcgtgatt ttccgatggg tggcacgcgg
     2341 cgtgtcggcc cgcgggtttg ttcgcagggg tggccggcgc tgggcataat gtggtcgtgg
     2401 cttcgttctc gctccggtcg ctccggcccc tgccgctggg gatccccggc gtcgcgtccg
     2461 ccgatcgcat cgcgtgggca caatgcgccg tgatgggggt ggtcaacgtc acccccgatt
     2521 cgttctccga cgggggactg cacgccgatg tcgacgacgc ggtcgctcat gccaagctgc
     2581 tcgcgacaca gggcgcgctg gtcgtcgacg tcgggggcga gagcacccgc cccggcgcag
     2641 gcgaggtgag cgtgcacgaa gagctggcca gggtggttcc cgtcgtcgag cgcctggctc
     2701 gcgaggcgtc ggtgctgatc agcgtcgaca ctcgcaagcc cgaggtcgcc gcacaggccg
     2761 tcagcgccgg ggcacatctc gtcaacgacg tcggtggcct gcgcgatccg gagatggtcg
     2821 ccgcctgcgc ggacctgggt gtgccggtgg tcgtcgtgca catgcgcggt gagccggccc
     2881 agatgcaggt cgatccgcgc tacgacgacg tggtcgccga ggtcaccgcc cacttgcgcg
     2941 accgcgccgg ccacgcgctc gcagccgggg tggccgacgt catggtcgac cccggtctcg
     3001 ggttcggcaa gaccgtcgag cacaacctgg cgctgctgcg tgccctgccg ctcgcgatcg
     3061 atcaccccgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngc
     3121 gatcccggca gcatcgcgct gcacctgttc gccgcccaac gcggtgcggc gatggtgcgc
     3181 gtccacgccg tcgccgcgca tcacgaagcg ctgctgatcg atgcagccgt gcgtggcgcg
     3241 tcgggcgagc ttcgtgatcg ttgaggcgag cggccgtgac ggctgacgct gggccagagc
     3301 cgttggcgcg cctcgacgac cggcgccgcg ggctggcgtg gcagttcgcc gagccgctcg
     3361 ca
//
LOCUS       DAORNF010000345         3358 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_21335_length_3358_cov_1.822888, whole genome shotgun sequence.
ACCESSION   DAORNF010000345 DAORNF010000000
VERSION     DAORNF010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3358
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21335_length_3358_cov_1.822888"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(95..658)
                     /locus_tag="PLV68_13300"
     CDS             complement(95..658)
                     /locus_tag="PLV68_13300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02273.1"
                     /translation="MSENLRQYTKTVYAFDHVLKSAKPTAFARKAPFPGWTGQNIYEH
                     IIGNLALIKGYATDKPLKAYPKMGKDPLGQWIKMRDETLSALDHAGVLQKITHQPFGA
                     DFGDMPLDVLVGFMAADMVPHVWDMARTAKVDERLEPSMIKFSTAAWKTLPEAVLRGK
                     GMMGAKVASAKGADAQTKFLNYLGRTV"
     gene            760..1143
                     /locus_tag="PLV68_13305"
     CDS             760..1143
                     /locus_tag="PLV68_13305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRE02274.1"
                     /translation="MVVDRARDQPREQPSDPPSDPLSDAEIDRIFRALADATRRDILR
                     RTLIGEVPVSGLAQDYAMSFAAVQKHVAVLEEAGLVSKRVAGRERRVRANPEIIRRAR
                     ALLDQYEQIWRGRIAGLDALFAEEP"
     assembly_gap    1319..1328
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1329..2205
                     /locus_tag="PLV68_13310"
     CDS             <1329..2205
                     /locus_tag="PLV68_13310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HRE02275.1"
                     /translation="QIERFWGPVEYPARFTRHDMYVGGESHYVMTGPGGDQSRGWWEF
                     LEVVEGERFTVLDGFANPDGTKSDFAPPMRMDFVFSSTPGGSRMTTTTLFASIETLNQ
                     MLEMGMEAGMQSAMSQIDAIVADLASFAAGVAADADIISDTQVRVARVIRGTVDQVWR
                     AHHEPDLVARWLTGPDGWIMSRCDLATEVGGEYRYEWAPAPGTEGETFGFGGELVASE
                     PPHRAVTTERMLGIDGPTTLNEMTLTAVDGGTLCSILITYPDAETRDMILGTGMVFGM
                     EASYARLEREVLATA"
     gene            complement(2096..>2977)
                     /locus_tag="PLV68_13315"
     CDS             complement(2096..>2977)
                     /locus_tag="PLV68_13315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase C-terminal
                     domain-containing protein"
                     /protein_id="HRE02276.1"
                     /translation="GDLVERVERRLGSMRLDEQLRRPSPGEETTTALIERLRQTRLRG
                     LDWSAAATRLRERVRFLRQSAGDTASNTPGSAPSSTASSAGEQAWPDWSDKGLVDTLE
                     TWLAPYLHGITSMADVATLDLTMILRAGLAWPLGQQLDELAPPTLTLASGRSVTVDYS
                     GEQPSAAVRVQDLFGTATHPTVAGRAVLLHLLSPADPDHRRPARLLGRFVGRGAQGDG
                     RSLPQAPMAGRPGHRNAEALEVAGPVAVTRSLRYGRRNTVTPWRGPRAPTGRSWPPCR
                     RPCRCRGSCRGSRHPGR"
     assembly_gap    2978..3134
                     /estimated_length=157
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3135..3329)
                     /locus_tag="PLV68_13320"
     CDS             complement(<3135..3329)
                     /locus_tag="PLV68_13320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02277.1"
                     /translation="MLRRAKDIAARARSSFDPDAIDPDRSGPLLVLAYPDRLAARRNQ
                     PGQFQLRTGAGAWIERTDPLA"
BASE COUNT          480 a         1082 c         1102 g          527 t
ORIGIN      
        1 accactgatc acccacgccc cggctcaagc gccccggctc aagcgccccg gctcaagcgc
       61 cccggctcag acgaggcggc gcagacgagg cgggtcagac ggtgcggccg aggtagttca
      121 ggaacttggt ctgggcgtcg gcgcccttgg cgctggccac cttggcgccc atcatcccct
      181 tcccgcgcag cacggcctcg ggcagcgtct tccatgctgc ggtcgagaac ttgatcatgc
      241 tgggctcgag gcgctcgtcg accttggccg tgcgggccat gtcccacacg tgcggcacca
      301 tgtcggcggc catgaacccg acgaggacgt cgaggggcat gtcgccgaag tcggcaccga
      361 agggctggtg ggtgatcttc tgcagcacgc cggcgtgatc gagagcggag agggtctcgt
      421 cgcgcatctt gatccattgc ccgagcgggt ccttgcccat cttggggtag gccttgagcg
      481 gcttgtcggt ggcgtatccc ttgatcagcg ccaggttgcc gatgatgtgc tcgtagatgt
      541 tttgccccgt ccagccgggg aacggcgcct tgcgggcaaa ggctgtgggt ttggccgact
      601 tcaacacgtg gtcgaaggcg tagacggtct tggtgtactg acgcaggttc tcgctcatgg
      661 accgaactgt agttcccgtc tcgacccctg ccgtccggtg ccgtccgctg gggacggagg
      721 cgttgacggc gtacgggtcg ctcgttaatc tacaaccata tggttgtaga tcgagcacgt
      781 gaccaacccc gcgaacaacc gagcgaccca ccgagcgacc cactgagcga tgcggagatc
      841 gaccggatct tccgggcatt ggccgacgcc actcgccgcg acatcctgcg ccgcaccctg
      901 atcggtgagg tgccggtatc gggtctggcc caggactatg cgatgtcgtt cgccgcggta
      961 cagaagcacg tcgcggtgct ggaagaggct gggttggtca gcaagcgtgt cgccgggcgt
     1021 gaacggcggg tacgagccaa tcccgagatc atccgccgcg cccgtgcctt gctcgaccag
     1081 tacgagcaga tctggcgcgg ccgcatcgcc ggtctcgatg cactgttcgc cgaagagccc
     1141 tgacgagcga ccccgatcac caccgctcaa ttcgcccgtc atccacacat tccctcaaca
     1201 gttccatacg gaaggatcag catcatgccc atcacctctg tcaccaaaga ccctgacgga
     1261 ctcacgatga ccgtcgtcgc cgacttcccg gtgccggtcc ggcgcctgtg ggacgcctnn
     1321 nnnnnnnncc agatcgagcg gttctggggt ccggtcgagt atccggcccg cttcacccgc
     1381 cacgacatgt acgtcggcgg tgagtcgcac tacgtcatga ccggccccgg cggcgaccag
     1441 tcgcgcgggt ggtgggagtt cctcgaagtc gtcgagggcg agcgcttcac cgtgctcgac
     1501 ggcttcgcca accccgacgg caccaagtcc gacttcgctc cgccgatgcg gatggacttc
     1561 gtgttctcgt ccacgcccgg tggttcccgg atgaccacga cgaccctgtt cgcctcgatc
     1621 gagacgctga accagatgct cgagatgggc atggaagcgg gcatgcagtc ggcgatgagc
     1681 caaatcgacg cgatcgtcgc cgacctcgcg tccttcgccg cgggtgtcgc ggccgatgcc
     1741 gacatcatca gcgacaccca ggtgcgcgtg gcgcgggtga tccgcggcac ggtcgaccag
     1801 gtatggcgtg cccaccacga acccgacctc gtcgcccggt ggctgacggg tcccgacgga
     1861 tggatcatga gccgctgtga cctggcgacc gaggtcggcg gcgagtatcg gtacgagtgg
     1921 gcgcccgcgc ccggtacgga gggcgagacg ttcggcttcg gcggcgaact ggtggccagc
     1981 gaaccgccgc accgcgccgt gaccaccgag cggatgctcg gcatcgacgg ccccacgacg
     2041 ctgaacgaga tgaccctcac cgcggtcgac ggcggcacgc tgtgctcgat cctgatcacc
     2101 tacccggatg ccgagacccg cgacatgatc ctcggcaccg gcatggtctt cggcatggag
     2161 gccagctacg cccggttgga gcgcgaggtc ctcgccacgg cgtaaccgtg ttgcggcgac
     2221 cgtatcgcag cgacctggtg acggcgaccg gcccggctac ttcaagcgct tcggcgttgc
     2281 ggtggccggg tcggccggcc attggtgctt ggggtagcga ccggccatct ccttgcgcac
     2341 ctcggcccac gaaccggccc agaagccggg caggtcggcg gtgatctgga tcggcggggg
     2401 agagcaggtg caacagtacg gcccgtccgg ccacggtcgg gtgtgttgcc gtgccgaaca
     2461 ggtcctgcac ccgcaccgcc gcgctcggtt gctcgccgct gtagtcgacg gtcaccgagc
     2521 ggcccgacgc cagcgtcaag gtgggcgggg ccagttcgtc gagctgttgg ccgagcggcc
     2581 acgccagccc ggcgcgcaag atcatcgtca ggtccagtgt ggcgacatcg gccatcgagg
     2641 tgatgccgtg cagatagggc gccagccacg tctccaaggt gtcgaccaga cccttgtccg
     2701 accagtccgg ccaggcctgc tcgcccgcgc tgctggcagt gctgctcggg gcgctgcctg
     2761 gcgtgttgct tgctgtgtca cctgccgact ggcgcaggaa gcggacccgc tcgcgcagcc
     2821 gggttgccgc ggccgaccag tcgaggccgc gcaggcgggt ctggcgcagg cgctcgatca
     2881 gggcagtggt cgtctcctcg ccgggggagg ggcggcgcag ctgttcgtcc aggcgcatgc
     2941 tgccgagtcg gcgctcgacc cgctcgacca gatcgccnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnncgcgag tgggtcggtg cgttcgatcc acgcgcccgc cccggtgcgc
     3181 aactggaact gtccgggctg gttgcgccgt gctgcgagcc ggtccgggta ggccagcacc
     3241 aacaatggcc cgctgcggtc ggggtcgatc gcgtccgggt cgaagctcga ccgggcgcgg
     3301 gcggcgatgt ccttcgcgcg gcgcagcaag cggtcgatcg cgccgcggtc ggcctgcg
//
LOCUS       DAORNF010000346         3358 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_21338_length_3358_cov_1.452619, whole genome shotgun sequence.
ACCESSION   DAORNF010000346 DAORNF010000000
VERSION     DAORNF010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3358
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21338_length_3358_cov_1.452619"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>369)
                     /locus_tag="PLV68_13325"
     CDS             complement(<1..>369)
                     /locus_tag="PLV68_13325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02278.1"
                     /translation="GGVFGLVADAAGSTGNFAASPLAPSASWSAGGNSGGFSWSYPLV
                     MPASPGGLGPSLGVSYSSQAVEGINSDVAAQAGVMGLGWDLAGGGYIERRYVACTDPA
                     IGGAVRANCWAGYNASIVLGG"
     assembly_gap    370..503
                     /estimated_length=134
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<504..2316)
                     /locus_tag="PLV68_13330"
     CDS             complement(<504..2316)
                     /locus_tag="PLV68_13330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02279.1"
                     /translation="MSTRERMRASGHAPRRTLAVLLAWAVVAASLVFASPAAAVGGPV
                     LSGFVPVAPARLLDTRPGGTTIDAFGSGSGAVGASASTTVRVAGRGGVPSAGVGAVAL
                     NVTSVDATHDTYVSVFAAGTPRPASSNLNPSAGGAPTPNMVVVPLSAEGDVELYNAAG
                     SVQLVVDVAGWFPVGSAYTPVTPRRLLDTRNPTASWDGQPGGQVVGGATFDLRVTGTA
                     DVPSSGVGAVLLNVTAVDATLPSFVTVFAHDGQRPVASSSNPAPGRVTPALVAAPVGS
                     DGKVSLYNSAGATHLVVDVVGWIAAGAPYVPLIPARLLDTRAGEPTVDERAAGGGRVG
                     GASVDHKAINLHVAGRAGIPVGVETVVINVTAVNATAPSYVTVWPAGTDRPWASNLNT
                     VPGLISTNLVVARVSAAGDIALFNSAGMVDLIVDVVGYFMAPASTDPPDPDPGPGPDP
                     VATPLDNIWLDSTSAPIEQDVPSPTPTYVLPTPGVATVTAATGLTQAGTLPVFVRSDD
                     PAAVPFGVRVEVLSQAAADAAGAEVLLVQITPTVVPAHDVALSVELDYSAFADRFGGD
                     WADRLGVVAFDGCAVSAPGPDCDGDEWVPSVVDHERDR"
     assembly_gap    2349..2394
                     /estimated_length=46
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2395..>3358)
                     /locus_tag="PLV68_13335"
     CDS             complement(<2395..>3358)
                     /locus_tag="PLV68_13335"
                     /inference="COORDINATES: protein motif:HMM:NF012632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02280.1"
                     /translation="CWGSNDHGQLGNAGVSAAGINVPVAVEGVADAVAVSTGNRHSCA
                     LTVIGAVWCWGFNAAGQLGNGDPTLTDSVVPVRVVVPPAQSVAAGGEHTCAVSSAGAV
                     WCWGNNNSGELGDGTLINSARPVVVGADDALSGIAAVAVGGETSCAVGTDGRAWCWGA
                     NDSGQLGNGDPVGDVRRTPQVVPDVAGATAVAVGARHACAVTAAGVSCWGANGSGQTG
                     TGTFSSAERSAHLVARTEGSVTVSAGARHTCAVLRDGTVWCWGDDTFGQLGNDAASTA
                     SQPLPGAVIGVTSAVAVSAGAGHSCASRAPEAGGGTWCWGSNDSG"
BASE COUNT          547 a         1208 c         1009 g          414 t
ORIGIN      
        1 cccgccgagc acgatcgagg cgttgtaacc agcccaacag ttggcgcgca ccgccccgcc
       61 gatcgccgga tcggtacacg ccacgtagcg gcgctcgatg tagccaccac cggccaggtc
      121 ccatccgagg cccatcaccc ccgcctgcgc ggcgacgtcg gagttgatgc cctcgaccgc
      181 ctgcgacgag tagctcaccc ccaaggacgg gccgaggcca ccgggcgatg ccggcatcac
      241 caacgggtac gaccacgaga acccgccgct gttgccaccc gccgaccatg atgccgacgg
      301 cgcgagcggc gacgcggcga agttgccggt cgatcctgct gcgtcggcaa ccaacccgaa
      361 cactcctccn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      481 nnnnnnnnnn nnnnnnnnnn nnncgcgatc gcgctcgtgg tcgacgaccg acggaaccca
      541 ctcgtcaccg tcgcagtccg gccccggcgc gctcacagca catccgtcga acgcgaccac
      601 accgagacgg tcggcccaat caccgccgaa ccgatctgcg aatgccgagt agtcgagctc
      661 gaccgacagc gcgacgtcgt gcgccggcac cacggtgggt gtgatctgca ccagcaacac
      721 ctccgccccg gcagcgtcgg cagccgcttg cgagagcacc tcgacccgca cgccgaacgg
      781 caccgcggct gggtcgtctg agcggacgaa cacggggagg gtacccgcct gggtgagacc
      841 ggttgcggcc gtgacggtcg cgacgccggg ggtcggcagt acgtacgtcg gtgtcggtga
      901 cggaacgtcc tgctcgatgg gtgccgaggt ggagtcgagc cagatgttgt cgagtggtgt
      961 cgccaccgga tcaggacctg ggccgggatc ggggtctggc ggatcggtgg acgcgggcgc
     1021 catgaagtag ccgaccacgt cgacgatcag atcgaccatg cccgcactgt tgaacaaggc
     1081 gatatcgcct gcagcggata ctcgggcgac cacgaggttg gtcgagatga ggccgggcac
     1141 cgtgttgaga ttcgatgccc acggccggtc ggtaccagcg ggccagaccg tcacgtacga
     1201 cggcgcggtc gcgttgacgg ccgtcacgtt gatcacgacg gtctcgaccc ccaccggaat
     1261 gcccgcccgg ccagcgacgt gcagattgat cgccttgtga tcgacgctcg caccaccgac
     1321 ccggccacca cccgcggccc gctcgtcgac cgtcggttca cccgcgcgtg tgtccaacaa
     1381 ccgcgccggg atcagcggca catacggcgc tcccgctgcg atccaaccga ccacatcgac
     1441 cacgaggtgg gttgcgccgg ccgagttgta caacgagacc tttccgtccg acccgaccgg
     1501 ggccgccacc aacgcggggg tgacgcggcc aggcgccggg tttgacgacg acgcgaccgg
     1561 acgttgcccg tcatgcgcga acaccgtgac gaagctgggc agcgtcgcgt cgactgccgt
     1621 cacgttcagc aacaccgcgc cgacaccgct tgacggcaca tcggcggtgc cggtcacccg
     1681 caggtcgaac gtcgcaccac cgacaacctg gcctccgggt tgaccgtccc acgaagcggt
     1741 cgggttgcgc gtgtcgagta gccgcctcgg cgtgactggg gtgtaggccg acccaaccgg
     1801 gaaccacccc gccacgtcaa ccacgagctg cacgctgcct gccgcgttgt acagctcgac
     1861 gtcgccctcg gccgacaatg gcaccacgac catgttcggg gtcggcgcgc caccggccga
     1921 cgggttgagg ttcgatgacg ctggccgcgg cgtgcctgcg gcgaacaccg acacgtaggt
     1981 gtcgtgagtc gcgtcgacgg aggtcacgtt caacgcgacc gcgccgacgc ctgccgaggg
     2041 cacgccgccg cgtccggcga cacgcaccgt cgtcgatgca ctcgcaccca ccgcaccaga
     2101 gcctgagccg aacgcgtcga tggtcgtgcc gcctgggcgt gtatccaaca gacgcgccgg
     2161 agcgaccggc acgaacccgc tcagaacagg gccgccaaca gccgcagcag gcgacgcgaa
     2221 caccagcgat gccgcaacga ctgcccacgc caacagcacc gccaacgtcc gccttggcgc
     2281 gtgcccgctc gcccgcattc gttcccgtgt gctcatgacg caacctccac cggaaccggc
     2341 tgggcggcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngcccga
     2401 gtcgttcgaa ccccagcacc atgtgccgcc tccggcctct ggagcgcggg acgcacagga
     2461 atgacccgca ccggcgctga ccgccaccgc agacgtgacg ccgatgacag cacctggcaa
     2521 cggctgtgat gcggtgctcg cggcatcgtt gccgagctga ccgaacgtgt cgtcgcccca
     2581 acaccacacg gtgccgtcgc gcaagaccgc gcacgtgtgc cgcgctccag ccgacacggt
     2641 gaccgaaccc tccgtacgtg ccacgaggtg cgccgatcgc tcagccgacg agaaggtgcc
     2701 ggtgcccgtc tgaccggagc cgttcgctcc ccaacacgag actccggccg cggtcaccgc
     2761 gcatgcgtgt cgtgctccca ccgccacggc agtggccccg gccacatcgg gcaccacttg
     2821 tggcgtccga cgcacgtcgc cgacgggatc accgttgccc agttggcccg agtcgttcgc
     2881 accccagcac cacgcgcggc cgtccgttcc gactgcacac gacgtctcgc cgccaaccgc
     2941 aaccgcagca atcccgctga gggcatcatc ggcgccgacg acgactggcc gcgccgagtt
     3001 gatcagcgtg ccgtccccca actcacccga gttgttgttg ccccaacacc acaccgcgcc
     3061 cgccgacgac accgcgcatg tgtgctcacc acccgccgcg accgactgcg ccggtggcac
     3121 caccacccgc accggtacga ccgaatcggt caaggtcgga tcaccgttgc ccaactgccc
     3181 agcggcgttg aagccccagc accacaccgc tccgatcacc gtcagcgcgc acgagtggcg
     3241 gttcccggtg gagacggcga ccgcgtcggc gactccttcg acagcgaccg gcacgttgat
     3301 ccccgctgcc gagacgcccg cgttgccgag ctggccgtgg tcgttcgaac cccagcag
//
LOCUS       DAORNF010000347         3353 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_21391_length_3353_cov_1.826865, whole genome shotgun sequence.
ACCESSION   DAORNF010000347 DAORNF010000000
VERSION     DAORNF010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3353
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21391_length_3353_cov_1.826865"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(357..>570)
                     /locus_tag="PLV68_13340"
     CDS             complement(357..>570)
                     /locus_tag="PLV68_13340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02281.1"
                     /translation="VPEAAADTVRWSYDHVASPWHVAFTPGIRAIDVTSADGQPLLRN
                     GLPTRVDLGEVRAIAAEQAARLFARL"
     assembly_gap    571..587
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<588..1454)
                     /locus_tag="PLV68_13345"
     CDS             complement(<588..1454)
                     /locus_tag="PLV68_13345"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRE02282.1"
                     /translation="MAAWPDRTLAADETPGLVCAHHHLYSTLARGMPAPPQQAHDFFG
                     ILHQIWWRLDAALDLDMLRASALLGAVEALQCGTTAIVDHHEGPNAIEGSLDVIADAC
                     AEVGVRVLCAYGVTDRWQDDATLWPDDATPPPAMTRAARAGLDECIRFIRGGGRGMVG
                     IHAAFTCSDDTLAAAADAARDLGVGVHVHVAEGPGDVSAARRLTGMADDDWLLVHGVH
                     LDRDVAGRLAHNPRSNMNNAVGYARPATRPNTVVLGTDGIGADMLEEFRLAYVAHRAD
                     DVTASPETAWSWL"
     gene            complement(1468..2181)
                     /locus_tag="PLV68_13350"
     CDS             complement(1468..2181)
                     /locus_tag="PLV68_13350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase N-terminal domain-containing
                     protein"
                     /protein_id="HRE02283.1"
                     /translation="MSEPPSTPLVPADFTRDLLRWSNALAGVARTGLGFTQSLYERER
                     FEEVLHIAADIKAAADDALEVRREQDHFVQEWMESVGEGVPGYVTPKVAVGAIVANDQ
                     GEILLVQRADSLVWLYPTGWADSGYSASEVVVKEVREEAGIECEPVHLLAVVDGQRMG
                     FSRFAMYMMVFFCRATGGSLTPHPLETADVGWFGRDALPPRTAGADWWSPWAFAAVDG
                     DLGTTLFDPPRAPVWRGEQ"
     gene            complement(2193..>3064)
                     /locus_tag="PLV68_13355"
     CDS             complement(2193..>3064)
                     /locus_tag="PLV68_13355"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HRE02284.1"
                     /translation="AAILAEPATRSRHVAAILQFAQANGFAGIDIDYEIFAFGDPRAT
                     WTTTRPNWVAFVIELAAALHGVGKTLTVSVPPQYDPERTGGDRGYWVYDYEAIGPVVD
                     FIRIMAYDYSTSSAGPIAPLDWVEGLVRDAAAMVEPHKLILGVPVYGYNWPVSTLGVC
                     PPDAAPTRRNQSAKSALALAAERGVTVRFDDAVAEANFDYNDHLSGVDAAGAATACTV
                     TRRVWFSNAQAVFERAWIASRHQLAGIGIWSLGSDEPAVWQAIDAVRAGDEQWLAAGA
                     TTGSTAGSATGSG"
     assembly_gap    3065..3124
                     /estimated_length=60
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3125..3306)
                     /locus_tag="PLV68_13360"
     CDS             complement(<3125..3306)
                     /locus_tag="PLV68_13360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02285.1"
                     /translation="MHAWAPYWQTDNAFASFEPNTGVFSDLSLFAYHATSADSITAYS
                     GLRDGVVDTYRAAATAA"
BASE COUNT          477 a         1145 c         1169 g          485 t
ORIGIN      
        1 ggttggtcgc cgtgctcgtc gcatgaacca ccacccccgg ccggagacta cgaaccacgc
       61 cgaagcgagc gagcacccgg acacaccgct gtccgggtgt cgccgcggcg tcactggcct
      121 gggctacctc aaccgacgcc cgtggctgag cccgatcacc cgagccgggc tgacgggttt
      181 gccatctggt gaatcggcat ggtgtgcccg gcgctgacac cgacgacccg gccgcctgac
      241 ctgctggcgc ctcgcccgca tccccgagtt atggccggcg aagtcacgtg gtgaatcgtt
      301 gccgccgggt cggggttggc ggccggcatc gtgctcgtcg gcgaccattc gccctgctag
      361 agacgggcga agaggcgggc tgcttgttcg gcggcgatcg cgcggacttc tccgaggtcg
      421 acacgggttg ggaggccgtt gcgcaggagg ggttggccgt cggcgctggt gacgtcgatc
      481 gcgcggatgc cgggggtgaa ggcgacgtgc cacggcgagg cgacatggtc gtagctccac
      541 cgcacggtgt cggcggcggc ctcgggcacc nnnnnnnnnn nnnnnnngag ccacgaccat
      601 gcggtctcgg gtgacgcggt gacgtcgtcg gcccgatggg ccacgtaggc caggcggaac
      661 tcttcgagca tgtccgcgcc gatgccgtcg gtgccgagca ccacggtgtt cggccgcgtc
      721 gcggggcggg cgtagccgac cgcgttgttc atgttgctgc gcgggttgtg ggcgaggcgg
      781 ccggccacgt cgcgatcgag gtgcacgccg tggaccagca gccaatcgtc gtcggccatc
      841 ccggtcaaac gccgtgcggc ggagacgtcg ccaggtcctt cggcgacgtg gacgtgcacg
      901 ccgacgccga ggtcacgggc cgcatcggcg gcagcggcaa gcgtgtcgtc gctgcacgtg
      961 aacgcggcgt ggatgccgac catgcctcgt ccgccgccgc ggatgaaccg gatgcactcg
     1021 tcgagcccgg cgcgagcggc gcgcgtcatc gccggtggcg gtgtcgcatc gtcgggccac
     1081 agcgtggcgt cgtcctgcca acggtcggtg acgccgtagg cgcacagcac gcgcacgcca
     1141 acttcggcgc acgcgtcggc gatcacatcg aggctgccct cgatcgcgtt ggggccttcg
     1201 tgatggtcga cgatcgcggt ggtgccgcac tgcagcgcct cgaccgcgcc cagcaacgcg
     1261 ctggcgcgca gcatgtcgag atcgagcgcc gcgtcgagcc gccaccagat ctggtggagg
     1321 atgccgaaga agtcgtgtgc ctgttgcggc ggagcgggca tgccccgcgc cagcgtcgag
     1381 tacaggtggt ggtgggcgca caccaacccc ggcgtctcgt cggccgcgag ggttcgatcc
     1441 ggccaggccg ccaccgacgt gtgccctcta ctgctcgccg cgccacaccg gggcgcgggg
     1501 cgggtcgaac agggtcgtgc ccaagtcgcc gtcgacggca gcgaaggccc acggcgacca
     1561 ccagtcggcc ccggcggtgc gcggcggcag cgcatcgcgg ccgaaccagc cgacgtcggc
     1621 ggtctccaac gggtgcggcg tcaacgaacc gcccgtggcc cggcagaaga acaccatcat
     1681 gtacatcgcg aaacgggaga agcccatccg ttggccgtcg accacggcca acaggtgcac
     1741 cggctcgcac tcgatgccgg cctcttcgcg cacctccttg accacgacct cggacgccga
     1801 gtagccgctg tcggcccacc cggtggggta cagccacacc aggctgtcgg cccgctgcac
     1861 cagcaggatc tcgccctggt cgttggcaac gatggccccc acggcaacct tcggcgtgac
     1921 gtagccaggc accccttcac cgacgctttc catccactcc tgcacgaagt ggtcttgctc
     1981 gcggcgcacc tcgagcgcgt cgtcggccgc ggccttgatg tcggcggcga tgtgcaacac
     2041 ctcttcgaac cgctcgcgct cgtacaggct ctgggtgaag cccagcccgg tgcgggcaac
     2101 gcccgcgaga gcgttcgacc agcggaggag gtcgcgggtg aagtcggccg ggacgagcgg
     2161 tgtggagggc ggttcactca tcgctgccga ccctatccgg agcctgtcgc cgagccagcc
     2221 gtcgacccgg tcgtcgcccc agccgccagc cactgctcat caccggcacg cacggcgtcg
     2281 atcgcctgcc acaccgcagg ctcgtcgctg cccagcgacc agatgccgat tccggccaac
     2341 tggtggcgcg acgcgatcca cgcccgctcg aacaccgcct gcgcgttgga gaaccagact
     2401 cgccgggtga cggtgcaggc ggttgcggcc ccggccgcat ccacaccgct gaggtggtcg
     2461 ttgtagtcga agttggcctc ggccaccgcg tcgtcgaagc gcaccgtcac accgcgttcg
     2521 gcggccagcg cgagcgctga cttggcggac tggttgcggc gcgtcggggc ggcgtctggc
     2581 gggcacacgc ccagcgtcga caccggccag ttgtagccgt ataccggcac gccgaggatc
     2641 agcttgtgcg gttcgaccat ggctgccgcg tcgcgcacca ggccttccac ccagtcgagg
     2701 ggcgcgatcg gccccgcgct ggaggtgctg tagtcgtagg ccatgatgcg gatgaagtcg
     2761 accaccggtc cgatcgcttc gtagtcgtag acccagtagc cacggtcgcc gcccgtgcgc
     2821 tcggggtcgt actgcggtgg cacgctgacc gtcaaggtct tgcccacacc gtgcagcgcg
     2881 gcggcgagtt cgatcacgaa cgcgacccag ttcggccggg tggtcgtcca ggtcgcgcgc
     2941 gggtcgccga aggcgaagat ctcgtagtcg atgtcgatcc cggcgaaacc gttggcctgg
     3001 gcgaactgga ggatcgcggc cacgtggcgg ctgcgcgtcg cgggctcggc caagatcgcc
     3061 gccannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnngccgcg gttgcggcgg cgcggtaggt gtcgaccacc ccgtcacgca gcccgctgta
     3181 cgccgtgatc gaatcggccg acgtcgcgtg gtacgcgaac agcgacagat cgctgaacac
     3241 ccccgtgttg ggctcgaacg aggcgaacgc attgtcggtc tgccagtacg gagcccaggc
     3301 atgcacggtg atcggcggtc cgactcgacg ccaccgggtc gtcgtctcgc ggt
//
LOCUS       DAORNF010000348         3352 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_21402_length_3352_cov_1.477707, whole genome shotgun sequence.
ACCESSION   DAORNF010000348 DAORNF010000000
VERSION     DAORNF010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3352
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21402_length_3352_cov_1.477707"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            316..459
                     /locus_tag="PLV68_13365"
     CDS             316..459
                     /locus_tag="PLV68_13365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02286.1"
                     /translation="MREDTDSIAEQLRAEAARIRREEAKRLAVIDAANEAADAVLDDD
                     SPA"
     gene            534..1310
                     /locus_tag="PLV68_13370"
     CDS             534..1310
                     /locus_tag="PLV68_13370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRE02287.1"
                     /translation="MSDIVQYEVQGRVAVITLNRPEARNAVNGDVANGMEAAIDQLEG
                     DPAVWVGVLRANSEGQAKPVFCAGADLKAINSGQAGALSTRRGGFAGFVYRERTKPVI
                     VAVDGLATAGGLEIVLGADLVVASTQASFGLAEVKRNLIAGAGGLFRAIGQAAAMEAI
                     LTGEPFTAERAYELGLVSRLTEPGRAFEEAMNLAAQITAAAPLAVYASRRVVLAANSG
                     DDEMLKDMTNREFAAVMASEDTKEGLTAFIEKRAPNWQGR"
     gene            complement(1409..1552)
                     /locus_tag="PLV68_13375"
                     /pseudo
     CDS             complement(1409..1552)
                     /locus_tag="PLV68_13375"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     assembly_gap    1704..1744
                     /estimated_length=41
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1785..2381)
                     /locus_tag="PLV68_13380"
     CDS             complement(1785..2381)
                     /locus_tag="PLV68_13380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02288.1"
                     /translation="MARSGKSDPNPLQRMVDLAVFAPIGLAIAARSDLPRWAKLGRRQ
                     LATQLHTVNHLAKVVIGQGRSELTRRLDAAEAARTAPDPHIPDPPDSHIDVRHAPAQP
                     VPSRRVPSRPVEALPSEPVSPVAVVPRGTRAAANLQAQSAEPMPNPIDGYDSLAASQV
                     VARLNTLSADELAQVKDYEAAHRSRQTILAKVDQLLQD"
     gene            complement(2415..>2600)
                     /locus_tag="PLV68_13385"
     CDS             complement(2415..>2600)
                     /locus_tag="PLV68_13385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02289.1"
                     /translation="VAADDEDDEDEDEPEDKSEAGDRLQPKRSDEQLCPSCFLLVRNS
                     APTCPIGDDDCPIVVAR"
     assembly_gap    2601..2645
                     /estimated_length=45
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2646..2884)
                     /locus_tag="PLV68_13390"
     CDS             complement(<2646..2884)
                     /locus_tag="PLV68_13390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02290.1"
                     /translation="MADDDMLDPEDEQVEDEATEAAPEEIDEDAIDGEGDADFVDIDE
                     EFGGDDDDTGDDDDEDDVGETSGPVRKSVAASDDD"
     assembly_gap    2989..3051
                     /estimated_length=63
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3052..>3352)
                     /locus_tag="PLV68_13395"
     CDS             complement(<3052..>3352)
                     /locus_tag="PLV68_13395"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HRE02291.1"
                     /translation="GMTGVFDDAMSRSAALYDALRHDFPHQASYAVAMAYRLRYSMQF
                     NAREAMHMLELRSSPQGHPAYRRVAQEMHRLIADKAGHRAIAAAMSHMTDEAPELE"
BASE COUNT          479 a         1039 c         1134 g          551 t
ORIGIN      
        1 cgcgtcggta tgtatggata cgggttccgg gcgagcaaac ggtgccatct cctgtggtct
       61 ttgaggttag tgagatggaa accgtagacg gaaagctccg agtagtgatt gatggtgaat
      121 tgtgcccgga agactgacac tccgctcggt gacctgggct ggttcgggcg gtgattgctg
      181 ctggaagcgt tggctgcgtg gcggggtgga cgggtggcga gatcgttggt gtggagaacc
      241 actgcccgtg ggaagaaggt gacgacccaa cctagccctg gcgaggggtg acaggggtgg
      301 ggcagactgt cgggtatgag ggaggacacg gactccattg ctgagcagct gcgggcggag
      361 gcggcgcgga ttcggcgtga ggaggcgaag cggttggcgg tgatcgacgc ggccaacgag
      421 gcggctgacg cggtgctcga cgacgattcg cctgcctgac gcgcctgccc aacccacgga
      481 cgtgtccgta gcgggttcgt cgcgacgcgg cacggtttcc tagattctgg gctatgtcag
      541 acatcgtcca gtacgaagtg cagggccgcg tcgcggtcat cacgctcaac cgccccgagg
      601 cccgcaacgc cgtcaacggc gacgtcgcaa acggcatgga agccgccatc gatcaactcg
      661 aaggcgaccc agccgtgtgg gtgggcgtgt tgcgcgccaa cagcgaaggc caggccaagc
      721 cggtgttctg cgccggggcc gacctgaagg cgatcaactc cggccaggcc ggtgcgctca
      781 gcaccaggcg cggcggcttc gcgggcttcg tctaccgcga gcgcaccaag ccggtcatcg
      841 tcgccgtcga cggtctcgcc accgctggtg ggttggagat cgtgctcggt gccgacctgg
      901 tggttgccag cacccaggcc tcgttcggtt tggccgaggt gaagcgcaac ctgatcgccg
      961 gtgccggcgg cctgttccgc gcgatcggcc aggcggcggc gatggaggcg atcctcaccg
     1021 gtgagccgtt caccgccgag cgcgcctacg agctcggcct cgtctcgcgt ttgaccgagc
     1081 ccggtcgcgc cttcgaagag gcgatgaacc tggccgccca gatcaccgcc gccgctcccc
     1141 tggccgtcta cgccagccgc cgggtggtgc tcgccgccaa ctcgggcgac gacgagatgc
     1201 tgaaggacat gaccaaccgt gagttcgccg ccgtgatggc gtcggaggac accaaggaag
     1261 gcctgaccgc gttcatcgaa aagcgcgccc ccaactggca gggccgctga cgaccagcgc
     1321 tgtagaccgt gatcgaacgg gccgtcagac cggcccgttc gaacaggttc ttggtctccc
     1381 gatcgccggg gagcgccgtg gcttcgatgc cgatcaggcc gcgcccgagc gtcgcctcga
     1441 ccgctgcttc gaccagtccg tcgccgaggc ccaactcgcg ggcctctgct tcgacgtaga
     1501 ccagttcgac cacggcccgc tcggcgccac ggtcggcccg ggcgatcaac accccgacga
     1561 ccacatcgtc gatcagcgcc gagaacacat cggtgtcggg gtcggtcagg gccgcggccc
     1621 agtcgatcgg gggacgctcg gccagcgcca acgctccccc gcgggcctcg aaaatgccgt
     1681 cgcgggcaac ctgctcgatc cggnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnncgactt cgtccatcag cccaactcca tccgtccgcg tccgtcagtc ctggagcagt
     1801 tggtcgacct tggccaagat ggtctgccgc gaccgatgcg cggcctcgta atccttgacc
     1861 tgggccagtt catcggcgct cagcgtgttg agccgggcga ccacctgtga cgcggcgagg
     1921 ctgtcgtagc cgtcgatcgg gttgggcatc ggctcggcac tctgcgcctg gagattggcc
     1981 gcagcgcggg taccgcgcgg gaccaccgcg acgggtgaga ccggctcgga gggcaacgcc
     2041 tcgaccggcc gggatgggac cctacgggat gggacaggct gggccggggc gtgacggacg
     2101 tcgatgtgcg agtccggcgg gtccgggatg tgcgggtccg gggcggtgcg cgcggcttcg
     2161 gcggcgtcga ggcggcgggt cagttcagag cgcccctgcc cgatgaccac cttggccaga
     2221 tggttgaccg tgtgcagttg cgtggcgagt tgacgacggc cgagcttggc ccaccggggc
     2281 aggtcggaac gggccgcgat cgccaaaccg atcggcgcga acaccgccag gtcgaccatc
     2341 cgctgcagcg ggttggggtc tgatttgcct gatcgtgcca tgccggatcg tgtcacgccg
     2401 gtggggcggc tgtctcagcg ggcgacgacg atgggacagt cgtcgtcacc gatcgggcag
     2461 gtgggtgccg agttgcgtac cagcaggaag cagctcgggc agagctgctc gtcggaccgc
     2521 ttgggctgga ggcgatcgcc ggcttccgac ttgtcctcgg gctcgtcttc gtcctcgtcg
     2581 tcctcgtcgt cggcggcgac nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnntcatc gtcgtcgctc gcagcgaccg acttgcggac cggtccggat gtctcaccga
     2701 cgtcgtcttc gtcgtcgtca tcgcccgtat cgtcgtcgtc gccgccgaac tcctcgtcga
     2761 tatctacgaa gtcggcgtcg ccttcaccgt cgatggcgtc ttcgtcgatc tcttccggtg
     2821 cggcttcggt ggcctcgtct tcgacctgtt cgtcttccgg atcgagcatg tcgtcgtcag
     2881 ccatcgtgtc tcctcagtcg ttccggtgcg cgggcggcga tcatatgggg gtattccgtc
     2941 agccaacgac cacaccattg tgacaaccgt cacgtagtgc ccgaacccnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngctccaact
     3061 cgggtgcctc gtcggtcatg tgcgacatcg ccgccgcgat cgcgcggtgc ccggccttgt
     3121 cggcgatcag ccggtgcatt tcctgggcga cgcgccggta ggccgggtgg ccctgcggcg
     3181 acgagcggag ctccagcatg tgcattgctt cgcgagcgtt gaactgcatc gaataacgca
     3241 ggcggtacgc catcgccacg gcgtagctgg cctggtgcgg gaagtcgtgg cggagcgcgt
     3301 cgtacagcgc ggccgaacgc gacatcgcgt cgtcgaacac gccggtcatc cc
//
LOCUS       DAORNF010000349         3348 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_21434_length_3348_cov_1.460978, whole genome shotgun sequence.
ACCESSION   DAORNF010000349 DAORNF010000000
VERSION     DAORNF010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3348
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21434_length_3348_cov_1.460978"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="PLV68_13400"
     CDS             <1..228
                     /locus_tag="PLV68_13400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02292.1"
                     /translation="ETRERELAEGHPLFRHVGVVTVAAGSVDELDDACNRVEQAAAQS
                     LIDLRPLAARQAEGWVASLPLGRSVRQGAWL"
     gene            225..>1010
                     /locus_tag="PLV68_13405"
     CDS             225..>1010
                     /locus_tag="PLV68_13405"
                     /inference="COORDINATES: protein motif:HMM:NF014044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HRE02293.1"
                     /translation="MSTAAERGPGWTGRPGVPAEWQRNTMAHLCSVFPFHADTGFGER
                     GVLMGANVTGGMSAFYFDPFEFYSQRHLTNPNMIVMGSVGFGKSATVKALVRRLKAVY
                     GADRYLAIVDPKGEYGPLAADLGLSVIKPYPGGRDRINPMDAGGGDGDSSLLARQGLA
                     VQLIAGVLGRDLTPIEDALLGWAIERRSRTLLGFTLRDLCAEVSDPPDELVQLSRHSP
                     HELARATAPVAFALDKLCARTLRGMFEAGALPYYLHQLDRVAGT"
     assembly_gap    1011..1029
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1030..1517
                     /locus_tag="PLV68_13410"
     CDS             <1030..1517
                     /locus_tag="PLV68_13410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02294.1"
                     /translation="SALVIAAATHWLSGALRGRPQRRSVQVIDEAWAAVRHGAGYFQS
                     SLKLSRAHGVATVLVCHRPSDLTAQSDDGTAANKIAAGLLSDIQTRVLLRQPPEQIPA
                     AAEMFDLTERERGWLGQLVPGRAIWQIGARSAVVQTILAGNERDLFDTDHAMVNDDRA
                     A"
     gene            1501..>2130
                     /locus_tag="PLV68_13415"
     CDS             1501..>2130
                     /locus_tag="PLV68_13415"
                     /inference="COORDINATES: protein motif:HMM:NF013930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHC2 zinc finger domain-containing protein"
                     /protein_id="HRE02295.1"
                     /translation="MTGQHEHRWSIDDVLDRTDLAALLDELTEPAGRTMGPGRKWHCP
                     APYHEDHRASVTMFRDRSGHERWRCWSGDHRGDAIDLVTLTSGRDRADAVEWLANRAG
                     MHPDRPLPPARPKTSPAPPAARVMDPIVARYVHACHRILDASAGLPVREWLHARGIDD
                     HTITANLIGADPGRTMMSRARGLPYGAQIAATFPVYGPGGNLTYVQARYL"
     assembly_gap    2131..2140
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2141..>2419
                     /locus_tag="PLV68_13420"
     CDS             <2141..>2419
                     /locus_tag="PLV68_13420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02296.1"
                     /translation="GAGRKYDNPAAALAPHPRLAFPIPTHDLRAPVTLVCEGMPDALT
                     AAQGGFDAVGLLGAQTPDESVAARLANHADNHDTRLVIVCDPDAAGRRV"
     assembly_gap    2420..2517
                     /estimated_length=98
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2787..2812
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2813..3039
                     /locus_tag="PLV68_13425"
     CDS             <2813..3039
                     /locus_tag="PLV68_13425"
                     /inference="COORDINATES: protein motif:HMM:NF012651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HRE02297.1"
                     /translation="YLTIQTWGHLAGRCAQHLTTGRHVAISGAMRHDQWITRAGERAD
                     RWYARATDVTFLDTPADLSGDDEPSEGSDR"
     gene            3036..>3348
                     /locus_tag="PLV68_13430"
     CDS             3036..>3348
                     /locus_tag="PLV68_13430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02298.1"
                     /translation="MTAAEFVFRHAGGCLIDTPDLEPARPGDTEHWTWVATIWQDRHR
                     PDGWNALEWAPAERGWVLPATTSIGDVVEFGAGAVNPYGNTRFDRWWGWIRRVSYRAL
                     IV"
BASE COUNT          494 a         1226 c         1050 g          425 t
ORIGIN      
        1 gagacccgcg aacgcgaact cgccgaaggg catccgctgt tccgccacgt cggtgtcgtc
       61 accgtcgcgg ccgggtcggt cgacgagctc gacgacgcct gcaaccgagt cgagcaggcc
      121 gccgcccaat cgctgatcga cctgcggccg ctcgccgccc gccaagcaga gggctgggtg
      181 gcgtcactgc cactcggccg ctcggtccgc caaggggcgt ggctatgagc acggcagccg
      241 aacgcggacc gggctggacc ggacggccgg gagtgccagc cgagtggcag cgcaacacga
      301 tggcgcacct gtgctcggtg ttcccgttcc acgccgacac cggcttcggc gagcgcggcg
      361 tgctgatggg agcgaacgtc accggcggga tgtcggcgtt ctacttcgac ccgttcgagt
      421 tctactccca acgccacttg acgaacccga acatgatcgt gatgggctcg gtcggcttcg
      481 gcaagtcggc caccgtcaaa gcactggtcc gccgcctcaa ggccgtctac ggggccgacc
      541 ggtatctggc gatcgtcgac ccgaaaggcg agtacggccc gctcgccgcc gatctcggcc
      601 tgagtgtgat caagccgtac ccgggcggcc gggaccggat caacccgatg gacgccggcg
      661 gcggggacgg cgacagttca ttgctcgccc gccagggcct cgccgtgcag ctgatcgccg
      721 gagtgctcgg tcgtgacctg acccctatcg aagacgcgtt gctcggctgg gcgatcgaac
      781 ggcgcagccg caccctgctc ggcttcacgc tgcgagacct gtgcgccgag gtgtcggacc
      841 ctcccgacga actcgtccaa ctctcgcgcc actcgccaca cgagttggcc cgggcgacgg
      901 caccggtcgc gttcgcgctc gacaagctgt gcgctcgcac cctgcgcggc atgttcgagg
      961 ccggcgcgct gccgtactac ctgcatcagc tcgaccgggt ggccggcacg nnnnnnnnnn
     1021 nnnnnnnnnt cagcgcgttg gtgatcgccg cggcgacgca ctggctgtcg ggcgccttgc
     1081 ggggcaggcc gcagcggcgt tcggtgcagg tgatcgacga ggcgtgggcg gcggtccgcc
     1141 acggcgccgg ctacttccag tcgtcgctca agctgtcccg ggcccacggt gtggcgaccg
     1201 tgctcgtgtg ccaccggccc tccgacctca ccgcccagtc cgacgacgga acagcggcga
     1261 acaagatcgc cgccgggctg ctgtcggaca tccaaacccg ggtgctgctg cgacagcccc
     1321 ccgaacagat ccctgccgcg gccgagatgt tcgacctcac cgaacgcgaa cgcggctggc
     1381 tcggacagct cgtcccgggc cgggcgatct ggcagatcgg cgcccgctcc gcggtggtgc
     1441 agacaatcct cgccggcaac gagcgcgacc tgttcgacac cgaccacgcg atggtgaacg
     1501 atgaccgggc agcatgagca tcgctggtcg atcgacgacg tgctcgaccg caccgacctc
     1561 gccgcgctgc tcgacgagct gaccgaaccg gccggccgca cgatggggcc gggtcgcaag
     1621 tggcactgcc cggccccgta tcacgaggac caccgggcgt cggtgacgat gttccgtgac
     1681 cgcagtggcc acgaacgatg gcgctgctgg tcgggtgacc accgcggcga cgcgatcgat
     1741 ctcgtcaccc tcaccagtgg ccgcgaccgt gccgacgcag tcgagtggct cgccaaccgg
     1801 gccgggatgc accccgaccg gccgctgccg ccggcacgac ccaagacatc acccgcgccg
     1861 cccgcggcgc gggtgatgga cccgatcgtc gcccgctacg tgcacgcctg ccaccggatc
     1921 ctcgacgcct cggcggggct gccggtgcgc gaatggttgc atgcccgggg catcgacgac
     1981 cacacgatca ccgccaacct gatcggcgcc gaccccggcc ggacgatgat gagccgcgcc
     2041 cgcggcctcc cgtacggcgc gcagatcgcg gcgacattcc ccgtgtacgg gccggggggc
     2101 aacctcacct atgtccaggc ccgctacctc nnnnnnnnnn ggcgccggcc gcaagtacga
     2161 caaccccgcc gccgcgctcg ccccacaccc ccggctggcg ttcccgatcc ccacccacga
     2221 cctgcgcgcc ccggtcacgc tcgtgtgcga agggatgccc gacgcgctca ccgccgcaca
     2281 gggcggcttc gacgcggtcg ggctgctcgg cgcccaaacc cccgacgaga gcgttgctgc
     2341 ccggctcgcc aaccacgccg acaaccacga cacccgcctc gtcatcgtct gcgaccccga
     2401 cgcggccgga cgccgagtcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnagc
     2521 ccggctgggc cgaccaactc gaccacgatc tgcaccccga gcggcacgtc gtccccgccg
     2581 gccgctcgct ccacgaggtc gacctgtgac tgcgggaagg atgcgacacc ccatcggtac
     2641 actcgcaggc aacggttcga gagcctgccg tgtctgctct ccgttcacac gtcccactcg
     2701 aagggagacg ccatgaacgt cctgaccgtc accggccgcc tcgccgacga cccggtccgc
     2761 cgtgacaccg ccaagggtgt cgtgtgnnnn nnnnnnnnnn nnnnnnnnnn nntctacctc
     2821 accatccaaa cgtgggggca cctcgccggt cgctgcgccc aacacctcac caccggccgc
     2881 cacgtcgcga tcagcggagc gatgcgccac gaccagtgga tcacccgagc cggagaacgc
     2941 gccgaccgct ggtacgcacg cgccaccgac gtcacgttcc tcgacacccc cgccgacctg
     3001 agcggcgacg acgagccgag cgaagggagc gatcgatgac cgccgccgag ttcgtgttcc
     3061 gccacgccgg tggctgtctg atcgacaccc ccgacctcga gcccgcacgc ccgggcgaca
     3121 ccgagcactg gacgtgggtc gccaccatct ggcaggaccg ccaccgcccc gacggatgga
     3181 acgcgctcga atgggcgccc gcggagcgcg gctgggtgct gcccgccacc acctcgatcg
     3241 gtgacgtcgt cgagttcggc gccggcgccg tcaacccgta cggcaacacc cgcttcgacc
     3301 ggtggtgggg ctggatccgc cgcgtcagct accgggccct gatcgtga
//
LOCUS       DAORNF010000350         3343 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_21472_length_3343_cov_1.722932, whole genome shotgun sequence.
ACCESSION   DAORNF010000350 DAORNF010000000
VERSION     DAORNF010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3343
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21472_length_3343_cov_1.722932"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1537
                     /gene="fusA"
                     /locus_tag="PLV68_13435"
     CDS             <1..1537
                     /gene="fusA"
                     /locus_tag="PLV68_13435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HRE02299.1"
                     /translation="MKALIWRDEELGAKWDEVEIPADMQEQADQYRHELLETIATSDD
                     DFMEKYLGGEDFTEADIKRAIRKGTLAFDFVPILCGSSFKNKGVQPMLDAVIDFLPSP
                     LDIPPAQGVDYHDEPIEREASEDAPFSALAFKIVADPFGKLTYFRVYSGQINKGDEVY
                     NSSKEKRERLGRILLMHANQREDLDVALAGDIVAGLGFKETTTGDTLCDRAHPIILEK
                     MEFPTPVISVAIEPKTKADQDKLGKALKSLSDEDPSFRVHTDEETNQTVIAGMGELHL
                     EILVDRMQREFNVDATVGKPQVAYRETITKKVESVEYRHIKQSGGSGQFAVVKINLEP
                     NPEGGYLFVDKITGGRVPREYIQPTNVGIQSALDNGVLAGYPTVNVIATLVDGQYHDV
                     DSSEMAFKIAGQMAFKKAAEMAKPVLLEPIMAVEVVTPDEYMGDVMGDLSSRRGRIEG
                     MEALGNTQAVRAQVPLSEMFGYSTDLRSRTQGRATYTMQFDSYQEVPNAIAADIIKRV
                     RGE"
     gene            complement(1599..2090)
                     /locus_tag="PLV68_13440"
     CDS             complement(1599..2090)
                     /locus_tag="PLV68_13440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone"
                     /protein_id="HRE02300.1"
                     /translation="MSPDDDVTGGLTHGDLTGDLNAALAAASNNPKKPYQLDKGQCTI
                     TMPLPAGLADGLDELTVTVRLIGEIVSFVSPLAHVTEPEIPADAATALLRRQFFAQQT
                     DGAGFALADREDVLVAQYHWIPTGLTVDAFAAIFGRFVAATLRLRDEVAIMSAQGAPF
                     TIL"
     gene            complement(2087..3220)
                     /locus_tag="PLV68_13445"
     CDS             complement(2087..3220)
                     /locus_tag="PLV68_13445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02301.1"
                     /translation="MDAGDLIQRDLMGAVKSVGKGFTAAGKGIASAAKATGKFVEKTA
                     KSIGNAAVGFGKGLHRSDFNDLNATQYLVSAGGKIEAIDRRVEDDGSIVYYKTGLVTI
                     GEGDRPKIEAYDEAIAMPAGWLPSVTHINGMMVKPNGGLGSALKLQQELERAGGEALL
                     GHDVPSVLYTYSAHRGFVTDLAECVWGKLYQDDDATDRQTQIMLDAVKQQHRTTVSAH
                     SRGTIKTDNAVRNAHAQLSAEYLPALIQDPAVIAEAQQAAQIAARMNAELGLSAAMLT
                     PVYIRLFAKEHAAKKATADMDQFIQLIYAGNAVQFPSASVNLNLVVAGSDPVTIGVGK
                     YFGFAKGSKTKMTDVAGGHGFNQNYAATVADLIMADIQANQRP"
BASE COUNT          581 a         1071 c         1123 g          568 t
ORIGIN      
        1 gatgaaggcc ctcatctggc gcgacgaaga gctgggcgcc aagtgggacg aggtcgagat
       61 cccggccgac atgcaagagc aggccgacca gtaccgccac gagctgctcg agaccatcgc
      121 caccagcgac gacgacttca tggagaagta tctcggtggc gaagacttca ccgaggcaga
      181 catcaagcgg gcgatccgca agggcacgct cgcgttcgac ttcgtgccga tcctgtgtgg
      241 ttcgtcgttc aagaacaagg gcgttcagcc gatgctcgac gccgtcatcg acttcttgcc
      301 gagcccgctc gacatcccgc ccgcgcaggg tgtcgactac cacgacgagc cgatcgagcg
      361 cgaagccagc gaagacgcac cgttctccgc gctggcgttc aagatcgtcg ccgacccgtt
      421 cggcaagctg acctacttcc gggtctactc cggccagatc aacaagggcg acgaggtcta
      481 caacagctcc aaagagaagc gtgagcgcct cggccgcatc ctgttgatgc acgccaacca
      541 gcgtgaagac ctcgatgtcg cgctcgctgg cgacatcgtt gccggcctcg gcttcaagga
      601 gaccaccacc ggcgacacgc tgtgcgatcg ggcccacccc atcatcctcg agaagatgga
      661 gttccccacg ccggtcatca gcgtcgcgat cgagccgaag accaaggccg accaggacaa
      721 gctgggcaag gccctcaaga gcttgtccga cgaggatccc agcttccgtg ttcacaccga
      781 cgaagagacc aaccaaacgg tcatcgccgg aatgggtgag ctccacctgg agatcctggt
      841 cgaccgcatg cagcgcgagt tcaacgtcga cgcgacggtg ggcaagccgc aggtggccta
      901 ccgcgagacg atcaccaaga aggtcgagtc ggtcgagtac cgccacatca agcagtcggg
      961 tggttcgggt cagttcgccg tcgtcaagat caacttggag ccgaaccccg agggcggcta
     1021 cctgttcgtc gacaagatca ccggcggacg cgtgccacgc gagtacatcc agccgaccaa
     1081 cgtgggcatc cagagcgcgc tcgacaacgg cgtgctcgcc gggtacccca cggtcaacgt
     1141 catcgccacg ctggtcgatg gtcagtacca cgacgtcgac tccagcgaga tggccttcaa
     1201 gatcgccggt cagatggcct tcaagaaggc tgccgagatg gccaagccag tgctgctcga
     1261 gccgatcatg gccgtcgagg tcgtcacgcc cgacgagtac atgggcgacg tgatgggtga
     1321 cctcagcagc cgccgcggcc gcatcgaggg catggaggcg ctgggcaaca cccaggccgt
     1381 tcgggcccag gtgccgttgt cggagatgtt cggctactcc accgacctgc gttcgcgcac
     1441 ccagggccgg gccacctaca cgatgcagtt cgactcgtac caagaggtgc cgaacgcgat
     1501 cgcggccgac atcatcaagc gagtccgcgg cgagtaaccg ctgctggtcg tcgccagtcg
     1561 tgaagagccc cgcccgcaag gcggggctct tcacgttctt acaggatcgt gaagggggcg
     1621 ccctgggcgg acatgatcgc cacctcgtcg cggaggcgga gggtggcggc gacgaagcgg
     1681 ccgaagatcg cggcgaacgc gtcgacggtg agcccggtcg gaatccagtg gtactgagcg
     1741 acgagcacat cctcgcggtc ggccagcgcg aagcctgcac catcggtctg ttgcgcgaag
     1801 aactggcggc gcagcagcgc ggtggcggcg tcggcgggga tctcgggctc ggtcacgtgc
     1861 gccaggggcg agacgaacga cacgatctcg ccgatgagcc gcacggtcac cgtcaactcg
     1921 tcgagcccgt cggccagccc ggcgggcagc ggcatggtga tcgtgcactg gcccttgtcg
     1981 agctggtacg gcttcttggg gttgttgctg gccgcggcga gcgcggcgtt caagtcgccg
     2041 gtcaggtcgc catgggtgag gccgccggtc acgtcgtcgt cggggctcat gggcgctgat
     2101 tggcctggat gtcggccatg atcagatcgg caacagtggc ggcgtagttc tggttgaacc
     2161 cgtggccacc ggcaacgtcg gtcatcttgg tcttcgaccc cttggcgaag ccgaagtact
     2221 tgccgacacc gatcgtgacc ggatcggaac cggccacgac caggttgagg ttgaccgagg
     2281 ccgacgggaa ctgcaccgcg ttgccggcgt agatcagctg gatgaactgg tccatgtcgg
     2341 ccgtggcctt cttggctgca tgttccttgg cgaacagacg gatgtagacc ggcgtcaaca
     2401 ttgccgctga caagccgagc tcggcgttca tgcgggccgc gatctgggcc gcttgctgcg
     2461 cttcggcgat gaccgccggg tcttggatca gcgcgggcag gtactcggcg ctgagctgag
     2521 cgtgggcgtt gcggaccgcg ttgtcggtct tgatcgtgcc gcggctgtgg gccgacaccg
     2581 tggtgcggtg ctgttgcttg acggcgtcga gcatgatctg ggtctggcga tcggtggcgt
     2641 cgtcgtcttg gtagagcttg ccccacacgc actcggccag gtcggtgacg aacccgcggt
     2701 gggcgctata ggtgtacagc acgctcggca cgtcgtggcc gagcagcgcc tcgcctccgg
     2761 cccgttcgag ctcttgctgc aacttgagtg cgctgcccag accgccgttg ggcttgacca
     2821 tcatcccgtt gatgtgggta actgacggca accaaccggc cggcatcgcg atcgcctcgt
     2881 cgtacgcctc gatcttgggt cggtcgcctt cgccgatggt caccaagccc gtcttgtagt
     2941 acacgatcga gccatcgtct tcgacccggc gatcgatcgc ttcgatcttg ccgccagccg
     3001 acacgaggta ctgggtcgca ttcagatcgt tgaagtcgga gcgatggaga cccttgccga
     3061 agccgactgc cgcgttcccg atcgacttgg cggtcttctc gacgaacttg cccgtcgcct
     3121 tggcggccga ggcgatgcct ttgccggcgg ccgtgaagcc cttgccgacc gacttcaccg
     3181 cgcccatgag gtcgcgctgg atgaggtcgc cggcgtccat cggttggatg cgagctctca
     3241 gagggatggc tgccgtggcg ccggtgcgtg cgatcgctgc cccggaacgc ggcgcggtct
     3301 gggcggtcgc gcgttggaca cggctgcacg acccgggcgt cgc
//
LOCUS       DAORNF010000351         3338 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_21526_length_3338_cov_1.594883, whole genome shotgun sequence.
ACCESSION   DAORNF010000351 DAORNF010000000
VERSION     DAORNF010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3338
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21526_length_3338_cov_1.594883"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>719
                     /locus_tag="PLV68_13450"
     CDS             <1..>719
                     /locus_tag="PLV68_13450"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRE02302.1"
                     /translation="LAAASMSGAFTNTLFTQTVNYAAKEFDVGKFGQGVGGVVVRAGI
                     VFALPIAFLADRAGRRRMMVLVAWLAPIISALGAIAPTFPVLVATQSLGRPMGLALDL
                     LIAVAVTEEMPKNSRAYALSVLAMASGLGAGIAVIGLPLADLGQAGWRLVYLLALIWL
                     VVAYDITRRVPETRRFQRPHVIAPPLDKARFTQIAAVSFFANIFVAPASFFQNRYLDE
                     VRDLSAAGITIFTLGTATPAA"
     assembly_gap    720..764
                     /estimated_length=45
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <765..1157
                     /locus_tag="PLV68_13455"
     CDS             <765..1157
                     /locus_tag="PLV68_13455"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRE02303.1"
                     /translation="RKRLLAVGLPLGAALLVWSFSTPGFGLWFGAFSAGFVGGITYPA
                     FAVYRTEMFPTGNRGRAAGLITASALIGGSFGLIASGRLLDSGWSYGKTMALMAIGEI
                     VAVTIVVFRYPETAHRDLDELNPEPPLS"
     assembly_gap    1263..1275
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1276..1529)
                     /locus_tag="PLV68_13460"
     CDS             complement(<1276..1529)
                     /locus_tag="PLV68_13460"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02304.1"
                     /translation="MAAALLTHYASDRIVVRSAGTAPAGTINPVVIEVMGEVGIDLLA
                     AGATPKQLTDAAVRASDVVITMGCGDECPFYPGKRYVDWRL"
     gene            complement(1588..>1851)
                     /locus_tag="PLV68_13465"
     CDS             complement(1588..>1851)
                     /locus_tag="PLV68_13465"
                     /inference="COORDINATES: protein motif:HMM:NF012455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HRE02305.1"
                     /translation="HGCARAGRGASAPFAVGAWIGGAYWFTSSTGFANPAVTIARTLS
                     DSFAGIKPSSAPMFVLMQIVGMTVAYGLINFFYPHARPETLDG"
     assembly_gap    1852..1894
                     /estimated_length=43
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1895..2290)
                     /locus_tag="PLV68_13470"
     CDS             complement(<1895..2290)
                     /locus_tag="PLV68_13470"
                     /inference="COORDINATES: protein motif:HMM:NF012455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HRE02306.1"
                     /translation="MLLTTQPRLDLGRRVVAEAIGTCLLVVAIVGSGIMASRLSPGDV
                     GLQLVENAAATAGALIGLIVMFGAVSGAHFNPVVTIVERLVGAMSTDEAVAYIAAQIA
                     GGCAGALLANAMFELPVFQWADRGRASGAL"
     gene            complement(2281..>2471)
                     /locus_tag="PLV68_13475"
     CDS             complement(2281..>2471)
                     /locus_tag="PLV68_13475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02307.1"
                     /translation="LDQDVQERVSCCYAVQDKTWVQGPDNAWEFYTVLADAPDLACSA
                     DPDGGCESEPTPTQGSCC"
     assembly_gap    2472..2486
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2487..2713)
                     /locus_tag="PLV68_13480"
     CDS             complement(<2487..2713)
                     /locus_tag="PLV68_13480"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRE02308.1"
                     /translation="MQVALNVADLDASIAFYSKLFQTPPAKVRDGYANFAIADPPLKL
                     VLFAGGGEPGTLNHIGVEVDSPADVAAAIQRA"
     assembly_gap    2757..2773
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2829..>3024
                     /locus_tag="PLV68_13485"
     CDS             2829..>3024
                     /locus_tag="PLV68_13485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02309.1"
                     /translation="MAAIPTAGTDPIACCAPVRSPRFDDSQAETIAGAFAALADPVRL
                     RLLAHVAAAGGDEVCACDLGT"
     assembly_gap    3025..3101
                     /estimated_length=77
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          484 a         1060 c         1049 g          535 t
ORIGIN      
        1 gttggccgca gcctcgatga gcggcgcgtt caccaacacg ctgttcacgc agacggtcaa
       61 ctatgccgcc aaggagttcg acgtcggcaa gttcggccaa ggcgtcggcg gcgtcgtggt
      121 gcgggcgggc atcgtgttcg ccctgccgat cgcgttcctg gccgatcggg cgggacggcg
      181 gcggatgatg gtgctggtcg cctggctggc accgatcatc tccgccctcg gcgcgatcgc
      241 ccccaccttt cccgtcctgg tcgctaccca atcgctcggg cgcccgatgg ggctggcgct
      301 cgacctgctg atcgccgtcg cggtcaccga ggagatgccg aagaacagcc gcgcctacgc
      361 gctgagcgtg ctggcgatgg ccagcggtct tggcgcgggc atcgccgtga tcggcttacc
      421 cctggctgac cttggccaag cgggctggcg gctcgtctac ctgctcgccc tgatctggct
      481 ggtcgtcgcc tacgacatca cccgccgcgt gcccgagaca cgacggttcc agcgccccca
      541 cgtcatcgcc ccgccgctcg acaaggcccg cttcacccag atcgccgccg tgtcgttctt
      601 cgccaacatc ttcgtggcac cggcatcgtt cttccagaac cgttacctcg acgaggtgcg
      661 cgacctgtcg gcggccggga tcaccatctt caccctcggc accgcgacgc ccgccgcgcn
      721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncgcaag cgcctgttgg
      781 cggtcggtct gccgctcggc gcggcgctgc tggtgtggtc gttcagcaca ccggggttcg
      841 gtctgtggtt cggtgcgttc agcgcggggt tcgtcggcgg catcacctac cccgcgttcg
      901 ccgtctaccg caccgagatg ttccccaccg gcaatcgcgg ccgggccgcc ggcctgatca
      961 ccgcatcggc cctgatcggc ggctcgttcg gcctgatcgc cagcggccgc ctgctcgaca
     1021 gcggctggtc gtacggcaag acgatggcgc tgatggccat cggcgagatc gtcgccgtca
     1081 ccatcgtcgt gttccgctac cccgaaacag cccaccgcga cctcgacgaa ctcaaccccg
     1141 aacccccgct gtcatgacct ggccagctgg cgtctgcaag caggttggca acgagctcgc
     1201 ggacgaggtg atcgatctgg tcacggatga cacggacagc ggccgcgcct tgaccggcag
     1261 ggnnnnnnnn nnnnnagacg ccagtcgacg taccgtttgc cgggatagaa ggggcactcg
     1321 tcaccgcagc ccatcgtgat caccacgtcc gaagcccgca ccgcggcgtc ggtgagttgt
     1381 ttcggcgtgg ccccggcggc gaggaggtcg atgccaacct cgcccatgac ctcgatgact
     1441 accgggttga tcgtgccagc cggtgccgtc ccggcagagc ggacgacaat gcggtcgctg
     1501 gcgtagtggg tgagaagtgc ggcggccatc tgtgatcgtc ctgcgttgtg aacgcagacg
     1561 aagagcactt cgggtcggtc ggcggtgtca gccatcgagg gtctccgggc gagcgtgcgg
     1621 gtagaagaaa ttgatcaagc cgtacgcgac ggtcatgccg acgatttgca tgaggacgaa
     1681 catcggtgcc gacgacggct tgatcccggc gaagctgtcc gacaacgtgc gggcgatggt
     1741 gacggctggg ttggcaaagc ccgtcgagga cgtgaaccag taggcgcctc cgatccacgc
     1801 gcccaccgcg aacggggccg aagcgccgcg ccctgcacgg gcacagccgt gnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncagcgc gcctgaagcc cgtcccctgt
     1921 ccgcccattg gaaaacgggc aactcgaaca tggcgttggc gagcaaggca ccagcgcaac
     1981 cgccagcgat ctgcgccgcg atgtaggcga cggcctcgtc ggtcgacatg gcaccgacga
     2041 ggcgctcgac gatggtgacg accgggttga agtgggcgcc ggagacagcg ccgaacatca
     2101 cgatcaaccc gatcagcgca cccgcggtcg cggctgcgtt ctccacgagt tggaggccga
     2161 cgtcgcctgg cgacagacgg ctggccatga tgccagagcc gacgatggcc acgaccaaca
     2221 ggcacgtgcc gatcgcttca gcgacgacgc ggcgacccag gtccaggcgt ggttgggtgg
     2281 tcagcagcac gatccttggg tgggtgttgg ttcggactcg catccgccgt cggggtcggc
     2341 cgaacaggcg aggtccggcg cgtcggcgag caccgtgtag aactcccagg cgttgtccgg
     2401 cccctgcacc caggtcttgt cttgcaccgc gtagcagcac gacacgcgct cctgaacgtc
     2461 ctggtccagc cnnnnnnnnn nnnnnngccc gctgaatcgc tgccgcgacg tcggctggag
     2521 agtcgacctc gaccccgatg tggttcaacg tccctggctc gccacccccg gcgaacagca
     2581 ccagcttgag tggcggatcg gcgatcgcga agttcgcata gccgtcgcgc accttggcgg
     2641 gcggtgtctg gaagagcttc gagtagaacg cgatcgacgc gtcgagatcg gcaacgttca
     2701 acgcaacctg cacacgggac atgagaactc actccttcgt ttgacgatgg cgactannnn
     2761 nnnnnnnnnn nnnatagttc gacggtcgtc aatgtttttg atcgacgtcg ttcgatagag
     2821 ttcgcgccgt ggccgccatc ccgaccgccg gcaccgaccc gatcgcttgc tgtgcacccg
     2881 tgcgctcccc ccggtttgac gactcgcagg ccgaaacgat cgccggcgcc ttcgccgcgc
     2941 ttgctgaccc ggttcggctg cgcctgctcg cgcacgtggc tgctgcaggc ggtgacgagg
     3001 tttgcgcctg cgatctcggc acggnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn naaagcgtgg gctgtgggtc
     3121 tggtatcgga tcgtccccac ccgcctcgac gccctccgcg ccgtcctcgc ctgacccaca
     3181 tccttccgac tgttcggggt tgcactaccg cgtcagcgct tcggggacga tcttggcagg
     3241 gttggggcta tgggctgacg gggagggtgg agagccaggt ggtgatggct tcggcgatgg
     3301 ggatgtcgca ggctttgaga tcgtggccct taccgggc
//
LOCUS       DAORNF010000352         3338 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_21528_length_3338_cov_1.314956, whole genome shotgun sequence.
ACCESSION   DAORNF010000352 DAORNF010000000
VERSION     DAORNF010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3338
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21528_length_3338_cov_1.314956"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>228)
                     /locus_tag="PLV68_13490"
     CDS             complement(<1..>228)
                     /locus_tag="PLV68_13490"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02310.1"
                     /translation="PSRTALATGMFGTRNGVVSHGGVAADPYPEGPNRAFMGSLMRAS
                     WAWRLYQAGYHTASISSFPFRHSASWWNHGYM"
     assembly_gap    229..287
                     /estimated_length=59
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(402..968)
                     /locus_tag="PLV68_13495"
     CDS             complement(402..968)
                     /locus_tag="PLV68_13495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02311.1"
                     /translation="MASPPQRHTDLLTAVIMEDPPLFRPDPGQAPSLEGNALLDGFRL
                     MRTSMPQLQASGMSLEMLTTVLSNAPDTTRTGTFGQMLHRDGIETMAAGLLRVDASVL
                     DPVLDGSLVFTVDPDQPFGVPALLVPADPTKPDAVASPKATAWFAANSPGTEVVVATG
                     AGHLIHDEVHSRDTFRDTVLSFLSRIAP"
     gene            complement(1314..>1570)
                     /locus_tag="PLV68_13500"
     CDS             complement(1314..>1570)
                     /locus_tag="PLV68_13500"
                     /inference="COORDINATES: protein motif:HMM:NF020496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HRE02312.1"
                     /translation="GPSPFHFVAVPDDECLDIRAIAPDISYGWGVIPATVTIGATTFS
                     TSLFPKNGGYLVPIKDAVRRAEGVDIGDDVAIRLAVPLHR"
     assembly_gap    1571..1794
                     /estimated_length=224
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1795..>2010
                     /locus_tag="PLV68_13505"
     CDS             <1795..>2010
                     /locus_tag="PLV68_13505"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRE02313.1"
                     /translation="PAEIERLAEWFKLLGDPTRMRILYALLEVGELCVGDLAASVDMP
                     ESTVSHALRWLKSSRVVRARRAGKMAF"
     assembly_gap    2011..2135
                     /estimated_length=125
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2136..2577
                     /locus_tag="PLV68_13510"
     CDS             <2136..2577
                     /locus_tag="PLV68_13510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02314.1"
                     /translation="GGYFPLTLRYSLDVPVLLPDAPDGRDRCVHRLTETLRDAPGISQ
                     VHLLVAEPGVPARLCMHFDPEVTSLAHIRDLAEGTGATLTEQVGHRVWRVAGITHQRR
                     ARTVAESLRRVGGVLEADVAPGLVRVEVERFLLGHDRRPSQIFA"
     assembly_gap    2622..2722
                     /estimated_length=101
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2723..>3338
                     /locus_tag="PLV68_13515"
     CDS             <2723..>3338
                     /locus_tag="PLV68_13515"
                     /inference="COORDINATES: protein motif:HMM:NF012350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02315.1"
                     /translation="EGDGDGGHRHGRFGERAERGAAAASGALWLIGLLMELTGVGSSG
                     MHTAVFVVAGICGGAFTLAEAVASIRNRRFEIDFLMLVAAAGAWTLGKWAEGALLLAL
                     FSMGHALEGFALGRARRSIAALGALAPEVATVRDGAVERVVAVGDLRAGDVVVVKPHE
                     RIAADGFVVAGHSSVDQAVLTGESIPVDKQPVADAVLAAAAPDSV"
BASE COUNT          405 a          895 c         1073 g          456 t
ORIGIN      
        1 catgtagccg tggttccacc aggaggcgga gtggcggaac ggaaagctgc tgatcgatgc
       61 ggtgtggtac ccggcctgat acagccgcca cgcccacgag gcgcgcatca gcgaacccat
      121 gaatgcccgg ttgggacctt ctgggtaggg gtcggccgcg acgccgccgt gggagacaac
      181 gccgttgcgg gtgccgaaca tgcccgtcgc caatgcggtg cggctgggnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnggg cgagcgcgtc
      301 gatgttgggc gtcgtgttgc ggtggtagcc ggagcagccc aggtggtcgt ggcgcagggt
      361 gtcgatgtcg aagtagatga tccgcacggc gccgcggtgc gtcagggagc gatgcgtgac
      421 aagaacgaca acacggtgtc gcggaaggtg tcgcgcgagt gcacctcgtc gtggatcagg
      481 tgcccggcgc cggtggcgac gaccacctcg gtgccgggcg agttggccgc gaaccacgcc
      541 gtcgccttcg ggctcgcgac cgcgtcgggc ttggtcgggt cggcaggaac cagcagtgcg
      601 ggcacgccga atggttggtc ggggtcgacg gtgaagacca acgatccgtc gagcaccggg
      661 tcgagcacgg acgcgtcgac ccgcaagagc ccggcggcca tcgtctcgat gccgtcccgg
      721 tgcagcatct gcccgaaggt gcccgtgcgc gtcgtgtcgg gcgcgttgga cagcaccgtg
      781 gtgagcatct ccaacgacat gccgctcgcc tgcaactgcg gcatcgaggt gcgcatcaga
      841 cggaagccgt cgagcagggc gttgccttcc agcgagggtg cctggcccgg atcgggtcgg
      901 aacagaggcg ggtcctccat gatcaccgcg gtcagcagat cggtgtggcg ctgcggtggt
      961 gatgccaccg agggaatggc ccatcaccac gcagggctgc ccggcagcct gttgcagcgc
     1021 ggcgaccgcg tcgctcacgt agccgttctc gtcgtatgcg cctggcgcgc ggtcggattc
     1081 gccgtggccg cggaagtcca gtcgcagcac gcggaaccgc tcggcaagcg tcggcaccaa
     1141 ccattcccac gtgcgtgccg acgaggtgat gccgtgcagc aacaggatcg ggggagcgga
     1201 cgggtcaccg tcttcgagca ccttgagggt gatgtcgccg ttgcgtacct gcatgcgcgg
     1261 cgacgctact gccgggcgta cgcagcccgc cggttgggcg cgacggctgg cggtcagcgg
     1321 tggagtggga cggcgaggcg gatcgccacg tcgtcaccga tgtcgacgcc ctcggcgcgg
     1381 cgcacggcgt ctttgatcgg caccaggtat ccaccgttct tggggaacaa cgaggtcgag
     1441 aacgtggtcg cgccgatcgt caccgtcgcc gggatcacgc cccagccgta actgatgtcg
     1501 ggcgcgatcg cccggatgtc gaggcactcg tcgtcgggta ccgccacgaa gtggaacggt
     1561 gacgggcctc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnccccgg
     1801 ccgagatcga gcgtctggcc gagtggttca agctgttggg ggatccgacg cggatgcgca
     1861 tcctgtacgc cctgctcgag gtgggtgagc tgtgcgtcgg cgatctggcg gccagcgtcg
     1921 acatgcccga atcgaccgtg tcgcacgcac tgcggtggct gaagtcgtcg cgtgtggtgc
     1981 gcgcccggcg ggcggggaag atggccttct nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncggcg gctacttccc gctgacgctg
     2161 cgctactcgc tcgacgtgcc ggtgctgttg cccgacgccc ccgacggccg cgaccggtgc
     2221 gtacaccgcc tgaccgaaac cctgcgcgat gcacccggta tctcccaggt ccacctgttg
     2281 gttgccgagc cgggggtacc cgcccggttg tgcatgcact tcgacccgga ggtcacctcg
     2341 ctggcccaca tccgcgatct cgccgagggc accggtgcca cgctgaccga acaggtcggc
     2401 caccgggtct ggcgtgtcgc gggcatcacc catcagcgcc gggcgcgcac ggtggcagag
     2461 tcgttgcgcc gcgtcggcgg tgtactcgag gccgacgtcg ccccgggtct ggtgcgggtc
     2521 gaggttgagc gcttccttct cggccacgat cgacgaccaa gccagatatt tgcgtagggc
     2581 ctcatcgagg tcttgcagcc gaaccttgtc ggcggcgagg annnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nngcgagggc gacggcgacg gcggccatcg gcatggccgc
     2761 ttcggcgagc gggccgaacg tggtgctgcc gccgcgtcgg gggcactgtg gttgatcggc
     2821 ctcctgatgg agttgaccgg tgtgggcagc agcgggatgc acacggcggt gttcgtcgtg
     2881 gcgggcatct gcggtggcgc gttcacgttg gccgaagccg ttgccagcat ccgcaaccgg
     2941 cggttcgaga tcgacttcct gatgctggtc gcagcggccg gggcatggac gctgggcaag
     3001 tgggcagagg gggcgctgtt gctcgcgctg ttcagcatgg gccatgcatt ggaagggttt
     3061 gccttggggc gggcgcggcg gtcgatcgcg gccctcgggg cactggcgcc cgaggtcgcc
     3121 acggtgcgcg acggtgcagt cgagcgggtc gtcgcggtcg gcgacctgag ggccggcgac
     3181 gtcgtcgtcg tcaaaccgca cgagcggatc gcggccgacg gcttcgtcgt cgccggtcac
     3241 agcagcgtcg atcaggcggt gctcacgggc gagagcattc cggtcgacaa gcagccagtg
     3301 gccgatgcgg tgctcgctgc agcggcgccg gactcggt
//
LOCUS       DAORNF010000353         3325 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_21647_length_3325_cov_1.912232, whole genome shotgun sequence.
ACCESSION   DAORNF010000353 DAORNF010000000
VERSION     DAORNF010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3325
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21647_length_3325_cov_1.912232"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="PLV68_13520"
     CDS             complement(<1..272)
                     /locus_tag="PLV68_13520"
                     /inference="COORDINATES: protein motif:HMM:NF013781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HRE02316.1"
                     /translation="MSIISDIFRYTSTEMPKFNSISISGYHMQEAGATADLELGYTLA
                     DGVEYVRAGLASGLEIDAFAPRLSFFWAIGMNFFMEVAKLRAARLL"
     assembly_gap    290..460
                     /estimated_length=171
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<461..917)
                     /locus_tag="PLV68_13525"
     CDS             complement(<461..917)
                     /locus_tag="PLV68_13525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HRE02317.1"
                     /translation="MSLIPDFSTIPLVDPAATPSTDRTAWSRAGGAAVAEGWATPEGI
                     DVAALYDASDVADLDFLGTHPGLVPFLRGPYPTMYVNQPWTVRQYAGFSTADESNAFY
                     RRNLAAGQKGLSIAFDLATHRGYDSDHPRVAGDVGMAGVAIDSILDMQQL"
     gene            complement(928..>1446)
                     /locus_tag="PLV68_13530"
     CDS             complement(928..>1446)
                     /locus_tag="PLV68_13530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02318.1"
                     /translation="PVPPPPVPTSTATGVTQHGLVKHRLAEPFEALRDRVDAMVRAGA
                     QRPSVFLANLGTAATFTARTTFAKNLFEVAGAATVAGAGTTDPTELVAQFAASGASVA
                     CLCSSDAVYADIGADCAAALTAHGLAGLYLAGRPKDAVEQLAAAGVDVFVTAGADIEA
                     ILSDLLARLNVA"
     assembly_gap    1447..1456
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1457..2894)
                     /locus_tag="PLV68_13535"
     CDS             complement(<1457..2894)
                     /locus_tag="PLV68_13535"
                     /inference="COORDINATES: protein motif:HMM:NF013781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase subunit beta"
                     /protein_id="HRE02319.1"
                     /translation="MTDSGPPSAAGPTATAQIDLAGEFEPSTWEQWLHTAAKALKRDL
                     GPDDDPAAVVAKLRSTTYDGIVIEPLYTAEHHAASAAPGLPGTAPLVRGRTAAATREH
                     GWDVRQRVIAGSDGPSALDELECGATSVWLDVTRLDPADPVAFTAALSSALDGVLFDL
                     AGVVLDAGERWAEAARPLIQLWRSSPDLAAVTGNLGADPIGELAERGGNDASDAFAAA
                     IELVRVVQTDLAGVRVFAVDATRYHDAGATDADELALGIAAGLATVRAMADAGIDLDT
                     AFAQIEFRLAATADQFATIAKFRAARRLWSRIAEVCGVAEAAASTPLHAVTSRAMMSR
                     HDPWVNLLRGTTACFGAGVGGADAITVLPYDEFLGGEGGSELGRRIARNTQSILAKES
                     NLARVIDPGGGSWFVESFTAQLAEAAWQRFQQIEGAGGIVAAIADGSVAAGLDAAWQA
                     RRKNLVTRRDPLTGVSEFPNVMEAPVDIE"
     gene            complement(3006..>3325)
                     /locus_tag="PLV68_13540"
     CDS             complement(3006..>3325)
                     /locus_tag="PLV68_13540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRE02320.1"
                     /translation="RAAKAMDVMGNQQARVWVSAVKAMVPVRVCQIIDEAIQIHGATG
                     VSQWTPLARMYASQRTLRLADGPDEVHWHVVGRAELNRYEDDHAPAVVNPAAGKDGIF
                     SGP"
BASE COUNT          487 a         1093 c         1102 g          462 t
ORIGIN      
        1 cacagcaggc gggccgcgcg cagcttggcg acctccatga agaagttcat gccgatcgcc
       61 cagaagaacg acagacgcgg cgcgaacgcg tcgatctcca gaccggaggc gagcccggcg
      121 cgcacgtact cgacgccgtc ggccagggtg tagccgagct ccaagtcggc cgtcgccccc
      181 gcctcttgca tgtggtagcc gctgatcgag atcgagttga acttcggcat ctccgtcgac
      241 gtgtagcgga agatgtcgga gatgatgctc atcgacgggg cgggcggatn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn acagttgctg catgtcgagg
      481 atcgagtcga tcgccacgcc ggccataccg acgtcaccgg cgacacgggg gtggtcgctg
      541 tcgtagccgc ggtgggttgc gaggtcgaac gcgatcgaca gacccttctg acccgcggcc
      601 aggttgcggc gatagaacgc gttgctctcg tccgcggtgg agaatccggc gtactgacga
      661 acggtccacg gctggttgac gtacatcgtg gggtacgggc cgcgcaggaa cggcaccagg
      721 ccggggtggg taccgaggaa gtcgaggtcg gcaacatcgc tcgcgtcgta cagcgcggcg
      781 acgtcgatgc cttcgggggt cgcccaccct tcggccacgg cggcgccacc tgcacgtgac
      841 catgcggtgc ggtcggtgct cggtgtcgcc gcggggtcga ccagggggat cgtggaaaag
      901 tcggggatca agctcatggt cggctcctca tgccacgttc aggcgggcca gcagatcgga
      961 caggatcgct tcgatgtcgg ccccggcagt gacgaacacg tcgacacccg cggcggccaa
     1021 ctgttccacc gcatccttcg gccgtccggc cagatacagc ccggcgaggc cgtgggccgt
     1081 cagcgccgcg gcgcagtccg cgccgatgtc ggcgtatacc gcgtcggacg aacagagaca
     1141 ggccacgctc gcgccgctgg cggcaaactg ggcgaccaac tccgttgggt cggtggtgcc
     1201 agctccggcg acggtagccg cacctgcgac ttcgaacagg ttcttggcga acgtggtgcg
     1261 ggcggtaaag gtggccgccg tgcccaggtt ggcgaggaac accgacggac gctgcgcacc
     1321 cgcgcggacc atcgcgtcga cccggtcgcg cagcgcttcg aacggctcgg ccaggcggtg
     1381 cttgaccagg ccgtgctgag tcacccccgt tgctgttgac gtcgggactg gcggtggcgg
     1441 cactggnnnn nnnnnngctc gatgtcgacc ggagcttcca tgacgttggg gaactcgctg
     1501 acgccggtga gcggatcgcg acgggtgacc aggttcttgc gccgggcctg ccaggccgcg
     1561 tcgagaccgg cggccacgct gccgtcggcg atcgcggcga cgatgccgcc cgcgccttcg
     1621 atctgctgga accgctgcca cgcggcctcg gccagttgcg ccgtgaacga ctcgacgaac
     1681 cacgacccgc cacccgggtc gatcacccgc gccaggttcg actccttggc caggatcgac
     1741 tgggtgttgc gggcgatgcg ccgaccgagc tcagaaccgc cctcaccgcc gaggaactcg
     1801 tcgtacggca gcaccgtgat cgcgtcggcc ccgccgacac cggcgccgaa gcaagccgtc
     1861 gtgccgcgca gcagattgac ccacgggtcg tggcggctca tcatcgcccg cgaggtcacg
     1921 gcatgcagcg gcgtgcttgc ggccgcttca gcgacaccac acacctccgc gatgcgcgac
     1981 cacaaccggc gggccgcgcg gaacttggcg atggtcgcga actggtcggc cgtcgccgcg
     2041 aggcgaaact cgatctgggc gaacgccgtg tcgaggtcga tgcctgcgtc cgccatcgcc
     2101 cgcaccgtgg cgagaccagc agcaatcccc agagccaact cgtcggcatc ggtggcgccc
     2161 gcgtcgtggt agcgggtcgc gtcgacggcg aacacccgca caccggccag gtcggtctgc
     2221 acgacccgga cgagctcgat cgctgccgcg aacgcatcgc tcgcgtcgtt gccaccgcgc
     2281 tcggccaact cgccgatcgg gtcggcaccg aggttgccgg tcaccgcggc gaggtccggg
     2341 ctcgaccgcc acagttggat caacggtcga gcggcctcgg cccaccgctc gcctgcgtcg
     2401 agcacgaccc ctgccaggtc gaacagcacg ccgtcgagcg cggagctgag cgccgcggtg
     2461 aacgcgaccg gatcggccgg atcgaggcgc gtcacgtcga gccacaccga ggtcgcgccg
     2521 cactccaact cgtccaacgc ggacggcccg tcggagccag cgatgacccg ctggcgtacg
     2581 tcccaaccgt gctcacgggt ggccgcagca gtgcggccgc gcaccaacgg tgccgtaccg
     2641 ggcagacccg gggctgcgct ggcggcatgg tgctcggccg tgtacaacgg ctcgatcacg
     2701 atgccgtcgt aggtggtgct gcgcagcttg gccacgaccg cggcgggatc gtcgtcgggt
     2761 ccgaggtcgc gcttcaatgc cttggccgcg gtgtgcagcc actgctccca ggtagacggt
     2821 tcgaactcgc cggcgaggtc gatctgcgcg gtggcagtcg gcccggcggc actggggggg
     2881 ccggagtcgg tcatcgtagg gcgagtgtaa tgggcacgct gtcgcgcaac ccagtgccgc
     2941 cccggcaccc catgccggag caagcctgga tcaggcctgc agccgcgcac cgatcaccgt
     3001 gacgatcacg gtccggagaa gatgccgtcc ttgcccgccg cggggttgac caccgcgggc
     3061 gcgtggtcgt cttcgtagcg gttcagctcg gcccggccga ccacgtgcca gtgcacctcg
     3121 tcggggccgt cggccaggcg caacgtacgc tgggaggcgt acatccgtgc cagcggcgtc
     3181 cattgcgaca cgccggtcgc tccgtggatc tggatcgctt cgtcgatgat ctgacacaca
     3241 cgcaccggca ccatcgcctt gaccgcgctg acccacaccc gtgcttgctg gttgcccatc
     3301 acgtccatcg ccttggccgc gcgca
//
LOCUS       DAORNF010000354         3310 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_21790_length_3310_cov_1.329954, whole genome shotgun sequence.
ACCESSION   DAORNF010000354 DAORNF010000000
VERSION     DAORNF010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3310
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21790_length_3310_cov_1.329954"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>282
                     /locus_tag="PLV68_13545"
     CDS             <1..>282
                     /locus_tag="PLV68_13545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02321.1"
                     /translation="GTRMAWFRDAVAQVRAVNDRLAVKLVPLAIGAMGVLQLGIPVVI
                     AAVAYRVSGGQVSVAIGVSFLVLILRVYGPLLAVAVQVESLRLADASLE"
     assembly_gap    283..424
                     /estimated_length=142
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <425..1104
                     /locus_tag="PLV68_13550"
     CDS             <425..1104
                     /locus_tag="PLV68_13550"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRE02322.1"
                     /translation="TMTAIVGPSGAGKSTLVHLIARFWDASRGQVCIGGADVTTLTAA
                     QLFDMVTVVFQDVYLFQGTIRDNIAFGRPDATDAEVRSAAAAAQVDAFVSSLPDGYDT
                     AVGEGGATLSGGERQRISIARAILKGSPIVLLDEATASIDPLNEREVRLALAELVRGR
                     TVIVVAHRLTTIRSADQILVLDDHRIVDSGTHAELMTRPGVYVELWNQRQRAVAWRID
                     RQRTPSD"
     gene            complement(1096..1842)
                     /locus_tag="PLV68_13555"
     CDS             complement(1096..1842)
                     /locus_tag="PLV68_13555"
                     /inference="COORDINATES: protein motif:HMM:NF019599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcnI family protein"
                     /protein_id="HRE02323.1"
                     /translation="MSARSTRRVVTALALACGASVAIGAHGALAHVGTDKSEITAGAS
                     TTLTFAIGHGCDDLPTREMKFQIPDSVLNAAPQMKPGWTITTEREDLAEPVESAHGPA
                     QTDRVSVITFTATDGFEVDPHYRDTFTLAFRAPAETGTLFFKTIQDCGGGNNYAWIEE
                     WDGAGAEPDSPAPSVEVVAATDGDDDGHGAAPTTAATTAPSVTVGGSSESGSDSDSGN
                     GLAIAALIVAIAGVGVGGAALARSRRPQSV"
     assembly_gap    2041..2153
                     /estimated_length=113
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2154..>2521)
                     /locus_tag="PLV68_13560"
     CDS             complement(<2154..>2521)
                     /locus_tag="PLV68_13560"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRE02324.1"
                     /translation="SQAGDKLKVGSIGKPIWGIEAKVVGEHGDELGDGEENVGEILLR
                     GHNIMKGYYKNPTATAAAIQNGWFHTGDLGYRDSDGFYFIVDRKKDLVIRGGFNVYPR
                     EIEEVLYTHPAIAEAAVIGKP"
     assembly_gap    2522..2674
                     /estimated_length=153
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2675..>3310)
                     /locus_tag="PLV68_13565"
     CDS             complement(<2675..>3310)
                     /locus_tag="PLV68_13565"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRE02325.1"
                     /translation="QLPNVPHFLFAYFGILKAGMVMVPLNPLLMAPEVAYHLQDSDTK
                     LLIAFELFAAEAMKGAAEVPGVRVAVVSVFGTPTPEGAMSFDELLTGTDTGDIVALGS
                     DDTAVILYTSGTTGKPKGAELTHFQLYMNCTVAGELFDYRPDDIVLAVLPLFHVFGLS
                     SVLNIAVRFGGTMTLVPRFEVEPVLDAMESTRCTLFAGVPTMYVALLAADTS"
BASE COUNT          426 a          946 c         1029 g          501 t
ORIGIN      
        1 ccggcactcg catggcgtgg ttccgcgacg cggtggccca ggtgcgggcg gtcaacgacc
       61 gcctggcggt caagctggtg ccgttggcga tcggggcgat gggcgtgctg caactcggca
      121 ttcccgtggt gatcgccgcg gtggcctacc gagtctcggg tggacaggtc agcgtcgcga
      181 tcggcgtgtc gttcctggtg ctgatcctgc gcgtgtacgg accgttgctc gccgtggcgg
      241 tgcaggtcga gagcctgcgt ctggccgacg cctcgctgga gcnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnngcacga tgaccgcgat cgtcggcccg tcgggcgccg gcaagtccac cctggtgcac
      481 ctgatcgccc gcttctggga cgcgtcgcgt ggccaggtgt gcatcggcgg tgccgacgtc
      541 accacgctga ccgcagccca gctgttcgac atggtgaccg tggtgttcca ggacgtctac
      601 ctgtttcagg gcacgatccg cgacaacatc gctttcggtc gccccgacgc caccgatgcc
      661 gaggtgcgct ccgccgcggc cgcggcccag gtcgacgcgt tcgtctcgtc actgcccgac
      721 ggctacgaca cggcggtggg ggagggcggt gccaccctgt cgggtggcga gcgccagcgg
      781 atctcgatcg cccgggcgat cctgaagggc tccccgatcg tgctgctcga cgaggcgacc
      841 gcctcgatcg acccgctcaa cgagcgcgag gtgcggttgg cgctggccga gttggtccgc
      901 ggccgcacgg tgatcgtcgt tgcccaccgc ctgaccacga tccgctcggc cgaccagatc
      961 ctggtgctcg acgatcatcg catcgtcgac agcggtaccc acgccgagtt gatgacgagg
     1021 ccgggggtat acgtcgagtt gtggaaccag cgccaacgcg ccgtcgcctg gcgcatcgac
     1081 cgccaacgca ctccctcaga ctgactgggg gcgacggctg cgggcgaggg ccgcaccgcc
     1141 gacgccgaca ccggcgatgg ccacgatcag cgccgcgatc gcgagtccgt tgccgctgtc
     1201 cgagtccgac cccgactcgc tcgagccgcc gacggtcacg ctcggtgccg tcgtcgccgc
     1261 agtcgtcgga gccgcgccgt gaccatcgtc gtcaccatcg gtggcagcga cgacctcgac
     1321 gctcggagcg gggctgtcgg gctcggcgcc agcgccgtcc cactcctcga tccacgcgta
     1381 gttgttgccg ccaccgcagt cctggatcgt cttgaagaac agcgtgccgg tctcggccgg
     1441 ggcgcggaac gccagggtga aggtgtcgcg gtagtgcggg tcgacctcga agccgtcggt
     1501 ggcggtgaag gtgatgaccg agacccgatc ggtctgggcc ggtccgtggg cggactcgac
     1561 cggctcggcg agatcctctc gctcggtggt gatcgtccag cccggcttca tctggggagc
     1621 ggcgttgagg acggagtccg ggatctggaa cttcatctcc cgcgtcggca ggtcgtcgca
     1681 gccgtgaccg atcgcgaagg tcagcgtcgt cgaggccccc gcggtgatct cggacttgtc
     1741 ggtgccgacg tgggccagcg caccatgagc accgatggcc acactggcgc cgcacgcaag
     1801 ggcgagcgcg gtgacgacgc gacgggtgga acgagcagac atttgggggc ctccaggcga
     1861 gaacggacat cggtccggaa cgtgagtggt gctcgtggca ccgttcgatc agtcgtcgcg
     1921 agatcggggt tggttcccgt cgcggcggtt tcgtatcagt cgcggagatc tttcttcaag
     1981 atcttgccgg tcgggccctt ggggagcgcg tcgatgatca tgatgtgccg cggatacttg
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnggcttgc
     2161 cgatcaccgc ggcttcggcg atcgccgggt gggtgtagag cacctcttcg atctcgcggg
     2221 ggtagacgtt gaatccgccg cggatgacca ggtccttctt gcggtcgacg atgaagtaga
     2281 acccgtcgct gtcgcggtag ccgagatcgc cggtgtggaa ccatccgttt tggatcgcgg
     2341 ccgcggtggc cgttgggttc ttgtagtagc ccttcatgat gttgtggcca cgcagcaaga
     2401 tctcgccgac gttctcctcg ccgtcgccca actcgtcgcc gtgctcgccg accaccttgg
     2461 cctcgatgcc ccagatcggc ttgccgatgg agcccacctt cagcttgtcg cccgcctggc
     2521 tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngacgtg tcggccgcga gcagcgcgac
     2701 gtacatggtc ggcacaccgg cgaacaaggt gcagcgggtg gactccatcg cgtcgagcac
     2761 cggctcgacc tcgaaccggg gcaccaacgt catcgtcccg ccgaagcgaa cggcgatgtt
     2821 gagcacgctg gaaaggccga acacgtggaa cagggggagc acggcgagca cgatgtcgtc
     2881 gggccggtag tcgaacagct caccggcgac cgtgcagttc atatacagct ggaagtgggt
     2941 gagttcggcc cccttgggct tgcccgtggt gccactggtg tagaggatga cggcggtgtc
     3001 gtcggagccc agcgcgacga tgtcgcccgt gtcggtgccg gtgaggagct cgtcgaacga
     3061 catcgcgccc tcgggtgtgg gtgtgccgaa cacgctgacc accgccactc gtacgccggg
     3121 cacctcggcg gcgcccttca tcgcctcggc ggcgaacagt tcgaacgcga tcagcagctt
     3181 cgtgtcgctg tcctgcaggt gataggcgac ctcgggggcc atcagcagcg ggttgagcgg
     3241 caccatcacc atccccgcct tgaggatgcc gaagtaggcg aacaggaagt ggggcacgtt
     3301 cggcaactgg
//
LOCUS       DAORNF010000355         3308 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_21806_length_3308_cov_1.718106, whole genome shotgun sequence.
ACCESSION   DAORNF010000355 DAORNF010000000
VERSION     DAORNF010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3308
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21806_length_3308_cov_1.718106"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>211)
                     /locus_tag="PLV68_13570"
     CDS             complement(<1..>211)
                     /locus_tag="PLV68_13570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02326.1"
                     /translation="DDVDVWSSFTGESPVWQDDDSPDPSAGYESVAPHVRKGQMDVSG
                     QHAAYYDDPDPSSPSGAIRRDAPGRI"
     assembly_gap    212..224
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    594..607
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<608..1051)
                     /gene="frr"
                     /locus_tag="PLV68_13575"
     CDS             complement(<608..1051)
                     /gene="frr"
                     /locus_tag="PLV68_13575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HRE02327.1"
                     /translation="MIEETLLEAIDKMDKAVEHVQSQFTTVRTGRATPALVDRIVVDY
                     YGSPVPLQQLAGFQVPEARQLIIKPHDRSALNVIEKAIRESDLGVAPSNDGVVIRINI
                     PALTEQRRKEYVKIVKSLAEDGRIAVRNLRRDARKHLEAAEKAGAI"
     gene            complement(1183..1905)
                     /gene="pyrH"
                     /locus_tag="PLV68_13580"
     CDS             complement(1183..1905)
                     /gene="pyrH"
                     /locus_tag="PLV68_13580"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HRE02328.1"
                     /translation="MEADRLQTVGEALAGPSGSGLDGPTLAQTAQEILDVRENLGVDV
                     AVVVGGGNFWRGRTGAMTGMDATQSDHIGMLGTVMNALALQDALERAGQATRVQSAIE
                     MPRIAEPYIRRRAMRHLEKGRVVIFAAGTGNPFFTTDTAAALRAAEIDANALLKGTHS
                     GVDGVYSADPRVDAAATKYRRVSYLDVMTKDLKVMDATAIAFCRDNKLPIVVFDMSAE
                     GALRAILEGDDVGTIITDSPTA"
     gene            complement(1925..2713)
                     /gene="tsf"
                     /locus_tag="PLV68_13585"
     CDS             complement(1925..2713)
                     /gene="tsf"
                     /locus_tag="PLV68_13585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HRE02329.1"
                     /translation="MTISAKDISALRQSTGAGMMDCKKALEASDGDVEAAKQWLREKG
                     LSAAAKRDERESAQGVVALLIDGNVGGIVKLKSETDFVASSDQFKAEADALVGLVVHK
                     GTDAVAERAASIEDLKIVLKENISIGEVVRFDAAEGNLLESYLHVQGGRGVNAVLLEM
                     SGATPELAHDVAVHIAFARPKYLSKDDVPADVVAAERATLETISRNEGKPEAALPKII
                     EGRLQGFFKEVALLDQPYAKDDKVSITQLIGSASIIRFAQVEIG"
     gene            complement(2716..>3308)
                     /gene="rpsB"
                     /locus_tag="PLV68_13590"
     CDS             complement(2716..>3308)
                     /gene="rpsB"
                     /locus_tag="PLV68_13590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HRE02330.1"
                     /translation="MKVSGEFDAMPKKEALLLTRELEKLQKNLGGIRGMAQRPDAVFV
                     LDTKKEHIAVTEANKLKIPIIAVVDTNVDPDIIQYPIPGNDDAIRANALMSRVIAEAI
                     EEGRYIASRRNPQTAPPKRSADEEAEYMAAQADARKQAAAAQAERDARLTAAKANPAD
                     ADVPVEADVAAETEVAAEAVVEAVAPVAESTESPES"
BASE COUNT          474 a         1111 c         1083 g          613 t
ORIGIN      
        1 tgatgcgacc gggcgcatcg cgccggatcg cgcccgacgg gctgctggga tcggggtcgt
       61 cgtagtacgc ggcgtgctgg ccgctgacgt ccatctgacc cttccgaacg tgcggagcga
      121 ccgactcgta gccggccgac gggtcgggcg aatcgtcgtc ctgccacacc ggcgactccc
      181 cggtgaagct cgaccacacg tcgacgtcgt cnnnnnnnnn nnnnctcgcc cgtgcccccc
      241 gccgtccacg gcggtagcgg gccggtgtcg tcgccgaagg acagtcgccc gcggtcgtcg
      301 tcgggatcgc cgaacaacgg cggtccgaaa tcgtcgtcac ttcgccgacg gcgtgcacgc
      361 cactggtcgt cgctcatgac agccccccaa gtccattgtt cgccggtgca gcgttgccgc
      421 acacgtcacc cgtgcttcga gcagttcttg cgctgttcat acctcgacca gctcctgcgc
      481 cgttcatact tcgaggagct cctgctcctt gcgcccacac gcccggtcga tcgactcgac
      541 gtggtcgtgg gtgatcttct ccatctcctt ctcggcccgc tccaactcgt cggnnnnnnn
      601 nnnnnnngat cgcaccggcc ttctcggcag cttcgaggtg cttgcgggca tcgcggcgca
      661 ggttgcgcac cgcgatccgg ccgtcctcgg ccagcgactt gacgatcttg acgtactcct
      721 tgcggcgctg ctcggtgagc gccgggatgt tgatccggat cacgacgccg tcgttggagg
      781 gggccacgcc gaggtcgctc tcgcggatcg ccttctcgat cacgttgagc gcgctgcggt
      841 cgtgcggctt gatgatcaac tgccgcgcct cgggcacctg gaacccagcg agctgttgca
      901 gcggtacggg cgagccgtag tagtcgacca cgatccgatc gaccagcgcg ggcgtggccc
      961 ggccggtgcg caccgtggtg aactgggact gcacgtgctc gacggccttg tccatcttgt
     1021 cgatggcttc caacaacgtc tcttcgatca cgggcgcaat cgtaacgacc tggcgcgacg
     1081 caccgcgtgc atcggggtat tcacgccccc gcggcccaca cgcaggcggc ccaaatctca
     1141 ggcagccaag actcaggcag ccaaaactca ggcagccaaa actcaggcag ttggcgaatc
     1201 ggtgatgatc gtgccgacgt cgtcgccttc gaggatcgcc cgcagcgcac cctcggccga
     1261 catgtcgaac acgacgatcg gcagcttgtt gtcacggcag aaggcgatcg ccgtggcgtc
     1321 catcaccttg aggtccttcg tcatcacgtc gaggtagctc acccgccggt acttggtcgc
     1381 cgccgcgtcg acgcgagggt cggccgagta gacgccgtcg acaccgctgt gcgtgccctt
     1441 cagcaaggca ttggcgtcga tctcggccgc acgcagggca gccgccgtgt cggtggtgaa
     1501 gaacgggttg ccggtgcccg ccgcgaagat gacgacccgc cccttttcga ggtgacgcat
     1561 cgcccggcga cggatgtacg gctcggcaat gcgcggcatc tcgatcgccg actgcacgcg
     1621 ggtggcctgc ccggcccgct cgagcgcgtc ttgcagggcg agggcgttca tgacggtgcc
     1681 gagcatgccg atgtggtcgg actgcgtggc gtccatcccg gtcatcgcgc ccgtgcggcc
     1741 gcgccagaag ttgccaccgc cgacgacgac ggcaacgtcg acgcccaagt tctcacgcac
     1801 gtcgaggatc tcttgtgcgg tttgggccag cgttgggccg tcgaggcccg agccggacgg
     1861 accggcaagg gcctccccga cggtttgaag acgatccgct tccaccgggg cgaggccatc
     1921 gcaatcagcc gatctcgacc tgggcgaagc ggatgatgct cgccgagccg atcagctggg
     1981 tgatcgacac cttgtcgtct ttggcatacg gctggtcgag caaggcaacc tctttgaaga
     2041 agccctgcaa gcggccctcg atgatcttcg gcagcgccgc ttccggcttg ccttcgttgc
     2101 gcgagatcgt ttcgagggtg gcccgctcgg cggccaccac atcggccggg acgtcgtcct
     2161 tggagaggta cttcggcctg gcgaaggcga tgtgcaccgc cacgtcgtgg gccagttcgg
     2221 gcgttgcccc gctcatctcg agcagcacgg cgttgacgcc acggccaccc tgtacgtgca
     2281 ggtacgactc gagcaggttg ccctcggctg cgtcgaagcg gacgacctcg ccgatgctga
     2341 tgttctcctt gagcacgatc ttgaggtcct cgatcgatgc cgcccgctcg gcgacggcat
     2401 cggtgccctt gtgcacgacg aggccgacca gtgcgtcggc ttcggccttg aactggtcac
     2461 tgctggcgac gaagtccgtc tcgctcttga gcttgacgat gccaccgacg ttgccgtcga
     2521 tcagcagtgc gaccacgcct tgggcgctct cgcgctcgtc gcgcttggca gcagccgaca
     2581 gacccttctc gcgcagccac tgcttggccg cctcgacgtc gccatcgctg gcttcgaggg
     2641 ccttcttgca atccatcatc ccggcaccgg tggactggcg cagggcagag atgtccttgg
     2701 cagagatggt catgatcagc tctccggtga ttcggtggac tctgcgacgg gggccacagc
     2761 ttcgacaaca gcttcggccg cgacctcggt ctcggcggca acgtcggcct cgacggggac
     2821 atcggcgtcg gccgggttgg ccttggcagc ggtgaggcga gcgtcacgct cggcctgcgc
     2881 ggcagcagcc tgcttgcgag catcggcctg ggcggccatg tactcggcct cttcgtcggc
     2941 ggaacgcttg ggcggcgcgg tctgcggatt gcggcgcgag gcgatgtagc gaccctcttc
     3001 gatcgcttcc gcgatgaccc ggctcatcag cgcgttggcg cggatggcgt cgtcgttgcc
     3061 cgggatgggg tactggatta tgtcggggtc gacgttggtg tcgaccacgg cgatgatcgg
     3121 gatcttgagc ttgttcgcct cggtgacggc gatgtgctcc ttcttggtgt cgagcacgaa
     3181 caccgcgtcc ggacgctgcg ccatgccgcg gatgccgccg aggttcttct gcagcttctc
     3241 gagctcgcgg gtgagcagca gcgcttcctt cttcggcatg gcgtcgaatt cacccgacac
     3301 cttcatcc
//
LOCUS       DAORNF010000356         3300 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_21875_length_3300_cov_2.029584, whole genome shotgun sequence.
ACCESSION   DAORNF010000356 DAORNF010000000
VERSION     DAORNF010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3300
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21875_length_3300_cov_2.029584"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>252
                     /locus_tag="PLV68_13595"
     CDS             <1..>252
                     /locus_tag="PLV68_13595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HRE02331.1"
                     /translation="KPYGLVLVVGPTGSGKSTTLAAMIDRINQERPCHILTIEDPVEF
                     LHTHKTAMVNQREVGTDVASFPDGLRSALREDPDVVLLG"
     assembly_gap    253..346
                     /estimated_length=94
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <347..780
                     /locus_tag="PLV68_13600"
     CDS             <347..780
                     /locus_tag="PLV68_13600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type IV pili twitching motility protein PilT"
                     /protein_id="HRE02332.1"
                     /translation="ALDRIVDVFPAERRDQIQIQLAGALQGVISQRLVAVEGGGRVAA
                     YEVLMGTEAVRNLVREGKSRQMRNIITTSAKDGMQTIEMDLARLVASGLLSLEAACEV
                     SAYPKEIAAHTATVRAQMQAELTAMSGQAASTNGAPVAVGG"
     gene            846..1553
                     /locus_tag="PLV68_13605"
     CDS             846..1553
                     /locus_tag="PLV68_13605"
                     /inference="COORDINATES: protein motif:HMM:NF016163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF429 domain-containing protein"
                     /protein_id="HRE02333.1"
                     /translation="MPYKWLAGITPCPGGWLVVPARIAGITVIVEDAFVLKHLLDVVD
                     YRPKFDAAAINIPMGFSDEPNPNQLSECELQARGYVGWPRRVAVRPLVSRAALKAPTK
                     AQARAMEPWLTNDDFRKFRWLRQAADVFQPFHQRSFFSANPEVSFTLLNNDIPLKTSP
                     HHEDGVIERMDLIRKKLPGVEEVILRPIPDGAGRLHIVQAAGLVWTARRAIGHAVNRF
                     PLDPEWDTTGLRIEMVR"
     gene            complement(1595..1984)
                     /locus_tag="PLV68_13610"
     CDS             complement(1595..1984)
                     /locus_tag="PLV68_13610"
                     /inference="COORDINATES: protein motif:HMM:TIGR03618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HRE02334.1"
                     /translation="MSYPASHLDLLQTPPVVALSTITPKGAIQTTAVWYLLDDDGELR
                     VSLSDGRRKFHHLRANPTVNVFVVDPANPFRTLEIRGTAEIAPDSDGALRDKVGRKYG
                     ADLTAFDQPGETRWAITIRPDRVIPNG"
     gene            complement(2057..>2985)
                     /locus_tag="PLV68_13615"
     CDS             complement(2057..>2985)
                     /locus_tag="PLV68_13615"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HRE02335.1"
                     /translation="AGLEDVVISGGCEMMSLHPALGEVQLPLRGPLMDAGNLRLRASH
                     PQTHQGVAADAIASIEGISRDALDALAVESQVRAARAIEEGRFDRSLVTVYREDGSVA
                     LDREEFPRPGTTVEGLAKLAPSFAAMADVPLDSEGTTLRSMVLQRYPGLDITHVHHAG
                     NSSGVVDGAAALLMASEAAVQRNGWTPRARVVAAVNLGDDPTLMLNAPVPAARKVLER
                     AGLSIDDIDLFEINEAFAVVAEKFIRDLDLDRSKVNVNGGAIALGHPIAATGAMLIGT
                     VLDELERTGGRYGLVTMCAAGGMAPAIVIERL"
     assembly_gap    2986..3064
                     /estimated_length=79
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3065..>3300)
                     /locus_tag="PLV68_13620"
     CDS             complement(<3065..>3300)
                     /locus_tag="PLV68_13620"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377487.1"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HRE02336.1"
                     /translation="SDAFIIDACRTPRGIGKVGKGALADIHPQQLGATVLAAIRDRND
                     LDTATVDDIIFGTSMQAGTQGGDLARMAALDAGY"
BASE COUNT          505 a         1065 c         1073 g          484 t
ORIGIN      
        1 acaagccgta cggcctggtg ctggtggtgg ggccgaccgg ctccggcaag agcacgaccc
       61 tggcagcgat gatcgaccgc atcaaccaag agcggccgtg ccacattctc accatcgaag
      121 accccgtcga gttcctgcac acccacaaga cggcgatggt caaccagcgc gaggtcggca
      181 ccgacgtggc cagctttccc gacggcctgc gcagcgccct gcgtgaagac cccgacgtgg
      241 tgctgctggg cgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnaagc cctcgaccgc
      361 atcgtcgacg tgttccccgc cgagcgccgc gaccagatcc agatccagct cgccggtgcg
      421 ctgcaaggcg tgatcagcca acgcctcgtg gccgtcgaag gcggcggccg tgtcgcggcc
      481 tacgaggtgc tgatgggcac cgaggccgtg cgcaacctgg tgcgtgaagg caagagccgc
      541 cagatgcgca acatcatcac caccagcgcc aaggacggca tgcagacgat cgagatggac
      601 ctcgcccgcc tcgtcgcgag cggtctgctc agcctggaag cggcgtgcga ggtcagcgcc
      661 tatccgaagg agatcgcggc ccacaccgcc acggtccggg cccagatgca ggccgaactg
      721 accgcgatgt cgggccaggc tgcgtcgacc aacggcgcgc ccgtagccgt cggcggctga
      781 cccggtcgcc ctcatgccga caccgaccct gtactgatcg gaacccctcg atggtcgcca
      841 aacaaatgcc ttacaagtgg ttggccggga tcacaccgtg ccccggcgga tggctggtgg
      901 tgcccgcgcg catcgccggc atcaccgtca tcgtcgagga cgcgttcgtg ttgaagcatc
      961 tgctcgacgt ggtggactac cgccccaagt tcgacgcggc cgcgatcaac atcccgatgg
     1021 ggttcagcga cgagccgaac ccgaatcagc tcagcgagtg cgaactgcag gcccggggct
     1081 acgtcggctg gccacgccgc gtcgcggtgc gtccgctggt gtcacgcgcc gcgctgaagg
     1141 caccgaccaa ggcccaagcc cgggcgatgg agccatggct gaccaacgac gacttccgca
     1201 aattccgttg gctgcgccag gccgccgacg tgttccagcc gttccatcag cggtccttct
     1261 tctcggccaa ccccgaggtc agcttcacgc tgctcaacaa cgacataccg ctgaagacgt
     1321 cgccgcacca cgaagacggc gtgatcgaac ggatggacct gatccgcaag aagctgcccg
     1381 gtgtggaaga agtgatcctg cgcccgatcc ccgacggcgc cggccgcctg cacatcgtgc
     1441 aagcggcggg gctggtctgg accgctcgcc gcgcgatcgg tcacgcggtc aaccgcttcc
     1501 ccctcgaccc ggagtgggac accaccggtc tccgcatcga gatggtccgc tgacacgtcg
     1561 gcggttctct cgccgcgagc ggcgatcgag ttgatcagcc gttgggaatg acccggtcgg
     1621 gacggatcgt gatcgcccag cgggtttcgc cgggctgatc gaacgcggtc aggtcggcgc
     1681 cgtacttgcg gccgaccttg tcgcgcaacg cgccgtccga atcgggtgcg atctcggccg
     1741 tgccgcgaat ctccagcgtg cggaacgggt tggccggatc gaccacgaac acgttgaccg
     1801 tggggttggc ccgcaggtgg tggaacttgc gccgaccgtc gctgagcgag acgcgcagtt
     1861 cgccgtcgtc gtcgagcagg taccacacgg cggtggtctg aatcgcgccc ttgggggtga
     1921 tcgtgctcag cgcgacgacg ggcggggtct ggaggaggtc gaggtgggag gctgggtagc
     1981 tcatacgggt ggagtcaact cctgcagacc ccacccgtat tccagccgcg ccgatctatc
     2041 cggccggatc gaccgcctac aggcgttcga tgacgatcgc cggagccatc ccaccggccg
     2101 cgcacatcgt gaccagtccg taccggccgc cggtgcgctc caactcgtcg agaaccgtcc
     2161 cgatcagcat cgcgccggtc gccgcgatgg ggtggccgag cgcgatcgcg ccgccgttga
     2221 cgttgacctt gctgcggtcg aggtcgaggt cgcggatgaa tttctcggcc acgaccgcga
     2281 acgcctcgtt gatctcgaac aggtcgatgt cgtcgatcga cagcccggcc cgctccaaca
     2341 ccttgcgcgc cgctggcacc ggtgcgttga gcatcaaggt cgggtcgtcg ccgaggttga
     2401 ccgccgcgac cacgcgggcg cgcggggtcc agccgttgcg ttggaccgcg gcctcgctgg
     2461 ccatgagcag cgcggccgca ccgtcgacca cgcccgagct gttgccggcg tgatggacgt
     2521 gggtgatgtc caagccgggg tagcgctgca gcaccatgct gcgcagcgtg gtgccctcgc
     2581 tgtcgagcgg tacgtcggcc atcgccgcga acgacggggc gagcttggcc agcccttcca
     2641 ccgtcgtgcc cgggcgcggg aactcttcgc ggtcgagtgc gaccgaaccg tcttcgcggt
     2701 acacggtgac caggctgcgg tcgaagcggc cttcttcgat ggcacgcgcc gcacgcacct
     2761 gcgactcgac ggccagcgcg tcgagcgcgt cacgcgagat gccttcgatc gaggcgatcg
     2821 cgtcggccgc gacaccttgg tgcgtctgag ggtggctggc gcgtaggcgc aggttgccgg
     2881 cgtccatcag cggaccgcgc agcggcagtt ggacctcacc gagggccggg tggagcgaca
     2941 tcatctcgca gccgccggag atgacgacgt cttcgagacc ggccannnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnngtagcc ggcgtcgagt gccgccatgc gggccaggtc gccaccctgg gtgccagcct
     3121 gcatgctggt gccgaagatg atgtcgtcga ccgtcgcggt gtcgagatcg ttgcggtcac
     3181 ggatggcggc cagcaccgtg gcgccgagct gctgggggtg gatgtcggca agtgcaccct
     3241 tgccaacctt gccgatgcca cgtggtgtgc ggcaggcgtc gatgatgaat gcgtcggaca
//
LOCUS       DAORNF010000357         3282 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_22029_length_3282_cov_1.719244, whole genome shotgun sequence.
ACCESSION   DAORNF010000357 DAORNF010000000
VERSION     DAORNF010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3282
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22029_length_3282_cov_1.719244"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..962
                     /locus_tag="PLV68_13625"
     CDS             <1..962
                     /locus_tag="PLV68_13625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02337.1"
                     /translation="ACDYVFDARVSYVTEIKVVSSPAATGDNRVEPVCCFDFNDDGVV
                     DNKLGDILSLTIVRGQFNVNTTLEEQISEGGLVLLLETKNVTDVATSSNVTINGFYGA
                     DADADPSNNASGTASFVVNPSSFAPNTMSPVISFTGGAIASSTLNAGPSIFRLSIPLL
                     GASLDLAINGTRFESPVAAGPAGADKGLAMGGEDGGKLGGYVAMRDFATAFNTYLDAE
                     CGCIAKTDESKPFVSVTTGAAAESLSLNIKNGAACTGEDESTCKSVADALGTFGAILA
                     GFLTPDIDANGDGVKESLSIGVRLKATSAMVPNPGGVAECTAE"
     assembly_gap    978..987
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1989..2106
                     /estimated_length=118
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2107..>2541)
                     /locus_tag="PLV68_13630"
     CDS             complement(<2107..>2541)
                     /locus_tag="PLV68_13630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02338.1"
                     /translation="APFATFVATTLTATHGDHRNHARLTLTGGTLSQALTVGYQVRGQ
                     FSGGTTPWSTTANGRRATGTISRAWEYANLATPTTFATLPAIVCATSNTSCNDTAASP
                     LGVKRIYRATLSAPGATSLTTNTMEGWRLAFTYVDGGRFAMCA"
     assembly_gap    2542..2684
                     /estimated_length=143
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2685..>2930)
                     /locus_tag="PLV68_13635"
     CDS             complement(<2685..>2930)
                     /locus_tag="PLV68_13635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02339.1"
                     /translation="ALGYHIYRLGTRITPSQGVACASSPCLYADLDAPAAGAWVAPSS
                     VTATTNQTGGVTVSWNLPGRPKGTRQLYEVSAINPAG"
     assembly_gap    2931..3070
                     /estimated_length=140
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          458 a          950 c          943 g          520 t
ORIGIN      
        1 atgcgtgtga ctacgtcttc gacgctcgcg tgagctacgt gaccgagatc aaggtcgtca
       61 gctcgccggc ggccactggt gacaaccgcg tcgagcccgt ctgctgcttc gatttcaacg
      121 atgacggcgt cgtcgacaac aagctgggcg acatcctctc gctcacgatc gttcgcggcc
      181 agttcaacgt caacacgacc ctcgaggagc agatctccga ggggggcctg gtcctcctgc
      241 tcgagacgaa gaacgtcacc gacgtggcca cctcgagcaa cgtgacgatc aacggcttct
      301 atggcgcgga cgcggacgct gatcccagca acaatgcttc ggggacggcg tcttttgtcg
      361 tcaacccgag ctcgttcgct ccgaacacca tgagcccggt catctccttc accggtgggg
      421 ccatcgcgtc gagcacgctc aacgccggcc cgtcgatctt ccgcctcagc atcccgctgc
      481 tcggcgcgtc gctcgacctc gccatcaacg gcacccgctt cgagtcgccc gtggccgctg
      541 gcccggccgg cgcggacaag gggctcgcca tgggtggtga ggacggcggc aagctcggtg
      601 gctacgtggc gatgcgcgac ttcgcgacgg ctttcaatac gtacctggac gccgagtgcg
      661 ggtgtatcgc gaagacggac gagtcgaagc cgttcgtctc ggtcaccacc ggcgctgccg
      721 cggagtcgct ctcgctgaac atcaagaacg gcgcggcgtg cacgggcgag gatgagagca
      781 cctgcaagtc ggtcgcggac gcgctcggca ccttcggcgc gatcctcgct gggttcctca
      841 cgcccgacat cgacgcgaac ggcgacggcg tcaaggagtc gctctccatc ggcgttcgcc
      901 tgaaggccac ctcggcgatg gttcccaacc ccggcggtgt cgccgagtgc actgctgagt
      961 gatcctgggc ttgcgggnnn nnnnnnncgc ttgcaaccgc caagttgcga gcgcccgtcg
     1021 ctctagtgag acggacgaac actggcccac ctgaaataag tgagcttcag cgtctctcgg
     1081 cctaagagac tccggctatg ggagcgtaac gagggtcggg gctgcgatgt gcgttgtagt
     1141 cccgttccct cctgtcgcaa aagagttggc gccccagcac cacacgcctc cctcaacagt
     1201 cgcgcagatg gtgtcactcc ggccgccgag ctggttccat gaggcattgt tcagtgatgc
     1261 ctgaaccggc cacagggaat tttggaaagg caggccattg ccaagctggc cttggatgtc
     1321 ctgtccccaa caacgcacat gagcttcctg tatcgcgcag gttgtttccg atgttgcgag
     1381 cacttgggat gtgccttcca gcgcttcggt cgatgacgta aacacggccg cggggttgct
     1441 tttcgaagca gtcgtgccat cgccaagttg gccagcggtg tttgccccga agcagcgcag
     1501 ctggccgtta gcgaggcggg cgcacgtatg gtagctgccc agggcaagct caacgactcc
     1561 gctcaggttg gtgatctgag tagcggaatt gcgctgatct tggtctccga gcccgagctg
     1621 gccgtagttg ttgaaccccc aacaccagac acttcccgtt tctgttcgac cacaggcgtg
     1681 gtaaccgccc actgcgattt cgaccaaccc ggtcagggaa ctccctgtcg atctaacaac
     1741 cgtgacagga gttgcagcgt cgatggctct attgtcgccc agctgacctt cactgccctt
     1801 gccccagcag accgcttgcc cgttgctcct cactgcacag ttgcttctgt agccaatagc
     1861 gagtgctttc acgccggttc cgagatcggt tcgctccaca agttggccgt cctcccgaaa
     1921 cgagccgcag tacaccctgc cttggaggtt gcgcgcacac ccgaatcctg ctcctccacc
     1981 aaaggtcgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnngcgc acattgcgaa tcggccacca tcgacgtagg tgaaggccag gcgccagccc
     2161 tccatcgtgt tggtcgtgag cgacgtcgcg cccggcgcgg acagggtcgc gcggtagatc
     2221 cgcttgacgc cgagaggcga ggcggcggtg tcgttgcagc tcgtgttcga ggtggcgcag
     2281 acgattgccg gcaaggtcgc gaaggtggtg ggggtggcga ggttcgcgta ctcccaggcg
     2341 cggctgatgg tgccggtcgc gcggcggccg ttcgcggtcg tcgaccaggg cgtcgtcccg
     2401 cccgagaact ggccgcggac ctggtagccc accgtcaaag cctggctgag cgtgccgccc
     2461 gtcagcgtca ggcgcgcgtg gttgcggtgg tcgccgtggg tcgccgtgag cgtcgtcgcc
     2521 acgaacgtcg cgaagggggc gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncgcccg cggggttgat
     2701 ggcgctcact tcatagagct ggcgcgtccc tttgggccgc cctggcaggt tccagctcac
     2761 ggtgacgccg ccggtctggt tggtcgtcgc ggtcacgctg ctcggcgcga cccacgcccc
     2821 ggcggccggc gcgtcgaggt cggcgtacag acacggcgag ctcgcacagg caacgccctg
     2881 gctcggggtg atgcgggtcc ccagacggta gatgtggtag ccgagcgccc nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn cctcgcccgg taataacgga cgaccccgtt gggcgggatc ccctgcgagt
     3121 cgctgtcttc gtagttgcgc gcgctctgga tggggcccgc ggagccggcg atggccgcga
     3181 agtcgaccgg cgcgctggat gcggaacgct cccaggtgag cgtcacggcg cccgccttgc
     3241 gccgcccgct cgcgctgccg atggccccga ccccgaaacg gg
//
LOCUS       DAORNF010000358         3278 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_22060_length_3278_cov_1.888923, whole genome shotgun sequence.
ACCESSION   DAORNF010000358 DAORNF010000000
VERSION     DAORNF010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3278
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22060_length_3278_cov_1.888923"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>223)
                     /locus_tag="PLV68_13640"
     CDS             complement(<1..>223)
                     /locus_tag="PLV68_13640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HRE02340.1"
                     /translation="REDGDAELGDVVEDRSAESPFEVAATALLPDEISRLLAPLDERE
                     REILKLRFGLDRGEPRTLEEVGEHFNLTRE"
     assembly_gap    224..375
                     /estimated_length=152
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<376..848)
                     /locus_tag="PLV68_13645"
     CDS             complement(<376..848)
                     /locus_tag="PLV68_13645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRE02341.1"
                     /translation="MAKDRVDRDEEDLVRLYLSDIGQYTLLTKDDEVRLAKLIESGKA
                     AVAEMEAAGKEISALKKREYRRAAKEGEDAERAFVQSNLRLVVSIAKKYQASGLPLLD
                     LIQEGNLGLMHAVEKFDWRKGFKFSTYATWWIRQAITRGIANTGRTIRLPVHAGDT"
     gene            1069..1917
                     /locus_tag="PLV68_13650"
     CDS             1069..1917
                     /locus_tag="PLV68_13650"
                     /inference="COORDINATES: protein motif:HMM:NF024221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HRE02342.1"
                     /translation="MSNPILNDKAMREASQAGYAAGAWGPPQGAAGYAARETPITRAP
                     MTDGPISAWQSSSTVMTVQGATTATGVLFVVLLAAAALGWSMVKVSNGEIVSFPAWTI
                     GAILVGLGLVILMRFKPQWAKYIGFGYAVAQGLVVGAISHAYEVYQDGIVIQAIGATV
                     GVFAVMLFLYRTNVIKVTDRMRRIVMGATLGVAVFYGISLLINLFGGNVGFLTSTSLV
                     SVGFSFLVAGLAAFNLALDFDFIEKGSKQGLPKQMEWVAAVGLLVTVVWLYLEILRLL
                     SKLRDR"
     gene            complement(1963..2937)
                     /locus_tag="PLV68_13655"
     CDS             complement(1963..2937)
                     /locus_tag="PLV68_13655"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRE02343.1"
                     /translation="MNAEIRESMVELRHELGSVTLHVLEAGDPTNPTIVLSHGFPEAA
                     YSWRHQLPVLAAAGYHVVAPDQRGYGRSSVPSEVTDYGIRQLTGDLIALADHYGKDDA
                     VFVGHDWGALIVWELGRLYPQRARAIISVSVPAIEWLAPPTDVFKAVYQDNFFYMLYF
                     QAVGPAEAELGADIRRSVATVFWGASGEGWRPSGPVPKEGNGFMSQMPEPPALPWAWF
                     TEADLDYFTDHFQRSGFFGPVSYYRNMDANYGVMRGKGLDLLTMPTAFIAGKQDPVLL
                     MDSGGVERMQQSLPNFLGATIIDGAGHWTQQERPDEFNQALLALLARL"
     gene            complement(3005..>3278)
                     /locus_tag="PLV68_13660"
     CDS             complement(3005..>3278)
                     /locus_tag="PLV68_13660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02344.1"
                     /translation="ADATSIVHSLASPDVSDDVSAAPPTAAAAAIDLDVPDLHVPDLD
                     VIERDLADVEIALSRLEAGTYWTCEDTGQPLPDALLAARPTARTIH"
BASE COUNT          494 a         1006 c         1074 g          552 t
ORIGIN      
        1 ctcgcgggtg aggttgaagt gctcgccgac ctcttcgagc gtgcgcggct cgcctcggtc
       61 gagaccgaag cgcagcttga ggatctcgcg ctcgcgctcg tcgagcggag cgagcaggcg
      121 gctgatctcg tcgggcagca gcgcggtggc ggcgacctcg aacggcgact cggccgaacg
      181 gtcttcgacc acgtcgccca actcggcatc gccgtcttca cgcnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnngtgtc gcccgcgtga acaggcaggc gaatggtgcg gcccgtgttg
      421 gcgatgccgc gggtgatcgc ctggcgaatc caccacgtgg cgtaggtgga gaacttgaag
      481 cccttgcgcc agtcgaactt ctcgaccgcg tgcatcaggc cgaggttgcc ctcttggatc
      541 aggtcgagca gaggcaggcc cgaggcctgg tacttcttgg cgatcgacac caccaaacgc
      601 aggttggact gcacgaaggc acgctcggcg tcttcgccct ccttggcagc ccggcggtac
      661 tcgcgctttt tgagcgccga gatctccttg ccagcggctt ccatctcggc cacggcggcc
      721 ttgcccgact cgatgagctt ggccaggcgt acctcatcgt ctttcgtgag cagtgtgtac
      781 tgcccgatgt cagacagata gagccggacg aggtcctcct catcgcggtc gacgcgatct
      841 ttggccacgg gtgctcctca ggatgtcgag tcggggttgt cgacgcctga accgcgaaag
      901 acgcgttcca ggctgggcga tgtggtaacg ataccgacga cgtcaaggcc ccacacgggg
      961 ccctccggtt ttgtctgcgg ggccctcgaa gtggcacgca gccgaccgac gccaagagtg
     1021 tcgatgtatg gctgtggcaa atcggctcgg cgcactagcg ttcagtgcat gtccaatccg
     1081 attctgaacg acaaggcgat gcgcgaggcc tctcaggccg ggtacgccgc cggagcatgg
     1141 ggcccgccgc aaggcgccgc cgggtacgcg gcgcgcgaga ccccgatcac gcgcgccccg
     1201 atgaccgacg gcccgatcag cgcgtggcag tcgtcgtcca ccgtcatgac ggtgcagggt
     1261 gccaccacgg ccacaggcgt gttgttcgtg gtgctgctgg ccgcggcggc cctcggctgg
     1321 agcatggtca aggtcagcaa cggcgagatc gtgtccttcc ccgcctggac gatcggggcg
     1381 atcctggtcg gtctcgggtt ggtcatcctg atgcgattca agccccagtg ggccaagtac
     1441 atcgggttcg gttacgcggt cgcgcagggc ctggtggtcg gtgcgatctc ccacgcctac
     1501 gaggtgtacc aagacggcat cgtcatccaa gcgatcggcg ccaccgtcgg cgtgttcgcg
     1561 gtgatgctgt tcctctaccg caccaacgtc atcaaggtca ccgaccgcat gcgccgcatc
     1621 gtgatgggcg cgaccctcgg tgtggccgtg ttctacggca tcagcctgtt gatcaacctg
     1681 ttcggtggca acgtcggctt cctcaccagc accagcctgg tcagcgtcgg cttcagcttc
     1741 ctggtcgcgg gtctggctgc gttcaacctg gcgctcgact tcgacttcat cgagaagggt
     1801 tccaagcagg gcttgcccaa gcagatggag tgggtcgcgg ccgtcggcct gctggtgacg
     1861 gtggtctggc tgtacctcga aatcctgcgc ctgctctcca agctgcgcga ccgctgacct
     1921 ggccgccagc cgcccgccgc cagggcggtc gcggacggtc cgctacaacc tggcgagaag
     1981 tgccagcagc gcctggttga actcgtcggg gcgctcttgc tgggtccagt gcccggcgcc
     2041 gtcgatgatg gtggcgccca agaagttggg cagtgactgc tgcatccgct cgactccgcc
     2101 gctgtccatc agtagcaccg ggtcttgctt gccggcgatg aacgcggtcg gcatcgtcaa
     2161 cagatcgagg cctttgccac gcatcacgcc gtagttggcg tccatgttgc ggtagtagct
     2221 gaccggaccg aagaagccgc tgcgctggaa gtggtcggtg aagtagtcga ggtcggcctc
     2281 ggtgaaccac gcccacggca gcgcgggcgg ctcgggcatc tggctcatga agccgttgcc
     2341 ctccttcggc accggcccgc tcgggcgcca accctcgccg ctggcacccc agaacaccgt
     2401 ggccacggag cggcggatgt cggcaccgag ctcggcttcg gccggtccga ccgcttggaa
     2461 gtacagcatg tagaagaagt tgtcctgata gacggctttg aacacgtcgg tcggaggagc
     2521 gagccactcg atcgcgggca cgctgacgct gatgatcgcc cgggctcgtt gcgggtacag
     2581 gcggccgagc tcccacacga tcagcgctcc ccaatcgtga ccgacgaaca cggcgtcgtc
     2641 cttgccgtag tggtctgcaa gcgcgatcag atcaccggtc agttggcgga tgccgtagtc
     2701 ggtgacctcg gacggcacgg aggagcgccc gtatccgcgc tggtcagggg cgacgacgtg
     2761 gtagccggcg gccgcgagca ccggcagctg gtggcgccag gaataggcgg cttcgggaaa
     2821 gccgtggctc aacacgatcg tcgggttggt gggatcgccc gcctccagca cgtgcaacgt
     2881 gaccgaaccc aactcgtgtc tgagctcgac catcgactcg cggatctctg cgttcatgac
     2941 cggcagtctc gcgaactgcg ggcaccacaa ccgtcagcgg agcgaccccc ggtgggggca
     3001 gccgtcagtg gatcgtgcgc gcggtggggc gagcggccag gagagcgtcg ggcagcggct
     3061 ggccggtgtc ttcgcaggtc cagtaggtgc ccgcctccag gcggctgagc gcgatctcga
     3121 cgtcggccag gtcgcgctcg atgacgtcga gatcgggaac gtggagatcg gggacgtcga
     3181 gatcgatcgc tgccgcagca gcggttggcg gtgctgcact gacgtcgtca ctcacgtccg
     3241 gcgaggctag cgagtgaaca atcgatgtcg cgtcggcg
//
LOCUS       DAORNF010000359         3270 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_22144_length_3270_cov_1.231726, whole genome shotgun sequence.
ACCESSION   DAORNF010000359 DAORNF010000000
VERSION     DAORNF010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3270
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22144_length_3270_cov_1.231726"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>300
                     /locus_tag="PLV68_13665"
     CDS             <1..>300
                     /locus_tag="PLV68_13665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HRE02345.1"
                     /translation="EEVLLQPPVGEVLPEGGYDPGQNTFTPPPADSSDVVVEVSPTSS
                     RLQLLQAFSPWDGNDYLNLPVLMKAQGKCTTDHISAAGKWLTYRGHLENISGNLFL"
     assembly_gap    301..544
                     /estimated_length=244
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <545..803
                     /locus_tag="PLV68_13670"
     CDS             <545..803
                     /locus_tag="PLV68_13670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HRE02346.1"
                     /translation="KKQGLVPLTFADSDTYELIGEDDLINVFNLPPVPGQNVRCQIVK
                     PDGTLVDFEAKHTFSDEQVEWFKAGSALNVVRKKVAEAAAR"
     assembly_gap    935..1005
                     /estimated_length=71
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1006..>1330)
                     /locus_tag="PLV68_13675"
     CDS             complement(<1006..>1330)
                     /locus_tag="PLV68_13675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02347.1"
                     /translation="TDLGDVARQMVGSLGWLEFSAPWVAQAMWWALLVGAAIGVASGA
                     RGGQRRLLGAWGCVVAITVVTPVVFETVFAGRVGFIWQGRYSIPTALGAVVFGVGTWS
                     RCLSRR"
     assembly_gap    1331..1381
                     /estimated_length=51
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1382..>1675)
                     /locus_tag="PLV68_13680"
     CDS             complement(<1382..>1675)
                     /locus_tag="PLV68_13680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02348.1"
                     /translation="NPNSIEIALVLLAWVGAERARLAGSIARADAWWIAAPLAAAVVI
                     RPVAAVAAAAVIVALAVSVRTPGRRLTRGLRPVVVAVLAAAGAAVAWNVVVGVA"
     assembly_gap    1676..1693
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1694..2208)
                     /locus_tag="PLV68_13685"
     CDS             complement(<1694..2208)
                     /locus_tag="PLV68_13685"
                     /inference="COORDINATES: protein motif:HMM:NF021436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2142 domain-containing protein"
                     /protein_id="HRE02349.1"
                     /translation="MARRDAARVTALFGLLWLVIASWSLATARYGGPDEPAHVLRAAS
                     VARGDVVGDPIDWLPPGYRMVTVAAGLTTGDPTCYRHNAALPATCAHADPAATGLGEA
                     GSSAGTYPPYSYALVGLPARLVGGGGSVLAHRVIAAAWCAAILAVAFWRARRIAGPLL
                     ITAVMPAAWFL"
     gene            complement(2181..2606)
                     /locus_tag="PLV68_13690"
     CDS             complement(2181..2606)
                     /locus_tag="PLV68_13690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02350.1"
                     /translation="MTPGPPTKIHVLYTGLNKAMIVLGACRANSWIEVDDDTVTVRMA
                     WWFRARFASASLIGAAPDARRVLCRGVHGWAGRWLVNGSSKNIVRVDVEPPARAYVLG
                     VPVRLRCLRISVEDPQALIAAVEAIAPHSARWRAATPHG"
     assembly_gap    2796..2826
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2827..>3270
                     /locus_tag="PLV68_13695"
     CDS             <2827..>3270
                     /locus_tag="PLV68_13695"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02351.1"
                     /translation="ALMQAVWNEYRAAEAANGADTWLAPFAVIGADGTPTLPSSGEAA
                     TSAPFVPNTLLDHIAEIPVMLVVAADLRCIAMMDAHLDRVPLTGGASIYPFCWNLLLA
                     ARARGLGGVLTTFLARAEREAAADVAALSAEVAALRDEVATLRAGA"
BASE COUNT          504 a         1054 c          931 g          366 t
ORIGIN      
        1 cgaagaggtc ctgctgcagc ctccggtcgg cgaggtgctc cccgaggggg gctacgaccc
       61 gggtcagaac accttcactc ccccaccggc cgattccagc gacgtcgtgg tcgaggtgtc
      121 gcccaccagc tcgcggctgc aactgctcca ggcgttctcg ccgtgggatg gcaacgacta
      181 cctcaacctg ccggtgttga tgaaggccca gggcaagtgc accaccgacc acatttcggc
      241 cgctggtaag tggctcacct atcgcggtca tctcgagaac atcagcggca acctgttcct
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      541 nnnngaagaa gcagggcctg gtgccgctga ccttcgccga ctccgacacc tacgaactga
      601 tcggtgagga cgacctgatc aacgtgttca acctgcctcc ggtgccgggc cagaacgtgc
      661 gctgccagat cgtcaagccc gacggcacgc tggtcgactt cgaagccaag cacaccttca
      721 gcgacgagca agtcgagtgg ttcaaggccg gctcggccct caacgtggtg cgcaagaagg
      781 ttgccgaggc cgccgctcgc tgacggtgac gagtgccgtc gcggcgatca acgcgcccgt
      841 cgctgtcacc agcacgaccc acggtgacag cggcggatgc cacgccgcgt cgcgtagcca
      901 ccagcttcca tcgacgccga cgctgtaccg gcgcnnnnnn nnnnnnnnnn nnnnnnnnnn
      961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncccgc cgtgacaagc
     1021 agcggctcca cgtaccgacg ccgaagacga cggcacccaa cgccgtgggg atgctgtagc
     1081 ggccttgcca gatgaacccg acccgtccgg cgaacaccgt ctcgaacacg acgggtgtga
     1141 cgacggtgat cgcgacgaca catccccacg ccccgagcag cctgcgctga ccgccgcgtg
     1201 cgccagacgc caccccgatc gcggccccga cgagtagcgc ccaccacatg gcctgggcga
     1261 cccacggggc ggagaactca agccatccga gcgagcccac catctggcgg gcgacgtcgc
     1321 ccagatcggt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 ncgcgacacc gaccaccacg ttccaggcca ctgccgcacc agcagcggcc aagacggcga
     1441 ccacgacagg tcgcaacccg cgcgtcaacc ggcgcccggg cgtgcgtacc gacaccgcca
     1501 acgcgacgat cacggctgct gcggccaccg cggcgacagg gcgaatcacg accgctgcgg
     1561 ccagtggggc cgcgatccac catgcgtcgg cacgggctat cgaacccgcg agccgcgccc
     1621 gttcagcacc gacccaggcc agcagcacca gagcgatctc gatcgagttg gggttnnnnn
     1681 nnnnnnnnnn nnnaacagaa accacgccgc gggcatcacc gccgtgatca gcagcggccc
     1741 ggcgatgcgc cgggcgcgcc agaaggccac ggccaggatg gccgcacacc acgcggcggc
     1801 gatgacccgg tgggccaaca ccgatcctcc cccaccaacc agacgagcgg gcaggccgac
     1861 gagcgcgtag gagtacggcg ggtaggtccc agccgacgaa ccggcctcgc cgagacccgt
     1921 cgcggcaggg tcggcgtggg cgcaggtcgc gggcagggct gcgttgtggc ggtagcaagt
     1981 cgggtcaccg gtcgtcagcc ccgcggccac ggtgaccatc cggtagccgg gtggcagcca
     2041 gtcgatcgga tcaccgacga cgtcgccgcg cgcgaccgac gccgcgcgca acacatgggc
     2101 cggttcgtcc ggtcccccgt agcgcgctgt cgccagcgac cacgacgcga tcaccaacca
     2161 cagcaggccg aacagcgccg ttacccgtgc ggcgtcgcgg cgcgccatcg tgctgaatgg
     2221 ggggcgatag cttcgacggc agcgatcagc gcctgtgggt cctcgaccga gatgcgcagg
     2281 caacgcaggc gaaccggtac gccgagcacg tatgcccgcg ccgggggctc gacgtcgact
     2341 cgcacgatgt tcttggacga gccgttgacc aaccatcgcc cagcccaacc gtggacgccc
     2401 cggcacagca ctcggcgcgc atccggcgcg gcaccgatca gcgaggccga cgcaaacctg
     2461 gcccgaaacc accacgccat ccgcaccgtg accgtgtcgt cgtcaacctc gatccaactg
     2521 ttggctcgac acgcgcccaa cacgatcatc gccttgttga gaccggtgta cagcacgtgg
     2581 atcttggtcg gcgggcctgg cgtcatgacg gcatcgtaga cacagcgggc atcgtcggca
     2641 gggctcacta gggtgcagcc gatggaactg cgcgaggcaa tccgtggcac gggctccgtg
     2701 cgcaccttca ccgaccagcc ggtcgacgac gacacgctgg cagcggtact cgacgacgct
     2761 cgcttcgccc ccagcggcgg aaaccgccaa ggctgnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnngcgt tgatgcaggc cgtgtggaac gagtaccgcg ccgccgaggc cgccaacggg
     2881 gccgacacgt ggctggcacc gttcgccgtc atcggcgccg acggcactcc gacgctgcca
     2941 tcctccggcg aggccgccac ctcggcaccg ttcgtaccga acacgttgct cgaccacatc
     3001 gcggaaatcc cggtgatgct cgtcgtcgcg gccgacctac ggtgcatcgc gatgatggac
     3061 gcccacctcg accgggttcc gctcaccggc ggcgcgtcga tctacccgtt ctgctggaac
     3121 ctgctcctcg ccgctcgcgc ccgcgggctg ggtggcgtgc tgacgacgtt tctcgcccgc
     3181 gccgaacggg aggcggccgc tgatgtggcc gcgctgagcg ccgaagtggc agcgctgcgt
     3241 gacgaggtcg ccacgctgcg cgccggcgcc
//
LOCUS       DAORNF010000360         3253 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_22291_length_3253_cov_1.984678, whole genome shotgun sequence.
ACCESSION   DAORNF010000360 DAORNF010000000
VERSION     DAORNF010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3253
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22291_length_3253_cov_1.984678"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..615)
                     /locus_tag="PLV68_13700"
     CDS             complement(<1..615)
                     /locus_tag="PLV68_13700"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HRE02352.1"
                     /translation="MTAPRAAWRWSTGWPAPTRCSPASPRRSKSAVDEASLVRPRWAH
                     GRTVAAHAAGHVVAEMHAAIRAAAAPGVTTAALDQVARDVLVRCDATSNFLGYHGFPA
                     VICASVNDEVVHGLPGDRPLADGDLLSIDCGAVVHGWHGDAAFSMVIGGADANPAAAQ
                     LIAAAERALAAGIAAMVPGGHVGDIGAAIDSVVRAAGYGRPRDYC"
     gene            complement(515..1174)
                     /locus_tag="PLV68_13705"
     CDS             complement(515..1174)
                     /locus_tag="PLV68_13705"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HRE02353.1"
                     /translation="MIPGARLIILGRQGAGKGTQCVRLARHYVVPHISTGDMLRAAVR
                     EGTPLGRAAKVVIEAGRLVSDEIMVGVVTERLARPDARVRGYILDGFPRTVGQAQALD
                     DITVDRPIDVVIDLDVPRALVLERLSARRLCGDCGTNYVAKGGERQPWLCDVCGGDVV
                     QRADDTPEAINQRLDLYEQQTKPLIEYYGASGRLAVVDGVASPDEVFTRLTAAVEVSR
                     R"
     gene            complement(1179..>2283)
                     /gene="secY"
                     /locus_tag="PLV68_13710"
     CDS             complement(1179..>2283)
                     /gene="secY"
                     /locus_tag="PLV68_13710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HRE02354.1"
                     /translation="VFALGIMPYITASIIMQVLGVVIPKLEQLQQQGAVGQRKITQYT
                     RYLGIGIALLQAAGLTFIFGQGSGSAFFGGAQNPPDVQLLPEFMPRALLVIITLVAGT
                     ALVMWMGELISQRGIGNGMSMIIFASVVSSLPYSFARIWQEESKVLFVLLCVLVVAIV
                     LAVVAIELGVRRIPVSFAKRQVGRRMYGGQNTYIPLKVNQSGVIAIIFANSVLLLPVL
                     VTNVLGSGEGWRGWITDKVNRYIVNSNNAGYAILLFLLVIAFSYFYNSIAFDPIRQAD
                     QIRKQGGFIPGIRPGPQTERYLGKVVNRITLPGAVFTGIIAILPLALLAAFGVQNFPF
                     AGTTVLIAVGVALELMRQIDSQLTLRNYEGFLK"
     assembly_gap    2284..2298
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2299..2505)
                     /locus_tag="PLV68_13715"
     CDS             complement(<2299..2505)
                     /locus_tag="PLV68_13715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HRE02355.1"
                     /translation="MPVLSSVKNVFKVADLRNKILFVLLIVALYRFGVAIQVPGVDQH
                     AVEQLRDSAEGGGALGFLNLFSGGA"
     gene            complement(2576..3067)
                     /gene="rplO"
                     /locus_tag="PLV68_13720"
     CDS             complement(2576..3067)
                     /gene="rplO"
                     /locus_tag="PLV68_13720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HRE02356.1"
                     /translation="MRVDELAPAPGSNRRPKRVGRGIGGKGGKTAGRGTKGQHARGRG
                     KVARGFEGGQMPLKQRVPKLKGFNNPFRVEYTAINFHAIERAGLTEVTPETLVAAGLL
                     RKGDYAKVLAYGELTTAINVSVHAVSKTAAEAIAARGGSVTIIPMPFTVRPAANGNQF
                     TNR"
     gene            complement(3067..>3253)
                     /gene="rpmD"
                     /locus_tag="PLV68_13725"
     CDS             complement(3067..>3253)
                     /gene="rpmD"
                     /locus_tag="PLV68_13725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HRE02357.1"
                     /translation="AHLVVTQKRSSIGAKPKTRATLRGLGLRKIGDTNVLPDRPEIRG
                     MIARVPHLISVEERTAN"
BASE COUNT          567 a         1069 c         1098 g          504 t
ORIGIN      
        1 gcaatagtca cgcggcctgc cgtagccggc cgcgcgcacc accgagtcga tcgccgcgcc
       61 gatgtcgccg acatgaccgc ccggcaccat cgccgcgatc ccggccgcca gcgcacgctc
      121 ggccgccgcg atcagttggg ctgcggccgg gttggcatcc gcgccgccga tcaccatcga
      181 aaatgccgca tcaccgtgcc agccgtggac gacggcaccg cagtcgatcg acagcagatc
      241 gccgtcggcc aggggccggt cgccggggag gccgtggacg acctcgtcgt tgacggacgc
      301 gcagatcacg gccgggaagc cgtggtagcc gaggaaattc gacgtcgcgt cgcagcggac
      361 gagcacgtcg cgcgccacct ggtcgagcgc cgcggtggtg acacccggcg cggctgcggc
      421 ccgaattgcg gcatgcatct cggccaccac gtggcccgcc gcatgtgccg caactgttcg
      481 gccgtgcgcc caccgaggac gaacgagcga cgcttcatcg acggctgact tcgaccgccg
      541 cggtgaggcg ggtgaacacc tcgtcggggc tggccacccc gtcgaccacc gccaggcggc
      601 ccgaggcgcc gtaatactcg atcagcggct tggtctgctg ctcgtacagg tcgaggcgtt
      661 ggttgatcgc ctcgggggtg tcgtcggcac gctgcaccac atcgccaccg cacacgtcgc
      721 acagccacgg ctgacgctcg ccacccttgg cgacgtagtt cgtgccacag tcgccgcaca
      781 gccgccgtgc cgagagccgc tcgagcacca gcgcacgcgg cacgtcgagg tcgatcacca
      841 cgtcgatcgg ccggtcgacg gtgatgtcgt cgagcgcttg ggcctggccg accgtgcgcg
      901 gaaagccgtc gaggatgtaa ccccgcaccc gggcgtcggg ccgggcgagg cgttcggtga
      961 cgacaccgac catgatctcg tcgctgacca gccgaccggc ctcgatgacc accttggccg
     1021 cgcgccccag cggcgtgcct tcacgcaccg cggcgcgcag catgtcaccg gtggagatgt
     1081 gcggcaccac gtaatgacgc gcaaggcgca cacattgtgt ccccttgcca gccccttggc
     1141 ggccgaggat gatgagccga gcccccggaa tcatcaggtc acttcaggaa gccctcgtag
     1201 ttacgcaagg tgagctgact gtcgatctga cgcatcaact cgagcgcaac accaacggcg
     1261 atcagcacgg tggtgccggc gaaggggaag ttctgcacgc cgaaggcggc caggagcgcc
     1321 aagggcagga tggcgatgat gccggtgaac acagcaccgg gcagggtgat gcggttgacg
     1381 accttgccga ggtagcgctc ggtctggggg ccaggacgga tgccggggat gaagccaccc
     1441 tgcttgcgga tctggtcggc ctggcggatc gggtcgaagg ctatcgagtt gtagaagtac
     1501 gagaaggcga tcaccagcag gaacagcaag atcgcgtagc ccgcgttgtt gctgttgacg
     1561 atgtagcggt tgaccttgtc ggtgatccag ccacgccagc cctcgcccga gccgagcacg
     1621 ttggtgacca gcaccggcag cagcagcacc gagttggcga agatgatcgc gatcacgccg
     1681 ctctggttga ccttgagcgg gatgtaggtg ttctggccgc cgtacatccg ccggccgacc
     1741 tggcgcttgg cgaacgacac cgggatgcga cgcacgccca gttcgatcgc cacgacggcc
     1801 agcacgatgg cgaccaccaa cacgcagagc agcacgaaca ggaccttgct ctcctcttgc
     1861 cagatgcgag cgaagctgta gggcaggctg gacacgaccg acgcgaagat gatcatcgac
     1921 atgccgttgc cgataccccg ctggctgatc agctcaccca tccacatcac cagcgccgtg
     1981 ccggcgacca gggtgatgat gacgagcaag gcgcgcggca tgaactcggg cagcaactgc
     2041 acgtcgggcg ggttctgtgc accgccgaag aaggcgctgc cgctgccctg gccgaagatg
     2101 aacgtcagac cagcggcctg caacagggcg atgccgatgc ccaggtaacg ggtgtactgg
     2161 gtgatcttgc gctggccgac cgcaccttgt tgttgcagct gttcgagctt ggggatcacc
     2221 acaccgagca cctgcatgat gatgctggcg gtgatgtacg gcatgatgcc cagggcgaac
     2281 accnnnnnnn nnnnnnnnag ccccacccga gaacaggttg aggaatccga gcgctccgcc
     2341 gccttcggcc gagtcacgca actgctcgac ggcatgctgg tcgacgcctg gcacctggat
     2401 cgccacaccg aagcggtaca aggcgacgat caacagaacg aacaggatct tgttccgaag
     2461 gtccgcaacc ttgaagacgt tcttcacgct ggacagaact ggcacgagca aactcctcgg
     2521 tgtgcagtga ttcggccgtg caacctaccg gccgcgacgg ccggcgtgga agaaatcagc
     2581 ggttggtgaa ctggttgccg ttggccgcag ggcggaccgt gaacggcatc ggaatgatgg
     2641 tgacgctgcc accgcgggcg gcgatggctt cggcggccgt cttcgacacc gcgtgcaccg
     2701 agacgttgat cgccgtggtg agctcgccgt acgcgagcac cttggcgtag tcgcccttgc
     2761 gcagcaagcc ggctgccacc agcgtctcgg gggtcacctc ggtgagcccg gcgcgctcga
     2821 tggcgtggaa gttgatcgcc gtgtactcga cgcggaacgg gttgttgaac cccttcaact
     2881 tcggcacgcg ctgcttgagg ggcatctggc cgccttcgaa gccgcgggcg accttgccac
     2941 ggccgcgggc gtgttggccc ttggtaccgc ggccagcggt cttgccgccc ttgccgccga
     3001 tgccacggcc gacccgcttg gggcggcggt tggacccggg tgctggagcg agttcgtcga
     3061 cgcgcatcag ttcgccgtcc tttcttcgac cgaaatgagg tgcggcacgc gtgcgatcat
     3121 gccacggatc tcggggcgat cgggcagcac attggtgtcg ccgatcttgc gcagtccgag
     3181 gccacgcagg gtggcccgag tcttgggctt ggcaccaatg ctcgaccgct tctgcgtgac
     3241 gacgaggtgc gct
//
LOCUS       DAORNF010000361         3249 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_22325_length_3249_cov_1.600814, whole genome shotgun sequence.
ACCESSION   DAORNF010000361 DAORNF010000000
VERSION     DAORNF010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3249
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22325_length_3249_cov_1.600814"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="PLV68_13730"
     CDS             complement(<1..417)
                     /locus_tag="PLV68_13730"
                     /inference="COORDINATES: protein motif:HMM:NF019826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HRE02358.1"
                     /translation="MRIATLAKTFRVTTETARRDLDELDNTRTGSNRSQLTFDVTKVV
                     KVTLDHFFGIEIEEWPARIAETAMLLVDHLANQRMEEEFGLAPDRLPIVLAPTIEHGN
                     ALDVAWERVLPPSEHVIVVGNPPFIGNKRMRFNLGDG"
     assembly_gap    431..440
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<441..>794)
                     /locus_tag="PLV68_13735"
     CDS             complement(<441..>794)
                     /locus_tag="PLV68_13735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02359.1"
                     /translation="VEFRYINGGLFGDELRLPKLSPGFRAGLLSACDFDWQMISPAVF
                     GSMFQTIKDKEARRHGGEHYTTEENILKTIRPLFLDELTERLDAAWDDKGQLTRLHNY
                     MGELRFLDPACGCGN"
     assembly_gap    795..876
                     /estimated_length=82
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<877..>1274)
                     /locus_tag="PLV68_13740"
     CDS             complement(<877..>1274)
                     /locus_tag="PLV68_13740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02360.1"
                     /translation="QIIDAQQRLMGALALGAQGGVNAKLLMHRHIVGQPDTHLDQQLR
                     AIEIDLDADITLVDLLSRMRAAFDVEAESASVQAARHMKLLYDELEKSKVSADEATLL
                     LARLLFLLFGDDSGMWKKDLLHTYLVEHTT"
     assembly_gap    1275..1284
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1285..1545)
                     /locus_tag="PLV68_13745"
     CDS             complement(<1285..1545)
                     /locus_tag="PLV68_13745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02361.1"
                     /translation="MTSQLLGLVDAADFRQLFIDQLGWNNPDKPDLHLTVDDKTYVLK
                     QMAGYRGLRVWCCDELPERKVQRELDRMVGKDNLERLVIFVGS"
     gene            complement(1542..2966)
                     /locus_tag="PLV68_13750"
     CDS             complement(1542..2966)
                     /locus_tag="PLV68_13750"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C-terminal helicase domain-containing protein"
                     /protein_id="HRE02362.1"
                     /translation="MDDEDVTDDDAADGELAAGSPATRYAALLSANPAGLRWVRPDLF
                     DDALRADLDTDTSQLQSLLDWYGTWAPDRDSKLAALIELVAKTHPNEKVLVFTEYRDT
                     ATYVAAQLEAAGVDGVGLATGETENPTAVAHRFAPVSSALPGQDVEMPANELRVLVAT
                     DVLSEGQNLQDAHIVVNYDLPWAIIRLIQRAGRIDRIGQTSHEVRVYSFFHESVDDVI
                     ALRQRIADRLAANAEAFGSDERFFGTVGETQVIVDLYNGQLDDVDDGGEVDASSMAYQ
                     YWKNLEDNDPDLARRIAALPDLLDATRSRRASESEDGVACYVRTHSGVDGFGFAALDG
                     TTRLLTGHEALRVFEAPPDEPGRPLRDDHDVLVRELVQGPLSAPTVLAGRLRGVRRAI
                     WQRLGETLQAHDGDTSNALEALYQHPLTSEADRRLRRAIRNGISDDDLSARIAALHRD
                     DALVVEVRTGADPVRIVSTMGVAQ"
BASE COUNT          548 a          993 c         1007 g          599 t
ORIGIN      
        1 cccatccccc agattgaatc gcatccgctt attcccgatg aacggcgggt tgcccacgac
       61 gatgacatgc tcggacggcg gcaggacgcg ctcccacgcg acgtcgagcg cgttgccgtg
      121 ctcaatcgtc ggcgcgagca cgatcggcaa gcggtcgggc gccagcccga actcctcttc
      181 catccgctgg ttggcgagat gatccacgag cagcatcgcc gtctcagcga tgcgtgcggg
      241 ccattcttcg atctcgattc caaagaagtg gtcgagcgtg accttgacga ccttggtcac
      301 gtcgaacgtc agctgcgagc gattgctgcc agtcctcgtg ttgtcgagtt cgtccaggtc
      361 gcggcgtgcc gtctcggtgg tcacgcggaa cgtcttcgcg agcgtcgcga tgcgcacgac
      421 gccgtcgcgc nnnnnnnnnn aagttgccac agccgcaggc cgggtcgagg aaccgcagct
      481 cgcccatgta gttgtgtagg cgggtgagct ggcccttgtc atcccatgcc gcgtcgagcc
      541 gttcggtcag ctcgtctagg aacagtgggc ggatggtctt gaggatgttt tcctcggttg
      601 tgtagtgctc gccaccgtgc cgccgagctt ccttgtcctt aatggtctgg aacatcgagc
      661 cgaacacggc tggcgagatc atctgccagt cgaagtcgca ggcgctgagc agcccggctc
      721 ggaacccggg ggagagcttg ggcaggcgga gttcgtcacc gaacaggccg ccgttgatgt
      781 agcggaactc gaccnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncggt ggtgtgctcg accaggtagg
      901 tgtggagcag atccttcttc cacatcccgc tgtcgtcacc gaacaacagg aacagcaagc
      961 gagccaggag caacgtggcc tcgtcggcac tgaccttgga tttctccaac tcgtcgtaga
     1021 gcagcttcat gtgacgagcg gcttggaccg aggccgactc ggcttcgaca tcgaaagccg
     1081 cccgcatccg actgagcagg tccaccaacg tgatgtcggc atcgaggtcg atctcgatgg
     1141 cccgcaactg ttggtcgagg tgggtgtcgg gctggccaac gatgtgacgg tgcatcagga
     1201 gcttggcgtt taccccgccc tgcgcgccga gcgcaagcgc gcccatcagg cgttgctggg
     1261 cgtcgatgat ctggnnnnnn nnnnactgcc aacgaagatg accagtcgct caaggttgtc
     1321 cttgccgacc attcgatcca gctcgcgttg caccttgcgc tcgggcaact cgtcgcaaca
     1381 ccagacccgc agacctcggt agcctgccat ctgcttgagt acataggtct tgtcatcgac
     1441 cgtcaggtgc agatcgggct tgtcggggtt gttccatccg agctggtcga tgaagagctg
     1501 tcggaagtcc gcggcgtcta cgagaccgag cagctggctg gtcattgggc tactcccata
     1561 gtgctcacga tcctcaccgg gtcagcgccc gtccgtactt cgaccacgag ggcgtcgtcg
     1621 cgatgcagcg cagcgatgcg agctgagagg tcatcatcgc tgatgccgtt gcgaatcgct
     1681 cggcgtaacc gacggtcagc ctcagaagtc agcgggtgtt ggtacagcgc ttcaagagcg
     1741 ttggacgtat cgccgtcatg ggcttgcagc gtctcgccta ggcgttgcca gatggcacgt
     1801 cgcacacccc tgagccgtcc cgctaacacg gtcggagccg acagcggacc ctgaaccaac
     1861 tcccgcacca gcacgtcgtg gtcgtcgcgt agcgggcggc ctggttcgtc gggcggagcc
     1921 tcgaacaccc gtagcgcctc gtggccagtc agcagacggg tcgtgccatc cagtgcggcg
     1981 aatccgaagc catccacgcc tgagtgtgtg cgtacgtagc acgccacgcc gtcctccgac
     2041 tcgctagctc ggcgagagcg agtcgcatct agcaagtccg gcaacgcggc gattctgcga
     2101 gccagatcag ggtcattgtc ctccaagttc ttccagtact ggtaggccat gctcgacgca
     2161 tctacctcgc cgccgtcgtc cacgtcgtcg agttggccgt tgtagagatc gactatgacc
     2221 tgcgtctcgc caacggttcc aaagaatcgc tcgtccgacc cgaacgcttc agcgttggca
     2281 gccagccggt ctgcgatgcg ctggcgcaga gcaatcacgt cgtctaccga ctcgtggaag
     2341 aacgagtaca cccgcacctc atgggaggtt tgcccgattc ggtcgatgcg cccggctcgc
     2401 tggataagcc ggatgattgc ccacggcagg tcgtagttca cgacgatgtg agcgtcttgc
     2461 aggttctgac cttcgctgag gacgtccgtc gccaccaaga cgcgcagttc gttggcgggc
     2521 atctcaacgt cctgcccagg taatgcgctc gagaccggcg cgaaacggtg ggccacggcg
     2581 gtcgggttct cggtctcacc agtggccagg ccaacgccat cgacgcccgc cgcctccaac
     2641 tgcgctgcca cgtaggtggc ggtgtctcgg tactcggtga acaccagcac cttctcgttt
     2701 gggtgcgtct tcgcgactag ctcgatcagg gccgccagct tcgagtcgcg atctggcgcc
     2761 caggtgccgt accagtcgag gagtgactgc aactgcgaag tgtcagtatc gaggtccgcc
     2821 cgcagcgcat cgtcaaacag gtcggggcgt acccagcgca gcccagctgg gttggccgac
     2881 agaagggcgg cgtagcgagt tgcaggcgat ccagccgcca gttcgccgtc ggccgcgtcg
     2941 tcgtcggtca cgtcctcgtc gtccatgaag ttggtctcgt tgatggtgcc cgtgggcaca
     3001 ggcaagccgt tgtcgatggc gtaggcgaag aggtcgttgc gggcgatgtg gcgtcgcacc
     3061 gacaggtcga acgagtggcc gcacgacgac aagcgcttgt agaaggtcgt gcgcacgaat
     3121 ccggcaacct gcccgcgacc acgcgtcagg cgatcgacaa agtccttctc gtcatcggtc
     3181 agcgtcaggc ctgaccgcag gtagtcacgc agtcggtatc ggggcagcct gagcgcaccg
     3241 agcacgttg
//
LOCUS       DAORNF010000362         3246 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_22341_length_3246_cov_2.127233, whole genome shotgun sequence.
ACCESSION   DAORNF010000362 DAORNF010000000
VERSION     DAORNF010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3246
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22341_length_3246_cov_2.127233"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            22..600
                     /gene="rplC"
                     /locus_tag="PLV68_13755"
     CDS             22..600
                     /gene="rplC"
                     /locus_tag="PLV68_13755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HRE02363.1"
                     /translation="MFYFLPLQFGVEPARVVQVKTTERDGYTALQVTYGSKDPKKLNK
                     PEAGHFSSAGVAAGRKLIELRLDSVDGYSVGQEIAVDLLSKGQKVDVTAISRGKGNAG
                     VMKRHNFRGQGASHGNHKHHRAPGSIGSCSFPGRVLKGLKMAGHMGHEQVTTLNLEVV
                     EADAERGLLLVKGSVPGPNGGVVIVRNAVKGK"
     gene            605..1498
                     /gene="rplD"
                     /locus_tag="PLV68_13760"
     CDS             605..1498
                     /gene="rplD"
                     /locus_tag="PLV68_13760"
                     /inference="COORDINATES: protein motif:HMM:TIGR03953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HRE02364.1"
                     /translation="MPTVTIHSLSAKGAAGTVELDADMFGVQPNVPVMHQVVTAQLAN
                     RRAGTQSTKTRAEVSGGGKKPFKQKGTGNARQGSTRAPHFSGGGVALGPKPRKYTQRT
                     PKKMIRLALRSALSDRANEGKVVVVDSFGFDKPSTKSAMKALDTLGLTGSVLVVHSRD
                     DDNTILSFRNIPNVQLIEVGELNAYDILCNQTIVFPADLLPTGDPIIDPAGERRAFAA
                     SPAANAEGPYGRGSAAPLESGDAPEGFDIKGNADSMLYHVPGSRYYDQTIAEVWFSTE
                     EAAEAAGFAKPGSKADDEKDS"
     gene            1498..1788
                     /gene="rplW"
                     /locus_tag="PLV68_13765"
     CDS             1498..1788
                     /gene="rplW"
                     /locus_tag="PLV68_13765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HRE02365.1"
                     /translation="MKDPRDIILAPVVSEKSYALIEQGVYTFKVHPDASKPEIHDAVE
                     AIWGVKVTKVNTLNRPGKTKRSRRTNKVGKKPDTKRAIVTLAAGDDIPLFEN"
     gene            1802..2638
                     /gene="rplB"
                     /locus_tag="PLV68_13770"
     CDS             1802..2638
                     /gene="rplB"
                     /locus_tag="PLV68_13770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HRE02366.1"
                     /translation="MAIRIRKPTSAGRRFQTTHDFSEITTSTPEKTLLAKRSGTGGRN
                     NHGRKTARHIGGGHKRQYRIIDFKRTKDGVPATVASIEYDPNRTCRIALLHYADGQKA
                     YILAPKGLTVGAAVRSGQGSDIKPGNAMPMRYMPVGTVIHNVELRPGGGGKIARSAGN
                     SVQLVAKDGNFATLRLPSTEMRRVLIDCRATVGEVGNSEHELVSVGKAGRNRWKGVRP
                     QTRGVAMNPVDHPLGGGEGRTSGGRPAVSPWGKPEGRTRNKKKASQDLIVRRRRTGKG
                     RR"
     gene            2644..2928
                     /gene="rpsS"
                     /locus_tag="PLV68_13775"
     CDS             2644..2928
                     /gene="rpsS"
                     /locus_tag="PLV68_13775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HRE02367.1"
                     /translation="MSRSLKKGPFVDDHLLKKVDTMNAANDKRVIKTWSRRSTIIPDM
                     VGHTIAVHDGRKHVPVYVTESMVGHKLGEFSPTRTFKYHAGQEKNAKGRR"
     gene            2928..>3246
                     /gene="rplV"
                     /locus_tag="PLV68_13780"
     CDS             2928..>3246
                     /gene="rplV"
                     /locus_tag="PLV68_13780"
                     /inference="COORDINATES: protein motif:HMM:TIGR01044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HRE02368.1"
                     /translation="MTGPKLNEKNFVAGSKGGTKAVAKYIRGSASKARIVLNIVRDLP
                     VRQADEVLQFTDKAVAVTIRKVLASAVANAVHNDDQDADELYIKACFADEGPTLRRFA
                     ARAR"
BASE COUNT          633 a         1158 c         1012 g          443 t
ORIGIN      
        1 gcccgtgacg tcgggccgat catgttctac ttcctgccgc tgcaattcgg cgtcgagccg
       61 gctcgcgtcg tccaggtcaa gaccaccgaa cgtgacggct acaccgcctt gcaggtcacc
      121 tacggctcga aggatcccaa gaagctgaac aagcccgagg ccggacactt ctcctcggcg
      181 ggcgtcgcgg ccggtcgcaa gctgatcgag ctacgcctcg acagcgtcga cggctacagc
      241 gtcggccagg agatcgccgt cgacctgctc agcaagggcc agaaggtcga cgtcacagcg
      301 atcagccgtg gtaagggcaa cgccggtgtc atgaagcgcc acaacttccg tggccagggc
      361 gccagccacg gtaaccacaa gcaccaccgt gcccccggtt cgatcggctc ctgcagcttc
      421 ccgggccgcg tgctcaaggg tctgaagatg gccggtcaca tgggccacga gcaggtcacc
      481 acgctcaacc tcgaagtcgt cgaggccgac gccgagcgtg gcttgctgct ggtcaagggc
      541 tcggtccccg gtcccaacgg tggcgtcgtc atcgtccgca acgccgtgaa ggggaagtga
      601 tcccatgccg acagtcacca tccactcgct gagcgccaag ggcgccgccg gcacggtcga
      661 gctcgacgcc gacatgttcg gcgtgcagcc caacgtgccg gtgatgcacc aggtcgtcac
      721 cgcgcagctc gccaaccgcc gcgctggcac ccagagcacc aagacccgcg ccgaggtcag
      781 cggcggcggc aagaagccgt tcaagcagaa gggcaccggc aacgcccgcc agggctcgac
      841 ccgtgcgccg cacttcagcg gcggtggtgt cgccctcggg cccaagccgc gcaagtacac
      901 ccagcgcacc ccgaagaaga tgatccgcct cgccctgcgc tcggcgctgt ccgaccgggc
      961 caacgagggc aaggtcgtcg tcgtcgactc gttcggcttc gacaagccca gcaccaagtc
     1021 ggccatgaag gcgctcgaca cgctcggcct gaccggcagc gtgctggtcg tccacagtcg
     1081 tgacgacgac aacacgatcc tcagcttccg caacatcccc aacgtgcagc tgatcgaggt
     1141 cggcgagctc aacgcgtacg acatcctctg caaccagacg atcgtcttcc cggccgacct
     1201 gctgccgacc ggtgacccga tcatcgatcc cgccggtgag cggagggcct tcgccgccag
     1261 cccggccgcc aacgccgaag gcccgtacgg ccgcggcagc gccgccccgc tggagagcgg
     1321 cgatgcgccc gaaggcttcg acatcaaggg caacgccgac tcgatgctgt accacgtgcc
     1381 tggtagccgc tactacgacc agaccatcgc cgaggtctgg ttctccacgg aagaggccgc
     1441 cgaggctgcg ggcttcgcca agcccggctc caaggccgac gacgagaagg actcgtgatg
     1501 aaagatcccc gcgacatcat cctcgctccg gtcgtgtccg agaagagcta cgccctcatc
     1561 gagcagggcg tctacacctt caaggttcac cccgatgcca gcaagcccga gatccacgac
     1621 gcggtcgagg cgatctgggg cgtcaaggtc accaaggtca acacgctgaa ccggccgggc
     1681 aagaccaagc ggtcgcgccg gaccaacaag gtcggcaaga agcccgacac caagcgggcg
     1741 atcgtcaccc tggcagcggg ggatgacatc ccgctgttcg agaactgagc caggagcagc
     1801 catggcaatt cgcattcgca agcccacgag cgctggtcgt cgtttccaga ccacgcacga
     1861 cttctccgag atcaccacgt cgacgccgga aaagacgctg ctcgccaagc gcagtggcac
     1921 cggtggtcgc aacaaccacg gccgcaagac cgctcgccac atcggcggcg ggcacaagcg
     1981 ccagtaccgc atcatcgact tcaagcgcac caaggacggc gttcccgcca cggtcgcgtc
     2041 gatcgagtac gaccccaacc gcacttgccg catcgccctg ttgcactacg ccgacggcca
     2101 gaaggcgtac atcctggcgc cgaagggcct caccgtcggc gccgcggtgc gcagtggcca
     2161 gggcagcgac atcaagcccg gcaacgcgat gccgatgcgc tacatgcccg tcggtaccgt
     2221 gatccacaac gtcgaactgc gccccggtgg cggcggcaag atcgcccgct cggccggcaa
     2281 ctccgtgcag ctcgtcgcca aggacggcaa cttcgccacc ttgcgtctgc ccagcaccga
     2341 gatgcgccgc gtgctgatcg actgccgggc caccgttggt gaggtcggca actccgaaca
     2401 cgaactggtc agcgtcggca aggccggccg caaccgctgg aagggcgtgc gcccgcaaac
     2461 ccgcggtgtc gcgatgaacc ccgtcgatca ccccctgggt ggtggcgagg gtcgtacctc
     2521 gggtggccgc ccggccgtgt ccccgtgggg caagcccgaa ggccgcacac gtaacaagaa
     2581 gaaggcgtcg caggatctga tcgtccgtcg tcgtcgcacc gggaagggtc ggaggtaatc
     2641 gtcatgtccc gcagcctgaa gaagggtccc ttcgtcgacg accacctgct caagaaggtc
     2701 gacacgatga acgctgccaa cgacaagcgg gtcatcaaga cctggtcgcg gcgcagcacc
     2761 atcatccccg acatggtcgg gcacaccatc gccgtgcacg acggccgcaa gcacgtcccg
     2821 gtgtacgtca ccgagtcgat ggtcggtcac aagctcggtg agttcagccc cacgcgtacc
     2881 ttcaagtacc acgcgggcca ggagaagaac gcaaagggtc gccgctgatg actggtccca
     2941 agctcaacga gaagaacttt gtcgccggca gcaagggcgg caccaaggcc gttgccaagt
     3001 acatccgtgg ctcggccagc aaggcccgca tcgtgttgaa catcgtgcgt gacctgccgg
     3061 tgcgccaggc cgacgaggtg ctccagttca ccgacaaggc cgttgcggtc accatccgca
     3121 aggtgctcgc cagcgccgtc gccaacgcgg tgcacaacga cgaccaggac gccgacgagc
     3181 tgtacatcaa ggcgtgcttc gccgacgagg gtccgaccct gcgccgcttc gccgcccgtg
     3241 cccgcg
//
LOCUS       DAORNF010000363         3246 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_22343_length_3246_cov_1.837355, whole genome shotgun sequence.
ACCESSION   DAORNF010000363 DAORNF010000000
VERSION     DAORNF010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3246
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22343_length_3246_cov_1.837355"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..950)
                     /locus_tag="PLV68_13785"
     CDS             complement(<1..950)
                     /locus_tag="PLV68_13785"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRE02369.1"
                     /translation="MTTPTHSPAARTATLGHSGIEVSRIALGTMMFGSWGNDDEAECH
                     EMTAIALDRGITFFDTADMYDQGRSEEILGRALAGRRSEIVLATKVFNQMGDDPSHCG
                     LSPRWIKQACDDSLRRLGTDYIDLYQMHRPDPDVPIAESLGAMRELVDAGKVRAIGTS
                     VFSAAQLTEAATAGLADDSVRITSEQPPYSILVRAIEADVLPACAANDIGVVVWAPLN
                     GGWLTGKYNTGNAGTESRAARKGDHFDHKDAEMRERKLAMVDQLMTIAADAGLTITEL
                     AMGFVLSNPAITSLLVGPRTPAQLRDLLDAQHVTLSPDILA"
     gene            complement(973..1395)
                     /locus_tag="PLV68_13790"
     CDS             complement(973..1395)
                     /locus_tag="PLV68_13790"
                     /inference="COORDINATES: protein motif:HMM:NF019013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HRE02370.1"
                     /translation="MATAPHPAGNPTESAAAALCRSYLAAFATGNADLVVAHVTHDFV
                     NDHTAALGHSFTGRDEYTRRVPGFLASMPELRYDVEHVTAEGDSVWAAYTMRARVNDR
                     DIALRGAMHFVVRDDLIASRTDYWDSAVFQHQAGLGNE"
     gene            complement(1395..1913)
                     /locus_tag="PLV68_13795"
     CDS             complement(1395..1913)
                     /locus_tag="PLV68_13795"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRE02371.1"
                     /translation="MSYLPSTPAIDPTGHQLGDARLRPTAVRWTHIALPATDIEKTIE
                     WYTTFTPLELLDRREDADGYGAWLGHPDQGDKPFILVLVSFFKDQDKGQQPIMAPFAH
                     IGLEMPSKEAVDAIAEKGKEWGCLAWPPTQMPDPVGYICALTDPDGNMIEFSYDQGVY
                     EKAQEVWGMETA"
     gene            complement(1935..2456)
                     /locus_tag="PLV68_13800"
     CDS             complement(1935..2456)
                     /locus_tag="PLV68_13800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRE02372.1"
                     /translation="MSYPNTLVFVDFPTDDVDKAREFYGQVFNWEVEDRIPQIFSRIV
                     PGQNFKVDDGSQGPTGNLHMGIYNVANARPHPQTEPTAPTNLAPGRGIRAWILVSADD
                     SFERILDTASSLGATELFRNHFWTEFGGGCAAFRDPWGNEIMLWQHLDIERDEQTHQV
                     IGEHVVPDGWTHE"
     gene            complement(2559..>3246)
                     /locus_tag="PLV68_13805"
     CDS             complement(2559..>3246)
                     /locus_tag="PLV68_13805"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRE02373.1"
                     /translation="ISPGASDAVSYLSRTGKRPLVHLHELGVLGPHVVLAHAVHLDDR
                     ECEAALSTRTAIASCPWAYLRLAQGFITHGRHGELYLAGGRLALGCDAENAGDAVDIL
                     RAAALFVGVARDRTSDAYSLTARDGLALATIRGAEALGKADTIGSIEPGKRADLVVHD
                     ARAPQFTPRSTDPVLELVWSTDGRSVNDVVVDGRIVIRDKRCVTVDIDALRDEAQARR
                     DHFLSLRNGG"
BASE COUNT          473 a         1015 c         1175 g          583 t
ORIGIN      
        1 gcgaggatgt cggggctgag ggtgacgtgt tgcgcgtcga gcaaatcgcg gagttgggct
       61 ggggtgcggg ggccgaccag cagcgaggtg atcgctgggt tggagagcac gaaccccatt
      121 gccagttcgg tgatcgtgag cccggcgtcg gcggcgatcg tcatcaactg atcgaccatg
      181 gccagcttgc gctcgcgcat ctcggcgtct ttgtggtcga agtggtcacc cttacgcgca
      241 gcccgtgatt cggtgcccgc gttgcctgtg ttgtacttgc cggtgagcca tccaccattg
      301 agcggcgccc acaccacgac gccgatgtcg ttggccgcgc acgccggcag cacgtcggcc
      361 tcgatggccc gcaccaggat cgagtacggc ggctgctcag aggtgattcg caccgagtcg
      421 tcggccagtc cagccgttgc ggcttcggtc aactgcgccg cgctgaacac cgaggtgccg
      481 atcgcgcgca ccttgcccgc gtcgaccagc tcgcgcatcg cgcccagcga ctcggcgatc
      541 ggcacgtccg gatcgggacg gtgcatctgg tacaggtcga tgtagtcggt gcccagccgg
      601 cgcaggctgt cgtcgcaggc ctgcttgatc cagcgcgggg acaagccgca gtgggagggg
      661 tcgtcaccca tctggttgaa gaccttggtg gcgagcacga tctcgctgcg ccggccggcc
      721 agggcgcggc cgaggatctc ctccgagcgg ccctggtcgt acatgtcggc tgtgtcgaag
      781 aaggtgatgc cgcgatcgag agcgatcgcg gtcatctcgt ggcactcggc ttcgtcgtcg
      841 ttgccccacg agccgaacat catcgtgccg agcgcgatgc ggctgacctc gatgccgctg
      901 tggccgagcg ttgccgtgcg agcggcgggg gagtgtgtag gagtcgtcat tcggttctgt
      961 ctgtggtgag ggctactcgt tgccgaggcc ggcttggtgc tggaacaccg cgctgtccca
     1021 gtagtcggtg cggctggcga tcaggtcgtc gcgcacgacg aagtgcatcg cgccacggag
     1081 ggcgatgtcg cggtcgttga cgcgggcgcg catcgtgtac gcggcccaca ccgaatctcc
     1141 ctcggcggtc acgtgctcga cgtcgtagcg caactccggc atcgatgcca gaaaccctgg
     1201 gacgcggcgg gtgtactcgt cgcggccggt aaagctgtgg ccgagcgcgg cggtgtggtc
     1261 gttgacgaag tcgtgggtca cgtgggcgac gaccagatcg gcgttgccgg ttgcgaacgc
     1321 ggcgagatac gaccggcaca gcgccgcggc ggcgctctca gtcgggttgc cagcggggtg
     1381 aggcgccgtg gccatcaggc cgtctccatc ccccacacct cttgtgcctt ctcgtacacg
     1441 ccctgatcgt aggagaactc gatcatgttg ccgtcggggt cggtgagcgc gcagatgtaa
     1501 cccaccgggt cgggcatctg tgtgggcggc caggcgaggc agccccactc cttgcccttc
     1561 tcggcgatcg cgtcgaccgc ttccttgctg ggcatctcca ggccgatgtg cgcgaacggc
     1621 gccatgatcg gctgctggcc tttgtcctgg tccttgaaga agctgaccag caccaggatg
     1681 aacggcttgt cgccctgatc ggggtggccg agccatgccc cgtagccgtc ggcgtcctcg
     1741 cggcggtcga gcagttccag cggggtgaag gtggtgtacc actcgatcgt cttctcgatg
     1801 tcggtggcgg gcagcgcgat gtgagtccag cgcaccgcgg tgggccgcag ccgggcgtcg
     1861 ccgagttggt gacccgtggg gtcgatcgcg ggggtggaag gcaagtagct catcgacgtt
     1921 tccttactcg tgggtcactc gtgggtccag ccgtcgggca cgacgtgctc gccgatgacc
     1981 tggtgggtct gttcgtcacg ctcgatgtcg aggtgctgcc acagcatgat ttcgttgccc
     2041 cacgggtcgc ggaacgctgc acagccaccg ccgaactcgg tccagaagtg gttgcggaac
     2101 agctcggtgg caccgaggct gctcgccgtg tcgaggatgc gctcgaagga gtcgtcggcg
     2161 ctgaccagga tccaggcccg gatgccgcgg ccgggagcga ggttggtcgg tgcggtgggc
     2221 tcggtctgcg ggtgcggcct ggcgttggcg acgttgtaga tgcccatgtg caggttgccc
     2281 gtcggcccct gcgagccgtc gtccaccttg aagttctggc cgggaacgat gcggctgaag
     2341 atctggggga tgcggtcttc gacctcccag ttgaagacct ggccgtagaa ctcgcgggcc
     2401 ttgtcgacat cgtcggtcgg gaagtcgaca aagacgaggg tgttcggata gctcatggtt
     2461 ggacagtttg tcagaccaaa gagggttcct caactcggag cgacattctt ggactgatgg
     2521 tctgaccatc tatgtggact acgctgagtg tcgtgcgatc acccgccgtt gcgcagcgac
     2581 aagaagtggt cgcgccgcgc ctgtgcttcg tcgcgcaggg cgtcgatgtc gacggtgacg
     2641 cagcgcttgt cgcggatcac gatgcggccg tcgaccacga cgtcgttgac cgaccgcccg
     2701 tcggtagacc acaccaactc cagcacgggg tcggtgctgc gcggcgtgaa ctgcggggcg
     2761 cgggcgtcgt gcacgaccag gtcggcgcgc ttgccgggct cgatcgaacc gatcgtgtcc
     2821 gccttgccca gtgcttcggc gccgcggatg gtcgccagcg cgaggccgtc gcgggcggtc
     2881 aaggagtacg catcgctcgt tcggtcgcgc gcgacgccga cgaacagcgc cgcggcgcgc
     2941 aggatgtcga ccgcgtcgcc ggcgttctcg gcgtcgcagc ccagcgccag gcgaccgccc
     3001 gccagataca gctcgccgtg gcggccgtgg gtgatgaagc cctgggccag gcgcaagtac
     3061 gcccaggggc acgacgcgat cgcagtgcgc gtcgagagcg ccgcctcgca ttcccgatcg
     3121 tccagatgca ccgcatgggc caacacgacg tgcggaccga gcacgcccag ttcgtgcagg
     3181 tgcaccaatg ggcgcttgcc ggtgcggctc aggtagctga ccgcgtcgct ggcgccgggc
     3241 gagatg
//
LOCUS       DAORNF010000364         3231 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_22475_length_3231_cov_1.845088, whole genome shotgun sequence.
ACCESSION   DAORNF010000364 DAORNF010000000
VERSION     DAORNF010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3231
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22475_length_3231_cov_1.845088"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="PLV68_13810"
     CDS             complement(<1..340)
                     /locus_tag="PLV68_13810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HRE02374.1"
                     /translation="MAASRDWFEKDFYKVLGVSETASAKDITKAYRKLARDNHPDTHP
                     GDEAAENRFKEVSAAYDVVGDEAKRAEYDEVRRQGPMGPFAGGFTNGGGSPGGFSFNV
                     GADGLGDLLGG"
     gene            complement(359..>784)
                     /locus_tag="PLV68_13815"
     CDS             complement(359..>784)
                     /locus_tag="PLV68_13815"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HRE02375.1"
                     /translation="EPLSETEQLAREVAEFKDMALRIQADFENYRKRVVSQQADDIDR
                     ATGRFVEALLPVLDACEAGFAHGGAGVEAIWSQLMGILTKQGLEAFDLVGQPFDPAKA
                     EAVIHEPGDGGEATVTEVLRTGYAWKGKTLRAAMVKVRG"
     assembly_gap    785..908
                     /estimated_length=124
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(928..2745)
                     /gene="dnaK"
                     /locus_tag="PLV68_13820"
     CDS             complement(928..2745)
                     /gene="dnaK"
                     /locus_tag="PLV68_13820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HRE02376.1"
                     /translation="MAKAVGIDLGTTNSVVAFLEAGEPVVIPNSEGARTTPSVVAFSK
                     SGEVLVGEVAKRQAITNPDRTFRSIKREMGTNWKSPDIDGKKYTPQEISARTLMKLKR
                     DAEAYTGDTVTQAVITVPAYFDDAQRTATKEAGQIAGLEVLRIINEPTAAALAYGLDK
                     GTEDETVLVFDLGGGTFDVSVLEIGDGVFEVKSTHGDTSLGGYDWDQRVIDWLVQQFK
                     AAHGVDLAQDRMAGQRLKEAAEKAKIELSQVQQTQINLPFITATADGPLHLDESLSRS
                     KFQEMTADLIERCRIPFEQALKDAGVSKGQLHHVILVGGSTRMPAVADLVQNLTGKEP
                     NRSVNPDEVVAVGAAIQAGVLKGDVKDVLLLDVTPLSLGIETKRGVMTKLIERNTEVF
                     TTAEDMQPSVEIHVLQGEREMASYNKTLGKFQLVDLPPAPRGVPQIEVTFDIDANGIV
                     HVSAKDRATSKEQSMTISGQSTLSKNDIDQMVKDAEAHAEEDRQRREEAEVRNNADSA
                     IYQAEKMLRDQGESAAAADKEAVEGPLATLKSAVSGNDNGAIKEATDALSMALQAFGQ
                     KLYEAASRDTNATGSSASGQDSASDDGDIVDAEIVDDEK"
     gene            2989..3189
                     /locus_tag="PLV68_13825"
     CDS             2989..3189
                     /locus_tag="PLV68_13825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02377.1"
                     /translation="MRAALSSAVQSPVVTSPVVKLGLFGALLVALVLGGAAVGAAIGP
                     DGHPNPGHSPATTTTVTGHDHG"
BASE COUNT          440 a         1024 c         1016 g          627 t
ORIGIN      
        1 tgccgccgag caggtcgccc agcccgtcgg ccccgacgtt gaagctgaac ccgccgggcg
       61 atccgccgcc gttggtgaac ccaccggcaa agggacccat cggaccctgg cggcggacct
      121 cgtcgtactc ggcccgcttg gcctcgtcac cgacgacgtc gtaggcggcg gagacctcct
      181 tgaagcggtt ctccgccgcc tcgtcaccgg ggtgggtgtc ggggtggttg tcgcgcgcga
      241 gcttgcggta cgccttggtg atgtctttgg cactggccgt ctcgctgacg cccagcacct
      301 tgtagaagtc cttctcgaac cagtcacgcg acgctgccat ggcaatcctt ccccgggctc
      361 agccccgcac cttgaccatc gccgcgcgca aggtcttgcc cttccacgcg tagccggtgc
      421 gcaacacctc ggtcaccgtc gcctcgccgc cgtcgcccgg ttcgtggatg acggcttcgg
      481 ccttggccgg atcgaacggc tgaccgacca ggtcgaaggc ttcgagaccc tgcttggtga
      541 ggatgcccat gagttgactc cagatcgcct cgacgcctgc tccgccgtga gcgaatccgg
      601 cctcgcacgc gtcgagcacc ggcagcaacg cttcgacgaa acggccggtc gcccgatcga
      661 tgtcgtcggc ctgttgggac acgacgcgct tgcggtagtt ctcgaagtcg gcctggatgc
      721 gcagcgccat gtccttgaac tcggccacct cgcgggcgag ctgctcggtc tcggacaacg
      781 gctcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      901 nnnnnnnnga ttcgtcatcg gcgaaggtca cttctcgtcg tcgacgatct cggcatcgac
      961 gatgtcaccg tcgtcgctgg ccgagtcctg accgctggcc gagctaccgg ttgcgttggt
     1021 gtcacgcgag gccgcctcgt acagcttctg accgaaggcc tgcagtgcca tcgacagcgc
     1081 atcggtggct tccttgatcg caccgttgtc gttgccactg acggccgact tcaacgtggc
     1141 gaggggaccc tcgaccgctt ccttgtcggc agccgccgcg ctctcgccct gatcacgcag
     1201 catcttctcg gcctggtaga tcgccgagtc ggcgttgttg cgtacttcgg cctcttcgcg
     1261 gcgctgacgg tcttcctcgg cgtgggcctc ggcgtctttg accatctggt caatgtcgtt
     1321 cttgctcagc gtgctctggc cgctgatcgt catcgactgc tccttgctgg tggcccggtc
     1381 cttggcggac acgtgcacga tgccgttggc gtcgatgtcg aacgtgacct cgatctgcgg
     1441 cacgccacgg ggcgctggtg gcaggtcgac gagctggaac ttgcccagcg tcttgttgta
     1501 gctggccatc tctcgctcgc cctgcaggac gtggatctcg acgctgggct gcatgtcttc
     1561 cgcggtggtg aacacctcgg tgttgcgctc gatcagcttg gtcatcacgc cacgcttggt
     1621 ctcgatgccg agcgacagcg gcgtcacgtc gagcaacaac acgtccttga cgtcgccctt
     1681 caacacgccg gcctggatgg ccgcgccgac ggccaccacc tcgtcggggt tgaccgagcg
     1741 gttcggctcc ttgccggtga ggttctggac caggtcggcc acggccggca tgcgggtgga
     1801 gccgccaacc aggatgacgt ggtggagctg acccttgctg acgcccgcgt ccttcaacgc
     1861 ctgctcgaac ggaatgcggc agcgctcgat gagatcggcg gtcatctcct ggaacttgct
     1921 gcggctgagc gactcgtcga ggtgcagcgg gccatcggcc gtcgcggtga tgaagggcag
     1981 gttgatctgg gtctgctgca cctggctgag ttcgatcttg gccttctcgg ccgcctcctt
     2041 gaggcgctgg ccagccatgc ggtcttgcgc cagatcgacg ccgtgggcgg ccttgaactg
     2101 ctgcaccagc cagtcgatga cgcgctggtc ccagtcgtac ccacccaggc tggtgtcgcc
     2161 gtgggtgctc ttcacttcga acacgccgtc gccgatctcg agcacggaga cgtcgaaggt
     2221 gccaccgccg aggtcgaaca cgagcaccgt ctcgtcctcg gtgcccttgt cgaggccgta
     2281 ggccagggcg gccgcggtgg gctcgttgat gatgcgcaac acctcgaggc cggcgatctg
     2341 gccggcttcc ttggtggcgg tgcgctgggc gtcgtcgaag taggccggca cggtgatgac
     2401 ggcctgggtg acggtgtcac cggtgtaggc ctcggcgtca cgcttgagct tcatcagcgt
     2461 gcgcgcgctg atctcttgcg gcgtgtactt cttgccgtcg atgtcagggc tcttccagtt
     2521 ggtgcccatc tcgcgcttga tgctgcggaa ggtgcggtct gggttggtga ttgcctgacg
     2581 cttggcgacc tcgccgacga gcacctcgcc ggacttgctg aacgcgacga cgctgggggt
     2641 ggtgcgggcg ccttccgagt tggggatgac gaccggctcg ccggcttcga ggaaggcgac
     2701 gacgctgttg gtggtaccga ggtcgatacc gactgccttg gccatgtctg aatgtccttt
     2761 gttctggtga agggtcttgg tgagcaagac ggatgggaga tcgggttccg cccgatgttg
     2821 agcggacctg tagcaaggtt atccacaact tcagggaagc gacacgagaa cctcaaacat
     2881 cttgagtcgt catcactcaa gttttccggg cgctcaagtg tgttggctct caaggttgtc
     2941 gggatcgagg aggatgacct tgataccccc atggggtata ctgaccccat gcgcgcagcc
     3001 ctgtcctccg cagtccagtc ccctgtcgtc acctcccctg tcgtcaagct cggtctgttc
     3061 ggcgcgttgc tcgtggcgtt ggtgctcggc ggtgcggccg tcggagcggc gatcggcccc
     3121 gacggccacc ccaaccccgg ccactcaccc gccaccacca cgacggtgac aggacacgac
     3181 catggctgac acgacgacgc ccgacgcgac aacgctgagc agtcctggcg a
//
LOCUS       DAORNF010000365         3228 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_22498_length_3228_cov_1.820044, whole genome shotgun sequence.
ACCESSION   DAORNF010000365 DAORNF010000000
VERSION     DAORNF010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3228
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22498_length_3228_cov_1.820044"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(142..660)
                     /locus_tag="PLV68_13830"
     CDS             complement(142..660)
                     /locus_tag="PLV68_13830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02378.1"
                     /translation="MAQNVIRQLIDSGLNFTEDSRAKAEKLVKELVKAGEIRRKDAEA
                     TVQTLVTRGKESSDQLLAFIQAEVAKQIARFTERIDDVEDRLEDFAEKLGIKAAAKQA
                     AATPAAATSPVKKAAPAKKAPAKKAPAKKAAPATATAAKAPAKKVAAKQVAAKQAPAK
                     RVAARKAAASAS"
     gene            complement(718..>1102)
                     /locus_tag="PLV68_13835"
     CDS             complement(718..>1102)
                     /locus_tag="PLV68_13835"
                     /inference="COORDINATES: protein motif:HMM:NF024639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HRE02379.1"
                     /translation="VRAGARLVATNDDATFPTPDGLIPGGGAILAGIATAAGVAPEVA
                     GKPYAPMAALVRAVTGHSDLTDAVMVGDRPDTDGRMAVALGCRYAQVRSGVTAPGVTV
                     RPAPWLDVADLAAVADHLLVRTGPM"
     assembly_gap    1103..1180
                     /estimated_length=78
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1181..1484)
                     /locus_tag="PLV68_13840"
     CDS             complement(<1181..1484)
                     /locus_tag="PLV68_13840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02380.1"
                     /translation="MTNNSYRRVAEHEAALAAIGIPAVGDVLSSAMAAALMVEPHERA
                     LVCGGPGVVEALTERGVRCTLADHDQPEHDQTDRDQLNHDQAGHDQASDDEAGHDQA"
     assembly_gap    1492..1584
                     /estimated_length=93
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1585..2825
                     /locus_tag="PLV68_13845"
     CDS             <1585..2825
                     /locus_tag="PLV68_13845"
                     /inference="COORDINATES: protein motif:HMM:NF018004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HRE02381.1"
                     /translation="AGNVHRGSLAWQPVRVPGFEPFRALRYAPDRDLDRVSAPPYDVI
                     DEHDVARLLSVDPDNIVAVDFPLEANGPGRYDAAAATLHDWVERGVLVADPTPSFTLY
                     RMQFRDETGTERETVGVLGALEVVDEGAGGVLPHERTTPKAKTDRLDLTIATEANLSP
                     VWGLSLAAGLTDVLREPGEAMGRCTDEEGVVHIVERVDDPARVAAIRAAVSSAAVVIA
                     DGHHRYAISRTYRDRQRGTTGRDDLESERTLTYVGELVADQLSVAAIHRLVRGVSADE
                     LIAGLDACFDAAPAGTVTPAFARTVVERGALCVVHPDGSGTWLTPRPGAFDGVRDLDS
                     ARLEHALAALPAAEISYQHGVAHIVDAVTSGRADAGVLIRPVSIAEIRRTADEGALMP
                     PKSTFFSPKLRTGLVIRPLH"
     gene            complement(2853..>3228)
                     /locus_tag="PLV68_13850"
     CDS             complement(2853..>3228)
                     /locus_tag="PLV68_13850"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376843.1"
                     /note="catalyzes the formation of glycerone phosphate and
                     D-glyceraldehyde 3-phosphate from D-fructose
                     1,6-bisphosphate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HRE02382.1"
                     /translation="GVTIEADIIKQKLPENNGGYNALSGYGKTHARVYDTLTTDHPID
                     LARYQVANCYMGRIPLINSGGESKGAGDLAAAVKTAVVNKRAGGLGLISGRKAFQRPM
                     AEGVELLNAIQDVYLDASITIA"
BASE COUNT          431 a         1099 c         1029 g          498 t
ORIGIN      
        1 tcaggatgtt cttcgcctcg acggcctgcg tacggctggt caccaaaccg cggcgcagca
       61 tctcggcatc gagccggcgc cgacgagaca cgaatgagtc gcccgacgcg cagcagacag
      121 gattggtgac gacgcgccgg ctcagctggc cgacgcggcg gccttcctgg cggcgacgcg
      181 cttggccggt gcctgcttgg cggcgacctg cttggcagcg accttcttgg cgggggcctt
      241 ggccgcggtg gccgtggcgg gagccgcctt cttggccgga gccttcttgg ccggagcctt
      301 cttcgccggt gcagccttct tgaccgggct ggtcgcagca gccggtgtcg cggcggcctg
      361 cttggccgca gccttgatgc cgagcttctc ggcaaagtct tcgaggcgat cctcgacgtc
      421 gtcgatccgc tccgtgaagc gggcgatctg cttggccact tccgcctgga tgaaggcgag
      481 caactgatcg gacgactcct tgccgcgggt gaccagggtc tgcaccgtgg cctcggcgtc
      541 cttgcggcga atctcacccg ccttgacgag ctccttgacc agcttctcgg ccttggcccg
      601 cgagtcttcg gtgaagttca atcccgagtc gatgagttga cggatcacgt tctgtgccat
      661 ggggtcaagc gtaccccgcc tgagcagggc acaagacgcg tcgtgcctgg ctagcggtca
      721 catcggcccg gtccggacga gcagatgatc ggccactgcg gccaggtcgg cgacgtcgag
      781 ccacggcgcc gggcgcaccg tcacaccggg cgctgtgacc cccgaacgca cctgggcgta
      841 gcggcagccg agcgcgaccg ccatgcgacc gtcggtgtcg ggacgatcac cgaccatgac
      901 cgcgtcggtc agatcagagt ggccggtcac cgcccgcacc aacgcggcca tcggcgcata
      961 cggcttgccc gccacctccg gggccacccc ggccgcggtg gcgatcccgg ccaggatcgc
     1021 gccgccaccg gggatcagac cgtcgggcgt ggggaacgtc gcgtcgtcgt tggtggcgac
     1081 cagacgggcg ccggcgcgca cgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cggcctggtc atggccggcc
     1201 tcgtcatcgc tggcctggtc atggccggcc tggtcatggt tgagctgatc gcgatctgtc
     1261 tggtcatgct cgggctggtc gtgatctgcc agcgtgcagc gcacgccacg ctcggtcagg
     1321 gcctcgacca cgcccgggcc gccgcacacc agtgcccgtt cgtgtggctc gaccattagc
     1381 gccgccgcca tcgccgagct gagcacgtcc ccgacggcgg ggataccgat cgccgcgagc
     1441 gcggcttcgt gctcggcgac gcggcgatac gagttgttgg tcacgaacag cnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnngggccg ggaatgtcca tcgcggcagt ctcgcatggc
     1621 aacctgtacg ggtgcctggt ttcgaaccct tccgcgccct gcgctacgca cccgaccgtg
     1681 acctcgaccg ggtgtccgcg ccgccgtacg acgtgatcga cgaacacgac gtcgcccggc
     1741 tgctgagcgt cgaccccgac aacatcgtcg cggtcgactt ccccctcgaa gccaacggcc
     1801 ctggccgata cgacgcggcc gcggcgaccc tgcacgactg ggtcgagcgc ggcgtgctgg
     1861 tcgccgatcc cactccgagc ttcacgctct accgcatgca gttccgcgac gagaccggca
     1921 ccgaacgcga gaccgtcggc gtgctcggcg cgctcgaagt cgtcgacgaa ggtgccggtg
     1981 gcgtgctgcc ccacgagcgg accacaccca aggccaagac cgaccgcctc gatctcacca
     2041 tcgccaccga ggccaacctg tcgccggtgt gggggctgtc gctggctgcc ggcctcaccg
     2101 acgtgcttcg cgagccgggc gaggcgatgg gccgctgcac cgacgaggag ggcgtggtcc
     2161 acatcgtcga gcgggtcgac gaccccgccc gcgtcgcagc gatccgcgct gcggtgtcgt
     2221 cggccgcggt cgtcatcgcc gacggtcacc accgctacgc catctcgcgc acctaccgcg
     2281 atcgccaacg tggcaccacc ggccgcgatg atctggaatc cgagcgcacc ctcacctacg
     2341 tgggcgaact ggtcgccgac cagctcagcg tggccgcgat ccaccggctc gtgcgcggtg
     2401 tgtccgccga cgagttgatc gccgggctcg acgcttgctt cgacgccgca cccgctggca
     2461 cggtgacacc ggccttcgcc cgcaccgtgg tcgagcgggg cgcactgtgc gtcgtgcatc
     2521 ccgacggcag cggtacctgg ctcacgccac gtcccggcgc gttcgacggc gtgcgcgatc
     2581 tcgacagcgc gcggctcgaa cacgccctcg cagcgttgcc cgcggccgag atcagctacc
     2641 agcacggcgt ggcccacatc gtcgatgcgg tcacctcggg gcgcgccgac gcgggcgtgt
     2701 tgatccgccc ggtcagcatt gccgagattc gccgcaccgc cgacgaaggc gcgctgatgc
     2761 cccccaaaag cacgttcttt tcgcccaagc tgcgcaccgg cctggtgatc cgccccctcc
     2821 actgagcgtt gggcggatca gccggggcga tgtcaggcga tcgtgattga cgcgtcgagg
     2881 tagacgtctt gaatcgcgtt gagcagttcg acgccttcag ccatcggccg ctggaacgcc
     2941 ttgcgacccg agatcaggcc cagcccaccg gcacgcttgt tgacgacggc ggtcttgacc
     3001 gccgcggcca agtctcccgc gcccttgctc tcgcccccgc tgttgatcag ggggatgcgg
     3061 cccatgtagc agttggcgac ctggtaccga gcgagatcga tcgggtgatc ggtggtgagg
     3121 gtgtcgtaga cccgcgcatg cgtcttgccg tagcccgaca acgcgttgta gccaccgttg
     3181 ttctcgggca gcttctgctt gatgatgtcg gcctcgatcg tcaccccg
//
LOCUS       DAORNF010000366         3217 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_22581_length_3217_cov_2.008223, whole genome shotgun sequence.
ACCESSION   DAORNF010000366 DAORNF010000000
VERSION     DAORNF010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3217
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22581_length_3217_cov_2.008223"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(142..987)
                     /locus_tag="PLV68_13855"
     CDS             complement(142..987)
                     /locus_tag="PLV68_13855"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRE02383.1"
                     /translation="MVETLTNGVPTLDLTGQNALITGAASGIGAATARRFAALGATLH
                     LADIDDARGAALASELGATYHHLVVADPGAWAALDVATQVDIIFLNAGVSTRPPDAEP
                     NDVPMGEVTDAAYRRIMSINVDGVVFGARAALPAMVARGGGHIVITASMAGLGPIPFD
                     PIYGLTKHAMVGFAKSLGVTYGPMGVCTSAICPGFADTNIISGELKDMLGQVGVPVMS
                     ADRVADTVVTALAAAEPGSLWAVWGDLPIARYEPNPVFPEMEQGIGSMLDLPEAMAAD
                     EAASA"
     gene            complement(1009..2226)
                     /locus_tag="PLV68_13860"
     CDS             complement(1009..2226)
                     /locus_tag="PLV68_13860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRE02384.1"
                     /translation="MPGAFLHPFASPSRESFVRIVRGHGASVWDADGNELIDGMASLW
                     YCAIGHGRREMADAVGAQLSTLAAYSCFDPFTNGPADELAEKLVEIGPIPGARVFFTD
                     SGSESVDTAMKLARLAHVQAGHPERTLIISRTRGYHGTNYGGTSAQGIAPNRVGYGPL
                     VPEVVQVDSDDIEGLATLMKDRSSEVAAVITEPVQGAGGVFPPERDYLDDLRRLCDHH
                     GAFLIFDEVITGFGRLGSWFAAQHYGVTPDFVTFAKAVTSGYQPLGGVYVGPAPLAAL
                     EADPAFILRHGYTYSGHAAVCAAGLKNLEIIERESLIERATHVGQRLSDGLRSLAEDG
                     TIDHVRGVGAVWATGLRADQNAAAIRDHMLAAGAIVRALPDSNVFCPPLVITDEQIDR
                     LVDIFAAAVIATT"
     gene            2269..>3217
                     /locus_tag="PLV68_13865"
     CDS             2269..>3217
                     /locus_tag="PLV68_13865"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRE02385.1"
                     /translation="MCTPIWRAPGSSAPLLMRVVQLGHRHTHPLKADMNHPHPSPNAR
                     DPRPRRRRPKNAHPGPRSQNSAPALADSLTDVATIVDADATFESLGVPQQVVAALAAQ
                     GIDRPFPIQTATLGDSLAGRDVLGRGKTGSGKTIAFAVPVVARLAASRMRPQPGRPRG
                     LVLVPTRELANQVAETIAPLAAALRLRHTVIFGGVGQTPQVNALRAGVDIVIACPGRL
                     EDLIGQGHCRLDQVEVTVLDEADHMADLGFLPAVRRLLDATPAGIQRLLFSATLDNGI
                     DVLVQRYLSDPVVRSVDPAVAPVAAMEHHVFAVTGADKLV"
BASE COUNT          493 a         1105 c         1105 g          514 t
ORIGIN      
        1 cggaggcggt cgtagaacgg atacggatcg gcgcgaaacg ccggatcgaa ggggttgaac
       61 agaatctcgt ccatcatgtc tcttctatca cgtctcttct gtcacgtcgc tctgtcacgt
      121 cgctctgtca cgtctcgtct gtcacgcaga cgcggcctcg tcggcggcca tcgcttcggg
      181 cagatccagc atcgaaccga tcccctgctc catctccggg aacacggggt tgggctcgta
      241 gcgggcgatc ggcaagtcgc cccacaccgc ccacagcgaa ccgggctcgg ccgccgcgag
      301 cgcggtgacc accgtgtcgg cgacgcgatc ggccgacatg acgggcacgc cgacctggcc
      361 gagcatgtcc ttcaactcgc cggagatgat gttggtgtcg gcaaagccgg ggcagatcgc
      421 gctggtgcac acgcccatcg ggccgtaggt cacgccgagc gacttggcga agccgaccat
      481 ggcgtgtttg gtcaggccgt agatcgggtc gaacgggatg ggaccgagcc ccgccatcga
      541 ggcggtgatc acaatgtggc cgccgccgcg ggccaccatg gcaggcagtg cggcgcgggc
      601 tccgaacacc acgccatcga cgttgatgct catgatccgc cgatacgccg cgtcggtgac
      661 ctcgcccatc ggcacgtcgt tcggctccgc gtcgggcggg cgggtgctga caccagcgtt
      721 gaggaagatg atgtcaacct gggtcgcgac gtcgagcgcg gcccaggcgc cggggtcggc
      781 gacgacgagg tggtgatagg tcgcgccgag ttcggacgcc aacgcggcac ctctggcatc
      841 gtcgatgtcg gccagatgca gggttgctcc cagcgcggcg aaccgccgcg ccgtcgccgc
      901 accgatgccg ctcgctgctc ccgtgatcag cgcgttctgg cccgtgagat cgagtgtcgg
      961 aacgccgttc gtgagtgttt ccaccatgag cggtatgtaa ccacgccgtc aggtcgtggc
     1021 tatgaccgca gcggcgaaga tgtcgaccag gcggtcgatc tgctcgtcgg tgatgaccag
     1081 cggggggcag aagacattgc tgtcgggcag ggcacgcacg atcgccccgg ccgcgagcat
     1141 gtggtcacgg attgccgctg cgttctgatc ggcgcgcagg cctgtggccc acactgcccc
     1201 gacgccgcgg acgtgatcga tcgtgccgtc ctcggccagg gagcgtaggc cgtcgctgag
     1261 gcgctgaccg acgtgggtgg cccgttcgat cagcgactcg cgctcgatga tttccaggtt
     1321 cttcaggccc gcggcgcaca cggcggcatg gccgctgtag gtgtagccgt ggcgcaggat
     1381 gaatgcagga tcggcttcga gcgcggccag gggagcgggg ccgacgtaga cgccgccgag
     1441 gggctggtag ccggacgtga ccgccttggc aaaggtgacg aagtccggcg tcaccccgta
     1501 gtgctgggcg gcgaaccacg agccgagccg cccgaagccg gtgatcacct cgtcgaagat
     1561 caagaacgcg ccgtgatgat cgcacaggcg gcgcagatcg tcgaggtagt cgcgctcggg
     1621 cgggaacacg ccgcccgccc cctgcaccgg ctcggtgatc accgccgcga cctcgctgct
     1681 gcggtccttc atcaacgtcg ccaagccctc gatgtcgtcg ctgtcgacct ggaccacctc
     1741 gggcaccagc ggcccgtagc cgacgcggtt gggggcgatg ccctgcgcgc tggtgccgcc
     1801 gtagttcgtg ccgtggtaac cgcgggtgcg gctgatgatc agcgtccgct ccgggtgacc
     1861 ggcctgcacg tgggccagcc gggccaactt catcgcggtg tcgacggact cgctaccgct
     1921 gtcggtgaag aacacccgtg ccccggggat ggggccgatc tcgaccagct tctcggccag
     1981 ctcgtcggcc gggccgttgg tgaagggatc gaagcacgag taggccgcaa gggtggagag
     2041 ctgggcgccg acggcgtcgg ccatctcgcg ccgcccgtga ccgatcgcgc agtaccacag
     2101 gctggccatg ccgtcgatca actcgttgcc gtcggcgtcc cacacggacg cgccgtgacc
     2161 gcgcacgatg cgcacgaacg actcgcgaga tgggctggca aaggggtgaa ggaaggcacc
     2221 gggcatgact gccacggtat tcgctcggcg ccggtaacgt agccacttgt gtgcacgccg
     2281 atctggcgtg cgcccgggtc gagtgccccg ctgttgatgc gggtcgtgca gcttggccat
     2341 cgccatacgc accccctgaa agcagacatg aaccaccccc accccagccc gaacgcgcgc
     2401 gacccgcgcc cgcgtcgccg tcgccccaag aacgcacacc ccggcccgcg cagccagaac
     2461 agcgcaccag cgctcgccga ttcgctcacg gacgtcgcga cgatcgtcga tgccgacgcc
     2521 acgttcgagt cgctcggcgt acctcagcag gtcgtcgccg cgctcgccgc gcagggcatc
     2581 gaccgcccct ttccgatcca gaccgcgacc ttgggcgaca gcttggccgg gcgcgacgtg
     2641 ctcggccgcg gcaagaccgg cagcggcaag acgatcgcct tcgccgttcc ggtggtagcc
     2701 cgtctggccg cgtcgcgcat gcgtccgcag ccgggtcgcc ctcgtggcct cgtactggtg
     2761 ccgacgcgcg agcttgccaa ccaggtcgcc gagacgatcg ccccgttggc cgccgcgctg
     2821 cggttgcgtc acaccgtgat cttcggtggg gtcggtcaga ccccgcaggt caatgccttg
     2881 cgcgccggag tcgacatcgt catcgcctgc cctggccgcc tggaggactt gatcggtcag
     2941 gggcattgcc gcctcgatca ggtcgaagtc accgtgttgg acgaggccga ccacatggcc
     3001 gacctgggct ttctccctgc tgtgcggcgt ttgctcgacg ccaccccggc gggtattcag
     3061 cggttgctgt tctcggccac gctcgacaac ggcatcgacg tgctggtgca gcgctacctc
     3121 agcgatcccg tcgtccgctc ggtcgaccca gcggtggcgc cggtcgcggc aatggagcat
     3181 catgtcttcg ccgtcaccgg cgcagacaag ctggtcg
//
LOCUS       DAORNF010000367         3211 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_22639_length_3211_cov_1.676489, whole genome shotgun sequence.
ACCESSION   DAORNF010000367 DAORNF010000000
VERSION     DAORNF010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3211
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22639_length_3211_cov_1.676489"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="PLV68_13870"
     CDS             complement(<1..644)
                     /locus_tag="PLV68_13870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02386.1"
                     /translation="MSSSDPSVIGVSLRARETRRAEHTIGVLGSPWRATVSSGGSIRP
                     WDGSGDVQWFVAADDRWHIPEQEPTVRQRRLDGTPVIETRVKVPSGDAIQRVWTVPDG
                     GVLTVIEIENESPMAFAVAFDRVDLLSARPPADVPIQGIDLPAGAVVFPVGHHTTLRF
                     GLAHDPGATWAPERLPAAMQVARGWTAVLDRAGRIVVPDAATIETVSYLRSELA"
     gene            complement(668..>1723)
                     /locus_tag="PLV68_13875"
     CDS             complement(668..>1723)
                     /locus_tag="PLV68_13875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02387.1"
                     /translation="PRLIAQVIEVSAQVSTNRVYRLVLADGSSVIAKVSSYGSFYLFR
                     EDHDRILRTRELLQGTRWANLLADVFVRRGNSRDADGYRDRSRHGEVFTYHTKELWTC
                     FYQDVPVRQTLPRILSNDQIHDLGEEMALLHRECADVARRIPLSSKSIKSDAIHLLEL
                     LSDKHSSLKFQFDREQKDYLRSQCHEFLEQLDEFGYDDWLRIPVLIDWNLGNFSVDLA
                     RHHHDTRPLRIRLFSRWDYDWFRIEPRLLDFYFLSRVSSATGDRTVFSYSPHTLLEPR
                     FKRFLSAYHRIFPLHEREVLFLREVYRFFILNYVVREGDNFFQPEYWHRLQQEVVSLC
                     LPAAQRLDLRPLLDVLE"
     assembly_gap    1724..1733
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1870..>2226
                     /locus_tag="PLV68_13880"
     CDS             1870..>2226
                     /locus_tag="PLV68_13880"
                     /inference="COORDINATES: protein motif:HMM:NF014085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02388.1"
                     /translation="MDAAVLVPVKDFRQAKARLAPVVSPGDRVRLARWTADGVIAAAA
                     PLPVFVACDDDEVAAWAHDRGATVLWRPEVGLNAAVQHGIARLAADGIEHVIVVHSDL
                     ATPRPLADLVLSGGAVL"
     assembly_gap    2227..2366
                     /estimated_length=140
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2488..>3211
                     /locus_tag="PLV68_13885"
     CDS             2488..>3211
                     /locus_tag="PLV68_13885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HRE02389.1"
                     /translation="MVANEPGQPAPSPGAWSIDLDVPRVALAIGAHPDDIEFGAGATL
                     AKWALAGCVVHHLVLTDGSKGTWNPDADIAALVHDRQVEQRAAARIVNGDRDAGTVTF
                     LGQIDGELTHSVALRADVVRCIREIRPDVVLGHDPWKRYRLHPDHRNAGLVTCDAIVA
                     ARDPHFHRDQGLTHHRPATLLLWEADAADHVEDVTGFVDAKLHALEAHESQFESTMKA
                     TDEAQIEAFRVRIRERLSGLGAA"
BASE COUNT          517 a         1033 c         1055 g          456 t
ORIGIN      
        1 aaggccaact cactgcgcag gtatgacacc gtctcgatcg tggccgcgtc gggcaccacg
       61 atgcgcccgg cacggtcgag cactgcggtc catcctcgcg ccacctgcat cgcggcgggc
      121 agccgttcgg gagcccacgt ggcacccgga tcgtgggcca ggccgaatcg cagcgtggtg
      181 tgatggccga ccgggaacac caccgcgcca gccggtaggt cgatgccttg gatcggcacg
      241 tcggccgggg ggcgggcaga gagaaggtcg acccggtcga acgcgaccgc gaacgccatc
      301 ggcgactcgt tctcgatctc gatcaccgtc aggacgccac cgtcgggcac ggtccacacc
      361 cgctggatcg catcgccgct gggcaccttg acccgcgtct cgatcaccgg cgtgccgtcg
      421 aggcggcgct ggcgcacggt cggctcctgc tcggggatgt gccaccggtc gtcggcggcg
      481 acgaaccact gcacatcacc cgatccgtcc cacggccgga tcgagccacc ggaggacacc
      541 gtggcccgcc aaggactacc gagcaccccg atggtgtgtt cggcgcggcg ggtctcgcgg
      601 gcccgcaggg agacaccgat gacggatggg tcgctgctcg acatggtccc gtacggtacc
      661 ggcgggttca ctcgagcacg tcgagcaacg ggcgcaggtc gagccgttgc gccgcaggca
      721 ggcacagcga gacgacctct tgctgtaacc ggtgccaata ctcgggctgg aagaagttgt
      781 caccttcgcg cacgacgtag ttgaggatga agaagcgata cacctcgcgc aagaacagca
      841 cctcgcgctc gtgcaacgga aagatgcggt ggtaggccga caggaaccgc ttgaaccgcg
      901 gctccaacag ggtgtgcggc gagtacgaga acacggtgcg gtcaccggtg gcgctggaca
      961 cccgggagag gaagtagaag tcgagcagcc gcggctcgat gcggaaccag tcgtagtccc
     1021 accggctgaa caggcggatg cgcaacggcc gcgtgtcgtg gtggtgacgg gcgaggtcga
     1081 ccgagaagtt gccgaggttc cagtcgatca agacggggat gcgcagccag tcgtcgtagc
     1141 cgaactcgtc gagttgttcg aggaactcgt ggcactggct acgcaggtaa tccttctgct
     1201 cacggtcgaa ctggaacttc agcgacgaat gcttgtcgga cagcagttcc aacaggtgga
     1261 tcgcgtcgga cttgatcgac ttggacgaca gcgggatccg gcgggcgacg tcggcgcact
     1321 cgcgatgcag cagcgccatc tcttcaccca ggtcgtggat ctggtcgttg gacaggatgc
     1381 gcggcagggt ctggcgtacc ggcacgtcct ggtagaagca ggtccacaac tccttggtgt
     1441 ggtacgtgaa cacctcgccg tggcgtgacc ggtcgcggta accgtcggcg tcacggctgt
     1501 tgccacggcg gacgaacacg tcggcgagca ggttggccca gcgggtgccc tggagcagtt
     1561 cgcgcgtgcg caggatgcgg tcgtgatctt cgcggaacag gtagaacgac ccgtacgacg
     1621 acaccttggc gatcacgctg gaaccgtcgg ccagcaccag gcggtagacg cggttggtcg
     1681 acacctgcgc actcacctcg atgacctgcg cgatcaggcg cggnnnnnnn nnnacccaac
     1741 cggcgccggc cagcaggata tcgtgcgcga tgcggaacag cagccggtcg agcgccgagc
     1801 gtcggccgtc ggcgacatgg cgccagccta ggtgcatcgc gctgacgtcg gctcgttagt
     1861 gtgagccggg tggacgccgc ggtgctcgtt ccggtcaagg acttccgcca ggcgaaggca
     1921 cggctggcac ctgtggtgtc gcccggcgac cgcgtccgtc tcgcccgctg gaccgccgac
     1981 ggtgtgatcg ccgcggcggc ccccctgccg gtgttcgtcg cctgtgacga cgacgaggtt
     2041 gccgcatggg cgcacgaccg cggtgccacc gtgctgtggc gccccgaggt cggtctcaac
     2101 gccgcggtcc aacacggcat cgcccgtctg gccgccgacg gcatcgagca cgtcatcgtc
     2161 gtgcacagcg acctggccac gccgcggccg ttggccgacc tggtgctgag cggcggggcg
     2221 gtgttgnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnncccg ctcctcgcgc tcgacatcga cacgccagcc
     2401 gatctcgatc acccgctggt gcgccccttg gtcgccgccg cgctcggcca ctgaggacgc
     2461 gacgatgacc cacaaggaga caccctgatg gtcgccaacg aacccggaca accggcaccc
     2521 tcacccggtg cgtggagcat cgacctcgac gtgccccgcg tggcgttggc gatcggcgct
     2581 caccccgacg acatcgagtt cggtgcggga gccaccttgg ccaagtgggc cctggccggc
     2641 tgcgtcgtgc atcacctcgt actgaccgat gggtcgaagg gtacgtggaa ccccgatgcc
     2701 gacatcgctg cactggtgca cgaccgtcag gtcgaacagc gtgccgcggc ccgaatcgtc
     2761 aacggcgacc gcgacgcagg gaccgtcacg tttctcggcc agatcgacgg tgaactgacc
     2821 cacagcgtcg cgctgcgggc cgacgtggtg cgctgcatcc gcgagatacg ccccgacgtc
     2881 gtgctcggtc acgacccgtg gaagcgctac cgcctgcacc ctgaccaccg caacgccgga
     2941 ctggtcacct gtgacgccat cgtcgccgca cgcgacccgc acttccaccg cgatcagggt
     3001 ctcacccacc accgtccggc cacgctgttg ctgtgggagg ccgacgccgc cgatcacgtc
     3061 gaggacgtca ccggtttcgt cgatgcgaag ctgcacgcgc tcgaggcaca cgagtcgcag
     3121 ttcgagagca cgatgaaggc cacggacgag gcacagatcg aggcgttccg cgtgcggatc
     3181 cgcgagcgcc tgtcggggct cggcgcggcg c
//
LOCUS       DAORNF010000368         3210 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_22649_length_3210_cov_1.956577, whole genome shotgun sequence.
ACCESSION   DAORNF010000368 DAORNF010000000
VERSION     DAORNF010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3210
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22649_length_3210_cov_1.956577"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1028)
                     /locus_tag="PLV68_13890"
     CDS             complement(<1..1028)
                     /locus_tag="PLV68_13890"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HRE02390.1"
                     /translation="MLRFGTDGVRGVALTELTIDDVTALGRAAVEVLGGGTWLVGRDT
                     RESGPELEAALVRGLCAAGASVQRLGVVPTPAVAFHAAARGLPAAMITASHNPYHDNG
                     VKFFGAGGLKLSDAVERSIEAAAVAAVDAAAGSASALSADAVCSTDAEAGMHTDGSGA
                     GAAYVAHITGLFPAGALTGMSVVLDCANGAMSGVAPAAVAALGADVQVIHAEPDGRNI
                     NDRCGATHTASLTAAVVAAGADAGLAFDGDGDRVIAVDATGAVVDGDRMIALGALQLH
                     AEGALTADTVVVTVMTNLGFHRAMQEAGIHVIATAVGDRYVLEALDAGDYSIGGEQSG
                     HVIYRHLAT"
     gene            complement(1039..1434)
                     /gene="rpsI"
                     /locus_tag="PLV68_13895"
     CDS             complement(1039..1434)
                     /gene="rpsI"
                     /locus_tag="PLV68_13895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HRE02391.1"
                     /translation="MATKPLTQTTGRRKESVARVRLRPGTGVVTVNGRTFENYFPTAM
                     ARMIVTEPLRLTETEEAYDVDCSIFGGGISGQAGALRMGIARSLVEIDPEQRPTLKKA
                     GLLTRDPRKKESKKYGLKKARKAPQYTKR"
     gene            complement(1473..1922)
                     /gene="rplM"
                     /locus_tag="PLV68_13900"
     CDS             complement(1473..1922)
                     /gene="rplM"
                     /locus_tag="PLV68_13900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HRE02392.1"
                     /translation="MMRTYSPKASEITRAWHVIDAEGMVLGRVCTEAARLLRGKHKPM
                     FAPHLDTGDHVIIVNADKVVLTSNKADNKFVYRYSGYPGGLKTETYANKLGRKPDQAV
                     RDSVKGMLPKGPLGRQMLTKLKVYAGPNHPHAAQLPVAHALPGAKAR"
     gene            complement(2092..>3210)
                     /locus_tag="PLV68_13905"
     CDS             complement(2092..>3210)
                     /locus_tag="PLV68_13905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02393.1"
                     /translation="LLSAMYVFKRLSGVAAVQTLSRLNRTYVTPAGQVKATTMVLDFV
                     NDAAEIQAAFQPYFRDAHIETETDPNIVNDIAAKLDQANLYTSAEIEQAAAAWVASNL
                     RKSGGHNALAAAVQPAQQRFWAAHAHAEAEGDKTELAKLDLFRRDVATFVRVYDFMSQ
                     IIDYGAELEKRANFYRLLERLLRASRPDEQIDLGDVTLRTVAQVNRGATDIKLGVDDD
                     AGLKGITGAGSGARREPKMVALQEVIDRLNDLFADAALTAGQKQTFVEGLLHTLLADA
                     TLVAQAQANTKKQFLESPRLTDGVLDAVADNQAAHDAMADTFSTDTATRNDIIRLVGA
                     ALYEWVVAERSGAGSSDATTWVIEADEEVPELAERSGG"
BASE COUNT          492 a         1081 c         1092 g          545 t
ORIGIN      
        1 gtggccaaat ggcggtagat gacgtgcccg ctctgctcac cgccaatcga atagtcaccg
       61 gcgtcgagcg cttcgagcac gtagcgatca ccgacggccg tggcaatcac atggatgccc
      121 gcttcttgca tggcgcgatg gaacccgagg ttcgtcatca cggtcaccac caccgtgtcg
      181 gccgtgaggg caccctcggc gtggagttgc agcgcaccga gggcgatcat ccggtcgccg
      241 tcgaccaccg cgccggtcgc atcgaccgcg atcacccggt cgccgtcgcc gtcgaaggcc
      301 agccccgcgt cggcaccggc ggccaccacg gctgcggtga gcgaggcggt gtgcgtcgct
      361 ccgcagcggt cgttgatgtt gcgcccatcg ggctcggcgt ggatcacctg cacatcggca
      421 cccagcgcag cgaccgccgc cggagcaaca ccggacatcg ccccgttggc gcaatcgagc
      481 accaccgaca ttcccgtgag cgcgccggcg ggaaacaaac ccgtgatgtg cgcgacgtac
      541 gcggcccccg ccccgctccc gtcggtgtgc atgccagcct cagcgtcagt actgcagaca
      601 gcgtccgcgc tgagtgctga cgcagaacca gccgcagcat cgacagccgc gaccgcggcc
      661 gcttcgatcg accgctcgac cgcgtcgctg agcttgaggc cgcctgcgcc gaagaacttg
      721 acgccgttgt cgtggtacgg gttgtgcgag gcggtgatca tcgccgcggg caggccacgg
      781 gccgcggcat gaaaggcaac ggcgggcgtg ggcaccaccc ccaaccgctg cacgctcgcc
      841 ccggcagcgc agaggccgcg caccagcgcg gcctccagct cgggtcccga ctcgcgggta
      901 tctcgtccaa ccagccacgt ccccccgccg agcacctcga cggcggctcg gcccagggcg
      961 gtgacgtcgt cgatggtcag ttcggtgagg gcgacgccgc ggacaccatc ggtgccgaaa
     1021 cgcagcacga gccgcggatc agcgcttggt gtactgcggc gccttacggg ccttcttgag
     1081 gccgtacttc ttgctctcct tcttacgcgg gtcacgcgtg agcaagccgg ccttcttcag
     1141 cgtgggacgc tgctcgggat cgatctcgac cagcgagcga gcgatgccca tgcgcagcgc
     1201 accggcctgg ccgctgatgc cgccaccgaa gatcgagcag tcgacgtcgt aagcctcttc
     1261 ggtctcggtg agacgcagcg gctcggtgac gatcatccgt gccatcgccg tggggaagta
     1321 gttctcgaac gtgcgaccgt tgacggtgac gacgccggtg cccggacgca ggcgaacgcg
     1381 agcgaccgac tccttgcggc ggccggtggt ctgagtgagc ggcttggttg ccatgtgtat
     1441 cagttcttcc tacagcagtc gttcggcggt gatcagcggg ccttggcgcc ggggagggcg
     1501 tgggcgacgg gcagctgcgc agcgtgaggg tggttcgggc ccgcgtacac cttgagcttg
     1561 gtcaacatct ggcgaccgag ggggcccttg ggcagcatgc ccttgacgct gtcgcgcacg
     1621 gcctggtcgg gcttgcggcc gagcttgttg gcgtaggtct cggtcttgag gccacccggg
     1681 tagccgctgt agcggtacac gaacttgttg tcggccttgt tggacgtgag cacgaccttg
     1741 tcggcgttga cgatgatgac gtgatcgccc gtgtcgaggt ggggggcgaa catcggcttg
     1801 tgcttgccgc gcagcaaccg cgcggcctcg gtgcacacac ggccgagcac catgccctcg
     1861 gcgtcgatga cgtgccacgc gcgagtgatc tcgctggcct tgggtgaata ggtgcgcatc
     1921 atggcagtca aatcctcgaa actcttcgcg atctcgtagt cggcacagtc gcgaggggcc
     1981 gggtgcgcgg tcgcaccgac cgcctggcgc cgggacccga caaggatagg ggcgtgccac
     2041 gacgttcgac aatgcgccag ccgcgaaaac tggcccggct cgccgatcgc cctaaccacc
     2101 ggagcgttca gcaagctccg gtacctcctc gtccgcctcg atcacccagg tggtggcgtc
     2161 ggacgaaccg gccccagaac gctcggccac gacccattcg tacagcgccg cgccgaccag
     2221 gcggatgatg tcgttccgtg tcgcggtgtc ggtggagaac gtgtcggcca tcgcgtcgtg
     2281 ggccgcctgg ttgtcggcca cggcgtcgag cacgccatcg gtgaggcgag gcgattcgag
     2341 gaactgcttc ttggtgttgg cctgcgcctg ggcgaccaat gtcgcgtcgg ccagcaaggt
     2401 gtgcagcaga ccctcgacga aggtctgctt ctgcccagcg gtcaatgcag cgtccgcgaa
     2461 cagatcgttg agccgatcga tcacctcctg cagcgccacc atcttcggct cgcgccgggc
     2521 tcccgacccg gcaccggtga tgcccttcag cccggcatcg tcgtcgacgc cgagtttaat
     2581 gtcggtggcg ccgcggttca cctgggcgac ggttcgcagg gtcacgtcgc ccaggtcgat
     2641 ctgttcgtcc ggtctcgacg cgcgcagcag ccgttcgagc agccggtaga agttggcccg
     2701 cttctccaac tcggcgccgt agtcgatgat ctgcgacatg aagtcgtaga cgcgcacgaa
     2761 ggtggcaacg tcacggcgga acaggtcgag cttggccagt tcggtcttgt cgccctccgc
     2821 ctcggcgtgc gcgtgggccg cccagaaccg ttgctgggcc ggctgcaccg ctgcggcgag
     2881 ggcgttgtgg ccaccgctct tgcgcagatt gctcgccacc cacgcggccg cagcctgttc
     2941 gatctcggcc gaggtgtaga ggttcgcctg gtcgagcttg gcggcgatgt cgttgacgat
     3001 gttcgggtcg gtctcggtct cgatgtgcgc gtcgcggaag tacggctgga acgccgcctg
     3061 gatctcggct gcgtcgttga cgaagtcgag caccatcgtc gtcgccttca cctgaccagc
     3121 cggagtgacg taggtgcggt tcaaccgcga cagcgtctgc accgcggcca cacccgacag
     3181 acgcttgaac acgtacatcg ccgacagcaa
//
LOCUS       DAORNF010000369         3196 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_22802_length_3196_cov_1.641515, whole genome shotgun sequence.
ACCESSION   DAORNF010000369 DAORNF010000000
VERSION     DAORNF010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3196
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22802_length_3196_cov_1.641515"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    272..391
                     /estimated_length=120
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<392..772)
                     /locus_tag="PLV68_13910"
     CDS             complement(<392..772)
                     /locus_tag="PLV68_13910"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRE02394.1"
                     /translation="MPDRIETERLVLRRWRVEDMPLLRAAITASIEHLRPWMPWVQHE
                     PVSDADRVALIESWEQRWRDGIDFTMGVFLRDPDGVAPGAGGEISIGGSGLHRRGEPD
                     SLEIGYWIHSDHIRHGYAREAAAAL"
     assembly_gap    793..835
                     /estimated_length=43
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            989..1246
                     /locus_tag="PLV68_13915"
     CDS             989..1246
                     /locus_tag="PLV68_13915"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRE02395.1"
                     /translation="MPDDLDDPSDASTPLVFDAVGLSMRGLTLLRDFSASVAAGETLA
                     VMGPSGAGKTTLLRAIAGLVAPCAGSIRRAGRVAMVFRTRG"
     gene            1246..1698
                     /locus_tag="PLV68_13920"
     CDS             1246..1698
                     /locus_tag="PLV68_13920"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRE02396.1"
                     /translation="MPWRTAIDNVAIVLSRQGRTQARAEARAGAMAWLDRVGLAEAAH
                     VYPGALSGGMRQRVAIARALAARAPIVLVDEPFSNLDAATASVLRDDLTGYLRAERRA
                     VVWVTHDAAEAAAVGNRTLTMHGPPDGHWAIGARPERPRTPIPTDRSS"
     gene            1698..2852
                     /locus_tag="PLV68_13925"
     CDS             1698..2852
                     /locus_tag="PLV68_13925"
                     /inference="COORDINATES: protein motif:HMM:NF014658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02397.1"
                     /translation="MKRTIGIVTLAMALLAAACGSDGDSASPSTSQTTAASSTTTPNT
                     DAATTTVASSTSTADATTTTAVTTTTAAPTTTAAPEPLDALRIYHPETLAFAAPFALL
                     DPDGPLAAVANDVSTEVWATPDVLRSVLVNGQSDVTAVPTYVGANLANRGVDVQLAAV
                     MVWGLLWVIGPDGTASSWDSLRGQTVMVPFQNDMPDLVFRYLAAANGLTPGDDFTIEY
                     YAQPPEAVARLVSGNGTWAVLPEHVATIALAQAGQNGHALSRVFDLQQEWASATGGSP
                     RIPQAGILVPTSLAADRPDVIGAVLDELERAVAAINAAEPDTLARLSEASGLPEPIIA
                     QVIPRLNLDVVPAAEARAELERFYTELATLSPDIIGGKLPDPSFYLADPR"
     gene            2849..>3196
                     /locus_tag="PLV68_13930"
     CDS             2849..>3196
                     /locus_tag="PLV68_13930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02398.1"
                     /translation="MSDRGRGLAGAAIGVVVIVAVWALAARGQPQLILPSPAETWTAL
                     VGLASDGDVASALAITLRRAVVGVAIGLVIGVVWGAANGWSRWTAAITQPALAALMAI
                     PPVVLVALGMVWFG"
BASE COUNT          424 a         1068 c         1055 g          486 t
ORIGIN      
        1 gcggcaaggt tgaccccggc gtcgctgacc gcagacgcga tcgaagcgag tgagcccggc
       61 gtgttggcca cctcgatgac gatgtcgacc gacatgaatt cccctctcca tgtacccccc
      121 accccaagtg ggttgccccg cattgtgtca tccccgccaa ccccggcgat cctcgaccgc
      181 gcgacgtgga gcgtgtgtca ggtgctccag gcgtcgcgcg tgatcgacca cacgaggtgg
      241 acgccggtct cggcgggtgc ttgcgcggcg annnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngagtgccgc agcggcctcg cgggcgtatc
      421 cgtggcggat gtggtcggag tgaatccagt agccgatctc caacgagtcg ggctcgccgc
      481 ggcggtggag cccgctaccc ccgatggaga tctcgccgcc ggcaccgggc gcgacaccgt
      541 cggggtcgcg caggaacaca cccatcgtga aatcgatgcc gtcgcgccag cgctgctccc
      601 acgactcgat caacgcgacg cggtcggcgt cgctgaccgg ctcgtgctgc acccacggca
      661 tccacgggcg cagatgttcg atgctggccg tgatcgcggc gcgcagcaac ggcatgtcct
      721 ccacccgcca ccgccgcagc accaaccgtt ccgtctcgat ccgatctggc actggttcgg
      781 cgtccatggc ccnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnggagg
      841 ggattgggga ctttggtccc tgatccgagc tggttaggca accctaatgt caatccacag
      901 gagctgttgc caccgggcgg ctccgtgagt cgacaagctt ggaggaggct gcatcgtgac
      961 gattcgtcct cggcgcggga gcgtccgctt gccggacgat ctggacgacc cgtccgatgc
     1021 atccactccg ctggtgttcg atgcggtcgg attgtcgatg cgcgggctga cgttgctgcg
     1081 cgacttctcg gcgtcggttg cggcgggcga gacgctcgcc gtgatgggtc cctccggggc
     1141 gggaaagacc acgctgctgc gcgcgatcgc cggcctggtc gcgccctgcg ctggcagcat
     1201 tcgccgcgcc gggcgggtgg cgatggtatt caggacccgc ggctgatgcc gtggcggacc
     1261 gcgatcgaca atgtcgccat cgttctgtcc cgccagggtc gcacccaggc tcgtgccgag
     1321 gcgcgcgctg gggcgatggc gtggttggac cgggtcggtc tcgccgaggc cgcgcacgtc
     1381 tatccgggtg cactgtcagg cggcatgcgc cagcgggtcg cgatcgctcg cgccctcgcc
     1441 gcccgggctc cgatcgtgct ggtcgacgaa ccgttctcga acctcgacgc ggcgaccgcc
     1501 tcggtgctgc gcgacgacct gaccggttac ctgcgcgccg agcgtcgcgc tgtggtctgg
     1561 gtgactcacg acgccgccga agcggccgcg gtcggcaatc gcaccctgac gatgcacggt
     1621 ccacccgacg gacactgggc gatcggcgcg cgcccggaac gacctcgtac cccaatcccg
     1681 acagacagga gttcttgatg aaacgaacga tcggcatcgt cacgctcgcg atggcattgc
     1741 tcgccgccgc gtgcggctcc gacggcgaca gcgcgtcgcc ctcgacctcg caaaccaccg
     1801 cggcgtcatc cacgacgacc ccgaacaccg atgctgcgac caccaccgtc gcgagttcga
     1861 cgtcgaccgc ggacgcgacg acgactaccg cggtcaccac gacgacggcc gcgccgacga
     1921 ccacggccgc tcccgagccg ctcgacgcgt tgcgcatcta ccaccccgag acgctcgcct
     1981 tcgcggcgcc gttcgccttg ctcgacccgg acggtccgct cgctgccgtg gccaacgacg
     2041 tctccaccga ggtgtgggcc acgcccgacg tgctccgctc ggtgttggtc aacggccaga
     2101 gtgacgtcac cgccgtgccg acctacgtcg gtgccaacct ggccaaccgc ggtgtcgacg
     2161 tgcaactggc cgcggtcatg gtgtggggtc tgttgtgggt gatcggaccg gacggcacgg
     2221 cgtcgtcgtg ggacagcctg cgcggtcaga cggtgatggt gccgttccag aacgacatgc
     2281 ccgacctggt gttccgctat ctcgctgcgg ccaacgggct gacgccgggc gacgacttca
     2341 cgatcgagta ctacgcccag ccgcccgagg cggtcgcccg gctggtgagc ggcaacggca
     2401 cctgggcggt gttgcccgaa cacgtcgcca cgatcgcgct tgcccaggcg ggtcagaacg
     2461 ggcatgcact cagccgcgtg ttcgacctgc aacaggagtg ggcgagtgcg accggcggtt
     2521 cgccacgcat tccccaggcg ggcatcttgg tgccgacctc actcgccgct gaccggcccg
     2581 acgtgatcgg cgcggtgctc gacgagttgg agcgggccgt cgctgcgatc aacgctgccg
     2641 agcccgacac cctcgcccgt ctgtccgaag cgtcgggtct gcccgagccg atcatcgccc
     2701 aggtgatccc tcggctgaac ctcgacgtgg tgcccgccgc agaggcccgc gcggaactgg
     2761 aacggttcta caccgaactg gccacgttgt cgcccgacat catcggcggg aagctgcccg
     2821 accccagctt ctacctcgcc gacccgcggt gagcgaccgc ggccgcggcc tggccggcgc
     2881 ggccatcggc gtcgtggtga tcgtcgcggt gtgggcgttg gcagcgcggg gtcagccgca
     2941 gttgatcctg ccctcgccag cggagacctg gacggcgctg gtcggcctcg ccagtgacgg
     3001 cgacgtcgcg tctgcactgg cgatcacact gcggcgggcc gtggtcggcg tggcgatcgg
     3061 gttggtgatc ggtgtcgtgt ggggtgcggc caacgggtgg tcgcggtgga cggccgcgat
     3121 cacccagccc gcgctggccg cactgatggc gattccgccc gtggtgctgg tggcgctggg
     3181 catggtgtgg ttcggg
//
LOCUS       DAORNF010000370         3188 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_22889_length_3188_cov_1.903607, whole genome shotgun sequence.
ACCESSION   DAORNF010000370 DAORNF010000000
VERSION     DAORNF010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3188
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22889_length_3188_cov_1.903607"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..875)
                     /gene="hrcA"
                     /locus_tag="PLV68_13935"
     CDS             complement(<1..875)
                     /gene="hrcA"
                     /locus_tag="PLV68_13935"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HRE02399.1"
                     /translation="MLDDRKTAILRAVVQEYIATAQPVGSSHVIGAPGVQVSSATVRN
                     DMAALEHDGYLVQPHTSAGRIPTDKGYRFFVDHLTPSGRLDEAAAQQVGDFFNRAHGR
                     LEQLLASTSDLLARLTHHTAVVVGASPPAAAVVRSVQIVALSPRHATVVAVLSNGHVH
                     NHTIELDDDTTDARVAAATAALAQAAVGSALGSIITPVTGDDVVDALSAQALAVLVPD
                     DDRSPVFVGGAASVAKSFAAVDVVRSVLATLEQEYVVVSLVRDVLDRGLSVAIGAEHG
                     LEPLSSCSVIVKSVAV"
     gene            complement(957..>1266)
                     /locus_tag="PLV68_13940"
     CDS             complement(957..>1266)
                     /locus_tag="PLV68_13940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441667.1"
                     /note="back-translocating Elongation Factor EF4; binds to
                     the ribosome on the universally-conserved alpha-sarcin
                     loop; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HRE02400.1"
                     /translation="FSTIVHRDKAFDYGRRMTEKLKELIPRQMFDVPIQAAIGGKIIA
                     RETVKAKRKDVLAKCYGGDITRKRKLLEKQKEGKKKMKSIGRVEIPGDVFISALKLDN
                     "
     assembly_gap    1267..1445
                     /estimated_length=179
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1446..>1697)
                     /locus_tag="PLV68_13945"
     CDS             complement(<1446..>1697)
                     /locus_tag="PLV68_13945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441667.1"
                     /note="back-translocating Elongation Factor EF4; binds to
                     the ribosome on the universally-conserved alpha-sarcin
                     loop; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HRE02401.1"
                     /translation="KERLEREFNLALIATAPSVEYLVHRTDGVVQKVDNPADLPPVQK
                     IAHIEEPFFRVSIITPKEYTGTLMDLCQTRRGEMLKLEYL"
     assembly_gap    1698..1795
                     /estimated_length=98
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1796..>2226)
                     /locus_tag="PLV68_13950"
     CDS             complement(<1796..>2226)
                     /locus_tag="PLV68_13950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HRE02402.1"
                     /translation="AVVERIPAPTGDAGAPLQALIFDSQYDQYRGVVSSIRVVNGVMQ
                     ANSRLKFMNAATTHEAIEIGVRSPVMTPMAQLGPGEVGYLIAGIKDVGQARSGETVTT
                     FHNSATEALAGYRDPKPMVFCGLYPIDGDDFENLRESIEKLR"
     assembly_gap    2227..2273
                     /estimated_length=47
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2274..2755)
                     /locus_tag="PLV68_13955"
     CDS             complement(<2274..2755)
                     /locus_tag="PLV68_13955"
                     /inference="COORDINATES: protein motif:HMM:NF012239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRE02403.1"
                     /translation="MDAPALDIGLDRIRNFSIIAHIDHGKSTLSDRILEMTHAVEVRD
                     MRAQYLDTMDLERERGITIKAQNVRVRWRDHVLHLIDTPGHVDFGYEVSRSLAACEGV
                     VLVVDAAQGIEAQTLANCYLALEHDLEIVAVLNKIDLPAADPDRYAGEIETVLGIPAD
                     "
     gene            complement(2833..>3188)
                     /locus_tag="PLV68_13960"
     CDS             complement(2833..>3188)
                     /locus_tag="PLV68_13960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02404.1"
                     /translation="SERNTDDLDDERAEYLEKNCGITSSSDDDGSSDDGDSPSISVKG
                     GAEGLRQLLQLIAAGSGGTVTDEQIDCAVKALDGKLTDADIQGITTGQISQEGSVAFA
                     LALVGCNIDFGALGS"
BASE COUNT          423 a          929 c          993 g          519 t
ORIGIN      
        1 acggctaccg acttgacgat gaccgaacac gaactgagcg gttcgagccc gtgctcggcg
       61 ccgatcgcca cactgagacc acggtcgagc acgtcgcgca ccagcgacac caccacgtat
      121 tcctgttcga gcgtggccag cacgctgcgc accacgtcga ccgcagcgaa cgacttggcc
      181 accgacgcgg caccgccgac gaacaccggc gaacggtcgt cgtcgggaac cagcacggcg
      241 agcgcctggg cgctgagggc gtcgaccacg tcgtcgccgg tgaccggggt gatgatcgag
      301 ccgagcgccg agccgacagc agcctgcgcc agcgcggcgg tggcagcagc gacgcgggcg
      361 tcggtggtgt cgtcgtcgag ttcgatggtg tggttgtgga cgtggccgtt ggacagcacc
      421 gccaccaccg tggcgtggcg cggcgagagc gcgacgatct gcaccgaacg gaccactgcg
      481 gccgccggcg gcgacgcccc gaccaccacg gcggtgtggt gggtgaggcg ggcgagcaga
      541 tcgctggtgc tggccagcaa ctgttcgagc cgcccgtggg cccggttgaa gaagtcgccg
      601 acctgttggg ctgccgcctc gtcgaggcgc ccggacgggg tgagatggtc gacgaagaag
      661 cggtacccct tgtcggtcgg gatgcgcccg gcgctggtgt ggggctggac gagatagccg
      721 tcgtgttcga gcgcggccat gtcgttgcgc accgtggccg acgacacctg cacgcccggc
      781 gccccgatga cgtgcgagga cccgacgggc tgagccgttg cgatgtactc ctggacgacg
      841 gcccggagaa ttgcggtctt gcgatcgtcg agcatggccg tactgagaat accgtcgagg
      901 gattgccgcc gggtggctca gcgtccgtgt ctcgctggtg cctcgcccgt atccggtcag
      961 ttgtcgagct tgagggcaga gatgaacacg tcaccgggga tctcgacccg accgatcgac
     1021 ttcatcttct tcttgccctc cttctgcttc tccaacagct tgcgcttgcg ggtgatgtcg
     1081 ccgccgtagc acttggcgag cacatccttg cgcttggcct tgaccgtctc gcgggcgatg
     1141 atcttgccgc cgatcgcggc ctggatcggc acgtcgaaca tctgccgcgg gatcagttcc
     1201 ttcagcttct cggtcatccg tcgcccgtag tcgaacgcct tgtcgcgatg cacgatcgtg
     1261 ctgaacnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnncaggt actccagctt gagcatctcg ccgcggcggg tctggcacag gtccatcaac
     1501 gtgccggtgt actccttggg cgtgatgatg ctgacccgga agaacggctc ttcgatgtga
     1561 gcgatcttct gcaccggtgg cagatcggcc gggttgtcga ccttttgcac cacaccgtcg
     1621 gtgcgatgga ccaagtactc cacgctgggg gcggtggcga tgagggcgag gttgaactcg
     1681 cgctcgagcc gctctttnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncggag
     1801 cttctcgatg ctctcgcgca ggttctcgaa gtcgtcaccg tcgatgggat agaggccgca
     1861 gaacaccatc ggcttggggt cgcggtagcc cgccagcgct tcggtggccg agttgtggaa
     1921 cgtggtgacc gtctcaccgc tgcgggcctg gccgacgtct ttgatgcccg cgatcaggta
     1981 gccgacctcg ccgggaccga gttgggccat cggcgtcatc accgggctgc ggacgccgat
     2041 ctcgatcgct tcgtgcgtcg tggcggcgtt catgaacttg agccgcgagt tggcctgcat
     2101 caccccgttg accacacgga tgctgctgac cacgccgcgg tactggtcgt actggctgtc
     2161 gaagatcagc gcctgcaacg gtgcgccggc gtcgccggtg ggggcgggga tgcgctcgac
     2221 caccgcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntcgtcgg
     2281 cggggatgcc gagcacggtc tcgatctcgc ccgcgtaccg atcggggtcg gccgcgggca
     2341 ggtcgatctt gttgagcacg gcgacgatct ccagatcgtg ctccaacgcc aggtagcagt
     2401 tggccagggt ctgggcctcg atgccctggg ccgcgtcgac caccaacacc acgccttcgc
     2461 aggccgccag gctgcggctg acctcgtagc cgaagtcgac gtgaccgggg gtgtcgatca
     2521 ggtgcagcac gtggtcgcgc cagcgcacgc gcacgttctg ggccttgatc gtgatgcccc
     2581 gctcgcgctc caagtccatc gtgtcgaggt actgggcgcg catgtcgcgc acttcgaccg
     2641 cgtgggtcat ctcgaggatg cggtcggaca gggtcgactt gccgtggtcg atgtgggcga
     2701 tgatcgagaa gttgcggatg cggtcgaggc cgatgtccaa agcgggagcg tccatggcga
     2761 agacgtgatg ctatcggcgg ccgtcacgcg accagagggc cgcgtccagg gggcccgtcg
     2821 cggcccccag gatcagctcc cgagcgcgcc gaagtcgatg ttgcagccga ccagcgccaa
     2881 cgcgaaggcc accgagcctt cttggctgat ctgtcccgtc gtgatgccct ggatgtccgc
     2941 gtcggtgagc ttgccgtcca gtgccttgac cgcacagtcg atctgctcgt cggtcaccgt
     3001 gccgccgctg ccggccgcga tcagttgcag gagctgacgg aggccttcgg cgccgccctt
     3061 cacgctgatg gacggcgagt cgccatcgtc gctgctgccg tcgtcatcgc tgctcgacgt
     3121 gatgccgcag ttcttctcca gatactcggc ccgctcgtcg tcgaggtcgt cggtgttgcg
     3181 ctcgctga
//
LOCUS       DAORNF010000371         3181 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_22961_length_3181_cov_1.390275, whole genome shotgun sequence.
ACCESSION   DAORNF010000371 DAORNF010000000
VERSION     DAORNF010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3181
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22961_length_3181_cov_1.390275"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>858)
                     /locus_tag="PLV68_13965"
     CDS             complement(<1..>858)
                     /locus_tag="PLV68_13965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRE02405.1"
                     /translation="PAEWKLVDENQAASMCYTSGTTGNPKGVVYSHRSTFLHTMGAMT
                     VDSLGACEADVILPVVPMFHANAWGLAHAAVASGASLVMPGPDLSGKAIADLIVNERV
                     TVAAGVPTIWMAVLPELQGRDTSSLRVVPCGGSAVPQALSEGYRATIGMPIRQAWGMT
                     ETSPIASSNFLRASELLLPVEEQADLNTAVGIISFGVDFRVVEPDTTTSVAWDGESSG
                     ELQCQGPWIARDYYNDPRSGESFTADGWLKTGDVAVVDPGGRIRLVDRTKDLIKSGEV
                     RPGHKLPAV"
     assembly_gap    859..990
                     /estimated_length=132
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<991..>1242)
                     /locus_tag="PLV68_13970"
     CDS             complement(<991..>1242)
                     /locus_tag="PLV68_13970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRE02406.1"
                     /translation="RTDYGEWADRTRRLGTVLDNLGISPDGRVATFAWNTARHLELYF
                     AAPCSGRVLHTLNIRLFPEQLTYIVNHAEDEVIFVDRSL"
     assembly_gap    1243..1289
                     /estimated_length=47
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1454..>1680
                     /locus_tag="PLV68_13975"
     CDS             1454..>1680
                     /locus_tag="PLV68_13975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02407.1"
                     /translation="MEPVRASSSTGRQAKLLLTAFGLLVVCTQVGAVLAPTLFDRSPA
                     LLLALSSRMRHLLLAVPAGINPVAYAVIGFVR"
     assembly_gap    1681..1851
                     /estimated_length=171
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1852..2150
                     /locus_tag="PLV68_13980"
     CDS             <1852..2150
                     /locus_tag="PLV68_13980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02408.1"
                     /translation="IACMLVGQRGMRAKRFGIWLSAGIAFRLAWLWVAGKAFEEPLKA
                     VLRFIEKYQWWLVAAFLLLSVAQSMRKTARSPAPDVTDLSDATDLTEPGGDDVG"
     gene            complement(2179..2985)
                     /gene="dapB"
                     /locus_tag="PLV68_13985"
     CDS             complement(2179..2985)
                     /gene="dapB"
                     /locus_tag="PLV68_13985"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HRE02409.1"
                     /translation="MSTTTTSATESAPIRVGVIGAGGRMGRTICAGIAADPAFVLVAA
                     IDPHVAGETIAGVTVAGELDALTAAGAQVAIDFTVVSAARVSVPWLAAHGIHAVVGTT
                     GFSDDDVGSFRAAFTTSNCLIASNFSITAVLMMRFAEMAAPYFETAEIIELHHNGKAD
                     APSGTAVTTAKRMAAASPEWAADPTKHEVYPGARGGIGPTGIRIHSVRLRGLQAHQEV
                     ILGTVGQSLTIRQDSYDNSSYLPGISLACRAIGEHPGVTLGLEPLLDLGS"
     gene            complement(2982..>3181)
                     /locus_tag="PLV68_13990"
     CDS             complement(2982..>3181)
                     /locus_tag="PLV68_13990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02410.1"
                     /translation="MARAAGLLTTTGRVRPVAEQVARWDAVTHDDVRRVIDRVCTQPV
                     TSVALGPVAANRLDLGIPSGR"
BASE COUNT          418 a          947 c          987 g          479 t
ORIGIN      
        1 agacggccgg gagcttgtgg ccgggccgaa cttcaccgct cttgatcagg tccttggtgc
       61 ggtcgaccag ccggatgcga cccccggggt cgacgaccgc gacgtcgccc gtcttcagcc
      121 aaccgtcggc ggtgaagctc tcgcccgagc gcgggtcgtt gtagtagtcc ctggcgatcc
      181 acggcccctg gcattgcagc tcgccgctgg actcgccgtc ccaggccacc gacgtggtgg
      241 tgtcgggctc gactacgcgg aagtcgacac cgaaggagat gatgccgacc gcggtgttga
      301 gatcggcctg ttcctcgacc ggcaacagca attcggaggc gcgcaggaag ttgctggacg
      361 cgatcgggct ggtctcggtc atgccccacg cctgacggat gggcatgccg atggtggccc
      421 ggtagccctc gctgagcgcc tggggcacgg ccgagccgcc gcagggcacc acgcgcaggc
      481 tgctggtgtc gcggccctgc aactcgggga gcaccgccat ccagatcgtc ggtacgccag
      541 cggccaccgt gacccgctcg ttgacgatca ggtcggcgat cgccttaccc gacaggtcag
      601 gaccgggcat caccaacgat gcaccggaag cgaccgcggc gtgggccaga ccccaggcgt
      661 tggcgtggaa catcggcacc accggcagga tcacgtcggc ctcgcacgca cccaacgagt
      721 cgaccgtcat cgcccccatc gtgtgcagaa acgtgctgcg gtggctgtag acgacgccct
      781 tcgggttgcc cgtggtgccg ctggtgtaac acatgctcgc ggcctggttc tcgtcgacca
      841 gcttccactc ggcaggctnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gcagcgatcg gtcgacgaag atcacctcgt
     1021 cctcggcatg gttgacgatg taggtgagtt gctcggggaa caggcggatg ttgagggtgt
     1081 gcaacacccg gccgctgcac ggtgcggcga agtacagctc caagtggcgg gccgtgttcc
     1141 acgcgaaggt ggcgacgcgg ccatcggggc tgatgccgag gttgtcgagc acggtgccga
     1201 ggcggcgggt gcgatcggcc cactcgccgt agtccgtccg ctnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnng ctcggcgcga ttgaagagat gaacgatcga
     1321 caacggtgtg tcttgcatca agccgagcat ggcaaccccc agtgattttc aggcaacgtg
     1381 tgtgagcgcg cagcgtgttc gtggtcccgc tgattcccca gtcaggtggg catacgttac
     1441 tgtcaccggc gtcgtggaac ctgttcgagc ctccagttcc accggtcgcc aggccaagtt
     1501 gttgctcacc gcgttcgggc tgctggtggt ctgcacgcag gtgggtgccg tgctggcacc
     1561 gacgttgttc gaccgcagtc cagctctgct gctcgcactc agctcacgga tgcgccacct
     1621 gctgctggcc gtgcccgcag gcatcaaccc ggtcgcgtat gcggtgatcg ggttcgtccg
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nacatcgcct
     1861 gcatgctcgt cggccagcgc ggcatgcggg ccaagcggtt cggcatctgg ctgtcggccg
     1921 gcatcgcctt ccgcctggct tggctgtggg tagcgggcaa ggcgttcgaa gagccgctca
     1981 aagcggtgct gcggttcatc gagaagtacc agtggtggct ggtggcggcg ttcttgctgc
     2041 tgtccgtcgc ccagtcgatg cgcaagaccg ctcgctcgcc agcgcccgac gtcaccgatc
     2101 tcagcgacgc caccgatctc accgagccgg ggggcgacga cgtcgggtga tcgtcggtcc
     2161 agccagcacc ggcgttggtc agctaccgag gtcgagcagc ggttcgagcc cgagggtgac
     2221 gccggggtgc tcgccgatgg cacggcaggc caggctgatg ccgggcaggt agctggagtt
     2281 gtcgtagctg tcctggcgga tggtgaggct ctgcccgacc gtgcccagga tgacctcttg
     2341 gtgagcttgc agcccgcgca ggcgcaccga gtggatgcgg atgccggtcg gcccgatacc
     2401 gccgcgcgca cccgggtaga cctcgtgctt ggtcgggtcg gccgcccact cgggcgacgc
     2461 ggccgccatt cgcttggcgg tggtgaccgc cgtacccgac ggggcgtcgg ccttgccgtt
     2521 gtggtgcaac tcgatgatct cggcggtctc gaagtacggc gcggccatct cggcgaagcg
     2581 catcatcaac acggccgtga tcgagaagtt cgacgcgatc aggcagttgc tggtggtgaa
     2641 cgcggcgcgg aacgagccga cgtcgtcgtc gctgaacccg gtggtgccga ccacggcatg
     2701 gatgccgtgt gctgccagcc atggcaccga cacccgcgct gccgacacca cggtgaagtc
     2761 gatcgccacc tgcgcgcctg ccgcggtcag cgcatcgagc tcacccgcca ccgtcacccc
     2821 tgcgatcgtc tcgcctgcga cgtgcgggtc gatcgctgcc accagtacga aggccggatc
     2881 ggcagcgata ccggcgcaga tcgtgcgccc catccgcccc ccggcgccga tcacccctac
     2941 tcggatcggt gcggattcgg tcgcgctcgt ggtggtcgta ctcatcgccc cgacggtatc
     3001 cccaggtcga ggcggttggc ggcgaccggg ccgagcgcga cggatgtgac cggctgcgtg
     3061 cagacccggt cgatcacccg gcgcacgtcg tcgtgggtca ccgcgtccca gcgggcgact
     3121 tgctcggcca ccggacgcac ccggccggtc gtggtcaaca gtccggcggc gcgggccatc
     3181 c
//
LOCUS       DAORNF010000372         3179 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_22980_length_3179_cov_1.924136, whole genome shotgun sequence.
ACCESSION   DAORNF010000372 DAORNF010000000
VERSION     DAORNF010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22980_length_3179_cov_1.924136"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /gene="nadE"
                     /locus_tag="PLV68_13995"
     CDS             <1..435
                     /gene="nadE"
                     /locus_tag="PLV68_13995"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF014586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HRE02411.1"
                     /translation="RIRGLTLMAMSNKFGWMVLTTGNKSEMAVGYFTIYGDSVGGYAV
                     IKDVFKTRVYDLCRYYNRRAGAEIIPEAVIVKPPSAELRPDQRDDQSLPPYEVLDPIL
                     AMYVEDAPRPRSSPRATTRRWCVASPGWSTSTSTSAANARRE"
     gene            complement(507..1694)
                     /locus_tag="PLV68_14000"
     CDS             complement(507..1694)
                     /locus_tag="PLV68_14000"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRE02412.1"
                     /translation="MFAARLDDQYTAEVRGSVGLLTVAKIAGNSAARFAPPLIATIAS
                     GLDVSLTTVGTALAIADFSGLLGPWVARITDAWPRRTAMAIGLAVMSVGAIVCASAVG
                     LVTLATGLVLIVVGKLALDVNVLSWVSDRVPYARIGRVVGLTETAWALALLSGVVIMA
                     LVAGVASWRWSYVVAAVGLAAVGGALIARLAPEGQHAHLERRTESGPRAPFGLGWLVA
                     LGTFALCAGSQAVFVTFGSWLEDDFGFTTTGIAAVVFSLGVVELIAASATVRYADLWG
                     KERAVLWGTAAMVPAAALLAAGSTVLAAGLIGLALFIASFEFAIVTTWSLAASLVPAS
                     ASAGMGILLAAATFGRALMSPATTAAYTSHGLWLVGVIAVAASLVSVGAFVAYIRLAA
                     RRS"
     gene            1758..2963
                     /locus_tag="PLV68_14005"
     CDS             1758..2963
                     /locus_tag="PLV68_14005"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRE02413.1"
                     /translation="MSDFEASMVPGVLWFVDSPQFGGAWEPGGERQVVGWVASREPIS
                     QLRLTARHDSSRILSADPQLAPRPDVDNAFGLAAIGFTFDLSAPLREATAVRFHVGGR
                     DVAIGVPHLADFGVGADDGRGDGYAAFKRRRIVPLLVCERCGGSFAETEGATEATRAV
                     TCTGCGWRVWHGSGPLDFLTDEVWQSAGLVETANVSSNRYDGTMLNLIHSLPDGLILD
                     CGAGSQGIAFPNVVNLEVVDYPSTDVKAVGERLPFADASFDAVVSFAVLEHVRDPFAC
                     AAEMLRVLRPGGTLYAQVPFLQPVHGYPNHYYNMTMQGLTNLFGQIDVVQSGVLDFGQ
                     PLFALSWFLNRYLDGLPPATRQQFAAMTVAELAAAPDARLGAPIVTDLDDDARATLSC
                     TNYVIGTRR"
     gene            2998..>3179
                     /locus_tag="PLV68_14010"
     CDS             2998..>3179
                     /locus_tag="PLV68_14010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HRE02414.1"
                     /translation="MALIVQKFGGTSVADPDRMRSVAEHVAFTRRHGNDVVVVVSAMG
                     KTTDNLISLATQVSSTR"
BASE COUNT          528 a         1114 c         1063 g          474 t
ORIGIN      
        1 cgcatccgtg gcctgacgct gatggcgatg tcgaacaagt tcggctggat ggtgctgacc
       61 accggcaaca agagcgagat ggcggtcggc tacttcacca tctacggcga cagcgtcggt
      121 ggctacgccg tgatcaagga cgtgttcaag acccgggtct acgacctgtg ccgctactac
      181 aaccgccgcg ccggagccga gatcatcccc gaagcggtga tcgtcaagcc gccgtcggcc
      241 gagctgcgcc ccgatcagcg cgacgaccag tcgctgccgc cctacgaggt gctcgatccg
      301 atcctggcga tgtacgtcga agacgcaccg cggccgagat catcgccgcg ggctacgacg
      361 aggcgctggt gcgtcgcatc gcccggctgg tcgacgtcaa cgagtacaag cgccgccaat
      421 gcccgccggg agtgaaggtg agcgcgaagg cgttcggcaa ggaccgccgc ctgccgatca
      481 ccacccgcta cgccggttga tccgagtcaa gagcggcgtg cggcgaggcg gatgtaggcc
      541 acgaacgcgc cgacgctgac cagtgaggcc gcgaccgcga tcacgcccac cagccacagc
      601 ccgtgagagg tgtaggccgc ggtcgtcgcc ggcgacatca acgccctgcc gaacgtggcc
      661 gcggcaagca ggatgcccat gcctgccgat gccgatgcgg gcaccagcga cgcggcgagc
      721 gaccacgtcg tgacgatggc gaactcgaac gaggcaatga acagcgccag gccgatcaga
      781 ccggccgcca acacggtgct gcccgcggcc agcagcgccg cggcgggcac catcgctgcc
      841 gtgccccaca gcaccgcccg ttccttgccc cacaggtcgg cgtaacgaac ggtggccgac
      901 gcggcgatca actcgaccac gccgaggctg aacaccacgg cggcgatacc ggtggtggtg
      961 aacccgaagt cgtcctccaa ccatgagccg aaggtgacga acaccgcctg cgatccggcg
     1021 cagagcgcga atgttccaag tgcaacaagc cagccgagcc cgaacggggc gcgaggtccc
     1081 gactcggtgc ggcgttccag gtgggcatgt tggccctcgg gcgcgaggcg ggcgatgagc
     1141 gcgccaccca ccgcagcgag gcccacggcg gcgaccacgt aactccagcg ccacgaggcg
     1201 acgcccgcga ccagggccat gatcaccacg ccggagagca gcgccaacgc ccatgcggtc
     1261 tcggtcaggc cgaccacacg gccgatccgc gcgtacggca ctcgatcgct gacccagctc
     1321 aacacgttga cgtcgagggc cagcttgccg accacgatca gcaccagccc ggtcgccaac
     1381 gtcaccagcc ccacggccga cgcgcacacg atcgccccga cggacatgac cgccaggccg
     1441 atcgccatcg cggtgcggcg cggccaggca tcggtgatgc gggcgaccca cggtccgagc
     1501 agcccgctga agtcggcgat cgccagcgcg gtgccgacgg tagtgaggct gacgtcgagc
     1561 ccgctggcga tcgtggcgat caacggcggg gcgaaccgcg cggcactgtt gcccgcgatc
     1621 ttggcgaccg tcagcagccc gaccgaccca cggacctccg cggtgtattg atcatccagc
     1681 cgagctgcaa acacaacctc cgaggctacg gccaccggac cctcggccag ccggtcagac
     1741 gggtacgctc gggctccgtg tccgacttcg aggcttcgat ggttccgggg gtgttgtggt
     1801 tcgtcgattc gccgcagttc ggaggggctt gggagcccgg gggagagcgg caggttgttg
     1861 gttgggttgc atcgcgggag ccgatctcgc agttgcggtt gacggcgcgt catgattcgt
     1921 cgcggatcct gagcgccgat ccccaactgg cgccgcgtcc cgacgtcgac aacgcgttcg
     1981 gtctcgccgc gatcggcttc acgttcgacc tctccgcccc gctgcgcgag gcgacggccg
     2041 tgcggttcca tgtgggcgga cgcgacgtgg cgatcggtgt gccgcacctg gccgacttcg
     2101 gtgtgggcgc cgacgacggg cgcggcgacg gctatgccgc gttcaaacgg cggcgcatcg
     2161 ttccgctcct cgtatgtgag cgctgcggtg gcagcttcgc cgagaccgag ggcgccactg
     2221 aggccacacg cgctgtcacc tgcacgggct gcggctggcg ggtctggcac ggctctggcc
     2281 cgctcgactt cctgaccgac gaggtgtggc agtcggcggg cctggtcgag acggcgaacg
     2341 tctcgtccaa ccggtacgac ggcacgatgc tcaacctgat ccactcgcta cccgacggat
     2401 tgatcctcga ctgcggcgcg gggtcgcagg gcattgcctt tccgaacgtc gtcaacctcg
     2461 aggtggtcga ctacccgtcc accgacgtca aggcggtcgg cgagcggttg cccttcgccg
     2521 acgccagctt cgacgcggtc gtgtcgttcg cggtgctcga acacgtgcgt gacccctttg
     2581 cctgtgcggc ggagatgctg cgggtgttgc gccccggtgg cacgttgtac gcccaggtgc
     2641 cgttcctgca gccggtgcac ggttacccga accactacta caacatgacg atgcagggtc
     2701 tgaccaacct gttcggtcag atcgacgtcg tccagtcggg ggtgctcgac ttcggtcagc
     2761 cgctgttcgc gctgtcgtgg ttcctcaacc gctacctcga cgggttgccg cccgcaacgc
     2821 gccaacagtt cgcggcgatg accgtggccg aactcgctgc cgcgcccgac gctcggctcg
     2881 gcgcaccgat cgtgaccgac ctcgacgacg atgcgcgggc cacgttgagc tgtaccaact
     2941 acgtgatcgg tacgcgtagg tagcgcttat cgcccgggtc ggtatcgttt cggacctgtg
     3001 gccctgatcg tccagaaatt cggcggcacc tcggttgccg atccagaccg catgcgctcc
     3061 gtcgccgaac acgtggcctt cacccgccgc cacggcaacg acgtcgtcgt cgtggtcagc
     3121 gcaatgggca agaccaccga caacctcatc tcgctcgcca cgcaggtgtc cagcacccg
//
LOCUS       DAORNF010000373         3165 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_23109_length_3165_cov_1.545981, whole genome shotgun sequence.
ACCESSION   DAORNF010000373 DAORNF010000000
VERSION     DAORNF010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3165
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23109_length_3165_cov_1.545981"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="PLV68_14015"
     CDS             complement(<1..180)
                     /locus_tag="PLV68_14015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02415.1"
                     /translation="MGKAPTRSHTVYFLGAGFSKGYGLPLTSELLQCLEELADRPEGK
                     FLNDGQQLSDRLKAVF"
     assembly_gap    248..401
                     /estimated_length=154
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<402..1964)
                     /locus_tag="PLV68_14020"
     CDS             complement(<402..1964)
                     /locus_tag="PLV68_14020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02416.1"
                     /translation="MSIPNIPGYIEARRPASLEDLWERPAGTFPDELWPAKALRQVVP
                     DPIGWLAVSEHFFTPEEHGGIGCVLVHPDDRDAALVEVTWVGDDLGTASIWESADGQG
                     FDAGLATDSPRLEFFVQARTPTGARLPTVDVSHPFLWYWDAFPVTNGWRYLNHAGRER
                     ELIRFTVGREHWRVEVRALEFRQFLSACGRDALIQVDYVPKTAASGFERVDSEFQSEW
                     AHFDFVATSDRLMGDRPGFSRLLGQYVVLGVRTAALPRFEERREDRDYPSFIYGMDPA
                     TGQALRHSCDPSTLGTYFDKDNTRLHFLTPINFRREVLQPYAAEPHRYRLSRTRLSCL
                     DLWGLDLSINTAGLVDAYLGDLGQRLPSDEWGHWLSYNVPPEGKMDEGRFRRDFLNQW
                     ASSADPPEDLRQAKNRAAEISSRVLGGPIWRELDEDTEAQWQSLVGPLSDDPTALGPC
                     LLLMTKVLVDGIDPTPLKGYLDDAAKGELSLSLLERFADRIGGTSSHVEPLRALQEFR
                     SKGGVAHLAGTGR"
     gene            complement(1961..>2151)
                     /locus_tag="PLV68_14025"
     CDS             complement(1961..>2151)
                     /locus_tag="PLV68_14025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02417.1"
                     /translation="LPADLIEAWDDESLDDAASLPSPGVLAAEIVEELEAALAQFTEL
                     AASLPVDGDDSAETLHDS"
     assembly_gap    2152..2161
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2162..>2471)
                     /locus_tag="PLV68_14030"
     CDS             complement(<2162..>2471)
                     /locus_tag="PLV68_14030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HRE02418.1"
                     /translation="RLPTGIFYAQGVKANVLFFDRKPAAADPWTKQTWVYDLRTNMHF
                     TLKTKPLRAEDLADFVNAYSPDDRSRRVDSERFRCFTHDELVARDKANLDITWLRDES
                     L"
     assembly_gap    2472..2621
                     /estimated_length=150
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2622..>3165)
                     /locus_tag="PLV68_14035"
     CDS             complement(<2622..>3165)
                     /locus_tag="PLV68_14035"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HRE02419.1"
                     /translation="INEETWTSVDTDVKGDAYEAILQDGAADTKSGAGQYFTPRPLVR
                     AMMACLRPRPGETIADPACGTGGFLLAAHEWIVDHHVAEMDADERRFLRDEMVHGWEI
                     VDGTARLCAMNLLLHGIGTANGKSLVTVADALRADPGVRVDVIAANPPFGRKSSMTMV
                     NAAGETERDDLVIVRDDFWAS"
BASE COUNT          478 a          910 c          968 g          495 t
ORIGIN      
        1 gaacacagcc ttcagccgat cggagagttg ctgcccgtca ttcagaaact tgccttcggg
       61 ccggtccgcc aactcctcga ggcactgcaa gagctcagag gtcaatggaa ggccatagcc
      121 cttggagaat ccagcaccga ggaagtacac ggtgtgggag cgggtcggag ccttacccat
      181 ggatcagcct cgacctaccg gcgaccagca aggggcgcga cgacccaatg ttcctcgggg
      241 tccgatcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nccggccagt tccggcgaga
      421 tgggcgactc cgcccttcga ccggaactcc tggagggcac gtagcggctc gacgtgggag
      481 ctcgttcctc cgatgcgatc cgcgaaccgt tccaggaggc tgaggctgag ttcccccttg
      541 gctgcgtcgt ccaggtagcc cttgagcggt gtggggtcga ttccatccac gagcaccttg
      601 gtcatcagga gtaggcacgg gccgagtgcg gtcgggtcgt cactcagggg tcccacgagc
      661 gactgccact gagcttcggt gtcctcgtcg agttctcgcc agatcgggcc accgagcacg
      721 cgactcgaga tctcggcggc tcggttcttt gcctgacgca ggtcttccgg cgggtcggcg
      781 gacgaggccc actggttgag gaagtcgcgt ctgaaccgcc cttcgtccat cttcccctcg
      841 ggcggaacgt tgtaggacaa ccagtggccc cactcatccg atggaaggcg ctggccgagg
      901 tctccgagat acgcgtcgac gagcccagcg gtgttgatcg acaggtcgag gccccacaga
      961 tctaggcacg aaagccgtgt tcgactgagt cgatagcggt gaggttctgc cgcgtagggc
     1021 tgaaggactt cgcgacggaa gttgatcggg gtcaggaagt ggaggcgggt gttgtccttg
     1081 tcgaagtagg tgcccagggt cgatgggtcg cacgagtgcc gtagcgcctg gccggtcgct
     1141 ggatccatcc cgtagatgaa cgacggatag tcgcggtcct ctcggcgctc ctcgaatcgc
     1201 gggagcgctg ccgtgcgcac gccgagaacc acgtactggc cgagtaggcg ggagaaccca
     1261 ggacggtcgc ccatcagccg gtcagacgtc gccacgaagt cgaagtgcgc ccattctgat
     1321 tggaactccg agtcaacccg ttcgaagcct gacgccgcgg tcttcggcac gtagtcgacc
     1381 tgaatgaggg cgtctcggcc gcacgccgag aggaactggc ggaactcgag cgcccggacc
     1441 tcgactctcc agtgctcacg gcccaccgtg aaccggatga gttcccgttc gcgtcctgcg
     1501 tggttgaggt acctccagcc gttcgtgacg gggaacgcgt cccagtacca caggaatgga
     1561 tgggagacat cgaccgtggg aagtcgcgcg ccggtgggcg tccgcgcttg cacgaagaac
     1621 tccagccggg gactgtctgt agcgagtcca gcgtcgaagc cctggccgtc tgcgctctcc
     1681 cagatcgagg ctgttccgag atcgtcgcca acccaggtga cctcgaccag agcagcgtct
     1741 cgatcgtcgg gatgaacaag cacgcaaccg atcccgccgt gttcctcagg cgtgaagaag
     1801 tgctccgata cagcgagcca gccgatcggg tcggggacga cctggcgcag cgccttcgct
     1861 ggccacagct cgtccgggaa cgtacccgca gggcgttccc acaggtcttc caacgaggct
     1921 ggacgacgtg cctcgatgta tccggggatg ttcggaatgc tcacgagtcg tggagagtct
     1981 cagcggagtc gtcgccatca accggcaggc tggccgcgag ctcggtgaac tgggcgaggg
     2041 cggcttcgag ttcttcgacg atctcggcgg cgagcacccc gggggacggg agcgatgcgg
     2101 cgtcgtcgag ggactcgtcg tcccaggcct cgatcaggtc ggcgggcagg cnnnnnnnnn
     2161 ncgagggact cgtcgcgcag ccaggtgatg tcgaggttgg ctttgtcgcg tgccaccagt
     2221 tcgtcgtggg tgaagcaccg gaaccgctcg ctgtcgaccc gtcgactgcg gtcgtccggc
     2281 gagtaggcgt tgacgaagtc ggcgaggtcc tcggcgcgga gcggcttggt cttcagggtg
     2341 aagtgcatgt tcgttcgcag gtcgtacacc cacgtctgct tcgtccacgg gtccgcggcg
     2401 gcgggcttgc ggtcgaagaa cagcacgttc gccttcacac cctgcgcgta gaagatgccg
     2461 gtgggcaggc gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncgacgccca gaagtcgtcg
     2641 cgcacgatca ccaagtcgtc gcgctcggtc tcgcccgccg cgttgaccat cgtcatcgac
     2701 gacttacgcc cgaacggcgg gttggcggcg atcacgtcga cccgcacgcc ggggtcggcg
     2761 cgtagcgcgt cggcgacggt cacgaggctc ttgccgttgg ccgtgccgat gccatgtagc
     2821 aacaggttca tcgcacacag ccgggccgtg ccgtcgacga tctcccagcc gtgcaccatc
     2881 tcgtcgcgca ggaaccggcg ctcgtcggcg tccatctcgg cgacgtggtg atcgacgatc
     2941 cactcgtgcg cggcgagcag gaacccgccg gtgccacagg ccgggtcggc gatcgtttcc
     3001 ccgggcctcg gccgcaggca ggccatcatc gcccgcacca acgggcgcgg cgtgaagtac
     3061 tgaccggcac ccgacttggt gtcggcagca ccatcttgga gaatcgcctc gtaggcgtcg
     3121 cccttcacgt cggtgtcgac cgacgtccag gtctcctcgt tgatc
//
LOCUS       DAORNF010000374         3157 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_23186_length_3157_cov_1.839458, whole genome shotgun sequence.
ACCESSION   DAORNF010000374 DAORNF010000000
VERSION     DAORNF010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3157
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23186_length_3157_cov_1.839458"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>310)
                     /locus_tag="PLV68_14040"
     CDS             complement(<1..>310)
                     /locus_tag="PLV68_14040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02420.1"
                     /translation="ARLSKYSYLVSLMPDQIIEELGLTVELARRRISSYTPVGDGGLL
                     IDNSDPAATDARLGADAAGWRDLYAATGAVAQRMFPTLLEPLRSRDAMRRHIGNDAAW
                     Q"
     assembly_gap    311..320
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<321..479)
                     /locus_tag="PLV68_14045"
     CDS             complement(<321..479)
                     /locus_tag="PLV68_14045"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRE02421.1"
                     /translation="MSERYDAVVVGGGHNGLVAACYLAAAGRSVAVLERTDAVGGAVT
                     SARVFPAYD"
     gene            549..>1519
                     /locus_tag="PLV68_14050"
     CDS             549..>1519
                     /locus_tag="PLV68_14050"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRE02422.1"
                     /translation="MSASGKTKTFPDMTTAIDPEVYARRWKILGVLCLSLIVIMVANS
                     SLNTALPAMAEDLDASASELQWMVDSYSLVFAGLLFTAGTLGDRFGRRIALQGGMTIF
                     LFGSIAASLSDSASGVIGWRAVMGLGAAFVMPSTLSLLTNVFPPHERPKAIATWAGVA
                     GGGAAFGPPLSGFLIEHFWWGSVFLINVPLLLIALVAGRMMLPESVDPHQVKVDVPGA
                     LLSIVAIGLAVYSIIEAPTHGWLSATTLLTMAGAVVAALVFAAWEHRTAHPMFELSLL
                     RDRRYAVAAVVIGLCFFAMFGLMFLMAQYLQLVLGYSALGAGLLMLP"
     assembly_gap    1520..1591
                     /estimated_length=72
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1592..2166
                     /locus_tag="PLV68_14055"
     CDS             <1592..2166
                     /locus_tag="PLV68_14055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02423.1"
                     /translation="AIGLLLLIMLRTDSHGWVAYFGMLPIMMGMATAMSPCTALIMSA
                     VPANRAGMGSASNDTSRELGGALGVAVLGSVVATTFKANIDSGVVPEAARAAVESGLP
                     GALKVAGALAEVNPTVAAGLMVDARTAFVDGLHIATVVGAVVVGITAVAAAAVLRHLP
                     ASAMRLAQEMAEEPAAGMGDDIEPGTASAL"
     gene            complement(2186..>3157)
                     /locus_tag="PLV68_14060"
     CDS             complement(2186..>3157)
                     /locus_tag="PLV68_14060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HRE02424.1"
                     /translation="IGEVDPIKVAEGRYLSDELTLHYGLVPRTNRGIFAINEIPDLAE
                     RIQVGLLNVLEERDVQVRGYKIRLPLDVMLLASANPDDYTNRGRIITPLKDRFGSQIR
                     THYPLEVETEVAIMRQEARPASVGDVHVTVPDYMDEVIATLSHLARESSHVNQRSGVS
                     VRLSVSNAEVLAANALRRGLRAGERDVVPRVCDLDAIAASTSGKIEIESMDEGREGSI
                     LQNLVRAAVLTVYKQRISHEQVRDVIAAFDDQGVVAHTGEDIASADLVALIDTVPALR
                     APVLALTDGNESPAAIAAAVEFVLEGLHLSKRLNKDSAGTHATFRAR"
BASE COUNT          423 a         1012 c         1066 g          574 t
ORIGIN      
        1 cctgccaagc cgcgtcgttg ccgatgtggc gtcgcatcgc atcgcggctg cgcagcggtt
       61 ccagcagggt cgggaacatc cgctgcgcga ccgcacccgt cgcggcgtac aggtcacgcc
      121 agcccgcggc gtccgcgccg aggcgggcat cggtggcggc tggatcggag ttgtcgatca
      181 gcaggccacc gtctccgacc ggcgtgtacg acgagatgcg gcgccgtgcg agttcgaccg
      241 tgaggcccaa ctcctcgatg atctggtcgg gcatcaggct gacgaggtag ctgtacttcg
      301 acagccgagc nnnnnnnnnn gtcgtaggcg ggaaacaccc gggccgacgt caccgcgccc
      361 ccgaccgcat cggtccgttc cagcacggcg accgaccgtc cggccgcggc cagatagcag
      421 gccgccacca gaccgttgtg tccaccaccc accacgaccg cgtcgtaccg ctcgctcatc
      481 gcgccgacgg taccagcgcg tctttcgcgc gacgcattgg cccaatctgg tcgtaccgtg
      541 cctcgtctat gtccgcatcc ggcaagacga agacgttccc cgacatgacg acggcgatcg
      601 atccagaggt gtacgcgcgg cgttggaaaa tcctcggcgt tctgtgcctc agcctgatcg
      661 tcatcatggt ggccaacagc agcctgaaca cggcgctgcc ggccatggcc gaagacctcg
      721 acgcctcggc cagcgaactg cagtggatgg tcgacagcta cagcctggtg ttcgccggcc
      781 tgctgtttac agccggcacg ctgggtgacc gcttcggtcg tcgcatcgcg ctccaaggcg
      841 gtatgacgat cttcttgttc ggctcgatcg cagcgtcgct gtcggactcg gcaagcggtg
      901 tgatcggttg gcgcgcggtg atgggcttgg gtgccgcgtt cgtgatgccc tccacgttgt
      961 cgttgttgac caacgtattc cctccccacg aacgcccgaa ggccatcgcc acctgggccg
     1021 gtgtggccgg tggcggcgct gccttcggcc cgccgctgtc ggggtttctg atcgagcact
     1081 tctggtgggg gtcggtgttc ctcatcaacg tgccgctgtt gctgatcgct ttggtcgccg
     1141 gacggatgat gttgcccgaa tcggtcgacc cgcatcaggt caaggtcgac gtgcccgggg
     1201 ccttgctgtc gatcgttgcg atcggtctcg ccgtgtacag catcatcgag gcgccgaccc
     1261 acggctggtt gtcggcgacc acgctgctca ccatggctgg cgcggtcgtc gcggccttgg
     1321 tgttcgctgc gtgggagcac cgcacggccc acccgatgtt cgagctgtcc ctgctgcgcg
     1381 atcgccgcta tgccgtcgcg gccgtcgtga tcggtctgtg cttcttcgcc atgttcgggc
     1441 tgatgttcct gatggctcag tacctccagc tcgtgctggg atactcggca ttgggggccg
     1501 ggctgttgat gttgccgatn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntggcgatcg ggctgttgct gctcatcatg
     1621 ctgcgcaccg actcgcacgg atgggtcgcc tacttcggga tgctgccgat catgatgggc
     1681 atggccaccg ccatgtcgcc ctgcaccgcc ttgatcatgt cggcagtgcc ggccaaccgg
     1741 gcgggtatgg gctcggcgtc caacgacacg tcacgcgagc tcggcggcgc gttgggggtg
     1801 gccgtgctgg gctcggtggt cgctaccacg ttcaaggcca acatcgactc cggggtcgtg
     1861 ccagaagcgg cacgcgccgc ggtcgagagt ggtcttccgg gtgcgctcaa ggtcgcgggt
     1921 gctttggccg aggtcaaccc gaccgtcgcg gcggggttga tggtcgatgc ccggaccgcg
     1981 ttcgtcgacg gtctgcacat tgcgaccgtg gtcggtgcgg tggtcgtcgg catcactgcc
     2041 gtggccgctg cggcagtgct gcgtcatctg cccgcgtcgg cgatgcgtct cgcgcaggag
     2101 atggccgagg aacctgcagc cgggatgggc gacgacatcg agccgggcac cgcgtcggcc
     2161 ctctgagccg gcgcctctca gaggctcagc gggcgcggaa tgtggcgtgc gtgcccgccg
     2221 agtccttgtt gagccgcttc gacaggtgca ggccttcgag cacgaactcg accgcagccg
     2281 cgatcgcggc gggtgactcg ttgccgtcgg tcagcgccag caccggcgcg cgcagcgcag
     2341 gcacggtgtc gatcagcgcg accagatcgg ccgaggcgat gtcctcaccc gtatgggcga
     2401 ccacgccctg gtcgtcgaac gcggcgatca cgtcgcgcac ctgctcgtgg gagatgcgct
     2461 gcttgtagac ggtgagtacc gcggcgcgga ccaggttttg gaggatcgac ccttcgcgcc
     2521 cttcgtccat cgactcgatc tcgatcttgc ccgaggtcga cgcggcgatc gcgtccaggt
     2581 cgcacacccg cggcaccacg tcgcgctcac ccgcgcgcag gccgcggcgc agcgcgttgg
     2641 cggccaacac ttcggcgttg ctgaccgaca gtcgcaccga cacaccgctg cgctggttga
     2701 cgtggctgct ctcgcgggcg aggtggctga gggtggcgat gacctcgtcc atgtagtcgg
     2761 gcaccgtgac gtgcacgtcg ccgacgctgg cagggcgggc ctcttggcgc atgatcgcca
     2821 cttcggtctc gacctcgagc gggtagtggg tgcggatctg gctgccgaag cgatccttga
     2881 gcggggtgat gatccggccg cggttggtgt agtcgtccgg gttggccgag gcgagcagca
     2941 tcacgtcgag cggtaggcgg atcttgtagc cgcgcacctg cacgtcgcgc tcttccagca
     3001 cgttgagcaa gccgacctga atccgctcgg ccaggtcggg gatctcgttg atggcgaaaa
     3061 taccccggtt ggtgcgcggg accaggccgt agtgcagcgt cagctcgtcg ctgaggtagc
     3121 gaccctcggc gaccttgatc gggtcgacct cgccgat
//
LOCUS       DAORNF010000375         3145 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_23297_length_3145_cov_2.020388, whole genome shotgun sequence.
ACCESSION   DAORNF010000375 DAORNF010000000
VERSION     DAORNF010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3145
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23297_length_3145_cov_2.020388"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..856
                     /locus_tag="PLV68_14065"
     CDS             <1..856
                     /locus_tag="PLV68_14065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02425.1"
                     /translation="WVSMLTRVYVTISTGQLAAAALADPYVSLVLAEQDIDTDRAASL
                     SALAFAGRASDGRSLEGLLVESVIRVKTLIASGADVTTALRSGEASLLRIVTTQVADA
                     GRTAVGTATVARPAATKWVRMLNPPSCSRCALLAGKVYRWNQGFRRHPHCDCVHIPST
                     ESIAGDLTTDPAAYFNSLTAADQDRYFTVAGAQAIRDGADIGQVVNARRRGAMYQANE
                     RSYTREGTTVRGFAGSRIGGTTERLSDARYSSVKTGRPMPETIYGNATDRAEAVGLLR
                     RYGYLVAA"
     gene            948..1628
                     /locus_tag="PLV68_14070"
     CDS             948..1628
                     /locus_tag="PLV68_14070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02426.1"
                     /translation="MSRPNHADDWSADPFLIPHRGRLAWLWPSGRITPDVSGGEGEGD
                     ADKKFTQADVDRIIQQRLDQWQRNNPPAPPADYQQIKDELATLKAAAGTDLDRANTRI
                     AALEQQLSGVQADLVKANGERDDAKLNLLGAAMKSAVTTAARAAANPDQVYALLPKDA
                     LTIGDDGQVKGAEEAVKKFLADNPHFVGKTGPRPDKGQGATGAPPSGREAGLAEAQRR
                     GWIKSETN"
     gene            1651..2361
                     /locus_tag="PLV68_14075"
     CDS             1651..2361
                     /locus_tag="PLV68_14075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02427.1"
                     /translation="MTDIHVSRETFQVENRSWLLGPTGTRPGENPSITLDISTFTPGV
                     HYPNGYIPSGTALGRLTANGKYGPYSGTTEEVQSITVGGSGLTSFTLTFSGQTTSSLA
                     AAATAEQVQLALEALSNIGAGNITVTGPAGGPWIATFVGGLADTNVAQMTSTPTGGSG
                     TVTVATVQAGGVGESADGRETAAMLLFSSVKVPDLLNTAIDVGAAGFVTGFVSLSRLP
                     FALDANGQADLAHIVFSD"
     gene            2387..>2831
                     /locus_tag="PLV68_14080"
     CDS             2387..>2831
                     /locus_tag="PLV68_14080"
                     /inference="COORDINATES: protein motif:HMM:NF015802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HRE02428.1"
                     /translation="MIFFDAPVAPDALTAFVRQVPNTASNALTALFPTRTYDDNEVEW
                     AEITHTNRTARYRSWDGRIHVSERDTGSTAKVKLPPLSSSLNRGEYERLQIEFARTQG
                     TFTEALERAIYNDAANLTGEIYNRMEEGWGDVLTDGKLSIDEDGFT"
     assembly_gap    2832..2925
                     /estimated_length=94
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2926..>3145
                     /locus_tag="PLV68_14085"
     CDS             <2926..>3145
                     /locus_tag="PLV68_14085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02429.1"
                     /translation="WNDKYIETNGVGAATFLSSQRIIRLLTQNKEVIDAVHGSTAGRT
                     MVTLAELRGILEAEGLPIPRAPYDASLRT"
BASE COUNT          561 a         1090 c          974 g          426 t
ORIGIN      
        1 gtgggtctcg atgttgaccc gggtgtacgt cacgatctcg acaggccagc ttgccgcggc
       61 ggcgctcgcg gacccgtacg tgtcgctggt actcgccgag caagacatcg acaccgaccg
      121 tgccgccagc ctcagcgcgc tggctttcgc cggacgcgcc agtgacggac gttccctcga
      181 agggctcctc gtcgagtctg tgatccgggt caagacgctc atcgcgtccg gtgctgacgt
      241 caccacggcg ctgcgatcgg gtgaggcgtc actgctgcgg atcgtcacca cgcaggtggc
      301 tgacgccgga cgcaccgcgg tcgggaccgc gaccgtcgcc cggcccgccg caaccaagtg
      361 ggtgcgcatg ctcaacccgc cgtcctgctc acgctgcgct ctgctcgcag gcaaggtgta
      421 ccgctggaac caagggttca gacggcaccc acactgcgac tgcgtccaca tcccatccac
      481 cgaatcgatc gccggggacc tcaccaccga ccccgccgcc tacttcaaca gtctcaccgc
      541 agccgatcag gacaggtact tcaccgtcgc tggcgcgcaa gcgatccggg acggcgccga
      601 catcggccag gtcgtgaacg cccgccgccg gggcgcgatg tatcaggcga acgaacggtc
      661 ctacacccgg gaagggacca cggtccgagg cttcgccggg tcgaggatcg gtggaacgac
      721 tgagcggctc agcgacgccc ggtactcgtc ggtgaagacg gggcggccga tgcccgagac
      781 gatctacggc aacgccaccg accgcgccga agcggtcggg ctgctgcgcc ggtacggcta
      841 cctcgtcgct gcctgacgag cccgccatga gaactccccg gagagcgggg tggtgcgcag
      901 acggccagcg cgaccccaac ggccggtcac tcctcggagg tccaaccatg tcccgtccca
      961 accacgccga cgactggtcg gccgacccgt tcctgatccc gcatcgcggc cgcctcgcgt
     1021 ggctgtggcc atccggtcgc atcaccccgg acgtgtccgg cggcgaaggc gaaggcgacg
     1081 ccgacaagaa gttcacccag gccgacgtcg accgcatcat ccaacagcga ctcgaccagt
     1141 ggcagcggaa caacccgcca gcgccgccgg cggactacca gcagatcaaa gacgaactcg
     1201 ccacgctgaa ggcagccgcg ggcaccgacc tcgatcgagc caacacgcgg atcgcggcgc
     1261 ttgaacagca actctcaggc gtgcaggccg acctggtcaa ggcgaacggc gagcgcgacg
     1321 acgccaagct caatctgctc ggcgccgcca tgaagtccgc ggtgaccacc gcggcgaggg
     1381 cagcagcgaa ccccgatcag gtgtacgcgc tgttgccgaa ggacgcactg accatcggcg
     1441 acgacggcca ggtcaagggt gcggaagagg ccgtgaagaa gttcctcgcg gacaaccctc
     1501 acttcgtcgg caagaccgga cctcgacccg acaagggtca aggggcaacc ggggcgcctc
     1561 cgtccggccg tgaggccgga ttggccgagg cgcaacgtcg cggctggatc aagtccgaga
     1621 cgaactgacc accccaagaa agcaggaatc atgaccgaca ttcacgtgtc gcgggaaacg
     1681 ttccaggtcg agaaccgttc gtggctgttg ggcccgaccg ggacccgtcc gggcgagaac
     1741 ccgtccatca ccctcgacat ctccaccttc acgcccggcg tgcactaccc caacgggtac
     1801 atcccatccg ggaccgcgct cggtcggctc accgccaacg gcaagtacgg cccgtactcg
     1861 ggcaccaccg aggaagtcca gtcgatcacc gtcggcggat ccgggctgac gtcgttcacg
     1921 ctgacgttct ccggtcagac gacgtcgtcg ctggccgctg ctgcgactgc cgagcaggtg
     1981 cagttggcgc tcgaggcgct gtcgaacatc ggcgccggga acatcactgt caccggcccc
     2041 gctggcggtc cctggatcgc gacgttcgtc ggtggtctcg ccgacaccaa cgtcgcgcag
     2101 atgacctcca cccccaccgg gggaagcggc accgtcaccg tcgccaccgt gcaggccggc
     2161 ggggtcggtg agtcggccga tggccgcgag acggcagcga tgttgctgtt ctcctcggtc
     2221 aaggtgcccg acctgttgaa caccgcgatc gatgtcggcg ccgccggctt cgtgaccggg
     2281 ttcgtgtccc tgtcccggct cccgttcgcg ctcgacgcca acggtcaggc cgacctcgcc
     2341 cacatcgtgt tcagcgactg agcgctgaag aaagagagcc aatgtcatga tcttcttcga
     2401 cgcaccggta gccccggacg ccctcaccgc cttcgtgcga caggtgccca acacggcgag
     2461 caacgcgctg accgcgctgt tcccgacgcg cacctacgac gacaacgaag tcgagtgggc
     2521 cgagatcacc cacaccaacc gcaccgcccg ctaccgctcg tgggacggcc gaatccacgt
     2581 ctctgagcgt gacaccggca gcaccgccaa ggtcaagttg cctccgctga gctccagcct
     2641 caaccggggt gagtacgagc gcctgcagat cgagttcgct cgcacgcagg gcaccttcac
     2701 cgaggcgctc gagcgggcga tctacaacga cgcagcgaac ctcaccggtg agatctacaa
     2761 ccggatggag gaaggctggg gcgacgtgtt gaccgacggg aagctgtcga tcgacgagga
     2821 cggcttcacc annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncgtgg aacgacaagt
     2941 acatcgagac caacggtgtc ggtgcggcca cgttcctgtc ctcgcagcgg atcatccgtc
     3001 tgctcacgca gaacaaggag gtcatcgacg ccgtgcacgg atcgacggcc ggccgcacga
     3061 tggtcaccct ggccgagttg cgcggcatcc tcgaagccga ggggctgccg atcccgcgtg
     3121 ccccgtacga cgcgagcctg cggac
//
LOCUS       DAORNF010000376         3141 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_23345_length_3141_cov_1.657485, whole genome shotgun sequence.
ACCESSION   DAORNF010000376 DAORNF010000000
VERSION     DAORNF010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3141
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23345_length_3141_cov_1.657485"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    231..350
                     /estimated_length=120
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <351..656
                     /locus_tag="PLV68_14090"
     CDS             <351..656
                     /locus_tag="PLV68_14090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02430.1"
                     /translation="HPPTKEQRQMLHLDHNPETFPAAAVAASAVDPVLTVDQAFRLQA
                     LLDQHQWDQVWHACLDANRGGATPKSVVAGAMARAREQLGLPPTTTESPDLSSLDGS"
     assembly_gap    729..747
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            948..>1141
                     /locus_tag="PLV68_14095"
     CDS             948..>1141
                     /locus_tag="PLV68_14095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02431.1"
                     /translation="MSEKTFGVRLQANVTSFVQGIERAERETKKFSENAGANFERVGS
                     KVQSMGSKATRMLTVPILAAG"
     assembly_gap    1142..1161
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1162..>2573
                     /locus_tag="PLV68_14100"
     CDS             <1162..>2573
                     /locus_tag="PLV68_14100"
                     /inference="COORDINATES: protein motif:HMM:TIGR01760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HRE02432.1"
                     /translation="SRMVGLADVAADEVDGLKKSVLDLSRATGRGPQELANALYEAAS
                     SGLDTAQAMDAVTQAARGAAAGLGSSEEVVSLVASATTAYGAANMDASRALDILTQTI
                     REGRADPEELAGTLGRILPVAASLGVSFEEVGGATAYLSNVFGNTNRTVTAMSGIMVK
                     LLSPTAQGRQALLDMGTSMEQLSAAIDERGLMGALDLLRERGFGGNQRALRALFDDVE
                     AFQGITALLGADQEQLAGIMERTADSAGAASSAFATATETDGFRMRQAWADIQTAMIA
                     AGDIILPIVAGIAGAIGKVAGVFAALPEPVQTVVVAFLGLVAAAGPVLMVAGSLIKNY
                     ALLHGAATKAAGSTGLFARAAGAAATGLGALAVGLGVVELFDQLTGRTGDANDELNVL
                     VSTLSKVGKLELPEGQTMSEGVSEITAAFTELALKIQDTKSSWQIAKDMADWQTDQEF
                     NLKQMSDAFDQVFNTSPDMA"
     assembly_gap    2574..2635
                     /estimated_length=62
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2636..>3141
                     /locus_tag="PLV68_14105"
     CDS             <2636..>3141
                     /locus_tag="PLV68_14105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02433.1"
                     /translation="LDGWGLTNDVISDWNVRTHNAIDAQDALTGSVGEAGTALDAEIQ
                     AILDGADATDAKAQADEAARQELDELTDALDRHIAALQDDLEAQMAIVEFARGAVDAQ
                     YALARAQDSYARTLEELPGKLDAIAKSQDSTAEKQRRVNELYRAGAEDAAGVADAIVE
                     AVNQATLA"
BASE COUNT          500 a          951 c         1037 g          432 t
ORIGIN      
        1 cgttcgcggc gtcgttcttc gtcaccgccg agccgaacct caaggctgtg gtggcctgac
       61 cgtggctggg gagtccacgg tcgaggacct cctcgcggaa gcagcggccg cgccacggga
      121 agccgtggca cgggtgctgc tccgccagga cctcgttcgc cgccacgccg aactcgacgc
      181 acggtacgcg gcagcgctcg agcacgaccg ctggcacaac gacggcccga nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn catccgccga
      361 caaaggagca gcgccagatg ctccatctcg accacaaccc ggagacgttc ccggcggccg
      421 cggtcgccgc ctcagcggtc gatccggtgc tcactgtcga tcaggcattc cggctgcaag
      481 cgctgctcga ccagcaccag tgggaccagg tgtggcacgc gtgcctcgac gcgaaccgag
      541 gtggtgcgac cccaaaatcg gtggtcgctg gagcgatggc ccgcgcgaga gagcagttag
      601 ggctgccgcc caccactacc gagtcccccg atctgtcctc cttggacggg tcgtaacccc
      661 gggcgacccg ctctggcttg aagaggacct cgacgccgcg ctcgagtgga tggagtggca
      721 gtcgggacnn nnnnnnnnnn nnnnnnnccg agagtcgagt cgttcgaccc tgacggtccg
      781 gactacgagg ccgaggcgtg gcggtgccgt gcctgccagg tgcgtgacga agagatgcgc
      841 gactggcgcg agaacgcggg cgatgaccag tccgcgttcg acgggctgca cttcgcggtg
      901 aacgagatcg tcggcggacc ctgatcaagc gaagggggtg attggccttg agtgagaaga
      961 cctttggtgt ccggctgcag gccaacgtca cctcgttcgt gcaaggcatc gagcgcgcgg
     1021 agcgcgagac caagaagttc tcggagaacg ccggcgcgaa cttcgagcgc gtcggctcga
     1081 aagtccagtc gatggggtcg aaagcgaccc gcatgctgac cgtcccgatc ctcgcggcgg
     1141 gnnnnnnnnn nnnnnnnnnn ntctcgcgga tggtcggcct tgccgacgtc gccgccgacg
     1201 aggtggacgg tctcaagaag tccgtcctcg acctctcccg agcgactggc cgcggtccgc
     1261 aggaactcgc gaacgcgctg tatgaggcgg cgtcgtcggg tctggacacg gctcaggcga
     1321 tggacgctgt gacgcaggcg gcacgtggcg cggcggcggg gctcgggtcg tctgaggagg
     1381 tcgtgtcgtt ggtggcgtcc gcgacgaccg cgtacggcgc agcgaacatg gatgcgtccc
     1441 gcgccctgga catcctgacc cagaccatcc gtgagggccg cgccgacccg gaagagctcg
     1501 ccgggaccct gggccgcatc ctgcccgtcg cggccagcct cggagtgtcg ttcgaggagg
     1561 ttggcggcgc caccgcgtac ctgtcgaacg tgttcggcaa caccaaccgg accgtcaccg
     1621 cgatgtccgg gatcatggtc aagctcctgt caccgacagc gcagggccgc caagcgctgc
     1681 tcgacatggg cacgtcgatg gagcagttgt ccgctgcgat cgacgagcgc ggcctgatgg
     1741 gcgctctcga tctgctgcgc gaacgcggct tcggcgggaa ccagcgggcg ttgcgtgccc
     1801 tcttcgacga cgtcgaagcc ttccagggga tcaccgctct gttgggtgcg gatcaggagc
     1861 agttggccgg gatcatggag cgcaccgccg actcggccgg tgcggcgagt tcggcgttcg
     1921 cgaccgcgac ggagacggac gggttccgga tgcgtcaggc atgggccgac attcagacgg
     1981 cgatgatcgc ggccggcgac atcatcctgc cgatcgtcgc cgggatcgct ggcgccatcg
     2041 gcaaggtcgc cggagtgttc gcagcactgc cagaaccggt gcagaccgtc gtggtcgcgt
     2101 tcctcggcct ggtcgccgca gcgggcccag tcctgatggt cgccggatcg ctgatcaaga
     2161 actacgcgct cctgcacggc gcggccacga aggccgcagg atcgacgggg ctgttcgcac
     2221 gcgccgcggg tgccgcggcg accggattgg gtgcgctcgc tgtcgggttg ggtgtcgtcg
     2281 agttgttcga ccaactgacc ggccgcaccg gcgacgcgaa cgacgaactg aacgtgctgg
     2341 tcagcacctt gagcaaggtc ggcaagctcg agctccccga ggggcagacg atgtcggaag
     2401 gggtgagcga gatcaccgct gccttcacgg agttggccct gaagatccag gacaccaaga
     2461 gttcgtggca gatcgcgaag gacatggccg actggcagac cgaccaggag ttcaacctca
     2521 agcagatgtc ggacgcattc gatcaggtgt tcaacacgtc gccggacatg gcgnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnggctc
     2641 gacggttggg gactcaccaa cgacgtgatc tccgactgga acgtccgcac ccacaacgcg
     2701 atcgacgcgc aggacgcgct gaccgggtcg gtcggtgaag caggcaccgc gctcgacgcc
     2761 gagatccaag cgatcctcga cggtgccgac gcaaccgacg cgaaggcgca ggccgacgaa
     2821 gccgcgcgac aggaactgga cgagttgacc gacgcgttgg atcgtcacat cgccgcgttg
     2881 caggacgacc ttgaggctca gatggcgatc gtcgagtttg cccgcggcgc ggtcgacgca
     2941 cagtacgcgc ttgcccgtgc gcaggacagc tacgcccgca ccctcgagga actgcccggc
     3001 aagctggatg cgatcgcgaa gtcgcaggac tcgaccgcgg agaagcagcg ccgcgtcaac
     3061 gagctctacc gcgctggcgc cgaggacgcc gccggcgtcg ctgacgcgat cgtcgaggcg
     3121 gtcaatcagg cgacgctggc g
//
LOCUS       DAORNF010000377         3140 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_23356_length_3140_cov_1.557536, whole genome shotgun sequence.
ACCESSION   DAORNF010000377 DAORNF010000000
VERSION     DAORNF010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3140
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23356_length_3140_cov_1.557536"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="PLV68_14110"
     CDS             <1..337
                     /locus_tag="PLV68_14110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02434.1"
                     /translation="ASAAEHYQAALRLFRASGDLRGVAYVLAGLGSLSLAQGRARQAA
                     RFFGAVRALRAAVGPFLEAPLQVEHDRDLAAAMCIGEWVGRAATIGAGGELAVAAETT
                     TTTTTTTTA"
     gene            351..>630
                     /locus_tag="PLV68_14115"
     CDS             351..>630
                     /locus_tag="PLV68_14115"
                     /inference="COORDINATES: protein motif:HMM:NF013335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HRE02435.1"
                     /translation="MSDTRIQVLIDCDPGHDDAMALVVAAAYADIVGITTVSGNAPLH
                     LTTRNAIVMTDLLGLDVPVHSGASRPLLAPQIAAESVHGKSGIDGAELP"
     assembly_gap    631..653
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <654..1290
                     /locus_tag="PLV68_14120"
     CDS             <654..1290
                     /locus_tag="PLV68_14120"
                     /inference="COORDINATES: protein motif:HMM:NF013335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HRE02436.1"
                     /translation="GDRAVDFIVDTVRGTEGLWLVPTGPLTNIAMALRAAPDIADRIA
                     GISLMGGGSFGNFTAAAEFNIWCDPEAAAIVYSYGRRLLMCGLDVTHQFQATPERIAA
                     VRAIPGHLATIIADLLVFFSGTYTSRSEHLHGAPIHDACAVLALTHPHLLHSVERHVS
                     VETRGEHTRGMTLIDERWLRERPAPNCTVHTAIDDDQAFAVIAAAIASFSR"
     gene            1306..>2754
                     /locus_tag="PLV68_14125"
     CDS             1306..>2754
                     /locus_tag="PLV68_14125"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HRE02437.1"
                     /translation="MEPLSFPRQSARTQRFTLGEPRSVTVSPDGKRVVFLRSAAGDDP
                     VNCLWLFDTESGDERLIADPRVLLGDDDGDLPPEERARRERAREGAGGVVAYATDTNV
                     TVCSFALAGRLFVAGLISGAARELPVDGPVFDPRPDPTARRVAYVCGATLRIGELDAT
                     SWQLNGEADPDVTWGSADFVAAEEMDRQRGYWWAPDGSAIAVQRTDNSPVQTWHIADP
                     ANPATVPTVHRYPAAGTPNAEVSLHVTSLDGGSVAVEWDRDTYPYLAKVSWPVQDRLL
                     VTVQSRDQRLLTVLAADPVSGATTLLFEDADDAWVELVPGVPALTSGGALVMTADRDG
                     ARRLLVDGAIVTPPELQVRSVVSVADASITFIANDLATPAEQHVWTATLGDTDQGSDG
                     GATCTPITDEPGVHAAITGGGTIVIRSATLAEPGTSTTVHASEPESKPDSEVVHTLVS
                     HAQIPSVVPRVSLRRVGTADLPVAVLLPTTAQS"
     assembly_gap    2755..2764
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2765..>3140
                     /locus_tag="PLV68_14130"
     CDS             <2765..>3140
                     /locus_tag="PLV68_14130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HRE02438.1"
                     /translation="PVLLDPYGGPHAQRVVSSYNAYLSSQWFADQGFAVIITDGRGTP
                     GRGSAWERAVHGDLANPVLDDQVAALHATAAALAEEGVDLDLGRVAIRGWSFGGYLAA
                     LAVLRRPDVFHAAVAGAPVTEWR"
BASE COUNT          447 a         1127 c         1076 g          457 t
ORIGIN      
        1 cgccagcgcc gcggagcatt atcaagcggc cttgcgcctg ttccgggcca gcggcgacct
       61 gcgcggcgtg gcgtatgtgc tggcggggtt gggcagtctg agcctggcgc agggccgcgc
      121 ccggcaagcc gcgcgatttt tcggggccgt gcgggcgctc cgggccgcgg tcggcccgtt
      181 cttggaggcg ccgctgcagg tggagcatga ccgcgacctg gccgccgcga tgtgcatcgg
      241 cgagtgggtc ggcagggccg ccaccatcgg cgccggtggc gaactggcag tagccgccga
      301 gacgacaacg acgaccacga ctacgacgac agcgtgagcg ggcaccggtc atgagcgaca
      361 cccgcatcca agtcttgatc gactgcgacc ccggccacga cgacgcgatg gcgctggtgg
      421 tcgcagccgc ctacgccgac atcgtcggca tcaccaccgt gtcggggaac gcgccgctgc
      481 acctcaccac ccgcaacgcg atcgtgatga ccgatctgct cggcctcgac gtgccggtgc
      541 acagcggcgc ctcccgtccc ctgctcgcgc cgcagatcgc ggccgagagc gtgcatggca
      601 agagcggcat cgacggcgcc gagctgcccc nnnnnnnnnn nnnnnnnnnn nnncggtgac
      661 cgggcggtcg acttcatcgt cgacaccgtg cgcggcaccg aagggctgtg gttggtaccg
      721 accgggccgc tgaccaacat cgcgatggcc ttgcgcgccg cgcccgacat cgccgatcgc
      781 atcgcgggca tctcgctgat gggtggcggc tcgttcggca acttcaccgc ggctgccgag
      841 ttcaacatct ggtgcgaccc cgaggcggcg gcgatcgtgt actcgtacgg gcgccgactg
      901 ttgatgtgcg gcctcgacgt cacccaccag ttccaggcga cgcccgagcg gatcgcagcg
      961 gtgcgagcca tccccggcca tctcgcgacg attatcgccg atctgctggt gttcttcagc
     1021 ggtacctaca ccagccgcag cgagcacctc cacggtgcgc cgatccacga cgcgtgcgcc
     1081 gtgctggccc tgacgcatcc tcacctgctg cacagcgtcg agcgccacgt gtcggtggag
     1141 acgcggggcg agcacacgcg cggaatgacc ctcatcgacg agcggtggct gcgtgagcgc
     1201 cccgcgccga actgcaccgt gcacacagcg atcgacgacg accaggcgtt cgcggtgatc
     1261 gccgcggcga tcgcgtcgtt ctcgcgctga ctacctttgg cggcgatgga accgctctcc
     1321 tttccccgcc agagtgcccg cacgcaacgc ttcacgctgg gcgagccgcg ttcggtcacc
     1381 gtctcacccg acggcaagcg ggtcgtgttc ctgcgcagcg ccgcgggtga cgacccggtc
     1441 aactgcctgt ggttgttcga caccgaatcg ggtgacgaac gcctgatcgc cgacccacgg
     1501 gttctgctgg gcgacgacga cggcgacctg ccgcccgagg aacgggcgcg gcgcgagcgg
     1561 gcccgcgaag gcgcgggtgg tgtcgtcgcc tacgccaccg acaccaacgt cacggtgtgc
     1621 tcgttcgcgc tcgcggggcg gctgttcgtg gccgggctga tcagcggcgc agcgcgcgag
     1681 ttgcccgtcg atggccccgt cttcgacccc cggcccgatc cgacggcccg ccgcgtcgcg
     1741 tacgtctgcg gggcgacgtt gcggatcggt gagctcgatg ccacgtcgtg gcagttgaac
     1801 ggcgaggccg accccgacgt cacgtggggc agcgccgact tcgtcgccgc cgaggagatg
     1861 gatcgccagc gcggctactg gtgggctccc gacggttcgg cgatcgctgt gcagcgcacc
     1921 gacaactcac ccgtgcagac ctggcacatc gccgatccgg ccaacccggc cacggtgccc
     1981 accgtgcacc gctaccccgc cgcgggcacg cccaatgccg aggtttcgtt gcacgtcacc
     2041 tcgttggacg gtggctcggt cgcggtcgaa tgggaccgcg acacctaccc atacctggcc
     2101 aaggtgtcgt ggccggtgca ggaccggctg ttggtcacgg tgcagtcacg cgatcagcgc
     2161 ctgctgacgg tgctggccgc cgatccggtc agcggagcga ccaccctgct gttcgaagac
     2221 gccgacgacg cctgggtcga actggtgccc ggcgtgcccg cgctgacctc cggcggagca
     2281 ctggtgatga ccgccgatcg cgacggcgcg cggcggttgc tcgtcgacgg cgcgatcgtg
     2341 accccgcccg agctgcaggt gcgctcggtg gtctcggtcg ccgacgcatc gatcaccttc
     2401 atcgccaacg acctggctac ccccgccgaa caacacgtgt ggaccgcgac gctcggcgac
     2461 accgatcaag gcagcgatgg cggcgccacc tgcacgccga tcaccgacga gccgggggtg
     2521 cacgccgcga tcaccggtgg cggcacgatc gtgatccgtt ccgccaccct ggccgagccc
     2581 ggcaccagca cgacggtcca cgcaagcgag ccggagagca agccagacag cgaggtcgtc
     2641 cataccttgg tcagccacgc ccagattccc tcggtcgtac cgagggtgtc actgcgccgg
     2701 gtcggtacgg ccgatctccc ggtcgcagta ctgctgccca ccacagccca gtccnnnnnn
     2761 nnnngccggt gctgctcgac ccatacggcg gaccgcacgc gcagcgagtg gtcagctcgt
     2821 acaacgcgta cctgtcgtcg cagtggttcg ccgaccaggg gttcgccgtc atcatcaccg
     2881 acgggcgggg cacgccgggg cgtggcagcg cctgggagcg tgcggtccac ggcgacctgg
     2941 ccaacccggt gctcgacgat caggtcgccg cactgcacgc caccgccgcg gcgctggccg
     3001 aggagggcgt tgacctcgac ctcggccggg tcgccatccg cggctggagt ttcggcggct
     3061 atctcgccgc gctcgccgtg ctgcggcggc ccgacgtgtt ccacgctgcg gtcgccgggg
     3121 caccggttac cgagtggcgg
//
LOCUS       DAORNF010000378         3139 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_23362_length_3139_cov_1.944228, whole genome shotgun sequence.
ACCESSION   DAORNF010000378 DAORNF010000000
VERSION     DAORNF010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3139
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23362_length_3139_cov_1.944228"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>241
                     /locus_tag="PLV68_14135"
     CDS             <1..>241
                     /locus_tag="PLV68_14135"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF024897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HRE02439.1"
                     /translation="LRAFVAAGKPVLGICNGFQVLVRTGLLPGGERRVALGANHDPSG
                     APIPFVCDWVTIAAASRRSIWTAPLVGGDWIECPIA"
     assembly_gap    242..263
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <264..519
                     /locus_tag="PLV68_14140"
     CDS             <264..519
                     /locus_tag="PLV68_14140"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF024897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HRE02440.1"
                     /translation="TCDDATLAALTADDQIAFRYAAGNPNGSRDDIAGICDETGLVLG
                     LMPHPENHVVAHQHPQFSRGHRFGLATALFTAGVAHAASL"
     gene            550..>1245
                     /locus_tag="PLV68_14145"
     CDS             550..>1245
                     /locus_tag="PLV68_14145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HRE02441.1"
                     /translation="MTVTVPVAKLPAPFTDIELPLPDRRVGKVRVSYELPDDERLFVT
                     TDRLSAFDRIVAAVPYKGQVLNELAAWWFANTADIVPNHVRGVPDPNATIGVSARPLP
                     VEVIVRGYITGVTSTSLWKRYADGERVIYGYELPDGLTEHQRLATPLITPTTKAEGGA
                     HDEALTCAEVASSGLVAPALWQRVQDVALALFAKGQQIAADAGLILADTKYEFGLDPD
                     GELIIIDEMHTPDS"
     assembly_gap    1246..1402
                     /estimated_length=157
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1512..2015
                     /locus_tag="PLV68_14150"
     CDS             1512..2015
                     /locus_tag="PLV68_14150"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF012934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034023 - 5-(carboxyamino)imidazole
                     ribonucleotide mutase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HRE02442.1"
                     /translation="MIGHSSPLVVILMGSGADREHCAKIAAVLAELGVDHVQRIGSAH
                     KTPEHVLALLDQYETGPRPVVWVTVAGRSNALSAFVDAKVTSPVIACPPLSLPDDIWS
                     SVRMPAGVGPMLVLDPANAGLAAAKIAALHDTALRQRLADRKAAEAAKVTGADAALHQ
                     ETSVATG"
     gene            2002..>3139
                     /gene="purF"
                     /locus_tag="PLV68_14155"
     CDS             2002..>3139
                     /gene="purF"
                     /locus_tag="PLV68_14155"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HRE02443.1"
                     /translation="MLPVEAMPGADATGHGAPGDDAPHEECGVIGLSTPHGEGVAQMA
                     FFGLFALQHRGQEAAGIAVSDGKLARTHKDAGLVSTVFTPDTLKPLVGYHAIGHTRYS
                     TTGGSSLRNIQPFLVETMHGSLAVAHNGNLVNAATLRDELLNRGFGLSATSDTEVLTL
                     MLAAAGGRTWEERIERTMPAWKGAYSMVILGDDRVIAVRDPWGFRPLSVGRLPKGGWA
                     AASETCALETLGCDDISEVEPGEIVSMVGPDIHRRPAMLPAGSGAHCSFEFVYFSRPD
                     SAWDGRNVHHVRQRLGEELADEWPTDADVVVPVPDSSIPAAIGYSRRSGLPYNDGLIK
                     NRYIGRTFIEPTQAMRDRGVALKFNALGANLEGKRVVLIDDSLVR"
BASE COUNT          444 a         1086 c          975 g          455 t
ORIGIN      
        1 ctgcgcgcct tcgtcgccgc gggcaagccg gtgctcggca tctgcaacgg cttccaggtg
       61 ctggtgcgca ccggtctgct ccccggcggt gagcgccgcg tagcactcgg cgccaaccac
      121 gacccgtccg gcgctccgat cccgttcgtc tgcgactggg tcaccatcgc cgccgcgtca
      181 cgccgcagca tctggaccgc accgctggtc ggtggcgact ggatcgagtg cccgatcgcc
      241 cnnnnnnnnn nnnnnnnnnn nnncacgtgt gacgacgcga cgctcgccgc gctgaccgcc
      301 gacgatcaga tcgcgttccg gtacgcggcg ggcaacccca acggatcgcg tgacgacatc
      361 gccggcatct gcgacgagac cggcctcgtg ctgggcctga tgcctcaccc cgagaaccac
      421 gtcgtcgccc atcagcaccc ccagttcagt cgtggccacc gcttcggtct cgccacggcc
      481 ctcttcaccg ccggcgtcgc ccacgccgcc tcgctctgac ctgactgctc tgatcatcgg
      541 aggatcccca tgaccgtcac cgttccagtc gccaagttgc ccgccccgtt caccgacatc
      601 gagctcccgc tgcccgaccg ccgggtcggc aaggtgcggg tgagctacga gctgcccgac
      661 gacgagcggc tgttcgtcac caccgaccgg ctctcggcct tcgaccgcat cgtcgccgcc
      721 gtgccgtaca agggtcaggt gctcaacgag cttgctgcgt ggtggttcgc caacaccgcc
      781 gacatcgtgc ccaaccacgt ccgcggcgtg cccgacccga acgcgacgat cggcgtgtcg
      841 gctcgcccgc tgccggtcga ggtgatcgtg cgtggctaca tcacgggggt gacctcgacg
      901 tcgctgtgga agcgctacgc cgacggcgaa cgggtgatct acggctacga actgcccgac
      961 gggttgaccg agcaccagcg tctcgccacg ccgctgatca cgccgaccac caaggccgag
     1021 ggcggtgccc acgacgaggc gctgacctgt gccgaggtcg ccagcagcgg cctggtggca
     1081 ccggcgctgt ggcagcgagt gcaggacgtc gccttggccc tgttcgccaa ggggcagcag
     1141 atcgctgccg atgccgggct gatcctggcc gacaccaagt acgagttcgg tctcgatccc
     1201 gacggcgagc tgatcatcat cgacgagatg cacacgcccg actcgnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nncggcccgc tacgtcaacg ccttcgagcg actgacgtgc
     1441 cgccccttca tccccggcct ctaccccgtc gccccccgcc tgaccgacgc cctccacaag
     1501 gtcggtgcgc tgtgatcgga cactcctccc cgctcgtggt cattctgatg gggtcggggg
     1561 cggatcgcga gcattgcgcc aagatcgccg cggtgttggc cgaactgggc gtcgaccacg
     1621 tgcagcgcat cgggtcggcg cacaagacgc ccgagcacgt gctcgcactg ctcgaccagt
     1681 acgagaccgg tccccgcccg gtggtgtggg tcacggtggc cgggcgcagc aacgcgctgt
     1741 cggccttcgt cgacgccaag gtcacctcgc cggtcatcgc ctgccccccg ctgtcgctgc
     1801 ccgacgacat ctggtccagc gtgcgcatgc ccgccggggt cggcccgatg ctggtgctcg
     1861 acccggccaa cgccggcttg gccgcggcca agatcgctgc cctgcacgac actgcgctgc
     1921 gtcaacgcct ggccgatcgc aaggctgccg aagcggccaa ggtcaccggc gccgatgcgg
     1981 ccctgcacca ggagacgtca gttgctaccg gttgaggcaa tgccgggtgc cgacgcgacc
     2041 ggtcatggtg cgccgggtga cgatgcaccg cacgaggagt gcggtgtcat cggcctctcc
     2101 acgccccacg gcgagggcgt ggcccagatg gcgttcttcg gattgttcgc gttgcagcat
     2161 cgcggccagg aagccgccgg catcgcggtc agcgatggca agctggcccg cacccacaag
     2221 gacgctggct tggtcagcac cgtgttcacc ccggacaccc tcaagccgct ggtcggctac
     2281 cacgccatcg gccacacccg ctacagcacc accggtggct cgtcgctgcg caacatccag
     2341 ccgttcctgg tcgaaacgat gcacggcagc cttgccgtcg cccacaacgg caatctggtc
     2401 aacgcggcaa cgctccgcga cgagttgctc aaccgcggct tcggcctcag cgccaccagc
     2461 gacaccgagg tgctcacgct gatgctcgcc gcggctggtg gccgcacgtg ggaagagcgg
     2521 atcgagcgga cgatgcccgc ctggaagggt gcgtactcga tggtgatcct cggcgacgac
     2581 cgggtgatcg cggtgcgcga cccgtggggc ttccggccgc tgtcggtcgg gcgcctgccc
     2641 aaaggcgggt gggccgcggc atcggagacg tgcgccctgg aaacgttggg gtgtgacgac
     2701 atcagtgagg tcgagccggg cgaaatcgtc agcatggtcg ggcccgacat ccaccgccgt
     2761 ccggcgatgc tgcccgccgg tagcggggcg cactgctcgt tcgagttcgt ctacttcagc
     2821 cgtcccgaca gcgcctggga tggccgcaac gtgcaccacg tgcgccagcg cctgggcgag
     2881 gagctggccg acgagtggcc caccgatgcc gacgtggtcg tgccggtgcc cgactcgtcg
     2941 atcccggctg cgatcgggta ttcgcgccgc agcgggctgc cctacaacga cggcctgatc
     3001 aagaaccgct acatcggtcg caccttcatc gagccgacgc aagcgatgcg cgaccgtggc
     3061 gtggcactga agttcaacgc gctcggcgcc aacctcgagg gcaagcgggt ggtgctgatc
     3121 gacgactcgc tcgtgcgcg
//
LOCUS       DAORNF010000379         3138 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_23382_length_3138_cov_1.383717, whole genome shotgun sequence.
ACCESSION   DAORNF010000379 DAORNF010000000
VERSION     DAORNF010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3138
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23382_length_3138_cov_1.383717"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            17..>249
                     /locus_tag="PLV68_14160"
     CDS             17..>249
                     /locus_tag="PLV68_14160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02444.1"
                     /translation="MTAVPSPWLNEWFHASLEQVRNAFPVRVLEEDLKRQPSEILLRG
                     GGALAQDEQDTLRLVEPTIEPAPSTRASGGSLGL"
     assembly_gap    250..401
                     /estimated_length=152
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <402..821
                     /locus_tag="PLV68_14165"
     CDS             <402..821
                     /locus_tag="PLV68_14165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HRE02445.1"
                     /translation="CSPGDRVAADRAAMMPLPPVPPEVGWRKTLRLPRDHYVRLDSND
                     YSVHPAVVGRRIEIHADLDRVWVTCEGAIVADHARVWAQHQTITDFEHTVAAKLLRQG
                     RGDLLRPVADAATGEEVEIRSLGVYDQVLGLVDEEVC"
     assembly_gap    941..976
                     /estimated_length=36
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <977..1712
                     /gene="istB"
                     /locus_tag="PLV68_14170"
     CDS             <977..1712
                     /gene="istB"
                     /locus_tag="PLV68_14170"
                     /inference="COORDINATES: protein motif:HMM:NF038214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HRE02446.1"
                     /translation="FLAACLQREVSARESHGGEGRIRAARFPARKSLEDFDYDHARGL
                     KRETIAHLGTLDFVAAKENVVLLGPPGTGKTHLATGLAIRACQAGHRVQFATASQWVD
                     RLAEGHHAGRLQDELVRYPVLVIDEVGYIPFEPEAANLFFQLVSSRHERASLIVTSNK
                     NFARWGEVFGDDTVAAAMIDRLVHHAEVIALKGRLLPAEEPRTRPRPRGRYRREPVAE
                     GVSFRLPRGGQISAAVDMDAGVHDMW"
     assembly_gap    1744..1836
                     /estimated_length=93
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1837..>2699)
                     /locus_tag="PLV68_14175"
     CDS             complement(<1837..>2699)
                     /locus_tag="PLV68_14175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013160159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HRE02447.1"
                     /translation="LTVARVAAGLGVAWDTANNAVLAEGKRLLINDPTRFEGVKVIGV
                     DEHVWRHTRRGDKYVTVIIDLTPVRDGAGPARLLDMVEGRSKAAFKTWLADRPQAWRD
                     GIEVVAMDGFTGFKTAATEELPDVVTVMDPFHVTRLAGEALDECRRRVQQAIHGHRGM
                     TGDPLYAARRTLCTGADLLTDRQVDRLTTLFADDAHVEVEATWGIYQRMIAAYRHPDR
                     RRGRELMVNLIDSISAGVPTALVEITKLGRTLTKRAQDVLAYFDRPGTSNGPTEAING
                     RLEHLRGSALG"
     assembly_gap    2700..2791
                     /estimated_length=92
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2792..3087)
                     /locus_tag="PLV68_14180"
     CDS             complement(<2792..3087)
                     /locus_tag="PLV68_14180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HRE02448.1"
                     /translation="MSDATPPAGFGRPDLTAFARLDGLGLSVTGQRLEPDRAVLACRV
                     VEPDQWCRRCGSEGAARDTVIRRLAHEPLGWRPTVLEVVVRRYRCADCGHVWRQ"
BASE COUNT          437 a          941 c          935 g          452 t
ORIGIN      
        1 cgtacgacat gggaaggtga cggccgttcc atctccttgg ctcaacgagt ggttccacgc
       61 cagcctcgag caggtccgca acgccttccc ggtccgcgtg cttgaggagg acctgaagcg
      121 ccagcccagc gagatccttc tgcgcggtgg tggagctctc gcgcaggacg aacaggacac
      181 gctcagactc gtggagccca ccatcgaacc tgcgccctcc acccgtgcct ccggtgggtc
      241 gttgggtctn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntgcagcccg ggtgatcggg
      421 tcgcagccga ccgggcagcg atgatgccgc taccgcctgt tccgcccgaa gtgggctggc
      481 gcaagaccct gcggctgccg cgcgaccact acgtgcgact cgattccaac gactactccg
      541 tccaccccgc tgtggtcggt cggcggatcg agatccacgc cgacctggac cgggtgtggg
      601 tgacgtgtga gggagcgatc gtggccgacc acgcccgcgt gtgggcgcag catcagacga
      661 tcaccgactt cgaacacacc gtcgccgcca agctcctgcg ccagggacgt ggcgatctgc
      721 tgcggccggt tgcggacgcg gccaccggcg aggaggtcga gatccgatca ctcggcgtct
      781 acgaccaggt cctcggactc gtcgatgagg aggtctgctg atggccaccc gcaccaccac
      841 caaggccgtc tcgaagacgg gacgggacgt caccagcgag ttggagtacc tgacccgggc
      901 gttgaaggcg ccgacactgc gcgagtcggt cgagcggctc nnnnnnnnnn nnnnnnnnnn
      961 nnnnnnnnnn nnnnnngttc ctggccgcct gcctgcagcg tgaggtgtca gcacgcgagt
     1021 cccacggtgg tgagggccgg atccgtgccg cccggttccc cgcccgcaag tcgctggagg
     1081 acttcgacta cgaccacgcc cgcggcctga agcgggagac catcgcccac ctgggcaccc
     1141 tggacttcgt cgccgccaag gagaacgtcg tcttgctcgg gccaccggga accggcaaga
     1201 cacatctggc cactggcctg gcgatccggg cctgtcaggc cggccaccgg gtccagttcg
     1261 ccaccgcgtc ccagtgggtc gaccggctgg ccgagggcca ccacgccggg cggctgcaag
     1321 acgagctggt gcgttacccg gtactggtca tcgacgaggt cggctacatc cccttcgaac
     1381 ccgaggccgc caacctgttc ttccagctcg tctcatcgcg ccacgagcgc gccagcctga
     1441 tcgtcacgtc caacaagaac ttcgcccgct ggggcgaggt cttcggcgac gacaccgtcg
     1501 cggccgcgat gatcgaccgg ctcgtccacc acgccgaggt catcgccctg aaagggcgac
     1561 tcctaccggc tgaagaaccg agaactcggc cgcgtccccg tggccgttac cgaagagaac
     1621 cagtagccga gggggtcagt tttcgattgc cgcgaggggg tcagatttca gccgccgttg
     1681 acatggacgc cggcgtgcat gacatgtggt gactgcgttc tgaaccgagt cctccagcta
     1741 gccnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnaacc cgagtgcgga gccgcggagg
     1861 tgttcgagcc gtccgttgat ggcctcggtc ggcccgttgg acgtgccggg ccggtcgaag
     1921 taggcgagca cgtcttgggc acgcttggtc agggtccggc ccagcttggt gatctcaacc
     1981 agtgctgttg ggacgccggc gctgatcgag tcgatgaggt tgaccatcag ctcgcggcca
     2041 cggcgccggt cggggtgtcg gtaggcggcg atcatccgct ggtagatgcc ccaggtcgcc
     2101 tcgacctcga catgggcgtc gtcggcgaac aaggtggtga gccggtcgac ctgcctgtcg
     2161 gtgagcaggt cggcaccggt gcacagcgtc cttcgtgcgg catacagcgg gtcgccggtc
     2221 atcccgcggt ggccatggat ggcttgttgg acccggcgcc ggcactcatc gagcgcctcg
     2281 cctgccagcc tggtgacgtg gaagggatcc atcacggtca ccacatcagg cagctcctcg
     2341 gtggcggcgg tcttgaagcc ggtgaacccg tccatcgcga ccacctcgat tccgtcgcgc
     2401 cacgcctgtg ggcggtcggc gagccaggtc ttgaacgccg ccttcgaccg gccctcgacc
     2461 atgtccagca gccttgctgg gccggcgcca tcgcggaccg gggtgaggtc gatgatcacg
     2521 gtgacgtact tgtcgccacg cctggtgtgg cgccagacgt gctcatcgac gccaatgacc
     2581 ttcacgccct caaaccgcgt ggggtcgttg atcagcagcc gcttgccttc agccaggacc
     2641 gcgttgttgg cggtgtccca cgcgaccccg agtcccgcgg cgacacgtgc gacggtgagn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntcttggcgc cacacgtgtc cgcagtcggc
     2821 acagcggtag cggcgcacta caacttccag cacggtcggt cgccagccca gcggctcgtg
     2881 ggccaaccgc cggatcacgg tgtcacgagc agcgccttcg ctgccgcacc gtcggcacca
     2941 ctgatctggt tccaccacgc ggcacgcgag gaccgcacga tccggttcaa gtcgttgtcc
     3001 ggtcacgctc agaccgaggc cgtcgagtcg agcgaaggcg gtcaggtcag ggcggccgaa
     3061 gccggccggc ggggtagcgt cggacacgtc gaggtctttc ggatggatgg cgtaggaacc
     3121 tccgtcgtcg ggagacct
//
LOCUS       DAORNF010000380         3136 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_23409_length_3136_cov_1.779617, whole genome shotgun sequence.
ACCESSION   DAORNF010000380 DAORNF010000000
VERSION     DAORNF010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3136
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23409_length_3136_cov_1.779617"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(24..398)
                     /locus_tag="PLV68_14185"
     CDS             complement(24..398)
                     /locus_tag="PLV68_14185"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HRE02449.1"
                     /translation="MTYKVRMSVVAIATAACIVAACGSGDGDAGSTLPPVQSTTTTTT
                     LPPTTTTRPDTYRIQSGDTLFKIAQRFGLTTEELAAYNNITNFDHVEKDAVLRIPLPG
                     QVVVTTTTTPPPPGDSVPPSSS"
     gene            602..>791
                     /locus_tag="PLV68_14190"
     CDS             602..>791
                     /locus_tag="PLV68_14190"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRE02450.1"
                     /translation="MTPNQATDNEQLTAGPLSGVRVLEMGSFIAGPFAGQLLGDYGAD
                     VIKIEPPGDGDPMRRWGIT"
     assembly_gap    792..805
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <806..>1229
                     /locus_tag="PLV68_14195"
     CDS             <806..>1229
                     /locus_tag="PLV68_14195"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRE02451.1"
                     /translation="LWWPTIARNKRSVAVDLRTAEGQAVVRRLALGCDIVLENFRPGR
                     LAEWGLDYASLAARNPRIVMVHVSGFGQTGPYATQAGFGSVGEAMGGIRHTTGNPGQP
                     PSRAGISLGDALAALFAVNGAATAYAAALRTGRGQEVDV"
     assembly_gap    1230..1244
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1245..1840
                     /locus_tag="PLV68_14200"
     CDS             <1245..1840
                     /locus_tag="PLV68_14200"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRE02452.1"
                     /translation="EAVAALMESTLADHEIAGVTRSRTGSVLPGVAPSNVYPCADGSE
                     VIIAANADTVFRRLCAAMGTPALADDQRFAEHHARGVNMVELDGIIGAWTARFDGTAL
                     LEHLAANGIPAGRIYTAPDMLTDPQYLAREMVLRTRTPEGWDLPMTGIVPKFTATPGR
                     VRFTGPTLGEHTRAVLAEIGGFSAAEVDSLYAAGTVA"
     gene            complement(1872..2930)
                     /locus_tag="PLV68_14205"
     CDS             complement(1872..2930)
                     /locus_tag="PLV68_14205"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HRE02453.1"
                     /translation="MIPVAPPTLAGIASRVEERLRSLIDAEQAHWQGLNEVLSDPFDA
                     IRTLVLAGGKRLRPAFCHWGFVGAGGDPDDPAVIDAGAALELLHAFALFHDDVMDDSD
                     TRRGEPTVHTLHAQRHGGRWAGDARRYGEGVAILVGDLTFVYADQLMRSAPLPAWDIW
                     NELRIELNIGQYLDIVGSAMRERRWQRAEQICRYKSGKYTIERPLQLGVAMALPSQVG
                     GMLGALSAYGLPLGDAFQMRDDVLGVFGDTAITGKPVADDLREGKPTPLLALATEAAD
                     ASQTALLDRVGAPDLSDDEIAAIQQVIVSTGALDRLEDHITQLTAAAVAALDAMPLTP
                     GARDELRLLASYVSWRDI"
BASE COUNT          494 a         1087 c         1067 g          459 t
ORIGIN      
        1 caccgtcgcc ggacagtcca acgtcaggac gaggagggtg ggacggagtc gcctggcggg
       61 ggtggagtgg tggtcgtggt cacgacgacc tggccgggga gggggatgcg cagcacggcg
      121 tccttttcca cgtggtcgaa gttggtgatg ttgttgtagg cggccagttc ctcggtggtc
      181 agcccgaacc gctgggcgat cttgaacagc gtgtcgcccg actggatgcg gtaggtatcc
      241 ggccgggtcg tggtcgtcgg cggcagcgtg gtggtggtcg tggtcgactg cacgggcggc
      301 agcgtgctgc ctgcatcgcc atcgcccgag ccgcaggcgg ccacgatgca tgcggccgtg
      361 gcgatcgcca cgacactcat ccgaaccttg tacgtcattg cctcaccctt gacgtctcgt
      421 cggccggaca cactcgtccg gccctcggtg cgcaaggcta ggcaacggct cactccctgt
      481 ggccgcgacc cccgaaagcg acacgcgagt gcaacgtgac ggaagcaatc gacaccatga
      541 cacccaagaa ccatgacacc caagaaccat gacacctgag caacaccaca cctgagcaac
      601 catgacaccg aaccaagcga cagacaatga acaactgacg gcggggccgc tgagcggcgt
      661 gcgcgtgctg gagatgggca gcttcatcgc cgggcccttc gccggccagc tgctgggcga
      721 ctacggcgcc gacgtgatca agatcgagcc gccgggcgac ggcgacccga tgcggcgctg
      781 gggcatcacc gnnnnnnnnn nnnnncctgt ggtggccgac gatcgcgcgt aacaaacggt
      841 cggtcgcggt cgatctgcgc accgccgaag ggcaagccgt cgtgcgccgc ctggcgttgg
      901 gctgcgacat cgtgctggag aacttccgtc ccggtcgctt ggccgagtgg gggctcgact
      961 acgcctcgct ggccgcacgc aacccacgga tcgtgatggt gcacgtgagc gggttcggcc
     1021 agaccgggcc gtacgccacc caagccggct tcggcagcgt gggtgaagcg atgggtggca
     1081 ttcgccacac caccggcaac cccggccaac caccatcgcg ggccggcatc agcctgggcg
     1141 acgcactcgc cgcgctgttc gccgtcaacg gggcggccac cgcctatgcc gcggcgttgc
     1201 gcaccggacg cggccaagag gtcgacgtcn nnnnnnnnnn nnnnacgagg cggtcgcggc
     1261 cctgatggag tcgaccctcg ccgaccacga gatcgccggg gtcacccgca gccgcaccgg
     1321 cagtgtgctg cccggcgtgg caccgtcgaa cgtgtacccg tgcgccgacg gcagcgaggt
     1381 gatcatcgcc gccaacgccg acaccgtgtt ccgccgcctg tgcgcagcga tgggaacgcc
     1441 tgcgctcgcc gacgaccagc gcttcgccga gcaccacgct cgcggcgtca acatggtcga
     1501 gctcgacggc atcatcggcg cctggaccgc tcgcttcgac ggcaccgcgc tgctcgaaca
     1561 tctcgccgcc aacggcatcc ctgccggacg tatctacacc gcacccgaca tgctgaccga
     1621 cccgcagtac ctggcccgcg agatggtgct gcgcacccgc acccccgaag ggtgggacct
     1681 accgatgacc ggcatcgtcc ccaagttcac cgccactccc gggcgggtcc gcttcacggg
     1741 tcccacgctc ggcgagcaca cccgcgccgt gctggccgag atcggtggct tcagcgcggc
     1801 cgaggtcgac tcgctctacg cggccggcac cgtcgcctga ggggttggta cgtgagctcg
     1861 cccgccggag ctcagatatc gcgccagctc acgtagctgg ccaacaggcg cagctcgtcg
     1921 cgtgcgcccg gggtgagcgg catcgcatcg agggcggcga ccgccgccgc ggtcagctgg
     1981 gtgatgtggt cttccaagcg gtcgagcgcg ccagtggaca cgatgacctg ctggatggcc
     2041 gcgatctcgt cgtcgctcag atcgggcgcg cccacccggt cgagcagggc ggtctggctg
     2101 gcgtcggccg cttccgtcgc cagcgcgagc agcggggtgg gcttgccttc gcgcaggtcg
     2161 tcggccacgg gcttgcccgt gatcgcggtg tcgccgaaca ccccgagcac gtcgtcgcgc
     2221 atctggaacg catcgcccag tggcagacca taggccgaga gcgccccgag catgccgccg
     2281 acctggctgg gaagggccat cgccacgccg agttgcaggg gccgctcgat cgtgtacttg
     2341 ccgctcttgt agcggcagat ctgctcggcc cgctgccacc gccgctcgcg catcgccgag
     2401 ccgacgatgt cgaggtactg gccgatgttg agctcgatcc gcagctcgtt ccaaatgtcc
     2461 cacgcaggca acggcgccga gcgcatcaac tggtcggcgt agacgaaggt caggtcgccg
     2521 accaggatcg cgacaccctc gccgtagcgg cgggcgtcac ccgcccaacg gccgccgtga
     2581 cgctgggcgt gcagggtgtg cacggtcggc tcgccgcggc gggtgtcgga gtcgtccatc
     2641 acgtcgtcgt ggaacagcgc gaacgcgtgc agcagttcca acgcggcacc tgcgtcgatc
     2701 acggccggat cgtctggatc gccaccggcg ccgacgaagc cccagtggca gaacgcggga
     2761 cgcaagcgct tgcccccggc cagcaccagt gtgcggatcg cgtcgaacgg gtcgctgagc
     2821 acctcgttga ggccttgcca gtgcgcttgc tcggcatcga tcagcgaccg cagccgctct
     2881 tcgacccgtg atgcgatacc agccaacgtc ggtggggcga cggggatcac gccaacgagg
     2941 ctaacgccac ccgcgccacg gcccgcgatc gccgatcgac tcaccgacag gatcggtcgt
     3001 tcagtcgtcg ttgagctcgg cgacgacggt ttcgatctgc atccgcgcgt tgccgatgcg
     3061 ctggcgacag acccggatca gttcggccgc gcggtggacg tggctggcca agcggtcgac
     3121 atcgacgttg tcgcgt
//
LOCUS       DAORNF010000381         3128 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_23471_length_3128_cov_1.907257, whole genome shotgun sequence.
ACCESSION   DAORNF010000381 DAORNF010000000
VERSION     DAORNF010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23471_length_3128_cov_1.907257"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /locus_tag="PLV68_14210"
     CDS             <1..660
                     /locus_tag="PLV68_14210"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HRE02454.1"
                     /translation="MDEARRAKILAAETATAEGRFENEIIAVPYDDGEGEKLLTADEG
                     IRPGTTAESLAKLKPAFKEDGKITAGNSSQITDGASAVLIMSADKAAELGLKPRARFH
                     TFAVAGADPVTMLTGPIPATKKALEKSGLTIDQIDLFEVNEAFASVVLAWAKEVGADL
                     DKVNVNGGAIALGHPLGCSGTKLMATLLNELERSGGRYGLQVMCEGGGMANATIIERI
                     R"
     assembly_gap    674..694
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(803..1009)
                     /locus_tag="PLV68_14215"
     CDS             complement(803..1009)
                     /locus_tag="PLV68_14215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HRE02455.1"
                     /translation="MTTTTYTVTGMTCGHCAAAVSEEIGKLATVRTVDVDLPTGAVTV
                     ESDAPLSRADVAAAVDEAGYELAQ"
     gene            1074..1418
                     /locus_tag="PLV68_14220"
     CDS             1074..1418
                     /locus_tag="PLV68_14220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HRE02456.1"
                     /translation="MTSAACDQPGQHPGGHPGDNAERGYSMDKSAYLTRLKRIEGQVR
                     GLQRMIDEDVYCIDVLTQVSAVTKALQSVAVGLLDQHVRHCVSHAITSDPDHADEKIT
                     EATRAIERLIKS"
     gene            complement(1432..>3128)
                     /locus_tag="PLV68_14225"
     CDS             complement(1432..>3128)
                     /locus_tag="PLV68_14225"
                     /inference="COORDINATES: protein motif:HMM:NF008453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRE02457.1"
                     /translation="PAPIVEVSHLNVDFWVDGTWYPAVIDANFELRSGEVLAIVGESG
                     SGKSTAALSLMGLLPKNASVRGSIKLDGRELVGIDQRTLRSLRGREVSVIFQEPMTAL
                     NPVYTVGFQIAEMLRSHQAMAPKAARKRAIELLKMVEIPEPERRVDAYPHQLSGGQRQ
                     RAMIAQALAMEPRVLIADEPTTALDVTVQAEILKLLRDLRDRIDAGVLLITHDMGVVA
                     DLSDRVIVMEKGYIVEAGSADHVFHRPEHPYTQRLMQAVPHLGSIVTAGPEAETVVPA
                     NASDAIADAPNDVIVDLRDVAIEYPRQGTTPAFRAVDGVSMSIGAAEVVGLVGESGSG
                     KTTIGRAIVGLLPFVEGTASVLGTNMVGVSKEALRHTRSQLSFVFQDPGSSLNPRLPI
                     GESIGEPLLLHKVAKGAALDRRVEELLDQVQLARSLRNRYPHELSGGQRQRVGIARAL
                     ALNPKLLIADEPTSALDVSVQAKVLDLFQELQRDIGFACLFISHDLAVVEILASRIAV
                     MQHGKLVEFGTREQILTDPQEMYTKRLLAAVPVPDPDEQRERRIERDALIAATEAS"
BASE COUNT          557 a         1062 c         1004 g          484 t
ORIGIN      
        1 atggacgagg cgcgcagggc gaagatcctt gccgccgaga ccgccaccgc cgagggtcgc
       61 ttcgagaacg agatcatcgc cgtgccgtac gacgacggtg agggcgagaa gttgctgacc
      121 gccgacgagg gcatccgccc cggcaccacc gccgagtcgc tggccaaatt gaagcccgcc
      181 ttcaaggaag acggcaagat cacggcgggc aactccagcc agatcacgga cggcgcgtcg
      241 gccgtgttga tcatgagcgc cgacaaggca gccgagctcg gcctcaagcc ccgcgcccgg
      301 ttccacacct tcgccgtggc cggtgccgac cccgtcacga tgctgaccgg cccgatcccg
      361 gccaccaaga aggcgctcga gaagagtggc ctgaccatcg accagatcga cctgttcgag
      421 gtcaacgagg ccttcgcctc cgtcgtgctc gcctgggcca aggaggtcgg cgccgatctc
      481 gacaaggtca acgtcaacgg cggcgcgatc gcgctcggcc acccgctcgg ctgcagcggc
      541 accaagctca tggcgacgct gctcaacgag ttggagcgca gcggcggccg ctacggcctg
      601 caggtgatgt gcgagggcgg cgggatggcc aacgccacga tcatcgagcg gatccgctga
      661 tcgccatgcc tcgnnnnnnn nnnnnnnnnn nnnngcggcg gatggtgacc gaccgggtgc
      721 gacatgctcg cacccggtcg gatgcttttc ggcacgcgcc gcgtgccggt catgacaccc
      781 gtcggcacgc gccgcgtgcc ggtcactgcg ccagttcgta cccagcttcg tcgaccgcag
      841 cggcgacgtc ggcgcgggaa aggggtgcat cggattcgac ggtgaccgcg ccggtcggca
      901 gatcgacatc gaccgtgcga actgtggcca acttaccgat ctcttcggac acggccgcag
      961 cgcagtggcc gcaggtcatg ccggtaacgg tgtaggtggt ggtcgtcatg ggggctcctt
     1021 gcgacatcga ctgattgata ccccaccagg gtatcctcgc tacactgttc tgcgtgacct
     1081 cggcggcgtg tgatcaaccc ggccagcatc caggcggcca tcccggcgac aacgccgagc
     1141 gcgggtactc gatggacaag tcggcctacc tcacccgcct caagcggatc gaaggccagg
     1201 tgcgtggtct ccagcgaatg atcgacgaag acgtctattg catcgacgtg ctcacccagg
     1261 tcagcgccgt gaccaaggcg ctccaaagcg ttgccgtcgg cctgctcgac caacacgtcc
     1321 gtcactgcgt cagccacgcg atcacgtccg accccgatca cgccgacgag aagatcaccg
     1381 aagccacacg agccatcgaa cgtctcatca agtcctgagc cgaactggtc gttacgaagc
     1441 ctctgtcgcg gcgatcaggg cgtcccgttc gatgcgccgc tcgcgctgct cgtcggggtc
     1501 gggcaccggc accgcggcca gcaggcgctt ggtgtacatc tcctgcgggt cggtcaaaat
     1561 ctgctcacgc gtgccgaact cgacgagctt gccgtgctgc atcacggcga tgcggctggc
     1621 gaggatctcg accacagcga ggtcgtggga gatgaacagg caggcaaagc cgatgtcgcg
     1681 ctgcagctct tggaacaggt cgagcacctt ggcctgcacc gacacgtcga gcgcagaggt
     1741 cggctcgtcg gcgatcaaca gtttcgggtt gagcgcgagt gcacgggcga tgccgacacg
     1801 ctgacgctga ccgcccgaca gttcgtgcgg atagcggttg cgcagcgaac gggcgagctg
     1861 cacctgatcg agcaactcct cgactcggcg gtcgagtgcg gctcccttgg ccaccttgtg
     1921 cagcagcagt ggttcgccga tcgactcgcc gatcggcagc cgcgggttga gcgacgaacc
     1981 cggatcttgg aacacgaagc tcaactgact gcgggtgtgg cgcagcgcct ctttcgacac
     2041 gccgaccatg ttcgtcccca gcaccgacgc ggtgccttcg acgaagggca gcaggccgac
     2101 gatcgcccgg ccgatggtgg tcttgccaga gccagactcg ccaaccaggc cgacgacctc
     2161 ggccgcgccg atgctcatcg acacaccgtc gaccgcccgg aacgcagggg tcgtgccctg
     2221 acgcggatac tcgatcgcca catcgcgcag gtcgacgatg acgtcgttcg gtgcatccgc
     2281 gatcgcgtcg gaggcattgg ccggcacaac ggtctcggcc tcgggcccgg ccgtgacgat
     2341 cgagccgagg tgcggcacag cctgcatcaa ccgctgcgtg tagggatgct cgggacggtg
     2401 gaacacgtgg tcggccgaac cagcttcgac gatgtaaccc ttctccatca cgatcacccg
     2461 atcggacaga tcggcgacga cccccatgtc gtgggtgatc agcagcacgc cagcatcgat
     2521 gcggtcgcgc aggtcgcgca gcagcttcaa gatctcggcc tgcaccgtga cgtcgagcgc
     2581 ggtggtcggc tcgtccgcga tcaacacccg cggctccatc gccaacgcct gggcgatcat
     2641 cgcccgctgc cgctgaccac ccgagagctg gtgcgggtac gcgtcgacgc gccgctcggg
     2701 ctcgggaatc tcgaccatct tcagcagttc gatcgcccgc ttgcgggcgg ccttgggcgc
     2761 catcgcctgg tgactacgca gcatctcggc gatctggaat cccaccgtgt acaccgggtt
     2821 gagcgcggtc atcggctctt ggaagatgac cgacacctca cggccacgca acgaacgcag
     2881 tgtgcgctga tcgatgccga ccagctcgcg tccgtcgagc ttgatcgaac cgcgcacgct
     2941 ggcgttcttg ggcagcagac ccatcaacga cagcgccgcg gtcgacttac ccgaccccga
     3001 ctcgcccacg atggccagca cttcccccga acggagttcg aagttggcat cgatgaccgc
     3061 cggataccac gtgccgtcga cccagaagtc gacgttgagg tgcgacacct ccacaattgg
     3121 ggctggca
//
LOCUS       DAORNF010000382         3119 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_23575_length_3119_cov_1.589099, whole genome shotgun sequence.
ACCESSION   DAORNF010000382 DAORNF010000000
VERSION     DAORNF010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3119
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23575_length_3119_cov_1.589099"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>629
                     /locus_tag="PLV68_14230"
     CDS             <1..>629
                     /locus_tag="PLV68_14230"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRE02458.1"
                     /translation="GDPFPDFIDPALTTGIEVVGSIQTPNLEKIAALNPDLIIGTQLV
                     VAPLQDELDTIAPTVATHYAFYVSNWRDDVLLAADAGGVLDQVRAKLSEFDARVETLH
                     AELTADGDGPTLSRVDVFSGSLVYYRYACSAFGEVLLSVGIRQPEAQAGDCTDNDYTS
                     VLQYPSLEQVDVFDADVIVAYQHQAGSTDVGANPLDVISTSPLWGNLAA"
     assembly_gap    630..639
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1016..>1804
                     /locus_tag="PLV68_14235"
     CDS             1016..>1804
                     /locus_tag="PLV68_14235"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRE02459.1"
                     /translation="MVQHVLLAASTWMSHRAAFATLEQLRLRIGDRLGRVPLGFITSR
                     RSGEIQRTMNDDVERLELFLAHAIPDIVSGVVVVVATTAWMFAVDWRMALATVAIVVI
                     SVPMMSAGMKKGEAKLGMYMASLARMNGSIVEFVRGLPVVRTFNRSDETFAETRSAIE
                     DSADFQSQWGREFLPLFTTFYTLLASNIVTIVPIGLWLWTSDRLSTSDLLFFFIIGFG
                     YPLPLLKLMEFTSQMSYLSLSANLVDELDSARELPEAPQRAELAG"
     assembly_gap    1805..2002
                     /estimated_length=198
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2003..2621
                     /locus_tag="PLV68_14240"
     CDS             <2003..2621
                     /locus_tag="PLV68_14240"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRE02460.1"
                     /translation="IREMPFEQLMEQVAFVFQETFLFDDTIAANLRLGRPDATDAEVE
                     AVARAARAHDFIVAKPDGYATRLGERGAQLSGGERQRLSIARAMLKNAPIIVLDEATA
                     FADPENEAAIQDALNALAVDKTVVVIAHRLSTIVGADEIVVLDAPAGEPGRVVERGHH
                     ADLVAAGGLYSRMWAAFEDVEGIELGRAVHAATEPAASIAGEVGP"
     gene            2618..>3119
                     /locus_tag="PLV68_14245"
     CDS             2618..>3119
                     /locus_tag="PLV68_14245"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HRE02461.1"
                     /translation="MSITITNNGTNHGTTDRFYAGTIRTVIRLFGDQRRVLYVGIGLR
                     MLNAVCIGVGAGVTVAVIRRLGDSGADRLDGGDVWTVTAVLVASVIGQYVTGYFSNRF
                     AWVSTFQAIGQARLRVLRHLQRVPLGSVLSRRSGDITAVLTSDLEMVSNFAHHAMPAL
                     FGAVALP"
BASE COUNT          446 a          948 c         1001 g          516 t
ORIGIN      
        1 gggtgacccg ttccctgact tcatcgaccc cgcgctgacc acgggcatcg aggtcgtcgg
       61 gtcgattcag acacccaacc tggagaagat cgccgcgctg aatccggacc tcatcatcgg
      121 tacccaactg gtagtcgcgc cgctgcagga cgagctcgac acgatcgcac cgacggtggc
      181 gacgcactat gcgttctacg tgtcgaactg gcgcgacgac gtgctgctcg cggccgatgc
      241 aggtggggtg ttggaccagg tgcgggccaa gctgtccgag ttcgacgcgc gggtcgagac
      301 gctgcacgca gaactgacag ccgatggcga cggtcccacg ctgtctcgtg tcgacgtgtt
      361 ctccggctcg ctggtctact accgctatgc gtgcagtgcc ttcggtgagg tgttgctgtc
      421 ggttggcatc cgtcagcccg aagcacaggc gggcgattgc accgacaacg actacacgtc
      481 ggtgttgcag tacccgtcgc tcgaacaggt cgacgtgttc gacgccgatg tgatcgtcgc
      541 ctaccagcac caggccggaa gcaccgacgt cggcgcaaac ccactcgacg tgatctccac
      601 gtcgccgctg tggggcaacc tggccgcggn nnnnnnnnna atgtccacgt cgtcggtgac
      661 cactggggct tgggcaacga gatgacggcg ttcaacctga tgctcgacga catcgaggag
      721 ttcttcggcc gatgagcgcc acgacgacca acgtggcagg catcgacgtg tctggtgatg
      781 ttcccagcga cgcgatcgat gccgccgacc cagccgcagg cgacggccgg acgcgggcgg
      841 acgggtttcg gttgttggtg ccgttcgcgc gcagcggggc ggcgcactac atcggttcgg
      901 cggtgctcgc cgcgctgggc acggtgtgcc aactcggtcc gttctggctg atctaccgcg
      961 ccgtcaccga tctggtccag ggcgacgcga cgcgcgccgg tctgatgcgg ccgtcgtggt
     1021 gcagcacgtg ctgttggccg catcgacgtg gatgtcgcac cgggccgctt tcgccacgct
     1081 ggagcaactg cggctgcgga tcggtgatcg cctcgggcgg gtgccgctcg ggttcatcac
     1141 ctcgcggcgt tcgggcgaga tccagcggac gatgaacgac gacgtcgagc ggctggagct
     1201 gttcctcgcc cacgcgatcc ccgacatcgt gtccggcgtg gtcgtggtgg tggcgacgac
     1261 cgcgtggatg ttcgcggtcg attggcgcat ggcgttggcg accgtggcga tcgtggtgat
     1321 cagcgtgccc atgatgagcg ctggcatgaa gaagggcgag gccaagctcg gcatgtacat
     1381 ggccagcctg gcgcggatga acggctcgat cgtcgagttc gtgcgcggcc tgcccgtggt
     1441 gcgcacgttc aaccgctcgg acgagacctt cgccgagacg cggtcggcga tcgaggactc
     1501 ggccgacttc cagtctcaat ggggccgcga gttcctgccg ctgttcacca ccttctacac
     1561 gctgctggcg agcaacatcg tcacgatcgt gccgatcggg ttgtggctgt ggacctccga
     1621 ccgactgtcg acgtccgacc tgctgttctt cttcatcatc gggttcggtt acccgttgcc
     1681 gctgttgaag ctgatggagt tcacctcgca gatgagctac ctcagcctca gtgccaacct
     1741 ggtcgacgag ctcgacagcg cccgcgagtt gcccgaagca ccgcagcgtg ccgagttggc
     1801 cggcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nncatccgcg agatgccgtt cgagcagctg atggagcagg
     2041 tcgcgttcgt gttccaggag acgttcctgt tcgacgacac gatcgccgcc aacctgcgcc
     2101 tggggcggcc cgacgcgacc gacgccgagg tcgaagccgt cgcgcgggca gcacgtgcgc
     2161 acgacttcat cgtcgccaaa ccagacgggt acgccacccg actgggtgaa cgcggtgccc
     2221 aactctccgg cggtgaacgg cagcggttgt cgatcgcccg ggcgatgctg aagaacgcgc
     2281 cgatcatcgt gctcgacgaa gcgacggcgt tcgccgaccc cgagaacgag gccgcgatcc
     2341 aggatgccct gaacgcgctg gccgtcgaca agaccgtggt cgtgatcgcc caccgcctgt
     2401 cgaccatcgt cggtgccgac gagatcgtcg tgctcgacgc accggccggt gagccgggac
     2461 gggtggtcga gcgcggtcac cacgccgacc tggtcgcggc gggtggtttg tactcccgga
     2521 tgtgggccgc gttcgaagac gtcgaaggca tcgagctcgg ccgtgcggtg cacgctgcga
     2581 ccgaacctgc tgcctccatc gccggggagg tcgggccatg agcatcacca tcacgaacaa
     2641 tgggacgaac cacgggacga cggaccggtt ctacgcgggc acgatccgca ccgtcatccg
     2701 cctgttcggc gaccagcgcc gggtgctcta cgtcggtatc gggttgcgga tgctcaacgc
     2761 cgtgtgcatc ggggtcggtg ctggcgtgac cgtggcggtg atccgccgcc tcggcgacag
     2821 cggtgccgat cgactcgacg gcggcgacgt gtggaccgtg accgccgtgc tcgtggcgtc
     2881 ggtgatcggc cagtacgtca ccggctactt cagcaaccgc ttcgcgtggg tgtcgacctt
     2941 ccaggccatc ggccaggcac gtctgcgcgt gctgcgtcac ctgcaacggg tgccgctcgg
     3001 ctcggtgctg tcccgccgtt ctggcgacat caccgcggtg ctgacgagcg atctggagat
     3061 ggtgtcgaac ttcgcccacc acgcgatgcc ggcgctgttc ggcgccgttg cgctgccgg
//
LOCUS       DAORNF010000383         3117 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_23598_length_3117_cov_2.154474, whole genome shotgun sequence.
ACCESSION   DAORNF010000383 DAORNF010000000
VERSION     DAORNF010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3117
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23598_length_3117_cov_2.154474"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="PLV68_14250"
     CDS             complement(<1..355)
                     /locus_tag="PLV68_14250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02462.1"
                     /translation="MSLLAATIVVLSAIVAFGTTQAAVGADTTTTTPPTTTTPAPVIP
                     LAIFPLQGKCSFTDTFAAARSGGRTHEGVDLIAKMGQFIYAAADGTLTKKYIDAPGSL
                     SGNGWRLTKADGTYFF"
     gene            519..1562
                     /locus_tag="PLV68_14255"
     CDS             519..1562
                     /locus_tag="PLV68_14255"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRE02463.1"
                     /translation="MLVSGMGSELGSLVATKLEAEPWVGALEGIDVDPPRRRLRRAMF
                     HRIDPHDRERVFDVVRAFDPHIVVHIAVWEPNARANTSHAQTLTNAAAVSILGAAAEC
                     PSLEHTIVRSAAEIYGRGRGALTRPDEDAPPSPTSTFGRMAAMIESTAGDAARRTGTG
                     LGAVRLAPVLGRHVPSPLGRLLRLPAVPFSALADPPFGVVHDHDAADALVAAARTRLS
                     EPVNVTAPGAITALQALLRGRRVPIPLLGPEWAIARRISNLAGAPIPDHVAETMHRGR
                     LIDGSRAQELLGVAPTTTTAEVIDTLYAWPSVIHIPAQPSTPAEAAETQSGAGSRARV
                     TPKTRSAKKGVAS"
     gene            1592..2266
                     /locus_tag="PLV68_14260"
     CDS             1592..2266
                     /locus_tag="PLV68_14260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02464.1"
                     /translation="MLRRAGRALSAPVAVGRSAPLVDEWGRAQSLIDVVMPLLFARWD
                     VSVGGSEHLPRHGGALIVANARRFAHTSIYAALSIGDTVGRPVRFAGRSDMAPLGALA
                     RRIGGLLEHPDEVSGALRAGELVLITTAATGSSAHAGTVDPAMMRAAARAGVAVHPAA
                     TESSPTSREARVEIAAPVVTERPRRGPLAEADMADAVQRRLQELLDEMGDARPGVPVL
                     DWLRGS"
     gene            2268..>3117
                     /locus_tag="PLV68_14265"
     CDS             2268..>3117
                     /locus_tag="PLV68_14265"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRE02465.1"
                     /translation="MPYARSDDGVRLHYEVTGRRSGPPVLLIQGLGADKHGWDLQRLA
                     LASRYRTIALDNRGAGRSDKPFGDYSLEQMADDAVAVLDELDVDSAHVVGASMGGAIS
                     QVLALKHPQRVRSLTLACTACRHHPWRRELLGEWATTATEQGMGTMAREAARWVIGPR
                     SFRRVLPLLGWFGPLAIGRPAHAFVAQVRAILATDDAMADELATLDVPTLVLVGNQDI
                     LTPRGDSEELAERIPGARLAVISGAAHGLMIEHATTFNRLLIEFLDETVAATANHPHL
                     TLVASEP"
BASE COUNT          451 a         1138 c         1076 g          452 t
ORIGIN      
        1 agaagaagta ggtaccgtcg gccttggtca accgccagcc gttgcccgac agcgagcccg
       61 gcgcgtcgat gtacttcttg gtcagcgtgc cgtcggccgc ggcgtagatg aactggccca
      121 tcttggcgat gaggtcgacg ccttcgtgtg tgcgcccccc cgagcgcgcc gcggcgaagg
      181 tgtcggtgaa cgagcacttg ccctgcaacg gaaatatggc cagcgggatc accggtgccg
      241 gggtggtcgt cgtgggcggc gtcgtggtcg tcgtgtcagc tccgacggca gcctgtgtcg
      301 ttccgaatgc aacgatcgcg ctcagcacga cgatcgtggc cgcgagcagg ctcacgacag
      361 caatcgccgg agcggcaaga cgaacacgac cagggagagg tatcggcgcg tcgtaccgcc
      421 cgcttgagcc gcccactcga agcgctgcga gctgtgccgc ccggcccgag acccgacgct
      481 gacgtcggat aggttcgtga accgtgcgcg ggcaacgagt gttggtatcc ggaatgggca
      541 gtgagctggg gtcgttggtg gcgaccaagc tcgaggccga gccgtgggtc ggcgcccttg
      601 agggcatcga cgtcgatcca ccgcgacggc gcttgcggcg ggcgatgttc caccgcatcg
      661 acccccacga tcgcgagcga gtcttcgacg tcgtgcgcgc gttcgatccc cacatcgtgg
      721 tgcacatcgc cgtgtgggag cccaacgcgc gagcgaacac gtctcatgcc caaacgttga
      781 ccaacgcggc cgcggtgtcc atcctgggcg cggccgccga gtgcccgtcg ctcgagcaca
      841 ccatcgtgcg cagcgcggcc gagatctacg ggcgcggccg cggcgcactg acccgtcccg
      901 acgaggatgc tcccccgtcg ccgacctcga cgtttgggcg gatggccgcg atgatcgagt
      961 ccaccgcggg cgacgcggcc cggcggaccg gcaccggtct cggtgcggtc cgactggcac
     1021 cggtgctcgg ccgtcacgta ccgagcccgc tcggccgcct gctgcgcctt ccagcggtgc
     1081 cgttcagcgc gttggccgat cctcccttcg gtgtcgtcca cgaccacgac gcggctgatg
     1141 ccctcgtcgc cgcggcccgc acccgcctga gcgaaccggt caacgtgacc gcgcccggag
     1201 cgatcacggc gctgcaggcg ctgttgcgcg gccggcgggt tcccatcccg ctgctcgggc
     1261 cggaatgggc catcgcccgg cgcatcagca acctggccgg cgcgccgatc cccgaccacg
     1321 tcgccgagac gatgcaccgt ggccgcctga tcgacggtag ccgagcccag gaactcctcg
     1381 gcgtcgcgcc caccaccacg accgccgagg tgatcgacac gctgtacgcg tggccgtccg
     1441 tgatccacat cccagcccaa ccgtccacgc ccgctgaggc ggccgagacc caatcgggag
     1501 ccgggtcacg agccagggtg acgccaaaga ctcggtccgc caagaagggg gtggcgtcgt
     1561 gaacgcgtca gcgccatcaa cacgaccatc ggtgctccgc cgcgccggac gcgcgctgag
     1621 cgctcccgtc gcggtcgggc gctcggcccc gctggtcgac gaatgggggc gagcccagtc
     1681 actgatcgac gtggtgatgc ctctgctctt cgcgcggtgg gacgtcagcg tcggcggcag
     1741 cgagcacctg ccacgtcacg gcggtgcgct gatcgtcgcc aacgctcgcc ggttcgcgca
     1801 cacgtcgatc tacgccgcgc tgtcgatcgg cgacacggtg gggcgccccg tgcgcttcgc
     1861 cggtcgatcc gacatggcac cgctgggcgc attggcacgc cgcatcggtg gcctactcga
     1921 acatcccgac gaggtctccg gggcgttgcg agccggtgag ctggtgctga tcaccaccgc
     1981 cgccacgggg agttcggcgc atgccggaac cgtcgacccg gcgatgatgc gtgccgccgc
     2041 ccgcgccggt gtggccgtcc atcctgccgc gaccgagtcg tcgccgacat cgcgggaggc
     2101 ccgggtcgag atcgccgctc cggtcgtgac cgaacgaccc cggcgtggcc cgttggccga
     2161 agccgacatg gccgacgcgg tgcaacgacg gctgcaagag ctgctcgacg agatgggtga
     2221 cgctcgcccc ggcgtgccgg tgctcgactg gttgaggggg tcctgacgtg ccgtacgcac
     2281 gctccgacga cggtgttcgc ctgcactacg aggtcaccgg gcgccgcagc ggtccgcccg
     2341 tgttgctgat ccagggcctc ggtgccgaca agcacggctg ggacctgcaa cgcctggccc
     2401 tcgcatcgcg ctatcgcacg atcgcgctcg acaaccgggg cgcgggccgc agcgacaagc
     2461 ccttcggcga ctactcgctc gagcagatgg ccgacgacgc cgtcgcggtg ctcgacgagc
     2521 tcgacgtcga ttcggcccac gtcgtcggcg cgtcgatggg tggtgcgatc agccaggtgc
     2581 tcgcactgaa acatccccag cgggtccgct cgctgacgct ggcctgcacc gcctgccgcc
     2641 accacccgtg gcggcgcgaa ctgctcggcg agtgggccac caccgcgacc gaacagggca
     2701 tgggcacgat ggcccgtgag gccgccagat gggtgatcgg gccacggtcg ttccggcgag
     2761 tgctcccgct gctgggctgg ttcggcccgc tcgcgatcgg ccgccccgcc catgccttcg
     2821 tggctcaggt gcgggcgatc ctggccaccg acgacgcgat ggccgacgaa ctcgccacgc
     2881 tcgacgtgcc gacgctggtg ctggtcggca accaggacat cctcaccccg cgcggcgact
     2941 cggaggagct ggccgagcgc atccccggcg cccgcctggc cgtgatctcc ggcgcggccc
     3001 acgggctgat gatcgagcac gccaccacct tcaaccggtt gctgatcgag ttcctcgacg
     3061 agaccgtcgc cgccaccgcc aaccaccccc acctcaccct ggtcgcatcc gagccgg
//
LOCUS       DAORNF010000384         3110 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_23658_length_3110_cov_1.830769, whole genome shotgun sequence.
ACCESSION   DAORNF010000384 DAORNF010000000
VERSION     DAORNF010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3110
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23658_length_3110_cov_1.830769"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    242..409
                     /estimated_length=168
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<410..620)
                     /locus_tag="PLV68_14270"
     CDS             complement(<410..620)
                     /locus_tag="PLV68_14270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02466.1"
                     /translation="MTHHDTHHARSYDPNGFSKPELEFFDPDGAPGFPWTPLAFDRTG
                     QLSEIILSGSHESGWVSRLLKFVPGA"
     gene            complement(744..>1369)
                     /locus_tag="PLV68_14275"
     CDS             complement(744..>1369)
                     /locus_tag="PLV68_14275"
                     /inference="COORDINATES: protein motif:HMM:NF015214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase C-terminal
                     domain-containing protein"
                     /protein_id="HRE02467.1"
                     /translation="SGQFQRTAAHSLGNSQAQTRFATKLAFFAGLGRALYENSGVIND
                     PKNKARLGQLAGQCHLPEAFLQAAERNWFLDGYGVANPDHATLYAAMTMQPKLMNEII
                     FLLREMSGGSVIQLPSSVAAYDHATCAADIDRYIRWPNASSDERVALLNLVWDAIGSE
                     FAGRHLQYEMFYAGDPSVVQMRSFGSYHWDRSRTLVDGVLNRSAAPT"
     assembly_gap    1370..1421
                     /estimated_length=52
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1422..>1849)
                     /locus_tag="PLV68_14280"
     CDS             complement(<1422..>1849)
                     /locus_tag="PLV68_14280"
                     /inference="COORDINATES: protein motif:HMM:NF023222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HRE02468.1"
                     /translation="FAEGGQQFADNVQRFYERARDESLYLSYTIIHPTVDRTKPPHQQ
                     YIPNLYVSVLREQDDGIVLQGAQMLGTGSVMSDYIFVSCILPLPEGAEDYAFSVVVPA
                     SAPGLRIYSRRSYADATRSNVDHPLAGRFDETDSLVVFDE"
     assembly_gap    1850..1930
                     /estimated_length=81
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1931..2209)
                     /locus_tag="PLV68_14285"
     CDS             complement(<1931..2209)
                     /locus_tag="PLV68_14285"
                     /inference="COORDINATES: protein motif:HMM:NF023222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HRE02469.1"
                     /translation="MRTGADFLASVRDGRHVILDGEVVDDVSTHPAFSGVVHTMADMY
                     DAAADPANRDLYTYPSPSDGSPVHTWWMIPRDEADLAQRRRAIAAWSAL"
     gene            complement(2209..2988)
                     /locus_tag="PLV68_14290"
     CDS             complement(2209..2988)
                     /locus_tag="PLV68_14290"
                     /inference="COORDINATES: protein motif:HMM:NF022463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2848 family protein"
                     /protein_id="HRE02470.1"
                     /translation="MASQFVVPQFVACQLAESRSGSPMKFSVVGTERDVALDAVIIAG
                     YTGRDRDAVMHHIDELAALGVPPPPTVPMYWMLPGWIATQQPLTTATGAGSSGEAEMC
                     LIVDGAPQDGGPIDPANVFVTIASDHTDREAEAVDIGLSKAICQKPMGVDAWPLADVA
                     GRWDELVLRSWIDDGNGEVAYQHGPCSTLVPPTDLLAGVPFDPRPQRFALLCGTVPVI
                     GGIRPSGWFRGELHDPKLRRSIEFQYEVRALTPARPKAGDE"
BASE COUNT          469 a          894 c         1014 g          432 t
ORIGIN      
        1 cccggccagt tccagatggg gcgacacctc gcgtgtcacg gcggtgatcc gcccggctgc
       61 ggccatgcgt tccagtacgg cgcggaacga caggtcgagg ggaccgagcg cgccagacgg
      121 accagaacca gaaccagaac cagcgatgcc agacacaggg ttgaccgtca cggcgcgatc
      181 ctccctcagg actcgggcgc ggccatgccg gggcgtggct cggggaagcg ggtctcgaag
      241 gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc ggcgccgggc
      421 acgaacttga gcaggcggct cacccatccg ctctcgtgcg agccggacag gatgatctcg
      481 ctcaactggc ccgtgcggtc gaaggccagc ggcgtccacg gaaacccggg cgcgccgtcg
      541 gggtcgaaga actcgagttc gggcttggaa aacccgttgg gatcatacga acgagcgtga
      601 tgggtgtcgt ggtgggtcat gtcggttctc ttcgctgggt gggtcggcgg tgcggggatg
      661 gttgtgctcg tcgcggggac gatccgtcgg cgagcaagcg tggtggaccg ttcgatggga
      721 cgggccaagc ggatggtgac agatcaggtc ggcgcggccg aacggttgag cacgccatcg
      781 acgagggtac gcgagcgatc ccagtggtaa ctgccgaagc tgcgcatctg tacgaccgac
      841 gggtcgcccg catagaacat ctcgtactgc agatggcgtc ccgcgaactc ggagccgatc
      901 gcgtcccaca ccaggttgag gagggcgacc cgctcgtcgg acgacgcgtt cggccagcgg
      961 atgtagcggt cgatgtcggc cgcgcatgtc gcatggtcgt aggcagcaac cgacgacggc
     1021 agctggatca ccgaaccgcc gctcatctcg cgcagcagga agatgatctc gttcatcagc
     1081 ttcggctgca tcgtcatcgc cgcgtacaac gtggcgtgat ccgggttggc gacgccatag
     1141 ccgtcgagga accagttccg ctcggcggcc tgcaagaacg cttcgggcag atgacactgc
     1201 ccggcgagct ggccgaggcg agccttgttc ttgggatcgt tgatgacgcc gctgttctcg
     1261 tacagcgccc ggccgagccc ggcgaagaag gccaacttgg tggcgaagcg cgtctgggcc
     1321 tgactgttgc ccagcgagtg ggccgcggtg cgctggaact ggccgctcgn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncctcgtcga acaccaccag
     1441 cgagtcggtc tcgtcgaacc gcccggcgag gggatggtcg acgttcgagc gcgtcgcatc
     1501 ggcgtacgac cggcgggagt agatccgcag accgggtgcg ctggcgggca cgacgacgct
     1561 gaaggcgtag tcctcggcgc cttcgggcag cggcaggatg cagctgacga agatgtagtc
     1621 gctcatcacc gacccggtgc cgagcatctg cgcaccctgc agcacgatgc cgtcgtcctg
     1681 ctcgcgcaac acgctgacgt acaggttggg gatgtactgc tggtgcggtg gcttggtgcg
     1741 gtcgacggtg ggatggatga tcgtgtagct gaggtacaag ctctcgtcac gggcccgctc
     1801 gtagaagcgc tggacgttgt cggcgaactg ctggccaccc tcggcgaacn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn cagcgccgac caggctgcga tcgcacgacg gcgctgggcg agatcggcct
     1981 cgtcacgggg gatcatccac cacgtgtgca cggggctgcc atcgctgggc gaggggtagg
     2041 tgtagaggtc gcggttggcc gggtcggccg ccgcgtcgta catgtcggcc atcgtgtgca
     2101 ccacgcccga gaaggcgggg tgggtcgaga catcgtcgac gacctcgccg tcgaggatca
     2161 cgtggcggcc gtcgcggacg gacgcgagga agtcagcacc tgtgcgcatc actcatctcc
     2221 tgccttgggg cgcgccggcg tcagcgcgcg aacttcgtac tggaactcga tcgaccggcg
     2281 cagcttcggg tcgtgcagct caccgcggaa ccagcccgac ggacggatgc cgccgatgac
     2341 gggcacggtg ccgcacaaca gcgcgaagcg ctgtggccgc ggatcgaagg gcacgccggc
     2401 gagcagatcg gtgggtggca ccagcgtcga gcagggcccg tgctggtagg cgacctcgcc
     2461 gttgccgtcg tcgatccaac tgcgcaagac gagctcgtcc cagcgacccg cgacgtcggc
     2521 caacggccac gcgtcgacgc ccatcggctt ctggcagatc gccttgctga ggccgatgtc
     2581 gaccgcctcg gcctcgcgat cggtgtggtc ggatgcgatc gtgacgaaca cgttcgccgg
     2641 gtcgatcggg ccgccatcct gcggcgcgcc gtcgacgatc aggcacatct ctgcttcgcc
     2701 cgacgaccca gcgccggtcg cggtcgtcag cggctgctgc gtcgcgatcc agcccggcag
     2761 catccagtac atcggcaccg tgggcggcgg cggcacaccc agggcggcca gctcgtcgat
     2821 gtggtgcatg acggcatcgc ggtcgcgacc ggtgtagccc gcgatgatca cggcgtcgag
     2881 cgcgacatca cgctcggtac ccacgacgga gaacttcatc ggcgatccac tccttgactc
     2941 ggccaactga catgcgacga actggggcac gacgaactgt gacgccaagg actggccggt
     3001 tcgcggcggg cgctggcatc atcgatgcca tgcacggaaa cgaggccagc catacgaacg
     3061 cagccccgcg gcgcgtcgtg atcggggtga cgggcgcgag cggatcggtg
//
LOCUS       DAORNF010000385         3107 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_23692_length_3107_cov_1.635976, whole genome shotgun sequence.
ACCESSION   DAORNF010000385 DAORNF010000000
VERSION     DAORNF010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3107
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23692_length_3107_cov_1.635976"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            40..>273
                     /locus_tag="PLV68_14295"
     CDS             40..>273
                     /locus_tag="PLV68_14295"
                     /inference="COORDINATES: protein motif:HMM:NF015232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HRE02471.1"
                     /translation="MYLSNEANSVNWLMASFVERTIGARQAIAVSSDGLLIAMSATVD
                     RASADKLAAIVTGMRALAHGAAHELASGPVVQVL"
     assembly_gap    274..390
                     /estimated_length=117
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            624..>977
                     /locus_tag="PLV68_14300"
     CDS             624..>977
                     /locus_tag="PLV68_14300"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF017170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HRE02472.1"
                     /translation="MQCAGAVRIDHAMALQRLFWIPGEAGAAGGSYVRYPFESMLQTT
                     ARGVTMLPALNFERLAITEMCAAEILSVAELSARLGLPIGVVRVVAGDLLADGLLEAF
                     MSNLSVADDVSLIMRL"
     assembly_gap    978..1108
                     /estimated_length=131
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1109..>1367
                     /locus_tag="PLV68_14305"
     CDS             <1109..>1367
                     /locus_tag="PLV68_14305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HRE02473.1"
                     /translation="DIQPLTTEAAMTTVADGVDDRGQVSQKTTTTVAMDFGRVKVDDS
                     IVLYIFGTPGQDRFGFMWNDLVHGALGAVVLVDSRRIEDSFP"
     assembly_gap    1368..1490
                     /estimated_length=123
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1631..2380)
                     /locus_tag="PLV68_14310"
     CDS             complement(1631..2380)
                     /locus_tag="PLV68_14310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02474.1"
                     /translation="MVALAVMATACRVDVSVDVAMEEDGSGIITVAAMADAELVSRAP
                     SIATDLRFDDMQAAGWTVEGPIPTQADGIQVRLTHEFSTPAQANALLAQISGTDGPFA
                     DLIVSRRVVERTTTYSITGHLGPVVGLEAFSDADLTAALGAVPYADQLASSKVEPGEV
                     IGIIVTASLPGKIDDTTTASELRWAVPFDGSTVQVATTSKVVEPENTWIRPLASGLKI
                     ALFAWIMLSLVGFAYVFLLRRNQRIENQPWY"
     gene            complement(2502..>3107)
                     /locus_tag="PLV68_14315"
     CDS             complement(2502..>3107)
                     /locus_tag="PLV68_14315"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRE02475.1"
                     /translation="PQRYGAVIGLSGALIENGSSRALALGYLFGATLVIGAAVMMGLQ
                     PRLTRWLAARRANVVTASAAPQTADGATAEPATTDTAAPDRVGFGAWLATFLTGVYGG
                     YFGAAQGIILITALAILLPDDLRRTNALKNALALVVNGVASLFFIVVADVAWEPAALI
                     AVGAIVGGTLGARVARRINQRVLRSIIVIGGIAIGIRLIVT"
BASE COUNT          431 a          876 c          978 g          451 t
ORIGIN      
        1 ccagcagtga cagccacgca ggaacagaag ggcagtccga tgtacctctc caatgaagcg
       61 aacagtgtca actggctgat ggccagcttc gtcgagcgca cgattggcgc tcgccaggcg
      121 atcgcggtct cctccgacgg tctgctgatc gcgatgtcgg ccaccgtcga tcgggcctct
      181 gccgacaagc tggcggcgat cgtcaccggc atgcgtgcgc tcgcccacgg cgcagcgcac
      241 gagctcgcca gcggccccgt cgtgcaggtg ctgnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gggtgggtgc ccagctcacc ccggcactgg
      421 tgaccgagct gaagaactcg ctcgtcagcg tgtgaccatg tctgcgttcg cgagtgcggg
      481 gcgaccggag ccgggaagtg ctcaacggcc gggggacgtg gccgacgaga cgccggccga
      541 cgacaccggc accttggtgc gtccgtttct tgcccggccg atgttggcga cggcgatgtc
      601 ggtgacgggt aacgagcgtg acgatgcagt gtgcaggcgc cgtgcgcatc gaccatgcca
      661 tggcgctgca gcggctgttc tggatcccgg gcgaggcggg cgcggcaggc ggcagctatg
      721 tccgctaccc gttcgagtcg atgctgcaga ccacggcgcg cggcgtcacg atgctgcccg
      781 cgttgaactt cgagcggctg gcgatcaccg agatgtgtgc cgccgagatc ctgtcggtcg
      841 cggagttgtc cgcccggctg ggactgccga tcggcgtggt gcgagtggtg gccggcgact
      901 tgttggccga tggcttgctc gaagcgttca tgtccaacct gagcgtcgct gacgacgtct
      961 cgctgatcat gaggttgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnncc gacatccagc cgctgaccac cgaagccgcg
     1141 atgaccacag tggccgacgg cgtcgacgat cgcggccagg tgtcgcagaa gaccaccaca
     1201 acggtggcga tggacttcgg ccgggtcaag gtcgacgact cgatcgtgct ctacatcttc
     1261 ggtacacctg gccaggatcg atttggtttc atgtggaacg acttggtgca tggcgcgctc
     1321 ggtgccgtgg tgctggtcga ttcgcgtcgg atcgaggact cgtttccnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ccgcacgtcc
     1501 ccgttgtagc gaccgacgcc cgacagcgca gcgccgtcaa ggacaccgtg ctgcgcctgc
     1561 tgaccgtgtc gctggaacac gcccgcgccc gcgccggtct gtagcgccgg cccggccggg
     1621 cagaacctgg tcagtaccag ggctggtttt cgatgcgctg gttgcggcgc aggaggaaca
     1681 cgtaggcaaa gcccaccagc gacagcatga tccaggcgaa gagcgcgatc ttcaagccgc
     1741 tggccaaggg tctgatccag gtgttctcgg gctcgaccac cttggaggtc gtggccacct
     1801 gcacggtcga accgtcgaac ggcacggccc agcgcagttc gctcgccgtc gtggtgtcgt
     1861 cgatcttgcc gggcagtgag gcggtgacga tgatgccgat cacctcgcct ggttcgacct
     1921 tggacgaggc gagctgatcg gcgtacggca cagcaccgag ggcggcggtg agatcggcgt
     1981 cgctgaacgc ctccagcccg acgaccgggc cgaggtggcc ggtgatgctg taggtcgtgg
     2041 tgcgctcgac gacgcgccgg ctgacgatca ggtcggcgaa cggtccgtcg gtaccgctga
     2101 tctgggccag cagggcgttg gcttgggccg gtgtggagaa ctcgtgggtg aggcgaactt
     2161 ggatgccgtc ggcctgggtg gggatcggtc cctcgacggt ccagcccgcg gcctgcatgt
     2221 cgtcgaaacg gaggtcggtg gcgatgcttg gcgcccggct gaccagctcg gcgtcggcca
     2281 tggcggcgac ggtgatgatg ccgctgccgt cctcttccat cgcgacgtcg acgctgacgt
     2341 cgacgcggca cgccgtggcc atcacggcca gcgccaccac cagcatcacc ctctgcaccc
     2401 gtcgcgacat gaggtctcag tctcgcatct ccgcggcctc gcgcggtgaa cgcggcgaag
     2461 ttctgccgcg atcgtgatgg tgcgatcgcg accgccgcgg ttcaggtgac gatcagacgg
     2521 atgccgatcg cgatgccgcc gatcacgatg atcgagcgca ggacccgctg gttgatgcgc
     2581 cgcgcgacgc gggcgccgag cgtgccaccg acaatggcac cgacggcgat cagcgcggct
     2641 ggttcccagg ccacgtcggc gacgacgatg aagaacaggg acgcgacgcc gttgaccacc
     2701 agcgcgagcg cgttcttgag ggcgttggtt cggcgcaggt cgtcgggcaa caagatcgcc
     2761 agggcggtga tgaggatgat gccctgcgcg gcaccgaagt acccgccgta cacgccggtc
     2821 aggaacgttg ccagccacgc gccgaaacca actctgtcgg gagcggccgt gtcggtcgtc
     2881 gccggttcgg cggttgcccc atcggccgtc tggggagctg cgctggcggt gacgacgttg
     2941 gcccggcggg cagcgagcca ccgggtgagg cgtggttgga gccccatcat cacggcggca
     3001 ccgatcacca gcgtggctcc gaacagataa ccgagagcaa gtgctcgcga cgagccgttc
     3061 tcgatcaggg cgccgcttag tccgatcacg gcgccgtaac gctgggg
//
LOCUS       DAORNF010000386         3106 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_23699_length_3106_cov_1.809571, whole genome shotgun sequence.
ACCESSION   DAORNF010000386 DAORNF010000000
VERSION     DAORNF010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3106
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23699_length_3106_cov_1.809571"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            58..>1641
                     /locus_tag="PLV68_14320"
     CDS             58..>1641
                     /locus_tag="PLV68_14320"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRE02476.1"
                     /translation="MVRLHPKPFVIAVLGAAVFALCTVASSIAVQWVVDHVIEPRFRK
                     GHVAAGTVLTGLAIVVGIGLIRAAGIVVRRAWAGKAHWRIGETMSGQVVDRLVAQPVA
                     WHRQHQSGDLAARGAVDVDAATDVLSPLPFASSVVLMVFVAAVWLVVIDLPLGVLSVA
                     VFPLLIAINVMYQHRVDAYYTQAQDHLGDLSAAVHESFEGVQVVKAFGAEARETDRLA
                     LIAARLRDARISAVMLKGIFDAVLDAIPALANAAIVLVGACRIRSGDLTIGELASVIY
                     MFTLLVFPLRLIGFALSALPHSLAGYNRVQEIVREPLWPDPARTVAVAPSEVALSMRG
                     VSYEFEPGDPVLREVDLTVVPGSTVALVGATGSGKTTLLRLAAGLIPASAGHVAAVAG
                     TRAIVLQEAFLLGDTLRGNITLGEAFSDDEVWAALDTAEGRTFVDELPRGLDTEVGER
                     GVSLSGGQRQRVALARALVRQPRLLLMDDTTSALDPATEARVVAKLTTALRGASVLMV
                     ASRPSTIALADQVVFIADGA"
     assembly_gap    1642..1692
                     /estimated_length=51
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1765..2922
                     /locus_tag="PLV68_14325"
     CDS             1765..2922
                     /locus_tag="PLV68_14325"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRE02477.1"
                     /translation="MDVVDDELGGRLGTARILTRGVHEAPVLRQGFGVTFLLAGLGAA
                     GRVVIPITIQQAIDHGFPGGQVRIGFIVTLVVIAAAAIIAGGIGQAMAVRRLGRRSEE
                     ALYDLRVRVIAHIHRLSLADHSEERRGALVARVTADVETLANFFQWGGLAFLINGAMM
                     VLVAAVMLAYNWLLAVIAFAVALPLAWVLHAVQSRLASAYDRARERNGELVGSMSELV
                     SGAETLRAYSAGPLMARRTKRVAKERADSFIRAGVIGAFLFPSGEVFGAFTVAAVVGV
                     GVARGSAGGLTAGALVGFVFLTQRFLEPIAEFTEVLDQAQTAVSGLRRLLGVLDIPAG
                     PPSSANPRALPEGPLTIEIDDVTFRYRSRGEAEDDDPVLIGVSAVIPAGGC"
BASE COUNT          392 a         1024 c         1111 g          528 t
ORIGIN      
        1 cgtctgagcg ccggtcgggc ggcacggtgg cgcgccgctg cacagctgat ccgcaatatg
       61 gttcgcctcc accccaagcc gttcgtcatc gccgtgctcg gggcagcggt gttcgcccta
      121 tgcaccgtcg cctcgtcgat cgcggtgcaa tgggtggtcg accacgtgat cgagccccgc
      181 ttccggaagg gccacgtcgc ggccggcacg gtgttgaccg gtctcgccat cgtcgtcggc
      241 atcggcctga tccgcgccgc cggcatcgtc gtccgccggg cctgggccgg caaggcccat
      301 tggcgcatcg gcgagacgat gagcggtcag gtggtcgacc ggttggtcgc ccagccggtc
      361 gcctggcacc ggcagcacca gtcgggcgat ctcgccgcgc gtggtgccgt cgacgtcgac
      421 gcggcgaccg acgtgctctc gccgctgccg tttgcgagca gcgtcgtgct gatggtgttc
      481 gtggctgccg tgtggctggt ggtcatcgat ctgccgctcg gggtgttgtc ggtggcggtg
      541 ttcccgctgc tgatcgcgat caacgtgatg taccagcacc gcgtcgacgc ctactacacg
      601 caggcccagg atcacctcgg cgacctgtcg gcggcggtgc acgagagctt cgaaggcgtg
      661 caagtggtca aggccttcgg cgccgaggcg cgcgagaccg accggctggc gctgatcgct
      721 gctcgtctgc gcgacgcgcg catctcggcg gtgatgctga agggcatctt cgacgctgtc
      781 ctcgacgcga tccccgcgtt ggccaacgcc gcgatcgtgt tggtcggcgc gtgccgcatc
      841 cgcagcggcg acctcaccat cggcgagctg gccagcgtga tctacatgtt caccttgctg
      901 gtgttcccgc tccgcctgat cggcttcgcc ctgtcggcac tgccgcactc gttggcgggg
      961 tacaaccggg tgcaagagat cgtccgcgaa ccgctgtggc ccgacccggc gcgcacggtt
     1021 gctgtcgccc catccgaggt ggcgctgtcg atgcgcggtg tcagttacga gttcgaaccg
     1081 ggtgatccgg tactgcgcga ggtcgatctc acggtcgtgc ctggctcgac cgtggcgctg
     1141 gtgggcgcga ccggtagcgg caagacgacg ttgttacgtc tggcggccgg gctgatcccg
     1201 gcctcggccg ggcacgtcgc cgcggtcgcc gggacccggg cgatcgtgct gcaagaggcg
     1261 ttcctgctgg gggacacgct gcgcggcaac atcacgctgg gcgaggcgtt ctccgacgac
     1321 gaggtctggg ctgcgctcga caccgccgaa gggcggacct tcgtcgacga actcccccgc
     1381 gggctcgaca ccgaggtcgg cgagcggggt gtctcgctca gtggtgggca gcgccagcgg
     1441 gtggcgctcg cgcgggcctt ggtgcgccag ccccggctgc tgctgatgga cgacaccacg
     1501 tccgcgctcg accccgcgac cgaggcgcgc gtcgtcgcca agctcaccac agcgctgcgc
     1561 ggcgcgagcg tgctcatggt ggccagccgg ccgagcacca tcgcactggc cgatcaggtg
     1621 gtgttcatcg ccgacggagc cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nncatggaag cgttcgaggt cgaccgtgag accgcgggcg aggccggagc
     1741 ggtcggtgcc gggggacagg gctgatggac gtcgtcgacg acgaactcgg tggacggctc
     1801 ggcaccgcgc ggatcctgac ccgcggcgtg cacgaagcgc ccgtcctgcg ccagggcttt
     1861 ggggtcacgt tcctgctcgc cgggctcggc gcagcgggac gtgtcgtgat cccgatcacc
     1921 atccaacagg cgatcgacca cgggtttcct ggtggtcagg tgcggatcgg cttcatcgtc
     1981 accctcgtgg tcatcgccgc ggcggccatc atcgccggcg gcatcggcca ggcgatggcc
     2041 gtgcgccgcc tcggccggcg cagtgaagag gcgctgtacg acctgcgtgt ccgggtgatc
     2101 gcccacatcc accggctgag cctggccgac cacagcgaag agcgccgcgg tgcgctcgtc
     2161 gcccgtgtca ccgccgacgt cgagacgctg gccaacttct tccaatgggg cgggctcgcg
     2221 ttcttgatca acggggcgat gatggtgctc gtcgcagccg tcatgctggc ctacaactgg
     2281 ctgctcgccg tgatcgcgtt cgccgtcgcg ttgccgctgg cgtgggtgct gcacgcggtg
     2341 cagagccggc tcgcgtcggc gtacgaccgg gcgcgcgaac gcaacggcga actggtcggg
     2401 tcgatgagcg agttggtgtc cggagccgag acgttgcgcg cctacagcgc ggggccgttg
     2461 atggcgcggc gtaccaagcg ggtggccaaa gagcgcgcag attcgttcat ccgcgccggt
     2521 gtgatcgggg ccttcctgtt cccgtcgggc gaggtgttcg gggccttcac cgtggccgcg
     2581 gtcgtcggtg tcggcgtcgc tcgcggcagc gcgggcgggc tcaccgcggg agcgttggtc
     2641 ggcttcgtct tcctgaccca gcgcttcttg gagccgatcg ccgaattcac cgaggtgctc
     2701 gaccaggccc agacggcggt gtccggtctg cggcggttac tcggggtgct cgacatcccc
     2761 gccggtccgc cgtcgtcggc caacccgcga gcgttgcccg aggggccgtt gacgatcgag
     2821 atcgacgacg tcacgttccg ctatcgctca cgaggcgagg cggaggacga cgatccagtg
     2881 ctgatcggtg tgtcggccgt gatcccggcc ggcgggtgct gaagggccgg atcggccgcg
     2941 gcgccgtcga caagatcaat gccgtgctgt ggttctccga cctgcatggc ttcaccaagc
     3001 tgtcggagac gatggcctcg gatcagctca ttccgttcct caacgaccac gccgatgcgg
     3061 tcatctcggc gatccaggcg tccggtggcg acgtgctgaa gctgat
//
LOCUS       DAORNF010000387         3086 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_23894_length_3086_cov_2.048829, whole genome shotgun sequence.
ACCESSION   DAORNF010000387 DAORNF010000000
VERSION     DAORNF010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3086
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23894_length_3086_cov_2.048829"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..1911)
                     /locus_tag="PLV68_14330"
                     /pseudo
     CDS             complement(1..1911)
                     /locus_tag="PLV68_14330"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
     gene            2078..>2816
                     /locus_tag="PLV68_14335"
     CDS             2078..>2816
                     /locus_tag="PLV68_14335"
                     /inference="COORDINATES: protein motif:HMM:NF015297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HRE02478.1"
                     /translation="MRSLLFIPGNRPDWIGKAAASGADAVVFDLEGSLPAADKESGRR
                     GVRAVLDAADPNGPAIFVRVNDAHGGLMPADLDVLVGPNLAGVLLPQVRSVEEVRDLA
                     DRLDALEAERGLEHGTVAVDPLMESPQALYRAYEIATCADRIAYMGSGISRRGDIVRT
                     MGYRWTPEGHETLYFRSKVLLEVRAAGVVNPLSGMWGDVADLGGLRRLADQTRDIGYE
                     GMMVIHPTHVPVVNEVFSADPAELEHFR"
     assembly_gap    2817..2847
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          449 a          994 c         1096 g          516 t
ORIGIN      
        1 cgcatcggct tgcaggaaca cccagccgtc gccggtctcg tacaggcggt gcagggcatg
       61 gaagccgtgc tggccgccgt cggggaacgg ccccggcgtc ttgccgtcgt agcgcacgct
      121 ccagcgcgac acggtgtagg ccatcgacga cagcatcgtc gtctcgacgt actgcccgag
      181 tcctgtgcgc tcgcgggcgg cgagaccgag catggtcgcg gtcgcgcagc cgagcgcacc
      241 tgccgggtcg gcgtaggtgc ggttgatcgg cgagttgccg gcaccggcct agatgatgcc
      301 cgaaccggcg atcgcgttgg gtgaggagtg gaacccgggg cgggcgaacc acggtccctt
      361 ggagccgaag caaccgccgt agacgtagac gagccgaggg ttgatcgcct tgagctgctc
      421 gtagccgatg ccgagccgtt cggggacgcc ggggcggaag ttgtgcagga agacgtcggc
      481 ccgtgcggcg aggtcgagca cgatctgcag gccctcgggt gtcttcatgt cggcgacgac
      541 gctctccttg ccgaccgtct ccttgtcgta gtgcgtcgcc cagttgcgcc gcgccgggtc
      601 gcctgccggt ggctcgacct tgatcacgcg cgcgcccaac tcggccagca acgtcatcgc
      661 gaacggggct gcgtagaagt agccgacctc gagcacggtg atgccgctca gcggagcatc
      721 cggcaagggg gtgccggggg agaggtttgc cgacgtgtcg gcgctgctct cggtggggcg
      781 gggacgcggt gcggcggcga gctcggcccg gatcgcctcg ccgtgctcgt cgagggcggg
      841 ttcgcccgcg gcgatcacgc tgggcgtcgc cgacatcaac gcgatcgggc cgatctggat
      901 cgtgtcgccg agggatgcag acgcgacccg tgcgctgcgg ccgttctcgg tgttctgggg
      961 gtggacgagg tactcctcgg cggacaggaa cggatcgccg ccgacatctt ccttgaggaa
     1021 gatctccagc cactcgtcga agctgcgctg caacatcgcg tcggccagga tcttgcgtac
     1081 ccgttcgagc tcttcggccg acggcagcag gtcgggcatc gtcgccaggc cgggctcgtc
     1141 cagcaacgac tccaggccga gcacccgcag ccagttgcgg aacaggtgcg gttggcggct
     1201 gcacatctgg atgaaccggt cgtcggcggt gcgcgccgtc atgaacgcga gcggcatgac
     1261 accgaagatg cgaccgctct tgtcgacgtt gtgggtgcga tgaccgaagc cgctgatcat
     1321 gtcgaacgac gccaacgcgt ggagcaggct ggtgtgcagc cgctggccgc gaccggtgcg
     1381 ctcacgctgg aacagcgcgg cgatgatgcc ctgtgtggcg agcatcgccg cgccgtagct
     1441 ggcgattggg gtcggtgtga agatcggtcc gtcgcggaag ccgttcatcg tggccatccg
     1501 cccggtggca gcggcgacga tgtgctcgta gccgggtagg tcgcgcagtg gcccgcgctc
     1561 gccgaagccg gtgatcgcca cctggatcag ccgcgggttg atcgccgaca gccgggcgtg
     1621 atcgatgccg aggcgctcga cggcacccgg cttgaagtcg gtgatcaaca cgtcggcggt
     1681 cgcgatcagg tcgtcgaagg tgttgcgatc gggcgtggtc agatcgacca cgatgctgcg
     1741 cttgccgcgg ttccacacgg caaagccgtc gtgggcgcgg gtggggtcgc cgcccggggc
     1801 ttcgaccttg atcacgtccg acccgttgtc ggcgaacaac atcccggtca tcgctccggc
     1861 catgtggcgg ctggcgtcga cgacgcggag gccctccagt gcagtggtca tgtctacatc
     1921 ccctcgatcg tgcggtcgca ggacctatat gcaggtggtc cggctgtcat ctcaatgatt
     1981 actataatcg gaccctgtga gccctcaata tccatcgtct gggaacattc caccgatctc
     2041 ggaacatcca ccgatcccgg cggtgccccg tcgccccgtg cgcagcttgc tcttcatccc
     2101 cggcaaccgg ccggactgga taggcaaggc cgccgcctct ggcgccgacg cggtcgtgtt
     2161 cgatctggag ggctcgctcc ccgcggccga caaggagagc ggccggcgcg gcgtgcgagc
     2221 ggtgctcgac gctgccgatc caaacggccc cgcgatcttc gtgcgggtca acgacgccca
     2281 cggcggcttg atgccggccg atctcgacgt gctcgtcggg cccaacctgg ccggcgtgtt
     2341 gttgccgcag gtgcgttcgg tcgaggaggt gcgcgacctc gccgaccgcc tcgatgcgct
     2401 cgaagccgag cgcgggctcg agcacgggac cgtggcggtc gacccgctga tggagtcgcc
     2461 gcaggcgctc tatcgcgcct acgagatcgc cacgtgtgcc gaccgcatcg cctacatggg
     2521 ttcgggcatc tcgcggcggg gggacatcgt gcgcacgatg ggctaccgct ggacgcccga
     2581 gggtcacgag accttgtact tccgtagcaa ggtgctgctc gaggtgcgtg cggccggtgt
     2641 cgtcaacccg ttgtcgggca tgtggggcga tgtcgccgac ctcggcggac tccgccgcct
     2701 ggccgatcag acccgcgaca tcggttacga ggggatgatg gtgatccacc ccacccacgt
     2761 gccggtcgtg aacgaggtct tctcggccga cccggccgag ctggagcact tccgccnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnggt cgtggagcga tccgcctgcg cggcgaggtg
     2881 gtcgacgaag cccacgtgtt gaccgcgcgt caagagctgg ctcgggccgc gcgctggggg
     2941 ttgtagcggg gcgtcgcccc gtgctggggt tcgtgaggct ggagttcgtg cgatcacggc
     3001 accctttcat cgaaatcaat acactctcag cggtgaaccg cgctgcgatc gacgactcga
     3061 catcacccga ttccgaccat ctcggt
//
LOCUS       DAORNF010000388         3079 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_23960_length_3079_cov_1.468254, whole genome shotgun sequence.
ACCESSION   DAORNF010000388 DAORNF010000000
VERSION     DAORNF010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3079
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23960_length_3079_cov_1.468254"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            315..>708
                     /locus_tag="PLV68_14340"
     CDS             315..>708
                     /locus_tag="PLV68_14340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02479.1"
                     /translation="MAQPVASTWPTEPRWAPPTEPQPRTSTEPDNSRLPSGTGGSPAP
                     NGRPDRSRRSVLVAATIVVAALAGGGAGYAGATLAGDRAETKTVTVTAAPLSYQPGQV
                     LDVTALVTAMTPSVVTIQTETEVRVRGPF"
     assembly_gap    709..804
                     /estimated_length=96
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            820..>1202
                     /locus_tag="PLV68_14345"
     CDS             820..>1202
                     /locus_tag="PLV68_14345"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HRE02480.1"
                     /translation="MTVTSADGTTSAYDADVVGSDTANDIALIRLRNASGLQEAAIGD
                     SDTVLVGEDVVAMGNALALEGGVSVTKGIVSALDRSIDVESGTLDHLIQTDAAISSGN
                     SGGPLLNAAGEVIGINSAGAASSANV"
     assembly_gap    1203..1212
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1361..>1802
                     /locus_tag="PLV68_14350"
     CDS             1361..>1802
                     /locus_tag="PLV68_14350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02481.1"
                     /translation="MTLRLRLVAGLLVLLTIGLATFGVSTYAVYSRTEHDRLDDQIRN
                     SMPVLAESLFAQVGMTPGFGPSRSVAAPAGSYAQLVAPNGTVLVDATVSLGGQGASSD
                     RPALPTQLLGNGETTRFFTVGSSVGSGDWRVAARRFHVINSDQPF"
     assembly_gap    1803..1838
                     /estimated_length=36
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1839..>2208
                     /locus_tag="PLV68_14355"
     CDS             <1839..>2208
                     /locus_tag="PLV68_14355"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HRE02482.1"
                     /translation="RRFALIEVVAAVALLAALAAGSWLILRRGLRPLEQMAGATRTIA
                     AGDLSVRVEPADGRSEVGQLGMAMNSMLDEIEQSFNERAATEASLRRFLSDASHELRT
                     PLTSIRGFAELYRLNGARDDF"
     assembly_gap    2209..2449
                     /estimated_length=241
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2450..>2798
                     /locus_tag="PLV68_14360"
     CDS             <2450..>2798
                     /locus_tag="PLV68_14360"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HRE02483.1"
                     /translation="VTVTASADLLQQAVANLVANAIAHTPSGTPIEIEATAEGSDAIV
                     RVSDRGPGLSPEALVHAFDRFWQADPARVGSGSGLGLSIVYGIVTGLGGTVTAANRAG
                     RGATFEIRVPVAAG"
     assembly_gap    2799..2810
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2838..>3079)
                     /locus_tag="PLV68_14365"
     CDS             complement(2838..>3079)
                     /locus_tag="PLV68_14365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRE02484.1"
                     /translation="TIELTVTEFKLLRYLMANARRVLTREQILEAVWQYDFGGNAGVL
                     ETYISYLRHKVDTETPHLIHTIRGVGYSLRLPSSG"
BASE COUNT          409 a          925 c          927 g          423 t
ORIGIN      
        1 ggcggtgccg cggcgatggt cgccgccgtc gacggcagcc gcaccaacgt gatcggcctg
       61 ccggtcgagt tgctgaccga catgctggcc agcatcgacc tcacgctgcg cgacctgccg
      121 cagcagcgct gacgcttgcc gggcagaccc agggaactcc cagcgtcacc tgagcctgct
      181 cacaagctgg aggcgcagag tgaggaacaa caccgggtgc tgctcctccc tcggcacccg
      241 ggagatcgcc aaggacacgc cacatggacc ccctccttcc caacaccagc cactggccca
      301 cagcgggtca accgatggcg cagcccgttg ccagcacgtg gccgacagag cctcggtggg
      361 caccacccac cgaaccccaa ccgagaacat caacggaacc ggacaactcc cgcctcccgt
      421 ccggaaccgg gggatcgccc gcccccaacg ggcgtcccga ccgctcccgc cggtctgttc
      481 tcgtcgccgc gacgatcgtc gtcgcggccc tggccggtgg tggcgccgga tacgccggtg
      541 ccaccctggc gggggaccgg gccgagacca agaccgtcac ggtcacggcc gcaccgctgt
      601 cgtaccagcc gggccaggtg ctcgacgtca ccgcgctggt cacggcgatg accccttcgg
      661 tcgtcaccat ccagaccgag accgaggtac gcgttcgcgg tccgttcgnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn nnnngcaaca aagatcacgg tgacggtcac cagcgccgac
      841 ggcacgacct cggcctacga cgccgatgtc gtcgggtcgg acacggccaa cgacatcgcc
      901 ctgatccgcc tgcgcaacgc cagcggcttg caggaggcgg caatcggcga ctccgacacg
      961 gtgctggtcg gcgaggacgt ggtggcgatg ggcaacgcgc tcgcgttgga aggcggcgtg
     1021 tcggtcacca agggcatcgt ctctgccctc gaccgttcga tcgacgtcga gtcgggcacg
     1081 ctcgatcacc tcatccagac cgatgccgcg atcagctccg gcaactcggg cggcccgctg
     1141 ctcaacgcgg ccggtgaggt gatcggcatc aacagcgccg gcgcggccag ctcggccaac
     1201 gtnnnnnnnn nngcacccga aggtcgagag cttcgtgaaa aacagcggcc tgggcttcgc
     1261 gatcccgatc aacgcggcga tggagatcgt cgcgggccta cgcgccggga gctgacgagc
     1321 ggtgccgtga tcggtagagg ttgtgctagc gaatacggcg atgacgctga gattgcggtt
     1381 ggtcgccggg ttgttggtct tgttgaccat cggcctggcg accttcggcg tgtcgaccta
     1441 cgcggtgtat tcacgcaccg agcacgatcg actcgacgac cagattcgca attcgatgcc
     1501 ggtgctggcc gagtcgctgt tcgcgcaggt gggcatgacg ccggggttcg gcccatcgcg
     1561 ttcggtcgcc gcgccggccg ggagctacgc ccagttggtt gctcccaacg gcaccgtgct
     1621 ggtggacgcg actgtgagcc tgggcggcca gggcgcctcc agcgatcggc ccgcgctgcc
     1681 gacccagttg ctcggcaacg gtgagacgac gcggttcttc accgtcggtt cgtcggttgg
     1741 cagcggcgac tggcgggttg cggcgcggcg gttccatgtg atcaacagcg accagccgtt
     1801 cgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncg ccggttcgcc ctgatcgagg
     1861 tggtcgccgc ggtcgcgctg ctggccgcgt tggccgcggg gtcatggctg atcctgcgcc
     1921 gcgggttgcg tccgctggaa cagatggccg gggcgacgcg cacgatcgcc gcgggcgacc
     1981 tgtcggtgcg ggtcgaaccg gccgacggcc gcagcgaggt cggccagttg ggaatggcga
     2041 tgaactcgat gctggacgag atcgagcaga gcttcaacga gcgcgccgcg accgaggcgt
     2101 cgctgcgccg cttcctcagc gacgcgtcgc acgaactgcg caccccgctg acctcgatcc
     2161 gtggctttgc cgagctctac cggttgaacg gcgcgcgcga cgacttcgnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng gtcacggtga
     2461 ccgcgagcgc cgatctgctg caacaggccg tcgccaacct ggtcgccaat gcgatcgcac
     2521 acacgccctc gggcacgccg atcgagatcg aagcaacggc cgaagggtca gacgcgatcg
     2581 tgcgcgtcag cgatcgtgga ccgggcctgt cgcccgaggc gttggttcat gccttcgacc
     2641 gtttctggca ggccgacccg gcccgcgtcg gttccggctc tggtctgggt ttgtcgatcg
     2701 tctacggcat cgtcacggga ctcggcggca cggtgacggc cgccaaccgt gctggccgcg
     2761 gtgcgacgtt cgagatccgt gtcccggtcg ccgcggggnn nnnnnnnnnn cccccgccgg
     2821 cctcacccgc tgctggccta tccgctgctg ggtaggcgca ggctgtagcc gacgccgcgg
     2881 atggtgtgga tcaggtgtgg cgtctcggtg tcgaccttgt gacgcaggta gctgatgtag
     2941 gtctcgagca cccccgcgtt accgccgaag tcgtactgcc agacggcttc gaggatctgc
     3001 tcgcgggtca gcacccggcg ggcgttggcc atgaggtagc gcagcagttt gaactcggtg
     3061 accgtcagct cgatcgtcc
//
LOCUS       DAORNF010000389         3076 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_23990_length_3076_cov_1.757034, whole genome shotgun sequence.
ACCESSION   DAORNF010000389 DAORNF010000000
VERSION     DAORNF010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3076
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23990_length_3076_cov_1.757034"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>421
                     /locus_tag="PLV68_14370"
     CDS             <1..>421
                     /locus_tag="PLV68_14370"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRE02485.1"
                     /translation="LDWLAAQGIDVGGGGESLLATEHRRPAAQSLTTFTTDQVLDWLA
                     AQPTTEPWFAHVSYLRPHPPYSAPGEWSQRYDPATLDERAGPPIAPQPAGERSAFHDA
                     VLGTPLAAAPTDPAALARLRAQYFGNVSHVDHELGRV"
     assembly_gap    422..436
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <437..>725
                     /locus_tag="PLV68_14375"
     CDS             <437..>725
                     /locus_tag="PLV68_14375"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRE02486.1"
                     /translation="SGAWDRTLIIVTADHGEMLGDHGLIQKVGYWEQSHWIVCIVRDP
                     VHPEGHGTVVREFTENVDIVPTICVAIGADVPPQCDGYPLAPLLRGEGSPSG"
     assembly_gap    726..812
                     /estimated_length=87
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <813..1166
                     /locus_tag="PLV68_14380"
     CDS             <813..1166
                     /locus_tag="PLV68_14380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02487.1"
                     /translation="RGLERQNLAVVRTDTHAYVQFGDGRWLCFDLAADPTWRTTTADA
                     ATVLPLAQAMLQWRQEHTDRTLTGMLCERGGIGRHPPPSPSLSPSLSLSPSPSQPSLP
                     SLPVVAGLRSADRPG"
     gene            1170..1994
                     /locus_tag="PLV68_14385"
     CDS             1170..1994
                     /locus_tag="PLV68_14385"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HRE02488.1"
                     /translation="MVLATALALSSAVLHAGWNLFAKRSSDPFLALWSQFLIAGVIGV
                     VVLLVGGGVPTGVWVWAAISATAHIPYVVALGWAYHHGDFSLAYPIARGGGALFAAIG
                     GIAILGDDLRAMSLVAIGVVAFGMWMLAWGAAPGHVLAALTVAVSIGVYTVSDSHAVR
                     EHDAPLYVFAGIAAVGVAASVTALAMGRGRDLIAMARPQWGRASLAAVMTISAYGLVL
                     VAVRHAPVGYVAALRESSVLIAVFVGHRLLRESSGHRRLAAAALILAGLVLLVATR"
     gene            complement(2028..>2384)
                     /locus_tag="PLV68_14390"
     CDS             complement(2028..>2384)
                     /locus_tag="PLV68_14390"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRE02489.1"
                     /translation="HRALDAVGHPEIADAIMAVPDGVDLTRRMFTELASIVRTGPSAK
                     WLAALEAADVPAAPCLTIDQHLADAQTAHNDLYQVNEWPDWPEAGPMRQVRYPAVFST
                     WGKLHSTGGPPALRSP"
     assembly_gap    2385..2397
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2398..3066)
                     /locus_tag="PLV68_14395"
     CDS             complement(<2398..3066)
                     /locus_tag="PLV68_14395"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRE02490.1"
                     /translation="MLADLGAEVIKIEPPRGDPMRRLSRHPAGVNPMWVNSNRGKRSV
                     TLDLKQPEAQAQARDLLASADLMVCNWRRPVAERLGLRDADLVARNPRLIRVYVTGFG
                     PDGPLADAPTYDMIIHGRLGLTEAHGNGTEPADSSFMVDKVSAMLVCQAALAAIAGRE
                     LHGVADRVDVAMLDAAAYGNFPDVMANRTFLDHSPESARNMQPDAVRPLATADGWIVV
                     VPVTA"
BASE COUNT          404 a          997 c         1053 g          507 t
ORIGIN      
        1 ggctcgattg gctggccgcg caagggatcg acgtcggcgg tggtggcgaa tcgctgctgg
       61 ccaccgagca tcggcgaccc gccgcgcaga gcctcaccac gttcaccacc gaccaggttc
      121 tcgactggct cgccgcgcag cccaccaccg agccgtggtt cgcgcatgtc tcgtacctgc
      181 gtccgcaccc gccgtattcg gcaccagggg agtggtcgca gcgctacgac ccagccacgc
      241 tcgacgagcg tgccgggcca ccgatcgcgc cgcagccagc cggcgaacga tccgcgtttc
      301 acgacgctgt gttgggtacc cctcttgcgg cggcgccgac cgaccccgcc gcgctcgccc
      361 ggttgcgggc gcagtacttc ggcaacgtca gccatgtcga tcacgaactg ggccgagtgt
      421 gnnnnnnnnn nnnnnncctc gggtgcgtgg gatcgcacgc tgatcatcgt caccgccgac
      481 cacggcgaga tgctcggcga tcacgggctg atccagaagg tgggctactg ggagcagagc
      541 cactggatcg tctgcatcgt ccgcgatcct gtccacccgg agggccacgg cacggtggtg
      601 cgcgagttca ccgaaaacgt cgacatcgtg ccgacaatct gcgtcgcgat cggtgccgac
      661 gtgccccctc agtgcgacgg ctacccgttg gcgcccttgc tgcggggcga gggttcgccg
      721 tcaggnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncgcggact ggaacgacag aacctcgcgg
      841 tggtgcgcac cgacacgcac gcctacgtcc agttcggtga cgggcgctgg ctgtgcttcg
      901 acctcgctgc cgacccgacg tggcgcacga ccaccgcgga cgcggcaacc gtgctgccgt
      961 tggcgcaggc gatgctccag tggcgccagg agcacaccga ccgcaccctc accggcatgc
     1021 tctgcgaacg gggcggcatc ggccgtcacc caccaccgtc accgtcactg tcaccgtcac
     1081 tgtcactgtc accgtctccg tcacagccgt cgctgccgtc gctgcccgta gtcgccgggc
     1141 ttcgatctgc cgatcggcct gggtgagcgg tggtccttgc cacggccctt gcgctgtcgt
     1201 cggcggtgct tcacgccggg tggaacctgt tcgccaagcg cagcagcgac ccgttcctgg
     1261 cgctgtggag tcagttcctg atcgccggag tcatcggggt cgtggtcctg ctggtgggtg
     1321 gtggcgtgcc gactggtgtc tgggtgtggg ccgcgatttc cgctaccgcg cacatcccgt
     1381 acgtcgtcgc actcggctgg gcctatcacc acggtgactt ctcgttggcg tacccgatcg
     1441 cccgtggcgg cggtgccttg ttcgccgcga tcggtggcat cgcgatcctc ggcgacgacc
     1501 tccgcgccat gtcgctcgtc gcgatcggcg tggtcgcgtt cgggatgtgg atgttggcgt
     1561 ggggtgctgc acccggccac gtgctcgcgg cgctcacggt cgcggtatcg atcggcgtct
     1621 acacggtcag cgacagtcat gccgtgcgtg agcacgacgc gcccctgtac gtattcgctg
     1681 ggatcgcggc ggtcggtgtg gccgcgagcg tgaccgcctt ggcgatggga cgcggccgtg
     1741 acctgatcgc gatggcgcga ccccagtggg gccgcgcctc gcttgctgcc gtgatgacga
     1801 tctcggccta cggtctcgtg ctggtcgcgg tccgtcacgc tcctgtcggc tacgtcgccg
     1861 cgttgcgcga gtcgagcgtg ctgatcgccg tgttcgtggg acatcgcctg ctgcgcgagt
     1921 cgagcggcca ccgccgcttg gccgccgcag cactgatcct cgccggcctg gtcctgctgg
     1981 tcgccacccg ctgactgcgg tgatcgaggg acgaacgggg aaagccgtca gggggatcga
     2041 agggctggcg ggccaccggt cgagtgcagc ttgccccagg tactgaagac ggccggatag
     2101 cgaacctgac gcatcgggcc ggcctcgggc cagtcgggcc actcgttgac ctggtacagg
     2161 tcgttgtgcg cggtctgggc gtcggcgaga tgttggtcga tggtgaggca cggggcggct
     2221 ggcacgtcgg ccgcttccag agcggcgagc cacttggccg acgggccggt gcgtacgatc
     2281 gacgccaact ccgtgaacat ccgccgggtc agatcgaccc cgtccggaac ggccatgatc
     2341 gcgtcggcga tctcggggtg gccgactgca tcaagggcgc gatgnnnnnn nnnnnnnggc
     2401 ggtgactggg acgacgacga tccacccatc ggcggtggcc agcgggcgca ccgcgtcggg
     2461 ctgcatgttg cgcgccgact ccggcgagtg gtcgaggaac gtgcggttgg ccatgacgtc
     2521 ggggaagttg ccgtatgccg cagcgtcgag catcgcgacg tcgacgcgat cggcaacacc
     2581 gtggagctca cggccggcga ttgcggcgag cgccgcctga cagacgagca tcgccgacac
     2641 cttgtcgacc atgaacgagc tgtccgccgg ttcggtgccg ttgccgtggg cctcggtgag
     2701 accgaggcgg ccatggatga tcatgtcgta ggtcggcgcg tcggcgagcg gaccgtcggg
     2761 cccgaagccc gtgacgtaca cgcggatcag ccgggggttg cgggcgacga gatcggcatc
     2821 gcggaggccg agtcgttcgg caaccgggcg acgccagttg cagaccatca gatcggcact
     2881 ggccagcagg tcgcgtgcct gcgcttgcgc ttccggttgc ttgagatcga gtgtcacgct
     2941 gcgcttgccc cggttggagt tgacccacat cgggttgacc cccgccggat ggcggctgag
     3001 acggcgcatc gggtcaccgc gtggtggctc gatcttgatc acctcggccc cgagatcggc
     3061 gagcatcatc gtggcc
//
LOCUS       DAORNF010000390         3076 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_23991_length_3076_cov_1.617014, whole genome shotgun sequence.
ACCESSION   DAORNF010000390 DAORNF010000000
VERSION     DAORNF010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3076
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23991_length_3076_cov_1.617014"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            679..1011
                     /locus_tag="PLV68_14400"
     CDS             679..1011
                     /locus_tag="PLV68_14400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02491.1"
                     /translation="MDLLLDRCCVFGFAQPNRDVGVSGSEPADQQRERVDGERGESND
                     VETAGVEFGGGSADCDELGGFAQHTAGGFDDVFACGCWLDLPAEPEEEFDPEFRFEAP
                     NPIGQRWL"
     gene            complement(1102..>1620)
                     /locus_tag="PLV68_14405"
     CDS             complement(1102..>1620)
                     /locus_tag="PLV68_14405"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HRE02492.1"
                     /translation="LAQAIGRWGNWFNQELFGRPSTLPWAVQIDRVNRPAGYETFSTF
                     QPTFLYESLWNIAVVGVVLLVERRHRLRPGRLFAVYIAGYAVGRFLVERMRIDFAHTI
                     AGLRVNEWTSILAFSVALTVMLTGRRSSQATLDTPSEAYRSLTADPHTSLQASGASDS
                     EQTEEALGDAQA"
     assembly_gap    1621..1819
                     /estimated_length=199
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1820..2014)
                     /locus_tag="PLV68_14410"
     CDS             complement(<1820..2014)
                     /locus_tag="PLV68_14410"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HRE02493.1"
                     /translation="MISILASFPSPPTNGIGIGPLQLHAYGLMIALGVIVAARWADRR
                     WAARTGDTGVVSSLATWAVPA"
     gene            complement(2062..>2267)
                     /locus_tag="PLV68_14415"
     CDS             complement(2062..>2267)
                     /locus_tag="PLV68_14415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02494.1"
                     /translation="SVLAVPGTIVHAALGHIDWTLVAVFAATSVPLSFLGAKVALRTN
                     AARLERIYGAAISALGVAFLIAR"
     assembly_gap    2268..2284
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2285..2792)
                     /locus_tag="PLV68_14420"
     CDS             complement(<2285..2792)
                     /locus_tag="PLV68_14420"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRE02495.1"
                     /translation="MRLLAVAGIGLAIGFLGGLFGKGGSAIATPLLAAVGVPPIVAVA
                     APLPATIPSTLAAGSVYWKAQLIDWRVVRWSVGCGIPATAAGAVATRWIGGTVLVTIT
                     DAILVALGLRFLLARSGHTTELAAEPRTSAWVLAILAITVGLVSGLLANSGGFLLAPL
                     YVSVLRLPL"
     assembly_gap    2833..2862
                     /estimated_length=30
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2893..>3076)
                     /locus_tag="PLV68_14425"
     CDS             complement(2893..>3076)
                     /locus_tag="PLV68_14425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02496.1"
                     /translation="TTSIDWAITLPFVVAAVAGVVSGARIADRLDPKRSLRWFAGLLV
                     AVATYTAIRATANLVT"
BASE COUNT          470 a          828 c         1044 g          488 t
ORIGIN      
        1 cgccgcaatc ctggccggag tcgctcatcg agatcagccc ggacacgatt cagctgggtc
       61 gcagccgtag gagccatcag cgcggcatca gtgccggact gtgaggagag cccgatcacc
      121 tgcctcgctg ctgtggcctg gtcggctctg tggtcgtcgt tgatcgcggc gtctgcacag
      181 cttcgtcgct cggccctggc aggagcgggc aaccggttca ctcagcgtgc cttgtgggcc
      241 ttggcgagct tgatcatcgc cgttgcggcg ggcgtgagga tcccgcgtcg atgcacgatc
      301 ccgattcggc gtttgacgtc cggccggagt cggcgaacga cggcgccctg cgcgcgggcg
      361 tcgcgtgcca gggagcaggg agacgcctgc gccggcgagc acgaggggga ggatcgcttc
      421 gcgatggttg atctcgacca cgatgtgtgg tgcccgctcg ccgtgtgctg cgactcggtc
      481 gatgagtcgg cgtgtggatg tgccttgtgg ggtgacgatc aggggcatcg ctgcgaagtc
      541 gactgcagcg atggcaccgt cgggcagatc ggtttctggc gggcacacgg cgaagacttc
      601 ttggcggaat agttcgatac gggcgaggcc cgatccgccg gtggtcaggt cggtgaagcc
      661 gagttctgcg cggcctgatt ggacttgctg ctcgatcgat gctgcgtctt cgggttcgct
      721 caaccgaatc gtgatgtcgg ggtatcgggt tcggaacctg ccgatcagca gcgcgagcgg
      781 gtcgacggcg agcgtgggga gagcaacgat gtcgagacgg ccggtgtgga gttcggcggc
      841 ggatcggctg actgcgacga gctcggtggc ttcgcgcagc acacggcggg cggcttcgac
      901 gacgtgttcg cctgcgggtg ttggttggac cttccggccg agccggagga agagttcgac
      961 cccgagttcc gcttcgaggc gccgaacccc attggacagc gatggctgtg agaccttggt
     1021 gcgtgctgct gctttcgtga acccgccgca gtcagcgacg gcgacgatgt actcgagctg
     1081 cctgagatcc atagctctac gctatgcctg cgcgtctccc agcgcttcct cggtctgctc
     1141 agagtcgctc gctccactgg cctgcaggga tgtgtggggg tcggcggtga gcgaccggta
     1201 cgcctcggac ggggtgtcca aggtcgcttg agatgaccgg cgtccggtga gcatgacggt
     1261 gagagcgacc gagaatgcca ggatgctggt ccactcgttg acgcgtagtc ctgcgatggt
     1321 gtgcgcgaag tcgatccgca ttcgttccac gaggaatcgt ccgacggcgt acccggcgat
     1381 gtagacggcg aacagtcgtc caggtcggag tcggtgtcga cgctcgacga ggaggacgac
     1441 gccgacgacg gcgatgttcc agagcgactc gtagaggaag gtgggttgga acgtgctgaa
     1501 cgtttcgtat ccggctgggc ggttgacgcg gtcgatctgc acggcccacg ggagggtgga
     1561 ggggcgcccg aacagttctt ggttgaacca gtttccccac cgtccgatcg cttgggcgag
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnng gcgggcaccg cccacgtcgc aagggagctg acgacacccg
     1861 tgtctcctgt gcgggccgcc caacggcggt ctgcccagcg ggcggcgacg atgacgccga
     1921 gagcgatcat cagcccgtag gcgtgcagtt ggagtgggcc gatgccgatt ccgttcgtcg
     1981 gaggactggg gaacgacgcg aggatcgaga tcatgcgtgt cccagtctgg gagggaatgg
     2041 aggcaacggg gacatttcgt gtcatctggc gatcaggaat gcgacgccga gcgcgctgat
     2101 tgctgcgccg tagatgcgtt ccagccgtgc ggcgttggtc cgtagtgcga ccttggcgcc
     2161 gaggaacgac agcggtacgg aggttgctgc gaacacggcg acgagggtcc agtcgatgtg
     2221 gccgagggct gcgtgcacga tcgtgcccgg cacggcaaga acggatgnnn nnnnnnnnnn
     2281 nnnntcagtg gaagtcgcag gacggagaca tacagcggag cgaggaggaa cccgccgctg
     2341 ttcgcgagca ggccggacac gagcccgact gtgatggcca agatcgcgag cacccacgcg
     2401 ctggtgcgtg gttcagcggc gagttcggtg gtgtggccgc ttcgggcgag gaggaagcgc
     2461 aggccgagcg cgaccaggat cgcgtcggtg atcgtgacga gcaccgtgcc tccgatccaa
     2521 cgggtcgcga cggcccccgc agcggtcgcg ggaatcccgc atccgacact ccatcggacg
     2581 acgcgccaat cgatcagttg cgctttccag tagaccgacc cggctgcgag ggtcgatggg
     2641 atcgttgccg gtagcggcgc tgcgaccgcg acgatcggtg gaacaccgac tgcggccagc
     2701 agcggcgtgg cgatcgcaga gccacccttg ccgaacagac ctccgaggaa cccaatggcc
     2761 agcccgatgc cggcgaccgc aagcaacctc acgatgcagc cctggagttc acgatcaatt
     2821 gtccgtcgac cgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncatagcgt tcagctatgg
     2881 caagcggcct cgtcaggtca cgaggttcgc tgtcgcgcgg atagcggtgt aggtggcgac
     2941 tgcgacgagt aggccggcaa accatcgaag actgcgtttc gggtcgaggc ggtcggcgat
     3001 ccgagctccc gagacgacgc cagcgacggc ggcgacgacg aatggcaggg tgatcgccca
     3061 gtcgatcgac gttgtc
//
LOCUS       DAORNF010000391         3074 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_24008_length_3074_cov_1.691289, whole genome shotgun sequence.
ACCESSION   DAORNF010000391 DAORNF010000000
VERSION     DAORNF010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3074
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24008_length_3074_cov_1.691289"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="PLV68_14430"
     CDS             complement(<1..196)
                     /locus_tag="PLV68_14430"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRE02497.1"
                     /translation="MRAVVIVPTYQEAENIERFLTEVRRAIPAAHILVVDDNSPDGTG
                     ALADAKAAALGQIEVLHRPAK"
     assembly_gap    473..561
                     /estimated_length=89
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <562..>1416
                     /locus_tag="PLV68_14435"
     CDS             <562..>1416
                     /locus_tag="PLV68_14435"
                     /inference="COORDINATES: protein motif:HMM:NF023275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-(1->3)-arabinofuranosyltransferase family
                     protein"
                     /protein_id="HRE02498.1"
                     /translation="YSFFDWLGVPDWIAQRLWIATLMFLAGLGARWAAKVLGVGGSQV
                     GGAGVLIAAMCYQLSPYLLPYISRTSTMLLPWVGLGWLVGLTARAAAATGRRNWRWDA
                     LFGLVMISISATNATAIMMIAPAPVLWLLHAAWERTISWRRALIVGAQLGAVSLAVSA
                     WWIAMLSVQGTYGADVLGYSETLEAVSLTSSAPEVVRALGYWLFYVRDPWGFTTTASF
                     DYMASGRLIAISFLVLGIGLCGLVATRWSARRFAVLLVVTGVVLAVGVHPIGDASPLM
                     SPFADNSR"
     assembly_gap    1417..1426
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1701..1715
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1716..>3074
                     /locus_tag="PLV68_14440"
     CDS             <1716..>3074
                     /locus_tag="PLV68_14440"
                     /inference="COORDINATES: protein motif:HMM:NF023275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-(1->3)-arabinofuranosyltransferase family
                     protein"
                     /protein_id="HRE02499.1"
                     /translation="GQEFGAFRWGYTSDPPLPGLTDRPLVTRDLLPLGSAPAMDLLYA
                     FDDRFQDGTAEAASVAAIARLFAADTIWLPNDVAFDRYRTPRPELTAALFAATGADSG
                     LGDAIGVGTPQPNTPVLAMVDEDSLSNPSIGQPLAPVELITVTDPVPIVRAKSEVVML
                     AGSGDGVVDAERWLYWSGTKKLLRYTADASVTADDIAAADRVIVTDSNRDRAHHWHSS
                     QDVWGFTEDGADGQGVLVDSTDDERIPVFSPDEPADRTAADQRGGTRAAASAYGEPFA
                     YRPESRAAMAVDGDLATAWVVADRWNPVGEFITVEADDAFPTVTLVQPHDARNRWITA
                     VTMTTGGDTGADVGAGGEPFTVALDASSHTPQGQVITLPRPSASVTITIAAIEHHPAA
                     SLAGLDAVGFAEVTTAAGPSEEVVRVPSNVLPAVGADQPLDVVLTRLRTRATNRWRAD
                     PE"
BASE COUNT          390 a         1035 c         1050 g          485 t
ORIGIN      
        1 gcttggccgg gcggtggagc acctcgatct ggcccagcgc ggcggccttc gcgtcggcca
       61 gcgcacccgt gccgtcgggg ctgttgtcgt cgaccaccag gatgtgtgcg gccgggatcg
      121 cccggcgcac ctcggtgaga aagcgctcga tgttctcggc ctcttgatag gtggggacga
      181 tcacgacggc gcgcatgcgc agggcagact accgacggca aatcggtcgc gtgttagcgt
      241 gccgctgcac cgagtgctgc catgaccgac gcatccgctc ccctctcctc gcagcagccc
      301 acgcggaccc cagcaagcaa cccagcaagc actgtgcgag cgatggcggc gcgcagttgg
      361 caggtcgtgc tgttggcgct gttggcctac gtgccggcgc tgctgtcgtc gccggggcgg
      421 atgctcggcg acaccaagct ctacctgtac ctcgacccca gccgtctcac cgnnnnnnnn
      481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      541 nnnnnnnnnn nnnnnnnnnn ntctactcgt tcttcgactg gctcggagtg cccgattgga
      601 tcgcccagcg gctgtggatc gcgaccctga tgttcctggc cgggctcggt gcgcgctggg
      661 cggccaaggt gctcggcgtc ggcggcagtc aggtgggcgg cgcgggcgtg ctgatcgcgg
      721 cgatgtgcta ccagctctcg ccctacctgc tgccctacat ctcgcgcaca tccacgatgc
      781 tgctgccctg ggtcgggctg ggctggctgg tcgggttgac cgctcgcgcg gcggccgcga
      841 ccgggcggcg caactggcgc tgggacgcgc tgttcgggtt ggtgatgatc agcatcagcg
      901 ccaccaacgc cacggcgatc atgatgatcg cgcccgcacc cgtgctgtgg ttgctgcacg
      961 cggcgtggga gcgcacgatc tcatggcggc gcgcgctgat cgtcggcgcc caactcggcg
     1021 cggtcagcct ggccgtgtcg gcgtggtgga tcgcgatgct gtcggtgcag ggcacgtatg
     1081 gcgcggacgt actcggctac tccgagacgt tggaagcggt gtcgctgaca tcgtccgcgc
     1141 ccgaggtcgt acgcgccctg ggctactggt tgttctacgt gcgcgacccg tggggcttca
     1201 ccaccaccgc gtcgttcgac tacatggcct ccggccgcct gatcgcgatc agcttcctgg
     1261 tgctcggcat cgggttgtgc ggtctggtcg cgacccgctg gtcggcacgc cgcttcgccg
     1321 tgctgctggt ggtcaccggc gtggtgctgg ctgtcggcgt gcacccgatc ggcgatgcgt
     1381 cgccgctgat gtcaccgttc gcggacaact cccgctnnnn nnnnnngcgc agcagcacgc
     1441 gggcgatgcc gctgagcgtg ctcggcttgg cgctgggcac cggtgcgctg gtcaccgcca
     1501 tcgccggtgc gcgctggtcg cggcggttcc agctgcggcg gttggtaccg atcgggtgcg
     1561 cgttgctggt cgtcgccaac ctgcctgcgc taacccgcgc ggatctggtc gatgccgcga
     1621 tcgaacgcga ccaggacgtg cccgaggcgt ggaccaccgc cgccggctac ctcgacgact
     1681 tgcccgccgg ttatcgggtg nnnnnnnnnn nnnnncgggc caggagttcg gtgcgttccg
     1741 gtggggctac acgtcggacc cgccgctgcc cggtctgacc gatcggccgc tggtcacccg
     1801 cgatctgctg ccgctgggca gcgcgcccgc gatggatctg ctctacgcgt tcgacgaccg
     1861 attccaggac ggcaccgccg aggccgccag cgtcgccgcc atcgcccgcc tgttcgcggc
     1921 cgacaccatc tggctgccca acgacgtcgc cttcgaccgc taccgcacgc cgcgccccga
     1981 gctgaccgcg gcgttgttcg cggcgacggg tgccgacagc gggctggggg acgcgatcgg
     2041 tgtcggcaca ccgcagccga acacgccggt gttggcgatg gtggacgagg actcgctgag
     2101 caacccgtcg atcggccagc cgttggcccc ggtggagctg atcacggtca ccgacccggt
     2161 gccgatcgtg cgtgccaagt ccgaggtggt gatgctggcg gggagcggcg atggcgtggt
     2221 cgacgccgaa cgttggcttt attggagcgg cacaaaaaaa ctgctgcgct acaccgccga
     2281 cgcctcggtc acggccgacg acatcgccgc cgccgaccgg gtgatcgtca ccgactcgaa
     2341 ccgtgaccgc gcccaccact ggcacagctc gcaggacgtg tggggcttca ccgaggacgg
     2401 cgctgacggc caaggggtgc tggtcgacag caccgacgac gaacgcatcc cggtgttttc
     2461 tccggacgaa ccggccgatc gcaccgcggc cgatcagcgc gggggcacgc gcgctgcggc
     2521 cagcgcctac ggcgagccgt ttgcctaccg gcccgagagc cgcgctgcaa tggccgtcga
     2581 cggcgacctg gcgaccgcgt gggtggtcgc cgatcggtgg aacccggtgg gcgagttcat
     2641 caccgtcgag gccgacgacg cgttccccac cgtgacgttg gtgcagccac acgatgcccg
     2701 caaccgctgg atcaccgcgg tcacgatgac gaccggcggg gacacaggcg ctgacgttgg
     2761 ggccggaggt gaaccattca cggttgccct cgacgcgtcg tcgcacaccc cgcagggtca
     2821 ggtgatcacg ttgccccggc cgtcggcgtc ggtcacgatc acgatcgcgg ccatcgaaca
     2881 ccacccggcc gcatcgctcg ccgggctcga tgcggtcggc ttcgccgagg tcacgaccgc
     2941 cgcgggaccg agcgaagagg tggtccgcgt gccgtccaac gtgttgcctg ccgtcggtgc
     3001 cgaccagccg ctcgatgtcg tgttgacccg gctgcgcacc cgtgcgacca accgctggcg
     3061 ggccgacccc gagc
//
LOCUS       DAORNF010000392         3067 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_24087_length_3067_cov_1.897742, whole genome shotgun sequence.
ACCESSION   DAORNF010000392 DAORNF010000000
VERSION     DAORNF010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3067
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24087_length_3067_cov_1.897742"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            15..155
                     /locus_tag="PLV68_14445"
     CDS             15..155
                     /locus_tag="PLV68_14445"
                     /inference="COORDINATES: protein motif:HMM:NF014464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsfS/YbeB/iojap family protein"
                     /protein_id="HRE02500.1"
                     /translation="MEGAREQQWVLIDYGDIVVHVFSDETRAFYEIERLYRDAAQIDW
                     AA"
     gene            221..293
                     /locus_tag="PLV68_14450"
     tRNA            221..293
                     /locus_tag="PLV68_14450"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:254..256,aa:Ala,seq:ggc)
     gene            424..1374
                     /locus_tag="PLV68_14455"
     CDS             424..1374
                     /locus_tag="PLV68_14455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02501.1"
                     /translation="MDAHLTEWLLDSDPALRWQVERDLLGAPEQVWRATRARVTTEGF
                     GAELLAHQGSDGQWAGGAFFPAGWDWDSGEAQPWTATTWVLKDLREWGLDASALTGTA
                     EKLAAARWEYDDLPYWGGEVDCCINSWTLSNGLWLGADVDGIVDWFLEHQLADGGWNC
                     EWVEGATVGSYHSTLNALVGLLDYQLATGDATRVAQARRLGEEYLLCRGLFRRLSTGE
                     AFSEQVFSLAHPRRAYYHALAAADHLRAAAIADGAAPDERMAEVIERVRTQRQPDGSW
                     LQGHRLAGQVWFHVDVPAGEPSKWVTLQALRVLAWWDATR"
     gene            1610..>3067
                     /locus_tag="PLV68_14460"
     CDS             1610..>3067
                     /locus_tag="PLV68_14460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02502.1"
                     /translation="METWAWCGVRPDEPDALAKVTAMARDAGIDATFGPCRGWFPGYS
                     AAFPGDRYVDAATYMQLVRLNASVGMQTVVYDARVWDADPAVRAAAKQFWRPVYEHIA
                     AWDVGDEYFPESDEWQLLIERMQIVLDDVTLDSGVYPYTNHISDAVELALEEVPDQTR
                     LLSFDLYTGDKGVSVARAVADRVERLMCAVNTYQHLIFVPTPASVRADTAALRDAGCD
                     MILVFGGDEVRQQDPSVTFGPESIIDARTAEVTPLGTAMLEAAGHSSYRPVNPARLAD
                     TRGGYSTVDGAFNGIGRRNSATTTELAIAGRAGVGADAASAALTITAVDGAVAGFLTV
                     YPCTDERPNAAQVNYGAGTVVATTMLVGLSENGSVCIYTSADVDLVVDVAGSFPAAAE
                     AFVATAPARLVDTRVDPTLATVDGQYLGIGVRGAGSVSQITVANRADVPWGVSSAALT
                     VTVVGAATAGFVTVFPCGQARPETASVNFAPGSVTT"
BASE COUNT          437 a          973 c         1123 g          534 t
ORIGIN      
        1 gttcgccgtt gcgggtggaa ggtgcccgcg agcagcagtg ggtgctgatc gactacggcg
       61 acatcgtcgt gcatgtgttc agcgacgaga cgcgggcgtt ctacgagatc gagcggctct
      121 atcgcgacgc cgcgcagatc gactgggcag cctgaccgcc aggttgggtg gacagggttg
      181 ggagcgggcg cgccgggcgc tatcttcact ctccggctac ggggctgtag ctcaattggt
      241 agagcgtctc catggcatgg agaaggtcag gggttcgatt cccctcagct ccacccaggt
      301 cagagccgct tttcaccctg gtgaaaggcg gctctttcgc gtccggaagc caggggagac
      361 ccaagattcg gtgggacggc cgccggtcag tcgctgggtc ggctgggagt agcgttggtt
      421 tcggtggatg cgcatctgac cgagtggctc ctggattcgg atcctgcttt gcgctggcag
      481 gtcgagcgtg acctgctggg cgcgccggag caggtgtggc gggcgacccg ggcgcgggtc
      541 accaccgaag ggttcggcgc cgagctgctg gctcatcagg gctcggacgg ccagtgggcg
      601 ggtggtgcct tcttcccggc cggttgggac tgggacagcg gcgaagcgca gccttggacg
      661 gccaccacct gggtgctgaa ggacctccgc gagtggggtc tggacgcctc ggcgttgacc
      721 ggcactgccg aaaagctcgc cgcggcacgc tgggagtacg acgatctgcc gtactggggc
      781 ggcgaggtcg actgttgcat caactcgtgg acgctctcca acggcttgtg gctgggggcc
      841 gatgtcgatg ggatcgtgga ctggtttctc gaacatcaac tcgccgacgg cggatggaac
      901 tgcgagtggg tcgagggcgc gacggtcggg tcctaccact cgacgctgaa tgcgctggtc
      961 gggttgttgg actaccagct cgcgaccggc gacgcgaccc gagtggccca ggcgcgtcgc
     1021 ctcggtgagg agtacctgtt gtgccgcggg ctgttccggc gattgtcgac cggagaggcg
     1081 ttcagtgagc aggtgttcag cttggctcat ccgcgtcggg cctactatca cgcgctggcg
     1141 gcggccgatc acctgcgtgc tgcggccatc gccgacggtg ccgcacccga tgagcggatg
     1201 gccgaggtga tcgaacgggt ccgcacccaa cgccaacccg acggcagctg gctgcaaggt
     1261 catcgcttgg ccggccaggt ctggttccac gtcgacgtcc cggccggtga gccgtccaag
     1321 tgggtgacac tgcaagcctt gcgggtgctc gcttggtggg acgccacccg ctgagccgcc
     1381 gacgtccgtc agcgtgctgc gcttccacac gaaatgactg cgacaatgaa ggcgagcgcg
     1441 tccgtccctc gcgcctgtgt cgtgtgaatg gagcctgtgc gcgtgccatc gaggttgatc
     1501 agtcgtctca cactcgtgct ggtcgggtct ggcgtctgcg tcggtctcgt cgccggtgcc
     1561 gcggcggtcg tggtccaagc gccgcaggcg gtggccgcgg ctggagccga tggagacgtg
     1621 ggcatggtgt ggcgtgcgcc ccgacgagcc tgatgccctg gccaaggtca ccgccatggc
     1681 gcgcgatgca gggatcgacg ccaccttcgg gccgtgtcgc gggtggtttc cgggctactc
     1741 agccgccttt cccggcgacc gctacgtcga cgccgcgacg tacatgcaac tggtgcggct
     1801 caacgcgtcg gtcgggatgc agaccgtcgt ctacgacgca cgggtgtggg acgccgaccc
     1861 ggccgtgcgt gctgcggcga agcagttctg gcgccccgtg tacgagcaca tcgcggcgtg
     1921 ggatgtgggc gacgagtact tcccggagag cgacgagtgg cagctcctga tcgagcggat
     1981 gcagatcgtg ctcgacgacg tgacgcttga ctcgggcgtg tatccgtaca ccaaccacat
     2041 cagcgacgcc gtcgaactgg cgttggagga ggtgcccgac cagacccgat tgctctcgtt
     2101 cgacctgtac accggcgaca aaggtgtctc ggtggcccgc gctgtggccg accgcgtcga
     2161 gcggttgatg tgcgcggtca acacgtacca gcacctcatc ttcgtgccca cgccggcgtc
     2221 ggtgcgggcc gacaccgccg cgctgcgtga cgcggggtgc gacatgatcc tggtgttcgg
     2281 cggcgacgag gtgcgccagc aagatccttc tgtcaccttc ggcccagagt cgatcatcga
     2341 cgcccgcacg gccgaggtca cgccgctggg taccgcgatg ttggaagcgg ctgggcactc
     2401 cagctatcgg ccggtgaacc cggcgcgcct ggccgatacc cgcggtggct actccacggt
     2461 cgacggggcg ttcaacggga tcggacggcg gaactcggcc acgacgaccg agttggcgat
     2521 cgctggacgg gcgggagtcg gcgcggacgc ggcgtcggcg gcactgacga tcactgccgt
     2581 cgacggcgcg gtggcgggat tcctcaccgt gtatccgtgt accgacgagc ggccgaacgc
     2641 ggcgcaggtc aattacgggg ctggcaccgt ggtcgccacc acgatgctgg tcgggttgtc
     2701 agagaacggg tcggtgtgca tctatacgtc cgccgacgtc gatctggtcg tcgacgtcgc
     2761 cggttcgttc ccggccgcgg ccgaggcgtt cgtggccacc gccccggcgc gcctggtcga
     2821 cacccgcgta gaccccacgc tggccaccgt cgacgggcag tacctgggca tcggagtgcg
     2881 cggtgccggt tcggtgagtc agatcacggt ggccaaccgg gccgacgtgc cgtggggcgt
     2941 cagttccgcg gcgttgaccg tcaccgtcgt cggcgccgcg acggccggct tcgtcaccgt
     3001 cttcccgtgc ggtcaggccc ggcccgagac ggcatcggtc aacttcgccc ccggcagcgt
     3061 caccacc
//
LOCUS       DAORNF010000393         3067 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_24090_length_3067_cov_1.665339, whole genome shotgun sequence.
ACCESSION   DAORNF010000393 DAORNF010000000
VERSION     DAORNF010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3067
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24090_length_3067_cov_1.665339"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..778)
                     /locus_tag="PLV68_14465"
     CDS             complement(<1..778)
                     /locus_tag="PLV68_14465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02503.1"
                     /translation="MNVELSRRSVLALGAVPVVAGLPLGSRIAAAASPRPEALNVANA
                     SKFVPVPPARLADTREGTGYQRLDANTIRVEVAGLKGMPDNALAAVLNITAVNTNTSG
                     YITVYPTGSIRPVASTLNYQRAGQIIPNLSTVLLGVGGSVDIFCLTPCDVVVDVNGAY
                     VPVFDAVRDGRFVALPTSYRVLDTRDVGVVGAGNTIAVDIGPAGVPASASAVVVNLTV
                     TESRSSGFWTVYPAGTARPGSSSANTDAANQTRANQAILPI"
     gene            complement(901..1944)
                     /locus_tag="PLV68_14470"
     CDS             complement(901..1944)
                     /locus_tag="PLV68_14470"
                     /EC_number="4.2.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase family protein"
                     /protein_id="HRE02504.1"
                     /translation="MTRPDEGRIVSVPLGDRSYDVIVGYGVRERIGELLPASARRAVV
                     VTQDGLPFGLDLAIDHEVITVGHGEQHKSLATIEMLGERFAAMGLTRNDVVIGLGGGM
                     VTDVAGFAAACWHRGVAVVHVSTTLLGMIDAAVGGKTGVNLAAGKNLVGAFWQPSGVI
                     CDLDALATLPQRELRCGLGEMAKYHFLTGDDLLAMDMVDRVARCVAIKAEIVSSDERE
                     GGRRALLNYGHTLAHALEIETEFSMAHGEAVAVGLIYAAHLARVLGRIDDARVAQHYA
                     VVADAYGLGTSIDRTGRPLDRQRLIALMGKDKKALDGLTFALDSARGVEVVAGVPVDA
                     VEAAFGTMAEYRN"
     gene            complement(1941..>2134)
                     /locus_tag="PLV68_14475"
     CDS             complement(1941..>2134)
                     /locus_tag="PLV68_14475"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HRE02505.1"
                     /translation="RAEPATLAERVRTGVHRPLLDDDPEAALAQMYHDREPLYREVSD
                     AIVTVANRSVADVVEAVLR"
     assembly_gap    2135..2325
                     /estimated_length=191
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2600..2749
                     /estimated_length=150
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2750..>3067)
                     /locus_tag="PLV68_14480"
     CDS             complement(<2750..>3067)
                     /locus_tag="PLV68_14480"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HRE02506.1"
                     /translation="FDAAAEEAMIAEIKAAAKDGDSLGGVAEVVAYGLPVGIGSHVQW
                     DRKLDALLAQALMSIQAVKGVEIGDGFTVAGLRGSVAHDAIRWDAAEGRFRRDTTLAG
                     GVEG"
BASE COUNT          446 a          999 c          886 g          395 t
ORIGIN      
        1 cgatcggcag gatcgcctgg ttggcacggg tctggttggc cgcgtcggtg ttcgcgctgg
       61 acgacccggg acgcgccgtg cccgctgggt acacggtcca gaaaccggac gagcgcgact
      121 cggtgacggt caggttgacc acgaccgcac tcgcgctcgc gggtacaccg gccgggccga
      181 tgtcgaccgc gatcgtgttg cccgcgccga cgacaccgac atcgcgggtg tcgaggactc
      241 ggtacgacgt gggcagcgcc acgaaccggc cgtcacgcac cgcgtcgaag accggcacat
      301 aggcgccgtt gacgtcgacc accacgtcgc agggcgtgag gcagaagatg tcgaccgagc
      361 caccgacacc gagcagcacc gtggacaggt tggggatgat ctgcccggca cgctggtagt
      421 tgagcgtcga cgcgaccggg cggatcgacc cggtgggata gaccgtgatg taaccggacg
      481 tgttggtgtt gaccgccgtg atgttgagca ccgcggccaa cgcattgtcg ggcatcccct
      541 tcagccccgc gacttccaca cggatcgtgt tggcatcgag acgctggtag ccggtgccct
      601 cacgagtgtc ggccagacga gccggcggca ccggaacgaa cttggaggcg ttggcgacgt
      661 tgagcgcctc gggccgcggg gacgctgcgg cagcgatgcg cgatcccaac ggcaggccgg
      721 caacgaccgg caccgcgccc agcgcgagga cgctacggcg cgacaactcg acgttcatcg
      781 cattgcttcc agggtcagcg tcaccgttgc catgatccgt acctgtcgtg tcttcgccag
      841 cgcccgcgcc gcgagcgtct gaaaccctag cgcgagcgcc cggcgaagac ggcgcacccg
      901 ctagtttcga tactccgcca tcgtcccgaa cgctgcctcg acggcatcga caggcacacc
      961 ggcgacgact tcgacgccac gcgctgagtc gagcgcgaac gtcagcccgt cgagggcctt
     1021 cttgtccttg cccatcaacg cgatcaaccg ctggcggtcg agcggccgcc cagttcgatc
     1081 gatgctggtg ccgagcccgt acgcgtcggc aaccacggcg tagtgctgcg cgacccgcgc
     1141 gtcgtcgatg cgaccgagca cccgcgccag gtgcgccgcg tagatcaggc ccaccgccac
     1201 ggcctcacca tgggccatcg agaactcggt ctcgatctcc aacgcgtggg ccaacgtgtg
     1261 gccgtagttg agcagcgccc ggcggccgcc ctcgcgctcg tctgacgaga cgatctcggc
     1321 cttgatcgcg acgcagcgcg cgactcgatc gaccatgtcc atcgccagca ggtcgtcgcc
     1381 cgtgaggaag tggtacttgg ccatctcccc caggccgcag cgcagctcgc gctgtggcag
     1441 ggtggccagc gcgtcgaggt cgcagatcac tcccgacggc tgccagaacg ctccgaccag
     1501 gttcttgccg gccgccaggt tgacaccggt cttgccgccg acggccgcgt cgatcatgcc
     1561 cagcagggtg gtggacacat ggacgacggc gaccccccgg tgccagcacg cggcggcgaa
     1621 gccggccaca tcggtcacca tgccaccacc caggccgatc accacgtcgt tgcgggtcag
     1681 ccccatcgcc gcgaagcgct cgccgagcat ctcgatggtg gccaacgact tgtgctgctc
     1741 gccgtgaccg accgtgatca cctcgtggtc gatcgccagg tcgagcccga acggcagccc
     1801 gtcctgggtc accaccaccg ctcgccgtgc cgacgcaggc aacaactcgc cgatccgctc
     1861 gcgaacgccg taccccacga tcacgtcgta ggaccgatcc cccaacggca ccgaaacgat
     1921 ccgcccttcg tccggccgcg tcaccgtaag accgcctcga ccacgtcggc gacggaacgg
     1981 ttggcaacgg tgacgatcgc gtcggacacc tctcgataca gcggttcacg gtcgtggtac
     2041 atctgggcca gggccgcttc gggatcgtcg tcgagcaggg ggcgatgcac accggtgcgg
     2101 acacgctcgg ccaacgtcgc cggctcggcc cgcannnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngttcg accggttcgc
     2341 tcctcgatca cggcgtcgga gtcgaccagg cgccggtgca gccgcccggc gatgctgcgc
     2401 cccaccgtcg tcttgcccgc gcccatcatg ccgaccaaca ccaggtgccg gtcgccgacc
     2461 gcgtcgacac ccgtggcggg agtcatcgca ccgaggcggc gaaggactcg gcgttgcgca
     2521 cgaactctgc caccgaatcg ccgccgaact tgcgctgagc ttcggccgcc aacacgagcg
     2581 ccaccatcgt ctcggccacn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna ccctcgaccc
     2761 caccggccaa ggtggtgtca cggcggaagc gaccctcggc cgcgtcccac cggatcgcgt
     2821 cgtgggcgac gctgccgcgc agaccggcga cggtgaagcc gtcaccgatc tcgaccccct
     2881 tgaccgcctg gatgctcatg agcgcctggg ccagcaaggc gtcgagcttg cggtcccact
     2941 gcacgtggct gccgatgccg accgggagtc cgtacgcgac gacctcggcc acaccaccca
     3001 acgagtcgcc gtctttggcc gcggccttga tctcggcgat catcgcctct tcggccgcag
     3061 cgtcgaa
//
LOCUS       DAORNF010000394         3060 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_24181_length_3060_cov_1.656905, whole genome shotgun sequence.
ACCESSION   DAORNF010000394 DAORNF010000000
VERSION     DAORNF010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3060
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24181_length_3060_cov_1.656905"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>232)
                     /locus_tag="PLV68_14485"
     CDS             complement(<1..>232)
                     /locus_tag="PLV68_14485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02507.1"
                     /translation="EKAPATTVTATVLRATMNDKATSLVISAGAGAGVADGWKGTIVG
                     LKGVTFRVSNVTERNCRGVIEGITAEQVSGKKV"
     assembly_gap    233..367
                     /estimated_length=135
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<368..>619)
                     /locus_tag="PLV68_14490"
     CDS             complement(<368..>619)
                     /locus_tag="PLV68_14490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02508.1"
                     /translation="EANPQNDEGEFDLGLEVLDPGNRVLVKSDLEDEDARELTKTRSL
                     AGLTAGRYLVHIYLQGRLDNADYEIKVKFSSKSTEVASDF"
     assembly_gap    620..744
                     /estimated_length=125
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            941..1300
                     /locus_tag="PLV68_14495"
     CDS             941..1300
                     /locus_tag="PLV68_14495"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRE02509.1"
                     /translation="MARAWWTLSLVLCCVAAGCSGNVAGGSIDGRRVYENACATCHGE
                     AGTPPPSMKAQLGVKDLRAPEFRSRVDRALVARQVRHGSANKLMPAFAGALTDAQIDA
                     VSAYVVEQLGTGPGKAR"
     gene            1371..>2447
                     /locus_tag="PLV68_14500"
     CDS             1371..>2447
                     /locus_tag="PLV68_14500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02510.1"
                     /translation="MWYPNARVMLGKSAAPWGGFALRYRFFIASASLLLALLASGCPR
                     NISGDLATGEDGKTKGAKEMVLENGEARATGIVTYPGGDRVDWKTLTVPKDKSGTLEF
                     KLTWQPPRPGLQLSFEVFDEFGYPVAAPGRSRRGKTRKAQVEDARGTYLVRIYAQQRG
                     DAGKYKLTASLVEKSAGPQFDPSSLDLPNPPNLASIPDVIVPCDAFSFDIKNPQCRTV
                     CPMTPGAAPPNWPGCAGQCTTPTIDNPACWATMPCPSPPDRRVKSCKFPKCDPANIDP
                     ENPNCDNFELPPVFATVLRTEAVGEGSRIVVSAGKEKGVAAKWIGEVLRGTTDVPLEG
                     GRFTVASVRTRESIGTVSLTPDTLK"
     assembly_gap    2448..2457
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2672..2830
                     /estimated_length=159
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2831..>3060
                     /locus_tag="PLV68_14505"
     CDS             <2831..>3060
                     /locus_tag="PLV68_14505"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HRE02511.1"
                     /translation="VIKILIQCCASLAEAHAIGIIHRDIKPDNVFLLNMPGSPDFVKL
                     LDFSVAKLLQEGDKNSTQAGVVFGTPQYMSPE"
BASE COUNT          437 a          919 c          829 g          446 t
ORIGIN      
        1 gaccttcttg cccgacacct gctccgcggt gatgccctcg atcaccccgc gacagttccg
       61 ctcggtcacg ttgctgaccc gaaacgtcac ccccttgagg ccgacgatgg tgcccttcca
      121 accatcggcc acgcccgcgc ccgcgcccgc gctgatcacc aggctggtcg ccttgtcgtt
      181 catggtcgcg cgcagcacgg tggcggtgac cgtcgtcgcc ggcgccttct ccnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnngaa atccgacgcg acctcggtgg acttcgagga gaacttcacc ttgatctcgt
      421 agtccgcgtt gtcgagacgg ccctgcagat agatgtgtac caggtagcga cccgcggtca
      481 ggccggccaa ggagcgagtc ttggtgagct ctcgagcgtc ctcgtcctct agatcgctct
      541 tgaccagcac ccgattgccc gggtcgagga cctccaggcc caggtcgaac tcgccttcgt
      601 cgttctgcgg gttggcctcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnntggggc ttctccacgg cgtcgccttc gccgacttgt
      781 agccattgtg ctcggggatg gatggaccac aagctgccgc catccaggca accacgaggc
      841 agatcgatag ccgaacgaaa gtgctccctc gcaacatgcc cggaacatag cacgtccccc
      901 gttgccgccg ccggtcgggt gtggcacacg tgggggcaac atggcccgcg catggtggac
      961 cctgagcctg gtgctgtgct gcgtggccgc ggggtgctcc ggcaacgtcg ccggaggctc
     1021 catcgatggg cgccgggtat atgagaacgc ctgcgccacc tgccacggcg aggccggcac
     1081 cccgcctccg tcgatgaagg cccagctcgg cgtcaaagat ctgcgcgcgc cggagtttcg
     1141 ctcccgggtc gaccgagcgc tggtcgcgcg ccaggtccgg cacggctcgg ccaacaagct
     1201 catgccggcg ttcgcgggcg cgctcaccga tgcccagatc gacgcggtgt cggcctacgt
     1261 cgtcgaacag ctcgggaccg gccccgggaa agcgcggtaa cgcaaggact tgcggtacca
     1321 gcagggcctg cgctttcggt gcccttggcc ttgtgacttg ggtttgtctt gtgtggtacc
     1381 ctaacgccag ggttatgctc gggaaatcgg cggcaccctg gggaggattc gctttgcgct
     1441 acaggttctt catcgcctct gcatcgcttc tgctcgcgct tctggcctcc ggctgccctc
     1501 gtaacatctc gggcgatctg gccaccggag aagacggcaa gaccaagggc gccaaggaga
     1561 tggtgctgga aaacggggaa gcgcgcgcca ccggcatcgt cacctatcca ggtggtgatc
     1621 gagtggactg gaagacgctc acggtcccca aagacaagtc cgggacgctc gagttcaagc
     1681 tcacctggca gccgccgcgt cctggtctcc agctctcgtt cgaggtgttc gacgagttcg
     1741 gctacccggt cgcggcgccc ggccgcagcc ggcgcggcaa gacccgcaag gcgcaggtcg
     1801 aagacgcgcg tggcacctac ctcgtccgga tctacgcgca gcagcgcggc gacgccggca
     1861 agtacaagct caccgcttcg ctggtggaga agtcggcggg cccgcagttc gacccgtcct
     1921 cgctcgatct tcccaacccg cccaacctgg cgagcattcc tgacgtcatc gtcccctgcg
     1981 atgcgtttag cttcgacatc aagaacccgc agtgccgcac ggtgtgcccg atgaccccgg
     2041 gcgctgcgcc gcccaactgg ccgggctgcg ccggccaatg caccacgccg accatcgaca
     2101 acccggcgtg ctgggcgacc atgccctgcc cgtcgccgcc cgaccgccgc gtcaagtcgt
     2161 gcaagttccc caagtgcgac ccggccaaca tcgaccccga gaacccgaac tgcgacaact
     2221 tcgagctgcc gccggtgttc gccaccgtgc tgcgcaccga ggcggtgggc gaaggctctc
     2281 gcatcgtggt gtccgctggt aaagagaagg gcgttgccgc caagtggatc ggagaagtgc
     2341 tgcgcggcac caccgatgtc ccgctcgagg gtggtcgctt cacggtggcc agtgtgcgca
     2401 ctcgcgagtc gatcggcacc gtgtccctca ctcccgacac cttgaagnnn nnnnnnncgt
     2461 gcgcctgcgc gcccccaagc cgtgacgcca cggcaagcga gccaaccgtg aaccaggatc
     2521 ccttcgtcgg caaagtcatc gacggtcgct acgagatcca agccagagtt ggcgagggtg
     2581 ggatgggcgt ggtgtatcgc gcgcgccagg tctccatcga tcgggtgatc gcgctcaaga
     2641 tgctcaaccc ccacatggcc gcagacccaa cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn gtgataaaga tcctgatcca gtgctgcgcc tcgctcgccg aggcccacgc
     2881 catcggcatc atccatcgcg acatcaagcc ggacaacgtg ttcttgctga acatgcccgg
     2941 ctcgccggac ttcgtgaagc tgctcgactt ctcggtcgcc aagctgctgc aggagggcga
     3001 caagaacagc acccaggccg gcgtggtgtt cggcacgccg cagtacatgt cgcccgagca
//
LOCUS       DAORNF010000395         3055 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_24231_length_3055_cov_1.734667, whole genome shotgun sequence.
ACCESSION   DAORNF010000395 DAORNF010000000
VERSION     DAORNF010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3055
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24231_length_3055_cov_1.734667"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>257)
                     /locus_tag="PLV68_14510"
     CDS             complement(<1..>257)
                     /locus_tag="PLV68_14510"
                     /inference="COORDINATES: protein motif:HMM:NF020741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TilS substrate-binding domain-containing
                     protein"
                     /protein_id="HRE02512.1"
                     /translation="TRTADVLRAESDLLDDLATAIDPTDARALSDAPRALARRAVRMW
                     LGGSPPVDFATVERVLAVARGDAGACDTVDGRRVRRSRQRL"
     assembly_gap    258..361
                     /estimated_length=104
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<362..>637)
                     /locus_tag="PLV68_14515"
     CDS             complement(<362..>637)
                     /locus_tag="PLV68_14515"
                     /inference="COORDINATES: protein motif:HMM:NF013348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRE02513.1"
                     /translation="VAAVARRWGAAFRAEQVTVHDGPNLEARARAARYAVLPPDVLTG
                     HTADDQAETVLLNLLRGSGARGLGAMTPARHPLLGIRRAETHALCRDL"
     assembly_gap    638..657
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1013..1036
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1082..1357)
                     /gene="rsrA"
                     /locus_tag="PLV68_14520"
     CDS             complement(1082..1357)
                     /gene="rsrA"
                     /locus_tag="PLV68_14520"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycothiol system anti-sigma-R factor"
                     /protein_id="HRE02514.1"
                     /translation="MTEQSDPYCADLVPGVSGCEETLRELERFLDRELTDVDRVSIFG
                     HLDGCPDCLQTYDFHAELKMVIAAKCRNDQMPAGLMDKIERCFGDMS"
     gene            complement(1350..2003)
                     /locus_tag="PLV68_14525"
     CDS             complement(1350..2003)
                     /locus_tag="PLV68_14525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRE02515.1"
                     /translation="MADRNAFAQQAMEYAPQLYSAAMRLTRNSADAEDVVQETYLRAW
                     RSFETFQDGTNLRAWLYRILTNTFINTYRAKQRRPAETGMDDLEDFYLYRRMGSLEAS
                     RGASAEDTMFEFFTDDEVKHALEELPEIFRMPVLLADVEEFSYKEIAEILDIPIGTGM
                     SRLHRGRKQMHKLLYDFAVERGLIATAPPAPALTSDTIPTATAAGHGPSDTGISHHD"
     gene            complement(2014..>2418)
                     /locus_tag="PLV68_14530"
     CDS             complement(2014..>2418)
                     /locus_tag="PLV68_14530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012317866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRE02516.1"
                     /translation="DASQMDALDLLIRDDTWRMSELADALRVDPSTATRTVTRLVNAG
                     WVQRRADDDDGRVVVVQLTDAGREMHQRIDRRRGHVLGHLMSAFSSDERAQLATLMSR
                     FADALDEVVHDLTHNPPSPPASQAAPTSPPQH"
     assembly_gap    2419..2445
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          455 a          932 c         1009 g          484 t
ORIGIN      
        1 acaagcgttg gcggctgcgc cgcacacggc gcccgtcgac cgtgtcgcac gcacccgcgt
       61 caccgcgcgc gaccgccagc acccgttcga ccgtggcgaa gtcgacaggc ggcgagccgc
      121 ccaaccacat ccggacggcc cggcgggcca aggcgcgggg tgcgtcggac agggcgcggg
      181 catcggtggg gtcgatcgct gttgcgagat cgtcgagcag gtcggactcg gcccgcagca
      241 cgtctgccgt gcgggtcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 ngaggtcgcg gcacagcgcg tgggtctcgg cgcggcggat gccgagcagt gggtgccgcg
      421 ctggcgtcat tgcacccagc ccccgtgcgc ccgagccacg caacaggttg agcagcaccg
      481 tctcggcttg atcgtcggcg gtgtgcccgg tcaacacgtc gggcggcagc accgcgtagc
      541 gggcggcgcg ggcgcgtgct tccaggttgg ggccgtcgtg gacggtgacc tgctcggcac
      601 gaaacgccgc tccccaccgc cgggcaacgg cggccacnnn nnnnnnnnnn nnnnnnncag
      661 ccgatcctgg gcgcagaccg tggtcgacgt ggaccgcggt ggtcacgcac ccggcgtgga
      721 cggcgatcgc gagcaacgcc atcgagtcgg cgccaccaga gacggcgcac gtgacgtggt
      781 cgccggggcg cagcgacgga cagcgctgcg tcaggtcggc gagcaggctg gccgtgtcag
      841 tcctcgtcgt tgtcggtggc gctgcgcggc ttgcgaccgt tggggtactt ggccgcggtg
      901 acgttcttga tgtagccgcc gatgagaccg acgactgcag cgccacccgc gaaggtgagc
      961 aggaggccaa gccaccagtg gaagattacg gcgatcacga atgcgactct agnnnnnnnn
     1021 nnnnnnnnnn nnnnnntggg caccatccgc aggtgtcagg ccacttgcgg ctccagggtc
     1081 gctacgacat gtcgccgaag caacgctcga tcttgtccat caacccggcc ggcatctggt
     1141 cgttgcggca cttggccgcg atcaccatct tcagttcggc gtggaagtcg tacgtctgca
     1201 agcagtcggg gcacccgtcg aggtggccga agatcgacac ccggtcgacg tcggtcagtt
     1261 cacggtcgag aaaccgttcg agctcgcgca gcgtctcttc gcagccgctg acgcccggga
     1321 cgagatcggc gcagtacggg tcggattgtt cagtcatggt gggagatgcc cgtgtcgctg
     1381 gggccatggc cggcggccgt agctgtggga atggtgtcgg aggtgagagc gggagccggg
     1441 ggagcggtgg cgatcagccc tcgttcgacg gcgaagtcgt acaacagctt gtgcatctgc
     1501 tttcttcccc ggtggagacg gctcatcccc gtgccgatcg gtatgtcgag tatctcggcg
     1561 atttccttgt aggaaaactc ctccacgtcg gccagcaaca ccggcatgcg gaagatctcc
     1621 ggcagttctt cgagcgcgtg cttcacctcg tcgtcggtga agaattcgaa catcgtgtct
     1681 tccgcgctgg cgccgcgcga cgcctccagc gaccccatcc gccggtacag gtagaagtct
     1741 tcgaggtcgt ccatgcccgt ctcggcaggc cggcgctgct tggcccggta ggtgttgatg
     1801 aaggtgttgg tcaggatgcg gtagagccag gcgcgcaggt tggtgccgtc ctggaacgtc
     1861 tcgaagctgc gccacgcccg caggtacgtc tcctgcacca cgtcttccgc gtcggccgag
     1921 ttgcgggtga ggcgcatcgc cgccgagtac aactgcggcg catactccat cgcctgctgc
     1981 gcgaacgcgt tgcggtcagc catcggcggc aatctagtgc tgtggcggcg acgttggggc
     2041 tgcctgggac gccggtgggg acggcgggtt gtgggtgagg tcgtggacga cctcgtcgag
     2101 ggcatcggcg aagcgggaca tcagcgtggc caactgggcg cgctcgtcgg agctgaacgc
     2161 gctcatcaga tggccgagca cgtggccgcg gcggcggtcg atgcgctggt gcatctcgcg
     2221 cccggcgtcg gtgagctgca cgaccaccac acggccgtcg tcgtcgtcgg cacgccgctg
     2281 gacccacccg gcgttgacca ggcgggtcac ggtgcgcgtc gccgtggacg gatcgacccg
     2341 caacgcgtcg gccaactcgc tcatccgcca cgtgtcgtcg cggatcagca ggtcgagcgc
     2401 gtccatctgg ctcgcgtcnn nnnnnnnnnn nnnnnnnnnn nnnnntcacc gaacaggcga
     2461 tgctcagcga ccccgccagc gccgcggcgc agcttgcgcc aggcgacccc caagccgacg
     2521 gcgacgtcga ggtcttcgcg gcgactgaag tcgacgcgaa agtgtccggt gacccgccca
     2581 tccgtcctgg ctgcatcggc cgatgccatg gggcgcaccg tagtgagcgg cttaccgcgg
     2641 ttgcgaaccg gattccagac agttttcaga tcgaaccact tgtgtcaggt acgcctcatg
     2701 tggttacatt ggtgccgttc ttgttgtcga gggggaacga tgtacatctg cagctgccga
     2761 gccgtcagtg atcgcaccat tcgggccacg attgctgctg gcgcgggctg catcgacgac
     2821 gtcgatttca ttatcggcac cttctccacc ccgaactcga gcgcctgctt gccgagtcgg
     2881 tgaaggtcgg caagactcga ccgggtcagg ctgcttgacc cacacgcgtc gcctggcaag
     2941 gagcaggcca tgcaaggcaa cgacgagatc atcgagctac tcaacgaggt gctgaccgcc
     3001 gagttgaccg ccatcaacca atacttcgtc gacgcgaaga tgttcgccaa ctggg
//
LOCUS       DAORNF010000396         3045 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_24352_length_3045_cov_1.455853, whole genome shotgun sequence.
ACCESSION   DAORNF010000396 DAORNF010000000
VERSION     DAORNF010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3045
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24352_length_3045_cov_1.455853"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="PLV68_14535"
     CDS             <1..240
                     /locus_tag="PLV68_14535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02517.1"
                     /translation="LSTMGYALPGSVIAAGVLRIVKELADSGVTVLLVEEKPQQVLAI
                     ADRVALLRLGHVVWEGPRDELDEARIEDVYLGRAS"
     gene            complement(244..1035)
                     /locus_tag="PLV68_14540"
     CDS             complement(244..1035)
                     /locus_tag="PLV68_14540"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRE02518.1"
                     /translation="MDQSGVGADAGVPVALATRGVSVQFGGVRALDDVTISIGRGEVC
                     GLIGPNGAGKTTLFDVLSGVRSPKLGRIELHGEDVTDKSATWRARNGMRRTFQRQQVF
                     GGLTVEDNVLTALEWEGGGGGFLADMIASPARRRRERNRRAQVRNVLSTFRVDSMRDD
                     AAGTVPIGAARELEVARAIVARPSVLLLDEPTSGLDESERDRLGEIIQAERSRGTAVV
                     LVEHDVEFVMAQCDRIVVLNLGEVIADDVPAEIRKNPAVLAAYLS"
     assembly_gap    1135..1158
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1290..>1504
                     /locus_tag="PLV68_14545"
     CDS             1290..>1504
                     /locus_tag="PLV68_14545"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02519.1"
                     /translation="MTRRGARCAIGYATNEANAAETAVRTAEVGAGAVLVKGDIATEG
                     PAIAASAIAQLGGLDAIVVTAGVETLGR"
     assembly_gap    1505..1559
                     /estimated_length=55
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1560..1974
                     /locus_tag="PLV68_14550"
     CDS             <1560..1974
                     /locus_tag="PLV68_14550"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRE02520.1"
                     /translation="QVWGLLEVVRAATPALAESGGSVVAVSGITADHYAKYYGPIGPS
                     KGALETLVKYLAAELGPRGIRVNAISPCLIADDDHWKPDAVAPFWEEVRKRTPLRRLA
                     TPDDIAGSIIALLGSDLGYVTGNVMIVDGGYTLLS"
     assembly_gap    1988..2107
                     /estimated_length=120
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2108..2744)
                     /locus_tag="PLV68_14555"
     CDS             complement(<2108..2744)
                     /locus_tag="PLV68_14555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HRE02521.1"
                     /translation="MSFTESERAAADKLLTTTRSVRRRLDLERPVPRSLVMECIEMAL
                     QAPTASNLQTWRFVVVTDPAQITALGDIFRKTYAAADAPMEYVEANRGQYERIDADQM
                     TEVLSSAQHLISVIDKMPMIVIPCVETRLEEPIQRALAASLYGSIFPAVWSLQLALRS
                     RGLGSSLTTSHIWFAHEVAEVLGIPDGVEQAALIPVAYFTGETFHPAKRLPA"
BASE COUNT          488 a          947 c          939 g          472 t
ORIGIN      
        1 ctgagcacga tgggctacgc attaccgggg tcggtcatcg ccgccggggt gctgcggatc
       61 gtcaaggaac tggccgacag tggtgtcacc gtgctgctgg tggaggagaa acctcaacag
      121 gtgctagcca tcgccgatcg tgtcgccctg ctccgcctcg ggcacgtggt ctgggaaggc
      181 ccccgtgacg agctcgacga ggctcgcatc gaggacgtgt acctgggccg ggcgtcgtag
      241 gcgtcaactc aggtaggcgg cgaggaccgc ggggttcttg cggatctcgg cggggacgtc
      301 gtcggcgatg acctcgccaa ggttgagcac cacgatgcgg tcgcactgcg ccatcacgaa
      361 ctcgacgtcg tgttcgacga gcaccaccgc ggtgccgcgg ctgcgttcgg cctggatgat
      421 ctctccaagc cggtcgcgct cggactcgtc cagtcccgag gttggctcgt cgagcagcag
      481 caccgaagga cgtgcgacga tcgcccgggc gacttccagt tcgcgtgccg cgccgatcgg
      541 cacggttccg gctgcatcgt cacgcatgga gtcgacgcga aacgtcgaca gcacgttccg
      601 aacctgtgcc cggcgattgc gctcacggcg gcggcgggcc ggggaggcga tcatgtcggc
      661 gagaaacccg ccgccacccc cttcccactc gagtgccgtg agcacgttgt cctccaccgt
      721 cagcccaccg aaaacctgct ggcgttgaaa ggtgcggcgc atgccgttgc gggcgcgcca
      781 cgtcgccgac ttgtcggtca catcctcccc gtgcagttcg atacgtccca gcttgggcga
      841 acgaacgccg gacagcacgt cgaacaacgt cgtcttgccc gctccgttcg gtccgatcag
      901 accgcagacc tcaccgcgac cgatgctgat cgtgacgtcg tcgagcgcgc gcacaccgcc
      961 gaactgcacc gacactcccc gcgttgccag cgcaacgggt acaccggcgt ctgcaccgac
     1021 ccctgactga tccacacgcg accccctcgt ccgtcaacga cgcctcgaac ctagcaaacc
     1081 gaacgattgg tttctacttg ccgacgatcg tccggctgag cttcactaga gcacnnnnnn
     1141 nnnnnnnnnn nnnnnnnnct gtctggacgg cagtcttgat cggagccggc gacatggaca
     1201 gcagcgcagc acgtgatctg aaccgaatgc ctgcgaaggt gctcgtgatc ggaggctcgc
     1261 acggcatcgg acgagcgacg gtcatgcggt tgacccgtcg cggcgcgcgg tgtgccatcg
     1321 gctatgcgac caacgaggcg aacgcagccg agaccgctgt ccgcaccgcc gaggtggggg
     1381 cgggcgcggt actcgtgaag ggcgacatcg caaccgaggg gccggccatt gccgccagcg
     1441 cgatcgcaca actcggtggg ctcgacgcga tcgtcgtcac ggctggggtc gagacgctcg
     1501 gccgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng
     1561 caggtgtggg gcttgctcga agttgtccgt gcggccaccc ccgcactcgc cgaatccggg
     1621 ggttcggtgg tggccgtctc ggggatcacg gccgatcact acgccaagta ctacggaccg
     1681 atcggaccct cgaagggggc gttggagacg ttggtcaagt acctcgccgc cgagctcggc
     1741 ccacgcggga tccgggtcaa cgcgatttcc ccgtgtctca tcgccgatga cgaccattgg
     1801 aagcccgatg ccgtcgcccc gttctgggag gaggtgcgca agcgcacgcc actccgccgc
     1861 ctggcgacac ccgacgacat cgccggcagc atcatcgcct tgctcggttc cgaccttggc
     1921 tacgtcaccg gcaacgtcat gatcgtcgac ggcggctaca cgctgctctc gtgacgtcgt
     1981 ccgtgatnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnncgg ccggcaggcg cttcgctgga tggaacgtct caccggtgaa gtaggccacc
     2161 ggtatcagcg cggcctgctc gaccccgtcc ggaataccga gcacctcggc gacctcgtga
     2221 gcgaaccaga tgtgcgaggt ggtcagcgag gagcccagac cacggctgcg caacgccagt
     2281 tgcagtgacc agacggccgg aaagatcgag ccgtacagcg acgcggccaa cgcgcgctgg
     2341 atcggttcct cgagacgggt ctcgacgcac gggatgacga tcatcggcat cttgtcgatc
     2401 accgagatga ggtgctgggc ggacgagagc acctcggtca tctgatcggc gtcgatgcgc
     2461 tcgtactggc cacggttggc ttcgacgtac tccatcggcg cgtcggcggc cgcgtacgtc
     2521 ttgcggaaga tgtcgccgag ggctgtgatc tgcgccgggt cggtcacgac gacgaaccgc
     2581 cacgtctgca ggttggaagc ggtcggtgcc tggagcgcca tctcgatgca ctccatcacc
     2641 aagctgcgcg gtactggacg ctcgagatcg agccggcgac gcaccgatcg ggtggtcgtc
     2701 agcagcttgt cggcagccgc ccgctcggac tctgtaaaac tcatgatccc ctcgattcac
     2761 aattcgtgat gtgccatacc tgggccgcgg tggttccccg gccctctaga tgacctacca
     2821 aacgtctgtt aaacttagcc gttgagacga atcgtcagac cgatcagggt ccggcgcgac
     2881 gaccgtccga ggaaggtgcc gagagatgcc gagctctgaa gccgacgcgc tgatcgagat
     2941 gtacgcccaa taccgggtgc ctgacaacgc cccgggtcgc actcccgacg agttgcgagc
     3001 gatggtggct caggctgcag agctggcgag ccccgtcggc gtcgt
//
LOCUS       DAORNF010000397         3043 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_24371_length_3043_cov_1.903614, whole genome shotgun sequence.
ACCESSION   DAORNF010000397 DAORNF010000000
VERSION     DAORNF010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3043
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24371_length_3043_cov_1.903614"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="PLV68_14560"
     CDS             <1..279
                     /locus_tag="PLV68_14560"
                     /inference="COORDINATES: protein motif:HMM:NF014447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02522.1"
                     /translation="PDPWPRGRHARKRLMTPERLVVIVDALAVGGRLRFATDHAAYAE
                     QAMEVIAAEPRLCGGVVPRPAERPITVFEARGLAEGRTPTDIEATRLT"
     gene            complement(410..485)
                     /locus_tag="PLV68_14565"
     tRNA            complement(410..485)
                     /locus_tag="PLV68_14565"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(450..452),aa:Phe,seq:gaa)
     gene            complement(605..680)
                     /locus_tag="PLV68_14570"
     tRNA            complement(605..680)
                     /locus_tag="PLV68_14570"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(645..647),aa:Asp,seq:gtc)
     gene            complement(700..772)
                     /locus_tag="PLV68_14575"
     tRNA            complement(700..772)
                     /locus_tag="PLV68_14575"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(736..738),aa:Glu,seq:ttc)
     gene            1073..1897
                     /locus_tag="PLV68_14580"
     CDS             1073..1897
                     /locus_tag="PLV68_14580"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HRE02523.1"
                     /translation="MTGAPMRIGLCTDSNAQLPPGLADRFGVEIVPLTVIIDEHEYLE
                     GVDLDADAYYAMWDAGIVPPTRVANPSAGQFALAYEQLADRGCTQILSIHTALEGCSI
                     VTAARLAAHTSPIPVRLIDSGTARFGVSCCVWAASDALGAGATLEEAAAIAESLAPAV
                     GSVFVAGEGLRSKAAGGGWPRPVIAIYDRGCETIAEAESTVDAVNAMARYVLDWGQHL
                     NVAIGAAHLDTIPIAEALGSAIGESANVREVVHYRLGPSVGAELGPGAVGCVMYPA"
     gene            complement(1928..2518)
                     /locus_tag="PLV68_14585"
     CDS             complement(1928..2518)
                     /locus_tag="PLV68_14585"
                     /inference="COORDINATES: protein motif:HMM:NF020989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2017 family protein"
                     /protein_id="HRE02524.1"
                     /translation="MSVRLAPVTRREGTIGSYDLNLDVEERVLVASLLNDLRSLLAAP
                     DADPDGRLRRLFPSAYHQERDQAEDAEFRRFMRDDLVASHLTSIERIHAVLAPANDPS
                     GPTSSGDGLSGGERSGRLRRRKPVEPAPVVLDGAHVESLVQCVNGIRLVLGTMLDVSE
                     DDPDEIDDDDPLIGERYLYDFLSWILDSAIRALQGV"
     gene            complement(2535..2846)
                     /gene="clpS"
                     /locus_tag="PLV68_14590"
     CDS             complement(2535..2846)
                     /gene="clpS"
                     /locus_tag="PLV68_14590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HRE02525.1"
                     /translation="MSLPLASSPAPIVLDRPEEDTFVANDTPWIVLVWNDPINLMSYV
                     TWVFQKLFGYNLDQATKLMMDVHEKGRAVVSNGTREKAELDVFRLHEHGLWATLQRDD
                     A"
BASE COUNT          492 a         1018 c         1037 g          496 t
ORIGIN      
        1 cccgacccct ggccgcgggg ccgccacgcc cgcaagcgcc tgatgactcc tgaacgactg
       61 gtggtgatcg tcgatgccct cgcggtcggt ggccgcctcc gcttcgcgac cgaccacgcc
      121 gcttacgccg agcaggccat ggaggtgatc gccgccgagc cccgcctctg cggtggtgtc
      181 gtccctcgtc cggccgaacg ccccatcacc gtgttcgaag ccaggggcct cgccgaaggt
      241 cgcaccccca ccgacatcga agccactcgt cttacctgaa cgggcctcac ccggaacgag
      301 tcggtgcgac agggaaggga gcgaggtgtt gcgtccggtg atcgacccgc aacggtggtt
      361 ctgggaagac gaaagcccct gccgatctcg tgatcgacca ggggcttcgt ggtggttctg
      421 cccggcgtcg atccggggac cctacgcttt tcaggcgtat gctctgccga ctgagctaca
      481 gaaccaaatg tgctgaaaag gcttgctgac cagcacctgg agcatgtgct ccagagcggt
      541 cccgacgggg ttcgagcccg gcccgacggg cggtgagccc gccgggtggt tccggtatca
      601 gtggtggcgg tcccgacggg atttgaaccc gcgacctccg gcttgacagg ccggcgtgaa
      661 ctccacttca ccacgggacc aggttgttga tctggccagc acccccaacg ggattcgaac
      721 ccgtgccgcc accttgaaag ggtggtgtcc taggccacta gacgatgggg gctaggtacg
      781 acctcggctc cagttgagag cggaggaaga ttatcagtga agcgccacaa accgacgcga
      841 ctttcaccga agttcgccga caacgcagcg ttacagaatc gcaacagcct tgtgtcactc
      901 tgcgatcgga ccgcgacacg tgcctacggt gtggctccat gggacggacc gccacctcag
      961 tcccgtgcgc gtccgtgcac gggttgcggg cgacgcaggg tgctggcacg acggccaggc
     1021 cagaacgcgc ggggccagca ggcgtgcaga tcgatcacac gggccgcgtc gcatgaccgg
     1081 cgctccgatg cggatcggcc tgtgcaccga cagcaacgcg cagttgccac cggggctcgc
     1141 ggaccgtttc ggagtcgaga tcgtgccgtt gaccgtcatc atcgacgagc acgagtacct
     1201 ggaaggggtc gatctcgatg ccgatgccta ctacgccatg tgggatgcgg gcatcgtgcc
     1261 gccgacgcgg gtggccaacc ccagcgccgg acagttcgcg ctggcctacg agcaactcgc
     1321 tgatcgcggc tgcacccaga tcctgtcgat ccacaccgcg ctcgaaggat gttcgatcgt
     1381 cacagcggcc cgtctcgcgg cccacacctc gccgataccc gtacgcctca tcgactccgg
     1441 cacggcccgc ttcggggtga gctgttgcgt gtgggccgct tctgacgcgc tcggtgctgg
     1501 agccacgctc gaggaggccg cggcgatcgc cgaatcgctc gccccagcgg tgggcagcgt
     1561 gttcgtggct ggcgagggtc tccggagcaa agctgctggc gggggctggc cgcggccggt
     1621 gatcgcgatc tacgacagag gatgcgagac catcgccgag gccgaatcga ccgtggacgc
     1681 cgtcaacgcc atggcgcgct acgtgctcga ttggggtcag cacctcaacg tcgccatcgg
     1741 agcagcccac ctcgacacca tccccatcgc cgaggcgctc ggctcggcga tcggagagtc
     1801 ggccaacgtg cgcgaggtcg tgcactaccg gctcggcccc agcgttggcg ccgagctcgg
     1861 tccgggtgcc gtcggctgcg tgatgtatcc ggcctgacgc gaccgttctg cttgacgcgg
     1921 cgaggcgtca cacgccttgg agcgcccgga tcgcgctgtc gaggatccag ctcaagaagt
     1981 cgtacagata gcgctcgccg atcagcgggt cgtcgtcgtc gatctcgtcc gggtcgtcct
     2041 cggacacgtc gagcatggtg cccagcacca gccggatgcc gttgacgcac tgcaccagcg
     2101 actcgacgtg tgcgccgtcg agcacgacgg gtgcgggctc gacgggcttg cgccgccgca
     2161 accggccgct gcgttcgccg ccggaaagcc cgtcaccgga actggtgggg ccggacggat
     2221 cgtttgccgg cgcgagcacg gcgtggatgc gttcgatgct ggtcaggtgt gaggccacca
     2281 ggtcgtcgcg catgaagcgg cggaactcgg cgtcttcggc ctgatcgcgt tcttggtggt
     2341 aggcggaggg gaacagacgc cgcagccgcc cgtccgggtc ggcgtcgggg gccgccaaca
     2401 ggctgcgcag atcgttcagc aggcttgcga ccaggacccg ctcttcgacg tcgaggttga
     2461 ggtcgtagct gccgatcgtt ccctcccgcc gcgtgacggg agcgagccgg acactcacga
     2521 cgctgatccg gcgctcatgc gtcgtctcgt tgcagcgtgg cccagaggcc gtgttcatgg
     2581 aggcggaaga cgtcgagttc ggccttctcg cgggtgccgt tggacacgac cgcacgaccc
     2641 ttctcgtgga cgtccatcat cagcttggtg gcctggtcca ggttgtagcc gaacagcttc
     2701 tggaacaccc aggtgacgta gctcatgagg ttgatcgggt cgttccagac cagcacgatc
     2761 cacggggtgt cgttggcgac gaaggtgtcc tcctcgggcc ggtcgagcac gatcggcgcg
     2821 gggctggacg cgaggggcag agacatcgac cccattctcg ccccatttcg cccgctgcca
     2881 ccacaacgcc cacccacaac gtgatgtcac gggcggggtg ctggcaccac gatcacgggg
     2941 cagggggcgt tgtcgcacag cgcgatcgcc gtcgaaccgg agatcaaggt cgagagcccg
     3001 gcccgtcccc gggtaccgac cacgatcacg ctggcgtcgg cag
//
LOCUS       DAORNF010000398         3038 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_24415_length_3038_cov_1.434462, whole genome shotgun sequence.
ACCESSION   DAORNF010000398 DAORNF010000000
VERSION     DAORNF010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3038
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24415_length_3038_cov_1.434462"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>347
                     /locus_tag="PLV68_14595"
     CDS             <1..>347
                     /locus_tag="PLV68_14595"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRE02526.1"
                     /translation="TKAKAKRAMRDMADELLKLYAERKLVQGYAFSPDAPWQHEFEDA
                     FPYDLTVDQAKAIEDVKGDMETPQPMDRLIIGDVGYGKTEVAMRAAFKAVMDGKQVAV
                     LTPTTILAYQHFE"
     assembly_gap    348..523
                     /estimated_length=176
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <524..>738
                     /locus_tag="PLV68_14600"
     CDS             <524..>738
                     /locus_tag="PLV68_14600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02527.1"
                     /translation="DEEQRFGVAHKEKLKQLKKKVDVLTLSATPIPRTLNMSMLGMRD
                     MSVIETPPRDRLAINTQVVQFSEGVIRS"
     assembly_gap    739..834
                     /estimated_length=96
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            871..>1085
                     /locus_tag="PLV68_14605"
     CDS             871..>1085
                     /locus_tag="PLV68_14605"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HRE02528.1"
                     /translation="MNEKEMEQVMLDFIDYKYDVLVATTIIENGIDIPRANTIIINRS
                     DNYGLSQLYQLRGRVGRSNRRAYAYLL"
     assembly_gap    1086..1256
                     /estimated_length=171
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1382..>1676)
                     /locus_tag="PLV68_14610"
     CDS             complement(1382..>1676)
                     /locus_tag="PLV68_14610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02529.1"
                     /translation="AVEPPRLARQLLELAVVDLSAGSDLVLSALVPERYQVTAVESDD
                     RALLHWEQHRGGVSISRIDRSPSDLAGVYRLQLQPAAEAIRLYVRNRWLASTI"
     assembly_gap    1677..1686
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1687..2739)
                     /locus_tag="PLV68_14615"
     CDS             complement(<1687..2739)
                     /locus_tag="PLV68_14615"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HRE02530.1"
                     /translation="MTVPDWWVERRYGMRLHASVATVPSFSPIGEAAEYYWAHLGTDA
                     LAGIAPHPSPMAEVLAYHRDRWSHVARYDDFVPFLSYHRFDADEHVELALDAGMNLLV
                     QDVKQHDGFCWWDAPGTTRSSVHAGPRRDVVGEIVTAARRAGMVVGAGYSLFDWAEHR
                     PDRRFATVGDELADLIERYGVELLWGDGADALAPLPPSARNAIADALDRARELAELQG
                     IELALNDGWTGDGTVDGFAVFGPTPPPGIVTAPWVLRRPLGASACFNRAERDEHLLGP
                     GALVDLLTEVVAKGGTMVVDVGPGFDGSIPDTIARTLRDVGTWVYDHADVVHGSRPFD
                     VWGDARFRYVQVPAEAG"
BASE COUNT          499 a          809 c          843 g          434 t
ORIGIN      
        1 gaccaaggca aaggccaagc gcgcgatgcg cgacatggcc gacgaactac tcaagctcta
       61 cgccgaacga aagctcgttc agggttatgc gttttcgccg gatgcgccgt ggcagcacga
      121 atttgaggac gcgttccctt acgacctgac cgtcgatcag gcaaaagcga tcgaagatgt
      181 aaaaggcgat atggaaacgc cgcaaccgat ggaccgcctg ataattggcg acgtcggtta
      241 cgggaagacc gaggtggcga tgcgtgcggc gttcaaggcg gtgatggacg gcaaacaggt
      301 agccgtcctt acaccgacta ccatccttgc atatcagcac tttgaatnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngatgagg agcagcgttt
      541 cggggtcgcc cataaagaaa aactcaagca gctgaagaag aaggttgacg tactcactct
      601 ctcggcaacg cctattccgc gaacgctcaa tatgtcaatg ctcggtatgc gggacatgtc
      661 cgtgatcgaa acgcctccgc gtgatcgcct cgcgatcaat acgcaggtcg ttcagttttc
      721 tgagggggtt atccggtcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngtcccg
      841 aacgcgcgga tcgcgatcgg ccacggaaag atgaacgaaa aggaaatgga gcaggtaatg
      901 ctcgatttca ttgactacaa atacgacgtt ctcgtagcca caacgataat cgaaaacggc
      961 atcgacattc cgcgtgcgaa cacgatcata atcaatcgtt ccgacaatta cgggctgtca
     1021 cagctatacc agcttcgcgg ccgcgtcggc cgttctaatc gtcgtgctta tgcatatttg
     1081 ttgatnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngctc
     1261 taggattcga cctttatacg aaaatgctcg aacgcacgat cgccgaactt cgcggtgacg
     1321 agatcgccga cgagatcagc gtttcgataa acctcggtat cgatgtctcg ataccaaaag
     1381 actatatcgt tgaggcaagc cagcgattgc gaacgtacaa gcggatcgcc tcggccgcgg
     1441 gctgcagttg cagccggtag accccggcca ggtcggacgg ggagcggtcg atgcgggaga
     1501 tgctgacacc gccgcggtgc tgctcccagt gcagcagggc tctgtcgtca ctctcgacgg
     1561 cggtgacctg ataccgctcg ggcaccagtg cgctcaacac caggtcggac ccggcggaca
     1621 ggtcgacgac ggcgagctcg agcagctgac gggcaagccg gggtggttcg acagcannnn
     1681 nnnnnngccg gcttcggctg gcacctgcac gtaacggaac cgggcgtcac cccacacatc
     1741 gaacggacgg ctgccgtgga cgacgtcggc atggtcgtac acccaggtgc cgacgtcgcg
     1801 cagggtgcgc gcgatcgtgt cggggatcga gccgtcgaag ccgggaccga cgtcgaccac
     1861 catggtgccg cctttggcga ccacctcggt gagcaggtcg accagcgcac cggggccgag
     1921 caagtgctcg tcgcgctcgg cgcggttgaa gcaggccgag gcgccgaggg ggcggcgcag
     1981 cacccacggc gcggtgacga tgcccggcgg gggagtaggc ccgaacacgg cgaagccgtc
     2041 gacggtgccg tccccggtcc atccgtcgtt gagggcgagc tcgatacctt gcagttcggc
     2101 cagttcacga gcgcgatcga gggcgtcggc gatcgcgttg cgggcgctcg gtggcaacgg
     2161 ggccagcgca tcggcgccgt ccccccacag caactcgacg ccgtagcgct cgatcagatc
     2221 ggccaactcg tcgccgaccg tggcgaatcg gcgatcggga cggtgctcgg cccagtcgaa
     2281 cagcgagtag ccggcaccga ccaccatgcc ggcccgccgg gccgcggtga cgatctcacc
     2341 gacgacgtcg cgtcgtggtc cggcgtgcac ggacgaacgg gtggtgccgg gcgcatccca
     2401 ccagcagaag ccgtcgtgtt gcttgacgtc ctggaccagc aggttcatgc ccgcatcgag
     2461 ggccaactcg acatgttcgt cggcgtcgaa gcggtggtag ctgaggaacg gcacgaagtc
     2521 gtcgtagcgg gccacgtgcg accagcgatc gcggtggtag gcgagcactt cggccatcgg
     2581 tgatgggtgc ggcgcgatgc cggccagcgc gtcggtgccg aggtgggccc agtagtactc
     2641 ggccgcttcg ccgatcgggc tgaagctggg cacggtcgcg acgctggcgt gcagacgcat
     2701 gccgtagcgc cgctcgaccc accagtccgg aaccgtcacc ccgccatcct gccatgcgac
     2761 acctgtcgtc acccgtcttg cccgggcctg tcgacgtcac ctcgctggcg tgctggctgc
     2821 acgccagcgt acgttgtggt ctcgcagtcc tggcctgcca acatggaccc cgtgactgag
     2881 atcagcacgt ccgtgctcga cacgtcgtcc ggcgaccgac cagacggctg gctcgacgca
     2941 ccgctcgctc ggctcgacgc cgagctcacc cgtctcggac gggtggtggt ggccttcagc
     3001 ggtggcgccg actcggcctt tctggcccat cgggccac
//
LOCUS       DAORNF010000399         3025 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_24542_length_3025_cov_1.401684, whole genome shotgun sequence.
ACCESSION   DAORNF010000399 DAORNF010000000
VERSION     DAORNF010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3025
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24542_length_3025_cov_1.401684"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            253..741
                     /locus_tag="PLV68_14620"
     CDS             253..741
                     /locus_tag="PLV68_14620"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HRE02531.1"
                     /translation="MTTVSTTHTRPFRFGYQVSRGLDDLREQARAAEAGGFDVISTWD
                     HVGTTISPLLALVAMADATERIRFCPMVINNDFHHPAQLAMEFADLDQLSGGRAELGI
                     GAGHSFTEYAAMGLAFGTHDEIAAHLLSCRERWGISYFTVRTIDDMAPIIERLRAADA
                     GR"
     gene            755..>1105
                     /locus_tag="PLV68_14625"
     CDS             755..>1105
                     /locus_tag="PLV68_14625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02532.1"
                     /translation="MGRRANIEASVASLRAEAAAFDGTALDWQAGAEGLCGEARAFME
                     SMWWTSTFGPRRCGVEPTQRASPRSSECFSLGGGPIEMRFSDTAKRYEVFRQLAVAAT
                     LITRRGTSALGPEWR"
     assembly_gap    1106..1184
                     /estimated_length=79
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1291..>1649
                     /locus_tag="PLV68_14630"
     CDS             1291..>1649
                     /locus_tag="PLV68_14630"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRE02533.1"
                     /translation="MRTSTRLIVSIAAACGLLVSACGSDDKAAETTTTTAAPTSTSAA
                     PTTTAAATTTSAAATTTEAPEPAVEGDITVFAAASLTAAYTEIGEAFMTEYPDAEVVF
                     NFAASSELVAQITEGAPA"
     assembly_gap    1650..1675
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1676..>1940
                     /locus_tag="PLV68_14635"
     CDS             <1676..>1940
                     /locus_tag="PLV68_14635"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRE02534.1"
                     /translation="KKLTDAAGNSGDPRVFATSSLQIIVEPGNPKGITSLEDLAKSDV
                     LYVTCAPEVPIGKYSAQVLDAAGVTVTPVSLEENVKGIVTKVTL"
     assembly_gap    1941..2070
                     /estimated_length=130
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2328..2450
                     /estimated_length=123
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2451..3007
                     /gene="modB"
                     /locus_tag="PLV68_14640"
     CDS             <2451..3007
                     /gene="modB"
                     /locus_tag="PLV68_14640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012226303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HRE02535.1"
                     /translation="VRALCTLSMVLPPVVGGVALFFSFGRRGLFGQYLYRWLGFRLPF
                     TTWGVIVAQTFVAMPFLVLTVEAALRQLDGRYEGAARTLGASRWYVFRRVTLPTIRPA
                     LIAGAVLAWARALGEFGATITFAGNFPGRTQTMPLATYLALETNQEEALILSLLLIAV
                     SFAVLAGLRDRWLGGPGAQGAGLR"
BASE COUNT          412 a          935 c          881 g          439 t
ORIGIN      
        1 cgagttggtt gctggcgcgt ttgtggaacc cgcggcgtcg tcgctgccgc aggccgcgac
       61 ggtcagcgcc gtgagaagag cgacggcgat cgaacggcct cgcgcccggt gatggcggtg
      121 agtccgacac tgatcggggc agttattggt agaacgaaaa cgaagtaact ggggtttgtt
      181 catcatgcct aacactatgg atcaggcggt gccgcggcgc aaccccgcgt gacgagccgc
      241 cagactgcgt ctgtgacgac tgtcagcacg acgcacaccc gacccttccg cttcggctat
      301 caggtcagcc ggggcctcga cgatctgcgc gagcaggcca gagccgccga ggccggcggc
      361 ttcgacgtga tcagcacctg ggaccacgtc ggcacgacca tctcgccgct gctggcgctg
      421 gtggcgatgg ccgacgcaac cgagcgcatt cggttctgcc cgatggtgat caacaacgac
      481 ttccaccacc cggcgcaact ggcgatggag ttcgccgacc tcgaccagct ctcaggcggc
      541 cgggccgagc ttggcatcgg cgcggggcac tcgttcaccg agtacgccgc aatgggactg
      601 gcgttcggga cccacgacga gatcgctgcc cacctcctgt cgtgccgcga gcgatggggt
      661 atctcctact tcacggtgcg tacgatcgac gacatggccc cgatcatcga gcgcctccgc
      721 gccgccgacg ccggtcgctg acaggaccag gctgatggga cgacgggcga acatcgaggc
      781 gagtgtcgcc agcctgcgcg ccgaggcggc ggctttcgac ggcaccgcgc tcgactggca
      841 agccggcgca gagggactgt gcggtgaagc tcgcgcgttc atggaatcga tgtggtggac
      901 gtcgaccttc gggccgcgac gctgcggcgt cgagcccacc caacgcgcgt cgccccgttc
      961 cagcgagtgc ttctcgctcg gcggcggacc gatcgagatg cgcttcagcg acacggccaa
     1021 gcggtacgag gtgttccgtc aactcgccgt cgcggccacg ctcatcactc gccgcggcac
     1081 gtcggcgctc ggccccgagt ggcggnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntcgaac gttgactcgt
     1201 cgccgccaga cctggccgcg cagcgtgacg ttcgcgtgaa ctggatcacc agtccactcg
     1261 attagtcacc ctgtgactaa tctcagcgcc atgcgaacat ccactcgtct catcgtgtcc
     1321 atcgccgcgg cgtgcggcct cttggtgtcg gcctgcgggt ccgacgacaa ggccgccgaa
     1381 accaccacga ccacagcagc gccgacgtcg acctccgcgg caccgaccac gaccgccgca
     1441 gccacgacca cctcggccgc ggcaaccacc accgaggcgc ccgagcccgc tgtcgaaggt
     1501 gacatcaccg tgttcgcggc cgcgtcgctg accgctgcct acaccgagat cggcgaggcg
     1561 ttcatgaccg agtacccgga cgccgaggtg gtgttcaact tcgcggcctc gtccgagctc
     1621 gtcgcgcaga tcaccgaggg agcacccgcn nnnnnnnnnn nnnnnnnnnn nnnnngaaga
     1681 agctgaccga tgccgccggc aacagcggcg atccgcgggt gttcgccacc agctcgctgc
     1741 agatcatcgt cgagcccggc aacccgaagg gcatcacgtc gttggaggac ctggccaagt
     1801 ccgacgtgct ctacgtgacc tgcgcccccg aggtgccgat cggcaagtac tcggcccagg
     1861 tgctcgacgc agctggcgtc accgtcacgc ccgtctcgct ggaagagaac gtgaaaggta
     1921 tcgtcaccaa ggtgaccctg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn agcccccaac cccaccgggg ccgccgcatt
     2101 cgtcgacttc gtgctgagcg agcaaggtca gaagatcctg gcctcgtacg ggttcaccgc
     2161 tccgtgaccg acgcggcgac gccgcgcgtt cggacgacgc gcaggggcag gagggaacga
     2221 cttcccgccc ctgcgctcgt actcgcgctg gtcgccatcc tgttcttcac cctgcccttc
     2281 gtcgggctga tctggcgggc accgtggagc cgagcgtggg atgttctnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cggtgcgcgc
     2461 cctgtgcacg ttgtcgatgg tgctgccgcc cgtggtcggc ggtgtcgccc tgttcttctc
     2521 cttcggccgt cgcggcctgt tcggccagta cctgtaccgc tggttagggt ttcgcctgcc
     2581 gttcaccacc tggggcgtga tcgtcgccca gacgttcgtc gccatgccgt tcctggtgct
     2641 cacggtcgaa gccgcgttgc gccagctcga cggacgctac gagggcgcgg cgcgcacgct
     2701 cggagcgagc cggtggtacg tgttccggcg ggtcacgctg cccaccatcc gtcccgcgtt
     2761 gatcgccggt gcggtgctgg cgtgggctcg ggcactcggc gagttcggtg ccaccatcac
     2821 gttcgcgggt aactttcccg gccgcaccca gacgatgccg ctcgccacct acctcgccct
     2881 ggagaccaac caggaggaag cgctgatcct cagcctgctg ctgatcgccg tgtccttcgc
     2941 ggtgctggcc ggcctgcgcg accgatggct gggcgggccg ggcgctcagg gtgcaggact
     3001 cagatgaagg tgccgcgatg accct
//
LOCUS       DAORNF010000400         3021 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_24593_length_3021_cov_1.476062, whole genome shotgun sequence.
ACCESSION   DAORNF010000400 DAORNF010000000
VERSION     DAORNF010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3021
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24593_length_3021_cov_1.476062"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>370)
                     /locus_tag="PLV68_14645"
     CDS             complement(<1..>370)
                     /locus_tag="PLV68_14645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02536.1"
                     /translation="ALAALVTVPLIVALVALRGRHWSPVLDLAMTEFRVADVGGAHTP
                     LIGLPGRIGQMPFNQGSHPGPLSFYLLAPSYRGFGSSAWSLEVATVVLHLAAVVSALA
                     LAWRRGGVRLTIAVGVLLALT"
     assembly_gap    371..525
                     /estimated_length=155
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<526..1024)
                     /locus_tag="PLV68_14650"
     CDS             complement(<526..1024)
                     /locus_tag="PLV68_14650"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRE02537.1"
                     /translation="MKRFAELFKAALVARTRVPDLQLVIVGEGPERVVLESLIAAHDA
                     AGWVSLRGHIGRHELLALYQQAWLVTSASLAEGWGLTLTEAAACGVPAVATDISGHRC
                     SVVDGETGVLADLDHLGDTIADVLQQPQRRAELAAAALRRARELTWDNSALGIIDALH
                     HQVLLR"
     gene            complement(942..1682)
                     /locus_tag="PLV68_14655"
     CDS             complement(942..1682)
                     /locus_tag="PLV68_14655"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRE02538.1"
                     /translation="MPVTSQLPVTPQFDDYAAEMIRLGARRVHVLAWRDLDDIDAGGS
                     ELHAHEFMRRFAAAGLNVTQRTSAAAGLSATDRRDGYDVVRRGSRYSVFPRAIASELT
                     GRMGRFDALIEVWNGVPWLTPLWCRKPRIMFLHHVHGPMWDQILPKPLAAAGRLMETR
                     LAPPLYRRTATLTPSDATRDELIALGFKPDRAQRCRPVLPSRRRAVAGAADRRRGSSR
                     AGEAVRRTVQGRAGGQDPRARPATGHRR"
     assembly_gap    1949..1996
                     /estimated_length=48
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1997..>3021)
                     /locus_tag="PLV68_14660"
     CDS             complement(<1997..>3021)
                     /locus_tag="PLV68_14660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02539.1"
                     /translation="HGWMWWRRRGQRIVVPSVIGLVAGLIPMFVHLAMAGIGDSIRGM
                     VLDPVFDLRPGRELPRPPEWSLVNGALQAISEGVPARWPLPAPSASQQLFLWFFAMLI
                     VAIGTPIVGWRLLRRASNRIDAGSSDRGVALMMGGLFGAGLIQQGLQRPDSTHLAWVV
                     CVCFSLAPVTAWELLGRYAPALRERLRWRQGTAIGLAVVMLLVICPFFTYRHYLLHSR
                     VSVGNLPPPHHIVRGDREFWVGDYWVGVAGREVVADLEAWSQPGERLLVGPADLARTI
                     YSDVMFYYFFPELTPATYYIEMDPGLADREGSSLAADVASADWVLLTNFWTGWNEPNA
                     SSDRGSD"
BASE COUNT          472 a          990 c          988 g          368 t
ORIGIN      
        1 gtcagggcga ggaggacgcc gaccgcaatg gtgaggcgta caccgccacg ccgccaggcg
       61 agcgcgagtg ccgacaccac cgcggccaga tggagcacca cggtggcgac ctccaacgac
      121 cacgccgacg agccgaaccc gcggtagctg ggcgcgagca ggtagaagct gagcgggccg
      181 gggtggctgc cctggttgaa gggcatctgg ccgatgcggc cgggcagacc gatcagcggg
      241 gtgtgcgctc cgcccacatc ggcgacgcgg aactcggtca tcgccaggtc gagcacgggg
      301 ctccagtgcc gtccgcgcaa cgcaaccagg gccacgatca gcggcaccgt caccaacgcg
      361 gcgagcgccg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncgcgc agcagcacct
      541 gatgatggag cgcgtcgatg atgccgagcg ccgagttgtc ccaggtcaac tcgcgagccc
      601 ggcgcagcgc tgccgcggcc agttcggccc gccgctgcgg ttgctgcaac acgtcggcga
      661 tcgtgtcacc caagtggtcg aggtcggcga gcacgccggt ctccccgtcg accacgctgc
      721 agcggtgtcc actgatgtcg gtggccaccg ccggcacacc gcacgcggcc gcttcggtca
      781 gcgtgagccc ccacccttcg gccaacgatg cactcgtcac cagccacgcc tgctggtaca
      841 gggccagcaa ctcgtggcga ccgatgtggc cgcgcaggct gacccacccg gcggcgtcgt
      901 gggcggcgat caacgattcc agcacgactc gctctggccc ttcaccgacg atgaccagtt
      961 gcaggtcggg cacgcgggtc ctggccacca gcgcggcctt gaacagttcg gcgaaccgct
     1021 tcaccggcgc gagacgaccc acgccgacga tcagcggcac cggcgacggc tcgccgccgg
     1081 gatggaagaa cgggtcgaca ccgttgggcg cgatcgggct tgaagcccag ggcgatcagt
     1141 tcgtcacggg tcgcgtccga aggggtcagc gtcgcggtgc ggcgatacag gggcggggcg
     1201 agacgggtct ccatcaaccg gccggcggcc gccaacggct tgggcaggat ctggtcccac
     1261 atcgggccgt ggacgtgatg caagaacatg atccgcggct tgcggcacca cagcggcgtc
     1321 aaccacggca cgccgttcca cacctcgatc aaggcgtcga agcgccccat ccgcccggtg
     1381 agctccgatg cgatcgcccg cgggaacacg ctgtagcggc taccccggcg taccacgtcg
     1441 taaccgtcgc gccggtcggt cgcggacaac ccggccgcgg ccgatgtgcg ctgggttacg
     1501 ttcaggcccg ccgcggcgaa gcggcgcatg aactcgtgcg cgtgcagctc gctgcccccg
     1561 gcgtcgatgt cgtccaggtc acgccacgcc aagacgtgca cccggcgggc accgaggcgg
     1621 atcatctctg cggcgtagtc gtcgaactgc ggcgtcacgg gaagctggct ggtcaccggc
     1681 atgaaggccc aagcgtaggg ccgagaactc gtcggcccgc catccttcgc gctggctggt
     1741 cgctcgcgct ggcgggccct tgctggttcg ttctattcgc cgtcgaggcg cgggtcgata
     1801 tagcccgcgg cgcgctggcg ggccgagatc acgcccgcgt cagcggggct gatcccgtcg
     1861 ccgtgttgac cgggcgggca gcgccggtag agcagtacca acgaggccgt gctcggatag
     1921 tcgggatgcg gttcgcgggg gtactcccnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnncgtc cgagccgcgg tcggacgatg cgttgggctc gttccatccc
     2041 gtccagaagt tggtcagcag cacccagtcg gccgaggcga cgtcggctgc caggctcgat
     2101 ccctcccggt cggcgagacc ggggtccatt tcgatgtagt acgtcgccgg ggtcagctcc
     2161 ggaaagaagt agtagaacat cacgtcgctg tagatggtgc gggccagatc cgctgggccg
     2221 accaacaacc gctcgccggg ctgcgaccac gcttcgagat cggcgaccac ctcgcggcca
     2281 gcgacgccga cccagtagtc gccgacccag aactcgcggt cgccacggac gatgtggtgc
     2341 ggcggcggga ggttgccgac gctgacccgg ctgtgcagca ggtagtggcg gtaggtgaag
     2401 aacggacaga tgaccaacag catcaccacc gccagtccga tcgcggtgcc ctgccgccag
     2461 cgcagccgct cccgcagagc cggcgcgtag cggccgagca gttcccacgc ggtgaccggt
     2521 gcgagcgaga agcacacgca cacgacccac gccaggtgcg tcgagtcggg tcgctgcaga
     2581 ccctgctgga tcaacccggc gccgaacagc ccgcccatca tcagcgccac accgcgatcg
     2641 ctgctgccgg cgtcgatccg gttgctggcg cgacgcagca ggcgccaacc gacgatcggg
     2701 gtgccgatcg cgacgatcag catcgcgaag aaccacagaa agagctgttg cgatgccgac
     2761 ggcgcgggca gcggccaacg tgcgggcacc ccttcggaga tcgcttggag cgcgccattg
     2821 accagcgacc actcgggcgg ccgcggcagc tcacggccgg ggcgcaggtc gaacaccggg
     2881 tcgagcacca tgccgcggat gctgtcgccg atgcccgcca tcgcgaggtg gacgaacatc
     2941 gggatcaacc cggcgaccag accgatcacg ctgggcacga cgatgcgctg gccgcggcgg
     3001 cgccaccaca tccacccgtg g
//
LOCUS       DAORNF010000401         3019 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_24615_length_3019_cov_1.853576, whole genome shotgun sequence.
ACCESSION   DAORNF010000401 DAORNF010000000
VERSION     DAORNF010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3019
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24615_length_3019_cov_1.853576"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>345
                     /locus_tag="PLV68_14665"
     CDS             <1..>345
                     /locus_tag="PLV68_14665"
                     /inference="COORDINATES: protein motif:HMM:NF019886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRE02540.1"
                     /translation="GQLSEASTGLVTTAIRNRFIDGARAAAGEERRLRLVAVTDAEPV
                     VDGPDVSDGAAGSAERDGHDAGGGEGDGNDVAAAMARLSDRDRAALVLRYVDELSVAQ
                     VAAELNLSVHAT"
     assembly_gap    346..447
                     /estimated_length=102
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <448..801
                     /locus_tag="PLV68_14670"
     CDS             <448..801
                     /locus_tag="PLV68_14670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02541.1"
                     /translation="PDGELRSGFEAQLRARLAREWHRSPRRSADDRRRRRSMWLKVAC
                     AVVVVGVGAAVVIARTSGDDTVQSPADTAPQPPDTTPDSLPGTVPTTDPTPPSDPTPP
                     TDPASPDARPVTVGC"
     gene            821..2881
                     /locus_tag="PLV68_14675"
     CDS             821..2881
                     /locus_tag="PLV68_14675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02542.1"
                     /translation="MLAPRALRSLVSAPWNAEGVDRVLVGPDGTIAVVNHAGGSDVRD
                     VLELQRSGSTLGTGLTLTGMVDVAFGPDGLLYVLTSDGSGGSLVTAYDVATEYAWTAT
                     AETATEQPCTALDVRGLAVFCDTADVTAEPHRPIAGATIANAGDGRFTVESINGNVDA
                     RWDVSAAGMTVGRAWRTPVQGADRTVRSAAVVELIDAEGITWLTVLDTAEEAVAVPLQ
                     PVGLQPLGLSGTDLIALDEAGATVVSVDLASLIAGRELAVNRFVPECSEWATAADSSP
                     APDTAELDAAVSGSFGPLSLTPNLVITLPSYVTEWYPAGLPAHGDAVAVPGGWLVTIR
                     PTSDGLSQLSQSMLASITHDGSVRWMRCLDAPLDVLPVDGETALLISNVFSSESAVLD
                     VISLADGSVLRDFHTDLVAAGVSAAGAERLSIWSGSDSRLLLAPRWDVGQFDPSVDSL
                     VLVDLTDLGIELVPWPLNTTDLINSFGFADDGTTLTVDGTLAYVDGDWTSDPTVAPPR
                     VTFYGADTPDIQELTRVAADGSVIWRRDDLEDPSTEGFQSAVIGDIVLVRACRNNDDQ
                     PGCQFGEDGSNDFVLAALDVATGESRWELEGSHGVSIVADGYALVANEALGWHMIDLA
                     TGAMVDGQQWPSDATLGTGCCAADETEHTDRRGAAIVSVYVNTMSIYYPSAATRPTTT
                     LTLP"
BASE COUNT          434 a         1052 c          974 g          457 t
ORIGIN      
        1 ccgggcagtt gagtgaggcc agcaccggcc tcgtcaccac cgcgatccgc aaccggttca
       61 tcgacggcgc aagggctgcg gccggtgagg agcgtcggct gcgcttggtc gcggtcaccg
      121 acgccgaacc agtggtcgac gggcccgatg tgtccgacgg cgcggcgggc tccgccgaac
      181 gcgatggcca cgatgcgggc ggcggcgagg gtgacggcaa cgacgtcgcc gcggccatgg
      241 cccgcttgtc cgatcgcgac cgggccgcgc tcgtgttgcg ctacgtcgac gaactgtccg
      301 tcgcccaggt cgcagccgaa ctgaacctgt ccgttcacgc caccgnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnccc gacggcgagt tgcgctccgg cttcgaagcc
      481 cagctgcgag cacgtctcgc ccgcgaatgg caccgctcgc cacggcgcag cgccgacgac
      541 cggcgtcgcc gccgctcgat gtggctgaag gttgcctgcg ccgtcgtcgt ggtcggcgtc
      601 ggcgcggccg tggtgatcgc ccgtacgtcg ggtgacgaca cggtgcagtc gcctgccgac
      661 accgcaccgc agccgcccga cacgacaccc gactcgctgc ccggcaccgt gccgacgacc
      721 gacccgaccc caccgtctga cccgacaccg cccaccgatc cggcatcgcc agacgctcgc
      781 cccgtcaccg tggggtgttg atgggcaagg tgacgcaccc gtgctcgcgc cgcgcgccct
      841 gcggtcgttg gtcagcgccc cgtggaacgc cgagggtgtc gatcgggtgc tggtcggccc
      901 ggacggaacg atcgcggtgg tcaaccatgc aggcggttcc gatgtgcgtg acgtgctcga
      961 gttgcaacgc agtggttcga ccctgggcac cggcctgacg ctgaccggca tggtggacgt
     1021 agccttcggc cccgacggtc tgctgtacgt cctgaccagc gacggctccg gcggctcgct
     1081 cgtcactgcc tacgacgtcg ccaccgaata tgcatggaca gccaccgcgg agaccgccac
     1141 cgagcagcca tgcacggcac tcgacgtgcg tggattggcc gtgttctgcg acacggccga
     1201 cgtcacggcc gagccgcacc gaccgatcgc tggcgcaacg attgccaacg ctggcgacgg
     1261 tcggttcacg gtcgagtcga tcaacggcaa cgtcgatgcc cgttgggacg tctcggccgc
     1321 gggaatgaca gttggacggg catggcgcac gcccgtccaa ggtgccgacc gcacggtgcg
     1381 ctccgcggcc gtggtcgaac tgatcgacgc cgagggcatc acctggctca ccgtgctcga
     1441 cacggccgag gaggcggtgg cggtgccgct gcagccggtc ggcttgcagc cgctcggcct
     1501 ttcgggcacc gatctgatcg cactcgacga ggccggcgcc accgtcgtgt ccgtcgacct
     1561 cgcctcgttg atcgccgggc gcgagcttgc cgtcaaccgc ttcgtacccg agtgcagcga
     1621 atgggcgact gcagctgact cgtcccctgc gcccgacacg gccgagctcg atgccgccgt
     1681 ctccggttcg ttcggtccgc tgtcgctgac gcccaacctg gtgatcacat tgccgagcta
     1741 cgtcaccgag tggtacccgg ccgggcttcc cgcccacggt gacgcggtgg cagtgccggg
     1801 cggttggctg gtgaccattc ggccgacctc ggacggcctc agccaactca gccagtcgat
     1861 gctcgcctcg atcacccacg acgggtcggt gcgctggatg cgatgcctcg acgcgccact
     1921 cgatgtcctg cccgtcgacg gcgagacagc actgctcatc agcaacgtgt tctcgtccga
     1981 gtccgccgtt ctggacgtga tctcgctcgc cgacggctcg gtgctgcggg acttccacac
     2041 cgatctggtc gcggcggggg tctcggccgc gggtgccgag cggctgtcga tatggagcgg
     2101 ctccgactct cggttgctgc tcgctccccg ctgggacgtc ggacagttcg acccgagcgt
     2161 cgactcgctg gtgctggtcg acctgaccga cctcggcatt gaactggtgc catggccgtt
     2221 gaacaccacc gatttgatca actcgttcgg cttcgccgat gacggcacca cgctgacggt
     2281 cgacggcacg ctcgcctacg tcgacggcga ctggacgagc gacccgaccg tggcaccgcc
     2341 gagggtgacg ttctacggag cggacacacc cgacatccag gagttgacgc gagtagctgc
     2401 tgacggctcg gtgatttggc gccgcgacga tctggaagac ccgtcgaccg aagggttcca
     2461 gtcggcggtg atcggcgaca tcgtgttggt gcgggcctgc cgcaacaacg acgaccagcc
     2521 ggggtgccag ttcggcgaag acgggagcaa cgacttcgtg ctcgccgccc tcgacgtcgc
     2581 cacgggtgag tcgcgctggg agctcgaagg cagccatggg gtgtcgatcg tcgccgacgg
     2641 ttacgccttg gtggccaacg aagcgctcgg ctggcacatg atcgacctcg ccaccggtgc
     2701 gatggtcgat ggccagcagt ggccgtccga tgcgaccctc ggcaccggct gctgcgcggc
     2761 cgacgagacc gagcacaccg accgccgcgg cgcagcgatc gtctcggtct acgtcaacac
     2821 gatgtcgatc tactacccat cggccgccac ccgccccacg accaccctca cgcttccctg
     2881 acccacgccc cacgccgcgg tgacggtgtt cagttcaccc ccgcagtcgg ggccaactga
     2941 acaccgtcca gctaacccgc cccatgtgtg ctcgatccac ccctccagcc ggggtgaact
     3001 gaacaccgtc accgcggcg
//
LOCUS       DAORNF010000402         3011 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_24710_length_3011_cov_1.877199, whole genome shotgun sequence.
ACCESSION   DAORNF010000402 DAORNF010000000
VERSION     DAORNF010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3011
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24710_length_3011_cov_1.877199"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(198..818)
                     /locus_tag="PLV68_14680"
     CDS             complement(198..818)
                     /locus_tag="PLV68_14680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02543.1"
                     /translation="MPPSSALPSVVPSSRAAARLPLLSPLPATLVVTPWVDPVVEAVG
                     FDVRSPYVELFWLGVIGPTSTWLLRRLVTGFDHFPDGYELDLAETAAALGLSLTQGAQ
                     SPFGRALNRCVMFGAAYQSATSLAVRRLLPPLSGRHLGRLPEHLRHAHDHWVSSRPDA
                     GELDRAATLADAMASTGDEPHQIERQLLSVGISPRAAQEATRRLTR"
     assembly_gap    891..969
                     /estimated_length=79
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1017..>2016
                     /gene="moeB"
                     /locus_tag="PLV68_14685"
     CDS             1017..>2016
                     /gene="moeB"
                     /locus_tag="PLV68_14685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HRE02544.1"
                     /translation="MATFRDLLASAKAEITEIDTAPAAERIDAGGVVVLDVREPDEFD
                     QGALAGAVHIPRGHLEAQIENRIPDKTAPVIIYCAGGVRSAFAAKTMQELGYTDVVSL
                     IGGFGRWKDEGRAWTKPVVLTPDQRERYHRHLLLPEVGTEGQAKLLGSKVLLLGAGGL
                     GSPSALYLAAAGVGTIGIVDMDEVDASNLQRQVVHNVDRIGWRKVDSAKETLTKLNPD
                     VQVITYDTRLDASNILDIISGYDVIVDGADNFPSRYLLNDASVKLGIPVVHGSIFRFE
                     GMVSVFDPKHGPTYRDMVPEAPPAELAPSCAEAGVLGVLPGLVGTIQAEPSPSLPSL"
     assembly_gap    2017..2080
                     /estimated_length=64
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2276..>3011)
                     /locus_tag="PLV68_14690"
     CDS             complement(2276..>3011)
                     /locus_tag="PLV68_14690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M17 family metallopeptidase"
                     /protein_id="HRE02545.1"
                     /translation="SGHVILVGKGVTYDSGGISLKPSDPMHAIMKMDMSGAAAVLAAI
                     AALPALGCTTAVTAYMMCTDNMPSGSALRLGDVLTIRNGKTVEIHNTDAEGRLILADG
                     LSLAAEQKPDAIVDICTLTGAVLGALGDKMAGVLGNNQAWVDQVRAAGTRTDENTWQL
                     PLVREYRKALDSNVADMKNVGGPYAGTITASLFLDEFNAGVPWAHIDMAGTMKSDGDD
                     GWLSKGASGYGARLLIDLVTHFTPLA"
BASE COUNT          490 a          979 c          983 g          416 t
ORIGIN      
        1 catcgacacc atcgtcctcg accaaccccc caccgccctg gccatcatcg gcgccgcgct
       61 catcaccacc accctcaccg cgaccggacg tctgacgaag gtccggggat cgacgcgcta
      121 gacgatgccg cgatcgcgga aaccagcgtg agacgaaatc gtggtgttgg tgctggcacg
      181 ttcacaggca gccgcaacta gcgggtgagg cggcgggtgg cttcttgggc ggctcgcggg
      241 gaaatgccga cggagaggag ttggcgttcg atctggtggg gttcgtcgcc ggtgctggcc
      301 atcgcgtcgg ccaaggtggc ggcgcgatcg agctctccgg catcgggtcg gctcgacacc
      361 cagtgatcgt gcgcgtgacg gaggtgctcg ggcaggcggc cgaggtggcg gcccgacagc
      421 ggcggcagca gacggcgcac ggccagcgat gtcgccgact ggtacgcggc accgaacatg
      481 acacagcggt tgagagcccg cccgaacggg ctctgcgcac cctgcgtcaa cgacagaccg
      541 agcgccgcag cggtctcggc caagtcgagc tcatacccat cgggaaagtg gtcgaacccg
      601 gtgaccaggc ggcgcaacag ccaggtcgac gtcggcccga tcacccccag ccagaacagc
      661 tcgacgtacg ggctgcgcac gtcgaagccg acggcttcga ccacgggatc gacccacggg
      721 gtgaccacca gcgtcgcggg caacggcgag agcaacggga gacgagcagc ggcacggctc
      781 gacgggacga cggacggcag ggcagacgat ggtggcatgg gacgctccaa gggtcgcggc
      841 gatcggatgg gccgcatcga gcagcgatca acagggagac gtcacccctg nnnnnnnnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      961 nnnnnnnnng tcgggccgcc gtgccgtaat cccgactagt tcggtagact ttctccatgg
     1021 caacgttccg tgatctgctc gcctcggcca aggccgagat caccgagatc gacaccgcgc
     1081 ccgcggccga acgcatcgac gccggtggcg tggtggtgct cgacgtgcgc gagcccgacg
     1141 agttcgacca gggggcgctg gcgggagcgg tacacattcc ccgcggccac ctcgaagccc
     1201 agatcgagaa ccgcatcccc gacaagacgg ccccggtcat catctactgc gccggcggcg
     1261 tccgttcggc gttcgcggcc aagaccatgc aggagttggg ctacaccgac gtggtgtcgc
     1321 tgatcggcgg cttcgggcgg tggaaggacg agggtcgcgc gtggaccaag ccggtcgtac
     1381 tcacgcccga ccagcgcgag cgctaccacc gccacctgct gctgcccgag gtcggcaccg
     1441 agggtcaggc caagctgctc gggtcgaagg tgctgctgct cggcgccggt gggctcggct
     1501 cgccgtccgc gctctacctc gccgcagcgg gcgtcggcac gatcggcatc gtcgacatgg
     1561 acgaggtcga cgcgtcgaac ctgcagcgcc aggtggtgca caacgtcgac cgcatcggtt
     1621 ggcgcaaggt cgacagcgcc aaggagacgt tgaccaagct caaccccgac gtgcaggtga
     1681 tcacttacga cacccgcctc gacgccagca acatcctcga catcatcagc ggctacgacg
     1741 tgatcgtcga cggcgccgac aacttcccca gccgctacct gctcaacgac gcgtcggtga
     1801 agctgggcat cccggtggtg cacggcagta tcttccgctt cgaaggcatg gtcagcgtgt
     1861 tcgatcccaa gcacggcccc acctaccgcg acatggtgcc cgaagcaccc ccggccgagc
     1921 tggccccgag ttgcgccgag gccggcgtac tgggcgtgct gcccggcctg gtcggcacga
     1981 tccaggccga accttcgccg agcttgccat ccctcannnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn caagcccgac ccggccaact
     2101 gggtcaccta cgacaaccgc gacaagatcg aggtccacga cctcgacggc ttgtgcgcac
     2161 cctggaccgg ccactgaggg gaccggccac cgaggggacg gcaaccgagg ggcggcaacc
     2221 gagggacggc aactgatggt gtgcatcggg ctggcccgat gcacagcggc cgggatcagg
     2281 ccaacggcgt gaagtgcgtg acgaggtcga tcagcaggcg tgccccgtag ccgctggcac
     2341 ccttactgag ccagccgtcg tcaccgtcgg acttcatcgt tccggccatg tcgatgtggg
     2401 cccacggcac gccggcgttg aactcgtcga ggaacagcga ggcggtgatc gtgcccgcgt
     2461 acgggccacc gacgttcttc atgtcggcga cgttgctgtc gagcgccttg cggtactcgc
     2521 gcaccagcgg cagctgccac gtgttctcgt cggtgcgggt gcccgcggca cggacctgat
     2581 cgacccacgc ctggttgttg cccagcaccc cggccatctt gtcgcccagc gcaccgagca
     2641 cggcaccggt caaggtgcag atgtcgacga tcgcgtcggg cttctgctcg gcggccaggc
     2701 tgagcccgtc ggccaggatg agacggccct cggcgtcggt gttgtggatc tcgaccgtct
     2761 tgccgttgcg gatggtgagc acgtcaccca gccgcaaggc gctgccggac ggcatgttgt
     2821 cggtgcacat catgtaggcg gtcaccgcgg tggtgcagcc caacgcgggc agggcggcga
     2881 tggcggccaa gaccgcggca gccccgctca tgtccatctt catgatcgcg tgcatcggat
     2941 cgctgggctt gaggctgatg ccgcccgagt cgtacgtgac gcccttgccg accaggatca
     3001 cgtggccgct c
//
LOCUS       DAORNF010000403         3010 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_24722_length_3010_cov_1.575973, whole genome shotgun sequence.
ACCESSION   DAORNF010000403 DAORNF010000000
VERSION     DAORNF010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3010
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24722_length_3010_cov_1.575973"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..790)
                     /locus_tag="PLV68_14695"
     CDS             complement(<1..790)
                     /locus_tag="PLV68_14695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02546.1"
                     /translation="MVAALFIVLGASFIVYMLMTKAGDPLAWLVEIKDPIVREQTRKS
                     VVSGLRLDVHPIPRYFGWLWDLLRGDFGISARTRLPVSEDLGSRLLLTLKLVTASTIL
                     SVVLGIAVGIVTALRQYTGFDYVVTFFTFVFFSLPVFWVAVILKKYGGIDFNDWLRDG
                     AHFSPTVIVIFALCAGGISYTIAGGPTSRRAAIGAAGGVGVGLLVWYISASQWMLNPR
                     FGPIVLPQAQVDELMKDAEKGANATVTVDLEKQEITRPDGGVIKF"
     assembly_gap    872..887
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(929..>2400)
                     /locus_tag="PLV68_14700"
     CDS             complement(929..>2400)
                     /locus_tag="PLV68_14700"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRE02547.1"
                     /translation="DPIVLDADGNEVPFGEVEFVGDNEDELPEGWSYKLSDAINFDSA
                     SESMALITNFPTISDDGRSATFVWDSYYVDYQTAGPGVGVPAHVVGRLALGIDDPAEA
                     KQALLDAFEGKDGASLKKIADTWNTAFDSNELPDDPDLYLSAGPYLLTEYTKVSEMTF
                     EANPDYAWGPKPKVHTIVYKIIGDPTAAVQGLENEEIDIIQPQATADILSALTGLADR
                     GIEVITGDTGTYEHVDLVFNNGGPFDPAAYGGDAEIAKKVRQAFLKAIPRQDIVDRLI
                     KPLNPNAALRDSFTTVAGAPSYDTIAADNGSADYAAIDIEGAKALIAEAGVATPIDVR
                     FLFAQNNPRRGNEYELIRDSVAQVGFNLIDGRSPTWGPDLNNTGLYDASLFGWQSTSV
                     SVADSEANFRTGGQNNYGGYSSEVVDGLYDQLKGSTDPDEQQQLLLDIEKELWADAFG
                     VTLFQHPGVTAYNSTYVDNVSTIAIAPTVFWNAVGWTAA"
     assembly_gap    2401..2410
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2411..2833)
                     /locus_tag="PLV68_14705"
     CDS             complement(<2411..2833)
                     /locus_tag="PLV68_14705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02548.1"
                     /translation="MEPGGVGELNGSGCGTPHGPYEDDGSEPEGEVRVAWNDPLLSFN
                     SSTSRGNATANNNPLYLMSLGGFSYYDGDLNFVNNDQFGTCIIDSFDPLTITYTVNEG
                     VSWSDGTQIDAADMVLSWAASSTHFNSGDSVIAPNGETT"
BASE COUNT          510 a          902 c         1024 g          548 t
ORIGIN      
        1 cgaacttgat gacgccgccg tcgggccgcg tgatctcctg cttctcgaga tcgacggtga
       61 cggtggcgtt ggcgcccttc tcggcgtcct tcatcagctc gtccacctgc gcctgcggca
      121 gcacgatcgg cccgaagcgc gggttcagca tccactggct ggccgagatg taccacacca
      181 gcaggccaac accgactccg cccgcagctc cgatggctgc gcgtctagag gtgggtccgc
      241 cggcgatggt gtagctgatc ccgccggcgc acagggcgaa gatgacgatg acggtcggtg
      301 agaagtgggc tccatcgcgg agccagtcgt tgaagtcgat gcccccgtac ttcttgagga
      361 tgacggccac ccagaagacg ggcagcgaga agaacacgaa ggtgaagaac gtgaccacgt
      421 agtcgaagcc ggtgtactgc cgcagggcgg tgacgatgcc gacggcgatg ccgagcacca
      481 ccgacaggat cgtggatgcc gtgaccagct tgagggtcaa cagcagacgg ctgccgaggt
      541 cttcgctgac gggcaggcgt gtccgggcgg agatgccgaa gtcgccacgc aacaggtccc
      601 acagccaacc gaagtaacgc gggatgggat gcacgtcgag ccgcaacccg gagacgaccg
      661 actttcgggt ctgctcgcgg acgatggggt ctttgatctc gaccagccag gccagcgggt
      721 cgccagcctt ggtcatcagc atgtacacga tgaacgatgc gccgagcacg atgaacagtg
      781 cggcgaccaa gcgtctgacg atgaacgttg tcacgggcta cctggagggg ctcggcgggt
      841 cggagaaggg gagagaaacg gcacagggtg cnnnnnnnnn nnnnnnnccc ccctgccacc
      901 agtcaagtca gatcagtacg actgaccgtc aggctgcggt ccagcccaca gcattccaga
      961 acacggtcgg cgcgatcgca atcgtgctga cgttgtccac gtaggtgctg ttgtaggcgg
     1021 tgacgcccgg gtgctggaag agggtgacgc cgaaggcgtc agcccacagc tccttttcga
     1081 tgtcgagcag caactgctgc tgctcgtccg ggtcggtgct gcccttgagc tggtcgtaca
     1141 agccgtcgac gacctcgctc gagtagccgc cgtagttgtt ctggccaccg gtgcggaagt
     1201 tggcttccga gtcggcaaca gacaccgagg tcgactgcca accgaacagc gacgcgtcgt
     1261 agaggccggt gttgttgagg tccgggcccc aggtggggct acggccgtcg atgaggttga
     1321 agccgacctg agcgaccgag tcgcggatca gttcgtactc gttgccacgg cgcgggttgt
     1381 tctgggcgaa caggaaccgg acgtcgatcg gcgtggcgac gccagcctcg gcgatgagcg
     1441 ccttggcgcc ctcgatgtcg atcgcggcgt agtcggccga accgttgtcg gccgcgatcg
     1501 tgtcgtacga cggagccccg gccaccgtgg tgaacgagtc acgcagtgca gcgttggggt
     1561 tcagtggctt gatcagacga tcgacgatgt cctgacgggg gatcgccttg aggaaggcct
     1621 gacgcacctt cttggcgatc tcggcgtcac cgccgtatgc ggccgggtcg aacggaccac
     1681 cgttgttgaa caccaggtcg acgtgctcgt aggtcccggt gtcaccggtg atgacctcga
     1741 tgccacgatc ggccaggccg gtcaaggctg acaagatgtc ggcggtggcc tgcggctgga
     1801 tgatgtcgat ctcttcgttc tccaagccct gcacggcagc ggtcgggtcg ccgatgatct
     1861 tgtagacgat cgtgtgcacc ttcggcttcg gaccccaggc gtagtcgggg ttggcttcga
     1921 aggtcatctc cgacaccttg gtgtactcgg tcaacaggta cggaccagcg ctgaggtaca
     1981 gatcggggtc gtcgggcagc tcgttgctgt cgaacgccgt gttccacgtg tcggcgatct
     2041 tcttcagcga cgcaccgtct ttgccctcga acgcgtcgag cagggcctgc ttggcctcgg
     2101 cgggatcgtc gatgccgagc gcgagacgac cgaccacgtg cgcgggcacg cccacgccgg
     2161 gaccagcggt ctggtagtcg acgtagtagc tgtcccacac gaaagtggcc gagcggccgt
     2221 catcgctgat ggtcgggaag ttggtgatca acgccatcga ctcgctggcg gagtcgaagt
     2281 tgatcgcgtc cgacagcttg tagctccacc cctcgggcag ctcatcttcg ttgtcgccga
     2341 cgaactcgac ctcaccgaag ggcacttcgt tgccgtcggc gtcgagcacg atcggatcgc
     2401 nnnnnnnnnn ggtcgtctca ccgttggggg cgatgacgct gtcgccactg ttgaagtggg
     2461 tgctgctggc agcccaggac aacaccatgt cggcggcgtc gatctgggtg ccgtcgctcc
     2521 agctgacgcc ctcgttgacg gtgtaggtga tcgtcagcgg atcgaacgag tcgatgatgc
     2581 aggtaccgaa ctggtcgttg ttgacgaagt tcaagtcgcc gtcgtagtac gagaagccac
     2641 cgaggctcat caggtacagc gggttgttgt tggcagtggc gttgccacgg ctggtggacg
     2701 agttgaacga caacagcggg tcgttccacg ccacgcgcac ttcgccctcg ggctcgctgc
     2761 cgtcgtcttc gtacgggccg tgcggggtac cgcagccgct gccgttgagc tcgccgacgc
     2821 cacccggctc cactggagcg gtggtggtgg tgctgtcgcc gctgtccggc gtgctggccg
     2881 ccgtcgtggt cgaatcagca gccgttgtcg agctggcgtc cgaactggac ggagcagcgg
     2941 tcgactgtgt cgtgctggtg tctgccttgt catcgctgga gcaggccgca accatcaagg
     3001 ttgcgcccat
//
LOCUS       DAORNF010000404         3009 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_24738_length_3009_cov_1.550102, whole genome shotgun sequence.
ACCESSION   DAORNF010000404 DAORNF010000000
VERSION     DAORNF010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 3009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3009
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24738_length_3009_cov_1.550102"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>310)
                     /locus_tag="PLV68_14710"
     CDS             complement(<1..>310)
                     /locus_tag="PLV68_14710"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440616.1"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HRE02549.1"
                     /translation="PGSQGGPLEHVIAGKAVAFREAMHPSFRDYAHQIVANANALAAA
                     LAGQGFRLVSGGTDNHLMVVDLTPFDGELTGKIAQSVLDEAGITLNKNTIPNDPRSPF
                     V"
     assembly_gap    311..482
                     /estimated_length=172
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<483..>734)
                     /locus_tag="PLV68_14715"
     CDS             complement(<483..>734)
                     /locus_tag="PLV68_14715"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440616.1"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HRE02550.1"
                     /translation="DTIMGLSLDHGGHLTHGSPVNASGIFYDFVSYKVDPSDERIDYD
                     AMRDLARERQPKMIVAGTTSYPRRLDPEVFRSIADEVGAL"
     assembly_gap    735..960
                     /estimated_length=226
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1137..1613)
                     /gene="rpiB"
                     /locus_tag="PLV68_14720"
     CDS             complement(1137..1613)
                     /gene="rpiB"
                     /locus_tag="PLV68_14720"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HRE02551.1"
                     /translation="MSRIAIGADHAGYDLKQHLIGVLQGGGHDVADLGTHSTESVDYP
                     PICAAVVRAVRDGDADLGIVIGGSGQGEQLAANKVHGVRAALCNDLYTARMARAHNDA
                     NVLSIGGRVVGVGLAEEILATFLTTPFDGGRHQRRVEQLMAIEAEEAVTAPAARGH"
     gene            1731..>3009
                     /locus_tag="PLV68_14725"
     CDS             1731..>3009
                     /locus_tag="PLV68_14725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HRE02552.1"
                     /translation="MSIDPHTPVLIGAGQLAHHATSVADGLSPVALMAEAIRRATADA
                     GLDATPDADLFAVVGLLSYKHGDPAWLVARELGIDAGVTAITPMGGNSPQSLVNHCAA
                     RIQRGEMEIAILAGGEASRTRARAKREGVTLDWPVAPDDQVPVVIGDDLQMSHPAEAA
                     IQLFMPVQGYPMFETAIRAAAGRSVDDQRDLAAGLWSRFSAVAATNPHAWLQVERTAE
                     EIRNPSPSNRMIGFPYPKLMNSNNDVDMGAAVIICSVAKAEALGVPRDRWVFPHSGTD
                     CHEHAFMSNRWSFAETPAVALGGRLALELADMTIDDVDIVDLYSCFPSAVQLGAASLG
                     LPLDRQLTRTGGLCFAGGPWNNYVMHAIATVMNDLRTGAGRTGLVWANGGFATKHAFG
                     VYSTEPPAHGFRHAYPQDEIDAMPRREVAEGTDA"
BASE COUNT          392 a          858 c          935 g          426 t
ORIGIN      
        1 tgacgaacgg actgcgcgga tcgttgggga tcgtgttctt gttgagcgtg atgcccgctt
       61 cgtcgagcac gctctgggcg atcttgccgg tgagttcgcc gtcgaagggg gtcagatcga
      121 ccaccatcaa gtggttgtcg gtaccgccgc tgaccaggcg gaacccttgc ccggcaagag
      181 ccgcggcgag ggcgttggcg ttggccacga tctgatgggc gtagtcgcgg aagctggggt
      241 gcatcgcctc gcggaacgcc accgccttgc cggcgatgac gtgttcgagt gggccgccct
      301 ggctgccggg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      481 nncagcgcgc cgacctcgtc ggcgatgctg cggaagacct cggggtcgag ccgccgcggg
      541 tagctggtgg tgccggcgac gatcatcttc ggctggcgct cgcgggctag gtcgcgcatc
      601 gcgtcgtaat cgatccgctc gtcgctgggg tcgaccttgt acgacacgaa gtcgtagaag
      661 atgccgctgg cattgaccgg cgaaccgtgg gtcaggtggc cgccgtggtc gaggctgaga
      721 cccatgatcg tgtcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      961 cgcaggcgag gtgaagttct cgctggcgat cagctggatg ccgcccgact ggcgcgcgac
     1021 ctcggcgtcg atgaggccga acacctcgtt gtcgggatcg atgtcggaag gccaaggcat
     1081 gggagctcgc ttcggtggtt gaggtggcag ggggacgacg gacggcgcgg cgcgactcag
     1141 tggccgcggg cggctggcgc ggtgacagct tcttcggctt cgatcgccat cagctgctcg
     1201 acgcggcgct ggtggcggcc gccgtcgaag ggtgtggtga ggaacgtcgc caggatctct
     1261 tcggccaggc cgacccccac gacgcggcca ccgatgctga gcacgttggc atcgttgtgg
     1321 gcccgcgcca tgcgcgcggt gtacaggtcg ttgcacagcg cggcgcgcac gccgtgcacc
     1381 ttgttggcgg cgagctgctc gccctggccg ctgccgccga tgacgatgcc caggtcggcg
     1441 tcgccatcgc gcaccgcacg gacgacagcg gcgcagatcg gcgggtagtc gacgctctcg
     1501 gtgctgtgcg tgccgagatc ggccacgtcg tgaccgccgc cctggagcac cccgatcagg
     1561 tgctgcttga ggtcgtaccc ggcgtggtcg gctccgatgg cgatgcgtga cacgcccgag
     1621 agtgtaggac gccacccgcg ctggctccga aagacttcag ctccggccga ctcgactgtg
     1681 gctggcttca accccggggg gcgcaggggc gcgtcggtac agtcggggcg atgtcgatcg
     1741 atccccatac ccccgtcctg atcggagcgg gccaactggc ccaccatgcc acgtcggtcg
     1801 ccgacgggct gagtcccgtt gcgctgatgg ccgaagcgat ccgccgggcc acggccgacg
     1861 caggcctcga tgccacccca gacgccgatc tgttcgcggt ggtggggttg ctgagctaca
     1921 agcacggcga cccggcatgg ctggtggccc gggagttggg catcgatgcc ggtgtcaccg
     1981 cgatcacgcc gatgggcggc aacagcccgc agagcctggt caaccactgc gccgctcgca
     2041 tccaacgtgg cgagatggag atcgcgatcc tggccggcgg cgaggcatcg cgcacccggg
     2101 cgcgggccaa gcgcgagggt gtcaccctcg actggccggt cgcgcccgac gatcaggtgc
     2161 cggtggtgat cggcgacgac ctgcagatga gccacccggc cgaggcggcg atacagctct
     2221 tcatgcccgt gcagggctat cccatgttcg agacggcgat ccgggccgcg gctggccgca
     2281 gcgtcgacga ccagcgcgat cttgccgccg ggctgtggtc gcggttcagc gcggtggccg
     2341 caaccaaccc tcacgcatgg ttgcaagtgg aacgaacggc ggaggagatt cgtaacccgt
     2401 cgccatcgaa tcggatgatc ggcttcccat atcccaagct gatgaactcc aacaacgacg
     2461 tcgacatggg tgcggcggtc atcatctgta gcgtcgccaa ggccgaggca ctcggcgtgc
     2521 cgcgcgaccg gtgggtgttc ccccactccg gcaccgactg ccacgagcac gcgttcatgt
     2581 ccaaccggtg gagcttcgcc gagacgccgg cagtggccct gggcgggcgg ttggcactgg
     2641 agctcgcgga catgaccatc gacgacgtcg acatcgtcga cctgtacagc tgcttcccgt
     2701 ccgcggtgca gttgggtgcg gcttcgctcg gcctgccgct cgatcgccag ctcacccgca
     2761 ccggtggcct gtgctttgcc ggtggaccct ggaacaacta cgtcatgcac gcgatcgcga
     2821 cggtgatgaa cgacctgcgc accggcgcgg gacgcaccgg gttggtgtgg gccaacggcg
     2881 gcttcgccac caaacacgcg tttggcgtct attccaccga acctcccgcg cacgggttcc
     2941 gccatgccta cccgcaagac gagatcgacg cgatgcctcg ccgcgaggtc gccgaaggca
     3001 cggatgccg
//
LOCUS       DAORNF010000405         2995 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_24867_length_2995_cov_2.042177, whole genome shotgun sequence.
ACCESSION   DAORNF010000405 DAORNF010000000
VERSION     DAORNF010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2995
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24867_length_2995_cov_2.042177"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(136..426)
                     /locus_tag="PLV68_14730"
     CDS             complement(136..426)
                     /locus_tag="PLV68_14730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003272088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HRE02553.1"
                     /translation="MATKAELVDAVASKAGVTKADAERTIGAFFDYVVATTKKDGKVA
                     WPGFGSFSLSKRAARTGRNPQTGEAVKIKASKSVKFTASSTLKAALNPAKKK"
     gene            665..2128
                     /locus_tag="PLV68_14735"
     CDS             665..2128
                     /locus_tag="PLV68_14735"
                     /inference="COORDINATES: protein motif:HMM:NF014605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HRE02554.1"
                     /translation="MTGLAHASHAATVQDDAPSRDPSDGSGDELIGAAIDAVCATQPC
                     SQRFRVVLDTSVLVADPACLAGFADADVVVPLTVVEELDGLKTRSDDVGRAARAALRA
                     VEELRIRHGGSLATPVPIGADGNATLQIEINGVQKHLLVEHGLDPSKADNRIIGAALG
                     QAGKGPTRIVSNDAALRIKAAHLGVDASEHHPVGRAVATRPVGWTSLATDARVIDRLY
                     TDSAVPCVECESDPAIAATDENSFAVLRAGSQSALVRRRNDEFQLLSQSAPEAWGLRA
                     RSKEQRFALELLLDPEVSVVALDGRAGTGKTLVAVAAGLEQVVERRSYERLAIYRPLV
                     PVGRADVGFLPGGLDEKLDPWMSAIHDSIVALTDARCSRDAGALIDELTTRGKLSLES
                     VTFLRGRSLHRQFVVVDEAQNLEPTTLKTILTRIGEGTKVVFTGDTSQIDAPYMGESN
                     NALAVLIHAFAGQTCFGHITLSSCERSEVASLAAELL"
     gene            2206..2853
                     /locus_tag="PLV68_14740"
     CDS             2206..2853
                     /locus_tag="PLV68_14740"
                     /inference="COORDINATES: protein motif:HMM:NF013122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRE02555.1"
                     /translation="MEPLAASPPPRAAAPMRFAVVDVETSGLSPTRHRLLQVAVVVSD
                     ASGQVLDTWVSLVRPRWRWFFRLGPRHIHGLRRTDLRRAEPAPVVLRELSRRIDGAVL
                     TAHNAEFDMAFLRAAAQRAGVTLDLGTSLCTLWLSRHLDPDRTQSHRLADLATRYGVV
                     NGKPHDALEDALATATVLPHLLQAHGVTDASALVPHLRHAGEARPHSTPRVTSHS"
BASE COUNT          439 a         1060 c          979 g          517 t
ORIGIN      
        1 gttgaagcgg ccttggccgg agccgcagcc ttgctcgccg cgggcttggc cgcagccttc
       61 ttggccggag cggccttggc ggcgggcgcc ttcttcgttg ctgccacgtc agttcctttc
      121 agttgtccct gtgggtcact tcttcttggc cggattgagc gcggccttca acgtcgagct
      181 cgcagtgaac ttgacgctct tcgatgcctt gatcttgacc gcttcgcccg tctgcgggtt
      241 gcgcccggtg cgggccgccc gcttgctgag gctgaacgag ccgaagcccg gccatgcgac
      301 cttgccatcc ttcttggtgg tggcgaccac gtagtcgaag aaggcgccga tggtgcgctc
      361 ggcatccgcc ttcgtcacac ccgccttgct cgccaccgcg tcgaccaact ctgccttcgt
      421 tgccatgttg tctgattcct cttgtttgct ggggttactg cattacgcgg tgcacatcga
      481 acagcgtgga cccacggttt tcaagcattc cgatcgttcc gtttgcaacg atcggtgatg
      541 gcggcgcacc gccccgacat ggagccgaca tcaactgttc atcgaacggt ccgccactgc
      601 cgcgaagcgg cctggtagac cactgatcgg tgttgcgttc gtgttcacac aacggggggt
      661 ggcaatgacc ggacttgccc acgcgagcca tgcggccacg gtgcaggacg atgcgccgag
      721 ccgtgacccg agcgacgggt cgggcgacga gttgatcggt gcagccatcg acgcggtgtg
      781 tgccacccag ccgtgttcac agcgcttccg ggtcgtgctc gacacgtcgg tgctcgtcgc
      841 tgatccggcg tgtctggccg ggtttgccga tgccgacgtc gtcgtaccgc tgaccgtggt
      901 cgaagagctc gacgggttga agacccgttc cgatgacgtc ggccgcgccg ctcgtgccgc
      961 attgcgcgcc gtcgaggagc tgcgcattcg ccatggcggc tcgctggcaa ctccggttcc
     1021 gatcggtgct gacggcaacg ccacgctgca gatcgagatc aacggggtgc agaagcatct
     1081 gctcgtcgag cacggtctcg acccgtccaa agccgacaac cgcatcatcg gcgcggcact
     1141 cggccaagcg ggcaagggtc ccacgcggat cgtgtcgaac gacgctgcgc tgcgcatcaa
     1201 ggccgcgcac ctgggcgtcg atgccagcga gcaccatccg gtgggccgtg ccgtcgcgac
     1261 gcgtcctgtg ggatggacca gccttgccac cgacgctcgc gtgatcgacc ggctgtacac
     1321 cgacagcgcg gtgccgtgcg ttgagtgcga gtccgatccg gccatcgcgg ccaccgacga
     1381 gaacagcttc gcggtgctcc gggccggctc gcagtcggct ctcgtgcggc gccgcaacga
     1441 cgagttccaa ctgctgtcgc agagcgctcc agaggcgtgg ggactgcgcg ctcgttcgaa
     1501 ggagcaacgc ttcgcgctgg agcttctgct cgatcccgag gtgagcgtcg tcgccctcga
     1561 cgggcgggcg ggcaccggca agacgttggt cgccgtggcc gccgggctgg aacaggtggt
     1621 cgagcggcgc agctacgagc ggctcgcgat ctaccgcccc ctggtcccgg tcggacgagc
     1681 cgacgtcggc tttctgcccg gtggcctcga cgagaagctc gacccgtgga tgagcgcgat
     1741 ccacgactcg atcgtcgccc tcaccgacgc acgctgcagc cgcgacgccg gggcactgat
     1801 cgacgagctc accacccgcg ggaagctgtc gttggagtcc gtgaccttcc tccgaggccg
     1861 ttcgctgcac cgccagttcg tcgtcgtcga cgaagcccag aacctggaac cgaccacgct
     1921 gaagaccatc ctgacccgca tcggtgaagg caccaaggtg gtcttcacgg gtgacaccag
     1981 ccagatcgac gcgccgtaca tgggcgagtc gaacaacgcc ctcgcggtgc tgatccacgc
     2041 ctttgccgga cagacctgct tcggccacat cacgttgtca tcgtgtgaac gcagcgaggt
     2101 ggccagcctg gccgccgaac tgctgtgaac ccagcctcgg tgggccaagc gacgggcagg
     2161 tcatcactcc cagcgttgtg acacccgttg cgtagggtgg cagccatgga acccctcgcc
     2221 gcctcgccgc caccccgtgc cgccgcgccg atgcggttcg cggtggtcga cgtcgagaca
     2281 tcagggttgt cgcccacacg ccaccgcctg ctgcaggtcg ccgtggtggt cagcgacgcg
     2341 tcggggcagg tgctcgacac gtgggtcagc ctggtgcgtc cgcggtggcg gtggttcttc
     2401 cgcctcgggc cccgccacat ccacggcctg cgtcgcaccg atctgcgccg ggccgaaccg
     2461 gcaccggtgg tgctgcgcga gttgtcccgg cgcatcgacg gcgcggtgct cacggcccac
     2521 aacgccgagt tcgacatggc ctttctgcgt gccgcggccc agcgggcagg tgtcacgctc
     2581 gacctcggca cgtcgctgtg caccctgtgg ctgtcgcgcc acctcgatcc cgaccgcacc
     2641 cagtcgcatc gcctcgccga cctggccacc cgctacggag tcgtcaacgg caagcctcac
     2701 gacgcgctcg aagacgccct cgccaccgca acggtgctcc cccatctgct gcaggcccac
     2761 ggtgtgacag acgcatcggc gctcgtgccc cacctgcgcc atgcgggcga ggctcgaccg
     2821 cactcgacgc cgcgcgtcac gtctcactcg tagttggcaa gcaccgcggc tgcggtctcg
     2881 gcgccgagac cgacccactc cgcgggcgcg atcacggccg ccgcgggccc gagccgcaac
     2941 agcagatgcc cgacccaggt ctgcgatgtg gccggcatca tcaccgtcac ggtct
//
LOCUS       DAORNF010000406         2990 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_24921_length_2990_cov_1.641908, whole genome shotgun sequence.
ACCESSION   DAORNF010000406 DAORNF010000000
VERSION     DAORNF010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24921_length_2990_cov_1.641908"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..866
                     /locus_tag="PLV68_14745"
     CDS             <1..866
                     /locus_tag="PLV68_14745"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: protein motif:HMM:NF014685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HRE02556.1"
                     /translation="MTDGDGLGKARSGQRVGAGLVGALRFLTRVSIPGPGDVDHRDVV
                     PWFPVAGALIGAVVGTIAVGLGEILPAPLAATLAVVAGLLITGAFHEDGLADVADAFG
                     GGWNREQRLAILKDSRHGTYGVATLCSSIVVRVVAVATIASATNGQAVVFAGFVAAHT
                     LARAAAVGVLGFVRPAAPDVQGLGVAAGRELRRFTTIGSIITGLLLAGLATGWWVAPL
                     AGHRCRRSCGRGNPCSAQDRRCRRRRARCDRTGCRVSHPDRRRRTRRPPPPVVGMKAR
                     RDRGMQVWRHL"
     gene            903..1847
                     /locus_tag="PLV68_14750"
     CDS             903..1847
                     /locus_tag="PLV68_14750"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HRE02557.1"
                     /translation="MTGELPGAFDNRVARDLGVGIPIVQAPMGWIARAQLASAVSRAG
                     ACGIIETSSGELDNVKDEIRTMRDLTDRPFGVNIAQAFVRDPGIAEFVIDQGVRFVTT
                     SAGSPTKYTSILKAAGLTVYHVVPTLAAALKAVDAGVDGLVVEGGEGGGFKSPTPVST
                     MVLLPLVRSKVDVPIIAAGGICDGYTMAAAFALGAEGVQMGTRMVSSAESPVHHNWKQ
                     AIIDAAETDTLFLNDRHSPALRALRTDRTERLAHAEHNVFGDFGDPKALYFGGDMNAA
                     IALSGEVAGRIDSVRPVADIIADTVHEFTLATARLTTP"
     gene            2032..>2267
                     /locus_tag="PLV68_14755"
     CDS             2032..>2267
                     /locus_tag="PLV68_14755"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRE02558.1"
                     /translation="MPANPNTETVVTGTAHLGNAVRRAREQRGIDQATLADRSGTHRT
                     YVSKFETNPPRDTIGRLLRILDALNLELVIRPRQP"
     assembly_gap    2268..2418
                     /estimated_length=151
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2419..>2990
                     /locus_tag="PLV68_14760"
     CDS             <2419..>2990
                     /locus_tag="PLV68_14760"
                     /inference="COORDINATES: protein motif:HMM:NF019421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HRE02559.1"
                     /translation="GDRHTRTWLRGLLPEGQIRAMIAYDLGIAGTDDFALLAALGKDC
                     AGALSFRPAGDADDDEQISGPLTEAHIASLLRSLPTSPMGVGAGFRASLPGHQSKLLL
                     TRVDNDWYRPDGAPSTHILKPAVRELDDSTVDNEAYCQQLATAAGLPAAITTIEQFDG
                     VRVLTSQRFDRVRHGTHTVRIHQEDGCQAT"
BASE COUNT          493 a          988 c          894 g          464 t
ORIGIN      
        1 cgatgaccga tggcgatggg ttgggcaaag ctcgctccgg tcagcgtgtg ggggcggggc
       61 tcgtgggggc actgcggttt ctgacccgcg tgtccattcc aggtcccggc gatgtcgatc
      121 accgcgatgt cgtgccctgg ttcccggtgg ccggcgcact catcggagca gtcgtgggga
      181 ccatcgcggt cggcttgggc gagatacttc ctgctcccct cgcggccacc cttgccgtgg
      241 tcgcaggtct gttgatcacc ggcgcgttcc acgaggacgg gcttgccgac gtcgcggacg
      301 cattcggtgg gggctggaac cgcgaacagc gtttggcgat cttgaaggat tcgcgccatg
      361 gcacgtacgg ggtcgccacg ctctgtagct cgattgtcgt gcgcgtcgtc gcggtggcca
      421 caatcgccag cgcgacgaac gggcaggcgg tcgtattcgc cggattcgtc gccgcgcaca
      481 cgctggcacg tgccgctgca gtcggcgtgc tcggcttcgt ccgtcccgcc gcgccggacg
      541 tacagggcct gggcgtggcc gccgggcgcg agctgcgcag gttcaccacc atcggctcga
      601 tcatcaccgg gctcctgctg gccgggctcg ccaccggttg gtgggtcgcg ccactcgccg
      661 gccaccgctg tcggcggagc tgcggtcgtg gcaacccttg cagtgcgcaa gatcggcggt
      721 gtcgtcggcg acgtgctcgg tgcgaccgaa caggttgcag agtgtctcat cctgatcgcc
      781 gtcgccggac tcgccgccca ccaccacctg tggtgggcat gaaggcgcgg cgggatcgag
      841 gcatgcaggt atggaggcat ctctgatcgt tcgcgctggt gggcgctgcg taggctccgg
      901 cgatgactgg tgaacttcct ggagcgttcg acaatcgggt ggcccgcgat ctcggggtcg
      961 gcattccgat cgtgcaggca ccgatgggct ggatcgcccg ggcacaactc gcgtcggccg
     1021 tgtcgcgagc aggggcgtgc ggcatcatcg agacgtcgtc gggcgaactc gacaacgtga
     1081 aggacgagat ccggacgatg cgcgatctca ccgaccgccc attcggggtc aacatcgccc
     1141 aggccttcgt acgcgacccg ggcatcgccg agttcgtgat cgatcagggc gttcgcttcg
     1201 tcaccacgtc ggcgggatcg ccgacgaagt acaccagcat cctcaaagcc gccggactca
     1261 ccgtgtacca cgtcgtaccg acgcttgcgg ctgcgctgaa ggcggtcgat gcgggagtcg
     1321 acgggctcgt cgtcgaaggt ggcgaaggcg gcgggttcaa gagtccgacc ccggtctcga
     1381 ccatggtgct actgccgctg gtgcgatcga aggtcgacgt accgatcatc gccgcgggcg
     1441 gcatctgcga cggttacacg atggccgccg cattcgcgct cggtgccgaa ggcgtgcaga
     1501 tggggacgcg gatggtgtcg tccgccgagt cgccggttca tcacaactgg aagcaagcga
     1561 tcatcgacgc ggccgagacc gacaccctgt tcctcaacga ccgccactcc cctgcactgc
     1621 gtgcgctgcg caccgaccgc actgagcggc tggcccacgc cgagcacaac gtgttcggcg
     1681 acttcggtga ccccaaggcg ctgtacttcg gcggcgacat gaacgcggcg atcgcgctgt
     1741 ccggtgaggt cgcgggacga atcgattcgg tccgcccggt ggccgacatc atcgccgaca
     1801 ccgtccacga gttcaccctc gccaccgccc gcctgaccac cccctgacca cgtgtcaccg
     1861 accaagctga ctccgtcaaa cgtacgctgc tgaagactcc ccgcgatgcc gtcgcttgta
     1921 gctgatgcgg gagggtcgtc accaacaggt ggtgcgcgac cgcatttccg aacatcacgt
     1981 cggccaagtg gccgttcccg caaatcagca cactcgtact atgctggtct gatgcccgcg
     2041 aatccgaaca ccgaaactgt cgtgaccggg acggcacacc ttggtaacgc cgtacgtcgc
     2101 gcacgagaac aacgcggcat tgaccaggca accctcgccg atcggtccgg cacacatcgc
     2161 acctacgtgt cgaagttcga aaccaacccc ccacgcgaca ccattggacg gctgctgcga
     2221 atcctcgacg ccctcaacct tgagctcgtg atccgccccc ggcaaccnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnngg cgaccgccac acccgcacct ggctgcgcgg tctgctcccc
     2461 gaaggacaga tccgcgccat gatcgcctac gacctcggca tcgccggaac agacgacttc
     2521 gcgctcctgg cagcactcgg caaagactgc gccggggcgc tgtcgtttcg cccagccggc
     2581 gacgccgacg acgatgaaca gatcagcgga ccgctcaccg aagcacacat cgcgtcgctc
     2641 ctgcggtcgc tgccaacatc accaatgggt gtcggagccg ggttccgcgc atcactacca
     2701 ggccaccaaa gcaaactgct cctcacacgc gtcgacaacg actggtaccg ccccgacgga
     2761 gcaccgtcaa cacacatcct caaacccgcc gttcgcgaac tcgacgacag caccgtggac
     2821 aacgaggcgt actgccaaca actcgccacc gccgcgggcc tcccagcagc gatcaccacc
     2881 atcgaacaat ttgacggtgt ccgcgtcctc acctcccaac gattcgatcg agtacgtcac
     2941 ggaacccaca ccgtcagaat ccaccaagaa gacggttgcc aagccaccat
//
LOCUS       DAORNF010000407         2988 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_24937_length_2988_cov_1.816229, whole genome shotgun sequence.
ACCESSION   DAORNF010000407 DAORNF010000000
VERSION     DAORNF010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2988
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24937_length_2988_cov_1.816229"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="PLV68_14765"
     CDS             <1..257
                     /locus_tag="PLV68_14765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02560.1"
                     /translation="RLLAAFRAGGFHVIHTREGHRPDLSDLPANKRWRSRRIGAAENV
                     AVQLLDLGQIERGRVELEQLDEVGADEAGKRDTDAHALTP"
     gene            complement(239..1240)
                     /locus_tag="PLV68_14770"
     CDS             complement(239..1240)
                     /locus_tag="PLV68_14770"
                     /inference="COORDINATES: protein motif:HMM:NF025587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate and iron-dependent oxygenase
                     domain-containing protein"
                     /protein_id="HRE02561.1"
                     /translation="METVPVIDIAPFLEGTPEGIDSVARQIAHACEEIGFFQITGYGV
                     SEEFIQEVYDMSRRFFDLPESDKAVAAQPAPGQVRGWTSVGMESLSYSLDEAAPADLK
                     EKMDMGPFDVPDDEYHTSEEAGPHFAPNVWPTALPEMRPLWEQYFAAMSEVSRQVMRI
                     FAVGLGLDIDYFEDKIDEHISMFRAINYPDQVEPPLPGQLRAGAHSDYGSLTLLRQEQ
                     RPGSLQVMTKAGEWFDVPAIKGALVVNIGDLMSEWTNDRWVSTLHRVVNPPRDEANDS
                     RRISLVFFHQPNYDAMISCLPTCLKPGEEPKHAAISSGDHLFSKFVKQSTFGQGVSA"
     gene            complement(1330..2400)
                     /locus_tag="PLV68_14775"
     CDS             complement(1330..2400)
                     /locus_tag="PLV68_14775"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HRE02562.1"
                     /translation="MNEPLPNLGRRRFLQVSAGLVVGGGLLAACGSDEKKGESSTTSS
                     APSSSSTASTGGTAGPTEGLIGLTLNGLNDYTKGVTTGVYKALENTKYTLEVVQVNYD
                     AAQELAAAEAFLAKGIVGLVIQPNTAESAGAAAEKAVEKGVPAGNCIWPGPSDADKYF
                     VGVAEIDSVEGGRLIGEYLKANVTPGKVCVVQGVVGQGFSEKIDEGLDAALAGTDFEV
                     VVREQAFFDRTQAVGVVEKAFQAHPDLGIVVAYSASMSNGISQWLKDQGKDKVIHVSS
                     DADEEMITWMKTPYLSATRYYSSAQTGLIAANAVLASLRGETPQFRSVIEQVIATEDN
                     IDAVVEANPYMFFEYKDKVQNI"
     gene            complement(2393..>2988)
                     /locus_tag="PLV68_14780"
     CDS             complement(2393..>2988)
                     /locus_tag="PLV68_14780"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRE02563.1"
                     /translation="EWLGRKVVWGLQTPIWYFLIVVAIAAVVMRYSRFGRHVYATGSN
                     EEASYLAGIPTARTKVLCFVISSTLAGFAGLVAMARISTGDPRVGSQLALQAIAAVVL
                     GGGTLAGGRGSPLGTFVGVTLLAVVQSALTFNNVQTFWNDAVFGGVLIFAVVVTAVGD
                     LRRRRVGAKSILSSWFQRSPSPAPLTVSPQQGEKPHE"
BASE COUNT          525 a          963 c          975 g          525 t
ORIGIN      
        1 cgcgcctgct ggccgcgttt cgcgccggcg gcttccacgt gatccacacg cgcgagggcc
       61 accggcccga tctgtcggac ctgccggcca acaagcgctg gcgctcccga cgcatcggcg
      121 ccgcggaaaa tgtcgctgtg cagctcctcg acctcgggca gatcgaacgt ggtcgagtag
      181 aactcgaaca gcttgacgaa gtcggcgctg atgaagctgg caagcgagat actgacgctc
      241 acgcgctgac gccctgaccg aacgtcgact gcttgacgaa cttggagaac aggtgatcac
      301 ccgacgagat cgccgcgtgc ttcggttctt caccgggctt gaggcacgtg ggcaggcacg
      361 agatcatcgc gtcgtagttc ggctgatgga agaacaccag cgagatgcgc cgcgagtcgt
      421 tggcctcgtc gcggggcggg ttgacgaccc ggtgcaacgt ggagacccag cggtcgttgg
      481 tccattcgga catgaggtcg ccgatgttga ccaccagcgc acccttgatc gccgggacgt
      541 cgaaccactc gccggccttg gtcatcacct gcaagctgcc ggggcgctgt tcctggcgca
      601 gcagcgtcaa gctgccgtag tcggagtgcg caccggcgcg gagctggccg gggagcggcg
      661 gctccacctg atcggggtag ttgatcgccc ggaacatgct gatgtgctcg tcgatcttgt
      721 cctcgaaata gtcgatgtcg agcccgagac cgaccgcgaa gatgcgcatc acctgacgcg
      781 acacctcgct catggcggcg aagtactgct cccacaacgg gcgcatctcc ggcaacgcgg
      841 tgggccacac gttgggagcg aagtgcggac cggcctcttc ggacgtgtgg tactcgtcgt
      901 cgggcacgtc gaagggaccc atgtccatct tctctttgag gtcggcgggc gccgcctcgt
      961 cgagggagta ggacaacgac tccatgccga ccgaggtcca accgcggact tggcctggcg
     1021 ctggctgggc cgcgaccgcc ttgtcggact cgggcagatc gaagaagcga cgactcatgt
     1081 cgtacacctc ttggatgaac tcttcggata cgccgtaccc ggtgatctgg aagaagccga
     1141 tctcctcgca cgcatgcgcg atctgcctcg cgacagaatc gatgccttcg ggcgttcctt
     1201 ccaggaacgg tgcgatatcg ataactggaa cggtttccat gattcctctc ctgtcttttc
     1261 ccagatcacc gggacagcgt tgcaaccttg atagacgacg ggccgcagag cccgccatgg
     1321 ccagatggcc tagatgttct ggaccttgtc cttgtattcg aagaacatgt acgggttggc
     1381 ctcgaccacc gcgtcgatgt tgtcctcggt ggcgatgacc tgctcgatca ccgaacggaa
     1441 ctgcggggtc tcgccacgca gcgaggccag cacggcgttg gcggcgatga gaccggtctg
     1501 ggccgacgag tagtaacgcg tcgccgacag gtacggcgtc ttcatccagg tgatcatctc
     1561 ttcgtcggcg tcggacgaga cgtggatgac cttgtccttg ccctggtcct tcaaccactg
     1621 cgagatgccg ttggacatcg acgccgagta ggcgaccacg atgccgagat cggggtgtgc
     1681 ctggaacgcc ttttcgacca cgccgacggc ttgggtccgg tcgaagaacg cctgctcacg
     1741 cacgaccact tcgaagtcgg tgccggcgag cgcggcgtcg agaccctcgt cgatcttctc
     1801 ggagaagccc tggccgacga caccttgcac cacgcacacc ttgccgggcg tgacgttggc
     1861 cttgaggtac tcgccgatca gacggccgcc ttcgaccgag tcgatctcgg ccacaccgac
     1921 gaagtacttg tcggcgtcgc tcgggccggg ccagatgcag ttgcctgcgg gcacgccctt
     1981 ctcgaccgcc ttctcagccg cggcaccggc cgactcggcc gtgttcggct ggatgaccag
     2041 gccgacgatg cccttggcca ggaaggcctc ggcagcagcc agctcttgtg ctgcgtcgta
     2101 gttcacctgc acgacttcga gggtgtactt ggtgttctcc agcgccttgt agacaccggt
     2161 ggtcacgccc ttggtgtagt cgttgaggcc gttcagggtc aggccgatga gaccttcggt
     2221 cgggccggca gtgccgccgg tggaggcagt gctgctggac gatggcgcgg acgacgtggt
     2281 cgaggactcg cccttcttct cgtcgctgcc gcaggccgcg agcagcccgc cgcccacgac
     2341 gagcccggca gagacctgca ggaaacggcg tcggccgagg ttcggtagtg gctcattcat
     2401 gtggtttctc cccttgttgt ggactgaccg tcaacggtgc cggtgacgga gatctctgga
     2461 accaactcga caatatggac ttggccccga cccggcgacg gcgcaggtcg ccgacggctg
     2521 tgacgaccac ggcaaagatc aacaccccac cgaacaccgc gtcgttccag aacgtctgca
     2581 cgttgttgaa ggtcaatgcc gattgcacga cggcgagcaa ggtcacaccg acgaaggtgc
     2641 ccagcggcga ccccctgccg ccggccagcg tgccgccacc gagcacgacg gcggcgatcg
     2701 cctgcagtgc gagctgcgaa ccgacgcgag ggtcaccggt ggagatgcgc gccatggcga
     2761 ccaagccggc aaagccggcg agggtgctgg agatcacgaa gcacagcacc ttggtgcgtg
     2821 cggtcggaat gccggccagg tacgacgctt cctcgttgga gccggtcgcg tagacgtggc
     2881 gcccgaagcg ggagtagcgc atgaccacgg ccgcgatggc caccacgatc aggaagtacc
     2941 agatcggtgt ctgcaggccc cacaccacct tgcgcccgag ccactcga
//
LOCUS       DAORNF010000408         2982 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_25024_length_2982_cov_1.154424, whole genome shotgun sequence.
ACCESSION   DAORNF010000408 DAORNF010000000
VERSION     DAORNF010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2982
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25024_length_2982_cov_1.154424"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            47..>305
                     /locus_tag="PLV68_14785"
     CDS             47..>305
                     /locus_tag="PLV68_14785"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRE02564.1"
                     /translation="MTLPIARDLAAIGVRVNTVAPGLVDTPIYDTFPDPEGFKARLGR
                     GVLFPNRLGAAEDLASMIVECVTNSYMNAETIRVDGGIRMQP"
     assembly_gap    306..474
                     /estimated_length=169
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<475..>720)
                     /locus_tag="PLV68_14790"
     CDS             complement(<475..>720)
                     /locus_tag="PLV68_14790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02565.1"
                     /translation="VAHLAVSVVACEARSAFFDDVFADPEIDAVYQNYRAEHLTRLIE
                     LVESGHDSGTVRACRPALVAEVLDAAAERLRHVHQVVP"
     assembly_gap    721..840
                     /estimated_length=120
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1137..1146
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1147..2211)
                     /locus_tag="PLV68_14795"
     CDS             complement(<1147..2211)
                     /locus_tag="PLV68_14795"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRE02566.1"
                     /translation="MTDTLNVQDLKVIDTDTHISEPADLWTSRLPAKFQDIAPRVQHN
                     EATGHDHWRVGDYWLMGSGFYAVAGWKEFPPNGPHDLDHPEVDKGSYDPNVRLQRMDE
                     HGIYAQVLYPNLIGFETGQFMKLGLDAALACVQVYNDFQTEFASTDPKRFIPITMVPF
                     WDLEESEKEIRRCHEMGHRGILFANKYEQIGLPAFTDTHWDPIYKTCAELDTSVNYHV
                     GFSSSTDGSAQAMTAMLANFSARAAAVGTAIGLMSNGDSIARIITSGVCERHPTVNFV
                     SVESGFGFIPYLLDSLDWHWKGYGASRETKSDMLPSEYFARQCYGTFWFETSTLRLLD
                     TYPDNFMFETDYPHPTAMSPG"
     assembly_gap    2287..2339
                     /estimated_length=53
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2395..>2610
                     /locus_tag="PLV68_14800"
     CDS             2395..>2610
                     /locus_tag="PLV68_14800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02567.1"
                     /translation="MDEHPNVAVIRAALEHVGKGDIDAMFALWENEFVYYGFDAEGRN
                     RECRNRADFTEIFGIGQRLLETHENEVI"
     assembly_gap    2611..2670
                     /estimated_length=60
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          496 a          789 c          815 g          470 t
ORIGIN      
        1 gatcgggcag gcggcctact ccgcgtcgaa agggggcgtg atcggcatga cgcttccgat
       61 cgcgcgggat ctcgctgcca tcggtgtccg cgtcaacacg gtggcacccg gtctggtcga
      121 tacaccgatc tacgacacgt tccccgatcc cgaagggttc aaagcccgtc tgggccgcgg
      181 cgtgctcttc cccaaccggc tcggtgccgc cgaggatctc gcatcgatga tcgtcgaatg
      241 cgtgaccaac agctacatga acgccgagac gatccgggtc gacggcggca tccgcatgca
      301 accgannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncggcac
      481 gacctggtgc acgtgccgca aacgctcggc ggcagcatcg agcacctcgg cgaccagcgc
      541 gggccggcag gcccgcaccg ttcccgaatc gtgaccggac tcgaccaact cgatcagccg
      601 cgtcaggtgc tcggcgcggt agttctggta cacggcgtcg atctcggggt cggcgaacac
      661 atcgtcgaag aacgcggacc gggcctcgca ggcgacaacg ctgaccgcaa gatgggcaac
      721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      841 gcaccgactg tcgcggccca gaaccgtacg ttctcggcca cggtgagatc ggcgaatccc
      901 tcgacgacga cgatcgcgct gagctcgtcg aggatctcga gctgccgtcg cgagtacatc
      961 atcgtcacct cggcgacgtt accgaacgcc cgagtgacgt cacgggttcg ctcggtcgtc
     1021 cctgctcaat cgtcgaggtg gtagacgcgg gcagcgttgc cgtgcaacac cttgcgggtg
     1081 atctccagcg gaacgtcggc gaagtgcttc gcgatgtggt cctgcgggat ctcggcnnnn
     1141 nnnnnnaccg ggcgacatcg ccgtcgggtg cgggtagtcg gtctcgaaca tgaagttgtc
     1201 gggatacgtg tcgagcaacc gcagcgtcga ggtctcgaac cagaacgtgc catagcactg
     1261 ccgggcgaag tactcgctcg gcagcatgtc cgacttggtc tcgcgagacg ctccataacc
     1321 cttccagtgc cagtcgagcg agtcgagcag gtacggaatg aatccgaagc cgctctcgac
     1381 cgagacgaag ttgaccgttg ggtggcgctc gcaaaccccg gaggtgatga tgcgagcgat
     1441 cgaatcaccg ttgctcatca acccgatcgc cgtacccacg gcggcggccc gagccgagaa
     1501 gttggccaac atggccgtca tggcttgtgc cgagccgtcg gtgctggaac tgaacccgac
     1561 gtgatagttg accgacgtgt ccagttcggc gcacgtcttg tagatcgggt cccagtgcgt
     1621 gtcggtgaag gccggcaggc cgatctgctc gtacttgttg gcgaacagga tgccccggtg
     1681 acccatttcg tgacagcggc ggatctcctt ctccgactcc tcaaggtccc agaacgggac
     1741 catcgtgatc gggatgaagc gctttgggtc ggtgctcgcg aactcggtct ggaagtcgtt
     1801 gtagacctgt acgcaagcca gcgccgcgtc cagaccgagc ttcatgaact ggccggtctc
     1861 gaagccgatc aggttcgggt agagcacctg ggcgtagatg ccgtgctcgt ccatccgctg
     1921 cagacggacg ttggggtcgt acgaaccctt gtcaacctcg ggatgatcga gatcgtgggg
     1981 gccgttcggc gggaactcct tccagccggc gacggcatag aagccacttc ccatcagcca
     2041 gtaatcgccg acgcgccagt ggtcgtgacc agtcgcttcg ttgtgctgga cgcgcggcgc
     2101 gatgtcttga aacttcgcgg gaagccgcga cgtccacaag tcggccggct cactgatgtg
     2161 ggtgtcggta tcgatgacct tgaggtcctg aacgttgaga gtgtcagtca tagcattgcc
     2221 tttctggtga aatacctggc tggtgggcca cctcacgcaa gggaatcctg ttcaagtgga
     2281 cggaacnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc
     2341 cctctctggt accgtcaatc tccccggcaa tttctgcaac gtagactgga cgtgatggac
     2401 gagcatccga acgttgccgt gatccgagcg gcgctcgagc acgtcggcaa gggcgatatc
     2461 gatgccatgt tcgccttgtg ggagaacgag ttcgtctact acggcttcga tgcggaaggc
     2521 cgcaatcgag agtgccgtaa ccgcgccgac ttcaccgaga tcttcgggat cggccaacgc
     2581 ttgctggaga cccacgagaa cgaggtcatc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tcacacgcca acaacgtcgc cgacttcctg
     2701 atggtgttcc acgtccgcaa cggcaagatc cgcttcgcct gcgacttcat ggacacgtcg
     2761 acgcaagact tcctggacag cgcttggtcg tgagggtgac gcaacttgtc caacaaccca
     2821 ccgttcggta aaactgcaga catcatcggc gggggacgtt gggcacggtg agtcccgcag
     2881 tttcgacact cgtccgacga caaccttgaa aggaagaacc atgagtcgag aagcttggat
     2941 tatcgacgcc gtgcgatcgc cgcgaggcaa gggcaaggag ac
//
LOCUS       DAORNF010000409         2979 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_25054_length_2979_cov_1.590629, whole genome shotgun sequence.
ACCESSION   DAORNF010000409 DAORNF010000000
VERSION     DAORNF010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2979
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25054_length_2979_cov_1.590629"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1104)
                     /locus_tag="PLV68_14805"
     CDS             complement(<1..1104)
                     /locus_tag="PLV68_14805"
                     /inference="COORDINATES: protein motif:HMM:NF017669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HRE02568.1"
                     /translation="MADLLVGGQVDPATHQRTDALTTIASSDLTTHGVIVGMTGSGKT
                     GLGIVLIEDALAAGIPTLLIDPKGDLTNLCLTFPDLAPSDFEPWVNEGDAAKAGLSVT
                     DFAAAQAASWTKGLAGWNRGPADIAAFRQRVGLTIYTPGSSTGVGLNIVGSLTAPRDL
                     SDDEIVGDEIEGFVSGLLGLVGIDADPLASKEHILLSNLILNEWSNGRDLDLAALIGM
                     VQNPPIRKLGVFDLDAFFPAKERTALAVRLNGLLASPSFGAWMSGVALDIDAMLHLPD
                     GRPRAAIVCTAHLTDQERQFVTTLVLSKLITWMRRQSGTTDLRAMLYMDEVAGYLPPT
                     AMPPTKKPIMTLMKQARAFGVGVVLSTQNPVDID"
     gene            1208..1489
                     /locus_tag="PLV68_14810"
     CDS             1208..1489
                     /locus_tag="PLV68_14810"
                     /inference="COORDINATES: protein motif:HMM:TIGR02118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EthD family reductase"
                     /protein_id="HRE02569.1"
                     /translation="MIRVSVMYPAGDDTTFDHDYYKDSHVPKACAAWNVGAEIDKGIN
                     GPNVAAVHFFFDSLEQMQTAMATPATAEVMADVANYTNITPVMQISEVV"
     gene            complement(1473..2192)
                     /locus_tag="PLV68_14815"
     CDS             complement(1473..2192)
                     /locus_tag="PLV68_14815"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HRE02570.1"
                     /translation="MASPTAELTGAKRRIVETLKRAGLATATDLATRVGTTPAAVRQH
                     LDTLESAGLVVRDIAPVVPGAGSSEPGRPATQWRVTGLARAVFPDRHSDLTVDLLESI
                     RTTLGQQALDAVIDRRAALQIESYRDQIEAHRQQLPGDESLVARVRVLAERRSAEGYL
                     AEVVPSAGGDDGHRGDRDVLLIEHHCPISDAARTCPSLCRSELDVFRAALGPAVSVER
                     VQHQLAGDGRCSYRIGVRPLR"
     assembly_gap    2286..2298
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2364..>2663
                     /locus_tag="PLV68_14820"
     CDS             2364..>2663
                     /locus_tag="PLV68_14820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-5'-phosphate-dependent protein subunit
                     beta"
                     /protein_id="HRE02571.1"
                     /translation="MDDALVNQFGLADRIVEDAALANSVRRFREQGIRLPTFAQLASP
                     ASIDPALVAGVGPNDASPANLWRVHWYNDLAGGRVEVPDHVVLGPELTGVESPIIV"
     assembly_gap    2664..2772
                     /estimated_length=109
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2773..>2979
                     /locus_tag="PLV68_14825"
     CDS             <2773..>2979
                     /locus_tag="PLV68_14825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-5'-phosphate-dependent protein subunit
                     beta"
                     /protein_id="HRE02572.1"
                     /translation="PSTGNYARGGIAISRIMASRGVAILPAGMSQERFDWLDAWCVNP
                     AEDVIRTVGTESNVKEIYDACNQL"
BASE COUNT          457 a          947 c          994 g          459 t
ORIGIN      
        1 gtcgatgtcg accgggttct gggtgctgag caccaccccc acgccgaacg cgcgggcctg
       61 cttcatcagc gtcatgatcg gcttcttggt cggcggcatc gcggtgggcg gcaggtaccc
      121 ggcgacctcg tccatgtaca gcatcgcccg caggtcggtg gtgccactct ggcggcgcat
      181 ccaggtgatc agcttcgaca gcaccagcgt ggtgacgaac tggcgctctt gatcggtgag
      241 gtgcgcggtg cacacgatgg ccgcgcgggg ccgtccgtcg ggcaggtgca acatggcgtc
      301 gatgtcgagc gcgacgcccg acatccacgc cccgaacgac ggcgaggcga gcaggccgtt
      361 gagccgtacc gccaacgcag tgcgctcctt ggcgggaaag aacgcgtcga ggtcgaacac
      421 gccgagcttg cggatcggcg ggttctggac catgccgatc agcgcggcca ggtcgaggtc
      481 gcgcccgttc gaccactcgt tgaggatcag gttgctgagc aggatgtgct ccttgctggc
      541 cagcggatcg gcgtcgatgc cgaccaggcc gagcaggccg ctgacgaagc cttcgatctc
      601 gtcaccgacg atctcgtcgt cgctcaggtc gcgcggtgcg gtcaacgatc cgacgatgtt
      661 cagccccacg ccggtcgacg acccgggcgt gtagatggtg agccccactc gttggcggaa
      721 cgcggcgatg tctgccggac cgcggttcca accggcgagc cccttggtcc acgaggctgc
      781 ttgggccgcg gcgaagtcgg tgacgctgag accggccttg gccgcgtcgc cttcgttgac
      841 ccagggttcg aagtcggacg gggccagatc ggggaaggtg aggcacaggt tggtcaggtc
      901 gcccttcggg tcgatcagca gggtggggat gcccgccgcc agcgcgtctt cgatgagcac
      961 gattcccaga cctgtcttgc ccgacccggt catgccgacg atgacaccgt gggtggtgag
     1021 gtcggacgac gcgatcgtgg tcaatgcgtc ggtgcgttga tgagtggctg ggtcgacctg
     1081 gccgccaacg aggagatctg ccatggggcc acacattaat gaccacgtag agaatggtgt
     1141 ccgctccgtc acgtcggatc gcgccttcgc tccgcagtgg ccggccacga cgcgatagct
     1201 tccgaccatg attcgcgtga gcgtgatgta ccccgccggt gacgacacca ccttcgacca
     1261 cgactactac aaggacagcc atgtccccaa ggcctgtgcc gcatggaacg tgggtgccga
     1321 gatcgacaag ggcatcaacg gccccaacgt cgccgcggtg catttctttt tcgacagcct
     1381 cgagcagatg cagactgcca tggccacccc ggccacggcc gaggtgatgg ccgacgtggc
     1441 caactacacc aacatcactc cggtgatgca gatcagcgaa gtggtctgac accgatccgg
     1501 tagctgcagc ggccgtcacc ggccaactgg tgctggacgc gttcgacgct gacggcgggg
     1561 ccgagcgcgg cgcggaacac gtcgagctcg gaacggcaca ggctgggaca cgtacgcgcg
     1621 gcgtcgctga tggggcagtg gtgctcgatc agcagcacgt cgcgatcgcc acgatgacca
     1681 tcatcgccac ctgcgctggg caccacctcg gccaggtagc cctcggcgct gcgccgctcg
     1741 gccagcacgc ggacccgggc cacgaggctc tcgtcgcccg gcaactgctg gcgatgcgct
     1801 tcgatctgat cgcgatagct ctcgatctgc agagcggcgc ggcggtcgat cactgcgtcg
     1861 agcgcctgtt gaccgagggt ggtgcggatc gactcgagca ggtcgaccgt caggtcgctg
     1921 tggcggtcgg gaaagaccgc tctggccagg ccggtgacgc gccactgcgt ggcgggacgc
     1981 cctggctcgg acgagcccgc accgggtacg accggcgcga tgtcgcgcac gaccagcccg
     2041 gccgactcga gcgtgtcgag gtgttggcgc accgccgccg gggtggtacc gacccgtgtc
     2101 gccaggtcgg tggccgttgc cagcccggcg cgcttgagcg tttcgacgat gcggcgcttg
     2161 gcgccggtga gctcggcggt cggcgaagcc atggccgcca ggttaacgac cggcgaaagg
     2221 ggcccagacg ccgctgctcg cggtgcggtc tgctgcgccg aagcaggtgt gcggcttgac
     2281 agaatnnnnn nnnnnnnncc gacccagccg cccattcgtc gccgccatct gcaaccctcg
     2341 atcgaggcgg tgaggcgtcc gcgatcgacg acgcgctcgt caaccagttc gggctggccg
     2401 atcggatcgt cgaggacgca gcgttggcca actcggtacg gcgctttcgg gagcagggca
     2461 ttcgcctgcc caccttcgcc caactcgcca gcccggcttc gatcgatccg gcgctggtcg
     2521 cgggtgtggg gcccaacgac gccagccccg ccaacctgtg gcgggtgcac tggtacaacg
     2581 acctggccgg tggtcgggtc gaggtgcccg accacgtggt gctcggtccc gaactgaccg
     2641 gtgtcgagtc gccgatcatc gtcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnggcccagc accggcaact acgcccgcgg cggcattgcg atcagccgca
     2821 tcatggccag ccggggtgtc gccatcctgc ccgccggcat gagccaagag cgcttcgact
     2881 ggctcgatgc gtggtgtgtc aaccccgccg aggacgtgat ccgcaccgtc ggcaccgaga
     2941 gcaacgtcaa ggagatctac gacgcctgca atcagttgg
//
LOCUS       DAORNF010000410         2977 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_25069_length_2977_cov_1.567077, whole genome shotgun sequence.
ACCESSION   DAORNF010000410 DAORNF010000000
VERSION     DAORNF010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2977
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25069_length_2977_cov_1.567077"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2977)
                     /locus_tag="PLV68_14830"
     CDS             complement(<1..>2977)
                     /locus_tag="PLV68_14830"
                     /inference="COORDINATES: protein motif:HMM:NF015140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HRE02573.1"
                     /translation="DIDDCPRVQLNVSYGWLHVGAITTKYRDFLTDPDGGGPLQAGDE
                     NPADPVYQLSTGAVVDELYTTVDGSTLLGINGTQAQLNNTLKALKYKPPADYHYAGSG
                     TLNLLKVDIASGDPSVSPITGNDIEIRVLDVNDWPTLDGPTAAVNADPEQELVLTNQY
                     TVADEDNDEIIDGDQADPPEPEETYPDGDGDWMLLIGVLDCGAPTGNPADTGFHFKGG
                     SFVADQADIEDLLKDVFDLSGQPEIEAFIDAVLLGLETIKPGITTIPLATNNPFTFTQ
                     AFAGIADIQEVQYALDEVTFRSDAEMDSCDLITVVSDLGNNGLPLQYIGERPTGVEIP
                     IPGFDFNILTINVGELLEIDASFDPGSIFVHETETATARINIDPPTHPAFSLKVSALS
                     GTAISGDDFASLTDLTVDVPEDAAYVEVSTNAYGDSNAEGSENFTFVITTPTDPPPGS
                     FYRPFGYTVESARPVQNVVILDDDDAVNQVNIADASVVEGDGPGATNMEFTLSFTGGA
                     YADGDESVDIVLTPGTATSPADFGAASPSTVTFAPGATTATVTVPIVGDLIPESDETF
                     TVGLTGAQNVTIGDAQATGTIVDDDQPRIASVSDAMQNEGNGPGTTNMTFTISLDRPA
                     KAGDSVTVGTSDGTAVAPGDYTTVTTTLVFAAGETSKTVDVPIVGDADIEPNETFILA
                     ITGTSSAEVQAGDGAGTGTIVNDDGVPAVSIGDVTMAEGHSGSTTMTFTLSLSAAALG
                     GEMVTVATADNTATGGSDYVALVPTAVTFDEDDMTKIVSVTINGDTAVEPDETLFVNL
                     SNPLGLTIADAQGIGTITNDDISVTIDPASTSVAEGGTANLTITIAPAVHDAFTIDVL
                     TTNGTAVAPGDYTAVSITPQTVAANATTIPLAITTIDNAISDGTRSFTVNGSNPTPPS
                     GFGVTFGPAATVLITDDEGPADNTPPSVTVVPAAGQESPTNDTTIHFTVTFSAATGLA
                     NVTLTNTAGREIALDYTPPH"
BASE COUNT          423 a          896 c         1073 g          585 t
ORIGIN      
        1 gtggggcggc gtgtagtcga gggcgatctc gcgccccgct gtgttggtga gcgtcacgtt
       61 cgctagtccc gtggcggcgg agaacgtcac ggtgaagtgg atcgtcgtgt cgttggtcgg
      121 tgactcctgc ccggcggcgg gtaccaccgt caccgagggc ggcgtgttgt cggccggtcc
      181 ctcgtcgtcg gtgatcagca cggtggctgc cgggccgaag gtgacgccga atccgctggg
      241 tggcgtggga ttcgacccgt tgaccgtgaa gctacgtgtg ccgtcggaga tcgcgttgtc
      301 gatggtggtg atcgccaacg ggatcgtggt cgcgttggcg gcaaccgtct ggggcgtgat
      361 gctgaccgcg gtgtagtcgc ctggggcgac ggcggtgccg ttcgtcgtga gcacgtcgat
      421 ggtgaacgcg tcgtgtaccg ctggagcgat cgtgatcgtg aggttggcgg tgccaccctc
      481 ggcgaccgac gtcgaggccg ggtcgatcgt gacggagatg tcgtcgttgg tgatcgtgcc
      541 gatgccttga gcatcggcga tggtgaggcc gagcgggttc gacaggttga cgaacaacgt
      601 ctcgtcgggc tcgacggccg tgtcgccgtt gatcgtgacc gacacgatct tggtcatgtc
      661 gtcctcgtcg aaggtgaccg ccgtcggcac cagcgccacg tagtccgagc ctccagtggc
      721 ggtgttgtcg gccgtcgcca cggtgaccat ctcaccgccg agggcagcgg cggacaacga
      781 cagtgtgaac gtcatcgtcg tcgaccccga gtgcccctcg gccatggtga cgtcgccgat
      841 cgacaccgcc ggcacgccat cgtcgttgac gatcgttccg gtgcccgcgc cgtcaccggc
      901 ttggacctcg gccgacgagg taccggtgat cgcaaggatg aacgtctcgt tcggctcgat
      961 gtcggcgtcg cccacgatcg gcacgtcgac cgttttggac gtctcaccgg cggcgaacac
     1021 cagcgtggtg gtcacggtgg tgtaatcccc cggcgcgact gcggtgccat cggaggtacc
     1081 gaccgtgacg ctgtcaccag ctttggccgg ccggtcgagc gagatcgtga acgtcatgtt
     1141 ggtcgtaccg gggccgttgc cttcgttctg catcgcgtcg gacacggacg cgatgcgtgg
     1201 ctggtcgtcg tcgacgatcg tgccggtggc ctgcgcgtcg ccgatggtga cgttctgggc
     1261 gccggtgagc ccgacggtga acgtctcgtc gctctccggg atcagatctc cgacgatcgg
     1321 cacggtgacg gtggccgtcg tcgctcccgg agcaaacgtc accgtcgacg gcgaggctgc
     1381 gccgaagtcg gcggggctgg tcgccgtgcc aggggtgagc acgatgtcga cgctctcgtc
     1441 accgtcggcg taggcgccgc cggtgaagct gagcgtgaac tccatgttgg tcgcgcccgg
     1501 gccgtcacct tcgaccaccg acgcgtcggc gatgttgacc tggttcaccg catcgtcgtc
     1561 gtcgaggatg accacgttct gcaccggccg tgcggactcg accgtgtatc cgaagggtcg
     1621 gtagaacgag ccgggcggcg ggtcggtcgg ggtggtgatc acgaacgtga agttctcact
     1681 gccttcagcg ttggagtcgc cgtaggcgtt ggtcgagacc tcgacgtagg cggcgtcctc
     1741 gggcacgtcg accgtcaggt cggtcagcga ggcgaagtcg tcaccggaga tcgccgtgcc
     1801 gctcagcgcc gacaccttca ggctgaacgc cgggtgggtc ggcggatcga tgttgatgcg
     1861 agctgttgcg gtctcggtct cgtggacgaa gatgctgccg ggatcgaaac tggcgtcgat
     1921 ctccaacaac tcgccgacgt tgatcgtcaa gatgttgaag tcgaatccgg ggatcgggat
     1981 ctcgacaccg gtcgggcgct cgccgatgta ctgcagcggc aggccgttgt tgcccaggtc
     2041 ggacaccacg gtgatcagat cgcacgagtc catctcggcg tcggagcgga acgtcacttc
     2101 gtcgagcgcg tactgcacct cctggatgtc ggcgatgccg gcgaaggcct gggtgaaggt
     2161 gaacggattg ttcgtcgcca gcggaatcgt cgtgatcccc ggcttgatcg tctccagacc
     2221 gagcagcacc gcgtcgatga acgcctcgat ctcgggctga ccgctcaggt cgaacacgtc
     2281 cttcagcagg tcttcgatgt cggcctggtc ggcgacgaag ctgccgccct tgaagtggaa
     2341 tccggtgtcg gccgggttgc cggtgggcgc gccgcaatca agcacgccga tgagcaacat
     2401 ccagtccccg tcgccgtcgg gatacgtctc ttcgggttcg ggcggatcgg cctgatcgcc
     2461 gtcgatgatc tcgtcgttgt cctcgtcggc gaccgtgtac tggttggtca gcaccagttc
     2521 ttgttcgggg tcggcgttga ccgctgcggt cgggccgtcc aacgtcggcc agtcgttgac
     2581 gtcgagcacc ctgatctcga tgtcgttgcc ggtgatcggg gagacgctcg ggtcgcccga
     2641 cgcgatgtcg accttgagca ggttgagcgt gcctgacccg gcgtagtggt agtccgctgg
     2701 cggcttgtac ttcagcgcct tgagcgtgtt gttgagctgg gcctgggtgc cgttgatgcc
     2761 gagcagcgtc gagccgtcga ccgtggtgta gagctcgtcg acgacagcgc ccgtcgagag
     2821 ctggtacacc ggatccgccg ggttctcgtc gccggcctgc agcgggccac caccgtccgg
     2881 gtcggtgagg aagtcgcgat atttcgtggt gatcgcaccg acgtggagcc atccgtagga
     2941 gacgttgagc tgcacccgcg ggcagtcgtc gatgtcg
//
LOCUS       DAORNF010000411         2958 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_25291_length_2958_cov_1.725112, whole genome shotgun sequence.
ACCESSION   DAORNF010000411 DAORNF010000000
VERSION     DAORNF010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2958
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25291_length_2958_cov_1.725112"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>868
                     /locus_tag="PLV68_14835"
     CDS             <1..>868
                     /locus_tag="PLV68_14835"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRE02574.1"
                     /translation="VAVDARDLARKCGATVVEVWALNLMGIVHFNAGNHSDALSCCLR
                     GLELYRSTDHQIDEGNLLNTIAAIHHELGDTDRAIVTYEAALSANRQLERPDFDAVTL
                     NNMAQLRAERREFFLAASLGESSLELSRQHAPGFVPEVLASLGEAYTNLSDERRSTEC
                     FAEALQILDDRSQRGIETSPTAVIAVRLARGKSALTSGELDIAERDLLTALDVARRAS
                     IRSQELKAHELLATVYKRLGRFEQALAHQEARFELHQDLFNQGTDLRIKTLQIAHDTE
                     HARQQAEILRLRT"
     assembly_gap    869..1046
                     /estimated_length=178
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1047..1521
                     /locus_tag="PLV68_14840"
     CDS             <1047..1521
                     /locus_tag="PLV68_14840"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRE02575.1"
                     /translation="DARLHDIGKVAVPDAILLKPGPLSVDEFEVMKTHTTIGAQILSG
                     STSSLIQLGAVVALNHHERWDGTGYPAGLSGTDIPRSGRIVTVADVFDALTSVRVYKH
                     AWSQADALAYIAGARGTQFEPEVVDAFIRVITRRIPALRSAPIDTRIPAPTRQLH"
     gene            1531..>1810
                     /locus_tag="PLV68_14845"
     CDS             1531..>1810
                     /locus_tag="PLV68_14845"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HRE02576.1"
                     /translation="MPLSAVVASIPSPSSGSIGIGPLDIHAYGLMIALGVVAAVMITG
                     RRFEQKGIGTRDDVNSMAVWAVLAGVVGSRLYHVITDWSKFEDNLGDIP"
     assembly_gap    1811..1828
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1829..2526
                     /locus_tag="PLV68_14850"
     CDS             <1829..2526
                     /locus_tag="PLV68_14850"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HRE02577.1"
                     /translation="GIPGGLIFGIGVGVWYFKRKGIPVGLAVNSAAPAIAVAQAIGRW
                     GNYFNQELYGRVTTLPWGLEIDDAKRTGDAAQYVGKDVLFHPTFLYESLANLALAAGL
                     IVLDRKRQVRPGHLLAMYIVGYGVIRFFVEGLRIDEADHVAGLRWNQWMALAAVVGGL
                     IYLAVSSRRPLPAADEGAEIAETASPEAGSAEIAEDIDDIDDIDGSDIEDIDIQGGTT
                     ESDAASLDDEPEA"
     gene            2537..>2958
                     /gene="gatC"
                     /locus_tag="PLV68_14855"
     CDS             2537..>2958
                     /gene="gatC"
                     /locus_tag="PLV68_14855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HRE02578.1"
                     /translation="MSERITTSDVVKVAKLARLDLDPSQVERTTAQLAGMLEHFADID
                     ALDLSEVEPMTQPYPLTNVFSADVVAASLDRDRRRPLPGATNSGTRRMSAAESPTLSA
                     LEIAAGVRAGTLKAADVLEQHLARIEAREPDVHAFNLV"
BASE COUNT          472 a         1015 c          866 g          409 t
ORIGIN      
        1 ggtcgcggtc gatgcccgcg atctcgcccg caagtgcggc gccaccgtgg tcgaggtgtg
       61 ggcgctcaac ctgatgggca tcgtccactt caacgccggc aaccactccg acgccctgtc
      121 gtgctgcttg cgcggcctcg agctctaccg cagcaccgat caccagatcg acgagggcaa
      181 cctgctcaac acgatcgccg cgatccatca cgagctcggc gataccgacc gggccatcgt
      241 cacctacgaa gcggcgttgt cggccaaccg ccagctcgag cgacccgact tcgacgcggt
      301 cacgctcaac aacatggccc agttgcgcgc cgagcggcgc gagttcttcc tggccgccag
      361 cctcggcgag tcgtcgctcg agctgtcacg ccaacatgcg cccggcttcg tgcccgaggt
      421 gctcgccagc ctgggcgagg cctacaccaa cctcagcgac gagcgacgct ccaccgagtg
      481 cttcgccgag gcgctgcaga ttctcgacga ccgcagccag cgcggcatcg agaccagccc
      541 caccgccgtc atcgcggtgc gcctggcccg tggcaagtcg gccctcacca gcggcgaact
      601 cgacatcgcc gagcgcgacc tgctgacggc gctcgacgtg gcccgccggg caagcatccg
      661 ctcccaagag ctcaaggccc acgagctgct cgccacggtg tacaagcgcc tcggccgctt
      721 cgaacaagcc ctcgcccacc aagaggcacg cttcgaactg caccaggacc tgttcaacca
      781 gggcaccgac ctgcggatca agacgctgca gatcgcgcac gacaccgagc acgcccgtca
      841 gcaggccgag atcttgcggc tgcgcaccnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1021 nnnnnnnnnn nnnnnnnnnn nnnnnnggac gcccgcctgc acgacatcgg caaggttgcc
     1081 gtgcccgacg cgatcttgct caagcccggc ccgctcagcg tcgacgagtt cgaggtgatg
     1141 aagacgcaca ccacgatcgg ggcgcagatc ctgtcgggga gcacgtccag cttgatccaa
     1201 ctcggtgcgg tcgtcgcctt gaaccaccac gagcgctggg acggcaccgg ctatccggcc
     1261 ggtttgagcg gcaccgatat cccccgcagc ggccggatcg tcaccgttgc cgacgtgttc
     1321 gacgcgctca ccagcgtgcg ggtctacaag cacgcgtggt cgcaggccga tgcgctggcg
     1381 tacatcgccg gcgcccgcgg cacgcagttc gaacccgagg tcgtagacgc cttcatccgg
     1441 gtgatcaccc gccgcatccc ggctctgcgc agcgcgccga tcgacacacg cataccagcg
     1501 cccacacgcc agctgcacta gaacaggcgc atgcccctca gcgccgtcgt ggccagcatt
     1561 cccagtccct ccagcgggtc gatcgggatc gggccgctcg acatccatgc ctacggtctg
     1621 atgatcgcgc tcggcgtcgt cgccgccgtg atgatcaccg gtcgccggtt cgagcagaag
     1681 ggcatcggca cccgtgacga cgtcaactcg atggcggtgt gggcggtgct cgccggcgtc
     1741 gtcgggtcgc ggctgtatca cgtgatcacc gactggtcga agttcgaaga caacctcggc
     1801 gacatcccca nnnnnnnnnn nnnnnnnntg ggcatccccg gtgggttgat cttcggcatc
     1861 ggcgtcggcg tctggtactt caagcgcaag ggcattcccg tgggcctcgc cgtcaactcg
     1921 gccgcccccg cgatcgcggt cgctcaggcg atcggccgct ggggcaacta cttcaaccag
     1981 gagctgtacg ggcgcgtcac gaccttgccc tggggtctgg agatcgacga cgccaagcgc
     2041 accggtgacg cggcccagta cgtgggcaag gacgtgctgt tccaccccac gttcctgtac
     2101 gagtcgctgg ccaacctcgc cctggcagcg ggtctgatcg tgctcgatcg caagcgccag
     2161 gtccgccccg gacacctgct ggcgatgtac atcgtgggtt acggcgtgat ccgcttcttc
     2221 gtcgaagggc tgcgcatcga cgaggccgac catgtcgccg ggctgcgctg gaaccagtgg
     2281 atggccttgg ccgccgtcgt cggcgggctg atctacctcg ccgtctcgtc acgccgcccg
     2341 ctcccggcgg ccgacgaggg cgccgagatc gccgagaccg ccagtcccga ggctggcagc
     2401 gccgagatcg ccgaggacat cgacgacatc gacgacatcg acggcagcga catcgaggac
     2461 atcgacatcc agggcggcac gaccgagagc gacgcagcaa gcctggacga cgaaccagag
     2521 gcctagcctg gcaggcgtgt ctgaacggat cacgacctcc gacgtggtga aggtggccaa
     2581 gctggcccgc ctcgacctcg acccctccca ggtcgagcgc accaccgccc aactcgccgg
     2641 catgctcgaa cacttcgccg acatcgacgc gctcgatctc agcgaggtcg aaccgatgac
     2701 gcagccctac ccgctgacca acgtgttcag cgccgacgtc gtggctgcca gcctcgatcg
     2761 cgaccgaaga cggccgcttc cgggtgccac caattctggg actcgacgga tgagcgccgc
     2821 ggagtcgccc accctgtccg cgctagagat cgctgcgggt gtgcgggcgg gcacgctcaa
     2881 ggctgctgac gtgctcgaac agcacctcgc ccgcatcgaa gcccgcgagc ccgacgtgca
     2941 cgcgttcaac ctcgtcca
//
LOCUS       DAORNF010000412         2956 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_25319_length_2956_cov_2.037573, whole genome shotgun sequence.
ACCESSION   DAORNF010000412 DAORNF010000000
VERSION     DAORNF010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2956
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25319_length_2956_cov_2.037573"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>220)
                     /locus_tag="PLV68_14860"
     CDS             complement(<1..>220)
                     /locus_tag="PLV68_14860"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02579.1"
                     /translation="VVLTDTVPANTTFVSASDGGAESAGTVTWPAVSLAGNGATFTRT
                     LTVQVDDPLAAGVTTIDNTALVGDDGASG"
     assembly_gap    221..231
                     /estimated_length=11
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<232..>1388)
                     /locus_tag="PLV68_14865"
     CDS             complement(<232..>1388)
                     /locus_tag="PLV68_14865"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HRE02580.1"
                     /translation="PSVSGGASDGDDIVFTFNDPVTVTSDNDPNNDTFTLTFQARVLD
                     TAPNQNGQTRVNGATVAADGVVLATNTVTVTVVEPDLAVNVTVDDASAGFGQILTYTL
                     DVSHTVASTSPAEDVVMTVTIPAGLTYVPGSVTPPPGWSVDDSTPGQLVFTIAEWTTG
                     AIAPTFQAVVGDSPVVNIGDVLTVPVDLTWTSLPGVDAGERTGIGGVDDYARSDSVDV
                     TVSGIDLQIVKTDADITSAPGGLITYTLTITNTGNTPATGVVITDSVPANTSFVSANL
                     GGVESAGTVTWSVGSLGVGASASRQLVVQVDNPVPVGLTAISNTALVGDDGATGPDLN
                     PADNSDTEPTPIGGVVIDLQLTKDDGVASATPGQTLVYSLVVTNVGTTTATG"
     assembly_gap    1389..1409
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1410..>2956)
                     /locus_tag="PLV68_14870"
     CDS             complement(<1410..>2956)
                     /locus_tag="PLV68_14870"
                     /inference="COORDINATES: protein motif:HMM:TIGR01451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02581.1"
                     /translation="TYRIAYTNSAAALAPAFDLNLTDTLPALMVGPFTITTSTTSGVC
                     GSLSPTFTTNVVGQDVTSALTCLTPGASAVVTITGQVASTAPVSSTLANTANLVSSTL
                     PGTNGTAPNPTDSTTPGAPGATNGEAVANTSSNVNVNLARPALDKLQPSPAAYTIGDV
                     ITYTIVITTPEGVTPNLVVTDTLPTGLSFVSRQVVTSAPLSNGLLSSDYGGSVIATPA
                     IANSGSAVSFTFGDLSNAADNDPANNRFLLVVLARVDNVIGNQAGDLLVNTANLSFGD
                     GTPSGTTTVNDPVGGTIALVEPILALSKTILTPPTPPDGAGTVTYQIVFNHVAGSGST
                     AYDTVLTDAIPAGLTNASVAGVIASGVAAPSAAVNAGVLQVPDGGALNLPVGAVVTVT
                     LQADLTATVAPGQVITNTGVLVWSTQPGVNANERGSGDSLLNAGGLNDYEVRSSVGLT
                     VDSPEVAKRLVTTSAPNTSGANVTIGELVTYAIDVTLPEGVTSALVVTDSLPSGLIYV
                     PASAGFD"
BASE COUNT          412 a          909 c         1099 g          504 t
ORIGIN      
        1 ccctgaggcg ccgtcgtcac cgaccagcgc ggtgttgtcg atcgtggtca cgcccgcggc
       61 gagcgggtcg tcgacctgca cggtgagcgt gcgcgtgaac gtcgcgccgt ttccggccag
      121 cgagacagcc ggccatgtga cggtgcctgc gctctcggcc ccgccatcgg aggctgagac
      181 aaaggtcgtg ttggccggga ctgtgtcggt gagcaccacg nnnnnnnnnn nccggtggcg
      241 gtggtggtgc cgacgttggt cacgaccagg ctgtagacca gggtctggcc cggggtcgcg
      301 ctggcgacgc catcgtcctt ggtcagctgc aggtcgatca ccacgccgcc gatcggcgtg
      361 ggctcggtgt cgctgttgtc ggccggattg agatccggcc ctgtcgcgcc gtcgtcgccg
      421 acgagcgctg tgttactgat cgcggtcagg cccaccggca ccgggttatc gacctgcacg
      481 accagctggc ggctggccga ggcgccgacg cccaggctgc cgaccgacca ggtgaccgtg
      541 ccggcgctct cgacgccgcc gagattggcg ctgacgaagc tggtgttggc cgggacgctg
      601 tcggtgatga cgacgcccgt ggccggggtg tttcccgtgt tggtgatggt gagcgtgtac
      661 gtgatcaggc cgccgggcgc gcttgtgata tcggcatctg tcttgacgat ctgcaggtcg
      721 atgcccgaga ccgtcacatc gacgctgtca gaccgcgcgt agtcgtcgac gccgccgatc
      781 ccggtgcgct cgccggcatc gacgccgggc agcgaggtcc aggtcaggtc gaccggcacc
      841 gtgagcacgt cgccgatgtt gacaaccggg ctgtcgccca ccacggcctg gaacgtcggc
      901 gcgatggcgc ccgttgtcca ttcggcgatg gtgaacacca actggccagg cgtcgagtcg
      961 tcgacgctcc agccgggcgg cggggtcacc gagccgggga cgtaggtcag gccggccggg
     1021 atcgtcacgg tcatcaccac gtcctccgcg ggcgaggtgc tggcgaccgt gtgcgagaca
     1081 tcgagcgtat aggtcaggat ctggccaaag cccgcgctgg cgtcgtcgac cgtgacgttc
     1141 acggcgaggt cgggctcgac cacggtcacg gtgaccgtgt ttgtcgcgag caccacgccg
     1201 tcggccgcga cagtcgcgcc gttaacgcgg gtctggccgt tctggttggg cgcggtgtcg
     1261 agcacgcgcg cctggaaggt caacgtgaag gtgtcgttgt tcggatcgtt atccgatgtc
     1321 acggtgaccg gatcgttgaa ggtgaagacg atgtcgtcgc cgtcgctggc gccgccgctg
     1381 acgctcggnn nnnnnnnnnn nnnnnnnnnc gtcgaagccc gcgctggccg gcacatagat
     1441 caggccggag ggcagcgagt cggtgaccac cagcgccgac gtcacgcctt cgggcagcgt
     1501 cacgtcgatg gcgtaggtca ccagctcgcc gatcgtaacg tttgcgccgc tggtgttcgg
     1561 ggccgaggtg gtcacgagac gcttggcgac ctcggggctg tcgacggtca ggccaacgct
     1621 cgagcgcacc tcgtagtcgt tgagaccgcc ggcgttgagc aggctatcac cgctgccacg
     1681 ttcgttggca ttgacgccgg gttgcgtgga ccagaccagg acgccagtgt tggtgatcac
     1741 ctggccgggc gccacagtcg cggtcaggtc ggcctggagc gtcacggtga cgacagcgcc
     1801 gacggggagg ttgagcgccc cgccatcggg cacctggagc acaccggcgt tgaccgccgc
     1861 cgagggcgcc gcgacgccgc tggcaatcac gccggcgacg ctggcgttgg tcaacccggc
     1921 cgggatcgcg tcggtcagca cggtatcgta ggccgtgctg ccgcttcctg cgacgtggtt
     1981 gaacacgatc tggtaggtca cggtgccggc gccatcgggc ggcgtcggcg gcgtgaggat
     2041 ggtcttgctc agggccagga tcggctcgac gagcgcgatc gtgccgccca cggggtcgtt
     2101 gaccgtggtc gtgccggagg gcgtcccatc gccgaagcta aggttggccg tgttgaccag
     2161 cagatcgccg gcctggttgc cgatcacgtt gtcgacgcgg gccagcacga ccaggaggaa
     2221 gcggttgttg gccgggtcat tgtcggcggc gttggagaga tccccgaacg tgaagctcac
     2281 ggcgctaccg ctgttggcga tggcgggcgt ggcgatcacg ctgccgccgt aatcgctcga
     2341 gagcagcccg ttgctcaacg gggctgaggt gacgacctgg cgcgagacga agctcagccc
     2401 ggtcggcagc gtatcggtca ccacaaggtt gggcgtgacg ccctcgggtg tggtgatcac
     2461 gatcgtgtag gtgatcacgt cgccgatcgt gtaggcggcc ggcgagggct ggagcttatc
     2521 cagcgccgga cgcgccaggt tgacgttgac gttagagctg gtgttggcga cggcctcgcc
     2581 gttcgtagcg ccgggcgcgc cgggcgtcgt cgagtcggtc gggttcggcg ccgtgccatt
     2641 tgtgccgggc agggtcgacg agacaaggtt cgcggtgttg gccagcgtgc tgctgactgg
     2701 cgccgtcgag gcaacctggc ccgtaatcgt caccacggcg ctggcgccgg gcgtgagaca
     2761 ggtcagcgcg cttgtgacgt cctggccgac gacgttggtc gtgaaggtcg ggctcaggct
     2821 gccgcagacg ccgcttgtgg tgctggtcgt gatcgtgaac gggccgacca tcaacgccgg
     2881 cagcgtatcg gtcaggttca ggtcgaaggc cggcgcgagc gcggcagccg agttcgtgta
     2941 ggcgatgcga taggtc
//
LOCUS       DAORNF010000413         2941 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_25464_length_2941_cov_1.902633, whole genome shotgun sequence.
ACCESSION   DAORNF010000413 DAORNF010000000
VERSION     DAORNF010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2941
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25464_length_2941_cov_1.902633"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            36..920
                     /locus_tag="PLV68_14875"
     CDS             36..920
                     /locus_tag="PLV68_14875"
                     /inference="COORDINATES: protein motif:HMM:NF024260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRE02582.1"
                     /translation="MADIDRLDALLEHFRVRTRLFHTGSLCGVTHFPAQPGRGFLHVF
                     RRGDMDVAHHDADGTLRRQRVDRPSLIFCPRAVDHSFDNAPDDDSDFACATFDIDGGA
                     THPLLDALAPLVVVPLDTAGDLDQALGLLFAEIDRVRCGNRVLADRLFEVLLIQLFRW
                     LLDHPDQLSVPAGLLAGLAEPRLARALTAIHESPGTPWTLSSMASRAGMSRSTFAAQF
                     KQLVGQTPAAYLTGWRITLAQAQLRAGNSVTRTAFDLGYATAPAFTRAFTARLGCSPR
                     AWLKTTGTDVSGAGTPAA"
     gene            complement(969..2174)
                     /locus_tag="PLV68_14880"
     CDS             complement(969..2174)
                     /locus_tag="PLV68_14880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HRE02583.1"
                     /translation="MASDDIFILGINMTKFGKHTDKDTVDLAAQATMAALADGGVSMR
                     DMGVLAAGCLMGGGGIAQQLQKQVGQTGIPAYNVANACATGATALRVAIMSIKAGECD
                     MGLAVGVEKLSGAGLLGGGGRAKQDGDGWSRSGRYGAVGPTDGRIGTDTMPGVFAQVG
                     MQYAHQYGGVDFDTFARISEKNHAHSTLNPLAAYQKRFTLDEIKNDIMISYPNTRPMC
                     SANCDGAAAAVVVSGAKLKTLSLEQQRRALKVSASVLTTDPWQEACQVLPDVNTLTRR
                     AATQAYELSGVGPDDLDLVELHDCFATAELVHYDNLMLCAPGEAGAFFDSGATWRDGK
                     TPVNVSGGLQSKGHPIAATGIANIWEVCHHLRGEAGDRQIEGAKVGLAHVIGLGSACG
                     VHILEKSAA"
     gene            complement(2288..2680)
                     /locus_tag="PLV68_14885"
     CDS             complement(2288..2680)
                     /locus_tag="PLV68_14885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HRE02584.1"
                     /translation="MTEQIPFVEYLHLGERPHLVASECTSCGARFFDRSNACASCFGT
                     EFRPVDIATDGIVTAFTIVALAAPGVAVPFVSAIVDCDGTSVRANLTNVDPTPEAVTL
                     GMPVRLTTVPVGADTNGVEAINFAFEPA"
BASE COUNT          424 a          995 c         1025 g          497 t
ORIGIN      
        1 caagaggccc cgtcgtgtcg tctagggtgc gcgccgtggc cgatatcgac cgactcgacg
       61 ctctgctcga acacttccgg gtgcggaccc gtctgttcca caccggttcc ctgtgcggag
      121 tcacccactt tccggcccag cccggtcgag ggttcctgca cgtgttccgc cgcggcgaca
      181 tggacgttgc ccatcacgac gcggacggca ccctgcgccg tcaacgggtc gatcggccga
      241 gcctcatctt ctgcccgcgg gccgtcgacc actccttcga taacgctccc gacgacgatt
      301 ccgacttcgc ttgcgccacc ttcgacatcg acggtggggc gacccacccc ttgctcgacg
      361 cgctggcgcc gttggtcgtg gtgccgctcg acaccgcggg cgatctcgac caagccctcg
      421 gcctgctgtt cgccgagatc gaccgagtcc gctgtggcaa tcgtgtgctc gccgaccggc
      481 tgttcgaagt gctcttgatc cagctgttcc ggtggctgct cgaccacccg gaccagttgt
      541 cggttcccgc aggactgttg gccggtctgg ccgagccgcg cctcgcccgt gccctcaccg
      601 cgatccacga gtcgcccggc acgccgtgga cgctgtcgtc gatggccagc cgggcgggca
      661 tgtcgcgcag cacctttgcg gcccagttca agcagctagt tggccagacc cccgcggcct
      721 atctgaccgg gtggcggatc accctggccc aggcgcaact gcgcgcgggc aactccgtga
      781 cgcgcaccgc cttcgacctg ggctatgcga cagcaccggc cttcacgcgg gccttcaccg
      841 cccggctcgg ctgctcacct cgcgcctggc tcaagaccac cggaacagac gtgtcgggcg
      901 cgggtacacc tgctgcctga cgcgccctga cgttgcggca cgcggcctgc gcccggggga
      961 gcggtgccct acgcggcgga cttctccagg atgtggacgc cgcaggccga accgaggccg
     1021 atgacgtggg ccaagccgac cttggcgcct tcgatctggc gatcgccagc ttcgccgcgc
     1081 aggtggtggc acacctccca gatgttggcg atgccggtcg cggcgatggg gtggcccttg
     1141 ctctgcaacc cgccggacac gttgaccggg gtcttgccat cacgccaggt ggcgccgctg
     1201 tcgaagaagg cacctgcctc gcccggcgcg cacagcatca ggttgtcgta gtgcaccagc
     1261 tcggccgtgg cgaagcagtc gtgcagctcg accagatcga gatcgtcggg gccgacaccg
     1321 gacagctcgt aggcctgcgt cgccgcccgg cgggtcaacg tgttgacgtc gggcagcacc
     1381 tggcacgcct cttgccacgg gtcggtggtc agcaccgagg ccgacacctt gagcgcccgc
     1441 cgctgctgtt cgagcgagag cgtcttcagc ttcgctcccg acaccaccac ggccgccgcg
     1501 gcaccgtcgc agttggccga gcacatcggg cgggtgttgg ggtaggagat catgatgtcg
     1561 ttcttgatct cgtcgagcgt gaaccgcttc tgatacgcgg ccagcgggtt gagcgtgctg
     1621 tgggcgtggt tcttctcgct gatgcgggcg aaggtgtcga agtcgacgcc gccgtactgg
     1681 tgcgcgtact gcatgccgac ctgggcgaac acgccgggca tggtgtcggt gccgatccgc
     1741 ccgtcggtcg ggccgactgc gccgtagcga ccggagcggc tccagccgtc gccgtcctgc
     1801 ttggcccgac cgccgccgcc gagcaaacca gcgcctgaga gcttctcgac gccgacggcg
     1861 agacccatgt cgcactcgcc cgccttgatc gacatgatcg cgacgcgcaa tgcggtggcc
     1921 cccgtggcac aggcgttggc cacgttgtag gcggggatgc cggtctggcc gacctgcttc
     1981 tggagctgct gggcgatacc gccaccgccc atcaggcagc cggccgcgag cacgcccatg
     2041 tcgcgcatgg acacaccgcc gtcggcgagg gcggccatcg tggcctgcgc cgcgagatcg
     2101 accgtgtcct tgtcggtgtg cttgccgaac ttggtcatgt tgattccgag gatgaagatg
     2161 tcgtcgctgg ccatgatgac tccgaatcgg tggtgcccga ggtgtgggcg agggtgaatg
     2221 gtgacgggaa cggctggttg gttgggccga ttggcggccg agatggtgga ccggttggtg
     2281 gcgcgggtca ggcgggctcg aaggcgaagt tgatcgcttc gacgccgttg gtgtcggctc
     2341 cgacgggcac cgtggtgaga cgcacgggca tgccgagcgt gaccgcttcg ggggtcggat
     2401 cgacgttggt caggttggcg cgcacgctgg tgccgtcgca gtcgacgatc gccgacacga
     2461 acggcacggc gacgccgggc gcggcgaggg cgacgatggt gaacgcggtg acgatgccgt
     2521 cggtggcgat gtcgaccggg cggaactcgg tgccgaagca cgacgcgcaa gcgttgctcc
     2581 ggtcgaagaa ccgggcaccg cacgatgtgc actcgctggc gaccaggtgc gggcgttcac
     2641 ccaagtggag gtactcgacg aaaggaatct gctcggtcac cccaccagtc ttgaccccgt
     2701 gccatgtgcg tgacgaatcg gccgaccgat ccgcgttcgt cccagaccgc gtcaaccgac
     2761 ccagatggag tcgccgcagc cggtgcgcct ggtcagggct gggtgaaggc gacgagctcg
     2821 atcatcacgt cgggggcgtc ttggaaggcg agcagaccgt tggacagcgc gcagtaggtc
     2881 ttgttgccac gggcgaagtt gacctgcacg cacgtcgaga cctggccggc agcctcgcgg
     2941 g
//
LOCUS       DAORNF010000414         2934 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_25544_length_2934_cov_1.914554, whole genome shotgun sequence.
ACCESSION   DAORNF010000414 DAORNF010000000
VERSION     DAORNF010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2934)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2934)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2934
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25544_length_2934_cov_1.914554"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(52..714)
                     /locus_tag="PLV68_14890"
     CDS             complement(52..714)
                     /locus_tag="PLV68_14890"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HRE02585.1"
                     /translation="MPRPDAPFDTVIFDLDGTISDSAPGILASLDHAFTQTGYPPPPN
                     LRRFIGPPFETAFRAEGFSPDEVKTLISVYREHYWDIGVFENFVYPGMEALLDGLAAD
                     GFRIAIGTSKPEPTARRILEHWGYTGRFDVIGGASFDMTRSTKAAVLAYVLEELGQCR
                     PVMIGDRYHDVEGSAQFGIDCVGVRWGYAASDDELERAGARWIVDHPNEIFDIVRGTL
                     LL"
     gene            complement(761..1564)
                     /locus_tag="PLV68_14895"
     CDS             complement(761..1564)
                     /locus_tag="PLV68_14895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRE02586.1"
                     /translation="MNGGLVLRIMPPVVVNSRRPQRMVERTMMCYRNTWPILISGFFE
                     PLFYLLSIRIGVSKLVGDVTVGGRTIGYAEFVAPALLAASAMNGAVYDSTMNVFHKLR
                     YAKTYDAILATPMTVGDVALGEILWALIRGFLYSAAFLVTMIPFGLVRSGWMVLSLPA
                     AVLIGFAFASVGMAATTYMRSWSDFEWVQTATMPLFLFSATFYPLSSYGDWGWVVQLS
                     PLYHGVALVRAANTGEATWAIAGHVAFLLTMCVAGLWVVSRRLQVLLLK"
     gene            complement(1561..2352)
                     /locus_tag="PLV68_14900"
     CDS             complement(1561..2352)
                     /locus_tag="PLV68_14900"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRE02587.1"
                     /translation="MSVSSPAAARIAARNWAFFRHVWKANLFNSLLQPVLYLLAMGLG
                     VGALINDNSSSTSMLHGLSYAAFVGPGLLATTTMMIASVESMWPVYDGVAWGRHYESV
                     VATPLTPGDIAWSHALWMAVRSGLGAITVALAMALFPAVRSLGLVPATAVATLGGVAI
                     GMPLAAWAISRQREGSFPIIQRFVIVPLFLFGGAFFPIEQLPLVLRWVGYASPLWHNV
                     ELCRALVSATPITFAGALGHIAYLAGWALAGGVVMARCARRRLYP"
     gene            complement(2369..>2934)
                     /locus_tag="PLV68_14905"
     CDS             complement(2369..>2934)
                     /locus_tag="PLV68_14905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRE02588.1"
                     /translation="EFAQLSERADSRVEPLSGGMKRRITIARALINNPELLLLDEPTT
                     GLDPQARHILWDRLYRLKQQGVTLVLTTHYMDEAEQLCDRLVVMDGGRIVAEGSPRQL
                     IERHATREVLELRFSAGTNADNVHYAEGIGDRWEVLPDRILVYAHDGDAALAEVHARG
                     GRPESALVRRSTLEDVFLHLTGRSLIE"
BASE COUNT          573 a          969 c          965 g          427 t
ORIGIN      
        1 caacaacgcg gagcaccaca gaaaattccc aagaactaac gactcacgac actagaggag
       61 cagggtgccg cggacgatgt cgaagatttc gttggggtgg tcgacgatcc agcgcgcgcc
      121 ggctctttcc aactcgtcgt cgctggcggc gtaaccccag cggacaccga cgcagtcgat
      181 gccgaactgg gccgagcctt cgacgtcgtg atatcggtcg ccgatcatca cggggcggca
      241 ctgtccgagc tcttcgagca cgtaggccaa tacggctgcc ttggtggagc gggtcatgtc
      301 gaagctggcc ccgccgatga cgtcgaagcg accggtgtag ccccaatgct ccaagatgcg
      361 ccgggcggtc ggttcaggct tggacgtacc gatcgcgata cggaacccgt ctgcggccaa
      421 gccgtcgagc agcgcctcca tgccgggata gacgaaattc tcgaacacgc cgatgtccca
      481 gtagtgctcg cggtagaccg agatcaacgt cttgacctcg tcgggtgaga agccctcggc
      541 tcggaaggcc gtctcgaacg gcggaccgat gaagcggcgc aggttgggcg ggggcgggta
      601 gccggtctgg gtgaaggcgt ggtcgagcga tgcgaggatg ccgggcgcgg agtcagagat
      661 cgtgccgtcg agatcgaaga tcacggtgtc gaacggagca tctgggcgag gcatggtggc
      721 gtgttcgttg gtggcgtggt gaaaggagaa cgttgggttg ctacttgagc aacaggacct
      781 gcaagcggcg ggacaccacc cacagaccgg cgacgcacat cgtaaggaga aacgcgacgt
      841 gtcctgcaat cgcccacgtc gcctcgccag tgttggccgc gcgcacgagc gcgacgccgt
      901 gatacagcgg cgagagctgc accacccaac cccagtcgcc gtacgagctg agcggataga
      961 aggtggccga gaacaagaac agcggcatcg tcgcggtctg cacccactcg aagtcgctcc
     1021 acgagcgcat gtaggtggtg gcggccatgc cgaccgacgc gaaggcgaag ccgatcagca
     1081 cggcggcagg caacgacaac accatccatc ctgagcgcac caggccgaac gggatcatcg
     1141 tcaccaagaa cgcggccgag tacagaaagc cgcggatcaa cgcccacagg atctcgccga
     1201 gggcgacgtc accgaccgtc atcggcgtgg ccaggatcgc gtcgtaggtc ttcgcgtaac
     1261 gcagcttgtg gaacacgttc atcgtcgagt cgtacaccgc cccgttcatc gcgctggcgg
     1321 ccagcaacgc gggagcgacg aactcggcgt agccgatcgt gcgcccaccg acggtgacgt
     1381 cgccgaccaa cttcgacacc ccgatgcgga tcgacaacag gtagaacagc ggttcgaaga
     1441 acccggagat caggatcggc cacgtgttgc ggtagcacat catcgtccgc tcgaccatcc
     1501 gctgcggacg ccgcgagttg accaccaccg gcggcatgat ccgcaacacc aagccgccgt
     1561 tcatgggtac aaccgccttc gggcacagcg ggccatgacg acgccgcctg cgagcgccca
     1621 cccggcgagg taggcgatgt ggcccagcgc tccagcgaag gtgatcggcg ttgccgacac
     1681 cagggcacgg cacaactcca cgttgtgcca cagcgggctc gcgtagccca cccagcgcag
     1741 caccagcggt agctgctcga tgggaaagaa cgcgcctccg aacaggaaca gcggcacgat
     1801 cacgaagcgc tggatgatcg gaaagctgcc ttcgcgctgg cggctgatcg cccacgcggc
     1861 cagcggcatg ccgatcgcga caccacccag cgtcgccacc gccgtcgcag ggaccagccc
     1921 caacgagcgc acggccggaa acagtgccat cgccagcgcg acggtgatcg cgccgagacc
     1981 cgaacggacc gccatccaca gcgcgtgcga ccaggcgatg tcgccggggg tgagcggcgt
     2041 cgcgacgacc gactcgtagt gacgacccca ggccacgccg tcgtacaccg gccacatgct
     2101 ctcgaccgag gcgatcatca tcgtggtcgt cgccagcaag cccggcccga cgaaggccgc
     2161 gtagctgagc ccgtgcagca tcgaggtgct cgagctgttg tcgttgatga gcgcgccgac
     2221 gccaaggccc attgccagca ggtacagcac cggttgtagc agcgagttga acaggttggc
     2281 cttccacaca tggcggaaga acgcccagtt gcgcgctgcg atgcgcgcgg cagccggtga
     2341 actcacgctc atccgaactc gctgccgctc attcgatcag actccggccg gtgaggtgga
     2401 ggaacacgtc ctccaacgtc gagcggcgca cgagggcgct ttcgggtcgg ccaccgcggg
     2461 cgtggacttc ggccaaggcc gcgtcgccgt cgtgggcgta gaccaggatc cggtcgggca
     2521 gtacctccca ccgatcgccg atgccctcgg cgtagtgcac gttgtcggcg ttggttcccg
     2581 cagagaagcg caactccaac acctcgcgcg ttgcatgccg ctcgatgagt tgacgcggtg
     2641 aaccctcggc cacgatccgc ccgccgtcca tcaccaccag ccggtcgcag agctgttcgg
     2701 cctcgtccat gtagtgggtc gtcagcacca gggtgacgcc ctgttgcttc agccggtaga
     2761 gccggtccca caggatgtgg cgggcctgcg ggtcgagccc cgtggtcggc tcgtcgagca
     2821 gcaacaactc ggggttgttg atcaacgcgc gcgcaatcgt gatgcgccgc ttcatcccac
     2881 cgctcaacgg ttcgacccgg ctgtcggcgc gctcgctgag ctgcgcgaac tcca
//
LOCUS       DAORNF010000415         2928 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_25618_length_2928_cov_1.550644, whole genome shotgun sequence.
ACCESSION   DAORNF010000415 DAORNF010000000
VERSION     DAORNF010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2928
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25618_length_2928_cov_1.550644"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>929
                     /locus_tag="PLV68_14910"
     CDS             <1..>929
                     /locus_tag="PLV68_14910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HRE02589.1"
                     /translation="KGGSGKSVIASNLAVLLAQRSDRPVVLVDADLQVRARATREFPQ
                     HFPQPGWVEHDPVEIWQAARDTLAEVIAQLDAPPAAIGITNQRETIVAWSRATGLPLH
                     RAIVWQDRRTATHCEQLAADGMLPLVRSRTGLVLDPYFSATKCAWLLRDGGVAIDDDL
                     ALGTIDTWLLWNLTGGDVFATDPSNASRTMLFDITSLAWDAELCDVFGVPMHALAEVR
                     PSSGDFGVTNPRCGVAAGIPICGIAGDQQAALFGQLCVHPGMAKNTYGTGSFALLNVG
                     PTCPAPAEGMLTTVGWTMADGTTAYAVEGAIFL"
     assembly_gap    930..1007
                     /estimated_length=78
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1008..1497
                     /locus_tag="PLV68_14915"
     CDS             <1008..1497
                     /locus_tag="PLV68_14915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HRE02590.1"
                     /translation="SVADNGGVFVVPAFTGLGSPWWDPHARGSIIGITRGTTRGHIAR
                     AVVESIALQVTDAVTAMTGAAGTPLREMRVDGGASAMDSMLQLQADLLGVTVQRPVDH
                     ETTALGAAYLAGLAAGVWPSLDALATQWQLDVEFRPSDDRAAAQQLHTAWLRAVQRSR
                     SA"
     assembly_gap    1551..1714
                     /estimated_length=164
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1715..2154)
                     /locus_tag="PLV68_14920"
     CDS             complement(<1715..2154)
                     /locus_tag="PLV68_14920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02591.1"
                     /translation="MSSYVVRVWLPDRPGALGLVASRIGAVHGDVVGIEILERGGGKA
                     IDELVVALPDGVSRELLLAEISQVDGVAIEDVRPIHPDRPDAGMLALQISARLTEAEP
                     ANLLGVFCDELVGLVDGAWASALRVDGTAVVTRGDPPDLAWLAA"
     gene            complement(2217..>2606)
                     /locus_tag="PLV68_14925"
     CDS             complement(2217..>2606)
                     /locus_tag="PLV68_14925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRE02592.1"
                     /translation="KSYLDYGTFQPVQIAATVTMNEAPDFPAEVTAVYKGRRDALIDG
                     LARIGWEIPKPKGSMFVWAPIPEPYADMDSVEFCSHIVRECDVALSPGAGFGPGGEGF
                     VRFALIENEQRIAQGIRNLRRGLTKLG"
     assembly_gap    2607..2690
                     /estimated_length=84
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2691..>2928)
                     /locus_tag="PLV68_14930"
     CDS             complement(<2691..>2928)
                     /locus_tag="PLV68_14930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRE02593.1"
                     /translation="YEVGWPKPRVLVISFPHNPTGAVVDLDFMQRVVDFCREREMIVV
                     HDFAYADMGFDGHKPPSILQADGAKEGAVELYSMT"
BASE COUNT          406 a          942 c          861 g          393 t
ORIGIN      
        1 caagggcggc tcgggcaagt cggtgatcgc ctcgaacctg gcggtcctgc tggcccagcg
       61 cagcgatcgc cccgtcgtgc tcgtcgacgc cgacctccag gtccgcgccc gcgccacccg
      121 cgagttcccg cagcactttc cgcagcccgg ctgggtcgag cacgacccgg tcgagatctg
      181 gcaagcagct cgcgacaccc tcgccgaggt catcgcccaa ctcgacgcgc cgcccgccgc
      241 gatcggcatc accaaccaac gcgagacgat cgtcgcctgg agccgcgcca ccggcctgcc
      301 gctgcaccgc gcgatcgtgt ggcaggaccg ccgtacggct acccactgcg agcagctcgc
      361 cgccgatggc atgctcccgc tggtgcgcag ccgcacgggg ctggtgctcg acccgtactt
      421 cagcgcgacc aagtgcgcgt ggctgctccg tgacggcggc gtcgccatcg acgacgacct
      481 ggcgctgggc accatcgaca cgtggctgtt gtggaacctc accgggggcg acgtgttcgc
      541 caccgatccg tccaacgcca gccgcacgat gctgttcgac atcacatcgc tggcgtggga
      601 tgccgaactg tgcgacgtgt tcggtgtgcc gatgcacgcg ctggctgagg tgcgcccgtc
      661 aagcggcgac ttcggtgtca ccaacccacg ctgcggcgtg gcggccggca tcccgatctg
      721 cggcatcgcc ggcgatcagc aagcggccct gttcggtcag ctctgcgtcc accccggcat
      781 ggccaagaac acctacggca cgggcagctt cgcgctgttg aacgtcggtc ccacctgtcc
      841 tgcaccggcc gagggcatgc tcaccaccgt cggatggacc atggccgacg gcaccaccgc
      901 ctacgccgtc gagggcgcca tcttcctcan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngtc ggtggccgac
     1021 aacggcggtg tgttcgtcgt gcctgccttc accggtctgg gcagcccgtg gtgggacccg
     1081 cacgcgcggg gatcgatcat cggcatcacc cgcggcacca cccgtggcca catcgcccgg
     1141 gcggtggtcg agtcgatcgc actgcaggtc accgacgcgg tcaccgcgat gaccggcgcg
     1201 gcgggtacac cgctgcgcga gatgcgcgtc gacggtggcg catcggcgat ggactcgatg
     1261 ctgcaactcc aggccgacct gctcggcgtc accgtgcaac gcccggtcga tcacgagacc
     1321 actgcactcg gcgccgccta cctggccggt ctggccgcag gggtctggcc atcgctcgac
     1381 gccctcgcca cccagtggca actcgacgtc gagttccgcc ccagcgacga tcgggccgcc
     1441 gcccagcagt tgcacaccgc gtggctgcgc gccgtccaac gctcccgctc ggcttgatcc
     1501 attcggcctg atctggtcag gcccggtcga acgccgcggc caggcgggcg nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnaatgca gcgagccacg ccaggtcggg
     1741 cgggtcgccc cgcgtgacca cggcggtgcc gtcgacgcgc agggcgcttg cccatgcgcc
     1801 gtcgaccaga ccgaccagct cgtcgcagaa cacgccgagc aggttggccg gctcggcttc
     1861 ggtcagccgg gccgagatct gcaaggcgag catgcccgcg tcgggacgat cggggtggat
     1921 ggggcgcaca tcctcgatcg ccaccccgtc gacctgggaa atctcggcca acagcagttc
     1981 gcggctcacg ccgtcgggca gggcgacgac cagttcgtcg atcgccttac ccccgccgcg
     2041 ttcgaggatc tcgatgccga ccacgtcgcc gtgcacggca ccgatccgac tggctaccag
     2101 tcccaacgct cccggtcgat cgggaagcca gacacgcacg acgtatgagc tcacgcgccg
     2161 caagatagcg atcgaaccga cacgcgagtt gtcagatttg tgtcggccgg atgaactcag
     2221 ccgagtttgg tgaggccgcg gcgcaggttg cggatgcctt gcgcgatgcg ctgctcgttc
     2281 tcgatcagcg cgaagcggac gaacccctca ccgccggggc caaagccggc accgggcgac
     2341 aaggcgacgt cgcactcgcg cacgatgtgc gagcagaact cgaccgagtc catgtcggcg
     2401 tacggctcgg ggatcggtgc ccacacgaac atcgaaccct tcggcttggg gatctcccag
     2461 ccgatgcggg ccagcccgtc gatcagcgcg tcgcgacggc ccttgtacac ggcggtgacc
     2521 tcggccggga agtcgggcgc ttcgttcatg gtgaccgtgg ctgcaatctg gaccggctgg
     2581 aacgtgccgt agtcgaggta gctcttnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ggtcatgctg
     2701 tacagctcga ccgcaccctc cttggcaccg tcggcctgca ggatcgacgg cggcttgtgg
     2761 ccgtcgaagc ccatgtcggc gtaggcgaag tcgtggacca cgatcatctc ccgctcgcga
     2821 cagaagtcga ccacgcgctg catgaagtcg aggtcgacca ccgcaccggt ggggttgtgg
     2881 gggaagctga tcaccagcac ccgcggcttg ggccagccaa cctcgtag
//
LOCUS       DAORNF010000416         2927 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_25637_length_2927_cov_1.412953, whole genome shotgun sequence.
ACCESSION   DAORNF010000416 DAORNF010000000
VERSION     DAORNF010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2927
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25637_length_2927_cov_1.412953"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>263
                     /locus_tag="PLV68_14935"
     CDS             <1..>263
                     /locus_tag="PLV68_14935"
                     /inference="COORDINATES: protein motif:HMM:NF014860.2"
                     /note="catalyzes the reduction of mycothione or
                     glutathione to mycothione or glutathione disulfide;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="mycothione reductase"
                     /protein_id="HRE02594.1"
                     /translation="SHNIAHPDDLRAADLWPTPHAVFAGPQVASVGLTEQELRRAGRP
                     YVAASQRYGDVAYGWAMEDTDHFCKVIADPDTRLLLGAHIIGP"
     assembly_gap    264..472
                     /estimated_length=209
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            505..>689
                     /gene="thrH"
                     /locus_tag="PLV68_14940"
     CDS             505..>689
                     /gene="thrH"
                     /locus_tag="PLV68_14940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442456.1"
                     /note="catalyzes the formation of serine from
                     phosphoserine; also has phosphoserine:homoserine
                     phosphotransferase activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoserine
                     phosphatase/homoserine phosphotransferase ThrH"
                     /protein_id="HRE02595.1"
                     /translation="MTLDLEGVLVPEIWIAVAERTGIDALRRTTRDEPDYDVLMRGRL
                     DLLDHHGLSMSTIADVIG"
     assembly_gap    690..801
                     /estimated_length=112
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <802..1084
                     /gene="thrH"
                     /locus_tag="PLV68_14945"
     CDS             <802..1084
                     /gene="thrH"
                     /locus_tag="PLV68_14945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442456.1"
                     /note="catalyzes the formation of serine from
                     phosphoserine; also has phosphoserine:homoserine
                     phosphotransferase activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional phosphoserine
                     phosphatase/homoserine phosphotransferase ThrH"
                     /protein_id="HRE02596.1"
                     /translation="TLICHRLVVEHDRIVDYRLRMPDQKRHAVAALHGLRYRVVAAGD
                     SYNDTAMLAEADAGFLFHAPANVIAEFPQFPALDSYDELLTHITAALAD"
     gene            complement(1097..1630)
                     /locus_tag="PLV68_14950"
     CDS             complement(1097..1630)
                     /locus_tag="PLV68_14950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HRE02597.1"
                     /translation="MTTPPTTPPTTTPTTPPTTTAGTQGTTHAATPVAHYLAPGWFTR
                     RVFNPVIGWLTRRGLSVRGSAVLEVVGRRSGVVRTAVVNPLSVDGAVYLVAPRGNTEW
                     VRNVRAAGAATLLQRRRRTTYTVVEVGTAEAVPVLRHYLVKWSAETGTFFDGLTASST
                     DHEIAAAAQRHPVLRLN"
     gene            1791..2450
                     /locus_tag="PLV68_14955"
     CDS             1791..2450
                     /locus_tag="PLV68_14955"
                     /inference="COORDINATES: protein motif:HMM:NF024701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRE02598.1"
                     /translation="MTAIKTEARRQLAEVGAPALSLRSIARELGMVSSAVYRYVSSRD
                     ELLTLLIVDAYDSIGAAAEHAAGSSRTRSPAKRWLTVCRAVRTWALAHPHEYALVYGS
                     PVPGYAAPVDTISPAIRVSAALIGVIADAHAAGHVGRGGELGRIPRQVRRDADAIAAA
                     LGTDLPDEVVIRALIAWTQLFGLISFELFGQTRNGITDHDALFTVTTTATATLLGLTT
                     E"
     gene            complement(2465..2644)
                     /locus_tag="PLV68_14960"
     CDS             complement(2465..2644)
                     /locus_tag="PLV68_14960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02599.1"
                     /translation="MSPTLLILPRRQPRAPEPFTPEQVSAIDPQVGLGEFAGRLSGSE
                     MQAVDAALRAVLSLD"
     gene            2643..>2927
                     /locus_tag="PLV68_14965"
     CDS             2643..>2927
                     /locus_tag="PLV68_14965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family peptidase"
                     /protein_id="HRE02600.1"
                     /translation="MADLGLMLGDLRALVEVESPSRDLVALAASAAAVSDVIEARLGS
                     RPTLIDTAAGPIVHWSGCGAPRVVVIGHHDTVHPLGTLAARPFAVRDGIAT"
BASE COUNT          387 a          920 c          885 g          414 t
ORIGIN      
        1 cagccacaac atcgctcacc ccgacgacct tcgcgcggcc gacctatggc ccaccccgca
       61 tgcggtgttc gccggtccgc aggtcgcctc ggtcggcctg accgagcagg aattgcgccg
      121 cgccgggcgg ccgtacgtgg ccgcttcgca gcgctacggc gatgtcgcct acggctgggc
      181 gatggaggac accgaccact tctgcaaggt catcgccgac cccgacaccc ggctgttgct
      241 gggggcgcac atcatcggtc cgcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnccgtgact
      481 gcgagcgaga cccgccagac catcgtgact ctcgatctgg aaggcgtgct ggtccccgag
      541 atctggatcg ctgtggccga gcgcaccggc atcgacgcgc tgcggcgcac gactcgcgac
      601 gaacccgact acgacgtgtt aatgcgcggt cggctcgacc tgctcgacca ccacgggctg
      661 tcgatgtcga cgatcgccga cgtgatcggn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn ngacgttgat ctgccatcgc ttggtggtcg agcacgatcg
      841 catcgtcgac tatcggttgc ggatgcccga ccagaagcgt cacgcggtcg ccgcgctgca
      901 cggtctgcgc tatcgcgtcg tggccgcggg cgactcgtac aacgacacgg cgatgctggc
      961 cgaagccgac gccgggtttc tgttccacgc cccggccaac gtgatcgccg agttccctca
     1021 gttccccgcg ctcgacagct acgacgaact gctcacccac atcaccgccg cgctcgccga
     1081 ctgagcaccc ggccgctcag ttcaggcgca gcaccgggtg tcgttgggcc gcggcggcga
     1141 tctcgtggtc ggtcgatgac gcggtgaggc cgtcgaagaa ggtgccggtc tcggccgacc
     1201 acttgaccag gtagtgccgc aggacgggca cagcttcggc ggtgccgacc tcgacgacgg
     1261 tgtaggtcgt gcgccggcgg cgttgcagca gggttgcggc acccgcggcg cggacgttgc
     1321 ggacccactc ggtgttgccg cggggagcga cgaggtagac cgcgccgtct actgacagcg
     1381 ggttgaccac cgccgtacgg acgacacccg accgccgtcc caccacctcc aacacagccg
     1441 agccgcgcac ggacagcccg cgccgggtca gccatccgat gacggggttg aagacccgcc
     1501 gggtgaacca gccgggagcc agatagtgcg cgaccggcgt tgccgcgtgc gtcgtgccct
     1561 gtgtgcctgc cgtcgtcgtc ggtggtgtcg tcggtgtcgt cgtcggtggt gtcgtcggtg
     1621 gtgtcgtcat gtctcatctc tcctcgttgt gagcgctgct ctcgaaaagg agtcaaccag
     1681 acggagggct acgttgcaag agcagtgctc acatttggga caacaggtct ggcagactgg
     1741 cgctcatgcc accgtcgacc gggatccgtg aacgtgtcag aacggagacg atgaccgcca
     1801 tcaagaccga ggcccggcgg cagttggccg aggtcggcgc accggcgctg tcgttgcgca
     1861 gcatcgcccg cgagctcggg atggtgtcgt cggcggtgta ccgctacgtg tccagccgcg
     1921 acgagctgct gacgttgttg atcgtcgacg cgtacgactc gatcggcgcc gccgccgagc
     1981 acgctgccgg ctcgtcacgc acccggtcac ccgccaagcg ctggctcaca gtgtgtcgcg
     2041 ccgtgcggac gtgggcgctc gcccaccccc atgaatacgc cctcgtttac ggctcgccgg
     2101 tacccggcta cgcggccccg gtcgacacga tctcacccgc gatccgggtc agcgccgcgc
     2161 tcatcggcgt gatcgctgac gctcacgctg cgggccacgt cgggcgcggc ggcgaactcg
     2221 ggcgcatccc gcgccaggta cgccgcgatg ctgacgccat cgcggccgcg ctcggcaccg
     2281 acctccccga cgaggtggtg atccgcgccc tgatcgcctg gacgcaactg ttcggcctga
     2341 tcagcttcga actgttcggc cagacccgca acggcatcac cgaccacgac gccctgttca
     2401 ccgtgacaac caccgcaacc gccaccctcc tcggcctcac aaccgagtga tgaacccggc
     2461 gcgttcagtc gagtgacagc acggcacgca gcgcagcgtc gaccgcctgc atctcgctgc
     2521 cgctcaaccg acctgcgaac tcgccgagcc ccacctgggg atcgatcgcc gagacctgct
     2581 cgggcgtgaa aggctcgggt gctcggggtt gtcggcgggg cagaatgagg agcgtgggtg
     2641 acatggctga tctggggttg atgctcggcg atctgcgcgc gttggtcgag gtggagtcgc
     2701 cgtcgcgtga cctcgttgcg ctcgccgcgt cggcggctgc ggtgagcgac gtcatcgagg
     2761 cgcggctcgg gtcgcggccc acgctaatcg acacggccgc ggggccgatc gtgcactgga
     2821 gcggctgtgg cgcgccgcgg gtggtcgtga tcggccacca cgacacggtg cacccgctgg
     2881 gcacgctggc cgcgcggccg tttgccgtcc gcgacggcat cgccacc
//
LOCUS       DAORNF010000417         2918 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_25760_length_2918_cov_1.561299, whole genome shotgun sequence.
ACCESSION   DAORNF010000417 DAORNF010000000
VERSION     DAORNF010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25760_length_2918_cov_1.561299"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    326..384
                     /estimated_length=59
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <385..>930
                     /locus_tag="PLV68_14970"
     CDS             <385..>930
                     /locus_tag="PLV68_14970"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRE02601.1"
                     /translation="DDSIAQHVADSITSAGGSAIANHDSVGSFAGAESIVTAAIDTFG
                     RLDQLVLCAGNSAQVPPQEMTEEFWDLTMAIHLKGHFACMRAAMDPMSASGGGAIVAI
                     TSHVGLYGLPDAPAYCAAKSGITGLTKSMAQAYQPLGITVNCVAPSAVTRMSDTVPVD
                     VLRQRAAAAGVTLPDDMSDDA"
     assembly_gap    931..949
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <950..1177
                     /locus_tag="PLV68_14975"
     CDS             <950..1177
                     /locus_tag="PLV68_14975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02602.1"
                     /translation="DPVAVANFIAYLGTEAAAQVTGQIFAVIGGHVGVFAPWTEGNSA
                     DSVVPWTIDQLTEQVPAMLDGAEATAPGGRT"
     assembly_gap    1351..1374
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1375..2286
                     /locus_tag="PLV68_14980"
     CDS             <1375..2286
                     /locus_tag="PLV68_14980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02603.1"
                     /translation="ATIFMAIPHTDDTPSMSDNAFIRTGLHLPQYRDGRRFDIEEADG
                     TVTWRVDDREYVWDPPNWRIRGEHAGVDVDLVAQAVGPGTWAAGPFETAAENKAFGYD
                     VPIGVTGTITADGRTFQLDDAHGSHERAGMGQGRDIVAEVDGAEVLVADLFHDDIHMI
                     FGQHTGRSLNFGRIDAGSNSVSIMPLMDQGVMSVTTLERWHDPKSGLLLPVHWRVVMS
                     NEGANCELDVRAEGRAYWHYTTKSGVMVMVWQLSRANGTLYLPDGTQREIKDALTASR
                     WGRSLLVADETTDGPRFAVLDELAAKQ"
     assembly_gap    2495..2534
                     /estimated_length=40
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2535..>2918
                     /locus_tag="PLV68_14985"
     CDS             <2535..>2918
                     /locus_tag="PLV68_14985"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRE02604.1"
                     /translation="SENFIVPLGTEQDSTYVYLTNEPLVYLQHETLEFVPGLAQSWDV
                     SADGLSWTFNLRSGVTFHNSDGSAAGSFTSDDVKFTIETAMGANSTWYALSTWQALLD
                     AIETPDPQTVVIKLKAPYPGLIGDVS"
BASE COUNT          551 a          910 c          861 g          454 t
ORIGIN      
        1 cagcggtcgt caagacgtca tcggaccgca acgacagcgg cgttcaccgc aatccaacga
       61 tccttcgact cttgcttgac aaccttcatg agccgcgcta attctaacag tcggttggta
      121 gttggccttc tgctcacttc agggtcgtac caccatcatc cacaccgggg aatacgggaa
      181 acactagacg ggcgcttcac gcgtcgctgt cggcgatccc ttgggagaaa catgacaaac
      241 agcggcaagc tcagcggcaa ggtcgccatc gtgaccggtg gcggaggcgg aattggccgg
      301 tccgccgcca cggtcctcgc ctcacnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnngtgacg acagcatcgc tcaacacgtc gccgactcga
      421 tcacatcggc cggcggcagc gctatcgcca accacgactc cgttgggtcc ttcgccgggg
      481 ccgagagcat cgttacggcg gcgatcgaca cgttcgggcg acttgaccaa ctcgtgctct
      541 gcgcaggcaa ctcggcacag gtgcctccac aggagatgac tgaagagttc tgggacctga
      601 cgatggcgat ccacctcaag ggtcacttcg cctgcatgcg cgcagccatg gacccgatga
      661 gcgcaagcgg tggcggagcg atcgtggcga tcacctccca cgtcggactg tacgggctcc
      721 ccgacgcccc ggcatattgc gctgccaagt ccggcatcac cgggctgacg aagtcgatgg
      781 cacaggccta ccagccgctg gggatcacgg tcaactgcgt cgctccgagt gccgtgaccc
      841 gcatgtccga caccgtcccg gtagacgtgc tgcgccaacg tgcggccgcc gctggcgtca
      901 cactgcccga tgacatgtcc gacgatgcga nnnnnnnnnn nnnnnnnnng atccggtggc
      961 cgttgccaat ttcatcgcct atctcggcac cgaagccgcg gcgcaggtca ccggtcaaat
     1021 ttttgccgtg atcggcggcc acgtcggcgt cttcgcaccg tggacagaag gcaactcggc
     1081 cgacagcgtc gtcccatgga cgatcgacca gctcacggaa caggttccgg cgatgctcga
     1141 tggggccgag gcgaccgcac caggaggcag gacatgaacg acatccccca gctatcgcat
     1201 ggctatcagc aggttgacac ggtgctcccg gccgatgagg actcatcgga gaacgcggga
     1261 ttcgtcgtgt tcggcacgag cggcgaccac gacgtgctgt tctggtcatg tgcatacatg
     1321 atgggatcag cgccgcacac gcagtggctc nnnnnnnnnn nnnnnnnnnn nnnngccact
     1381 atcttcatgg cgatcccgca caccgacgac acaccgtcga tgtcggacaa tgcgttcatt
     1441 cgcacgggtc tgcacctgcc gcaataccgg gacgggcgcc gcttcgacat cgaagaggcc
     1501 gacggaacgg tgacatggcg cgtcgacgac cgcgagtacg tatgggaccc acccaactgg
     1561 cgcatccgtg gcgagcatgc gggcgtcgat gtcgatctcg tcgcccaggc ggtgggacct
     1621 ggcacatggg ccgctggacc cttcgagacc gcggccgaga acaaggcgtt cggctacgac
     1681 gtgcccattg gtgtcaccgg caccatcacg gcagatgggc gcacgtttca actcgacgac
     1741 gcgcacggct cacacgagcg tgcaggcatg ggtcaaggcc gcgacatcgt tgccgaggtc
     1801 gacggcgccg aggtactcgt cgctgatctg ttccacgacg acatccacat gatctttggc
     1861 cagcacacag gtcgcagcct gaacttcggc cgcatcgatg ctggcagtaa ctcggtctcg
     1921 atcatgccgc tgatggatca aggggtgatg tcggtcacca cgctcgagcg ctggcacgat
     1981 ccgaagagcg gcctcctgct acctgtgcac tggcgcgtcg tgatgtcgaa cgagggtgcc
     2041 aactgcgagc tagacgtacg cgccgagggc cgagcttact ggcactacac gacgaagtcc
     2101 ggcgtgatgg tgatggtgtg gcagctctcc cgcgccaacg gaacgctcta cttgcccgat
     2161 ggaacacagc gggaaatcaa ggacgcactc acggccagtc gttggggtcg ctcgctgctc
     2221 gtcgccgacg agacgacgga tggcccacgg ttcgcagtgc tcgacgagtt ggctgccaag
     2281 caatgacaca gaaccacgca ctgaacaagg ggagttcacc aatgagtagg aacgcacgaa
     2341 acagggcccg ccatcaaggc gcgggaaccg cactgctgct ggcgagcgcg ctgttgatcg
     2401 cagcatgcgg gagcgacgag aagtcgtcgc cgacgaccag tggagcgacc cagacgaccg
     2461 cggggtcgac gactctgcca gcaccaacgg gaccnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnntccgag aacttcatcg tgccgctcgg caccgagcag gacagcacct
     2581 acgtgtacct gaccaacgag ccactcgtct atctgcagca cgagacgttg gagttcgtcc
     2641 cagggctcgc gcagagctgg gacgtctccg ccgacggcct cagttggacg ttcaaccttc
     2701 gcagcggggt cacgttccac aacagcgatg gctccgcagc gggttccttc acgtccgatg
     2761 acgtgaagtt caccatcgag accgccatgg gggccaacag cacctggtac gccctgagca
     2821 cctggcaggc cctgctcgac gccatcgaga cgcccgaccc acagaccgtc gtcatcaagc
     2881 tcaaggcgcc gtacccgggt ctgatcggcg acgtctcg
//
LOCUS       DAORNF010000418         2911 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_25820_length_2911_cov_1.776261, whole genome shotgun sequence.
ACCESSION   DAORNF010000418 DAORNF010000000
VERSION     DAORNF010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2911
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25820_length_2911_cov_1.776261"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1117)
                     /gene="uvrC"
                     /locus_tag="PLV68_14990"
     CDS             complement(<1..1117)
                     /gene="uvrC"
                     /locus_tag="PLV68_14990"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HRE02605.1"
                     /translation="MVQRPPTGTIPDAPGSYQFKDAAGRVIYVGKAASLRQRLGHYFQ
                     DARHLHGRTAQMVATAESVEWIEVRNEVEALMLEWTLIKQHRPRFNIRLRDDKSYPFL
                     AVTLDEEYPRAMVMRGRKRKGVRYFGPYAHAYAIRDTLDLLLRSFPVRTCSNSKFNEH
                     HRIGRPCLLYHIEKCSGPCVAAIERDEYLTQVEELCSFLDGDTDEIVTRLERDMRTAA
                     GELEFERAARLRDRLTAVQKAIAKQQMVAERSEDIDVIGIADDELEAAIQVFYVRKGR
                     VVGRKGFIVDKVEDLTPGGLVDRIMEEMYGDEPPLGIPKQVLVPVDAQDQATYEEWLS
                     HMRGSRVQIRVPQRGDKRELHETVTRNAKDEFTRHRLR"
     gene            1254..2141
                     /locus_tag="PLV68_14995"
     CDS             1254..2141
                     /locus_tag="PLV68_14995"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HRE02606.1"
                     /translation="MPGRYRFSMPSHRDRNDPWFRIGLIDVTTTLLIAALCVASFFVY
                     AADQTLLFHLFLIPSENRLTGSVLGGQVWRLVTWPIANEPSIWTAIAIAIFWYFGSQL
                     EAALGRVRYLWLIALTVLIPGLVAVAVDTQVWGIRTLEIAIFVLFVLDNPRMPFFFGL
                     PAWILAVVIVGIDILQLLGNRQTGYLLVYLVALATAALVARCFGMLADYPWLPQIVDR
                     SGSRRRVRQRTGRADASHQTVVSGPWPSAPPTYTPMQDQAEVDRILDKIAAVGMDGLT
                     ADEKRRLNEASKRLRKGTN"
     gene            2220..2816
                     /locus_tag="PLV68_15000"
     CDS             2220..2816
                     /locus_tag="PLV68_15000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02607.1"
                     /translation="MFGLATACTSGASEPVGRPDAEPLPISVLYRTERQYFHTIEDIS
                     AASDVVIIATVVSVDPADVPRPADEPDGSGTEFVRLSLRADTVLKGDVPSELGVSWEA
                     YVVDNGARTLEIAIDGVVAPRTSDQVLLFLKWADPSQGALGITPESPWYIVTMDGIYW
                     IDGDTVTSPITDPKRLASTVNGQSLADIVAAISATPSG"
BASE COUNT          435 a          990 c          947 g          539 t
ORIGIN      
        1 ggcgcagccg gtggcgggtg aactcgtcct tcgcgttgcg ggtcaccgtc tcgtgcagct
       61 cgcgcttgtc gccacgttgg ggcaccctga tctgcacccg tgacccgcgc atgtggctga
      121 gccactcctc gtacgtcgcc tggtcctggg cgtcgacggg taccagcacc tgcttgggga
      181 tgcccaacgg cggctcgtcg ccgtacatct cctccatgat ccgatcgacc aggccgcccg
      241 gggtcaggtc ctccaccttg tcgacgatga agcccttgcg cccgaccacc cggcctttgc
      301 gcacgtagaa cacctggatc gccgcttcga gctcgtcatc ggcgatgccg atcacgtcga
      361 tgtcttcgct gcgctcggcc accatctgct gcttggcgat cgccttctgc accgcggtca
      421 accggtcgcg caggcgcgcc gcgcgctcga actccaactc accggccgcc gtgcgcatgt
      481 cgcgctccaa gcgggtgacg atctcgtcgg tgtcaccgtc gaggaacgag cacagttcct
      541 cgacctgggt caggtactcg tcgcgctcga tcgcggcgac gcacgggcca ctgcacttct
      601 cgatgtgata cagcaggcac ggccgcccga tgcggtggtg ctcgttgaac ttcgagttgc
      661 tgcacgtccg caccgggaag ctgcgcagca gcagatcgag cgtgtcgcgg atggcgtagg
      721 cgtgggcgta ggggccgaag tagcgcacgc ccttgcgctt gcgaccgcgc atcaccattg
      781 cccgcgggta ctcctcgtcc agcgtcacgg ccaggaaggg gtagctcttg tcgtcgcgca
      841 ggcggatgtt gaaccgcggc cggtgctgct tgatcagcgt ccactccagc atcaaggcct
      901 cgacctcgtt gcggacctcg atccactcga ccgactcggc ggtcgcgacc atctgcgcgg
      961 tgcgcccgtg caggtgacgg gcgtcttgga aatagtgccc gagccgctgg cgcaggctgg
     1021 ccgccttgcc cacgtagatc acccggccgg ccgcatcctt gaactggtac gagccgggtg
     1081 cgtcggggat cgtgccggtc ggcggtcgct gcaccataac gacgttgagc ctacggagcg
     1141 acgtcgaacc tggggcgacg ctgcgctctg gcgggcttgg gcggcagcgc tacccaggcc
     1201 ggctggcggc tctgcccagg ctcatcgcgg tgccacgggt aggatcggcg ccgatgccag
     1261 gccgctaccg gttctccatg ccctctcatc gcgaccgcaa cgatccgtgg ttccgcatcg
     1321 ggctgatcga cgtcacgacg acgttgctga tcgcggcgct gtgcgtcgcc tcgttcttcg
     1381 tctacgccgc cgaccagacg ctgctgttcc acctgttctt gataccgtcg gagaatcggt
     1441 tgaccggatc ggtcctcggc ggccaggtgt ggcgcctggt gacgtggccg atcgccaacg
     1501 agccgtcgat ctggaccgcg atcgcgatcg cgattttctg gtacttcggc agccaactgg
     1561 aagccgctct gggccgggtg cggtacctgt ggctgatcgc cctgaccgtg ctgatccccg
     1621 gcctggtcgc cgtggcggtc gacacgcagg tgtggggcat ccgcacgctg gagatcgcga
     1681 tcttcgtgct gttcgtgttg gacaacccgc ggatgccgtt cttcttcggt ctgcccgcgt
     1741 ggattctggc ggtcgtcatc gtcggcatcg acattctcca actgttgggc aaccgccaga
     1801 ccggctacct gctggtgtac ctggtggcgt tggcgaccgc ggcgttggtg gcgcgttgct
     1861 tcggcatgct ggccgactac ccgtggctgc cgcagatcgt cgaccgcagc ggctcccgac
     1921 gccgcgtccg ccaacgcacg gggcgcgccg atgcgtcgca ccagaccgtg gtcagtgggc
     1981 cgtggccgag cgccccgccc acctacaccc cgatgcagga ccaggccgag gtcgaccgca
     2041 tcctcgacaa gatcgccgcg gtcggcatgg acggcctgac cgccgacgag aagaggcgcc
     2101 tcaacgaagc cagcaaacgc ctccgcaaag gcaccaacta ggtcgctcgg acgcgacccg
     2161 ctggagcaac gatgcaatgg atcgtctcgc cgatcgccgt gcgaccgatc ctgattgcca
     2221 tgttcggtct cgcgacagcg tgcacgtcgg gcgccagcga accggttgga cgccccgacg
     2281 cggagccact gccgatcagt gttctgtatc gaactgaacg gcagtacttc catacgatcg
     2341 aagacatctc tgctgcgagt gatgttgtca tcatcgcgac cgtcgtcagc gtcgaccccg
     2401 ccgacgttcc gcgtccggct gatgaaccgg atggctccgg aaccgagttc gtccgcctgt
     2461 cgctgcgtgc agacaccgtg ctgaagggcg acgttccgag cgaacttggc gtctcgtggg
     2521 aggcatacgt cgtcgacaac ggtgcacgca cgctcgagat tgcgatcgat ggcgtcgtcg
     2581 caccgaggac gtctgaccag gtcctgctct tcctgaagtg ggccgatcca agccagggag
     2641 cgctcggaat caccccggag tcgccgtggt acatcgtcac gatggacggc atctactgga
     2701 tcgacggaga caccgtcacg tcccctatca ccgacccgaa gcgcctggca tcgacagtga
     2761 acggtcaatc cctcgccgac atcgtcgcag cgatctcagc caccccgtca ggatgacgac
     2821 cgcccgttaa ctggcctcag ctgcggaggg ctttggtctt ggcggcggat ggtttcttgg
     2881 ttggcgtggc cttggccggg gtcttggccg c
//
LOCUS       DAORNF010000419         2911 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_25824_length_2911_cov_1.548319, whole genome shotgun sequence.
ACCESSION   DAORNF010000419 DAORNF010000000
VERSION     DAORNF010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2911
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25824_length_2911_cov_1.548319"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            134..865
                     /gene="ybeY"
                     /locus_tag="PLV68_15005"
     CDS             134..865
                     /gene="ybeY"
                     /locus_tag="PLV68_15005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HRE02608.1"
                     /translation="MQGGAIGMGDSSRPRLAARPKVGGDDEPEVFCADEQADLVVDLA
                     RWQRLAEDVLRAQGVRGGTELSIVFVGRDEIAELNESFLGKTGPTDVLSFPIDAAEVE
                     VVSSPSGATRGPDRAPSDPADLPLLLGDVVVCPAVAAEQAPAHAGTLDDELALLIVHG
                     ILHVLGHDHAEPEETRVMRARELELLERLHWGGPAPAGFRQAQPGDEADVLVDVVADA
                     VADVVADAAGAVADAADVGSEDDPS"
     gene            862..>1371
                     /locus_tag="PLV68_15010"
     CDS             862..>1371
                     /locus_tag="PLV68_15010"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF21 domain-containing protein"
                     /protein_id="HRE02609.1"
                     /translation="MIAARFNSADLWMIVAIAALLVLLAFLAVAEMGLSRISKQKAAA
                     IAERGTRSAAVLERLVTNPERWVNPLLLTVFIAQIVQSTLTAVVLDRLFGPLGVVIGV
                     FVNILVFFVLAEAVPKTWAVQHAEKAALLTARPLSALVALPPLRWFSRLLIWVTNVVV
                     RGKGLDKGPF"
     assembly_gap    1372..1473
                     /estimated_length=102
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1474..>1772
                     /locus_tag="PLV68_15015"
     CDS             <1474..>1772
                     /locus_tag="PLV68_15015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRE02610.1"
                     /translation="KEIMVPRPDMVVVASAVTVTEALDVAINRGVSRLPVLSEHNEDD
                     VIGVAYTKDLMRLERDGQGAQAVSDVARTAHVVPETKPVARLMREMQSQKFHMAF"
     assembly_gap    1773..1861
                     /estimated_length=89
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1862..>2170
                     /locus_tag="PLV68_15020"
     CDS             <1862..>2170
                     /locus_tag="PLV68_15020"
                     /inference="COORDINATES: protein motif:HMM:NF015436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HRE02611.1"
                     /translation="DEVERLDDGVYLVDGGMNITDFNTMMALDLPDDDWETVGGFVFA
                     TLEHVPDVGESVEYQGWTFAAEVVEGRRIRLLRVTVNPPDDADAPPGPADAEPETAVP
                     A"
     assembly_gap    2171..2235
                     /estimated_length=65
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2259..>2601)
                     /locus_tag="PLV68_15025"
     CDS             complement(2259..>2601)
                     /locus_tag="PLV68_15025"
                     /inference="COORDINATES: protein motif:HMM:NF024591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02612.1"
                     /translation="GRLHGEGRRGDVIITGGENVWPDPVQAAIAAHPAVADVAVAGVP
                     DPEWGQAVAAWIVVRDGHTLPTVAELRALVTQTLPAFAVPKVVRRAATLPRTALGKLR
                     RHELTATAHHH"
     assembly_gap    2602..2616
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2617..>2911)
                     /locus_tag="PLV68_15030"
     CDS             complement(<2617..>2911)
                     /locus_tag="PLV68_15030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02613.1"
                     /translation="SLVATALARVDPTPLRWIVLGGARPPADRPANTVTTYGMTETAS
                     GVVYDGLALNGVSMRIDRPDADGLGHIRISSPTLLRCYRDGTDPVDVEGYLATG"
BASE COUNT          371 a          869 c          966 g          434 t
ORIGIN      
        1 gctcaccttc gttcatctcg gtgccggtga cgtggtacgc cacaagatcg tcgccgacat
       61 cgtcgccgcc tacgagcagg ccgagcccat cgccttgccg cgccagcggc ggccgcggtg
      121 accgtgacgg tggttgcagg gcggagcgat cggcatgggt gattcgtccc gacctcgcct
      181 cgctgcacga cccaaggtcg gcggcgacga cgaacccgag gtgttctgcg cggacgagca
      241 ggcggaccta gtcgtcgatc tggcccgctg gcagcgtttg gccgaggacg tgttgcgcgc
      301 ccagggtgtg cgcggcggta ccgagttgtc gatcgtgttc gtcggccgcg acgagatcgc
      361 cgaactcaac gagtcgttcc tcggcaagac cggccccacc gacgtgctct cgtttcccat
      421 cgacgcggcc gaggtcgagg tcgtgtcgag cccgtcgggc gcgacgcgcg gccccgatcg
      481 ggcaccgtcc gacccagccg atctgccgct gttgttgggc gatgtggtgg tctgtcccgc
      541 ggtcgcggcc gaacaggcac cggcccacgc gggcacgctc gacgacgagt tggcgctgtt
      601 gatcgtgcat ggcatcttgc acgtgctcgg tcacgaccac gccgagccgg aggagacccg
      661 cgtgatgcgg gcccgcgaac tggaactgct ggagcggctg cactggggcg gcccggcacc
      721 ggccggcttc cgccaagccc aacccggcga cgaagccgat gtgctcgtcg atgtagtcgc
      781 cgatgcggtc gccgatgtgg tcgccgacgc cgcgggtgct gtcgccgacg ctgctgacgt
      841 tggcagcgag gatgatccgt cgtgatcgcc gcccggttca actctgcaga cctgtggatg
      901 atcgtggcca tcgccgcgct gttggtgttg ttggcgttcc tcgcggtggc cgagatgggc
      961 ctatcgcgca tctccaagca gaaggccgct gcgatcgcgg aacgaggcac ccgttcggcg
     1021 gcggtgctcg aacgcctcgt caccaacccg gagcgctggg tcaacccgtt gctgctgacc
     1081 gtgttcatcg cccagatcgt gcagtcgacg ctgaccgcgg tggtgctcga ccgcctgttc
     1141 ggcccgctcg gggtcgtgat cggtgtgttc gtcaacatcc tggtgttctt cgtgttggcc
     1201 gaagcggtgc ccaagacgtg ggcggtgcag cacgccgaga aggccgcgct gctgaccgcc
     1261 cggccgttgt ccgcgctggt cgcgctcccg ccgttgcgct ggttcagccg gttgctgatc
     1321 tgggtcacca acgtggtggt ccgcggcaag gggctcgaca aggggccgtt cnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnccaagga gatcatggtg ccgcgccccg
     1501 acatggtggt ggtcgcctcg gccgtcacgg tgaccgaggc cctcgacgtg gcgatcaacc
     1561 gcggtgtcag ccggttgccg gtgctcagcg aacacaacga ggacgacgtg atcggcgtcg
     1621 cctacaccaa ggacctgatg cgcctggagc gtgacggcca gggtgcccaa gcggtgtccg
     1681 acgtggcccg cacggcacac gtcgtgcccg agaccaagcc ggtcgcgcgg ttgatgcgcg
     1741 agatgcagtc acaaaagttc cacatggcct tcnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 ncgacgaggt cgagcgtctg gacgacggtg tgtacctggt cgacggcggc atgaacatca
     1921 ccgacttcaa cacgatgatg gccctcgatt tgcccgacga cgattgggag accgtcggcg
     1981 ggttcgtgtt cgccacgctc gaacatgtcc ccgacgtcgg cgagagcgtc gagtaccaag
     2041 gctggacctt cgctgccgaa gtcgtcgaag gccgccgcat ccggctgttg cgcgtcaccg
     2101 tcaacccccc cgacgacgcc gacgcgccac ctggccccgc ggatgccgag cccgagactg
     2161 ccgtcccagc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnccggc gtccgctgcg gcgggatctc agtgatggtg ggcggttgcc
     2281 gtgagttcgt ggcggcgcag tttgcccaag gctgtgcggg ggagggttgc tgctcggcgg
     2341 acgaccttgg ggacggcgaa ggcggggagg gtttgggtga ccagggcgcg gagctcggcc
     2401 accgttggca gcgtgtggcc gtcgcggacg acgatccagg cggcgacggc ctggccccac
     2461 tcggggtcgg ggactccggc aacggcgacg tcggccacgg cggggtgtgc ggcgatcgcg
     2521 gcctgaaccg ggtcgggcca cacgttctcg ccgccggtga tgatcacgtc gccgcggcgg
     2581 ccctcgccat ggagacggcc gnnnnnnnnn nnnnnngccg gtggccaggt agccctcgac
     2641 atccaccggg tcggtgccgt cgcgatagca gcgcagcagg gtgggggagg agatacggat
     2701 gtgccccagt ccgtcggcgt cgggtcgatc gatgcgcatc gagacgccgt tgagcgccag
     2761 cccgtcgtac acgacgccgc tggccgtctc ggtcatgccg taggtggtca ccgtgttggc
     2821 ggggcgatcg gctggtggcc gggcgccgcc gagcacgatc catcgcagcg gtgtcgggtc
     2881 gacccgggcc agcgcggtgg cgaccaacga g
//
LOCUS       DAORNF010000420         2908 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_25860_length_2908_cov_1.676831, whole genome shotgun sequence.
ACCESSION   DAORNF010000420 DAORNF010000000
VERSION     DAORNF010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2908
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25860_length_2908_cov_1.676831"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="PLV68_15035"
     CDS             complement(<1..300)
                     /locus_tag="PLV68_15035"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF014316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HRE02614.1"
                     /translation="MEGRVVAREMLTGDSVDVAPWLLNKVLVHGECAARIVEVEAYRH
                     DDPASHSYRGRTTRTKAMFGPAGHLYVYFTYGMHWCANVVTGADGEGSAVLLRAVV"
     gene            complement(345..752)
                     /locus_tag="PLV68_15040"
     CDS             complement(345..752)
                     /locus_tag="PLV68_15040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02615.1"
                     /translation="MNAGRWIARGVVAGVACVIPAITTSGCAECEAKCAGSHVEIAVG
                     GDVAQVTACDAAGRCSKQSFGPPGAAVIDRSFTVQVTDTGKRLTLTLSGVSVTGAPIP
                     ETVVQARASKGDCGCPGPAKMYITASAAGPFGD"
     gene            complement(795..>1973)
                     /locus_tag="PLV68_15045"
     CDS             complement(795..>1973)
                     /locus_tag="PLV68_15045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="steroid 3-ketoacyl-CoA thiolase"
                     /protein_id="HRE02616.1"
                     /translation="KSNVVIIDAVRTPIGKRNSGLSTLHLAELLGTVQKALIERTGID
                     PAIVGQVVTGCVSQVGEQAFNIGRTAWLSAGLPMTVANTTVDTQCGSSQQATGLATAL
                     VAAGVIDVAIACGVESMSRVPIGAAGNKALGLGIPIPKTYFDEYEMTSQFEGAERIAD
                     KWGITRADTDAFGLASQQRAIRAWEEDRFAGQYVAVTAPDRGEDGQLTDTTHTVTKDE
                     GLRETTAEKLASLKPVARDNGVHTAGNSSQISDGAAAVLIMSEEKAAELGLTPRARVV
                     DTCLVGTDPVLMLTGPIDATELLLQRSGLSIDDIDVFEINEAFASVVLAWAKETGADL
                     AKTNPNGGAIALGHPLGGTGAFLVTKALYELERIEGQYALVSMCCGGGLGTGTLLERI
                     "
     assembly_gap    1974..2023
                     /estimated_length=50
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2024..>2502
                     /locus_tag="PLV68_15050"
     CDS             <2024..>2502
                     /locus_tag="PLV68_15050"
                     /inference="COORDINATES: protein motif:HMM:NF020857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH pyrophosphatase zinc ribbon
                     domain-containing protein"
                     /protein_id="HRE02617.1"
                     /translation="PEHRSSFVALVVPPQNLPDQQRYVHVVGASVWVSDAPADGSWGR
                     HFLGVMNGAAVWAIDVPDGDDPTDGAALDLYSYFGRSSEPDWLMAGRGVQIVEWARTH
                     RFCGRCGTPNEPVERERAMRCPRCRLMAFPRLSPAMITLVTRGDGDDEQALLARGAQ"
     assembly_gap    2503..2614
                     /estimated_length=112
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2615..2830
                     /locus_tag="PLV68_15055"
     CDS             <2615..2830
                     /locus_tag="PLV68_15055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02618.1"
                     /translation="DDVRYQGSQPWPFPNSLMLGFRARYAGGEIECDATEIVDAQWFR
                     RDELPMIPPRISIARHLIDSWMEGRPA"
BASE COUNT          409 a          947 c          952 g          438 t
ORIGIN      
        1 caccaccgcg cgcaggagaa cagcggagcc ctcgccatcg gccccggtga ccacgttggc
       61 gcaccagtgc atgccgtacg tgaaatacac gtacaggtgc ccggccggcc cgaacatggc
      121 tttggtgcgc gtggtgcggc cgcggtacga gtggctcgcc gggtcgtcgt gccggtacgc
      181 ctcgacctcg acgatccgcg ctgcacactc gccgtgcacc agcaccttgt tcagcaacca
      241 cggcgccacg tcgaccgagt cccccgtcaa catctcccgc gcgaccaccc gaccctccac
      301 caaccccacc ctatgcctca gcattcgggc caaatgtggc cgggtcagtc gccgaagggg
      361 ccggcggccg aggccgtgat gtacatcttg gcggggccgg ggcagccgca gtcgcccttg
      421 ctggcgcggg cttggacgac ggtctcggga atcggtgcac cggtgacgga cacaccgctg
      481 agggtgagcg tgagcctctt gccggtgtcg gtcacctgaa cggtgaacga tcggtcgatc
      541 accgctgcgc ccggcgggcc aaagctctgt ttgctgcatc gcccggcggc gtcgcaggcg
      601 gtcacctggg ccacgtcgcc gccaaccgcg atctcgacat gcgagccggc acacttcgcc
      661 tcgcactcgg cgcacccact cgtggtgatc gccggaatga cacatgccac cccggcgacc
      721 acgccccgag ctatccagcg tccggcattc attgctcaca ctttgatgac ggccgcgccg
      781 acgctgcggt tgggttagat gcgttcgagg agggtgccgg tgccgaggcc gccgccgcag
      841 cacatgctga ccagggcgta ctggccctcg atccgctcca actcgtacag ggccttggtg
      901 accaggaacg caccggtacc accgaggggg tggccgagcg cgatcgcgcc gccgttgggg
      961 ttggtcttgg ccagatcggc accggtctcc ttggcccatg cgagcacgac cgacgcgaac
     1021 gcctcgttga tctcgaacac gtcgatgtcg tcgatcgaca gaccgctgcg ctgcaacaac
     1081 agctcggtgg catcgatggg gccggtcagc atcagcaccg ggtcggtgcc gaccaggcag
     1141 gtgtcgacga cgcgagcgcg cggcgtgagg ccgagctcgg cggccttctc ctcgctcatg
     1201 atcaacacgg ccgcggcgcc gtcgctgatc tggctggagt taccagcggt gtgcacgccg
     1261 ttgtcgcggg cgaccggctt gagcgacgcg agcttctcgg cggtggtctc gcgcaggccc
     1321 tcgtccttgg tgacggtgtg ggtggtgtcg gtgagctggc cgtcctcgcc gcggtcgggg
     1381 gcggtgaccg ccacgtactg accggcgaag cggtcttcct cccacgctcg gatcgcgcgc
     1441 tgctggctgg ccaggccgaa cgcgtcggtg tcggcgcggg tgatgcccca cttgtcggcg
     1501 atgcgctccg caccttcgaa ctggctggtc atctcgtact cgtcgaagta ggtcttgggg
     1561 atcgggatgc cgaggccgag cgccttgttg ccagcagcgc cgatcgggac ccgactcatc
     1621 gactcgacac cacaggcgat cgccacatcg atcacacccg cggcgaccag agccgtggcc
     1681 aggccggtgg cctgctggct ggaaccgcac tgggtgtcga ccgtggtgtt ggccaccgtc
     1741 atcggcagac ccgccgacaa ccatgcggtg cgaccgatgt tgaacgcttg ctcgcccacc
     1801 tggctgacgc agccggtgac gacctggccg acgatggcgg ggtcgatgcc ggtgcgctcg
     1861 atgagcgcct tctggacggt gccgagcagc tcggcgaggt ggagggtgga cagaccactg
     1921 ttgcgcttgc cgatcggcgt acgcacggca tcgatgatca ccacattgct cttnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnccagagc atcgcagttc
     2041 gttcgtcgcc ctcgtcgtcc cgccgcagaa cctgcccgac cagcagcgct acgtccacgt
     2101 cgtgggtgcg agtgtgtggg tgagcgatgc gccagcggac gggtcgtggg gtcgccactt
     2161 cctcggcgtg atgaacggcg cggcggtgtg ggcgatcgac gtgcccgacg gcgacgaccc
     2221 gaccgacggc gcggcgctcg acctgtacag ctacttcggg cggtcgagcg aacccgactg
     2281 gctgatggcc ggacgcgggg tgcagatcgt ggagtgggcc agaacccacc gcttctgcgg
     2341 ccgctgcggc acgcccaacg agccggtcga gcgagagcgg gcgatgcgct gcccgcgctg
     2401 tcggctgatg gctttccccc gcctgtcacc ggcgatgatc acgctggtca cccgcggcga
     2461 cggcgacgac gaacaagcgc tgctggcccg cggcgcgcaa ttnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngacgac gtgcgctacc agggcagtca
     2641 gccatggccg ttcccgaaca gcctgatgct ggggttccgg gcacgctacg cgggtggcga
     2701 gatcgagtgc gacgcgaccg agatcgtcga cgcacagtgg ttccggcgcg acgagcttcc
     2761 gatgatcccg ccgcgcatca gcatcgcccg ccacctgatc gactcctgga tggagggccg
     2821 ccccgcctga ccgggcctgc gtggcggggc ggcaactggc cgacagcgac cgccgaccgt
     2881 ccgtcagcgc gcggcgagtt tgtcggtg
//
LOCUS       DAORNF010000421         2904 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_25913_length_2904_cov_1.804142, whole genome shotgun sequence.
ACCESSION   DAORNF010000421 DAORNF010000000
VERSION     DAORNF010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2904
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25913_length_2904_cov_1.804142"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..949)
                     /locus_tag="PLV68_15060"
     CDS             complement(<1..949)
                     /locus_tag="PLV68_15060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRE02619.1"
                     /translation="MKRISHWIDGKVVEGTSGRSATVWQPATGEAQATVDLASVAEVD
                     HVVAVAKQAFAGWRSTNLSRRAEVMFNLRELIAANRKEIASLLSAEHGKVLSDALGEV
                     SRGLENIEFACGVPHLLKGSFSEQASTGVDVYNIRQPLGVVAGITPFNFPAMVPMWMF
                     ANALACGNTFVLKPSEKDPSSAMFLAELLKQAIEGRGDRWQRAEMLNELHARRLRLGI
                     IDRVASLVRHGHRTLRARVVGEHLHLPRRESAFEIIDDIFAGREVDLELGALVGAKRI
                     EAAVENRFSRGHQLQYDRLTVRQMVAHRGDHRRELHRQQH"
     gene            complement(1035..2441)
                     /gene="hydA"
                     /locus_tag="PLV68_15065"
     CDS             complement(1035..2441)
                     /gene="hydA"
                     /locus_tag="PLV68_15065"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HRE02620.1"
                     /translation="MARTLITNGIVVSPTGRQQLDVLIDGETIAAIYAPGQVEAQGVT
                     AERTIDASGKYVIPGGIDVHTHMELPFGGTNAVDTFEAWGGVTTIVDFAVQRTGENVI
                     DSLAAWHAKADGECAIDYGFHQIVGGVDEEALKAMRYLTDNEGVTSFKLFMAYPGVFY
                     SDDGKILQAMQTAGDIGATIMMHAENGIAIDVLVAQALGRGETDPKYHSYTRPSALEG
                     EATHRAIVLSQVAGKAPLYFVHVSASEALYEIAAARDSGYNVFAETCPQYLYMTLEET
                     LAKPGFEGAKWVCSPPIRSAHDTHKHHADLWKGLRTNDVAIVSTDHCPFCMKDQKELG
                     LGNFSAIPNGMGGVEHRMELLFEGVVNGEISLERWVETCCTTPARMFGMYPKKGIVAP
                     GADADIVLWDPNTQTHIGFEHKHHMNMDHSSWEGYIIDGKVDTVLSRGTVVIENDQYV
                     GRKGHGRFVKRGLSQYLV"
     gene            complement(2496..>2904)
                     /locus_tag="PLV68_15070"
     CDS             complement(2496..>2904)
                     /locus_tag="PLV68_15070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HRE02621.1"
                     /translation="AVGKVGVYICYDRHFPEGWRALGLNGAQIVFNPSATHRGLSEYL
                     WRLEQPAAAVANIYYVGAINRVGIEELGENDFYGQSYFVDPEGKFVGDVGDPYKPEVI
                     VRDLDMDKLAEIRDRWAFYRDRRPDAYDELVRR"
BASE COUNT          441 a          915 c          997 g          551 t
ORIGIN      
        1 ggtgctgttg tcgatgaagc tcccgacggt gatcgccgcg gtgcgcgacc atctggcgaa
       61 cggtcagtcg gtcgtactgc aactggtgac cacggctgaa tcgattctcg accgccgcct
      121 caatgcgctt agccccgacg agcgcgccga gctcgagatc gacctctcgc cccgcgaata
      181 tgtcatcgat tatctcgaac gcgctttccc tacgcggcag atgcaggtgt tcaccgacga
      241 cacgggcacg cagcgttcgg tgcccatggc ggacgaggct ggcaacccgg tctataatcc
      301 cgaggcggag gcggcgcgcg tgcagctcat tgagcatctc tgcgcgctgc caccgatcac
      361 ctctgccctc gatggcctgc ttgagcagtt cggccaggaa catcgccgac gacgggtcct
      421 tctcggacgg cttgagcacg aaggtgttgc cgcacgccag cgcgttggcg aacatccaca
      481 tgggcaccat cgcggggaag ttgaacgggg tgatgccggc gaccacgccg aggggctggc
      541 ggatgttgta gacgtcgaca ccggtcgagg cctgctcgga gaacgacccc ttcaacaggt
      601 ggggcacgcc gcaggcgaac tcgatgttct ccaggccgcg agacacctcg cccagcgcgt
      661 cggacagcac cttgccgtgc tcggccgaca gcagcgacgc gatctccttg cggttggcgg
      721 cgatgagctc acgcaggttg aacatcacct cggcccggcg cgacaggttg gtcgagcgcc
      781 atccggcgaa ggcctgcttg gcgacggcga ccacgtggtc gacctcggcc accgatgcca
      841 ggtcgaccgt cgcctgtgct tcgcccgtgg cgggctgcca caccgtggcc gagcggcccg
      901 aggtgccttc gacgaccttg ccgtcgatcc agtgactgat gcgcttcatg tgatgtcctt
      961 acttgctctg cttgttgctt gcgactcgtg gagtcgtgag ttctcggtgc ctgacgggac
     1021 cagtgggtgt ggtgtcagac caggtattgg gacaggccgc gcttgacgaa gcggccgtga
     1081 cccttgcgcc cgacgtactg gtcgttctcg atcacgaccg tgccgcggga cagcaccgtg
     1141 tcgaccttgc cgtcgatgat gtagccctcc cacgaggagt ggtccatgtt catgtggtgc
     1201 ttgtgctcga agccgatgtg ggtctgcgtg ttggggtccc acaacacgat gtcggcgtcg
     1261 gcgccggggg cgacgatgcc cttcttgggg tacatgccga acatccgcgc cggggtggtg
     1321 cagcacgtct cgacccaccg ttcgaggctg atctcgccgt tgaccactcc ctcgaacaac
     1381 agctccatcc ggtgctcgac gccacccatg ccgttgggga tggcggagaa gttgcccagg
     1441 ccaagctcct tctgatcctt catgcagaac gggcagtggt cggtggacac gatcgcgacg
     1501 tcgttggtgc gcaggccctt ccacagatcg gcgtggtgct tgtgggtgtc gtgggcgctg
     1561 cggatcggcg gtgagcacac ccacttggca ccttcgaaac caggcttggc caacgtctct
     1621 tcgagggtca tgtagaggta ctgcgggcag gtctcggcga acacgttgta gcccgagtcg
     1681 cgcgctgcgg cgatctcgta cagcgcctcg ctggccgaga cgtggacgaa gtacagcggt
     1741 gccttgcccg ccacctggct gagcacgatc gcccggtggg tggcttcacc ctcgagcgcg
     1801 gacgggcggg tgtagctgtg gtacttcggg tcggtttcgc cccggccgag cgcctgcgcg
     1861 accaggacgt cgatggcgat gccgttctcg gcgtgcatca tgatggtcgc gccgatgtcg
     1921 cccgcggtct gcatcgcctg caggatcttg ccgtcgtcgc tgtagaacac gccggggtag
     1981 gccatgaaca gcttgaagct ggtgacgccc tcgttgtcgg tgaggtagcg catcgccttg
     2041 agcgcttcct cgtcgacgcc gcccacgatc tggtggaagc cgtagtcgat cgcgcactcg
     2101 ccgtcggcct tggcgtgcca tgcggccagg ctgtcgatca cgttctcgcc ggtgcgctgc
     2161 accgcgaagt cgacgatggt ggtgacaccg ccccacgcct cgaaggtgtc gaccgcgttg
     2221 gtgccgccga agggcagctc catgtgcgtg tgcacgtcga tgccgccggg gatgacgtac
     2281 ttgcccgacg cgtcgatggt gcgctcggcg gtgacgcctt gcgcctcgac ctggcctggg
     2341 gcgtagatcg cggcgatggt ctcgccgtcg atcaagacgt cgagctgctg gcggccggtg
     2401 gggctgacga cgatgccgtt ggtgatgagc gtgcgggcca tgagcgttct cctgctggtc
     2461 gaagcggtga tctgagcggg gcggatgcgg gttggtcagc gacggacgag ttcgtcgtac
     2521 gcgtcgggac gacgatcacg gtagaacgcc caccgatcac gaatctcggc gagcttgtcc
     2581 atgtcgaggt cgcgcacgat gacctcgggc ttgtacgggt caccgacatc gccgacgaac
     2641 ttgccctcgg ggtcgacgaa gtaactctgc ccgtagaagt cgttctcgcc gagctcttcg
     2701 atgccgaccc ggttgatcgc cccgacgtag tagatgttgg ccacggctgc ggcgggctgt
     2761 tccagccgcc acaggtattc gctgaggccg cggtgggtgg cgctggggtt gaacacgatc
     2821 tgcgcgccgt tgagcccgag cgcgcgccag ccttcgggga agtggcggtc gtagcagatg
     2881 tagacgccga ccttgccgac cgcg
//
LOCUS       DAORNF010000422         2904 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_25914_length_2904_cov_1.793963, whole genome shotgun sequence.
ACCESSION   DAORNF010000422 DAORNF010000000
VERSION     DAORNF010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2904
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25914_length_2904_cov_1.793963"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    264..331
                     /estimated_length=68
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<332..>628)
                     /locus_tag="PLV68_15075"
     CDS             complement(<332..>628)
                     /locus_tag="PLV68_15075"
                     /inference="COORDINATES: protein motif:HMM:NF005858.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02622.1"
                     /translation="GRASVDIIKTGGYKVGAGEVETALLAHPAVAETAVIGVADADLG
                     QRVVAYVVADAVTTAELDRFLAEQLTTHKRPREIRIVASLPRNAMGKVLKSELAA"
     assembly_gap    629..704
                     /estimated_length=76
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<705..1726)
                     /locus_tag="PLV68_15080"
     CDS             complement(<705..1726)
                     /locus_tag="PLV68_15080"
                     /inference="COORDINATES: protein motif:HMM:NF005858.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRE02623.1"
                     /translation="MSVLLPGLSGMGAGGDRVDSVSVGGAALSFEALAARAHTVGAAI
                     TGAGVVAVDATATLDTVVAVCAGLAAGVAVVPVPPDAGPVERAHILRDSSATAVLGAP
                     DWASVDLPRIDLTTAIGSGSTALPEPPSDATALIMYTSGTTGLPKGAVLSRRAIAAGL
                     DGLAEAWGWTADDHLVHGLPLFHVHGLILGVLGALRVGSRLTHTGRPTPAAYAAAAGS
                     LYFGVPTVWSRVCAEPDAAAALHRARLLVSGSAPLPVPVFDRLHELTGHRPVERYGMT
                     ETLITLSTRADGERRPGWVGLPIAGVQTRLVADDGTAVDHDGETIGGLQVRGRTLFDG
                     YLTGGRP"
     gene            1914..2375
                     /locus_tag="PLV68_15085"
     CDS             1914..2375
                     /locus_tag="PLV68_15085"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRE02624.1"
                     /translation="MRQSGDGFMQCSDGAVRWGRFGAAGVVFVTQVDDVPHVMLQLRS
                     RWAHEGGTWSCAGGAIDEGEEPYEAALREASEEVGEVPADHRLLGEYVFAPATDWRYT
                     TVVIGVDAPFGASVNFETDDVAWVPVQAVDQRPLHAGFAAAWPHLRAIIES"
     gene            2430..>2904
                     /locus_tag="PLV68_15090"
     CDS             2430..>2904
                     /locus_tag="PLV68_15090"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRE02625.1"
                     /translation="MNNPDYTPLASAPPWFAKALRVPFDSFTTQVGGVPINALTWGQS
                     GKRGLVFVHGGNAQADWWAHIAPWFADEFRVVALDLSGHGDSGWREQYGLEQWTDEVL
                     AVADAGGIDGPPVVVGHSMGGMVSIVAAARHAERIGGVIVIDSPVAEVDSEIQAHR"
BASE COUNT          410 a          957 c          979 g          414 t
ORIGIN      
        1 gcttgccgcg gtcgaggcgt ggctgatggc agcgctcggc gcgtcaggcc caggtacgcc
       61 cgcaccgacg acgctgacaa ccgtggtgac ggcagcgggt gatgcggtga gtgagggtgc
      121 cgtcgctggt gagcacgacg ctggcggtga gggcgatgcg gcccgtgccg ctgatcgctt
      181 gcaccgcctg atcgcagcgc tgtcggccga cgactggcag cgcacggtga cccacccgac
      241 actcggtgtt gcgactatcc aggnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nggcggcgag ctccgacttg aggactttgc
      361 ccatcgcgtt gcggggcagg ctggcgacga tgcgaatctc gcgggggcgc ttgtgcgtgg
      421 tcagttgttc cgcgagaaag cggtcgagtt ccgctgtggt gaccgcgtcg gcaaccacat
      481 aggcgacgac ccgctggccg aggtcggcat cggcgacacc gatcacggct gtctcggcca
      541 cagcggggtg ggcgagcagc gcagtctcga cttcacccgc gccgaccttg tagccgccgg
      601 tcttgatgat gtcgaccgac gcccgtccnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnggccgc ccgccggtga
      721 ggtagccgtc gaacagcgtc ctgccgcgta cctggagtcc gccgatcgtc tcgccgtcgt
      781 ggtcgaccgc ggtgccgtcg tccgcgacca ggcgcgtctg cacgccggcg atcggcaggc
      841 cgacccatcc cgggcggcgc tcgccgtcgg cgcgggtgct cagtgtgatc agggtttcgg
      901 tcatgccgta gcgctcgact gggcggtggc cggtgagctc gtgcaagcgg tcgaacacgg
      961 gcacgggcaa cggcgcgctt cccgacacca acagccgcgc gcggtgcagc gccgcagcag
     1021 cgtctggttc agcgcagacc ctcgaccaca cggtgggcac cccgaagtac agactgcccg
     1081 ctgcagccgc ataggcggcc ggcgtcggcc gccccgtgtg ggtcaggcga ctgccgacgc
     1141 gcagcgcgcc gagtacgccc aggatcaatc cgtgcacatg gaacagtgga agtccgtgga
     1201 caagatgatc atcggccgtc cagccccacg cttcggccaa tccgtcgagc ccagcggcga
     1261 tcgcccgccg tgacagcacc gctcccttgg gcagtcctgt ggtaccggag gtgtacatga
     1321 tcagcgcggt cgcgtcggac ggtggctcgg gcagcgctgt cgagcccgat ccgatcgctg
     1381 tcgtgagatc gattcgcggt aggtcgacgc tggcccagtc gggggcaccg agcaccgccg
     1441 tggcgctgct gtcgcgcagg atatgcgccc gttcgaccgg cccggcatcc ggcggtaccg
     1501 gtacgaccgc aacgcccgcc gccagtccgg cgcacaccgc gaccacggtg tcgagcgtgg
     1561 ccgtcgcgtc gaccgcaacc acgcctgccc ccgtgatcgc tgccccaacc gtgtgcgcgc
     1621 gtgccgccag cgcttcgaac gacagcgctg caccgccaac gctcaccgag tcgacccgat
     1681 cgccgcccgc ccccatcccc gacaacccag gcagcaacac gctcatcggc ccgacgctac
     1741 tgacccggca ccatctggcg cccgcccacc ccggtgaatc cagcgtccgc ggaacgggga
     1801 ttgcccggat tccggcaaac ccgtactccg cggaagccag accgcccggc ccccggcccg
     1861 gcgatgctgg cgtctggtgg gttcggaggg ggccgacgtg gtacacacgg gtgatgcgcc
     1921 aaagtggaga cgggttcatg cagtgcagcg acggcgccgt gcggtggggc cgcttcgggg
     1981 cggccggagt cgtgttcgtc acccaggtcg acgacgtccc ccacgtgatg ctgcagttgc
     2041 ggtcacgctg ggcacacgag ggcggcacgt ggtcgtgtgc aggtggcgcg atcgacgagg
     2101 gcgaagagcc gtacgaggca gcgttgcgcg aagcgtccga agaagtgggc gaggtgccgg
     2161 ccgaccaccg cttgctcggt gaatacgtct tcgctccggc gaccgactgg cgctacacca
     2221 ccgtggtgat cggcgtcgac gccccctttg gcgcgtcggt caacttcgag accgacgacg
     2281 tcgcgtgggt gccggtgcag gcggtcgacc agcgtccgct gcacgccggg tttgccgcag
     2341 catggcccca tctccgcgcg atcatcgagt cgtagtcacg gcgatcatcg agtcatagtc
     2401 acgcagacct gcccgaatac ggtggcggtg tgaacaaccc ggactacacc ccgctcgctt
     2461 cggcaccgcc gtggttcgcc aaggcgctgc gggtgccgtt cgattcgttc accacccagg
     2521 tcgggggagt cccgatcaac gcgctgacgt ggggacagtc cggcaagcgc ggcttggtgt
     2581 tcgtccacgg cggcaacgcg caggctgact ggtgggcaca catcgcgccg tggttcgccg
     2641 acgagttccg ggtggtcgcc ctcgacctca gcggccacgg cgacagcggt tggcgcgagc
     2701 aatacggcct cgagcagtgg accgacgagg tcttggccgt ggccgatgcg ggcggcatcg
     2761 acggtcctcc cgtcgtcgtc ggccacagca tgggcggcat ggtgtcgatc gtcgccgcag
     2821 cgcgccacgc cgagcggatc ggcggggtga tcgtgatcga ttcgccggtg gccgaggtcg
     2881 actccgagat ccaagcccac cgtc
//
LOCUS       DAORNF010000423         2886 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_26159_length_2886_cov_1.481102, whole genome shotgun sequence.
ACCESSION   DAORNF010000423 DAORNF010000000
VERSION     DAORNF010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2886
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26159_length_2886_cov_1.481102"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="PLV68_15095"
     CDS             <1..210
                     /locus_tag="PLV68_15095"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HRE02626.1"
                     /translation="YARIAARHFGVDHREYYVTPADLLDGIPRVLTCRVRSETVEIRR
                     PGSRGRLRWVQMRDVAVTWEQLEMW"
     gene            276..>1266
                     /locus_tag="PLV68_15100"
     CDS             276..>1266
                     /locus_tag="PLV68_15100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02627.1"
                     /translation="MGVRLDPSDEYMHELGPEPNFNESMYINCFDPDQQVGGWFRIGN
                     RANEGYAEMTVCLYLPPDSTEDGSSARQVAFMYKRPKIDNNDAFDAGGMTWTMVTPFE
                     ELRVAYSGRVVVLDDPLQMAEPKLAFTENPHVDCEVQLTFTGQGRASMFGGEPDEAHE
                     APGEEFAKGHYEQLVQATGTIRVGERSWTIAGYGLRDHSWGPRYWQAPWYYRWLTANF
                     GRDLGFMASRVAKKDGPGTRGGFVWDDGQLHLCDDVEISTEFTGPDTYHRRIDGVLRS
                     SRSDRSWTFTGEVIDLIPLRNRRETPDGDMLTTRISEGMTRWTMQRAGDPSSGE"
     assembly_gap    1267..1436
                     /estimated_length=170
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1437..>1675)
                     /locus_tag="PLV68_15105"
     CDS             complement(<1437..>1675)
                     /locus_tag="PLV68_15105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02628.1"
                     /translation="EFVTHFVSDVRDDADPAGGSVHIHCTDVAGEWTLLPGDPPAQAG
                     EYVVTREHSKCDCAMRGSASDLLLVLWRRIGIDQI"
     assembly_gap    1676..1789
                     /estimated_length=114
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1790..2159)
                     /locus_tag="PLV68_15110"
     CDS             complement(<1790..2159)
                     /locus_tag="PLV68_15110"
                     /inference="COORDINATES: protein motif:HMM:NF023144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HRE02629.1"
                     /translation="MNPTTYGDASDHIAALEREIAAFRVAVTEMPLDRAVPQLGSGPG
                     AGTGWSVADLIHHMTQVQYFWGEVVRQRATGWKQVAALARAADDALIAAFDEHHATLV
                     ATLGATPPDTRVWTWSDQQDV"
     gene            2208..>2614
                     /locus_tag="PLV68_15115"
     CDS             2208..>2614
                     /locus_tag="PLV68_15115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02630.1"
                     /translation="MSQAQPPADGASSTTAASEAIAAAVIGGVVGASSPAVSPDGTQI
                     AFVVTRTDLDKNTYRSQVWLTPADASRPPRPITSGEHNDSQPVWAPDGSALAFVSSRS
                     PKKGEATLHLLPIDGPGEVRTVATMPDGIGSVS"
     assembly_gap    2615..2624
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2625..>2886
                     /locus_tag="PLV68_15120"
     CDS             <2625..>2886
                     /locus_tag="PLV68_15120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02631.1"
                     /translation="SPDGRQIAFTSRTQDERYKAEDESWQSPRKIERFFTRLNGEGWV
                     FDRPTHVYVVPTDGTAAPRNLTPGPFEHGGVAWLTDGSAVITA"
BASE COUNT          442 a          922 c          859 g          369 t
ORIGIN      
        1 tacgcccgca tcgccgcgcg gcacttcggc gtggatcacc gcgaatacta cgtcacgccc
       61 gccgacctgc tcgacggcat cccgcgcgtg ctgacttgcc gggtccgctc agagaccgtg
      121 gagattcgcc gacccggctc gcgtggccgg ctccgatggg tgcagatgcg cgacgttgcc
      181 gtcacgtggg aacagctgga gatgtggtga gcacgacggg accccacgtt gggcccgcct
      241 cggccacgcg gcagcggccc ccgtagtgtg agcccatggg agtccgcctc gacccgtccg
      301 acgaatacat gcacgaactg ggccccgagc ccaacttcaa cgagagcatg tacatcaact
      361 gcttcgatcc cgaccagcag gtgggcggat ggttccgcat cggcaaccgg gccaacgaag
      421 ggtacgcgga aatgaccgtc tgcctctacc tgccgcccga ctcgaccgaa gacgggagca
      481 gcgcccggca ggtcgcgttc atgtacaagc gacccaagat cgacaacaac gacgccttcg
      541 atgcgggagg catgacctgg acgatggtca ccccgttcga agagctccgg gtcgcctact
      601 ccggccgggt cgtggtgctc gacgacccgc tgcagatggc cgagcccaag ctggccttca
      661 ccgagaatcc ccacgtcgat tgcgaggtgc agttgacctt cacgggccag ggccgggcca
      721 gcatgttcgg cggcgagccc gatgaggccc acgaggcacc gggcgaggag ttcgccaaag
      781 gtcactacga gcagttggtg caagcgaccg gcacgatccg agtcggcgag cgcagctgga
      841 ccatcgccgg ctacgggttg cgcgaccact cgtgggggcc gcgctactgg caggccccct
      901 ggtactaccg ctggctgacc gccaacttcg gccgcgatct cggcttcatg gccagccgcg
      961 tcgccaagaa ggacgggccg ggcacccgtg gcggcttcgt gtgggacgac ggccagttgc
     1021 acctgtgcga cgatgtcgag atcagcaccg agttcaccgg tcccgacacc taccaccggc
     1081 gcatcgacgg tgtgttgcgc tcgtcgcgca gcgatcggtc gtggaccttc accggcgagg
     1141 tgatcgacct gatcccgttg cgcaaccggc gcgagacccc cgacggcgac atgctgacca
     1201 ctcgcatcag cgagggtatg acgcgctgga cgatgcagcg cgcgggcgac ccaagctcgg
     1261 gcgagannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncgat
     1441 ctgatcgatg ccgatgcggc gccacaggac cagcagcagg tcgctggcac taccgcgcat
     1501 cgcgcagtcg cacttggagt gctcgcgggt gacgacgtac tcgccggctt gggcaggcgg
     1561 gtcgccgggc agcagcgtcc actcgccggc gacgtcggtg cagtggatgt gcaccgatcc
     1621 accggccggg tcggcgtcgt cgcgcacgtc ggagacgaag tgggtgacga actcgnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc gacgtcctgc
     1801 tgatcgctcc acgtccacac ccgggtgtcg ggaggggtcg cgccaagcgt ggccaccaac
     1861 gttgcgtggt gctcgtcgaa tgctgcgatc aaggcgtcgt cggcggctcg cgccagcgcg
     1921 gcgacctgct tccatcccgt cgcccgctga cggacgacct cgccccagaa gtactgcacc
     1981 tgtgtcatgt gatggatcag gtcggcgacc gaccaccccg tccccgcacc cggccccgaa
     2041 cccaactggg gcaccgcccg gtcgagcggc atctcggtga ccgccacccg gaacgcagcg
     2101 atctcccgct ccagggccgc gatgtggtcg gaggcatcgc cgtaggtcgt gggattcatc
     2161 gcttcatctg accacagcgc cgctccgagt ggctaccgtc ggaacatatg agccaagcac
     2221 agccccccgc cgacggtgcc tcgtccacaa ccgctgcatc agaagcgatc gccgcggcag
     2281 tgatcggagg cgtcgtcggc gcctccagcc cggcggtgtc gccggacggc acccagatcg
     2341 cgttcgtcgt cacccgcacc gacctggaca agaacacgta ccgcagccag gtgtggctga
     2401 cccccgccga cgccagtcgc ccaccgcggc cgatcaccag cggtgagcac aacgactcgc
     2461 aaccggtctg ggcgcccgac ggatcggcgc tggcgttcgt gtcgtcgcgc agccccaaga
     2521 agggcgaggc caccctgcac ctgctgccca tcgacgggcc gggcgaggtg cgtacggtgg
     2581 ccacgatgcc cgacggcatc ggcagcgtca gcttnnnnnn nnnncagccc cgacggccgc
     2641 cagatcgcct tcaccagccg tacgcaggac gagcgctaca aggccgaaga cgagagctgg
     2701 cagagcccgc gcaagatcga gcgcttcttc acgcggctca acggtgaggg ttgggtgttc
     2761 gatcgaccga cccacgtcta cgtcgtgccc accgacggca ccgccgcgcc gcgcaacctc
     2821 acacccgggc cgttcgaaca cggcggcgtc gcctggctga ccgatgggtc ggccgtgatc
     2881 accgcc
//
LOCUS       DAORNF010000424         2885 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_26168_length_2885_cov_1.905654, whole genome shotgun sequence.
ACCESSION   DAORNF010000424 DAORNF010000000
VERSION     DAORNF010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2885
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26168_length_2885_cov_1.905654"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="PLV68_15125"
     CDS             complement(<1..271)
                     /locus_tag="PLV68_15125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02632.1"
                     /translation="MSLRDRFFTPRVLRALISPWSVIAAVAVGAASVVAGVPLAAGIA
                     IAVGVYAVRVGVAMFTTDASTTGSKLRIDPFTLSEPWRRHVQAALN"
     gene            complement(268..1086)
                     /locus_tag="PLV68_15130"
     CDS             complement(268..1086)
                     /locus_tag="PLV68_15130"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRE02633.1"
                     /translation="MRTITDLMAELDQLVGLDTVKNEIAKLTSLLQIQALRAERGLPT
                     IETSHHLVFTGNPGTGKTTVARLLSQIYRSVGVVSKGQLVETDRSNLVAGYVGQTATK
                     TMAVLESALGGMLLIDEAYSLARGGPNDFGREAIDTLVKFMEDHRDDIGIVAAGYPAE
                     MQELIDTNPGLKSRFTKTIHFDDYTDDELVSIFDKLGAKNHYALTDEARDKLYAILAA
                     TSRDRGFGNARLVRNLFEDAVGRQAQRLAVLDEPTDEHLTTLVAADLPPIGAPL"
     gene            1181..1498
                     /locus_tag="PLV68_15135"
     CDS             1181..1498
                     /locus_tag="PLV68_15135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02634.1"
                     /translation="MLLPVVLLPVVLLPVVLSIVGAGLAAGSFGSDRGSAERAGTGHA
                     RCVHRLQHDQTEAFDVVEFGRRGDQVERRAQPGEAQRSIVVAVAASGAVAGIGAHQQV
                     EQH"
     gene            1883..2752
                     /locus_tag="PLV68_15140"
     CDS             1883..2752
                     /locus_tag="PLV68_15140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HRE02635.1"
                     /translation="MAPRFESVQSVREGLRDVNYLADEGIAGVAFLADRLGKPVLVEG
                     PAGTGKTQLAKSVAEMTGARLIRLQCYEGLDESKALYEWNYKKQLLRIQADKNSDSWS
                     TVHDDIFSDEFLLTRPLLEAITADDPVVLLIDEVDRVEVETEALLLEILSEYQVSIPE
                     LGTITAKQVPLVFLTSNNTRELSEALKRRCLFLHVDYPDMEREKQIVLTKVPGVSENL
                     ADQIARIVRSIRQLDLKKAPSVSETIDWARTLVLLGIDHIEAQQAKDTLHILLKYQTD
                     IAKAAKELSVDPK"
BASE COUNT          460 a          956 c          990 g          479 t
ORIGIN      
        1 cgttgagggc tgcctgcacg tgccggcgcc agggttcgct gagcgtgaac gggtcgatcc
       61 gcagcttgga cccggtcgtg ctggcgtcgg tggtgaacat cgccacgccg acgcgcaccg
      121 cgtacacgcc caccgcgatg gcgatgcccg ccgcgagtgg cacgccggcc actaccgagg
      181 cggcacccac cgccacggcg gcgatcaccg accacggcga gatgagggcg cgcagcacgc
      241 gcggcgtgaa gaagcggtcg cgcaggctca cagcggggcc ccgatcggcg gcagatcggc
      301 cgcgaccagt gtggtcaggt gctcgtcggt cggttcgtcc agcaccgcca gccgctgcgc
      361 ttggcgtccg acagcgtctt cgaacaggtt gcgcaccagg cgagcgttgc cgaagccgcg
      421 atcgcgcgag gtcgccgcca ggatcgcgta cagcttgtca cgagcctcgt ccgtgagcgc
      481 gtagtggttc ttggcgccga gcttgtcgaa gatcgacacg agttcgtcgt cggtgtagtc
      541 gtcgaagtgg atcgtcttgg tgaagcggct cttcaggccg gggttggtgt cgatcagttc
      601 ctgcatctcg gccggataac ccgccgcgac tatgccgatg tcgtcgcggt gatcttccat
      661 gaacttgacc agggtgtcga tcgcctcgcg gccgaagtcg ttggggccgc cacgggccag
      721 gctgtaggcc tcgtcgatca gcagcatgcc gccgagcgcg gactcgagca cggccatcgt
      781 cttggttgcc gtctggccga cataccccgc aacaaggttc gaccggtcgg tctcgaccaa
      841 ctgtcccttc gacaccacgc cgacgctgcg atagatctgg ctgaggaggc gggcgacggt
      901 ggtcttgccg gtgcccgggt tgccggtgaa cacgaggtgg tggctggtct cgatcgtggg
      961 caggccgcgc tcggcgcgca gggcctggat ctgcaacagg ctggtcagct tggcgatctc
     1021 gttcttcacc gtgtcgaggc cgaccagttg atcgagctcg gccatcagat cggtgatcgt
     1081 gcgcatcggc gccagcgcag cttcggcggc ggtcgccgcg gggtcgactg ccgttgcggt
     1141 tgcggcggtg aggccacccg cgtccgtccg gcctgtggtt gtgttgttgc cggttgtgtt
     1201 gttgccggtt gtgttgttgc cggttgtgtt gtcgatcgtc ggggccggcc tggctgccgg
     1261 gtcgttcggc agcgatcggg ggagtgccga gcgcgccggg acggggcacg cccgctgcgt
     1321 ccatcgcttg cagcatgatc agacggaagc gttcgatgtg gtcgagttcg gtcggcgagg
     1381 cgaccaggtc gagcgacgcg cacagccggg tgaggcgcag cgctcgatcg tggtagcggt
     1441 ggctgcgagt ggtgcggtcg cgggcatcgg cgcgcaccag caggtcgaac agcactgacg
     1501 gcgtcgccgc ccacgccgcc ttgcccgtca gcacgccgct ggcgcgcaat tcggccggcg
     1561 tcccgatcac cgccggttcg ctgatcacgc cgatgccgga tacatacgcc cacgactcgt
     1621 cgtcggtgat gcggccgtcg gcgtcgagcg ccgcagccac gatcgccgca gcttccacga
     1681 cgacgtcggc gcgatgggac ccgacgctgc gacccgacag ctcggcgagg tcggctccta
     1741 ccgtgtcgat gaagcgatcg atgtcgtgtc cgagcggccc gcgatccacg tcgggcatcg
     1801 taggcggctc acaccgcgac caggtggtgc cgccggttcg agttgaagcg tcacgacgac
     1861 cacccggtag cgtgcgccgt gtatggctcc ccgattcgaa tcagtgcaat ccgtccgcga
     1921 aggccttcgc gacgtcaact atctcgcgga cgaaggcatt gccggcgtcg cctttctggc
     1981 cgatcgcctg ggcaagccgg tgctggtcga ggggccggca ggcaccggta agacccagtt
     2041 ggccaagagc gtcgccgaga tgacgggtgc ccgcctgatc cgcctccagt gctacgaagg
     2101 cctcgacgaa tcgaaggcgc tgtacgagtg gaactacaag aagcagctgc tgcgcatcca
     2161 ggccgacaag aacagcgact cgtggagcac ggtccacgac gacatcttct ccgacgagtt
     2221 cctgctcacc cgcccgctgc tcgaagcgat caccgcggat gatcccgtgg tgttgctgat
     2281 cgacgaggtc gaccgtgtcg aggtcgagac cgaggcgctg ctgctggaga tcctctccga
     2341 gtaccaggtg tcgattccgg agctgggcac gatcacggcc aagcaggtgc cgctggtgtt
     2401 cctcacgtcg aacaacacgc gtgagctcag cgaggcgctg aagcggcgct gcctgttcct
     2461 ccacgtcgac tacccggaca tggagcgcga gaagcagatc gtgctcacca aggtgcccgg
     2521 cgtcagcgag aacctggccg atcagatcgc ccgcatcgtg cgcagcatcc gccaactcga
     2581 cctgaagaag gccccgagcg tcagcgagac catcgattgg gcccgcactc tggtgctgct
     2641 cggcatcgac cacatcgagg cccagcaagc caaggacacc ctccacatcc tcctcaagta
     2701 ccagaccgac atcgccaaag ccgccaaaga gctcagcgtc gaccccaagt aacacccgcc
     2761 cgcgacgtca cgccatccgc gccgtgcccg tcgcgccagc ggtgtggaag tggtcgaaac
     2821 cagcgagccg gtcgagccgt actgcagcac cgtccggcgc ttcggccccg gtgaccgagc
     2881 cgtac
//
LOCUS       DAORNF010000425         2881 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_26214_length_2881_cov_1.634820, whole genome shotgun sequence.
ACCESSION   DAORNF010000425 DAORNF010000000
VERSION     DAORNF010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2881
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26214_length_2881_cov_1.634820"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..532)
                     /locus_tag="PLV68_15145"
     CDS             complement(<1..532)
                     /locus_tag="PLV68_15145"
                     /inference="COORDINATES: protein motif:HMM:NF016207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HRE02636.1"
                     /translation="MYVPSFTAIDDEQARGWLATNSPDGADSPPAATLMPILWRDDTV
                     IAHMAKANPHWRAITDAAPGLFIVSGPEAYISPSRYASKAEHGKVVPTWNYVAVHLIG
                     TVRIHRDPEWVLNAVTMLTDRHERPRAEPWQVSDAPSDYVEALLNAIVGVEMRVTSVD
                     GKAKLSQNRSATDRAGV"
     gene            complement(698..1744)
                     /locus_tag="PLV68_15150"
     CDS             complement(698..1744)
                     /locus_tag="PLV68_15150"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HRE02637.1"
                     /translation="MLTAIADVEALEECVGSRPPGWHLKSIDHLDEHCETLLKAAPFA
                     VVGVVVGDGSVHTTSIGGEPGVVAVSSQRELALPHLANLDAPDGSGVSFLAFVPGYRE
                     TLRINGYLRSGGRVVEVEEAFLHCAKAVIRSHLWDPAPAPTTWLPIAASNTEAGDLTS
                     PHIAAFLAACPLVVVSSRDDQGSADTSPKGDPPGSVIQVLDHRTVAIADRPGNRRTDT
                     MHNLMSCDAISVLALIPGSDHVVEVRGRAIVTDDAAVRAGFSVNDKVPKAAIVVTADH
                     VEMRHEAGLAAASLWDVTRHVDPDSLPKSTRIWVDQVKRNNDRGLAAAAGRKMVNAKM
                     LEIGIAKDYRDNLY"
     assembly_gap    1905..2151
                     /estimated_length=247
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2152..2407
                     /locus_tag="PLV68_15155"
     CDS             <2152..2407
                     /locus_tag="PLV68_15155"
                     /inference="COORDINATES: protein motif:HMM:NF016840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HRE02638.1"
                     /translation="FEADPDWDFTSALDDTLADTVALYEQACARSRDAADGEPLDAMA
                     RNSTRPFNLRWACVHLIEETARHLGQMDILREITDGTTGE"
     gene            2495..>2881
                     /locus_tag="PLV68_15160"
     CDS             2495..>2881
                     /locus_tag="PLV68_15160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02639.1"
                     /translation="MPLGGDHPPRRSVLDAFDDAVVGAAGDDQPVSDAVGSLMVVVMT
                     ALRLGGTERVGQARPRVELHRAVAAPVRVGADVLMQRAAAGDVHHLQASAQTEHRHVD
                     GDRGVAGQAIDALGNGLNSTVTLLEST"
BASE COUNT          402 a          868 c          923 g          441 t
ORIGIN      
        1 tgacgccggc tcggtcggtg gctgagcggt tctggctgag cttggccttg ccgtccaccg
       61 acgtgacgcg catctcgaca ccgacgatcg cgttgaggag cgcttcgacg tagtcgcttg
      121 gcgcatcgga cacctgccac ggctcggccc gaggtcgttc gtgccgatcg gtgagcatgg
      181 tcactgcgtt cagcacccat tccgggtcgc ggtggatgcg aacggtgccg atgaggtgga
      241 cggcgacgta gttccaggtg ggaacgacct tgccgtgctc ggccttggat gcataccgcg
      301 acggcgagat gtacgcctcg ggtccgctga cgatgaacag ccctggcgct gcgtcggtga
      361 tcgcgcgcca gtgcgggttg gccttggcca tgtgggcgat aacggtgtca tcgcgccaga
      421 ggatcggcat gagcgttgcg gcaggcgggc tgtcggcgcc gtccgggctg ttggtggcga
      481 gccaccccct cgcctgctca tcgtcgatgg cggtgaagga cgggacgtac atggtggttc
      541 tgctccgttg gtgacggccc gcaatcgctg gtgagatcac ggtaaaccct gacgcccgct
      601 ggttgcgcgc ctgttcgtcg agcctgccgg gcacgcgtca ggatttcgac gaggccgtcg
      661 atcacgaacc aggcgcgacg ctgtcccgac gtggcgatca gtagaggttg tcgcggtagt
      721 ccttggcgat gccgatctcg agcatcttgg cgttgaccat cttgcggcct gccgcggcag
      781 cgagcccgcg gtcgttgttg cgcttgacct gatcgaccca gatccgggtc gacttcggca
      841 acgagtccgg gtcgacgtgg cgtgtgacgt cccacagcga ggccgcggcg agaccggctt
      901 cgtgccgcat ttcgacgtgg tcggcggtga cgacgatcgc ggccttcggc accttgtcgt
      961 tgacggagaa gccggcccgg accgccgcgt cgtcggtgac gatcgcccgc ccgcgcactt
     1021 cgacgacgtg atcggagccg gggatgagcg cgagcacgct gatcgcgtcg cagctcatca
     1081 ggttgtgcat cgtgtcggtc ctccggttgc ccggccggtc ggcgatcgcc acggtgcggt
     1141 gatcgagcac ctggatgacc gagcccggcg ggtcaccctt cgggctggtg tcggccgagc
     1201 cctgatcgtc tcgcgacgac accacgacca gcgggcacgc ggccaggaag gcggcgatgt
     1261 gcggtgaggt cagatcgccc gcttcggtgt tggacgcagc gatggggagc caggtggtgg
     1321 gcgcaggggc agggtcccac agatgtgacc ggatcaccgc cttggcgcag tgcaggaatg
     1381 cctcttcgac ttcgacaaca cggccgccgg agcgcaggta cccgttgatc cgcagcgttt
     1441 cgcgatagcc gggcacgaac gccaagaagc tgaccccgga gccgtccggg gcgtcgaggt
     1501 tggcgaggtg cggtagcgcc agttcccgtt gtgacgagac cgcgacgacg ccgggctcgc
     1561 cgccgatgga ggtggtgtgc accgacccgt cgcccacgac gacgccgacg accgcgaacg
     1621 gcgcggcctt gagcagcgtc tcgcagtgct cgtcaagatg gtcgatcgac ttgaggtgcc
     1681 accccggtgg acgtgacccg acgcactcct ccaacgcttc gacatctgct atcgctgtca
     1741 acatctccgc accccctggc cctgagacca cctgtcaagc caccttaaca tgctgcaggc
     1801 cccccgcgga aagcggttcg ccgggggagc gccttggtgg gatgatctcg cgcatgtccg
     1861 agacaccggt acccggcacc acccccagcg gggagcgctc cttcnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncttcgaggc
     2161 cgaccccgac tgggacttca cgtcggccct ggacgacacg ctcgccgaca cggtcgcgct
     2221 gtacgagcag gcctgtgccc gcagccgtga cgcggccgac ggcgaacccc tcgacgcgat
     2281 ggcgcgcaac agcacccgac cgttcaacct gcgctgggcc tgtgtccacc tcatcgaaga
     2341 gaccgcccgc catctgggcc agatggacat cctccgcgag atcaccgacg gcaccaccgg
     2401 cgagtgaggt cgtagagcaa ccgctcagcg gggcgttgac gaaggtgtcg aagagtcgtt
     2461 gttggatggg gtcgtcggcg gcatcgtctt cggggtgcca ctgggtggcg accacccacc
     2521 gcgacgcagc gtgctcgatg ccttcgacga tgccgtcgtc ggcgcggccg gtgacgacca
     2581 gcccgtctcc gatgcggtcg gcagcctgat ggtggtagtg atgacagcgc tgcggctcgg
     2641 tggtaccgaa cgcgtcggcc aggcgcgacc ccgcgtcgag ttgcaccggg cggtggcagc
     2701 gccagtgcga gtcggtgccg atgtcctgat gcagcgtgcc gccgcaggcg acgttcatca
     2761 cctgcaggcc tcggcacaga ccgagcaccg gcatgtcgac ggcgatcgcg gcgtcgccgg
     2821 gcaggcaatc gacgccctcg gcaatggcct caactcaacc gtcaccttgc ttgagagcac
     2881 c
//
LOCUS       DAORNF010000426         2877 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_26252_length_2877_cov_1.728916, whole genome shotgun sequence.
ACCESSION   DAORNF010000426 DAORNF010000000
VERSION     DAORNF010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2877
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26252_length_2877_cov_1.728916"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="PLV68_15165"
     CDS             complement(<1..209)
                     /locus_tag="PLV68_15165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02640.1"
                     /translation="MAKLGTWHGDTLQTAAWAEQAADAVTPVDRRREVHLRLDAATTL
                     LNAGHLDRAEVAGRRALTASRECPDL"
     assembly_gap    264..381
                     /estimated_length=118
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<382..561)
                     /locus_tag="PLV68_15170"
     CDS             complement(<382..561)
                     /locus_tag="PLV68_15170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02641.1"
                     /translation="MSGLPKAVDDPGIEVAGPAEPDDPVTVAGVIAEAALTPLLLAAN
                     GDPAAAWNTWLALRAS"
     gene            complement(629..1246)
                     /locus_tag="PLV68_15175"
     CDS             complement(629..1246)
                     /locus_tag="PLV68_15175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02642.1"
                     /translation="MRIERWLGGAAAILVLAACGGSSDDNSAGNRQPVVTEQGSRTDA
                     GENASPDADIDADADIDADASSSGDGVIGASTDMCALFSVEDATAAAGTDVELAAEQL
                     PHSCVYVPVDPTKAVFVNVAYVADGLVDASIEQVAALTLGYGSPGETQGGIEPVGSID
                     GAVAIDAFGVDTVMMPVGDDIVTVSVLGGARAATVGLAEVVSANL"
     assembly_gap    1407..1525
                     /estimated_length=119
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1526..>1887
                     /locus_tag="PLV68_15180"
     CDS             <1526..>1887
                     /locus_tag="PLV68_15180"
                     /inference="COORDINATES: protein motif:HMM:NF037979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02643.1"
                     /translation="VGIGSIWKFPYEVGANGGGAFVLVYLVGLALVVTPMMLAEFAIG
                     RRGRADAATSIEVTAVSESASPRWRTVGVFGAVTSFLILSFYAVIGGWAVAYAGDTLI
                     HGLPQGTAAVNDQFDDLL"
     assembly_gap    1888..1897
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1898..2824
                     /locus_tag="PLV68_15185"
     CDS             <1898..2824
                     /locus_tag="PLV68_15185"
                     /inference="COORDINATES: protein motif:HMM:NF012435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HRE02644.1"
                     /translation="RMVALHAIFLAAVALVVVRGVQRGIETSMKILMPLLAVLLIALA
                     AFSMSTGDADAAMRFLFVPDLDRLTGRGVLDALGLGFFSIGVGLGILLTYAAYSPPSI
                     GLKTAAVVSVAGDTAVSLLAGLAIFPVVFANDVDPASGPGLAFVSLPLAFDAMPGGRW
                     AASAFFVMLAVAALGSAVSMLEAVVAVLDRRFGWSRIRGTLVVASACFIAGLATVLSF
                     NRWAGFHPLSSVGRYAKATIFDLLDDLTSHVLLPLGGVALAVFAGWVMPARFLGDELR
                     LRGVPRAGLRFTLRFVAPVLVIAATLASLISA"
BASE COUNT          327 a          949 c          887 g          467 t
ORIGIN      
        1 agatcggggc actcgcggct ggcggtgagg gcccgtcgcc ccgccacctc cgcgcggtcg
       61 aggtggccgg cgttgagcaa ggtcgtggcc gcgtcgaggc ggaggtgcac ctcgcggcga
      121 cggtccaccg gcgtgaccgc gtcggccgcc tgctcggccc acgctgcggt ctggagggtg
      181 tcgccgtgcc aggtacccaa cttggccacc gcccgaccag cgtcggcgcg atcgagcgga
      241 tcgctcgctg ctgcctcggc ccgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn ngctggcacg cagcgcgagc cacgtgttcc acgcggctgc
      421 ggggtcgccg ttggctgcca acagcagcgg ggtgagagcg gcctcggcga tcactccagc
      481 gaccgtgact gggtcgtccg gttcagctgg cccggctacc tcgattccgg gatcgtcgac
      541 cgccttcggc agcccgctca tggacccgat catcgcgcga cggccggcga ggccgcgaac
      601 gcgccgcgac gcggttgcgg tgggcgcgct acaggttggc cgagaccacc tcggccaggc
      661 cgaccgttgc ggcgcgagcg ccacccaaca ccgagaccgt cacgatgtcg tcgccgaccg
      721 gcatcatcac agtgtccact ccgaacgcgt cgatggccac ggcaccgtcg atcgacccga
      781 ccggttcgat cccgccctgg gtctcgccgg gcgatccgta gccgagcgtc agcgccgcca
      841 cctgttcgat cgacgcgtcg acgagcccgt ccgcgacata ggcgacgttg acgaacaccg
      901 ccttcgtcgg gtcgacagga acgtacacgc acgagtgggg aagctgctcg gcagccaact
      961 cgacgtcggt accggcggcc gccgtcgcgt cctccacgga gaacaacgca cacatgtccg
     1021 ttgacgcgcc gatgaccccg tccccactgg agctggcgtc ggcgtcgatg tcggcgtcgg
     1081 cgtcgatgtc ggcatcgggc gaggcgttct cgccggcatc ggtgcgggaa ccttgctcgg
     1141 tgacgaccgg ctggcggttg cccgccgagt tgtcatcgct ggatccgcca caggctgcga
     1201 gcaccaggat cgctgctgct ccgcccaacc atcgctcgat gcgcatgccg tcctccgcgt
     1261 ccgggccggg ccggcgcccg actcggtgtg caccataggg accagattcg gacccgcacc
     1321 cggggaaaac cctcgttggt cacgcaaccg ccaccagcgc ggtcggatgc gcgcgtcacg
     1381 acgctcggat cgaaaatcgg cgggtcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnngtcgg catcgggagc atctggaagt tcccgtacga
     1561 ggtcggcgcg aacgggggcg gggcgttcgt ccttgtctac ctcgtcggcc tcgcactggt
     1621 ggtcacgccg atgatgttgg cggagttcgc gatcggtcga cgcggacgcg ccgatgccgc
     1681 gacgagcatc gaggtgactg ccgtcagcga gtcggcctcg ccgcggtggc ggaccgtcgg
     1741 cgtcttcggg gctgtcacct cctttctcat cctcagcttc tacgcggtca tcggtgggtg
     1801 ggctgtggcc tatgccggcg acacgctcat ccacggactc ccccagggca ccgcggccgt
     1861 gaacgaccag ttcgacgatc tcctcagnnn nnnnnnncgg atggtggcgt tgcatgccat
     1921 cttcctcgct gcggtcgcgc tcgtcgtggt ccgcggtgtg cagcgcggga tcgagacgag
     1981 catgaagatc ctgatgccgt tgctcgccgt gctcttgatc gcattggcag cgttctcgat
     2041 gagtaccggg gacgccgatg cagcgatgcg gttcctgttc gtgcctgacc tcgatcggct
     2101 cactgggcgc ggggtgctcg acgcgctcgg tctcggcttc ttctcgatcg gggtcggtct
     2161 cgggatcctg ctcacctacg cggcctacag cccgccgtcg atcgggctca agaccgcagc
     2221 cgttgtgtcc gtcgccggtg acactgcggt gtcgctcctc gccggcctgg cgatcttccc
     2281 ggtggtgttc gccaacgacg tcgaccccgc cagcggtccc ggcctggcgt tcgtgtcgct
     2341 cccgcttgca ttcgacgcga tgcccggcgg gcgatgggcc gccagcgcgt tcttcgtcat
     2401 gctggccgtc gccgcactcg gctcggccgt ctcgatgctc gaagctgtcg tcgccgtgct
     2461 cgatcgccga ttcggctggt ctcgcatccg tggcacgctg gttgtcgcct cggcctgctt
     2521 catcgctggc ctcgccaccg tgctgtcctt caaccgctgg gccggcttcc acccactcag
     2581 cagcgtcggc cgctacgcca aagcgacgat cttcgatctg ctcgacgacc tgacctccca
     2641 cgtactgctt ccgctcggcg gcgtcgcact cgcggtgttt gccgggtggg tcatgcccgc
     2701 ccggtttctc ggcgacgaac tccggctccg gggcgtaccc cgcgccggtc tgcggttcac
     2761 gctccgcttc gttgcgcccg tcctcgtgat cgccgccacc ctggcgtcgc tgatcagcgc
     2821 atgagcctgc gccggtcacg cgctgcggcc gggatcatcg ggctcctcgt cgtctcc
//
LOCUS       DAORNF010000427         2870 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_26326_length_2870_cov_1.785435, whole genome shotgun sequence.
ACCESSION   DAORNF010000427 DAORNF010000000
VERSION     DAORNF010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2870
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26326_length_2870_cov_1.785435"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..576)
                     /gene="rnc"
                     /locus_tag="PLV68_15190"
     CDS             complement(<1..576)
                     /gene="rnc"
                     /locus_tag="PLV68_15190"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HRE02645.1"
                     /translation="MSTVDHAALDRLSARLGHTFADPALLERAMAHRSWCAEAPGHPP
                     SNERLEFLGDAVLGWVVADLAFREFTDLPEGKLTDLRKSVVNATALAEVATVLEIGPC
                     LLLGRGEDADGGRSRSSILSDAFEAVLGAIYVDAGADAAYDFVTRAIGPRLRAAVANL
                     EHLDYKTLLQELTARLFDVAPVYVITDQGPDH"
     gene            complement(600..884)
                     /locus_tag="PLV68_15195"
     CDS             complement(600..884)
                     /locus_tag="PLV68_15195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HRE02646.1"
                     /translation="MAANAEPLDRAAILAIVRDNLAEILERDPASIAEGQSFVDDLEA
                     DSLALIELVEALEEDLGERGVELRIEDEDLEDLKTVRDAVDYVVARVEGN"
     gene            complement(952..1923)
                     /gene="plsX"
                     /locus_tag="PLV68_15200"
     CDS             complement(952..1923)
                     /gene="plsX"
                     /locus_tag="PLV68_15200"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HRE02647.1"
                     /translation="MLPIAIDAMGGDFAPDAIVAGALQAADVGIPVVLVGPEGLGGIG
                     SLPLITASEVIAMDEDPAGGVRRKKDSTLVRAAEAVRDGKASAMISAGNTGATMASAL
                     LRMGRIKGVSRPAIATAIPVPGSTPTVLLDAGANAEVQPEWLVQFAQMGSVYARHRFG
                     IANPRVGLLSIGEEPGKGDSLRKEAFPLLQAAPGINFIGNVEGRDIMSDGVDVVVTDG
                     FTGNVVLKTLEGSLKAVIKSLLAAFGADPANKPHADALLPSLLPLYEVLDPENHGGAI
                     LLGVDGVCIISHGSTTAKAMLNAAKVAQEMVQGGMVEQIRAAIAANA"
     gene            complement(1972..2151)
                     /gene="rpmF"
                     /locus_tag="PLV68_15205"
     CDS             complement(1972..2151)
                     /gene="rpmF"
                     /locus_tag="PLV68_15205"
                     /inference="COORDINATES: protein motif:HMM:NF013908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HRE02648.1"
                     /translation="MAVPKKKMSKMKTASRRAAAWKVAAPARSTCPRCGNAKLPHTVC
                     PSCGWYKNRVVIDVG"
     gene            complement(2257..>2870)
                     /locus_tag="PLV68_15210"
     CDS             complement(2257..>2870)
                     /locus_tag="PLV68_15210"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HRE02649.1"
                     /translation="CRSGRIAARFRSTRKTRPRASSTKTAADPAVAGRPFVVNANELL
                     RRPGSERTIDTTLTAAELGLTDERFAPDDPVHAVLHLESTNDGVVVSGSIEVPWHGHC
                     RRCLIQLDRIAVANVDELYQQPVTDPDAFEIVGEQLDLSAMVRELALLDAPHDPLCRH
                     DCAGLCRQCGIDRNNATCECVADAGPSPWDALDALREQLGDAD"
BASE COUNT          436 a          965 c          998 g          471 t
ORIGIN      
        1 atgatcgggc ccttggtcgg tgatgacgta caccggcgcc acgtcgaaca ggcgagccgt
       61 caactcctgc agcagggtct tgtagtcgag gtgctccagg ttggccaccg cggcgcgcag
      121 ccggggtccg atcgcccggg tgacgaagtc gtaggccgcg tcggcaccgg catcgacgta
      181 gatcgccccg agcaccgcct cgaacgcatc gctgagaatc gacgagcgcg accggccgcc
      241 gtcggcgtct tccccccggc ccagcagcag gcacggcccg atctccagca cggtggccac
      301 ctcggcgaga gccgtggcat tgacgacgct cttgcgcaga tcggtcagct tgccttcggg
      361 caggtcggtg aactcgcgaa aggcgaggtc ggccacgacc cacccgagca ctgcatcgcc
      421 caagaactcc aggcgctcgt tggatggggg gtgtccgggc gcttcggcac accacgagcg
      481 atgcgccatc gcccgttcca gcagcgccgg gtcggcgaag gtgtgcccga gccgggcgct
      541 gagccgatcg agcgcggcgt gatcgaccgt gctgatcttg gcgcggcgag cagccagggt
      601 cagttcccct cgacgcgggc gaccacgtag tcgaccgcgt cgcgcacggt cttgaggtct
      661 tccaggtcct cgtcctcgat gcgcagttcg acgccgcgct cgccgaggtc ctcttcgagc
      721 gcttcgacca gttcgatcag cgccagcgag tcggcctcca agtcgtcgac gaacgattgg
      781 ccttcggcga tcgaggcggg atcgcgctcg aggatctcgg ccaggttgtc acggacgatg
      841 gccaggatcg cggcccgatc gagtggttcg gcattggcgg ccacagcggc tccaatcttc
      901 gtagtgtgag gcgggtgcag gtagggcggt cggcggtggc agcgagccac cctatgcgtt
      961 ggctgcgatg gcagcacgga tttgttcgac catgccgccc tgcaccatct cttgcgcgac
     1021 cttggccgcg ttgagcatcg ccttggcggt ggtggaaccg tgcgagatga tgcacacgcc
     1081 gtcgacgccg agcaggatcg caccgccgtg gttctcgggg tcgagcacct cgtacagcgg
     1141 tagcaggctg ggcaacagcg cgtcggcgtg aggcttgttg gccggatcgg ccccgaaggc
     1201 ggccagcagc gacttgatca cggctttgag gctgccttcg agcgtcttca acacgacgtt
     1261 gccggtgaac ccgtcggtga ccacgacgtc gacaccgtcg gacatgatgt cacgcccttc
     1321 gacgttgccg atgaagttga tgcctggcgc cgcctgcaac agcgggaacg cctccttgcg
     1381 cagcgagtcg cctttgcccg gctcttcacc gatggacaac aggccgaccc gcgggttggc
     1441 gatgccgaag cgatgccggg cgtagacgct gcccatctgc gcgaactgga ccagccactc
     1501 gggctgtacc tcggcattgg cgccggcgtc gagcagcacg gtgggcgtcg atcccggcac
     1561 cgggatggcg gtggcgatgg ccgggcggct gacacccttg atgcgcccca tccgcagcag
     1621 cgccgaggcc atggtggcgc cggtgttgcc ggccgagatc atggcgctgg ccttgccgtc
     1681 gcgcaccgcc tcggccgcgc gcacgagggt ggagtccttc ttgcggcgca ccccaccggc
     1741 cgggtcctcg tccatggcga tgacctcgct ggccgtgatc agcggtagcg agccgatgcc
     1801 gccaaggcct tccggcccga cgagcacgac gggaatgccc acatcggctg cttggagcgc
     1861 ccccgcgacg atcgcgtcag gggcgaagtc gccacccatc gcgtcgatgg cgatcggcag
     1921 catctgcgta tcgatggtga cctggcttca gccggggatc agacggctgg atcagccgac
     1981 gtcgatcacg acgcggttct tgtaccaacc gcacgacggg cacaccgtgt gagggagctt
     2041 cgcgtttccg cagcgcggac aggtgctgcg cgcaggcgcc gccaccttcc acgcagcagc
     2101 gcggcggctg gcggtcttca tcttcgacat cttcttctta ggaacagcca ccgtaggtct
     2161 ccagcgtggt caatcgaccg ctgcagggta gcgccgattc accgcgatcg cgccgccaca
     2221 acccgccgcg ctcgcccgca gcgctgctga tggtgttcag tcggcatcgc cgagttgctc
     2281 gcgcagcgcg tcgagcgcat cccacggcga cggccccgcg tcggcgacgc actcgcaggt
     2341 ggcgttgttg cggtcgatgc cgcactgccg gcacaacccg gcgcagtcgt gccggcagag
     2401 agggtcgtgc ggggcgtcga gcaacgccag ctcgcgcacc atcgccgata ggtcgagttg
     2461 ctcgccgacg atctcgaacg cgtcgggatc ggtgacgggt tgctggtaca actcgtcgac
     2521 gttggcgacc gcgatccgat cgagctggat caggcaccgc cgacagtggc cgtgccacgg
     2581 cacctcgatc gaaccgctca ccacgacccc gtcgttggtg ctctccaggt gcaggacggc
     2641 gtggaccggg tcgtcggggg cgaagcgctc atcggtgagg cccagttcgg ccgcggtgag
     2701 tgtggtgtcg atcgtgcgct cggaacctgg ccggcggagc agttcgttgg cgttgacgac
     2761 gaaggggcga ccagccacgg ccgggtcagc cgcggtcttg gtcgaagaag cccttggtcg
     2821 ggtcttcctc gtcgatcgga acctcgctgc gatgcgcccc gatcgacagc
//
LOCUS       DAORNF010000428         2866 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_26367_length_2866_cov_1.829954, whole genome shotgun sequence.
ACCESSION   DAORNF010000428 DAORNF010000000
VERSION     DAORNF010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2866
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26367_length_2866_cov_1.829954"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="PLV68_15215"
     CDS             complement(<1..188)
                     /locus_tag="PLV68_15215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02650.1"
                     /translation="MVWKLRATIGHGWLREHYGRAPIITADHREAAQYATRAAVEADL
                     ALLTPTPVALEPIEVEDGP"
     gene            314..628
                     /locus_tag="PLV68_15220"
     CDS             314..628
                     /locus_tag="PLV68_15220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02651.1"
                     /translation="MSTTHTTTEATTTAAEIAAKVAEGIRTGAFDADDMHSLGTDDGL
                     IVALNPYRHAAWAGIGLDRKHFDDWANGATFVAWTTDNGVMVWAGMDLDPDLMHTAEV
                     AL"
     gene            625..>1147
                     /locus_tag="PLV68_15225"
     CDS             625..>1147
                     /locus_tag="PLV68_15225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02652.1"
                     /translation="MTTTEIATIDFDVTAWFAEFIDEYDIAGVQADVLAAINALAPGS
                     AVVAGHGWEGVVYCDVDDADEIGETDWQALIDSIDLDAIAERHHIATNAALHQITDAV
                     HVALTALAPFAGAALRVHPSEIRARCTSIVAELQSGDDRLAAEACLSVMGLLWPAAAP
                     EDCGHAAWWTSPLG"
     assembly_gap    1148..1181
                     /estimated_length=34
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1332..>2181
                     /locus_tag="PLV68_15230"
     CDS             1332..>2181
                     /locus_tag="PLV68_15230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02653.1"
                     /translation="MSVPWWRPSGPTAALDLVLTLIPQAERALIRAAHFAMPDLYPPT
                     AALDPELRWMADDLAQSLGGWLVWVSRDATAMIRQAARTIPPPDGAIASGVIPQPRGT
                     FWFDADPLEVADVSEASPTVPLVGLTWTAAYEHITVDDPLRVAAVTVFPWAAGPEPFV
                     GSPSTVPLAEDAARMIPTGSGVTISRDLAAVWDHPWADTIKLALAAWVMCGQILPQHI
                     ISATRAQRRRAGRLTTPPAPGWGDVHVTTLRRHAQGSDTTTPDDDEARYHHQWIVSGH
                     WRWQPCG"
     assembly_gap    2182..2200
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2297..>2608
                     /locus_tag="PLV68_15235"
     CDS             2297..>2608
                     /locus_tag="PLV68_15235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02654.1"
                     /translation="MMTTIDELADNWLVGYPPRTATSYRTAYRRWLTWCVCNDLAPLT
                     VTRVDIQRWIASLTDEGLKPRTVTSYATAIAGLYRSAYLDGHIDRDPAAHMRRPRTPT
                     RS"
     assembly_gap    2609..2621
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2622..>2866
                     /locus_tag="PLV68_15240"
     CDS             <2622..>2866
                     /locus_tag="PLV68_15240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02655.1"
                     /translation="ILMLDAAERRTDPTPAALLHLLALNGSRIGELLALDVTDIVTRH
                     RWRIMRMATRKDGSPNDFSLAARTATAVDRAIDGRTI"
BASE COUNT          476 a         1094 c          838 g          392 t
ORIGIN      
        1 ggcccgtcct cgacttcgat tggctctaga gcgacgggcg ttggggtgag gagcgcgagg
       61 tcagcctcga ctgcagccct cgtcgcgtac tgcgcggctt cgcggtggtc ggcggtgatg
      121 atcggggcgc ggccgtagtg ctcgcggagc cagccgtggc cgatggtggc gcggagcttc
      181 caaaccatcg actcagtgtc gcgcatcgcg gcagtgggtc gtcaccggca gaaccgagcc
      241 aacctcggga aatctcggaa atgtcaagcg atatcgcttg acaacctcgg tctgtcatgc
      301 aatactgtat gacatgagca cgacgcacac caccaccgaa gccaccacca ccgctgcgga
      361 gatcgcggcc aaggttgccg agggcatccg caccggcgct ttcgacgccg acgacatgca
      421 cagcctcggc accgacgacg gcctgatcgt cgctctcaac ccctaccgcc acgccgcctg
      481 ggcgggcatc ggcctggacc gcaagcactt cgacgactgg gccaacggcg caacgttcgt
      541 ggcctggacc accgacaacg gcgtcatggt gtgggccggc atggacctcg accccgacct
      601 gatgcacacc gctgaggtgg cgctgtgaca acgactgaga tcgccaccat cgacttcgac
      661 gtgaccgcct ggttcgccga gttcatcgac gagtacgaca tcgccggcgt gcaagccgac
      721 gtcctggcgg cgatcaacgc gctcgcccca ggatcggccg tcgtcgccgg acacgggtgg
      781 gaaggcgtcg tctactgcga cgtcgacgac gccgacgaga tcggagagac cgactggcag
      841 gcactgatcg actcgatcga ccttgacgcg atcgctgagc ggcaccacat cgcgaccaac
      901 gccgccctgc accagatcac cgacgccgtc cacgtcgcgc tgaccgcgct cgccccgttc
      961 gccggcgccg cgctgcgggt ccacccgtcc gaaatccggg cacggtgcac cagcattgtt
     1021 gccgagctcc aatccggtga cgaccgcctc gccgccgaag cctgcctcag cgtgatgggt
     1081 ctgctgtggc ccgccgctgc gccagaggac tgcggacacg ccgcgtggtg gacgagcccg
     1141 ctcggacnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncgacgacac cgacgcggtc
     1201 acgcactcgg cagccgcagc gatgttgggg gtgacgagag ggacggtcgg ccagcttgtg
     1261 caccgcggca ccctcgcccg gcatcctgac gggggcgtga gccgcgccag tgtgctcgcc
     1321 cggctcgccc gatgagcgtc ccgtggtggc gcccgtccgg gcccaccgcc gcgctcgacc
     1381 ttgtgctcac cctgatcccg caagccgagc gggctctcat ccgggccgcc cacttcgcga
     1441 tgcccgacct ttacccgcca actgcagccc tggacccgga gttgcggtgg atggccgacg
     1501 atctcgccca gtcgctcggc ggctggctgg tgtgggtctc acgggacgcg acagcgatga
     1561 tccgtcaagc agcccgcaca atcccaccac cggacggggc gatcgcctct ggggtgatcc
     1621 cgcaaccccg cggcaccttc tggttcgacg ctgatcccct cgaggtcgct gacgtgtccg
     1681 aggcgtcacc aacggtcccg ctcgtcgggc tcacctggac cgccgcgtac gagcacatca
     1741 ccgtcgatga cccgctgcgc gttgccgcag tgaccgtgtt cccgtgggcc gcgggaccgg
     1801 aaccgttcgt agggtcaccc tccaccgtcc cgctcgccga agacgccgca cgaatgatcc
     1861 ccaccgggtc cggagtcacg atcagccgcg atctcgccgc cgtctgggat cacccgtggg
     1921 ccgacacgat caaactcgcg ctcgcggcgt gggtgatgtg cggccaaata ctgccgcaac
     1981 acatcatctc agcgacacgc gcgcaacgcc gccgcgccgg tcgactcacc acaccaccag
     2041 cacccggctg gggcgacgtc cacgtcacca ccctgcgccg ccacgcccaa ggcagcgaca
     2101 ccacgacccc tgacgacgac gaggcccgct accaccacca gtggatcgtg tccgggcact
     2161 ggcggtggca accctgcgga cnnnnnnnnn nnnnnnnnnn ccgcgcgcac cgtcgcctca
     2221 cctggatacc cccgcacatc aaaggccccg acggagcccc gttgctccag atcgcccacg
     2281 tcgccaagct ggtccgatga tgaccacgat cgacgagctc gccgacaact ggctcgtcgg
     2341 ctacccgccg cgcaccgcca ccagctatcg gaccgcgtac cgacgctggc tcacctggtg
     2401 cgtctgcaac gacctcgcac ccctcaccgt cacccgcgtc gacatccaac gctggatcgc
     2461 atcgctcacc gacgaaggcc tcaaaccgcg gaccgtcacc agctacgcga cagccatcgc
     2521 cggtctctac cgcagcgcct acctcgacgg acacatcgac cgcgaccccg ccgcccacat
     2581 gcgtcggcca cgcaccccaa cgcgatccnn nnnnnnnnnn ncatcctcat gctcgacgcc
     2641 gccgaaagac gcaccgaccc cacccccgcc gcgctgctcc acctcctcgc gctcaacggc
     2701 tcccgcatcg gcgaactcct cgccctcgac gtcaccgaca tcgtcacccg ccaccggtgg
     2761 cgaatcatgc ggatggccac ccgcaaagac ggctcaccca acgacttctc actcgccgcc
     2821 cgcaccgcca ccgcggttga ccgcgctatc gacggccgca ccatcg
//
LOCUS       DAORNF010000429         2861 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_26420_length_2861_cov_2.128653, whole genome shotgun sequence.
ACCESSION   DAORNF010000429 DAORNF010000000
VERSION     DAORNF010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2861
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26420_length_2861_cov_2.128653"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            22..>271
                     /locus_tag="PLV68_15245"
     CDS             22..>271
                     /locus_tag="PLV68_15245"
                     /inference="COORDINATES: protein motif:HMM:NF012874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02656.1"
                     /translation="MAFSVVTNNNALAALQSLNQTQHSMSTTQSRITTGLKVAGAADN
                     ASTFSIAQGMRGDIAGLKSASQSIALGKSTVGVALSAAE"
     assembly_gap    272..315
                     /estimated_length=44
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <316..1178
                     /locus_tag="PLV68_15250"
     CDS             <316..1178
                     /locus_tag="PLV68_15250"
                     /inference="COORDINATES: protein motif:HMM:NF012903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HRE02657.1"
                     /translation="VNVDRAKIQQDMNSIVQSISDIGTAAQFNGVNLLNNSSPAGLTV
                     LTGLNRTSPTAETIGSYTASAQDLRTTTLAINSLNVTSGGSATFTPGATLAVADGNTI
                     TLTAGGIAHTFEFTDGSAALTTTDTATVTAHAVLFVAGDTTQQRLSKAFTAMRDAGFT
                     VNTNEDGSFAIDHQQLTASATSVTGLTAGAVSNPSSVAFNAVNSAITTVNSAVAALGT
                     ASNSLDAAGKFNESLTNALTTGVGQLVDADLAEESANLQALQTRQQLGIQALSIANQQ
                     SGSILSLFRG"
     gene            1796..>2861
                     /locus_tag="PLV68_15255"
     CDS             1796..>2861
                     /locus_tag="PLV68_15255"
                     /inference="COORDINATES: protein motif:HMM:NF012874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HRE02658.1"
                     /translation="MQSLNQTQHSMSSAQSRITTGLKVAGAADNASTFTIAQGMRGDI
                     AGLKAASQSIALGKSTVGVALSAAEQISSKLNDLKAKVTEAQNVNVDRAKIQQDMNSI
                     VQSISDIGTAAQFNGINLLNNSSPAGLDVLTGLNRTSPTAETIATYNAAAQDLRTSTL
                     AINNLNITSSGLATFTPGATLAVADGNTITLTAGGIAHTFEFTDGSASLTTNNTAAIT
                     AYGVLFVAGDTTQQRLAKAFTVIRDAGFTVNTNEDGSFAISHQQLTASATSVTGLTAG
                     AVASSSVAAFNAVNTAITTVNSSVASLGTASNSLDAAAKFNESLTTALTTGVGQLVDA
                     DLAEESANLQALQTRQQLGIQ"
BASE COUNT          524 a         1002 c          846 g          445 t
ORIGIN      
        1 agacacagaa ggcatacgac catggctttt tccgtcgtca ccaacaacaa cgcgctggcc
       61 gccctgcagt cgttgaacca gacgcagcac tcgatgtcga ccacccagag ccggatcacc
      121 accggcctga aggttgcggg tgcggccgac aacgcgtcca ccttctcgat cgcgcagggg
      181 atgcgcggcg acatcgccgg cctgaagtcg gcctcgcaga gcatcgcgct cggcaagtcg
      241 acggtcggcg tcgcactcag cgccgccgag gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnacgtc aacgtcgacc gcgccaagat tcagcaggac atgaacagca
      361 tcgtgcagag catcagtgac atcggcacgg cggcacagtt caacggcgtc aacttgctga
      421 acaactcgtc gccggccggc ctgaccgtgc tgactggcct taaccggacc tctccgaccg
      481 ccgagacgat cggctcctac accgcgtcgg cgcaggatct ccggaccacc acgctcgcca
      541 tcaacagcct taacgtgacc tcgggcggta gcgcgacctt cacccccggt gcgaccctcg
      601 cggtcgccga cggcaacacg atcaccctga ccgccggtgg tatcgcgcat accttcgagt
      661 tcaccgatgg ctcggcggcg ctcaccacca ccgacaccgc caccgtcacc gcgcacgctg
      721 tgctgttcgt cgccggtgac accacgcagc agcgcctctc caaggcgttc acggcaatgc
      781 gcgatgcagg ctttaccgtg aacacgaacg aagacggctc gtttgccatc gaccatcagc
      841 agttgaccgc ttccgcgact tcggtaaccg gccttaccgc cggcgcggtg tcgaatccct
      901 cgtccgtcgc gttcaatgcg gtgaattcgg caatcaccac ggtcaactcg gcggttgctg
      961 cgctcggtac ggccagcaac agcctcgatg ccgcggggaa attcaacgag tctctgacca
     1021 atgccctgac caccggcgtg ggccagctgg tcgatgccga cctcgccgag gaaagcgcca
     1081 acctgcaggc gttgcagacc cgccagcagc tgggcatcca ggcgctcagc atcgccaacc
     1141 agcagtccgg ctcgatcctg tcgctgttcc gcggctaggc tcgatcccca ccgccgcaag
     1201 acaccgcggc ggctgccggc gagagataag cgagggaggg ctccaggcga ccggggccct
     1261 cccgcccggc cggcacgggc acgtccgaca cgctgttgtt tgccgacgac ccctcgcacc
     1321 ccgtggtcgc ggatccctcg catcccatgc gtcgcggctg tagcccgacc gggcggcggt
     1381 tgatggcggg tggccggcgc cacgattggg cgccacgata gaaggcgcca ttgctgtcgg
     1441 cggcgggctt cgatccgttg ccgcgaggcc ggcgcttcgg ttgcggagag cgcgcgttcg
     1501 cgcggctgct cctgcgtgtt cgaggtcgtt gccgcgcgat tatgcccgcg tgctaacgcc
     1561 gtatttaccc atttccgtgt atcgcaagat catcgctggc cccgacacgt gtgcggctcc
     1621 ggcgaaggca aggttccctg tcgggaaggt tgcatgtgct ccgcgctccg cgcgtgctct
     1681 gtgatcgccg acagcatcgc tggccccccc ggcggaaccc gtcgcatcgt cgagacacag
     1741 agggcatacg accatggctt tttccgtcgt caccaacaac aacgcactgg ctgcgttgca
     1801 gtcgctcaac cagacccagc attcgatgtc gtcggcacag agccggatca ccaccggttt
     1861 gaaggtcgcc ggggcagccg acaatgcgtc aacgttcacg attgcgcagg gaatgcgcgg
     1921 cgacatcgcc gggctgaagg ccgcctcgca gagcatcgcg ctcggcaagt cgacggtcgg
     1981 tgtcgcactc agcgccgccg agcagatctc cagcaagctg aacgacctga aggcgaaggt
     2041 caccgaggcg cagaacgtca acgtcgaccg cgccaagatt cagcaggaca tgaacagcat
     2101 cgtccagagc atcagcgaca tcggcacggc ggcacagttc aacggcatca acctgctgaa
     2161 caactcgtcg ccggccggac tggacgtgct gaccgggctg aaccggactt cacccaccgc
     2221 cgagaccatc gccacctata atgctgccgc gcaggatctg cggaccagta cgctcgccat
     2281 caacaatctc aacatcacca gcagcgggct tgcgacgttt acgccgggtg cgacgctcgc
     2341 ggtcgccgac ggcaacacga tcaccctgac cgccggcggc atcgcgcata ccttcgagtt
     2401 taccgatggc tccgcgtcgc tcaccaccaa caacaccgcg gcgatcaccg cgtacggggt
     2461 actgttcgtc gccggcgaca ccacgcagca gcggctggcg aaggcgttca ccgtaattcg
     2521 cgatgccggc ttcaccgtga acaccaacga agacggctcg ttcgccatct cgcatcagca
     2581 gttgaccgct tccgcgacgt cagtgaccgg gctgaccgcg ggtgctgtcg cctcctcctc
     2641 ggtggcagcg ttcaatgccg tcaacaccgc gatcaccacg gtcaactcgt cggttgcctc
     2701 gctcggcact gcgagcaaca gtctggatgc ggcggccaaa ttcaacgagt cgctgaccac
     2761 cgccttgacc accggcgtag gccagctggt cgatgccgac ctcgccgagg aaagcgccaa
     2821 tctgcaggcg ctgcagaccc gccagcagct gggcatccag g
//
LOCUS       DAORNF010000430         2846 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_26594_length_2846_cov_2.017915, whole genome shotgun sequence.
ACCESSION   DAORNF010000430 DAORNF010000000
VERSION     DAORNF010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2846
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26594_length_2846_cov_2.017915"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="PLV68_15260"
     CDS             complement(<1..258)
                     /locus_tag="PLV68_15260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02659.1"
                     /translation="MGMFSFLLRWMASSEDRSPWGDFWFEPVTVRTASGIRVSADNAL
                     RLAAVYASVRILAETMAALPFVLYRQRADGGKDRVTDHWLYR"
     gene            complement(262..573)
                     /locus_tag="PLV68_15265"
     CDS             complement(262..573)
                     /locus_tag="PLV68_15265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02660.1"
                     /translation="MRVDAIDPAESVVLTFDFSPALDAGETLTGTITTLVSMALGSDA
                     TPQNVLNGPPAFGIGNTSVLVGVKGGLVDRDYAIKVVVGTSNPAKVLALVAHLPIREE
                     A"
     gene            complement(563..718)
                     /locus_tag="PLV68_15270"
     CDS             complement(563..718)
                     /locus_tag="PLV68_15270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02661.1"
                     /translation="MLLPLWRCWVLLVVDPRFRVTRSARDWTARKAPRNLTISRMPRH
                     FTVTHAR"
     gene            complement(724..885)
                     /locus_tag="PLV68_15275"
     CDS             complement(724..885)
                     /locus_tag="PLV68_15275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02662.1"
                     /translation="MEIEVFEVAPGQWAYRVGGVYQEWHPDCSGFVPMSQAEATACAT
                     GVAARLSGA"
     gene            complement(896..2362)
                     /locus_tag="PLV68_15280"
     CDS             complement(896..2362)
                     /locus_tag="PLV68_15280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02663.1"
                     /translation="MPTANNLRKLLHRKAWELCTPCPSSTVAGSFLTGDDSGTLPAND
                     GVYGVYSASTVYNYHPDQDAWGQIPASGIAGTFGAGSCGEFLAIGMLGGVLDQTATAG
                     TTTTLTTNRTVVRDLKGCRIRVLSGAGAGYDGTVASNTIGANAIITVTPASATAFDAS
                     SSFRVWSGSLWFWNAGSSAVGFSVYDRATNAWTAKSVTGMPTAWGTHGQLVGTGSAKG
                     PFDTGTSTGGNTSTTLNHSGKSWLANAWTNAQLRITGGTGAGQVRTVTSNTATGLTVA
                     AAWTVTPDATSGYSIEGNDDFLYLLGNASVTLYRYSISGNAWTTLSPATARAAAPGAG
                     FSADWIHGVTDADWSGAQGKLLLQSGTYRQNGRYLYSFRGGASAHLDLYDLAGNTWIA
                     AGTSYGNAQETFSTGSCSVEFAGTIYVMKEATGRIFRFDVARHALEPWTTNVYPVSTA
                     VEGDKLAVVAYRDGATLIPFLYHLVHSRAEMQRMLVIG"
     assembly_gap    2473..2639
                     /estimated_length=167
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          436 a          907 c          891 g          445 t
ORIGIN      
        1 gcggtacagc cagtgatccg tcacccgatc cttgccaccg tccgcccgtt gccggtagag
       61 cacgaagggc aaggccgcca tcgtctcggc caggatgcgc acgctggcat acaccgccgc
      121 caagcgcagc gcgttgtccg ctgacacccg gatgccgctc gcggtgcgca ctgtcaccgg
      181 ttcgaaccag aagtcgcccc acggcgagcg gtcctccgat gacgccatcc agcgcaagag
      241 gaagctgaac atccccatcg ctcacgcctc ctcacgaatc ggcaagtgcg ccaccagcgc
      301 cagcaccttg gccggattgc tcgtcccgac gaccaccttg atggcgtagt cgcgatcgac
      361 cagcccgccc ttgacgccga ccagcaccga cgtgtttccg atgccgaacg ccggcgggcc
      421 gttgagtacg ttctgcggtg tcgcatcgct gccgagggcc atgctgacga gcgtcgtgat
      481 ggtcccggtc agcgtttcgc cggcatcgag cgcgggcgag aagtcgaaag tcaggacgac
      541 cgattcggca ggatcaatgg catcaacgcg catgcgtcac cgtgaagtgc cggggcatcc
      601 ggctgatcgt caggtttctc ggagccttcc tcgccgtcca gtcgcgcgcg ctgcgcgtga
      661 cccgaaatcg cggatcgacg acgagcagga cccagcagcg ccacagaggc agcaacatcg
      721 tggctacgct ccggacaggc gcgcggcaac ccccgtcgcg cacgcggtcg cctccgcctg
      781 gctcatcggt acgaagccgg agcagtccgg gtgccattcc tgatagacac cgcccacgcg
      841 atacgcccat tgccccggcg cgacctcgaa cacttcgatc tccatcggtt gcccctcagc
      901 cgatcaccag catgcgctgc atctccgcgc gcgagtgcac caggtgatac aggaacggga
      961 tcagcgtggc gccgtcccga tacgcgacca cggccaactt gtcgccttcg accgctgtcg
     1021 acaccgggta gacgttggtg gtccacggtt ccagggcgtg tcgcgccacg tcgaaacgga
     1081 agatgcgccc cgtcgcttcc ttcatcacat agatggtgcc ggcgaattcg acgctgcacg
     1141 acccggtgct gaaagtctcc tgcgcgttgc cgtacgacgt gcccgcggca atccaggtgt
     1201 ttcccgcgag gtcatagagg tcgaggtggg cactggcgcc gccgcggaag ctgtagaggt
     1261 agcggccgtt ctgccggtac gtcccggact gcagcagcag cttgccttgc gcgccggacc
     1321 agtcggcatc ggtcacgccg tgaatccagt cggccgagaa gccggctccc ggcgcggccg
     1381 cccgcgccgt tgccggcgac agtgtggtcc aggcattgcc gctgatcgaa taccgataca
     1441 gggtgaccga cgcgttgccc agcagataca gaaagtcgtc gttgccctcg atgctgtagc
     1501 cggaagtggc gtccggcgtg accgtccagg ccgcggcgac cgtcagcccc gtggcggtat
     1561 tgctggttac agtgcgcact tggcccgcac ccgtgccgcc ggtgatgcgc agctgggcgt
     1621 tcgtccaggc gttggcgagc cacgacttgc cgctgtggtt caacgtcgtc gacgtattgc
     1681 cgccggtgct ggtgccggtg tcgaaggggc ccttggcact gccggtgccg accaactggc
     1741 cgtgcgtgcc ccaggccgtc ggcatgcccg tcacgctctt cgcggtccag gcgttcgtcg
     1801 cccggtcata gaccgagaag ccgaccgcgc tactgccggc attccagaac cacagcgagc
     1861 ccgaccacac gcggaagctg ctgctcgcat cgaaagcggt cgcgctggcg ggggtgaccg
     1921 tgatgatcgc gttggcgccg atcgtgttgc tggcgacggt cccgtcgtag cccgccccgg
     1981 caccggacag gacgcgaatc cggcagccct tgaggtcacg gacgacggtg cggttggtcg
     2041 tcagggtcgt ggtcgtgccc gccgtcgccg tctgatccag cactccgccc agcatgccga
     2101 ttgccaggaa ctcgccacag gaccccgcgc cgaaggtgcc ggcgatgccg gaggccggaa
     2161 tttgtcccca ggcatcctga tccgggtggt agttgtagac cgtcgaggcg ctgtagacgc
     2221 cgtagacccc gtcgttcgcg ggcagggttc cgctgtcgtc gccggtgagg aaactgccgg
     2281 ccacggtgct cgacgggcaa ggcgtgcaga gctcccaggc cttgcggtgg agcagtttgc
     2341 gcaggttgtt ggctgtgggc atcggtctag ctcaccagga tctgggtgta gaggcgggcg
     2401 gccgcggtgt ccgagaagtt ccacggctcg aacatccggt agacgagtcg tgcgcccgcg
     2461 ccatcgacca ccnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna
     2641 gcgtggcctg ctgggcgagc aggagcggat cgcccgtgac cggatcgacg ggcacgacgg
     2701 cctgcatgtg cacggtgtcg gccccttcga gtcgggtgaa ggtgtcgacg atgccggcag
     2761 cgttgggaag ttcgatatga ctcatgcggc agatccttta cagcatcacg agttcgtagt
     2821 cggcaccgag caccacggca gtgccc
//
LOCUS       DAORNF010000431         2830 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_26790_length_2830_cov_1.630631, whole genome shotgun sequence.
ACCESSION   DAORNF010000431 DAORNF010000000
VERSION     DAORNF010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26790_length_2830_cov_1.630631"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="PLV68_15285"
     CDS             complement(<1..198)
                     /locus_tag="PLV68_15285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02664.1"
                     /translation="MSYDDQYEDDFDDISDDTDAYSRISQVGDAETLVRRAAAGGAQV
                     ILLQELFATPYFCITEEPRHLA"
     gene            complement(195..>566)
                     /locus_tag="PLV68_15290"
     CDS             complement(195..>566)
                     /locus_tag="PLV68_15290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine adenylyltransferase"
                     /protein_id="HRE02665.1"
                     /translation="MFAIDERLDMVRESLAHVGGVEVAASTGLAIDAARDLGADFMVK
                     GLRNTTDFDIEQQMAHTNQSVGGVATVYLPCRPDQVFVSSRFIREIAQYGRDVSHLVP
                     APVQQRFALRFPPDQPPRDLP"
     assembly_gap    567..659
                     /estimated_length=93
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(760..>1233)
                     /locus_tag="PLV68_15295"
     CDS             complement(760..>1233)
                     /locus_tag="PLV68_15295"
                     /inference="COORDINATES: protein motif:HMM:NF015559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HRE02666.1"
                     /translation="GLSTRPTTDKVRQAVFNSLDSRGVLDGALVVDLFAGSGAMGIEA
                     LSRGAESCVFVENDRAAVAALRANLASLRLGDRARVMVADVMSVAGSLADADIALIDP
                     PYTFDQWPLLLGRVRAGLVVAEGGQELPPIDGWQVVRSKRHGRTVITTLEPSRVA"
     assembly_gap    1234..1271
                     /estimated_length=38
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1310..1528
                     /locus_tag="PLV68_15300"
     CDS             1310..1528
                     /locus_tag="PLV68_15300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02667.1"
                     /translation="MAPHDPGLGNLEPEPEIDCVDCGGRCFLLTHPREDGVWLPGDIV
                     AYRCEDCLDRWDLVLPEPSDARGDTPDS"
     gene            complement(1556..>2520)
                     /locus_tag="PLV68_15305"
     CDS             complement(1556..>2520)
                     /locus_tag="PLV68_15305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HRE02668.1"
                     /translation="QDAINFASLGVVVVDEQHRFGVEQRAALRLKGAGDGDERVVPDV
                     LVMTATPIPRTAAMTVYGDLDVSVLDELPPGRTPITTYWANGGLMELAAWHDVKAAVA
                     EGRQAYVVCPLIDESEKLEVASATEVYERLVHQDLKGLRIGLLHGRLPDAEKEAVMTA
                     FRERRLDVLVATTVIEVGVDVPNATVMVIVDADRFGIAQLHQLRGRVGRGEHASTCWL
                     ITAETEGPSPRIEALVASTDGFELAEVDLDLRGEGTLMDTAQKGRSDLKLASLRRDRE
                     LVGLAREAAFDIVDADPTLAKHPALLDELSLSFDAGQEEFLFKS"
     assembly_gap    2521..2533
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2534..>2830)
                     /locus_tag="PLV68_15310"
     CDS             complement(<2534..>2830)
                     /locus_tag="PLV68_15310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRE02669.1"
                     /translation="LQGDVGAGKTLVAVAALLAAVQGGHQGALMAPTEVLAEQHAAGV
                     GRLLADLRVPDPANLFGDRPLNVKLLTNRITGAERREVLAGLADGSVDIAIGTH"
BASE COUNT          427 a          959 c          878 g          422 t
ORIGIN      
        1 ggcgaggtgg cgcggctctt cggtgatgca gaaatagggg gtggcgaaca gttcctgcaa
       61 caggatcact tgcgcccccc cggcggcggc ccggcgcacc agcgtctcgg cgtcgcccac
      121 ctgactgatg cgcgaatacg cgtcggtgtc atcggagatg tcgtcgaagt cgtcttcgta
      181 ctgatcgtcg tagctcatgg cagatctctc ggtggttggt ccggcggaaa ccgcagggcg
      241 aaacgttgtt gcacgggtgc cggcacaagg tgcgaaacat ccctaccgta ctgggcgatc
      301 tcacggatga agcggctgct cacgaacacc tgatccggcc gacagggcag gtacaccgtg
      361 gcgacgccac cgacggattg gttggtgtga gccatctgct gttcgatgtc gaagtcggtc
      421 gtgttgcgca gccccttcac catgaagtcg gcaccgaggt cgcgggcggc gtcgatggcc
      481 agcccggtcg atgccgcaac ctcgacaccg ccgacgtggg caaggctctc gcgcaccatg
      541 tcgagccgct cgtcgatggc gaacatnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna
      661 atcggatcga agctgcccgg atacagcacc cgccgtgccc cagccgccga cccggttgcc
      721 gctgcaggca tggtgactcc cccgtcgctc attggcgcgt cacgctaccc gcgacggttc
      781 cagcgtcgtg atcacggtgc ggccgtggcg cttggagcgc acgacctgcc agccgtcgat
      841 cgggggtagt tcctggccac cctcggccac gacgagcccg gcccgcaccc ggccgagcag
      901 cagcggccac tggtcgaacg tgtatggcgg gtcgatcagg gcgatgtcgg cgtcggccaa
      961 cgagccggcc acgctcatca cgtcggccac catcacccgc gcccggtcgc ccaaccggag
     1021 cgatgcgagg ttggcccgca acgcagcaac ggcggcgcgg tcgttctcga cgaacacgca
     1081 cgactccgcg ccccgcgaca acgcctcgat acccatcgcc ccgctgcccg cgaacagatc
     1141 gaccaccagt gcaccgtcga gcacgccccg gctgtcgagc gagttgaaca ccgcctggcg
     1201 caccttgtcg gtggtcggcc gggtcgacag gccnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn ncgcccgcta tgacacgcat ccggccagac taggggccga tggcgccgca
     1321 cgatcctgga ctgggcaatc tcgaaccgga acccgagatc gactgcgtcg actgtggtgg
     1381 ccgttgcttc ctgctcaccc atccgcgaga ggacggggtt tggctgcccg gcgacatcgt
     1441 cgcctaccgc tgcgaagact gcctcgaccg gtgggacctc gtccttcccg aacccagcga
     1501 cgcccgcggc gacacccccg actcctgacg cacgctgcac ggctgacgtg ccccgtcagc
     1561 tcttgaagag gaattcctct tggccggcgt cgaagctgag gctcaactcg tcgagcaggg
     1621 cagggtgctt ggccaaggtg ggatcggcgt cgacgatgtc gaacgcggcc tcgcgagcca
     1681 ggccgaccag ttcgcggtca cgccgcagcg acgccaactt cagatcgctg cggcccttct
     1741 gggccgtgtc catcaaggtg ccttcaccgc gcaggtcgag gtcgacctcg gccagctcga
     1801 acccgtcggt cgacgcgacc agcgcttcga tgcgcgggct cggtccttcg gtctccgcgg
     1861 tgatcaacca gcacgtcgac gcgtgttcgc cacgaccgac gcggccgcgg agctgatgca
     1921 actgcgcgat accgaagcgg tcggcgtcga cgatgaccat cacggtcgcg ttgggcacgt
     1981 cgacacccac ctcgatcacc gtcgtcgcga ccagcacgtc gagacgccgt tcacggaacg
     2041 cggtcatcac cgcttccttc tccgcatcgg gcaggcgccc gtgcagcagg ccgatgcgca
     2101 ggcccttcag gtcttggtgg acgagacgct cgtagacctc ggtcgcggac gcgacttcga
     2161 gcttttcgct ctcgtcgatc agcgggcaga ccacgtacgc ctggcgacct tcggcgactg
     2221 cggccttgac gtcgtgccac gccgcgagct ccatcaagcc accgttggcc cagtaggtcg
     2281 tgatcggcgt gcgcccgggt ggcagttcgt cgagcacgct cacgtcgaga tcgccgtaca
     2341 cggtcatcgc cgccgtgcgc gggatcggcg tggcggtcat caccaacacg tcgggcacca
     2401 cccgctcgtc gccgtcaccg gcccccttca accgcagcgc ggcacgctgt tcgacgccga
     2461 agcgatgctg ctcgtcgacc acgaccacac cgaggctggc gaagttgatc gcgtcctgga
     2521 nnnnnnnnnn nnncgtgggt gccgatcgcg atgtcgacgc tgccgtcggc cagaccggcc
     2581 agcacctcgc gccgctcggc gcccgtgatg cggttggtca acagcttgac gttcagcggc
     2641 cggtcaccga acaggttggc cgggtcgggg acgcgcagat cggccagtag gcggccgacc
     2701 ccggcggcat gttgttcggc cagcacttcg gtcggcgcca tcagcgcgcc ctggtggcca
     2761 ccctgcaccg cggcgagcag cgcggccacg gcgaccaacg tcttgcccgc gccgacgtcg
     2821 ccctgcagca
//
LOCUS       DAORNF010000432         2829 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_26798_length_2829_cov_2.045422, whole genome shotgun sequence.
ACCESSION   DAORNF010000432 DAORNF010000000
VERSION     DAORNF010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2829
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26798_length_2829_cov_2.045422"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..796)
                     /gene="nhaA"
                     /locus_tag="PLV68_15315"
     CDS             complement(<1..796)
                     /gene="nhaA"
                     /locus_tag="PLV68_15315"
                     /inference="COORDINATES: protein motif:HMM:NF018648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HRE02670.1"
                     /translation="MTAPQISSAQPTFIQSDTTLARAVRPFVRFSHIEAAGGIVLVAA
                     TVVALVWANSPWKAGYESVWATQIRFEFGSYAFHEDLGHVVNDLLMAVFFFVVGMEIK
                     RELVTGELRDRRAVALPAMAALGGMIVPAAIYLAFNAGSEGVDGWGIPMATDIAFALG
                     VVALLGRRVPTSVKVLLLTLAIVDDIGAIVVIAVFYTDRVEWRFVLAAIACAAVVALM
                     HRGRVVYSPMIAVVGFALWLMVFESGIHATIAGVIMGLLTPARPAQS"
     gene            complement(913..1269)
                     /locus_tag="PLV68_15320"
     CDS             complement(913..1269)
                     /locus_tag="PLV68_15320"
                     /inference="COORDINATES: protein motif:HMM:NF037170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRE02671.1"
                     /translation="MIGIGGVFFKGHDPGRLARWYEDHLGIIAVGESYGDPVWIQQEG
                     PTVFAAFGPEHWDSPHIGSSGWGINFRVSDLDAMVTQLRAAGIAVDVDLEQYPNGRFA
                     QLLDPEGNPVQLWQEM"
     gene            1858..>2829
                     /locus_tag="PLV68_15325"
     CDS             1858..>2829
                     /locus_tag="PLV68_15325"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HRE02672.1"
                     /translation="MTVASRWIPTGRRDELRSLASRSHQVVTLAAITGAITGLVVAGF
                     DRLVVDGVFHHVLNLPAWLMGVLPGVGLLVAFIARRFIGGGASAATADEYLHAFHDPS
                     HRLGWRPFAGRMVAAIATLGSGAPMGLEGPSLYTGATIGSQIQRRSRRSFRDADRRVL
                     LVAGAAAGVSAIFKAPATGAVFALEVPYRGDLARRMLLPALVASAAGYLVFAAINGTD
                     ALFPIEATPGFMFRDLIGVALLGVVAGIGARAFGWLLRRAKRIAVSRHALAATIAAGV
                     TIGGLYALGLALTDDSLVLGSGYRILDWATDPTRSAWLLMAMLAIRCL"
BASE COUNT          468 a          995 c          909 g          457 t
ORIGIN      
        1 cggattgtgc ggggcgggcg ggagtgagca gccccatgat cacgccggcg atggtggcgt
       61 ggatgccgga ctcgaagacc atcaaccaca gcgcgaaccc cacgactgcg atcatcggcg
      121 agtagacgac tcggccacga tgcatcaacg ccacgaccgc tgcgcaggcg atcgcggcga
      181 gcacgaagcg ccactcgaca cgatcggtgt agaagacggc gatcacgacg atcgcgccga
      241 tgtcgtcgac gatcgccaat gtcagcagca gcaccttcac ggacgtgggc acgcggcgac
      301 cgaggagcgc gacgacgccc agggcgaagg cgatgtcggt cgccatcggg atgccccaac
      361 cgtcgactcc ctccgagccc gcgttgaacg cgaggtagat cgcagcgggg acgatcatgc
      421 cgccgagcgc ggccatcgcc ggcagggcga cggcacgacg atcgcgcagc tctccggtga
      481 cgagctcgcg tttgatctcc atcccgacga cgaagaagaa cactgccatc aacaggtcgt
      541 tgacgacgtg gccgagatcc tcgtggaacg cgtacgagcc gaactcgaac cggatctgtg
      601 tcgcccacac ggattcgtag ccagccttcc acggcgagtt cgcccacacg agcgcgacga
      661 cggtggcggc gacgagaacg atgccgccgg cggcctcgat gtgcgagaag cggacgaacg
      721 gtcgcaccgc acgggccagc gtggtgtcgc tctgaataaa ggtcggctgc gcgctcgaga
      781 tctgcggggc tgtcatcggg ggaactcctg agggtcgacg aaatcacgtc gaccagactt
      841 cccgacacaa ccaaggatca ggctaccgct cgtgcacggc cgatctgttg tcccctcggt
      901 gtcggcggtc cgtcacatct cctgccacag ctgcacaggg ttgccttcgg gatcgaggag
      961 ctgtgcgaag cgtccgttcg gatactgctc gagatcgacg tcgacggcga tgccggcggc
     1021 tcggagttgt gtgaccatcg cgtcgagatc ggagacacgg aagttgatgc cccaccctga
     1081 cgatccgata tgtgggctgt cccagtgctc aggaccgaac gcagcgaaca cggtcgggcc
     1141 ctcttgctgg atccataccg gatcgccgta ggactcgccg accgcgatga tgccgaggtg
     1201 gtcctcatac cagcgggcga gccgaccggg atcatggccc ttgaagaaca ctccaccgat
     1261 gccaatcact cgctccaccg ccagagtctg tcacccagcc aacaccacct gccgaacgag
     1321 cgcaccgagc ctggggtcag ggcgtgcatg cactgtgcaa cacttgtaca ctacacatgt
     1381 gcagacgcca gcgccgacac gagcccagac cgtcgatctg accgaggcgt tgctcacggc
     1441 ttcgcgagcg ctcgttgccg tcgctgctcg ctcgttggca agcgctgaca gcgacgtgac
     1501 acttccgcag taccgggcgc tggtcgtcct cgccgctcgc gggccccgcc gcgtcggcga
     1561 gctcgcggag gcactcgaca tccatccatc gactgcgacc cgactgtgcg atcgactggt
     1621 ggagcacggt ctcatccgac gcgcggtcga ccgctcgaac cgacgcgaga caaccctctc
     1681 gctctctgcg caaggccgcg cgatcgtcga tcgcgtcacc gagacgcgcc gggcggagat
     1741 cggctcgatc gtccggcgga tacctcgcga tctccgcgag ccggcggtgg tcgcgctccg
     1801 cgcctttgcc gacgcagccg gggaaccacc gcaagaagcg tggacgttgg gctggacatg
     1861 accgtcgcct cacgctggat ccccaccggt cgacgcgacg agttgcgaag cctggcgtcg
     1921 cgttctcacc aagtggtcac cctcgccgcg atcacgggcg cgatcaccgg cctcgtggtg
     1981 gcaggtttcg accgtctggt cgtcgacggc gtgttccacc acgtgttgaa tctgccggcg
     2041 tggctgatgg gagtgcttcc gggggtcggg ctgcttgtcg cgttcattgc tcgacggttc
     2101 atcggcggtg gtgcgtccgc agctactgcc gacgagtacc tgcacgcgtt ccacgatccg
     2161 tcgcatcggc tcgggtggcg acccttcgct ggccgcatgg tcgccgcgat cgccacactc
     2221 ggatccggcg cgccgatggg tctcgagggt ccatcgctct acaccggcgc gacgatcggg
     2281 tcacagatcc aacgacgatc cagacgatcc ttccgcgacg ccgaccggcg cgtgctgctc
     2341 gtcgccggtg cggccgccgg tgtctcggcg atcttcaaag cgcccgccac cggtgccgtg
     2401 ttcgccctcg aggtccccta ccgaggcgac ctcgcaagac ggatgctgct ccccgctctc
     2461 gttgccagtg ccgccggcta cctcgtgttc gcggcgatca acggcaccga cgccctgttc
     2521 ccgatcgagg ccacaccagg gttcatgttc cgcgatctca tcggcgtcgc gctgctcggc
     2581 gtcgtcgccg gcatcggcgc ccgcgccttc ggatggctgc tccgccgagc gaagcggatc
     2641 gccgtgtcac gccacgcgct cgccgccacg atcgcggccg gcgtcaccat cggcggcctg
     2701 tacgccctcg gtctcgcgct cactgacgac tccctcgtgc tcggctccgg ctaccgcatc
     2761 ctcgactggg cgaccgaccc gacccgctcc gcatggctgc tcatggcgat gctcgccatc
     2821 cgctgcctc
//
LOCUS       DAORNF010000433         2820 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_26905_length_2820_cov_1.568174, whole genome shotgun sequence.
ACCESSION   DAORNF010000433 DAORNF010000000
VERSION     DAORNF010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2820
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26905_length_2820_cov_1.568174"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="PLV68_15330"
     CDS             <1..251
                     /locus_tag="PLV68_15330"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRE02673.1"
                     /translation="VGIGAEAIRDMLKAIDLAIQAHGALGVTDDTVLAYWYRHERAAR
                     IYDGADEVHKTSLGRHLLAAARERAAAPSTSRWTTVPA"
     gene            284..>939
                     /locus_tag="PLV68_15335"
     CDS             284..>939
                     /locus_tag="PLV68_15335"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HRE02674.1"
                     /translation="MNSSAPVGDHTRDLVTGAFGNTGSAIAARLQAGGRRVRTLTSRA
                     QPSRAGTSRAGDDATIEVFGWDDLARAFDGVATFYNTFWMRTGNPGSHGTDYSLAAER
                     SARLIEAAAAAGVERIVHLSVAHADDPAAAKYPYFAAKAGVEAMIRATGITHTIVRPA
                     LIFGGGPGMVEQLAWVLRRMPVMGVAGDGTYRVRPVHVDDVADLVVTAGRFADATATG
                     T"
     assembly_gap    940..949
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <950..1261
                     /locus_tag="PLV68_15340"
     CDS             <950..1261
                     /locus_tag="PLV68_15340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02675.1"
                     /translation="DSSSAEAVVINAVGPDRLTFEELARAVATAIGKRRAFVHLPTAL
                     VIAAGRALGRVAGQELLTGDELRSTIDGLADVDGPATGQVSLTDWLTAHGQHLGRRLD
                     G"
     assembly_gap    1356..1419
                     /estimated_length=64
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1420..>2820)
                     /locus_tag="PLV68_15345"
     CDS             complement(<1420..>2820)
                     /locus_tag="PLV68_15345"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003103909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HRE02676.1"
                     /translation="DNTRPLTTAAGAPVAENDNAMTAGVRGPMLLQDVWFLEKLAHFD
                     REVIPERRMHAKGSGAFGTFTVTNDVTRYTRAAMLSEVGKQTEMFARFSTVAGERGAA
                     DAERDIRGFALRFFTEEGNWDIVGNNTPVFFIRDPHKFADLNRAVKRDPRTNLRSAEN
                     NWDFWTNLPEALHQVTIVMSDRGIPDGYRHMHGFGSHTYSFVNAEGERSWVKFHFVTQ
                     QGIKNLTDAEAAAIVASDRESSQRDLFESIERGDFPRWTLSVQVMPEREASSVPYNPF
                     DLTKVWPHADYPLIEVGVFELNRNPENYFADVEQVAFTPANVVPGVDFSPDRMLQGRL
                     FSYGDAARYRLGVNHHQIPVNYPRGATNVNTYHRDGTMRVDGNQGGVPGYTPNSYGRF
                     EAQPGFDAPALAIEGPGARFNFRDDDDNYYEQPRDLFHLMTPQQQAVLFANTARAING
                     ASEATVERHIANCTKA"
BASE COUNT          424 a          909 c          947 g          466 t
ORIGIN      
        1 ccgtcggcat cggcgccgaa gccatccgcg acatgctcaa ggcgatcgac ctcgccatcc
       61 aggctcatgg tgcgctcggc gtcaccgacg acaccgtgct cgcctactgg taccgccacg
      121 aacgggcggc gcgcatctac gacggtgccg acgaggtgca caagacctcc ctcggccgcc
      181 acctcctggc ggccgcccgc gagcgcgccg cagccccgtc caccagccgc tggacgaccg
      241 tacccgcctg atcgattgcg gcggttcccg ataccgtcgg cccatgaaca gttccgcacc
      301 ggtcggtgac cacacccgcg atctcgtcac gggcgcgttc ggcaacacgg gttcggcgat
      361 cgcggcccgg ctgcaggccg gtgggcgccg ggtgcgcacc ctcacatctc gagctcagcc
      421 gtctcgggcc ggcacctccc gcgccgggga cgacgcgacg atcgaggtct tcgggtggga
      481 cgatctggcc cgggcgttcg atggcgtcgc gaccttctac aacacgttct ggatgcgcac
      541 gggcaacccc ggttcgcacg gcaccgacta ctcgctcgcc gccgagcgtt cggcgcggct
      601 gatcgaggcc gcggccgcgg cgggtgtcga acggatcgtc catctctcgg tcgcccacgc
      661 cgacgatcca gccgcggcga agtatcccta cttcgccgcc aaggccggcg tcgaggcgat
      721 gatccgcgcc accggcatca cgcacacgat cgttcgaccc gcactgatct tcggcggtgg
      781 ccctggcatg gtcgaacaac tcgcgtgggt gttgcgacgg atgccggtga tgggtgtagc
      841 cggtgacggc acctaccgcg tccggccggt gcacgtcgac gacgtggccg atctggtcgt
      901 caccgcaggt cgcttcgccg acgcgacggc gacgggcacn nnnnnnnnng actcgtcatc
      961 agcggaagcg gtggtgatca acgctgttgg gccggatcgg ctgaccttcg aagagttggc
     1021 ccgcgccgtg gccaccgcga tcggcaagcg ccgcgctttc gtccacctgc cgaccgcgct
     1081 ggtgatcgcc gccgggcgcg ccctcgggcg cgtcgccggc caagagttgc tgaccggtga
     1141 cgaacttcgc tcgaccatcg acggtctggc tgacgtggac ggccctgcga ccggccaggt
     1201 cagcctcacc gattggctca ccgcccacgg ccaacacctg ggtagacgac tcgacggctg
     1261 actcgacggc tgactcgacc cgacctgcga cgagtggacc cgttcggcat cgtcaggtcg
     1321 ggtttcggtg gccggcggct ggcggcggaa agcggnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc ggccttggtg cagttggcga
     1441 tgtggcgctc gacagtggct tcggacgcgc cgttgatcgc tcgcgcggtg ttcgcgaaca
     1501 acaccgcctg ctgctgcggc gtcatcagat ggaacaggtc gcggggctgt tcgtagtagt
     1561 tgtcgtcgtc gtcgcggaag ttgaaccggg cgcccggacc ctcgatcgcc aacgccggcg
     1621 cgtcaaagcc gggctgcgcc tcgaagcggc cgtagctgtt gggtgtgtag ccaggaaccc
     1681 caccctggtt tccgtcgacg cgcatcgtgc cgtcgcggtg ataggtgttg acgttggtcg
     1741 caccgcgggg gtagttgacc gggatctggt ggtggttgac gccgaggcga tagcgagccg
     1801 cgtcgccgta ggagaacagt cggccctgca acatgcggtc gggtgagaag tcgacaccag
     1861 gcacgacgtt ggctggggtg aacgccacct gctcgacgtc ggcgaagtag ttctcggggt
     1921 tgcgattgag ctcgaacaca ccgacctcga tcagcgggta gtccgcatgc ggccagacct
     1981 tggtcagatc gaaggggttg tagggcacgg acgacgcttc cctctccggc atcacctgca
     2041 cggacagcgt ccagcgcggg aagtcaccgc gctcgatgct ctcgaacagg tcgcgctgcg
     2101 acgactcgcg gtcgctggcg acgatcgccg cggcctcggc atcggtcagg ttcttgatgc
     2161 cctgctgggt gacgaagtgg aacttgaccc acgaccgctc gccttcggcg ttgacgaacg
     2221 agtacgtgtg tgagccgaac ccgtgcatgt gccggtaccc atcggggatg ccgcggtcgg
     2281 acatcacgat cgtgacctgg tggagtgcct cgggcagatt ggtccagaag tcccaattgt
     2341 tctcggccga gcgcaggttg gtacgcgggt cgcgcttgac cgcccggttg aggtcggcga
     2401 acttgtgtgg gtcgcggatg aagaacaccg gggtgttgtt accgacgatg tcccagttgc
     2461 cctcctcggt gaagaatcgg agggcgaagc cacggatgtc gcgctcggca tcggccgcac
     2521 cgcgctcgcc ggccacggtg gagaagcgag cgaacatctc ggtctgcttg ccgacctcgg
     2581 acagcatcgc tgcccgggtg tagcgggtga cgtcgttggt gaccgtgaag gttccgaatg
     2641 cgccagaacc cttggcgtgc atgcgtcgct cgggaatgac ctcgcgatcg aaatgggcca
     2701 gcttctccag gaaccacacg tcctgtagga gcatgggacc ccgcactcca gccgtcatgg
     2761 cattgtcatt ttcggcgacc ggagccccag cggcggtcgt caacgggcga gtgttatcgg
//
LOCUS       DAORNF010000434         2819 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_26922_length_2819_cov_1.210564, whole genome shotgun sequence.
ACCESSION   DAORNF010000434 DAORNF010000000
VERSION     DAORNF010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2819
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26922_length_2819_cov_1.210564"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>464)
                     /locus_tag="PLV68_15350"
     CDS             complement(<1..>464)
                     /locus_tag="PLV68_15350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02677.1"
                     /translation="AAALALLGMAGLAAGFVATARVTGLPHDYIVRWWWVISAWMWLS
                     IVVSAAALVSRRWQVRVAVGAGVVAAVASVALTVGAVTADPPAPAESEAIIALVDQLD
                     GVLPTDRQYVVEWADRNTWGEVGVGVFVGVNVGVDVAVGVFVACSVGVLLGV"
     assembly_gap    465..488
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<489..>1305)
                     /locus_tag="PLV68_15355"
     CDS             complement(<489..>1305)
                     /locus_tag="PLV68_15355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02678.1"
                     /translation="GAYSRWGWHHPGPLLYYLLAPLTWVFGHSGALAGVVLINFACAA
                     AAVEATRRRLGHAAAAMVVAITVVLAWALGPDLLMNPWNPRVALIPLFACGVFAWLLA
                     GGWSKALPMVIALGSFVVQAHVGFAAVVVAIVAVAVALWLGVERLSRQVPSREVPSRQ
                     VPSRQVPSRQVPSRQIPSRQVPSRQVMVTSAVVMLVCWFGPLVETVVHWPGNLVEIAK
                     YFVRSDEPALGWGDAWRIFSTEVGLPGAWVRRSMLTLPLSADSPLPALIVLGAV"
     assembly_gap    1306..1434
                     /estimated_length=129
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1573..>1790)
                     /locus_tag="PLV68_15360"
     CDS             complement(1573..>1790)
                     /locus_tag="PLV68_15360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02679.1"
                     /translation="INLTAADQALPGFLTAFPSGSAMPKTSTLNFAAGEPVANGALLK
                     LPAGGALDVFALVQSAVIVDVNGYFTP"
     assembly_gap    1791..1981
                     /estimated_length=191
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1982..>2298)
                     /locus_tag="PLV68_15365"
     CDS             complement(<1982..>2298)
                     /locus_tag="PLV68_15365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02680.1"
                     /translation="LDTRPQHDGEPDVRIGGAAVVVPRPASVPTSGVAALVLNVTATE
                     ALVPGFLRAQPSGTTPPTSTVNVVPGVAAANTVIVPLGADGTVSIFASVPAHIVVDVT
                     GYI"
     assembly_gap    2299..2451
                     /estimated_length=153
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2452..2779)
                     /locus_tag="PLV68_15370"
     CDS             complement(<2452..2779)
                     /locus_tag="PLV68_15370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02681.1"
                     /translation="MGEVRLPQPPGPAASGVAASYVPLAPYRALDTRPASAVGPVASI
                     GALPPHGVIDVSLAGVGAVPATATAVAINLTSTDAVNPSYLQAVPYLHAALGSSSTLN
                     VAQAGAI"
BASE COUNT          434 a          876 c          756 g          256 t
ORIGIN      
        1 cacgccgagc aacacgccga cggagcaggc aacgaatacg ccaacggcta cgtcaacgcc
       61 cacgttcacg ccaacgaaca cgccgacgcc gacctcgccc cacgtgttgc gatcagccca
      121 ctcgaccacg tactgccgat ccgtagggag cacgccgtcg agttggtcga ccagcgcgat
      181 gatcgcctcg ctctcggccg gcgccggtgg atcggccgtc accgcgccga ccgtcaacgc
      241 aaccgacgcc accgccgcga caacacccgc gccgacagcc acccgtacct gccaccgtcg
      301 cgacaccagt gcggccgccg aaacgacgat gctcagccac atccacgcgc tgatcaccca
      361 ccaccagcgc acgatgtagt cgtgcggcag cccggtgaca cgggccgtcg ccacgaaccc
      421 ggcggccaga ccggccatgc ccagcaaggc caacgccgcg gcatnnnnnn nnnnnnnnnn
      481 nnnnnnnnca ccgcaccgag cacgatcagc gcgggcagtg gcgagtcggc gctgagcggc
      541 aaggtcagca tgctgcgccg gacccacgcc ccgggcaggc cgacttcggt ggaaaagatg
      601 cgccacgcat cgccccaacc gagcgcgggt tcgtcggagc gcacgaagta cttggcgatc
      661 tcgaccaggt tgccgggcca gtggaccacc gtctcgacca acggcccgaa ccaacacacc
      721 agcatcacga cggccgacgt gaccatgacc tggcgcgatg ggacctgacg cgatgggatc
      781 tgccgtgatg ggacctggcg cgatgggacc tgacgcgatg ggacctggcg cgatggcacc
      841 tcacgcgacg gcacctgacg cgacagccgc tcgacgccga gccacagcgc aaccgcgacc
      901 gcgacgatcg cgaccaccac cgccgcaaat ccgacgtgcg cctgcaccac gaagctgccc
      961 aacgcgatca ccatcggtag cgccttcgac cagccgccgg ccaacagcca cgcgaacacc
     1021 ccgcacgcga acagcgggat caatgcgacc ctggggttcc acgggttcat cagcaggtcc
     1081 ggccccaacg cccacgccaa caccaccgtg atcgcgacga ccatcgccgc cgcggcatga
     1141 ccgagacggc gccgtgtcgc ctcgaccgcg gccgcggcgc aggcgaagtt gatcagcacc
     1201 acgccagcga gggcgccgga atggccgaac acccacgtca gcggggccaa caggtagtac
     1261 agcagcggcc ccgggtggtg ccagccccag cgcgagtacg cgccgnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncggcac
     1441 cgaccggcaa caccgaccgc caccgcatga gcccgacgac acccaactcc cccgatggtg
     1501 acaccggagt cgacacgacg ccgacggccg gctcaccaac gttggtcggc ccagcgttcg
     1561 ttggatcggt gctcatggcg tgaagtagcc gttgacgtcg acgatcacgg cagactgcac
     1621 cagcgcgaac acgtccaacg cgccaccggc cggcagcttc agcagcgccc cgttggccac
     1681 cggttcgccc gcggcgaagt tgagcgtgga cgtcttgggc atcgcgctgc cactggggaa
     1741 cgcggtcaga aaccccggca acgcctggtc ggccgcggtg aggttgatcg nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 ngatgtaacc ggtcacgtcg acgacgatgt gcgcaggaac gctggcgaag atgctgaccg
     2041 tgccgtcggc gcccagcggc acgatcaccg tgttggccgc ggcgacgccg ggcacgacgt
     2101 tgaccgtcga cgtcggcggt gtggtccccg acggctgggc gcgcaagaac cccggcacga
     2161 gcgcctcggt cgcggtgacg ttgagcacca acgcggcgac cccgctggtc ggcaccgatg
     2221 ccggacgagg caccaccacg gccgccccgc cgatgcgcac gtcgggctcg ccgtcgtgct
     2281 gcggacgggt gtcgagggnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nggatcgcac
     2461 cggcctgagc gacgttgagc gtggacgacg aacccagcgc agcatgcaga tacggcaccg
     2521 cctgcaggta gctgggattg accgcatcgg tcgaggtgag gttgatcgcc accgccgtcg
     2581 cggtcgcggg caccgccccg acccccgcca acgacacgtc gatcacaccg tgcggaggga
     2641 gcgctccgat cgatgccacg gggccgacgg cgctcgccgg gcgcgtgtcc aacgcccggt
     2701 atggggccag cggcacatag ctcgccgcca cgcccgacgc ggccggtccg ggcggctgcg
     2761 gcagtcgcac ttcccccatg ccgtacacgt tgtcggggcc gtcgggaaac accagcgaa
//
LOCUS       DAORNF010000435         2818 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_26934_length_2818_cov_1.696345, whole genome shotgun sequence.
ACCESSION   DAORNF010000435 DAORNF010000000
VERSION     DAORNF010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2818
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26934_length_2818_cov_1.696345"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1020
                     /locus_tag="PLV68_15375"
     CDS             <1..1020
                     /locus_tag="PLV68_15375"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRE02682.1"
                     /translation="GPIVAIVLTELGPNATAAQYERARENMGLNLGLTERYFDWVGGA
                     VTGDLGESLFPPQRYVTDMVAERLPVTLELAVLALVLALLIAVPLALYTSTRPGSFID
                     GIATGGAFASLSTPSFLAGLLVVQFFVFQLGLVRWTLGIVAVAGGLALAWTARRADER
                     IRRLIWAFAIAAGGAALAILLPAFPRQGFARVSEAGVFENLRYLALPTLTLALMESAV
                     FLRLLRGDLIDTLKQDYILAARAKGMSMPHVMLRHALRPSCVSLVTVVSVTLGRLIGG
                     AVVIETIFNLPGIGRMLIEAIVNKDFPVVQAGVLVVAVVYVVMNIFVDLAYGVLDPRV
                     RRGAS"
     gene            1007..2089
                     /locus_tag="PLV68_15380"
     CDS             1007..2089
                     /locus_tag="PLV68_15380"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRE02683.1"
                     /translation="MAPLDVTVDVGAASGDAPPEVAGTPECAAAPSKKRLLWGVLAVV
                     VGVAACAAAGLIPTTAVVRIPLAVFGFGALVFALRLALVARGVRTPVGVVLATAWLTL
                     IVLATITADWLPLAEARDPSKTLRTPILARPGLGEHLLGTDRQGLDLLGGIVYGSRVS
                     LTVGVGAAVIAMIVGGAIGMVSGYFRGPLDRVIQFFMTSLLAFPPLILLMGLAAVLDP
                     TVPNVTMALMVVVIPVFARVARASTLVVAQQDYIQAAKVIGATHRRILVRDVIPAIVQ
                     SLISYALVMVSMLVVAEASLSYLGLGIPRPEPTLGNIISAGKDQFNKHPHLVFGPALA
                     LFLTVLSLNLLGEHLRKLWDRREAKV"
     assembly_gap    2195..2417
                     /estimated_length=223
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2418..>2818
                     /locus_tag="PLV68_15385"
     CDS             <2418..>2818
                     /locus_tag="PLV68_15385"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRE02684.1"
                     /translation="SHFDWLVDQGVPFRAAFWDDPTYEPWDDSGLMYSGGERAHPFCE
                     IARPVPRGHIPQASGTSRTGAGGGFKLIEALMARAGQLGVHLEVGASARVLDTDADGA
                     VVGVVARQLGEELCVRARHGVVLASGGFGFN"
BASE COUNT          336 a          837 c          894 g          528 t
ORIGIN      
        1 gggccgatcg tcgccatcgt tctcacggag cttggcccca acgccaccgc cgcgcagtac
       61 gaacgagccc gcgaaaacat gggcctgaat ctcgggttga ccgagcgcta cttcgactgg
      121 gtcggcggcg ccgtcacggg tgatctgggg gagtcgctct tcccgcccca gcgttacgtc
      181 accgacatgg tggcagagcg cctgcccgtg acgttggagt tggcggttct cgccttggtg
      241 ttggcgctct tgatcgctgt gccgctcgcg ttgtacacct cgacgcgtcc tgggtcgttc
      301 atcgacggga tcgccaccgg aggcgcgttt gcgagcttgt cgacgccgag ctttctggcc
      361 ggtctgctgg tggtgcagtt cttcgtgttc cagcttggtc tggtgcgatg gaccctgggc
      421 attgtcgcgg tcgcaggcgg gctggccttg gcgtggacag cacgacgtgc cgacgagcgg
      481 atccgccggc tgatctgggc gttcgcgatc gcggccggtg gggcggcgct cgccatcctg
      541 ctgcccgcct ttcctcgcca gggtttcgct cgggtgagcg aggccggggt gttcgagaac
      601 cttcgttatc tggcgctgcc gacgctgacg ctcgccttga tggagtcggc cgtgttcctg
      661 cgtctcttgc gcggtgacct gatcgacacg ctcaagcagg actacatcct cgccgcacgt
      721 gccaagggca tgtcgatgcc tcatgtgatg ctccgccacg cgctgcgacc gtcatgcgtg
      781 tcactggtga ccgtggtcag cgtcaccctc ggtcggttga tcggcggtgc cgtggtgatc
      841 gagacgatct tcaacctgcc gggcatcggc cggatgctga tcgaagcaat cgtcaacaag
      901 gacttcccgg tcgtgcaggc tggggttctc gtggtggccg tggtgtacgt ggtgatgaac
      961 attttcgtcg atctcgccta cggcgtgctc gatccaaggg tgcgccgtgg cgcctcttga
     1021 cgtcacggtc gacgtcggcg cagcgtcggg agacgctccc ccggaggtcg cgggaacccc
     1081 tgagtgtgcg gccgcgccat cgaagaagcg gttgctgtgg ggcgtcctgg ccgtggtcgt
     1141 cggcgtagcc gcctgcgccg ctgccggatt gatcccgacc accgccgtcg tccgcatccc
     1201 gctcgcggtc ttcgggtttg gcgcgctcgt cttcgcgctg cggctggcct tggtggcccg
     1261 tggcgtgcga accccggtcg gtgtcgtcct ggccacagcg tggttgacgt tgatcgtgct
     1321 cgccacgatc accgcggact ggctgccgct tgcggaggct cgcgacccgt cgaagacctt
     1381 gcgaacgccg atcctggccc gacccggcct gggcgaacac ctactcggaa ccgatcgcca
     1441 aggtctcgac ctgctcggcg gcatcgtgta cggctcgcgg gtgtcgctca ccgtcggcgt
     1501 cggcgctgct gtcatcgcga tgatcgtcgg tggcgcgatc gggatggtgt ccggctactt
     1561 ccgtgggccg ctcgatcgcg tgatccagtt cttcatgacg tcgctgttgg cgttcccgcc
     1621 attgatcctg ttgatgggtt tggccgccgt cttggacccg accgtcccca acgtgacgat
     1681 ggcgctgatg gtcgtggtga tccccgtgtt cgcccgggtc gcccgagcct cgacgttggt
     1741 ggtcgcccaa caggactaca tccaagcggc caaggtcatc ggggccacac accggcggat
     1801 cctcgtccgc gacgtcatcc ccgcgatcgt ccaatcgctg atctcgtatg cgttggtgat
     1861 ggtgtcgatg ctcgtcgtgg cggaagcatc gctgagctac ctcggtctcg gtatcccgcg
     1921 gcccgaacca acgctgggca acatcatcag tgccggcaaa gaccagttca acaagcaccc
     1981 gcacctcgtg ttcggccctg cgctggcatt gttcttgacc gtgctgtcgc tcaacctgtt
     2041 gggcgagcat cttcgcaagc tgtgggaccg gcgcgaagca aaggtctgac cgcggcagcg
     2101 gtcaccaacc gtcctcatca cgcacacgga gagcgatcga tcatgcgtat tctcgagacc
     2161 gatgtcgtcg tcgtcggctg cggcgcagcc ggggnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnngag ccacttcgac tggctcgtcg accagggggt cccgttccgg
     2461 gctgcgttct gggacgaccc gacctatgaa ccgtgggacg actccgggct gatgtactcc
     2521 gggggcgaac gggcccaccc gttttgcgag atcgcccggc ccgttccgcg ggggcacatt
     2581 ccccaggcgt ccgggacgtc gcgcaccggc gccggtggtg gtttcaagct gatcgaggcg
     2641 ctgatggcac gggcgggaca gctcggggtg cacctcgagg tgggcgcgtc ggcgcgagtt
     2701 ctcgacaccg atgctgacgg cgcggtggtc ggtgtcgtcg ctcgccaact gggcgaggag
     2761 ctgtgtgtgc gggcccgcca cggcgtcgtg ctggcgagtg gcggcttcgg tttcaacg
//
LOCUS       DAORNF010000436         2813 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_26995_length_2813_cov_1.674764, whole genome shotgun sequence.
ACCESSION   DAORNF010000436 DAORNF010000000
VERSION     DAORNF010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2813
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26995_length_2813_cov_1.674764"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1064
                     /locus_tag="PLV68_15390"
     CDS             <1..>1064
                     /locus_tag="PLV68_15390"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HRE02685.1"
                     /translation="ASDNDRFVALDLLKNANIAAGGVLPMCQDTGTAIVKAKKGQMVL
                     TRAGDGTLGGDEEAIARGVLNTYQTDNLRYSQMAPITMYDEVNTGNNLPAEIKISAID
                     GDAYKFLFMAKGGGSANKSYLFQETKALLNPKTLLPWIFEKLKTLGTAACPPYHLAVV
                     IGGTSAEFAVETAKLASTRYLDNLPTQGTKLGHAFRDVELEAAVLKLSQETGIGAQFG
                     GKYFCHDVRIIRLPRHGASCPVGMAVSCSADRQALGKITKDGIFIERLETDPARFLPD
                     VTHEHLDDGDGDAGVVRIDLNQPMDAIRAELSKYPVKTRVMLTGPMVVARDIAHAKIK
                     EMLDAGQPMPQYLKDYCVYYA"
     assembly_gap    1065..1102
                     /estimated_length=38
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1103..1469
                     /locus_tag="PLV68_15395"
     CDS             <1103..1469
                     /locus_tag="PLV68_15395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fumarate hydratase C-terminal domain-containing
                     protein"
                     /protein_id="HRE02686.1"
                     /translation="EGYASGSFGPTTAGRMDSYVDEFQSHGGSMVMLAKGNRSRQVTN
                     ACHTHGGFYLGSIGGPAARLALDCITQVDVLEFPELGMEAVWKIEVQDFPAFIVVDKG
                     NDFFDLVDRPYAGTPVALK"
     gene            complement(1503..>1766)
                     /locus_tag="PLV68_15400"
     CDS             complement(1503..>1766)
                     /locus_tag="PLV68_15400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02687.1"
                     /translation="VCAFVVGATTQQQRADRVFALPERDDTIAAIAPLLVAEGVPAPM
                     TECVAAGIVDNGEVRLVVHELLGVQSPALAALRAAQLRDCRGG"
     assembly_gap    1767..1786
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1787..2797)
                     /locus_tag="PLV68_15405"
     CDS             complement(<1787..2797)
                     /locus_tag="PLV68_15405"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRE02688.1"
                     /translation="MEADAFPQAILDRFDLVGFDPRGVGASSAVSCPVSFDITDADDV
                     SRCIDRSHDLLAHLGTPNVARDLELIRVALGDPQLSYLGFSYGTALGAVYATMFPASV
                     RALVLDGAIDPDAGIANEQAGAGYDFYVQQDFGTTLDVFFTLCDVSVLCPAGPDSESL
                     LDDVYDEVRNLPVAYFGDHEPLRRVDVEDVVYGAMYGIFTWPLLAHALSDAADGDAST
                     IAALASYLRYGYPADMEAEPNFEFANLAIRCADFSNRGADAFECAPLPDSADDLPVIT
                     AVDTPNPILVIGTDDDPATPGRYAGQLADALGDAVDINWAGAGHTAFLTSACVTDLTT
                     AYL"
BASE COUNT          498 a          942 c          933 g          382 t
ORIGIN      
        1 gccagcgaca acgaccgctt cgtggccctc gacctgttga agaacgccaa catcgcggcc
       61 gggggcgtgc tgcccatgtg tcaagacacg ggcaccgcga tcgtcaaggc caagaagggc
      121 cagatggtgt tgacccgcgc cggcgacggc acgctcggcg gcgacgagga agcgatcgcc
      181 cgcggcgtgt tgaacacgta ccagaccgac aacctgcgct acagccagat ggcgccgatc
      241 acgatgtacg acgaggtcaa cacgggcaac aacctgcccg ccgagatcaa gatcagcgcg
      301 atcgacggcg acgcgtacaa gttcctgttc atggccaagg gcggcggcag cgccaacaag
      361 agctatctgt tccaagagac caaggccctg ctcaacccca agaccctgct gccgtggatc
      421 ttcgagaagc tcaagacgct gggcaccgcg gcctgcccgc cgtatcacct cgccgtcgtc
      481 atcggcggca cgtcggccga gttcgcggtc gagacggcca agctggccag cacccggtac
      541 ctcgacaacc tgcccacgca gggcaccaag ctgggccacg cgttccgtga cgtcgaactg
      601 gaagccgccg tactcaagct cagccaggag accggcatcg gcgcccagtt cggcggcaag
      661 tacttctgcc acgacgtgcg catcatccgc ctaccccgcc acggcgcgag ctgcccggtg
      721 ggcatggcgg tcagctgtag cgccgatcgc caggcactcg gcaagatcac caaggacggc
      781 atcttcatcg agcggctgga gaccgatccg gcccggttcc tacccgacgt cacccacgag
      841 cacctcgacg acggcgacgg cgacgcgggg gtcgtgcgga tcgacctcaa ccagccgatg
      901 gacgcgatcc gcgccgagtt gagcaagtac ccggtcaaga cccgggtgat gctgaccggc
      961 ccgatggtgg tcgcccgcga catcgcccac gccaagatca aggagatgct cgacgccggc
     1021 cagccgatgc cgcagtacct caaggactac tgcgtctatt acgcnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nncgaggggt acgcgtccgg ctcgttcggc cccaccaccg
     1141 cgggccgcat ggacagctac gtcgacgagt tccagagcca cggcggcagc atggtcatgc
     1201 tcgccaaggg caaccgcagc cgccaggtca ccaacgcctg ccacacccac ggtgggttct
     1261 acctcggatc gatcggcggt cccgcggcgc gcctcgcgct cgactgcatc acccaggtcg
     1321 acgtgctcga gttccccgaa ctcggcatgg aggcggtctg gaagatcgag gtccaggact
     1381 ttcccgcgtt catcgtcgtc gacaagggca acgacttctt cgacctggtc gaccggccct
     1441 acgccggcac gcccgtcgcg ctgaagtagc cccgacgacg ccgcagcagg cgactgccgg
     1501 gctcagccac cccggcagtc gcgcagctga gcggcgcgca gcgcggccag cgccggtgac
     1561 tgcacaccca gcaactcgtg caccaccaga cgcacctcac cgttgtcgac gatgccagcc
     1621 gcgacacact cggtcatcgg cgcaggcacg ccttcggcga ccagcaacgg cgcgatcgcc
     1681 gcgatcgtgt cgtcgcgttc gggcagcgcg aacacgcggt cggcgcgctg ttgctgcgtg
     1741 gtggcgccga cgacgaatgc gcacacnnnn nnnnnnnnnn nnnnnncaga taggcggtgg
     1801 tcaggtcggt gacgcaggcg ctggtgagaa acgccgtatg gcccgcgcca gcccagttga
     1861 tgtcgaccgc gtcgccgagg gcatcggcca actgtccggc gtaacgaccg ggtgtggccg
     1921 ggtcgtcgtc ggtgccgatc accaggatcg ggttcggcgt gtcgaccgcg gtgatcaccg
     1981 gcaagtcgtc ggcgctgtcg ggcaacggcg cgcactcgaa cgcgtcggcg ccgcggttgg
     2041 agaagtcggc acagcggatg gcgaggttgg cgaactcgaa gttcggttcg gcctccatgt
     2101 cggccggata gccgtagcgc aggtagctgg cgagcgcggc gatggtcgac gcgtcgccgt
     2161 cggccgcgtc ggacaaggcg tgggccagca acggccacgt gaagatgccg tacatcgccc
     2221 cgtagacgac gtcttcgaca tcgacgcggc gcagcggctc gtggtcgccg aagtaggcca
     2281 ccggcaggtt gcgcacctcg tcgtacacgt cgtcgagcaa cgattcgctg tcgggcccag
     2341 ccgggcacag cacgctgacg tcgcacagcg tgaagaacac gtcgagcgtg gtgccgaagt
     2401 cctgctggac gtagaagtcg tatccggcgc cggcctgctc gttggcgatg ccggcgtcgg
     2461 ggtcgatcgc gccgtccagc accagggcgc gcacgctggc ggggaacatc gtggcgtaca
     2521 ccgcccccag cgcggtgccg tagctgaacc cgaggtagct gagttggggg tcgccgagcg
     2581 cgacgcggat cagctccaga tcacgcgcga cgttgggggt gccgagatgg gcgagcaggt
     2641 cgtgcgagcg gtcgatgcag cgggagacgt catcggcatc ggtgatgtcg aacgacacgg
     2701 ggcaactgac cgcgctggac gcaccgacgc cgcgcgggtc gaagccgacc aggtcgaagc
     2761 gatcgaggat cgcttgcgga aacgcgtcgg cttccaccgc cacgatcgcg atg
//
LOCUS       DAORNF010000437         2808 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_27058_length_2808_cov_1.769343, whole genome shotgun sequence.
ACCESSION   DAORNF010000437 DAORNF010000000
VERSION     DAORNF010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2808
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27058_length_2808_cov_1.769343"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>379
                     /locus_tag="PLV68_15410"
     CDS             <1..>379
                     /locus_tag="PLV68_15410"
                     /inference="COORDINATES: protein motif:HMM:NF024864.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HRE02689.1"
                     /translation="GATTTVEFEITLADISVSPNMIEVPAGALVKLKVTNTGTLAHDL
                     KVGDVGTRMLSRGESQDLTVGPFSEDSQAICTVPGHKDAGMTMMIHVVGGSGSGSGSG
                     HGDSGSGSGDEEDFAKIDSSKAPA"
     assembly_gap    380..460
                     /estimated_length=81
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <461..1256
                     /locus_tag="PLV68_15415"
     CDS             <461..1256
                     /locus_tag="PLV68_15415"
                     /inference="COORDINATES: protein motif:HMM:NF019352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HRE02690.1"
                     /translation="EELIEVAPGVTQMMWTFNGQVPGPTLRGKVGDTFRVTLTNTGQI
                     GHSIDFHASKVAWDDEMRTINTGESLVYEFTADHAGAFMYHCGTAPALHHIGNGMPGA
                     IIIDPPDLPEVDEEFVFVQSELYLGPEGKEGDLGKMLAEDWDAVVFNGYFNGYMHRPI
                     HVEVGARYRVWVVDDGPSENSAFHIVGTIFDTVFKEGAYTLQPDDTHGGSQAIDLQPS
                     QGGFVEFTFAEDGLYPFVTHKFANASKGALGFFAAGDADTSALGGH"
     assembly_gap    1551..1649
                     /estimated_length=99
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1650..2678
                     /locus_tag="PLV68_15420"
     CDS             <1650..2678
                     /locus_tag="PLV68_15420"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRE02691.1"
                     /translation="DRLGRKPMLLTGLAIFLAGAVISALMPTFGGLLFGRMLWGLGAS
                     APRSLAIAMVRDSQHGEQMARTMSLAMAVFLLVPIFAPTLGQVLISFGPWRIVFWVPA
                     VAALLLVVWIWRRLPESLPVERRRSVSPRALGVAFREVLRSRQTVLLGLAATFLYGAM
                     TSFLTNSEIVVEDVFGRGDIFALLFGLIGITLAAGSLSNANFVHRLGLVRMLRLNAIM
                     IVAAGVVIFAVTRIDGGHPPLWLFWLAVALFMPSAAMANPNCNTAAMLPVPHVAGMAA
                     AVIGAIATAGGAVLSSVVDGAFDGSVAPFGTFALVYVVAAAVCILAAIRQPAVFARTT
                     VITVADAH"
BASE COUNT          406 a          896 c          868 g          458 t
ORIGIN      
        1 gggcgcgacg accaccgtcg aattcgagat cacgttggcc gacatttcgg tcagtccgaa
       61 catgatcgag gtacccgcag gggcgctggt caagctgaag gtgacgaaca ccggcaccct
      121 cgcccacgac ctgaaggtgg gcgacgtcgg cactcgcatg ctcagccgcg gcgagtcgca
      181 agacctgacc gtcggtccgt tcagcgagga cagccaagcg atctgcacgg tgcccggcca
      241 caaggacgcg gggatgacga tgatgatcca cgtcgtcggt ggttcgggct cgggttcagg
      301 ctcgggccac ggcgactcgg gctcgggctc gggtgacgag gaggacttcg ccaagatcga
      361 ctcgtcgaag gctcccgccn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cgaagaactg atcgaggtcg
      481 cccccggcgt gacccagatg atgtggacgt tcaacggcca ggtccccgga ccgacgctgc
      541 gcggcaaggt cggcgacacg ttccgggtca cgctgaccaa cacgggccag atcggacaca
      601 gcatcgactt ccacgccagc aaggtggcct gggacgacga gatgcgcacg atcaacaccg
      661 gcgagtcgtt ggtctacgag ttcaccgccg accacgccgg ggcgttcatg taccactgcg
      721 gtacggcacc ggcactgcac cacatcggca acggcatgcc cggggcgatc atcatcgacc
      781 cgcccgatct gcccgaggtc gacgaggaat tcgtgttcgt gcagagcgag ctgtacctcg
      841 gccccgaagg caaggaaggc gacctgggca agatgctggc cgaggactgg gacgcggtcg
      901 tgttcaacgg ctacttcaac ggctacatgc accgcccgat ccacgtcgag gtcggcgccc
      961 gctaccgggt gtgggtcgtg gacgacggcc cgagcgagaa ctcggcgttc cacatcgtcg
     1021 gcacgatctt cgacaccgtg ttcaaggagg gcgcctacac gctccagccc gacgacaccc
     1081 acggcggctc gcaggcgatc gatctgcaac cgtcgcaagg tggcttcgtc gagttcacct
     1141 tcgccgagga cggtctctac cccttcgtca cccacaagtt cgccaacgcc agcaagggtg
     1201 cgctcggttt cttcgccgca ggcgacgccg acacctcggc cctcggcggc cactgatcac
     1261 actgatcacc agacgccggc cctggttcat ggtttcaccc tcaccatgag ccggggcggt
     1321 gaccgctgac cagccccgat gcccgaactg agacgcggcg tcggggcagg tcagcggtac
     1381 tgtgggccac cgtgtctgct gatgtgtcaa ccgttccgat ccaggcggtg acacggcaca
     1441 tcaagccatt gacacccagg ggcttcatcg ccgccatcgc gggttgcatg gcggtgagcg
     1501 cgttatcgat cgacctcgcg ctaccggcct ttccccagat ccgccagacc nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnng accgcctcgg tcgcaagccg atgttgctga
     1681 ccgggctggc gatcttcctg gcgggcgccg tgatctcggc gctgatgccg acgtttgggg
     1741 gcctgctctt cggccggatg ctgtgggggt tgggtgcctc ggccccacgt tcgctggcaa
     1801 tcgcgatggt ccgcgacagc cagcacggcg agcagatggc acgcacgatg tcgctggcga
     1861 tggcggtgtt cctgttggta ccgatcttcg cccccacgct gggccaggtc ctcatctcgt
     1921 tcggtccgtg gcgcatcgtg ttctgggtgc ccgcggtggc ggcattgctg ctggtggtct
     1981 ggatttggcg gcggctgccc gagagtctcc ctgtggagcg ccgtcggtcg gtctcaccca
     2041 gagccctcgg cgtcgcgttc cgcgaggtgt tgcgatcgcg ccagaccgtg ctgctcggcc
     2101 tggctgccac cttcctgtac ggggcgatga cgtcgtttct caccaacagc gagattgtcg
     2161 tcgaagatgt cttcggccgc ggcgacatct tcgccctgct gttcgggctg atcggcatca
     2221 cgctcgcggc cgggtcgttg tccaacgcca acttcgtcca tcgcctcggc ctggtccgca
     2281 tgctgcgact caatgcgatc atgatcgtcg ccgccggcgt ggtgatcttc gcggtcacgc
     2341 gcatcgacgg tggccaccca ccgctgtggc tgttctggct cgccgtggcc ctgttcatgc
     2401 cgagcgcagc gatggccaac cccaactgca acacggcggc gatgttgccc gtgccgcacg
     2461 tcgcgggcat ggccgcggcg gtcatcggtg ccatcgccac cgctggtggc gcggtgctca
     2521 gctcggtcgt cgacggagcc ttcgacgggt cggtcgcgcc gttcggcacg ttcgcgctgg
     2581 tctacgtcgt cgcagccgcg gtctgcatcc tggccgcgat ccgccaaccg gctgtgttcg
     2641 cgcgcacgac ggtcatcacc gtcgccgacg cccactgacg caagccgaaa tctgctgccg
     2701 cgtcaggcgt cggcaggcgg ggccagctcg acttcgaccg acggctcggc gccggatggg
     2761 gtcgccagca actccagcgc ttcgaccaca ccggcgcggt ggacgtcg
//
LOCUS       DAORNF010000438         2806 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_27082_length_2806_cov_2.081788, whole genome shotgun sequence.
ACCESSION   DAORNF010000438 DAORNF010000000
VERSION     DAORNF010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2806
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27082_length_2806_cov_2.081788"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="PLV68_15425"
     CDS             complement(<1..189)
                     /locus_tag="PLV68_15425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02692.1"
                     /translation="MSDGDVGRSRPGGTQHIPRPAAWSLGGAAPWSVPSPRSADAVAA
                     LVGATEQTRQSGLPTFPSA"
     gene            complement(199..366)
                     /locus_tag="PLV68_15430"
     CDS             complement(199..366)
                     /locus_tag="PLV68_15430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02693.1"
                     /translation="MTFERPPADLSKLVTAWHEWERGEESPGKVLANMKTAGLAEVLD
                     ELVRTGWTPSA"
     gene            complement(550..690)
                     /locus_tag="PLV68_15435"
     CDS             complement(550..690)
                     /locus_tag="PLV68_15435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02694.1"
                     /translation="MTPNDTERRRDVGESIVESYRLHPQDEADDEWAMTNALALTEAE
                     PW"
     gene            complement(687..2093)
                     /locus_tag="PLV68_15440"
     CDS             complement(687..2093)
                     /locus_tag="PLV68_15440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HRE02695.1"
                     /translation="MTPEDFRRNGHALVDWIADYIETVGQRPVTDPTLAPGDVRRQLP
                     PHPPSSPEPFDAVMADMDRVILPTITHWQHPQFFAYFPANTSYPSILAELATAGLGIQ
                     GMSWVTSPACTEVEALMLDWMQELLGLPAVFRNDGGVGGGVIHGSASEATLAAILAAR
                     CRITDGAVNAFADTTKLVAYITSQAHSGLEKGLRIAGIGTEQTRIVDHDEMFAMRASH
                     LDALIAQDRAAGLTPFFVCATRGTTSSTAFDPVPEIGAIARREGLWLHVDGAMSGIAA
                     LVPEYRWVNDGMELADSYCTNPHKWMGINFDCDLFYTADRAALLGALSILPEYLRSAA
                     LDADAAVDYRDWQIPLGRRFRALKLWFSLRCDGVAPIQAMIREHVRLTQLLAEWVAAD
                     DRFQIDAPHPLNLLCLSLRSGSAETDALITRANATGQALFTRTVLDGNVSLRVSIGAR
                     TTEHPHVQSAWHLLQSLA"
     gene            2147..>2806
                     /locus_tag="PLV68_15445"
     CDS             2147..>2806
                     /locus_tag="PLV68_15445"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRE02696.1"
                     /translation="MSDAETVPADIDPDRFAVSDASPRGFAQAYVRVNAGGVPLLCVH
                     GWPESKRIFWKVIEPLAAAGFEVIVPDLRGFGDSEVATDAADGGALHDVYAHALDLYA
                     LVHDVLGHERVVLMGGDLGGPVVQYLALRYPQWVDRMVLFNSPLPYLPDRMAHIANTR
                     ASREASDYFLRQGTDADALAAEFTTPEQRRRYIATFYTSRFWAHPGAFATQAEVDFHT
                     EP"
BASE COUNT          435 a          909 c         1003 g          459 t
ORIGIN      
        1 ggcactcgga aaggtcggca acccggactg gcgtgtctgc tcggtggcgc cgaccagcgc
       61 ggccacagcg tcggccgagc gcggcgacgg cacgctccac ggcgcggcac cacccaggct
      121 ccacgccgca ggccgcggaa tgtgctgggt gccgcccggc ctggatcgac cgacgtcgcc
      181 gtcgctcacg tcagtcgctc aggcggacgg cgtccagccg gtgcgcacca gttcgtcgag
      241 cacctcggca aggcctgcgg ttttcatgtt ggcgagcacc ttgcccggcg actcttcgcc
      301 ccgttcccac tcgtgccacg cggtgacgag cttggacaag tcggctggcg gtcgttcaaa
      361 cgtcataccc ccaggctacg cgccaacgcc gatggcgcac cctggcgtcg gtgagcggcg
      421 gacaaggccg cgttcgtcgc gcgggagatt cgtaccgccg aggccgattt gcagtgcttc
      481 atcgatcacc ttgccgtcgt gaaggacacg gtcgagcgcc gggttggcgc cgcgggtcag
      541 cagtcggcgt caccatggtt cggcttcggt cagcgccaac gcgttggtca tcgcccattc
      601 gtcgtctgcc tcgtcttgcg gatggagccg atacgactcg acgatcgact ccccaacatc
      661 ccgccgccgt tcggtgtcgt tcggtgtcat gcgagggatt ggaggaggtg ccaggcggat
      721 tggacgtggg ggtgttcggt ggtgcgggcg ccgatggaga cgcgcaggct gacgttgccg
      781 tcgagcaccg tgcgggtgaa cagcgcttgg ccggtggcgt tggcgcgggt gatcagcgcg
      841 tctgtctcgg cgctgcctga gcgcaacgag aggcagagca ggttcagcgg gtgcggcgcg
      901 tcgatctgga accggtcgtc ggccgcgacc cactcggcca gcagttgggt gagccgaacg
      961 tgctcgcgga tcatcgcttg gatcggggcg acaccgtcgc accgcagcga gaaccacagc
     1021 ttcagcgccc ggaagcggcg gccgagcgga atctgccagt cgcggtagtc gaccgccgca
     1081 tcggcatcga gggcagccga acggagatac tcgggcagga tgctcagcgc gcccaacagc
     1141 gcagcgcggt cggcggtgta gaacaggtcg cagtcgaagt tgatgcccat ccacttgtgc
     1201 gggttggtgc agtagctgtc ggccaactcc atcccgtcgt tgacccagcg gtactcggga
     1261 acgagcgcgg cgatgccgct catcgcgccg tcgacgtgca gccacagtcc ttcccgacgg
     1321 gcgatggcac cgatctcggg taccggatcg aacgcggtcg aactggtggt gccacgggtg
     1381 gcgcagacga agaacggcgt caggccagcg gcccggtcct gagcgatcag cgcgtcgaga
     1441 tgcgaagcgc gcatcgcgaa catctcatcg tgatcgacga tgcgggtctg ctcggtaccg
     1501 atcccggcga tgcgcagccc cttctccagt ccgctgtggg cctgtgacgt gatgtacgcg
     1561 accagcttgg tggtgtcggc aaaggcgttg accgcgccgt cggtgatgcg gcaacgggcg
     1621 gccaggatcg cggccagcgt cgcttccgac gccgagccgt ggatcacgcc gccgccgaca
     1681 ccgccgtcgt tgcggaacac cgcgggcaga ccgagcagtt cctgcatcca gtcgagcatc
     1741 agcgcttcga cctccgtgca ggcagggctg gtcacccaac tcatgccttg gataccgagc
     1801 ccggccgtgg ccagttcggc caggatcgac ggatagctgg tgttggccgg gaagtaggcg
     1861 aagaactgcg ggtgctgcca gtgggtgatc gtcggcagga tgacgcggtc catgtcggcc
     1921 atcaccgcgt cgaacggctc gggtgacgac ggcgggtgcg gcggaagttg acggcgcaca
     1981 tcgccgggag ccagggtggg atcggtgacc ggtcgctgac cgacggtctc gatgtagtcg
     2041 gcgatccagt cgaccaacgc atggccgttg cggcgaaagt cctcgggggt caccggttca
     2101 ggctatgcac gccggtgtcg cctcaagagt gtcgtaggtt gcagagatga gcgacgccga
     2161 gaccgttccc gccgacatcg accctgaccg gttcgcggtc agcgacgcat cgccgcgggg
     2221 attcgctcag gcgtacgtgc gggtcaacgc cggcggggtc ccactgctgt gcgtgcacgg
     2281 ttggccggag tcgaagcgga tcttctggaa ggtgatcgag ccgctcgccg cggcggggtt
     2341 cgaggtgatc gtgcccgacc tgcgcggctt cggcgacagc gaggtcgcca ccgacgcggc
     2401 cgatggggga gcgctgcacg acgtatacgc ccacgccctc gatctgtacg cgctggtgca
     2461 cgacgtgctc ggtcacgagc gagtggtgtt gatgggcggc gacctcggtg ggccggtcgt
     2521 gcagtacctg gccctgcgct acccgcagtg ggtcgaccgg atggtgttgt tcaactcgcc
     2581 gctgccgtac ctgcccgacc ggatggcgca catcgccaac acgcgcgcgt cgcgggaggc
     2641 atccgactac ttccttcgcc aaggcaccga cgccgatgcg ctggcagcgg agttcacgac
     2701 gcccgagcag cgacggcgct acatcgccac cttctacacc agtcgcttct gggcgcatcc
     2761 tggtgcgttc gcgacgcagg ccgaggtgga ctttcacacc gagccc
//
LOCUS       DAORNF010000439         2802 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_27139_length_2802_cov_1.137241, whole genome shotgun sequence.
ACCESSION   DAORNF010000439 DAORNF010000000
VERSION     DAORNF010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2802
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27139_length_2802_cov_1.137241"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="PLV68_15450"
     CDS             <1..188
                     /locus_tag="PLV68_15450"
                     /inference="COORDINATES: protein motif:HMM:NF014698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HRE02697.1"
                     /translation="VLLEPGDLLIVDNSMVVHGRSPFTPRFDGTDRWLQRAFVVDDLA
                     PSAAERDGRVITTVFGA"
     assembly_gap    388..416
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <417..>629
                     /locus_tag="PLV68_15455"
     CDS             <417..>629
                     /locus_tag="PLV68_15455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HRE02698.1"
                     /translation="FGREVLALLVWNGTGANVAALASMLGRHQAVICSNWSHINVDET
                     AAPERILGTKLIDLDCPDGKLTPAQV"
     assembly_gap    630..814
                     /estimated_length=185
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <815..1323
                     /locus_tag="PLV68_15460"
     CDS             <815..1323
                     /locus_tag="PLV68_15460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="threonine aldolase"
                     /protein_id="HRE02699.1"
                     /translation="LGGDRATLRAMTVEAGVDVVTFGGTKNGMMYGEAVVVLDDRFAT
                     AAPYLRKQVTQLPSKMRYVAAQFNAMFRDDLWLTTATHSNAMADRLYVATADIAGVAY
                     DTAPVVNSVFPRLSRAAAEELRSWCFFWDWDTSVDQVRWMTAWDTTVADIDAFALGVR
                     HVMGARGA"
     gene            1548..1802
                     /locus_tag="PLV68_15465"
     CDS             1548..1802
                     /locus_tag="PLV68_15465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HRE02700.1"
                     /translation="MTDAACKGLTNLFFPPPAERPQARDRREAMARSVCEACRVHDTC
                     QGYARDNHEYGFWGGESEDERHSAGFRLIAPIGVRARSAS"
     gene            1832..2077
                     /locus_tag="PLV68_15470"
     CDS             1832..2077
                     /locus_tag="PLV68_15470"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HRE02701.1"
                     /translation="MFDVCIVGSTNLDLVATAARLPGPGETVLGRDYAEHPGGKGLNQ
                     AVAAVAARVVLVTAGLPLVLKDAAPPKQTLSVKEQAS"
     gene            2074..>2288
                     /locus_tag="PLV68_15475"
     CDS             2074..>2288
                     /locus_tag="PLV68_15475"
                     /inference="COORDINATES: protein motif:HMM:NF014542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRE02702.1"
                     /translation="MKIPATVVTGFLGAGKTSLIRHLIANAGGRRLAVVINEFGDLGV
                     DRELLLGCGIAGCDEGDLIELPNGCIC"
     assembly_gap    2289..2502
                     /estimated_length=214
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2503..>2802
                     /locus_tag="PLV68_15480"
     CDS             <2503..>2802
                     /locus_tag="PLV68_15480"
                     /inference="COORDINATES: protein motif:HMM:NF014542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRE02703.1"
                     /translation="EAVQAERAADPAVLHEQPLQELFEDQLACADLIVLNKIDGLDDR
                     ALGAVSAELASRLRPEVKVVMTVRGTLDPLVALGIQAATEESIDGRWSHHDDEEDH"
BASE COUNT          386 a          781 c          795 g          412 t
ORIGIN      
        1 gcgtgttgct cgaacccggc gacttgttga tcgtcgacaa ctcgatggtg gtccacgggc
       61 gcagcccctt cacccctcgg ttcgacggta ccgaccgctg gttgcagcgc gccttcgtcg
      121 tcgacgacct ggcccccagt gcggccgagc gcgacggccg ggtcatcacg accgtcttcg
      181 gtgcgtgagg catcatcggc ggtcgagagg cttcggcgcc tgatcgcgtg gccggacata
      241 ctggccgcat gatcgatctc ccagccccat cgcgcagctt cgccagcgac aacgcggccg
      301 gcgttcaccc ggcggtcatg gaagcgctgg cggcggccaa ccacggccac gctctggctt
      361 acggcgacga tcgctggacc cgtgagtnnn nnnnnnnnnn nnnnnnnnnn nnnnnngttc
      421 ggccgcgagg tcctcgcgct gctggtgtgg aacggcacgg gggccaacgt cgctgcgttg
      481 gccagcatgc ttggacgcca tcaggcggtg atctgctcca actggtctca catcaacgtc
      541 gacgagaccg ccgcgcccga gcgcatcctc ggcaccaagc tgatcgacct cgactgcccc
      601 gacggcaagc tgaccccggc ccaggtcgan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnccttgg gcggcgatcg ggccacgttg
      841 cgggcgatga cggtcgaggc cggcgtcgac gtcgtcacct tcggtggcac caagaacggg
      901 atgatgtacg gcgaagccgt ggtcgtgctc gacgaccggt tcgccaccgc cgcgccctac
      961 ctgcgcaagc aggtcaccca actaccgtcc aagatgcgct acgtcgcggc ccagttcaac
     1021 gcgatgttcc gcgacgacct gtggctgaca acggcgacgc actccaacgc gatggccgac
     1081 cggctgtacg tcgcgaccgc cgacattgcc ggtgtcgcct acgacaccgc cccggtggtc
     1141 aacagcgtgt tcccccgcct gtcccgggca gcggcagagg aactgcgctc gtggtgcttc
     1201 ttctgggact gggacacgtc ggtcgaccag gtgcggtgga tgaccgcatg ggacaccacc
     1261 gtcgccgaca tcgacgcgtt cgcgctcggt gtgcgccatg tgatgggcgc gcgcggcgcc
     1321 tgaacgacct gggcgggggc cagatcgggg tgcgatcgca ggccgatcga aaaatccttg
     1381 cgcgatacaa ttgactctgc gatcacttct tctctacaat cgtgatcgtt ggttcggcag
     1441 gggtggtcga gtccagcgct tcatacgaat cgatgctctg gggtctaggg ggttccatgg
     1501 ctgctgttga gatcttgatc caccgcgagg ccaccgatga tcggtggatg accgacgcgg
     1561 cgtgcaaagg actcaccaac ttgttctttc cgccgcctgc tgaacggcca caagctcgcg
     1621 atcggcgcga ggcgatggcc cgctcggtgt gcgaggcctg ccgggtgcac gacacctgcc
     1681 agggctacgc ccgcgacaac cacgagtacg gcttctgggg cggcgagtcc gaagacgagc
     1741 gccacagcgc tggcttccgc ctgatcgcgc cgatcggtgt gcgtgcccgc agcgcgagtt
     1801 gatccacgct ggcacggcca tactgggcgc cgtgtttgac gtctgcatcg tcgggagcac
     1861 caatctcgat ctggtcgcta cggcggcccg actgcccggc ccgggtgaga cggtgctcgg
     1921 gcgtgactat gccgagcacc cgggtggcaa gggtctcaat caggccgtcg ccgccgtcgc
     1981 agcgcgcgtc gtccttgtca ccgcgggcct gccgctggtc ctgaaagacg cggcgcctcc
     2041 gaaacagacc ctcagcgtga aagaacaggc ctcgtgaaaa ttcccgcaac cgtcgttact
     2101 ggcttccttg gcgccggcaa gacctcgctc attcgccacc tcattgccaa tgccggaggc
     2161 cggcggctgg cggtcgtcat caacgaattc ggtgatctcg gtgtcgaccg cgagctgttg
     2221 ctcggatgcg gaatcgccgg ttgcgacgag ggcgacctga tcgaactgcc caacggctgt
     2281 atctgctgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngaggcggt gcaggcggaa
     2521 agggcggcgg atccggccgt tttacacgag cagccattgc aggagctgtt cgaagatcag
     2581 ctcgcgtgcg ccgacctgat cgttttgaac aagatcgacg gactggacga tcgcgcgctg
     2641 ggcgcggtga gtgccgaact ggcctcgcgt ttgcggcccg aggttaaggt ggtgatgacg
     2701 gttcgcggca cgctggatcc cctcgtcgcg ctcggcattc aagcggcgac ggaagagtcg
     2761 atcgacggcc gctggtcgca tcacgacgac gaggaagacc ac
//
LOCUS       DAORNF010000440         2798 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_27187_length_2798_cov_1.912140, whole genome shotgun sequence.
ACCESSION   DAORNF010000440 DAORNF010000000
VERSION     DAORNF010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2798
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27187_length_2798_cov_1.912140"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /locus_tag="PLV68_15485"
     CDS             complement(<1..427)
                     /locus_tag="PLV68_15485"
                     /inference="COORDINATES: protein motif:HMM:NF025052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HRE02704.1"
                     /translation="MGDPALHLIAGPNGAGKSTLHDRIIGPSTGLEFVNADVIASQRW
                     PGDELSHAYEASALAAARRAELLAAQSSFVTETVFSHRSKLDLIDDALAAGYLVTLHV
                     VIVPVELSVARVANRVTVGGHDVPVDKVRGRFARLWPLVA"
     gene            complement(427..807)
                     /locus_tag="PLV68_15490"
     CDS             complement(427..807)
                     /locus_tag="PLV68_15490"
                     /inference="COORDINATES: protein motif:HMM:NF023331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02705.1"
                     /translation="MASTSPARIDDDLYASAKLVGDLESRSASQQVAHWARIGRELER
                     SGRVPLRVVREVLASSRSYDTLTVEEQAGVRAEWSTLMRERRETLDLAREFAAEGRTW
                     VELDDDGSVVERAPVARRGRRSRT"
     gene            complement(855..>1169)
                     /locus_tag="PLV68_15495"
     CDS             complement(855..>1169)
                     /locus_tag="PLV68_15495"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02706.1"
                     /translation="GEQLGKPAGHDMVEGVYTLPVLRTLADGGVAADELGDLLGRPLD
                     PVTRDKALSIVRGGDGVAQAIDAAGAYVVAARDACALFPPSAATEAMLAAPQHLLDSI
                     RD"
     assembly_gap    1170..1179
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1180..1874)
                     /locus_tag="PLV68_15500"
     CDS             complement(<1180..1874)
                     /locus_tag="PLV68_15500"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HRE02707.1"
                     /translation="MSSTPFFALPGMPEVRQRIEDALVAAVDTRDDYLNELAKHLIVA
                     GGKRLRPVLTVVGAQVTGMPVTDAAIQGGIACELVQTGSLYHDDVIDESSTRRGVETV
                     NAKWGNLQAILAGDFLLARASEIAASLGAEVAGLLGHTIARLCEGEIQQLRHTYDVAR
                     PEASYLASIAGKTASLFSAAARIGGIVADLDRTVIDALTSYGLAYGMIFQIVDDVLDV
                     IATDPRCARLRQPL"
     gene            2060..>2798
                     /locus_tag="PLV68_15505"
     CDS             2060..>2798
                     /locus_tag="PLV68_15505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HRE02708.1"
                     /translation="MFERFTDRARRVVVLAQEEARLLNHSYIGTEHILLGLIHEGEGV
                     AAKAMESLGISLEAVRSQVEEIIGQGGSSPSGHIPFTPRAKKVLELSLREALQLGHNY
                     IGTEHILLGLIREGEGVAAQVLVKLGADLSKVRQQVIQLLSGYQGGQGKPGEPQAPAA
                     TGAPGKDDSGDKGNSQILDQFGRNLTQLAREKKLDPVIGRSRELERVMQILSRRTKNN
                     PVLIGEPGVGKTAIVEGLAQRIVSNDIP"
BASE COUNT          508 a          955 c          916 g          409 t
ORIGIN      
        1 gagcgacgag tggccacaga cgggcgaagc gcccgcgcac cttgtccact ggcacgtcgt
       61 gaccaccgac ggtgacccgg ttggcgacgc gggcgacgct caactcgaca ggcacgatca
      121 ccacgtgcag ggtgacgaga tatcccgcgg ccaacgcgtc atcgatcagg tcgagcttgg
      181 atcgatgtga gaagactgtc tcggtgacaa acgagctctg ggctgccaac agttcggcgc
      241 gtctcgcggc tgccaacgcg gacgcctcgt aggcatggga caactcgtcg ccgggccacc
      301 gctgggaggc gatcacgtcg gcgttcacga actccaaccc tgtcgacggg ccaatgattc
      361 gatcgtgcaa cgtcgacttg cctgcgccgt ttggaccggc gatcagatgg agcgctgggt
      421 cacccatcag gtccggctgc gacgcccgcg tcgggcgaca ggagcacgtt cgacgacgct
      481 gccgtcatcg tcgagttcaa cccacgttcg accctcggcc gcgaactcgc gggcaagatc
      541 gagtgtctcg cgacgctcgc gcatcagtgt tgaccactcg gcccgcacgc cagcctgctc
      601 ttcgacggtc aacgtgtcat acgaacgact ggacgcgagc acctcacgaa ccaccctcag
      661 cggaacgcgg ccagaccgtt ccagctcgcg tccgatccgc gcccaatgcg ctacttgttg
      721 cgacgcgctg cggctttcga gatcaccgac caatttggcc gacgcgtaga gatcgtcatc
      781 gatgcgggcc ggagaggtcg aagccatgca ccaaccgtag cactctgcaa caacgcccgg
      841 acagagcgcg aaggtcagtc gcggatggag tcgaggaggt gttggggggc ggccagcatc
      901 gcctcggtcg ccgcgctggg gggaaacagt gcgcacgcat cacgggcagc gaccacgtag
      961 gccccagcag cgtcgatggc ctgggcgaca ccgtcgccgc cgcgcacgat cgacagcgcc
     1021 ttgtcgcgcg tgaccgggtc gagcgggcgg ccgagcagat cgcccaactc gtcggccgcg
     1081 actcccccgt ctgccagggt gcgcaacacc ggcaacgtgt acacgccctc gaccatgtcg
     1141 tggccggccg gcttgccgag ctgctcgccn nnnnnnnnna gcggttggcg cagcctggcg
     1201 caccgcggat cggtggcgat cacgtcgagc acgtcgtcga cgatctggaa gatcatcccg
     1261 taggccaacc cgtacgaggt cagcgcatcg atcaccgtac ggtcgagatc ggcgacgatg
     1321 ccgccgatgc gggccgcggc gctgaacagc gaggcggtct tgcccgcgat cgacgccaag
     1381 tacgacgcct cgggccgggc gacgtcgtag gtgtgacgga gctgttgaat ctcgccctcg
     1441 cacaaccggg cgatggtgtg gcccaacaac ccggccacct cggcgccgag cgacgcggcg
     1501 atctcggacg cacgggcgag cagaaagtcg cccgcgagga tcgcttgcag gttgccccac
     1561 ttggcattga ccgtctcgac accgcggcgg gtggaggact cgtcgatcac gtcgtcgtgg
     1621 tagagcgacc cggtctgtac caattcgcac gcgatgccac cctggatcgc ggcgtcggtg
     1681 accggcatgc cggtgacctg cgcgcccacc acggtgagta ccggccgcag gcgcttgccc
     1741 ccggcgacga tcaagtgctt ggccaactcg ttgagatagt cgtccctggt gtcgacagca
     1801 gccaccagcg catcctcgat gcgctggcgc acctcgggca tgccggggag agcgaagaac
     1861 ggcgtgctcg acacgacggc caggctaggc ggtccgctcg ttgagcgcgc aaacaggtct
     1921 cccggaccca tcatcacgac ctgtcgtcgc tggccgtgct cccgcaccgg ccggcgcacg
     1981 ccgcggtggg tcgcgacggg ctccggacat gcgccgttac acttcccaac acgtatccac
     2041 cgcttcggaa agggcgggca tgttcgaacg cttcacagac agggcccggc gggtcgtggt
     2101 gttggcccag gaagaggcgc ggctcctcaa ccacagctac atcggcaccg aacacatttt
     2161 gctcgggctg atccacgaag gcgaaggcgt cgcggccaag gcgatggaat cgctgggcat
     2221 ctcgctcgaa gccgtgcgca gccaggtcga agaaatcatc ggccagggcg gctcgtcacc
     2281 gtcgggtcac atccccttca caccgcgcgc caagaaggtg ctggaactgt cgctgcgcga
     2341 ggcgctgcaa ctgggccaca actacatcgg caccgagcac atcctgctcg ggttgatccg
     2401 tgaaggcgaa ggcgtcgcgg ctcaggtgtt ggtcaagctc ggcgccgatc tcagcaaggt
     2461 gcgccagcag gtcatccagc tcttgtccgg ctaccagggt ggccagggca agcccggcga
     2521 accgcaggcg cctgccgcca ccggcgcgcc cggcaaggac gattcgggcg acaagggcaa
     2581 cagccagatc ctcgatcagt tcggtcgcaa cctcacccaa ctcgcccgcg agaagaagct
     2641 cgacccggtc atcggccgca gccgcgagct cgagcgggtg atgcagatcc tcagccgccg
     2701 caccaagaac aacccggtgc tcatcggcga gccaggcgtc ggcaagaccg ccatcgtcga
     2761 aggcctggcg cagcggatcg tctccaatga catccccg
//
LOCUS       DAORNF010000441         2795 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_27225_length_2795_cov_1.875182, whole genome shotgun sequence.
ACCESSION   DAORNF010000441 DAORNF010000000
VERSION     DAORNF010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2795
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27225_length_2795_cov_1.875182"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..988
                     /locus_tag="PLV68_15510"
     CDS             <1..988
                     /locus_tag="PLV68_15510"
                     /inference="COORDINATES: protein motif:HMM:NF014731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HRE02709.1"
                     /translation="LARVKWRHAVRQMTVEGKEKGVEAAELTKRLSLDSLTGQLNRAA
                     FQERLQALATRDGGVGLVVLFQNSSNLAAAYGIAVTTTMTITTLLFYVLAVYRWHWSK
                     AKALALVIPFLIVDLTFLGANIPKIPKGGWFPLTIAFLLVIQMSTWRRGRELVAARIH
                     RGERDIVDVLDEFSDVVRVPGSAVFMFKDLGKAPPALVNNLRHNKVLHRTTLIVSVTT
                     VEVPRNDPSKRHEVTKVEPGVYQVLLKFGFMEEPDVVAALSELKVRGLDFDPSTATFF
                     VGHESIIAGKAPGMWGPREHLFVILNRGADSASRFFRLPNDRIFEVGSQVEI"
     gene            1086..>2550
                     /gene="acs"
                     /locus_tag="PLV68_15515"
     CDS             1086..>2550
                     /gene="acs"
                     /locus_tag="PLV68_15515"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HRE02710.1"
                     /translation="MASEQAHDAIDALGWEQRTFPPSEEFKRDALVRGTAMYDALADY
                     QGFWARQAADLLDWSKDWHTICDWDLPDAKWFVGGELNVSHNCLDRHVAAGRGDKVAI
                     HFEGEPGDTRTITYADLLAEVQRFANVLRSLGIAKGDRVNIYLPMIPEAAVAMLACAR
                     IGAPHSVVFGGFSANALTDRINDAEAKLLITADGGYRRGEVFPLKPTADEALETTPSI
                     EHVVVVRRGGNKVTMQPGRDLWYHELMAAADPLCPAEPMDSEQLLFLLYTSGTTGSPK
                     GIMHTTGGYLTQVAFTHKYVFDLHVDTDVYWCTADVGWVTGHSYIVYGPLANGATQVM
                     YEGVPNFPGNDRFWSIIEKYKVTLFYTAPTAIRTFMKWGTHEPAKHDMSSLRVIGSVG
                     EPINPEAWMWYREHIGGNSCPVVDTWWQTETGGIMISPLPGATTTKPGSATFPLPGIG
                     AELVDEEGNRVDHGGGYLTLTQPWPGMLRGIWGDPQRY"
     assembly_gap    2551..2562
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2563..>2795
                     /locus_tag="PLV68_15520"
     CDS             <2563..>2795
                     /locus_tag="PLV68_15520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-coenzyme A synthetase"
                     /protein_id="HRE02711.1"
                     /translation="SRFPGRYFAGDGAKLDDDGYLWLLGRVDDVMNVSGHRISTTEVE
                     SALVAHPAVAEAAVVGATDATTGQAIIAYVTLR"
BASE COUNT          473 a          943 c          917 g          450 t
ORIGIN      
        1 gctggcgcgc gtcaaatggc gccatgccgt ccggcagatg acggtcgaag ggaaggaaaa
       61 gggcgtcgag gcggcggagc tgacgaaaag gctgtcgctc gactcgctga cgggacagct
      121 caaccgcgcg gcgttccagg aacgccttca ggcgctcgcc acgcgggacg gcggcgtcgg
      181 gctggtggtg ctgttccaga acagcagcaa cctggccgcg gcctacggca tcgcggtcac
      241 caccaccatg acgatcacca ccctgttgtt ctacgtgctc gccgtgtatc gctggcactg
      301 gtccaaggcc aaggcgctgg ccctggtgat cccattcctc atcgtcgacc tcacgttcct
      361 gggcgcgaac atccccaaga tccccaaggg tggttggttc ccgctgacca tcgcgttctt
      421 gctggtgatc cagatgagca cgtggcggcg cggacgggaa ctggtcgcgg cccgcatcca
      481 ccgcggcgag cgcgacatcg tcgacgtgct cgacgagttc tccgacgtcg tgcgcgtgcc
      541 aggcagcgcg gtgttcatgt tcaaggatct cggcaaggca ccgcccgcgc tggtcaacaa
      601 cctgcgccac aacaaggtgc tgcaccgcac cacgttgatc gtttcggtga ccacggtcga
      661 ggtgccgcgc aacgatccgt cgaagcgcca cgaggtcacc aaggtcgaac cgggtgtgta
      721 tcaggtgctg ctcaagttcg gcttcatgga ggagcccgac gtggtggcgg cgctgtccga
      781 gctgaaggtt cgcggcctcg acttcgaccc gtcgacggcc acgttcttcg tcggccacga
      841 gtcgatcatc gccggcaagg cgccgggcat gtggggcccg cgcgagcacc tgttcgtcat
      901 cctcaaccgc ggcgccgata gcgccagccg cttcttccgc cttcccaacg accgcatctt
      961 cgaagtcggt tcccaagtcg agatctgacc gccgagggcc ggacgatttc cggggcgggt
     1021 cggagggtgt cgctagagtc ggcagcagga ctgtgatccg agcgaacaag agatgggggt
     1081 tggcgatggc cagcgagcag gcacacgatg cgatcgacgc gttgggctgg gagcagcgga
     1141 cgttcccccc gtcggaggag ttcaagcgcg acgcgctggt gcgcggcacg gcgatgtacg
     1201 acgccttggc ggactaccag gggttctggg cgcgccaggc ggccgacctg ttggattggt
     1261 cgaaggactg gcacaccatc tgcgactggg atctgcccga cgcgaagtgg ttcgtcggtg
     1321 gtgagctcaa cgtcagccac aactgcctcg atcgccatgt cgccgctggc cggggcgaca
     1381 aggtagcgat ccacttcgaa ggtgagccgg gcgacacccg caccatcacg tacgccgacc
     1441 tgctggccga ggtgcagcgc tttgccaacg tgttgcgctc gctcggcatc gccaagggcg
     1501 accgggtcaa catctacctc ccgatgatcc ccgaggcggc cgtggcgatg ctggcctgcg
     1561 ctcgcatcgg cgcgccgcac agcgtcgtgt tcggcgggtt ctccgccaac gcgctgaccg
     1621 atcgcatcaa cgacgccgag gccaagctgc tgatcaccgc cgacggtggc taccgccgtg
     1681 gtgaggtgtt cccgctgaag cccaccgccg acgaggcgct cgagaccacg cccagcatcg
     1741 aacacgtcgt ggtcgtgcgc cgtggcggca acaaggtgac gatgcagccc ggtcgcgacc
     1801 tgtggtacca cgagttgatg gccgcagccg atccgctgtg cccggccgag ccgatggaca
     1861 gcgagcagct gttgttcctg ctctacacct cgggcaccac cggcagcccc aagggcatca
     1921 tgcacacgac cggcggatat ctgacccagg tcgcgttcac gcacaagtac gtgttcgatc
     1981 tgcacgtcga caccgacgtg tactggtgca ccgccgacgt cggctgggtc accggccaca
     2041 gctacatcgt ctacggcccg ctggccaacg gcgcgaccca ggtgatgtac gagggcgtcc
     2101 ccaacttccc cggcaacgac cggttctggt cgatcatcga gaagtacaag gtgacgctgt
     2161 tctacaccgc accgaccgcg atccgtacct tcatgaagtg gggcacgcac gagccggcca
     2221 agcacgacat gtcgtcgctg cgggtcatcg gttcggtcgg cgagccgatc aaccccgaag
     2281 cgtggatgtg gtaccgcgag cacatcggtg gcaactcctg ccccgtcgtc gatacgtggt
     2341 ggcagaccga gaccggcggc atcatgatca gcccgctgcc aggggccacc accaccaagc
     2401 cgggctcggc cacctttccg ctgccgggca tcggcgccga actggtcgac gaagagggca
     2461 accgggtcga ccatggcggc ggctacctca ccttgaccca gccgtggccg ggcatgctgc
     2521 gcggcatctg gggtgacccg cagcgctacc nnnnnnnnnn nnggtcgcgc tttcccggcc
     2581 gctacttcgc gggtgacggc gccaagctcg acgacgacgg atacctctgg ctgctcggcc
     2641 gggtcgacga tgtcatgaac gtcagcgggc accgcatctc caccaccgag gtcgagtcgg
     2701 ccctcgtcgc ccatccggcc gttgccgaag cggccgtggt cggtgccacc gacgccacca
     2761 ccggtcaggc gatcatcgcc tacgtcaccc tgcgc
//
LOCUS       DAORNF010000442         2794 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_27239_length_2794_cov_1.979555, whole genome shotgun sequence.
ACCESSION   DAORNF010000442 DAORNF010000000
VERSION     DAORNF010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2794
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27239_length_2794_cov_1.979555"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..722)
                     /locus_tag="PLV68_15525"
     CDS             complement(<1..722)
                     /locus_tag="PLV68_15525"
                     /inference="COORDINATES: protein motif:HMM:NF013673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase modulator"
                     /protein_id="HRE02712.1"
                     /translation="MSQPGSAWEFTVADEAVQAAIDAGARYADARVMHRRAESMGARN
                     GDVDSVDQSADSGLGVRALVGAGWGFFAVADLDTRSAGDAGRRAAEIAAASATVNRAK
                     PAAPGLGLVGSDPAVGSWRSECTIDPFGVSLSVKGDLLVEATQLMREHGADLAEAMYQ
                     AWSTDKWFVSSEGHRVDQRIIECGAGMMATSIGDGETQRRSYPANRGQYRTRGWEFVD
                     ELDLLAHAPRVGSESRALLSAPL"
     gene            828..1181
                     /locus_tag="PLV68_15530"
     CDS             828..1181
                     /locus_tag="PLV68_15530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02713.1"
                     /translation="MKPDAEGHRWLAGARRVLMRRRRTDRAAEQAARLAELILYERLR
                     LMSEFARGLTSTRSSEDVAACVARLVPPLLGLDAAYLAVESPEPTDRWTRKYPLRGHD
                     GATLVVRREQPEWRR"
     gene            1178..2020
                     /locus_tag="PLV68_15535"
     CDS             1178..2020
                     /locus_tag="PLV68_15535"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HRE02714.1"
                     /translation="MILTVVDLVTGALTRSRLHDHEQVVLRSFQESLFTPPPIVPGLD
                     IAAGYRPAIEAVGMGGDWYSIVDTPDAVYAVIGDIAGHGPGAVAVMAEAKTIMRHLLT
                     NGSTIEDTLQHADRALTRRGVYASAAVVRIDKATGLLRYANAGHPPALLFHRDDAGAS
                     TVTALATVHRPWLGVGVALPTATTGDCSQQVSEHRLGAGDVLLLYTDGLVEERRETLE
                     TSIRQQLYALDVDRPAAVIVDHLLTNRQTHRTPRSVDDDVAVVVIARTGDTAVTEPVH
                     RPQP"
     gene            complement(2038..2757)
                     /locus_tag="PLV68_15540"
     CDS             complement(2038..2757)
                     /locus_tag="PLV68_15540"
                     /inference="COORDINATES: protein motif:HMM:NF016488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HRE02715.1"
                     /translation="MTDQLDVAIDVAIDALDDIAEPVIGPVSEPDDPAAQERRFVEDF
                     TRMSMLHKFAIDEVRTKINILREEFVHLHDYNPIEHVNWRLKTLDSVVAKARRRGCEL
                     TVASVRDAVRDIAGVRVVCSFVADVYHLFEVLTAQADVIVVEVDDYITEPKPNGYRSL
                     HATIEVPVFLSTGAEYVYVELQLRTVAMDFWASLEHKIYYKYDREVPDSLLGELKEAA
                     EIAAALDGRMQRLHREVASLD"
BASE COUNT          454 a         1017 c          883 g          440 t
ORIGIN      
        1 agcggtgccg acagcagggc ccgcgactcg ctaccgaccc gtggcgcatg ggcgagcaga
       61 tcgagctcgt cgacgaactc ccacccccgc gtgcggtact gcccacggtt ggccggatac
      121 gagcggcgct gcgtctcgcc gtctccgatc gacgtcgcca tcatgcccgc cccgcactcg
      181 atgatccgct ggtcgactcg gtggccctcg ctggacacga accacttgtc ggtcgaccac
      241 gcctgataca tcgcctcggc caggtcggcg ccgtgctcgc gcatcagctg agtcgcttcg
      301 acgagcaggt cacccttgac cgacagcgag acaccgaacg ggtcgatcgt gcactcgctg
      361 cgccacgacc cgacggccgg atcggacccg accaaaccca gtcccggcgc tgcgggcttg
      421 gcccgattga ccgtcgcgct ggccgcagcg atctcggccg cccttcggcc ggcgtcaccc
      481 gccgaacggg tgtcgagatc ggcgacggca aagaaccccc acccggcccc gaccagcgcc
      541 cgcactccca gaccactgtc ggccgactga tcaacgctgt caacgtcgcc gttgcgggcg
      601 cccatcgact cggcccggcg atgcatcacc cgtgcgtccg cgtagcgagc accagcgtcg
      661 atcgccgcct gcacggcttc gtccgcgacg gtgaactccc acgcagaccc gggttggctc
      721 atgtcgccga ccatacaacc atccttgcca cccggtaccc gatcacggtc acgcagaccc
      781 gagaacgaca ccacctttcg tccacggcgt ggctatggtc cgagatagtg aagccggatg
      841 cagaagggca ccgctggttg gccggcgcac ggcgtgtcct catgcggcgg cgccggaccg
      901 accgcgccgc cgagcaggcg gcacgcctgg ccgagctgat cctgtacgag cgcctccggc
      961 tcatgagcga gttcgcccgc gggctgacct ccacccggtc ctccgaagac gtcgccgcat
     1021 gcgtcgcacg cctcgtacca ccgctgctcg gtctcgacgc cgcttatctc gctgtggaat
     1081 cgcccgagcc gaccgatcga tggacgagga agtatccgct ccggggtcac gacggcgcaa
     1141 cgctggtcgt acgccgcgag cagccggagt ggagacggtg atcttgacgg ttgtcgacct
     1201 cgtcaccggt gccctcaccc gctcgcgact gcacgaccac gaacaggtgg tgctgcgcag
     1261 cttccaagag tcgctgttca ccccgccacc catcgtgccc gggctcgata tcgcggccgg
     1321 ctaccgcccc gccatcgaag cggtcggcat gggcggtgac tggtattcga tcgtcgacac
     1381 gcccgacgcc gtctacgcgg tgatcggtga catcgccgga cacggccctg gtgcggtcgc
     1441 ggtgatggcg gaagccaaga cgatcatgcg ccacctgttg accaacggca gcaccatcga
     1501 agacacactg cagcacgctg accgggcgct gacccgtcgc ggcgtctatg cgtcggcggc
     1561 ggtcgtgcgg atcgacaagg ccacgggctt gctgcgctac gccaacgcgg gtcatccacc
     1621 ggcgctgctg ttccaccgcg acgatgcggg tgccagcacg gtgaccgcgc tcgccacggt
     1681 gcaccgcccg tggctcggag tcggggtcgc cctccccacg gcgaccaccg gcgattgcag
     1741 ccagcaggtt tccgagcacc ggctcggcgc cggcgacgtg ttgctgctct acaccgacgg
     1801 cctggtggaa gagcgccgcg aaaccctgga gaccagcatc cgccaacagc tgtacgccct
     1861 cgacgtcgac cgccccgcgg cagtgatcgt cgaccacctg ctgacgaacc gtcagacgca
     1921 ccgcacccca cgctcggtcg acgacgacgt ggccgtggtc gtcatcgccc gcaccggcga
     1981 cacagcggtg acggagccgg ttcatcgacc acaaccgtga tgatgcgccg gtcgatgtca
     2041 gtcgagtgag gcgacctcgc ggtggaggcg ttgcatccgc ccgtcgagcg cagccgcgat
     2101 ctcggccgct tccttcaact cgcccagcag tgagtcggga acctcgcggt cgtacttgta
     2161 gtagatcttg tgctccaggc tggcccagaa gtccatcgcc acggttcgca actgcaactc
     2221 gacgtagacg tactcggctc ccgtcgagag gaagaccggc acctcgatgg tcgcgtgcag
     2281 gctgcggtag ccgttgggct tgggctcggt gatgtagtcg tcaacttcga ccacgatcac
     2341 gtcggcctgc gccgtcagca cctcgaacag gtgatacacg tcggccacga agctgcacac
     2401 cacccgcacc ccggcaatgt cgcggaccgc atcgcgcacc gatgccacgg tgagctcaca
     2461 gccgcggcgg cgggccttgg cgacgacgct gtccagcgtc ttcagccgcc aattgacgtg
     2521 ttcgatcggg ttgtagtcgt gcaggtggac gaactcctcg cgcaggatgt tgatcttcgt
     2581 ccgtacctcg tcgatcgcga acttgtgcaa catcgacatg cgggtgaagt cctcgacgaa
     2641 acgtcgctcc tgggcggctg gatcgtcggg ctcgctcacg ggcccgatca ccggctcggc
     2701 gatgtcgtcc agcgcatcga tcgcgacgtc gatcgcgacg tcgagctggt cggtcatggg
     2761 ccgaccttac cggcccctgg cccgactggt ctcg
//
LOCUS       DAORNF010000443         2787 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_27336_length_2787_cov_1.665447, whole genome shotgun sequence.
ACCESSION   DAORNF010000443 DAORNF010000000
VERSION     DAORNF010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2787
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27336_length_2787_cov_1.665447"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            244..>903
                     /locus_tag="PLV68_15545"
     CDS             244..>903
                     /locus_tag="PLV68_15545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02716.1"
                     /translation="MDLSRRAAFESHRLIGWIFWDPAAIANYAALGVPDGVGYYIVSR
                     AAPLAAAGHQAVTAAYGSIHAGFVQATIELSEAHTTCAAAADARDRAVTAGLAAFVPE
                     ITAELATFAQPLWDAADSLDSAGRVLFAAHRQWPRPTDDDVLSAWLALNCIREWRGDT
                     HWAIQIADGLSPVAAGVLDGAWRAYENDWLARSRGADDAALARAMGELAERGFVTDGR
                     VN"
     assembly_gap    904..924
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <925..1116
                     /locus_tag="PLV68_15550"
     CDS             <925..1116
                     /locus_tag="PLV68_15550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02717.1"
                     /translation="RHRQALEDRLDRLTVAPWQHLGAERTEALIALIEPVGQRLVERI
                     NETAGPNWMPAARTRRASL"
     gene            1230..2336
                     /gene="nspC"
                     /locus_tag="PLV68_15555"
     CDS             1230..2336
                     /gene="nspC"
                     /locus_tag="PLV68_15555"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: protein motif:HMM:TIGR01047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0008216 - spermidine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HRE02718.1"
                     /translation="MSELRTPYYLIDEARLVQNLEVVRRVRESSGAKSLLALKCFSTW
                     SVFPLMREYMDGTTSSSLFEARLGHERFGGETHAYSVAYSEADVAAVATFADKVIFNS
                     VSQLERFAPLVAHLPVGLRVNPGVSHSHFDLADPARRHSRLGELDIDVVRSVLDRISG
                     VMFHFNCENDDFASFATLLDAIGQRYADVLARLDWVSLGGGIAFTAPGYPVDELCALL
                     TGFAASHGVQVYLEPGEAAITHAGELVTTVLDVVHNEIDVAIVDASIEAHMLDHLIYA
                     TTPRLNAPGPGAHPVIIAGRTCLAGDAFGEHRLAARLQVGDEVRIADAAGYTMVKKSW
                     FNGVAMPSIAVRRLDGTIDVVRQFGYDDFESSLS"
     gene            2395..>2787
                     /locus_tag="PLV68_15560"
     CDS             2395..>2787
                     /locus_tag="PLV68_15560"
                     /inference="COORDINATES: protein motif:HMM:NF015400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HRE02719.1"
                     /translation="MKQNVLIIGAGGVAHVAAHAAAQRNDVLGNICIASRTVSKCDEI
                     IASVHRKGHVKDASKLLYSRQIDALDIDATAALIRDTDSEIVVNLGSAFLNMSILEAC
                     LRTGAAYIDTAIHEEPDAVCENPPWYANY"
BASE COUNT          437 a          910 c          947 g          472 t
ORIGIN      
        1 ccgtccggag agcgcacccg ggtgtacttc ccgtccgtgc gggggacgga cgttgatgag
       61 tatgtgctgg tcgcggacga ttcatcagcg gaagcgcggt gggcggggtt ggtgggtggt
      121 ggcaatgtgt cagtagctct gacagaatat ttgggttgtg catggacaga gtgcacgctg
      181 gatcggatga ggtgtgaggg ggagagtgtc gtgcacgacc gtcggaacga cgaagtggat
      241 tgggtggatt tgtcgcgccg ggcggcgttc gagtcacacc ggctgatcgg gtggatcttc
      301 tgggatccgg cggcgatcgc caactacgcc gcgctcggcg tgcccgacgg tgtgggctac
      361 tacatcgtca gccgggcggc tccgctcgcc gccgctggac atcaggccgt gaccgccgcg
      421 tacggctcga tccatgccgg gttcgtgcag gccacgatcg agctgagtga ggcgcacacc
      481 acctgtgcgg ccgcggcgga cgcccgcgac cgggccgtca cggcagggct cgccgcgttc
      541 gtaccggaga tcaccgctga actggccacc ttcgcccagc cgctgtggga tgccgccgac
      601 tcgctcgact cggcggggcg ggtgctgttc gcggctcatc ggcagtggcc gcgtccgacc
      661 gacgacgatg tgttgtcggc gtggctggcc ctgaactgca tccgtgagtg gcgcggcgac
      721 acgcattggg cgatccagat cgccgacggg ctctcaccgg ttgccgcggg cgtgctcgac
      781 ggcgcatggc gcgcctacga gaacgactgg ctagctcgca gccgcggtgc cgacgacgct
      841 gcgctcgcac gggcgatggg cgagctcgcc gagcgcgggt tcgtcaccga cgggcgggtc
      901 aacnnnnnnn nnnnnnnnnn nnnncgccat cgccaggcct tggaggatcg cctcgaccgg
      961 ttgacggtcg cgccatggca gcacctcggg gccgagcgca ccgaggcgct gatcgcgttg
     1021 atcgagccgg tcggacagcg actggtcgag cggatcaacg agacggctgg cccgaactgg
     1081 atgcctgcgg cccgcacccg tcgcgcctcg ctgtagacgc ggcatccggc accgaccgcg
     1141 ccctcgcggt agtggggcga gtttgatggg accggccact ggatgaaggg gccgcatcgc
     1201 cctccggcac cgttacgctg ggaggccgtt tgagcgagct gcgtaccccc tattacctca
     1261 tcgacgaggc gcgacttgtc cagaacctgg aggtcgtccg gcgcgtccgt gaatcctcag
     1321 gtgctaagtc gctgctggcg ttgaagtgct tctcgacgtg gtcggtgttc ccgctgatgc
     1381 gcgagtacat ggacggtacg acgagcagtt cactgttcga agcccgcctg ggtcacgaac
     1441 gcttcggcgg cgagacccac gcctacagcg tcgcctacag cgaggccgac gtcgccgccg
     1501 tggccacctt cgccgacaag gtgatcttca actcggtcag ccagctcgaa cggtttgccc
     1561 cactggtcgc ccacttgccg gtcggtctac gggtcaaccc gggtgtcagc cactcgcact
     1621 tcgacctggc cgatccggcg cggcgtcact ctcgcctcgg tgagctcgac atcgacgtcg
     1681 tgcgcagtgt gctcgaccgg atctccggcg tgatgttcca cttcaactgc gagaacgacg
     1741 acttcgcgtc gttcgccacg ctgctcgacg cgatcggtca gcgctacgcc gatgtgctcg
     1801 cccggctcga ctgggtcagc ctcggcgggg ggatcgcgtt caccgcgccg ggctatccgg
     1861 tcgacgagct gtgcgcgctc ctgaccggct tcgctgccag tcacggggtg caggtgtacc
     1921 tcgaaccggg agaggccgcg atcacgcatg ccggtgagct ggtcaccacc gtgctcgacg
     1981 tggtgcacaa cgagatcgac gtcgcgatcg tcgacgcgtc gatcgaagcg cacatgctcg
     2041 accacctgat ctacgccacc acgccccggc tcaacgcgcc cggtccgggc gcgcatccgg
     2101 tgatcatcgc ggggcgcacc tgcctggccg gcgacgcgtt cggtgaacat cgtctggctg
     2161 cacggttgca agtcggcgac gaggttcgca tcgccgacgc ggcgggctac accatggtca
     2221 agaagtcgtg gttcaacggc gtggcgatgc cctcgatcgc ggttcgtcgt ctcgacggca
     2281 ccatcgacgt cgtgcgccag ttcggctacg acgacttcga atcgagtctg tcgtgagcgt
     2341 ccggctcacg cagactgtca gcacatcaac cagttccgaa cggaaggatc agcgatgaag
     2401 cagaacgtgt tgatcatcgg cgccggtggc gtcgcccatg tggccgccca cgcagcggct
     2461 caacgcaacg atgtgctcgg caacatctgc atcgcgtcgc gcacggtcag caagtgcgac
     2521 gagatcatcg ccagcgtgca ccgcaagggg cacgtcaagg acgcgtcgaa gctgctgtac
     2581 tcgcgccaga tcgacgcgct cgacatcgac gccaccgcgg ccctgatccg cgacaccgat
     2641 tcggagatcg tggtcaacct cggttcggcc ttcctcaaca tgtcgatcct cgaagcgtgc
     2701 ctgcgcaccg gtgcggccta catcgacacc gcgatccacg aagaaccgga cgcggtgtgc
     2761 gagaacccgc cgtggtacgc caactac
//
LOCUS       DAORNF010000444         2785 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_27370_length_2785_cov_1.850549, whole genome shotgun sequence.
ACCESSION   DAORNF010000444 DAORNF010000000
VERSION     DAORNF010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2785
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27370_length_2785_cov_1.850549"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>414)
                     /locus_tag="PLV68_15565"
     CDS             complement(<1..>414)
                     /locus_tag="PLV68_15565"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HRE02720.1"
                     /translation="GRVAGWAAANWLLDAAALWVFLRAFGGSLSIDGLLVSFGLANIA
                     AVVPITPGGLGIVEGVYIPTLVWFGLTRGSATLGVASYRLAQFFFPILLGAILYLTLR
                     VGRWRIGGPVELKRLRDEAKDSTAELETPIDFYERV"
     assembly_gap    415..448
                     /estimated_length=34
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<449..>818)
                     /locus_tag="PLV68_15570"
     CDS             complement(<449..>818)
                     /locus_tag="PLV68_15570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02721.1"
                     /translation="KALGSVVPGGSAASSALGYRLLTLSGVPGTDAGFALGTAGLGSA
                     VVLNLIFWLGLIVSIPLNGVNSAYVSAAIAGVLIMGLAATIVVGLMQGQARAEKVLRW
                     VARRLRMNEDNAGKAVRHIGG"
     assembly_gap    819..879
                     /estimated_length=61
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<880..1155)
                     /locus_tag="PLV68_15575"
     CDS             complement(<880..1155)
                     /locus_tag="PLV68_15575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02722.1"
                     /translation="MSEVSDEATNSAAPTSARSQTPDRPPKPFRPVRKALNLFAFAAI
                     IWFFVLPLIPGFRKAADELSKVNPALLAVGLGLEVAALFAYSLMTRAA"
     gene            complement(1435..1788)
                     /locus_tag="PLV68_15580"
     CDS             complement(1435..1788)
                     /locus_tag="PLV68_15580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02723.1"
                     /translation="MGPLGLGSLIVKPRRHVTFVGDLSDDEVSELGPLLRTASAIAAE
                     LVGAEQVYNCLWSHAEGKPGHLHYVVQPVTAEAMNHHGTYGPALQMAMFTAGDRPSTA
                     EIEAIAARARAAFAN"
     gene            complement(1946..2266)
                     /locus_tag="PLV68_15585"
     CDS             complement(1946..2266)
                     /locus_tag="PLV68_15585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02724.1"
                     /translation="MPGNILFGLLGAAVHGVVSMIKAERRERRRRDEEIMRVEDDYRS
                     GRLRLRSSRTARQPASRPRPAETATERPVGRATISGDGVVIRSSVSEPTATSPAPDPP
                     AGPR"
BASE COUNT          459 a          948 c          922 g          361 t
ORIGIN      
        1 acgcgctcgt agaagtcgat cggggtctcc aactcggcgg tggaatcctt cgcctcgtcg
       61 cgcagacgtt tcaactcgac cgggccaccg atgcgccacc gcccgacgcg caaggtgagg
      121 tacaggatcg cgccgagcag gatcgggaag aagaactgcg cgagccggta cgacgcgacg
      181 ccgagggtgg ccgagccgcg ggtgaggccg aaccacacca acgtcggaat gtagacaccc
      241 tcgacgatgc cgaggccacc cggcgtgatg ggcacgacag cggcgatgtt ggccagcccg
      301 aagctgacca acaaaccgtc gatgctgagc gagccgccga aggcgcggag gaacacccac
      361 aacgcggccg cgtcgagcag ccagttcgcc gccgcccacc cggcgacgcg accgnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnncc gccgatgtgg cgcaccgcct tgccggcgtt
      481 gtcctcgttc atccgcagtc gccgcgccac ccagcgcaac accttttccg cccgggcctg
      541 gccctgcatc agcccgacca cgatcgtggc cgcgagcccc atgatcaaca cgcccgcgat
      601 cgcagccgac acgtaggccg agttgacccc gttgagcggg atcgacacga tcaacccgag
      661 ccagaagatc aggttcaaca ccactgcgga cccaaggcct gcggtaccga gggcgaaccc
      721 agcgtcggtg ccgggcacgc cggaaagcgt cagcaagcgg taaccgagtg ccgagctggc
      781 cgcactgccg ccgggcacga cgctgccgag tgccttggnn nnnnnnnnnn nnnnnnnnnn
      841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc gcggcccggg tcatcagcga
      901 gtaggcgaac agtgccgcga cctcgagccc gagcccgacg gcgagcagcg ccgggttgac
      961 cttgctcagc tcgtccgcgg ccttgcggaa gccggggatc agcggcagga cgaagaacca
     1021 gatgatcgcc gcgaaggcga aaaggttgag cgccttgcgg accggccgga atggcttcgg
     1081 cggtcggtcc ggggtctgag accgagccga ggtcggggca gcgctgttcg tcgcctcgtc
     1141 cgacacctcg ctcacccgct ccttgctagc acaccgcccc gaacccaact cgcccgcaag
     1201 cccctccggc acccccgccg ctcctccgcg gcagctgaat tgcttgcccc ggtgaatctg
     1261 gcctccgcgg ggcgaggact ccccgcagcc cggcaaaccc gtactccgcg gaagccaaac
     1321 tgcccgcccc ggtgaatcca ccgtccgcgg aacgaggatc acccggagac cggcaactcc
     1381 acactccggc gcagccggat gtgctgccgg tgcgcacgct ggaagcgtcg gagttcagtt
     1441 ggcgaaggcg gcgcgggcgc gggcggcgat cgcttcgatt tcggcggtcg acgggcggtc
     1501 gcccgcggtg aacatcgcca tctggagcgc cgggccgtag gtgccgtggt ggttcatcgc
     1561 ctcggcggtc acgggttgga cgacgtagtg caggtgtccg ggcttgcctt cggcgtgcga
     1621 ccagaggcag ttgtacacct gctcggcgcc gacgagttcg gccgcgatgg ccgaggccgt
     1681 gcgcagcaag gggccgagct cggacacctc gtcgtcggac aagtcgccga cgaaggtgac
     1741 gtggcggcgc ggcttgacga tcagacttcc cagcccgagc ggccccacgc agtgttcgac
     1801 cagccagtgc tccgtggcgt ggatccgacc gccgggcaag ggttcggttc cgtcagccaa
     1861 gtcgcaggca aagcagcgtc gcatgccacg agtcttgccg ctcgcccgcc gcccccaccg
     1921 tcgccccgct cgactcgagc gcgactcagc ggggcccggc cggcggatca ggggcaggag
     1981 aagttgctgt cggctcggag acggatgagc ggatgacgac gccgtcgccg gagatggtgg
     2041 cccgccccac tggacgctcg gtcgccgtct cggcagggcg cgggcgagac gcaggctgtc
     2101 tcgcggtccg cgacgaacgc aggcgcaatc ggccggagcg gtagtcgtct tcgacgcgca
     2161 tgatctcctc gtcgcgtcgg cggcgctcgc ggcgctcggc cttgatcatc gagacgacgc
     2221 cgtgcacggc agcaccgagc agaccgaaga ggatgttgcc tggcacgcca ccaagagtgc
     2281 tccaccgcat gccaccacgg aaaacaaaac tcgagccggc acgcgatccg tggcagcgat
     2341 tcacactcag cgacgcgatc ccgcctcgac cccgcccgcc gtgtgttcag tgaacactcc
     2401 cagaaggggt gaatcgaacg cacttgggcc gtcggcctcg gacggtgttc agttgacccc
     2461 tgccgctggg gtcgatcgaa cgcacttggg ccgtcggcct cggacggtgt tcagttgacc
     2521 cctgccgctg gggtcgatcg aacacacctg acccgccgcc aagggggcag cttccggaca
     2581 gcgcggtgtg ctgacgggcg tcgagccgcg agactggggg catgacggaa gctgcgtccg
     2641 atccgatcac cgatcaaccg tcactcagtg gctcctcgac gaccggcggc accgcaccga
     2701 agagctccgg cctcggcggt ggcgaggccg ttgcagatgc aacgaccgac gagctgtggc
     2761 gggaccgcct gtcgcggtgg ctggc
//
LOCUS       DAORNF010000445         2784 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_27382_length_2784_cov_2.057530, whole genome shotgun sequence.
ACCESSION   DAORNF010000445 DAORNF010000000
VERSION     DAORNF010000445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2784
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27382_length_2784_cov_2.057530"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(80..>306)
                     /locus_tag="PLV68_15590"
     CDS             complement(80..>306)
                     /locus_tag="PLV68_15590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02725.1"
                     /translation="GYNDPNMDAALEALRLAPDVTAEKEALTDLQAAWTDTVPHIVLG
                     AAVNFVGYSGIVGLVPSSDTIILYDQARAG"
     assembly_gap    307..413
                     /estimated_length=107
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<414..783)
                     /locus_tag="PLV68_15595"
     CDS             complement(<414..783)
                     /locus_tag="PLV68_15595"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRE02726.1"
                     /translation="MNSGAKGSTPPTSDVRIRRAVAAAIDVDIINDRAEDGKGKPTTL
                     AIAPGSILYQDLPGPKYDLDLAKQLVEEVKAEGWDGSIRFQCSPDPLQNEQALTAAAM
                     LTAAGFTVDMTTTATIGDMVT"
     assembly_gap    805..972
                     /estimated_length=168
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<973..1750)
                     /locus_tag="PLV68_15600"
     CDS             complement(<973..1750)
                     /locus_tag="PLV68_15600"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRE02727.1"
                     /translation="MVRSRVRRRSAATSLALVAALLAACGSDEKASPTSSTAAGGSST
                     STASSSPTSTGPSSSSPSSTPAGNSACSTTGEGGEITVAAYSFTQSLDPTVAGSNGLL
                     GASELAAIYDTLMRFNTETGAFEPRVAESLEPNSDFTEWTLTLRDGVTFGNGDPLTAA
                     DVVASIERHAAEESKSTYKSLVAVISGMQAVDDLTVTFTLAQPWPKFPYVLSLGPGMI
                     TNPRIADERGGDLVSNPEGAGVGPFEFSRFAPNEEVVLTPR"
     gene            complement(1756..2169)
                     /locus_tag="PLV68_15605"
     CDS             complement(1756..2169)
                     /locus_tag="PLV68_15605"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRE02728.1"
                     /translation="MTQLWALTDGGNDWGDHDPTVGLPLSTDLAAGGSRWVSIEFGPH
                     SAAEAHVTETVDLLYVVAGAITLELDGSVVELGQGDVLVQQGTRHAWRNDHDEPCHVL
                     VHVISSRASASNPPMAEAVSTNATAVPTNECQQGV"
     gene            2379..>2784
                     /locus_tag="PLV68_15610"
     CDS             2379..>2784
                     /locus_tag="PLV68_15610"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRE02729.1"
                     /translation="MGIGVSEGLEPTSNDFAGQVVLVTGAARGQGASIAQRFAVAGAL
                     VVVTDVLDGGGATTAASIGGDRAVAVELDVTAEDRWQHAVETALATFGRLDVLINNAG
                     ILHSEPLLTTSTDAFRRVLDVNLLGAFLGIRTA"
BASE COUNT          379 a          833 c          851 g          446 t
ORIGIN      
        1 gtgcccccgg gatcccaccg gcagacgcgc aggtgcgcgg ccccgcaggg ggccgcgcac
       61 cgacgggggg aagggtcggt cagcccgcac gcgcctggtc gtacaagatg atcgtgtccg
      121 acgacggcac caggccgacg atccccgagt agccgacgaa gttcaccgcg gcgccgagca
      181 cgatgtgcgg gacggtgtcg gtccacgcgg cctgcaggtc ggtgagcgcc tccttctcgg
      241 ccgtcacgtc gggggccagt cgcagtgctt cgagtgcagc atccatgttc ggatcgttat
      301 agccgtnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntggtcac
      421 catgtcaccg atggtcgccg tggtggtcat gtcgacggtg aagccggccg cggtcagcat
      481 cgccgccgcg gtcagcgcct gctcgttctg caacgggtcg ggcgagcact ggaagcggat
      541 cgagccgtcc cacccttcgg ccttgacctc ctctaccagt tgcttggcga gatcgaggtc
      601 gtacttcggt ccgggcagat cctggtacag gatcgagccg ggtgcgatcg ccaacgtcgt
      661 cggcttgccc ttcccgtcct cggcgcggtc gttgatgatg tcgacatcga tggccgcggc
      721 cacggcgcga cggatgcgga cgtcggacgt cgggggcgtg ctgcccttgg cgccgctgtt
      781 catcgcgaac agccagcccg agctnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      961 nnnnnnnnnn nncacgtggc gtcagcacga cctcttcgtt gggcgcgaag cgggagaact
     1021 caaacggccc gacgcccgcc ccttcggggt tgctgaccag gtcgccgccg cgctcatcgg
     1081 cgatgcgcgg gttggtgatc atgcccggcc ccagcgagag tacgtagggg aactttggcc
     1141 acggctgggc tagggtgaag gtcaccgtca gatcgtcgac ggcctgcatc ccggagatca
     1201 ccgcgaccag gctcttgtag gtcgacttgc tctcctcggc tgcatgtcgc tcgatgctcg
     1261 cgacgacgtc ggccgcggtg aggggatcgc cgttgccgaa cgtcacgcca tcgcgcagcg
     1321 tgagcgtcca ttcggtgaag tcgctgttcg gttcgaggga ctcggccacg cgcggttcga
     1381 acgcgccggt ctcggtgttg aagcgcatca gcgtgtcgta gatcgcagcc agctcgctcg
     1441 cgccgagcag gccgttggaa cctgcgaccg tcgggtcgag gctctgcgtg aagctgtacg
     1501 cggcgaccgt gatctcacct ccctcccccg tggtgctgca cgcgctgtta cccgcggggg
     1561 tggacgaggg gctcgagctc gacggacccg tactggtcgg ggacgacgat gcggtgctcg
     1621 tagacgagcc acctgctgca gtgctcgatg ttggcgacgc cttctcgtcg ctgccacatg
     1681 cggcgagcaa cgcggcgacg agggcgaggc tggtcgccgc agatcgacgg cggactctcg
     1741 atcggaccat tgtgttcaca ctccttgttg acattcgttg gttggcacgg ctgttgcgtt
     1801 ggttgatacg gcttccgcca tgggcgggtt cgatgcactc gctcgcgaac tgatgacgtg
     1861 caccagcacg tggcacggct cgtcgtggtc gttgcgccag gcgtggcggg tgccctgttg
     1921 caccagcaca tccccctgcc cgagctcgac gaccgagccg tccagttcga gcgtgatcgc
     1981 accggccacg acgtagagca gatcgacggt ctcggtgacg tgcgcttcgg ccgcgctgtg
     2041 cggaccgaac tcgatcgaca cccaccgtga gccaccagcg gcgaggtcgg tggacagggg
     2101 gaggccgacc gtggggtcgt gatcgcccca gtcgttgccc ccatccgtca acgcccagag
     2161 ctgcgtcacc gtgttcgttc caaagacgaa cgacgtcggt ggcgccccat cggcgacgac
     2221 tctcgacttg ccatcgcgat cgttcgcggt gacgacccta cgcatacccg taaccccccg
     2281 attcgggtgc attctcctga ggcaccgtcc tcgatttcga accatacaag ttgttggctg
     2341 tttagtcaac caacagactt tgagctaccg tgaagcgcat ggggattggg gtatccgagg
     2401 ggcttgagcc cacatcgaac gactttgcgg ggcaggtcgt gctcgtcacc ggtgccgctc
     2461 gcggacaagg ggccagcatc gcccagcgct tcgcagtcgc cggtgcgctg gtcgtcgtga
     2521 ccgacgtgct cgacggcggg ggcgcgacga cggcagcatc gatcggaggc gatcgtgccg
     2581 tggccgtcga gctcgatgtc accgccgagg accgatggca acacgcggtc gagacggcac
     2641 tcgccacctt cggccgtctc gacgtgctga tcaacaacgc cggcatcctc cacagcgagc
     2701 cgctgctcac gacgagcacc gatgccttcc gccgcgtcct cgacgtcaac ctgctcgggg
     2761 cgttcctcgg catccgcacc gccg
//
LOCUS       DAORNF010000446         2781 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_27420_length_2781_cov_1.520176, whole genome shotgun sequence.
ACCESSION   DAORNF010000446 DAORNF010000000
VERSION     DAORNF010000446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2781
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27420_length_2781_cov_1.520176"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>349
                     /locus_tag="PLV68_15615"
     CDS             <1..>349
                     /locus_tag="PLV68_15615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02730.1"
                     /translation="VGTLLGNAVTTAWGGAGLPDGTIRVHCRGSAGQSFGAFLPAGIE
                     LRLEGDGNDYVGKGLSGGRIVIHPDRTSTLVAEHNVIAGNVIGYGATGGEIYLRGVVG
                     ERFCVRNSGAVAVV"
     assembly_gap    350..403
                     /estimated_length=54
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <404..760
                     /locus_tag="PLV68_15620"
     CDS             <404..760
                     /locus_tag="PLV68_15620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02731.1"
                     /translation="RVAVLGPTGRNFAAGMSGGVAYVYDPQADFAARVNPELVALEEL
                     DPADEAELVAMVTSHRDHTGSTVAAAILADWDVARASFRKVMPRDYKRVLAVMQAAAA
                     EGLDEAAAAARVMETV"
     assembly_gap    885..982
                     /estimated_length=98
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <983..2204
                     /locus_tag="PLV68_15625"
     CDS             <983..2204
                     /locus_tag="PLV68_15625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HRE02732.1"
                     /translation="DAIDRLHATNNFPEFTGRLCPAPCEAACVLGINADPVTIKQVEV
                     EIIERAFREGWVQPQPPSVKTGKRVAVIGSGPSGLAAAQQLTRAGHDVVVLERADRIG
                     GLLRYGIPEFKMEKRFLDRRVEQMEAEGTEFRTNATVGDGIDIDVLLATNDAVVMACG
                     ATAWRDLPIPGRELRGIYQAMEFLPLANRVQEGDMEISPINAAGKHVVIIGGGDTGAD
                     CLGTSHRHGAASVTQLEIMPEPPTSRSANNPWPQWSLILRTSSAHEEGGERVYSVNTE
                     RFLGDDDGNVRALLLHEVEMVDGRFTKIEGSDRELPADLVFLAMGFLGPEHGGWLGQL
                     GVGYDERGNVARDANFMSSVPGVFVAGDMGRGQSLIVWAIAEGRSCAAGVDRYLMGET
                     DLPAPIAPTARPLQ"
     gene            2308..>2781
                     /locus_tag="PLV68_15630"
     CDS             2308..>2781
                     /locus_tag="PLV68_15630"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HRE02733.1"
                     /translation="MTFRLPAVLVGAAALVGLTACGSEAGTGASSQSTRVEISQPVYV
                     TAPPVTTAATIAGQDQTVGGIVNEEQQYTVVFGDYLIGIAKKHCISLDTLVAYNNWPE
                     GNKRAGNPNDVVRIPSGSCAPGTDLSAAATGSGQTGQTGGATAPTPSSAAPTTEYT"
BASE COUNT          440 a          880 c          933 g          376 t
ORIGIN      
        1 ggtgggcacg ctgctcggca acgccgtcac cactgcctgg ggcggcgccg gtctgcccga
       61 cggcacgatc cgtgtccact gccgcggttc ggccgggcag agcttcggtg cgttcctgcc
      121 ggcaggcatc gaactgcgcc tggaaggtga cggcaacgac tacgtcggca agggcctgag
      181 cggtgggcgg atcgtgatcc accccgatcg cacctcgacg ttggtggccg agcacaacgt
      241 gatcgcgggc aacgtcatcg gatacggcgc aaccggtggc gagatctacc tgcgcggcgt
      301 ggtgggcgag cgattctgcg tgcgcaactc cggcgccgtc gcggtggtcn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncgcgtcg ccgtgctcgg
      421 ccccacgggg cgcaacttcg cggctggcat gagcgggggg gtggcctacg tctacgaccc
      481 gcaggccgac ttcgcggctc gggtcaaccc ggagctggtc gcgttggagg agctcgaccc
      541 ggccgacgaa gccgagttgg tcgcgatggt gaccagccat cgcgaccaca ccggctcgac
      601 ggtggctgca gcgatcctcg ccgattggga cgtcgcccgg gcgtcgttcc gcaaggtgat
      661 gccccgtgac tacaagcggg tgctcgcggt gatgcaggcg gctgcggccg aaggtctgga
      721 cgaagcggca gctgccgcgc gggtgatgga gacggtctga tgggtgaagc gacagggttc
      781 ttgcaatggc cgcgggagac gccgacgcgg cgaccggtgc cggtgcggtt gcgtgactgg
      841 aaggaggtct acgagccgtt cgcgctcgac aagatcacca tccannnnnn nnnnnnnnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      961 nnnnnnnnnn nnnnnnnnnn nncgacgcga tcgaccggct gcacgccacc aacaacttcc
     1021 ccgagttcac gggccggctg tgcccggcac cgtgcgaggc ggcctgcgtg ttggggatca
     1081 acgccgaccc ggtcacgatc aagcaggtcg aggtcgagat catcgaacgc gccttccgtg
     1141 agggttgggt gcaaccacag ccgcccagcg tcaagaccgg caagcgcgtg gctgtcatcg
     1201 gcagtggccc gtcgggactc gccgccgcgc agcagctcac ccgcgccggt cacgacgtcg
     1261 tcgtgctcga acgggccgac cgcatcggcg gcctgctgcg ctacggcatc cccgagttca
     1321 agatggagaa gcggttcctc gaccggcgcg tcgaacagat ggaggccgaa ggcaccgagt
     1381 tccgcaccaa cgccacggtc ggtgacggca tcgacatcga cgtgttgctc gccaccaacg
     1441 acgcggtcgt gatggcgtgc ggcgcgacgg cctggcgcga cctgcccatc cccggacgcg
     1501 agctgcgcgg catctatcag gcgatggagt tcttgccgct ggccaaccgc gtgcaggaag
     1561 gcgacatgga gatctcaccg atcaacgcgg caggcaagca cgtggtgatc atcggcggcg
     1621 gcgacaccgg cgccgactgc ctcgggacgt cgcaccgcca cggggcggcc tcggtgaccc
     1681 agttggagat catgcccgag ccaccgacgt cgcggtcggc caacaacccg tggccgcagt
     1741 ggagcctgat cctgcgcacc tcgtcggccc acgaggaagg aggagagcgg gtctacagcg
     1801 tcaacaccga acgcttcctc ggcgacgacg acggcaacgt ccgcgccctg ctgttgcacg
     1861 aggtggagat ggtcgatggc cggttcacca agatcgaggg ctccgatcgc gagttgcccg
     1921 ccgacctggt gttcttggcg atgggcttcc tcggacccga gcatggcggc tggctcggcc
     1981 aactcggggt cggctacgac gagcgcggca acgtcgcccg cgacgccaac ttcatgtcga
     2041 gcgtgccggg cgtgttcgtg gccggcgaca tgggccgggg ccaaagcctg atcgtgtggg
     2101 cgatcgccga gggacggtcg tgcgcggccg gggtcgatcg ctacctgatg ggcgagaccg
     2161 acctccctgc cccgatcgcg ccgaccgccc gcccactgca gtgagcgggt gccagacgaa
     2221 tgggtgccag aagaaggggt gccaagagaa ggggtgccag aagaaggtga cggagcgcga
     2281 gcgcaggtgc cgcttacact tacgggcatg acttttcgac tcccggcagt gctggtgggc
     2341 gccgctgccc tcgtcggcct gaccgcgtgc ggcagcgaag ccggcaccgg tgcctcctcc
     2401 cagagcaccc gtgtcgagat cagccagcct gtgtacgtga ccgccccgcc cgtgaccact
     2461 gccgcgacga tcgccggcca ggaccagacg gtcggtggca tcgtcaacga agaacagcag
     2521 tacaccgtcg tgttcggcga ctacctgatc ggcatcgcca agaagcactg catctcgctc
     2581 gacaccttgg tcgcctacaa caattggcca gaaggcaaca agagggctgg caacccgaat
     2641 gacgtcgtgc gcatcccctc cggctcgtgc gcacccggca cggacctgag tgctgccgct
     2701 accggcagcg gccagacggg gcagacgggc ggggcaacgg cgccgacgcc gtcgagcgcc
     2761 gccccgacca ccgagtacac c
//
LOCUS       DAORNF010000447         2775 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_27502_length_2775_cov_1.801103, whole genome shotgun sequence.
ACCESSION   DAORNF010000447 DAORNF010000000
VERSION     DAORNF010000447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2775
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27502_length_2775_cov_1.801103"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>359
                     /locus_tag="PLV68_15635"
     CDS             <1..>359
                     /locus_tag="PLV68_15635"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02734.1"
                     /translation="GTGRDVTVTDVLPAGLTLVSFGGTGWVCDPAATACLLSGELAVG
                     DSAALTLVARVSGAAAAPRAVNRVSVANSTPDPVPSNDEDEDTVDVYWIDLAITKTLV
                     GTLMPEVTATYRLTITN"
     assembly_gap    360..369
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <370..807
                     /locus_tag="PLV68_15640"
     CDS             <370..807
                     /locus_tag="PLV68_15640"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02735.1"
                     /translation="CAPDGEIAHVQTDVVVTDTMPDGLTPTGASGAGFSCSITGQVVR
                     CISTATMAAGTSAVVDVTAMVARSAAGTSITNTASVASLVTADVVPSNNADSASAIVP
                     LVPDLPVSGANPALLITLAALLAGAGAVLLVVTRRRRKQIVSA"
     gene            821..893
                     /locus_tag="PLV68_15645"
     tRNA            821..893
                     /locus_tag="PLV68_15645"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:854..856,aa:Thr,seq:tgt)
     gene            1104..2579
                     /locus_tag="PLV68_15650"
     CDS             1104..2579
                     /locus_tag="PLV68_15650"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HRE02736.1"
                     /translation="MDNDIVFASDLAASTLANRVTRGELRRIVRGIYTTDLTHSLEEI
                     VNRNWAVIVGRSFPDAVITDRSAITGGKVGGYLYLAHDARERELALPGLTVIARRGAG
                     PLPGDVPMPGGLVLASTARALVDNTADSRSRGGRPRRTLDSAELDDWIDRRCRLYGEG
                     RLAADRQQAEALADSLSASANRISDLNRRIGMALGSRDAASGSRSLAARAAGHPYDPD
                     RIERFDTLVAALRSTPPQSRRGLDPGSSDFLVQSFYEAYFSNYIEGTTFTVDEARSIV
                     YEARPIPNRAADSHDVLGTYRIVSDPEEMRYRADSADEFIDLLRSRHALPMGGRPEIA
                     GEFKTKPNQAGQTLFVAPELVAGTLAEGWRRLAQLDTAWERSVYAMFLVSEVHPFPDG
                     NGRVARVMMNAELVAGNQSKIIVPTGYRSEYLGALRRISRDDDPTIYIKALRYLHDYT
                     SQVDWSSDGNANADLMRTNAFAEEDDAPRLTLPRTVTVDRL"
BASE COUNT          464 a          966 c          893 g          442 t
ORIGIN      
        1 ggcacgggac gcgacgtcac ggtgaccgac gtgttgccgg ccgggctgac gctggtgtcg
       61 ttcggcggca ccggctgggt gtgcgacccc gccgcgacgg cgtgcctgct gtcgggcgaa
      121 ctcgcggtcg gcgactcggc cgcgttgacc ctggtcgcca gggtgagcgg ggcagcggct
      181 gcgccacgcg cggtcaaccg tgtgtcggtc gccaactcga cgcccgaccc ggtgccctcc
      241 aacgacgagg acgaggacac ggtcgacgtg tactggatcg acctcgcgat caccaagacg
      301 ctcgtcggca cgctgatgcc ggaggtcacg gcgacctatc gcctgacgat caccaactan
      361 nnnnnnnnnt gcgctcccga cggcgagatc gcccacgtgc agaccgacgt ggtggtcacc
      421 gacacgatgc ctgacgggct cacacccacg ggtgcgagcg gtgccggatt cagctgttcg
      481 atcaccggcc aggtggttcg atgcatcagt accgccacga tggctgcggg gacgtcggcc
      541 gtggtcgacg tgaccgcgat ggtcgcccgc tcggctgctg gtacgtcgat caccaacacc
      601 gcctcggtgg ccagcttggt gacggcggac gtggtgccgt cgaacaacgc cgacagcgcg
      661 agcgcgatcg tgccgctggt gccggacctg cccgtgtcgg gcgccaaccc ggccctgctg
      721 atcacgctcg ccgcgctgct cgcgggtgcc ggagcggtgt tgctggtcgt cacccgccgt
      781 cgccgcaaac agatcgtgtc ggcctagcct gacccgatcc gccgagttag ctcagttggc
      841 agagcacttg acttgtaatc atgtggtcgc gggttcgatt cccgcactcg gctcgaatct
      901 gattgcctgt actgtccatc agagattgat gcgatcagcg cggtggtcgg tcctgtcgct
      961 gcacagtgtg aactttgcct cgccgggcgg ggcaggcgtg tcgcatgcgg tgtacatgac
     1021 gtctcgccgc gacgcgtatc tacgggtttc ttccagcggg tgggtctaaa ctacgggttt
     1081 cttccagcga gaggagggaa tccgtggata acgacatcgt gttcgccagt gatctggccg
     1141 cgagcactct cgccaaccgc gtcactcgcg gcgaactgcg gcgcatcgta cgcggcatct
     1201 acactaccga tctcacgcat tcactcgaag agatcgtcaa ccgaaactgg gccgtcatcg
     1261 tcggccgctc gttcccggac gcggtcatca ccgaccggtc agcgatcacc ggcggcaagg
     1321 tcggcgggta cttgtacctc gcgcacgatg cacgcgagcg tgaactggcc ctgcccgggc
     1381 tcacggtcat cgcccgccgc ggcgccggcc cgttgcccgg agacgtcccg atgccgggtg
     1441 ggcttgtgct ggcatccaca gcgcgagcac tcgtcgacaa caccgccgac tcccgatccc
     1501 gcgggggccg cccccgacgc accctggact ccgcggaact cgacgactgg atcgaccggc
     1561 gctgccgcct ctacggagag ggccgccttg ccgcagaccg ccagcaagcc gaagctctcg
     1621 ccgacagcct gtcagccagt gccaaccgca tcagtgatct caaccgacgc atcggcatgg
     1681 ctctcggcag ccgcgacgcc gccagcggca gccgttcgct ggcagcacgc gccgccgggc
     1741 acccctacga tcccgaccgc atcgagcgat tcgacacact cgtcgcggca ctacggtcga
     1801 ccccaccgca aagccgccgc ggtctcgatc caggatcgtc agactttcta gtccagtcgt
     1861 tctacgaggc gtacttctcc aactacatcg aaggcaccac gttcacggtc gacgaggcgc
     1921 gcagcatcgt ctacgaagcc cggccgatcc cgaaccgggc agccgacagc cacgacgtgc
     1981 tcggcacgta ccggatcgtg tcggaccccg aagagatgcg ctaccgggcc gacagcgctg
     2041 acgagttcat cgacctgctc cggtcgcgac acgcgctgcc gatgggcggc cgccccgaga
     2101 tcgccggtga gttcaagacg aaaccgaacc aggctggcca aacgctgttc gtcgctcccg
     2161 agctcgttgc cgggactctc gcggagggat ggcggcgact tgctcaactc gacacagcat
     2221 gggagcgatc ggtctatgcg atgttcctcg tctccgaagt ccacccgttc cccgatggca
     2281 acggccgcgt cgcccgggtc atgatgaatg ccgaactcgt cgccggcaac cagtccaaga
     2341 tcatcgtccc gaccggatac cgaagcgaat acctcggagc ccttcgccgg atcagccggg
     2401 acgacgaccc gactatctat atcaaggcac tgcgctacct ccacgactac acgtcgcagg
     2461 tcgactggtc cagcgatggc aacgccaacg ccgacctcat gcgaaccaac gccttcgccg
     2521 aggaggacga tgctccccgg ctgaccctgc cgcgcaccgt gacggtcgac cgactctgag
     2581 acgcgccggc acctgatcgg tcgccgagct aggcggggag gacggcggtg ccgacgcggc
     2641 ggcggtggga tgagggggaa ccccaggcgg ggatcagcgc ccaggccttc ttcatgtaga
     2701 ggtgcaggtc gcactcgacc gtgtagccga tcgcaccgtg cacctgcagc gacgtggcgg
     2761 ccgcggcctt ggcgg
//
LOCUS       DAORNF010000448         2770 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_27569_length_2770_cov_1.471087, whole genome shotgun sequence.
ACCESSION   DAORNF010000448 DAORNF010000000
VERSION     DAORNF010000448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2770
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27569_length_2770_cov_1.471087"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>233)
                     /locus_tag="PLV68_15655"
     CDS             complement(<1..>233)
                     /locus_tag="PLV68_15655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02737.1"
                     /translation="VGFDGKVAIITGAGGGLGRQHALLLAKRGALVVVNDLGGAIDGT
                     GSDKGAAERMVDEIKAADPAGIIFSGGPASVHAP"
     assembly_gap    234..435
                     /estimated_length=202
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<436..>1494)
                     /locus_tag="PLV68_15660"
     CDS             complement(<436..>1494)
                     /locus_tag="PLV68_15660"
                     /inference="COORDINATES: protein motif:HMM:NF015706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02738.1"
                     /translation="AAVAVEIAADPAGHPVALVFKTVVDQFLGNLSMVKVVSGTIANE
                     ARLVNSATGADERVHGLYRVRGRDHQPVQALHAGEIGAIAKLTDSPTGTTLSGPGQPV
                     HVAWLSPPAPNYALALEPLTQADDDKLTVALTRAVAEDPSLAIERSDETHQTVLAGRG
                     DMHLTVTLERLSRKFGVNVSTADVAIAYRETISAGAGAQGKVKKQSGGHGQFAVCDLR
                     VTPTQRGTGSSFVDSVVGGAIPRQYLPAVERGAFEAMEAAGPLGFPVVDVRVECHDGK
                     VHSVDSSEMAFRAAAGAGVREALSKAGPVLLEPVSHVTITVPETQQGDVLGDLSARRG
                     RVVGSAALDDGLHEISALV"
     assembly_gap    1495..1618
                     /estimated_length=124
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1619..2392)
                     /locus_tag="PLV68_15665"
     CDS             complement(<1619..2392)
                     /locus_tag="PLV68_15665"
                     /inference="COORDINATES: protein motif:HMM:NF012239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRE02739.1"
                     /translation="MPTPRAPGANTAIRNVAVVGHSSSGKTTLVEALLHRSGVLTRAG
                     SVDDGTTVTDHEPEEHKRRISLSLGIAPLDWKADDGNTYSITLLDTPGYPDFSGEIDA
                     ALAVADLAVVVVSAVDGVQVGTERAWRRCVAAGVPRLVFVTKEDKEHADFGRVLDQLR
                     ASFGGSFVPLELPLGEAGELHGVADVLSDVAFEYEADGTHHTETLPADVADAEHALHD
                     AVVEEIVAGDDVQLERYLSGETMSPEELEHVLAAEMLAGT"
BASE COUNT          378 a          844 c          838 g          384 t
ORIGIN      
        1 ggggcgtgaa cgctggcggg tccgccgctg aagatgatgc ccgcggggtc cgcggccttg
       61 atctcgtcga ccatccgctc ggcggcgccc ttgtcgctgc cggtgccgtc gatcgccccg
      121 cccaggtcgt tgacgaccac cagcgcgccg cgcttggcca gcagcaacgc gtgttgccgc
      181 cccaggccgc cacccgctcc cgtgatgatc gctaccttgc cgtcgaaacc cacnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnncacca acgcggagat ctcgtgcaag ccgtcgtcga gtgcggccga
      481 cccgacgacg cgtccgcggc gcgccgacag gtcaccgagg acatcgccct gttgcgtctc
      541 cggcaccgtg atggtcacgt gcgacaccgg ttcgagcagc actgggccgg ccttgctcaa
      601 cgcttcacgc actcccgcgc cagcagcggc ccggaacgcc atctccgacg agtcgaccga
      661 gtgcaccttg ccgtcgtggc actcgacgcg cacgtcgacc accggaaacc cgagcggacc
      721 cgcagcctcc atcgcctcga acgcaccccg ctcgacggcg ggcaggtact ggcgaggaat
      781 tgccccgccg acgacggagt cgacgaagct cgatccggtg ccgcgctggg tgggggtgac
      841 gcgcaggtcg cacaccgcga actggccgtg accgccgctc tgcttcttga ccttgccctg
      901 ggccccggca cctgcagaga tcgtctcgcg ataggcgatc gcgacgtcgg cggtcgacac
      961 gttgacgccg aacttgcgcg acaggcgttc gagcgtgacg gtcaggtgca tgtcgccgcg
     1021 gccggccaag accgtctgat gggtctcgtc ggaccgctcg atcgcgaggg acgggtcctc
     1081 ggccaccgcg cgcgtcagcg cgacggtcag cttgtcgtcg tcggcctggg tcagcggctc
     1141 gagtgcgagg gcgtagttgg gtgcaggggg cgagagccag gcgacgtgca ccggctggcc
     1201 cgggcccgac agcgtggtgc cggtcgggct gtcggtcagc ttggcgatgg cgccgatctc
     1261 gccggcgtgc agtgcttgca ccggctggtg gtcacgaccg cgcacccggt acaggccgtg
     1321 gacgcgctcg tcggccccgg tggccgagtt gaccagccgg gcctcgttgg cgatggtgcc
     1381 ggacacgacc ttgaccatcg acaggttgcc gaggaactgg tcgaccacgg tcttgaacac
     1441 cagcgcgacc gggtgacctg cgggatcggc tgcgatctcc accgccacgg cagcnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngg
     1621 tgcccgccag catctcggcc gcaagcacgt gctcgagctc ttccggcgac atcgtctcac
     1681 ccgacaggta gcgctcgagc tgtacgtcgt caccagcgac gatctcttcc accaccgcgt
     1741 cgtgcagggc gtgctcggca tcggcgacgt cggctggcag ggtctcggtg tgatgggtgc
     1801 cgtcggcctc gtattcaaag gcgacgtcgg acagcacgtc ggccacgccg tgcagctccc
     1861 ctgcctcgcc gaggggcaac tcgagtggaa cgaagctgcc gccgaagctt gcccggagct
     1921 gatccagcac cctcccaaag tcggcgtgtt ccttgtcttc cttggtgacg aacaccagcc
     1981 gcggcacccc ggcggcgacg cagcgtcgcc acgctcgttc ggtaccgacc tgcacgccgt
     2041 cgactgcgct gaccaccacg acggccagat cggcgacggc aagggccgcg tcgatctcgc
     2101 ctgagaagtc gggatatccg ggtgtatcga gcaacgtgat cgagtaggtg ttgccatcgt
     2161 cagctttcca gtcgagcggg gcgataccga gcgacaacga gatgcgccgc ttgtgctctt
     2221 cgggctcgtg atcggtgacc gtggtgccgt cgtcgaccga accggcgcgg gtcaggaccc
     2281 cggagcggtg caacagtgcc tcgaccaggg tggtcttgcc gctgctgctg tggccgacga
     2341 cggccacgtt gcggatcgcg gtattcgctc caggcgcgcg aggggtcggc atgagcatcc
     2401 tccgaactgc gaggcaccag cccgacgccg gcgctgttgg tcgcgaccgt acgcggtccg
     2461 cggcctgcgg cgctacgggc aattgtcccg gtcagaggcg gcgagaggtc ctggccatca
     2521 gcgcgaggcg aagccgtccg ccaatatctg gcgacattcc gtagggcgag gttgcgcccg
     2581 tgtcgacacc tcgctacagt ttcgatccgc tcagcacagc ctccgggctc gggcagagcg
     2641 agacaccaca gcgctggcac caacgccggt caggtcgtca acacgacgat cgatcgagcg
     2701 cgtaccagcg aagcgggttc tttgaaaacg gaagagaaga ctgaatgaga gtgcgggtga
     2761 cgcgccgttt
//
LOCUS       DAORNF010000449         2769 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_27579_length_2769_cov_1.704127, whole genome shotgun sequence.
ACCESSION   DAORNF010000449 DAORNF010000000
VERSION     DAORNF010000449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2769
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27579_length_2769_cov_1.704127"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>280)
                     /locus_tag="PLV68_15670"
     CDS             complement(<1..>280)
                     /locus_tag="PLV68_15670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02740.1"
                     /translation="GEDAARTWLEDMIANDVVTDIASNGDVLKAVNDGEIEMGLINHY
                     YWAGLAAELGVDNMKAQLIFPKGDDPGGLFNATAVGITNTGADNPAALA"
     assembly_gap    281..337
                     /estimated_length=57
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<338..965)
                     /locus_tag="PLV68_15675"
     CDS             complement(<338..965)
                     /locus_tag="PLV68_15675"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRE02741.1"
                     /translation="MIKRSRIAVLAAAALALTAVSCGSDEKAATTTESTTTSAPAVTE
                     PSTVETDAPASSTTAAETTTTAASTTAAPAEPGTLVLYSGRNEGLVAPLIEQFEKETG
                     IKVEVRYGSSSEMGAQLLEEGDKTPADVFYSQEVGALGMLSIAGLLSSLPDDVVALAD
                     ERFQPADGNHWVGVTGRSRVIVYNPDLVPEPPTGVLELTDAKWAGKVAI"
     gene            complement(1101..>1622)
                     /locus_tag="PLV68_15680"
     CDS             complement(1101..>1622)
                     /locus_tag="PLV68_15680"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRE02742.1"
                     /translation="APPMRLSEALAMPAGRPTLTIATTWDDFVSFARTGRPPGHCTAL
                     GWVLDPHRRAILLVRHRVLGWTCPGGHVEPGEALHEAASRELREETGLRLAPVSEQPL
                     TLTLAHGCHAPGSADHDHWGFGFRFHAASDAAVLATEPGQPAQWFAVDALPSRRPADI
                     DAVVAHLRATDWA"
     assembly_gap    1623..1652
                     /estimated_length=30
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1653..2199)
                     /gene="folE"
                     /locus_tag="PLV68_15685"
     CDS             complement(<1653..2199)
                     /gene="folE"
                     /locus_tag="PLV68_15685"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HRE02743.1"
                     /translation="MTDPHAHSFDHARAEAAVRELLLAIGEDPDRDGLLATPGRVARM
                     FAEVTTGLSEDPAKHLKTVFEAGHDEMVLVRDIPFASMCEHHLLPFIGNAHVGYIPGP
                     SGHVTGRSKLARLVDGYARRLQVQERLTTQIAAAIEETLQSTGVIVVLEAEHLCMSIR
                     GVAKPGALTVTSAVRGQFRDDP"
     gene            complement(2251..>2769)
                     /locus_tag="PLV68_15690"
     CDS             complement(2251..>2769)
                     /locus_tag="PLV68_15690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRE02744.1"
                     /translation="LGPTRMTFTKPTIAAVEGYAVAGGLELAMMCDLRVAARDAVFGV
                     FCRRWGVPLVDGGSIRLSRMLGHSHALDLILTGRPVSGDEARLMGLANRLTEPGDALA
                     AALVMAHQLAALPQRCMRNDRTSSYEQWDLSWRDALARETELGLEVLRSREAQQGAAR
                     FAAGEGRGGAPA"
BASE COUNT          418 a          909 c          923 g          432 t
ORIGIN      
        1 aggccagcgc ggccgggttg tcggcgccgg tgttggtgat accgacggcg gtcgcgttga
       61 acagtccacc cgggtcgtcg cccttgggga agatcagctg cgccttcatg ttgtcgacgc
      121 ccagctccgc ggccaggccg gcccagtagt agtggttgat gagacccatc tcgatctcgc
      181 cgtcgttcac agccttgagc acatcaccgt tgctggcgat gtcggtcacg acatcgttgg
      241 cgatcatgtc ttccagccat gtgcgagcag cgtcttcgcc nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncga tcgcgacctt gccggcccac
      361 ttggcatcgg tcagctccag caccccggtc ggcggctcgg ggaccaggtc ggggttgtag
      421 acgatgacgc gcgaacggcc ggtgacaccc acccagtgat tgccgtcggc gggctggaag
      481 cgctcgtcag ccagagcgac cacgtcgtcg ggcaacgagg acagcagacc agcgatgctg
      541 agcatcccga gcgcgccgac ctcttgtgag tagaacacgt cggccggggt cttgtcaccc
      601 tcttccagca gttgggcacc catctcggac gagctgccgt agcggacctc gaccttgatg
      661 ccggtctcct tctcgaactg ctcgatcaac ggggcgacga ggccctcgtt gcggccgctg
      721 tacagcacga gcgtgccagg ctcggccgga gccgcggtgg tggaagcagc ggtcgtggtc
      781 gtctcggcgg ccgtggtgga cgaagccggc gcatcggtct cgaccgtgga cggctccgtg
      841 accgcagggg ctgacgtcgt cgtggactcg gtcgtggtcg cggccttctc gtcgctcccg
      901 caactgaccg cggtgagtgc gagtgctgca gcggcaagca cagcaattcg ggaacgtttg
      961 atcattgggg gtttctcctc gatgcatcga tgggattggc atcggcatgt taggcatgcc
     1021 gatgccgtgg tgcaaagaag ctggctcacc cgtccttcac gcgcccatcc atcaggctcg
     1081 ctccggcatc acgtcattcg tcaggcccag tcggtggcac gcaggtgggc cacgacggca
     1141 tcgatgtcgg ccgggcgtcg cgacggcagc gcgtctacgg cgaaccactg cgccggttga
     1201 cccggttcgg tcgcaagcac cgccgcgtcg gacgcggcgt gaaaacggaa gccgaatccc
     1261 caatggtcgt gatcggccga gcccggcgcg tggcatccgt gcgcaagggt cagggtcagc
     1321 ggttgctcgc taaccggtgc gagcctcaac ccggtctctt cgcgcaactc gcggctggcc
     1381 gcctcgtgca gcgcttcgcc tggttcgacg tgcccaccgg gacaggtcca tccgaggacc
     1441 cggtgacgga cgagcaggat cgcccggcga tgcgggtcga gcacccagcc aagcgcggtg
     1501 cagtggccgg gcgggcgacc cgtgcgcgcg aacgagacga agtcatccca cgtcgtggcg
     1561 atcgtcaggg tcgggcggcc cgccggcatg gcgagcgcct cggacagccg catcggaggc
     1621 gcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngcgggtcg tcccggaact ggccgcgcac
     1681 cgccgaggtc accgtcagcg cacccggctt ggcgacgccc cggatcgaca tgcacaggtg
     1741 ctcggcttcg agcacgacga tcacacccgt cgactgcaac gtctcctcga tggctgccgc
     1801 gatctgtgtc gtcagtcgct cctgcacctg gagccgccgg gcgtagccgt cgaccaggcg
     1861 ggccagcttc gaccgccccg tgacgtgtcc gctcggcccc gggatgtacc cgacgtgggc
     1921 gttgccgatg aacggcagca ggtggtgctc gcacatcgac gcgaatggga tgtcgcgcac
     1981 cagcaccatc tcgtcgtggc cagcttcgaa cacggtcttg agatgcttgg cgggatcttc
     2041 cgacagtccg gtggtcacct ccgcgaacat tcgtgcgacg cggccgggag tcgcgagcag
     2101 tccatcgcga tcgggatctt caccgatcgc gagcagcagt tcgcgcacgg ccgcctcggc
     2161 gcgggcgtgg tcgaacgagt gagcgtgggg gtcggtcatc catccagcat gccggagcac
     2221 gcatcgttcc acgccccggc cgcagcgctt ctacgccggg gcaccaccgc ggccttcacc
     2281 ggctgcaaac cgggcggcac cctgttgtgc ctcacgtgag cgcaacacct cgaggccgag
     2341 ctcggtctca cgggcgagtg catcgcgcca cgacaggtcc cactgctcgt aactcgatgt
     2401 gcggtcgttg cgcatgcacc gttgtggcag agcagccagt tgatgggcca tcacgagcgc
     2461 cgcggcgagc gcgtcgcccg gctcggtgag gcggttggcg aggcccatca ggcgggcttc
     2521 gtcaccgctg accggacggc ccgtgaggat caggtcgagc gcgtggctgt gaccgagcat
     2581 gcgcgagagg cggatcgatc cgccgtcgac gagcggcacg ccccaacggc gacagaacac
     2641 accgaacacc gcgtcgcggg cagcgacgcg caggtcgcac atcatcgcca actccagacc
     2701 accggccacg gcgtagccct cgaccgcggc gatcgtgggc ttggtgaacg tcatccgcgt
     2761 cggcccgag
//
LOCUS       DAORNF010000450         2768 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_27594_length_2768_cov_1.403981, whole genome shotgun sequence.
ACCESSION   DAORNF010000450 DAORNF010000000
VERSION     DAORNF010000450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2768
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27594_length_2768_cov_1.403981"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>223
                     /locus_tag="PLV68_15695"
     CDS             <1..>223
                     /locus_tag="PLV68_15695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02745.1"
                     /translation="FGVIWDTATDGHPRPASVRVRRDDALRGVPTWVVSGAVPIDASH
                     ALGADAAAVTEQRLTGYGWTVEPVARASGW"
     assembly_gap    224..319
                     /estimated_length=96
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    655..690
                     /estimated_length=36
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<691..1095)
                     /locus_tag="PLV68_15700"
     CDS             complement(<691..1095)
                     /locus_tag="PLV68_15700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02746.1"
                     /translation="MTPWLGPVNYDRTTHFSERATGGQHRSAYYDPFTDTYFRYQHGR
                     EICFGGYVLCLYFKADSFTSAFRLVGWDYSDRYARLIDAEGIGMFNRPADFPSGYFTA
                     LLTGSGRCYPPGYNADILRSGGYVDMVNVGPNW"
     gene            complement(1092..>1991)
                     /locus_tag="PLV68_15705"
     CDS             complement(1092..>1991)
                     /locus_tag="PLV68_15705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02747.1"
                     /translation="VIVDVLGWFPTDGPFTGLTPARLIDTRPGGPTTDGLGSGGGALR
                     ANSTATYQISNRGGIPASGVSAVAINLTAASTTASGYLTVYPFGSAKPTASNLNFTRG
                     QIVPNMAIVPLGPDGRITVYNFSGTTDVVIDVLGYFTTGTAFTGLTPARLLDTRIGFP
                     TTDNLFDGTREVIADTVNNVRIGARPGVPADAGSVVLNVTVTGSTGPGFLTIYPTGAK
                     RPTASNLNFTAGATVANMVTVPLGNGGQIAIYAGVSSVDVIVDVLGWYPNQPTPPPTT
                     ALRSPWATLVGTISQLPMSISRL"
     assembly_gap    1992..2108
                     /estimated_length=117
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2145..>2388)
                     /locus_tag="PLV68_15710"
     CDS             complement(2145..>2388)
                     /locus_tag="PLV68_15710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02748.1"
                     /translation="SMTAVAAATLIATQTVAPTASVAAAVAPTFNGMTPVRLLDTRDS
                     GPLTPLSSRELVVTTAGVPAEAGAVARDKGRGPLRT"
     assembly_gap    2389..2516
                     /estimated_length=128
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          391 a          715 c          889 g          396 t
ORIGIN      
        1 gttcggcgtg atctgggata ccgccaccga cggtcaccct cgcccggcct cggtgcgagt
       61 gcgtcgcgac gacgcgctgc ggggcgtgcc cacctgggtg gtgtccggtg cggtcccgat
      121 cgacgcgtcc cacgccctcg gcgccgacgc ggccgccgtg accgagcagc gcctgaccgg
      181 ctacggctgg accgtcgaac cagtcgcacg cgccagcggc tggnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnna ctaccacctg agcccgccat gggcgacgtg aaacagccga
      361 cacgttccac gaacagactc ctgctgccct gcccggccca caccgactaa ccgccgcccc
      421 gggccaatcc tggcctcggt tcacctgagc cgggtagctg gattgggtat ccgaacctga
      481 aggctgttcg tctgcacctc aagttgccgt acatcgccaa ctacgaggcc gatgactcga
      541 cgctgctgct ggtgcgtgtg cttcacgaga gccgttcact cgaacgtgcc gtcggcgagg
      601 cgtagcaccg agaacgccga ggggggcggg tgtcagccgc acccaggcca gatcnnnnnn
      661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ccagttgggg ccgacgttca ccatgtcgac
      721 gtatcccccg gatcgaagaa tgtcggcgtt gtagccgggt ggatagcagc ggcccgagcc
      781 agtcaacagg gccgtgaagt agccgctggg gaagtcggcg ggccgattga acataccgat
      841 tccctcggca tcgatgagac gggcgtagcg gtcggagtag tcccaaccaa cgagccggaa
      901 cgctgacgtg aagctatcgg ccttgaagta gaggcacagg acgtacccgc caaagcagat
      961 ctcgcggccg tgctgatagc ggaaataggt gtcggtaaac gggtcgtagt aggcgctgcg
     1021 atgctgccca ccggtagctc gctcgctgaa gtgtgtggtc cggtcgtagt tgacgggtcc
     1081 caaccatgga gtcacagcct cgatatagac atcgggagtt gcgaaatagt ccccaccaaa
     1141 gttgcccagg gagagcggag agccgtagtt ggtggcgggg tcggttggtt gggataccaa
     1201 cccaacacgt cgacgatgac gtccacgctg gacactccgg cgtagatcgc gatctgcccg
     1261 ccgttgccga gggggacggt gaccatgttg gccactgtcg ccccggcggt gaagttgagg
     1321 ttcgacgcgg tgggccgctt cgccccggtg ggatagatgg tcaggaagcc gggtccggtc
     1381 gagcccgtga cggtgacgtt gaggacgacc gacccggcgt cggcgggcac tcctggccgg
     1441 gcaccgatgc gcacgttgtt cacggtgtcc gcgatgacct cgcgggtgcc gtcgaagagg
     1501 ttgtcggtgg tcgggaagcc gatgcgggtg tcgagcagac gggcgggggt cagcccggtg
     1561 aatgctgtgc cggtggtgaa gtagccgagg acgtcgatca cgacgtcggt ggtcccactg
     1621 aagttgtaga cggtgatccg cccgtcgggt ccgaggggga cgatggccat gttgggcacg
     1681 atctggccgc gggtgaagtt gaggttggaa gccgtcggct tggcggaccc gaacgggtac
     1741 acggtgaggt agccagacgc cgtggtcgag gccgcggtga ggttgatcgc aacggcagac
     1801 accccagatg cggggatgcc gccacgattg ctgatctggt acgtggccgt actgttggcg
     1861 cggagtgctc cgcccccgga gccgagcccg tcggtggtgg gtccgcctgg gcgagtgtcg
     1921 atcaggcggg ccggggtgag gccggtgaac gggccgtcgg tcgggaacca gccgaggacg
     1981 tcgacgatga cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnca acaaccgcaa ccgcagcagg tcacgcgcca gcattcaggt tcgcaagggt
     2161 cctcgtccct tgtcgcgcgc gaccgcgcca gcctcggcgg gcacacctgc ggtggtgacc
     2221 acgagttcgc gcgagctgag cggcgtgagc ggcccgctat ctctggtgtc gagcagccga
     2281 acgggcgtca tcccgttgaa cgtgggcgcg acggctgccg caaccgatgc ggtgggggcg
     2341 acggtctgcg tcgcgatcaa cgttgctgcg gcgaccgccg tcatcgacnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnggtg
     2521 acggtgttca gttcgccccg tctccggggg tcaaggcgac acgggtgcgg gtgggtgcaa
     2581 tggggcggca tctactgtgg gggcgtgggt gtgccgatgc gtgtggccga gttgtggcgg
     2641 tatccggtca agtcgatgca gggcgagttg ctcgacgcgg cggagatcgg tgaggcgggc
     2701 atcgtcgggg atcggcagtg ggcgttgcgt gacgtgacga cggggcacgt cctcaccgct
     2761 cggcgtgt
//
LOCUS       DAORNF010000451         2765 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_27638_length_2765_cov_1.573063, whole genome shotgun sequence.
ACCESSION   DAORNF010000451 DAORNF010000000
VERSION     DAORNF010000451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2765
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27638_length_2765_cov_1.573063"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    266..296
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            317..772
                     /locus_tag="PLV68_15715"
                     /pseudo
     CDS             317..772
                     /locus_tag="PLV68_15715"
                     /inference="COORDINATES: protein motif:HMM:NF014757.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
     gene            882..1124
                     /locus_tag="PLV68_15720"
     CDS             882..1124
                     /locus_tag="PLV68_15720"
                     /inference="COORDINATES: protein motif:HMM:NF012928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HRE02749.1"
                     /translation="MFESGFASAEDIDTGMIEGCNHPMGPLHLADLIGLDTTLAVAES
                     LYAEFKEPLYAPPPLLSRMVEASLLGRKTERGFFAY"
     assembly_gap    1196..1262
                     /estimated_length=67
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1263..1617)
                     /locus_tag="PLV68_15725"
     CDS             complement(<1263..1617)
                     /locus_tag="PLV68_15725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3052 domain-containing protein"
                     /protein_id="HRE02750.1"
                     /translation="MTAAPDVPLIDKLGIAEGTRLAVTGAPTHFRASLGELPLAVEWA
                     NRVRPPLDLVVSFHTSRAAMVQQWPALTGAVVPTGAIWVAWPKKTSGVTTDITDAVIR
                     ETLLSGTWVDNKVCSI"
     gene            complement(1685..>2765)
                     /locus_tag="PLV68_15730"
     CDS             complement(1685..>2765)
                     /locus_tag="PLV68_15730"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HRE02751.1"
                     /translation="RRTARTAAPVLIGVALLAGASVFASSLKAQLRQTIGELFIGDYV
                     INSTNGGPVSFGPSVVDDLNAIPEVGAATGLGIASVADADGKPMLMRAVNGPFAEGLL
                     NLQFVAGSFADLTSDGMLISESEARRKNLAIGSSFDVRITDVTKRVTVQGIFVASDFA
                     GARVVDNSFFDATAESNSFGFVVLTNAPGVSDARFRAAVEQVLTDYGIGELQDRDEFI
                     DGRGDIIDQSLSFIYGLLGLSVVIAVFGIVLTMLLAVYERRREIGLLRAVGMTRSQVR
                     TVVRWESVLTSLYGAAVGVGLGLVLGYVVILALRDEGLTTFAVPVQAIGVIVVAAFVT
                     GVLAAVIPAWRATRLNVLESIAIGS"
BASE COUNT          444 a          883 c          931 g          409 t
ORIGIN      
        1 accgtggttt cgctgggtac gacagggatc accgagtcga ggaatgcgat cacgacgatc
       61 gccacgtaga accacggtga gctggagatc tcctgcagtc ggtcgagcac cgtgtcgagc
      121 aggccgaaca tggccttacg ctaaccgtcg ccgctgccat acctcgctcg cggagatgtc
      181 gcacaccagc gctggacggt cggcccgcgg gggtaggcct ggccggacat tcggtcgtgg
      241 ggttggtggg ctcggtaagg tcgtgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngtgt
      301 ggttgggtgc ggtttgatgg gttcgggcat cgccgaggtc tgtgcccgcg ccggtctcga
      361 cgtgacggtg gtcgaagggt cgcaggcggc cgccgacgcc ggcatccagc gcatcaccaa
      421 gtcgctcgac cgggctgtgc gggcgggcaa gctcgacgcg ggcgacaaag atcgcttcct
      481 cgacggcatc cgcgtcagca ccgatctcgg acggctcgaa ccgtgccagg tcgtgatcga
      541 agcgatcgcc gaggatcagg agctcaagat ccgcctgttc caacagctcg acgaggtgat
      601 gcacggtgcc gacgccatcc tcgcctcgaa caccagctcg atcccgatca tgaagctcgg
      661 catcgccacc cgccggcccg atctggtggt cggcgtgcac ttcttcaacc cggtcccggt
      721 gttgaagctg gtcgagatcg tgccctcgct gctgacgtcg gacgagacgg tcgccgacat
      781 gtcggccttc gctaccgacg tgttgggcaa gcgcacgatc cgttgccagg accgggccgg
      841 gttcgtggtc aacgatcccg tacatcctct ccgcgatccg catgttcgag agcgggttcg
      901 cctcggccga agacatcgac accggcatga tcgaggggtg caaccacccg atgggaccgc
      961 tgcacctggc cgacctgatc ggcctcgaca ccaccctcgc cgtggccgag agcctctacg
     1021 ccgagttcaa ggaaccgctc tacgcgccgc cgccgctgct cagccgcatg gtcgaagcca
     1081 gcctcctcgg ccgcaagacc gaacgcggtt tcttcgccta ctgaccgacc gggtcgggcg
     1141 atgcccggtc agcggcggcg ggacttggag agcttggtgg gggcgctcgc agcggnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nncgatcgag cagaccttgt tgtcgaccca ggtgccagac agcagcgtct cgcggatcac
     1321 cgcgtcggtg atgtcggtcg tgactccgct ggtcttcttc ggccacgcca cccagatcgc
     1381 tcccgtcggt acgacggcac cggtcagggc gggccactgc tgcaccatcg cggcgcggct
     1441 ggtgtggaag ctgacgacga gatcgagcgg tgggcggacc cggttggccc attcgaccgc
     1501 caggggtagc tcgccgagcg acgcgcggaa gtgggtcggg gcgccggtga cggccagacg
     1561 agtgccctcg gcgatgccga gcttgtcgat cagcgggacg tcgggggcgg cggtcatggt
     1621 gccgggcagc gtaacgccgc agcgcccggg ccgtctggac cgatccgtgt cacgcgatgc
     1681 cgggtcagga accgatcgcg atcgattcga gcacgttgag gcgggttgcc cgccacgcgg
     1741 ggatcacggc ggcgagcaca cccgtgacga acgcggccac gacgatgacc ccgatggcct
     1801 gcaccgggac ggcgaacgtg gtcaggccct cgtcgcgcag ggccaggatc acgacgtagc
     1861 cgagcaccag gccgagcccg acgccgaccg ctgcgccgta gagcgaggtg agcacgctct
     1921 cccagcgcac gacggtgcgc acctgtgagc gggtcatgcc caccgcgcgg agcagaccga
     1981 tctcacggcg acgctcatag acggcgagca gcatcgtcag cacgatgccg aacacggcga
     2041 tcacgaccga cagcccgagc agtccgtaga tgaacgacag gctctggtcg atgatgtcgc
     2101 cgcggccgtc gatgaactcg tcccggtctt gcagttcgcc gatgccgtag tcggtgagca
     2161 cctgctcgac cgcggcgcgg aaccgcgcgt cggacacacc cggcgcgttg gtcagcacca
     2221 cgaagccgaa cgagttggac tcggcggtgg cgtcgaagaa cgagttgtcg accacgcgtg
     2281 ccccggcaaa gtcggacgcg acgaagatgc cctggaccgt gacccgcttg gttacgtcgg
     2341 tgatgcggac gtcgaagctc gaaccgatcg caaggttctt gcggcgcgct tcggactcgc
     2401 tgatcaacat tccgtcgctg gtcaggtcgg cgaaggatcc ggcgacgaac tgcaggttga
     2461 gcaacccctc ggcgaacggg ccgttgacgg cgcgcatcag catcggcttg ccgtcggcgt
     2521 cggcaaccga tgcgatgccc agcccggtcg ccgcgccgac ttcggggatc gcgttgaggt
     2581 cgtcgaccac cgatggcccg aagctgaccg gcccaccgtt ggtggagttg atcacgtagt
     2641 caccgatgaa cagctcgccg atcgtctggc gcagctgcgc cttgagcgac gaggcgaaca
     2701 ccgacgcgcc ggccagcagc gcgacgccga tcagcacggg ggccgcggtg cgggcggtgc
     2761 gccgc
//
LOCUS       DAORNF010000452         2753 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_27784_length_2753_cov_1.905856, whole genome shotgun sequence.
ACCESSION   DAORNF010000452 DAORNF010000000
VERSION     DAORNF010000452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27784_length_2753_cov_1.905856"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="PLV68_15735"
     CDS             complement(<1..323)
                     /locus_tag="PLV68_15735"
                     /inference="COORDINATES: protein motif:HMM:NF016144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HRE02752.1"
                     /translation="MAADPVWPERFARARERIAAALDAAALAIEHVGSTSVPGLSAKP
                     VIDIDLTVADSSDEGAWLPQLEAAGFALAVREPDWEQHRMLRGADPAVNLHVFSAGAR
                     EPQRHL"
     assembly_gap    377..419
                     /estimated_length=43
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <420..783
                     /locus_tag="PLV68_15740"
     CDS             <420..783
                     /locus_tag="PLV68_15740"
                     /inference="COORDINATES: protein motif:HMM:TIGR01552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HRE02753.1"
                     /translation="RSPPLAPARGCYRACEQHDRLVAASESLIFRYAEEMRTISATDA
                     SRRFSDLLDAIEAGESVVVTRGNRPIAEVRPAIRRTGRDLRAALAEMEPPDERFAADV
                     EGAVAGLIQEGDDPWAAD"
     gene            768..1172
                     /locus_tag="PLV68_15745"
     CDS             768..1172
                     /locus_tag="PLV68_15745"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HRE02754.1"
                     /translation="MGRRLILDTNVLIDYERGAFDRSVLDDDDLAIAALTVAEYRTGI
                     ELADSVERAAARSRALAVITEMIDVLDYTEATAVHHARLIANARQSGRRRAAHDMIIA
                     AHAAQTGRTLLSRDAAARFGDLPGIAAENPPV"
     gene            complement(1182..1257)
                     /locus_tag="PLV68_15750"
     tRNA            complement(1182..1257)
                     /locus_tag="PLV68_15750"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1222..1224),aa:His,seq:gtg)
     gene            1402..>1860
                     /locus_tag="PLV68_15755"
     CDS             1402..>1860
                     /locus_tag="PLV68_15755"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRE02755.1"
                     /translation="MSATDSSLTHSPQMRNLTERARTITRRELAIYAERTQASQRATE
                     RARQVLPLGVPSSFQAYDPHPIVVRQAQDSWMEDVDGNRYIDYDMGFGALFAGHCHPA
                     VRRAIEAQLDMGTLFVTPCEDNATVAELLGQRYGLPMWRFTNSGTEATMDA"
     assembly_gap    1861..1870
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1871..>2454
                     /locus_tag="PLV68_15760"
     CDS             <1871..>2454
                     /locus_tag="PLV68_15760"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRE02756.1"
                     /translation="RGVTGRQKLVKVEGGYHGHHDEVMISMKPPLDQAGPADAPNSVP
                     STAGITDAVLADTIVIPYNDPDALERALRGGDVACFIVEPVMENIGICRPVPGYLEAV
                     REITARYGTLLIFDDVKTGITAGWSGATGAFGVLPDLVTLAKSIGGGLPLGAFGGKRE
                     YMDQITQGRVLHLGTYNGNPLCMAAAVAVLDQVC"
     assembly_gap    2455..2464
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2465..>2753
                     /locus_tag="PLV68_15765"
     CDS             <2465..>2753
                     /locus_tag="PLV68_15765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02757.1"
                     /translation="LADICTPETQQAVNALNGKLVAACQNTIDAAGLPAHTVQFGAKG
                     CVTWSVEPIKNYRDYKATDFDLAFAQWIHGINRGVLLPPGLDEQWLISVLHT"
BASE COUNT          466 a          960 c          864 g          400 t
ORIGIN      
        1 aggtgccgct gtggttcgcg agcgccggcc gagaacacgt gcaggttcac cgccgggtcg
       61 gcgccgcgca gcatgcggtg ttgctcccag tcgggttcgc gaaccgccag cgcgaagccg
      121 gccgcttcca gttgtggcag ccatgcgccc tcgtcagacg agtcggcgac ggtgagatcg
      181 atgtcgatca ccggcttcgc ggacagcccc ggtaccgacg tcgaaccgac gtgttcgatc
      241 gcgagcgcgg cagcgtccaa cgcggcggcg atccgctccc gagcacgcgc aaaccgctcc
      301 ggccacacgg gatcagccgc caccacctcg acgtgactgg tctcgcaacg aggcgtcccg
      361 acgcgagccg cggcgannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc
      421 cgatcacccc ctctcgcccc ggcccgcgga tgctaccgag cctgcgaaca acacgaccgc
      481 ctggttgccg cgtccgagag tctgattttc agatacgctg aggaaatgcg gacgatcagc
      541 gcgaccgatg cctcccggcg cttttccgac ttgctcgacg cgatcgaggc gggcgagtcg
      601 gtcgtggtga ccagagggaa ccgaccgatc gccgaggtcc gtcccgcaat ccggcgaacc
      661 gggcgggacc tgcgcgccgc ccttgccgag atggaaccgc ccgacgagcg cttcgccgcg
      721 gatgtcgagg gtgcagtcgc cggtctcatc caagaaggcg acgacccgtg ggccgccgac
      781 tgatcctgga caccaacgtg ctcatcgact acgagcgagg agcgttcgat cggtccgtgc
      841 tcgatgacga cgacctggcc atcgccgcgc tcacggtggc ggaataccgc accggcatcg
      901 aactggccga ttcggtcgag cgcgccgcgg cccgctcccg tgccctcgca gtgatcaccg
      961 agatgatcga cgtgctcgac tacaccgagg caacggcggt gcatcatgcc cgcctgatcg
     1021 ccaacgcccg ccagtccgga aggcggcgcg cagcccacga catgatcatc gcagcccacg
     1081 ccgcacaaac cggccggacc ctgctgagca gggacgccgc ggcgcggttt ggcgacctgc
     1141 ccggcatcgc tgccgaaaac ccacccgtgt aagggctgtc ttggggtgac cgacggggct
     1201 cgaacccgcg accctcagta ccacaaactg atgctctacc aactgagcta cgaccaccat
     1261 gtcgctcccg caggagcatc cccagtgtgc caggtctgac ggcggattca cacctgattc
     1321 gggtactcgt ccgcggcggc ccgggacgac gaaatcggtc gtgtgctacc gtcgccggac
     1381 acgaatactt cgggagttga tatgagcgca actgattcct cgctcaccca ttctccgcag
     1441 atgcggaacc tcaccgagcg ggctcgcacc atcacccgcc gggaactggc catctacgcc
     1501 gagcgcaccc aggccagcca gcgggccacc gagcgggcca ggcaggtgct gccgctgggc
     1561 gtgccgtcca gctttcaggc ctacgacccc caccccatcg tggtccgcca ggcccaggac
     1621 tcgtggatgg aagacgtcga cggcaaccgc tacatcgact acgacatggg cttcggagcg
     1681 ttgttcgccg ggcattgtca ccccgcggtc cgccgggcga tcgaggccca actcgacatg
     1741 ggcacgttgt tcgtcacgcc gtgcgaggac aacgccacgg tcgccgagtt gctcggccag
     1801 cgctacgggc tgccgatgtg gcgcttcacc aacagcggca ccgaggccac catggacgcg
     1861 nnnnnnnnnn ccgtggcgtc acggggcgcc agaagctggt caaggtcgaa ggcgggtatc
     1921 acggccatca cgacgaggtg atgatctcga tgaagccgcc actcgaccag gccggtcctg
     1981 ccgatgcgcc gaacagcgtg ccgtccacgg ctggcatcac cgatgccgtg ttggccgaca
     2041 cgatcgtgat cccctacaac gaccccgatg cgctggaacg ggcgctgcgc ggtggcgacg
     2101 tggcctgctt catcgtcgag ccggtcatgg agaacatcgg catctgccgt cccgtgcccg
     2161 gctatctgga agcggtccgt gagatcacgg cgcgctacgg cacgctgctg atcttcgacg
     2221 acgtcaagac cggcatcacc gccgggtgga gcggggccac gggtgccttc ggtgtgctgc
     2281 ccgacctggt cacgctggcc aagagcatcg gcggcggtct gccgctcggc gccttcggtg
     2341 gcaagcgcga gtacatggac cagatcaccc agggccgcgt cctgcacctc ggcacctaca
     2401 acggcaaccc gctgtgcatg gccgccgcgg tcgccgtgct cgaccaggtg tgcannnnnn
     2461 nnnnctcgcc gacatctgca cccccgagac ccaacaggcc gtcaacgcgc tcaacggcaa
     2521 gctcgtcgcg gcgtgccaga acacgatcga cgccgccgga ctgccggcgc acaccgtgca
     2581 gttcggcgcc aagggctgtg tcacgtggtc ggtcgagccg atcaagaact accgcgacta
     2641 caaggccacc gacttcgacc tggccttcgc ccagtggatc cacggcatca atcgtggcgt
     2701 gctgttgccg cccggtctcg acgagcagtg gttgatctcg gtgctgcaca ccg
//
LOCUS       DAORNF010000453         2748 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_27847_length_2748_cov_1.781656, whole genome shotgun sequence.
ACCESSION   DAORNF010000453 DAORNF010000000
VERSION     DAORNF010000453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27847_length_2748_cov_1.781656"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2001)
                     /locus_tag="PLV68_15770"
     CDS             complement(<1..2001)
                     /locus_tag="PLV68_15770"
                     /inference="COORDINATES: protein motif:HMM:NF040143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HRE02758.1"
                     /translation="MTARVRLFGIRHHGPGSARAVQRTLAADPPQVVLIEGPSEADAI
                     VGLAGDPQMRPPVTLLGYVPDDLSRAMFFPFASFSPEWVAMRWALANHVPVHFVDLAL
                     RHTLATWPAEALVALPTAPDRPADPLAALAAAGGYDDAERWWEDVVEHRDGMSPFDAI
                     AEAMTVLRAAYEPDLVPAAPLERRREAQMRAGIRSAVATGHTDIAVVCGAWHVPALAD
                     ALDARVARLDAALLRGMPKAKVAITWVPWTHRRLASATGYGAGVSAPGWYHHLFTHPG
                     SDSVARWFTHGARLLRERGYSAPVADVVEATRLATSLAAVRGRPMPGLAEVSDAARAV
                     LGDGTDTPMVLLHDELVVGRQIGRVPDSTPMVPLARNLGAEQRACRLRPDGSITTLEL
                     DLRKPLDLRRSHLLHRLALLGIPWGTLTEGRGSTGTFRETWTMSWEPEYDVRLIEASA
                     LGTTVEVAAVAAVGQHAGEDAALARLTEAVEQCLAADLAAPVPGLVQAIAARVALDAD
                     VAHLLQALGPLARTLRYGDVRGTDAGALATVIRAMVGHIAAGLVPAFTDVDAEVAAEL
                     AVLVDDARSALSLLAQPEVTAVFHQALDAVAERDRIHPLLAGRATRLLADAGVLDPLA
                     QELRVSRALSVGVAASDGAFFIEGLLGGSGTVLVHDDDLLAVI"
     gene            complement(1998..>2502)
                     /locus_tag="PLV68_15775"
     CDS             complement(1998..>2502)
                     /locus_tag="PLV68_15775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRE02759.1"
                     /translation="PIPELGREVQARPGFNLIATANTRDRGVNELSSALRRRFNTVIL
                     PSPATVEDEVEIVVQRVADVAQSLALPAVEAPADEIQRVVTMFRELRNGASEDGRIRF
                     KTPSGTMSTAEAISVVVQGIAAAGHLGDGRLRFDDLLIGIESTVVKDPVHDTVVWREY
                     LDSYATR"
     assembly_gap    2503..2512
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2513..>2748)
                     /locus_tag="PLV68_15780"
     CDS             complement(<2513..>2748)
                     /locus_tag="PLV68_15780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRE02760.1"
                     /translation="AAAISGNSTLLVQGTAGTAEEALRYGWNYARLLAEGPSAAALVP
                     SPVFQAMEQGTLVRVEELTRLPSDVQDALITVLS"
BASE COUNT          434 a          970 c          969 g          365 t
ORIGIN      
        1 gatcacggcc aacagatcgt cgtcgtgcac caacacggtg cccgacccgc cgagcagacc
       61 ttcgatgaag aacgcgccgt cactggcagc gacgccgacc gacagcgccc gcgacacccg
      121 caactcctgg gccagcggat cgagcacccc ggcgtcggcc agcaaccggg tggctcggcc
      181 ggccagcaac gggtggatgc ggtcgcgttc ggcgaccgca tccaacgcct ggtggaacac
      241 ggcggtgacc tcgggttggg ccagcagcga cagcgccgag cgggcatcgt cgaccagcac
      301 cgccagttcg gccgcgacct cggcatcgac gtcggtgaac gcgggcacca acccggcggc
      361 gatgtgaccg accatggctc gaatcaccgt ggccaacgcg cccgcatcgg ttccgcgcac
      421 gtcgccgtag cgcaacgtgc gcgccaacgg tcccaatgcc tgcaacaggt gggccacgtc
      481 ggcgtcgagc gccactctgg cggcgatcgc ctggaccaga cccggcaccg gcgcggcgag
      541 gtcggccgcc aggcactgtt cgaccgcttc ggtcaacctg gccaaggcgg catcttcgcc
      601 agcgtgttgg ccgactgcag ccaccgcagc gacctcgacc gtggtgccca gggccgacgc
      661 ctcgatgagg cggacgtcgt attcgggctc ccacgacatg gtccaggtct cacggaacgt
      721 gccggtcgag ccacggccct cggtcagggt gccccacggg atgccgagca gcgccagacg
      781 gtggagcagg tgcgaccggc gcaggtcgag cggtttgcgc aggtcgagct cgagggtggt
      841 gatcgatccg tcgggccgca atcgacaggc acgctgttcg gcgccgaggt tgcgcgccag
      901 aggcaccatc ggtgtcgagt cgggcacgcg gccgatctgg cgaccgacca ccagttcgtc
      961 gtgcagcagc accatcggtg tgtcggtgcc gtcgcccaac accgcccggg cagcgtcgct
     1021 cacctcggcc aacccgggca tcggccggcc ccggactgct gccaggctgg tggccaaccg
     1081 tgtcgcttcg accacgtcgg cgaccggcgc gctgtagccg cgctcgcgca gcaggcgggc
     1141 accgtgggtg aaccaacgcg cgaccgaatc ggaacccggg tgcgtgaaca ggtggtgata
     1201 ccaaccgggc gccgagactc cagcgccgta gccggtcgcg ctggccaggc ggcgatgggt
     1261 ccacggcacc caggtgatcg cgaccttggc cttgggcatg ccccgcaaca gcgcggcatc
     1321 aaggcgggcc acgcgcgcat cgagcgcgtc ggccagcgcg ggcacgtgcc atgcgccgca
     1381 gacgacggcg atgtcggtgt gcccggtggc cacggcggag cggatgccgg cgcgcatctg
     1441 cgcttcgcgg cgacgttcga ggggggccgc cggaaccagg tccggctcgt acgcggcgcg
     1501 caacaccgtc atcgcctcgg cgatcgcatc gaacggcgac atcccgtcgc ggtgttcgac
     1561 gacgtcttcc caccaccgct cggcgtcgtc gtagccgccc gccgcagcca gagccgccaa
     1621 cggatcggcg gggcggtcgg gcgcggtggg cagcgcgacg agagcctcgg caggccacgt
     1681 ggccaaggtg tggcgcaggg ccaggtcgac gaagtgaacg ggaacgtggt tggccagcgc
     1741 ccagcgcatg gccacccact cggggctgaa cgatgcaaac ggaaagaaca ttgcccgact
     1801 caagtcgtcg ggtacgtagc cgagcagggt cacaggcgga cgcatctgcg gatcgccagc
     1861 cagtccgacg atggcgtcgg cctcgctcgg cccttcgatc agcaccacct gcggtggatc
     1921 ggcggcgagg gtccgctgca ccgctcgggc cgaccccggc ccgtggtggc ggatgccgaa
     1981 caagcggacc cgggcggtca tcgcgtggcg tacgaatcga ggtactcgcg ccagaccacg
     2041 gtgtcgtgca ccgggtcttt gaccacggtc gactcgatgc cgatgagcag gtcgtcgaag
     2101 cgcagccgcc cgtcaccgag atggccagcc gcagcgatgc cctgcaccac taccgagatc
     2161 gcctcggccg tgctcatggt gcccgatggc gtcttgaacc ggatgcggcc gtcttcgctg
     2221 gcgccgttgc gcagctcgcg gaacatggtc accacgcgct gaatctcgtc ggccggcgcc
     2281 tcgacggcgg gcagcgccag cgactgggcc acatcggcga cgcgctgcac cacgatctcg
     2341 acctcgtcct cgaccgtggc gggcgagggc aggatcacgg tgttgaaccg gcgccgcaac
     2401 gcactggaca gctcgttgac gcctcggtcg cgggtgttgg cggtggcgat caggttgaac
     2461 ccgggccgag cctgcacctc gcggccgagt tcggggatgg gtnnnnnnnn nncgctgagc
     2521 acggtgatca gcgcgtcctg cacgtcgctg ggcaaccggg tcagctcttc gacacgcacc
     2581 agcgtgccct gctccattgc ctggaagacc ggcgacggca ccaatgccgc agccgaggga
     2641 ccttcggcca acagccgggc gtagttccac ccgtagcgca gcgcctcttc ggcggtaccg
     2701 gcggtgcctt gcaccagcag cgtcgagttg ccgctgatcg ccgccgcg
//
LOCUS       DAORNF010000454         2741 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_27945_length_2741_cov_1.901713, whole genome shotgun sequence.
ACCESSION   DAORNF010000454 DAORNF010000000
VERSION     DAORNF010000454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2741
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27945_length_2741_cov_1.901713"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..728
                     /locus_tag="PLV68_15785"
     CDS             <1..728
                     /locus_tag="PLV68_15785"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRE02761.1"
                     /translation="WNQYYDAFVSDQFQHALRVTGKYALITVPAGLVLGVALAVLADK
                     WLRGVGFFRTVFSSTVATSVAVASVIWFFLLNKDVGVIAKWLGGTARTPGFLNDTGTA
                     LFAVSASSIWANLGFTFIVVTAALQSVPKDLYESAYVDGAGGWARFTNVTLPLISPTL
                     LFTTVVLTTRAFQSFGEIDLLTSGGPKGTTTTLTYLAYGKGMPTSGDDGLRATVSILI
                     FLVLLVLALAQFRGLEKRVHYGN"
     gene            718..1566
                     /locus_tag="PLV68_15790"
     CDS             718..1566
                     /locus_tag="PLV68_15790"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRE02762.1"
                     /translation="MATDAPTAVIDQVRGWRRVGRYALLVAMAVVVLFPIYVVVVAAF
                     KPTDKLLIDPLLPTDLVTSSFTKAWDFGSLGRTLFNSFVVSTLITLAQVITSVMAAYA
                     FVFLRFPAKSLVFGVFLATLLVPLEATITVNRRTMEDLGWINSYKALTVPFLATAFGV
                     FMIRQVFLQIPKEMREAAAMDGLGHWGFLREVAVPLSRPTIGALALFGFLSSWNQYLW
                     PVLVTTDERYNTVQTGLKSLKQGAFSQPNVLLAGTVIVALPIFVLLIAFQRQLVRGLT
                     AGAVKG"
     gene            1586..>2069
                     /locus_tag="PLV68_15795"
     CDS             1586..>2069
                     /locus_tag="PLV68_15795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02763.1"
                     /translation="MSDVNSSSRVRRARVRGVALLVTAAALAAACSSGSGDLSSAGNQ
                     TQTTQPTTTTAATSTTAASTDSTTAPDSSGDVASTTEATVAPTTTDSALLDSLPGCDT
                     LLLASASGPVEITFWHALSNENGAKLEEMVTRFNGSQNEVKVNIQFQGGYDENATKYL
                     Q"
     assembly_gap    2070..2140
                     /estimated_length=71
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2141..>2401
                     /locus_tag="PLV68_15800"
     CDS             <2141..>2401
                     /locus_tag="PLV68_15800"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRE02764.1"
                     /translation="PAEACIRSAGYDLSSFLQQPLDAYATQGIQWAMPFNMSVPVLFY
                     LRPDFVEAGLDPDSPPQNFDEFFAAAKQIVDSGAATYSVALD"
     assembly_gap    2402..2428
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2429..>2741
                     /locus_tag="PLV68_15805"
     CDS             <2429..>2741
                     /locus_tag="PLV68_15805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02765.1"
                     /translation="KGGHFYADNDNGRSGPATKVLFDEAAGVELMTYLQSLVTEANAV
                     YVGDNASGQDTLLQLANDTSPAAMAVSTSAALGTVMSVLGSGLIPGITTDDVGVGPMP
                     GP"
BASE COUNT          414 a          894 c          842 g          493 t
ORIGIN      
        1 gttggaacca gtactacgac gcgttcgtga gcgaccagtt ccagcacgcg ctgcgggtca
       61 ccggcaagta cgcgctgatc acggttcccg ccggtctggt gctgggcgtg gccttggcgg
      121 tcctggccga caagtggctg cgcggcgtcg ggttcttccg cacggtgttc tcgagcacgg
      181 tcgcaaccag cgtcgcagtc gcctcggtga tctggttctt cctgctcaac aaagacgtcg
      241 gcgtgatcgc caagtggttg ggtggcacgg cgcgcactcc ggggttcctc aacgacacgg
      301 gcaccgcgct gttcgccgtc tcggccagtt cgatctgggc caacctcggc ttcacgttca
      361 tcgtggtcac cgccgcgctg cagagcgtgc cgaaggatct atacgaaagc gcctacgtcg
      421 acggtgccgg tggctgggcg cggttcacca acgtgaccct gccgctgatc tcaccgacgc
      481 tgctgttcac cacggtggtg ctgacgacgc gggcgttcca gtcgttcggc gagatcgacc
      541 tgctcacctc gggcgggccg aagggcacga cgaccacgct cacctacctg gcctacggca
      601 agggcatgcc aaccagtggc gacgacggcc tgcgcgccac cgtgtcgatc ctgatcttcc
      661 tggtgctgtt ggtgttggct ctggcgcagt tccgcggcct ggagaagcgg gtgcactatg
      721 gcaactgatg ccccgaccgc ggtgatcgac caagtgcgtg gctggcgccg cgtcggccgc
      781 tacgcgctgt tggtggcgat ggcggtcgtg gtgttgttcc cgatctacgt cgtggtggtc
      841 gctgcgttca agccgaccga caagctgctc atcgacccgc tgctgccgac cgatctggtg
      901 acgtcgagct tcaccaaagc ctgggacttc ggcagcctcg ggcgcacgct gttcaactcg
      961 ttcgtggtgt ccacgctgat cacgctggcc caggtcatca cttcggtgat ggccgcgtac
     1021 gcgttcgtgt tcctgcgctt ccccgccaag tcgctggtgt tcggcgtgtt cctcgcgacc
     1081 ctgttggttc cgctcgaagc gaccatcacg gtcaaccggc gcaccatgga agacctcggt
     1141 tggatcaaca gctacaaggc cctgaccgtg ccgttcctgg ccaccgcgtt cggcgtgttc
     1201 atgatccgtc aggtattcct gcagatcccc aaggagatgc gcgaggcggc ggcgatggac
     1261 ggcctcggcc actgggggtt cctgcgcgag gtcgcggtgc cgctgtcgcg tcccaccatc
     1321 ggggcgttgg cgctgttcgg cttcctcagt tcgtggaacc agtacctgtg gcccgtgctg
     1381 gtcaccaccg acgagcgcta caacacggtg cagacgggac tgaagtcact gaaacagggt
     1441 gctttctcac aaccgaacgt gttgttggca ggcaccgtta tcgtcgccct cccgatcttc
     1501 gttctgttga tcgcgttcca acgccaactc gtgcgcggtc tcaccgccgg cgccgtgaag
     1561 ggatgaacct ccaatgtctc gtgtcatgtc tgatgtgaac tcctccagcc gggtgcgccg
     1621 cgctcgcgtg cgtggtgttg cgctgctggt gaccgccgct gcgctcgcag ccgcgtgcag
     1681 cagcggcagc ggcgatctca gctctgccgg caaccagacg cagacgacgc agcccaccac
     1741 gaccacggcc gccacgtcga ccaccgctgc cagcaccgac agcacgaccg cgcccgattc
     1801 cagtggcgat gtggcgagca ccaccgaggc cacggttgcg ccgaccacca ccgactcggc
     1861 cctgctcgac tcgctgcccg gctgcgacac cctgctgctg gcttcggcct ccgggccggt
     1921 cgagatcacc ttctggcacg cgctcagcaa cgagaacggc gccaagttgg aagagatggt
     1981 gacccgtttc aacggttcgc agaacgaggt caaggtcaac atccagttcc agggtggtta
     2041 cgacgagaac gccaccaagt acctgcagan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tgcctgccga ggcgtgcatt
     2161 cgctcggcgg gctacgacct gtcgtcgttc ttgcagcagc cgctcgacgc ctatgccacc
     2221 cagggcatcc agtgggcgat gccgttcaac atgagcgtgc ccgtactgtt ctacctgcgc
     2281 cccgactttg tcgaagccgg actcgacccc gactcgcctc cgcagaactt cgacgagttc
     2341 ttcgccgcgg cgaagcagat cgtcgactcg ggcgcggcga cctacagcgt cgccctcgac
     2401 annnnnnnnn nnnnnnnnnn nnnnnnnnca agggtggcca cttctacgcc gacaacgaca
     2461 acggccgcag cggcccggcg accaaggtgc tcttcgatga ggctgctggc gtcgagttga
     2521 tgacctacct ccagtcgctg gtcaccgagg ccaacgcggt ctacgtgggc gacaacgcga
     2581 gcgggcagga cacgttgctg cagttggcca acgacacgtc gcccgcagcg atggcggtgt
     2641 ccacctcggc cgcgctcggc accgtgatga gcgtgttggg cagcgggttg atccctggca
     2701 tcacgaccga cgacgtcggt gtcggcccga tgcccggacc c
//
LOCUS       DAORNF010000455         2741 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_27946_length_2741_cov_1.877141, whole genome shotgun sequence.
ACCESSION   DAORNF010000455 DAORNF010000000
VERSION     DAORNF010000455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2741
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27946_length_2741_cov_1.877141"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>602)
                     /locus_tag="PLV68_15810"
     CDS             complement(<1..>602)
                     /locus_tag="PLV68_15810"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HRE02766.1"
                     /translation="VLREGEDRAAFLARAAEALGFRDLWLGEHVAIPSRQRVGLYHDA
                     DIIRDDTDLYDVWTVCGAVLAVTDELVVAPGVLIAGLRHPVVTALAAATASAVGDGRF
                     RLGLGAGWLEEEFELFGIDFRSRFRRIEEVVAVVRALAAGGAAEFHGEFYDFAPVTTN
                     RSAASFPIIFGGLTEAAIARAARLGDGWYGVPGLSLDRHL"
     assembly_gap    603..612
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(622..>1505)
                     /locus_tag="PLV68_15815"
     CDS             complement(622..>1505)
                     /locus_tag="PLV68_15815"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRE02767.1"
                     /translation="RLGLDYESVAAVKPDLVYTEISAFGQTGPWRMMPAHGYNMMATS
                     RHLAVDWVDGRPEFGPNNGIRGNVAFGTTAALATLAALLQRTRTGKGQYLEVSLWDET
                     IHESMELADTMNGVAPMMVALDVANAPRYNVYRTADDQVIFIGPIERHFWVNFCRVVG
                     RDEWIDRARFEQPMDHGNYDTELRGLIQDVIATRPLGEWMDLFLANDVPASPVMLDDE
                     ALLNSAHLRERAMVVQASHPDYGTVHSFRPGFTLPGETFTIRHAPPMLGDHTAEILGE
                     LGIDGAELDRLHEDGVV"
     assembly_gap    1506..1546
                     /estimated_length=41
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1547..1805)
                     /locus_tag="PLV68_15820"
     CDS             complement(<1547..1805)
                     /locus_tag="PLV68_15820"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRE02768.1"
                     /translation="MYSLLNGFRVLDLTRLIHAGFVTQWLGDLGADVIKVEAPGVGDY
                     LRSLPPLRNGVSAGYLALNRNKRSLSLDLKHPRGREIFHRLL"
     assembly_gap    1921..1958
                     /estimated_length=38
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1959..>2353)
                     /locus_tag="PLV68_15825"
     CDS             complement(<1959..>2353)
                     /locus_tag="PLV68_15825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02769.1"
                     /translation="NVVDESGAVHRGYELDAQQFGTDDWHRLYATADGYICVSCNTET
                     ERGALATALGVQAGDDVAAQVAAALAGMTSDDAFALLDGAGVPCEIAVPDPQMPDLLW
                     DEWLAETSRVLEQHHPHWGWIREIGVLVH"
     assembly_gap    2354..2437
                     /estimated_length=84
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2438..2706)
                     /locus_tag="PLV68_15830"
     CDS             complement(<2438..2706)
                     /locus_tag="PLV68_15830"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRE02770.1"
                     /translation="MHNFRPGKAEPVGIGEQTLRAIRPDLVYCYLPGFGSSGPKSRLK
                     SFAPLLSGFVGNFFEGAGAGEPPVRRVIGNEDYYNGLGGAVAVLMG"
BASE COUNT          422 a          901 c          868 g          377 t
ORIGIN      
        1 cagatggcgg tcgaggctga gtccgggtac gccgtaccag ccgtcgccga ggcgcgcggc
       61 gcgggcgatc gccgcttccg tgagcccgcc gaagatgatc gggaaagacg ccgcgctgcg
      121 attggtggtg accggtgcga agtcgtagaa ctcgccgtgg aactctgccg caccgcccgc
      181 ggctagcgcg cggacgacgg cgacgacctc ttcgatgcgc cgaaagcggg agcggaagtc
      241 gatgccgaac agctcgaact cctcctccaa ccaaccggca cccagaccga ggcggaagcg
      301 gccgtcaccg acggcggacg cggtcgccgc agctagggct gtcacgaccg gatggcggag
      361 cccggcgatg agcacgccgg gtgccaccac caattcgtcg gtcaccgcca gcaccgcgcc
      421 gcacacggtc cacacgtcgt agagatcggt gtcgtcgcgg atgatgtccg cgtcgtggta
      481 caggcccacc cgctggcgcg acgggatggc gacgtgctcg ccgagccaca ggtcgcggaa
      541 ccccaacgcc tcggccgcgc gggccagaaa ggcggcgcgg tcctcgccct cgcgcagcac
      601 gannnnnnnn nnggtcggcg ctcacaccac tccgtcctcg tgcagccgat cgagctcggc
      661 gccatcgatc cccaactcgc ccaggatctc cgccgtgtgg tcaccgagca tcggcggcgc
      721 atggcggatc gtgaaggttt cacctggcag cgtgaaaccg ggacggaagc tgtgcacagt
      781 gccgtagtcg gggtggctgg cctgcaccac catcgcccgc tcgcgcaggt gagccgagtt
      841 gagcaacgcc tcgtcgtcga gcatcaccgg cgaggccggg acgtcgttgg ccaggaacag
      901 atccatccac tcccccaacg gacgggtcgc gatcacgtcc tggatcagcc cgcgcagctc
      961 ggtgtcgtag ttgccgtgat ccatcggctg ctcgaaccgg gcgcggtcga tccactcgtc
     1021 gcgcccgacc acgcggcaga agttgaccca gaagtgacgc tcgatcgggc cgatgaagat
     1081 cacctgatcg tcggcggtgc ggtacacgtt gtagcgcggc gcgttcgcga cgtcgagcgc
     1141 gaccatcatc ggggcgaccc cgttcatggt gtcggccaac tccatcgact cgtggatggt
     1201 ctcgtcccac aacgacacct cgagatactg ccccttcccc gtgcgcgtac gctgcaacag
     1261 ggcggcgagg gtggccagcg cggccgtcgt gccgaacgcg acgttgccac ggatgccgtt
     1321 gttggggccg aactcgggac gcccgtccac ccagtcgacc gccaggtgac gactcgtggc
     1381 catcatgttg tagccgtggg ccggcatcat ccgccacggt ccggtctggc cgaaggcgga
     1441 gatctcggtg tacaccaggt cgggcttgac ggcagccacc gactcgtagt ccaacccgag
     1501 gcgagnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnggag cagccgatgg
     1561 aagatctcgc gcccgcgcgg gtgcttgagg tcgaggctga ggctgcgctt gttgcggttg
     1621 agggcgaggt agccggcgct aacgccgttg cgcaacggcg gcaagctgcg caggtagtcg
     1681 ccgacaccgg gtgcttccac cttgatcacg tcggctccga ggtcgcccag ccactgcgtc
     1741 acgaacccgg cgtggatgag acgggtcaga tcgagcaccc ggaacccgtt gagcagcgag
     1801 tacacgggtc aggccacctc gctcgctgcc gcgcacgcgc ccgcggcgac gagcccgtcg
     1861 atcgcctcgc tgtcgtagcc caaatcaccc agcacctcgc gcgtgtgctc gccgagcagc
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnat gcaccagcac gccgatctcc
     1981 cggatccagc cccagtgcgg gtggtgctgc tcgagcacgc gtgaggtctc ggccagccac
     2041 tcgtcccaca acaggtcggg catctgcgga tcgggcacgg cgatctcgca cggcaccccg
     2101 gcgccgtcca agagggcgaa cgcgtcgtcg ctggtcatcc cggccagtgc tgctgccacc
     2161 tgggccgcga catcgtcacc ggcctgcaca cccaacgcgg tcgcgagtgc gccacgctcg
     2221 gtctcggtgt tgcaggagac gcagatgtag ccgtcggcgg tcgcgtagag ccgatgccag
     2281 tcgtcggtgc cgaactgctg ggcgtcgagt tcgtaacccc gatgcaccgc cccggactcg
     2341 tcgaccacgt tgtnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnccc atcagtacgg cgaccgcgcc
     2461 gccgaggccg ttgtagtagt cctcgttgcc gatcacccgg cgcacgggtg gctcgcccgc
     2521 tcccgcacct tcgaagaagt tgccgacgaa accggacagc agtggcgcga agctcttcaa
     2581 ccgcgacttg ggtcccgacg acccgaagcc cgggaggtag cagtagacga ggtcgggccg
     2641 gatcgcgcgc agcgtctgct cgccgatccc caccggttcg gccttgcccg ggcggaaatt
     2701 gtgcatcacc acgtcggcgg tcgcggccag gcggtgcacc a
//
LOCUS       DAORNF010000456         2732 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_28076_length_2732_cov_1.421741, whole genome shotgun sequence.
ACCESSION   DAORNF010000456 DAORNF010000000
VERSION     DAORNF010000456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28076_length_2732_cov_1.421741"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..640)
                     /locus_tag="PLV68_15835"
     CDS             complement(<1..640)
                     /locus_tag="PLV68_15835"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HRE02771.1"
                     /translation="MAFLPDGRALVTTKGGWGGRGEGEVLLLDQQGAFVDTLLTLPVC
                     ADAERGLLGITLDLRFSDNGRVFIFYTEGTETCDIREFGQSSDEPVYDVLMMFVFDND
                     RLGPDGVEYLRIDANEAAHRGGGLGAMPDGSILLSTGEAGGGIASQDPDSPHGKLLRI
                     HPDFPYQGFPDNPLYEPDDPTAIRSLVYASGLRNPFRFGIDQQTGTVLVTDVG"
     gene            1022..1708
                     /locus_tag="PLV68_15840"
     CDS             1022..1708
                     /locus_tag="PLV68_15840"
                     /inference="COORDINATES: protein motif:HMM:NF017453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02772.1"
                     /translation="MATGRHRVLMAIAAPTIDAYARRHGWDVVLSSEVLAPERPPSWA
                     KIRLLQDLMGHYEYVFWIDADAIVVDTRRNVLDEIDRAKQMWFASHPQQRDERAVVLN
                     AGVILLRSGEFAEHLLRSTWEQTQYVDHNWWENAALLALLGYSLESPYARLRDSEFDD
                     GIGALDLSWNSVPGYCESTAPAVNHHARADHDDFDRRVAAMAADLVSARNTFPSRLRR
                     AAWRRAVARI"
     gene            1751..2185
                     /locus_tag="PLV68_15845"
     CDS             1751..2185
                     /locus_tag="PLV68_15845"
                     /inference="COORDINATES: protein motif:HMM:NF017239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HRE02773.1"
                     /translation="MRHRIAHGEIAHERVDDEVIAVDLTTGAYYSLRGAGADTWDLLV
                     GGADEAEVVATLRARYEIIGGDVEADVDAFVRDLLAAGLLAGDDAVSEATRPPLPTLA
                     SGTTYTRPTLEAYDDMQGLLLLDPIHEVDEAGWPVIPVAPTD"
     gene            2191..>2732
                     /locus_tag="PLV68_15850"
     CDS             2191..>2732
                     /locus_tag="PLV68_15850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02774.1"
                     /translation="MRARDRVDAAAYLDALGDAFHRAGQLCGIEARHLDFAGYPVRVE
                     CAGSELAAKILPAFEHIAGSSPTADPVLTVRAWDRASAGEAPPPPPWKLDNTVQHRVE
                     IDPSVLRANYDAGFGLLSRRQPQSSQALFYGSSPAGMPRWVQRMPFRHILGWWAEETG
                     LSFVHAACVGRDGACVLLPG"
BASE COUNT          443 a          887 c          911 g          491 t
ORIGIN      
        1 tgccgacgtc ggtgaccagc acggttcccg tctgttggtc gatcccgaac cggaatgggt
       61 tgcgcagtcc cgacgcatag acgagtgagc ggattgcggt cgggtcgtcg ggttcgtaga
      121 ggggattgtc agggaagccc tgatagggaa agtcgggatg gatccgcaac agcttgccgt
      181 gcgggctgtc cgggtcttga ctcgcgatcc caccacctgc ttcacctgtg ctcagcagga
      241 ttgacccgtc tggcatcgcg cccagcccac cgccacgatg cgccgcctcg ttggcatcga
      301 tccgcagata ctcgacccca tcaggaccga ggcggtcgtt gtcgaagacg aacatcatca
      361 agacgtcgta gacgggctca tccgaggatt gtccgaattc gcggatgtcg cacgtctcgg
      421 tgccctcggt gtaaaagatg aacactcgcc cgttgtcgct gaagcggagg tcgagggtga
      481 tgcccaacag gccgcgctcg gcgtcggcac agaccggcag cgtgagcagt gtgtcgacga
      541 atgctccctg ctggtcgagc agcagcacct ctccttcgcc gcgtccaccc cagccaccct
      601 tggtcgtcac cagtgcccgg ccgtcgggca gaaaggccat gtctgtcgct gcgtactcgc
      661 ggtcgctcca gacctcggtt gtgaactgag cactgatgtc accgcgccac gtactccagg
      721 tatcgcggcg ctctccccag tcgggtccgt cgcttgcaca accagccgcc agcaccgcgc
      781 acgcaagcgc cgttgtcatc cctcgtctca acacgtagcg acaccttacc ctcggtcact
      841 cgcgcgatcc ggactttgtc gcggaccgcg ccgtctatcc cctggtcagg ggcagtggcg
      901 ctggaggggg ctcgaaactg ctaaacgtag cgagatgcgg gcgagcgggg tgcctttcga
      961 gggtcagcgg ctggtgttgt cacatcggcg tcggtcgagt ccgtcggcgg tgatctgctc
     1021 gatggcgacc ggtcgccacc gagtattgat ggcaatcgcg gcaccgacga tcgatgccta
     1081 cgcgcggcgc cacgggtggg acgtcgtgct gtccagcgag gtgctcgccc ccgagcgacc
     1141 gccatcgtgg gccaagatcc ggctgctgca ggatctcatg gggcactacg agtacgtgtt
     1201 ctggatcgac gcggacgcga tcgtggtcga tacccgccgg aacgtgctcg acgagatcga
     1261 tcgtgccaag cagatgtggt tcgcaagcca ccctcagcag cgagacgagc gtgccgtcgt
     1321 gctcaacgct ggcgtcatcc tgctgcgatc gggtgagttc gccgagcacc tcttgcgttc
     1381 gacgtgggag cagacccagt acgtcgatca caactggtgg gaaaacgcgg ccctgctggc
     1441 cttgctgggc tactcgttgg agtctcccta cgcgcgccta cgggactcgg agttcgacga
     1501 tgggatcggt gcactcgacc tcagttggaa cagcgtgccg ggctactgcg agagcacagc
     1561 gccagcggtc aaccatcatg ctcgcgccga tcatgacgac ttcgaccggc gtgttgccgc
     1621 gatggctgcc gacctggtct cagcccgcaa tactttcccg agcaggcttc ggagagcggc
     1681 gtggcgacgc gcggtcgctc gtatctgaac tgtcggcgga gatggatcgg gccagtaatg
     1741 tcctgatctc atgcggcaca gaatcgccca cggagagatc gctcacgagc gggtcgacga
     1801 cgaggtcatc gcggtcgacc tcacgaccgg cgcgtactac tcgctgcgcg gcgccggggc
     1861 cgacacctgg gacctactcg ttggcggcgc ggacgaggct gaggtcgtcg cgaccttgcg
     1921 ggcgcgctac gagatcatcg gcggcgatgt cgaggccgat gtcgacgcgt tcgtgcgcga
     1981 cctgctcgct gctgggttgc tcgccggtga cgacgccgtg agtgaagcca cgcgaccgcc
     2041 cttgcccacg ctcgcgagtg gcaccacgta cacacgtccg acgctcgagg cgtacgacga
     2101 catgcaaggc ctactcctgc tcgacccgat ccacgaggtt gacgaggcgg gttggccggt
     2161 catcccggtg gcgccgaccg actgacgctc atgcgtgccc gcgatcgtgt cgatgccgcg
     2221 gcgtatctcg acgcgctcgg cgacgcgttc caccgagcag gacagctctg tgggatcgag
     2281 gctcggcacc tggacttcgc cggctacccg gtccgcgtgg aatgtgccgg atcggagttg
     2341 gcagcgaaga tcctgccagc gttcgagcac atcgcggggt cgtccccgac cgccgacccg
     2401 gtgctcacgg tgcgcgcctg ggatcgcgcc agtgcagggg aagccccgcc acctccgccg
     2461 tggaagctgg ataacacagt gcagcatcgc gtcgagatcg atccatccgt gctccgggcc
     2521 aactatgacg cggggtttgg tctgctctct cggcgccagc cgcagtcgag ccaggcactg
     2581 ttctatggca gcagcccggc cggtatgccg aggtgggtgc agcgcatgcc gttccgccac
     2641 atcttgggat ggtgggccga agagaccgga ctgtcgttcg ttcacgcggc ctgcgtcggg
     2701 cgcgatggtg cgtgcgtcct gctgcccggt at
//
LOCUS       DAORNF010000457         2727 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_28152_length_2727_cov_1.587575, whole genome shotgun sequence.
ACCESSION   DAORNF010000457 DAORNF010000000
VERSION     DAORNF010000457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2727
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28152_length_2727_cov_1.587575"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>212)
                     /locus_tag="PLV68_15855"
     CDS             complement(<1..>212)
                     /locus_tag="PLV68_15855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase domain-containing
                     protein"
                     /protein_id="HRE02775.1"
                     /translation="GEVVFNTTLNGYQEVLTDPSYAGQIITFTYPHIGNYGVTLRDFE
                     SRRLFCRGVVVRELVRRASNHRSEAHL"
     assembly_gap    213..374
                     /estimated_length=162
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            645..>749
                     /locus_tag="PLV68_15860"
     CDS             645..>749
                     /locus_tag="PLV68_15860"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRE02776.1"
                     /translation="MAAGCTVVAKPSEVTPMTAYMLSKLCIEAGLPASA"
     assembly_gap    750..854
                     /estimated_length=105
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<855..>1062)
                     /gene="pyrC"
                     /locus_tag="PLV68_15865"
     CDS             complement(<855..>1062)
                     /gene="pyrC"
                     /locus_tag="PLV68_15865"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441605.1"
                     /note="catalyzes the formation of N-carbamoyl-L-aspartate
                     from (S)-dihydroorotate in pyrimidine biosynthesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HRE02777.1"
                     /translation="RDIELSRLTGAPVHFLHLSTARSVELVRQAKADGLRVTAEAAPH
                     HFTLTAELLSGFDPVYKVNPPLREL"
     assembly_gap    1063..1086
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1087..>1473)
                     /gene="pyrC"
                     /locus_tag="PLV68_15870"
     CDS             complement(<1087..>1473)
                     /gene="pyrC"
                     /locus_tag="PLV68_15870"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441605.1"
                     /note="catalyzes the formation of N-carbamoyl-L-aspartate
                     from (S)-dihydroorotate in pyrimidine biosynthesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HRE02778.1"
                     /translation="ALGGFTAIVAMPNTDPPHDSRAVVELVRNLGEAAGLCEVLPSGC
                     ITMGRAGEALAPYAELRDAGVTLFTDDGNGVQDPLLMRRALEYANELGITLAQHCEVS
                     RLTEGAVMHEGSCCSRLGLPGWPSLA"
     assembly_gap    1474..1496
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1497..1739)
                     /locus_tag="PLV68_15875"
     CDS             complement(<1497..1739)
                     /locus_tag="PLV68_15875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02779.1"
                     /translation="MNETQQRPGAATELLILGGTVVDRRGEQRADVRVRDGRVTEVAE
                     GLTADAGETVLDAAGCVVSPGFVDLHTHYDAQLFWDP"
     gene            complement(1736..2689)
                     /locus_tag="PLV68_15880"
     CDS             complement(1736..2689)
                     /locus_tag="PLV68_15880"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HRE02780.1"
                     /translation="MKHFRSIEELGPAGLTRLLDLTDHMAEVNRRPQPKVPALRGKTV
                     VSLFFEDSTRTRISFETAAKRLSADTMSFSVGTSSVNKGESLRDTVETVAAMGVDAFV
                     VRHKSSGVPWQIGRWTQASVVNAGDGWHEHPTQALLDCYTIRTELARREGFDGLHIAI
                     VGDIKHSRVARSDVAAFTMLGARVTLVAPRTLLPPSLDGWPVEVVSSIDDVVGEVDVL
                     YLLRMQRERMHEALVPSLREYTDRFGLTPARAARLREHALVMHPGPMNRGVEIAVDPA
                     ELPGAVIGHQVTNGVSVRMAVLFDLLGSGAGLGGGFDGRAE"
BASE COUNT          371 a          853 c          815 g          374 t
ORIGIN      
        1 agatgcgcct cggagcggtg gttgctggcc cggcgcacca gttcgcgcac gaccaccccc
       61 cggcagaaca accgccgcga ctcgaagtcg cgcaacgtga cgccatagtt gccgatgtgg
      121 ggataggtga aggtgatgat ctggccggcg tagctcggat cggtcagcac ctcctgatag
      181 ccgttgaggg tggtgttgaa caccacctcg ccnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnacggtg ttgtgcatga acatgcagaa tcgctttcgt gctcacagac
      421 ctggcgatca tcgctgcgcc cgaccgtcgg cgaccactgc cgcgccgcgg aacaccgtgt
      481 ggcggacacg accgcgcagc tcccgtccgg cgtacggcgt attgcggctc ttgctggcca
      541 actcggcgga acgcaccgtc cagcgctcgt cgggatcgaa caccgtcagg ttggcgaccg
      601 ctcccggctc gacgtcacac ccgtgacggt cggccacccc cgcaatcgcc gccggctgta
      661 cggtcgtcgc aaagccgagc gaggtcacgc cgatgaccgc atatatgctt tcgaaactct
      721 gcatcgaagc cggtttgccg gccagcgccn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      841 nnnnnnnnnn nnnncagctc gcgcaacggt gggttgacct tgtagaccgg atcgaagccg
      901 gacagcagtt cggcggtgag cgtgaagtgg tgcggcgccg cttcggcggt gacgcgcagg
      961 ccgtcggcct tggcctggcg caccagctcg acgctgcgtg cggtcgacag gtgcaaaaag
     1021 tgcacgggcg cgccggtgag gcggctgagc tcgatgtcgc ggnnnnnnnn nnnnnnnnnn
     1081 nnnnnntcgg ccagcgacgg ccagccgggc aggcccaggc gtgagcagca cgagccctcg
     1141 tgcatcaccg cgccctcggt gagacgactg acctcgcagt gctgcgccaa ggtgatgccc
     1201 agctcgttgg cgtattcgag cgcacggcgc atcagcagcg ggtcttgcac accgttgccg
     1261 tcgtcggtga acagggtgac acccgcgtcg cgcaactccg cgtagggggc caacgcctca
     1321 ccggcgcggc ccatggtgat gcacccactg ggcaacacct cgcacaaccc ggcggcctcg
     1381 ccgaggttgc gcaccagttc gaccacggcc cggctgtcgt gcggcggatc ggtgttgggc
     1441 atcgccacga tcgcggtgaa cccgccgagc gcgnnnnnnn nnnnnnnnnn nnnnnnggga
     1501 tcccagaaca gctgcgcgtc gtagtgcgtg tgcaggtcga cgaagcccgg tgacaccacg
     1561 cacccggcgg catcgagcac ggtctcgccg gcatcggccg tcaacccctc ggcgacctcg
     1621 gtgacacggc cgtcacggac gcgcacatcg gcgcgctgct cgccgcgccg gtcgacgacg
     1681 gtgccgccca ggatcaacag ctccgtcgct gcgccgggtc gctgttgcgt ctcgttcatt
     1741 ccgctctccc atcaaacccg ccgccgagcc cggcaccgct gccgagcaga tcgaacagca
     1801 ccgccatgcg gacgctgacc ccgttggtca cctggtggcc gatcaccgcg cccggcagtt
     1861 cggccgggtc gacggcgatc tcgacccccc ggttcatcgg gccggggtgc atcaccaacg
     1921 cgtgctcgcg caggcgcgcc gcacgggccg gggtgagacc gaagcggtcg gtgtactcgc
     1981 gcaggctcgg caccagcgcc tcgtgcatcc gttcgcgctg catccgcagc aggtagagca
     2041 cgtcgacctc accgacgacg tcgtcgatgc tggacacgac ctcgaccggc cagccatcca
     2101 acgacggcgg caacagcgtg cgcggggcga ccagggtgac ccgtgcgccc agcatggtga
     2161 acgcggcgac gtcgctgcgc gccacccggc tgtgcttgat gtcgccgacg atcgcgatgt
     2221 gcaacccgtc gaacccctcg cgccgggcca gttcggtgcg gatcgtgtag cagtcgagca
     2281 gggcttgggt ggggtgttcg tgccaaccgt cgccggcgtt gacgacgctg gcctgggtcc
     2341 agcgcccgat ctgccacggc acaccgctgc tcttgtggcg caccacgaac gcgtcgacgc
     2401 ccatcgcggc caccgtctcg accgtgtcgc gcaggctctc acccttgttg acgctgctgg
     2461 taccgacgct gaagctcatc gtgtcggcgc tgaggcgctt ggccgccgtc tcgaagctga
     2521 tccgggtgcg ggtgctgtct tcgaagaaca ggctgaccac ggtcttgccg cgcagggccg
     2581 gcaccttcgg ctgcggacgg cggttgacct cggccatgtg gtcggtgaga tcgagcaagc
     2641 gggtgagccc cgctggtccg agctcctcga tcgagcggaa gtgcttcacg acctctcccc
     2701 cgcgggatcc ccacccggca cgtcctg
//
LOCUS       DAORNF010000458         2726 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_28166_length_2726_cov_1.736428, whole genome shotgun sequence.
ACCESSION   DAORNF010000458 DAORNF010000000
VERSION     DAORNF010000458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28166_length_2726_cov_1.736428"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    267..370
                     /estimated_length=104
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<371..>630)
                     /locus_tag="PLV68_15885"
     CDS             complement(<371..>630)
                     /locus_tag="PLV68_15885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02781.1"
                     /translation="LLRPLWSGTIVATANLVVGSLLHSILVGGSMQRSVLIGALFAAV
                     AQSAFWLLYYPCRGPLWRITQRDLDVLAARVEAARRRATSKG"
     assembly_gap    631..814
                     /estimated_length=184
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<815..1011)
                     /locus_tag="PLV68_15890"
     CDS             complement(<815..1011)
                     /locus_tag="PLV68_15890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02782.1"
                     /translation="MRAGLDQVCVSKAALTFFPTFRKEFTIDREDVDRIVVRDVIIIF
                     GSSTEYKIVLTNGSTYRWHFRA"
     assembly_gap    1457..1614
                     /estimated_length=158
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1875..2615
                     /locus_tag="PLV68_15895"
     CDS             1875..2615
                     /locus_tag="PLV68_15895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02783.1"
                     /translation="MADAPRGGTPRPFAFDGELVGEEPVPIEPGAVLTEPPVEAIVPK
                     SLDDPVEGDLQAPAPSEGSFDPEWAVELPGERDRFRSVYENPDGTRSAVLTQEAQNFV
                     AADGRWERIDPRLVPVKGQRDVWRTAASSVEVTVSAAGVSVRGEDGKAVSVRPGGAGV
                     QLHAPVVSADGLTATFAEVWPGVDVRFVVSNASVRKEIVVKRADVTPTFQMAVSGVEL
                     VVPPHFAIRFGWPLPARRQRNRRDAVAR"
BASE COUNT          385 a          713 c          794 g          388 t
ORIGIN      
        1 tgatgagatg caacctcatt tttcatcaca attcgagatg tcacgaccct ctggcatcgc
       61 tataggagat aagggcgggg tgcggagcct cccaggactc gcaatcgcga ctgcatcacc
      121 gggagcggtt gcttcgcgct cgtcggtgcc atggctgaat gacatgagac gatgtcctca
      181 agtcatgccc atcgccgaac ccagggtgag tccgcccgta gctgtggggt ggtagcgccg
      241 gagtgcgctg tggcagcgag gagagtnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn cgcccttgct ggtggcgcgg cgccgggccg cttcgacccg ggcggcgagc
      421 acgtcgaggt cgcgttgcgt gatccgccac agtggcccgc ggcacgggta gtacagcaac
      481 cagaacgccg actgggcgac cgccgcgaag agcgcgccga tcagaaccga acgctgcatc
      541 gagccgccca ccaggatcga gtgcagcagc gagccgacga ccaggttcgc cgtcgcaacg
      601 atcgttcccg accacagcgg acgcagcaac nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngcccgg aagtgccacc gatacgtcga
      841 cccgttcgtg agcacgatct tgtactcggt ggaggagccg aaaataatga tcacatcgcg
      901 aacgacaatc ctgtcgacat cctcacgatc gatcgtgaac tccttgcgga acgtcgggaa
      961 gaacgtgagc gcggccttcg acacgcacac ttggtcaagt ccggcccgca acgagaacga
     1021 ccaacgtatg accgcgcccc gaaagcagag gctcactcag cccacccccg tcccacgcgg
     1081 ccggcgcccg acttgtccgt gtccgactgc cctggacagg gggcagacca agcgcccgca
     1141 gcgggatgaa tcgagccacg cgttcgtgct ccggtgctga cgcagaagca cgtcggcgtc
     1201 caaccgtcgt cgaacgtgcc gtcgaccgat tcgtcaacga ggcagccgga ttgggaacgg
     1261 atcgccactc gacgtggtca gcgtcttcgg gccgttgcgc cgccactgca cactcgccac
     1321 ggtacccgcc gcaccctcag cgaatgcccg ttctcgcggt ggcccgacga gccaagcctc
     1381 gtggccctga cgcaggtttg agagacggcg cctgatcgtc gcggtggccc gacgagtcaa
     1441 acgtcgcgac cacggcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnactctt
     1621 cacgtcggct tatctgcatg gcgcgtccta cgtcatgccc gtgctactgg cacaggaagt
     1681 gacgtgatcg aagcaattcg agttggcact tgacgcgcag tgcaattatc gcgatactgc
     1741 acgcgatcat cggtcggggg gccggggtgt gagtggaggt cgttgccgtg ggtactcgca
     1801 cacatcgtgt tggctggttg ggcctgttgt cggtcctgtt gggcggctcg ttatcgtggg
     1861 ccgccccgcc ggtcgtggca gacgcgccac ggggtggcac accgcgcccg ttcgctttcg
     1921 acggcgagtt ggtgggggaa gagccagtcc cgatcgagcc aggtgcggtg ctgaccgagc
     1981 cgccggtgga ggcgatcgtg ccgaagtcgc tggacgatcc cgtcgagggc gacctgcagg
     2041 ctccggcacc ctcggagggc agcttcgatc ccgagtgggc ggtggagttg ccaggtgaac
     2101 gggatcggtt ccgatcggtg tacgagaacc cggacggcac tcgttcggcg gtgctgaccc
     2161 aggaggcgca gaacttcgtg gcggccgacg ggaggtggga gcggatcgac ccccgcctcg
     2221 tcccggtcaa ggggcaacgg gacgtgtggc ggaccgcggc gtcgtcggtg gaggtgacgg
     2281 tgtcggcggc gggcgtgtcg gtacgcggcg aggacggcaa ggccgtgtcg gttcgccctg
     2341 ggggtgccgg ggtgcaactc catgcgccgg tggtctccgc tgacgggttg accgcaacgt
     2401 ttgccgaggt atggccgggg gtcgacgtgc gtttcgtggt gtccaacgcg tcggtgcgca
     2461 aggagatcgt ggtcaagcgg gcagacgtga cgccgacgtt ccagatggcc gtgagcggtg
     2521 tcgagctcgt ggtaccaccc cacttcgcca tccggtttgg atggcctttg cctgcacggc
     2581 gccagcgcaa tcggcgcgat gcagtggctc gctaacggga gccgattgat tccttgcgga
     2641 atcccgtcac cgtctactga cgcccggagg cgcgttcggg gtgaagctcg ggggtcttat
     2701 cgatccggcg atcccgatcc cgcttt
//
LOCUS       DAORNF010000459         2726 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_28168_length_2726_cov_1.606889, whole genome shotgun sequence.
ACCESSION   DAORNF010000459 DAORNF010000000
VERSION     DAORNF010000459.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28168_length_2726_cov_1.606889"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>824)
                     /locus_tag="PLV68_15900"
     CDS             complement(<1..>824)
                     /locus_tag="PLV68_15900"
                     /inference="COORDINATES: protein motif:HMM:NF017411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02784.1"
                     /translation="VRDLPTVVARGASATRYRYDGFGRLVAKSDPDAGAWSYSYDANS
                     NPITVVDAKHQTTVTAFDILNRPTRRTVDGATVATWTYDTTAIGLLGASASIQPDGTY
                     TTTFDAYDPMNRTVAQTVTMPNGITSTTTQHFDRAGHAVDRTLTDSTGLTQPETVVST
                     YSDLGLPKTVSGASAYVTATGYDGLARVTSRTYASTSGIVRNYTYDARNRLATITQSV
                     NGAAVVDDTWTYDAAGNVTAIDSDTAVGQKQCFSLDALFRLIHAYTTATGADCALG"
     assembly_gap    825..902
                     /estimated_length=78
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(905..>2157)
                     /locus_tag="PLV68_15905"
     CDS             complement(905..>2157)
                     /locus_tag="PLV68_15905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02785.1"
                     /translation="FNGTEEVTGTITEYRLVRHTPGAPTGLFQADDNSARMLLPHRIT
                     GRAATGADRYAAVTPVETDFDPATGLPVTVAESGDGPANARCTRTDYTGTVDPAALVW
                     DRVTRTRLYAGACDTGVAVSETSATYDAAGNPLTATMHDGASSITTSLGYASDPYHRP
                     TSSTDGRGNTTTVKYLINTATGFATDIEGTNAAGHTVTIRTDPARGSVLSTTNARGFV
                     SRSTYDPLGRMIAGQTPSESGTALQSVEYRYLFDQPRFHPDGSTVVDVGTTANSPERY
                     LHDLNMVETRTLLRSAGEQGATRDTYTYAYAYIDGLGRTIEVQTASPAGGRVVAPAVF
                     DDRGLTARAIDPYHAAGLAGSGPLNGALPNPAAHEVQTVFDGAGRATTAIELVAGSEV
                     ARTTMAYTARSTTTWRCRSTVTMW"
     assembly_gap    2158..2167
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2168..>2726)
                     /locus_tag="PLV68_15910"
     CDS             complement(<2168..>2726)
                     /locus_tag="PLV68_15910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02786.1"
                     /translation="NLFRVDTIRDELGGEVAVTYGQPNGCIRNNWATNASNCCPGWWS
                     PKQGTAGFVAWNKYLVTEVVQGALSSASTFTNSVYVGEPITTRYSYVGDPAWHYDDRP
                     GVSKRVQSWGDWRGYETVRTSTGNSTPTVTETRYFRGMNGDRANASGGVRSVSVTASG
                     GITAEAPVFTAVDDPAPSCGGRRARS"
BASE COUNT          391 a          836 c          941 g          470 t
ORIGIN      
        1 cacccaacgc acagtcggcg ccggttgcgg tcgtgtaggc atggatcaag cggaacagcg
       61 cgtcgagcga gaagcactgc ttctggccca ccgccgtatc ggagtcgatc gcggtgacgt
      121 tgcccgccgc gtcatacgtc cacgtgtcgt cgaccacggc agcgccgttg acggactggg
      181 tgatcgtggc gagccgattg cgggcgtcgt aggtgtagtt gcgcacgatc ccactcgtgc
      241 ttgcatacgt gcgcgacgtg actcgggcca ggccgtcgta gcccgtcgca gtcacgtatg
      301 cgctggcgcc ggagaccgtc ttcggcaggc ccaggtcgct gtacgtggag acgaccgtct
      361 cgggttgggt caacccggtg ctgtccgtca gggtccggtc gaccgcgtgc ccagcccgat
      421 cgaagtgctg cgtcgtggtc gaggtgatcc cgttcggcat cgtgacggtc tgtgcgacgg
      481 tccggttcat cgggtcgtac gcgtcgaacg tcgtcgtgta ggtgccgtcg ggctggatcg
      541 atgccgatgc gcccagcagc ccgatcgccg tggtgtcgta ggtccacgtc gcaaccgttg
      601 caccgtcgac cgtgcgacgg gtcgggcggt tgaggatgtc gaacgcggtg acggtcgtct
      661 ggtgcttggc gtcgaccaca gtgatcgggt tggagttggc gtcgtagctg tagctccatg
      721 ccccggcatc gggatcggac ttggcgacga gacggccaaa cccgtcgtat cggtagcgcg
      781 tcgcgctcgc gccgcgcgcg acgacggtgg gaaggtcgcg gacgnnnnnn nnnnnnnnnn
      841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      901 nnagctacca catcgtcacg gtggaccggc agcgccacgt ggtggtggag cgcgcggtgt
      961 aggccatcgt cgtacgggcc acctcgctgc ctgcgaccaa ctcgatcgcc gtggtcgcgc
     1021 gcccggcacc gtcgaatacg gtctgcacct cgtgcgccgc cgggttgggg agggcaccgt
     1081 tgagcggccc cgaaccagcc aaaccggcgg cgtggtaggg gtcgatcgcg cgtgcggtca
     1141 gacctcggtc gtcgaacacg gctggcgcga cgacacggcc acccgcgggc gaggccgtct
     1201 ggacttcgat cgtgcggccg agcccgtcga tgtaggcgta tgcgtacgtg tacgtatcgc
     1261 gggtggcgcc ctgctctccg gcggagcgca gcagcgtgcg tgtctcgacc atgttgaggt
     1321 cgtggagata gcgctcgggc gagttggccg tcgtacccac gtcgaccacc gtgctgccgt
     1381 cgggatggaa ccgcggttgg tcgaacaggt agcggtactc cacgctctgc aacgccgtgc
     1441 ccgactcgct cggggtctga ccggcgatca tccgcccgag cgggtcgtac gtggaccgcg
     1501 acacgaagcc gcgagcgttc gtggtcgaca acaccgaccc gcgggcagga tcggtgcgga
     1561 tcgtgaccgt gtgtcccgca gcgttcgtgc cctcgatgtc ggtcgcgaac ccggtggcag
     1621 tgttgatcag gtacttgacg gtggtggtgt tgccacggcc gtcggtggag ctggtcggac
     1681 ggtgatacgg gtccgacgcg tagccgagcg acgtggtgat cgaggaagct ccgtcatgca
     1741 tggtcgccgt caacgggttg cctgccgcgt cgtacgtggc ggacgtttcg gacacagcga
     1801 cgccggtgtc gcacgctccc gcgtacaggc gggtccgggt cacgcggtcc cacaccagcg
     1861 cagcgggatc gacagtaccg gtgtagtcgg tacgcgtaca gcgggcattc gctgggccgt
     1921 cacccgactc ggccacggtg accggcaagc cggtcgcagg atcgaagtcg gtctcgacgg
     1981 gcgtcaccgc tgcataccga tccgctccgg tcgctgcccg cccggtgatc cggtgcggca
     2041 acaacatccg tgcgctgttg tcatccgctt ggaacaaccc ggttggagcg cccggcgtgt
     2101 ggcgaacgag gcggtactcg gtgatcgtgc cggtcacctc ctcggtgccg ttgaacgnnn
     2161 nnnnnnnaga ccgagcgcgc ctaccgccgc atgaaggcgc tggatcgtcg acggccgtga
     2221 acactggtgc ctcagcggtg atgccaccgg atgcggtcac gctgaccgag cgaacaccac
     2281 cgctggcgtt ggcgcgatcg ccgttcatgc cgcggaagta gcgggtctcg gtgacggtcg
     2341 gcgtggagtt ccctgtgctg gtgcgtacgg tttcgtagcc acgccaatca ccccaggact
     2401 gcacccgctt cgacacacct ggtcggtcgt cgtagtgcca tgccgggtct ccgacgtagc
     2461 tgtagcgggt ggtgatcggc tcacccacgt acacggagtt ggtgaacgtc gacgctgacg
     2521 acagagcacc ctggacgacc tcggtgacga ggtacttgtt ccaggccacg aaccctgcgg
     2581 tgccctgctt gggcgaccac caaccggggc agcagttcga cgcgttggtg gcccagttgt
     2641 tccggatgca accattcggc tggccgtacg tcactgcgac ctcgccgccg agttcgtcac
     2701 gaatcgtgtc gacccggaac aggttg
//
LOCUS       DAORNF010000460         2724 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_28195_length_2724_cov_1.442113, whole genome shotgun sequence.
ACCESSION   DAORNF010000460 DAORNF010000000
VERSION     DAORNF010000460.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2724
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28195_length_2724_cov_1.442113"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    248..271
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <272..>658
                     /locus_tag="PLV68_15915"
     CDS             <272..>658
                     /locus_tag="PLV68_15915"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HRE02787.1"
                     /translation="VLAGFGSSIVVVRQPQMGQPAAQNTALAYATGELVAFQDADDIW
                     PVGRLALMIDALADGIDGVYGALEQFVSPELDPASLGHVRIVQSDRPVQLWATSLIRR
                     PSLDRVGALDTATRTSSVFDWMSRAEA"
     assembly_gap    659..689
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            830..>1017
                     /locus_tag="PLV68_15920"
     CDS             830..>1017
                     /locus_tag="PLV68_15920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02788.1"
                     /translation="MIDADERLLVRALLDRDAATAAAATVEYLDRVDVQTLPWRQTRM
                     FPALFRRMEALGLQPPSL"
     assembly_gap    1018..1092
                     /estimated_length=75
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1093..>1396
                     /locus_tag="PLV68_15925"
     CDS             <1093..>1396
                     /locus_tag="PLV68_15925"
                     /inference="COORDINATES: protein motif:HMM:NF026257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HRE02789.1"
                     /translation="RLDQAGIGNVVLKGASLIPAYGGDWGVRDMADVDLLVDVADVPT
                     AIDLLAFDGYVPHLGGSKAAVLARCVPRRHSWNFTHASGANIDLHWRVLQHSCGRAA"
     assembly_gap    1397..1484
                     /estimated_length=88
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1485..2407
                     /locus_tag="PLV68_15930"
     CDS             <1485..2407
                     /locus_tag="PLV68_15930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02790.1"
                     /translation="LLLHVLEHAAHEEDESAVLWIIDAVHIVRASGDPEAMTARFAEQ
                     ARAHGLVDTMRAKLETIRDVTTEPRIELAAAGLTKWRVRPQPPAGSRRQRLAEHRRGG
                     AGWVETVASFVADGVDGALARRPRAWAAYVLTGRRPRVEQWLTARGGPLTTTPHSAAP
                     PVGEDGWWDVSANDVCDSLCGPGWSYAEPNERIVWTDGREARLSVPVAGRGPFVVDIE
                     CVVLGRHGGSPRRVALRAGGATVQTLVADQDDWLHHVSTPALVASSVGTVELSLLVDS
                     PARPIDLGLGADWRRLGLRLGRVRVRPTGS"
     gene            2413..>2724
                     /locus_tag="PLV68_15935"
     CDS             2413..>2724
                     /locus_tag="PLV68_15935"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRE02791.1"
                     /translation="MKILMVSGQFPPAIGGIEVLVAQLAAELVRRGHDVAVLTGTLRG
                     VSAGDGFHGDVRVHRNDLVRALIQRDPLAIARCSAALGGSRNTTRTRPSASMMHARPL
                     PT"
BASE COUNT          362 a          809 c          891 g          444 t
ORIGIN      
        1 cgacgctggc gcggctggcg caactcgctc gcagactccc ggtcgcgcgc atcgaactcg
       61 ggcgcgactt cgccgagatc acagggaccc tcggtgacct gatcgaacgg tggtcgacgt
      121 gaccacctcc ccgacggtca gcgcgctcat tccggtctac aacgcagacc ggtacatcgc
      181 tcagtcgatc gagtctgtgc tcgctcagcc cgaggttggt caggtcatcg tggtcgatga
      241 tggctcgnnn nnnnnnnnnn nnnnnnnnnn ngtcttggct gggttcgggt cgtcgatcgt
      301 ggtggtccgg caaccgcaga tgggtcagcc ggcggcgcag aacacggcgc tggcgtatgc
      361 cactggcgag ttggtcgcct ttcaggacgc ggacgacatc tggccggtcg gacgtttggc
      421 gctgatgatc gatgcgctcg cggacggtat cgatggggtc tacggggctt tggaacagtt
      481 cgtcagccca gagttggacc cggcgtcgct tggccacgtc cgcatcgtgc agtccgatcg
      541 tccagtacag ctgtgggcga cgtcgttgat tcggcgcccg tcactcgacc gcgtcggagc
      601 gctggatacc gccactcgaa cgagttcggt cttcgactgg atgtcccgtg ccgaggcann
      661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc agatcgtact gcgccgaaga attcacgacg
      721 cgaacctggg ccgccgggtc ggagcgcagg cgcgcaacgc cgacctcctg gccgtgttgc
      781 gctcgcacct cgctcgcagc cgcggccacg gcgaccaggc gaaccagccg tgatcgacgc
      841 tgacgaacgg ctcctcgtgc gggcattgct cgatcgcgat gcggcgacgg ccgcggctgc
      901 gacggttgag tatctcgacc gtgtcgacgt gcagaccctg ccgtggcgcc aaacacgaat
      961 gtttccggcg ctgttccgcc ggatggaggc gctcgggcta caaccgccga gtctgttnnn
     1021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nncccgcctc gaccaggccg ggatcggcaa cgtggtgctc aagggtgcgt
     1141 cactcatccc ggcttacggc ggcgactggg gtgtgcgtga catggccgac gtcgacctgc
     1201 tggtcgatgt tgccgatgtg ccaacagcaa tcgatctgct cgcgttcgac ggatacgtac
     1261 cgcacctcgg cggatcgaag gccgcggtgt tggctcgatg cgtgccgcgt cgacacagct
     1321 ggaacttcac ccatgcgtca ggcgccaaca tcgatctgca ctggcgggtg ctgcagcact
     1381 cgtgcggtcg ggcggcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnacttgc tgctccatgt
     1501 gctcgaacac gcggcgcacg aggaggatga gtcagcggtg ctgtggatca tcgatgcggt
     1561 gcacatcgtc cgcgcgagcg gcgaccccga ggcgatgacc gctcgcttcg ccgaacaggc
     1621 ccgcgctcat ggcttggtcg acaccatgcg cgccaagctg gagacgatcc gggacgtgac
     1681 gaccgagccc cgcatcgagc tggcagctgc tggactgacg aagtggcgcg tccgccctca
     1741 gccgcctgcc gggtctcggc gacagcgcct ggccgagcat cgccggggcg gcgcgggttg
     1801 ggtggagacg gtggcttcgt tcgttgccga cggtgttgac ggtgccctcg cccggcggcc
     1861 gcgggcctgg gccgcgtacg tgctgaccgg tcggcgaccg cgggtcgagc agtggctgac
     1921 agcgcgaggc ggcccactga cgaccacacc ccactcggct gcaccgccgg ttggggagga
     1981 cggctggtgg gacgtgtccg ccaacgatgt ttgcgactcg ctgtgcgggc caggttggtc
     2041 gtacgccgag ccaaacgagc gcatcgtgtg gaccgatggg cgcgaggccc gcctcagcgt
     2101 gcccgttgcg gggagaggac cgttcgtagt cgacatcgag tgtgtcgtgc ttggtcgcca
     2161 cggtgggtca ccgaggcggg tggcgctgcg cgccggtggt gccacagtgc agacactggt
     2221 cgccgaccag gatgactggc tgcaccacgt cagtacaccc gccctggtcg ccagcagtgt
     2281 cggcaccgtc gagttgtcgc tccttgtcga ctctccagcc cgaccgatcg atctgggtct
     2341 cggcgcggac tggcgccgtt tggggctgcg cctcggccgt gttcgcgtcc gcccaacggg
     2401 ttcgtgagtg gcatgaagat cctgatggtc tcgggccagt ttcctcctgc gatcggtggg
     2461 atcgaggtcc tcgtcgccca gttggcggct gaactcgtcc ggcgtgggca cgacgtcgcg
     2521 gtgctcaccg gaacgctgcg tggcgtctcg gctggggatg gattccacgg ggatgtgcgc
     2581 gtgcatcgca acgatctggt tcgtgcgctg atccagcggg atccgctggc gatcgcccgg
     2641 tgcagcgcgg cattgggggg atcgcgaaac acgacccgga cgcgtccgtc cgcgtcgatg
     2701 atgcacgcga ggcccctgcc aacc
//
LOCUS       DAORNF010000461         2723 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_28210_length_2723_cov_2.038981, whole genome shotgun sequence.
ACCESSION   DAORNF010000461 DAORNF010000000
VERSION     DAORNF010000461.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2723
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28210_length_2723_cov_2.038981"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /locus_tag="PLV68_15940"
     CDS             <1..561
                     /locus_tag="PLV68_15940"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HRE02792.1"
                     /translation="RPLTDDEVKEMSYLMIQGGFDTTGSAISNALIRLSSDRVARQRL
                     IDEPGLMGSAVEEFLRIEAPQFAMARTANVDVTIGGQEIKAGDKILMVWASANRDKTV
                     FDDPDELRLDRFPNRHMTFGLGAHRCLGSNLARRQIEVALRAVLQRMPDYEIDLDAAT
                     RPETIGIAYGMFALPATYTPGPRWFR"
     gene            581..1714
                     /locus_tag="PLV68_15945"
     CDS             581..1714
                     /locus_tag="PLV68_15945"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HRE02793.1"
                     /translation="MRVARIEAFPIDLQYRQDKEFRVSYGVIDSMQNAIVRVTTDDGL
                     VGYGELMPLPLGDRAACIKELQGIGPSVLGQDPFDIREIHARMDDALGVEIGSRQSLL
                     KGSIDFALHDIMGRAAGVPVYRILGGNYDDGVPMHFTISIRSPEEMGEDTAARVAEGF
                     RTIEVKLGRFEGELDLDTEIARIAAIREAAGPDVVIIADPNIGWTTQEAIDVLPSMEQ
                     FNVYVEQPVKGIEALGQVRAAISSPVIADESAANARVLAEIIRREAADLINLKILRTG
                     GLYEASKMLDICESFGIGYRHDNVIQSRLASTMMIHFSTTYNRGLPDGGGTQFIRTVQ
                     DVVNPDQGVYLDGGVAKLRDPDAPGLGATPREELLRDPIVIAG"
     gene            complement(1739..>2723)
                     /locus_tag="PLV68_15950"
     CDS             complement(1739..>2723)
                     /locus_tag="PLV68_15950"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRE02794.1"
                     /translation="GAALTALAEGLDAPVVTTIEGRGAISERHELALGAQMDMAAMDS
                     VFAQADVVLAVGTRFQQYSNVLKWLRFPGRLIHLDADPAVIGRVHPADVALVGDARLG
                     LDQLLAAVGDGRATHPDWVASCQRTRDEVVAQLRVDIGPDMDAIVDAICSTLPDDAIV
                     VKDATISAYLWANRIVPVTQTRSSMRPTSQAIGPGLPLAVGAATTGRTTVVVQGDGGL
                     MLSVGELATAAQYDLALILCVFNDRGYGMLRFIQDMAVAGRRTGVELATPDFVALGTA
                     FGVRSAHVATPAEFAAAFSAAVAAGGTWVLDVDITSMAPMVITPQARPVRS"
BASE COUNT          493 a          921 c          891 g          418 t
ORIGIN      
        1 cgcccgctga ccgacgacga ggtcaaggag atgtcctatc tgatgattca gggtggcttc
       61 gacaccaccg gctcggcaat ctcgaatgcg ctgatccgcc tgagcagcga tcgcgtggca
      121 cgccagcgcc tcatcgacga gcccggtctg atgggctcgg ccgtcgagga attccttcgg
      181 atcgaggcac cgcagttcgc gatggcgcgg accgcgaacg tcgatgtgac catcggtggc
      241 caagagatca aggccggtga caagatcctg atggtctggg cctctgccaa ccgcgacaaa
      301 acggtgttcg acgaccccga cgaactgcgc ctcgaccgat tccccaaccg ccacatgacc
      361 ttcggactcg gagcgcaccg ctgccttggc tcgaatctgg cgcgtcgcca gatcgaagtc
      421 gcgctcagag ccgtgttgca gcggatgccc gactacgaga tcgacctcga cgcagccacc
      481 cgacccgaga ccatcggcat cgcctacggg atgttcgctc ttcctgccac ctacacgccc
      541 ggaccgagat ggttccgctg atccagatgg agtcacacgc atgagagtcg cccgcatcga
      601 agcgtttccg atcgacctgc agtaccgcca ggacaaggag ttccgcgtgt cctacggagt
      661 gatcgactcg atgcagaacg cgatcgtgcg cgtcaccacc gacgacggtc ttgtcggata
      721 cggcgaactc atgccactgc cactcggtga tcgggccgca tgcatcaagg agctgcaagg
      781 gatcggcccg agcgtgctcg gtcaggatcc gttcgacatc cgcgagattc acgccaggat
      841 ggacgatgcg ctcggcgtcg agatcggcag ccgtcagtcg ctgctgaagg gcagcatcga
      901 cttcgcgctc cacgacatca tgggacgcgc cgccggtgtc ccggtgtacc ggatcctcgg
      961 cggcaactac gacgacgggg tgccgatgca cttcacgatc agcatccgca gccccgaaga
     1021 gatgggcgag gacacggcgg cgcgcgtcgc ggagggcttt cgtaccatcg aggtcaagct
     1081 cggccgattc gaaggagaac tcgacctgga taccgagatc gcacggatcg cggcgatccg
     1141 cgaggcagcg ggtcccgacg tcgtcatcat cgccgacccc aacatcgggt ggacgacgca
     1201 ggaggcgatc gacgtgttgc cgagcatgga gcagttcaac gtgtacgtcg agcagcccgt
     1261 gaagggcatc gaggcgctcg gacaggtccg cgccgcgatc tcgtcacccg tcatcgccga
     1321 cgagagcgcg gcgaacgctc gggtgttggc cgagatcatc cgtcgcgaag cggccgacct
     1381 gatcaacctc aagatcctgc gcaccggcgg gctgtacgaa gcaagcaaga tgctcgacat
     1441 ctgcgagtcg ttcggcatcg gctaccgcca cgacaacgtg atccagtcgc gccttgcctc
     1501 gacgatgatg attcacttct ccaccaccta caaccgcggc ctgcccgacg gcggcggcac
     1561 acagttcatc cgaaccgtgc aggacgtggt caaccccgac cagggggtgt acctcgacgg
     1621 tggggtcgcg aagctacgcg accccgacgc gccagggctg ggtgccacgc cgcgcgagga
     1681 gttgctccgc gacccgatcg tgatcgccgg ctgaccagcc agcggactcc tcagcgcgtc
     1741 acgagcggac gggccgcgcc tggggcgtga tgaccatcgg ggccatcgag gtgatgtcga
     1801 cgtcgagcac ccaggtaccg ccggcggcga ccgcggccga gaatgctgcg gcgaactcgg
     1861 ccggcgtcgc gacgtgtgcg ctgcgcaccc cgaacgcggt gccgagtgcg acgaagtcgg
     1921 gtgtcgccag ctcgactcct gtccgccggc ctgcgacggc catgtcctgg atgaaccgca
     1981 gcatcccgta gccgcgatcg ttgaacacac acaggatgag cgccaggtcg tactgcgccg
     2041 cggtcgccag ttcaccgacg ctgagcatca gcccgccgtc gccttggacg acgaccgtcg
     2101 tacgcccggt tgtggccgcg cccacggcca acggcaggcc cggtccgatc gcctgcgacg
     2161 tcggccgcat cgacgaccga gtctgggtga ccggcacgat ccggttcgcc cagaggtagg
     2221 ccgagatggt cgcgtccttg acgacgatcg cgtcgtccgg caaggtcgag cagatcgcgt
     2281 cgacgatcgc gtccatgtcc gggccgatgt cgacacggag ttgggcgacg acttcgtcac
     2341 gggttcgctg gcatgacgcg acccagtccg ggtgtgtcgc tctgccgtcg ccgaccgcgg
     2401 cgagcaactg gtcgaggccc agtcgggcat cgccgacgag cgccacgtct gcgggatgga
     2461 cgcggccgat caccgccgga tcggcatcca ggtggatcaa cctgccgggg aaccgcagcc
     2521 acttgagcac gttcgaatac tgctgaaacc tggtgccgac cgcgagcacc acatcggctt
     2581 gggcgaagac ggagtccatc gcggccatgt ccatctgggc accgagcgcg agctcgtgcc
     2641 gctcggagat cgcgccgcgt ccctcgatcg tggtcacgac cggtgcgtcg agaccttcgg
     2701 ccaacgccgt cagcgcggca ccg
//
LOCUS       DAORNF010000462         2723 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_28214_length_2723_cov_1.434783, whole genome shotgun sequence.
ACCESSION   DAORNF010000462 DAORNF010000000
VERSION     DAORNF010000462.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2723
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28214_length_2723_cov_1.434783"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(146..1129)
                     /locus_tag="PLV68_15955"
     CDS             complement(146..1129)
                     /locus_tag="PLV68_15955"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate ABC transporter substrate-binding
                     protein"
                     /protein_id="HRE02795.1"
                     /translation="MVTLTACSGDARLPDAIEVSVPTTTAPQTPAAPTCASTDQFFGS
                     AAVASYPALDPLPTADNLPPGSAMAAIRARGRLIIGVSADTLQFGARNPVTGAIEGFD
                     IDILKEVGKAIFGTDDPPIEYRVITYAQRLPVLENHEVDLVAHTMTINCNRWLRIAFS
                     TEYFPAGQKVLVRLQDDGQSNLVPAYDSIEAINDFRGRICVPGGSTNLEEIQKPEYSG
                     LQIVERVDITDCLVAFQQGQVNAITGDDTVLVGFAAQDPYAVVVGDAFTSEPYGVGIA
                     KDQPELVQFVNGVLAKIRTDGTWEDIAARWIDAANIPPVPQADESRPLSGG"
     assembly_gap    1299..1328
                     /estimated_length=30
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1329..1584)
                     /locus_tag="PLV68_15960"
     CDS             complement(<1329..1584)
                     /locus_tag="PLV68_15960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02796.1"
                     /translation="MTLINRGNGQANEAAYNQSAQVTLTILDRRRPITAVDWEEYGTL
                     HAGIRAADDSGDWDGAVVLLNGESAAVFADVTADIEARVAS"
     assembly_gap    1622..1795
                     /estimated_length=174
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1796..2367)
                     /locus_tag="PLV68_15965"
     CDS             complement(<1796..2367)
                     /locus_tag="PLV68_15965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02797.1"
                     /translation="MNAPVQTTRPTSLSASTPARLRALSAISAVLAVVLGVFGWVAIS
                     HRADALDRASDAAQRLIAVQEIRSAVIEADSIATTSFLVGGLEPAEQRARYEERLATA
                     ANQMAEQQLDQVSATLTTVAGLVEQARANNRQGFPVGAAYQRQASSVVRDDLLPALDV
                     IERDTRTMVNDAIASAHRIGWVVPFSGVVM"
     gene            2438..>2723
                     /locus_tag="PLV68_15970"
     CDS             2438..>2723
                     /locus_tag="PLV68_15970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02798.1"
                     /translation="MPESTRELEAATWAVAEGRATHAELALFQAEPQASQRTLLRLIV
                     DTEDTLASVRTLPTGDRDQVVTDFEEELARLEAILASLVPAVHTDAPVVGE"
BASE COUNT          386 a          832 c          880 g          421 t
ORIGIN      
        1 gaagttcaac cagggcttcg cgaccaccga gtacctcggc gcggccctgc ttgagcgcga
       61 ggaactgggg cgtcttgtcg agccgttgct cgcgcgccgg aaacacgatg gcgctgatcg
      121 acggtgccgc agtgatcacc gctgctcagc ctcccgacaa cgggcgcgac tcgtcggcct
      181 gcggaaccgg ggggatgttg gctgcatcga tccagcgcgc cgcgatgtcc tcccacgtgc
      241 cgtcggtgcg gatcttggcc agcaccccgt tgacgaactg caccaactcg ggctgatcct
      301 tggcgatgcc gacgccgtac ggctcggacg tgaacgcatc gccgaccacg accgcgtacg
      361 ggtcctgcgc ggcgaagccg accagcacgg tgtcgtcacc ggtgatcgcg ttgacttggc
      421 cctgctggaa cgcgaccagg cagtcggtga tgtcgacccg ctcgacgatc tgcagcccgc
      481 tgtactccgg cttctggatc tcttccaggt tggtgcttcc accgggcacg cagatgcgcc
      541 ccctgaagtc gttgatcgcc tcgatcgagt cgtaggccgg gacgaggttg ctctggccgt
      601 cgtcttgtag gcgcaccagc accttctggc ccgcggggaa gtactcggtg ctgaacgcga
      661 tgcgcagcca gcggttgcag ttgatcgtca tcgtgtgcgc gaccagatcg acctcgtggt
      721 tctccaacac cggcaggcgc tgggcgtagg tgatgacccg gtactcgatc gggggatcgt
      781 cggtgccgaa gatcgccttg ccgacctctt tgagaatgtc gatgtcgaag ccttcgatcg
      841 cgcccgtcac cgggttgcgg gcgccgaact gcaacgtgtc cgccgagacg ccgatgatca
      901 gccgcccgcg tgctcggatg gcagccatgg cggagccggg cggaaggttg tcggcggtcg
      961 gcagagggtc gagtgccggg tacgaggcga ccgccgccga accgaagaac tggtcggtgc
     1021 tggcgcaggt cggcgccgcc ggcgtttggg gcgcggtggt ggtcggtacc gagacctcga
     1081 tcgcgtcggg caagcgggcg tcacccgaac aggccgtgag cgtcaccatc gaacccgcca
     1141 cgatggcggc cccgagcgag ccgaaccgac cgcgacgggc gaagacgggt gcgtgccgcc
     1201 gcatcagctg tactccttga ggcgctggcc gtaaccgacc acgaacagtc cggcggcgac
     1261 gagggcgccg atgatgatca tccaccggct ggtcggcann nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnngg ctggcgaccc gcgcttcgat gtcggcggtc acgtcggcga acacagcggc
     1381 cgactcgccg ttcagcagca ccacggcccc gtcccagtca ccgctgtcgt cggcggcgcg
     1441 gatgccggcg tgcagagtgc cgtactcctc ccagtcgacc gcggtgatgg ggcggcgccg
     1501 atcgaggatc gtcagcgtga cctgggccga ctggttgtag gcggcctcgt tggcctgacc
     1561 gttgccgcgg ttgatcaacg tcaacgccag ccgatctgcc agcggccgaa attcctccat
     1621 cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnatcat
     1801 gacgacccca gagaagggga ccacccagcc gatgcggtgg gccgacgcga tcgcgtcgtt
     1861 gaccatggtg cgggtatcgc gttcgatcac gtcgagtgct ggtaggagat cgtcgcgaac
     1921 caccgacgac gcctggcgct gataggcggc cccgaccggg aagccctgac ggttgttggc
     1981 acgggcctgt tcgaccagcc cggccacggt ggtgagcgtg gcgctgacct gatcgagctg
     2041 ctgttcggcc atctggttgg ccgcggttgc caaccgctcc tcgtaccgcg ctcgttgctc
     2101 ggccggctcg aggccgccga ccaggaagct ggtggtggcg atggagtcgg cttcgatgac
     2161 agcgctgcgg atctcctgca ccgcgatcag ccgttgcgcg gcgtcgctgg cccggtcgag
     2221 cgcgtcggcc cggtggctga tcgcgaccca cccgaacaca ccgagcacca cggccaacac
     2281 cgcgctgatc gcgctcaacg cgcgcagccg ggcgggcgtc gacgcgctga ggctggtggg
     2341 cctcgtggtt tgcactggtg cgttcactgt cgtgatggtg gcttcccttc gctggccgcc
     2401 gatcttacgt ggggagtcga tatggtggag cccacgcatg cctgaaagta ctcgggagct
     2461 cgaggcggcg acatgggcgg tggcggaagg tcgcgccacc cacgccgaac tcgctctgtt
     2521 ccaagccgaa ccgcaagcgt cgcagcgcac gttgctgcga ctgatcgtcg acaccgagga
     2581 cacgctcgcg tccgtacgca cattgcccac cggtgatcgc gatcaagtgg tcaccgattt
     2641 cgaggaggaa ctggctcggc tggaggcgat cttggcctcg ctggtgccgg ccgtgcacac
     2701 cgacgccccc gtcgtgggcg agg
//
LOCUS       DAORNF010000463         2723 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_28215_length_2723_cov_1.429910, whole genome shotgun sequence.
ACCESSION   DAORNF010000463 DAORNF010000000
VERSION     DAORNF010000463.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2723
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28215_length_2723_cov_1.429910"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1136
                     /locus_tag="PLV68_15975"
     CDS             <1..1136
                     /locus_tag="PLV68_15975"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HRE02799.1"
                     /translation="DLARLGAIASTTGVRVLLADSTNSEEHGHAPSESSVGAVLRAVF
                     AEHRGRRIITASFASHLHRIQQIADAAIAEGRIVATLGLSIKKNVRMGRDLGILRIPD
                     ASLIDIEEADKYPPERLCIVSTGSQGEPMSALGLLARDENRWVKVGDNDTVILSSHAI
                     PGNEGNVNRVIDGLLRRGVEVVHSGIVDVHATGHAQADELKTYLSIVRPEYYVPIHGE
                     YRHLVANAKLGRLMGVAADNVIVCEDGDVLDLTDDGLAKAGRVPAGYLYVDGIVGDVG
                     QGVLRDRREEGVVVVVTTVDIGSGKVLTGPEIITRGWVYAPEAEDLLDEACDRVAQAV
                     EAALAKGERNVDALEREVRRAAGRFVSERTKRRPMIVPVVMEA"
     gene            1137..1781
                     /locus_tag="PLV68_15980"
     CDS             1137..1781
                     /locus_tag="PLV68_15980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02800.1"
                     /translation="MARRARRSCLVAVAGLAALTLGTACSSDGNGRDGEGGAVQGPTT
                     TARTLTDAESPLRVGQIGAAVAAVEAAEGGAARYFEINASPTVVNLFVAREADANSTT
                     SDMAVVAYVYAAGALQAPSEPSPAAGPTFAYSSVSIDTARVLSPTLAQLPTSTPRLFS
                     IVGVADADAAQYQIVVESSQGTELTVFVRGDGEIIGTDLQMELPESVGSILTGG"
     assembly_gap    2013..2117
                     /estimated_length=105
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2376..2385
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2386..>2723
                     /locus_tag="PLV68_15985"
     CDS             <2386..>2723
                     /locus_tag="PLV68_15985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02801.1"
                     /translation="RALLGDIGGFVLLLAIGVGGLLLVTNLTIRSLLARTGQGVGQLA
                     RPLGQAARRKIGDLSTLRSDRDDTLALDPAPQVYDAEAEEDFSAPKPARSRKPRALPT
                     PDPLLAEHEV"
BASE COUNT          380 a          883 c          939 g          406 t
ORIGIN      
        1 ccgacctggc tcgtctcggc gcgatcgcat cgaccaccgg cgtgcgggtg ctgctcgccg
       61 atagcaccaa cagcgaagag cacggacacg cgcccagcga gtcgagcgtc ggtgcggtgt
      121 tgcgggcggt gttcgcggag caccgcgggc ggcggatcat cacggccagc ttcgccagcc
      181 acctgcaccg catccagcag atcgccgacg cggccatcgc cgagggccgc atcgtggcca
      241 cgctggggct gagcatcaag aagaacgtgc ggatgggtcg tgacctcggc attctgcgca
      301 ttcccgacgc gtcgctgatc gacatcgagg aggccgacaa gtacccgccc gagcggttgt
      361 gcatcgtgtc gaccgggtcg cagggcgagc ccatgtcggc actcggcttg ctcgcccgtg
      421 acgagaaccg gtgggtcaag gtcggtgaca acgacacggt catcctgtcc agccacgcca
      481 tccctggcaa cgagggcaac gtcaaccgcg tcatcgacgg gctgctgcgc cgcggtgtcg
      541 aggtggtgca cagcggcatt gtcgacgtgc acgccaccgg gcacgcccag gccgacgaac
      601 tgaagaccta cctgtcgatc gtgcgtcccg agtactacgt gccgatccac ggcgagtacc
      661 gccacctggt tgccaacgcc aagctcggtc gcctgatggg cgtcgccgcc gacaacgtga
      721 tcgtctgcga ggacggcgac gtactcgacc tgacagacga cggtctcgcg aaggcggggc
      781 gcgtgccagc cggatacctg tacgtcgacg gcatcgtcgg cgacgtcggc cagggcgtgc
      841 tgcgcgaccg gcgggaagag ggcgtggtcg tcgtcgtcac cacggtcgac atcggttcgg
      901 gcaaggtgct gacgggcccg gagatcatca cccgtggctg ggtgtacgcg cccgaggccg
      961 aggacctgct cgacgaagcc tgcgaccgcg tcgcccaggc ggtcgaggcc gcgttggcca
     1021 agggcgaacg caacgtcgat gcgttggaac gtgaggtgcg ccgggccgcg ggacgattcg
     1081 tcagcgagcg caccaagcgc cgcccgatga tcgtccccgt cgtgatggag gcctgagtgg
     1141 cgcgtcgagc gcggcgttca tgtctcgtcg ctgttgctgg tctcgctgcg ctgacgctcg
     1201 gaacggcctg ttcgtcggac ggcaacggcc gcgacggtga gggcggtgcc gtccagggtc
     1261 cgaccacgac ggccaggacg ctgaccgatg ccgagtcgcc gttgcgcgtc ggtcagatcg
     1321 gtgccgcggt cgccgcggtc gaagcggccg agggcggcgc ggcgcggtac ttcgagatca
     1381 acgcctcgcc cacggtggtc aacctgttcg tcgcccgcga ggccgacgcg aacagcacga
     1441 cgagcgacat ggctgtcgtc gcctacgtct atgcggctgg cgcgctgcag gcaccgagcg
     1501 agccatcgcc ggccgcgggt ccgacctttg cgtactcgtc ggtgtcgatc gacacagccc
     1561 gcgtgttgtc ccccacgttg gcccaactgc cgacctcgac gccgcggttg ttctcgatcg
     1621 tcggggtggc cgatgctgac gcagctcagt accagatcgt cgtcgaatcc agccaaggca
     1681 ccgaactgac ggtgttcgtg cgcggcgacg gggagatcat cggtaccgat ctgcagatgg
     1741 agttgcccga gtcggtcggg tcgatcctca ccggcggctg acaccgcccg acggcgattg
     1801 ccggacgggg cgcgcgcccc cttggtaccg ttgcagacct atgaccacct ggggacgcga
     1861 gtcccgcaag tcgcaaccag ctcgatcctc ggcacgctcg tcgtccgccc gcgcgggcgc
     1921 agcgcgtcag acctccgggc gcagcggggg atctcgcggt gcctccagcc gcgacgagtc
     1981 gggttcgctc gaccggcgcg ccagtgctcg tgnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnncgg gcgcttcgtc aacacgttgc tggggtggtt caccggtctc
     2161 ggccggttcg tggtaccggt ggcattggtg gccgtcggtg tggcactggt gcaccgtggc
     2221 cgcagcgcca gccgcgtgcg cttggcggtc gggtggggtg ccatggccgt cgccgcgctc
     2281 ggcctgttgc acctggcgcg cgaagacggt ggcatcaccg tcgagaccga ccggctcgag
     2341 cgtgcgggcg gctggatcgg ctcgctcgtc gccacnnnnn nnnnngcggg cgctgttggg
     2401 cgacatcggc ggcttcgtgt tgctgctggc gatcggcgtc ggtggtctgc tgctggtgac
     2461 caacctgacc attcgctcgc tgctcgcccg caccggccaa ggcgtcgggc aactagcccg
     2521 gccgttgggg caggcggcgc ggcgcaagat cggcgatctg tccacgctgc gcagcgaccg
     2581 tgacgacacc ttggcgctcg acccagcgcc gcaggtctac gacgccgagg ccgaggaaga
     2641 cttctccgcg cccaagcctg cgcgcagccg caagccgagg gcgttgccga cccctgatcc
     2701 gctgttggcc gaacacgagg tgg
//
LOCUS       DAORNF010000464         2722 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_28228_length_2722_cov_1.428196, whole genome shotgun sequence.
ACCESSION   DAORNF010000464 DAORNF010000000
VERSION     DAORNF010000464.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2722
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28228_length_2722_cov_1.428196"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    301..383
                     /estimated_length=83
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<384..657)
                     /locus_tag="PLV68_15990"
     CDS             complement(<384..657)
                     /locus_tag="PLV68_15990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02802.1"
                     /translation="MVAALDSTRLVDRADVGEFTVARRDGFEGSGGGVCLTTAAGETG
                     CANFDAVGSFASVALDGQWYIVAVVPNTEPTTTFRTEPDLRFDAADG"
     assembly_gap    688..737
                     /estimated_length=50
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<738..>1037)
                     /locus_tag="PLV68_15995"
     CDS             complement(<738..>1037)
                     /locus_tag="PLV68_15995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02803.1"
                     /translation="LAASDVEAAVAEAIDDLLPGFVPSDGSWRNVGTGYAIRYQSGRG
                     QVFLTANPLSGPIDLETINPVQSDEAGDFVLTHTFDNEPWDLWWVDHGFLIRLIG"
     assembly_gap    1038..1062
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1063..1538)
                     /locus_tag="PLV68_16000"
     CDS             complement(<1063..1538)
                     /locus_tag="PLV68_16000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02804.1"
                     /translation="MRTTRIIALTVVALALVGCGRESGASDAGDGADRGGADASAPVT
                     AAVDSEPTARSTGTIAEQDDLGEVVGVVLAAEGFVPVFVDPARLAQSPFTDAQRADVY
                     RGADGASLVVLTSTYPDGTGITDAELSHLDPGDGRNCWVSMAWPDAVRSLRLTSTD"
     gene            complement(1548..1994)
                     /gene="nusB"
                     /locus_tag="PLV68_16005"
     CDS             complement(1548..1994)
                     /gene="nusB"
                     /locus_tag="PLV68_16005"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HRE02805.1"
                     /translation="MARRPRDEDERSDARERALGYLYEAETKAIAPTDVLAAQVITPD
                     ALTTMLVTGVERNGQRIDDLVAAHAKGWTLARMPSIDRNVLRLGTFELLERPDVDVPV
                     VIDEAVALAVRFSTDDSGRFVNGVLAAIADTVRGGSARHPATPTEP"
     gene            complement(1997..2560)
                     /gene="efp"
                     /locus_tag="PLV68_16010"
     CDS             complement(1997..2560)
                     /gene="efp"
                     /locus_tag="PLV68_16010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HRE02806.1"
                     /translation="MPAITTNDLKNGITLELDSGLFQVVEFQHVKPGKGGAFVRTKLR
                     NVRTGATLERTFNAGIRVEQAILDRYDMQFLYRDGDDYVFMNTESYDQSNVAPVTLGE
                     AADYMIEGMMAQIAYFNGEIVTVEIPASVELTISETEPGLQGDRSTGGRKPATLETGK
                     QIQVPLFVNIGDKVKVDTRTGEYMTRA"
     gene            complement(2533..>2722)
                     /locus_tag="PLV68_16015"
     CDS             complement(2533..>2722)
                     /locus_tag="PLV68_16015"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HRE02807.1"
                     /translation="WLTQGFDDTLAAMDVVTVEPGVYRDDFGGVRIEDLVVVATDGCR
                     SLTQTPKDSPCLPSRPTT"
BASE COUNT          434 a          890 c          826 g          414 t
ORIGIN      
        1 tgcagcttcc gcggagtacc aatccaccgg aaaccggcca acccccactc cgcggacgcc
       61 cgattcgcca cgccggccaa tccagcgtcc gcggagtacc gatgcaccgg tttccgggca
      121 atccaggctc cgcggacgcc gaaatcgcca cgccggccaa tccagcttcc gcggacgggc
      181 aactcgccaa accggagaat ctggcttccg cggagtaccg atgcaccggt ttccggccac
      241 tccacgatcc gcggacgccg aattcgccgg gctactgctg gggccgggtc caggtggtcg
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnngcccgtc cgcagcgtcg aaacgcaggt cgggttcggt
      421 gcggaacgtc gtggtcggct cggtgttggg cacgacggcg acgatgtacc actggccgtc
      481 cagcgcgacg ctggcgaaac tacccaccgc atcgaagttg gcgcacccag tctctcccgc
      541 agcagtggtc agacagactc cgccacccga gccctcgaac ccgtcccgac gggcaaccgt
      601 gaactcaccc acgtcagccc gatcgaccaa ccgcgtcgaa tcgagcgcag ccaccatcgc
      661 cgcacgagcg tcggcaatcg ggtccgannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnccc cgatcagccg gatcaagaac ccgtgatcaa cccaccacag
      781 atcccacggc tcgttgtcga acgtgtgcgt cagcacgaag tccccggctt cgtcggactg
      841 caccgggttg atcgtctcca agtcgatcgg tccgctcagt gggttggccg tcaggaacac
      901 ctggccacgg cccgactggt agcggatcgc atagcccgtg ccgacgttgc gccacgatcc
      961 atccgacggc acgaacccgg gcagcagatc atcgatcgcc tcggccacgg cagcctcgac
     1021 atcggacgcg gccagcannn nnnnnnnnnn nnnnnnnnnn nntcgtcggt gctcgtgagg
     1081 cgcagcgagc ggaccgcatc tggccaggcc atgctcaccc agcagttccg cccgtcaccg
     1141 ggatcgagat ggctcaactc ggcgtccgtg ataccggtcc cgtcgggata cgtcgatgtg
     1201 agcacgacca gacttgcacc atccgctccg cggtagacgt cggcgcgctg tgcgtcggtg
     1261 aacggcgact gggccagccg cgccgggtcg acgaagaccg gcacgaaccc ttccgccgcc
     1321 agcaccaccc cgacaacctc accgaggtca tcctgttccg cgatcgtacc ggtgctcctc
     1381 gcggtcggct cgctgtcgac cgctgccgtc accggcgccg acgcatcggc accaccgcga
     1441 tctgcaccgt caccggcatc gctcgcgccc gactccctgc cacaccccac caacgccagc
     1501 gcaacaaccg tcaacgcaat gatccgcgtc gtgcgcaccg ggcgatgtta gggctcggtg
     1561 ggggtggcgg ggtggcgggc ggagccgccg cgcaccgtat cggcgatcgc ggccaggacg
     1621 ccgttgacga agcggccgga gtcgtcggtg gagaaacgca cggccaaggc gacggcttcg
     1681 tcgatcacga ccgggacgtc gacatcgggg cgttcgagca gttcgaacgt gcccagacgc
     1741 aacacgttgc gatcgatcga gggcatccgc gccaacgtcc aacccttggc gtgagcggcg
     1801 acgaggtcat cgatgcgttg gccgttgcgc tcgacgccgg tgaccaacat cgtggtcaac
     1861 gcatcgggcg tgatcacctg cgcggcgagc acgtcggtcg gagcgatcgc cttggtctcg
     1921 gcctcgtata ggtagccgag ggcgcgttca cgggcgtcgg agcgttcgtc ctcgtcgcgg
     1981 ggacggcggg ccacgatcag gcccgggtca tgtactcgcc ggtgcgagtg tcgactttga
     2041 ccttgtcgcc gatgttgacg aacagtggta cttggatctg cttgcccgtc tccaaggtgg
     2101 ccggcttgcg cccaccggtg gagcggtcgc cctgcaggcc gggctcggtc tcggagatgg
     2161 tgagctcgac cgaagccggg atctcgacgg tgacgatctc gccgttgaag taggcgatct
     2221 gggccatcat gccctcgatc atgtagtcgg ccgcctcgcc caaggtgacc ggcgcgacgt
     2281 tgctctggtc gtacgactcg gtgttcatga acacgtagtc gtcgccgtcg cggtacaaga
     2341 actgcatgtc gtagcggtcg aggatggcct gctcgacccg gatgccggcg ttgaacgtgc
     2401 gctcgagcgt ggccccggtg cgcacgttgc gcagcttggt gcgcacgaac gcgccaccct
     2461 tgcccggctt gacgtgttgg aactcgacca cttggaacag cccgctgtcg agctcgaggg
     2521 tgattccgtt cttcaggtcg ttggtcgtga tggcaggcat ggtgaatcct tgggcgtctg
     2581 ggtgagggac cggcaaccgt cggtcgcgac gaccaccaga tcctcgatcc gaacgccgcc
     2641 gaagtcatca cgatagacgc caggttccac ggttacgacg tccatcgccg cgagtgtgtc
     2701 gtcgaagccc tgcgtcagcc ac
//
LOCUS       DAORNF010000465         2721 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_28250_length_2721_cov_1.429107, whole genome shotgun sequence.
ACCESSION   DAORNF010000465 DAORNF010000000
VERSION     DAORNF010000465.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2721
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28250_length_2721_cov_1.429107"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(238..>838)
                     /locus_tag="PLV68_16020"
     CDS             complement(238..>838)
                     /locus_tag="PLV68_16020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02808.1"
                     /translation="LKLGKQRSDLLNHLPQLTMCDLLAAKGWGRPSLGLVVPREVKGF
                     RREDHTGDAGHVAKMNRAAQGSLLAQDAPTLEFSPHAFKLHYLCQSDSCPSHTQTIVD
                     WEISEAWRTWRRDYPNDYLDRIEDKWMGLVDAGRQPAFYVGNQQQAPQGFLVLGIARD
                     ITRREPTPVDPGRQDASPRSTDGMNKDQPRSTEGRLFDL"
     assembly_gap    839..953
                     /estimated_length=115
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1227..1314
                     /estimated_length=88
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1401..>1615)
                     /locus_tag="PLV68_16025"
     CDS             complement(1401..>1615)
                     /locus_tag="PLV68_16025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02809.1"
                     /translation="KYPDRSLSSLYEPDAMPKDLVAAHDQVDEVIDGLFGAVQDISED
                     DRQRLLFRMYGEMAGKKPSTAGARRR"
     assembly_gap    1616..1767
                     /estimated_length=152
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1768..>2186)
                     /locus_tag="PLV68_16030"
     CDS             complement(<1768..>2186)
                     /locus_tag="PLV68_16030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02810.1"
                     /translation="GAAWPAEVRKDAIASKYLRRFVGARELIHDIERWCLWLVDATKA
                     ELRDSAELKSRIAAVKKMRSESTKAATRAKAATPHLFDERRQPTTSYLAIPRHVGEQR
                     RYFTALRLPPDVICGDANFMAPDAEGFALGQAVDAVS"
     assembly_gap    2187..2196
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2197..>2721)
                     /locus_tag="PLV68_16035"
     CDS             complement(<2197..>2721)
                     /locus_tag="PLV68_16035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02811.1"
                     /translation="FIGQYTKTPEQKADAKRIWGDRYNGYLDYVTCWYARTLDYYGKH
                     AGRWAFVSTNSICQGEATEYLWRPILEAGWRCRFAHRSFRWVTEAADGAAVHVSILGF
                     DRVTSPEPVLWTYSVGGTGSGTPEVAKQINPYLVADADIVLVTSQTRPMSAALPPVDY
                     GNKPTDGGNLFVHP"
BASE COUNT          389 a          735 c          785 g          447 t
ORIGIN      
        1 tctcagctct ggttgatgtc cgcgagaacg cgatcagtcg caagtcgggg ctgtcgaagc
       61 gggcgctcgc cgcacgctgc gaagcgagcg ggatccgcta cgtgcatgag ccgacgcttg
      121 gcaatccacg tgcgaaccgg gacggctttc gtgaagggag gcccgagagc atcgccgcgt
      181 tcgaggcgca tgtggaggag gtgggtggcg acgcgctcga tcgcgttgct gagatgctca
      241 gagatcgaac agtcggcctt ctgtgcttcg aggctgatcc ttgttcatgc catcggtcga
      301 tcgtggcgaa gcatcttgtc ggccgggatc cactggtgtc ggttcgcgac gtgtgatgtc
      361 gcgtgcgatg ccgagtacca ggaagccttg tggggcttgc tgctggttgc cgacgtagaa
      421 ggctggttgg cggccggcgt cgaccagtcc catccacttg tcctcgatcc ggtcgaggta
      481 gtcgttgggg tagtcgcgtc gccacgtccg ccatgcttcc gagatctccc agtcgacgat
      541 tgtctgggtg tggctcgggc acgaatctga ctggcacagg tagtggagct tgaacgcgtg
      601 cggggagaac tcgagcgtgg gggcgtcctg tgcgagcagg ctgccctggg cggcacggtt
      661 catcttggcc acgtgcccag cgtcgccagt gtggtcctca cggcggaagc cctttacctc
      721 gcgcgggacg acgaggccaa gtgaaggccg accccaaccc ttggcggcta agaggtcgca
      781 catagtcaac tggggaaggt ggttgagcag gtcggaacgc tgtttcccca gtttgagann
      841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnccagaac
      961 cactgccatc gcaccgggaa cagcctgatc cactggggtg tctccgtgtc ggtgcggatg
     1021 ccggcgacgc agaccgtctc gccgtgcgtt cgtccgatct ccggcgacgc cttgacggtg
     1081 atcacgaccg tggcccgctc aacccccatg cgtggatggt atgacacccc gcccgtctgc
     1141 ctaccacgga tcggcgcccg ccgacgagcc tgagccgagt gtccagctcg gagccggatc
     1201 cgtcttgtcc cccgccaacg ggcctgnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnatgaca
     1321 caacggcgtg ccgggagcgc gactcgacga gcgagggcga agcacgcgga tatacgcctg
     1381 acgcgcgggt cgccaacgac ttagcgacgc cgggcccctg cggtggacgg cttcttgccg
     1441 gccatctcgc cgtacatcct aaacaggagc cgttggcggt cgtcctcgga gatgtcctga
     1501 actgcaccga agaggccgtc gatgacctca tccacctggt cgtgtgccgc cacgagatcc
     1561 ttgggcatcg cgtcgggttc gtacagcgat gagagtgagc ggtcggggta cttgcnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnggc tgacggcatc gaccgcctgg cccagggcga
     1801 agccctcggc gtcgggtgcc atgaagttgg catcaccaca gatgacgtcg ggtggcaggc
     1861 gcaacgcggt gaagtagcga cgctgctcac cgacgtgtcg aggaatcgcg agatagctcg
     1921 tcgtcggttg ccgtcgttcg tcaaacagat gcggagttgc agctttggct cgtgttgccg
     1981 ccttggtgct ctcggaacgc atcttcttga cagcggcgat acgcgacttc agctccgcgc
     2041 tgtcgcgtag ttcagccttg gtcgcgtcca ccagccacag gcaccatcgc tcgatgtcgt
     2101 ggatgagttc tcgtgccccg acgaatcggc gcaggtactt cgatgcaatg gcgtccttcc
     2161 gtacctccgc aggccaggcg gcgccgnnnn nnnnnncagg atgaacaaac aggttgcccc
     2221 cgtcagttgg cttattgccg tagtccacgg gtggcaacgc ggcgctcatc gggcgcgtct
     2281 ggcttgtcac gagcacgatg tctgcgtcag ccaccaggta ggggttgatc tgcttcgcga
     2341 cctcaggcgt gccgctgccc gtcccgccaa cggagtacgt ccacagcacc ggctcagggc
     2401 tcgtcacccg gtcaaaacca aggatcgata cgtgcactgc tgcgccgtcg gcggcctccg
     2461 tcacccaacg aaacgagcgg tgcgcgaagc ggcaacgcca gcccgcctcc aagatcggtc
     2521 gccacaggta ctcggtcgcc tcgccctggc agatcgagtt cgtggacaca aacgcccaac
     2581 gccctgcgtg cttgccgtag tagtcgagtg tgcgggcgta ccagcaggtc acgtagtcga
     2641 ggtagccgtt gtagcggtca ccccaaatgc gcttcgcgtc ggccttttgt tccggtgtct
     2701 tggtgtactg cccgatgaac g
//
LOCUS       DAORNF010000466         2711 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_28390_length_2711_cov_2.220633, whole genome shotgun sequence.
ACCESSION   DAORNF010000466 DAORNF010000000
VERSION     DAORNF010000466.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28390_length_2711_cov_2.220633"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..551)
                     /locus_tag="PLV68_16040"
     CDS             complement(<1..551)
                     /locus_tag="PLV68_16040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02812.1"
                     /translation="MQATHPSRRRSAAALLAGLLVATAAGCSGDDAASETTRRSRRTD
                     PDVTVERDRDTTTTSTIADTIASTTTEPADVVQPGWLVVVYEAADNNLEAAILTDVAE
                     MGAVDNPDIDIVVLFDRSSESDRSGGFTDADTAPGIPEQVGSTQLLHAGQGGVSLLGT
                     IEDVDSGDPAVLAEFVEAAVTNYP"
     gene            complement(674..2311)
                     /locus_tag="PLV68_16045"
     CDS             complement(674..2311)
                     /locus_tag="PLV68_16045"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HRE02813.1"
                     /translation="MGSIVVSDLEYAPPGGDSLFFDVSFTVGPGEKAALVGVNGSGKS
                     TILRILSGDLEPDAGEVAILGSTLAMTQDVGMSRPTDTLRELLLTVAPPALRTAGSAL
                     VAAERALANGDDDGMGYAAALTEWGDLGGYELEQRWEQAVRRSVKSEVDDVSTRLVGE
                     LSGGERKRLVLDLLLTSGAEVLLLDEPDNYLDIPTRLWLEEQIRASKSTVLMVSHDRV
                     LLQRVTTRIIALEGSGSWTHGGSFATFPEARAHRQELLGDALKRWNDEERRLFQHMKI
                     MKQRAAQNFKNATKANAAETRWEKFVAAGPPPPPVPDQQIVVRLRGADSARRVAVLDG
                     VSIGDLFLPFSTEVYAGERLGLIGPNGTGKTHLLSALAGTLSPDHGTISFGPRTSCGL
                     FTQVNDRPDFRGRTCLEIVTARVHEEEKGMRTLARYGLAGNARQEFQTLSGGQKARLE
                     ILCLEVEGHNVLLLDEPTDNLDVESSEALERALDGFQGTVIAVSHDRTFLAQLDRFVL
                     LNDDLDVFALPDYDTAMAALNAPAAVHTLRLAQRLTA"
     gene            2513..>2711
                     /locus_tag="PLV68_16050"
     CDS             2513..>2711
                     /locus_tag="PLV68_16050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02814.1"
                     /translation="MLAIDTASAVETVLPEDDRRGGRIVPLSAPVRLLDTRPGHPTVD
                     GQHAGGDVVGAGVTIEVPILGR"
BASE COUNT          402 a          918 c          945 g          446 t
ORIGIN      
        1 ggatagttgg tcacggccgc ttcgacgaac tcggccaaca ccgcggggtc accgctgtcg
       61 acatcctcga tggtgccgag caggctgacg ccgccctggc ccgcatggag caactgggtg
      121 gagccgacct gttcggggat cccgggggcc gtgtcggcat cggtgaaacc gccggagcgg
      181 tcggactcgc tcgagcgatc gaagagcacc acgatgtcga tgtcggggtt gtcgacggca
      241 cccatttcgg cgacgtcggt caggatcgcg gcctccaggt tgttgtcggc ggcctcgtag
      301 acgaccacca accatccggg ctgcacgaca tcggccggtt cggtcgtggt cgacgcgatc
      361 gtgtccgcga tggtggacgt ggtggtggtg tcacggtcgc gttcgacggt cacgtcgggg
      421 tcggtgcgtc gactccgacg ggtggtctcg ctcgccgcgt cgtcgccgga acacccggcc
      481 gcggtggcga ccaacaggcc ggccagcaat gctgccgcgc tgcgacgccg tgatgggtga
      541 gttgcctgca tggaattcct ccgccatgtg ccgcgaacga gacgtgcgag caatgtaccc
      601 ggccgctcag cggactcgca ggcaacgctc agccaacacg gccacaacaa ctcgacaaca
      661 acacggtggc cgcctatgcg gtcaggcgtt gggccaggcg gagggtgtgg accgcagcgg
      721 gggcgttgag ggcagccatc gccgtgtcgt agtcagggag ggcgaacacg tcgagatcgt
      781 cgttgagcag cacgaaccgg tcgagttggg ccaggaacgt gcggtcgtgg ctgaccgcga
      841 tgaccgtgcc ctggaagccg tcgagagcac gctccaacgc ctcggaggat tcgacgtcga
      901 ggttgtccgt gggctcgtcg agcagcagca cgttgtgccc ttcgacttcg agacagagga
      961 tctccaaccg cgccttctgc ccgccggaga gggtctggaa ctcctggcgc gcgttgcccg
     1021 ccaacccgta gcgggcgagg gtccgcatcc ccttctcctc ctcgtggacc cgcgccgtca
     1081 cgatctcgag gcacgttctg ccgcggaagt cggggcggtc gttgacctgg gtgaacagcc
     1141 cgcatgacgt gcgcggcccg aacgagatcg tgccgtgatc cgggctgagc gtgccggcca
     1201 acgcgctgag cagatgggtc ttgcccgtgc cgttggggcc gatcagaccc aagcgttcgc
     1261 ccgcgtacac ctcggtcgag aacggcagga acagatcgcc gatcgagaca ccgtcgagca
     1321 ccgcgactcg gcgcgcgctg tcggcgcccc gcaggcggac gacgatctgt tggtccggca
     1381 ccggcggtgg tggcggcccg gcggcgacga acttctccca gcgggtctcg gccgcgttgg
     1441 ctttggtggc gttcttgaag ttctgggcgg cgcgttgctt catgatcttc atgtgctgga
     1501 acagtcgccg ctcctcgtcg ttccaccgct tgagcgcgtc gcccagcaac tcttggcggt
     1561 gggcccgcgc ctcggggaag gtcgcgaacg agccaccgtg ggtccaactg cccgaccctt
     1621 cgagggcgat gatcctggtc gtgacgcgct ggagcagcac ccggtcgtgg ctgaccatca
     1681 acaccgtcga cttcgacgcc cggatctgct cttccaacca cagccgggtc gggatgtcga
     1741 ggtagttgtc gggttcgtcg agcaacagca cctccgcacc agaggtcagc aacaggtcga
     1801 gcacgagccg cttgcgttcc ccgccggaca gctccccgac gagccgggtg ctgacgtcgt
     1861 cgacctcgga cttgacgctg cgccgcacgg cctgttccca gcgctgctcc aactcatagc
     1921 cgccgaggtc gccccactcg gtcagcgcgg cggcgtaacc catgccgtcg tcgtcgccgt
     1981 tggccagtgc ccgctcggcc gccaccagcg cggagcccgc ggtgcgcaac gccggcgggg
     2041 ccaccgtcaa caacagctcg cgcagggtgt cggtcgggcg ggacatgccc acgtcctggg
     2101 tcatcgccag cgtcgaaccg aggatcgcga cctcgcccgc atcaggttcg aggtcccccg
     2161 acaggatccg caggatcgtg ctcttgcccg acccgttgac ccccaccagc gccgccttct
     2221 cccccggccc gaccgtgaag ctgacgtcga agaacagcga gtcgccaccc ggtggcgcgt
     2281 attccagatc tgacacgacg atgctgccca cggggccaga gtctggccga tcaccgctgc
     2341 cggggtggcg tgctttgcgc tggtttccca gtggttagcg cggtggttgc agcatcgccc
     2401 gatggcacgt cgacggaatg gctcgctctc gcccactgct ggctcacgcc ggggtccgac
     2461 ccgagtccgc tccggttcgt ggatcgggct gagcttggcg atggtggcgt cggtgctggc
     2521 gatcgatacg gcgtcggcgg tcgagacggt gctgcccgag gatgatcgac gcggcggacg
     2581 gatcgtgccc ctgtcagcgc cggtgcgcct gttggacacc cggcccggcc acccgaccgt
     2641 cgacggccag cacgccgggg gtgacgtggt cggcgccggg gtcacgatcg aggtgccgat
     2701 cctcggccgc c
//
LOCUS       DAORNF010000467         2710 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_28403_length_2710_cov_2.163842, whole genome shotgun sequence.
ACCESSION   DAORNF010000467 DAORNF010000000
VERSION     DAORNF010000467.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2710
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28403_length_2710_cov_2.163842"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>276)
                     /locus_tag="PLV68_16055"
     CDS             complement(<1..>276)
                     /locus_tag="PLV68_16055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HRE02815.1"
                     /translation="LSLDRPSGTLSGGESQRTKLIRHLGSALTDVTYVFDEPSIGLHP
                     HDIERMNELLLQLRDKGNTVLVVEHKPEVIAIADHVVDLGPGAGSRG"
     assembly_gap    277..319
                     /estimated_length=43
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<320..>714)
                     /locus_tag="PLV68_16060"
     CDS             complement(<320..>714)
                     /locus_tag="PLV68_16060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HRE02816.1"
                     /translation="GRIFKNCGYFDADKPISTFTKKELDALLYKEPTRIKVEGINVTY
                     EGLIPRIQKSFLSKDVEAMQPHIRAFVERAITFGVCPDCNGTRLNEAARSAKIAGISI
                     ADACAMQISDLAEWVRGLREPSVAPLLKGL"
     assembly_gap    715..724
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<725..1389)
                     /locus_tag="PLV68_16065"
     CDS             complement(<725..1389)
                     /locus_tag="PLV68_16065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HRE02817.1"
                     /translation="MSSTPQTSTTSERTGGDAADRHDRIRVHGARENNLKNMDVELPK
                     RRLTVFTGVSGSGKSSLVFGTIAAESQRLINETYSAFVQGFMPNLPRPDVDVLEGLTT
                     AIIVDQERMGANVRSTVGTATDANAMLRILFSRLGQPYVGPPNAFSFNLATVRGAGAI
                     TIEKSQGKTVEKREFARQGGMCPRCEGVGSVSDFDLTQLYDASKSISDGALTIPGYSM
                     DGW"
     gene            1550..1732
                     /gene="mftA"
                     /locus_tag="PLV68_16070"
     CDS             1550..1732
                     /gene="mftA"
                     /locus_tag="PLV68_16070"
                     /inference="COORDINATES: protein motif:HMM:TIGR03969.1"
                     /note="Mycofactocin is a small molecule electron carrier
                     derived from the final two amino acids, Val-Tyr, of MftA,
                     the mycofactocin precursor. It plays a role in redox
                     homeostasis and the metabolism of alcohols and aldehydes
                     in Actinobacteria, including Mycobacterium tuberculosis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin precursor MftA"
                     /protein_id="HRE02818.1"
                     /translation="MDAVLDAVIDDTIAVTAADTTAADTTAADTTAAATTAVESLVED
                     ELLIEEISIDGMCGVY"
     gene            1722..2006
                     /gene="mftB"
                     /locus_tag="PLV68_16075"
     CDS             1722..2006
                     /gene="mftB"
                     /locus_tag="PLV68_16075"
                     /inference="COORDINATES: protein motif:HMM:TIGR03967.1"
                     /note="MftB, a small protein, is a peptide chaperone that
                     assists the radical SAM enzyme MftC in performing two
                     modifications to the C-terminal Val-Tyr dipeptide of the
                     mycofactocin precursor peptide, MftA. MftB's role is
                     analogous to the role of PqqD in the biosynthesis of PQQ,
                     a cofactor that derives entirely from a Tyr and a Glu in
                     the precursor PqqA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin biosynthesis chaperone MftB"
                     /protein_id="HRE02819.1"
                     /translation="MSTDHALSSVLDAALTLSPQVALRPEPFGALAYHYGNRRLVFLR
                     HPDMVRVATALAEHPTLRATLEACAIAPPRWPSFERAIASLQTSEVICER"
     gene            1996..>2710
                     /gene="mftC"
                     /locus_tag="PLV68_16080"
     CDS             1996..>2710
                     /gene="mftC"
                     /locus_tag="PLV68_16080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443074.1"
                     /note="MftC is a radical SAM/SPASM enzyme that catalyzes
                     the first two steps in biosynthesis of the electron
                     carrier mycofactocin from the terminal Val-Tyr dipeptide
                     of the precursor peptide MftA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0031179 - peptide modification [Evidence
                     IEA];
                     GO_process: GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin radical SAM maturase"
                     /protein_id="HRE02820.1"
                     /translation="MSADTLVSQLKRGLDAPICLTWELTYACNLQCVHCLSSSGQRDP
                     RELTTAEAKRVLDELAELQVFYINIGGGEPMVRRDFFELIEYSVAKGIGVKFSTNGAF
                     IDTAKAARLAAMDYLDIQISLDGTDAATNDAVRGPGSYDMAIRAMQHLRDANFGPFKI
                     SVVVTRHNVDQLDEFKALADRYGAQLRITRLRPSGRGADTWHELHPTNAQQRQIYDWL
                     MANGEQVLTGDSFFHLNALG"
BASE COUNT          432 a          938 c          828 g          459 t
ORIGIN      
        1 caccgcgcga tccggccccg ggtccgagat cgaccacgtg gtcggcgatc gcgatcacct
       61 cgggtttgtg ctcgaccacc agcaccgtat tgcccttgtc acgcagctgc agcagcagtt
      121 cgttcatccg ctcgatgtcg tgcgggtgca ggccgatcga cggctcgtcg aacacgtagg
      181 tcacgtcggt gagggccgac ccgagatggc ggatcagctt ggtgcgctgc gactcgccgc
      241 cggagagcgt gcccgaggga cggtcgaggc tgaggtnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnna ggcccttcag cagcggcgcg accgacggct cgcgcagccc
      361 gcgcacccac tcggccaggt cgctgatctg catcgcgcac gcgtcggcga tgctgatgcc
      421 cgcgatcttg gccgaccgcg ccgcctcgtt gaggcgcgta ccgttgcagt cgggacagac
      481 gccgaacgtg atcgcccgct cgacgaaggc gcggatgtgc ggctgcatcg cttcgacgtc
      541 cttcgacagg aacgacttct ggatgcgcgg gatcagcccc tcataggtga cgttgatgcc
      601 ctcgaccttg atccgcgtcg gctccttgta gagcagcgcg tcgagttcct tcttggtgaa
      661 ggtcgagatc ggcttgtcgg cgtcgaagta tccgcagttc ttgaagatgc gcccnnnnnn
      721 nnnntaccaa ccgtccatgc tgtagccggg gatcgtcagc gccccatcgc tgatgctctt
      781 gctcgcgtcg tagagctggg tcaggtcgaa gtcgctgacc gagccgacac cttcacagcg
      841 cgggcacata ccgccctgac gggcgaactc gcgcttttcg accgtcttgc cctgactctt
      901 ctcgatcgtg atcgcgcccg cgccgcgcac cgtggcgagg ttgaacgaga acgcgttggg
      961 cggcccgacg tacggctggc cgagtcggct gaacaggatc cgcaacatcg cgttggcatc
     1021 ggtggccgtg ccgacggtgg agcgcacgtt ggcccccatc cgttcctggt cgacgatgat
     1081 cgccgtcgtc agtccttcga gcacgtcgac gtcggggcgg ggcaggttgg gcatgaagcc
     1141 ctgcacgaac gcgctgtagg tctcgttgat caaccgctgc gattcggccg cgatcgtgcc
     1201 gaacaccagc gagctcttac ccgaccccga cacgccggtg aacaccgtca ggcgccgctt
     1261 gggcagttcg acgtccatgt tcttgaggtt gttctcgcgc gccccgtgga cccgaatgcg
     1321 gtcgtggcga tcagcggcat ccccgcccgt ccgttcgctc gtcgtactcg tctgtggtgt
     1381 ggagctcatg gcccagattg aaccagcacc ggttgccgcg atggaccgcg gccggtccgc
     1441 ggctggtccg cggccggtcc gtgggtagac ggcagaccca tttcgtcata aggtgccata
     1501 atcatccgac ggcgacttgc cgccggttcg attgggagga ctcaccacca tggacgctgt
     1561 tctggacgcc gtcatcgacg acacgatcgc cgtcaccgct gccgacacca ctgctgccga
     1621 caccaccgct gccgacacca ccgctgccgc gaccaccgcg gtcgagtcgc tggtcgagga
     1681 cgaactcctc atcgaggaga tttccatcga cggcatgtgc ggtgtctact gatcacgcgt
     1741 tgtcgagcgt gctcgacgcc gcgctcacgc tgagccccca ggtcgcgctg cggcccgaac
     1801 ccttcggcgc gctcgcgtat cactacggca accgccgtct cgtcttcctc cgccacccgg
     1861 acatggtccg tgtcgcgacg gccctggccg aacaccccac gctgcgagca accctcgaag
     1921 cctgcgccat cgcaccgcca cgctggccca gcttcgagcg ggcgatcgcg tccctgcaga
     1981 cctcggaggt catctgtgag cgctgacacg ctcgtctccc aactcaagcg cggcctcgat
     2041 gcaccgattt gtctcacctg ggagctcacc tacgcctgca acctgcagtg cgtgcactgc
     2101 ctgtcgtcga gcggacagcg cgacccgcgc gaactcacca ccgccgaagc caagcgggta
     2161 ctcgacgagc tggccgagct gcaggtgttc tacatcaaca tcggtggcgg cgagccgatg
     2221 gtgcgccgcg acttcttcga actgatcgag tactcggtgg ccaagggcat cggggtcaag
     2281 ttcagcacca acggcgcgtt catcgacact gccaaggccg ctcgcctcgc ggcgatggac
     2341 tacctcgaca tccagatcag cctcgacggc accgacgccg ccaccaacga cgccgtgcgc
     2401 gggccgggca gctacgacat ggcgatccgg gcgatgcagc acctgcgcga cgccaacttc
     2461 ggaccgttca agatcagcgt ggtcgtcacc cgccacaacg tcgaccagct cgacgagttc
     2521 aaggccctcg ccgatcgcta cggcgcccag ttgcgcatca cgcgcctgcg cccgagcggt
     2581 cgcggtgccg acacctggca cgagctgcac cccaccaacg cacagcaacg gcagatctac
     2641 gactggctga tggccaacgg cgagcaggtg ctcaccggcg actcgttctt ccacctcaac
     2701 gccctcggcg
//
LOCUS       DAORNF010000468         2704 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_28477_length_2704_cov_1.365421, whole genome shotgun sequence.
ACCESSION   DAORNF010000468 DAORNF010000000
VERSION     DAORNF010000468.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2704
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28477_length_2704_cov_1.365421"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(76..321)
                     /locus_tag="PLV68_16085"
     CDS             complement(76..321)
                     /locus_tag="PLV68_16085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02821.1"
                     /translation="MSIEIGNISVQYRVSFGKKDEVVEGADDAHVVLTVAAADCGLDP
                     AVAFMRGKLKSAGSTGAFFALLADGSLGPTLRRLASR"
     gene            complement(318..>697)
                     /locus_tag="PLV68_16090"
     CDS             complement(318..>697)
                     /locus_tag="PLV68_16090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02822.1"
                     /translation="LELRGTSPYDESWFSRRSNHDYRITSKIDVGKYLWARSGALRAH
                     ATQVDPTESFWFGLADDELAEVYPYEDWCLTASRVGPVPVGEDPDHPVPGDEVEFDLF
                     AGIAPVVSALRALPAPQARETHS"
     assembly_gap    698..972
                     /estimated_length=275
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<973..>1179)
                     /locus_tag="PLV68_16095"
     CDS             complement(<973..>1179)
                     /locus_tag="PLV68_16095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02823.1"
                     /translation="GGEEGDLQNPALREPGQPLHGLTPEQEKAAIAAMRPAELAESAR
                     IIGFDEVVMLGYRDSGMADTPPNQ"
     assembly_gap    1180..1328
                     /estimated_length=149
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1329..2131
                     /locus_tag="PLV68_16100"
     CDS             <1329..2131
                     /locus_tag="PLV68_16100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HRE02824.1"
                     /translation="LVCRRLGRSRRLTAVLAGRPPLSRSALLATSLVMCLAASTALAA
                     CNDDGRTLRPARLDQNGSVYTTTTTTTTIETSLDNFGNLDGASANDYDGDSGRDGDSD
                     ANGDLDPALTTPTLAFVLNLPWPDGGTIDALYTCAGGNVQPGVSWLGAPAGAVEMALV
                     VTDTDVSTEAGNLVHWVVAGLDPTDPFIGQASLPAGAIVGLNEVDSTAGWAGPCPPAG
                     SSHHYLFSLYALDQQLELASGTPAVDLIAAIELIALSVTHRTGVYPGN"
     gene            2245..>2704
                     /locus_tag="PLV68_16105"
     CDS             2245..>2704
                     /locus_tag="PLV68_16105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HRE02825.1"
                     /translation="MTGRLVSADDSFRSLAFGGATLPVGAAPWANAHPGDRAAAELAW
                     RRLREAGEPISASFRVWTSTGKLVWLRLDTSPKRDLFGQVTGYTGCVTDITEEVHQRQ
                     LSERLSGLLEASPDAVLVIDRHGSPTFTNQAARTLFGVDDAVDLVREPAMR"
BASE COUNT          370 a          793 c          769 g          348 t
ORIGIN      
        1 agcgcccgga gacagtgttc agttgacccc gctcaccggg gtggatcgaa cacacccggc
       61 catggcgggg gtgagtcagc gactggcgag gcggcggagg gtggggccga gcgagccgtc
      121 ggcgagcagc gcgaagaagg cgccggtcga gccagccgac ttcaacttgc cgcgcatgaa
      181 ggcaacggca gggtcgagac cgcagtcagc cgcggcgacg gtgagcacga catgggcgtc
      241 gtcggcccct tcgacgacct cgtccttctt gccgaacgac acccggtact gcacgctgat
      301 gttgccgatc tcgatgctca cgaatgggtc tcccgggctt gaggggctgg caatgctcga
      361 agggctgaga cgacgggggc gatgcccgcg aacaggtcga actccacctc gtcgcctggt
      421 accggatgat cgggatcctc gcccaccggc acggggccga cacgcgatgc agtcaggcac
      481 cagtcctcgt aggggtagac ctcggccagt tcgtcgtcgg ccaagccgaa ccagaacgac
      541 tcggtgggat cgacctgggt cgcgtgggca cgcagtgccc ccgagcgggc ccacaggtac
      601 ttgccgacgt cgatcttgga cgtgatgcga tagtcgtggt tggaccgccg cgagaaccac
      661 gactcgtcgt acggcgaggt gccccgcagt tcgagcannn nnnnnnnnnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      961 nnnnnnnnnn nngttggttg gggggcgtgt cggccatgcc cgagtcgcgg tagcccaaca
     1021 tcaccacttc gtcgaagccg atgatgcgtg ccgactcggc cagttcggcc gggcgcatgg
     1081 ccgcgatcgc cgccttctcc tgctcggggg tcagcccatg caacggctgg ccgggttcgc
     1141 gcagggccgg gttctggaga tcgccctctt cgccgccggn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnngg ctggtctgcc gacgactcgg ccgatctcgt aggctcaccg ccgtgctcgc
     1381 tggccgtccg cccctgtccc gatccgcgtt gctggccacg agtctggtca tgtgcctggc
     1441 cgcctcgact gcccttgccg cgtgcaacga cgacggccgc accctgcgcc ccgctcgact
     1501 ggaccagaac gggtcggtgt acaccaccac cacgaccacg accacgatcg agaccagcct
     1561 cgacaacttc ggcaacctcg acggcgcctc tgcgaacgac tatgacggtg acagcggtcg
     1621 tgacggtgac agcgacgcga atggcgatct cgacccggcg ctgaccacac cgacgctggc
     1681 cttcgtactc aatctgccgt ggcccgacgg ggggacgatc gatgcgctgt acacctgtgc
     1741 cgggggcaac gtgcaacccg gcgtgagttg gctcggcgcc ccggccgggg cggtcgagat
     1801 ggcgctcgtc gtcaccgaca ccgacgtcag caccgaagcg ggcaacctgg tgcactgggt
     1861 cgtcgccggg ctcgacccga ccgatccgtt catcgggcag gcgtcgctgc ccgccggcgc
     1921 gatcgtcggg ctcaacgagg tcgacagcac ggcgggctgg gccgggccgt gcccacccgc
     1981 cggatcgagc caccactacc tgttcagcct gtacgccctg gaccagcagc tcgagctggc
     2041 gtccggcacc ccggccgtcg acctgatcgc agcgatcgag ctgatcgcgc tctccgtgac
     2101 gcaccgaaca ggcgtctacc ccggaaattg acgagaactt gagccttcgc gggaccggtt
     2161 cggctagaca tgagatcggt tcgcgacggg gaagggacag ctctcagcat gacctcactc
     2221 ccggtaggga tgttcgagac agacgtgaca ggacggctcg tgtcagccga cgattcgttt
     2281 cgctcccttg cattcggtgg cgcaaccctg cccgtcgggg ctgccccgtg ggccaacgcc
     2341 cacccgggag accgggccgc cgccgaactg gcgtggcgac gcttgcgcga ggcgggtgag
     2401 cccatttcgg ccagcttccg ggtgtggacc agcaccggca agctggtctg gctgcgcctc
     2461 gacaccagcc ccaagcgcga cctgttcggc caagtcaccg gctacaccgg gtgtgtcacc
     2521 gacatcaccg aagaggtcca ccagcgccag ctcagcgagc gtctcagcgg tctgctcgaa
     2581 gccagccccg acgccgtgct ggtcatcgac cgccatggct cgccgacgtt caccaaccag
     2641 gccgcacgca ccctgttcgg cgtcgacgac gcggtcgacc tggtgcgcga accagcgatg
     2701 cggt
//
LOCUS       DAORNF010000469         2694 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_28604_length_2694_cov_1.715801, whole genome shotgun sequence.
ACCESSION   DAORNF010000469 DAORNF010000000
VERSION     DAORNF010000469.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2694
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28604_length_2694_cov_1.715801"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>313
                     /locus_tag="PLV68_16110"
     CDS             <1..>313
                     /locus_tag="PLV68_16110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02826.1"
                     /translation="NVYLDQSELERADDHFRQGHVMAQQLHDVWHMAAFANLRATVAA
                     AGADPLVAADWHRAAMSGWAAGGFHSYATLAATELATALARGGRLDEAAAVLFGRRLQ
                     YI"
     assembly_gap    314..406
                     /estimated_length=93
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <407..790
                     /locus_tag="PLV68_16115"
     CDS             <407..790
                     /locus_tag="PLV68_16115"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HRE02827.1"
                     /translation="LRQALGVPFRPYFLDVLARQRDALRDALTSPVFARVWSEGTALT
                     LPDALAAVATFLDQAPPTGRLSAREREVLQLVAQGASDGEIAARLYISRHTASKHVAS
                     VLAKLDAPNRTAAVAAAYRRGLIAP"
     gene            1002..>1516
                     /locus_tag="PLV68_16120"
     CDS             1002..>1516
                     /locus_tag="PLV68_16120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02828.1"
                     /translation="MAPAATSAAPSASARAANVPRTEHVADVANVLAAEPAPTGAVLT
                     VDGQSGNVVYAQRGGTQRFDVGDLLDMASLVPDFINASNPDPDFYNFNGWWTSNEFLV
                     YPNATCSGQFVAEFYNNHMQPSSATDSASFSGAVRTYSVRASQTLWTWWLEASIDIVD
                     TVDEAMTTPAHA"
     assembly_gap    1517..1628
                     /estimated_length=112
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1629..>1952
                     /locus_tag="PLV68_16125"
     CDS             <1629..>1952
                     /locus_tag="PLV68_16125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02829.1"
                     /translation="LQTTQETGAGWRQQLVQTDSSCVDGTTIAESGVGQTVGITITRP
                     PGAYWFMVRAVQGTDEIYTNCVQVTVQDVAATLKVDGHSDTATFLPGQSVALYVDGFT
                     PGAKLT"
     assembly_gap    1953..1965
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2077..>2694
                     /locus_tag="PLV68_16130"
     CDS             2077..>2694
                     /locus_tag="PLV68_16130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02830.1"
                     /translation="MQFISSNAAGPNEWTNCVALTTSAEAVVLRVNGSLGPVQGDGGE
                     IHLVATGFPVGSQVYPVLGCEGDFEPDDFGSVVGESRTVEFFLNDDLPTDYFFVRVAM
                     APPSQVSSNCVLVRVVDYLIDPVITIDGSESPDPVVEGTPVDVVGAAFPPERTVTVRT
                     YPASGDCTGDFVTGGAESTDEGDLTFATAATSAAVAGGAMSFQVVV"
BASE COUNT          376 a          833 c          849 g          418 t
ORIGIN      
        1 aacgtctatt tggatcaatc cgaactggag cgggccgacg atcacttccg ccagggccac
       61 gtcatggccc agcaactgca cgacgtgtgg cacatggcgg cctttgccaa cctgcgcgcc
      121 acggttgctg ccgccggtgc cgacccgctg gtcgcggccg actggcatcg cgccgcgatg
      181 agcggctggg cggccggtgg cttccacagt tacgcgacgc tggccgcgac cgagttggcc
      241 actgcgctgg cccgcggggg gcgactcgac gaggccgcgg cggtcttgtt cggccggcgc
      301 ctgcagtaca tcgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnctgc gtcaggcgtt
      421 gggcgtgccc ttccgcccct acttcctcga cgtgcttgcc cggcagcgcg atgcactgcg
      481 cgatgcgctc acctcgccgg tcttcgcccg cgtgtggagc gaggggaccg cgctcacgct
      541 gcccgacgcg ctcgccgcgg tggcgacttt tctcgaccag gcaccgccca ccggacggct
      601 ttcggcgcgc gagcgcgagg tgctgcagct cgtggcccag ggcgcgtccg acggcgagat
      661 cgcggcgcgg ctgtacatct cccggcacac ggcgtcgaaa cacgtcgcgt ccgtgttggc
      721 caagctcgac gcccccaacc gcactgccgc cgtcgccgcc gcctatcgcc gcggcctcat
      781 cgcgccgtga cgttctgagc gtggtgttcg tgccgtgatg tcctggccgg cgacgcatcg
      841 gacagaaaat ggtgagaact actactttcc cgcacccccg cgaccgtcca agctgacggc
      901 tgatcgcctg gacgtgagga gatgccatgg tgcgagaggg tcgtttcagg ccgggtgcga
      961 cgctggctgt tgcgacgttc gcgttgtgga cggtgacgac ggtggcaccg gctgcaacct
     1021 cggccgcgcc gtcggcttcg gcccgagcgg cgaatgtccc tcgcaccgag cacgtcgcgg
     1081 acgtcgccaa cgttctcgcc gcggagcccg cgccgaccgg cgcggtgctg acggtcgacg
     1141 gtcagagcgg caacgtcgtc tatgcccagc gcgggggtac ccaacggttc gacgtgggcg
     1201 acctgctcga catggcctcg ctggtacccg acttcatcaa cgccagcaac cctgaccccg
     1261 acttctacaa cttcaacggc tggtggacgt cgaacgaatt cctcgtctac cccaacgcca
     1321 cttgttccgg tcagttcgtg gccgagttct acaacaacca catgcagccg tcgtcggcaa
     1381 cggacagcgc gagcttcagc ggggcggtgc ggacctactc ggtgcgggcc agccagacgt
     1441 tgtggacgtg gtggctggag gccagtatcg acatcgtcga caccgtcgac gaggcgatga
     1501 cgactccggc ccatgcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnngc tgcagaccac gcaagagacc ggcgcggggt ggcgtcagca actggtccag
     1681 accgacagca gttgcgtcga cggcaccacg atcgccgagt ccggagtggg gcagaccgtc
     1741 gggatcacga tcacccgccc gcccggtgcc tactggttca tggtcagggc cgtgcagggc
     1801 accgacgaga tctacaccaa ctgcgtacag gtgacggtgc aggacgtggc ggcgacactg
     1861 aaggtcgacg gccactccga cacagccacg ttcctccctg gacaatcggt ggcgttgtac
     1921 gtggacgggt tcacgccagg tgcgaagttg acnnnnnnnn nnnnnccgct cagtgcgacg
     1981 tgcagtggct tgtcggcggc gctctccttg ggcaacgccg acgcggaggg cacgttcgag
     2041 acgtccgcga ttatcgggta cgaccacggt gtgtcggtgc agttcatctc tagcaatgct
     2101 gcggggccga acgagtggac caactgtgtc gcgctgacga ccagcgccga agctgtcgtg
     2161 ctgcgtgtca acggcagcct tggtcccgtg cagggcgacg gtggcgagat ccatctcgtg
     2221 gccaccggct ttccggtcgg gtcacaggtg tatccggtgc tcggctgcga gggtgacttc
     2281 gaacccgacg acttcggctc ggtcgtcggt gagagtcgca cagtcgagtt cttcctcaac
     2341 gacgacctgc cgaccgacta cttcttcgtg cgggtcgcga tggccccgcc gtcgcaggta
     2401 tcgagcaact gcgtgctcgt ccgcgtcgtg gactacctga tcgatccggt gatcaccatc
     2461 gacggcagcg agtcgcccga tccggtggtc gaggggacgc cggtcgatgt ggtcggcgcg
     2521 gcgttcccgc ccgagcggac ggtgacggtt cgcacctacc cggccagcgg cgactgcacg
     2581 ggcgacttcg tgaccggagg agccgagagc acggacgagg gcgatctgac cttcgcgacg
     2641 gccgccacct cggccgcggt ggcgggtggt gcgatgtcgt tccaggtggt cgtc
//
LOCUS       DAORNF010000470         2693 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_28618_length_2693_cov_1.854056, whole genome shotgun sequence.
ACCESSION   DAORNF010000470 DAORNF010000000
VERSION     DAORNF010000470.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28618_length_2693_cov_1.854056"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>287)
                     /locus_tag="PLV68_16135"
     CDS             complement(<1..>287)
                     /locus_tag="PLV68_16135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02831.1"
                     /translation="STVTPGPKSTTFGDLDLICARYPWYSTTAAAANVPPVNAADWGD
                     WLLARTTKRPQVPDGWDDWDGWQFSAGYNGRGPTYGATSADLDLNIVRPEA"
     assembly_gap    288..297
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<298..713)
                     /locus_tag="PLV68_16140"
     CDS             complement(<298..713)
                     /locus_tag="PLV68_16140"
                     /inference="COORDINATES: protein motif:HMM:NF013357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH25 family lysozyme"
                     /protein_id="HRE02832.1"
                     /translation="MIPIIDVSKWQGDIDFTRAARNGVRGVVVRAGNGTAADARCGEY
                     IAGARKAGLVVGVYWFCNPKRSSGADQGQRLADAHNLHRCELPPMLDVEDYTGEPGTL
                     PVLSPAQFTTWLTAMRVKIESAVGRAGRAHGHDGAAG"
     gene            complement(739..1443)
                     /locus_tag="PLV68_16145"
     CDS             complement(739..1443)
                     /locus_tag="PLV68_16145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02833.1"
                     /translation="MIGTTDLARLLTPAPAETVDYQYGTVTAVSPLTVRVDSETVGVV
                     TASVIAIPFSVNDRVLVLVRGADRTVIASASSPVTITPYARTLLDDADAPTARATLGV
                     GGVWSSWSHNWTASGGGSSLGNGTASARYTRIVDSVTVQLAMKFGSTTNFGSGAFLWN
                     LPVTAAIPGTDLGPLFTGYAYDSSANLMRAVLGYCVNAWTIACYVDNGGAVSQGTPWT
                     WANADQIKLFGTYEAA"
     gene            complement(1440..>1918)
                     /locus_tag="PLV68_16150"
     CDS             complement(1440..>1918)
                     /locus_tag="PLV68_16150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02834.1"
                     /translation="TSNTSTAWTIGKTSETERSVLIDISEKKARAPIYNRATVVGVNA
                     SNTAQYRGVATDTDPTSLTQYGGPFGKKPMPPVTSSLIGSQAQAVAASQALLTSQLGS
                     GQSLIVDAVPNEALEAGDPVQIVRTEMGIDQVHLADVIRLGLGPGGVQRIETRRAA"
     assembly_gap    1919..2040
                     /estimated_length=122
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2041..>2286)
                     /locus_tag="PLV68_16155"
     CDS             complement(<2041..>2286)
                     /locus_tag="PLV68_16155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02835.1"
                     /translation="GLRYGDGTIETMSLGIFPIQDATAGAMLDRKIVATDRSQWVIDA
                     IFEDATAITAGTNYADAIKTVITGGYPAAQFAFATTT"
     assembly_gap    2287..2406
                     /estimated_length=120
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          394 a          803 c          819 g          425 t
ORIGIN      
        1 cacgcttcgg ggcgcacgat gttcagatca agatcggccg aggtggcgcc gtaggtgggg
       61 ccgcggccgt tgtatccggc cgagaactgc cagccatccc agtcgtccca gccgtccggc
      121 acctgcggcc gtttcgtggt gcgggcgagg agccagtcgc cccagtcggc ggcgttcacc
      181 ggggggacgt tcgccgccgc ggcggtggtg gagtaccagg ggtagcgggc gcagatcagg
      241 tcgaggtcgc cgaaggtggt gctcttgggg ccgggcgtca cggtgctnnn nnnnnnnccg
      301 gcggccccgt cgtgcccatg ggcacgcccg gcacgcccga cggcggactc gatcttcacg
      361 cgcatggccg tcagccacgt cgtgaactgg gcgggactca agactgggag ggtgccgggc
      421 tcgccggtgt agtcctcgac gtcgagcatc ggcgggagtt cgcagcggtg caggttgtgt
      481 gcgtcggcga gccgctgccc ctgatccgct cccgacgacc gcttcgggtt gcagaaccag
      541 tacacaccca cgaccagtcc ggctttgcgg gcaccggcga tgtactcgcc acaccgcgcg
      601 tcggccgcgg tgccgttccc agcgcgcacg accacaccac gcacgccgtt gcgggcggcc
      661 ctggtgaagt cgatgtcgcc ctgccacttc gacacgtcaa tgatcgggat catgtaggtc
      721 ttcctttcgt ttgcaacatc acgcggcctc ataggtgccg aagagcttga tctggtcagc
      781 gttcgcccac gtccacggcg taccctgcga gaccgcgccg ccgttgtcga cgtaacatgc
      841 gatcgtccac gcattgacgc aatacccgag caccgcccgc atcaggttcg ctgacgagtc
      901 atacgcgtaa ccggtgaaca acggcccgag gtcggtccca gggatggcgg cagtgaccgg
      961 caagttccac aggaaagcgc ccgatccgaa gttcgtcgtt gatccgaact tcatcgcgag
     1021 ttgcacggtc acgctgtcga cgattcgcgt gtaccgcgct gaggccgtcc cgttgcccag
     1081 tgatgaaccg ccaccagacg cggtccagtt gtgcgaccac gacgaccaca ctccacccac
     1141 acccaacgtc gcccgcgctg tcggcgcgtc cgcatcgtcg agcagggtgc gagcatatgg
     1201 cgtgatggtc accggggatg acgccgacgc gatcaccgtc cgatcagcac cacgcaccaa
     1261 caccaacacc cgatcgttga cactgaacgg gatagcgatc accgaggccg tcacgacacc
     1321 gaccgtctcc gagtcaactc ggacggtcag cggcgacacc gcggtcactg tcccgtactg
     1381 gtagtcgacc gtctctgccg gtgctggtgt cagcagccgg gcgaggtccg tggttccgat
     1441 catgctgccc tcctcgtctc gatccgctgc actccaccgg gacccaaccc gaggcggatc
     1501 acgtcggcga ggtggacctg atcgatcccc atctcggtgc gcacaatctg caccggatca
     1561 cccgcctcga gcgcctcgtt cgggacggca tcgacaatga gcgactgccc cgaccccagc
     1621 tgcgatgtga gcagcgcctg cgaagcagca acggcctgcg cttgggaacc gatcagggat
     1681 gacgtgacag ggggcatcgg cttcttcccg aacgggccgc cgtactgcgt cagcgaggtc
     1741 gggtcagtgt cggtcgcgac accccggtat tgcgcggtgt tcgacgcgtt gacgccgacg
     1801 acggtggccc ggttgtagat cggtgctcgc gccttcttct cggagatgtc gatcagcacg
     1861 gaccgttcgg tctcgctggt cttgccaatc gtccacgccg tgctggtgtt cgatgtgcnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 aacgtggtcg tcgcgaacgc gaactgggcg gcggggtagc cgccggtgat gacggtcttg
     2101 atcgcgtcgg cgtaattggt acccgcggtg atcgcggtgg cgtcctcgaa gatggcgtca
     2161 atcacccatt gggatcgatc ggtggcgacg atcttgcggt cgagcatcgc gcccgcggtc
     2221 gcgtcttgga tcgggaagat gccgagcgac atggtctcga tggtgccgtc gccgtagcgc
     2281 agaccgnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnggtg atggtgccgt cgtccccccg taggccggtg aggatcgtcg atccggcgta
     2461 aaggatgtcg gcgacgacga cgagctcgcg agatccgcgt accgcggtgt cccaccgcga
     2521 tgaacgtgcc ctcacgaggt cggcctcgcg atctcgatga acgcaagttc ccagaaccgg
     2581 tgcgggcgga ccttttggat gaggtgctgt tcgcgccagc gtccggacgc gatgtagcgg
     2641 tcgggggtgt agtcccacgt tgccggcatc tgcagtagca ggaacggccg gtc
//
LOCUS       DAORNF010000471         2685 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_28726_length_2685_cov_2.037643, whole genome shotgun sequence.
ACCESSION   DAORNF010000471 DAORNF010000000
VERSION     DAORNF010000471.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2685
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28726_length_2685_cov_2.037643"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>290
                     /locus_tag="PLV68_16160"
     CDS             <1..>290
                     /locus_tag="PLV68_16160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HRE02836.1"
                     /translation="LVAALREGMLRLAGREADGVIINWLSPDDVTKVVGVVRHAAGGD
                     EREIVARIFVCPSENADVVRAGARFAIAAYLNVPVYAQFHDWLGRGDLLRPM"
     assembly_gap    291..337
                     /estimated_length=47
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <338..508
                     /locus_tag="PLV68_16165"
     CDS             <338..508
                     /locus_tag="PLV68_16165"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HRE02837.1"
                     /translation="EVVDQLIVHGSPERCRATIDRYFANGVTTTSLAIMPFDPELKHW
                     DAVRLLAPNQPT"
     gene            complement(551..769)
                     /locus_tag="PLV68_16170"
     CDS             complement(551..769)
                     /locus_tag="PLV68_16170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02838.1"
                     /translation="MTEHTSTQPEQLRLLDGSDVPLAFRLDERTRRRGLAHIAELRTI
                     IARQAAARHLSAETQAELHAVGSRRPAA"
     gene            complement(893..1699)
                     /locus_tag="PLV68_16175"
     CDS             complement(893..1699)
                     /locus_tag="PLV68_16175"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HRE02839.1"
                     /translation="MQAAVGSHCVDCVKASRPPVATRVRAVNARQHTLVTNVIIALNG
                     LIFLWVALGDPSTLGNGGSISKRQFELGLDRLILQYGARYVETSRGPTILIDPDRAHE
                     WYRLATSGFLHFGIIHLLLNMYLLYLLGQILERSLGRTRFILLYVASLLGGSAGVILM
                     TEGSVTGGASGAVFGLMGAATIAMWRQGVNILQTGVGRTLVLNLIITFALGSSLNISI
                     GGHLGGLVAGAACGAVMLAPKWRPIPRWATYATPVAVAVLAVVIAVGRVG"
     gene            complement(1912..>2685)
                     /locus_tag="PLV68_16180"
     CDS             complement(1912..>2685)
                     /locus_tag="PLV68_16180"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRE02840.1"
                     /translation="IKAGLDAGDFFVVYQPIVSTHTGRMIAAEALLRWHHPERGLLLP
                     REFLHLAEESGMIVPVGEWVVRQACRDARAWIDAGLVERAFSVHVNVSGRQLTDGAFV
                     ERVINTIRELDLLPQQLTLDFDEHTLFGDQPSTLRAIQALRRFGVAIALDSFGTGVSS
                     LTALRDSTADVLKLDGTIASTLGDIDGNNHDPIVRSIIQLAHALDMEVVAEWVTAPDQ
                     LYRLKMLGCDMAQGFLFGEPCAAETFAETAAEAALGRRG"
BASE COUNT          431 a          851 c          944 g          412 t
ORIGIN      
        1 cctggtcgcg gcgctgcgcg agggcatgtt gcgcctggcc gggcgcgagg ccgacggggt
       61 gatcatcaac tggctgtcgc ctgacgacgt gaccaaggtg gtcggtgtcg tgcgccacgc
      121 cgctggtggc gacgagcgtg agatcgtggc ccgcatcttc gtctgtccca gcgagaacgc
      181 agacgtcgtg cgcgccggcg cccgattcgc gatcgcggcg tacctgaacg tgccggtgta
      241 cgcccagttc cacgactggc tcggccgcgg cgacctgctt cgcccgatgt nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngaa gtggtcgatc agttgatcgt
      361 gcacggttca cccgaacgct gccgggccac gatcgaccgc tacttcgcca acggcgtgac
      421 cacgacctcg ctggcgatca tgccgttcga tcccgaactg aagcactggg atgccgtgcg
      481 cttgctggcc cccaatcagc caacatgaac cagccggggt gggctggatc ggcctgatcg
      541 gacgggtggg tcaggccgcg gggcggcgtg acccaaccgc gtgcagttcg gcctgggtct
      601 cggccgacag atgccgcgcc gccgcttggc gggcgatgat ggtgcgcagt tcggcgatgt
      661 gggccaggcc acggcgccgg gtgcgctcgt cgaggcggaa tgccaacggc acgtcactgc
      721 cgtcgagcaa ccgcagttgt tcaggctggg tggaagtgtg ctcggtcatg attgacatga
      781 tgaaccaggg gtgtcacgcg gtttgttccg cacccgtgcg gcgatgtgac gcctgaccag
      841 tgataccggg ccggtatcgc gtcgggttgt ggtgccgtgg ctgggaacag cgtcagccga
      901 cgcggccgac cgcgatcacc acggcgagca ccgcaacggc aaccggtgtg gcatacgtgg
      961 cccagcgggg gatcggccgc cacttgggag ccagcatcac cgcgccgcac gcggcaccgg
     1021 cgacgagccc gccgaggtgt ccgccgatgg agatgttcag cgacgagccg agggcgaagg
     1081 tgatgatcag gttgagcacc agcgtgcgcc cgaccccggt ctggaggatg ttgacgccct
     1141 ggcgccacat cgcgatcgtg gctgcaccca tcagcccgaa cacggcaccc gaagccccac
     1201 cggtgacgct gccctcggtc atcaagatga ccccggccga cccaccgagc aacgaggcca
     1261 cgtacagcag gatgaaccga gtgcgcccca gggaacgctc gaggatctgg cccagcagat
     1321 acagcaagta catgttgagc agcaggtgga tgatgccgaa gtgcaggaat ccgctggtcg
     1381 ccaggcgata ccactcgtgc gcgcggtccg ggtcgatgag gatcgtcggg ccacgcgatg
     1441 tctcgacgta ccgcgctccg tactgcagga tcagccgatc cagcccgagc tcgaactgtc
     1501 gcttcgagat cgagccaccg ttgccgagtg tggacggatc gcccaacgcc acccacagga
     1561 atatcaaccc gttgagggcg atgatgacgt tggtgaccag cgtgtgctgg cgggcgttga
     1621 cggcccggac gcgggtggcg accggcggcc gcgacgcctt gacgcagtcg acgcagtgcg
     1681 agccgaccgc ggcctgcacg aggcagtcgt tgcaggcgat gttgccgcaa cgggtgcagc
     1741 tccgccacgc ctcacgatcg ggatggcggt agcagcgggt tctctccggg gtcggcgcca
     1801 ccggtgacga cgaaggcggt gggggcaacg gcatgcgggc tcagggtatc gggtgcgatg
     1861 tggccagatc gggcagcacg ggtcggccag ttgtactcag cccagcccgg ttcagccgcg
     1921 ccggccgagc gccgcctcgg ccgcggtctc ggcgaaggtc tctgccgcgc acggctcacc
     1981 gaacaggaag ccctgggcca tgtcgcaacc cagcatcttc agccggtaca gctgatcggg
     2041 tgcggtgacc cactcggcga ccacctccat gtcgagcgcg tgggcgagtt ggatgatcga
     2101 gcgcacgatc gggtcgtggt tgttgccgtc gatgtcgccg agcgtcgatg cgatcgtgcc
     2161 gtccagcttc aacacgtcgg cggtcgagtc gcggagtgcc gtcagcgacg agacgcccgt
     2221 gccgaagctg tcgagcgcga tcgccacgcc aaagcggcgc agtgcctgga tcgctcgcag
     2281 cgtggacggc tggtcgccga acagggtgtg ctcgtcgaag tcgagcgtga gctgttgcgg
     2341 cagcagatcg agttcgcgga tcgtgttgat gacccgttcg acgaaggcgc cgtcggtgag
     2401 ctggcggccg ctgacgttga cgtggacgct gaaggcccgt tcgaccagtc cggcgtcgat
     2461 ccacgctcgg gcgtcgcggc acgcctggcg cacgacccac tcgccgaccg gcacgatcat
     2521 gccgctctct tcggccaggt gcaggaactc gcgcggtagc aacagtcccc gctcgggatg
     2581 gtgccagcgc agcagagcct cggcggcgat catccgtccg gtgtgggtgg acacgatcgg
     2641 ctggtagacg acgaagaagt cgcccgcgtc gagccccgcc ttgat
//
LOCUS       DAORNF010000472         2674 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_28860_length_2674_cov_1.150821, whole genome shotgun sequence.
ACCESSION   DAORNF010000472 DAORNF010000000
VERSION     DAORNF010000472.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2674
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28860_length_2674_cov_1.150821"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..159)
                     /locus_tag="PLV68_16185"
     CDS             complement(<1..159)
                     /locus_tag="PLV68_16185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRE02841.1"
                     /translation="MIRLDNVTKIYNSEVAAVRDATFEINKGEFVFLVGPSGSGKSTF
                     IRLLNKEET"
     gene            complement(257..>426)
                     /gene="prfB"
                     /locus_tag="PLV68_16190"
     CDS             complement(257..>426)
                     /gene="prfB"
                     /locus_tag="PLV68_16190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442394.1"
                     /note="recognizes the termination signals UGA and UAA
                     during protein translation a specificity which is
                     dependent on amino acid residues residing in loops of the
                     L-shaped tRNA-like molecule of RF2; in some organisms
                     control of PrfB protein levels is maintained through a +1
                     ribosomal frameshifting mechanism; this protein is similar
                     to release factor 1; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HRE02842.1"
                     /translation="GWGSQIRSYVLQPYQMVKDLRTEVESSNVAGVLDGDVDQFMEGY
                     LRHRRRMADEH"
     assembly_gap    427..443
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<444..>1075)
                     /locus_tag="PLV68_16195"
     CDS             complement(<444..>1075)
                     /locus_tag="PLV68_16195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PCRF domain-containing protein"
                     /protein_id="HRE02843.1"
                     /translation="EPDIDAAIAAASGALSQLELRSLFTGEHDELDCIVQINAKDGGV
                     DAQDWSEMLLRMYGRWAERRGLGFEVDGISEGSEAGILSAEFTLTGRYAYGLMTSERG
                     THRLVRISPFDNQGRRQTSFAAVQVWPVLDDPDVDINEADIRMEVFRASGAGGQHINK
                     TSSAVRLIHEPTGVVASSQEERSQLQNREKAMNRLRTMIAARVLEERQAE"
     assembly_gap    1076..1279
                     /estimated_length=204
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1398..>1566)
                     /locus_tag="PLV68_16200"
     CDS             complement(1398..>1566)
                     /locus_tag="PLV68_16200"
                     /inference="COORDINATES: protein motif:HMM:NF014825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HRE02844.1"
                     /translation="VAGEVAAEAAAAESAPAAAVKQAPRVKDEWAKTSRNAPCPCGSG
                     KKFKACHGANV"
     assembly_gap    1567..1667
                     /estimated_length=101
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1668..>1899)
                     /locus_tag="PLV68_16205"
     CDS             complement(<1668..>1899)
                     /locus_tag="PLV68_16205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02845.1"
                     /translation="RREQELTPKVMRDVERQVMLRIIDQRWREHLEEMDYLQEGINLR
                     AMGQKDPLVEWQREGFELFGLLMKGVAQDFVR"
     assembly_gap    1900..2039
                     /estimated_length=140
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2040..>2304)
                     /locus_tag="PLV68_16210"
     CDS             complement(<2040..>2304)
                     /locus_tag="PLV68_16210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02846.1"
                     /translation="GRALPDDEAIEAKMVTKAIERAQGTVEQRNGEIRKNVLKYDEVM
                     NEQRKVIYQRRDQILEGADLKAGALEYLSSAAEALVATHCVSDA"
     assembly_gap    2305..2455
                     /estimated_length=151
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2456..>2674)
                     /locus_tag="PLV68_16215"
     CDS             complement(<2456..>2674)
                     /locus_tag="PLV68_16215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02847.1"
                     /translation="EGLARAETLKDFPAEVLVDDFDLPMPLEAMPGEFQLQRKQAFAR
                     YDELLAKFAVECKAEGDHVRELGGLYVL"
BASE COUNT          312 a          695 c          665 g          389 t
ORIGIN      
        1 cgtctcctcc ttgttgagga gccggatgaa ggtggacttg ccggagcccg acgggccgac
       61 gaggaagacg aactcgccct tgttgatctc gaaggtcgcg tcgcgaaccg cggcgacctc
      121 ggagttgtag atcttggtga cgttgtcgag gcggatcaaa acggctcctc agccccggtc
      181 ggtatgaccg gtggcgggca catcggggac ggcaaaagtt accagtgtgt gacgaaaagg
      241 ggtgggcacc cggacatcag tgctcgtcgg ccatgcggcg acgatggcgg aggtaaccct
      301 ccatgaactg gtcgacatcg ccgtcgagca cacccgcgac attgctggac tcgacctcgg
      361 tgcgcaggtc cttgaccatc tggtacggct ggagtacata gctgcggatc tggctgcccc
      421 acccgannnn nnnnnnnnnn nnnctcggcc tggcgctctt cgagcacccg ggccgcgatc
      481 atggtgcgca gccggttcat cgccttttcc cggttctgca gttggctgcg ctcttcctgt
      541 gagctggcca ccacgccggt cggctcgtgg atcaggcgca ccgccgagga cgtcttgttg
      601 atgtgctggc cgccggcgcc gctggcccgg aagacctcca tccggatgtc ggcttcgttg
      661 atgtcgacgt cagggtcgtc gagcaccggc cacacctgca ccgcggcaaa gctggtttga
      721 cgccgcccct ggttgtcgaa cgggctgatc cgcacgaggc ggtgagtgcc tcgctcgctg
      781 gtcatcaagc cgtaggcgta gcgaccggtg agggtgaact cggccgacag gatccccgcc
      841 tcgctgccct cggagatgcc gtcgacctcg aagccgagcc cacgccgttc ggcccaccgg
      901 ccgtacatgc gcagcaacat ctcgctccag tcttgggcgt cgactccccc gtccttggcg
      961 ttgatctgca cgatgcaatc gagctcgtcg tgttcgccgg tgaacaagct gcgcagctcc
     1021 agttgcgaca gcgccccgga cgcagcggcg atcgcggcgt cgatgtcggg ttcctnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnng cagatacgac gacgcctcgt cgaggcggcg gcgcacatct
     1321 ttcaagtcgt cggtgaaatc tcgcatggtc gctttcgtgg gtggccgaca gggatcggca
     1381 cggaccccaa ggcggggtca gacgttagcg ccgtgacacg ccttgaactt cttgcccgac
     1441 ccacacgggc acggtgcgtt gcgcgacgtc ttggcccact cgtccttgac gcgcggcgcc
     1501 tgcttgaccg cagcggcggg cgctgactca gcagccgccg cttcggcggc aacctcgcct
     1561 gcgaccnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntag cggacgaagt
     1681 cttgggcgac acccttcatc aacaggccga acagctcgaa gccctcgcgc tgccactcga
     1741 cgagcgggtc cttctgcccc atcgcacgca ggttgatgcc ctcttgcagg tagtccatct
     1801 cttcgaggtg ctcacgccag cgctggtcga tgatgcgcag catcacctgg cgctcgacgt
     1861 cgcgcatcac cttgggcgtc agttcttgct cgcgacgctn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc
     2041 ggcgtcgctg acgcagtggg tcgccaccag ggcctcggcc gcgctggaga ggtactccaa
     2101 cgcaccagcc ttgaggtcgg caccctcgag gatctggtcg cggcgctggt agatcacctt
     2161 gcgctgctcg ttcatcacct cgtcgtactt gagcacgttc ttgcggatct cgccgttgcg
     2221 ctgctcgacg gtgccctgtg cccgctcgat ggccttggtg accatcttgg cttcgatcgc
     2281 ctcgtcgtcg ggcagggccc ggccnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncgagc
     2461 acgtacagcc cacccagctc gcgcacgtgg tcgccttcgg ccttgcattc gacggcgaac
     2521 ttggccagca gctcgtcgta gcgggcgaag gcctgcttgc gctgcagttg gaactcgccg
     2581 ggcatcgcct cgagtggcat cggcagatcg aagtcgtcga ccagcacctc ggccgggaag
     2641 tccttcaacg tctcggcgcg ggccaaccct tcgg
//
LOCUS       DAORNF010000473         2673 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_28871_length_2673_cov_1.815890, whole genome shotgun sequence.
ACCESSION   DAORNF010000473 DAORNF010000000
VERSION     DAORNF010000473.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2673
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28871_length_2673_cov_1.815890"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>256)
                     /locus_tag="PLV68_16220"
     CDS             complement(<1..>256)
                     /locus_tag="PLV68_16220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRE02848.1"
                     /translation="EGTTNKMIAAEAGIAPGLIYYYFESKEQLFIAVFEQMYATRDQR
                     LAHYDFTEHTLHDNLATLLADSINLAREDPSFVEVFTISGR"
     assembly_gap    257..360
                     /estimated_length=104
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            508..1155
                     /locus_tag="PLV68_16225"
     CDS             508..1155
                     /locus_tag="PLV68_16225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HRE02849.1"
                     /translation="MDHGADPCLDLSTDELLTTTRAVRRRLDLSRPVSRDLVRRCLEI
                     AVQAPNGSNRQPWQWVVVDDPGQRRGVAEVYRGNYEALVGAAPAEQLAASDRDLASGA
                     HLAAHLHECPVLVVPCVAGRLDGLSAADAAGRWGSILPAVWSLQLALRSRGLGSTWTT
                     LHLHRGGERAVADVLGIPADDVTQIGLLPLAYTVGTDFRPARRGPLDEIVHWDHW"
     gene            1202..>1411
                     /locus_tag="PLV68_16230"
     CDS             1202..>1411
                     /locus_tag="PLV68_16230"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HRE02850.1"
                     /translation="MHVGLSMIFQNLSERDDRTMMCRELDIISRAEALGFESIWVPEH
                     HFAGYTVAPNVPQLLAWIAARTERLR"
     assembly_gap    1412..1471
                     /estimated_length=60
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1472..2324
                     /locus_tag="PLV68_16235"
     CDS             <1472..2324
                     /locus_tag="PLV68_16235"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HRE02851.1"
                     /translation="VLQPAGQAGHERAMLGIGRGLGRKEFDGLRLERGESRRRFTEYA
                     EAILTALETGELEYDGDLLQQPRVEIRPTPFATYRGRTFASAVSPASMTIMAELGVGL
                     MVIAQKPWDTTQAELAEYRETFRAINGYEAPKPILSMFVAAHENAEEAQRIHEVHLRR
                     SMASITDHYGFADTSFALVEGYEYYAKLAANIAKHGVDAFNAFLAGLQVTGTPDQVTE
                     QLLDYARRIDAGAVLATLSYGDMNAEQAGQHVDLFAREVLPHLRARDAGGDVGVRYGA
                     GLTATAG"
     assembly_gap    2450..2463
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2464..2654)
                     /locus_tag="PLV68_16240"
     CDS             complement(<2464..2654)
                     /locus_tag="PLV68_16240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02852.1"
                     /translation="MTLWGKQFRLTAERAWQLGLVDELVEPDRLVETALEMGAQVARC
                     SPAAVALSKQAIWASLGRRR"
BASE COUNT          381 a          840 c          862 g          412 t
ORIGIN      
        1 cgcggccgct gatggtgaac acctcgacga acgacgggtc ttcacgggcc aggttgatgc
       61 tgtcggccag cagcgtggcg aggttgtcgt gcaacgtgtg ctcggtgaag tcgtagtgcg
      121 ccaggcgttg gtcgcgcgtc gcgtacatct gctcgaagac cgcgatgaac agctgttcct
      181 tcgactcgaa gtagtagtag atgagaccgg gcgcgatgcc ggcctcggcc gcgatcatct
      241 tgttggtcgt gccctcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 gtcggagacg cctcagcagg tttgcggcgg gtggttgacc ggggctcagg ccgtcgagac
      421 acgcctggac tctacccaag tgacatcgac cgactccggc ttgccgtcag acgttgactg
      481 ttcggctagt caactaccat cggcgggatg gatcatggtg ccgacccgtg cctcgacctg
      541 tcgaccgacg aactgctcac cacgacgcgt gcggtgcgac gccggctcga tctgagccgc
      601 ccggtctcgc gcgacctcgt ccgccgttgt ctggagatcg ccgtgcaggc gccgaacggc
      661 agcaaccggc aaccgtggca gtgggtggtc gtcgacgatc ccgggcaacg ccgtggcgtc
      721 gccgaggtgt accgcggcaa ctacgaggcg ctggtcggtg cggcgccggc ggagcagctc
      781 gccgcgtccg accgcgatct ggcgtccggc gctcatctcg ccgcccacct gcacgagtgc
      841 ccggtgttgg tcgtgccgtg tgtggccggc cgcctcgacg gtctgtcggc tgccgatgcg
      901 gcagggcgat gggggtcgat ccttcccgct gtctggagtc tgcaactcgc gctgcgctca
      961 cgcggtcttg gttcgacctg gacgacgctg cacctccatc gcggtggcga gcgggcggtg
     1021 gccgacgtgc tgggcatccc ggccgatgac gtcacccaga tcgggctgtt gccgctggcg
     1081 tacacggtgg gcacggactt ccgtccggct cggcgaggac cgcttgacga gatcgtgcac
     1141 tgggaccact ggtgagcgcc gtacccttga attctaatct gatttagaat atgctccagc
     1201 catgcacgtg ggcttgagca tgatctttca gaacctcagc gagcgcgacg accgcacgat
     1261 gatgtgccgc gagctcgaca tcatcagccg ggccgaggca ctcggcttcg agtcgatctg
     1321 ggtacccgag caccacttcg ccggttacac ggtggcgccc aacgtgccgc agctactggc
     1381 gtggatcgcc gcgcgcaccg agcgcctgcg cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nggtgcttca gcccgcggga caggccggtc
     1501 atgaacgggc gatgctcggc atcgggcgtg gtctgggccg caaggagttc gacgggctgc
     1561 gcctggaacg gggcgagtcg cggcggcggt tcaccgaata cgccgaggcg atcctgaccg
     1621 cgttggagac gggtgagctc gaatacgacg gcgacctgct gcagcaaccg cgcgtcgaga
     1681 tccgtccgac gccgttcgcc acctatcgcg ggcgcacctt cgcctcggcg gtgtcgccgg
     1741 catcgatgac gatcatggcc gagttgggcg tcgggctgat ggtcatcgcc cagaagccgt
     1801 gggacaccac ccaggccgag ttggccgagt accgcgagac gttccgcgcg atcaacggct
     1861 acgaagcccc gaagccgatc ttgagcatgt tcgtcgcggc gcacgagaac gccgaggagg
     1921 cccagcgcat ccacgaggtc cacctccgcc gcagcatggc ctcgatcacc gatcactacg
     1981 ggttcgccga cacgtcgttc gcgctcgtcg aggggtacga gtactacgcc aagttggcgg
     2041 ccaacatcgc caagcacggg gtcgatgcgt tcaacgcatt tctcgccggc ctgcaggtga
     2101 ccggcacgcc cgaccaggtc accgagcagc tgctcgacta tgccaggcgc atcgatgcag
     2161 gcgcagtctt ggccacgctg tcgtacggcg acatgaacgc ggagcaagcc ggccagcacg
     2221 tcgacctgtt cgcccgcgag gtgttgccgc atctgcgcgc ccgcgacgcc ggtggcgatg
     2281 tgggggtccg ctacggcgct gggctcaccg ccactgccgg ctgaagcgcc gtcaccgtcg
     2341 ccggcgcttc tgtggctcgg tgggtctagt gatcggccca gcggggcggg tgcccggcgg
     2401 cgaaggccgc gccaccctcg gcgaagtccg ggtgttggcg gtgcatccgn nnnnnnnnnn
     2461 nnncggcgac gccccaacga cgcccagatc gcctgcttcg acagcgcgac cgcggccggg
     2521 gagcagcggg cgacctgcgc gcccatctcc aatgccgtct cgaccaggcg gtcgggctcg
     2581 accaactcgt cgaccaggcc gagctgccag gcccgctcgg cggtcagccg gaactgcttg
     2641 ccccacagcg tcatccgcaa caccgtgccg agc
//
LOCUS       DAORNF010000474         2672 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_28892_length_2672_cov_1.643867, whole genome shotgun sequence.
ACCESSION   DAORNF010000474 DAORNF010000000
VERSION     DAORNF010000474.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28892_length_2672_cov_1.643867"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1082)
                     /locus_tag="PLV68_16245"
     CDS             complement(<1..>1082)
                     /locus_tag="PLV68_16245"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRE02853.1"
                     /translation="YFLMMHDDVVLEPTATRILVEELYRSNAGIVGPKLVTWDDHRML
                     QHVGLGVDRFGEVDPLVEAGEIDQEQHDAVRDVFALPSACMLIRADLFRALGGFDPAI
                     TFHGDDIDLCWREHLSGARVLVVPGARARHREGLRERRTDLSHNSLAARHRVRTVATL
                     TSGVRLPLVLLQMLLISLAEMVIGLFTGRLGEAFASFKATVGLVAHTGSVVKRRRQMA
                     SLRQVPYSEVAGLQVRGSARLASYLRNREHREVTVDASSLGANRFTRNLTGQVGAWCA
                     VVALFVIGSRRFIGEGVPAVGQFLHFPDSARDLLGDYWSGWWSHGLGQTAAAPTGIGL
                     AGLSGLASIGHMGLFHTVAVLAWLPFG"
     assembly_gap    1083..1118
                     /estimated_length=36
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1119..1386)
                     /locus_tag="PLV68_16250"
     CDS             complement(<1119..1386)
                     /locus_tag="PLV68_16250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02854.1"
                     /translation="MDAKAEPALSVVAVMVVHRPTDHTAVRFDAHFDELLRALAAQDY
                     PNLKTLFLIAGEPGDLAATIRAKVPGAFVRPVGGNPGYGPAANEV"
     assembly_gap    1414..1499
                     /estimated_length=86
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1500..>2672)
                     /locus_tag="PLV68_16255"
     CDS             complement(<1500..>2672)
                     /locus_tag="PLV68_16255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02855.1"
                     /translation="VLKEWEKHPIHGAQAKNFANTLAKSMQSIVELKPGGEIKGNRAR
                     VRGDRSASINSALSSLTGWVSPETYFSWPFKVTEGGEYHIEVDQATLRDEPTRSRAWD
                     DSAKAGEEIAITFETAALGVSGPVEGAYLGSVEWGFRAPADSATAVPLPFRMISRGAP
                     SAAFLASAKLWNDNATIKANGKDTRLSIPVAFTTHQTDPLIDALLGDPSTPAERLQAA
                     IEARIRLLRSQIPGDIDKQLADLGQAKIDDQEAVSEFRDVVTALSAEWKRLKGIDTAL
                     AGAKVQADNDLRAATQNLKAIDDALNVFTRRIGGGKTTLKAAKVDVAGKRTAADKAKT
                     DSQAAAHDLDTAGQQLQAAKTDPSMQAALVTYRASLVAFQNVLADARSRQFEITAL"
BASE COUNT          407 a          863 c          870 g          410 t
ORIGIN      
        1 ccgaacggca gccacgccaa cacggcgacg gtgtggaaca gacccatgtg cccgatgctg
       61 gcgaggccgg acagaccggc aaggccgatg cccgtcggtg ccgccgcggt ctgacccagg
      121 ccgtggctcc accagcccga ccagtagtcg ccgagcaggt cgcgggcact gtcggggaag
      181 tggaggaact ggccgacggc gggcactccc tcgccgatga accgccggct gccgatgacg
      241 aacaacgcga ccaccgcgca ccaggcaccg acctggccgg tcaggttgcg ggtgaagcgg
      301 ttggcgccga gcgagctggc atcgacggtg acctcccggt gttcccggtt gcgcaggtac
      361 gacgccaggc gagcgctgcc gcgcacctgc agacccgcca cctcgctgta gggcacctgg
      421 cgcaacgatg ccatctgccg ccgtcgcttg acgaccgagc cggtgtgggc gaccagaccc
      481 acggtggctt tgaacgacgc gaacgcctcg ccgaggcgac ccgtgaacag gccgatcacc
      541 atctcggcca gcgagatcaa cagcatctgc agcaacacca acggcagacg gacgccgctg
      601 gtcaaggtcg ccaccgttcg cacccggtga cgggcagcga gcgagttgtg cgacaggtcg
      661 gtgcgccgct cgcgcagacc ctcgcggtgg cgggcacgcg caccgggcac gacgaggacg
      721 cgcgccccgc tgaggtgctc tcgccagcac aagtcgatgt cgtcgccgtg gaaggtgatc
      781 gccgggtcga acccgcccag ggcgcggaac aggtcggcgc ggatcaacat gcaggccgag
      841 ggcagggcga acacgtcgcg cacggcatcg tgctgctcct ggtcgatctc tcccgcttcg
      901 accagcgggt cgacctcgcc gaagcggtcg acaccgagac cgacgtgttg cagcatgcgg
      961 tggtcgtccc acgtcaccag cttgggaccg acgatgccgg cgttcgaacg gtacagctcc
     1021 tcgaccagga tgcgcgttgc cgtcggttcg agcacgacgt cgtcgtgcat catcaagaaa
     1081 tannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngc acctcgttgg cggccggccc
     1141 gtagccgggg ttgccaccga ccgggcgcac gaacgcgccc ggcaccttgg cgcggatggt
     1201 ggcggccaga tcgcccggct caccggcgat gaggaacagc gtcttgaggt tgggatagtc
     1261 ctgcgctgcc agcgcacgga gcaactcgtc gaagtgagca tcgaagcgca cggccgtgtg
     1321 gtcggtgggc cggtggacca ccatgaccgc cacgacggac agcgcgggtt cggctttggc
     1381 atccacgagc aagcgaggct agcgggccac cccnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt
     1501 gcaacgcggt gatctcgaac tggcggctgc gggcgtcggc gagcacgttc tggaacgcga
     1561 cgaggctggc ccggtaggtg acgagtgcgg cctgcatgct gggatcggtc ttggcggcct
     1621 gcaactgctg acccgctgtg tcgagatcgt gcgcggccgc ctggctgtcc gtcttcgcct
     1681 tgtccgctgc tgtccgcttg ccggcgacat cgaccttggc tgccttcagc gtcgtcttcc
     1741 ctccgccgat gcgccgtgtg aacacgttga gggcatcgtc gatcgccttg aggttctgcg
     1801 tggcggcgcg caggtcgttg tcggcctgca ccttggcgcc agcgagggct gtgtcgatgc
     1861 ccttcaggcg cttccactcg gccgacagtg ctgtcaccac gtcgcggaac tcgctcaccg
     1921 cctcctgatc gtcgatcttg gcctggccca ggtcggccaa ctgcttgtcg atgtcgccag
     1981 ggatctggct gcgcaacaga cggatgcggg cctcgatcgc cgcctgcagc ctctcggcag
     2041 gtgtcgacgg gtcaccgagc aatgcatcga tcaacggatc ggtctgatgc gtggtgaacg
     2101 cgaccgggat cgagagccgc gtgtccttgc cgttggcctt gatcgtcgcg ttgtcgttcc
     2161 acagcttggc cgacgcgaga aacgcagccg acggggcacc acgcgagatc atccggaacg
     2221 gcaacggcac ggcggtggcg ctgtcggccg gtgcccggaa gccccattcg acagaaccga
     2281 ggtacgcacc ttcgacgggc ccggacacgc ccagggccgc cgtctcgaag gtgatcgcga
     2341 tctcctcacc ggccttggcc gagtcgtccc acgcccggga tcgtgtcggc tcgtcgcgca
     2401 gcgtcgcctg gtcgacctcg atgtgatatt cgccaccctc ggtcactttg aagggccacg
     2461 agaagtaggt ctcgggagat acccagcctg tcagggacga cagcgccgag ttgatcgagg
     2521 cggatcgatc tccgcgtacc cgcgcccggt tgcccttgat ctcgccgccg ggcttcaact
     2581 cgacgatgct ctgcatcgac tttgcgagcg tgttggcgaa gttcttggcc tgggcgccgt
     2641 ggatcggatg cttctcccac tccttcagca cg
//
LOCUS       DAORNF010000475         2672 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_28894_length_2672_cov_1.328238, whole genome shotgun sequence.
ACCESSION   DAORNF010000475 DAORNF010000000
VERSION     DAORNF010000475.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28894_length_2672_cov_1.328238"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>838
                     /locus_tag="PLV68_16260"
     CDS             <1..>838
                     /locus_tag="PLV68_16260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02856.1"
                     /translation="DDNNLEHDQHSASPTTSDRTAWDVIEEACTQRLTGELALTTAAG
                     GTTRVYVNAGLIYFAEHDGAPSLAEQLVIAGALDDQQLQRGMVRLNGTEHLGRLFERD
                     ASIDRDSVELAVELITEDTLTDIAEQAVITFRSTIYRHHPSGINRWFASVPTVASPLA
                     EAAPAEVVSATAKPALTLVRPVLEPAATLDTAAPDALTDLSDVDESAVIQDVSTAVSV
                     ETVESAVTAPDSPAPDRPASDTSAVERAEGIDDATTDEPVSTGLAAATVEPQPVEAHR
                     AGA"
     assembly_gap    839..896
                     /estimated_length=58
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1169..1250
                     /estimated_length=82
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1578..>1789
                     /locus_tag="PLV68_16265"
     CDS             1578..>1789
                     /locus_tag="PLV68_16265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02857.1"
                     /translation="MDIWPSWGHPADDEDPAAIDATQLVDDAAAIDDAQSSTVTQTTD
                     AADTRSTADFGASATDDVVIGSDFAPR"
     assembly_gap    1790..1928
                     /estimated_length=139
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1929..>2672
                     /locus_tag="PLV68_16270"
     CDS             <1929..>2672
                     /locus_tag="PLV68_16270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02858.1"
                     /translation="PLVARATPTAITSARPEIRFDPNGPRLPGMPEPTPEPVAAEDAS
                     VGETAAPADAPAPGAAGDDELAWVAAATRQSAPEPPVVFELTSSVPFLDDDTAPVAVT
                     PTAPAPSIQPDCSEEAVVSSTEPAMAEPAMAEPAMAEPAMAEPASDEPTLVTASAPSA
                     AEELAEVVGSSDQSVDEQAPAPKPASGLTTLGTLTPLSSLLSRPATPAADDTTARDVD
                     ASDADATPVHGTARPSTSATSPLTPLGTL"
BASE COUNT          426 a          929 c          739 g          299 t
ORIGIN      
        1 cgacgacaac aacctcgaac acgatcaaca ctcggcttcg ccaacgacca gcgaccgcac
       61 agcctgggac gtgatcgaag aggcatgcac ccagcgtctc accggcgagc tcgctctgac
      121 gactgccgcc ggaggtacga cccgcgtgta cgtcaacgcc ggactgatct acttcgccga
      181 gcacgacggt gcgccgtcgc tggccgagca attggtgatc gccggggcac tcgacgacca
      241 gcagttgcag cgcggcatgg tccgcctcaa cggcaccgaa cacctcggtc gcctgttcga
      301 acgcgacgcc tcgatcgatc gtgactcggt ggagctggcg gtcgaactga tcaccgagga
      361 caccttgacc gacatcgccg agcaagcagt gatcacgttc cgcagcacga tctaccgcca
      421 ccaccccagc ggcatcaacc gttggttcgc ctcggttccc accgtcgcca gtcctctcgc
      481 cgaagccgcc cctgcggagg tggtcagcgc gacggccaaa ccagccctga ccctggtccg
      541 ccccgtgctc gaaccagcgg ccacgctcga caccgcagcg cccgatgctc tgaccgacct
      601 cagcgatgtc gacgagagcg cggtgatcca ggacgtgtcg accgccgtca gcgtcgagac
      661 tgtcgagtct gcggtgaccg cgcccgacag tcctgcgccc gaccggcccg cgtccgacac
      721 gtccgccgtc gagcgcgccg agggcatcga cgacgcgacg accgacgagc ccgtgtccac
      781 tgggctcgcc gcggccaccg tggaaccgca gccggtcgag gcgcaccggg ccggtgcgnn
      841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnagcc
      901 gcactcgacg cgaccgaatc gcaatcacac gacgatgcct cgctcggcgc caccggctgg
      961 gacagtctga tcgcagccgt cgagcctgca cccgtcgagc ccgcacgcgt ggagcccgca
     1021 gccgtcgagc ccgagttcga ctgggacgcc cttcccgtcg agcccgcgtg ggcaacccgc
     1081 accgccgagg agaccgattc ggacagcgat tcggacagcg attcaaacag tgactcggcg
     1141 cccgcggctt gcaccttcgt gtcgcccgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gccgacgccg
     1261 acgccgacag catcgacatg ccggcaagca cgccaacgtc cgaacacgtc cctgtcgctg
     1321 ggcttgacgc caccgcgatc gaagcagacg tctggccgtc ctgggatcag gcgaccgacg
     1381 gtgacctcac gaacgcggac gtcgcgacag aagcgccgac gcagagcgaa gtcgccgctg
     1441 cgcaagtcga caccgacgcg ccggcattct gggactcggt catcaccgac agcgatctcg
     1501 gtgccgacca ggatgccgac gagccgactt gggagcacat cgtcgctgag gcaacagcca
     1561 gcaccggcac caccgagatg gacatctggc catcgtgggg acacccggcc gacgatgaag
     1621 atcccgcagc catcgatgcc acacagttgg ttgatgatgc cgcagccatc gatgacgcac
     1681 agtcgtcgac agtgacgcag acgaccgacg cggcggacac caggagcacc gccgactttg
     1741 gcgcctccgc gacagacgac gtggtgatcg gctccgactt cgcacctcgn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnag ccgctcgttg cccgggcgac accgaccgcg atcaccagcg cccgccccga
     1981 gatccgcttc gatcccaacg gcccccgtct gccgggcatg cccgaaccca caccggagcc
     2041 ggtcgcggcc gaggatgctt cggtcggcga gacggcggcg cccgccgacg cgcccgcgcc
     2101 gggcgcggct ggcgacgacg aactggcctg ggttgcggca gcgacccgcc aatcagcacc
     2161 tgagccgccc gtggtcttcg agctgacctc gtcggtgccc ttcctcgatg acgacaccgc
     2221 cccggtcgcc gtcacgccca ccgccccggc tccgagcatc cagccagact gctccgaaga
     2281 ggccgtggtc tcctcgaccg aaccagcgat ggccgaaccg gcgatggccg aaccggcgat
     2341 ggccgaaccg gcgatggccg aaccagccag cgacgaacca accctggtca ccgcatccgc
     2401 gccctcggcc gccgaggagt tggccgaggt cgtcggctcg tccgaccaga gcgttgacga
     2461 acaggcgccc gctcccaagc cagcctccgg gctgaccacg ttgggcaccc tgacccccct
     2521 cagctccctg ctctcgcgtc ccgcaacgcc ggccgccgac gacacgaccg cacgcgacgt
     2581 cgacgcgagc gacgccgacg ccacgccggt tcacggcacg gctcgcccgt ccacgtccgc
     2641 cacctcgccg ctgaccccat tgggcaccct gg
//
LOCUS       DAORNF010000476         2666 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_28969_length_2666_cov_1.400996, whole genome shotgun sequence.
ACCESSION   DAORNF010000476 DAORNF010000000
VERSION     DAORNF010000476.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2666
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28969_length_2666_cov_1.400996"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>225)
                     /locus_tag="PLV68_16275"
     CDS             complement(<1..>225)
                     /locus_tag="PLV68_16275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02859.1"
                     /translation="VLVRVPNINFRWTTVGADSDLVGQVPNPPGMQAALSDVTVATGE
                     SDPVNGGITPIPRLNLTYFDGLDSIPGTQR"
     assembly_gap    226..494
                     /estimated_length=269
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<495..725)
                     /locus_tag="PLV68_16280"
     CDS             complement(<495..725)
                     /locus_tag="PLV68_16280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02860.1"
                     /translation="MTSLPLGAAAAASPGAIAAESQAWALPTIEIGDGSDTPGTGDGI
                     GAARVEAPQPDAPVAFLDVPTDPAAGVHGMVWL"
     gene            complement(881..1390)
                     /locus_tag="PLV68_16285"
     CDS             complement(881..1390)
                     /locus_tag="PLV68_16285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02861.1"
                     /translation="MALPINSATRDFPMTPGAGGASTATITGVVSAGAAQRISLTIEP
                     SLRLLLSSDSPPAFVTSGDVLTWTWSISATGGATNQARLTHTISGAELDVTAASMPPG
                     CALTGAVGRWAIDCAISARLTESPVTIVASVSVQSVAGGETVETLTVLGEPSGWSMGA
                     QSSSAIQTV"
     gene            complement(1359..>1929)
                     /locus_tag="PLV68_16290"
     CDS             complement(1359..>1929)
                     /locus_tag="PLV68_16290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02862.1"
                     /translation="TAILPFAAGASNGGRKLLAWLLRPRVAAALVAAVVTAGAVTWMI
                     PHGDAPVAAPVITTVTTIPVANTSSPPPPASTTSAVIASPIAATTTAVPTTTTALIVP
                     PPPPPPDPTPIETAVLTTVAVPTTGEVVGTVMSPAGGTVGWLPIVLTDASGVVYRTTS
                     DAAGAFRLASLAPGALHRHRGPADQLRHP"
     assembly_gap    1930..1939
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1940..2550)
                     /locus_tag="PLV68_16295"
     CDS             complement(<1940..2550)
                     /locus_tag="PLV68_16295"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRE02863.1"
                     /translation="MVAQIARARGIDPDDVEDVVAEVFESTLRALRNGSGPIDEIAPY
                     LNVTVQRQARRHRLRRRVDPVPAIEIEPAAIVKDDHSEVWHDEQGVIAAAFATLSDHR
                     RIILWMSAVEDQTPQAIGESLGISHDSVRAAISRSRRELRAAYFAEHSRRRGSIACAS
                     FRSELLTGRDVSVAASAHVATCVACSNAIRGVNSVESSLRGLGV"
BASE COUNT          405 a          811 c          810 g          361 t
ORIGIN      
        1 caccgttggg taccggggat gctgtcgagt ccgtcgaagt acgtcaggtt gagccgtggg
       61 atcggagtga tcccgccgtt gaccgggtcg gactcacccg ttgccaccgt gacgtcggag
      121 agggcagcct gcatgcctgg cgggttcggc acctgcccga ccaggtcgga gtcggcgccc
      181 accgtggtcc accggaagtt gatgttcggc acgcggacca gcacgnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      481 nnnnnnnnnn nnnngagcca caccatgccg tgcacacccg ccgccgggtc ggtgggcacg
      541 tccaagaacg cgaccggagc atcgggctgc ggtgcttcga cacgtgccgc accgatgccg
      601 tcaccagttc ccggtgtgtc ggagccgtcg ccgatctcga tggtgggcaa cgcccatgcc
      661 tgcgattccg cggcgatggc acccggtgat gccgccgccg cggctccgag tggaagcgat
      721 gtcacggcca cgcaaagcgc cgccaacacc gccggtcgga cgcgactacg cgcgccagta
      781 ggttcccgcc tgttgttcat gccgccctct aagtaccctc ctgaccaatc tgtgaccgag
      841 tgtgatggac gtcacaacgg tcgtcgacca ccgaacccgg tcagaccgtt tggatggccg
      901 acgacgactg agcgcccatc gaccaacccg atggttcgcc gagcacggtc aacgtctcca
      961 ccgtctcgcc accggccacc gactggaccg acaccgacgc aacgatggtg accggggatt
     1021 ccgtcaggcg ggccgagatc gcacagtcga tggcccaacg acccacggct cctgtcagcg
     1081 cgcatccagg cggcatcgat gctgccgtga catcgagctc ggcaccgctg atcgtgtggg
     1141 tcagtcgggc ctggttggtg gcaccacccg tcgccgagat cgaccacgtc cacgtcaaca
     1201 cgtcacccga ggtgacgaag gcgggcggac tgtcgctcga cagcagcagg cgcaggctgg
     1261 gctcgatcgt cagcgagatt cgctgcgccg cacccgcgct gaccacgccg gtgatcgtcg
     1321 ccgtcgacgc gcccccggca ccgggtgtca tcgggaagtc acgggtggcg gagttgatcg
     1381 gcagggccac ggtggcggtg tagggccccg ggcgcaagcg acgcaagacg aaacgcaccg
     1441 gccgcatccg acgtcgtgcg gtagaccaca ccgctggcgt cggtgaggac aatcggcaac
     1501 cagcccacgg tgcccccggc gggggacatc accgtgccga cgacctcgcc ggtcgtcggc
     1561 acggcgaccg tggtcaggac ggcggtctcg atcggcgtcg ggtccggcgg tggcggtggc
     1621 ggtggcacga tcagggctgt cgtcgtggtc gggaccgcgg tcgtggtggc ggcgatcggc
     1681 gatgcgatga cggcgctggt cgtgctcgcc ggaggtggcg gtgatgacgt gttcgcgact
     1741 gggatggtgg tcaccgtggt gatcaccggt gcggcgaccg gagcatcacc gtgcgggatc
     1801 atccatgtca ccgcgccggc cgtgacgacc gctgcaacga gtgccgccgc aacccggggc
     1861 cgcagcagcc aggcaagcaa cttgcgccca ccgttggatg cgccggcagc gaacggcaga
     1921 atcgccgtgn nnnnnnnnna caccgagacc acgcagcgac gactcgacgc tgttgacccc
     1981 gcggatggcg tttgagcatg ccacgcatgt cgccacgtgg gcactcgccg caacactcac
     2041 atctcgtcca gtgagtagtt ctgaacggaa cgaagcgcag gcgatgctcc cgcggcggcg
     2101 gctgtgttcg gcgaaatatg cggcacggag ctcgcgacgc gaccgcgaga tggcggcccg
     2161 caccgagtcg tgcgagatgc ccagcgactc gccaattgcc tgcggcgtct ggtcctccac
     2221 agcggacatc cacaagatga tccggcgatg atcgctgagg gtggcgaatg cggcagcgat
     2281 cacaccctgc tcgtcgtgcc acacctcgga atgatcgtcc ttgacgattg ccgcgggttc
     2341 gatctcgatc gccggcaccg gatcaacgcg ccggcgcagg cgatggcggc gagcctggcg
     2401 ctgcaccgtc acgttgaggt acggcgcgat ctcgtcgatc gggccactgc cgttgcgcaa
     2461 ggcacgcagc gtggattcga acacctcggc gaccacatcc tcgacgtcgt cgggatcgat
     2521 cccacgagcc ctcgcgatct gcgccaccat ctcgcgatgg cgcagataca gctcggcaaa
     2581 cgcagcgtca cttccgctgc ggcacagcgc caagagcgct gcatcgcttt caggttccgc
     2641 cagctgtcca cccgtagaca atgaca
//
LOCUS       DAORNF010000477         2662 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_29023_length_2662_cov_2.046030, whole genome shotgun sequence.
ACCESSION   DAORNF010000477 DAORNF010000000
VERSION     DAORNF010000477.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2662
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29023_length_2662_cov_2.046030"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2293
                     /locus_tag="PLV68_16300"
     CDS             <1..>2293
                     /locus_tag="PLV68_16300"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02864.1"
                     /translation="GSQQAQAIDGTSFTAEPGAAYNCAITNTKLGQITVVKNVDGADG
                     TFDFNGTFASPNGFQLTTVGGTANQLFNSIPAGTYSLSELELPSDYDGVLLCSETGTK
                     DTTVNGLVGTINLQAGESVTCTFTNTQRSTILVDKVTNPSGDATEFDFTLGATPFTLT
                     DAAAPFSSGLVVPGTYTVTELAETGWTLTGLSCPTGVRGSSASIVGSAAEITLTAGDT
                     ITCTYTNAKLGSVTVDKDASAITHVPVDGFPNRYQVDYTVVATNGAYVPTTYDAVDAL
                     SFGGNTTIVSATVLSKPDDVTLVAGWNGQGNTLLADDAAIAARGTHTYTIRVVFDVAP
                     AITPSERDCRLGTDENGTGTLNTVTVTAPNGGDDDDACTPIPDPNVKVAKSVSSAPLR
                     NADGTWTIGYDVTVTNTTDDTHTIVGPGTYVANDTLAFGGDVTVVSVTASAVTSGLAA
                     NVNDDFDGAASGDTELAAGSIAAGATHVYRVTVVADVVPTTTGGGECDASFTSGRGFV
                     NRATVTVDDVTTPPSSACAPYATLTLNKVLHNDDGGVATLSDFVLSATPTTPDGAAAI
                     INGADPDGTKNAAPADGVTAAVPVGSYTLAETEQSGYTAGNWVCTGGTVTNDVVVVTA
                     GADVICTIVNDDDPVDLQLTKDDGGAVAVAGGPAYDYTITVVNVGTRNPDLGEPVTVT
                     DILPAGLSFVTVPSNCTTSGQIATCDIDPALLKVGDDPVVITISVKLAAGAPAGTYVN
                     KAFVTTEDDPACVGDHCVPECPPT"
     assembly_gap    2294..2303
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2304..>2662
                     /locus_tag="PLV68_16305"
     CDS             <2304..>2662
                     /locus_tag="PLV68_16305"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02865.1"
                     /translation="VKGPLAESGTDASNNVDCEPTPGIRNATITVTKTDSVDQPIHPG
                     DTFSYAIVVTNNGPSTVTNVSIDDDLPNELTLVSATGVDWTCGTSDPLACTYAKTLEP
                     NNAAPAITVVVRLAATYR"
BASE COUNT          484 a          987 c          823 g          358 t
ORIGIN      
        1 ctggatcgca gcaggcccag gcgatcgacg ggacgagctt caccgccgaa cccggtgcgg
       61 cctacaactg cgcgatcacc aacaccaagc tcggccagat cacggtggtc aagaacgtcg
      121 acggcgccga cggcaccttc gacttcaacg gcacgttcgc ctcgccgaac gggttccagc
      181 tcacgacggt cggcggtacg gccaaccagc tgttcaacag cattccggcc ggcacctact
      241 cgctgtccga gttggaactg cccagcgact acgacggcgt gctgctgtgc tcggagaccg
      301 gaaccaagga cacgaccgtg aacggtctgg tcggcacgat caacctgcag gctggcgagt
      361 cggtgacctg cacgttcacc aacacgcagc gctcgaccat cctggtcgac aaggtcacca
      421 accccagcgg tgacgcgacc gagttcgact tcacgctcgg cgccacgccg ttcaccctga
      481 cggacgcggc cgcgccgttc agctcgggcc ttgtcgtgcc cggcacctac acggtgaccg
      541 aactggccga gaccggctgg accctgaccg gtctgtcgtg ccccaccggg gtgcggggca
      601 gttcggccag catcgtcggg tccgcggccg agatcaccct cacagctggc gacacgatca
      661 cctgcaccta caccaacgcc aagctcggct cggtcacggt ggacaaggat gcgtcggcga
      721 tcacccacgt ccccgtcgac ggcttcccca accgctacca ggtcgactac accgtcgtgg
      781 ccaccaacgg ggcctacgtg ccgaccacgt acgacgcggt cgacgcgctg agcttcggtg
      841 gcaacaccac gatcgtgtcg gccaccgtgc tgagcaagcc cgacgacgtc acgctcgtcg
      901 ccggctggaa cggccagggc aacacgctgc tggccgacga tgcagcgatc gcggctcgtg
      961 gcacgcacac ctacacgatc cgggtcgtgt tcgacgtcgc tccggcgatc acgcccagcg
     1021 agcgggattg caggctcggc accgacgaga acggtacggg cacgctcaac accgtgaccg
     1081 tcaccgcccc caacggcggt gacgacgacg acgcctgcac gccgatcccc gacccgaacg
     1141 tgaaggtggc caagtcggtc agcagtgccc cgctgcgcaa cgccgacggc acctggacca
     1201 tcggttacga cgtcaccgtc accaacacga ccgacgacac ccacaccatc gtcgggcctg
     1261 gcacctacgt cgccaacgac acgctcgcct tcggcggtga cgtcaccgtc gtcagcgtca
     1321 cggcgtcggc ggtcaccagc ggcctcgccg ccaacgtcaa cgacgacttc gacggcgccg
     1381 caagcggcga caccgagctc gctgccggtt cgatcgccgc gggtgccacc cacgtctacc
     1441 gggtcaccgt cgtcgccgac gtggtgccga ccacgaccgg cggcggtgaa tgcgacgcca
     1501 gcttcacgtc gggccgcggc ttcgtcaacc gggcgacggt caccgtcgac gacgtgacca
     1561 caccgccgtc gtcggcctgc gctccgtacg cgacgctgac gctgaacaag gtgctgcaca
     1621 acgacgacgg tggtgtggcc acgctgagcg acttcgtgct cagcgccacg ccgacgacgc
     1681 ccgacggtgc ggccgcgatc atcaacggtg ccgaccctga cggcaccaag aacgcggcac
     1741 ctgccgacgg cgtcaccgcc gccgtgccgg tcggcagcta cacgctggcc gagaccgagc
     1801 agtccggcta caccgctggc aactgggtct gcaccggtgg cacggtcacc aacgacgtgg
     1861 tcgtcgtgac ggccggcgcc gacgtgatct gcaccatcgt caacgacgac gatccggtcg
     1921 atctgcagct gaccaaggac gacggcggcg cggttgccgt cgccggtgga ccggcgtacg
     1981 actacacgat cacggtcgtc aacgtcggca cccgcaaccc cgacctgggc gagccggtga
     2041 cggtcaccga catcctcccc gccgggctca gcttcgtgac ggtgccgtcg aactgcacca
     2101 ccagcggcca gatcgccacg tgtgacatcg acccggcgct gctcaaggtc ggcgacgacc
     2161 ccgtggtgat caccatctcg gtcaagctgg ccgcgggtgc gccggcgggc acgtacgtca
     2221 acaaggcgtt cgtgaccacc gaggacgacc cggcctgcgt gggcgaccac tgcgtgccgg
     2281 agtgcccacc cacnnnnnnn nnngtgaagg gaccactggc cgagtcgggc acggatgcgt
     2341 cgaacaacgt cgactgcgag ccgacacccg gcatccgcaa cgcgacgatc acggtcacca
     2401 agaccgattc ggtcgaccag ccgatccacc ccggcgacac cttcagctac gccatcgtcg
     2461 tcaccaacaa cggcccgtcg accgtcacca acgtgtcgat cgacgacgat ctgccgaacg
     2521 agttgacgct ggtgtcggcc acgggcgtcg actggacctg cggcacctca gacccattgg
     2581 cgtgcaccta cgccaagacg ttggagccga acaacgccgc tccggccatc acggtcgtgg
     2641 tgcgcctggc ggcgacctat cg
//
LOCUS       DAORNF010000478         2655 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_29123_length_2655_cov_1.415000, whole genome shotgun sequence.
ACCESSION   DAORNF010000478 DAORNF010000000
VERSION     DAORNF010000478.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29123_length_2655_cov_1.415000"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="PLV68_16310"
     CDS             complement(<1..233)
                     /locus_tag="PLV68_16310"
                     /inference="COORDINATES: protein motif:HMM:NF013425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HRE02866.1"
                     /translation="MTGAAHLAASAALRAGAGMVHLSSPGVIPSGEHEYVHRSVPAFG
                     WSTDVLVGLDRFQSLVIGPGLGRGDDMVLSIRDV"
     assembly_gap    264..378
                     /estimated_length=115
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<379..>653)
                     /locus_tag="PLV68_16315"
     CDS             complement(<379..>653)
                     /locus_tag="PLV68_16315"
                     /inference="COORDINATES: protein motif:HMM:NF015791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HRE02867.1"
                     /translation="GAQVRVVDAADAPARIERADLVIDAAYGTGFRGEWVAPSVGDGV
                     AVLAVDIPSGVDGLTGRAGVGVLAADATVVLAALKPGVLFGAGAALAG"
     assembly_gap    654..791
                     /estimated_length=138
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1134..1499)
                     /locus_tag="PLV68_16320"
     CDS             complement(1134..1499)
                     /locus_tag="PLV68_16320"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HRE02868.1"
                     /translation="MIGIGVDAVEIERFRRSLARTPSMRTRLFTAIELDYVAPKADPV
                     PSLAARFAAREALMKSLGVGLGAFGFHDAWVERADSGVPTLALTGAAAALAADRGVRR
                     WHLSLTHSDTLAIAYVIAE"
     assembly_gap    1634..1782
                     /estimated_length=149
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1783..>2655)
                     /locus_tag="PLV68_16325"
     CDS             complement(<1783..>2655)
                     /locus_tag="PLV68_16325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucosamine-6-phosphate synthase"
                     /protein_id="HRE02869.1"
                     /translation="KEVAIFRAHKALPIVVATEGETRFAAAAHVVAVPGVDPALAFVL
                     SAMAGHLFGYEAARAIDATALPLREMREVIERALMSTDVGDEVMRLVRPELDPIAERF
                     FAALRTGVYDGQLEASTAVRVSSMLRDALGPAPLEAYQAATGNIATPGMLIDDLTAAL
                     TRAIEELTRPIDAIKHQAKTVTVGISRNDEGVLDRALVQQVLAAGVGRDRLTYKSLKV
                     LADLDPAVADVVGFTRYHIEGEPQSGMATIAVADRGGISREVPSRVDRNPSLLGTKRR
                     VAMEREVLVARGRS"
BASE COUNT          367 a          837 c          755 g          294 t
ORIGIN      
        1 acatcgcgga tcgacagcac catgtcgtcg ccacgaccca agccggggcc gatcaccagc
       61 gactggaacc ggtcgaggcc gacgagcacg tcggtgctcc acccgaaggc cggcacggac
      121 cgatggacgt actcgtgctc gccggagggg atgacgcccg gcgacgacag gtggaccatg
      181 cctgcgccag cacgcagcgc ggccgacgcg gcaaggtggg ccgccccggt catgccggga
      241 ctgcccgcaa ccacacgggt tgcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnncc cgccagtgct gccccggcgc cgaacagcac acccggcttc
      421 agcgccgcga gcaccacggt ggcgtcggcg gcgagcacgc ccacacccgc gcggccggtc
      481 agcccgtcga cacccgaggg aatgtcgacc gccaacaccg ccacgccgtc gccgacgctc
      541 ggagccaccc actcaccgcg gaaaccggtg ccgtacgcgg cgtcgatcac cagatcggcc
      601 cgctcgatcc gcgccggagc atccgccgca tcgacgacac gcacctgcgc gccnnnnnnn
      661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn ncgacatttg aagatccccg gcgaaatcag gaggtcaggc ggcgtcgtcg
      841 ccgccgcatc gatcgcccgc atctcctgag gcgtcacgat cgccaacacg cccgccaacg
      901 tacacccgcc gtgtggacgg ttgggtggca ggtatcgccc accgcgcggc tcgtctccgt
      961 gccacgcaca cagcgatcac gttgccagac gccctggtga tcgtctctgc cgcggcgcga
     1021 gcggcggatg agttgatcac aaccgaccac cgctggccga ccgcgggccg acttggtctc
     1081 cacgcgacga tcacgcggct ctgacggccg caacgcacat cgctggcggt gggttattcg
     1141 gcgatgacgt aggcgatcgc caacgtgtcg ctgtgggtga gcgagaggtg ccaacggcgg
     1201 acaccgcgat cggcggccag cgcggcggcg gcaccggtca gcgccaacgt ggggacgcca
     1261 gaatcggctc gttcgaccca cgcgtcgtgg aagccgaacg cgccgaggcc cacgcccagc
     1321 gacttcatca acgcctcccg cgccgcgaag cgagcggcca gcgacggcac cgggtcggcc
     1381 ttgggcgcga cgtagtccaa ctcgatcgcg gtgaacaacc gcgtccgcat cgacggcgta
     1441 cgcgccaggc tgcgccggaa ccgttcgatc tcgaccgcgt cgacgccgat gccgatcaca
     1501 ctggcctcac gatcactggc ctcacgcgcg ccagtggtcg gcggtctcgc tgatcggcag
     1561 gatcagcgcg tcggccaccg acaggtcggc caggcgatcg tcgcggaagc tgccctcggt
     1621 ctctgtcacc cagnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncgctgcgg ccacgggcga
     1801 cgagtacctc gcgctccatc gcgacccgcc gcttggtgcc gagcaggctc gggttgcgat
     1861 cgacgcgcga cgggacctcg cgcgagatgc ccccacgatc ggcgaccgcg atcgtggcca
     1921 tgcccgactg cggctcgccc tcgatgtggt agcgggtgaa gccgaccacg tcggccacag
     1981 ccggatcgag atcggccaac accttgagcg acttgtaggt gagccggtcg cggccaacac
     2041 ccgcggcgag cacctgctgc accaacgccc ggtcgagcac cccttcgtcg ttgcggctga
     2101 tgccgacggt gaccgtcttg gcctgatgct tgatcgcgtc gatcggccgg gtcagttctt
     2161 cgatcgcgcg ggtgagcgcg gcggtcaggt cgtcgatcaa catgccgggg gtggcgatgt
     2221 tgccggtcgc ggcttggtac gcctccaacg gagcgggacc gagcgcgtca cgcagcatcg
     2281 acgacacgcg caccgcggtc gatgcttcca actggccgtc gtagacgccc gtgcgcagcg
     2341 cagcgaagaa ccgctcggcg atcgggtcga gctcgggccg caccaggcgc atcacctcgt
     2401 cgccgacgtc ggtcgacatc agcgcccgct cgatcacctc gcgcatctca cgcagcggca
     2461 gggccgtcgc gtcgatcgcc cgtgccgcct cgtagccgaa caggtgcccc gccatcgccg
     2521 acagcacgaa ggccaacgcg gggtcgacac cgggtacggc caccacgtgt gccgcggcgg
     2581 cgaagcgggt ctctccctcg gtggcgacca cgatcggcaa cgccttgtgg gcgcggaaga
     2641 tcgcgacctc cttgg
//
LOCUS       DAORNF010000479         2643 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_29290_length_2643_cov_1.226043, whole genome shotgun sequence.
ACCESSION   DAORNF010000479 DAORNF010000000
VERSION     DAORNF010000479.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2643
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29290_length_2643_cov_1.226043"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="PLV68_16330"
     CDS             complement(<1..283)
                     /locus_tag="PLV68_16330"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRE02870.1"
                     /translation="MSADAFAVVTGGGTSGHVLPALAIAEGLVAGGHRADEIAYIGAT
                     RGIETRLVAATPYQRYFLDVVGLQRRLTAKNLVFPFKLVRSIWEARRLLR"
     gene            complement(273..>677)
                     /locus_tag="PLV68_16335"
     CDS             complement(273..>677)
                     /locus_tag="PLV68_16335"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HRE02871.1"
                     /translation="QAMIGMATGGVGGTGVGRGPSKWGYLPVADSDFIFAVIGEEMGL
                     IGVVAVIGAFMLLTYAGVQIALAARDRFGTLLAGGIVAWMAVQATINIGGVSGAMPVT
                     GLTLPFVSSGGSSLFVSMAAIGLLLNVARHVR"
     assembly_gap    678..825
                     /estimated_length=148
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<826..1462)
                     /locus_tag="PLV68_16340"
     CDS             complement(<826..1462)
                     /locus_tag="PLV68_16340"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HRE02872.1"
                     /translation="MTTVASVGERRRLAIERADARRGHSIQRALRPTKPPPTAFYLVI
                     VVVTVLIMLGLVMVFSASSITALHRNQSPWRIFNRQLLWAILGLGALVMAARIHYHRW
                     RRWSSMLLLGAYGLMLLPFVPGIGTSVNGARAWVYIGSFGFQPSEFLKLALLIFCADL
                     LAKRETEMADLGRTLKPMMVVALLGAGMCLAQGDLGSAIVIAAIVFVVAFLA"
     gene            complement(1471..>1714)
                     /locus_tag="PLV68_16345"
     CDS             complement(1471..>1714)
                     /locus_tag="PLV68_16345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02873.1"
                     /translation="GVVAIGDAAELIESIFAPVCTVVRATSMADAVSAAAGLARSGDA
                     VVLSPACTSFDWYSGYPARGDDFRTLVQQHLEDSGR"
     assembly_gap    1715..1846
                     /estimated_length=132
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1847..>2404)
                     /locus_tag="PLV68_16350"
     CDS             complement(<1847..>2404)
                     /locus_tag="PLV68_16350"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HRE02874.1"
                     /translation="VAVGNTEVPLVEVIDDEAIDAFVVECSSFRLNWTTCFRPDAAVW
                     LNLAPDHLNWHTGLDSYVAAKTRMWRHQRSDDTAIGLADDAVVMAALERVNGRAISFG
                     FDRGDYRSVAAGDHRELVGPAGPLTTTAAMRRALPHDVTNALAAAALVQQAGLAGAEA
                     IAAALADFVGPPHRIEPVGSKDGVA"
     assembly_gap    2405..2436
                     /estimated_length=32
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2437..>2643)
                     /locus_tag="PLV68_16355"
     CDS             complement(<2437..>2643)
                     /locus_tag="PLV68_16355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02875.1"
                     /translation="MVRWSDIVVPAPAVPETHPVVVAALAWHRPLRTEIDLAAEWEAQ
                     RGRDARPMLAVTGTDGKTTTTLMAT"
BASE COUNT          402 a          823 c          798 g          308 t
ORIGIN      
        1 tgcgcagcaa tcggcgtgcc tcccagatcg accggacgag cttgaacggg aacaccaggt
       61 tcttggccgt caggcgccgt tgcagcccga ccacgtcgag gaagtagcgc tggtacgggg
      121 tcgcggcgac cagtcgggtc tcgatgccgc gggtggcgcc gatgtaggcg atctcgtcgg
      181 cccggtgacc gcccgcgacc agaccttcgg cgatcgccag cgcgggcagc acgtggcccg
      241 aggtgccacc accggtgacg acggcgaacg cgtcagcgga catggcgggc gacgttgagc
      301 aacaggccga tcgccgccat cgagacgaac agcgagctgc cgcccgagga cacgaacggc
      361 aacgtcaggc cggtgaccgg catcgccccg ctgacaccgc cgatgttgat cgtcgcctgc
      421 accgccatcc acgccacgat gcctccggcc agcaacgtgc cgaacctgtc gcgagcggcc
      481 agggcgatct gcacgccggc ataggtcagc agcatgaacg caccgatcac cgcgacgacg
      541 ccgatcagac ccatctcctc accgatcacg gcgaagatga agtcgctgtc ggctacgggc
      601 aggtagcccc acttcgacgg accgcgcccg acacccgtgc cgccgacgcc accggtcgcc
      661 atgccgatca tcgcctgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncggcc aggaacgcga
      841 cgacgaacac gatcgccgcg atcacgatcg ccgaaccgag gtcgccttgc gccaggcaca
      901 ttccggcccc gagcagggcg acgaccatca tcggcttgag cgtgcggccc agatcggcca
      961 tctcggtctc gcgcttggcg agcaggtcgg cgcagaagat cagcagggcc agcttcaaga
     1021 actcggacgg ttggaagccg aacgaaccga tgtacaccca tgcccgggca ccgttgaccg
     1081 acgtgccgat gcccggcacg aacggcaaca gcatcaggcc gtacgcgccg agcagcagca
     1141 tcgagctcca ccgccgccag cggtggtagt ggatgcgcgc ggccatcacc agcgcgccga
     1201 ggcccaggat cgcccacagc agttggcggt tgaagatgcg ccacggtgac tggttgcggt
     1261 gcagggcggt gatcgacgat gccgagaaca ccatcaccaa gccgagcatg atcagcaccg
     1321 tgaccaccac gatcaccaga tagaaggcgg tcggcggtgg cttggtcgga cgcagggcac
     1381 gctggatcga gtggccgcgg cgggcgtcgg cccgttcgat cgcgaggcgg cggcgctcac
     1441 cgaccgacgc tacagtcgtc atcacactgg tcatctgcct gaatcctcca ggtgttgctg
     1501 aaccaaggtc cggaagtcgt cgccgcgggc cggatacccg ctgtaccagt cgaagctggt
     1561 gcacgccggc gacagcacca cggcatctcc ggaccgagcc aggcccgcgg cggcgctcac
     1621 ggcgtcggcc atcgacgtgg cgcgcaccac cgtgcacacc ggcgcgaaga tcgactcgat
     1681 cagctcggcc gcgtcaccga tcgcgacgac gccgnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncagg cgaccccgtc
     1861 cttgctcccg accggctcga tccggtgcgg tggtccgacg aagtcggcca gcgcggcggc
     1921 gatcgcctcg gctccggcca ggccggcctg ctgcaccaac gctgcggcag cgagcgcgtt
     1981 ggtgacgtca tggggcaggg cgcggcgcat cgctgcggtc gtcgtcagcg gaccagccgg
     2041 cccgacgagc tcgcggtgat cgccagccgc gaccgagcgg tagtcaccgc ggtcgaagcc
     2101 gaagctgatc gcccggccgt tgacgcgctc gagcgcggcc atcaccacgg cgtcgtcggc
     2161 gaggccgatc gcggtgtcgt cggaacgctg atggcgccac atccgggtct tggccgcgac
     2221 gtagctgtcg agaccggtgt gccagttgag gtgatcgggc gccaggttga gccacacggc
     2281 tgcatcaggc cggaagcacg tcgtccagtt gagccggaag ctgctgcact cgaccacgaa
     2341 cgcgtcgatc gcctcgtcgt cgatcacttc gaccagcggt acctcggtgt taccgaccgc
     2401 gacgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngtgg ccatcagcgt ggtggtcgtc
     2461 ttgccgtcgg tgccggtgac cgccagcatc gggcgcgcgt cgcggccacg ctgggcctcc
     2521 cactcggcgg cgaggtcgat ctccgtgcgc agcggccgat gccaggccag cgcggcgacg
     2581 acgaccgggt gcgtctcggg caccgccggt gccgggacga cgatgtccga ccagcggacc
     2641 atc
//
LOCUS       DAORNF010000480         2637 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_29366_length_2637_cov_1.692874, whole genome shotgun sequence.
ACCESSION   DAORNF010000480 DAORNF010000000
VERSION     DAORNF010000480.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2637
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29366_length_2637_cov_1.692874"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..631)
                     /locus_tag="PLV68_16360"
     CDS             complement(<1..631)
                     /locus_tag="PLV68_16360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D 1"
                     /protein_id="HRE02876.1"
                     /translation="MTLESMLADRQKLAYIANQAADARLNVELETEGMTLNIGPQHPA
                     THGTLRIVARLDGEQVVWAEPSAGYMHRGYEKLTEVRTFPQVTALINRIDWLGSFANE
                     VPFILAAEKLMDVEAPPRAQHIRTILFELSRIANISLFLGDLGVQLGAVTPAFYAFRD
                     REYVLNLIEAATGGRFHPNFDRIGGLKDDLPKGWIDETRQVMKKLRAYCD"
     gene            complement(628..>1148)
                     /locus_tag="PLV68_16365"
     CDS             complement(628..>1148)
                     /locus_tag="PLV68_16365"
                     /inference="COORDINATES: protein motif:HMM:NF012549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HRE02877.1"
                     /translation="VSAERWRDAGLVLRDTLGMDHFGFLSAIDWMPSPYGRGEDEPGQ
                     EVERPTEIVQGYAGGETRFQVFARVSSTTLHYGITIKADVGDDMTVPSWIPVYAGANW
                     HERECHEMFGISFAEHPNLINMYLPTGFEGYPLRKDFPLLSRIIKPWPGIVDVEPLPG
                     DDDDAEGEGDAS"
     assembly_gap    1149..1179
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1520..1718)
                     /gene="nuoB"
                     /locus_tag="PLV68_16370"
                     /pseudo
     CDS             complement(1520..1718)
                     /gene="nuoB"
                     /locus_tag="PLV68_16370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001187988.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
     assembly_gap    1719..1728
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1729..1949)
                     /locus_tag="PLV68_16375"
                     /pseudo
     CDS             complement(1729..1949)
                     /locus_tag="PLV68_16375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001187988.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
     gene            complement(1964..2329)
                     /locus_tag="PLV68_16380"
     CDS             complement(1964..2329)
                     /locus_tag="PLV68_16380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HRE02878.1"
                     /translation="MSEFLRDYLTVVWFAAIALILAGLLLGIAAAIRPDRPNYQKLIA
                     YESGVDPVGTGWSQSQIRYYVFALLFVVFDVEAVFIFPWATQLERYGAFGLMEMGVFV
                     LVLLLGLVYAWKKGVLRWV"
BASE COUNT          432 a          850 c          887 g          427 t
ORIGIN      
        1 cgtcgcagta ggcgcgcagc ttcttcatca cctgacgggt ctcgtcgatc cagcccttgg
       61 gcaggtcgtc cttgaggccg ccgatgcggt cgaagttggg gtggaaccgg ccaccggtcg
      121 cggcctcgat caggttgagc acgtattcgc ggtcgcggaa ggcgtagaac gcgggagtca
      181 ccgcgccgag ctgcaccccg aggtcaccga gaaacaggct gatgttggcg atgcggctga
      241 gctcgaacag gatcgtgcgg atgtgctggg cccgcggcgg cgcctcgacg tccatcagct
      301 tttcggcggc gaggatgaac ggcacctcgt tggcgaagct gcccaaccag tcgatgcggt
      361 tgatcagcgc cgtcacctgc gggaacgtgc gcacctcggt cagcttctcg tagccgcggt
      421 gcatgtagcc ggccgacggc tcggcccaca cgacctgctc gccgtcgagg cgggcgacga
      481 tgcgcagcgt gccgtgggtg gccgggtgct gggggccgat gttgagggtc atgccctcgg
      541 tctcgagctc gacgttgagg cgcgcgtcgg cggcctggtt ggcgatgtag gccagtttct
      601 ggcgatcggc gagcatggac tcgagcgtca cgacgcgtct ccttcgccct cggcgtcgtc
      661 atcgtcgccc ggcagcggct cgacgtcgac gatgcccggc cacggcttga tgatgcgcga
      721 cagcaagggg aagtccttgc gcagcggata cccctcgaaa ccggtcggca ggtacatgtt
      781 gatgaggttg gggtgctcgg cgaaggagat gccgaacatc tcgtggcact cccgctcgtg
      841 ccagttggct ccggcgtaga ccgggatcca gctcggcacg gtcatgtcgt cgccgacatc
      901 ggccttgatg gtgatgccgt agtgcagcgt ggtgctggac acccgggcga acacctggaa
      961 gcgggtctcg ccaccggcgt aaccctgcac gatctcggtc gggcgctcga cctcttgacc
     1021 cggctcgtcc tcgccccggc cgtacggcga gggcatccag tcgatcgccg acaggaaccc
     1081 gaagtgatcc atccccagcg tgtcgcgcag caccaggccc gcgtcgcgcc accgttcggc
     1141 actgacccnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng gccgcgcgca gcgcgtcgac
     1201 cgcggcgtcg cggcgagcat cgctgggcaa cgcggcgact gcgtcgtctc cggtggtggt
     1261 gtcagacgac aacgggctca cccttctggt cgtcggtctg gcctgtcacg gcatcggtgg
     1321 ccgtgtcgag ttccgagctc gcctcgatgg ccgcacccgc ttgggtggcg gtgtcggggc
     1381 gcagacggag cggcttggcg gcctcgatcg cggcagcctc ggcagccttg cgcttgcgcg
     1441 cctcgcggcg ggtctcgggc ttgggttcgc gggtcagcgg cttgaagccc tcgccgcgcc
     1501 aacgcgcacc catgtcttca cgcttgatgt gctgctgcag caacacgatg ccttcgagca
     1561 acgcctcggg ccgcggcgga cagccgggca cgtagatgtc gacgggcaag atctggtcga
     1621 cgcccttggt gaccgagtag ctgtcccagt acgggccgcc gcagttggcg caggacccca
     1681 tcgagatcac gtacttggga tcgggcatct gctcgtacnn nnnnnnnnat cgccggggcc
     1741 atcttgtcgg tgaccgtacc ggagatgacc acgaagtcgg cctggcgcgg gctggccggc
     1801 agcgggatga cgcccaggcg catcacgtcg taacgaggcg aggcgaaggc cgcacccatc
     1861 tcgatcgcgc agcacgcgag accccattgg tacacccaca tgctgtacga gcgaccgatg
     1921 ttgaacagct gggtcagtgg cttgggcaac cgccccttgt cgactagacc caccgcaaca
     1981 cacctttctt ccaggcgtac acgaggccga gcaacagcac cagcacgaac acgcccatct
     2041 ccatcaaacc gaaggcgccg tagcgctcga gctgggtcgc ccacgggaag atgaacacgg
     2101 cttcgacatc gaacacgacg aacagcagcg cgaacacgta gtaccggatc tggctctgcg
     2161 accagccggt gcccaccggg tcgacaccgc tctcgtaggc gatcagcttc tggtagttgg
     2221 gccggtcggg ccggatcgcc gccgcgatgc ccaacagcaa gcccgccaag atcaacgcga
     2281 tcgcggcgaa ccagacgacg gtgaggtaat cgcgaagaaa ctcggacatc ggggctagac
     2341 gctactacgg cgcgcgtttc aggccacaaa ccggggcgat cgaccagtct gacaccgctg
     2401 gtccttcggt acaggtcggt gatagtaaag cagaccttac atctgctgtc aatggccggg
     2461 taacacacat cggcaatcgc ggtgtgttat gccatcttca tgggcgacaa cattggcgcc
     2521 ggcgctggga gcggtgccgg catcggcacc ggcgtcggcg gcacggctgt gaccagcgca
     2581 accgtgccgg gcacgaacag cgtcgaggcc acgttgcgac gtgtcccggc ctgggcg
//
LOCUS       DAORNF010000481         2629 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_29473_length_2629_cov_1.607615, whole genome shotgun sequence.
ACCESSION   DAORNF010000481 DAORNF010000000
VERSION     DAORNF010000481.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29473_length_2629_cov_1.607615"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..958)
                     /locus_tag="PLV68_16385"
     CDS             complement(<1..958)
                     /locus_tag="PLV68_16385"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HRE02879.1"
                     /translation="MVGPSRLKRALSLRTRFTLYLALTALATSTLLTAFAFTRARTYL
                     LDQRGAVTRQQAYANANSLLFELRNGDPSTIGTFFLGLRTDESGFAYLYRDGQQRAFL
                     RPGRPESLLPEGFAAKIRMGQSGQQRFSVDGATYVAVGVNINSAGAQYIEVFPLANTE
                     SVIRQIGTALLIGAIVITVLAGGLGWWISRRLLKPVSRVAAAAGGIASGALDTRLQPE
                     SDPELNRLAQSFNDMADTVQTRIEREAQFASDVSHELRSPITALSAAVEVLDARRDDL
                     PERTRQALTVVVDQVRRFDQMVLDLLELSRLDAGVTDLNLEEL"
     gene            complement(1108..1464)
                     /locus_tag="PLV68_16390"
     CDS             complement(1108..1464)
                     /locus_tag="PLV68_16390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02880.1"
                     /translation="MNTPVYCTRVDPATATVVLTVSGPLTSCEHVDLLCEAVNEVPSG
                     RNLIVELDAMTTLSASSLTCLRDLAVKATEKGLRLMLVSESIDVRANLVLADLDSLAP
                     VLHSLAQANQIVAAAA"
     assembly_gap    1747..1841
                     /estimated_length=95
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1842..>2049)
                     /locus_tag="PLV68_16395"
     CDS             complement(<1842..>2049)
                     /locus_tag="PLV68_16395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HRE02881.1"
                     /translation="EVRTEQGVVLKGGQELSLTKTEFRLMCEFAEHAGAVLSRDQLLE
                     RVWGYEYLGDSRLVDAHVRRLRLKV"
     assembly_gap    2050..2241
                     /estimated_length=192
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2242..2449)
                     /locus_tag="PLV68_16400"
     CDS             complement(<2242..2449)
                     /locus_tag="PLV68_16400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRE02882.1"
                     /translation="MQSLLFVEDDDSIRLALRLALEDEGYEVREAHDGRSGLDAFTER
                     EPDLVLVDLRLPDMSGFEVCRSIRA"
BASE COUNT          386 a          815 c          761 g          380 t
ORIGIN      
        1 gcaactcctc caggttcaag tcggtcaccc cggcgtccag gcgggacagt tccagcaggt
       61 cgagcaccat ctggtcgaac cggcgcacct ggtcgaccac caccgtcaat gcttggcgtg
      121 tgcgctcggg cagatcgtca cggcgcgcgt cgagcacctc gaccgccgcg ctcaatgccg
      181 tgatcggcga gcgcaactcg tggctgacgt cggacgcgaa ctgagcttcc cgttcgatgc
      241 gggtctgcac cgtgtcggcc atgtcgttga acgactgggc gaggcggttc aactcggggt
      301 cgctctcggg ttgcaggcgg gtgtcgagcg cgccggaggc gatcccgccc gcagcggccg
      361 cgacgcggct gaccggcttc agcagccggc ggctgatcca ccaccccaac ccaccggcca
      421 gcacggtgat cacgatcgct ccgatcagca gcgccgtgcc gatctggcgg atcacgctct
      481 cggtgttggc cagcgggaat acctcgatgt actgcgctcc ggccgagttg atgttgacgc
      541 cgaccgcgac ataggtcgcg ccgtcgacac tgaagcgctg ctggcccgac tgacccatcc
      601 tgatcttggc cgcgaacccc tcgggcagca ggctctccgg gcggcccggg cgcaggaacg
      661 cccgctgctg gccgtcgcga tagaggtagg cgaagccgct ctcgtccgtt cgcagcccga
      721 ggaagaacgt gccgatcgtg ctcgggtcgc cgttgcgtag ttcgaacagc aacgaattgg
      781 cgttggcgta ggcctgctgg cgcgtcactg cgccgcgctg gtcgagcagg taggtgcgcg
      841 cccgcgtgaa cgcgaacgcc gtcagcaggg tggaggtggc cagcgcggtc aacgccagat
      901 agagcgtgaa gcgcgtccgc aacgacaggg cacgcttgag tcgcgacggg ccaaccacgg
      961 cgccagtctt accgacggcg gcggccgatc gggttctccc cgtgacggcc gtcgcggtcc
     1021 gtcgtgccgc ggtctgtctc gccgcagtgc ctgttgtccc cgtccctgtt gtggtcacga
     1081 tcgctgcggc tgcaccgcac ccccgagcta tgcagccgca gcgacgatct ggttggcctg
     1141 agccaggctg tgcaacaccg gcgccagcga atcgagatcg gccaacacca ggttggcgcg
     1201 gacatcgatc gactcgctga ccagcatgag tcgcaatccc ttctcggttg ccttgaccgc
     1261 caggtcacgc aggcacgtca gactcgacgc ggacagcgtg gtcatcgcgt cgagttcgac
     1321 gatcaggttg cgcccggacg gcacctcgtt caccgcctcg cacagcaggt cgacatgttc
     1381 gcaactggtg agcggaccgg atacggtcaa cacgacggtg gcggtcgcag gatcgactcg
     1441 ggtgcagtag acgggggtgt tcatcaggcc tcctgggcgg tcgagcgcat ctcgacaggt
     1501 ggacagcgtg gctggtggcg agcctccgga tatggaaaga ccgggcaacg ctggcctcca
     1561 ctccgttgtg ggctaccaga ttcccgggac ggttcacccg ctgccggtga ggcaccaggg
     1621 ggaggaaggt acctcaccga agcagcgtga acgagcacca tgatgcggtc gctgtgtgac
     1681 aacccaacga ccgctctgtg cgatttctgt aacgttcttg acgctcggac tggtcactgc
     1741 gccgcgnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngaccttcag ccgcaaccgg
     1861 cgcacatggg catcgaccag gcggctgtca ccgaggtact cgtagcccca cacccgttcc
     1921 agcagctggt cgcgcgacag caccgccccc gcgtgttcgg cgaactcaca catcagccgg
     1981 aactcggtct tggtcaggct cagctcttga ccacccttga gcacgacacc ctgttccgta
     2041 cgcacttcgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn ngcgcccgga tcgagcggca gacctcgaac ccgctcatgt
     2281 cgggcaaccg caggtcgacc agcaccaggt cgggctcgcg ctcggtgaag gcgtcgagcc
     2341 cgctgcgccc atcgtgtgcc tcgcgcactt cgtagccctc gtcctccaac gccaaccgca
     2401 gcgccaagcg gatcgagtca tcatcctcca cgaacaacag cgactgcacg cccccagtct
     2461 gcccgacccg ccacgagtcc ccaagagtgt tcacatcacc cctcgggcgg gggtggatcg
     2521 gacacagtgg cgcccgccag gtcggacggt gttcacttcg cccccgcagc cggggtggat
     2581 cggacacact ctcgcccgcc gggtcggacg gtgttcactt cgcccccgc
//
LOCUS       DAORNF010000482         2628 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_29488_length_2628_cov_1.792849, whole genome shotgun sequence.
ACCESSION   DAORNF010000482 DAORNF010000000
VERSION     DAORNF010000482.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2628
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29488_length_2628_cov_1.792849"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..480)
                     /locus_tag="PLV68_16405"
     CDS             complement(<1..480)
                     /locus_tag="PLV68_16405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRE02883.1"
                     /translation="MATVHRVLPWRRQQHVTHDELAPVLSAYRRRHPKAPTALISKAF
                     ATANEAHRHQVRSSGESYINHPLAVARIVADLGLDETSLAAALLHDSVEDTEITLAEV
                     QRDFGPDVATIVDGVTKLERLQFDSKEAQQAATMRKMLVAMAKDLRVLIIKLADRLHN
                     "
     gene            complement(485..>682)
                     /locus_tag="PLV68_16410"
     CDS             complement(485..>682)
                     /locus_tag="PLV68_16410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HRE02884.1"
                     /translation="DAIHPGERVLIVDDVLATGGTAAATCRLVEALGGVVAGLGFLIE
                     MADLGGRRALGDHKVESLAVY"
     assembly_gap    683..823
                     /estimated_length=141
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<824..1034)
                     /locus_tag="PLV68_16415"
     CDS             complement(<824..1034)
                     /locus_tag="PLV68_16415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HRE02885.1"
                     /translation="MSVETRIIDSAWIKGLVRDIPDYPSPGVIFRDITPLLGNAEGFQ
                     HAVTDLVHRFDGVAVDRAIGMEARGF"
     gene            complement(1087..2277)
                     /gene="secF"
                     /locus_tag="PLV68_16420"
     CDS             complement(1087..2277)
                     /gene="secF"
                     /locus_tag="PLV68_16420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HRE02886.1"
                     /translation="MSTLETGEDAGADGVEMPRARRNVWGRLYHGETRMDFMGRRWLG
                     AGISLALIVVTVISLFAQGLNLGLDFEGGVQWELPSANLTEDQVLGVLNEHGIGTSDA
                     KIQYVTASGVDRIRIQVGEQEPAVRQAVFEDFGALVGNVDDVSSSSVSASWGEEITNK
                     AVRALVVFVILVSIFIAWRFEWRMAVGAIAAMFHDVLISVGVYSVLQLEVTPSTVIAF
                     LTILGFSLYDTIVVFDKIDENTERYGASKVPYDDVINVSMNQVFMRSLNTNITAVLPV
                     VSMLVIGSGIMGAIALRDFSLALFVGLITGSYSSIFIAAPVLAQLKKGTPKWKAANSI
                     PRATGDELERLVLGGLPASRHEKKAKAGTGAAGAATTVSTAAPVTVEQLLTHAPRPRK
                     KTRH"
     gene            complement(2283..>2628)
                     /locus_tag="PLV68_16425"
     CDS             complement(2283..>2628)
                     /locus_tag="PLV68_16425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HRE02887.1"
                     /translation="TYVVFFERLKDDVRLGRQLRNSARRGFKAAFRTVFVANAVSFLG
                     AIVLFYLSVGAVRGFAFYLALSTVMDVIVAYFFTGPIVQLMARSNFMIGKKVMGLEVK
                     APNVSTVKGALS"
BASE COUNT          456 a          805 c          830 g          396 t
ORIGIN      
        1 gttgtgcagc cggtcggcga gcttgatgat caggacccgc aggtccttgg ccatcgcgac
       61 cagcatcttg cgcatcgtgg cggcctgctg ggcctccttc gagtcgaact gcagccgttc
      121 gagcttggtg acgccgtcga cgatggtggc cacgtcgggg ccgaagtcgc gctgcacctc
      181 ggccaaggtg atctcggtgt cttcgaccga gtcgtgcagc aacgccgccg ccagcgacgt
      241 ctcgtcgagg ccgaggtcgg ccacgatgcg ggccaccgcc agaggatggt tgatgtagct
      301 ctcgccgctg ctgcgcacct ggtggcgatg cgcctcgttg gcggtggcga aggccttgga
      361 gatcagcgcc gtcggagcct tgggatgacg ccggcggtag gccgaaagga cgggcgcgag
      421 ctcgtcgtgg gtcacatgct gctggcggcg ccagggcaga acgcgatgga cggtggccat
      481 tgcgtcagta caccgccagc gactcgacct tgtggtcgcc gagggcgcgg cgcccaccca
      541 gatcggccat ctcgatcagg aacccgagtc cggcgacgac accgccgagc gcttcgacca
      601 gacggcaggt tgccgccgcg gtaccgcccg tggccagcac gtcgtcgacg atcaacaccc
      661 gctcgccggg gtggatcgca tcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntgaaccc gcgtgcctcc
      841 atgccgatgg cgcggtcgac ggcgacacca tcgaagcgat ggaccagatc ggtcaccgcg
      901 tgctgaaaac cctccgcatt gccgagcaac ggtgtgatgt cacggaagat gaccccaggg
      961 ctcgggtagt cggggatgtc gcgcacgagc cccttgatcc acgccgagtc gatgatccgc
     1021 gtctccacac tcatggacca aggctaccca gcgggcgagc caccgaacgg gtgcggcgag
     1081 cgatgttcag tgacgggtct tcttgcgcgg ccggggtgcg tgggtgagca actgttcgac
     1141 ggtgaccggc gcggcggtgg acacggtggt ggccgcgcca gcggcacccg tgccagcctt
     1201 ggccttcttc tcgtgacgcg acgccggcag gccaccgagc accagccgtt cgagctcgtc
     1261 accggtggcc cgcgggatgc tgttggccgc cttccacttc ggcgtgccct tcttcaactg
     1321 ggccagcacg ggggcggcga tgaagatcga cgagtacgaa ccggtgatga ggccgacgaa
     1381 cagcgccagg gagaagtcgc gcagcgcgat cgcgcccatg atcccggaac cgatcaccag
     1441 catcgacacc accggcaaca ccgcggtgat gttggtgttg agcgagcgca tgaacacctg
     1501 gttcatcgag acgttgatga cgtcgtcgta gggcaccttg ctcgcgccat agcgctcggt
     1561 gttctcgtcg atcttgtcga acacgacgat cgtgtcgtac aaggagaagc cgagaatggt
     1621 caagaacgcg atcaccgtcg acggggtgac ttcgagttgc agcaccgagt acacgccgac
     1681 gctgatcaac acgtcgtgga acatggccgc gatggcaccg accgccatgc gccattcgaa
     1741 gcgccacgcg atgaagatgc tgaccaagat gacgaacacg accaaagcgc gcacggcctt
     1801 gttggtgatc tcctcacccc aactggccga caccgacgag ctcgacacgt cgtcgacgtt
     1861 gccgaccaac gcgccgaagt cttcgaacac cgcctgacgc acggcgggct cctgctcgcc
     1921 gacctggatc cggatgcggt cgacaccgct ggcggtgacg tactggatct tggcgtcgga
     1981 ggtgccgatg ccgtgctcgt tcaacacgcc gaggacctga tcttcggtga ggttggccga
     2041 gggcagctcc cactgcacgc caccttcgaa gtcgaggccg aggttgaggc cctgggcgaa
     2101 cagggagatc acggtgacga cgatcagcgc caacgagatg ccggcaccga gccagcggcg
     2161 acccatgaag tccatccggg tctcaccgtg atacaggcgg ccccacacgt tgcgccgtgc
     2221 ccggggcatc tcgacgccat cggcgccagc atcctccccg gtttcgaggg tggacacgtc
     2281 ggtcacgaca acgctccctt caccgtggac acgttgggcg ctttgacttc gaggcccatc
     2341 accttcttgc cgatcatgaa gttgctgcgg gccatgagct gcacgatcgg gccggtgaag
     2401 aagtaggcga cgatgacgtc catcaccgtg gacaaagcga ggtagaaggc gaagccgcgc
     2461 accgcgccga cggacaggta gaacagcacg atcgcgccca ggaacgacac cgcgttggcg
     2521 acgaacaccg tgcggaacgc ggccttgaac ccgcgtctgg ccgagttgcg gagctggcga
     2581 cccagcctga cgtcgtcctt caatcgttcg aagaagacga cgtaggtg
//
LOCUS       DAORNF010000483         2627 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_29503_length_2627_cov_2.003888, whole genome shotgun sequence.
ACCESSION   DAORNF010000483 DAORNF010000000
VERSION     DAORNF010000483.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29503_length_2627_cov_2.003888"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1035
                     /locus_tag="PLV68_16430"
     CDS             <1..1035
                     /locus_tag="PLV68_16430"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HRE02888.1"
                     /translation="EHVRAMHGAVRMLGGGAATRITSAAAPRPTPQARPALVPLGERS
                     RLGHRIAAGEFAVSIELGSPVGTDTTKAFGHVDTARAGGIDIINVADGPRAQSRMSNL
                     AFCALAANHAGVEPILHVCCRDRNYLGQIAHLLGAHALGIRNLVVITGDPPKMGDYSF
                     STPVFDVDSIGLLEIATALNAGVDPSGKPLAGATSFVLATGAEPAAIDRDRELERLAA
                     KKAAGAEVVMTQPVYDPATLESFLDDAAELELPIMVGLLPLASHRNAQFLHHEVPGMQ
                     IPQRYLDRMERAGSGPGAQAEGIAIAREALAAVAHRVAGAYIMPPFDRVDAALAILAD
                     VRGTRWHPAP"
     assembly_gap    1091..1100
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     regulatory      1127..1225
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     regulatory      1226..1311
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            1350..>2627
                     /locus_tag="PLV68_16435"
     CDS             1350..>2627
                     /locus_tag="PLV68_16435"
                     /inference="COORDINATES: protein motif:HMM:NF014408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine dehydrogenase"
                     /protein_id="HRE02889.1"
                     /translation="MPTHAHSPATPPKPPAGRADLASLLERDEFVRRHIGPDTAERER
                     MLAAIGVGSLAELIDATVPRSIRTEEPLGVGPGATVEEALGELRQLADKNALRTSLIG
                     MGYYNPLTPPVVQRNVIENPAWYTAYTPYQPEISQGRLEALLNFQTMVAELLDEATAA
                     AEAMTMIRRLTRHKTARFYVDHDTHPQTLTVLHTRAVPLGIELVVGPPSAYDSTCFGA
                     LVSWPASSGELGDHTALIEQVHADGGLVAAITDLLACVLMTPPGHAGADIAVGVSQRF
                     GVPMGFGGPHAAFIATHEANARSLPGRLVGVSTDTAGRPALRLALQTREQHIRREKAT
                     SNICTAQVLLANIAGLYAAWHGPVGLTRIAERVHRLTSITVAGLRANGLEVLNDTWFD
                     TVLVRVGDAERVHIAADVANLALRHIDRFTVGFS"
BASE COUNT          414 a          957 c          871 g          375 t
ORIGIN      
        1 gagcacgtgc gggcgatgca cggcgccgtg aggatgctcg gcggcggcgc ggcaacgcgg
       61 atcaccagcg ccgctgcccc acgtccgacg ccgcaggctc gcccggcgct ggtgccgctc
      121 ggcgagcgca gccgattggg tcaccgcatc gctgcaggtg agttcgccgt gtcgatcgaa
      181 ctcggctcac cggtcggcac cgacaccacc aaggcgttcg gccacgtcga taccgctcgc
      241 gccggaggca tcgacatcat caacgtagcc gacgggccgc gagcgcagtc gcgcatgtcg
      301 aacctggcgt tctgcgcgct cgcggccaac cacgcaggag tcgagccgat cctgcacgtg
      361 tgctgccgcg accgcaacta cctcggccag atcgctcacc tgctcggtgc ccacgccctc
      421 ggcatccgca acctggtggt catcaccggt gacccaccca agatgggcga ttactccttc
      481 tccacacccg tgttcgacgt cgactcgatc ggcctgctcg agatcgccac cgcgctcaac
      541 gccggggtcg acccgtccgg taagccgctt gccggagcga cgtcgttcgt gctcgccacc
      601 ggtgccgaac cggccgcgat cgatcgcgac cgcgagctcg aacgtttggc ggccaagaag
      661 gcggctggtg ccgaggtggt gatgactcaa ccggtgtacg acccggcgac gctcgagtcg
      721 ttcctcgacg acgccgccga gttggagcta ccgatcatgg tggggttgtt gccgctggcg
      781 tcgcatcgca acgcgcagtt cctgcaccac gaggtgccgg gcatgcagat cccacagcgc
      841 tacctcgatc gcatggagcg ggctgggtcg gggcccggtg cccaggccga aggcatcgcg
      901 atcgcccgcg aagcgctcgc tgcggtggcc caccgggtgg ccggggccta catcatgcct
      961 cccttcgatc gagtcgatgc cgcgctggcg atcctggccg acgtgcgcgg cacccgctgg
     1021 caccccgcgc cctgaacccg ccaggtgggc gggcagccaa gcacgatgct gcggccctca
     1081 gcgggaatgg nnnnnnnnnn agcggtcgct acagtggcat tgccgccacg cccaggcggg
     1141 agagtcccga gcagccccgg ctgcgacggc gccgaaggag caaccacccc ggaacctctc
     1201 aggcacccag gaccgcccgg gtcggcaacg ctggaaagta ggtgctcgcc acctcgccga
     1261 cggggaaagc cggttcgccg gtgaagctct caggcacgtc gacagcgggg gtctcaaccg
     1321 ccgaccccca tcctgcttgg agcctgccga tgccgaccca tgcccactcc cctgccaccc
     1381 cgccgaagcc gcccgctggc cgggcggacc tggcatcgct gctggaacga gacgagttcg
     1441 tgcgtcgcca catcggtccc gacaccgccg agcgggagcg gatgctggcc gcgatcggcg
     1501 tcgggtcgct ggccgagctg atcgacgcca ccgtgccgcg atcgatccgc acggaagaac
     1561 ctctcggtgt cggcccgggg gccacggtcg aagaggcact cggcgaactg cgtcaactgg
     1621 ccgacaagaa cgccctgcgc accagcctga tcgggatggg gtactacaac cccctcaccc
     1681 cgccggtcgt acaacgcaac gtgatcgaga acccggcgtg gtacacggcc tacaccccgt
     1741 accaacccga gatcagccag ggtcggctgg aagcgctgct caacttccag acgatggtcg
     1801 ccgagctgct cgacgaagcg accgctgcgg ccgaggcgat gacgatgatc cgccgactca
     1861 cccgccacaa gacggctcgt ttctacgtcg atcatgacac ccacccgcag accctgaccg
     1921 tgttgcacac ccgcgcggtg ccgttgggca tcgagttggt cgtggggccg ccgagcgcgt
     1981 acgactcgac gtgcttcggc gcgctggtgt cgtggcccgc gagcagcggt gagcttggcg
     2041 accacaccgc gctgatcgaa caggtgcacg ccgacggtgg cctggtcgcg gcgatcaccg
     2101 atctgctcgc ctgtgtactg atgacaccgc ccggccacgc cggcgccgat atcgcggtcg
     2161 gtgtctcgca gcgcttcggc gtgccgatgg gcttcggtgg cccgcacgcg gcgttcatcg
     2221 ccacccacga ggcgaacgcc cgctcgctgc cgggacgcct cgtcggcgtc agcaccgaca
     2281 ccgccgggcg gccagcgctg cgtctggcgc tgcagacccg cgagcagcac attcgccgcg
     2341 agaaggccac cagcaacatc tgcaccgcgc aggtgttgct ggccaatatc gccggtctgt
     2401 acgcggcctg gcacggcccg gtcgggctga cccgcatcgc cgagcgggtc caccgactga
     2461 cgtcgatcac ggtcgcgggg ttgcgcgcca acgggctgga ggtgctcaac gacacctggt
     2521 tcgacacggt actggtgcgc gtcggtgacg ccgagcgggt gcacatcgcc gccgacgtcg
     2581 ccaatctggc gctgcgccac atcgaccgct tcacggtcgg cttcagc
//
LOCUS       DAORNF010000484         2622 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_29570_length_2622_cov_1.735489, whole genome shotgun sequence.
ACCESSION   DAORNF010000484 DAORNF010000000
VERSION     DAORNF010000484.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29570_length_2622_cov_1.735489"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(209..>1932)
                     /locus_tag="PLV68_16440"
     CDS             complement(209..>1932)
                     /locus_tag="PLV68_16440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HRE02890.1"
                     /translation="NERPRGGYEIVVTELPYQTSCSAVAGRIQELVDAGDLDGISDVN
                     DGSSGGKTQLVVQLKRDANPNVVLNNLYKLTQLQTSFGVNMVALVDGVPRTLNLRSAL
                     QGYISHQVEVITRRTKYRLGKALEREHKVEGRIKALDVIDEIIALIRASDDAPSAKEA
                     LMAPPFEFSEIQALDILDMQLRQLTRLSRIDLERELTELRERIIELRRILDDPEVLRT
                     VIIDEMTAIRESFATPRVCAITFDSGEMRVEDLVDDTELVVVMTEAQYVKAVPAVSFK
                     TQGRGGRGVSGGKLKADDIVRQVIFTTAHAFLLFFSNRGKVYRLRALDLPERERTAKG
                     MPIVNLLPLQAGETIEAIIDTRDFASERYLFFATKKGTVKKTAFNEYDSGRRDGLIAI
                     NLRDGDELVRVIETSGSDDIFMVARSGTTTRFNEGEVRPMGRSAGGVRGMKLRGNDEV
                     VSVDVVRDDTALLLITEEGFGKRTSLDEFSTKGRGGMGVIGIKINERKGKVAAAFMVG
                     NDDEIVAVSSGGVTIRMQGRSATGVRVMNLDDGQTVASVAPIFASDEVDDSAGVDDDS
                     TEGSADS"
     assembly_gap    1933..1962
                     /estimated_length=30
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1963..>2248)
                     /locus_tag="PLV68_16445"
     CDS             complement(<1963..>2248)
                     /locus_tag="PLV68_16445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012784100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HRE02891.1"
                     /translation="VPTYDGSRNEPVVLPSRFPNLLVNGSQGIAVGMATNIPPHNLGE
                     VIDATIHLIRHPEATIDDLMQFVKGPDFPTGGSILGRQGIVDAYTTGRGSV"
     assembly_gap    2249..2315
                     /estimated_length=67
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2316..>2622)
                     /locus_tag="PLV68_16450"
     CDS             complement(<2316..>2622)
                     /locus_tag="PLV68_16450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HRE02892.1"
                     /translation="QDEMERSFLDYAMSVIMARALPDVRDGLKPVHRRIIWDMEEQGF
                     RPDRAFVKSARVSGDTMAKYHPHGDGAIYDALVRMAQPFSLRHPLIDFHGNYGSPDFG
                     "
BASE COUNT          387 a          818 c          862 g          458 t
ORIGIN      
        1 ccgccccgag gatgcaggcg cagtgctgcg gcgcgggctg ctcttcgccg ccgcgatcgc
       61 gatgatcgcc aacaccaaca ccaccgcctg gccgtgatcg cggtcgcggt cgcaccgtgc
      121 agagtgattc atgacagggc tctccgttcg ctgaagcaac aggtgaacgg tgggcctctg
      181 acgggcggcc cacgagccga tggtcggatc agctgtcggc cgaaccctcg gtggagtcgt
      241 cgtcaacgcc agcactgtcg tcgacctcgt cgctggcgaa gatcggcgcc accgacgcca
      301 cggtctggcc gtcgtcgagg ttcatcaccc gcacgccggt cgcgctgcgg ccttgcatgc
      361 ggatggtgac gccgccgctg gacacggcga cgatctcgtc atcgttgccg accatgaacg
      421 ccgcggccac cttgcccttg cgctcgttga tcttgatgcc gatcacgccc atgccgccac
      481 gacccttggt ggagaattcg tcgagcgagg tgcgcttgcc gaacccctcc tcggtgatca
      541 gcaacagcgc ggtgtcgtcg cgtaccacgt cgacgctgac gacctcgtcg ttgccgcgca
      601 acttcatgcc gcgcacaccg ccagccgagc gacccatcgg gcggacctcg ccctcgttga
      661 agcgggtcgt ggtgccgctg cgggcgacca tgaagatgtc gtccgacccg ctggtctcga
      721 tgacccgcac caactcgtcg ccgtcgcgca ggttgatggc gatcagcccg tcgcgacggc
      781 cactgtcgta ctcgttgaac gcggtcttct tgaccgtgcc cttcttcgtc gcgaagaaca
      841 ggtagcgctc gctggcgaag tcgcgggtat cgatgatcgc ctcgatcgtc tcgcccgctt
      901 gcaggggcaa caggttgacg atcggcatgc ccttggccgt gcgctcccgc tcgggcaggt
      961 cgagcgcgcg caagcgatac accttgccgc ggttggagaa gaacagcaag aacgcgtgcg
     1021 cggtggtgaa gatcacctgg cgcacgatgt cgtcggcctt gagcttgccg ccgctgacac
     1081 cgcggccacc gcggccctgg gtcttgaagc tgaccgcggg gaccgccttg acgtactggg
     1141 cctcggtcat cacgacgacc agttcggtgt cgtcgaccag gtcttcgacg cgcatctcgc
     1201 cgctgtcgaa ggtgatcgcg cacacccgcg gcgtggcgaa cgactcgcgg atcgccgtca
     1261 tctcgtcgat gatcaccgtg cgcaagacct cgggatcgtc gaggatgcgg cgcagctcga
     1321 tgatccgctc gcgcagctcg gtcagctcgc gctcgaggtc gatgcgactg aggcgggtga
     1381 gctggcgcag ctgcatgtcg aggatgtcga gcgcctggat ctcgctgaac tcgaacggcg
     1441 gcgccatcag cgcttctttg gccgacggcg cgtcgtcgct ggcccggatg agggcgatga
     1501 tctcgtcgat cacgtcgagg gctttgatgc gcccttcgac cttgtgctcg cgctcgagtg
     1561 ccttgccgag gcggtacttg gtacgccggg tgatgacctc gacctggtgg ctgatgtagc
     1621 cctgcagcgc cgatcgcagg ttgagggtgc gcggcacgcc gtcgaccagc gcgaccatgt
     1681 tgacgccgaa gctggtctgc aactgggtga gcttgtacag gttgttgagc accacgttgg
     1741 ggttggcgtc acgcttgagc tgcacgacga gctgggtctt gccacccgac gacccgtcgt
     1801 tgacgtcgct gatgccgtcg agatcgccgg cgtcgaccaa ctcctggatg cgccccgcga
     1861 ccgccgagca actggtctgg tagggcaact cggtgaccac gatctcgtaa ccaccacggg
     1921 gccgctcgtt gannnnnnnn nnnnnnnnnn nnnnnnnnnn nntcacgctg ccgcgtccgg
     1981 tggtgtaggc gtcgacgatg ccctggcgac cgaggatcga cccacccgtc gggaagtcgg
     2041 ggcccttgac gaactgcatc aggtcgtcga tggtggcctc gggatgacgg atgagatgga
     2101 tcgtcgcgtc gatgacctcg cccaggttgt ggggcgggat gttggtggcc atgccgacgg
     2161 cgatgccctg actcccgttg accagcaggt tggggaagcg cgacggcaac acgaccggct
     2221 cgttgcggct gccgtcgtag gtgggcacnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngccga agtcgggtga gccgtagttg
     2341 ccgtggaaat cgatcagcgg atggcgcaac gagaacggct gggccatgcg cacgagggcg
     2401 tcgtagatcg ccccgtcgcc gtgggggtgg tacttggcca tggtgtcgcc gctgacgcgc
     2461 gccgacttga cgaaggctcg atcagggcgg aagccctgtt cttccatgtc ccagatgatg
     2521 cggcggtgca ccggcttgag cccgtcgcgc acgtcgggca gggcgcgggc catgatgacc
     2581 gacatcgcgt agtcgaggaa gctgcgctcc atctcgtctt gc
//
LOCUS       DAORNF010000485         2615 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_29675_length_2615_cov_1.592578, whole genome shotgun sequence.
ACCESSION   DAORNF010000485 DAORNF010000000
VERSION     DAORNF010000485.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2615
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29675_length_2615_cov_1.592578"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    211..225
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            293..781
                     /locus_tag="PLV68_16455"
     CDS             293..781
                     /locus_tag="PLV68_16455"
                     /inference="COORDINATES: protein motif:HMM:NF016840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HRE02893.1"
                     /translation="MGQRHTWVDEQHQRMDDLIDEYRVALHDALNGLTEEEARRRLVP
                     SKTTLLGLLKHVSFVEGVWFGQAITGRSLADTGIASTVDGSFTLRPTDTIASVQADYR
                     ERWATSRKNLDGRPLDEVVDGRGARPVWALKLQVLRELAQHAGHVDILREQVVAERRP
                     TL"
     gene            1330..1716
                     /locus_tag="PLV68_16460"
     CDS             1330..1716
                     /locus_tag="PLV68_16460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HRE02894.1"
                     /translation="MLITRGGDVWGRMGRVLLGSLFVYGGYHAAKNPGTRPVALEKVG
                     LPGGENLVRVNGAAQALGGAALAVGIVPGAAAAGLIASLVPTTIVGHPFWRETEPVPR
                     QAQTVQFLKNAAMLGGLLLVVSARRL"
     gene            1981..2598
                     /locus_tag="PLV68_16465"
     CDS             1981..2598
                     /locus_tag="PLV68_16465"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HRE02895.1"
                     /translation="MDYQDAQRAFQDRFDTRRLADRLASVAGDDVTPFRDFIEARDMF
                     FIATADASGRPECSYKGGDPGFVRVIDGSTIAFPLYDGNGMFLTAGNLVENPSVGLLF
                     IDFADGTRLRLCGEASIDSEDPLAEAFPGALLVVRVRATAVFPNCRRYVHTHGATDRS
                     VFVPRGDGPPPVPDWKRDQWFAGTLPADDPAHDDSIPSAPSIPHY"
BASE COUNT          369 a          803 c          949 g          479 t
ORIGIN      
        1 ctgatcgctc tggtgggcac cgtggtgttg tcccttctgg tgttgttctt cccagtgcgt
       61 cgagccgtgc gcttccgtcc tggcgaagcc ctccgctacg cctaacaacc aatcgccgcc
      121 gggtcgacgt cacgacgagc gtcaccggca tcgacgggcg cgttgccagc accgttgtca
      181 ccatcggagc ctgacgcgga acgaagcacg nnnnnnnnnn nnnnngtcgc tgatccggcg
      241 gtaggggacg cgcgggtacg ggcgctccca gttggattcg tatggtggtg ctgtgggaca
      301 gcgccatacc tgggtcgacg aacagcatca gcggatggac gacctcatcg acgagtaccg
      361 agtggcgctg cacgacgcac tcaacggatt gaccgaggaa gaggcgcgtc ggcggctcgt
      421 tccgtcgaag accacgctgc tcggcctcct caagcacgtg agcttcgtcg aaggtgtgtg
      481 gttcggccag gcgatcactg gtcgctcgct tgcggacact gggatcgcct ccactgtgga
      541 cggctcgttc accctccggc cgaccgacac gatcgcatcg gtccaggccg attaccgcga
      601 gcgctgggca acgtcgagga agaacctcga tgggcgacct ctcgacgaag tggtcgatgg
      661 gcggggcgcc cgaccggtgt gggcgctgaa gctccaagtc ctgcgcgagc tcgcacaaca
      721 cgccggccat gtcgacatcc ttcgcgagca agtcgtcgcc gaacggaggc cgacactctg
      781 aaccccggca cttcgccggc accgttccgg cgtgtaggag cgtccggtgg tcgctgcgtc
      841 gggccaaagc ggaagccgtg atccggtcgt gggcgctgac gtggagggcg tagcgggaat
      901 ggtcgtcaag cccggggcgt gtcggtccgc cgattggtcg gctggttggc tggtagtcgc
      961 gtgtggggtc gagggtgagg gtgcgtagga cttcgcttgt ggcggcgtgg atgacgccgg
     1021 ggtcgccgac gagcacgagg acgcgggttc gggcgtgggt tcggccgatg ccgaggccgt
     1081 tgtgtcggag ggtgacgcgg ccggccttgt cgatgcggta gtggtcgtcg ctggtgcggt
     1141 cgcggttcgg ggaggctgct ggttgcgtcg tggaggagtt cctcgtggtt cctacacgca
     1201 gctctgggct cgttggcggt gacccaccgg cccgctgggt gtgcgtgggt gggtagggtt
     1261 cgctgagttc atgatggagg ccgttgtggt gagacgttca ttcaggcagg tcgactggtc
     1321 gtggtcttca tgttgatcac tcgcggtggc gacgtgtggg gtcggatggg gcgagtactg
     1381 ctcggatcgc tcttcgtgta cggcggctat cacgcggcca agaatccggg cacgcgaccg
     1441 gtggcgctcg aaaaggtcgg cctcccaggt ggcgagaatc tggtccgcgt caacggggcg
     1501 gctcaagcgc tgggcggggc cgctcttgcc gtcgggattg tccctggagc ggcggccgcc
     1561 ggtttgatcg cgtcgttggt gccgacgacg atcgtcggtc acccgttctg gcgggagacc
     1621 gaaccggtgc cgcgccaggc ccagaccgtg cagttcctga agaacgctgc gatgctcgga
     1681 gggctgctcc tcgtcgtgag tgcccgccgg ctctgaccga cctcggcaag ccgaacggcg
     1741 gagccgagga cagcgcgctc gacgagtcgg ccgatggggt cggttggtgc gtcgaacgtg
     1801 atgcggaagt gagtggcgcg ccaggtgacc gtttcgccga ggctgatttg ccgcggtagc
     1861 tcccgtcgcg ccctgcgctc gctggatgag gagcagagct cctctgttgc gtggcgtgcg
     1921 ggtgatctaa cgtgcggtcg cagcgaccgc gcgaacgaga cgcgagatcg gaggggactc
     1981 atggactacc aggacgcgca acgcgccttc caggatcggt tcgacacgcg tcggctcgcc
     2041 gatcgccttg cctcggtcgc cggtgacgat gtcacaccgt ttcgcgactt catcgaggcc
     2101 cgcgacatgt tcttcatcgc caccgccgac gcgtctggcc gacccgagtg ctcgtacaag
     2161 ggtggcgatc ccggcttcgt ccgtgtcatc gacggctcga cgatcgcgtt cccgctgtac
     2221 gacggcaacg ggatgttcct gaccgcagga aacctcgtcg agaacccatc ggtggggctg
     2281 ctgttcatcg acttcgcgga cgggactcga ctgcggctgt gcggtgaggc gtcgatcgac
     2341 tccgaggacc cgctcgctga ggcgttcccc ggcgcgttgc tggtggtgcg cgtgcgagcc
     2401 accgcggtgt ttccgaactg tcgtcgttac gtccacactc acggcgccac cgatcgctcg
     2461 gtcttcgtgc cgcggggcga tggcccacca ccggtgcccg actggaagcg cgaccagtgg
     2521 ttcgccggga ccttgccggc cgacgacccg gcgcacgacg actcgatccc cagcgccccg
     2581 tcgatcccgc actactgacg gccgtcctgg gagcg
//
LOCUS       DAORNF010000486         2612 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_29731_length_2612_cov_1.505671, whole genome shotgun sequence.
ACCESSION   DAORNF010000486 DAORNF010000000
VERSION     DAORNF010000486.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29731_length_2612_cov_1.505671"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="PLV68_16470"
     CDS             complement(<1..262)
                     /locus_tag="PLV68_16470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HRE02896.1"
                     /translation="MSDDTPDLPDQPDDAADVPAAAGAAGLPDDGDTAVSTQPIPLQD
                     EMERSFLDYAMSVIMSRALPDVRDGLKPVHRRIIWDMDEQGFR"
     gene            complement(281..>706)
                     /locus_tag="PLV68_16475"
     CDS             complement(281..>706)
                     /locus_tag="PLV68_16475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223523.1"
                     /note="negatively supercoils closed circular
                     double-stranded DNA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HRE02897.1"
                     /translation="IILADADVDGSHIRTLLLTFFYRQMKPLVEAGFVYIAQPPLYST
                     EIGKEKIYLKDDAAKARFLEERPNHKKEFQRLKGLGEMDWEELRGTTMAAESRTLLRV
                     ELVDAAVADEIVSVLMGEDVDSRKQFIQTNAKDVRFLDI"
     assembly_gap    707..846
                     /estimated_length=140
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<847..>1082)
                     /locus_tag="PLV68_16480"
     CDS             complement(<847..>1082)
                     /locus_tag="PLV68_16480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439568.1"
                     /note="negatively supercoils closed circular
                     double-stranded DNA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HRE02898.1"
                     /translation="EANKTVKKAIAASQARLAAKNARNAIRRKTALAGAGMPDKLKDC
                     TSSNPDESELFIVEGDSAGGTALDARDPRTQAIL"
     assembly_gap    1083..1240
                     /estimated_length=158
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1241..>1814)
                     /locus_tag="PLV68_16485"
     CDS             complement(<1241..>1814)
                     /locus_tag="PLV68_16485"
                     /inference="COORDINATES: protein motif:HMM:NF012430.2"
                     /note="negatively supercoils closed circular
                     double-stranded DNA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HRE02899.1"
                     /translation="VDRGGVRYQQRYADGGKPQGPMQQIGTTPADGRTSGTSVTFWPD
                     PIIFHAEGTDFVARTVLERLQTTAFLNRGLIINFIDQREGREQTVTYQYKGGVVDFVR
                     HLNASKEALFTKVAHYEDTDDVEGQTLDIALQWNTGYYEGIHGYANGISTTEGGMHVE
                     GFRTALTSVINKYARAKNLLKEKDDNLLGED"
     assembly_gap    1815..1824
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1825..>2206)
                     /locus_tag="PLV68_16490"
     CDS             complement(<1825..>2206)
                     /locus_tag="PLV68_16490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRE02900.1"
                     /translation="KDIQVLEGLDPVRKRPGMYIGSTGLAGLHHLIWEVVDNSVDEAM
                     AGYCTTVTVTLLADGGCRVDDDGRGIPVDPYPSGPHQGKSAAEVVLTVLHAGGKFGGE
                     GYKVSGGLHGVGVSVVNALSTRLEV"
     assembly_gap    2207..2216
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          323 a          710 c          812 g          449 t
ORIGIN      
        1 ggcggaagcc ctgctcgtcc atgtcccaga tgatgcggcg gtgcaccggc ttcaggccgt
       61 cgcgcacgtc gggcagcgcg cgcgacatga tgacggacat cgcgtagtcg aggaagctgc
      121 gctccatctc gtcttgcagc gggatcggct gggtggacac ggcggtgtcg ccgtcgtcgg
      181 gcaggccggc cgcgcccgct gcagcgggca cgtcagcggc gtcgtcgggt tgatcgggga
      241 ggtcaggggt gtcgtcggac atgtgcttct cgcgtctcga tcagatgtcc aggaaccgga
      301 cgtctttggc gttggtctgg atgaactgct tgcggctgtc gacgtcttcg cccatcaaca
      361 cactgacgat ctcgtcggcc accgccgcgt cgaccagttc gacccgcaac aaggtccggc
      421 tctcggccgc catggtggtg ccgcgcagct cttcccagtc catctcgccc aggcccttca
      481 ggcgctggaa ctccttcttg tggttggggc gctcctccag gaagcgggcc ttggccgcgt
      541 cgtctttgag gtagatcttc tccttgccga tctcggtcga atacagcggc ggctgggcga
      601 tgtagacgaa cccggcttcg accagcggct tcatctggcg gtagaagaac gtgagcagca
      661 gcgtgcggat gtggctgccg tcgacgtcgg cgtcggcgag gatgatnnnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      841 nnnnnngcag gatcgcctgc gtgcgcgggt cgcgcgcgtc gagcgcggta ccgccggccg
      901 agtcgccctc gacgatgaac agttcgctct cgtcggggtt cgagctggtg cagtccttca
      961 gcttgtcggg catgcccgcg ccggcgaggg cggtcttgcg gcggatcgcg ttgcgggcgt
     1021 tcttggccgc gaggcgggcc tgtgacgcgg cgatcgcctt cttgacggtc ttgttggcct
     1081 cgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gtcttcgccg agcaggttgt
     1261 cgtccttctc cttgagcagg ttcttggccc gggcgtactt gttgatcacg ctggtgagcg
     1321 cggtgcggaa gccttcgacg tgcatgccac cttcggtggt ggagatgccg ttggcataac
     1381 cgtggatgcc ctcgtagtag cccgtgttcc attgcagcgc gatgtcgagg gtctggcctt
     1441 cgacgtcgtc ggtgtcttcg tagtgggcga ccttggtgaa cagcgcttcc ttggaggcgt
     1501 tgaggtgacg gacgaagtcg acgacaccgc ccttgtactg ataggtgacc gtctgttcgc
     1561 ggccttcgcg ctggtcgatg aagttgatga tgagaccacg gttcaagaac gcggtggtct
     1621 gcagccgttc gagcacggtg cgagcgacga agtcggtgcc ctcggcatgg aagatgatgg
     1681 gatcgggcca gaaggtgacc gacgtaccgc tggtgcggcc gtcggccggg gtggttccga
     1741 tctgctgcat gggaccctgc ggcttgccgc cgtcggcata gcgctgctgg tagcgcacgc
     1801 cgccacggtc gaccnnnnnn nnnngcacct cgagccgcgt gctgagcgcg ttgaccacgc
     1861 tgacgccgac gccgtgcaga ccgccggaga ccttgtagcc ctcgccgccg aacttgcccc
     1921 cggcgtgcaa cacggtgagc accacctcgg ccgcgctctt gccctggtgc gggccggacg
     1981 ggtacgggtc gaccgggatg ccgcggccgt cgtcgtcgac ccggcagccg ccgtcggcca
     2041 acagggtgac ggtgaccgtg gtgcagtagc cggccatcgc ctcgtcgaca ctgttgtcga
     2101 ccacttccca gatcaagtgg tggagaccgg ccagaccggt ggaaccgatg tacatccccg
     2161 gccgcttgcg caccgggtca agaccttcga ggacctggat gtctttnnnn nnnnnnggcg
     2221 ccgtagtcgc cggtgggctg cacggccgtc gacaccgggt tggatgatgc tgggctcgat
     2281 ggcaccggga ccgcggagtg gtcgtcgggc ggagtgtcag tcaacgacac gcagaacctc
     2341 gtgctttcga tcgttgtgcg gcgattcggg gtcggtcgcg gcatgcgcaa cggccggggc
     2401 cgagtggggt gcggccgacg tggccgaccg ggtgagacca gtctaccaac tgggtgtcac
     2461 acgcgtgtgt tttcgacccc gtcggactcg tggttccgta tcgggttcat ttcgccacgg
     2521 aaacagccga tttttgaccg ctcagcgccg tttgacccgc acgtcgaatc gcgacacctc
     2581 caaaccggct acggaccgca gacggtcggt ga
//
LOCUS       DAORNF010000487         2612 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_29733_length_2612_cov_1.354321, whole genome shotgun sequence.
ACCESSION   DAORNF010000487 DAORNF010000000
VERSION     DAORNF010000487.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29733_length_2612_cov_1.354321"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>357)
                     /locus_tag="PLV68_16495"
     CDS             complement(<1..>357)
                     /locus_tag="PLV68_16495"
                     /inference="COORDINATES: protein motif:HMM:NF021599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2332 family protein"
                     /protein_id="HRE02901.1"
                     /translation="VATVAGHRDEFAIGMRRNVQTNEIGRAAVIASAFSLIAQRHGLA
                     VDQLEIGASGGLMSQWYRYGYETGESFGGAPDSSVQFGPEWWTTPLPSLQPVAVARRR
                     AVDITPIDVTTADGRL"
     assembly_gap    358..372
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<373..736)
                     /locus_tag="PLV68_16500"
     CDS             complement(<373..736)
                     /locus_tag="PLV68_16500"
                     /inference="COORDINATES: protein motif:HMM:NF021599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2332 family protein"
                     /protein_id="HRE02902.1"
                     /translation="MSGPAGSNSHDTSDLARDTGERPSTRTDLASATVADALARQAHS
                     CRLLGSPVAAAVFDAVGADYASGGVTAAVLDGVTSAPIQDAIPLRLLACAHRLALRGD
                     APALARHYESCGWQWDGDA"
     gene            complement(744..>1540)
                     /locus_tag="PLV68_16505"
     CDS             complement(744..>1540)
                     /locus_tag="PLV68_16505"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HRE02903.1"
                     /translation="LLPRRALSIPPFGDLADPRRMATIAAAAEANGWDGIFVWDHLLR
                     PVEQATHIADPWVTMTAMAMVTRTITIGPMVTPIARRRVMKLAREAATLDLASGGRLI
                     LGIGLGVDSGGELIRTAEITDPRERANVLDEALPVLARLLAGEHVVHRGPRVTIDGIT
                     LAPTGLQQPRIPIWLAARGGHLAPVRRAARYEGVCLLDVTPDRYRRITEVIEHERGGT
                     SGYAIAMQSPNPFDPADFPGLAWAIREFPPDVTAAAVEAVVGAPPE"
     assembly_gap    1541..1776
                     /estimated_length=236
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1777..2309)
                     /locus_tag="PLV68_16510"
     CDS             complement(<1777..2309)
                     /locus_tag="PLV68_16510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02904.1"
                     /translation="MSKFYACKGAEASHVGNQISALQMDNPAKAGLGWSNSVFTVTLG
                     GNDLRTKRGDDWPKLLERCILEVNPFGGCHDVTNNQINNWGTVQSSVQATLTQLANAA
                     PNAKIRVMGYPNLMGPTKNWLGIWNCADVTGVTGNEARWIDQQAATLNTVITNAVNAV
                     KAAKPSVNIQFVSVTGQ"
BASE COUNT          348 a          803 c          839 g          371 t
ORIGIN      
        1 ccagtcgtcc gtcggcggtg gtgacgtcga tgggtgtgat gtcgaccgcg cggcggcgag
       61 cgaccgcaac cggctgaagc gatggcaggg gagtggtcca ccactccggg ccgaactgga
      121 ccgagctgtc cggtgcccca ccgaacgact cgccggtctc gtagccgtag cgataccact
      181 gcgacatcag cccgcccgag gcgccgatct ccagttgatc gacggcgagt ccgtggcgct
      241 gagcgatcaa cgagaaagcg ctggcgatca ctgctgcccg cccgatctcg ttggtctgca
      301 cgttgcggcg cataccgatc gcgaactcgt cgcgatgccc ggcgaccgtg gccacgannn
      361 nnnnnnnnnn nncggcgtcg ccatcccact gccagccgca cgactcgtag tgacgggcca
      421 gcgcgggcgc gtcgccgcgc aatgccaacc gatgggcgca ggccaacagc cgcagcggga
      481 tcgcgtcctg aatcggcgcg ctggtgaccc catcgagcac ggccgcggtt acaccgccgc
      541 tggcgtagtc ggcgccgacc gcgtcgaaca ccgcagccgc gaccggcgag ccgagcagac
      601 ggcacgagtg ggcttggcga gcgagcgcgt cggcgacggt cgcactcgcg agatcggtac
      661 gggtcgacgg ccgctcgcct gtgtcgcggg cgagatcgct ggtgtcgtgg gaattgctcc
      721 cggccgggcc gctcacagcc agctcactcg ggtggcgcgc cgaccacggc ttcgacagcg
      781 gcggcggtga cgtcaggcgg gaactcgcgg attgcccacg ccaaacccgg gaagtcggca
      841 ggatcgaacg ggttgggtga ctgcatcgcg atcgcgtagc cactcgtgcc gccccgctcg
      901 tgctcgatca cctcggtgat gcggcggtag cgatcggggg tgacgtccaa caagcagacg
      961 ccttcgtagc gggcggcgcg acgcaccggg gcgaggtggc cgccgcgcgc ggcgagccag
     1021 atcgggatgc gtggctgctg cagaccggtg ggtgccagcg tgatgccgtc gatggtcacc
     1081 cgtgggccac ggtgcacgac atgctcaccg gccaacagcc gggccagcac ggggagcgct
     1141 tcgtcgagca cgttggcccg ttcacgcggg tcggtgatct cggcagtgcg gatcagctcg
     1201 ccgccgctgt cgacgccgag cccgatgccg aggatcagcc gccccccgct ggccagatcg
     1261 agcgtggccg cctcacgggc caacttcatc acccgccgcc gggcgatcgg ggtgaccatc
     1321 gggccgatcg tgatcgtgcg agtcaccatg gccatcgccg tcatcgtcac ccacggatcg
     1381 gcgatgtggg tcgcctgctc gacgggacga aggaggtggt cccagacgaa gatgccgtcc
     1441 cagccgttgg cctccgcggc cgcggcgatc gttgccatcc ggcgcgggtc ggccaagtcc
     1501 ccaaagggtg ggatcgacag cgcccggcgc ggtaagaggc nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnaact gaccggtgac gctgacgaac
     1801 tggatgttga cgctcggctt ggcggccttg accgcgttga ccgcattggt gatgacggtg
     1861 ttcagcgtgg ctgcctgctg gtcgatccag cgggcctcgt tgccggtcac gccggtgacg
     1921 tccgcgcagt tccagatacc gagccaattc ttggtcggcc ccatcaggtt cgggtacccc
     1981 atcacccgga tcttggcgtt gggtgccgcg ttggccaact gggtcagcgt cgcctgcacc
     2041 gacgactgga ccgtgcccca gttgttgatc tggttgttgg tgacgtcatg gcagccgccg
     2101 aacgggttga cctccaagat gcagcgttcg agcagcttgg gccagtcgtc gccacgcttg
     2161 gtgcgcaggt cgttgccgcc gagggtgacg gtgaacaccg agttcgacca gccgagcccg
     2221 gccttggccg gattgtccat ctggagcgcg ctgatctggt tgccgacatg gctcgcctcg
     2281 gcacccttgc aggcgtagaa cttggacacg ccacctcggg ccgcggccaa cttcggcccc
     2341 ggcgtggtct gcagctcgcg ccaacactcc ttgtcgccgc gcgctggcca ccgtactgct
     2401 cgtcgtagct ggagtcctgg gcccagatgc ccgtacccga gctgaacgag tcgccgagcg
     2461 tgaccaccgt gaacgggttc gtgggcggca ccgtgcctgg cggcgccgtg accggggggc
     2521 tggtgatcgg cggcagcttc ggcccgtcga tgatgacccc gccgtcgacc gccgccgtga
     2581 cgcggttgtc cacggcgccc tggtcgacca cg
//
LOCUS       DAORNF010000488         2610 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_29757_length_2610_cov_1.442270, whole genome shotgun sequence.
ACCESSION   DAORNF010000488 DAORNF010000000
VERSION     DAORNF010000488.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2610
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29757_length_2610_cov_1.442270"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="PLV68_16515"
     CDS             complement(<1..202)
                     /locus_tag="PLV68_16515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02905.1"
                     /translation="MVWVPRPRIWTAQPQSPPRLASSGIAQRLIYWTSLHPGARWGIT
                     DLVSRREMGRNAVGGSSMVAGQR"
     assembly_gap    235..252
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<253..666)
                     /locus_tag="PLV68_16520"
     CDS             complement(<253..666)
                     /locus_tag="PLV68_16520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02906.1"
                     /translation="MAAGDPILAVGTTKTLEASGASITNGSVVQADDANYSLSSDAAN
                     WPDAEFVLVCAFATSSSIEGKQVNLYARPLDVQSTNDTEVPEAGRPTHRVGSFTVNGV
                     TSTQYIVLDSLYASDLPRSAAYYLHNETGQTISSGW"
     gene            complement(668..>1113)
                     /locus_tag="PLV68_16525"
     CDS             complement(668..>1113)
                     /locus_tag="PLV68_16525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02907.1"
                     /translation="SLDAAETEQVDAHIADPDDRRDDLIAALLSVGRTEVRATRVSEA
                     GLLERWPAGPLAADAFLAKLEAFASSGHALSGVVRRAMRFLVLPEGLDLGAMATHTMI
                     DALQSGGVITADEAADAKALSAHSAPITVDQVSRALNLAQGRMVI"
     assembly_gap    1114..1142
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1147..1707)
                     /locus_tag="PLV68_16530"
     CDS             complement(1147..1707)
                     /locus_tag="PLV68_16530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02908.1"
                     /translation="MFDIAKLLFATLLAALIGQTAFAQSLKPQQLVTVCTACTAVSAC
                     NTPRLAGDTVALLEWLNGAATPAVLAWRHQVAPVEMDQAANYTAFDSLTAGKRAAWPL
                     MLGYTRDFRVNKTRNAVTDIWGAATAGSVAESILLAGTASATNTQVALGGTAKSTGTV
                     TATDYSYSGPANQLDADWLVQPANCS"
     gene            complement(1719..2120)
                     /locus_tag="PLV68_16535"
     CDS             complement(1719..2120)
                     /locus_tag="PLV68_16535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02909.1"
                     /translation="MAKWANALILDGGSDLLRTRAATTDRIKMHLIKAYTAGDSYSTV
                     VTTNGLGSVSLVAGDIVQSTVSSNRVTTFGAKSITLTANSGASPDLHVAVVDSTTSEV
                     LYVADETTDQVVTSGGTFNVPSFTWTVQQPT"
     gene            complement(2170..>2371)
                     /locus_tag="PLV68_16540"
     CDS             complement(2170..>2371)
                     /locus_tag="PLV68_16540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02910.1"
                     /translation="DTGRIVPPPQPWGWGVEYPTSTGPHRALFLDRARAERAAADLHG
                     RVVVLFEWPLPRDEHVDWSGAA"
     assembly_gap    2372..2381
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2382..>2610)
                     /locus_tag="PLV68_16545"
     CDS             complement(<2382..>2610)
                     /locus_tag="PLV68_16545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02911.1"
                     /translation="EADKAAAQAEIDETLQGRAPESAPESDAAPPDDPAAPAADMQPI
                     IDRLTAIEAALTTATRSAPPTINVSPPAVTVQ"
BASE COUNT          393 a          840 c          868 g          452 t
ORIGIN      
        1 gccgctgccc ggcgaccatc gacgagccgc caacggcatt gcggcccatc tcgcggcgcg
       61 agacaagatc agtgataccc cagcgcgcgc ctgggtgcag gctcgtccaa tagatcagcc
      121 gctgcgcgat gccgcttgat gcgagtctcg gcggcgattg cggctgcgcc gtccagatac
      181 gcggacgcgg aacccagacc atgggcgctg cgcctcagct cgtggccggg atgannnnnn
      241 nnnnnnnnnn nnccacccgc tcgatatcgt ctgcccagtt tcgttgtgca ggtagtacgc
      301 ggccgagcgc ggcaggtcgc tggcgtagag gctgtcgagg acgatgtact gcgtgctcgt
      361 gacgccgttc accgtgaacg agccgacccg gtgtgtcggg cggcctgctt ccggcacctc
      421 agtatcgttc gtgctctgta cgtccagcgg gcgggcgtac aggttcactt gcttgccttc
      481 aatactcgac gaggtcgcaa atgcgcagac cagcacaaat tctgcgtccg gccagttcgc
      541 ggcatcgcta ctcagtgagt agttcgcgtc gtcggcctgc accactgacc cgttcgtgat
      601 ggaagcgccg gacgcctcca gcgtcttggt cgtgcccacg gccagaatgg ggtcgcctgc
      661 ggccatgtca gatcaccatc cttccttgcg cgaggttcag agcgcgcgac acctggtcga
      721 cagtgatggg cgcagagtgc gccgacagcg ctttggcgtc cgccgcctca tcagcggtga
      781 tcacgcctcc ggactgcagc gcgtcgatca tggtatgcgt cgccatcgcg ccaaggtcaa
      841 gcccctcggg caggaccagg aaccgcatgg cgcggcgcac cactccagag agcgcgtgcc
      901 cactcgacgc aaaggcctcg agcttggcca gaaacgcatc ggcagccagt gggcccgcag
      961 gccagcgctc gagcagcccg gcttcagaga cccgcgttgc gcgcacctct gtccggccca
     1021 ccgacagcag cgccgcaatc aagtcgtcgc gccggtcgtc ggggtcggcg atatgggcat
     1081 ctacctgctc agtctcggct gcgtcaagag agcnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nngcggtcat gagcagttcg ccggctggac caaccagtcg gcatccagtt gattcgccgg
     1201 cccagaatag ctgtagtcgg tcgcggtcac ggtgccggtc gacttggccg tcccgccgag
     1261 cgcgacctgc gtattggtcg cactggctgt cccagcgagc agaatcgact cggctacgct
     1321 gcctgccgta gcggcgcccc agatgtcggt caccgcattg cgcgtcttgt tgactctgaa
     1381 gtctcgcgta tagccaagca tcaggggcca cgcagcgcgc ttgccggcag tcagcgaatc
     1441 gaacgcggtg tagttcgcgg cttggtccat ctcgaccggg gcaacttgat ggcgccaagc
     1501 cagcacggcc ggcgttgctg ccccattcag ccactccagc agggccacag tgtcgcccgc
     1561 caagcgtggt gtgttgcacg cgctgactgc cgtgcacgcc gtgcagacag tgaccagctg
     1621 ctgcggcttg agcgactggg cgaaggccgt ctggccgatc aaggcggcta gcagggtggc
     1681 gaacaagagc tttgcgatgt caaacatggt gtcctccttc aagtgggttg ctgcacagtc
     1741 caggtgaaac ttggcacatt gaaggtgccc cctgacgtga ccacctggtc ggtcgtctcg
     1801 tcggcgacgt agagcacctc cgatgtcgtc gagtcgacga cggccacgtg caggtccggg
     1861 ctggcgcccg agttggcggt caacgtgatg gacttggcgc cgaacgtggt gacgcggttg
     1921 ctcgacaccg tgctctgcac gatgtccccg gccacgagac tgaccgagcc caggccgttg
     1981 gtggtgacga cggtcgagta actgtcgccg gccgtgtagg ccttgatcag gtgcatcttg
     2041 atccggtcgg tagtcgccgc gcgggtgcgc agcaggtcac tgccgccgtc gaggatgagt
     2101 gcgtttgccc atttggccat ggtgtggctc cttcagtggt ggttgggtgt gcggtagggg
     2161 ccgggcatgt caggccgccc ccgaccaatc gacgtgttcg tcgcgtggca gcggccactc
     2221 gaacaacact acgacccgcc cgtgcaggtc ggcggcagcc cgctcggccc gcgcgcggtc
     2281 gaggaacagc gcccgatgcg gcccggtcga agttggatac tcgacgcccc agccccacgg
     2341 ctgcggcggc ggaacgatcc gcccggtgtc gnnnnnnnnn nctgcaccgt gacggccggc
     2401 ggcgatacgt tgatcgtcgg cggcgcggag cgggtggccg tcgtcagcgc agcctcgata
     2461 gccgtgagcc tgtcgatgat gggctgcatg tcggctgcgg gcgcagcggg atcgtcgggg
     2521 ggcgcagcgt cgctttccgg cgcgctttcc ggcgcgcgcc cctgcagagt ctcgtcgatc
     2581 tcggcctgtg cggcggcctt gtccgcctcg
//
LOCUS       DAORNF010000489         2603 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_29861_length_2603_cov_1.849686, whole genome shotgun sequence.
ACCESSION   DAORNF010000489 DAORNF010000000
VERSION     DAORNF010000489.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2603
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29861_length_2603_cov_1.849686"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>412)
                     /locus_tag="PLV68_16550"
     CDS             complement(<1..>412)
                     /locus_tag="PLV68_16550"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRE02912.1"
                     /translation="RGEGAILYDAAGERVMVGVHPLEDLAPRDVVAAAISARMASGAA
                     GVDGAAGIDDHVYLDATHLGESFYERFPTITASCLSAGINPARDRIPVAPAAHYVCGG
                     IRATIDGVTELPGLYAVGEVACTGVHGANRLASNS"
     assembly_gap    413..468
                     /estimated_length=56
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<469..>844)
                     /locus_tag="PLV68_16555"
     CDS             complement(<469..>844)
                     /locus_tag="PLV68_16555"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRE02913.1"
                     /translation="HRRIVHAGGDQSGAEVQRTLDQSAIAAGVDVLSHAFALDLVVGT
                     NSVGERQVAGVRIAITRDDDSVESVGVVTARAVVIATGGYGQVFASTSNPQAVTGDGL
                     ALALRAGLPARDIEFVQFHPTVL"
     assembly_gap    845..871
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<872..1252)
                     /locus_tag="PLV68_16560"
     CDS             complement(<872..1252)
                     /locus_tag="PLV68_16560"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRE02914.1"
                     /translation="MTLADEVVGVARVPLQLKAPEVSWQRDVDVVVLGSGAAGLAAAI
                     AARPVRSVLIVTKDTLDAGSTAWAQGGLAGVLDPTDSIDNHVRDTLEAGAGLCDEYAV
                     RELVAEAPKAIRYLIKLGAAFDPGH"
     gene            complement(1256..2323)
                     /gene="nadA"
                     /locus_tag="PLV68_16565"
     CDS             complement(1256..2323)
                     /gene="nadA"
                     /locus_tag="PLV68_16565"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HRE02915.1"
                     /translation="MTTYSDLSGDFDLDTSGACDVDGFCAVPNDSGPFDAAAFAEEVH
                     ELAKARDAVILAHNYQVPEIQEVAHHIGDSLALSRLAATVEQSTIVFCGVHFMAETAK
                     ILAYDKTVLIPDEKAGCSLADIITASQLRAWKAEYPNSVVVSYVNTTAAVKAETDICC
                     TSSNAVEVVSSIPADKTVLFCPDQFLGAHVQRTLQRDNMHIWLGECHVHAGINGADLK
                     QRVMEDPDAELFVHPECGCATSALYLVGAGVVPQERTKILSTSGMVDAARASTAEKIL
                     VATETGMIHQLRKANPLVIFEPVNRAAVCKYMKMITPDKLLASLRNGTDEVTVPRDVA
                     DRARRSVERMIAIGTPSKTGE"
     gene            complement(2333..>2603)
                     /locus_tag="PLV68_16570"
     CDS             complement(2333..>2603)
                     /locus_tag="PLV68_16570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02916.1"
                     /translation="PLRPDGVLAVRASKHPDRRRVRCVAAVADDGIATVLRFEDEPDQ
                     VVAEAGRGIGDVIDALEAMWFGDPTLLTQRFVNMRTTRGGCARRP"
BASE COUNT          399 a          877 c          850 g          394 t
ORIGIN      
        1 gtgagttgct ggccaggcga ttggccccgt gcacccccgt gcaggccacc tcgccaacgg
       61 catacagacc cggtagctcg gtgacgccgt cgatggtggc gcggatgcca ccgcacacgt
      121 aatgggccgc gggtgcaacg ggaatgcggt cgcgagcggg attgatgccc gcgctgagac
      181 acgacgccgt gatggtgggg aaccgctcgt agaacgactc gccgaggtgg gtggcatcga
      241 gatagacgtg atcgtcgatc ccggctgcgc cgtcgacccc ggccgcgccg gacgccatgc
      301 gcgcgctgat cgcggcggcc accacgtcgc gcggtgccag gtcttccaac ggatgcacac
      361 cgaccatgac ccgctccccc gccgcgtcgt acaggatcgc gccctcgccg cgnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnag caccgtcggg
      481 tggaactgca cgaactcgat gtcgcgcgcc ggaagcccag cccgcaatgc caaagccagc
      541 ccgtcgcccg tgacggcctg cgggttggac gtggacgcga acacctgccc gtacccaccg
      601 gtggcgatca cgaccgcccg cgcggtgacc actccgacgc tctccacact gtcgtcgtca
      661 cgggtgatgg cgatgcgtac gccggccacc tggcgctcgc cgacgctgtt ggtgccgacg
      721 accagatcga gggcgaacgc gtggctcagc acgtcgacgc ccgcggcgat ggccgactgg
      781 tcgagcgtgc gctgcacctc ggccccgctc tggtcgccac cggcgtggac gatgcggcgg
      841 tgcgnnnnnn nnnnnnnnnn nnnnnnnnnn ngtgaccggg gtcgaacgcg gccccgagct
      901 tgatgaggta gcggatcgcc ttgggcgctt cggcgaccag ctcgcgcacc gcgtactcgt
      961 cgcacagccc ggcacccgcc tccagcgtgt cgcgcacgtg gttgtcgatc gagtcggtcg
     1021 ggtcgagcac gccggccagg ccaccctgcg cccacgcggt gctgcccgcg tcgagcgtgt
     1081 ccttggtgac gatcaacacc gaccgcaccg gccgagccgc aatcgctgcc gccaagccgg
     1141 cggcacccga acccagcacg accacgtcga cgtcgcgctg ccagctcacc tcgggagcct
     1201 tgagctgcag cggaacgcgg gcgaccccga cgacttcgtc agcgagggtc accggtcact
     1261 cgccggtctt cgacggcgtt ccgatggcga tcatccgctc gaccgaacga cgagcccgat
     1321 cggcgacgtc gcggggcacg gtgacctcgt cggtgccatt gcgcaacgag gccaacagct
     1381 tgtcgggtgt gatcatcttc atgtacttgc acacggcggc ccgattgacc ggttcgaaga
     1441 tgacgagcgg gttggccttg cgcagctggt ggatcatgcc ggtctcggtg gcgaccaaga
     1501 tcttctcggc cgtggacgcc cgggccgcgt cgaccatgcc cgaggtggac aggatcttgg
     1561 tgcgttcctg cggcaccacc ccggcgccga cgaggtacag cgcgctggtg gcgcagccgc
     1621 actcgggatg gacgaacaat tccgcatcgg ggtcttccat cacccgctgc ttgagatcgg
     1681 caccgttgat gcccgcgtgc acgtggcact cgccgagcca gatgtgcatg ttgtcgcgct
     1741 gcaacgtgcg ctgcacgtgc gcgccgagga actggtcggg gcagaacagc acggtcttgt
     1801 cggcagggat actggacacc acctcgaccg cgttcgacga cgtgcagcag atgtcggtct
     1861 cggccttgac cgcggccgtg gtgttgacgt agctgaccac caccgagttg gggtactcgg
     1921 ccttccacgc ccgcaactgc gacgcggtga tgatgtcggc caacgagcag cctgccttct
     1981 cgtcggggat cagcaccgtc ttgtcgtagg cgaggatctt ggcggtctcg gccatgaagt
     2041 gcacgccgca gaacacgatg gtcgactgct cgaccgtggc tgccaaccgg ctgagcgcga
     2101 gcgaatcgcc gatgtgatgc gcgacctctt ggatctcggg cacctggtag ttgtgggcca
     2161 ggatcaccgc gtcgcgcgcc ttggccaact cgtgcacctc ttccgcaaag gctgcggcat
     2221 cgaacgggcc ggaatcattc ggtacggcgc agaacccgtc cacgtcgcaa gcgccggacg
     2281 tgtcgagatc gaagtcaccg gagaggtcgg agtacgtcgt cacgctgcca cgttacggcc
     2341 gtcgcgcgca cccccctcgg gttgttcgca tgttcacaaa gcgctgggtc agcaacgtcg
     2401 ggtcgccgaa ccacatcgcc tccagcgcgt cgatcacgtc accgatgccg cggcccgctt
     2461 cggcaacgac ctgatccggc tcgtcttcga agcgcaacac ggtggcgatg ccatcgtcgg
     2521 ccaccgcagc gacgcagcgc acgcggcggc ggtcgggatg cttgctggcc cgcacggcga
     2581 gcacaccgtc cggtcgcagt ggc
//
LOCUS       DAORNF010000490         2601 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_29899_length_2601_cov_1.863708, whole genome shotgun sequence.
ACCESSION   DAORNF010000490 DAORNF010000000
VERSION     DAORNF010000490.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2601
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29899_length_2601_cov_1.863708"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="PLV68_16575"
     CDS             complement(<1..198)
                     /locus_tag="PLV68_16575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonyl-CoA carboxylase/reductase"
                     /protein_id="HRE02917.1"
                     /translation="MFEGVASADKDPRQSLHIGEVALPEIAPDEAVVAVMASSINFNT
                     VWTSIFEPLPTFGFLDRLGKES"
     assembly_gap    237..251
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            497..1678
                     /locus_tag="PLV68_16580"
     CDS             497..1678
                     /locus_tag="PLV68_16580"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HRE02918.1"
                     /translation="MAGSYIVAGARTPIGKMSGALASFSAADLGGIAIAAALQRAGVA
                     PDEVEHVLMGQVLMAGQGQVPSRQAAVKAGIPMSVPSVNINKVCLSGLNTIYLADQMI
                     QAGEADIVVAGGMESMTNAPYLAPGARGGFRYGNTELADAIIKDGLWCAFDACLMGLG
                     TERYSAGSISREAQDEVAMLSNQRAAAAIATGKLAEEIVEVVIPQRKGDPVVVRDDEG
                     VRASTTMESLAGLKPAFDKEGTITAANASQISDGGSAVVVMSERAVNQRGVTPLGQVV
                     GYGMVAGPDNASLLQQPSKAIFKALSRAGLSVSDVDLFELNEAFAAVGIASMADLGIT
                     AEITNVNGGAIALGHPIGMSGNRLALTVLHELRRRGGGTGAVALCGGGGQGDALLVKT
                     V"
     gene            complement(1929..2534)
                     /gene="recR"
                     /locus_tag="PLV68_16585"
     CDS             complement(1929..2534)
                     /gene="recR"
                     /locus_tag="PLV68_16585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HRE02919.1"
                     /translation="MAAYTQPVQALIDELGRLPGIGPKSAQRIAFHLLKAPGDEVGRL
                     AHAITDAKARVRFCSRCFNFAEGDLCPICSDERRNPAVLCVVEESRDIVALEKTGEFN
                     GRYHVLLGAMNPMEGIGPEQLKMKELFARLMTEPVEEVIICTNPNTEGEVTALYISRH
                     LAQVDIKVTRIASGLPVGGDLEYADELTLGRALTGRRELSA"
BASE COUNT          428 a          833 c          914 g          411 t
ORIGIN      
        1 gctctccttg ccgaggcgat cgaggaagcc gaacgtcggc agcggctcga agatgctggt
       61 ccacaccgtg ttgaagttga tgctgctggc catgaccgcg acgaccgctt cgtcgggcgc
      121 gatctcgggc agcgcgacct cgccgatgtg caggctctgg cgggggtcct tgtcggcgga
      181 ggcgacacct tcgaacatgc ccacgtcctc gcgacggacg aaggcggcac agtaggnnnn
      241 nnnnnnnnnn ngggcagcgg gatggcggcg atgtcgtcac cgctggcccc ggcctcgatc
      301 gcatcgagga tttggcgcat cgggcgatat tacccatggg tagctccgta ttacctatcg
      361 gtagctaacg tctggtcgtc ggtgccgacg ccatgctggc accgattgca gcaggcccag
      421 aagcagggtt tacgctgccc caagcacccg gatcgagcgt cggcgacggc acgatcccca
      481 acgaaggaga gcagcgatgg ctggttcgta catcgtggcc ggagcccgga cacccatcgg
      541 caagatgagc ggcgcgttgg catcgttcag cgccgcagac ctgggcggca tcgcgatcgc
      601 cgccgctctc cagcgcgccg gtgtcgcccc cgacgaagtc gagcacgtgc tgatgggcca
      661 ggtgctgatg gcgggtcagg gccaggtgcc gtcgcgccag gccgcggtca aggcgggcat
      721 tcccatgagc gtgccgtcgg tcaacatcaa caaggtctgc ttgtccgggt tgaacaccat
      781 ctatctggcg gaccagatga tccaggccgg cgaggccgac atcgtggtgg ccggcggcat
      841 ggaaagcatg accaacgcgc cgtacctggc accgggagcg cgcggcgggt tccgctacgg
      901 caacaccgag ctggccgacg cgatcatcaa ggacggcctg tggtgcgcat tcgacgcctg
      961 cctgatggga ctcggcaccg agcgctactc cgcgggcagc atctcgcgcg aggcgcagga
     1021 cgaggtggcg atgctcagca accagcgcgc cgcggccgcg atcgcgacgg gcaagctggc
     1081 cgaggagatc gtcgaagtcg tgatcccaca gcgcaagggt gacccggtcg tggtccgcga
     1141 cgacgagggc gtgcgcgcca gcaccacgat ggaatcgctc gccgggctga agcccgcctt
     1201 cgacaaggag ggcacgatca ccgccgccaa cgcgagccag atcagcgacg gcgggtcggc
     1261 ggtcgtggtg atgagcgaac gggccgtcaa ccagcgcggc gtcacgccgc tgggccaggt
     1321 cgtcggctac ggcatggtgg ccgggccgga caacgcctca ctgctgcagc agccgagcaa
     1381 ggcgatcttc aaggcgctgt cgcgggccgg gttgagcgtg agcgacgtcg acctgttcga
     1441 gctcaacgaa gcctttgccg cggtcggcat cgcgtcgatg gccgacctgg gcatcacggc
     1501 cgagatcacc aacgtcaacg gcggcgcgat cgcgctcggt caccccatcg gcatgagcgg
     1561 caaccgcttg gcgctcacgg tcctgcacga actgcgtcgc cgcggtggcg gcaccggcgc
     1621 ggtcgccctc tgcggcggtg gcggccaagg cgatgccctc ctcgtcaaga cggtgtaagc
     1681 gttcgtgacc ggcgggtttg cgcagcgctg accgactctt ggcacgatct tggggtgacg
     1741 gtcgttgaca tgagaccgca atattcgtaa cgtgactgca acatagtgct ggctcatcga
     1801 ggggctcctc ccccgggtcg gcgcggcaac tgaggcatcg cctcgtgagc tggcccgtcc
     1861 gcccgctacc agctcacggg gtggtgcctc agcaacaatc aaggcgggtt gccccggtcg
     1921 gcgaacggtc aggcggagag ttcgcgcctg ccggtgaggg cgcggccgag ggtgagctcg
     1981 tcggcgtatt ccaagtcgcc gcccacgggg agtccgctgg cgatgcgggt gaccttgatg
     2041 tcgacctggg ccaggtgccg cgagatgtac agcgcggtca cctccccttc ggtgttgggg
     2101 ttggtgcaga tgatgacctc ctcgaccggt tcggtcatga ggcgagcgaa cagctccttc
     2161 atcttcagtt gctcgggacc gatgccctcc atcgggttca tcgctccgag cagcacgtgg
     2221 tagcgcccgt tgaactcgcc ggtcttctcg agggcgacga tgtcgcgcga ctcctccacc
     2281 acgcacaaca cggcggggtt gcgccgctcg tcggagcaga tcgggcacag gtcgccctcg
     2341 gcgaagttga agcaccgcga gcagaaccgg acccgggcct tggcatcggt gatggcgtgg
     2401 gccaatcgac cgacctcgtc acccggcgct ttgagcaggt ggaacgcgat ccgctgggcc
     2461 gacttgggac cgatgccggg caagcgcccg agctcgtcga tgagtgcctg aaccggctgg
     2521 gtgtatgcag ccatggccga tcagtgccgg tcttggacga agtcggaacc ggggaacgcc
     2581 tccatcagct tgtcctccgg g
//
LOCUS       DAORNF010000491         2599 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_29935_length_2599_cov_1.956368, whole genome shotgun sequence.
ACCESSION   DAORNF010000491 DAORNF010000000
VERSION     DAORNF010000491.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2599
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29935_length_2599_cov_1.956368"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="PLV68_16590"
     CDS             complement(<1..417)
                     /locus_tag="PLV68_16590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02920.1"
                     /translation="MLLGVVTIPAIVNVGIGYVTRDRVFDRIEIITYRDYVGVSSALL
                     LFVALVAPDVVCPDRRQHVLPLMFARPLLGRDYVAAKVGAIVTIVFLFSFVPQVVLFV
                     GNMLVSNSAFGYVTDHLDVLWQVPIAVAVLAVQYAVI"
     gene            complement(577..1512)
                     /locus_tag="PLV68_16595"
     CDS             complement(577..1512)
                     /locus_tag="PLV68_16595"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRE02921.1"
                     /translation="MDTRPLLRLRGLTKRYAGVTALDDLSIDVPRGLVGLVGANGAGK
                     TTTFRLLLGLAHPTAGQIEVCGIDVAADPITVRSRLGFMPEHDCLPLDQTAADVVSTF
                     GELSGLPARAARQRASDILDLVGLDESRFRPIGGFSTGMRQRTKLAQALVGDPELVLL
                     DEPTAGLDPLGREEMLTLVSRLGSFGISVMMSTHLLDDVQQVCDHVVMIDGGKLVVSG
                     ATDSLLERTGRVTVDVGAGGAELVEALARLSIAATSAEGLVEVTVDTDADLDRVRDVI
                     DDLRLPLYRLTARLTSLDDVFLHRVGSVPAGGDAG"
     gene            complement(1566..2012)
                     /locus_tag="PLV68_16600"
     CDS             complement(1566..2012)
                     /locus_tag="PLV68_16600"
                     /inference="COORDINATES: protein motif:HMM:NF015862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HRE02922.1"
                     /translation="MSPHLVTKQHITYAAAAAVITAGLAKAAELGTNASIVITDATGE
                     IVAVASTPGANPRGWRGGLGKATAAAGMGISTAAFLEKRLKQDEVLWRALSNNPERMF
                     VPGGIPLKAGGQTVGAVGASGGHYEDDATVAQAVVDAFDALLATDA"
     gene            2243..>2599
                     /locus_tag="PLV68_16605"
     CDS             2243..>2599
                     /locus_tag="PLV68_16605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02923.1"
                     /translation="MTADSNGAATPVSTAALGDGATADRTLRIGYAYPDVAAFAVLND
                     DFSIGDPELQANAVLQAMRRRGELPIGGIDIELVFAKYNILSPSEQLGACTKLAQDDQ
                     VFAVIAGRDFKVGSACL"
BASE COUNT          452 a          841 c          892 g          414 t
ORIGIN      
        1 gatcacggcg tactgcaccg ccaacacagc caccgcgatc ggtacctgcc agagcacatc
       61 gagatggtcg gtgacgtagc cgaacgcgct gttgctgacg agcatgttgc cgacgaacag
      121 caccacctgg ggcacgaacg agaacaggaa cacgatcgtg acgatggccc cgaccttggc
      181 cgcgacgtag tcgcgtccga gcagcggccg ggcgaacatc agtggaagca cgtgctggcg
      241 acgatcgggg cagacgacgt cgggtgccac cagagcgacg aacagcagca atgccgacga
      301 cacacccacg tagtcgcggt aggtgatgat ctcgatccgg tcgaagaccc ggtcgcgggt
      361 cacatagccg atgccgacgt tgacgatcgc gggaatggtc acgacgccga gcagcacgaa
      421 cggggcgatc ttctgccgcc atgctcgccg caacccgagc gcgcgccgga gcgatgcctt
      481 gtagagcgcg aacgtggccg cgctggcctt cgtaggggcg gtacccgcgg tcgtagacag
      541 cgccgcgatc agcgccggaa ccggtcgccg cgaagctcat cctgcatcac cgccggcggg
      601 gacgctgccg acgcgatgca ggaacacgtc gtcgagcgag gtcagccggg ccgtcagccg
      661 gtaaagcggc agacggagat cgtcgatgac gtcacgcacg cggtcgagat cggcatcggt
      721 gtcgacggtg acctcgacca gaccctcggc cgatgttgcc gcgatcgaga ggcgggcgag
      781 tgcctcgacc agttcggccc cgccggcacc gacatcgacg gtgacgcggc cggtgcgttc
      841 gagcagcgag tcggtcgccc ccgacacgac cagcttgccg ccgtcgatca tgaccacgtg
      901 gtcgcacacc tgctgcacgt cgtcgagcag gtgggtcgac atcatcaccg agatgccaaa
      961 gctgccgagg cgggagacga gcgtcagcat ctcctcgcga ccgagcgggt cgagcccggc
     1021 ggtcggctcg tccaacagca ccagttcggg gtcaccgacg agcgcctggg cgagcttggt
     1081 gcgttgacgc atcccggtgg agaacccgcc gatcggacgg aagcgagact cgtccagccc
     1141 gaccagatcg agaatgtcag acgcgcgttg gcgagcagcg cgtgctggca aaccgctcaa
     1201 ctccccgaac gtggacacca cgtcggcagc ggtctggtcg agcggaaggc agtcgtgctc
     1261 gggcatgaac ccgagccggc tgcggacggt gatcgggtcg gcggcgacgt cgatgccgca
     1321 cacttcgatc tgccccgcgg tcggatgcgc cagtccgagc agcaagcgga acgtcgtggt
     1381 cttgccggcc ccgttggcac cgacgaggcc gacgaggcca cgcggcacgt cgatgctgag
     1441 atcgtccagt gcggtcaccc ccgcgtagcg cttggtcagt cccctcagcc ggagcagcgg
     1501 tctcgtgtcc acgacgtgcc cagtctcgca cgcaccgtga ctgacgagcc gtcagtggct
     1561 gcaggtcatg cgtcggtggc cagcagggcg tcgaaggcgt cgacgactgc ttgagccacg
     1621 gtggcatcgt cttcgtagtg gccaccgctg gcaccaacgg cgccgacggt ttgcccgccg
     1681 gccttcagcg ggatgccgcc gggcacgaac atccgctcgg ggttgttcga cagtgctcgc
     1741 cacaagactt cgtcctgctt gagccgcttc tccaggaacg ccgcggtcga gatgcccatc
     1801 ccggccgctg ccgtggcctt gcccaatccg ccacgccatc cgcggggatt ggcccctggg
     1861 gtcgaggcaa ccgccacgat ctctccggtg gcgtcggtga tcacgatcga tgcgttcgtc
     1921 ccaagttcgg ctgccttggc gagcccggcg gtgatcaccg cagccgccgc ggcataggtg
     1981 atgtgctgct tggtgacgag atgtggagac atcggagttc cttgcgtttc agaggtttgg
     2041 gagcagccgt ggtcgtgggg ttttccctga agcgagccga cggacgacga ccggtgggct
     2101 agattcagtc tactgaattc ggcgacgggg ggtttgtcgc cattctcgaa agtgagccgg
     2161 gcgatgcacg cgatgagaca acggagcacg tttctggtcc tgaccatgtt gctcctgtcg
     2221 acgtcggtgg tcgcctgcgc gagtgacggc cgattcgaac ggtgccgcga cgccggtcag
     2281 cactgcagct ctcggtgacg gtgcaacggc cgaccgcacg ctgcggatcg gctatgccta
     2341 cccggacgtc gccgcgtttg cggtgctcaa cgacgacttc tcgatcggtg atccggaact
     2401 gcaggccaat gccgtgttgc aggcgatgcg caggcgcggc gagttgccga tcggggggat
     2461 cgacatcgag ttggtgttcg ccaagtacaa cattctcagc cccagcgaac agctcggtgc
     2521 gtgcaccaag ctggcgcagg acgaccaggt ctttgccgtc atcgccggtc gcgacttcaa
     2581 ggtcggctcg gcgtgtctc
//
LOCUS       DAORNF010000492         2597 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_29977_length_2597_cov_1.463021, whole genome shotgun sequence.
ACCESSION   DAORNF010000492 DAORNF010000000
VERSION     DAORNF010000492.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29977_length_2597_cov_1.463021"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1165
                     /locus_tag="PLV68_16610"
     CDS             <1..1165
                     /locus_tag="PLV68_16610"
                     /inference="COORDINATES: protein motif:HMM:NF013161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRE02924.1"
                     /translation="SIKSFFLRLGNPFTHVAGQHVDVRLTAPDGYTAMRSYSIASSPA
                     SSPILELAIERLHDGEVSPFFHDIAATGDEIELQASVTNATNPELSWEAGQGGWRGDA
                     RLIEAGAILDVPAVWERTHATPSSTSDFPYLITLEATASTGLRADGEPPRRAVVEVRL
                     KELVIEPDPGGVLVNRSIQFVARGADGNVADVTWSATGGTIDANGVYTAGKRPGTYTV
                     TAVSKIDLNIRATATVIVGEADCIAGTWRLRSQDFLDQIGQAVGEPGAYQHLGGEYVV
                     VMSDDGGFRGERRTWQFAVAALGQQLEVTINSVEIGTWSIDAANRTMTIDESSSDATV
                     SMTVNGRELPIGTQAIQSPALSGAATYECDGDVLTTTITDAGTEITATLDRIG"
     assembly_gap    1401..1509
                     /estimated_length=109
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1510..>1760
                     /locus_tag="PLV68_16615"
     CDS             <1510..>1760
                     /locus_tag="PLV68_16615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02925.1"
                     /translation="FPADEYPADVDERETVVQLITVAMPTDELARFLLTELPGAGFTL
                     IDRGHPWLVSEADIQPDVEVWIYVETPDGLPAELHLRPQG"
     assembly_gap    1761..1810
                     /estimated_length=50
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1938..>2150
                     /locus_tag="PLV68_16620"
     CDS             1938..>2150
                     /locus_tag="PLV68_16620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02926.1"
                     /translation="MQTTTSPVGTGAPRGSGIGTTAPCDRVTPAELSAAFDVTFLDGR
                     QIADGQCAFDSEGSPQVLIAVYSSSGG"
     assembly_gap    2151..2363
                     /estimated_length=213
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          383 a          774 c          727 g          341 t
ORIGIN      
        1 gagtatcaag agcttcttcc tgcggttggg gaacccgttc actcatgtcg cgggccagca
       61 tgtcgatgtc cgtctgaccg cgccggacgg ctacaccgcg atgcgaagct attcgatcgc
      121 ctcgtcgccc gcttcatctc cgatcctcga actcgccatc gagcgccttc acgatggcga
      181 ggtctcaccg ttcttccatg atatcgcagc aaccggcgac gagatcgaac tccaggcctc
      241 ggtgacgaac gccaccaacc ccgaactctc gtgggaggcc gggcagggcg gctggcgagg
      301 tgatgcgcgg ctgatcgaag ccggggcgat cctcgacgtg cctgccgtgt gggaacgcac
      361 ccatgccaca cccagctcga cgagcgactt cccctacctg atcacgctgg aagcgaccgc
      421 gtcgaccggg ctgcgggctg atggagaacc tcctcgccgt gccgtggtcg aggtccggct
      481 caaagaactg gtgatcgaac cggatcctgg cggcgtgctc gtcaaccggt cgatccagtt
      541 cgtcgcccgt ggcgccgacg gcaacgtcgc cgacgtgacc tggagcgcga ccggcgggac
      601 catcgacgcc aacggcgtct acaccgccgg gaaacggccg ggcacctaca cggtgactgc
      661 cgtttcgaag atcgacctga acatccgggc gactgcgacc gtgatcgtcg gcgaagccga
      721 ctgcattgca ggcacgtgga ggttgcgcag ccaggacttc ctcgaccaga tcgggcaggc
      781 ggtcggagag cctggggcct accagcacct cggcggcgag tacgtcgtcg tgatgagcga
      841 cgacggcgga ttccgcggcg agcggagaac ctggcagttc gccgtcgcag cgctcggcca
      901 acagctggaa gtgacgatca acagcgtcga aatcggcacc tggtcgatcg acgcggcgaa
      961 ccgcaccatg accatcgacg aatccagctc ggacgcgacc gtctcgatga ccgtgaacgg
     1021 acgagaactc cccatcggca cacaagcgat ccagagccca gcgctcagcg gcgcagccac
     1081 ctacgagtgt gacggtgacg tgctgacgac cacgatcacc gacgccggaa cggaaatcac
     1141 tgcgacactc gaccgcatcg gctgacaccc gtccgcgctt tcaccttcct cacagcgccg
     1201 tcccgtaccg tccgtccccg tgcagacccc atcggacaag catccacggc gcccacccac
     1261 ccacgttcag cgacgcagcc gacgggcgcc cggcgcgacg gttgcggtgc tcctgatcgt
     1321 cctcgtcagc gcgtgcggag gcggtgagga tccgacgacc tccgacagca ggtcgatgga
     1381 tgcgaccgtc gccgaaccga nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnt tcccggctga cgagtacccc gccgacgtcg acgagcgcga aacggtcgtt
     1561 caactcatca cggtggcgat gcccaccgac gaactcgcgc ggttcctgct caccgagctg
     1621 ccaggcgccg ggttcacgct catcgatcgc gggcacccct ggctggtgtc ggaggccgac
     1681 atccaacccg acgtcgaggt gtggatctac gtggaaacac ccgacggact gcctgcggag
     1741 ctccacttgc ggccgcaggg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn ccggtgaccg cgggtgaggc cctccgccgt cgccctgctc gcagcggtcg
     1861 tgttggcgtc gtgcggcgga tccgccgatc ccgcgacagc accgattcag acggctgcat
     1921 cggttccgtc cccgacagtg cagacgacga cgagcccggt cggcaccggg gctcctcgcg
     1981 gctcgggaat cgggacgact gctccatgtg accgtgtcac gccagccgag ctctcggcgg
     2041 cgttcgacgt caccttcctg gacgggcgcc agatcgcgga cggccaatgc gccttcgaca
     2101 gtgaaggttc gccgcaggtg ttgatcgcgg tctactcgtc gagtggcggg nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnccgcagc cttccacgcc catcgagtct gagcactgca
     2401 gtcgcggcgc ggctcgggcc gcgcccgcgg tgggccgtcg aacgtggcga gggctctacg
     2461 gctcggggcg gttcgttgtc tcacgggcag ccgctctcga ccggaaccgg ttggactcgg
     2521 gtggggtcga agaagcgacc ccgcttgccc ttccggatga cgacgtgctc gacgaacagg
     2581 ccgacgaggg cgcgttg
//
LOCUS       DAORNF010000493         2596 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_29995_length_2596_cov_1.508068, whole genome shotgun sequence.
ACCESSION   DAORNF010000493 DAORNF010000000
VERSION     DAORNF010000493.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29995_length_2596_cov_1.508068"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            166..>671
                     /locus_tag="PLV68_16625"
     CDS             166..>671
                     /locus_tag="PLV68_16625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HRE02927.1"
                     /translation="MLDANLTAQLNTYLSNIVHPITLAASLDDSKKSAELRELLDEIA
                     ALSDKITVEQRDDDARRPSFAIERTGTDISVRFAGIPLGHEFTSLVLALLQVGGHPSK
                     VAPELLDQVRDMDVDLTFETYFSLTCQNCPDVVQALNLMSIVNPRIRHIAIDGALFQD
                     EVDARQVMA"
     assembly_gap    672..785
                     /estimated_length=114
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <786..>1275
                     /locus_tag="PLV68_16630"
     CDS             <786..>1275
                     /locus_tag="PLV68_16630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRE02928.1"
                     /translation="KDPFDVLVVGGGPAGAAAAIYAARKGIRTGVVADRFGGQVLDTM
                     AIENFISVSYTEGPKLASALEQHVHEYDVDVMNLQTATSITPASESESGLIEVGLASG
                     ATVQAKTVVLSTGARWRSMNVPGEQEYRNKGVTYCPHCDGPLFKGKRVAVIGGGNSGA
                     EAA"
     assembly_gap    1276..1331
                     /estimated_length=56
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1332..1736
                     /locus_tag="PLV68_16635"
     CDS             <1332..1736
                     /locus_tag="PLV68_16635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRE02929.1"
                     /translation="DDVLQRKMASLPNVDIIVSALTTEARGDGTKLTGLTYTDRTTDQ
                     SYDLDVDGIFVQIGLLPNTDWLRNGPVALSPRGEIEIDQRGQTSVPGVFAAGDCTTVP
                     YKQIVIAMGAGATASLSAFDHLIRTSAPALSA"
     gene            1771..>2596
                     /locus_tag="PLV68_16640"
     CDS             1771..>2596
                     /locus_tag="PLV68_16640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HRE02930.1"
                     /translation="MNLRDLEYLVAVADHRHFRKAAEACFVSQPTLSTQIKKLEHELG
                     VTLLERAPGHVMLTAAGEQVVDRARVMLREAGSILDIARRAGDPESATLRLGLFPTLA
                     PYLLPHVVPALRQAFPKLELLLVEEKTEEVLARLRDGRLDAGILALPVHDDQLHQEYL
                     FDEDFLLALPHGHPLTAAVVDGEVTMDDLHGESLLLLEDGHCLRDQALEVCQLAGAVE
                     RSGFRATSLETLRQMVAAGVGITLLPQLAVRPPVANSPGVTLLRFAEPVPHRSLAMF"
BASE COUNT          397 a          897 c          756 g          376 t
ORIGIN      
        1 ggtcggcaag atctgatcga tctcgcctga tcgagatcac ccagtgagcg ggctggcagc
       61 ccggtaccca catccggact gccagccctt tgctcccacc ctgacatccc cccgtccccg
      121 ttcgctccgc tggcctaccg gccgcgcact ctcgagaagg ccgccatgct cgacgccaac
      181 ctgaccgcgc aactcaacac ctatctcagc aacatcgtcc acccgatcac gctcgctgcg
      241 tcgctcgacg actccaagaa gtcggccgaa ctgcgcgagc tgctcgacga gatcgctgcg
      301 ctgtccgaca agatcaccgt cgaacagcgt gacgacgacg cccgccgccc ttcgttcgcc
      361 atcgagcgca ccggcaccga catcagcgtg cgcttcgccg ggattcctct cggtcacgaa
      421 ttcacgtcgc tcgtgctggc cctgctgcag gtcggcggtc acccgtcgaa ggtcgccccg
      481 gagctgctcg accaggtgcg cgacatggac gtcgacctca ccttcgagac ctacttcagc
      541 ctcacctgcc agaactgccc cgacgtggtg caggcgctga acctgatgag catcgtcaac
      601 ccccgcatcc gccacatcgc gatcgacggc gcgctgttcc aagacgaggt cgacgcccgc
      661 caggtcatgg cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      781 nnnnncaagg accccttcga cgtcctggtc gtcggcggcg gtccggcggg cgcggccgcg
      841 gcgatctacg ccgcccgcaa gggcatccgt accggtgtcg tggccgaccg cttcggtggt
      901 caggtgctcg acacgatggc gatcgagaac ttcatctccg tgtcgtacac cgaaggcccc
      961 aagctggcct cggcgttgga gcagcacgtc cacgagtacg acgtcgacgt catgaacctg
     1021 cagaccgcca cgtcgatcac ccccgccagc gagtctgaga gtgggttgat cgaggtcggc
     1081 ctggccagcg gagccacggt gcaggccaag accgtcgtgc tcagcaccgg cgctcgttgg
     1141 cgctcgatga acgtgcccgg cgagcaggag taccgcaaca agggcgtcac ctactgcccg
     1201 cactgcgacg gcccgctgtt caagggcaag cgggtcgcgg tcatcggcgg cggcaactcc
     1261 ggtgccgaag cagcgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn ngacgacgtg ctccagcgca agatggccag cttgcccaac gtcgacatca
     1381 tcgtgtccgc gctgaccacg gaggcccgcg gcgacggcac caagttgacc gggctcacct
     1441 acaccgaccg caccaccgac cagtcctacg acctcgacgt cgacggcatc ttcgtgcaga
     1501 tcgggctgct gcccaacacc gactggctcc gcaatggccc ggtcgccttg tcgccgcgtg
     1561 gcgagatcga gatcgaccag cgcggtcaga cgtcggtacc cggcgtgttc gcggctggcg
     1621 actgcaccac ggtgccgtac aagcagatcg tgatcgcgat gggcgccggg gcgacggctt
     1681 cgctgagcgc gttcgatcac ctcatccgta cctcggcgcc agcgctgtcc gcttgatccg
     1741 cacccgtacc gagcccgagc cgctgcggcc gtgaacctgc gcgacctcga atacctcgtg
     1801 gcggtcgccg accatcgcca cttccgcaag gcggccgaag catgcttcgt cagccagccc
     1861 acgctgtcga cgcagatcaa gaagctcgag cacgagctgg gcgtgacgct gctcgaacgc
     1921 gctcccggtc acgtgatgct caccgctgcc ggtgaacagg tggtcgaccg ggcgcgcgtg
     1981 atgctgcgcg aagcgggcag catccttgac atcgcccgcc gcgcgggcga ccccgagtcg
     2041 gcgacgctac ggctcggcct gttccccacc ctggcgccgt acctgctgcc gcacgtggtg
     2101 ccggcgctgc gccaggcgtt ccccaagctg gaactgctgc tggtcgaaga gaagaccgag
     2161 gaggtgctgg cccgcctgcg cgacggccgc ctcgacgccg gcatcctggc gctgccggtg
     2221 cacgacgatc aactccacca ggagtacctg ttcgacgagg acttcctcct cgccctcccc
     2281 cacggccatc cgctgaccgc ggcggtggtc gacggcgagg tcacgatgga cgacctgcac
     2341 ggcgagagcc tgttgctgct cgaagacggc cactgcctgc gcgatcaagc actggaggtg
     2401 tgccaactgg ccggtgccgt cgaacggagt ggcttccggg ccaccagctt ggagacactg
     2461 cgccagatgg tggcagccgg cgtcggcatc accttgctgc cccaactcgc ggtgcgcccg
     2521 cctgtggcca actcgcccgg ggtcaccctg ctgcgcttcg ccgagccggt gccccaccgc
     2581 agcctggcca tgttct
//
LOCUS       DAORNF010000494         2595 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_30006_length_2595_cov_1.595276, whole genome shotgun sequence.
ACCESSION   DAORNF010000494 DAORNF010000000
VERSION     DAORNF010000494.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30006_length_2595_cov_1.595276"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..664)
                     /locus_tag="PLV68_16645"
     CDS             complement(<1..664)
                     /locus_tag="PLV68_16645"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HRE02931.1"
                     /translation="MTRAAGLDVGGTKILGVVVDDTGAVIQKRRIATPQGGDAIIGAL
                     ASLARDLGPYDTLGVGIAGLVTRGGVLRASPNLAGSLDVPVGPALVEQLGHPVEVDND
                     ATCAGLAEWRLGAARGHDHVVAVTLGTGVGGGIVLDGRLQRGVNGFAGEFGHMVVEAT
                     GVECVCGQRGCWERYASGSALQRMAGGRRGEDVVAAVRAGDAASAAVIDEFAHWVALG
                     LAN"
     gene            complement(661..1095)
                     /locus_tag="PLV68_16650"
     CDS             complement(661..1095)
                     /locus_tag="PLV68_16650"
                     /inference="COORDINATES: protein motif:HMM:NF015331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HRE02932.1"
                     /translation="MAETASQIISIDAPKDRVYAIAADVERYPEWAKDVKEATVHTRD
                     EQGRPLEVEFRASALGRSTHYTLSYDYSQAPEVIGWSMLRGDIMRSIDGAYTFIDNAD
                     GGVDVRYDLAIELVVPLPGFVRRRAEQRILNTVRELKVRSES"
     gene            complement(1088..>1679)
                     /locus_tag="PLV68_16655"
     CDS             complement(1088..>1679)
                     /locus_tag="PLV68_16655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02933.1"
                     /translation="GQSAPAQPSEQSAAVSPDPPGEPIVRLDLIGTGIFTATAVVAAI
                     VFTPVFRVIGVVVALTLFAIGVVTFLWSYWTAVQRSRTENISVTQLYFLAGGTTPTPV
                     KRTMNLLLAGQCVVGLVTAIARSDTDGRYGSTLAFGILVPVFGLGLNGLWCAQFGNFA
                     PRQPRQATRGDTAAEAGISPAVPHSDDQMGQNADHG"
     assembly_gap    1680..1690
                     /estimated_length=11
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1719..>2595)
                     /locus_tag="PLV68_16660"
     CDS             complement(1719..>2595)
                     /locus_tag="PLV68_16660"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRE02934.1"
                     /translation="LVVLDPALPLGLVGPSLRLPYDVVLHGAEVTVPGRLPASKQTLG
                     YVLRRARHIVAAGGYPAAEARHAAGTELPITVVPPGVDVDRFRPLDAAEHAAARAHFG
                     LAETDEVIVGISRLVPRKGFDTAIRAVAELLPSRPRLKLVIAGAGRDRDRLERLAEEL
                     LAPVRFLGRVSNDDLPRLYGCADLAAMLCRNRWGGLEQEGFGIVFVEAAACGVAQVAG
                     NSGGAAEAVAAGETGLVIDDPRDHHAVARAFAKLLDDDDLRQRMGVASRQRAVEEFSY
                     EVLAERLGKSLGALA"
BASE COUNT          425 a          923 c          885 g          351 t
ORIGIN      
        1 ggttggccag gccgagcgcg acccagtggg cgaactcgtc gatgacggct gcactggcag
       61 catctccggc gcgcaccgcg gcgaccacgt cctcaccacg gcgcccaccg gccatgcgct
      121 gcaatgccga gcccgacgcg tagcgctccc agcagccgcg ctggccgcac acgcactcga
      181 cgccggtcgc ctcgaccacc atgtggccga actcaccggc gaagccgttg acgccacgct
      241 gcaaccgccc gtcgagaacg atccccccgc cgacaccggt gccgagcgtg acggccacca
      301 catggtcgtg accgcgggcc gcacccagac gccactcggc gagcccggcg caggttgcgt
      361 cgttgtcgac ctcgaccggg tggccgagct gttccacgag tgcgggaccg accggcacgt
      421 cgagcgaccc agcgagattg ggtgaggcgc gcagcactcc cccacgggtc accagcccgg
      481 cgatcccgac gcccaacgtg tcgtacgggc cgaggtcgcg ggccagcgat gccagggcgc
      541 cgatgatcgc gtcaccacct tgcggtgttg cgatgcgccg tttctggatc accgcacccg
      601 tgtcgtcgac gacgaccccg aggatcttgg taccgccgac gtcgaggcca gcggcgcggg
      661 tcacgactcg gaacgaacct tcagctcacg cacggtgttg aggatgcgct gctcggctcg
      721 gcgacggacg aagccgggca acggcacgac cagctcgatc gccaggtcgt aacggacatc
      781 gacgccgcca tcggcgttgt cgatgaacgt gtacgcaccg tcgatactgc gcatgatatc
      841 gccgcgcaac atcgaccagc cgatcacctc gggcgcctgg gagtagtcgt acgacagtgt
      901 gtagtgggtg ctgcgcccca gggcagaggc ccggaactcg acctcgagcg ggcggccctg
      961 ctcgtcgcgc gtgtgcaccg ttgcctcttt gacgtccttg gcccactcgg ggtacctctc
     1021 gacatcggcg gcaatggcat agacgcggtc cttcggcgcg tcgatcgaga tgatctggga
     1081 agcggtctca gccatggtct gcattctgcc ccatctggtc gtcggagtgc gggacggctg
     1141 gcgaaatgcc agcctcagcg gccgtgtcgc cgcgcgtggc ctgacgcggt tggcgcggcg
     1201 cgaagttgcc gaactgagcg caccacaagc cgttgagccc caggccgaac accggcacga
     1261 ggatgccgaa ggccagggtc gagccgtagc gcccgtcggt gtcgctgcgg gcgatggcgg
     1321 tcaccaaccc gaccacgcat tggccggcga gcaacaggtt catggtgcgc ttgaccggtg
     1381 tcggtgtcgt gccacccgcc aggaagtaca gctgggtcac cgagatgttc tcggtgcggc
     1441 tgcgctgcac ggcggtccag tagctccaca agaacgtgac cacaccgatc gcgaacagcg
     1501 tcagcgcgac cacgacgccg atcacccgga acaccggggt gaacacgatt gccgcgacca
     1561 ccgcggtcgc ggtgaagatg ccggtcccga tcaggtcgag gcgcacgatc ggctcaccgg
     1621 gcgggtcggg cgacaccgcg gcactctgct cggatggctg ggcgggcgcc gattggcccn
     1681 nnnnnnnnnn atcgttcggc gtcgtcatct gagcgccatc atgccagcgc gccgagcgac
     1741 tttcccagcc gctcggcgag cacctcatag ctgaactcct cgacggcgcg ctgacgtgac
     1801 gcgacgccca tgcgctggcg cagatcgtcg tcgtcgagca acttggcgaa ggcacgggcc
     1861 acggcatggt ggtcgcgcgg gtcgtcgatg accaacccgg tctcaccggc ggcgaccgct
     1921 tcggccgcgc caccggagtt gccggccacc tgggccaccc cgcacgcggc ggcctcgacg
     1981 aacacgatgc cgaacccctc ttgctccagg ccgccccagc ggttgcggca cagcatcgca
     2041 gccaaatcgg cgcacccgta gaggcggggc aggtcgtcgt tgctgacccg gcccaagaag
     2101 cggaccggcg cgagcagttc ctcggcgaga cgctccagac gatcccggtc gcgaccggca
     2161 ccggcgatca ccagcttgag ccgggggcgg gagggcagca gttcggcaac agcgcggatg
     2221 gccgtgtcga accccttgcg cggcaccagg cggctgatgc cgacgatcac ctcgtcggtc
     2281 tcggccaagc cgaagtgagc gcgggccgca gcgtgttcgg cggcatcgag cgggcggaac
     2341 cgatcgacgt cgacccctgg cggcaccacg gtgatcggca gttcggtgcc cgccgcatgg
     2401 cgcgcctcgg ccgccgggta gccgcccgcg gccacgatgt ggcgggcgcg gcgcaacacg
     2461 taccccaacg tctgcttgct ggcgggcaac cggccgggga cggtgacctc ggccccgtgc
     2521 agtaccacgt cgtacggcaa gcgcaacgac gggccgacca agcccaacgg caacgccggg
     2581 tcgagcacca ccagg
//
LOCUS       DAORNF010000495         2593 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_30029_length_2593_cov_2.150118, whole genome shotgun sequence.
ACCESSION   DAORNF010000495 DAORNF010000000
VERSION     DAORNF010000495.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2593
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30029_length_2593_cov_2.150118"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2593
                     /locus_tag="PLV68_16665"
     CDS             <1..>2593
                     /locus_tag="PLV68_16665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02935.1"
                     /translation="ADRVAAELAELGGGHGAYRPLTPARILDTREALGLASSLGPNRS
                     ADLQVNGRGGVPDTGVGAVVLNLTATNPTSSSYVTAWHAGGTRPVASNLNVVAGQTVP
                     NLVIVPVGANGRVSLYNQQGTVDLVADVQGWFPSGSSGYTGAAPFRILDTREGLGVPT
                     ALGQGQSVELQVAGAGAVPATGATAVVLNVTATEGTLPSYITVWPTGATRPVASNLNT
                     VRGGHTVANAVIVPLGTNGKISLFNFAGTTDLIADVQGWFTGDSGLADAESWKIVPAS
                     VLLDANETRQVTLLGVRADGTTFVPHFSEAPTIKTAGSGVSITYVSDGTLRVTAGAAA
                     ATVFAAVQPPGSATSAAMTASIVRLAPGVTVLDDNIVQFPVPYAATGEEQIVALAEDY
                     TTQGIFGFTKAEVGARIHVDTESEPADEFLAPDARFPLIVTGAPPATNSLIITAGGTG
                     VTGRVVEPPGLPTIQRGGYSLVSVEMVNIEMLYDDLEYTITGEQLSDLGFDWYDIEEY
                     VPDDDAASTAEGGAGAEAPTARAESSWSRLADDDTEPPATPEKPKCAFSEGAATVTVD
                     PLVPSITLGADVDFSREREHYKFVPSLTGTIKAGFSVSMQASVGATCEFDLGPHGEIT
                     MRLPGLLAGILNGFAENSVVAKAAISLKFGPTVGFTVRCTSGFTVRAGFEMTRGNVQL
                     RGGFEPIFQCPDTKATFPANSFTDGAFPLSLSISGGKFLRIEVGGRVGGDISRALGYL
                     FSNPELGKAGIVKVDVGPNLTYVQENRPNAMQAKKSSSKLALQLKGTASVTAKGVGWL
                     VKAFGGRAGAVGITLWDQQIDLLTAFAAPAITGMQYQINDEGYKTLAEGETIYAEQGD
                     VVKVRTTL"
BASE COUNT          424 a          899 c          867 g          403 t
ORIGIN      
        1 tgcggaccgc gtcgccgccg aactggccga actgggcggc ggtcacggtg cgtatcgacc
       61 gttgactccg gcgcgcatcc tcgacactcg cgaagcgctc ggtctggcca gctcgctcgg
      121 ccccaaccgg tcggccgacc tccaggtgaa cggccgcggt ggtgtgcccg acaccggtgt
      181 cggtgcggtg gtgttgaacc tgaccgcgac caacccgacg tcgtcgtcgt acgtcaccgc
      241 gtggcacgcg ggcgggacgc ggccggtggc gtcgaacctc aacgtggttg ccggccagac
      301 cgtgcccaac ctggtgatcg tgcccgtcgg cgcgaacggc cgagtgtcgc tgtacaacca
      361 gcagggcacg gtcgatctcg tggccgacgt gcagggctgg ttccccagcg gttcgtccgg
      421 ctacacgggt gcggctccgt tccgcatcct cgacacccgc gagggtctcg gcgtgccgac
      481 ggcactcggc cagggtcaat cggtcgagct gcaggttgcc ggggccggtg cggtgccggc
      541 caccggcgcg accgcggtgg tgctgaacgt gacggctacc gaaggcacac tgccgtcgta
      601 catcacggtc tggccgaccg gcgcgacgcg cccggtggcc tcgaacctca acacggtccg
      661 cggcggtcac accgtggcca atgcggtgat cgttccactc ggaacgaatg gcaagatctc
      721 gctgttcaac ttcgccggca ccaccgatct gatcgccgac gtacaaggtt ggttcaccgg
      781 tgactccggc ctggccgacg ccgagtcgtg gaagatcgtg cccgccagcg tgctgctcga
      841 cgccaacgag acccgccagg tgaccctgct cggcgttcgc gccgacggca ccacgttcgt
      901 accccacttc agcgaagcgc cgacgatcaa gaccgccggg tcgggcgtga gcatcaccta
      961 cgtctccgac ggcaccctgc gcgtcacggc cggtgcagcg gcggccacgg tgttcgccgc
     1021 ggtgcaacca ccggggtcgg cgacgtcggc cgcgatgacc gcctcgatcg tgcgcctcgc
     1081 accaggcgtg acggtgttgg acgacaacat cgtgcagttc cccgtgccgt acgcggcgac
     1141 cggcgaagag cagatcgtcg cgctcgccga ggattacacc acccagggca tcttcggctt
     1201 caccaaggcc gaggtcggcg cccgcatcca tgtcgacacc gagtcagaac cagcggacga
     1261 gttcctcgcg ccggatgccc gcttccctct gatcgtcacc ggcgcaccgc ccgcgaccaa
     1321 ctcgctgatc atcaccgcag gcggcacagg ggtgaccggc cgggtcgtcg agccgcccgg
     1381 cctgcccacc atccagcgcg gcggctacag cctggtgtcg gtcgagatgg tcaacatcga
     1441 gatgctgtac gacgacctgg agtacacgat caccggcgag caactgagcg acctcggctt
     1501 cgactggtac gacatcgagg agtacgtccc cgacgacgac gctgccagta ccgctgaggg
     1561 cggcgcgggt gccgaggcac cgactgcacg agccgagtcg tcgtggtcac gcttggccga
     1621 cgatgacacc gaacccccag ccaccccgga gaagcccaag tgcgcgttca gcgaaggtgc
     1681 ggccacggtg acggtggacc cgttggtgcc cagcatcacc cttggtgctg acgtcgactt
     1741 ctcccgcgag cgtgagcact acaagttcgt gcccagcctg acgggcacca tcaaggctgg
     1801 attctcggtg tcgatgcagg ccagcgtcgg tgccacgtgc gagttcgacc tcggcccgca
     1861 cggcgagatc acgatgcggc tccccggtct gctcgcgggc atcctcaatg ggttcgccga
     1921 gaactcggtg gtggccaagg ccgcgatcag cctcaagttc ggcccgaccg tgggcttcac
     1981 cgtgcgctgc acctctggct tcacggtgcg tgccgggttc gagatgaccc gtggcaacgt
     2041 gcagttgcgc ggcggtttcg agccgatctt ccagtgcccc gacaccaagg cgacattccc
     2101 agccaactcg ttcaccgacg gtgccttccc cttgtcgctc tcgatcagcg gcggcaagtt
     2161 cctgcggatc gaggtgggcg gccgcgtcgg tggcgacatc tcccgcgccc tgggttatct
     2221 gttcagcaat cccgaactgg gtaaggcggg catcgtcaag gtcgacgtcg gtcccaacct
     2281 gacctacgtg caggagaacc ggcccaacgc gatgcaggcc aagaagtcgt ccagcaagtt
     2341 ggcgctgcaa ctcaagggca ccgcgtcggt gacggccaag ggcgtcggct ggctggtcaa
     2401 agcgttcggc gggcgtgccg gggcggtcgg catcacgctg tgggaccagc agatcgacct
     2461 gctcaccgcg ttcgcggccc ccgcgatcac ggggatgcag taccagatca acgacgaggg
     2521 ctacaagacg ctggccgagg gcgagacgat ctacgccgaa cagggcgacg tcgtcaaggt
     2581 gcgcacgacg ctg
//
LOCUS       DAORNF010000496         2582 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_30195_length_2582_cov_1.527899, whole genome shotgun sequence.
ACCESSION   DAORNF010000496 DAORNF010000000
VERSION     DAORNF010000496.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2582
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30195_length_2582_cov_1.527899"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..224)
                     /locus_tag="PLV68_16670"
                     /pseudo
     CDS             complement(1..224)
                     /locus_tag="PLV68_16670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011052967.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     assembly_gap    406..477
                     /estimated_length=72
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <478..>822
                     /locus_tag="PLV68_16675"
     CDS             <478..>822
                     /locus_tag="PLV68_16675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02936.1"
                     /translation="RLEELHAEHGKPVSLLGQSLGGIFARVLARQYPGMVRQVITLGS
                     PYRMMLGDRSSVQRLFEQLSYLHISDLGMTQEAEEHRAPLTMPATSIYSRTDGVAPWQ
                     TCIDKVGPMAENI"
     assembly_gap    823..860
                     /estimated_length=38
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1062..1871)
                     /locus_tag="PLV68_16680"
     CDS             complement(1062..1871)
                     /locus_tag="PLV68_16680"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRE02937.1"
                     /translation="MEASGQYAHGHHPSVLRSHSWRTVANSAAYIAAEFQPGVSVLDV
                     GCGPGTITIDIAQRVAPARVVGIDAAPGVIEVARANAAAAGVTNVSFDVGDCYALDAA
                     DGTFGIVHAHQTLQHLVDPVRALREMRRVAAPGGLIAARDADYGGMLWTPSSGLDEWM
                     RIYQAVHRASAGDPNAGRSMRRWALAAGLDNVATTATVWSFATTDERAWWGGMWAARV
                     TESAFADRAIALGLADREILQRLAQAWHDFVADDTAWFGIPNVEITARVES"
     gene            1995..>2273
                     /locus_tag="PLV68_16685"
     CDS             1995..>2273
                     /locus_tag="PLV68_16685"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:NF014299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HRE02938.1"
                     /translation="MDQSEHGPAARTTARYIALEGAEACGKSTQAARLASVLTAVLTR
                     ETGGTRIGAKIRHILHDNDNTELDDKAEVLLIAADRAQHMAEVVGPALA"
     assembly_gap    2274..2337
                     /estimated_length=64
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2369..>2582
                     /locus_tag="PLV68_16690"
     CDS             2369..>2582
                     /locus_tag="PLV68_16690"
                     /inference="COORDINATES: protein motif:HMM:NF014299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02939.1"
                     /translation="MAIGSRWPDLVILLDVPVGVLAARMAGRDLDRFESAGDAFHHRV
                     RDGFAAMAANDPRHWAVVDGTGSPDEV"
BASE COUNT          382 a          855 c          800 g          371 t
ORIGIN      
        1 catttcacga acccgttgaa ctcctcgctc ttgttggtgg cggcctggat ggtcttgcgg
       61 atttcgatgt cgtcgatgta acgcagcagg aacagggtcc ggaccgcctt gcccagttcg
      121 cggaaggcga agtacagctt gttcttccgg ctgtaggtgc ccaatcgccg caggatggaa
      181 gacgccgtga tcttgcccag ccggatggag accaccaccc gcaggccggt tcgcccgccg
      241 tcacgcacgt tgcaagcgat ggaagcggcc tggctcccgt tcgaggcggc cgggttgtgg
      301 atggccagcc cgttcttgcg cctgatcggc cgcggcgacg accatcccgt gctcgtgctg
      361 cccggcttcg gcgccgacga caactcgttg atcccgctcc gcaggnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncgg
      481 ctggaggagc tgcacgccga gcacggcaag ccggtgagcc tcctcggcca gagcctcggg
      541 ggcatcttcg cccgggtgct cgcccgccag tatcccggaa tggtgcgcca ggtgatcaca
      601 ctgggcagcc cgtaccggat gatgctcggc gaccgcagtt cggtgcagcg cctgttcgaa
      661 caactcagct acctgcacat cagcgacctc ggcatgaccc aggaagccga agagcaccgt
      721 gcgcccctga cgatgccggc cacgtcgatc tattcgcgca ccgacggcgt cgcgccctgg
      781 cagacctgca tcgacaaggt cggcccgatg gccgagaaca tcnnnnnnnn nnnnnnnnnn
      841 nnnnnnnnnn nnnnnnnnnn ccgtcctggt cgccgtgctc gatcgtttgt cgcagccggt
      901 cgacgattgg cgtccgtttg ccccgccgtt cggcctgcgc ggttggtatc cccgaccggc
      961 cgactggacc cccgaacccg tatcgcgccg ccgcgccaca actcgtcgac cgccgacgcg
     1021 ccgctgacct gctcgacgct tgcgaccacc cgaggcgggg gtcaggattc gacgcgggcg
     1081 gtgatctcga cgttggggat gccgaaccag gcggtgtcgt cggcgacgaa gtcgtgccac
     1141 gcctgggcca agcgctgcag gatctcgcga tcggcgagac ccaacgcgat cgcgcgatcg
     1201 gcgaaggccg actcggtcac cctggcggcc cacatgccgc cccaccaggc acgctcatcg
     1261 gtggtggcaa agctccacac cgttgccgtg gtcgccacgt tgtcgagacc tgcagccaac
     1321 gcccagcggc gcatgctgcg ccccgcgttc gggtcccccg cgctggcgcg gtgtaccgcc
     1381 tggtagatcc gcatccattc gtccagcccg gacgacgggg tccacaacat ccctccgtag
     1441 tcggcgtcac gggcggcgat cagaccaccg ggtgctgcga cccggcgcat ctcgcgcaac
     1501 gctcgcaccg ggtcgactag gtgctgcaac gtctggtgag catggacaat gccgaaagtg
     1561 ccatcggccg cgtcgagcgc gtagcagtcg cccacatcga acgacacgtt ggtcacgccc
     1621 gccgccgcgg cgttggcacg agcaacctcg atcacgccgg gcgcggcgtc gataccgacc
     1681 acccgcgccg gagcgacgcg ctgggcgatg tcgatcgtga tcgtgcccgg cccacacccg
     1741 acgtcgagca cgctcacccc tggctggaac tcggcggcga tgtacgcggc cgagttggcg
     1801 acggttcgcc acgagtgcga ccgcaagacc gacgggtgat gtccatgcgc gtattgccct
     1861 gaagcttcca tcctcgttct cccacatgtt gagatctatg tttcacagga tgatacacca
     1921 cgcccggggg ttaacagagg tcgtagccag cccacgggaa gctggacggg cgggcgacgg
     1981 tagggttgcg ccccgtggac cagagcgagc acgggccagc cgcccggacc accgcccgct
     2041 acatcgccct cgaaggtgcc gaggcgtgcg gcaagagcac ccaggcggcg cgactggcga
     2101 gcgtgctgac cgcggtgctc acccgggaga cgggtggcac ccgtatcggc gccaagatcc
     2161 gccacatcct ccacgacaac gacaacaccg agctcgacga caaggccgag gtgctgttga
     2221 tcgccgccga ccgggcccag cacatggccg aggtggtcgg ccccgccctg gccnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngcg
     2341 ccgatgccgg tgacctactt taccgacaat ggcgatcggg tcgcgctggc ccgacctggt
     2401 gatcttgctc gacgtgccgg tcggggtgtt ggccgcgcgc atggccgggc gcgacctcga
     2461 ccggttcgag tcggcaggcg acgccttcca ccaccgcgtg cgcgatggct tcgcggcgat
     2521 ggcggccaac gacccccggc actgggccgt ggtcgacggc accggttcgc ccgacgaggt
     2581 gg
//
LOCUS       DAORNF010000497         2575 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_30292_length_2575_cov_1.612302, whole genome shotgun sequence.
ACCESSION   DAORNF010000497 DAORNF010000000
VERSION     DAORNF010000497.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2575
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30292_length_2575_cov_1.612302"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            55..>550
                     /locus_tag="PLV68_16695"
     CDS             55..>550
                     /locus_tag="PLV68_16695"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRE02940.1"
                     /translation="MEVELLDREAMQAQVNSPTYTGGLWRKDRAALVDPARLVWGLKA
                     AAESLGVTIYEDTKATAIERDGIGVMVHTPLGNVRAGRVALAFKPLLRRIGHYIAPVY
                     DYCMVTEPLNATQLADIGWKQRQGLSDIANEFHYYRLTEDNRILWGGYDAIYFWRGKM
                     NSELE"
     assembly_gap    551..746
                     /estimated_length=196
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <747..962
                     /locus_tag="PLV68_16700"
     CDS             <747..962
                     /locus_tag="PLV68_16700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRE02941.1"
                     /translation="GAEVMLDLLDGKRSKATNTDFVKEKPWPFPPEPFRFAGIQATKW
                     SLQREDRSGKRNLWLRTLDRMGLGFDS"
     assembly_gap    1060..1256
                     /estimated_length=197
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1257..2535)
                     /locus_tag="PLV68_16705"
     CDS             complement(<1257..2535)
                     /locus_tag="PLV68_16705"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HRE02942.1"
                     /translation="MRGADTLGRSPFAVLPHRTPRPPLPRGPRHHPSELVIYETHVRG
                     LTMLAPHVPLAERGTFAGLRHHIEHLVNLGVTAIELLPVHQFDPDEGNYWGYMPLAWS
                     ALHRGYASGDDADDEFAAMVQAMHDAGIEVFLDVVYNHSTEEDDLGPTYHLRGLDDGA
                     YYVLHSDGGYRDDAGCGNVIRAAHPAAQRLILDSLARFADLGVDGFRFDLASLLGRDL
                     GGEPQRDSGLIDAITAFGAARDVRLIAEAWDLSEYQVGDAFPGRSWAQWNGPFRDQAR
                     SFLRAETGGAAGLAQAVAASPQLFGDDWARSVNFITAHDGFTLYDLVSYERKHNLANG
                     QGGLDGTDDNRSWNCGWEGDDVPADLADGVHALRMQQMKNAFVLLLFSAGIPMFVAGD
                     EFAATQGGNNNPYNQDNATTWIDWTRRDRFAELT"
BASE COUNT          357 a          754 c          742 g          329 t
ORIGIN      
        1 gcggacgcct cgtccgccgt gcggagagac taccatcgcc acccggcgct gcgcgtggag
       61 gtcgagctgc tcgaccgcga ggcgatgcag gcgcaggtca actcccccac ctacacgggc
      121 ggcctgtggc gcaaggaccg cgccgcgctg gtcgacccgg cccggctggt gtggggcctc
      181 aaggccgcgg ccgagtcgct cggcgtcacg atctacgaag acaccaaggc caccgcgatc
      241 gaacgggacg gcatcggcgt catggtccac accccgctcg gcaacgtccg cgccggacgc
      301 gtcgcgctgg cgttcaagcc gctgctgcgc cgcatcggcc actacatcgc cccggtgtac
      361 gactactgca tggtgaccga gccgctcaac gccacccagt tggccgacat cggttggaag
      421 cagcgccaag ggttgagcga catcgccaac gagttccact actaccgctt gacggaggac
      481 aaccgcatcc tgtggggtgg ctacgacgcc atctacttct ggcgaggcaa gatgaacagc
      541 gagttggaga nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnnnngggg ccgaggtgat gctcgacctg ctcgacggca
      781 agcgcagcaa ggccaccaac accgacttcg tcaaggagaa gccgtggccg tttccgcccg
      841 aaccgttccg cttcgcaggt atccaggcca ccaagtggtc gctgcaacgc gaagaccgca
      901 gcggtaagcg caacctctgg ctgcgcaccc tcgaccgcat gggcttgggc ttcgacagct
      961 agttcgcccg ctcgagcacc acggtcgagc gggggggcag ggtgatctgg ccgtcgctga
     1021 ccacgagcgt ggcggcaccg gtctgcaacg cgaccgaccn nnnnnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncggt
     1261 caactcggca aagcggtcgc gccgcgtcca gtcgatccac gtggtggcgt tgtcctggtt
     1321 gtaggggttg ttgttgccgc cctgggtggc ggcgaactcg tcaccggcga cgaacatcgg
     1381 gatgcccgcg gagaacagca gcagcacgaa cgcgttcttc atctgctgca tccgcagcgc
     1441 gtgcacaccg tcggccaggt cggcgggcac gtcgtcgcct tcccacccgc agttccacga
     1501 gcggttgtcg tcggtgccgt cgagaccgcc ctgcccgttg gccaggttgt gcttgcgctc
     1561 gtagctgacc aggtcgtaca gcgtgaagcc gtcgtgggcg gtgatgaagt tgacgctgcg
     1621 cgcccagtcg tcgccgaaca actgcgggct tgccgcgacc gcctgcgcca gtccggcggc
     1681 gccaccggtc tcggcgcgca gaaacgaacg ggcctggtcg cggaacggcc cgttccactg
     1741 cgcccacgac cggccgggga acgcgtcgcc gacctggtac tcgctcagat cccatgcttc
     1801 cgcgatcaaa cgcacgtcgc gcgctgcgcc gaacgcggtg atcgcgtcga tcaacccgct
     1861 gtcgcgctgc ggttcgccgc ccagatcgcg gcccagcagc gacgccagat cgaaccggaa
     1921 cccgtcgacg ccgaggtcgg caaagcgcgc cagcgagtcg aggatcaacc gctgcgcggc
     1981 cgggtgcgcg gcgcggatca cgttgccgca accggcatcg tcgcggtacc cgccgtcgga
     2041 gtggagcacg tagtacgcgc cgtcgtccag gccgcgcagg tggtacgtcg ggccgaggtc
     2101 gtcctcttcc gtgctgtggt tgtagacgac gtcgaggaac acctcgatcc cggcatcgtg
     2161 catcgcctgc accatcgccg cgaactcgtc gtcggcatcg tcgcccgacg cgtagccgcg
     2221 gtgcagcgcc gaccaggcca gcggcatgta gccccagtag ttgccctcgt ccgggtcgaa
     2281 ctgatgcacc ggcagcaact cgatcgcggt gacaccgagg ttgaccagat gctcgatgtg
     2341 gtggcgtaac ccggcgaacg ttcctcgctc agcgagcggc acatgcggtg ccagcatcgt
     2401 caggccgcgg acgtgggtct cgtagatcac cagctcgctg gggtgatgtc gcgggccgcg
     2461 gggcagtggt ggacgcggtg tgcgatgcgg caacaccgcg aacggtgagc ggcccagggt
     2521 gtcggctccg cgcacccgcg ccaaggcacg actgtgttct ggcgggaacc acacc
//
LOCUS       DAORNF010000498         2574 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_30308_length_2574_cov_1.936880, whole genome shotgun sequence.
ACCESSION   DAORNF010000498 DAORNF010000000
VERSION     DAORNF010000498.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30308_length_2574_cov_1.936880"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(555..>2574)
                     /locus_tag="PLV68_16710"
     CDS             complement(555..>2574)
                     /locus_tag="PLV68_16710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit R"
                     /protein_id="HRE02943.1"
                     /translation="DGFDLQQETGRAVYADLRIGNIRAAKKDELLELHVPGGQHYLRV
                     NETWGGVEPLAIEREMIRRTIKEHLDKERRFRPMGIKVLSLFFIDAVEKYRIYDAQGN
                     AQQGVYATVFEEEYKRWAGHPDYRDLFGSVDLSAAAADVHNGYFSIDKKKIGGKTIEM
                     VKDTSGSTAADDDTYNLIMRDKEKLLSFDSPLKFIFSHSALKEGWDNPNVFQICNFST
                     RETERWRRQTIGRGLRLCVNQQGHRVRGFEVNTLTVVANESYQQFADSLQKEIEADGY
                     QFGVVPEHLFAGVAVAQDDGTQKPLGFELSKVVAEHLRAAGLVDARGKVKDELRRAIK
                     DGTLSLPPEVEPQRSQILELLRRVSGKIEVKNADERRQVPVRRAVLDSPEFKALWDRI
                     KYKTTYRVQFDNERLIRDCITAVIQAPPISKTRLQWRKAGMEIGEAGVTAVERKGADM
                     VVLNEDDIELPDILTELQNRTQLTRRSIGRILVESHRLNDFKRNPQQFIETVADAINR
                     CKRAVLVDGIRYQRLGDEAYYAQELFETEELTGYLKNMLLDAERSVYQHVIYDSDTER
                     DFADQLSKNESIKVYAKLPGWFKVPTPLGSYNPDWAVLVATPEGDRLYFVVETKSSLF
                     VSDLRNAESAKIECGRAHFKALSVGEKPVIYDKFKDVVGLLERAQSAG"
BASE COUNT          413 a          873 c          768 g          520 t
ORIGIN      
        1 gtctccgcca accccaccgg cccgctgcac gtcggccacg gccgcggcgc ggcgatcggc
       61 gactgcatcg cccgcgtgct cgtcgatgtt ggttagaagt tcatgcagag ccgccgagag
      121 ccggcccgca gcggtcgcct gcacgccaac tgccacgagc tgcgtctgaa acgtcttcgc
      181 gaagtcgttc gacgcggcat ccgccgcggc attgccgccg gcgggtcgat ggatcgctcc
      241 acaggatcct tcgctcacga agagtcggga tgaagccaac agccgacgca gcagtcgccc
      301 gcgtggtgtc gaatcggcaa cagcgatccg tcgatcggcc gtgcggcgca gcatcagcag
      361 ctccaccagt gcacccagac tcgcaccgga ggcatcgacc cgcagggcct cgcgggcacg
      421 ggtcatcatc tgtgcggcgt catcagactg tgcaaatcgc tgctggaatg tcacccgtca
      481 acagcctccg atgccagatc gacacccacg ttgcgaagca ctacagccca ttcaagttcg
      541 ccgggctcaa acagtcatcc cgccgactgg gcccgctcca acagccccac cacgtccttg
      601 aatttgtcgt agatcacggg cttctcgccc actgatagcg ccttgaagtg cgcgcggcca
      661 cattcgatct tggcgctctc ggcgtttcgc agatcgctga cgaacaggct gctcttggtc
      721 tccaccacga agtagagccg atcgccttcc ggcgtcgcca ccagcaccgc ccagtccggg
      781 ttgtagctgc ccaagggtgt gggcaccttg aaccagccag gcagtttggc gtagaccttg
      841 atcgattcgt tcttcgacag ttggtccgcg aagtcgcgct cggtgtcgga gtcgtagatg
      901 acgtgctggt acacggaacg ctcggcatcg agcagcatgt tcttcaggta gcccgtcagc
      961 tcctcggtct cgaacagctc ctgtgcgtag tacgcctcgt cgccaagcct ctggtagcgg
     1021 atgccatcca cgaggacagc gcgcttgcag cggttgatgg cgtcggccac cgtctcgatg
     1081 aactgctgcg ggttgcgctt gaagtcattg agccggtggc tctcaaccag gatgcggccg
     1141 atgctgcgtc tcgtcagctg ggtgcggttc tgcagctcgg tgaggatgtc cggaagttcg
     1201 atgtcgtcct cgttgagcac gaccatgtcg gcgcccttgc gttccaccgc cgtcaccccg
     1261 gcctcaccga tctccatgcc cgccttgcgc cactgcaggc gggttttgct gatgggcggc
     1321 gcctggatca ctgcggtgat gcagtcgcgg atgaggcgtt cgttgtcgaa ctgaactcgg
     1381 tatgtcgtct tgtacttgat gcgatcccag agcgccttga attcggggct gtccagtacg
     1441 gctcggcgca cgggaacctg ccgacgctca tctgcgttct tgacctcgat cttgcccgag
     1501 acccggcgca acagctcgag gatctggctg cgttgcggct ccacctccgg cggcaacgac
     1561 agagtgccgt ccttgatcgc gcggcgcagc tcgtccttga ccttgccgcg cgcgtccacc
     1621 aagcctgcag cacgcagatg ctcggcgacc actttggaga gctcgaagcc caagggcttc
     1681 tgcgtgccat cgtcctgcgc cacggcaacc ccggcgaaca gatgctccgg cacgacgccg
     1741 aactgatacc cgtcggcctc gatttccttc tgcaagctgt cggcaaactg ctggtagctc
     1801 tcgttcgcca ccacggtcag cgtgttcacc tcgaagccgc gcacgcggtg gccctgctgg
     1861 ttgacgcaca gccgcaggcc tcgcccgatg gtctgccgcc gccagcgctc ggtctcgcgc
     1921 gtgctgaagt tgcagatctg aaagacgttc gggttgtccc agccttcctt caacgccgag
     1981 tgactgaaga tgaacttcaa cggcgagtcg aagctcagca gcttctcctt gtcgcgcatg
     2041 atcaggttgt aggtgtcgtc gtcggccgcc gtgcttccgc tggtgtcctt caccatctca
     2101 atggtcttgc cgccgatctt cttcttgtcg atcgagaagt agccgttgtg cacgtcggcc
     2161 gctgcggcac tcaggtccac cgagccgaac aggtcgcggt agtccgggtg tccggcccag
     2221 cgcttgtatt cctcttcgaa gaccgtcgcg tagacgcctt gctgggcgtt gccctgcgca
     2281 tcgtagatgc ggtacttttc caccgcgtcg atgaagaaca ggctgagcac cttgatgccc
     2341 atcggtcgga agcgccgctc cttgtccaga tgctccttga tcgtgcggcg gatcatctcc
     2401 cgctcgatcg ccagcggctc cacgccaccc caggtctcgt tgacgcgcag gtagtgctgc
     2461 ccgccgggca cgtgcagctc cagcagctca tccttcttgg ctgcgcggat gttcccaatc
     2521 cgcagatcag cgtacacggc ccgccccgtc tcttgctgca ggtcgaatcc gtcc
//
LOCUS       DAORNF010000499         2571 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_30352_length_2571_cov_1.661765, whole genome shotgun sequence.
ACCESSION   DAORNF010000499 DAORNF010000000
VERSION     DAORNF010000499.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2571
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30352_length_2571_cov_1.661765"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..526)
                     /locus_tag="PLV68_16715"
     CDS             complement(<1..526)
                     /locus_tag="PLV68_16715"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRE02944.1"
                     /translation="MLQWDHEVDLLVVGSGTGMAAALTGAVKGLTTLLIDKADVYGGT
                     TAISGGGIWVPNNVALARAGVADTPAEAKAYVDAVLALNNDDVSPERRHAYLTRGPEA
                     IEFLEQHASLLTFTWVENYPDYHPELPGGKAEGRQIQTSAIDGRALGDERARMRPPLR
                     LAPQPFGMWIMISEA"
     assembly_gap    623..667
                     /estimated_length=45
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<668..>1007)
                     /locus_tag="PLV68_16720"
     CDS             complement(<668..>1007)
                     /locus_tag="PLV68_16720"
                     /inference="COORDINATES: protein motif:HMM:NF012885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02945.1"
                     /translation="DRVAAVVEGLRGDAQVGFHGHQNLSLGVANTVLAMRAGAVQLDA
                     CTRSFGAGSGNTPTEALAAVLDRLGVRTGIDPLAMFDVAEEIVRPVMDGECVLDRLSL
                     IMGYAGVYSSF"
     assembly_gap    1008..1064
                     /estimated_length=57
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1065..1422)
                     /locus_tag="PLV68_16725"
     CDS             complement(<1065..1422)
                     /locus_tag="PLV68_16725"
                     /inference="COORDINATES: protein motif:HMM:NF012885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02946.1"
                     /translation="MSGLDAAGVPVIEVTHGDGLAGSSFNYGFSLVDEKVLMKAAVDE
                     AKNAKIAALMLPGLGTLDDIRACVDLGVSVVRIATHCTEADISEQHFTIARDLGLETV
                     GFLMMAHKISAEELVPQ"
     gene            complement(1520..>1791)
                     /locus_tag="PLV68_16730"
     CDS             complement(1520..>1791)
                     /locus_tag="PLV68_16730"
                     /inference="COORDINATES: protein motif:HMM:NF006157.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02947.1"
                     /translation="ALQPDADEAVVTASIEEIVAAVQSYVPGYRLRVPPQFDETHGVR
                     RVTIMVEVEGAGDFLPPYAGNLDIMTAAAAKVGDELGRELLKQGR"
     assembly_gap    1792..1837
                     /estimated_length=46
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1838..2413)
                     /locus_tag="PLV68_16735"
     CDS             complement(<1838..2413)
                     /locus_tag="PLV68_16735"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF006157.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase (acetylating)"
                     /protein_id="HRE02948.1"
                     /translation="MTRSKVSAAIVGSGNIGTDLMYKLLRSEVVDLRYMIGVDPSSEG
                     LKRAEAEGLETSAEGVDWLLERPELPDVVFEATSAYVHLGNAPRYRELGIRAVDLTPA
                     AVGPFVIPPVNLTGHLGEMNVNMVTCGGQATIPMVHAVSRVTPVAYAEIIATVSSVSA
                     GPGTRQNIDEFTRTTSNAVVELGGAERGKAII"
BASE COUNT          415 a          794 c          800 g          414 t
ORIGIN      
        1 gagcctcgga gatcatgatc cacatgccga acggttgcgg agcgaggcgc aagggtggcc
       61 gcatccgggc ccgctcgtca cccaacgctc gtccgtcgat cgccgatgtc tggatctggc
      121 gtccctcggc cttgccgccc gggagttcgg ggtgatagtc ggggtagttc tcgacccagg
      181 tgaaggtgag cagtgacgcg tgctgctcga ggaattcgat agcctcgggg ccgcgagtca
      241 ggtaggcgtg gcgacgctcg ggagacacgt cgtcgttgtt gagcgcgagc accgcgtcga
      301 cgtacgcctt ggcttcggcc ggggtgtcgg ccactcccgc gcgggccagc gcaacgttgt
      361 tcggcaccca gattccacca ccggaaatcg cagtggtgcc gccgtagacg tcggccttgt
      421 cgatcagcag tgtcgtcaga cccttcaccg cgccggtgag cgcggcggcc ataccggtac
      481 ccgaaccgac gacgaggagg tcgacttcgt ggtcccactg cagcatgtca gccagctccg
      541 ttctgctcgg cgagttgaac agcgatctcg aggatctggt cttcctggcc tccgacgagg
      601 ttgcgccgac cgcactccat cannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      661 nnnnnnngga agctcgaata gacgcccgcg taacccatga tgaggctcaa ccggtcgagc
      721 acgcactcgc cgtccatcac gggccggacg atctcctccg cgacgtcgaa catcgcaagc
      781 ggatcgatcc cggtgcggac tcccaaccga tccaacaccg cagcgagcgc ttcggtgggc
      841 gtgttgcccg agccggcacc gaatgaccgc gtgcaggcat cgagttgcac cgctccggca
      901 cgcatcgcga gcacggtatt ggcgacgccg agcgacaggt tctgatgtcc gtggaacccg
      961 acttgggcgt caccacggag accttcgacg acggccgcga ccctgtcnnn nnnnnnnnnn
     1021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncctggg gcacgagttc
     1081 ctccgcggag atcttgtgcg ccatcatcaa gaacccgacg gtctcgagtc cgaggtcgcg
     1141 tgcgatggtg aagtgctgct cggagatatc cgcttccgtg cagtgggtgg cgatccgcac
     1201 cacagacacg ccgaggtcga cgcaggcccg gatgtcgtcg agcgtgccca gaccgggcag
     1261 catcaacgcc gcgatcttgg cgttcttggc ctcgtcgacg gcagccttca tgaggacctt
     1321 ctcgtccacc aacgaaaacc cgtagttgaa cgacgatccg gccagaccat cgccatgggt
     1381 gacctcgatc accgggacgc cggcagcgtc gaggccggac accacctggc ggatgtggtc
     1441 ctcggtgaac tggtggggga gccgtcgcgc agggacgaat cggtgatccg aacgtcgaga
     1501 gagtcggaga atggcatggt catcgtcctt gcttgagtag ttcgcggccg agttcgtcgc
     1561 cgactttggc agcggccgca gtcatgatgt cgagattgcc cgcgtacggc gggaggaagt
     1621 cgcccgcccc ctcgacttcg accatgatcg tcacccgccg cactccgtgc gtctcgtcga
     1681 actgtggcgg cacgcgcagg cggtagccgg gaacgtaact ctgcaccgcc gccacgatct
     1741 cctcgatcga ggccgtgacg acggcctcgt cggcgtcggg ttgcagcgcg cnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngat gatcgccttg ccgcgctcgg
     1861 cgccgccgag ttcgaccacg gcgttcgacg tggtgcgagt gaactcgtcg atgttctggc
     1921 gcgtgccggg accggccgac accgacgaga ccgtggcgat gatctccgcg tacgcgaccg
     1981 gcgtcacgcg ggagacggcg tggaccatgg ggatggtcgc ttgcccgccg caggtcacca
     2041 tgttgacgtt catctcgccg agatgaccgg tgagattgac cggtgggatc acgaacgggc
     2101 cgacggccgc aggggtgagg tcgaccgcgc gaatgccgag ctctcgatag cggggcgcgt
     2161 tgcccaggtg cacgtaggcc gacgtggcct cgaacaccac gtcgggaagc tctggacgtt
     2221 cgagcaacca gtcgacacct tcggccgacg tctccaagcc ttcggcttcg gcgcgcttga
     2281 gcccctcgct ggatgggtcg acgccgatca tgtagcgcag atcgacgacc tccgatcgca
     2341 acagcttgta catcaagtcg gttccgatgt tgccggaccc gacgatcgcc gccgagactt
     2401 tcgatctggt cattcaccca tcatccggta cgactcagcg aggcgcatcg acgacgtccc
     2461 agtggacgag acgaagcagc gagtgccgac caacggaacc cggtgcttgc gatcgcacgt
     2521 cgattgggct cgaatcgggt cgtgacgact cccgatctgc tccgccgcgc c
//
LOCUS       DAORNF010000500         2568 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_30404_length_2568_cov_1.693195, whole genome shotgun sequence.
ACCESSION   DAORNF010000500 DAORNF010000000
VERSION     DAORNF010000500.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30404_length_2568_cov_1.693195"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            164..454
                     /locus_tag="PLV68_16740"
     CDS             164..454
                     /locus_tag="PLV68_16740"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HRE02949.1"
                     /translation="MLAIDLGTTGVKVAVVDAEGLALGAAGETLPLLSVADGGVEQDA
                     DGWWQAIGRCARRVLVSVPAARAEVGLIAVTSQYNSTVAVDARGLPVANAVM"
     gene            455..>764
                     /locus_tag="PLV68_16745"
     CDS             455..>764
                     /locus_tag="PLV68_16745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02950.1"
                     /translation="MDGRGRRNHPGVARGADRGDWIERHGMAPNYGDTAGHVATIRAV
                     SPDAYAAAHAFVEPMDAIAARLTGHVTATQHTSFSRLTVDNRRWGNVEHDAELVARVH
                     L"
     assembly_gap    765..774
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <775..1691
                     /locus_tag="PLV68_16750"
     CDS             <775..1691
                     /locus_tag="PLV68_16750"
                     /inference="COORDINATES: protein motif:HMM:NF014801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HRE02951.1"
                     /translation="PSKLARLVPMGRPRGVVTAAAAEHLGVSAQAVVADATIDSMTSA
                     VGTGAIDQTRCGLVIGTTAVLATHLPDRRADVEHGLMAAPSPIPGHYVVVAENGIGGK
                     ALETFVHGVVYADDGLTGASPSDAYERVLAAAAGVPAGANGVVYLPWLVGSMAPSYHS
                     RVRGGFLGLDLDTTRADMARAVLEGVAANAAWLLPAVATLAGTSSPDIGFGGGVAVSP
                     LWAQILADTMQLPIRRMANPRATNAHGAALLALVHAGELALTDLPTVIAGYTHEPDPA
                     LADWAVAQVARLAAAHTHLATLATTHPT"
     gene            1766..>2568
                     /locus_tag="PLV68_16755"
     CDS             1766..>2568
                     /locus_tag="PLV68_16755"
                     /inference="COORDINATES: protein motif:HMM:NF014708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HRE02952.1"
                     /translation="MSGTSSGREDPGQVGDGATAVEVVDGRAAEVGGFAVARVLPLGH
                     RRRTVGAWCFVDLMGPGVTTADHGLDVGPHPHIGLQTVTWLLDGAVLHRDSLGSEQVI
                     HPGQLNLMTAGAGIAHSEETTGTHTGALHGVQLWVAQPAETRNGPPAFEHHAELPVVG
                     LANGAATVLAGTLMGVTSPARGDTSHVGADLDLRAGRTVVALDPRFEYAVVPLTGPVA
                     VGGGSGGSGDVARPGQLAYLGPGRGANLVDALDMVVMMMRDQDARQPPAA"
BASE COUNT          350 a          893 c          922 g          393 t
ORIGIN      
        1 acttgcggtt gagcgcttcc tgcagggtga gttccgggta gcccagtccg gtgatgcccg
       61 tgacatccgt actcgccatc gcccccgacc cagccggcgg caaggcgccc gagccgcggc
      121 aggcgcgcct ggtgtggacc gtggtggtgc tgtcgtgacg tgggtgcttg ccatcgacct
      181 gggcacgacg ggtgtcaagg tggccgtggt cgacgctgag ggtttggcgc tgggcgccgc
      241 cggtgagacg ctgccgttgc tgagtgttgc ggatggtggg gtcgagcagg acgccgacgg
      301 ctggtggcag gcgatcggcc gatgtgcccg tcgggtgctg gtgtcggtgc cagcggcgcg
      361 cgccgaggtc gggttgatcg cggtgaccag ccagtacaac tcgacggtcg cggtcgatgc
      421 ccgcgggctg ccggtggcca acgcggtgat gtgaatggac ggccgcggcc gtcgcaacca
      481 cccgggcgtg gcccgcggtg ccgatcgtgg cgactggatc gagcgccacg gcatggcccc
      541 gaactacggc gacacggcgg gccacgtcgc gaccatccgc gcggtgtccc ccgatgccta
      601 tgccgctgcc catgccttcg tcgagccgat ggatgcgatc gcggcccgcc tgaccggcca
      661 cgtcaccgca acgcagcaca cgtcgttttc gcggttgacg gtcgacaatc ggcgctgggg
      721 caacgtcgag cacgacgccg agttggtggc gcgggtgcat ctcgnnnnnn nnnnatccgt
      781 ccaagctggc gcggctggtg ccgatgggta gaccccgcgg tgtcgtcacc gcagcagcag
      841 cggaacacct cggtgtctcg gcccaggcag tcgttgcaga tgcaacgatc gactcgatga
      901 cgtctgccgt cggcacggga gcgatcgacc agacccgctg cggactggtg atcggcacga
      961 ccgccgtgct ggccacacac ctgccggacc gccgcgccga cgtcgagcac ggcctgatgg
     1021 ccgcaccgag tcccatcccc ggccactacg tcgtggtcgc ggagaacggc atcggtggca
     1081 aggcgctgga gacgttcgtc catggcgtcg tctatgccga cgacggtctg acgggtgcat
     1141 ctccctccga cgcctacgag cgagtgctcg ccgccgcggc gggcgtaccc gctggtgcca
     1201 acggtgtggt ctacctgccc tggttggtcg gctcgatggc cccgtcgtac cacagccggg
     1261 tgcgcggcgg cttcctcggc ctcgacctcg acaccacgcg cgccgacatg gcccgcgcgg
     1321 tgctggaagg cgtagccgcc aacgctgcct ggctcctccc cgccgttgcc accttggccg
     1381 gcacgagttc tcccgacatc gggttcggtg gcggcgtggc ggtgtcaccg ctgtgggcgc
     1441 agatcctggc cgacacgatg cagctgccga tccgccgcat ggccaacccg cgcgccacca
     1501 acgcccacgg cgcggcgctg ctcgccctcg tccacgcggg agagctcgcg ctgaccgacc
     1561 tcccgacggt gatcgccggg tacacgcatg aacccgaccc ggccctggcc gactgggctg
     1621 tggcccaggt ggcccgtctg gccgccgccc acacccacct cgccaccctc gccaccaccc
     1681 acccgacctg acccggccgc tgtcgacgcc tgcagacgcg ccgaggagat cgtcaacgac
     1741 atcggccgag gggcagactg aagccatgag cggtacgagt tctgggcgcg aagaccctgg
     1801 ccaggttggt gacggtgcga ctgcggtcga ggtggtcgat ggacgggcgg cggaggtcgg
     1861 tgggttcgcc gtggcccggg tgttgccgct ggggcatcgt cgccgcacgg tcggtgcgtg
     1921 gtgcttcgtc gatctgatgg gtcccggggt gacgactgcc gatcacggtc tcgacgtggg
     1981 gccgcacccg cacatcggtc tgcagacggt gacctggctg ctcgacggcg cggtcctgca
     2041 ccgcgacagc ctcgggtccg agcaggtgat ccaccccggt cagctcaacc tgatgaccgc
     2101 gggtgctggc atcgcccact ccgaagagac gaccggaacg cacacgggtg cgctgcacgg
     2161 ggtgcagttg tgggtcgccc agccagcaga gacgcgcaac ggtccgcctg ccttcgaaca
     2221 ccacgccgaa ctgccggtgg tcgggctcgc caacggtgcc gcgacggtgc tcgccggaac
     2281 gctgatgggg gtcacgtcac cggcgcgggg cgacaccagc cacgtcggcg ccgacctcga
     2341 cctgcgcgct gggcgcaccg tcgtcgcact cgatccacgg ttcgaatacg cagtcgtccc
     2401 gctgaccggc ccggtcgccg ttggtggcgg tagcggcggt agcggcgacg ttgcccggcc
     2461 ggggcagttg gcgtacctcg gccccggtcg cggcgcgaat ctcgtcgacg ccctggatat
     2521 ggtcgtcatg atgatgcgtg atcaggatgc gcgacagccc ccagccgc
//
LOCUS       DAORNF010000501         2565 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_30456_length_2565_cov_1.522709, whole genome shotgun sequence.
ACCESSION   DAORNF010000501 DAORNF010000000
VERSION     DAORNF010000501.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30456_length_2565_cov_1.522709"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            70..>389
                     /locus_tag="PLV68_16760"
     CDS             70..>389
                     /locus_tag="PLV68_16760"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HRE02953.1"
                     /translation="MATKVAGVHGTNHPELHEVARLTSELKADLEPHLRKEETVLFPM
                     IRRLGETAASDQPETLVFHCGSLQNPISVMLMEHDRTGELLQELRAASHDYALPGDAC
                     GSYQA"
     assembly_gap    390..473
                     /estimated_length=84
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            500..>851
                     /locus_tag="PLV68_16765"
     CDS             500..>851
                     /locus_tag="PLV68_16765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HRE02954.1"
                     /translation="MTPPAGTGDTVRLRRAYDPAGVGDGYRVLVDRLWPRGVRREALH
                     LDEWCTNIAPSAELRAWFGHDAAKWTEFQTRYRRELDGNPDVARLAGIAAHATLTLVY
                     GAHDTEHNQAVVIRD"
     assembly_gap    852..1016
                     /estimated_length=165
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1017..>1468)
                     /locus_tag="PLV68_16770"
     CDS             complement(<1017..>1468)
                     /locus_tag="PLV68_16770"
                     /inference="COORDINATES: protein motif:HMM:NF014961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HRE02955.1"
                     /translation="RRDGKPNLSISDFVAPAGIGVVDHVGAFAVTCGDEEVAIAERFE
                     AANDDYSSIVLKALADRFAEALAEWTHERVRTSLWGYAPDEHLTCDLLVAEAYRGIRP
                     APGYPAQPDHSEKATIFELLDAERLAGVRLTESYAMWPSAAVAGWYFA"
     assembly_gap    1469..1565
                     /estimated_length=97
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1566..>1890)
                     /locus_tag="PLV68_16775"
     CDS             complement(<1566..>1890)
                     /locus_tag="PLV68_16775"
                     /inference="COORDINATES: protein motif:HMM:NF014961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HRE02956.1"
                     /translation="IDSEYARVVEAHQRAEVERQRLTIEAARANAGDFRFDASTVVAP
                     SYLGARTVEVADLATLAEYIDWTPFFHAWGLRGRFPAILDDAELGEAARPLWDDARQM
                     LDRIVA"
     assembly_gap    1891..1926
                     /estimated_length=36
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1927..>2195)
                     /locus_tag="PLV68_16780"
     CDS             complement(<1927..>2195)
                     /locus_tag="PLV68_16780"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HRE02957.1"
                     /translation="LGVMVHAATILDTARAEGADIIGLSGLITPSLDEMVNVANEMQR
                     QGFSTPLLVGGATTSRIHTALRITPAYSSPVVHVADASRAAGVIS"
     assembly_gap    2196..2300
                     /estimated_length=105
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2301..>2565)
                     /locus_tag="PLV68_16785"
     CDS             complement(<2301..>2565)
                     /locus_tag="PLV68_16785"
                     /inference="COORDINATES: protein motif:HMM:NF014646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="B12-binding domain-containing protein"
                     /protein_id="HRE02958.1"
                     /translation="VAERLTHALVNGITEFIDADVEEARAASVSPVAVIEGPLMDGMN
                     VVGDLFGAGKMFLPQVVKSARVMKQAVGYLMPFLEANGGGGLRN"
BASE COUNT          346 a          722 c          699 g          311 t
ORIGIN      
        1 cagttcgtcg accaccgcga agccacccat cacgcctacc tccacggcga gctgccccgt
       61 ctcgaggctc tggcgaccaa ggtggccggc gtccacggga ccaatcatcc ggaactgcac
      121 gaggtggctc gcctgaccag cgaactcaag gccgacttgg agccacatct gcgcaaggag
      181 gagacggtgc tgttcccgat gatccgccgc ctcggcgaga cggctgcgag cgaccaaccc
      241 gagacgttgg tgttccactg cggttcgttg cagaacccga tctcggtgat gctgatggaa
      301 cacgaccgca ccggtgagct gctgcaagaa ctgcgtgccg cctcgcatga ctacgccctg
      361 ccgggcgacg catgcggcag ctaccaggcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngttctcg
      481 ccgccgaggc agccctgggg tgacaccacc cgccggcacc ggcgacaccg tacggttgcg
      541 acgcgcgtac gacccagcgg gagtcggcga cggctaccgg gtgctggtcg accgtctgtg
      601 gcctcgcggt gttcgccggg aggcgctcca cctcgacgaa tggtgcacga acatcgctcc
      661 gagcgccgag ttgcgcgcgt ggttcggcca cgacgcagcc aagtggaccg agttccagac
      721 tcgctatcgc cgtgagctcg acggcaaccc cgatgtcgcc cgcctggccg gcatcgccgc
      781 ccacgccacg ttgaccctcg tctacggcgc gcacgacacc gagcacaatc aggcggtcgt
      841 catccgcgac gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnagcg
     1021 aagtaccagc cggcgacggc cgccgaaggc cacatcgcgt agctctcggt gaggcgcacc
     1081 ccggcgaggc gttcggcatc gagcagttcg aaaatggtcg ccttctcgct gtgatcgggc
     1141 tgggccggat agccaggtgc cggacggatg ccgcggtagg cctcggcgac cagcaggtcg
     1201 caggtcaaat gctcgtcggg ggcgtagccc cacaacgacg tgcgcacacg ttcatgggtc
     1261 cactcggcga gtgcctcggc gaaccggtcg gccagcgcct tgagcacgat cgaggagtag
     1321 tcgtcgtttg ccgcctcgaa ccgttctgcg atggcgacct cctcgtcgcc gcaggtgacc
     1381 gcgaacgcgc cgacgtgatc gacgacgccg atccctgccg gcgcgacgaa gtcggagatc
     1441 gacaggttcg gcttgccatc gcggcgggnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnngcgac gatgcggtcg agcatctggc gcgcatcgtc ccacaacggg cgtgcggcct
     1621 cgccgagttc ggcgtcgtcg aggatcgccg ggaaccggcc ccgcagaccc caggcgtgga
     1681 agaacggggt ccagtcgatg tactcggcca acgtggccag gtccgcgacc tcgaccgtgc
     1741 gagcgccgag atacgacggc gcgacgacgg tcgaggcgtc gaagcggaag tcacccgcgt
     1801 tggctcgcgc ggcctcgatg gtgaggcgtt gacgttcgac ctcggcccgc tggtgcgctt
     1861 cgacgacccg cgcgtattcg gagtcgatct nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnntgga gatcacgccc gccgcgcgtg acgcgtcggc aacatggacc acgggcgagg
     1981 agtacgcagg ggtgatccgc agcgcggtgt ggattcggct ggtggtcgca ccgccgacca
     2041 acaacggcgt gctgaagccc tgacgctgca tctcgttggc gacgttgacc atctcgtcga
     2101 gcgacggcgt gatcaggccg gacagcccga tgatgtcggc accctcggcg cgagcggtgt
     2161 cgaggatggt ggcggcgtgc accatcaccc ccagannnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn gttgcgcagc ccgcccccac cgttggcctc caggaacggc
     2341 atcaggtagc cgaccgcctg cttcatcacc cgggccgact tgaccacttg cggcaggaac
     2401 atcttgccgg caccgaacag gtcgccgacc acgttcatcc cgtccatcag cgggccttcg
     2461 atcacggcca ccgggctgac cgacgcggca cgcgcctctt cgacgtcggc gtcgatgaac
     2521 tcggtgatgc cgttgaccag cgcgtgggtc aaccgctcgg ccacc
//
LOCUS       DAORNF010000502         2560 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_30524_length_2560_cov_1.698603, whole genome shotgun sequence.
ACCESSION   DAORNF010000502 DAORNF010000000
VERSION     DAORNF010000502.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30524_length_2560_cov_1.698603"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..760
                     /locus_tag="PLV68_16790"
     CDS             <1..760
                     /locus_tag="PLV68_16790"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HRE02959.1"
                     /translation="TRHRFSAWFRARPKVRIGNRQARVALVPTCVVEFTAPAIGHDLV
                     RVYERNGIEVALADVGCCGAPLLQRGDTLADQVRKGDDIVVAQPTCALTVRRDYLAHV
                     PGADTEAVAAHTYDASEYLMRQHRSGESLLDTDFVGPLPRSIVYHAPCHIRAQRVGLK
                     GRDLLRLTGATVSVVQQCSGIDGVGGLRASADELAGPLAARLAEELERRPAATIVGDC
                     HLSNVAIAGATGRVPSHPLQVLARAYGFAPEPEA"
     gene            complement(779..>1289)
                     /locus_tag="PLV68_16795"
     CDS             complement(779..>1289)
                     /locus_tag="PLV68_16795"
                     /inference="COORDINATES: protein motif:HMM:NF020133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02960.1"
                     /translation="HADNAAPALLGGVVASADDRSLVVPLRLDPAIVVWIPSFATRTD
                     QSRAALPATVSRADAVFNIGRTALLVAALAAGDPSELAHATADRLHQPVRFAAAPASH
                     EAYQAAIAAGAWCAWLSGSGPTVAAMCALDRADHIAAQIAARLPDGGHTKVLRIDHEG
                     ASLTAQQPA"
     assembly_gap    1290..1304
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1305..>1543)
                     /locus_tag="PLV68_16800"
     CDS             complement(<1305..>1543)
                     /locus_tag="PLV68_16800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02961.1"
                     /translation="VDHDPAPPNARLLDDHALATVAFRRLGGRGRLWEASPIPMARGL
                     GYSAAVRVGGLLVACAQRHPDGSDGDVIITVDGDEV"
     assembly_gap    1544..1642
                     /estimated_length=99
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1643..>2560)
                     /gene="trpD"
                     /locus_tag="PLV68_16805"
     CDS             complement(1643..>2560)
                     /gene="trpD"
                     /locus_tag="PLV68_16805"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HRE02962.1"
                     /translation="FVVALRAKGETGEELSGLLDAVLAAASIVPLDDAVRDRAVDIVG
                     TGGDRSHSINVSTMSAIVVAGAGVTVCKHGARGSSSKSGAADVLEALGVAISLDAEGV
                     RRCIDETGIGFCLATTFHPAFRFAGPSRREIGIPTVFNLLGPMANPGRVRRQVIGVAN
                     PAVAQRMLASLRIHGSIKAWVVHGSGLDELTTTGPSTVLSLDDGEVTTFEIDPVSLGL
                     TPAIAEELTGGDAAENAEVVRRVLAGAHGPHRDIVVLNAAAGLVVAGVAGDIREGLSL
                     AAASIDSGKALDVLDRFIAASQRIAASTA"
BASE COUNT          364 a          862 c          886 g          334 t
ORIGIN      
        1 gacccgccac cgcttcagtg cctggttccg ggccaggccc aaggtccgca tcggcaaccg
       61 ccaggcgcgc gtggcgctgg tgccgacctg cgtcgtcgag ttcaccgcgc ccgcgatcgg
      121 tcacgacctg gtgcgcgtct acgagcgcaa cggcatcgag gtcgctttgg ccgacgtcgg
      181 ctgctgcggt gccccgctgc tgcagcgcgg cgacacgctg gccgatcagg tgcgcaaggg
      241 tgacgacatc gtcgtggccc agccgacatg cgcgctcacc gtgcgacgcg attacctggc
      301 ccacgtgccg ggagccgaca ccgaagcggt cgccgcgcac acctacgacg cgagcgagta
      361 cctgatgcgc cagcaccgct cgggtgagtc gctgctcgac accgacttcg tggggccgtt
      421 gccgcgcagc atcgtgtacc acgcgccctg ccatatccgt gctcaacggg tcggattgaa
      481 gggccgcgat ctgcttcgtc tgaccggtgc caccgtcagc gtcgtccagc agtgcagcgg
      541 tatcgacggt gtaggcggtc tgcgggcgag cgccgacgag ctggccggtc cgctcgcggc
      601 gcggctcgcg gaagagctcg aacgccgccc cgcagccacc atcgtcggcg actgccacct
      661 gtccaacgtg gccatcgccg gggcgacggg ccgagtgccg agccatccgc tgcaggtgct
      721 ggcacgggcc tacgggttcg cccctgagcc cgaggcctag tccggcgtcg cggcagacct
      781 aggcgggctg ctgcgcggtc agagacgcgc cctcgtggtc gatgcgcagc accttggtgt
      841 ggcccccgtc gggaagcctt gcggcgatct gagccgcgat gtggtcggcg cggtcgagcg
      901 cgcacatggc ggcgacggtg gggccagacc cagacagcca cgcgcaccac gcacctgctg
      961 cgatggccgc ctggtatgcc tcgtgcgagg cgggcgccgc ggcgaagcga accggctgat
     1021 ggaggcggtc ggcagtggca tgggccaact cggatgggtc accggcagcg agcgcggcca
     1081 ccaacagcgc cgtgcgaccg atgttgaaca ccgcgtcggc gcgtgacacg gtcgcgggca
     1141 gtgccgcgcg gctttgatcg gtgcgggtcg cgaacgacgg gatccacacg acgatcgccg
     1201 ggtcgagccg cagcggcacg accagcgagc ggtcgtccgc gctggccacg acaccgccga
     1261 gcagggccgg tgcggcgttg tcggcatggn nnnnnnnnnn nnnnacttcg tcgccgtcga
     1321 cggtgatgat cacatcgccg tcggaaccgt cgggatggcg ctgcgcacag gccacgagca
     1381 ggccgccgac gcggaccgcg gcgctgtagc ccagtccgcg ggccatcggg atcggcgacg
     1441 cctcccacaa ccttcctcgg ccaccgaggc gccggaaggc gacggtggcc agcgcatggt
     1501 cgtcgagcag gcgggcgttc ggcggggcgg ggtcgtggtc gacnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nntcaggcgg tgctcgccgc gatgcgctgc gaggctgcga
     1681 tgaagcgatc gagcacgtcg agcgccttac cactgtcgat cgatgccgcg gccaacgaca
     1741 ggccctcgcg gatgtcaccg gcgactccgg caacaaccag tccggccgcg gcgttgagca
     1801 caacgatgtc gcgatgtggg ccgtgagcgc ccgccagcac gcggcggacg acctcggcgt
     1861 tctcggcggc gtcaccaccc gtgagctctt cggcgatggc gggggtgagg ccgagagaga
     1921 ccgggtctat ctcgaaggtg gtgacctcgc cgtcgtcgag cgacaacacg gtcgacggcc
     1981 cggtcgtggt cagctcgtcg agtccgctgc cgtgcaccac ccaggccttg atcgaaccgt
     2041 ggatgcgcag cgacgccaac atccgctggg cgactgccgg gttggcgaca ccgatcacct
     2101 gacggcggac gcggccgggg ttggccatcg gtccgagcag gttgaacacc gtggggatgc
     2161 cgatctcgcg ccggctcgga ccggcgaagc ggaacgccgg gtggaacgtg gtcgccaggc
     2221 agaagccgat gccggtctcg tcgatgcagc gccgcacgcc ctcggcgtcg aggctgatcg
     2281 ccacgcccag cgcttccagc acgtcggccg ccccgctctt ggacgacgaa ccgcgggcgc
     2341 cgtgcttgca caccgtgacg cccgccccgg cgaccacgat cgcggacatg gtcgagacgt
     2401 tgatcgagtg actgcgatcg ccgccggtac ccacgatgtc gaccgcgcgg tcgcgcaccg
     2461 cgtcgtcgag cggcacgatc gaggctgcgg ccagcaccgc gtcgagcagc cccgacagct
     2521 cctcgcccgt ttcacccttg gcccgcaggg caacgacgaa
//
LOCUS       DAORNF010000503         2557 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_30578_length_2557_cov_1.529177, whole genome shotgun sequence.
ACCESSION   DAORNF010000503 DAORNF010000000
VERSION     DAORNF010000503.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2557
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30578_length_2557_cov_1.529177"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>561
                     /locus_tag="PLV68_16810"
     CDS             <1..>561
                     /locus_tag="PLV68_16810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRE02963.1"
                     /translation="VMGNCGVTFAPVRPGDQTYLATMMESVEDIPAAAILSGLPWTWS
                     TYGEFLAELAKRPLGPNAGGLVGHCALRYYAMGEDSLGSEPASELSQSTMERLVREAL
                     DGGALGFSTSRTTLHIVPDGRPVPGTFAPASELYALGGVLRDAGKGVLGAVTRLHEET
                     GSSIGETLAEIDLLGELALAIGRPVTF"
     assembly_gap    562..624
                     /estimated_length=63
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <625..>848
                     /locus_tag="PLV68_16815"
     CDS             <625..>848
                     /locus_tag="PLV68_16815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02964.1"
                     /translation="GAQLWPQTTVRPIGWLYGLAHRTPFDKNPSWRSLRSQPLEAKLA
                     VLADATARAELIDEAARNPPAVDLSRVYAF"
     assembly_gap    849..1042
                     /estimated_length=194
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1043..1446
                     /locus_tag="PLV68_16820"
     CDS             <1043..1446
                     /locus_tag="PLV68_16820"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRE02965.1"
                     /translation="REVVRDELAPLADAGAHVGQIMDASQPSYVLAYWMRERGVLGLA
                     DAVRRLTSVPAKVFGIADRGVIAPGAYADINVIDIGAVELDHPEFVHDLPGGAGRFVQ
                     RASGYRWTLVNGAPVLADGARTGALPGHMLR"
     gene            complement(1495..>2557)
                     /locus_tag="PLV68_16825"
     CDS             complement(1495..>2557)
                     /locus_tag="PLV68_16825"
                     /inference="COORDINATES: protein motif:HMM:NF018938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HRE02966.1"
                     /translation="FPIAEVCDDGSAVITKQPGTGGIVTVGTVTAQLLYEIDQPAYAN
                     PDVVARFDMIALTQEGPDRVRISGTRGEPAPRQLKVALNSIGGWRNTMTMVITGLDIE
                     AKAERAIAMLGEVLGGFDQFAEHFIELVRGDRPDAPSNSLATAHLRITVKDPDRDKVD
                     RRFSGAIMELLLASYGGAFSATPPTAATEFGIYRPAYVDAEQVRHVVTLPDGSDLVVA
                     HPEPVAARAITVETPPRRAVAGGPTQRVPLGRVCGARSGDKGGNANVGLWARSDATYA
                     WLRSALTVDLFRALLPEADELIVRRYELPNLRALNFVVEGILGAGVASSVRFDAQAKG
                     LGEYVRSRLVDIPTAVLNA"
BASE COUNT          351 a          760 c          805 g          384 t
ORIGIN      
        1 gtgatgggca actgcggcgt cacctttgcg ccggtgcgtc cgggtgacca gacgtacctg
       61 gcgacgatga tggagtcggt ggaggatatc ccggcagccg cgattctcag tggtctgccc
      121 tggacgtggt cgacctacgg agagttccta gccgagctcg cgaagcgtcc actcggcccg
      181 aatgccggag gtctggttgg gcactgcgcg ctgcggtact acgcgatggg cgaagacagc
      241 cttggcagcg agcccgcctc tgagttgtcg cagtcgacga tggagaggtt ggtccgcgag
      301 gccctcgatg gcggcgccct cggattctcg acgtcgcgca ccacactgca catcgttccg
      361 gacggccgcc ccgttcccgg cacgttcgcg ccggcgagcg agttgtacgc gctcggcggc
      421 gtcttgcgcg atgcgggcaa gggtgtgctg ggtgccgtga cccgcctgca cgaggagacc
      481 ggatcgtcga tcggcgagac cttggccgag attgacctgc tcggtgagct ggcgctggcc
      541 atcgggcggc cggtcacgtt cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      601 nnnnnnnnnn nnnnnnnnnn nnnngggcgc tcaactgtgg ccgcagacca cggtacgccc
      661 gatcggctgg ctctacggct tggcccaccg cacgccgttc gacaagaacc cgtcgtggcg
      721 atcgctccgt tcgcagccct tggaggccaa gttggctgtc ctggccgatg ccacggcgag
      781 ggctgagctg atcgacgaag cggcgcgcaa cccgccggcg gtcgacctga gccgggttta
      841 cgccttccnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1021 nnnnnnnnnn nnnnnnnnnn nnagcgtgag gtagtgcgcg acgagcttgc gcccctcgcc
     1081 gacgcgggcg cgcacgtcgg acagatcatg gatgcaagcc agccgtcgta cgtgctcgcc
     1141 tactggatgc gcgagcgcgg cgtgctgggt cttgccgacg ccgtccggcg cctgacgtcg
     1201 gtgcccgcca aggtgttcgg catcgcggat cgcggtgtca tcgccccagg cgcgtacgcc
     1261 gacatcaacg tcatcgacat cggcgccgtc gagttggatc atcccgagtt cgtccacgac
     1321 cttcccggtg gcgcggggcg cttcgtccag cgagccagcg gctatcgctg gacgcttgtc
     1381 aacggcgctc ccgtactcgc cgatggtgcg cgtaccggtg cccttcccgg ccacatgctt
     1441 cgctaagtca gcgcaacggg tggacggcac ccgtgccgat cggagagcgt cggctcaggc
     1501 gttcaacacc gccgtcggga tgtcgaccag gcgcgagcgg acgtactcgc cgaggccttt
     1561 ggcctgggcg tcgaagcgca ccgacgacgc caccccggca ccgaggatgc cttcgaccac
     1621 gaagttgagc gcccgcaagt tggggagctc gtagcggcgg acgatgagct cgtcggcctc
     1681 tggcagcaac gcacggaaca ggtcgaccgt cagcgccgag cgaagccagg cgtaggtcgc
     1741 gtccgatcgg gcccacaggc ccacgttggc attgccgccc ttgtcgccgg atcgggcgcc
     1801 gcagacccgt ccgagcggga cccgctgtgt cgggccgcca gcgacggcgc gacgaggtgg
     1861 cgtctcgacg gtgatcgctc gagcggcgac tggctccgga tgggcgacga ccagatcgct
     1921 gccgtcaggc agcgtgacga cgtgacgaac ctgttccgcg tcgacgtatg ccggacggta
     1981 gatgccgaac tcggtggccg cggtcggcgg cgtcgcggag aacgcgccac cgtacgaggc
     2041 caacaacagt tccatgatcg ccccggaaaa gcggcgatcg accttgtccc ggtcagggtc
     2101 cttgacggtg atccgcagat gggcggtggc cagtgagttc gacggggcat cggggcgatc
     2161 accgcggacg agttcgatga agtgctcggc gaactggtcg aagccgccga gtacctcgcc
     2221 aagcatcgcg atcgcccgct cggccttggc ctcgatgtcg agcccggtga tgaccatcgt
     2281 catcgtgttc cgccagccac cgatcgagtt cagcgcgacc ttcaactggc gcggtgctgg
     2341 ctcgcctctg gtgccgctga tccgcactct gtccggtcct tcctgagtca gcgcgatcat
     2401 gtcgaagcgg gcgacgacat cggggttggc gtatgccggc tgatcgatct cgtagagcag
     2461 ttgggcagtg accgtgccga cggtgacgat gccgccggta cccggctgct tggtgatcac
     2521 agccgatccg tcgtcacaga cctccgcgat cggaaac
//
LOCUS       DAORNF010000504         2554 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_30631_length_2554_cov_1.510604, whole genome shotgun sequence.
ACCESSION   DAORNF010000504 DAORNF010000000
VERSION     DAORNF010000504.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30631_length_2554_cov_1.510604"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            177..>541
                     /locus_tag="PLV68_16830"
     CDS             177..>541
                     /locus_tag="PLV68_16830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HRE02967.1"
                     /translation="MLSARKNMDVVGAYARLGTYRAAGEACGVDPKTVKRKVEAHAKG
                     LLDDDRAERAPVAKNTDGVRAVVLEELRETGGRGTAKRLLPIARKAGYAGSDRNFRRL
                     VAGVKRQVRAEVGRHQRRPA"
     assembly_gap    542..551
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            584..1690
                     /locus_tag="PLV68_16835"
                     /pseudo
     CDS             584..1690
                     /locus_tag="PLV68_16835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611569.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            1687..2448
                     /gene="istB"
                     /locus_tag="PLV68_16840"
     CDS             1687..2448
                     /gene="istB"
                     /locus_tag="PLV68_16840"
                     /inference="COORDINATES: protein motif:HMM:NF013828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HRE02968.1"
                     /translation="MSRQPAPLAPDVEAALRRLRLAAIRREAPDVLATAKAQRWTPEE
                     VLRVLVEAEISSRDASNTRNRMTAARFPIDKTLDEFRLDQSSIPRSSHDYLVTLEWIR
                     QAENLCLVGPAGTGKTHYLIGLGHAAVTAGHKVRYFTAVDLVEHLYRAVADNTVGKAI
                     ETVFRNDLIIIDEIGFAPLDHTGCQLLFRLVAAAYERRSIAIGSHSPFDQWGRFLPDQ
                     PTAVSLLDRLCHHAHIITTTGDSYRLTHRTTRKEA"
BASE COUNT          448 a          886 c          803 g          407 t
ORIGIN      
        1 tcaccggcac ccgggaccga ttcccagttg tccatggcca tgtcaaagtc ctcgctggtg
       61 gccaggaaaa gtcctcgcgt ggtgagtagg tagatccact ttgcgggccg cctcgagcgg
      121 tgatcgtgaa cgacgaccaa gtcagttcag atcacacgct cgaggggttg gcccaattgt
      181 tgtcagcgag gaagaacatg gatgttgttg gcgcgtacgc gcgtctgggg acctatcgcg
      241 ctgctggtga ggcgtgcggt gtggacccga agaccgtcaa acgtaaggtc gaagctcacg
      301 cgaagggtct gttggatgac gaccgagcag agcgggcgcc ggtggcgaag aacactgacg
      361 gcgtgcgtgc cgtggtgctc gaggaactcc gcgagaccgg cggccgggga acggcgaagc
      421 ggttgctgcc gatcgctcgc aaggccgggt acgcgggttc ggaccggaac ttccggcgcc
      481 tggtcgccgg cgtgaaacga caggttcgtg ccgaggtcgg ccgtcatcag cgacgcccgg
      541 cnnnnnnnnn naggtggcgt agccgggggc gtcgaccttg acgatgtggg tgccgggcga
      601 gacgttggtg atcgattggg ggctgcaccc gtcaggggtg aaggtgttct gcgcggtgct
      661 cgcctggtcg aggttccggt tcgtcagggt cgctcgggac gagaccgcag caacgacgat
      721 ggcgttgctc gccgactgct tcgacatgct cggcggtgtc ccgggcaagg tgctcgctga
      781 tcggatgggc tgcctgaagg gtggcacggt cgcgaacgtg atgatcccga ccccggacta
      841 cgtccggttc gcgacccact accggttcgt cccggacttc tgccaccccg ccgatcccga
      901 gtcgaaaggg atcgtcgaga acctcgtcgg ctacgcgaaa cgcgacctgg tcctgcccga
      961 ctctgacgac ctcgccgtgt tgaacgacgc ctgccgtgac tggtgttcag aggtcaacgc
     1021 ccgtgaacac tccgagatct gcgctgtccc cgatgtccgg ctcgccgagg aacgcgagtt
     1081 gctgcgcgag ttgccgatgc tgcgcccgcg gatcggccga gtcgaggtcc gcaaggtcga
     1141 caagctctcc acgatccgtg tcgcctccgc ccgctattcg gtcccgcacc ggctcgtcgg
     1201 cacacaggtc gaagcggtca ccttcgacgg caaggtcagg atctacaccc ttgacggcga
     1261 cctcgtcgct gaccaccgcc agctcgcacc cggcgaagca gccgtgctcg atgagcacta
     1321 cccgacacca cgtcgggcgc cgtcacgcgg gccgagagcg cgcagcggga tcgagcgtga
     1381 gttcctcgcg ttgggtgaac cggccgaggt gttcatccgc caaggcgccg cggcggggat
     1441 gacgatgctg ccgaaagaga tcggcgagat cgttgacgac atcctgcccg cgcacggccc
     1501 agagctggtc gccaaagcgg tcgctcgggc ggcacgtttc ggcaggttcc gtgccgcgga
     1561 tgtccgctcg atcctcgcga tcggcaccgc cctgcccgaa cccgctgcgg ccggcgacgc
     1621 ggtcgtcgtt gcgctcccga cggctgaggt ccgctcgttc gacgcgtacg cgatggagaa
     1681 cctcgcatga gtcgccaacc cgccccgctc gcacccgatg tcgaagcagc gctccgcaga
     1741 ctgcggctcg ctgcgatccg acgcgaagca cccgacgtgc tcgccaccgc gaaggcccaa
     1801 cgctggacac ccgaagaagt cctccgcgtg ctcgtcgaag cagagatcag ctcacgcgac
     1861 gcgtcgaaca cccgaaaccg gatgaccgcc gcacgcttcc cgatcgacaa gaccctcgac
     1921 gagttccgcc tcgaccagtc ctcgatcccg cgctcatcac acgactacct cgtcacactc
     1981 gagtggatcc gacaagccga gaacctctgc ctcgtcggcc ccgcaggcac cggcaaaacc
     2041 cactacctca tcggcctcgg acacgccgcc gtcaccgccg gccacaaggt ccgctacttc
     2101 accgctgtcg atctcgtcga gcacctctac cgcgccgtcg ccgacaacac cgtcggcaaa
     2161 gccatcgaga ccgtgttccg caacgacctc atcatcatcg acgagatcgg cttcgcaccc
     2221 ctcgatcaca ccggctgcca gttgctgttc cgactcgtcg ccgccgccta cgaacgacgc
     2281 tccatcgcca tcggctcaca cagcccattc gaccaatggg gccgcttcct gcccgaccag
     2341 cccaccgccg tcagcctgct cgaccggctc tgccaccacg cccacatcat caccaccacc
     2401 ggcgacagct accgactcac ccaccgcacc acccgcaagg aggcataacc gcgaggaatt
     2461 ttgttggcca ccagcgagga ccctggttgg ccaccagcga ggacatcaac ttggccattg
     2521 acaggtgttg agcggaacgg gagggggtcc gcct
//
LOCUS       DAORNF010000505         2551 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_30678_length_2551_cov_1.687901, whole genome shotgun sequence.
ACCESSION   DAORNF010000505 DAORNF010000000
VERSION     DAORNF010000505.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30678_length_2551_cov_1.687901"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>217
                     /locus_tag="PLV68_16845"
     CDS             <1..>217
                     /locus_tag="PLV68_16845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02969.1"
                     /translation="ATRIAQTVANHAIVPVSPRGFDVFAQNPTHAIADVSGYYLGTPA
                     PSPFGGPQNVDPTPVFCHGFTSEVIQLA"
     assembly_gap    218..282
                     /estimated_length=65
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            327..>676
                     /locus_tag="PLV68_16850"
     CDS             327..>676
                     /locus_tag="PLV68_16850"
                     /inference="COORDINATES: protein motif:HMM:NF013625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02970.1"
                     /translation="MAYQKYYRMSASGRIDAATATKLSYPNCKPTAGTNSGTLMEIDK
                     GKQLGFFIRNGQLLWVINVSTGGNYFYEDEINGKKYLDRAYTDVGNFSVYRVSDKVRY
                     EGTLGTMYRPRFVIR"
     assembly_gap    677..808
                     /estimated_length=132
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(977..2203)
                     /locus_tag="PLV68_16855"
     CDS             complement(977..2203)
                     /locus_tag="PLV68_16855"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRE02971.1"
                     /translation="MRRRLVRNTILVSMMVLAVLATPVVLLLKRATEAELQGRLDAHA
                     DSIVAVIDEDVRAHRAIDVALLITMVPHDTRVVVSLNGVVDVQFGPQADTARHGYGVA
                     VSGRVVDVSTDDSELRDRLTRQFMLLGVLAAGGVLLAAVLAAIFGARLTRPLEQLAAS
                     AGRLGAGDFSAALPPPSGIREIDDIRSTLSASASRLDQTLIAERSFTGDATHQLRTGL
                     TGIALQLELLAAMHPDVHDDVSHALSQTDRMTATIDELPWAMLAKGPARGGRGGARMT
                     VQLRDIASEHRGDWLQRYSQRKRLLYCSGSGSPVIATPGFVGQIIDILLDNALTHGQG
                     VVTIHVSDRQLRVRDEGRIDAETAKGLFRGSDDPAAPHGRGLSLARRLAQADGGRLEL
                     TERSPTTLVVTYPAAV"
     gene            complement(2225..2497)
                     /locus_tag="PLV68_16860"
     CDS             complement(2225..2497)
                     /locus_tag="PLV68_16860"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRE02972.1"
                     /translation="MRAARRAWMADEELSLAPKEFDLLVLLATHHGQTLRRETIMTEV
                     WDENWWGSTRTLDTHVAALRRKLGDTNDTPERIITIRGIGFRYDSD"
BASE COUNT          374 a          804 c          813 g          363 t
ORIGIN      
        1 gccacccgca tcgcccagac cgtcgccaac cacgcgatcg tgcccgtatc gccccgtggg
       61 ttcgacgtgt tcgcgcagaa ccccacccac gccatcgccg acgtcagcgg gtactacctg
      121 ggcacgcccg caccatcgcc attcggtggc ccgcagaacg tcgaccccac accggtgttc
      181 tgccatggct ttaccagcga ggtcatccag ttggcccnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnaagcggtc gacggatcgt
      301 acggctggac cacccagcag gccgtcatgg cctaccagaa gtactaccgc atgtcggcca
      361 gcggtcgcat cgacgcggcg accgcgacca agctcagcta tcccaactgc aagccgaccg
      421 ccggcaccaa cagcggcacg ctgatggaga tcgacaaggg caagcaactc ggcttcttca
      481 tccgcaacgg ccagttgctg tgggtgatca acgtgtcgac cggcggcaac tatttctacg
      541 aagacgagat caacggcaag aagtacctgg accgggccta caccgacgtc ggcaacttca
      601 gcgtctaccg cgtcagcgac aaggtccgct acgagggcac gctcggcacc atgtaccggc
      661 cccgcttcgt catccgnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnga tgtgaggcac tggccgtcga tgatgtgagg
      841 cactggccgt cgatgatgtg aggcactggc ctcttgcgcc tggcggcagc tttggccgct
      901 gcctcacgtg cggctcgtcg ctggcgcttc cctcgccgcc gtgcgctgcg agccacgtgg
      961 cgcgctcagc ggtctgctac acggcggcgg ggtacgtcac gaccagggtg gtgggggagc
     1021 gttcggtgag ttccaagcgg ccgccatcgg cttgggccag gcggcgggcg agcgagaggc
     1081 cgcggccgtg cggggcagcc gggtcgtccg agccgcggaa cagtcccttg gcggtttcgg
     1141 cgtcgatgcg gccttcgtcg cgcacgcgca actggcggtc ggagacgtgg atcgtgacga
     1201 cgccctgccc gtgagtgagc gcgttgtcga gcaggatgtc gatgatctgg ccgacgaagc
     1261 ccggcgtggc gatcaccggg ctgccactgc ccgagcagta cagcaaccgc ttgcgctgtg
     1321 agtagcgctg caaccagtcg ccgcggtgct cgctggcgat gtcacgcaac tgcaccgtca
     1381 tccgtgcacc accgcgccca ccacgggccg gccccttcgc caacatcgcc cacggcaact
     1441 cgtcgatcgt ggccgtcatg cggtcggtct gcgacagcgc atgggagacg tcgtcgtgca
     1501 cgtcggggtg catcgccgcg agcagctcca gttgcagggc gatgccggtc aagccggtgc
     1561 gtagctggtg ggtggcgtca ccggtgaagc tgcgttcggc gatcagggtc tggtcgagcc
     1621 gcgacgcgct ggccgacagg gtggagcgga tgtcgtcgat ctcgcggatc cccgacggcg
     1681 gtggtagcgc ggcgctgaag tcgcctgccc ccaaccgccc ggccgacgcg gccaactgtt
     1741 cgagcggacg ggtgaggcgg gcaccgaaga tcgcggccag cactgcagcg agcagcacac
     1801 cgcccgcagc gagcacgccg agcagcatga actggcgggt cagccggtcg cggagctcgg
     1861 agtcgtcggt gctgacgtcg accaccctgc cggacaccgc gacgccgtag ccgtgccggg
     1921 cggtgtcggc ctgcggaccg aactggacgt cgactacccc gttgagcgac accaccacac
     1981 gggtgtcgtg gggcaccatc gtgatcaaca gcgcgacgtc gatggcgcgg tgggcgcgca
     2041 cgtcctcgtc gatgacggcg acgatgctgt cggcgtgggc gtcgaggcgc ccctgcagtt
     2101 cggcctcggt ggcgcgcttc aacagcagca ccacgggcgt ggccaacacg gccaacacca
     2161 tcatcgacac gaggatggtg ttgcggacca gccgtctgcg cacaggtgtc ctcggccggg
     2221 ccggtcagtc ggagtcgtag cggaagccga tgccgcggat ggtgatgatc cgctccgggg
     2281 tgtcgttggt gtcacccagc ttgcggcgca acgcggcgac gtgggtgtcg agggtgcggg
     2341 tgctgcccca ccagttctcg tcccacacct cggtcatgat cgtctcgcgc cgcagggtct
     2401 ggccgtggtg ggtggcgagc agcaccagca ggtcgaactc cttcggtgcc agcgacaact
     2461 cctcgtcggc catccacgcc cgccgcgcgg cgcgcaaggg ccacgccttc gcgatcgagc
     2521 gcgaggccta ccgcgccttc gaacggggtt t
//
LOCUS       DAORNF010000506         2546 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_30762_length_2546_cov_2.121638, whole genome shotgun sequence.
ACCESSION   DAORNF010000506 DAORNF010000000
VERSION     DAORNF010000506.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30762_length_2546_cov_2.121638"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            17..>236
                     /locus_tag="PLV68_16865"
     CDS             17..>236
                     /locus_tag="PLV68_16865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02973.1"
                     /translation="MRQFLTWRVWAAFGALVGLTVLLVVLVRADDPLPSRLVRPPIDA
                     PDTPPEHVVDFISIVYQVQPANRFFVLDG"
     assembly_gap    237..276
                     /estimated_length=40
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <277..763
                     /locus_tag="PLV68_16870"
     CDS             <277..763
                     /locus_tag="PLV68_16870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02974.1"
                     /translation="IVEGTPGSISCPDYREVGRCVVVADLLGDAVVWFELVPRQPNEK
                     VTTAPIVEILDDGYVRLANGWVVRHANRVDRRCSQETGSLSEFIATFGLASTTIIDVA
                     TQRVTAVVCADTEAIETTTTRAPATTTSIPAETTVAPPEGEDPANENNAPAGNADNEE
                     G"
     gene            841..1398
                     /locus_tag="PLV68_16875"
     CDS             841..1398
                     /locus_tag="PLV68_16875"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HRE02975.1"
                     /translation="MSALDDRPHSALLVIDMQKGVVTGSHDVEQVAANIATLVDKARA
                     EGVPVVWVQHSDDGMQQGSPEWEYIDELVRAADEPVVHKRYGDSFEDTDLEALLADRG
                     VGRLVVTGAQTDACIRSTLHGGFARGYDVTLVSDAHTTEDMREWGAPMGPGEAIAYTN
                     LYWSFTNAPGRTAATIPTAEITFAD"
     gene            complement(1570..>2546)
                     /gene="nhaA"
                     /locus_tag="PLV68_16880"
     CDS             complement(1570..>2546)
                     /gene="nhaA"
                     /locus_tag="PLV68_16880"
                     /inference="COORDINATES: protein motif:HMM:NF018648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HRE02976.1"
                     /translation="IKREIGVGQLRDRRAVGLPVIAALGGMIVPALIFTAFNAGGPGA
                     DGWGIPMATDIAFALGVVTLLGSRVPPSVKVLLLTLAIADDIGAIVVIALFYSDDLAV
                     GWLVAAVAITALVIVMRRVGVVHPLPFVAAGLALWAAVYESGIHATIAGVVMGLTMPA
                     RPAVDGGESPCDRLIGMLHPWTSFVVVPLFALANAGIGLSVDAISDPSSVLVGVAVAL
                     VVGKLIGVVGFSWLAVRVGIGRLPAAATWRHIVGVGALAGIGFTVSLFITGLAFDAPT
                     LQADAKVGIFGASIVAALAGAAVFASARDRGVVSTVCHIAAGSEDSAR"
BASE COUNT          455 a          889 c          807 g          355 t
ORIGIN      
        1 cctactctgc acggcgatgc ggcagttcct gacgtggcgc gtgtgggccg ccttcggcgc
       61 gctcgtcggt ctgacggtgc tgttggtggt gctggtgcgt gccgacgatc cgctgccgtc
      121 ccgtctcgtg cgccccccga tcgacgcgcc ggacacaccg cccgagcacg tcgtcgactt
      181 catctcgatc gtgtaccagg tgcagccggc gaatcgcttc ttcgtgctcg acggtgnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncatc gtcgagggca cgccgggttc
      301 gatcagctgc cccgactatc gcgaggtcgg ccgctgtgtc gtggtcgcgg acctgctcgg
      361 tgatgccgtg gtgtggttcg aactcgtgcc tcgccaaccg aacgagaagg tgaccaccgc
      421 gccgattgtc gagatcctcg acgacggcta tgtgcgtctg gccaacgggt gggtggtgcg
      481 ccacgccaac cgggtcgatc ggcgttgcag ccaggagacc gggtcgctca gcgagttcat
      541 cgccaccttc ggcctggcca gcaccacgat catcgacgtc gccacccagc gcgtcaccgc
      601 cgtggtctgc gccgacaccg aagccatcga aaccaccacg acccgcgccc cagcgacgac
      661 cacctccatc cccgcagaga ccacggtcgc cccgcccgag ggtgaagacc ccgccaacga
      721 gaacaacgcc ccggccggca atgccgacaa cgaagaaggc tgacagacgg tcgtcgtatc
      781 gcacgatcaa ccgagcagct tgtgcgaacg tgtgatcggg tcggtgctac ggtggtcggc
      841 atgagtgctc ttgacgatcg tccccattcg gcgctcctcg tgatcgacat gcagaaaggt
      901 gtcgtcaccg gtagccacga cgtcgaacag gtggcggcga atatcgccac gttggtcgac
      961 aaggcgcgcg ccgaaggtgt accagtggtg tgggtgcagc actccgacga cgggatgcag
     1021 cagggttcgc ccgagtggga gtacatcgac gaactggtcc gtgccgccga cgagccggtg
     1081 gtgcacaagc gctatggcga ctcgttcgaa gacaccgacc tcgaagcgct gttggccgac
     1141 cgaggcgtgg gccgcctggt ggtgaccggt gctcagaccg atgcctgcat ccgctccacg
     1201 ctgcacggtg gcttcgctcg tggttacgac gtcaccctgg tgagcgacgc ccacaccacc
     1261 gaggacatgc gcgagtgggg tgcgccgatg gggccgggcg aagcgatcgc ctacaccaac
     1321 ctgtactggt ccttcaccaa cgctcccggc cgcacggccg ccaccatccc cacggcagag
     1381 atcaccttcg ccgactgacg gcgactgata gggcgacggt cccgtcgccg gtcacgcctg
     1441 gtacgaccag acctgctcgt cgagattctg caacagcgcg tgcagcgcgc cgtacaccgt
     1501 tgtcgtgtcg gcgtacgccg tcatgtatgc ggtccccgcc accccaggaa tgagtcggtc
     1561 acatgccatc taccgggcgg aatcttcgct acccgcagcg atgtgacaga ccgtggagac
     1621 cacaccgcgg tcgcgcgcag acgcgaagac ggccgctccg gcgagcgcgg cgacgatcga
     1681 cgcgccgaag atgccgacct tggcatcggc ctgcagtgtc ggcgcgtcga acgccagccc
     1741 ggtgatgaac agcgacaccg tgaagccgat gcccgccagc gcacccacgc cgacgatgtg
     1801 ccgccacgtc gctgcagcgg gcagccggcc gatgccgacc cgcacggcca gccacgagaa
     1861 cccgacgacg ccgatcagct tgcccaccac caatgcgacg gcgacgccga ccagcaccga
     1921 cgacgggtcg ctgatcgcat cgaccgacag tccgatcccg gcgttggcca acgcgaacag
     1981 cggcacgacc acaaagctgg tccacggatg caacatcccg atcagccgat cgcacggcga
     2041 ctcgccgccg tccacggccg gacgggcagg catcgtcagg cccatgacga caccggcgat
     2101 cgtggcgtgt atccccgact catagaccgc cgcccacagc gccagcccgg cggcgacgaa
     2161 cggcaacggg tggacgacgc cgacgcggcg catcacgatc accaacgccg tgatcgcaac
     2221 ggcggccacg agccaaccga ccgcgaggtc gtcgctgtag aacagggcga tcaccacgat
     2281 cgcgccgatg tcgtccgcga tcgccaacgt caacagcagt accttgaccg agggcggcac
     2341 ccgcgagccg agcaacgtga cgacaccgag cgcgaacgcg atgtcggtcg ccatcgggat
     2401 gccccagccg tcagcgccgg gaccacccgc gttgaacgcg gtgaagatca gggcgggcac
     2461 gatcatgccg ccaagggcgg cgatcaccgg caacccgacc gcgcggcgat cgcgtaactg
     2521 gccgacgccg atctcgcgct tgattt
//
LOCUS       DAORNF010000507         2544 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_30798_length_2544_cov_1.472881, whole genome shotgun sequence.
ACCESSION   DAORNF010000507 DAORNF010000000
VERSION     DAORNF010000507.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30798_length_2544_cov_1.472881"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>342
                     /locus_tag="PLV68_16885"
     CDS             <1..>342
                     /locus_tag="PLV68_16885"
                     /inference="COORDINATES: protein motif:HMM:NF016129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HtaA domain-containing protein"
                     /protein_id="HRE02977.1"
                     /translation="ASSPVAAAPEAVGGGWLHWGVKSAFSSYITGPIANGSITLTPPA
                     TRDDVGTFAFVASSGTFDRAASTVTAGFDGGIWFRGHDYENGPLLEVAFTNVRVEVSG
                     NSGVVIADVASR"
     assembly_gap    343..530
                     /estimated_length=188
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <531..>888
                     /locus_tag="PLV68_16890"
     CDS             <531..>888
                     /locus_tag="PLV68_16890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02978.1"
                     /translation="KSLLSLAVMFVLSTTTVQHVPTFASFNPARLLDTRADGETTDGL
                     FIGHGRIDPGVEFALQVGGRGGVEADDAAVSLNVTATDATGTGFVTVWPCGTAKPLAS
                     SLNFVRSEAVANAVIT"
     assembly_gap    889..994
                     /estimated_length=106
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1005..>1315
                     /locus_tag="PLV68_16895"
     CDS             1005..>1315
                     /locus_tag="PLV68_16895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02979.1"
                     /translation="MLDTRGPADGAATVDGQFLNDGIKTAGSMTQLTVAGRGNVPLNA
                     AAVVLNVTVTQPTGGGFVTVWNCGVAPPNASTLNFVTGQTAPNLVVVKVETNQPGLVG
                     LG"
     assembly_gap    1316..1325
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1326..1767
                     /locus_tag="PLV68_16900"
     CDS             <1326..1767
                     /locus_tag="PLV68_16900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02980.1"
                     /translation="GANAHLIADVTGYFPPGSGFSPLTPARLADTRPPAEDGQTTDGA
                     FLGNGRIGSTQTYPFSVANRGGLPAAPTAVALNVTVVAPTGSGFVTVWPCGDNRPWAS
                     SVNFAAGDVAPNAVITKVGTNGTVCVFASGAATHVVVDVTGYFD"
     gene            complement(1804..2424)
                     /locus_tag="PLV68_16905"
     CDS             complement(1804..2424)
                     /locus_tag="PLV68_16905"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRE02981.1"
                     /translation="MIGGSIAAHREAITSRIFDALGELMHTEGYEAISLADVAAKADL
                     ARTTMYNYFPDKDALAVAYAEREVDKYVASLTDQLARADNPVDQLRLYIRRQLRYFAT
                     HHMPPGRTLQSMLPTTTYQRLVHHVQLLETILAGILGDGEATGYFTPNTAAALPLVAA
                     CVNRGGPGAFDDVDLDDEIDATETFVLRALGARLDAEGKAVVVQSK"
BASE COUNT          339 a          771 c          761 g          369 t
ORIGIN      
        1 cgcgtcgtct cccgttgccg ccgcgccgga ggcggttggc ggcggctggc tgcactgggg
       61 cgtcaagtcg gcgttttcca gctacatcac cggtccgatc gcgaacgggt cgatcacgct
      121 caccccaccc gccacccgcg acgacgtcgg caccttcgcc ttcgtcgcca gttcgggaac
      181 gttcgatcgc gccgcatcga ccgtgacggc gggattcgat ggtggcatct ggttccgtgg
      241 tcacgactac gagaacggac ccctgctcga agtggccttc accaacgtgc gggtcgaggt
      301 cagcggaaac agcggtgtgg tgatcgccga cgtcgcgtcg cgnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn aaaaatcact
      541 tctttccctg gccgtgatgt tcgtgctgag caccaccacg gtgcagcatg tgccgacgtt
      601 tgcttcgttc aacccggctc gcctgctgga cacccgcgcg gacggtgaga ccaccgacgg
      661 gttgttcatc ggccacggcc ggatcgaccc cggcgtcgag ttcgccctcc aagttggagg
      721 ccgcggcggc gtcgaggccg acgacgcggc ggtgtcgctg aacgtcaccg ccaccgatgc
      781 gaccggcacc gggttcgtca cggtctggcc gtgcggtacc gccaagccgc tggcctcgtc
      841 actgaacttc gtgcgcagcg aggcggtggc caacgcggtc atcaccaann nnnnnnnnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnccctgc ccgcttgctc gacacgcgcg
     1021 gccccgccga cggtgcggcc acggtcgacg gtcagttcct caacgacggc atcaagaccg
     1081 cggggagcat gacccaactc accgtggccg gccgtggcaa cgtcccgctg aacgcggccg
     1141 cggtggtgct caacgtcacc gtcacccaac cgaccggcgg cggcttcgtc accgtttgga
     1201 actgcggtgt tgccccaccg aatgcgtcga cgctcaactt cgtgacgggg cagaccgctc
     1261 ccaacctggt ggtggtcaag gtcgagacca atcagccggg gttggtgggc ctgggnnnnn
     1321 nnnnncggcg ccaacgcgca cctcattgcc gacgtcaccg gctacttccc acccggcagc
     1381 gggttcagcc cgctgacccc ggctcgcctg gccgacaccc gcccgccggc cgaggacggc
     1441 cagaccaccg atggcgcgtt cttgggcaac ggtcggatcg gatcgacaca gacgtacccg
     1501 ttcagcgtcg ccaaccgcgg tggtctgcct gccgcgccca cagcggtcgc gctcaacgtg
     1561 acggtcgtcg ctccgaccgg atcgggtttc gtcacggtgt ggccgtgcgg tgacaaccgc
     1621 ccgtgggcct cgtcggtcaa cttcgcagcg ggcgacgtcg cgcccaacgc ggtgatcacc
     1681 aaggtcggta ccaacggcac ggtgtgcgtg ttcgcgagcg gggcggcgac ccacgtggtg
     1741 gtcgacgtga ccggctattt cgactgaccg gctacgtcga ctgccggtta cgtcgactgc
     1801 ctactatttc gactgaacga cgactgcctt gccctcggcg tcgagacggg cgccgagggc
     1861 acgcagcacg aacgtctccg tcgcgtcgat ctcgtcgtcc aggtcgacgt cgtcgaacgc
     1921 gcccggccca ccccggttga cgcaggccgc gaccaggggc agtgcagcgg ccgtgttggg
     1981 cgtgaaatac ccggtggcct cgccgtcgcc gaggatgccg gccaggatcg tctccagcag
     2041 ttgcacgtga tggaccaggc gctggtacgt ggtggtgggc agcatgctct gcagggtgcg
     2101 ccccggcggc atgtggtgcg tcgcgaagta gcggagctgg cggcggatgt acaggcgaag
     2161 ctggtcgacc gggttgtcgg cgcgggcgag ctgatcggtc agcgaggcga cgtacttgtc
     2221 gacctcgcgc tcggcatacg ccaccgccaa ggcgtccttg tcggggaagt agttgtacat
     2281 ggtggtgcgg gccaggtcgg ccttggccgc cacgtcggcg agcgagatcg cctcgtaacc
     2341 ctccgtgtgc atcaactcgc cgagggcgtc gaagatgcgg ctggtgatcg cttcgcgatg
     2401 ggccgcgatc gagcccccga tcacccgtgg cacagcatgg ccctggacga ttggcgcgcg
     2461 atcacgggcc atatcgtggc acgcgcgagg ccgatctcca catctgaccg gcaacctgac
     2521 cggcgacctg accggcgacc tgac
//
LOCUS       DAORNF010000508         2542 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_30828_length_2542_cov_1.467230, whole genome shotgun sequence.
ACCESSION   DAORNF010000508 DAORNF010000000
VERSION     DAORNF010000508.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30828_length_2542_cov_1.467230"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="PLV68_16910"
     CDS             complement(<1..289)
                     /locus_tag="PLV68_16910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02982.1"
                     /translation="MNTVATPSPDVDPLLAVTPLLDVVGANLHVPIVPDGERRFVHLD
                     YAASAPSLVSVADEIRRFLPWYSSVHRGAGFTSTISSERLDAARDTIRRFVG"
     assembly_gap    307..333
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<334..>989)
                     /locus_tag="PLV68_16915"
     CDS             complement(<334..>989)
                     /locus_tag="PLV68_16915"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02983.1"
                     /translation="FAHLLGSPRFALQLDLLQQPGATAPPYAEQIDDVLALLAGTYTD
                     AAGTEAHAVPGEGAALDVWILGSSGGDSATVAGERGLPFAANYHVSPATVIEAVTAAF
                     RPRPGRDRPWVAVSADVVVGENAAHARELATGYAAWVHSIRSGAGAIPFPTPDEARAM
                     PWSDADQALVQDRLDTQFVGSPADVADQLAVLAAETGADELVVTTITHDHADRVRSYE
                     "
     assembly_gap    990..1097
                     /estimated_length=108
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1098..1285)
                     /locus_tag="PLV68_16920"
     CDS             complement(<1098..1285)
                     /locus_tag="PLV68_16920"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HRE02984.1"
                     /translation="MIGLIANATSTLRVGSGAVLMGHQTPLSIVEQFGILDAVHPGRI
                     DLGLGRSGSRLFASERIA"
     assembly_gap    1306..1487
                     /estimated_length=182
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1562..>1902
                     /locus_tag="PLV68_16925"
     CDS             1562..>1902
                     /locus_tag="PLV68_16925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02985.1"
                     /translation="MSTDAIDVIDAPADAGVVPSGRPATVPVGAPVASLRIVSRKRPA
                     RWIATAVIAVLVAMLVNGLVTNPKWDWPVFFEYFTADSILKALRTTLWLTLWGTVVGF
                     GQYSQSIFDYHP"
     assembly_gap    1903..1912
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1913..>2289
                     /locus_tag="PLV68_16930"
     CDS             <1913..>2289
                     /locus_tag="PLV68_16930"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRE02986.1"
                     /translation="GTVLAAMRLSKSPLLQAVSWTYTWLFRSVPLIVNLLFWYNLAYL
                     YRELSIGIPFGPSFWRASTLDLFSPMAAAITGLAVHQAAYSAEVMRSGLLSVDHGQRE
                     AAAALGIPRWRQFRRIVLPQEMRT"
     assembly_gap    2290..2299
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          346 a          694 c          775 g          390 t
ORIGIN      
        1 cgccgacgaa ccgccggatc gtgtcgcgcg ccgcgtccaa gcgctcgctg ctgattgtcg
       61 acgtgaagcc cgcaccgcgg tggacgctgc tgtaccacgg caggaatcgg cggatctcgt
      121 ctgcgacaga gacgaggctt ggcgcgcttg cggcgtagtc gaggtggacg aatcggcgct
      181 cgccatcagg aacgatcggg acgtgcaggt tggcaccgac cacatcaagc aatggggtga
      241 cggcgagcaa cgggtcgaca tcgggagacg gcgtggcgac cgtgttcatg acgcggtcac
      301 cagcgcnnnn nnnnnnnnnn nnnnnnnnnn nnncctcgta cgagcggacg cgatcggcgt
      361 ggtcgtgggt gatcgtcgtg acgacgagtt cgtccgcgcc ggtctccgcg gcgagcacgg
      421 cgagttggtc ggcgacgtcg gctggcgaac cgacgaactg cgtgtcgagg cgatcttgca
      481 cgagtgcctg gtcggcgtcg ctccacggca tggcgcgcgc ctcgtccggt gtggggaacg
      541 ggatcgcgcc cgcaccgctg cggatgctgt gcacccacgc cgcgtagccc gtcgccaact
      601 cgcgcgcgtg cgcggcgttc tcgccgacca cgacgtcggc cgacacggcg acccacggac
      661 ggtctcggcc cggccgagga cggaacgcgg cggtgacggc ctcgatcacc gtcgccgggc
      721 tgacgtgata gttggccgcg aagggcaggc cgcgctcacc cgcgaccgtg gcgctgtcgc
      781 caccgctgct gccgaggatc cagacgtcga gtgcagcacc ctcaccgggc acggcgtggg
      841 cctcggtgcc agccgcgtcg gtgtaggtgc cggcgagcag tgcgagcacg tcgtcgatct
      901 gttcggcata cgggggtgct gtggcgccgg gctgctgcaa caggtcgagt tgcagcgcga
      961 agcgtggcga gccgagcaga tgcgcgaagn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnttc gcgatgcgtt cagaggcgaa caggcgtgaa ccgctgcgac
     1141 cgaggccgag atcgatgcgg ccggggtgca cggcatcgag gatgccgaac tgctcgacga
     1201 tggagagcgg cgtctggtgt cccatgagca ctgcgccgga gccgacgcgg agggtcgacg
     1261 tggcgttggc gatcaacccg atcatcagcg cgggcgtcgt actcgnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnata aggtgtgacg
     1501 atgtccacat tgtgataaat ccgactagac agatcggaat tatcgagata tggtcctcgc
     1561 catgtccacc gatgccatcg acgtcatcga cgcccccgcc gatgcaggcg ttgtcccctc
     1621 cggtcggcct gcaaccgttc cggtcggggc tccggtcgcg tcgctacgaa tcgtcagccg
     1681 caagcggccg gcgcgctgga tcgcaaccgc cgtcatcgcc gtgctggtcg cgatgttggt
     1741 caacgggttg gtgaccaacc cgaaatggga ctggccggtg ttcttcgagt acttcaccgc
     1801 cgactcgatc ctcaaggcgc tgcgtaccac gctgtggctg acgctgtggg gcaccgtcgt
     1861 cggctttggg cagtacagcc agagcatctt tgattatcac ccnnnnnnnn nnggcaccgt
     1921 gctcgccgcg atgcggctgt cgaagagtcc gctgctgcag gcggtgagct ggacctacac
     1981 ctggctgttc cggtcggtgc cgctgatcgt caacctgctg ttctggtaca acctcgccta
     2041 cctgtaccgg gaactgtcga tcggcatccc cttcggcccg tcgttctggc gggccagcac
     2101 gctcgatctg ttcagcccga tggccgcggc gatcacgggg ctcgccgtgc accaagccgc
     2161 ctactcggcc gaagtgatgc gctctggact cttgtcggtc gaccacgggc agcgtgaagc
     2221 ggccgcggcg ctcggcattc cgcggtggcg ccagttccgc aggatcgtgc tgcctcagga
     2281 gatgcgcacn nnnnnnnnnt tgatcgtttc gtcttgcatc gcgaacagtt ccggagcgaa
     2341 cgaggtcatc agcctgttca agagcacgtc ggtcgtgtac gtgatggcga tcggggaact
     2401 gttctaccaa gtgcgggtca tctacaaccg caacggtgcg gtgtgggcgc tgctgatggt
     2461 ggcaacggtg tggtacctcg tcctgacgac ggtgttgtcg atcgtgcagt actacgtgga
     2521 gcgctacttc gcgcgaggtt cg
//
LOCUS       DAORNF010000509         2539 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_30878_length_2539_cov_2.036232, whole genome shotgun sequence.
ACCESSION   DAORNF010000509 DAORNF010000000
VERSION     DAORNF010000509.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2539
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30878_length_2539_cov_2.036232"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..781
                     /locus_tag="PLV68_16935"
     CDS             <1..781
                     /locus_tag="PLV68_16935"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HRE02987.1"
                     /translation="MTEEGADYAAALSEAQRLGFAERDPTADVEGFDAGAKAAIIATI
                     AFGNKVVAGDVYHEGISGISASDIAMATRLGYVVKLLAICEQDPGSGEIAVRVHPAMV
                     PAQHPLASVRESYNAVFVEGAAVGSLMFYGRGAGGLPTASAVLGDVIDAAVNLAQGTH
                     GSLGSFGRAVIRPIDETSSEYLLSLDVADRPGVLHQVTGVFARNDVSIRAAEQEGLRD
                     DARLVFITHSARESSVQACLRELRDLDVVKRVGGLLRVVGS"
     gene            778..2169
                     /gene="thrC"
                     /locus_tag="PLV68_16940"
     CDS             778..2169
                     /gene="thrC"
                     /locus_tag="PLV68_16940"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HRE02988.1"
                     /translation="MRYVSTRGTAPELGFCDALLAGLASDGGLYVPTEWPELPDLSPA
                     RTYADAAAAVMAPFVGDEIAPATFHALCAEAYASFRHDAVVPLVQVGANEWLLEQFHG
                     PTLAFKDVALQLVGRLFDHVLGQRGERVTIVGATSGDTGSAAIDGVKGCAHVDIVILY
                     PAGRTSDVQRRQMTTVDAPNVHTVAIEGTFDDCQDLVKAMFNDAPFRQRLQLSAVNSI
                     NWARVMAQIVYYVTASRSLGATAERPVTFSVPTGNFGNVLSGWIARRMGASIQDFVVS
                     SNSNDILTRFITANDMTARAVVPTLSPSMDIQVSSNFERLLFEMNGRDGGMTADQMQR
                     FRHTGQLQVEADQRDEWITPSFRAAQCDDTSTLAEIRRVYQASGQLIDPHTATGTAAV
                     RQLGVADEGRPVITLATAHPAKFPDAVQQATGVHPSLPPHLADLFDRVERVNVAPNDL
                     GAVQRFVEQAVQH"
     assembly_gap    2277..2310
                     /estimated_length=34
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          395 a          852 c          855 g          403 t
ORIGIN      
        1 gatgaccgaa gagggtgccg actacgccgc ggcgctgtcc gaagcgcagc ggttgggctt
       61 tgccgaacgc gaccccaccg ccgacgtcga aggcttcgac gcgggggcca aggcggccat
      121 catcgccacg atcgcgttcg gtaacaaggt cgtcgcgggc gacgtctacc acgagggcat
      181 cagcggcatc agcgcgtccg acatcgcgat ggcgacgcgg ctgggctacg tggtcaagct
      241 gctcgccatc tgcgaacagg accctggcag cggcgagatc gccgtgcgcg tgcacccggc
      301 gatggtgccc gcgcagcatc cgttggccag cgtgcgcgag agctacaacg cggtgttcgt
      361 cgagggtgcg gcggtcggct cgttgatgtt ctacggccgt ggcgcaggag gcctgcccac
      421 cgccagcgcg gtgttgggtg acgtgatcga cgccgcggtc aacctggctc agggcactca
      481 cggttcgctc gggtcgttcg gtcgggcggt catccgcccc atcgacgaaa ccagctccga
      541 gtacctgctc agcctcgacg tggccgaccg tccgggcgtg ttgcatcagg tcacgggcgt
      601 gttcgcccgc aacgatgtca gcatccgcgc cgccgagcag gaaggcctgc gcgacgacgc
      661 ccggctggtg ttcatcaccc actcggcccg cgaatcgtcg gtgcaggcat gtctgcgcga
      721 gctgcgcgac ctcgacgtgg tcaagcgggt cggcggtctg ttgcgggtgg tggggtcgtg
      781 aggtacgtct ctacccgtgg taccgcaccc gagctcggct tctgcgacgc actgctggcc
      841 ggattggcct ccgacggtgg cctgtacgtg cccaccgaat ggcccgaatt gccggacttg
      901 tcgccagcgc gcacctacgc ggatgcggct gcggcggtga tggcgccgtt cgtcggcgac
      961 gagatcgccc cggccacgtt ccacgcgctg tgtgccgagg cctatgccag cttccgtcac
     1021 gacgcggtcg ttccgttggt gcaggtcggc gccaacgagt ggctgctcga gcaattccac
     1081 ggcccgacgc tggcgttcaa ggacgtcgcc ctgcaactgg tcgggcggct gttcgaccac
     1141 gtgctcggcc agcgcggtga gcgggtcacc atcgtcggcg ccaccagcgg cgacaccggc
     1201 agcgcggcga tcgacggggt caaggggtgc gctcacgtcg acatcgtgat cctctacccg
     1261 gcggggcgca ccagcgacgt gcagcggcgc cagatgacca cggtcgacgc gccgaacgtg
     1321 cacaccgtgg cgatcgaggg caccttcgac gactgccagg acttggtcaa ggcgatgttc
     1381 aacgacgcgc ccttccgcca acggctgcag ctctccgcgg tcaacagcat caactgggcc
     1441 agggtgatgg cccagatcgt ctactacgtc accgcgtccc gctcgctggg cgccacggcc
     1501 gagcggccgg tcaccttcag cgtgcccacc gggaacttcg gcaacgtgct cagtggctgg
     1561 atcgcccgcc ggatgggcgc gtcgatccag gatttcgtgg tgtcgtccaa cagcaacgac
     1621 atcctcaccc gcttcatcac cgccaacgac atgaccgccc gtgcggtggt accgactctc
     1681 agcccgtcga tggacatcca ggtgtcgtcc aacttcgagc ggctgctgtt cgagatgaac
     1741 ggccgcgacg ggggcatgac cgccgatcag atgcagcgct tccggcacac cggtcagctc
     1801 caggtcgaag ccgatcagcg cgacgagtgg atcacgccca gctttcgggc tgcgcagtgc
     1861 gacgacacct ccacgctggc cgagatccgc cgcgtgtatc aggccagcgg ccagctgatc
     1921 gacccgcaca ccgcaaccgg aaccgccgcg gtccgtcaac tgggcgtggc cgacgagggt
     1981 cgcccggtga tcacgttggc cacggcacac ccggccaagt ttcccgacgc ggtgcagcag
     2041 gccaccggcg tgcacccgtc gttgcctccc cacctggccg atctgttcga ccgggtcgag
     2101 cgggtcaacg tggcccccaa cgatctgggt gcggtgcagc ggttcgtcga acaggccgtg
     2161 cagcactgac acggtcgcgg tctggcgacg gttggcgagg tgtctgatcg cggcgcggtt
     2221 gcccgcgatt ggttgcacgg cgcgccgtgg cgtcgatagg gtgcgagcgc ccgtgcnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gtggaaccga ggctgtgttc gagaatctcg
     2341 ctggtcgtgg gaaccagcgg tgtggcgtca tggcgtgcac cgcaccgccc cacgtctgaa
     2401 ccccgccaac ggtcgatacg gcgaccgacc cctgaaagta ggaatcgtgg cagccaatgc
     2461 cctcgaacgt tctgtgctcg agtcgaaaga ccgcgaacaa ctggtagcca tcgccaatgc
     2521 actcggcgtc aagtcgacg
//
LOCUS       DAORNF010000510         2538 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_30894_length_2538_cov_1.958921, whole genome shotgun sequence.
ACCESSION   DAORNF010000510 DAORNF010000000
VERSION     DAORNF010000510.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2538
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30894_length_2538_cov_1.958921"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..681)
                     /locus_tag="PLV68_16945"
     CDS             complement(<1..681)
                     /locus_tag="PLV68_16945"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRE02989.1"
                     /translation="MDFHLDDDQTAILLSRHAGAARVRALGGDAPAYDHDLEHQLDQA
                     GFLDVVTPGAGDRLNAALVQEAISLHLGCVSTSARLLVAPSLPLGLEGPVAVVRADHR
                     GPARFLADACSVVLVANDGVRLATPARGDVSRPVSRLGWPVGMAADVAAGVALPEVDP
                     REITTWSRIALAIELVGAMRFATDLTVGYVIDREQFGRSIGSFQALQHRAVDHYIRIA
                     LSRSALDRA"
     gene            complement(681..1826)
                     /locus_tag="PLV68_16950"
     CDS             complement(681..1826)
                     /locus_tag="PLV68_16950"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRE02990.1"
                     /translation="MEFGWDDEHSSYRTELVSFLNDELPDHWHGGTAVLGSSDNTEYS
                     RRFAALLAERGWLTPHWPREFGGHDASPWMLAVLGEELWSRGEPRGPQYMNTNWIGPS
                     IMAHGTPEQQERYLPAIAAGNAIWCQGFSEPDSGTDLASLRLRADRDGDDYVLNGTKI
                     WTSYADVAEHCYLLVRTDPTAAGPRGISVLLVDLPVSGLTIRTIPGVVGEHSFNELVF
                     TDARVPVANRLGDENKGWEVVRQALSYERVGAPRWARAAFVLDEAIAAARTAGRTFDA
                     MAVEAIGVAKTACEAARILYYRVIAERALKLPQSANANIARVAMVKSERLVAEACLAI
                     DRCDVLVYGSRSDHQLRKSMAAGIAAGTYEVQLNLIARLHLGLPKGS"
     gene            complement(1915..>2538)
                     /locus_tag="PLV68_16955"
     CDS             complement(1915..>2538)
                     /locus_tag="PLV68_16955"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRE02991.1"
                     /translation="VALAQRARTGKGSVVESPMVGAALATTAEQVIDFAVSGRLQTRL
                     GNASPLIDQDVFRCAGEDAWLAVSFPHGSALESLRAVTGTATFEALARWCADRSATAA
                     ADALTAAGIPAAEVVWAHETAANPQLGARSFFEIVEHPVCGTHPYASWPVRFGSGPHR
                     WNRVAAPTMGQHNGVILSELGYSAADIAELERERIITSRVIIDQHGW"
BASE COUNT          438 a          875 c          832 g          393 t
ORIGIN      
        1 cgcgcgatcc agcgccgagc gcgacagcgc gatccggatg tagtggtcga ccgcgcgatg
       61 ctgcaacgcc tggaacgagc cgatcgagcg cccgaactgt tcccggtcga tgacgtaacc
      121 aacggtgagg tcggtcgcga agcgcatcgc gccgacaagc tcgatcgcaa gtgcgatcct
      181 gctccacgtg gtgatctcac gtgggtcgac ctccggcaga gcaacacccg cagcgacgtc
      241 agcggccatg ccgaccggcc accccaatcg cgagaccggc ctcgacacgt cgccccgcgc
      301 aggcgtggcc aaccgcacac cgtcgttggc cacgagcacc acagagcagg cgtcggcgag
      361 aaaccgtgcg ggcccccggt gatctgcacg caccaccgcc accggaccct cgagcccaag
      421 gggaagcgag ggtgcgacga ggagacgggc actcgtcgac acacaaccaa ggtgcaacga
      481 gatggcctcc tgtaccagcg ccgcgttgag gcggtcaccg gcgcccggtg tgaccacgtc
      541 gagaaatcca gcctggtcca gttggtgctc gaggtcgtgg tcgtacgccg gggcatcgcc
      601 tccgagcgcc cgcacccgcg ccgcacccgc gtgtcgcgac agcaggatcg cggtctggtc
      661 gtcgtcgagg tggaaatcca tcacgatccc ttcggcagcc cgaggtgcag tcgggcgatc
      721 agattgagtt ggacttcgta cgtccccgcc gcgatgccgg cagccatcga cttgcgcagt
      781 tggtggtccg accgtgaccc gtagacgagc acgtcgcacc ggtcgatcgc caggcacgcc
      841 tcggcgacga ggcgctccga cttcaccatc gcgacgcggg caatgttcgc gttggcggac
      901 tgcggcagct tgagcgcacg ctcggcgatc acgcggtagt agaggattcg tgcagcctcg
      961 cacgccgtct tggcgacacc gatcgcctcg accgccatgg cgtcgaacgt gcgacccgca
     1021 gtgcgggctg cggcgatcgc ttcgtcgagc acgaacgcgg cgcgcgccca acgcggcgcc
     1081 ccgactcgct cgtaggagag cgcctggcgg acgacctccc aacccttgtt ctcgtcgccc
     1141 aggcggttcg ccacgggcac acgcgcatcg gtgaacacca actcgttgaa cgaatgctcg
     1201 cccacgaccc cagggatcgt ccggatcgtc aggccggaga cgggaagatc gacgagcagc
     1261 accgagatgc cacgcggtcc ggccgccgtc gggtcggtgc gcacgagcag gtagcagtgc
     1321 tcggctacat cggcgtacga cgtccagatc ttggtgccat tgagcacgta gtcgtcacca
     1381 tcgcggtcgg cgcgcaggcg caaggaggcc aggtcggtgc ccgagtcggg ttcggagaag
     1441 ccttggcacc agatcgcgtt gccggcggcg atcgcgggaa gataccgctc ctgctgctcg
     1501 ggggtgccgt gggccatgat cgacggaccg atccagttgg tgttcatgta ctgcgggcca
     1561 cggggctctc cgcgcgacca cagctcttca cccagcaccg caagcatcca cggcgacgcg
     1621 tcgtgtccgc cgaattcgcg gggccaatgc ggcgtcaacc aaccccgttc ggcgagcagt
     1681 gcggcaaagc gccgcgagta ctcggtgttg tcggacgatc cgagaaccgc cgtgccgccg
     1741 tgccagtggt caggcagttc atcgttgaga aacgacacga gttcggttcg gtaggagctg
     1801 tgctcgtcat cccagccgaa ctccatatgt accttcttcc gtcagtagtg cctgtcaccg
     1861 tggtgatagt tgagtgtact cattattgct cgccgtgcca tctccggctg tcgctcacca
     1921 gccgtgttga tcgatgatca ctcggctcgt gatgatgcgc tcgcgctcga gttcggcgat
     1981 gtccgcagcg ctgtacccga gctcgctcag gatcacgccg ttgtgttgcc ccatcgtcgg
     2041 cgcagcgacg cggttccacc ggtgcggacc ggagccgaac cgaacgggcc acgacgcata
     2101 cgggtgcgtg ccgcacaccg gatgctcgac gatctcgaag aacgatcggg ctccgagttg
     2161 gggattggca gcagtctcgt gcgcccacac cacctcggct gcggggatcc cggcagcggt
     2221 cagcgcgtct gccgcagcgg tggcgctgcg atcggcacac caccgcgcga gtgcctcgaa
     2281 cgtggcggta ccggtgaccg cacgcaacga ctccaacgcg gacccatgtg ggaacgagac
     2341 cgcgagccat gcatcctcac cggcgcacct gaacacgtcc tggtcgatca gcggcgaggc
     2401 gttccccagc cgtgtttgca gccgaccgga cacggcgaaa tcgatcacct gctcggcggt
     2461 ggtcgcgaga gccgccccaa ccatcggcga ctcgaccacg ctgcccttcc ctgtgcgggc
     2521 gcgctgcgcc aatgccac
//
LOCUS       DAORNF010000511         2532 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_30988_length_2532_cov_1.742430, whole genome shotgun sequence.
ACCESSION   DAORNF010000511 DAORNF010000000
VERSION     DAORNF010000511.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2532
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30988_length_2532_cov_1.742430"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            17..>518
                     /locus_tag="PLV68_16960"
     CDS             17..>518
                     /locus_tag="PLV68_16960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02992.1"
                     /translation="MCGIDRKFGQKTRPQGRDVRQSQGPGYTGRQFLRRQPRRRVGDR
                     RLADGADVTHLALDALIGPAVDRFRHRQQFVATACYLIRGLFCVAMSLTLYQLSFYGF
                     ALGLLIAGKASGIVKQALIPSLVRDPSRLVATNARLGRTASIVGAIGASAGLALQQSV
                     LDTRGLL"
     assembly_gap    519..528
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <529..1509
                     /locus_tag="PLV68_16965"
     CDS             <529..1509
                     /locus_tag="PLV68_16965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRE02993.1"
                     /translation="YYNEVEPNIKKGAALAFAAAAIVITRMGLRGETEQDRLPTSLEY
                     AETHAPPVVVGSIGFMAIRGAVGFFVFMLAFTLRQESEPKWVYGVAIFVYGAGAFVGN
                     LVAPVLRKRFSDQQLIAIAIALPTLPTLFGMLGVSRALLLVIATAIGVSTTLGRHGFD
                     SLLQDRASAALRGRAAARYETRFQLVWAIGALIATPISLPPEASMAVLAALYIPALSV
                     FLRASTAAKAFEADVSLDVVGRAGARLATAEQSLSTESYAVAVIDAAAAIDLARLSDA
                     DLSRLQECVDLNRLRLAALEHRETVTHAEAQEAVALARLLLTSIRSPSPE"
     gene            complement(1484..>1854)
                     /locus_tag="PLV68_16970"
     CDS             complement(1484..>1854)
                     /locus_tag="PLV68_16970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE02994.1"
                     /translation="GSKIGPDDTIVTKARVVYVAPTSGSPIPAGSQVAVVGNLADMGD
                     NIELRFADVRDDAIDLDAEGEPVRDDGALLVVGKVPEKRPPVDVSVEVYFNSSTQHDY
                     TLRISRRDGTYVVTQATENE"
     assembly_gap    1855..1906
                     /estimated_length=52
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2221..2308
                     /estimated_length=88
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2309..>2532)
                     /locus_tag="PLV68_16975"
     CDS             complement(<2309..>2532)
                     /locus_tag="PLV68_16975"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HRE02995.1"
                     /translation="AGWSAGGNLAAVVCQRARDEGGPQIAAQLLLAPVTDGTVVRPSM
                     LANAEGYMLTKATMDWFWDHYADPADRTSP"
BASE COUNT          342 a          828 c          819 g          393 t
ORIGIN      
        1 ccacgccgtc ggctgcttgt gcggaataga caggaaattc ggccagaaga cccggccgca
       61 aggccgcgat gtcaggcaga gccagggtcc gggctacaca ggccgccagt tcctgcgccg
      121 acagccccgt cgccgggtcg gcgaccggag actggccgac ggcgcggatg tgacccacct
      181 ggcgctggac gcgctgatcg gcccggcggt cgaccgcttc cgccaccgcc agcagttcgt
      241 cgccaccgcg tgctacctga tccgcgggtt gttctgcgtc gcgatgtcgc tgaccctgta
      301 ccagctctcg ttctacgggt tcgcgctcgg cctgttgatc gcgggcaagg cgtcgggcat
      361 cgtcaagcag gcgctgatcc cgtcgctggt gcgcgacccg tcccggctgg tcgccaccaa
      421 cgcccgcctg ggccgtacgg ccagcatcgt cggagcgatc ggcgcgagcg ccggcctggc
      481 gttgcagcag agcgtgctcg acacccgcgg gttgctgcnn nnnnnnnnta ttacaacgaa
      541 gtcgaaccca acatcaagaa gggtgccgca ctggccttcg ccgcggccgc gatcgtgatc
      601 actcgcatgg ggttgcgcgg cgagaccgag caggaccgcc tgccgacgtc gctcgaatat
      661 gccgagaccc acgccccgcc cgtggtcgtc ggctcgatcg gtttcatggc gatccggggc
      721 gctgtcggct tcttcgtgtt catgctcgcc ttcacgctgc gtcaggagag cgaaccgaag
      781 tgggtgtacg gcgtggcgat cttcgtctac ggcgcaggcg cgttcgtggg caacctggtc
      841 gcaccggtgc tgcgcaagcg tttcagcgat cagcagttga tcgcgatcgc gatcgcgctc
      901 cccacgctgc caaccctgtt cggcatgctc ggtgtgtcgc gcgcgctgct gttggtgatt
      961 gcgacggcga tcggcgtgtc gaccacgctc gggcgccacg ggttcgacag cctgctgcaa
     1021 gatcgtgcct ccgcagcgtt gcgtggccgg gccgcggcgc gctacgagac ccggttccaa
     1081 ctggtgtggg ccatcggcgc gctgatcgcg accccgatct cgctgcctcc cgaagcgagc
     1141 atggccgtat tggccgcgct gtacattccg gcgctgtcgg tgttcctgcg cgcgtcaacc
     1201 gcggccaagg cgttcgaggc cgacgtgtcg ctcgacgtcg tcggtcgggc cggtgcccgc
     1261 ctcgccacgg ccgagcagtc cctgtccacc gagagctacg ccgtcgcggt gatcgacgcg
     1321 gctgccgcga tcgacctggc ccgtctctcc gacgccgacc tcagccggct gcaggaatgc
     1381 gtcgatctca accggttgcg cctcgccgcg ctggaacacc gcgagacggt cacccatgcc
     1441 gaagcgcagg aagcggtcgc gttggcccgc ctgctgctga cctctattcg ttctccgtcg
     1501 cctgagtgac cacgtaggtg ccgtcgcggc gagagatgcg cagggtgtag tcgtgctggg
     1561 tggaactatt gaagtagacc tcgaccgaca cgtcgaccgg tgggcgcttc tcgggcacct
     1621 tgccgaccac cagcagcgcg ccatcgtcac gcaccggctc gccttcggcg tcaaggtcga
     1681 tcgcgtcgtc gcgcacgtcc gcgaagcgca actcgatgtt gtcgcccatg tcggccaggt
     1741 tgcccaccac cgccacctgc gaaccggccg ggatggggct gccgctggtc ggtgccacgt
     1801 agaccacgcg cgccttggtg acgatggtgt cgtcggggcc gatcttcgag ccgannnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngagg gtgatgggct
     1921 tgccggctcc tgcagagtcg caggcgatcg tcgacaagat cagcgcaccg agcgcgacag
     1981 caagcaatgt ccgacggcga cggatcacgg tgccacccta accgccaccc accgacccgg
     2041 atcgcacgtt caacgatcga gggctcggcg cagcgctact gccatctgct cgcggtacgg
     2101 ggtgccggtc ggcagcacgt cgaccgcggt cagcgacgtg tgcacgtggc cagcggcacg
     2161 aatgtggtcg acggccaccc cggcagcggc gagcgcggcg gcgtaggcgt cgccctgatc
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnngc ggcgaggtgc gatcggcggg atcggcgtag
     2341 tggtcccaga accagtccat cgtcgccttg gtgagcatgt aaccctcggc gttggcgagc
     2401 atcgacggcc gtacgaccgt gccgtcggtg acgggggcga gcaacagctg cgccgcgatc
     2461 tgcggcccgc cttcgtcgcg ggcgcgctgg cagacgacgg cggccaggtt gccaccggcg
     2521 ctccaccccg cg
//
LOCUS       DAORNF010000512         2529 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_31042_length_2529_cov_1.966047, whole genome shotgun sequence.
ACCESSION   DAORNF010000512 DAORNF010000000
VERSION     DAORNF010000512.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2529
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31042_length_2529_cov_1.966047"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..901
                     /locus_tag="PLV68_16980"
     CDS             <1..901
                     /locus_tag="PLV68_16980"
                     /inference="COORDINATES: protein motif:HMM:NF037864.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HRE02996.1"
                     /translation="HAKLAYETVIAADLPEGFDEFVSRAMLAEERRGASRVETPEQEI
                     EPDGHGGYHLAFRPRLASEVANASLSLSTNLAVATLLRSHHTGLFRVMAEPDERAIRR
                     LRHTARGLGLVWPPDLSLRAFELTLTDAQAADVAAFQMAVRRSTGGAVYQPFVEGVVP
                     WHAAVAASYVHATAPLRRLADRYVVEAALAIAAGDAVPEPVEAAFATLPEVMRTTEGR
                     AAQVDRAAIDLIEAVVLHGREGAIFDAVVTDADERGARVQLLDPAVIARVNTQRVEMG
                     EVIRVRLVEADPQARQVRFERVN"
     gene            complement(938..>1521)
                     /locus_tag="PLV68_16985"
     CDS             complement(938..>1521)
                     /locus_tag="PLV68_16985"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HRE02997.1"
                     /translation="NPVGDSPYSIPPDNPFVGVTGYRPEIWALGLRNPWRFSFDRDTT
                     DLWIADVGQGDWEEIDVAWADEGNGRGANFGWSAFEGTHPFNGDVATDITGDEPITPI
                     YEYPHGEAGCSVSGGVRYRGLLHPALVGYYVYGDYCSGQVRALEIRDDRTAGREVVLG
                     RIGGISAVTEDASGELLVLSVSTSTVYTIASPG"
     assembly_gap    1522..1542
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1543..2256)
                     /locus_tag="PLV68_16990"
     CDS             complement(<1543..2256)
                     /locus_tag="PLV68_16990"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HRE02998.1"
                     /translation="MLAFGLITIAAGCGSDGAEGGPTVAPSVPLGTTSTSTAPTTTTP
                     PPSDPTATPNSTTDPGTTDPATTETVAPSTDAPNTGGNPPDPSTMQFVAIADLDAPVD
                     VAVRPSDNTLYAVSKDGYVVIIGDAGGGQRALDITGQVATGGEQGLLGLTFSQDGQRA
                     FIDLTNTSGDTEIREYDVEASGLFDVASERVLLTIAQPYSNHNGGQVRIGPDGYLYIG
                     MGDGGAANDPQRHASDPASL"
     gene            complement(2346..>2529)
                     /locus_tag="PLV68_16995"
     CDS             complement(2346..>2529)
                     /locus_tag="PLV68_16995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HRE02999.1"
                     /translation="ASLDHALWFHRPFRADEWLLYDQVAISTNAARGLAGGSIYRQDG
                     TLAITVVQEGLARVAQ"
BASE COUNT          344 a          833 c          922 g          409 t
ORIGIN      
        1 ccacgccaag ctcgcctacg agacggtgat cgcggccgac ctgccggagg gcttcgacga
       61 gttcgtcagc cgggcgatgt tggccgagga acgccgcggc gcgtcgcggg tcgagacgcc
      121 cgagcaagag atcgagccgg acggtcacgg tggctaccac ctcgccttcc ggccgcggct
      181 cgcatccgag gtcgccaacg cgtcgttgtc gttgtcgacc aacctcgccg tcgccacgct
      241 gctgcgttcg caccacacgg gtctgttccg ggtgatggcc gagccggacg aacgggcgat
      301 ccgccgcttg cgccacaccg cccgcggctt gggtttggtg tggccgcccg atctgtcgtt
      361 acgggcgttc gaactgaccc tcaccgacgc acaggcggcg gacgtcgcgg cgttccagat
      421 ggcggtgcgc cgctcgaccg ggggtgccgt ctatcagcca ttcgtcgaag gtgtcgtgcc
      481 gtggcacgcc gcggtcgctg ccagctacgt ccatgcgacc gcgccgttgc ggcggttggc
      541 cgatcggtac gtggtcgaag ctgccctggc gatcgccgcc ggcgacgcgg tgcctgagcc
      601 ggtcgaagcc gccttcgcga ccctgcccga ggtgatgcgt acgaccgagg gccgcgccgc
      661 ccaggtcgac cgggccgcga tcgatctgat cgaagccgta gtgttgcacg gtcgggaagg
      721 agcgatcttc gatgccgtgg tcaccgacgc cgacgagcgt ggggcgcggg tgcaattgct
      781 cgacccggcg gtgatcgcac gcgtcaacac gcagcgggtt gagatgggcg aagtgatccg
      841 cgtgcggctg gtcgaagccg acccgcaggc gcgccaggtg cggttcgagc gcgtgaactg
      901 acgggcgggg gcgaaatcgg ctcggtgagc gtgtcgatca gcccggcgag gcgatcgtgt
      961 acaccgtcga ggtcgacacg ctcagcacca gcaactcgcc tgacgcgtct tctgtgaccg
     1021 cgctgatacc gccgatccgg ccgagcacga cctcgcgccc ggccgtgcgg tcgtcgcgga
     1081 tctccaaggc gcgcacctga ccgctgcagt agtcgccgta gacgtagtag ccgacgagcg
     1141 ccgggtggag caggccgcga tagcgcaccc caccgctgac cgagcaaccc gcttcgccgt
     1201 gggggtactc gtagatcggt gtgatgggtt cgtcgccggt gatgtcggtc gcgacgtcgc
     1261 cgttgaacgg gtgcgttccc tcgaaggcgc tccagccgaa gttggcgccg cggccgttgc
     1321 cttcgtcggc ccacgcgacg tcgatctctt cccagtcgcc ttggccgaca tcggcgatcc
     1381 ataggtcggt ggtgtcgcgg tcgaagctga accgccatgg gttgcgcaac ccgagcgccc
     1441 agatctcggg gcggtatccg gtcaccccga cgaagggatt gtcgggcggg atgctgtacg
     1501 gcgagtcgcc cacggggttg gnnnnnnnnn nnnnnnnnnn nncaggctcg cggggtcgct
     1561 ggcgtggcgc tgcggatcgt tggccgcgcc gccgtcaccc atgccgatgt agaggtagcc
     1621 gtcggggccg atccgcactt ggccgccgtt gtggttgctg tagggctggg cgatggtgag
     1681 cagcacccgc tcgctggcga cgtcgaacag tcccgacgct tcgacgtcgt actcacggat
     1741 ctccgtgtcg ccgctggtgt tggtcagatc gatgaatgcc cgttgaccgt cctggctgaa
     1801 cgtcagcccg agcagtccct gctcgccgcc cgtggcgacc tggccggtga tgtcgagcgc
     1861 ccgctggccg ccgccggcgt cgccgatgat gacaacgtaa ccatccttgc tgaccgcgta
     1921 cagcgtgttg tcgctgggcc gcacggccac gtcgaccggg gcatcgaggt cggcgatggc
     1981 gacgaactgc atcgtcgacg gatcgggtgg gttgccgccc gtgttcggcg cgtcggtgct
     2041 cggcgcgacc gtctcggtcg tcgccggatc ggtggtaccc ggatcagtgg tggagttggg
     2101 ggtggcggtc gggtcagacg gcggcggcgt cgtggtggtc ggcgcggtcg aggtcgacgt
     2161 ggtgccgagc ggcacgctgg gcgcaactgt cggcccgccc tccgcgccgt cgctgccgca
     2221 accagcggcg atggtgatca ggccgaacgc cagcaccaac cgcgccagca gtgaaggtgg
     2281 cgtcgccagg cgattcggcc tcggccgggc cagagaacgg accggggtcg gtgcggtgcg
     2341 gcgcttcact gtgcgacccg cgccagacct tcctgcacca ccgtgatcgc cagcgtgccg
     2401 tcctgacggt agatcgaacc gccggccagc ccgcgggcgg cgttggtgct gatcgcgacc
     2461 tggtcgtaga gcagccactc gtcggcccga aaggggcggt ggaaccacag ggcgtgatcc
     2521 aggctcgcc
//
LOCUS       DAORNF010000513         2524 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_31131_length_2524_cov_1.982584, whole genome shotgun sequence.
ACCESSION   DAORNF010000513 DAORNF010000000
VERSION     DAORNF010000513.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31131_length_2524_cov_1.982584"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..805)
                     /locus_tag="PLV68_17000"
     CDS             complement(<1..805)
                     /locus_tag="PLV68_17000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRE03000.1"
                     /translation="MHEMGPSQRPARTRPLRVGMISPYSLSIPGGVQQQVMGLARELR
                     RMGYEVRVLGPCDGPPPATFVSPLGNSLPTAANGSIAPIAPDPIAQFRTIRILNDEDF
                     DVIHLHEPLAPGATETALFMNTAPIVGTFHAAGTSKGYRTLDRPLRALAGNLAHRVAV
                     SKDAYGLVYDAIGGDYEILFNGVELDVYRDTPATDTERPDTERPDNERANNERANTER
                     SGNERDGLAGLDLEAFVEGAHPHHIALHFHFVNFEYPGRHRAAADQAIRL"
     gene            complement(852..>1782)
                     /locus_tag="PLV68_17005"
     CDS             complement(852..>1782)
                     /locus_tag="PLV68_17005"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRE03001.1"
                     /translation="RQTFVNDMVDRRRLTNAVGLNSASFNLARMIGPAVAGWLIAAAG
                     SGARATGWVILLNAASYAAVVISLFLIRASELDPVPRLVAAKGQLIEGVRYVRHRADI
                     MMVLAIVFCAGTFGFNFQMTTALMATEVYDKGAGEYGNLGSILAVGSLVGSLFAARRV
                     VTRQRLVVVAGVVFGGIEMIAGLMPSYLTFALLLPLCGVTALTMVTAANAYVQTATDA
                     VVRGRVMALYMMIFMGGTPLGAPALGWVSDHFGARWTLIGGGLLTAVGTLAAAAFFGP
                     RQGVSASTAWHAGPRLLRSVRQTNETVEEIASV"
     assembly_gap    1783..1792
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1793..2161)
                     /locus_tag="PLV68_17010"
     CDS             complement(<1793..2161)
                     /locus_tag="PLV68_17010"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRE03002.1"
                     /translation="MSGTFDSLKIRNYRVFAVGTLISNTGTWMQRVAQDWLVLKLTDG
                     SAGALGITTGLQFLPTLLFSATAGVCADRYSKRKLLVLAQFAMGISAAVLGVLAVTGT
                     ARTWHVYVIAFLFGTASAFEV"
     gene            complement(2336..>2524)
                     /locus_tag="PLV68_17015"
     CDS             complement(2336..>2524)
                     /locus_tag="PLV68_17015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03003.1"
                     /translation="ASAWVRWLDPAVTDAEALQHLLVPAPDGLLTMHPVSSEVNNARN
                     AGSHLIEPVSPAAPNRLL"
BASE COUNT          436 a          850 c          842 g          386 t
ORIGIN      
        1 ccagcctgat cgcctgatcg gcggcagcgc ggtggcggcc gggatattcg aagttcacga
       61 aatgaaaatg gagggcaatg tgatgcggat gcgcgccctc gacaaaggcc tcgagatcaa
      121 gcccggccag accgtcacgc tcgttgccgg aacgctcggt gttggcacgc tcgttgttgg
      181 cacgctcgtt gtcgggacgc tcggtgtcgg gacgctcggt gtcggtggcc ggggtgtcgc
      241 ggtacacgtc gagctcaacc ccgttgaaca ggatctcgta gtctccgccg atcgcgtcgt
      301 agaccaggcc gtacgcgtcc ttggagacgg ccacacggtg ggccaggttg cctgccaatg
      361 cgcgcagcgg ccgatcgagg gtgcggtacc ccttggaggt gcctgcggcg tgaaacgtgc
      421 cgacgatcgg cgccgtgttc atgaacagtg ccgtctcggt cgccccgggc gccagtggtt
      481 catgcaggtg gatcacgtcg aagtcctcgt cgttgaggat gcgaatcgtg cggaactggg
      541 cgatcggatc gggcgcgatc ggcgcgatcg acccgttggc cgcggtgggc agactgttgc
      601 cgagcggact gacgaaggtg gccggtggcg gtccgtcgca gggaccgagc acccgcacct
      661 cgtaccccat ccggcgtagc tcacgggcca ggcccatcac ctgctgttgc acgccgccgg
      721 ggatcgacag gctgtagggc gagatcatcc cgacccgcag cggccgcgtc cgcgccgggc
      781 gctggctggg gcccatctcg tgcatcgggc gaccctatcg ccacccaaga gaacgtcccg
      841 cgccagcgcg ctcagaccga ggcgatctct tcgaccgtct cgttggtctg gcgcaccgac
      901 cgcaacaggc ggggaccggc gtgccaggcg gtcgaggccg agaccccctg gcggggcccg
      961 aagaacgcgg ctgccgccag cgtgccgacc gccgtcagca ggccaccgcc gatcagcgtc
     1021 cagcgggcgc cgaagtgatc ggacacccag cccagcgcgg gagcccctaa cggggtgcca
     1081 cccatgaaga tcatcatgta caacgccatg acccgcccgc gcaccaccgc gtcggtcgcg
     1141 gtctgcacgt aggcgttggc cgcggtgacc atcgtcaacg cggtcacacc gcacaacggc
     1201 agcaagaggg cgaaggtgag atagctcggc atcaggccgg cgatcatctc gatccccccg
     1261 aacaccacgc ctgcgacgac caccagccgc tgtctggtga ccacgcgccg agccgcgaac
     1321 aacgagccga ccagtgaacc gacggccagg atcgaaccga ggttgccgta ttcgccggcg
     1381 cccttgtcgt agacctccgt cgccatcagc gcagtggtca tctggaagtt gaacccgaag
     1441 gtgccggcgc agaacacgat cgccagcacc atcatgatgt cggcacggtg gcgcacgtag
     1501 cgcacgcctt cgatcaactg gcccttcgct gccaccagtc gtggcaccgg gtcgagttcg
     1561 ctggctcgga tgaggaacag cgagatcacc accgccgcgt aacttgctgc attgagcagg
     1621 atcacccacc ccgtcgcccg ggccccggag ccggccgccg cgatcaacca gcccgccacc
     1681 gccgggccga tcatgcgggc caggttgaac gaagccgagt tgagaccgac ggcgttggtg
     1741 agacggcggc ggtcgaccat gtcgttgacg aaggtctggc ggnnnnnnnn nncacctcga
     1801 atgcgctcgc ggtgccgaac aggaacgcga tcacgtagac gtgccatgtc cgcgcggtgc
     1861 cggtcaccgc cagcacgccg aggactgcag ccgagatgcc catcgcgaac tgggccagca
     1921 ccagcagttt gcgcttcgag taccggtcgg cacacacacc tgcggtggcc gagaacagca
     1981 gcgtcggcag gaactgcagg ccggtggtga tgccgagcgc cccggcgctg ccgtcggtca
     2041 gcttgagcac caaccagtcc tgtgcgacac gctgcatcca cgtgccggtg ttggagatca
     2101 gcgtgcccac ggcgaagacg cggtagttgc gaattttgag cgaatcgaag gtgccgctca
     2161 tgagtttgcg gggcgcacgg tcacaacctg cgcacctttc acccgcctgc tcacctgcct
     2221 gctcacctgc cggttcagct gcccaccgtc gtcgttcaca aaccgttagc ttactgaact
     2281 atctagctcc acgagcgtca tgtttcgatc cgccgccaga tcggagcgag gcgggtcata
     2341 acaggcggtt cggcgcggcc ggtgacaccg gttcgatgag gtggctgccg gcgttgcggg
     2401 cgttgttcac ctcactcgac acgggatgca tcgtcagcag gccgtccggg gccggcacca
     2461 acaggtgttg cagcgcctcg gcgtcggtga ccgccggatc gagccagcgc acccacgcgc
     2521 tggc
//
LOCUS       DAORNF010000514         2524 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_31132_length_2524_cov_1.940462, whole genome shotgun sequence.
ACCESSION   DAORNF010000514 DAORNF010000000
VERSION     DAORNF010000514.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31132_length_2524_cov_1.940462"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..459)
                     /locus_tag="PLV68_17020"
     CDS             complement(<1..459)
                     /locus_tag="PLV68_17020"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRE03004.1"
                     /translation="MNAPERAPWDGDELPQGDAKAAAVRQMFDTVAPRYDLINRIMTF
                     RLDVRWRRIAVKRLGLPPGATVADLASGTGDLCIDLMRAGHRPISVDFSFGMLAGDRS
                     GAPRVQADILRLPFATASLDGATCGFALRNLVDLPAFFAELGRAVKPGGRI"
     gene            complement(456..683)
                     /locus_tag="PLV68_17025"
     CDS             complement(456..683)
                     /locus_tag="PLV68_17025"
                     /inference="COORDINATES: protein motif:HMM:NF024591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03005.1"
                     /translation="MVTGRPSERWGNEVVAVVRMRDGHSADEDSLRAEAERHIARYKL
                     PKGFVFVDAIVRSPSGKADYRWAKELAAASA"
     gene            complement(911..2098)
                     /locus_tag="PLV68_17030"
                     /pseudo
     CDS             complement(911..2098)
                     /locus_tag="PLV68_17030"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
     gene            complement(2098..2517)
                     /locus_tag="PLV68_17035"
     CDS             complement(2098..2517)
                     /locus_tag="PLV68_17035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HRE03006.1"
                     /translation="MIGEHLGWSDYLEITQERVNQFAEATGDHQWIHVDPERAAKESP
                     FGGPIAHGYLTLSLGPVLYPQVVAFTGFKMGVNYGCNKVRFMSPVPVGGRLRLGVTLV
                     AVDDIPGGVSSTLEFVFEVEGAAKPSCVAEVIFRSYV"
BASE COUNT          394 a          852 c          892 g          386 t
ORIGIN      
        1 gatccgccca ccgggcttga cggcgcggcc cagctcggcg aagaaggccg gcaggtcgac
       61 caggttgcgc agcgcgaacc cgcacgtcgc gccgtcgagg ctggcggtag cgaacggcaa
      121 ccgcaggatg tcggcctgca cgcggggcgc gccgctgcga tcgccggcca acatgccgaa
      181 gctgaagtcg accgagatcg gccggtgccc ggcgcgcatc aggtcgatgc acaggtcacc
      241 ggtgccgctg gccagatcgg caaccgtggc gccggggggc aaccccagcc gcttgacagc
      301 gatgcgccgc cagcggacgt cgaggcgaaa cgtcatgatc cgattgatca ggtcgtaccg
      361 cggcgccacc gtgtcgaaca tctggcgcac cgcggcggcc ttggcgtcac cctggggaag
      421 ctcgtcgcca tcccacgggg cgcgctcggg cgcgttcatg cgctggccgc tgcgagctct
      481 ttggcccacc ggtagtcggc cttgccggac ggggagcgga cgatcgcgtc gacgaacacg
      541 aaccccttcg gcagcttgta gcgggcgatg tgccgctcgg cttcggctcg caggctgtcc
      601 tcgtcggcgc tgtgtccgtc gcgcatgcgc accacggcga cgacctcgtt gccccatcgc
      661 tcgctgggcc gaccggtgac cacgcagtcg tagacgcccg ggtgggcctt gatcgcggcc
      721 tcgacctctt cggcaaagat cttctcgccg ccgctgttga tggtgacgct gtcgcggccg
      781 tgcagttcga ccacggaatc ggcccgcagc ctggcccggt cgcccggcac ggcgtggggg
      841 atggcgctgt ttgggccgcc ggcgccgtct tggcttcgtc gcccaggtaa ccgagcgcca
      901 gcctcccgct cttggccaac caacccaact cgtcgtcacc cggcgcgagc tcgcggtcga
      961 gctcggcgga caacacgtgg ttgcccggcg cgagcgggaa ggtgccggtc gaagccgacg
     1021 cacccgccga gacctggctc atctgcccgc cggcttcgga cgagccgaga ccgtcgacga
     1081 tcagcgtcgt cggcaggtgg gcgatgaact cgtgtttgag gttgacgctg agcggggcac
     1141 cgccgctgag gatcacggtc aggctcgaca ggtcgtacct gtgctggtcg agcccgtcga
     1201 gcaacgggcg ggcgaacgcg tcgccgacga tcagcaggaa gttgagccgc tcgcgttcga
     1261 tgatgcccca cacgtcggcc gggtcgagcc gttcgggatg gctctggatg aagaccgtgc
     1321 cgccggtgaa ccaggtgcgg aaagcgatcc agtgacccgc cccgtgcatg aacgggggag
     1381 cgatcaggct ctgcaggttg tcggccgcgg cagcgacgtg atcgtccaac gacagggccg
     1441 ttgctgacgc accgaagcaa ccgatcgccg cgtcggcgtt gcgccacagc acacccttgg
     1501 gcatgccggt ggtaccgccg gtgtagagga tgtacaggtc gtcgggcgac gcgggcgcgg
     1561 gcgcagtcgt cggcgccgac gccaacgcgt cctcgtacca gacagcgccc ggcagcagcg
     1621 gattgcccga ctcgtcggcc acctggagga tcacccgcac cgacggggtg tcgggcagta
     1681 cttcggccag cgtgggtgcg aacgcggaat ggacgaccac ggcggccgcc ccggagtcgg
     1741 tcagcaggta gcgcagctcc tcggcgacgt accggtagtt gacgttcatt ggtgcgaccc
     1801 gcgccttgaa cgccccgagc atcgactcca agtactcgtt gccgttgtgg aggtagatgg
     1861 cgagatggtc ctggccgctc tcccagttct gcagatcgcc gcgctcggta cggcagccca
     1921 ggccttggcc gacgaggtag ttggcgagtt gccgggtgcg atcggtgacg tcctgccacg
     1981 tcaggcgccg gtcacgaaag accaggcaca ggtggtcggg gcggctcgcc gcgatcgcct
     2041 cgtggatcgc cccgacgctg agctctggtg cgtcggcggc gccgctgatg gccgtcatca
     2101 gacgtagctg cggaagatga cctcggccac gcacgacggc ttggcggcac cttcgacttc
     2161 gaacacgaac tcgagcgtcg aggacacgcc gccggggatg tcgtcgaccg cgaccagcgt
     2221 cacgcccagg cgcagacgtc cgccgaccgg caccggcgac atgaagcgca ccttgttgca
     2281 gccgtagttg acgcccatct tgaaaccggt gaaggcaacg acctgcgggt acagcaccgg
     2341 cccgagcgac agcgtgaggt aaccgtgggc gatcggacca ccgaaggggc tctccttggc
     2401 cgcccgctcg ggatcgacat ggatccactg atggtcgccg gtcgcctcgg cgaactggtt
     2461 gacgcgctct tgggtgatct cgagatagtc gctccagccg aggtgttccc cgatcagagc
     2521 tttc
//
LOCUS       DAORNF010000515         2521 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_31188_length_2521_cov_2.096513, whole genome shotgun sequence.
ACCESSION   DAORNF010000515 DAORNF010000000
VERSION     DAORNF010000515.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2521
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31188_length_2521_cov_2.096513"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="PLV68_17040"
     CDS             complement(<1..198)
                     /locus_tag="PLV68_17040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03007.1"
                     /translation="MLRAQPMIFLADVEAGSRWFQDVLGLTSGHGGTEYEMLMDGDTL
                     VAQLHRWDAHEHPHVGDPQAPE"
     gene            complement(260..622)
                     /locus_tag="PLV68_17045"
     CDS             complement(260..622)
                     /locus_tag="PLV68_17045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03008.1"
                     /translation="MLTNPTINGSPDAVPAVDGGASVVGAEAGVEEAAVEELTGAEVD
                     VVAAVVVAASAVVDGAFVEAELGDDASSSDPHAAVSRASAVTTAAAGRARRRDLTNVD
                     MSPPDCRWRRECAGGHIS"
     gene            621..1703
                     /locus_tag="PLV68_17050"
     CDS             621..1703
                     /locus_tag="PLV68_17050"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRE03009.1"
                     /translation="MEAGPLAQPAIGDSARAAVSYINKYMSGVDGRPIELDQCFVDGS
                     PESSAKCATQFVANGTQVVAMALDFTDAALLPVLAEAGITVIGQQPFSEADYTTETAH
                     YFAGSQIGSAYGLALYLRDEIKPKSVSVLTNGSAGAKSRLETFIVPALEQAGVTDVNT
                     VVIDPSQPDFSVIVAAALSNSPDLIFGFLQESDCTKLVTTARQLNFTGDIVAGNCAEF
                     VTDSGDAAEGVYVVGDMYNPYDDTKATPRAKEELKLFLDAMEEFAPDRSIGVFTQFMF
                     AGIMNLRNLMVGVGADNYSSEAVEAAMAATDGEPSFMAESYGCAPEPVPGYSVCGAST
                     LIFQVRDGKLVQASDWIFGPDLWADN"
     gene            1703..>1946
                     /locus_tag="PLV68_17055"
     CDS             1703..>1946
                     /locus_tag="PLV68_17055"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRE03010.1"
                     /translation="MTVGSPGGDAGPNPVPSIPVLSVRALSVSYGGVNSLTDVDLSVG
                     AGEIVGLIGPNGAGKTTMIDALSGFVRHKQGWIELGG"
     assembly_gap    1947..1959
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1960..2485
                     /locus_tag="PLV68_17060"
     CDS             <1960..2485
                     /locus_tag="PLV68_17060"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRE03011.1"
                     /translation="AHRRARAGLTRTFQSTESFAPLTVRANLGAAAEAHRGRRAQREI
                     SQRVDAVLAALGLDAVADSFPPDLPYGTQKLVGLGMALIVEPSVVLLDEPAAGLDAAS
                     VAILKQRLRSLREQGLALLLVDHDMDVLFDVCDHVAVLDFGQIIAAGTPDMVRADERV
                     RAAYLGEAAEAAVA"
BASE COUNT          394 a          839 c          853 g          422 t
ORIGIN      
        1 ctctggcgcc tgcgggtcgc cgacgtgggg gtgctcgtgc gcgtcccagc ggtgcaactg
       61 ggcgacgagc gtgtcgccgt ccatcagcat ctcgtactcc gttccgccgt ggccggacgt
      121 cagccccagc acgtcctgaa accaccgcga ccctgcttcg acgtcggcga ggaagatcat
      181 cggttgcgcc cgcagcatgg tccgatcgta ctgccgcgag gaaacggtgt cgattgacag
      241 cttgcgagtg aatgcttact tagctaatgt gacccccggc gcactccctg cgccaccgac
      301 aatccggggg actcatgtca acgtttgtga ggtcgcgacg ccgcgcccgt cctgctgccg
      361 ccgtggtgac ggcgctggct ctactcaccg ccgcgtgcgg gtctgacgac gacgcgtcgt
      421 cgccgagttc ggcttcgacg aatgcaccgt cgacgacggc cgacgcggca acgacgacgg
      481 ccgcgaccac atcgacctct gcgccggtga gctcttcgac cgctgcttcc tccacgcccg
      541 cttcggcgcc gaccaccgac gcgccgccgt cgacggccgg gacagcgtcg ggtgagccgt
      601 tgatcgtcgg gttcgtcaac atggaggcag ggccactcgc gcaaccggcg atcggcgaca
      661 gcgctcgcgc ggcggtgtcg tacatcaaca agtacatgag tggcgtcgat ggtcggccga
      721 tcgagctcga tcaatgcttc gtcgacggtt cacccgaatc gtcggccaag tgcgcgacgc
      781 agttcgtcgc caacggcacg caggtggtgg cgatggcgct cgacttcacc gacgccgcgc
      841 tgttgcccgt gctcgccgag gcaggcatca ccgtcatcgg ccagcagccc ttcagcgaag
      901 cggactacac gaccgagacg gcgcactact tcgccggcag ccagatcggg tcggcctacg
      961 gcttggcgct gtatctgcgt gacgagatca agccgaagtc ggtctccgtt ctgaccaatg
     1021 gctcagccgg tgccaagtcg cggttggaga cgttcatcgt gcccgcgctg gaacaggccg
     1081 gggtgacgga cgtcaacacg gtcgtgatcg acccgagcca gcccgacttc agcgtgatcg
     1141 tcgcggcggc gttgtcgaat tcgcccgact tgatcttcgg cttcctgcaa gagtcggact
     1201 gcaccaagct ggtcaccacg gcccgccagt tgaacttcac cggcgacatt gtggccggca
     1261 actgcgccga gttcgtcacc gactcaggcg acgcggccga gggcgtctac gtcgtcggcg
     1321 acatgtacaa cccgtacgac gatacgaagg cgaccccgcg ggccaaggaa gaactgaagc
     1381 tgttcctcga cgcgatggag gagttcgccc cagaccgctc gatcggcgtg ttcacccagt
     1441 tcatgttcgc cggcatcatg aacctgcgca atctgatggt cggcgtgggg gccgacaact
     1501 acagctccga agcggtggaa gcggcgatgg ccgccaccga tggcgaaccg tcgttcatgg
     1561 ccgagtcgta cgggtgcgcg cccgagccgg tgccgggcta ctcggtgtgc ggggcgagca
     1621 cgctgatctt ccaggtgcgc gatggcaagc tcgtccaggc ctccgactgg atcttcggac
     1681 ccgatctgtg ggctgacaac tgatgacggt cgggtcgcct ggtggcgatg cggggccaaa
     1741 cccggtgccg agcatcccgg tgctgagcgt ccgtgcgctg agtgtctcct acggcggcgt
     1801 caactcgctg accgacgtcg acctcagtgt cggcgccggg gagatcgtcg ggctgatcgg
     1861 gcccaacggt gctggcaaga cgacgatgat cgacgccctg agtgggttcg tgcgccataa
     1921 gcaggggtgg atcgaactcg gcggcgnnnn nnnnnnnnnc gcgcaccgtc gggcccgcgc
     1981 cgggctgacc cgaacctttc agtcgaccga gtcgttcgcg cccttgacgg tgcgcgccaa
     2041 cctgggcgcg gccgccgagg cgcaccgcgg ccggcgtgcg cagcgcgaga tcagccagcg
     2101 agtcgacgcg gtactcgccg cactcgggct cgacgcggtc gccgactcgt ttccacccga
     2161 tctgccgtac ggcacgcaga agttggtcgg gctgggcatg gcgctgatcg tcgagccgtc
     2221 ggtcgtgctg ctcgacgaac cggccgccgg tctcgacgcc gcgtcggtgg cgatcttgaa
     2281 gcaacgcctg cgctcgctgc gcgagcaagg gctggcgttg ttgctggtcg atcacgacat
     2341 ggacgtgctg ttcgacgtgt gcgaccacgt cgcggtgctc gatttcggcc agatcatcgc
     2401 cgcgggcacg cccgacatgg tgcgggccga cgaacgggtc cgtgctgcct acctgggcga
     2461 agcggccgag gctgcggtgg cctgatgacg ccagacgccg cggcatcgct cgcaaccgct
     2521 t
//
LOCUS       DAORNF010000516         2520 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_31210_length_2520_cov_1.607708, whole genome shotgun sequence.
ACCESSION   DAORNF010000516 DAORNF010000000
VERSION     DAORNF010000516.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31210_length_2520_cov_1.607708"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>448
                     /locus_tag="PLV68_17065"
     CDS             <1..>448
                     /locus_tag="PLV68_17065"
                     /inference="COORDINATES: protein motif:HMM:NF036525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03012.1"
                     /translation="EISAAARALQGDAAILAQNAEELLSDASYVAAIDGIAKEDLLYE
                     IIQEVHEVWAANLQRSEILQGDPLTRIWVFVNYNVIGNASNLVNSAVFFRDFGALTGR
                     RREHILALRDVHDNTLRELIREGQADGSIRADVPAEIREQYATPPVA"
     assembly_gap    449..476
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    713..862
                     /estimated_length=150
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            886..>1364
                     /locus_tag="PLV68_17070"
     CDS             886..>1364
                     /locus_tag="PLV68_17070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03013.1"
                     /translation="MPAPGDPVPVASLGVAGPYGMTLYTTRFDEVLAMLGTATGHEPI
                     AISYHFPGTGTLVRECAARGPDGINIFLVEYDPSRHRCCIREGSDATVSEIAAVGFVV
                     DDLDASLAQHTEALGASVYMNEIFGGPAVERMNALAPGAQLHVAFLRGAEPGSARVEL
                     "
     assembly_gap    1365..1374
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1508..1879)
                     /locus_tag="PLV68_17075"
     CDS             complement(1508..1879)
                     /locus_tag="PLV68_17075"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRE03014.1"
                     /translation="MYVTSHVQGIHHVGLLVDDLDATLPFYTEVLGIPRNRDRPDFAV
                     RGAWLDLNDRQQLHVFEGPTPADGGQHVALQVTDLDGLIERLTSLGVKVRGFPREGPF
                     TQAVVFDPAGNRVELYAGPSA"
     gene            complement(1908..>2520)
                     /locus_tag="PLV68_17080"
     CDS             complement(1908..>2520)
                     /locus_tag="PLV68_17080"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRE03015.1"
                     /translation="PALDDDHWAPLFKEVEARGLSLNFHTGFSDYTEEEFVGQLGKDM
                     TRAGYAKSTALQQLGLAETLAELTTSEVCIKYPDMKMVIVESGVGWLRYFLELLDWQW
                     VNSGAQNAYPHRELPSFYFKRQIYGSFWFERESVSRIADLYQDNLMFETDFPHPTSLS
                     PGPISSSSTPREMLDHSLGGLDESIQRKIMYENAAKLYHVKGV"
BASE COUNT          422 a          789 c          735 g          386 t
ORIGIN      
        1 aggagatctc ggccgcggcg cgcgccctgc agggcgatgc ggcgatcctg gcgcagaatg
       61 ccgaggagct gctctccgac gcttcctatg tcgccgccat cgacggcatc gcgaaagagg
      121 atctcctcta cgagatcatc caagaagtcc acgaggtgtg ggcagcaaac cttcagcgca
      181 gcgagatcct gcagggtgat ccgctgacca ggatctgggt gttcgtcaac tacaacgtca
      241 tcggcaatgc atctaacttg gtcaactcag ccgtcttctt ccgagacttc ggagcgctca
      301 ccggtcgtcg ccgcgagcac attctcgccc tacgcgatgt gcacgacaac acgctccgcg
      361 aactgatccg cgaaggccag gccgacggct cgatccgcgc cgacgtgccc gccgagatcc
      421 gcgagcagta cgcgacgccg cccgtggcnn nnnnnnnnnn nnnnnnnnnn nnnnnnagcc
      481 ccgacggtgt gctgaccccc gaggcggtcg cggaagcgta cgcagacatg gccgtggcaa
      541 gcctggccgt caagcccggc ctcctcgaaa cggcgcgtac gcgggccaag gccgcactcg
      601 ctgccgaagc tgcgctcgcc gcccaatagt cagctccaac ggcgctgtgg gacttcgtcg
      661 ttgaccggac cggatacagg ccgtatcatc aaacgaacga ttggtggaag gannnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      841 nnnnnnnnnn nnnnnnnnnn nnccgacggt ggcgggatcc gcctggtgcc agcgccgggc
      901 gacccggtgc cggtcgcgtc tctgggagtc gccggcccgt acgggatgac gctctacacc
      961 acgcgcttcg acgaggtgct cgcgatgctg ggcacggcca ccggccatga gccgatcgcc
     1021 atctcgtacc actttcccgg caccggaacg ctggtccgcg agtgcgccgc ccgaggaccc
     1081 gacggcatca acatctttct cgtcgagtac gacccctccc gccaccgatg ctgcatccgc
     1141 gaaggaagcg acgccaccgt cagcgagatc gctgcggtcg gcttcgtcgt cgacgacctc
     1201 gacgcctcgc tcgctcaaca caccgaagcc ttgggcgcca gcgtctacat gaacgagatc
     1261 ttcggtggtc ccgcggtcga gcggatgaac gccctcgccc ccggcgccca gcttcacgtc
     1321 gcgttcttgc gcggcgccga accaggcagc gcccgggtcg aactnnnnnn nnnncggcaa
     1381 gggccaggtg accggagtca gcgcgatttg ccgggtgccc gagaccgtgc cgaccaccac
     1441 gatctcgctc gtccccggtg ccaccctgga tcgtcgtccg atctgacgcg acgagacgac
     1501 ggtcgcgtca ggccgatggt ccggcataca gctcgacacg attgccggcg ggatcgaaca
     1561 caaccgcctg cgtgaacggc ccctcgcgcg ggaacccgcg aaccttcacc cccagcgagg
     1621 tgagccgctc gatcaacccg tcgaggtccg tgacctggag cgcgacgtgc tggcctccat
     1681 cggccggcgt cgggccttcg aacacatgga gctgctgccg atcgttgagg tcgagccacg
     1741 cgccgcgaac ggcgaagtcc gggcgatcgc ggttgcgggg gatgccgagc acctcggtgt
     1801 agaacggcaa cgtcgcgtcg agatcgtcga ccagcagccc gacgtggtga attccctgca
     1861 catggctcgt gacgtacatc accacctcct ctccgcaaag tcgtccatca gacgcccttg
     1921 acgtggtaca gcttggcggc gttctcgtac atgatcttgc gctggatcga ctcgtcgagc
     1981 ccgccgagcg agtggtcgag catctcccgc ggcgtcgacg aggacgagat cggtccaggc
     2041 gacaagctgg tgggatgcgg aaagtccgtc tcgaacatca gattgtcctg atagaggtcg
     2101 gcgatgcgcg agaccgattc ccgctcgaac cagaacgaac cgtagatctg ccgcttgaag
     2161 tagaagctcg gcagctcacg atgggggtac gcgttctggg caccggagtt gacccattgc
     2221 cagtcgagta gttcgaggaa gtaccgcaac catcccacgc cgctctcgac gataaccatt
     2281 ttcatgtcgg ggtacttgat gcacacctcc gatgtggtca gctcggccag tgtctcagcc
     2341 aggccgagct gctgaagggc ggtcgacttg gcgtaaccgg cacgggtcat gtccttgccg
     2401 agctgtccga cgaactcctc ttcggtgtag tcgctgaaac cggtgtggaa gttgagcgac
     2461 aacccgcggg cctcgacctc tttgaacaac ggtgcccagt gatcgtcgtc gagagccggc
//
LOCUS       DAORNF010000517         2518 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_31243_length_2518_cov_1.911896, whole genome shotgun sequence.
ACCESSION   DAORNF010000517 DAORNF010000000
VERSION     DAORNF010000517.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2518
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31243_length_2518_cov_1.911896"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="PLV68_17085"
     CDS             complement(<1..182)
                     /locus_tag="PLV68_17085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03016.1"
                     /translation="MSRSIGRALRRFALPFALVAALSGGSVATAANKGDKIALEPTIS
                     FCAALSGCEESRTWMM"
     gene            complement(202..486)
                     /locus_tag="PLV68_17090"
     CDS             complement(202..486)
                     /locus_tag="PLV68_17090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03017.1"
                     /translation="MSLKSRIGWLVNGSANTHHALTQLGEDLRALQAKVDGLAERLAT
                     LEFAATVAPGRADVERLDEAIESMRVQLRVVTDDLGDRVGAVAGLLNQRR"
     gene            complement(503..>2518)
                     /locus_tag="PLV68_17095"
     CDS             complement(503..>2518)
                     /locus_tag="PLV68_17095"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRE03018.1"
                     /translation="GMVAVMGDLAGLTTDEVAITCWARTSDGIANDGLAPGEADLLRL
                     GPVSESVVVFGVDAAPALGWLGAQMVAEGSTSTAASSPPTPTARWLDRCVQRFHGSIA
                     PPGCVHAVGWQTVADATTPLPVVVDLAAIDRGEPWHVSFMDTPARLRLSAAPSIAALV
                     EAGWSQCAGQPTPLRLPGGIPVAGAVRQVTAINLVNHDPADSLPPEPFGPDHSRFVAW
                     LESSAPYEADVGRYWAALRQDRADLQAAFPQPHGADAVRLRHWGDVAWRLEPLTCLLW
                     GYRDRGGLAQSSQVSPSDGADDMAVRRPGGFNVLGYLNFDQSLGHIARRIVAALDAAG
                     VGVAAINNARTRGAPRAQPYTTTTEPRFAANIVVANADQFAFVVADHGDMLRDRYTIG
                     YWFWELDEVPPSMAAALADVDEVWTGSEFVADAFRRITNQPVHCMPLPVEEPSPSAPD
                     RARFGVPNDAFVFVTTFDHRSVPERKNPFGAIEAFRRAFADGEGPVLWVKSINGDQLW
                     RSHERVLLAAAGRSDIVVTDAHLSHADQMAVLAASDCLVSLHRSEGLGLHCAEAMWLG
                     KPVIATRYSGNLDFMDDTCAALIDYTLTPVRHGEGVYPETSQWADPDLDQAAAWMRRL
                     VADRDVVTALGAAARAKMKGQMSLAEAGHLMASRVGAASRRSSRPAI"
BASE COUNT          454 a          874 c          824 g          366 t
ORIGIN      
        1 atcatcatcc acgtccgcga ttcctcgcac cccgacagcg cggcgcagaa ggagatcgtc
       61 ggttccaacg cgatcttgtc gcccttgttg gcagccgtag ccaccgaccc gcccgacagc
      121 gcggcaacca gggcaaaggg gagagcaaag cgccgcaggg cgcggccgat cgaacgagac
      181 atcgagggag tcatcggctg ctcagcggcg ctggttgagc aacccggcca cagcgcccac
      241 gcggtcgccc aggtcgtcgg tcaccacgcg caactggacg cgcatcgact cgattgcctc
      301 gtcgagccgt tccacgtctg cccggccggg agcgacggtg gcggcaaact ccaacgtggc
      361 caggcgttcg gccaatccat cgaccttggc ctgcagcgcg cgcaggtcct caccaagctg
      421 ggtcagcgcg tggtgcgtgt tggcgctgcc gttgaccagc caaccgatgc gtgacttgag
      481 cgacatatgc ggattccttt cgtcaaatcg ccggtcgcga cgaccggcga gaagccgcac
      541 ccactcgact ggccatcaag tgaccagcct ccgccaacga catctgcccc ttcatcttgg
      601 cacgcgccgc ggcaccgaga gcggtcacca cgtcgcggtc ggcgacgagt cggcgcatcc
      661 acgccgctgc ctggtcaagg tccgggtcgg cccactgcga cgtttcggga tacacgccct
      721 cgccatggcg cacgggcgtg agcgtgtagt cgatgagggc cgcgcaggtg tcgtccatga
      781 agtcgaggtt gcccgagtag cgggtggcga tgaccggctt gcccagccac atcgcctcgg
      841 cgcagtgaag cccgagccct tcgctgcgat gcaagctcac cagacagtcg ctcgccgcaa
      901 gcaccgccat ctggtccgca tgggacagat gagcatcggt gaccacgatg tcactccgcc
      961 ccgcagcagc gagcaggacg cgttcgtgtg aacgccagag ctgatcgccg ttgatcgact
     1021 tcacccacaa caccggacct tcaccatcgg cgaatgcccg cctgaacgcc tcgatagccc
     1081 cgaatgggtt cttacgctcg ggcacactgc ggtggtcgaa ggtggtgacg aacacgaacg
     1141 cgtcgttcgg cacaccgaat cgggcccgat cgggtgccga cggcgatggc tcctcgaccg
     1201 gaaggggcat gcaatggacc ggttggttgg tgatccgccg gaaggcatcg gcgacgaact
     1261 cgctcccggt ccagacttcg tccacatccg caagagccgc ggccatcgac ggtggcacct
     1321 cgtcaagctc ccagaaccag tagccgatcg tgtagcggtc gcgcagcatg tcaccgtggt
     1381 cggccacgac gaacgcgaac tgatcggcgt tggccaccac gatgttcgcg gcaaagcgcg
     1441 gctcggtcgt ggtcgtgtac ggctgggcgc gcggggcgcc gcgggtacgg gcattgttga
     1501 tcgctgcgac gccgacgcct gccgcgtcca gcgcggcgac gatacggcga gcgatgtgac
     1561 cgagactctg gtcgaagttc aagtaaccca acacgttgaa tccgccgggc cgacggacgg
     1621 ccatgtcgtc ggccccatcc gagggcgaca cttggctgct ctgggcaaga ccgccgcgat
     1681 cccgatagcc ccacaacaga caggtaagcg gttcgaggcg ccaggccacg tccccccagt
     1741 gacgcaggcg gaccgcgtca gcgccgtgcg gctgtggaaa cgcagcctgc aagtcggcac
     1801 ggtcctggcg cagcgccgcc cagtagcgac cgacgtcggc ttcgtatgga gccgacgatt
     1861 ccagccaggc gacgaagcgc gagtgatccg gaccgaacgg ctcgggtggc aatgagtcgg
     1921 ccggatcatg gttcacgagg ttgatggcgg tcacctggcg cactgcgccc gccaccggga
     1981 tgccgcccgg cagccgcagc ggtgtcggct gaccagcaca ctgcgaccat ccagcttcga
     2041 ccagtgccgc gatgctggga gctgccgaca agcgcagtcg cgccggtgtg tccatgaagc
     2101 tgacgtgcca cggctcgcct cgatcgatcg cggccagatc gacgaccacc ggcaacggcg
     2161 tggtcgcgtc agcgacggtt tgccagccga ccgcgtgcac acaacccggt ggcgcgatcg
     2221 acccgtggaa acgttgcacg caacgatcga gccaacgcgc tgtcggcgtt ggaggcgacg
     2281 atgccgcggt cgacgtcgag ccttcggcga ccatctgtgc gccgagccac cccagggcgg
     2341 gagccgcgtc gacaccgaag acaacgaccg actcgctgac cggccccagc cgcaacaggt
     2401 cggcctcccc cggggccagg ccatcattgg cgatgccgtc ggacgtgcgc gcccaacacg
     2461 tgatggccac ctcgtcggtc gtgaggccag caaggtcacc catcacggcc accatccc
//
LOCUS       DAORNF010000518         2517 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_31265_length_2517_cov_1.638505, whole genome shotgun sequence.
ACCESSION   DAORNF010000518 DAORNF010000000
VERSION     DAORNF010000518.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2517
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31265_length_2517_cov_1.638505"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..586
                     /locus_tag="PLV68_17100"
     CDS             <1..586
                     /locus_tag="PLV68_17100"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HRE03019.1"
                     /translation="FDAAVAAVADAGGEVIELDLPGAEHGATLSWLITMYEAAQTYRD
                     APREQLTAAFVSRLDVGDRITADEYHRALVARSYLAAEARTVFAARGAADTAIDVVMV
                     PSTVSTAPPADDVDRPVVGVPATWPDVLARTIAYFNVTGLPAVSVPAGHGVGGAPVGV
                     QFAGAPFADERVLHIASWFHDALQPATSAQTERT"
     gene            590..1282
                     /locus_tag="PLV68_17105"
     CDS             590..1282
                     /locus_tag="PLV68_17105"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HRE03020.1"
                     /translation="MHHVYDFSAHGAGTNQGLLSQLVVPRPIAMVSTRDVHGAGNLSP
                     FSYYMPVTGSPPLVTIAFGARESDGDTKGSFQNLMASGDFVINVCSEVFADHIEGLAK
                     EYPHGVDEAAVHGFTWRDSQRVSSPAVAEASAALECIVHTTFDLGDETAPVTLVVGEV
                     LCAVIDDSVLASSDLDHPRIDLLALAPIGRSGARTFIRAIEQAVYHQERHPYVPEPDQ
                     QQRDKNSGVLRT"
     gene            1279..2313
                     /locus_tag="PLV68_17110"
     CDS             1279..2313
                     /locus_tag="PLV68_17110"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HRE03021.1"
                     /translation="MKALQYDHHGGPEVLTYVDVAEPYTSPADVLVEVAATALNHLDI
                     VQRNGWFTMPGYSLPHIAGMDVVGTVIAAGDEVDGVRVGDRVVVDPSLAGVSDNSRLA
                     GMGDLYGELGVIGGTVAGGYAERCLAPGSHVHRIPDSMSWEHAVVFPSAWMTAHHAIF
                     AVGHLQFGETILIHAAGSGVSSAAIQLAKHAGATVLATAGSEDKCERALALGADHVAN
                     NRDTDITAWARSVTEGAGVDIVFDHVGPALWAQSMFALKPRGRLVNCGNTTGDTATIP
                     SLGFMFHMGIQILGSDPYRYEEFGSAWHTYCHGGFESAIDSVFPLAEGAAAQQKMERS
                     DFFGKILLKP"
BASE COUNT          376 a          876 c          862 g          403 t
ORIGIN      
        1 gttcgatgct gctgtcgctg cggtggccga cgcgggtggc gaagtgatcg agctcgacct
       61 gccgggagcc gaacacgggg ccacgctgag ctggctgatc acgatgtacg aggccgcgca
      121 gacctatcgc gacgccccgc gtgagcagtt gaccgcggcg ttcgtctcgc ggctcgacgt
      181 cggcgatcgg atcaccgccg acgagtacca ccgcgccctc gtcgcccggt cgtacctcgc
      241 cgccgaggcg cgcaccgtgt tcgccgcacg aggtgccgcc gacaccgcga tcgacgtcgt
      301 gatggtgccg tccacggtca gtaccgcccc acccgccgac gacgtcgacc gcccggtcgt
      361 gggcgttccc gccacctggc ccgatgtgct ggcgcgcacg atcgcctact tcaacgtcac
      421 cgggctccct gctgtgtcgg tcccggccgg ccacggcgtc ggcggcgcac ccgtcggcgt
      481 gcagttcgca ggcgcgccgt tcgccgacga gcgggtgctc cacatcgcgt cgtggttcca
      541 cgacgctctc caacccgcca cctccgccca gaccgaaagg acctgaccgg tgcatcacgt
      601 ctacgacttc tccgcgcacg gcgcgggcac caaccagggg ctgctgtccc aactggtggt
      661 gccccgcccg atcgcgatgg tgtccacccg cgatgtgcat ggcgcgggca acctgtcgcc
      721 gttcagctat tacatgccgg tcaccggctc gccgccactc gtcacgattg ccttcggtgc
      781 ccgcgagtcc gatggggaca ccaagggttc gttccagaac ctgatggcca gcggtgactt
      841 cgtgatcaac gtctgctccg aggtgttcgc cgaccacatc gaaggcttgg ccaaggagta
      901 cccgcacggg gtcgacgagg cggccgtgca cggcttcacc tggcgcgact cgcaacgggt
      961 cagctcgccg gcggtcgccg aagcgtcggc cgcgctggaa tgcatcgtgc acaccacgtt
     1021 cgatctcggt gacgagaccg cgccggtgac gctggtggtg ggcgaggtgc tgtgcgcggt
     1081 gatcgacgac tcggtgttgg cctcgtccga tctcgaccat ccgcgtatcg acctgctcgc
     1141 cctggccccg atcggtcgct cgggagcgcg gacgttcatc cgcgccatcg agcaggccgt
     1201 gtaccaccag gaacgtcacc cgtacgtgcc agagccagac cagcaacagc gagacaagaa
     1261 cagcggggtg ctccggacat gaaggcattg cagtacgacc accacggcgg gccggaggtg
     1321 ctcacctacg tcgacgtcgc cgagccgtac acgagcccgg ccgacgtgct ggtcgaggtt
     1381 gccgccaccg cgctcaacca cctcgacatc gtgcagcgca acggctggtt cacgatgccc
     1441 ggctactcgc tgccgcacat cgccgggatg gacgtggtgg gcaccgtgat cgccgctggt
     1501 gacgaggtcg acggggtgcg cgtcggcgac cgggtcgtgg tcgacccctc gctggccggg
     1561 gtcagcgaca actcccgcct ggcagggatg ggcgacctgt acggcgaact gggcgtcatc
     1621 ggcggcaccg tcgccggggg gtacgccgag cgctgcctcg cccccggcag ccacgtccat
     1681 cgcatccccg actcgatgag ttgggagcac gcggtggtgt tcccatcggc gtggatgacg
     1741 gcccaccacg cgatcttcgc ggtgggccac ctgcagttcg gtgagacgat tctgatccac
     1801 gctgccggca gcggcgtgtc gagcgcggcg atccaactgg ccaagcacgc gggcgcgacg
     1861 gtgctcgcca ccgcgggcag cgaggacaag tgcgagcggg cgctcgcgct cggtgccgat
     1921 cacgtcgcca acaaccgcga caccgacatc accgcgtggg cgcgcagcgt caccgagggc
     1981 gctggggtcg acatcgtgtt cgaccacgtc ggcccggcgc tgtgggcgca gtcgatgttc
     2041 gccctgaagc cgcgcggtcg gttggtcaac tgtggcaaca ccacgggtga cacggccacc
     2101 atcccgtcgc tggggttcat gttccacatg ggcatccaga tcctcggctc cgacccgtac
     2161 cgctacgagg agttcggctc ggcctggcac acgtattgcc acggtgggtt cgagtcggcg
     2221 atcgacagcg tgttcccgtt ggccgaaggc gccgcggccc agcagaagat ggagcgctcc
     2281 gacttcttcg gcaagatcct gctcaagccg tgagtgggcc ggttctgacg atgcgttggt
     2341 gttggatgcg ttggtgtgga tggtcgattc gccgtatccc gagctgaaga agacgcacac
     2401 gatggcccgt cgcggccgcc cgtggcgcga ctacaagccg ccgcccgcgg cgccggtgtg
     2461 ggcagtgatc cagggctacg gctcgtattg gacgctggtc gccgcggtcg acctggg
//
LOCUS       DAORNF010000519         2515 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_31306_length_2515_cov_1.483740, whole genome shotgun sequence.
ACCESSION   DAORNF010000519 DAORNF010000000
VERSION     DAORNF010000519.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2515
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31306_length_2515_cov_1.483740"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    302..370
                     /estimated_length=69
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<371..1299)
                     /locus_tag="PLV68_17115"
     CDS             complement(<371..1299)
                     /locus_tag="PLV68_17115"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HRE03022.1"
                     /translation="MLDVLGPDGWYVNYSVVHAGGVSYKAGGEADAISARSPDGSCVI
                     VASERDHHSIATYALDGHEVWRISGTLGCADSVTGTVLGWSASGRLMGWSITDGSVIW
                     ESGITSSETDALDGKCASGVVAFATDGGGPAFTVDARTGSRSRTAQLPGGDLDGFGSD
                     FPVALSSDVPYLAGLSSGESPIAWERNDIRTGSRIVVVDGTFKLAAAVVGADEDHSYL
                     VLIDAATGREEWVFDLDSDEEYGCGTCFSDIDQSGPTTLIVRGEFGIDLRTGQQLFRN
                     ENDSCGNSSIGWPQCNQDGITTYLDPTTGGVVWS"
     gene            complement(1709..>2186)
                     /locus_tag="PLV68_17120"
     CDS             complement(1709..>2186)
                     /locus_tag="PLV68_17120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03023.1"
                     /translation="LPDGTDTAVITFHNGADPITLTGLGAGDHEHHVDLTLPAGPLLA
                     LIGTVTVGGQAPFTPDEQVWVTIEAYSVVEGLPPQIEGEGGETGPGGWLRLATLYPEL
                     DYSNPDGSMQLTVALPPGTTHANLFLEARTASGWSSHEQTLPITEHQPPTSLTVTL"
     assembly_gap    2187..2309
                     /estimated_length=123
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          409 a          748 c          735 g          431 t
ORIGIN      
        1 gagtcaactg gctcgtacag catcgaatat tcgctggcat cgcccagcac gagtgctctc
       61 acgcactcca tgtaccccac agactcgatc cccgcgcctt ggccgatgta cgggacacag
      121 tcctgagcgg cgcggttcag cagagcgagt gcagcatcgc tgtccgatgc ttccgtatca
      181 cgtgcctggt cattctcatc gtgcgagtag tcgtatcaca ggtttccgaa ttcgacgacc
      241 aaccccgggc cgacttgcca caggatctcg ccggtcgccg cggccagacc ggcgatggtg
      301 tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn gaccacacca cgcctccggt cgtcgggtca aggtaggtgg tgatgccgtc
      421 ttggttgcac tgcggccagc cgatcgagga gtttccacac gagtcgttct cgttccggaa
      481 gagttgctgg ccggtgcgca gatcgatacc gaactcacct ctaacgatca atgttgtcgg
      541 tccgctctgg tcaatgtccg aaaaacaggt accacatccg tactcctcgt cggagtcgag
      601 atcgaacacc cattcttcgc gaccggtcgc agcgtcgatc agcacgaggt acgagtggtc
      661 ctcgtccgca ccgacgactg ccgcagccag cttgaaggtg ccatcgacga cgacgatcct
      721 ggacccggtg cggatgtcgt tccgctccca ggcgatcggt gactcgcccg acgacaatcc
      781 tgcgagatac ggaacgtcac tgctcaaggc aaccggaaaa tctgatccaa acccgtctag
      841 atcgcccccc ggcaactgtg cggttcggct acgcgagcct gtgcgtgcat ccaccgtgaa
      901 ggcgggacca ccgccgtcgg tggcaaacgc gacgacacct gaggcacact tcccgtcgag
      961 tgcatcagtt tcggagctcg tgatgccgga ctcccagata acgcttccat cagtgatcga
     1021 ccagcccatc agtcggcccg aggcggacca ccccaacact gtgcccgtga ctgaatcagc
     1081 gcatccgagc gtgccggaga tacgccagac ctcgtgcccg tcgagggcat atgtcgcgat
     1141 cgagtgatga tcgcgctccg atgcaacgat cacacacgag ccgtcgggtg accgagccga
     1201 aatcgcgtcc gcttcgcccc ccgccttgta gctgacccct ccggcatgta cgaccgagta
     1261 gttgacatac caaccgtctg gcccgagcac gtcgagcatc tcgacgtcac cttccgttcg
     1321 cgagtggatc tcccagagca gttcgccgcc cgacaggtca tacgcgctga tccccgatcc
     1381 gtcgtcggta agaccgagga cggattcttg cggactcacg aagcgcgggt gtgtgctggc
     1441 gtaacagggt gtgtccgagg cggcctcgct cgtcggtacg gtggtctgca acgtcggggc
     1501 gggcgctccg gcggctgacg ccactgtgtt cgcatccggt gtcgttggta cggtcaccga
     1561 tgaggtcgac cgccctacgg cggtcgagtc gcggttaccg ctggtggtcg cagcggaggc
     1621 acacgccgac agcaccaaca ccagccccgc tgatagcgac tggggcagtc gtttcatgcg
     1681 tctctcccgt ttatcgcagg gattgtagct acagggtgac ggtcagggag gtggggggtt
     1741 ggtgctcggt gatggggagg gtttgctcgt gggaggacca gccggaggcg gtgcgggctt
     1801 ccaggaagag gttggcgtgg gtggtgccgg gcgggagggc gaccgtgagt tgcatcgagc
     1861 cgtcggggtt ggagtagtcg agctcgggat acagcgttgc cagccgcagc caacccccag
     1921 gaccggtctc gccaccttcg ccttcgatct gcggaggcaa accctcgacc acgctgtacg
     1981 cctcgatcgt cacccacacc tgctcgtccg gcgtgaacgg cgcctgaccg ccgacggtga
     2041 cggtgccgat cagcgccagc agcggcccgg cgggcagagt caggtcgacg tggtgctcgt
     2101 ggtcgcctgc gccgagcccg gtcaacgtga tcggatcagc cccgttgtgg aacgtgatca
     2161 ccgccgtgtc ggtgccgtcg ggcaacnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc gctgccgtca tcgatggtgg ccgtaccgct
     2341 gacctctaac caggtcagct cgacgtcgtg atgcgacgtg ctgagcaccc cgggagtgat
     2401 cgccatctcg tgcgttgtgc cgagccatgt cggatatccc gtgacttgcg cgattgttgt
     2461 tccgaccgga acatcgaacg cgaccgcgta ctcaccgggc gtcatctggt cgaag
//
LOCUS       DAORNF010000520         2512 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_31352_length_2512_cov_1.728124, whole genome shotgun sequence.
ACCESSION   DAORNF010000520 DAORNF010000000
VERSION     DAORNF010000520.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2512
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31352_length_2512_cov_1.728124"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="PLV68_17125"
     CDS             complement(<1..320)
                     /locus_tag="PLV68_17125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03024.1"
                     /translation="MESLDLAELAARATRAAQGWSPGATVHDITVLHGGQSSLTFSAL
                     AGDRQIALKVAPPGLAPVRNRDVLRQARAITALGTVPGMKVPAVLFADGGDPPDVPPL
                     FAMS"
     assembly_gap    329..341
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<342..1161)
                     /locus_tag="PLV68_17130"
     CDS             complement(<342..1161)
                     /locus_tag="PLV68_17130"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRE03025.1"
                     /translation="MKMQFSGRLAIVTGASRGIGEAIAHRLARGGATVVITARTMQPA
                     EGAVGSLAEVAAAINAASAVNGGRAIPIQADLSKPADREHLIAETSEHGPVDILVNNA
                     AVAFLAKGSEYPERRTRLMFEVNVHAPMDLCQRVVPAMKERGAGWIVNISSVGAVHPL
                     GPPYELLHSRGIYTVYGMCKAALDRLSTGLAAELNEYGIAVNSLSPWGWVPTPGTMTN
                     DIDGLGDASSVEDSSVIAEAAAFLCSADGRRVSGRVAYSQPLLNEFQLSPVNDNR"
     gene            1281..>1506
                     /locus_tag="PLV68_17135"
     CDS             1281..>1506
                     /locus_tag="PLV68_17135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HRE03026.1"
                     /translation="MAVGIVAYGAYVPHHRLNRASIDAALGNNGGRGHRSVANYDEDT
                     TTMAVEAARIARRRLGPAAIDGVYFATATPA"
     assembly_gap    1507..1686
                     /estimated_length=180
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1687..>1924
                     /locus_tag="PLV68_17140"
     CDS             <1687..>1924
                     /locus_tag="PLV68_17140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HRE03027.1"
                     /translation="SDERDGGDAGAAVIVGSGPGVVAEIVSFASVTEEFLDRWRLPEE
                     RSSHVWEERFGEQVVVPLAQQATGEACRAAGITPS"
     assembly_gap    1925..1950
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1951..>2512
                     /locus_tag="PLV68_17145"
     CDS             <1951..>2512
                     /locus_tag="PLV68_17145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HRE03028.1"
                     /translation="AGLHERAVKSLPKALGLTGVLAPNLTDVIGNSGTAHGALVLTDV
                     LDRAEPGQHILVVSVADGADAIVLRTTAAITDYRAATAAHYTTHQVAAQVARGDLDLP
                     YAMFLTWRGYLHREPPRREEPDRPMSPVMARRDSWKFGLVGSTCTACGTRNMSPQRVC
                     FSCQAVDEMEAECFADVPATITTFTID"
BASE COUNT          356 a          768 c          788 g          381 t
ORIGIN      
        1 aaggacatcg cgaagagcgg cggcacgtcc ggtgggtcac cgccgtcggc gaacagcacg
       61 gcagggacct tcatcccggg caccgtgccg agtgccgtga tcgcccgggc ctggcggagc
      121 acgtctcgat tgcggactgg tgccagcccg ggcggggcga ccttgagcgc gatctgccgg
      181 tcgcccgcaa gtgccgagaa ggtcaggctc gactgcccgc cgtgcagcac ggtgatgtcg
      241 tggacggttg ctccaggcga ccagccctgc gcggcgcggg tcgcccgcgc ggccagttcg
      301 gcgaggtcga gcgactccat cgccccagnn nnnnnnnnnn ngccggttgt cgttgacggg
      361 cgagagctgg aactcgttga gcaacggctg gctgtaggcc acacgtccgc tgacccggcg
      421 accatcggcc gaacacagga aggcggcggc ttccgcaatc accgacgagt cctcgacgga
      481 ggatgcgtca ccgagtccgt cgatgtcgtt ggtcatcgtg cccggagttg gcacccaacc
      541 ccatggtgag agcgagttga cggcgatgcc gtactcgttg agttcagcgg caagcccggt
      601 cgagagacgg tccaacgcgg ccttgcacat gccgtacacg gtgtagatcc cgcgactgtg
      661 gagcagttcg tacggaggcc ccagaggatg cacggcgccg acggaggaga tgttcacgat
      721 ccagccggcg ccgcgctcct tcatcgccgg gacgacccgc tggcagaggt ccatgggcgc
      781 gtggacgttg acctcgaaca tcagccgcgt tcgccgttcc gggtactcgc tgcccttcgc
      841 cagaaacgcg accgcggcgt tgttgacgag gatgtccacc gggccgtgct cgctggtctc
      901 ggcgatgagg tgctcccggt cggctggctt ggagaggtcg gcctggatcg ggatcgcccg
      961 gccgccgttc accgccgatg cagcattgat ggccgcggcg acctcggcca gcgagccgac
     1021 cgctccctcg gccggttgca tcgtccgcgc ggtgatcacg acggttgcgc cgccccgcgc
     1081 gagacggtgt gcgattgctt cgccgatgcc gcggctcgct cccgtgacga ttgccaagcg
     1141 acccgagaac tgcatcttca tcacgtaccc ctccgcgtcc aggacgcagt ctagcccgag
     1201 ggaaagttga ctggttggtt ggtgcgaggt taggttgccc tctgttctgg agagacggtt
     1261 ccggagggag acgcgcaatc atggctgtcg ggattgtcgc ctacggcgcg tacgtgccgc
     1321 atcaccgtct caaccgagca tcgatcgacg cggccctggg caacaacggt gggcgcggtc
     1381 atcgctccgt ggccaactac gacgaggaca cgaccacgat ggccgtcgag gcggcccgga
     1441 tcgcacgcag acgtctcggt ccggctgcga tcgacggtgt ctacttcgcc accgccaccc
     1501 cggcctnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnncagt gacgagcgcg acggtggcga tgccggtgct gccgtgatcg tcggttcggg
     1741 tcccggagtc gtcgccgaga tcgtcagctt tgcttcggtg accgaggagt tcctcgaccg
     1801 ttggcgcctg cccgaggagc gatcgtcgca cgtgtgggag gaacgcttcg gcgagcaggt
     1861 cgtcgtgccg cttgcgcagc aggcgaccgg agaagcgtgc cgcgccgctg gcatcacccc
     1921 cagcnnnnnn nnnnnnnnnn nnnnnnnnnn gccggtctgc acgaacgtgc cgtcaagagc
     1981 ctgccgaagg cgctcggcct cacgggtgtg ctcgccccca atctgaccga tgtgatcggc
     2041 aacagcggta ccgcgcatgg cgcgctggtg ctgaccgacg tgctcgaccg tgccgagcct
     2101 ggtcagcaca tcctcgtcgt cagcgttgcc gacggtgctg acgccatcgt cttgcgcacc
     2161 acggcggcga tcaccgacta tcgggcggcg accgcggccc attacaccac tcatcaggtc
     2221 gcggctcagg tagcgcgtgg cgacctcgat ctgccgtatg ccatgtttct cacctggcgc
     2281 gggtatctgc accgcgaacc gccgcggcgt gaagaaccgg accgcccgat gtcaccggtg
     2341 atggcacgcc gcgactcgtg gaagttcggc ctcgtcggct cgacgtgcac ggcctgcggc
     2401 acgcgcaaca tgtctccaca gcgggtctgc ttcagctgtc aggctgtcga cgagatggaa
     2461 gccgagtgct tcgcggatgt cccggcgacg atcaccacgt tcacgatcga cc
//
LOCUS       DAORNF010000521         2512 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_31354_length_2512_cov_1.613350, whole genome shotgun sequence.
ACCESSION   DAORNF010000521 DAORNF010000000
VERSION     DAORNF010000521.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2512
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31354_length_2512_cov_1.613350"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..714
                     /locus_tag="PLV68_17150"
     CDS             <1..714
                     /locus_tag="PLV68_17150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03029.1"
                     /translation="VRDLGPSVRIEARHAALAADHAALIDAFTRLGEASGQARWITHA
                     IDVADMTLDHFWDHDHGGVFTTADDGEALIVRHKDLFDQATPSANSTLAIALYRLSAL
                     TDEQRFANHADRIVQLLATPAAQNPAAFGNAMAAIDLRITGTTEIVITGDRPDLVATA
                     RRILLPNAVFAWGERYDSPLWADRPDGFAYVCRNFTCSHPASTPQAMIEAIIGRPLDA
                     TNPQSGSGPASHDDPTPEN"
     gene            complement(718..1308)
                     /locus_tag="PLV68_17155"
     CDS             complement(718..1308)
                     /locus_tag="PLV68_17155"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HRE03030.1"
                     /translation="MVRSRRMTRSFRDAPIEPELIDRLVDLGSRSPSAGKTQGWHVVV
                     LEGAATARLWDITLPSPRRESFRWQGLLRAPVIMLPLADPDAYVARYAEVDKQASGLG
                     RGTEAWPVPYWTIDASMAMMTMLLAAQDAGLGALLFGIFRGEDELRASLGIPANLELL
                     GAIALGWPDTAAADDTGLSASRPRRAPGAIIHRNGW"
     gene            complement(1344..2057)
                     /locus_tag="PLV68_17160"
     CDS             complement(1344..2057)
                     /locus_tag="PLV68_17160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03031.1"
                     /translation="MAVQVDTRDSAALSDTDLDEMASIGGSFDIGTVSKAKEDWVLST
                     AARIDGTLHGYMFATLERIGGTPCVLIGLISVKRTSKRDQALKGLIGMLSVKRSSKRD
                     QVLKGLMGEAFHRALMAFPDEDVVVGTRMVSADGFEAFKQIGDVIPRPGHRAVGEERA
                     WGRRLAKRFAVDANYDEQSFVVKANGHSGYLDHESSKPEKLSADVVAQFTAVNRKKGG
                     VLIVHGWTMAEDLVKLGKR"
     gene            complement(2191..>2512)
                     /locus_tag="PLV68_17165"
     CDS             complement(2191..>2512)
                     /locus_tag="PLV68_17165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03032.1"
                     /translation="AIVTVKGRLDRKDETRVGFMVQDVQVLEGLDTSKSQPLRLRLPA
                     TGLNEIKIQQLKRILRDHPGESQVFVHLGGGKILRLADEFCVDLDRAVGELRVAFGHD
                     AVIL"
BASE COUNT          431 a          902 c          785 g          394 t
ORIGIN      
        1 gtgcgcgacc tcggcccgag cgtgcgcatc gaggcgcgcc acgccgcgct cgctgccgac
       61 cacgcggccc tgatcgatgc gttcaccagg ctgggcgaag cgagcggcca agcccgctgg
      121 atcacccacg cgatcgacgt tgccgacatg accctcgatc acttctggga ccacgaccac
      181 ggcggtgtgt tcaccaccgc cgacgacggc gaggcgctga tcgtgcgcca caaggatttg
      241 ttcgaccagg ccacgccgtc ggccaactcg accttggcga tcgcgctgta ccgcctgtct
      301 gcgctcaccg acgagcaacg cttcgccaac catgccgatc gcatcgtgca gctgttggcc
      361 accccggcag cccagaaccc ggcggcattc ggcaacgcga tggcggcgat cgatctgcgc
      421 atcaccggca cgacggagat cgtcatcacc ggcgaccgcc ccgacctggt cgcgacggcg
      481 cggcgcatcc tgctccccaa cgcggtgttc gcctggggcg agcgttacga ctcgccattg
      541 tgggccgacc gacccgacgg cttcgcctac gtctgtcgca acttcacctg ctcccacccc
      601 gccagcactc cccaagccat gatcgaagcc atcatcggac ggcctctcga cgccacgaac
      661 cctcagtcgg gcagcggccc agcgtcccac gacgacccaa cgccggagaa ctgaacctca
      721 ccagccgttg cggtggatga tcgcgccggg cgcacgccgc ggccgtgacg cgctgaggcc
      781 cgtatcgtcg gcagcggcgg tatctggcca acccagggcg atggcgccga gcaactccaa
      841 gttggcgggg atgccgaggc ttgctcgcaa ctcgtcctcg ccacggaaga tgccgaacag
      901 cagcgccccc aggccggcgt cttgggccgc gagcagcatc gtcatcatcg ccatcgaggc
      961 gtcgatggtc caatacggca ccggccaggc ctcggtacca cgacccagtc cactcgcctg
     1021 cttgtccacc tcggcgtaac gggccacgta cgcgtcgggg tcggccaggg gcagcatgat
     1081 caccggggcg cgcaacaggc cctgccaccg gaacgactcc cgtcgggggg acggcagcgt
     1141 gatgtcccac agccgggccg tcgccgcgcc ttccagcacc accacgtgcc aaccctgggt
     1201 cttgcccgcg ctcgggctgc gtgagccgag atcgacgaga cgatcgatca gctccggctc
     1261 gatcggcgcg tcgcgaaacg agcgcgtcat ccgccgcgag cggaccagcg cgtcgaacga
     1321 cgcggcgggc gggccgagac cgctcagcgc ttaccgagct tgacgaggtc ttcggccatg
     1381 gtccacccgt ggacgatcaa cacgccgccc ttcttgcggt tgaccgcggt gaactgagcg
     1441 acgacgtcgg ctgagagctt ctccggcttg gacgactcgt ggtcgaggta cccgctgtgg
     1501 ccgttggcct tgacgacgaa gctctgctcg tcgtagttgg catcgacggc gaaccgcttg
     1561 gcgagacggc ggccccaggc ccgctcttcc ccgaccgcgc ggtgaccggg gcgagggatg
     1621 acgtcgccga tctgcttgaa cgcctcgaac ccgtcggccg acaccatgcg ggtgccgacc
     1681 acgacgtctt cgtcgggaaa ggccatgagc gcgcggtgga acgcttcgcc catcagaccc
     1741 ttgagcacct ggtcacgctt ggacgaacgt ttcacgctga gcatgccgat cagccccttg
     1801 agggcctggt cacgcttgga ggtgcgcttc acgctgatca ggccgatcag cacgcaggga
     1861 gtgccgccga tgcgttcgag ggtggcgaac atgtacccgt gcagggtgcc atcgatgcgc
     1921 gccgccgtgc tgagcaccca gtcctctttg gccttcgaca ccgtgccgat gtcaaagctg
     1981 ccaccgatcg aagccatctc gtcgagatcg gtatcgctga gcgccgctga atccctggtg
     2041 tcgacctgga ctgccatcga atctctgcgc tccctcacgt cgcggtaccg ctgtaccttg
     2101 ggcaatgaag tacccattct gccacgattt cccaccgatc agcccgaaaa ccgcgctgac
     2161 gacgtaaaac gcgggcaccc tgggtggcgt ctacaagatc acggcgtcgt gaccgaaagc
     2221 gacccgcaac tcgccgacag cgcggtcgag atcgacgcag aactcgtcgg ccaagcgcaa
     2281 gatcttgccc ccaccgaggt ggacgaacac ctgcgattca cccgggtgat cacgcagaat
     2341 ccgcttgagt tgctggatct tgatctcgtt cagcccggtg gccggaagcc gcagacgcag
     2401 cggctgcgac ttgctggtgt caaggccttc cagcacctgc acgtcctgca ccatgaaccc
     2461 gacgcgcgtc tcgtccttgc ggtcgagacg gcccttgacg gtgacgatcg cg
//
LOCUS       DAORNF010000522         2511 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_31370_length_2511_cov_1.688925, whole genome shotgun sequence.
ACCESSION   DAORNF010000522 DAORNF010000000
VERSION     DAORNF010000522.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31370_length_2511_cov_1.688925"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="PLV68_17170"
     CDS             <1..258
                     /locus_tag="PLV68_17170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03033.1"
                     /translation="VEGARWLALEAAESGSAEGAAYALAHAVEAVKLVFPMTHQYSGA
                     IGFTQEYDLHLATMRTVAIRAEGELFGRPATAAACEHWSFP"
     gene            complement(296..880)
                     /locus_tag="PLV68_17175"
     CDS             complement(296..880)
                     /locus_tag="PLV68_17175"
                     /inference="COORDINATES: protein motif:HMM:NF014664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HRE03034.1"
                     /translation="MPHLDPVDADTDDPVLRNVFDVFVDSGRDVPMLYRLLGNSPDML
                     AAWVGLAWPLRAQPTTSRALRELIIMRIAVLTQASYEWSAHWPAALKAGVTTPQLRAL
                     GIGAASDEFDAAQRATLRCTDEIILDGGASASAMAELRMHFSDGECVELVLTATFYAC
                     VSRTLASLGLEADAPPDEPSRAMFDAIVESMPSR"
     gene            955..1668
                     /locus_tag="PLV68_17180"
     CDS             955..1668
                     /locus_tag="PLV68_17180"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRE03035.1"
                     /translation="MTLDGRTVMIVGGTSGLGLATARRLKDAGAVLAIVGRDAAKVAD
                     ARRLLGPDVTVESVDAADASALHAFVAGLDKIDHVISFAGLQPAAPIGQADHAMFASA
                     FDARVWAARNCCAAAIDRMPPHGSFTFCSGLSAVRPRAGRSAGAVATAVESFARAMAV
                     ELAPIRVNAICPGAFDTEVLRRAFGADHEAALARIARSVPLGRVGQPDEIAHAIEFLI
                     CNTYTTGITLRVDGGALLV"
     gene            1679..>2241
                     /locus_tag="PLV68_17185"
     CDS             1679..>2241
                     /locus_tag="PLV68_17185"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRE03036.1"
                     /translation="MSDLLQGIRVLESAVLLNGDTLGMHLADLGADVIKIESPGSGDY
                     IRDILGQIAPRNSPAHLQVNKNKRSVAIDLRTDAGRDVFWDLLATADVFVDGFAGAAT
                     DRLGIGYEQQRLCKPNIVYCQYTGFGRAGPYAAIPTHGQMMNAEAAAVTLVTHADGFV
                     RIAENRELMWGTSIGGDGTAAGAVHAAL"
     assembly_gap    2242..2267
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2268..>2511
                     /locus_tag="PLV68_17190"
     CDS             <2268..>2511
                     /locus_tag="PLV68_17190"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRE03037.1"
                     /translation="VRRDRTGQGAWLDASAADAVIAQGWIGAVYGLNYDRITDHTGLR
                     TPGSESLTGAKYQYYATLDDRYVLFCCIEPKFWTRFC"
BASE COUNT          393 a          814 c          853 g          425 t
ORIGIN      
        1 gtcgaggggg cgcgttggct cgcgctcgaa gccgccgagt cgggcagtgc ggagggcgcc
       61 gcgtacgcgc tcgctcacgc cgtcgaggca gtcaagctcg tcttcccgat gacccaccag
      121 tactcgggtg cgatcggttt cacgcaggag tacgacctcc acctggcgac gatgaggacc
      181 gtggcgatcc gcgccgaagg cgagttgttc ggacgtccgg cgacagccgc ggcgtgcgag
      241 cactggtcgt tcccgtagac cggcccccca catcgaaggc tcggcgcagc gggactcagc
      301 ggctcggcat cgactccacg atcgcgtcga acatggcgcg cgacggctcg tctggcgggg
      361 cgtcggcttc gagacccagc gacgccaacg tccgcgagac gcaggcgtag aaggtggccg
      421 tcagcaccag ttcgacgcac tcgccgtcgc tgaagtgcat ccgcagttcg gccatggcgg
      481 acgccgaagc acccccgtcg aggatgatct cgtcggtgca gcgcagggtc gcgcgttggg
      541 cggcatcgaa ctcgtcggat gctgcaccga tgccgagggc gcggagttgg ggtgtcgtca
      601 ctcccgcctt cagcgcggcg ggccagtgag cgctccattc gtagctcgcc tgagtgagga
      661 cggcgatgcg catgatgatc agctcgcgca gggcccggct ggtcgtgggc tgggcccgca
      721 gcggccacgc caatccgacc caggccgcga gcatgtcggg cgagttgccc agcaggcggt
      781 acaacatcgg tacgtcgcgg cccgaatcga cgaacacgtc gaagacgttt cgcagcacgg
      841 gatcgtcggt gtcggcgtcg accggatcga ggtgtggcat cgtaggtcct ctcggcgagg
      901 ctgtcatctt gacgggctcc acaattcagc atactctatt atccggaggt atcgatgacg
      961 ctcgacgggc gcacggtgat gatcgtcggt ggcacgtcgg ggttgggact ggcgacggcg
     1021 cgccgcttga aggatgcggg ggccgtcttg gcgatcgtgg ggcgagacgc ggccaaggtg
     1081 gccgacgcgc gtcgattgct cggccccgat gtcacggtcg aatcggtcga cgcggccgat
     1141 gcatcggcgc tgcacgcgtt tgtggccggc ctcgacaaga tcgatcacgt gatctccttt
     1201 gccggcctgc aaccggcggc accgatcggc caagcagacc atgcgatgtt cgcgtcggcc
     1261 ttcgatgccc gtgtctgggc ggcgagaaac tgctgcgccg cggcgatcga tcgaatgcct
     1321 ccccacggat cgttcacgtt ctgctctggg ttgtctgccg tgcggcctcg cgccggcaga
     1381 tctgctggtg ccgtcgcgac cgcggtggag agcttcgcca gggcgatggc cgtcgaactg
     1441 gccccgattc gcgtcaatgc gatctgcccg ggggcgttcg ataccgaagt gctccgccgc
     1501 gccttcggcg cagaccacga agccgccctt gcccggatcg cccggtcggt gccgctcgga
     1561 cgagtcggcc agcccgatga gattgcccac gcgatcgagt ttctgatctg caacacctac
     1621 accaccggca tcacgttgcg tgtcgacggt ggagccctgc tcgtgtgatg cgcgtccgat
     1681 gagcgacctg ctgcagggca ttcgtgtgct cgagtccgcc gtgctgttga acggcgacac
     1741 actcgggatg caccttgccg atctcggggc cgacgtgatc aagatcgaat ctccggggtc
     1801 tggcgactac atccgcgaca tcctcggtca gatcgcgccg cgcaacagcc cggcccatct
     1861 ccaggtcaac aagaacaagc gcagcgtcgc gatcgatctg cgcaccgacg ccggccgcga
     1921 tgtgttctgg gatctcctcg ccactgccga cgtgttcgtc gacggattcg cgggtgcggc
     1981 gaccgatcgg ctcggtatcg gctacgagca gcagcgcctc tgcaagccaa acatcgtgta
     2041 ctgccaatac accggtttcg gccgcgccgg cccgtatgcg gcgatcccaa cgcatggaca
     2101 gatgatgaat gccgaggccg ccgcggtcac gctcgtcact catgcggacg ggttcgtgcg
     2161 gatcgccgag aaccgcgagc tgatgtgggg cacctcgatc ggcggtgacg gcacggccgc
     2221 tggtgccgtg cacgccgcgc tnnnnnnnnn nnnnnnnnnn nnnnnnngtt cgccgcgatc
     2281 gcacggggca gggggcgtgg ctcgatgcgt ccgcagccga cgccgtcatc gcgcagggct
     2341 ggatcggggc cgtatacgga ctgaactacg accgcatcac cgatcacacc ggattgcgta
     2401 cgcccgggag tgaatcgctg accggcgcca agtatcagta ctacgccacg ctcgacgatc
     2461 gctacgtgct gttctgttgc atcgagccga agttctggac gcgcttctgc c
//
LOCUS       DAORNF010000523         2508 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_31424_length_2508_cov_1.538932, whole genome shotgun sequence.
ACCESSION   DAORNF010000523 DAORNF010000000
VERSION     DAORNF010000523.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2508
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31424_length_2508_cov_1.538932"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>232)
                     /locus_tag="PLV68_17195"
     CDS             complement(<1..>232)
                     /locus_tag="PLV68_17195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03038.1"
                     /translation="TGAPQISNNWGDLVTMLDACLVNGFALKAIDTLTFADGIATATI
                     SSGHAYRPFQVVEIAGAEQPEYNGSFRVLTTTM"
     assembly_gap    233..409
                     /estimated_length=177
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<410..>1364)
                     /locus_tag="PLV68_17200"
     CDS             complement(<410..>1364)
                     /locus_tag="PLV68_17200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03039.1"
                     /translation="KIEATGINGSVDYETGLVRVRFGTTVTAAGNESEPWYAADRVGT
                     DGKIFRPEPVAASSVRYSAVAYSYLPLDADLLGIDPVRLPSDGRVPIFRPGGFAVVGH
                     TGKITTSVSNGQTIDCARVRLSRVRVVGHDGSVINTGYTADLEAGTVTFTNVTGFSQP
                     VTVEHRIEDMAVVRDVQINGEISFTRPLTHEYPLANPGDPASGSFVSSALVAGDLFAR
                     VSLVFDQATWSGAWSDALSGSSSTATFNNTQYPIRVTNRGALTERWIVRLTNSTSFEV
                     IGENVGVIATGNTSADCAPNNPATGVPYFRLPALGWGNGWAT"
     assembly_gap    1365..1396
                     /estimated_length=32
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1397..>2508)
                     /locus_tag="PLV68_17205"
     CDS             complement(<1397..>2508)
                     /locus_tag="PLV68_17205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03040.1"
                     /translation="ILTWNVPTQETQQPQAALKASQALQLSPPEGKAVQPGSLSISWE
                     YGGTKTASAATSGMLSGAATGSLSVAQNRLDFAPNVLPSVGTQITVSYVAGPKQEDAF
                     AHPSRNGTGTLPVTATLGAIEPGSLEVEWNTLTDTAGLGAYTFAQLQEMGIAISIWRD
                     PTQIARDDGGGNVVLNGASIGTVNYATGEVIFNPDVTIRIPRPNYSLGAAIAGTGRWR
                     LNYGGISYVTAPSLYPNDESGYVKLRYNSAGATNNQTETFQFAPSFKLVPGVNAQVVT
                     GTVLLTTSGAQPWGDNGQGALREFTPSGWVTRGTINYLSGEVALTSWTAGVTNAITRA
                     SCVTTVGENISSEFVFRTGAAPLRPGSLSIQYARAV"
BASE COUNT          362 a          752 c          784 g          401 t
ORIGIN      
        1 catggtcgtc gtcaacacgc ggaacgatcc gttgtactcg ggctgctcgg ctcctgcgat
       61 ctcgaccacc tgaaatggcc gataggcgtg cccgctggaa atggtggccg tagcgatgcc
      121 atcggcgaag gtcagcgtgt cgatggcctt caaggcgaag ccgttgacga gacaggcgtc
      181 gagcatcgtc accagatcgc cccagttgtt gctgatctgc ggcgcgccgg tcnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc ggtggcccag
      421 ccgttgcccc agccgagcgc gggcaggcgg aagtacggca ccccggtcgc cgggttgttg
      481 ggcgcgcagt cggcgctggt gttgccggtg gcgatcacgc cgacgttctc gccgatgacc
      541 tcgaacgagg tgctgttggt caggcgcacg atccaacgct cggtcagtgc tccccgattg
      601 gtgacgcgga tcgggtactg cgtgttgttg aaggtcgcgg tcgacgagct gcccgacagc
      661 gcgtccgacc acgcgccgct ccaggtcgcc tgatcgaaca ccagactgac gcgggcgaac
      721 aggtcgccgg ccaccagcgc gctggaaacg aaactgcccg aggcgggatc accgggattg
      781 gcgagcggat actcgtgagt cagcgggcgc gtgaagctga tctcgccgtt gatctgcacg
      841 tcgcgcacca cggccatgtc ctcgatgcgg tgctcgacgg tcaccggctg gctgaagccc
      901 gtcacgttgg tgaatgtgac cgtgccggcc tcgagatcgg cggtgtagcc ggtgttgatc
      961 acagatccgt cgtggccgac cacgcgcacg cgcgacaggc gcacccgggc gcaatcgatg
     1021 gtctggccgt tgctgaccga ggtggtgatc ttgccggtgt ggccgacaac ggcgaagcca
     1081 cccggacgga agatcggtac tcgcccatcg ctgggcagcc gcacgggatc gatgccgagc
     1141 agatcggcat ccagcggcag atagctgtag gccactgcgc tgtagcgcac actggaggca
     1201 gccaccggct cgggccggaa gatcttgccg tccgtaccca cgcggtcggc ggcgtaccac
     1261 ggttcgctct cgttgcctgc cgccgtgacc gtcgtgccga agcgcacgcg caccaggcca
     1321 gtctcgtagt cgacgctgcc gttgatgccg gtcgcctcga tcttnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnngacc gcgcgggcgt actggatcga cagcgaccca ggacgaagcg
     1441 gtgccgcgcc agttcggaac acgaactcgc tggagatgtt ctcgccgacc gtggtcacgc
     1501 aactggcgcg cgtgatcgca ttggtcacac ccgccgtcca ggacgtgagg gccacctcgc
     1561 ccgacaggta gttgatcgtg ccgcgcgtca cccagccgct gggggtgaac tcgcgcaggg
     1621 cgccctgacc attgtcgccc cagggctgcg cgccactggt cgtgagcagc accgtgcccg
     1681 ttaccacctg ggcgttcacg cccggcacca gcttgaagct gggcgcgaac tgaaaggttt
     1741 ccgtttgatt gttggtcgcg cccgcgctgt tgtagcgcag cttcacgtag cctgattcgt
     1801 cgttcgggta cagcgacggc gcggtgacgt agctgatgcc accataattg agacgccagc
     1861 gcccggtgcc ggcgatggcc gcgccgaggc tgtagttcgg gcgtggaatc cggatcgtca
     1921 cgtccgggtt gaagatcact tcaccggtcg cgtagttgac cgtgccgatg ctcgcgccgt
     1981 tgaggacgac gttgccgcca ccatcgtcgc gggcgatctg cgtcgggtcg cgccagatcg
     2041 agatggcaat ccccatctct tgcaactgcg cgaaggtgta cgcgcccagc cccgccgtgt
     2101 cggtcagcgt gttccactcg acctccagcg aacccggttc gatggcaccg agcgtcgccg
     2161 tcaccggcaa cgtccccgta ccattgcggg aggggtgcgc gaaggcatcc tcctgcttgg
     2221 ggcccgccac atagctcacg gtgatctggg tgccgaccga cggcagcacg ttgggcgcga
     2281 agtcgaggcg gttctgcgcg acgctcaggc tgccggtggc cgccccggac agcatgccgg
     2341 acgtcgcagc cgacgcggtc ttggtaccgc cgtattccca gctgatggag agcgacccag
     2401 gttgcacggc cttgccttcc ggcggtgaga gctgcaatgc ctgcgaagcc ttgagggcag
     2461 cctgcggctg ctgcgtttcc tgcgtcggca cattccacgt caggatca
//
LOCUS       DAORNF010000524         2507 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_31445_length_2507_cov_1.667618, whole genome shotgun sequence.
ACCESSION   DAORNF010000524 DAORNF010000000
VERSION     DAORNF010000524.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31445_length_2507_cov_1.667618"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>752)
                     /locus_tag="PLV68_17210"
     CDS             complement(<1..>752)
                     /locus_tag="PLV68_17210"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRE03041.1"
                     /translation="TLPQIDQFVAEGWFVDNPEGFVQPRRPYRFDGEHFGPPPATDPI
                     GANSLADWTPRPASRPASHLASGATGDVLPLAGLRVADFTGFWAGPMASGILAGLGAD
                     VIHIEGPRRPDGIRLNSIRSMADDDWWEWSPLFCGANTNKRDLAIDLSVARGREIALR
                     LLATCDVMIENYSPRVVDQLGLGPDVAREANPDIIVVRMPAFGLDGPWRDRVGFAQTI
                     EQAVGLAFVTGYPGETPLIPNGMCDPLAGVFG"
     assembly_gap    753..762
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<763..1176)
                     /locus_tag="PLV68_17215"
     CDS             complement(<763..1176)
                     /locus_tag="PLV68_17215"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRE03042.1"
                     /translation="MSVLGVLPRLMSTGSGELLDVSSFECTTIEHGMHPVTFSSMAQR
                     PFQSSRGTPVPGIEPTSDGLVGFFVITGQQWLDFCSMIGRTDWADDERLFIATERRLR
                     ADELLGPIREWTTKMTTAEIVELASLMRIPVSPIGS"
     assembly_gap    1188..1248
                     /estimated_length=61
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1249..1558)
                     /locus_tag="PLV68_17220"
     CDS             complement(<1249..1558)
                     /locus_tag="PLV68_17220"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRE03043.1"
                     /translation="MAFVEPSEGHELRWRGSADGTSPLFAYLHAGKRSILEPDSFDEL
                     LAGTDVVVTDRLSEAWDALHARHPHLSVVVITPFGMHGPWADRPATDLTLQAASGGMA
                     P"
     gene            complement(1684..>2507)
                     /locus_tag="PLV68_17225"
     CDS             complement(1684..>2507)
                     /locus_tag="PLV68_17225"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR03617 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HRE03044.1"
                     /translation="MTLAQLAWDLQEFSGGRFLMGLGTQVEAHVTRRFSMPWAKPASR
                     MRELILAVRAIWATWRDGDALEFHGEFYRHDLMTPFFTPTSEIIAAVGLPPILLAGVG
                     PRMTEIAGEVCDGFICHGFTTERYLREVSIPALERGRSRAGKSLAGFEIVGPPFVVTG
                     QTDADRRASDARARQQLGFYASTPAYRAVLELHGWGALGVAANNEARKGRWSELGSLI
                     DDEVLDAFAIVGPLDEIADRIQHRYGDVITRFRFSAPDEDDIARWAHVAGALHDR"
BASE COUNT          416 a          816 c          837 g          367 t
ORIGIN      
        1 caccgaagac gcccgcgagc ggatcacaca tcccgttcgg gatcagcggc gtctcgcccg
       61 gatacccggt gacgaaggcc agcccgacgg cctgttcgat cgtctgcgcg aagccgaccc
      121 ggtcgcgcca cggaccatcg agcccgaagg cgggcatccg aacgacgatg atgtccgggt
      181 tggcctcgcg ggcgacgtca ggcccgagtc ccagctggtc gacaacccgc ggcgagtagt
      241 tctcgatcat gacatcgcag gttgcgagga gccggagtgc gatctcccgt ccccgcgcga
      301 cgctgagatc gatcgcaaga tcgcgcttgt tcgtgttggc tccgcagaac aacggggacc
      361 attcccacca gtcgtcgtct gccatcgagc ggatcgagtt gagccggatc ccgtcgggcc
      421 gccgcggccc ttcgatgtga atcacgtcgg cgccgagccc ggccaggatg ccgctggcca
      481 tcggcccggc ccagaagccg gtgaagtcgg cgacgcgaag ccctgccaac gggagcacgt
      541 cgcctgtcgc cccagacgcc aggtgtgatg caggtcgcga tgccggtcgc ggggtccagt
      601 cggcgagtga gttggccccg atcggatccg tcgcgggcgg cggtccgaag tgttcaccgt
      661 cgaatcgata cggccgccgc ggctggacga atccctcggg gttgtcgacg aaccaaccct
      721 cggcgacgaa ctggtcgatc tggggcagcg ttnnnnnnnn nnggagccga tcggggagac
      781 cgggatgcgc atcaacgaag ccagctcgac gatctcggcg gtcgtcatct tcgtcgtcca
      841 ctcgcggatc ggtccgagca gctcgtcggc gcgcaggcgc cgctcggtgg cgatgaacag
      901 tcgttcgtcg tcggcccaat cggtacgacc gatcatggag cagaagtcga gccactgctg
      961 cccggtgatc acgaagaagc ctacgaggcc gtcggatgtc ggctcgatcc cagggaccgg
     1021 agtgccccgc gacgattgga acggccgttg cgccatcgac gagaacgtca cggggtgcat
     1081 gccatgttcg atcgttgtgc attcgaagct ggagacgtcg agcaactcac ccgaccccgt
     1141 cgacatcagc cgcggcagaa caccaagcac cgacacagcc gcgaccgnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnga ggagccatcc
     1261 ctccggacgc cgcctgcagg gtcagatcgg tggccgggcg atcggcccac gggccgtgca
     1321 tgccgaacgg ggtgatgacc accaccgaca ggtgaggatg acgcgcgtgc agcgcgtccc
     1381 acgcctcgct cagccggtcg gtgaccacga cgtctgttcc ggcgagcagc tcgtcgaacg
     1441 agtcgggctc gaggatgctg cgcttgcccg cgtgcaggta cgcgaacagc ggtgaggtgc
     1501 cgtcggccga tccacgccag cgcaactcgt gaccttcgct gggctccacg aacgccacgt
     1561 ccgcaccggt gtcggcggat cttcgtacag aacgcgacgt cgatcccgcc gttccattgc
     1621 agcacccgca ccccgtcgtt cggagcgacg agtgatggat gcggcccggt gctggcccgc
     1681 tcgtcaacgg tcatggagcg ctccggcgac gtgcgcccac cgggcgatgt cgtcctcgtc
     1741 gggtgccgag aagcggaagc gggtgatgac gtcgccatat cggtgctgga tccgatcggc
     1801 gatctcgtcg agcggaccca cgatcgcgaa cgcatcgagt acctcgtcat cgatcagcga
     1861 gcccaactcg gaccagcggc ccttgcgagc ctcgttgttg gcggcgacgc cgagcgcgcc
     1921 ccaaccgtgc agttcgagca ccgctcgata cgcaggagtc gacgcataga agccgagctg
     1981 ttggcgggcc cgagcatcgc tggcgcgacg gtcggcgtcg gtctggccgg tgacgacaaa
     2041 gggagggccg acgatctcga atccggccaa cgacttgccg gcgcggctgc gacctcgctc
     2101 gagcgcaggg atcgagacct cgcgcaggta ccgctcggtg gtgaacccgt ggcagatgaa
     2161 cccgtcgcac acttcaccgg cgatctcggt catccgtggc ccgacgccgg cgagcaggat
     2221 cggggggagg ccgaccgcgg cgatgatctc tgaggtgggg gtgaagaacg gggtcatcag
     2281 gtcgtggcgg tagaactcgc cgtggaactc cagcgcgtcg ccgtcacgcc acgtcgccca
     2341 gatcgcgcgg acggccagga tgagttcgcg catgcggctg gccggcttgg cccacggcat
     2401 gctgaaccgc cgcgtgacgt gtgcttccac ctgggtgccg agacccatca ggaaccgtcc
     2461 gccgctgaac tcctgcagat cccacgcgag ttgggcgagc gtcatcg
//
LOCUS       DAORNF010000525         2502 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_31523_length_2502_cov_1.497752, whole genome shotgun sequence.
ACCESSION   DAORNF010000525 DAORNF010000000
VERSION     DAORNF010000525.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31523_length_2502_cov_1.497752"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    281..379
                     /estimated_length=99
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <380..820
                     /locus_tag="PLV68_17230"
     CDS             <380..820
                     /locus_tag="PLV68_17230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03045.1"
                     /translation="VTWVLHDESGLYTKQNKLRKVAENQRRGAAGMGGRTLETTNAWD
                     PAEESTAQLTFESQRPDILKFFRRAPVELDYLDPVQRRQIHEFVYAGCWWVDLDAIEA
                     EAAELIEKDPAQAQRFYGNMLVAGAGSWMPEALWASAARVAAVA"
     assembly_gap    834..843
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <844..1432
                     /locus_tag="PLV68_17235"
     CDS             <844..1432
                     /locus_tag="PLV68_17235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03046.1"
                     /translation="EPVCLGFDGSDSDDWTAIRAETLDGFQFTPTYGPDRRPTYWAPE
                     EWPGHRIPRHEVHAAMAELFDRYAVERAYCDPHDWQTEIEGWALRFGEEHVFRWDTGR
                     GNTRVTAVWYALERFKTDLATGVLTHDGCPMTAVHMRNTRKVAKPGEKYTIGKPAQHQ
                     KIDLAMTSVLCHEAACDARSAGWTKPERALTVVWR"
     gene            1434..>2143
                     /locus_tag="PLV68_17240"
     CDS             1434..>2143
                     /locus_tag="PLV68_17240"
                     /inference="COORDINATES: protein motif:HMM:NF016988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HRE03047.1"
                     /translation="MEDPEKTLMRLMKLHSVDRRDIALLDLYYEGEQPLSLLAPALHD
                     ELGGRISDVVINWPRMIADAYEHRLDVTFLRMPDVNDTRDNLLRDMLTENAWTRLQSE
                     VHTEALACKRSYVIVGAGENHGDAPVVTAESPLQVFARHDPKTRKVVEAVKVWSDDDR
                     VKWASVYQMGLRTTWRRERAGKWILDESDEHGLDSTLVTEFVNRGRVTRRGGVSEFQD
                     IIPLANAANKMATDMMVSG"
     assembly_gap    2144..2230
                     /estimated_length=87
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2231..>2502
                     /locus_tag="PLV68_17245"
     CDS             <2231..>2502
                     /locus_tag="PLV68_17245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03048.1"
                     /translation="AGRIWSTNKKPGDVAYGQFEESDLAVFHNTIKVLAQLAGQLANL
                     PQDYMAFTSANPTSADGQRASETRMIKRAERMQGNFGPSWAHVARD"
BASE COUNT          397 a          646 c          879 g          384 t
ORIGIN      
        1 cgcagatcgt gaagtcgcag aagacgggga agggtccgta ctccgccgct gtgtgttgca
       61 cggaggcggt gggcccgtcg gtgttcgcgg gttgggcccg tccgggtgac gcgtacgagt
      121 gcgcggatca cggctgccag tgcggctggg tgtacgagta tcagccgggc gaggcgaagg
      181 cgattccgcg gccgacgccg ctgatccagt tgatggcgac gtcggaggac cagatcctgg
      241 acaacgtcta cgagccgttg aaggccatga ttcgtggcgg nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnng tgacgtgggt gctgcatgac gagtccggcc tgtatacgaa
      421 gcagaacaag ttgcggaagg tcgccgagaa ccagcgtcgt ggcgcggcgg gtatgggtgg
      481 ccggacgttg gagacgacga acgcgtggga ccctgcggag gaatcgacgg cccaactcac
      541 gttcgagtcg cagcggccgg acatcctcaa gttcttccgc cgggcgccgg tcgagctcga
      601 ctatctcgat ccggtgcagc gccgccagat ccacgagttc gtgtacgccg ggtgctggtg
      661 ggttgacttg gacgcgattg aggcggaggc cgccgagttg atcgagaagg acccggcgca
      721 ggcgcaacgg ttttacggca acatgctggt ggcgggtgcc gggtcgtgga tgccggaggc
      781 gttgtgggcg tcggctgcca gggtggcggc ggtcgcgtga gtacgccccg ctgnnnnnnn
      841 nnncgagccg gtgtgtctcg ggttcgacgg ttcggactct gatgactgga cggcgatccg
      901 ggctgagacg ttggacgggt tccagttcac cccgacttac gggccggatc ggcggccgac
      961 ctattgggct ccggaggagt ggccggggca tcgcatcccg cggcacgagg ttcacgcggc
     1021 gatggccgag ttgttcgatc ggtacgcggt ggagcgggcg tattgcgacc cgcacgactg
     1081 gcagaccgag atcgagggtt gggcgttgcg gttcggtgag gagcacgtgt tccggtggga
     1141 caccggccgg ggcaatacgc gggtgacggc ggtttggtac gcgctcgagc ggttcaagac
     1201 ggacctggcg accggtgtgc tgactcacga cgggtgcccc atgaccgcgg tgcatatgcg
     1261 gaacacgcgc aaggtcgcga agccgggcga gaagtacacg atcgggaagc cggcgcaaca
     1321 tcagaaaatc gacctggcga tgacgtcggt gttgtgccat gaggcggcgt gtgatgcgcg
     1381 gtcggctggt tggacgaagc ccgagcgggc tctgacggtg gtgtggaggt gacgtggaag
     1441 atcccgagaa gacgctgatg cggttgatga agctgcactc ggtcgaccgt cgcgacatcg
     1501 cactcctgga cctgtactac gagggtgagc agccgctgtc gttgttggcg ccggcgttgc
     1561 acgatgagct cggcggccgc atttcggatg tcgtgatcaa ctggccgagg atgatcgctg
     1621 acgcgtacga gcatcggctc gacgtcacgt ttctgcggat gccggacgtc aacgacacgc
     1681 gcgacaacct gctgcgggac atgctcaccg agaacgcgtg gacccggctg cagtcggagg
     1741 tgcacaccga ggcgttggca tgcaagcggt cgtacgtgat cgtcggtgcc ggcgagaacc
     1801 acggcgatgc tccggtggtg acggctgagt cgccgctgca ggtgttcgcc cgtcacgacc
     1861 cgaagacccg caaggttgtc gaggccgtga aggtgtggag cgacgacgac cgggtgaagt
     1921 gggccagcgt ctaccagatg gggctgcgca ccacctggcg tcgcgagcgg gcgggcaagt
     1981 ggatcctcga cgagtcggac gagcacgggc tcgactccac gctcgtcacc gagttcgtca
     2041 accgtggccg ggtgacccgc cgtggcgggg tgtccgagtt ccaggacatc atcccgttag
     2101 cgaacgcggc gaacaagatg gcgacggaca tgatggtgtc gggnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn gcgggtcgga tctggtcgac gaacaagaag ccgggcgatg tcgcctacgg
     2281 ccagttcgag gagtccgacc tggcggtgtt ccacaacacg atcaaggtcc tcgcgcaact
     2341 ggctggccag ctggcgaatc tgccgcagga ctacatggca ttcacctcgg ccaacccgac
     2401 atcggcggac gggcagcggg cgtcggagac gaggatgatc aagcgtgcgg agcgcatgca
     2461 gggcaacttc gggccatcct gggctcacgt cgcccgggac at
//
LOCUS       DAORNF010000526         2492 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_31676_length_2492_cov_2.243742, whole genome shotgun sequence.
ACCESSION   DAORNF010000526 DAORNF010000000
VERSION     DAORNF010000526.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31676_length_2492_cov_2.243742"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>218)
                     /locus_tag="PLV68_17250"
     CDS             complement(<1..>218)
                     /locus_tag="PLV68_17250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HRE03049.1"
                     /translation="ARIEDLMQELKRDFTIVIVTHNMQQAARVSDRTAFYTTEVNPES
                     DRRTGMLVEFGATRTLFSTPADPRTEQY"
     assembly_gap    219..336
                     /estimated_length=118
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<337..936)
                     /locus_tag="PLV68_17255"
     CDS             complement(<337..936)
                     /locus_tag="PLV68_17255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRE03050.1"
                     /translation="MNDRTEVIDMLDTFDEGEQRVPDPGSGSASPSSASPGSASPGSA
                     SVVVPGATTGPAAVFAVRDLNVYYGAHRAVRDVNMTIYEHEITAFIGPSGCGKSTVLR
                     CFNRMNDLIDIARVEGQVEYHGVDLYHPDVNAVEVRRRIGMVFQKPNPFPKSIYDNVA
                     YGPRLAGVRKRSDLDDIVEKSLRGAALWDEDGGRLKSSAY"
     gene            1205..2419
                     /gene="pstS"
                     /locus_tag="PLV68_17260"
     CDS             1205..2419
                     /gene="pstS"
                     /locus_tag="PLV68_17260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HRE03051.1"
                     /translation="MNTTNKRSAIVAGVLSLTLLAAACGSDSDSNSTTTTAASSTTNE
                     ATTTSAATSTTAGPSTSADQTSSSAAVPSTDAGTEFDYAALQGTLVGSGATFPKAFYD
                     EAIAYLADIAPDLTVEYGGGGSGKGRSDLQEQVVNFAGTDSTVKAEDLEKFKGGEFVY
                     IPTVIAPITVSYNVPGVDELHLTPATIAKIFQLEVTTWNDPAIAADNPDADLPDTAIV
                     VARRADGSGTTDNFSKFLNAAVGPDATGEWTLGSGSELEWPAGTQAGDGNSGVAQIIK
                     STEGAIGYVDLSDAVANGFTFAAVQNKAGEFVLPTIEAATAAAAGATIKDDLTFFVGW
                     ADGAEAYPIAAQTWIIAYTTQDDAEAAENLRGFLTYLLTEGQEWAPEVDFAPLPEQLR
                     QQALENVAKIGA"
BASE COUNT          409 a          836 c          741 g          388 t
ORIGIN      
        1 catactgctc ggtccgcggg tccgcggggg tggaaaacaa tgtacgggtc gcaccgaatt
       61 caaccagcat gcccgtgcgg cgatcgctct ccggattgac ctcggtggtg tagaacgcgg
      121 tgcggtcgct gacccgggcg gcctgctgca tgttgtgggt cacgatcacg atcgtgaagt
      181 cacgcttcaa ctcctgcatc agatcttcga tgcgtgccnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngtag gccgacgact tcaaccgtcc
      361 gccgtcctcg tcccacagcg ccgcgccacg cagcgacttc tccacgatgt cgtcgagatc
      421 gctgcgtttg cgcacgcccg ccaggcgtgg tccgtaggcg acgttgtcgt agatgctctt
      481 cggaaacggg ttcggcttct ggaacaccat cccgatccgg cgccgcacct ccaccgcgtt
      541 gacatccggg tggtagaggt cgacgccgtg gtactcgacc tggccctcca cgcgggcgat
      601 gtcgatcagg tcgttcatcc ggttgaaaca ccgaagcacg gtgctcttgc cgcagcccga
      661 cggcccgatg aacgcggtga tctcgtgctc gtagatcgtc atgttcacgt cacgcaccgc
      721 ccggtgggcg ccgtagtaga cgttgaggtc gcgcaccgcg aacactgccg ccgggccggt
      781 cgttgcaccc ggcacgacga ccgatgcgga gcctggcgat gcggagcctg gcgatgcgga
      841 gcttggcgat gcagagcctg agcccgggtc tggcacccgt tgctcgccct cgtcgaacgt
      901 gtcgagcatg tcgatcacct cggtcctgtc gttcatctgc tgttgggtcg attgcatcgc
      961 ttcacctcgg gtgcagaccc tggtgggcct cgcgccgatg ggcaccgtaa gaggccaagg
     1021 tgaccagggc tcggagcctg ggtgaatgac gggtgaacag caggcgaagt gcgggcggcg
     1081 cgaccggtgc gacgccgacg attcgccagt cgttcatcca ccgttcaacg tgtctcagga
     1141 tgccgtccac cttggctcct tacggtttct ggcggttgca ctcacccctc aaggagaacc
     1201 acacgtgaac acgaccaaca agcgctcggc gatcgtcgcc ggtgtgttgt ccctcacctt
     1261 gctcgccgca gcgtgcggca gcgacagtga ttcgaacagc acgacgacca ccgctgcgtc
     1321 cagcaccacc aacgaagcca cgacaaccag cgccgccaca tcgacgacag ctgggccgtc
     1381 gacctcggcc gatcagacct cgtcgagcgc cgccgtgccc agcaccgacg ccggaaccga
     1441 gttcgactac gccgcgctgc agggcactct cgtcggctcc ggggccacct tccccaaggc
     1501 gttctacgac gaagcgatcg cctatctcgc cgacatcgcc cccgacctca ccgtcgagta
     1561 cggcggcggc ggttcgggca agggccgctc cgacctgcag gaacaggtcg tcaacttcgc
     1621 cggcaccgac tcgaccgtca aggccgagga cctggagaag ttcaagggtg gcgagttcgt
     1681 ctacatcccc accgtgatcg ccccgatcac cgtgtcgtac aacgtgccgg gcgtcgacga
     1741 actccacctc acccccgcca ccatcgccaa gatcttccag ttggaagtga cgacgtggaa
     1801 cgatccggcg atcgcggccg acaacccgga tgccgacctg cccgacacgg cgatcgtcgt
     1861 cgcccgccgc gccgacggtt cgggtacgac cgacaacttc tccaagttcc tcaacgccgc
     1921 cgtcggcccg gatgcgaccg gcgagtggac gctcggcagc ggttccgagc tggagtggcc
     1981 cgctggcacc caggccggcg acggcaacag cggtgtggcc cagatcatca agagcaccga
     2041 aggtgcgatc ggctacgtcg acctcagcga tgcggtcgcc aacggcttca ccttcgccgc
     2101 cgtgcagaac aaggccgggg agttcgtgct gccgacgatc gaggcggcca ccgccgccgc
     2161 ggccggtgcc accatcaagg acgacctgac gttcttcgtc ggttgggccg acggtgccga
     2221 ggcctacccg atcgcggcac agacctggat catcgcctac acgacccagg acgacgccga
     2281 ggccgcggag aacctgcggg gcttcctcac ctacctgctg accgaaggcc aggagtgggc
     2341 accggaggtc gacttcgctc ccctgcccga gcagcttcgc cagcaagcgc tggagaacgt
     2401 cgccaagatc ggcgcctgag tggctctcac actctccgat ctccgcagcc gacgacagct
     2461 cggggaccgc gccttccgtg ccgccaccat cg
//
LOCUS       DAORNF010000527         2490 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_31715_length_2490_cov_1.778234, whole genome shotgun sequence.
ACCESSION   DAORNF010000527 DAORNF010000000
VERSION     DAORNF010000527.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2490
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31715_length_2490_cov_1.778234"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..655
                     /locus_tag="PLV68_17265"
     CDS             <1..655
                     /locus_tag="PLV68_17265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c-type biogenesis CcmF C-terminal
                     domain-containing protein"
                     /protein_id="HRE03052.1"
                     /translation="FAAGAAGRQLVLATRRQGWRGLVGRANGGMVVHLGIIVVAVALV
                     ASTSYTHSSRLLVQPGVPVSYGGHTFEVIELQRFETARTNGIKAQISIDGGQTYAPAL
                     TTYTSHGMVVPTPSVRTGLYEDIYLALEPGATLDAPTVAIKVFVKPMIIWLWIGGLMT
                     VVGTLLSAYPGQRRRRPTDPTSAPIDLGDGDSGGDRGSGGDRDGGDADESREELAGV"
     gene            648..1253
                     /locus_tag="PLV68_17270"
     CDS             648..1253
                     /locus_tag="PLV68_17270"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HRE03053.1"
                     /translation="MSEASTAAPTATRRGRVAPIIVLAVAVAMGGLFWLLIGAKSGDG
                     ADSNYTPLLDKAAPAVKTTTLDGQTFDLQRRKGSWVVLNFFQSTCVPCVREHPELIAF
                     AEQQADRGVYGAELYTVVSLGDPIDKVTEFFAANGGTWPILRDDDANIQVSFGVYKVP
                     ETWIIDPDGRVVFRTIAQVTADSLTRELGVQRSLYEQRVSG"
     gene            1250..1744
                     /locus_tag="PLV68_17275"
     CDS             1250..1744
                     /locus_tag="PLV68_17275"
                     /inference="COORDINATES: protein motif:HMM:NF015853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HRE03054.1"
                     /translation="MSQRPWNRAVKSRSGWIVLGLLVVVALVFGAMRDTGPLTDGERA
                     DEIAKQLACPVCAGESVFESQSNQALAIQRQITSLVQQGVYSDDEIIAYLSEQFDART
                     QLVPKGEGVEALVWVVPVIAGVAAVAGLAVAFRRWRLAADTVPTEADRALVAAALAGQ
                     DEDI"
     gene            1747..>2490
                     /locus_tag="PLV68_17280"
     CDS             1747..>2490
                     /locus_tag="PLV68_17280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03055.1"
                     /translation="MARIDLDQLAELEEERRFLLTSLADLERERAAGDVDDTDYLVLH
                     DGYTARAATVLRAIGEQHAALPPKRPHSLKRTLIGIGITMAVAGALVAAVLQFSAPRG
                     DGDTITGNQTGDRVASLLTEAFSALSSDQQRAIDAYREVLEIEPSNVEAKTYLGWVLA
                     LASQNQGDATGGEQVLESALATIDDAIEIDPTYADPYCFRAIIAVRMQGDLAGGAGYE
                     QQCLDHRPGADLRSLVDQEVTTPIKVAALV"
BASE COUNT          392 a          841 c          872 g          385 t
ORIGIN      
        1 gttcgcggct ggtgcagccg ggcgtcagtt ggtgctggcc actcggcgcc agggttggcg
       61 cggcctggtc gggcgggcca acggcggcat ggtcgtgcat ctcggcatca tcgtggtcgc
      121 ggtcgccctg gtggcatcca cctcctatac gcactcgtcg cggctgctcg tgcagccggg
      181 cgtgcccgtc agctacggcg gtcacacctt cgaggtgatc gagctgcagc ggttcgagac
      241 ggcccgcacc aacggcatca aggcccagat cagcatcgac ggcggccaga cctacgcccc
      301 ggcactgacc acctacacca gccacggcat ggtcgtgccg acgccgtcgg tgcgcaccgg
      361 tctctacgaa gacatctacc tcgcgctgga gccaggggcc acgctcgacg cgccgacggt
      421 cgcgatcaag gtgttcgtca agccgatgat catctggctg tggatcgggg ggctgatgac
      481 cgtggtcggc acgctgctgt cggcttaccc gggccagcgt cgccgtcgcc ccaccgatcc
      541 gacctcggct ccgatcgacc tcggtgatgg cgacagcggc ggtgatcgtg gcagcggcgg
      601 cgatcgtgac ggcggcgacg ccgacgagtc gcgagaggaa ctggccggtg tctgaggctt
      661 ccactgccgc tcccacggct acccggcgtg gtcgggtcgc gccgatcatc gtgctggcgg
      721 tcgccgtggc gatgggcggt ctgttctggc tgctgatcgg cgccaagtcg ggtgacgggg
      781 ccgactccaa ctacacgccg ctgctcgaca aggccgcccc ggcggtcaag accaccaccc
      841 tcgacggcca gaccttcgat ctgcaacgac gcaagggcag ttgggtggtg ctcaacttct
      901 tccagagcac ctgcgtgccc tgcgtccgcg agcaccccga gctgatcgcc ttcgccgagc
      961 agcaagcgga ccgtggtgtc tacggcgccg agctgtacac ggtggtctcg ctcggtgatc
     1021 cgatcgacaa ggtcacggag ttcttcgcgg ccaacggcgg gacctggccc atcctgagag
     1081 acgacgacgc gaacatccag gtcagcttcg gcgtctacaa ggtgcccgag acctggatca
     1141 tcgatcccga cggtcgcgtc gtgttccgca cgatcgccca ggtcaccgcc gactcgctca
     1201 cccgcgagct cggcgtgcag cgcagcctgt acgaacagcg ggtcagcgga tgagccagcg
     1261 gccgtggaac cgggcggtca agagccggtc gggttggatc gtgctcggcc tgctcgtcgt
     1321 ggtggccttg gtgttcggcg cgatgcgcga caccggcccc ttgaccgacg gcgaacgggc
     1381 cgacgagatc gccaagcaac tggcctgccc ggtgtgcgcg ggcgagagcg tgttcgagtc
     1441 gcagtcgaac caggcgctgg caatccagcg ccagatcacc tcgctggtgc agcagggcgt
     1501 ctacagcgac gacgagatca tcgcctacct gtccgagcag ttcgacgccc gcacccagtt
     1561 ggtgcccaag ggcgaaggtg tcgaagcgct ggtgtgggtg gtgccggtga tcgccggtgt
     1621 cgccgccgta gccgggctgg ccgtcgcctt ccgccgttgg cgcctggcgg ccgacacggt
     1681 gccgaccgag gccgaccggg cgctggtcgc cgccgcgctc gccggtcagg acgaggacat
     1741 ctgacgatgg cccggatcga cctcgaccaa ctcgcggagt tggaagagga gcgccgcttc
     1801 ctgttgacct cgttggccga cctcgagcgc gagcgtgccg cgggtgatgt cgatgacacc
     1861 gactacctcg tgctgcacga cggctacacg gcgcgggcag ccacggtgct gcgcgcgatc
     1921 ggcgagcagc acgcggccct accgcccaag cgtccgcaca gcctcaagcg caccctgatc
     1981 gggatcggca tcacgatggc cgttgccggt gcgctggtcg cggcggtact gcagttcagc
     2041 gcgccacgcg gcgacggcga cacgatcacc ggcaaccaaa ccggcgaccg ggtggcgtcg
     2101 ctgttgacgg aagcgttcag cgcgctgtcc agcgatcagc agcgagcgat cgacgcctac
     2161 cgtgaggtgt tggagatcga gcccagcaac gtcgaagcca agacatacct cggctgggtg
     2221 ttggcgttgg cgtcgcagaa ccaaggcgac gcgacgggtg gcgagcaggt cttggagtcg
     2281 gcgctggcca ccatcgacga cgcgatcgag atcgacccga cctacgccga cccctactgc
     2341 ttccgggcga tcatcgcagt gcggatgcag ggcgacctgg ctggcggcgc ggggtacgag
     2401 cagcagtgcc tcgatcacag accgggggcc gacctgcgca gcctggtcga ccaagaggtc
     2461 accacgccga tcaaggtcgc cgcgctggtg
//
LOCUS       DAORNF010000528         2482 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_31836_length_2482_cov_1.833951, whole genome shotgun sequence.
ACCESSION   DAORNF010000528 DAORNF010000000
VERSION     DAORNF010000528.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2482
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31836_length_2482_cov_1.833951"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(182..1435)
                     /locus_tag="PLV68_17285"
     CDS             complement(182..1435)
                     /locus_tag="PLV68_17285"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HRE03056.1"
                     /translation="MTTTATPSGSSAASRGIRYPHPIDYSLYNWPTFSDAEFARRHQI
                     VRDFMDEHGLEALLITGNSAIWERGWANIRWVSNFMGTMELDCTCVFPRHGDPMLAIL
                     GLNARLPDRLARSVISDIRGGLNTAPLIVDYIRELGISSGRIGIIPPAPYIDINHDAA
                     VALNEAFPNIEFPRFSDEFWRKRMVLSEEELACIDEAGRIGDAAVQAIIDRLRPGMRE
                     QDLFNIIYDSFSVEGGENPCMVLAASENMFHPTSAFQRPRPIGREIQQGDVLLLELGA
                     RDGHGYEAQTGKPIIYGPPPKDYAAMLDTCLEAYHAIADVLKPGCSAADIRKAGSVIT
                     ERGYTVVAPLVHGVFNPIDAGPFVGTSHRPDKDVILEPGMGVCVEIHPCSEDIIRGVF
                     LGDTFVITEDGARCVNKLEAKVYQL"
     gene            complement(1484..>2482)
                     /locus_tag="PLV68_17290"
     CDS             complement(1484..>2482)
                     /locus_tag="PLV68_17290"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HRE03057.1"
                     /translation="IGASGGHEFYRGVVAKQIAAHIAARGGVLSFDDLDRHAGSWVDP
                     LSVDYHGNTVFSNAPVSMGALLLACLQVVELRLAERPWGDAAAQIDELVRLKRVAFGT
                     ILPLLGDPAHVSFDDVLSPAATRAWSAAVDGDLPPAPDEGGTDTTSLAVAAADGSMVV
                     FIHSLFNEFGSREYVPDAGIVLNDRLANLRRGEHPNALRAGCRPMHTLHAYLVDTAGG
                     ATIAGATPGGRGQVQTNLQVLLGVLDQGRDLQAAVDVPRWVHGMPRVAPDDQTLYLEP
                     ELIPLEPPLHALGCATEIVDAELHDHFGNCTIVGRDAEGTLSAASDHRRAGAAEAW"
BASE COUNT          407 a          794 c          879 g          402 t
ORIGIN      
        1 cggtgatctt cccgccgatc accgcgatgt acagccaccc cgccacggtg ggcgacctgg
       61 tcgaccacac cgtcatgcgg gtgtgcgatc agctcggctt cgagctggac atcgcaccac
      121 gctggggtca gcccgatcgc tgatccaacg gcggctgccc caacatcggc tgacggcaac
      181 gtcacagctg gtagaccttc gcttccagct tgttcacgca gcgggcaccg tcttcggtga
      241 tgacgaaggt gtcgcccagg aagacaccac ggatgatgtc ctcgctgcac gggtgaatct
      301 cgacgcagac gcccatcccc ggctcgagga tgacgtcctt gtcgggccgg tgcgaggtgc
      361 cgacgaacgg gccggcgtcg atcgggttga acacgccgtg cacgagcggc gcgaccacgg
      421 tgtagccgcg ctcggtgatg accgagcccg ccttgcggat gtcggccgcg ctgcacccgg
      481 gcttgagcac gtcggcgatc gcgtggtacg cctcgagaca ggtgtcgagc atcgccgcgt
      541 aatccttcgg cggcgggccg tagatgatcg gcttgccggt ctgggcttcg tagccgtgac
      601 cgtcgcgggc gccgagctcg agcagcagga cgtcgccctg ctggatctct cggccgatcg
      661 gacgggggcg ctggaacgcg ctggtgggat ggaacatgtt ctcggacgcg gccagcacca
      721 tgcacgggtt ctcgccaccc tcgacgctga acgagtcgta gatgatgttg aacaaatcct
      781 gctcgcgcat gcccgggcgg agccggtcga tgatcgcctg cacggccgcg tcaccaatgc
      841 gacctgcctc gtcgatgcag gccagctctt cctcgctgag caccatccgc ttgcgccaga
      901 actcgtcgct gaagcggggg aactcgatgt tgggaaacgc ctcgttcagt gcgaccgcgg
      961 cgtcgtggtt gatgtcgatg tacggcgcgg gcgggatgat gccgatgcga cccgagctga
     1021 tgccgagctc gcggatgtag tcgacgatca acggtgcggt gttcaaccca ccgcggatgt
     1081 ccgagatgac cgagcgggcc aagcggtcgg gcaggcgagc gttgagaccg aggatcgcca
     1141 gcatcgggtc gccgtggcgg gggaacacac acgtgcagtc gagctccatc gtgcccatga
     1201 agttggacac ccagcggatg ttggcccagc cgcgctccca gatcgcgctg ttgccggtga
     1261 tcagcagcgc ttccagaccg tgctcgtcca tgaagtcgcg cacgatctgg tggcgacgag
     1321 cgaactcggc gtctgagaag gtcggccagt tgtagaggct gtagtcgatg gggtgggggt
     1381 agcgaatacc gcgggatgcg gccgagcttc cagatggtgt tgcggttgta gtcatatcaa
     1441 cctcctagtt gaaaggcgat cgtgatggag gagcgccagg cgttcaccac gcctccgcgg
     1501 ctccagctcg acggtggtcg ctcgcggccg acagcgttcc ctcggcatcg cggccgacga
     1561 tcgtgcagtt gccgaagtgg tcgtgcaatt cggcgtcgac gatctcggtc gcacagccga
     1621 gcgcgtgcag tgggggctcg agcgggatca gttcgggttc gaggtacagc gtctggtcgt
     1681 cgggggcgac gcgcggcatg ccgtgcaccc agcgcggcac gtcgacggcg gcttgcaggt
     1741 cgcggccttg gtccagcacg ccgagcaaca cctgcaggtt cgtctggacc tggccgcgac
     1801 cacccggagt ggctccagcg atggtcgcgc cgccagccgt gtcgaccaga taggcatgca
     1861 gcgtgtgcat cgggcggcac ccggcgcgca acgcgttggg atgctcgccg cggcgcaggt
     1921 tggcgaggcg atcgttgagc acgatgcccg cgtcgggcac gtactcgcgc gaaccgaatt
     1981 cgttgaacag tgagtggatg aacaccacca tcgatccgtc ggcggcagcg accgcgagcg
     2041 acgtggtgtc ggtgccgccc tcgtcggggg ccggtggcag gtcgccgtcg accgcagcgc
     2101 tccacgcacg ggtcgcggcc ggggacaaca cgtcgtcgaa gctgacgtgg gcggggtcac
     2161 cgaggagggg caggatcgtg ccgaacgcga cgcgcttgag gcggacgagc tcgtcgatct
     2221 gtgccgcggc gtcgccccac gggcgctccg ccagacgcag ttcgaccacc tgcaggcagg
     2281 ccagcaacag cgcgcccatc gacacgggag cgttggagaa cacggtgttg ccgtggtagt
     2341 cgaccgacaa cgggtcgacc cacgagcccg cgtggcgatc gagatcgtcg aacgacaaca
     2401 cgccgccgcg cgccgcgatg tgcgctgcga tctgcttcgc gaccacgccg cggtagaact
     2461 cgtgaccacc gctggccccg at
//
LOCUS       DAORNF010000529         2481 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_31849_length_2481_cov_1.967848, whole genome shotgun sequence.
ACCESSION   DAORNF010000529 DAORNF010000000
VERSION     DAORNF010000529.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31849_length_2481_cov_1.967848"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..926)
                     /locus_tag="PLV68_17295"
     CDS             complement(<1..926)
                     /locus_tag="PLV68_17295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HRE03058.1"
                     /translation="MSYVYAFSHKHRRPPMEYKDLLGGKGANLAEMTSVLKLPVPPGF
                     TISTDACRAYMHSGWPTGLDDEIATQVAALERQMGRTLGNPSDPLLVSVRSGAKFSMP
                     GMMDTILNLGLNDKSVKGLAAVTGNERFAYDSYRRFISMYGRIVLDVPGEPFEHEFEA
                     VKEAAGVTNDAAISAADLKKLCETYKAIVKAHTGKAFPQDPTKQLRGAVEAVFRSWNG
                     ARAIAYRVRERISHELGTAVNVQTMVFGNRDDNSGTGVGFTRNAATGENKPYGDFLVN
                     AQGEDVVAGIRNTEDLDALKLQFPAIHTELLD"
     gene            986..2254
                     /locus_tag="PLV68_17300"
     CDS             986..2254
                     /locus_tag="PLV68_17300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03059.1"
                     /translation="MATRDNAAHGGSRGLHVFGFPVDVRPGFVFLLVLFGVVYSGRGQ
                     GELGLWLAVFVAAFTLAHELGHAFAARATGANASIALDFLAGYASFEPVRPLKRWERA
                     GISVAGPAVQIALGSIVVVALGANPLSYDSINDSPATFAAWWAGPIIGLINLIPILPL
                     DGGNIVMSGLDVVLPGRSRTVMLYFSLAVTIGAIVYFTLHGSLSPIFFFFFPLFSQLQ
                     MLQYRHAHTAIDKHGSWQLWAAASEREAWTIAKPGTFPPGIVASPYFRAAQLARRGQL
                     DAARDLLLSDFQSDRTPNWMPPDAASPREMAALIELLGTPLPTGNPYAEYVLATQLVR
                     LGEYEPAGLYAASSYGRSPGTMTAVVVAQCAAALGDDELAVSWLRAASAIGTNSAGLL
                     DAIDERIEFATIRHRPDVARLRQDVVASAA"
     gene            2299..>2481
                     /locus_tag="PLV68_17305"
     CDS             2299..>2481
                     /locus_tag="PLV68_17305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03060.1"
                     /translation="MSPFWCPITLDVVDVSGADAATFLQSQLSQNLTGLTVGQAVYSF
                     VLQPAGKLDALVRVRRV"
BASE COUNT          347 a          840 c          846 g          448 t
ORIGIN      
        1 atgtcgagca actcggtatg gatcgccggg aactgcagct tgagcgcgtc gaggtcttcg
       61 gtgttgcgga tgccggcgac gacgtcttca ccctgggcgt tgaccaggaa gtcgccgtac
      121 ggcttgttct cgccggtggc cgcgttgcgg gtgaagccca cgccggtgcc gctgttgtcg
      181 tcgcggttgc cgaacaccat ggtctgcacg ttgacggcag tgcccaactc gtggctgatc
      241 cgctcgcgga cgcgataggc gatggcgcgg gcgccgttcc agctccggaa cacggcctcg
      301 accgcaccgc gcaactgctt ggtggggtcc tgggggaacg ccttgccggt gtgggccttg
      361 acgatcgcct tgtaggtctc gcacagcttc ttcaggtcgg cggcgctgat cgcggcatcg
      421 ttggtgacgc cggctgcttc cttgacggct tcgaactcgt gctcgaacgg ctcgcccggc
      481 acgtcgagca cgatgcggcc gtacatcgag atgaaccggc gatagctgtc gtaggcgaag
      541 cgctcgttgc cggtaacggc ggcaagaccc ttgacgctct tgtcgttgag accgaggttg
      601 aggatggtgt ccatcatgcc cggcatcgag aacttggcgc cggagcggac gctgaccagc
      661 agcgggtcgg acgggttgcc aagggtgcgg cccatctgcc tttccagcgc ggcgacctgc
      721 gtcgcgatct cgtcgtcgag gccggtcggc caaccgctgt gcatgtacgc gcggcaggcg
      781 tcggtggaga tggtgaagcc gggcggtacc ggcagcttga gcacgctggt catttcagcc
      841 aggttggcgc ccttgcctcc tagcaggtcc ttgtactcca tgggagggcg gcggtgcttg
      901 tgagagaagg catagacgta ggacacgttg cgcgagtcta tggggcggcg ggcccgcgca
      961 ctgaatgctc ccgtacactc ggcacatggc gaccagggac aacgcagccc acggcggctc
     1021 ccgcggcctg cacgtgttcg gcttcccggt cgacgtgcgc ccggggttcg tgttcttgct
     1081 cgtgctgttc ggggtggtct actccggccg cgggcagggt gagctcggct tgtggctggc
     1141 ggtgttcgtc gccgcgttca cgctggcgca cgagttgggg cacgcgttcg cggcccgcgc
     1201 caccggcgcc aacgcctcga tcgcgctcga cttcctggcc gggtacgcct cgttcgaacc
     1261 ggtgcgaccg ctgaagcgat gggagcgggc cggcatctcg gtggccggcc cggcggtgca
     1321 gatcgcgctc ggcagcatcg tggtcgtcgc cctcggcgcc aacccgttgt cgtacgacag
     1381 catcaacgac tcacccgcca cgttcgcagc gtggtgggcc gggccgatca tcggcctgat
     1441 caacctgatc ccgatcctgc ccctcgacgg aggcaacatc gtgatgtcgg gcctcgacgt
     1501 ggtactgccc ggacgctcgc gcaccgtgat gttgtacttc agtctggccg tgaccatcgg
     1561 cgcgatcgtg tacttcacgc tgcacggctc gctgtcgccg atcttcttct tcttctttcc
     1621 gttgttcagc cagctacaga tgctgcagta ccgccacgcc cacaccgcga tcgacaagca
     1681 cgggtcgtgg cagctgtggg cggcggcatc cgagcgcgaa gcgtggacca tcgccaagcc
     1741 cggcacgttt ccgcccggca tcgtcgcttc gccctacttc cgggcagccc agttggcccg
     1801 ccgcggtcag ctcgacgccg cccgcgatct gctgctgtcc gacttccaat ccgaccgcac
     1861 gccgaactgg atgccaccgg acgcggcgag cccgcgcgag atggccgcgc tgatcgaact
     1921 gctcggcacc ccgctgccaa ccggcaaccc gtacgccgag tacgtgttgg cgacgcagtt
     1981 ggttcgtctc ggcgaatacg agccggccgg gctctacgcg gcctcctcgt acggacgctc
     2041 accgggcacg atgactgcgg tcgtcgtggc ccagtgcgcg gcggcgctcg gcgacgacga
     2101 actggcggtg tcgtggctgc gggccgcctc ggcgatcggc accaactcgg cagggctgct
     2161 cgatgccatc gacgagcgga tcgagttcgc cacgatccgc catcgccccg acgtcgcccg
     2221 actgcgccaa gacgtggtcg cctcggcagc ctgagctcac gaccgggcac actgctcacg
     2281 accgggcaca ctgggggcat gtccccgttc tggtgcccca tcaccctcga tgtcgtcgac
     2341 gtgtccggcg ccgacgccgc caccttcctg cagagccagc tcagccagaa cctgaccggg
     2401 ttgacggtcg gccaggcggt gtacagcttc gtgctgcaac cggccggcaa gctcgacgcg
     2461 ctggtacggg tgcgccgggt c
//
LOCUS       DAORNF010000530         2480 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_31862_length_2480_cov_1.851134, whole genome shotgun sequence.
ACCESSION   DAORNF010000530 DAORNF010000000
VERSION     DAORNF010000530.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31862_length_2480_cov_1.851134"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            226..>811
                     /locus_tag="PLV68_17310"
     CDS             226..>811
                     /locus_tag="PLV68_17310"
                     /inference="COORDINATES: protein motif:HMM:NF014426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HRE03061.1"
                     /translation="MIELRSNSAADTRAIAAAIAGVARAGDLIVLVGEMGAGKTAFAQ
                     GFAKALGVNEPVTSPTFTIVHTYPPVAAGRRARSGRDPLAGLRDSDATSSTANTNTAN
                     TNTVELHHADLYRLDHTTEVADLALDELLDGDGIVLVEWGDVVDLGPHLRVELIANDH
                     DEFERDIEVSTHDTRWNQRWPRLVAAAPSPRRFAG"
     assembly_gap    812..829
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <830..1543
                     /gene="tsaB"
                     /locus_tag="PLV68_17315"
     CDS             <830..1543
                     /gene="tsaB"
                     /locus_tag="PLV68_17315"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HRE03062.1"
                     /translation="IETATTVVGVALVAMQADGDSAVLGSVEIHHGRRHAEVLAPAID
                     FVCEHAQVSLDTIGGVVVDRGPGLFTGMRVGIATASALGFALDVPVVGVSSLDVLAAG
                     GIPPTRASGDDETEDPVVAAVIDARKGEVFFRLYRRGVALGDATTGSIADFNAAVMAR
                     GQRVIAVGDGAWRYRDELAGDVVAAPLAHPSAVVAARVGRAALVAADLDGTRPTVQPL
                     YLRAPDAQINWTQRSSGDR"
     gene            1647..2204
                     /gene="rimI"
                     /locus_tag="PLV68_17320"
     CDS             1647..2204
                     /gene="rimI"
                     /locus_tag="PLV68_17320"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HRE03063.1"
                     /translation="MLSRLLNRPSGPRGLVIEPMRRRDLTAIQVIERQVYPQPWSVNV
                     FANEIEMSRQGQRYYICAHRDSMLVGYAGMMVVLDEAHITNVAVDPQRQRQGIGQRLL
                     AELAWEARRRGCNALTLEVRLSNVAAQAMYQRFGFEPAGIRAKYYENVEDAIVMWCNG
                     IDTPDYARRLRQIVPGCDGDPGQGS"
     gene            2210..>2480
                     /locus_tag="PLV68_17325"
     CDS             2210..>2480
                     /locus_tag="PLV68_17325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03064.1"
                     /translation="MPDKSTIVLGIETSCDETAAAVVMGGHDILSSVVSSSIDDHVPF
                     GGVVPEIASRAHVEQIAVLHPHIGARQGKDHHAEPAPAFRLLPLDR"
BASE COUNT          392 a          819 c          876 g          375 t
ORIGIN      
        1 atccctgttg cggtcggcga cgaagcggtg ctgctgggcc gtcagtcggg cgcgggcggc
       61 cgcggcgagg tgcgcgccga agagtgggcc gagcgactcg gcacgatcgg ttatgagatc
      121 gtgtgcggca tcagcagccg catcgagcgt cgctacaccc gctgaacggc tcacccgctg
      181 agcaggcggg tcgccgcgga cggcgcagat agcctggcgg actgcatgat cgaactgcgc
      241 tcgaactctg ccgcggacac gcgcgccatc gccgcggcga tcgccggggt cgcccgcgcc
      301 ggtgacctga tcgtgctggt gggggagatg ggtgcgggca agaccgcgtt cgcgcagggc
      361 ttcgccaaag cgctgggggt caacgagccg gtgacatcgc cgacgttcac catcgtccac
      421 acgtatccgc cggtcgccgc cggtcgccgc gcccgatccg gccgtgatcc gctggccggg
      481 ttgcgcgaca gcgacgcgac gagcagcacg gcgaacacca acacggccaa caccaacacg
      541 gtcgagctgc atcacgccga cctgtatcgg ttggatcaca cgacggaggt tgccgatctg
      601 gccctggacg agttgctcga cggggatggc atcgtgttgg tcgagtgggg cgatgtcgtc
      661 gatctcgggc cgcacctgcg cgtcgaactg atcgccaacg atcacgacga gttcgaacgc
      721 gacatcgaag tcagcaccca tgacacacgg tggaaccagc gttggccgcg cctggtcgcc
      781 gccgcgccat cgccgcgtcg atttgcaggt annnnnnnnn nnnnnnnnna tcgagacagc
      841 gaccaccgtg gtcggtgtcg cgctggtggc gatgcaggcc gacggcgaca gtgctgtgct
      901 cggttctgtc gagatccacc acggccgacg ccacgccgag gtgctcgccc cggcgatcga
      961 cttcgtgtgc gagcacgccc aggtctccct cgacacgatc ggaggcgtcg tcgtcgaccg
     1021 tgggccggga ctgttcaccg ggatgcgggt gggcattgcc acggcctcgg cgttgggctt
     1081 cgcactcgac gtgccggtgg tgggcgtgtc gtcgttggac gtgctcgccg caggcggcat
     1141 ccccccgacg cgcgcatccg gtgacgacga gaccgaagat cccgtcgtcg cggccgtgat
     1201 cgacgcgcgc aagggcgagg tgttcttccg cctctaccgt cgcggcgtcg cgctcggcga
     1261 tgcgaccacc gggtcgatcg ccgacttcaa cgccgccgtc atggcccgcg gccagcgggt
     1321 gatcgcggtc ggtgacggcg cgtggcggta tcgcgacgag ttggccggag acgtcgttgc
     1381 cgctccgctg gcccacccga gtgcggtggt ggcggcacga gtcggccgcg ccgcgttggt
     1441 ggccgccgac ctggacggca cccgaccgac cgtccaaccc ctctacctcc gcgcccccga
     1501 tgcccagatc aactggaccc agcgcagttc tggggatcga tgagcgtgcg gagcatgcgg
     1561 gcagacgagc gagcgccagt gagccggatg cggatggggc gcggagcgaa gcggagcggg
     1621 ggcgcagccc ccgaggatca atgagcgtgt tgtcgcgact gctcaaccgg ccctcggggc
     1681 cgcgggggtt ggtgatcgag ccgatgcggc ggcgcgacct gaccgcgatc caggtcatcg
     1741 agcgccaggt gtacccgcag ccctggtcgg tcaacgtgtt cgccaacgag atcgagatgt
     1801 cgcgccaggg ccagcgctac tacatctgtg cccaccgcga ctcgatgctg gtcggctatg
     1861 cggggatgat ggtggtgttg gacgaagcgc acatcaccaa cgtcgcggtc gacccgcagc
     1921 gccagcgcca gggcatcggc cagcgactgt tggcggaact ggcgtgggag gcccgccgcc
     1981 gcggatgcaa cgcgctgacg ttggaagtgc gtctgtcgaa cgtggccgcc caggcgatgt
     2041 atcaacgatt cgggttcgag ccagccggca tccgggccaa gtactacgag aacgtcgagg
     2101 atgcgatcgt gatgtggtgc aacggcatcg acacacccga ctacgcccgc cgcctgcgcc
     2161 agatcgtgcc cggctgtgac ggtgacccag gacagggatc gtgatcgcca tgcctgacaa
     2221 gtccacgatc gtgctcggca tcgagacgag ctgcgacgag accgcggcgg cggtggtgat
     2281 gggcggccac gacatcctca gcagcgtcgt cagcagttcg atcgacgacc acgtgccctt
     2341 cggcggtgtc gtgcccgaga tcgccagccg cgcccatgtc gagcagatcg ccgtccttca
     2401 tccgcatata ggagcgcggc aagggaaaga ccatcatgcc gaaccggccc cagccttccg
     2461 ccttttgccg ctcgatcgcc
//
LOCUS       DAORNF010000531         2473 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_31987_length_2473_cov_1.681969, whole genome shotgun sequence.
ACCESSION   DAORNF010000531 DAORNF010000000
VERSION     DAORNF010000531.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31987_length_2473_cov_1.681969"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            144..>827
                     /locus_tag="PLV68_17330"
     CDS             144..>827
                     /locus_tag="PLV68_17330"
                     /inference="COORDINATES: protein motif:HMM:NF022427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2804 family protein"
                     /protein_id="HRE03065.1"
                     /translation="MTTHEHEITAPVDLCLPDSPSLNPDALGWSRVPLHRANLWGEQG
                     RNKRWDYWAVLAGDLTLSIVYSNIDHFGLVDVFWADLATGESGTAATLAEPRAVVLPE
                     RCGTTPLHFDRDGIRVGLVDDAGGTRLQAAWTDADGRSADFDVYVELPPRHESLNVVI
                     PWDDSTFNFTSKHQARPAHGVAHVGGREWVIGDDAWGVLDVGRGRWPHVIHWNWGGGA
                     GRSRATGSDP"
     assembly_gap    828..963
                     /estimated_length=136
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <964..1182
                     /locus_tag="PLV68_17335"
     CDS             <964..1182
                     /locus_tag="PLV68_17335"
                     /inference="COORDINATES: protein motif:HMM:NF022427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2804 family protein"
                     /protein_id="HRE03066.1"
                     /translation="PMAPWTITDPEGQLHAVLTPRYDKHSGADAGEFASETHQVFGTF
                     TGFVVTDDGERVEFDAIQGFAEEARQRW"
     assembly_gap    1298..1402
                     /estimated_length=105
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1563..>2473)
                     /locus_tag="PLV68_17340"
     CDS             complement(1563..>2473)
                     /locus_tag="PLV68_17340"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRE03067.1"
                     /translation="LAKLGASYISADAGGSGSGACSNGEIKDIAIVSPWYAAQPATKE
                     VIDAFEKDAKAAGFNVTKVDTNNDLAAVNGEIENAVAQGADAVVLGMGDPQEFGAGLA
                     ATIAKNVPVIGLDAGVVDGVTANVASDNAFLGEISAQQIIDNIPAGGKVIMLHFDPFE
                     PVRLRDAAAKALFEANGIQIIEYIQGDAADSTGFAKATVQDLLSKYPKGEVDAIWAGW
                     DATALGAFQATQETGRTEVLVTGVDGQDFAVTEVAKGENWIATVRQDWPSIAAKAVEA
                     ISDCSKGTAPTSQLLTVPGLLITKDS"
BASE COUNT          349 a          744 c          761 g          378 t
ORIGIN      
        1 gtaaccgcag acgggcgacg taccgcccgc cgtggtacct atacccaatc agatccggat
       61 gggatattcc aacaattgtt cagcaaggaa cactatttgt ccccgtctct gcgtgaccgg
      121 gcaccacgcg ctagacacgc tgcgtgacca cccacgagca tgagatcacc gcaccagtcg
      181 acctgtgcct gcctgacagc ccctcgctga accctgacgc gctcggctgg tcgcgggtgc
      241 cgctgcaccg cgccaacctg tggggtgagc agggtcgcaa caagcggtgg gactactggg
      301 cggtactcgc tggcgacctc acgctgtcga tcgtgtactc caacatcgac cacttcgggc
      361 tcgtcgacgt gttctgggcc gacctcgcca cgggtgagag cgggacggct gccacgctgg
      421 ccgagccgag agcggtggtg ctgcctgagc ggtgtggaac gaccccactg cacttcgacc
      481 gcgacggcat ccgcgtcgga ctggtcgacg acgcgggtgg cacccggctg caggccgcgt
      541 ggaccgacgc cgacgggcgc tccgccgact tcgacgtgta cgtcgagtta ccgccgcgcc
      601 atgagtcgct caacgtggtg atcccgtggg acgactcgac gttcaacttc acgtcgaaac
      661 atcaggctcg cccggcacat ggcgtcgccc acgtcggcgg gcgggagtgg gtgatcggcg
      721 acgacgcgtg gggcgtgctc gacgtcggcc gcggccgctg gccgcacgtg atccactgga
      781 actggggtgg tggcgcgggg cgcagccgcg ccacgggcag cgatcccnnn nnnnnnnnnn
      841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      961 nnnccgatgg caccgtggac gatcaccgac cccgagggcc aactgcacgc cgtactcacc
     1021 ccgcgctatg acaagcacag cggagccgac gcgggcgagt tcgccagcga gacccatcag
     1081 gtgttcggca ccttcaccgg cttcgtcgtc accgatgacg gcgagcgggt cgagttcgac
     1141 gccattcagg gcttcgccga agaagcccgc caacgctggt gatcgtcccc gcccagcgcc
     1201 gccaaacgcc aagcgccaaa caccaagaaa aggggtagcg gggtcggtgg gaggcgtggc
     1261 agagttgcgg ctaccagaga aaggaggtgg tccaatcnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnccggagtt cgtcccgccg gcacaacggg gagcgacagt
     1441 ctcaacagct tgtggtgagg gcggagccgg atggctccgc cctcctcgcg cagcgggtag
     1501 gtccgccgcg gtcagctgtc tttggtgatc agctgtcgtt gtcgatcagc tgtcgttggt
     1561 gatcagctgt ctttggtgat cagcaggccg gggacggtca gcagctggct ggtcggcgcg
     1621 gtgcccttcg agcaatcgct gatcgcctcg accgccttgg ccgcgatcga cggccagtcc
     1681 tggcgcacgg tggcgatcca gttctcgccc ttggccacct cggtcacggc gaagtcctga
     1741 ccgtcgacac cggtgacgag cacctcggtg cggccggtct cctgggtggc ctggaaggcg
     1801 ccgagcgcgg tggcgtccca cccggcccag atcgcgtcga cctcgccctt ggggtacttc
     1861 gacagcaggt cctgcaccgt cgccttggcg aagccggtcg agtcggctgc gtcgccctgg
     1921 atgtactcga tgatctggat gccgttggcc tcgaacagcg ccttggccgc cgcgtcgcgg
     1981 aggcggaccg gctcgaaggg atcgaagtgg agcatgatca ccttgccgcc cgcggggatg
     2041 ttgtcgatga tctgctgggc gctgatctcg ccgaggaagg cgttgtcgga cgcgacgttg
     2101 gctgtgacac cgtcgaccac accggcgtcg agcccgatga ccggcacgtt cttggcgatg
     2161 gtcgccgcca ggcctgcgcc gaactcctgc gggtcaccca tgcccaacac cacggcgtca
     2221 gccccctggg cgacggcgtt ctcgatctcg ccgttgaccg cggcgaggtc gttgttggtg
     2281 tcgaccttgg tgacgttgaa cccggccgcc ttggcgtcct tctcgaacgc gtcgatcact
     2341 tccttggtgg cgggctgggc cgcataccac ggcgagacga tggcgatgtc cttgatctcg
     2401 ccgttgctgc atgcgccgga accggagccg ccagcgtcgg cgctgatgta gctggcgccc
     2461 agtttggcca act
//
LOCUS       DAORNF010000532         2470 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_32044_length_2470_cov_1.464596, whole genome shotgun sequence.
ACCESSION   DAORNF010000532 DAORNF010000000
VERSION     DAORNF010000532.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32044_length_2470_cov_1.464596"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>304
                     /locus_tag="PLV68_17345"
     CDS             <1..>304
                     /locus_tag="PLV68_17345"
                     /inference="COORDINATES: protein motif:HMM:NF019633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-interacting protein"
                     /protein_id="HRE03068.1"
                     /translation="QPPGAYYTVRSWDPVVGEMTCDFVLHGDEGLASAWATRARPGDK
                     VALWGPRTAYAPPADTEWYLLVGDETGQPGIGAIVDSLPADAVVHAVIEVSDDGERQ"
     assembly_gap    305..387
                     /estimated_length=83
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(600..1286)
                     /locus_tag="PLV68_17350"
     CDS             complement(600..1286)
                     /locus_tag="PLV68_17350"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRE03069.1"
                     /translation="MTTSANRRAEMRRKQTQHQGEASRSMLWWIVGGVLLAVILSAVV
                     AIATNKDDEVVEIPANYEQNRPVAVSGTSLPGIPQGGGPDPALGMTAPIVTGQSFDGS
                     PVTTAGNGPRMIVVVAHWCPHCRNEVPRLVQWQQSGQMPAGLDVVFVSTSVQSNAPNY
                     PPSRWLEDVEHVTWPTLADDVAQTAAAALGTSGFPYFLVVDANGTVVLRDSGEQDTAS
                     LTARIEAALG"
     gene            complement(1286..>1560)
                     /locus_tag="PLV68_17355"
     CDS             complement(1286..>1560)
                     /locus_tag="PLV68_17355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03070.1"
                     /translation="VALAAIGTVISLYHWLLERFPSLDTGACSSAVPCEFVWFEEFGF
                     VTLPFMALTGFVTIIAWVTIPEPTPDAAPNHTSPDFTHPDSTPEET"
     assembly_gap    1561..1712
                     /estimated_length=152
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2090..2202
                     /estimated_length=113
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2203..>2470)
                     /locus_tag="PLV68_17360"
     CDS             complement(<2203..>2470)
                     /locus_tag="PLV68_17360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03071.1"
                     /translation="PETNPDDDTATTTTVATSSLDVYLVRDDRVTRVSRVAAATDIAT
                     VLNLLAAGPTEREVERGLRTALVPELVSLAEVAEVADAVATIDLT"
BASE COUNT          314 a          693 c          778 g          337 t
ORIGIN      
        1 cagccgccgg gggcctatta caccgtgcgc tcctgggacc ccgtggtggg cgagatgacg
       61 tgtgacttcg tgctccacgg cgatgagggc cttgcgtcgg catgggcgac gcgggctcgg
      121 cccggcgaca aggtggcgct gtggggtccg cgcacggcct atgcgcctcc ggccgacacg
      181 gagtggtacc tgctcgtcgg ggacgagacc ggccagccgg gcatcggcgc gatcgtcgac
      241 tcgttaccgg cggacgcggt ggtgcacgcc gtcatcgaag tgtccgacga tggcgagcgc
      301 cagcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnggt cgatgccttg gccgggcggc cgggcctacg
      421 cctggggtgg cggcgagagc cgtgccgtga ccgcgattcg caaatatctg cgcaccgagg
      481 tcggtctggc tcgcgaggcg gtgtcgatga cggggtactg gcgccatgcc gacacgccgg
      541 tcgaggtgga ctgaccgtga catcgaccgg cgaggatcga tccgcgatgt cgggggtggt
      601 cagcccagcg cggcttcgat gcgagcggtc aggctcgctg tgtcctgctc gccgctgtcg
      661 cgcagcacca ccgtgccgtt ggcgtcgacc acgaggaagt acgggaaccc gctcgtgccc
      721 aacgctgcgg ccgcggtctg ggcgacgtcg tcagccaggg tcggccaggt gacgtgctcg
      781 acgtcctcca accaccgcga cggcgggtag ttcggcgcgt tcgactgcac cgacgtcgac
      841 acgaacacga cgtcgagccc ggctggcatc tgtccgctct gctgccattg caccaaccga
      901 ggtacctcat tgcggcaatg tgggcaccag tgggcgacca ccacgatcat tcgcggtccg
      961 ttgcctgccg tggtcacggg cgagccgtcg aaggactggc cggtgacgat cggtgcggtc
     1021 atgcccagcg ccgggtcggg gccaccgcct tgggggatac cgggcagcga cgtgccgctg
     1081 acggcgaccg gcctgttctg ctcgtagttg gctggaatct cgaccacctc gtcgtccttg
     1141 ttcgtcgcga tcgcgaccac ggccgacagg atcactgcca ggagcacacc gccgacgatc
     1201 caccacagca tggagcggct ggcctcccct tggtgctggg tctgcttgcg gcgcatctcg
     1261 gcgcggcgat tggcggaggt ggtcatcagg tttcctcggg ggtggagtct gggtgggtga
     1321 agtctgggct ggtgtggttg ggggccgcgt caggagtcgg ctcggggatc gtgacccagg
     1381 cgatgatggt gacgaagccg gtcagcgcca tgaacggcag ggtgacgaag ccgaactcct
     1441 cgaaccacac gaactcgcac ggcaccgcag acgagcaggc accggtgtcg agcgacggga
     1501 agcgttcgag caaccagtgg tagagcgaga tcaccgtgcc gatcgcggcc aacgccacgg
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngccgcgaa cggcctggcc atcggcacga
     1741 ggtcgtcctg caactggcgt gcccccggcg agcggcgggc ggcgagcctg atgccgatca
     1801 cggcaacggt cgcggccaac gcccccaacg tgaggagcgc gaagaacaac tgcgcggtct
     1861 gtggattcat ggatggctac ctgtcgacca gtcgctcgcc ggaggcgatc ggttcccgcg
     1921 ctggtacgaa catgcccaaa tcgtcgtcgg ttctcggcga gtcgacaacc tccattggtc
     1981 cctgattcac gaaccggctg gggcgaggtc ggcgtagtcg agcggcgtga ctggcgtctc
     2041 ggtggtcgac ccgtcgccgc ggggcaccgc cgccggctcg ccccccaccn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnggtcaggt cgatggtggc
     2221 gaccgcatcg gcgacctcgg ccacttcggc cagcgacacc agctcgggca ccagcgctgt
     2281 gcgcaggccg cgctcgacct cgcgctcggt cggacctgcg gccagcaggt tcaacaccgt
     2341 ggcgatgtcg gtggccgcgg caacgcgcga cacccgcgtc actcggtcgt cgcgcacgag
     2401 gtagacgtcg agcgaactgg tcgccacggt cgtggtggtg gccgtgtcgt cgtccgggtt
     2461 ggtctctgga
//
LOCUS       DAORNF010000533         2457 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_32236_length_2457_cov_1.863031, whole genome shotgun sequence.
ACCESSION   DAORNF010000533 DAORNF010000000
VERSION     DAORNF010000533.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32236_length_2457_cov_1.863031"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    242..359
                     /estimated_length=118
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <360..824
                     /locus_tag="PLV68_17365"
     CDS             <360..824
                     /locus_tag="PLV68_17365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03072.1"
                     /translation="DKGKCDKDALWTPNSEKAPTLRDYIAGVGANFGRVRIIKLEPQD
                     HEAAFRSIHRDDNNRFNPDSDGWVVRTWVELTDNPDSYMLLMDNDADGLPDPATEVRV
                     PLHKGARFLVDSQRLWHVVVHNGTSPRYALITSFETSPVLETWVDANAPTPV"
     gene            896..>2457
                     /locus_tag="PLV68_17370"
     CDS             896..>2457
                     /locus_tag="PLV68_17370"
                     /inference="COORDINATES: protein motif:HMM:NF012935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HRE03073.1"
                     /translation="MTFDYIIVGAGAAGCVLANRLSADPGVRVLLVENGGRDRNPLLS
                     IPKGFSFTLRGDRYTYKYETQPVGPARQSESWLRGKVSGGSTSINGMMYVRGHQRDYD
                     DIVGRGNPGGGWEQMLAVFRAMEDHALGASPMRGVGGPLGVSIAVQHDQVCDAIVEAA
                     AALGLRDTDDFNAADDERIGFTPSTIRRGVRVSAARAFLRPIAKRPNLTVLHRTRVGE
                     LRFDGNRVVGVRTRSGGGVRDYTARREVIVSAGTIETVLLLERSGIGRGDVLRAAGID
                     VRADSPNLGERVIEQRGVSLQMKLKGKLGLTHTMNSVPKQIVQGARYLATRRGPLSTG
                     GYDLTCAFKSAPGLDRPDVQGVLMPLALDTGTDNIQLADYAGATFVGWQIRPTTTSSI
                     HIGGAQPEDAPIISPRYVETDVDRAATGPMLTWARELFAQQPLAALVDSEVLPGPSVS
                     TPAEVVTHAQNVSVGLYHAVGSAAMGPDDDDVVDDHLRVRGVQGLRVVDASVFAAQPA
                     GNTAAPTMAVAWR"
BASE COUNT          372 a          790 c          815 g          362 t
ORIGIN      
        1 atgcgatcgt cggcgtgggt gtcgtgatga tggtccgcac agcactgaga gcactccgtt
       61 tcccttgagg gcgtcccgac cggttcaggg tcacatggcc ctaccggagg ggtgagagtt
      121 ctctaggctt ggggctatgg gtttttggcc gcatgcaggg cactggtccg tcgccatccc
      181 gctgcacaca ccgcacacgc gtctggccgc actggccgag cacgggtggg tgcaactgtc
      241 cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng
      361 acaagggcaa gtgcgacaag gacgcgctgt ggacgcccaa ctccgagaag gcccccacgc
      421 tgcgtgacta catcgccggc gtcggcgcca acttcggccg ggtgcggatc atcaaactgg
      481 aaccgcaaga tcacgaggcc gcgttccgca gcatccaccg cgacgacaac aaccggttca
      541 accccgacag cgacggctgg gtggtgcgca cgtgggtcga actcaccgac aaccccgaca
      601 gctacatgct gttgatggac aacgacgccg acggcctgcc cgacccggcg accgaggtgc
      661 gagtgccgct gcacaagggt gcccggttcc tggtcgactc ccagcgcctg tggcacgttg
      721 tggtccacaa cggcaccagc ccccgctacg cgctgatcac cagtttcgag acctcgccag
      781 ttctcgaaac ctgggtcgac gccaacgccc cgacgcccgt ctagggcgac cgtcgtcccg
      841 gtggagtcca ctgttccccc gtgcgctggt cggatcagac agatcatcaa tctgcatgac
      901 gtttgactac atcatcgtgg gggccggggc ggcgggatgt gtgctggcca atcggctcag
      961 cgcggatccc ggtgtgcggg tgttgctcgt cgaaaacggt gggcgcgacc gcaacccgct
     1021 gctgtccatc cccaagggct tctccttcac cctgcgcggc gaccgctaca catacaagta
     1081 cgagacgcag ccggtcggcc cggcgcgcca gtccgagtcg tggctgcgcg gcaaggtgag
     1141 cggcgggtcg acgtcgatca acgggatgat gtacgtccgt ggccaccagc gtgactacga
     1201 cgacatcgtg gggcggggca acccaggggg gggttgggag cagatgctgg cggtgttccg
     1261 ggcgatggaa gatcacgcgc tcggtgcctc accgatgcgt ggcgtcggcg gaccgctcgg
     1321 cgtgtcgatc gcggtgcagc acgatcaggt ctgcgacgcg atcgtcgagg ccgcggcagc
     1381 gctcggcctg cgcgacaccg acgacttcaa cgccgccgac gacgagcgca tcggattcac
     1441 cccgtccacg atcaggcggg gcgtgcgcgt cagcgcggcg agggcgtttc tccgccccat
     1501 cgccaagcgg cccaacctga ccgtgctgca ccgcacccgg gtgggtgagt tgcggttcga
     1561 cggcaaccgt gtggtgggtg tgcgcacccg gtcgggtggt ggcgtgcgtg actacacggc
     1621 gcgccgcgag gtgatcgttt cggcgggcac gatcgagacg gtgctgctgt tggagcgctc
     1681 cgggatcggc cgcggcgatg tgctgcgtgc tgccgggatc gacgtccgcg ccgacagccc
     1741 gaacctgggc gagcgcgtca tcgaacagcg cggcgtctcg ctgcagatga agctgaaggg
     1801 caagctcggt ctgacccaca cgatgaactc ggtgccgaag cagatcgtgc agggggcgcg
     1861 ctacctggcg acccggcgcg gtcccctgtc gaccggcggt tacgacctga cgtgcgcgtt
     1921 caagtcggcc cccggcctcg accgccccga tgtgcagggg gtgctgatgc cccttgcact
     1981 cgacaccggc accgacaaca tccaactcgc cgactacgcc ggggcgacgt tcgtgggctg
     2041 gcagatccgg ccgacgacga ccagctcgat ccacatcggc ggcgcgcagc ccgaggatgc
     2101 accgatcatc tcgccccgct atgtcgagac cgacgtcgac cgggccgcga ccgggccgat
     2161 gctcacatgg gcgcgcgaac tgttcgcgca gcagccactt gccgcgctgg tcgacagcga
     2221 ggtgcttccc ggcccgtccg tctcgacgcc agccgaggtg gtcacccacg cccagaacgt
     2281 ctcggtcggg ctgtatcacg cggtcgggtc ggcggcgatg gggcccgacg acgacgacgt
     2341 ggtggacgac cacctgcggg tgcggggcgt acagggcttg cgcgtggtcg acgcgtcggt
     2401 gttcgcggcc caaccggccg gcaacaccgc ggccccgacg atggccgtcg cctggcg
//
LOCUS       DAORNF010000534         2455 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_32266_length_2455_cov_1.845417, whole genome shotgun sequence.
ACCESSION   DAORNF010000534 DAORNF010000000
VERSION     DAORNF010000534.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32266_length_2455_cov_1.845417"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2455)
                     /locus_tag="PLV68_17375"
     CDS             complement(<1..>2455)
                     /locus_tag="PLV68_17375"
                     /inference="COORDINATES: protein motif:HMM:NF017545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MYXO-CTERM sorting domain-containing protein"
                     /protein_id="HRE03074.1"
                     /translation="STTCELEGEYSCARAVSFNNLNVQNFPGGNPLANWIIASGVEGV
                     QTNNTSLPTVALFGENAQFGTYAVRMRVETDQDDDVIGLIMGFSPGDQTNNAANYLVV
                     DWKQGLQEGVPAGLRSAHVRGVPFVNANSWQHGIPARRCDNTATSCVTQLANGRQLGT
                     TGWADNTSYTAYITYRPDRLELLVNNRLELLLRPTDFPGQFPGNVFPPGQMGFYLHSQ
                     DQVRYTNLAPNGASACNLTTLNPATINVALGTPSVNVNTAALLTDMGDALAPTTVTIL
                     RPVTGGTATVGANGSVTFTPTNPNVPGRYQIEVYACDNDPVIPDCDSAIITINYGIDT
                     DGDTVTDDLDLDDDNDGIPDAVERGKDTDGDGVPDDLDLDSDNDGLPDVVEAGHGQPD
                     ANGDGLIDCAGGFGVNGLCNAVETAPDSGVGRTPPVDTDGDGIPDFRDLDSDDDGLAD
                     RQENGTRCMDRPINNVCDGNDADGDGSPSSADGSPGFGVGGYPMPPDTDGDGTPDYRD
                     TDSDGDGIPDVVENDRGAADTNDDGRVDGPDRDGDGLRDAVDDSDLDGIPDSEDTGPP
                     SFGGGLPVLDTDDDDLPDYLDPDSDDDGLPDADESGDDPHNPIDSDNDGTPDFQDPDA
                     DNDMIADGSDNCPRVGNPDQENQDRDGLGDSCDPDANGDGFDDSLGVSGGGCSTGGTA
                     GTSILLVALAALFLRRRKTALVVALVALSSAGQAQAQVSSSYSAERFLLTGHRDGILG
                     LEWAEVKGHLTVDLGVWLGYANDPVNVYRMSDGDRVGSLVSNRFGGDLVAAVQLKDRF
                     ELGIAAPIIVSQSQDLDSLT"
BASE COUNT          353 a          805 c          864 g          433 t
ORIGIN      
        1 ggtcagcgaa tcgaggtcct gcgattgcga cacgatgatc ggcgcggcga tgcccagctc
       61 gaagcgatcc ttgagctgca ccgcggctac caggtcgcca ccgaagcggt tggacaccag
      121 cgagcccacc cgatcgccgt cggacatccg gtacacgttc accgggtcgt tggcgtagcc
      181 gagccacacg ccgagatcca cggtgaggtg gcctttgacc tcggcccact ccaggccgag
      241 gatgccatcg cggtgaccgg tgagcaggaa gcgctccgcc gagtacgagc tcgacacctg
      301 cgcttgcgcc tgccccgcgc tggagagcgc caccagcgcc accactagcg ccgtcttgcg
      361 gcggcgcaaa aacagcgccg ccagcgccac cagcaggatg ctggtgcccg cggtgccgcc
      421 cgtcgagcag ccgccaccgg acacgcccag gctgtcatca aagccatcgc cgttggcgtc
      481 cggatcgcag gaatcgccca gcccgtcgcg gtcctggttc tcctgatcgg ggttgcccac
      541 gcgcgggcag ttgtccgagc cgtcggcgat catgtcgttg tcggcatccg gatcctggaa
      601 gtcgggcgtg ccgtcgttgt cgctgtcgat cgggttgtgc ggatcgtcgc cggactcgtc
      661 ggcgtcgggc aggccgtcgt cgtcgctgtc gggatcgagg tagtcaggta ggtcatcgtc
      721 gtccgtgtcg agcaccggca ggccgccacc gaagctcggc gggccggtgt cctcggagtc
      781 ggggatgcca tcgaggtcgc tgtcgtcgac agcgtcgcgc aggccgtcgc cgtcgcggtc
      841 cgggccgtcg acccggccgt cgtcgttggt gtcggcggcg ccgcggtcgt tctccaccac
      901 gtcggggatg ccatcgccat cgctgtcggt gtcgcggtag tcgggcgtgc catcgccgtc
      961 ggtatcgggc ggcatcgggt agccgcccac gccgaagcca ggcgagccat cggccgagct
     1021 gggcgagcca tcgccgtcgg cgtcgttgcc atcacacacg ttgttgatgg gacgatccat
     1081 gcagcgcgtg ccgttctcct gccgatcggc caggccatcg tcatcgctgt ccagatcgcg
     1141 gaagtcgggg atgccatcgc catcggtgtc gaccggcggg gtgcgcccca cgccgctatc
     1201 cggcgcggtc tccaccgcgt tgcataggcc gttgacgcca aagccgccgg cgcagtcgat
     1261 gaggccatcg ccgttggcgt cgggctggcc gtggccggcc tcgaccacgt cgggcaggcc
     1321 gtcgttgtcg ctgtcgaggt cgagatcgtc cggcacgccg tcgccatcgg tgtccttgcc
     1381 gcgctccacc gcgtcgggga tgccatcgtt gtcgtcatcg agatcgaggt cgtcggtgac
     1441 ggtatcgcca tcggtgtcga tgccgtagtt gatggtgatg atcgccgagt cgcagtccgg
     1501 gatcaccgga tcgttgtcgc aggcgtacac ctcgatctgg tagcgacccg gcacgttggg
     1561 gttggtcggc gtgaaggtga cagagccgtt ggcgccgacg gtggcagtgc cgccggtcac
     1621 cggtcgcaag atcgtcaccg tggtcggcgc cagcgcatcg cccatgtcgg tgagcagcgc
     1681 cgccgtgttg acgttcacgc tcggcgtacc gagcgccacg ttgatggtgg ccgggttgag
     1741 cgtggtgagg ttacacgccg aggcgccgtt gggcgcgagg ttggtgtagc gcacctgatc
     1801 ctgcgagtgc aggtagaagc ccatctggcc cggcggaaac acgttgccgg gaaactggcc
     1861 ggggaagtcg gtggggcgaa gcagcagctc gaggcggttg ttcaccagca gctcgagccg
     1921 gtccggccgg tacgtgatgt acgcggtgta cgaggtgttg tcggcccagc ccgtggtgcc
     1981 gagctgacgc ccgttggcga gctgggtcac acacgaggtg gcggtgttgt cacagcgtcg
     2041 agcaggaatg ccgtgctgcc acgagttggc gttgacgaaa ggcacgccgc gcacgtgcgc
     2101 cgagcgcagg ccggctggca ccccctcttg cagcccttgc ttccaatcga ccaccaggta
     2161 gttcgcggcg ttgttggtct gatcccccgg agagaacccc atgatcaggc cgatcacgtc
     2221 atcgtcctga tcggtctcca cccgcatgcg caccgcgtag gtgccgaact gcgcgttctc
     2281 accgaacagc gccaccgtcg gcagcgaggt gttgttggtc tgcacaccct cgacaccgct
     2341 ggcgatgatc cagttggcca gcgggttgcc gccagggaag ttctgaacgt tgaggttgtt
     2401 gaagctcacc gcccgcgcac acgagtactc gccctcgagc tcgcaggtgg tgctg
//
LOCUS       DAORNF010000535         2453 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_32307_length_2453_cov_1.876564, whole genome shotgun sequence.
ACCESSION   DAORNF010000535 DAORNF010000000
VERSION     DAORNF010000535.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2453
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32307_length_2453_cov_1.876564"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..794
                     /locus_tag="PLV68_17380"
     CDS             <1..794
                     /locus_tag="PLV68_17380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HRE03075.1"
                     /translation="IGLYGKPTIVNNVETLSNLPWLAVNGPEAYLAIGTPTSPGTRMV
                     AVSGHVKRPGVFEIVNGTTTFRDLLYGDEFCQGIRDDHKLKAFVPGGGSAPWFLPDQL
                     DLPFEGRLVGAAGSMLGSGEIIVMDHTTDIPKAALTLTRFYAHESCGKCSPCREGGTW
                     LMRILQRVVEGNATSRDLDQLYQVGEIICPGAFPHAASAELGLDAVPFPYKMTTICFV
                     GPSAYAPVHSALTLFRPEFEAKLIKRVFIPVSAAAADAPVPAGSH"
     gene            794..>2098
                     /locus_tag="PLV68_17385"
     CDS             794..>2098
                     /locus_tag="PLV68_17385"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HRE03076.1"
                     /translation="MSNTATSSDDAPDATETPEVAPDPNAVQITLNGRPVVARKGELI
                     IKAAERAGEYIPRFCYHERMDSVGMCRQCIVEVDTGRGPQLMVSCMFPVAPDMQVITD
                     SPMVKGVQEGVLEMLLAQHPLDCPVCDKGGECPLQDQAYSHGPGESRYVEEKRHYAKP
                     IPISDLVLLDRERCVLCDRCTRFADEVAGDALIHFINRGNATQVNTFPDEPFASYFSG
                     NTVQICPVGALTAKPYRFKARPWDLDEVESTCMGCSVGCHNALQSTRDELVRIQGVDS
                     EAVNWGWLCDRGRFSFETVGNGERLGTPLVLTEQGHVPTSWNSALGSATQLLRDAVAA
                     GGAGSVAVLGGARLTNEDAFAWARLAHDVIGTPNVSAQLGDGLPVGVLGLPRATIAQA
                     ANAKTIILLGPDLKEELPVLYLRLRDAAVKKRSRIIEFAPKES"
     assembly_gap    2099..2149
                     /estimated_length=51
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2150..>2453
                     /locus_tag="PLV68_17390"
     CDS             <2150..>2453
                     /locus_tag="PLV68_17390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03077.1"
                     /translation="EALRQPDIAAQIATGDVVIVAGRANLAESSAATTAALLALTDAL
                     PQATVLPALRRGNVVGAIQVGMRPGRGGKDSRAILEAAADGKIECLVLVGADPLSDV"
BASE COUNT          388 a          865 c          778 g          371 t
ORIGIN      
        1 ccatcggcct gtacggcaag ccgacgatcg tcaacaacgt cgagactctg tcgaacctgc
       61 cgtggcttgc cgtcaacggc cccgaggcct acctggcgat cggcacgccc acctctcccg
      121 gcacgcgcat ggtcgcggtc tccggccacg tcaagcggcc cggcgtgttc gagatcgtca
      181 acggcaccac cacgttccgc gacctgctct acggcgacga gttctgccag ggcattcgcg
      241 acgaccacaa actcaaggcg ttcgtgcccg gtggcggttc ggcaccgtgg ttcctgcccg
      301 accagctcga tctgcccttc gaagggcgtc tggtcggtgc ggccggatcg atgctcggct
      361 ccggggagat catcgtcatg gatcacacca ccgacatccc caaggcggcg ttgaccctca
      421 cccgcttcta cgcccacgaa tcgtgcggca agtgcagccc gtgccgtgaa ggtggtacgt
      481 ggctgatgcg catcctccag cgcgtcgtcg aaggcaacgc caccagccgc gatctcgacc
      541 agctctacca ggtgggcgag atcatctgcc ccggtgcctt cccgcacgcg gccagtgccg
      601 agctcggtct cgacgcggtg ccgttcccgt acaagatgac cacgatctgt ttcgtcgggc
      661 cgtcggcgta cgcgccggtg cactcggcgc tgacgttgtt ccgcccggag ttcgaagcca
      721 agctcatcaa gcgggtgttc atcccggtgt cggccgcggc cgccgatgcc ccagtccccg
      781 cgggtagcca ctgatgtcga acaccgccac ctcatccgac gacgcaccgg acgcgaccga
      841 gacacccgag gtcgcgcccg atcccaacgc ggtgcagatc acactcaacg gccgtcccgt
      901 cgtggcccgc aagggtgagc tgatcatcaa ggccgccgag cgggcggggg agtacatccc
      961 acgcttctgc taccacgagc ggatggacag cgtcggcatg tgccgccagt gcatcgtcga
     1021 ggtcgacacc ggccgcggtc cgcagttgat ggtgtcgtgc atgttcccgg tcgcgcccga
     1081 catgcaggtg atcaccgact cgccgatggt caagggcgtg caagagggcg tgctcgaaat
     1141 gctgctcgcc cagcacccgc tcgactgccc cgtgtgtgac aagggcggcg agtgcccgct
     1201 gcaagaccag gcctacagcc acgggcccgg cgagagccgc tacgtcgaag agaagcgcca
     1261 ctacgccaag ccgatcccga tcagcgatct ggtcctgctc gaccgtgagc gctgcgtgct
     1321 gtgcgatcgc tgcacccgct ttgccgacga ggtcgccggc gacgcgctga tccacttcat
     1381 caatcgcggc aacgccaccc aggtcaacac gttccccgac gagcccttcg ccagctactt
     1441 cagcggcaac acggtgcaga tctgcccggt cggcgcgctg accgccaagc cgtaccgatt
     1501 caaggcccgg ccgtgggatc tcgacgaagt cgagagcacg tgcatgggct gttcggtcgg
     1561 ttgccacaac gccttgcagt ccacccgcga cgagctcgtg cgcatccagg gtgtcgactc
     1621 cgaagcggtc aactggggtt ggttgtgcga ccgcggccgg ttcagcttcg agacggtcgg
     1681 caacggcgag cggctcggca caccgctggt gctcaccgaa caagggcacg tgcccacgtc
     1741 gtggaactcg gccctcggtt cggcgacgca actgttgcgc gacgcggtgg ccgccggcgg
     1801 tgctggcagt gtggccgtgc tcggcggcgc ccggctcacc aacgaggacg cgttcgcatg
     1861 ggcccgtctc gcccacgacg tgatcggcac gcccaacgtg tcggcccagc tcggcgacgg
     1921 cctgcccgtc ggagtgctcg gcctgccgcg ggccacgatc gcccaggcgg ccaacgccaa
     1981 gacgatcatc ctgctcgggc ccgatctcaa agaagagctg ccggtgctgt acttgcgcct
     2041 gcgtgatgcc gcggtcaaaa agcgcagccg catcatcgag ttcgcgccca aggaatcgnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc gaggcgctcc
     2161 gccagcccga catcgcggcc cagatcgcga cgggtgacgt ggtcatcgtg gccgggcgag
     2221 ccaacctggc cgagtcttcg gcggccacca ccgcggcact gctggcgctc accgatgccc
     2281 tcccgcaggc caccgtgctg cccgcgctgc gccgcggcaa cgtcgtcggt gcaatccagg
     2341 tcggcatgcg cccggggcgc ggcggcaagg acagccgggc gatcctcgag gccgctgccg
     2401 acggcaagat cgaatgcctc gtgctggtcg gcgccgaccc gctcagcgac gtg
//
LOCUS       DAORNF010000536         2444 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_32446_length_2444_cov_1.971118, whole genome shotgun sequence.
ACCESSION   DAORNF010000536 DAORNF010000000
VERSION     DAORNF010000536.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32446_length_2444_cov_1.971118"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    211..229
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <230..647
                     /locus_tag="PLV68_17395"
     CDS             <230..647
                     /locus_tag="PLV68_17395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03078.1"
                     /translation="AHMEAEFAADIDTAMHTLVAEPVFRFWCGYRDPDGPAILARPVI
                     RANYERVFATGFPMIEIVPERFLVSDDGIVLEGEQRSLLGGRQLLDQGITAEAMEQYY
                     VLCRFALLIEFADDLMVGEDHYWPLPHTVLKIVDDR"
     gene            644..1186
                     /locus_tag="PLV68_17400"
     CDS             644..1186
                     /locus_tag="PLV68_17400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03079.1"
                     /translation="MTSSSTSPSTRDRLLPWIGSLESRGAALTDDLRRPVTTLAGALA
                     AVHDRDADRLLATMTDDVLIRIWGWSTGGQWSYRGDEARAATAAWLATIGAGAVGVRV
                     DIDRFFPGDGFLGFDGTWSRLTSGAGLAESTSRFDPGAEYLVSRRFAFFGTFTRTGIS
                     VVDLYWGIHPQIGLSDRSPR"
     gene            complement(1195..1629)
                     /locus_tag="PLV68_17405"
     CDS             complement(1195..1629)
                     /locus_tag="PLV68_17405"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRE03080.1"
                     /translation="MAQISGVMDWREVMKTPPDHVMEGHYNYWFVFEQDDVAIAMFEM
                     MGPLPPHRHPATHWATVLEGEAECVIGDQYFVAKPWDTIRVDIGVPHVVVPRIGQRWV
                     GFELTMPRDIINEVDYLTSGPDFERIDAIEREHGMWRFNPNG"
     gene            complement(1746..2207)
                     /locus_tag="PLV68_17410"
     CDS             complement(1746..2207)
                     /locus_tag="PLV68_17410"
                     /inference="COORDINATES: protein motif:HMM:NF013821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin kinase"
                     /protein_id="HRE03081.1"
                     /translation="MDPSKPQFVSGVLPSPVTAMVELGDQRGRQLGFPTANLHRADCN
                     EQMRGVYAAIACTADGVWWPAAVNVGRRPTFGGDVRTAIEVHLIGFAGDIYDEMLTVR
                     FLQRLRDEVRFESIEALIFATTSMPPRLQSLQRGCSSFDLPLWARRVAGAY"
BASE COUNT          443 a          820 c          746 g          416 t
ORIGIN      
        1 ctccacgtca cgctcagggc agacgagatc cagttcatcg ggcaagcgat cgaggctgcc
       61 ggcacaggcg tcggccccgc ctacccgccg cgcgagacga gtgcggccaa tggttgacac
      121 catcgctcgc tcgcacgccg tggttcggcc cggcaacatg ctcgacgcgc tgcgaaacaa
      181 gatcgcagcg accacgaacc cccgtcacgt nnnnnnnnnn nnnnnnnnnc gcccacatgg
      241 aagccgagtt cgcagccgac atcgacacgg cgatgcacac cttggtcgcg gagccggtct
      301 tccgcttctg gtgcggatac cgcgacccgg acggtccagc catcctcgcc cggcccgtca
      361 tccgcgccaa ctacgagcgc gtcttcgcca ccggtttccc gatgatcgag atcgttccag
      421 agcggtttct cgtcagcgac gacgggatcg tgctcgaggg tgagcagcgt tcgctcctcg
      481 gtgggcgcca gctgctcgac caagggatca ccgccgaggc gatggagcag tactacgtac
      541 tctgccgctt cgcattgctg atcgaattcg ccgacgacct gatggtcggc gaggatcact
      601 actggcccct gccgcacacg gtcctgaaga tcgtggacga tcgatgacat ccagcagcac
      661 gtccccttcg acgcgcgacc gcctgctgcc gtggatcggg tcgctggagt ctcgcggcgc
      721 agcactgacc gacgatctcc gcagaccggt gacgaccctc gccggcgctc tcgccgcggt
      781 gcacgatcgc gacgcagacc ggttgctcgc gacgatgacc gacgacgtgt tgatccgcat
      841 ctggggatgg agtacgggcg gccagtggtc gtaccgcggc gacgaggcgc gtgcagccac
      901 cgcggcgtgg ctggcaacga tcggtgcggg cgcggtcggg gtgcgcgtcg acatcgaccg
      961 gttctttccg ggcgatggct tcctcgggtt cgacggcacc tggtcgaggc tgaccagcgg
     1021 cgccggcctc gccgagtcaa cgtccaggtt cgaccccggc gccgagtatc tcgtgtcccg
     1081 tcgctttgcc ttcttcggca cgttcactcg cacgggcatc agtgtcgtcg acctgtactg
     1141 gggcattcac ccgcagatcg ggctcagcga tcgttcacca cgctgagcga gcggtcaacc
     1201 gttcgggttg aaccgccaca tgccgtgctc gcgctcgatc gcgtcgatgc gctcgaagtc
     1261 cgggccggaa gtgaggtagt ccacctcgtt gatgatgtca cgaggcatgg tcaactcgaa
     1321 cccgacccaa cgctggccga tacggggaac aaccacgtgc ggcacgccga tgtcgacgcg
     1381 gatcgtgtcc cacggcttgg cgacgaagta ctggtcgccg atcacgcact cggcttcgcc
     1441 ttcgagcacc gtcgcccagt gggtcgccgg atggcgatga ggcggaagcg ggcccatcat
     1501 ctcgaacatc gcgatggcga cgtcgtcctg ctcgaagacg aaccagtagt tgtagtgacc
     1561 ttccatcacg tggtccggcg gggtcttcat gacctcccgc cagtccatca ctccagagat
     1621 ctgtgccaag tcgacatgaa tcatgtcaag cccccatctg cgcttgatcg agtcggtgag
     1681 ccgcatcctg tgggtcgcag caaccaaaca cctggtagat agtatgcacc gacggcgctg
     1741 gggcgtcaat acgcacccgc cacacgccta gcccacagcg gaagatcgaa gctcgagcaa
     1801 ccccgctgca acgactgcag acgcggcggc atcgacgtcg tcgcgaagat gagcgcctcg
     1861 atcgactcga agcggacctc gtcacgcaga cgctgcagaa atcgcacggt gagcatctcg
     1921 tcgtagatgt cgcccgcgaa gccgatcaga tgaacctcga tcgcggtcct cacatcaccg
     1981 ccgaacgtcg ggcgacggcc gacgttgacc gcggccggcc accacacacc gtcggcagtg
     2041 caggcgatgg ccgcgtacac gccgcgcatc tgctcgttgc agtccgcccg gtggaggttg
     2101 gccgtgggaa agcccaactg gcggccgcgc tgatctccga gctcgaccat cgctgtgacc
     2161 ggtgatggaa ggacaccact gacgaactgc ggcttgctcg gatccatgcc tttacaatac
     2221 aaccaaccag ttggttgtca actgttgagc taccctgcgc accgagcaga tcccagccac
     2281 catcattggc ggatcaacca aacgctcaac tactcttgac ccgtcgtgac tgtgaccgac
     2341 atgatcgcac tggaggtgct gtgccagttc tgacctcgcg gaccgacgtt tcgagcgagc
     2401 aattcacgac caatcgggca ggcatgctcg gactcatcga ggct
//
LOCUS       DAORNF010000537         2434 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_32604_length_2434_cov_2.159731, whole genome shotgun sequence.
ACCESSION   DAORNF010000537 DAORNF010000000
VERSION     DAORNF010000537.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32604_length_2434_cov_2.159731"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="PLV68_17415"
     CDS             <1..273
                     /locus_tag="PLV68_17415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03082.1"
                     /translation="GNGTQRVIANDKLIFLPEDPASLGFTAWGLTATALELVNSAQSE
                     MSFSEGAGIVGVVIKEGPPFRQFTFVDACGMPVLEDPNLLLVADVA"
     assembly_gap    350..382
                     /estimated_length=33
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <383..637
                     /locus_tag="PLV68_17420"
     CDS             <383..637
                     /locus_tag="PLV68_17420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03083.1"
                     /translation="SAPTTTVSRGRPAASPPAPPPPVSTPAAGEHPKPPAAGAGSGVT
                     EWRAYAESLGIDVVDNATRADIQELVDAYEQTQAAAGGQP"
     gene            653..1045
                     /locus_tag="PLV68_17425"
     CDS             653..1045
                     /locus_tag="PLV68_17425"
                     /inference="COORDINATES: protein motif:HMM:NF020912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gp19/Gp15/Gp42 family protein"
                     /protein_id="HRE03084.1"
                     /translation="MANFATVNGVEQAWRALTPTEQAQADHYLALASALIRHEFASID
                     ARVAADPAGLGVLVKGVTVEMVKRVLINPEGLRSVQESIDDYSRTMTRDSSMSSGGLF
                     LTDLERTLLTPTSRRGAFSIAPVDEGCR"
     gene            1042..1452
                     /locus_tag="PLV68_17430"
     CDS             1042..1452
                     /locus_tag="PLV68_17430"
                     /inference="COORDINATES: protein motif:HMM:NF039652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6093 family protein"
                     /protein_id="HRE03085.1"
                     /translation="MSLEGMIAAARSRVADTFLDTARFARPSDGPRAWDPATQRSTPP
                     PATTVHVGACRAKKPSSEQNLELFGDVEVTVPRLIITLPWEAPEIRVGDVTTFLQSAD
                     RELLRWSFRIVAVAPTSTLMYRRLGAEQLVRTGV"
     gene            1452..1826
                     /locus_tag="PLV68_17435"
     CDS             1452..1826
                     /locus_tag="PLV68_17435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03086.1"
                     /translation="MANNAAFAITADFAELDDLQQLLAAARQRPASEREGALDRATDR
                     AATEMRDITRAYPSPLDELSQHIETAGTPLYRRIFTTKRQGHFLHFGSPTTGAPRDWA
                     HGPTQRAVDDMFRELSKAAEPW"
     gene            1820..2257
                     /locus_tag="PLV68_17440"
     CDS             1820..2257
                     /locus_tag="PLV68_17440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03087.1"
                     /translation="MVRYATDPVVTAVIATLTTVGLKVGDGIAPAGVGWGAGASGAAG
                     VDVFQAYAVVHLFRVEHDGSLVDPFDDGAPVISVSAYADTASHARRVGDIAHSALVDG
                     VVTVTGRSVMLVAPLDDASTDRLDDVQPPIWQDVRRYRISTTA"
BASE COUNT          391 a          873 c          768 g          369 t
ORIGIN      
        1 ggcaacggca cccagcgcgt catcgcgaac gacaagctga tcttcctgcc ggaagacccc
       61 gcctcgctcg ggttcacggc gtggggactc accgccaccg cgctcgagtt ggtcaactcg
      121 gcacagtcgg agatgtcgtt ctctgagggg gccgggatcg tcggcgtggt catcaaggag
      181 ggaccgccgt tccgccagtt cacgttcgtg gacgcgtgcg gcatgcccgt cctggaggat
      241 ccgaacctgc tcctcgtggc cgacgtcgca tgagccggcg actcgtccgc acggtgttcg
      301 tggaggatgc caacggcgaa gcggtcgcgt tcgggccgca ttcgccgatn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nntctgctcc cacgacgacc gtttccaggg ggcggccggc
      421 tgcatcgcca cccgctccac ccccgccagt ctcgacaccc gctgcgggtg agcatcccaa
      481 gccgcctgcg gccggggccg ggtctggggt gaccgagtgg cgcgcgtacg ccgagtcgct
      541 cggcatcgac gtggtcgaca acgcgacccg cgccgacatc caagagctcg tcgacgcgta
      601 cgagcagacc caagccgccg ctggcggcca gccgtaggag agaaggcgcg tcatggccaa
      661 cttcgccacc gtcaacggcg tcgagcaagc gtggcgtgcc ctcaccccca ccgagcaagc
      721 ccaagctgac cactacctgg cgctcgcgtc agcgttaatc cggcacgagt tcgcgtcgat
      781 cgacgcccgc gtcgccgccg accctgcggg gctcggcgtg ctcgtcaagg gcgtcacggt
      841 cgagatggtc aagcgtgtcc tgatcaaccc cgaaggactg cgatcggtgc aggaatcgat
      901 cgatgactac tcgcgcacca tgacccggga ctcgtcgatg tcgtccggtg gcctgttcct
      961 gaccgacctc gagcggacgt tgttgacgcc gacgtcacgg cgcggcgcgt tctcgatcgc
     1021 ccccgtcgac gagggctgcc ggtgagtctt gaggggatga tcgcggcagc gcggtcccgt
     1081 gtggctgaca ccttcctcga caccgcacgg ttcgccaggc catccgacgg cccgagggcg
     1141 tgggacccgg cgacgcaacg ctcgacacca ccgccggcca ccaccgtcca cgtcggtgcg
     1201 tgccgagcga agaagccgtc gtccgagcag aacctcgaac tgttcggcga cgtcgaagtc
     1261 accgtcccgc gactcatcat cacgctcccg tgggaagccc ccgagatccg agtcggtgac
     1321 gtcaccacgt tcctgcagtc agccgaccgg gagttgctcc gctggtcgtt ccggatcgtc
     1381 gctgtcgctc cgaccagcac gctcatgtac cgcaggctcg gcgccgagca actcgttagg
     1441 accggtgtct gatggccaac aacgcagcgt tcgcgatcac cgctgacttc gccgaactcg
     1501 acgacctgca acagctactc gccgctgcac ggcaacgccc agcgagcgaa cgcgaaggcg
     1561 cgttggaccg ggcgacggac cgggcagcca ccgagatgcg ggacatcacc cgcgcctacc
     1621 cgtcaccact cgacgagctc tcccagcaca tcgagaccgc gggcactccg ctgtaccggc
     1681 ggatcttcac caccaaacgc caaggccact tcctgcactt cggcagcccc acgaccggcg
     1741 cgccacgcga ctgggcccac ggcccaaccc agcgcgctgt cgatgacatg ttccgcgagc
     1801 tctccaaggc ggctgagccg tggtgagata cgccaccgat ccagtcgtca ccgcggtgat
     1861 cgccacgctg accaccgtag gcctcaaggt gggtgacggc atcgcccccg ccggggtcgg
     1921 atggggtgct ggtgcgtccg gtgccgcggg ggtggacgtg ttccaggcct acgcggtggt
     1981 gcatctgttc cgcgtcgagc atgacggttc gctcgtcgat ccgttcgacg acggtgcccc
     2041 ggtgatctcc gtgtccgcct atgccgatac cgcgtcgcac gcacgccgag ttggcgacat
     2101 cgcgcacagc gcgctggtcg acggggtcgt gaccgtgacg ggccgttccg tgatgctcgt
     2161 cgccccactg gacgatgcgt cgaccgaccg cttggacgac gtgcaaccgc cgatctggca
     2221 agacgttcgt cgctaccgga tcagcaccac agcctgacgc ctgacggcga ccaggacccc
     2281 cctcacacca ccaaccgaac ggaggccctc gatggcgctc ttgtccaagc agtcgccggg
     2341 gctcccgtcg tctgcactca acatggccac cgcctctggc ggtggcgaca ccgtcgtcgc
     2401 cgaaccggcc gagcagacac cgctggttgc cgca
//
LOCUS       DAORNF010000538         2430 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_32686_length_2430_cov_1.343579, whole genome shotgun sequence.
ACCESSION   DAORNF010000538 DAORNF010000000
VERSION     DAORNF010000538.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32686_length_2430_cov_1.343579"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            359..1198
                     /locus_tag="PLV68_17445"
     CDS             359..1198
                     /locus_tag="PLV68_17445"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HRE03088.1"
                     /translation="MLAQINELSTWASTQAVVATKDIGTRIDMVRDRLVNEGAYSDGD
                     LAAGQAPSDAGHSAYRLTNFITKLPVRGNAEQYASAFGLMLRSMDNIRSRVVMGFEPN
                     DTDADNSESGVVEVTGRQVEAWVEVPVVGVGWVAVVPTPERTETTLKSKSPSPPEPEY
                     ETQAPPPPPLVDPDFDAPAASRTKAEDTREPDDPTLTPTKNVTAPAASSGGGAGRIVA
                     VAAGSPLLVLALVIGIIVFLKARRRRRRQTRGTPNERVANGWLELTDHALGHADATDV
                     HSA"
     gene            1173..1580
                     /locus_tag="PLV68_17450"
     CDS             1173..1580
                     /locus_tag="PLV68_17450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03089.1"
                     /translation="MPPTSTRREAAQFVGAGATLLAERADQAVFGPGEPTDAEVEQYW
                     TDLHAQLVSMRSQLGVVARAKAAVNITSLRQARATRRAVRMASTLPRAPRPPGALQPV
                     SSNLPRPSAPMSGAPSHLPPPSLPNAPHERPLR"
     gene            1577..>2055
                     /locus_tag="PLV68_17455"
     CDS             1577..>2055
                     /locus_tag="PLV68_17455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03090.1"
                     /translation="MSLVVDFCGERHTVVAGTPFIVGREADLSIDDNPFLHRRFLSLE
                     QINGIWMLRNVGKQLTATAADASGHLEAFLAPGAALPIVLPELVVSFTAGPTTYSFMI
                     QHPAAEFAPALGEPDTDDFGDTTIGATQLTPDQKRLVVALAEPRLAGDGRGTVNLPSS
                     "
     assembly_gap    2056..2197
                     /estimated_length=142
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          363 a          773 c          801 g          351 t
ORIGIN      
        1 cgggctactt cgaacggatg ggcgatcaca tcgacagcga ttacggcggt gagctcgcga
       61 cgatcaccgt caccgtcaag ggctacaacg acgtgtgggt gcccgatgtc ggcgaggtgt
      121 tgtcgttgcg cttcacgggg gggccgcgtg acgacctgct caacgaggag ttgcgctaca
      181 accgggccac cgacacggcg gcctcgcgga tcgcgttgac cgacggcgac cgctacgtga
      241 tgacggtgcg cctgccgacc gagctcgatc ggctggccca ggccgagatc gacccacagg
      301 tgtcggtcgg tccaggccgg gcccgccgag ctgcaggatg caggtgtcgg tcggcccggt
      361 gcttgctcag atcaacgagt tgtcgacgtg ggcgagcacg caggcagtgg tggccaccaa
      421 ggacatcggc acgcggatcg acatggtgcg tgatcgtctg gtcaacgaag gtgcctacag
      481 cgacggcgat ctggctgccg gtcaggcccc gtccgacgcg ggccacagtg cgtaccgcct
      541 caccaacttc atcaccaagc tgcccgtgcg cggcaacgcc gaacagtacg cgtcggcctt
      601 tgggttgatg ttgcgctcga tggacaacat ccgcagccgg gtcgtgatgg gcttcgaacc
      661 caacgacacc gatgccgaca attcggagag cggtgtggtc gaggtcaccg gcaggcaggt
      721 cgaagcctgg gtcgaggtgc ccgtcgtcgg ggtcggctgg gtggctgtcg taccgacgcc
      781 cgagcggacc gagaccaccc tcaagtccaa gagccccagt ccgcccgagc ccgagtacga
      841 gacgcaggcg ccgcccccgc cgccgttggt cgaccccgac ttcgacgcgc ccgcggcgtc
      901 tcggaccaag gccgaagaca cccgcgagcc cgacgatccg acgctcaccc cgaccaagaa
      961 cgtgaccgcc ccggccgcga gcagtggcgg gggcgcgggg cggatcgtcg ccgtcgcggc
     1021 tggttcgccg ttgctggtgc tggcgttggt gatcggcatc atcgtgttcc tgaaggcgcg
     1081 tcgtcgtcga cgtcgccaga cgcggggcac gcccaacgag cgggtggcca acggttggtt
     1141 ggagctgacc gaccacgccc tcgggcacgc cgatgccacc gacgtccact cggcgtgaag
     1201 ccgcccagtt cgtcggcgcc ggcgcgacgc tgttggcgga gcgggccgac caggcggtgt
     1261 tcggccccgg tgagccgacc gatgccgaag tcgagcagta ctggaccgac ctccatgcgc
     1321 agttggtgtc gatgcgcagc caactcggtg tcgtcgcccg tgccaaggcg gcggtcaaca
     1381 tcacgtcgtt gcgccaagcc cgcgccaccc gccgcgcggt gcggatggcc tcgacgttgc
     1441 cacgggcccc gcgcccaccc ggcgcactgc agcccgtgtc gtcgaacctg cctcgcccat
     1501 cggcgccgat gtccggcgca ccatcgcacc tgccgccgcc ttcgctgccg aacgccccgc
     1561 acgaaaggcc gttgcgatga gcctcgtggt cgacttctgc ggcgagcgcc acaccgtggt
     1621 cgcgggtacg ccgttcatcg tcggtcgcga ggcggatctg tcgatcgacg acaatccgtt
     1681 cctccaccgc cggttcctgt cgttggaaca gatcaacggc atctggatgc tgcgcaacgt
     1741 cggtaagcaa ctcaccgcca cggcggccga tgcgtcgggg catctggagg cgttcctcgc
     1801 tccaggcgcc gcgctgccga tcgtgctgcc cgaactggtc gtgtcgttca ccgctgggcc
     1861 gacgacgtac tcgttcatga tccagcaccc cgccgccgag ttcgctccag cgcttggcga
     1921 accagacacc gacgacttcg gcgacacgac gatcggggcg acgcaactga cccccgacca
     1981 gaagcggttg gtggtcgcgt tggccgagcc gcgcctggcc ggtgacgggc gggggacggt
     2041 caacctgccg tcgtcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncgg ttggtggagt acgcggtcgc
     2221 cacgcggttg gtgacgcgtg gcgatctggc cctgctcgat cagggcgacg accgtgacgc
     2281 tggtattcgg cacgctggta attgggacgc tgaggagagg gacgcgggag acgatgatgg
     2341 cgagtgacga tcgggtcaaa gatcgtgtga tcgaggtgag cggccagggc accggccgca
     2401 tcgcggaaca actggtggcg atcgcgaccc
//
LOCUS       DAORNF010000539         2428 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_32712_length_2428_cov_1.909397, whole genome shotgun sequence.
ACCESSION   DAORNF010000539 DAORNF010000000
VERSION     DAORNF010000539.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32712_length_2428_cov_1.909397"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(55..324)
                     /locus_tag="PLV68_17460"
     CDS             complement(55..324)
                     /locus_tag="PLV68_17460"
                     /inference="COORDINATES: protein motif:HMM:NF017793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion target"
                     /protein_id="HRE03091.1"
                     /translation="MAGPFTGMDIPAVRQLAQQLTSKADEIQQIMTTLTNLLQNTQWV
                     GADRERYLADWQGSHVTALNNVINGLRDASQKATQNASEQEQASS"
     gene            complement(483..>2428)
                     /locus_tag="PLV68_17465"
     CDS             complement(483..>2428)
                     /locus_tag="PLV68_17465"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HRE03092.1"
                     /translation="LATQRPAGVIKGNLRANTNLRVALRMADESDSDDVIGTSQAATF
                     DPAIPGRGVAKSGPGRLTAFQSAYVGGHTSGEAPPAIIDVHDLYFGVGSLWELPAVAV
                     EPDEAPSGPNDISRIVATVAAAAETAGLPAPRKPWLPELAHTYRLEDLPTARTDTELV
                     FGVIDDPDAQAQPVTAFVPDTDVNMAVFGTGGSGKSTFLRTLAVAAGFAPARGGPCTV
                     YAIDFGSRGLQMLEEMPHVGAVINGDDNERIVRLLRQLKALVDERATRYAKLNASTLA
                     EYRDRSGDGAEARVLLLIDGFGAFRSTYEVGPMAWVFEMLLSIAAAGRSAGIHVVVTA
                     DRPSSVPASLASSVQKRLVLRLANDTDYSLVGISPDAFGSASPPGRGFIDEFEVQVGV
                     LGGDSNVARQAAAVDRLTAAMLRAGVRPVERIASLPERVAASSLGPTAEGWPPLGVWD
                     ETLGPIGFEPVGTFLVAGPPQSGKTSTVGAMVAALRRSDPATKLALFGSRRSPLGAAI
                     EWDYRANTPDEMDTLAEELTPLVAAEDPSVRGLVVVIEQVSELLNSSADLALQGLLKA
                     CRSADHFVIAESESSTLGSSWPLLQAVKSSRTGIVLQPEQLDGDVIFKTPFPRVQRSE
                     FPPGRGFFVRAGKIARVHVALPE"
BASE COUNT          388 a          825 c          832 g          383 t
ORIGIN      
        1 cgaaacggcg ggaggctcca ccggaaccgt cccgccgctt cggcccccgc tcggtcagga
       61 cgaggcctgc tcttgctcgg aggcgttctg ggtggccttc tgcgacgcgt cacgcaggcc
      121 gttgatgacg ttgttcaacg ccgtcacgtg gctgccctgc cagtcggcga ggtaacgctc
      181 gcggtcggcg ccgacccact gcgtgttctg gagcaggttg gtgagggtgg tcatgatctg
      241 ctgaatctcg tcagccttcg acgtgagctg ctgcgccaac tggcgaaccg cagggatatc
      301 catacctgta aatggaccag ccatgagagt gtctccttgt ttgtgttgtt gctgggatcg
      361 tggcctcgtt gggaggaact acctcactca gaggaactga cggtcatggt aaagaacttg
      421 ccgcggcttg ggaatgggga gcactccccc accccggcgc cgagctcggt gtgcatctgc
      481 gcctactccg ggagggcgac atggacgcgg gcgatcttgc cggcacggac gaaaaagccc
      541 cgccctggcg ggaactcgct gcgctgcacg cgcgggaacg gcgtcttgaa gatgacgtcg
      601 ccgtcgagtt gctcgggctg cagcacgatg cccgtgcggc tcgacttgac cgcttgcaac
      661 aacggccatg aactgcccag cgtgctcgat tccgactcgg cgatcacgaa gtggtccgcg
      721 ctgcgacagg ccttgagcaa gccctgcaac gccaagtcgg cgctggagtt gagcagttcc
      781 gatacctgct cgatcaccac gaccaacccg cgcaccgacg ggtcttcggc agcgaccagc
      841 ggcgtcagtt cttcggccag tgtgtccatc tcgtcgggag tgttcgcgcg gtagtcccac
      901 tcgatcgccg caccgagcgg tgatcggcgg ctgccgaaca gggccagctt ggtggccgga
      961 tcggaacggc gcagtgcggc gaccatcgcc ccgacggtcg acgtcttgcc gctctgcggt
     1021 ggccccgcga ccaggaatgt gccgacgggt tcgaacccga tcggaccgag cgtctcgtcc
     1081 cacacaccca atggaggcca gccttcggcg gtcggcccca ggctggacgc ggcgacccgc
     1141 tcgggcaggc tggcgatccg ctcgaccggg cgcaccccgg cccggagcat cgccgcggtc
     1201 agacgatcga cggcggccgc ttggcgggcg acgttggagt cgccacccag cacgccgacc
     1261 tgcacttcga actcgtcgat gaaaccacgc cccggtggcg acgccgagcc gaacgcatcg
     1321 ggggagatgc cgacgagcga gtagtccgtg tcgttggcca aacgcagcac caagcgcttc
     1381 tgcaccgacg aggcgaggct ggccggcacg ctgctgggcc ggtcggcggt gacgaccacg
     1441 tggatgcccg cagagcgccc agcggccgcg atcgacaaca acatctcgaa cacccacgcc
     1501 atcggtccga cctcgtacgt cgagcggaat gcgccgaagc cgtcgatcaa cagcaacacc
     1561 cgggcctcgg caccgtcgcc gctgcggtcg cggtactcgg ccaaggtcga ggcgttgagc
     1621 ttggcgtagc gggtcgcccg ctcgtcgacc agcgccttga gttggcgcaa caaccgcacg
     1681 atccgctcgt tgtcgtcgcc gttgatcacg gcgccgacgt gaggcatctc ctccagcatc
     1741 tgcaagccgc gcgagccgaa gtcgatcgca tacacggtgc atggtccgcc gcgcgcgggt
     1801 gcgaaccccg ccgcgaccgc gagcgtgcgc aggaaagtgc tcttgcccga cccgccggta
     1861 ccgaacacgg ccatgttgac gtcggtgtcg ggcacgaacg ccgtgaccgg ttgagcctgc
     1921 gcgtcggggt cgtcgatcac gccgaacacc aactcggtgt cggtgcgcgc cgtgggcaga
     1981 tcctccagcc ggtacgtgtg ggccagttcg gggagccacg gcttgcgcgg cgcgggcagc
     2041 ccggccgtct ccgctgcggc ggcgacggtg gcgacgatgc ggctgatgtc gttggggccg
     2101 ctcggcgcct cgtcgggttc gaccgcgacc gccggaagtt cccagagcga accgacgccg
     2161 aagtacagat cgtgcacgtc gatgatcgcc ggtggcgcct cgcccgatgt atgcccaccc
     2221 acgtacgccg actggaacgc ggtcaggcga ccgggccccg acttggccac cccgcgaccg
     2281 gggatcgccg ggtcgaacgt ggcggcctgc gaggtgccga tcacgtcgtc gctgtccgac
     2341 tcgtcggcca tgcgcaacgc gacacgcagg ttggtgttgg cgcgcaggtt gcccttgatg
     2401 acaccggccg gacgctgcgt ggcaagga
//
LOCUS       DAORNF010000540         2427 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_32733_length_2427_cov_1.547639, whole genome shotgun sequence.
ACCESSION   DAORNF010000540 DAORNF010000000
VERSION     DAORNF010000540.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2427
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32733_length_2427_cov_1.547639"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>251
                     /locus_tag="PLV68_17470"
     CDS             <1..>251
                     /locus_tag="PLV68_17470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="coenzyme F420 hydrogenase/dehydrogenase beta
                     subunit N-terminal domain-containing protein"
                     /protein_id="HRE03093.1"
                     /translation="TTCTRACPRFRAWEPAADMHLFGKVREPDQMSGIWRQLLLTRAA
                     NDEEHQKGQDGGFVSAMLNWLLDNDYIEAALVSGVEEDD"
     assembly_gap    252..410
                     /estimated_length=159
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <411..995
                     /locus_tag="PLV68_17475"
     CDS             <411..995
                     /locus_tag="PLV68_17475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HRE03094.1"
                     /translation="WDRKAGKVSKPFLFNIGLLCSKTFDDAIFPELFEAKYGLKKQDM
                     VKMNIKGVFQIWMKDGSYHEIDLKECHAWTREGCKNCPDFSAEHADISTGGIGEDNDW
                     TLTIVRTELGEEVIRRMINDGTIIARPAQEDEKAMKLLRTLSIVSRRRWPEFADKAPT
                     VGVPPPKKKAAAPAAAADAPAPAAPAAEPATPAS"
     gene            complement(1003..1698)
                     /locus_tag="PLV68_17480"
     CDS             complement(1003..1698)
                     /locus_tag="PLV68_17480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HRE03095.1"
                     /translation="MNEAARRRQLHRQLAELGDEIHRCRACPRLVAWREQVACDKRAS
                     YRDEIYWGRPISGFGDPDARIVVLGLAPAAHGANRTGRIFTGDRSGDWLFRAMHRAGL
                     ANQAMSVGVDDGLELTDAWVTATVRCAPPDNKPEPSERDNCRPFLQRELALLPNAGVV
                     VCLGNFAYEAACREFGVRPRPKFGHLAEVVTPDGLHVLCSFHPSQQNTFTGRLTEPML
                     DAVFTRARALAGI"
     gene            1949..2425
                     /locus_tag="PLV68_17485"
     CDS             1949..2425
                     /locus_tag="PLV68_17485"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRE03096.1"
                     /translation="MDALYASPLERTRETAAPIAKALGLKTQIDRGLLECDFGDWTGA
                     ELKALMKLPEWTTVQRSPSTFRFPGGESFTEMQTRIVGALDRIRRAHPGGTVVCVSHA
                     DPIKADIAHAMGTHIDLFQRIVISTCSISAVAFHVGGPVGLTVNSTGTSLADLRPS"
BASE COUNT          394 a          826 c          736 g          312 t
ORIGIN      
        1 caccacctgc acccgcgcct gcccgcgctt tcgcgcctgg gagccggccg ccgacatgca
       61 cctgttcggc aaggtgcgcg agcccgacca gatgtcgggc atctggcgcc agctgctgct
      121 cacgcgcgcc gcgaacgacg aggagcacca gaagggccag gacggcggct tcgtgtcggc
      181 gatgctcaac tggctgctcg acaacgacta catcgaagcc gcgctcgtca gcggggtcga
      241 ggaggacgac gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tgggaccgca
      421 aggcgggcaa ggtgagcaag ccgttcctgt tcaacatcgg cctgctgtgc tcgaagacct
      481 tcgacgacgc gatcttcccc gagctcttcg aggccaagta cggcctgaag aagcaggaca
      541 tggtgaagat gaacatcaag ggcgtcttcc agatctggat gaaggacggc tcgtaccacg
      601 agatcgacct caaggagtgc cacgcctgga cccgcgaagg ctgcaagaac tgccccgact
      661 tctcggccga gcacgccgac atctccaccg gcggcatcgg cgaggacaac gactggaccc
      721 tcacgatcgt gcgcaccgag ttgggcgaag aggtcatcag gcggatgatc aacgacggca
      781 cgatcatcgc ccgcccggcc caagaggacg agaaggcgat gaagctgttg cgcacgctca
      841 gcatcgtcag tcgccgccgc tggcccgagt tcgccgacaa ggcgccgacc gtcggcgtcc
      901 ccccgccgaa gaagaaggcc gccgcacccg cggctgctgc cgacgccccg gctccggcag
      961 ctcctgctgc ggaaccggca acgccagcgt cgtagatcgg cgtcagatcc cggccagcgc
     1021 gcgggcgcgg gtgaacaccg cgtcgagcat cggctcggtc aagcggccgg tgaaggtgtt
     1081 ctgctggctg gggtggaacg agcacaacac atgcagcccg tcgggagtga ccacttcggc
     1141 caggtgcccg aacttgggtc gcggccgtac gccgaactcg cgacaggccg cctcgtaggc
     1201 gaagttgccc aagcacacca cgacccctgc gttcggcaac agcgcgagct ctcgctgcag
     1261 gaacggacgg cagttgtcgc gctctgacgg ctcgggcttg ttgtcgggcg gcgcgcaacg
     1321 caccgtggca gtgacccacg cgtcggtcag ttccaacccg tcatccacgc cgaccgacat
     1381 cgcctggttg gccagcccgg cacggtgcat cgcccggaac agccagtcgc cgctgcggtc
     1441 gccggtgaag attcgcccgg tccggttggc gccgtgcgcg gccggggcca ggccgagcac
     1501 cacgatccgc gcgtccgggt caccgaagcc ggagatcggc cgtccccagt agatctcgtc
     1561 gcgatacgac gctcgtttgt cacaggcgac ctgctcgcgc caggccacca gccgcggaca
     1621 ggcccggcag cgatggatct cgtcgcccaa ctcggccaac tgtcggtgga gttgccgtcg
     1681 ccgcgccgcc tcgttcacct gctccaccgt aggcccgcca gcaccagatc gccggggccg
     1741 cgtgaggccg gtcgtgttgg ccaggccgcg ttagcctggc cagggtcatg gcagcgaaga
     1801 agaccccacc cgcgcccaag cccaccgtcg tgctcctggt gcgccacggc cagacgccga
     1861 ccaccggcaa gctgttgccg ggtcgagcgc cgacgcgggg gtcgcccaag cccaagctgc
     1921 cgccaagcgc ctggccgaac tgccccatat cgatgcgctc tacgcatcgc cgctggagcg
     1981 cacccgcgag acggcagccc ctatcgcgaa agcgctgggg ctcaagaccc agatcgacag
     2041 gggcctgctc gaatgcgact tcggcgactg gaccggcgcc gagctcaagg cgctgatgaa
     2101 gctgcccgaa tggaccacgg tgcagcgctc gccgtccacg ttccgctttc ccggcggcga
     2161 gagcttcacc gagatgcaga cccgcatcgt cggcgccctc gaccgcatcc gccgcgccca
     2221 cccaggcggc acggtggtct gcgtcagcca cgccgacccc atcaaagctg acatcgcgca
     2281 cgcgatgggc acccacatcg acctgttcca gcgcatcgtc atctccacct gctcgatcag
     2341 cgccgtcgcc ttccacgtcg gcggaccggt ggggttgacg gtcaactcga ccggcacctc
     2401 gctggccgac ctgcgcccgt cctgacc
//
LOCUS       DAORNF010000541         2426 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_32753_length_2426_cov_1.958667, whole genome shotgun sequence.
ACCESSION   DAORNF010000541 DAORNF010000000
VERSION     DAORNF010000541.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32753_length_2426_cov_1.958667"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    209..221
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <222..>1101
                     /locus_tag="PLV68_17490"
     CDS             <222..>1101
                     /locus_tag="PLV68_17490"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRE03097.1"
                     /translation="GATTRTAIIDADAHIIEPADIWTSRLPSRLRDVAPRVERHPDTG
                     HEHWRIGSSWLWPVAQLAQAGWGEYPPSAPWDYADADPASFDATLRLARMDEYGIDVQ
                     VLYPNIIGFYAADIQELGADVAEMCVQAYNDFQIEWCSADPRRLVPVATIPFWDRDAS
                     VREMQRCAGLGFTSVLFANKFEQIGLPSFVDPYWDPIYAAAQDLDMPVNFHIGFAQSD
                     VRDNITADAIQSRRANVAQSKYNAVLNAGKTVMNQTFVIGNLLLSGVCDRFPRVKLVQ
                     VETGFGHIPFYLETLDW"
     assembly_gap    1102..1124
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1125..1407
                     /locus_tag="PLV68_17495"
     CDS             <1125..1407
                     /locus_tag="PLV68_17495"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03098.1"
                     /translation="SDLPLLPSEYFRRQCYSTFWFDRVSLPLLSAYPDNFMFSTDYPH
                     PISLSPGPCGGTDLLPSAWVADAFAGIDPVLTDKAVASNARAVYRLESV"
     gene            complement(1399..1710)
                     /locus_tag="PLV68_17500"
     CDS             complement(1399..1710)
                     /locus_tag="PLV68_17500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03099.1"
                     /translation="MSVDLKPGLRLLSAVDSTELIVVKAPSGPVELTVGGVPPAANAD
                     ARSTSGALSGHDGGVAIGKRYVDAGNTIELLCTKAGPGVPALGDELLTIKDAKPLPAS
                     D"
     gene            complement(1850..2389)
                     /locus_tag="PLV68_17505"
     CDS             complement(1850..2389)
                     /locus_tag="PLV68_17505"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRE03100.1"
                     /translation="MRQDADAVLDAAHDADAIAAAVRGRVVPAIVLTHGHNDHINAAV
                     ALQDAVDAPIYLHPDDTMLWHVVYPDREPDRALADRDVLRAGGAELGVIHTPGHAPGC
                     CCFHIVGAAADGSDLLFSGDTLFCGGPGATGRSYSDYDTIVASIRSRLFALPDSTIVH
                     TGHGDSTTIGAEAAALGRA"
BASE COUNT          407 a          813 c          764 g          406 t
ORIGIN      
        1 atcgatcgcc gggggaaccg acgagatcca gcgcaacatc atcggcgaga agatgctcgg
       61 tctcccccgc gaaccggcac gccccgaacc ggtacgcccc gaaccggcca cgtcctgaac
      121 tgtccactcc accctgccca cgtttctgag gagcgatcat gaccacaaca cttggcacac
      181 caccgcttgg cacttcgaca cttgccacnn nnnnnnnnnn ncggcgccac aacacgaacg
      241 gcgatcatcg acgccgacgc gcacatcatc gaacccgccg acatctggac ctcgcggcta
      301 cccagccgct tgcgtgacgt cgctccgcga gtcgagcgcc atcctgacac cgggcacgag
      361 cattggcgga tcggctcgtc atggctgtgg ccggtggcgc agctggccca agccggttgg
      421 ggtgagtacc caccgtcggc accgtgggac tacgccgacg cggacccagc cagcttcgac
      481 gcgacgctgc gcttggcccg catggacgag tacggcatcg acgtccaagt gctttacccg
      541 aacatcattg ggttctatgc cgccgacatc caggagcttg gcgccgacgt cgccgagatg
      601 tgcgtgcagg cctacaacga cttccagatc gaatggtgct cggccgaccc ccggcggctc
      661 gtgccggtcg caacgattcc gttctgggac cgcgacgcat cggtgcgcga gatgcaacgg
      721 tgcgccggtc tgggcttcac gtcggtgttg ttcgccaaca agttcgaaca gatcggtctg
      781 ccgtcgttcg tcgacccgta ctgggacccg atctatgcgg ccgcgcaaga cctcgacatg
      841 ccggtgaact tccacatcgg cttcgcccag agcgacgtgc gcgacaacat caccgccgac
      901 gccatccagt cccgccgggc caacgtcgcg cagtcgaagt acaacgctgt gctcaacgct
      961 ggcaagaccg tcatgaacca gaccttcgtc atcggcaacc tgctgctcag cggggtctgc
     1021 gaccgatttc cgcgggtcaa gctggtgcag gtcgagaccg gcttcgggca catcccgttc
     1081 tatctcgaga cgctcgactg gnnnnnnnnn nnnnnnnnnn nnnnttccga tctaccgctg
     1141 ttgcccagcg agtacttccg gcggcagtgc tattcgacgt tctggttcga ccgcgtgtcg
     1201 ttgccgttgc tgtccgcgta cccggacaac ttcatgttct cgacggacta tccgcacccg
     1261 atcagcctgt cgcccgggcc gtgcgggggc accgatctgc tgccctcggc atgggttgcc
     1321 gacgccttcg caggcatcga cccggtgctg accgacaagg ccgtcgccag caacgcccgt
     1381 gcggtctatc gactggagtc agtctgacgc aggcagcggc ttggcgtcct tgatcgtgag
     1441 gagctcgtca ccgagcgcgg gtacgccagg acccgccttg gtgcacagca actcgatggt
     1501 gttgccagca tcgacgtagc gcttgccgat cgcgacgccc ccgtcgtggc cgctcagggc
     1561 cccgctggtg gatcgcgcat cggcgttggc cgcgggtggc accccgccaa cggtgagctc
     1621 gacagggcca gacggggcct tgacgacgat caactcggtg ctgtcgacag cgctcaacag
     1681 gcgcagtccg ggtttgaggt ctaccgacat ggtgccgaat cctaccaacg atcaatttgg
     1741 tccaacccct ccggtttcaa ccacttggtc tgctccagtc ccccggcggc cggatcaggg
     1801 ctgatcggtg gcatcgaaaa cgatcacgcc gggaggcgac gtcgtgtgat caggcgcggc
     1861 cgagggcggc ggcttcggca ccgatcgtgg tgctgtcgcc gtggccggtg tggacgatgg
     1921 tggagtcggg cagggcgaac agtcggctgc gaatgctggc cacgatggtg tcgtagtcgc
     1981 tgtagctgcg ccccgtggca ccgggaccac cacagaacaa ggtgtcaccg ctgaacagca
     2041 gatcgctgcc atcggccgcg gcgccgacga tgtggaagca gcaacatccc ggcgcgtggc
     2101 ccggcgtgtg gatcacgccc aactcggcac cgccggcgcg gagcacgtcg cgatcggcca
     2161 aggcacgatc gggctcgcgg tcggggtaga ccacgtgcca cagcatcgtg tcgtctgggt
     2221 gcaagtagat cggcgcgtcg accgcgtcct gcaacgccac cgcagcgttg atgtggtcgt
     2281 tgtggccgtg ggtgagcacg atggccggca ccacgcgccc acggactgcc gcagcgatcg
     2341 cgtcggcgtc gtgcgccgcg tcaaggacgg catccgcatc ctgtcggatg gcgacctgaa
     2401 ggcaaaggtc tcgttcaatg tcgccg
//
LOCUS       DAORNF010000542         2424 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_32788_length_2424_cov_1.668637, whole genome shotgun sequence.
ACCESSION   DAORNF010000542 DAORNF010000000
VERSION     DAORNF010000542.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2424
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32788_length_2424_cov_1.668637"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            142..217
                     /locus_tag="PLV68_17510"
     tRNA            142..217
                     /locus_tag="PLV68_17510"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:175..177,aa:Arg,seq:cct)
     assembly_gap    298..449
                     /estimated_length=152
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(536..>865)
                     /locus_tag="PLV68_17515"
     CDS             complement(536..>865)
                     /locus_tag="PLV68_17515"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRE03101.1"
                     /translation="GEPITRDGLTMWPLTNRASGGLRTFKIRITSKRRRPPPRSELRV
                     HDGHDWMYVLDGRMRLILGEQDLIVEPGEAVEFSTLAPHWFGAIDGPVELIAVFGAHG
                     ERTHLHE"
     assembly_gap    866..875
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<876..1069)
                     /locus_tag="PLV68_17520"
     CDS             complement(<876..1069)
                     /locus_tag="PLV68_17520"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRE03102.1"
                     /translation="MSDRANIDISMLSRLESGKRRLALDHIPGLAAALSVSTDELLGG
                     APASDPRVRGVVLTLRGLSMS"
     gene            1210..>1729
                     /locus_tag="PLV68_17525"
     CDS             1210..>1729
                     /locus_tag="PLV68_17525"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRE03103.1"
                     /translation="MNRHLPHRAGSRVGDEPTTAPGRFAAFGNRNFRLYFIGQTISSI
                     GSWAQSLAVTWLVLDLTDRSDQLGIALALQFLPMLILGAPAGVLADRLNNRRLLLATS
                     AASGVLALTFGIVVTTGHATIWWIYALTFALGLVLAVERPTMQAILFQLVGRDLLSNA
                     VAANGTINSVSRL"
     assembly_gap    1730..1739
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1740..>2036
                     /locus_tag="PLV68_17530"
     CDS             <1740..>2036
                     /locus_tag="PLV68_17530"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRE03104.1"
                     /translation="AIAGGLIATVGVTIGFYINAASYLVVIAALLALRSSELVARPMI
                     GQTKGKLREGFAYVRNHPEVARPLIVMSIVGAIALNFPTTFPSMVRFGFDRGPGS"
     assembly_gap    2037..2120
                     /estimated_length=84
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2121..>2424
                     /locus_tag="PLV68_17535"
     CDS             <2121..>2424
                     /locus_tag="PLV68_17535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03105.1"
                     /translation="AGFGAAIVALGVMPTYATFVAMSIVLGFASASFQSVNTVVVQQA
                     TEPSMQGRVMALHQTALFGSTPIGALLMGWVIHVSSPRLPFLLGGLAGTGQLVLAAA"
BASE COUNT          361 a          736 c          656 g          415 t
ORIGIN      
        1 gtcgacgttc tggtccccgc tgttcggcac cgttaccgac cgcttcggca ccgcctggat
       61 gatctctgcc gaggacccca acgccccggg cgcttccccg cagtagtcac cccgtccagt
      121 gtcggtagat tgaccgcccc tgctcccgta gctcagggga tagagcgtcg gtttcctaaa
      181 ccgcaggtcg taggttcgat tcctaccggg ggcgccaaga aagaccagct cagtggtggt
      241 agctggtcga ctatcggagc gatctgtcca tcgagttgtc catctttggt ctcgccgnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnna cgtcggaacc gcagcacctt tcggtagccg
      481 gcgtacgacg ttgggcgttt gttcatcgtg cgcgaagcga gagcgtgctg gcagatcact
      541 cgtggaggtg ggtgcgttcg ccgtgcgctc cgaagactgc gatgagttca accggcccgt
      601 cgattgcgcc gaaccagtgg ggtgcgagtg tgctgaactc gacggcttcg cctggttcga
      661 cgatgagatc ttgttcgccg aggatgagac gcattcgtcc gtcaaggacg tacatccagt
      721 cgtgaccgtc gtggacacgg agctcactgc gcggcggtgg ccggcggcgc ttgctggtga
      781 tgcggatctt gaaggtgcgc aacccacctg aagccctgtt agtgagcggc cacattgtca
      841 ggccgtcgcg ggtgatcggc tcgccnnnnn nnnnngacat gctcaggccg cgcagcgtca
      901 gcaccacgcc gcggacgcgg ggatctgatg ccggggcacc gccgagcaac tcgtcggtgc
      961 tcactgacaa ggcggcagca agcccgggga tgtgatcgag agcgagacgt cgcttgcccg
     1021 actcgagcct gctgagcatc gagatgtcga tgttcgctcg atcactcacc tgttgcaagg
     1081 tgaggccctg ctcgtgacgg agctcgcgaa ggcgccgccg cactcgttca tcgatggact
     1141 caggggtcgg gtttgcctgt atggcaagaa cagttgctca caacgtggcg tgccgtcaag
     1201 gtcaacgtca tgaacagaca cctcccacac agggccgggt cccgagtcgg tgacgaaccg
     1261 acgaccgcgc cgggcagatt cgctgccttc ggcaaccgga acttccggct gtacttcatc
     1321 ggacagacca tctcgagcat cggctcatgg gcgcaatcgc tcgccgtcac ctggctggtc
     1381 ctcgacctca ccgaccgcag cgatcaactc ggcatcgccc tcgcactgca gttcctccca
     1441 atgctcatac tcggcgcacc ggccggcgtt ctcgccgacc ggctcaacaa ccgacgcctg
     1501 ctcctggcaa cctcagcagc atccggcgtg ctcgccctca cgttcgggat cgtcgtcact
     1561 accgggcacg ccacgatctg gtggatctac gcactcacct tcgcgctcgg cctcgtcctc
     1621 gccgtcgaac gaccaacgat gcaagccatc ctgttccaac tcgtcggccg agacctgctc
     1681 tccaacgcag tcgcagcaaa cggcacgatc aactccgtct cacgactcan nnnnnnnnnc
     1741 gcgatcgccg gcgggctgat cgccaccgtc ggtgtcacca tcggcttcta catcaacgcc
     1801 gcctcgtacc tcgtcgtgat cgccgccttg ctggcgttgc gttcgagcga actcgtcgcc
     1861 cggccgatga tcggtcagac caaaggaaag ctgcgtgaag gattcgccta cgtccgcaac
     1921 catcccgagg tcgcaaggcc gctgatcgtc atgtcgatcg tcggcgccat cgccctcaac
     1981 ttcccgacga ccttcccgtc gatggtccgc ttcggcttcg accgtgggcc cggctcnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn ggccggattc ggtgccgcca tcgtcgctct cggcgtgatg
     2161 ccgacctatg ccacgttcgt cgcgatgagc atcgtgctcg gcttcgcatc agcatcgttc
     2221 caatcggtga acaccgtcgt cgtccagcaa gcgaccgaac cgtcgatgca gggtcgggtg
     2281 atggcactgc accagacagc gctgttcggg agcacaccga tcggggccct gttgatgggc
     2341 tgggtcatcc acgtcagctc accacggctc ccgttcctgc tcggcggcct cgcaggcacc
     2401 ggccaactcg tgttggctgc ggcc
//
LOCUS       DAORNF010000543         2421 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_32853_length_2421_cov_1.345309, whole genome shotgun sequence.
ACCESSION   DAORNF010000543 DAORNF010000000
VERSION     DAORNF010000543.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32853_length_2421_cov_1.345309"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..535)
                     /locus_tag="PLV68_17540"
     CDS             complement(<1..535)
                     /locus_tag="PLV68_17540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03106.1"
                     /translation="MEPSRLTVIRRAFRSGSSEFWRGRLAGTAGFWRPVLIKRLTPEL
                     VADPRLAEQERSEALLTAHLVHPNILTVIEFGLFDGQPTTIHENVVAVDLLHTLIGLA
                     AGVERATAERRRLPGHLALHIVRQVLAALSAAHRHVEERLGFVGIAHGNLSPANILID
                     TRGHVLVRDFGIPLLPTP"
     assembly_gap    636..645
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<646..1525)
                     /locus_tag="PLV68_17545"
     CDS             complement(<646..1525)
                     /locus_tag="PLV68_17545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_function: GO:0120153 - calcium-dependent carbohydrate
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lectin domain-containing protein"
                     /protein_id="HRE03107.1"
                     /translation="MRRHLLPLSIALATFACGDSNTQDRDTTSADTTVTGDTNSDVTP
                     DTVENDTTADIPPDTTPGDDTTPDTTPGDDTAPDTTPADTADTTDPNACTQDFECVPR
                     FIVPPNACVEPRCIDHTCQLGPRLCDDSDPCTLDACDPRMGCLNVLMTVDVGPSQFLL
                     CQTPMTQAEAQTACTDRGLELASIDSEADASAVATLLADSGFDSAFIGAASTWLCPGK
                     RILPQVCRALPLDTCPIDESCATSIPFVCETHCNDGDPCTRDFVGPDGLCQSAEGGCD
                     DGDPCTTDVCDPRDGCL"
     assembly_gap    1639..1660
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1661..>2421)
                     /locus_tag="PLV68_17550"
     CDS             complement(<1661..>2421)
                     /locus_tag="PLV68_17550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03108.1"
                     /translation="LPASQCHNGSRVGVDQIYSGNRYSLQYPQAGTNLAMAENGDFFF
                     PIMQNGPAKGPKGIVRIKADGSTCEWVTRFAATSENIYAPKPEGQRGADYGLPNGTGP
                     RGDGPINYGQNPANIFYRRDASNIPWIYVMDGIASGAFGNRYYRVNVTTGDRFDLFSD
                     VVGDTYSEWDPHRQVLWTAGAFDRTRIVAVDILGLGGQPQVLGGIRCLSTNSDWYQCM
                     RGPGDVSNAARADVMFDPFDNNLIDDDVGHVRADD"
BASE COUNT          330 a          602 c         1047 g          410 t
ORIGIN      
        1 gtggggttgg cagcagcggg atgccgaagt cgcggacgag gacgtggccg cgtgtgtcga
       61 tgaggatgtt ggcgggcgag aggttgccgt gtgcgatgcc gacgaagccg agtcgctctt
      121 cgacgtggcg gtgggcggcg gacaaagccg cgaggacctg gcgcacgatg tggagggcga
      181 ggtggccggg gaggcgtcgg cgctcggccg tggcgcgctc gacgccggcg gccaggccga
      241 tcagggtgtg gagcaggtcg acggcgacga cgttttcgtg gatggtcgtg ggttggccgt
      301 cgaagaggcc gaactcgatg acggtcagga tgttggggtg gacgaggtgg gcggtgagca
      361 gggcctcgga gcgctcttgt tcggcgagtc gcgggtcggc gacgagctcg ggggtgaggc
      421 gcttgatgag gacggggcgc cagaagccgg cggtgccggc gaggcggccg cgccagaact
      481 cggaggagcc gcttcggaag gcgcgccgga tgaccgtgag gcgtgagggt tccacgccct
      541 gggtttagcg ccgtcgccgg gattcgtcca atggatgagt ggggcgggcg accaggtggg
      601 cctcaggggt cggcggggca gccggtgata ggcgtnnnnn nnnnnggagg cagccgtcgc
      661 gcgggtcgca gacgtcggtg gtgcaggggt cgccgtcgtc gcagccgcct tcggccgatt
      721 ggcagaggcc gtcggggccg acgaagtcgc gggtgcaggg gtcgccgtcg ttgcagtggg
      781 tttcgcagac gaacgggatg ctggtggcgc aggactcgtc gatggggcag gtgtcgagtg
      841 ggagggcgcg gcagacctgg gggaggatgc gcttgccggg gcagagccag gtgctggcgg
      901 cgccgatgaa ggcggagtcg aaaccggagt cggcgagaag ggttgcgacg gcggaggcgt
      961 cggcctcgct gtcgatggag gcgagctcga ggccgcgatc ggtgcaggcg gtctgggcct
     1021 cggcctgggt catcggggtc tggcagagga ggaactggga ggggccgacg tcgacggtca
     1081 tcagcacgtt gaggcagccc atgcgggggt cgcaggcgtc gagcgtgcag gggtcggagt
     1141 cgtcgcagag gcggggcccg agctggcagg tgtggtcgat gcagcgcggc tcgacacagg
     1201 cgttgggcgg gacgatgaag cgcgggacac actcgaagtc ctgggtgcag gcgttcgggt
     1261 cggtggtgtc ggcggtgtcc gcgggtgtgg tgtcgggggc ggtgtcgtcg ccgggcgtgg
     1321 tgtcgggggt ggtgtcgtcg ccgggcgtgg tgtcgggcgg gatgtccgcg gtggtgtcgt
     1381 tctcgacggt gtcgggtgtg acgtcggagt tcgtgtcgcc ggtgaccgtg gtgtcggccg
     1441 aggtggtgtc gcgatcctgg gtgttggagt cgccgcaggc gaaggtggcg agggcgatcg
     1501 agagcgggag gaggtgacgg cgcatgacgg ctccgggggt gaacgtggtt caccgtctag
     1561 ggcccggggt gtaggcgtgt caaacaaaag ggcggtcacc tggtggggtg accgcccttg
     1621 ttcgggtgcc gtcgtgaann nnnnnnnnnn nnnnnnnnnn atcgtcggca cgaacgtggc
     1681 caacatcgtc atcgatgagg ttgttgtcga aggggtcgaa catgacgtcg gcgcgggcgg
     1741 cgttgctgac gtcgccgggg ccacgcatgc actggtacca gtcggagttg gtcgaaaggc
     1801 agcggatgcc gccgagcacc tggggttgac cgccgaggcc gaggatgtcg acggccacga
     1861 tgcgcgtgcg gtcgaaagcg cctgcagtcc agaggacttg tcggtgcgga tcccattcgg
     1921 agtaggtgtc tcccacgacg tccgagaaca gatcgaagcg gtcgccggtc gtgacgttca
     1981 cgcggtagta gcggttgccg aaagcgcccg aggcgatgcc gtccatcacg tagatccacg
     2041 ggatgttgct ggcgtcacgg cggtagaaga tgttggcggg gttctggccg tagttgatgg
     2101 ggccatcacc gcgtgggccg gtgccgttgg ggagtccgta gtcggcgccg cgttggcctt
     2161 cgggtttcgg cgcgtagatg ttctcgctgg tcgcggcgaa gcgggtcacc cactcgcagg
     2221 tcgagccgtc ggccttgatg cggacgatgc ccttggggcc tttggcgggg ccgttctgca
     2281 tgatggggaa gaagaagtcg ccgttttcgg ccatggccag gttggtgccg gcttgtgggt
     2341 actgcaggct gtagcggttg ccgctataga tctggtcgac gccgacacgg ctgccgttgt
     2401 ggcactggct cgcgggaagg g
//
LOCUS       DAORNF010000544         2418 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_32919_length_2418_cov_1.503597, whole genome shotgun sequence.
ACCESSION   DAORNF010000544 DAORNF010000000
VERSION     DAORNF010000544.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32919_length_2418_cov_1.503597"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            7..135
                     /locus_tag="PLV68_17555"
     CDS             7..135
                     /locus_tag="PLV68_17555"
                     /inference="COORDINATES: protein motif:HMM:NF014636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HRE03109.1"
                     /translation="MNELGLNRESPLVIRNGTLVASDTELAADDVIEIRPVISGGI"
     gene            174..>908
                     /locus_tag="PLV68_17560"
     CDS             174..>908
                     /locus_tag="PLV68_17560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine biosynthesis TtcA family
                     protein"
                     /protein_id="HRE03110.1"
                     /translation="MPRHNANFCAPHFLDMCRRQVVKAIEDHDMLSRDDRALVAVSGG
                     KDSLAVWDLLIDLGYQADGLYVGLGIGDYSDVSGEHARAFAAERGLTLIEVSLRDDYG
                     YDIPTATRATRRVPCSACGMSKRHIFDKAALDGGYTVLVTGHNLDDEAAVLYGNTLRW
                     DMEYLARQLPVLPARNGFPKKVKPLMRLSEREMAAWCVIRGIDYLVEECPIAAGNRHL
                     GYKEALNAIETTSPGTKAAFYLSFVER"
     assembly_gap    909..926
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1135..1259
                     /estimated_length=125
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1260..1910
                     /locus_tag="PLV68_17565"
     CDS             <1260..1910
                     /locus_tag="PLV68_17565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
                     /protein_id="HRE03111.1"
                     /translation="VVVEMPRGAQVIYPKDLAPMCMLADIGPGVKVFESGVGSGALSM
                     TMLRYGAHITGYELREDFANRAIANVGSFLGAQALERYDVHIRDAYEGIDGTDFDRVV
                     LDLPEPWQVVPHAERALRLGGILVAYTPSITQAVRVREALAASRAWNGARTLEVLHRG
                     WYIEGAAVRPDHRMVAHTAFLTTARFLGTRPLGNRDEPAETSNAADAPNTETGEND"
     gene            complement(1971..2216)
                     /locus_tag="PLV68_17570"
     CDS             complement(1971..2216)
                     /locus_tag="PLV68_17570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HRE03112.1"
                     /translation="MKVWIDQDLCTGDGLCEEICPDVFTLLDDGLAYVKEGATIYAEA
                     KGNAQGAEGMANIPDNQLDAVIESAEECPGECIFIEP"
BASE COUNT          384 a          755 c          751 g          385 t
ORIGIN      
        1 gcgttgatga acgaactcgg cctcaaccgc gagtctcccc tcgtcatccg caacggcacc
       61 ttggtggcca gcgacaccga gctggccgcc gacgatgtga tcgagatccg tccggtcatc
      121 agcggcggca tctgatgaag tgccgcgtct gccgggggcc ggcgatcatc gatgtgccgc
      181 gccacaacgc caacttctgc gcgccgcact tcctcgacat gtgccgccgc caggtggtca
      241 aggcgatcga ggaccacgac atgctcagcc gcgacgatcg cgctttggtc gccgtttcag
      301 gcggcaagga cagcctggca gtctgggacc tgctgatcga tttgggctac caagccgatg
      361 ggctgtacgt cgggctcggc atcggtgact acagcgatgt gtcgggcgag catgctcgcg
      421 cgttcgccgc cgagcgcggc ttgacgttga tcgaggtgtc gctgcgcgac gactacggct
      481 acgacatccc caccgccacc cgggccacgc ggcgggtgcc gtgctcggca tgcggcatga
      541 gcaagcgcca catcttcgac aaggccgcgc tcgacggcgg atacacggtg ctggtcacgg
      601 gccacaacct ggacgacgag gcggccgtgc tgtacggcaa cacgctgcgg tgggacatgg
      661 agtacctggc ccgtcaactg ccggtgctgc ccgcccgcaa cggcttcccc aagaaggtca
      721 agccgctgat gcgcctgagc gagcgggaga tggcggcctg gtgtgtgatc cgcggcatcg
      781 actacctggt cgaggaatgc ccgatcgcgg ccggcaaccg ccacctcggc tacaaggaag
      841 cgctcaacgc gatcgagacg acgtcaccgg gcaccaaggc tgcgttctac ctcagcttcg
      901 tcgagcggnn nnnnnnnnnn nnnnnncgac accgaactgc acgcctgtgc acgctgcggg
      961 gcaccgacca ccggcgaggt ctgcgcgttc tgcaacctgg tcgacaagtc ggcggcgcac
     1021 gaaccggtgc ccgtcgagat gatcaccgga aagggacggc ggcgatgaac cacccatttg
     1081 cctacggcga gcgggcgttg ctgctcgaca ccaaacagcg gcggtacttg atcgnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng
     1261 tcgtggtcga gatgccgcgc ggggcccagg tgatctatcc caaggacttg gcgccgatgt
     1321 gcatgttggc cgacatcggc cccggcgtga aggtgttcga gtcgggcgtc ggcagcggcg
     1381 cgctgtcgat gacgatgttg cgctacggcg cccatatcac cggctacgaa ctacgcgagg
     1441 acttcgccaa ccgcgcgatc gccaacgtcg gttcgttcct cggtgcccag gcgctggaac
     1501 gctacgacgt ccacatccgc gacgcgtacg agggtatcga cggcaccgac ttcgaccggg
     1561 tggtgctcga cctgcccgag ccgtggcagg tggtgcccca cgccgagcgg gcgctgcgcc
     1621 tcggcggcat cctggtggct tacaccccct cgatcaccca ggccgtgcgg gtgcgtgagg
     1681 cgctcgccgc gagccgggcc tggaacggcg cgcgcacgct cgaggtgctg catcgcggct
     1741 ggtacatcga aggcgccgcc gtgcgccccg atcaccgcat ggtcgcccac acggcgttct
     1801 tgaccaccgc ccgcttcctc ggcacccgcc cgttgggcaa tcgggacgag ccggcggaga
     1861 cgtcgaacgc cgccgatgct cccaacacag agaccggcga aaacgactga agggacgccg
     1921 cggcgtccct tcaggtctcg atcgttgatg ctgaccgcgt cagcgtggtg ttacggctcg
     1981 atgaagatgc actcgccggg gcactcctcg gcgctctcga tgacggcatc gagttggttg
     2041 tctgggatgt tggccatgcc ctcggcgccc tgggcgttgc ccttggcttc ggcgtagatg
     2101 gtggcgcctt ccttcacgta cgccaaaccg tcgtcgagca gggtgaatac gtccgggcag
     2161 atctcttcgc agagtccgtc tcccgtgcag agatcctgat caatccagac cttcatggtg
     2221 ggtgtgtctc cttcggaggc tgagagaagg cggtacgaaa cgttgatcac tgtacacggg
     2281 ttcgctcgtt tccaaggatt tcccccagct cagcatcacg gtcgagagac acacgcgatc
     2341 cggccaggcc gtgtaggttc ggcatcgagg cgcaccgttc gctcaaccag tgaggagtca
     2401 tcgtgaccaa ctcttcca
//
LOCUS       DAORNF010000545         2412 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_33019_length_2412_cov_1.444209, whole genome shotgun sequence.
ACCESSION   DAORNF010000545 DAORNF010000000
VERSION     DAORNF010000545.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33019_length_2412_cov_1.444209"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="PLV68_17575"
     CDS             complement(<1..328)
                     /locus_tag="PLV68_17575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03113.1"
                     /translation="MSAEEMRVTTQAPFVPGTASSAPLQQRILRSDAVRFVAPLMIAI
                     IAISIYTNNKNGNFLGSDNIKNILLQVSVLGIIAIGQTFLIAAGQLDLSVATLAALAG
                     VIGATRI"
     gene            complement(325..>520)
                     /locus_tag="PLV68_17580"
     CDS             complement(325..>520)
                     /locus_tag="PLV68_17580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03114.1"
                     /translation="YAELRRLADRGAAVLICSTDFQEVGQVADRVIVLRSGRVVGEVA
                     GNEATEHHLLELESGLVMSQ"
     assembly_gap    521..530
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<531..>1020)
                     /locus_tag="PLV68_17585"
     CDS             complement(<531..>1020)
                     /locus_tag="PLV68_17585"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRE03115.1"
                     /translation="SLTVAAGEIVAVTGGVGAGVSDVARVAAGGMSYGAGSVSVADAA
                     GAMRRIRNRRHALSLGVAFLPADRKRQGLLLERTVADNIVLGQQADGRSVVLAPTSVR
                     RSAVKLVPESRVKAATVDVTVGTLSGGNQQKVMIGRWLGVDSKVLIFDEPTAGIDIAS
                     KFE"
     assembly_gap    1021..1047
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1048..>1457)
                     /locus_tag="PLV68_17590"
     CDS             complement(<1048..>1457)
                     /locus_tag="PLV68_17590"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="with RbsBCD acts to import ribose into the cell;
                     RbsA contains 2 ATP-binding domain; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HRE03116.1"
                     /translation="DLEIDLDINAPVRSLTVAQLQMMEIARAVSFDARLIVMDEPTAS
                     LSRHEVEPLFRVINRLREAGVAILFISHHLDEVFSIADDVTVMRDGEVVANGPAATFT
                     PARVVEAMFGRQVDINRVDRPAAQAVGAAMLALD"
     assembly_gap    1458..1507
                     /estimated_length=50
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1508..>1753)
                     /locus_tag="PLV68_17595"
     CDS             complement(<1508..>1753)
                     /locus_tag="PLV68_17595"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRE03117.1"
                     /translation="AVRAGTVHALCGENGAGKSTLIKIATGAQPADSGTISVDGEVVT
                     HPNRRQMQALGIRAIFQERQIAADLSVAENVLLDRLPT"
     assembly_gap    1754..1851
                     /estimated_length=98
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1939..>2412)
                     /locus_tag="PLV68_17600"
     CDS             complement(1939..>2412)
                     /locus_tag="PLV68_17600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HRE03118.1"
                     /translation="AAAAEAAGRNPDDVTICVAAPAYVTDGSAEHLAHAREQCRWFGG
                     MVGNHVADIVSRYGDEAPVPKALTDYIKGREGYDYNEHGQAGNTHTSFVPDEIVDRFC
                     IVGPVDEQVRRLEELKALGVDQFSIYLQHDAKDETLRAYGERVMPAIADTVLAKS"
BASE COUNT          378 a          737 c          731 g          381 t
ORIGIN      
        1 tgatgcgggt tgcgccgatc acgccagcga gcgcagccaa cgtcgcgacg gagaggtcga
       61 gttggccggc cgcgatcaag aacgtctggc cgatcgcgat gatgccgagc accgacacct
      121 gcaacaggat gttcttgatg ttgtcgctac cgaggaagtt gccgttcttg ttgttggtgt
      181 agatcgagat cgcgatgatc gcgatcatca acggagcgac gaatcggacc gcgtctgagc
      241 gcaggattcg ttgctggagc ggggcgctcg acgcggtccc tggcacgaat ggggcctgtg
      301 ttgtcacccg catctcctcg gcactcattg cgacatcacc aatcctgatt cgagctccaa
      361 cagatggtgc tcggtggcct cgttaccggc cacctcgccg accacgcggc cggaccgcag
      421 cacgatcacc cggtctgcca cttgtccgac ctcttgaaag tcggtggaac agatcaacac
      481 agcagctccc cgatcggcca ggcgccgcaa ctcggcatag nnnnnnnnnn ctcgaacttg
      541 gacgcgatgt cgatgccggc cgtgggctcg tcgaagatca gcaccttgct gtcgaccccc
      601 aaccagcgac cgatcatcac cttctgttgg ttgccgcccg agagcgtgcc gacggtgacg
      661 tcgaccgtgg cggccttgac ccgcgattcg ggaacgagct tgacggcaga tcgccggacg
      721 ctggtcggag ccagcaccac cgaccggcca tcggcctgct ggccgagcac gatgttgtcg
      781 gcaacggttc gctcgaggag caagccctgg cgcttgcgat cggccggtag gaacgcgaca
      841 ccgagcgaca gcgcgtgacg gcgattcctg atccggcgca tcgctcctgc ggcgtcggcc
      901 accgacaccg aacctgcgcc gtacgacatc ccgccagcgg caacccgggc gacatcggac
      961 acccctgcgc cgacaccgcc ggtgaccgcc acgatctctc ccgccgcgac ggtcaaggag
     1021 nnnnnnnnnn nnnnnnnnnn nnnnnnnatc gagggccaac atggcggcgc caacggcctg
     1081 tgcggcagga cgatcgacgc ggttgatgtc gacctggcgg ccgaacatcg cttcgacgac
     1141 acgggccggc gtgaacgtgg cggccggccc gttcgcgacc acctcgccat cgcgcatgac
     1201 cgtgacgtcg tcggcgatgc taaagacctc gtcgaggtga tggctgatga acaggatggc
     1261 gacacctgcc tcgcgcaacc ggttgatcac gcggaacagc ggttcgacct cgtggcggct
     1321 gagcgacgcc gtgggctcgt ccatgacgat cagccgcgcg tcgaagctga cggcccgagc
     1381 gatctccatc atctgcaact gagcgacggt cagcgaccgc accggggcgt tgatgtcgag
     1441 atcgatctcc aagtccgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnngtc ggcaggcggt cgaggagcac gttctcggca acggacagat cggctgcgat
     1561 ctggcgctct tggaagatcg cccggatccc gagcgcttgc atctggcgac ggttggggtg
     1621 ggtgacgacc tcaccgtcga cggagatcgt gccgctgtcg gccggctggg caccggtcgc
     1681 gatcttgatc agcgtcgact ttcccgcacc gttctcgcca cacagcgcgt gcacggttcc
     1741 tgcccgcacg gcgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntcctcgcac
     1861 gcggcgctgc acgatcgttc ggtatgtggt gttcatcgtc gtgtcacacc tccagcaacc
     1921 cggctgtccc acgcgaacct acgacttggc caagacggtg tcggcgatgg cgggcatgac
     1981 ccgctcaccg taggcccgca gtgtttcgtc cttggcatcg tgttgtaaat agatcgagaa
     2041 ctgatcgacg cccagcgcct tgagctcttc gaggcggcgg acctgttcgt cgaccggccc
     2101 gacgatgcag aaccgatcga cgatctcgtc cggaacgaac gaggtgtggg tgttacccgc
     2161 ctggccgtgc tcgttgtagt cgtacccctc gcggcccttg atgtagtcgg tcaacgcctt
     2221 gggtaccggg gcctcgtcgc catagcggga cacgatgtcg gcgacgtggt tgccgaccat
     2281 gccgccgaac cagcggcact gctcgcgggc gtgggcgaga tgctcggccg aaccgtcggt
     2341 gacgtaggcc ggggcggcga cacagatcgt gacgtcgtcg gggttgcgac ccgccgcctc
     2401 ggccgcggcg gc
//
LOCUS       DAORNF010000546         2412 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_33022_length_2412_cov_1.348748, whole genome shotgun sequence.
ACCESSION   DAORNF010000546 DAORNF010000000
VERSION     DAORNF010000546.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33022_length_2412_cov_1.348748"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>341)
                     /locus_tag="PLV68_17605"
     CDS             complement(<1..>341)
                     /locus_tag="PLV68_17605"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441861.1"
                     /note="catalyzes the interconversion of D-ribulose
                     5-phosphate to xylulose 5-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HRE03119.1"
                     /translation="ADYVKAGCQRLIVHAEACLHLHRTLGHIRSLGANAAVALNPATP
                     AATIAHVLDLVDLVLVMTVNPGFGGQAYIATMEPKIREIRTMIEDAGLTDQIDLEVDG
                     GISASTVGGAA"
     assembly_gap    342..389
                     /estimated_length=48
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<390..568)
                     /locus_tag="PLV68_17610"
     CDS             complement(<390..568)
                     /locus_tag="PLV68_17610"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF013030.2"
                     /note="catalyzes the interconversion of D-ribulose
                     5-phosphate to xylulose 5-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HRE03120.1"
                     /translation="MSRTIQIAPSVLPADFSRLGNEVAALEAAGVDLIQWDVMDGQFV
                     PNLTFGPDIIAAARP"
     assembly_gap    707..775
                     /estimated_length=69
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <776..1981
                     /locus_tag="PLV68_17615"
     CDS             <776..1981
                     /locus_tag="PLV68_17615"
                     /inference="COORDINATES: protein motif:HMM:NF014047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HRE03121.1"
                     /translation="GVERVRVTVERMVVGGDGLGREADGRVVFVRGAIVGETVDVEVV
                     ERRRDFARAEVSRVVEPSPWRVEPPCAAWHNGCGGCDWQHVDSAAQLGLKVDIVREAL
                     ARTAHLPDAEVVAAGSVASWGYRTTVRLAGRADGRVGFRGRRSRGVIAIDECMVAADP
                     INAALAAGRIDGRRALRRDVLARVGTDDDRVALHAIDAQRDQPSLRATSHVTRGRGRG
                     GVVVNEVAGARLRVSADSFFQSSAAAAELLVAAVERCCADLDLATATVVDAYGGVGLF
                     GATVGRDAPRLIVVESSPSSSADARLNLGDRAGVQIVQSTVERWTPQPADLVIADPAR
                     SGLGREAAAVLAATQTPRLVLVSCDVGSLTRDVRLLTDLGYEHRGTQVLDLFPNTSHV
                     EAVTRFDRA"
     gene            2168..>2412
                     /locus_tag="PLV68_17620"
     CDS             2168..>2412
                     /locus_tag="PLV68_17620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HRE03122.1"
                     /translation="MLHTVLSPEVADAIASRSPIVAMESTIFSHLGLPTPFNEQALDR
                     CIAAVRAVGAVPAVTAVLDGVARVGLDASEHERILGAA"
BASE COUNT          293 a          727 c          871 g          404 t
ORIGIN      
        1 ggccgcgccg ccgacggtgc tggcgctgat accgccgtcg acttcgagat cgatctggtc
       61 ggtgaggcca gcgtcttcga tcatggtccg gatctcgcgg atcttcggct ccatcgtggc
      121 gatgtaggcc tgaccgccga aaccggggtt gaccgtcatc acgagcacca agtcgaccag
      181 gtcgagcaca tgcgcaatgg tcgcagcggg tgtcgctgga ttgagcgcga ccgccgcgtt
      241 ggcgccgagg ctgcggatgt ggccgagcgt gcggtggagg tgcaggcacg cttcggcgtg
      301 cacgatcagg cgctggcatc cggccttcac gtagtccgcc gnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt gcgggcgagc ggcggcgatg atgtcgggac
      421 cgaaggtcag gttggggacg aactggccgt ccatcacatc ccactggatc agatcgacac
      481 ccgctgcctc cagcgccgcg acctcgttgc caaggcggga gaagtcggcg ggcagcaccg
      541 acggtgcgat ctgaatcgtg cggctcatcg ccgccaactg tagccctgcc ctcgtcgcgc
      601 caccccgccc actcccaccc ggtgagtcgg gcgtccgcgg agcgtggatc ggccggtatc
      661 cggtgcattg gtactccgcg gaggccggat tgcccggggt ggtggannnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnggggt
      781 ggagcgggtg cgggtgacgg tcgagcggat ggtggtcggc ggcgacggac tcgggcggga
      841 ggcggatggg cgggtggtgt tcgtacgcgg ggcgatcgtg ggggagacgg tcgacgtcga
      901 ggtcgtggaa cggcggcgcg acttcgcccg cgccgaggtc tcacgggtgg tggagccgtc
      961 gccgtggcgc gtcgagccgc catgcgcggc ctggcacaac gggtgcgggg gttgcgactg
     1021 gcagcacgtc gattccgccg cgcagttggg gctgaaggtc gacatcgtgc gcgaggcgct
     1081 ggctcgtacg gctcacctac ccgacgcgga ggtggtcgcc gctgggtcgg tcgcgtcatg
     1141 gggataccgg acgaccgtgc gcctcgccgg tcgtgccgat gggcgggttg gcttccgcgg
     1201 gcggcgttca cgtggcgtga tcgcgatcga cgagtgcatg gtcgcggccg acccgatcaa
     1261 tgcggcgctg gctgctggtc gcatcgacgg ccgtcgcgcc ttgcgccgcg acgtgctcgc
     1321 ccgagtcggc acggacgacg accgcgtcgc gctccacgcc atcgacgccc aacgtgacca
     1381 gcccagcctc cgcgcaacga gccatgtcac tcgtggtcgt ggccgcggtg gggtggtggt
     1441 gaatgaggtg gcgggggcgc ggttgcgggt atcggcggat tcgttctttc agtcgtcggc
     1501 ggctgccgcg gaactgttgg tcgccgcggt ggagcgatgt tgtgccgatc tcgacctggc
     1561 gacggcgacg gtggtcgacg cctatggcgg ggtcgggttg ttcggcgcga cggtcgggcg
     1621 cgacgcgccg cggctgatcg tggtggagtc gtcgccgtcg tcatccgctg acgcacgcct
     1681 caatctgggt gaccgcgctg gggtgcagat cgtgcagtct acggtcgagc gttggacgcc
     1741 acagccggcc gatctggtga tcgccgatcc ggctcggtca gggctgggtc gcgaggcggc
     1801 cgcggtgttg gccgcgaccc agacgcctcg cctggtgttg gtgagttgtg acgtcgggtc
     1861 gcttactcgc gacgtgcggc tgctgactga cttgggctac gagcatcggg gcacccaggt
     1921 gctcgatctg ttccccaata cctctcacgt cgaagccgtc actcgattcg accgcgcctg
     1981 acgcggcacg gtgtgggggt ttgggacggc cgcctcgacc ccacgtcgag gagcggtcct
     2041 gcatcgggtc ttcgcgggtt cacatgaagg cgatccctgc tggccggacc acgctcgatc
     2101 ccatgtctcc ctgtcgtcga tctgctggca aagattccat cgcgtctccc gctggcagac
     2161 tgtggccatg ttgcacaccg tgttgtcgcc cgaagttgcc gatgcgatcg cgtcgcgcag
     2221 cccgatcgtg gcgatggagt cgaccatctt ctcccacctc ggcctgccca cgccgttcaa
     2281 cgaacaggcg ctcgaccgct gcatcgcggc ggtgcgcgcg gtgggcgctg tcccggcggt
     2341 cactgcggtg ctcgacgggg tggctcgtgt cggcctcgac gccagcgagc acgagcgcat
     2401 cctcggtgcg gc
//
LOCUS       DAORNF010000547         2406 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_33125_length_2406_cov_1.350915, whole genome shotgun sequence.
ACCESSION   DAORNF010000547 DAORNF010000000
VERSION     DAORNF010000547.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33125_length_2406_cov_1.350915"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>249
                     /locus_tag="PLV68_17625"
     CDS             <1..>249
                     /locus_tag="PLV68_17625"
                     /inference="COORDINATES: protein motif:HMM:NF014010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PhoU domain-containing protein"
                     /protein_id="HRE03123.1"
                     /translation="SIPRATDILLSGDLEGADFVIRGDDEIDARSLDLEERCVEILTL
                     QSPVAVDLRQVLAAMRMIAEIERSADLVANICKAARRI"
     assembly_gap    250..388
                     /estimated_length=139
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <389..724
                     /locus_tag="PLV68_17630"
     CDS             <389..724
                     /locus_tag="PLV68_17630"
                     /inference="COORDINATES: protein motif:HMM:NF014010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoU domain-containing protein"
                     /protein_id="HRE03124.1"
                     /translation="MDNYLDALQKQFVQAIFESHARGNIDLQVAVQLAVVARFFERIG
                     DHAVNIGERVRYVVTGWLPEHRGAARYRSRDAASSGDASSGEASSSDASSSDAADATD
                     DRAGMPVDG"
     gene            732..>1351
                     /locus_tag="PLV68_17635"
     CDS             732..>1351
                     /locus_tag="PLV68_17635"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HRE03125.1"
                     /translation="MTTWLAAVVGIGIGVVAGAGIAVLVMRGRVAAAPDAGRDGSELA
                     LHQAQRVADRLQAALDALPVGVVVSDAAGRVVVRNRAADNSGGDTHNEVLVQAAVQHH
                     LRGALRGGPRSQTVELFGPPRRIVVVHARPLAGEGAVATIEDITERSRLDSVRTDFVA
                     NISHELKTPIGALAVLAETMIDEHDPDLIRRLAGKTVNEAHRAAGI"
     assembly_gap    1352..1478
                     /estimated_length=127
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1479..1869
                     /locus_tag="PLV68_17640"
     CDS             <1479..1869
                     /locus_tag="PLV68_17640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016297362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRE03126.1"
                     /translation="NVAPIAADLSLNADQGQLASALGNLVDNAVKYSEAGDTVTIAAV
                     RESNGVSFTVADSGIGIPARDLDRIFERFYRVDRARSRGTGGTGLGLSIVRHVANNHG
                     GAVTVASRGSLSDPAGRAEAMALIGAA"
     gene            1866..>2018
                     /locus_tag="PLV68_17645"
     CDS             1866..>2018
                     /locus_tag="PLV68_17645"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HRE03127.1"
                     /translation="MTHILGILNVTPDSFSDGGQFDEVDAAIARGRQLRDAGADLVDV
                     GGESTRP"
     assembly_gap    2019..2190
                     /estimated_length=172
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2191..>2406
                     /locus_tag="PLV68_17650"
     CDS             <2191..>2406
                     /locus_tag="PLV68_17650"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HRE03128.1"
                     /translation="GLADPDMYRVIAETKLTYIAMHSRGPSAETAAYEDVVAEVRADL
                     KARLAEMVVVGVDLNRVILDPGLGFAKD"
BASE COUNT          303 a          661 c          699 g          305 t
ORIGIN      
        1 aatcgatccc gcgcgcgacc gacatcttgc tgtcgggcga tctcgaaggc gccgacttcg
       61 tcatccgtgg cgacgacgag atcgatgccc gctcgctcga tctcgaagag cgttgcgtcg
      121 agatcctgac gttgcagtcg ccggtcgccg tcgacctgcg ccaggtgctg gcggcgatgc
      181 ggatgatcgc cgagatcgag cgctcggccg acctggtcgc caacatctgc aaggcggctc
      241 ggcgcatctn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnat ggacaactac ctcgacgcgc tgcagaagca
      421 gttcgtgcag gcgatcttcg agagccacgc ccgtggcaac atcgacctgc aggtcgccgt
      481 tcaactcgcg gtcgtcgccc ggtttttcga gcgcatcggc gatcatgcgg tcaacatcgg
      541 cgagcgtgtc cgctacgtgg tgaccgggtg gctgcccgaa caccgtggtg cagcccgtta
      601 ccgcagccgc gatgcggcca gcagcggtga tgccagcagc ggtgaggcca gcagtagcga
      661 tgccagcagt agcgatgctg ctgacgccac cgacgatcgg gccggcatgc cggtcgacgg
      721 gtgatgcgcg cgtgacgacc tggctggccg ctgtggtcgg catcggcatc ggcgtggtcg
      781 ccggtgccgg catcgccgtg ctggtgatgc gtggccgtgt cgcagcggca cccgacgccg
      841 gtcgtgacgg aagcgaactg gcgctgcacc aggcgcagcg tgtcgccgac cgattgcagg
      901 ctgcgctcga cgcgctgccc gtcggcgttg tggtgtccga tgcagccggg cgggtggtgg
      961 tgcgcaatcg ggctgcggac aactccggcg gcgacacgca caacgaggtg ttggtgcagg
     1021 ctgccgtgca gcatcacctg cgcggggcgt tgcgtggagg gccgcgctcg cagaccgtcg
     1081 agctgttcgg tccgccccgg cggatcgtgg tggtgcacgc ccgcccgctg gccggtgagg
     1141 gtgcggtggc caccatcgag gacatcaccg aacgctcgcg gctcgactcg gtgcgcaccg
     1201 acttcgtggc caacatcagc cacgagctca agacgccgat cggcgcgctg gcggtgttgg
     1261 ccgagaccat gatcgacgag cacgatcccg atctgatccg ccgtctcgcg ggcaagaccg
     1321 tcaacgaagc acaccgggcg gctggcatca tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnca acgtcgcgcc gatcgccgcc
     1501 gatctctcgc tcaacgccga tcagggtcag ctcgcgtcag ccctcggcaa cctggtcgac
     1561 aacgccgtga agtacagcga ggcgggcgac acggtcacca tcgccgccgt gcgcgagtcg
     1621 aacggggtga gcttcaccgt tgccgattcg ggcatcggca tccctgcccg tgacctcgat
     1681 cgcatcttcg aacgcttcta ccgcgtcgac cgggcgcgca gccgtggaac cggcgggacc
     1741 ggtctgggct tgtcgatcgt gcgtcacgtg gccaacaacc acggtggcgc ggtcacggtc
     1801 gcgtcgcgcg ggtcgctcag cgaccccgcc gggcgagctg aggcgatggc cctgatcgga
     1861 gcggcatgac ccacatcctc gggatcctca acgtgacccc cgactcattc agcgacggtg
     1921 ggcagttcga cgaggtggat gcggcgatcg cccgcgggcg gcagctgcgc gacgcggggg
     1981 ccgacctcgt ggacgtcggc ggggagtcga ctcgtccgnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gggctcgccg accccgacat gtaccgggtg
     2221 atcgccgaga cgaagctcac ctacatcgcc atgcactcgc gcggcccgag cgccgagacg
     2281 gccgcctacg aggacgtggt cgcggaggtg cgggccgacc tcaaggcgcg actcgccgag
     2341 atggtcgtcg tcggggtgga cctgaaccgg gtgatcctcg acccgggact cggcttcgcc
     2401 aaggac
//
LOCUS       DAORNF010000548         2400 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_33240_length_2400_cov_1.306610, whole genome shotgun sequence.
ACCESSION   DAORNF010000548 DAORNF010000000
VERSION     DAORNF010000548.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33240_length_2400_cov_1.306610"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    238..441
                     /estimated_length=204
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(471..>674)
                     /locus_tag="PLV68_17655"
     CDS             complement(471..>674)
                     /locus_tag="PLV68_17655"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRE03129.1"
                     /translation="TPLATGLSIMDDLIADVPELGPIFVNTMPVEITQPEDIADAVLF
                     LASDEAKYVTGLEMKIDAGCTIR"
     assembly_gap    675..733
                     /estimated_length=59
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<734..>1121)
                     /locus_tag="PLV68_17660"
     CDS             complement(<734..>1121)
                     /locus_tag="PLV68_17660"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRE03130.1"
                     /translation="DDLAETARMVESKGRAVVAVPADVRDVQSMADAVQQGVDRLGKI
                     DIVVANAGICTMQHWDQVSDQTWKDTIDVNLTGVWNTCKLTIPHLIANGGGSMILVSS
                     VAGLKGQPFLAPYVAAKHGVVGLMRTL"
     assembly_gap    1122..1134
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1400..1819
                     /locus_tag="PLV68_17665"
     CDS             1400..1819
                     /locus_tag="PLV68_17665"
                     /inference="COORDINATES: protein motif:HMM:NF024966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HRE03131.1"
                     /translation="MTTHSDLTDRLDIAEVLNRYAFAIDDKDWVALGEIFTDDCIADY
                     GPFGTFDGSAAVVDWMAPAHVGLTTQHAMANMVITVSGDTATARSYVTVSLQPDGHEP
                     FRVGGAYHDEFRRTGDRWQISNRSYTTIWQHGAPPGG"
     gene            complement(1842..>2400)
                     /locus_tag="PLV68_17670"
     CDS             complement(1842..>2400)
                     /locus_tag="PLV68_17670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRE03132.1"
                     /translation="RREFSGGQCQRISIARALALEPKMLICDEAVSALDVSVQAQILN
                     LLQEMKQRFNLTLIFVAHDLAVVKRVSDRVAVMYLGKIVEIAPADALYAEPRHHYTQA
                     LLTSIPQPDPSIRPEDVPLLGGEPPSPSNPPSGCRFRTRCPRAEALCAEVEPPLSSIG
                     PRHDVACHFPLDVAPARVAVRSSVA"
BASE COUNT          375 a          723 c          666 g          360 t
ORIGIN      
        1 cgcttctgca tctccggctc cagcacggcc acggcggcct tcatcgcctc ggcggccagc
       61 atcatatcgg gcaagaacat ctcacccgcc aacatcgagg cgcggttgaa gcagcaaccg
      121 ctcatcgccg aggcctgcgc gatcggcgat cggcgccact tcgtcaccgc ggtcctgacg
      181 ctcgacgccg atgcggcgac gaagtgggcg acggcgcacg ggcgcgaggg ggcgacannn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nactgatcgt tctcaccgct cggcatcagg tcagcggatc
      481 gtgcagccgg cgtcgatctt catctccagg ccggtgacgt acttcgcttc gtccgacgcc
      541 aggaagagca cagcgtcggc gatgtcctct ggctgggtga tctcgaccgg catggtattg
      601 acgaagatcg ggccgagttc ggggacatct gcgatcagat cgtccatgat gctgagtcct
      661 gtggccaaag gtgtnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      721 nnnnnnnnnn nnncgagcgt gcgcatcagg ccgacgacac cgtgcttggc cgccacgtac
      781 ggagcgagga agggctgtcc cttcaagcct gcgacggagc tgaccaagat catcgagccg
      841 cctccgttgg cgatgagatg tgggatcgtc agcttgcagg tgttccacac gcccgtcagg
      901 ttgacgtcga tggtgtcctt ccacgtctga tcactgacct gatcccagtg ctgcatggtg
      961 cagatgccag cgttggcgac gacgatgtcg atcttgccca gccgatcgac gccctgctgc
     1021 acggcgtctg ccatcgattg cacgtcgcgc acgtcggcgg gcactgcaac gacggcccgt
     1081 cccttggact cgaccatccg cgctgtctcg gcgaggtcgt cnnnnnnnnn nnnnccaggg
     1141 cgtacgtcac cgtgtcgatg ttctcgcaga tgtcggtgat gatgatgtcg gcaccctctt
     1201 cggccagacg caccgcctcg gcacgtcctt gacccagcgc tccgccggtg atgaacgcga
     1261 cctttccctc cacacgaccc atgtccgacg tccttccgaa caaccccggg tgatgcaacc
     1321 accccgggca acccctgtgg ccacccgcgc cgagtgtaca tgctctcgac caaccgcctg
     1381 tttgattagg atctggtgga tgacgacaca ctccgatctg accgaccgtc tcgatatcgc
     1441 cgaagtgctc aatcgctacg ccttcgcgat tgacgacaag gactgggtgg cgctcggcga
     1501 gatcttcacg gacgattgca tcgccgacta cggcccgttc ggcacgttcg acggctcggc
     1561 cgcagtcgtc gactggatgg ccccggccca cgtcgggctg accactcaac acgccatggc
     1621 caacatggtc atcaccgtgt ccggcgacac ggcgacagcc cggtcatacg tcaccgtcag
     1681 cctgcagccc gacggccacg aaccgttccg cgtcggtggc gcctaccacg acgagttccg
     1741 caggaccggc gaccggtggc agatctcgaa ccgctcctac accaccatct ggcagcacgg
     1801 ggcgccccca ggcggctgac gagccgaccg gtcagcgcgg ttcacgcgac cgacgatcgc
     1861 accgccactc gggcaggagc gacgtcgagt ggaaagtgac acgcgacgtc atggcgggga
     1921 ccgatcgagc tgagcggcgg ctcgacctcg gcgcagagtg cttcggcccg tgggcagcgc
     1981 gtcctgaaac ggcaccccga aggggggttg ctcggcgacg gcggctctcc gccgagcaac
     2041 gggacgtctt cgggccggat cgacggatcc ggctgtggaa tcgaagtcag cagtgcctgc
     2101 gtgtagtgat gacgcggctc ggcgtagagc gcatcggccg gcgcgatctc gacgatcttg
     2161 cccaggtaca tcaccgccac ccgatcactg acccgcttga ccaccgccag gtcgtgcgcc
     2221 acgaagatca acgtcaggtt gaaacgctgc ttcatctcct gcagcaggtt gagaatctgc
     2281 gcctgcacgg agacgtcgag agccgagacc gcttcgtcac agatcaacat cttcggctcg
     2341 agcgcgagcg cgcgggcgat cgagatgcgc tggcactggc caccggagaa ctcgcgcctg
//
LOCUS       DAORNF010000549         2398 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_33258_length_2398_cov_2.372599, whole genome shotgun sequence.
ACCESSION   DAORNF010000549 DAORNF010000000
VERSION     DAORNF010000549.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33258_length_2398_cov_2.372599"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="PLV68_17675"
     CDS             <1..325
                     /locus_tag="PLV68_17675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03133.1"
                     /translation="GSDGSSSTTLPPADLVIDAGPGLVWGQTAYTTTAGSAVAVAVVN
                     RDTILHTMVIVDDTKKVLGPELEVGKRGDVDTGTFNLAPGEYQILCRVPGHGGMTATL
                     TVTGE"
     gene            complement(352..1119)
                     /locus_tag="PLV68_17680"
     CDS             complement(352..1119)
                     /locus_tag="PLV68_17680"
                     /inference="COORDINATES: protein motif:HMM:NF017945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HRE03134.1"
                     /translation="MELLTRTGLAFGFRWLHVLFGITWIGLLYYFNMVQVPAFAAYGD
                     EGRARNIALHNVARRALWWFRWAAVFTLGTGLLILGVTEGYMKNFLSDSADGLPRGLT
                     GHDAVITVGMLFGITMAANVWMIIWKNQKVVLANAVNLLNGGEADPAAAGAGRAALLA
                     SRQNTIFSVAMLFFMVGAAHFYNSGAFAASSGAATTFVLVAIVIGAVLECLCVGLIGG
                     KAATNKLLWPYESHKNAIISAFVLWAVLWILSEIILG"
     gene            complement(1193..>2398)
                     /locus_tag="PLV68_17685"
     CDS             complement(1193..>2398)
                     /locus_tag="PLV68_17685"
                     /inference="COORDINATES: protein motif:HMM:NF022169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2510 domain-containing protein"
                     /protein_id="HRE03135.1"
                     /translation="GSSVRGVSMKPPPIPPALSQLRSMRARQMSALRQPTMRLPRGSV
                     ALQSQMVVAIMALGCATLAAIPFVNHYDLASNAARIATIIALAATVCGAVMTPLRFLA
                     GPPLVCGGVALFVANAITLAISRIDISSALDTAESKIVLIAGVAGLVAYVTAVPLLVG
                     RGLGSLTAVISLAAVAVVVATAFLVRIDDDQTWPQAGAIIAAVFVAAVIITGASKGRF
                     GSVATLVAAVVQLPGWIDLADDGAGDRRWLVFVALGATALIVVLAALTLVLALTGTAD
                     RAFGIGPRRAVVSPVVQPMVSPVSPVMVPAAHARHGGVLTPVSITLAAHDPAAGSSAM
                     PVGAVAHTSSTSASSGGVVVVTAAPVVAVPAVWSADPYGRHQLRYWDGAVWTEHVSDG
                     GVVTVDVMA"
BASE COUNT          456 a          834 c          785 g          323 t
ORIGIN      
        1 cggcagtgac ggttcgtcgt ccaccacgct gccgcctgcc gacctggtga tcgacgccgg
       61 ccccggcctg gtgtggggac agacggccta caccacaacc gctggctcgg ccgtggccgt
      121 cgcggtggtc aaccgcgaca ccatcctgca cacgatggtc atcgtggacg acaccaagaa
      181 ggtgctcggc cccgagttgg aggtgggcaa gcggggcgac gtcgacaccg gcacgttcaa
      241 cttggcaccg ggtgagtacc agatcctgtg ccgggttccc ggccacggcg gcatgaccgc
      301 cacgctgacc gtcaccggcg agtgacgatc agcgagccgt gactcccggt ctcagccgag
      361 gatgatctcg ctcaggatcc agagcacggc ccacagcacg aaggccgaga tgatcgcgtt
      421 cttgtggctc tcgtagggcc acagcagctt gttggtcgcc gccttgccgc cgatgaggcc
      481 gacgcacagg cattcgagca cggcaccgat cacgatcgcg accagcacga acgtggtcgc
      541 cgcgccgctc gatgcagcga aggcacccga gttgtaaaag tgggccgcgc cgaccatgaa
      601 gaacagcatc gccaccgaga agatggtgtt ctggcggctg gcgagcagcg ctgcccgtcc
      661 ggcgccagcg gccgcagggt cagcctcacc gccgttgagc aggttgaccg cgttggccaa
      721 cacgaccttc tggttcttcc agatgatcat ccacacgttg gccgccatgg tgatgccgaa
      781 cagcatgccg accgtgatca ccgcgtcgtg cccggtgagg ccacgcggta gtccgtcggc
      841 cgagtcggag aggaagttct tcatgtagcc ctcggtgaca ccgaggatca gcagaccggt
      901 gccgagcgtg aacaccgccg cccaacggaa ccaccacagg gcgcggcgcg cgacgttgtg
      961 gagggcgatg ttgcgcgccc gcccctcatc gccgtatgcc gcaaacgcgg gcacctgcac
     1021 catgttgaag tagtacagca agccgatcca cgtaatgccg aacagcacgt gcaaccaacg
     1081 gaacccgaag gccagaccag tacgcgtcaa cagttccatc tgccgttccc cttctttggc
     1141 aactgaatgt cgtgaaccta gcaggctcgt cagagcgcgc caatgatgct ggtcaggcca
     1201 ttacatcgac cgtcaccacg ccaccgtcgg agacgtgttc ggtccacacc gcgccgtccc
     1261 agtagcgcag ttgatggcga ccgtacgggt cggcgctcca gaccgctggc accgcgacga
     1321 caggggctgc cgtcaccacg acaacgccac ccgacgacgc agaggtggag gatgtatggg
     1381 caacggcgcc aaccggcatc gccgacgaac ctgccgccgg gtcgtgtgcg gcgagggtga
     1441 tgctgaccgg cgtgagcacg ccgccgtggc gggcgtgggc ggcgggcacc atcaccgggc
     1501 tcacggggct gaccattggt tgcaccaccg gcgagacgac ggcgcggcga gggccgatgc
     1561 cgaaggcccg atcggcagtc cccgtcagcg ccaacaccag cgtcagcgca gccagcacca
     1621 cgatcagcgc cgtggcgccc agagcgacga acaccagcca acgccggtcg ccggcaccgt
     1681 cgtcggccag gtcgatccaa cccggcagtt gcaccaccgc cgcgaccagc gtggcgaccg
     1741 atccgaagcg gcccttgctc gccccggtga tgatcaccgc ggcgacgaac acggcagcga
     1801 tgatcgctcc ggcctgaggc caggtctggt cgtcgtcgat gcgcaccaag aaggccgtgg
     1861 cgaccaccac ggcgacggcc gcgagcgaga tgacggctgt cagcgaaccg aggccgcgac
     1921 cgacgagaag cggcaccgcc gtcacatagg cgaccaggcc ggcgacgccc gcgatcagca
     1981 cgatcttcga ttcagcggta tcgagcgcgc tcgatatgtc gatgcgtgag atcgccagcg
     2041 tgatcgcgtt ggcgacgaag agggccacac cgccgcacac cagcggcggc ccggccagga
     2101 accgcagcgg cgtcatcacc gcgccgcaca ctgttgccgc caaggcgatg atcgtggcga
     2161 tgcgcgccgc gttggatgcg aggtcgtagt ggttgacgaa cgggatcgca gccagcgtgg
     2221 cgcaccccag cgccatgatc gccaccacca tctgcgactg cagcgccacc gacccacgcg
     2281 gcaaacgcat ggtcggctga cgcagcgcgg acatctgccg tgcccgcatc gaccgcagtt
     2341 gcgacaacgc aggcggaatc ggaggcggct tcatcgacac accgcgcacg ctactccc
//
LOCUS       DAORNF010000550         2397 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_33276_length_2397_cov_1.823228, whole genome shotgun sequence.
ACCESSION   DAORNF010000550 DAORNF010000000
VERSION     DAORNF010000550.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33276_length_2397_cov_1.823228"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>239)
                     /locus_tag="PLV68_17690"
     CDS             complement(<1..>239)
                     /locus_tag="PLV68_17690"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03136.1"
                     /translation="TVTITIFAGVVTTLLAAIVPAVASGRVSPVAAMNDAVLEPVRSM
                     RPRALTAAAFVAVGIASTIAVLAGADAILLGVAVV"
     assembly_gap    240..289
                     /estimated_length=50
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<290..1330)
                     /locus_tag="PLV68_17695"
     CDS             complement(<290..1330)
                     /locus_tag="PLV68_17695"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRE03137.1"
                     /translation="MFRLALRNITARFGRVVLTALAIVASTAFLSGTFIFRDTLQASF
                     DALFAKTYENVDVYVQSANSVETLFGFERRDATQFDAVAIAASAAGVDDAQASVIDDA
                     VVIAKDGTPIQRPSRAASGGTINSGPLSVWRIVSGRLPDGGTEVVLDTQTAQDGRYAL
                     GDTVKVNASGSRSFTLVGLAEYNDISTPGDSTWALFDEQTAMEFILRPGYIDAVLVRG
                     DGSVGDDVLAQRVQAALDAAQGPGVSEALTGAEIIEQSQTDLEKNLSFLTLFLSIFSL
                     IALGVGCFVIYNVFSITAAERRRENALLRAIGASRRQVTRSMLVEAVAIGLFGSLTGL
                     AANHRSFDVPLDR"
     assembly_gap    1454..1531
                     /estimated_length=78
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1532..>1944)
                     /locus_tag="PLV68_17700"
     CDS             complement(<1532..>1944)
                     /locus_tag="PLV68_17700"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HRE03138.1"
                     /translation="DSGQPLFDFFVLAQPAPGVSTADAVQAIDTVLDRYPTAKVQTRG
                     EFIDDQMAQLDGILNFIYALLLMSVFIAILGIVLTLLLAVYERRRELGLMRGIGATRG
                     QIRSSIRWEAVITSLFGVAIGLALGLFMGWIVVKA"
     assembly_gap    1945..1954
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1955..>2397)
                     /locus_tag="PLV68_17705"
     CDS             complement(<1955..>2397)
                     /locus_tag="PLV68_17705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03139.1"
                     /translation="LQSSLREVIGKAFTGEIAVRSKSDNGGILPTTLARDLATLDQVD
                     GVAGLGGALYFQTGDDGTRKGKQTIVTDPVEFAKVYHLDFDQGGWDGLGGNSVIVSQE
                     YADKDGLSLGSPIPITQLNGIELTLSVGGIFAEETFGDMIADRAA"
BASE COUNT          384 a          740 c          787 g          348 t
ORIGIN      
        1 cgaccacggc gaccccgagc aaaatcgcgt cggctccggc caacaccgcg atcgtcgacg
       61 cgatgccgac ggcgacgaac gcggctgcgg tgagtgcccg cggccgcatg ctgcgcaccg
      121 gttcgagcac cgcatcgttc atcgctgcga ccggtgacac ccgcccggac gcgacggcgg
      181 gcacgatcgc ggccagcaag gtggtgacga ccccggcgaa gatcgtgatc gtcaccgtcn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng cggtccagcg
      301 gcacgtcgaa gctgcggtgg ttggcggcca gcccggtcag cgaaccgaac agaccgatcg
      361 ccaccgcctc gaccaacatg ctgcgcgtca cctgacgacg gctcgctccg atcgcgcgca
      421 gcagcgcgtt ctcgcgccgc cgctcggccg cggtgatgga gaacacgttg tagatcacga
      481 agcagccgac cccgagcgcg atcagggaga agatcgacaa gaacagggtc aggaagctga
      541 ggttcttctc gaggtcggtc tggctctgtt cgatgatctc cgccccggtc agcgcctcgc
      601 tgacacctgg cccttgtgca gcgtcgagcg cggcctgcac ccgctgggcg agcacatcgt
      661 cgccgacgct gccgtcgccg cgcaccagca ccgcgtcgat gtagccgggg cggaggatga
      721 actccatcgc ggtctgttcg tcgaacagcg cccaggtcga atcgcccggc gtggagatgt
      781 cgttgtactc ggccaatccg accagggtga agctgcgcga gccggacgcg ttgaccttga
      841 cggtgtcgcc gagcgcgtag cgaccgtcct gcgctgtttg cgtgtcgagc accacctcgg
      901 tgccgccgtc gggcaaacgc ccgctgacga tgcgccacac cgacagcgga ccggagttga
      961 tcgtgccgcc ggacgcggcc cggctcgggc gttggatcgg tgtgccgtcc ttggcgatca
     1021 cgaccgcatc gtcgatgacc gaggcctgcg cgtcgtccac cccggccgcc gacgcggcga
     1081 tcgctaccgc gtcgaactgg gtggcgtcgc gccgttcgaa cccgaacagc gtctcgaccg
     1141 agttggccga ctggacgtag acgtcgacgt tctcgtacgt cttcgcgaac agcgcgtcga
     1201 agctggcctg gagcgtgtcg cggaagatga acgtgccgga caagaacgcg gtgctcgcca
     1261 cgatcgccag ggccgtcaac acgacccgcc cgaagcgggc tgtgatgttg cgcagagcga
     1321 gacgaaacat ggttccggtg gaggctaggc gagtgctcgg tcgtggcgag cgctacgtgg
     1381 tggcgatcgc ttcgaggatc ggcgccctgg cggcgcggcg ggcggggatc caggcggcgg
     1441 ccagcgtgag cagnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn naacgccttg accacgatcc agcccatgaa
     1561 cagtccgaga gcaagaccga tcgcgacgcc gaagagcgag gtgatcacgg cctcccagcg
     1621 gatgctgcta cgaatctggc cgcgggtggc accgatgccg cgcatcaggc cgagctcgcg
     1681 gcggcgctcg tagacggcga gcaacagcgt gagcacgatg ccgaggatgg caatgaacac
     1741 cgacatcagc agcaaggcgt agatgaagtt gaggatgccg tcgagttgag ccatctggtc
     1801 gtcgatgaac tcgccgcggg tctgcacctt ggcggtcggg tagcgatcga gcacggtgtc
     1861 gatcgcctgg accgcgtcgg cggtgctgac ccctggcgcc ggttgggcga gcacgaagaa
     1921 gtcgaacagc ggttggccgc tgtcnnnnnn nnnnaggctg cccggtcggc gatcatgtcg
     1981 ccgaaggtct cctcggcgaa gatgccgccg acggagagcg tcagctcgat gccgttgagc
     2041 tgtgtgatcg ggatcggcga gccgagcgac aggccatcct tgtcggcata ctcctgcgag
     2101 acgatcaccg agttgccacc gaggccgtcc cagcctcctt gatcgaagtc gaggtggtac
     2161 accttggcga attcgaccgg atcggtgacg atcgtctgct tgcccttgcg ggtaccgtcg
     2221 tcgccggtct ggaagtacag cgcgccgccg agcccggcta ccccgtcgac ctgatcgagg
     2281 gtggccaggt cgcgtgccag ggtggtcggc aggatgccac cgttgtcgga cttggaccgc
     2341 acggcgatct cgccggtgaa ggccttgccg atcacctctc gcaacgagct ctgcagc
//
LOCUS       DAORNF010000551         2395 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_33317_length_2395_cov_1.540598, whole genome shotgun sequence.
ACCESSION   DAORNF010000551 DAORNF010000000
VERSION     DAORNF010000551.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33317_length_2395_cov_1.540598"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>255
                     /locus_tag="PLV68_17710"
     CDS             <1..>255
                     /locus_tag="PLV68_17710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03140.1"
                     /translation="HRDERSVRVFSPAGLKAFMSVDGPLDLQNQDQVWFLEWANTSQT
                     GFNPSQVIYVFPFVGDSAPGSGSQRQTYGSEYAGTLQLTGP"
     assembly_gap    256..265
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <266..>808
                     /locus_tag="PLV68_17715"
     CDS             <266..>808
                     /locus_tag="PLV68_17715"
                     /inference="COORDINATES: protein motif:HMM:NF028519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HRE03141.1"
                     /translation="TARCLRRLANVRGQGGNDVAGTWLYTSAAPASIRQEPDAPSNNL
                     ISGATQWCAQADFGTAGCPASFAQVTGARFIQSGSMPGGTIITATVPLMANGNNPNDL
                     YVGKFGVFTPTFDNDDPAVIDALLSNEPYVMIVGFSLGDLVWLDTNNNGTYQSGVDTP
                     IANVPIEVLDSANTVVATATT"
     assembly_gap    809..867
                     /estimated_length=59
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            907..>1148
                     /locus_tag="PLV68_17720"
     CDS             907..>1148
                     /locus_tag="PLV68_17720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03142.1"
                     /translation="MTVGVVADPNDDPLAIPRRQVNENMDNNTLASGGGVLTQPVTLA
                     ADVEAVSGVITGLEPLGDDVAGLTPRLVGDGFTNYTV"
     assembly_gap    1149..1273
                     /estimated_length=125
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1283..>1540
                     /locus_tag="PLV68_17725"
     CDS             1283..>1540
                     /locus_tag="PLV68_17725"
                     /inference="COORDINATES: protein motif:HMM:NF039610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5979 domain-containing protein"
                     /protein_id="HRE03143.1"
                     /translation="MHDSTVQLNRDGTTVTISGLPTPSTCTVAELDTDGGRVSYNPAP
                     TVAITGGAATSLQVVNEFPNVVVHKEVSDLVETSIGQYVATY"
     assembly_gap    1541..1665
                     /estimated_length=125
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1666..2029
                     /locus_tag="PLV68_17730"
     CDS             <1666..2029
                     /locus_tag="PLV68_17730"
                     /inference="COORDINATES: protein motif:HMM:TIGR01167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HRE03144.1"
                     /translation="PTWNGLRDTEVVVDAAIDGLATHRYKVTVALSVGSKTTSEGADC
                     VLTDSETGTGLLNTVALTSNGLTSHADACRAPGIPPDQQVPKTGTDTLQPLAAGLVLM
                     VAGGFLLHASRRRRRVAT"
     assembly_gap    2040..2137
                     /estimated_length=98
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          332 a          692 c          642 g          312 t
ORIGIN      
        1 catcgtgacg agcgctcggt gcgggtgttc tctccggctg gcctcaaggc attcatgtcg
       61 gtcgacggcc cgctcgatct ccagaaccag gaccaggtgt ggttcctgga gtgggccaac
      121 acgtcgcaga cgggcttcaa cccgtcgcag gtcatctacg tgttcccatt tgtcggtgac
      181 agcgcgccag gcagtggctc acaacgccag acctacggca gcgagtacgc gggcacgctg
      241 caactgaccg ggccgnnnnn nnnnnactgc tcgatgccta cgccgcctcg ccaacgttcg
      301 cggccaaggc ggcaacgacg tcgccggtac gtggctgtac acctcggcgg cgccggcctc
      361 gatccgccag gaacccgacg cgccgtccaa caacctgatc agcggcgcca cgcagtggtg
      421 tgcacaggcc gacttcggta cggctggctg tccggcgagc ttcgcccagg tgacaggtgc
      481 ccggttcatc cagtcgggct cgatgcccgg cggcaccatc atcaccgcga ccgtgccgct
      541 catggccaac ggcaacaacc ccaacgatct gtacgtcggc aagttcggcg tgttcacacc
      601 gaccttcgac aacgacgacc cggccgtgat cgatgccctg ttgtcgaacg agccgtacgt
      661 gatgatcgtc ggattctcac tcggtgacct ggtgtggctc gacaccaaca acaacggcac
      721 ctaccaatcg ggcgtcgaca cgccgatcgc caacgtgccg atcgaggtac tcgattcggc
      781 caacaccgtg gtggcgaccg cgacgaccnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      841 nnnnnnnnnn nnnnnnnnnn nnnnnnngcc ttcgcggtgg gtggaccgct cttcggctac
      901 gggcctgtga ccgtcggtgt cgtcgccgac cccaacgacg acccgctcgc catcccccgc
      961 cgccaggtga acgagaacat ggacaacaac accctcgcca gcggcggcgg cgtgttgacc
     1021 cagcccgtga ccttggcggc agatgtcgag gcggtgtcgg gcgtgatcac cggcttggaa
     1081 ccgctcggcg acgacgtggc ggggttgacc ccgcgcctgg tcggtgacgg gttcaccaac
     1141 tacaccgtnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnggagtga ccgtgcacga ctcgacggtg caactcaacc gtgacggcac
     1321 gacggtgacg atcagcgggc tgcccacacc ctcgacgtgc accgtcgccg agctcgacac
     1381 cgacggcgga cgggtgagct acaacccggc gccgacggtg gcgatcaccg gtggtgcggc
     1441 cacctcgttg caggtggtca acgagttccc gaacgtcgtg gtgcacaagg aggtgtccga
     1501 cctggtcgag accagcatcg gccagtacgt ggcgacctac nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncccga cgtggaacgg
     1681 gttgcgcgac accgaagtcg tcgtcgatgc agcgatcgac ggtcttgcca cccaccgcta
     1741 caaggtgacc gtggcattgt ccgtcgggtc gaagacgacg tctgaagggg ccgactgcgt
     1801 gctcaccgac agtgaaacgg gcaccggctt gctcaacaca gtggcgctga cctccaacgg
     1861 tctgacgtcc cacgcagatg cctgccgcgc cccggggatc ccgccggacc agcaggtccc
     1921 caagacgggc accgacaccc tccagccgtt ggccgccggg cttgtcctga tggtcgccgg
     1981 tgggttcctg ttgcacgctt cgcgccgccg ccgacgggtt gccacgtgag ctggcgtcan
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncgg ggatgcgggt gggcgctgct
     2161 ggccgaccgt ccatggtcag cggcgactca aagcccttgg gggccagagc gagtcccgcc
     2221 cgtccctggc ccccaaggca tcgcacctgc gggtgcccga ccgcgtgctc gacgtgagcg
     2281 aggctcatct gccattcgag gtttgtgagc ccgaggtctc cgcccacacg gtgctcagag
     2341 tccttggccg ggccccgacc ctcgggcgat cgtcacgccg ggcggaaccc ggtca
//
LOCUS       DAORNF010000552         2392 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_33368_length_2392_cov_2.029097, whole genome shotgun sequence.
ACCESSION   DAORNF010000552 DAORNF010000000
VERSION     DAORNF010000552.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2392
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33368_length_2392_cov_2.029097"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..572
                     /gene="nth"
                     /locus_tag="PLV68_17735"
     CDS             <1..572
                     /gene="nth"
                     /locus_tag="PLV68_17735"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HRE03145.1"
                     /translation="VLLSAQCTDARVNTVTPALFALADHPAAMATVPVESIREIIKPC
                     GLSPRKSAAISELSKILVARHGGEVPAEFEALEALPGVGHKTASVVMSQAFGVPAFPV
                     DTHIHRLAKRWGLSDGSTVERTERDLKAAFAIDTWNRRHLQIIFFGREYCPALRHDMM
                     LCPICSFAASKKQIAAELAPSTKARQRAT"
     gene            complement(557..1762)
                     /locus_tag="PLV68_17740"
     CDS             complement(557..1762)
                     /locus_tag="PLV68_17740"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HRE03146.1"
                     /translation="MSARRVRGAALVLTLALAAPACTSTTNAATNTPANTGASTANDT
                     SGDAAGGNTAANDDTSTALTTTTLAETTTTPAETSTTLPETTTTPESTTTTEPPLDVY
                     DVACVVEVRRGQSLAEIASAIPDEIVLPETIRDENGLADDVIHPDQLLDVCVGNGIDD
                     ITGEQRQSENEAVVSIQRHAVVVAQQEKLNELFAPYAISPLLVDGVSGPVTRQRLCAF
                     RLSYGLTVSTENMAGGSDEEIALMEATELKLPFTTSLLSPRWALIDRTCQIMFVGEGA
                     DRLVFVFPTSTGEPGYETRNQDRSRAFRFNPAVDNGGWHNSSTYPVAEDNPLNGNMYK
                     PIYFDNGQAIHGALNVPTAPRSKGCARLRVEHMNLLVQWLGLDTITYGVGAKQINLTV
                     NVQGTYVAR"
     gene            1992..>2392
                     /locus_tag="PLV68_17745"
     CDS             1992..>2392
                     /locus_tag="PLV68_17745"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HRE03147.1"
                     /translation="MTIELPPLPYAIDALAPHVSQETLEYHHGKHHQAYVTNLNGLIK
                     DTDLADKSLEEIILAAEPGPLFNNAAQVWNHTFYWNSMSPTGGGKPSGELADAIDSAF
                     GSYDEFSSQFSAAAAGQFGSGWAWLVQDDSGL"
BASE COUNT          371 a          786 c          803 g          432 t
ORIGIN      
        1 ccgtgctcct ttcggcgcag tgcaccgacg cacgcgtcaa caccgtcacg cccgctcttt
       61 tcgccctcgc cgaccacccc gccgcgatgg ccacggtgcc cgtcgaatcc atccgcgaga
      121 tcatcaaacc ctgtggtctt tccccgcgca agtcggccgc catttccgaa ctctcgaaaa
      181 tcctcgtcgc tcgacatggt ggcgaggtgc ccgccgaatt cgaggcgctc gaagccctcc
      241 ccggagtggg gcacaagacc gcctcggtcg tgatgagcca agccttcggt gtgcccgcct
      301 ttcctgtcga cacccacatc caccggctgg ccaagcgctg gggtctgtcc gacggcagca
      361 ccgtcgagcg caccgaacgc gacctgaagg ccgcgttcgc gatcgacaca tggaaccggc
      421 gccacctcca gatcatcttc ttcggccgcg agtactgccc ggcgctacgc cacgacatga
      481 tgctgtgccc gatctgctcg ttcgcggcga gcaagaagca aatcgccgcc gagctcgccc
      541 cgagcactaa ggcccgtcaa cgcgccacgt aggtgccttg gacgttgacc gtgaggttga
      601 tctgcttggc gccgacgccg taggtgatgg tgtcgagccc gagccattgc accaacaggt
      661 tcatgtgctc gacccgcagg cgggcacaac ctttgctgcg cggcgcggtg ggcacgttga
      721 gcgccccgtg gattgcctgt ccgttgtcga agtagatcgg cttgtacatg ttgccgttga
      781 gcgggttgtc ctcggcgacg ggataggtcg aactgttgtg ccaaccaccg ttgtcgacgg
      841 ccgggttgaa gcggaacgcc cgactgcggt cttggttgcg ggtctcgtag ccgggctcgc
      901 cggtggatgt gggaaacacg aagaccagcc gatcggcgcc ctcgccgacg aacatgatct
      961 ggcaggtgcg gtcgatcagc gcccaccgcg gcgagagcag cgacgtcgtg aacggcagct
     1021 tgagctcggt ggcctccatc agcgcgatct cttcgtcact gccgccggcc atgttctcgg
     1081 tcgataccgt gagcccatac gacaggcgga aggcgcacag ccgctggcgg gtgaccggtc
     1141 cggagacgcc gtcgaccagc agcggcgaga tcgcgtaggg ggcgaacagt tcgttcagct
     1201 tctcctgctg ggcgacgacc acggcgtggc gttggatcga tacgaccgct tcgttctcgg
     1261 actggcgctg ctcgccggtg atgtcgtcga tgccgttgcc gacgcacacg tcgagcagtt
     1321 gatccgggtg gatgacgtcg tccgcgaggc cgttctcgtc gcggatggtc tccggcaaca
     1381 cgatctcgtc ggggatcgcg gacgcgatct cggccaggga ctggccgcgg cggacctcga
     1441 cgacacaggc cacgtcgtac acgtcgagcg gcggctcggt ggtggtggtc gactccggcg
     1501 tcgtggtcgt ctcgggcagt gtggtgctgg tctcggccgg cgtggtcgtg gtctcggcca
     1561 gcgtcgtggt ggtgagcgcg gtcgaggtgt cgtcgttggc ggccgtgttc ccacctgcag
     1621 cgtcgccgct cgtgtcgttc gccgtgctgg cgccggtgtt ggccggggtg ttggtggcgg
     1681 cgttggtggt ggaggtgcag gctggcgcgg cgagggccag cgtcagcacg agcgcggcgc
     1741 cgcggacgcg ccgcgcggac acatctcggg cagcatcgcc tctcgccaac atcgccgcag
     1801 aaggtagtag ccctcgccgc gcgagtgggc gcgggtgcga tgtgaccgac atcacgttga
     1861 ggtccttgat ccagcgggaa ggggaagggt tagcgtcaat ttcgcaagca tttgcttgtg
     1921 tatctaccca caaccgtccg cggccacgtc gatcgacctg gtccgcgagc ccgcccactt
     1981 cggagctgat catgaccatc gaactgccgc cgctgcctta tgcgatcgat gcccttgcgc
     2041 ctcatgtctc gcaggagacc ctcgagtacc accacggcaa gcaccaccag gcgtacgtca
     2101 ccaacctcaa cgggttgatc aaggacaccg atctggccga caagtcgctg gaggagatca
     2161 tcctcgcggc cgagccaggg ccgctgttca acaacgctgc gcaggtgtgg aaccacacgt
     2221 tctactggaa ctcgatgagc cccactggcg gcggcaagcc ctccggtgaa ctggccgacg
     2281 cgatcgactc ggccttcggc tcgtacgacg agttctcctc gcagttcagc gcggcggcgg
     2341 ccggtcagtt cggctcgggt tgggcctggc tggtgcagga cgacagcggc ct
//
LOCUS       DAORNF010000553         2388 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_33434_length_2388_cov_1.468924, whole genome shotgun sequence.
ACCESSION   DAORNF010000553 DAORNF010000000
VERSION     DAORNF010000553.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2388
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33434_length_2388_cov_1.468924"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            24..>875
                     /locus_tag="PLV68_17750"
     CDS             24..>875
                     /locus_tag="PLV68_17750"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HRE03148.1"
                     /translation="MIREQIPDALGGERLDRIVALLTDASRSDAAALVAAGGASVNGS
                     VVVSGKVRVAVGDVVEIDPSELPSKALPVGDASIEVAVLYADDDVIVVDKPSGLVVHP
                     GHGHPDGTLVNALLARFPDMAEVGDPFRPGIVHRLDVGTSGVMVVARSAVAYDSLVAA
                     LSSRSVARTYNTLVWGHLDSPKGLIDAPIGRDHRDPLRMAVVIDGKPARTRYRTVETY
                     VDPTEVALLECQLETGRTHQIRVHLAAAGHPVVGDGQYGGQRSAITAHRPFLHAASLG
                     FTHPVTGQ"
     assembly_gap    876..897
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(968..1429)
                     /locus_tag="PLV68_17755"
     CDS             complement(968..1429)
                     /locus_tag="PLV68_17755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HRE03149.1"
                     /translation="MRISTKGDYACRALLSLALHADEPGPTSVADIAKRTGLPQPYLE
                     QILLALKGAGLVRSKRGVGGGYTLARPAEQIHLSEILSAVDGPISLGDFGEPHEDGAC
                     NHEGQCVLLAIWKDAGEMMRRHMEGFTLAEVARVAQGDRPWSDISRALHSG"
     gene            1609..>2006
                     /locus_tag="PLV68_17760"
     CDS             1609..>2006
                     /locus_tag="PLV68_17760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HRE03150.1"
                     /translation="MNDQPVLPTYMGPNIRGLIPALVGPTYAPLPSWMPSLAAAPRRV
                     LLVVDGLGWDQLTERWALAPTLASMEATTITTVAPSTTATALTSITTGLTPGEHGLIG
                     YRIDVGRDVLNVLRWRNESADLRRVHQPRSL"
     assembly_gap    2007..2026
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2027..>2388
                     /locus_tag="PLV68_17765"
     CDS             <2027..>2388
                     /locus_tag="PLV68_17765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HRE03151.1"
                     /translation="PPVISKAELETSAFTEAHLRGGRPYGWRVASAIAVEAARHIDAG
                     EPFVYAYYDGIDKTAHERGFGPFYDAELVAVDQLVAGVLARLPADATLLITADHGQVQ
                     VGDAIVQPDRSMMQMVRG"
BASE COUNT          341 a          809 c          807 g          389 t
ORIGIN      
        1 cagtccggtg acgaggtcgt ggcgtgatcc gtgagcagat tcccgacgcg ctcggcggtg
       61 agcgactcga ccgcatcgtc gcgctgttga ccgacgccag tcgatccgac gcggccgcgc
      121 tcgtggccgc cggtggcgcc tcggtcaacg gctcggtcgt cgtgtcgggc aaggtgcgcg
      181 tggcggtggg cgatgtcgtc gagatcgatc cgagcgagct tccgtccaag gcgctgcccg
      241 tcggtgacgc gtcgatcgag gtcgcggtgc tgtacgccga cgatgacgtg atcgtcgtcg
      301 acaagccctc ggggctcgtc gtccaccccg gtcacgggca cccggacggc acgctggtca
      361 atgcgttgtt ggcccgcttt ccggacatgg ccgaggtcgg tgacccgttc cgtcctggca
      421 tcgtccaccg tctcgacgtc ggcaccagcg gtgtgatggt ggtcgcccgc agcgcggttg
      481 cgtacgactc gctcgttgcc gcgctgtcgt cgcgctcggt ggcgcgtacg tacaacacct
      541 tggtgtgggg ccacctcgac tcgcccaaag ggctgatcga tgcgccgatc gggcgcgacc
      601 accgcgaccc gctgcgaatg gcggtggtga tcgacggcaa gccagcccgt acccgctacc
      661 gcaccgtcga gacgtatgtc gacccgaccg aggttgccct actggagtgc cagttggaga
      721 cgggccgcac gcaccagatc cgggtccacc ttgccgccgc cggccatccg gtcgtcggcg
      781 acggacagta cgggggccag cgcagcgcga tcaccgcgca ccgtccgttc ttgcacgccg
      841 cgtcgctggg gttcacccat cccgtcaccg gccagnnnnn nnnnnnnnnn nnnnnnnaga
      901 cgaactgctg gcgatcctcg ccgtactcgc gtgacgtgat cagcccgagt ggctgatcga
      961 cccgggatca acctgaatgc agagcgcggc tgatgtcgga ccacggccgg tcgccctggg
     1021 cgacccgagc cacctcggcc agcgtgaagc cctccatgtg gcggcgcatc atctcgcccg
     1081 cgtccttcca gatcgccagc agcacgcact ggccctcgtg gttgcaggcg ccgtcttcgt
     1141 gtggctcgcc gaagtcgccg agcgagatcg ggccgtcgac ggcggacaag atctcggaga
     1201 gatggatctg ctcggccggg cgggccagcg tgtaaccacc accgacgccg cgcttggagc
     1261 gcaccaggcc agcacccttc aacgccagca gaatctgctc gagatagggc tgaggcagac
     1321 ccgtgcgctt ggcgatgtcg gccaccgacg tcggtccggg ctcgtcggcg tgcagcgcca
     1381 acgacaggag agctcggcag gcgtagtcgc ccttggtgga gatgcgcacg gtgccagcgt
     1441 acctgtggac atctgccgcg atgcggccag gcggcaacgc cgatgcgatc gagcgtcggc
     1501 tgatcgtgac ggtgcccgat cgcggcaccc cctgaatcgt ggccgcggcc gacgtggcag
     1561 cggccgatcg tggcattcac agtccgaacg tggtggactg ttgccgccgt gaacgaccaa
     1621 cctgtgctcc ccacgtacat gggcccgaac attcgcgggc tgatccccgc actcgtcggt
     1681 ccgacgtacg ccccgctgcc gtcgtggatg ccatctctgg ctgctgcgcc gcgccgggtg
     1741 ctgctcgtgg tcgatggtct ggggtgggac cagctcaccg agcggtgggc gctcgcaccc
     1801 accctggcct cgatggaggc aaccacgatc accacggtcg cgccgagcac cacggccact
     1861 gcgctgacgt cgatcaccac gggtctcact ccaggcgagc atggcctgat cgggtaccgc
     1921 atcgatgtcg gccgcgacgt gctcaacgtg ttgcgttggc gcaacgagtc ggccgacctg
     1981 cgccgggtcc accagccgcg gtcgctnnnn nnnnnnnnnn nnnnnncccg ccggtgatct
     2041 cgaaggccga gttggagacg tcggccttca ccgaagccca tctgcgcggc ggtcgtccct
     2101 acgggtggcg ggtcgcgtcg gcgatcgcgg tcgaggccgc gcgtcacatc gacgccgggg
     2161 agccgttcgt gtacgcctac tacgacggca tcgacaagac ggcccacgag cgtggtttcg
     2221 gtccgttcta cgacgccgaa ctggtcgccg tcgaccaact tgtcgcgggc gtgttggccc
     2281 gtttgcccgc cgacgccacg ttgttgatca cggctgatca tggccaggtc caggtgggcg
     2341 acgcgatcgt ccagcctgat cggtcgatga tgcagatggt gcgcggcc
//
LOCUS       DAORNF010000554         2383 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_33528_length_2383_cov_1.366409, whole genome shotgun sequence.
ACCESSION   DAORNF010000554 DAORNF010000000
VERSION     DAORNF010000554.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33528_length_2383_cov_1.366409"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1327)
                     /locus_tag="PLV68_17770"
     CDS             complement(<1..1327)
                     /locus_tag="PLV68_17770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03152.1"
                     /translation="MTSVNISVVGEIATAFGIDNSAGQTIYDQTSTPVLVDPQVVVSG
                     GGTLDGAKVTITDGFLVGEDELSCAPMHGITCDYNATTGVLTLTGNATPAQYQAILRT
                     VLYDNSGVSPSGGTRTIQFSLGAAGLYFEPNGHFYEFVVANGITWSAARDAAAARRYF
                     GLQGYLATVTDETENSFIAAKLEGQGWMGASDAAAEGTWRWVTGPEGLENAGAGRHFW
                     SGTATGGPVGGFYNNWASGEPNNCCAGENHAHFLGNGSWNDYAFNNGSIQGYVVEYGG
                     LGGDPVIEVVDTKELLVVTCFDGLKNGVETDADCGGVCGSCGPGGACGGPADCESGGN
                     ICQLPACNDGVKNGLETGTDCGGPTCGDCGIGGGCFVGTDCTTGVCINDVCQAPTCND
                     GVSNGLETGTDCGGPVCGDCGPGEGCIGASDCSSGVCAGGACQAPSCFDE"
     gene            1401..>1945
                     /locus_tag="PLV68_17775"
     CDS             1401..>1945
                     /locus_tag="PLV68_17775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03153.1"
                     /translation="MVQLLPLGTVAPEHVLAMIENGAASGFGLPTLPTPTELPLLVSV
                     TTAVCEPPGLKRPKSMAVGVMVQLGWKTRMVPKAEPKAACAWLVACSVMLAMLSTAAP
                     AGTSTATSTEVCVRPARVIWVSPSSDVVHPTLLEVARSSVIGSGLRLWTMKGRVVRVP
                     GGLSCWGGAMTCTALGSVQVSV"
     assembly_gap    1946..2016
                     /estimated_length=71
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2017..2358)
                     /locus_tag="PLV68_17780"
     CDS             complement(<2017..2358)
                     /locus_tag="PLV68_17780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03154.1"
                     /translation="MAANSSVTLRRIITYTMGGYAYRMRFEVVNGGGSALSDVRLIHG
                     GDTYFGNDDSARSWWNADQNMVYVNNSSFRTSGIMGFAGSPATPAARFFSGNYSTGNT
                     QAQSGQLDNTSN"
BASE COUNT          328 a          702 c          888 g          394 t
ORIGIN      
        1 cctcgtcgaa acagctcggg gcctggcagg cgccgcccgc gcagacaccg ctcgagcagt
       61 cgctcgcgcc gatacagccc tcgcccgggc cgcagtcgcc gcagacggga ccgccgcaat
      121 cggtgccggt ctcgaggccg ttgctcacgc cgtcgttgca ggtcggggcc tggcagacgt
      181 cgttgataca cacgccggtc gtgcagtcgg tgccgacaaa acagccgccg ccgattccgc
      241 agtcgccgca ggtcgggcca ccgcagtcgg tgccggtctc gaggccgttc ttgacgccgt
      301 cgttgcaggc ggggagctga cagatgttgc cgccgctctc gcagtcggcc gggccgccac
      361 aggcgccacc cgggccgcag ctaccgcaga cgccgccgca gtccgcgtcg gtctcgacgc
      421 cgtttttcag gccgtcgaaa caggtcacga cgaggagctc tttcgtgtcg acgacctcga
      481 tgacggggtc gccgccaagg ccgccgtact cgacgacgta gccctggatc gagccgttgt
      541 tgaaggcgta gtcgttccac gagccgttac cgaggaagtg ggcgtggttc tcgccggcgc
      601 agcagttgtt gggctcgccg gaggcccagt tgttgtagaa accgccgacg gggccgcccg
      661 tcgcggtgcc ggaccagaag tggcgtccgg cgcccgcgtt ttccaggccc tcggggccgg
      721 tcacccagcg ccaggtccct tcggcggcgg cgtccgaggc gcccatccag ccctgaccct
      781 cgagcttggc ggcgatgaac gagttttccg tctcgtcggt gaccgtcgcg aggtagccct
      841 gcaggccgaa gtagcggcgg gccgcggccg cgtcgcgggc ggccgaccag gtgatgccgt
      901 ttgcgacgac gaactcgtag aagtggccgt tgggctcgaa gtagagaccg gcggcgccga
      961 gcgagaactg gatggtgcgg gtgccgcccg acggcgagac gccgctgttg tcgtacagaa
     1021 cggtgcgcag gatggcctgg tactgggccg gcgtcgcgtt gccggtcagc gtgagcacgc
     1081 cggtggtcgc gttgtagtcg caggtgatgc cgtgcatcgg ggcgcaggag agttcgtcct
     1141 cgccgacgag gaagccgtcg gtgatggtga ccttggcgcc atcgagggtg ccgccgccgg
     1201 agacgacgac ctgcgggtcg acgagcaccg gggtgctggt ctggtcgtag atggtctggc
     1261 cagcgctgtt gtcgatgcca aacgccgtcg cgatctcgcc gacgacgctg atgttgacgg
     1321 aggtcacggt cggggccgag agcgggatgt tgaacgagtc ggcgtagttg ccatcggtcg
     1381 agctcgtgaa gcggacggtg atggtgcagc tgctgccgct gggcacggtc gcgccggagc
     1441 acgtgttggc gatgatcgag aacggcgccg cgagcgggtt cgggctgccg acgttgccga
     1501 cgccgacgga gctgccgttg ttggtgagcg tcacgaccgc ggtctgcgag ccgccggggc
     1561 tcaagcggcc gaagtcgatg gccgtgggcg tgatggtcca gttgggctgg aagacgcgga
     1621 tggtgccaaa ggccgagccg aaggcggcct gcgcctggtt ggtcgcctgc agcgtgatgt
     1681 tggcgatgct gtcgacggcg gcgccggcgg gcacgtcgac ggccacctcg acggaggtct
     1741 gcgtgaggcc ggcgagggtg atctgggtgt caccgagcag cgacgtcgtc cacccgacgt
     1801 tgctcgaggt ggcgaggtcg agcgtgatcg ggtccgggct caggttgtgg acgatgaagg
     1861 gaagggtcgt gcgtgtgccc ggcgggctga gctgttgggg aggtgcaatg acctgcaccg
     1921 cgctcgggtc ggtccaggtc tcggtnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngttg ctggtgttgt cgagctggcc
     2041 ggactgggcc tgggtgttgc cggtcgagta gttgcccgag aagaaacggg cggcgggtgt
     2101 ggcgggcgag ccggcaaaac ccatgatgcc cgaggtgcgg aacgaggagt tgttgacgta
     2161 gaccatgttc tggtccgcgt tccaccagct ccgggcgctg tcgtcgttgc cgaagtaggt
     2221 gtcgccgccg tggatgaggc gtacgtccga gagcgcgctc ccgcctccgt tgacgacttc
     2281 gaaacgcatc cgataggcgt agccgcccat cgtgtaggtg atgatgcggc gcagtgtcac
     2341 gctgctgttg gctgccatgg tggtcgtgac ggtcaccgtc tca
//
LOCUS       DAORNF010000555         2382 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_33551_length_2382_cov_1.315428, whole genome shotgun sequence.
ACCESSION   DAORNF010000555 DAORNF010000000
VERSION     DAORNF010000555.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2382
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33551_length_2382_cov_1.315428"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>672
                     /locus_tag="PLV68_17785"
     CDS             <1..>672
                     /locus_tag="PLV68_17785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRE03155.1"
                     /translation="PPKVFPTSMTVVGSGVTGVEFVHMFSSFGADVTLVVSRQQVLPN
                     KDPEVAAVLEEDFLRRGVRLLKGARATAIERVDDQVVVSCDDGRVVRSSHAVLAIGSV
                     PNTDGLGLEAAGVRLDGGYVTVNRHCQSNVGHIYAAGDVSGKLPLSSVASMQGRKVAE
                     HVMGLQKVAHRHLDYDKAASAIFTEPEIADVGLAETEAFAEGRKIRVTKVPFSANPKA
                     LINNDS"
     assembly_gap    673..729
                     /estimated_length=57
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            777..872
                     /locus_tag="PLV68_17790"
     CDS             777..872
                     /locus_tag="PLV68_17790"
                     /inference="COORDINATES: protein motif:HMM:NF014860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03156.1"
                     /translation="MAVTARLRVSDIVESLLVHPALSEALAEAAE"
     assembly_gap    1054..1257
                     /estimated_length=204
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1258..1717)
                     /locus_tag="PLV68_17795"
     CDS             complement(<1258..1717)
                     /locus_tag="PLV68_17795"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:NF015084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HRE03157.1"
                     /translation="MSTTWHVSRVAVTGSTNDDLLAAGRDGAAHGTVLMADLQTAGRG
                     RLDRRWDAPAGANLLVSLLLRDLGAAPHEAVRRVALAARAAAETAGQTAHGASEIVLK
                     WPNDLLVRGADDSAAKLAGILAQLGHSSTGPFVVVGVGMNIGWGPEGAARL"
     gene            1812..>2382
                     /locus_tag="PLV68_17800"
     CDS             1812..>2382
                     /locus_tag="PLV68_17800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HRE03158.1"
                     /translation="MLKKILIANRGEIAVRVIRAARELGIQTVAVYSELDRNALHVRL
                     ADEAFALGGQTAAESYLNTEAIIDAIKQSGADGVHPGYGFFSENADFIRAISELGVAF
                     IGPPASAVDVMGDKVSSRKAALEGGAPIVPGTTDFAQSSQEITDFGTANGWPVAIKAA
                     FGGGGRGMKVVHSADEVESAMESAQREAKA"
BASE COUNT          346 a          748 c          718 g          309 t
ORIGIN      
        1 ccgccgaagg tgttccccac cagcatgacc gtggtcggct caggcgtgac cggggtcgaa
       61 ttcgtgcaca tgttctccag cttcggggcc gacgtcaccc tggtggtcag ccgccagcag
      121 gtgctgccca acaaggaccc cgaggtcgcc gcggtgttgg aagaagactt cctccgtcgg
      181 ggtgtgcgcc tgctgaaagg ggcgcgggcg accgcgatcg agcgggtcga cgatcaggtc
      241 gtggtcagct gcgacgacgg gcgcgtcgtg cgcagcagcc acgccgtgct ggcgatcggc
      301 tcggtaccca acaccgacgg actcggcctg gaggccgcgg gcgtgaggct cgacggcggg
      361 tacgtcaccg tcaaccgcca ctgtcagagc aacgtcgggc acatctacgc cgctggcgac
      421 gtcagcggca agctgccgtt gtcgtcggtg gccagcatgc agggccgcaa ggtggccgag
      481 cacgtgatgg gcctgcagaa ggtggcccac cgccatctcg actatgacaa ggctgcctcg
      541 gcgatcttca ccgaacccga gatcgccgac gtcggcctgg ccgagaccga ggccttcgcc
      601 gagggccgca agatccgggt caccaaggtg cccttctcgg ccaacccgaa ggcgttgatc
      661 aacaacgact ccnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      721 nnnnnnnnng ctcgatcgtc ggtcgtcacg cggccgagct gatcagcgtc atcgccctgg
      781 ccgtcaccgc ccgcctgcgg gtcagcgaca tcgtcgaaag cctgctggtc cacccggccc
      841 tgtccgaagc cttggccgag gctgccgaat agcccgaatc atccaaacgg cgatgtgtgt
      901 tcgatccacc ccgtgtggcg gggtcgaccg aacaccgttg ccggtcgagt gccgaagtgt
      961 ctccgatcca cccctcgtag cggggccaac tgaacaccgt tgccgggcgg gggtcagcgg
     1021 gggcggaggt ggacgacgtc gccggtgtcg atgnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncca
     1261 gacgggccgc cccctcgggc ccccagccga tgttcatgcc cacgcccacg accacgaacg
     1321 gcccggtcga gctgtggccg agctgggcga ggatgcccgc cagcttggcg gcggagtcgt
     1381 ccgccccgcg caccaacagg tcgttgggcc acttcagcac gatctcgctc gcgccgtggg
     1441 ccgtctggcc ggccgtctcg gccgcggcgc gcgccgccaa ggccacccgc cgcaccgcct
     1501 cgtgtggggc agcaccgagg tcgcgtagca gcaggctgac caacaggttg gccccggccg
     1561 gagcatccca gcggcgatcg aggcgccccc ggccagcggt ctgcagatcg gccatcaaca
     1621 cggtgccgtg cgcggccccg tcgcggcccg cagcgagcag gtcgtcgttg gtcgaaccgg
     1681 tcacggctac ccggctgacg tgccatgtcg tgctcatcgg gctcacgcta cgagcgacgt
     1741 gaccgaagct cacaggcgtc ccggccggag ttggttcgca tggcaccccc tgcagcgggc
     1801 atcatggcga cgtgctgaag aagatcttga tcgccaaccg tggcgaaatc gccgtccgcg
     1861 tcatccgagc tgcccgcgaa ctgggcatcc agaccgttgc ggtgtactcc gaactcgacc
     1921 gcaacgccct ccacgtacgc ctcgccgacg aggccttcgc cctcggcggc cagacagcgg
     1981 ccgagagcta cctcaatacc gaggcgatca tcgacgcgat caagcagagc ggtgccgacg
     2041 gcgtacaccc cggttacggc ttcttcagcg agaacgccga cttcatccgg gccatctccg
     2101 aactcggcgt cgccttcatc ggccctccag cgtcggcggt cgacgtcatg ggcgacaagg
     2161 tcagcagccg caaggcggcc ctcgaaggtg gcgccccgat cgtgccgggc acgaccgact
     2221 tcgctcagag ttcgcaggag atcaccgact tcggcaccgc caacggctgg cccgttgcga
     2281 tcaaggccgc gtttggcggc ggtggccgtg gcatgaaggt ggtccacagc gccgacgagg
     2341 tcgaatcggc gatggagagc gcgcagcgcg aggccaaggc ct
//
LOCUS       DAORNF010000556         2380 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_33577_length_2380_cov_1.668817, whole genome shotgun sequence.
ACCESSION   DAORNF010000556 DAORNF010000000
VERSION     DAORNF010000556.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33577_length_2380_cov_1.668817"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1609)
                     /locus_tag="PLV68_17805"
     CDS             complement(<1..1609)
                     /locus_tag="PLV68_17805"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRE03159.1"
                     /translation="MPESLPVLKDWKWELSYNFGRTQATQLNEGNLIVSRLGNALGPS
                     FFDSDGIARCGTSDAPIDGCVPMNVFAGADANAITQEMIDYVTFTGVSTGYNRQQTVL
                     GTMHGRLARTPWGGDIALAIGADHRREGGGYTPDPLTSTGDTTGNAQEPTDGSYNVTE
                     GFAELSAVPVVGQGLVQWLELNLAARAFDYSNFGSGITGKVGGLFRTSAGLAIRGSYS
                     TAFRAPNVGELFSGNADNFPQVDDPCDAQPGGADQPRTLPPEVQARCSAEGIPVGTIF
                     GLSQQRSQVGGNPNLTEETANVLTAGLVFEPPMVKGLAMTLDYFNVDITDTIQAQGAG
                     VILADCYTRGNQEACDRIKRNPVNFQIDSINDTVANVGGTRTSGIDFSVAYDRQFPFG
                     RLRTSLEGTYLLGFEFDNLVDPDKPLQAQGNYDFGVFPAIKTNFTTQWAKDGISAGFN
                     IRFVGSYDECENSDCNGLQADDPNGDGVDDGLAGIGIAVVARRHRRPFQHQLSFLARP
                     QKAVGVVNDAGIDVEVGAADAARLADSV"
     gene            complement(1507..>1977)
                     /locus_tag="PLV68_17810"
     CDS             complement(1507..>1977)
                     /locus_tag="PLV68_17810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03160.1"
                     /translation="NIRVCRRDGNGYAAFSAPADLYNYQPENYLYTPSERYNVFSTGS
                     YKVTDKIRTFFEGSYINRKSDQRLAPEPLFTASAGIAISADSVYNPIGENTAAASWSS
                     APAARSRTSTPSASWLASMARCPSRCPSSRTGSGSCPTTSAARRRPSSTRVTSS"
     assembly_gap    1978..1999
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2000..>2380)
                     /locus_tag="PLV68_17815"
     CDS             complement(<2000..>2380)
                     /locus_tag="PLV68_17815"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HRE03161.1"
                     /translation="IPLAVIERVEVLKDGASAIYGSDAIGGVVNIITRQDLNGSEATI
                     YTGTTTRGDGFGYDLSLVTGTSSKKGNVLFAAGYQSQEPVFAGDREFSNVDKQYDYEG
                     KMSVDIGSSAIPAGRFTHSGRQNI"
BASE COUNT          394 a          708 c          839 g          417 t
ORIGIN      
        1 agacgctatc cgcaaggcgg gcagcatcgg ccgccccaac ttctacgtcg atgcccgcat
       61 cgttgacaac gccaaccgct ttctggggcc gggcgaggaa ggagagctgg tgctgaaagg
      121 gccgtcgatg tcggcgggct actaccgcaa tcccgatgcc agccaggccg tcatcgacgc
      181 cgtcgccgtt gggatcatcg gcctggagac cgttgcagtc agagttctcg cactcatcgt
      241 acgagcccac gaagcggatg ttgaagccgg cgctgatgcc atccttcgcc cactgggtgg
      301 tgaagttggt cttgatcgcc gggaacacgc cgaagtcgta gttgccctgg gcctggagcg
      361 gcttgtcggg atcgaccagg ttgtcgaact cgaagccgag caggtaggtg ccctcgagag
      421 aggtgcggag gcggccgaac gggaactgac gatcgtaggc caccgagaag tcgatgcccg
      481 aggtgcgggt accgccgacg ttcgccacgg tgtcattgat cgagtcgatc tggaagttca
      541 ccgggttgcg cttgatgcgg tcgcaagcct cttgattgcc acgggtgtag cagtcggcga
      601 ggatcacgcc ggcgccctgc gcctggatgg tgtcggtgat gtcgacgttg aagtaatcga
      661 gggtcatcgc caggcccttg accatcggcg gctcgaacac gaggccggcg gtgaggacgt
      721 tggcggtctc ctcggtcagg ttcgggttac cgccgacctg cgagcgctgc tgcgacaggc
      781 cgaagatcgt gcccaccggg atgccttcgg cgctgcaacg cgcctggacc tcgggcggca
      841 gggtgcgggg ctggtcagcg ccaccgggct gagcatcaca cgggtcatcg acctgcggga
      901 agttgtcggc gttacccgag aacagctcgc cgacgttcgg cgcgcggaac gcagtcgagt
      961 agctgccgcg gatggcgagg ccagccgagg tacggaacag accaccgacc ttgccggtga
     1021 tgccgctgcc gaagttcgag taatcgaacg cgcgggccgc gaggttgagc tcgagccact
     1081 gcacgaggcc ctgaccgacc accggcaccg ccgacagctc ggcgaagcct tcggtcacgt
     1141 tgtagctacc atcggtgggc tcctgcgcgt taccggtagt gtcaccggtc gaggtcagcg
     1201 ggtccggggt gtagccgccg ccctcacgac ggtgatcggc gccgatcgcc agagcgatgt
     1261 cgccacccca cggggtgcgc gccaggcggc cgtgcatcgt gccgagcacg gtctgctgac
     1321 ggttgtagcc ggtcgacacg ccggtgaacg tgacgtagtc gatcatctct tgggtgatgg
     1381 cgttggcatc ggcgccagcg aacacgttca tcggcacgca gccatcgatc ggggcatcgg
     1441 aggtaccaca acgggcgatg ccgtcgctgt cgaagaagct gggtccaagc gcgttgccga
     1501 ggcggctcac gatgaggtta ccctcgttga gctgggtcgc ctgcgtgcgg ccgaagttgt
     1561 aggacagctc ccacttccag tccttgagga cgggcagcga ctcgggcaac gagccatcga
     1621 cgccagccac gatgcggaag gtgtcgatgt tctggagcga gcggcggggg ccgaactcca
     1681 cgaggcggcg gcggtgttct cgccgatcgg gttgtacacg ctgtcggcgc tgatggcgat
     1741 gccagcagag gcggtgaaca gcggctccgg cgccaggcgc tggtccgact tgcggttgat
     1801 gtagctaccc tcgaagaagg tgcgaatctt gtcggtcacc ttgtacgagc cggtgctgaa
     1861 cacgttgtag cgctcggacg gcgtgtacag gtagttctcc ggctggtagt tgtacaggtc
     1921 ggcgggggcg gagaaggcgg cgtagccgtt tccgtcgcgg cggcagacgc ggatgttnnn
     1981 nnnnnnnnnn nnnnnnnnnc agatgttctg acggccgctg tgggtgaacc ggccggcggg
     2041 gatcgccgac gagccgatgt cgacgctcat cttgccctcg tagtcgtact gcttgtcgac
     2101 gttcgagaac tcgcggtcac cggcgaacac cggctcctgg ctctggtaac cagcggcgaa
     2161 gagcacgttg cccttcttgc tggaggtacc agtcaccagg ctcaggtcgt agccgaagcc
     2221 atcaccgcgg gtggtggtgc cggtgtagat ggtggcctcg ctgccattga ggtcctgacg
     2281 ggtgatgatg ttcaccacgc caccgatcgc gtccgagccg tagatggccg aggcgccgtc
     2341 cttgaggacc tcgacgcgct cgatcacggc cagcgggatc
//
LOCUS       DAORNF010000557         2378 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_33610_length_2378_cov_1.761085, whole genome shotgun sequence.
ACCESSION   DAORNF010000557 DAORNF010000000
VERSION     DAORNF010000557.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33610_length_2378_cov_1.761085"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..1092)
                     /locus_tag="PLV68_17820"
                     /pseudo
     CDS             complement(1..1092)
                     /locus_tag="PLV68_17820"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
     gene            complement(1135..>1997)
                     /locus_tag="PLV68_17825"
     CDS             complement(1135..>1997)
                     /locus_tag="PLV68_17825"
                     /inference="COORDINATES: protein motif:HMM:NF015436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HRE03162.1"
                     /translation="AVWLLSKSSDIAVRLMGGDPNMQREEVTEEELRDMVATQVTFTP
                     EQRQIMDGAFQIAKRSLDEVLVPRSDVFVLDSEWTCRDALAALAESGHSRAPVAPRRN
                     LDNVVGVVHLRQLLDLGDELVGSVAVEPPVFPDAAKVLTTLRELQSRRAQMAFVANEH
                     GGIEGVITIEDLIEELVGEIYDETDPDLATVVRGPDGSAILPGRFPIHDLDSLGIALP
                     EGEYATVAGLVLDRLGRIPTEPGDVIDVDGWRITVLAVYRRGISEVRVERIVQAAGTE
                     AGNTAEPDAR"
     assembly_gap    1998..2163
                     /estimated_length=166
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2164..>2378)
                     /locus_tag="PLV68_17830"
     CDS             complement(<2164..>2378)
                     /locus_tag="PLV68_17830"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HRE03163.1"
                     /translation="AFAGTELALVSLREGQLMRLEQRSTTGAILAKLARQPNQFLATI
                     QIGITLAGFLASAAAAVSLSKPLEGKLA"
BASE COUNT          360 a          763 c          740 g          349 t
ORIGIN      
        1 ggcagcggcg gctgcggcgc tgtcctgcca gggacgggtc gcgtagatgg ccgaaccgtt
       61 gacacgcagc caccacccca gagcggtcag ccgggccacc tgggccatcg ggatctggcc
      121 gtcggcggtg ggaccgacgt tgagcagcag gttgccgccg cgggcgacga tgtcgaccag
      181 catccacacc aactcgtcca cgccgagcag gtcgtcgggt gcctccatcc ggttgtagcc
      241 gaacgagcga ccgatgccgc ggcagacctc ccacttgcgc ggtgagatcg cgaccgtcga
      301 gtactccggg gtggagatgt cagcgtggac gttgcccttg agccccatca tgtcgaaccg
      361 atcgttgacc acaccgtcgg gcacgcgggc gtagtagtac tcgaacgtgt cgtgcgcgtc
      421 gagcccggct ggccagccga tgtcgtttca cagcgagcag ggctcgaacc ggtcgatcag
      481 ctcgcggatg tgagcggtgg cataggcccg gtactcggcc gagtcgggga tcgccatcgc
      541 catcgcttcc agaccgtcga tcggcggcgg gacgaaggtc cagtcgatac cgccgctgta
      601 gtacagcccg aaccgcatgc cgcggtcacg caccgcacgg gccaactcgg cgatgtgatc
      661 acgatcggcc atccaccgct cgcggaacgg gttggccgtc gcgctctgcc acatgaggta
      721 tccgtcgtgg tgcttggtga ctggcaccac gtaacgcgcc ccggcgcggg cgaacagctc
      781 ggcccagtga ccgacgttga cgccgttgga gcgctggtgg aactcgtcga cgaagctggc
      841 ataggtccgc tcgccgtaca tctgctcgtg acgcaccgcg gtcgggctgc cctccagcgc
      901 ccacgagttg aggtaccact ccacgtacgg gctgtgacgg aacacctcac gccagcttcc
      961 ggaggcgaac agctcgccca tgtcgaagtc ggtcggcgca tagcacggca ccgcgtacgg
     1021 cccccagtgg atgaagatcc cgaacttggc atcgtcgtac caggccggaa tcggccgccc
     1081 cgtgaagtcc atcgccggag tctgcccgcg tcgcctcgga ccaggcgaac cgggtcagcg
     1141 cgcgtcgggc tcggcggtgt tgcccgcctc ggtgccggca gcctgcacga tgcgctcgac
     1201 acgcacttcg gaaatgcctc gtcggtacac cgccaacacc gtgatccgcc acccgtcgac
     1261 gtcgatcacg tcaccgggtt cggtcgggat gcggccgagg cggtcgagca ccagtccggc
     1321 caccgtcgca tactcgccct cgggtagggc gatgcccagg ctgtcgaggt cgtggatcgg
     1381 aaagcgccca ggcaggatcg cgctgccgtc cggcccgcgc accaccgtgg ccagatcggg
     1441 atcggtctcg tcgtagatct caccgacgag ctcttcgatc aggtcctcga tggtgatcac
     1501 cccttcgatc ccgccgtgct cgttggcgac gaaggccatc tgggcccgac ggctctgcag
     1561 ctcgcgcagg gtcgtcaaca ccttggccgc gtcggggaac accggtggct cgacggcgac
     1621 cgacccgacc agctcgtcgc ccaggtcgag cagttggcgc agatgaacga caccgaccac
     1681 gttgtcgagg ttgcgccgcg gcgcgaccgg tgcacgcgaa tgccccgact cggccaacgc
     1741 cgccagcgcg tctcggcacg tccattcgga gtcgagcacg aacacgtcgc tgcgcggcac
     1801 gagcacctcg tcgagggagc gcttggcgat ctggaacgcg ccgtccatga tctggcgctg
     1861 ctcgggcgtg aacgtgacct gcgtggccac catgtcacgc agctcttctt cggtgacctc
     1921 ttcgcgctgc atgttcgggt cgccgcccat cagccgcacc gcgatgtcgc tggacttcga
     1981 cagcaaccac accgccgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnngccagct tgccttcgag cggtttggac agcgagaccg ccgcggccgc cgaagcgagg
     2221 aagccggcca aggtgatgcc gatctggatc gtggccaaga actgattggg ctggcgagcc
     2281 agcttggcga ggatcgctcc ggtcgtggag cgttgttcga ggcgcatcaa ctggccttcc
     2341 cgcagcgaca ccagcgccag ttcggtgccg gcgaaggc
//
LOCUS       DAORNF010000558         2372 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_33707_length_2372_cov_1.614156, whole genome shotgun sequence.
ACCESSION   DAORNF010000558 DAORNF010000000
VERSION     DAORNF010000558.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33707_length_2372_cov_1.614156"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="PLV68_17835"
     CDS             complement(<1..213)
                     /locus_tag="PLV68_17835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03164.1"
                     /translation="MSDAAAAVVSADQPMPVPSAPPPRRAAAAGATPDVAPAAVVKPA
                     GGRDAAAQSGARQSVVTAVTTNELMPF"
     assembly_gap    240..347
                     /estimated_length=108
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<348..696)
                     /locus_tag="PLV68_17840"
     CDS             complement(<348..696)
                     /locus_tag="PLV68_17840"
                     /inference="COORDINATES: protein motif:HMM:NF023403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03165.1"
                     /translation="MPHHVIIDGSNLATEGRAMPSLKQLNEAIAAYRDDHPDALITVV
                     VDATFGHRIAPSEVPEFEVAIETNSFVAPPAGAVGRGDGFILNIAQKAGAAILSNDSY
                     QEFHGEYPWLFDEG"
     gene            747..1403
                     /locus_tag="PLV68_17845"
     CDS             747..1403
                     /locus_tag="PLV68_17845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HRE03166.1"
                     /translation="MFTAVLWDFGGVILSSPFEAFNHYESQEGLPRDFIRRVNATNPH
                     TNAWALLERNEISPADFDEAFAADSRQLGHEVRGSAVLSLLAGEIRPAMVAALDAVKA
                     AGYKTACLTNNVAGGDGGSSSFASVEAADRQQAIAQVMTRFDAIVESSKVGCRKPEPR
                     FYEIACELLGVTPGECVFLDDLGINLKPAAAMGMTTIKVTDPDAALDDLGAALSLTLR
                     "
     gene            complement(1444..>2372)
                     /gene="kgd"
                     /locus_tag="PLV68_17850"
     CDS             complement(1444..>2372)
                     /gene="kgd"
                     /locus_tag="PLV68_17850"
                     /EC_number="4.1.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441890.1"
                     /note="kgd; produces succinic semialdehyde; part of
                     alternative pathway from alpha-ketoglutarate to succinate;
                     essential for normal growth; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="multifunctional oxoglutarate
                     decarboxylase/oxoglutarate dehydrogenase thiamine
                     pyrophosphate-binding subunit/dihydrolipoyllysine-residue
                     succinyltransferase subunit"
                     /protein_id="HRE03167.1"
                     /translation="RHAALIDYENEHVWVPLADLPGATAKFWIYDSLLSEYAALGYEY
                     GYAHENRDALVLWEAQFGDFINGAQIIIDQYLVAAEDKWDQQNGLVLLLPHGFEGQGP
                     EHSSGRIERFLQSCADDNIQVCNATTAAQYFHLLRRQIHRDGNKPLVLFTPKSALRIP
                     QARSPISDFEQGSFQEVLDDPTPGLDRDAVSRIVFCSGKVAWDAFAERDRRQAPAAIV
                     RIEQLYPLPVDQMRQVISSYPNARELVWLQEEPENMGPWYFVEARTWRIKERGYDLRH
                     VARVESGSPATGSKTIHDQELADLMEETFAGL"
BASE COUNT          368 a          754 c          759 g          383 t
ORIGIN      
        1 gaacggcatc aactcgttgg tggtcacggc ggtgaccacc gactggcgag cgccactctg
       61 tgccgcggca tcgcgcccgc cggcgggctt gaccactgcg gcaggagcaa catcgggagt
      121 tgcaccagcc gccgcggcgc gccgcggcgg aggagcggac ggcaccggca tcggctggtc
      181 ggccgacacc acggccgctg cagcgtctga caccggcttg cgctcgcgac gctggcgggn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngcc cttcgtcgaa
      361 cagccacgga tactcgccgt ggaactcttg gtagctgtcg ttggacagga tcgccgcgcc
      421 cgccttctgg gcgatgttga ggatgaaccc gtcaccgcgg ccaaccgccc cggcaggcgg
      481 ggcgacgaag gagttggtct cgatcgcgac ttcgaactcg gggacctcgg atggcgcgat
      541 gcggtggccg aacgtggcgt cgaccaccac ggtgatcagc gcatcggggt ggtcgtcgcg
      601 gtacgcggcg atcgcctcgt tgagttgctt gaggctgggc atggcccggc cttcggtggc
      661 caggttcgat ccgtcgatga tgacgtggtg cggcataaca catgccagtc aagcatcttc
      721 agcacggtcc ccctacgatc cccccggtgt tcacagcagt cttgtgggac ttcggcgggg
      781 tcatcctgtc cagtccgttc gaagcgttca accattacga gagccaagag ggcctgccgc
      841 gcgacttcat ccgccgagtc aacgccacca atccgcacac caatgcgtgg gcgttgttgg
      901 agcgcaacga gatcagcccg gccgacttcg acgaggcgtt cgcagccgat tctcgccagc
      961 tcggccacga ggtgcgcggc agcgcggtgc tgagcttgct ggccggagag attcgcccgg
     1021 cgatggtcgc ggccctcgac gcggtcaagg ccgcgggcta caagacggcc tgtctcacca
     1081 acaacgtcgc tggtggcgac ggcgggtcga gcagcttcgc ctcggtcgaa gcggccgatc
     1141 gccagcaagc gatcgcgcag gtcatgaccc gcttcgacgc catcgtcgag agcagcaagg
     1201 tgggctgccg caaacccgag ccgcgcttct acgagattgc atgcgaactg ctcggcgtga
     1261 cacccggcga gtgcgtgttc ctcgacgact tgggcatcaa cctcaagccg gctgccgcga
     1321 tgggcatgac cacgatcaag gtcaccgacc cagacgccgc gctcgacgac ctgggtgcgg
     1381 cgctctccct caccctgcgc taactacggg caccctgcac gaatcacagg caccctgcgc
     1441 tgatcacagg cctgcgaacg tctcttccat cagatcggcc agctcctgat cgtggatggt
     1501 cttgctgccg gtggccgggc tgcccgactc gacgcgcgcg acgtggcgca ggtcgtagcc
     1561 acgctccttg atgcgccacg ttcgggcttc gacgaagtac cagggaccca tgttctcggg
     1621 ctcttcctgc agccagacca gttcgcgcgc gttggggtac gacgagatca cctgacgcat
     1681 ctggtcgacc ggcagcgggt acagctgctc gatccgcacg atcgcggccg gtgcctggcg
     1741 gcggtcgcgc tcggcgaacg cgtcccacgc gaccttgccg ctgcagaaga cgatgcggct
     1801 caccgcatcg cggtcgaggc ccggcgtcgg gtcgtcgagc acttcctgga acgacccctg
     1861 ttcgaagtcg gagatcggcg agcgcgcctg ggggatacgc agcgccgact tgggggtgaa
     1921 caacaccagc ggcttgttgc cgtcccggtg gatctgtcgg cgcaacaggt ggaagtactg
     1981 ggccgccgtg gtggcgttgc agacctggat gttgtcgtcg gcacagctct gcaggaaccg
     2041 ctcgatgcgg ccagagctgt gctcgggacc ctgaccttcg aagccgtgcg gcaacagcag
     2101 caccagtccg ttctgctggt cccacttgtc ttccgcagcg accaggtact ggtcgatgat
     2161 gatctgggcg ccattgatga agtcgccgaa ctgcgcctcc cacagcacga gcgcgtcgcg
     2221 gttctcgtgc gcgtagccgt actcgtatcc gagcgcggcg tactcgctga gcaacgagtc
     2281 gtagatccag aacttggccg tggcaccggg cagatcggcc agcggcaccc agacgtgctc
     2341 gttctcgtag tcgatcagcg ccgcgtggcg ct
//
LOCUS       DAORNF010000559         2370 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_33749_length_2370_cov_1.733909, whole genome shotgun sequence.
ACCESSION   DAORNF010000559 DAORNF010000000
VERSION     DAORNF010000559.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33749_length_2370_cov_1.733909"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            92..550
                     /locus_tag="PLV68_17855"
     CDS             92..550
                     /locus_tag="PLV68_17855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03168.1"
                     /translation="MGTAAGECRGVWDGDHVLAERVPIRLDDIPFAVALTDEQGAVHT
                     WRRTLVDPDSGHTVFHVHFGPGQRGEPHWHPSDTLYVFQEGSLEVEGEATYYAGDVRF
                     VRGGFAYGAEVGGPEGCTFIFVSLGPYGRFDPDTDAPPRGRWDAATQTTL"
     assembly_gap    686..697
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<698..1637)
                     /locus_tag="PLV68_17860"
     CDS             complement(<698..1637)
                     /locus_tag="PLV68_17860"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRE03169.1"
                     /translation="MNPLQVISADGHINEPHNLWTDRVQAKFRDRAPRIESFEQGDAW
                     IIEGALDPINFGSNCSAGLPLEQRRAWIRKEEVREGGYLPGPRLADQDADGVDAEILY
                     PTPRVSNSLFWNNADPEFHLDCIRAYNDWLSEFCSYDPDRLWGVAMVPNAGVDDAVAE
                     LDRATSLPGMRGAMLGQYPHGGEMIAPEDDPVWAVAAEKKVPLSIHVSFATQAQGDKK
                     RSKLTGDMRFFDVPSRVSQFVNSGVFDRFPDLRLLLVEVDSGWIPYLREQMNDRFHRG
                     SAADQAKLKRAPGDYFDDNIGSTFITDHYGVANRHAV"
     gene            1746..>1971
                     /locus_tag="PLV68_17865"
     CDS             1746..>1971
                     /locus_tag="PLV68_17865"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRE03170.1"
                     /translation="MSDRQLAQDLITGCNHVNLLVDDLAAAEHFYGTVLGFEALPRPG
                     ISGTGAWFRIGTLQLHLSVVGTPNDERLASN"
     assembly_gap    1972..2030
                     /estimated_length=59
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2167..>2370)
                     /locus_tag="PLV68_17870"
     CDS             complement(2167..>2370)
                     /locus_tag="PLV68_17870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03171.1"
                     /translation="KVAYNQVKALSDRRQLDEWLAVLPRLEEAERAGVSVAHLDNDKV
                     YDLIGLVRVREQLALARRLGIID"
BASE COUNT          377 a          775 c          769 g          378 t
ORIGIN      
        1 ccgagatcgg ctacgacccg gcccgcatcg ccgagttgca cgccaagggc gtcacggtcg
       61 ccgtcggtca cgggctggac gggtgaccgc gatgggcacg gcagcaggcg aatgccgcgg
      121 cgtctgggac ggcgatcacg tgttggcaga gcgggtgccg atccggctcg acgacatccc
      181 cttcgccgtc gccctcaccg acgagcaggg cgcagtgcac acatggcggc gcaccctggt
      241 cgaccccgac tccgggcaca cggtgttcca cgtccacttc ggccctggtc agcgcggcga
      301 gccgcactgg catccgtccg acaccttgta cgtcttccag gagggctcgt tggaggtgga
      361 gggcgaggcc acctattacg ccggcgacgt gcgcttcgtg cgcggcggtt tcgcctacgg
      421 cgccgaggtc ggcggtcccg aggggtgcac cttcatcttc gtcagcctcg ggccctacgg
      481 gcggttcgac cccgacaccg acgctccacc gcgcggccgc tgggacgccg ccactcagac
      541 gacgttgtag agccgcaggg cgttacccgc cagcagcatg taccgctcgt cgtcgggcac
      601 gcccgcgagc tggctgtcga tcgatgcctg ggagctcggc cagtcggagc caccgtgggg
      661 gaagtcgctc gaccacatca tctggnnnnn nnnnnnncga ccgcatgacg attggccacg
      721 ccgtagtggt cggtgatgaa cgtcgaaccg atgttgtcgt cgaagtagtc gccgggagcc
      781 cgcttcagct tggcctgatc ggcggccgaa ccacggtgga accgatcgtt catctgctcg
      841 cgcaggtagg ggatccagcc gctgtcgact tcgaccagca acagacgcag gtcggggaac
      901 cggtcgaaaa cgccggagtt gacgaactgc gagacgcggc tcggcacgtc gaagaaccgc
      961 atgtcgccgg tgagcttcga gcgcttcttg tcgccctggg cctgggtggc gaaggagacg
     1021 tggatcgaca gcggcacctt cttctccgcg gccacggccc acaccggatc gtcctcgggc
     1081 gcgatcatct cgccgccgtg gggatactgg ccgagcatcg caccgcgcat gcccggcaac
     1141 gacgtggccc gatccagctc ggccaccgcg tcgtcgacgc cggcattggg caccatggcg
     1201 acaccccaga gacggtcggg gtcgtacgag caaaactcgg agagccagtc gttgtaggca
     1261 cggatgcagt cgaggtggaa ctccgggtcg gcgttgttcc aaaacaacga gttcgacacg
     1321 cgcggcgtcg ggtacaggat ctcggcgtcg acgccatcag cgtcctggtc ggccagacgc
     1381 ggaccgggca gatacccgcc ctcgcggacc tcttccttgc ggatccaggc ccggcgttgt
     1441 tcaaggggga gcccggccga gcagttgcta ccgaagttga tcggatcgag tgcaccctcg
     1501 atgatccagg catcgccttg ctcgaagctc tcgatgcgcg gggcacgatc gcggaacttg
     1561 gcctgcacgc ggtcggtcca gaggttgtgg ggctcgttga tgtgcccgtc ggcggagatg
     1621 acctgcagcg gattcattgt ctgaatcata tataccttcg ccacccgagg aaagcccgcc
     1681 caccacaatt tccagcgcgc agacgcgctc atcagtacgg ctcaacctct ctatgatcga
     1741 gatgtatgag tgatcgacag ctggcgcagg acctgatcac cggatgcaac cacgtcaacc
     1801 tgctcgtcga cgacctcgcc gctgccgagc acttctacgg cacggtgctc ggcttcgagg
     1861 cactgccccg gccgggcatc tccggtaccg gcgcatggtt ccgcatcggc accctgcaac
     1921 tgcacctcag cgtcgtcggc acgccgaacg acgagcggct ggcctccaac gnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ggtgctgacg
     2041 cgggagccga tgagccgtga gcagttcggg gtcccggtgc gcacggcctt ctgtcgcgac
     2101 ccggccggca acctgatcga gctcaccgac gtcccatcgc tttcgtagcg ccagcggcgg
     2161 gcggccttag tcgatgatgc cgaggcggcg ggcgagcgcg agttgctcgc ggacgcgcac
     2221 caaaccgatc aggtcgtaga ccttgtcgtt gtcgagatgg gccacgctga caccggcccg
     2281 ctcggcctct tccaggcgcg gcagcacggc caaccactcg tcgagctggc ggcggtcgct
     2341 gagcgccttg acctggttgt aggcgacctt
//
LOCUS       DAORNF010000560         2370 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_33751_length_2370_cov_1.447948, whole genome shotgun sequence.
ACCESSION   DAORNF010000560 DAORNF010000000
VERSION     DAORNF010000560.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33751_length_2370_cov_1.447948"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>262)
                     /locus_tag="PLV68_17875"
     CDS             complement(<1..>262)
                     /locus_tag="PLV68_17875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03172.1"
                     /translation="ATLEIAGFTSSGWVSVFIAVYTVAAHTSGRPRAIAVYVFTASMA
                     LLFTAGWFDDKVRISEALSSAVFLLGAYLIGDNLRRRRVATA"
     assembly_gap    263..422
                     /estimated_length=160
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    711..805
                     /estimated_length=95
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <806..>1188
                     /locus_tag="PLV68_17880"
     CDS             <806..>1188
                     /locus_tag="PLV68_17880"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRE03173.1"
                     /translation="FGVPAAHEDDAERAVRAALKMQETLSSFANTAVATAVAPSMQMR
                     VGINTGVALVGTVAGTDYTAMGDVVNTASRLQTLAPPGGVLVGESTYGLTADSIDYEP
                     IGELAAKGRASAVPAWLAKSVVALP"
     assembly_gap    1189..1241
                     /estimated_length=53
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1242..>1522
                     /locus_tag="PLV68_17885"
     CDS             <1242..>1522
                     /locus_tag="PLV68_17885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRE03174.1"
                     /translation="AVSAVNFSVMNSQAVLLAIDGEGGVGKSRLIEELLESITTATPG
                     AFVVRGSAVPYGQANRWWPLASALIDELELEPTTPPDTVREFATLKGRS"
     assembly_gap    1523..1546
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1547..>1900
                     /locus_tag="PLV68_17890"
     CDS             <1547..>1900
                     /locus_tag="PLV68_17890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03175.1"
                     /translation="RSGTTLAELAEALLHMLGYPSRFDDLDPQAARDAVFRATTLVLE
                     QRLSEGPVVMSLTDVHWADPVVLELLEHLLVSLFRAPLVVITTNRPDPDLRWPPLATR
                     AAVVRLPLEPLGPISA"
     assembly_gap    1901..2096
                     /estimated_length=196
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          283 a          627 c          645 g          287 t
ORIGIN      
        1 tcggctgtgg ccaccctgcg gcggcgaagg ttgtcgccga tcagatacgc ccccaacagg
       61 aacaccgcgc tcgacagggc ttcgctgatc cgcaccttgt cgtcgaacca accggcggtg
      121 aacagcaggg ccatcgacgc ggtgaacacg tacaccgcga tcgctcgcgg ccggccgctg
      181 gtgtgcgcag ccacggtgta gacggcgatg aacaccgata cccatccgga actggtgaac
      241 cccgcaatct cgagcgtcgc cannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nngccgcgag gagtacgtcg gccgcggtcg ggtgccgtcg cacccaggtc accggtcgga
      481 aacgaaccac acgcgcacga taacgaagca cctcgcatgt cgtacgatcc cgttcgtgac
      541 acagtgcccg tcgtgccaga gtgcggtccc gccgggtgcc cggttctgct cggcgtgcgg
      601 cacggcgctc ggcaccacca cctccgagga acgacgcgtg ttgacggtgc tgttcgccga
      661 cctggtcggg ttcaccacct tggccgagca tctcgatccg gaacaggtca nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn nnnnngttcg gcgttcccgc cgcgcacgag gacgatgccg
      841 agcgagccgt gcgcgcggcg ttgaagatgc aagagacgct gtcgtcgttc gccaacacgg
      901 ccgtcgccac cgcggtcgcg ccgtcgatgc agatgcgcgt cggcatcaac accggcgtcg
      961 cgttggtggg cacggtcgcg ggcaccgact acacggcgat gggtgacgtg gtcaacaccg
     1021 cgtcgcgcct ccagacactg gcgccgcccg gtggcgtgtt ggtcggcgaa tcgacgtacg
     1081 gcctgaccgc cgactccatc gactacgaac cgatcggcga gctcgcggcc aagggacgtg
     1141 ccagcgcggt gcccgcctgg ctggccaagt cggtcgtcgc gttgcccgnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nggcggtcag cgccgtcaac
     1261 ttctcggtga tgaactcgca ggccgtgttg ttggcgatcg acggcgaagg cggcgtcggc
     1321 aagagccgct tgatcgagga gctgctggag agcatcacca ccgccacccc gggcgcgttc
     1381 gtggtgcgcg gctcggccgt tccgtacggt caggccaacc gctggtggcc gttggccagc
     1441 gcgctgatcg acgagttgga gctcgaaccc accacaccgc ccgacaccgt gcgtgagttc
     1501 gccacgctga agggtcggtc gannnnnnnn nnnnnnnnnn nnnnnncgca gcggcaccac
     1561 cctggccgag ttggccgaag ccctgttgca catgctcggg tacccgtcgc gcttcgacga
     1621 tctcgacccg caggctgctc gcgacgccgt attccgggcg accacgttgg tgttggagca
     1681 gcgcctgtcc gaggggccgg tggtgatgag cctgaccgac gtccactggg ccgatccggt
     1741 cgtgttggag ctgctcgaac atctgctggt cagcctgttc cgtgcgccgt tggtggtcat
     1801 caccaccaac cggcccgacc ccgatctgcg ttggccgccg ctggcgacgc gggcggcggt
     1861 cgtgcgccta ccgctggagc cgctggggcc gatctcggcc nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncgga
     2101 gcagcgggcc gcgatcgaca acgccgcgat cctcgggccg tcgggacaga tcaagtcgct
     2161 ggagtacttc gccacagcgc tcggcaacga cctgacgccc gcggtgctcg acggcttggt
     2221 cgacgcggga ttgctgacga tcagcgggcg gcggtgggac atgcggtgac ggagcatgct
     2281 gctgggggac atgggtcgat cctgtcggcg aggcgaaggg ctcactgcat acggccgggc
     2341 gcggcggacg gatgccggcc gtgccccgtc
//
LOCUS       DAORNF010000561         2360 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_33937_length_2360_cov_1.899349, whole genome shotgun sequence.
ACCESSION   DAORNF010000561 DAORNF010000000
VERSION     DAORNF010000561.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2360
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33937_length_2360_cov_1.899349"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1305)
                     /locus_tag="PLV68_17895"
     CDS             complement(<1..>1305)
                     /locus_tag="PLV68_17895"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HRE03176.1"
                     /translation="AHREGAFKTLRDTPGLQFYEEREFQDSPYPRGPGYWALTRYEDV
                     WAVSRNPQLFCSGKGSNIGDLPQDLNEFFGSMINMDDPKHFRLRSIVSKGFTPKEIAR
                     VNEYVVAKATRIVDEMIERYPGRECDFVETIAAPLPLEIICEMMGIPESDFSQILQWT
                     NVILGAGDPEFGGTLEALMTAALGMFAYAQELGQSRVTNPTDDITSVMMNAEVDGERL
                     TAQEFGSFFILLAVAGNETTRNAISHGMRLLTQHPDQRALWFDDFDANAKTAVEEIVR
                     YATPVIHFRRTATEDTELAGQAIKAGEKVVMWYNSANRDERTFTDPYRFDVLRPLQPQ
                     HVGFGAGGPHFCLGAKSPRYSTRFAVVCRPWRSPASRSTCRASSSTASSAFAAPGSSR
                     RVRRRVSGDHVSSAGRPPGRRWRNGAGTAARSARHRRTGADR"
     assembly_gap    1306..1339
                     /estimated_length=34
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1493..1774)
                     /locus_tag="PLV68_17900"
     CDS             complement(1493..1774)
                     /locus_tag="PLV68_17900"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRE03177.1"
                     /translation="MSNTQTMPGRFHTWTLGDRLRAAREEAGYEVRTFSALTGISPGT
                     ITNYEKDRSRPRAPYLRAWSEVTGFDARWLLTGHIPDGGEPVTKSVTYR"
     gene            1866..2099
                     /locus_tag="PLV68_17905"
     CDS             1866..2099
                     /locus_tag="PLV68_17905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03178.1"
                     /translation="MIDSELEFVTIRDAARILSEARETTRRRVRSGELPSQKLPGDLG
                     PYLILRSDVMRLRDSLIAEAETRLEVLRAGVGS"
     gene            2096..>2360
                     /locus_tag="PLV68_17910"
     CDS             2096..>2360
                     /locus_tag="PLV68_17910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03179.1"
                     /translation="MSAPALDAVSVLVQAIDGFSVRQSVRCLSVAEAVGVAEGWVSPR
                     WSQVSLTAEGMRGWFAVRHLVADGDGVRDSGWRGWGPDGRFLGE"
BASE COUNT          355 a          678 c          844 g          449 t
ORIGIN      
        1 cccgatcggc ccctgttcgg cgatgtcgtg cgctacgagc tgctgtgccg gcgccattac
       61 gccagcggcg acctggggga cgaccggccg aactgacgtg gtcgcctgac acgcgtcgcc
      121 tgacgcggcg tgagctacca ggcgcagcga aggcgcttga tgccgttgat gaagaagctc
      181 tgcaggtact ccggctcgcc ggtgatctcc agggtcggca gacgacggcg aatctcgtcg
      241 aataccgcgg tgatttggcg ccgaggcaga agtgcggccc gccggcgccg aagccgacgt
      301 gttggggctg gagcgggcgg agtacgtcga agcgatacgg atcggtgaag gtgcgctcgt
      361 cgcggttggc cgagttgtac cacatgacca ccttctcgcc ggctttgatc gcctgaccgg
      421 cgagttcggt gtcttcggtc gcggtgcggc gaaagtggat cacgggggtg gcgtagcgaa
      481 cgatctcttc gaccgccgtc ttggcgttgg cgtcgaagtc gtcgaaccac agcgcgcgct
      541 ggtcgggatg ctgggtcagc aggcgcatgc cgtggctgat cgcgttgcgg gtcgtctcgt
      601 tgccggcgac cgcgagcagg atgaagaagc tgccgaactc ttgcgccgtc agacgttcgc
      661 cgtcgacctc ggcgttcatc atcacgctgg tgatgtcgtc ggtggggttg gtgacgcggc
      721 tctggccgag ctcttgggcg taggcgaaca tgcccaacgc ggcggtcatc aaggcttcga
      781 gggtgccgcc gaactcgggg tcgcccgcac cgaggatgac gttggtccac tgcaggatct
      841 ggctgaagtc gctctcggga atgcccatca tctcgcagat gatctccagc ggcagcggcg
      901 cggcgatggt ctcgacgaag tcgcactcac gcccggggta gcgctcgatc atctcgtcga
      961 cgatgcgggt cgccttggcg acgacgtact cgttgacgcg ggcgatctcc ttgggggtga
     1021 agcccttgga cacgatgctg cgcagacgga agtgcttcgg gtcgtccatg ttgatcatcg
     1081 acccgaagaa ctcgttgagg tcctgtggca ggtcaccgat gttgctgccc ttgccactgc
     1141 agaacagctg cgggttacga ctcaccgccc acacgtcctc gtagcgcgtc aacgcccagt
     1201 agccggggcc gcgcgggtac ggggagtctt ggaactcgcg ctcttcgtag aactgcaggc
     1261 ccggggtgtc gcgcaaggtc ttgaacgcac cctcgcggtg ggcgcnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnng atcgagtcga gcgccacggt gggaagttcc atggccgaaa
     1381 tgtagaactg ttgccgcgcc ggcgtcgtat cggagtcgcc acgacggggc ggcgaacagc
     1441 cccgtcaccc aggccggccg acaggcagca cggccgcctg cggagcgatc ggttaccggt
     1501 aggtaaccga tttggtaacc ggctcgcccc cgtcggggat gtgtccggtg aggagccacc
     1561 gagcatcgaa gccagtgacc tccgaccagg cccgaaggta cggggcacga ggccttgacc
     1621 tgtccttctc gtagttggtg atggtgccag gagagatgcc ggtgagcgcc gagaaggtgc
     1681 gcacctcgta cccggcttct tctcgggcgg ctcggaggcg gtcgccgagg gtccaggtgt
     1741 ggaatcgtcc aggcatggtc tgggtattgc tcatgtgcgc aatattacta atcctggtga
     1801 gtctgtcaat atcccgagat attggtactt gacacaaact tgcgacctgt cgtaagtttg
     1861 cacccatgat cgattccgag ttggagttcg tcaccatccg tgacgcagcg agaatcctgt
     1921 cagaagcgcg ggagacgact cgacggcgag tccggtccgg cgaacttccg tcgcagaagc
     1981 tcccgggcga cctcggtccc tacttgattc tccgcagcga cgtcatgcgc ctgcgtgaca
     2041 gcctgattgc tgaggcggag acccgcctgg aggtgctgcg ggcgggggtt gggtcgtgag
     2101 cgcgccggcg ttggatgcgg tctcggtcct cgtgcaggcg atcgacgggt tctcggtccg
     2161 tcagtcggtg cgttgcctgt cggtcgctga ggcggtcggc gtcgcggagg gctgggtgtc
     2221 gccccggtgg tcgcaggtgt cgttgacggc cgagggtatg cgtggctggt tcgctgtgcg
     2281 gcacctggtc gctgacggcg atggtgtgcg ggattcgggt tggcgggggt ggggtccgga
     2341 tggccggttc ttgggtgagg
//
LOCUS       DAORNF010000562         2349 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_34171_length_2349_cov_1.480820, whole genome shotgun sequence.
ACCESSION   DAORNF010000562 DAORNF010000000
VERSION     DAORNF010000562.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34171_length_2349_cov_1.480820"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..620
                     /locus_tag="PLV68_17915"
     CDS             <1..620
                     /locus_tag="PLV68_17915"
                     /inference="COORDINATES: protein motif:HMM:NF015587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HRE03180.1"
                     /translation="NSLAQSVEHPLSGNPFALVVGIGGALWAGMGAMQAAQDAMNRVW
                     DVRHGQMPNFFVKRLRSLALLGSIGLLLIATAVTPQVIGAITSGAIADVLLQLASVAM
                     SVGLFAIAMRVLTEADVSWKEVLPGACIAGVAYTVLQIVGRVYITNTVSRAEDTYGTF
                     AIVIGLLSWMFLIGQVFVIAAEVNVVRARRLWPRGLQTDSLTPNA"
     assembly_gap    695..771
                     /estimated_length=77
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <772..>1053
                     /locus_tag="PLV68_17920"
     CDS             <772..>1053
                     /locus_tag="PLV68_17920"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HRE03181.1"
                     /translation="PGLDGLRALAVTAVVVYHANHEWLSGGFLGVEVFFVISGYLITL
                     LLIGEHERTNRISLREFWKRRARRLLPALFVMMAAVALYMALFYNRPLGR"
     assembly_gap    1054..1079
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1080..>1374
                     /locus_tag="PLV68_17925"
     CDS             <1080..>1374
                     /locus_tag="PLV68_17925"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03182.1"
                     /translation="GLLYVANWFQIVVGQGYTDIEAFAPLRHLWSLAVEEQFYLVWPL
                     VMVVLLRKGRDRLPALAMRLFVTSFAIAVVVALVYVPGDVDVVCAPGQSQGYAT"
     assembly_gap    1375..1409
                     /estimated_length=35
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1410..>1736
                     /locus_tag="PLV68_17930"
     CDS             <1410..>1736
                     /locus_tag="PLV68_17930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03183.1"
                     /translation="LYLSTSSRASGLLLCAAFAMVWRPGALMRGPMRTKGRRLDLIAL
                     AGLAALVVLAMRLHVSSDGGSFGVRFDPWLYRGGFFVTGLATLAIIAAVVHRGARAGR
                     VLGNPLL"
     assembly_gap    1737..1756
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1757..>2118
                     /locus_tag="PLV68_17935"
     CDS             <1757..>2118
                     /locus_tag="PLV68_17935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03184.1"
                     /translation="SYGLYLYHWPVYQIIRKNGFGLSWWKFLLAMAITLPITEASYRF
                     VETPIRQHGWLGWRKLIQRSIRDTVRHRRRVLGALAAGSLAVGFAGMSVAVADNRCVG
                     DVECSIEVGEALQQADTPP"
     assembly_gap    2119..2142
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          291 a          753 c          747 g          376 t
ORIGIN      
        1 gcaacagcct ggcccaatcc gtcgagcatc ccctcagcgg caacccgttc gctctggtgg
       61 tcggcatcgg cggcgcgctg tgggccggga tgggcgccat gcaggcagcg caggacgcga
      121 tgaaccgggt gtgggacgtg cgccacggcc agatgcccaa cttcttcgtc aagcggctgc
      181 gctcgctggc gctgctcgga tcgatcggcc tgctgctcat cgccactgcc gtcaccccgc
      241 aggtgatcgg cgcgatcacg tcaggtgcga tcgccgacgt gttgctgcaa ctggccagcg
      301 tggcgatgtc ggtcgggctg ttcgcgatcg cgatgcgggt gctgaccgaa gctgacgtct
      361 cgtggaaaga ggtgctgccc ggggcgtgca tcgccggggt cgcctacacc gtgctccaga
      421 tcgtcggacg ggtctacatc accaacaccg tcagccgggc cgaagacacc tacggcacgt
      481 tcgcgatcgt catcggcctg ctgtcgtgga tgttcctgat cgggcaggtg ttcgtgatcg
      541 cggccgaggt caacgtggtg agggcgcgac ggctgtggcc acgcggactg caaacggatt
      601 ccctgacgcc caatgcgtga cggttcgccc aacgcctgcc gcaccgcggc gcacagatga
      661 cgccgttcgg gcaccgcggc cgacgatact gatgnnnnnn nnnnnnnnnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngcccggcct
      781 cgacggcctg cgcgcgctgg ccgtcaccgc cgtggtcgtg taccacgcca accacgagtg
      841 gctcagcggc ggcttcctcg gtgtcgaggt gttcttcgtg atcagcggct acctcatcac
      901 gctgctgctg atcggcgagc acgaacgcac caaccggatc agcctgcgcg agttctggaa
      961 acgccgggcc cggcgcctgc tgcccgccct gttcgtcatg atggccgcgg tcgcgctcta
     1021 catggcgctg ttctacaacc gtcccctcgg gcgnnnnnnn nnnnnnnnnn nnnnnnnnng
     1081 cggattgctg tacgtcgcca actggttcca gatcgtggtc ggccaggggt acaccgacat
     1141 cgaggcgttc gcgccgctgc gccatctgtg gtcgctggcg gtcgaagagc agttctacct
     1201 ggtctggccc ctggtgatgg tggtgctgct gcgcaagggc cgcgaccggc tgccggcgct
     1261 ggcgatgcgc ctgttcgtga cctcgttcgc catcgcagtc gtcgtcgcgc tcgtgtacgt
     1321 acccggcgac gtcgacgtgg tgtgcgcgcc gggtcagagc cagggctacg ccacnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnng ctgtacctgt cgacgtccag ccgcgcctcg
     1441 gggctgttgc tctgcgcggc gttcgcgatg gtctggcgtc ctggagcgct gatgcgcggg
     1501 ccaatgcgca ccaagggccg ccgcctcgac ctgatcgcgc tggccgggtt ggctgcgctg
     1561 gtcgtactcg cgatgcggtt gcacgtgtcg agcgacggcg ggtcgttcgg cgtgcgcttc
     1621 gacccgtggc tctaccgcgg cgggttcttc gtcaccggcc tggccaccct ggcgatcatc
     1681 gccgcggtcg tgcaccgcgg tgcacgggcc gggcgggtgt tgggcaaccc gctgctnnnn
     1741 nnnnnnnnnn nnnnnnagct acgggctgta cctgtatcac tggccagtct accagatcat
     1801 ccgcaagaac ggcttcggcc tgtcgtggtg gaagttcctg ctcgccatgg caatcacgct
     1861 gccgatcacc gaggccagct accgcttcgt cgagacgccg atccgccagc acggctggct
     1921 cggctggcgg aagctgatcc aacgctcgat ccgcgacacc gtgcgccacc gtcgccgtgt
     1981 gctcggcgcc ctcgccgcgg ggtcgctggc cgtcggcttc gccggcatga gcgtggccgt
     2041 ggccgacaac cgatgcgtcg gcgatgtcga gtgcagcatc gaagtgggcg aggcgctcca
     2101 gcaggccgac acgccgccnn nnnnnnnnnn nnnnnnnnnn nncggaccga tgtcgtcggt
     2161 cgacgtcccc gtgaccgaac ccgaccccga tgtgaccagc ccgaccgaac cggtggtgcc
     2221 gggcgggccg gtgggcagtg acgttcccac cgattccgtg cccgccgggc cggtcgtcac
     2281 cgatccgcct gcccccgaga cgaccacgcc caacctggcc acgccgacca cggcgacgac
     2341 ggctctggc
//
LOCUS       DAORNF010000563         2348 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_34185_length_2348_cov_1.815962, whole genome shotgun sequence.
ACCESSION   DAORNF010000563 DAORNF010000000
VERSION     DAORNF010000563.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2348
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34185_length_2348_cov_1.815962"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>613)
                     /locus_tag="PLV68_17940"
     CDS             complement(<1..>613)
                     /locus_tag="PLV68_17940"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRE03185.1"
                     /translation="VVIDKPSADDGKIAIGDTITITPTSVAPREFTVVGIVRFASVSS
                     FGGPTWALFDLTTAQEFVLDQPGVVSAIAVVADDGVSQTELQQRIQAQFDPTEVEALT
                     GQQIIEESQSSLARQFSFFTTFLTVFALISIFVGSFVIYNVFKISAAQRMRENALMRA
                     IGARASQVTFSLFVEAFVIGVIGAVLGFVGGVGLAVLITTLLDL"
     assembly_gap    614..716
                     /estimated_length=103
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<717..1056)
                     /locus_tag="PLV68_17945"
     CDS             complement(<717..1056)
                     /locus_tag="PLV68_17945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03186.1"
                     /translation="MFRIALKNTLARKGRLLLSSLAIIAGCAFLSGVLVFGDTINSQI
                     DKLFATAYENVDGYVRSAVSIEGDFGDETRATLPDSDVAIVAAVEGVKSATAQVSGSA
                     VITNAKGDVIG"
     gene            complement(1058..1825)
                     /locus_tag="PLV68_17950"
     CDS             complement(1058..1825)
                     /locus_tag="PLV68_17950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRE03187.1"
                     /translation="MIETTLTAPGPESPGASAAGATQACKFYGSGDSEVRALDGVTVS
                     FDVGKFTAIMGPSGSGKSTLMHTLAGLDSLTSGQVHIGGIDLGGLKDKELTELRRSKV
                     GFIFQAYNLIPTLSAEENIRLPLLLGGSKGDEEWISRVIDTVGLRDRLKHRPSELSGG
                     QQQRVAVARAMASQPTIIFADEPTGNLDSRTGAEILDFMRRAVNELGQTIVMVTHDPV
                     AASYADRVIFLADGKIVDEMHDPTAERVLDRMTRFGE"
     gene            complement(1908..>2348)
                     /locus_tag="PLV68_17955"
     CDS             complement(1908..>2348)
                     /locus_tag="PLV68_17955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRE03188.1"
                     /translation="VLMLTTFDLDDYVYAALRAGVSGFMLKDAPAEQLVDAIRVVAAG
                     DALLAPSVTRRLIEDISTRPSRDATLHAAVLDQLTDRELEVLRLVARGMSNAEIADEL
                     YLGEATIKTHVGRMLTKLGVRDRVQAVVAAYESGVVTPGEQPSG"
BASE COUNT          373 a          719 c          772 g          381 t
ORIGIN      
        1 agatcgagca gcgtggtgat cagcaccgcc aggccgacac cgccgacgaa gccgagtacc
       61 gcaccgatca cgccgatgac gaaggcctcg acgaacagtg agaacgtcac ctgactggcg
      121 cgggcgccga tcgcccgcat caacgcgttc tcgcgcatgc gctgggccgc gctgatcttg
      181 aacacgttgt agatgacgaa gctgccgacg aagatcgaga tcagcgcgaa caccgtcagg
      241 aacgtggtga agaacgagaa ctgacgggcc agcgaggact gcgactcttc gatgatctgc
      301 tgacctgtga gcgcttcgac ctcggtcggg tcgaactggg cttggatgcg ctgttgcagt
      361 tcggtctggc tgacgccatc gtcggccacc accgcgatcg ccgagacgac gccgggctga
      421 tcgagcacga actcttgcgc cgtggtcaga tcgaacagcg cccacgtggg gccgccaaag
      481 ctggacacgc tggcgaagcg gacgatgccg accaccgtga actcgcgggg cgcgacgctg
      541 gtgggggtga tcgtgatcgt gtcgccgatc gcgatcttgc cgtcgtcggc ggacggtttg
      601 tcgatcacca cctnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncacc
      721 gatgacatcg cccttggcgt tggtgatgac ggccgatccg ctgacctgag ccgttgccga
      781 cttcacgccc tcgaccgcgg ccacgatcgc gacgtcggaa tcgggcagcg tcgcccgggt
      841 ctcgtctccg aagtcgcctt cgatcgacac cgccgagcga acgtatccgt cgacgttctc
      901 gtacgcggtc gcgaacagct tgtcgatctg gctgttgatc gtgtcgccga acaccagcac
      961 gccgctgagg aacgcgcacc cggcgatgat ggcgagggag gagagcagga gccggccctt
     1021 gcgggccagc gtgttcttca acgcaatgcg gaacatgtca ctctccgaag cgcgtcatgc
     1081 ggtcgaggac gcgctcggca gtggggtcgt gcatctcgtc gacgatcttg ccgtcggcca
     1141 ggaagatgac ccgatcggcg tagctggctg cgacggggtc gtgggtgacc atcacgatgg
     1201 tctgacccag ctcgttgacg gcgcggcgca tgaagtcgag gatctcggcg cccgtgcggc
     1261 tgtcgagatt gccggtcggc tcgtcggcga agatgatcgt cggctggctg gccatggccc
     1321 gcgcgaccgc aacgcgctgc tgctggccgc cggacagctc gctgggccgg tgcttcagac
     1381 ggtcgcgcag cccgacggtg tcgatcaccc ggctgatcca ttcctcgtca cctttgctgc
     1441 caccgagcag caatgggagc cggatgttct cctcggccga cagcgtgggg atcaggttgt
     1501 aggcctggaa gatgaagccg accttggagc gacggagctc ggtcagctcc ttgtccttga
     1561 gcccgccgag gtcgatgccg ccgatgtgga cctggcccga tgtgagcgag tcgagcccgg
     1621 cgagggtgtg catcagggtg gacttgcccg agcccgacgg ccccatgatg gccgtgaact
     1681 taccgacgtc gaacgacacg gtgacaccat cgagggcgcg cacctcgcta tcgccgctgc
     1741 cgtagaactt gcacgcttgg gtggctccgg cggcggatgc gccgggggac tcggggccgg
     1801 gggcagtgag agtcgtctcg atcatggtga ccgttgtacc ccaggggtgc cacattcgat
     1861 tcctcttcac ggtggatcgg ggtccacctc acgggggaac cgcgatatca accggatggc
     1921 tgctcgccgg gtgtgacgac gccgctctcg taggcggcga ccacggcttg gacacggtcg
     1981 cgcacgccca gcttggtcag catccgtccc acgtgggtct tgatcgtggc ttcgccgagg
     2041 tacagctcgt cggcgatctc ggcgttggac atgccacggg cgaccaggcg gagcacctcg
     2101 agctcgcgat cggtgagctg atcgagcact gccgcgtgca gggtggcgtc gcggctgggg
     2161 cgggtgctga tgtcctcgat caaccgccgt gtcaccgatg gggcgagcag cgcgtccccg
     2221 gcggcgacca cccggatcgc gtcgaccagc tgctcggccg gcgcgtcctt caacatgaac
     2281 ccgctcacgc cggcccgcag cgcggcgtac acgtagtcgt ccaggtcgaa cgtggtcagc
     2341 atcaacac
//
LOCUS       DAORNF010000564         2342 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_34286_length_2342_cov_1.412768, whole genome shotgun sequence.
ACCESSION   DAORNF010000564 DAORNF010000000
VERSION     DAORNF010000564.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34286_length_2342_cov_1.412768"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1333)
                     /locus_tag="PLV68_17960"
     CDS             complement(<1..>1333)
                     /locus_tag="PLV68_17960"
                     /inference="COORDINATES: protein motif:HMM:NF019605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRE03189.1"
                     /translation="VEINLLGPTRVAQTLHSLGVTPHLVAVSTCYVAGNRRGSAPEQL
                     VSDGPFDLGLDWRKEVAASRRLRSDTEAASRTPAQLREFRANAVTELGAAGAPALAAK
                     TEQLRTRWVTDELVEAGRARAASVGWPDAYAFTKALGEQALTETKGDVPVSIVRPSII
                     ESALAEPLPGWIRGFRMAEPVILSYARGLLTQFPGVPEGTIDVIPVDIVVAAIIAVAA
                     TGPSAAAITQVASGGINPLKYGTMVDAIREWFTEHPLYDGEGQPIEVPEWSFPARGKV
                     QQQLQRAKTLVTKAEQTLQKLPLRGTQATWAAKLEERKTDIDRAYEYVQLYGLYTECE
                     AVYQVDNLIGLWGDVGAADRQAFNFDPRSIDWQHYIRNIHLPSIVAHARAKTKPGKNR
                     TDRAERLRRNVLSPDRHVAAFDLENTLIASNVVESFSWLATRRLNTPERIRY"
     assembly_gap    1334..1343
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1344..1721)
                     /locus_tag="PLV68_17965"
     CDS             complement(<1344..1721)
                     /locus_tag="PLV68_17965"
                     /inference="COORDINATES: protein motif:HMM:NF019605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRE03190.1"
                     /translation="MIAERLDGTRIAVTGATGFVGTALVERLLRSVPGCELVLLIRGG
                     KRGATERARRDIFKNDCFERLRRELAVTGEDFDAMIARRVTAINGDVGTDGLGLDDEA
                     RQVLASCHTVIHSAAAVAFDSPLD"
     gene            1832..>2042
                     /locus_tag="PLV68_17970"
     CDS             1832..>2042
                     /locus_tag="PLV68_17970"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HRE03191.1"
                     /translation="MAGRRNTRAAILDAAVDLFGTWGVGATSLDDIAGTVGVAKQTLL
                     YWFASKDELLDAVLEQTAIELATIIE"
     assembly_gap    2043..2100
                     /estimated_length=58
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2101..>2342
                     /locus_tag="PLV68_17975"
     CDS             <2101..>2342
                     /locus_tag="PLV68_17975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03192.1"
                     /translation="RPAVRRPALLGLVREVSRLAPAHAERLTVQVRPFARRAIDYLRD
                     EIAAGRLRQADPSLVVALAYATVTGIATEPEALRAV"
BASE COUNT          325 a          748 c          829 g          372 t
ORIGIN      
        1 cgtagcggat gcgctcgggc gtgttcaacc gccgcgtcgc caaccacgag aagctctcga
       61 ccacgttgct ggcgatcagg gtgttctcca ggtcgaacgc ggcgacgtgg cggtcgggag
      121 acaacacgtt gcggcgcaac cgctcggccc gatcggtgcg gttcttgccc ggcttggtct
      181 tggcgcgggc gtgggcgacg atcgagggca ggtggatgtt gcggatgtag tgctgccagt
      241 cgatcgagcg gggatcgaag ttgaacgcct gtcgatcggc cgcgcccacg tcgccccaca
      301 agccgatcag gttgtcgacc tggtagacgg cttcgcattc ggtgtacagg ccgtagagct
      361 gcacgtactc gtaggcccgg tcgatgtcgg tcttgcgctc ttccagcttg gcggcccagg
      421 tggcctgggt gccgcgtagg ggcagtttct gcagcgtctg ctcggccttg gtgaccagcg
      481 tcttggcccg ctgcaattgc tgctgcacct ttccccgtgc tgggaacgac cactcgggga
      541 cctcgatcgg ctgaccttcg ccgtcgtaca gcgggtgctc ggtgaaccac tcccgaatcg
      601 cgtcgaccat cgtgccgtac ttgagcgggt tgatcccgcc gctggcgacc tgggtgatgg
      661 ccgctgcgct cggcccggtg gcggcgacgg cgatgatcgc ggcgaccacg atgtcgaccg
      721 ggatgacgtc gatggtgccc tcgggaacgc cggggaactg ggtgagcaga ccgcgggcgt
      781 agctgaggat gaccggttcg gccatgcgga agccgcggat ccagccgggc agcggttcgg
      841 cgagggccga ctcgatgatg gacgggcgga cgatgctgac gggcacgtcg cccttggtct
      901 cggtgagcgc ctgctcaccg agggccttgg tgaacgcgta cgcgtcgggc caacccaccg
      961 aggccgcccg tgcccggcct gcttcgacca gttcgtcggt gacccaccgg gtgcgcaact
     1021 gctcggtctt ggcggccaat gccggagcac cggcggcacc gagctcggtg accgcgttgg
     1081 cacggaactc gcgtaactga gcgggggtac ggctggcggc ttcggtgtcg ctgcgcaagc
     1141 gccgcgacgc ggcgacctcc ttgcgccaat cgagaccgag gtcgaacgga ccgtcgctga
     1201 ccagctgctc gggtgcgctg ccgcggcggt tgccggcgac gtagcaggtg ctgaccgcca
     1261 cgaggtgggg ggtgacaccg aggctgtgca gggtctgagc gacacgggtg gggccgagca
     1321 ggttgatctc gacnnnnnnn nnngtcgagg ggcgagtcga aggccaccgc ggcggcggag
     1381 tggatcacgg tgtggcacga cgccagcacc tgcctggcct cgtcgtcgag ccccaacccg
     1441 tcggtgccga catcgccgtt gatcgctgtg acgcggcggg cgatcatcgc gtcgaagtcc
     1501 tcgccggtga cggccagttc gcggcggagg cgttcgaagc agtcgttctt gaagatgtcg
     1561 cgccgggccc gctcggtggc gccgcgcttg ccgccacgga tcagcaacac caactcgcac
     1621 ccgggcaccg agcgcagcag ccgttcgacc agtgcggtgc cgacgaagcc ggtcgcgccg
     1681 gtgacggcga tgcgcgtgcc gtcgagtcgt tcggcgatca cggcgcgcgt ctttctcgtc
     1741 cccagaacat cacggcactg gtctagcaga ttctctacca cttggtagac accgggcgac
     1801 gcgtgcgcca cgggggtgct agacacgggt catggcgggc aggcgcaaca ccagggccgc
     1861 gatcctcgac gcggcggtcg atctgttcgg cacgtggggg gtcggagcga cctcgctcga
     1921 cgacatcgcg ggcaccgtcg gggtggccaa gcagaccctg ctctactggt tcgcgtcgaa
     1981 ggacgaactg ctcgacgccg tgctcgaaca gaccgcgatc gagctggcca cgatcatcga
     2041 agnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 cgtcccgccg ttcgccgtcc ggcgctgctc gggctggtgc gtgaggtcag ccgtctcgcc
     2161 ccggcccacg ccgagcggct caccgtgcag gtgcgtccct tcgctcgccg ggcgatcgac
     2221 tatctgcgcg acgagatcgc cgccggccgc ctacgccagg ccgatccgag cctggtcgtg
     2281 gcgctggcgt acgcgaccgt caccggcatc gcgaccgagc ccgaagcgct gcgcgcggtc
     2341 ga
//
LOCUS       DAORNF010000565         2328 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_34546_length_2328_cov_2.109987, whole genome shotgun sequence.
ACCESSION   DAORNF010000565 DAORNF010000000
VERSION     DAORNF010000565.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2328
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34546_length_2328_cov_2.109987"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1379)
                     /locus_tag="PLV68_17980"
     CDS             complement(<1..1379)
                     /locus_tag="PLV68_17980"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRE03193.1"
                     /translation="MTASPDTAAPRDSGDGQTLRDEFDAIVIGAGHNGLVTATYLARA
                     GMRTLLLEARASVGGTAASEAFGGATVNICNCDHLTFRTTPVIDELGLAGHGLRYLDV
                     EPAQLNMTWDDPHPWPSFHDVDRTIDVLNTLHPAEVEGYRRYLRAAVPAVQMVFAAAA
                     EPPSVRHLVGTALRKKLHGTSALLGWSRKSAADVMRSFFTSEALRAPALAIGPMVWGI
                     SPETPGSGLGALTYAMRHVGTLGRPVGGSGAVTDALAAAFVARGGELRTGHRVSQVRC
                     DSTRVRGVVTADGHEFDAPIVVSACDPHRTFLEWLTDPPAAADRLLRRWRAIPQSQGY
                     EAKIDAVMTAVPRLRVLDDARWASLGIDVSACTTAVAPSLADMHRGALAMSDGAILEH
                     PAMIFNVPTVLDVVINRDTGKSRQDALIEVFMTVDLLNEYTKHAAEWLRAREVPRGLR
                     KQLGCSAHL"
     gene            1414..2223
                     /locus_tag="PLV68_17985"
     CDS             1414..2223
                     /locus_tag="PLV68_17985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HRE03194.1"
                     /translation="MTDQRTPFTRRQRHHRHLGDLSAPELARCLTTRSVIVQPLGAIE
                     QHGPHLPLNTDLVVAQAVAEATIARYGDEVDAWLLPPLALTKSNEHAWSPGTIWLSAE
                     TLMAVLDDIGRCVATTPADRLVFFNGHGGNSALVATANREIRLRHGLKTFLAHPGVPP
                     DQGGASAEGEHGMGVHGGHDETSLMLYLAPELCDMSKAERRIPEAMAANRYVRFGGRV
                     SFGWLSNDFFPDGVIGDPTHADAAWGEQLFEASVTAFGEALREIAAFDYGH"
BASE COUNT          348 a          803 c          823 g          354 t
ORIGIN      
        1 aaaaggtggg cggagcaccc gagctgcttg cggaggccgc gcggcacctc gcgcgcgcgc
       61 agccactcgg cggcgtgttt ggtgtactcg ttgagcaggt cgaccgtcat aaagacttcg
      121 atcagggcat cctggcgcga cttgccggtg tcgcggttga tgaccacgtc gagcacggtc
      181 ggcacgttga agatcatcgc cgggtgctcc aggatcgcgc cgtcgctcat cgccaacgcg
      241 ccgcggtgca tgtcggccag gctgggcgcg accgcggtcg tgcacgccga cacgtcgatg
      301 ccgaggctgg cccagcgtgc gtcgtcgagc actcgcagcc gcggcacggc tgtcatcacc
      361 gcgtcgatct tggcttcgta gccctggctt tgggggatgg cccgccagcg ccgcagcaac
      421 cggtcagcgg cagcgggcgg gtcggtgagc cattcgagga aggtgcggtg cgggtcgcag
      481 gccgacacga cgatgggtgc gtcgaactcg tgaccgtcgg cggtgaccac gccgcgaacc
      541 cgagtgctgt cgcagcgcac ctgtgacacc cggtgtccgg tgcgcaactc gcctccccga
      601 gcgacgaacg ccgctgcgag tgcatcggtg accgcgccgc tgccaccgac aggacgcccg
      661 agggtgccca cgtggcgcat cgcgtaggtg agcgcgccga gccccgaccc gggcgtctca
      721 ggggagatgc cccacaccat cggcccgatc gccagggccg gtgcgcgcag cgcctcactg
      781 gtgaagaagc tgcgcatgac gtcggccgcg ctcttgcggc tccaccccag cagtgcggag
      841 gtgccgtgca gcttcttgcg cagcgcggtg cccaccaggt ggcgcaccga gggaggttcg
      901 gccgcggcgg cgaacaccat ctgcacggcc ggaaccgcgg cgcgcaggta ccggcggtag
      961 ccctcgacct cggccgggtg cagggtattg agcacgtcga tggtgcggtc gacgtcgtga
     1021 aagctgggcc acggatgcgg atcgtcccac gtcatgttga gctgcgcggg ctcgacgtcg
     1081 aggtagcgca gcccgtggcc ggccaggccg agttcgtcga tcacgggggt cgtgcggaac
     1141 gtgaggtgat cgcaattgca gatgttgacg gtggccccgc cgaaggcctc gctggcggcg
     1201 gtaccaccga cgctggcccg cgcttcgagc agcagcgtgc gcatcccggc ccgggccagg
     1261 taggtggcgg tgaccagccc gttgtggccg gccccgatca cgatcgcgtc gaactcgtca
     1321 cgcagagtct gaccatcccc tgagtcccgg ggtgcggcag tgtcggggga tgcggtcact
     1381 ggctcattct tgccgcaccg ctagcgtgtt cgcgtgaccg atcagcgcac cccgttcact
     1441 cgtcgccagc gtcaccaccg ccatctcggc gacctgtcgg cacccgagct cgcgcgctgc
     1501 ctgaccacgc gatcggtcat cgtccaaccg ctgggcgcga tcgaacagca cggtccccat
     1561 ctgccgctca acaccgacct ggtggtggct caagccgtcg ccgaagccac catcgcccgc
     1621 tacggcgacg aggtcgacgc atggttgctg cccccgctgg cgctgaccaa gtcgaacgag
     1681 cacgcctggt cgccgggcac gatctggttg tcggccgaga cgctgatggc ggtgctcgac
     1741 gacatcggcc ggtgcgtggc gaccaccccg gccgatcggc tcgtgttctt caacggccac
     1801 ggcggaaaca gcgcgctggt cgccacggcc aaccgcgaga tccggctgcg ccacggcctc
     1861 aagaccttcc tcgcccatcc cggggtgccg cccgatcaag gcggtgcttc ggccgagggc
     1921 gagcacggca tgggcgtgca cggtggtcac gacgagacca gcctcatgct ctacctggcg
     1981 cccgagctgt gcgacatgag caaggccgag cgccgcattc ccgaagccat ggcagccaac
     2041 cgctacgtcc gcttcggcgg ccgggtcagc ttcggttggc tgtccaacga cttcttcccc
     2101 gacggcgtga tcggcgatcc cacccacgcc gatgcggcct ggggcgagca actgttcgag
     2161 gcgtcggtca cggccttcgg cgaggcgctg cgcgagatcg ccgcgttcga ctatggacac
     2221 tgaacatgga gacactgagt tcggggacgc ggagctgagg gacgccgccg ctgacgcgct
     2281 gcacgtcgat gccgatcggt tgtggcggcg gttgcaggag ctcggcgc
//
LOCUS       DAORNF010000566         2327 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_34559_length_2327_cov_1.405370, whole genome shotgun sequence.
ACCESSION   DAORNF010000566 DAORNF010000000
VERSION     DAORNF010000566.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34559_length_2327_cov_1.405370"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    231..322
                     /estimated_length=92
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <323..776
                     /locus_tag="PLV68_17990"
     CDS             <323..776
                     /locus_tag="PLV68_17990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03195.1"
                     /translation="TVFDWTVQERKDNPFSAFMSNQSDHLYNTIKVSVVAVDLDRPVR
                     LVTLRRGKRPKQSDRPYHVPTGHAALDAAFVCLGFDKLGAAALDDERFASAMLDIEGL
                     RQLRFGDQRLVFTLDRRKPEQLSAAIDAATGLAKLLPTALYQPPASPG"
     assembly_gap    895..905
                     /estimated_length=11
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<906..>1932)
                     /locus_tag="PLV68_17995"
     CDS             complement(<906..>1932)
                     /locus_tag="PLV68_17995"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRE03196.1"
                     /translation="DRYFAAEYNGLSTDEAHEQSVELADEFATECETNNADILQYVGT
                     NNSARDMDTIRRALGEETITYFGFSYGSELGATWATLFPDTVRAAVLDGAADPNADAL
                     ESSLQQSRGFESSLNTFLADCSATPSCAFHNDGDAEGADDALFASLDVTSIPSYDGRP
                     PVERGIATTAVIQAMYGEYLWPQLASALDAAQHGDGTGLTDLYDSYYQRQPDGTYDNS
                     LEAFQVISCADTPERPSVEEEDAESALFAEAAPRLYGDSDGEGGSYFCTFFPESLDPR
                     VAITGAGAGPILVVGTTGDPATPLESSRNMAKALEEGVLLVVRADQHTGYDVNACSMG
                     TVDSYLI"
     assembly_gap    1933..1942
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1943..>2327)
                     /locus_tag="PLV68_18000"
     CDS             complement(<1943..>2327)
                     /locus_tag="PLV68_18000"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03197.1"
                     /translation="TDGPTSSTTDSDRTTAPPTSDGSVPADASILEWEPFGRGLETSS
                     LKVPVDYDDSSKGTFDLFLMRHPARDLSKRIGTLLVNPGGPGFGGSILAQLAPQVYSE
                     DLLDHFDIIGWDPRGTGLSEPFIDCI"
BASE COUNT          327 a          709 c          799 g          379 t
ORIGIN      
        1 caccggacca tcgcccggca tcgcccggcg aatccggctg ccgaggagtg cggagttgcc
       61 ggattccggg caatcgacgc tccgcggacg tgggattcac cgcggtgagg aggggctagc
      121 gttgggggat gttgagttgg ctgcggccgg ggcgcaagcg gcgcgaggcg ctggccgctg
      181 tagcggggtc gatgggaatg tcgttcgacg ccgaacccga ggagccgatc nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nngacggtct tcgactggac ggtgcaggaa cgcaaggaca
      361 acccgttcag cgccttcatg tccaatcagt ccgaccatct gtacaacacg atcaaggtca
      421 gcgtcgtcgc ggtcgatctg gaccgaccgg tgcggttggt gacgctgcgc cgcggcaagc
      481 ggccgaagca gtccgaccgc ccgtatcacg tgccgaccgg tcacgccgcg ctcgacgctg
      541 ccttcgtctg cctcggcttc gacaagttgg gcgcggccgc gctcgacgac gagcgtttcg
      601 ccagcgcgat gctggacatc gaaggcttgc gtcaactgcg cttcggtgat cagcgcctgg
      661 tgttcaccct ggatcgacgc aagcctgagc agctgtcagc ggccatcgac gccgccaccg
      721 ggctggccaa gctgctgccg accgctctct accagccgcc tgcgtcgccc ggttgatctg
      781 gctggcgcgg agtaccgatg tgccggattc ccggcaatcc ccctccgcga gcgctggaat
      841 ggccgcagcg ggggacggtg cgagagttca gcactcggtg ccgggggcgg ggacnnnnnn
      901 nnnnntcgat caggtaactg tcgaccgtgc ccatcgagca cgcgttgacg tcgtagccgg
      961 tgtgctggtc ggccctgacc accagcaaca caccctcctc cagcgccttg gccatgttgc
     1021 ggctgctctc caacggggtg gccgggtcac cggtggtgcc gacgaccagg atcggcccgg
     1081 cgcccgcgcc ggtgatcgca acgcgcgggt cgaggctctc ggggaagaac gtgcagaagt
     1141 agctgccgcc ctcgccgtcg ctgtcgccgt acagccgtgg tgccgcctcg gcgaaaaggg
     1201 ccgactcggc gtcttcctcc tcgaccgatg ggcgctcggg ggtgtcggcg cagctgatca
     1261 cctggaacgc ttcgagcgag ttgtcgtacg tgccatcggg ctggcgctgg tagtacgagt
     1321 cgtacaggtc ggtcaggccg gtgccgtcgc cgtgctgcgc cgcgtcgagc gccgaggcca
     1381 actggggcca caggtactcg ccgtacatcg cctggatgac agccgtggtg gcgatgcccc
     1441 gttcgacggg gggccgcccg tcgtagctgg ggatcgaggt cacgtcgagc gaggcgaaga
     1501 gcgcgtcgtc ggcaccctcg gcatcgccat cgttgtggaa cgcgcacgag ggcgtggcgc
     1561 tgcagtcggc caggaacgtg ttgagcgagc tctcgaagcc ccgcgactgc tgcaggctgc
     1621 tttccagcgc gtcggcgttc ggatcggcgg cgccgtcgag caccgcggcc cgcaccgtgt
     1681 cggggaacaa cgtcgcccac gtggcgccga gctcgctgcc gtagctgaac ccgaagtacg
     1741 tgatcgtctc ttcgcccagc gcgcggcgga tcgtgtccat gtcgcgcgcg gagttgttgg
     1801 tgccgacgta ctggaggatg tccgcgttgt tggtctcgca ctcagtggcg aactcgtcgg
     1861 ccaactcgac cgactgttca tgtgcctcgt cggtgctgag gccgttgtac tcggccgcga
     1921 agtagcggtc gtnnnnnnnn nngatgcagt cgatgaacgg ttcgctgagc ccggtgccgc
     1981 gtgggtccca gccgatgatg tcgaagtgat cgagtagatc ttcgctgtac acctgcggtg
     2041 ccagctgggc caggatcgaa ccgccgaagc cgggaccgcc agggttgacg agcagggtgc
     2101 cgatccgctt ggacaggtct cgcgcggggt gacgcatcag gaacaggtcg aaggtgccct
     2161 tcgacgagtc gtcgtagtcg accggcacct tgaggctcga cgtctccagg ccgcgtccga
     2221 acggctccca ttcgaggatc gacgcgtcag cagggacgga accgtcgctg gtgggcggcg
     2281 cggtggtgcg gtcgctgtcg gtcgtgctcg acgtgggacc gtcggtg
//
LOCUS       DAORNF010000567         2326 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_34578_length_2326_cov_1.755174, whole genome shotgun sequence.
ACCESSION   DAORNF010000567 DAORNF010000000
VERSION     DAORNF010000567.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34578_length_2326_cov_1.755174"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>283
                     /locus_tag="PLV68_18005"
     CDS             <1..>283
                     /locus_tag="PLV68_18005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03198.1"
                     /translation="SAVCYCLGITAVDPSTSATARLDGAADATVDVDRDLRGVAGLTA
                     MVGRHELIAAITARLDQLDAAIATADATLESGEVHGAELEATNAALVRLR"
     assembly_gap    284..522
                     /estimated_length=239
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <523..>1693
                     /locus_tag="PLV68_18010"
     CDS             <523..>1693
                     /locus_tag="PLV68_18010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucosamine-6-phosphate synthase"
                     /protein_id="HRE03199.1"
                     /translation="LFTDGSVRVAGAADRPVFSFVYKAAAEIGELGDNTRRLRASIMA
                     DNLLRLALTAPEARLTLLGHTRWASVGIVSEPNCHPINSDELGNSDELGNSDEIVAST
                     APRPYVIGALNGDVDNHADLKAEHGLSIAGPITTDAKVIPTLVARYGARGNSLVESFR
                     RSVASFEGSVAIGAASADEPEQLLLALRGSGQALYVGLAEDCFVVASEPYGLVEETSR
                     YIRVDGESLQGDATSRGQVFVLSAAQAGELAGVRRLSYDGTELPVTEADVVTAEVTTR
                     DINRGDAPHFLLKEISEAPESFRKTLRGKIVEVVPDGSTSAAVQLRADVGETALPASI
                     ARRLADGSLRKVIVIGQGTAAVAGSSMAAILTPLVAGGLDVDTTVATELSGFHLRL"
     assembly_gap    1694..1742
                     /estimated_length=49
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1797..>2326
                     /locus_tag="PLV68_18015"
     CDS             1797..>2326
                     /locus_tag="PLV68_18015"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate synthase"
                     /protein_id="HRE03200.1"
                     /translation="MLAIVNRRNSDLTDKADGVMYTSDGRDVEMSVASTKAFYSQVAA
                     GALLACAIAEAAGLGSAVRRSELLAGLRDLPGAMRAVLDQRDSIADAARRFAPHRRYW
                     TVVGNGPNTVAAEEVRIKLSELCYKSIACDVTEDKKHIDLSCEPLILVCAAGLVGGTA
                     DDVANEGDAISQQTQDA"
BASE COUNT          323 a          693 c          726 g          296 t
ORIGIN      
        1 acagcgcggt gtgctactgc ctgggcatca ccgcggtcga cccgagcacc agcgcgacag
       61 cacgactcga cggggccgcc gacgcgacgg tcgatgtcga tcgtgacctg cgcggggtgg
      121 ccgggctgac ggcgatggtg gggcgtcacg agttgatcgc cgcgatcacg gcccggctcg
      181 accagctcga tgcggcgatc gccacggccg acgcgacgtt ggagtcgggc gaggtgcacg
      241 gcgccgaact cgaggccacc aatgctgcgc tggttcgctt gcgnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncgctgttc accgacgggt
      541 cggtgcgcgt cgccggcgcg gccgatcgtc ccgtgttcag cttcgtctac aaggctgcgg
      601 ccgagatcgg tgagctgggc gacaacaccc gtcgcctgcg cgcgtcgatc atggccgaca
      661 acctgttgcg cctcgcgctg accgcacccg aggcgcgcct caccctgctc ggtcataccc
      721 gttgggccag cgtcggcatc gtcagcgaac cgaactgcca cccgatcaac agcgatgagc
      781 tcggcaacag cgacgagttg ggcaatagcg acgagatcgt tgcgagcacg gcaccgcggc
      841 cgtatgtgat cggtgcgctc aacggcgacg tcgacaacca cgccgacctc aaggccgagc
      901 acggtctgtc gatcgccggg ccgatcacca ccgacgccaa ggtcattccc acgttggtcg
      961 cccgctacgg agcgcgcggt aactcgctgg tcgagagctt ccggcgcagc gtggccagct
     1021 tcgaaggcag cgtcgcgatc ggcgccgcgt cggcagacga acccgagcaa ctgctgctcg
     1081 cgctgcgtgg gagcggccag gcgctgtacg tcggcttggc cgaggactgc ttcgtggtgg
     1141 ccagcgagcc gtatggcttg gtcgaagaga cgtcgcgcta catccgcgtc gacggcgagt
     1201 cgttgcaggg cgacgcgacc agccgcggcc aggtgttcgt gctgtccgcc gcgcaggcgg
     1261 gagaactcgc tggcgtgcgg cgcctgagct acgacggaac cgagttaccc gtcaccgagg
     1321 ccgatgtcgt gaccgccgag gtcaccaccc gcgacatcaa ccgcggcgac gcgccgcact
     1381 tcctgctgaa ggagatcagc gaggcgccgg agagcttccg caagacgttg cggggcaaga
     1441 tcgtcgaggt cgttccggat ggctcgacct ctgctgcggt gcagctgcgt gccgatgtcg
     1501 gcgagaccgc gctcccggcg tcgatcgccc gccgtctggc cgatgggtcg ctgcgcaagg
     1561 tgatcgtcat cgggcagggc accgccgcgg tcgcgggcag cagcatggcc gcgatcctca
     1621 cgccgctggt ggccggaggg ctcgacgtcg acaccacggt ggccaccgag ttgagcgggt
     1681 tccatctgcg cctnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnaccaccac cgatacgaac cgtacggtcg acctggcccg tggccgcggt gcggcggtgc
     1801 tggccatcgt caaccggcgc aacagcgacc tgaccgacaa ggccgacggg gtgatgtaca
     1861 cctccgacgg ccgcgacgtg gagatgagcg tcgcctcgac caaggccttc tacagccagg
     1921 tggcggcggg ggcgttgttg gcctgcgcga tcgcggaggc agcagggttg ggctctgcgg
     1981 tgcggcggtc cgaactgctc gcgggcctgc gcgatctgcc cggcgcgatg cgcgcggtgc
     2041 tcgaccagcg cgacagcatc gccgacgcgg ctcgccgctt cgccccccat cggcggtact
     2101 ggaccgtggt cggcaacggg cccaacaccg ttgccgccga agaggtgcgg atcaagctca
     2161 gcgagctgtg ctacaagagc atcgcctgcg acgtcaccga ggacaagaag cacatcgacc
     2221 tcagctgtga gccgctgatc ctggtctgcg cggcgggcct ggtcggtggc acggccgacg
     2281 acgtggccaa cgagggagac gcgatctccc agcagaccca ggacgc
//
LOCUS       DAORNF010000568         2322 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_34652_length_2322_cov_1.962947, whole genome shotgun sequence.
ACCESSION   DAORNF010000568 DAORNF010000000
VERSION     DAORNF010000568.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34652_length_2322_cov_1.962947"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>258
                     /locus_tag="PLV68_18020"
     CDS             <1..>258
                     /locus_tag="PLV68_18020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03201.1"
                     /translation="TDVMPVDVMTIAAVLTSPVASAWVAAQAVGSGLTPGTVRVSQRT
                     LGMLPWPAGDLSAAVAALRANDLAACGQAVDRAYGVTADSA"
     assembly_gap    259..356
                     /estimated_length=98
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<357..1289)
                     /gene="map"
                     /locus_tag="PLV68_18025"
     CDS             complement(<357..1289)
                     /gene="map"
                     /locus_tag="PLV68_18025"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HRE03202.1"
                     /translation="MSITEVQTLPQANDPCWCGSGRKYKRCHKPLEGRVLPHEISPYR
                     PVPAHIERPPYADSGHAPRWDEPRVKSPEIIQRMRIAGAAAAEVLRLAGEFVRPGMTT
                     DEIDVYVHDLCIERNAYPSPLNYNGYRKSVCTSVNEVICHGIPDARRLQDGDIVNIDV
                     TTYLGGVHGDTNATFFVGDVDPASRQLVHVTEECMWKGIEAVRPDRPVSDIGKAIEAH
                     AKTHRYGVVRAFIGHGIGEQFHTDVQILHYYDPRASTVMRPGMTFTIEPMITMGTWQH
                     KMWDDGWTAVTADGKRTAQFEHTILVTDDGYDVLT"
     gene            1569..2228
                     /locus_tag="PLV68_18030"
     CDS             1569..2228
                     /locus_tag="PLV68_18030"
                     /inference="COORDINATES: protein motif:HMM:NF020250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAF domain-containing protein"
                     /protein_id="HRE03203.1"
                     /translation="MTARSAGVGATGSRPTATRRRAPQPVVAKRPLRMPEVILGVLLV
                     AGCALGAVLWQRQASTRESFVVAAGPIERGRTLQGADLTVIELGGDTQVFVPAAAAQS
                     LVGLTATADIPAGTPLVAALVAAAPALGADEALTSVALHAGDMPTDLAANDTVRIVVT
                     TNAGAGGVSSTVLLDPIAVVWSVVDARDGVSTVVTLRGSLELSTDIAAAASVRLARVE
                     G"
BASE COUNT          301 a          709 c          814 g          400 t
ORIGIN      
        1 cgaccgacgt gatgccggtc gacgtgatga cgatcgctgc ggtgctgacg tcgccggtcg
       61 cgtcagcgtg ggtggccgcg caggccgtcg gcagcggtct cacaccgggt acggtgcggg
      121 tcagccagcg cacgcttggc atgctgccgt ggccggcagg cgacctgtcc gctgcggttg
      181 ccgccctgcg cgccaacgat ctcgccgcct gcggacaagc cgtcgatcgt gcctacggcg
      241 tcaccgccga ctccgcggnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnggtg
      361 agcacgtcgt agccgtcgtc ggtgaccagg atggtgtgct cgaactgtgc ggtgcgcttg
      421 ccgtcggcgg tgaccgcggt ccagccgtcg tcccacatct tgtgctgcca ggtgcccatc
      481 gtgatcatcg gttcgatcgt gaaggtcatg ccggggcgca tgacggtgga tgcccgcggg
      541 tcgtagtagt gcaggatctg cacgtcggtg tggaactgct cgccgatgcc gtggccgatg
      601 aaggcgcgca cgactccgta gcggtgagtc ttggcgtggg cctcgatcgc cttgccgata
      661 tcgctcaccg gacggtcggg acggacggct tcgatgccct tccacatgca ctcttcggtg
      721 acgtgcacga gttgacggct ggccggatcg acgtcgccca cgaaaaaggt cgcgttggtg
      781 tcgccgtgca cgccgccgag gtaggtggtg acgtcgatgt tgacgatgtc gccgtcttgc
      841 agccggcggg cgtcggggat gccgtggcag atcacctcgt tgacgctggt gcacacgctc
      901 ttgcggtagc cgttgtagtt gagcgggctg gggtaggcgt tgcgctcgat gcacaggtcg
      961 tggacgtaga cgtcgatctc gtcggtggtc atacccgggc ggacgaactc acccgccagg
     1021 cgcaacacct cggctgccgc ggccccggcg atgcgcatcc gctggatgat ctcggggctc
     1081 ttgacccgtg gctcgtccca gcgtggtgca tggccgctgt cggcgtatgg cggccgctcg
     1141 atgtgggccg gcaccgggcg gtacgggctg atctcgtgcg ggaggacgcg gccttcgagg
     1201 ggcttgtggc agcgcttgta cttgcggccg ctgccgcacc agcacggatc gttcgcctgg
     1261 ggcagcgtct ggacctcggt gatcgacatg gctccaggct atgccgtgtg cggcggtcgc
     1321 agttctcatg acggatgacc gcccaatccg ctgcgacgcg tgcgatgcca cggtatttgc
     1381 cacccgcgtg caataaaaac cggggcacat tcacttgaca cagcactcca ctttcgatga
     1441 gatgggtcga ccatcccccg tgtggatttc cgtaggcgga cacgcggaag cactcagggg
     1501 gatttgacaa tcgatcggtc ggtggacggc tgaccccatg gaggcatgaa gtgggagaac
     1561 gtctcgccgt gacggcgcga tctgccggag ttggcgctac cggatcacga ccgaccgcca
     1621 cgcgccgtcg cgccccgcag ccggtggtcg ccaagcggcc gttgcgcatg cccgaggtga
     1681 tcctcggcgt gctcctggtg gccgggtgcg cacttggcgc ggtgctctgg cagcgccagg
     1741 ccagtacccg ggagagcttc gtcgtcgcgg cggggccgat cgagcgcggt cgaacgctgc
     1801 agggtgccga cctcacggtg atcgaactcg gtggtgacac gcaggtgttc gtcccggcgg
     1861 ctgcggcaca gtcgctggtc gggctgaccg caaccgccga cattccggcc ggcacgccgt
     1921 tggttgccgc cctggtcgcg gccgcacccg cgctgggcgc ggacgaagcg ttgaccagcg
     1981 ttgccctgca cgccggcgac atgccgaccg acctcgcggc caacgacacc gtgcggattg
     2041 tggtgacaac gaacgctggg gcgggcggtg tgagctcgac ggtgctgctc gatccgatcg
     2101 ccgtggtgtg gtcggtggtc gacgcccgtg acggcgtgtc gacggtggtc acgctgcgcg
     2161 ggtcgctcga gctcagcacc gacatcgcgg ccgcggcgtc agtgcgtctc gcgcgggtgg
     2221 agggctgacg tggcggtcat cgcagtaggc agcgtgcacg gttctccggg cgctaccact
     2281 ctcgcgctcg acctggcccg caccctcgac ggtgaggtgt tg
//
LOCUS       DAORNF010000569         2322 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_34653_length_2322_cov_1.888399, whole genome shotgun sequence.
ACCESSION   DAORNF010000569 DAORNF010000000
VERSION     DAORNF010000569.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34653_length_2322_cov_1.888399"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(700..954)
                     /locus_tag="PLV68_18035"
     CDS             complement(700..954)
                     /locus_tag="PLV68_18035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03204.1"
                     /translation="MTGSDGRNGGFAGGANANQLAGVAHTSVQIIVKPGTTQGHHRLS
                     GGAALNAPVFSALERTVSSNGRLMFMYDEDRGQHFCGREE"
     gene            complement(1020..>1426)
                     /locus_tag="PLV68_18040"
     CDS             complement(1020..>1426)
                     /locus_tag="PLV68_18040"
                     /inference="COORDINATES: protein motif:HMM:NF013962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HRE03205.1"
                     /translation="QTRFVVVGRDAVPARTGHDRTALVVYQRADEPGSLISILQEFAA
                     RRINLNNLISRPTKQGGLGDYCFIAYADGHIADELVADVLRSLHAKQGAVKFLGSYPV
                     AGDHAAHSAREHADERWRVADDWVATLRARIS"
     assembly_gap    1427..1616
                     /estimated_length=190
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1617..1936)
                     /locus_tag="PLV68_18045"
     CDS             complement(<1617..1936)
                     /locus_tag="PLV68_18045"
                     /inference="COORDINATES: protein motif:HMM:NF012998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase domain-containing
                     protein"
                     /protein_id="HRE03206.1"
                     /translation="MVRIGFLGPLGTFTEQALRSQPDLAAGELVPMRTVPDVLDAVAC
                     GDVDLGFVPIENSIEGTVNFTQDALAFDYDLLIQREVVLDIEHCLLAPPGTTLADVRV
                     VRSI"
     assembly_gap    1959..2092
                     /estimated_length=134
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2093..>2322
                     /locus_tag="PLV68_18050"
     CDS             <2093..>2322
                     /locus_tag="PLV68_18050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-amino-4-imidazole-
                     carboxylate carboxylase"
                     /protein_id="HRE03207.1"
                     /translation="VRSGTISDDEAVGVLKRLPFADLGDSLVDHHRALRQGMPETVYG
                     PGKSADQITRIVAELFAAGTDPVLVTRIDDDQ"
BASE COUNT          361 a          641 c          678 g          318 t
ORIGIN      
        1 gcaacaggcc gagtgcttcg cacatctgcc agtcggagtg tttcagggtg tgcgccgcga
       61 cgaccacatc ttgtggggtg acaggtccga cgaacatatg agaaccatac acacgttcgt
      121 gtcgagatgc aacctcactg acatcacagt tcgagatact gcgacccgga tgtatctctc
      181 ggatcgatgt gtatggcgac gcggacgcgt tcgcccaaag ggctacatgg cattgacgat
      241 gacgaccgtg cggatgtcac cccctgccgc cccgccccga cggcttcaac gacagtcgtg
      301 gatgcgggca tgctgcacaa cggcagcatc gaggccgggt cgtgcggggc aagcggccag
      361 ggggtgcccg gccctgctcc cggcctctgc gccaggagtt gatcgcgtcg gtgcccgcgt
      421 caagaagcaa cgccgaagca gcaacgacgg ccgcatccaa gcgcacgccg gcccgcaccg
      481 agtctgggaa cccccagacc gatagcccac ctcaatcgca gctgcctgct ccgcttcgtt
      541 gcaggaccca cgcgcgcgaa tcgttgacca gcagccaatc gattgggatc agcctgcgtg
      601 gcacgatgtg acctcatcga tacgggccag tcgggtgtgt ctcgtcgagg cctccgtgcg
      661 gaacatcggc agtcatacga gatccgaata tcccgtcagc tattcctcgc gcccgcagaa
      721 gtgctgacca cggtcctcgt catacatgaa catgagtcga ccgttggagg acacggtgcg
      781 ctcaagggcg ctgaagacgg gagcgttcag cgccgcacca cccgataggc ggtgatgacc
      841 ctgcgtcgta cctggcttga cgatgatctg aaccgaggta tgcgccactc cagcgagctg
      901 gtttgcgttg gccccaccgg cgaagccgcc gttgcggcca tcagaaccgg tcacgtcgac
      961 tccgcccgag gcgacgggct gaataccggt gctggcagag tcgaccaggc ctgggacggt
     1021 caggagatgc gggcgcggag ggtggctacc cagtcgtcgg ctactcgcca gcgttcgtcg
     1081 gcgtgctcgc gggcggagtg ggcggcgtgg tcgccggcta ccgggtagga gccgaggaac
     1141 ttgaccgcgc cctgcttggc gtgcagcgag cgcaacacgt cggcaaccaa ctcgtcggcg
     1201 atgtggccgt cggcgtaggc gatgaagcag tagtcgccga gaccaccctg tttggtcggg
     1261 cggctgatga ggttgttgag gttgatccgc cgggcggcga actcttgcag gatgctgatc
     1321 aggctgcccg gctcgtcggc gcgctggtag acgaccaggg cggtgcggtc gtgcccggtg
     1381 cgggccggca cggcatcgcg gccgacgacg acgaaccggg tctggtnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntcga
     1621 tcgagcggac cacccgcacg tcggccagcg tggtaccggg cggagcgagc agacagtgtt
     1681 cgatgtcgag caccacctcg cgctggatca gcaggtcgta atcgaaggcg agcgcatcct
     1741 gggtgaagtt gaccgtgccc tcgatgctgt tctcgatggg gacgaagccg agatcgacat
     1801 cgccgcacgc cacggcgtcg agcacgtcgg gcaccgtgcg catcggcacc aactcgcccg
     1861 cggccagatc gggctggctg cgcaacgctt gctcggtgaa ggtacccaat gggccgagaa
     1921 agccgatgcg aaccacgctg gcctgaaccg gcgatgccnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngtccgatc
     2101 gggcacgatc agcgacgacg aggcggtggg ggtgctgaag cggttgccct tcgccgatct
     2161 gggcgactcg ctggtcgacc accaccgcgc cctgcgccag ggcatgcccg agacggtgta
     2221 cgggccgggc aagagcgccg atcagatcac ccgcatcgtc gccgaactgt tcgccgccgg
     2281 gaccgacccc gtactcgtta cgcgcatcga cgacgaccaa at
//
LOCUS       DAORNF010000570         2318 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_34728_length_2318_cov_1.487848, whole genome shotgun sequence.
ACCESSION   DAORNF010000570 DAORNF010000000
VERSION     DAORNF010000570.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34728_length_2318_cov_1.487848"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="PLV68_18055"
     CDS             complement(<1..212)
                     /locus_tag="PLV68_18055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03208.1"
                     /translation="MDFPTINHTRERLPIDWSFGMMCASFLCESDLERAQDAALRIVQ
                     GCLRDSASDQVVRASAAVLLDRMGNRR"
     gene            complement(293..>582)
                     /locus_tag="PLV68_18060"
     CDS             complement(293..>582)
                     /locus_tag="PLV68_18060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03209.1"
                     /translation="LLVQYFVEDSLEASMREVRGASLIGFSLTDYPDPLTDDGTTLTE
                     TVRIAIDGWIAGIERRVGHHAIADFHIDVFCLPVPSVAAFRDALRSELTRS"
     assembly_gap    583..593
                     /estimated_length=11
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<594..1074)
                     /locus_tag="PLV68_18065"
     CDS             complement(<594..1074)
                     /locus_tag="PLV68_18065"
                     /inference="COORDINATES: protein motif:HMM:NF020452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1837 domain-containing protein"
                     /protein_id="HRE03210.1"
                     /translation="MIDLAGKLAEQAVDYCVPRSRVEDARRHLELTGSADRFVRLSRE
                     ASELFTSLTNSGEGGELLLFLLLERVLGVPQLLCKMSLKTNSEMHVHGTDGVHVKVLD
                     NGRLGLYWCESKLYDDPQSAVRACFDSIAPFLLDEGNGAARRDLLLLRDHLDTGDDVL
                     "
     assembly_gap    1174..1206
                     /estimated_length=33
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1526..1587
                     /estimated_length=62
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1588..1884)
                     /locus_tag="PLV68_18070"
     CDS             complement(<1588..1884)
                     /locus_tag="PLV68_18070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03211.1"
                     /translation="MPSTVDPLPPDRVTDYRDQQFAQRDIADEVAARVDLERFGSAIT
                     GAIAAGVLAEATWMTYSDHRLRVVYLLDRPPVATRYRVAAYRAAKKLQPLIDMYL"
     assembly_gap    1890..2094
                     /estimated_length=205
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2095..2304)
                     /locus_tag="PLV68_18075"
     CDS             complement(<2095..2304)
                     /locus_tag="PLV68_18075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03212.1"
                     /translation="MHPDPYRDLTDAFGHVTRRPPAWTQHPALVGYGDLDGIVAAIRD
                     DHPDPTISDNLIRTLGAIGQEAPDAA"
BASE COUNT          373 a          619 c          662 g          353 t
ORIGIN      
        1 cgcctgttgc ccatccgatc gagtagtacc gctgcggatg cgcggacgac ttgatcgcta
       61 gctgaatcgc gaagacatcc ctggacgatg cgcagcgccg cgtcctgtgc ccgctcaaga
      121 tcggattcac ataggaagct ggcgcacatc atcccgaacg accagtcgat cggtagccgc
      181 tccctggtgt ggttgatcgt ggggaagtcc aactcgactg actgcaggtg gaccgcgcgc
      241 atgagcacgt cgaagcgctc cgacgagagg atccgaaggg ccagctcggg actcacgaac
      301 gcgtgagctc gctgcgaagg gcgtctcgaa acgcggcaac agacggcacc ggaaggcaga
      361 agacatcgat atggaagtcg gcaatcgcgt gatggccgac ccgccgctcg atgccagcga
      421 tccagccgtc aatcgcgatt cttactgttt cagtgagtgt cgtgccgtca tcggtaagcg
      481 ggtcgggata gtcggtgagc gagaatccga tcaagctcgc gccgcgcact tcgcgcatcg
      541 atgcctccaa cgagtcctcg acgaagtact gaaccaatag ctnnnnnnnn nnnccaacac
      601 atcgtcgcca gtatccaagt ggtcacgcag cagcaacaga tcccgtctgg ctgccccgtt
      661 tccctcgtct aggaggaagg gtgcgatgct gtcgaaacac gctcgaaccg cagattgggg
      721 atcgtcatag agcttggact cgcaccagta caggcccagt cggccgttgt cgagaacctt
      781 gacgtgcact ccatccgtgc cgtggacatg catttcggag ttcgtcttca ggctcatctt
      841 gcaaaggagc tgaggaactc cgaggacgcg ttcaagcagg agaaatagca gcagttctcc
      901 gccctctccg ctgttcgtca gggaggtgaa caactccgag gcctcccgag acagccgcac
      961 gaacctgtcg gctgacccgg tcagttccaa gtgccgccga gcgtcctcca ctcgcgagcg
     1021 tgggacgcaa taatccacgg cctgctccgc cagctttcca gcgaggtcaa tcactctggg
     1081 tcgaccgttt ccgtcttttc gcacgaaatg acatcgaaac gtcgctcggg tgccgtcgac
     1141 agggatctct ccgccaactg agaacaggaa gggnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnccac agagttcgcg aaccactggc tggtacccgc gccaacaagc gccgcaacgt
     1261 ccaccaccct tcaaggttcg cgcagccgag ccgacacggc tgggacccgt cgtcgcactg
     1321 acccgcatcc acggcgctcc ccggacggat gccgccctct tgagcgtgta ggcgggagtt
     1381 ccgttccaac cggtgagtgc aggcaaggtc gaaccactcg cgtccccgat agcccccatc
     1441 gccgaactgt ggcgtcgcgc ttgcccaacg cacgaccacg cacggtggtg catgcacggt
     1501 gggcggttgg ccgccagtgg acaacnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnngag atacatatcg atgagtggct ggagcttctt
     1621 ggcggcccgg taggcggcga ctcggtagcg ggtcgctacc ggcgggcggt cgagcaggta
     1681 gacgacgcga agccgatggt cggagtaggt catccacgtc gcctcagcga ggacgccggc
     1741 ggcgatggct cctgtgatgg cgctgccgaa gcgttcgagg tcaacgcgcg cggcgacctc
     1801 gtcggcgatg tcgcgttggg cgaactgctg gtcgcggtag tcggtgacac gatcgggagg
     1861 cagcgggtcg accgtcgacg gcatggctcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncgctgc
     2101 gtcgggcgct tcttgcccga tggcgccgag cgtgcggatc aggttgtccg agattgtcgg
     2161 gtcggggtgg tcgtcgcgga tcgcggcgac gattccgtcg aggtcgccgt atccgacgag
     2221 ggccgggtgt tgggtccagg cgggcgggcg gcgggtgacg tgaccaaagg cgtcggtgag
     2281 gtcgcggtac gggtcggggt gcattgggtg ctcctttc
//
LOCUS       DAORNF010000571         2315 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_34772_length_2315_cov_1.497345, whole genome shotgun sequence.
ACCESSION   DAORNF010000571 DAORNF010000000
VERSION     DAORNF010000571.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34772_length_2315_cov_1.497345"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..625)
                     /locus_tag="PLV68_18080"
     CDS             complement(<1..625)
                     /locus_tag="PLV68_18080"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRE03213.1"
                     /translation="MGLSTVLVDDHTMLRQGLRRGLEAEGIAVLAEAGDGESGVRLAL
                     DHKPDVVIMDVSMPGVDGIEACRRITSADARQCVIILTMHMDRSVIERALRAGAVGYL
                     TKDCSMSEVIEAVRLAANGDTVLSPNLASLMLSEARERMDTEDALISKREEEVLQLVV
                     DGLGTTEIAEHLYISQKTVKNHLASIYDKLNASDRTQAVLAAVRMGIV"
     gene            complement(616..>1045)
                     /locus_tag="PLV68_18085"
     CDS             complement(616..>1045)
                     /locus_tag="PLV68_18085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03214.1"
                     /translation="DLRSDVSETADLAATLREFIDRVRSRSGLDITLTVDQGRRLPLV
                     YERELWNIVREAVINVERHAQATRAWVGWCSTDSTASLSVRDNGVGILFGADRVDAYG
                     LVGMRERAAAIGASLSTESTPDGTVISVDLRIPPGAKTWD"
     assembly_gap    1046..1219
                     /estimated_length=174
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1220..>1437)
                     /locus_tag="PLV68_18090"
     CDS             complement(<1220..>1437)
                     /locus_tag="PLV68_18090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03215.1"
                     /translation="GLAPQARSGLYAVLRARGAVVGLIAVESDADDHFDAKQAEVLHG
                     MSDAIGLSIDNARLFRRLRIASADEERN"
     assembly_gap    1438..1532
                     /estimated_length=95
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1533..1839)
                     /locus_tag="PLV68_18095"
     CDS             complement(<1533..1839)
                     /locus_tag="PLV68_18095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03216.1"
                     /translation="MALIGIVGSPSGLVYRNSLAIVQRQREALDRVGRLYEANGLLFE
                     LQRVAQTLPASLDLDDVLDSTAVRLVDLIDHSMCTVLLVGDAPRRFETARARGYTSRY
                     "
     gene            complement(1830..>2315)
                     /locus_tag="PLV68_18100"
     CDS             complement(1830..>2315)
                     /locus_tag="PLV68_18100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03217.1"
                     /translation="AGVGNEPPAVSWPLVDRRRPPAVDATTATAALPLAPFRIAAFAG
                     SIVRGWSEYAWSNWTLIMTTIALAAYTIAIVARPIVHRNDNRSRLRVIIEAMVVTAFV
                     LVSGGWDSPFALCLLPTAMVAGFVAGNAYSAPGRRSSRCNRSRTAARPTGWVRPRCGW
                     R"
BASE COUNT          330 a          682 c          721 g          313 t
ORIGIN      
        1 ggacgatgcc catccgcacc gctgccaaca cggcctgcgt gcggtcgctg gcgttaagct
       61 tgtcgtagat cgacgccagg tggttcttca ccgtcttctg gctgatgtac aggtgctcgg
      121 cgatctcggt ggtgccgagc ccgtcgacca ccagttgcag gacctcttct tcgcgcttgg
      181 agatcagcgc gtcttcggtg tccatccgct ccctggcctc ggacagcatc agcgaggcga
      241 ggttggggga cagcacggtg tcgccgttgg cggccaggcg aaccgcctcg atcacctcgc
      301 tcatcgagca gtccttggtg agatagccga ccgccccggc gcgcagggca cgctcgatca
      361 ccgagcggtc catgtgcatc gtcaggatga tgacgcactg gcgggcgtcg gcgctggtga
      421 tgcggcgaca ggcttcgatg ccgtcgacac cgggcatcga cacgtccatg atcaccacgt
      481 cgggcttgtg gtcgagggcg aggcgtacac ctgactcgcc gtcgccggct tcggcaagca
      541 cggcgatacc ttccgcttcc aagccgcggc gcagcccttg gcgcagcatc gtgtggtcgt
      601 cgacgagtac cgtgctcagt cccatgtttt tgctcctggg gggatgcgga ggtcgacgga
      661 gatgacggtg ccatcaggtg tcgattcggt ggagagggag gcgccgatcg cggcggctcg
      721 ctcgcgcatg ccgaccaacc cgtaggcatc gacgcggtcg gcgccgaaca ggatgccgac
      781 gccgttgtcg cgcacgctca gcgacgccgt cgagtcggtg ctgcaccagc cgacccaggc
      841 ccgggttgct tgcgcgtgac gctcgacgtt gatcactgcc tcgcgcacga tgttccacag
      901 ctcgcgctcg tagaccaacg gcaggcgacg gccctggtcg acggtcaggg tgatgtcgag
      961 cccgctgcgg ctgcgcaccc ggtcgatgaa ctcgcgtagc gtcgcggcca ggtcggcggt
     1021 ctcgctgacg tcagagcgca ggtcgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnng attgcgctcc tcgtcagccg atgcgatgcg cagacggcgg
     1261 aacaaccggg cgttgtcgat cgacagaccg atcgcgtcgg acatgccgtg cagcacctcg
     1321 gcctgcttgg cgtcgaagtg gtcgtcggcg tcggactcga cggcgatcag ccccaccact
     1381 gcaccgcggg cgcgcagcac ggcgtagaga cccgatcggg cctgcggcgc caacccgnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngatagcga gacgtgtagc cccgggctcg
     1561 cgcggtctcg aaccggcgcg gggcgtcgcc gaccagcagc acggtgcaca tcgagtgatc
     1621 gatcagatcg acgaggcgga cagcggtgga gtcgagcacg tcgtcgaggt cgagcgaggc
     1681 gggcagggtc tgggcgacac gctgcagctc gaacagcagg ccgttggctt cgtacagccg
     1741 gccgacccga tcgagggcct cgcgttggcg ctgcacaatg gcgagcgagt tgcggtacac
     1801 caggccactc ggcgagccga cgatgccgat cagcgccacc cacagcgcgg tcgaacccaa
     1861 cccgtcggcc gggccgcggt cctggatctg ttgcaacgcg atgatcgccg ccccggcgcc
     1921 gagtacgcgt tgccggcgac gaacccggcg accatcgccg tcggcagcag gcacagcgca
     1981 aagggcgagt cccagccgcc agagacgagt acgaacgcgg tgacgaccat ggcctcgatg
     2041 atcacccgca accgagagcg gttgtcgttg cggtgcacga tcgggcgggc cacgatcgcg
     2101 atcgtgtacg cggccagcgc gatcgtggtc atgatcaggg tccagttgga ccaggcatac
     2161 tcggaccacc cgcgcacgat cgagccggcg aacgccgcga tccggaacgg tgcgagcggc
     2221 agtgccgccg tggcggtggt ggcatcgacc gccgggggac ggcgccggtc gaccagcggc
     2281 caagacaccg ccggtggctc gttgccgaca cccgc
//
LOCUS       DAORNF010000572         2305 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_34948_length_2305_cov_1.684889, whole genome shotgun sequence.
ACCESSION   DAORNF010000572 DAORNF010000000
VERSION     DAORNF010000572.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34948_length_2305_cov_1.684889"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>223
                     /locus_tag="PLV68_18105"
     CDS             <1..>223
                     /locus_tag="PLV68_18105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03218.1"
                     /translation="LREWKRRDWGEGGEVFHWWCTESDDAFVGADPVYVTLRDEITEF
                     VGPTVYDLLADHLAAPEPVPVAHPAVRDAR"
     assembly_gap    224..308
                     /estimated_length=85
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<309..>1247)
                     /locus_tag="PLV68_18110"
     CDS             complement(<309..>1247)
                     /locus_tag="PLV68_18110"
                     /inference="COORDINATES: protein motif:HMM:NF012882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HRE03219.1"
                     /translation="VDELSAGDLFIVAGFPEVEIGDTIASVDDPVALPRLVVDEPVLR
                     MTFGVNTSPFAGKEGKYLTSRHLIERLERELLGNVSIKLFQTGSPDVIEVAGRGELQL
                     AVLIESMRREGYELPEVITKEVNGKRFEPQERGVCDVPDEHVGTVTQELAPRKGRIVD
                     LRPGDPGRTIVTFECPSRGLIGFRGLLMTATRGTALLHQHHLGWMPWAGDMPDARGGA
                     MVADRIGATTGYALDKLQLSGQFFVHPGEQVYEGMVVGECAREDEMVVNAVRPKEKNN
                     IRTHSHDDAVKLPAPRIHTLETAIEWIATDELGEATP"
     assembly_gap    1248..1309
                     /estimated_length=62
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1310..2048)
                     /locus_tag="PLV68_18115"
     CDS             complement(<1310..2048)
                     /locus_tag="PLV68_18115"
                     /inference="COORDINATES: protein motif:HMM:NF012239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRE03220.1"
                     /translation="MPKLRNIALVAHVDHGKTTLVDALLRATGTFAGHQAVVERVMDN
                     NDQERERGITILAKAASVHWKGVKINLVDTPGHADFGGEVERALTMVDGVLLLVDAAE
                     GPLPQTRYVLSKALALDLPTIVIINKVDRQDARPEEVLDEVYQLFMDLDASDHHIEFQ
                     VISAVARDARAMVGIGMPAADADLSPLLDTILEQIPEPSGDADTPLQAMVTTLDASEY
                     LGRLAIGRIVNGTMRRGETVALLEEEFG"
BASE COUNT          334 a          707 c          733 g          384 t
ORIGIN      
        1 ccctgcgtga atggaagcgc cgcgactggg gcgaaggcgg cgaggtgttc cactggtggt
       61 gcaccgagtc cgacgacgcg ttcgtcggcg ccgacccggt ctacgtcacg ctccgcgacg
      121 agatcaccga gttcgtcggc cccaccgtgt acgacctgct tgccgaccac ctcgcggcac
      181 cagagccggt gccggtggcc catccggcgg tgcgcgacgc gcgnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnngg cgtggcctcg cccagttcgt cggtggcgat ccactcgatc gcggtctcca
      361 acgtgtggat gcgcggcgct ggcagcttga ccgcgtcgtc gtggctgtgg gtgcggatgt
      421 tgttcttctc cttgggccgc accgcgttga cgaccatctc gtcctcgcgg gcgcactcac
      481 caacgaccat gccttcgtag acctgctcgc cggggtggac gaagaactga ccgctcaact
      541 gcagcttgtc gagtgcgtac ccggtggtgg ccccgattcg gtcggccacc atggccccgc
      601 cgcgagcgtc gggcatgtcc cccgcccacg gcatccagcc caggtgatgc tggtgcaaca
      661 acgccgtgcc gcgggtggct gtcatcagca gaccgcggaa cccgatcaag ccgcggctgg
      721 ggcactcgaa ggtgacgatc gtgcgaccgg ggtctccggg acggagatcg acgatgcggc
      781 ccttgcgcgg cgcgagctct tgggtgacgg tgccgacgtg ttcgtcgggc acgtcgcaca
      841 caccgcgctc ttgcggttcg aagcgcttgc cgttgacctc tttggtgatc acctcgggca
      901 gctcgtaccc ttcacggcgc atcgactcga tcaataccgc gagctgcagc tcgccacgac
      961 cagcgacctc gatgacgtcg ggcgaccccg tctggaacag cttgatcgag acgttgccga
     1021 gcagttcgcg ttcgagccgt tcgatcaggt gacggctggt gaggtacttg ccctccttgc
     1081 cggcgaacgg tgaggtattg acgccgaagg tcattcgcag cacgggctcg tcgacgacca
     1141 agcgcggcag cgctaccgga tcgtcgaccg acgcgatcgt gtcgccgatc tcgacttcgg
     1201 ggaagccagc cacgatgaac aggtcgcccg ccgacagctc gtcgaccnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc gccgaactcc
     1321 tcttcgagca gggccacggt ctcgccgcgg cgcatggtgc cgttgacgat gcggccgatc
     1381 gcgagccgac cgaggtactc gctcgcgtcg agcgtggtga ccatcgcctg cagcggcgtg
     1441 tcggcgtcgc cggacggctc ggggatctgt tcgaggatgg tgtcgagcag cggcgacagg
     1501 tcggcgtcgg cggccggcat gccgatgccg accatcgccc gtgcatcacg ggcaacggcc
     1561 gagatcacct ggaactcgat gtgatggtcg cttgcgtcga ggtccatgaa cagctggtag
     1621 acctcgtcga gcacctcctc ggggcgggcg tcctggcggt cgaccttgtt gatgatgacg
     1681 atcgtcggca gatcgagcgc cagcgccttc gacagcacgt agcgggtctg gggcaacgga
     1741 ccctcggccg cgtcgaccag caacagcacg ccgtcgacca tggtcagcgc tcgctcgacc
     1801 tcgccaccga agtcggcgtg cccgggggtg tcgaccaggt tgatcttgac acccttccag
     1861 tgcaccgacg cagccttggc gaggatggtg atgcctcgct cacgctcttg gtcgttgttg
     1921 tccatcacgc gctcgacgac ggcctgatgg ccagcgaagg tgcccgtggc gcgcaacagg
     1981 gcgtcgacga gggtggtctt gccgtggtcg acgtgagcga ccagggcgat attgcggagt
     2041 tttggcatca gagaaagttc ctgcgcgtcg agcgctacgg gcaccacgag gtcgccaaga
     2101 tcccgggaac catcggcaga gctggatgtg ggggtggggt gccggtcagc ggatgctgat
     2161 cgacggtgtg tcagtcgacc gactcgtcgt cgtcatcgtc gaaggtcacg ccgtacttct
     2221 cggccaacgc cgcgtactca tcgtcctcgt tggaacgacg gtcttcgttt cctccgaatc
     2281 ccagatcaaa tgcgctggga ccagc
//
LOCUS       DAORNF010000573         2300 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_35046_length_2300_cov_2.020045, whole genome shotgun sequence.
ACCESSION   DAORNF010000573 DAORNF010000000
VERSION     DAORNF010000573.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35046_length_2300_cov_2.020045"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1011
                     /locus_tag="PLV68_18120"
     CDS             <1..1011
                     /locus_tag="PLV68_18120"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HRE03221.1"
                     /translation="GMPAVVTTVLAPPRRTGRWWLPLSLVILAAALFLAVHIGPAGTI
                     SSANWNLIWNLRMPRVALAALVGGMLAVAGASYQGAFGNPLADPYLLGVAAGSGLGAT
                     VVFVTSRATTSDWIVDPLPLASFVGGLTAVLLTYLIGAAFGSRRSSLSLLLAGVAITS
                     LATAVQTFVLYRNSDVIREVYSWILGRLNTATWGDVRLVTPYIVISMVVLLLHRRHLD
                     LMRVGDDEAAALGVRVGQVRLIVVMAATFGTAAAVSVSGLIGFVGIVVPHAVRMLAGS
                     SYRRLLPLSIVLGAAFLVLTDIPGRSLSTGAETPIGVVTAFFGAPFFIVVLIRRRLGR
                     TS"
     gene            1008..>1345
                     /locus_tag="PLV68_18125"
     CDS             1008..>1345
                     /locus_tag="PLV68_18125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRE03222.1"
                     /translation="MTATGMAGALQFVDVGVHYGKRQVVHGVNDTVAPGEWLCLIGPN
                     GAGKSSLLRAILGLAVSSGSICVDGTALRDRSPRQRAALVAYVPQVPQLPEEMSAFDY
                     VLLGRNPGPIP"
     assembly_gap    1346..1355
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1356..1829
                     /locus_tag="PLV68_18130"
     CDS             <1356..1829
                     /locus_tag="PLV68_18130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRE03223.1"
                     /translation="GAQPIDAFKAIIDREIAAAVIDRLDMTALAERDLGALSGGEQQR
                     LSIARALTQQAPILLLDEPTSALDIGHQQQALELVDELRHERGLTVISAMHDLTLAGL
                     YADRLTLFHEGHVVRSGVPAEVLVPDVLAEFYGVNVSVVREPDGTVVVIPRRARA"
     gene            complement(1848..>2300)
                     /locus_tag="PLV68_18135"
     CDS             complement(1848..>2300)
                     /locus_tag="PLV68_18135"
                     /inference="COORDINATES: protein motif:HMM:NF013593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03224.1"
                     /translation="LGLVAHGDADEKHLRTPTEASDLIVVALAGRAAEVQEFGEASSG
                     IASDLAFATTVAAQLVGQLGAGGSLISVEAASVPGAGNLVAKVLADSTSRAKVDDILN
                     AAADRAACMVLEYREALMALSRGLLERDELSGNDVHAIVTATAHTSAA"
BASE COUNT          287 a          797 c          811 g          395 t
ORIGIN      
        1 ggtatgccag cggtggtcac caccgtcctg gccccgccgc gccgcaccgg gcgctggtgg
       61 ctgccgctct cgctggtgat cctggccgcg gcgctgttcc tcgcggtgca catcggcccg
      121 gccggaacga tctcgtcggc caactggaac ctgatctgga acctgcgcat gccccgcgtg
      181 gcgttggcgg ccctggtcgg cggcatgctc gccgtcgccg gtgccagcta ccaaggggcc
      241 ttcggtaacc cgctcgccga cccgtacctg ttgggggtgg ctgccggttc gggccttggg
      301 gccacggtgg tgttcgtcac cagccgggcc accacgtcgg actggatcgt cgacccgctg
      361 ccgttggcgt cgttcgtcgg cggactgaca gccgtgctgt tgacgtacct gatcggcgcc
      421 gcgttcggca gccggcggtc gtcgctgtcg ttgctgctgg ccggtgtggc gatcacctcg
      481 ttggccaccg cggtgcagac gttcgtgctg taccgcaaca gcgatgtgat ccgcgaggtc
      541 tacagctgga tcctgggccg cctcaacacg gccacgtggg gcgacgtgcg cctggtgacc
      601 ccgtacatcg tgatcagcat ggtggtgttg ctgctgcatc gccgccatct cgatctcatg
      661 cgcgtcggcg acgacgaggc cgccgcgctc ggggtgcggg tcggccaggt gcggttgatc
      721 gtggtgatgg cggccacgtt cggcacggct gccgcggtgt cggtcagcgg tctgatcggc
      781 ttcgtcggca tcgtcgtgcc ccacgccgtg cggatgctgg ccgggtcgag ctaccggcgg
      841 ctgctgccgc tgtcgatcgt gctcggcgcg gcgttcttgg tgctgaccga cattcccggc
      901 cgcagcctca gcaccggggc ggagacaccg atcggggtcg tcaccgcgtt cttcggcgcg
      961 ccgttcttca tcgtcgtgct catccgccgc cgcctgggcc gcacgtcatg actgcaacgg
     1021 gtatggccgg cgcgctgcaa tttgtcgacg tcggcgtcca ctacggcaag cgccaggtgg
     1081 tgcacggggt caacgacacc gtggcacctg gcgagtggct gtgcctgatc ggcccgaacg
     1141 gcgccggcaa atcgtcgctg ctgcgggcga tcctcggcct ggccgtgtcc agcgggtcga
     1201 tctgcgtcga cggcacggcg ttgcgcgacc gctcgccgcg ccagcgtgcg gcgctggtgg
     1261 cgtacgtgcc ccaggtaccg cagctccccg aggagatgtc ggccttcgac tacgtgctgc
     1321 tcggtcgcaa ccccggcccg ataccnnnnn nnnnnggcgc ccagccgatc gacgccttca
     1381 aggccatcat cgaccgcgag atcgcggcgg cggtgatcga ccggctcgac atgaccgcct
     1441 tggccgagcg cgatctcggc gcgctgagcg gtggcgaaca gcagcggttg tcgatcgctc
     1501 gcgccctcac ccagcaggcg ccgatcctgc tgctcgacga accgaccagc gcgctcgaca
     1561 tcggccatca gcaacaggcc ttggaactgg tcgacgagct gcgccacgag cgcggcctca
     1621 ccgtgatctc ggcgatgcac gacctgacgt tggccggctt gtatgccgac cgcttgacgc
     1681 tgttccacga aggccacgtc gttcgttcgg gcgtcccggc cgaggtgctg gtgcccgatg
     1741 tgttggccga gttctacggc gtcaacgtca gcgtggtccg tgaacccgac ggcacggtgg
     1801 tcgtcatccc gagacgcgct cgcgcctgac gccgaccgag agccgggtca ggcggcagac
     1861 gtgtgcgcgg tcgcggtgac gatggcgtgg acgtcgttgc cgctcaactc gtcgcgctcg
     1921 agcagaccgc gcgacagggc catcagcgcc tcgcggtact ccagcaccat gcacgcggcc
     1981 cggtcggcgg cggcgttgag gatgtcgtcg accttggccc ggctggtcga gtcggccagc
     2041 accttggcca cgaggttgcc cgcgccgggc accgacgcgg cctcgacgct gatcaggctg
     2101 ccacccgcgc caagttggcc cacgagttgg gccgcaacgg tggtggcgaa cgcaaggtcg
     2161 gacgcgatgc cgctgctggc ctcaccgaac tcctgtactt cggccgcccg cccggcgagc
     2221 gcgaccacga tcaggtcgct ggcctcggtg ggggtgcgga ggtgcttctc gtcggcgtcg
     2281 ccgtgagcaa caagccccag
//
LOCUS       DAORNF010000574         2298 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_35098_length_2298_cov_1.690147, whole genome shotgun sequence.
ACCESSION   DAORNF010000574 DAORNF010000000
VERSION     DAORNF010000574.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2298
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35098_length_2298_cov_1.690147"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>217)
                     /locus_tag="PLV68_18140"
     CDS             complement(<1..>217)
                     /locus_tag="PLV68_18140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03225.1"
                     /translation="HTGFGDRVTYQQVALGIDKTADQSAPLDDLLLLTQAGETFLWSL
                     TPTVSSSVPGTLAINVRVVDTLPATAMF"
     assembly_gap    218..409
                     /estimated_length=192
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<410..2229)
                     /locus_tag="PLV68_18145"
     CDS             complement(<410..2229)
                     /locus_tag="PLV68_18145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03226.1"
                     /translation="MPDPDGLCPTAAAGNDCSVADDRVTSNDLTTFFYAVSASDLQGS
                     PVQPVGEVILEVVAHPAGGAVLAWEIDSIKGVPAICLTAGEGAARPSSISTDPDGSIR
                     LVCNLGNFNTADLHTAPISLRASGASPNGSSFTIDARAYSADNNAQLPAELAGGPIEI
                     SAAPRFNLGKRGNTTLGDPPGRAVQYSNLVNVVNPVTGLAEPTYTVGYGVAIEATGDG
                     RGAEALTDQFGFLELIDPSYAPWGARPRGCIARNTVAEMRSNGQTTLPLPGGPAVPDA
                     PYAVADSGTWSCLSGGDQTSGLWPISITAADTSGSHYPSQDVSGNALTHKLVAVQVIE
                     VYYPISALMKAHVPGWNDGDPVVVGNYPVTNCLGGFDPVSVSGVSNYGAGFEPGWDGT
                     SASGDNCRTHDIIIRAPGGFWKGYGQVPDSGDPLTVDVSVGYPCLRPELIPTGAAQCW
                     TGDGFTAAHEELYGWLVMGDDGSPLAMAKSCVTCGTTRCRRSLRTPRAQGLARWFSSL
                     FATGSTLRTSLCSMPSATATRSNMGAWRVRPAMVGRSATTCSADRSTSTPGASPASGT
                     RSRPSPPTAATRPRLPEPQSSPPIPRLRDGRWTTSSSSDWR"
BASE COUNT          350 a          709 c          707 g          340 t
ORIGIN      
        1 gaacatcgcc gtcgcgggca gcgtgtcgac cacccgaacg ttgatcgcaa gcgtgccggg
       61 gaccgacgac gacaccgtcg gagtcagtga ccacaagaac gtctcacccg cttgcgtgag
      121 cagcaacagg tcgtccagcg gcgcgctctg gtcggcggtc ttgtcgatgc cgagtgccac
      181 ctgctgatag gtcacacggt cgccgaagcc ggtgtgcnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc gccaatcgga
      421 cgatgacgat gtcgtccagc gaccatcccg cagccgcggg atcggtggtg aactctgcgg
      481 ttccggcagt ctgggccgcg tggccgcagt cggcggcgaa ggtcttgatc gcgtcccact
      541 cgccggggat gcgcccggtg tcgaggtcga tcggtccgcc gagcacgtcg ttgccgaacg
      601 acccaccatg gctggccgca cccgccaggc gcccatattc gaccgtgtag ccgttgccga
      661 cggcatcgaa cacagggacg ttcggagagt tgatcctgtc gcgaagagag aactgaacca
      721 gcgtgccagc ccctgggccc gcggtgtacg gagagatcgt ctgcaccgag ttgtcccaca
      781 cgtcacacag ctcttcgcca tcgccagcgg agaaccatcg tcgcccatga ccagccaccc
      841 gtacagttct tcgtgcgccg ccgtgaaccc atccccggtc cagcattgtg cggcaccggt
      901 cgggatcagt tcgggccgaa ggcaggggta cccgacgctc acatcgacgg tcagtgggtc
      961 gccgctgtcg ggcacctgcc cgtaaccctt ccaaaagccg ccgggggcgc ggatgatgat
     1021 gtcgtgggtg cggcagttgt cgccagacgc gctcgtgccg tcccaacccg gctcgaaccc
     1081 ggcgccgtag ttgctcacac ccgagaccga cacggggtcg aagcccccga ggcagttcgt
     1141 gaccgggtag ttgccgacca cgaccgggtc gccgtcgttc caccccggca cgtgagcctt
     1201 catcagtgcc gagatcgggt agtagacctc gatgacctgg acggccacca gtttgtgggt
     1261 cagagcgttg cccgacacgt cctggctggg gtagtgcgac ccactggtat cggccgcggt
     1321 gatcgagatc ggccacagtc cgctcgtctg atcgccgccc gacaggcagc tccaggtccc
     1381 actgtccgcg accgcatagg gggcatcggg aaccgctggc ccgcctggga gcggcaacgt
     1441 cgtctgcccg ttggaccgca tctccgccac ggtgttgcgg gcaatgcaac cccggggcct
     1501 cgcgccccac ggggcatagc tggggtcgat caactccagg aacccgaact gatcggtcaa
     1561 cgcctctgca ccgcgaccgt ccccggtcgc ctcgatcgcg accccgtagc cgacggtgta
     1621 cgtcggttcg gccagaccag tcacggggtt gacgacgttc acgaggttcg agtactgcac
     1681 agcccgaccg ggcgggtcgc cgagggttgt gttgccgcgc ttgcccaggt tgaaccgcgg
     1741 ggccgcggag atctcgatcg gaccaccggc gagctcggcg gggagctgcg cgttgttgtc
     1801 ggccgaatag gcgcgggcgt cgatcgtgaa gctcgatccg ttgggcgacg ctccgctggc
     1861 ccgcaacgag atgggggccg tgtgcagatc ggccgtgttg aagttgccga ggttgcacac
     1921 caggcggatc gaaccgtcag ggtcggtgga gatcgacgac ggtctggccg cgccttcgcc
     1981 cgccgtcaag cagatcgcgg gcacaccctt gatcgagtcg atctcccacg ccagcacggc
     2041 ccctccagcc gggtgcgcca ccacttccag gatcacctcg cccaccggct gcaccggcga
     2101 accttgcagg tcggaggccg acaccgcgta gaagaacgtc gtcaggtcgt tgctggtgac
     2161 acgatcgtcg gcaaccgagc agtcgttgcc cgccgcagca gtggggcaga ggccgtcggg
     2221 gtcgggcaac gggacagttg atgggcctga gctcgtcatc tcctcgtcaa gttggagcga
     2281 gacatcgatc acgtggag
//
LOCUS       DAORNF010000575         2295 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_35152_length_2295_cov_1.698661, whole genome shotgun sequence.
ACCESSION   DAORNF010000575 DAORNF010000000
VERSION     DAORNF010000575.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35152_length_2295_cov_1.698661"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>254)
                     /locus_tag="PLV68_18150"
     CDS             complement(<1..>254)
                     /locus_tag="PLV68_18150"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRE03227.1"
                     /translation="VLRLVTERGDTVVVNPPVYPPFYAFITHDGRRVADAPLGGDGRI
                     DFDVLDATFAQARAGGGNVAYLLCNPHNPTGAVHTRAELE"
     assembly_gap    255..295
                     /estimated_length=41
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<296..607)
                     /locus_tag="PLV68_18155"
     CDS             complement(<296..607)
                     /locus_tag="PLV68_18155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03228.1"
                     /translation="MVTRPTALTGTTPTGVGNPLAELSLAELRGRTSLKWRAHPDDVL
                     PLWVAEMDVPLAPPIADALRSAIDRGDTGYPAGHGYARAVAAFAAERWGWTGVRVEQT
                     AL"
     gene            746..1537
                     /locus_tag="PLV68_18160"
     CDS             746..1537
                     /locus_tag="PLV68_18160"
                     /inference="COORDINATES: protein motif:HMM:NF025587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate and iron-dependent oxygenase
                     domain-containing protein"
                     /protein_id="HRE03229.1"
                     /translation="MAFQEIPVVSFADWTTPAGDRAQFADRLRSICHDTGFFRLIDHG
                     VDQGFLADYFAALRAFFALSEDDKARIDKTNSPWFRGWERVGAELTDNSVDYREQIDV
                     WTDLDPRPRDVSPSYLRLEGPNQWPGDTVVPGFRALVERFQSEMEALADELLAAMAVG
                     LGLEPDHFEQLFGERRMALVKLIHYPPTPAGGAGVNAHHDTGFLTLLWQHGVGGLQVQ
                     NQDGDWIDVPADGDAIVVNLGEMLQSMTGNYFVATTHRVITEAER"
     gene            1565..1807
                     /locus_tag="PLV68_18165"
     CDS             1565..1807
                     /locus_tag="PLV68_18165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03230.1"
                     /translation="MTTRLDPLPLGPAYTEAVARSVHHRDAGFMARRDELLAGNRGTT
                     SAPADTYGQQLWNYFTRSYPDLVRRHHPDLAATTID"
     assembly_gap    1928..1989
                     /estimated_length=62
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          353 a          704 c          756 g          379 t
ORIGIN      
        1 cctccaactc ggcgcgggtg tgcacggcgc cggtcgggtt gtgcggattg cacagcagat
       61 aggcgacgtt gccgccaccc gcgcgggcct gggcgaaggt tgcgtcgagt acgtcgaagt
      121 cgatccgccc gtcaccgccc agcggggcat cggccacccg ccgaccgtcg tgagtgatga
      181 atgcgtagaa cggtggatac acgggtggat tgacgaccac ggtgtcacct cgctcggtca
      241 ccaggcgcaa gaccnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncagcg
      301 cggtctgctc gactcggacg ccggtccacc cccaccgctc ggcggcgaag gcggcaaccg
      361 cccgggcgta gccgtgcccg gccgggtagc cggtatcgcc gcgatcgatc gcgctgcgca
      421 atgcgtccgc gatcggtgga gcgagcggca cgtccatctc ggcaacccac aagggcaaca
      481 cgtcgtctgg atgggcccgc cacttcaggc tcgtacgccc tcgcaactca gccaacgaca
      541 actcggccaa cggattcccc acaccggtcg gagtggtgcc cgtcagagct gtgggccgcg
      601 tcaccatgcc ccggacgtta gacctccgtg agcaggccgc ccgccgtcga catcccaagg
      661 tgtgttcggt tgacctcgct ggtgggggaa ctgagcgcgg tcgggcgagc acgtggggga
      721 gcggggacag gggtaccgtg gcggggtggc gtttcaggag attcccgtcg tctcattcgc
      781 ggactggacg actcccgctg gtgaccgtgc ccagttcgcc gatcggttgc ggtcgatctg
      841 tcacgacacc ggcttcttcc gcctgatcga ccacggcgtc gatcagggtt tcctggccga
      901 ctacttcgcc gcgttgcgcg ccttcttcgc gttgagcgaa gacgacaagg cgcgtatcga
      961 caaaaccaac tcgccgtggt tccgtgggtg ggagcgcgtc ggtgcagaac tgaccgacaa
     1021 cagcgtcgac taccgcgagc agatcgacgt gtggaccgat ctcgaccctc gtccgcgtga
     1081 cgtctcgccg agctatttgc gtctggaagg acccaatcag tggcctggcg acaccgtggt
     1141 gccaggcttt cgtgcgctgg tcgagcggtt ccagagcgag atggaggcgt tggccgatga
     1201 actgctggcc gccatggcgg tgggactcgg cttggagccc gaccacttcg agcagctgtt
     1261 cggcgagcgg cggatggcgc tggtcaagct gatccactat ccgccaacgc cggctggagg
     1321 agccggtgtc aacgcccacc acgacaccgg gttcctcacg ttgttgtggc agcacggtgt
     1381 cggtggtctg caggtgcaga accaggacgg tgactggatc gacgtgcccg ccgacggcga
     1441 cgcgatcgtg gtcaacctcg gtgaaatgtt gcagtcgatg accggcaact acttcgtcgc
     1501 cactacccat cgtgtgatca ccgaagccga gcgctagtcg tcggcctact tccacggccc
     1561 gcagttgacg acgagactcg acccgttacc gctcggcccg gcgtacaccg aggcggtagc
     1621 gcgcagcgtt caccaccgtg acgcagggtt catggcccgc cgtgacgagc tcctcgccgg
     1681 caatcgcggc accacttcgg ccccggccga cacctacggc cagcagctgt ggaactactt
     1741 cacccgcagc taccccgacc tggtccgccg tcaccacccc gacctcgccg caacgacgat
     1801 cgattgaccc ctgtgacgcg ggtcaactga acgcacatcg tcggccagga gcccactgtg
     1861 ttcgactgac ccctgctacg ggggtcagtc gaacatgcct ggccgcggct gctggagctg
     1921 tgtcgggnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnt gggttgaccc gccgcgggcg actgaacagg cctggccgcg gttggtggac
     2041 gtcggtcggg gttggccccg gttatcggag ccgactgaag ccggcttggt gcgggggccg
     2101 cgtcgggcgg cgggggatgg gacttccggc gcgatgaggg aagtaaggat aggaactgtt
     2161 cttgatacac tcacccgagt gatgaaggtg tcagggaaga cctcgatgag gccgaaccgt
     2221 tcgttttggt gggtgtgcag tgctggtgcg gtggctgcgc tttctgggct cgtgttgggg
     2281 gggtgtggca gcgac
//
LOCUS       DAORNF010000576         2291 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_35242_length_2291_cov_1.497317, whole genome shotgun sequence.
ACCESSION   DAORNF010000576 DAORNF010000000
VERSION     DAORNF010000576.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35242_length_2291_cov_1.497317"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..986)
                     /locus_tag="PLV68_18170"
     CDS             complement(<1..986)
                     /locus_tag="PLV68_18170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03231.1"
                     /translation="MTRKLTLSVIGSAVVAVGLLVLCLAVGWKPALGLDLRGGVSVTL
                     RPVPGQDYTADSLKLTVQRIEERVNSLGVGEPEIIQQDDAIVVNLPGVNDQNQALELA
                     KVNGRVNLRPVLACQTVFPTPETPTESTTSTESTVATDASTSTTVATSETDAQSSGAD
                     APVITSPRGSIGIGGARHVASTPSTDETTLTTDGTGATVESTVAADVPTPAADETTTT
                     AAIVVDPAVTTTTQVFSDPNTTQVLYTRTAKDGTRELCQVGPSQGDGTVFSEDSAEAQ
                     IIQGGWGVAVDLKSGSSGEGIWNALASQCYSGQSSCPTRRLAIELDGVIQSAP"
     gene            complement(1006..>1389)
                     /gene="yajC"
                     /locus_tag="PLV68_18175"
     CDS             complement(1006..>1389)
                     /gene="yajC"
                     /locus_tag="PLV68_18175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HRE03232.1"
                     /translation="MLASTGEKSSSGGSIISLLFLPLMFVGIYFLMIRPQRRRMKQQS
                     AMQSALAEGDEVITTSGMYGFITGFEGDIVWLEIDDNIQIRVARGAVQRKVDTSAAAA
                     PAVTESPKSTPASSTEIDELTDDLK"
     assembly_gap    1390..1450
                     /estimated_length=61
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1525..>1905)
                     /locus_tag="PLV68_18180"
     CDS             complement(1525..>1905)
                     /locus_tag="PLV68_18180"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HRE03233.1"
                     /translation="RYLMGVGDPASLVEAVGLGVDQFDCVMQTRLGRHGTALTSTGKV
                     HVKNAKYALDDGPIDPACVCEVCQRHSRGYVRHLFQVGEPTGARLVSLHNIAWTLQLM
                     ERMRAAIAAGTFDALRREVLAVWG"
     assembly_gap    1906..2081
                     /estimated_length=176
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2082..>2291)
                     /locus_tag="PLV68_18185"
     CDS             complement(<2082..>2291)
                     /locus_tag="PLV68_18185"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-guanine transglycosylase"
                     /protein_id="HRE03234.1"
                     /translation="DGVTFKSTYDGSTHRFTPESAVATQELLGADIQMVLDVCAPLPS
                     PPSVIRLALDRTTAWAARAKAAHRRD"
BASE COUNT          300 a          669 c          747 g          338 t
ORIGIN      
        1 ggggccgact ggatcacacc gtcgagctcg atcgccagac ggcgggtggg gcagctggac
       61 tggcccgagt agcactggct ggcgagcgcg ttccagatgc cctcgccgga cgaaccgctc
      121 ttgaggtcga ccgcgacgcc ccagccgccc tggatgatct gggcctcggc cgagtcctcg
      181 gagaacacgg tgccgtcgcc ctggctgggg ccgacctggc acagctcgcg ggtgccgtcc
      241 ttggcagtgc gggtgtacag cacctgggtc gtgttgggat cggagaacac ctgcgtcgtc
      301 gtggtgacgg ccgggtcgac cacgatggcg gcagtcgtcg tcgtctcgtc cgcggcaggc
      361 gtgggcacgt cggcggcgac cgtcgactcg acggtggcgc cggtgccatc cgtggtgagt
      421 gtggtctcgt cggtgctcgg cgtgctggcg acgtgacgag ccccaccgat gccgatcgag
      481 ccgcgcggtg acgtgatgac cggcgcgtcc gcacccgacg attgggcatc ggtttcagag
      541 gtggcaacgg tggtggacgt actcgcgtcg gtggcgacgg tcgactccgt cgacgtcgtg
      601 gattcggtcg gcgtctccgg tgtcgggaac acggtctggc aggccagtac cgggcgcagg
      661 ttgacccgtc cgttgacctt ggccagttcc agcgcctggt tctggtcgtt gacgcccggc
      721 aggttgacga cgatcgcgtc gtcctgctgg atgatctcgg gctcgccgac gccgagcgag
      781 ttgactcgct cttcgatgcg ctggacggtc agcttgaggc tgtcggcggt gtagtcctgc
      841 cccggcaccg ggcgcagcgt gaccgacaca cccccgcgca ggtcgaggcc gagcgccggc
      901 ttccagccga cggccaggca cagcacgagc agaccgacgg cgacgacggc gctgccgatg
      961 accgacagcg tgagtttgcg agtcatgacg gtgtgcgccc cgcgatcact tgagatcgtc
     1021 ggtcagctcg tcgatctcgg tcgacgacgc tggcgtggac ttgggtgact ccgtgacagc
     1081 gggtgctgcc gctgcggacg tgtcgacctt gcgctgcacc gcgccgcggg cgacgcggat
     1141 ctggatgttg tcgtcgatct cgagccacac gatgtcgccc tcgaagccgg tgatgaagcc
     1201 gtacatcccc gaggtggtga tgacctcgtc gccttcggcc agcgcggact gcatcgcgct
     1261 ctgttgcttc atgcggcgcc gctgggggcg gatcatcaag aagtagatgc cgacgaacat
     1321 cagcgggagg aacagcaggc tgatgatgct tccgccagaa gacgacttct cgccggtgga
     1381 ggcaagcatn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn ccgacgagcc tagtgcccat ggcccccgat tcgcggctca tcggtcgcca
     1501 cgggccgctg atcgggccca acggtcaacc ccagacggcc agcacctcgc ggcgcagggc
     1561 gtcgaaggtt cccgcggcga tcgcggcccg catccgctcc atcagctgca aggtccaggc
     1621 gatgttgtgc aggctgacca ggcgcgcgcc ggtcggctcg cccacctgga acaggtgccg
     1681 cacgtacccg cgactgtgac gctggcacac ctcgcagaca caggccggat cgatcgggcc
     1741 gtcatccagc gcgtacttgg cgttcttgac gtgcaccttg ccggtggacg tcagcgcggt
     1801 gccgtgacgg ccgagacggg tctgcatcac acagtcgaac tggtcgacgc cgagtccgac
     1861 cgcttcgacc agcgaggccg ggtcgccgac acccatcagg tagcgnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngtcgcggcg gtgggccgcc
     2101 ttggcccgcg ccgcccatgc ggtggtgcga tcgagggcga ggcggatgac ggacggcggg
     2161 gacggcagcg gagcgcacac gtcgagcacc atctggatgt cggcaccgag cagctcctgg
     2221 gtggcgaccg cgctctccgg ggtgaagcgg tgcgtggagc cgtcgtaggt gctcttgaac
     2281 gtgacgccgt c
//
LOCUS       DAORNF010000577         2288 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_35309_length_2288_cov_1.560233, whole genome shotgun sequence.
ACCESSION   DAORNF010000577 DAORNF010000000
VERSION     DAORNF010000577.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2288
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35309_length_2288_cov_1.560233"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="PLV68_18190"
     CDS             complement(<1..387)
                     /locus_tag="PLV68_18190"
                     /inference="COORDINATES: protein motif:HMM:NF016328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HRE03235.1"
                     /translation="MSSPTTTGRPRLVHNKDEVLATAGSRYTYCANPLALRDAKAEGL
                     SRLALVGMGCQTSSPPIMWDRKAGKVSKPFQFNIGLLCSKTFDDSIFPELFEAKYGLK
                     KSEMVKMNIKGVFQIWMRDGSYHEIDL"
     gene            complement(413..847)
                     /locus_tag="PLV68_18195"
                     /pseudo
     CDS             complement(413..847)
                     /locus_tag="PLV68_18195"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
     assembly_gap    1047..1214
                     /estimated_length=168
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1215..>1599)
                     /locus_tag="PLV68_18200"
     CDS             complement(<1215..>1599)
                     /locus_tag="PLV68_18200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HRE03236.1"
                     /translation="IRDVVQNHLLQVLTLCAMEPPVAPDSSHLQDEKAKVLAAMRPVD
                     PHQVVRGQYVGYRDENGAAKDSTIETFAAMRLEIDSWRWAGVPWYIRAGKGLAAAATE
                     VIVEFKEPPAMLFDEAGGPPPTRNLV"
     assembly_gap    1600..1609
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1610..2283)
                     /locus_tag="PLV68_18205"
     CDS             complement(<1610..2283)
                     /locus_tag="PLV68_18205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HRE03237.1"
                     /translation="MSNQPAADALVLFGATDDLSKKKLMPALYHMQRHGRLNVPVIGV
                     ARSTWTDRDFRRHAEESVLAAVPDADPAVIQAVCKRLDLVQGDYSDPHTWATLAKKLD
                     HHHSRVAVFYMAIPPDMFPEVATALASVGLNDRGRIVVEKPFGRDLASAIELNQTLHN
                     VFPEDRIFRIDHYLGKESVEDLLVFRFSNTLLEPVWNRNYVRSVQITMSETIGVEGRG
                     SFYDGVG"
BASE COUNT          315 a          675 c          714 g          406 t
ORIGIN      
        1 gagatcgatc tcgtggtagc tgccgtcgcg catccagatc tggaacacgc ccttgatgtt
       61 catcttgacc atctcggact tcttcagccc gtacttggct tcgaacagtt ccgggaagat
      121 cgagtcgtcg aaggtcttgc tgcacagcag cccgatgttg aactggaacg gcttgctgac
      181 cttgccggcc ttgcgatccc acatgatggg cggtgacgac gtctggcagc ccatacccac
      241 caacgccagg cggctgagtc cctcggcctt ggcgtcgcgt aacgcgagtg ggttggcgca
      301 gtaggtgtag cggctgccgg ccgtggcgag cacctcgtcc ttgttgtgca ccaggcgtgg
      361 ccttccagtg gtcgtcgggc tcgacaccgc tgaccatcgc gccttcgatg tagtcgtgct
      421 cgaacagcca gaccagcatc gccgacacga atccgccgtc ctggccgatc ttgtgcatcg
      481 tgtcgtcgct ggcgcgggtg aggtacagct cgcgccagat gcccgccatc tcgtcgggct
      541 cgcgcacccg cccgtgcagg tgcatgtcgg ccgaggtctc ccacgcccga aagcgcgggc
      601 aggcgcgggt gcaggtggtg caccccttct cgccgtggat gcagttgtcg gggccgagct
      661 cttcttccag gtggaacggc ttgtacttgc cctcttcgtg gttgtacccg atgacgtcgt
      721 ggggacaggt gactacgcag cccgcacatc ccgtacagag tccggtggtg accacttctg
      781 cgtacagctc tttccactgt gcgctccagc ggccgggctt ggtgggtgct gcctcggtca
      841 gggtcatgcg atgaatctac gcccctcgcg ccgcgcccag ggaaacccgt tggttcacgg
      901 agagacgggc agccacgcgt caccgttgag aatgcggtcg gcctcgccgg gaccccacgt
      961 gccggtgaag tacggatgga tgggggcggg gtggtcgaga gcgggttgca cgatgtgcca
     1021 ggtgcgctcg acgacgtctt ggcgggnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnngcacca ggttgcgcgt gggtggtggc ccgccggctt cgtcgaacag
     1261 catcgccgga ggctccttga actcgacgat cacctcggtg gctgctgcgg caagaccctt
     1321 gccggcccgg atgtaccacg gcacgcccgc ccagcgccac gagtcgatct ccagccgcat
     1381 cgccgcgaag gtctcgatcg tggagtcctt ggctgcaccg ttctcgtcgc ggtacccgac
     1441 gtactggccg cgcaccacct ggtgcgggtc gaccgggcgc atcgccgcca gcaccttggc
     1501 cttctcgtct tgcaggtgtg agctgtcggg cgcgacgggt ggctccatcg cgcacagagt
     1561 cagcacctgc aggaggtggt tctgcaccac gtcgcggatn nnnnnnnnnc ccacaccgtc
     1621 gtagaagctg ccgcggccct cgacgccgat cgtctcgctc atggtgatct gcacgctgcg
     1681 cacgtagttg cggttccaca ccggttcgag cagcgtgttc gagaaccgga acaccagcag
     1741 atcctcgacg ctctccttgc cgagataatg gtcgatacgg aagatgcgat cttcggggaa
     1801 cacgttgtgc agggtctggt tgagctcgat cgccgaggcg aggtcgcggc cgaagggctt
     1861 ctcgaccacg atccggccgc ggtcgttgag tcccactgat gcgagcgcgg tggcgacctc
     1921 ggggaacatg tcgggcggga tcgccatgta gaacaccgcg acgcgcgagt ggtggtggtc
     1981 gagcttcttg gccagcgtgg cccacgtgtg cgggtcgctg tagtcgccct ggaccagatc
     2041 gagtcgcttg cacacggcct ggatcaccgc cgggtcggca tcgggtactg ccgcgagcac
     2101 gctctcttcg gcgtggcggc ggaagtcgcg gtcggtccac gtgctgcggg ccacaccgat
     2161 caccggcacg ttgaggcgac catgccgttg catgtggtac agcgcgggca tcagcttctt
     2221 cttgctgagg tcgtctgtgg cgccgaacag gacgagcgcg tcggcagcag gttggttgct
     2281 catcggcc
//
LOCUS       DAORNF010000578         2281 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_35449_length_2281_cov_1.682839, whole genome shotgun sequence.
ACCESSION   DAORNF010000578 DAORNF010000000
VERSION     DAORNF010000578.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35449_length_2281_cov_1.682839"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>364
                     /locus_tag="PLV68_18210"
     CDS             <1..>364
                     /locus_tag="PLV68_18210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRE03238.1"
                     /translation="RRDGDDWVINGQKIWTSFGASADYCYLICRTSTEGPPHAGISEI
                     IVPMDTHGIEVRPITDMTTNRHFCEVFFTDVRVPIANLVGTEGAAFKQTMRQLEHERG
                     GIDRLVSNHQLYRDALERA"
     assembly_gap    365..416
                     /estimated_length=52
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <417..674
                     /locus_tag="PLV68_18215"
     CDS             <417..674
                     /locus_tag="PLV68_18215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRE03239.1"
                     /translation="RIGRILVIREVLKQAPAGFSAATKCFCTEHEQRVAEFVARTLGP
                     EATVWNHAVHGLLYTPGYTIMGGTSNIMRNILGERVLGLPR"
     gene            complement(865..938)
                     /locus_tag="PLV68_18220"
     tRNA            complement(865..938)
                     /locus_tag="PLV68_18220"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(902..904),aa:Val,seq:gac)
     assembly_gap    1027..1089
                     /estimated_length=63
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1090..2226)
                     /locus_tag="PLV68_18225"
     CDS             complement(<1090..2226)
                     /locus_tag="PLV68_18225"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRE03240.1"
                     /translation="MSDGAGRTGMFSSLRIRNFRLFFGGQLISQIGNWLTMIAQTLLV
                     LELTDSGIALGLLAACQFGPVLVMSAWTGLIADRSDKRRLLIIVQTGAMVQSFALAIV
                     ASADAPKVWMVYVLAIGGGCLTAFDSPTRRAYVVEMVPSAYVQNAVSLNSALMTGARV
                     VGPAVAGLLIILVGYSWTFAIDGISYIAVIAGLLMMRPSENRAPLVTPKGKGQIREGL
                     RYVRTVPELWVPLVMIAFVGTFAFNFQTVIPLLVTRSLHSSDRTYTVLYSVLSVGSLL
                     GALLAARRIGVTVRMITIAGACFGVAMLAMAVSPSVPIVMPAAIAVGLFSTTFMTSST
                     ALMQLKASPAMRGRVLALQVMVTMGSTPVGAPILGWVSEHFGARA"
BASE COUNT          412 a          762 c          686 g          306 t
ORIGIN      
        1 cccgccgcga cggcgacgac tgggtgatca acggacagaa gatttggacc agcttcggcg
       61 cgtcggccga ctactgctac ctgatctgcc gcaccagcac cgaaggccca ccgcacgccg
      121 gcatcagcga gatcatcgtc ccgatggaca cccacggcat cgaggtcagg ccgatcaccg
      181 acatgaccac caaccgtcac ttctgcgagg tgttcttcac cgacgtgcgg gtgcccatcg
      241 ccaacctggt cggcaccgag ggcgcggcgt tcaagcagac gatgcgccaa ctggaacacg
      301 agcgcggcgg catcgaccgg ctggtgtcca accaccagct ctaccgcgac gcgctggagc
      361 gggcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncgca
      421 tcgggcgcat cctggtcatc cgcgaggtgc tcaagcaggc accggccggg ttctcggctg
      481 cgaccaagtg cttctgcacc gagcacgaac agcgcgtcgc cgagttcgtc gcccgcaccc
      541 tcggccccga ggccaccgtc tggaaccacg ccgtccacgg cctgctgtac acgcccggtt
      601 acacgatcat gggcggcacc agcaacatca tgcgcaacat cctcggcgaa cgagtcctcg
      661 gcctcccccg ctgacaggct gcgctcgaac aactcgtcga cgaagtgtcg gcgatggtgt
      721 tggcgatggt gtcggccatg atgtcggcga ggatcaacgc cggcgagccg ctggacacga
      781 tcatccacac cgccaccgcc ccgacgcgca acgtcccgga cgcggcgatg ccctccgctg
      841 gggagggcat cgtggcttgc ggggtgggca cgacggggct cgaacccggg acctccaccg
      901 tgtcatggtg gcgctctaac caactgagct acgcgcccgc aacgggacga cagagtatca
      961 gtgcacggtg cggaccggct accgcgcctc ggcgaatctt ggctcggacg acaccgacgg
     1021 cagcggnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1081 nnnnnnnnng gcgcgggcgc cgaagtgctc gctgacccaa cccaggatgg gagctcccac
     1141 cggtgtgcta cccatcgtca ccatcacctg taaggccaac acccggccgc gcatcgcggg
     1201 cgacgccttc aactgcatca acgccgtgga cgacgtcatg aacgtggtgc tgaacagacc
     1261 gaccgcgatc gcggccggca tcacgatggg gaccgacggc gataccgcca tcgccaacat
     1321 cgcgacgccg aagcacgcgc cggcgatggt gatcattcgc accgtgacgc cgatgcggcg
     1381 ggccgccagc agtgccccga gcagcgagcc gacgctgagc accgagtaga gcaccgtgta
     1441 ggtgcggtcg ctgctgtgca agctgcgcgt gaccaggagc gggatcacgg tctggaagtt
     1501 gaacgcgaac gtgccgacga acgcgatcat caccagcggt acccacagct cgggcaccgt
     1561 gcgcacgtaa cgcagcccct cacggatctg gcccttgccc ttcggggtca ccaacggcgc
     1621 ccggttctcg ctggggcgca tcatcaacag accggcgatg accgcgatgt agctgatgcc
     1681 gtcgatggcg aaggtccacg aatagccgac gaggatgatc aacaaacccg cgaccgccgg
     1741 gccgacgact cgtgcaccgg tcatcagtgc actgttgagg ctgacggcgt tttgcacgta
     1801 ggccgacggc accatctcga ccacgtaggc acgccgcgtg gggctgtcga acgccgtcag
     1861 gcatccgccg ccgatcgcca acacgtacac catccacacc ttgggcgcgt ccgccgacgc
     1921 cacgatcgcg agtgcgaacg actgcaccat cgcgcctgtc tgcacgatga tcagcaggcg
     1981 tcgcttgtcg gagcggtcgg cgatcaaccc ggtccatgcc gacatcacca acaccgggcc
     2041 gaactggcac gcggcgagca agccgagcgc gatgccgctg tcggtcaact cgagtaccag
     2101 cagagtctgg gcgatcatcg tcaaccagtt gccgatctgg gagatcaact ggccgccgaa
     2161 gaagagccgg aaattgcgga tgcgcagcga gctgaacatg cctgtgcggc ccgctccatc
     2221 gctcacgccg tcaggctgtg gtgtctccgc ggcagcggat gcgctcaccg cgatcctccg
     2281 c
//
LOCUS       DAORNF010000579         2281 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_35453_length_2281_cov_1.404762, whole genome shotgun sequence.
ACCESSION   DAORNF010000579 DAORNF010000000
VERSION     DAORNF010000579.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35453_length_2281_cov_1.404762"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="PLV68_18230"
     CDS             <1..384
                     /locus_tag="PLV68_18230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03241.1"
                     /translation="RRARHARNPHPLRAGGVTMNTTAITGTVTTSPWHRIRAFVPTTT
                     FDVRVDFARRAYTFRIRALNDLARTAQTLQVGDHVAITGYLHLEPASTDPTALHFEIV
                     AYEIDHQPGRPAAGGDQSQTSENEP"
     gene            381..695
                     /locus_tag="PLV68_18235"
     CDS             381..695
                     /locus_tag="PLV68_18235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03242.1"
                     /translation="MIADPATVVLTAHVAHWADHHPARTVWIIDCLHRHNNGDWGDLD
                     PDDTNTNRHALRHHDGRILSRYPIPTELADPTIDDDAIWIITDDLADPDTLTTILWPS
                     DY"
     gene            740..>1060
                     /locus_tag="PLV68_18240"
     CDS             740..>1060
                     /locus_tag="PLV68_18240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03243.1"
                     /translation="MGDDLRWHEELRELYRPRHLRVLLIGESPPASDNRARRFFYAPE
                     LTGHDSLFRSAALAAYGYTKADLAERSKTSVLLRLQADGIWLIDAVEHAINHHAANAR
                     TAAIR"
     assembly_gap    1061..1094
                     /estimated_length=34
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1318..>1991)
                     /locus_tag="PLV68_18245"
     CDS             complement(1318..>1991)
                     /locus_tag="PLV68_18245"
                     /inference="COORDINATES: protein motif:HMM:NF014048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="endonuclease NucS"
                     /protein_id="HRE03244.1"
                     /translation="SYVSMLGRVEAAYGDLDTAFSEDRFAALLGDLTYSTADSRAAVP
                     NPSRITINGDLYSGLTSLRSAVSKYRAFLDQRDGAVPDDSAPDGAASTAEVVDARTFG
                     LERDLQAALRQSIGQLEPGLTIVDGGAEHSVASGRIDILASDAAGDLVVIELKAVRAG
                     RDAVGQILSYMGDIAAETGKPVRGLLVAPDFDHRALAALDVVDAVRAVRYEFTFLFGA
                     VQRGG"
     assembly_gap    1992..2048
                     /estimated_length=57
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          395 a          860 c          624 g          311 t
ORIGIN      
        1 cggcgtgctc gccacgctcg aaaccctcac ccgctccgag caggcggcgt cacgatgaac
       61 acgacagcca tcaccggcac cgtcaccacc agcccgtggc accggatccg ggcgttcgtg
      121 ccgaccacca ccttcgacgt tcgagtcgac ttcgcccgcc gcgcctacac gttccgtatc
      181 cgcgcgctca acgacctcgc ccgcaccgcc cagaccctcc aggtcggcga ccacgtcgcg
      241 atcaccggat acctgcacct cgaacccgcc agcaccgacc ccaccgcgct gcacttcgag
      301 atcgtcgcct acgaaatcga ccaccagccc ggccgccccg cagcgggcgg cgaccagtca
      361 cagacaagcg agaacgagcc gtgatcgccg accccgccac cgtcgtgctc accgcccacg
      421 tcgcccattg ggccgaccac caccccgccc gcaccgtctg gatcatcgac tgcctccacc
      481 gccacaacaa cggcgactgg ggcgacctcg accccgacga caccaacacc aaccgccacg
      541 ccctccgcca ccacgacggc cggatcctgt cccgctaccc gatccctacc gaactcgccg
      601 accccaccat cgacgacgac gcgatctgga tcatcaccga cgacctcgcg gaccccgaca
      661 ccctcaccac catcctctgg cccagcgact actgagcccg ggtgttcaag cgtgctcgct
      721 ccgagcacga tgacattgcg tgggcgacga cctgcgctgg cacgaggagc tgcgcgagct
      781 ctaccgccct cgacacctgc gcgtcttgct gatcggcgaa tcgccaccgg catccgacaa
      841 tagggcgcgt cggttcttct acgcaccgga gctcaccggg cacgacagct tgttccgatc
      901 ggccgcgctc gcggcctacg gctacacgaa ggccgacctc gctgaacggt cgaagacgag
      961 tgtcctgcta cgactccaag cggacgggat ctggctcatc gatgccgtcg agcatgcgat
     1021 caaccaccac gcggctaacg ctcggaccgc agcgatccgt nnnnnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnggcccg gagcacggag tgttcacttg ctcgaccccg gtacatcgcg
     1141 cggcggccgc gccgctggcc tcggctggca tcacggtctg caactccgaa cctgcgccgt
     1201 tccgctcggc aacatgagag cacggttcgt cagactctgg cggcgagaca tcccaggcag
     1261 ctggccgaaa tgaggtgcat ccgccgcgca cacccaggtg agcgcggcgc cggtcggtca
     1321 cccgccacgc tggaccgcgc cgaacaagaa cgtgaactcg tagcgaacag ctcgaactgc
     1381 gtcaacgaca tcgagcgccg ccaacgcccg gtggtcgaag tcgggtgcca ccagcagccc
     1441 gcgtaccggt ttgccggtct ccgccgcgat gtcgcccatg tacgacagga tctgaccgac
     1501 cgcatcacgg ccggcgcgca cagccttgag ctcaatcacc accagatcgc cggccgcgtc
     1561 gctcgccagg atgtcgatcc gacccgaggc caccgagtgc tcggccccgc cgtcgacgat
     1621 ggtcaaaccg ggttcgagct gaccgatcga ctgccgtagc gccgcctgca ggtcgcgctc
     1681 caacccgaac gtccgagcgt caaccacctc tgcggtgctc gcggccccgt ccggtgcgga
     1741 gtcgtccggc accgcaccgt cacgctgatc caggaacgcc cggtacttgc tcaccgccga
     1801 cctcaagctg gtcaaccccg agtacaggtc gccgttgatc gtgatccgcg aaggattggg
     1861 caccgcggcg cggctgtcgg ctgtcgagta ggtcaggtcg ccgagcagcg cagcaaagcg
     1921 atcctcgctg aacgccgtgt ccagatcacc gtaggccgcc tcgacgcgtc cgagcatcga
     1981 cacgtacgaa cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnncc cacatcggca gttcacaccg ccaactcgac cccgcgtact caagtcgccc
     2101 gcgttggtcg ctcacctgcc gccgccaacg gtcggttccg acgaacgggc agggcgtctt
     2161 tcacattcta ttacccgctg ggtggacagg gcttcccgta cgggggtgtg ctgacgatct
     2221 cgaagctgcg cgtcggccaa gaggcctacc agctgaccgg tgtcgcccgt tcactcggcg
     2281 a
//
LOCUS       DAORNF010000580         2279 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_35488_length_2279_cov_1.460432, whole genome shotgun sequence.
ACCESSION   DAORNF010000580 DAORNF010000000
VERSION     DAORNF010000580.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35488_length_2279_cov_1.460432"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..537
                     /locus_tag="PLV68_18250"
     CDS             <1..537
                     /locus_tag="PLV68_18250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03245.1"
                     /translation="PSPPCWLWGWPAGYPDGLTVTVGQIDNPYYLRLSQAIQDMVKDA
                     GFNFQLESVTGAEINNRLYQLKDLPVAITAFRGVADPGLTLEAKFASTGGSNPAGTTG
                     DGVDDLLAEGAASVDESVRAKAYQAVEKIVMEQALSVPIFHNGGLVAFVPEFKNVVKG
                     YTTCQFGDFVTAPVYFEK"
     gene            604..1359
                     /locus_tag="PLV68_18255"
     CDS             604..1359
                     /locus_tag="PLV68_18255"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HRE03246.1"
                     /translation="MILDSSAALADPRRRIAISRRILARNGCESMVAGHVSERSSDDA
                     GFWVSPFGYFDETTPEMVIKVDFDLNRLEGDWDPSPAIQFHAAIYRKRPDVGSVIHIH
                     SHYVSVFSTVGQPIGMYNVGSVLFYDDQVLHADDGSGPPVVGTTLAEELGDKRVVLIK
                     NHGAIVASQSLERATIEAMMLEVAARYHIEAQAIGGTEFPEAEVIRGRAAYHRHFLPN
                     MWDANVRRLQRSDPELFEATILPAGDDLVAHGR"
     gene            1349..>1953
                     /locus_tag="PLV68_18260"
     CDS             1349..>1953
                     /locus_tag="PLV68_18260"
                     /inference="COORDINATES: protein motif:HMM:NF015297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HRE03247.1"
                     /translation="MAAERPVPRIRSALFIPAGRADFLEKADRRGADAVILDLEDAVA
                     SPGKASARELASLWLRQRAADEAPYATVRVNAVGEGSIERDLDATVHPSLLAVLIPKV
                     TCADEILEVAELLSWFEGKRGMTRGAVRIWPIIETAEAVRDVSAIAAASPRVAFLGGG
                     TSEQGDLARNVGFEWSVDGMETLYIRSHVLIAARAAGIVNP"
     assembly_gap    1954..2057
                     /estimated_length=104
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2058..2260
                     /locus_tag="PLV68_18265"
     CDS             <2058..2260
                     /locus_tag="PLV68_18265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HRE03248.1"
                     /translation="HVAVVNEVFTPTAAQIDQATRVIAALDAAESSGIGAVVHDGKMI
                     DVAMARTAAALMADAEAIRRTG"
BASE COUNT          350 a          743 c          724 g          358 t
ORIGIN      
        1 ccatcgccac cctgctggct ttggggctgg ccggctggtt accccgacgg cctgaccgtc
       61 acggtcggcc agatcgacaa cccgtactac ctccgtctgt cgcaggcgat ccaggacatg
      121 gtgaaggatg ccgggttcaa cttccagctc gagtcggtca ccggtgcgga gatcaacaac
      181 cgcctgtacc agttgaagga tctgcctgtt gccatcaccg ccttccgcgg tgtggccgac
      241 ccgggtctga ccctcgaggc caagttcgcc tcgaccggtg gttcgaaccc ggccggcacg
      301 accggtgacg gggtcgacga tctgctcgcc gagggcgcgg ccagcgtcga cgagtccgtg
      361 cgggccaagg cctaccaggc ggtcgagaag atcgtcatgg agcaggcgct gtcggtgccg
      421 atcttccaca acggcggcct cgtcgctttc gtgccggagt tcaagaacgt cgtgaagggc
      481 tacaccacct gccagttcgg tgacttcgtg acggcccccg tctacttcga gaagtgaccc
      541 cacgcggttc gggctggatc acggtcccgc ccgaaccgct tctactttca atcggaggga
      601 accatgatcc tcgacagttc agccgctctc gccgatccgc gccggcggat cgcgatctcc
      661 cggcgcatcc tggcccgcaa cggttgcgag agcatggtgg ccggccacgt cagcgagcga
      721 tcgagcgacg acgccggatt ttgggtgtcg ccgttcggct acttcgacga gacgacgccc
      781 gagatggtga tcaaggtcga cttcgacctc aaccgcctcg aaggtgactg ggacccgtca
      841 ccggcgatcc agttccacgc cgcgatctac cgcaagcgac ccgacgtggg ctcggtgatc
      901 cacatccact ctcactacgt gtcggtcttc tccaccgtcg gccagccgat cggcatgtac
      961 aacgtcggct cggtgctgtt ctacgacgac caggtgctgc acgccgacga tggcagcggc
     1021 ccgccggtgg tcggcacgac gctggccgaa gagctcggcg acaagcgggt cgtgctcatc
     1081 aagaaccatg gcgcgatcgt ggcgtcgcag tcgctcgaac gggccacgat cgaagcgatg
     1141 atgctcgagg tcgcggctcg ctatcacatc gaggcgcagg ccatcggcgg caccgagttc
     1201 cccgaggccg aagtgatccg cggtcgcgcc gcgtaccacc gtcacttcct gccgaacatg
     1261 tgggatgcca acgtccgtcg tctgcagcgc agcgacccgg agctgttcga ggcaacgata
     1321 ttgccggccg gcgacgatct cgtggcccat ggccgctgag cggccagtcc cgcggattcg
     1381 cagcgcgctg ttcatccccg cgggccgcgc cgactttctc gaaaaggccg atcggcgcgg
     1441 tgccgacgcc gtcatcctcg acctggagga cgccgtcgca tcgccgggca aggcgtcggc
     1501 acgcgagctg gcgtcgctgt ggctgcgcca gcgcgccgcc gacgaggcgc cgtacgcgac
     1561 cgtgcgcgtg aacgcggtcg gcgaagggtc gatcgagcgc gacctcgacg ccacggtgca
     1621 cccgtccttg ctcgcggtgc tgatccccaa ggtcacctgc gccgacgaga tcctcgaggt
     1681 ggccgagctg ctgagctggt tcgagggcaa gcgcggcatg acccgcggtg ccgtgcggat
     1741 ctggccgatc atcgagacgg ccgaggctgt gcgcgacgtg tcggcgatcg ctgccgcgtc
     1801 gccgcgcgtc gcgttcttgg gtggtggcac gtccgagcag ggagatcttg cccgcaacgt
     1861 cgggttcgag tggagcgtcg acggcatgga gacgctgtac atccgcagcc acgtgctgat
     1921 cgccgcccgt gccgccggca tcgtcaaccc catnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnncgc acgtcgcggt cgtcaacgag gtgttcaccc ccaccgcagc
     2101 acagatcgac caagccaccc gcgtgatcgc cgcgctcgac gcggccgagt cgagtggcat
     2161 cggggcggtc gttcacgacg gcaagatgat cgatgtcgcg atggcgcgca cggcggctgc
     2221 cttgatggcc gatgccgagg cgatccgtcg cactggctga cgccgacacg agcatgaga
//
LOCUS       DAORNF010000581         2275 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_35569_length_2275_cov_1.557658, whole genome shotgun sequence.
ACCESSION   DAORNF010000581 DAORNF010000000
VERSION     DAORNF010000581.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35569_length_2275_cov_1.557658"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..689)
                     /locus_tag="PLV68_18270"
     CDS             complement(<1..689)
                     /locus_tag="PLV68_18270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03249.1"
                     /translation="MRAAVEQALADDGSTAASAAPLVTVEAFTPATAAVAVQVVVLGG
                     VVTGMVEGHVVQAEIPAAVVGALAADSRVDSLRSPRYVARPQTVTQPAVLPRTDVGPG
                     FGPVVTQSVALTNAATWQAAGIDGSVKIGIVDFFDFGLWSLAENGPLPDANHRFCRDS
                     SADPSLCDPGNNDGINNGDGYEHGVAVAQIVRDMAPAAELFLATAGTVSDLQAAIDWF
                     AANGGAIVNRSV"
     gene            complement(869..1414)
                     /locus_tag="PLV68_18275"
     CDS             complement(869..1414)
                     /locus_tag="PLV68_18275"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRE03250.1"
                     /translation="MPGAGEPRPQVAGDVRAAVAARVPLDERERRSIDAFLAHFDALS
                     SPFDEDADPVHVTASAIVIGARGVVLHKHKRLGLWLQPGGHIDAGELPWDAAVREAQE
                     ETGLAIEHADQRLAHVDVHPGPRGHTHLDLRYVITAADADPAPPPGESQEVRWFGWDE
                     AIAIADDGLRGALVALRGEAR"
     assembly_gap    1533..1542
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1547..>2275
                     /locus_tag="PLV68_18280"
     CDS             1547..>2275
                     /locus_tag="PLV68_18280"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRE03251.1"
                     /translation="MAEPDAQLAPRVLIAVGDPLLRRVASTGLRDRGFSVSATNEESA
                     ALVLARSFAPDVIIVDLSLDGSEATPLFDTLRQMSDCYMIGLAPTATEAERVRALRAG
                     GDDALSLPVSADELAARCQAMLRRPRQLHSRWDPMAASLVNIGPLQVDLGRKELRVRG
                     HDVPVTRIEFALFEQLCRKPAEVCSRVHLLEQVWGPNWVGDTHVVDVHLSNLRRKLQR
                     HAPELRFIHTVRGIGFRLSNDLLRA"
BASE COUNT          336 a          848 c          747 g          334 t
ORIGIN      
        1 accgaacggt tgacgatcgc gcccccgttg gcggcgaacc agtcgatcgc tgcctgcagg
       61 tcgctgaccg tgcccgcggt ggccaggaac aactccgccg cgggggccat gtcgcgcacg
      121 atctgggcga cggcgacacc gtgctcgtag ccgtcgccgt tgttgatgcc gtcgttgttg
      181 cccgggtcgc acaggctggg atcggccgag ctgtcgcggc agaagcggtg gttcgcgtcg
      241 ggcaatgggc cattctcggc caacgaccac aggccgaaat cgaagaagtc gacgatgccg
      301 atcttgacgc tgccgtcgat gcccgccgcc tgccaggtcg cggcgttggt cagcgccacc
      361 gactgggtca ccaccggccc gaaccccggc cccacgtccg tcctcggcag caccgcgggt
      421 tgtgtcacgg tttgcgggcg ggcgacatag cgcggcgagc ggagggagtc gacgcggctg
      481 tcggcagcga gggcgcccac caccgcggcg ggtatctcgg cctgcacgac gtgaccctcg
      541 accatgccgg tgaccacccc gcccaacaca acgacctgca ccgcgaccgc cgcggttgcc
      601 ggcgtgaacg cctcgaccgt caccaacggg gccgcgctcg cggccgtcga accgtcgtcg
      661 gccagcgcct gttcgaccgc tgcccgcaag cgcacatcgt cgatcgccgc tgcgtcatcg
      721 ctcgcggccg tgacaggccc gggctcggca accgctctcg cagacccggc ggccaagccg
      781 gacacgacca gaccggccac cagcagacca gcgaccctcc cccgccaccg gacgggccgg
      841 acagaccggg cacggctcat cgggacgctc atcgcgcctc gcctcgcagc gcgacgagcg
      901 cgccgcgcaa accgtcgtcg gcgatcgcga tcgcctcgtc ccagccgaac cagcgcacct
      961 cctggctctc tcccggcggc ggtgccgggt cggcatcggc cgcagtgatc acatagcgca
     1021 ggtcgagatg ggtgtgcccg cgcgggccgg gatggacgtc gacatgggcc aggcgctgat
     1081 cggcgtgttc gatcgccagc ccggtctctt cctgcgcctc gcgcaccgcc gcgtcccacg
     1141 gcagctcccc agcgtcgatg tgaccacccg gctgcaacca caacccgagc cgcttgtgct
     1201 tgtgcagcac cacaccgcga gcgccgatca cgatcgccga tgcggtgacg tgcaccgggt
     1261 ccgcatcttc gtcgaaggga gacgagaggg catcgaagtg ggcgaggaac gcgtcgatcg
     1321 aacgccgctc acgttcgtcg agcggcaccc gcgcggcgac cgcagcccgt acgtcaccgg
     1381 ccacttgggg acgtggttct ccggcaccgg gcatcccgcc accgtaccgg ccggcgaagt
     1441 ttgttcttga gcggaaccca gacggtgccg atacctcctg cgtcactcac gatcgcgccg
     1501 tctcgacggt ccggtcgttc actccacggc ggnnnnnnnn nnccacatgg cagagccaga
     1561 cgcacaactt gcaccgagag tcctcatcgc ggttggggac ccactgctgc ggcgtgtcgc
     1621 ctccaccggc ttgcgcgacc gtggcttttc ggtcagcgcc accaacgagg aatctgcggc
     1681 gttggtgctg gcccgttcgt tcgccccaga cgtgatcatc gtcgacctca gcctcgacgg
     1741 ctcggaggcc acacccctgt tcgacacgct gcgccagatg agcgactgct acatgatcgg
     1801 cctcgccccg acggcgaccg aggccgagcg ggtccgcgcc ctgcgcgccg gtggcgacga
     1861 tgccctgtcg ctccccgtgt ctgccgacga gctggccgcg cgctgccagg cgatgctgcg
     1921 ccgtccccgc cagttgcact cacgctggga tccgatggcc gcgtcgctgg tcaacatcgg
     1981 ccccctgcaa gtcgatctcg gccgcaagga gctgcgggtc cgcggtcacg acgtccccgt
     2041 cacccggatc gagttcgcac tgttcgagca gctctgccgc aagccggccg aggtgtgctc
     2101 gcgggtgcac ctgctggagc aggtgtgggg gccgaactgg gtcggcgaca cccatgtggt
     2161 cgacgtgcat ctgtccaacc tgcgccgcaa gctgcagcgc cacgcgcccg aactgcgctt
     2221 catccacacg gtgcgcggca tcggcttccg gttgagcaac gatctgctcc gcgcc
//
LOCUS       DAORNF010000582         2273 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_35606_length_2273_cov_2.157349, whole genome shotgun sequence.
ACCESSION   DAORNF010000582 DAORNF010000000
VERSION     DAORNF010000582.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2273
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35606_length_2273_cov_2.157349"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2273)
                     /locus_tag="PLV68_18285"
     CDS             complement(<1..>2273)
                     /locus_tag="PLV68_18285"
                     /inference="COORDINATES: protein motif:HMM:NF014396.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016158 - 3-phytase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phytase"
                     /protein_id="HRE03252.1"
                     /translation="PLPHASPALRVTRNRLQSFTGASSATGTVTPHKAATERGGSRWR
                     AGRDGGAGRRGATRLPTQEPRPLCRRSCTPTPMPSPDQSPDWYKRGNSGMPASSEINV
                     DRARVADIDGRADFLGAVILPTRFSFEGTQVGGLSGIVYDAANERFLAISDDRSQIDP
                     ARFYELDIDLTDGVLQGVRFVDVTTLRQADGTEFPALSLDPEAIALTGEGKLVISSEG
                     DASRLINPFVNVFAQDGLQIGSLPIPAAFLPVAGNTSGIRNNLAFESLTTTPDGKFLF
                     TAVENALVQDGPAASLADESPVRIVKYDLQTGQIVGEFIYATDPVVASPTPAGQFATN
                     GLVELLAIERSFSTGVGNAIRLYEVHTQGATDVSGFDAVGGFDIDAIADKRLLLDFAD
                     LGITLDNIEGMTLGPVLEDGRQSLILVSDNNFSGTQVTQVIAIGIDVEAIPSVAPVIE
                     TPAADRIDIDDLDARGRDLDDPAIYLNATDPAKSFVITTEKDAGFTSYDLNGTEIESS
                     PDAAIRYNNVDLIYGFELDGTAVDLAVFSDRENDTLAVYRIDAATQTLVDVTSAELLD
                     SAFTIFGEDDGEATAYGLATYKSIKDGKSYAYVTQADGAQVAQLELIDNGDGTVTAEV
                     VRTILLPVPAGDDAEDYQSEGIVVDQETGRLYVVPEGEIGILRYSAEADGGQDYAVVQ
                     PIDQAFFTPDVEGLSIYYGEDGEGYLIASSQGDNTFAVFSRTGNNAYLGSFVVGSEGG
                     IDGVQETDGIDIFSGPL"
BASE COUNT          349 a          715 c          788 g          421 t
ORIGIN      
        1 cagcgggccg ctgaagatgt cgatgccgtc ggtttcctgc acgccgtcga taccgccttc
       61 gctgccgacc acgaacgagc cgaggtaggc gttgttgccg gtgcggctga acacggcaaa
      121 ggtgttgtcg ccctggctgg aggcgatcag atagccctcg ccatcctcgc cgtaatagat
      181 gctgagcccc tcgacgtcgg gggtgaagaa cgcctggtcg atcggctgca ccacggcgta
      241 gtcctgcccg ccgtcggctt ccgccgagta gcgcaggatg ccgatttcgc cttccggcac
      301 gacgtagagc cggccggtct cctggtcgac gacgatgcct tccgactggt agtcttccgc
      361 gtcgtcaccg gcgggcaccg gcagcaagat ggtgcgcacc acttcggcag tgaccgtgcc
      421 gtcgccgttg tcgatcagct cgagctgggc gacctgtgcg ccgtcggcct gggtgacgta
      481 agcgtaggac ttgccgtcct tgatgctctt gtaggtggcg agaccgtagg cggtcgcttc
      541 gccgtcgtct tcgccgaaga tggtgaaggc gctgtccagc agttcggcgg aggtgacgtc
      601 gaccagagtc tgcgtcgccg cgtcgatgcg gtacacggcg agcgtgtcgt tctcccggtc
      661 ggagaacacg gcgaggtcga ccgccgtgcc gtcgagctcg aaaccgtaga tcaggtcgac
      721 gttgttgtag cggatggccg cgtccggcga cgattcgatc tcggtgccgt tgaggtcgta
      781 gctggtgaag cctgcgtcct tctcggtggt gatgacgaag ctcttggccg gatcggtagc
      841 attcaggtag atcgccggat cgtccagatc gcgcccgcgc gcatcgaggt cgtcgatgtc
      901 gatgcggtcg gcagccggcg tctcgatgac gggcgcaacc gacgggatcg cctcgacgtc
      961 gatgccgatc gcgatcacct gggtcacctg ggtgccgctg aagttgttgt cgctgaccag
     1021 gatcaacgac tgccgaccgt cctccagcac cgggcccagc gtcatgccct cgatgttgtc
     1081 gagggtgatg ccgaggtcgg cgaagtcgag cagcagacgc ttgtcggcga tggcgtcgat
     1141 gtcgaagccg ccaaccgcgt cgaagccgga gacgtcggtc gctccttgcg tgtgcacctc
     1201 gtagaggcgg atcgcattgc cgacgccggt cgagaacgac cgctcgatcg ccagcagctc
     1261 gaccaggccg ttggtggcga actggcctgc cggtgtcggg ctggccacga ccgggtcggt
     1321 cgcatagatg aactcgccga cgatctggcc ggtctgcagg tcgtacttga cgatccgcac
     1381 cgggctctcg tccgccagcg aggcggcggg accgtcctgc accagcgcgt tttcaacggc
     1441 ggtgaacagg aacttgccgt ccggcgtggt ggtgaggctc tcgaaggcga gattgttgcg
     1501 gatcccggag gtgttgccgg cgaccggcag gaacgcagcc gggatcggca ggctgccgat
     1561 ctgcagaccg tcctgcgcga aaacgttgac gaacgggttg atcagccggc tcgcgtcccc
     1621 ttccgaagaa atgaccagct taccttcgcc ggtcagcgcg atcgcctccg gatcgaggct
     1681 cagcgccggg aattccgtgc cgtccgcctg gcggagcgtg gttacgtcga cgaagcggac
     1741 gccctgcagc acgccatcgg tgagatcgat gtcgagttcg tagaagcggg ccgggtcgat
     1801 ctggctgcgg tcatcggaga tcgccaggaa gcgctcgttc gccgcgtcgt agacgatgcc
     1861 cgacaggccg ccgacctgcg tgccctcgaa gctgaaacgg gttggcagga ttactgcgcc
     1921 caaaaagtcc gcgcgtccgt cgatgtcggc gaccctggcg cggtccacgt tgatttctga
     1981 cgaagccggc atgccgctat tccctcgctt gtaccagtcc ggtgattgat caggagacgg
     2041 catgggcgtg ggcgtgcagc ttctccggca caatggtcgc ggctcttgag tcggcaggcg
     2101 cgtcgcgccc cgccgcccgg cccctccatc cctgccggcg cgccacctgc tcccgccgcg
     2161 ttccgtcgcc gctttgtggg gggtgaccgt acctgttgct gatgacgcgc cggtgaagct
     2221 ttgaagtcga ttgcgcgtaa cgcgcagcgc aggagaagcg tgcggcagcg gcg
//
LOCUS       DAORNF010000583         2269 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_35685_length_2269_cov_1.591689, whole genome shotgun sequence.
ACCESSION   DAORNF010000583 DAORNF010000000
VERSION     DAORNF010000583.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35685_length_2269_cov_1.591689"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(282..>1680)
                     /locus_tag="PLV68_18290"
     CDS             complement(282..>1680)
                     /locus_tag="PLV68_18290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HRE03253.1"
                     /translation="ELEQKRGISITSAVMQFEYRDCVVNLLDTPGHRDFSEDTYRVLA
                     AVDAVVMVLDSAKGIEPQTLKLFEVCRARRLPVLTFLNKFDRPVRDPLELLDEIETKL
                     DLRPTPVTWPVGDAEDFRGVIDRRAGHFVRFTRTARGSTIAPEETLSFDQAEADEGGL
                     WRKAIDGVGLLDAIGADLDLDSFLAGDSTPVFVGSALSAFGVRALLDAVVDLVPPPAP
                     RVDVAGHPRPIGAPCSAFVFKVQANMDRAHRDRIAFVRICSGHFERGMTMTVARTGKP
                     FATKYASTVFGAERSTIDEAFPGDVVGLVNATGLNLGDTLYEDQPVTFPGIPRFAPEL
                     FTNARPADTGRVKQFRRGIEVLEEEGVVQVLRDPSMGDAALVLAAVGQLQFEVFAHRL
                     ESEFNAPVEMTGSPYQAIRGTDEASADRLRQIGGIRILNRADGSLVALFENQYRLQRL
                     LSDEPELTLRSLAID"
     assembly_gap    1681..1872
                     /estimated_length=192
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1963..>2269
                     /locus_tag="PLV68_18295"
     CDS             1963..>2269
                     /locus_tag="PLV68_18295"
                     /inference="COORDINATES: protein motif:HMM:NF013335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HRE03254.1"
                     /translation="MRTPMLIDTDCGIDDAVALWWAVNSSDIELVGCTTVHGNVGVDI
                     ATVNTCRILEASGHGDVPVAIGAADAWGEAPPLTPADFVHGADGVGNLGHPPPVKTAA
                     "
BASE COUNT          368 a          710 c          697 g          302 t
ORIGIN      
        1 caccaccccc ggtcggagac gacgaaccag ccagaagcga gcgagcaccc ggacagcacc
       61 ctgcccgggc atcgccgcgt ccaggcagtg acgctccgtc acctccacca acgagacggt
      121 cacgcgggtg agtccacccg agcaaaccgg gagaactggg tcgatcggtg cggtcgggtc
      181 ggttgccgtt gttcgccgca tcgactacca cccccggcca aagacaacga acacacccaa
      241 accagcgaac acgcgggcac tcccctgccg gggctgaggg gctagtcgat ggcaagcgag
      301 cggagggtca attcgggttc gtcgctgagg aggcgttgca ggcggtactg gttttcgaac
      361 agggcgacca ggctgccatc ggcgcggttg aggatgcgga tgccgccgat ctggcgcaag
      421 cgatcggcgc tggcctcgtc ggtaccgcgg atcgcctggt acggcgatcc ggtcatctcg
      481 accggggcat tgaactcact ctccaagcgg tgggcgaaga cctcgaactg cagttggccg
      541 accgccgcca gcaccagcgc ggcgtcgccc atcgacgggt cgcgcagcac ctgtacgaca
      601 ccctcttcct ccagcacctc gatgccgcga cggaactgct tgacccgccc ggtgtcggcc
      661 ggacgggcgt tggtgaacag ctcaggggca aagcgaggga tgccggggaa cgtcaccggc
      721 tggtcctcgt acagcgtgtc gcccaggttc aagccggtgg cgttgaccaa gccgacgaca
      781 tcgccaggaa acgcttcgtc gatcgtgctc cgctcggcac cgaaaaccgt ggacgcgtac
      841 ttggtggcga acggcttgcc ggtgcgggcg acggtcatgg tcatgccccg ttcgaagtgg
      901 ccactacaga tccgcacgaa cgcgatccga tcgcgatggg cgcgatccat gttggcctgc
      961 accttgaaca cgaacgccga acagggcgcg ccgatcgggc gcggatggcc ggccacatcg
     1021 acccgtggcg ccgggggcgg caccaggtcg accaccgcgt cgagcaacgc ccgcacgccg
     1081 aaggccgaca gggccgaacc gacgaagacc ggcgtcgagt cgccggcgag aaagctgtcg
     1141 agatcgaggt cggcgccgat cgcgtcgagc aacccgacgc cgtcgatcgc cttgcgccac
     1201 agcccaccct cgtcggcctc ggcttgatcg aagctcagcg tctcctccgg cgcgatcgtg
     1261 ctgccgcgtg cggtgcgggt gaagcgcacg aagtgtccag cgcggcggtc gatcacacca
     1321 cgaaagtctt cggcatcgcc gacgggccac gtcaccggcg tgggccgtag gtcgagcttg
     1381 gtctcgatct cgtcgagcag ttccaacggg tcgcgcaccg ggcgatcgaa cttgttgagg
     1441 aaagtcagca ccggcagccg acgtgcccgg cacacctcga acagcttcag ggtctgcggc
     1501 tcgatgccct tggccgagtc gagcaccatc accaccgcgt cgaccgcggc gagcacccgg
     1561 taggtgtctt cgctgaagtc gcggtgaccc ggggtgtcga gcaggttgac gacgcagtcg
     1621 cggtactcga actgcatcac cgccgaggtg atcgagatgc cgcgcttctg ttcgagctcc
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nngatgtcgg cgctaccggc accgcgacaa cctcggcatc gggacagcct
     1921 tgggactgtg ccacgccggg tctggccgac gagactgaca cgatgcgcac acccatgctg
     1981 atcgacaccg actgcggcat cgacgacgcc gtcgccttgt ggtgggcggt gaactcatcc
     2041 gacatcgagt tggtcggctg tacgaccgtg cacggcaacg tcggcgtcga catcgcgacc
     2101 gtcaacacct gccgcatcct ggaagcatcg ggccacggcg atgtgccggt agcgatcggc
     2161 gcggctgacg cgtggggcga ggcaccaccg ttgaccccgg ccgacttcgt ccacggtgcc
     2221 gacggcgtcg gcaacctcgg gcatccgccc ccggtcaaga cggccgccg
//
LOCUS       DAORNF010000584         2257 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_35932_length_2257_cov_1.835150, whole genome shotgun sequence.
ACCESSION   DAORNF010000584 DAORNF010000000
VERSION     DAORNF010000584.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35932_length_2257_cov_1.835150"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="PLV68_18300"
     CDS             complement(<1..403)
                     /locus_tag="PLV68_18300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03255.1"
                     /translation="MTDAVITPTNTFRLSRSQLEAGPDGGLAGTAVFATEVVASAASH
                     VTITLVQAASVLRWDLWVGPDGAVVHPVGDGPDDELTGAVVATGNEALLVAGFVTDAF
                     GQLAVGAASEPGATTTLTELADGVVGQPDGQR"
     gene            complement(400..810)
                     /locus_tag="PLV68_18305"
     CDS             complement(400..810)
                     /locus_tag="PLV68_18305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03256.1"
                     /translation="MSDRVRVREAADGLGWRTVTDDPERQLTIVAQATATGTGFVPNV
                     VVQVLPVEPDFDLAATVPTAAVVLAQSGLDAGGDRASIMLIEPSRGVCLTQQIEYRFR
                     HDRVTRLVASADTADWPGVAGDVQQLIAAMGAES"
     gene            complement(875..>1645)
                     /locus_tag="PLV68_18310"
     CDS             complement(875..>1645)
                     /locus_tag="PLV68_18310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03257.1"
                     /translation="NTGAITPRPIGQAGGGGGGGGGGGAGDDEDSGAWDRVQSILEGL
                     GLSQGAIEAIIEALRKSGKLPDEGLLAVLGNDQLMGFIKGAGVVLDIVGVIVDFATDY
                     IDHPNLPTDERIIHALYDTALRFAMSEGVEAGVTWLMAAAGSVVPVLGTAVGAAVGKV
                     VGWALGEAVEFGLDKLDEHTDFVDKYADTVVEGYRAAKQGAEWVSDRVDDIKDGAGKV
                     VDGAKDVAGKVGQGARDAAGKVGGGIKSGYQNTLGRIF"
     assembly_gap    1646..1655
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1656..1907)
                     /locus_tag="PLV68_18315"
     CDS             complement(<1656..1907)
                     /locus_tag="PLV68_18315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03258.1"
                     /translation="MWGADADALDAAASQLVSAADDLDAQAALLTSNLGAIKWIGDVA
                     IRFTDAWSSGHQPRLRSVSAYLRDNAERLQHEAAEQRRVS"
     assembly_gap    2034..2043
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          359 a          812 c          685 g          381 t
ORIGIN      
        1 gacgctggcc gtccggttgg ccgaccaccc catcagcgag ctcagtgagc gtggtcgtcg
       61 cgcccggctc gctcgccgca ccaacggcca gttgtccgaa cgcgtcggtg acgaacccgg
      121 ctaccaacaa cgcttcgttg cccgtggcaa cgaccgcacc ggtcagctcg tcgtcaggtc
      181 catcacccac gggatgcacg acggcaccat cggggccaac ccagaggtcc cagcgcaaca
      241 ccgacgctgc ttgcacgagg gtgatcgtca cgtgcgacgc ggcgctcgca acgacctccg
      301 tggcaaagac ggcagtcccg gccaaaccgc cgtctgggcc tgcctctagt tgtgaccgcg
      361 acagccggaa cgtattcgta ggggtgatca ctgcatcagt catgactctg ctcccatcgc
      421 tgcgatcagc tgttgcacgt ctcctgcgac gccgggccaa tcggcggtgt cggcggacgc
      481 aacgagacgg gtcacccggt cgtggcggaa tcgatactcg atctgctgcg tgaggcacac
      541 gcctcgcgac ggttcgatca acatgatcga ggcccgatcg ccaccggcat cgaggcccga
      601 ctgagcgagc acgacggccg cggtcggcac ggtcgcagcg agatcgaagt ctggctcgac
      661 cgggagcacc tggacgacca cgttgggcac gaaccccgtg cccgttgctg tggcctgggc
      721 gacgatcgtc agttgccgtt cggggtcgtc ggtgaccgtg cgccacccca acccgtcagc
      781 ggcttcgcgg acccgcactc ggtcgctcat cgcgtcctcc catcactcgt ggccggtcgg
      841 gccggcgtgg ttggcaacac tgggcaacgg cccatcagaa gatccttcca agcgtgttct
      901 ggtagccgct cttgatgccg ccgcccacct tgcccgccgc atccctggct ccttggccaa
      961 ccttgccggc cacatccttg gcaccgtcga cgaccttgcc ggcgccgtcc ttgatgtcgt
     1021 cgacgcggtc ggacacccat tcggctccct gcttcgcggc tcggtatccc tcgaccaccg
     1081 tgtcggcgta cttgtcgacg aagtcggtgt gctcgtcgag cttgtcgagc ccgaactcga
     1141 cggcctcgcc gagcgcccaa ccgacgacct tgccgactgc ggcaccgaca gccgtgccaa
     1201 gcactggcac cacggagccc gctgcggcca tcagccacgt cacaccggcc tcgactccct
     1261 cggacatcgc gaaccgcagc gccgtgtcgt acaaggcgtg gatgatccgc tcgtcggtgg
     1321 gaaggttcgg gtggtcgatg taatcggtcg cgaagtcgac gatcacgccg acgatgtcga
     1381 gcaccacgcc agcgcccttg atgaaaccca tcaactggtc gttgcccagc accgcgagca
     1441 aaccctcatc gggcagcttg ccgctcttgc gcaaggcctc gatgatggcc tcgatcgctc
     1501 cctggctgag gccgaggcct tcgaggatcg actgcacgcg gtcccatgca ccagagtcct
     1561 cgtcgtcgcc cgcaccgccg cctccgccgc cgcctccgcc accgccggct tggccgatcg
     1621 ggcggggggt gatggcgccg gtgttnnnnn nnnnngctca cccggcgttg ttcggccgcc
     1681 tcgtgctgga ggcgttctgc gttgtcacgc aggtacgccg acacagaccg caagcgcggt
     1741 tgatgtccgc tggaccaggc atcggtgaag cggatcgcta cgtcgccgat ccacttgatc
     1801 gcgccgaggt tcgacgtcaa caacgccgcc tgcgcgtcga gatcatccgc tgccgagacc
     1861 agctgcgaag cagcagcatc gagggcatcg gcatcggcac cccacatcgt ggccatcacg
     1921 tcaccccgcc gccgttggcc gccgacacag cctcttgctt gtcggcggcg atcgtcgccc
     1981 gcttggccgc gtcgacgatg tcctgcacca attcgctcag cgccgggcga tgcnnnnnnn
     2041 nnngggcgtg ccattcgtgc acgaaccgct cccggtcggg gccgacccag gggatcgact
     2101 cgaccagttg cgtcagcttc gcggtcgtcg ccgtgatctc cgtcgaacgc ttcttcagac
     2161 ccaacacgaa gttgcggatc gccggaacat caccacctac catccccatc gcctgctcct
     2221 ttcagtacgt acgtgtgtcg tggatggaca acgaacg
//
LOCUS       DAORNF010000585         2256 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_35953_length_2256_cov_1.477510, whole genome shotgun sequence.
ACCESSION   DAORNF010000585 DAORNF010000000
VERSION     DAORNF010000585.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35953_length_2256_cov_1.477510"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="PLV68_18320"
     CDS             <1..309
                     /locus_tag="PLV68_18320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase"
                     /protein_id="HRE03259.1"
                     /translation="SGGEWISSVELENELMAHPKVKEAAVIGVPSQRWGERPLACVVV
                     APGETMTEAEVIEYLAPKLAKWQVPDDVVFIDEVPKTSVGKFSKKDLRTKFSDFRSIH
                     "
     gene            389..883
                     /locus_tag="PLV68_18325"
     CDS             389..883
                     /locus_tag="PLV68_18325"
                     /inference="COORDINATES: protein motif:HMM:NF012355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HRE03260.1"
                     /translation="MAGCAADYDDTFGPPLVVSSTAPTASGTAASGTAAAPTTTPGPA
                     SGNPPPTTAPPTTATPVTAPRTGVVVRVIAQDNLFRAATTTVHVGDTVQFQNVGRNDH
                     DVIPIDDSDAWGVAAADFTPGDVYEHVFDEPGEYAYYCSIHATNTPGVSPLAAMVGTV
                     IVTD"
     gene            935..>2256
                     /locus_tag="PLV68_18330"
     CDS             935..>2256
                     /locus_tag="PLV68_18330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HRE03261.1"
                     /translation="MRLHIERRRPHRLTAAICVGALVIGAAACGSSDDKADTTTTASS
                     APSTTAATTTTTAAITLPETVPAATDTPTSPDPTTPPDDGGIINVPDDYATIQEAVDA
                     AQPGDLVLIAPGNYNEAVTVTTPELTIRGLDRNEVVLDGEFTLDNGIRVVGANGVAVE
                     NMTARNYTRNGFFWTGVTGYRGSYLTAFRNGDYGLYAFLSTSGQFDHSYGAGSPDAGF
                     YIGGCFPCDAVLSEYNGLGYSGTNSGGDLYIVNSIFRYNRAGIVPNSGSYEPCYPERN
                     TTIVGNLVYSNNQPDTPAIDVALLAMGNGILSAGGVGNVIERNLVYDHDKTGIGLVPF
                     LEEDPNDDQPAPDEWETPCAETLDDPLPDPATVPDVVLWDSHDNTVRDNVLEDNRVAD
                     IAVDSAGSDLSGLGNCFSGNTHTTSAPTALETLAPCGGTGSGDWTAGGL"
BASE COUNT          408 a          821 c          710 g          317 t
ORIGIN      
        1 agcggtggcg agtggatcag ctcggtcgag ctcgagaacg agttgatggc ccaccccaag
       61 gtcaaggagg ctgccgtcat cggcgtgccc agccagcgct ggggcgagcg tcccttggcc
      121 tgcgtggtgg tcgccccggg cgagacgatg accgaggccg aggtcatcga gtatctcgcg
      181 cccaagctgg ccaagtggca ggtgcccgac gacgtcgtgt tcatcgacga ggtccccaag
      241 acgtcggtcg gcaagttcag caagaaggac ctgcgtacga agttctccga cttccgttcc
      301 atccactgaa cgtcgcgccg agctcatgtc atcacgcctc cttcggttcg gcgcgcccgc
      361 gcttgccgtg acatgtggca tcgtcgcctt ggcggggtgc gccgccgact acgacgacac
      421 cttcgggccg ccgctcgtgg tgagcagcac cgctccgacc gcatccggta cggcagcatc
      481 cggtacggca gcagcaccga ccacgacacc tggtccggcg agcggcaacc cgccaccgac
      541 gacggcccca ccgacgactg caacacccgt caccgccccg cgcaccgggg tcgtggtgcg
      601 ggtgatcgcg caggacaacc tgttccgcgc cgcgaccacc accgtgcacg tcggcgacac
      661 ggtgcagttc cagaacgtgg gccgcaacga tcacgacgtg atccccatcg acgacagcga
      721 cgcgtggggc gtggccgcgg ccgatttcac gcctggcgac gtctacgagc acgtcttcga
      781 cgagccgggc gagtatgcct actactgctc gatccacgcc accaacaccc ccggggtctc
      841 gccgctggcg gccatggtgg gtaccgtgat cgtgaccgac tgagccagca cgatcgacga
      901 acacgacaga gaacagcaat acagggggac tttcatgaga ctgcacatcg aacgccgacg
      961 accgcaccgg ctgaccgccg cgatctgcgt cggcgcactg gtgatcggtg ccgcggcctg
     1021 cggcagtagc gacgacaagg ccgacaccac gaccaccgcg tcgagcgcgc cgtccaccac
     1081 cgcggccacc acgaccacaa ccgcggcgat caccctgccc gagacggtgc cggccgcgac
     1141 cgatacgccg acgtcgcccg atcccaccac gcccccggac gacgggggca tcatcaacgt
     1201 cccggacgac tacgcgacca tccaggaagc cgtcgacgcc gcccagccgg gtgacctcgt
     1261 gctgatcgcg cccggcaact acaacgaggc ggtcacggtg accacgcccg agctcaccat
     1321 ccgtgggctc gatcgcaacg aggtcgtgct cgacggcgag ttcacgctcg acaacggcat
     1381 ccgcgtggtc ggcgccaacg gtgtggcggt cgagaacatg accgcccgca actacacccg
     1441 caacggcttc ttctggacgg gcgtcacggg ataccgcggt tcgtacctga ccgcgttccg
     1501 caacggcgac tacggcctct acgcgttctt gtcgacgtcg ggtcagttcg accacagcta
     1561 cggcgcaggt agccccgatg cgggcttcta catcggcggc tgcttcccct gcgacgcggt
     1621 gctcagcgag tacaacggct tggggtactc gggtaccaac tctggcggcg acctgtacat
     1681 cgtcaactcg atcttccgct acaaccgggc cggcatcgtg cccaacagcg gcagctacga
     1741 gccgtgctac cccgagcgca acaccaccat cgtcggcaac ctggtgtact ccaacaacca
     1801 gcccgacacg cccgcgatcg acgtcgcctt gctggcgatg ggcaacggca tcctcagcgc
     1861 cggcggcgtc ggcaacgtga tcgagcgcaa cttggtgtac gaccacgaca agaccggcat
     1921 cggcctggtg ccgttcctcg aagaagaccc caacgacgac cagcctgcgc ccgacgagtg
     1981 ggagaccccc tgcgccgaga cgctcgacga cccgctgccc gatccggcga cggtgcccga
     2041 cgtggtgctg tgggacagcc acgacaacac ggtgcgcgac aacgtgttgg aggacaaccg
     2101 cgtcgctgac atcgccgtcg actcggccgg gagcgacctg tctggcctgg gcaactgctt
     2161 cagcggcaac acccacacca cctcggcccc gaccgcgctg gagaccctcg cgccgtgtgg
     2221 tggcaccggg tcgggcgact ggaccgcggg tggtct
//
LOCUS       DAORNF010000586         2253 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_36008_length_2253_cov_1.745223, whole genome shotgun sequence.
ACCESSION   DAORNF010000586 DAORNF010000000
VERSION     DAORNF010000586.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2253
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36008_length_2253_cov_1.745223"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..748
                     /locus_tag="PLV68_18335"
     CDS             <1..748
                     /locus_tag="PLV68_18335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03262.1"
                     /translation="SEKMARNVLVIAEAIAAEWNAQGSDIDRILGGYQDTVVQRLTSS
                     PETPSAPGKAGIGCGFGVLVLTLGIAALIVGIVGLVRANNSLRADAVAVGYAAPRGTQ
                     PDTHRFTADSDRSYSIYIDLDTSNENRRDSIVASVECQAAFQNGTTAHIRGSRQGTST
                     TIGDVSSIGKFDAPAGAVAVACYQVSNSGGGNSLLYFVTPGKSRFLVWILVTVGGGLL
                     IALGIVLGIMRLVRKPLAAARQMQASISAG"
     gene            complement(737..>1910)
                     /locus_tag="PLV68_18340"
     CDS             complement(737..>1910)
                     /locus_tag="PLV68_18340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03263.1"
                     /translation="AAFYADERLISNQTMALVDLTAIDELDAALAGLVDLSGANATAA
                     AVGLARSAARSYSFANSPDRTLDYNLFDIGDLVKTLAVQNPLLAPSVDAVLDALGAAV
                     VYDITGSLSTETHGLAIYLPPAADVADPRYFDLPGLSAWQSLVRSYLDTTSSVQVPTA
                     FTDDDKYIEGRWTSQGFEIGSTYDPAAEATVVDARIRYGTYWSDYTEMMFFGSEPLLF
                     GNGEVRGVWDPQMLTVTDGNSQGFAYATIDLSGDGKRAIFSIPLDYEGPNGRGRVDLR
                     IVVEIGGGVLSKEFVLYSDTGIGRAPIDPRGQLWAIALVQDLATFDQRWERTHAAPFA
                     ADIDALDVYYEYMSEATPILAQLELHALDGSVDFVYAGDAVPAFYYDERNTYPLPG"
     assembly_gap    1911..2044
                     /estimated_length=134
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2045..2225)
                     /locus_tag="PLV68_18345"
     CDS             complement(<2045..2225)
                     /locus_tag="PLV68_18345"
                     /inference="COORDINATES: protein motif:HMM:NF015380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clostripain-related cysteine peptidase"
                     /protein_id="HRE03264.1"
                     /translation="MIGDHGAGWHGVAWDDATGNHLNPADVRDAIATGLTAAGRERAD
                     IVGFDACLMATFEVGA"
BASE COUNT          354 a          711 c          718 g          336 t
ORIGIN      
        1 cagcgaaaag atggcgcgca acgtgctggt catcgccgaa gcgatcgccg ccgaatggaa
       61 tgcgcagggc agcgacatcg accgcatcct gggcggctac caggacacgg tggtgcagcg
      121 tctcaccagc tcgcccgaga cgccgagtgc tccgggcaag gcaggtatcg gatgcggttt
      181 cggcgtgctg gtgctgacgc tgggcatcgc cgcgctgatc gtcggcatcg tcgggctggt
      241 ccgcgccaac aactcgctgc gcgccgacgc ggtcgcagtc ggctacgccg cgccccgggg
      301 cacccaaccc gacacgcacc gcttcacggc ggactccgac aggtcgtaca gcatctacat
      361 cgacctcgac acgagcaatg agaacaggcg cgacagcatc gtcgcctccg tcgagtgcca
      421 agccgctttc cagaacggaa ccacagcgca tatccggggc agccgccagg gcacgtcgac
      481 cacgatcggc gacgtgtcct cgatcggcaa gttcgacgcc ccggcggggg cggttgccgt
      541 cgcctgctac caggtttcca actcaggcgg cggcaactcg ctgctgtact tcgtcacccc
      601 cggtaagtct cgcttcctgg tgtggattct ggtcaccgtc ggcggaggct tactgatcgc
      661 cctcggcatc gtgctgggca tcatgcgcct ggtccgcaag ccgctcgccg ccgcccgcca
      721 gatgcaggcc tcgatctcag ccgggtagcg ggtacgtgtt gcgctcgtcg taatagaagg
      781 cgggtactgc atcgcccgcg tagacgaagt cgaccgagcc atccagcgcg tgcagttcga
      841 gttgagccag gatcggcgtg gcctcgctca tgtactcgta gtagacgtcg agtgcgtcga
      901 tatcggcggc gaacggcgcg gcgtgggtgc gttcccaccg ttggtcgaac gtcgccaggt
      961 cctgcaccag cgcgatcgcc cacaactggc cgcgcgggtc gatcggcgcg cggccgatgc
     1021 ccgtgtcgga gtagagcacg aactccttcg acagcacacc gccgccgatc tccacgacga
     1081 tgcgcagatc gacccggccg cggccgttcg gtccttcgta gtcgagcggg atcgagaaga
     1141 tcgcccgctt gccgtcgcct gacaggtcga tggtggcgta ggcgaaaccc tgcgagttgc
     1201 cgtcggtcac cgtcagcatc tgcgggtccc acacgccccg gacctcaccg ttgccgaaga
     1261 gcaacggctc agagccgaag aacatcatct ccgtgtagtc gctccagtac gtgccgtacc
     1321 gaatgcgcgc gtcgaccacg gtcgcttcgg ccgcagggtc gtatgtggag ccgatctcga
     1381 agccctgcga cgtccagcgt ccctcgatgt acttgtcgtc gtcggtgaac gccgtcggca
     1441 cctgcaccga cgatgtggtg tcgaggtacg agcgcaccag cgattgccat gccgacagac
     1501 cgggcaggtc gaagtagcgc gggtcggcca cgtcggccgc cgggggcaag tagatcgcca
     1561 gaccgtgggt ctcggtcgac aacgagccgg tgatgtcgta gaccactgcc gcgccgagcg
     1621 cgtcgagcac ggcatcgacg ctgggggcca agagagggtt ctgcaccgcc aacgtcttga
     1681 ccaggtcgcc gatatcgaac aggttgtagt cgagcgtgcg gtcgggcgag ttggcgaagc
     1741 tgtacgagcg agccgcgcta cgggccaggc cgaccgcggc agcggtcgcg ttggcgccgg
     1801 acaggtcgac gagaccagcc aaggcggcgt cgagctcgtc gatcgcggtc aggtcgacca
     1861 gcgccatcgt ctggttggag atcagccgct cgtccgcata gaacgcggcg nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnccgcgc caacctcgaa ggtggccatc agacatgcgt cgaagcccac gatgtcggcc
     2101 cgctcccgac cagcggcggt cagcccggtg gcgatcgcgt cgcgcacgtc ggcggggttg
     2161 aggtggttgc cggtggcgtc gtcccacgcc acgccgtgcc agcccgcgcc gtggtcaccg
     2221 atcacgacgg cgatccgctc gcggggatag ttg
//
LOCUS       DAORNF010000587         2251 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_36053_length_2251_cov_1.678051, whole genome shotgun sequence.
ACCESSION   DAORNF010000587 DAORNF010000000
VERSION     DAORNF010000587.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36053_length_2251_cov_1.678051"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..719
                     /locus_tag="PLV68_18350"
     CDS             <1..719
                     /locus_tag="PLV68_18350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HRE03265.1"
                     /translation="PVTLPTATACTDRGGSGAGSGTQPVTCSECNGRGQVQRVRQSLL
                     GQMMTSSPCGRCGGTGQVIATPCGTCAGDGRVVTDKTYQVDVPGGVDTGSTLRLSGRG
                     AVGPRGGAAGDLYVHIRVAAHERFRREELDLVTDLPISIAQAALGTRFNLETLDGEED
                     LVVPAGTQPGREFVLRGRGVPRLQGRGRGDLRVRVQVEVPTKLSDDEVDLLRQFAEGR
                     GEYVESPDKGLFSRIKSAFS"
     gene            716..1429
                     /locus_tag="PLV68_18355"
     CDS             716..1429
                     /locus_tag="PLV68_18355"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HRE03266.1"
                     /translation="MNPALRRSSAHVFVEALTAPSLDERDRHHLLRVLRLRAGEAVTV
                     SDGAGAWRVCRLGPEGTLTPDGDIVVGHSRGDSPSVSLTVGVAIPKAERPEWIVQKLT
                     EIGVDHIVFVAAARSVVRWDAAKAARNLERLVAREAAMQSRRLTIPVVDGPLALSELL
                     AASSASAALALAEPGEADLDPSINGVLIGPEGGWSPAELDLVERRVGLGPTVLRVETA
                     ALVAATRLVQLHRGAGHAE"
     gene            1590..2111
                     /locus_tag="PLV68_18360"
     CDS             1590..2111
                     /locus_tag="PLV68_18360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HRE03267.1"
                     /translation="MREFDDDDVSSSPYSRKVGERLRVIRRQKRLSLQDVEASSRDEF
                     KASVLGAYERGERAISVPRLERLASFYNVPVDQLLPRDEQAMSHEALGDMAAARKMAV
                     DLVKLSQLSGQPFEMLARFLRMIQVQRQDFNGKVLTVRGDDTRAIAAMLDVPVDQVAQ
                     RLDALDLLYRPPG"
BASE COUNT          333 a          759 c          797 g          362 t
ORIGIN      
        1 tgccggtcac gctgcccacc gccacggcct gcaccgaccg cggtggcagc ggcgccggca
       61 gcggtaccca gccggtcacg tgcagcgagt gcaacgggcg gggtcaggtg cagcgtgtcc
      121 gccagagcct gctcggccag atgatgacca gctcgccgtg cgggcgttgc ggcggaaccg
      181 ggcaggtgat cgccacaccg tgcggcacct gtgcgggcga cggccgggtc gtcaccgaca
      241 agacctacca ggtcgacgtg cccggcggag tcgacacggg atccacgctg cgcctcagcg
      301 gtcgcggcgc ggtcggcccg cgcggcgggg ccgcaggaga tctgtacgtg cacatccgag
      361 tcgcggcgca cgaacggttc cgccgcgaag aactcgatct ggtcaccgat ctgccgatct
      421 cgatcgccca agcagcgctg ggcacccgct tcaacctgga gacgctcgac ggcgaggaag
      481 acctcgtcgt accggcagga acccaaccgg gccgcgagtt cgtgttgcgc ggccgtggcg
      541 tgccccgctt gcaagggcgc ggccggggcg acctgcgcgt ccgggtgcag gtcgaggtgc
      601 ccaccaagct cagcgacgac gaggtcgacc tgctccgcca gttcgccgag ggccgtggcg
      661 agtacgtcga gtcgcccgac aagggcctgt tctcgcggat caagtccgca ttctcgtgaa
      721 cccggcgctg cgccggtcga gtgcgcacgt cttcgtcgag gcgctcaccg cgccgagcct
      781 cgacgagcgt gatcggcacc acctgttgcg ggtgctgcgc ctgcgcgccg gcgaggcggt
      841 cacggtcagc gacggtgccg gtgcgtggcg ggtgtgccgg ctcggccccg agggcacact
      901 gacgcccgac ggcgacatcg tcgtcggcca ctcacgcggt gactcgccat cggtcagcct
      961 gacggtcggg gtcgccatcc ccaaggcaga gcggcccgag tggatcgtgc agaagctgac
     1021 cgagatcggc gtcgatcaca tcgtgttcgt cgccgcggcg cgcagcgtgg tgcgctggga
     1081 tgcggccaag gcggcgcgca acctggaacg gctcgtggcg cgcgaggccg cgatgcagtc
     1141 gcggcggttg acgatccccg tggtggacgg tccgctcgcg ttgtccgagt tgctggctgc
     1201 atcgtcggcg agcgcggcgt tggcgttggc cgaaccgggg gaggccgatc tcgatccgtc
     1261 gatcaacggc gtgttgatcg gtcccgaagg gggctggtcg ccagccgaac tcgatctggt
     1321 cgagcgccgt gttggccttg gccccactgt gctgcgggtc gagaccgcag cactggtggc
     1381 cgcaacccgg ctcgtgcagc tgcaccgcgg cgctggccac gccgagtgag acactggccg
     1441 cggtcgtcac atctgcagac gtcacatatt gggctcaagt ttgttcagga agtggccgat
     1501 tcaacactca ccgttgccag tcaacgcgga gtgtggttga attgccactt ccgagccgac
     1561 gcgttccctg gcgggaagag gagaatcaga tgcgcgagtt cgatgacgac gacgtctcgt
     1621 cgtcccccta cagccgcaag gttggcgagc gtttgcgcgt catccgtcgc cagaagcggc
     1681 tgagcttgca agacgtcgag gccagctcgc gcgacgagtt caaggccagc gtgctcggtg
     1741 cttacgagcg cggcgagcgg gcgatctcgg tccctcgcct ggaacggttg gcgtcgttct
     1801 acaacgtgcc ggtcgatcag ttgttgcccc gcgacgagca ggcgatgagc cacgaggcgc
     1861 ttggcgacat ggccgcggcg cgcaagatgg ccgtcgacct ggtcaagctg tcgcagctct
     1921 cgggccaacc gttcgagatg ctggcccgtt tcttgcggat gatccaggtg caacggcagg
     1981 acttcaacgg caaggtgctc accgtgcgcg gtgacgacac ccgcgcgatc gcggcgatgc
     2041 tcgacgtgcc cgtcgaccag gtcgcgcagc gcctcgacgc gctcgacctg ctctaccgcc
     2101 caccgggctg agccgctcga ctctcccgca gtcgaccacc cgcactcgta tcccgtccgg
     2161 actcgtatcg tggtcgccgc gatgcgtgag ccgttcggtg tctatctcca cattccgttc
     2221 tgcgcgcacc ggtgcgacta ctgcgcgttc g
//
LOCUS       DAORNF010000588         2249 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_36078_length_2249_cov_1.868277, whole genome shotgun sequence.
ACCESSION   DAORNF010000588 DAORNF010000000
VERSION     DAORNF010000588.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36078_length_2249_cov_1.868277"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..381)
                     /locus_tag="PLV68_18365"
     CDS             complement(<1..381)
                     /locus_tag="PLV68_18365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03268.1"
                     /translation="MFVGPVTPQDVVIAAHTLKHSDWQMCEALGLLHAAVDVTRRADR
                     SGLSPNVLGDTLLRVQREVEALQTVVATLAFDVEQSGLAGADGSRDAADWFAKKGKVT
                     PNKAKNAIKLGGALDRSQDLQDALD"
     gene            748..>1171
                     /locus_tag="PLV68_18370"
     CDS             748..>1171
                     /locus_tag="PLV68_18370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03269.1"
                     /translation="MLSSFRRVKKGPGHRPQSAKRRRFMQLRERGLSIDAATREVGAS
                     RTAGRNWANGKPPRQHRAVFDRHRQRRAETGIAGKLKGHEALTACLPGIYARTMNARG
                     QPTCWRLGEPLNGTQSASRVGGSGTSYKVIQPDRLGKGS"
     assembly_gap    1172..1203
                     /estimated_length=32
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1400..1741)
                     /locus_tag="PLV68_18375"
     CDS             complement(1400..1741)
                     /locus_tag="PLV68_18375"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HRE03270.1"
                     /translation="MPTSGDVVDIDLGAPEGREAGFVHPAVVVTAQRILDAGPSVVHV
                     VPLTSTTRGFHSELVIGPVARNGLDVASAAQCQHLRSISPNRIRSRRGNVGTVALTHI
                     RETLAASLDLP"
     gene            complement(1725..1919)
                     /locus_tag="PLV68_18380"
     CDS             complement(1725..1919)
                     /locus_tag="PLV68_18380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03271.1"
                     /translation="MQSTSVRIDVATHEDLKRLAAELDTTVGNTVTLAVRALRQDRIG
                     ADLRAPLRDDEVPWLDADFG"
BASE COUNT          405 a          716 c          732 g          364 t
ORIGIN      
        1 atcgagggcg tcctgcagat cttgggacct gtcgagcgcg ccacccaact tgatcgcgtt
       61 cttcgccttg ttcggggtga ccttgccttt cttggcgaac cagtccgccg cgtcccgcga
      121 accatccgcg ccggcgaggc cggactgttc gacatcgaac gccaacgtcg ccaccaccgt
      181 ctgcaacgcc tcgacctcgc gctgcacccg caacagcgtg tcacccaaca cgttgggtga
      241 cagaccggac cggtccgcgc gccgggtgac gtcgaccgcg gcgtgcaaca accccaacgc
      301 ttcgcacatc tgccaatcgg agtgtttcag ggtgtgcgcc gcgatcacca catcctgtgg
      361 agtcaccggt ccgacgaaca tacgagaacc atacacatgt tcgtgtcggg atgcaacccg
      421 aatctcatca caattcgaga tactgcgcct ggttgatatc gccgccgcag atacccctac
      481 acgtcacgcg ctctgtcagc acgcgatggc acattcgtgc actccacggc atcgctcagt
      541 ccggcgggta tcagttgtgg tgacgcccga cgcggcagcg cgtgcagcgc cacccggccc
      601 ctgggctctc cacacaagta cgtgactggt cgccaccctg caacggcgca gtcggtggca
      661 ccacctgtcg gtgacggctg aaaactgcgc agtcctgccg cggtggtttc cagtgatggt
      721 cgcaacactt ccgccaatct ggaggtcatg ttgtcatcgt ttcgtcgggt caagaagggg
      781 ccggggcatc gcccgcaatc ggccaaacgt cgcaggttca tgcaactgcg agaacgcgga
      841 ctgagcatcg acgcggcaac ccgcgaggtc ggcgcgtcac gcaccgctgg gaggaactgg
      901 gcgaacggga aaccaccgcg gcaacatcgc gcagttttcg accgtcaccg acagcggcgg
      961 gcggagaccg ggattgctgg caagctcaaa ggacacgagg cgctcaccgc atgtttgcct
     1021 ggcatctatg cgcggaccat gaacgcgcgg ggccaaccca catgctggag gctgggcgag
     1081 cctttgaatg ggacgcaaag cgcctcgcgg gtcggcggat cgggcacttc gtacaaggtc
     1141 attcagcccg atcgactcgg caagggctcc gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnggtggca agcgggcgtg ttcgacgcgc tggtcgacgg accgactgac cggggcgaat
     1261 gcgggtgacg tggtcgctgg gcaccgaacg caacggggtc ccgctcggct cggagatcgc
     1321 cgacgccaaa ccgcaacgac cgcgtcgcca aactcaccaa ccactggacg ccagaatcag
     1381 gacctactgg cgctcgctct cacgggaggt cgaggctggc ggcgagggtc tcgcggatgt
     1441 gggtgagggc gaccgtgccg acgttgccgc gccgcgatcg gatgcgattc ggtgagatgg
     1501 agcgcaggtg ttggcattgg gcggcagatg cgacgtcgag gccgttgcgg gcgacgggtc
     1561 cgatgaccaa ctcggaatgg aaaccgcggg ttgtcgacgt cagcggcacc acgtgcacga
     1621 ccgacgggcc agcgtcgagt atccgttgag cggtcaccac gaccgcaggg tggacgaagc
     1681 ccgcttcgcg accctcggga gcgccgagat cgatgtcgac gacgtcaccc gaagtcggca
     1741 tcgagccacg gcacctcgtc gtcacgcaat ggcgcgcgca ggtcggcccc gatccggtcc
     1801 tggcgcaacg cccgcacggc gagggtgacg gtgttcccga ccgtcgtatc gagttcggca
     1861 gccagtcgct tcaggtcctc gtgggtcgca acatcgatcc gaacactcgt gctctgcatg
     1921 cacatcagca tgcatcgtct cggcaccgcc tggccacagc cttcaggtgg cgtcactaca
     1981 tggtggcgtc aagcaggtcg ccgacgtgat tggcttccca cacgtccgtt atggcctcga
     2041 tgtcgggata ggggccggga gccgaccagg atcgagtcgt cgaagcgaac cttgccctgc
     2101 agcaagccga acacccgcgt gggcttgggc tgttcgaaca tgtcgtcagc gtcattactg
     2161 agctgagctc aagccgggcc gaggggcttc ggtgcatcgt tgtcggggcc gcagcgatgt
     2221 gaacaggccg ggtggaatgg ggcggggga
//
LOCUS       DAORNF010000589         2247 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_36115_length_2247_cov_2.049726, whole genome shotgun sequence.
ACCESSION   DAORNF010000589 DAORNF010000000
VERSION     DAORNF010000589.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36115_length_2247_cov_2.049726"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /locus_tag="PLV68_18385"
     CDS             <1..353
                     /locus_tag="PLV68_18385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03272.1"
                     /translation="ADLVVRRILRIKERPAGVSSKQAYAAFQKSMLISATRCTLTYVV
                     FPFLIPAIGVATGVGPVLGIIIGAVAMVCDVFTIRRFFSVDHKWRWHFSAVALCVVCL
                     LMVLMVQDISHLIS"
     gene            564..1238
                     /locus_tag="PLV68_18390"
     CDS             564..1238
                     /locus_tag="PLV68_18390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRE03273.1"
                     /translation="MATRVLVVEDDADVRTMLRMVLQDEEFDVVEADCGEAGLTLADS
                     TIDLVLLDLKLPDISGFEVCRQLRQRGDMPIVMVTAQIDSYDMVAGLSAGADDYVTKP
                     FIPKVLVARIRALLRRVHGGTETGRIRCAGLEIAPLEGLAARDGVPIALTKTEFRLLC
                     DLAQHANRVISRELLLQRVWGYEFPGDGRLVDSHIRRLRTKVELDPANPALIQTIRGF
                     GYKFVC"
     gene            1286..>2247
                     /locus_tag="PLV68_18395"
     CDS             1286..>2247
                     /locus_tag="PLV68_18395"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HRE03274.1"
                     /translation="MRARITVTFAVLTMVATLAVSVSTFVLARGYLIDQRERSAVRQT
                     FLNARLARDLLDSGDRQPQDVLDVTVGEVGTLALLRVHGQWYASGVSADPAVLPPDLL
                     AALESGAAAHQRQSRARTPRLVTGVPLGDSDTLYIEIVSLSGLQNTLRTLVISLLIGS
                     AGTTVVGAIVGMYVSRRVLRPLTRMSGVAAGITGGDTEARLDAQGDSDLGPLVASFNE
                     MVDALQTQIERERRFASDVSHEMRTPLTVLKASVQLVEQRAPDLTPRAMEAVTMMSRQ
                     VAYFERLVLDLLEISRLDAGVDEPNLEETDVVAFVARTVADAGGP"
BASE COUNT          331 a          757 c          759 g          400 t
ORIGIN      
        1 aagccgacct ggtggtgcgg cgcatcctgc gcatcaagga acgcccggcc ggggtcagct
       61 ccaaacaggc ctacgccgcg ttccagaagt cgatgctgat ctcggcgacg cgctgcacgc
      121 tcacctacgt ggtcttcccg ttcctgatcc cagcgatcgg cgtcgccacc ggcgtcgggc
      181 cggtgctggg catcatcatc ggtgccgtgg cgatggtctg cgacgtgttc accattcgcc
      241 gtttcttcag cgtcgatcac aagtggcggt ggcacttctc cgccgtcgcg ctgtgcgtgg
      301 tctgcctgct gatggtgctg atggtgcaag acatcagcca cctgatcagc tgaccggcac
      361 cgttctggcc gttctggccg ttcgagcgtt ggcgttctgg ccgagtccgg ggcggacaca
      421 gcgccaccgc gggaacgccc cggactcgtg ggcgccatct tcggtgcgcc gtgtgacaga
      481 cagcccggat ttgtgttgtg gttctgtgga gattgcccag agcccgtcgg tggcaccggt
      541 ggcatgcatg gcatgatcgc gacatggcca cacgagttct cgtcgtcgaa gacgacgccg
      601 acgtgcgaac catgttgcgc atggtgctgc aggacgagga attcgacgtc gtcgaagccg
      661 actgtggcga agcgggcctg acgttggccg acagcacgat cgacctggtg ctgctcgacc
      721 tgaagctgcc cgacatcagt ggcttcgagg tctgccgcca actgcgtcag cggggcgaca
      781 tgccgatcgt gatggtcacc gcccagatcg acagctacga catggtcgcg gggttgtctg
      841 ccggtgccga cgactacgtc accaagccgt tcatccccaa ggtgctcgtc gcccgcatcc
      901 gcgccctgct gcgccgggtg cacggcggga ccgagaccgg ccgcatccgc tgcgcaggcc
      961 tggagatcgc cccgctcgaa ggtctcgccg cccgcgacgg cgtgccgatc gcgctgacca
     1021 agaccgagtt ccgcctgctg tgcgacctgg cccagcatgc caatcgggtc atctcgcgcg
     1081 agttgctact gcagcgcgtg tggggctacg agttccccgg cgacggccgt ctggtcgact
     1141 cccacatccg ccggttgcgc accaaggtcg agctcgaccc ggccaacccc gcgctgatcc
     1201 agacgatccg cggcttcggg tacaagttcg tgtgctgaac cgacgcctct cagaacgggg
     1261 tcggcgaacg tcacgcccgc tgccgttgcg ggcccggatc accgtcacct tcgctgtgct
     1321 gaccatggtc gccaccttgg cggtctcggt gtcgacgttc gtgctggcgc ggggctacct
     1381 catcgaccag cgcgagcgca gcgcggtgcg tcagaccttc ctcaacgccc ggctggcccg
     1441 cgacctgctc gactccggcg accgtcagcc gcaggacgtg ctcgacgtga ccgtcggtga
     1501 ggtgggcacg ctggcgctgc tgcgcgtgca cgggcagtgg tacgcatcgg gcgtgtctgc
     1561 cgacccggcg gtgttgcccc ccgatctgct cgctgcgctg gagtctggag cagcggccca
     1621 tcagcgccag tcgcgcgccc gcacgccacg gttggtcacg ggggtgcccc tcggcgactc
     1681 cgacacgctg tacatcgaga tcgtgtcgct gagcgggttg cagaacaccc tgcgcacgct
     1741 ggtgatctcg ctgctgatcg gttcggccgg aaccacggtg gtcggtgcga tcgtgggcat
     1801 gtacgtcagc cgccgcgtgc tgcgcccgct gacgcgcatg tcgggtgtcg cggccggtat
     1861 cactgggggc gacaccgagg cccggctcga cgcacagggc gactccgatc tcggcccgct
     1921 ggtcgcttcg ttcaacgaga tggtcgatgc gctgcaaacg cagatcgagc gcgaacggcg
     1981 gttcgcgtcc gacgtcagcc acgagatgcg cacaccgctg acggtgctga aagcatcggt
     2041 gcagctggtc gaacaacgcg cccccgactt gacgccgcgg gcgatggagg cggtcacgat
     2101 gatgtcccgc caagtggcgt acttcgaacg cctcgtgctc gatctgctcg agatctctcg
     2161 gctcgacgcg ggtgtggacg aaccgaacct ggaggagacc gacgtcgttg cattcgtggc
     2221 ccgaacggtt gccgatgccg gtggacc
//
LOCUS       DAORNF010000590         2246 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_36141_length_2246_cov_1.811958, whole genome shotgun sequence.
ACCESSION   DAORNF010000590 DAORNF010000000
VERSION     DAORNF010000590.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36141_length_2246_cov_1.811958"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>775
                     /locus_tag="PLV68_18400"
     CDS             <1..>775
                     /locus_tag="PLV68_18400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03275.1"
                     /translation="AGVVDLPVYAGEFKVVCAKTLGYGLGIVPPADGGSVEIHLSRDH
                     PTQDVTVYQTGRVGDVWALHDYGSNGPPFISVAPMSGEFGPDPFPLTFSLDCIDDETV
                     DSTYYQTITGGGAHPARIDVYYDCRPDYFTVTPLGVAPGHPGAGQVYIRSNGTTDDEW
                     TIVTGPEMFGYTSAPLPADQTTTHTVTVVDPRRADPCDITPEEVRIARFATNGRGVAM
                     VYVVLPELEPSDPEICEEGLGRCTRMKAELSLKEDAKPVF"
     assembly_gap    776..788
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <789..2129
                     /locus_tag="PLV68_18405"
     CDS             <789..2129
                     /locus_tag="PLV68_18405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03276.1"
                     /translation="VLGEYVYAEGDGVRIVGRHERTTTTPTAFAPTSLTALSVEAGGH
                     RLEVYADLTQLAVVIDGLLTPLSPNQVRAVGPAISITSIEPTRLTIGVDGALISMYGL
                     LPLTGNHFTNRAQLNTEYTAAAHHRWRGLLGTNDGNQDNDLVGRDGVAITADQAWDHG
                     AAFYGFTESWRLHDPAESPFLLPSDQFGLPNDPRPGDELLEPYRQQAVALLATLETSC
                     AAGPDQAGTLVDQLAIELAIGAATDALIEYACSYTIAGRITVDIPDGDGSFTLPAPGA
                     QVVVEVVGLEACSTTTNSNGDYRCTARPIAGDTIGFPADVWVGVVAPGGDEIATRVKT
                     GTIADRAPMGTTRTSFGNDISLDGHHVRVIDVAMALQRYGQPVTGAVVMVAHHFDDGD
                     DGDALHAVATTVPFVGGSGRLLAAVGPSVTSVDLQLVSEAAGSPMTTIVIAGGG"
BASE COUNT          382 a          784 c          730 g          337 t
ORIGIN      
        1 gcgggcgtgg tcgatctgcc ggtgtacgca ggtgagttca aggtcgtgtg cgccaagacc
       61 ctcggctacg gcctgggcat cgtgccaccg gcggacgggg gcagcgtcga gatccacctg
      121 tcgcgcgacc acccaacgca ggacgtcacc gtgtaccaga ccggccgagt cggcgacgtc
      181 tgggcgctgc acgactacgg cagcaacggc ccgccattca tctcggtcgc cccgatgtcg
      241 ggcgagttcg gccccgaccc gttccccttg accttctcgc tcgactgcat cgacgacgag
      301 acggtcgaca gcacctacta ccagacgatc accgggggcg gtgcccaccc ggcacggatc
      361 gacgtgtact acgactgccg ccccgactac ttcaccgtca cgccactcgg cgtcgcgccg
      421 ggccaccccg gagccggaca ggtctacatc cgctcgaatg gcacgaccga cgacgaatgg
      481 acgatcgtca ccggccccga aatgttcggc tacacaagtg caccgctgcc ggccgaccag
      541 accaccacgc acacggtcac cgtggtcgac ccccgtcggg ccgacccgtg cgacatcacc
      601 cccgaagagg tccgcatcgc acgcttcgcc accaacggcc gcggcgtcgc gatggtgtac
      661 gtggtgctgc ccgagctcga gccatccgat ccggagatct gtgaagaggg actgggcagg
      721 tgcacacgga tgaaggctga gctgtccctg aaggaggatg ccaagcctgt gttctnnnnn
      781 nnnnnnnngt gctcggtgag tacgtgtatg ccgaaggcga cggcgtccgc atcgtcggtc
      841 gccacgagcg caccaccacc acgccgaccg cgttcgcgcc cacctcgctg accgcgctca
      901 gcgtcgaagc tgggggccat cggctcgagg tctacgcgga cctgacccag ttggcggtgg
      961 tgatcgatgg gctgttgacg ccactcagtc ccaaccaggt gcgcgcggtc ggtcccgcga
     1021 tctcgatcac gtcgatcgag ccaacgcggc tgacgatcgg ggtggacggc gcgttgatca
     1081 gcatgtacgg actgttgccg ttgaccggca accatttcac gaacagggcg cagctcaaca
     1141 ccgagtacac cgcggcggcg catcaccgct ggcgcggtct gctcggcacc aacgacggca
     1201 accaagacaa tgatctggtc ggacgcgacg gcgttgcgat caccgcggat caagcttggg
     1261 accacggtgc cgcgttctac ggcttcaccg aatcgtggcg gctccacgac ccggccgagt
     1321 cgcccttcct actgcccagc gatcagttcg gcctgcccaa cgacccgcgg ccaggcgacg
     1381 aactgctgga gccctatcgt cagcaggccg tggccttgct ggccacgctg gagaccagct
     1441 gtgcggccgg accagaccag gccggcacgc tggtcgatca gctcgcgatc gagttggcga
     1501 tcggcgccgc gaccgacgcg ttgatcgagt acgcctgctc gtacaccatc gccgggcgga
     1561 tcacggtcga tattcccgac ggcgatgggt cgttcactct gcccgcgccc ggcgcgcaag
     1621 tcgtggtcga ggtcgtcggc ctggaggcgt gcagcaccac gaccaacagc aacggcgact
     1681 accgctgcac ggcccggccg atcgcgggcg acacgatcgg gttcccggcc gacgtatggg
     1741 tcggcgtcgt cgcaccgggt ggtgacgaga tcgccacccg tgtcaagacc ggcaccatcg
     1801 ccgatcgcgc gccgatgggg acgaccagga catcgttcgg caacgacatc agcctcgatg
     1861 gccaccacgt ccgggtgatc gatgtcgcca tggcactgca gcgctacggc cagccggtca
     1921 ctggcgcggt cgtgatggtt gctcaccact tcgacgacgg tgatgacggc gacgcgttgc
     1981 acgcggttgc cacgacggtc ccgttcgtcg gcggcagcgg tcggttgctc gctgccgtcg
     2041 gccccagcgt cacatcggtc gacctgcaac tcgtcagcga agcagccggg tccccgatga
     2101 ccacgatcgt gatcgccggg ggggggtgac gacggccgag tggaacatct cgtggagccc
     2161 gccgacgatc gacctcaacg gcgcggtgct gatcaacggc gccgcagcgg acggcgcaga
     2221 agtccacgtc atcgcccaac tgcccg
//
LOCUS       DAORNF010000591         2235 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_36362_length_2235_cov_1.790367, whole genome shotgun sequence.
ACCESSION   DAORNF010000591 DAORNF010000000
VERSION     DAORNF010000591.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36362_length_2235_cov_1.790367"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>991)
                     /locus_tag="PLV68_18410"
     CDS             complement(<1..>991)
                     /locus_tag="PLV68_18410"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRE03277.1"
                     /translation="GRKPVLLSGLGLFVAANLFAGLSRSVEMLLMARITAGVAAAMIM
                     PVTLSVITSSFPSEQRARAVGIWSGFAGAGGIIGLFVSSFIVDKLTWPWLFAVPVLFA
                     VVSMVMTLAVVGNSREHADGRFDIAGSVLSAVAIGGIVLGIHEGPEDGWTAPLTLFGL
                     IAGGLALVGFVIWERRIERPLLEIRLFANRGLAAGSLTLLIVFAVMFGIFLVLPQFTQ
                     AVLGYSALKSAAALLPMMAMMMPLSAMAPTIARRIGTRTTLMIGVGLFSTGLMLLAVM
                     VSAERGYWSVLPGLLVLSIGIGLCMSPSTTTITESLPQDKQGVASALNDTVREL"
     assembly_gap    992..1030
                     /estimated_length=39
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1031..1229)
                     /locus_tag="PLV68_18415"
     CDS             complement(<1031..1229)
                     /locus_tag="PLV68_18415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRE03278.1"
                     /translation="MIPPHAHAELVTDPVRQRRILIAMCTALVAVIASVSGLNVAQPH
                     LAADLGASQGQLLWIINGYTMA"
     gene            1411..>1846
                     /locus_tag="PLV68_18420"
     CDS             1411..>1846
                     /locus_tag="PLV68_18420"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRE03279.1"
                     /translation="MDPRVERTRTAVLRAATNLLVEGGPSAVTIDAIVAQSGVAKSTI
                     YRHWDSRDEVLAAVVDAAVPSFPALDPDAPVIDQLAMVVATTVAALNDPEWARVLPAL
                     FLLKHHADGIRAIDDRIEREQDSLVLALLQRGMDEGLLTPGVE"
     assembly_gap    1847..1964
                     /estimated_length=118
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2027..>2235)
                     /locus_tag="PLV68_18425"
     CDS             complement(2027..>2235)
                     /locus_tag="PLV68_18425"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRE03280.1"
                     /translation="PMEVAAQQGYVVVGMQIALDAVRIHGGHGYSTEFDVERYFRDAP
                     LMIVGEGTNEILRGVIANQWMGRQ"
BASE COUNT          402 a          693 c          689 g          294 t
ORIGIN      
        1 caactcacgc acggtgtcgt tcagcgccga cgcgacgccc tgcttgtcct gcggcagcga
       61 ttcggtgatc gtcgtcgtcg acgggctcat gcacaagccg atgccgatcg acagcaccaa
      121 cagaccgggg agcaccgacc aatagccgcg ctcggcagac accatcaccg cgagcagcat
      181 gagtccggtg ctgaacagac cgacgccgat catcaaggtg gtccgcgtgc cgatccgacg
      241 ggcgatcgtg ggagccatcg ccgacagcgg catcatcatc gccatcatcg gcagcagcgc
      301 cgcggccgac ttgagcgctg agtagccgag aaccgcttgg gtgaactggg gcagcaccag
      361 gaagatgccg aacatcaccg cgaagacgat cagcagggtc aacgacccag cggccagacc
      421 gcggttggcg aacaggcgga tttccagcag cgggcgctcg atgcggcgct cccagatcac
      481 gaagccgacc aacgccagac caccggcaat cagaccgaac aaggtcaacg gcgcggtcca
      541 gccgtcctct gggccttcgt ggatgccgag cacgatgccg ccgatcgcga ccgccgacag
      601 caccgatcca gcgatgtcga agcgaccatc ggcatgctca cgcgagttgc cgaccaccgc
      661 cagcgtcatc accatcgaga ccacagcaaa caacaccggc acggcgaaca accacggcca
      721 ggtgagcttg tcgacgatga acgacgacac gaacaggccg atgatgccac cagcaccggc
      781 aaagccggac cagatgccca cggcccgcgc ccgctgctcg gacgggaagc tcgacgtgat
      841 gaccgacagc gtgacgggca tgatcatcgc cgccgcgaca ccggcggtga tgcgcgccat
      901 cagcagcatc tcgaccgaac gagagagccc ggcgaacaga ttggccgcca cgaacaggcc
      961 gagaccgctc agcagcaccg gcttgcggcc gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1021 nnnnnnnnnn gcgccatcgt gtagccgttg atgatccaga gcaactggcc ctgcgacgcg
     1081 ccgaggtcgg cggcgagatg gggctgggcg acgttgaggc cgctgaccga ggcgatcacg
     1141 gcgaccagcg cggtgcacat cgcgatcaag atccgtcgct ggcgaaccgg atcggtcacc
     1201 agttcggcat gggcgtgggg gggaatcacg atgtctcctc tcgagacagc gccgtcaagt
     1261 gcttggggca agcgtgtggg gtcagcgcgt tggggggtgg ggtcatttca gtacgagacg
     1321 gtatcgtact gaaacatgaa cgcaccacca cccaccacga cgacgttgcg ttcgtcacac
     1381 ctctgctcgg ggccagtacc gtcgcggccg atggatcccc gagtcgagcg gacccgcacc
     1441 gccgtgttgc gggccgccac gaacctgctg gtcgaaggcg ggccgagcgc ggtcaccatc
     1501 gacgcgatcg tcgcccagtc gggtgtcgcc aagtcgacca tctatcgcca ttgggacagt
     1561 cgcgacgagg tgctggccgc ggtggtcgac gcggcggtgc cgagctttcc cgcgctcgac
     1621 cccgacgcac ccgtgatcga tcagctggcc atggtggtcg cgaccaccgt cgctgcgctg
     1681 aacgaccccg agtgggcccg cgtgctgccc gcgctgttcc tgttgaagca tcacgccgac
     1741 ggcatccgtg cgatcgacga tcgcatcgaa cgcgagcagg attcgctggt gctcgcgctg
     1801 ctgcagcgag gcatggacga aggcctgttg acaccgggcg tcgaagnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngtgatg cggtcttgac
     1981 cgggatgcag tcgtgaccgg gatgcggcgt cgacggggat gctgcgctac tggcgaccca
     2041 tccactggtt ggcgatgacg ccgcgcaaga tctcgttggt gccctcgccc acgatcatca
     2101 gcggggcgtc gcggaagtag cgctcgacgt cgaactcggt ggagtagccg tgcccgccgt
     2161 ggatccgcac cgcgtcgagc gcgatctgca tgccgacgac gacatagcct tgctgggcgg
     2221 cgacttccat cggac
//
LOCUS       DAORNF010000592         2235 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_36365_length_2235_cov_1.586239, whole genome shotgun sequence.
ACCESSION   DAORNF010000592 DAORNF010000000
VERSION     DAORNF010000592.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36365_length_2235_cov_1.586239"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /locus_tag="PLV68_18430"
     CDS             complement(<1..370)
                     /locus_tag="PLV68_18430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03281.1"
                     /translation="MVTVPLLHRGAQAGVEQTVWRRRHEPSGAGGRGRHEGTGDVDDH
                     TLGAALGEHRQRTVVAQRDQRAVGPNLAGGVVEVRRRRPLDTGRPHGEEAVAVGVVDG
                     DRDSDRNRVGGHVAAVRGDDF"
     gene            387..>737
                     /locus_tag="PLV68_18435"
     CDS             387..>737
                     /locus_tag="PLV68_18435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HRE03282.1"
                     /translation="MPADATAVAVTVTVTEASRAGFVTAWAAGTTQPWASTVNADGPG
                     QTRAAGAVVPVSDSGITLFASASTHVVVDLAGYFTGESADEDTAGFFQPIVPERVLDT
                     RESTRLAPGTSRDIA"
     assembly_gap    738..750
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <751..>1127
                     /locus_tag="PLV68_18440"
     CDS             <751..>1127
                     /locus_tag="PLV68_18440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03283.1"
                     /translation="VAANVTIVQPSTPGWMVLYPTGPTVPGTSSVNATSAGEIAASLA
                     LVASPDGVSVYAHPETDAVVDVTGVFTPTRSSAVPDGTAVVPPTTTTTAPPGPAAEYS
                     FAYTSRNWTPTAYDTCPASLHES"
     assembly_gap    1128..1196
                     /estimated_length=69
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1197..>2235
                     /locus_tag="PLV68_18445"
     CDS             <1197..>2235
                     /locus_tag="PLV68_18445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03284.1"
                     /translation="EMCNFGHEHGDDPATSDIYDWAMEQLSPSTGDSATGIPFGLASE
                     ALITYSNNGVTQANRHEDNVGHKVFVKNDLQLISQSPREWVRLPDGTPVKCDYLIKTH
                     QGSHSADATTNNAHELFYAVRCNDGTELAVTTLSRFGDANQYHRSCAPGQVVTTSGSN
                     LPDGDGGKRLIPDRGCLEQYVLVPPTGFSDLWSFYEVWQSANEITTTGGDVLATYDPW
                     FGVRNPSRYAWSGKDDGVDGLGATVDAAWETDPNDGGKVTADPWLAICTLPPIPKASP
                     ESPFDGAQRDLYLRTTIIANDGGASVWYTDPYGANASTTPFPGSIRQVISATADPGYP
                     TLERQNFGLGH"
BASE COUNT          350 a          816 c          694 g          293 t
ORIGIN      
        1 cgaagtcgtc gccgcggacc gctgcaacgt gcccgccgac gcggttgcgg tcgctgtcac
       61 ggtcaccgtc gacaaccccg acggcgacgg cttcctcacc gtgtggccga ccggtgtcga
      121 gcggccgacg acgtcgaacc tcaactacac caccggccag attcgggcca acggcgcgct
      181 gatcgcgctg ggcgacgacg gtgcgctgtc ggtgttcacc cagcgccgcg ccgagggtgt
      241 gatcgtcgac gtcaccggtg ccttcgtgcc tgccgcggcc gccagcaccg gacggttcgt
      301 ggcgacgacg ccagaccgtc tgctcgacac ccgcctgagc accccggtgc aacagtggca
      361 cggtcaccat cccgctaccg acgggcgtgc ccgccgacgc gaccgctgtc gccgtcaccg
      421 tgacggtcac cgaggcctcg cgcgccgggt tcgtcaccgc ctgggcggcc ggcaccaccc
      481 agccgtgggc atcgacggtc aacgccgacg gccctggcca gacccgtgct gctggcgcgg
      541 tggtaccggt gagcgacagc ggcatcacgc tgttcgcctc ggcctcgacc cacgtcgtgg
      601 tcgacctggc cgggtatttc accggcgagt cggccgacga ggacaccgcc ggcttcttcc
      661 agccgatcgt gcctgagcgg gtgctcgaca cccgcgaatc gactcgcctg gccccgggca
      721 cgtcgcgaga catcgcgnnn nnnnnnnnnn ggtggcggcc aacgtcacga tcgtccaacc
      781 gtcgactccc ggctggatgg tgctctaccc gaccggcccg acggtgcccg gtacgtccag
      841 cgtcaacgcg acatcggcag gcgagatcgc ggcgagcctc gcgctggttg cgtcgcccga
      901 cggcgtgtcg gtctacgccc acccggagac cgacgccgtg gtcgatgtca ctggcgtgtt
      961 cacccccacc cgctcgtccg ccgtgccgga cggcaccgcg gtcgtcccgc cgaccacgac
     1021 cacgaccgcg ccgcccggcc cggcggcgga gtactcgttc gcctacacgt cgcgcaactg
     1081 gaccccgacc gcgtacgaca cctgcccggc ctcgctgcac gagtcgtnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngcga
     1201 gatgtgcaac ttcggccacg agcacggcga tgacccggcg acgtcggaca tctacgactg
     1261 ggcgatggag caactctcgc cgagcacggg agattccgcc accggcatcc cattcggcct
     1321 cgcgtccgag gcgctgatca cgtactcgaa caacggcgtg acacaggcca accggcacga
     1381 ggacaacgtc ggccacaagg tgttcgtgaa gaacgacctc caactcatca gccagtcacc
     1441 ccgtgagtgg gtgcgcctgc ccgacggcac gcctgtcaag tgcgactacc tcatcaagac
     1501 ccaccaaggc agccactcgg ccgacgccac caccaacaac gcacacgagc tgttctacgc
     1561 ggtgcgctgc aacgacggca ccgagttggc ggtcaccacg ctgagccgct tcggcgacgc
     1621 caaccagtac caccgcagct gcgcccccgg ccaggtcgtc accaccagcg gcagcaacct
     1681 gcccgacggc gacggcggca agcggttgat ccccgaccgc ggctgcctgg agcagtacgt
     1741 gttggtgccc ccgaccgggt tcagcgatct ctggtcgttc tacgaggtct ggcagtcggc
     1801 caacgagatc accacgaccg ggggcgatgt gctcgccacg tacgacccgt ggttcggcgt
     1861 gcgcaacccc agccgctatg cgtggagcgg caaggacgac ggtgtcgacg gcctcggcgc
     1921 cacggtcgac gcggcatggg agaccgaccc caacgacggt ggcaaggtca cggccgaccc
     1981 gtggttggcg atctgcacgc tgccgccgat tcccaaggcg tcgccggagt cacccttcga
     2041 cggggcgcag cgcgacctgt acctgcgcac gacgatcatc gccaacgacg gtggggcgag
     2101 cgtgtggtac accgacccct acggggccaa cgcgagcacg acgccgttcc ccggctcgat
     2161 ccgccaggtg atcagcgcca cggccgaccc cggctacccc accctggagc ggcagaactt
     2221 cggcctcggt catga
//
LOCUS       DAORNF010000593         2233 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_36414_length_2233_cov_1.525253, whole genome shotgun sequence.
ACCESSION   DAORNF010000593 DAORNF010000000
VERSION     DAORNF010000593.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36414_length_2233_cov_1.525253"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="PLV68_18450"
     CDS             complement(<1..273)
                     /locus_tag="PLV68_18450"
                     /inference="COORDINATES: protein motif:HMM:NF019880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03285.1"
                     /translation="MDDAHRASGAVDRGDQSGAADQRPSLRVTQRRLAVQRDARGRAI
                     SLGGRSLDPNARAKYLNGPETELFDKGRNLFNQSPAREAAGKGQRLI"
     gene            259..>471
                     /locus_tag="PLV68_18455"
     CDS             259..>471
                     /locus_tag="PLV68_18455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03286.1"
                     /translation="MRIIHAHPDPPTMRSGIADWDASFAAMSDAGLAQGPPTLIVADL
                     NAARWHPSWRRLVRRGWRDAHEIAGRG"
     assembly_gap    472..531
                     /estimated_length=60
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            652..1299
                     /locus_tag="PLV68_18460"
     CDS             652..1299
                     /locus_tag="PLV68_18460"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HRE03287.1"
                     /translation="MELGDARIVTVSAAYGAGGSVVAPQLAERLGLPFFNRLIPDDRH
                     NSGDAIRERLTAEERDQAPPGRIVASLSALSSTLGLPVTGIDDMDPRIDLRRRVETSV
                     TRIATDGGGVILGRAGAVVLAGTRNAFHVRLYGPAEACKVQGAEIEAISLAEAAQHQA
                     DSDRAWTRFVNRLFDRNPQDASLYHLLIDSTAIPLTTTIELVSQAAHAFWQHSAQ"
     gene            1361..2206
                     /gene="panB"
                     /locus_tag="PLV68_18465"
     CDS             1361..2206
                     /gene="panB"
                     /locus_tag="PLV68_18465"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HRE03288.1"
                     /translation="MTDQPALTGGTADPASKRVRIRHFARAKEQGIKITGLTSYDYLS
                     AQIFDAAGIDFLLVGDSAGNTVFGYDTTLPVTVDELIPLTRAVVRAAKRALVIGDMPF
                     GSYENGPKEALHTAIRFMKESGCHAVKLEGGVRSADQIERIVRAGIPLMGHIGFTPQS
                     EHGLGGHVIQGRGAGAAQLLADARAVEQAGAFAVVLEMVPSAVAAQVTAELSIPTISV
                     GAGPHCDGQLMVWSDWAGFTTGRIPKFVKRYADIASVLHQAAVQYKADVDDGAYPAAE
                     HEYSD"
BASE COUNT          357 a          793 c          704 g          319 t
ORIGIN      
        1 gatcagccgc tggcccttgc cggccgcctc gcgcgcgggg gactggttga agaggttgcg
       61 gcccttgtca aacagctcgg tttccggccc gttcaggtat ttcgcccgcg cattggggtc
      121 cagcgaccgg ccgcccaggc tgatggcgcg gccgcgggca tcgcgctgta cagcaagacg
      181 ccgctgagtg acgcgcagcg acggccgctg gtcagccgcc ccggattggt cgccacggtc
      241 gactgccccg ctggcccggt gcgcatcatc cacgcccatc ccgatccacc gacgatgcgc
      301 agcggcatcg ccgactggga cgcctcgttc gcagcgatgt cggacgccgg tctggcccaa
      361 ggaccgccca ccctgatcgt ggccgacctc aacgccgctc gatggcatcc gagttggcga
      421 cggctcgtgc gacggggttg gcgcgacgcg cacgagatcg ccggccgtgg tnnnnnnnnn
      481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntggtgcgca
      541 acgatctcga cgtcgtcgcg gtcaccgact tcgacgtccc gggcagtgat catcggggct
      601 tcgtcgtgct cgtgagcccg gcatcgacat ccacaaacta gggtccgcaa catggaactc
      661 ggagacgcac gaatcgtgac ggtgtcggcc gcctacggcg cgggcggcag tgtcgtcgca
      721 ccacagcttg ccgagcggct gggtctgccg ttcttcaatc gcctcatccc tgacgatcgt
      781 cacaactcgg gcgacgcgat ccgcgagcga ctcaccgccg aagagcgcga ccaggcaccc
      841 cccggacgca tcgtcgccag cctcagcgcg ctcagcagca cgctcggatt gccggtcacg
      901 ggcatcgacg acatggaccc gcgcatcgac ctgcgccggc gcgtcgagac cagcgtgacg
      961 cgcatcgcca ccgacggtgg tggcgtcatc ctcggccgcg ccggagccgt cgtgctggcc
     1021 gggacccgca acgcgttcca cgtccgcctc tacgggccgg ccgaagcgtg caaggtccag
     1081 ggcgcggaga tcgaggcgat ctcgctggcc gaagcggccc agcaccaagc cgactccgac
     1141 cgcgcctgga cccgcttcgt caaccgtctg ttcgaccgca atccgcagga cgcgtcgctc
     1201 tatcacctgc tgatcgactc caccgcgatc cccctcacga ccacgatcga gctcgtcagc
     1261 caggccgcac acgccttctg gcagcactcg gcgcagtgat gctcacccac ggaccgggcg
     1321 tcgtctggca cagatcgacg cgccaccgta gggttgggtc gtgaccgacc agccagcact
     1381 caccggtggt accgcagacc ccgccagcaa gcgcgtccgc atccgccact tcgctcgggc
     1441 caaagagcag ggcatcaaga tcaccgggtt gacgagctac gactacttgt cggcacagat
     1501 cttcgacgcc gcgggcatcg acttcttgct cgtcggcgac tcggcgggca acacggtgtt
     1561 cggctacgac accacgctgc ccgtcaccgt cgacgagctg atcccgttga cccgggccgt
     1621 cgtacgggcg gccaagcggg ccttggtcat cggcgacatg ccgttcggct cgtacgagaa
     1681 cgggccgaag gaagcgctcc acaccgcgat ccgcttcatg aaggagtccg gctgccacgc
     1741 cgtgaagctc gaaggaggcg tacgcagcgc cgaccagatc gaacgcatcg tgcgcgccgg
     1801 catcccgctg atgggccaca tcggcttcac cccgcagagc gagcacggcc tcggtggcca
     1861 cgtcatccaa ggacgcggcg caggcgcggc ccaactgctc gccgacgccc gtgccgtcga
     1921 gcaggccggg gcgttcgcgg tggtgctgga gatggtgcca tcggccgttg ccgcccaggt
     1981 caccgccgag ctgtcgatcc ccacgatcag cgtcggtgcc ggaccgcatt gcgacggcca
     2041 gctgatggtg tggagtgact gggccgggtt caccaccggc cggatcccca agttcgtcaa
     2101 gcgatacgcc gacatcgcct cggtgctcca ccaagccgcg gtgcagtaca aggccgacgt
     2161 cgacgacggc gcctatccag cagccgagca cgagtacagc gactgatccg cgaaccgccc
     2221 accgaggtcc gta
//
LOCUS       DAORNF010000594         2228 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_36517_length_2228_cov_1.551772, whole genome shotgun sequence.
ACCESSION   DAORNF010000594 DAORNF010000000
VERSION     DAORNF010000594.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36517_length_2228_cov_1.551772"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>216
                     /locus_tag="PLV68_18470"
     CDS             <1..>216
                     /locus_tag="PLV68_18470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HRE03289.1"
                     /translation="GQGSRLMGFGHRVYKNYDPRATIIKKTAYEVFEVTGKNPLLDIA
                     LKLEEVALSDDYFISRKLYPNVDFYSGL"
     assembly_gap    217..462
                     /estimated_length=246
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<463..796)
                     /locus_tag="PLV68_18475"
     CDS             complement(<463..796)
                     /locus_tag="PLV68_18475"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HRE03290.1"
                     /translation="MIGGDYVDRNLRAVASQGTIVQVGLMGGGSTSVNVGLLLARRAH
                     WIGTTLRPRPIEQKVAIVQRFIREVLPLFDTGALRPVIDTRFSLDDIQAAHARMGTNA
                     NTGKILIDV"
     gene            complement(793..1413)
                     /locus_tag="PLV68_18480"
     CDS             complement(793..1413)
                     /locus_tag="PLV68_18480"
                     /inference="COORDINATES: protein motif:HMM:NF019845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HRE03291.1"
                     /translation="MRAVVLTDHNGPASLVVRDAPEPVCGPDDVLVEITATALNRADL
                     LQTRGFYPDPFPGPTEIPGMECSGIVATVGSRVRDVAVGDAVMAITSGGAYAERIAIH
                     HRQVMQVPAVVGVADAAAIPEVFITAWDALVVQGGLTSGRWALVHAGASGVGTAAIQI
                     VKALGGRVAVTCSAGKVEACRTLGADLVIDYASTDFVDAVSTPCST"
     gene            complement(1452..>1954)
                     /locus_tag="PLV68_18485"
     CDS             complement(1452..>1954)
                     /locus_tag="PLV68_18485"
                     /inference="COORDINATES: protein motif:HMM:NF012407.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HRE03292.1"
                     /translation="DAAGLTYEHRLIDDMVAAAMKWEGGYVWACKNYDGDVQSDTVAQ
                     GFGSLGLMTSVLMTPDGKTVEAEAAHGTVTRHYREHQKGNKTSTNPIASIYAWTQGLT
                     YRGKMDDTPDVVAFAQALESVCVEAVEAGEMTKDLALLISKDAPWLTTEDFLDALDRR
                     LQAKMG"
     assembly_gap    1955..1964
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1965..>2228)
                     /locus_tag="PLV68_18490"
     CDS             complement(<1965..>2228)
                     /locus_tag="PLV68_18490"
                     /inference="COORDINATES: protein motif:HMM:NF012407.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HRE03293.1"
                     /translation="KVPGSGTVTLTYTPADGSAPVEMEVAQFGPDGGVTMGMYNYNDS
                     IRDFARASLRYGLNRGYPVYLSTKNTILKAYDGQFKDIFQEVY"
BASE COUNT          313 a          654 c          662 g          343 t
ORIGIN      
        1 ggtcagggca gccgcctgat gggcttcggt caccgggtct acaagaacta cgacccgcgg
       61 gccacgatca tcaagaagac ggcctacgag gtgttcgagg tcaccggcaa gaatccgctg
      121 ctcgacatcg cgctcaagct cgaagaggtc gccctcagcg acgattactt catcagccgt
      181 aagctgtatc ccaacgtcga cttctactcg ggcctgnnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngcacgtcg atcaggatct
      481 tgccggtgtt ggcattggtc cccatgcggg cgtgtgcggc ttggatgtcg tcgaggctga
      541 agcgggtgtc gatcacggga cgcagcgcgc cggtgtcgaa caacggcagc acctcgcgga
      601 tgaagcgttg gacgatcgcg accttctgct cgatcggacg cggccgcagg gtcgtgccga
      661 tccagtgggc acgcctggcc agcaacagac cgacgttgac cgacgtcgag ccgccgccca
      721 tcaaaccaac ctgcacgatg gtgccctggc tggcgacggc gcgcaggttg cgatcgacgt
      781 agtcaccgcc gatcacgtcg agcacggtgt cgataccgcg tcgacgaagt cggtgctggc
      841 gtagtcgatc accaggtcgg ctccgagggt gcgacacgct tcgaccttgc ccgcgctgca
      901 cgtcaccgcg acccgcccac cgagcgcctt gacgatctgg atcgcggccg tgcccacacc
      961 cgacgcgccg gcgtggacca acgcccagcg gccgctggtc agcccaccct gcacgaccag
     1021 ggcatcccac gcggtgatga acacctcggg gatcgcggct gcgtcggcca cgccgaccac
     1081 cgccggcact tgcatcacct ggcggtggtg gatcgcgatc ctttcggcgt aagcaccacc
     1141 cgacgtgatc gccatcactg catcgccgac ggcgacgtcg cgcacccgcg agcccactgt
     1201 cgcgacgatg ccgctgcact ccatgccggg aatctcggtc gggccgggga acgggtccgg
     1261 gtagaacccc cgcgtctgca acaggtcggc ccggttgagg gcggtggcgg tgatctccac
     1321 caacacgtcg tcagggccgc acaccggctc gggtgcgtcc ctgacgacga ggctggcagg
     1381 tccgttgtga tcggtcagaa cgacggcgcg catccgttca ttctgacgcg gcacgcaacg
     1441 ccgcgtgccg atcagcccat cttggcctgg aggcgccggt cgagcgcgtc gaggaagtcc
     1501 tcggtggtca gccacggggc gtccttgctg atcagcaggg cgagatcctt ggtcatctcg
     1561 cctgcctcga ccgcctcgac gcagactgac tcgagcgcct gagcgaaggc caccacgtcg
     1621 ggggtgtcgt ccatcttgcc gcggtaggtc aggccctggg tccaggcgta gatcgacgcg
     1681 atcgggttgg tgctggtctt gttgcccttc tggtgctcgc ggtagtggcg cgtcaccgtg
     1741 ccgtgggcag cttcggcttc gaccgtcttg ccatcggggg tcatcagcac cgaggtcatc
     1801 aggcccagcg agccgaaacc ctgggcgacc gtgtcggact ggacgtcgcc gtcgtagttc
     1861 ttgcaggccc acacgtagcc gccctcccac ttcatcgccg cggcgaccat gtcgtcgatc
     1921 aggcggtgct cgtaggtgag cccggccgcg tccannnnnn nnnncgtaga cctcttggaa
     1981 gatgtccttg aactgtccgt cgtaggcctt gaggatcgtg ttcttggtgc tgaggtacac
     2041 gggatagccg cggttgaggc cgtaacgcag cgaggcccgg gcgaagtcgc ggatgctgtc
     2101 gttgtagttg tacatgccca tcgtgacgcc gccgtcgggg ccgaactgag cgacttccat
     2161 ctcgaccggc gcgctgccgt cggcgggggt gtaggtgagc gtgaccgtgc cggagccggg
     2221 gaccttgt
//
LOCUS       DAORNF010000595         2226 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_36550_length_2226_cov_2.076462, whole genome shotgun sequence.
ACCESSION   DAORNF010000595 DAORNF010000000
VERSION     DAORNF010000595.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36550_length_2226_cov_2.076462"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="PLV68_18495"
     CDS             <1..346
                     /locus_tag="PLV68_18495"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HRE03294.1"
                     /translation="VWAVNADNVVVRSYLVAGSQYANEQPGTHKVYSKSEKSTAWNGQ
                     AWLPLMVRWLKTERGAIGFHAIPLHVKDNSPYMTDAEMGQRLSGGCQRQTDLDAKFMW
                     DFAEIGTKVVVI"
     gene            complement(398..1252)
                     /locus_tag="PLV68_18500"
     CDS             complement(398..1252)
                     /locus_tag="PLV68_18500"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HRE03295.1"
                     /translation="MLAAIAGQTERIRLGSAVTVLSSDDPVRVYQRFATLDAISHGRA
                     EVILGRGSFTESFPLFGYDLQDYERLFDEKLDLFAALRTEGPIEWSGTTRPALHGQRV
                     YPTTESGVLTTWVGVGGSPESVVRTARYGLDLMLAIIGGDPRRFARYVELFHRALREL
                     GQPMRRIGVHSPGFVAATDDEARELLWPHFKEHRDRIGRDRGWGPVTRAAFDHEAGPN
                     GSLYVGSPETVALKIASTVEALGADRFDLKYSNGSLPHEHLMRSIELYGTEVIPRVRE
                     LLGERVAA"
     gene            1273..1515
                     /locus_tag="PLV68_18505"
     CDS             1273..1515
                     /locus_tag="PLV68_18505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03296.1"
                     /translation="MVFAHTDEVDTDAVSQHGLLDHLADHVWVGDGTATGIDGDVTEG
                     VEAQFDCAGFERGEMRCCDLVHPRSFLSQGHTIGPK"
     assembly_gap    1621..1655
                     /estimated_length=35
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1660..>2226
                     /locus_tag="PLV68_18510"
     CDS             1660..>2226
                     /locus_tag="PLV68_18510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRE03297.1"
                     /translation="MSDSIGMYLNEIGRVALLTAEEERELSKAIEEGRDAAERLANGE
                     KGALLKAQVRTAAEAKDRFIRANLRLVVSIARRYPLPQGMDLGDLIQEGNLGLEHAVD
                     KFDWRRGFKFSTYATFWIRQAIGRALDQKASLIRIPGDRSASLRAALRQASGDGETLD
                     LNNAELHRLTTPVSLDKTIGDDGDATLGD"
BASE COUNT          398 a          733 c          718 g          342 t
ORIGIN      
        1 cgtgtgggcc gtcaacgccg acaacgtggt ggtgcgctca tacctggtgg ccggtagcca
       61 gtacgccaac gagcagcccg gcacgcacaa ggtgtacagc aagtccgaga agtccacggc
      121 atggaatggc caggcctggc tgcccctgat ggtgcgctgg ttgaagaccg agcggggcgc
      181 gatcggcttc cacgccattc ccttgcacgt caaggacaac tcgccgtaca tgaccgacgc
      241 cgagatgggc cagcgcttgt ccggcggctg ccagcgccag accgacctcg acgccaagtt
      301 catgtgggac ttcgccgaga tcggtaccaa ggtcgtcgtc atctgaccgg acgtcagcgc
      361 cgcttggtca gtctccgatt ggtcagtctc cgattggtca ggcggccacc cgctcgccga
      421 ggagttcacg cacccgcggg atcacctcgg tgccgtagag ctcgatcgag cgcatcaggt
      481 gctcatgtgg caacgacccg ttcgagtact tgaggtcgaa ccggtcggcg ccgagcgcct
      541 cgacggtcga cgcgatcttg agcgcaaccg tttcgggcga gcccacgtac aacgaaccgt
      601 tagggccggc ctcgtggtcg aaggcagccc gcgtcaccgg accccatccg cgatcgcggc
      661 cgatgcggtc gcgatgctcc ttgaagtgcg gccacaacag ctcccgcgcc tcgtcgtcgg
      721 tcgcggcgac gaagccgggc gagtgcaccc cgatccggcg catgggctgg cccagctcgc
      781 gcaacgcgcg gtggaacagc tcgacgtagc gagcgaaccg gcgcggatca ccgccgatga
      841 tcgccagcat caggtcgagc ccgtagcggg cggtgcgcac gaccgactcg ggactgccgc
      901 cgacgccgac ccacgtggtc agcacaccag actcggtggt cgggtagacg cgctggccgt
      961 gcaaggccgg gcgggtcgtg ccgctccatt cgatcgggcc ctcggtacgc aacgcggcga
     1021 acaggtcgag cttttcgtcg aacaggcgct cgtagtcctg caggtcgtag ccgaacaggg
     1081 ggaacgactc ggtgaacgag ccccggccga gaatcacctc ggcccggccg tgcgagatcg
     1141 cgtcgagcgt ggcgaagcgt tggtagacgc ggaccgggtc gtcggaactg agcacggtga
     1201 ccgccgagcc gagccggatc cgctcggtct gcccggcgat cgcggcgagc acgacgtcgg
     1261 gaagtcatcg cgatggtgtt cgcccacacc gatgaagtcg acaccgacgc ggtcagccag
     1321 cacggcttgc tcgatcacct ggcggatcac gtctgggtgg gcgacgggac ggccaccggc
     1381 atcgacggtg acgtcaccga aggtgtcgag gcccagttcg actgtgccgg gttcgaacgt
     1441 ggcgagatgc gatgttgtga cctggttcat cccaggagct tcctttcaca gggccacacg
     1501 atcgggccaa agtagttgat acatcatcta acatcgatgc agtccacggt attcctcgac
     1561 ctgaacttcc cgaccccatc tcgactggcg tgacgcgcgg cacatgatgg tcgggaagca
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncgtca tgagcgattc catcggtatg
     1681 tacttgaacg aaatcggccg agtggccctg ttgaccgccg aagaggagcg cgagctctcc
     1741 aaggcgatcg aagagggtcg tgacgccgca gagcgcctcg ccaatggcga gaagggtgcg
     1801 ttgttgaagg cacaggtccg cacggccgca gaggccaagg accgtttcat ccgcgccaac
     1861 ctccggctgg tggtcagcat cgcccgccgt tatcccctcc cccagggcat ggacctgggt
     1921 gacctgatcc aagagggcaa cctcggtctc gaacacgccg tcgacaagtt cgactggcgc
     1981 cgcggcttca agttcagcac ctatgccacg ttctggatcc gtcaggccat cggccgcgcc
     2041 ctcgaccaga aggccagcct gatccgtatc cccggtgacc gctcggccag ccttcgcgct
     2101 gcgctgcgcc aggccagcgg cgacggcgag acgctcgacc tcaacaacgc cgaactgcat
     2161 cgcctgacca cgccggtctc gctcgacaag accatcggtg acgacggcga cgccaccctc
     2221 ggcgac
//
LOCUS       DAORNF010000596         2226 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_36552_length_2226_cov_1.830032, whole genome shotgun sequence.
ACCESSION   DAORNF010000596 DAORNF010000000
VERSION     DAORNF010000596.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36552_length_2226_cov_1.830032"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1144)
                     /locus_tag="PLV68_18515"
     CDS             complement(<1..>1144)
                     /locus_tag="PLV68_18515"
                     /inference="COORDINATES: protein motif:HMM:NF023326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3418 domain-containing protein"
                     /protein_id="HRE03298.1"
                     /translation="PDRVHQAVLSGLLSHLGLRDGESREFRGARQSKFMISPGSVTAK
                     RPPQWVMAAELVETNRLWARTVAKVEPEWAERLAPHLLKRSYGEPRWDERSGRAVTSE
                     RVTLYGLPIVASRTIGVDRLDPALAREMFIRHALVAGEWNHRSRPIEANRAFVADARQ
                     LGARVRRWDLVGDDAVFAFYDRRIGPEVVSARHFDRWWKATRQTQPQLLRMTLADLSG
                     DGALTAADFPDTWQHGEIALPVTYRFEPDAPGDGVTVHIPIAVLNQIDATGFDWQVPG
                     FREDLVDALVRSLPKDVRRELNPLNTSVAAVWAQVRLASPATGTLADTVATAVAAVSG
                     VRTPADAFDARNVDGHHRITFSVEEQDGTVVATGKDLDAVRRLVGGR"
     assembly_gap    1145..1268
                     /estimated_length=124
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1269..>1946)
                     /locus_tag="PLV68_18520"
     CDS             complement(<1269..>1946)
                     /locus_tag="PLV68_18520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HRE03299.1"
                     /translation="ISRYSARLKVQRLPIEPVSQASANQRAGRCGRVAPGVCIRLYSE
                     ADFQARPAFTEPEILRTNLASVILQMTALGLGDVAAFPFIDPPDSRSVRDGVALLEEL
                     GALELDRPADDRRLTALGRRLTRLPVDPRLGRMVLEAERHGCVREVLVIAAALSIQDP
                     RERPSDKQQAAAEMHGRFTVEGSDFMTLVALWDYLRERQHELSSSQFRKLCRNEFLHY
                     LRVREWQ"
     assembly_gap    1947..1973
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1974..>2226)
                     /locus_tag="PLV68_18525"
     CDS             complement(<1974..>2226)
                     /locus_tag="PLV68_18525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HRE03300.1"
                     /translation="VDELLDDTDGDVLVFLSGEREIHDVADALRRDERRQRAIGHGTF
                     DVLPLYARLSAAEQHRIFEPHRGRRVVLSTNVAETSLTVP"
BASE COUNT          323 a          741 c          713 g          298 t
ORIGIN      
        1 cccgcccgcc gaccagacgg cgcacggcgt cgaggtcctt gcccgtcgcc accacggtgc
       61 cgtcctgctc ttcgaccgag aacgtgatcc ggtggtggcc gtcgacgttg cgggcatcga
      121 acgcatcggc tggggtgcgt accccgctga ccgcagcgac cgcggtcgcg acggtgtcgg
      181 cgagtgtgcc ggtcgcggga gaggcaagcc gtacctgcgc ccacaccgcg gccaccgagg
      241 tattgagcgg gttgagctcg cggcgcacgt ccttgggcag cgaccgcacc agcgcgtcga
      301 ccaggtcctc gcgaaagccc ggcacctgcc aatcgaatcc cgtcgcgtcg atctggttca
      361 acaccgcgat cgggatgtgc acggtgacgc cgtcgcccgg tgcgtccggt tcgaagcggt
      421 acgtcacggg caaggcgatc tcgccgtgct gccacgtgtc gggaaagtcg gccgccgtga
      481 gggcaccgtc gcccgacagg tcggccaggg tcatccgcag caactgcggc tgggtctggc
      541 gggtcgcctt ccaccaccgg tcgaagtgtc gggccgacac gacctcaggc ccgatccgac
      601 ggtcgtagaa cgcaaacacg gcgtcgtcac ccaccaggtc ccagcgccgc acccgcgcgc
      661 cgagttgacg agcatcggcg acgaacgcgc gattggcctc gatcgggcgg ctgcggtggt
      721 tccactcccc ggcgaccaac gcgtggcgga tgaacatctc ccgtgccagc gcagggtcga
      781 gtcggtcgac accgatcgtt cgcgatgcaa cgatcggtag gccgtacagc gtgacgcgct
      841 cactggtcac cgcccgtccg ctgcgctcgt cccaacgcgg ctcgccgtag ctgcgcttga
      901 gcagatgcgg ggcaagccgt tcggcccact cgggctcgac cttggccacg gtgcgcgccc
      961 acagccggtt ggtctccacc agttccgcgg ccatcaccca ctgcggtggc cgcttcgcgg
     1021 tgaccgatcc cggcgagatc atgaacttcg actgccgtgc cccccggaac tcgcggctct
     1081 cgccatcgcg caggccaagg tgcgagagca ggccgctgag caccgcttgg tgaacgcggt
     1141 cgggnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnncc tgccactcgc gcacccgcag gtagtgcaag aactcgttgc ggcacagctt
     1321 gcggaactgg ctggaggaca gctcgtgctg gcgctcgcgc aggtagtccc acagggccac
     1381 cagcgtcatg aagtccgatc cctccaccgt gaagcggccg tgcatctcgg cggccgcctg
     1441 ctgcttgtca ctcgggcgct cgcgcgggtc ttggatcgac agcgcggcgg cgatcaccag
     1501 cacctcgcgg acgcagccat gacgctcggc ctccagcacc atccgcccga gccgtggatc
     1561 gaccggcaga cgggtgaggc gccggccgag cgcggtcaga cgccggtcgt cggcaggccg
     1621 gtcgagctcg agcgcgccga gctcttccaa cagcgccacg ccgtcgcgca ccgaacgcga
     1681 gtcgggcgga tcgatgaacg ggaacgccgc cacatcgccc agccccaacg cggtcatctg
     1741 caggatgacc gaggccaggt tggtgcgcaa gatctccggc tcggtgaacg ccgggcgagc
     1801 ctggaagtcg gcctcgctgt agaggcgaat gcacaccccg ggagcgaccc ggccgcagcg
     1861 cccggcacgc tggttggccg acgcctgcga cacgggctcg atcggcaggc gttgcacctt
     1921 gagccgcgcg ctgtagcggg agatgcnnnn nnnnnnnnnn nnnnnnnnnn nnnccggcac
     1981 ggtcagcgac gtctcggcga cgttggtcga caacacgacg cggcggccgc ggtggggctc
     2041 gaagatacgg tgctgctcag ccgccgacag ccgggcgtac aggggcaaga cgtcgaacgt
     2101 tccgtgcccg atcgctcgct gcctgcgttc gtcgcggcgc aacgcgtcgg cgacgtcgtg
     2161 gatctcacgt tcgccactga ggaacaccag cacgtcgcca tcggtgtcgt cgagcaactc
     2221 gtcgac
//
LOCUS       DAORNF010000597         2217 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_36732_length_2217_cov_1.859852, whole genome shotgun sequence.
ACCESSION   DAORNF010000597 DAORNF010000000
VERSION     DAORNF010000597.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36732_length_2217_cov_1.859852"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2217)
                     /locus_tag="PLV68_18530"
     CDS             complement(<1..>2217)
                     /locus_tag="PLV68_18530"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRE03301.1"
                     /translation="MLAFSRAPEESTGIIRPAPRTDEIRVAERELAVLVRALDAAAAG
                     VPGICVVEGEAGIGKTRLVEELAAAAHQRDCVVVWGRSLEGDAAPAYWPWLAALRALR
                     AWCPNRTNTTVDRLVDATGTAPNAPLTDERGELFDGVLRFLGPAHADGCTDHRPDGHA
                     DRPLVIVLEDMQWADAESLELTARLASGLSGEPVLIVVTIRDGNDADRDAVIALLAAV
                     SRRRGTHRVRLVGLDAAGTAALLGHASGGTIDDRVAGAVHARSDGNPFYAIELQRLLD
                     SEGLVDPAAIAAAGIPVGVRDVVRQRLRRIAQPTLDLLRLAAVAGRDIDVALLSRASG
                     RPLDQCLEALEVAIEHRLLTDGGPGTGLRFAHALVREIVADELSSLRRAQIHLVLADA
                     IMAAGNRDDETEIVAEHLCAAVPIGAGGRAAAALDRAADVAIARFAVDSACDLLTRSL
                     ALHQSTGAGAGDELDTLVKLVWALRARGGYSGGLAHYRRGAELARLLGRHDVEVEMQW
                     AEWAANDTACEFDRARPVAIRFREWAASSDDPVVRLAGSTAWAIQCWHDGALAESAAA
                     FQSVANLRSAVAARAGTVSLAAELCLLSTGFGLYLDEQVGWLLDPDAAFTATAESVQD
                     NFGAAMLWALACTSAASAGALDRVERYSRRVLAAEAGETLGFWGSQARMYLGAALTAT
                     GRALEGRPLFNSGHAGYAAAGMHTGAALMLAFAASAEVIAGDLDRACVHLGMAQSEL"
BASE COUNT          336 a          789 c          788 g          304 t
ORIGIN      
        1 gagctcactc tgcgccatgc ccagatgaac gcatgcccgg tcgaggtcgc ccgcgatcac
       61 ctcggcgctc gccgcgaagg cgagcatcag cgcggccccg gtgtgcatgc ctgcggccgc
      121 gtaccccgcg tgaccggagt tgaacagcgg gcgaccttcc agggcgcggc cggtcgcggt
      181 cagggccgcg ccgaggtaca tccgggcctg gctgccccag aaccccaggg tctctcctgc
      241 ttccgcggcg aggacccggc ggctgtagcg ctcgacgcga tcgagagctc ccgcggacgc
      301 cgcactggtg caggccaagg cccagagcat ggccgctccg aagttgtcct gaaccgactc
      361 ggccgtggcg gtgaacgccg catccgggtc gaggagccac ccgacctgct cgtccaagta
      421 cagtccgaac cccgtcgaca acaggcacag ctcggcggcc aacgagacgg tgcccgctcg
      481 tgccgccacg gcggatcgga ggttggccac ggattgaaat gccgccgccg actccgcgag
      541 cgcgccatcg tgccagcact ggattgccca cgcggtggaa ccggccaggc ggaccaccgg
      601 gtcgtcggat gacgcggccc actcgcggaa cctgatcgcc acgggacggg cgcgatcgaa
      661 ctcgcaggcg gtgtcgttgg cggcccattc ggcccattgc atctcgactt cgacgtcgtg
      721 gcgaccgagc agacgtgcca gttcggcgcc acgccgatag tgcgcgagac cgccgctgta
      781 tccaccgcgg gcgcgcagcg cccacaccag cttgaccagc gtgtcgagtt cgtcaccggc
      841 cccggcgcca gtggactggt gcagcgccag tgagcgcgtc aagagatcgc atgccgagtc
      901 cacggcgaag cgggcgatgg cgacgtcggc ggctcggtcg agcgctgccg cggcacgacc
      961 ccccgccccg atcggtaccg cggcgcagag gtgctcggcc acgatctcgg tctcgtcgtc
     1021 gcggttgccg gccgccatga tggcgtctgc cagcaccaga tggatctggg cccggcgcag
     1081 tgagctgagc tcgtccgcga cgatctcgcg gaccagcgcg tgcgcgaagc gcagcccggt
     1141 gccaggtccg ccgtcggtca acaaccggtg ctcgatcgcg acctcgagtg cttccaggca
     1201 ctggtcgagg ggacggccac tcgcgcggct gaggagtgcg acatcgatgt cgcgaccggc
     1261 cacggcggcc aggcggagca ggtcgagcgt cggctgcgcg atccgccgca gccgttgacg
     1321 taccacgtcg cggacgccaa ctgggatacc ggcggcagcg atcgccgcgg gatccactaa
     1381 cccctccgag tcgagcaacc gctgcagctc gatcgcgtag aaagggttgc cgtcggaccg
     1441 ggcatgcacg gcgccggcaa cgcgatcgtc gatcgttccc ccggatgcat ggccgagcaa
     1501 ggccgccgta cccgcagcgt cgaggccgac gaggcggacg cgatgggtgc cccgccgccg
     1561 cgataccgcg gcgagcaggg cgatcaccgc atcgcggtcg gcgtcgttgc catcgcggat
     1621 ggtgacgacg atcagcacgg gctcgccgct gagtcccgac gcgaggcggg ccgtcagttc
     1681 cagcgactcg gcgtcggccc actgcatgtc ctccagcacg atcaccagtg gtcggtccgc
     1741 gtgaccgtct ggacggtgat cggtacagcc gtccgcgtgt gctggtccga ggaaccgcag
     1801 cacgccgtcg aacagttcgc cacgctcgtc ggtcagaggc gcgttcgggg cggttcccgt
     1861 tgcgtcgacc aggcggtcga ccgtggtgtt ggtgcggttg gggcaccaag cgcgaagggc
     1921 gcgcaacgca gcgagccacg gccagtacgc gggagcggcg tcgccttcga gcgagcggcc
     1981 ccagaccacg acacagtcac gttggtgcgc cgcggcggcc agttcttcga ccaggcgggt
     2041 cttgccgatc ccggcttcgc cttctacgac gcagattccc ggtaccccgg ccgccgctgc
     2101 gtcgagcgcg cggacgagca ccgccagctc gcgctcggcc acgcggatct cgtcggtgcg
     2161 cggcgccggc ctgatgatgc cggtgctttc ctcgggggcg cgggaaaagg cgagcat
//
LOCUS       DAORNF010000598         2216 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_36762_length_2216_cov_1.671911, whole genome shotgun sequence.
ACCESSION   DAORNF010000598 DAORNF010000000
VERSION     DAORNF010000598.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36762_length_2216_cov_1.671911"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1151
                     /locus_tag="PLV68_18535"
     CDS             <1..1151
                     /locus_tag="PLV68_18535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03302.1"
                     /translation="REVGIVVDVQGGFAPGGLRFTPAEPSRPLDTRHGARLAAATVTR
                     VETDAPTGTAAALVNLTADAASSPGYVTADACSALATGPQSRSSLNHIAGAATSNLAV
                     VNVDADGSLCLFNQTATHLIVDVQGFFVPTESAEALGYAAVTPVRLLDTRDTPEPAAA
                     GAIVRVETGHAGVAAALVSIAMTDAATAGYVTADRCSVLVPGPQQTSSGNHRAGLASA
                     NLAAVSVDADGAFCIYTSAAVELAVDLQGTFSTDATGGLVPIAPTRVFDQRPPARPAT
                     PTTSCTSVVHIGDSTSVGMISPSYISDPTRRLDAHQPTDGDLRSAFDRGDLARPGQRL
                     QRRRRTQGRGLSRMLGVRARHHRHRQHRGWWRRQPSSADRQDDGGGRR"
     gene            1138..>1366
                     /locus_tag="PLV68_18540"
     CDS             1138..>1366
                     /locus_tag="PLV68_18540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03303.1"
                     /translation="MVGGDPVMWVNVRTGVGGNDAWSNANMQLWNQALAGAVPSYPNL
                     KVYDWNSAMNPACRVRAGWSLILLCWRDCCGA"
     assembly_gap    1367..1533
                     /estimated_length=167
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1576..>1850
                     /locus_tag="PLV68_18545"
     CDS             1576..>1850
                     /locus_tag="PLV68_18545"
                     /inference="COORDINATES: protein motif:HMM:NF016430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HRE03304.1"
                     /translation="MAVDAEQPHVGLKGLPNVDSDARLLDSLVTAWPSLQRTARTLSG
                     SGGVSDGDDLLAEAIARTLPRWRAGRVDDPAAYVYRTMVNLANKRWRR"
     assembly_gap    1851..1919
                     /estimated_length=69
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1920..2125
                     /locus_tag="PLV68_18550"
     CDS             <1920..2125
                     /locus_tag="PLV68_18550"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 region 4 domain-containing protein"
                     /protein_id="HRE03305.1"
                     /translation="TARDETMRALSQLAPRRRAIVVMRFYEDMPLAAIAARLGIEEGT
                     VKSQLSRALEQLRGQLAGRTEHA"
BASE COUNT          313 a          702 c          663 g          302 t
ORIGIN      
        1 agcgcgaggt cggcatcgtg gtcgacgtcc agggcgggtt cgcaccgggc gggctgcgct
       61 tcaccccggc cgagccatcg cgtccgctcg atactcgtca cggtgcccga ctcgccgctg
      121 ccaccgtgac ccgcgtcgag accgacgcac cgactggcac agccgccgcg ctcgtcaacc
      181 tcacggccga cgcggcgagc agcccgggtt atgtcacggc cgacgcgtgc tcggcgctcg
      241 ccaccgggcc gcagtcgcgc tccagcctca accacatcgc cggcgcggcg acgtcgaacc
      301 tggcggtggt caacgtcgat gccgacggtt cgctgtgcct gttcaaccag accgcgaccc
      361 acctgatcgt ggatgtgcag ggtttcttcg taccaaccga gtcggctgag gcgctggggt
      421 atgccgccgt gactcccgtc cgtctgctcg atactcgcga cacacccgaa ccagcggccg
      481 ctggtgccat cgtccgtgtc gagaccggcc acgctggcgt cgccgcggcg ctggtcagca
      541 tcgcgatgac cgatgccgcc acggcgggct acgtcaccgc cgatcgctgc tcggtgttgg
      601 tccccggccc gcaacagacg tcgtcgggca accaccgcgc cgggctcgcc tcggccaacc
      661 tggccgcggt cagtgtcgat gccgacggcg cgttctgcat ctacacgtcg gccgcagtcg
      721 aactcgccgt ggatctgcag ggtacgttca gcaccgacgc caccggcgga ctcgtgccga
      781 tcgcaccgac ccgagtcttc gaccagcgac ctccggcacg gcccgcgaca ccgaccacat
      841 cgtgcacgtc ggtggtgcac atcggcgact cgacctcggt cggcatgatc agcccgagct
      901 acatctccga cccaacgcga cggctcgacg cccaccaacc cacggatgga gatctccgga
      961 gcgcgttcga tcgtggagac cttgcgcggc caggtcaacg cctacaacgt cgccgcagga
     1021 ctcaaggccg cgggctatcg cggatgttgg gtgttcgcgc tcggcaccac cgacaccgcc
     1081 aacatcgcgg ttggtggcgg cgtcagccgt cgtcagcgga tcgccaagat gatggcggtg
     1141 gtcggcggtg acccggtgat gtgggtcaac gtgcgcaccg gagtcggagg caacgacgcg
     1201 tggtcgaacg cgaatatgca gctctggaac caggccttgg ccggcgccgt gccgagctac
     1261 ccgaacctga aggtgtacga ctggaactcg gcgatgaacc cggcctgcag agttcgagct
     1321 ggctggtcgt tgatcctgct ttgctggcgc gactgctgcg gggcgannnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntccgttc gacaccggcg acgtcgcgat
     1561 cgtcaccgtg acaccgtggc cgtcgacgcc gagcaacctc acgtcgggct gaagggtcta
     1621 ccgaacgtgg actcggacgc tcgactgctc gactcgttgg ttaccgcatg gccgtcgttg
     1681 cagcggacgg cgaggacact gagtggctcg ggcggagtgt ccgatggcga cgacctgctg
     1741 gccgaggcca tcgcccgcac gttgccacgc tggcgcgccg gccgggtgga cgacccagcg
     1801 gcttacgtgt accgcaccat ggtcaacctg gccaacaagc ggtggcggcg nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc
     1921 gaccgcacgc gacgagacga tgcgcgccct gtcgcagctc gcaccgcggc ggcgggcgat
     1981 cgtggtgatg cgcttctacg aggacatgcc gctggccgcg atcgctgccc ggctcggcat
     2041 cgaagagggc acggtcaagt cgcaactctc ccgcgccctc gagcaacttc gcggtcaact
     2101 cgcgggtcgt acagagcatg cgtgacgaac tggaacaacg tctccgccgc gacctgacgt
     2161 ggctcgatcg agtcggcgca ggtgacggcg acccgcacgc agcagcccca cgggtg
//
LOCUS       DAORNF010000599         2214 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_36806_length_2214_cov_1.617879, whole genome shotgun sequence.
ACCESSION   DAORNF010000599 DAORNF010000000
VERSION     DAORNF010000599.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36806_length_2214_cov_1.617879"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>486
                     /locus_tag="PLV68_18555"
     CDS             <1..>486
                     /locus_tag="PLV68_18555"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRE03306.1"
                     /translation="VWRITARAGVKFHDGTDFDGAAIVDNISRHKVGLLTGVAIKSVT
                     DTSLDPDDPMTAIITVEKPWATFPLYVAGQVGYMASPTWLAASDDDATLRSRPVGTGP
                     FIFDDYKANEYFKAHKNPDYWNKPYPYLDSVEFVPIADALQRRDALKAGDVDLMHTTN
                     GS"
     assembly_gap    487..519
                     /estimated_length=33
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <520..1334
                     /locus_tag="PLV68_18560"
     CDS             <520..1334
                     /locus_tag="PLV68_18560"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRE03307.1"
                     /translation="TSDRSETAYALLHVTKEGSILTDQRIRCALAYAYNADTVNEEIN
                     YGVNRLANGPFSPGQVGFLEDSGYPLEQDMAKAQELIAAYKADHPGERLTLALATTND
                     STNLVIANFQKQWWEEAGIDEVTIDQIDQGNYIVTALLGNFEVFQWRNHGGVDLDSQY
                     FWWHSSSSLAVGTLALNFGRIKDANLDDLLDRNRASTDPAEKKQIAEDVNRLFAEECY
                     NLWTTWTVWQVDGNKNIQGIDGQNLVFPDGSTGQSGAGIAGTFFPATLWLDS"
     assembly_gap    1386..1402
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1445..>2214
                     /locus_tag="PLV68_18565"
     CDS             1445..>2214
                     /locus_tag="PLV68_18565"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRE03308.1"
                     /translation="MRYVGQRVIQLLIVVLAVTFTVSLALSAMPNAKERVILSRGAGL
                     TDEVAKQKLLDELHLNDSIPTQYVYFVKDLVSGNWGVTNQNEAVTSSIWSGLGVSVKL
                     MIYGQLIALLIAIPVAINAAYRGGGLFDRMSTTTAFGFLATPAYILAPVLILLLAVRW
                     QIFPARSKNIPIGQVWEHFRNFFLPSITLAIPLAAGYMRLLRADMVLTLQSDFITTAR
                     AKGMSTFRILFGHALRPSLFSLLTSAAINVGTLVGGAL"
BASE COUNT          409 a          781 c          618 g          356 t
ORIGIN      
        1 cgtctggcgg atcaccgccc gcgccggagt caagttccac gacggcaccg acttcgacgg
       61 cgcggcgatc gtcgacaaca tcagccgcca caaggtgggt ctgctgaccg gggtggcgat
      121 caagtcggtc accgacacct cgctcgatcc cgacgacccg atgaccgcga tcatcaccgt
      181 cgaaaagccg tgggcgacgt tcccgctgta cgtcgctgga caggtcggct acatggcctc
      241 acccacgtgg ctggccgcgt ccgacgacga cgccaccctg cgcagccgcc cggtcggaac
      301 cggcccgttc atcttcgacg actacaaggc caacgagtac ttcaaggccc acaagaaccc
      361 ggactactgg aacaagccgt acccgtacct cgacagcgtc gagttcgtgc cgatcgccga
      421 cgcgctccag cgccgtgatg cgctcaaggc gggcgacgtc gacctgatgc acaccaccaa
      481 cggctcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna gacgagcgat cgctccgaga
      541 ccgcctacgc cctgttgcac gtcaccaagg aaggctcgat cctgaccgac cagcgcatcc
      601 gttgcgccct ggcctacgcc tacaacgcgg acacggtcaa cgaagagatc aactacggcg
      661 tcaaccgctt ggccaacggc ccgttctcgc ccggccaggt cggcttcctc gaagacagcg
      721 gctacccgct cgaacaggac atggccaagg cccaagagct gatcgccgcc tacaaggccg
      781 atcaccccgg cgagaggctg accctcgccc tggcgaccac gaacgactcg accaacctgg
      841 tcatcgccaa cttccagaag cagtggtggg aagaggccgg catcgacgag gtcaccatcg
      901 accagatcga ccagggcaac tacatcgtca ccgccctgct cggcaacttc gaggtgttcc
      961 agtggcgtaa ccacggtggc gtcgacctcg actcgcagta cttctggtgg cactccagct
     1021 cgtcgctggc ggtcggcacc ctggccctca acttcggccg catcaaggat gccaacctcg
     1081 acgacctgct cgaccgcaac cgggcctcga ccgacccggc cgagaagaag cagatcgcgg
     1141 aagacgtcaa ccgcctgttc gccgaggagt gctacaacct gtggaccacc tggacggtgt
     1201 ggcaggtcga cggcaacaag aacatccagg gcatcgacgg ccagaacctc gtgttccccg
     1261 acggcagcac cggccaaagc ggcgctggca tcgcaggcac gttcttcccc gccaccctct
     1321 ggctcgatag ctgagccgga gcttccacct cccgtgactg ggtgggccgg ccggattcgt
     1381 tccggnnnnn nnnnnnnnnn nncacagatt ccacgagaga caccggaaag gtcggacccg
     1441 tcagatgcgt tacgtcggac aacgtgtcat ccagctcctg atcgtcgtgc tcgccgtcac
     1501 cttcacggtg tcgctcgcgc tcagcgcgat gcccaatgcc aaagagcgcg tcatcctgtc
     1561 ccgcggtgcg ggcctgaccg atgaggtcgc caagcagaag ctgctcgacg aactccacct
     1621 caacgactcg atccccaccc agtacgtcta cttcgtcaag gacctggtca gcggcaactg
     1681 gggtgtcacc aaccagaacg aggccgtgac ctcctcgatc tggagtggtc tcggcgtgtc
     1741 ggtcaagctg atgatctacg gccaactgat cgcgctgctg atcgcgatcc cggtggcgat
     1801 caacgccgcg tatcgcggtg gcggcctgtt cgaccgaatg tcgaccacca ccgcgttcgg
     1861 cttcttagcc acccccgcct acatcctcgc ccccgtgctc atcctgctgc tcgccgtgcg
     1921 atggcagatc ttcccagccc ggtcgaaaaa cattcccatc ggtcaggtgt gggagcactt
     1981 ccgcaacttc ttcttaccgt cgatcacgct ggcgatccca ctcgcggccg ggtacatgcg
     2041 actgctgcgg gccgacatgg tcttgacgct gcagtccgac ttcatcacca ccgcccgggc
     2101 caagggtatg agcaccttcc gcatcctgtt cggccacgcc ttgcgcccgt cgctgttctc
     2161 gctgttgacg tccgccgcga tcaacgtcgg caccctcgtc ggcggggcgc tgat
//
LOCUS       DAORNF010000600         2214 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_36807_length_2214_cov_1.611394, whole genome shotgun sequence.
ACCESSION   DAORNF010000600 DAORNF010000000
VERSION     DAORNF010000600.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36807_length_2214_cov_1.611394"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2128
                     /locus_tag="PLV68_18570"
     CDS             <1..2128
                     /locus_tag="PLV68_18570"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRE03309.1"
                     /translation="SENYRPVDENGNWTDGCKTGPTGADNAMDIIAFSSRGPSPGQRI
                     KPEVIAPGTHITGTRGNPSDGTTVCDSARPLGNATYAASSGTSHSTPAISGVASLAWW
                     WIANGEGALAFDGGSPSAPSPALMKAYLIAHPTYLTGVSGNGNLPTNAQGYGMPNLQA
                     MFSETPTFLVNQTRVLGATGETWNWVGSAVDPTKPVRIALVWTDAAGAIGTSPQVNNL
                     DLRVEYGSSTYLGNVFTGQWSSTGGTADIRNNYEAVFIQPGSPDAINITVTGFNIAGD
                     GVPGNADTTDQDFAIVCSNCAQTPTFTLGVDPTELSVCTEDTTSVPFNITAGSILGFS
                     TSPTLSASGNPAGTTTAFSVNPVPLPGNSVLTVGNLGAAAAGTSTITITGTAGSEIKT
                     RDVDLTLFTADPGAFALTSPATGAQNVALSPVLSWAASTQAASYTVEVASDASFNTIV
                     WSGSTTATSVTVGTALNSAATYYWRVTAENECGETAVSQPFSFITVPLPGDCPVGVTP
                     TDVYTTDFEGDNAAWVATTGSGTANWAISGLRTHSGAKAFLAADVAVVSDQLLTSPAV
                     TLPTGQNPLALLFWNHQTIEDSSATACFDGAMLQISTNDGASWTQVPDALLQVGPYQG
                     PISSSFSNPAAGLRAWCGDPQDWTRYVVNLDSYAGQTVKFRFRMTSDSSVGRVPDGFY
                     LDDVKVQACGSLPDVIYKDGFDPAVP"
BASE COUNT          366 a          793 c          722 g          333 t
ORIGIN      
        1 ctcggaaaac taccgtccgg tcgacgagaa cggcaactgg acggacggct gcaaaaccgg
       61 cccgaccggt gccgacaatg cgatggacat catagcgttc tcgtcgcgcg gcccgtcgcc
      121 gggccagcgc atcaagcccg aggtcatcgc cccgggcacg cacatcaccg gcacgcgcgg
      181 caacccgagc gacggcacca cggtgtgcga ttcggcgcgt ccgctcggca atgccaccta
      241 tgcggcctcg tccggcacga gccactcgac gccggccatc tcgggcgtgg cctcgctggc
      301 ctggtggtgg atcgccaacg gcgagggcgc gctcgcgttc gacggcggct cgccgtccgc
      361 gccgtcgccg gcgctgatga aggcctacct gatcgcgcac ccgacctacc tgaccggcgt
      421 gtcgggcaac ggcaacctgc cgaccaacgc tcagggctac ggcatgccga acctgcaggc
      481 gatgttcagc gagacgccga ccttcctcgt caaccagacg cgcgtgctcg gtgcgaccgg
      541 cgagacctgg aactgggtcg gctcggcggt cgatccgacc aagcccgtgc gcatcgccct
      601 ggtctggacc gacgccgccg gcgcgatcgg cacgagcccg caggtcaaca acctcgacct
      661 tcgggtcgag tacggttcga gcacctacct cggcaacgtc tttactggtc agtggagcag
      721 cacgggcggc acggccgaca tccgcaacaa ctacgaggcc gtgttcatcc agcccggctc
      781 gccggacgcg atcaacatca cggtgaccgg cttcaacatc gccggcgatg gcgtgccggg
      841 caatgccgac acgaccgacc aggacttcgc gatcgtctgc agcaactgcg cacagacgcc
      901 gacgttcacg ctcggcgtcg atccgaccga gctcagcgtc tgcacagagg acaccacctc
      961 ggtcccgttc aacatcacgg ccggttcgat cctcggcttc agcacgtcgc cgaccctctc
     1021 ggccagcggc aatccggccg gcacgacgac cgcgttcagc gtgaacccgg tgccgttgcc
     1081 gggcaacagc gtgctgacgg tcggcaacct cggcgccgcg gcggccggca cgtcgacgat
     1141 cacgatcacc ggcacggccg gcagcgagat caagacgcgt gacgtcgacc tgaccctgtt
     1201 cacggccgat ccgggtgcct tcgcgctgac ctccccggcc accggcgcgc agaacgtggc
     1261 cctgagtccg gtgctctcgt gggcggcttc cacgcaggcg gcgagctaca ccgtcgaggt
     1321 cgccagcgac gcctcgttca atacgatcgt ctggtccggg tcgacgaccg ccacctcggt
     1381 gacggttggc accgcgctga acagtgcagc gacgtactac tggcgcgtga ccgcggagaa
     1441 cgagtgcggc gaaacagccg tttcgcagcc gttctcgttc atcacggttc cgctgcccgg
     1501 tgactgcccg gtcggagtga cgccgaccga cgtctacacg acggacttcg aaggtgacaa
     1561 cgcggcgtgg gtggcgacca ccggctccgg cacggcgaac tgggcgatct cgggtttgcg
     1621 cacgcacagc ggcgcgaagg ccttccttgc cgcggacgtc gccgtcgtgt cggaccagct
     1681 gctgacctcc ccggcagtga cgctgccgac cggtcagaac ccgctggccc tgctgttctg
     1741 gaaccaccag acgatcgagg actcgagtgc gaccgcctgc ttcgacggcg cgatgctgca
     1801 gatctcgacc aatgacggcg ccagctggac ccaggtgccg gatgcgctgc tgcaggtcgg
     1861 gccgtaccag gggccgatca gctcgagctt ctcgaacccg gccgccggac tgcgcgcgtg
     1921 gtgtggcgat ccgcaggact ggacgcgcta cgtcgtcaac ctcgacagct atgccggaca
     1981 gaccgtcaag ttccgcttcc gcatgacgag cgattcgtcg gtcggccgcg tgcccgatgg
     2041 cttctacctc gacgacgtca aggtgcaggc ctgcggctcg ctgccggatg tgatctacaa
     2101 ggacggcttc gacccggccg tgccgtaagc ggttcgagcc ctctcgatcg accccgcggt
     2161 ttgcgcggcg gggtcggaaa cccgaagccc gtgactgcaa ggtcgcgggc tttt
//
LOCUS       DAORNF010000601         2208 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_36913_length_2208_cov_1.817928, whole genome shotgun sequence.
ACCESSION   DAORNF010000601 DAORNF010000000
VERSION     DAORNF010000601.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36913_length_2208_cov_1.817928"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(192..311)
                     /locus_tag="PLV68_18575"
     CDS             complement(192..311)
                     /locus_tag="PLV68_18575"
                     /inference="COORDINATES: protein motif:HMM:NF013286.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03310.1"
                     /translation="MSRQTMLDGVQTMLVEKVDGLQRVRDLTDHSTGERPYYS"
     gene            337..813
                     /locus_tag="PLV68_18580"
     CDS             337..813
                     /locus_tag="PLV68_18580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03311.1"
                     /translation="MVMYAVPACLARKNRLAEFQQVLEPGWDNHGLELVERIGRLVVD
                     RQPIDHRRTGPVGVADQQTLGVAEVERGPGEQIDVLGGHDHLQPVDGAHLVAERSFGG
                     DLERQVVTEVLPGVTDDLQPQTQSVGLGLGVAQLDDLLVRGRSDADDRLDSQPSQR"
     gene            complement(862..1656)
                     /locus_tag="PLV68_18585"
     CDS             complement(862..1656)
                     /locus_tag="PLV68_18585"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRE03312.1"
                     /translation="MTETTTNNGRELAGRVALVTGGGRGIGRGISELLAAHGATVAVN
                     YRRDREAAEETVASIHTAGGEAKAYEATVDDAAADAAMVEAIVADFGFVDILVCNAGI
                     ASRGQAVIDTDPGELQRVIATHALAAHHLSRLVLPTMRTRERGDIVMISSVATTYMSA
                     NGAPYNMGKAALEALARTLAHEERPYGIHVNTVAPGLVETDMGIRLARALSGNRELTD
                     LRSFDANAPFGRACQPLDVANVVLWLCSPGSGYVTGQRIECDGGGS"
     assembly_gap    1783..1984
                     /estimated_length=202
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1985..>2208
                     /locus_tag="PLV68_18590"
     CDS             <1985..>2208
                     /locus_tag="PLV68_18590"
                     /inference="COORDINATES: protein motif:HMM:NF016175.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03313.1"
                     /translation="DSGLDHALAIGIAPSLLVGDLDSVSNAGRAWAETHEVEICQHPP
                     LKDSTDTELALAAAVESGVTDVVLLGILLY"
BASE COUNT          311 a          696 c          669 g          330 t
ORIGIN      
        1 cgcgccagcg catcaggcgg gtgtcggcgt tgccaccgcg cgctcgaccg ccaccacgcg
       61 gtcgggtagg cgagcatgcc gatcgcgatg ccgccgatcg ctcgggcggg ccgttgcagc
      121 cgggccagcg accgcccgaa catcgaccgt gcgagcagct gccgtgcgct cacgccggtc
      181 tggcccggcg gtcagctgta gtaggggcgc tcgccggtgc tgtgatcggt gaggtcacgc
      241 acccgctgca agccgtcgac cttttcgacc agcatggtct gcacgccgtc gagcatggtc
      301 tggcggctca tcgagcaacc gtggcagcca ccgcccatgg tcatgtacgc ggtgcccgcg
      361 tgtctggccc gcaagaatcg ccttgccgag ttccagcaag tcctcgaacc gggctgggac
      421 aatcatggcc tcgagctggt cgagcggata ggccggctcg tcgtcgatcg tcagcccatc
      481 gaccacaggc ggacggggcc ggttggggtt gcggatcagc agaccctggg agtcgctgaa
      541 gtcgagcgtg gccccggtga gcagatcgat gtccttggcg ggcacgatca ccttcagccc
      601 gtcgatggtg cgcacctcgt cgctgaacgc agctttggtg gtgatctcga acgacaggtc
      661 gtaacggaag tcctgcccgg cgtcactgat gatctccagc cgcagaccca atcggtcggt
      721 ctcgggctcg gcgtcgcgca gctggatgat ctgctcgtac gcgggcggag tgatgctgat
      781 gatcggctcg acagccaacc cagccaacgg tgacaccgga ctactcacgg cctccaggct
      841 accgacccgg tgcggactgc ctcagcttcc gccgccgtca cactcgatgc gctggccggt
      901 gacgtacccg gaccccgggc tgcacagcca cagcaccacg ttggcgacgt cgagcggttg
      961 acacgcacgc ccgaacgggg cgttggcgtc gaacgagcgc aagtcggtga gctcgcggtt
     1021 gcccgagagc gcccgcgcca ggcggatgcc catgtcggtc tcgaccagcc ccggtgccac
     1081 ggtgttgacg tggatgccgt acggccgctc ttcgtgggcg agggtgcggg ccagcgcttc
     1141 gagcgcggcc ttgcccatgt tgtagggcgc cccgttggcc gacatgtagg tggtggcgac
     1201 cgaggagatc atcacgatgt caccccgctc gcgggtgcgc atggtgggca acaccagccg
     1261 cgacaggtgg tgcgcggcga gtgcgtgggt ggcgatcact cgctgcagtt cgcccgggtc
     1321 ggtgtcgatc acggcctgtc cccgcgaagc gataccggcg ttgcagacca gaatgtcgac
     1381 gaacccgaag tcggcgacga tcgcctcgac catcgccgcg tcggcggcgg catcgtcgac
     1441 cgttgcttcg tacgccttgg cctcaccccc ggcggtatgg atcgaagcga cggtctcctc
     1501 ggctgcctcg cggtcgcgtc gatagttgac ggccacggtc gcgccgtgcg cggcgagcag
     1561 ttcggagata ccgcgcccga tccctcgccc tccaccggtc accagtgcaa cccgtccggc
     1621 gagctcgcgc ccgttgttcg tcgtcgtttc cgtcatccca tactcccgag aaaggccgac
     1681 acatccatcg gcaaccgtcg ccccattctc gcggacctcc catccatcgc ccccaaccga
     1741 accgcgcggg ctagggtgaa cccgaacatc aaccagcacg ggnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnncagatt cgggcctcga ccatgcgctg gcgatcggca tcgcgccgtc gctgctcgtc
     2041 ggcgacctcg actcggtctc caacgcgggg cgagcatggg ccgaaaccca cgaggttgag
     2101 atctgccagc acccgccttt gaaggactcg accgacaccg agttggcact ggcggcagcc
     2161 gtcgaatccg gtgtgaccga cgtggtgctg ctcggcatcc tcctgtac
//
LOCUS       DAORNF010000602         2207 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_36942_length_2207_cov_1.640799, whole genome shotgun sequence.
ACCESSION   DAORNF010000602 DAORNF010000000
VERSION     DAORNF010000602.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36942_length_2207_cov_1.640799"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>395)
                     /locus_tag="PLV68_18595"
     CDS             complement(<1..>395)
                     /locus_tag="PLV68_18595"
                     /inference="COORDINATES: protein motif:HMM:NF018648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HRE03314.1"
                     /translation="KVLLLTLAIVDDIGAIVVIAVFYTDRVEFKFVLSAAVIVAAVAL
                     MHRGRVVYPPLIAIAGFALWLAVYESGVHATIAGVIMGLLTPARPIQSELEAEELVNV
                     LENDDDIRADEVRATATLIRGSVSACDRL"
     assembly_gap    396..417
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<418..926)
                     /locus_tag="PLV68_18600"
     CDS             complement(<418..926)
                     /locus_tag="PLV68_18600"
                     /inference="COORDINATES: protein motif:HMM:NF018648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HRE03315.1"
                     /translation="MTTPQIASAQPTFLHGDATLARAVRPLVRFSHIEAAGGIVLVAA
                     TVVALVWANSPWQAGYESLWATNVRFEFGSYVFHEDLGHVVNDLLMAVFFFVVGMEIK
                     RELVVGELRDRRAVALPAMAALGGMIVPAAIFLAFNAGGDGAHGWGIPMATDIAFALG
                     VVALLGSRVP"
     gene            complement(1173..1256)
                     /locus_tag="PLV68_18605"
     tRNA            complement(1173..1256)
                     /locus_tag="PLV68_18605"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1220..1222),aa:Leu,seq:taa)
     gene            complement(1270..1587)
                     /locus_tag="PLV68_18610"
     CDS             complement(1270..1587)
                     /locus_tag="PLV68_18610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03316.1"
                     /translation="MQSANAIEFARAARTLSAEAQRRGLVAPSFRAPPRIVGVDRSLR
                     RHASGVTVAVKLRGRPWAAVLADMVEGVVVANGLEPARAFRLRGELWDALGFTAPITA
                     RVA"
     assembly_gap    1839..1975
                     /estimated_length=137
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1976..>2207
                     /locus_tag="PLV68_18615"
     CDS             <1976..>2207
                     /locus_tag="PLV68_18615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HRE03317.1"
                     /translation="EPDESVIGAAFYLMEPVSGYNVTLGLPPAFQGRPDLQLEMGLSM
                     ADAIAALGRLDPTERGVADIGRWDNWPERQVPR"
BASE COUNT          378 a          670 c          691 g          309 t
ORIGIN      
        1 atcagccgat cgcatgcgga gacggatccg cggatcagcg tggcggtcgc gcggacttcg
       61 tcggcgcgga tgtcgtcatc gttctcgagg acgttgacca gctcctcggc ctcgagttcg
      121 gactggatcg gtcgcgccgg ggtgagcagg cccatgatga cgccagcgat cgttgcgtgg
      181 acgcccgact cgtagacggc gagccacagc gcgaacccgg cgatcgcgat gagcggtgga
      241 tacaccacac ggccacggtg catcaacgcc accgcggcga cgatcacggc cgcggagagc
      301 acgaacttga actcgacacg gtcggtgtag aagacagcga tcacgacgat ggcaccgatg
      361 tcgtcaacga tcgcaagggt caacaacagc accttnnnnn nnnnnnnnnn nnnnnnnggc
      421 acgcgactgc cgagcagcgc gacaacaccg agcgcgaacg caatgtccgt cgccatcggg
      481 atgccccaac cgtgcgcgcc gtcgccgcca gcattgaacg caaggaagat cgcggccggc
      541 acgatcatcc cgcccagtgc agccatcgcc ggcagcgcaa cagctcgccg atcacggagt
      601 tcgccgacga ccagttcccg tttgatctcc atcccgacaa cgaagaagaa gaccgccatc
      661 aacaagtcat tgaccacatg gccgagatcc tcgtggaaga cgtacgaccc gaactcgaac
      721 cgcacgttgg tcgcccacag cgactcgtac ccggcctgcc acggcgagtt cgcccacacc
      781 aacgcaacga ccgtcgccgc tacgagcacg atcccgccgg cagcttcgat gtgagagaag
      841 cggacgagcg gtcgcaccgc tcgggcgagc gtcgcgtcgc cgtggaggaa cgtcggctgt
      901 gcgctggcga tctggggggt tgtcatcggg ggaactcctc ggggtcgacg gaaccacgtc
      961 gaccagactt cccgacacaa ccgaacggaa gtctaagggc aggctccccg gcgcgctcgg
     1021 ctcatcgccg ggtctcgccc gggtggcgct caacggaatc gcccgtccga tttccgctag
     1081 tccgtagaag tttgtgcagg aatcgtgcag tgcaacctcg acctgcatcg ctcttgtcag
     1141 cgcgaatgcc ttgctgacct ggtctttcgt cgtgcccggg acggggttcg aacccgtacg
     1201 tacgttcgta caagggggtt taagcccccc gcgtctgcct atttcgccac ccgggcatgg
     1261 ggtgagacgt tatgcgacgc gggcggtgat gggggcggtg aaaccaaggg catcccacaa
     1321 ctcgccgcgg aggcggaagg cgcgggctgg ttcgaggccg ttggcgacga ccacgccttc
     1381 gaccatgtcg gccaacaccg ctgcccacgg gcggccgcgg agcttgaccg cgacggtgac
     1441 gccgctggcg tgccggcgca gcgagcgatc aaccccgacg atgcgtgggg gagcgcgaaa
     1501 gctgggggcg acgagacccc gacgttgagc ctcggcgctg agcgttcggg ccgcccgggc
     1561 gaactcgatg gcgttggcgg actgcatgag ggcagtgtgc aacaggggtg cgcgatggtt
     1621 tcctcagcgc cgtcgtttcg ggggacaccg ccagctttgc catgatcggt acccatgcag
     1681 gacaacccag ctcgcggcaa catcgaggat gtgaacgtcg tcgaccccga ggtcgtcgat
     1741 ctcgccaaga tcgcggcgtg gatggacgac cacggcctgg aatcggggcc gatcaccgac
     1801 gtcgagttga tcaccggcgg cacgcagaac gtgttgctnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnccgaa
     1981 ccggacgagt cggtgatcgg cgcggccttc tacctgatgg agcccgtcag cgggtacaac
     2041 gtcacgctcg gtctgccgcc cgcgttccag ggccggcccg atctccagtt ggagatgggc
     2101 ttgtcgatgg ccgacgcgat cgccgcgctg ggacggctcg acccgaccga gcgcggcgtg
     2161 gccgatatcg ggcggtggga caactggccc gagcgtcagg tgccacg
//
LOCUS       DAORNF010000603         2204 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_37007_length_2204_cov_1.626803, whole genome shotgun sequence.
ACCESSION   DAORNF010000603 DAORNF010000000
VERSION     DAORNF010000603.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37007_length_2204_cov_1.626803"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="PLV68_18620"
     CDS             complement(<1..546)
                     /locus_tag="PLV68_18620"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRE03318.1"
                     /translation="MIAHYDFPLDPFQLQAIDALDNGRSVLVAAPTGSGKTVVAEHGI
                     ELAMRAGRRAFYTAPIKALSNQKYHDLVARYGPDRVGLLTGDNAINPDAPVVVMTTEV
                     LRNMIYGRTRQLDSLAVAVLDEVHFLQDAYRGPVWEEVIIHLPTHVQLVCLSATVSNA
                     DELTDWITTVRGPTTAVVEDRR"
     gene            complement(548..>1347)
                     /gene="tatC"
                     /locus_tag="PLV68_18625"
     CDS             complement(548..>1347)
                     /gene="tatC"
                     /locus_tag="PLV68_18625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HRE03319.1"
                     /translation="AKRPKLHTPDDRMTLRDHLAELRMRIVRSCLAIIIGVIVIMAFY
                     EPVLEFLSQPYRDLCDRRPKMHCDGRLSTLSALDGFSTRITISLYGGILLALPVILWQ
                     IWRFVVPALHAKEKKYAIPFIFSTVFLFLLGGAIAYWTLELALEFLISWSGGTDQVTQ
                     LFEIKRYMSLVGLMIAAYGIGFEFPVLLVFLQLVGVLSPQTLVRGWRYAVMVIFVIAA
                     VITPSGDPFSMLALAIPMTIFYFIAVLIGWLIQRNRRAKEATEDAAG"
     assembly_gap    1348..1531
                     /estimated_length=184
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1532..1864)
                     /locus_tag="PLV68_18630"
     CDS             complement(<1532..1864)
                     /locus_tag="PLV68_18630"
                     /inference="COORDINATES: protein motif:HMM:NF014470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HRE03320.1"
                     /translation="MFNLTGSEIIFLVVAALVVLGPDKLPEAMRKAGKAYAEFKKLSN
                     SFQSEMRSVIDEPLRELRETADIAKQAAMFDTKPFTDSVKEFGSTLTGGAAVAATPVN
                     PATNTTPAP"
     gene            1931..>2204
                     /locus_tag="PLV68_18635"
     CDS             1931..>2204
                     /locus_tag="PLV68_18635"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HRE03321.1"
                     /translation="MQQRLPSLLQHPIAFAHRGGKAHAPENTIEAFTLGLQMGATGIE
                     SDVWLTSDGVPVLDHDGEVRIRLAKKRIPDLRRDQLPTYIPTLEEML"
BASE COUNT          367 a          646 c          671 g          336 t
ORIGIN      
        1 ccgccggtct tcgaccaccg cggtggtcgg gccgcgcacg gtggtgatcc agtcggtcag
       61 ctcgtccgcg ttcgacacgg tcgcggacag gcacaccaac tgcacgtggg tgggcaggtg
      121 gatgatgacc tcctcccaca ccgggccccg gtacgcgtcc tgtaagaagt ggacctcgtc
      181 caggacggcc acggccagcg agtcgagctg gcgcgtccgg ccgtagatca tgttgcgcaa
      241 cacctcggtg gtcatcacca cgaccggagc gtcggggttg atcgcgttgt ccccggtcag
      301 cagtccgacc cggtcggggc catagcgggc caccaggtcg tggtacttct ggttcgacag
      361 cgccttgatc ggtgccgtgt agaacgcccg ccgtccggcc cgcatcgcca gctcgatgcc
      421 gtgctcggcc accaccgtct tgcccgaacc ggtcggggcc gcaaccagca ccgaacggcc
      481 gttgtcgagc gcgtcgattg cttgcagttg gaacgggtcg agagggaagt cgtagtgggc
      541 gatcaactca gcctgccgcg tcctcggttg cctccttggc gcggcggttg cgctggatca
      601 accagccgat gagcacggcg atgaagtaga agatcgtcat cgggatcgcc agtgcgagca
      661 tcgagaacgg atcacccgac ggggtgatca cggccgcgat cacaaagatg accatcaccg
      721 cgtagcgcca gccgcgcacc aacgtctgcg ggctgagcac gccgacgagc tgcaagaaca
      781 ccagcagcac cgggaactcg aagccgatgc cgtaggccgc gatcatcagt ccgaccaggc
      841 tcatgtagcg cttgatctcg aacagttggg tcacctggtc ggtgccgccc gaccaggaga
      901 tcagaaactc cagcgccagc tcgagggtcc agtacgcgat cgctccgccg agcaggaaca
      961 ggaacaccgt cgagaagatg aacgggatcg cgtacttctt ctccttggcg tgcaacgcgg
     1021 gcacgacaaa gcgccagatc tgccacagga tgaccggcag tgcgagcagg atgccgccgt
     1081 acagcgagat cgtgatgcgg gtcgagaacc cgtcgagcgc gctgagtgtc gacagcctgc
     1141 cgtcgcagtg catcttcgga cgccggtcac acaggtctcg gtacggctgg ctgaggaact
     1201 ccagcacggg ctcgtagaag gccatgatca cgatgacgcc gatgatgatc gccaggcatg
     1261 accggacgat ccgcatgcgc agttcggcca ggtgatcgcg cagcgtcatc cggtcgtcgg
     1321 gagtgtgcag cttggggcgc ttggcgcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngggcgctgg ggtcgtgttg gtcgctgggt
     1561 tcactggggt cgctgcgact gcagcgccgc cggtcaacgt ggacccgaac tccttcaccg
     1621 agtcggtgaa cggcttggtg tcgaacatcg cggcctgttt ggcgatgtcg gcggtctcgc
     1681 gcagctcgcg caacggctcg tcgatcaccg agcgcatctc cgactggaag ctgttcgaca
     1741 gtttcttgaa ctcggcgtag gccttaccgg ccttccgcat cgcctcgggc agtttgtccg
     1801 ggccgagcac gaccaaggca gcgacgacga ggaagatgat ctcgctgccg gtcaggttga
     1861 acacgtcgtg agtctacggg ccagccgtgt ctggcaagag ggcgtcgcca tgcaacaaga
     1921 tgggggtgcc atgcaacaac gtctgccgtc cctgcttcag catccgatcg ctttcgccca
     1981 ccgcggcggc aaggctcatg cgccggagaa cacgatcgag gcgttcacgc tcggcctgca
     2041 gatgggagcg accggcatcg agagcgacgt ctggctgacc agcgacggcg tccccgtgct
     2101 cgatcacgac ggtgaggtgc gcatccgcct ggccaagaag cgcatccccg atctacgccg
     2161 agaccagttg cccacctaca tcccgacgct ggaggagatg cttg
//
LOCUS       DAORNF010000604         2198 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_37147_length_2198_cov_1.907140, whole genome shotgun sequence.
ACCESSION   DAORNF010000604 DAORNF010000000
VERSION     DAORNF010000604.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37147_length_2198_cov_1.907140"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="PLV68_18640"
     CDS             complement(<1..280)
                     /locus_tag="PLV68_18640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03322.1"
                     /translation="MDATLDALDERAMQVASGLLDRSLPKAEWTHEAHVLACIALVRS
                     LGARDALAALRAGIPPYNEATGVANTPTGGYHDTITVYYVWAVAGLVAR"
     gene            complement(351..>803)
                     /locus_tag="PLV68_18645"
     CDS             complement(351..>803)
                     /locus_tag="PLV68_18645"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HRE03323.1"
                     /translation="TLVGFGLRSVAVRTWDGDVVYHTASAIRTFRNSSKHDSRATVDI
                     DIPSTVRVARATSVLQAVCRQAYDSTFRTEPQVLGVIEQRIDRYVVRVSCFVDPASQR
                     EAQYRLKAAAVDAVAELIDGEGVVRDTAQVLAIVSPLPPPETPRVASR"
     assembly_gap    804..894
                     /estimated_length=91
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<895..1179)
                     /locus_tag="PLV68_18650"
     CDS             complement(<895..1179)
                     /locus_tag="PLV68_18650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03324.1"
                     /translation="MIAALTLTHWAVVTAVATVAGVVGLQVAIRFNARSATLAARRRT
                     GLMQLGRWGLLSAGVSALAVEGAVAAGVSSAWTGAAALAFVSCVALAAREP"
     gene            1453..>1768
                     /locus_tag="PLV68_18655"
     CDS             1453..>1768
                     /locus_tag="PLV68_18655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HRE03325.1"
                     /translation="MIAFDEQARRGLEAGMNKLADAVRVTLGPKGRNVVLDKKWGAPT
                     ITNDGVSIAKEIELEDPYERIGAELVKEVAKKTDDVAGDGTTTATVLAQAMVREGLRN
                     VAA"
     assembly_gap    1769..1804
                     /estimated_length=36
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1805..>2198
                     /locus_tag="PLV68_18660"
     CDS             <1805..>2198
                     /locus_tag="PLV68_18660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HRE03326.1"
                     /translation="KKGIEAAVAAAVDSIKALAKEVDSKDQIAQVAAISSADTEIGSM
                     ISDAIDKVGKDGVITVEESQTFGMEMDLVEGMRFDKGYISPYFVTDTDRMEVSLDDAY
                     LLIVSSKISSVRDMLPVLEKVMQSGRPLV"
BASE COUNT          379 a          708 c          703 g          281 t
ORIGIN      
        1 cgcgggcgac gagcccggcg acggcccaca cgtagtacac ggtgatcgtg tcgtggtagc
       61 cgcccgtggg ggtgttggcc acaccggtcg cctcgttgta gggagggatc cccgcccgca
      121 gtgcggccag cgcgtcgcgc gcgccgagcg aacgcaccag ggcaatgcac gccagcacgt
      181 gggcctcatg ggtccactcg gccttgggca agctgcggtc gagcaacccc gacgccacct
      241 gcatcgcccg ctcgtccagg gcatcgaggg tggcgtccat cgacccagct tggcagcgca
      301 gccgccaccg cccgcagtgc atttccgggg gcgcgacggg ccagttcaga tcagcggctg
      361 gcgacgcgcg gcgtctccgg cggcggcaac gggctgacga tggcgaggac ttgggcggtg
      421 tcgcgcacga cgccttcgcc gtcgatcagc tcggccaccg cgtcgaccgc cgcagccttc
      481 agccgatact gggcttcgcg ctggctggcc gggtcgacga agcaggagac gcgcaccacg
      541 taccgatcga tgcgctgttc gatcacgccg agcacctgtg gctcggtgcg aaaggtggag
      601 tcgtaggcct ggcgacacac cgcctgcagc accgacgtcg ctcgggcgac gcgcaccgtc
      661 gaggggatgt cgatgtcgac cgtggcccgc gagtcgtgct tggacgagtt gcggaaggtg
      721 cggatcgcgg aggcggtgtg gtacacgacg tcaccgtccc aggtgcgcac cgccaccgag
      781 cgcaggccga agcccaccag ggtnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncggttc
      901 gcgtgccgcc aaggcgacgc acgacacgaa cgccagcgcc gctgcaccgg tccacgcgct
      961 ggacacgccg gccgcgaccg caccctcgac cgccagcgcg ctgacgcccg cgctcaacag
     1021 gccccaccgg ccgagttgca tcaacccggt gcggcggcga gcggcgaggg ttgccgagcg
     1081 ggcgttgaac cggatggcga cctggaggcc gaccacgccg gccaccgtcg ccactgcggt
     1141 gacgacagcc caatgagtga gggtgagcgc ggcgatcacg aacgagcccc aggtaccagc
     1201 gtggtgatca gcgtggcgaa catcatggcg accattgcac cagcgccacc gcgccaggcg
     1261 cgagcgaccg gatgccaatc gtcctttcgg tcgaactggt caggcgtcac aaacgcacga
     1321 gcatgtaagg cgcgctcgtt cagaccgatc cggttgtcgc gttagcactc gcaccactag
     1381 agtgctaaac gctcgcagct caggcgagac ctatccatca gttcgtccaa cggaggacac
     1441 cagatgccaa agatgatcgc gttcgacgag caagctcgtc gtggcctcga agcgggcatg
     1501 aacaagctcg ccgatgccgt ccgtgtcacc ctcgggccca agggccgcaa cgtggtgctc
     1561 gacaagaagt ggggcgcccc caccatcacc aacgacggcg tgtccatcgc caaggagatc
     1621 gagctcgaag atccgtacga gcggatcggt gccgagctcg tcaaagaagt cgccaagaag
     1681 accgacgacg tcgccggtga cggcaccacg accgccaccg tgctggccca ggccatggtc
     1741 cgcgagggcc tgcgcaacgt cgcggccgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnncaagaa gggcatcgag gccgccgtcg ccgccgctgt cgacagcatc aaggccctgg
     1861 ccaaggaggt cgactccaag gaccagatcg cccaggtcgc cgcgatcagc tcggccgaca
     1921 ccgagatcgg ctcgatgatc tccgacgcga tcgacaaggt cggcaaggac ggcgtcatca
     1981 ccgtcgaaga aagccagacc ttcggcatgg agatggacct ggtcgagggc atgcgcttcg
     2041 acaagggcta catctcgccc tacttcgtga ccgacaccga ccggatggag gtctcgctcg
     2101 acgatgccta cctgctgatc gtgtcgtcga agatcagctc ggtgcgcgac atgctgcccg
     2161 tgctggagaa ggtcatgcag agcggccgtc cgctggtg
//
LOCUS       DAORNF010000605         2197 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_37175_length_2197_cov_1.453782, whole genome shotgun sequence.
ACCESSION   DAORNF010000605 DAORNF010000000
VERSION     DAORNF010000605.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37175_length_2197_cov_1.453782"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="PLV68_18665"
     CDS             <1..373
                     /locus_tag="PLV68_18665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HRE03327.1"
                     /translation="GTPVNFPVIGDDDRKVSDLYDMIHPNANDTLTVRSVFIVGPDKK
                     VKLSLTYPASTGRNFNELLRVIDSLQLTANHKVATPGNWEDGDDVIIGSGVSDDEAKT
                     LFPQGWTTVKPYLRTVPQPGK"
     gene            complement(471..>777)
                     /locus_tag="PLV68_18670"
     CDS             complement(471..>777)
                     /locus_tag="PLV68_18670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03328.1"
                     /translation="ARGALASVGRTYFDAVLAGVAGGAAAAALGDDADALVWLTAAVD
                     EALAVNDVVAVALAQGAYLQVTGEAHPSGAGEVDRLGAGWRSIIEALPQRHRVGHAS"
     assembly_gap    778..1016
                     /estimated_length=239
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1017..>1359)
                     /locus_tag="PLV68_18675"
     CDS             complement(<1017..>1359)
                     /locus_tag="PLV68_18675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03329.1"
                     /translation="ESEALAVKVRAESRERGDEWAEAMMISLLAGLNLWRGNLDVAQS
                     MAEQARTKFHRLHDGFGLAQALGVLGRAQVARGDANVSRTAETLLSRGELFGESPYPL
                     ASVSGIAMHYGD"
     assembly_gap    1360..1382
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1383..2170)
                     /locus_tag="PLV68_18680"
     CDS             complement(<1383..2170)
                     /locus_tag="PLV68_18680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03330.1"
                     /translation="MRHYGVAQSMLSHDGSADDIAYHLGAAAAIVAELGPIVRVPSDV
                     RDQALTALEAKVARAVDQGNLLATARSAQQAIDLFPDPPSAELAHRIALLRLRRIGAL
                     IGMRDLDRATAENDEVLDAAIADGDREIEAGSRRARGLISQTGGDLATARIRLGEAID
                     LYRELDDRPQLAETLRLRGFLELFGGSLSVAEWLFGEAASLYEALGDRRGLGYIDQHV
                     GWMEFLTGRHDQAEAHLSASIAALGELGDRAGVGWAMGLMAFVK"
BASE COUNT          316 a          721 c          616 g          282 t
ORIGIN      
        1 gggcacaccc gtcaacttcc ccgtcatcgg tgacgacgac cgcaaggtgt ccgacctgta
       61 cgacatgatc caccccaacg ccaacgacac cctcacggtg cgctcggtgt tcatcgtcgg
      121 gccggacaag aaggtcaagc tgagcctcac ctaccccgcc tcgaccgggc gcaacttcaa
      181 cgaactgctc cgcgtcatcg actcgctgca actcaccgcc aaccacaagg tcgccacccc
      241 tggcaactgg gaagacggcg acgacgtgat catcggctcg ggcgtgagtg acgacgaggc
      301 caagaccctg ttcccgcagg gctggaccac cgtcaagccc tacctgcgca ccgtcccgca
      361 gccgggtaag tgagctcgac cagcgctgac cacattcttt cattcggaac gaccggcagg
      421 agaactactt ctgccggtcg tttcgcgcct gcgggtgcgg ccaaccgcgt ctacgacgcg
      481 tgcccgacac ggtgacgttg cggcaacgcc tcgatgatgc tgcgccagcc agcgccgagg
      541 cggtcgacct cgccggcacc cgacgggtgc gcttcaccgg tgacctgcag gtacgccccc
      601 tgggccaagg cgacggcgac gacgtcgttg accgcgagcg cctcgtcgac cgcggcggtc
      661 agccacacca gcgcgtcggc gtcgtcaccc agcgcggccg ccgcggcgcc accggcgact
      721 cctgcgagca ccgcgtcgaa gtacgtgcgc cccaccgagg ccagcgcgcc ccgggcgnnn
      781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnatcg
     1021 ccgtagtgca tcgcgatgcc actgacgctc gccaacggat agggcgactc accgaacaac
     1081 tcgccgcgcg acaacaaggt ctcggccgtg cgggagacgt tggcgtcgcc gcgcgccacc
     1141 tgagccctgc cgagcacccc gagcgcctga gccaatccga acccgtcgtg cagccggtgg
     1201 aacttggtgc gggcctgttc ggccatcgac tgggccacgt cgaggttgcc gcgccacagg
     1261 ttgagcccag ccaacaacga gatcatcatc gcctcggccc attcgtcgcc gcgctcgcgc
     1321 gattcggcgc gcaccttgac cgccagggcc tccgactcgn nnnnnnnnnn nnnnnnnnnn
     1381 nnaacttcac gaacgccatc aagcccatcg cccatccgac ccccgcccga tcgcccagtt
     1441 cgccgagcgc ggcgatcgac gcactgagat gggcctcggc ctggtcgtgg cggccggtca
     1501 agaactccat ccacccgacg tgctggtcga tgtaccccag cccgcgccga tcacccagtg
     1561 cttcgtacag gctggctgcc tcgccgaaca gccactcggc caccgaaagc gacccgccga
     1621 acaactcgag aaagccgcgc aagcgcagtg tctcggccag ctggggccga tcgtcgagct
     1681 ctcggtagag gtcgatcgcc tcacccaggc gtatccgcgc cgttgccagg tcgcccccgg
     1741 tctggctgat caggcctcgc gcccggcggc tgccggcttc gatctcgcgg tctccgtcgg
     1801 cgatggccgc gtcgagcact tcgtcgttct ccgcggtggc ccgatcgaga tcgcgcatcc
     1861 cgatcagtgc gccgatgcga cgcagacgca acaacgcgat gcggtgggcc agctctgcgc
     1921 tcggtgggtc ggggaacagg tcgatcgcct gctgtgccga gcgggcggtg gcgagcaggt
     1981 tgccctgatc gacggcgcgg gcgaccttgg cctcgagcgc ggtcagggct tggtcgcgca
     2041 cgtcggacgg aacgcgcacg atcggaccga gctcggccac gatcgccgcg gccgcgccga
     2101 ggtggtaggc gatgtcgtcg gcgctgccgt cgtgcgacag catcgactgc gccacgccgt
     2161 agtggcgcat cgccctcacg gtcttggtca gcgtgtt
//
LOCUS       DAORNF010000606         2195 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_37212_length_2195_cov_1.693458, whole genome shotgun sequence.
ACCESSION   DAORNF010000606 DAORNF010000000
VERSION     DAORNF010000606.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37212_length_2195_cov_1.693458"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    214..224
                     /estimated_length=11
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(394..>2195)
                     /locus_tag="PLV68_18685"
     CDS             complement(394..>2195)
                     /locus_tag="PLV68_18685"
                     /inference="COORDINATES: protein motif:HMM:NF012948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HRE03331.1"
                     /translation="RDPAVVSAPALGHVAPGGTAKYTIRLTNTASQPATDIVVYDILP
                     APGDTGTVDSQSGVSRGSEYTVSLHGPVTTTATATPTIAYSASQNPCRPELGVSTDCV
                     DDWTTTAPADLGTVRAVRVTFTGTTLTNQQGVSIIVPVAVPADVVLGDIAYNSAAYAG
                     VVGGAPLAAEPVRIGLLADGSAIAIDTLVQTIFAADDVTTAPDVEVGEPVRWSFVVTN
                     TGTTTLSNVVVTAPGIDPATIDCGDDSNVVDMLEPDASITCVAVTPGAVAGPTTVVGG
                     VTADSVDGVISDSDPAVAVGVTPLVTVVKATQGVDANTPAEAPRVLPGAAVRWTYAVR
                     NVGTVALTNVVITDDVETGIDCGDGTNVIAALAVDATRTCTLDGVAVGGLYANTGTVL
                     ADPITVYDTAGEAVLLATQASDPSHYVANTPSISVTKLIDGNHIATPANAPVVAAGQP
                     VTVSFVVTNDGELDLTDIALVDAALGPITCPQTALAVGASMVCTATATPSGGLHGGTV
                     RATATVTDVAGAASSEEVQDDDAGWYQVLQAEVELPTIPETTPPTQPEVPGPGLPSTG
                     TSVSPLLTLATLVLGLGVVVLVLRRRGGRTASA"
BASE COUNT          328 a          698 c          803 g          355 t
ORIGIN      
        1 agcctgttgc gacgtgtcgt tgctgttgtc gttgctggcc gacgagtcga cgggaaccga
       61 gtcaccgttg ccgcccgcct gggcctgagt cgggccgacc gcggcaaccc ccgcgacgag
      121 caccatcgcc atcgcagtga cgacgcaccg ctgccaccgt cgcgcgcgcg gcgtgcagtg
      181 gtgcgtcgtc ctgctgtggt tcgtgatcgt gtcnnnnnnn nnnngagccg agaccggccg
      241 aattgcccac gtgtcccgat gccgtcggtc gcatcgtcca ccacaggcgt ccgaccaacg
      301 tccaacggcg tccgacaggt tggcgatggg cccatcagtc cattggacgg tgaggggcgc
      361 gcagttctgc cccgtggggc gcgactcagg ctgtcatgcg ctcgcggttc gtccgccgcg
      421 gcgacggagc accaacacca ccacaccgag gcccagtacc agcgtggcaa gggtcagcag
      481 agggctcacc gacgtaccgg tcgacggcaa tcccgggccg ggaacctcag gctgcgtggg
      541 aggggtcgtc tcgggaatgg tcggtagctc gacttcggct tggagcacct ggtaccaacc
      601 ggcgtcgtcg tcctgcacct cctccgatga cgccgctccg gccacatcgg tgaccgtggc
      661 ggtggcgcgc acggtgccac cgtggagacc acccgatggg gtcgcggttg cggtgcagac
      721 catggaggcg ccaacggcca gcgcggtctg ggggcatgtg atcgggccga gcgccgcgtc
      781 aaccagggcg atgtcggtga ggtcgagttc gccgtcgttg gtgaccacga agctcacggt
      841 caccggctgg ccggcggcca ccaccggtgc gttcgctggg gtggcgatgt ggttgccgtc
      901 gatcagcttg gtcaccgaga tcgagggagt gttcgccacg tagtgcgacg ggtcgctggc
      961 ctgggtcgcc aggagcaccg cttcgccggc ggtgtcgtag acggtgatcg ggtcagcaag
     1021 gacggtgccc gtgttggcgt acagtccgcc gacggccacc ccgtcgaggg tgcacgtccg
     1081 ggtggcgtcg acggccaagg cagcgatgac gttggtgcca tcgccacagt cgatacccgt
     1141 ttcgacgtcg tcggtgatga ccacgttcgt gagcgcaacc gtgccgacgt tgcggaccgc
     1201 gtaggtccag cgcaccgctg caccggggag cacccgcggc gcttccgcgg gcgtgttggc
     1261 atcgacgccc tgcgtggcct tgaccacggt gaccagcggg gtgaccccta cggccaccgc
     1321 cgggtcgctg tcggagatca cgccatcgac ggagtctgcg gtgacgccac cgacgactgt
     1381 ggtgggtccg gcgacggcgc cgggggtgac agcgacacag gtgatcgacg cgtccggctc
     1441 gagcatgtcg acgacgttgg agtcgtcgcc acagtcgatg gtggccggat cgatgccggg
     1501 cgcggtcacc accacgttgc tcagcgtggt ggtgccggtg ttggtgacga cgaagctcca
     1561 gcgcaccggc tcgcccacct cgacgtcggg agccgtggtg acgtcgtcgg cggcgaagat
     1621 ggtttggacc aacgtgtcga tcgcgatcgc cgaaccatcg gcgagcaacc cgatacgaac
     1681 gggttcggcc gcgagcgggg cacccccgac gacgccggca taagcggccg agttgtaggc
     1741 gatgtcaccc aacaccacgt ctgctggcac agcgacgggc acgatgatcg agacgccctg
     1801 ctggttcgtg agtgtcgtcc ccgtgaacgt gacgcgcacc gcgcgcacgg tgccaaggtc
     1861 ggcgggagcc gtggtggtcc agtcgtcgac gcagtcggtg ctcaccccga gctcgggtcg
     1921 gcacgggttc tggctggcac tgtaggcaat ggtcggggta gccgtggcgg tggtggtgac
     1981 cgggccatgc aacgacacgg tgtactccga gccccgggaa acccccgact gactgtcgac
     2041 ggttcccgtg tcacccggtg ccggcaggat gtcgtagacg acgatgtcgg tggccggctg
     2101 agatgcggtg ttggtgaggc ggatggtgta cttggctgtt ccgcccggag cgacatggcc
     2161 gagtgcaggt gccgagacga cggcgggatc acgat
//
LOCUS       DAORNF010000607         2193 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_37248_length_2193_cov_2.091207, whole genome shotgun sequence.
ACCESSION   DAORNF010000607 DAORNF010000000
VERSION     DAORNF010000607.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37248_length_2193_cov_2.091207"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="PLV68_18690"
     CDS             <1..242
                     /locus_tag="PLV68_18690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03332.1"
                     /translation="TRVWWPIALVAALASRRARRAVIAAALVPPLLDWVQRRPAIDPA
                     RYTAIRIADDVAYGSGVWQGVLTTRCAAPLRPTIS"
     assembly_gap    246..360
                     /estimated_length=115
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <361..1253
                     /locus_tag="PLV68_18695"
     CDS             <361..1253
                     /locus_tag="PLV68_18695"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HRE03333.1"
                     /translation="PGLVPVVKGNGYGLGRPALMPIAAGLADTIAVGSVYEATDVPAD
                     RTALVLTPHVGPLPEALPGSAVLTVGSVSHVAALAGQRWRGAVVFKLASSMRRFGARP
                     DELAALADAAEQAGLDVAGYSIHLPLAGDDDARVGEIEAWLGRLDPGLSLSVSHLGPA
                     AYAGLRDRHPERRLALRVGSALWHADKTTLHLSADVLDTHPVRAGDRVGYRHTPVGEH
                     GTLVLLAAGSAHGVQPLDGGLSPFHHLRHRVALIEPPHMHTSLVLIPHHLDPPRVGQR
                     LDVQRPLITALVDELTWVDD"
     gene            1246..>2193
                     /locus_tag="PLV68_18700"
     CDS             1246..>2193
                     /locus_tag="PLV68_18700"
                     /inference="COORDINATES: protein motif:HMM:NF019403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HRE03334.1"
                     /translation="MTDEHAAPQRNRSGAPRLIGPDVTRAIGLIGVVVMNYHGYLNGS
                     GAGRHGSFVERVFNTVDGPLTTRFAAVFVLVAGVGVTLLTNRSRVSGDAAAISDDRWR
                     LVRRGTLLYACGAVLEWIWPGTVIFYYGALFVIAAALFTLRSRWLVAIGAAATVIAAG
                     VAWFRVERLNEGDPTSWLDPEPDSPRNLLLRTLFGYTHPLFPWLAFFCAGIVLGRALP
                     LSRPTARLLGLCGAWLVALTYAINAIGLRAVHGDGPSATTWRRLLSTSPWDRGVLYTV
                     GTLGSSLLAFVVVDALAEATATAPPTRWLQHAGQMTLTLY"
BASE COUNT          254 a          769 c          710 g          345 t
ORIGIN      
        1 cgacccgcgt gtggtggccg atcgcgctcg ttgccgcgct cgcgtcgcgt cgtgctcgcc
       61 gcgcggtgat cgccgcggcg ctcgtaccgc cgctgctcga ctgggtgcag cgccgcccag
      121 cgatcgaccc ggcccgctac accgccatcc gcatcgccga cgacgtggcc tacggaagcg
      181 gtgtctggca gggtgtgctc accactcgct gcgctgcgcc gctgcgcccc accatcagct
      241 gaccgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 ggccgggcct ggtgccggtc gtcaaaggaa acggctacgg gctgggccgc ccggcgctga
      421 tgccgatcgc cgcagggttg gccgacacga tcgccgtcgg gtcggtgtac gaagcgacgg
      481 acgttcccgc cgatcgcacc gcgctggtgc tgacacccca cgtcggtccg ctgcccgagg
      541 ccctacccgg atcagcggtg ctcaccgttg gcagcgtcag ccacgtcgcc gcgctggcag
      601 gccaaagatg gcgcggtgcc gtcgtgttca aactggcgtc gtcgatgcgc cgcttcgggg
      661 cgaggccgga cgagttggcc gcgctggccg acgcggccga acaggccggg ctcgacgtcg
      721 ccggttactc gatccacctg cccctcgccg gagacgacga tgcccgcgtg ggcgagatcg
      781 aagcctggct cggccggctc gatccgggtc tgtcgctgtc ggtcagccac ctcggccccg
      841 cggcctacgc cgggttgcgc gaccgccatc ccgagcggcg cttggccctg cgtgtcggct
      901 cggcgttgtg gcatgcggac aagacgacgc tgcacctcag cgccgacgtg ctcgacacgc
      961 atccggtgcg ggccggcgac cgcgtgggct accgccacac tccggtcggt gagcacggca
     1021 cgctcgtgtt gctcgccgcc gggtcggctc acggtgtgca accgctcgat ggcgggctca
     1081 gcccgttcca ccatctccgc caccgcgtcg cgctgatcga gccgccgcac atgcacacgt
     1141 cgctggtgct gatcccccac catctcgacc cgccgcgcgt cgggcagcgt ctcgacgtgc
     1201 agcggccgct gatcaccgcc ctggtcgacg aactgacctg ggtcgatgac tgacgagcac
     1261 gccgcgccgc agcgcaaccg ctctggggcg ccacggctga tcggtcccga cgtcacccgc
     1321 gcgatcgggc tgatcggcgt ggtcgtgatg aactaccacg gctacctcaa cggctccggc
     1381 gccgggcgtc acggttcatt cgtcgagcgg gtgttcaaca cggtcgacgg tccgttgaca
     1441 actcgcttcg cggccgtgtt cgtactggtg gcaggggtcg gtgtgacgct gttgaccaac
     1501 cgcagccgcg tcagcggcga cgctgccgcg atcagcgatg accgctggcg gctggtccgc
     1561 cgcggcacgt tgctgtacgc gtgcggggcc gtactggagt ggatctggcc cggcacggtc
     1621 atcttctact acggggcgct gttcgtcatc gccgccgcac tgttcaccct gcgctcacgt
     1681 tggctcgtcg cgatcggcgc cgcagcaacc gtcatcgcgg ccggtgtcgc atggttccgg
     1741 gtggaacgac tcaacgaggg cgaccccacg tcgtggctcg accccgaacc cgactcgccg
     1801 cgcaacctgt tgctgcggac gctcttcggc tacacccatc cgttgtttcc gtggctggcg
     1861 tttttctgcg cgggcatcgt gctggggcgg gcgctgccgc tgtctcgccc gaccgcacgg
     1921 ctgctcggcc tgtgtggggc atggttggtc gcgctgacct atgcgatcaa tgcgatcggc
     1981 ctgcgcgccg tccacggtga cggcccgtcg gcgaccacct ggcgacggct gctgtcgacg
     2041 tcaccgtggg accgcggcgt gctctacacg gtgggcacgc tcggctcgtc gctgctggcc
     2101 ttcgtcgtcg tcgacgcgct cgccgaggca accgccaccg ccccgccgac ccgctggctg
     2161 caacacgcgg gccagatgac cctcaccctc tac
//
LOCUS       DAORNF010000608         2190 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_37316_length_2190_cov_1.561593, whole genome shotgun sequence.
ACCESSION   DAORNF010000608 DAORNF010000000
VERSION     DAORNF010000608.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37316_length_2190_cov_1.561593"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>951
                     /locus_tag="PLV68_18705"
     CDS             <1..>951
                     /locus_tag="PLV68_18705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03335.1"
                     /translation="FGQIEWVKNERGDTTNYDYRADGRLWKIRYPVGDTTTWNDTVLS
                     FAPTTDGNLGVGGTHWRQTITTGTGRKTTEFDALWRPVVTLEEDTSNASTKRYVTRTF
                     DHAGRETFTSYPSASAGTAGTWTTYDGLGRPTKIEATSELVPSLLTTQIAYDSGFTKT
                     VTNPRGFSTTTSFKAFDTPSEDWPVEIDDPVGLTSIARDAFGKPLSMSRSGSYTYPGT
                     ATPLSVNTTRSYVYDTAQRLCKTVEPESGATILAYDLADNVAWSASGQSLTGTGSCNN
                     TNPVAISGKANFSYDARNRLLVTDYDTPTTPDIVRSYTPD"
     assembly_gap    952..1073
                     /estimated_length=122
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1074..>2190
                     /locus_tag="PLV68_18710"
     CDS             <1074..>2190
                     /locus_tag="PLV68_18710"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HRE03336.1"
                     /translation="VWNANGHAASLSYPDLGAVDYLPNGLGQPTKVGTFASGVTWHPN
                     GAIAGFTYGNGIVHSQSQTTRGLPQVREDTGALQDHYAWDGNGNVAAITDQRIAPPQS
                     RTRSMAYDGRDRLTSTGFPFAGSTFSYSYDPLDNLRTVSATGGRNHRLEYAANGRLTR
                     LYDPASPGMDVIGYAYDARGNTTDRTSATGFVPDQSFVVDEANRVRSTSGRVVQGYAY
                     DGHGRRTRTTTGSNTRVQVYARDGRLLLEDNAARNAKLIKPVSYLYLGNRLLAQQHDD
                     GSRKYVHTDVLGSVVAESTAAPPAITQRPMWEAYGAPWNGSFTHGPGYAGHAMDTATK
                     LVYMQQRYHDPYAGRFLAVDPVAASAGSFNRYWYANNN"
BASE COUNT          396 a          755 c          665 g          252 t
ORIGIN      
        1 ccttcggcca gatcgagtgg gtcaagaacg aacggggcga cacgacgaac tacgactatc
       61 gcgcggacgg ccgcctttgg aagatccggt atccggtcgg tgatacgacg acctggaacg
      121 atacggtgtt gtcgttcgca ccgaccacgg acggcaacct cggcgtcggc ggcacccatt
      181 ggcgccagac gatcaccacc ggcaccggcc gcaagaccac cgagttcgac gccctgtggc
      241 gccccgtggt gaccctggaa gaagacacca gcaacgcctc gacgaagcgt tatgtgacac
      301 gcacgttcga ccacgccggc cgcgagacct tcacatccta cccgagcgcg agtgcgggca
      361 cggccggcac ctggaccacc tacgacggcc tcggccggcc gacgaagatc gaggcgacga
      421 gcgaactggt gccgagcctg ctgacgacgc agatcgccta cgactccggc ttcacgaaga
      481 cggtgaccaa cccgcgcggc ttcagcacca ccacgagctt caaggccttc gacaccccca
      541 gcgaagactg gccggtggag atcgacgacc cggtcggctt gacgtccatc gcccgcgacg
      601 ccttcggcaa gccgctgtcg atgagccgca gcggcagcta cacctatccg ggcacggcaa
      661 ccccgctgtc ggtcaacacc acgcgcagct acgtctacga caccgcccag cgcctgtgca
      721 agacggtcga accggagtcc ggcgcgacga tcctcgccta cgatctggcc gacaacgtcg
      781 cctggtcggc ctcggggcag agcctgaccg gcaccggcag ctgcaacaac accaaccccg
      841 tcgcgatcag cggcaaggcg aacttcagct acgacgcccg caaccggctg ctcgtcaccg
      901 actacgacac cccgaccacg ccggacatcg tgcgcagcta cacgcccgac gnnnnnnnnn
      961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngtctgga
     1081 acgccaacgg ccatgcggcg agcctgagct acccggacct cggcgcggtc gactacctgc
     1141 ccaacgggct cggccagccg acgaaggtcg gcaccttcgc gagcggggtc acctggcacc
     1201 cgaacggggc gatcgccggg ttcacctacg gcaacggcat cgtccacagc cagagccaga
     1261 cgacgcgcgg cctgccgcag gtgcgcgagg acaccggcgc gctccaggac cactacgcct
     1321 gggacggcaa cggcaatgtc gcggcgatca ccgaccagcg gatcgcgccg ccgcagagcc
     1381 gcacgcgcag catggcctac gacggccgcg accgcctgac ctcgaccggc ttcccctttg
     1441 ccggcagcac gttcagctac agctacgacc cgctcgacaa cctgcgcacg gtctcggcga
     1501 ccggcgggcg caaccaccgg ctggagtacg cggccaacgg gcggctgacg cggctgtatg
     1561 acccggccag ccccggcatg gacgtgatcg gttacgccta cgacgcacga ggcaacacga
     1621 ccgaccgcac cagcgccacc ggcttcgtgc cggaccagag cttcgtggtg gacgaggcga
     1681 accgggtgcg ctcgaccagc ggccgggtgg tgcagggcta cgcctacgac ggccacggcc
     1741 ggcgcacgcg cacgacgacg gggtcgaaca cgcgcgtgca ggtgtacgcc cgggacggtc
     1801 gcctgctgct ggaggacaac gccgcgcgca acgccaagct gatcaagccg gtgtcctacc
     1861 tctacctcgg caaccgcctg ctggcgcagc agcacgacga cggcagccgc aagtacgtgc
     1921 acacggacgt gcttggcagc gtggtggcgg agagcacggc cgccccgccg gcgatcaccc
     1981 agcggccgat gtgggaggcc tacggggcgc cgtggaacgg ctcgttcacc cacggccccg
     2041 gctatgccgg gcatgcgatg gacacggcga ccaaactggt ctacatgcaa cagcgctacc
     2101 atgaccccta tgccgggagg ttcctggcgg tggatccggt ggcggccagt gcggggagtt
     2161 tcaatcggta ttggtatgcc aataacaacc
//
LOCUS       DAORNF010000609         2186 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_37404_length_2186_cov_2.004223, whole genome shotgun sequence.
ACCESSION   DAORNF010000609 DAORNF010000000
VERSION     DAORNF010000609.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37404_length_2186_cov_2.004223"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /locus_tag="PLV68_18715"
     rRNA            <1..340
                     /locus_tag="PLV68_18715"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            440..556
                     /gene="rrf"
                     /locus_tag="PLV68_18720"
     rRNA            440..556
                     /gene="rrf"
                     /locus_tag="PLV68_18720"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(627..1802)
                     /gene="mftD"
                     /locus_tag="PLV68_18725"
     CDS             complement(627..1802)
                     /gene="mftD"
                     /locus_tag="PLV68_18725"
                     /EC_number="1.4.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443197.1"
                     /note="MftD, an enzyme found in the mycofactocin
                     biosynthesis locus, performs an oxidative deamination of
                     3-amino-5-[(p-hydroxyphenyl)methyl]-4,
                     4-dimethyl-2-pyrrolidinone (AHDP). The resulting compound,
                     now called pre-mycofactocin (PMFT), is a biologically
                     active redox cofactor that can oxidize the
                     non-exchangeable NADH of TIGR03971 family SDR-type
                     oxidoreductases; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin biosynthesis FMN-dependent
                     deaminase MftD"
                     /protein_id="HRE03337.1"
                     /translation="MGNPWFETVAVAQRRAKRRLPASVYGALVAGSERGLTVADNVAA
                     FDELGFAPFVAGLPQQRDQATTVMGQPISMPVIASPTGVQAVHPDGEVAVARATAARG
                     TAMGLSSFASKPVEEVIAANPQTFFQLYWSGSRDQMVQRMQRAKDAGAVGLIITLDWS
                     FSTGRDWGSPSIPERIDFKAMLQHAPEAILKPKWLLEFAKTKKIPDLTVPNMTDPGGA
                     APTFWAVYGEWMQTPLPTWDDIAWVVAQWDGPVMLKGITRVDEAKRAVGAGVTAISVS
                     NHGGNNLDGTPASIRCLPAVVAAVGSDAEIVLDGGIRRGSDVVKALALGARAVMIGRA
                     YLWGLAANGQAGVENVFDILRGGIDSALMALAKSSVHDLTPADVLVPDGFTRTLGAD"
     gene            1978..>2186
                     /locus_tag="PLV68_18730"
     CDS             1978..>2186
                     /locus_tag="PLV68_18730"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HRE03338.1"
                     /translation="MTDHRAPGRPPGTSARQLELIALDLYSTAGFDQTTVEEIAAAAG
                     VSRRTFFRYFDSKVAVLWHAFDDEVR"
BASE COUNT          395 a          682 c          732 g          377 t
ORIGIN      
        1 taaagcggta cgcgagctgg gttcagaacg tcgtgagaca gttcggtccc tatcctccgc
       61 agccgaaggt gtcttgagga aggctgtccc tagtacgaga ggaccgggac ggacggagct
      121 ctggtgtgtc agttgtcttg ccaaaggcat ggctgattag ccacctccgg aagggataac
      181 cgctgaaagc atctaagcgg gaagcctgtt ccaagatgag tacacccaca cacattgtgt
      241 ggtaaggccc gtggccagac aaccacgttg ataggccgga agtgcacgca cagtaatgtg
      301 ttcagctgac cggtactaat cggccgaggg cttggaccaa aaacccccaa acaggggggg
      361 ggcacaccaa caacgatcga catgttcgta ctcactcttc accactcaaa cacccaacat
      421 gggtgagtgg taccagaact ccggtggcaa cagcgtcagg gacacacccg tacccatccc
      481 gaacacgacc gttaagactg acagcgccaa tggtacttgg gggtagaccc cctgggagag
      541 taggacaccg ccggaacaac actcaccaac aaggcccccg caactgcggg ggccttgttg
      601 ctttttcgga ccagtcgagt tgcggttcag tcggcgccga gggtgcgggt gaagccgtcg
      661 ggtaccagca cgtcggcggg ggtcaggtcg tgtacggacg acttggccag cgccatgagc
      721 gccgagtcga taccaccgcg gaggatgtcg aacacgttct cgaccccggc ctggccgttg
      781 gcggccaacc cccacagata ggcgcgtccg atcatgaccg ctcgcgcacc gagcgcgagg
      841 gccttgacca cgtcgctgcc gcggcggatg ccgccgtcga gcacgatttc ggcgtcggag
      901 cccaccgcgg cgaccacagc gggcaggcag cggatcgagg ccggtgttcc gtcgaggttg
      961 ttgccgccgt ggttcgacac agagatcgcc gtgacgcccg cgccgacggc gcgcttggct
     1021 tcgtcgacgc gggtgatgcc tttgagcatc acggggccgt cccattgggc gaccacccag
     1081 gcgatgtcgt cccacgtggg cagcggggtc tgcatccact cgccgtacac ggcccagaac
     1141 gtcggcgctg cgccccccgg gtcggtcatg ttgggaacgg tgaggtcggg gatcttcttg
     1201 gtcttggcga actcgagcag ccacttcggc ttgaggatcg cttcgggcgc gtgctgcagc
     1261 atcgccttga agtcgatccg ctcggggatc gacggactac cccagtcgcg tccggtcgag
     1321 aacgaccagt cgagggtgat gatcaacccc actgccccgg cgtccttggc gcgttgcatg
     1381 cgctgcacca tctggtcacg gctgccgctc cagtagagct ggaagaacgt ctgggggttt
     1441 gctgcgatca cctcctcgac cggcttgctg gcgaacgagc tgagtcccat ggcggtgccg
     1501 cgggcagcgg tggcgcgggc gacggcaacc tcgccgtcgg ggtgcaccgc ttgtacgcca
     1561 gtgggcgagg cgatgaccgg catcgagatc ggctggccca tcaccgtggt ggcctgatcg
     1621 cgttgttggg gcaatccggc cacgaagggg gcgaaaccca actcgtcgaa cgctgcgacg
     1681 ttgtcggcca cggtgaggcc gcgttcggat ccggcgacca gcgcgccgta caccgacgcg
     1741 ggtaggcgcc gcttggcacg tcgctgcgcg accgccaccg tctcgaacca ggggttgccc
     1801 atctgtcgtt tgcctctcgt cgtcgatgcg tgcatcgcag cggtccggcg attgctgtcg
     1861 gccggatgga tgatcgtgat cgagtgatta tggcacacgg tgtcaaatca tcccagcagc
     1921 aagtgggtag ggatgctccc tcggctcaga ccgtgtcggc ggcgtacagt gcgggccgtg
     1981 acagatcacc gcgcacctgg tcgtccaccc ggaacgagcg cgcggcaact ggagttgatc
     2041 gctctcgatc tgtacagcac cgccgggttc gaccagacca ccgtggaaga gatcgcggca
     2101 gcggccgggg tcagccggcg cacgttcttc cgctacttcg attcgaaggt ggcggtgctg
     2161 tggcacgcct tcgacgacga ggtgcg
//
LOCUS       DAORNF010000610         2186 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_37408_length_2186_cov_1.817926, whole genome shotgun sequence.
ACCESSION   DAORNF010000610 DAORNF010000000
VERSION     DAORNF010000610.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37408_length_2186_cov_1.817926"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>211
                     /locus_tag="PLV68_18735"
     CDS             <1..>211
                     /locus_tag="PLV68_18735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03339.1"
                     /translation="GLLGADHLGVASIAPYVDIDGRWLGDDYAVPTTAADAAIAWAAR
                     SAGLVLDRTYSGKGMAGLLGNVGDGR"
     assembly_gap    212..380
                     /estimated_length=169
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <381..>742
                     /locus_tag="PLV68_18740"
     CDS             <381..>742
                     /locus_tag="PLV68_18740"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HRE03340.1"
                     /translation="KLAAGLMTMGGELIDLAREPVDHDLDATGIFQQLEGLVEQQLER
                     ARSHHEVRPVAVGVGCAGPIAPNCAAVSPLNIRAWHNFPLRDRLMQVSDLPVYGDLDA
                     KALALAEGWVGAAQGRMN"
     assembly_gap    743..787
                     /estimated_length=45
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <788..1241
                     /locus_tag="PLV68_18745"
     CDS             <788..1241
                     /locus_tag="PLV68_18745"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HRE03341.1"
                     /translation="LDGQLLDGATGNAGHVGHVIVEPNGRRCSCGARGCLEAEASGPA
                     IEAITGRPPTEPSYDIMVRTGRLVGRGVASVCNALDIDLVVVGGSVALGFGATFFNAA
                     QESLSEHCRLQFSRSARITPARLGDQGPLIGAGAVGLRGVHRRQRSAP"
     gene            1320..>2186
                     /locus_tag="PLV68_18750"
     CDS             1320..>2186
                     /locus_tag="PLV68_18750"
                     /inference="COORDINATES: protein motif:HMM:NF016143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HRE03342.1"
                     /translation="MTKIRKEGSSSQIQDRRSQSSGGAAGGGLGDLLGGMLGGGGSGG
                     GGGGLGLPTKMGGGLLGIIVMVAALFLPRLLNGAGTQQAVDTRAADGSEAACSTEIEQ
                     IVCGGTEDVQDFWEREFAARGERYTTTQTVFFSGATNTGCGRASSNTGPFFCPADDLV
                     YFDLDFLLQLQSQFKAEGDLAAQYIVAHEYGHYVQDLLGINAEMTRLQQRNPSKANQY
                     SVALELQADCLAGVWANDAAARGQFDSSREINEALNAAEAVGDDRIQQQSQGYVDPDS
                     FTHGTSEQRKTWF"
BASE COUNT          312 a          650 c          703 g          307 t
ORIGIN      
        1 tggggctgct cggtgccgac cacctcggtg tggcgtcgat cgcaccgtat gtcgacatcg
       61 acggacgttg gctgggtgac gactacgcgg tgcccaccac ggccgccgac gcggcgatcg
      121 cgtgggcagc ccgcagtgcg gggctggtgc tcgatcgcac ctattcgggc aagggcatgg
      181 cgggcttgct gggcaacgtg ggcgacgggc gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn caagctggcc gccggtctga tgacgatggg tggcgagctg
      421 atcgatctgg cccgtgaacc ggtcgatcac gacctcgatg ccaccggcat ctttcagcaa
      481 ctggaagggt tggtcgagca gcagttggag cgagcgcggt cgcatcacga ggtgcggccg
      541 gtggccgtcg gggtcggttg tgccgggccg atcgccccca actgcgcggc cgtgtcgccg
      601 ctgaacatcc gggcgtggca caacttcccc ctccgcgacc ggctgatgca ggtcagcgac
      661 ctgccggtgt acggcgacct cgatgccaaa gccctcgcct tggcggaagg atgggtcggc
      721 gccgcccagg gccggatgaa ctnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      781 nnnnnnngct cgacggccaa ctgctcgacg gtgcaacggg caacgccggc cacgtcgggc
      841 atgtgatcgt cgagccgaac ggtcgccgct gtagctgcgg ggcgcggggg tgcctggagg
      901 ccgaagcctc gggaccggcg atcgaagcga tcaccggacg gcctcccacc gagccgagct
      961 acgacatcat ggtccgcaca gggcgcctcg tcgggcgggg cgtcgcctcg gtctgcaacg
     1021 cgctcgacat cgacctggtg gtggtcggtg gttcggtcgc gctggggttc ggggcgacct
     1081 tcttcaacgc cgcgcaggag agcttgtccg agcactgccg actgcagttc agccgcagcg
     1141 cgcggatcac cccggcccgg ctcggtgacc aggggccctt gatcggcgcc ggcgcggtcg
     1201 gtttgcgtgg cgtgcatcgc cgccaacgct cggcaccctg atctggggct gatcatccag
     1261 taccgtctgg gcgtggtgcc gaccaacgat cggcgccgat ccgctggagg acacacccga
     1321 tgaccaagat tcgcaaagaa ggatcctcgt cccagatcca agaccgccgg agccagtcca
     1381 gcggtggtgc ggctggcggc ggtctgggcg acctgttggg tggcatgctc ggtggtggtg
     1441 gaagtggcgg cggaggcggt ggtctgggcc tgccgaccaa gatgggcggt ggcttgctcg
     1501 gcatcatcgt catggtcgcg gcgctgttct tgccccggtt gctcaacggt gctggcaccc
     1561 agcaggccgt cgacacgcgc gcggccgacg ggtcggaggc cgcgtgcagc accgagatcg
     1621 aacagatcgt gtgcggcggc acagaagatg tgcaggactt ctgggagcgc gagttcgccg
     1681 cccgtggcga gcgctacacc acgacccaga ccgtgttctt cagcggggcg accaacaccg
     1741 gatgcggcag ggcgtcgtcg aacaccgggc cgttcttctg ccccgccgac gatctggtct
     1801 acttcgacct cgacttcctg ctgcagctcc agtcgcagtt caaagccgaa ggcgacctgg
     1861 ccgcgcagta catcgtcgct cacgagtacg gccactacgt gcaggacctg ctcggcatca
     1921 acgccgagat gacccggctc cagcagcgga acccgagcaa ggccaaccag tactcggtcg
     1981 cgctggaact gcaggccgac tgcctcgccg gggtgtgggc caacgacgca gccgctcgcg
     2041 gccagttcga cagctcgcgc gagatcaacg aggcgctcaa cgcggccgaa gctgtcggcg
     2101 acgaccgcat ccagcagcag agccagggtt acgtcgatcc cgactcgttc acccacggca
     2161 cgtccgaaca gcgcaagacc tggttc
//
LOCUS       DAORNF010000611         2184 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_37463_length_2184_cov_1.723814, whole genome shotgun sequence.
ACCESSION   DAORNF010000611 DAORNF010000000
VERSION     DAORNF010000611.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37463_length_2184_cov_1.723814"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2171)
                     /locus_tag="PLV68_18755"
     CDS             complement(<1..2171)
                     /locus_tag="PLV68_18755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03343.1"
                     /translation="MATATVRGRLPKSSDPHRILAEAAGRLTRHGSEPGLNPTLRNQV
                     AWMVTDRVGLAAHDSPPEFDPSGLKPRDRWETLQNVLDPARKLTAAFGVAIGVDLVAS
                     PLPRMTIVDDRVVPANRRHRLPAASLDQLPVGQWVDVGPYTAAEWESRGEQASGRGAY
                     LRLNKTAYIVAVEHGDTAAWRLEDVAARTGDGILATGTANSLDDARNDATNTLQGRYP
                     ALDPQRVDVAAPIPAGAIRGWEPMPGEGSTSAELRRLDERTTVYVIPGPGGRWMPAVH
                     HAATGDIDRLPLTRTVDDAKHAAVLAGYRTIRLAAMDTPAKFDPMLAAFADSGDYSRR
                     ELTALLTSRLEAADSARLPDATPADLIELVGAAGASPATTVAILHAEAADPRTVAELL
                     PTVGVPIPDAIRVLHERWEMPRPEAAELLDATAAEMRTAGCTPVEIIAARPRDVLRSL
                     PDEPHLWELAAGTMALAGHSAATIANHLVAHAPSPDAFAAALTAGIDDPTAGVAVAVR
                     SGAQGDQLAAVTEAYGLAPAEAATLLTDHGCQANVLLDTLDIRCDHDTQAVTGLAGHA
                     GIDTAAIEAWMNPTEPIVPPIARWGGLDLGDADELLARLPTPSPTRQTSPPSSTSNPH
                     ATWSRSAHEHRTDQPTHRRTRGRWHLATRPRRRRRLPPPRYPPRHHRLGRARRSPPLA
                     TRRSRHLRHTGRNRHRDRRCHGRPDRRPNLDPNRDPPLGAD"
BASE COUNT          264 a          627 c          908 g          385 t
ORIGIN      
        1 tcgtcagcac ccagcggcgg atcgcggttt ggatcgagat tggggcgtcg gtctgggcgg
       61 ccatggcatc gacgatcgcg gtggcgattg cgtccggtgt gccggaggtg tcggcttctg
      121 cgagtagcca gcggagggct tcgccgagcg cgtcccagac ggtgatgtcg gggcggatac
      181 cgcggcggag gtagtcgacg gcggcgacga ggtcgagtag ccaggtgcca ccttccgcga
      241 gttcgtcggt gagttggttg gtctgttcgg tgttcatggg cggatcggct ccaggtcgcg
      301 tgtgggttcg aggtcgaact cggtggcgag gtctgtcgtg tcggcgacgg ggtggggagg
      361 cgggcgagca gttcgtcggc gtcgccgagg tcgagtccgc cccaccgggc gattggcggg
      421 acgattggct cggtggggtt catccacgcc tcgattgctg cggtgtcgat gccggcgtga
      481 ccggcgagcc cggttacggc ttgggtgtcg tggtcgcagc ggatgtcgag cgtgtcgagg
      541 agaacgttcg cttggcagcc gtgatcggtg agcaacgttg cggcttcagc gggtgcgagg
      601 ccgtaggcct cggtgactgc ggcgagctgg tcgccttggg cgccgcttcg gacagccaca
      661 gcgacgcctg cggtggggtc gtcgatgccg gccgtgagcg ctgccgcgaa cgcatcgggc
      721 gacggtgcat gggcgacaag atggttggcg atcgttgctg ctgagtggcc ggcgagcgcc
      781 atcgtgccgg cggcgagctc ccacaggtgg ggttcgtcgg gcagtgagcg gagtacgtcg
      841 cgtgggcgcg cggcgatgat ctcgacgggg gtgcagccgg ctgtgcgcat ctcggccgcc
      901 gtcgcgtcga gcagttcggc ggcttcgggt cggggcatct cccagcgttc gtggagcaca
      961 cggatcgcgt cggggatggg gacgccgacg gtcgggagga gttcggcgac cgttcgtggg
     1021 tcggctgctt cggcgtgcag gatcgcgacg gtggtcgcgg gggatgcgcc ggcggcgccg
     1081 acgagttcga tgaggtctgc gggtgtggcg tctgggagtc gtgcggagtc ggctgcctcg
     1141 agacggctcg tgaggagggc ggtgagttcg cgtcgggagt agtccccgct gtccgcgaac
     1201 gcggcgagca tggggtcgaa cttggcgggt gtgtccatcg cggcgaggcg gatcgttcgg
     1261 tagccggcga gcacggcggc gtgcttggcg tcgtcgacgg tgcgggtgag tgggagacgg
     1321 tcgatgtcgc cggttgcggc gtggtgtacc gctggcatcc agcggccgcc gggcccgggg
     1381 atcacgtaga cggtggtgcg ctcgtcgagc cggcggagtt cagcggatgt cgatccttcg
     1441 ccgggcatcg gttcccaccc gcggatcgcg ccggccggga tcggtgcggc gacgtcgacc
     1501 cgctgcgggt cgagtgccgg gtaccggcct tgcagggtgt tggtcgcgtc gttgcgggcg
     1561 tcgtcgaggg agttcgcggt tcctgtagcg aggatgccgt caccggtgcg ggctgcgacg
     1621 tcctcgagtc gccacgccgc tgtgtcgccg tgttcgacgg cgacgatgta ggcggtcttg
     1681 ttgagacgca ggtaggcgcc gcgtccggat gcctgttcac cgcgtgactc ccactcggcc
     1741 gcggtgtagg ggccgacgtc gacccactgg ccgacgggca actggtcgag cgaggcagcc
     1801 gggaggcgat ggcggcggtt ggcgggaacg actcggtcgt cgacgatcgt catgcgcggc
     1861 agcggcgacg caaccaggtc gacaccgatc gccacaccga acgcggcggt cagcttgcgt
     1921 gccgggtcga ggacgttctg gagcgtctcc caccggtcgc gtggcttgag ccccgacggg
     1981 tcgaactcgg gcggcgagtc atgtgcggcg aggccgaccc ggtcggtgac catccacgcc
     2041 acctgatttc gcaaggtcgg attgagaccg ggttcggaac cgtgacgggt gaggcgcccg
     2101 gcggcctcgg cgagaatccg atgcggatcc gacgacttcg gcagccgtcc gcgtaccgtc
     2161 gcggtcgcca cgcgtcgtgt cgcg
//
LOCUS       DAORNF010000612         2176 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_37650_length_2176_cov_1.609618, whole genome shotgun sequence.
ACCESSION   DAORNF010000612 DAORNF010000000
VERSION     DAORNF010000612.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37650_length_2176_cov_1.609618"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            38..>894
                     /locus_tag="PLV68_18760"
     CDS             38..>894
                     /locus_tag="PLV68_18760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC/CoxI family protein"
                     /protein_id="HRE03344.1"
                     /translation="MTETANENGNDTETQQQPTQDGPSLYRRLSARIRASQPVALCTV
                     IDGPHLGAKLLIVPGEPARGSLGDAELDRIAARDGAAELDAGRSGVRHYGPQGQSTPE
                     DLVGSVTVRVFVESFTPPPRMWIFGAVDFTAALASVAKVLGYRVTVCDARDVFATRRR
                     FPMADEVLVSWPGPIFAERGAELGQRDAVCILTHDAKFDVPAVMAALATDVGYIGVMG
                     SRRTHDARMQRLTDAGLTDPAQIARLMSPIGIDIGARTPEETAISICAEIIAVRTGRD
                     APSLRSGTGS"
     assembly_gap    895..944
                     /estimated_length=50
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<945..1369)
                     /locus_tag="PLV68_18765"
     CDS             complement(<945..1369)
                     /locus_tag="PLV68_18765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRE03345.1"
                     /translation="MDDRAGTPNPADGVQAGTIADVARLVGDKWSMLVVRDVFRGVRR
                     FDDLCHDLGVGRAVLADRLKRLVAAGVLVKAPYGERPVRYEYRLTEMGLELSPMLVAL
                     MRWGDRWLGGGEPTAVLVHAPCGTEFEQACVPRLGRQVA"
     gene            1458..2129
                     /gene="cofG"
                     /locus_tag="PLV68_18770"
     CDS             1458..2129
                     /gene="cofG"
                     /locus_tag="PLV68_18770"
                     /EC_number="4.3.1.32"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7,8-didemethyl-8-hydroxy-5-deazariboflavin
                     synthase CofG"
                     /protein_id="HRE03346.1"
                     /translation="MHVTPDTPFDTLLAAVAARPRLTRITYSPKVFIPLTRLCNDSCT
                     YCTFVQPPARLAAPYLTPDEVRRIARSGARAGCHEALFTLGERPEDRYDVARAWLAEH
                     GYTSTVDYLAAMCQMVLDETGLLPHANAGAIGADDLARLRCVSASQGMMLESIADGLD
                     CHRNAPDKVPARRLATLDAAGRLAIPFTTGILVGIGDTRADRLAGLEAIAATATPMKI
                     DTRAP"
BASE COUNT          323 a          812 c          702 g          289 t
ORIGIN      
        1 catctgttca tcgagccgct cgactggtga cagcccgatg accgagacag cgaacgagaa
       61 cgggaacgac accgaaaccc agcagcagcc gacccaggac ggcccgtcgc tgtaccgccg
      121 cctcagcgct cgcatccgcg ccagccagcc ggtggcgttg tgcaccgtga tcgacggtcc
      181 tcacctgggc gccaagctgc tgatcgtgcc gggcgagcca gcgcggggca gcctcggcga
      241 cgccgaactc gaccgcatcg ccgcccgcga cggcgcggcc gaactcgacg cgggccgctc
      301 gggtgtgcgc cactacggcc cgcagggtca gtcgaccccc gaggacctgg tcggatcggt
      361 gaccgtgcgc gtgttcgtgg agagcttcac cccgccgccg cggatgtgga tcttcggcgc
      421 ggtcgacttc accgccgcgt tggcgagcgt ggccaaggtg ctcggctatc gggtcacggt
      481 gtgcgatgcc cgcgacgtgt tcgccacccg ccgccgcttc ccgatggccg acgaggtgct
      541 ggtcagttgg cctggcccga tctttgccga acgcggcgcc gaactcggcc agcgcgatgc
      601 ggtgtgcatc ctgacccacg atgccaagtt cgacgtgccc gccgtgatgg ccgcgctggc
      661 gaccgacgtc ggctacatcg gcgtgatggg cagccgccgc acccacgacg cgcgcatgca
      721 acgcctgacc gatgccgggc tcaccgaccc cgcccagatc gcccggctga tgtcaccgat
      781 cggcatcgac atcggcgcgc gcacgcccga ggagaccgcg atctcgatct gcgccgagat
      841 catcgccgtg cgcaccgggc gcgacgcccc gagcctgcgc tcgggcaccg gctcnnnnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntgcgcg acctgccggc
      961 caaggcgcgg cacgcacgcc tgctcgaact cggtgccgca cggcgcgtgg accaacaccg
     1021 cggtcggctc acccccgccc aaccaccggt caccccagcg catcagcgcc accagcatcg
     1081 gcgacagctc caagcccatc tcggtcaggc ggtattcgta gcgcaccggc cgctcgccgt
     1141 agggagcctt gacgagcacg cccgcggcca ccaaccgctt gagccgatcg gccagcaccg
     1201 cccggcccac gccgaggtcg tggcacagat cgtcgaagcg gcgcacgccg cggaacacgt
     1261 cacgcaccac caacatcgac cacttgtcgc cgaccaggcg ggcgacgtcg gcgatcgtgc
     1321 ctgcctgcac gccgtccgcg ggattcggtg tgccggctcg gtcgtccact cgggcgacgc
     1381 taccggtggc gacacctgtc gagttcgccg tcctgagttg ggttcggaat gcgaactcga
     1441 cttgctatcg tgcccgcatg cacgtcacgc ccgacacgcc cttcgacacg ctgctggccg
     1501 cggtcgcggc acgtcctcgc ctgacgcgca tcacctactc acccaaggtc ttcatcccgc
     1561 tgacgcgcct gtgcaacgac tcgtgcacct actgcacctt cgtccaaccc cccgcccgcc
     1621 tggctgcgcc gtacctgacg cccgacgagg tgcgccgcat cgcccgcagc ggggcgcggg
     1681 cggggtgcca cgaggcgctg ttcacgctgg gcgagcgacc cgaggaccgc tacgacgtcg
     1741 ctcgggcatg gctggccgag cacggctaca cctcgaccgt cgattacctg gcggcgatgt
     1801 gccagatggt gctcgacgaa accggcctgc tcccccacgc caacgcgggc gcgatcggcg
     1861 ccgacgactt ggcccgcctg cgctgcgtgt cggcgagcca ggggatgatg ctcgagtcga
     1921 tcgcggacgg cctcgactgc caccgcaatg cgccggacaa ggtgccggcg cggcggctgg
     1981 ccacgttgga cgcggccggg cggctggcga tcccgttcac caccggcatc ctcgtcggca
     2041 tcggcgacac ccgcgccgac cgtttggccg ggctggaggc gatcgcggcg acggcgacgc
     2101 cgatgaagat cgacacccgc gcgccataga cgacgcgcga aaacacgtcg cggccgagcg
     2161 tgtcagtgcc catgta
//
LOCUS       DAORNF010000613         2153 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_38157_length_2153_cov_1.402765, whole genome shotgun sequence.
ACCESSION   DAORNF010000613 DAORNF010000000
VERSION     DAORNF010000613.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38157_length_2153_cov_1.402765"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1054
                     /locus_tag="PLV68_18775"
     CDS             <1..1054
                     /locus_tag="PLV68_18775"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRE03347.1"
                     /translation="YRAAAMASHEHIAVVEVDEADCDLTLFGDRTLDLWPQPQPSDRA
                     AVMFTSGTTGRPKGVEITQANYAFVGATMASAAGLTARSRQLVVLPMFHANAQYYSVS
                     SAIWEGASVALMPTFSATRFLAQAATHGATHASLFAAPIRMILSRGATPVEGFALQHC
                     WYAMNISDDQYEALAGLLGCRPRQLYGMTETIAAVLTDEAEHPMASSMGYVTPRCIVR
                     IQRSDGAEAGLEEVGEIVVGGVPGITLFAGYLDDPETTAASFRDGWFLTGDRARRVET
                     GRFFFDGRRADVLKVSGENVSTVEVEQVLAAHPAVLEAAVVGMPDPVRDEVPDLPLTP
                     ISICWAISGRICGLPR"
     gene            1102..1263
                     /locus_tag="PLV68_18780"
     CDS             1102..1263
                     /locus_tag="PLV68_18780"
                     /inference="COORDINATES: protein motif:HMM:NF024591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03348.1"
                     /translation="MHAWCEARLTKAKRPRDITLVDELPRTSVGKIRKFLLKDPNAES
                     VPQSPGVPA"
     assembly_gap    1368..1452
                     /estimated_length=85
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1453..1757)
                     /locus_tag="PLV68_18785"
     CDS             complement(<1453..1757)
                     /locus_tag="PLV68_18785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03349.1"
                     /translation="MARVGASGVEVDEHEALPDLDRQRRQRELLLVETLGALHRRGTQ
                     QAAGEVVAPQVVEAGELGAVAVTFHQLHAAVLADRGERTDRLVLGAGDQNRLAVDGGG
                     "
     gene            1798..2145
                     /locus_tag="PLV68_18790"
     CDS             1798..2145
                     /locus_tag="PLV68_18790"
                     /inference="COORDINATES: protein motif:HMM:NF013044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HRE03350.1"
                     /translation="MCPGTFILEDLPWNWKVMFENFNDGYHANKLHHTIQDFCPSELA
                     AFPVEWTEQSNVIFRTNGYVHIDAGFNATLKALMPIFPKLTDEERWRSTFALMPPSLC
                     FGTAPEYHRYSGR"
BASE COUNT          347 a          734 c          646 g          341 t
ORIGIN      
        1 ctatcgcgcc gccgccatgg ccagccacga acacatcgcg gtggtcgaag tcgacgaggc
       61 cgactgcgac ctgaccctgt tcggcgaccg cacgctcgac ctctggccgc agccgcagcc
      121 cagcgatcgg gccgcggtga tgttcacctc gggcaccacc gggcggccca agggcgtcga
      181 aatcacgcag gccaactacg cgttcgtcgg ggccacgatg gcgtcggcgg cgggtctgac
      241 cgcccgctcg cgccaactcg tcgtgctgcc gatgttccac gccaacgcgc agtactacag
      301 cgtgtcgtcg gcgatctggg agggcgcgtc ggtggcgttg atgccgacgt tctcggcaac
      361 gcgcttcctg gcccaggcag cgacccacgg cgccacccac gccagtctgt tcgcggcgcc
      421 gatccgcatg atcctcagcc gcggcgcaac ccccgtcgag gggttcgcgc tgcagcactg
      481 ctggtatgcg atgaacatca gcgacgacca gtacgaggcc ttggccggtt tgctcggttg
      541 ccgcccccgc cagttgtacg gcatgaccga gaccattgcc gccgtgctca ccgacgaggc
      601 cgagcacccg atggcgtcct cgatgggcta cgtcacgccc cgctgcatcg tgcgcatcca
      661 gcgcagcgac ggtgccgaag ccgggttgga agaggtcggc gagatcgtgg tcggtggggt
      721 accgggcatc acgctgttcg ccggatacct cgacgacccc gagaccacag cagcctcgtt
      781 ccgcgacggt tggtttctca ccggtgaccg tgcccgacgg gtggagaccg ggcggttctt
      841 cttcgacggc cgccgcgccg acgtgttgaa ggtgtccggc gagaacgtgt cgaccgtcga
      901 ggtcgagcag gtgctcgccg cgcatcccgc ggtgctggaa gcggccgtgg tcggcatgcc
      961 cgacccggtg cgcgacgagg tgccggactt gcccttgacg ccgatctcga tctgctgggc
     1021 gatctcgggc cggatctgcg ggttgcccag gtagaagtag ccgtcgccgc gcacgtcgcc
     1081 gagccaccga ccgctgccga actgcatgcg tggtgcgagg cccggctcac caaggccaag
     1141 cggccgcgtg acatcacgct ggtcgacgaa ctgccccgca ccagcgtggg caagatccgc
     1201 aagtttctgc tgaaagaccc gaacgccgag agcgtcccac aatcacctgg agtacccgca
     1261 tgaccgtgct caccgacgaa ctgctgcagt ccttcgacgc gtcgaccacg cactacacca
     1321 cggcgctcac gatgccgccc gtgatctaca cctcgcccga gttcctcnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnccaccacc gtcaacggcg agccgattct ggtcacccgc gccaaggacg
     1501 agtcgatccg tgcgttctcc tcgatctgcc agcaccgcgg catgcagctg gtggaaggtg
     1561 acggcaactg caccaagttc acctgcctct accacctgtg gagctacgac ctcaccggcc
     1621 gcctgttggg tgccccggcg atggagcgca ccgagggttt cgacaagaag gagttcccgc
     1681 tgccgccgtt ggcggtcgag atctggaagg gcttcgtgtt cgtcaacttc gaccccgctc
     1741 gcgccgaccc tggccaccta cgagccgtac gtcgaaaact acgatctcga caccgcggtg
     1801 tgcccaggca cgttcatcct cgaagacctg ccgtggaact ggaaggtcat gttcgagaac
     1861 ttcaacgacg gctaccacgc caacaagctg caccacacca ttcaggactt ctgccccagc
     1921 gagctggccg cgttccccgt cgagtggacc gagcagtcga acgtcatctt ccgcaccaac
     1981 ggctacgtcc acatcgacgc cgggttcaac gccacgctca aggcgctgat gccgatcttc
     2041 cccaagctga ccgacgagga gcggtggcgc tcgacgttcg cgctgatgcc gccgtcgctg
     2101 tgcttcggca ccgcgcccga atatcatcgt tattctggcc gatgatctgg gct
//
LOCUS       DAORNF010000614         2147 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_38292_length_2147_cov_1.547801, whole genome shotgun sequence.
ACCESSION   DAORNF010000614 DAORNF010000000
VERSION     DAORNF010000614.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38292_length_2147_cov_1.547801"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..698
                     /locus_tag="PLV68_18795"
     CDS             <1..698
                     /locus_tag="PLV68_18795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HRE03351.1"
                     /translation="GGVHAGRIKDIEHGIALIAKLHALILRREETAAPEAIIERLVAA
                     IQASKDRPLPKLLTALGIKHLGPSAAESLSREFGTLDAIMQAGEADLASVDGLGGIIA
                     RSILRWFSQPANQEFVEKLRAAGVEFGKVVVSRLAQNLAGKAIVVTGTLQGYNREEAE
                     AAIKDRGGKSPGSVSKKTFAVVVGADPGASKLTKATELGIPVLDDAGFAHLLAHGELP
                     ATSDAPDGGPPDN"
     gene            complement(695..1117)
                     /locus_tag="PLV68_18800"
     CDS             complement(695..1117)
                     /locus_tag="PLV68_18800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03352.1"
                     /translation="MASSWLSSGCSSVSGGGGQPLPEAIQSVLPVRNATQVQQQESIF
                     VDLAEGYTGTVTINGVVLETVNLSDLSQNPTDSGAQVKLPPTVIFEPGNFTLTFTPTD
                     GALVERFLTGVNVVSLVYWKIVDGPNFARTFTWQFDVI"
     gene            1317..>1763
                     /locus_tag="PLV68_18805"
     CDS             1317..>1763
                     /locus_tag="PLV68_18805"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HRE03353.1"
                     /translation="MASTDSLIGQLVADRYRLLERVGAGAAGAVFVGVDEQGDQQADR
                     PSGRSVVVKLLRPQYTATAETEARFRLEAQIAASLAQPNLNAVYDFGVDDVAGTRLAF
                     VVSEPLGGASLRDMLDRGRLLSPSQALMVGLDVCRGLDAMHRAGVIH"
     assembly_gap    1764..1840
                     /estimated_length=77
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1841..>2147
                     /locus_tag="PLV68_18810"
     CDS             <1841..>2147
                     /locus_tag="PLV68_18810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03354.1"
                     /translation="QMWDDPAGVGLDVARYASPEQARGATLRDGGGLTAATDIYSLAL
                     VLVEAVTGQVPFTADSTVATLNARQDKLLPVSADFGPLASVLSRAANPSADDRYSAA"
BASE COUNT          331 a          690 c          717 g          332 t
ORIGIN      
        1 atggcggggt ccacgccggg cggattaaag atatcgagca cgggatcgcc ctgattgcga
       61 aacttcacgc cctgatactt cgccgggagg aaaccgctgc cccagaggcg atcatagagc
      121 ggctggtcgc cgcgatccag gcgtcgaagg accggccgct tcccaagctg ttgacggccc
      181 tcggcatcaa gcacctcggc ccgtccgcgg ccgagtcact cagccgtgag ttcggcaccc
      241 tcgacgcgat catgcaggcc ggcgaggccg atctggcgtc ggtcgacggc ctcggtggca
      301 tcatcgcccg ttcgatcctg cgctggttct cccagccggc caaccaggag ttcgtcgaga
      361 agctgcgcgc cgcgggcgtc gagttcggca aggtggtcgt cagccgcctg gcgcagaacc
      421 tggccggcaa ggccatcgtg gtcactggaa cattgcaggg ctacaaccgc gaggaagccg
      481 aggcggccat caaggatcgc ggtggcaaga gcccgggcag cgtcagcaag aagacgttcg
      541 ccgtcgtcgt cggtgccgac cccggcgcct ccaagctcac caaggcgacc gagttgggca
      601 tcccggtgct cgacgacgcc gggttcgccc acctgctcgc ccacggcgag ctgccagcaa
      661 cgtccgacgc ccccgacggg ggcccgccag ataactagat gacgtcgaac tgccaggtga
      721 aggtgcgggc gaagttgggt ccgtcgacga tcttccagta cacgagcgac acgacgttga
      781 ccccggtgag gaagcgctcg accagtgccc cgtcggtggg ggtgaaggtc aatgtgaagt
      841 tgccgggttc gaagatgacc gtggggggca gcttgacctg cgctcccgag tcggtcgggt
      901 tctgtgacag gtcggacagg ttgacggtct ccagcacgac gccgttgatc gtgaccgtgc
      961 cggtgtagcc ctccgccagg tcgacgaaga tcgattcctg ctgttgcacc tgggtggcgt
     1021 tgcgaaccgg cagcacgctt tggatcgcct cgggcaacgg ctggccgccg ccacctgaga
     1081 ccgacgagca ccccgacgac aaccaggatg atgccaagtg cgatcagcat gctgacgatg
     1141 gcgcgggcca tgacgggtgg cacggcaggg atacgtcgct tcacagcgcc agtcttgtcg
     1201 accggactcg gaatcacacg ttcggcacca cgggttcgcc accacacctc cggctggctg
     1261 gtgcggggcc gggcgcgacc gctcgattgc gacccggcgg tagtttcaga gtcctcatgg
     1321 caagcacgga ctcgctcatc ggacagctcg tcgccgaccg ctatcgcctg ctcgaacggg
     1381 tgggtgctgg tgctgctggc gcggtgttcg tgggtgtcga cgagcagggt gaccagcagg
     1441 ccgatcgccc cagtggtcgg tcggtcgtgg tcaagttgct gcgaccgcag tacacggcca
     1501 ccgccgagac cgaggcgcgc ttccggctcg aggcccagat cgcggcatcg ctggcgcaac
     1561 ccaacctcaa cgcggtgtac gacttcggcg ttgacgatgt cgccggcacg cggctggcct
     1621 tcgtcgtgtc cgaaccgctc gggggcgcca gtttgcgcga catgctcgac cgcggccggc
     1681 tgctcagccc gtcgcaggcg ttgatggtgg ggctcgacgt gtgccgcggc ctcgatgcga
     1741 tgcatcgcgc aggtgtgatc cacnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn agcagatgtg ggacgacccc
     1861 gcgggcgtcg gtctcgacgt ggcccgctac gcctcgcccg aacaggctcg cggggccacc
     1921 ttgcgcgacg gtggcgggct gacggccgcg accgacatct actcgctggc gctggtgctg
     1981 gtcgaggccg tcacggggca ggtgccgttc acggctgact ccaccgtcgc caccctcaac
     2041 gctcgtcagg acaagctgct gccggtatcg gccgacttcg gtccgctggc ctcggtgctg
     2101 tcgcgggcgg ccaacccgtc ggccgacgac cgctacagcg ctgccga
//
LOCUS       DAORNF010000615         2145 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_38331_length_2145_cov_1.589474, whole genome shotgun sequence.
ACCESSION   DAORNF010000615 DAORNF010000000
VERSION     DAORNF010000615.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38331_length_2145_cov_1.589474"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..841)
                     /gene="mreB"
                     /locus_tag="PLV68_18815"
     CDS             complement(<1..841)
                     /gene="mreB"
                     /locus_tag="PLV68_18815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018225978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HRE03355.1"
                     /translation="MARDLAIDLGTANTLVYMKGRGIVLNEPSVIALNRQTGEVLATG
                     REAWQMIGRTPGYIVAVRPLRGGAITDFEITERMIRLLLQRVGVSRFTRPKVVICVPS
                     AITEVERRAVTDAARRAGAADAQLIEQPMAAAIGADLPIDEPVGNMVIDIGGGTSETA
                     VISLGGVVALEAVRVGSFDIDAAIQNYVRREHGIAIGERTAEEIKVAIGSAEPVGDSM
                     VMSFRHLDAVYKIDRSSGEIIWKLGGTPIDEQLEVLNDPHGAFPFGGQHDAPRVVHQE
                     EDLQ"
     gene            complement(844..1029)
                     /locus_tag="PLV68_18820"
     CDS             complement(844..1029)
                     /locus_tag="PLV68_18820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03356.1"
                     /translation="MILIIVGVLVLGALIVMVVNRRRTPTDGVASFRRHIDALSPEAR
                     REVIDRVQNARESGKGK"
     gene            complement(1035..1577)
                     /locus_tag="PLV68_18825"
     CDS             complement(1035..1577)
                     /locus_tag="PLV68_18825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HRE03357.1"
                     /translation="MFNPPDSDTWVAVGTDPLPVAAAYEWAVRPHCGAVVLFSGTVRD
                     HAEGRSGVQHLTYEAYDEQVVAALGAVADEARTRWPSVGAIALLHRLGRIELEESSVV
                     VVVSAPHRGEAFEAGRYAIDATKVSAPIWKHEVWDDGAGWGTGAADLCDPADVGSAVG
                     SPSTGSPSVTSPSIGVGAHR"
     assembly_gap    1855..1913
                     /estimated_length=59
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1914..>2145
                     /locus_tag="PLV68_18830"
     CDS             <1914..>2145
                     /locus_tag="PLV68_18830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03358.1"
                     /translation="GLLWMIVPHGRHDGAAEEAGANGAVAIAAEVSGSASIGAGGVGG
                     VGGVGGHDDSDDAGDQLGSGFVANSFPLETAGA"
BASE COUNT          322 a          741 c          691 g          332 t
ORIGIN      
        1 gctggagatc ttcctcttga tggacgaccc gtggcgcatc gtgctgacca ccgaagggga
       61 atgctccgtg gggatcgttc aggacctcga gctgctcgtc aatgggcgtg ccaccgagct
      121 tccagatgat ctctccgctg gaacggtcga tcttgtagac cgcatcgagg tgccggaacg
      181 acatgaccat cgagtcaccg accggctcgg ccgagccgat cgcgaccttg atctcttccg
      241 cggtgcgctc gccgatcgcg atgccgtgct cgcggcgcac atagttctgg atcgcggcgt
      301 cgatgtcgaa gctgccgacc cgcaccgctt ccaacgccac caccccaccg aggctgatca
      361 cggcggtctc gctggtgccg ccgccgatgt cgatcaccat gtttccgacc ggttcgtcga
      421 tcggcagatc ggcaccgatc gcggcagcca tcggttgctc gatcagctgg gcgtcggccg
      481 cgcccgcgcg ccgagcagcg tcggtgaccg cgcggcgctc gacttcggtg atcgccgacg
      541 gtacgcagat gacgaccttg ggccgggtga agcggctgac gccgacgcgt tgcagcaaca
      601 gccggatcat ccgctcggtg atctcgaagt cggtgatcgc gccgccgcgc aacgggcgca
      661 cggcgacgat gtagcccggc gtgcgtccga tcatctgcca cgcctcgcga ccggtggcca
      721 agacctcgcc ggtctggcgg ttgagggcga tgaccgacgg ctcgttcaac acgatgccgc
      781 ggcctttcat gtacaccagc gtgttggccg tgccgaggtc gattgccagg tcgcgcgcca
      841 cgtctacttc cctttgcccg actcgcgggc gttctgtacg cggtcgatca cctcgcggcg
      901 cgcctcgggc gagagcgcgt cgatgtgacg ccgaaagctg gccactccgt cggtcggcgt
      961 gcggcgacgg ttgacgacca tgacgatcag cgcgcccagc acgagcacgc cgacgatgat
     1021 caggatcacg cccgtcatcg gtgcgccccg acaccgatcg aagggctggt gaccgaaggg
     1081 ctgccagtcg acggactgcc gaccgcactg ccgacgtcgg cggggtcgca caggtcggct
     1141 gcgcccgtac cccagccggc gccgtcgtcc cacacttcgt gcttccagat cggtgcgctg
     1201 accttggtcg cgtcgatcgc atagcgcccg gcttcgaacg cttcgccacg atggggtgcc
     1261 gacacgacga ctaccaccga cgactcttcc agctcgatcc gccccagccg atgcagcaat
     1321 gcgatcgcac cgaccgacgg ccagcgggtg cgggcctcgt ccgcgaccgc accgagcgcg
     1381 gccacgacct gctcgtcgta ggcctcgtag gtgaggtgct gcacgcccga gcgcccctcg
     1441 gcgtggtcgc gcaccgtgcc cgagaacaac accaccgcgc cacagtgcgg acgcaccgcc
     1501 cattcgtagg cggcggcaac gggtaggggg tcggtgccga ccgccaccca tgtgtcgctg
     1561 tcgggcgggt tgaacatcgc tcgcatcgta gccgtgacca ctgagaacac ccctcaacga
     1621 cctggcagca ccaccagcga cacccgaccg ttcgctcggg ccgctcatcg gccgcgcggc
     1681 tggccgcgat gacactccgt ggccgcccct ggctaccatc tcctcttcgt ggaaggcaga
     1741 gacgggtcga acgactggga cgacgaggcg tcgtacccaa ccgccccgtt gccaccccac
     1801 gaacgggcct ggcgccatcc ctccgaagtt ggtgccgagc agtggatcgc caccnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnggcctgc
     1921 tgtggatgat cgtgcctcac ggtcgtcacg acggcgcggc cgaggaggcg ggcgccaacg
     1981 gtgccgtcgc gatcgccgcc gaggtgtctg gctcggcatc gatcggtgcc ggcggcgtcg
     2041 gtggcgtcgg tggcgtcggt ggccacgatg acagcgacga cgctggcgat cagctcggca
     2101 gtgggtttgt cgccaactcg ttcccactcg aaaccgccgg ggcga
//
LOCUS       DAORNF010000616         2134 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_38577_length_2134_cov_1.940837, whole genome shotgun sequence.
ACCESSION   DAORNF010000616 DAORNF010000000
VERSION     DAORNF010000616.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38577_length_2134_cov_1.940837"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>210
                     /locus_tag="PLV68_18835"
     CDS             <1..>210
                     /locus_tag="PLV68_18835"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRE03359.1"
                     /translation="ATDRSADAEPKEAIDRAFRPDITVVDGDVIARLRGLTLRAVHTP
                     GHTSNHLCVGIDETRALFSGDHVMG"
     assembly_gap    211..247
                     /estimated_length=37
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <248..573
                     /locus_tag="PLV68_18840"
     CDS             <248..573
                     /locus_tag="PLV68_18840"
                     /inference="COORDINATES: protein motif:HMM:NF036805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03360.1"
                     /translation="ESLHKVIGRGDATLYPTHGNPVTDPQPFLAAYLAHRLAREAAVL
                     DLVRAGVGSIRDMVDILYTDVARELHKAARRSVLAHLIKLVADGDVVVADGTRPRAKA
                     SFAAV"
     gene            complement(596..>1116)
                     /locus_tag="PLV68_18845"
     CDS             complement(596..>1116)
                     /locus_tag="PLV68_18845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03361.1"
                     /translation="PFIAPFTGAMSLRQPFNLTLASAINADVRAHIRGVSPEVRTTNQ
                     VLLAQIAASGSSGLFAGQASDCADLLMLAAEQVQSSSPRLIADAISDISSVGSTCATF
                     IACRDLIRAGRGIDFSGSDGLLAIGRDGDVVSARYEQFAFDATGRDVTTQVLIVPSVS
                     FALGDFSDLPGQ"
     assembly_gap    1117..1129
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1135..1875)
                     /locus_tag="PLV68_18850"
     CDS             complement(1135..1875)
                     /locus_tag="PLV68_18850"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRE03362.1"
                     /translation="MAVSASLAGACTTSDDGTTTSSSSSTSTTAVVRVDDGVLRIGVL
                     LPSSGAGASIIGDSARAAVQSAIASANAEGGVLGRDVELVLQDEGADSATAAVGLRAL
                     IDANVDVLIGPASSNIAIALMPSIVDAGLVTCSPGASALALDEFPDDGLFFRTIASDS
                     LQADAMATVLERTGERSAVIAYVDDGYGRPFEEALRFQLRRRGIAVDASVGYAADDND
                     YGAEAARLVTSGDGAIALIGGGRHAVRR"
BASE COUNT          345 a          757 c          694 g          288 t
ORIGIN      
        1 ccgcgaccga cagatcggcc gacgccgagc cgaaggaggc gatcgaccgc gcctttcggc
       61 ccgacatcac cgtggtcgac ggcgacgtca tcgcccgcct gcgcgggctg acattgcgcg
      121 ccgtgcacac gcccggtcac acgtcgaacc acctgtgcgt cggcatcgac gagacacgcg
      181 ccctgttcag cggcgatcac gtcatgggct nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnntcg agtcgctgca caaggtgatc gggcgtggcg acgcgaccct ctaccccacc
      301 cacggcaacc cggtcaccga cccccagccg ttcctggccg catatctggc ccatcgcctg
      361 gcccgcgagg ccgcggtgct cgatctcgtc cgggccggtg tcggctcgat ccgcgacatg
      421 gtcgacatcc tctacaccga cgtcgcgcgc gagttgcaca aggccgcccg gcgcagcgtg
      481 ctcgcccacc tgatcaagct ggtggccgac ggcgatgtgg tcgtcgccga cggcacccgg
      541 ccacgcgcga aggcctcctt cgcggccgtg tgacccgccc agtgacggga ccgaatcact
      601 ggcctggcag atcgctgaag tcaccgaggg caaagctgac cgacggcacg atcagcacct
      661 gggtggtgac gtcacgacct gtcgcgtcga aggcgaactg ttcgtaacgg gccgacacca
      721 cgtcaccgtc acggccgatg gcgagcaggc catcgctgcc actgaagtcg atgccgcgac
      781 cggcacggat caggtcgcga cacgcgatga acgtcgcaca cgtcgaaccg accgagctga
      841 tgtccgagat cgcgtccgcg atcaggcgcg gcgaactcga ctgcacctgt tcggccgcga
      901 gcatcaacag gtcggcgcag tcggacgcct gccctgcgaa caggccgctc gatccactcg
      961 ccgcgatctg ggccagcaac acctggttgg tcgtgcgcac ctcgggtgac accccgcgga
     1021 tgtgagcccg cacgtcagcg ttgatcgcac tcgccagggt gaggttgaac ggctggcgca
     1081 gcgacatggc gccggtgaac ggggcgatga acggcannnn nnnnnnnnng cgggtcagcg
     1141 gcggacggca tggcgtcctc ctccgatcag ggcgatcgcg ccgtcgccgc tggtgaccaa
     1201 gcgagccgcc tcggcgccat agtcgttgtc gtcggccgcg tatccgaccg aggcgtcgac
     1261 ggcgatccct cgccgccgca actggaagcg cagcgcctct tcgaacgggc ggccgtaacc
     1321 gtcgtcgacg taggcgatca ccgccgagcg ctcacccgtg cgttccaaca ccgtcgccat
     1381 cgcgtctgcc tgcaagctgt cggaggcgat ggtgcggaaa aacagcccgt cgtcggggaa
     1441 ctcgtccagc gccaatgccg aagcccccgg cgagcaggtg acgagaccgg catcgacgat
     1501 cgacggcatc agcgcgatcg cgatgttgga cgacgccgga ccgatcagca catcgacgtt
     1561 ggcgtcgatc aacgcgcgca accccacggc cgcggtcgca gagtcggcgc cttcgtcctg
     1621 cagcaccagt tccacgtcgc gcccgagcac accgccttca gcgttggccg acgcgatggc
     1681 cgactgcacc gcagcacgcg ccgaatcgcc gatgatcgag gcaccggcac cgctcgacgg
     1741 cagcagcacg ccgatgcgca gcaccccgtc gtcgacccgc accacggcgg tggtcgaggt
     1801 ggacgacgaa ctcgacgtgg tggtgccgtc gtcgctggtg gtgcacgccc cggccaggct
     1861 tgcgctcacc gccacggcgg cacacgcggc gcgtacacga cgaggggcca tggcggccga
     1921 gtgtaggagt tcaggcgccg accggctgtt ctgccagcca cctctgcagc cgcgtcggcc
     1981 atgacggcct gttcgggccg ggcctgttct ggcaacatca tcggtgtgga cccgtacgtg
     2041 caacagctgc tcgacgccct ggagccggcg gtcggcccgt ggctggagcg ctgcgtcgtc
     2101 accaccgcag cggcgcagac cggcaccgcc agcg
//
LOCUS       DAORNF010000617         2132 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_38624_length_2132_cov_1.597496, whole genome shotgun sequence.
ACCESSION   DAORNF010000617 DAORNF010000000
VERSION     DAORNF010000617.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38624_length_2132_cov_1.597496"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="PLV68_18855"
     CDS             <1..198
                     /locus_tag="PLV68_18855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HRE03363.1"
                     /translation="ERLDELVAGNDEHVAMVRQLEALYDATPDAANPPLDVTPMAEGE
                     LPTADELGAEFERFLRDQNNG"
     assembly_gap    223..374
                     /estimated_length=152
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <375..1316
                     /locus_tag="PLV68_18860"
     CDS             <375..1316
                     /locus_tag="PLV68_18860"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HRE03364.1"
                     /translation="SGAWTAETAHDPFFPHVLAAEHTSTIELGTSIAVAFARNPMTLA
                     NIGWDLQTYSKGRFILGLGSQIKPHITKRFSMEWSHPAPRMREMIMAIRAIWDTWLNG
                     TPLAFRGDFYQHTLMTPFFTPNPADLGDFGVPKIFLAGVGELMTEVAGEVCDGFICHG
                     FTTERYLREVTLPALQRGRAKAGKTMEGFEIVGPSFVVTGNTEDELAKAVAGTRQQIA
                     FYGSTPAYRPVLDMHGWGELQSELNALSKLGKWVEMGDLISDEVLNTFAVVGEPEAIA
                     PELHTRYGDVIERISIYAPYHSDPERWRKVFEALKSA"
     assembly_gap    1371..1390
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1627..>2132)
                     /locus_tag="PLV68_18865"
     CDS             complement(1627..>2132)
                     /locus_tag="PLV68_18865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRE03365.1"
                     /translation="LPERHVGELLIRGTSVTPGYYKRPDATAALFYDDWLCTGDLGYL
                     LDGELVLCGRIKDVIIVGGRNVFPEDIERAVGGLEGVRAGNVIAFGVEGYKGKETVVV
                     VAEVRADDLAAVREAIHHRTIEVCGLPPRDVMLVKPGTLPKTSSGKLQRAKCRDLYLE
                     ESLDLLT"
BASE COUNT          363 a          677 c          620 g          300 t
ORIGIN      
        1 gaacggctcg acgaactggt cgccggcaac gacgagcacg tcgcgatggt gcgccaactc
       61 gaagcgttgt acgacgcgac accagacgcc gcgaaccccc cactcgatgt cacaccgatg
      121 gccgagggcg agctccccac ggccgacgag ctcggtgccg agttcgaacg gttcctgcgc
      181 gaccagaaca acggttgatc ctcgctggcg ccgagctccg gcnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnagcggg gcgtggacgg cagagaccgc ccacgatccc ttcttccccc
      421 acgtgctggc cgccgagcac acctccacca tcgagctcgg tacgtcgatc gccgtcgcgt
      481 tcgcccgcaa cccgatgacg ttggccaaca tcggttggga cctgcagacc tacagcaagg
      541 gccggttcat cctcggtctc ggcagccaga tcaaaccgca catcaccaag cgcttctcca
      601 tggagtggag ccatcccgcg ccgcgcatgc gagagatgat catggccatc cgcgccatct
      661 gggacacgtg gctcaacggg acaccgctcg cgttccgggg cgacttctac cagcacacgc
      721 tgatgacccc gttcttcacg ccgaaccccg ccgatctcgg cgacttcggc gtaccgaaga
      781 tcttcctcgc cggtgttggt gagttgatga ccgaagtcgc aggcgaggtc tgcgacgggt
      841 tcatctgcca cgggttcacc accgagcggt acctgcgcga ggtcaccctg ccggcgctgc
      901 agcggggccg ggccaaggct ggcaagacga tggagggctt cgagatcgtc gggccgagct
      961 tcgtggtcac cggcaacacc gaggacgagt tggccaaggc tgtcgcgggc acgcgccagc
     1021 agatcgcctt ctacggctcg acgcccgcgt accgcccggt gctcgacatg cacgggtggg
     1081 gcgagctgca gagcgagctc aacgcgctga gcaagctggg caagtgggtc gagatgggcg
     1141 acctgatcag cgacgaggtg ctcaacacct ttgctgtcgt cggcgaaccc gaggcgatcg
     1201 cccccgaact ccacacccgc tacggcgacg tgatcgaacg catcagcatc tacgccccct
     1261 accacagcga cccagagcgc tggcgcaagg tcttcgaagc cctcaaatcc gcctgaccct
     1321 caccccaccc cacacaaccg cgccacctcc ggcaacagcg gatacgggtc nnnnnnnnnn
     1381 nnnnnnnnnn gatgaagcga taggtccgca cgcccgccag gcggacgatc tctccaagca
     1441 aactccgtca aggcatttcc aatcacggta agaggttatg gaagtcggta ccgagagtcg
     1501 tggcatgccc tagggctcac tcggcacccc actgatgtgc atccatgatg cttcgcgaac
     1561 gacgcgccgc cgcccgccaa cgccagcggg tcagtcgagc caggacgtcc ggcccacctc
     1621 gaacgtctac gtgaggaggt cgagggattc ttcgaggtag aggtcgcggc acttggcgcg
     1681 ttggagtttg ccggagctgg tcttggggag ggtgccaggc ttgaccagca tcacgtcgcg
     1741 cgggggaagg ccgcagacct caatggtgcg gtggtggatc gcttcgcgca ccgcggcgag
     1801 atcgtcggcg cgcacttcgg ccaccacgac gacggtctcc ttgcccttgt agccctcgac
     1861 accgaacgcg atcacgttgc cggcgcggac accctcgagg ccaccgacgg cccgctcgat
     1921 gtcttcgggg aacacgttgc ggccgccgac gatgatcacg tccttgatcc gcccgcacag
     1981 caccagttca ccgtcgagca gatacccgag gtcaccggtg cacagccagt cgtcgtagaa
     2041 cagggccgct gtcgcatcgg ggcgcttgta gtagccgggg gtcaccgagg tgccgcggat
     2101 gagcagctcg ccgacatggc gctcgggcag gg
//
LOCUS       DAORNF010000618         2131 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_38654_length_2131_cov_1.257225, whole genome shotgun sequence.
ACCESSION   DAORNF010000618 DAORNF010000000
VERSION     DAORNF010000618.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38654_length_2131_cov_1.257225"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>220)
                     /locus_tag="PLV68_18870"
     CDS             complement(<1..>220)
                     /locus_tag="PLV68_18870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRE03366.1"
                     /translation="TGRPVRIAYSREESFLGHVHRHPATIWMRHHADADGRIVRIDAR
                     FVFDGGAYASTSAAVLINGITHTQGPYKC"
     assembly_gap    221..290
                     /estimated_length=70
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<291..>667)
                     /locus_tag="PLV68_18875"
     CDS             complement(<291..>667)
                     /locus_tag="PLV68_18875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRE03367.1"
                     /translation="ILAVPADHPEPCRRALAAIQVTYEVLTPLVDPERAIDGSFPPIH
                     PDGNLIRHQRIVCGDPEATGPIVVEGRYEIGMQDQAFLGLEAALALPDPGGAGVELYV
                     ATQWLHEDRKQIAACLGLADDKV"
     assembly_gap    668..677
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<678..1030)
                     /locus_tag="PLV68_18880"
     CDS             complement(<678..1030)
                     /locus_tag="PLV68_18880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03368.1"
                     /translation="MSITEAPRRHDRDAGRSNGRLGDSITRPDGTAKVQGSFAFSSDL
                     HAENCLWGATLRSPHPHARIVAIDTTPAWKIAGVEAIVTADDVPGKMTYGLISQDQPV
                     FARDVLHVGAEGFGLL"
     gene            complement(1027..1293)
                     /locus_tag="PLV68_18885"
     CDS             complement(1027..1293)
                     /locus_tag="PLV68_18885"
                     /inference="COORDINATES: protein motif:HMM:NF013923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HRE03369.1"
                     /translation="MGVLGRVLRVHLGGHLVEAGAVQCGVCTPGLIMATHDLLERHPV
                     PSEMQIREELSGNVCRCTGYGRIIDAVQRVAVARRDQADAGAVS"
     assembly_gap    1310..1474
                     /estimated_length=165
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1553..>1789)
                     /locus_tag="PLV68_18890"
     CDS             complement(1553..>1789)
                     /locus_tag="PLV68_18890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03370.1"
                     /translation="TIVRCHEAESAADAAIDWDASPDDGLGSGLDDAFAERFAGLAAA
                     ASRPIDDHRATAAYRRHAVGVMAARLLRRAFPHD"
     assembly_gap    1790..1862
                     /estimated_length=73
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1863..2098)
                     /locus_tag="PLV68_18895"
     CDS             complement(<1863..2098)
                     /locus_tag="PLV68_18895"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HRE03371.1"
                     /translation="MGGNLGTCSPAGDGLPVLSALDAQVHLASAEGERQMPIGEFMVG
                     VKRNARRPGELITAVTVPVLDGFQGYSKVGVRNA"
BASE COUNT          264 a          623 c          650 g          276 t
ORIGIN      
        1 gcacttgtac gggccctgag tgtgggtgat gccgttgatc agcacggcgg ccgaggtcga
       61 cgcataggca ccgccgtcga acacgaaccg agcgtcgatg cgcacgatcc gcccgtcggc
      121 gtcggcgtga tgccgcatcc agatcgtggc cggatggcgg tggacgtggc cgaggaagct
      181 ctcttcgcgg ctgtacgcga tgcgcaccgg acggccggtg nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn caccttgtcg
      301 tcggccagcc cgagacaagc cgcgatctgc ttgcgatctt cgtgcagcca ttgcgtcgcg
      361 acgtacagct cgacgccggc gccacccgga tcgggcaggg cgagtgccgc ctccagaccg
      421 aggaaggcct ggtcctgcat gccgatctcg tacctgcctt cgaccacgat cgggccggtc
      481 gcttcgggat cgccacacac gatgcgctga tggcggatca ggttgccatc gggatggatc
      541 ggtgggaacg agccgtcgat cgcccgctcg gggtcgacca gcggggtcag cacctcgtag
      601 gtgacctgga tcgcggccag ggcacggcgg cacggctcgg ggtgatcggc gggcacggcc
      661 aggatcgnnn nnnnnnnagc aggccgaagc cctcggcgcc gacgtgcagc acgtcgcggg
      721 cgaacaccgg ctggtcctgg ctgatcaacc cgtaggtcat cttgccgggc acgtcgtccg
      781 cagtgacgat cgcttcgacg ccggcgatct tccacgccgg ggtggtgtcg atcgccacga
      841 tgcgggcgtg gggatgcggc gagcgcagcg tggcgcccca caaacagttc tcggcgtgga
      901 gatcggacga gaaggcgaac gatccctgca ccttggccgt gccgtcgggc cgggtgatgc
      961 tgtcgcccag gcgaccgttc gagcgtccgg cgtcgcggtc gtggcgccgc ggggcttcgg
     1021 tgatgctcac gacaccgctc ctgcgtcagc ctggtcgcgg cgggcgacgg ccacccgctg
     1081 caccgcgtcg atgatccgcc cgtagccggt gcagcggcag acgttgccgc tgagctcctc
     1141 gcggatctgc atctcgctgg gcacggggtg gcgttcgagc agatcgtggg tggccatgat
     1201 cagccccggc gtgcagaccc cgcactgcac cgcgcccgcc tcgacgaggt gcccacccag
     1261 gtggacgcgc aggacgcggc ccaggacgcc caagccgtgc tcgctctatn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnacgtcg tggtcgctgc cgttgacatg
     1501 caacgtgtag tgcaggttgc cggactgttg cagcccagac ggctggtgat gctcagtcat
     1561 gaggaaacgc cctccgcagt aggcgtgccg ccatgacccc cacggcgtga cgccggtagg
     1621 cggccgtggc acggtggtcg tcgatcggcc ggctggccgc ggccgccagg cccgcgaacc
     1681 gttcggcgaa ggcgtcgtcg aggccactcc ccaacccatc gtccggcgat gcgtcccagt
     1741 cgatcgccgc gtcggccgcg gactcggcct cgtgacagcg gacgatggtn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnatcgcgtt gcgcaccccc accttgctgt agccctggaa gccgtcgagc acgggcacgg
     1921 tgaccgcggt gatcagctca cccggccgcc gggcgttgcg cttgacaccg accatgaact
     1981 cgccgatcgg catctgccgc tcgccctcgg cgctggcgag atgcacctgc gcgtccaggg
     2041 ccgacagcac gggtaagccg tcgccggccg gcgagcaggt gccaaggttg ccacccagcg
     2101 tgcccgcgtg acggatctgg ggcgagccga c
//
LOCUS       DAORNF010000619         2120 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_38910_length_2120_cov_1.568039, whole genome shotgun sequence.
ACCESSION   DAORNF010000619 DAORNF010000000
VERSION     DAORNF010000619.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38910_length_2120_cov_1.568039"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="PLV68_18900"
     CDS             complement(<1..187)
                     /locus_tag="PLV68_18900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03372.1"
                     /translation="MVVSSTAVVVVVGGSVVVVGAAVVVGTAVVVVVARVVAVSSPQP
                     PQAAVTVTAAANRKESRG"
     gene            272..1147
                     /locus_tag="PLV68_18905"
     CDS             272..1147
                     /locus_tag="PLV68_18905"
                     /inference="COORDINATES: protein motif:HMM:NF022702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HRE03373.1"
                     /translation="MTGVANPDPVRAGRPALVVKIDNVPAARPQTGFNAADIVYEEII
                     NDGYTRFAMVFQSGDANPVGPIRSGRLQDVELFTSYNQPLFAWSGGNRTVTDAINASD
                     LINLGSLTSAGGSAYFRTTNRKAPHNLYATTDALFARTPLWHPPATQQFQYRAVDAAP
                     QGEPSRGVAVKLESINVRWTWDAAQGLYLRQMGGKDHLDAATNSVVTTNNVVVLAVNY
                     VPGISGSPDAQTIGDGEAFVFTGGNYIHAFWHRADKYTPISLVADDGSPVLLQPGRTF
                     IELPRVDNTLPIAAE"
     gene            complement(1067..>2120)
                     /locus_tag="PLV68_18910"
     CDS             complement(1067..>2120)
                     /locus_tag="PLV68_18910"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HRE03374.1"
                     /translation="QVQTSLGGETVFSLNRARARGFQHAAPTVELVRRLLADQHPDLA
                     GQRLTIVANGWDNVIVRVGDDLVARLPRRQAGADLVLNEQRWLPTLATRLPIPIAAPV
                     RFGQPAADYPWHWSICPWFDGEVAADTALADPVGEADRLGAFVRALHQPAPADAPENP
                     FRGQPVGELVPRVHANLARLGLDGAHRAVVAALVDHLAATPEWDGPPVWLHGDLHSAN
                     LIVRDGRIVAVLDLGDLTSGDPAVDLAVAWMLFDGDARARFRSAVDSDDDTWRRARLW
                     AIHFALLYLLHSAGSERFARMGHHLLGAAAAQPESARETGPSTGPARQLLGSDGKGVV
                     DAGQLDEGAAGLKQHG"
BASE COUNT          366 a          793 c          673 g          288 t
ORIGIN      
        1 cgccgcggct ttccttgcgg ttcgcggcag ccgtcacggt gacggcggcc tgcggcggct
       61 gaggcgacga caccgccacc accctggcca ccaccacgac caccgcggtg ccgaccacga
      121 ccgccgcgcc gaccaccacg acgctgccac cgaccaccac gacgacggcc gtcgaagaga
      181 cgaccacgac cacagcggtc ccggaaacga ccacgacggt gtcgcccgga ctaacctgtt
      241 tgtcgtcgac ctgatcgagc cggtctaccc gctgaccggc gtcgccaacc ccgatccggt
      301 ccgcgctggt cgccctgcgt tggtcgtcaa gatcgacaac gtgcccgcgg cgcgccccca
      361 gaccggtttc aacgcggccg acatcgtgta cgaggagatc atcaacgacg gctacacccg
      421 gttcgcgatg gtgttccaga gcggcgatgc caacccggtc gggccgatcc gctccggtcg
      481 cctgcaagac gtcgagctgt tcacgtcgta caaccaaccg ttgttcgcgt ggagcggcgg
      541 caaccgcacc gtcaccgatg cgatcaatgc gagcgacctg atcaacctcg gttcgctgac
      601 ctcggcgggc ggcagcgcct acttccgcac caccaaccgc aaggcgccgc acaacctgta
      661 cgcgacgacc gacgccctgt tcgcccgcac cccgctgtgg cacccaccag cgacacaaca
      721 gttccagtac cgcgctgtcg acgccgcacc ccaaggcgaa cccagccgcg gggtggcggt
      781 caagctcgaa tccatcaacg tccgctggac atgggacgcc gcgcagggcc tgtatctgcg
      841 ccagatgggc ggcaaggatc atctcgacgc ggcgaccaac tcggtggtga ccaccaacaa
      901 cgtcgtcgtg ctcgcggtca actatgtgcc cggcatctcg ggcagccccg acgcccagac
      961 catcggcgat ggtgaggcct tcgtgttcac cggcggcaac tacatccacg ccttctggca
     1021 ccgtgccgac aagtacaccc cgatctcgct ggtcgccgac gacggctcac ccgtgctgct
     1081 tcaacccggc cgcaccttca tcgagctgcc ccgcgtcgac aacacccttc ccatcgctgc
     1141 cgagtagctg tcgggcaggc ccggtggacg gcccggtctc tctcgccgac tcgggttggg
     1201 cagccgccgc gccgagcagg tggtggccca tgcgggcgaa ccgttcgctg ccggcggagt
     1261 ggagcaggta gagcagggcg aagtggatcg cccacaaccg agcccgacgc cacgtgtcgt
     1321 cgtcgctgtc aacggccgag cggaagcggg cgcgggcatc gccgtcgaac agcatccacg
     1381 cgacggcgag gtcgaccgcc gggtctcccg acgtcaggtc gcccaggtcg agcacggcca
     1441 cgatccgccc gtcacgcacg atcaggttgg cgctgtgcag atcgccgtgc agccacacgg
     1501 gcggaccgtc ccattcgggg gtcgcggcca ggtggtcgac caacgccgca accactgcac
     1561 ggtgagcgcc gtcgagcccg agccgggcaa ggttggcatg caccctgggc accagctcgc
     1621 cgaccggctg gccccgaaac ggattctccg gcgcatcggc cggtgctggc tggtgcaggg
     1681 cacggacgaa cgcgcccagg cgatcggctt cacccaccgg gtcggccaac gcggtgtccg
     1741 cggcgacctc accgtcgaac cacggacaga tgctccagtg ccacgggtag tcagcggcgg
     1801 gttgcccgaa ccggaccggc gcggcgatcg gaatcggcaa ccgagtggcc aacgtcggga
     1861 gccagcgctg ctcgttgagg accagatcgg caccggcctg gcgtcgcggc agcctggcca
     1921 ccaggtcgtc cccgacacgg acgatcacgt tgtcccaccc gttggcgacg atggtcaacc
     1981 gctgcccggc caaatcggga tgttggtcgg ccaacagtcg ccgcaccagt tccaccgtgg
     2041 gcgccgcatg ttggaaaccc cgcgcccgcg cccggttcaa cgaaaagacg gtttcgccac
     2101 ccaacgaggt ctgcacctgg
//
LOCUS       DAORNF010000620         2112 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_39085_length_2112_cov_1.649976, whole genome shotgun sequence.
ACCESSION   DAORNF010000620 DAORNF010000000
VERSION     DAORNF010000620.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39085_length_2112_cov_1.649976"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            119..>360
                     /locus_tag="PLV68_18915"
     CDS             119..>360
                     /locus_tag="PLV68_18915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03375.1"
                     /translation="MKLFATRHRATPRRNKQRLVRKRSKLRLLDLVDETLAGIFARPL
                     RALLTTLGTVLGLASLVATLGISRTSGSQIVDRFNEL"
     assembly_gap    361..376
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            388..>946
                     /locus_tag="PLV68_18920"
     CDS             388..>946
                     /locus_tag="PLV68_18920"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRE03376.1"
                     /translation="MRVRSSGNSDQQQQQSAAGTSLPWNVESRLDQMNGVVASGAVAP
                     VSNPGGVRTVPVIDPTSVATQTVPVIATSSGYLAAVRGHIAAGRMYDAGHIARRDRVV
                     VIGADLADSLGITSVANQPGLYIGEELFVVIGIVDYSARDSGITGSILVPATVAEARF
                     GVERPERVVIETELGAAGVIAAQAAM"
     assembly_gap    947..962
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <963..>1280
                     /locus_tag="PLV68_18925"
     CDS             <963..>1280
                     /locus_tag="PLV68_18925"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HRE03377.1"
                     /translation="PAEPTRQRDAVASDVNVLFILLGLISLVVGAIGIANVTLVTVME
                     RTGEIGLRRALGARRRHIAGQILCESAAMGLVGGIVGASAGIVVVVAVSAQRQWTPLL
                     DPW"
     assembly_gap    1281..1319
                     /estimated_length=39
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1434..>1688)
                     /locus_tag="PLV68_18930"
     CDS             complement(1434..>1688)
                     /locus_tag="PLV68_18930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03378.1"
                     /translation="MPPQASGLNQAQILAVVCHERYDLGGISPTDPDFADEFELWCSP
                     ESEIYLGLQDGSLTFDNLHEQVEGVLPVLTTARPGTPADA"
     assembly_gap    1689..1698
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1699..>2112)
                     /locus_tag="PLV68_18935"
     CDS             complement(<1699..>2112)
                     /locus_tag="PLV68_18935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03379.1"
                     /translation="AKAAKAAPTGPPPPKPDIPVVAAAKQRKKIPFWAMATIGLLPVW
                     GFMYVQGVKPAEKHVSGPLAEGTTIYSASCSTCHGATGGGGVGRPFTEGHAATTFPHI
                     EDQLNLVYVGTQGYIDANLASYGDPNVGHLTYNGAL"
BASE COUNT          278 a          649 c          778 g          326 t
ORIGIN      
        1 tgttcgacga gctgctcgcc gacgggctga cgatcgcgct gatcacccac gacaacgacg
       61 tggccgtgcg ggccaagcgc cgggtgcgca tcgtcgacgg cacgttggag gagatcgggt
      121 gaagctgttt gccacgcgtc atcgcgccac accgcggcgc aacaagcagc ggctggtccg
      181 caagcgcagc aagctgcgtc tgctcgatct cgtcgacgag acgttggcgg gcatcttcgc
      241 ccgcccgctg cgggctctgc tgaccaccct gggcacggtc ctggggttgg cgtcgctggt
      301 cgccacgctg ggcatctcgc gcacctcggg cagccagatc gtcgaccgct tcaacgagct
      361 nnnnnnnnnn nnnnnnccca ggtcaccgtg agggtgcgca gctcgggcaa ctccgatcag
      421 cagcaacagc agtcggccgc gggtacgagc ctgccctgga acgtggagag ccggctcgac
      481 cagatgaacg gcgtggtcgc gtcgggcgcg gtcgcccccg tgtccaaccc gggtggggtg
      541 cgcaccgtgc cggtgatcga cccgacctct gtggccaccc agacggtgcc cgtgatcgcc
      601 acgtcgtccg gctacctcgc ggcggtgcgc ggacacatcg ccgctgggcg gatgtacgac
      661 gccggacaca tcgcccgccg agatcgggtc gtggtgatcg gcgccgacct ggccgactcg
      721 ctcggcatca cctcggtggc caatcagccc ggcctgtaca tcggcgagga gctgttcgtc
      781 gtgatcggca tcgtcgacta ctcggcgcgc gacagcggca tcaccgggtc gatcctggtg
      841 ccggccacgg tggccgaggc ccgcttcggc gtcgagcgcc ccgagcgggt cgtgatcgag
      901 accgagctcg gcgcggccgg ggtgatcgcg gcgcaggcag cgatggnnnn nnnnnnnnnn
      961 nncacctgcc gagccgaccc gccagcgcga cgcggtcgcc agtgacgtca acgtgctgtt
     1021 catcttgctc ggcctgatct cgctggtggt gggggcgatc ggcatcgcca acgtcacgtt
     1081 ggtgacggtg atggagcgca cgggcgagat cggtctgcgc cgcgcgctgg gggcgcggcg
     1141 cagacacatc gccggacaga tcctgtgcga gtcggcagcg atgggtctgg tcgggggcat
     1201 cgtcggcgcc agcgcgggca tcgtggtggt cgtggccgtc tcggcccagc gtcagtggac
     1261 gccgctgctc gatccgtggc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt
     1321 accccagcat ccgcgcggcg aggatggaac cggtagaagc cctccgcgcc gggctgtagc
     1381 tcgtcggtcg cggctgaccg tgaagtcgtt ggtcgccgcg gcctccgcct cggtcaggcg
     1441 tcggcggggg tgccggggcg ggcggtggtg aggacgggga ggacgccttc gacctgttcg
     1501 tggaggttgt cgaaggtgag cgagccgtcc tgcaggccga ggtagatctc ggattcgggt
     1561 gagcaccaca gctcgaattc gtcggcgaaa tccgggtcgg tgggggagat gccaccgagg
     1621 tcgtagcgct cgtggcagac cacggccagg atctgggcct ggttgaggcc agacgcctgc
     1681 ggcggcatnn nnnnnnnnag cgcaccgttg taggtgaggt ggccgacgtt ggggtcgccg
     1741 taggacgcca ggttggcgtc gatgtagccc tgggtgccga cgtagacgag gttgagctgg
     1801 tcctcgatgt gggggaaggt ggtcgcggcg tggccctcgg tgaagggccg cccgacaccg
     1861 ccgccgcccg tggcgccgtg acaggtgctg cacgacgccg agtagatggt ggtgccttcg
     1921 gccaaggggc cgctgacgtg cttttcggcc ggcttgacgc cttgcacgta catgaagccc
     1981 cacacgggga gcagcccgat ggtggccatg gcccagaacg ggatcttctt gcgctgcttg
     2041 gcggcggcca cgacggggat gtcgggcttg gggggtggcg gcccggtagg cgcggccttg
     2101 gcggccttgg cc
//
LOCUS       DAORNF010000621         2111 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_39101_length_2111_cov_1.948444, whole genome shotgun sequence.
ACCESSION   DAORNF010000621 DAORNF010000000
VERSION     DAORNF010000621.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39101_length_2111_cov_1.948444"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..523
                     /locus_tag="PLV68_18940"
     CDS             <1..523
                     /locus_tag="PLV68_18940"
                     /inference="COORDINATES: protein motif:HMM:NF037895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03380.1"
                     /translation="LPTLESLAAASEHDVLKLNMDLVGLDGATWNEQSVRERTERLIN
                     VVVEIWPVPAGHKSDFGSQPDRPSRKIEVADLIGAALLESGATLFVRRKKHSHRTATV
                     LPDGRIDVDGVAYATPSGAATSITGKSENGWWFFLVDAASKKSLSDLWHEYVDQTAVD
                     VDEADMPEEEDDE"
     gene            520..>787
                     /locus_tag="PLV68_18945"
     CDS             520..>787
                     /locus_tag="PLV68_18945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03381.1"
                     /translation="MTSDLPTVWSRIRQHEGEGFQLIRGDVFTYEMFDNYLRPVGRVR
                     HLSRTNFGKALARVPLDGYQSVKDLQGPSYVYAILMDPRVRRGDW"
     assembly_gap    788..799
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <800..1150
                     /locus_tag="PLV68_18950"
     CDS             <800..1150
                     /locus_tag="PLV68_18950"
                     /inference="COORDINATES: protein motif:HMM:NF022671.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3024 domain-containing protein"
                     /protein_id="HRE03382.1"
                     /translation="DVALPETDLARIRRWVDARNARLPERARDQIRNEIDVDDRAVTI
                     LECRPPWRADFGPEWTRFPIARLRYTKARREWQIYWRDRSLKFHAYDLVPPSANVETL
                     LAEIDADPTCIFWG"
     gene            1319..>1720
                     /locus_tag="PLV68_18955"
     CDS             1319..>1720
                     /locus_tag="PLV68_18955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRE03383.1"
                     /translation="MNTSTLERTVLPPVESLDALVAMLSRPGAEPTTRLSGPNGEHLV
                     LPPEVFEVLRDVVEAMASGQAVTIAPVHQRLTTQEAADLLGVSRPTLVKLLETGEIPF
                     EQPGRHRRVRLADVLAYRERASGERRAALDRM"
     assembly_gap    1721..1904
                     /estimated_length=184
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          354 a          617 c          601 g          343 t
ORIGIN      
        1 cctgcccacc ctcgaatccc tcgccgccgc ctcggagcac gacgtgctca agctgaacat
       61 ggatctcgtt gggcttgacg gcgcgacgtg gaacgagcag tccgtgcgtg agcgaacgga
      121 gaggctgatc aacgtagttg tggagatctg gccggttccg gccggtcaca agtccgactt
      181 cggctcacag cccgatcgcc caagccgaaa gatcgaagtc gccgacttga tcggcgcggc
      241 gcttctcgag agcggggcca cgctcttcgt gcgtcgcaag aagcacagtc accgcaccgc
      301 gacggtgcta cctgacggac gaatcgacgt cgacggcgtt gcctacgcca cgccgtcagg
      361 tgcagcgacg tcgatcaccg ggaagagcga gaacggatgg tggttcttcc ttgtcgacgc
      421 cgcaagcaag aagtcactca gcgatctctg gcacgagtac gtcgatcaga ccgcagtcga
      481 tgttgacgag gccgacatgc ctgaggagga ggacgacgaa tgacatctga tctgccaacg
      541 gtgtggagca gaatccgcca acacgaaggc gagggcttcc agctcatccg cggggatgtc
      601 ttcacctacg agatgttcga caactacctg cgccccgtgg gtcgtgtgcg tcacctgtcg
      661 aggacgaatt ttgggaaggc cctcgcccgt gtcccgctgg acggctatca gtccgtaaag
      721 gaccttcaag gtccctcgta cgtctacgcc atcctcatgg acccgcgggt tcggcgcggc
      781 gactggtnnn nnnnnnnnng acgtggcact tcccgagact gacctcgccc gcatccgacg
      841 gtgggtcgat gcgcgcaacg cccgactgcc ggagcgcgct cgtgaccaga tccgcaacga
      901 gatcgatgtc gatgaccgtg ccgtgacgat cctcgagtgt cgacctccgt ggcgagccga
      961 cttcggtccc gagtggacac ggttcccgat tgctcggctg cgctatacga aggcgcgccg
     1021 agagtggcag atctactggc gcgatcggag cctcaagttc cacgcctacg accttgtccc
     1081 gccgagtgcg aacgtcgaga cgctgctcgc cgagatcgat gctgacccca cgtgcatctt
     1141 ctggggctga ggcgaacgat tctcaagtct gcaaccgttg ctcccgaggc ggctaagcaa
     1201 cgcttgttga gtaaccgttc cttagcctcg accggagaac tgctcctcgg tgtcgtgcgc
     1261 aggcacttgc gaatttttcg atttgcgcta cgatgacggc ctgaggagga gcacccacat
     1321 gaacacgtcc acgctcgaac gcacggtttt accgccagtc gaatcgcttg acgcgctcgt
     1381 cgcaatgctc agccggcctg gtgccgagcc caccacgagg ctgtcaggac cgaacggtga
     1441 gcatctcgtg ctgccgccgg aggtcttcga agtcctgcgg gacgtcgttg aggcgatggc
     1501 aagtggccaa gcggtgacta ttgcgcccgt acatcagcgg ctgacgacgc aagaggccgc
     1561 cgacctgctc ggcgtcagcc gacccacatt ggtgaagctc ctcgaaacag gggagatccc
     1621 cttcgaacag ccgggacgcc atcgcagggt gcgccttgcc gacgtgctgg cgtatcgcga
     1681 acgcgcctcg ggtgaacgcc gtgctgcact cgatcgcatg nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncaccgc aacctcgtcg
     1921 acgacggcct caacgccgct tccgtcgacc ggctcatcgc cgagatggcc gctgcgttcc
     1981 cggacgcgga agtggctggc tatcggtcgt tgatcgacgg attgagctgc gatcccaagg
     2041 atcgccacgt gctcgccgcc gccgtgaggg ccgatgcggc ggcgattgtg accttcaacg
     2101 tcgccgactt c
//
LOCUS       DAORNF010000622         2110 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_39132_length_2110_cov_1.850608, whole genome shotgun sequence.
ACCESSION   DAORNF010000622 DAORNF010000000
VERSION     DAORNF010000622.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39132_length_2110_cov_1.850608"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>288
                     /locus_tag="PLV68_18960"
     CDS             <1..>288
                     /locus_tag="PLV68_18960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRE03384.1"
                     /translation="QLTVAENLRVAHPRRAGVGGRALPATHAPISRVARSRAVRGAVE
                     DVCAVFPALGAIRGRKAGLLSGGEQQMLAIARALVTRPKVLLIDELSLGLAP"
     assembly_gap    289..326
                     /estimated_length=38
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <327..510
                     /locus_tag="PLV68_18965"
     CDS             <327..510
                     /locus_tag="PLV68_18965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRE03385.1"
                     /translation="ALARDGLGVLLVEQQINLALRSVDRGYVLRDGVIVVDAPAAELR
                     QAGDVLAAAYLGNAVG"
     gene            695..>2110
                     /locus_tag="PLV68_18970"
     CDS             695..>2110
                     /locus_tag="PLV68_18970"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRE03386.1"
                     /translation="MLVPSRIHVGDSVGVWSALLLVLLLSALLGLGLYAAVYRPMRTA
                     SLLARVVASVGVMIVLQAAVVIQFGPESRLPRSILPNETIKLFGSAFPRDRFILAFVT
                     AAVAVALWAVYRFTRFGLATRAASETERGAQLCGISPTSLALTNVIVVSVMAAAFGIV
                     ASPITGLDPTKFTLFIVPGLAAALVGRLRLISITAIAGLGIGMFQSELLFVSTQRWFP
                     AWARSGVVDSVPFVLIAVALIASGHRLPVRGVLNEDSALTALRRGERPTVSLIWLAVA
                     TVALVVLRDQYRQALTVSIIGVVLMLSFVVLTGYLGQISLAQAAVAGVSGFAVAKLST
                     EHGIGFPLAPLLAALIATVAGCIIGISGLRVRGVQLAVVTLAGAVAIERLVFNNSELT
                     GGVTGATVARPRLFGVNLSPLSATQRPSLAFGLLCVAVTALVIAAVLVIRGGAIGRRL
                     IAARSNERAASSLGIDLVRVKM"
BASE COUNT          240 a          706 c          720 g          406 t
ORIGIN      
        1 ccagttgacg gtggcggaga acctgcgcgt cgctcacccc cggcgcgccg gcgtgggtgg
       61 ccgcgccctc ccggccaccc acgcccccat ctccagagtc gctcgctcca gagccgttcg
      121 tggcgcagtc gaggacgtgt gcgcggtgtt tcccgcgctc ggcgcgatca gaggtcgcaa
      181 ggccgggctg ttgtcgggag gcgagcagca gatgctggcg attgcccgtg ccctggtgac
      241 ccgaccgaag gtactgctga tcgacgagct cagcctcggc ctggccccnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnncgca ctcgcccgcg acggcctcgg cgtgctgctg
      361 gtcgaacagc agatcaacct ggccctgcgc tccgtcgatc gcggctacgt gctccgcgac
      421 ggggtgatcg tggtcgatgc tcccgcagcc gagctgcgcc aagcaggcga cgtgctggcc
      481 gccgcgtacc tggggaatgc ggtgggctga tgtcgcagta catgttgttc ctggtgctcg
      541 gcctggggtc gggcgccgtc tacgccgggc tggggatggg cctggtggtg cagtacaagg
      601 cgctgggcat catcaacttc gcctacgggg cgatggcgat gatcgccgcg ttccagtacc
      661 acgagttgcg caccaagggc gatctcgtca tgccgtgctg gtaccgagcc gcattcacgt
      721 cggcgattcg gtgggcgtgt ggagcgccct gctgctggtg ttgctgctgt ccgcactgct
      781 cggcctgggg ctctacgcgg ccgtgtaccg accgatgcgc accgcatcgt tgctggcgcg
      841 cgtcgtcgct tcggtgggtg tgatgatcgt gctgcaggcg gccgtggtga tccagttcgg
      901 acccgagtcg cgcctgcctc gatcgatcct tccgaacgaa acgatcaagc tgttcgggtc
      961 ggcgtttccc cgcgaccggt tcatcctcgc cttcgtgacc gcggcggtag cggtcgcgct
     1021 gtgggccgtc taccgcttca cgcgcttcgg tctggccact cgcgccgcgt ccgagaccga
     1081 gcgcggcgcg cagctgtgcg gcatctcacc cacctcgctg gcgttgacca acgtgatcgt
     1141 cgtgtcggtg atggcggccg cgttcggcat cgtcgcctca ccgatcaccg gcctcgaccc
     1201 gaccaagttc acgctgttca tcgtgcccgg ccttgcggcc gcgctggtcg gccgactgcg
     1261 cctcatctcg atcaccgcca tcgcaggttt gggcatcggc atgttccaat ccgagttgct
     1321 gttcgtgagc acgcagcggt ggttcccggc ctgggctcgg tcgggtgtgg tcgactcggt
     1381 gccgttcgtg ctgatcgccg ttgcgttgat cgcgtccggg catcgtctgc ccgtgcgcgg
     1441 cgtgctcaac gaggacagcg ctctcaccgc gctgcgccgc ggcgagcggc caactgtgtc
     1501 gttgatctgg ctcgcggtcg ccacggtggc cttggtggtt ctgcgcgatc agtaccgcca
     1561 ggcgctgacc gtcagcatca tcggggtcgt cctcatgctc tccttcgtcg tgctgaccgg
     1621 ctacctggga cagatctcgc tggcgcaggc cgcggtcgcg ggcgtgtccg gcttcgccgt
     1681 agccaagctg tccaccgagc acggcatcgg ctttccgctc gctccgttgt tggccgcact
     1741 gatcgccacg gtggccggat gcatcatcgg catctccggt ctccgtgtcc gcggcgtgca
     1801 actggccgtg gtcaccctgg ccggggcggt ggccatcgag cggctggtgt tcaacaacag
     1861 cgagctcacc ggaggtgtca cgggcgcgac ggtggctcgg ccacgcctat tcggcgtcaa
     1921 cctgtcgccg ctgtcggcta cccaacgccc aagcctggcc ttcggcctgt tgtgcgtggc
     1981 ggtcacggcg ctggtgatcg cggcggtgct cgtcatccgt ggcggcgcga tcggccggcg
     2041 gttgatcgcg gctcgctcca acgagcgcgc cgcgtcgtcg ctgggcatcg atctggtccg
     2101 cgtcaagatg
//
LOCUS       DAORNF010000623         2110 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_39135_length_2110_cov_1.696350, whole genome shotgun sequence.
ACCESSION   DAORNF010000623 DAORNF010000000
VERSION     DAORNF010000623.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39135_length_2110_cov_1.696350"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..410)
                     /locus_tag="PLV68_18975"
     CDS             complement(3..410)
                     /locus_tag="PLV68_18975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03387.1"
                     /translation="MFAAGMAFAIGWYILVGFARARPGELLHVDDFNRSEADPQSGMI
                     RCGFLVDWTRIKDPVGRVEVDRYVFRFFGRRTPAVDVPWSRSTAEVRFRTLPLGMAVF
                     NIEGLDGKAATMWCSKRSARRVMALAGLKQADH"
     gene            complement(484..1029)
                     /locus_tag="PLV68_18980"
     CDS             complement(484..1029)
                     /locus_tag="PLV68_18980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03388.1"
                     /translation="MLYFGTVIQSEPQVVPPTTSLKAVSPIVLAAACRNYSDWAGDRV
                     VTSNITIVDSPRLLRSAELLSTSFLGDQYALQAENLLMEGLDTEAIVARLEEDGASND
                     QIARAKLYLDERELPPSESASQRAIIYAVAHTAKSVLVVRFVPADVSHKIDKPLSRVG
                     PIRIQLPDGSVTEIEESTCWR"
     gene            complement(1760..1954)
                     /locus_tag="PLV68_18985"
     CDS             complement(1760..1954)
                     /locus_tag="PLV68_18985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03389.1"
                     /translation="MNERAEQIADELDRLADELAEMAIADLTNTVRTGGQRSSPEERR
                     LTQARRALAKAATLLRTPDE"
BASE COUNT          425 a          656 c          634 g          395 t
ORIGIN      
        1 catcagtgat cagcctgctt cagtcccgcc agtgccatca ctcgccgtgc cgaccgcttg
       61 gagcaccaca tcgtcgccgc cttcccgtcc agtccttcga tgttgaacac cgccataccg
      121 agcggcagcg tccgaaaccg cacttcggcc gtgctgcgcg accacggtac gtccactgcc
      181 ggggtccgac gcccgaagaa tcgaaataca tatcggtcga cttcgacccg tccgaccgga
      241 tccttgatgc gtgtccagtc caccaagaac ccgcagcgaa tcatgcccga ctgaggatcg
      301 gcttcggatc ggttgaaatc atcgacgtgc agtagctcgc ctggtcgcgc ccgggcgaag
      361 cccacaagga tgtaccaacc aatcgcgaac gccatgcccg cggcgaacac gacgtggagc
      421 ggagagagtg ccatcacgtg cggttcctac tccagcagca tctcggcgac aagggcggac
      481 gagttacctc cagcatgtgg attcttcgat ctcggtgaca gagccatcgg gtagctgaat
      541 gcggattggc ccgacacggc tcagcggctt gtcaatcttg tgcgacacat cggccggcac
      601 gaacctgacg accagcaccg acttcgctgt gtgggcaacc gcgtagatga tcgctcgctg
      661 agatgccgac tccgacggcg gcaactcccg ctcgtcgagg tagagcttcg cgcgtgcaat
      721 ttggtcgttc gatgccccgt cctcttccag gcgcgcgacg attgcttctg tgtcgaggcc
      781 ttccatgagc aagttctcgg cttggagagc gtactgatcg ccgagaaatg acgtgctcaa
      841 cagttctgcc gaccggagca gcctgggact gtccacaatc gtgatattgc tcgtcaccac
      901 tcgatcgccc gcccagtcgc tgtagttgcg acaggcagca gccaggacaa tcggtgagac
      961 agccttgaga ctcgtcgtag gcggcacgac ttggggctcg ctctgaatga ccgtcccgaa
     1021 gtagagcaca tcccggccac ttttctcgac gggcgcggct gatggctccc atcgacttag
     1081 cagtattacc gcgccaacag cgaacgctac tacgacgacg ccactccaaa agtgaggatg
     1141 gcgccacggg cgagtaaacc acatcatgta acaccatcca atgcgccgca acatggaata
     1201 atctcgttga gcagcccagg agctatttcg gggcctctat ctatgagggc aattccatac
     1261 gtccatcctg ttgagaggga tacactaaat ccaggttgag cagcgacact aaggcttgca
     1321 ccaacagtcc agtatccgat gtagctccaa tcattgtcga aacttccgca caccgtaagc
     1381 gccccggccg cgagaatcgc accactcgcc gttccgcaca tagtattgcc tatgagattc
     1441 tgaagttcta atgcgtttgt aacgaagcta gtgactgctg cggaggcccc cggtgttccg
     1501 attccgtcag cgacaaaggt cataagccca gtgccagatg gcaaagacgc aacacaaggg
     1561 gatcgtcctc ctgttgctcg attggaacgg gctccaccgg cacgggctcg gcggctgcgc
     1621 ccgacaggcc tatttcgccg acgggagcgg acatcaacac caaaacagca agacgtttca
     1681 cagcaacccc caaagagctt ctgttgagct aaacgcgcca gatgagcagc agtcaacttg
     1741 gagggttgct caacgacctc tactcatctg gggtgcggag gagggtggcg gctttggcta
     1801 gtgctcggcg ggcttgggtg aggcggcgtt cttcggggct ggagcgttgg ccaccggtgc
     1861 ggacggtgtt ggtcaggtcg gcgatggcca tctcggcgag ttcgtcggcc aggcggtcga
     1921 gctcgtcggc gatctgttcg gcacgctcgt tcacgacccg cgccccgccg ctgtggtcgt
     1981 gctgtcgaag tcggcaaggt cagcgatgag gtcgaccggg tgggcgaccg ccaggccggt
     2041 cgcggcgagg tgcatcgcgc agccggggtt ggcgctggcg acgacggcac cgggcgcgat
     2101 cacgtgggcg
//
LOCUS       DAORNF010000624         2110 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_39137_length_2110_cov_1.652068, whole genome shotgun sequence.
ACCESSION   DAORNF010000624 DAORNF010000000
VERSION     DAORNF010000624.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39137_length_2110_cov_1.652068"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    311..401
                     /estimated_length=91
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(741..1247)
                     /locus_tag="PLV68_18990"
     CDS             complement(741..1247)
                     /locus_tag="PLV68_18990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03390.1"
                     /translation="MSSGGWFQYGPGPVRLRRSSAFSIVRRVRGGPFRMIELNILGWS
                     ALLAPSALIGGLVALALGRPVAVGALGVAGAALAVAVAWDRWRWWNSVVSVSSDLDLD
                     ELRGVVDCLRVGGVDVTVDAADSGLVVAPGSDRNEDVDPWRLTTHQRWVNRVLAALID
                     ASAARPPY"
BASE COUNT          366 a          725 c          650 g          278 t
ORIGIN      
        1 atgcgccagt tggtcgagct cgacccgtac gacgagtcgg cccatctcgg gctggtctcg
       61 gcacttcgtt cgatgggcgc ccacggccag gcccaggcag cctttcgctg ctatgtcgag
      121 cgcatggccc aactcggcct cgacgtcgaa tcagcagcag cagcagcgca ataggcaccc
      181 acctctgcct cgactcgccg ccagacgaac cgccccgcac cgccttcgcg gtcgacgacg
      241 cgcttgacgt cgtacgagag cacctcgggt gcgcagtgga actcgtggat ggcgacgagc
      301 ccaggcgatg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncgagcgcag cagcgtcgat
      421 ttgccgaacc ctcgcggccc ctcgagcagg atcgctggcc cctcgtcgac cagttcgtcg
      481 acgaacggtt ccgcccaccg ttgcacgtgc tcgatcgtgg ggatcacacc ggcgaccctg
      541 gtcggagttg aagaatcgtc cagccgtgtc ctgccagacg ctatcgggcc cgccaaagcg
      601 ggcggacaac gacctggtgc caccagcggg taggaggccc acgaccatcc atcgggttgc
      661 cgccgagagc acaccggcgt ccctgagttg ctcccggtgg aactcccgga cttgctcccg
      721 attgcctgcc aacgtgctcg tcagtacgga ggacgagccg ccgatgcgtc gatcagggcg
      781 gcgagcacgc ggttcaccca gcgctgatgt gtagtgagcc tccacggatc gacgtcctcg
      841 ttgcggtctg agcccggcgc cacgaccagg ccggaatcgg cggcatcgac ggtgacgtcg
      901 acgcccccga cgcggaggca gtcgacgact ccccgcagtt cgtcgaggtc gagatcgctc
      961 gataccgaca ccacgctgtt ccaccagcgc caccggtccc aggccacagc caccgccaac
     1021 gccgcgccag caactcccaa tgcgccgaca gcgacggggc gaccaagagc gagcgccacc
     1081 aagccgccga tcagggccga cggagccagg agcgccgacc atccaaggat gttcagctcg
     1141 atcatccgga acgggccgcc gcggacccta cgcacgatcg agaaagcaga tgagcgccgc
     1201 agacgaaccg ggccgggccc gtattggaac caaccaccgc tgctcacgcc gcccatcgtc
     1261 ccaccccggg tcggctggcg acgcccgaga atcgtgcaca ccgtcccggt gattatctgt
     1321 gagtgctgcc cgatcttgct cccgatgaac gattggatcg gcgcgcctcg agtcggcgaa
     1381 acgtatggcg gccgatcctc tcgcgacacc aggtgtcgtg ccggcccccg acctgtcgca
     1441 gatcgcggca cagcggggtg cgtacagaga catgcgcgcc gaatcaccct ccgtccgctc
     1501 catgggacac ctgaacgaca cgtccgctga cgcgtcgtca acaggcccgg cagcatcggg
     1561 cgcgggcacg tcggcggacg cggcgcaggc ccgatggtct accgagggcc cgaggatcgt
     1621 cggactccgt tccggcacaa tgctcgatcc gaaactcgtg gccgagctcc gggcggtcgc
     1681 tggtcccacc gacatcgcac agcgggtcct gacgatcctc gagccgggcc gctgcccgcc
     1741 gacacgaaag ctgaagcgcg actgacctgc cgcgcgcccg acggcctgga gcagtccagt
     1801 ggtaccgttc gagcggagcc tctcagtgcg gcggtgaata ccggtcgaac gaactcgccg
     1861 acaacacgac cagccgcttc ctgaagtgat cacgtcgcgc tgagcagccc ccacacgtag
     1921 gactccagaa gggagtcccg agcacccgag gagcgagcgc agcacatgac ggtgaggcga
     1981 cagtcgaacg tccgcacagc ggcaggcacg ctgttcgggc gtgccgcgat ctacgcccgg
     2041 atctcgttgg acaaggtggg cgaggcggcg ggggtcgacc gacagatcga ctcgtgccgg
     2101 cacctggccg
//
LOCUS       DAORNF010000625         2108 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_39184_length_2108_cov_1.356064, whole genome shotgun sequence.
ACCESSION   DAORNF010000625 DAORNF010000000
VERSION     DAORNF010000625.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39184_length_2108_cov_1.356064"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    259..299
                     /estimated_length=41
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<300..1182)
                     /locus_tag="PLV68_18995"
     CDS             complement(<300..1182)
                     /locus_tag="PLV68_18995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03391.1"
                     /translation="MVVILAVRKDVMMRFAMLMLLALAGAAADVRGQTDSPQVDQAEV
                     LRLGDTVQHVGDRGATAEPADAFVAAMSPPESDADKWFISVLEMRGCAACAQLERDWA
                     TDEWLLALAVPDDPAKSWAHFRKYDKDDASQQFRWEGIKVSAYPTILVQPPRSGRYGD
                     AATVVYQGVYQGEPQRLARGISTAIRRYVARLEATRSAGDLGPIGQSAADPPWQPAPR
                     VTPGGPKPDGPFPLLDPNIPPLPEPVAPPAAAFPWTAVLTAAFAGFTVPTAVALAAWV
                     LVLVRERRRAARQPLLLD"
     assembly_gap    1222..1231
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1232..1424)
                     /locus_tag="PLV68_19000"
     CDS             complement(<1232..1424)
                     /locus_tag="PLV68_19000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03392.1"
                     /translation="MRWAAETGRGAAIGAGTAHFQTLMGFEPTSGTWYVCNNNSPQRI
                     DVYDDAAFRRLHLASGQWVV"
     assembly_gap    1523..1610
                     /estimated_length=88
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1818..>2108)
                     /locus_tag="PLV68_19005"
     CDS             complement(1818..>2108)
                     /locus_tag="PLV68_19005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03393.1"
                     /translation="SGEKPMKRLLFLLACGLFVVTPFPTASAACDHAASCRPSVRIAP
                     RRVVRIPIRLIRSLRRRDCRCETCGHFDPLPRARMPYGGSDSDCAAGVCLPR"
BASE COUNT          324 a          678 c          675 g          292 t
ORIGIN      
        1 ccatcgagta ccagctcggt ggcctgaatc aggtaatccc ggtcggtccg ttcgatccgc
       61 acgccgccgt aaccgtcatc gagttggagc aaggtccgtc cgaccacggc ggacacggcg
      121 acctcgctcg catcgcgccg gtagaggact tcgcgtgagg cgtgggcctt cagctgtcgg
      181 gctaaccagt tctgtccgag ctgcaggcga tcgaccatcg catcacgtgc cgctcgacga
      241 ggtcggccgc tgctgaccnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng
      301 atcgagcagc agcggttggc gggcggcgcg gcgccgctcg cggacgagca ccaacaccca
      361 cgccgcgagc gccacggcgg tcggtaccgt gaacccggcg aacgccgcgg tcagcaccgc
      421 cgtccacgga aacgcggctg cgggaggagc gaccggttcc gggagcggcg ggatgttcgg
      481 gtcgagtagc gggaacggtc cgtccggctt cggtccgccg ggcgtcaccc gcggcgccgg
      541 ctgccacggc ggatcggcgg cggactggcc gatagggccg aggtcgcccg cggaacgcgt
      601 cgcttcgagc cgagcgacgt accggcgaat cgccgtgctg atgccgcggg ccaatcgctg
      661 cggctcgccc tgatagaccc cctgatacac gaccgtcgcg gcatcaccgt agcgtccgct
      721 tcgcggcggc tggacgagga tcgtggggta ggcggagacc ttgatccctt cccagcgaaa
      781 ttgctgactc gcatcgtcct tgtcgtactt gcggaagtgc gcccaggact tcgcgggatc
      841 gtcgggtacg gcgagcgcca gcagccattc gtcggtcgcc cagtcgcgtt cgagctgggc
      901 acaggcggca cagccccgca tctcgagcac gctgatgaac cacttgtccg catcgctctc
      961 gggcgggctc atcgccgcca cgaacgcatc ggccggttcc gcggtggcgc cacggtcacc
     1021 gacgtgctga accgtatcac ccagtcgcag cacctcggcc tgatcgacct gcggggagtc
     1081 ggtctggccc cgaacatcgg ccgccgcgcc cgcgagggcg agcagcatca gcatcgcaaa
     1141 ccgcatcatc acgtcctttc gtactgcgag gatcaccacc accggtagta ccgcggccgc
     1201 tccggatgcg gcgggtaatc gnnnnnnnnn ntcacgaccc attggccgct cgccagatgc
     1261 agtcgacgga acgccgcgtc gtcgtagacg tcgatccgct gcggcgagtt gttgttgcag
     1321 acgtaccagg tgcccgacgt cggctcgaag cccatcagcg tctggaagtg ggcggtcccg
     1381 gcaccgatcg ccgcaccacg tccggtctcg gctgcccacc gcatccagtc ccaggtttcg
     1441 gtcccggtga tgttgaaggc gcgaatgccg cgccggcggc agtactccga gacgcgcgaa
     1501 ggacccgagc cgccccgttc gcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gcacgagccg
     1621 tccggattgc ggaaccaggt gcggatctcg cgcgggaggt cgacggccag tcgatcgtcg
     1681 gggcattcga ttgtctgtgc gatcagcgcg tccgagccgc cgagcaacag aatcgcggcg
     1741 agggggatca agaacttcac gcgggcttct ccctgtcgag gaaccgagct cgtcggagcg
     1801 ggatcgggac gccgaactca ccgcggcaga cagactcccg ccgcacaatc cgagtcgctg
     1861 ccgccgtacg gcatccgggc ccggggaagc ggatcgaagt ggccgcacgt ctcgcagcgg
     1921 caatcacggc ggcgcaacga acggatcagg cgaatcggaa tccgcacgac tcgtcgtggc
     1981 gcgatccgca cgctcggtcg gcacgacgcc gcgtggtcgc aggccgccga tgcggtgggg
     2041 aacggcgtga cgacgaacag gccgcacgcc agcaggaaca acagtcgttt catgggtttc
     2101 tctcctga
//
LOCUS       DAORNF010000626         2103 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_39295_length_2103_cov_1.792480, whole genome shotgun sequence.
ACCESSION   DAORNF010000626 DAORNF010000000
VERSION     DAORNF010000626.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39295_length_2103_cov_1.792480"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1547
                     /locus_tag="PLV68_19010"
     CDS             <1..1547
                     /locus_tag="PLV68_19010"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HRE03394.1"
                     /translation="AELDLGPSAKRGAWTLPPANSLVRSGVQTVNRPEAEARGRTLVE
                     SLNSHGVETKLVGMTVGPPVTRYELELGAGVKVARVTSLNRDIAYAMAATDVRILAPI
                     PGRSAIGIEVPNHTRQLVSLGDVMASAEAKAATQPLDVAIGKDIAGRSVFLNLATTPH
                     LLIAGATGAGKSSGINCIITSLLMRNSPDAVKLILIDPKQVEMGQYNRIPHLLTQPVT
                     NPKKAANALQWACKEMDRRYDVLFEVGFRDIGGYNAAFDRGDLQPEPGQEQTLQRMEY
                     IVVVVDELNDLMMVAQRDVEESITRIAQKARAVGIHLIIATQRPSVNVITGVIKANVP
                     ARMAFAVSSLTDSRVVLDQPGAEKLVGKGDMLLLPGNSNVAQRIQGCWVSEDEVRKVV
                     AHWRRQAPEVTYVSGVEGDEDGGGGGLFGLTGGSSGGSSSGGGDDEDDDLLRQAMELV
                     VRSQLGSTSMLQRKLKVGFARAGRIMDLLETAGVVGPSEGSKARAVLMTVEEFELLQD
                     AQRNRT"
     gene            1678..>2103
                     /locus_tag="PLV68_19015"
     CDS             1678..>2103
                     /locus_tag="PLV68_19015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HRE03395.1"
                     /translation="MPELELVEVTSGLRFPEGPIAMADGSVLVVEMFGPRITRVAPDG
                     AKTTVAEIAGGPNGMALGPDGAIYLCNNGGTFSPLEIGDWLVPGPFDAGRYIGGRIQR
                     VDIATGVVTDLYTECDGRPLRGPNDIVFDADGGMWFTDHG"
BASE COUNT          361 a          709 c          713 g          320 t
ORIGIN      
        1 aagccgaact cgacctcggc cccagcgcca agcgcggggc gtggacgttg cctcctgcca
       61 actcgctcgt ccgctcgggg gtgcagaccg tcaaccgacc agaggccgaa gcgcgcggcc
      121 gcacgctggt cgagtcgctc aactcgcacg gggtcgagac caagctggtc ggcatgaccg
      181 tcggcccgcc ggtcacccgg tacgagctcg agctcggtgc tggcgtcaag gtcgcccggg
      241 tcaccagtct caatcgcgac atcgcctacg ccatggccgc gaccgacgtg cgcatcttgg
      301 ccccgatccc ggggcgctcc gcgatcggca tcgaggtgcc caaccacacc cgccaactcg
      361 tgtcgctggg cgacgtcatg gcctcggccg aagccaaggc cgccacccag ccgctcgacg
      421 tggcgatcgg caaggacatc gccgggcgat cggtgttcct caacctggcg accacacccc
      481 acctgctgat cgccggagcc accggcgcgg gtaaatccag cggtatcaac tgcatcatca
      541 cgtcgttgct catgcgcaac tctcccgatg ccgtcaagct gatcctgatc gaccccaagc
      601 aggtcgagat gggccagtac aaccgcatcc cgcacctgct cacccagccg gtcaccaacc
      661 ccaagaaggc cgccaacgcg ctgcagtggg cctgcaagga gatggaccga cgctacgacg
      721 tgttgttcga ggtcggcttc cgcgacatcg gcggctacaa cgcggcgttc gaccgaggcg
      781 atctgcagcc cgagccgggc caggagcaga cgttgcagcg gatggagtac atcgtcgtgg
      841 tcgtcgacga gctcaacgac ctgatgatgg tggcccagcg cgacgtcgag gagagcatca
      901 cccgcatcgc ccaaaaggcc cgtgcggtcg gtatccacct catcatcgcc acccagcgcc
      961 cgagcgtgaa cgtgatcacc ggtgtgatca aagccaacgt cccggcgcgc atggccttcg
     1021 ccgtcagcag tctgaccgac agccgcgtcg tgctcgatca gcccggggct gagaagctgg
     1081 tcggcaaggg cgacatgctg ctgctgccgg gcaactctaa cgtcgcccag cgcatccagg
     1141 ggtgctgggt cagcgaagac gaggtgcgca aagtggtcgc ccactggcgc cgccaggcac
     1201 ccgaggtcac ctacgtctcc ggcgtcgagg gtgacgaaga cggcggtggc ggtggcctgt
     1261 tcggcctgac cggcggttcg tcggggggca gcagcagcgg tgggggagac gacgaagacg
     1321 acgatctgct gcgccaggcg atggaactgg tggtacgcag ccaactcggg tcgacgtcga
     1381 tgctgcagcg caagctcaag gtcggcttcg ctcgcgccgg tcgaatcatg gacctgctcg
     1441 aaaccgccgg cgtcgtcggg cccagcgaag gcagcaaggc ccgcgcggtg ttgatgaccg
     1501 tcgaagagtt cgaactgctg caagacgccc aacgcaaccg cacctgaccg ttcagcccag
     1561 tcgcctggtt cgtcgggtcg cctgcttcgg gtcgcctgct tcggatcgtg cgtggccggt
     1621 ctggaacaat cgcccacgcc ggttcggtgc cggtgatcgt gcacaggggg ttcggtgatg
     1681 ccggagttgg agttggtcga ggtcacgtcg gggttgcgct tcccggaggg accgatcgcg
     1741 atggccgatg gatcggtgct ggtcgtggag atgttcggcc cacggatcac gcgggtcgcg
     1801 ccagacggcg ccaagaccac ggtggccgag atcgccggtg gccccaacgg catggccctc
     1861 ggccccgacg gggcgatcta cctgtgcaac aacggcggca cgttcagccc gttggagatc
     1921 ggtgactggc tggtgcccgg acccttcgat gcaggtcgct atatcggggg gcggatccag
     1981 cgggtcgaca tcgccaccgg ggtggtcacc gacctgtaca ccgagtgcga cgggcggccg
     2041 ctgcgcggcc ccaacgacat cgtgttcgac gccgacggcg gcatgtggtt caccgatcac
     2101 ggc
//
LOCUS       DAORNF010000627         2097 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_39440_length_2097_cov_1.276200, whole genome shotgun sequence.
ACCESSION   DAORNF010000627 DAORNF010000000
VERSION     DAORNF010000627.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39440_length_2097_cov_1.276200"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="PLV68_19020"
     CDS             complement(<1..495)
                     /locus_tag="PLV68_19020"
                     /inference="COORDINATES: protein motif:HMM:NF015649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HRE03396.1"
                     /translation="MNIRLLGTLELVDGDGNQIDIGGTQPRIILAMLVAARGKLVATD
                     ALIDTVWDEEPPHSAAGTLQTYVSRLRRALARVGGSIIHETGGYRLTVDWNTIDVHRF
                     EVLGDRARAALDGGDASAARELLIEAERLCRGPALVDVRDRARLAGYARRFDDRRLAA
                     LEDRI"
     assembly_gap    607..655
                     /estimated_length=49
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<656..>994)
                     /locus_tag="PLV68_19025"
     CDS             complement(<656..>994)
                     /locus_tag="PLV68_19025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HRE03397.1"
                     /translation="VADAAGISRRGLYLHFPSRAQLCMALVGHIDAILGLAESVRPMA
                     EATSALAMLDAWAEHVAGYHSRLVAIVRVMDRSRHDDPDVAAPWDAAMAVWHESCRSI
                     TDRLGTEGHLD"
     assembly_gap    995..1072
                     /estimated_length=78
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1350..1397
                     /estimated_length=48
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1398..>1653)
                     /locus_tag="PLV68_19030"
     CDS             complement(<1398..>1653)
                     /locus_tag="PLV68_19030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HRE03398.1"
                     /translation="HGAKLLYAYCEATVPRIQIITRKAYGGAYVVMDSKSIGSDLSYA
                     WPTAELAVMGPQGAVEIVYRRELQQAADPVGRRAELVADYT"
     assembly_gap    1654..1736
                     /estimated_length=83
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1737..>2097)
                     /locus_tag="PLV68_19035"
     CDS             complement(<1737..>2097)
                     /locus_tag="PLV68_19035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HRE03399.1"
                     /translation="DEKACLDDVRFLLSFLPANNLEEAPPVETADDPERLCPALAEIL
                     PASPNQPYDMKRVIREVVDDGEFFEYASHFAKSIVCGFARIDGKPVGVVGNQPLMLAG
                     VLDIDSSSKAARFVRTCD"
BASE COUNT          297 a          631 c          621 g          290 t
ORIGIN      
        1 gatgcggtcc tccagcgcag cgaggcgccg gtcgtcgaac cgccgcgcgt atccggcgag
       61 tcgggcccga tctcgcacat cgaccagtgc cggaccccgg cacagccgct cggcttcgat
      121 cagcaactcg cgagcggcag acgcatcgcc gccgtcgagc gcggcccgag cccggtcgcc
      181 gagcacctcg aaccggtgca cgtcgatcgt gttccagtcg acggtaaggc gatacccacc
      241 cgtctcgtga atgatcgatc cacccacccg ggccagcgcg cggcgcaggc gcgagacgta
      301 ggtctgcagc gtgcccgccg ccgaatgcgg gggctcctcg tcccacacgg tgtcgatcag
      361 cgcgtcggtg gcgacgagct tgccgcgagc ggcgacgagc atggcgagga tgatgcgcgg
      421 ctgggttccg ccgatgtcga tctggttgcc gtcgccgtcg accagttcca gcgtcccgag
      481 caggcggata ttcatccgtc ttcccgatca accgtgcaca acgtgcgccg cgccagcatc
      541 cgcagacggg ccgcgtactg ctcgacggtc cagtcgcgat cgccgacgag atcgtcgagg
      601 aactcgnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngtcga
      661 gatgcccctc ggtgccgagg cgatcggtga tcgaccggca cgactcgtgc cacacggcca
      721 tcgctgcatc ccacggcgcg gccacgtcgg gatcgtcgtg gcgactgcga tccatcaccc
      781 gcacgatcgc gaccaaccgt gagtggtatc ccgcaacgtg ttcggcccac gcatcgagca
      841 tcgccaatgc actggtcgcc tcggccatcg gtcggaccga ttcggccagc ccgaggatgg
      901 cgtcgatgtg cccgacgagc gccatgcaca actgcgcccg cgaggggaag tgcagataca
      961 gcccgcggcg gctgatgccc gctgcgtcgg ccacnnnnnn nnnnnnnnnn nnnnnnnnnn
     1021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncgcgtgtc
     1081 gcggctgcgc tgattctgcg gggtctcgat ccgatccacc gacccatcca accacaaact
     1141 acacaccatg taaagtttga gaatgaagcg ctagcgacga gccgcagtga gggagcgggc
     1201 aaagggccct ctgggcccgg agcccagtcc cgaacaccta taacggcatg ttgccgtgct
     1261 tgcggcgggg gagctcttcg cgcttggtgc gcagcatccg taggccggcg acgatcttct
     1321 ggcgggtttc cgccgggtcg atcacgtcgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnggt gtagtcggcc accagctctg cccggcgccc gacagggtcg
     1441 gcggcctgtt gcagttcgcg ccggtacacg atctcgaccg cgccctgcgg gcccatcacg
     1501 gccagttcgg cggtgggcca cgcgtacgac aggtcggagc cgatcgactt gctgtccatc
     1561 accacgtagg cgccgccata ggccttgcgg gtgatgatct gaatccgtgg caccgtggct
     1621 tcgcagtagg cgtagagcag cttggcgccg tggnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnatca
     1741 caggtccgca cgaagcgggc ggccttgctg gagctgtcga tgtcgagcac cccggccagc
     1801 atcaacggct ggttgccgac cacgccgacc ggcttgccgt cgatgcgggc gaacccacac
     1861 acgatgctct tcgcgaagtg cgaggcgtac tcgaagaact cgccgtcgtc gacgacctcg
     1921 cggatgaccc gcttcatgtc gtacggctgg ttcggcgagg cgggcaaaat ctcggccagc
     1981 gcggggcaca gccgctcggg gtcgtcggcg gtctcgaccg gcggcgcctc ttccaggttg
     2041 ttggcgggca ggaagctgag caggaagcgg acgtcgtcga ggcaggcctt ctcgtcg
//
LOCUS       DAORNF010000628         2092 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_39551_length_2092_cov_1.233186, whole genome shotgun sequence.
ACCESSION   DAORNF010000628 DAORNF010000000
VERSION     DAORNF010000628.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39551_length_2092_cov_1.233186"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            62..>285
                     /locus_tag="PLV68_19040"
     CDS             62..>285
                     /locus_tag="PLV68_19040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRE03400.1"
                     /translation="MMTQEEFMDVLAMKRAGMTFVEIGALTGYHPATISKWCREGGPP
                     AARTIGDEERVVDPRWADRLTALLVGNSSL"
     assembly_gap    286..445
                     /estimated_length=160
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            613..>1023
                     /locus_tag="PLV68_19045"
     CDS             613..>1023
                     /locus_tag="PLV68_19045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03401.1"
                     /translation="MCRTDRMGALGQSQGRRFKLHPPTLEFARHHGTEIKACQAGDAK
                     RKGKIERPFRDLSESFLEELVVTGVPDDIDALNARARVWVGERVHDRLHRTTGVAPAD
                     RFVVEHGFLKSLPARRFDTDYVEARRVHRALPFIE"
     assembly_gap    1024..1033
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1034..1442
                     /locus_tag="PLV68_19050"
     CDS             <1034..1442
                     /locus_tag="PLV68_19050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03402.1"
                     /translation="RPSDTSSAYSVPADCLGQLVEVRKPVDSNEISIRWADRQIVTHR
                     VPTDGRTEVWDPMHRAQAEASALASTGRRHLHVVRDHDPEPAPTAGPVGRIELPGGDF
                     DVATPDLAARYDHRDLTVNALAADALAVEGGEF"
     gene            1442..>1697
                     /locus_tag="PLV68_19055"
     CDS             1442..>1697
                     /locus_tag="PLV68_19055"
                     /inference="COORDINATES: protein motif:HMM:NF013828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRE03403.1"
                     /translation="MTGGIYEQIKDDLGYLQMDRAGEVFAVLGEQARTEGWSHVEFLA
                     RLIAEQADATRNRRLTARLRYARFPFRKTIDEFDFAFQPTV"
     assembly_gap    1698..1780
                     /estimated_length=83
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1781..>2092
                     /locus_tag="PLV68_19060"
     CDS             <1781..>2092
                     /locus_tag="PLV68_19060"
                     /inference="COORDINATES: protein motif:HMM:NF013828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRE03404.1"
                     /translation="KTHLAVAVAILAVEAGFRGYFTNAEEMVANIAQANREGTLASKL
                     KTYTAPSVLVIDDVGLLPMDRAAASAFYQVVNLRYEKQHSTIVTTNRGLPDWGEIFGD
                     TV"
BASE COUNT          311 a          609 c          601 g          318 t
ORIGIN      
        1 gaggggtggc ccctccagca tgtcgagcta ccacgcaaga caactggagg gaccgcttct
       61 catgatgact caggaggagt tcatggatgt gctcgcgatg aaacgcgctg gcatgacgtt
      121 cgtggagatc ggcgcgctga cggggtatca cccggcgacg atctcgaagt ggtgtcggga
      181 gggtggcccg ccggcggccc gcacgatcgg cgacgaggag cgtgtggtcg accctcggtg
      241 ggctgaccgg ttgacggcgt tgttggtcgg gaactcgtcg ttgttnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnngctcg gcgtggtcgc agcgtgtcgg gctcggcccg
      481 gtgttgtggt gcttcgggat gatcttgtgt tggtcgcgtt ggcggctgtg gtggttcacc
      541 acctcggtcg acagggagca cacgttcgag gggatggttc ggttcttcga cgctgccggt
      601 ggggtcccgc agttgtgtcg cacggacagg atgggagcgc tcggtcagtc gcaggggcgc
      661 cggttcaagt tgcatccacc gacgctcgag ttcgcccgcc atcacggcac cgagatcaag
      721 gcgtgtcagg ccggcgacgc gaaacgcaag ggcaagatcg agcgcccgtt ccgtgacctg
      781 tccgagtcgt tcctcgaaga gctcgtcgtc actggcgtcc ccgacgacat cgacgcgttg
      841 aacgcacggg cacgtgtgtg ggtcggtgaa cgcgtgcatg accggctgca tcgcacgacc
      901 ggtgtcgcgc cggcggaccg gttcgttgtc gagcacgggt tcttgaagtc gttgccggca
      961 cgccggttcg acaccgacta cgtcgaggcc cgccgggtgc atcgggcgtt gccgttcatc
     1021 gagnnnnnnn nnncagacca tctgacacct cctccgctta ctcggtccca gcggactgcc
     1081 tcggtcagct cgtcgaggtc cgcaaacccg ttgacagcaa cgagatcagc atccgatggg
     1141 ccgaccggca gatcgtcact catcgcgttc cgaccgacgg acgcaccgag gtctgggacc
     1201 cgatgcatcg agcacaagcg gaagcgtccg cgttggcgag caccggccgc cggcacctcc
     1261 acgtcgtccg cgaccacgac cccgaaccgg caccgactgc cgggccggtc ggtcggatcg
     1321 agttgcccgg cggcgacttc gatgtcgcca cccccgacct cgctgcccgc tacgaccacc
     1381 gtgatctcac cgtcaacgcg ctcgctgccg acgcgctcgc tgtcgaggga ggcgagttct
     1441 gatgaccggt ggcatctacg aacagatcaa agacgatctc ggctatctgc agatggaccg
     1501 ggctggtgag gtgttcgctg tcctcggtga acaggcccgc accgagggct ggtctcacgt
     1561 cgagttcctc gcccggctca tcgccgagca agccgacgcg acccgcaacc ggcgactcac
     1621 cgcacgactc agatacgcgc ggttcccgtt ccgcaagacc atcgacgagt tcgacttcgc
     1681 gttccaaccg accgtcgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn aagacacacc tcgccgtcgc
     1801 cgtcgcgatc ctcgctgtcg aggccggctt ccgcggctac ttcaccaacg ccgaagagat
     1861 ggtcgcgaac atcgcccaag ccaaccggga aggcaccctc gcgtcgaaac tgaagaccta
     1921 caccgcacca tcagtgctcg tgatcgatga cgtcggcctg ctccccatgg accgggccgc
     1981 cgcgtcagcg ttctaccaag tcgtcaacct gcgctacgag aaacagcact cgacgatcgt
     2041 cacgaccaac cgcgggctgc ccgactgggg cgagatcttc ggcgacaccg tc
//
LOCUS       DAORNF010000629         2091 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_39562_length_2091_cov_1.646857, whole genome shotgun sequence.
ACCESSION   DAORNF010000629 DAORNF010000000
VERSION     DAORNF010000629.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39562_length_2091_cov_1.646857"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..775)
                     /locus_tag="PLV68_19065"
     CDS             complement(<1..775)
                     /locus_tag="PLV68_19065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRE03405.1"
                     /translation="MSELDTAELVASTPTDGDSGDATATYPVDTPADHGVDAPPPVIE
                     LREVSREFDADPPVRAMNHVNLLIEQGDYVAIIGPSGSGKSTLLNTLGLLDRPTSGTY
                     LLDGIDTSELNDRERAALRSDRIGFVFQSFHLLAHRSALENVMIGSLYQGISRRERRR
                     RAVEALDRVGLAKRAGFLPSRMSGGERQRVAVARALAARPSLLLCDEPTGNLDSATTV
                     AVLDLFDELLADESVVHELAAAHPALDGQQLRSLRRAALL"
     gene            complement(792..>1299)
                     /locus_tag="PLV68_19070"
     CDS             complement(792..>1299)
                     /locus_tag="PLV68_19070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03406.1"
                     /translation="VAAGAAVTIDVRDYGVQLPGTVTYVGKPASSDASSSSGRLQVIV
                     EADDPSALARYVYSSVRIIVDVASTGGEVLTVPIAAVTLGPDGDSRIEIERAPITDRD
                     LGSTEFVSVEVGLSASGMVQISSATGAPPIEVGDRVVLGLDSGSRLNRDDSSSGASSD
                     SVASSAQG"
     assembly_gap    1300..1361
                     /estimated_length=62
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1362..>1673)
                     /locus_tag="PLV68_19075"
     CDS             complement(<1362..>1673)
                     /locus_tag="PLV68_19075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03407.1"
                     /translation="AATSAEDNRVSTAEDGAAAVKQAQQAKALANAALADATKPRDTT
                     TLEQTLADAKTALDQARADYAEADASIGTKVPSGEIVFIPVLPSTVTDVSAALGNPAN
                     D"
     assembly_gap    1674..1699
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1700..>2091)
                     /locus_tag="PLV68_19080"
     CDS             complement(<1700..>2091)
                     /locus_tag="PLV68_19080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03408.1"
                     /translation="SIGPSRTDQDQLRTLRQAITAAEANVLAAQQTLDEAKRGPSGAE
                     LLQLQQSLSVATDQVPIAQGRADRNNRAAVAQVQSAIANRDSAIRQRDAAKAAADAAK
                     VPGAIDPNTGEAYTAQAIAALDNAVADA"
BASE COUNT          272 a          693 c          710 g          328 t
ORIGIN      
        1 cgagcaaggc tgcccgtcgt aaagagcgca actgctgacc gtcgagcgcg ggatgtgccg
       61 cggccagctc gtgaacgacg ctttcatcgg ccagcagctc gtcgaacagg tcgagcacgg
      121 ccacggtcgt ggccgagtcg aggttgccgg tcggctcgtc gcacaacagc aggctcggcc
      181 gggccgcgag agcacgggcc accgccaccc gttggcgctc gccaccgctc atccggctgg
      241 gaaggaaccc ggcccgcttg gccaagccga cgcggtcgag cgcctcgacg gctcgccgcc
      301 gccgctcgcg gcgggagatg ccctgataca gactgccgat catcacgttc tcgagcgcgc
      361 tgcggtgcgc cagcaggtgg aacgactgga acacgaaccc gatccggtcg gagcgcagcg
      421 cggcccgttc gcggtcgttg agctcactgg tatcgatgcc gtcgagtagg taggtacccg
      481 acgtcggccg atcgagcaga ccgagggtgt tcagcaacgt cgacttaccc gagcccgacg
      541 ggccgatgat cgccacgtag tcgccttgct cgatcaacag gttgacgtgg ttcatcgccc
      601 gcaccggtgg atcggcgtcg aactcgcggc tgacctcgcg gagctcgatg accggcggcg
      661 gcgcgtcgac gccgtggtca gctggggtgt cgacggggta ggtggcggtg gcgtcgccgc
      721 tgtcgccatc cgtcggcgtc gacgcgacca gttcagccgt gtccagttcg ctcatccgtc
      781 gtcagccttg ctcagccctg ggcgctcgac gccaccgagt cgctgctggc cccgctgctg
      841 ctgtcgtcgc ggttgagccg cgaacccgag tccaggccga gcaccacccg atcgccgacc
      901 tcgatcgggg gcgccccggt cgcggaagag atctgcacca tgcccgacgc gctgagcccg
      961 acctcgacgc tgacgaactc ggtgctgccc agatcgcgat cggtgatcgg cgctcgctcg
     1021 atctcgatcc ggctgtcgcc gtcgggaccg agcgtgaccg cggcgatcgg caccgtcagc
     1081 acctcgccgc cggtggaggc gacgtcgacg atgatccgca ccgacgaata gacgtagcgc
     1141 gccaacgccg acggatcgtc ggcttcgacg atcacctgca accgccccga gctggagctg
     1201 gcgtcgctgg acgccggctt gccgacgtag gtgaccgtgc cgggaagctg taccccgtag
     1261 tcgcggacgt cgatcgtgac ggctgcacct gccgcgaccn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncgtcgttgg ccggattgcc
     1381 gagggctgcc gacacgtcgg tcaccgtcga cggcaacacc gggatgaaca cgatctcgcc
     1441 cgagggaacc ttggtgccga tcgaggcgtc ggcctcggcg tagtcggccc gggcctggtc
     1501 gagcgccgtc ttggcgtccg ccagggtttg ctcgagcgtg gtggtgtcgc gcggcttggt
     1561 ggcgtcggcg agtgcggcgt tggccagcgc cttggcctgc tgggcctgct tcacggcagc
     1621 ggcgccgtcc tcggcagtcg acacgcggtt gtcctcggcg ctggtggcgg cgannnnnnn
     1681 nnnnnnnnnn nnnnnnnnnt cggcgtcggc cacggcgttg tcgagcgcgg cgatcgcctg
     1741 ggcggtgtac gcctcgcccg tgttggggtc gatcgcaccg ggcaccttgg ccgcatcggc
     1801 agcagccttg gccgcgtcac gctgacggat cgcgctgtca cggttggcaa tcgcgctctg
     1861 cacctgggcg accgcagccc ggttgttccg atcggcacga ccttgggcga tcggcacctg
     1921 atcggtcgcc acgctgagcg attgttgcag ctgcaagagc tccgctccgg acggtccgcg
     1981 cttggcctcg tcgagcgttt gctgtgccgc cagcacgttg gcctcggccg ccgtgatcgc
     2041 ctgccgcagc gtgcgcagct ggtcctgatc ggtgcgcgac ggcccgatgc t
//
LOCUS       DAORNF010000630         2089 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_39606_length_2089_cov_1.647001, whole genome shotgun sequence.
ACCESSION   DAORNF010000630 DAORNF010000000
VERSION     DAORNF010000630.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39606_length_2089_cov_1.647001"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..796
                     /locus_tag="PLV68_19085"
     CDS             <1..796
                     /locus_tag="PLV68_19085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03409.1"
                     /translation="SAAGQPASLATAWVNLAAVAIVSNDLPVALAYYARAEPALRDLD
                     QHTVLVPLYNNRAQVREATGDVPGAIADLQAGGRSAAVVGALAQSLAMSTRAVQLLYA
                     TGRQAESEPLLEQVADVARALGDRAALQRALGDRALLVMARGDVAAAARLLDEQEAIC
                     TATGDAAGLAAAVGNRAILQRTLGDLPGALASIERQLEIARASGNGQAYLFATANRGE
                     LLGAMGRVGEGLAALAEARAMAVANGLTPMVEELDRMAAAIRATQQ"
     assembly_gap    894..978
                     /estimated_length=85
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <979..2030
                     /locus_tag="PLV68_19090"
     CDS             <979..2030
                     /locus_tag="PLV68_19090"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRE03410.1"
                     /translation="ALCVIRDGEVVVDLWGGYADEARTRSWTADTLVNVYSVGKALAA
                     TMLLQLVDAGALTLDTRVCEVWPEFAAGGKAAVTVRQALCHEAGVPAIRQTLTDDDLR
                     DWTTMADALAATETWWPIGTRHAYHTNTYGFLLGEIVRRVTGDGPGRRLQRVAADVGD
                     VPADVFFGVPDTDQARCADVIWAPSRPMDPHAIDRSQLTGDALMNVLAHFNPPGASSI
                     GLVNTPSWRSAEVPSTNGHGSARGVARFYAALADPDRLLSPSLLHTATRPQTLGPCTI
                     LGEEIAFGLGFTPTTERRPLGPNPNSFGHFGTGGALGFADPDSGVAFGYVMNHVIPRW
                     QSTRNRALIDALFAG"
BASE COUNT          298 a          715 c          693 g          298 t
ORIGIN      
        1 gtcggctgct ggccaacccg cgtcacttgc gaccgcgtgg gtcaacctcg ccgcggtcgc
       61 gatcgtcagc aacgacctgc ccgtagcgct cgcgtactac gcccgtgccg agccggcgtt
      121 gcgcgacctg gaccagcaca ccgtgctcgt gccgctgtac aacaaccggg cgcaggtgcg
      181 cgaggccaca ggcgacgtcc ccggggcgat cgccgacctg caggcgggtg gacgatctgc
      241 agccgtcgtc ggcgcgctcg cacagagctt ggccatgtcg acccgagcgg tgcagttgct
      301 gtacgcaacc ggacgtcagg ccgagagcga acccctcctg gagcaggtcg ccgacgtggc
      361 ccgggcgctg ggcgaccggg ccgcgctgca gcgtgcactc ggcgatcggg cgctgctggt
      421 gatggcccgc ggcgacgtcg ctgcagcggc gcgattgctg gacgaacagg aggcgatctg
      481 caccgcgacc ggtgacgcgg cgggtcttgc cgcggcggtc ggcaaccggg cgatcctgca
      541 gcgcactctg ggtgatctgc ccggcgcgct ggcttcgatc gagcggcagt tggagatcgc
      601 ccgcgcatcc ggcaacggcc aggcctacct gttcgccaca gcgaaccgtg gcgagttgtt
      661 gggagcgatg ggccgagtcg gcgaaggcct cgccgcgctc gccgaagccc gggcgatggc
      721 tgtcgccaac ggcctcaccc cgatggtcga agagctcgac cgcatggccg ccgccatccg
      781 cgccacccag cagtagtggc cacgtcagcg cagcgagtgc cagcgaagcg catactggag
      841 caatggcgga cgacagtgac cccagcatcg ccgagccggt tgttgccgag ccgnnnnnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      961 nnnnnnnnnn nnnnnnnnca gcgctgtgcg tcatccgcga cggcgaggtc gtggtcgacc
     1021 tgtggggtgg ttacgccgac gaggcgcgca ccaggagttg gacggccgac acgctggtga
     1081 acgtctactc ggtcggcaag gcgctggccg cgaccatgct gttgcagttg gtcgacgcgg
     1141 gtgcgctcac cctcgacacc cgtgtctgcg aggtgtggcc agagttcgcc gcgggtggca
     1201 aggccgcggt caccgttcgt caggcgctct gtcacgaagc tggtgtgccg gccatccgcc
     1261 agacgctcac cgacgacgat ctgcgcgact ggacgacgat ggccgacgcc ctggcggcga
     1321 ccgagacgtg gtggccgatc ggcacccgcc acgcctacca caccaacacc tacggctttc
     1381 tactcggtga gatcgtgcgc cgcgtgactg gcgacgggcc gggccgacgg ctgcagcgcg
     1441 tcgccgccga cgtcggtgac gttcctgccg acgtgttctt cggcgtaccc gacaccgatc
     1501 aggcccgctg cgccgacgtg atctgggccc cgagccgacc gatggatccg cacgcgatcg
     1561 accgctccca actcacgggt gacgcgctga tgaacgtgct cgcccacttc aatccgcccg
     1621 gcgcatcgtc gatcggtctc gtcaacacac cgagttggcg cagtgccgag gtgccctcca
     1681 ccaacggtca cggcagcgca cgcggtgtgg cccggttcta cgccgcgctg gccgaccccg
     1741 atcgcctcct gtcgccgtcg ctgttgcaca ccgccacccg tccgcaaacg ctggggccat
     1801 gcacgatcct gggtgaggag atcgcgttcg ggctcgggtt cacccccacc accgagcgcc
     1861 gtccgctcgg tccgaacccc aacagcttcg ggcacttcgg cacgggcggt gcgctgggct
     1921 ttgccgaccc cgacagcgga gtcgcgttcg gctatgtcat gaaccacgtc attccccgct
     1981 ggcagagcac ccgcaaccga gccctgatcg acgcgctgtt cgcgggctga gcgcagccct
     2041 tgtgcgagga tgcggccatg cgacgctcgg gctggccgtc gacgacagg
//
LOCUS       DAORNF010000631         2088 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_39626_length_2088_cov_1.666011, whole genome shotgun sequence.
ACCESSION   DAORNF010000631 DAORNF010000000
VERSION     DAORNF010000631.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39626_length_2088_cov_1.666011"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="PLV68_19095"
     CDS             complement(<1..201)
                     /locus_tag="PLV68_19095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AhpC-related (seleno)protein"
                     /protein_id="HRE03411.1"
                     /translation="MVDTAMVDTAMVDTATIDTATIDATTVDAGTVDAAAPGIAASIA
                     DVVVPDSPAALEPPAAASALPPQ"
     assembly_gap    239..388
                     /estimated_length=150
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<389..1870)
                     /locus_tag="PLV68_19100"
     CDS             complement(<389..1870)
                     /locus_tag="PLV68_19100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRE03412.1"
                     /translation="MNVLIAVPLVALVLLAAVGFTSLQRASVRGDEYQQLKVAQDLSQ
                     DLVAPRANLIAAWSSVAKIGVLVASVQTTRTRGEIRSELENLDKARTAYFAAVDYWRG
                     QDLPDIQRKAFENSAKSGENFFDKVDSLIVPKMQAEGDPDPSVVLSAVRAIEYDYTIQ
                     QVFLSELQRDVVADVNAREQSTDRFVGIVQLLIIGAVAALLLIVLVVAFAVRRSIVAP
                     ILALTDQARRAADHELPEAVRQIQNQPADLPLPEVAAFHTETRDELADLGSSFTSVQS
                     AALSLAAEQAQARRIVSENLIAIARRTQSLLGRSLSSLSEMESNERDPEALARLFRLD
                     HLTTRMRRNAQSLLVLADAEQQRLWSPPVAIGDVVRAALSEIEHYNRVDLGDLGDHGK
                     VMVQGALAADVAHLLAELLENATSFSSPTTQVMVVGRTVGNGHQLAIVDYGIGMSEDE
                     LAAANVSLRRQADFGTESSKVLGFQVVARIAARLGVQVVLANTA"
     gene            1875..>2088
                     /locus_tag="PLV68_19105"
     CDS             1875..>2088
                     /locus_tag="PLV68_19105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03413.1"
                     /translation="MPIFNFRNIGPAFVVCDPCRGLYRQRVVALERFVSLRSARGDRL
                     ESLKSRAVVPMRWRALATIRAPAMSGD"
BASE COUNT          323 a          644 c          655 g          316 t
ORIGIN      
        1 ctgcggcggc aacgctgacg cagcggccgg gggctccagt gccgctggac tgtccgggac
       61 gacgacgtcc gcgatggatg cggcgatacc gggagccgct gcatcaacgg ttccggcatc
      121 aacggttgtg gcatcaatgg tcgcggtgtc aatggtcgcg gtgtcgacca tcgcggtgtc
      181 caccatcgcg gtgtcgacca tcgcggtgtc gggagtgcca ggacgatcga gcacaccgnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnncg ccgtgttggc cagcaccacc tgtacgccca
      421 gacgagccgc gatgcgggcg accacctgga agccgagcac cttgctcgac tcggtgccga
      481 agtccgcctg acgacgcagg ctgacgttgg ccgccgccaa ctcgtcctcg ctcatgccga
      541 tgccgtagtc gacgatggcc agctggtgac cgttgcccac ggtgcgaccc accaccatca
      601 cctgggtggt cggcgacgag aagctggtcg cgttctccaa gagctcggcc aacaggtggg
      661 cgacatccgc ggcgagggcg ccctgcacca tcaccttgcc gtgatcgccg aggtcgccga
      721 ggtcgactcg gttgtagtgc tcgatctccg acaacgcggc gcggaccacg tcaccgatgg
      781 ccaccggcgg cgaccacagc cgctgctgtt cggcgtcggc caacaccagc agcgactggg
      841 cgttgcggcg catgcgggtg gtgaggtggt cgaggcggaa cagccgggcc aacgcctcgg
      901 gatcgcgctc gttcgactcc atctccgaca gcgacgacag cgatcgtccg agcaggctct
      961 gcgtccgccg ggcgatggcg atcaggtttt cggacacgat ccggcgggcc tgggcctgct
     1021 cggcagccaa cgacaatgct gccgactgca ccgacgtgaa gctgctgccg agatcggcaa
     1081 gttcgtcgcg ggtctcggtg tggaaggccg cgacctctgg cagcgggagg tccgccggct
     1141 ggttctggat ctggcgcacc gcctcgggca gttcgtggtc ggcagcacgg cgggcctgat
     1201 cggtcagcgc caagatcggg gcgacgatgc tgcgccgcac ggcgaaggcc accaccaaca
     1261 cgatcagcaa cagggccgcg acggcgccga tgatcaacag ctgcacgatg ccgacgaagc
     1321 ggtcggtgct ctgctcacga gcgttcacgt cggcgaccac gtcgcgctgc aactcgctga
     1381 ggaacacctg ctggatcgtg tagtcgtact cgatcgcgcg cacggcactg agcaccaccg
     1441 acggatcggg atcaccttcg gcctgcatct tcggcacgat cagcgagtcg accttgtcga
     1501 agaagttctc acccgacttg gcgctgttct cgaacgcctt gcgttggatg tcgggcaggt
     1561 cctgcccgcg ccagtagtcg accgcggcga agtaggcggt gcgtgccttg tccaggttct
     1621 ccaactcgga gcgaatctca ccccgggtgc gcgtggtctg taccgacgcg acgaggacac
     1681 cgatcttggc gaccgacgac cacgcggcga tcaggttggc acgcggtgcc accaggtcct
     1741 ggctcaggtc ttgggcgacc ttcaactgct gatactcgtc gccgcgcacg ctggcccgct
     1801 gcagcgaggt gaagcccacc gctgccaaca acaccagtgc caccaacggc acagcgatca
     1861 gcacgttcac ccgtgtgccg atcttcaact tccgcaacat aggccccgcc ttcgtcgtct
     1921 gcgatccctg caggggtctg tatcggcagc gggtggtggc gcttgagaga ttcgtgagcc
     1981 tgagatcggc gcggggcgac cgtctcgaat ctctcaagag ccgcgctgtg gtgccgatga
     2041 ggtggcgtgc actcgcaacg atcagggcgc cggcgatgag cggcgacg
//
LOCUS       DAORNF010000632         2088 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_39627_length_2088_cov_1.653714, whole genome shotgun sequence.
ACCESSION   DAORNF010000632 DAORNF010000000
VERSION     DAORNF010000632.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39627_length_2088_cov_1.653714"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="PLV68_19110"
     CDS             complement(<1..299)
                     /locus_tag="PLV68_19110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HRE03414.1"
                     /translation="MLAAGGTIAAYVAVQIIDATAPIVNRIGSTEATIVPVRSVSVGD
                     YVWVDTNRDGRQGNPATEPGIPGVVLVLVGPDGEPVVDVFGNPVGPTTTGPNGEYT"
     gene            complement(766..>1027)
                     /locus_tag="PLV68_19115"
     CDS             complement(766..>1027)
                     /locus_tag="PLV68_19115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03415.1"
                     /translation="DPGETVTMTYTFRVEANQGIDMRNSTIVDHIDNSIFDLGDPPTA
                     DTTGSTYDGQALAAGDFELSINADGDLVIELSTSGPVARTSC"
     assembly_gap    1028..1059
                     /estimated_length=32
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1060..1986)
                     /locus_tag="PLV68_19120"
     CDS             complement(<1060..1986)
                     /locus_tag="PLV68_19120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03416.1"
                     /translation="MPAGLSYGPVSFNAQLTTWDANGLNQTTSAFSGFTPAFSGNGFT
                     STVDLPEKSVLTITYTASVQDPEPLRALLQAVHNARNGEPGNFEINLVNTATFGGNVD
                     RTATVRMRGSIPAAPGPNIGNAFSKTSDWSSRNVVTDEDDNLVPPQDITYTLKVDLGQ
                     WDNSGGNFSLARNVVISDDLPAGASWNTSDLEFIKIASGSGITLSLLTACPADQVAME
                     SQPVGSYCVNGQRLLINVGQNSATNLTIAVKAKVNSLDGLTANQGTTPIVGATPYRLR
                     NDALYYYRAGDPYTARRDVTVVNELVTAHKKAR"
BASE COUNT          285 a          630 c          739 g          402 t
ORIGIN      
        1 gtgtactcgc cgttgggacc cgtcgtggtc gggccgaccg ggttgccgaa cacgtcgacc
       61 accggctcac cgtccgggcc gacgagcacc aacacgacgc cggggatgcc cggctcggtg
      121 gcggggttgc cctggcggcc gtcgcggttg gtgtcgaccc acacgtagtc accgaccgac
      181 accgaacgca cgggtacgat cgttgcttcc gtgctgccga tgcggttgac gatcggagcc
      241 gtcgcatcga tgatctgcac cgcgacgtaa gccgcgatcg tgccaccggc cgcgagcacg
      301 gtgctcgcct tctggcgcag cgtgaccgtg ccggtgccgc cgtcgtagac cgcttcgagg
      361 ttgccgccga tgtcgaccgg gccggcaacc cggccgctgt agccgcgttg gctgcggtga
      421 cgaagccgag gaaggtcgcc gccatcggca gcacgtcgtc caccggctgg atcgccacgc
      481 cgttgtagtt gccgtggggg atgaactcga tccggtagac gtaggtgtcc tggacgagat
      541 tgccctcgcc gtccatctgc gccttcaggg tctcgaccca gtcctcgctg tcgcggtcgt
      601 agacgcgctt gcgcacttcg gcctcgtcgc cgtacgacgt ggcttcggtc ttcgactgcg
      661 accagtacag cggttcgtcg tcggcaccga acaaggtggc gcggttggtg atcgtcttgg
      721 tgaccttgcc gtcgaacggc atcgtctcca gcgtcagctt gacgatcaac aacttgtccg
      781 cgccacgggt cccgacgtgc tcagctcgat cacgaggtcg ccgtcggcgt tgatgctcag
      841 ctcgaagtcc ccagccgcga gcgcctggcc gtcgtaggtg ctgccggtgg tgtccgccgt
      901 cggcgggtcg ccgaggtcga agatgctgtt gtcgatgtgg tcgacgatgg tgctgttgcg
      961 catgtcgatg ccctggttgg cttcgacccg gaacgtgtag gtcatcgtca cggtctcgcc
     1021 gggatcgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng cgcgctttct tgtgtgcggt
     1081 gactagctcg ttgaccaccg tcacgtcacg gcgtgcggtg taggggtcgc ccgcgcggta
     1141 gtagtacagc gcatcgttgc gcaggcggta cggggtggcg ccgacgatcg gcgtggtgcc
     1201 ctggttcgcc gtgagaccat ccaacgagtt gaccttcgcc ttgacggcga tcgtgaggtt
     1261 ggtggcgctg ttctgaccga cgttgatcag cagccgctgg ccattgacgc agtacgaccc
     1321 gaccggttgc gattccatcg ccacctggtc ggcggggcac gccgtcagca ggctcagcgt
     1381 gatccccgaa cccgaggcga tcttgatgaa ttccagatcg gaggtgttcc aactcgcccc
     1441 ggcgggcagg tcgtcgctga tgacgacgtt gcgggccagg ctgaagttgc cgccgctgtt
     1501 gtcccactgg cccaggtcga ccttgagggt gtaggtgatg tcctgcggcg ggacaaggtt
     1561 gtcgtcctcg tcggtgacga cgttgcggga gctccagtcg gacgtcttgg agaaggcgtt
     1621 gccgatattg gggccggggg cggcgggaat cgagccgcgc atccgcacgg tggccgttct
     1681 gtcgacgttg ccgccgaagg tggccgtgtt gacgaggttg atctcgaagt tgccaggctc
     1741 gccgttgcga gcgttgtgca ccgcttggag cagggctcgg agtggctcgg ggtcttgcac
     1801 gctggcggtg taggtgatcg tcagcaccga cttttcgggg aggtcgacgg tgctggtgaa
     1861 gccgttaccc gagaaggcgg gggtgaaccc agagaacgcg ctggtcgtct ggttgaggcc
     1921 gttggcgtcc cacgtggtga gctgcgcgtt gaacgagacg gggccgtagc tcagcccggc
     1981 gggcaagcgg tcggcgatgg tgtacccggt acgcgccgtc ggcgagttga cccgcagcgt
     2041 gtacgtgatc ggcacgccga tcaccgtcgc gttcagcgtg accgtgcc
//
LOCUS       DAORNF010000633         2082 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_39743_length_2082_cov_1.830784, whole genome shotgun sequence.
ACCESSION   DAORNF010000633 DAORNF010000000
VERSION     DAORNF010000633.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39743_length_2082_cov_1.830784"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="PLV68_19125"
     CDS             <1..495
                     /locus_tag="PLV68_19125"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HRE03417.1"
                     /translation="PATALSLLVDRSGSMGGKPLAASAVAAAAVASRSPEDYSVLAFG
                     KDVVVAKSQDAFKESERVVSDVLTLRGFGTTDLAGALTVAHRQLMRSRAGRRIAVLLS
                     DCRATVDGDVAAAAGALDELVIIAPAGDSAEAEKLAAATGARLTTVTGPSEVATAINL
                     LLDA"
     gene            complement(514..>1174)
                     /locus_tag="PLV68_19130"
     CDS             complement(514..>1174)
                     /locus_tag="PLV68_19130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HRE03418.1"
                     /translation="IKIEPDVPLDRACLLGCGVVTGWGSSVYAAQVSPGDTVAVVGCG
                     GIGSNAIQGARLAGAKRIIAIDPIEFKREKAMEFGATHTAASMAEALPLIQELSWGTM
                     ANKVIMTMGVGSGEVMGEALALTAKRGRVVVTNIHPALEMGASVSLLDLTLMEKQVIG
                     SLFGSGNPRADIPKLLSLWREGQIDLDGLVTKTLTLDQVNEGYDAMREGTTIRSVIKF
                     D"
     assembly_gap    1175..1316
                     /estimated_length=142
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1317..1627)
                     /locus_tag="PLV68_19135"
     CDS             complement(<1317..1627)
                     /locus_tag="PLV68_19135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HRE03419.1"
                     /translation="MKTRAAVLWEIGAEWSVEEIDLDPPGPGEVLVKMAASGLCHSDE
                     HLVTGDLPFALPIVGGHEGSGVVQQVGPGVSWLAPGDHVVFGFIPSCGRCPSCSTGHS
                     NL"
     gene            complement(1684..>2082)
                     /locus_tag="PLV68_19140"
     CDS             complement(1684..>2082)
                     /locus_tag="PLV68_19140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002518291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HRE03420.1"
                     /translation="LADADLADAELADADLAAAVAAEVQIARHRHPLTGVVAPLRLHA
                     AIGAALAAVGLAAVDRVQDVQRDEIPLFGPEAVKGLEFDATVVVNPTEILDGTARGAR
                     LLYVAMTRSVQVLRMVGDAPLDTTVFGAAR"
BASE COUNT          296 a          689 c          656 g          299 t
ORIGIN      
        1 ccggcgacgg cgctgtcgct gttggtcgac cgcagcgggt cgatgggcgg caagcccctc
       61 gccgcgtcgg ccgtcgccgc agccgccgta gccagccggt cgcccgagga ctacagcgtg
      121 ttggccttcg gcaaggacgt cgtcgtcgcc aagtcgcagg acgcgttcaa agagagcgag
      181 cgggtcgtct ccgacgtgtt gaccctgcgc ggtttcggca ccaccgactt ggccggggcg
      241 ctcaccgtcg cccatcgcca actgatgcgg tcacgcgccg gacgccggat cgcggtgctg
      301 ctcagcgatt gccgcgccac cgtcgacggc gacgtcgccg cggcggccgg cgcgctggat
      361 gagttggtga tcatcgcccc cgctggcgac tcggccgaag ccgaaaagct ggccgccgcg
      421 accggggcgc ggctcaccac tgtgaccggc ccgagcgagg tcgcgaccgc gatcaacctc
      481 ctcctcgacg cgtgatcgcg tgatcgcgat ggttcagtcg aatttgatga ccgagcggat
      541 ggtggtgcct tcgcgcatcg cgtcgtagcc ctcgttgacc tggtcgaggg tgagggtctt
      601 ggtgaccagg ccgtcgaggt cgatctgacc ctcgcgccac aggctgagca gcttgggaat
      661 gtcggcgcgc gggttgcccg aaccgaacag cgaaccgatg acctgcttct ccatcaacgt
      721 caggtcgagc aggctgacac tggcccccat ctcgagcgcc gggtgaatgt tggtgaccac
      781 gaccctgccc cgcttggcgg tgagtgcgag cgcctcgccc atcacctcgc ccgagccgac
      841 gcccatggtc atgatgacct tgttggccat ggtgccccag ctcagctcct ggatcagcgg
      901 cagcgcctcg gccatgctgg ccgcggtgtg ggtggcaccg aactccatcg ccttctcgcg
      961 cttgaactcg atcgggtcaa tggcgatgat ccgcttggcg cccgcgagtc gcgccccctg
     1021 gatggcgttg gagccgatgc caccgcagcc gaccacggcc acggtgtcgc ccgggctgac
     1081 ctgagccgcg tagacgctgc tcccccagcc ggtgaccacg ccgcagccga gcaggcaggc
     1141 tcgatcgagg gggacgtcgg gttcgatctt gatgnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnaggt
     1321 tggagtggcc cgtcgaacag ctcgggcagc gaccacagga cgggatgaac ccgaacacca
     1381 cgtggtcacc cggggccagc cagctcacgc ccgggccgac ctgttgcacc acgccagaac
     1441 cctcgtgacc gccgacgatc ggcagtgcga agggcaggtc gccggtgacg agatgctcgt
     1501 cgctgtgaca caggccggac gctgccatct tcaccaacac ttcgccgggg ccgggcggat
     1561 cgagatcgat ctcttccacg ctccactcgg caccgatctc ccacaggacg gctgctctgg
     1621 tcttcatctc gtgacccccg gtcgatgtgt cggccggtac tgcaccggcg tctggacgcg
     1681 aacctaccgc gctgcgccga acaccgttgt gtcgagcggg gcgtcaccga ccatgcgcag
     1741 cacctgcacc gagcgagtca tcgcgacgta caacagtcgg gcgccgcggg cggtgccgtc
     1801 gaggatctcg gtcgggttga ccacgacggt cgcgtcgaac tccaacccct tcaccgcttc
     1861 ggggccgaac agcgggatct cgtcgcgctg cacgtcctgc acgcggtcga ccgcggccaa
     1921 gcccacggcc gcgagcgcgg cgccgatcgc cgcatgcagg cgcagcggtg caaccacgcc
     1981 agtcaacggg tggcggtggc gggcgatctg gacctcggcc gcgaccgcag cagccaggtc
     2041 cgcatcggcc agctccgcat cggccaggtc cgcatcggcc ag
//
LOCUS       DAORNF010000634         2078 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_39851_length_2078_cov_1.827484, whole genome shotgun sequence.
ACCESSION   DAORNF010000634 DAORNF010000000
VERSION     DAORNF010000634.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39851_length_2078_cov_1.827484"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>210
                     /locus_tag="PLV68_19145"
     CDS             <1..>210
                     /locus_tag="PLV68_19145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03421.1"
                     /translation="QGSWFGALPADLRGQLDVVVSNPPYIAEGDPAVAASVTEHEPHL
                     ALFSGDDGLDAIRTIAADAPAWLAP"
     assembly_gap    211..227
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(359..>2078)
                     /locus_tag="PLV68_19150"
     CDS             complement(359..>2078)
                     /locus_tag="PLV68_19150"
                     /inference="COORDINATES: protein motif:HMM:TIGR01701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FdhF/YdeP family oxidoreductase"
                     /protein_id="HRE03422.1"
                     /translation="TIFLIAGMLPLGLALTQTIGIGKGSVHITDFAQADMVVIVGQNP
                     GTNHPRMLSTLEAAKRAGTRIVAINPLKEAGLVRFRNPQKVGGMIGSGTALADDYLQI
                     KINGDLAFFKAVNHLLLAGGDRDIDTDFIARFTTGFDQFAADAANFDWDTYRAATGFE
                     PSVAEAFAQQVRASKSVIVCWAMGLTQHANSVATIREVVNFLLLRGNIGKPGAGVCPV
                     RGHSNVQGDRTMGIYEKPSMAFLDALGAEFGFDPPRRRGTDVVDAIAGMHSGAVRVFI
                     GMGGNFASAAPDTAATLAGLARCSLTVQVSTKLNSSHLAVGDQALILPTKGRTERHQI
                     DGADQTVTVEDSMGVVHASHGRLPPASDDLLSEVQIVAEIARHTLDPQACSADWIGLS
                     RDHAAIRDSISRVIPGFDDYNAKVAHPGGFVLPHPPRDDRSFPTDTGLANFSVNPLTY
                     ATAPAGSLLLQTVRSHDQYNTTIYGLDDRYRGIHGGRRVVFVNPADLTALDLADEQVV
                     DLVTDAADGSTRVAPRFRIVAYPTSLGGCAAYFPETNVLVGLDQQGEAGTPVYKSIPV
                     RLVPSA"
BASE COUNT          314 a          672 c          755 g          320 t
ORIGIN      
        1 tgcaggggtc gtggttcggc gcgctgcccg ccgacttgcg tggccaactc gacgtggtgg
       61 tgtccaaccc gccctacatc gccgagggtg atcccgcggt cgccgcgtcg gtcaccgaac
      121 acgagccgca cctggcgctg ttcagcggtg acgacggact cgacgcgatc cgcaccatcg
      181 ccgccgacgc ccccgcgtgg ctcgcgccgg nnnnnnnnnn nnnnnnngga cagggcgacg
      241 cggtacgcga cctgttgacc gcggccggac tcaccgacgc cgagattcgc cccgacctcg
      301 ccggtcacga ccgcatcgcc atcgcccgca acacccgctg agggggtggg gccggctgtc
      361 aggcgctggg gacgaggcgg acggggatcg acttgtagac aggggtgccg gcttcgcctt
      421 gctggtcgag gccgaccaac acgttggtct ccgggaagta cgccgcacat cccccgaggc
      481 tggtggggta ggcgacgatg cggaagcggg gagcgacccg ggtggaaccg tcggccgcat
      541 cggtaacgag atcgaccacc tgctcgtcgg cgagatcgag cgccgtgaga tcggccgggt
      601 tgacgaacac cacccggcgc ccgccgtgga tgccgcggta gcggtcgtcg agcccgtaga
      661 tcgtggtgtt gtactgatcg tggctgcgca cggtctgcag cagcagcgac ccggcgggcg
      721 cggtggcgta ggtgagcggg ttgaccgaga agttggccag tcccgtatcg gtcgggaacg
      781 agcggtcgtc tcgcggtggg tgcggcaaca cgaagccacc ggggtgggcc accttggcgt
      841 tgtagtcgtc gaagccgggg atcacccggc tgatcgagtc gcggatggcc gcatggtcgc
      901 ggctgagccc gatccagtcg gccgagcagg cctgcggatc gagggtgtgg cgggcgatct
      961 cggccacgat ctgcacctcg ctgagcaggt cgtcggatgc cggaggaaga cggccgtgcg
     1021 aggcgtggac cacgcccatc gagtcctcga ccgtgaccgt ctgatcggcc ccatcgatct
     1081 gatgacgctc ggtgcggcct ttggtgggca ggatcagcgc ctggtcgccg acggcgagat
     1141 gcgacgagtt gagcttggtc gagacctgca ccgtcaggct gcagcgggcc agcccggcca
     1201 gcgtcgcggc cgtgtcgggc gccgccgacg cgaagttgcc gcccatgccg atgaacaccc
     1261 gcaccgcacc gctgtgcatg ccagcgatcg cgtcgaccac atcggtgccg cggcgccggg
     1321 gtgggtcgaa gccgaactcg gcaccgagcg cgtcgagaaa cgccatcgac ggcttttcgt
     1381 agatgcccat cgtgcggtca ccctgcacgt tggagtggcc gcgcaccggg cacaccccgg
     1441 cacccggctt gccgatgttg ccgcgcagca gcaggaagtt gacgacctcg cggatggtgg
     1501 ccaccgagtt ggcgtgttgg gtcagcccca tcgcccagca gacgatgacg ctcttggacg
     1561 cgcggacctg ctgggcgaac gcttccgcga ccgagggctc gaagccggtc gcggcgcgat
     1621 aggtgtccca gtcgaagttg gccgcgtcgg cggcgaactg gtcgaacccg gtggtgaagc
     1681 gggcgatgaa gtcggtgtcg atgtcgcggt cgccaccggc gagcagcagg tggttgaccg
     1741 ccttgaagaa ggccaggtcg ccgttgatct tgatctgcag gtagtcgtcg gccagggcag
     1801 tgcccgagcc gatcatgccg ccgaccttct gcgggttgcg gaaccgcacc aaccccgctt
     1861 ccttcagcgg gttgatcgcc acgatgcgtg tgcccgcgcg cttggccgcc tccaacgtcg
     1921 acagcatccg cgggtggttg gtacccgggt tctggccgac gatcacgacc atgtcggctt
     1981 gggcgaagtc ggtgatgtgc accgacccct tgccgatgcc gatggtctgg gtcagcgcca
     2041 gcccgagcgg caacatgccg gcgatcagga agatcgtg
//
LOCUS       DAORNF010000635         2077 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_39882_length_2077_cov_1.485163, whole genome shotgun sequence.
ACCESSION   DAORNF010000635 DAORNF010000000
VERSION     DAORNF010000635.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39882_length_2077_cov_1.485163"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>312
                     /locus_tag="PLV68_19155"
     CDS             <1..>312
                     /locus_tag="PLV68_19155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03423.1"
                     /translation="TEAVGWQGGGGFRYLKLAPSLLETDKWGREVISKAYNAEMLAEA
                     LCKIEGFTYAPSDAVYWQHGHSTERDFIYVTTQTLGVEQLQQLSEEVGSERSLLVLCS
                     AF"
     assembly_gap    313..393
                     /estimated_length=81
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            495..1466
                     /locus_tag="PLV68_19160"
     CDS             495..1466
                     /locus_tag="PLV68_19160"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRE03424.1"
                     /translation="MGAADEPPCEFHRRPPESSPAAASVSRNPGSDYRDRAADEERTA
                     RKAMLQQKEIDRLQSIVDRFGAKATKAAMAHSKEKQIARLESQKVQSSDNDRVLKVRL
                     PEPPKSGETSVVAESLCKGYGSGAPVFEDVSFDLGRGERLLVLGLNGAGKTSLLRILA
                     GETDATLGTFRFGHNVSVGYYAQEHDNLRSDQSLLDNIRASVPSHLTFTETQLRGMLG
                     MFGLMGDKVFQEAGTLSGGEKTKLALAMLMVGRNNMLLLDEPTNNLDPASRQAVADAL
                     SAWKGTIIFVSHDTEFVEQLDPTKVLLMPDGQVDYFNADWLELVSLA"
     assembly_gap    1618..1705
                     /estimated_length=88
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1706..>2077)
                     /locus_tag="PLV68_19165"
     CDS             complement(<1706..>2077)
                     /locus_tag="PLV68_19165"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRE03425.1"
                     /translation="NIPPGAILIRDGGEAAAWIDWAAANTDILQSVGLGYQGHLGVGQ
                     GFAIGAQLAHPDRRVVQVTGDGAIGFHIQEWDTMVRHDLPIVTVVLNNSCWGMSIHGQ
                     HAVYGDEGDVISRLAPTRYDL"
BASE COUNT          350 a          600 c          636 g          322 t
ORIGIN      
        1 accgaggcag ttggctggca aggcggtggc ggtttccgct atctcaaact ggcgccctcg
       61 ctgctcgaaa ccgataaatg gggccgagag gtcatcagca aggcctacaa cgccgagatg
      121 ctggccgaag ccttgtgcaa gatcgagggc ttcacctatg cgccgagcga cgcggtctat
      181 tggcagcatg gccattccac ggaacgcgat ttcatctatg tgacgaccca gacgctcggc
      241 gtcgagcaac tccagcaatt gagcgaggag gtcggttccg aacgctcgtt gctcgtgctg
      301 tgttccgcct tcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngaacgtc gagaacctgc ctaaggcgcc
      421 tccgaagctg gagcccgagg cgacacccac ggcgaaagcc ggtgcggggc agccggacct
      481 gttcggcgcg aagaatgggg gcagccgatg aaccgccatg tgaattccat cgccggccgc
      541 ctgagtcttc gcccgccgca gcgtcagtct ctcgaaatcc tggatcggat taccgagatc
      601 gcgccgccga cgaagagcgc accgcacgca aggcgatgct gcaacagaaa gagatcgacc
      661 ggttgcagtc gatcgtcgat cgcttcggcg ccaaggccac caaggccgcg atggcgcaca
      721 gcaaggagaa gcagatcgcc cggctggagt cgcagaaggt gcagtcgtcc gacaacgatc
      781 gggtgctcaa ggtgcgcctg cccgagccgc ccaagtcggg tgagacctcg gtggtggccg
      841 agagcttgtg caagggctac ggcagcggtg ccccggtgtt cgaggacgtg tcgttcgacc
      901 tcggccgcgg cgagcggttg ttggtgctgg gtctcaacgg cgccggcaag accagcctgc
      961 tgcgcatcct ggccggtgaa accgatgcga ccctcggcac cttccgcttc gggcacaacg
     1021 tgtctgtggg ctactacgcg caagagcacg acaacctacg ttccgatcag tcgttgctcg
     1081 acaacatccg cgcctcggtg ccatcacatc tcacgttcac cgagacccag ttgcggggca
     1141 tgctcggcat gttcgggctg atgggcgaca aggtgttcca agaggccggc acgctcagcg
     1201 gtggggagaa gaccaagctc gcgttggcga tgctgatggt cgggcgcaac aacatgttgc
     1261 tgctcgacga gccgaccaac aacctcgatc cggccagccg ccaagcggtg gccgacgcgc
     1321 tgtcggcatg gaagggcacg atcatcttcg tcagccacga caccgaattc gtcgaacagc
     1381 tcgacccgac caaggtgctg ttgatgcccg acggccaggt cgactacttc aacgccgact
     1441 ggctcgaact ggtgtcgctg gcctgattca gacgtacagg ctcggcgctg gtgcggtcag
     1501 cgggcgggga tgttgtcgta gtaggggatc acgatctcgc cctccgcgtc gaggtcgccg
     1561 agcatcgagg tggtgatcgg gtggaccacg ctggccgcag tggccacgtt gatcaccnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnccagg tcgtagcggg tgggtgcgag gcgcgagatc
     1741 acgtcgccct cgtcgccgta cacggcgtgc tggccgtgga tcgacatgcc ccagcacgag
     1801 ttgttcaaca ccaccgtcac gatcggcaga tcgtggcgaa ccatcgtgtc ccactcctga
     1861 atgtggaagc cgatcgcacc gtctccggtg acttgcacca cccgccgatc ggggtgggcg
     1921 agttgggcgc cgatcgcgaa gccctgtccc acgccgaggt ggccctgata gccgaggccg
     1981 accgattgga ggatgtcggt gttcgccgcg gcccagtcga tccaggccgc cgcttcgccg
     2041 ccgtcgcgga tcaggatcgc gccgggcggg atgttgg
//
LOCUS       DAORNF010000636         2076 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_39899_length_2076_cov_1.662543, whole genome shotgun sequence.
ACCESSION   DAORNF010000636 DAORNF010000000
VERSION     DAORNF010000636.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39899_length_2076_cov_1.662543"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="PLV68_19170"
     CDS             complement(<1..321)
                     /locus_tag="PLV68_19170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03426.1"
                     /translation="MVAIGGMIGALVSGNVADFFVQANHPSARISVAGISRIASAPFF
                     LVAFSIDVTAVAIISLGVAAMFLVAGIPPTNAARADVLHPNMRGRGASFDAVAQSLAS
                     AASPI"
     assembly_gap    353..382
                     /estimated_length=30
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<383..1193)
                     /locus_tag="PLV68_19175"
     CDS             complement(<383..1193)
                     /locus_tag="PLV68_19175"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRE03427.1"
                     /translation="MTVIDEHSRSILAPSHTPRPNFHLVRKLVGVDKWPLVLLTVIYT
                     INVSDQFLLPAMFPLLKREFGLTDTALGVLSGSYLVSVTVFTVPFGIFADRFTRTRII
                     AWGTCAWGLTMIFTGMSQGFIMLLIGRMVLGMWDPCDNPTSQSLLADYYPVKQRSKVM
                     GIYQMGQLVGFVLLPIAGIMSQAWGWRATFYFFALPAFVVALLAWRLKEPPRGVQDRR
                     HQRLEDHGLAVESAYERRGGIAVYRELLACRSYIALLVSATLGSVFFGGLGV"
     assembly_gap    1305..1314
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1357..>2076
                     /locus_tag="PLV68_19180"
     CDS             1357..>2076
                     /locus_tag="PLV68_19180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03428.1"
                     /translation="MTQPSVCPAQFDHHAPDFAQDPWGVLAELRTSCPVAHSEMYDGF
                     WVLTAYADIKEVATDDVRFSSAQGLTVPDKKNRGQRSIPAEVDPPEFLAYRRILHPML
                     SPTAVDRLTPVIERFVHAAIDNVIEAGRCDFVHDLADPVPAMTTLYKLGLPLDRWKEF
                     SEPLHQVVFMRQDNPARSGAIGQLKVISDTLYETIAARRESPRDDMISYLLHSDIDGR
                     PLKEIVRERGRLMKEAGDASPG"
BASE COUNT          398 a          703 c          632 g          303 t
ORIGIN      
        1 gatcggcgat gccgccgagg ccaagctctg cgcgaccgcg tcgaacgacg ccccgcggcc
       61 gcgcatgttc gggtgcagca cgtcggcgcg agccgcgttg gtcgggggga tgccggcgac
      121 caagaacatc gccgcgacgc cgagcgagat gatggccacg gctgtgacgt cgatgctgaa
      181 cgcgacgagg aagaacggtg ccgacgcgat ccgcgagata ccagcgacgc tgatccgcgc
      241 cgacggatgg ttggcctgga caaagaagtc ggcgacgttg cccgacacca gcgcgccgat
      301 catgccgccg atggcgacca ccgcaatgct cgtggtcgcg gctgcgatcg acnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnagacgccg aggccaccga agaacacgct gccgagcgtg
      421 gccgacacca agagcgcgat gtacgagcgg cacgccagca gttcccggta gaccgcgatg
      481 cccccgcgcc gctcgtaggc cgactcgacc gcgagtccgt ggtcctccag tcgctgatgc
      541 cgccggtcct gcacaccgcg cggtggctcc ttgaggcgcc aggcaagcag cgcgacgaca
      601 aaggccggaa gcgcgaagaa gtagaacgtg gcgcgccagc cccaggcctg gctcatgatg
      661 ccggcgatcg gcaacaggac gaacccgacg agctgaccca tctggtagat gcccatcacc
      721 ttcgaccgtt gcttgaccgg gtaatagtcg gccagcagcg actgcgacgt cgggttgtcg
      781 caggggtccc acatgccgag caccatccgc ccgatcagca gcatgatgaa cccctgcgac
      841 attccggtga agatcatcgt caagccccac gcacaggtgc cccacgcgat gatgcgggtg
      901 cgggtgaacc ggtcggcgaa gatgccgaat ggcaccgtga agaccgtgac gctcaccaag
      961 tacgagccgc tcagcacgcc cagcgccgtg tcggtcaacc cgaactcacg cttgaggagc
     1021 ggaaacattg ccggcaacaa gaactgatcg ctgacgttga tcgtgtagat cacggtcaac
     1081 agcaccagcg gccacttgtc gacaccgacg agcttgcgca cgaggtggaa gttgggccga
     1141 ggggtgtgag acggggcgag gatcgatcgg ctgtgctcgt cgatgaccgt catccttgcc
     1201 cccctgaccc cttcatcccg gtactcatcc ccaaatgagt gtactgaact tgaccctggc
     1261 gacggtaccg cgaccacgac actcctaccg acgtcaccca tatcnnnnnn nnnngacatt
     1321 tcgtatcccc tgtcgacccg aggaggcgtg gcgcccgtga cccaaccatc cgtctgcccg
     1381 gcccagttcg atcatcatgc ccccgatttc gcacaagacc cgtggggagt gctcgccgag
     1441 cttcgcacgt cgtgtcccgt tgctcacagc gaaatgtacg acggcttctg ggtgctgacc
     1501 gcctacgccg acatcaaaga ggtcgcgacc gacgatgtcc gattctcctc ggcgcaaggc
     1561 ctcaccgtgc ccgacaagaa gaatcgcggt cagcgatcga tcccagccga ggtcgatccc
     1621 cccgagttcc tcgcctaccg ccgcatcctg cacccgatgc tctccccgac cgccgtcgac
     1681 cggttgacac cggtgatcga gcgtttcgtc cacgccgcga tcgacaacgt gatcgaggcg
     1741 gggcggtgcg acttcgtcca cgacctcgcc gacccggtcc cggcgatgac caccctctac
     1801 aagctcggcc taccactcga tcggtggaag gagttctccg agccgctgca ccaggtcgtg
     1861 ttcatgcgcc aggacaaccc ggcccggtcc ggcgccatcg ggcaactgaa ggtgatcagc
     1921 gacacgctct acgagaccat cgcggcacga cgggaatcgc ctcgcgacga catgatctcg
     1981 tacctcctcc acagcgacat cgatggtcgc ccgctgaagg agatcgtgcg cgaacggggc
     2041 cggctcatga aagaggccgg ggacgcctcg ccgggc
//
LOCUS       DAORNF010000637         2069 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_40063_length_2069_cov_1.473188, whole genome shotgun sequence.
ACCESSION   DAORNF010000637 DAORNF010000000
VERSION     DAORNF010000637.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40063_length_2069_cov_1.473188"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="PLV68_19185"
     CDS             complement(<1..247)
                     /locus_tag="PLV68_19185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase"
                     /protein_id="HRE03429.1"
                     /translation="MSANEDVKRASNYLRGQIAAELANDEDEFTHDSTLLLKFHGIYQ
                     QDDRDIRRARAAKRLPLDYSCMVRAAIPGGHITPEQWL"
     assembly_gap    270..288
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<289..1611)
                     /locus_tag="PLV68_19190"
     CDS             complement(<289..1611)
                     /locus_tag="PLV68_19190"
                     /inference="COORDINATES: protein motif:HMM:TIGR02034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRE03430.1"
                     /translation="MSTSIDEHVIDQDDQRADLGADPHADPHAGDGVSPRLGTLRIAT
                     AGSVDDGKSTLIGRLLHDTKTVFEDQLSAVVKASRRYGDGETNLALLTDGLRAEREQG
                     ITIDVAHRYFATPQRSFIVADTPGHAQYTRNMVTGASTADVAIVLVDARHGVVEQTRR
                     HAFIAGLLGVDAIVVAVNKMDLVGWDADTFHGIAAAVEQYVAALPAPVPVVAVPLSAL
                     LVVDRSVNTPWYGGPALLELLERFEPTTRIEVGGRVHVQRVIRPQGGVHADFRGYAGV
                     LAGGRLAIGDAITVLPSGRRSSVAGLHRAGRPVEVAFAGQALVVELADDVDVARGDVL
                     VAGASDVEPLVGERVVADVCWMIDTPLRVGNRYWFKHGRRGGRATVVAIEHPHDLHTL
                     EREDADHLELNDLGRVHFDLTEPIVADRYADNPDGGRMILIDELTNTTA"
     gene            complement(1611..>2069)
                     /gene="cysD"
                     /locus_tag="PLV68_19195"
     CDS             complement(1611..>2069)
                     /gene="cysD"
                     /locus_tag="PLV68_19195"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002855962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HRE03431.1"
                     /translation="FRDRQGRWDPRQQRPEPWGMLNARIRKGEHLRAFPLSNWTEVDI
                     WNYIASHDVPLPSIYFSHRRSVVERDGMLLGVGGPVVLEPGEVPFEATVRYRTVGDAS
                     CTGAVESGATTVADVIAEVAAARITERGATRADDNFSESAMEDRKREGYF"
BASE COUNT          321 a          691 c          705 g          333 t
ORIGIN      
        1 ccagccactg ctcgggcgtg atgtgtccac cggggatcgc ggcccgcacc atgcagctgt
       61 agtcgagcgg caaccgcttg gcggcgcggg cgcggcggat gtcgcggtcg tcctgctgat
      121 agatgccgtg gaacttgagc agcagggtgc tgtcgtgggt gaactcgtcc tcgtcgttgg
      181 cgagctctgc cgcgatctgg cccctcaggt agttactcgc gcgtttgaca tcctcgttgg
      241 cactcatggg ctgaccgcgt cgatcatcgn nnnnnnnnnn nnnnnnnngg cggtggtgtt
      301 ggtgagctca tcgatcaaga tcatccggcc accgtcgggg ttgtcggcgt agcgatcggc
      361 gacgatcggt tcggtcaggt cgaagtggac ccggccgaga tcgttcagtt cgagatgatc
      421 ggcatcctcc cgttccaacg tatgcagatc gtgaggatgc tcgatcgcga cgacggttgc
      481 ccgcccgccg cggcggccat gcttgaacca gtatcggttg ccgacccgca gcggggtgtc
      541 gatcatccag cagacgtccg cgaccactcg ctcgccgacc aacggctcga catcggacgc
      601 accagccacc agcacgtcgc cacgggcgac gtcgacatcg tcggccagct caaccaccag
      661 cgcttgtccg gcgaacgcga cctcgaccgg gcgcccagca cggtgcaaac cggccacgct
      721 gctgcggcga ccgctcggca acacggtgat cgcgtcaccg atcgccaggc gcccgccggc
      781 gagcacgccg gcatagccgc ggaagtcggc gtggacaccg ccctgcgggc ggatcacccg
      841 ctgcacgtgg acccgtccgc cgacttcgat gcgggtggtc ggctcgaacc gttcgagcag
      901 ttcgagcagc gccgggccgc cgtaccacgg tgtgttgacc gaccgatcga ccacgagcag
      961 tgccgacagt ggcaccgcca cgacgggcac cggcgcgggc agcgcggcaa cgtactgctc
     1021 gaccgctgcc gcaatgccgt ggaacgtgtc ggcgtcccag ccgaccaggt ccatcttgtt
     1081 gaccgccacc acgattgcgt cgaccccgag caacccagcg atgaacgcgt ggcgacgggt
     1141 ctgctcgacc acgccgtgac gagcgtcgac cagcacgatc gcaacgtcgg cggtcgaggc
     1201 accggtgacc atgttgcgcg tgtactgggc gtggccgggc gtgtcggcga cgatgaagct
     1261 gcgctgtggg gtggcgaagt agcggtgggc gacatcgatg gtgatgccct gctcgcgctc
     1321 ggcccgcaac ccgtcggtca gcaaggccag gttggtctcg ccgtcgccgt agcgacggct
     1381 ggccttgacc acggcagaga gctggtcctc gaacacggtc ttggtgtcgt gcagcaggcg
     1441 gccgatcagg gtgctcttgc cgtcgtcgac cgaaccggcc gtggcgatgc gcaacgtgcc
     1501 caaccggggg gacacgccat caccggcatg ggggtcggca tgggggtcgg cacccaggtc
     1561 ggcgcgctgg tcgtcctgat cgatgacgtg ctcgtcgatg ctggtgctca tcagaagtac
     1621 ccctctcgct tgcggtcctc catggcggac tcgctgaagt tgtcgtcggc acgggtggcg
     1681 ccgcgctcgg tgatacgtgc cgcggcaacc tctgcgatca cgtcggcgac cgtggtggca
     1741 cccgactcga ccgccccggt gcagctcgcg tcgccgaccg tgcggtagcg gaccgtggcc
     1801 tcgaacggga cctcgccggg ttcgagcacc accggcccac cgacgcccag caacatcccg
     1861 tcgcgctcga ccaccgagcg ccgatgactg aagtagatgc tcggcagtgg cacgtcatgg
     1921 ctggcgatgt agttccaaat gtcgacctcg gtccagttcg acaacgggaa ggcgcgcagg
     1981 tgctcaccct tgcggatgcg ggcgttgagc atgccccatg gctcgggacg ctgctgacgg
     2041 ggatcccaac ggccctgccg gtcacggaa
//
LOCUS       DAORNF010000638         2058 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_40325_length_2058_cov_1.770844, whole genome shotgun sequence.
ACCESSION   DAORNF010000638 DAORNF010000000
VERSION     DAORNF010000638.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40325_length_2058_cov_1.770844"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            448..>901
                     /locus_tag="PLV68_19200"
     CDS             448..>901
                     /locus_tag="PLV68_19200"
                     /inference="COORDINATES: protein motif:HMM:TIGR04551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03432.1"
                     /translation="MAAAQIGPGLPTSPGGPGAQQEEKKDGVAEAAPKATGALPTTPV
                     LPSPRSKRKRWKLLEVDGYYRLRTDWFKNFNLGFNDDPALGGAPFPQALSCNTDPQVS
                     ACDDTMSGANMRLRLEPTINIDEGTGVHLQLDVYDNLVLGSTPAGESAG"
     assembly_gap    902..930
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <931..>2058
                     /locus_tag="PLV68_19205"
     CDS             <931..>2058
                     /locus_tag="PLV68_19205"
                     /inference="COORDINATES: protein motif:HMM:TIGR04551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04551 family protein"
                     /protein_id="HRE03433.1"
                     /translation="PLGAFGDGQGPVVRGQNSDRDPIVVKRAWAEVALPLGILKFGRM
                     PNHWGMGIMNNAGGEDPINGTYNYDGDYGDTVDRVSFSAIIPGTQLRGMVASDWSLTR
                     LTSNQVAAGAGRDGHPFDLDDADDANQWVFTISKMDTPTELRDQLDRNELVVNYGLYL
                     AYKTQSWDIDLRDFTPGGTLGATRFVPRDLTSYTPDVWLRVGYGAHQFELEAALEFGS
                     ISRLDDQGLTGSVDIAKFGGVGRYTWRGLDDKLVLGAEIGMATGDQWDNTPQGSTHIS
                     NANLIGGAGDSTLSQFIFDREYQVDMILFRHLMGAVTNATYVKPFLSYQLTDSIYFRG
                     SNINSFAMRKVATPGNDLMYGIELNGEAGYRSGGFQAGLAYG"
BASE COUNT          362 a          751 c          593 g          323 t
ORIGIN      
        1 ctaccggcgg taccgccggt ccacaagcgc cgatccccac acagcaggca acacaaatcc
       61 agggccaacg gtccaagggc atcgcatgca agctagcgca cccgcggggc aaaccgccag
      121 caaacggcct ctccccccgt cgccgccgat ggctcgacga agccccccag ccacgccgcc
      181 gccaggtggg catggttgca caccttcggc cgatctaccg gcgagcggcc aaaccttcct
      241 caccgaattg accagccgta cccccctgtc tatagtccgc gacctctgcg atggcacatc
      301 cttcggtttc ctctgttgtt ttcggcccgc ttcgactccg ggcgctggca caacagtccg
      361 aggcgccatc ggtcttggga gactctcatg agcgcaacta gttttcgtcg actttcactg
      421 ggccttgccc tcgtgctcgt tccatccgtg gccgccgctc agatcggccc cggcttgccc
      481 acctctcctg gcggtccggg cgcccagcaa gaagaaaaga aagacggagt cgcagaagca
      541 gcccccaagg ccacgggcgc gcttcccacc acgccggtgc tgccgtcgcc gcgcagcaag
      601 cgcaagcgct ggaagctcct cgaggtcgat ggctactacc ggctgcgcac cgactggttc
      661 aagaacttca acctcggctt caacgacgat ccagcgctcg gcggcgcgcc gtttccgcaa
      721 gcgctgtcgt gcaacacgga tccgcaagtc agcgcctgcg acgacaccat gtccggcgcc
      781 aacatgcgcc tgcggctcga gcccacgatc aacatcgacg aaggcaccgg ggtccacctg
      841 cagctcgacg tgtacgacaa cctcgtgctg ggctcgaccc cggcaggtga atcggccggc
      901 annnnnnnnn nnnnnnnnnn nnnnnnnnnn ccgctcggcg ccttcggcga tggccagggc
      961 ccggtggtgc gcggccagaa cagcgaccgc gacccgatcg tcgtcaagcg cgcctgggcc
     1021 gaggttgccc tgccgctggg cattctcaag ttcggccgca tgcccaacca ctggggcatg
     1081 ggcatcatga acaacgccgg tggcgaagac ccgatcaacg gcacctacaa ctacgacggc
     1141 gactacggcg acaccgtgga tcgagtgtcg ttctccgcga tcatccccgg cacccagctc
     1201 cgcggcatgg tcgcctccga ctggtcgttg acccgcctca cctccaacca ggtggcggcc
     1261 ggcgctggcc gcgacgggca cccgttcgac ctcgacgacg ccgatgatgc caaccagtgg
     1321 gtgttcacca tctccaagat ggacacgccg accgagctgc gcgaccagct cgatcgcaac
     1381 gagctggtgg tcaactacgg tctctacctg gcctacaaga cgcaatcttg ggacatcgac
     1441 ctgcgcgact tcacgccggg cggcacgctc ggcgccaccc gcttcgtgcc acgtgatctg
     1501 acctcttaca cgcccgatgt ctggctgcgc gtcggctacg gcgctcacca gttcgagctg
     1561 gaggcggcgc tcgagttcgg ctccatctcc cgcctcgacg accaggggct caccggcagc
     1621 gtcgatatcg ccaagttcgg cggcgtcggc cgctacacct ggcgcggcct cgacgacaag
     1681 ctggtgctcg gcgccgagat cggcatggcc accggtgacc agtgggacaa caccccgcaa
     1741 ggctcgaccc acatcagcaa cgccaacctc atcggcggcg ctggcgactc cacgctctcg
     1801 cagttcatct tcgatcgcga gtaccaggtg gacatgatcc tgttccgcca cctgatgggc
     1861 gccgtcacca acgccacgta cgtcaagccg ttcctgagct accagctcac cgactcgatc
     1921 tacttccgcg gctcgaacat caactccttc gcgatgcgca aggtggcgac cccgggcaac
     1981 gatctgatgt acggcatcga gctcaatggc gaggccggct atcgctcggg cggcttccag
     2041 gcggggctcg cgtatggc
//
LOCUS       DAORNF010000639         2057 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_40352_length_2057_cov_2.029471, whole genome shotgun sequence.
ACCESSION   DAORNF010000639 DAORNF010000000
VERSION     DAORNF010000639.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40352_length_2057_cov_2.029471"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1356)
                     /locus_tag="PLV68_19210"
     CDS             complement(<1..1356)
                     /locus_tag="PLV68_19210"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF015448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HRE03434.1"
                     /translation="MKIVLSWLNDLAPIGDDVEAIATDLTQLGIQVEAIEQVGSTVDG
                     VITARVLRTERHPDAAKVHRVYVTTSDGVGEDADERHVWCGAFNMQPGDVIPLATPGT
                     VMPDGRAIEPRPILGIDSQGMLCSARELGLGDDHSGILILPADTPLGVPYGEALGLTT
                     ETVFDADLTRNRPDCWGHRGVARDLAAHRGVALREVAMAPQADGPSRSAPVELIDGDR
                     CTRFTTLVLSGIAVAPSPDWVARRLTAAGMRPINNVVDASNLVMLELNQPNHAYDLDA
                     LGGGGFRIRLARDGETITTLDDAERALTTDDLLICDAHDVPIGVGGIIGGRDSEITDA
                     TTVVALEIAHFEQTAITRSMNRLGLRTEAGGRFERGVDPYGMPMAQARFVEVLAETCP
                     DLVVHDGAVDARSPALMPQVRPVQVRVSQVNRILGTSLDAAAVAALITPIGFATHIRA
                     DA"
     gene            complement(1367..>2057)
                     /gene="pheS"
                     /locus_tag="PLV68_19215"
     CDS             complement(1367..>2057)
                     /gene="pheS"
                     /locus_tag="PLV68_19215"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HRE03435.1"
                     /translation="RAHPHLSTQAWERLEDVFIGLGFQIAEGPEVETDFYNFEALNMP
                     PSHPARSGFDTMFTDYRDDGSVLLRTHTSPVQIRVMQTIAPPIYMVMPGKVFRRDTPD
                     ATHMPVFHQIEGLVVDRGITMAHLAGTIDAFTKAFFGPGFNSRLRPNYFPFTEPSAEF
                     DIQRPDGGWIELGGCGMVHPNVLRAGGLDPEEWSGFAFGFGIDRMARERHGVADLRDM
                     FSNDVRFIEQF"
BASE COUNT          319 a          672 c          742 g          324 t
ORIGIN      
        1 ggcatcggca cggatgtgcg tggcgaaccc gatcggggtg atcagtgccg cgaccgccgc
       61 ggcatcgagg ctggtaccca ggatacggtt gacctggctg acccgcacct gcaccgggcg
      121 cacctgcggc atcagcgccg gggagcgagc atcgacagca ccgtcgtgca cgacgaggtc
      181 ggggcaggtc tcggccagca cctcgacgaa gcgggcctga gccatcggca tgccgtacgg
      241 atcgacgccg cgttcgaagc gaccgccggc ctcggtgcgc aggccgaggc ggttcatcga
      301 gcgggtgatc gcagtctgtt cgaagtgagc gatctccagc gcgaccacgg tggtggcgtc
      361 ggtgatctcg ctgtcgcggc cgccgatgat gccgccgacg ccgatcggta cgtcgtgcgc
      421 gtcgcagatc agcaagtcat cggtggtgag ggcgcgctcg gcatcgtcga gcgtggtgat
      481 cgtctcgccg tcgcgggcca agcggatgcg gaagccaccc ccgcccaggg cgtcgaggtc
      541 gtaggcgtgg ttgggctggt tgagttccaa catcaccagg ttgctggcgt cgaccacgtt
      601 gttgatcggg cgcatccccg ccgccgtcag ccgccgggcc acccagtcgg gcgacggcgc
      661 gaccgcaatc ccggacagca ccagggtggt gaagcgggtg caccggtcgc cgtcgatcag
      721 ttcgacgggc gcgctgcgcg acggaccatc ggcctgggga gccatcgcca cctcgcgcag
      781 cgccacgccg cggtgcgcgg ccagatcacg ggcgacaccg cgatggcccc agcagtcggg
      841 ccggttgcgg gtgaggtcgg cgtcgaacac cgtctcggtg gtgagcccca acgcctcgcc
      901 gtagggcaca cccaacgggg tgtcggctgg caggatcagg atgcccgagt ggtcgtcgcc
      961 gaggccgagt tcgcgcgccg agcagagcat gccctggctg tcgatgccca ggatcgggcg
     1021 cggctcgatg gcccgcccgt cgggcatcac cgtgcccggg gtggcgagcg ggatcacgtc
     1081 gcccggctgc atgttgaacg cgccgcacca cacgtggcgc tcgtcggcgt cctcgcctac
     1141 accgtcactc gtagtgacgt agacgcggtg caccttggcc gcgtcgggat gacgctcggt
     1201 gcgtagcacc cgggccgtga tcacaccgtc gacggtgctg ccgacctgtt cgatcgcttc
     1261 gacttggatg ccgagctggg tgaggtcggt ggcgatggct tcgacgtcgt cgccgatcgg
     1321 cgccaagtcg ttcagccagg agagcacgat cttcatctgg acctcctcag aactgctcga
     1381 tgaagcggac gtcgttgctg aacatgtcgc gcagatcggc cacgccgtgg cgctcgcgag
     1441 ccatgcggtc gatgccgaag ccgaatgcga acccgctcca ttcctcagga tccaaccccc
     1501 cagcgcgcaa cacgttgggg tgcaccatgc cgcagccgcc gagctcgatc cagccgccgt
     1561 cggggcgctg gatgtcgaac tccgccgacg gctccgtgaa cgggaagtag ttgggccgca
     1621 gccggctgtt gaagcccggc ccgaagaacg ccttggtgaa ggcgtcgatc gtgccggcca
     1681 gatgggccat ggtgatgccg cgatcaacga ccagaccctc gatctggtgg aaaaccggca
     1741 tgtgcgtggc gtcgggggtg tcgcggcgga acaccttgcc cggcatgacc atgtagatcg
     1801 gcggggcgat ggtctgcatc accctgatct gcaccggcga ggtgtgggtg cgcagcagca
     1861 ccgacccgtc gtcgcggtag tcggtgaaca tcgtgtcgaa gccgctgcgc gctgggtgac
     1921 tgggcggcat gttcaacgcc tcgaagttgt agaagtcggt ctcgacctcg gggccttctg
     1981 cgatctggaa gccgagaccg atgaacacgt cctccagccg ctcccaggcc tgggtcgaca
     2041 ggtgggggtg ggcgcgg
//
LOCUS       DAORNF010000640         2051 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_40502_length_2051_cov_1.487475, whole genome shotgun sequence.
ACCESSION   DAORNF010000640 DAORNF010000000
VERSION     DAORNF010000640.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40502_length_2051_cov_1.487475"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="PLV68_19220"
     CDS             complement(<1..272)
                     /locus_tag="PLV68_19220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03436.1"
                     /translation="MRVAHGEGGGAGWVHDSALRSLEWGWAGENESALCGEPEKATKG
                     VGMLDVVIRGGTIVDGTGAAARSGDVGIRDGRIVTVGERITEPTRD"
     assembly_gap    371..384
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(626..>1075)
                     /locus_tag="PLV68_19225"
     CDS             complement(626..>1075)
                     /locus_tag="PLV68_19225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRE03437.1"
                     /translation="AMATFEATRPSVGAQALGIARAAYEYSLEYAKERRAFGRPIIMN
                     QAIAFKLANMALEIDASRLLIWRAAWLARNGGFKNGEGSMSKYKASEVAVRVTEDAIQ
                     ILGGYGYTREYPVERWHRDAKIHTIFEGTSEIQQLVIARAISGLRIE"
     assembly_gap    1076..1131
                     /estimated_length=56
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1132..1852)
                     /locus_tag="PLV68_19230"
     CDS             complement(<1132..1852)
                     /locus_tag="PLV68_19230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012226402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRE03438.1"
                     /translation="MADFSLLLNDDQNQIKDWVHTFAEDVIRPVAHEWDEREEFPWPV
                     VQEAAQIGLYGFEFMLNAMSDPTGLTLPVAIEELFWGDAGIGMAIMGSALAAAGISGN
                     GTPEQVMEWVPQCYGTPDDVKLGAFCVSEPDAGSDVSSLRTRAVYDEANDEWVLNGTK
                     AWITNGGIANVHVVVAAVDPELKGRGQASFIIPPDTKGLSMGQKYLKHGIRASHTSEV
                     VLDDVRIPGRMLLGGKEKLDAK"
BASE COUNT          346 a          672 c          627 g          336 t
ORIGIN      
        1 tcgtcgcgcg tcggctcggt gatccgctcg cccaccgtga cgatgcggcc gtcacgaata
       61 cccacgtcac cgctgcgggc ggccgcgccc gtgccatcga cgatcgttcc cccacgaatc
      121 accacgtcca acatgccgac acctttcgtc gccttctctg gctcgccaca cagcgcagac
      181 tcattctccc ccgcccaccc ccactccaac gagcgcagcg cgctgtcatg aacccagcct
      241 gccccgccgc cctccccatg ggcaacgcgc accaagcgac cgacgcggaa gctcgacaac
      301 gaacagatgc accgaatcag cgcggaagcg ctgtggagca ggtcgtccgc caatcggctt
      361 gccgtgggca nnnnnnnnnn nnnncatact ggtgactgcc cacggcaagc gctgcgggaa
      421 gtggacccgg ttgcctaccg gatggtaggg gaaccgggtt ctgcgcgtta cgggcgcagt
      481 tcacacactt cgtgcacaac tgaatcgacg agcgttctcc aggtgttcgc ggcaccaacc
      541 gcccaggtga gggcatcggc gacccagagc acacgacact gccggatgga tgtggggcga
      601 tgtcggaatg ccaccagcgc gaggactact cgatgcggag gccgctgatg gctcgggcaa
      661 tgacgagttg ttggatttcg ctggtgcctt cgaagatggt gtggatcttg gcgtcgcggt
      721 gccagcgctc gacggggtat tcgcgggtgt atccgtagcc gccgaggatc tggatcgcgt
      781 cctcggtgac gcgcacggcg acctcgctgg ccttgtactt gctcatcgag ccttcaccgt
      841 tcttgaagcc gccgttgcgg gccagccacg cggcacgcca gatcagcagg cgcgaggcgt
      901 cgatctccaa cgccatgttg gccagcttga acgcgatcgc ctggttcatg atgatcggcc
      961 gaccgaaggc gcggcgctcc ttggcgtatt cgagcgagta ctcgtaggcg gcccgagcga
     1021 tgccgagggc ctgcgcgccg accgagggac gggtggcctc gaacgtcgcc atcgcnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nacttggcgt
     1141 cgagcttctc cttgccgccc agcagcatcc ggccgggaat gcgcacgtcg tcgagcacga
     1201 cctcggacgt gtgcgaggca cggatgccgt gcttgagata cttctgcccc atgctgaggc
     1261 ccttggtgtc cggcgggatg atgaagctgg cctgaccacg gcccttcagt tcggggtcga
     1321 ccgcagcgac cacgacgtgg acgttggcga tgccaccgtt ggtgatccac gccttggtgc
     1381 cgttgagcac ccactcatcg ttggcctcgt cgtacaccgc acgggtgcgc agtgacgaga
     1441 cgtcggagcc ggcgtccggc tcactgacgc agaaggcgcc gagcttgacg tcgtcgggcg
     1501 tgccgtagca ctgcggcacc cactccatca cctgctcggg cgtgccgttg ccggagatgc
     1561 ccgcggcggc gagggccgag cccatgatcg ccatgccgat gccggcatca ccccagaaca
     1621 gctcttcgat cgcgaccggc agggtgagcc ccgtcgggtc gctcatcgcg ttgagcatga
     1681 actcgaaccc gtacaggccg atctgggccg cctcctgcac gacgggccag gggaactcct
     1741 cccgctcgtc ccattcgtgg gcaaccggac ggatgacatc ttcggcgaag gtgtggaccc
     1801 aatccttgat ctggttctga tcgtcgttga gcaagagcga gaagtctgcc atgggcacta
     1861 agttaccagt cggtaaccag accatcaacc tcgctcagcc aggggatcag gtcggtcgtg
     1921 tggcaccatg accatcgcac tcggcgggga ggtcgggcgc gccaatcgaa cgagagatca
     1981 ccatgcatcc aacgccgcag cacggcagca ccactcatca tcctcaccac aatccggcgc
     2041 actcgacgtg g
//
LOCUS       DAORNF010000641         2050 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_40523_length_2050_cov_1.791479, whole genome shotgun sequence.
ACCESSION   DAORNF010000641 DAORNF010000000
VERSION     DAORNF010000641.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40523_length_2050_cov_1.791479"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            295..>584
                     /locus_tag="PLV68_19235"
     CDS             295..>584
                     /locus_tag="PLV68_19235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRE03439.1"
                     /translation="MGNRDNFTGVIGRTFADSTPAWPAPDTAPAGAPNVVLVLLDDVG
                     YAQFGCYGSDIATPTFDRLAAGGLRYANYHTTALCSPTRACLLTGRNHHASG"
     assembly_gap    585..774
                     /estimated_length=190
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <775..>1055
                     /locus_tag="PLV68_19240"
     CDS             <775..>1055
                     /locus_tag="PLV68_19240"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRE03440.1"
                     /translation="RYYGFLAGETDQYNPDLIADNHQVPPPRTAAEGYHLTEDLIDHA
                     IDYVKDLRAYSPTKPFFLYVAPGACHAPHQAPRDYIDAYRGRFDMGWDQ"
     assembly_gap    1056..1149
                     /estimated_length=94
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1150..>1418
                     /locus_tag="PLV68_19245"
     CDS             <1150..>1418
                     /locus_tag="PLV68_19245"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRE03441.1"
                     /translation="DDERRLYARMMEVYAGFMTHTDAQIGRLVDFLDAHGELDNTIFV
                     VMSDNGASAEGGPQGSFNEQYFFNFVPERLEENLRRIDDLGGPHA"
     assembly_gap    1419..1428
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1429..>1766
                     /locus_tag="PLV68_19250"
     CDS             <1429..>1766
                     /locus_tag="PLV68_19250"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRE03442.1"
                     /translation="AWAGNTPLKRFRRDTHEGGVTDPFIVHWPKGLGDLGRGDEGGAG
                     RTRHQYVHAIDVAPTLLDLIGIEPPSHINGIAQSPHDGVSVAATLRDPTAPDRHVTQY
                     YEMLGSRAIY"
     assembly_gap    1767..1792
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1793..>2050
                     /locus_tag="PLV68_19255"
     CDS             <1793..>2050
                     /locus_tag="PLV68_19255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03443.1"
                     /translation="PAMMVYDGTDAGTRSFDDDTWELYHVAEDFSECHDVAAAHPDKL
                     AELQELWWREAERFQVLPLNNQPGKFGDNRFRRERYVYMPG"
BASE COUNT          319 a          627 c          548 g          236 t
ORIGIN      
        1 aacacgacac tggcggccgc ggcagcgaca gcgaggggcg cagcgacagc ggctgcgggg
       61 cgacgggtgg agatgaggcg tggcacgctc gcctgagtgc ctgcagtgcc ccaacccgga
      121 aatcgcgcct cgccgacggt tgccacggcg accggtcgag atcacggcaa acacgactgg
      181 tacggtccgc cgccagggcc actcggggcg tgacataatc tgtcagtcca accgacagaa
      241 catcgtcggg cgcaccgctg ccctcgtcgg gggacgcaca gagaggggat gcacatgggg
      301 aaccgcgaca acttcacggg cgtgatcggg cgaaccttcg ccgactccac accggcgtgg
      361 cccgcgcccg acacggcgcc cgcgggggca cccaacgtgg tgttggtgct gctcgacgat
      421 gtgggctacg cccagttcgg ttgctatggc agcgacatcg cgacgcccac cttcgatcgc
      481 ctcgccgcgg gcggtctgcg ctacgccaac taccacacga ccgcgctgtg ctcgccgaca
      541 cgggcgtgcc tgctgaccgg acgcaaccac cacgccagcg gcatnnnnnn nnnnnnnnnn
      601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncgctac
      781 tacggcttcc ttgccggcga gaccgaccag tacaaccccg acctcatcgc cgacaaccac
      841 caggtgccac cgccgcgcac cgcggcagag ggttaccacc tcaccgagga tctgatcgac
      901 cacgcgatcg actacgtcaa ggatctgcgc gcctactccc ccaccaagcc gttctttctc
      961 tacgtcgcac ccggcgcctg ccacgccccg caccaagcgc cgcgcgacta catcgacgcc
     1021 taccgcggca ggttcgacat gggttgggac cagtgnnnnn nnnnnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnng acgacgaacg ccgtctctac gcgcggatga tggaggtcta cgccgggttc
     1201 atgacccaca ccgacgccca gatcggccgc ctggtcgact tccttgacgc ccacggcgag
     1261 ttggacaaca cgatcttcgt ggtgatgagc gacaacggcg cgtcggccga aggcggcccg
     1321 caggggtcgt tcaacgagca gtacttcttc aacttcgtac ccgagcggtt ggaggagaac
     1381 ctgcgccgca tcgacgacct cggcggtccg cacgccaann nnnnnnnngg cgtgggccgg
     1441 caacacgcca ctcaagcggt tcaggcgcga cacccacgag ggcggggtga ccgacccgtt
     1501 catcgtccac tggcccaagg gcctgggcga cctcggccga ggtgatgagg gtggagccgg
     1561 ccgcacccga caccagtacg tgcatgcgat cgacgtcgcg cccacgctgc tcgacctgat
     1621 cggcatcgaa ccacccagcc atatcaacgg catcgcgcag agcccacacg acggggtgtc
     1681 ggtcgcagcc actttgcgcg atccaaccgc ccccgatcgc cacgtcaccc agtactacga
     1741 gatgctcggc tcgcgggcca tctaccnnnn nnnnnnnnnn nnnnnnnnnn nnaccggcga
     1801 tgatggtcta cgacggcacc gacgccggga cgcggtcgtt cgacgacgac acctgggagc
     1861 tgtaccacgt ggccgaggac ttctccgagt gccacgacgt cgccgccgcg caccccgaca
     1921 agctggccga actgcaggag ctgtggtggc gcgaggccga gcggttccag gtgctgccgc
     1981 tcaacaacca gcccggcaag ttcggcgaca accggttccg ccgcgagcgc tacgtgtaca
     2041 tgccgggcat
//
LOCUS       DAORNF010000642         2043 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_40708_length_2043_cov_1.931087, whole genome shotgun sequence.
ACCESSION   DAORNF010000642 DAORNF010000000
VERSION     DAORNF010000642.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40708_length_2043_cov_1.931087"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            112..900
                     /locus_tag="PLV68_19260"
     CDS             112..900
                     /locus_tag="PLV68_19260"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRE03444.1"
                     /translation="MSELVVEGLTSGYDDAVVIEGVSFTVPERGVTALVGPNGAGKTT
                     LLKTLMGLVPVARGTITFDGHDITATRPRQRARLGIALVAEGRGLFPDLTVGDNLRLG
                     ASAVGHRPDGSLDRVLELFPELHRLAPQRAGSLSGGEQQMCAIGRSLMAQPALLVIDE
                     LSLGLAPVVVDRLADAVRVLARDSGRPLAIVLVEQNVDLALDLADRALGIFDGRLFFD
                     GSAAVARDQIGRFSDAVLGIAPSEPSTSATNLTIPLDFDLQETR"
     gene            897..>1663
                     /locus_tag="PLV68_19265"
     CDS             897..>1663
                     /locus_tag="PLV68_19265"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRE03445.1"
                     /translation="MTSWLHITRGSVIDGSGAPPRPDTDVLVRDDQIVAIGTSLATEQ
                     FVPRGEPLVVLDAFGKTVMPGLIDTHCHVTFGESLTQEEQDFYTGAEIATLRAANNLT
                     RILRSGVTSIAQPGGSYNIGVALRQGRSEGIVAGPRIATSGRFIAVPNALTDFYPTWV
                     GQPPSAQSITVNTVDELRGEIRAQVKNGADFIKLCDSAGGQLQAFTAEELKAGVEIAH
                     QLHRPVTIHARGAAEVKASVRAGVDWIMHGNILDHEA"
     assembly_gap    1664..1723
                     /estimated_length=60
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1724..>2043
                     /locus_tag="PLV68_19270"
     CDS             <1724..>2043
                     /locus_tag="PLV68_19270"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRE03446.1"
                     /translation="GDLAGVPRIRRDRDARLLEYSAVWLERAHKAGVPFALGTDSGFA
                     VTPFGEFHARELEDLQRYAGMTALEAITAGTRNGARMMGHDGELGEVRVGALADLLVV
                     DGDPA"
BASE COUNT          304 a          693 c          667 g          319 t
ORIGIN      
        1 tggccgaccg tgtctgcgtg ctcgatcgcg gccgaccgat cgcgctcggt acacctgccg
       61 acgtgcttgc cgacgcctcg gtgatcaacg cctacctcgg caggagcgcg catgtctgag
      121 ctcgtcgtcg agggtctgac cagtggttac gacgacgctg tcgtgatcga gggcgtgtcc
      181 ttcaccgtgc ccgagcgagg cgtcacggct ctggtcggcc cgaacggggc gggcaagacg
      241 acgctgctca agacactgat ggggctggtg cccgtcgccc ggggcaccat caccttcgac
      301 ggtcacgaca tcaccgcaac ccggccccgc caacgagccc gactcggcat cgccttggtt
      361 gccgaagggc gggggttgtt ccccgatctc accgtcggcg acaacctgcg cctcggtgcg
      421 agcgccgtgg gccaccgacc cgacggctcg ctcgatcgcg tgctcgagtt gttccccgaa
      481 ctgcaccgat tggcacctca gcgcgccggc agcctgtctg gcggcgagca gcagatgtgt
      541 gccatcggac gcagcttgat ggcccagccg gcgctgctcg tgatcgacga gctgtcgctc
      601 ggcttggcgc cggtggtcgt cgaccggttg gccgacgccg tgcgtgtgct cgcccgcgac
      661 tcggggcggc cgctggcgat cgtgctggtc gaacagaatg tcgacctcgc gctcgacctg
      721 gccgatcgcg ccctcggcat cttcgacggg cggctcttct tcgacggttc ggcggcggtc
      781 gctcgcgacc agatcgggcg cttcagcgat gcggtgctcg gtatcgcccc gagcgagcca
      841 tcgacctccg cgaccaacct cactatccct ctcgacttcg acctacagga gacccgatga
      901 cttcgtggct gcacataacc cgtggatccg tcatcgacgg aagcggcgcc ccgccgcggc
      961 ccgacaccga cgtgttggtc cgcgacgatc agatcgttgc gatcggaacg agcctcgcga
     1021 ccgagcagtt cgttcctcgc ggcgagccgc tggtggtgct cgacgcattc ggcaagaccg
     1081 tgatgcctgg gctgatcgac acgcactgcc atgtcacctt cggtgagtcg ctcacccagg
     1141 aggagcagga cttctacaca ggagccgaga tcgccacgct gcgggcggcg aacaacctga
     1201 cccgcatcct gcgctccggc gtcacctcca ttgcccaacc gggtggcagc tacaacatcg
     1261 gtgtcgccct ccgccagggc cgcagcgaag gcatcgtcgc cgggccccgc atcgccacct
     1321 cgggtcggtt catcgcggtg cccaacgcgc tcacggactt ctacccgacc tgggtcggcc
     1381 agcccccgtc ggcccagtcg atcacggtca acaccgtcga cgaactgcgt ggtgagatca
     1441 gggcccaggt gaagaacgga gccgacttca tcaagttgtg cgacagcgcg ggcgggcagt
     1501 tgcaggcctt caccgccgag gagctcaagg caggcgtcga gatcgctcac caactccacc
     1561 ggccggtcac catccacgcc cgcggggcgg ccgaggtgaa ggcctcggtg cgcgccggag
     1621 tcgactggat catgcacggc aacatcctcg accacgaagc gatnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnggcgatc tggccggggt
     1741 accccgcatc aggcgcgacc gcgacgcgcg gctgttggaa tacagcgcgg tgtggctgga
     1801 gcgggcccac aaggcgggtg tgccgttcgc gttgggcacc gacagcggct ttgcggtcac
     1861 ccccttcggt gagttccacg cgcgcgagtt ggaggacctg cagcgttacg ccggcatgac
     1921 cgccttggag gcgatcaccg ccggcacccg caacggggcg cggatgatgg gccacgacgg
     1981 ggaactcggc gaggtgcggg tgggcgcgct ggccgatctg ctcgtcgtcg acggtgatcc
     2041 cgc
//
LOCUS       DAORNF010000643         2041 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_40760_length_2041_cov_1.674723, whole genome shotgun sequence.
ACCESSION   DAORNF010000643 DAORNF010000000
VERSION     DAORNF010000643.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40760_length_2041_cov_1.674723"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="PLV68_19275"
     CDS             <1..237
                     /locus_tag="PLV68_19275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03447.1"
                     /translation="LLVVGSSLAVFSGYRFCKQALACGQPIAALNLGRTRADGELALK
                     VDRPCGPTLRALLDRLQLTPMLAPTFPDPVPAGP"
     gene            330..>2041
                     /locus_tag="PLV68_19280"
     CDS             330..>2041
                     /locus_tag="PLV68_19280"
                     /inference="COORDINATES: protein motif:HMM:NF037016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HRE03448.1"
                     /translation="MGVDREAPDVVIQTPPSGAVVGSLTVVSGMASDDAAGVALIEAR
                     LTNDAGLNWNGSVWTSDAHWLTAGGTSQWSIDGSLPRGDDAQTGAYSLSIRVTDHAGR
                     STLAGPNPLTIWPEAGVHVYELPPPAGAVRHTSATAINTRGTVVGLTGSGQSDPATRW
                     DRGQATLMQLPEGATSSLVSDVNDAGVAVGAVWFDNVSRAASWAADGSVTMLPSLANA
                     PHQSASVSSINNSGVMVGTSGDRPVRWDEGVVTALANPTPNAYGVATAIADSGMIVGE
                     SATANGHRAVIWRNDQPTVLGTLGGAWSRATGVNERGQVIGVSRDRSGRESAFVYDGE
                     TMTAISDGLGAETAEPHAINEAGTVVGEFTRASGSTHAFIWSPGTTARDLGTMAGRDS
                     GWELVRARDVNDYGQIVGFGELNGVGRAFLLSSVHAPMAADVTATTAPHVPVAIDLPL
                     WDPDPLDVIEAEVVSGPANGMITGLDGTVATYRPRDGWVGTDTFTFRAFDGRFGSIVA
                     TATITVAGELPDIPPTTTTTTVPPTTTMPTTTTMPPTTVPTTTVPPTTPSTTTTIPTT
                     TTTVP"
BASE COUNT          328 a          745 c          670 g          298 t
ORIGIN      
        1 ctgctggtgg tgggctcgtc gctcgcggtg ttttccggct atcgcttctg caagcaggcc
       61 ctcgcctgcg gccagcccat cgcggcgctc aatctcggcc gcacccgggc cgacggcgag
      121 ctggcgctga aggtggatcg gccctgcggc ccgacgctcc gcgccctgct ggaccggttg
      181 cagctcactc cgatgctcgc tccgaccttt cccgacccgg ttccagccgg cccatgagct
      241 gaatctcgcc attcgccagc ggccggacca cgatcacctt cgcggccacc gctgccgacg
      301 ggcgaaccga accgttgcgc tcgatcaccg tgggcgtcga ccgtgaagca ccggacgtcg
      361 tcattcagac gccaccctcc ggcgcggtcg tcggttcgct gaccgtcgtg tccggcatgg
      421 cgagcgacga tgcggcaggt gtggcgctca tcgaggcccg cctcaccaac gatgcgggcc
      481 tgaactggaa cggctcggtc tggacgtcgg acgctcactg gttgacggct ggcggaacga
      541 gccagtggtc gatcgacgga tcgctgcctc ggggcgacga cgcccagacc ggtgcctact
      601 cgctcagcat tcgtgtcacc gaccacgccg ggcgttcgac actcgccggc ccgaacccgc
      661 tcaccatctg gcccgaggca ggtgtgcacg tctacgagtt gccgccgcct gccggtgcgg
      721 tgcgccacac cagtgccacg gcgatcaaca cccgagggac ggtggtcgga ctgactggca
      781 gcggccagag tgacccagcc acacgttggg atcgcggcca ggcgaccctg atgcagttgc
      841 cggagggagc cacgtcgtcg ctggtctccg acgtcaacga cgctggtgtc gcggtgggcg
      901 cggtctggtt cgacaacgtc agccgcgccg cgtcatgggc agcggacgga tcggtgacga
      961 tgctgccctc gctggccaat gccccacacc aatcggcgtc tgtgtcgtcg atcaacaaca
     1021 gcggtgtcat ggtcggcacc tccggcgatc gcccagtgcg ctgggacgag ggggtggtca
     1081 ccgccctcgc aaacccgaca ccgaacgcgt acggcgtcgc cacagcgatc gctgacagcg
     1141 gcatgatcgt gggcgagtca gccactgcca acggtcatcg tgccgtgatc tggcgcaacg
     1201 accagccgac ggtgctcggc accctgggcg gcgcgtggag ccgggcgacg ggtgtcaacg
     1261 agcggggcca ggtgatcggc gtgtcgcgcg accgatccgg ccgggagagt gcgttcgtgt
     1321 acgacggcga gacgatgacc gcgatcagcg acggcctcgg tgccgagaca gcggagccgc
     1381 atgcgatcaa cgaggcaggc acggtcgtgg gtgagttcac gcgcgccagc ggctcaaccc
     1441 acgcgttcat ctggagcccc ggcaccacgg cgcgtgacct cggcacgatg gccggacgcg
     1501 acagcggctg ggaactggtc cgggcgcggg acgtcaacga ctacggccag atcgtcggct
     1561 tcggcgaact caacggcgtc ggccgggcct tcctgctgag ctcggtacat gccccgatgg
     1621 cggctgacgt gacagccacg acagcaccgc acgtcccggt ggcgatcgac ctgccgctgt
     1681 gggaccccga cccgctcgat gtgatcgagg ccgaggtcgt ctccggtccg gccaacggca
     1741 tgatcaccgg tctcgacggc acggtcgcga cctaccgtcc tcgcgacggc tgggtcggca
     1801 ccgacacctt cacgttccga gccttcgacg gtcgtttcgg ctcgatcgtg gcaacggcga
     1861 cgatcaccgt cgcaggcgaa ctccccgata tcccgcccac gaccacgacc acgaccgtgc
     1921 cgcccaccac caccatgccg accaccacca ccatgccgcc caccacagtg ccgaccacca
     1981 cagtaccgcc gaccacgcca tccaccacca cgaccatccc gaccacgacg acgaccgtgc
     2041 c
//
LOCUS       DAORNF010000644         2039 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_40812_length_2039_cov_1.650202, whole genome shotgun sequence.
ACCESSION   DAORNF010000644 DAORNF010000000
VERSION     DAORNF010000644.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40812_length_2039_cov_1.650202"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>212)
                     /locus_tag="PLV68_19285"
     CDS             complement(<1..>212)
                     /locus_tag="PLV68_19285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03449.1"
                     /translation="QAAFPEATCDDVFDNVALYSASITNFSRLTIDDGIFDSATIDQQ
                     MLTLSGSPTSGYTASLVVPIDASTAQT"
     assembly_gap    213..222
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<223..930)
                     /locus_tag="PLV68_19290"
     CDS             complement(<223..930)
                     /locus_tag="PLV68_19290"
                     /inference="COORDINATES: protein motif:HMM:NF012976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03450.1"
                     /translation="MPTAHHQPSNLGTERALPRPDEAIFDQGLQFDLGTLNRRRLLSL
                     FGIGAGAAALAACGSNSGAASSSTAATSTTTGAGTGGSTSTTSTAGATGTYVEIPDET
                     NGPYPADGTNGPDILEQSGVVRRDITTSFGDASGVAEGVPLTFSFTVIDIANGNVPWA
                     GAAVYAWHCDALGRYSMYSEGAENENYLRGVQVADDTGTVTFTSVFPACYTGRWPHIH
                     FEVYPDIDAIVDHDRCVS"
     gene            complement(1061..1567)
                     /locus_tag="PLV68_19295"
     CDS             complement(1061..1567)
                     /locus_tag="PLV68_19295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HRE03451.1"
                     /translation="MEAIERRVRDIVATMPGAEVIDCDPALADTADFCAAYGYSLDDS
                     ANAIVVIGKASSAVVAPVYAACVVLATTRLDVNRAVRQRLGTKKASFAPAEVTAALTG
                     MTMGGVTPFALPADLALWIDARVMQRDRIIVGGGSRAAKVVGPPSMLLTIAQAEVVTD
                     LAILLPTE"
     assembly_gap    1653..1683
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1684..>2039)
                     /locus_tag="PLV68_19300"
     CDS             complement(<1684..>2039)
                     /locus_tag="PLV68_19300"
                     /inference="COORDINATES: protein motif:HMM:NF016152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HRE03452.1"
                     /translation="AMSRTVTELALSMPGATLWPDREPPIYQVSGKSFVFFRTPRPDA
                     VDPESGERYDDVIVFWVGSDADKRALVEDPGSPFFTTPHFDGHSSVLVRACRIGEIDR
                     DELAEIVADAWLARAP"
BASE COUNT          311 a          636 c          726 g          325 t
ORIGIN      
        1 gtctgagcgg tgctggcgtc gatgggcacg acgagcgagg cggtgtagcc cgacgtgggg
       61 ctgccgctga gggtcagcat ctgctggtcg atcgtggccg agtcgaagat gccatcgtcg
      121 atggtcaacc gcgagaagtt ggtgatcgag gccgagtaga gcgctacgtt gtcgaacacg
      181 tcgtcgcagg tggcctcagg gaacgcggcc tgnnnnnnnn nncgacacgc accggtcgtg
      241 atcgacgatc gcgtcgatgt cgggatacac ctcgaagtgg atgtgcggcc agcggcccgt
      301 gtagcaggcg ggaaagaccg acgtgaaggt cacggtgccg gtgtcgtccg ctacctgcac
      361 gccgcgcaga tagttctcgt tctcggctcc ctcggagtac atcgagtagc ggccgagtgc
      421 atcgcagtgc catgcataga ccgcggcccc ggcccacggc acgttgccgt tggcgatgtc
      481 gatcacggtg aagctgaacg tcagcggtac gccttcggcc acgcctgatg cgtcgccgaa
      541 gctggtcgtg atgtcgcggc ggaccacgcc ggactgttcg aggatgtctg gcccgttggt
      601 gccgtcggct gggtacgggc cattggtctc gtcgggaatc tcgacgtagg tgccggttgc
      661 tccggcggtg ctggtcgtac tggtcgaacc gcccgtgcca gcgcccgtcg tggtgctggt
      721 cgccgcggtc gagctcgacg ccgcgcccga gttgctgccg cacgcggcga gcgcggccgc
      781 accggccccg ataccgaaca gcgacaacaa gcggcgacgg ttcagcgtgc cgaggtcgaa
      841 ctgaaggccc tggtcgaaga tcgcctcgtc cggtcgcggc aacgcgcgct cggtgccgag
      901 gttcgacggt tggtggtgag cagtgggcat cgtgtttctc ccggtgtggg gtcggtgtcg
      961 gaatcccgat ggaaccccgc actcgcagtg tcgtccccgc acctgagagc ctccgcagag
     1021 ttcctgcctg tttcctgcgt gtctgctgcg agcgcgcggg ctactcggtg gggaggagga
     1081 tggccaggtc ggtgacgacc tcggcctggg cgatggtcaa caacatcgac ggcggaccga
     1141 cgactttggc ggcacggctg ccgccaccga cgatgatgcg gtcgcgctgc atcacgcgcg
     1201 cgtcgatcca cagcgccagg tcggcgggca gcgcaaacgg ggtgaccccg cccatcgtca
     1261 tgccggtcag cgcggcggtc acctcggcgg gcgcgaagga tgccttcttg gtaccgagcc
     1321 gttggcggac ggcgcggttg acgtcgaggc gggtcgtcgc cagcacgacg caggcggcat
     1381 acacaggggc aacgacggcg ctggaggcct tgccgatcac cacgatcgcg ttggccgagt
     1441 cgtcgaggga gtacccgtag gccgcgcaga agtcggccgt gtcagccaga gctgggtcgc
     1501 agtcgatcac ctcggcgccg ggcatggtgg cgacgatgtc gcggacccgt cgttcgatcg
     1561 cctccatgga tcgccgacgc taccaacgcg gctcgtcgca ccagcggtca cctcggacgg
     1621 agacggtcac gccggtaggc cgcggttgtc gannnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnntcggcgc acgggcgagc catgcgtcgg cgacgatctc ggccagttcg tcgcggtcga
     1741 tctcgccgat gcggcaggca cgcaccagca ccgacgagtg accgtcgaag tgcggcgtcg
     1801 tgaagaacgg cgaccctggg tcctcgacca aggcgcgctt gtcggcgtcg gagccgaccc
     1861 agaacacgat cacgtcgtcg tagcgctcac ccgattcggg gtcgaccgcg tcaggcctgg
     1921 gcgtgcggaa gaacacgaac gacttgccgc tgacctgata gatcggcggt tcgcggtcag
     1981 gccacagggt cgcccccggc atcgacagcg ccaactccgt cacggtgcgc gacatggcg
//
LOCUS       DAORNF010000645         2038 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_40832_length_2038_cov_2.187595, whole genome shotgun sequence.
ACCESSION   DAORNF010000645 DAORNF010000000
VERSION     DAORNF010000645.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40832_length_2038_cov_2.187595"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            26..418
                     /locus_tag="PLV68_19305"
     CDS             26..418
                     /locus_tag="PLV68_19305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03453.1"
                     /translation="MNENAETARRQIQRMLEGTPFGPDDLADDAYDLVTVLLPRGQTA
                     LDAVSAAGLTAVGLPTTYPTTTDGERVGHAACWPAATDAYAAGLDGVHCRSAATLDGL
                     GRELAWWPRDQTATWTTRSPYSAWRSSP"
     gene            485..739
                     /locus_tag="PLV68_19310"
     CDS             485..739
                     /locus_tag="PLV68_19310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRE03454.1"
                     /translation="MTQPFNTLVGRDDVTARLGEITCPALVVHGTEDSAIEMERAEEL
                     AAGLSGCSGVVRVGGAHAANLTNPGPVNTAIVDFLNSLSA"
     gene            complement(1009..1749)
                     /locus_tag="PLV68_19315"
     CDS             complement(1009..1749)
                     /locus_tag="PLV68_19315"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HRE03455.1"
                     /translation="MTGTSLVLRTDRADGLTTLTLNRPEKLNALTPAVFVELRAHLDA
                     IAADSGVGCVVLAGAGRSFCAGHDLEAIADHQPAPSRHFEPETVDALEALPQPTIARI
                     QGHCFTGGLELALACDFLVSADTTRFGDTHGQWGLAPVWGMSVRLPERVGRSTAKQLM
                     FTSRRIDGTTAASIGLVDRCVPDDQLDAAVDALAVEILANSRGTNRIVKRLMADHADT
                     SRTAALIHERQLPHGLPDDMAARMSGRK"
BASE COUNT          356 a          721 c          661 g          300 t
ORIGIN      
        1 ggggcggaaa tagagatttg tccatatgaa cgagaacgcc gagaccgccc gccgacagat
       61 tcagcggatg ctggaaggga caccgttcgg tcctgacgac ctcgccgacg acgcctacga
      121 cctcgtgacg gtcctgctgc cccgcggcca gaccgctctg gacgcggtca gcgccgctgg
      181 gctcaccgcc gtcggcctgc ccactacgta cccaaccaca acggatgggg aacgcgtcgg
      241 acacgcagca tgctggcccg cagccaccga cgcctatgca gccggactcg acggggtgca
      301 ctgccgatcg gccgcaactc ttgacggcct cggccgcgag ttggcctggt ggccaagaga
      361 ccaaacggct acttggacca cccgttcccc ctacagcgcc tggcgctcga gtccctgagc
      421 ctgttcaact gcccggcccc ggtacggcgc gatcgccaag tggcgcgagc ggccgaacga
      481 ctacgtgacg cagccgttca acacgctggt cggacgcgac gacgtcaccg cccggttggg
      541 cgagatcacc tgccccgctc tcgtcgtgca tggcaccgaa gactccgcga tcgagatgga
      601 acgcgccgaa gaactggccg cgggtttgtc gggatgctcg ggcgtggtgc gcgtcggcgg
      661 cgcccacgcc gccaacctca ccaaccccgg ccccgtcaac accgccatcg tcgacttcct
      721 caactcgctg tcggcgtagc gcggccacca caagacaccc ggccaatccg aaaacacccg
      781 gccaatccga aagcacccgg ccaatccggc ttccgcggag tacggagtca ccggaatccg
      841 ggcaatcccc actccgcgga cgcccaatcc accgcaccgg gcaatccagc ttccacggac
      901 gggagattcg acatccggct tccgcggagt acggagtcac cggaaccccg gcaatcccca
      961 ctccgcggac ggtggatgca cccggcacgg gccgatgggt gagcgggact acttgcggcc
     1021 ggacatgcgg gcggccatgt catccggcag gccgtgcggg agttggcgtt cgtggatgag
     1081 cgcggcggta cggctggtgt cggcgtggtc tgccatcagc cgcttgacga tccggttggt
     1141 gccacgcgag ttggcgagaa tctcgaccgc gagcgcatcg accgcagcgt cgagttgatc
     1201 gtcgggtaca cagcgatcga ccaagccgat cgatgccgcc gtggtgccgt cgatgcgacg
     1261 gctggtgaac atcaactgct tggccgtcga acgccccacc cgctcgggta ggcggaccga
     1321 catgccccac accggtgcga gcccccattg gccgtgcgtg tcgccgaagc gggtcgtgtc
     1381 ggccgagacg aggaagtcgc aggccaacgc cagttccagt ccacccgtga agcagtgccc
     1441 ctggatgcgg gcgatcgtcg gctgcggcag cgcctccaac gcgtcgacgg tctccggttc
     1501 gaagtgccgt gacggcgccg gttgatgatc ggcaatcgct tccagatcgt gccccgcgca
     1561 gaacgagcgc ccggctccgg cgagcacgac gcacccgacg ccggagtcgg ccgcgatcgc
     1621 gtcgaggtga gcacgaagct cgacgaacac cgcaggcgtc agcgcgttga gcttctccgg
     1681 ccggttcagc gtgagcgtcg tcaatccatc agcgcggtcg gtgcgcaaca ccagcgatgt
     1741 accagtcatg gggcacaccg tagaacccgc tctccccgcc accgcagtcg tcccgacggt
     1801 accgcgaccc gctgccacgt catcgctgcg ctgaacgagc agtcgctctc agcagcgccg
     1861 tcccatgatc tcggagaggc gatccaccac gcgcggcaaa tccggcttcc gcggtgagtc
     1921 tggcttccgc ggagtacaga gttgccggtt tccgggcagt cctcactccg cggacgccga
     1981 attgggtgag tcccatcacg tggtgtagga gcgggccggc atcctcgggg tgatggtg
//
LOCUS       DAORNF010000646         2035 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_40900_length_2035_cov_1.501515, whole genome shotgun sequence.
ACCESSION   DAORNF010000646 DAORNF010000000
VERSION     DAORNF010000646.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40900_length_2035_cov_1.501515"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="PLV68_19320"
     CDS             complement(<1..252)
                     /locus_tag="PLV68_19320"
                     /inference="COORDINATES: protein motif:HMM:NF022112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HRE03456.1"
                     /translation="MRTAIPDGLEIRSAVLRADGTAEVDVTDELLSLSRSSLVDAVAQ
                     IVFTASEATGVRSVELKVDGQTVQWPAGDGELQRGPLSVY"
     gene            complement(589..>963)
                     /locus_tag="PLV68_19325"
     CDS             complement(589..>963)
                     /locus_tag="PLV68_19325"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRE03457.1"
                     /translation="SADRRRLARVIANLIDNARVHGGGDPEITVSEAAIDGEPIGHVS
                     IAVEDHGAGVPEEERSLVFERFARGAVAGRRSTSDGSGLGLALVDEHVRMHGGTVVVT
                     DRLDGDSGARFVIELPAEEVGV"
     assembly_gap    964..1077
                     /estimated_length=114
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1078..>2035)
                     /locus_tag="PLV68_19330"
     CDS             complement(<1078..>2035)
                     /locus_tag="PLV68_19330"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HRE03458.1"
                     /translation="GMETTGRLRRWWSLASDTLSRLAPHRIGLRSRILLTFSLGFLAL
                     AAFLAAITYSFTRSTLMRQRDRSAVEQSYRDASNALSRLRARPESAQDALDELAQLGI
                     QRPLLNYRDTWTGTPRFGADVLPDALVQEVVVQSNPARMIIRVEGVKMLVVGVPLPEV
                     GASYFEFFSLREVEDTLGSVGLSLLFAGIITTLFGAVLGSVSSRRAVRPLGGAAQAAK
                     AIAGGRLDTRLEVTDDAHLRVLADSFNDMAAALQARVERDARFASDVSHELRSPLMTL
                     AASVEVMNSRRDEIPERAQAALDLLAADVTRFQGLVEDLLEIS"
BASE COUNT          294 a          656 c          691 g          280 t
ORIGIN      
        1 gtacacgctc agtggcccgc gctgcagttc gccgtctccg gcgggccact gcacggtctg
       61 gccatcgact ttcagctcca cgctgcgcac gccggtcgct tccgatgcgg tgaacacgat
      121 ctgggccacg gcgtcgacca gcgagctgcg cgacaggctg agcagttcgt cggtgacgtc
      181 gacttcggcc gtgccgtcgg cgcgcaacac ggccgagcgg atctccaagc cgtcggggat
      241 cgcggtgcgc agccgcaggt agggcgtcgg tctcggcgac cgtgagcggg ccgaacagcg
      301 acgagagacg ctgtgtcacc gagtcgccga cgtcgcgcgc cgatgcgcgc agttgacggc
      361 tggacgtgct ggctccgggt gcgatcaggt agatgcggtc gctaccgctc gacgacgatg
      421 cctgtgcggc gggagcgacc agcaccggtc gttccgactc gacgatgggg cggggcatgt
      481 cgtcggtgct gatgccacac gccgtgacgg cagcgaccag cgcgagcatc gcccagcagc
      541 gcatcgtgcg acgcgctgcg tggggtgtgg tgtcgtgcat gccgcgcctc acacacccac
      601 ctcttccgct ggcagctcga tgacgaagcg ggcgccgctg tcgccgtcga gccgatcggt
      661 gaccacgacc gtgccgccgt gcatccgcac gtgttcgtcg accagcgcca ggccgagccc
      721 ggaaccgtcg ctggtgctgc gccgccccgc gaccgcgccg cgggcgaagc gttcgaacac
      781 cagcgatcgc tcttcttcgg gcacccctgc gccgtggtct tcgacggcaa tcgacacgtg
      841 gccgatcggt tcgccgtcga tcgcggcttc ggagacggtg atctccgggt cgccgccacc
      901 atggacgcgg gcgttgtcga tgaggttggc gatcacccgg gccaggcggc gccggtcggc
      961 gctnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnggc
     1081 tgatctcgag caggtcctcg accaggccct ggaaccgggt gacgtcggcg gcgagcaggt
     1141 cgagcgcggc ctgggcccgt tcggggatct cgtcacggcg tgagttcatc acctcgaccg
     1201 acgcggccag cgtcatcagc ggcgaacgca actcgtggct gacatcgctg gcaaagcgcg
     1261 cgtcgcgctc gacgcgagcc tgcagggcgg cggccatgtc gttgaacgaa tcggccagca
     1321 cgcgcaggtg ggcatcgtcg gtgacctcca agcgggtgtc gaggcggcca ccggcgatcg
     1381 ccttggcagc ctgggccgct ccgccgagag gacgcacggc gcgccgtgac gacaccgacc
     1441 cgagcacggc gccgaacagg gtggtgatga tgcccgcgaa cagcagtgac agcccgacgc
     1501 tgccgagggt gtcttcgacc tcgcgcaggc tgaagaactc gaagtacgac gcgccgacct
     1561 cgggcagcgg cacgccgacc accaacatct tcacgccctc cacgcggatg atcatccggg
     1621 ccgggttcga ctgcacgacc acttcttgca cgagcgcgtc gggcagcacg tcggcaccga
     1681 agcggggtgt gcccgtccag gtgtcgcggt agttgagcag cgggcgctgg atacccagct
     1741 gggccagttc gtcgagcgcg tcctgcgccg actcgggtcg ggcccgcagc cggctgagcg
     1801 cgttgctggc gtcgcgatag ctctgctcga cggccgagcg atcgcgctgg cgcatcagcg
     1861 tcgaacgggt gaacgagtag gtgatcgcgg cgaggaaggc cgccagcgcg agaaatccca
     1921 agctgaacgt caacaggatg cgactgcgca gcccgatgcg atgaggtgcc aggcgcgaca
     1981 gggtgtcgct ggcgagcgac caccatcgcc gcagccggcc ggtcgtctcc atgcc
//
LOCUS       DAORNF010000647         2034 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_40923_length_2034_cov_1.421425, whole genome shotgun sequence.
ACCESSION   DAORNF010000647 DAORNF010000000
VERSION     DAORNF010000647.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40923_length_2034_cov_1.421425"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>720)
                     /locus_tag="PLV68_19335"
     CDS             complement(<1..>720)
                     /locus_tag="PLV68_19335"
                     /inference="COORDINATES: protein motif:HMM:NF013570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HRE03459.1"
                     /translation="TRAASGGRVLAVVPAAGNGAGDDLVEIFLDRTPFYAEAGGQVGD
                     TGTISTDTGTAQVLDTTYAVPGLRKHTARLVQGEITPGQTAQAEIDVARRDAIRRNHT
                     GTHILHHALRSVLGPHVKQAGSLVGPDRLRFDFSHYSAVTDDEIAQIENLANSAVLAN
                     SPARVFETTKDEAEALGAIAFFGDKYGDIVRVLEVGNSIELCGGTHVRAAGDIGTIKI
                     VGESSIGSNLRRIEAVTGFNS"
     assembly_gap    721..903
                     /estimated_length=183
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<904..>2034)
                     /gene="alaS"
                     /locus_tag="PLV68_19340"
     CDS             complement(<904..>2034)
                     /gene="alaS"
                     /locus_tag="PLV68_19340"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HRE03460.1"
                     /translation="SLIPHDPTILFTVAGMVPFKPYFVGDETPPFKRAVTVQKCARAG
                     GKHNDLDDVGRTKRHLVFFEMMGNFSFGDYFKADAIPWSWELVTEVFGLDGDRIWITV
                     HNSDDEAEQIWHDVVGVPMDRIQRLGDKDNFWQMGETGPCGPCSELHIDRGPAFGPDG
                     GPLNDPQGDRFMEFWNLVFMQYNQANDGRRTPLPKPSIDTGAGLERILALVQGVDSIW
                     ETDVMLPLIDQAQSATGKRYLVGDYDDRDSCSLRVLAEHARSSTMLVNDGVFPSNEGR
                     GYVLRRIIRRAVRHAYLLGTEHLVMPGLVETAIEVMGNAYPDVAKNRDFIVGVLTKEE
                     ERFRQTLRTGLTILEDELVKGGAELSGRTAFTLHDTYGFPLEL"
BASE COUNT          285 a          610 c          625 g          331 t
ORIGIN      
        1 ccgagttgaa cccggtcacc gcctcgatgc ggcgcaggtt gctgccgatg ctgctctcgc
       61 cgacgatctt gatggtgccg atgtcacccg ctgcgcgcac gtgcgtaccg ccacacagct
      121 cgatcgagtt ccccacttcg agcacgcgca cgatgtcgcc gtacttgtcg ccgaagaagg
      181 cgatcgctcc caacgcctcg gcctcgtcct tggtggtctc gaacacgcgg gccgggctgt
      241 tggccagcac cgcgctgttg gcgaggttct cgatctgggc aatctcgtcg tcggtcacgg
      301 ccgagtaatg gctgaagtcg aaccgcaacc ggtcaggacc gaccagcgac ccagcctgtt
      361 tgacgtgcgg accgagcacc gagcgcaatg catggtggag gatgtgggta cccgtgtggt
      421 tacggcgaat ggcgtcacga cgggcgacgt cgatctcggc ctgcgcggtc tggccggggg
      481 tgatctcacc ctgcacgagc cgagcggtgt gcttgcgcaa cccgggcacc gcgtaggtgg
      541 tgtcgagcac ctgagcggtg ccggtgtcgg tcgagatcgt gcccgtgtcg ccgacctggc
      601 caccggcttc ggcgtagaac ggggtgcggt cgaggaagat ctcgaccagg tcatcacccg
      661 caccgttgcc cgcggcgggt accaccgcca gcacccggcc acccgaggca gcccgggtgt
      721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      901 nnngagctcc agagggaacc cgtaggtgtc gtggagggtg aacgcggtgc ggccgctgag
      961 ctcggccccg cccttgacca actcgtcttc gaggatcgtc aacccggtgc gcagcgtctg
     1021 gcggaagcgc tcttcctcct tggtcaacac cccgacgatg aagtcgcgat tcttggcgac
     1081 gtctgggtag gcgttgccca tcacctcgat cgcggtctcg accagaccgg gcatcaccag
     1141 gtgctcggtg ccgagcaggt aggcgtggcg caccgcgcga cggatgatgc ggcgcagcac
     1201 gtagccgcgg ccctcgttgc tggggaacac cccgtcgttg accagcatcg tgctgctgcg
     1261 agcgtgctcg gccaacaccc gcaggctgca gctgtcgcgg tcgtcgtagt cgccgaccag
     1321 gtaacgcttg cccgtcgccg actgggcctg gtcgatcagc ggcagcatca cgtcggtctc
     1381 ccagatgctg tcgacgccct gcaccagcgc caagatccgc tcgagaccgg caccggtgtc
     1441 gatgctgggc ttgggcagcg gcgtgcgacg accatcgttg gcctggttgt actgcatgaa
     1501 gaccaggttc cagaactcca tgaaccggtc gccctgcggg tcgttgagcg gcccgccgtc
     1561 gggaccgaat gccggcccgc ggtcgatgtg cagttcgctg cacggtccgc atgggccggt
     1621 ctcgcccatc tgccagaagt tgtccttgtc gccaaggcgc tggatgcgat ccatcggcac
     1681 cccgacgacg tcgtgccaga tctgctcggc ttcgtcgtcg ctgttgtgga cggtgatcca
     1741 gatgcggtcg ccgtcgaggc cgaacacctc ggtgacgagc tcccagctcc acgggatggc
     1801 gtcggccttg aagtagtcgc cgaagctgaa gttgcccatc atctcgaaga acaccaagtg
     1861 acgcttggtg cgcccgacgt cgtcgaggtc gttgtgcttg ccgcccgcgc gggcgcactt
     1921 ctgcacggtg accgcccgtt tgaacggagg tgtctcgtcg ccgacgaagt acggcttgaa
     1981 cggcaccatg cccgcgaccg tgaacaggat ggtcgggtcg tgcgggatca ggct
//
LOCUS       DAORNF010000648         2025 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_41140_length_2025_cov_1.221320, whole genome shotgun sequence.
ACCESSION   DAORNF010000648 DAORNF010000000
VERSION     DAORNF010000648.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41140_length_2025_cov_1.221320"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            139..>321
                     /locus_tag="PLV68_19345"
     CDS             139..>321
                     /locus_tag="PLV68_19345"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRE03461.1"
                     /translation="MGYEHLLVTTAGSVTTITLNRPEKRNALALNVMLELTEAFRHTA
                     ESDADGVVLAANGPVFS"
     assembly_gap    322..351
                     /estimated_length=30
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            459..>659
                     /locus_tag="PLV68_19350"
     CDS             459..>659
                     /locus_tag="PLV68_19350"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRE03462.1"
                     /translation="MATCDLAIAADTAGFALPGGKGGLFCHTPLVAVARNIGRKRAME
                     MALTGDPIDATTAADWGLINRAV"
     assembly_gap    660..822
                     /estimated_length=163
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1046..1227
                     /estimated_length=182
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1228..1645)
                     /locus_tag="PLV68_19355"
     CDS             complement(<1228..1645)
                     /locus_tag="PLV68_19355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03463.1"
                     /translation="MRTLRSWQLPGGPIESTADLLARCGYRLAPSTDSAPTKDARSAA
                     PTEDEDVIDPEDVCLAALIGLAAHQPLAARVVLQRIVPPLLGLANRKGRSPSHRDQLF
                     DDLVANAWMVILAYPAVRRPRRIAAGLVRDVSFQTFV"
     assembly_gap    1806..1819
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          259 a          525 c          597 g          255 t
ORIGIN      
        1 cgtgttgccg gcggtgcttg acgccgagca cgtcccgtgg cgcacgatcg agatctcctg
       61 agcgtcaccg ccaatacgtc acaggcagcc gagggtctcc gggcgttcgg gaggggtggg
      121 gtgcgcgaca ctgggaggat ggggtacgag cacctgttgg tcaccacggc cgggtcggtc
      181 acgacgatca cgctgaaccg tccggagaag cgcaacgcgc tggcactcaa cgtgatgctg
      241 gagctgaccg aggcgttccg gcacaccgcc gagagtgacg ccgacggtgt ggtgctggct
      301 gccaacgggc cggtcttcag cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nggcagttgt
      361 tcacggtgtg cacgcggatg atggacgcgg tccagtcgat gccgcagccg gtgatcgcca
      421 aggtccacgc cctggcgacg gcggctgggt gacaactggt ggcgacgtgt gatttggcga
      481 tcgcggccga cactgccggg ttcgccttgc cgggtggcaa gggcgggctg ttctgccata
      541 cgccgctggt cgcggttgct cgcaacatcg ggcgcaagcg ggcgatggag atggccctca
      601 ccggcgaccc gatcgacgcg accacggcag ccgactgggg actgatcaac cgtgccgttn
      661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnggccatga cacgcgacgc
      841 gcaagagggc ttcgccgcat ttctcggcaa gcgccacccc gacttcaccg agcgcttctg
      901 accggcctcg ctcggcgtcg cctcacgccg ccaccgcggc ggcagcgcgg aggcggtact
      961 cggcgcgggc gcgcatgttg cgcacggtgc gggcggtgac gccgagggcc tcggccatac
     1021 tctgcgaccc gccgtagttg accagnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnngga cgaaggtctg gaacgacacg tcacggacca
     1261 atcccgcggc gatgcgccgg gggcggcgca ccgctgggta ggcgaggatg accatccatg
     1321 cgttggcgac caggtcgtcg aacagttggt cccggtggct cggcgaacga cccttacggt
     1381 tggccaaccc gagcagtggc ggcacgatgc gttgcaacac cacacgcgcg gccagcggtt
     1441 ggtgtgccgc cagcccgatc agcgcggcca agcacacatc ctccggatcg atcacgtcct
     1501 cgtcctcggt tggggctgct gagcgcgcat ccttcgtcgg ggccgagtct gtggagggtg
     1561 cgaggcggta gccgcagcgg gcgagcaagt cggcggtgct ctcgatggga ccgccgggga
     1621 gctgccaact ccgcagcgtc cgcatggtgg cgatgtcgag gctgaggcac tgccactcgt
     1681 ggtcgagacg agccagggtc gaggtcgagt ggcgggggcg gtgcggtgag ccggccgcga
     1741 ggcgagttct ggccggaggg ccggaacgac tggtgccaga gcgagcagcg ccagtgggag
     1801 tgggtnnnnn nnnnnnnnng tcatgccggc caccgtgcgc ccgtgacctc acagcggcgc
     1861 tcacaggagc tctcaccggg ttgctcacaa tcgtcaagaa atcgtcaaga acacctggtt
     1921 gcagccggtt ttccttccgc tgacggctca ccggggcact cagggcgtca tgtctgaccc
     1981 gacgtcctgc ctactcgccg aaccatcaca tcgtttggca caccg
//
LOCUS       DAORNF010000649         2021 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_41239_length_2021_cov_1.292981, whole genome shotgun sequence.
ACCESSION   DAORNF010000649 DAORNF010000000
VERSION     DAORNF010000649.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41239_length_2021_cov_1.292981"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    216..372
                     /estimated_length=157
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <373..1135
                     /locus_tag="PLV68_19360"
     CDS             <373..1135
                     /locus_tag="PLV68_19360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03464.1"
                     /translation="RFAADDVAPLVAYLADPLPTTDLVVVSGGGRTAKALADAFKAAG
                     ATTVATAAPTNKRDRGTWIEDQVREAGLRLDARAVGALAAWMGEEPGRLPGVLDTLHS
                     VFGSDAKLTAADIEPFLGESGSVPPWDLTDAIDRGDTTTSLSMLARMSQSRHPLQIMA
                     TLHGHYVKMLKIDGADATSEAQVASVLGIKPGFPARKVLDQYRRLGGTGVSRAIGLLA
                     QADLDLRGQRDLPEDLVMEVLVARLSRLAPSPRRR"
     gene            complement(1202..1465)
                     /gene="rpsT"
                     /locus_tag="PLV68_19365"
     CDS             complement(1202..1465)
                     /gene="rpsT"
                     /locus_tag="PLV68_19365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HRE03465.1"
                     /translation="MANIKSQKKRNKTNAKRAARNKAVKSELRTRVKTAVSTIGTDDN
                     AESVRLAIKRLDMAAAKGVIHKNQAARRKSRLMKKINAANAAD"
     gene            1743..>2021
                     /locus_tag="PLV68_19370"
     CDS             1743..>2021
                     /locus_tag="PLV68_19370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03466.1"
                     /translation="MTSTSNSISRRIAALSCAALAVGTLAACGSDKKASTGPGSDFCK
                     TAQKLEDLNDDLDFQTATPKQIEAAIKETISLTKQAINHVEELQGKRTA"
BASE COUNT          283 a          628 c          634 g          319 t
ORIGIN      
        1 cacctgccgg cgctgcgcga gcgcgaggac gacgtcgtgc tgatcgcgcg ggcggtcgca
       61 ctgcaccgtc tcaacgcggg ggtggcgacg gatgcaccgg gcgcgggcgg acctgtcgcg
      121 ggggtcacgc ctgccgcggt ggtgccgcgc gacgcccgtg tgcaaaggtg accgccgtgt
      181 cgatccacct catcaccggc gacgacgagt cgctgnnnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nntcgcttcg ctgccgacga cgtcgctcca ctggtggcct atctggccga
      421 tccgctaccg accaccgatc tggtcgtggt cagcggtggt ggccgtaccg ccaaggccct
      481 ggccgacgcg ttcaaggccg ccggtgccac gaccgtggcc accgccgcgc cgaccaacaa
      541 gcgcgaccgc ggcacctgga tcgaagatca ggtccgcgag gcagggctgc gtctcgatgc
      601 ccgcgcggtc ggcgcgttgg cggcctggat gggggaggag ccgggacgac tgccgggcgt
      661 gctcgacacg ttgcactcgg tcttcggtag cgacgccaag ctgaccgcag ccgacatcga
      721 gccgttcctc ggtgagtcgg gcagcgtgcc cccgtgggat ctgaccgacg ccatcgaccg
      781 cggtgacacc accacctcgc tgagcatgct ggcccgcatg tcgcagagcc gccatcccct
      841 ccagatcatg gccaccctgc acggccacta tgtgaagatg ctcaagatcg acggggccga
      901 cgccaccagc gaagcgcagg tcgcgtcggt gctcggcatc aaaccgggat ttccggcacg
      961 caaggtgctc gaccagtacc gtcgcctggg cggcacgggc gtgtcccgcg cgatcgggct
     1021 gctggcccag gccgacctcg atctgcgcgg ccagcgcgac ctgcccgagg acctggtgat
     1081 ggaggtgctg gtggcgcgct tgtcgcgctt ggctccctca ccgcggcggc gctgacgcgc
     1141 aacgactcgg ctcggtcagc cgggtcgtca tgtcggcgcc ctgtcgggcg ctgagatcgg
     1201 ttcagtcggc cgcgttggcg gcgttgatct tcttcatcag gcggctctta cggcgagcag
     1261 cctggttctt gtggatgaca cccttggccg cggccatgtc gaggcgcttg atggccagac
     1321 gcaccgactc ggcgttgtcg tcggtgccga tggtgctgac cgcggtcttg acccgggtgc
     1381 gcaactcgct cttgacggcc ttgttgcgcg ccgcgcgctt ggcgttggtc ttgttgcgct
     1441 tcttctggct cttgatgttc gccacgtgcg aaatccttct ttgttgctga tggcccgggc
     1501 acgggccgac atgatcggtg ttggtgatct ggtcgttctg ccggggcaga acaggctcgg
     1561 cgatcctacc gccgcctggc gctgactccc acacgccgtg ctcgtccgcg atgtcgacgg
     1621 tgccgtgatg acggtgcagt gatggcgtcg tgagggtgca gtgatgactc tcggcacaac
     1681 gattgcgaaa accttggtca acaaccacga gatgcttcgc acggcgaata ttgtgcggcg
     1741 ccatgacatc gacttcgaac agcatctccc gccgcatcgc ggccctgtcc tgtgctgccc
     1801 tggcggtcgg caccctcgct gcgtgcggca gcgacaagaa ggcctcaacc ggccccggca
     1861 gcgatttctg caagaccgcc cagaagctgg aagacctcaa cgacgacctc gacttccaga
     1921 cggcgacccc gaagcagatc gaggccgcga tcaaagagac gatcagcctg accaagcagg
     1981 cgatcaacca tgtcgaggaa ctgcagggca agcgcacggc g
//
LOCUS       DAORNF010000650         2013 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_41441_length_2013_cov_1.541879, whole genome shotgun sequence.
ACCESSION   DAORNF010000650 DAORNF010000000
VERSION     DAORNF010000650.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41441_length_2013_cov_1.541879"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="PLV68_19375"
     CDS             <1..225
                     /locus_tag="PLV68_19375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03467.1"
                     /translation="GSSRAYSGAPGGSQSYNNYIKDTGVGSNCTGANDDYVNSSNEQA
                     AIRRTCKYTGATPTSTIAVLTSSAGRRVSH"
     assembly_gap    550..571
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<572..1421)
                     /locus_tag="PLV68_19380"
     CDS             complement(<572..1421)
                     /locus_tag="PLV68_19380"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRE03468.1"
                     /translation="MTSRRCRSVLFAASAVFSGLALIAACGDDSGSSGSGKVLKVYSG
                     RHYGIERAYELFEQQTGITVKFLNGDDAELRERIAAEGADTEADVYLTVDAGNLAAAA
                     DQGIFQPLDSEVLQRVIPAQLRDPDNLWFGLAVRYRTIVYTANEAWLPADEVPTTYEE
                     LADPKWKGRLCLRRSVDAYQQSLVASMIAADGYDATLETVRGWVDNAQILPNDVILID
                     TIAAGGCDVGIANHYYLARELAENPDLPVKLVWAEQDGRGVHVNVSGGGVTKYADDPE
                     LARQFLE"
     assembly_gap    1582..1615
                     /estimated_length=34
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          300 a          661 c          664 g          332 t
ORIGIN      
        1 ggttcgagcc gcgcctattc cggcgccccc ggtggctccc agagctacaa caactacatc
       61 aaggacaccg gcgtcggctc caactgcacc ggggccaacg acgactacgt gaactcatcg
      121 aacgagcagg cggccatccg gcgcacctgc aagtacactg gcgccacccc gaccagcacg
      181 atcgccgtgt tgaccagctc agccggtaga cgcgtgtccc actgatgtcg ccacaccgtg
      241 gtgttcgggt ggaccaagct gagcgccacc atcaccaccg gggcgacggc cagcgcggcc
      301 gcgatgacag ccggccacga ccatccccac cgcccgatcc ggtcgcgcca tcccaggcgt
      361 acggtggtcg gttcgatcgg gccggggtca gtcatacccg gcgacgtcca tcaggcggac
      421 ggcttccggg ttgagacgac cgacctcggc cgcgttcacc gtgtcgtgga tgaagccggc
      481 gccgaacgtg tcggtcagca actgctcggg cgcgacggca gggttggccg ggtactcgta
      541 gttgtcgccn nnnnnnnnnn nnnnnnnnnn nattcgagga actggcgggc cagttcgggg
      601 tcgtccgcgt acttcgtcac gccaccgccc gacacgttga cgtgcacgcc gcggccgtcc
      661 tgctcggccc acaccagctt caccggcagg tcggggttct cggccagctc gcgggcgagg
      721 tagtagtggt tggcgatccc gacgtcgcag ccgccggcgg cgatcgtgtc gatgaggatc
      781 acgtcgttgg gcaggatctg ggcgttgtcg acccagccgc gaaccgtctc gagggtggcg
      841 tcgtagccgt cggcggcgat catgctcgcg accagcgact gctggtaggc gtcgaccgac
      901 cggcgcaggc acagccgtcc cttccacttc ggatcggcga gctcttcgta ggtggtcggc
      961 acctcgtcgg cgggcagcca cgcttcgttg gcggtgtaca cgatcgtgcg gtagcgcacg
     1021 gccagcccga accacaagtt gtcggggtcg cggagctggg ccgggatcac gcgctgcagc
     1081 acctcggagt cgagtggctg gaagatgccc tggtcggccg ccgcggcgag gttgcccgcg
     1141 tcgacggtga ggtagacgtc ggcttcggtg tcggcacctt cggccgcgat ccgctcgcgt
     1201 agctcggcgt cgtcgccgtt caggaacttg accgtgatgc cggtctgctg ttcgaacagc
     1261 tcgtaggcgc gctcgatgcc gtagtgccgc ccgctgtaga ccttgagcac cttgccgctg
     1321 ccgctcgatc cggaatcgtc accgcacgca gcgatcaagg ccagaccgct gaacactgcc
     1381 gatgcggcga acagcaccga tcggcaccga cgagaggtca tgtcaaggga gcctatcgct
     1441 cgcttcaccc gtcatcccct gcgttgagcg tcccctgcgt ggagcgtccc ctgcgtggag
     1501 cgtcccatgc gtggagcgtc ccatgcgtgg agggctctgg tgcgctggcg ggtgcgatcg
     1561 cgatgccgac cctctatcgt gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngagtc
     1621 acaacgagcc gcgtcggttc gagttcaaac aagtcaagcc aacaaggagg tgtggatcga
     1681 tgagcgatgg cagtagtcac gtgcggtcgg gccgcacctt cacggcgtac cgcttcggct
     1741 gatctgcggc ggtgcggccc gaccgcgctg catctctgct gttcctcgtc gcttcaccga
     1801 tggtgagcgc accccgcggg tgtgctgcga tggaggtcag gatcatgagt gacgcgatcg
     1861 tcacagcccg gcacggccat ggggccatgc cactcatcta cgtcgcggac gtgtcgaagg
     1921 tgttcggcgc tccccgcgac ccagtcaccg tgctcaaccg ggtgtcgttg gaggtacgcc
     1981 gcggcgagat cgtcgcgctc ctcgccggcg cgc
//
LOCUS       DAORNF010000651         2009 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_41539_length_2009_cov_1.581372, whole genome shotgun sequence.
ACCESSION   DAORNF010000651 DAORNF010000000
VERSION     DAORNF010000651.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41539_length_2009_cov_1.581372"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            68..382
                     /gene="rpsJ"
                     /locus_tag="PLV68_19385"
     CDS             68..382
                     /gene="rpsJ"
                     /locus_tag="PLV68_19385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HRE03469.1"
                     /translation="MAQTIRIRIKAFDHEIIDQSTKKIVETVTRTSATIRGPIPLPTD
                     KHRYTVIRGPHIDKDSREHFEMRVHKRLIDIVDPNPKTIDSLQRIELPAGVDIEIKIQ
                     GS"
     gene            464..>1534
                     /locus_tag="PLV68_19390"
     CDS             464..>1534
                     /locus_tag="PLV68_19390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03470.1"
                     /translation="MTQGAVSEPAQTAQTAQIAAPGSPWGLRLAWPIWALAVLIALVH
                     LEAQHVPRTFFYDEWSFVLERRSGGIDTFLRPHNGHLSLIPVALYRIGFVLFGLDHYR
                     PYRVLALLVHAGVATALYWWVRQRLGWVIATAAGVVMLLIGSGWEVIFQPFAMNLSGA
                     VATALVAWIALERGTRRGDVVASIAAVAALCWSGVGLAAAGAMAVRLVVRREWRRLVV
                     AVVPAAAAYLVWTLAYGDSQVEMAGVRRAPRFVFDAAAAALAGLGARSLSLGRWVATV
                     VLVAVVVLVAVRTWRGGRAAFVACATRVLSCMPLIGACITWTLLAVGRATLSVPEQSR
                     YVYVGVALIVAAAADLVPRPAAA"
     assembly_gap    1535..1696
                     /estimated_length=162
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1711..>2009
                     /locus_tag="PLV68_19395"
     CDS             1711..>2009
                     /locus_tag="PLV68_19395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03471.1"
                     /translation="MLAEPYFQAIDDLGSPALSDGEMGLLSPFWAAEVDRVSIEIGWR
                     HPADITCAGADRQPWHEALGSAEIELAPSEIALVRAGAADVAVAARRLAPDPRPVT"
BASE COUNT          245 a          595 c          661 g          346 t
ORIGIN      
        1 atccgtgagg gtggccgtac tgtgggcgcc ggccgcgtca ccaagatcct caagtgagct
       61 gaacgacatg gcacaaacca tccgcatccg catcaaagcg ttcgatcacg agatcatcga
      121 ccagtcgacg aagaagatcg tcgagacggt cacgcgcacc tcggccacca tccgtggccc
      181 catcccgctg ccgaccgaca agcaccgtta tacggtcatc cgcgggccgc acatcgacaa
      241 ggactcccgt gagcacttcg agatgcgcgt ccataagcgc ctgatcgaca tcgtcgatcc
      301 caacccgaag accatcgaca gcctgcagcg catcgagctg ccggccggtg tcgacatcga
      361 gatcaagatc cagggcagct gagcccagac caggatctga acatgtgaga ggaggggccg
      421 gttcgccggc ccctcctgcg cgtgatcgat agcgttgccc gtcatgaccc agggagcagt
      481 gtcggagccg gcgcagaccg cgcagaccgc gcagatcgcg gcccctgggt ctccgtgggg
      541 gctccggctg gcgtggccga tctgggcgct ggcggtgttg atcgcgctgg tccacctaga
      601 ggcccagcac gttccgcgca ccttcttcta cgacgagtgg agcttcgtcc tcgaacggcg
      661 cagcggcggt atcgacacgt tcttgcgccc gcacaacggg cacctgtcgc tgatccccgt
      721 cgctctctac cgcatcggtt tcgtgctgtt cgggctcgac cactaccggc cctaccgggt
      781 cttggcgctg ctggtgcacg ccggcgtggc gacggcgttg tactggtggg tccggcagcg
      841 tctcggctgg gtgatcgcga cggcggccgg tgtcgtgatg ttgctgatcg gctcgggctg
      901 ggaggtgatc ttccagccgt ttgcgatgaa cctgtcgggt gcggtggcga ctgccttggt
      961 ggcgtggatc gcactggagc gcgggacgcg acgcggcgat gttgtcgcgt cgatcgctgc
     1021 cgtggcagcg ctgtgttgga gtggggtcgg ccttgccgcg gccggcgcga tggcagtgcg
     1081 gctggtggtt cggcgcgagt ggcgtcgcct ggttgtcgcg gtcgtcccag cggcggcggc
     1141 gtatctggtg tggacgcttg cgtatggcga ctcgcaggtc gaaatggcgg gtgtgcggcg
     1201 tgcgccgcgg ttcgtgttcg acgcggccgc tgcggctctt gccggcctgg gtgcgcggtc
     1261 gctgtcgctc ggtcgctggg tggcgaccgt tgtgctcgtc gctgtggtgg tgttggttgc
     1321 cgtgcgcacg tggcgcggtg ggcgagccgc gtttgtcgcg tgcgcaaccc gcgtgctgtc
     1381 gtgcatgccg ctcatcggcg cgtgcatcac gtggacgctg ctggccgtcg ggcgggccac
     1441 cctgtcggtg cccgagcaga gccgttatgt ctacgtcggt gttgcgctga ttgtggctgc
     1501 ggccgccgac ctcgtgccgc gacccgctgc cgctnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnatcg attgccggtc gtgttggctg agccgtactt ccaggcgatc
     1741 gacgacctcg gctcgcccgc gttgagcgat ggtgagatgg gcctactctc accgttctgg
     1801 gcggccgagg tcgatcgagt gtcgatcgag atcggctggc gacatccagc ggacatcacc
     1861 tgcgctggag cggaccgcca gccctggcac gaagccctcg gtagtgccga gatcgaactt
     1921 gccccgagtg agatcgcgct ggtccgcgcc ggggcggccg atgtcgccgt cgcggcacgg
     1981 cgcctcgccc ccgacccgcg accggtcac
//
LOCUS       DAORNF010000652         2007 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_41580_length_2007_cov_1.622951, whole genome shotgun sequence.
ACCESSION   DAORNF010000652 DAORNF010000000
VERSION     DAORNF010000652.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41580_length_2007_cov_1.622951"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="PLV68_19400"
     CDS             complement(<1..331)
                     /locus_tag="PLV68_19400"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03472.1"
                     /translation="MTIIGRVAASIDPGVTLTNRASVTADQLDSSATDNLSVASAIAT
                     AQSDLRVSVADSPDPVIAGADLTYSLVVVNQGPSEAPDVRLNAALPSGLTVRSIVPSQ
                     GDCGLSAD"
     assembly_gap    365..374
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<375..1419)
                     /locus_tag="PLV68_19405"
     CDS             complement(<375..1419)
                     /locus_tag="PLV68_19405"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03473.1"
                     /translation="MAADLVVADVITPTTGGVSLPVAITTTATMVGGVYELVFKAVGD
                     GLQDEAIVQLTVLEPRLAVTATPDAASLTTVGGEASITLSAAALHGATQPVELDLLDA
                     PVGLVYAFNNNLLGPGGSVTLALTDTAALANGVYTLNVRAEAGLRQDLIPLTLTVQKP
                     DFTLAPTLNRMAVIAGEEAAFAVGLSGANLTEPISFTLGSDSSPVPAGVVGFAETATR
                     APRPTLSLAAPGTAYFIVETDAETPAGPYRFDVLAAADGVSRREVFEVTVLAESVEAD
                     LGVGQTLAQTNVDVGGLITTTVRVVNYGPRTATTVTLTDTLPAGTTLVSATPSRGVCT
                     GAVDGAVCDLYALR"
     assembly_gap    1590..1769
                     /estimated_length=180
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1770..>2007)
                     /locus_tag="PLV68_19410"
     CDS             complement(<1770..>2007)
                     /locus_tag="PLV68_19410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03474.1"
                     /translation="YTGFPVAQAVATPRDGTPSGTALDLSGLASGQYWLWFSADDGRN
                     PPTRGYATTPIVVTHDWASEWIANLTTTPGFRSFA"
BASE COUNT          259 a          605 c          670 g          283 t
ORIGIN      
        1 cgtcggccga gaggccgcag tcgccttgtg agggcacgat cgagcgcacg gtcagcccgg
       61 aaggtagtgc cgcgttcagg cgaacgtcgg gcgcctccga ggggccttga ttgacgacga
      121 ccaggctgta ggtgaggtcg gcgccggcga tgaccgggtc ggggctgtcc gcgaccgaga
      181 cgcgcaggtc ggattgcgcc gtggcgatcg cgctggccac gctcaggttg tcggttgcgc
      241 tgctgtcgag ttgatcggct gtcacgctgg ctcggttggt cagcgtcacc cccgggtcga
      301 tgctcgccgc gacacgcccg atgatggtca cggcgacgat ccgggtcgag ccgcctgcca
      361 ggacnnnnnn nnnnggcgca gcgcgtatag gtcgcagacc gcgccgtcga ccgctccggt
      421 gcacacgccg cggctcggcg ttgcggaaac aagcgtggtg ccggccggta gggtgtcggt
      481 cagcgtgact gtcgtggccg tgcgcggtcc gtagttcacc acgcgcaccg tggtcgtgat
      541 caatccgccg acgtcgacgt tggtttgggc cagcgtttgc ccgacgccca gatcggcctc
      601 gaccgactcg gcgagcacgg tcacctcgaa gacctcgcgc ctgctgacgc cgtcggccgc
      661 ggcaaggacg tcgaaccggt atggcccggc gggcgtctca gcatcggtct cgacgatgaa
      721 gtacgcggtt cccggcgcag ccaggctgag cgtcggccgc ggcgcgcggg tcgccgtctc
      781 ggcaaacccg accacgccgg ccgggaccgg gctcgagtct gagccgagcg tgaacgagat
      841 cggctcggtg aggttcgcgc cactgaggcc gaccgcgaac gcggcctcct cgcccgcgat
      901 gacggccatc cgattgagcg tcggcgccag cgtgaagtcc ggtttctgga cggtcagcgt
      961 cagcgggatc agatcctgcc gcagcccggc ctcggcgcgc acattcagtg tgtacacgcc
     1021 gttcgccaaa gcagccgtat cggtcagggc cagcgtgacg ctgccgcctg ggccaagcag
     1081 gttgttgttg aaggcgtaca caaggccgac cggcgcgtcg agcaggtcga gctcgacggg
     1141 ctgtgtcgcg ccgtggagcg cggccgctga gagcgtgatc gacgcttcgc cgccgaccgt
     1201 cgtcaggctc gcggcatccg gcgtcgccgt gacggccagc cgcggctcga gcacggtcag
     1261 ttgaacgatc gcttcgtctt gcagcccgtc gccgacggcc ttgaagacaa gctcatagac
     1321 cccgccgacc atcgtcgccg tcgttgtgat ggccacgggc aggctcacgc cgcccgttgt
     1381 cggagtgatg acatcggcga ctacgagatc cgcggccacg ccatccggca cctgatcgac
     1441 gtagacgctg accgaggtcg gataggggtc gctcgtggtg gagaggtcaa gcgccacagt
     1501 ggctgtcgcg ccggcggtga cggtcagcgc tgccgtcggc gcgctgaggt cgaaggccgc
     1561 gccctgggcg acgaccgcga tcgtctcggn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnng gcgaagctgc ggaagcccgg cgtcgttgtc
     1801 aggttcgcga tccactccga cgcccagtcg tgggtcacca cgatcggcgt cgtcgcgtag
     1861 ccgcgggtcg gcgggttgcg cccatcgtcg gcgctgaacc agagccagta ctggccgctg
     1921 gccagcccgc tcaggtcgag cgcggttccg gagggcgtgc cgtcgcgtgg cgtggcgacg
     1981 gcctgggcca ccgggaaccc ggtgtaa
//
LOCUS       DAORNF010000653         2004 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_41654_length_2004_cov_1.894305, whole genome shotgun sequence.
ACCESSION   DAORNF010000653 DAORNF010000000
VERSION     DAORNF010000653.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41654_length_2004_cov_1.894305"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..368
                     /locus_tag="PLV68_19415"
     CDS             <1..368
                     /locus_tag="PLV68_19415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03475.1"
                     /translation="LPDNFELKLSILFLAAVVLGGPGNMGGVILGAVLVSYMPEKLRF
                     LNTSRYFWFGMVLMIMMIFRPQGLLPSMLKSRAIGNRDGPPPDGGDAIEPEEVDTVFH
                     DVSEGDPDLQGGAHGVAPA"
     gene            349..>855
                     /locus_tag="PLV68_19420"
     CDS             349..>855
                     /locus_tag="PLV68_19420"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRE03476.1"
                     /translation="MASHLLEIDHVTMQFGGLTALNDVDFHIDEGEILGLIGPNGAGK
                     TTCFNVVTGVYQATKGEVRFQGKSLAGIDRHRITKLGIARTFQNIRLFPDMSALENVM
                     VGADAHHRAGVVAAMLHLPQYKREQEEGYRRSYELLQFMGIADRADDAAKNLPYGYQR
                     RLEIARALA"
     assembly_gap    856..950
                     /estimated_length=95
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <951..1132
                     /locus_tag="PLV68_19425"
     CDS             <951..1132
                     /locus_tag="PLV68_19425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRE03477.1"
                     /translation="QGYTVLLIEHDMSLVMGVTDRIVVLDFGKKIAEGTPAEVRANPA
                     VVAAYLGESEDEGAA"
     gene            1122..1838
                     /locus_tag="PLV68_19430"
     CDS             1122..1838
                     /locus_tag="PLV68_19430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRE03478.1"
                     /translation="MLLEVENLHVYYGRIHAIKGISFTVGQGEIVTLIGANGAGKSTT
                     LKTISGVRPVTRGRVMFDGEDITHMPAHRRTEIGICQSPEGRGIFPGMTVMENLDMGT
                     YARKDRKSAARDQDLERVFDLFPRLAERRNQAGGSMSGGEQQMLAIGRALMSRPRVLL
                     LDEPSMGLAPMLIAQIFEIITEINHQGTTVLLVEQNAKQALKRAHRAYVLETGHVVKT
                     ADAHLLLDDPDVQAAYLGGH"
BASE COUNT          344 a          623 c          620 g          322 t
ORIGIN      
        1 ttctgcccga caacttcgag ctgaagctgt cgatcctgtt cttggccgcc gtggtgttgg
       61 gcgggcccgg caacatgggc ggcgtcatcc ttggtgcggt gttggtcagt tacatgcccg
      121 agaagctgcg cttcctcaac accagccgct acttctggtt cggcatggtg ttgatgatca
      181 tgatgatctt ccggccgcaa ggcttgctcc cgagcatgct gaagtcgcgc gccatcggca
      241 atcgcgacgg gccgccgccc gacggcggcg acgcgatcga accagaagaa gtcgacacgg
      301 tgttccacga cgtcagcgaa ggcgaccctg acctgcaagg aggcgcccat ggcgtcgcac
      361 ctgcttgaga tcgatcacgt cacgatgcag ttcggcggcc tcacagcctt gaacgacgtc
      421 gacttccaca tcgacgaggg cgagatcctc ggtctgatcg ggcccaacgg tgccggcaag
      481 acgacgtgct tcaatgtggt caccggggtg taccaggcga ccaagggtga ggtgcgcttc
      541 caaggcaagt cgctggcggg catcgaccgt caccgcatca ccaagctcgg catcgcccgc
      601 acgttccaga acatccgctt gtttccggac atgtcggcgt tggagaacgt gatggtcggc
      661 gccgacgccc accatcgcgc cggtgtcgtg gcggcaatgt tgcacctacc ccagtacaag
      721 cgcgagcagg aagagggcta ccgccggtcg tacgaactgc tgcagttcat gggcatcgcc
      781 gaccgtgccg acgatgccgc caagaacctg ccctacggct accaacgccg cctggagatc
      841 gctcgcgcct tggccnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn accagggtta
      961 caccgtgttg ttgatcgaac acgacatgag cttggtgatg ggtgtgaccg atcgcatcgt
     1021 ggtgctcgac ttcggcaaga agatcgccga gggcaccccg gccgaggttc gtgccaaccc
     1081 cgcggtcgtc gccgcctacc tcggagagtc ggaggacgaa ggtgctgctt gaagtcgaga
     1141 acctgcacgt ctactacggc cgcatccacg ccatcaaggg catctcgttc acggtcggcc
     1201 agggtgagat cgtcaccctc atcggcgcca acggggcagg caagagcacc acgctgaaga
     1261 ccatctccgg agtgcgtccc gtgacgcgtg gacgggtgat gttcgacggt gaggacatca
     1321 cccacatgcc ggcccaccgg cgcaccgaga tcggcatctg ccagtcgccc gaaggccgcg
     1381 gcatctttcc cggcatgacg gtgatggaga acctcgacat gggcacctat gcccgcaagg
     1441 accgcaagag cgcggcccgc gaccaggacc tggagcgtgt gttcgacctg ttcccgcgcc
     1501 tggccgaacg gcgcaaccag gccggtggtt cgatgtcggg tggcgagcag cagatgctcg
     1561 cgatcggacg tgcgttgatg tcgcggccgc gggtactgct gctcgacgag ccgtcgatgg
     1621 gcctggcacc gatgctgatc gcccagatct tcgagatcat caccgagatc aaccaccagg
     1681 gcacgaccgt gttgctggtc gaacagaacg ccaagcaggc gctcaagcgt gcccaccgcg
     1741 cgtacgtgct ggagacgggt cacgtcgtca agacggccga cgcccacctg ctgctcgacg
     1801 accccgacgt acaggccgcg tacctcggcg gccactgacc ggttgcacac agagtcgata
     1861 ccgcgtcaac ctgcaccgcg cactccgctc gcggcgacgg tggcggtgct ggttgcgtcg
     1921 ctgttgttcg gcacgaccgg caccgcccag gagctcggcc cggacgggct gacgccgttg
     1981 ggcatcggcg cggtgcggat tgtg
//
LOCUS       DAORNF010000654         2004 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_41659_length_2004_cov_1.613648, whole genome shotgun sequence.
ACCESSION   DAORNF010000654 DAORNF010000000
VERSION     DAORNF010000654.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41659_length_2004_cov_1.613648"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>390
                     /locus_tag="PLV68_19435"
     CDS             <1..>390
                     /locus_tag="PLV68_19435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03479.1"
                     /translation="IPYLEIRCSNTDDTGGWLANSTIYLGDDLPAGTFEDGEILLLFP
                     EDGRYEGSPPPSCEPPAVIAVDGTSLVLPLDADLIAAALSGTTTISGTAPETPPAAPS
                     TIVAPAPTTAVLSTDDRFGQVVAELAAA"
     assembly_gap    391..400
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <401..>684
                     /locus_tag="PLV68_19440"
     CDS             <401..>684
                     /locus_tag="PLV68_19440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03480.1"
                     /translation="DAPFTPPDDPNAMDLGIEPPSRIVNCTAGDVPIEIQQYASDAAL
                     RGLGLVGVALSEAFGVDPAQTFVVTAGDGVIAYGEAGEDIPASNEVTRRI"
     assembly_gap    685..796
                     /estimated_length=112
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(971..1360)
                     /locus_tag="PLV68_19445"
     CDS             complement(971..1360)
                     /locus_tag="PLV68_19445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03481.1"
                     /translation="MRTRSTYDPFGWRLQELADPDINPARRAELLDDLLVAEAEELAA
                     GRRHLTIVHGEPTDLADVEDLNEFYDTVSAIVENAEWFRLAGGKDYVTVTVAGARGDE
                     CLALAADAAHHRSTARWLIAEAPHPPC"
     gene            complement(1485..>2004)
                     /locus_tag="PLV68_19450"
     CDS             complement(1485..>2004)
                     /locus_tag="PLV68_19450"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRE03482.1"
                     /translation="PCLPGAGITPLDDDWAYPAPAELFANAPVDSPHHDYPAWDWGLP
                     VGTPLYAVRGGNVTTVQYWPHNWWDRGCTTTTQGCATCGIGVTITDTDDNRWVYCHGT
                     AVHVTAGDTVAAGAQILSSGNTGRSSGPHLHIQVRGADGVLRCPQSLLRSLRDQQAGL
                     DPAGLPTAGCYF"
BASE COUNT          278 a          600 c          695 g          309 t
ORIGIN      
        1 atcccgtacc tggagatccg ctgttcgaac accgacgaca ccggcggctg gctcgccaac
       61 agcaccatct atctcggcga tgacctgccg gccggcacgt tcgaggacgg cgagatcctg
      121 ctgttgttcc ccgaggatgg ccggtacgag ggcagcccgc cgccgtcatg cgaaccgccg
      181 gctgtgatcg cagtcgacgg caccagcctc gtcctcccgc tcgacgccga cctgatcgcc
      241 gccgcgctct ccgggacgac cacgatctcg ggcacggccc cggagacgcc gccggcggcc
      301 ccgtcaacaa tcgtcgcacc ggcaccgacg accgcggtgc tgagcaccga cgaccggttc
      361 ggtcaagtcg tcgccgaact cgccgcagcc nnnnnnnnnn gcgacgcccc gttcaccccg
      421 ccggacgacc cgaacgcaat ggacctcggt atcgagccgc cgtcacggat cgtgaactgc
      481 accgcaggcg atgtcccaat cgagatccag cagtacgcgt ccgatgcggc gttgcgcggt
      541 ctcgggctcg tgggcgtcgc cctttcggag gcgttcggcg tcgacccggc ccagacgttc
      601 gtcgtcacgg ccggcgatgg ggtgatcgcg tacggcgaag ctggcgaaga catcccggcg
      661 tcgaatgagg tgacccgccg tatcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnnnngcag gccaagggca gccatgcgtg cccgacgatc agccgccaac
      841 acggatcatg ccagaaggcc gggagagtgc atcacgacgc cggttacccg cggctctccg
      901 tagtgggcgg tggtaccgct gcacaggagc gcgcccccat cagaacggat ggcacgggac
      961 aactcctcct tcaacaaggt gggtgaggtg cttcggcgat cagccaacgg gctgtggagc
     1021 ggtggtgagc ggcgtccgca gcgagggcaa gacattcgtc cccgcgtgcc ccggcgacgg
     1081 tcacggtgac gtagtccttg ccacctgcaa ggcggaacca ctcggcgttc tcgacgatcg
     1141 cgctgaccgt gtcgtagaac tcgttgaggt cttcgacgtc ggcgaggtcg gtgggttcgc
     1201 cgtggacaat ggtgaggtgc cgtcggccgg cggcgagttc ctcggcttcg gcaacgagta
     1261 ggtcgtcgag tagttcggct cggcgggctg ggttgatgtc ggggtcggcg agctcttgga
     1321 gtcgccagcc gaaggggtcg taggtgctgc gggttcgcat ggctctcgtc cttgcgttgg
     1381 ggcccgcgcc ccacgagacg ggccaccagt tggagcggtg gagctgccat caagcgtagt
     1441 tcgctgccgt tctacggttc tgccggtcga cggccgccgg ccagtcagaa gtagcagccg
     1501 gccgtcggga gaccggcggg gtcgaggccg gcttgctggt cgcggagcga gcgcagcagc
     1561 gactgggggc agcgaagaac tccgtcggcg ccgcggacct ggatgtggag gtggggtccg
     1621 ctggatcggc cggtgttgcc tgagctgaga atctgggcgc cggctgcgac tgtgtcgccg
     1681 gcggtgacgt gtacggcggt gccgtggcag tacacccagc ggttgtcgtc ggtgtcggtg
     1741 atggtgacgc cgattccgca cgttgcgcag ccttgggttg ttgtggtgca gccgcggtcc
     1801 caccagttgt ggggccagta ctggacggtg gtgacgtttc cgccgcggac ggcgtagagg
     1861 ggggtgccga cgggcaggcc ccagtcccag gctgggtagt cgtggtgcgg gctgtcgacg
     1921 ggtgcgttgg cgaacagctc ggcgggtgcc gggtaggccc agtcatcgtc gagcggcgtg
     1981 atgcccgctc ctggaaggca cggg
//
LOCUS       DAORNF010000655         1990 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_42008_length_1990_cov_1.850129, whole genome shotgun sequence.
ACCESSION   DAORNF010000655 DAORNF010000000
VERSION     DAORNF010000655.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1990
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42008_length_1990_cov_1.850129"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="PLV68_19455"
     CDS             <1..339
                     /locus_tag="PLV68_19455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03483.1"
                     /translation="RQRSPPVLALSAHGFQMNVHLGGSLGVALPDNTVFVRFALNGVG
                     AQIDQGWEQRFVISDEEAKLCQSEAERVAFSSPGAPRRKPTAGTKPTAGTKAGGNAKA
                     ARSTASASRR"
     gene            complement(351..1061)
                     /locus_tag="PLV68_19460"
     CDS             complement(351..1061)
                     /locus_tag="PLV68_19460"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRE03484.1"
                     /translation="MTPRPARLLHTSDCHLDALDNGPCQRAFAASIDLAISTDVDAIV
                     ISGDLFDHARVAPDILDWTAMQLDRAERPVVLLTGNHDALHDTSVHHRFVAEQRCRAV
                     SMLDNPGGSSVEIPGTDVVVWGRAMVEHSPEYQPLLGSPSRVQGRWLVIAAHGLATAT
                     TAPGRSSVIPHTDLDALDADYVALGHIHAHQVVRNAPLAVYPGATARSFDGQAGCVIV
                     DLAPGRATTATWTALTWD"
     gene            complement(1058..1315)
                     /locus_tag="PLV68_19465"
     CDS             complement(1058..1315)
                     /locus_tag="PLV68_19465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03485.1"
                     /translation="MGREHDGGPRAWRTLHWPGGSQLVVVEPLAALDEVPGVHHVAFR
                     REDDAVLDLDTVLAAVPTTAPTFGTVIVIGSGQTIDRQPLP"
     assembly_gap    1581..1590
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1591..>1990
                     /locus_tag="PLV68_19470"
     CDS             <1591..>1990
                     /locus_tag="PLV68_19470"
                     /inference="COORDINATES: protein motif:HMM:NF037625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HRE03486.1"
                     /translation="IAERAGVTKRTLYGYFRSKDEAIVGADEDALGELAQGLRDRPAD
                     ERPGDALIAVLVGDDDPDGIMHRWLLRNELVARYPALLPRYLSWIVQIEVALTEALAE
                     RLGVDIAHDPAPRALVASTTAALRATLGWWW"
BASE COUNT          309 a          644 c          730 g          297 t
ORIGIN      
        1 cgtcagcgct ccccacccgt cctcgcgctc tcggcgcacg gcttccagat gaacgtccat
       61 ctcggcggtt cgctcggtgt ggccttgccc gacaacaccg tgttcgtccg cttcgcgctc
      121 aacggcgtcg gtgcacagat cgaccagggt tgggagcagc ggttcgtcat ctcggacgag
      181 gaagccaagc tgtgccagtc cgaggccgag cgggtcgcgt tctccagccc gggtgcgccg
      241 cgccgcaagc cgaccgctgg caccaagccg actgctggta ccaaggccgg tggcaacgcc
      301 aaggccgcgc gcagcacggc cagcgcttca cgccggtgat gggtcgctga tcaatcccag
      361 gtcagcgcgg tccaggttgc cgtcgtcgcc cggccgggcg cgaggtcgac gatgacgcat
      421 ccggcctggc cgtcgaacga ccgagccgtc gcgccgggat acaccgcgag cggtgcgttc
      481 cgcaccacct gatgtgcatg gatgtggccg agcgcgacgt aatcggcgtc gagcgcgtcg
      541 aggtcggtgt gtgggatcac agacgagcgc cctggggccg tggtcgctgt cgccagcccg
      601 tgggcggcga tgaccagcca gcgcccctgg acgcgggaag ggctgccgag cagcggttgg
      661 tattcggggg agtgctcgac catcgcccgg ccccacacca cgacgtcggt gccaggtatc
      721 tcgaccgacg acccacccgg gttgtcgagc atgctgaccg cgcggcagcg ctgctcggcc
      781 acgaaccgat ggtgcaccga cgtgtcgtgg agcgcgtcgt ggttgccggt cagcaacacg
      841 accggccgtt cggcgcggtc gagttgcatc gccgtccagt cgaggatgtc gggggcgacg
      901 cgggcatggt cgaagaggtc gccactgatc acgatggcgt cgacgtcggt ggagatcgcc
      961 aggtcgatcg acgccgcgaa cgcgcgctga cagggaccat tgtcgagcgc gtcgaggtgg
     1021 cagtccgacg tatgcagcag gcgagccggg cgtggcgtca tgggaggggc tggcgatcga
     1081 tcgtctggcc ggaaccgatc acgatcaccg tgccgaacgt cggtgcagtg gtggggacgg
     1141 cggcgagcac cgtatcgaga tcgagcacgg cgtcatcttc gcggcgaaag gccacgtgat
     1201 gcacgccggg cacctcgtcc agtgcggcca gcggttcgac gacgacgagc tgcgaaccgc
     1261 cgggccagtg cagggtacgc cacgctcgtg gtccgccgtc gtgttcgcgc cccaacctga
     1321 ttgtcgactt gaaaaagtgc gggtgtcgcg ttgcggaatc cgcaagtttg cgtagtctgc
     1381 aagtttgtga caagcggacc ccgaatggcc gaaccggtgc aggcggtcgc cgcgcaggat
     1441 gctccggctg cgacgggact ccgcgcggcg aaagcgcgcg caacgcgact ggcgctgtcg
     1501 caggcggcgc gggaactggt cctggagcgc gggctcgacg cggtgacggt cgacgacatc
     1561 gcggccgcgg ccggtgtgtc nnnnnnnnnn atcgccgaac gcgccggcgt caccaagcgc
     1621 acgctgtacg gctacttccg cagcaaggac gaagcgatcg tcggcgccga cgaggacgcc
     1681 ctgggcgaac tggcccaagg cctcagggat cgacccgctg acgaacggcc gggcgatgcc
     1741 ttgatcgccg tcttggtggg cgacgacgat cccgacggga tcatgcatcg ctggctgctg
     1801 cgcaacgagc tggtcgcccg ctacccggcg ctgctgccgc ggtacctgtc gtggatcgtg
     1861 cagatcgagg tggccctgac agaagcgctg gcagaacgac tcggggtcga catcgcacac
     1921 gatccggcgc cgcgggcgct ggtcgcgtcg acgacggcgg ccttgcgggc gacgttggga
     1981 tggtggtggg
//
LOCUS       DAORNF010000656         1990 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_42011_length_1990_cov_1.730233, whole genome shotgun sequence.
ACCESSION   DAORNF010000656 DAORNF010000000
VERSION     DAORNF010000656.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1990
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42011_length_1990_cov_1.730233"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>761)
                     /locus_tag="PLV68_19475"
     CDS             complement(<1..>761)
                     /locus_tag="PLV68_19475"
                     /inference="COORDINATES: protein motif:HMM:NF016944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HRE03487.1"
                     /translation="GFRDRIDPPYSGGSQLLSIARTDIDVDIHRHARPVCVAVRRFDN
                     LGHVVGEERFFGLFAAAAYRASVSTIPLVRESVSWVLGKSKFEPTSHSGRALRTVLET
                     FPRDVIFEIGRDELADVALEIVGLQERSLVRVIQIHSPENNWQTLAVYLPRNRLTPEM
                     PRRIAELIAQDTHAATMEFEPFVSTSALARVMVEIRRANPISNTHLDRLAEAIDTLTQ
                     SWHDRLADALDEAVGDSEAQRLMASIGATLPSDYA"
     assembly_gap    762..790
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<791..1590)
                     /locus_tag="PLV68_19480"
     CDS             complement(<791..1590)
                     /locus_tag="PLV68_19480"
                     /inference="COORDINATES: protein motif:HMM:NF016944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HRE03488.1"
                     /translation="MPNHHALEPGLRAVVDRATAHVGEPEQRLVADFTAAFFAEVDDD
                     ELAERAGELATLAVEHIRFGAVRRAGETLVEVRPSPDGKRSMLLVVNDDAPFLVDTIR
                     LALSRLGIEVFLMVHPGLDVERSPDGTLVDVGHEPSRLDNHLPLPGRVREAWTEMEVS
                     RLSADKAADVVEVVRAAVADVLAVVGDGEAMRLRAVALGEQLIEYPVIGHTATECEQV
                     GRLLVWLSRAHFVFLAAATYDVVDLEAPRSGSLIGGSEQQTLQVIDDSR"
     gene            1733..>1990
                     /locus_tag="PLV68_19485"
     CDS             1733..>1990
                     /locus_tag="PLV68_19485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03489.1"
                     /translation="MNEQPSQRGRSGAPGIDGAAGAFLPPAPTPGSWNALDPPGGSDL
                     IVNRPPPYVPPSNQRPGSGPKLLIVAAVVAGLALSITALVFL"
BASE COUNT          313 a          670 c          678 g          300 t
ORIGIN      
        1 cacgcgtagt cggacggcag cgtggcaccg atcgacgcca tcaaccgctg ggcttcgctg
       61 tcgccgacgg cctcgtccaa cgcgtcggca agacggtcgt gccagctctg ggtcagcgtg
      121 tcgatcgcct ccgcgagacg gtcgaggtgc gtgttgctga tcgggttggc gcggcggatc
      181 tcgaccatca ccctcgccag cgcggaggtg ctgacgaacg gctcgaactc catcgtcgcg
      241 gcgtgcgtgt cttgggcgat gagctcggcg atgcggcgcg gcatctcggg ggtgagccgg
      301 ttgcgcggca agtacaccgc caaggtctgc cagttgttct ccggcgagtg gatctggatc
      361 acccgtacca acgaacgctc ttgcaggccc acgatctcga gcgccacgtc ggcgagttcg
      421 tcgcgaccga tctcgaagat gacgtcgcgg gggaacgtct ccagcacggt gcgcagcgca
      481 cggccgctgt ggctggtggg ttcgaacttg ctcttgccga gcacccacga gacgctctcg
      541 cgcacgagcg ggatcgtgct caccgaagcc cgataggcgg ccgccgcgaa caggccgaag
      601 aaccgctcct cgccgacgac gtgaccgagg ttgtcgaagc ggcgcacggc cacacagacc
      661 gggcgagcgt ggcggtggat gtcgacgtcg atgtcggtgc gggcgatcga caagagctga
      721 ctgccgcccg agtagggcgg atcgatgcga tcgcggaacc cnnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn cggctgtcgt cgatgacctg caaggtctgc tgctcggacc cgccgatcag
      841 cgaaccgctg cgcggtgctt ccagatcgac cacgtcgtag gtcgccgccg cgaggaacac
      901 gaagtgggcc cggctcaacc acaccagcag gcgtccgacc tgctcgcact cggtcgcggt
      961 atgaccgatg accgggtact cgatcaactg ctcgccgagg gcgaccgcgc gcaggcgcat
     1021 cgcctcgccg tcgccgacca cggcaagcac atccgccacc gcggcacgga cgacctcgac
     1081 cacatcggcg gccttgtcgg cgctgaggcg tgacacctcc atctcggtcc atgcctcgcg
     1141 cacccggccg ggcaggggga gatggttgtc gagcctggat ggctcgtgac cgacgtcgac
     1201 cagcgtgcca tcggggctgc gctcgacgtc gagaccgggg tggaccatga ggaacacctc
     1261 gatgccgagg cgggagagag caaggcggat cgtgtcgacc aggaacgggg cgtcgtcgtt
     1321 gaccaccagc aacatcgagc gcttgccgtc gggtgacggg cggacctcga ccaacgtctc
     1381 ccccgcccgg cggacggcac cgaagcgaat gtgctcgacg gccaacgtgg ccaactcgcc
     1441 ggcccgctcg gccagctcgt cgtcgtcgac ctcggcgaag aatgccgcag tgaagtccgc
     1501 gaccaaccgt tgctcgggct cgccgacatg ggctgtcgcc cgatcgacga cggcgcgtag
     1561 gccaggctcg agtgcgtggt ggttgggcat gcgggcctac tttctgctcc ggcaacgctg
     1621 gcggaacacg acagggccgg gcacttcccg tcccctgcgc cgaacagtag tccgtcacct
     1681 gcccaggccg gggcgagccc ggtcgcgggt ggtagctgtt aatttcaaac aggtgaatga
     1741 gcagccgtcg caacgagggc gctcgggcgc ccccggaatc gacggtgccg cgggcgcatt
     1801 cctgccaccc gctcccaccc ctggttcatg gaacgcactc gatccacccg gcggttcgga
     1861 cctgatcgtc aaccggccgc cgccgtacgt cccgccgtcc aatcagcggc cggggtcggg
     1921 gccgaagctg ttgatcgtgg ccgccgtcgt cgccgggctg gcgctgtcga tcaccgcgct
     1981 ggtgttcctg
//
LOCUS       DAORNF010000657         1990 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_42013_length_1990_cov_1.513695, whole genome shotgun sequence.
ACCESSION   DAORNF010000657 DAORNF010000000
VERSION     DAORNF010000657.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1990
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42013_length_1990_cov_1.513695"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..647)
                     /locus_tag="PLV68_19490"
     CDS             complement(<1..647)
                     /locus_tag="PLV68_19490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HRE03490.1"
                     /translation="MLAHRTGSVAWLAVHLADFDYHLPEARIAQSALEPRDSARLLVD
                     QGDAAVPADRHVRDLTELLGDGDLLVVNDSRVIPARLAVHRQSGGAAEVLLLEPVASD
                     DGERRVWEALVRPARKLKVGEQLYGRSGTPIVEVGARTLAGDTFQVTLLGDGDPLAML
                     EREGDMPLPPYITTPLQQRSRYQTVYANEPGSAAAPTAGLHFTPELLARLEGQGVG"
     gene            655..1371
                     /locus_tag="PLV68_19495"
     CDS             655..1371
                     /locus_tag="PLV68_19495"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HRE03491.1"
                     /translation="MVTTRTDVVSLPSPDQPDAGMNLYLWVPDGGATAAILLIQEIFG
                     VGDYITAVAERLAAAGYLVGAPDVFWRFAPGFRVVHDEDGLNQAFAAVGQLDPPLAIG
                     DCTAALAHLAALDGIERTGVIGFCLGGTLAWGVAAAGDPDVCVSYYGSGVPDMIGMID
                     QVHCPTLFHFGNDDPYIANERVETINAAIAGREGFVLNVEHAGHAFDNHAAPMFHNES
                     AAKAAWAKTMAFLQMYLGPA"
     gene            complement(1396..>1990)
                     /locus_tag="PLV68_19500"
     CDS             complement(1396..>1990)
                     /locus_tag="PLV68_19500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Holliday junction DNA helicase RuvB C-terminal
                     domain-containing protein"
                     /protein_id="HRE03492.1"
                     /translation="TMLLPIRQAFDLYINLRPTKLLPGIPGPLRDRFGLVSRLDYYHD
                     DELELIVRRAAGILDVAISDTGAWEIARRSRGTPRIANRLLRRVRGDGAIDERTARTG
                     LQVFGVDERGLDKVDRAILTAICVQFRGGPVGLSTVAISVGEQTETVEDVYEPFLIQQ
                     GLLARTPRGRVAMPDAYHHLGLAAPSVTGERPPTLFS"
BASE COUNT          297 a          706 c          692 g          295 t
ORIGIN      
        1 cccacaccct ggccctccag acgggcgagc aactcgggcg tgaagtgcag cccggcggtg
       61 ggtgccgcgg ccgacccggg ctcgttggcg tacacggtct gatagcgcga ccgttgctgg
      121 agcggcgtgg tgatgtacgg cggcaacggc atgtcgccct cgcgctcgag catcgccaac
      181 ggatcaccgt cgccgagcag ggtcacctgg aacgtgtcgc cggccagcgt gcgcgcgccg
      241 acctcgacga tcggcgtgcc gctgcggccg tagagctgct cccccacctt cagcttgcgg
      301 gccgggcgca ccaacgcctc ccagacccgt cgttcgccgt cgtcgctggc gaccggttcg
      361 agcaacagca cctcggctgc gccgcccgac tggcggtgga ccgcgagccg ggcggggatc
      421 acgcgcgagt cgttgaccac cagcaggtcg ccgtcaccga gcagctcggt gaggtcacgc
      481 acatgacggt cggcgggtac ggcggcatcg ccctggtcga ccagcagccg cgcgctgtcg
      541 cgcggctcca gcgccgactg cgcgatgcgc gcctcgggca ggtggtagtc gaagtcggcc
      601 agatgcacgg cgagccacgc taccgagccg gtccggtgtg ccagcatggg cgccatggtc
      661 accacccgca cagacgtcgt ttcgctgccc agcccagatc agcccgatgc gggtatgaac
      721 ctgtatctgt gggtacccga cggcggggcg accgcggcga tcctgctgat ccaagagatc
      781 ttcggcgtcg gtgactacat caccgcggtt gccgagcgac tggcggcggc gggctacctg
      841 gtcggcgcgc ccgacgtgtt ctggcgcttc gctccgggct ttcgcgtggt ccacgacgag
      901 gacgggctca accaggcctt cgccgcggtc ggccaactcg acccaccgct ggcgatcggc
      961 gactgcaccg cagcgctggc ccacctggcc gcgctcgacg gcatcgaacg caccggtgtg
     1021 atcggcttct gtctcggcgg cacgctggcg tggggtgtgg ccgcagcagg tgatcccgac
     1081 gtgtgcgtca gctactacgg ctcgggcgtg ccagacatga tcggcatgat cgaccaggtg
     1141 cactgcccga cgctgttcca cttcggcaac gacgacccgt acatcgccaa cgagcgggtc
     1201 gagacgatca acgccgcgat cgccggtcgc gaagggttcg tgctcaacgt cgaacacgcc
     1261 gggcacgcgt tcgacaacca cgccgctccg atgttccaca acgagtcggc ggccaaggcc
     1321 gcctgggcca agacgatggc cttcctgcag atgtatctcg gaccggcctg aacagagcgc
     1381 tcgctgaacg gcgccttagc tgaacagcgt cggcggtcgc tcgccggtga ccgacggtgc
     1441 cgccaagccg aggtggtggt aggcgtcggg catcgctacc cggccgcgcg gcgtgcgggc
     1501 cagcagtccc tgctggatca ggaacggttc gtacacgtcc tcgaccgtct cggtctgctc
     1561 gccgacgctg atcgccaccg tcgacaggcc caccgggccg ccacggaact ggacgcagat
     1621 cgcggtgagg atcgcccggt cgaccttgtc gagtccccgc tcgtcgacgc cgaacacctg
     1681 caggcccgtg cgcgcggtgc gctcgtcgat cgcaccgtca ccgcgcaccc gccgcagcag
     1741 gcggttggcg atgcgcggcg tgccccggct gcggcgggcg atctcccacg ccccggtatc
     1801 gctgatggcg acatcgagga tgcccgcggc gcggcgcacg atcagctcca actcgtcgtc
     1861 gtggtagtag tcgagacgtg acaccagccc gaaccggtcg cgcagcgggc ccgggatgcc
     1921 cggcagcagc ttggtcgggc gcaggttgat gtacaggtcg aacgcctggc ggatcggcag
     1981 cagcatcgtc
//
LOCUS       DAORNF010000658         1987 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_42100_length_1987_cov_2.325569, whole genome shotgun sequence.
ACCESSION   DAORNF010000658 DAORNF010000000
VERSION     DAORNF010000658.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42100_length_1987_cov_2.325569"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..702)
                     /locus_tag="PLV68_19505"
     CDS             complement(<1..702)
                     /locus_tag="PLV68_19505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03493.1"
                     /translation="MNRLRRWATWMGAGLLAIATAAVAVPVVAPVPAVHAAGLGAGGE
                     YHPLTPDRIFDTRGTGINHTGGVIATSAAGGTVDVQILGQSGIPAAAAEVLAVAISIT
                     VTEPTQVGYLAAYPSGSAAGISSVVNFAKNQTVPNLSVQTVGANGKLTIKVVTPVSGT
                     AHVLIDVFGWFSTSAASQAGARLIPVGPARILDTREASFNPGAKALGERGVYTLPIRG
                     ADAQQPTITDVVPDSA"
     gene            complement(827..1033)
                     /locus_tag="PLV68_19510"
     CDS             complement(827..1033)
                     /locus_tag="PLV68_19510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HRE03494.1"
                     /translation="MAVDQRLLEILACPQDKGPLLYAESDAVLYNPRLRVAYPIRDDI
                     PVMLADEAEAVDEATHERLVAAAG"
     gene            complement(1059..>1987)
                     /gene="manB"
                     /locus_tag="PLV68_19515"
     CDS             complement(1059..>1987)
                     /gene="manB"
                     /locus_tag="PLV68_19515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799161.1"
                     /note="converts mannose-6-phosphate to
                     mannose-1-phosphate; the resulting product is then
                     converted to GDP-mannose by ManC which is then used in the
                     synthesis of mannose-containing glycoconjugates that are
                     important for mediating entry into host cells; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HRE03495.1"
                     /translation="SGIVLERHVQRGEAVQPGKALLTGFSLDELRVVADTANGMGGLV
                     VPAVFERLPLLDLEVMYGELDGTFPNHPADPLQPANQRDLQARVVSGGFDIGLAFDGD
                     ADRVFVVDEQGRGISGSTTTAMLASSVLRKHPGATILHNLICSRAVPEVIRECGGIPV
                     RTKVGHSFIKQIMADTGAVFGGEHSAHYYFLDNYRADSGLIASMLMLEEVSRGQQDLS
                     VMRKPFERYSSSGEINTQVDDPLAVIEMVADAFAGADQDRLDGLTVDSGAWWFNLRPS
                     NTEPLLRLNLEAPNRDECDQHVAELLSLITSV"
BASE COUNT          337 a          628 c          689 g          333 t
ORIGIN      
        1 ggcgctgtcg ggtacgacgt cggtgatcgt gggctgctgg gcgtcggcac cacggatcgg
       61 caacgtgtac acgccgcgct ctcccagcgc cttggcaccg gggttgaacg acgcctcgcg
      121 ggtgtcgagg atgcgggccg ggccgaccgg gatcagacgc gcccctgcct gggaagccgc
      181 gctggtggag aaccacccga acacgtcgat cagcacgtgg gcggtgccgg ataccggggt
      241 gacgaccttg atcgtcagct tgccgttggc acccacggtc tgcaccgaca ggttgggcac
      301 ggtttggttc ttggcgaagt tgacgacgct ggagatgccc gcagccgatc cgctggggta
      361 ggcggcgaga tagccgacct gggtcggttc ggtgaccgtg atgctgatcg ccacggccaa
      421 cacctcggct gcagcggcag ggatgccgct ctgaccgagg atctgcacgt cgacggtgcc
      481 gcccgcggcg ctggtggcga tgacgccgcc ggtgtggttg attcccgtgc cgcgcgtgtc
      541 gaagatgcga tccggggtca gggggtgata ctcgccacct gcgccgagac cggcagcgtg
      601 caccgccgga accggagcga cgacgggaac agcgaccgcc gcagttgcga tggcaagcaa
      661 tccggcaccc atccaagtcg cccagcggcg cagtctgttc attcggtcaa cgtttctctt
      721 cacaagtctc tcgcacatca gcccgctgag cgtgggagcg agcttaaccg tgggtagtca
      781 tcggcttccc gcgccccgaa gttgagacac ggatgagggg tacggttcat ccagcggcgg
      841 cgacgagacg ctcatgggtg gcctcgtcga ccgcttcggc ctcgtcggcc aacatcaccg
      901 ggatgtcgtc acggatcggg taggccacgc gcagacgtgg gttgtacaac accgcgtcgg
      961 actcggcgta cagcagtgga cccttgtctt gcgggcaagc gaggatctcc aacaggcgtt
     1021 ggtcgacagc catggtgtgc tccagaggtt cggccagctc agacgctggt gatcagcgat
     1081 aacagttcgg cgacgtgttg gtcgcactcg tcgcgattgg gcgcttcgag gttgagccgc
     1141 aacagcggtt cggtgttgga cgggcgcaag ttgaaccacc acgccccgct gtcgacggtg
     1201 aggccgtcga gccggtcttg atcggcaccg gcgaacgcat cggcgaccat ctcgatcacc
     1261 gccagcgggt cgtcgacctg ggtgttgatc tcaccgctcg acgagtagcg ctcgaacggc
     1321 ttgcgcatca ccgacagatc ctgctggccg cggctgactt cttcgagcat cagcatcgac
     1381 gcgatcaggc cgctgtcggc gcggtagttg tcgagaaagt agtagtgcgc cgaatgctcg
     1441 ccgccgaaca ccgccccggt gtcggccatg atctgcttga tgaacgagtg accgaccttg
     1501 gtgcgtaccg ggatgccacc gcactcgcgg atcacctcgg gcacggcgcg agagcagatc
     1561 agattgtgga ggatggtggc accggggtgc ttgcgcagca cgctggacgc gagcatcgcc
     1621 gtggtggtcg aaccgctgat gccacggccc tgctcgtcga cgacgaacac ccggtcggca
     1681 tcgccgtcga aggccagacc gatgtcgaag ccgcccgaga cgacccgggc ctgaaggtca
     1741 cgctggttgg caggctgcaa cgggtcggcc ggatggttcg ggaacgtgcc gtcgagctcg
     1801 ccgtacatca cctcgagatc gagcagcggc aggcgttcga acaccgcagg cacgaccagg
     1861 ccacccatgc cgttggcggt gtcggccacc acccgcagtt catcgagcga gaagccggtt
     1921 aacagagcct tgccaggctg gacggcttcg ccccgctgca catggcgctc caggacgatg
     1981 ccgctgt
//
LOCUS       DAORNF010000659         1987 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_42105_length_1987_cov_1.676501, whole genome shotgun sequence.
ACCESSION   DAORNF010000659 DAORNF010000000
VERSION     DAORNF010000659.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42105_length_1987_cov_1.676501"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            124..>345
                     /locus_tag="PLV68_19520"
     CDS             124..>345
                     /locus_tag="PLV68_19520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03496.1"
                     /translation="MVADPNTGEPIPVPVNYTELGNVADVTGGAFYEAPSADALEAAY
                     AEISDNLNAGVGDPIEVVTEQTWKFVAAAL"
     assembly_gap    346..410
                     /estimated_length=65
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(442..>743)
                     /locus_tag="PLV68_19525"
     CDS             complement(442..>743)
                     /locus_tag="PLV68_19525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HRE03497.1"
                     /translation="VGGMGARGKNFYNDICSAYGYEKEAVEVQDLYLDGKKDEAAKAV
                     PGEMLELSHLVGPRSYIKERIGAFKEAGVTVLSITPVGADPVKTVETLREIIDDA"
     assembly_gap    744..780
                     /estimated_length=37
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<781..1468)
                     /locus_tag="PLV68_19530"
     CDS             complement(<781..1468)
                     /locus_tag="PLV68_19530"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HRE03498.1"
                     /translation="MKISTMINYAGGFELEKVGLDQVMIAEAYSFDAVSQVGYLAAKT
                     ERVMIGTGILNVYSRTAALMAMTAAGCDYVSDGRFILGLGASGPQVIEGFHGVPYEKP
                     MVRIKEYIQACRMVWKREEPLNFQGQVVKAPLPAGEGTGLGKALKIINHPVRSEIPIW
                     WASLKDKSVEATAQYADGWLPIFFVPDRFQNVWGSSLKAGLAKRDPSLGQLQISAGGM
                     VAIGEDLVGEK"
     gene            complement(1566..>1987)
                     /locus_tag="PLV68_19535"
     CDS             complement(1566..>1987)
                     /locus_tag="PLV68_19535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03499.1"
                     /translation="LMDVVGPIAAAEGATVVLGVNLDDLGDHRPGQRAADERGAEFPM
                     VTAGLDKVAIRALSQRFGLRTWDKPAAACLASRIPYGTPVSVGILSRVDRAEAGLHRL
                     GFRQLRVRHYGDTARLEFDLDDLPAVLAQREQVVAIV"
BASE COUNT          313 a          641 c          613 g          318 t
ORIGIN      
        1 agcgtcacat cgtggagcac gaccccctct tcgtcgatgg atttcgaaaa cggtggcatg
       61 gaaccgggtt gtatgccgcc gatgtcggca tgatggccac gattggcgac gtaaaagagc
      121 ggagtggtcg ccgacccgaa caccggcgag cccatcccgg taccggtcaa ctacaccgaa
      181 ctcggcaacg ttgccgacgt gaccggtggt gcgttctacg aggcgccgag cgcggacgcg
      241 ctggaggcgg cctacgccga gatcagcgac aacctcaacg ccggggtcgg tgacccgatc
      301 gaggtcgtca ccgaacagac gtggaagttc gtcgccgccg cgttgnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn acagcgatgc
      421 ccgctccggc cgcagcccgg atcaggcgtc gtcgatgatc tcgcggaggg tctcgaccgt
      481 cttgaccgga tcggcgccga ccggggtgat cgacaacacg gtgacgccag cttccttgaa
      541 cgccccgatc cgctccttga tgtagctgcg cgggccgacc aggtgagaca gctcgagcat
      601 ctcgccgggc acggccttgg cggcttcgtc cttcttgccg tcgaggtaca agtcctgtac
      661 ttcgaccgct tccttctcgt agccgtaggc cgagcagatg tcgttgtaga agttcttgcc
      721 gcgcgccccc atgccaccga cgtnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      781 gcttctcgcc gaccaggtcc tcaccgatgg caaccatgcc cccggcagag atctgcaact
      841 ggccgagcga cggatcacgc ttggcgagtc cggccttgag cgagctgccc cacacgttct
      901 ggaaccggtc gggaacgaag aagatcggca gccagccatc ggcgtactgg gccgtggcct
      961 cgacgctctt gtccttgagg ctggcccacc agatcgggat ctcgctgcgc accgggtggt
     1021 tgatgatctt cagcgccttg cccaatccgg tgccctcgcc cgctggcagc ggagccttga
     1081 ccacctggcc ctggaagttg agcggctctt cccgcttcca caccatgcgg caggcctgga
     1141 tgtactcctt gatgcgcacc atcggcttct cgtagggcac gccgtggaag ccttcgatca
     1201 cctgcgggcc gctggcgccg aggccgagga tgaaccgtcc gtcgctgacg tagtcgcaac
     1261 ccgcggcagt catggccatg agcgctgcgg tgcggctgta gacgttgagg atgccggtgc
     1321 cgatcatgac ccgctcggtc ttggccgcca ggtagccgac ctggctgacc gcgtcgaagc
     1381 tgtacgcctc ggcgatcatc acctggtcga ggcccacctt ttcgagctcg aacccaccgg
     1441 cgtaattgat catcgtggag atcttcatga gggcgaccct acacggggtc cctcccggga
     1501 ctgaggttga ggttgccgga ccggaaccct tcgagatcga gggtcaccgc ccggtaccca
     1561 gcggcctaca cgatcgcgac cacctgctcg cgttgagcga gcactgcggg caggtcgtcg
     1621 aggtcgaact ccagccgggc ggtgtcgccg tagtggcgca cccgcagttg acgaaagccc
     1681 aggcgatgca ggccggcttc ggcccggtcg acccgcgaca gaatgcccac cgagaccgga
     1741 gtcccatagg ggatgcggct ggccaggcag gccgcggcgg gcttgtccca ggtgcgcagg
     1801 ccgaaccgct gcgacagggc acggatcgcc accttgtcca acccggcggt caccatcggg
     1861 aactcggctc cccgctcgtc cgcggcacgc tggccggggc gatggtcgcc gagatcgtcg
     1921 agattgacgc ccagcaccac cgtggcgcct tcggccgcgg cgatcgggcc gaccacgtcc
     1981 atcagct
//
LOCUS       DAORNF010000660         1982 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_42242_length_1982_cov_2.247535, whole genome shotgun sequence.
ACCESSION   DAORNF010000660 DAORNF010000000
VERSION     DAORNF010000660.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1982
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42242_length_1982_cov_2.247535"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(409..828)
                     /locus_tag="PLV68_19540"
     CDS             complement(409..828)
                     /locus_tag="PLV68_19540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03500.1"
                     /translation="MAHAVKGIVAKGKGLPVELTTILSLKASGHLGDISSGALYFQNA
                     TIVAARAATGTVAPSLVHFGGMPQTAVIGRHTFYRPAAETSIKAAGTATPARPALRSS
                     FVGGDPGTDRATPEASQGETVLVLPDAVSVAEVARAP"
     gene            930..1778
                     /locus_tag="PLV68_19545"
     CDS             930..1778
                     /locus_tag="PLV68_19545"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRE03501.1"
                     /translation="MAMARTADGVEIWYDTIGAGRDVVLVHGITDSSGTWGEITPALA
                     EHYRVTTLDLRGHGHSGDAPSYEAVQMAADVAAVVDAAGLVEPLLVGHSLGGIVVTAY
                     AASSPLASRGVVNVDQPLQLSAFQAGLAPAEGLLRDPASFGPVLSAVFESMDGSGMTA
                     AVREHITAHRNPRQDVVLGVWDAVFTQSVAQLDELVATMIGRLSAPYLALQYMPAPDG
                     YDDWLRALIPQAVVETWPDGTGHYEHLLDPGAFVARLTAFDAQPPITETRSLGSFSDG
                     RGRRGS"
     gene            complement(1747..>1982)
                     /locus_tag="PLV68_19550"
     CDS             complement(1747..>1982)
                     /locus_tag="PLV68_19550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HRE03502.1"
                     /translation="QRLTRLAVGLLDDDGVLVQSSCSSRVGADDFFAAVHAAAAGSGV
                     RLVDIVRTGHALDHPTTFAQSAYLKTLFARVHR"
BASE COUNT          301 a          680 c          686 g          315 t
ORIGIN      
        1 agtcgaccgt ctgcccgtcg tggccgtgca gccgctcgcc ggcgaggtag gccgccttgc
       61 cgatggcacc gcgcgtcgtc agccggtggc cgccgtggtg ggcgctgcgc gcgatcgtcg
      121 tgcccgcctt cttcaccgga gtgagccgga gatgatagat cgccacgact cacctgttct
      181 cgtttaggtt gtaggccgcc gggcatgtta cagcccggca ccccgccggg gtactaagtg
      241 cgcagtaatc ttcatttgat ttcagtgact tggaaaatct ggacagcggc ggcgactggc
      301 tggacaggtt cgggctcagg cacttagccg aatcttggac agggttggac aacggcctgt
      361 ccagcggcgc acgggcggca atccaaccac gacgaacctc aggcggtgtc atggcgccct
      421 cgcaacctct gcaaccgaca cggcatcggg aaggaccagc acggtctcgc cctggctggc
      481 ctcgggtgtc gcgcggtcgg tccccggatc accgccgacg aagctgctgc gcagcgccgg
      541 acgcgccggg gtcgcagtgc ccgctgcctt gatggaggtc tcggcggcag gccggtagaa
      601 ggtgtgtcgg ccgattaccg ccgtctgcgg catgccgccg aagtggacga gcgacggcgc
      661 aaccgtgccg gtcgccgctc gcgccgcgac gatcgtcgcg ttctggaagt acaacgcacc
      721 gctggaaata tcgccgagat ggccactggc cttgaggctg aggatcgtcg tcagctcgac
      781 gggcaatccc ttgcccttgg cgacgattcc cttcactgca tgtgccatcg gatgctccct
      841 cgtggtgccg gcgcgacgcc gggatcgaac gtggcgacct taccggctcc cacccacccc
      901 acccagagcc agcgcggtag cgttcgccca tggcgatggc gcgcacggcc gacggggtgg
      961 agatctggta cgacacgatc ggggccggcc gtgatgtcgt gctggtccac ggcatcacgg
     1021 acagttccgg cacgtggggg gagatcacgc ccgcactggc cgagcactac cgggtcacca
     1081 cgctcgacct gcgcggtcac gggcactcgg gcgacgcgcc gagctacgag gcggtgcaga
     1141 tggccgcgga cgttgccgca gtggtcgacg cggcggggct cgtcgagccg ctgctggtcg
     1201 gccattcgct cggcggcatc gtggtgaccg cctacgcagc ctccagtccg ctcgccagtc
     1261 gcggcgtcgt caacgtcgac cagccgttgc aactcagcgc gttccaggct gggctcgcac
     1321 ccgctgaggg attgctgcgc gacccggcct cgttcggccc ggtgttgagc gctgtgttcg
     1381 agtcgatgga cggcagcggc atgaccgccg cggtccgcga gcacatcacg gcgcaccgca
     1441 acccacgcca agacgtcgtg ctgggtgtgt gggatgcggt gttcacccag tccgtcgcgc
     1501 agctcgacga actggtcgcc accatgatcg gccgtctgtc agcgccgtac ctggcactcc
     1561 agtacatgcc agcacccgat ggctacgacg actggctgcg ggcgctgatc ccccaggccg
     1621 tggtcgaaac ctggccggac ggcaccggcc attacgagca cctgctcgac cccggcgcct
     1681 tcgtcgcccg cctcaccgcc ttcgacgccc agccaccgat caccgagacc cgatcgctcg
     1741 gctcgttcag cgatggacgc gggcgaagag ggtcttgagg taggcgctct gggcaaaggt
     1801 ggtggggtgg tcgagcgcgt ggccggtgcg cacgatgtcg accaggcgca caccgctgcc
     1861 ggccgcggcg gcgtggaccg cggcaaagaa gtcgtccgcg ccgacccggc tggaacacga
     1921 gctctgcacg agcacgccgt cgtcgtcgag cagcccgacg gccagacggg tgagccgctg
     1981 gt
//
LOCUS       DAORNF010000661         1978 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_42353_length_1978_cov_1.439938, whole genome shotgun sequence.
ACCESSION   DAORNF010000661 DAORNF010000000
VERSION     DAORNF010000661.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1978
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42353_length_1978_cov_1.439938"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="PLV68_19555"
     CDS             <1..573
                     /locus_tag="PLV68_19555"
                     /inference="COORDINATES: protein motif:HMM:NF039222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03503.1"
                     /translation="AVYRDGTLNLMALPDDLLASIDTKLPPRWSRNNPVDCAGGETRD
                     TIPEVMEMIAAHPDVHSMIYIGLGIQSNQARLMAEGKFYPDHGLERIVAYHRRQDERF
                     AEAASAYQKLADLYPGSLYATQMREKVRTTEAKPILCATELAVADPDNPGPAAVRASG
                     RLCYPSGNRAVTALGHLYRYAEFRRTRGLA"
     gene            624..>922
                     /locus_tag="PLV68_19560"
     CDS             624..>922
                     /locus_tag="PLV68_19560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03504.1"
                     /translation="MVLLALLPTVGFAAAWRWADDKAKAEEQPPVVSYAPPPGAGVTA
                     LDAPVLSVRRAPSALVESTSEAALVNELVNFAGFLNDTSCLAVSLSGRTVFTHNA"
     assembly_gap    923..932
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <933..>1329
                     /locus_tag="PLV68_19565"
     CDS             <933..>1329
                     /locus_tag="PLV68_19565"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HRE03505.1"
                     /translation="PASTMKLIVGAVALEVLGDDYAFVTRLYGSVADGVVQGDLVMVG
                     GGDPVLSTADYPETQPIPPTVTTSLEALVANLAAAGVTRINGGIVVDESRYDTERYAP
                     GWGADIRDVEAGPLSALMVNDAIRQVGVGN"
     assembly_gap    1330..1365
                     /estimated_length=36
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1366..>1978
                     /gene="dacB"
                     /locus_tag="PLV68_19570"
     CDS             <1366..>1978
                     /gene="dacB"
                     /locus_tag="PLV68_19570"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HRE03506.1"
                     /translation="AGTALASLLTEAGIQLVGGVRTGALEIGVPEIASVTSPPLSAIV
                     ADMLTISDDNTAELLLKEIAVATELGDDRQAGLDAVVATLTTWGIDTSTLTLADGSGL
                     SSTNQVTCDVLLAVLDRQPADGPLRAGLPIAGQTGTLAAETAFDESVIGRLHAKTGTL
                     TGVKALAGFVTTGGGELDFALILNGDGIKDVYQPLWGRLGQIAA"
BASE COUNT          301 a          691 c          652 g          288 t
ORIGIN      
        1 gctgtgtacc gcgacggcac gctgaacctg atggccttac ccgacgatct gctggcgtcg
       61 atcgacacca agctgccgcc gcgttggagc cgcaacaacc cggtcgactg cgccggtggc
      121 gagacgcgtg acacgattcc cgaggtgatg gagatgatcg cggcgcatcc cgacgtgcac
      181 tcgatgatct acatcggcct cggcatccaa tcgaaccaag cccgcctgat ggccgagggg
      241 aagttctatc ccgaccacgg tctggaacgg atcgtcgcct accaccgccg tcaggacgag
      301 cgcttcgccg aagcggcttc cgcataccaa aagctggccg acttgtaccc cggctcgctg
      361 tatgccacgc aaatgcgcga gaaggtgaga acgaccgagg ccaagccgat cctgtgcgcg
      421 accgaactgg ccgtcgccga ccccgacaac cctggccccg ccgccgtgcg cgccagcggg
      481 cggttgtgct acccgtcggg caaccgggcg gtcaccgcgc tcggccacct gtaccggtac
      541 gccgagttcc gtcgcacgcg aggtctggct tgaactatcg gatggcggta cccccgcgcc
      601 cgtcgaggcg ccccgtggtc gtcatggtgc tgctcgcatt gctgccgacc gtcggcttcg
      661 cggccgcgtg gcgctgggcc gacgacaagg ccaaggccga agagcagccg ccggtcgtgt
      721 cgtatgcacc gccgcctggg gccggtgtga ccgccctcga cgcgcccgtg ctgtcggtgc
      781 gccgcgcgcc cagcgcgctg gtggaatcga ccagcgaagc agcgctggtg aacgagttgg
      841 tcaacttcgc cgggttcctc aacgacacgt cgtgcctggc cgtgtcgctg tcgggccgca
      901 cggtgttcac ccacaacgcc gannnnnnnn nnccccgcca gcaccatgaa gctgatcgtc
      961 ggtgccgttg cactggaggt cctcggcgac gactacgcgt tcgtcacccg cctgtacggc
     1021 agcgtcgccg atggcgtggt gcagggcgac ctggtgatgg tcggcggcgg cgacccggtg
     1081 ttgtccacgg ccgactaccc cgagactcag ccgatcccgc cgacggtcac cacgtcgctg
     1141 gaagcgttgg tcgccaacct ggccgcggcc ggggtgaccc gcatcaacgg cggcatcgtc
     1201 gtcgacgagt cgcgctacga caccgagcgc tacgcgcccg gctggggtgc cgacatccgc
     1261 gacgtcgaag cgggaccgct gtcggcgctg atggtcaacg atgcgatccg ccaggtgggg
     1321 gtgggcaacn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngcggg caccgcgctc
     1381 gcgtccctgc tgaccgaagc cggcatccaa ctggtcggcg gcgtgcgcac cggcgcactc
     1441 gagatcgggg tgcccgagat cgcctcggtc acctcgccgc cactgtcggc catcgtcgcc
     1501 gacatgctca ccatcagcga cgacaacacc gccgagttgt tgttgaagga gatcgcggtc
     1561 gcgaccgaac tgggcgacga tcgtcaagcg ggcctcgacg cggtcgtggc caccttgacc
     1621 acctggggca tcgacacctc gacgctcacg ctggccgacg gctcgggcct gtcgtccacc
     1681 aaccaggtga cctgtgacgt gctgctcgcc gtactcgacc gccaacccgc cgacgggccg
     1741 ctgcgggccg ggctgcccat cgccggccag accggcaccc tcgccgcgga gacggccttc
     1801 gacgagtcgg tgatcgggcg cctgcacgcc aagaccggca cgctcaccgg cgtcaaggcg
     1861 ttggccgggt tcgtcaccac gggtggggga gagctcgact tcgcgctgat cctcaacggc
     1921 gacggcatca aggacgtgta ccagccgctg tgggggcgcc tgggccagat cgccgcgg
//
LOCUS       DAORNF010000662         1972 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_42500_length_1972_cov_1.423057, whole genome shotgun sequence.
ACCESSION   DAORNF010000662 DAORNF010000000
VERSION     DAORNF010000662.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1972
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42500_length_1972_cov_1.423057"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            266..>1624
                     /locus_tag="PLV68_19575"
     CDS             266..>1624
                     /locus_tag="PLV68_19575"
                     /inference="COORDINATES: protein motif:HMM:TIGR03599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HRE03507.1"
                     /translation="MVAFSTAMQAHAADINRLNVYPVPDGDTGTNMSRTLAAVVAELD
                     AAELDRAGLSAAGADMASICTAISHGSLMGARGNSGVILSQILRGLASVLRDLDEHGA
                     DAAQFATALTTASAAAYQAVLKPIEGTILTVVREASEAATAAAADGASLADTIRAARH
                     QGGDALARTPDLLPVLRDAGVVDAGGAGFLLLLDAALHVVAGDDMPEPSDLADGAADV
                     PQPFTPRSGHDGELDVSEQRYEVMYFLDLADEQIGAFKQAWGDLGDSIVVVGGDGLWN
                     CHVHTNDIGAALEAALDLGGRPRQIRVTDLFAEVADQHAAREAAIHQPAHTSLIGASH
                     VHPGINQPAVTCAIVAVSSGQGLTELFANLGVQGVISGGQTLNPSTAELLDAVEAVNS
                     DQVVVLPNNKNIIPVAEQLDALTHKTVVVVPTVSMPEALAALIVYDPEADAAANADEM
                     AEA"
     assembly_gap    1625..1740
                     /estimated_length=116
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1741..1927
                     /locus_tag="PLV68_19580"
     CDS             <1741..1927
                     /locus_tag="PLV68_19580"
                     /inference="COORDINATES: protein motif:HMM:NF025064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03508.1"
                     /translation="CALLGQLIDADREIVTIVEGVDATAAHTDELRAWLAAEHPGVTV
                     EVHRGGQPLYPYLFGVE"
BASE COUNT          308 a          629 c          644 g          275 t
ORIGIN      
        1 gcttggtgcg gcggtgcgag tgggacacgg tcatacccca ggacggaccc ttgccacaga
       61 tttcacagac agctgccatc ggagatcgct ctttccagga cacggccccg ggcgcgtggg
      121 gctcaatcag aagacggggc aggctaccag cgcggaggcg aagtccctca cagtggatct
      181 ggcaccgggg tctgggacga ggccgatcgc tacgatgctc gggtgcctgc cgccgaccct
      241 ctcgacagcc tgacgccgcg acacggtggt cgcgttcagc acggcgatgc aggctcacgc
      301 ggctgacatc aaccgcctca acgtgtaccc cgtgcccgac ggcgacaccg gtaccaacat
      361 gtcccgcacg ctcgcggcgg tggtggccga actcgacgct gccgaactcg atcgtgccgg
      421 actgagtgct gctggtgcag acatggcgag tatctgcacc gcgatcagcc acgggtcgct
      481 gatgggggcg cgcggcaaca gcggcgtgat cctgtcgcag atcctgcgcg ggctggcgtc
      541 ggtgctgcgt gatctcgacg agcatggtgc cgacgcagcg cagttcgcga ccgcgttgac
      601 caccgcatcg gcggcggcct atcaggcggt gctcaagccg atcgaaggca cgatcctcac
      661 ggtggtccgt gaggcatcgg aagcggcgac ggctgccgcg gccgatggag cgtcgttggc
      721 cgacaccatt cgcgccgccc gccaccaggg tggcgacgca ctggcgcgca cccccgacct
      781 gttgccggtg ctgcgcgacg ccggggtggt cgatgccggg ggagcgggat tcctcctgct
      841 gctcgacgcc gccctgcacg tggtcgccgg tgacgacatg cccgagccca gcgatctggc
      901 cgatggcgcg gccgacgtac cgcaaccgtt cacgccgcgc tccggccatg acggcgagct
      961 tgacgtcagc gagcagcgct acgaggtgat gtacttcctc gacctggccg acgagcagat
     1021 cggggcgttc aagcaggcgt ggggtgacct cggtgactcg atcgtggtcg tcgggggcga
     1081 cgggttgtgg aactgccatg tccacaccaa cgacatcggc gcggccctcg aagcagcgct
     1141 cgatctcggc ggtcgaccgc gtcagattcg cgtgaccgac ctgttcgcgg aagtggctga
     1201 ccagcacgcg gcccgcgaag cggcgatcca ccagccagcg cacaccagct tgatcggtgc
     1261 gagccacgtc caccccggca tcaaccaacc tgcggtgacg tgcgcgatcg tggcggtcag
     1321 cagcggccag gggctgaccg agctgttcgc caacctgggc gtgcaaggcg tgatctccgg
     1381 aggccagacg ctcaacccgt ccacggccga actgctcgac gcggtcgagg ccgtcaacag
     1441 cgaccaggtc gtcgtgctgc ccaacaacaa gaacatcatc cccgtcgccg agcaactcga
     1501 tgcgttgacg cacaagacgg tcgtggtcgt ccccaccgtg agcatgcccg aggcgttggc
     1561 ggcgctgatc gtgtacgacc ccgaagccga tgccgccgcc aacgcggacg aaatggcaga
     1621 ggccnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 gtgcgcgctg ctgggccaac tgatcgacgc cgaccgcgag atcgtcacca tcgtcgaagg
     1801 tgtcgacgcg accgcggcgc acaccgacga actgcgggcg tggctcgcgg ccgagcaccc
     1861 cggtgtgacg gtcgaggtgc accgcggcgg tcagccgttg tatccgtatc tgttcggcgt
     1921 ggagtgacgc gaccggtgga tgcgcccgtc gatgcccggt ccgcccaggt ag
//
LOCUS       DAORNF010000663         1972 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_42502_length_1972_cov_1.306208, whole genome shotgun sequence.
ACCESSION   DAORNF010000663 DAORNF010000000
VERSION     DAORNF010000663.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1972
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42502_length_1972_cov_1.306208"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>241
                     /locus_tag="PLV68_19585"
     CDS             <1..>241
                     /locus_tag="PLV68_19585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03509.1"
                     /translation="VDGGQIWTIESVDDTGAVARLDAATRRFRLGAAVVTAGRQRGPL
                     RVAEGAAAPSPASIVVYDGLRQFREQARKGKPAYVV"
     assembly_gap    242..448
                     /estimated_length=207
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<449..1404)
                     /locus_tag="PLV68_19590"
     CDS             complement(<449..1404)
                     /locus_tag="PLV68_19590"
                     /inference="COORDINATES: protein motif:HMM:NF024801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HRE03510.1"
                     /translation="MSESSSPTTRTTGAVQRGTTDKGTFDRDTNYITTRITADGRDGY
                     PVEPGRYRLVVAKACPWANRTIIVRRLLGLEPVLSMGQCGPTHDVNSWTFDLDPGGVD
                     PVLGLERLQQAYFARYPAYPRGITVPAIVEIATGQVVTNDFPQITIDFSLEWTEFHRP
                     GAPALYPPDLRDEIDTVNKRVYTEVNNGVYRCGFATDQAAYDLAYDRLFTALDWLSER
                     LSTQRYLVGDTITEALARFDPVYHGHFKCNRDKLSEMPVLWAYARDLFQTPGFGDTID
                     FDQIKAHYYVVHTAINPTQVIPKGPDLSGWNDPHGREVLGGRP"
     gene            1484..>1972
                     /locus_tag="PLV68_19595"
     CDS             1484..>1972
                     /locus_tag="PLV68_19595"
                     /inference="COORDINATES: protein motif:HMM:NF012432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HRE03511.1"
                     /translation="MSTDLPIKRPVQALWGPAQALWGPAQALWGKETQHALDNFPVSG
                     RPLPIEIVHALADIKAEAAVVNLMLGAEAVTGRAVSDDDRRRAVAIEAAARAVAMGEY
                     DDQFLIDVFQTGSGTSTNMNVNEVVAALATAACGLAVHPNDDVNRSQSSNDTFPTATT
                     IAV"
BASE COUNT          272 a          546 c          661 g          286 t
ORIGIN      
        1 ggtcgacggt gggcagatct ggacgatcga gtcggtcgac gacactggtg ccgtggcgcg
       61 cctcgacgcg gcgacgcgcc ggttccgttt gggcgctgcc gtcgtcaccg ccgggcgcca
      121 gcgcggtccc ttgcgcgtgg ccgaaggcgc cgccgcgccg tccccggcca gcatcgtggt
      181 ctacgacggg ctgcggcagt tccgtgaaca ggcccgcaag ggcaagccgg cctacgtcgt
      241 gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnngg tcgaccgccg agcacctccc ggccgtgtgg
      481 gtcgttccat ccggaaaggt cgggaccctt gggtatgacc tgggtcgggt tgatggccgt
      541 gtgcaccacg tagtagtgcg ccttgatctg gtcgaagtcg atcgtgtcgc cgaagcccgg
      601 ggtctggaac aggtcgcgcg cgtaggccca cagcaccggc atctcgctga gcttgtcgcg
      661 gttgcacttg aagtggccgt ggtagacggg atcgaaccgg gcgagggctt cggtgatcgt
      721 gtcgccgacc aggtagcgct gcgtgctgag gcgctcgctc agccagtcga gcgcggtgaa
      781 gaggcggtcg taggccaggt cgtacgcggc ctgatcggtg gcgaacccgc agcgatagac
      841 cccgttgttg acctcggtgt agacccgctt gttgaccgtg tcgatctcgt cgcgcagatc
      901 gggcggatag agcgcgggag caccgggccg gtggaactcg gtccactcca ggctgaagtc
      961 gatggtgatc tgggggaagt cgttggtgac cacctggccg gtggcgatct cgacgatcgc
     1021 gggcacggtg atgcctcgtg ggtaggcggg gtagcgggca aagtacgcct gctgcaggcg
     1081 ctcgaggccg agcactggat cgacaccgcc ggggtcgagg tcgaaggtcc agctgttgac
     1141 gtcgtgcgtc gggccgcact ggcccatcga cagcaccggc tcgagaccga gcagacggcg
     1201 gacgatgatg gtgcggttgg cccacggaca tgccttggcg accaccagcc ggtagcggcc
     1261 gggctcgacc gggtagccgt cacgcccgtc ggcggtgatg cgggtggtga tgtagttggt
     1321 atctcgatcg aacgtgccct tgtcggtggt gccgcgctgc accgcgccgg tggtgcgggt
     1381 ggtgggggag gacgactcgc tcatgcgagt gaccgtagcc cggggcgacg atgtgagcca
     1441 caggcctggc tgcgggtgcc gaatcggggt atgactaggc acgatgagca ccgatctgcc
     1501 aatcaagcga ccagttcagg cactgtgggg cccggctcag gcactgtggg gcccagctca
     1561 ggcactgtgg ggcaaggaga cgcagcacgc actggacaac tttcccgtat cggggcgccc
     1621 gctgccgatc gagatcgtgc acgccttggc cgacatcaag gccgaggccg cagtcgtgaa
     1681 cctgatgctc ggcgccgaag ccgtcaccgg gcgcgcggtc agcgacgacg atcgtcgccg
     1741 cgccgtggcg atcgaggcgg ccgcccgcgc cgtggcgatg ggggagtacg acgaccagtt
     1801 cttgatcgac gtgttccaga cgggctcggg gacctcgacc aacatgaacg tcaacgaggt
     1861 cgtggccgcc ctggccacag ccgcgtgcgg tctggcggtg caccccaacg acgacgtcaa
     1921 ccgctcgcag tcgtcgaacg acacgttccc caccgcgacg acgatcgcgg tg
//
LOCUS       DAORNF010000664         1970 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_42551_length_1970_cov_1.546214, whole genome shotgun sequence.
ACCESSION   DAORNF010000664 DAORNF010000000
VERSION     DAORNF010000664.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1970
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42551_length_1970_cov_1.546214"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            48..545
                     /locus_tag="PLV68_19600"
     CDS             48..545
                     /locus_tag="PLV68_19600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03512.1"
                     /translation="MPESAAPDHANADHANPDHANPDHAKPDHGGEAACLRHVVDDIG
                     ADTSGALTIADLTAFAGTGFDASGPSGAVWSLPHGGDLDANLVTLHAGDSIGEHVNGE
                     VDVLMVVIAGHGTVTVAGSTRALQPRGLAFIPKGLARAVHATEALTYLSIHRRRDGGL
                     HITRR"
     assembly_gap    758..806
                     /estimated_length=49
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<807..1026)
                     /locus_tag="PLV68_19605"
     CDS             complement(<807..1026)
                     /locus_tag="PLV68_19605"
                     /inference="COORDINATES: protein motif:HMM:NF020873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein"
                     /protein_id="HRE03513.1"
                     /translation="MPRSLPLGVRYVRTTDVFSPATAPAGLSRAHRVASGVWARLVVS
                     VGHVEFVFEDTPGVRVTVTAGETVAIPPL"
     gene            complement(1088..>1598)
                     /locus_tag="PLV68_19610"
     CDS             complement(1088..>1598)
                     /locus_tag="PLV68_19610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRE03514.1"
                     /translation="VDEAGDDLAPGPDVIGQLCVRGAVVIKGYLNRPEATADAIRDGW
                     FNTGDIARIDEDGFVYIVDRAKDMVLRGGENIYCSEVETAIYHHPAVAEAAVFGIPDE
                     RLGESVAAAIVLHPGTALSEEELQAFLVERIAKPKIPTTVWFLAEQLPRNANGKFVKR
                     ELRKSLIGE"
     assembly_gap    1599..1738
                     /estimated_length=140
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1739..>1970)
                     /locus_tag="PLV68_19615"
     CDS             complement(<1739..>1970)
                     /locus_tag="PLV68_19615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRE03515.1"
                     /translation="ACNCLLHPATLTGARIVFTHKWDPGRALELIEREGVTNFSGVPT
                     MSRELLAHPDWATRDTSSLRGMGGGGAALQPDL"
BASE COUNT          278 a          647 c          580 g          276 t
ORIGIN      
        1 ctgggaccgc ttcgcgtcgg tcaccgcgac cgccttcgag cgcgtccatg cctgagtccg
       61 ctgcccccga ccatgccaac gcggaccatg ccaacccgga ccatgccaat cccgatcatg
      121 ccaaacccga tcatggtggc gaggcggcct gcctgcgaca cgtcgtcgat gacatcggcg
      181 ccgataccag cggcgccctg acgatcgccg acctgaccgc gttcgccggc actgggttcg
      241 acgccagcgg gccgtcgggc gcggtgtgga gcctgcccca cggcggcgac ctcgacgcca
      301 acctggtcac gctgcacgca ggcgactcga tcggtgagca cgtcaacggc gaggtcgacg
      361 tgttgatggt ggtgatcgcc gggcacggca cggtcaccgt cgccggttcg actcgcgctc
      421 tgcaaccgcg cggcctggcc ttcatcccca aggggttggc ccgcgcggtc cacgccaccg
      481 aggcgctgac ctacctcagc atccaccgcc gtcgcgacgg cggcctccac atcacccgcc
      541 gctagcccga cggcaccagg caacggtgtt cattccaccc cctgagccgg ggccaatcga
      601 acacacatcg gcgcccaccg cgagacggtg ttcattccac cccctgagcc ggggccaatc
      661 gaacacagtc gggctggaca cgggtcagtc gctggcgagg ccggtgctct cactgccttc
      721 gggcatcgcg tcggcgccgt cgaccacccg atagaacnnn nnnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn nnnnnngtag cggcgggatc gccacggtct caccggccgt
      841 gaccgtgacc cgcacgccgg gcgtgtcctc gaacacgaac tcgacatggc cgaccgacac
      901 gacgagccga gcccacacgc cactggcgac gcggtgggcg cggctcaacc ccgcgggtgc
      961 ggtcgcgggg ctgaacacgt cggtcgtgcg gacgtagcga acccctagcg gtaacgacct
     1021 cggcatgctc acgacctcac gctcctctcg actggtgtca ttcgactggg ttctgggacc
     1081 ggccctgcta ttcgccgatc aggctcttgc gcagctcgcg cttgacgaat ttgccgttgg
     1141 cgttgcgcgg tagctgctcg gccaagaacc acaccgtggt ggggatcttc ggcttggcga
     1201 tccgctcgac caggaaggcc tgcaactcct cctcgctcag cgccgtgccg gggtggagca
     1261 cgatcgccgc ggcgacgctt tcaccaaggc gctcgtcggg gatgccgaac acggccgctt
     1321 cggccacggc gggatggtgg tagatcgcgg tctccacctc gctgcagtag atgttctcgc
     1381 cgccgcgcaa caccatgtcc ttggcccggt cgacgatgta gacgaacccg tcttcgtcga
     1441 tgcgggcgat gtcaccggtg ttgaaccagc cgtcacggat ggcatccgcg gtggcctctg
     1501 gacggttgag gtagcccttg atgacgaccg cgccgcgcac gcagagctgc ccgatcacgt
     1561 cgggacccgg cgcgagatcg tcacccgcct cgtcgaccnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnag
     1741 gtcgggctgc agggccgcgc caccgccacc catgccgcgc agcgagctgg tgtcgcgcgt
     1801 ggcccaatcg ggatgcgcga gaagctcgcg gctcatcgtc ggcacgccgg agaagttggt
     1861 gaccccctcg cgctcgatca gctccagcgc tcgacccggg tcccacttgt gggtgaacac
     1921 gatccgcgct ccggtcaagg tggccgggtg caataggcag ttgcaggcgg
//
LOCUS       DAORNF010000665         1954 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_42966_length_1954_cov_2.030016, whole genome shotgun sequence.
ACCESSION   DAORNF010000665 DAORNF010000000
VERSION     DAORNF010000665.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1954
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42966_length_1954_cov_2.030016"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="PLV68_19620"
     CDS             complement(<1..250)
                     /locus_tag="PLV68_19620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03516.1"
                     /translation="MSAQTPGHGADIARRQVPVKPANSVRLALSFLRVSAMNDMQYRA
                     NLVLQILQSLLSLGAGLVGLAIVFSRTRELAGWSRAELL"
     gene            complement(247..1080)
                     /locus_tag="PLV68_19625"
     CDS             complement(247..1080)
                     /locus_tag="PLV68_19625"
                     /inference="COORDINATES: protein motif:HMM:NF017946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HRE03517.1"
                     /translation="MAEIALSEGRLRSMVAFYAAAARTSIQVQFQYRAANYFYMIGMV
                     VEPAIYLVVWRTIANANGGSVDGYTAGTFAAYYIVWTLVRNMNIVFTPFGWEARIREG
                     ELSGQLLRPLHPLHYDLAFFGGWKVVVIVMWLPIAAVLSLAFRPTLSPSWQQCVVFFF
                     ALWGAYLIRSMLLWVLGMVTFWTTRVAALYEAYFMAELLLSGRLVPMDLMPGWVQDVS
                     WFLPFRWTFGYPITALVGPITSRELYLGLAAQAAWVVVGALLVRMVWRRGIRRFSAVG
                     G"
     gene            complement(1073..>1954)
                     /locus_tag="PLV68_19630"
     CDS             complement(1073..>1954)
                     /locus_tag="PLV68_19630"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRE03518.1"
                     /translation="AGKTTTLKMLSGLLQPSGGVATVAGYVPGRREAALLGRITLVMG
                     NRNQLQWDLPAIDSFELNRAIYRIPRPDFVAMRDELIDLLDIGGLVNKPVRNLSLGER
                     MKAEIAGSLLHRPDVLFLDEPTIGLDVTMQKRIRSFVAEYNRRHGATVLLTSHYMADV
                     QALCKRVVVIHHGRVLYDGDLAELAARFAATKTLVVRLEPDGGGSPPDLSVYGEVVGD
                     EEGAVSLRVERSRVPATTARLLAEQPVLDLTIEEPPIDDVIERVFATESADNEPGESE
                     PAEREPGENEPAETGDG"
BASE COUNT          361 a          699 c          609 g          285 t
ORIGIN      
        1 cgagcaactc ggcgcggctc cacccggcca gttcccgggt gcggctgaac acgatcgcca
       61 gcccgaccag accggccccg agagacagca gcgactggag gatctgcagc accaggttgg
      121 cccggtactg catgtcgttc atcgcgctga cgcgcaggaa cgacagagcg aggcgaacgc
      181 tgttggcggg cttgaccggc acctggcgcc gtgcgatgtc tgcaccgtgg cccggcgtct
      241 gcgcgctcat ccgcccaccg ccgaaaagcg ccggatgccg cgtcgccaca ccatccgcac
      301 caacagtgca ccgaccacca cccacgccgc ctgcgcggcg aggccgaggt acagctcacg
      361 cgaggtgatc ggcccgacca gcgcggtgat ggggtaaccg aacgtccacc ggaacggcag
      421 gaaccagctg acgtcctgca cccaacccgg catcaggtcc atcggcacca gccgccccga
      481 cagcagcagt tcggccatga agtacgcctc gtacaaggcg gccacgcggg tggtccagaa
      541 cgtcaccatg cccagcaccc acaacagcat cgagcggatc aggtacgcac cccacagcgc
      601 gaagaagaac acgacgcact gttgccaact cggcgagagc gtgggccgga acgccagcga
      661 cagcaccgct gcgatcggca gccacatcac gatcacgacc accttccagc cgccgaagaa
      721 ggcaaggtcg tagtgcaacg gatggagcgg acgcagcaac tggccggaca gttcgccctc
      781 gcggatgcgg gcctcccacc cgaacggcgt gaagacgatg ttcatgttcc gcaccagggt
      841 ccacacgatg tagtacgcgg caaacgtgcc cgccgtgtag ccgtcgaccg agccgccatt
      901 ggcgtttgcg atcgtgcgcc acaccaccag atagatcgcc ggttcgacca ccatgccgat
      961 catgtagaag tagttggcgg cgcggtactg gaactgcacc tggatcgacg tgcgggccgc
     1021 ggccgcgtag aacgcgacca tcgatcgcag ccgcccttcg ctgagcgcga tctcagccat
     1081 cgcccgtctc ggctggctcg ttctcgcctg gctcgcgctc ggctggctcg ctctcacctg
     1141 gctcgttgtc ggccgactcg gtggcgaaga cccgctcgat cacgtcgtcg atcggcggtt
     1201 cttcgatggt caggtccagc acgggctgtt cggccaacag ccgggcggtg gtcgcgggca
     1261 cccgcgatcg ttccactcgc aacgacaccg caccttcctc gtcaccgacc acctcgccgt
     1321 acacgctgag gtcgggtggc gacccaccac cgtcgggctc cagccgaacc accaacgtct
     1381 tggtcgcggc gaagcgggca gccagttcgg ccaggtcgcc gtcgtacagc acccgcccgt
     1441 ggtggatgac caccacccgc ttgcacagcg cctgtacgtc ggccatgtaa tgactggtca
     1501 gcaacacggt cgccccgtgg cggcggttgt actcggcgac gaacgagcgg atgcgcttct
     1561 gcatcgtgac atccaggccg atcgtcggct cgtccaagaa caacacgtcg ggccgatgca
     1621 gcagcgaacc agcgatctcg gccttcatcc gctcgcccag cgacaggttg cgcacgggct
     1681 tgttgaccag tccgccgatg tcgagcaggt cgatcaactc gtcgcgcatc gccacgaagt
     1741 cgggacgggg gatgcggtag atcgcccggt tgagctcgaa gctgtcgatc gcaggcaggt
     1801 cccactgcag ttggttgcgg ttacccatca ccagcgtgat ccgcccgagg agcgccgcct
     1861 cgcggcgacc cgggacgtaa ccggccaccg tggccacacc accgctcggt tgcagcaggc
     1921 ccgacaacat cttcagcgtc gtggtcttgc cggc
//
LOCUS       DAORNF010000666         1949 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_43101_length_1949_cov_1.694826, whole genome shotgun sequence.
ACCESSION   DAORNF010000666 DAORNF010000000
VERSION     DAORNF010000666.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43101_length_1949_cov_1.694826"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>309)
                     /locus_tag="PLV68_19635"
     CDS             complement(<1..>309)
                     /locus_tag="PLV68_19635"
                     /inference="COORDINATES: protein motif:HMM:NF025873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HRE03519.1"
                     /translation="EDAQTLFGFLRRDERTTFQVLIATHGVGPSLAMAILGTHTPSAL
                     VDIVAATDAASLTLVPGVGKKTAERLMVELKNRLNLPILDPIGAATSGGGSSALADVR
                     "
     assembly_gap    310..419
                     /estimated_length=110
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(468..>674)
                     /locus_tag="PLV68_19640"
     CDS             complement(468..>674)
                     /locus_tag="PLV68_19640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HRE03520.1"
                     /translation="GWGAADKAQVQKMVQARLGLTSVPKPADAADAAALALCHLAMSP
                     VRKRTNAAVLSHLQHAATNAGAGQ"
     assembly_gap    675..684
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<685..1011)
                     /locus_tag="PLV68_19645"
     CDS             complement(<685..1011)
                     /locus_tag="PLV68_19645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HRE03521.1"
                     /translation="MVLGIDPGLTRCGYAAIQATGPNSASAISLGVLRTPSTDPLPQR
                     LAELRAELAALIHEFRPHAVAIEHVFFQANVRTAMSVGQASGLALAEAWSSGCEVAQY
                     TPNQVKS"
     assembly_gap    1045..1104
                     /estimated_length=60
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1105..1913)
                     /locus_tag="PLV68_19650"
     CDS             complement(<1105..1913)
                     /locus_tag="PLV68_19650"
                     /inference="COORDINATES: protein motif:HMM:NF016430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HRE03522.1"
                     /translation="MADAEDVVQVVAERWLRADHESIVNPAAWLTTVTSRVGLDRLRA
                     RRRDRADYVGPWLPDPVVAYDDQPATAELSDSLTTAFLVLLEKLSPTERLVLLLADVF
                     QQPFSAVAAVVGKSEAATRQLAVRARRRVASSADVRPATTPEQERLATEFVTAIVLGD
                     QQRASALLADDVVLTSDGGPSRHAARRPVVGRDRVARFLTNVTKRQIEVYGARSRVQP
                     GWVNGAPGFVVMTDDQPDTVFAFDTATTATAGAAVITAVYVMLNPTKLIGAA"
BASE COUNT          268 a          638 c          602 g          261 t
ORIGIN      
        1 cacgcacgtc ggccagggca gacgacccac caccactcgt tgcggcgccg atcgggtcga
       61 ggatgggcag gttgagccgg ttcttcagtt cgaccatcaa tcgctcggcg gtcttcttgc
      121 cgactccggg caccagcgtc aacgaggctg cgtcggtcgc ggcgacgatg tcgacgagcg
      181 cgctcggggt gtgcgtaccg aggatggcca tcgccagcga gggaccgacg ccgtgggtgg
      241 cgatcaacac ctggaacgtg gtccgctcgt cgcggcgcaa gaacccgaac agcgtctgcg
      301 cgtcttcacn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc
      421 gctcgagcac cgagccgcgc aacgagccga tcaccgtcgt gttccagtca ctggccagct
      481 cccgcgtttg ttgcggcgtg ctgcaagtgg gacagcaccg cggcattggt gcgcttgcgc
      541 accggcgaca tggccagatg gcacagcgcc agcgccgcgg catcggccgc gtcggccggc
      601 ttgggcacgc tggtcaggcc gaggcgtgcc tgcaccatct tctgcacctg ggccttgtcg
      661 gccgcccccc atccnnnnnn nnnngctctt gacctggttg ggcgtgtact gggcgacctc
      721 gcacccgctg gaccatgcct cggccagagc caggccactg gcctggccga cgctcatcgc
      781 ggtgcggacg ttggcctgga agaagacgtg ctcgatcgcc acggcgtgcg ggcggaactc
      841 gtggatcagt gcagccagtt cggcgcgcag ttcggccagg cgctgcggca gcgggtcggt
      901 ggacggggtg cgcaacacgc ccagactgat cgccgatgcg ctgttgggcc cggtggcctg
      961 gatggccgca tacccgcatc gcgtcaggcc ggggtcgatg cccagcacca cgattggctc
     1021 cgcagccgac ctcgtcaaga acacnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnngccgcc ccgatcaact tggtcgggtt gagcatcacg
     1141 tagaccgcgg tgatcacggc tgcgcccgcg gtcgcggtcg tcgcggtgtc gaacgcgaac
     1201 accgtgtcgg gctggtcgtc ggtcatcacg acgaagcccg gtgcaccgtt gacccagccc
     1261 ggctggacgc gcgaccgcgc gccgtagacc tcgatctggc gtttggtgac gttggtcaag
     1321 aaccgtgcca ctcggtcacg tccgacgacc ggccgcctgg ccgcgtgacg cgacgggccg
     1381 ccatcggagg tcagcacgac gtcgtcggcc agcaacgcgc tggcccgctg ctggtcgccg
     1441 agcacgatcg ccgtgacgaa ctcggtcgcc aaccgctcct gttccggcgt ggtcgccggg
     1501 cgcacgtcgg cgctgctcgc cacccgccgc cgggcgcgta ccgccagttg tcgcgtcgcc
     1561 gcttccgact tgcccaccac cgccgcgacc gccgagaagg gctgctggaa cacgtcggcc
     1621 agcagcagca ccaaccgctc ggtgggcgac agcttctcca gcaacaccaa gaacgccgtg
     1681 gtcaacgagt cgctcaactc ggccgtcgcg ggttgatcgt cgtacgcgac caccgggtcg
     1741 ggcagccacg gcccgacgta gtcggcccgg tcgcgacggc gggcacggag gcgatcgagg
     1801 ccgacccggc tggtcaccgt cgtcagccac gccgcaggat tgacgatcga ctcgtggtcg
     1861 gcccgcagcc agcgctcggc gacgacctgc accacgtcct cggcatctgc caccgacccc
     1921 agcaaccggt acgacaaccc caccaggcg
//
LOCUS       DAORNF010000667         1949 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_43104_length_1949_cov_1.671595, whole genome shotgun sequence.
ACCESSION   DAORNF010000667 DAORNF010000000
VERSION     DAORNF010000667.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43104_length_1949_cov_1.671595"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="PLV68_19655"
     CDS             <1..271
                     /locus_tag="PLV68_19655"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IclR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HRE03523.1"
                     /translation="TDHTITDSPRLLAELAESAERGWTTNDNEHWPGVRALAVPIEDG
                     SGNVTAALGVQGPSDRLSDERLPEVVAALQTSAHRLSLSLPITTF"
     gene            457..>663
                     /locus_tag="PLV68_19660"
     CDS             457..>663
                     /locus_tag="PLV68_19660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03524.1"
                     /translation="MIDVTLHAATDRDVADLIAFWEVAGENSSRPTDSTAAVAALVAR
                     DADALIIARSDNTIVGTVIAGWDGW"
     assembly_gap    664..744
                     /estimated_length=81
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(992..1082)
                     /locus_tag="PLV68_19665"
     tRNA            complement(992..1082)
                     /locus_tag="PLV68_19665"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1046..1048),aa:Ser,seq:cga)
     gene            1228..1647
                     /locus_tag="PLV68_19670"
     CDS             1228..1647
                     /locus_tag="PLV68_19670"
                     /inference="COORDINATES: protein motif:HMM:NF015232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HRE03525.1"
                     /translation="MAELSLIQTPSVADLLHERLDPAALLNGLVRSVAGLIGAALATP
                     DGRSVAHSDHLQHDPSRAAMIAATMGLATQLIGVVGGRELEEVTVRCETGLVVVYAVG
                     TVGVLTVLARPATNLHHVHTEVRARIGQLRGALGGNR"
     gene            1649..>1949
                     /locus_tag="PLV68_19675"
     CDS             1649..>1949
                     /locus_tag="PLV68_19675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03526.1"
                     /translation="MADYTFKILVTGPFAAGKTSLIQSVSQTPVVTTEVATTGDESAV
                     KANTTVAMDFGTFTLSADADGLGEFSSDGYDDASYDAIDDDGEFGSDDTITLLLFG"
BASE COUNT          327 a          672 c          595 g          274 t
ORIGIN      
        1 caccgaccac accatcaccg acagcccgcg tctgctggcc gaactggccg agtcggccga
       61 gcgcggctgg accaccaacg acaacgaaca ctggcccggc gtgcgcgccc tggccgtgcc
      121 gatcgaagac ggctcgggca acgtcaccgc cgcgctcggc gtgcagggcc ccagcgaccg
      181 cctttccgac gagcgccttc ccgaggtggt cgcggccctc cagaccagcg cccaccgcct
      241 cagcctcagc ctccccatca ccaccttctg agccacccgc gcctgcgatc ccctcgctgg
      301 gaacggacgc caccctggcc cagttgagtc gctcctggct ccagccctgg ccccaagacg
      361 gcagcgccgg aactaccgag acatggaagg accgacactt gcggcgcgac gaaaccgact
      421 gggcagtgag gtcacggggc gagcaagctc tggggcatga tcgacgtgac gctgcatgcc
      481 gcgacagatc gtgacgtcgc ggacctgatc gcgttctggg aggtggcggg cgagaactcg
      541 tctcgtccga cggactcgac ggcagcggtg gccgcgttgg tcgcccgtga cgccgacgcg
      601 ctgatcatcg cgcgcagcga caacacgatt gtcggcaccg tgatcgccgg atgggacggc
      661 tggnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnnaggcac gacttgcagc gctcggcgca acccggttcg
      781 atgcgatggt gttcgacgac aacgacctcg gccaacacgc ctggcgcgcc cgcggctaca
      841 ccgcccaacc cgactggtca cgctgggtac gtccgacgtc accctgatcg acccgtgatc
      901 gactcgtatg tcgactcgct tgtcgacccg taacagcacg acgcccgaac cgctggggtt
      961 cgggcgttgc ggatgtcgca cgacatcaca ttggcggaag gggtgggatt tgaacccacg
     1021 gagacgcaga acgcctcaac ggttttcgag accggcccat tcgtccactc tggcaccctt
     1081 ccgtcgggag aggctagcgg tcgacagcgc gactaccgac cccgataccg accccgttac
     1141 cgacgcggac tgacgaccgc gcgaatcggc cctgcggcac cgcaccgaac gctcaagatt
     1201 ccggcaagtc cgccgatagc tgtgttcgtg gcggaactct ccctgatcca gacgccctcg
     1261 gtggcggacc tgctgcacga acggctcgac ccggccgcgt tgttgaacgg tctcgtccgt
     1321 tcggtcgccg ggttgatcgg cgccgcgctg gcgacccccg acggacgcag cgtcgcccac
     1381 agcgaccacc tccagcacga cccgagccgg gcagcgatga tcgcagcgac gatgggtctg
     1441 gcaacgcagc tgatcggtgt ggtcggcgga cgtgagctgg aagaggtgac cgtgcgctgc
     1501 gagaccggat tggtggtcgt ctacgccgtc ggcaccgtcg gtgtcctgac cgtgctggcc
     1561 cgcccggcca ccaacctgca ccacgtgcac accgaggtgc gggcgcgcat cggtcagctg
     1621 cgcggcgcgc tcggagggaa ccgctgacat ggccgactac accttcaaaa tcctggtcac
     1681 cggaccgttc gcggcgggca agacctcact gatccaatcg gtgtcacaga cgcccgtcgt
     1741 gaccaccgag gtcgccacca ccggcgacga atcggcggtg aaggccaaca cgaccgtcgc
     1801 gatggacttc ggcacgttca ccctctcggc cgacgccgac ggtctcggcg agttcagttc
     1861 cgacggatac gacgacgcct cctacgacgc catcgacgac gacggcgagt tcggcagtga
     1921 tgacaccatc accttgctgc tgttcggca
//
LOCUS       DAORNF010000668         1949 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_43107_length_1949_cov_1.518479, whole genome shotgun sequence.
ACCESSION   DAORNF010000668 DAORNF010000000
VERSION     DAORNF010000668.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43107_length_1949_cov_1.518479"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>300)
                     /locus_tag="PLV68_19680"
     CDS             complement(<1..>300)
                     /locus_tag="PLV68_19680"
                     /inference="COORDINATES: protein motif:HMM:NF022188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HRE03527.1"
                     /translation="RPGPLVGHCSGATTLAPLVPHEAFSLHPLQTITGSTTSLVGASA
                     AVAGSTARSLAVARDLAERLGMRAITIGDKDRVAYHAAACVAANFLITLEDAAERL"
     assembly_gap    301..348
                     /estimated_length=48
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<349..608)
                     /locus_tag="PLV68_19685"
     CDS             complement(<349..608)
                     /locus_tag="PLV68_19685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03528.1"
                     /translation="MVDQHAASDITGQADENVADPVIAVVGAGRVGQALIAALDGAHG
                     PFPRGFDGTIDPATTGPRLAEPGVAGRTGVSSFGPRLADIVIL"
     gene            655..>1384
                     /locus_tag="PLV68_19690"
     CDS             655..>1384
                     /locus_tag="PLV68_19690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03529.1"
                     /translation="MNPNSTKAHIAALSLLDRAFIATPDDEIKTLLVTLPADHISAVK
                     RIAGVHPGEGFDHITGVEPDAADPDEADAAADDVAAADDAVAAASATAPVDADVSQVG
                     DEIVGAIRVAARKGRLNGDLQRLGVVLSDACLAECVTQLGDKADLPSKDDLVAVMPGL
                     VERHGKGVVQIMLASTVVGEAPASVTIIEMLKSDELVKLPPVDPKPIAPLLPLKENDA
                     ERESLRAARKERKAAEQAAAKARRD"
     assembly_gap    1385..1645
                     /estimated_length=261
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1646..1873)
                     /locus_tag="PLV68_19695"
     CDS             complement(<1646..1873)
                     /locus_tag="PLV68_19695"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HRE03530.1"
                     /translation="METLTNSIVIAAPAAVVWNLTIDVANWPSVTPTMTSVERLDDGP
                     FGIGSKARVKQPMQRPTTWTVTEFDPERRFAW"
BASE COUNT          272 a          576 c          560 g          232 t
ORIGIN      
        1 agacgctcgg cggcatcctc caacgtgatc aggaagttgg cggccacgca cgcggcggcg
       61 tggtaggcga cgcgatcctt gtcgccgatc gtgatcgcgc gcatgccaag ccgctcggcg
      121 agatcgcggg cgaccgcgag cgatcgcgcg gtactgccgg caacggccgc cgacgcccca
      181 accagcgacg tcgtagaccc ggtgatggtc tgcagcgggt gcaggctgaa cgcctcgtgc
      241 ggcacgagcg gtgccagcgt ggtcgcaccc gaacagtggc cgaccagcgg tcctgggcgg
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnag gatcacgatg
      361 tcggccagcc gcgggccaaa actgctcacg ccagtacgac cggccacgcc aggttcggcc
      421 agccggggcc cagtcgtggc ggggtcgatg gtgccgtcga agccgcgggg aaaggggccg
      481 tgggccccgt cgagcgcagc gatcagggct tgaccgactc ggccggcacc gaccacggcg
      541 atcacggggt cggcaacgtt ctcgtcggcc tggccggtga tgtccgaagc tgcatgctga
      601 tcgaccacgg gcgcagtgta cgaacgtcgc aaaccgctca taacgtgttg gcccgtgaat
      661 ccgaactcga ccaaggccca cattgcggcc ctctcgctgc tcgaccgcgc gttcatcgcc
      721 acgccggacg acgagatcaa gacactgctg gtgaccctcc cggccgacca catcagcgcc
      781 gtcaagcgca tcgcgggggt ccaccccggc gaggggttcg atcacatcac gggtgtcgag
      841 ccagacgccg ccgacccgga cgaggccgat gctgcagccg acgacgtagc ggcagccgac
      901 gacgccgttg ccgcggccag tgcgacggcc cccgtcgatg ccgacgtctc ccaggtgggc
      961 gacgagatcg tcggcgccat ccgcgtcgcc gcccgcaagg gccgcctcaa cggcgacctg
     1021 cagcgcctcg gcgtggtgct cagcgacgcc tgcttggccg agtgcgtcac gcagctcggc
     1081 gacaaggctg acctgccgtc caaggatgat cttgtcgcgg tgatgcccgg cctggtcgag
     1141 cgtcacggca agggtgtcgt gcagatcatg ctcgcctcga ccgtcgtcgg tgaggcaccg
     1201 gcgtcggtca ccatcatcga gatgctcaag agcgacgaac tggtcaagct gccgcccgtc
     1261 gatccgaagc cgatagcgcc gttgctgccg ctcaaggaga acgacgccga gcgcgagtca
     1321 ttgcgcgccg cgcgcaagga gcgcaaggcc gccgaacaag ccgcggccaa agctcgtcgc
     1381 gatcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnccatg cgaagcgacg ctcagggtcg aactcggtca
     1681 ccgtccacgt cgtcgggcgt tgcatcggtt gcttgacgcg ggccttgctg ccgatgccga
     1741 acgggccgtc gtcgaggcgt tccacgctgg tcatcgtcgg tgtcaccgac ggccagttgg
     1801 cgacgtcgat ggtcaggttc cacaccaccg ctgccggtgc ggcgatcacg atcgagttcg
     1861 tcaaggtctc cacacccggc gactgtacgc cacgtcacac caacgtcggc ccgccggcgt
     1921 cacactggcc cggcgtcacc ctggcccgg
//
LOCUS       DAORNF010000669         1946 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_43208_length_1946_cov_1.223691, whole genome shotgun sequence.
ACCESSION   DAORNF010000669 DAORNF010000000
VERSION     DAORNF010000669.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1946)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43208_length_1946_cov_1.223691"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    329..391
                     /estimated_length=63
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<392..>630)
                     /locus_tag="PLV68_19700"
     CDS             complement(<392..>630)
                     /locus_tag="PLV68_19700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03531.1"
                     /translation="AVAEAALGRVNAALAADVEIAPLLDGAPAIRGVTNITDDRYAVR
                     VAVATTPTNRETVRRAWRRMLLVAFAAGELIEPQP"
     assembly_gap    631..779
                     /estimated_length=149
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<780..1264)
                     /locus_tag="PLV68_19705"
     CDS             complement(<780..1264)
                     /locus_tag="PLV68_19705"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HRE03532.1"
                     /translation="MGAIVVFSSVLAASSRPGWVRRLDELHLLTPLRMVLVIAAAIAV
                     TAFVRRMAGRWLDRMFQRAGPADQPRALARHETLSSALRAAVIGVVWAVAVITVISEA
                     GVNIGAFVATATVIGGAIAFGAQTLIRDVIGGFFVLADDQFGVGDEVDLGQATGVVER
                     VT"
     gene            1325..>1686
                     /locus_tag="PLV68_19710"
     CDS             1325..>1686
                     /locus_tag="PLV68_19710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03533.1"
                     /translation="MGDEDATTPPLAPPLSRRHGHRPAHFVILGVNILIALVCFGAAG
                     GLLFGQSVVANQRKTAPLNTTPVTVPRDQPGASIPGGTGDPTTTDEPGTESPTIGQPG
                     ADPDEPFPQAEPAAKNFL"
     assembly_gap    1687..1712
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1713..>1946
                     /locus_tag="PLV68_19715"
     CDS             <1713..>1946
                     /locus_tag="PLV68_19715"
                     /inference="COORDINATES: protein motif:HMM:NF015755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HRE03534.1"
                     /translation="GEGGEASIGDRTSLGERSDTIMIMRVNPETSQAAILSFPRDLYV
                     DLAGTNRKGKINWAYKVDDPQNLIDTIQNNFYVP"
BASE COUNT          293 a          654 c          524 g          237 t
ORIGIN      
        1 cgccgcgttg cgggccgaca tgcacgcccg cggcgagagc accgagtcgt tcgactgggc
       61 caccctgctt gacgaactgc tcggcgggcc gcccgaatct tgacgatcgt ggcgttgggt
      121 gacgcggcga ccacggtgag gagaatcttt gcgcctcggt tgccctccct tggcgatttc
      181 ctcacacgcg ttcaggcagg ctgagcccac aacatgaagc agtgatccgc cacgttgccc
      241 catctgtccc gctgtcccgc ccgacggggt gccgaacgga tcactggttg tggctcacac
      301 cgccaccgtc gaccacactt cccgccgcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nccggttggg gctcgatcag ttcgcccgcg
      421 gcgaaggcca ccagcagcat tcgccgccat gcccgccgga cggtctcgcg gttggtgggc
      481 gtggtcgcga ccgcgacccg cacggcgtag cggtcgtcgg tgatgttggt gacgccgcgg
      541 atcgccggtg cgccgtcgag cagcggagcg atctccacat cggccgccaa tgcggcattg
      601 acccggccga gcgccgcttc ggccaccgcg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng
      781 tcacccgctc gaccacgccg gtggcttggc cgaggtcgac ctcgtcgccg actccgaact
      841 ggtcatcggc cagcacgaaa aacccgccga tcacgtcgcg gatcagggtc tgggccccga
      901 acgcgatcgc accaccgatc accgtcgcgg tcgcgacgaa tgcgccgatg ttgacgccag
      961 cctcgctgat caccgtgatc accgccaccg cccagaccac gccgatcact gcggcccgca
     1021 gcgccgacga cagggtttcg tgacgggcca gggcacgcgg ctggtcggcg ggtccggccc
     1081 gctggaacat ccgatcgagc cagcgtccgg ccatgcgccg cacgaatgct gtgactgcga
     1141 tcgcggccgc gatcaccagc accatccgca gcggcgtcaa caggtgcagt tcgtccaacc
     1201 gccgcaccca gccgggccgc gacgaagccg cgagcacgga actgaacacg acgatggcac
     1261 ccatcggggt caacgtagcg cccgcggcca cctgctggcg cggcgcaggt agagtcgcaa
     1321 cttcatgggc gacgaggacg cgacaactcc accgttagct cccccgctca gccgccgaca
     1381 cggccaccgc ccggcccatt tcgtgatcct cggcgtcaac atcctgatcg cgctggtgtg
     1441 cttcggcgcc gcggggggcc tgctcttcgg ccagagcgtc gtcgccaacc aacgcaagac
     1501 ggcgccgttg aacaccaccc ctgtcaccgt cccccgcgat cagccgggcg cctccatccc
     1561 cggcggcacc ggcgacccga ccaccaccga cgagcctggc accgagtcgc ccaccatcgg
     1621 ccaaccgggt gccgaccctg acgagccgtt cccccaagcc gaaccggccg ccaagaactt
     1681 cctgatnnnn nnnnnnnnnn nnnnnnnnnn nncggcgaag gcggcgaggc ctcgatcggc
     1741 gaccgcacca gcctcggcga gcgcagcgac acgatcatga tcatgcgggt caaccccgag
     1801 accagccagg ccgcgatcct gtcctttccc cgcgacctgt acgtcgacct cgctggcacc
     1861 aaccgcaagg gcaagatcaa ctgggcctac aaggtcgacg acccacagaa tctgatcgac
     1921 acgatccaga acaacttcta cgtccc
//
LOCUS       DAORNF010000670         1929 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_43628_length_1929_cov_1.735326, whole genome shotgun sequence.
ACCESSION   DAORNF010000670 DAORNF010000000
VERSION     DAORNF010000670.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43628_length_1929_cov_1.735326"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>344
                     /locus_tag="PLV68_19720"
     CDS             <1..>344
                     /locus_tag="PLV68_19720"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HRE03535.1"
                     /translation="VWATLIALVHADRGPVLGLVSAPAMGRRWWAASGHGAFVNGRPI
                     RVSGIDALDDAQVCVTFNRGWDLLGLTPRLVALQQQAYRARGFGDFWQHMLVAEGAVE
                     VAVDAVGVQPYD"
     assembly_gap    345..354
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    601..724
                     /estimated_length=124
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            753..>990
                     /locus_tag="PLV68_19725"
     CDS             753..>990
                     /locus_tag="PLV68_19725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03536.1"
                     /translation="MIRRLVALAILVAVIVGLVAGVRSLLGDSSEAKGDTPSSLTSTP
                     QTPDTSGSGTSGSGTSGSDTPSSTDAASVTNPPAV"
     assembly_gap    991..1120
                     /estimated_length=130
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1121..1915
                     /locus_tag="PLV68_19730"
     CDS             <1121..1915
                     /locus_tag="PLV68_19730"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRE03537.1"
                     /translation="GLVRNDAFDWPAQLATLVPSVDPDISVAFFGGNDSQGFSTPLIQ
                     KIDSPEWRAEYAARVDQFIGLLGGPNRTVIWIGIPTHPGKANAGLAVQTEVVRERLAA
                     HPEVHYVDLWSLFAGINGGFSKLSRDPFTGEYIEVRTKVGEDYFHLNTRGTKILASLV
                     TNLIADDLVARGATQAADMKTALYPQDQPGLYEIVDNDNLTVIAKRVGTTIDAIVAVN
                     GWADENHVIFAKQKIKLPAPPLVQPATAVASNEPDPSTTTPPTSTG"
BASE COUNT          275 a          638 c          500 g          252 t
ORIGIN      
        1 ggtgtgggcc acgttgatcg cgctcgtcca cgccgaccgc gggccggtgt tgggcctggt
       61 gtcggctccc gcgatggggc gacggtggtg ggccgcgagc ggccacggcg cgttcgtcaa
      121 cgggcgcccg atccgcgtgt cgggcatcga cgcgctcgac gacgcccagg tgtgcgtcac
      181 gttcaaccgc gggtgggacc tgctcggcct cacgccacgg ctggtcgcgc tgcagcagca
      241 ggcctaccgg gcacggggct tcggcgactt ctggcagcac atgctggtgg ccgagggtgc
      301 ggtcgaagtc gccgtcgacg cggtcggcgt gcagccctac gactnnnnnn nnnncgtggt
      361 gcgcgaagcg ggcgggcagt tcaccgatcg gctcggccaa cccacctacg aacacgactc
      421 cgcgatcagc tccaacggct tgttgcacga caccgtgctc gacacgatca accgccgatg
      481 agcaacggtc aaccccgctc cggtcaaccc cgctcaggcc aaccgcgctc ggcccagtca
      541 cgcgcgggcc agtcaggccg gtcgcgctcg gcgtcgtcgc gatcgcaacc atcacagtcg
      601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      721 nnnntcgacc gcggccgacc gcgcgtacat tcatgatccg ccgcctcgtc gccttggcaa
      781 tcctggtcgc ggtcatcgtc gggttggtgg ccggcgtgcg ctcgctgctg ggcgactcat
      841 ccgaggccaa aggcgacacc ccgtcgtcgc tgacctccac cccgcagact cctgacacat
      901 cgggctctgg cacgtcagga tctggcacgt ccggttccga caccccctcg tccacggacg
      961 cggcatcggt caccaacccg ccagcggtgc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ggcctcgtcc gcaacgacgc
     1141 cttcgattgg cccgcgcaac tcgccacgct ggtgccgagc gtcgaccccg acatctccgt
     1201 tgccttcttc ggcggcaacg actcccaagg tttctcgacc ccgctcatcc agaagatcga
     1261 ctcgcccgaa tggcgcgccg agtacgcagc acgtgtcgat cagttcatcg gcctccttgg
     1321 cggccccaac cgcaccgtga tctggatcgg catcccgacc caccccggca aggccaacgc
     1381 gggtctcgcc gtccagaccg aggtcgttcg tgaacgcctc gccgcgcacc ctgaggtgca
     1441 ctacgtcgat ctgtggtcgc tgttcgcagg catcaacggt ggcttctcga aactgtcccg
     1501 cgacccgttc acgggtgagt acatcgaggt ccgcaccaag gtcggggagg actacttcca
     1561 tctcaacacc cgcggcacca agatcctcgc ctcgctggtc accaacctga tcgccgacga
     1621 cctggtcgcc cgcggcgcga cccaggcggc cgacatgaag accgcgctct acccgcagga
     1681 ccagccgggc ctgtacgaga tcgtcgacaa cgacaacctc accgtcatcg ccaagcgggt
     1741 cggcaccacg atcgacgcga tcgtcgccgt caacggctgg gccgatgaga accacgtcat
     1801 cttcgccaaa cagaagatca agctgcccgc tcccccgctg gtgcaaccgg ccacagccgt
     1861 cgccagcaac gaacccgacc cgagcaccac cacacccccc acatccaccg gctgactctc
     1921 ccccaccag
//
LOCUS       DAORNF010000671         1928 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_43673_length_1928_cov_1.320876, whole genome shotgun sequence.
ACCESSION   DAORNF010000671 DAORNF010000000
VERSION     DAORNF010000671.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1928
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43673_length_1928_cov_1.320876"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(110..886)
                     /locus_tag="PLV68_19735"
     CDS             complement(110..886)
                     /locus_tag="PLV68_19735"
                     /inference="COORDINATES: protein motif:HMM:NF017946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HRE03538.1"
                     /translation="MLRVWRALWRVRVLHELHYRANLAMNLVQVAIDLAIGAVAIKLV
                     FANIDDLHGWDEDGLLVVLGIYTMIDAFVRAVVLPNMFALVEDVRDGNFDGVLVMPAD
                     AQVFVTARELSVWDLPGLALGAVVVVVGAVRQGGIGPAEVGLFVVTFPFGLAIIYGFF
                     LAVTSLVFRLIDLRDLLFRLFQGSSSGRWPLGIYPGWLRVVLTVVVPMAVAVTVPASA
                     LTGRLGAAQVGLAVLAAVVVLTVSRWLFRRGLRSYAGASA"
     assembly_gap    1081..1304
                     /estimated_length=224
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1305..1738)
                     /locus_tag="PLV68_19740"
     CDS             complement(<1305..1738)
                     /locus_tag="PLV68_19740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03539.1"
                     /translation="MIAGGAPGWLRRHAEFYRVSTAVELKTALQYRWPTLLGMVALMC
                     EPVVYLAVWTSVARANGGSVGGLTLGQVSAYYVVWTVARNLVAGVSPEVWQARFREGL
                     LAEMLLRPIHPVHNDLAGGLGGNVPRMVMTVPITVVLVLVFRP"
     gene            complement(1735..>1928)
                     /locus_tag="PLV68_19745"
     CDS             complement(1735..>1928)
                     /locus_tag="PLV68_19745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03540.1"
                     /translation="PADLASFGTVVEATGNTVVLRVERADTAATTARLMANFDVADLT
                     VADPPIEDLIARLFTAGTP"
BASE COUNT          327 a          644 c          540 g          193 t
ORIGIN      
        1 cgctgccgac cccacccgaa ctcgacgccc ccggcctctg gctagcccgc ctcgacatcg
       61 ccccaacccc cgactaaccg gcgggctgga cccggctgga ccgggctggt caggcagagg
      121 cgccggcgta cgagcgcagg ccgcggcgga acagccagcg gctcacagtc agcacgacca
      181 ccgcggcgag cacggcgaga ccgacctgcg cagcacccag gcggcccgtc agcgccgacg
      241 ccggcaccgt gaccgcgacc gccatcggca cgaccacggt caacaccacc cgcaaccatc
      301 cggggtagat gccgagtggc cagcgtcccg acgaggaacc ctggaacagg cggaacaaca
      361 ggtcgcgcag atcgatcagg cggaacacca gcgaggtgac ggccaggaag aacccgtaga
      421 tgatcgcaag tccgaacggg aacgtcacca cgaacagccc gacctcggcc gggccgatgc
      481 caccttggcg caccgcgccg accaccacca ccaccgcgcc gagcgccaga cccggcagat
      541 cccacaccga cagctcccgg gccgtgacga acacctgggc gtcggcgggc atgacgagca
      601 ccccgtcgaa gttgccgtcg cgcacgtctt cgacgagggc gaacatgttg ggcagcacca
      661 ccgcccgtac gaaggcatcg atcatcgtgt agatgccgag caccaccagc aggccgtcct
      721 cgtcccagcc gtgcaggtcg tcgatgttgg cgaacacgag cttgatcgcc accgcaccga
      781 tcgccaggtc gatcgcaacc tgtaccaggt tcatcgccag gttggcccgg tagtgcagtt
      841 cgtgcaggac gcgcacccgc cacagggcgc gccacacgcg cagcacgtgg ccagctcccg
      901 atggcggcat cagatgccca ccgagtcgaa gcggcgcacg gccaacttcc acagtgccga
      961 cagcgcacac ccggcaacga ccagccagcc gagttgaacg gcgagtccga cggcgacctc
     1021 gcggccgctc atcgaggtgg tgacgacctc gatcgggaac ccgaacgtgg atcggaacgg
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnggccgg aacaccagga
     1321 cgagcacgac cgtgatcggc acggtcatca ccatccgcgg cacgttgccc cccaagccac
     1381 cggccaggtc gttgtgcacc ggatggatgg ggcgcaacag catctctgcc agcagccctt
     1441 cgcggaaccg cgcctgccag acctcgggcg acacgcccgc gaccaggttg cgggccacgg
     1501 tccacacgac gtagtaggcg ctgacctgtc ccaacgtcag ccccccgacc gaaccgccgt
     1561 tggcccgggc gacagaggtc cacaccgcca ggtacacgac cggctcgcac atcagcgcga
     1621 ccatgccgag cagcgttggc cagcggtact gcagcgccgt cttgagctcg accgccgtgc
     1681 tcaccctgta gaactcggcg tggcggcgca accaccccgg cgcacccccg gcgatcatgg
     1741 tgttccagcg gtgaacagtc gggcgatcaa gtcctcgatc ggcgggtcgg ccaccgtcag
     1801 gtcggcgacg tcgaagttgg ccatcagccg cgccgtcgtc gccgcggtgt cggcccgttc
     1861 cacccgcagc accaccgtgt tgccggtggc ttcgaccacg gtgccgaacg aagcgaggtc
     1921 ggcaggtg
//
LOCUS       DAORNF010000672         1927 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_43699_length_1927_cov_1.282051, whole genome shotgun sequence.
ACCESSION   DAORNF010000672 DAORNF010000000
VERSION     DAORNF010000672.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1927
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43699_length_1927_cov_1.282051"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(66..>241)
                     /locus_tag="PLV68_19750"
     CDS             complement(66..>241)
                     /locus_tag="PLV68_19750"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03541.1"
                     /translation="FAWGAMPVGAAVGGLIARATNQRVPYLAAAACVVVGVVIMLGTV
                     TTRTLAEAGGKNR"
     assembly_gap    242..255
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<256..>549)
                     /locus_tag="PLV68_19755"
     CDS             complement(<256..>549)
                     /locus_tag="PLV68_19755"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03542.1"
                     /translation="NRWYGVMLGIMAAGGIVAGLVGERIIARLGKITTLYAAAIVWIV
                     TMTVIGLVPVLVVVVAALTLGVFTVTLWNIGAVSLRQQLVPAAVFGRVNSVYR"
     assembly_gap    550..663
                     /estimated_length=114
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<664..1321)
                     /locus_tag="PLV68_19760"
     CDS             complement(<664..1321)
                     /locus_tag="PLV68_19760"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRE03543.1"
                     /translation="MNPPESTLPPQLGHRRLGRAYWRLWTASTVSNLGDGMFLVAMPL
                     LAARTTDDAFAVALVTAANTLPWLLLSLHAGAIVDRHDRRRLLVIGDSFRGALLVLLT
                     VIVAFDVMQIWMLWLLAFCLGIAEVVFDNAAQAILPALVEPDQLEKANGRRYSAELLA
                     NVFLGTPLGGALFAVAVWLPFGVDAVGYLAAAVLVFGLRGNFANDAPTGPPQSLWDDV
                     K"
     gene            1441..1659
                     /locus_tag="PLV68_19765"
     CDS             1441..1659
                     /locus_tag="PLV68_19765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03544.1"
                     /translation="MAVTKWSVSVEETLAARVESRVGDRGLSGFVARAVEHELERDLL
                     GDYLDELDAEFGPVAKDLIEAVDDLWQS"
     gene            complement(1656..>1927)
                     /locus_tag="PLV68_19770"
     CDS             complement(1656..>1927)
                     /locus_tag="PLV68_19770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03545.1"
                     /translation="ASLNRGEQVARNARNPCTGRDDLCDATSEHRIDCFVGTPPSVQL
                     DEYGSRNANRCVVCVGASHCCPHPFVAHGIGMWASERRGGLAVKD"
BASE COUNT          340 a          638 c          568 g          253 t
ORIGIN      
        1 cctcacccgt tgatcccgtt gatcccgttg atccggttgg tcgcgttgaa cggacgaccc
       61 accggtcagc ggttcttgcc gcctgcctcg gccagggtgc gggtggtgac ggtgcccaac
      121 atgatcacca cgccgacgac cacgcacgca gccgcggcga ggtagggaac ccgctggttg
      181 gtggcccggg cgatcagacc gcccaccgcc gcgcccactg gcatcgcacc ccaggcgaac
      241 cnnnnnnnnn nnnnncagcg gtacacgctg ttgacgcgcc cgaacaccgc ggcgggaacc
      301 aattgctggc gcagcgacac ggcgccgatg ttccacagcg tcaccgtgaa cacccccagc
      361 gtcagggccg cgaccaccac gaccagcacc ggcaccaggc cgatcaccgt catcgtcacg
      421 atccacacga tggccgcggc gtagagcgtg gtgatcttgc cgaggcgggc gatgatccgc
      481 tcgccgacca acccggcgac gatgccaccg gcggccatga tgcccagcat caccccgtac
      541 cagcggttgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      661 nnnacttgac gtcgtcccac aacgattgcg gcggcccggt gggcgcgtcg ttggcgaagt
      721 tgccgcgaag gccgaacacc aacaccgccg ccgccagata gccgaccgcg tcgactccga
      781 agggcagcca caccgccacc gcgaacagcg caccgccgag aggcgtgccg agaaagacgt
      841 tggccaacag ttcggccgag taacgccgcc cgttggcctt ttccagctgg tccggctcga
      901 ccagcgcggg caggatcgcc tgggccgcgt tgtcgaacac cacctcggcg atgcccaagc
      961 agaacgccag cagccacagc atccagatct gcatcacgtc gaacgccacg atcacggtca
     1021 gcagcaccag caacgcgccc cggaacgaat cgccgatcac gagcaggcgg cgccggtcgt
     1081 ggcggtcgac gatcgccccc gcatgcagcg agagcagcaa ccagggcagc gtgttggccg
     1141 ccgtcaccag cgccacggcg aacgcgtcgt cggtagtgcg cgctgccaac aacggcatcg
     1201 ccaccaggaa catgccgtcg ccgaggttgg aaacggtgct cgccgtccac agccgccaat
     1261 aggcacgacc aagccgccgg tggccgagct gaggcggcag cgtcgactca ggcggattca
     1321 tggaccacaa gactgccacc cgaatcacgc ttgtccgcga ccggacgcgt gccctccgac
     1381 acgcccacgg tcgcgtcgca gagccctcga cagtgtttcc taccgtggta ggatttgatc
     1441 atggctgtca caaagtggtc cgtcagcgtc gaggagacac tcgctgcgcg cgtcgagagt
     1501 cgggtgggag atcgcggact cagcgggttc gtcgcacgag ccgtcgagca cgaactcgaa
     1561 cgcgatctgc ttggtgacta tctcgatgaa ctcgacgcag agttcgggcc agttgcaaag
     1621 gatctcatcg aagcagtcga tgacctgtgg cagtcctagt ccttgacagc gaggccgcct
     1681 ctacgctcgc tcgcccacat gccgatcccg tgcgccacaa acgggtgcgg gcagcaatgc
     1741 gaagcgccca cgcacaccac gcaccggttc gcattccggc tgccgtactc gtcgagttgt
     1801 accgagggcg gggtgccgac gaagcaatcg atgcgatgct cgctcgtcgc gtcgcacagg
     1861 tcgtcacgac cggtgcacgg attgcgcgca ttgcgggcca cctgctcgcc gcggttgagg
     1921 ctggcag
//
LOCUS       DAORNF010000673         1924 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_43763_length_1924_cov_1.540396, whole genome shotgun sequence.
ACCESSION   DAORNF010000673 DAORNF010000000
VERSION     DAORNF010000673.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1924
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43763_length_1924_cov_1.540396"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>525)
                     /locus_tag="PLV68_19775"
     CDS             complement(<1..>525)
                     /locus_tag="PLV68_19775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03546.1"
                     /translation="FISYGNGVTTTSAYEPLTFRLHTLVSSGGRQSLEYRYDPVGNIL
                     GIDNNADDTLYFDNAAVEPHQSFQYDALYRLTNAWGREKSARGRADWVEPAFGPVPDT
                     SETMQGYEQRYSYDVAGNITEMRHLVSATTDWVRTYTYPSTSNRLSTTTETDGTFSYQ
                     HDVRGNIVFWPHLY"
     assembly_gap    526..567
                     /estimated_length=42
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<568..>1924)
                     /locus_tag="PLV68_19780"
     CDS             complement(<568..>1924)
                     /locus_tag="PLV68_19780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03547.1"
                     /translation="AMAVVGNASEGDTLSSPTAEFEYDTSSVPAWAWGRVRETHGVAV
                     GSTRWIETTEYSDGAGSVVMTKATAAPDGATARWIGSGRVVLNNKGLPIKQYEPFYST
                     TDAFEAEEGFSGVTPLITYDPLGRAIRVDMPNGTLRRIEFSPWEQTTWDENDTVTESE
                     WYDERIGQSPAHEEEDLALDAAEHADTPTTVRLDVLGRPFQTIERLDESTELLTTLTL
                     DAVGNPLGVTDPREVEIQTQSFDMLGRPITTESPDGGDVTALLDVAGQPLFLWKSGDL
                     GIETEVDALRRKVRTWQWDTDAETRVLRERTVFGEGLDDSGTYDPKDDHLRGRLYRIY
                     DTAGMVELSYDFKGNVLTTTRRFFDDDEEDINWQSFDPVAYPFVGDDKDSVAELHNGD
                     GGSNPGAASLLESEEFTVSATFDAMNRVVTRTTPDGAVTAHEYDDGGRLNLVEVDSDT
                     IV"
BASE COUNT          248 a          592 c          687 g          355 t
ORIGIN      
        1 tgtagaggtg cggccagaag acgatgttgc cccgcacgtc gtgctggtag ctgaacgtac
       61 cgtccgtctc cgtcgtggtc gagaggcggt tgctggtgct ggggtaggtg taggtccgca
      121 cccagtcggt cgtggcgctg acgaggtgcc gcatctcggt gatgttgccg gcgacgtcgt
      181 agctataccg ttgctcatac ccctgcatgg tctcgctggt gtcgggcaca ggcccgaagg
      241 cgggctcgac ccagtcggcg cggccgcggg cggacttctc gcggccccag gcgttcgtca
      301 ggcgatacag cgcgtcgtac tgaaagctct ggtggggctc gaccgcggcg ttgtcgaagt
      361 acagcgtgtc gtcggcgttg ttgtcgatgc ccaggatgtt gccgaccggg tcgtagcggt
      421 actccagcga ctgccggccc ccgctgctga ccagggtgtg caggcggaag gtcaagggct
      481 cgtaggcgct cgtggtggtg accccgttgc cgtacgagat gaaggnnnnn nnnnnnnnnn
      541 nnnnnnnnnn nnnnnnnnnn nnnnnnncga cgatggtgtc gctgtcgacc tcgacgaggt
      601 tgaggcgccc gccgtcgtcg tattcgtgcg cagtcaccgc cccgtcgggg gtggtgcgcg
      661 tgacgacgcg gttcatggcg tcgaaggtcg cagagacggt gaactcctcg ctctccagca
      721 ggctcgcggc gccggggttg ctgcccccgt cgccgttgtg cagctcggcc accgagtcct
      781 tgtcgtcccc gacgaacggg tacgcgaccg gatcgaagga ctgccagttg atatcctctt
      841 catcgtcgtc gaagaatcgg cgggtggtgg tcagcacgtt gcccttgaag tcgtagctga
      901 gttccaccat acccgcggtg tcgtagatcc ggtacagccg cccgcggagg tggtcgtcct
      961 tggggtcgta cgtgcccgag tcgtcgagcc cctcgccgaa gacggtgcgc tcgcgcagca
     1021 cccgggtttc ggcgtcggtg tcccactgcc aggtgcggac tttgcggcgc agcgcgtcga
     1081 cctcggtctc gatgcccagg tcgccggact tccagaggaa gaggggctgg cctgcgacat
     1141 cgaggagagc ggtgacgtcg ccgccgtcag gggactcggt ggtgatgggg cggccgagca
     1201 tgtcgaagga ctgggtctgg atctcgacct cgcgggggtc ggtgaccccc aaggggttgc
     1261 ccacggcgtc cagggtcagc gtggtcagca gctcggtgct ctcgtccagg cgctcgatgg
     1321 tctggaaagg acgccccagc acatccaggc gcaccgtggt cggcgtgtcg gcgtgctccg
     1381 ccgcgtccag cgccaggtcc tcttcctcgt gggccgggct ctggccgatg cgctcgtcat
     1441 accactcgct ctcggtgacc gtgtcgttct cgtcccaggt ggtctgctcc cacggcgaga
     1501 actcgatgcg gcgcagcgtg ccgttgggca tgtcgacgcg gatggcgcgg ccgagggggt
     1561 cataggtgat cagcggcgtc acgcccgaga agccttcctc ggcttcgaag gcgtcggtgg
     1621 tggagtagaa gggctcgtac tgcttgatgg gcaggccctt gttgttcagc accacccggc
     1681 cgcttccgat ccaccgcgct gtggccccgt ccggtgcggc ggtggccttc gtcatgacga
     1741 cggacccggc cccgtcgctg tactcagtgg tttcgatcca gcgggtgctg ccgacggcga
     1801 ccccgtgggt ctcgcgcacg cggccccagg cccaggcggg aacgctgctg gtgtcgtact
     1861 cgaactcggc ggtgggcgag gagagcgtgt cgccctcgga ggcgttgccg acgaccgcca
     1921 tcgc
//
LOCUS       DAORNF010000674         1921 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_43848_length_1921_cov_2.019829, whole genome shotgun sequence.
ACCESSION   DAORNF010000674 DAORNF010000000
VERSION     DAORNF010000674.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43848_length_1921_cov_2.019829"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1106
                     /locus_tag="PLV68_19785"
     CDS             <1..1106
                     /locus_tag="PLV68_19785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HRE03548.1"
                     /translation="MRALGLDVQIDAIGNVVATRHGTDPTLAPVMTGSHIDTVRTGGR
                     FDGNLGVLAGLEVIETLIQHDVRTPRGVQVAFFTDEEGARFAPDMLGSLVYVGGLALE
                     EALDARAADDGVRLGDELTRIGYAGPTPCPAGSAPHAFVELHIEQGPVLEDEGTTIGV
                     VTGVQGISWTELTITGTSAHAGTTPMHLRHDPLVAAAQIALDVHQIAAAIGSGQVATV
                     GRFDPFPDLVNVVAQRVTMTVDLRNTDEGLLRRAEAMLAASIERAVAVQRCTVETRSL
                     ARFEPVAFDAGMIERVETTAARLGHSARRMPSGAGHDAQMLARVCPTSMIFVPSVGGL
                     SHNIAEFTEPHHIAAGADVLLHVILAAAGTTEM"
     assembly_gap    1175..1184
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1249..>1921
                     /locus_tag="PLV68_19790"
     CDS             1249..>1921
                     /locus_tag="PLV68_19790"
                     /inference="COORDINATES: protein motif:HMM:NF012242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HRE03549.1"
                     /translation="MPSWILAIDFGTSYTVAASKVDDRPPEVIEIGGERRVPSVIMVG
                     QDGEVVVGRVADDLSATHPESTLRAMKNRLGDQAPVVLDGRPHQVVALVSALLAQVYA
                     EASRQMGEPPTEVRLTHPATWNRPRLNRLLEAAAKAGLPNPALVPEPVAAALSFASEV
                     GVPAGAHVVVYDLGGGTFDTAVVTSNGSGFNIVGRPSGDQNIGGELFDEIIVNRYQAF
                     VIDILS"
BASE COUNT          284 a          669 c          652 g          306 t
ORIGIN      
        1 ggatgcgcgc cctcggcctc gacgtgcaga tcgacgcgat cggcaacgtc gtcgccaccc
       61 gccacggcac cgatcccacc ctcgctcccg tgatgaccgg ctcgcacatc gacaccgtgc
      121 gcacgggcgg gcgcttcgac ggcaacctcg gcgtgctcgc cgggttggag gtgatcgaga
      181 ccctgatcca acacgacgtg cgcacgccgc gcggtgtgca ggtggcgttc ttcaccgacg
      241 aggaaggtgc gcgcttcgcg cccgacatgc tcggcagcct ggtctacgtc ggcggcctcg
      301 cgttggagga ggccctcgat gcgcgggcag ccgacgacgg cgtccgcctc ggcgacgagc
      361 tgacccgcat cggctatgcc gggccgacgc cgtgccccgc tgggtccgcc ccgcacgcct
      421 tcgtcgaact gcatatcgag caagggccgg tgctggagga cgagggcacc acgatcggtg
      481 tcgtcaccgg ggtgcagggc atctcgtgga ccgagttgac catcacgggt acatcggccc
      541 atgcgggcac cacgccgatg catctgcgcc acgacccgtt ggtggctgcg gcacagatcg
      601 cgctcgacgt gcatcagatc gcggccgcga tcggatcggg gcaggtcgcc accgtcggcc
      661 gcttcgaccc gttccccgat ctcgtcaatg tcgtcgccca acgggtgacg atgaccgtcg
      721 atctgcgcaa caccgacgag gggttgctgc gccgggccga agcgatgctg gccgcgtcga
      781 tcgagcgcgc ggtcgcggtg caacgctgca cggtcgagac ccgttcgctg gcccgcttcg
      841 agccggtcgc gttcgacgcg ggaatgatcg agcgagtcga gacgaccgcc gctcgactgg
      901 ggcactcggc gcggcggatg ccgtccggtg cgggtcacga cgcgcagatg ctggcgcggg
      961 tgtgtcccac ctcgatgatc ttcgtgccct cggtcggcgg gctgtcgcac aacatcgccg
     1021 agttcaccga gccgcaccac atcgccgcgg gcgcggacgt gctgctgcac gtgatcctcg
     1081 ccgcggcggg caccacagag atgtagctgg ggccggggca ttcggcggag gagtcgccga
     1141 accgcacgcg gagccgctgc gctaatgtcg ctgcnnnnnn nnnnaacctg cgtcgttgca
     1201 tgcaacccgc gtcaacgtat ctcgcgtcag agaaggagtc tggttccctt gccttcgtgg
     1261 attctcgcca tcgatttcgg aaccagctat accgtcgctg cctccaaggt tgatgatcgc
     1321 ccacccgagg tgatcgagat cggcggtgag cgccgcgtgc cgtcggtgat catggtcggc
     1381 caggacggcg aggttgtggt cggccgggtc gccgacgacc tcagcgcgac ccaccccgaa
     1441 tcgaccctgc gggcgatgaa gaaccgtctc ggtgaccagg ccccggtggt gctcgacggc
     1501 cgtccgcacc aggtggtcgc gctggtgtcg gcgctgctgg cccaggtcta cgccgaggcg
     1561 tcgcgccaga tgggcgaacc gccgaccgag gtccgcctca cccatcccgc cacctggaac
     1621 cggccccggc tcaaccgcct gctcgaagcc gcggccaagg cgggcttgcc caacccggcc
     1681 ctggtgcccg agccggttgc cgcggcgctc agcttcgcca gcgaggtcgg cgttcccgca
     1741 ggtgcccacg tcgtggtcta cgacctgggt ggcggcacgt tcgacaccgc cgtggtcacg
     1801 tccaacggca gcgggttcaa catcgtcggg cgtccgtcgg gcgaccagaa catcggtggt
     1861 gaactgttcg acgagatcat cgtcaaccga tatcaagcct ttgttattga catcctctcc
     1921 g
//
LOCUS       DAORNF010000675         1920 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_43894_length_1920_cov_1.277212, whole genome shotgun sequence.
ACCESSION   DAORNF010000675 DAORNF010000000
VERSION     DAORNF010000675.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1920
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43894_length_1920_cov_1.277212"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>239)
                     /gene="glnA"
                     /locus_tag="PLV68_19795"
     CDS             complement(<1..>239)
                     /gene="glnA"
                     /locus_tag="PLV68_19795"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797717.1"
                     /note="forms a homododecamer; forms glutamine from ammonia
                     and glutamate with the conversion of ATP to ADP and
                     phosphate; also functions in the assimilation of ammonia;
                     highly regulated protein controlled by the
                     addition/removal of adenylyl groups by adenylyltransferase
                     from specific tyrosine residues; addition of adenylyl
                     groups results in inactivation of the enzyme; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HRE03550.1"
                     /translation="YKRLIPGFEAPVLLAYSARNRSASCRIPVVSNPKGKRVEVRFPD
                     PLSNPYLAFSAMLMAGLDGIQNKIHPGDPIDKNLY"
     assembly_gap    240..400
                     /estimated_length=161
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<401..644)
                     /locus_tag="PLV68_19800"
     CDS             complement(<401..644)
                     /locus_tag="PLV68_19800"
                     /inference="COORDINATES: protein motif:HMM:NF012348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HRE03551.1"
                     /translation="MPPIDSAQDMRSEMLAMIEQMGFETEKHHHEVAASQHELGLKFG
                     KLVKCADAMIAYKYCVQMVAHKWGKTATFMPKPVYGD"
     assembly_gap    679..748
                     /estimated_length=70
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<749..>1009)
                     /locus_tag="PLV68_19805"
     CDS             complement(<749..>1009)
                     /locus_tag="PLV68_19805"
                     /inference="COORDINATES: protein motif:HMM:NF012348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase beta-grasp
                     domain-containing protein"
                     /protein_id="HRE03552.1"
                     /translation="GWKAINESDMTLMPDPETVTMDPFTAQPTMVMYCDVIEPSTGQG
                     YTRDPRSIAKRAEAYVKQTGLADTVFMGPEAEFFVFDDVRFDV"
     assembly_gap    1010..1063
                     /estimated_length=54
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1285..1347)
                     /locus_tag="PLV68_19810"
     CDS             complement(1285..1347)
                     /locus_tag="PLV68_19810"
                     /inference="COORDINATES: protein motif:HMM:NF012753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03553.1"
                     /translation="MSTVDDVIRIRTGERGNEAV"
     assembly_gap    1371..1455
                     /estimated_length=85
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1456..1744)
                     /locus_tag="PLV68_19815"
     CDS             complement(<1456..1744)
                     /locus_tag="PLV68_19815"
                     /inference="COORDINATES: protein motif:HMM:NF012753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HRE03554.1"
                     /translation="MRSEGVARNHAALDRWHGPCIFSSRGGRAKVGPRPHHPRRSMKK
                     IEAIIKPFKLDEVKEALNDLGIKGLTVTEAKGFGRQKGHTELYRGAEYVVDF"
BASE COUNT          249 a          479 c          501 g          321 t
ORIGIN      
        1 cgtagaggtt cttgtcgatc gggtcgccgg ggtggatctt gttctggatg ccgtcgagac
       61 ccgccatcag catcgccgag aaggcgaggt aggggttcga cagcggatcc gggaagcgca
      121 cctcgacgcg cttgcccttg gggttcgaca ccaccgggat gcggcacgag gccgagcggt
      181 tgcgcgccga ataggcgagc agcaccggcg cctcgaaacc gggaatgagg cgcttgtagn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tgtcgccata gaccggcttc
      421 ggcatgaacg tcgccgtctt gccccacttg tgcgcgacca tctgcacgca gtatttgtag
      481 gcgatcatcg cgtcggcgca cttcacgagc ttgccgaact tcaggcccag ctcgtgctgc
      541 gaggcggcga cctcgtgatg gtgcttctcg gtctcgaagc ccatctgctc gatcatcgcc
      601 aacatctcgg agcgcatgtc ctgcgcgctg tcgatcggcg gcaccgggaa atagccgccc
      661 ttgaccggcg gacggtggnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnga cgtcgaagcg cacgtcgtcg aacacgaaga
      781 actcggcctc ggggcccatg aacacggtgt cggcgaggcc ggtctgcttg acgtaggcct
      841 cggcgcgctt ggcgatcgag cgcggatcgc gcgtgtagcc ctggccggtc gagggctcga
      901 tcacgtcgca atacatgacc atcgtcggct gcgccgtgaa cgggtccatc gtcaccgtct
      961 cggggtccgg catcagcgtc atgtcggatt cgttgatcgc cttccagccn nnnnnnnnnn
     1021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngcggatt cgtccatcgt
     1081 ccgcggcacc tgcgtgacgt gctgccactt gccgcgcgga tcggtgaacc ggaggtcgac
     1141 gtactggacg tccttgtcct tcatcatctg gatgacgtct ttggccgtgg tcatgggtct
     1201 tgcttcccct tgctgagatt tcggaacggt tgtcgggacg agcggcgccg aatgcgatgc
     1261 gcgaaacggc gcaggcgggt tgcgctacac cgcctcgttg ccgcgctcgc cggtgcggat
     1321 gcggatcacg tcgtccaccg tgctgatgaa gatcttgccg tcgccgatgc nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnggaaa tcgacgacgt attcggcgcc gcggtagagc tcggtgtgcc
     1501 ccttctgccg gccgaagccc ttggcttcgg tgacggtcag gcccttgatg ccgagatcgt
     1561 tgagcgcttc cttcacctcg tcgagcttga acggcttgat gatcgcttcg atctttttca
     1621 ttgatcgcct tggatgatgt gggcgcggcc cgaccttcgc ccggccgccg cggctgctaa
     1681 agatgcacgg accatgccag cgatcgagcg ccgcatgatt ccgcgcaacg ccttcgcttc
     1741 gcaagtcgat tctggtgcat acttaggccg aattctgctc aaaacacggg caacaagtct
     1801 attcttgcgg cgtttttagg ctcggcagcg cgtcagccga gggctttgcg aatgcgcgac
     1861 agggcttcgc gaatattctc ggcactgttt gcataggaaa agcgcagata cccctcgcca
//
LOCUS       DAORNF010000676         1914 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_44034_length_1914_cov_1.549758, whole genome shotgun sequence.
ACCESSION   DAORNF010000676 DAORNF010000000
VERSION     DAORNF010000676.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44034_length_1914_cov_1.549758"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="PLV68_19820"
     CDS             <1..185
                     /locus_tag="PLV68_19820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03555.1"
                     /translation="VKIVSGNLVNKTQTATLVVEQKYQDFEKSTAYAASFLLALAAVL
                     CIVVVATLRPKEPDQP"
     gene            196..1224
                     /locus_tag="PLV68_19825"
     CDS             196..1224
                     /locus_tag="PLV68_19825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter ATP-binding protein"
                     /protein_id="HRE03556.1"
                     /translation="MSIDVRGVTKRFGNFVALDDVSVHIATGRLTALLGPSGGGKSTL
                     LRIIAGLERADVGEVHIAGADATHLSPQRRNVGFVFQHYAAFKHMTVAKNVAFGLEVR
                     KRDKAEIAARVDELLHLVHLEAFADRLPAQLSGGQRQRMALARALAVEPAVLLLDEPF
                     GALDAKVRAELREWLRRLHDEVHVTTVLVTHDQEEALEVADQIVVINDGRIEQVGSPD
                     DLYDAPASRFVMSFLGPVTQLGGALVRPHDIEVLTTPEPASHPATVTRVNRVGFDVRV
                     EFAVADGNGDPTLATMTRTHAATLDLHPGRSVWLRTEPGAVTMGATTADPIGGFAATD
                     ERPLVTAG"
     gene            1295..>1488
                     /locus_tag="PLV68_19830"
     CDS             1295..>1488
                     /locus_tag="PLV68_19830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03557.1"
                     /translation="MRPDGTAPRGTAKSAASRAVWHLILVVAYAGMVAGLVQLYRGMR
                     EVLAIGGSCGSGGPYVYVRE"
     assembly_gap    1489..1655
                     /estimated_length=167
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1656..>1914
                     /locus_tag="PLV68_19835"
     CDS             <1656..>1914
                     /locus_tag="PLV68_19835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03558.1"
                     /translation="GWLNPIDGTKQVGFIICAVVFFAMGGLPLLGAIWVFVRNRLRGG
                     SAATGESPANVISKNLGLGGTAGLGGASGAAAAAAAAAAAQR"
BASE COUNT          264 a          626 c          587 g          270 t
ORIGIN      
        1 cggtcaagat cgtgtccggc aacctcgtca acaagaccca gacggccacg ctggtggtcg
       61 aacagaagta ccaagacttc gagaagtcga ccgcgtacgc ggcgtcgttc ctgctggccc
      121 tcgctgcggt gctgtgcatc gtcgtcgtgg ccaccctgcg ccccaaggag cccgatcaac
      181 catgagcgtc ccgacatgag catcgacgtc cgcggtgtca ccaaacgctt cggcaacttc
      241 gtcgccctcg acgacgtgtc ggtccacatc gccaccggcc gcttgaccgc gctgctcggc
      301 ccgagcggcg gcggcaagtc gacgctgctg cgcatcatcg ccgggctcga acgggccgac
      361 gtgggtgagg tccacatcgc tggtgccgac gcgacacatc tcagcccgca gcgccgcaac
      421 gtcggcttcg tgttccagca ctacgccgcg ttcaagcaca tgacggtcgc caagaacgtc
      481 gccttcggcc tggaggttcg caagcgcgac aaggccgaga tcgccgcccg tgtcgacgaa
      541 ctgctccacc tggtgcactt ggaagcgttc gccgaccgcc tgcccgcgca gctgtccggc
      601 ggccaaaggc agcggatggc gttggcccgc gcgctggccg tcgagccggc cgtgctgttg
      661 ctcgacgaac cgttcggcgc gctcgacgcc aaggtgcggg ccgagctgcg cgagtggctg
      721 cgccggctgc acgacgaggt tcacgtcacc accgtgctgg tcacccacga ccaagaggaa
      781 gcattggagg tcgccgacca gatcgtggtc atcaacgatg gtcgcatcga acaggtcgga
      841 tcgcccgacg acctctacga cgcgccagcg agccggttcg tgatgagctt tctcgggccg
      901 gtgacccaac tcggcggagc gcttgttcgc ccccacgaca tcgaagtgct gaccacgccc
      961 gagcccgcca gccaccccgc gaccgtgact cgggtgaacc gcgtcggctt cgacgtgcgg
     1021 gtcgagttcg ccgtcgcgga cggcaacggt gacccgacgc tggcgacgat gacgcgcacg
     1081 cacgcggcca cgctcgatct gcaccccggc cgcagcgtgt ggctccgcac cgagccgggt
     1141 gccgtcacca tgggcgccac gacggccgac cccatcggcg ggtttgctgc aaccgacgag
     1201 cgcccactcg tcacggcggg ttgatcgcca gcggttgcgc gacggccacc gtgtcgcgcg
     1261 gtcgtcacac cgaggtcgac tacattcaca tcgcatgcgc ccggatggaa ccgccccacg
     1321 gggcaccgcc aagtcggccg cgtcgcgggc ggtgtggcat ctgatactgg tcgtcgccta
     1381 cgcggggatg gtcgctggac tggtccagtt gtaccgcggc atgcgcgagg tactcgctat
     1441 cggcggttcc tgcggctccg gcggaccgta cgtgtacgtc cgcgagtgnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncggct ggctcaaccc catcgacggc
     1681 accaagcagg tcggcttcat catctgcgcc gtcgtgttct tcgcgatggg cggtctgccc
     1741 ctgctcgggg cgatctgggt gttcgtccgc aaccgcctgc gcggtggctc ggcggcgacc
     1801 ggcgagtccc ccgcgaacgt gatctccaag aacctcggcc tgggcggcac cgccggtctc
     1861 ggtggtgcca gcggcgcggc agcggccgcg gcggctgccg ccgcggcgca gcgg
//
LOCUS       DAORNF010000677         1913 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_44058_length_1913_cov_1.922497, whole genome shotgun sequence.
ACCESSION   DAORNF010000677 DAORNF010000000
VERSION     DAORNF010000677.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44058_length_1913_cov_1.922497"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="PLV68_19840"
     CDS             <1..288
                     /locus_tag="PLV68_19840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRE03559.1"
                     /translation="SVNPATGGLGAELAHRVQEGIGSAYGAAADLGASAPTVLRAAQD
                     ALVDGWRLSMWVGVGIAISALVYLAVRGPRPSDLAAEDALDLSEGLVPEPV"
     gene            343..>1086
                     /locus_tag="PLV68_19845"
     CDS             343..>1086
                     /locus_tag="PLV68_19845"
                     /inference="COORDINATES: protein motif:HMM:NF020932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imelysin family protein"
                     /protein_id="HRE03560.1"
                     /translation="MMSKLSRRVLGSVATSALVFGAVACGSDDDKGSGGKATLKDAVA
                     TYASGVYASYSATLASATAMDVAIDAFVAAPSDATLTAAKNAWLAARNDYGSTEAFRF
                     YGGPIDDEEDGPEGLINAWPLDEAYIDYVEGDAEAGIVNNPDDYPTIDADVLTEANEK
                     DGETNISTGWHAIEFLLWGQDLNEDGPGDRPVEDYTTNPNADRRGTYLAVASDLLLEH
                     LQGLVDAWAPDATNYRAEFLALDPNESGRM"
     assembly_gap    1087..1132
                     /estimated_length=46
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1133..1526
                     /locus_tag="PLV68_19850"
     CDS             <1133..1526
                     /locus_tag="PLV68_19850"
                     /inference="COORDINATES: protein motif:HMM:NF020932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="imelysin family protein"
                     /protein_id="HRE03561.1"
                     /translation="ERLAVSLDSGDQEDEHSCFSDNTTNDIIANAVGIQNVLLATYPG
                     GVTGTSFYDVFAAKDKAAADRLKAEVAASVAAAGTIAAPFDQWLREGVADDAPGRQAI
                     LKTMDDLSTQTDSLVAAAKSIGISIAVS"
     gene            1540..>1913
                     /locus_tag="PLV68_19855"
     CDS             1540..>1913
                     /locus_tag="PLV68_19855"
                     /inference="COORDINATES: protein motif:HMM:NF018269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HRE03562.1"
                     /translation="MQARRARRIAFLVCFVAASCGGSDGGPDGGRVLLDPNLGGGTTR
                     VDASRNSFGLPAPTLDNDERRLFEVGDSFFTQNWVIAPASTTARDGLGPLFNARACAS
                     CHVLDGRGIAGDSASIADFIAMV"
BASE COUNT          300 a          657 c          617 g          293 t
ORIGIN      
        1 agcgtgaacc ccgcgacggg cggcctcggc gccgagctcg cccaccgcgt gcaggagggc
       61 atcgggtcgg cctacggcgc cgcggccgac ctcggtgcga gcgcgccgac ggtgctgcgt
      121 gcggctcagg acgcgctggt cgacggctgg cggctgtcga tgtgggtcgg ggtgggtatc
      181 gccatttcgg cgctggtcta cctcgcggtg cgcggcccca ggccgagtga tctggccgcg
      241 gaagacgcgc tcgatctcag cgagggcctc gtccccgaac ccgtgtgaca ttccggaagg
      301 agctttgccg atcggagttc ggcgtggcta acatcacccg ccatgatgtc caaactttcc
      361 aggcgagttc tcggttcggt tgccacgtcg gcgctggtgt tcggtgcggt ggcatgcggt
      421 agcgacgacg acaagggctc cggcggcaag gcgacgttga aggatgccgt cgccacgtac
      481 gcatccggcg tgtacgcctc gtactcggcg accctggcat cggccacggc gatggacgtc
      541 gcgatcgacg cgttcgtcgc cgccccgagc gacgcaacgt tgaccgctgc caagaacgcc
      601 tggctggccg ctcgcaacga ctacggctcg accgaagcct tccgcttcta tggcggtccg
      661 atcgacgacg aagaggacgg tccggagggt ctcatcaacg cctggccgct cgacgaggcc
      721 tacatcgact acgtcgaggg cgacgccgag gcgggcatcg tcaacaaccc cgacgactat
      781 ccgaccatcg acgccgacgt gctcaccgag gccaacgaga aggacggcga gaccaacatc
      841 tcgactggct ggcacgcgat cgagttcttg ctgtggggcc aggacctcaa cgaggacggt
      901 cctggcgacc ggcccgtcga ggactacacc accaacccca acgccgaccg ccgcggcacc
      961 tacctggccg ttgccagtga cctcctgctc gagcacctcc agggtctcgt cgacgcctgg
     1021 gcccccgacg ccaccaacta tcgagccgag ttcctcgccc tcgatcccaa cgagtcgggg
     1081 cgcatgnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncgagcgtc
     1141 tcgccgtttc cctcgattcc ggtgatcagg aggacgagca ctcctgcttc agcgacaaca
     1201 ccaccaacga catcatcgcc aacgccgtcg gtatccagaa cgtgctgctg gccacatatc
     1261 ctggcggcgt gacgggcacc agcttctacg acgtgttcgc cgccaaggac aaggccgctg
     1321 ccgaccggct caaggccgaa gtcgccgcca gcgtcgccgc cgcgggcacg attgcggccc
     1381 cgttcgacca gtggctgcgt gagggcgtgg ccgacgatgc ccccggtcgc caggcgatcc
     1441 tgaagacgat ggacgacctc agcacgcaga ccgactcgtt ggtcgctgct gccaagtcga
     1501 tcggcatcag catcgcggtg tcgtgacaag ttgacccccg tgcaggcacg gcgcgcgcgt
     1561 cggatcgcat tcctggtctg cttcgtcgcc gcatcgtgcg gcgggtccga tggcgggccc
     1621 gacgggggca gggtgctcct cgacccaaac ctcggtggcg gaacgacccg ggtcgatgcg
     1681 tcacgcaact cgttcggctt gccggccccg acgctggaca acgacgaacg gcgcctgttc
     1741 gaggtcggcg acagcttctt cacccagaac tgggtcatcg cccctgcgtc gacgacggct
     1801 cgtgacgggc tcgggccgtt gttcaacgcc cgggcctgcg cgtcgtgcca cgtgctcgac
     1861 gggcgcggca tcgccggcga ctccgccagc atcgccgact tcatcgccat ggt
//
LOCUS       DAORNF010000678         1910 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_44139_length_1910_cov_1.757951, whole genome shotgun sequence.
ACCESSION   DAORNF010000678 DAORNF010000000
VERSION     DAORNF010000678.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1910
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44139_length_1910_cov_1.757951"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="PLV68_19860"
     CDS             <1..408
                     /locus_tag="PLV68_19860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03563.1"
                     /translation="RELGVKFGQGWLFGRPGGANQPGVCVNPAALSGGDAALGSVLPP
                     GGWLFADGQQMPTITTGAVSVPGLFHGECRTTADGYAYLAITVAADPRDPRGDDITGD
                     GRDNWGLHTVDMNLAQDTLIALVGQQIAAYGNR"
     gene            405..1448
                     /locus_tag="PLV68_19865"
     CDS             405..1448
                     /locus_tag="PLV68_19865"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HRE03564.1"
                     /translation="MSTVPTTAAAPGVSPGLPLSVLDLAMVVAGGSSHAALAATTQLA
                     QAADRLGYTRFWVAEHHNMPSVACTSPTVLMAHLAAVTEHIRVGSGGVMLPNHAPLVV
                     AEQFAMLEALHPDRIDIGIGRAPGTDPRTAAALRRSPEGLGAEDFPRELIDLMGLLGD
                     PRRDEGLWTQFRATPVATSSPAVFLLGSSGYSAQVAGYLGLPFGYAHHFDMGGTEQAV
                     ALYRETFTPSPILDEPYLVITANVLAADTDELANWHADPGRLTMLRRRTGRFMPLPSP
                     ADAAAHPDLEMARGLPSNRIIGDAATCVAELRALAAATDANEMMITAVAYDLDVRIHS
                     LELVAAAWGLTAR"
     gene            complement(1435..>1910)
                     /locus_tag="PLV68_19870"
     CDS             complement(1435..>1910)
                     /locus_tag="PLV68_19870"
                     /inference="COORDINATES: protein motif:HMM:NF012506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HRE03565.1"
                     /translation="GAPIRALALLDEIGTPDRIDAVARPLIERGDKIMGFGHAVYRTD
                     DPRSVMLRGIAERLAAATGQKPFVRFATAVEDRVIALLDEWKPGRKLRTNVEFYAGVV
                     MEMCGVPRSMFTPTFASSRVIGWCANVLEQAADNKIIRPSARYVGPPPPQPVSAL"
BASE COUNT          276 a          708 c          646 g          280 t
ORIGIN      
        1 cgcgagctgg gggtgaagtt cggacagggc tggctgttcg gccggccggg cggggcgaac
       61 caaccgggcg tgtgcgtcaa cccggccgcg ctctccggcg gtgacgccgc gctcggcagc
      121 gtgctgccgc ccggtgggtg gctgttcgcc gacggtcagc agatgcccac gatcaccact
      181 ggcgccgtca gcgtgccggg tctgttccat ggcgaatgcc gcaccactgc cgacggctac
      241 gcctacctgg cgatcacggt tgcggccgac ccccgcgacc cccgcggcga cgacatcacc
      301 ggcgatggtc gcgacaactg gggtctgcac accgtcgaca tgaacctcgc ccaggacacc
      361 ctcatcgcgc tggtgggtca gcagatcgcc gcgtacggca accggtgagc accgtgccca
      421 ccaccgctgc ggctcctggc gtgtcacccg gcctgccctt gtcggtgctc gatctggcga
      481 tggtggtcgc cggcggatcg agccatgcgg ccctcgccgc cacgacacag ctggcccagg
      541 ccgcggaccg gctgggctac acccggttct gggtcgcgga gcaccacaac atgcccagtg
      601 tcgcgtgcac ctcgccgacg gtgctgatgg cccacctggc cgcggtcacc gagcacatcc
      661 gcgtcggcag cggcggggtg atgctgccca atcacgctcc gctggtggtg gccgagcagt
      721 tcgcgatgct cgaagcgctg cacccggacc gcatcgacat cggcatcggc cgcgccccgg
      781 gtaccgaccc gcgcacggcg gcggcgttgc ggcgctcgcc cgagggtctg ggtgccgagg
      841 acttcccccg cgagctgatc gacctgatgg gcctgctcgg cgatccccgg cgcgacgaag
      901 ggctgtggac ccagttccgg gcaacgccgg tggccacgtc gtcgcccgct gtgttcctgc
      961 tggggtccag cggctacagc gcgcaagtgg ccggatacct cggtctgccg ttcggctacg
     1021 cccaccactt cgacatgggc ggcaccgaac aggccgtcgc gctgtaccgc gagacgttca
     1081 cgccgtcgcc gatcctcgac gagccctacc tggtgatcac cgccaacgtg ctcgccgccg
     1141 acaccgacga gctggccaac tggcacgccg acccaggccg gttgacgatg ctccgccgcc
     1201 gcaccgggcg cttcatgccc ttgccgtcac ccgccgacgc ggcggcgcat cccgacctgg
     1261 agatggcgcg cgggctgccg agcaaccgca tcatcggtga cgccgccacg tgtgtggccg
     1321 aactccgcgc gctggcggcc gcgaccgacg cgaacgaaat gatgatcacc gcggttgcct
     1381 acgacctcga cgttcgcatc cacagcctgg agctggtcgc cgcggcgtgg ggactcacag
     1441 cgcgctgacc ggctgcgggg gcggtggccc gacgtagcga gcactgggac ggatgatctt
     1501 gttgtcagct gcctgctcca gcacattggc gcaccagccg atcacccggc tcgacgcgaa
     1561 cgtcggggtg aacatcgacc gcggcacgcc gcacatctcc atcaccacac ccgcgtagaa
     1621 ctcgacgttg gtccgcagct tgcggcccgg cttccactcg tcgagcagcg cgatcacccg
     1681 atcctcgact gccgtggcga aacggacgaa cggcttctga ccggtcgcgg cagccagtcg
     1741 ctcagcgatg ccgcgcagca tcaccgagcg cgggtcgtcg gtgcggtaca ccgcgtggcc
     1801 gaaacccatg atcttgtcgc cgcgttcgat cagcggccgg gctacggcgt cgatgcgatc
     1861 gggcgtgccg atctcgtcga gcagcgccag cgcccggatc ggtgccccgc
//
LOCUS       DAORNF010000679         1909 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_44163_length_1909_cov_1.620820, whole genome shotgun sequence.
ACCESSION   DAORNF010000679 DAORNF010000000
VERSION     DAORNF010000679.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44163_length_1909_cov_1.620820"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>217
                     /locus_tag="PLV68_19875"
     CDS             <1..>217
                     /locus_tag="PLV68_19875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03566.1"
                     /translation="RTGRVSATLATGITVVDDEVDVDLSVAGHDRSLVLRVAAAAAHA
                     QYPISRRSLIKLSATAPDDGERWDERTK"
     assembly_gap    218..381
                     /estimated_length=164
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            399..>915
                     /locus_tag="PLV68_19880"
     CDS             399..>915
                     /locus_tag="PLV68_19880"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRE03567.1"
                     /translation="MRTVARPDLLLIGALLHDIGKGYPGDHTEVGMELVGTIVPRMGF
                     DDDDTAIVRSLVEHHLLLSETALRRDLADPRTAENVAAAVGDQTRLELLRALTEADSR
                     ATGPSAWSAWKETLLDELTAAVAAVLAGHEAVADDIDHEVVHASILGRVRTDQTAHTA
                     RVQTDDFELWTV"
     assembly_gap    916..947
                     /estimated_length=32
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <948..1444
                     /locus_tag="PLV68_19885"
     CDS             <948..1444
                     /locus_tag="PLV68_19885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03568.1"
                     /translation="AFAMLGVDVVSADVWTSADGIAVDTFRIPLASMARIEPAKLDKE
                     LRAVVTGSVDIGGRIAQRIRTYSRYHRRAVAAALPRFDVLISNDASATTTMIDVRAPD
                     DIAVLYRLASCLTDLHLDIRSAKVATLGHEVVDVFYVQRRGQVPTAEHDSIRAALTVA
                     LTKT"
     gene            complement(1452..>1909)
                     /locus_tag="PLV68_19890"
     CDS             complement(1452..>1909)
                     /locus_tag="PLV68_19890"
                     /inference="COORDINATES: protein motif:HMM:NF023326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3418 domain-containing protein"
                     /protein_id="HRE03569.1"
                     /translation="ALRSAGRILAVAADTAAKLDRLVTPPVQPSVTDARSHLAHLVRP
                     GFVTAAGIDRLDDVLRYVRGVATRVAKIPEDPARDRRSIAEAVSVEAHAKALFDKVPP
                     SRVTREMVELGWLTEELRVSLFAQTLGTSVPVSSKRIMRELDRLDRELA"
BASE COUNT          259 a          618 c          593 g          243 t
ORIGIN      
        1 gcgcaccggg cgggtgtcgg ccaccctcgc cacgggcatc acggtggtcg acgacgaggt
       61 cgatgtcgac ctttccgttg ccggccacga ccgctcgctg gtgctgcgcg tcgctgccgc
      121 tgcagcccac gcgcagtacc cgatcagccg ccgcagcctc atcaagctgt cggcaacggc
      181 gcccgacgac ggtgagcgct gggacgagcg caccaagnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nccgaagcaa gccgtctcgt gcgcacggtc gcccggcccg
      421 acctgttgct gatcggcgcg ctgctgcacg acatcggcaa gggctacccc ggcgaccaca
      481 ccgaggtcgg gatggaactg gtcggcacga tcgtgccgcg catgggtttc gacgacgacg
      541 acacggcgat cgtccgctcg ctggtcgagc accacctgct gttgtcagag acggcgctgc
      601 gccgcgacct tgccgacccg cgcaccgcgg agaacgtcgc cgccgccgtc ggcgatcaga
      661 cccggctgga actgctgcgg gcgctgaccg aggccgacag tcgtgccacc ggtccatcgg
      721 cctggtcggc gtggaaggag acgttgctcg acgagctgac cgccgcggtc gcggccgtgc
      781 tcgccgggca cgaggccgtc gccgacgaca tcgatcacga agtcgtccac gcctccatcc
      841 tcgggcgggt ccgcaccgat cagaccgccc acaccgcccg ggtgcagacc gacgacttcg
      901 aactgtggac ggtcgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngag cgttcgcgat
      961 gctcggcgtc gacgtggtca gcgccgacgt gtggaccagc gccgacggca tcgcggtcga
     1021 caccttccgt atcccgctgg cgagcatggc ccgcatcgag ccggccaagc tcgacaagga
     1081 actgcgtgcc gtcgtcaccg gatcggtcga catcggtggg cgcatcgccc aacgcatccg
     1141 cacctacagc cgctaccacc gccgtgccgt ggccgctgct ctcccgcggt tcgacgtgct
     1201 gatcagcaac gacgcgtccg cgaccaccac gatgatcgac gtgcgtgccc ccgacgacat
     1261 cgccgtgctg taccgactgg cgtcgtgcct caccgacttg cacctcgaca tccgctcggc
     1321 caaggtggcc accctcggcc acgaggtggt cgacgtgttc tacgtccagc gccgcgggca
     1381 ggtacccacc gcggagcatg actcgatccg ggctgcgctc accgtggcgc tcaccaagac
     1441 ctgacccggc ctcaggccag ctcgcggtcg aggcggtcga gttcgcgcat gatgcgcttg
     1501 gagctgaccg gcaccgacgt gccgagcgtc tgggcgaaca ggctgacgcg cagctcttcg
     1561 gtcagccagc cgagctcgac catctcgcgg gtcacccggc tgggtggcac cttgtcgaac
     1621 aatgccttgg cgtgggcctc gacggacacg gcctcggcga tcgagcgccg gtcgcgggcg
     1681 gggtcctcgg gaatcttggc gacccgggtg gcgacgccgc ggacgtagcg cagcacgtcg
     1741 tcgagacggt cgatgccggc cgctgtgacg aagcccggac gcaccagatg ggcgaggtgt
     1801 gagcgggcat cggtgaccga cggctgcacg ggcggggtca ccaggcgatc gagcttggcc
     1861 gcggtgtcgg ccgcgacggc caggatgcgg cctgcgctgc gcagggcac
//
LOCUS       DAORNF010000680         1905 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_44258_length_1905_cov_1.185405, whole genome shotgun sequence.
ACCESSION   DAORNF010000680 DAORNF010000000
VERSION     DAORNF010000680.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1905
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44258_length_1905_cov_1.185405"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1104)
                     /gene="purH"
                     /locus_tag="PLV68_19895"
     CDS             complement(<1..>1104)
                     /gene="purH"
                     /locus_tag="PLV68_19895"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HRE03570.1"
                     /translation="AAAGLPVTDLAELTGYPAILGHRVVTLHPVIHGGLLADLHQPDH
                     VAELDQYGIEPFALAVVNLYPFVSEPGIELIDIGGPTLIRAAAKNHAHVGVVMDPDQY
                     DPVLAELRAGGALSAATRRALAAHAFAQIAAYDAEIATWFAQPDTTVAEPGSGDALPA
                     TLHLSLELAQPLRYGENPHQHGARYRRIGADSWWDHAVQHGGKELSYLNVYDTEAAWR
                     MVQRFDQPAVVIVKHANPCGVAVDADISQAYVRANACDPVSAFGGIVAVNRQVTLASA
                     EALAPVFTEVVVAPGYDDDALAVLTAKKNMRVLSAPAPTGAAFDVRSVDGGLLVQNAD
                     TVNLDRSTWKVVTEAQPTSEQWDDLAFAWVVCAG"
     assembly_gap    1105..1172
                     /estimated_length=68
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1526..1670
                     /estimated_length=145
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1671..>1905
                     /locus_tag="PLV68_19900"
     CDS             <1671..>1905
                     /locus_tag="PLV68_19900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03571.1"
                     /translation="SVISSSPRAEGFSARARSASFGMILSAISGLVAFAGAFLGFKES
                     GGDFNDLKDINKIKGQFGGGNGGGTPPPPPGGYG"
BASE COUNT          281 a          551 c          573 g          287 t
ORIGIN      
        1 cccgcgcaca ccacccacgc gaaggcgagg tcgtcccact gttctgatgt cggctgcgcc
       61 tcggtgacaa ccttccaggt ggaacgatcg aggttcaccg tgtcggcgtt ctgcaccaac
      121 aggccgccgt cgacgctgcg cacgtcgaac gccgccccag tcggtgccgg ggcggacagc
      181 acgcgcatgt tcttcttggc ggtcagcact gcgagcgcat cgtcgtcgta gccgggagcg
      241 acgacgacct cggtgaacac cggcgccagc gcttcggccg acgccagtgt gacctgacgg
      301 ttgaccgcga cgatgccacc gaacgcgctg acggggtcgc acgcgttggc acgcacgtac
      361 gcctgactga tgtccgcgtc gacggccaca ccgcacgggt tggcgtgctt gacgatcacc
      421 accgccggtt ggtcgaaccg ctgcaccatc cgccaggcgg cttcggtgtc gtacacgttg
      481 aggtagctca actctttgcc accgtgctgc accgcgtggt cccaccaact gtccgcaccg
      541 atgcgccggt aacgggcgcc gtgctgatgc gggttctcgc cgtagcgcaa cggctgggcc
      601 agttccagcg acaggtgcaa cgtcgccggc aacgcatccc cgctacctgg ctcggcaacg
      661 gtcgtgtcgg gctgggcgaa ccaggtggcg atctcggcgt cgtacgcggc gatctgggcg
      721 aaggcgtgtg cggccagcgc gcggcgggtc gcggccgaca gtgcaccgcc ggcacgcagt
      781 tcggccagca ccgggtcgta ctgatcgggg tccatcacga caccgacgtg ggcgtgattc
      841 ttggccgcgg cacggatcag cgtcgggcca ccgatgtcga tcaactcgat acccggctcg
      901 gagacgaacg ggtagaggtt gacgaccgcc agcgcgaacg gctcgatccc gtactggtcg
      961 agttcggcaa cgtggtcggg ctggtgcagg tcggccagca ggccgccgtg gatcacgggg
     1021 tgcagcgtga ccacgcggtg gccgaggata gccggatagc cggtcagctc ggccaggtcg
     1081 gtcaccggca atcccgcggc cgcgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngacttgtc gtacacggac agcaatgcag
     1201 taggcacgca ccgaagctag accgacccga accgccggcc actggccgtt caacgaaaag
     1261 tcaaccgttc atcaacgtct gcacaagagc cgcagtcatg cgcggtggcc gccgcgcggt
     1321 gatgtacggt gacgcggaca gcacgtcaca ggctctttgg gaggcacacg tggatttcag
     1381 caagttcaag acatccgatt ggttgaaggt aggtggtgca ctcggcttcc tgatcttcgg
     1441 cttcttcagc tggatcagtg tggactacga gggtgacacg ttcttcggcc gggccagtgg
     1501 caccagtggc aacgtcttcg atttcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tcggtgatct
     1681 cgagctcgcc ccgggccgag ggcttcagcg cgcgggcacg ctcggccagc ttcggcatga
     1741 tcttgtcggc catctccggg ctggtcgctt tcgctggcgc gttcctcggg ttcaaggagt
     1801 cgggtggcga cttcaacgac ctgaaggaca tcaacaagat caagggtcag ttcggcggtg
     1861 gcaacggtgg cggtaccccg ccgcccccgc ctggtggcta cggca
//
LOCUS       DAORNF010000681         1904 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_44281_length_1904_cov_1.875608, whole genome shotgun sequence.
ACCESSION   DAORNF010000681 DAORNF010000000
VERSION     DAORNF010000681.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1904
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44281_length_1904_cov_1.875608"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1904
                     /locus_tag="PLV68_19905"
     CDS             <1..>1904
                     /locus_tag="PLV68_19905"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03572.1"
                     /translation="FVADATLPTLHVVGQFSTGGAPYTWVTEFDVWVETASDVTPRRI
                     RQVVIGTDGRYEFDVVLPADSLSLTVTAEIGFGEDDWVTASLTSIDPGINRVDLVVAM
                     ELPVSADVTVGFLADDTTFSGLTRITTKLYDHRGVRIGGVTESLVTVAPDGSVTLALT
                     LPPAARAIEIAWRGTDAPTSGRLALEPGTNTLGTTLTATSVWVDVSGVLTESGVVQPN
                     TATTIRVQPLDWEANQAGPALLVPVTTDATGAYSLTAVLPPGTTVVELAWMPDWFRHP
                     QQRPAVRPGTNTLVVNLNRYPSNPLPQSIDVNGHLTAGGTPWPERVTIEVSFRRVADH
                     RVDRQTVEVTPDPLDGSFHFTANAPQPSQWVEFEPLFGEWGTNPVTRVEPTLVGVTPV
                     VLDVDLAGQIVRVSGTISGADQDWASQDWADMVIVVNPGAPNPSDTSVDWAWATVHPD
                     GSYDVWFHAPAGATEVAIGLWQPSKDDYVFAPTTYSLAGGAALTGVDIAVVLPSIAVT
                     GTVLRNGDPVETATFAVVWSRPPDPVLDEFLDLEWTEYVGVTPDDAGAYTLHAIGPSS
                     ATQATVTLLGLPVPVWVFLLVAASAFFVLRYTPFGRNIYAVGSNPEAARLSGINVGAT
                     IFGVYLISGF"
BASE COUNT          293 a          683 c          609 g          319 t
ORIGIN      
        1 gttcgtagcc gacgccaccc tgccgacgct gcacgtcgtc ggccagttca gcaccggtgg
       61 cgcgccgtac acatgggtga ccgagttcga cgtgtgggtc gaaaccgcga gcgacgtgac
      121 cccccgacgc atccggcagg tagtgatcgg cacagacggg cgctacgagt tcgacgtggt
      181 gctgcccgcc gattcgctct cgctgacggt caccgccgag atcggcttcg gagaagacga
      241 ttgggtgacc gcgtcgctga cgtcgatcga tccaggcatc aaccgtgtcg atctggtcgt
      301 cgcgatggaa ctaccggtgt cagccgacgt caccgtcggc ttcctggccg acgacaccac
      361 gttctccggc ctcactcgca tcaccaccaa gctgtacgac catcgcggtg tgcgcatcgg
      421 tggcgtgacc gaatcactgg tcacggtcgc tcccgacggc tcagtcacgc tcgcgctcac
      481 actgcccccg gcggcgcgcg cgatcgagat cgcatggcgc ggcaccgacg cgccgaccag
      541 cgggcgtctg gcgctggagc ctggcacgaa cacgctcggt acaacgctca ccgcgacgtc
      601 ggtttgggtc gacgtctccg gcgtcctgac ggaatcgggt gtggtccagc ccaatacggc
      661 caccaccatc agggtccagc cgctcgactg ggaggccaac caggccgggc ccgccctgtt
      721 ggtgcccgtg accaccgacg ccacgggcgc gtactcgctc actgcagtcc ttccgcctgg
      781 aacgaccgtg gtcgagttgg cgtggatgcc cgactggttc cgccatccgc aacagcggcc
      841 cgccgtcagg ccaggcacca acaccttggt ggtcaacctg aaccgctacc cgtccaaccc
      901 gttgccgcag tcgatcgatg tgaatggtca tctcaccgcg ggcggcacac cctggcccga
      961 acgggtgacg atcgaggtca gcttccgccg tgtcgccgac caccgcgtcg atcggcagac
     1021 ggtcgaggtc acgcccgacc cgctcgacgg ctcgttccac ttcaccgcca acgcgccaca
     1081 accatcgcag tgggtcgagt tcgagccact cttcggagag tggggcacca acccggtcac
     1141 ccgcgtcgaa ccaacgctgg tcggcgtcac gcccgtggtg ctcgacgtcg atctggcggg
     1201 gcagatcgtg cgtgtatccg gcaccatcag cggtgccgac caggactggg ccagccagga
     1261 ctgggccgac atggtgatcg tcgtcaaccc cggcgcgccc aacccgtccg acacgtcggt
     1321 cgactgggcg tgggcgacgg ttcatccgga tggcagttac gacgtgtggt tccacgctcc
     1381 ggccggcgcg accgaggtcg cgatcgggct gtggcaacca tcgaaggacg actacgtctt
     1441 cgcaccgacg acgtactctc tcgcgggcgg cgccgcgctg accggcgtcg acatcgcagt
     1501 ggtcctgccc tcgatcgcgg tgaccggcac agtgctgcgc aacggcgatc cggtcgagac
     1561 tgcgacgttc gccgtcgtct ggtcgcggcc tcccgacccg gtgctcgacg agttcctcga
     1621 tctggagtgg acggagtatg tcggcgtcac cccggacgat gcgggggcgt acacgctgca
     1681 cgcgatcggc ccttcgtcgg caacgcaggc gaccgtcacg ctgctcggcc tgccggtgcc
     1741 cgtgtgggtg ttcctgctcg tcgccgccag cgccttcttc gtgctgcgct acacgccctt
     1801 cggccgcaac atctatgcgg taggctccaa ccccgaagcc gcgcggctct cgggcatcaa
     1861 tgtcggcgcc accatcttcg gcgtctatct gatctcgggc ttcc
//
LOCUS       DAORNF010000682         1903 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_44319_length_1903_cov_1.512987, whole genome shotgun sequence.
ACCESSION   DAORNF010000682 DAORNF010000000
VERSION     DAORNF010000682.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1903
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44319_length_1903_cov_1.512987"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /locus_tag="PLV68_19910"
     CDS             complement(<1..456)
                     /locus_tag="PLV68_19910"
                     /inference="COORDINATES: protein motif:HMM:NF012708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HRE03573.1"
                     /translation="MTDTLLGILKYALLALLYLFFARVLWAVWSEVRGPRAASVRMRT
                     ADGVVAPIGQQRGAARPGKHANPGKPPKPAPVAKGRHGAVARLLVLEPSDSRGTALSV
                     ANEITFGRAPGCTVSVPDDAYVSQVHARVFNDRGDVTVEDLGSTNGTYVN"
     assembly_gap    522..641
                     /estimated_length=120
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(642..1096)
                     /locus_tag="PLV68_19915"
                     /pseudo
     CDS             complement(642..1096)
                     /locus_tag="PLV68_19915"
                     /inference="COORDINATES: protein motif:HMM:NF023818.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3662 domain-containing protein"
     gene            1514..>1903
                     /locus_tag="PLV68_19920"
     CDS             1514..>1903
                     /locus_tag="PLV68_19920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03574.1"
                     /translation="MRTPPVTASAMDVVDPVLRCPNCSGAVVVSERVVRCVAGHSFDR
                     SRSGHVNLLIGGRLARQGDKAAPGDSREMLVARQRFFDGGHYRPLMDRLAALATGAER
                     VVDIGCGVGSYLAHLAAPRRYGFDVAKT"
BASE COUNT          250 a          570 c          657 g          306 t
ORIGIN      
        1 gttcacgtag gtgccgttgg tgctgccgag gtcttcgacg gtgacgtcgc cccgatcgtt
       61 gaacacccgg gcgtgcactt gggacacgta ggcgtcgtcg ggaacgctga ccgtacaacc
      121 tggagcgcgg ccgaacgtga tctcattggc gacgctgagc gccgtgcccc ggctgtcgct
      181 cggttccagc accaacagcc gggccaccgc gccgtgacgg cccttggcca ccggtgccgg
      241 cttgggtggc ttgccggggt tggcgtgctt gcccggtctg gcggcgccac gttgttggcc
      301 gattggcgcc acgacgccgt cggcggtgcg catccgcacc gacgccgccc gcgggccgcg
      361 gacctcgctc cataccgccc acagcacgcg ggcgaagaac aggtacagca gcgccaacag
      421 cgcgtatttg aggatgccga gcagcgtgtc ggtcatatgt cggccgccct gggggcgctc
      481 aggtcgcctc gaaccggatg tgggtgctgc cgaaggtgac gnnnnnnnnn nnnnnnnnnn
      541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nggcggctga cgttggcgtc
      661 gttgagggga atgccggagt cggccatgcg gcccatgccg atgatggtgc cggtcaggat
      721 gatgcggctg ccgtcgggca gtgccaagcg gccaccgccg ttgcgggtgc tgacctcgcg
      781 catcgccgat gacaccgtga agcggccggg tcgggacgcc tcgtcgacca gcaggctgac
      841 ctgcaccgga cccttgaagt ggtagccctc ttcgcgggcg tgttcgcggg cggcctcgat
      901 caactcggtg accaacgcgt cctcgatgtc cgcgaacgcc gcgggggctg acgtggaact
      961 cgaacaggtt gggcacgatg cggcgaccct tgacgtcgac cgaacgatgg tcgtccatct
     1021 cccgcacgag acgccgaccg agctcgatcg ggcggatgct gcctttgctg gaacgcgcga
     1081 acacgccctc gaccatacgc tctacgccgt gctcgaacga ttgcagaccc atgccggtcg
     1141 caagagtagt ggtcagcagc cgcggtgaag ccgcaggtcg gatcagccgt tcgaccggtt
     1201 ttcgggtctg gtgttgcagg ttgggcggtg gcggcggagt tgctaccgtg tggcttccac
     1261 tcgggcgagt ggcggaatgg cagacgcgct ggcttcaggt gccagtgccc gcaagggcgt
     1321 gggggttcaa gtcccccctc cgacaccacc agtgttcgaa aggacgtgcg ggttcaagtc
     1381 ccgcctcgcc cacccagagt tgttcgcgaa accggagaga tctggtcgcg aaactcccgc
     1441 tctgaacggc ccgctggctt cggtcggcgg gtcgtcgagg tcggcgggtc gatcggcggc
     1501 atacgctcgc ctcatgcgga caccacctgt taccgcctcg gcgatggacg tggtggaccc
     1561 ggtgctgcgg tgtccgaact gctcgggggc tgtggtggtg tcggagcggg tcgtgcggtg
     1621 cgtggcgggg cacagcttcg accggagccg gagcggtcac gtcaacctcc tgatcggcgg
     1681 gcgtctggcg cgccagggcg acaaggcggc tcccggtgat tcgcgggaga tgctcgtcgc
     1741 ccggcagcgg ttcttcgacg gtgggcacta ccggccgctg atggatcgcc tggccgcgtt
     1801 ggccactggc gccgagcggg tggtcgacat cgggtgcggg gtcgggtcgt atctggccca
     1861 tctcgccgcg ccgcgtcggt acgggttcga cgtcgccaag acg
//
LOCUS       DAORNF010000683         1900 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_44395_length_1900_cov_1.819512, whole genome shotgun sequence.
ACCESSION   DAORNF010000683 DAORNF010000000
VERSION     DAORNF010000683.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1900
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44395_length_1900_cov_1.819512"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1769)
                     /locus_tag="PLV68_19925"
     CDS             complement(<1..1769)
                     /locus_tag="PLV68_19925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03575.1"
                     /translation="MARASGGDLTMPVDQIAPVELVGALARAILLAAEHALASSRPDE
                     AEDFAAIGLALATGTREIDLLDVRWGREAPDRGAAVDPSRPRMYRSACMPPNAVSPGT
                     QLDGWVEVPGRQVDWPLPPRLHRVLLALANDPPLPGSPVLAGRARTLSEADRLTTVMG
                     RLAGANGLSVGQIRLALATHLARKFGPEVAQLTFGDSFSMSVAPSYYASIPVESVFSA
                     VAGIQLHWFGEMADLQGDLSGKTLGSRLVLTDAAARRWPASLRQRIRSASHRAAGGIA
                     AQWRAWRNHLAGALCAATGARPTSWLEQIDLDQIVPEYGLVMLADKASDMLRQTRVAA
                     TGRRWISDLRLYLDWLIGRSSDLSDREIAAHAQRVLLSEAPLFSLPDTEGGPLTVAEL
                     RRSMPEPLQPFANHYRHRLNAVMQRQGVDPEIRHAQLGWIVTPAHAMADMSPWSAQSF
                     AKTLSPVLDQFLVDDGWYPASQRITQWSWAGVPDRPLRDWASVAREHVADHQREVRQI
                     KTRLVERWKEVADDVGERLGRAISRFFPSLRLDSRTRRLEPAEGFSGARLVEMSAGHH
                     GLLCDAVREGDMDPGDATEAVATR"
BASE COUNT          321 a          636 c          643 g          300 t
ORIGIN      
        1 cgcgtggcca ctgcctcggt ggcatcgccc gggtccatgt ccccctcgcg aaccgcatcg
       61 cacagaaggc catggtggcc tgccgacatc tcgacgagcc gggctcccga gaatccttcg
      121 gccggctcca gtctccgggt gcgcgaatcg agtcgcagcg acggaaagaa ccgggatatc
      181 gcacgaccga gacgttcgcc gacgtcgtcg gcaacttcct tccagcgctc gacgagccgg
      241 gtcttgatct ggcgcacttc gcgctggtgg tcggccacgt gctcccgagc gactgatgcc
      301 caatcacgaa gaggtcggtc tggaacgcct gcccaggacc actgcgtgat gcgctgactg
      361 gccggatacc acccgtcatc gaccaggaat tgatccagga cggggctcag ggtcttcgcg
      421 aatgactggg cactccaggg cgacatgtcc gccatggcgt gagccggcgt cacgatccag
      481 ccgagttgcg catgcctgat ctccggatcc actccctgtc gctgcatcac ggcgttgagg
      541 cgatggcggt agtggttggc gaagggctgc aggggttccg gcatggatcg ccgcaattcg
      601 gcgacggtca acgggccccc ttcggtgtcg ggcagcgaga acagcggcgc ttccgacagc
      661 aggacccgct gcgcgtgggc agcgatttct cgatcggaaa gatccgatga gcggccgatc
      721 agccagtcca ggtagaggcg caggtccgag atccatcgac gccccgttgc ggcgacgcgc
      781 gtctgcctga gcatgtcgct cgccttgtcg gccagcatga ccagcccgta ctcgggcacg
      841 atctggtcaa gatcgatttg ctccagccag gacgtgggtc gcgccccggt cgcggcgcac
      901 agggcgcccg cgaggtgatt tctccacgcg cgccattgcg cggcgatccc gccggccgcc
      961 cgatgcgatg ccgagcgaat ccgctgacgc agggaggcgg gccatcgacg cgcggccgcg
     1021 tcggtcagga cgaggcgtga accgagcgtt ttgccggaca ggtccccctg cagatcggcc
     1081 atctcgccga accagtgcaa ctggatcccg gctacggcac tgaacaccga ttccaccggg
     1141 atcgatgcgt agtaggacgg cgcgaccgac atggaaaagg agtcgccaaa ggtcaactgg
     1201 gcgacttcgg gtccgaactt ccgtgcgagg tgcgtcgcca gcgccagtcg gatctgcccg
     1261 acggaaagtc cgttggcgcc cgccaatcgg cccatgaccg ttgtgagccg gtcggcttcc
     1321 gacagagtgc gtgcccgtcc ggcgagcacc ggactgcccg gcagcggtgg atcgttcgcc
     1381 aacgcaagaa ggacccggtg caggcgcggc ggcaagggcc agtcgacctg cctgcccggc
     1441 acctcgaccc agccatccag ttgcgtgccg ggtgacaccg cgttcggggg catgcatgcg
     1501 gagcgataca tgcggggtcg tgacgggtcg acggctgcgc ctcgatcggg tgcctcgcgc
     1561 ccccacctca cgtcgaggag atcgatttcg cgtgtgccgg ttgcgagcgc caatccgatt
     1621 gcagcgaagt cctccgcctc gtcgggtcgg gacgaagcga gcgcatgctc tgcagcgagc
     1681 aggatcgcgc gagccagcgc accgacgagt tcgaccggtg caatctgatc caccggcatc
     1741 gtcaggtcgc cgccactcgc gcgggccatt cccctcgcca atgccttgcg acggcgaagg
     1801 cccggcgatc ggaacggacc gtcatccggc tcgaccaccg aagagtcgca ggcaatggct
     1861 gccggcggag atgagacgta tgcctttctt gcgaggcggt
//
LOCUS       DAORNF010000684         1894 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_44563_length_1894_cov_1.752039, whole genome shotgun sequence.
ACCESSION   DAORNF010000684 DAORNF010000000
VERSION     DAORNF010000684.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1894
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44563_length_1894_cov_1.752039"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>343)
                     /locus_tag="PLV68_19930"
     CDS             complement(<1..>343)
                     /locus_tag="PLV68_19930"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03576.1"
                     /translation="KGRDPGELAVTDPESLPKAVRDAIPYLRFRLNRVMLGRPMSSPE
                     QRARVGEDAMAVINEHPNAMTRTLYAGEVAAHTGLPATDLVRIAEQRSRRPAVRVAPG
                     RTRRVTAHAEFV"
     assembly_gap    344..358
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<359..1345)
                     /gene="dnaG"
                     /locus_tag="PLV68_19935"
     CDS             complement(<359..1345)
                     /gene="dnaG"
                     /locus_tag="PLV68_19935"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HRE03577.1"
                     /translation="MAIIDEDIEQIRAAVAIVDIVQPYVALRKSGRNWQGLCPFHAEK
                     SPSFNVSEQMARYHCFGCGASGDVFTFIQEIEHVDFVAAVEHLAAKAGITLRYTTGGE
                     SKQRQQRKRLVAAMGQAVDWYHQRLLTGSDARLARDYLRQRGLSGDIARQFKIGWAPD
                     DWDALSRESGVAADSLRDVGLGFLNKRNRMQDSFRARVLFPIFTDTGEPVAFGGRILP
                     GSSDPAKYKNSAETAIYAKSRTLYGLNWAKTDIVQHNEVVVCEGYTDVIGFHIAGVPR
                     AVATCGTALTEQHIQMLKRFASRVVLAFDADNAGQGAAERVYEWEQKFQVEFS"
     assembly_gap    1371..1656
                     /estimated_length=286
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1657..1873)
                     /locus_tag="PLV68_19940"
     CDS             complement(<1657..1873)
                     /locus_tag="PLV68_19940"
                     /inference="COORDINATES: protein motif:HMM:NF014895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03578.1"
                     /translation="MLLIGTMIETPRAALVADRMARVADFFSFGTNDLTQMTFGFSRD
                     DIESKMMPAYLEQGLLKRNPFETIDITG"
BASE COUNT          242 a          529 c          548 g          274 t
ORIGIN      
        1 cgacgaactc ggcgtgggcg gtgacccgcc gggtccggcc cggcgccacc cgcacggccg
       61 ggcgacggga acgctgctcg gcgatgcgca ccaggtcggt ggcgggcaga ccggtgtggg
      121 ccgcgacctc gcccgcgtag agcgtgcggg tcatcgcgtt ggggtgctcg ttgatcaccg
      181 ccatcgcgtc ctcgccgacg cgcgccctct gttcgggcga cgacatcggc cgcccgagca
      241 tcacccggtt gagacggaaa cgaaggtacg ggatggcgtc gcgcaccgcc ttgggcaacg
      301 actcgggatc ggtgacggcc agctcaccag ggtcgcgtcc cttnnnnnnn nnnnnnnngg
      361 aaaactcgac ctggaacttc tgctcccact cgtagacccg ctcggccgcg ccctgaccgg
      421 cgttgtcggc gtcgaaggcc agcacgaccc ggctggcgaa gcgcttcagc atctggatgt
      481 gctgctcggt caacgccgtg ccacaggtgg cgaccgcacg cggcaccccg gcgatgtgga
      541 acccgatcac gtcggtgtag ccctcgcaga ccaccacctc gttgtgctgc acgatgtcgg
      601 tcttggccca gttgagccca tagagcgtgc ggctcttggc atagatcgcc gtctcggccg
      661 agttcttgta cttggccggg tcgctcgatc cgggcaggat gcgaccgccg aatgcgacgg
      721 gctcgcctgt atcggtgaag atcgggaaca gcacgcgggc gcggaagctg tcctgcatgc
      781 gattgcgctt gttgaggaac ccgagcccga catcgcgcag gctgtcggcg gccacgccgg
      841 actctcggct gagcgcgtcc cagtcgtcgg gtgcccagcc gatcttgaac tgccgtgcga
      901 tgtcgccgct gaggccgcgc tggcgcaggt agtcgcgcgc cagacgggcg tcgctgcccg
      961 tgagcagccg ttggtgatac cagtcgacgg cctgtcccat cgccgccact aaccgcttgc
     1021 gttgctgtcg ctgcttcgac tcaccaccgg tggtgtagcg cagtgtgatg ccagctttgg
     1081 cagccaggtg ctcgacggcg gcgacgaagt cgacgtgctc gatctcttgg atgaaggtga
     1141 acacgtcccc cgacgcgcca cagccgaagc agtggtagcg ggccatctgc tcgctgacgt
     1201 tgaacgacgg gctcttctcc gcgtggaagg ggcacagccc ctgccagttg cggccggact
     1261 tgcgcagcgc gacgtacggc tggacgatgt cgacgatcgc gaccgcagcg cggatctgct
     1321 cgatgtcctc gtcgatgatg gccacgccga gacgttaccg gcgtggcccg nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncgcc ggtgatgtcg atggtctcga
     1681 acgggttgcg cttgagcaga ccctgctcga ggtaggccgg catcatcttc gactcgatgt
     1741 cgtcacggct gaacccgaag gtcatctggg tcaggtcgtt ggtgccgaag ctgaagaagt
     1801 cggcgaccct ggccatgcgg tccgcgacca gcgccgcacg cggcgtctcg atcatggtgc
     1861 cgatcagcaa catcgaggtg atgccgcgct cctc
//
LOCUS       DAORNF010000685         1893 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_44600_length_1893_cov_1.773667, whole genome shotgun sequence.
ACCESSION   DAORNF010000685 DAORNF010000000
VERSION     DAORNF010000685.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44600_length_1893_cov_1.773667"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..565)
                     /locus_tag="PLV68_19945"
     CDS             complement(<1..565)
                     /locus_tag="PLV68_19945"
                     /inference="COORDINATES: protein motif:HMM:NF019346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRE03579.1"
                     /translation="MITHTPDLVAAEHPLATARRVSTALRDSLGAVMAGRSQQIDVTV
                     ATVLAGGHVLIEDVPGVGKTLLAQALAAAIGGSFARVQGTADLLPSDVIGSLTPDADG
                     RGLHFRPGPLFANVVLFDELNRANARTQSALLEVLEEGHVTVDGVAYAVPSPFVLVAT
                     QNPVRMAGTYPLAEGVTDRFTAALSLGR"
     gene            653..1804
                     /gene="dusB"
                     /locus_tag="PLV68_19950"
     CDS             653..1804
                     /gene="dusB"
                     /locus_tag="PLV68_19950"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HRE03580.1"
                     /translation="MLSTAVDVAPLQLGQHSVWPPVVLAPMAGVTNAAFRTMCRRFGP
                     GLVYVNEMVMATALVHGSAKTDRMATFGPGDTFRSLQVYGSDPVMVGEAVRRLVDADR
                     VDHIDLNFGCPANKVTRRGGGAAVPVKPNLLRAILRAVVVAASPAGVPVTAKFRMGID
                     DDLLTFVRTGQIAEDEGLAAIALHARTAQQHYAGEARWDAIGELKAAVSSIPVLGNGD
                     IWEAADAVAMIDQTGCDGVVVGRGCLGRPWLFGNLIAAFSGAEVPPSPPLGFVAQQMA
                     DHCRLLADLVGEDNAVRDFRKHTSWYLTGYPVGGETRGRFSRIASLAELDDLLAALDP
                     TMTVVPGGERIRRGHTNGPIRVVLPHGDLDDLDDATPPDDGDVTALSGG"
BASE COUNT          274 a          636 c          682 g          301 t
ORIGIN      
        1 cacggcccag gctgagcgcg gcggtgaagc ggtcggtcac tccttccgcc aagggatagg
       61 tgccggccat tcgcaccggg ttctgcgtcg cgacgagcac gaacgggctc ggcaccgcgt
      121 aggccacacc gtcgaccgtg acgtggccct cctccagtac ctccaacagc gccgactggg
      181 tgcgggcgtt ggcccggttc aattcgtcga acagcacgac gttggcgaac agtgggccgg
      241 ggcggaagtg cagcccgcgg ccgtcggcat cgggcgtcag cgagccgatc acgtcggacg
      301 gcagcaggtc ggcggtgccc tggacgcggg cgaacgatcc accgatcgcg gctgccagcg
      361 cctgcgcgag cagcgtcttg ccgacgccgg gcacgtcctc gatcaacacg tggccaccgg
      421 ccagcacggt ggccacggtg acgtcgatct gctgtgaacg gccggccatg acggcgccga
      481 gcgagtcgcg cagcgcggtg ctcacccggc gggcggtggc gagggggtgt tcggccgcga
      541 cgaggtcagg tgtgtgcgtg atcatgcgcc tcagtcatcg gctcgcgatg ggtgtttctc
      601 aagcaaccgg gccgaacaca cgtcgcggat gcccacagat agcgttggtc agatgctgtc
      661 cactgccgtc gacgtcgcgc cgttgcaact ggggcagcac agcgtgtggc caccggtggt
      721 attggcgccg atggcaggcg tgaccaacgc tgcgttccgc acgatgtgcc gccgtttcgg
      781 gccgggcctg gtgtacgtca acgagatggt catggcgacc gcgctggtgc acggcagcgc
      841 caagaccgat cgcatggcca ccttcggtcc tggcgacacg ttccgcagct tgcaggtcta
      901 cggcagcgac ccggtgatgg tgggcgaggc ggtgcgccgc ctggtcgatg ctgaccgggt
      961 cgatcacatc gatctcaact tcgggtgccc ggccaacaag gtcacccgtc gcggcggtgg
     1021 tgccgcggtg ccggtcaagc ccaacctgtt gcgagcgatc ctgcgcgccg tggtcgtggc
     1081 ggcatcgcct gccggggtgc cggtgacggc caagttccgg atgggcatcg acgacgactt
     1141 gctcaccttc gtgcgcaccg gccagatcgc cgaggacgag ggcttggccg ccatcgcgct
     1201 gcacgcccgc accgcgcagc agcactatgc gggtgaggcg cgctgggacg cgatcggcga
     1261 actcaaggcc gcggtgtcgt ccattcccgt gctcggcaat ggcgacatct gggaggcggc
     1321 cgatgcggtc gcgatgatcg accagaccgg atgcgacggc gtggtggtcg ggcgcgggtg
     1381 cctcgggcgc ccgtggttgt tcggcaacct gatcgccgcg ttcagcggtg ctgaggtgcc
     1441 gccgtcaccc ccgctcggtt tcgtcgccca acagatggcc gaccattgcc ggttgctggc
     1501 cgatctggtc ggcgaggaca acgccgtgcg cgacttccgc aagcacacct cgtggtacct
     1561 caccggctac ccggtcggcg gtgagacccg cggtcggttc agccgcatcg ccagtctggc
     1621 cgagctggac gacctgctcg ccgcgctcga tccgacgatg accgtggtgc ccggtggcga
     1681 gcgcatccgc cgcggtcaca ccaacggccc gatccgggtg gtgctgcctc acggcgacct
     1741 cgacgacctc gacgacgcga ccccgcccga cgacggtgac gtcaccgcct tgagcggtgg
     1801 ctgaggcttc gttgcagatg caacaaggtg accgcgtggt gcgtgtggtg ttggcatccg
     1861 cgtcgccgcg ccgtcgcgag attctggccg acc
//
LOCUS       DAORNF010000686         1890 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_44684_length_1890_cov_2.342779, whole genome shotgun sequence.
ACCESSION   DAORNF010000686 DAORNF010000000
VERSION     DAORNF010000686.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1890
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44684_length_1890_cov_2.342779"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1069
                     /locus_tag="PLV68_19955"
     CDS             <1..1069
                     /locus_tag="PLV68_19955"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRE03581.1"
                     /translation="PSRGLRTPRQEQELATTDITEIAPGVLRSQLPIDVPGLGHVNCY
                     LLEDERGVAVVDPGMPDAASWDALVDRLARAGYTPANVHTAIVTHSHPDHFGGAIRLQ
                     REFGADVVSHESFRTIFQSDGSEDSADLEVNSDDSMIEAIERYIRRPAPWGGERPGPP
                     PEFFALLRSGQVVGDHMTERLTPTVRLVDSQVVRFARREWVAMHTPGHTHDHLCLFDP
                     EHGVVFTGDHVLPSITPHISGIADHHDPLALFVQSQQRMIDMTGVTIALPAHGHPFTD
                     LAGRAAHIIEHHDERLNLIRGARGDLPNGTVHDFMQVLFQPRSWGGMAESETYAHLEH
                     LRELGEMRRTDEAGIAHYSAV"
     gene            complement(1101..1607)
                     /locus_tag="PLV68_19960"
     CDS             complement(1101..1607)
                     /locus_tag="PLV68_19960"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRE03582.1"
                     /translation="MDRTVRLDRVEIETITGRADLAPLLARWHAAEWAHLYDPSVWNE
                     QIAVEEFVAQAAPGSDDVTWIAFDGPGRGHGDVVGSVSLLDSDDLTGYEHLSPWLASL
                     YVRPDWRGVGIARRLIDTLIEAARVRGDQRVHLFTSGQEGYYLDRGWHIVGGSGVKTV
                     MTIETDAI"
     gene            complement(1620..>1890)
                     /locus_tag="PLV68_19965"
     CDS             complement(1620..>1890)
                     /locus_tag="PLV68_19965"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRE03583.1"
                     /translation="EAAGMGLANLAVPADQVLSTAREWAGEIAANAPLAVQSMKRLFR
                     HGMTEDFPSHVHHVLLQTMLLFRSKDFTEGITSFMEGRAPNFTGR"
BASE COUNT          307 a          691 c          607 g          285 t
ORIGIN      
        1 cccctcacgc ggcctgcgca ccccccggca agagcaagaa ctcgccacga ccgacatcac
       61 cgagatcgcc ccgggcgtgt tgcgcagtca gttgccgatc gacgtacctg gcctcggcca
      121 cgtcaactgc tacctcctgg aggacgagcg cggcgtggcc gtggtcgacc ccggcatgcc
      181 cgacgccgcg tcgtgggacg cgttggtcga tcgcctcgcc cgggcgggct acacgcccgc
      241 caacgtccat accgcgatcg tcacccactc ccaccccgac cacttcggcg gagcgattcg
      301 gctgcagcgc gagttcggag cggacgtcgt cagtcatgag tcgttccgca cgatcttcca
      361 gagcgatggc tctgaggact cggccgacct ggaggtcaac tccgacgaca gcatgatcga
      421 agcgatcgag cgctacatcc gccgccccgc gccgtggggt ggcgagcgac ccggaccgcc
      481 gcccgagttc ttcgcgctgt tgcgctccgg ccaggtcgtc ggcgaccaca tgaccgagcg
      541 gctcaccccc accgtgcggc tggtcgacag ccaggtcgtg cgcttcgctc gccgcgagtg
      601 ggtggcgatg cacacgccgg gccacaccca cgatcatctc tgcctgttcg atcccgaaca
      661 cggcgtggtg ttcaccggcg accacgtcct gccgtcgatc accccccaca tcagcgggat
      721 cgccgaccac cacgacccgc tcgccttgtt cgtgcagtcg caacagcgga tgatcgacat
      781 gaccggggtg accatcgccc tgcccgccca cggccacccg ttcaccgatc tcgccgggcg
      841 agcggcgcac atcatcgagc atcacgacga gcggctcaac ctgatccgcg gcgcgcgggg
      901 cgacttgccc aacggcaccg tgcacgactt catgcaggtg ctgttccagc cccgctcgtg
      961 gggtggcatg gccgagagcg agacctacgc ccacctcgag cacctgcgcg aactgggcga
     1021 gatgcgccgc accgacgagg ccggcatcgc ccactacagc gccgtctgac ccgtgggcgg
     1081 tcggatgcga tctgctgcct tcagattgcg tcggtctcga tcgtcatcac ggtcttgacg
     1141 ccgctgccgc cgacgatgtg ccagccgcgg tcgaggtagt agccctcctg accgctcgtg
     1201 aaaaggtgga cgcgctgatc accacgaacg cgcgccgcct cgatcaaggt gtcgatcagc
     1261 cgccgggcga tgccgacgcc gcgccagtca gggcgcacgt acaggctggc cagccagggc
     1321 gagaggtgct cgtagccggt caggtcgtcg gagtccaaca gcgagaccga gccgaccacg
     1381 tcgccgtgac cgcggccagg cccgtcgaag gcgatccatg tgacgtcgtc agagccgggt
     1441 gctgcctgcg cgacgaactc ctcgacggcg atctgttcgt tccagacgga aggatcgtag
     1501 aggtgggccc attccgcggc atgccatcgg gccaacagcg gcgcgaggtc ggcccggccg
     1561 gtgatcgtct cgatctcgac ccggtcgagc cgcacggtgc gatccacacc gggcaccgct
     1621 cagcgcccgg tgaagttcgg tgcccgccct tccatgaacg acgtgatgcc ctcggtgaag
     1681 tccttgctgc ggaacagcaa catcgtctgc agcagcacgt ggtggacgtg gctcgggaag
     1741 tcttcggtca tgccgtggcg gaagaggcgc ttcatcgact gcacggcgag cggtgcgttg
     1801 gcagcgatct cacccgccca ctcgcgcgcc gtcgacagca cctgatcggc cggcaccgca
     1861 aggttggcca agcccatgcc cgccgcctcg
//
LOCUS       DAORNF010000687         1883 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_44901_length_1883_cov_1.752735, whole genome shotgun sequence.
ACCESSION   DAORNF010000687 DAORNF010000000
VERSION     DAORNF010000687.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1883
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44901_length_1883_cov_1.752735"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..490
                     /locus_tag="PLV68_19970"
     CDS             <1..490
                     /locus_tag="PLV68_19970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03584.1"
                     /translation="EVEREVVADGLPAWVVRLVSALHARRAVLITFNYDRIIERALME
                     LDLHDFDQPSGDHLVSWIDMFGDVPSYPPIPMRFSGGLKPTIRLLKLHGSLNWYWVPG
                     DSSGATLHHWELDDDTEGRARYLPRSRAVRRTARRDQVSLLPEPNHGRDVEASGRSAS
                     SR"
     gene            444..1118
                     /locus_tag="PLV68_19975"
     CDS             444..1118
                     /locus_tag="PLV68_19975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03585.1"
                     /translation="MAETWRRAAGALRAADSVALVGYSLPVADLVAAGMISETVAREG
                     VAVTVVNPTPGPVAESVLRLTGRTAQQCLSVEEFAASYVDSASKDLANQLIDFGTTAP
                     ADTLMLVGWNGDLLARVVDVERNDRGVEVVVAEFDDATPFAPVPEDSTRRAARPLSDL
                     VRQLSLGDTVEAVFAGGSRSTLVAIDRCKTTTGASVNWQILIPADRPRDIGVTSWRQK
                     LGLEAS"
     gene            1133..>1883
                     /locus_tag="PLV68_19980"
     CDS             1133..>1883
                     /locus_tag="PLV68_19980"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HRE03586.1"
                     /translation="MVQKLWNFCNVLRDDGVSTIEYVEQLTYLLFLKMADERQKAGLG
                     EVVPVGLDWQSLLDRDGDELEVHYRHILESLGRQTGTLGTIYRKAQNRIQDPAKLRRL
                     VVNLINEETWTAVDADLKGDAYEGLLQKGAEDTKSGAGQYFTPRPLVRAMMACLRPKP
                     GEKVADPACGTGGFLLAAHEWIVDHHISEMDADDKRFLRDEAMHGWEIVDGTARLCAM
                     NLLLHGIGTPSGKSLVTVADALRGDPGLRVDV"
BASE COUNT          331 a          562 c          633 g          357 t
ORIGIN      
        1 cgaagtcgag cgggaggttg tcgctgacgg cctcccagct tgggtggtgc ggctcgtatc
       61 ggcccttcac gctcgtcggg cggtcctgat caccttcaac tacgaccgca tcattgagcg
      121 cgcgctgatg gaactggacc tgcacgactt cgaccagccg tcgggggatc acctcgtctc
      181 gtggatcgac atgttcggcg atgtcccgtc ctaccccccg atcccaatgc gattctcggg
      241 cgggctcaaa ccgacgattc gtctcctgaa gctgcacggg tcgctgaact ggtactgggt
      301 gccgggagat tcctccggcg caacacttca ccattgggag ttggacgacg acactgaggg
      361 tcgtgcgcgc tacctccccc ggtcgcgagc cgttcgtcgt accgcccgcc gcgaccaagt
      421 cagccttctt ccggaaccca atcatggccg agacgtggag gcgagcggcc ggagcgcttc
      481 gagccgctga ctcggtggct cttgtcggct actcgctgcc cgtcgcggac cttgtcgctg
      541 ccggcatgat cagcgagacc gtcgcccgcg agggtgtcgc agtgacggtc gtcaacccga
      601 cgccaggacc tgttgcggag tcggtacttc gccttacagg gagaactgcg cagcagtgcc
      661 tctccgtaga ggagttcgcc gcctcctacg tggactcggc ttcgaaggat ttggcaaatc
      721 aactcatcga cttcggtaca actgctccag cggacacact gatgcttgtc gggtggaacg
      781 gtgacctcct tgcgcgagtt gttgacgtgg agcggaatga ccgaggtgtt gaagtcgttg
      841 tggccgaatt cgacgacgcc acaccattcg cgcctgtgcc ggaggattcg actcgaaggg
      901 cagcgcgtcc gctgtcggac ctcgtgcgac agctgagcct cggtgacact gtcgaggcgg
      961 tattcgccgg cggttcccgg agcactcttg tagcgatcga tcggtgtaag acgaccaccg
     1021 gcgcgtcggt gaactggcag attctgatcc cggcggatcg tccccgggac atcggcgtga
     1081 cgagctggcg tcagaagcta ggcctggagg catcgtgagt gggtcgaatc agttggtaca
     1141 gaagttgtgg aacttctgca acgtgctccg cgacgacggg gtgtcgacga tcgagtacgt
     1201 cgagcaactc acgtacctgc tgttcttgaa gatggcggac gagcgccaga aggctggact
     1261 cggcgaggtc gtaccggtcg ggctcgattg gcaatcgctg ctcgaccgcg acggcgatga
     1321 actggaggtg cactaccggc acatcctgga gtcgctcggg cggcagacgg gaacgctcgg
     1381 aacgatctac cgcaaagccc agaaccgcat ccaggacccg gccaaacttc gccggctcgt
     1441 cgtgaacctg atcaacgagg agacgtggac cgcggtcgac gccgacctga agggcgatgc
     1501 gtacgagggg ctgctgcaga agggcgctga ggacacgaag tcgggtgccg gccagtactt
     1561 cacccctcgt ccgttggtac gggcgatgat ggcgtgcctg cgtccgaagc cgggcgagaa
     1621 ggtcgctgat ccggcctgcg gtaccggtgg cttcctcttg gctgcgcacg agtggatcgt
     1681 cgatcaccac atctctgaga tggacgcaga cgacaagcga ttcttgcgcg acgaggcgat
     1741 gcatggttgg gagatcgtcg atggaacagc tcgcctgtgt gcgatgaatc tgttgttgca
     1801 cggcatcggc acgccgagcg gtaagagcct cgtgacggtc gccgatgcgt tgcgcgggga
     1861 tccgggcttg cgcgtggacg tga
//
LOCUS       DAORNF010000688         1881 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_44959_length_1881_cov_1.493976, whole genome shotgun sequence.
ACCESSION   DAORNF010000688 DAORNF010000000
VERSION     DAORNF010000688.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1881
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44959_length_1881_cov_1.493976"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>249)
                     /locus_tag="PLV68_19985"
     CDS             complement(<1..>249)
                     /locus_tag="PLV68_19985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03587.1"
                     /translation="AHPPQHLLHVYGLGDTYTPPSTIESFARAMRLPIAAPVLKNLGT
                     AFPVVQPPLRDNLELPGGRVTVALVQVQPTDYDGHFVL"
     assembly_gap    250..402
                     /estimated_length=153
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<403..>693)
                     /locus_tag="PLV68_19990"
     CDS             complement(<403..>693)
                     /locus_tag="PLV68_19990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03588.1"
                     /translation="NFANPRAARGNLYQGAADVFALVHAFRGLEISAGQSPTGSRIAF
                     DATRILYVGHSQGATTGPLATPYEPDVRAVVWSGAGGGLVLSLLSKKSPVDIP"
     assembly_gap    694..743
                     /estimated_length=50
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<744..>1881)
                     /locus_tag="PLV68_19995"
     CDS             complement(<744..>1881)
                     /locus_tag="PLV68_19995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03589.1"
                     /translation="RGVPAADGDFFRLTLPNDIRMKGGRLDLAGFPTPGPGVVGFDPV
                     DRVARAAEAVQRGFSMNPAVLFRLSRGFDLGTVWADGISNPPPGLPTLYFVNIDKDSP
                     QYGWTPDYGYFVTDGGGKYICPRFIAVHPAWYAPLLPDTTYAVILADGIATGDGTKFQ
                     ADGDMVAMLSEAAPQGDATLRLAWDAYAPLRAYLADDAPVNASRVIAAAVFTTQKVND
                     VLPAIREAIKATTPQRPTSIVRCAAGTTSPCDDGLAGDKHVRGCFGESEVAHELHMKV
                     ALPRVQAGTRPYLRPEDGGDLVFGGGKPVLQGTEEVCVSLTVPKNVAMPAAGWPLAIY
                     GHGTGGTFRSSVRDAGLVLADVTVPATTHPPAAETRLGVAVLGWD"
BASE COUNT          306 a          651 c          476 g          245 t
ORIGIN      
        1 acagcacgaa atgaccatca taatcggtcg gctgcacctg gacgagcgcc acggtcaccc
       61 tccctcccgg cagctcgagg ttgtcacgca gcggcggctg aactacgggg aacgccgtcc
      121 cgaggttctt cagcaccggt gccgcgatgg gcagccgcat cgcccgcgcg aagctctcga
      181 tggtcgaagg cggcgtatat gtatcgccaa gcccatacac atgcagcaga tgctgcggcg
      241 gatgcgccgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngggatgtc caccggcgac
      421 ttcttcgaca acagcgacag caccaaccca ccgcccgctc ccgaccacac gaccgccctc
      481 acatccggct cgtacggtgt cgcgagcggc cccgtcgtcg ccccctgcga gtggccgaca
      541 tagagaatcc gcgtcgcatc gaacgcgatt cgactgccgg tcgggctctg ccccgccgag
      601 atctcgaggc cccgaaaggc atgcaccaac gcgaacacat cggccgcccc ttgatacaaa
      661 ttgcctcgcg ccgccctcgg attcgcgaag ttgnnnnnnn nnnnnnnnnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nnncgtccca ccccagcact gccaccccga gccgcgtctc
      781 ggccgcgggc gggtgcgtcg tagcgggcac agtcacgtcg gccaacacca accccgcatc
      841 gcgcacagac gacctgaagg tccctcccgt gccatgccca tagatcgcga gcggccaccc
      901 cgccgccggc atcgccacat tttttggcac cgtcagcgag acacagacct cttcggtccc
      961 ctgcaacacg ggcttgccac caccaaacac caggtcgcca ccatcctcgg gtctcaagta
     1021 cggccgcgtc ccggcctgca ctctcggcag cgccaccttc atgtgcagct cgtgcgcgac
     1081 ctcactctca ccgaaacagc cccgcacgtg tttgtcgccg gccagcccat catcgcatgg
     1141 cgaggtcgtc cccgccgcgc acctcacgat ggacgtcggc ctctgcggcg tcgtcgcctt
     1201 gatggcctcg cgaatcgccg gcaagacatc gttcaccttc tgggtcgtga agaccgccgc
     1261 cgcgatgacc cgcgacgcgt tcaccggcgc atcgtcagcc aggtaggccc gcagcggcgc
     1321 atacgcatcc caagccaacc tgagcgtcgc atcaccttgc ggcgccgcct cgctcaacat
     1381 cgccaccatg tcaccatccg cctggaactt cgtcccatcc ccggtcgcaa tcccatcggc
     1441 gagaatgacc gcgtaggtcg tgtccggcag aagcggcgcg taccacgcgg gatggaccgc
     1501 gatgaagcgt gggcagatgt atttgccgcc accatcggtc acgaaatacc catagtccgg
     1561 cgtccaccca tactgcggcg agtccttgtc gatattgaca aaatacaagg tcggcagccc
     1621 cggcggcggg ttcgagatcc catcggccca caccgtcccg aggtcgaacc cccgcgagag
     1681 ccggaacagc accgccgggt tcatcgagaa cccccgctgc accgcctcgg ccgcacgcgc
     1741 cacgcggtcg accgggtcga acccgaccac cccgggcccc ggtgtcggga accccgcgag
     1801 gtcgagccgc ccgcccttca tgcggatatc attgggcaac gtcagccgga agaagtcgcc
     1861 atcggccgcc ggcaccccgc g
//
LOCUS       DAORNF010000689         1870 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_45277_length_1870_cov_1.825344, whole genome shotgun sequence.
ACCESSION   DAORNF010000689 DAORNF010000000
VERSION     DAORNF010000689.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1870
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45277_length_1870_cov_1.825344"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="PLV68_20000"
     CDS             complement(<1..260)
                     /locus_tag="PLV68_20000"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HRE03590.1"
                     /translation="MVVVPAWLEMAEETAIDGVTAISIEPGATFGLGDHPTTVTTMAA
                     MRRVLRAGDRVRSYLAEVRAESRGPQIFMSRTAPEFMMELFKL"
     gene            complement(565..1083)
                     /locus_tag="PLV68_20005"
     CDS             complement(565..1083)
                     /locus_tag="PLV68_20005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HRE03591.1"
                     /translation="MPIYALGDQVPDIHPDAYVHPDAVVIGSVFVGANSSIWPCAVLR
                     GDDGEIHVGERSSIQDGCVVHTTPFTFTRVDCVIGPLVHLEGCTIFDGALVGNGAIVM
                     HNVEVHTGALVAANTVLLNNTVVPAGALAAGVPAVIKEGRAVALAGQISFGSKSYVQR
                     AERFTRELRRID"
     gene            1166..>1870
                     /locus_tag="PLV68_20010"
     CDS             1166..>1870
                     /locus_tag="PLV68_20010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03592.1"
                     /translation="MSRRIEIELTSARPDGTWTDVVQLGSGMRNNQLITAPDGQVWLT
                     AVSGAVRLWRQSGEGWAGSEQIPGTEGVNQFSLIAGPGGRLDFLWCGQTGAVNYIRRS
                     GGVWSAKTQIGGGRPNMGGCEFEMATTPDGSVHVIWAEPDGGDHHTVYRRRAPDGSWG
                     AVTILHEGGPGSLVSDAGGRVHLIRPGYEPNNFGKWAWQYRQWNEGVWGLEETLGFYK
                     NPNLLSKVMAAADGAVQ"
BASE COUNT          321 a          584 c          658 g          307 t
ORIGIN      
        1 agcttgaaca gctccatcat gaattcgggc gcggtgcgcg acatgaaaat ctgcggtccg
       61 cgcgactccg cacgcacttc ggccagatag ctgcgcacgc ggtcgccggc acgcaacacc
      121 cggcgcatcg ccgccatggt ggtcaccgtg gtggggtggt cgccgaggcc gaaggtggct
      181 cccggctcga tcgagatggc ggtcacgccg tcgatcgccg tctcctccgc catctccagc
      241 cacgctggca ccacgaccag gtcgggttcg acccacgtcg ggcgggcgaa gtcgcgccag
      301 gtgtcggcga cggcggtgtc gatcggttcg aaccgccagg ggtagccgaa ctgggccagt
      361 tctgccgtga tcgcctcggt gtcgtcgccc agcgacgtcc acagttcgac gtgctcgggc
      421 tgtccggcga tggcggcgcg ctcttcgatc gcgacgacgc cgagcgacgg tcggaggcca
      481 cctcgacctc ggctgcatcg acgctgacga ccagcgccag cgtcacgctc gtcggctgcg
      541 tcagagccgg ctgttcgtcg ggcatcagtc gatgcggcga agctcgcgcg tgaagcgctc
      601 tgcgcgctga acgtagctct tcgacccgaa cgagatctgt ccggccagcg cgactgcccg
      661 accttccttg atcaccgcgg gtaccccggc cgcgagcgcg ccggccggga ccacggtgtt
      721 gttgagcaac acggtgttgg ccgcgacgag cgcgccggtg tgcacctcga cgttgtgcat
      781 cacgatcgcc ccgttgccga ccagggcgcc gtcgaagatg gtgcaccctt cgagatgaac
      841 gaggggaccg atcacgcagt cgacgcgggt gaaggtgaac ggggtggtgt gtaccacgca
      901 gccgtcttgg atcgacgacc gctcgccgac atggatctcg ccgtcgtcgc cgcgcaacac
      961 cgcacacggc cagatcgaac tgttggcgcc gacgaacacc gacccgatca cgaccgcatc
     1021 cggatgcacg taggcgtctg ggtggatgtc aggcacttgg tcgcccaggg cgtagatcgg
     1081 catccgtcca ccgtaccgat ctgcctggcg agcagtgcaa agcgggtgga agagtcaggc
     1141 ctcgacgcgg atagcgtggc gtctcatgtc ccgcaggatc gagatcgagt tgaccagtgc
     1201 gcgccccgac ggcacgtgga ccgacgtcgt ccaactgggc agcgggatgc gaaataacca
     1261 gctgatcacc gcgcccgatg gtcaggtctg gctgacggcc gtgagtgggg ccgtccggct
     1321 gtggcgtcaa tccggcgaag gttgggcggg gagtgagcag atccccggaa ccgagggggt
     1381 gaatcaattc agcctgatag ccggaccggg cggccgactg gacttcctct ggtgcggtca
     1441 gacgggtgcg gtcaattaca tccggcggtc gggcggggtc tggtcggcga aaacgcagat
     1501 cggcggtggc agaccaaata tgggggggtg tgagtttgag atggcgacga cgcccgacgg
     1561 atcggtccat gtcatctggg ccgaaccgga cggcggtgac caccacaccg tttatcggcg
     1621 gcgcgcccct gacggctcct ggggggcagt cactattctt cacgagggtg gcccgggcag
     1681 tctggtatcg gatgcgggcg gccgcgtcca tctcatccgg ccaggctatg agccgaacaa
     1741 ctttggcaaa tgggcatggc aatatcggca atggaatgag ggcgtgtggg ggttagagga
     1801 aacattgggc ttctacaaaa accccaacct tctttcgaag gtgatggcgg cggccgacgg
     1861 cgcggtgcag
//
LOCUS       DAORNF010000690         1869 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_45313_length_1869_cov_1.377067, whole genome shotgun sequence.
ACCESSION   DAORNF010000690 DAORNF010000000
VERSION     DAORNF010000690.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1869
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45313_length_1869_cov_1.377067"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    217..382
                     /estimated_length=166
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<383..802)
                     /locus_tag="PLV68_20015"
     CDS             complement(<383..802)
                     /locus_tag="PLV68_20015"
                     /inference="COORDINATES: protein motif:HMM:NF012832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03593.1"
                     /translation="MSAATTSPSSSAPAHRPLVLPARRHLVFVVSGPGGVGKGTIVKA
                     LVERDPQLWLSRSWTTRQQRPGEADDAYVFTGRDAFEARIAADGFLEWTEFLGNYYGT
                     PHPAAVGDRDVVLEIEVDGAQQVKRLDPSTILIFVLPP"
     gene            complement(816..>1149)
                     /gene="mihF"
                     /locus_tag="PLV68_20020"
     CDS             complement(816..>1149)
                     /gene="mihF"
                     /locus_tag="PLV68_20020"
                     /inference="COORDINATES: protein motif:HMM:NF041260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="integration host factor, actinobacterial type"
                     /protein_id="HRE03594.1"
                     /translation="QIGRLAHMATPPALTPEQRANALAKAAEARAARAEFKTKVKLGS
                     VSLAEALGSTDDTVGKLKVVSLLESLPGVGKVKARKVMDDIGIADNRRVKGLGPQQKA
                     ALIDQLGK"
     assembly_gap    1150..1159
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1247..>1869)
                     /locus_tag="PLV68_20025"
     CDS             complement(1247..>1869)
                     /locus_tag="PLV68_20025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HRE03595.1"
                     /translation="ASIWGRSVADYAAAAEQLADAGPDVIAVEVNLSCPNLEGRRGIF
                     AHDAELSAEVIAATAACGRPRWAKLSPNTDRLVEVAGAVAEAGAEAVTLINTVLGMVI
                     DPHTRRPALGNGGGGVSGRAIHPVAVRAVYDVHAALPELAIVGVGGVASGWDAAELLL
                     AGASAVQVGTATFADPRAPWQVLHNLVRWAAAEGIRSLAGLSGEMR"
BASE COUNT          270 a          577 c          579 g          267 t
ORIGIN      
        1 cgagggtgac gaggctgaac ttggagtcga cccggccgag cagttcctcg atcggggggt
       61 tcatcatggt gtcgttgcta cgcgccacag cggcaatctc ctctggtact cgtctgggtg
      121 atcatcaagt tggtgatcct cgccttgaaa gggctgctca agcctaacgt tgtcagccct
      181 ggatcggctc gccggccgcg gccaggcggc aaccgtnnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nngggaggca acacgaagat caggatggtg ctgggatcga
      421 gccgcttcac ctgctgggca ccgtcgactt cgatctcgag cacgacatcg cggtcaccga
      481 ccgcggccgg gtgcggggtg ccgtagtaat tgccgagaaa ctcggtccac tccagaaagc
      541 catcagccgc aatacgcgcc tcgaacgcat cgcggccggt gaacacatag gcgtcgtcgg
      601 cttcgccggg acgctgttgg cgcgttgtcc agctccggct gagccacaac tggggatcgc
      661 gctcgacgag cgccttgacg atcgtgccct tcccgacccc accggggccg gacaccacga
      721 acaccagatg ccgccgggcg ggcagcacga gcggccggtg agcgggcgcg gaagacgacg
      781 gagacgtggt ggcggcgctc acgaacgcag tgcggctact tgccgagctg atcgatgagg
      841 gcggccttct gctgcgggcc gaggcccttg acccggcggt tgtcggcgat gccgatgtcg
      901 tccatgacct tgcgcgcctt gaccttgccc acgccgggca gcgattcgag cagggagacg
      961 accttgagct tgccgaccgt gtcgtcggtg gagccgaggg cttcggccag cgaaacggat
     1021 ccgagcttca ccttggtctt gaattcggcg cgagcagcgc gggcttcggc agccttggcc
     1081 agggcgttgg cgcgctgttc gggggtgagt gcgggaggtg ttgccatgtg ggccagtcta
     1141 ccgatctgan nnnnnnnnnc aggcgaagag gaggttctcc gataaatttg agcgatctga
     1201 ccggatcggc caggtcatca ccccaggtcg cgggcggcgt caggtgtcac cgcatctcgc
     1261 cactgagccc ggccagcgac ctgataccct ccgccgccgc ccagcggacc aggttgtgca
     1321 gcacctgcca cggtgcccgt gggtcggcga aggtcgcggt gcccacctgc acggccgacg
     1381 ccccggccaa cagcagttcg gccgcgtccc atcccgacgc gactcccccg acgccgacga
     1441 tcgccagctc gggcagtgcc gcgtgcacgt cgtacacagc ccgcacggcg acaggatgga
     1501 tcgcccggcc cgacactccc ccgccaccgt tgcccaacgc gggacgccgg gtgtgcgggt
     1561 cgatgaccat gcccagcacg gtgttgatca gcgtcaccgc ttcggccccg gcctcggcga
     1621 ccgcacccgc cacctcgacc aagcggtcgg tgttggggct gagcttggcc caccgcggcc
     1681 gaccgcacgc ggcggtggcc gcgatcacct cggcgctcag ctcggcatcg tgggcaaaga
     1741 tgccacggcg gccttcgagg ttgggacacg acaggttgac ctcgaccgcg atcacgtccg
     1801 gtccggcgtc ggccaactgc tcggctgccg cggcgtagtc ggccaccgaa cgaccccaga
     1861 tgctggcca
//
LOCUS       DAORNF010000691         1865 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_45417_length_1865_cov_1.511602, whole genome shotgun sequence.
ACCESSION   DAORNF010000691 DAORNF010000000
VERSION     DAORNF010000691.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1865
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45417_length_1865_cov_1.511602"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(192..>1039)
                     /locus_tag="PLV68_20030"
     CDS             complement(192..>1039)
                     /locus_tag="PLV68_20030"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: protein motif:HMM:NF008978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HRE03596.1"
                     /translation="ARPKQWAKNVLVFAAPGAAGVLTEATPLLEALVIFVAFCLASAG
                     TYYWNDILDVEADRVHVKKRFRPIASGAVPLGVARVVGVALLAAGIGLSFTTRWQAGV
                     ACIVYIVLTSTYSAIWKHIAVVDLIAVASGFVIRAIAGAAATAVPMSSWFLLVTSFGA
                     LFIVTGKRYAELREHGESPSAARATLNDYSLGFLRIVLSVSCGATLVAYCIWAFEAAD
                     LAGAEWPLYQLSIVPMLAALLRYLLILEQGHGAAPEEIFIADRTLQVLGLVWVVIFGL
                     AVYVN"
     assembly_gap    1040..1139
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1140..>1473)
                     /locus_tag="PLV68_20035"
     CDS             complement(<1140..>1473)
                     /locus_tag="PLV68_20035"
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HRE03597.1"
                     /translation="DETLVGLLRPDTSARLAWHQRQGHQVVLVSASLGAYLRPLAERL
                     GLDGVLCTDGVVGADGRYTGDLRDGNCRGPEKQRRLAEWMFANGVADAPVWAYGDSTG
                     DREMLAAAR"
     assembly_gap    1474..1529
                     /estimated_length=56
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1530..1717)
                     /locus_tag="PLV68_20040"
     CDS             complement(<1530..1717)
                     /locus_tag="PLV68_20040"
                     /inference="COORDINATES: protein motif:HMM:TIGR01488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03598.1"
                     /translation="MTAVAAFDVDGTLTTRDSVVPFLVRVAGRARLAARMLTQPGALG
                     RGIVGRDRDRIKAVAVRAA"
BASE COUNT          306 a          623 c          554 g          226 t
ORIGIN      
        1 cataggcagc aacaagcatg accgagactc tcgccaccct tctttcatgg ctgccgcggc
       61 cgctggtgga tctgctgcgg gcggtgcgac cccagccggt caagcgagtc cgtgtgctca
      121 tggctcgctc agcctaccga cgcgaccaga tcgcgacggg tcgctccaca tggatcagtt
      181 ggcgcgaccg gtcagttgac gtacacggcg aggccgaaga tcaccaccca caccaggccc
      241 aacacctgca gcgtgcggtc ggcgatgaag atctcttctg gggccgcgcc atgcccctgt
      301 tcgaggatca acagatagcg caacagcgcg gcgagcatcg gcacgatcga cagctggtac
      361 agcggccact cggcaccagc caggtcggcg gcctcgaagg cccagatgca ataggccacc
      421 agcgtcgccc cgcagctcac cgacagcacg atccgtagga agcccagcga gtagtcgttg
      481 agcgtggccc gtgccgcgct gggcgattcg ccatgctcgc gcagctcggc gtagcgcttg
      541 ccggtcacga tgaacaacgc accgaacgag gtcaccagga ggaaccaact gctcatcggc
      601 acggcggtgg ccgcagcccc ggcgatggcc cggatgacga accccgacgc gaccgcaatc
      661 agatcgacca ccgcgatgtg cttccagatc gcgctgtagg tgctggtcag cacgatgtag
      721 acgatgcagg ccacaccggc ctgccagcgg gtggtgaacg acaagccgat acccgccgcc
      781 agcagcgcca cgccgacgac acgggccaca ccgagcggca ccgccccact ggcaatcggc
      841 cggaaccgct tcttgacgtg gacgcgatcg gcctcgacgt cgaggatgtc gttccagtag
      901 taggtgcccg cgctggccag acagaacgcg acgaagatca ccagcgcttc cagcagcggc
      961 gtggcctcgg tgagcacacc ggccgctccg ggtgcggcga acacgagcac gttcttggcc
     1021 cactgcttgg gccgcgcctn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc
     1141 gcgccgcggc gagcatctcg cggtctccgg tgctgtcccc atacgcccac actggcgcgt
     1201 cggccacccc gttggcgaac atccactccg ccagacggcg ctgcttttcg ggtccgcggc
     1261 agttgccgtc gcgcaggtcg ccggtgtagc gcccgtcggc gccgacgacc ccgtcggtgc
     1321 acagcacgcc gtcgagtccc aaccgttcgg ccagcgggcg caggtacgcg ccgagcgagg
     1381 ccgacaccag cacgacctga tgaccctgac gctgatgcca cgccaagcga gccgacgtgt
     1441 cggggcggag cagaccgacc agcgtctcgt cgcnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnng cggcacgcac cgcaacggcc ttgatgcgat
     1561 cgcgatcgcg gccgacgatg ccacgtccga gcgcacccgg ctgggtcagc atccgcgccg
     1621 cgagcctggc tcgtccggcg acgcggacca agaacggcac cacgctgtcg cgcgtggtca
     1681 gggttccgtc gacatcgaac gccgcgaccg cggtcatgga gccgccatcg cgtcgagcgc
     1741 ggcccaaaag cccggccagc tcttggagac cacctgtggg tcggcgaccg ccacgcccgg
     1801 caccgccagc cccatcaccg cgaacgccat cgcaaggcga tggtcgtgat gggtgtcgag
     1861 caccg
//
LOCUS       DAORNF010000692         1858 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_45603_length_1858_cov_1.777038, whole genome shotgun sequence.
ACCESSION   DAORNF010000692 DAORNF010000000
VERSION     DAORNF010000692.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1858
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45603_length_1858_cov_1.777038"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="PLV68_20045"
     CDS             complement(<1..315)
                     /locus_tag="PLV68_20045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03599.1"
                     /translation="MRRSYRRADPRWWQQLSDLQVAVLAGSVVLGTWLQRPAAAIALG
                     AICGWRRAWRAVGLAVVVAGVVAGVLSARAWRGTEPDTIGPYRGWACLVTDPAPGHGA
                     VNA"
     gene            314..769
                     /locus_tag="PLV68_20050"
     CDS             314..769
                     /locus_tag="PLV68_20050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03600.1"
                     /translation="MSISPSPGMVYAPRSVVPQGPRAFRYRIRRLGPLLSLLPALVLG
                     VGAVVALQDNTSTTRGVGGFAAAVLAAPGLLAAGIPLTSGTGRLAAGIGASAIAWMVI
                     GAIAARRATRSPVARWRDFWREWMWFALGVWIGVGGALLVAQLALGSLV"
     gene            complement(791..1606)
                     /locus_tag="PLV68_20055"
     CDS             complement(791..1606)
                     /locus_tag="PLV68_20055"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HRE03601.1"
                     /translation="METSPVGRDGESPPSTPSPLRHDPLVRPHDRPPVTRWLLDWLRW
                     FGIARALATVAAVVLVAAGGLWLLRTPPTPVESGLPMASSTSAPSATSPTASTATPPS
                     GSSASPVATEPVSTAPATIVVHVAGAVAQPGIYTLAAGSRVVDAVAVAGGVVADADLD
                     AVNLAGPIGDGTRVYIPRIGGEVPVVVTPSGGGGAGTGAAVSPSPTPSAPIDINAASV
                     AELDALPGVGPSTAAAIVEYRSSVGPFASVEDLLHVRGIGAAKLDAIRDLVRT"
BASE COUNT          252 a          668 c          677 g          261 t
ORIGIN      
        1 cgcgttgacc gctccgtggc caggtgccgg atcggtgacc aggcacgccc acccgcggta
       61 cggcccgatc gtgtccggtt cggtgccgcg ccacgcccgc gctgacagca ccccagcgac
      121 cacacccgcg accacgacgg ccaggcccac ggcccgccaa gcccgccgcc atccgcagat
      181 cgctccgagc gcgatcgcag ccgcgggacg ctgcagccat gtcccgagca ccacgctgcc
      241 ggcgagcacc gccacctgca ggtcggagag ttgctgccac catcgcggat cggcgcgtcg
      301 gtacgatcgc cgcatgtcga tcagcccgag ccctggcatg gtttacgcgc cacgttcggt
      361 cgtgccccag ggaccgcggg cgttccggta tcgcatccgt cgcctcggcc cgttgctgtc
      421 gctgcttccc gccttggtgc tgggcgttgg tgcggtcgtt gccttgcagg acaacaccag
      481 caccacccgc ggcgtcggcg gctttgctgc cgcagtgctg gcggcccctg gcctgttggc
      541 cgcgggcatc ccactgacgt ccggcaccgg tcggctcgcc gctggcatcg gtgccagcgc
      601 gatcgcgtgg atggtgatcg gcgcgatcgc ggcgcggcgg gcaacgcgtt cgcccgtcgc
      661 ccgctggcgc gacttctggc gagagtggat gtggttcgcg ctgggcgtgt ggatcggcgt
      721 gggtggcgcg ctgttggtgg cccagttggc gctgggctcg ctggtctgac gccggcacat
      781 cacggccccg tcacgtccgg accaggtcgc ggatcgcgtc gagcttggcc gcaccgatcc
      841 cgcggacgtg caacaggtcc tcgaccgagg cgaacggacc gaccgacgat cggtactcga
      901 cgatcgcggc ggccgtcgac gggccgaccc cgggcaacgc gtcgagttcc gcgaccgacg
      961 cggcgttgat gtcgatcggc gcgctcggtg tcggactcgg gctcaccgct gctccggtgc
     1021 ctgcgccgcc cccgcctgat ggcgtgacga cgacgggcac ctcgccgccg atgcgcggga
     1081 tgtagacccg ggtaccgtcg ccgatcggac cggccaggtt gacggcgtcg aggtcggcgt
     1141 cggccacgac gccaccagcg acagcgaccg catcgaccac ccgcgacccc gcggccagcg
     1201 tgtagatgcc cggctgggcg acggcgccag cgacgtgaac cacgatcgtc gccggagccg
     1261 tgctcaccgg ttcggtcgca accggcgagg cggatgatcc actcggcggc gtcgccgtcg
     1321 acgcggtcgg tgatgtagcc gacggggccg acgtgctgct ggccatgggc aagccggact
     1381 cgaccggcgt cggtggcgtg cgcagcagcc agagtcctcc ggcggcgacg aggaccaccg
     1441 cagccaccgt ggcaagagcg cgggcgatgc cgaaccaacg caaccagtcg agcagccaac
     1501 gggtgaccgg tgggcggtcg tggggacgga cgagcggatc gtgacgcagg gggctcggcg
     1561 tgctcggcgg cgactcgccg tcgcggccga caggtgacgt ctccatgtgc atctccctcg
     1621 gtggtggcgc acacgcgccg acgacacgtc gggggaagac gcctgcaccg tagcgagtgc
     1681 tggtgccagt tgctgatcgc ggtgggagcg gtctcggcgt cgaccgcggt ggccaacgac
     1741 cggcgggcga cccactcggc ctcgatctcc agtgggatgc gccagtacgc acccttgtgg
     1801 ctcttgccgc ggatgcgcca cagcaggtac cagccgacgt accgggcgag gaacccga
//
LOCUS       DAORNF010000693         1854 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_45730_length_1854_cov_1.472485, whole genome shotgun sequence.
ACCESSION   DAORNF010000693 DAORNF010000000
VERSION     DAORNF010000693.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1854
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45730_length_1854_cov_1.472485"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1039
                     /locus_tag="PLV68_20060"
     CDS             <1..1039
                     /locus_tag="PLV68_20060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HRE03602.1"
                     /translation="RGGLGHLSTLLPPEVITPILREVAGNRQKPPIERMTWHEAMNRF
                     GVDKPDLRFGMELTELTDVFAATEFKAFAGAAAIKGINASGKADEMGRNKLDQMTDRA
                     KKMGAKGLVWLKVNSDRVFESPVAKFLSDAEQAELHQRLATQPGDLVLIVADAWDVTC
                     DVLGTLRNDLGRPPVWQGPYRYVWVTEFPLFVGVDKETGIPKPGHHPFCRPHPDDVDL
                     IDTDPMKVRAMAYDLVLNGWELGSGSVRIHESDLQRKGFHVLGISDDEADRKFGFFLR
                     PFEYGAPPHGGFAFGIDRLVAILAGEENIREIIAFPKTQSGQDPMTNAPTPPAPKQLD
                     ELGLRVLPPKA"
     gene            1058..1498
                     /locus_tag="PLV68_20065"
     CDS             1058..1498
                     /locus_tag="PLV68_20065"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRE03603.1"
                     /translation="MASPHFRAGVVVVVRGIDDRVLAFERGDILGQWQFPQGGLEDGE
                     TPIQAAWRELGEETGLGANDVMAVAEYPGWVTYEYPPDLAVKKGRLGQTQRWFLFDAL
                     RADIRPTPDGREFVAWQWVTREWLIEQTAGFRRAAYELVLSAVT"
     assembly_gap    1508..1552
                     /estimated_length=45
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1556..>1854
                     /locus_tag="PLV68_20070"
     CDS             1556..>1854
                     /locus_tag="PLV68_20070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRE03604.1"
                     /translation="MAQQAPLAARLRPRTLDEVVGQVHLVGPGRPLRRLVEQDRLTSA
                     IFWGPPGTGKTTLALAIAGTTRRAFEQLSAVTAGVKDVREVIERARQRLGEHGRGT"
BASE COUNT          297 a          613 c          631 g          268 t
ORIGIN      
        1 gcgcggcggg ctggggcatt tgtcaacgct gctgccgccg gaggttatca ccccgatatt
       61 gcgcgaagtg gcgggcaatc gccagaagcc gccgatcgag cggatgacct ggcacgaggc
      121 gatgaaccgc ttcggtgtcg acaagcctga cctgcgcttc ggcatggagc tgaccgagct
      181 gaccgacgtg ttcgccgcga ccgagttcaa ggcgttcgcc ggtgcagccg cgatcaaggg
      241 catcaacgcc tcgggcaagg ccgacgagat gggccgcaac aagctcgacc agatgaccga
      301 ccgcgccaag aagatgggcg ccaagggcct ggtgtggttg aaggtcaaca gcgatcgggt
      361 cttcgagtct cccgtcgcga agttcctcag cgacgccgaa caggccgaac tgcaccagcg
      421 cctcgccacc cagccgggcg acctcgtact gatcgtggcc gacgcgtggg acgtgacctg
      481 cgacgtgctc ggcacgctac gcaacgacct cggccgtcca ccggtgtggc aggggccgta
      541 ccgctacgtg tgggtcaccg agttcccgct gttcgtcggc gtcgacaagg agaccggcat
      601 ccccaagccc ggccaccacc cgttctgccg cccccacccc gacgacgtcg atctgatcga
      661 caccgacccg atgaaggtgc gggcgatggc ctacgacttg gtgctcaacg ggtgggagct
      721 cggctcgggc agcgtccgta tccacgagag cgacctgcag cgcaaggggt tccacgtgct
      781 gggtatcagc gacgacgagg ccgaccgcaa gttcggcttc ttcctccgcc cgttcgagta
      841 cggcgccccg ccccacggcg ggttcgcgtt cggcatcgac cggttggtgg cgatcctggc
      901 tggtgaggag aacatccgcg agatcatcgc cttccccaag acccagagcg gtcaggaccc
      961 gatgaccaac gcgccgacgc cacccgcgcc caagcagctc gacgagctgg gcctgcgcgt
     1021 gctgccgccc aaagcctgac ctgctacaac agcccgcatg gcgagtcctc acttccgagc
     1081 cggtgtggtc gtcgtggtgc gcggcatcga cgatcgtgtg ctcgccttcg aacgtggcga
     1141 cattctgggc cagtggcagt tcccccaggg tggcctggag gacggggaga ccccgatcca
     1201 ggcggcgtgg cgtgaactgg gtgaagagac cgggctcggc gcgaacgacg tgatggccgt
     1261 ggccgagtat cccggctggg tcacctacga gtacccgccc gatctggcgg tcaagaaggg
     1321 ccgcctcggc caaacccagc ggtggttcct gttcgacgcg ctgcgggccg acattcgccc
     1381 cacgcccgac ggtcgcgagt tcgtcgcttg gcagtgggtg acccgtgagt ggctgatcga
     1441 acagaccgcg ggcttccgcc gcgcggcgta cgaactcgtg ctgtcggcag tcacctgagc
     1501 actgcggnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncggctggc
     1561 ccagcaggca ccgctcgcgg cgcggctgcg gccgcgcaca ctcgacgagg tggtcggcca
     1621 agtacacctg gtgggaccgg ggcgtccgct gcgccgactg gtcgagcagg accggctgac
     1681 ctcggcgatc ttctgggggc cacccggcac gggtaagacc acgctcgcgt tggcgatcgc
     1741 cggcacgacc cgccgcgcgt tcgaacagct ctcggcggtg accgcggggg tcaaggacgt
     1801 gcgtgaggtc atcgagcgcg cccggcaacg gctgggcgag cacgggcggg gcac
//
LOCUS       DAORNF010000694         1854 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_45733_length_1854_cov_1.423013, whole genome shotgun sequence.
ACCESSION   DAORNF010000694 DAORNF010000000
VERSION     DAORNF010000694.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1854
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45733_length_1854_cov_1.423013"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="PLV68_20075"
     CDS             complement(<1..199)
                     /locus_tag="PLV68_20075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03605.1"
                     /translation="MTAVPASLDPLDPRYRDEAAVRGELTRTFQVCNGCRKCVTLCAA
                     FPSLFRMLDRIGDGDAGRLTPD"
     gene            279..>858
                     /locus_tag="PLV68_20080"
     CDS             279..>858
                     /locus_tag="PLV68_20080"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRE03606.1"
                     /translation="MTASPASSGDPTFDRARALQRLATEHFDVVVIGGGITGVGVALD
                     AVTRGLTVALVERDDFASGTSSKSSKLIHGGLRYLQQGEVALVYEALRERQRLRKNAP
                     HLVEVLPFMIPILTRDGVVSRKIARVMGTSMWMYDLTGGWRIGKLHRRLRRPKAVAHF
                     PTMRAERLASAYLYFDARADDARLCLTVARTAA"
     assembly_gap    859..883
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <884..>1854
                     /locus_tag="PLV68_20085"
     CDS             <884..>1854
                     /locus_tag="PLV68_20085"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRE03607.1"
                     /translation="VTAVHTQPGQPSVVTVDAGEPGSTESIPVENRNIESRNIESRHI
                     EIRADVVVNATGVWTDDVRALASGEHPDSIRPAKGVHITVPWHTVGNDIAVIIPVPKD
                     RRSLFVIPWEPLPDGTFRHAYVGTTDTDYQGDVDDPRCTADDVAYVLAALNASVTTAI
                     TEADITGVWAGLRPLVKSAPDGRTADLSRRHSITVDDSGVISVAGGKLTTYRDMAEDT
                     VDRVLKQLDRRARCVTAKLGLLGAEGYREPAPEQRRLAHRYGTLRVELDALVATDPSL
                     AEPLVPGLGYIRAEAVYAVRNEMALTLDDVLSRRTRARLADRDAAVA"
BASE COUNT          288 a          683 c          612 g          246 t
ORIGIN      
        1 ggtcgggagt gagtcgcccg gcgtcgccgt cgccgatgcg atcgagcatg cggaacaggc
       61 tcgggaacgc agcacagagg gtgacgcact tgcggcaccc gttgcagacc tggaacgtgc
      121 gggtgagctc gccgcgcacc gcagcctcgt cgcgatagcg cgggtcgagc gggtcgagcg
      181 aggcaggtac ggcggtcatc agcaccgccg acggtagccg acggcctgcg gtcatccgga
      241 ggcgatccgg aaatcccccg ccaagtagat tcccaggcgt gacggcctca cccgccagca
      301 gcggcgatcc caccttcgac cgtgctcgag cgctgcaacg gctggccacc gagcacttcg
      361 acgtggtggt gatcggcggt ggcatcaccg gcgtgggcgt cgccctcgat gcggtcacgc
      421 gcggcctcac cgtcgcgctg gtcgagcgcg acgacttcgc cagtggcacg tcgtcgaaga
      481 gctcgaagct gatccacggc ggcctgcgct acctgcaaca gggcgaggtg gcactggtct
      541 acgaagcgct gcgcgagcgc cagcggctgc gcaagaacgc accccacctg gtggaggtgc
      601 tgccgttcat gatcccgatc ctcacccgcg acggcgtggt atctcgcaag atcgcccggg
      661 tgatggggac gtcgatgtgg atgtacgacc tgaccggcgg atggcgcatc ggcaagctgc
      721 accgccgctt gcgccgcccc aaggccgtcg cccacttccc caccatgcgt gccgagcggc
      781 tcgcgtcggc ctacctctac ttcgacgctc gcgccgacga cgcccggctg tgcctgaccg
      841 tggcccgcac cgcggcccnn nnnnnnnnnn nnnnnnnnnn nnncgtcacc gcggtgcaca
      901 cccaacccgg ccagccctcg gtcgtgaccg tcgatgccgg cgaacctggc tcgaccgaga
      961 gcatccccgt cgagaaccgc aacatcgaga gccgcaacat cgaaagccgg cacatcgaga
     1021 ttcgggccga cgtggttgtc aacgccaccg gggtgtggac cgacgacgtg cgcgccctgg
     1081 ccagcggcga gcaccccgac tcgatccgcc ccgccaaggg cgtgcacatc accgtgccct
     1141 ggcacacggt gggcaacgac atcgccgtca tcatcccggt accgaaagac cggcgcagcc
     1201 tgttcgtcat cccgtgggag ccgttgcccg acggcacgtt ccgccacgcc tacgtcggca
     1261 ccaccgacac cgactaccag ggcgacgtcg acgatccccg gtgcaccgcc gacgacgtcg
     1321 cctacgtgct ggccgcgctc aacgcctcgg tcaccaccgc gatcaccgag gccgacatca
     1381 ccggcgtgtg ggccgggttg cggccgctgg tgaagtcggc cccggatggg cgcaccgccg
     1441 acctgtcccg tcgccacagc atcaccgtcg acgactccgg cgtgatcagc gtggcgggcg
     1501 gcaagttgac cacctatcgc gacatggccg aggacacggt cgatcgggtg ctgaagcagc
     1561 tcgatcgccg cgctcggtgc gtcaccgcca agctgggctt gctcggcgcc gagggatacc
     1621 gcgaaccggc gcccgaacag cggcggctcg cccaccgcta cggcacgctg cgggtcgagc
     1681 tcgacgccct ggtcgccacc gacccgagcc tcgccgagcc cctcgtgccc ggcctcggct
     1741 acatccgcgc cgaggcggtg tacgccgtgc gcaacgagat ggcgctgacg ctcgacgacg
     1801 tgctgtcgcg ccgcacccgg gctcgtctgg ccgaccgcga cgcggccgtc gccg
//
LOCUS       DAORNF010000695         1847 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_45954_length_1847_cov_1.294643, whole genome shotgun sequence.
ACCESSION   DAORNF010000695 DAORNF010000000
VERSION     DAORNF010000695.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45954_length_1847_cov_1.294643"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1256
                     /locus_tag="PLV68_20090"
     CDS             <1..1256
                     /locus_tag="PLV68_20090"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HRE03608.1"
                     /translation="ILPFLEAIRQFRKEVGRDNVCYIHVTLVPFIGPSGEQKTKPTQH
                     SVTELRSRGILPDVIVCRSESPLSDNLKYKISNLRDVEERAVVNAADARNIYELPLIL
                     HGEGLDDVVCDVLRIDAEPDLSAWERLVATIDAAKRPVTIGLIGKYVDLQDAYLSVAE
                     SLRHAGFHHGAKVDIDWIQAEDVEGLLAAGRLADLDGMVIPGGFGERGFEGKIAAAAY
                     AREHRVPCLGICLGLQAMTVDFARHVLQLERANSSEMDPTTPHPVIDLMHDQRDVLDK
                     GGTQRLGAFYAILEPDTIVHRAYGEPVVSERHRHRYELNAHLKPRLEAAGLRCSGLSP
                     DRRLVEFVELDDHPFWVATQAHPEFKSRPDRAHPLFRELVAAALVRREARGAAGNGTA
                     AAVNGSRATAPSPAPAGEPASEPVG"
     gene            1256..>1484
                     /locus_tag="PLV68_20095"
     CDS             1256..>1484
                     /locus_tag="PLV68_20095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03609.1"
                     /translation="MAAGAGFRHRSDRLVHQGHIWHVAVGSFTDPGGNEFERDIVRSP
                     GAVGVVPLLFDAEGNPSVVMIRQYRPPYDEYV"
     assembly_gap    1485..1529
                     /estimated_length=45
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1542..>1847
                     /locus_tag="PLV68_20100"
     CDS             1542..>1847
                     /locus_tag="PLV68_20100"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HRE03610.1"
                     /translation="MTAARELTEEVGLRAAQLQHLTDFYPSAGMTDSVLYLYLATGLT
                     EVDHERHGPEEEYMEVLHLPLGDALAMIERGEIKDAKSVIGLLLTDRMLTRGDVAVVS
                     "
BASE COUNT          297 a          612 c          610 g          283 t
ORIGIN      
        1 agattttgcc gtttctcgaa gcgatccgcc agttccgcaa ggaggtcggc cgcgacaacg
       61 tctgctacat ccacgtcacc ctggtgccgt tcatcggtcc gagcggcgag cagaagacca
      121 agccgacgca gcacagcgtc accgagctgc gcagccgagg cattctgccc gacgtcatcg
      181 tgtgccggag tgagtcaccg ctcagcgaca acctgaagta caagatcagc aatctgcgtg
      241 acgtcgagga gcgggccgtg gtcaacgccg ccgatgcgcg caacatctac gaactgccgc
      301 tgatcttgca cggtgaagga ctcgacgacg tcgtctgcga cgtgctgcgg atcgacgccg
      361 agcccgatct cagcgcgtgg gagcggttgg tcgccaccat cgatgccgcg aagcgccccg
      421 tcacgatcgg tctgatcggc aagtatgtcg atctgcagga cgcctacctg tccgtggccg
      481 agagtctgcg ccacgccggc tttcaccacg gagccaaggt cgacatcgac tggatccagg
      541 ccgaagacgt cgagggcttg ttggctgccg gacgtctggc cgacctcgac ggcatggtga
      601 ttcccggcgg cttcggtgag cgcggcttcg agggcaagat cgccgccgcg gcctacgccc
      661 gcgagcaccg ggtgccatgc ctaggcatct gcctcggcct gcaggcgatg accgtcgact
      721 tcgcgcgtca cgtgttgcag ctggagcggg ccaactcgtc ggagatggac ccgaccacgc
      781 cgcacccggt gatcgacctg atgcacgacc agcgcgacgt gctcgacaag ggtggcacgc
      841 agcgccttgg cgcgttctac gcgatcctcg aacccgacac catcgtccac cgtgcctacg
      901 gcgaaccggt ggtcagcgag cgccaccgtc accgctacga gctcaacgcg cacctgaagc
      961 cgcgcctcga ggcggccggc ctgcggtgca gcgggctgtc gcccgaccgt cgcctggtcg
     1021 agttcgtcga gctcgacgac cacccgttct gggtcgcgac tcaggcccac cccgagttca
     1081 agagccgccc cgatcgcgct catccgttgt tccgcgagct ggtcgcggca gcgctcgtgc
     1141 gccgtgaggc gcgcggcgcg gctggcaacg ggaccgcggc tgcggtcaac gggagccggg
     1201 ccacggcccc ctccccggca cccgctggcg agccggccag cgaacctgtc ggctgatggc
     1261 cgccggcgcg gggttccggc accgatcgga ccgcctggtg caccaggggc acatctggca
     1321 cgtggccgtg ggcagcttca ccgatccggg gggcaacgag ttcgaacgtg acatcgtccg
     1381 ctcgcccggc gcagtcggcg tggtgccgct gctgttcgat gccgagggca acccgtctgt
     1441 ggtgatgatt cgccagtacc ggccgccgta cgacgaatac gtctnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnng aaccgaccga ggtgaccgcg gcgcgtgagc
     1561 tgaccgaaga ggtcgggctg cgggccgcgc aactccagca cctgaccgac ttctatccgt
     1621 cggccgggat gaccgactcg gtgctgtatc tgtatctcgc cacgggtctg accgaggtcg
     1681 accacgagcg ccacggaccg gaggaggagt acatggaggt gctccacctg ccgttgggcg
     1741 atgcgttggc gatgatcgag cggggcgaga tcaaagacgc caagagcgtg atcgggctgt
     1801 tgctgaccga ccggatgctc acccgcggcg atgtcgcggt cgtcagt
//
LOCUS       DAORNF010000696         1846 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_45969_length_1846_cov_1.706868, whole genome shotgun sequence.
ACCESSION   DAORNF010000696 DAORNF010000000
VERSION     DAORNF010000696.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45969_length_1846_cov_1.706868"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>235
                     /locus_tag="PLV68_20105"
     CDS             <1..>235
                     /locus_tag="PLV68_20105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HRE03611.1"
                     /translation="SEDALSPYVEGGFDHATWGADVTLTTLNLCRETLDGVRNHSWSR
                     PAPMTPEGEVVSWADRIAYVCHDFEDAVAAGIVT"
     assembly_gap    236..332
                     /estimated_length=97
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <333..>629
                     /locus_tag="PLV68_20110"
     CDS             <333..>629
                     /locus_tag="PLV68_20110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HRE03612.1"
                     /translation="MVEPIAEALAAFRAFNYESVYLRPASTAQASAVIAMLRALVEHY
                     ADRPNLLARTDDGYAPDQSARGVLHGVGAASPEAYAEAVRYVGGMTDRFACQQAV"
     assembly_gap    630..746
                     /estimated_length=117
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(915..>1094)
                     /locus_tag="PLV68_20115"
     CDS             complement(915..>1094)
                     /locus_tag="PLV68_20115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002513859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HRE03613.1"
                     /translation="GGSTVTAGSILIRQRGTRFHPGTNVGLAKDDSLFALTDGVVEFG
                     ARRGRRTINIVPTAE"
     assembly_gap    1095..1104
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1105..1182)
                     /locus_tag="PLV68_20120"
     CDS             complement(<1105..1182)
                     /locus_tag="PLV68_20120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007369914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HRE03614.1"
                     /translation="MSKTKGGGSTKNGRDSNSQRLGVKAF"
     gene            complement(1186..1497)
                     /gene="rplU"
                     /locus_tag="PLV68_20125"
     CDS             complement(1186..1497)
                     /gene="rplU"
                     /locus_tag="PLV68_20125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HRE03615.1"
                     /translation="MYAVIATGGKQERVAEGQQVRVELLGAEDGADVTFTPILVVDGD
                     AVLATPAELQGASVTAKVLSTVKGVKVNGFTYKRRTNQRKRWGHRQKYSLVEITSIAK
                     G"
     gene            complement(1550..>1846)
                     /locus_tag="PLV68_20130"
     CDS             complement(1550..>1846)
                     /locus_tag="PLV68_20130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HRE03616.1"
                     /translation="NLEAAEEVAKQLRLRDIGGIIVIDFIDMEIRENRRKVIDAFKQA
                     LARDKTRTQVFDISDLGLVEMTRKRIGEGLLTNFADPCPACEGRGVVVDLARLE"
BASE COUNT          239 a          554 c          550 g          279 t
ORIGIN      
        1 ccagcgagga cgcgctgagc ccctacgtcg agggcgggtt cgaccacgcc acctggggcg
       61 ccgacgtcac cctgaccacg ctcaacctgt gtcgggagac gctcgacggg gttcgcaacc
      121 actcctggtc gcgcccagcg ccgatgaccc ccgagggcga ggtcgtctcg tgggccgacc
      181 gcatcgccta tgtctgccac gacttcgaag atgccgtcgc agcgggcatc gtcacnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnatggtcga gccgatcgcc gaggcgctgg
      361 ccgcgttccg ggcgttcaac tacgagtcgg tctacctccg gcctgcctcg acagcccaag
      421 ccagcgccgt catcgcgatg ctgcgcgccc tggtcgagca ctacgccgac cgccccaacc
      481 tgttggcccg caccgacgac ggctacgccc ccgaccagtc ggcgcgcggt gtgctccatg
      541 gtgtcggcgc ggccagcccg gaggcatacg ccgaggcggt ccgctacgtc ggcggtatga
      601 ccgaccgctt cgcctgccag caggccgtcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnnnnaaat tggccacccc ggcgatttcg acgtccgcgg
      781 agcggcgatt gcccggaaac cggcaactct gcactccgcg gcagccggat tgaccacccc
      841 ggtccatctg gctcggccgg aaaacggcga cggccccggg ggttgcccgg ggccgtgcgt
      901 cgaactgaga tcggttattc ggcggtggga acgatgttga tcgtgcggcg accgcggcgg
      961 gcgccgaact cgaccacgcc gtcggtcagg gcgaacaggc tgtcgtcctt ggccagaccg
     1021 acgttggtgc cggggtgaaa gcgggtgccg cgctggcgga tcaagatgct gcccgcggtg
     1081 accgtggagc cgccnnnnnn nnnngaaggc cttgacgccg aggcgctggg agttggagtc
     1141 gcggccgttc ttggttgaac cgccaccctt tgtcttcgac atgattcagc cctttgcgat
     1201 cgaggtgatc tccacgaggg agtacttctg acggtgtccc cagcgcttgc gctggttggt
     1261 gcggcgcttg taggtgaagc cgttgacctt gacgcccttg acggtgctga gcaccttggc
     1321 ggtcaccgag gcgccctgca gttcggccgg ggtggccagc actgcatcgc cgtcgacgac
     1381 caggataggg gtgaaggtga cgtcggcgcc gtcttcggcc ccgagcagct cgacccgcac
     1441 ctgctggcct tcggcgactc gttcctgctt accgccggtt gcgattactg cgtacatagc
     1501 ggggcgatcc tatcggtgtg ccggggtgat ccaaacgcct tcgcggcact cactcgaggc
     1561 gggccagatc gaccaccacg ccgcggccct cgcacgcagg gcacgggtcg gcgaagttgg
     1621 tcagcagccc ctcaccgatg cgcttgcgtg tcatctcgac caggccgagg tcggagatgt
     1681 cgaacacctg ggtacgggtc ttgtcgcggg cgagggcctg cttgaaggcg tcgatcacct
     1741 tgcgccggtt ctcgcggatc tccatgtcga tgaagtcgat cacgatgatg ccgccgatgt
     1801 cgcgcagccg caactgcttc gcgacctcct cggccgcctc gaggtt
//
LOCUS       DAORNF010000697         1842 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_46094_length_1842_cov_1.396195, whole genome shotgun sequence.
ACCESSION   DAORNF010000697 DAORNF010000000
VERSION     DAORNF010000697.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46094_length_1842_cov_1.396195"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="PLV68_20135"
     CDS             <1..471
                     /locus_tag="PLV68_20135"
                     /inference="COORDINATES: protein motif:HMM:NF019428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HRE03617.1"
                     /translation="VRALRDACSSQVHLPGVRPVRDRGSVAVEMVLLTPVLVVMMVFV
                     VFLGRAGGASQQVRHAADAAARAASMVSPARMQAAAHATATADLSANGVNCASTSVSV
                     SLTAGAESGQVAVSSVTVTVHCTTDSQGLGLLRVGARTLTAMSTELVDEHRGGA"
     gene            537..>867
                     /locus_tag="PLV68_20140"
     CDS             537..>867
                     /locus_tag="PLV68_20140"
                     /inference="COORDINATES: protein motif:HMM:NF024792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HRE03618.1"
                     /translation="MVITIAVIACAGLVFDGGRLVSAKISAADHADNAARAGAQAVSV
                     GSGGPLIDPLEAHRRAQAYLSSAGVSGTVVATPTGVSVTVTAPVPMTLLAVAGVGTKT
                     VSAPRSAE"
     assembly_gap    868..1019
                     /estimated_length=152
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1020..>1460
                     /locus_tag="PLV68_20145"
     CDS             <1020..>1460
                     /locus_tag="PLV68_20145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03619.1"
                     /translation="KNGDISDTVVIKALAVLVWIAWVRLAISTIVELVARGRGANAPR
                     VVGLGATQRWAAGLIAAIVLMAAAPQSALAGSADPSRPIRAALLQADPVRAPMAAPAS
                     YEFAIDPLLAAAPRAVRAERPTSATVREHVVVRHDSFWSIAEDEL"
     assembly_gap    1461..1593
                     /estimated_length=133
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          199 a          508 c          579 g          271 t
ORIGIN      
        1 gtgcgggcac tcagggatgc gtgttcgtct caggtccatc tgcccggcgt ccgtccggtg
       61 cgcgatcggg gcagcgtcgc ggtcgagatg gtgctgctca cccccgtgct ggtggtgatg
      121 atggtgttcg tcgtgttcct cggtcgggcg ggtggtgctt cgcagcaggt ccgtcatgcg
      181 gccgatgcgg ccgctcgcgc cgcgtcgatg gtgtcgcccg cgcggatgca ggccgcggcc
      241 catgccacgg ccaccgccga cctcagcgcg aacggtgtca actgtgcctc gacgtcggtg
      301 tccgtctcgc tgacggcagg tgcggagtcg ggacaggttg ctgtcagcag cgtcacggtc
      361 acggtgcact gcacgaccga ctcgcagggc cttggcttgt tgcgagtggg agcgcgcaca
      421 ctcaccgcga tgtcgaccga gttggtcgac gaacaccgcg gtggagcgtg atggcgactg
      481 cgcggcgcag tgatcatgac tgtgttcgcg atcgtggcag cgtcagcggc ttcgtagtgg
      541 tgatcacgat tgccgtcatc gcctgcgcgg gtttggtatt cgacggcggg cggttggtca
      601 gtgccaagat cagcgcggcc gaccacgccg acaatgctgc tcgtgccggt gcccaggcgg
      661 tgtcggtcgg cagcggaggc ccattgatcg atccgctcga agcgcatcgc cgcgcccagg
      721 cctatctgag ctcggccggt gtcagtggca cggtcgtcgc gaccccgacc ggcgtgtcgg
      781 tcaccgtgac cgcgccggtg ccgatgacgc tgctcgcggt tgcgggggtc ggcaccaaga
      841 ccgtgtccgc gccgcgatct gccgaacnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc
     1021 aagaacggag acatctccga cactgtcgtg atcaaggcgc tggccgtctt ggtgtggatc
     1081 gcatgggtgc gtctggcgat cagcacgatc gtcgagttgg tcgcccgtgg tcgtggggcg
     1141 aacgcgccgc gcgtcgtcgg gctcggtgcc acgcagcggt gggcggccgg gttgatcgcc
     1201 gcgatcgtgt tgatggctgc ggcgccgcag agcgcactcg cggggtcggc cgatccgtcg
     1261 cggccgattc gcgccgcgct cttgcaagcc gatccggtgc gtgcaccgat ggccgcccca
     1321 gcaagctacg agttcgcgat cgacccgctg ctcgcagccg cgccgagagc ggttcgcgcc
     1381 gagcgaccga cgtcggcgac ggtgcgcgag cacgtcgtcg tgcgtcacga ttcgttctgg
     1441 tcgatcgccg aagacgaact nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncccgatg ccggcgttgc tgggacgagt
     1621 gtggagccgg tctccccgtc cggtgcagtg gtggcgtcgg cgcccagttc gacggtcgca
     1681 cccggagtgg gttcgccgtc ggccccggtg tccgactggt ccgagccagc acccggcgcg
     1741 acgccagacg ccgtggccga gcctgggatc ggaccgacga ccgtcgcggt cgaacgaggc
     1801 gacacgctca gctcgatcgc cgccgacgtg ttgggcgacg cc
//
LOCUS       DAORNF010000698         1841 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_46109_length_1841_cov_2.020717, whole genome shotgun sequence.
ACCESSION   DAORNF010000698 DAORNF010000000
VERSION     DAORNF010000698.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1841
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46109_length_1841_cov_2.020717"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="PLV68_20150"
     CDS             complement(<1..398)
                     /locus_tag="PLV68_20150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03620.1"
                     /translation="MSRTFAELVNRRRQMFAESGSPHEWDALFHGLVPPSEQLLAFVG
                     AEESKLFRAKHYLLGITEQSLIVVPTNGSGTKRTGDPIVLAAGEITVTEADHNTFNFT
                     ARGAEYSLVSSEGEAGHYEFDFEAEGVDDST"
     gene            complement(499..1107)
                     /locus_tag="PLV68_20155"
     CDS             complement(499..1107)
                     /locus_tag="PLV68_20155"
                     /inference="COORDINATES: protein motif:HMM:NF024248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRE03621.1"
                     /translation="MVDDWEPTGPTTSAELVLTDLDVLGEIVHPMRSRILRHLGQPHT
                     AADLAALLDMPVTRLYHHLNRLEQVGLIHVVATRRVAAVTERRYQVVAQRFLLGRDLG
                     PDIEPAEVALAIGSVFDFTKLQLQREIERAPRDRALPDLRTRSAISMSEYRVAPGRAQ
                     EFIDRVRALIDEFEHDYADGEPPETDPADRVALLIAAFPVTD"
     gene            1189..>1841
                     /locus_tag="PLV68_20160"
     CDS             1189..>1841
                     /locus_tag="PLV68_20160"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRE03622.1"
                     /translation="MSEIGIGHVESAGAPRSALGRRFMTVWAGQTVSAIGSNIAGIGM
                     AVFVFLETGSTIWLGVLTAAAAAPYALLGPVLHLVDRWPRKAVMIGGDCVAAVGTAFA
                     LTMAGIGQLEIWHLAVGALLGGIGSAFQTPAAQAAVPLLVPPSELGRANGLAQLGPAV
                     GIVSGPLLAAPLVAWWGITAVLVVDLVTFVVAMATVVAVRIDEGAAIRADIIGSGASV
                     "
BASE COUNT          285 a          546 c          677 g          333 t
ORIGIN      
        1 gtggagtcgt cgacgccttc ggcttcgaag tcgaactcgt agtggccggc ctctccttcg
       61 gatgagacca gcgagtactc ggccccgcgc gctgtgaagt tgaaggtgtt gtggtcggct
      121 tcggtaacgg tgatctcacc cgctgcgagc acgatcggat caccggtgcg cttggtgccg
      181 ctgccgttgg tgggcacgac gatcaggctc tgctcggtga tgcccaacag gtagtgcttg
      241 gcgcggaaca gcttcgactc ctccgccccg acgaacgcca acagttgctc gctcggcgga
      301 acgaggccgt ggaacaacgc gtcccattcg tgcggcgaac ccgactcggc gaacatctgc
      361 cgacggcggt tgaccaattc tgcgaaggtc ctgctcatgg ggcaacggta gtcaacgcac
      421 gcggactacc aggacgagtg ggtcggctgg cggcgcgtcg aaggcgtgag tcatctcgac
      481 aggatcccag ccgaaggctc agtcggtgac ggggaaggcg gcgatcaaga gcgcgacccg
      541 atcggcgggg tcggtctcgg ggggttcgcc gtcggcgtag tcgtgttcga actcgtcgat
      601 cagggcgcgg acgcggtcga tgaactcctg tgcccgtccg ggcgcaactc ggtactcgga
      661 catggagatc gcggatctcg tgcgaaggtc ggggagtgct cggtcgcgcg gggcgcgctc
      721 gatctcgcgc tgcaactgga gcttggtgaa gtcgaacacc gaaccgatgg caagcgcaac
      781 ctcggcaggc tcgatgtcgg ggccaaggtc tcgtcccagc aggaaccgtt gggcgacgac
      841 ctgatagcgg cgctcggtca cggcggcgac ccgccgggtc gcgacgacgt ggatcaggcc
      901 gacctgctcg agccggttca ggtggtggta cagccgggtg acgggcatgt cgagtagcgc
      961 ggccaggtcg gcggcggtgt gcggttggcc gaggtggcgc aggatgcgac tgcgcatcgg
     1021 atggacgatc tcgccgagga cgtcgaggtc ggtgagcacc agttcggccg acgtggtcgg
     1081 cccggtcggt tcccagtcat caaccatggg tcgatcgtat cgttcatgcc caatgaacaa
     1141 atgttcaatt atcttgaaca atatggttca agatgattga ataacgcgat gagtgagatc
     1201 ggtatcggtc acgtcgaatc tgccggcgcg ccgcggtcag cgctgggacg tcgtttcatg
     1261 actgtgtggg ccggccagac ggtgagcgcg atcggttcga acatcgctgg catcggtatg
     1321 gcagtgttcg tgttcctcga gaccggcagc acgatctggc tcggcgtgct cacggccgcg
     1381 gccgccgcgc cctacgcact gctgggaccg gtgctccatc ttgtcgaccg gtggccgcgc
     1441 aaggcggtga tgatcggcgg cgactgcgtg gccgccgtcg ggaccgcctt cgcgctgacg
     1501 atggccggca tcgggcagct cgagatctgg catctcgcgg tgggagccct gctgggtggc
     1561 atcggtagcg cgttccagac gcctgcggcc caggcggccg tgccgctgtt ggtgccgccg
     1621 agcgagctcg gtcgagccaa cggactggcc cagctcggcc cggcggtagg gatcgtctcc
     1681 gggccgttgc tggcggcccc actcgttgcg tggtggggga tcacggcggt actggtggtc
     1741 gacctggtga ccttcgtggt ggcgatggcg acggtcgtcg ccgtgcggat cgacgagggc
     1801 gcggccatcc gcgccgacat aataggctcg ggtgcgtccg t
//
LOCUS       DAORNF010000699         1840 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_46153_length_1840_cov_1.549580, whole genome shotgun sequence.
ACCESSION   DAORNF010000699 DAORNF010000000
VERSION     DAORNF010000699.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1840
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46153_length_1840_cov_1.549580"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..539)
                     /locus_tag="PLV68_20165"
     CDS             complement(<1..539)
                     /locus_tag="PLV68_20165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HRE03623.1"
                     /translation="MSGFDAIELEVLWQSLIATVNEQARALQRAAFSPIVREAGDLAN
                     AVFDRRGRMVAQAVTGTPGHINSLARAASAILDEYPVDTLVEGDVLVTNDPYKTAGQL
                     LDVTVLYPVFRDGRVIAFFGSTIHHTDVGGYGIGAGGRDVFEEGLWIPIVRLMIAGER
                     NHDVWKFILSNVRQPDHMAG"
     gene            complement(536..1150)
                     /locus_tag="PLV68_20170"
     CDS             complement(536..1150)
                     /locus_tag="PLV68_20170"
                     /inference="COORDINATES: protein motif:HMM:NF039581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03624.1"
                     /translation="MVRPIATVAVAERDALLDELRDEGRRVLAAAGVAASNIRFRYGV
                     DARYIGQGNEITIWVGEGEQWPATSDEVVAQFEQDYRRIYGLSIPDVGVEAVTWRLSA
                     YAEANPVEPMAQLSDAAGAVHTTRPVRFVRGAAAIDTPVYRRVDLGVGQTIEGPAIVE
                     ERETTAVIRPGWTATVAPDGSLVALRDAASSNRAATTSNRKADQ"
     gene            complement(1226..1827)
                     /locus_tag="PLV68_20175"
                     /pseudo
     CDS             complement(1226..1827)
                     /locus_tag="PLV68_20175"
                     /inference="COORDINATES: protein motif:HMM:NF014071.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
BASE COUNT          293 a          644 c          629 g          274 t
ORIGIN      
        1 cccgccatgt ggtcgggctg gcggacgttg gagaggatga acttccacac gtcgtggttg
       61 cgctcgccgg cgatcatcag ccgcacgatc ggaatccaca gaccctcttc gaacacgtcg
      121 cggccacctg caccgatgcc gtaaccgccg acgtcggtgt ggtggatcgt gctgccgaag
      181 aacgcgatca cgcgcccgtc gcggaacacg gggtacagca ccgtgacgtc gagcagctga
      241 ccggccgtct tgtacgggtc gttggtgacc agcacgtcgc cttcgaccaa ggtgtcgacc
      301 gggtactcgt ccaagatcgc cgacgccgct cgggcgaggg agttgatgtg gccgggcgta
      361 ccggtgacgg cctgggccac cattcggccg cgacggtcga acaccgcgtt ggccaggtcg
      421 ccggcttcac gcacgatcgg cgagaacgcc gcccgctgca gcgcccgcgc ctgctcgttg
      481 accgtggcga tcaggctctg ccacagcact tccagttcga tcgcatcgaa gccgctcact
      541 ggtctgcctt cctgttgctg gttgtggccg ccctgttgct ggatgccgcg tcgcgcaacg
      601 cgaccagcga gccgtcgggt gcgaccgtgg cggtccaacc cggacggatg acggcggtgg
      661 tctcgcgctc ttcgacgatc gcaggcccct cgatggtctg gccgaccccg agatcgaccc
      721 ggcggtacac cggcgtgtcg atcgccgcgg caccacggac gaaccgcact ggccgggtgg
      781 tgtgcacggc gccggccgcg tcggacagct gcgccatcgg ctcgaccggg ttggcctcgg
      841 cgtaggccga caggcgccac gtcacggctt cgacaccgac gtcggggatc gacaagccgt
      901 agatacggcg gtagtcctgt tcgaactggg cgaccacttc gtcggaggtc gcgggccact
      961 gctcgccttc gccgacccag atcgtgatct cgttgccctg gccgatatag cgggcatcga
     1021 cgccgtagcg gaagcggatg ttgctcgccg cgacaccggc cgcagccagc actcggcgac
     1081 cttcgtcgcg gagctcgtcc agcagcgcgt cgcgctcggc aacggcgacc gtggcgatcg
     1141 ggcgcaccat gctgcgggcg aggtcgatgc gcaccggggt gaccagcgtg ccgaacgcgg
     1201 acagcacgct ggcgttgacc gggaagatca ccgacggcga ctccagcagt tcggctacgc
     1261 cgcaggcgtg caccggaccg gcaccgccga acgcgatcat cggcacgccg cgcaggtcga
     1321 cgcccatctc caccgcgtgc atgcgtgacg aggcggccat gttctggttg acgagctcgt
     1381 gaattccgcc agcggtctct tcgggcgaca gaccgaggcc cgcggcgacc gaaccgatcg
     1441 cggcgttcat cgccgcagtg tcgagggcca tctcgccgcc gaggaagaac gacgcgtcga
     1501 gcatgcccaa cgacaggtgc cgccacggcc gtaacacgcc gggccggggt cggcaccggc
     1561 cgactcgggg ccgaccttca acagaccgag ttcgtcgaca cgggcctggc tgccgccacc
     1621 ggcaccgatc tcgaccaggt cgaccgacgg caccgacacc gggtacccgg agcccttctt
     1681 gaagcggtag atccgggcga cttcgaaggt gttggtcagc tcgggctcgc cgtcgacgat
     1741 gaggcacgac ttggccgtgg tgccacccat gtcgaagcac agcagacggt cttcgccgag
     1801 gcggcgggcg aaccagcttc cggccaacgc acccgcggcc
//
LOCUS       DAORNF010000700         1839 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_46193_length_1839_cov_1.233744, whole genome shotgun sequence.
ACCESSION   DAORNF010000700 DAORNF010000000
VERSION     DAORNF010000700.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1839
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46193_length_1839_cov_1.233744"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>279)
                     /locus_tag="PLV68_20180"
     CDS             complement(<1..>279)
                     /locus_tag="PLV68_20180"
                     /inference="COORDINATES: protein motif:HMM:NF019758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03625.1"
                     /translation="HESDLLVAESIRRGLFDGLGPAATAALMSVFVYEHRSPDPPPLP
                     WFPNADVRRRWHGIAAISAELQSIEERDGLAVHRPPDPTFLAVAYAWAA"
     assembly_gap    280..409
                     /estimated_length=130
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<410..>657)
                     /locus_tag="PLV68_20185"
     CDS             complement(<410..>657)
                     /locus_tag="PLV68_20185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03626.1"
                     /translation="VDPPKALGQIDLPIDLLGDRAKYLAEATQRLERATLTGKPRRAL
                     VAVSDDGAPHPVEDDPDLKHRLRAAAQAERIAREIDDI"
     assembly_gap    658..731
                     /estimated_length=74
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<732..>1451)
                     /locus_tag="PLV68_20190"
     CDS             complement(<732..>1451)
                     /locus_tag="PLV68_20190"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HRE03627.1"
                     /translation="QFIAQLEAGIAAHHAGMVPPFKEVVEACFVAGLIKVVFATETLA
                     VGINMPARTVVIEKLTKFTGEHHTMLTPGEYTQLTGRAGRRGIDERGQAIVLWNPFTT
                     FEQVAHLASSRWFHLNSAFRPTYNMAANLVRTYTSDQAHHLLNLSFAQYQADGDVVRL
                     ESRLGRRQAQLRALADEAVSPYGDIDDYRARIAAADDERRRANAPVGGNDPIDLAMMK
                     LRPGDVIYVDAAAVVRWQRVIA"
     assembly_gap    1452..1594
                     /estimated_length=143
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1595..>1839)
                     /locus_tag="PLV68_20195"
     CDS             complement(<1595..>1839)
                     /locus_tag="PLV68_20195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03628.1"
                     /translation="QLENLFMVGDKTADRVHLMPTLVNGRPNNAAIRLVDQGERAWRG
                     NRGRDKNTTARRKLYTPSRLDVVELLDDEGMLPAIYFV"
BASE COUNT          200 a          487 c          548 g          257 t
ORIGIN      
        1 cgccgcccac gcgtaggcga cggcgagaaa cgtcgggtcg ggcgggcgat gtacggccag
       61 cccgtcgcgc tcttcgatcg actgcagctc cgcgctgatg gccgcgatgc cgtgccaacg
      121 gcgacgaacg tcggcgttgg ggaaccacgg cagcggtggc ggatcagggc tgcggtgctc
      181 gtagacgaac accgacatga gtgcggcggt cgccgccggg ccgaggccgt cgaacaaccc
      241 gcgccggatc gactcggcga ccagcagatc gctctcgtgn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng gatgtcgtcg
      421 atctcgcgag cgatccgctc ggcttgggcc gcggcccgca ggcggtgctt caggtcgggg
      481 tcgtcttcca ccgggtgcgg cgcgccgtcg tcgctcactg ccaccagggc gcggcgcggc
      541 ttgccggtga gcgttgctcg ttccagccgc tgagttgcct ccgccaggta cttggcgcgg
      601 tcgccgagca ggtcgatggg caggtcgatc tggcccagcg ccttgggtgg gtcgacgnnn
      661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      721 nnnnnnnnnn nggcgatcac ccgctgccag cgaacgacgg ccgcggcatc gacgtagatc
      781 acgtcgccgg ggcgcagctt catcatcgcc aggtcgatcg ggtcgttgcc ccctaccggc
      841 gcgttggcgc ggcggcgttc gtcgtcggcc gcggcgatgc gggcccggta gtcgtcgatg
      901 tcgccgtacg ggctgacggc ctcgtcggcc aaggcgcgca gttgcgcttg acgtcgcccg
      961 agacggctct ccaggcggac cacgtcgccg tcggcctggt actgggcgaa gctgaggttg
     1021 agcaggtggt gagcctggtc gctggtgtag gtgcggacca ggttggcggc catgttgtag
     1081 gtgggccgga acgccgagtt gagatggaac cagcggctcg acgccagatg ggcgacctgc
     1141 tcgaaggtgg tgaacgggtt ccacagcacg atcgcctggc cccgctcgtc gatgccacgc
     1201 cgccccgccc gcccggtgag ctgggtgtac tcccccggcg tcagcatggt gtggtgctcg
     1261 ccggtgaact tggtgagctt ctcgatcacc accgtgcgcg ctggcatgtt gatgcccacc
     1321 gcgagcgtct cggtggcgaa caccaccttg atcagcccgg ccacgaagca ggcctcgacc
     1381 acctccttga acggtggcac catgccggca tggtgggcgg cgatgcccgc ttcgagttgg
     1441 gcgatgaact gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnacgaaa tagatcgcgg gcagcatccc
     1621 ttcgtcgtcc aacagctcca ccacgtcgag ccggctgggc gtgtacagct tgcgtcgcgc
     1681 ggtggtgttc ttgtcgcggc cgcggttgcc gcgccatgct cgctcgcctt ggtcgaccaa
     1741 gcggatcgcg gcgttgttgg ggcgtccgtt gaccagcgtc ggcatcaggt ggacgcggtc
     1801 ggcggtcttg tcgccgacca tgaacaggtt ctcgagctg
//
LOCUS       DAORNF010000701         1835 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_46279_length_1835_cov_2.306180, whole genome shotgun sequence.
ACCESSION   DAORNF010000701 DAORNF010000000
VERSION     DAORNF010000701.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1835
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46279_length_1835_cov_2.306180"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1516)
                     /locus_tag="PLV68_20200"
     CDS             complement(<1..>1516)
                     /locus_tag="PLV68_20200"
                     /inference="COORDINATES: protein motif:HMM:NF014615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HRE03629.1"
                     /translation="ESYLAAGADIISTNTFSSTTVAQREYGLDAAQVVHELNVAGARL
                     AREAADAATLTTGRQRFVAGSIGPTNVTLSLSPRVEDPGYRALDFAQLATAYGQQIAA
                     LVEGGADLLLVETIFDTLNAKAAIWAARRYSAQMGFDVPLMISGTITDRSGRTLSGQT
                     VGAFWQSVRHADPVSVGLNCALGAADMRPHVQELSRLADTMICVYPNAGLPNALGGYD
                     ETPDDMSGVLGEFATAGFLNIVGGCCGTTPDHIRAIAAAVAEAPPRRPADAPKLLQLS
                     GLESFTLTDDIPFVNVGERTNVTGSAKFRRLVKNGEFAAALDVARDQVENGAQVIDVN
                     MDEGLLDSREAMVTFLRLIASEPDIARVPVMIDSSKFSVIEAGLECVQGKSIVNSISL
                     KEGEEAFLATARVCRDHGAAVVVMAFDEKGQADSVERKVAICSRAYDLLVDKLDFPPQ
                     DIIFDPNIFAIATGIAEHDRYGLDFIEATEILRRRFPLVNISGGVSNLSFSFRGN"
     assembly_gap    1517..1623
                     /estimated_length=107
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          281 a          542 c          626 g          279 t
ORIGIN      
        1 cgttgccgcg gaacgagaac gacaggttgg acacgccgcc ggagatgttg accaacggga
       61 agcgccggcg gaggatctcg gtggcttcga tgaagtcgag gccgtagcgg tcgtgctcgg
      121 cgatgccggt ggcaatggcg aagatgttgg gatcgaagat gatgtcctgc ggcgggaagt
      181 cgagcttgtc gacgagcagg tcgtaagccc gcgagcagat cgccacctta cgctcgaccg
      241 aatcggcctg gcccttctcg tcgaaggcca tcaccaccac cgccgcgccg tgatcgcggc
      301 acacgcgggc agtggccagg aacgcctcct cgccttcctt gagcgagatc gagttgacga
      361 tcgacttgcc ctgcacgcat tcgagaccgg cctcgatcac gctgaacttg gacgagtcga
      421 tcatcaccgg cacgcgggcg atgtcgggct cgctggcgat caggcgcaag aacgtcacca
      481 ttgcctcgcg cgagtcgagc aggccttcgt ccatgttgac gtcgatcacc tgcgcgccgt
      541 tctcgacctg atcgcgggcg acgtcgagcg cggcggcgaa ctcgccgttc ttgaccagac
      601 gacggaactt ggccgaaccg gtgacgttgg tgcgctcgcc cacgttgacg aacgggatgt
      661 cgtcggtgag tgtgaacgac tccagtccgg acagttgcaa cagcttcggc gcgtcggccg
      721 gccggcgcgg tggagcctcg gccacggccg cggcgatcgc gcggatgtga tcgggggtcg
      781 tgccgcagca gccgccgacg atgttgagga atcccgcggt ggcgaactcg ccgaggacgc
      841 ccgacatgtc gtcgggtgtc tcgtcgtagc cgcccagcgc gttggggagc ccggcgttgg
      901 gatagacgca gatcatcgtg tcggccagcc gcgacagctc ctgcacgtgg ggacgcatgt
      961 cggccgcgcc gagcgcgcag ttgagcccga ccgacacggg gtcggcgtgg cgcacggact
     1021 gccagaacgc gccgacggtc tggcccgaca acgtacgtcc cgaacggtcg gtgatcgtgc
     1081 ccgagatcat cagcggcacg tcgaagccca tctgcgccga atagcggcgg gccgcccaga
     1141 tggctgcctt ggcgttgagc gtgtcgaaga tggtctcgac cagcaacagg tcggcgccgc
     1201 cttcgaccaa cgccgcgatc tgctggccgt aagccgttgc cagttgggcg aagtcgaggg
     1261 cccgatagcc ggggtcttcg acccgcggcg agagcgacag cgtgacgttg gtcggcccga
     1321 tcgaacccgc cacgaaccgc tggcggcctg tggtcagggt ggccgcgtcg gccgcctcgc
     1381 gggccaggcg ggcaccggcc acgttcagct cgtggacgac ctgggcggca tcgaggccgt
     1441 attcgcgctg ggcgacggtg gtcgacgaga acgtgttggt cgagatgatg tcggccccgg
     1501 cggccaggta cgactcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnngaggccg tggcgctgaa tctcggtacc cgcggcacca tcgaggacga ggatccgttc
     1681 cgcagcggca cggcgaagcg cctctcgggc aactgaggca tgaggcaagt cgggcacggt
     1741 gcggttcctt cctacggcga gacgcgtgcc aacgggttgg gcgtcggttc gccgatcttg
     1801 tgcagcgtgt ggttgtcggt gtgagcgaat ggttc
//
LOCUS       DAORNF010000702         1827 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_46550_length_1827_cov_1.826749, whole genome shotgun sequence.
ACCESSION   DAORNF010000702 DAORNF010000000
VERSION     DAORNF010000702.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46550_length_1827_cov_1.826749"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(62..>1013)
                     /locus_tag="PLV68_20205"
     CDS             complement(62..>1013)
                     /locus_tag="PLV68_20205"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HRE03630.1"
                     /translation="TSPHIVAIEVVLPDGAVTILGGEEAEPDGYDLRGAFVGSEGMFG
                     IATRICVRLTQNPPTVRTMLLDFASVAAGAATVSAIIADGMVPAALEMMDALCTQAVE
                     AYIHAGLPTDAAAILLVEVAGLEHRLADEVERIVAIGTMHGARSVRVAQDEAERALLW
                     KGRKTAFGAIARIKPNYYLHDTVVPRRRLPEVLAQVYEIAARHDLLVMNVFHAGDGNL
                     HPLLVFDKREPGVMPRVHAAGHEIVAASVAAGGVLSGEHGIGLEKRDYMPMMFGPLDL
                     AAQASMRAAFDPTGLANPTKVLPSPSSCGDAHGVAEGAWI"
     assembly_gap    1014..1023
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1024..1416)
                     /locus_tag="PLV68_20210"
     CDS             complement(<1024..1416)
                     /locus_tag="PLV68_20210"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRE03631.1"
                     /translation="MPLSHIATLEYGFDGQAGVVCFPLTTAEVQAVVQACCRHEQPWV
                     ARGSGTGLAGGATPLDGAVVISLAKMNRIVSVDAGARTAWVEPGVLNLDLSRAVHHLG
                     LHFAPDPSSQQSCTIGGNVANNSGGPHCL"
     gene            complement(1516..>1827)
                     /locus_tag="PLV68_20215"
     CDS             complement(1516..>1827)
                     /locus_tag="PLV68_20215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03632.1"
                     /translation="AAQLAPVNVAKSAAAKVRNAGSEVVEAVREGRRAMRDKEAELHA
                     RMHSVAAPLADELAAGGAGLAPDDQILVDGRPVQPGQVIVLRQVRDQQRRSARRARRG
                     A"
BASE COUNT          255 a          660 c          633 g          269 t
ORIGIN      
        1 cctgcaccgg cgacggtgac cggaccggcc gaatcgtcac cgaccgcggc ggcgaacgcg
       61 gtcagatcca cgcgccctcc gccacgccat gggcgtcgcc gcacgacgac gggctgggca
      121 gcaccttggt cgggttggcc aacccggtgg ggtcgaacgc ggcccgcatc gacgcctgcg
      181 cagccaggtc gagtgggccg aacatcatcg gcatgtagtc gcgcttttcc agcccgatgc
      241 cgtgctcacc gctgagcacg ccacccgctg ccaccgacgc ggcgacgatc tcgtgccctg
      301 ccgcgtgcac ccgcggcatc acgcccggct cacgcttgtc gaacaccagc aacgggtgca
      361 ggttgccgtc gccggcgtgg aacacgttca tcaccagcag gtcgtggcgg gccgcgatct
      421 cgtacacctg cgccaacacc tcgggcaggc gccgccgggg caccacggtg tcgtgtaggt
      481 agtagttcgg cttgatccgg gcgatcgcac cgaaggccgt cttgcggccc ttccacaaca
      541 gcgcccgttc ggcttcgtcc tgggcgacgc gcaccgagcg cgccccgtgc atcgttccga
      601 ttgcaacgat ccgctcgacc tcgtcggcga ggcgatgttc cagaccggcg acttcgacca
      661 gcaggatcgc tgccgcgtcc gtcggcagcc cggcgtggat gtatgcctcg accgcttggg
      721 tgcacagcgc atccatcatc tccagtgcgg cgggcaccat gccgtcggcg atgatcgcgc
      781 tgaccgtcgc tgcgccggcg gccaccgagg cgaagtcgag cagcatcgtg cgcaccgtcg
      841 gtgggttctg ggtcaggcgc acgcagatgc gggtggcgat gccgaacatg ccctcgctgc
      901 cgacgaacgc accgcgcagg tcgtatccgt cgggctccgc ctcctcgccg ccgaggatgg
      961 tgaccgcgcc gtcgggcaac accacttcga tggcgacgat gtgcggcgag gtgnnnnnnn
     1021 nnncagacag tgcgggcccc ccgagttgtt ggccacgttg ccgccgatgg tgcagctctg
     1081 ctggctgctc ggatcgggcg cgaagtgcag gccgaggtgg tgcacggcgc ggctgaggtc
     1141 gaggttgagc accccgggtt cgacccacgc ggtgcgcgcc ccggcgtcga ccgacacgat
     1201 gcggttcatc ttcgccagcg agatcacgac cgcaccgtcg agcggggtcg ctcccccggc
     1261 caggccggtc cccgacccac gggccaccca cggctgctcg tggcggcagc acgcttgcac
     1321 cacggcttgc acctcggccg tggtcagcgg aaagcaaacg acaccggcct ggccgtcgaa
     1381 gccgtactcg agcgtggcga tgtgcgaaag cggcacgctg tggccggacg cggtcggcac
     1441 cgagcaggcc gcgcagatca ctggcgatcg cggcgagccg cttgggtgct gacgctgtcg
     1501 gtgccgctgt tgtggtcatg cgccgcgacg ggcacgccgc gcagatcggc gctgctggtc
     1561 gcgcacctgg cgcagcacga tcacctgccc aggctgcacc gggcggccgt cgaccaggat
     1621 ctgatcgtcg ggtgccagac cggcaccgcc ggccgcaagt tcgtcggcga gcggcgcggc
     1681 cacgctgtgc atccgcgcgt gcagttcggc ctccttgtcg cgcatcgccc gacggccctc
     1741 gcgcacggcc tcgaccacct cgctgccggc gttgcggact ttggccgcgg ccgacttggc
     1801 gacgttgacc ggcgccagtt gagcggc
//
LOCUS       DAORNF010000703         1826 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_46575_length_1826_cov_1.996612, whole genome shotgun sequence.
ACCESSION   DAORNF010000703 DAORNF010000000
VERSION     DAORNF010000703.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46575_length_1826_cov_1.996612"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="PLV68_20220"
     CDS             <1..232
                     /locus_tag="PLV68_20220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03633.1"
                     /translation="PDHLAACAGIDLDPDGTLYYRYVLASRLHTTIDPDLLIELIAMV
                     EFHQEHYGDFLEGVTDGGIAIEVLDAVIRHAG"
     gene            300..>1826
                     /locus_tag="PLV68_20225"
     CDS             300..>1826
                     /locus_tag="PLV68_20225"
                     /inference="COORDINATES: protein motif:HMM:NF015150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase family protein"
                     /protein_id="HRE03634.1"
                     /translation="MNASSTVDVVDFQAPDAPIRFTRSLHETGFAVLVNHPLPVELVQ
                     SIYDEWLAFFDTEAKEAYRFSDDNQDGYFAPEVSETAKGNDKRDIKEFFHVYPWGRYP
                     TEVSEGALRYAEQAKAIAQTLLGWVDDNTPPEVAARFSRPLRNMMNDSTRSLLRILRY
                     PPLRGDEEPGAVRAAAHEDINLLTVLPASNEAGLQVQDTAGNWHDVPCDFGSLAINCG
                     DMLDLASGGYYPSTTHRVTNPTGEAATRSRLSLPLFLHPADDVVLAEGRTAFSFLQER
                     LRELRSQDLKAKPGRCQRPALTPPRRAHDTPTTMASATVRSIDGALIRRGRADLIARD
                     DLSGRTLARRLSDQMDGWIDALAHDLPADWSVIATGGYATGLLSPGSDIDVVLLHPPK
                     TPADTVRAVAESIWYPMWDSGVKLAPAAHSQKSLLSLAADDLHTATTLLRVRPLAGSA
                     EQAAAVRTAAREQWRKRSHHWLTELRAASKVRWAKAGDVAALLEPDLKDGAGGLRDYD
                     T"
BASE COUNT          306 a          665 c          589 g          266 t
ORIGIN      
        1 cccggatcac cttgcggcct gcgccggtat cgacctcgac cccgacggca cgctctacta
       61 ccgctacgtg ctcgcgtccc ggctgcacac cacgatcgat cccgacctgt tgatcgaatt
      121 gatcgcgatg gtcgagttcc accaagagca ctacggcgac ttcctcgaag gcgtcaccga
      181 tggcggcatc gcgatcgaag tgctcgacgc cgtgatccgc cacgccggct gacccgagcg
      241 ccgaccacgg ccgggcgccc aacgatttcg gggatggcat ggggcggtag cgtgctgcta
      301 tgaacgcctc ttcgacggtc gacgtggtcg acttccaggc tcccgacgct ccgatccgct
      361 tcacccgctc gttgcacgag accgggtttg ccgtgctggt caaccatccg ttgccggtcg
      421 aactggtgca gagcatctac gacgagtggc tggccttctt cgacaccgaa gccaaggagg
      481 cgtaccgctt cagcgacgac aaccaggacg gctacttcgc acccgaggtg tcggagacgg
      541 ccaagggcaa cgacaagcga gacatcaagg agttcttcca cgtctacccc tggggccgct
      601 accccaccga ggtcagcgag ggtgcgctgc gctacgccga gcaggccaag gcgatcgccc
      661 agacgctgct cggctgggtc gacgacaaca ccccgcccga ggtcgcggcc cggttcagcc
      721 gcccgctgcg caacatgatg aacgacagca cccgcagcct gttgcgcatc ctgcgctacc
      781 ccccgctgcg cggcgacgaa gagccgggcg ccgtgcgcgc cgcggcccac gaggacatca
      841 acctgttgac ggtgctcccc gcatccaacg aggccgggct ccaggtgcag gacaccgctg
      901 gcaactggca cgacgtgccg tgcgacttcg gcagcctggc gatcaactgc ggcgacatgc
      961 tcgatctggc cagcggcggc tactacccct cgaccaccca ccgggtcacc aaccccaccg
     1021 gcgaggcggc cacgcggtcg cggctcagcc ttcccctgtt cctgcatccc gccgacgacg
     1081 tggtgctggc cgagggacgc acggcgttct cgttcttgca ggagcggttg cgggaactac
     1141 gcagccaaga cctcaaggcc aagccggggc gctgccagcg acccgcgctc acgcccccac
     1201 ggcgagctca cgacaccccc acgacgatgg catcggcaac ggttcgctcg atcgacggcg
     1261 ccctgatccg gcgcgggcga gccgacctga tcgctcgcga cgacctgtcg ggacgcaccc
     1321 tggcccggcg cctgtccgat cagatggacg gctggatcga cgcgctcgcc cacgacctgc
     1381 ccgccgactg gtcggtgatc gccaccggcg gttacgcaac agggttgctc agcccgggca
     1441 gcgatatcga cgtcgtgctg ctgcaccccc cgaagacccc ggccgacacc gtgcgcgccg
     1501 tggccgagtc gatctggtac ccgatgtggg actccggtgt gaagctggca cctgctgcgc
     1561 actctcagaa gtcgttgctg tcgttggccg cagatgacct gcacaccgcg accacgctgt
     1621 tgcgggtacg gcccctggcc ggatcggccg agcaggccgc ggcggtgcgc accgcggccc
     1681 gtgagcaatg gcgcaagcgc agccaccact ggttgaccga actgcgcgcg gcgagcaagg
     1741 tgcggtgggc caaggcgggc gacgtcgccg cgctgctcga acccgatctg aaggacgggg
     1801 caggtggtct gcgtgactac gacacg
//
LOCUS       DAORNF010000704         1823 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_46694_length_1823_cov_1.644231, whole genome shotgun sequence.
ACCESSION   DAORNF010000704 DAORNF010000000
VERSION     DAORNF010000704.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46694_length_1823_cov_1.644231"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            408..>1385
                     /locus_tag="PLV68_20230"
     CDS             408..>1385
                     /locus_tag="PLV68_20230"
                     /inference="COORDINATES: protein motif:HMM:NF012503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HRE03635.1"
                     /translation="MSDSHSPRYRAVVSDLRQVFPQPVSDRVHDAYLVFSFLRALDQL
                     DAMKSVSPILGEPVQLDYEAAKSRRVADNPQSLEDVTSELVAHLAGMFIWGHPRSQIN
                     VVPPPTIASVIGGLLPSLYNPNLVSEESAKRVAVAEVEVSAMVAELVGYDSATSAGLF
                     TFGGSGTLLYGAKLGLQKCCPNEGRQGRTERAVLFCSQQAHYACLSVANWLGIGQDNV
                     VQIPCGDDNQMHIDELETAIRREVAAGARIAAIVATMGTTDHFGLDDLHAVVELRDRL
                     VDELSLDYVPHVHADAVIDHRQLEQATLSALDEAIAEAHRATTRGVKSIG"
     assembly_gap    1386..1395
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1396..>1823
                     /locus_tag="PLV68_20235"
     CDS             <1396..>1823
                     /locus_tag="PLV68_20235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03636.1"
                     /translation="GTHRRIQFLKLADSIGIDFHKTGFTPYISSMFLSRDAHDLDLIA
                     RGADETPYLFHDGHYHPGRYTLETSRSGGGPMAALANLLLFGKNGLRSLLGHLVSMAE
                     SLREHLDGHAATTVVNRENFGPVTLFRIYPDGVDTFDVLRR"
BASE COUNT          320 a          695 c          466 g          332 t
ORIGIN      
        1 ccttgccgaa agacgacccc aagcagcgct gccctgacat cacccgcgcc cgtcagattc
       61 tcggctggga acccaaattc cccctcgccg aaggcctcaa atccaccgtc gcctactacc
      121 gcgaagccct cggcatcaag gcctgatcgc agcggccgaa cacgatctca aagcgcttca
      181 acccaattgg ccgcgaccca ccggggagcg ctgcttcgcg atctctccgt gccttggcgg
      241 ctccgtgaga aacccgtctt catcttcact cacggacatc cacacctctg tcgactctag
      301 cccctcgtct ctcgactcta gaccctcgac tctagacaat actctccgtg accgacaccc
      361 ccactcggtc gtccaaccac cgacatccct gccgcaggat tcccaccgtg tccgattcgc
      421 actcgccgcg ctaccgggcc gtcgtgtccg atttgcggca agtctttccg cagcccgtct
      481 cggaccgtgt tcacgacgcc tacctggtct tctccttcct ccgcgccctc gaccagctcg
      541 atgccatgaa gtcggtctcc ccgattctcg gggaaccggt ccagctcgat tacgaggcgg
      601 ccaagtcgcg acgcgtggct gacaacccac agtcgctcga ggatgtcacg tccgaactcg
      661 ttgcccacct cgccggcatg ttcatctggg gacacccgcg ctcgcagatc aatgtcgtcc
      721 cgccccccac catcgccagc gtcatcggcg gcttgctccc gtcgctctac aatcccaacc
      781 tcgtcagcga ggaatccgcc aaacgcgtcg ccgtcgccga ggtcgaggtc tctgcgatgg
      841 tcgccgaact cgtcggctac gactctgcca cgtccgccgg cctcttcacc ttcggcggct
      901 cgggaactct gttgtacggc gcgaaactcg gtctccagaa atgctgcccc aacgagggtc
      961 gccagggacg aactgagcga gccgtcctct tctgctccca gcaggcccac tatgcctgcc
     1021 tcagcgtcgc caactggctc ggcatcggcc aggacaacgt cgtgcagatt ccctgcggcg
     1081 acgacaatca gatgcacatc gacgaacttg agactgccat ccgccgcgaa gtcgccgccg
     1141 gtgcccgcat cgcggccatc gtcgcgacca tgggcacgac cgatcatttc ggcctggatg
     1201 acctgcacgc ggtcgtcgaa ctccgcgatc gtctcgtgga cgaactgtcg ctcgattacg
     1261 tcccccacgt ccatgccgac gccgtcatcg accaccgtca gctcgaacaa gccaccctga
     1321 gcgccctgga cgaggcgata gctgaggctc accgcgccac cactcgtggc gtaaagagca
     1381 ttggtnnnnn nnnnnggcac gcatcgccgc attcaattcc tgaagctcgc cgactcgatc
     1441 ggcatcgact tccacaagac cgggttcacg ccctacatct ccagcatgtt cctctcccgc
     1501 gatgcgcacg atctcgatct catcgcccgc ggagccgacg aaacccccta tctctttcac
     1561 gacggccact atcatcccgg gcgctatacc ctggaaacct cgcgcagcgg cggcggcccg
     1621 atggcggcgc ttgccaatct gctgctgttc ggcaagaacg ggctgcggtc gctgctcggc
     1681 cacctggtct caatggccga gtcgctccgc gaacatctcg acggccacgc cgccaccacg
     1741 gtcgtcaatc gcgagaactt cggacccgtc acgctgttcc gcatctatcc cgacggggtc
     1801 gacacgttcg atgtgctgcg ccg
//
LOCUS       DAORNF010000705         1820 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_46788_length_1820_cov_1.659490, whole genome shotgun sequence.
ACCESSION   DAORNF010000705 DAORNF010000000
VERSION     DAORNF010000705.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1820
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46788_length_1820_cov_1.659490"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..678)
                     /locus_tag="PLV68_20240"
     CDS             complement(<1..678)
                     /locus_tag="PLV68_20240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03637.1"
                     /translation="MRPIPLGRRVAAATAVVALATASACGSDTAGPSTEPVTSVVPTS
                     SAPSATTTVPNTTTAAPTTTTPGTTTTPGTTGGTAASTTSVPATTTQPAITSTSAATT
                     TTAATAKYTPVASPVPPRQTAPVSAGGTQADGVYYASVATGDPPPTPAAIAFDLLQLF
                     VGQACIEHFGDDDPDACVGDYGIEIVPTALVLATLDGVFISVVDAATKQSYQVSGAEL
                     AELLIGGE"
     gene            complement(757..>1540)
                     /gene="coaA"
                     /locus_tag="PLV68_20245"
     CDS             complement(757..>1540)
                     /gene="coaA"
                     /locus_tag="PLV68_20245"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HRE03638.1"
                     /translation="TVYLSLTRLLNLYVSATQNLQKVTSTFLGTLAPRVPYIIGVAGS
                     VAVGKSTFARILRALLARWPDHPRVSLITTDGFLYPNQTLEHRGLMTRKGFPESYDTR
                     RLISFLRDVKSGAAEVRAPIYSHVVYDIVEGEETVVTQPDILIVEGLNVLQVGSDAGE
                     FVSDYFDFSIYVDADEADIESWYVARFQALRETVFKDPNSYFQHYSHLTEEQAVHVAH
                     GIWRDINGRNLVENIAPTRERASLVLRKGPDHRITKVMLRRL"
     assembly_gap    1541..1550
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1551..1698)
                     /locus_tag="PLV68_20250"
     CDS             complement(<1551..1698)
                     /locus_tag="PLV68_20250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03639.1"
                     /translation="MQLGTESGRYLSFTRDEWALLRAATPLTLRQEHLDELRGINERI
                     DLDEV"
BASE COUNT          251 a          544 c          684 g          331 t
ORIGIN      
        1 ctcgccgccg atcaacagct cggcgagctc ggcgcccgag acctgatagc tctgcttggt
       61 ggcggcgtcg accacggaga tgaacacgcc gtcgagggtc gccagcacca gggctgtcgg
      121 cacgatctcg atgccgtagt cgccgacaca ggcgtcaggg tcgtcgtcgc cgaagtgctc
      181 gatgcaggcc tggccgacga acagttgcag aaggtcgaag gcgatcgccg ctggcgtcgg
      241 tggcggatcg ccggtggcga ccgaggcgta atacacgccg tcggcttgtg ttccaccagc
      301 gctgaccggt gccgtctggc gcgggggcac gggcgaggcg acgggcgtgt atttggcggt
      361 cgcggccgtg gtggttgtcg ctgcgctcgt actggtgatg gctggttgcg tcgtggtcgc
      421 cggcacggat gtcgtactgg ctgcggtgcc gccggtggtg ccgggggtgg tcgtcgtgcc
      481 cggcgtggtc gtggttggtg ctgcggtggt cgtgttgggc acggtggtcg tcgcgctcgg
      541 tgccgaactg gtcgggacga cggacgtgac gggctcggtc gatggtccgg cggtgtcgct
      601 tccacacgcg ctggccgtgg cgagcgcgac gacggcggtg gccgccgcga cgcggcgacc
      661 gagggggatg ggacgcattc tggtcaatct aggtccgcgc cacgccgagc gcccgaacga
      721 cccgccgttc gggatgtcgt gagagggcca gacgtgtcag aggcgacgca gcatcacctt
      781 ggtgatgcgg tggtctggtc ctttgcgcag caccagcgag gcgcgctcgc gggtcggagc
      841 gatgttctcg accaggttgc ggccgttgat gtcgcgccag atgccgtggg cgacgtggac
      901 cgcctgctcc tcggtgaggt ggctgtagtg ctggaagtag ctgttcgggt ctttgaacac
      961 ggtctcgcgc agcgcctgga agcgggcgac gtaccaggac tcgatgtcgg cctcgtcggc
     1021 atcgacgtag atgctgaagt cgaagtagtc gctgacgaac tcaccggcgt cgctgccgac
     1081 ctgcaacacg ttgagccctt cgacgatcag gatgtcgggt tgggtcacga ccgtctcctc
     1141 gccctcgacg atgtcgtaga cgacgtggct gtagatcggc gcccgcacct cggccgcgcc
     1201 gctcttgacg tcgcgcaaga agctgatcag ccgccgcgtg tcgtagctct cggggaagcc
     1261 tttgcgggtc atcaaccctc ggtgctccaa cgtctggttg gggtacaaga acccgtcggt
     1321 ggtgatcagg ctgacgcgcg gatggtcggg ccagcgggcc agcagggcgc gcaggatgcg
     1381 ggcgaaggtg ctcttgccga ccgccacgct gccggccaca ccgatgatgt acggcacgcg
     1441 tggcgcgagt gtgccgagga acgtcgacgt caccttctgc aggttctggg tggcactgac
     1501 gtacaggttg agcaatcggg tcaacgacag gtacaccgtg nnnnnnnnnn cgacctcgtc
     1561 gaggtcgatc cgttcgttga taccgcgcaa ctcgtcgagg tgctcttggc gcagcgtcag
     1621 cggggtggct gctcgcaaca gcgcccactc gtcgcgcgtg aagctgaggt agcgccccga
     1681 ttcggttccc agttgcacga gtgctgaccc tactgtcgcg cccgcccggc tcccaagggc
     1741 ggccgggcgt cggccccgtg ggacgagcgg tcgtgggtat ggtctggccg tgggagccgg
     1801 tctcgttggt ggtagtgccg
//
LOCUS       DAORNF010000706         1819 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_46822_length_1819_cov_1.498299, whole genome shotgun sequence.
ACCESSION   DAORNF010000706 DAORNF010000000
VERSION     DAORNF010000706.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46822_length_1819_cov_1.498299"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1503)
                     /locus_tag="PLV68_20255"
     CDS             complement(<1..1503)
                     /locus_tag="PLV68_20255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HRE03640.1"
                     /translation="MSTPPAGPATSAPSEPPRSPEPPGALTIDSREPVRFLGREFEYK
                     WVVTAVLVSALFLDILDTTIVNVALPTLVSEFGESATEWTVIGYTLSLAVFIPVSGWL
                     SDRMGNKRIFMFAFGMFIVASALCALSQSVGQLIAFRVLQGVGGGMLTPVGMAMLFRV
                     FPPAERARVAVVMMVPAMAAPALGPIIGGVLVTHLSWRWIFTINIPIGLLALAIGGTL
                     LHEHREPTAGNFDLPGFLLSAIGFAGFVYGVSDGPRSGWTSPVVLITGLGGLAVIALM
                     VVVELRRSTPMLQLRLLQDRLFRQTNLVSVMTTGSFMGIMFVIPLYLQSLRGMSAQES
                     GLTTFPQAIGVAVASVIAGRVYLRIGPRRLIAAGLAGAAIAIGAFAFITLGTSLWVIR
                     GLMFARGFCMGFAFVPMQAASYATIAPADNGRAASIFSTQRQVAVSVAVAVFATVLAT
                     YVSYGAHGVIVNPDRALTGFHVTFMIAAGMAIVGSLVALMIKDSDAAGTMA"
BASE COUNT          337 a          626 c          619 g          237 t
ORIGIN      
        1 ggccatggtg ccggccgcat cggaatcttt gatcatcagc gcgacgagtg agccgacgat
       61 ggccatgccg gccgcgatca tgaacgtgac gtggaagccg gtgagcgcgc gatccgggtt
      121 gacgatcacg ccgtgggcgc cgtacgagac ataggtggcc aacaccgtcg cgaacaccgc
      181 caccgccacc gacacggcca cttggcgctg ggtgctgaag atcgacgcgg ctcgtccgtt
      241 gtcggccggg gcgatggtgg cgtagctcgc cgcctgcatc ggcacgaagg cgaaccccat
      301 gcagaacccg cgtgcgaaca tcaggccgcg gatcacccac aagctggtgc cgagggtgat
      361 gaacgcgaac gcgccgatcg cgatcgcggc gccggccaga ccagcggcga tcagtcgccg
      421 cgggccgatg cggagataga cccgtcccgc gatcaccgac gcgacggcga ccccgatcgc
      481 ttgcgggaag gtggtcaggc ccgactcttg agccgacatg ccgcgcaacg actggaggta
      541 cagcgggatc acgaacatga tgcccatgaa gctgccggtg gtcatcacgc tgacgaggtt
      601 ggtctggcgg aacaagcggt cctgcaacag ccggagctgc agcatcggcg tcgaccggcg
      661 caactcgacc accaccatca aggcgatcac cgccagcccg ccgaggccgg tgatcagcac
      721 gaccggcgac gtccagcccg accgcggccc gtcgctgacg ccgtagacga acccggcgaa
      781 gccgatcgcg gagagcaaga acccaggcag gtcgaagttg ccggccgtcg gttcacggtg
      841 ctcgtgcagc aacgtgccgc cgatcgccag cgcgagcagt ccgatcggga tgttgatcgt
      901 gaagatccaa cgccagctga gatgggtaac gagcacaccg ccgatgatcg gccccagcgc
      961 cggtgcggcc atcgcgggga ccatcatcac cacggccact cgcgcccgct cggcgggcgg
     1021 gaagacccgg aacagcatcg ccatgccgac cggggtcaac atgccaccgc cgacgccctg
     1081 cagcacccgg aacgcgatca gttggccaac cgactgcgac agggcgcaca gcgcggacgc
     1141 aacgatgaac atgccgaacg cgaacatgaa gatccgcttg ttgcccatgc ggtcgctgag
     1201 ccacccggag accgggatga acacggccag gctgagggtg tagccgatca cggtccactc
     1261 ggtggccgac tcgccgaact cgctgaccag cgtgggcaga gcgacgttga cgatcgtggt
     1321 gtcgaggatg tcgaggaaca gcgccgacac cagcaccgcg gttacgaccc acttgtattc
     1381 gaactcgcgc cccaagaaac gcacgggctc gcggctgtcg atcgtgagcg ctcccggcgg
     1441 ctccggtgat cgtggcggtt cagacggcgc ggacgtcgcg gggccagccg ggggtgtact
     1501 caccgggccg acggtatcgc tgcctctcac acggtgacgg gtcgggacac cgccgcccgg
     1561 cccgtcaccg gcgcatgtca cacaccgacg ctccggtcat gccggcgtct cggcccacgg
     1621 gcgtcgccgg cgccgcggca tcgggtcgag tcgctggcgc gatggtcgaa tcggtggtcg
     1681 ctcacgtcag actgtgggcc atggcaaccg acgccacgcc cgccgcactc cagacgagcg
     1741 tgcccgacac cgaatggggc gcgttcacgg cgacgggtcc gtggtcgctc gatcccggca
     1801 ccatcggttg gctcccgct
//
LOCUS       DAORNF010000707         1816 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_46921_length_1816_cov_1.216354, whole genome shotgun sequence.
ACCESSION   DAORNF010000707 DAORNF010000000
VERSION     DAORNF010000707.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46921_length_1816_cov_1.216354"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>247)
                     /locus_tag="PLV68_20260"
     CDS             complement(<1..>247)
                     /locus_tag="PLV68_20260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03641.1"
                     /translation="PDVTRVLTEDGLQSSELDVITQFLGEVTFEDPRFVYPLIDVVVV
                     VGKSYLADMADARDDKGDTAGTDAGAGDGGGDPRQTSA"
     assembly_gap    248..336
                     /estimated_length=89
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<337..>730)
                     /locus_tag="PLV68_20265"
     CDS             complement(<337..>730)
                     /locus_tag="PLV68_20265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03642.1"
                     /translation="LVARDPSQPEARRLPNVRQVWNAISAAVGSGIEPRAPLDTAIAD
                     FEAYSNHFLAGTVQVYNELDVAPVAAADNPDGLDVTSLNVSSTLVLMASIAPSAMITP
                     FDTLNFRIENGLTQADIDAAGLVGVTNAD"
     assembly_gap    731..740
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<741..>1250)
                     /locus_tag="PLV68_20270"
     CDS             complement(<741..>1250)
                     /locus_tag="PLV68_20270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03643.1"
                     /translation="LQNSEAGNQVDRLPVVPFPSTPTAMIATTDAQGVVTAVAVLVMA
                     PSADGKEQAVGGTIVSLPTSVNTSGGGEAARPLASSLAEGGEERLLADVESASRVTVN
                     YHAVFDQDQMTAWMSRIGPLRVSLPNDVVTVNDAGDAVTLFAAGQQTLQPADVAAVLA
                     LAKKKGLEAN"
     assembly_gap    1251..1273
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1362..>1550)
                     /locus_tag="PLV68_20275"
     CDS             complement(1362..>1550)
                     /locus_tag="PLV68_20275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03644.1"
                     /translation="DRPGQPVVLPAEFPWHRVEVPRLEVSSTDLRARFVDGRPLDYLL
                     TQPVLDTIEARQLYGVAS"
     assembly_gap    1551..1560
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1561..1803)
                     /locus_tag="PLV68_20280"
     CDS             complement(<1561..1803)
                     /locus_tag="PLV68_20280"
                     /inference="COORDINATES: protein motif:HMM:NF013621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03645.1"
                     /translation="MTPAADRLAMVEAAVADVPGLVAGRDEIDRGGASYTADTLSVLA
                     TRFPKAALFTIVGDDAAAGITTWERHEEVVANSHLVV"
BASE COUNT          253 a          575 c          580 g          276 t
ORIGIN      
        1 cggcactggt ctggcgcgga tcgccgccgc cgtcgcccgc gcctgcatcg gttccagccg
       61 tgtcgccctt gtcgtcgcgg gcgtcggcca tgtcggccag atagctcttg ccgaccacga
      121 ccaccacgtc gatcagcggg tagacgaacc gtgggtcttc gaaggtgacc tcgccgagga
      181 actgggtgat cacgtcgagt tcggacgact gcaacccgtc ctcggtcaac acccgggtca
      241 cgtcgggnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngtcg gcgttggtca cccccaccag
      361 gccggctgcg tcgatgtcgg cctgggtgag gccgttctcg atgcggaagt tgagggtgtc
      421 gaacggggtg atcatcgccg acggggcgat gctcgccatc aacaccaacg tcgaggacac
      481 gttgagcgag gtgacgtcga gcccgtcggg gttgtcggca gcggccacag gagccacgtc
      541 gagctcgttg tagacctgca cggtgcccgc caggaagtgg ttggagtacg cctcgaagtc
      601 ggcgatcgcc gtgtcgagcg gtgcccgcgg ctcgatgccc gacccgaccg cggcggagat
      661 cgcgttccac acctggcgga cgttgggcaa ccggcgggcc tcgggctggg acggatcacg
      721 ggcgaccagc nnnnnnnnnn attggcttcc aggcccttct tttttgcaag cgccagcacg
      781 gcggcgacat cggctggctg cagggtctgt tgtccggccg cgaacagcgt cacagcgtcg
      841 cccgcatcgt tgacggtgac cacgtcgttg ggcaggctca cccgcagcgg gccgatcctg
      901 ctcatccatg cggtcatctg gtcctggtcg aacacggcgt gatagttgac cgtcacccgg
      961 ctggccgatt cgacgtcggc cagcaagcgc tcttcgccac cctcggccag gctggacgcg
     1021 agcggccgtg ctgcctcgcc cccgcccgag gtgttgacgc tggtcggcag cgacacgatc
     1081 gtgcctccga cggcctgctc cttgccgtcg gcgctgggcg ccatgaccaa cacggctacc
     1141 gccgtcacca cgccctgagc atcggtggtg gcgatcatcg ccgtcggtgt cgacgggaac
     1201 ggcaccaccg gcaggcggtc gacctggttc ccggcctcgg agttctgcag nnnnnnnnnn
     1261 nnnnnnnnnn nnntaggtga gtaccggagc cgcacccagc gcgagcaggc ccgacaacgc
     1321 cgcggcgatg gtgcggttgc gacgacggct cgccgaagcg gtcacgatgc gactccgtac
     1381 agttggcgtg cctcgatcgt gtcgagcacg ggctgggtca acaggtagtc gagcggtcga
     1441 ccgtcgacga agcgtgccct caaatcggtg ctggacacct ccaagcgggg tacttcgaca
     1501 cggtgccacg ggaactcggc gggcagcacc accggctggc ccgggcggtc nnnnnnnnnn
     1561 gaccaccagg tgcgagttgg cgacgacctc ttcgtggcgc tcccaggtcg tgatacccgc
     1621 cgcagcgtcg tcgccgacga tggtgaacag cgccgccttg ggaaaccggg tcgccaacac
     1681 gctcagcgtg tcggccgtgt agctcgcacc gccgcgatcg atctcgtccc ttccggcaac
     1741 caagccgggt acgtcagcca cggccgcctc caccattgcc agccgatccg cggcgggtgt
     1801 gatctggcgg ctgtgc
//
LOCUS       DAORNF010000708         1815 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_46938_length_1815_cov_1.990341, whole genome shotgun sequence.
ACCESSION   DAORNF010000708 DAORNF010000000
VERSION     DAORNF010000708.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1815
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46938_length_1815_cov_1.990341"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /locus_tag="PLV68_20285"
     CDS             <1..585
                     /locus_tag="PLV68_20285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03646.1"
                     /translation="VLRQLTTDHSLVQEMVDEGTVAPEAAKNHPQRNVVTRAVGIEPA
                     LVADFVVVPTVPECRLLLCSDGAHGELDDEVLTDLLTGEGDAQATAEAIVAAVLSGRA
                     PDNVTAVVVDLVCAAATADDDLYVTGPAAERDLEITAPRPRPTRARVSTATPPPGDAD
                     SVLISVVPTDESGSPTAQDVVEPDPPALIDEVPL"
     gene            684..>1815
                     /locus_tag="PLV68_20290"
     CDS             684..>1815
                     /locus_tag="PLV68_20290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03647.1"
                     /translation="MNDAGEHGWTADGATGPIGDTSGWRVGHGIGLIAPSTLAVLEPV
                     NDRLGAQLWAIIERGGDVDELLDAVSSGGLRTLGSFAMAQYEPNTLRIVVRGHGTADV
                     TTAAGERHIEASGARTWVETTVADATSVVLRLGAPLAASLPLRVTAGIVPADLVARGV
                     GDTAKATAGAEPSTGLVDLDAIDFSAVASLDPALIVASAPLPAPGTMAHSASRDAATL
                     ASDEPASDEPASDESASDGPASDEATGGEPAGAPRLRQPWPDADYSAPPDLGPLLATP
                     SQITALGDLGATVAPRAPETLVDLSSDAPHAANDDVLGTDDAVADDRGDATTSDGPDP
                     NLTLAASNFDGPSVDDHFDEIFGNTMAKSVQAAAVMPAEPVHD"
BASE COUNT          276 a          617 c          659 g          263 t
ORIGIN      
        1 gtgctgcgcc aactgaccac cgatcactcg ctggtgcagg agatggtcga cgagggaacg
       61 gtcgcgccag aggcggccaa gaaccatccg cagcgcaacg tggtgacgcg ggccgtcggc
      121 atcgagccag cactcgtcgc cgacttcgtc gtggtgccga cggtgcccga gtgccgtctg
      181 ctgctgtgtt ccgacggggc gcacggcgag ttggacgacg aggtgctgac cgatctgctc
      241 accggggaag gcgacgcgca ggcgacggcc gaagcgatcg ttgcggccgt gctctccggc
      301 cgcgcgcctg acaacgtcac ggccgtcgtg gtcgatctgg tctgcgcggc cgcgaccgcc
      361 gacgatgatc tgtacgtcac cggaccggcc gcagagcgcg acctggagat caccgcgccc
      421 cgcccccgtc ccacccgggc gcgggtgtcg acggcgactc caccgcccgg cgacgccgat
      481 tcggtgctga tctccgtggt gcccaccgac gagtccggct ccccgaccgc tcaagacgtg
      541 gtcgaaccag acccccctgc cctcatcgac gaggtgccgc tgtgaaccga cgcgagcgtc
      601 agcgagcgga cgacgacggc gcgagcgcca gcgagcctgg cgcaaatgtc gacagcgggg
      661 ctgtggtcgg ggatcatgtc gccatgaacg atgcgggcga gcatgggtgg acggcagatg
      721 gtgcgacggg accgatcggt gatacgtccg gttggcgggt ggggcacggc atcgggctga
      781 tcgcgccgtc gaccctggcg gtgttggagc cggtcaacga tcggctcggg gcgcaactgt
      841 gggcgatcat cgaacgtggc ggcgatgtgg acgaactgct cgacgcggtg tcctcgggcg
      901 ggttgcgcac gctcgggtcg ttcgcaatgg cgcagtacga gccgaacacg ctgcggatcg
      961 tggtccgcgg gcacggcaca gccgatgtga ccaccgcagc cggcgagcga cacatcgagg
     1021 ccagcggcgc tcggacatgg gtcgagacga cggttgccga tgcgacatcg gtggtgttgc
     1081 gcctcggcgc gccgttggcc gcgtcgctgc cgctgcgggt gacggcaggc atcgttccgg
     1141 ccgaccttgt tgctcgcggc gtgggcgaca ccgccaaggc cacggctggc gcggaacctt
     1201 cgactggcct ggtcgatctc gacgcgatcg acttttccgc ggttgcctcg ctcgaccccg
     1261 ccttgatcgt tgccagcgca ccgctgcccg cgcccgggac catggcccat tcggcatccc
     1321 gtgatgctgc gacgctcgcc agcgatgagc cggccagcga tgagccggcc agtgacgagt
     1381 cggccagcga tgggccggcc agtgacgagg caaccggtgg tgagccagcc ggtgcgccga
     1441 ggctgaggca accgtggccg gacgctgact acagcgcgcc gcccgatctc ggtccgctgc
     1501 tggcgacgcc gagccagatc actgcgctgg gtgacctcgg tgcgaccgtg gcgccgcgtg
     1561 cgccggagac gctggtcgac ctgtcgtccg acgcgccgca tgcggcgaat gacgacgtcc
     1621 tcggcacgga tgacgccgtt gccgacgacc ggggcgatgc gaccacgtcc gacggaccgg
     1681 accccaacct gaccttggcc gcctcgaatt tcgacggacc gagcgtcgac gaccacttcg
     1741 acgagatctt cggcaacacg atggccaagt cggtgcaggc cgcggcggtc atgcccgcgg
     1801 aaccggttca cgact
//
LOCUS       DAORNF010000709         1814 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_46969_length_1814_cov_1.632177, whole genome shotgun sequence.
ACCESSION   DAORNF010000709 DAORNF010000000
VERSION     DAORNF010000709.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1814
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46969_length_1814_cov_1.632177"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>330
                     /locus_tag="PLV68_20295"
     CDS             <1..>330
                     /locus_tag="PLV68_20295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03648.1"
                     /translation="GAGSAAASGGRPDTELDHVIERQDVAWHERPDDLSAFSRRLGPG
                     QAVRTAADHGLTADGVAAGLVDHGPVDHGPVDDGPVRSGEPVRLMGRLARAGVSGQVA
                     LAERKPD"
     assembly_gap    331..377
                     /estimated_length=47
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <378..1199
                     /gene="lipA"
                     /locus_tag="PLV68_20300"
     CDS             <378..1199
                     /gene="lipA"
                     /locus_tag="PLV68_20300"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HRE03649.1"
                     /translation="KKTIRDLGLITVCEEAGCPNLSDCWSDGTATFMVLGERCTRACG
                     FCLVDTRKPQPPDAGEIERVAEAVARMQLDHAVLTMVARDDLADGGMAHVAACVRAIR
                     DRRPGTRIETLISDAKGEPTSLDLVFAAAPDVMNHNVETVPRLQRAVRPSANYARSLS
                     VLSRAKLAGLTVKSGLIVGMGETPAEVEGVLTDLASIGTDIVTIGQYLRPTSHHLPVH
                     RWVEPGEFDRWKAFGEAAGIGHVEASPLTRSSYHAKQAAEAVTPVAVTPIALATR"
     gene            1342..>1814
                     /locus_tag="PLV68_20305"
     CDS             1342..>1814
                     /locus_tag="PLV68_20305"
                     /inference="COORDINATES: protein motif:HMM:NF013486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HRE03650.1"
                     /translation="MNTSGAATPLSTQVYATRMERARELMAERDVDVLLLSLGLDLPY
                     ISGYTAMPLERLTMLVVPREGAATMVIPRLEAPRVTAQPGVFDLHPWNETENPVEIVA
                     NLVRAGGPVRRVAVGDQTWARFVVELIPLLAGATFMRADVVSALRLVKDQTEIDAL"
BASE COUNT          256 a          611 c          627 g          273 t
ORIGIN      
        1 tgggcgccgg ctcggccgcc gcgtcgggag gtcggccgga taccgagctg gaccacgtca
       61 tcgagcgcca agatgtggcc tggcacgaac gtcccgacga cctgtcggcg ttctcccgcc
      121 gcctcggccc cggccaagcc gtgcgcaccg cggccgatca cggcctcacc gcggacggtg
      181 tcgctgctgg actggtcgat catggcccgg tcgatcatgg accggttgat gatggcccgg
      241 tccgtagcgg tgagccggtg cgcttgatgg gtcgtctcgc ccgggcgggg gtcagcgggc
      301 aggtggcgtt ggccgagcgc aagcccgact nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnaag aagacgatcc gcgatctcgg cttgatcacg gtgtgcgaag
      421 aggcgggctg ccccaacctc agcgattgtt ggagcgacgg cacggccacg ttcatggtgc
      481 tcggtgagcg ctgcaccagg gcttgcgggt tctgcttggt cgacacgcgc aagccgcagc
      541 cgcccgatgc cggtgagatc gaacgggttg cggaggcggt cgcgcggatg cagctcgatc
      601 acgcggtgct cacgatggtg gcccgtgacg acctggctga cggtggcatg gcccacgtcg
      661 cagcgtgcgt ccgggccatc cgcgatcgcc gccccggcac gcgcatcgag acgttgatct
      721 ccgacgccaa gggcgagccg acctcgctcg acctggtgtt cgcggccgcc cccgacgtga
      781 tgaaccacaa cgtcgaaacg gttccccgtc tgcagcgcgc ggtccgtccg tcggccaact
      841 acgcccgcag cctcagcgtg ctgtcccggg ccaagctggc cggcctcacc gtcaagtcgg
      901 gactgatcgt cggcatgggc gagacgcccg ccgaagtcga gggggtgctc accgatctgg
      961 cgtcgatcgg caccgacatc gtcaccatcg gtcaatacct gcgccccacc tcgcatcacc
     1021 tgccggtgca tcgctgggtc gagccgggtg agttcgatcg ctggaaggcg ttcggtgaag
     1081 cggcgggcat cggccacgtc gaagcgagcc cgctgacccg cagcagctat cacgccaagc
     1141 aagccgccga agccgtcacc ccggtcgccg tcacccctat cgctctcgcc acccgctgac
     1201 gccccgttcc gccgcacccc ggcgatttcg gcgtccgcgg agcggggagt ggccgctttc
     1261 cggcgagtcc gtactccgcg gcagccggat tcaccgggac ggcggcgggc ggaggtgggt
     1321 gggcgggcag aatgggcggc gatgaacacg tccggcgccg ccacaccgtt gtccacccag
     1381 gtctacgcca cccgcatgga gcgggcacgc gagttgatgg ccgaacgtga cgtcgacgtg
     1441 ctgctgctgt cgctcgggct cgatctgccc tacatctccg gctacacggc gatgccgctc
     1501 gaacggttga cgatgctggt cgtgccccgc gagggcgcgg cgacgatggt gatcccgcgc
     1561 ctcgaagcgc cgcgggtcac cgctcagccg ggggtgttcg acctgcaccc gtggaacgag
     1621 accgagaacc cggtcgagat cgtcgccaac ctggtccgcg cgggtggacc ggtgcggcga
     1681 gtcgcggtcg gcgatcagac ctgggcccgg ttcgtggtcg aactgatccc gttgctcgcc
     1741 ggggcgacgt tcatgcgcgc cgacgtcgtc agtgcgctgc gcctggtgaa ggatcagacc
     1801 gagatcgacg cgct
//
LOCUS       DAORNF010000710         1812 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_47031_length_1812_cov_1.582812, whole genome shotgun sequence.
ACCESSION   DAORNF010000710 DAORNF010000000
VERSION     DAORNF010000710.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1812
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47031_length_1812_cov_1.582812"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..530
                     /locus_tag="PLV68_20310"
     CDS             <1..530
                     /locus_tag="PLV68_20310"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: protein motif:HMM:NF014347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HRE03651.1"
                     /translation="VLGEMGIGNTTAAAAVAAALGGGEVAAWVGRGTGVDDEGLVRKR
                     EAVRAAVARVVGVLDPLEVLRELGGAELVAMAAAIVAARHRRLPVLIDGYVVTAAALP
                     LSCLAPDALDHCLVGHCSAEPGHRRLLQRLGKEPLLDLEMRLGEGSGAMAAVPLVAMA
                     CAGVTEVPTFGEWFG"
     gene            complement(593..>1199)
                     /locus_tag="PLV68_20315"
     CDS             complement(593..>1199)
                     /locus_tag="PLV68_20315"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRE03652.1"
                     /translation="GKTTLALHLNGLVSIQQGEIMVGTTSLTAPNVTEVRRRVGLVFQ
                     DPDDQLFMGSVRDDVGFGPANLGLRGAERSRRVDEALDAVGATPLAERHPHHLSGGEK
                     RRIAIATVLAMKPEVIVLDEPTSGLDPVGRRELAELLATIDATQIVVTHDLPFVLALC
                     PRSVVLDGGRIVADAPTRELFGDEALLRRHRLELPLGFRLA"
     assembly_gap    1200..1237
                     /estimated_length=38
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1313..>1574)
                     /locus_tag="PLV68_20320"
     CDS             complement(1313..>1574)
                     /locus_tag="PLV68_20320"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CbiQ family ECF transporter T component"
                     /protein_id="HRE03653.1"
                     /translation="PRWLWQARPIAASLGTLFVRSYEHGERVHLAMLSRGFTGSMPSL
                     DDDPSQRRRPLLARSLLARSAVLAPACLSLAVAIAALCGVGT"
     assembly_gap    1575..1593
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1594..>1812)
                     /locus_tag="PLV68_20325"
     CDS             complement(<1594..>1812)
                     /locus_tag="PLV68_20325"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbiQ family ECF transporter T component"
                     /protein_id="HRE03654.1"
                     /translation="GVAVVRPGLWAAWNLAAKVVLGATASIVLTATTTVADVLRGLTA
                     LRVPRVLVAIIAMAFRSLDILVDRLGTMR"
BASE COUNT          269 a          624 c          619 g          243 t
ORIGIN      
        1 tggtactggg tgagatgggc atcggcaaca ccaccgccgc tgcggccgtg gccgctgccc
       61 tcggcggtgg cgaggtcgcc gcgtgggtcg ggcgtggcac cggcgtcgac gacgaaggcc
      121 tggtccgcaa acgcgaagcc gtgcgcgccg cggtggccag agtggtcggc gtgctcgacc
      181 cgctggaggt gttgcgggaa ctgggcggcg ccgagttggt cgcgatggcc gcggcgatcg
      241 tcgccgcccg tcaccggcgc ttgcccgtgc tgatcgacgg gtacgtcgtc acggctgccg
      301 cactgccgtt gtcctgtctg gcccccgatg ccctcgacca ctgcctggtc ggacactgct
      361 cggccgagcc cggtcaccgg cggctgttgc agcgcctcgg caaggagccc ttgctcgacc
      421 tggagatgcg cctgggcgag ggcagcggcg caatggccgc ggtgccgctg gtggcgatgg
      481 cctgcgcggg cgtcaccgag gtccccacgt tcggcgagtg gttcggctga atatggttcg
      541 gctgagcgtg tctcggctga gtgtggttcg gctgggcttc gggctgaccg attcaggcca
      601 agcggaagcc gagcggtagc tcgaggcggt ggcggcgcag caacgcctcg tcgccgaaca
      661 gttcgcgggt cggggcatcg gcgacgatcc ggccgccgtc gagcacgacc gaacgcgggc
      721 acagagcgag cacgaacggc aggtcgtgcg tgacgacgat ctgcgtcgcg tcgatcgtcg
      781 cgagcagctc cgccaactcg cgacgcccga ccgggtcgag tcccgacgtc ggctcgtcga
      841 gcacgatcac ctcgggcttc atcgccaaca ccgtcgcgat cgcgatgcgg cgcttctcgc
      901 ccccgctcag gtgatgcgga tggcgctcgg ccagtggggt cgcacccacc gcgtcgagtg
      961 cctcgtccac ccgccgggaa cgctcggctc cgcgcagacc gaggttggcc gggccgaagc
     1021 cgacgtcgtc gcgcaccgac cccatgaaca actggtcgtc cgggtcttgg aacaccagac
     1081 cgacccggcg ccggacctcg gtcacgttgg gcgcggtcag cgatgtggtc ccgaccatga
     1141 tctcgccctg ctggatcgag accagcccgt tgagatgcaa ggcgagcgtg gtcttgccgn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncga ggccgtgcaa caccaactcg
     1261 ctgtgccccg gatagcggaa cgacacaccg cgcacgctga cccgcgccgc gctcacgtcc
     1321 cgaccccgca cagtgcggcg atcgccacgg ccaggctcag acacgcgggg gcgagcaccg
     1381 ccgaccgtgc cagcagggac cgtgccagca gcggtcggcg ccgttgcgac gggtcgtcat
     1441 cgagcgacgg catcgagccg gtgaagccgc gcgacaacat cgccaagtgg acccgctcgc
     1501 catgctcgta cgagcgcacg aacagcgtgc ccaacgacgc cgcgatcggc cgtgcctgcc
     1561 acagccaccg cgggnnnnnn nnnnnnnnnn nnngcgcatc gtccccaaac gatcgacgag
     1621 gatgtcgagc gaacggaacg ccatggcgat gatcgccacc agtacccgtg gcacacgcaa
     1681 cgcggtcagc ccacgcagca cgtcggccac cgtggtcgta gcggtcagca cgatgctcgc
     1741 tgttgcaccc aacacgacct ttgcagccag gttccacgcg gcccacaggc ccggccgaac
     1801 gacggccacg cc
//
LOCUS       DAORNF010000711         1811 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_47066_length_1811_cov_1.853645, whole genome shotgun sequence.
ACCESSION   DAORNF010000711 DAORNF010000000
VERSION     DAORNF010000711.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47066_length_1811_cov_1.853645"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..559
                     /locus_tag="PLV68_20330"
     CDS             <1..559
                     /locus_tag="PLV68_20330"
                     /inference="COORDINATES: protein motif:HMM:NF021430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2135 domain-containing protein"
                     /protein_id="HRE03655.1"
                     /translation="AVHLAREDWRRAMDHLAVVVKGKWDRFQGIEVIALHELNRFWLE
                     AKAAGVSGGFPLDNRFEHAMQLDTRIVMTWDADMTDMDLHVVEPSGEEAYYSHNLTTI
                     GGKVSRDFTQGYGPEIYAVKKAMRGTYKVKTKFFGSSAAQLIGAVTLQVDVFTNWGRD
                     NEKRRSMTLRLTESKEEFVVGEIEF"
     gene            complement(668..>1811)
                     /locus_tag="PLV68_20335"
     CDS             complement(668..>1811)
                     /locus_tag="PLV68_20335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03656.1"
                     /translation="SDEEILDRRDENQKVVGLITEEVFRSSGVVTDYLTAAVPLGAAE
                     PFAASFTCATCGSDVDTSCNGLDDDCDGQTDEDYVVTSSNCGMGVCAASGLVTCQGGV
                     LVDTCAPGSPASWTNTFDTLDDADDVYVSVYRGTQFCDAITGTGPGLTCAPEPGFSAT
                     GGFSGGYVYGSAQDNLLRHYGLTVDAVQLPNLLGGQLSARFRTFNRDQSGPGSVKAAE
                     GSSNLAANATTATARARWIITARNPSTNAIWIWVSRDDFAWNPNDDTTWTSRSIPVSW
                     ANFAQWQPFMTNTYGGGSPAEFADSVNNVIRVGVIMSDASIVYASHLHASWFRTAKYG
                     VMSASLPTGIELGWDELTLSSTGSDCNSNGLDDDCNGTIDDCTAAF"
BASE COUNT          294 a          599 c          622 g          296 t
ORIGIN      
        1 ggccgtccac ctcgcccgcg aggactggcg ccgcgccatg gaccacctgg ccgtggtcgt
       61 gaagggcaag tgggaccgct tccagggcat cgaggtcatc gcgctccacg agctgaaccg
      121 cttctggctc gaggccaagg cggcgggcgt gagcggcggc ttcccgctcg acaatcgctt
      181 cgaacacgcg atgcagctcg acacccgcat cgtcatgacc tgggacgccg acatgaccga
      241 catggacctg cacgtggtcg agccgtcggg cgaggaggcc tactactcgc acaacctgac
      301 gaccatcggc ggcaaggtga gccgcgactt cacgcagggc tacgggcccg agatctacgc
      361 cgtcaagaag gccatgcgcg gcacctacaa ggtgaagacc aagttcttcg gcagctcggc
      421 cgcgcagctc atcggcgccg tcaccctgca ggtcgacgtc ttcaccaact ggggccgcga
      481 caacgagaag cgtcgctcca tgaccttgcg cctcaccgag tcgaaagaag agttcgtcgt
      541 cggcgagatc gagttctgag atcgcccagg cgccacccgc ctggggactt gcgccgcgcc
      601 gcgtgaccgg tctcgccggc ctccgcctct ctcctgaaga ggcggcccgg cccggaacac
      661 gccggtctca gaacgcggcg gtgcagtcgt cgatggtgcc gttgcagtcg tcgtcgaggc
      721 cgttgctgtt gcagtcgctg ccggtcgagc tcagcgtgag ctcgtcccag cccagctcga
      781 tgcctgtcgg caggctcgcc gacatgacgc cgtacttcgc cgttcggaac caggaggcgt
      841 ggaggtgtga cgcgtacacg atcgacgcgt ccgacatgat gacgccgacc cggatgacgt
      901 tgttgacgga gtcggcgaac tcggccggac tgccaccgcc gtaggtgttg gtcatgaacg
      961 gctgccactg cgcgaagttg gcccagctca cggggatgga gcgcgaggtc caggtcgtgt
     1021 cgtcgttggg gttccacgcg aagtcgtcgc gcgataccca gatccagatg gcgttcgtgc
     1081 tcggatttcg cgcggtgatg atccagcgag cgcgcgccgt cgccgtggtg gcgttggccg
     1141 cgagattgct cgagccctcg gccgccttga ccgagccagg cccgctctgg tcgcgattga
     1201 aggtgcggaa acgcgccgac agttggccgc cgagcaggtt ggggagctgc acggcgtcga
     1261 cggtcaggcc gtagtggcgc aagaggttgt cctgcgccga cccatagacg tagccgccgc
     1321 tgaagccgcc cgtcgcgctg aatcccggct ccggcgcgca ggtgagtccc ggccccgtgc
     1381 ccgtgatggc gtcgcagaac tgcgtcccgc gatagaccga gacgtagacg tcgtcggcgt
     1441 catcgagggt gtcgaaggtg ttggtccacg aggccggcga gcccggcgcg caggtgtcga
     1501 cgaggacgcc accctgacag gtgacgaggc cactcgccgc gcaaaccccc atgccgcagt
     1561 tcgaggaggt gacgacgtag tcctcgtcgg tctggccgtc gcagtcgtcg tcgaggccgt
     1621 tgcagctcgt gtcgacgtca ctgccacagg tggcacatgt aaacgacgcg gcgaagggct
     1681 cggccgcgcc cagcggcact gcggccgtga ggtagtcggt cacgaccccg ctgctgcgga
     1741 aaacctcctc ggtgatgaga cctacgacct tctggttctc gtcgcgacgg tcgaggattt
     1801 cctcgtcgct t
//
LOCUS       DAORNF010000712         1807 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_47209_length_1807_cov_1.400114, whole genome shotgun sequence.
ACCESSION   DAORNF010000712 DAORNF010000000
VERSION     DAORNF010000712.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1807
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47209_length_1807_cov_1.400114"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    286..440
                     /estimated_length=155
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<441..650)
                     /locus_tag="PLV68_20340"
     CDS             complement(<441..650)
                     /locus_tag="PLV68_20340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03657.1"
                     /translation="MAGVELHQACGDDEVAELFAVRYGLDLDASEVMEHKEKLYLQLL
                     PTVKVIEPVVEAARRAAARHPVSIAT"
     gene            complement(748..>946)
                     /locus_tag="PLV68_20345"
     CDS             complement(748..>946)
                     /locus_tag="PLV68_20345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03658.1"
                     /translation="RLLYWCPGCQARGDSRVNPTPPLGIDRPMDPHPAAVMYVSDLPW
                     RRHDDTGPVQAPKPTRHTATG"
     assembly_gap    947..956
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<957..1724)
                     /locus_tag="PLV68_20350"
     CDS             complement(<957..1724)
                     /locus_tag="PLV68_20350"
                     /inference="COORDINATES: protein motif:HMM:NF018533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03659.1"
                     /translation="MPEGDIHRATIALRTALVGKPTLAFRARGLLGPAPTIGRTIESV
                     ECHGRHVEIVWDDGLTLHSNLRLSGVWHLYRSTEMWRRRTDQMRVSIEIDGWVAVCFS
                     APVVETYRQFDRHRHPGSGRLGPDLCRDRADRVGQREAVDRLVAYPDPAAFVGEALLD
                     QHVARGIGNVFRSEVLWAVELSPFAAVGALTRDDATAVIAEAVRLLQDNISGDYSRRV
                     HRLGVASSRGHHPGLGVYGRNGQRCDRCGDTVQVKRVA"
BASE COUNT          263 a          568 c          559 g          252 t
ORIGIN      
        1 gctgtgcccg ggtatccgcg tgatgttcat gtcagggtac gtgggcgatc cgtccatcct
       61 ccaggcggtt caggatgccg gggcgcgctt cctcgagaag cctttcaccc ggtccagcct
      121 ggctcgaaaa gtgcgtgagg cactcgagcc gccggtgcga taacccccgc accgcccatc
      181 aggggcggct gtcagaacgg cttgtccgtc tagggacacg tacggtggcc atcccagcgg
      241 cgcgcgccgc cgccaatcct ggctccgcat cctcaaacac gaggcnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn agttgcgatc gagacaggat gtcgtgccgc cgcgcgtcgg
      481 gcggcttcca ccacgggttc gatcactttc accgtcggga ggagctgaag gtagagcttc
      541 tctttgtgct ccattacttc gctcgcatcg agatccaaac cgtagcgaac ggcaaacaac
      601 tcagcaacct cgtcatcgcc gcaggcctga tgcagttcga cccccgccaa ctcgacaccc
      661 cgtgacaccc cctcccacct gacgatgacg ccgcaggtgt tgccgtcgca gcagaccgcc
      721 gaagcgatcc gagccgctgc gggggtgtca gccggtggcg gtgtggcggg tgggtttggg
      781 tgcctggacc gggccggtgt cgtcgtggcg gcgccatggg aggtcggaga cgtacatcac
      841 cgcggccggg tggggatcca tcgggcggtc gatgccgagc ggcggggttg ggttcacccg
      901 cgagtcgccg cgggcctggc aaccggggca ccagtagagc aggcggnnnn nnnnnncgcc
      961 acccgcttga cctgcacggt gtcgccgcac cgatcgcagc gttggccgtt gcggccgtac
     1021 accccgagcc cggggtgatg gccccggctc gatgccacac ccagacggtg gacgcgtcgg
     1081 ctgtagtcgc cgctgatgtt gtcttgcagc aaccgcaccg cttcggcgat caccgcggtt
     1141 gcgtcgtcac gggtcaacgc cccgacggct gcgaacggac tgagctcgac cgcccacaac
     1201 acctcgctgc ggaacacgtt gccgatgccg cgggcaacgt gctggtcgag cagcgcctca
     1261 ccgacgaagg ccgcgggatc gggatacgcg acgaggcgat cgacggcttc gcgctggccg
     1321 acacggtcag cgcggtcgcg gcacaggtcg gggccgagac gccccgaacc gggatgacgg
     1381 tggcggtcga actggcgata cgtctcgacc accggcgcgc tgaagcacac cgcgacccag
     1441 ccgtcgatct cgatcgacac ccgcatctgg tcggtacggc gccgccacat ctcggtggac
     1501 cggtagaggt gccatacgcc ggacaggcgc aggttggagt gcagggtcag cccgtcgtcc
     1561 cacacgatct cgacgtggcg gccgtgacac tcgacgctct cgatcgtacg accgatggtg
     1621 ggcgccgggc cgagcagccc acgagcgcgg aaggcaaggg tcggctttcc caccagcgcc
     1681 gtccgcaacg cgatcgttgc ccgatggatg tccccctctg gcacaagcgc tacagtaccc
     1741 cgccccgtcg ggccgattgt gcgatgcctg cgcccgcgac accgccgaac cgctcggtgg
     1801 cggatgg
//
LOCUS       DAORNF010000713         1803 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_47320_length_1803_cov_1.770595, whole genome shotgun sequence.
ACCESSION   DAORNF010000713 DAORNF010000000
VERSION     DAORNF010000713.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1803
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47320_length_1803_cov_1.770595"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>354
                     /locus_tag="PLV68_20355"
     CDS             <1..>354
                     /locus_tag="PLV68_20355"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HRE03660.1"
                     /translation="TPDAEAVDADQLAALCAAGEAQIVVPNVAGMSYAEAEAALVSLG
                     LHVTPIFEDSPLGATTDADPVTKQGTAPGAVVACDAGVTVGVAFQPGPLHVVQDGESL
                     ASISEAGGPTVQELA"
     assembly_gap    355..364
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <365..825
                     /locus_tag="PLV68_20360"
     CDS             <365..825
                     /locus_tag="PLV68_20360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03661.1"
                     /translation="AVDVDAEIAPGIALRLTPPPGLDLSLFDNGLGDVLIGSVDLDGE
                     YIGVEIIDGGNGLCLTLADGTRAGCNTIDVPGGEIQEAHFDAGDVIYGYSTGDKADLT
                     VTLDGKPLDIATSDPVPYGDQMAWMWVTRLPAPESATHIVVTIATAPETP"
BASE COUNT          294 a          649 c          577 g          273 t
ORIGIN      
        1 cgacgccgga cgccgaggcg gtcgacgccg atcagcttgc tgccctgtgc gccgctggcg
       61 aagcgcagat cgtcgtgccg aacgtggcgg gcatgtccta cgccgaggcc gaggcagcgc
      121 tcgtgtcgtt gggcctccac gtcacgccga tcttcgagga ctcccctctg ggagcgacga
      181 ccgacgccga cccggtgacg aagcagggca cggctcctgg cgcggtcgtc gcctgcgacg
      241 cgggtgtcac ggtcggtgtc gcgttccaac ccggcccgct ccacgtcgtc caagacggcg
      301 agagtctggc gtcgatcagc gaagccggcg ggccgacggt gcaagaactc gccgnnnnnn
      361 nnnnatgccg tcgacgtcga tgccgagatc gcgcccggca tcgccctgcg cctcactcct
      421 ccacccggtc tcgacctcag cctcttcgac aacggactcg gcgatgtact catcggctcg
      481 gtcgacctcg acggcgagta catcggcgtc gagatcatcg acggcggcaa cggcctgtgc
      541 ctcaccctcg ccgacggcac ccgcgccggt tgcaacacga tcgacgtacc gggcggcgaa
      601 atccaggaag cacacttcga cgctggcgac gtcatctacg gctactccac cggcgacaag
      661 gccgacctca ccgtcaccct cgacgggaag ccactcgaca tcgcgacctc cgaccccgtg
      721 ccctacggcg accagatggc ctggatgtgg gtcacccgcc tccccgcccc cgagtcagcc
      781 acccacatcg tcgtcaccat tgcgaccgcc cccgagacgc cgtaggtcat cgccgacggg
      841 caaggtcgta caacgtcgaa cgcgctgtcg ttgtacgcgc ttgtacgagg tcggtccggt
      901 cccgcgggtg gcaaacacga acgggacgga cagcgcaagc tgcaaggacc gccatcggca
      961 gtgacgacac gagccgagtt cgcggccgcg ctgggactgc tgcgcgatcg agcagggttg
     1021 tcactgcggc gactcggaca cgagtcgggc gtcccggcga caaccctcaa cggctggttc
     1081 accggccgcc accttccgtt cccgcgcgac aacgagttgc tcctcgcggt gatcgctcac
     1141 ctcggcgtga ccgacacgga accatggctg gacgcgctga tccgggtgcg gacgacgccg
     1201 gcgcgggcag cacgccaagc actgtgcact ctctcgggct ggccagctac gaggcgaccc
     1261 aagccgacct gttgtcggcc gcgagtcgct gatcgaacag ggactggccc agttcgacgc
     1321 gctggctggc gatccggccg tgctcgcggc actgttcgtg gtcggcccgt ctggatcggg
     1381 caagagctcg cttgtgcacg ccgagccgca gccgcgattc gaacagcgcg gcctcactgc
     1441 gatcgacgca cgcctggtac tgatcctgcg gctgcttctg gaccgccgac cccgacgccg
     1501 caatcgccca gcgctgcgcc caaaccggcg acaacctcac cccgaccgaa tggtcccagt
     1561 tcgtccaagg cgtcggtcgt cgtcgcgagc gtggccgctg gcgagacggt gacgatcacc
     1621 gaccgtggac ggcccgtggc acagatgaca ccgcctgatc acgtgcggtc atgtcgtcaa
     1681 tgccgacggc ggtccgtgca gcccgtggcc gcgctgatcc gggtgacgtt gctgcgcccg
     1741 atcactatcg acgaaatcga tatctgacgg ccccggtatc tcgaactgtg atgtgattcg
     1801 ggt
//
LOCUS       DAORNF010000714         1803 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_47330_length_1803_cov_1.389016, whole genome shotgun sequence.
ACCESSION   DAORNF010000714 DAORNF010000000
VERSION     DAORNF010000714.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1803
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47330_length_1803_cov_1.389016"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(159..>828)
                     /locus_tag="PLV68_20365"
     CDS             complement(159..>828)
                     /locus_tag="PLV68_20365"
                     /inference="COORDINATES: protein motif:HMM:NF023266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ERAP1-like C-terminal domain-containing protein"
                     /protein_id="HRE03662.1"
                     /translation="SDDEDDLRSKLRGLLVTTLAKIGRDRDAQQRCRDLLAALDANGA
                     TSSGNGVTADPELISAATTVVAATGDATDFERFVAAFEAATTPQESLRNLYALAEFDD
                     AALIERTCEFAFSGAVRSQNAPYLLGRCIANRHHGARAWTLVRKHWDQANAAFPNPSI
                     IRMVDPVKLLNTPDAVADVQGFFSEHPIKQSANTLAQVLERQQINAAVRDREAERFTA
                     TLAD"
     assembly_gap    829..844
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<845..1781)
                     /locus_tag="PLV68_20370"
     CDS             complement(<845..1781)
                     /locus_tag="PLV68_20370"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HRE03663.1"
                     /translation="MRWWNGIWLNEAFATFMEVMAVDAFRPQWQRWTNFSLERSDAFE
                     VDSLSSTRTVEFPVHAPAECEGMFDVLTYQKGGALLRMLQQYIGEERFRQGVNHYLRT
                     HSYGNTETGDLWDGIEAVTTADGGNEPVRHMMDSWIWQAGYPLVSASLRGSSLHLRQE
                     RFSFSTAADDPTLFMVPVAVRVGTGEQYVLLTDAETKIEIDDPDAVVVVNAGGQGFFR
                     VAYDDTLRGRLNGAALATLGTVERYNLVDDASAAFVAGRLDAAGLLQFLRGFDGERDL
                     AVWQAIVAALGRLHRVLDPAGEERFAAIAADLVRPA"
BASE COUNT          274 a          582 c          649 g          282 t
ORIGIN      
        1 acctggggcg ccatagagga agccggcgta gaccgcgacg ccgagcccga cgatgccacc
       61 gccggcggcg acagcccagc gcccgcggcg ggaacgtgag cgatcgacgg tggttggcac
      121 gccggccacg ctatccgcgt cgggtcgggg ggattccgtc agtcggccaa cgtggccgtg
      181 aatcgctcgg cctcgcggtc tcggaccgcg gcgttgatct gctggcgctc gagcacctgg
      241 gccagggtgt tggccgactg cttgatcggg tgctcgctga aaaagccctg cacgtcggcg
      301 accgcgtcgg gggtgttcag gagcttgacc ggatcgacca tgcggatgat cgaggggttg
      361 gggaacgcgg cgttggcctg gtcccagtgc ttgcgcacca acgtccaggc ccgtgcaccg
      421 tggtgccggt tggcgatgca gcgtccgagc aggtacgggg cgttctgcga gcggaccgca
      481 ccgctgaacg cgaactcgca ggtccgctcg atcaacgcag cgtcgtcgaa ctcggccagc
      541 gcgtacaggt tgcgcaagct ctcttgcggt gtggtcgcgg cttcgaacgc ggcgacgaag
      601 cgttcgaagt cggtggcgtc gcccgtggcc gcgaccacgg tggtggcggc cgagatcagt
      661 tcgggatcgg ccgtgacccc attgccggag ctggtggcgc cgttggcatc gagcgcggcg
      721 agcaggtcgc gacaacgctg ctgcgcgtcg cgatcgcggc cgatcttggc caacgtggtc
      781 acgagtagtc cgcgcagttt cgagcgcagg tcgtcctcgt cgtcgctgnn nnnnnnnnnn
      841 nnnngcgcgg gtcgcaccag atcggccgcg atcgcggcga agcgttcttc acccgcgggg
      901 tcgagcaccc ggtgcaggcg accgagggca gcgacgatcg cctgccacac ggcgaggtcg
      961 cgctcgccgt cgaagccacg caggaactgg agcagacccg cggcgtcgag gcgtccggcg
     1021 acgaacgcag cgctggcgtc gtcgaccagg ttgtagcgct cgaccgtgcc gagggtggcc
     1081 agcgcggcgc cgttgaggcg gccgcgcagg gtgtcgtcgt aggcgacgcg gaagaagcct
     1141 tggccacccg cgttgacgac cacgacggcg tcaggatcgt cgatctcgat cttcgtctcg
     1201 gcgtcggtca gcagcacgta ctgttcgccg gttccgaccc gcaccgcaac gggcaccatg
     1261 aacagcgtcg ggtcgtcggc ggcggtggag aacgagaacc gctcctggcg gaggtgcagt
     1321 gacgagccac gcagcgacgc cgacaccagc gggtagccgg cctgccagat ccagctgtcc
     1381 atcatgtggc gcaccggctc gttgccgccg tcggcggtgg tgaccgcctc gatgccgtcc
     1441 cacagatcgc cggtctcggt gttgccgtac gagtgggtgc gcaggtagtg gttgacgccc
     1501 tggcggaacc gttcttcgcc gatgtactgc tgcagcatgc gcagcaatgc cccgcccttt
     1561 tgatacgtga gcacgtcgaa catgccctcg cactcggccg gggcgtgcac cgggaactcg
     1621 accgtgcggg tgctgctcag cgagtcgacc tcgaacgcat cgctgcgctc caggctgaag
     1681 ttggtccacc gctgccactg ggggcggaac gcgtcgacgg ccatgacttc catgaaggtg
     1741 gcaaaggctt cgttgagcca gatgccgttc caccagcgca tcgtgacgag gtcaccgaac
     1801 cac
//
LOCUS       DAORNF010000715         1802 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_47367_length_1802_cov_1.519748, whole genome shotgun sequence.
ACCESSION   DAORNF010000715 DAORNF010000000
VERSION     DAORNF010000715.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47367_length_1802_cov_1.519748"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="PLV68_20375"
     CDS             <1..318
                     /locus_tag="PLV68_20375"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRE03664.1"
                     /translation="STTCEPCKRELPAFAAAHNAFGDRVRFVGINSGGDTVAEAAAFA
                     EKYGVNYEMLKDPVGEFMAKLRVTSQPYTLFIAADGTIVEQKGLELSEATIRATITDT
                     LLS"
     gene            315..>731
                     /locus_tag="PLV68_20380"
     CDS             315..>731
                     /locus_tag="PLV68_20380"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HRE03665.1"
                     /translation="MIDGYFAYSFTLGLAATVNPCGFVLLPTYLMYYLGLEGNRPGTQ
                     RASLQRALLVSGAVSLGAVAVFVIVGAISHAFTSVIENNVKYAGFAIGLALIVLGCFL
                     LAGWKPRFALPQVNAGAERRQTFVSMLGFGVTYAVAS"
     assembly_gap    732..934
                     /estimated_length=203
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <935..1184
                     /locus_tag="PLV68_20385"
     CDS             <935..1184
                     /locus_tag="PLV68_20385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03666.1"
                     /translation="AALFVMATGFYLTWYWWAAITERAGSDGLTSRVERWNASISDLI
                     NRAGAWTMLVVLAVPIVAAVAVVLGGRRRRARGSGVGS"
     assembly_gap    1255..1414
                     /estimated_length=160
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1415..1789
                     /locus_tag="PLV68_20390"
     CDS             <1415..1789
                     /locus_tag="PLV68_20390"
                     /inference="COORDINATES: protein motif:HMM:NF017697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate hydrolase family protein"
                     /protein_id="HRE03667.1"
                     /translation="TMAGFLDYVDIVVTVHGFGRRGLFTTLLLGGRNRLLADHIGAAL
                     REALPDYDVCTDTDVIPVELRGLHPTNPVNLPRHHGVQIELPPRVRGSSPIWKDWTGP
                     GLTPHTESLITALAEAATTFSL"
BASE COUNT          203 a          496 c          470 g          270 t
ORIGIN      
        1 tccactacct gcgagccgtg caagcgggag ttgcccgcct tcgccgcggc gcacaacgcg
       61 ttcggcgatc gggtccgctt cgtcggcatc aactcgggcg gcgacacggt tgccgaggcc
      121 gccgcctttg ccgagaagta cggcgtcaac tacgagatgt tgaaggaccc ggtgggcgag
      181 ttcatggcca agctgcgcgt caccagtcag ccctacacgc tgttcatcgc ggccgacggc
      241 acgatcgtgg aacagaaggg tctcgagctc agcgaagcca cgatccgcgc gaccatcacc
      301 gacacgctcc tgtcgtgatc gacggctact tcgcgtacag cttcacgctc ggcctcgcgg
      361 ccaccgtcaa cccgtgcggc ttcgtgttgc tgcccacgta cttgatgtac tacctcggcc
      421 tggagggcaa tcggcccggt acccagcggg ccagtctgca gcgggcgctg ctggtcagtg
      481 gcgcggtatc gctcggcgcg gtggcggtgt tcgtcatcgt cggcgcgatc tcgcatgcct
      541 tcacctcggt gatcgagaac aacgtgaagt acgccgggtt cgcgatcggc ctcgccctga
      601 tcgtgctcgg ctgcttcttg ctcgcgggtt ggaaaccgcg cttcgccctg ccgcaggtca
      661 acgccggtgc cgagcggcgc cagacgttcg tgtcgatgct cggcttcggg gtcacctacg
      721 ccgtcgcgtc gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncgccgc gttgttcgtg atggccaccg
      961 gcttctacct cacgtggtac tggtgggccg cgatcaccga acgcgccggc agcgatggcc
     1021 tcaccagccg ggtcgagaga tggaacgcgt cgatcagcga cctgatcaac cgtgccgggg
     1081 cgtggacgat gctggtcgtg ctggcggtgc cgatcgtggc cgcggtcgcg gtcgtgctcg
     1141 gcggccgccg tcgtcgagcc cgcggatcgg gcgtgggtag ctgatgttgc gcgacctgct
     1201 cgctcgcccc ggggtgcgtg agcagtgtgt gcttcgctcg cggttcggct tcatnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnacgatg gccgggtttc tcgattacgt
     1441 cgacatcgtc gtcaccgtgc acgggttcgg tcgccgcggg ctgttcacca cgttgttgct
     1501 cggtggccgc aatcgcctgc tggccgatca catcggtgct gcgctgcgcg aggcactgcc
     1561 cgactacgac gtctgcaccg acaccgacgt gatcccggtc gaactgcgtg gcttgcaccc
     1621 caccaacccg gtgaaccttc ctcgccatca cggcgtccag atcgaactgc cgccgcgagt
     1681 ccgcggctcg agcccgatct ggaaggactg gacaggtccc ggtctcaccc cgcacaccga
     1741 gtccctcatc actgccctcg ccgaagccgc cacgacgttc tcactgtaag tgaccggccg
     1801 ct
//
LOCUS       DAORNF010000716         1801 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_47393_length_1801_cov_1.714204, whole genome shotgun sequence.
ACCESSION   DAORNF010000716 DAORNF010000000
VERSION     DAORNF010000716.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47393_length_1801_cov_1.714204"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /locus_tag="PLV68_20395"
     CDS             complement(<1..619)
                     /locus_tag="PLV68_20395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRE03668.1"
                     /translation="MPAPGMHSGVATDDAMNLSMSAKAVPLYEAVKRFIADEIEPVTE
                     EYFRRGEGRAERWGYGEGQLELLESVKSKAKAAGLWNFFLPDAETGEGLSNLDYAYIA
                     AELGRNPIASECFNCSAPDTGNMEVLERVGSPEQKKQWLNGEIRSAYVMTEPDVASSD
                     AKNIACRAVLDGDEWVINGEKYYASGAGDPRCKIMIVMVRTAEDGP"
     gene            complement(740..1609)
                     /locus_tag="PLV68_20400"
     CDS             complement(740..1609)
                     /locus_tag="PLV68_20400"
                     /inference="COORDINATES: protein motif:HMM:NF013143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HRE03669.1"
                     /translation="MSAATFATIMGTIDALQYDFMRRALIAALLVGLAAPAVGIFLVQ
                     RRLALIGDGLGHVAITGVALGLVLDRAPVTFALAAAIAGAVAIEVIRSRREAFAELAL
                     ALLFYGGLGGGIVLASRAPSGNRNLTQYLFGSITTTSASDVRTFAVLAAVVLVSSIGL
                     RRVLFTVSNDEEFAPVSASGTPVMAVNLALAVLSALTVVVSMRIVGVLLVSALMVVPV
                     AAAQMLARSFWQTFTASMVIGVGVAVCGVFVSFYADTPPGGTIVLLAIGVFALAFLGA
                     TAARSLLGAHRRR"
     gene            complement(1606..>1801)
                     /locus_tag="PLV68_20405"
     CDS             complement(1606..>1801)
                     /locus_tag="PLV68_20405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03670.1"
                     /translation="VVLHELGPIEPLVTRAVCLDGGAVVFDGPLAVAPAHLLHLGHDH
                     DPHGDSSAADPAGGIGLFGR"
BASE COUNT          314 a          627 c          602 g          258 t
ORIGIN      
        1 ccgggccgtc ttcggcggtg cggaccatga cgatcatgat cttgcaccgg ggatcgcccg
       61 caccggaggc gtagtacttc tcgccgttga tgacccactc gtcaccgtcg agcacagccc
      121 gacaggcaat gttcttggcg tcggagctgg ccacatcggg ttcggtcatc acgtatgccg
      181 aacgaatctc gccgttgagc cactgcttct tctgctcggg actgcccacc cgttcgagca
      241 cctccatgtt gccggtgtca ggagcgctgc agttgaagca ctccgacgcg atcgggttgc
      301 ggccgagctc ggctgcgatg taggcgtagt cgaggttgga caggccttcg ccggtctcgg
      361 cgtcgggcag gaagaagttc cacaacccgg ccgccttggc cttcgacttg accgattcga
      421 ggagttcgag ttggccctcg ccatagcccc accgctcggc ccggccctcg ccacggcgga
      481 agtactcctc ggtgaccggc tcgatctcgt cggcgatgaa ccgcttcacc gcctcgtaca
      541 gcggtaccgc cttggcagac atggacaggt tcatcgcgtc gtcggtcgcg acacctgagt
      601 gcatcccggg agcaggcatg gggttccctt tcgaagagtg gtcgccacat ccgcgaccgg
      661 gcaactgtgc tggttcggcc cgaccgtact gcagcggccg cgattcctcc tgatccgaag
      721 cggccaggac ccgtcgcgat cagcggcgcc ggtgtgcgcc cagcaggctg cgcgcggcgg
      781 tggcccccag gaaggcgagc gcgaacaccc cgatcgccag cagcacgatc gtgcctccgg
      841 gtggcgtgtc ggcgtagaag gagacgaaca ccccgcagac cgcgactccc acaccgatca
      901 ccatcgaggc cgtgaacgtc tgccagaagc tgcgcgccag catctgcgcc gcggcgaccg
      961 gcaccaccat caaggcgctg accagcagca cgccgacgat gcgcatcgac accacgaccg
     1021 tcaacgcgct gagcacggcc agagcgaggt tgaccgccat caccggcgtg ccgctggcgc
     1081 tgaccggcgc gaactcctcg tcgttgctga ccgtgaacag cacccgccgc agcccgatcg
     1141 acgacaccag caccaccgcg gcgagcacgg cgaaggtgcg cacgtcgctg gcggaggtgg
     1201 tcgtgatgct gccgaagagg tactgggtca ggttgcggtt acccgacggc gcccggctcg
     1261 ccaacacgat gccgccgccc aggccgccgt agaacagcag cgccagggca agctcggcga
     1321 aggcttcgcg gcgggaccgg atcacctcga tcgcgaccgc accggcgatc gcggccgcga
     1381 gcgcgaacgt gaccggtgcc cggtcgagca ccaaacccag cgcgacgccg gtgatcgcca
     1441 cgtggccgag gccgtcaccg atcagcgcca ggcggcgttg gacgaggaag atgccgacag
     1501 ccggagcggc caagccgacc agcagcgcgg cgatcagggc gcggcgcatg aagtcgtact
     1561 ggagcgcgtc gatcgtgccc atgatcgttg cgaaggtcgc tgcgctcatc gcccgaacag
     1621 accgattcca ccggcgggat cggcggcgga gctatcaccg tgggggtcgt gatcgtggcc
     1681 caagtgcagc aggtgagccg gggcgaccgc gagtggaccg tcgaacacca cggctccgcc
     1741 gtcgagacac acggcgcgcg tcaccaacgg ctcgatcggg ccgagttcgt gcaacacgac
     1801 g
//
LOCUS       DAORNF010000717         1800 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_47418_length_1800_cov_1.846991, whole genome shotgun sequence.
ACCESSION   DAORNF010000717 DAORNF010000000
VERSION     DAORNF010000717.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47418_length_1800_cov_1.846991"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="PLV68_20410"
     CDS             complement(<1..188)
                     /locus_tag="PLV68_20410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03671.1"
                     /translation="MPSIDEQSAAAVASRAADVLRPFGALARLDEVAVWAAAWNGVYR
                     IADGGASRAGDLIHPTSV"
     gene            complement(268..882)
                     /gene="cobO"
                     /locus_tag="PLV68_20415"
     CDS             complement(268..882)
                     /gene="cobO"
                     /locus_tag="PLV68_20415"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF004637.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HRE03672.1"
                     /translation="MDDSTGARETQQHDTRQHDDPPLDDPTAQGLRTASSLLLVNTGN
                     GKGKSSAAFGVMLRGVARGWNVAVLQFVKSGDWKVGEEEVGRQLGVHWQALGHGFTWD
                     SSNIDQDKQVARDAWAVARATIESGEHNLVILDELTYLMNWGWIDADEVIAVLRDRPP
                     KVNVIVTGRDASEALCAIADTVTEMREVKHAYRSGIRAKRGIDY"
     regulatory      937..1086
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            1172..>1800
                     /locus_tag="PLV68_20420"
     CDS             1172..>1800
                     /locus_tag="PLV68_20420"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRE03673.1"
                     /translation="MRRFVRTALSVAVTTLAVAACGSDGKAASSTTATSTTVATTSDA
                     GTGDASTTSSQAATPDSTGPGSTGSDATDGAVSVEAANGTISLPAPAAKIVSLSPTHT
                     EILFAIGAGDQVVAVDDQSNYPAAALDVATNLSGYTPNVEAIATYQPDLVVIGDDFNG
                     LTEQLGAVGITVWSGASPITLNDVYAQFEQLGVLTGHIGDAAEAVLGMQT"
BASE COUNT          272 a          621 c          589 g          318 t
ORIGIN      
        1 atcacgctag tcgggtggat cagatcgccg gcgcggctcg ccccgccatc ggcaatgcga
       61 tagacgccgt tccacgccgc ggcccacacc gcaacctcgt cgaggcgggc caacgcaccg
      121 aacggacgga gaacgtcggc ggcgcgtgac gcgaccgccg ctgccgactg ctcgtcgatg
      181 ctcggcatcg tcgccatcgc cgctcgcagc cacgctgtgg cgttgcctga tgggtcgccg
      241 acgccagtca cgtcacgctc agtcatgtca gtagtcgatg ccgcgcttgg ctcggattcc
      301 cgaccggtac gcgtgtttca cctcgcgcat ctcggtgacc gtgtcggcga tcgcgcacag
      361 cgcctcgctg gcatcgcggc cggtgacgat cacgttgacc ttcggcggtc ggtcgcgcag
      421 cacggcgatg acttcgtcgg cgtcgatcca tccccagttc atcaggtacg tcagctcgtc
      481 gaggatgacc aggttgtgct cgccagattc gatcgtggcg cgggcgacgg cccacgcatc
      541 gcgggcgacc tgcttgtcct ggtcgatgtt gctcgagtcc cacgtgaagc cgtggcccaa
      601 cgcctgccag tgcacgccca actggcggcc cacctcctcc tcgcccacct tccagtcgcc
      661 gctcttcacg aactgcaaca cggcgacgtt ccagccgcgg gccacgccgc gcagcatcac
      721 gccgaacgcg gctgagctct tgcccttgcc gttgccggtg ttgaccagca acagcgacga
      781 cgcggtgcgc aagccctgtg cggtcgggtc gtcgagtggc gggtcgtcgt gctggcgtgt
      841 gtcgtgttgc tgggtctcgc gggccccggt ggagtcgtcc atgcgccggt cagcctacgc
      901 aggccgtgcc tcatcagcgg gatgcgttgg tagggtcgct gtgcccggtt cgccgggtgc
      961 agcagttgca gcgaagccgg tgagattccg gcactgtccc gcaacggtga tgctggtgtc
     1021 gtgctggtcg ttcgatctgg cacggcggcc agacgagtcc ggacgcctgt ctctgttgga
     1081 ccgttcatcc ctcgaggcaa gggtggttcg gtgagcggct ccggctgtga acgaaccctg
     1141 atttgtcctc atcatcgatc gaggagttca catgcgcagg ttcgtccgga ccgcactgtc
     1201 cgtcgctgtc acaacccttg ccgtagcggc atgcggcagt gacggcaagg ccgcgagcag
     1261 caccactgcg accagcacga cggtcgccac gacgtctgat gctggcactg gcgacgccag
     1321 cacgaccagc agtcaggccg cgacccccga ttcgaccggt ccgggctcga ccggttcgga
     1381 cgcgaccgat ggagccgtct ccgtcgaagc tgccaacggc acgatctcgc tgccggctcc
     1441 ggcggcgaag atcgtctcgc tgtcaccgac gcacaccgag atcctgtttg cgatcggtgc
     1501 cggcgatcag gtggtcgccg tcgacgacca gtcgaactac cccgcggccg cgctggatgt
     1561 ggccaccaac ctcagcggct acacgccaaa cgtcgaggcg atcgccacct accagccgga
     1621 tctggtggtg atcggcgacg acttcaacgg gttgaccgaa cagctcggtg cggtgggcat
     1681 cacggtgtgg tcgggtgcct cgccgatcac cctcaacgac gtgtacgccc agttcgagca
     1741 gctcggcgtg ctgaccggcc acatcggcga tgccgccgaa gctgtgctcg gtatgcagac
//
LOCUS       DAORNF010000718         1800 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_47429_length_1800_cov_1.222350, whole genome shotgun sequence.
ACCESSION   DAORNF010000718 DAORNF010000000
VERSION     DAORNF010000718.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47429_length_1800_cov_1.222350"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            333..>858
                     /locus_tag="PLV68_20425"
     CDS             333..>858
                     /locus_tag="PLV68_20425"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRE03674.1"
                     /translation="MSPAGTVAHPPPIAWRELPIASPYLYETWLYARWPLPESVTGPV
                     DVVHATTLIPCPAKAPLVVTLHDLAFLHRPEQFTRHGNRIFRRSLEMIKRHADLVLCC
                     SQATVEDCVAAGIGAERLRHVPLGVDYQTATPADIRRVRSQYDLPERYLLFVGTVEPR
                     KNLRRLAEAVARLDE"
     assembly_gap    859..972
                     /estimated_length=114
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <973..1259
                     /locus_tag="PLV68_20430"
     CDS             <973..1259
                     /locus_tag="PLV68_20430"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRE03675.1"
                     /translation="VFCYPSVHEGYGLPVLEAMAQATPVVTSMATATEEAADGAAVLV
                     DPNDTDDIARGITEALARRAELAGRGKLRVAAATWQTTAEATAAAYRELV"
     assembly_gap    1417..1532
                     /estimated_length=116
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1533..>1800
                     /locus_tag="PLV68_20435"
     CDS             <1533..>1800
                     /locus_tag="PLV68_20435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03676.1"
                     /translation="LPGFAEAHPELAEWYPMVTARFSGRNRVRRVVGEHTWLARQVAG
                     SEIVHHGGGTVPGRPIRGSATVLTIHDLQFRSYPQYFSRIKLRY"
BASE COUNT          226 a          552 c          558 g          234 t
ORIGIN      
        1 gatcacggtg gcgcccgtgg cggccagttt ggcgacgacg acacggccga ggaacccgga
       61 accgccggtg acgaggacgg tggaagaggc gagcgggctg ggcatgaccc gttcatgcta
      121 cgtgcgccgg acgtgttgcc gagggcgggg gttcgcgtga tgaggccgcg tccgccatga
      181 tggacggtcc atgacgtctc ccggtaccgg cactgcgatc acggtcgcct acaccttgga
      241 gcagtgttgg caccgcgtac ctggtggaac cggtgtggct gcgctgcgca ccgcggcggc
      301 gatggcgcgc atcgacggcg tggagctgat cggtgtcgcc ggccggcacg gtcgcgcacc
      361 cgccgccgat cgcctggcgc gagctgccga tcgcgtcgcc gtatctctac gagacctggc
      421 tgtacgcccg ctggccgcta cccgaatcgg tcaccggccc ggtcgacgtc gtccacgcga
      481 ccacgctgat cccgtgcccg gccaaggcgc cactggtggt gacgctgcac gacctggcct
      541 tcctgcaccg ccccgagcag ttcacacgcc acggcaaccg catcttccgg cgcagcctgg
      601 agatgatcaa gcgccatgcc gatctggtgc tgtgctgctc gcaggccacc gtcgaggact
      661 gcgtcgcggc cggcatcggc gccgaacggc tgcgccacgt gcccctcggg gtcgactacc
      721 agaccgcgac cccggccgac atccgccgcg tccgctccca atacgacctg cccgagcggt
      781 atctgctgtt cgtcggcacc gtcgagccgc gcaagaacct gcgccgcttg gccgaggctg
      841 tcgcccgcct ggacgagcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      961 nnnnnnnnnn nnacgtgttc tgctacccca gcgtgcacga gggctacggg ctgccggtgc
     1021 tggaagcgat ggcccaggcg accccggtgg tgaccagcat ggcgacggcc accgaggagg
     1081 ctgccgacgg ggcagctgtg ttggtcgacc ccaacgacac cgacgacatc gcccgcggca
     1141 tcaccgaggc gctcgcgcgg cgggccgagt tggccggtcg gggcaagctg cgcgtcgctg
     1201 ctgccacgtg gcagaccacg gccgaggcga cggcggccgc gtaccgcgag ctggtctgaa
     1261 ccgcgccaga gccgcgccag gaacgaggcc gcttcgcggc tggagcagcc ggacgtcgtc
     1321 gtcacttcag cgagcgccac ggtcgcttcg gtaagttgtg cgcccatgtc gcctgattcg
     1381 atcccgatcg cgaccaggtc aggggcagcg gtgcggnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntgctgccc ggcttcgccg aggcgcaccc
     1561 agaattggct gagtggtacc cgatggtgac ggcccggttc agcggtcgca accgggtgcg
     1621 ccgcgtcgtt ggcgagcaca cgtggctggc gcgccaggtg gccgggtcgg aaatcgtcca
     1681 ccacggcggg ggcacggtgc cggggcgccc gatccgtggc tcggcgacgg tgctcaccat
     1741 ccatgatctg cagttccgca gctatcccca gtacttctcg cggatcaagc tgcgctactt
//
LOCUS       DAORNF010000719         1799 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_47446_length_1799_cov_1.704128, whole genome shotgun sequence.
ACCESSION   DAORNF010000719 DAORNF010000000
VERSION     DAORNF010000719.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47446_length_1799_cov_1.704128"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>215
                     /locus_tag="PLV68_20440"
     CDS             <1..>215
                     /locus_tag="PLV68_20440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020808074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03677.1"
                     /translation="LYRRVINRNNRLKRLLDLGAPEIIVNNEKRMLQEAVDALFDNGR
                     RGRPVTGPGNRPLKSLSDMLKGKQGRF"
     assembly_gap    216..481
                     /estimated_length=266
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <482..>1551
                     /locus_tag="PLV68_20445"
     CDS             <482..>1551
                     /locus_tag="PLV68_20445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HRE03678.1"
                     /translation="RLGIQAFEPVLVEGKALQIHPLVCSAFNADFDGDQMAIHVPLSA
                     EAQAEARVLMLSANNILSPASGRPTVTPSQDLIIGGYYLTEFVDDAPGAGRTFRHNWE
                     ILRALDEGSLDLHAPIHLRRPSGERYDSTPGRVLFEEALPADYPARFGHIDFLVKKNE
                     MGQIVERLSDNYNKSEVAGALDAIKAICYRYASQSGITVSISDVKTPKSKREILDRYE
                     AQAEKYEDQFSKGFITDGERRNMETQLWTRATGEVQDAMEAEFKAERFNPIDMMVGSG
                     ARGNMTQMRQIAGMRGLVANPRGDMIPRPIKKNFREGLEPLEYFIATPGARKGLVDTA
                     LRTADSGYLTRRLVDVSQELII"
     assembly_gap    1552..1593
                     /estimated_length=42
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1594..>1799
                     /locus_tag="PLV68_20450"
     CDS             <1594..>1799
                     /locus_tag="PLV68_20450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03679.1"
                     /translation="NVQPDTATTRSYLETKLFGRALLNPVTLSDGTVLAKNSLVGDDE
                     LVALRDDPNINRVRVRSVLTCDAD"
BASE COUNT          276 a          537 c          449 g          229 t
ORIGIN      
        1 cctgtaccgc cgcgtcatca accgcaacaa ccgcctcaag cgcctgctcg acctcggtgc
       61 ccccgagatc atcgtcaaca acgagaagcg gatgctgcaa gaagcggtcg acgcgttgtt
      121 cgacaacggt cgtcgcggcc gtccggtcac cggcccgggc aaccgcccgc tcaagtcgct
      181 gtccgacatg ctgaagggca agcagggccg cttccnnnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      481 nccgtctggg tatccaggcc ttcgagccgg tgctggtcga gggcaaggcg ctgcagatcc
      541 acccgctggt ctgctcggcc ttcaacgccg acttcgacgg cgaccagatg gccatccacg
      601 tgccgctgtc ggccgaggct caggccgaag cccgcgtgct gatgctgtcg gccaacaaca
      661 tcctgtcgcc cgcgtccggt cgcccgaccg tcacgcccag ccaggacttg atcatcggtg
      721 gctactacct gaccgagttc gtcgacgacg cgcccggcgc cggccgtacg ttccgccaca
      781 actgggagat cctccgtgcc ctcgacgagg gctcgctcga tctgcacgcc ccgatccacc
      841 tccgccgtcc ctcgggcgag cgctacgact cgacccccgg tcgcgtcctg ttcgaagagg
      901 ccctgccggc cgactacccg gcgcggttcg ggcacatcga cttcctcgtc aagaagaacg
      961 agatgggcca gatcgtcgag cgcctcagcg acaactacaa caagagcgaa gtcgccggtg
     1021 cgctcgatgc gatcaaggcg atctgctacc gctatgccag ccagtcgggc atcacggtgt
     1081 cgatcagcga cgtcaagacg ccgaagtcca agcgcgagat cctcgatcgc tacgaggccc
     1141 aggccgagaa gtacgaggac cagttcagca agggcttcat caccgacggc gagcgccgca
     1201 acatggagac ccagctgtgg actcgtgcca ccggcgaggt gcaggacgcg atggaggccg
     1261 agttcaaggc cgagcggttc aacccgatcg acatgatggt cggctccggt gcccgcggca
     1321 acatgaccca gatgcgccag atcgccggca tgcgtggcct ggtggccaac cctcgtggtg
     1381 acatgatccc ccgcccgatc aagaagaact tccgtgaggg cctcgaaccc cttgagtact
     1441 tcatcgccac accgggcgcc cgcaagggcc tggtcgacac cgcgttgcgt accgccgact
     1501 cgggctacct cacccgtcgt ctcgtcgacg tcagccagga gctgatcatc annnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnagaacgt gcagcccgac acggccacaa
     1621 cccgctcgta cctcgagacc aagttgttcg gccgtgccct gctcaacccg gtcaccctca
     1681 gcgacggcac cgtgttggcc aagaacagcc tggtcggcga cgacgagctg gtcgcgttgc
     1741 gggacgaccc gaacatcaac cgggtccgcg ttcgctcggt gctcacctgc gatgccgac
//
LOCUS       DAORNF010000720         1798 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_47484_length_1798_cov_1.580608, whole genome shotgun sequence.
ACCESSION   DAORNF010000720 DAORNF010000000
VERSION     DAORNF010000720.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1798
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47484_length_1798_cov_1.580608"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="PLV68_20455"
     CDS             <1..415
                     /locus_tag="PLV68_20455"
                     /inference="COORDINATES: protein motif:HMM:NF014841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRE03680.1"
                     /translation="VVEADRYVDCLAGADVVFLALSLTPETEGMIGRGELEMMEQHAW
                     LINVARGGHIVTDDLVWALSNGVIGGAGLDVTDPEPLPAGHPLWTLPNCIITPHVGNT
                     PEMAVPLLSERIATNVRRWARGEELIGLVDPQLGY"
     gene            433..1041
                     /locus_tag="PLV68_20460"
     CDS             433..1041
                     /locus_tag="PLV68_20460"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HRE03681.1"
                     /translation="MAHTVADHYAVLGVAATASRDEIRDAYRRLARQHHPDTGGEHAA
                     TRMAEINEAYRVLHDPARRALYDAARPLARGTAASSRSASPGSTSSTVHAQSSTHAAP
                     VPAPFPWKFLLALAGAGIAFVMINAALTDPPQPPRVDNILQIGSCVDILTNGDVGEVL
                     CSAAHDGVVTALRPVDGICAAPTALYRDRQGLGPVCVRPAHA"
     gene            1088..1160
                     /locus_tag="PLV68_20465"
     tRNA            1088..1160
                     /locus_tag="PLV68_20465"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1121..1123,aa:Val,seq:cac)
     assembly_gap    1390..1553
                     /estimated_length=164
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          246 a          553 c          563 g          272 t
ORIGIN      
        1 ggtggtcgag gccgaccggt acgtcgactg cctggcgggc gccgacgtgg tgttcctggc
       61 cctgtcgctc acacccgaga ccgaggggat gatcgggcgc ggcgagttgg agatgatgga
      121 gcagcatgca tggctgatca acgtcgcccg gggcggacac atcgtcaccg acgatctggt
      181 gtgggcgttg agcaacggcg tcatcggggg tgccggtctc gacgtcaccg accccgagcc
      241 gctgcctgcc ggccatccgc tgtggaccct gcccaactgc atcatcacgc ctcatgtcgg
      301 caacacgcct gagatggcgg tgccgttgct gtccgagcgg atcgcgacca acgtgcgccg
      361 ctgggcgcgg ggcgaagagc tgatcggcct cgtcgacccg cagctcggct actgacacgg
      421 acgagccggg tcgtggcaca cacggtggcc gaccactacg cagtcctcgg cgtcgcggcc
      481 acggcgagcc gggacgagat tcgcgacgcc taccgtcgtc ttgctcgcca gcatcatccc
      541 gacaccggtg gcgagcacgc cgccacccgg atggcagaga tcaacgaggc gtaccgggtg
      601 ctgcacgacc cggcccggcg ggcgttgtac gacgccgcgc gtccgctggc ccgcgggacg
      661 gcggcctcgt cacgatcagc gtcgccagga tcaacatcgt cgaccgttca cgcccagtcg
      721 tcgacccacg ccgcacccgt gccggcaccg tttccgtgga agttcctgct cgccctcgcc
      781 ggcgccggca tcgcgttcgt gatgatcaac gccgcgctga ccgacccacc gcagccaccg
      841 cgggtcgaca acatcctcca gatcggctcg tgcgtcgaca tcctgaccaa cggtgacgtc
      901 ggcgaggtgc tgtgcagcgc cgcccacgat ggcgtcgtca ccgccctgcg ccccgtcgac
      961 ggcatctgcg ccgcccccac cgccctgtac cgcgaccgcc agggtctcgg ccccgtgtgt
     1021 gtccgccccg cgcacgcctg acacgggtgg ggagcgagcg acccactgct agcatgcccc
     1081 acgcttcggg cgattagctc agttggtaga gcgctacgtt cacaccgtag aggtcgtcgg
     1141 ttcgagaccg gcatcgccca ccccaagcgc ctgagcacac gtgagcggtc ctgaatgcca
     1201 gcaaggccac accagaggca agtcgcgacg catgtgcgtg ggaagcaact cgccgtaccg
     1261 gtccgccaga tgttcgatcg tcttgtgtag atcgttgatg accgatcgtg ctgaccgttg
     1321 cgttctcatc tcgacgtcga acgcttcgtc gtcctcgacg gcctgagcga gccagacctt
     1381 tcgttgtcgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncgcctcc
     1561 cacgtctcgt cagggtggcg acgaggagat cgatatctga gtcccacgtg tcctcacccc
     1621 gcacgacaga gccgatgagg gacatcaggc tgcggttccg gttgtggcgg ctgctgccgc
     1681 ggttgggtgt tgggttgttt gggtgggggt ggggaagcag agtgcccagc ggtcgagtcg
     1741 ttcgccgagg gggtgggtgt aggcgggggt tggtccgtgg gggggggtga gtggcggg
//
LOCUS       DAORNF010000721         1796 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_47543_length_1796_cov_1.840322, whole genome shotgun sequence.
ACCESSION   DAORNF010000721 DAORNF010000000
VERSION     DAORNF010000721.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47543_length_1796_cov_1.840322"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..642)
                     /locus_tag="PLV68_20470"
     CDS             complement(<1..642)
                     /locus_tag="PLV68_20470"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRE03682.1"
                     /translation="MTAVPPPPATAIEWEPTEGPLASLRAGRGPRMVFAHGFTQTHRS
                     WLPVAEQFVVDHEVILVDMPGHGRSGHVRADLRLGADLLATVGGAAVYVGYSMGGRFV
                     LHLALAYPHLVRGMALLGATGGISNDDDRVERRIADEALADRIEAEGVEPFLNEWLAQ
                     PLFATLPPYARVLDDRITNTAEGLASSLRLAGTGTQLPLWDRVGWINEPTLIMA"
     assembly_gap    802..811
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<812..1311)
                     /locus_tag="PLV68_20475"
     CDS             complement(<812..1311)
                     /locus_tag="PLV68_20475"
                     /inference="COORDINATES: protein motif:HMM:NF013488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HRE03683.1"
                     /translation="MRIEIWSDVVCPWCYIGKRRFERALAAVAEQRDVSSFEIVYRPY
                     QLDPSADPDRTEPAADGYARKFGGAERAAALIANVTATAAEDGLEFRLDRAQRANTKR
                     AHRLLWFAEHQPDGIELQHRLKERLLQAYFTDGDDIGSLDTLVQAATEVGIDAEVTGR
                     GKHLWST"
     gene            1420..>1796
                     /locus_tag="PLV68_20480"
     CDS             1420..>1796
                     /locus_tag="PLV68_20480"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HRE03684.1"
                     /translation="MDASAVPADVAATFCATLVDEWVANGVTQAVIAPGSRSTVMALA
                     LAGDERLSVQVVHDERSAAFIALGVGLATGVPAVVVCTSGTAATHFYAAVVEAHLSAV
                     PMIVCTTDRPPELRDVGAPQTID"
BASE COUNT          253 a          587 c          639 g          307 t
ORIGIN      
        1 cgccatgatc aaggtcggtt cgttgatcca acccacccgg tcccacagcg gtagctgggt
       61 gccggtgccc gcgaggcgca ggctggaggc gagaccctcg gcggtgttgg tgatccgatc
      121 gtcgagcacg cgggcatagg ggggcagggt ggcgaacagc ggctgggcga gccattcgtt
      181 gagaaatggt tcgacccctt cggcctcgat ccggtcggcc agagcctcgt cggcgatgcg
      241 tcgctcgacg cgatcgtcgt cgttgctgat tccgccggtc gccccgagca gggccatgcc
      301 ccgcaccagg tgcgggtacg cgagggccag gtgcaacacg aaccggccac ccatcgagta
      361 gccgacatag accgctgcgc cgccgaccgt ggcgagcaga tcggcgccga ggcgaaggtc
      421 ggcgcggacg tgcccagacc gcccgtgacc gggcatgtcg accaggatca cctcgtgatc
      481 gaccacgaac tgttcggcca ccggcagcca cgaccgatgg gtctgggtga acccgtgggc
      541 gaacaccatg cgcggaccgc gtccggcgcg cagcgatgcc aacggcccct cagtgggctc
      601 ccactcgatc gccgtggccg gtgggggagg gaccgcggtc atgccgaact gcttgctgcc
      661 tcgttgtcga gcattttgtt gagcacccgt tcgaacgtgg cgctgtcctg ggcgcccggc
      721 accgcccact ggtcgttgaa cacgtaggtc ggcactgcgg tgatgccgtg gcgggcagcg
      781 atctggagca tctcgttgac cnnnnnnnnn ngtgctccag aggtgcttgc cgcggccggt
      841 gacctcggcg tcgatgccca cctcggtcgc ggcctgcacc agtgtgtcga gcgaaccgat
      901 gtcgtcgcca tcggtgaagt acgcctgcag caggcgttcc ttgagtcggt gctgcaactc
      961 gatgccgtcg ggctggtgct cggcaaacca caacaggcgg tgggcgcgct tggtgttggc
     1021 ccgctgggcg cggtcgagac ggaactccaa accgtcttcg gcggcggtgg ctgtgacgtt
     1081 ggcgatcagt gccgcggctc gctcggcacc gccgaacttg cgggcgtagc cgtcggcggc
     1141 tggttcggtg cggtcagggt cggccgaggg gtcgagctga tatgggcggt agacgatctc
     1201 gaagctgctg acgtcgcgct gttcggcaac ggccgccagt gctcgttcga accgtcgctt
     1261 gccgatgtag caccacgggc acaccacatc ggaccagatc tcgatgcgca tacccgcacc
     1321 ctactgccac ccgctctcgc cactcgccct tgacgtgtcc cgtgctggcc tcgcgtcggc
     1381 gtgggggtcc ggactcggtg tctggcacga tgggcggtga tggatgcttc cgctgtgcct
     1441 gctgatgttg cggcgacctt ttgtgcgacg ttggtggacg agtgggtggc gaacggggtg
     1501 acgcaggcgg taatcgcgcc ggggtcgcgt tcgacggtga tggcgctcgc gctggctggc
     1561 gacgagcggc tgagcgtgca ggtggtccac gacgagcgct ccgcggcctt catcgccctt
     1621 ggcgtcgggc tggccaccgg cgttccagcg gtcgtcgtgt gcaccagtgg caccgccgcc
     1681 acccacttct acgcggccgt ggtcgaagcg catctgtcgg cggtgccgat gatcgtctgc
     1741 accaccgacc gcccaccgga actgcgcgac gtgggcgccc cccagaccat cgacca
//
LOCUS       DAORNF010000722         1796 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_47548_length_1796_cov_1.458932, whole genome shotgun sequence.
ACCESSION   DAORNF010000722 DAORNF010000000
VERSION     DAORNF010000722.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47548_length_1796_cov_1.458932"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>210
                     /locus_tag="PLV68_20485"
     CDS             <1..>210
                     /locus_tag="PLV68_20485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03685.1"
                     /translation="MRRIHPEPTGPVTLAEAYDVDRPPPPGRPWVMLCMIASIDGSTA
                     VSGVSGGLGNEADRQLLTTLRGLADV"
     assembly_gap    211..365
                     /estimated_length=155
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <366..649
                     /locus_tag="PLV68_20490"
     CDS             <366..649
                     /locus_tag="PLV68_20490"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HRE03686.1"
                     /translation="ETVRAGRGEIDLTAALARLPGSPHLVQAEGGPLLNGALAAADLI
                     DEINLTLSPLVIGGDAERLTAHAPPVRHGFHLAHLIEHDSYLFARYVRA"
     gene            complement(708..1172)
                     /gene="nrdR"
                     /locus_tag="PLV68_20495"
     CDS             complement(708..1172)
                     /gene="nrdR"
                     /locus_tag="PLV68_20495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003762439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HRE03687.1"
                     /translation="MHCPSCRADDTKVVDSRLADEGCAIRRRRLCAQCSHRFTTYERV
                     EELPLMVVKSGATREPFDRAKVVSGVLAACKGRPVSSEQIHALAISVEDAARLHGNEV
                     TSAAVGAAVLDRLRELDEVAYLRFASVYKNFDAAGDFAEELQLLHKLQTPSH"
     gene            complement(1282..1707)
                     /locus_tag="PLV68_20500"
     CDS             complement(1282..1707)
                     /locus_tag="PLV68_20500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HRE03688.1"
                     /translation="MSTRAMSMTITSDVFEVRLGAVLHPNHRVYVRRRLLVGIAAAIL
                     ALVGVAAHEVLADRGGAPASTPAVLPASNVAVLGSATAVAALDGAVVGTPYLVQPGET
                     LWDIAKGWHGSQSFERYLDALIDQNGGASVQAGTLLLLP"
BASE COUNT          285 a          577 c          534 g          245 t
ORIGIN      
        1 atgcgccgca tccatccgga gccaaccggt cccgtcaccc tggccgaggc ctacgacgtc
       61 gaccggccgc cgcccccggg gcggccgtgg gtgatgttgt gcatgatcgc cagcatcgac
      121 ggctccaccg cggtgagcgg tgtgtcgggt ggtctcggca acgaggccga ccgccaactc
      181 ctgacgacct tgcgcggcct ggccgacgtg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnntcgaa accgtgcgcg ccggacgtgg cgagatcgat ctgaccgcag cactcgctcg
      421 cctccccggt tcaccccacc tggtccaagc agaaggtggg ccgctgctca acggcgcact
      481 cgccgcggcc gacctgatcg acgagatcaa cctgacgctg tccccactgg tgatcggtgg
      541 agacgccgaa cggctgacag cacacgcacc gcctgtgcgc cacggcttcc acctggcaca
      601 cctgatcgag cacgacagct acctcttcgc ccgctacgtg cgcgcctgat ccccacgccg
      661 caccccgcca acgcgtcagt gcgccgacac ctcagtgcgc cgacacctca gtgcgacggt
      721 gtctgcagct tgtgcaacaa ctgcagctct tcggcaaagt cgccagccgc gtcgaagttc
      781 ttgtacacac tggcaaagcg caggtaggcc acctcgtcga gttcgcggag acggtcgagc
      841 accgcggccc cgaccgcggc gctggtcacc tcgttgccgt gcagacgagc tgcgtcttcg
      901 accgagatcg ccagcgcgtg gatctgctcg ctggacaccg gccgcccctt gcaggccgcg
      961 agcactccgc tgaccacctt ggcccgatcg aacggctcgc gggtggcccc cgacttgacc
     1021 accatcagcg gcagctcttc gacgcgctcg taggtggtga agcgatgcga gcactgggcg
     1081 cacaggcggc gccggcggat cgcgcagcct tcgtcggcca ggcgcgagtc gacgaccttc
     1141 gtatcgtcgg cgcgacatga tgggcaatgc acggcgcaca ggctagtagc cacgattcgc
     1201 agtctttgtg cagatggaac gatctcgcgg gccacgatcg aacatgaggc gatcgaagcg
     1261 ccacggcccg gggcagacga atcagggcag cagcaacaag gtgcccgctt gcaccgacgc
     1321 gccaccgttc tgatcgatca acgcgtcgag gtagcgctcg aacgactgcg acccgtgcca
     1381 acccttggcg atgtcccaca gcgtctcacc cggctgtacg agatacggcg tgccgaccac
     1441 ggcgccgtcg agcgcagcga cagccgtggc cgatccaagg acggccacgt tcgacgccgg
     1501 caggaccgcg ggagtggagg caggagcacc cccgcggtct gccagcacct cgtgcgccgc
     1561 aacgcccacc agggccagga tcgcggcagc gatcccgacc aggaggcggc ggcgcacgta
     1621 gacccgatgg ttggggtgaa gcaccgcgcc gaggcgcact tcgaagacat ctgatgtgat
     1681 ggtcatcgac atggctcggg ttgacatggc agattcctgg tctctctgca tcgcggctgc
     1741 gacggtggtg gatgggatgg tcgtgtgcga tcacccgttc ggcgaacgag tgatcg
//
LOCUS       DAORNF010000723         1794 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_47617_length_1794_cov_0.868315, whole genome shotgun sequence.
ACCESSION   DAORNF010000723 DAORNF010000000
VERSION     DAORNF010000723.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47617_length_1794_cov_0.868315"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            44..>271
                     /locus_tag="PLV68_20505"
     CDS             44..>271
                     /locus_tag="PLV68_20505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03689.1"
                     /translation="MGGLNGFDFGQDGLLYGPLWFKGEIVAIDVAKAEMTTVARGFRI
                     PAAVNFNSTGTLYVVDTALGHFDPDGTRKVLA"
     assembly_gap    272..429
                     /estimated_length=158
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            444..>750
                     /locus_tag="PLV68_20510"
     CDS             444..>750
                     /locus_tag="PLV68_20510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03690.1"
                     /translation="MPADIAVASEGGKETVYVADTFSLRRVDGASGKVDEVVRTHGDI
                     HEYPLGVSLGAKNATMTSWFSGTAHVVERATGKTVRLVKDLKAPMDAIEHADGSLLVL
                     "
     assembly_gap    751..846
                     /estimated_length=96
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <847..1197
                     /locus_tag="PLV68_20515"
     CDS             <847..1197
                     /locus_tag="PLV68_20515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03691.1"
                     /translation="GGMLYVTEVGSGSVAQIDLASGTRKALAEGLLGPEGIDVGPDGR
                     LYVAEVGRAQVLAIDPRTGERVVVARNLSLGLPAVPGLPPSYVTTGVAVGASGAVYVS
                     SDVRNALYKLTPPQ"
     gene            1238..>1794
                     /locus_tag="PLV68_20520"
     CDS             1238..>1794
                     /locus_tag="PLV68_20520"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRE03692.1"
                     /translation="MAVVVPYVRDIKFEYGVAATVNPLVRRVVAKNPSAFTFHGTGTY
                     IVGKGNVAIVDPGPDDAAHVGALLDAVRGETVSHILITHTHMDHSPATAAVKKATGAT
                     VYGYGPHPRGEEIGAEGGDMDYAPDVVVTDGDVIEGKGWTSEAIHTPGHISNHLCFTL
                     REQNALFSGDHVMGWSTSVVTPPDG"
BASE COUNT          249 a          546 c          540 g          205 t
ORIGIN      
        1 cgacgtgaag ggcgaaaagc cgccgcgcaa gatcatggag aacatgggcg gcctcaacgg
       61 cttcgacttc ggccaggacg gcctgctgta cggcccgctg tggttcaagg gcgagatcgt
      121 cgccatcgac gtcgccaagg ccgagatgac gacggtcgcg cgcggcttca ggattcccgc
      181 cgccgtcaac ttcaactcca cgggcacgct ctacgtggtc gacaccgcgc tcggccattt
      241 cgatccggac gggacgcgga aagtgctcgc gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnna cggacgcctt gcgatgccgg ccgacatcgc cgtggcgagc gagggcggca
      481 aggagacggt gtacgtcgcc gacacgttct cgctgcgccg cgtcgacggc gcgtccggca
      541 aggtcgacga ggtcgtgcgc acgcacggcg acatccacga gtatccgctg ggcgtctcgc
      601 tgggcgcgaa gaacgcgacg atgacgagct ggttctcggg cacggcgcac gtggtcgagc
      661 gcgccacggg caagaccgtc cggctggtga aggacctgaa ggcgccgatg gacgcgatcg
      721 agcacgccga cggctcgctg ctggtgctgg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      841 nnnnnnggcg ggatgctgta cgtcaccgag gtcggctccg gcagcgtcgc gcagatcgac
      901 ctcgcgtcgg gaacgcgcaa ggcgctggcg gaaggcctgc tcggccccga agggatcgac
      961 gtcgggccgg acggccgcct ctacgtcgcg gaagtcgggc gcgcccaggt gctggcgatc
     1021 gacccgcgca ccggcgaacg ggtcgtcgtc gcccgcaacc tgtctctcgg cctcccggcg
     1081 gtccccgggt tgccgccctc ctacgtcacc accggggtcg ccgtgggcgc cagcggcgcg
     1141 gtgtacgtgt cctccgacgt ccgcaacgcg ctctacaagc tgaccccgcc gcaataagcc
     1201 ggcgcgccgc gcccgcccct ccaggagcaa gccgccgatg gccgtcgtcg tcccctacgt
     1261 gcgcgacatc aagttcgagt acggcgtcgc cgccaccgtc aatccgctgg tgcgccgcgt
     1321 cgtggccaag aacccgagcg cgttcacttt ccacggcacc ggcacctaca tcgtcggcaa
     1381 ggggaacgtc gccatcgtcg atccggggcc ggacgacgcc gcgcatgtcg gcgccctgct
     1441 cgacgcggtg cgcggcgaga ccgtgagcca catcctgatc acgcacacgc acatggacca
     1501 ttcgcccgcc accgccgcgg tgaagaaggc gaccggggcg accgtgtacg gctacggtcc
     1561 gcacccgcgc ggcgaggaga tcggcgccga gggcggcgac atggactacg cgcccgacgt
     1621 cgtggtgacg gacggcgacg tgatcgaggg caagggctgg accagcgagg cgatccacac
     1681 gcccggccat atctccaacc acctgtgctt cacgctgcgc gagcagaacg cgctgttcag
     1741 cggcgaccac gtgatgggct ggtcgaccag cgtcgtcacc ccgcccgacg gcga
//
LOCUS       DAORNF010000724         1792 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_47659_length_1792_cov_1.401842, whole genome shotgun sequence.
ACCESSION   DAORNF010000724 DAORNF010000000
VERSION     DAORNF010000724.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47659_length_1792_cov_1.401842"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1172)
                     /gene="drmB"
                     /locus_tag="PLV68_20525"
     CDS             complement(<1..1172)
                     /gene="drmB"
                     /locus_tag="PLV68_20525"
                     /inference="COORDINATES: protein motif:HMM:NF038324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DrmB family protein"
                     /protein_id="HRE03693.1"
                     /translation="MSTTKGTYNGPTRVGTIRPSQAIHSYGVGALIDLPNLSVMVGGL
                     DRWDVTRQAVITEERLLSAVQARLGAQVDRLLALPAEPQTTNNPYDDWARVGIPVSVF
                     PRWLRCTACNRLFPVASGALKLDYNAHRIDKTRYTHPNCDKARRPPPAVPARFVTGCT
                     NGHLDEFPWVQFAHDGVAVCANPILRVNDVGSGGRATDLQVACLTCGAKSTLAKAFGP
                     IAKTVMPQCRGRHPHLGQSSEPCNEQLRTLVLGASNLWFNASVSVLSLPVGGGSLAQL
                     VYDHWGAFEKMPVKEVLVYALASDRALAAAFAGEDPVEIWAAIEARRAGGTPATGTDV
                     RGPEWAALTKGSAGHTSPHFEAEPIAAPGAFSSKLAGVTLAHRLRVVTALCGFTRI"
     gene            complement(1169..>1792)
                     /locus_tag="PLV68_20530"
     CDS             complement(1169..>1792)
                     /locus_tag="PLV68_20530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03694.1"
                     /translation="KPRDLSHYETFEHYHQTYYRQVEALSVTPFARRALDRGLTALMV
                     AEARHSRPEWNPKPAAQTVPVHGADFDLIAASLRTRAQLVANNAAATAVDELVADRRD
                     KWSKQQAAPGVTLAYARGTGSEVNLLKSAEAGPWSVFTVPNSLREVEVGSNLLLREDD
                     PSDDPGAAFLPPPVASDADIDEVPQAAGDADPVDDAEAALEPDEVVL"
BASE COUNT          266 a          561 c          665 g          300 t
ORIGIN      
        1 ctgatgcggg tgaagccgca cagtgctgtg acgacacgca ggcggtgagc gagcgtgacc
       61 ccggcgagct tgctggagaa cgcccctggc gccgcgatcg gttcggcttc gaagtgcggc
      121 gaggtgtggc cagccgaccc cttcgtgagc gccgcccact cgggtcctcg cacgtcggta
      181 ccggttgccg gggttccgcc agcgcggcga gcctcgatcg ccgcccagat ctcgacaggg
      241 tcctccccgg caaacgcggc agcgagcgca cggtctgagg cgagcgcata gacgagcact
      301 tctttcacgg gcatcttctc gaacgcgccc cagtggtcgt agacgagctg cgcgagtgat
      361 ccgccgccaa ccgggagcga gagcaccgac actgacgcgt tgaaccacag gttcgaggcg
      421 ccgagcacga gggtgcggag ctgttcgttg cacggttcgc tcgattgccc gaggtgcggg
      481 tgtcggcctc ggcactgggg catcacggtc ttggcgatcg gcccgaaggc cttggcgagt
      541 gtcgacttgg ctccgcaggt caggcaagcg acctgcaggt cggtagcacg gccgccggag
      601 ccgacgtcgt tcacgcgcag gatcgggttc gcgcacacgg cgacgccgtc gtgggcgaac
      661 tgcacccagg ggaactcgtc gaggtggccg ttggtgcagc cggtgacgaa tcgtgcggga
      721 acggccggcg gtggacggcg tgccttgtcg cagttcgggt gggtgtaacg agtcttgtcg
      781 atccggtgtg cgttgtagtc gagctttaac gcgcctgacg cgacggggaa cagtcggttg
      841 catgcagtgc atcgcagcca acgagggaac accgagacgg ggatcccgac tcgcgcccaa
      901 tcgtcgtagg ggttgttcgt cgtctgcggc tcggccggca acgcgaggag ccggtcgacc
      961 tgagcgccga ggcgcgcttg cactgccgag aggagacgct cttcggtgat cacggcttgg
     1021 cgggtgacgt cccatcggtc gagcccaccg accatcacgc tcaggttcgg caggtcgatc
     1081 agggcaccca cgccgtagga gtggatggcc tggcttggcc ggatcgtgcc gacgcgggtc
     1141 gggccgttgt aggtgccctt cgtggtgctc acagcacgac ctcgtccggt tcgagtgccg
     1201 cttcggcatc gtcgacagga tcggcatcac ctgcggcctg cggcacctcg tcgatgtcgg
     1261 catcgctcgc tacgggtggc ggaaggaagg cggcgccggg gtcgtccgat gggtcgtcct
     1321 cgcggagcag caggttcgat ccgacctcga cctcgcgcaa ggagttgggg accgtgaaca
     1381 ccgaccacgg ccccgcctcg gcggacttca gcaggttgac ctcgctccct gtcccacggg
     1441 cgtaggcgag cgtgacaccc ggagcggctt gctgcttgga ccacttgtcg cggcgatcgg
     1501 cgacgagctc gtcgactgcg gtggcggcgg cgttgttggc aacgagctgg gcccgggtcc
     1561 gcagcgacgc cgcgatcaag tcgaagtcgg cgccatggac tggcacggtc tgcgcggcgg
     1621 gcttcgggtt ccactccggc ctggagtggc gggcctcggc caccatcagg gcggtgagcc
     1681 cgcggtccag cgctcgtcga gcgaacggtg tgacggacag tgcctcgacc tggcggtagt
     1741 aggtctggtg atagtgctcg aaggtctcgt agtgcgacag gtcgcggggc tt
//
LOCUS       DAORNF010000725         1791 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_47678_length_1791_cov_1.868088, whole genome shotgun sequence.
ACCESSION   DAORNF010000725 DAORNF010000000
VERSION     DAORNF010000725.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47678_length_1791_cov_1.868088"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="PLV68_20535"
     CDS             complement(<1..469)
                     /locus_tag="PLV68_20535"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HRE03695.1"
                     /translation="MKFGLMFSNTGMGSTAAGATDLAQRAEAAGIESLWTVEHTVVPS
                     GYQSAYPYDASGKMAGGAETFDLPDPLVWLAYVAAKTERIKLATGILILPQRNPLIAA
                     KEIATLDTMSGGRVLLGVGAGWLQEEFEAIGVPFTDRGQRLDDAIEALRALWST"
     gene            complement(505..1347)
                     /locus_tag="PLV68_20540"
     CDS             complement(505..1347)
                     /locus_tag="PLV68_20540"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HRE03696.1"
                     /translation="MDVEGRITEAGAALTTAERRVAEVVLSRPELVAFGTVAELAEAA
                     KAGAATVVRLAAKLGFDGFTALQAGVQAELSNQLRPAAVRIREARGGVAVQRHLEREL
                     DNVQATLLAVSESDLNAAARHLADLSASVLVLSGDASYGVSEQFVGDLSALRPGVELV
                     NSNPVAVARRLAQLSAGDVAVAIDLRRYDRWVVDATRQAKSRGAWVLSLSDSALSPLA
                     GMADVSMVLTAGGVGPFDSHVGTMALLNVLVAAAAELLTPTATDRLDRAEFLWSANGE
                     LIDR"
     gene            1489..>1791
                     /locus_tag="PLV68_20545"
     CDS             1489..>1791
                     /locus_tag="PLV68_20545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03697.1"
                     /translation="MPTTDLIRLAAEHAADLPAGVVAQVEAFRRTPPPAGCLLLRGMP
                     VGHLPPTPAHPTDASGLSPATEITLLSVASLLGEAVGYLPEHNGDLVQNIVPVPSAA"
BASE COUNT          263 a          647 c          604 g          277 t
ORIGIN      
        1 cggtgctcca cagcgcgcgc agcgcttcga tcgcgtcgtc gaggcgttgg ccacggtcgg
       61 tgaacggcac gccgatggcc tcgaactcct cttgcagcca ccctgccccg acgccgagca
      121 gcactcgccc accgctcatc gtgtcgagcg tggcgatctc cttggcagcg atcaacgggt
      181 tgcgctgggg gaggatgagg atgccggtgg caagtttgat ccgctcggtc ttggccgcca
      241 cgtaggccaa ccacaccagc gggtcgggca ggtcgaacgt ctccgctccc ccggccatct
      301 tgccgctggc gtcgtatggg taggccgact ggtagccgct cggcaccacg gtgtgctcga
      361 cggtccacag cgattcgatc ccggccgcct cggcccgttg ggccaggtcg gtggcgcccg
      421 ctgcggtgga ccccatgccg gtgttgctga acatcaagcc gaacttcatc tgcgcagcgt
      481 aggcatgccc ggcgctgcga gacgtcaacg gtcgatcagc tcgccgttgg cgctccagag
      541 gaactcggcc cgatcgaggc gatcggtggc cgtcggcgtc agcaattcgg ccgccgcggc
      601 gaccagcacg ttgagcagcg ccatggtgcc gacgtggctg tcgaacgggc cgacaccacc
      661 agcggtgagc accatcgaca cgtcggccat gcccgccagc ggcgacaggg cgctgtcgga
      721 cagcgagagc acccacgcgc cgcgcgactt ggcctgccgg gtggcgtcga ccacccaccg
      781 gtcgtaacga cgcagatcga tggcgacggc tacatcaccg gcgctgagct gcgccaggcg
      841 gcgggccacg gcgaccgggt tggagttcac cagctcgacc cctgggcgca gcgcggacag
      901 atcaccgacg aactgttcgc tgacgccgta agacgcgtcg ccactgagga cgagcaccga
      961 ggcggacaga tcggcgaggt ggcgcgccgc ggcgttgaga tcgctctcgg acaccgccaa
     1021 cagcgtggcc tgcacgttgt cgagctctcg ttcgaggtgg cgctgcacag cgacgcctcc
     1081 cctggcctcg cggatgcgca cggcggccgg gcgaagctgg ttcgacagct cggcctgcac
     1141 gccggcttgg agggcggtga acccgtcgaa gccgagcttg gcggccaggc gcaccaccgt
     1201 ggccgcaccc gccttggctg cttcggccag ctcggccacg gtgccgaagg cgaccagttc
     1261 gggacggctg agcacgactt cggccacgcg tcgttcagcg gtggtgagcg cggccccagc
     1321 ctccgtgatg cgtccctcca cgtccatggg cgccgagcgt agcgcttgca gctcagcacc
     1381 ccgccggtga cacccgttct ggcggtgaca ggattccgag atgcgcgtat acttggaaca
     1441 ggtgttccga cccgtatcgc tgtcgcctgt ttctgaggga ggttgctcgt gcccaccacc
     1501 gatctgatcc gcttggccgc tgaacacgcg gccgacctgc ctgccggcgt cgtcgcccag
     1561 gtcgaagcct tccgccgcac ccctccccca gcgggttgtc tgctgctgcg cggcatgccc
     1621 gtcggccacc tgccgcccac cccggcgcat ccgaccgacg cctcgggcct ctctccggcc
     1681 accgagatca ccctgctcag cgtcgcctcg ctgctcggcg aagccgttgg ctatctgccc
     1741 gagcacaacg gagacctggt gcagaacatc gtgccggtgc cgagcgcggc c
//
LOCUS       DAORNF010000726         1791 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_47679_length_1791_cov_1.834677, whole genome shotgun sequence.
ACCESSION   DAORNF010000726 DAORNF010000000
VERSION     DAORNF010000726.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47679_length_1791_cov_1.834677"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(62..1474)
                     /gene="lpdA"
                     /locus_tag="PLV68_20550"
     CDS             complement(62..1474)
                     /gene="lpdA"
                     /locus_tag="PLV68_20550"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HRE03698.1"
                     /translation="MAEQFDLVVIGGGPGGYGAAFYAASAGLHVAIVEKDTMGGTCLN
                     RGCIPAKAFLETAAVHRHVSHAAEFGIDTAAPSVNFAVAQARKQKIVDGLVKGINGLI
                     KMRKVALYSGTGALGPNRRVVVDQHDGQQVAIEGRAVILAAGSVPRTIPNFDRGGPIM
                     TSDEVLMLDAIPARVAVIGGGAIGCEFASTFADLGAQVTILEGLPKILPGLDTDVANV
                     VVRSFRKKNIDIKAGVMVTGHTPNGAGGTTVHFGPKDGPSENLDVDAVVVSVGRRPFA
                     DELGLVNTGVVVDERGFVQVDHFCRTGEAGVFAIGDLINTPQLAHVAYAEAILVIKQL
                     LNENPVPIAYDKVPWAIYCHPEVAFAGPDEEAAKALGYDVVVAKHQFRANSRAQIIGD
                     IDGLVKVIAKKNADGSAGQVLGVHMVGPWVTEQLSGGYLAVNWEATVAEVAEFIQPHP
                     SLSELFGETMLSLTGRSLNT"
BASE COUNT          284 a          601 c          607 g          299 t
ORIGIN      
        1 cttctctcaa tctcaggttt ctgtacggga gcgttcttgg gggctggtgg ggcgtgggac
       61 gtcaggtgtt gagactacga ccggtgagcg agagcatcgt ctcgccgaac aactcggaca
      121 agctcggatg gggctggatg aactccgcga cctcggccac cgtggcttcc cagttgacgg
      181 ccaggtagcc gccgctcaac tgctcggtga cccacgggcc gaccatgtgc acaccgagca
      241 cctgcccggc tgagccgtcg gcgttcttct tggcgatcac cttgaccaac ccgtcgatgt
      301 cgccgatgat ctgggcccga ctgttggccc ggaactggtg cttggccacg accacgtcgt
      361 agccgagcgc cttggccgcc tcttcgtcgg ggccggcgaa ggcgacctcg gggtggcagt
      421 agatcgccca cggcaccttg tcgtaggcga tcggcaccgg gttctcgttg agcagctgtt
      481 tgatcaccag gatcgcttcg gcgtacgcga catgggcgag ttgcggcgtg ttgatgaggt
      541 cgccgatggc gaacaccccc gcctcaccgg tgcggcagaa gtggtcgacc tgcacgaagc
      601 cacgctcgtc gaccaccaca ccggtgttga ccagcccgag ctcgtcggcg aacgggcgcc
      661 gcccgaccga cacgaccaca gcgtcgacgt cgaggttctc gctcggcccg tccttggggc
      721 cgaagtgcac agtggtaccg cccgcgccgt tcggcgtgtg gccggtgacc atgacaccag
      781 ccttgatgtc gatgttcttc ttgcggaagc tgcgcaccac cacgttggcg acgtcggtgt
      841 cgaggccagg caggatcttg ggcagaccct ccaggatcgt gacctgcgcc ccgagatcgg
      901 caaacgtcga tgcgaattcg caaccgatcg cgccaccacc gatgaccgcg acacgggcgg
      961 gaatggcatc gagcatcagc acttcgtcgc tggtcatgat cgggccgccg cgatcgaagt
     1021 tggggatcgt gcgcggcacc gagccggcgg ccaggatgac ggcccgcccc tcgatggcga
     1081 cctgctgacc gtcgtgctgg tcgacgacga cacgacggtt gggaccgagc gcgccggtac
     1141 cgctgtagag cgcaaccttg cgcatcttga tcaagccgtt gatgcccttg accaggccgt
     1201 cgacgatctt ctgcttgcgc gcctgagcga cggcgaagtt gacgctgggc gcggcggtgt
     1261 cgatgccgaa ctcggccgcg tggctgacgt ggcggtgcac ggccgctgtt tccaggaatg
     1321 ccttggcggg gatgcagccc cggttgaggc aggtgccccc catggtgtcc ttctcgacga
     1381 tcgcgacatg cagaccggcc gacgcggcgt agaacgcggc accgtacccg cccgggcctc
     1441 caccgatgac gaccagatcg aattgctcgg ccaccaggga ccacctccgt atttgtcgcg
     1501 ccaggttcgt gtcgcgacgg tgcgacgcga actgcgggtt tcaccgtagc gccactcgcc
     1561 atggcgagca cgaaagcgct cccgcgctgg cccggtcacc ggatggtgct gtcagcacca
     1621 cgatgtcaag cggcagccgg tcaggcgctg aggtcccaac gcagcacctg gcccgcgcgc
     1681 gcgtaggtga ggacaccggg ctggtcggaa gcgaaggtca ccgtgcagac gacctcggcc
     1741 gccgcggtgg tcgcaccgca ggcccggccc tcgcccgccg gcccggccgg g
//
LOCUS       DAORNF010000727         1778 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_48069_length_1778_cov_2.153221, whole genome shotgun sequence.
ACCESSION   DAORNF010000727 DAORNF010000000
VERSION     DAORNF010000727.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1778
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48069_length_1778_cov_2.153221"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="PLV68_20555"
     CDS             <1..327
                     /locus_tag="PLV68_20555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03699.1"
                     /translation="ALSVTFGQALRVTVMVELLAVSTGVGAQVSRARANLMTEDLFAW
                     AILLIAVVLVIELAILRPLTTHLLRWRSTPAETAATGRGATVAAAGVAQRPTTREMWA
                     AKASAK"
     gene            complement(276..1532)
                     /locus_tag="PLV68_20560"
     CDS             complement(276..1532)
                     /locus_tag="PLV68_20560"
                     /inference="COORDINATES: protein motif:HMM:NF019421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
                     /protein_id="HRE03700.1"
                     /translation="MIHDLATFIDGRRAGTLSQDRNGRLRYTYGADYPPDATPLSPRL
                     TVAGGDYTHADVAPFIAGLLPDNDQVLERWGRLFGVSPRNAFAILAHIGSDCAGAVQF
                     VPADGVDQLDGGALEPLTTADIAQMLRDLRADPYTWQQPAGPQAGHFSLAGAQTKFAL
                     HRASDGWARASGRIPTTHIFKPAIPGLAHHDLNEHLCLQAARRLGLLAASSEIAWFDD
                     ERVFVVERYDRRAVNGELRRLHQVDMCQALGVMPDLKYQRDGGPGPVEIITMIRSSLA
                     GEADVERFVQALVFNWLIGGTDAHAKNYGLVLSGTDARLAPLYDLASALVMPEWNWHK
                     WELAMRINKQGRFKYLGIEDWKRFATSAQIDRDVIDDAIDAFAPTLVDAVTDAARPLD
                     LGTDERSFVGHFADALAAHISRVVGR"
     gene            complement(1529..>1778)
                     /locus_tag="PLV68_20565"
     CDS             complement(1529..>1778)
                     /locus_tag="PLV68_20565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03701.1"
                     /translation="TPPPRARIDESMEALIHHFKIFTEGFKVPEGETYVSIESPRGEI
                     GIVLRVIAALDRELTISERASTGGDDLLGHVTGTGPGQ"
BASE COUNT          264 a          560 c          634 g          320 t
ORIGIN      
        1 gcgttgtcgg tcaccttcgg ccaggcgctg cgggtgacgg tgatggtcga gttgctcgcc
       61 gtgagcaccg gcgtgggtgc ccaggtgtcg cgggcgcggg ccaacctgat gaccgaagat
      121 ctcttcgcct gggcgatcct gctgatcgcc gtcgtgctcg tgatcgaact cgccatcctc
      181 cgccccctca ccacccacct cctccgctgg cgctccaccc ccgccgaaac ggccgcaacg
      241 gggcgtggcg cgacggtcgc cgctgctggc gtcgctcagc ggccgacgac gcgcgagatg
      301 tgggcggcca aggcgtcggc aaagtgaccg acgaaggatc gctcgtcggt gccgaggtcg
      361 agtgggcggg cagcatcggt gacggcatcg acgagtgtcg gcgcgaacgc gtcgatggcg
      421 tcgtcgatga cgtctcggtc gatctgcgct gacgtggcga accgcttcca gtcctcgatg
      481 ccgaggtact tgaaccggcc ctgcttgttg atgcgcatag cgagttccca cttgtgccag
      541 ttccactcgg gcatcacgag cgccgacgcg aggtcgtaga gcggtgccag gcgcgcgtcg
      601 gtgccgctca ggacgagtcc gtagttcttg gcgtgagcgt ctgtgccgcc gatcagccag
      661 ttgaacacga gtgcttggac gaaccgctcg acgtcggcct ccccagcgag cgacgaacgg
      721 atcatcgtga tgatctccac cgggccgggg ccgccgtcac gctgatactt gagatcgggc
      781 atcacaccga gggcttggca catgtcgacc tgatgcaagc ggcgcagttc gccgttgacg
      841 gcgcggcggt cgtagcgctc gaccacgaac acccgctcgt cgtcaaacca tgcgatctcc
      901 gacgaggcgg cgagcagacc gagacgtcgg gccgcttgca ggcacaggtg ctcgttgagg
      961 tcgtgatggg caaggccggg gatcgctggc ttgaagatgt gcgtggtcgg gatgcgtcct
     1021 gacgcgcgtg cccatccgtc gctggcgcga tgcagtgcga acttggtctg cgcgcccgcg
     1081 agcgagaagt gtcccgcctg tggtccggct ggctgttgcc aggtgtacgg gtcagcgcgc
     1141 aggtcgcgca gcatctgggc gatgtcggcg gtggtgagcg gttcgagcgc tcctccgtcg
     1201 agttggtcga cgccgtccgc tggcacgaac tgcaccgcac ccgcgcagtc agacccgatg
     1261 tgggcgagga tcgcgaacgc gttccgtggt gatacgccga acagccgccc ccagcgttcc
     1321 agcacctggt cgttgtctgg caagaggccc gcgatgaacg gtgccacgtc ggcgtgggtg
     1381 tagtccccgc ctgcaacggt cagccgtggt gagagcgggg ttgcgtctgg cgggtagtcg
     1441 gcgccgtagg tgtatcgcag acggccgttg cggtcttggg agagcgttcc ggcgcggcgt
     1501 ccatcgatga acgtggcaag gtcgtggatc actggccggg tcccgtgccg gtgacatggc
     1561 cgagcaggtc gtcgccacct gtgctggccc gctcgctgat cgtgagttcg cgatcaagtg
     1621 cagcgatgac gcgtagaacg atgccgatct cgccgcgagg actctcgatc gacacgtacg
     1681 tctcgccttc gggcaccttg aacccttcgg tgaagatctt gaagtggtgg atcagcgcct
     1741 ccatcgactc gtcgatgcgt gcccgcggcg gtggcgtg
//
LOCUS       DAORNF010000728         1775 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_48160_length_1775_cov_1.905814, whole genome shotgun sequence.
ACCESSION   DAORNF010000728 DAORNF010000000
VERSION     DAORNF010000728.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48160_length_1775_cov_1.905814"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(72..1490)
                     /gene="murC"
                     /locus_tag="PLV68_20570"
     CDS             complement(72..1490)
                     /gene="murC"
                     /locus_tag="PLV68_20570"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HRE03702.1"
                     /translation="MTGSGMPRPPLDLTRPQRLHVVGAGGPGMSAIAIVLAEMGHDTS
                     GSDLREQPILDRLRAGGVRINVGHTRDAVAGIDAITASSAIPASNIELDQARRDGATV
                     LRRAGMLASICAMAKSLAVAGTHGKTTTSSMLMLALAEAGWRPGFVIGGDVADVGTGA
                     QWTGSEWFVVEADESDGTHLELPLHGTILTNVEADHLDHYGTIDEIVAGFDRYLTHIP
                     GPKVLCGDNPVCADLAARHGAITYGLGAGNDFRAVELQPGRGSFGFVVERRGVRLGPV
                     TLPLRGVHNVTNATGVVAMAVSLGIDFDVVARALGRFGGVARRFDMRGEHGGVTFVDD
                     YGHLPTEIAATLAAARASDDKWQRVVAVFQPNRFNRMSVLSPEYRDAFVDADLVVLTD
                     IYASGTTPIPGVTGKLVVDAVLDAHPETQLVWLPRRGDLIDYLATHLRPGDVSISMGC
                     GDVASLPDEVIARRIEREHQRA"
     gene            complement(1496..>1775)
                     /locus_tag="PLV68_20575"
     CDS             complement(1496..>1775)
                     /locus_tag="PLV68_20575"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRE03703.1"
                     /translation="ELATVGAPAIVVPWAGAADDHQTDNAAVLGDVGGAIVRSEADLV
                     SRGLLGDVDALIGDPERLAAIGAAAFAAGDAHRSGRLTDLIERVAAAR"
BASE COUNT          271 a          634 c          607 g          263 t
ORIGIN      
        1 aacgtgctgg tgaacccgct gggcgagccc aagctgctcg acctgggcat cgccgaccac
       61 cggatcgagg ctcatgcccg ctggtgctct cgttcgatgc ggcgggcgat cacctcgtcg
      121 ggcaacgacg ccacgtcccc gcaacccatc gagatcgaca catcgccggg acggaggtgg
      181 gtggcgaggt agtcgatcag gtcgccccgg cggggcaacc acaccagctg cgtctcgggg
      241 tgggcgtcga gcaccgcgtc gaccaccagc ttgccggtga ccccggggat cggcgtggtg
      301 cccgaggcgt agatgtcggt cagcacgacg aggtcggcgt cgacgaacgc gtcgcgatac
      361 tcgggcgaca gcaccgacat gcggttgaac cggttcggct ggaacaccgc caccacccgc
      421 tgccacttgt cgtcgctggc gcgagcagcc gccagcgtgg ccgcgatctc ggtgggcagg
      481 tggccgtagt cgtcgacgaa ggtgaccccg ccgtgttcgc ctcgcatgtc gaatcggcgg
      541 gccacaccgc cgaagcgccc gagggcgcgg gcgaccacgt cgaagtcgat gccgagcgag
      601 acggccatcg cgaccacgcc cgtcgcgttg gtcacgttgt gcaccccgcg cagcggcagc
      661 gtgaccggcc ccagccgcac tccccgacgt tcgaccacga aaccaaagct gccccgtccc
      721 ggctgtagct cgacggcgcg gaagtcgttg cccgcgccga gtccataggt gatcgcgccg
      781 tggcgggctg ccagatcggc gcagaccggg ttgtcgccgc agagcacctt cggtccgggg
      841 atgtgggtga ggtagcggtc gaacccggcg acgatctcgt cgatcgtgcc gtaatggtcg
      901 aggtggtcgg cttcgacgtt ggtcaggatc gtgccgtgca gtggcaactc caggtgggtg
      961 ccgtcgctct cgtcggcttc gaccacgaac cactcgctgc cagtccactg cgcacccgtg
     1021 cccacgtcgg cgacgtcgcc gccgatgacg aaccccggcc gccacccggc ctcggcaagg
     1081 gccagcatca gcatgctcga ggtggtcgtc ttaccgtgcg ttccagcgac cgcgagcgac
     1141 ttggccatcg cacagatgct ggccagcatg cctgcccggc gcagcaccgt ggcaccgtca
     1201 cggcgagcct gatcgagttc gatgttcgat gccgggatcg ccgacgaagc cgtgatcgca
     1261 tcgataccgg cgaccgcatc acgggtgtga ccgacgttga tccgcactcc cccggcgcgc
     1321 agacgatcga ggatcggttg ctcgcgcaga tcgctgcccg acgtgtcgtg gcccatctcg
     1381 gccagcacga tcgcgatcgc ggacatcccc ggtcctccag ccccgacgac gtggagtcgc
     1441 tggggtcggg tgagatcgag cggcggtcgg ggcatgccag aacccgtcac cggtgtcatc
     1501 gtgccgccgc cacccgttcg atcaggtcgg tgagacggcc gctgcggtgg gcatcaccgg
     1561 ccgcgaacgc tgccgcaccg atggccgcca accgttccgg atcgccgatc agcgcgtcga
     1621 cgtcacccag caggccccgg ctgaccaggt cggcttcgct gcgcacgatc gcgccgccga
     1681 catcgcccag aaccgcggcg ttgtccgtct ggtggtcgtc ggccgcaccc gcccagggca
     1741 ccacgatcgc cggcgcgccc acggtggcca gttcg
//
LOCUS       DAORNF010000729         1767 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_48433_length_1767_cov_1.978388, whole genome shotgun sequence.
ACCESSION   DAORNF010000729 DAORNF010000000
VERSION     DAORNF010000729.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1767
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48433_length_1767_cov_1.978388"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(258..752)
                     /locus_tag="PLV68_20580"
     CDS             complement(258..752)
                     /locus_tag="PLV68_20580"
                     /inference="COORDINATES: protein motif:HMM:NF014373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HRE03704.1"
                     /translation="MVTAGLAWYEMVGRLGLALVLGAMIGAERELDGQDAGLRTHALL
                     SIGCALFALVSVAGFDQFVTDSTTNVNVDVTRVAAYVAGGVGFIGGGVIMKHRTRIAG
                     LTTAASLWTVAAIGVACGIGFWVGALAATVLSVAVLGPLERISRRVRARSRRSDPDGD
                     PVGG"
     gene            899..1675
                     /locus_tag="PLV68_20585"
     CDS             899..1675
                     /locus_tag="PLV68_20585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HemK family protein methyltransferase"
                     /protein_id="HRE03705.1"
                     /translation="MAATLDHERVRAQLEAAGCITADAEALELRRAAPDDATLRSWIQ
                     RRMDGEPLAWITGSTTFCGRRILIDPGVYVPRWQTEALARRAADVLPRDGGRAVDLCT
                     GSGAVAAHLAAEAPDAIVIATDLDVRSARCARRNGVIAIVADLGEPIRSTSADVVTAV
                     APYVPTASIDLLPSDVRPFEPRLALDGGRDGLDIVHRAAITAARLLRPAGWFLTEIGG
                     DQADILHDVLVRLGFGEIDPWYDDEGDLRGIAAQTTHRTS"
BASE COUNT          301 a          646 c          562 g          258 t
ORIGIN      
        1 cgtcgaccag cgccgtgaag tagatgtggt tcatcacgat ctcaggccga tagttcgacc
       61 ggtgcccagc ggtgagcaag tcacccacgc gcaggtccgc gaccgtgccg tgaaagaacg
      121 gcccttcctc aagaacgctg ctcatcgacg tccctcctcc gcgatctcag ctcgctccgc
      181 tccaccaaca ccatcacccc aaccccatgc gccgggggca cgaccctaac ggccgaaaca
      241 acggctgcgc gatccggtca gccgccgact gggtcaccat cggggtcgct gcggcggctg
      301 cgagctcgga cgcggcggct gatccgctcc agcggcccca acaccgccac cgacagcacc
      361 gtcgcggcga gcgccccgac ccagaagccg atgccgcatg ccaccccgat cgcagccacc
      421 gtccacagcg acgccgccgt cgtcaacccg gcgatgcggg tgcggtgctt catgatcacg
      481 ccaccaccga tgaagcccac gccccctgcc acgtaggccg cgacgcgggt gacgtcgacg
      541 ttcacgttgg tggtcgagtc ggtgacgaac tggtcgaacc cggcaacaga gaccagagcg
      601 aacagggcac acccgatcga gagcaaggcg tgcgtgcgca gccctgcgtc ctgaccgtcg
      661 agctcgcgct cggctccgat cattgctccc agcacgaggg cgagcccgag acgcccgacc
      721 atctcgtacc aggccagccc ggccgtcacc atcgccggcc caacgccatc cccaacaccg
      781 cgtgctccac ggtgttgtgg ttaccccaac ccacccccgg tgaaaccgtg ctcgccgaat
      841 cttcttcccc gaactgtgct ggcgaagggt cacgcttcgt gcgcggcaga ctcggggcat
      901 ggcggcgacg ctcgaccatg agagagttcg ggcacagctc gaggctgcag ggtgcatcac
      961 cgcggatgcc gaggcgttgg agctgcggcg cgcggcaccc gacgatgcaa ccctccggtc
     1021 gtggattcag cgccgcatgg acggtgaacc gttggcgtgg atcaccggct cgaccacgtt
     1081 ctgcgggcgc cggatcctga tcgacccggg cgtgtacgtg ccgaggtggc agaccgaagc
     1141 gttggcgcgc cgcgctgcag acgtgctccc gcgggacggg ggtcgtgccg tcgacctctg
     1201 taccggctct ggagcggtcg ccgcccatct cgccgccgaa gcgccggatg caatcgtgat
     1261 cgcgaccgat ctcgacgtca ggtcggctcg gtgtgcgcgt cggaacgggg tcatcgcgat
     1321 cgtggccgat ctcggcgagc cgatccggag cacgtcggcc gacgtcgtga cagccgtcgc
     1381 accgtatgtg ccgaccgcca gcatcgacct gctcccttcc gacgtgcgcc ccttcgagcc
     1441 acgcttggcg ctcgacggag gccgcgacgg gctcgacatc gtgcaccggg cagccatcac
     1501 cgcagcgcgc ttgcttcggc cggccggttg gttcctcacc gagatcggcg gtgaccaggc
     1561 agacatcctg cacgacgtgc tcgtgcgact cggcttcgga gagatcgacc cgtggtacga
     1621 cgacgaaggc gacctccgcg gcatcgcagc gcaaaccacc caccgcacca gttagggagc
     1681 gggatgtgca cgacctcgac ggctgaggca acaccggtac caacatgttg agtgaaccgc
     1741 cccgggatcg gtgactgctc gttctct
//
LOCUS       DAORNF010000730         1767 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_48443_length_1767_cov_1.481893, whole genome shotgun sequence.
ACCESSION   DAORNF010000730 DAORNF010000000
VERSION     DAORNF010000730.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1767
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48443_length_1767_cov_1.481893"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..728
                     /locus_tag="PLV68_20590"
     CDS             <1..728
                     /locus_tag="PLV68_20590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRE03706.1"
                     /translation="SLDPKMTVGASIAEPLAIQRVKTDPDERVAELLELVGLAPDHAR
                     RFPHEFSGGQRQRVGVARALALNPVVLDEPVSALDVSIQAGVVNLLQDLQNQLGLSYL
                     FIAHDLSVVRHISDVVAVMYLGKLMEIAPANQLYEAPAHPYTKALLSAVPFPDPETER
                     ARQRIMLQGDPPSPIAPPSGCRFRTRCQYAEEICATDAPVLEEITAGHQVACHFPHIT
                     ASVTISGSGISSARISGTEAADR"
     gene            740..982
                     /gene="secG"
                     /locus_tag="PLV68_20595"
     CDS             740..982
                     /gene="secG"
                     /locus_tag="PLV68_20595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HRE03707.1"
                     /translation="MRDLFLYVSLVLNVASLIGMVAGILMHSGRGGGLSDMFGGGGGA
                     ALGSTSAERNLTRITLVFTLIWALTVIALGFLLVRQ"
     gene            1012..1095
                     /locus_tag="PLV68_20600"
     tRNA            1012..1095
                     /locus_tag="PLV68_20600"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1046..1048,aa:Leu,seq:gag)
     assembly_gap    1308..1417
                     /estimated_length=110
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1564..>1767)
                     /locus_tag="PLV68_20605"
     CDS             complement(1564..>1767)
                     /locus_tag="PLV68_20605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03708.1"
                     /translation="IDDVTLDDLVNEARSWGLTPRAAHQTVSRTIDSIHEALQHTQPT
                     VVDLHQLIAHRLRHLTPNRPQFR"
BASE COUNT          247 a          521 c          574 g          315 t
ORIGIN      
        1 cgtcgctcga tccgaagatg accgtcggcg catcgatcgc cgagccgctc gcgatccagc
       61 gggtcaagac cgacccggac gagcgcgtcg ccgagttgct cgaactggtc ggcctcgcgc
      121 cggaccacgc gcggcggttc ccgcacgagt tctccggcgg ccagcgccag cgggtcggtg
      181 tggcccgggc gttggcgctg aaccccgtgg tgctcgacga gcccgtgtcc gcgctcgacg
      241 tcagcattca ggccggggtg gtcaacctgt tgcaggacct gcagaaccaa ctcggcctga
      301 gctacttgtt catcgcgcat gacctgtcgg tggtgcgcca catcagcgac gtcgtggccg
      361 tgatgtatct cggcaagctg atggagatcg ccccggcgaa tcagttgtac gaggcgccgg
      421 cgcatccgta caccaaggcg ctgctctcgg ccgttccgtt tcccgacccc gagaccgagc
      481 gggcgcggca gcggatcatg ttgcagggcg atccgccgag cccgatcgcc ccgcccagcg
      541 gctgccggtt ccgcacgcgc tgccagtacg ccgaggagat ctgtgccacc gacgcccccg
      601 tgttggagga gatcaccgca ggccaccagg tggcgtgcca cttcccccac atcaccgcga
      661 gcgtcacgat cagtggctcc ggtatcagca gcgctcgtat cagtggcacc gaagctgccg
      721 accgctagga tttcgccccg tgcgtgacct gtttctctac gtcagcctcg tcctcaacgt
      781 ggcgtccctg atcgggatgg tcgccggcat cctcatgcac agcggtcgcg gcggcggtct
      841 gtccgacatg ttcggcggcg gcggtggcgc ggccctcggt tccacgtccg ccgagcgcaa
      901 cctcacccgc atcacgctgg tcttcacgct gatctgggcg ttgacggtca ttgcgctcgg
      961 gttcctgttg gtacgccagt gaggctccgc taacctcggt agtccaccca cgtcgaagtg
     1021 gcggaatagg tatacgcgct agcttgaggt gctagtggga gaaatcccgt gggggttcaa
     1081 gtcccccctt cgacactggg ctgagccgtc aggctcggcg gcgttcgata gcatgacggc
     1141 atggctcgac gtgctcgcga tgtcttacgt gatccagtcg tcgtactcat tgaggaaccg
     1201 accggggatg tcgatgagtg gttgaagtcg ttgaggtggg caacgcccct cgatctcaag
     1261 gtcacagcgg ctgagctact cgccgaggcg cgggccgaag aggagtgnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnggg acgtgccgct cacgagcggc
     1441 gacatcctcc gatcggaccg tttgaccggc ggcccgcaca ccgcgtctga tccgacgtag
     1501 tccacgagcg ctgcgccgtt gatgagcgtg tcggcgagcc gctgtctcgc ctgcgcgtgt
     1561 cgcttaccgg aattgggggc ggttcggtgt cagatggcgg agtcggtgtg cgatgagttg
     1621 atgaaggtcc acgacggtgg gctgggtgtg ttggagggct tcgtggatgg agtcgatggt
     1681 gcgactgacg gtttggtgtg cggcgcgtgg ggtgaggccc catgatcgtg cttcgttgac
     1741 gaggtcgtcg agggtgacgt cgtcgat
//
LOCUS       DAORNF010000731         1763 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_48569_length_1763_cov_1.850117, whole genome shotgun sequence.
ACCESSION   DAORNF010000731 DAORNF010000000
VERSION     DAORNF010000731.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1763
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48569_length_1763_cov_1.850117"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            169..879
                     /locus_tag="PLV68_20610"
     CDS             169..879
                     /locus_tag="PLV68_20610"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRE03709.1"
                     /translation="MELILVRHGQPAWSDPDGFGRNDPELTELGRRQAELAARRLANS
                     EELPGRGDIDRVFASPAVRAQQTAAPIAAALGLEIVTLDWLLEVQNPPEWEGSPMANI
                     DAAFQTMYGQPRETWWAGHPGGENFASFHERIRAGITATLAELGITPSGERGLWDVAE
                     IEAGLDRVVMVAHGGTNGAVVANLLQVEPEPWEWERFRNGHAAFSVLHSLPIAGHHIW
                     SLDQVGDANHLPVADRTF"
     gene            961..>1763
                     /locus_tag="PLV68_20615"
     CDS             961..>1763
                     /locus_tag="PLV68_20615"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HRE03710.1"
                     /translation="MVIRAWRVLAALNVLALTAAAAVVLIVGGAQPGVVRAADPLRVV
                     AWAPYWEKTSSLNSFTANQAVMSELTPMFWYASGATGTISRYASATAADVTKYRNAAT
                     AAGKPIVATVFDGMSARGMSNLLSTAQGRTAHVENIMSFVTINGFDGIDIDYEQFAFA
                     DGRATWPATYTNWGLFLEELGGRLHALGKTLSVAVPPVYDAGQTSASGYWVYNYAAVG
                     QFADNVRIMTYSYSGNAAGVISPYWWVEQSMRAAAAIVPPEKIQMGIAS"
BASE COUNT          269 a          591 c          619 g          284 t
ORIGIN      
        1 gctgcagggt cgttgatccg cacgcacaac tgacggttac ccgacgctgg ccgtgcccct
       61 ggtggtacgg ccagcggacg tttttcggtc gggggcctcg gagagccggc gcgggcgtga
      121 cgaacctgct ggtcggccga ccgtgccccg tcggtagggt cgggccacat ggagttgatc
      181 ctcgttcgcc atggccagcc cgcgtggtcc gaccctgatg ggttcggtcg caacgacccc
      241 gaactgaccg agctcggtcg tcgccaggcc gagctcgccg ctcggcgcct ggccaactcc
      301 gaagagctgc ctggccgggg tgacatcgac cgcgtcttcg cgtcgcccgc agtgcgggcc
      361 caacagacgg cggcgccgat cgcggccgcg ctgggactgg agatcgtcac gctcgactgg
      421 ctgttggagg tccagaaccc acccgaatgg gaagggtcgc cgatggccaa catcgacgcc
      481 gcgttccaga cgatgtacgg ccaaccccgc gagacatggt gggcggggca tcccggtggc
      541 gagaacttcg ccagcttcca cgaacgcatc cgcgccggca tcacggccac gctggccgag
      601 ttgggcatca ccccgtcggg cgagcgtggc ttgtgggacg tcgccgagat cgaggccggc
      661 ctcgaccggg tggtgatggt ggcccacggc ggcaccaacg gagcggtggt cgccaacttg
      721 ttgcaggtgg agcccgaacc gtgggagtgg gagcggttcc gcaacggcca tgccgccttc
      781 tccgtgctgc actcgttgcc catcgccgga caccacatct ggtcgctcga ccaggtcggc
      841 gacgccaacc atctccccgt tgccgaccgc acgttctgat cggctggtgc cgatctcggg
      901 ttcaccccga ccgcgcgcgg ccgggcagcc gtcctagggt gtctggccga tggaggctcg
      961 atggtgatcc gggcgtggcg ggtactcgcc gcgctgaacg tactggccct gactgcggct
     1021 gctgccgtgg tgctgatcgt gggcggcgcc caacccggcg tggtgcgcgc ggccgacccg
     1081 ctgcgggtgg tggcgtgggc accgtattgg gagaagacct cgtcgttgaa ctcgttcacc
     1141 gccaaccagg cggtgatgag cgagttgacg ccgatgttct ggtacgccag cggcgcaacg
     1201 ggcacgatca gccgctacgc gtcggccacc gcggccgacg tcaccaagta ccgcaacgcc
     1261 gcgaccgcgg cgggtaagcc gatcgtcgcc acggtgttcg acggcatgtc cgcccgcggc
     1321 atgtcgaacc tgctgtccac cgcgcagggg cgtacagcgc acgtcgagaa catcatgtcg
     1381 ttcgtcacca tcaacgggtt cgacggcatc gacatcgact acgagcagtt cgcctttgcc
     1441 gacgggcggg ccacgtggcc agcgacgtac accaactggg gcctgttcct cgaagagctc
     1501 ggcgggcggt tgcatgcgct cggcaagacg ctgtcggtcg cggtgccgcc ggtgtacgac
     1561 gcgggccaga cgtcggccag cggctattgg gtctacaact acgccgccgt cggtcagttc
     1621 gccgacaacg tgcggatcat gacctactcg tacagcggca atgccgcggg tgtgatcagc
     1681 ccgtactggt gggtcgagca gagcatgcgc gccgcggcgg cgatcgtccc acctgaaaag
     1741 atccagatgg gcatcgcgtc gta
//
LOCUS       DAORNF010000732         1762 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_48593_length_1762_cov_1.685413, whole genome shotgun sequence.
ACCESSION   DAORNF010000732 DAORNF010000000
VERSION     DAORNF010000732.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48593_length_1762_cov_1.685413"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1062)
                     /gene="ffh"
                     /locus_tag="PLV68_20620"
     CDS             complement(<1..1062)
                     /gene="ffh"
                     /locus_tag="PLV68_20620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HRE03711.1"
                     /translation="MFDTLSDRFDGIFKRLRGKGRLTDRDVDEVLREIRTALLEADVN
                     VSVVRTVVDRIRDAAVGAKLSQVLDPGQQVVKIVNNELTAILGGETLKITYAGRPPTV
                     VLMAGLQGSGKTTNSAKLARWFKSQGRQPLMVGADLQRPAAVEQLRTLGRQIDVPVFS
                     EASDPVTVATRGLAEARRLGRDVLIVDTAGRLSVDTEMMEQVRQINDAIQPNYTFLVI
                     DAMTGQDAVGVAEAFHQTLAIDGVILSKLDGDARGGAALSVKEVIGRPIAFASTGEKL
                     DAFEQFHPDRMAGRILGMGDVLTLIEQAEKTFEKEAAEEAAERLMAGEFTLDDFLEQM
                     QQLKKMGPLSGLLGMMPGMP"
     gene            complement(1083..>1389)
                     /locus_tag="PLV68_20625"
     CDS             complement(1083..>1389)
                     /locus_tag="PLV68_20625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HRE03712.1"
                     /translation="MEELRKVRRVASKGKGRVTETLLVIDATTGQNGLAQAREFGNTT
                     EVTGVVLTKLDGSAKGGIVFAIETELGLPVKLVGVGETIDDLVAFDPDEFIAALFAE"
     assembly_gap    1390..1507
                     /estimated_length=118
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1508..>1762)
                     /locus_tag="PLV68_20630"
     CDS             complement(<1508..>1762)
                     /locus_tag="PLV68_20630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HRE03713.1"
                     /translation="QAEMVARLAGADRTLHFEPGEPDSPNVWLFVGVNGVGKTTTIGK
                     VGAAQIESGRSVLMAAGDTFRAAAAEQLGTWAERSGADIV"
BASE COUNT          253 a          546 c          561 g          284 t
ORIGIN      
        1 gggcatgcct ggcatcatgc ccagcagccc cgacagcgga cccatcttct tgagctgctg
       61 catctgttcg aggaagtcgt cgagggtgaa ctcccccgcc atcaaccgct cggccgcctc
      121 ttcggcggcc tccttttcga aagtcttctc ggcctgctcg atcagggtga gcacgtcgcc
      181 catgccgagg atgcgccccg ccatgcgatc gggatggaac tgctcgaacg cgtcgagctt
      241 ctcaccggtg gacgcgaacg cgatcgggcg accgatgacc tccttgacgc tgagggcggc
      301 accaccgcgg gcgtcgccgt cgagcttgga caggatcaca ccgtcgatgg ccagcgtctg
      361 gtggaacgcc tcggcgacgc cgacggcgtc ttgaccggtc atcgcgtcga tgaccaggaa
      421 cgtgtagttg ggctggatcg cgtcgttgat ctgacggacc tgctccatca tctcggtatc
      481 gaccgacagg cgtccggcgg tgtcgacgat gagcacgtca cggccgaggc ggcgcgcctc
      541 ggccaggccg cgggtggcga cggtgaccgg gtcgctggcc tcgctgaaca ccggcacgtc
      601 gatctggcgg cccagggtgc gcaactgctc gaccgcggcg ggacgctgca ggtcggcacc
      661 gaccatcaac ggttgacggc cctggctctt gaaccagcga gcgagcttgg ccgagttggt
      721 ggtcttaccc gaaccctgca gaccggccat gagcaccacg gtgggcgggc gaccggcgta
      781 ggtgatcttg agcgtctcgc caccgaggat ggccgtgagc tcgttgttga cgatcttgac
      841 gacctgttgg ccgggatcga gcacctgtga caacttggcg ccgaccgccg cgtcgcggat
      901 gcggtcgacg acggtgcgga ccaccgagac gttgacgtcg gcttcgagca gtgctgtgcg
      961 gatctcacgc agcacctcgt cgacgtcacg atcggtgagt cgacccttgc cacggaggcg
     1021 tttgaagatg ccgtcgaagc ggtcggagag tgtgtcgaac atggtgccga caggctatgt
     1081 catcactccg cgaacagcgc ggcgatgaac tcgtcggggt cgaacgcgac caggtcgtcg
     1141 atcgtctcgc cgacgccgac cagtttcacc ggcaggccga gctcggtctc gatcgcgaac
     1201 acgatgccac ccttggccga cccgtcgagc ttggtgagca ccacaccggt gacctcggtg
     1261 gtgttgccga actcgcgcgc ctgggccaag ccgttctggc cggtggtggc gtcgatgacc
     1321 agcagcgtct cggtgacgcg ccccttgccc ttgctggcga cgcggcgcac cttgcgcagc
     1381 tcttccatgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnngca cgatgtcggc gccggagcgt tcggcccagg tgccgagctg ttcggccgcg
     1561 gcggcgcgga acgtgtcgcc cgcggccatc aagacgctcc ggccggactc gatctgcgcc
     1621 gcgccgacct tgccgatcgt agtggtcttg ccgacaccgt tgacgccgac gaacagccac
     1681 acgttgggcg agtcgggctc tccgggttcg aagtgcagcg tgcgatccgc tccggcgagg
     1741 cgggcgacca tctcggcctg ca
//
LOCUS       DAORNF010000733         1761 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_48633_length_1761_cov_1.305979, whole genome shotgun sequence.
ACCESSION   DAORNF010000733 DAORNF010000000
VERSION     DAORNF010000733.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1761
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48633_length_1761_cov_1.305979"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>208)
                     /locus_tag="PLV68_20635"
     CDS             complement(<1..>208)
                     /locus_tag="PLV68_20635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cyclohexanone monooxygenase"
                     /protein_id="HRE03714.1"
                     /translation="NTAANDLIAEFVRNKIRTVVDDPEVAETLCPTSFPIGAKRLCVD
                     TNYYATFNRPNVSLIDIRKRPIRAI"
     assembly_gap    209..452
                     /estimated_length=244
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<453..1181)
                     /locus_tag="PLV68_20640"
     CDS             complement(<453..1181)
                     /locus_tag="PLV68_20640"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRE03715.1"
                     /translation="MTVTTTADGSATAGSNTLTASDAHAGVGQPGAVDVDVVVVGAGF
                     AGMYLLVRLRELGLSTVVLETAGDVGGTWYWNRYPGARCDIQSLDYSYSWDPELEETW
                     EWSEKYATQPEILRYAQHVASKHDLRRDMRFGTRVVAAVWDEVGSRWSIETAPAEANA
                     GSDDGAGSGTITCRYYVMATGCLSVPKEIDIAGVERFAGAVYYTGRWPHAGVDFTGKR
                     VAVIGTGSSAIQSIPLIAAQAGHVT"
     gene            complement(1305..>1761)
                     /locus_tag="PLV68_20645"
     CDS             complement(1305..>1761)
                     /locus_tag="PLV68_20645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRE03716.1"
                     /translation="NITPGYWNKPEATAAAFTDGWLHTGDAARVDDEGFIYIVDRWKD
                     MYISGGENVYPAEVENVLMAHPGIADVAVIGVPDEKWGETAKAIVVKTADSDVDEQSI
                     IDFARERLAKFKCPTSVDWIDALPRNPSGKVLKKDLRAPYWVGRERNVN"
BASE COUNT          219 a          506 c          516 g          276 t
ORIGIN      
        1 gatcgcgcgg atcggccgct tgcggatgtc gatcaacgac acgttgggac ggttgaacgt
       61 ggcgtagtag ttggtgtcga cgcacaaccg cttggctccg atggggaacg aggtcgggca
      121 cagtgtctcg gccacctcag ggtcgtcgac caccgtccgg atcttgttgc ggacgaactc
      181 ggcgatcaga tcgttggccg ccgtgttgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncgtgacgt ggccggcctg ggcggcgatc
      481 agcgggatcg actggatcgc cgacgagccg gtgccgatca ccgccacccg cttgccggtg
      541 aagtcgacgc cggcgtgggg ccaacggccg gtgtagtaga cggcgccggc gaagcgctcg
      601 actccggcga tgtcgatttc cttgggtacc gacaggcacc cagtggccat cacgtagtag
      661 cggcaggtga tcgtgcccga accagcgcca tcgtcgctac ctgcgttggc ttcggctggc
      721 gcggtctcga tcgaccaccg cgagccgacc tcgtcccaca cggcggcgac gacccgggtg
      781 ccgaagcgca tgtcgcggcg caggtcgtgc ttgctggcga catgttgggc gtagcgcagg
      841 atctcgggct gagtcgcgta cttctcgctc cactcccacg tctcttccaa ctcggggtcc
      901 cacgagtagg agtagtcgag gctctggatg tcgcagcgcg cccccgggta gcggttccag
      961 taccacgtgc cgccgacgtc gccggccgtc tccagcacca cggtggacag gcccagctca
     1021 cgcaggcgca ccagcagata catgcccgcg aagccggcgc cgaccacgac cacatcgacg
     1081 tcgacagcgc caggctggcc gacacccgcg tgggcgtcgc tggcggtgag tgtgttgctg
     1141 ccagcggtgg cgctgccatc cgccgtggta gtgacagtca tgtcgttttc ccccttcgat
     1201 cacgagcgat ggccggcagg ccgtgcacgg cgccactctc gcgcaccagc cgtttccgct
     1261 gcgatccgag gggcgggtcg cttgcgatcg tggtgtgacg tgggtcagtt gacgttgcgc
     1321 tcgcgtccaa cccagtacgg cgcacggagg tccttcttca gcaccttgcc gctggggttg
     1381 cgcggcaggg cgtcgatcca gtcgacgctg gtcgggcact tgaacttggc caaccgctcg
     1441 cgggcgaagt cgatgatcga ctgctcgtcg acgtcgctgt ccgccgtctt gacgacgatc
     1501 gccttggcgg tctcacccca cttctcgtcg ggcacgccga tcactgcgac gtcggcgatg
     1561 ccggggtggg ccatcagcac gttctcgacc tcggccgggt acacgttctc gccgccggag
     1621 atgtacatgt ccttccagcg gtcgacgatg tagatgaagc cctcgtcgtc gacccgggcc
     1681 gcgtcgccgg tatgcagcca tccgtcggtg aacgccgccg ccgtcgcctc cggcttgttc
     1741 cagtagccgg gcgtgatgtt g
//
LOCUS       DAORNF010000734         1754 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_48871_length_1754_cov_1.764567, whole genome shotgun sequence.
ACCESSION   DAORNF010000734 DAORNF010000000
VERSION     DAORNF010000734.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1754
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48871_length_1754_cov_1.764567"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..928
                     /locus_tag="PLV68_20650"
     CDS             <1..928
                     /locus_tag="PLV68_20650"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRE03717.1"
                     /translation="AGSVQHLPTPSDSAEVPAVFAAALAPLHGQPALVAITGASNVTG
                     EVLPIATIVEAAHRYGARVVVDAAQLAPHRAIDIATLDVDYVAFSGHKLYAPFGAGAL
                     VGRRDWLDDAPPYLSGGGATYHVTVDDVGWYPAPARHEAGSPNVIGAVAIAAACDTLT
                     ATGFDAIARHDEVLTMGLLDGLDRIDGVEVVSLFGADHDRVGIVTVQTQRPAAIVAAA
                     LSAEHGIATRDGAFCAHPLLHRLLPPLPTGHVPNALRVSVGVGSRADDVTVLLDALGS
                     IAAEGPQWKYHAPAGRYIPNPDPRPRPSGIAG"
     assembly_gap    954..972
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<973..1330)
                     /locus_tag="PLV68_20655"
     CDS             complement(<973..1330)
                     /locus_tag="PLV68_20655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4280 domain-containing protein"
                     /protein_id="HRE03718.1"
                     /translation="MTPAVMGSMMMCTFGVAPSTLVVLPTNQVMGSKLPMANINDAKP
                     IVNIPPFGMCTSLSNPTVAAATASAAGVLTPMPCIPVTTVWMPGKPTTMVAKMPAVDI
                     TCKAMCNWGGSISFVNS"
     assembly_gap    1356..1365
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1399..>1754
                     /locus_tag="PLV68_20660"
     CDS             1399..>1754
                     /locus_tag="PLV68_20660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03719.1"
                     /translation="MPAPPDTPPSIPAWRMLGTVSIGVLAGEAPHAEHEGQLDAVRAQ
                     FLTELTQPSPFATVVANAALDSAAAMLLAIAVSCELDIALARAVSRVNDDLARPRLTL
                     QIADRLMRDVGSSVADV"
BASE COUNT          246 a          633 c          553 g          293 t
ORIGIN      
        1 cgccgggtcg gtgcagcacc tgcccacacc gtcggactcc gccgaggttc ccgccgtctt
       61 tgccgccgcg ctcgccccgc tccacgggca accggcgttg gtcgcgatca caggtgccag
      121 caacgtgacg ggcgaagtcc ttccgattgc aacgattgtc gaggctgccc accgctacgg
      181 agcgcgcgta gtggtcgacg ccgcacaact cgcaccgcat cgcgcgatcg acatcgccac
      241 gctcgatgtc gactacgtcg ccttctcggg acacaagctg tacgccccgt tcggggccgg
      301 ggcgctcgtc ggccgtcgcg actggctcga cgacgcgccc ccctatctgt cgggcggcgg
      361 agccacttac cacgtcaccg tcgacgacgt cggctggtac ccggcgcccg cccggcacga
      421 ggcaggctcg ccgaacgtga tcggtgccgt ggccatcgct gccgcgtgcg acacgctcac
      481 cgcgacgggg ttcgatgcga tcgcccgcca cgacgaggtc ttgacgatgg ggttgctcga
      541 cgggctcgac cggatcgacg gtgtcgaggt cgtctcgttg ttcggcgctg accacgaccg
      601 tgtcgggatc gtcaccgtgc agacccagcg ccctgccgcg atcgtcgcgg ccgctctcag
      661 cgccgagcac ggcatcgcca cgcgagacgg tgcgttctgc gcccacccac ttctgcaccg
      721 tctgctgccg ccgctgccca ccggtcacgt tcccaatgcg ctgcgcgtca gcgtcggtgt
      781 cggctcgcgg gccgacgacg tgacggtcct gctcgacgcg ttgggctcga tcgcagccga
      841 aggcccgcag tggaagtacc acgcccccgc cggtcgctac atacccaacc cggacccgcg
      901 cccgcgtccg tccggcattg ctgggtgagg tcgcctattt gatgttgatc gtgnnnnnnn
      961 nnnnnnnnnn nngggagttg acgaagctga tcgatccacc ccagttgcac atcgccttgc
     1021 aggtgatgtc gaccgcgggc atcttggcca ccatcgtcgt cggcttgccg ggcatccaca
     1081 cggtggtcac cggaatgcac ggcatgggcg tcagcacccc ggcggccgag gccgtggccg
     1141 ccgccaccgt cgggttgctg aggctggtgc acatcccgaa cggcgggatg ttgacgatcg
     1201 gcttggcgtc gttgatgttg gccatcggca acttcgaccc catcacctgg ttggtcggca
     1261 acacgaccaa cgtcgagggg gccacaccga aggtgcacat catcatcgaa cccatgacgg
     1321 ctggagtcac aggcccgacc ctactcctct cgccgnnnnn nnnnncccca tgcccgtgac
     1381 ctgcttgact ggtcggcgat gcccgcgcca cctgacacgc ccccttcaat tccggcgtgg
     1441 cggatgctcg gcacggtgtc gatcggcgtg ctcgccggcg aagccccaca cgctgagcac
     1501 gagggccagc tcgacgcggt gcgggcacag ttcttgaccg agctcaccca gccatcgccc
     1561 tttgccaccg tcgtcgccaa cgccgcgctc gattcggccg cggcgatgct gctggccatc
     1621 gccgtgagtt gcgaactcga cattgccttg gcgcgcgccg tgtctcgcgt caacgacgac
     1681 ctggctcggc cacgtctcac gctgcagatc gcggaccggt tgatgcgcga cgtcggttcg
     1741 tccgtcgcgg acgt
//
LOCUS       DAORNF010000735         1753 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_48904_length_1753_cov_1.842756, whole genome shotgun sequence.
ACCESSION   DAORNF010000735 DAORNF010000000
VERSION     DAORNF010000735.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48904_length_1753_cov_1.842756"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /locus_tag="PLV68_20665"
     CDS             complement(<1..310)
                     /locus_tag="PLV68_20665"
                     /inference="COORDINATES: protein motif:HMM:NF019342.2"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HRE03720.1"
                     /translation="MRAPLIVIVGRLSPQAKNVRGEAFAHGRHYAHAVERAGGVPLML
                     PPIPALTDDRLHDLLQRVDGALFHGGGDIDPSHYGAALGETTPGIVAEHDTVELALMR
                     A"
     gene            complement(356..727)
                     /locus_tag="PLV68_20670"
     CDS             complement(356..727)
                     /locus_tag="PLV68_20670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRE03721.1"
                     /translation="MMRLGTVVLGAEDAERAAAFWAAVFGYEIVRFEGSENGFTILVP
                     PDRVGTRIAVQRSETPVQERPRVHVDLVVDSADEQRREIDRLIGLGASRVAWQYDADP
                     DYVVLADTEGNRFCIVDASHG"
     gene            complement(753..1457)
                     /locus_tag="PLV68_20675"
     CDS             complement(753..1457)
                     /locus_tag="PLV68_20675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03722.1"
                     /translation="MAESLGYTVTPFDFRVPGVTSISADTHKYGYALKGSSVLLFRPQ
                     ELRRAQYFMQQGWPGGAYASPSMGGSRSGGLIAATWAAMLNLGKQGYRDIAAAIFRTA
                     AEVKDAVRAHPELTTIGDSLFNIAFTASTPASDGGIDIFHVNDALATRGWRLNGLQAP
                     PALHFCITRPNTQPGVTEAFAADLTAAVEYARNPPSPKPKSGASYGSGGRAVDHDRLR
                     AGTFAYLDAVHTLGPH"
     gene            complement(1430..>1753)
                     /locus_tag="PLV68_20680"
     CDS             complement(1430..>1753)
                     /locus_tag="PLV68_20680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HRE03723.1"
                     /translation="IEQPEVILPVTAHPALDKAMHYFGIRGVKAPVTAGFDSGFVADV
                     DAARSLISPNTVAIVGSAGTYPHGLIDPITELGQVALDHGIGLHVDGCLGGFVLAWPS
                     RWVTP"
BASE COUNT          275 a          587 c          623 g          268 t
ORIGIN      
        1 cggcccgcat cagcgcgagc tcgacggtgt cgtgttcggc cacgatgcca ggggtggtct
       61 cgccgagagc cgccccgtag tgactcgggt cgatgtcgcc accgccgtgg aacaacgcgc
      121 cgtcgacgcg ctggagcagg tcgtgcaacc ggtcgtctgt caacgccggg atcggcggca
      181 gcatcagcgg cacgccaccg gcccgttcga cggcatgggc gtagtgccgc ccgtgagcga
      241 acgcctcgcc gcgtacgttc ttcgcctggg gactcaaccg accgacgatg acgatgagcg
      301 gtgcgcgcac gggacgcatc gtatgtgagt cgtccccggg cgactcagcc gtgactcacc
      361 cgtggctggc gtcgacgatg cagaaccggt tgccctccgt gtcggccagc acgacgtagt
      421 cgggatcggc gtcgtactgc cacgcgaccc gcgaggcgcc gaggccgatc agccggtcga
      481 tttcccgacg ctgttcgtcg gcgctgtcga ccacgaggtc gacgtgaaca cggggccgct
      541 cctgcaccgg cgtctcggac cgctgcaccg cgatgcgcgt gcccacccga tcgggcggga
      601 ccaggatcgt gaacccgttc tccgaaccct cgaatcgcac gatctcgtag ccgaacaccg
      661 cagcccagaa cgcggctgcc cgctcggcgt cttcggcccc caacacgacc gtccccaacc
      721 gcatcatgtc cccatcctgc catcagggat ggtcagtggg ggccgagggt gtggacggcg
      781 tcgaggtagg cgaaggtgcc ggcgcggagg cggtcgtggt cgacagcgcg cccgccagag
      841 ccatagctgg cgccgctctt gggcttgggg gacggcgggt tgcgggcgta ttcgaccgcg
      901 gcggtgaggt cggccgcgaa cgcctccgtg acgccgggtt gggtgttggg gcgggtgatg
      961 cagaagtgca gcgcgggcgg cgcctgcaat ccgttgagcc gccaaccgcg ggtagcgagc
     1021 gcgtcattga cgtggaagat gtcgatgccg ccgtcgctcg ccggggtgct ggccgtgaac
     1081 gcgatgttga acagcgagtc accgatcgtg gtgagttcag ggtgtgcgcg caccgcatcc
     1141 ttcacctcgg ccgcggtgcg gaagatcgcc gccgcgatgt cgcggtagcc ctgcttgcca
     1201 aggttcagca tcgccgccca tgtcgccgcg atcagcccac ccgagcggct gccgcccatt
     1261 gacggcgagg cgtacgctcc acccggccac ccctgctgca tgaagtactg cgcgcgacga
     1321 agttcttgcg gacggaacaa cagcaccgag ctgcccttga gcgcgtagcc gtacttgtgg
     1381 gtgtcggccg agatcgacgt gacacccgga acgcggaagt cgaacggcgt cacggtgtaa
     1441 cccagcgact cggccacgcg agcacgaacc cgccgaggca gccgtcgacg tgcagtccga
     1501 tgccgtggtc gagcgcgacc tggccgagtt cggtgattgg gtcgatcaac ccgtgtgggt
     1561 aggtgcccgc cgaaccgacg atcgcgaccg tgttgggcga gatcaacgaa cgcgccgcgt
     1621 cgacgtcggc gacgaagccc gagtcgaagc ccgcggtgac gggtgccttc acgccgcgga
     1681 taccgaagta gtgcatcgcc ttgtcgagcg ccgggtgcgc ggtgacgggc aggatcacct
     1741 cgggctgctc gat
//
LOCUS       DAORNF010000736         1748 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_49071_length_1748_cov_1.711754, whole genome shotgun sequence.
ACCESSION   DAORNF010000736 DAORNF010000000
VERSION     DAORNF010000736.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1748
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49071_length_1748_cov_1.711754"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1194
                     /locus_tag="PLV68_20685"
     CDS             <1..1194
                     /locus_tag="PLV68_20685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03724.1"
                     /translation="VFLTRDQRDRGSTATTTTATTLDTAPVISQATITSTTAAPTTTT
                     APPQTTTTLAETIVEPTTTVTEPPPPPPPPIPPIPEGAADLGNGVALPIPAGFVRASG
                     ALTSMVTLTNNQSVISAGSSIRQPGIDPMAITQEYVNAFDRTFDTIAYSPTARNATLP
                     GEPPTDVYTTFFVTYDVATATSQSGTIEVYIRADGLVLVLSVYGPTGASGVLPSDAMQ
                     VVNDSFQVTPLVGEPAPLAEFAPFRVTSAHPAVVVDGLTTFSPTPGFTIVSSGNGRGV
                     VTRDGVLDMQIDKYSGQVDTATVTASAQAVLAQRYTALAYSQLWRGDPDPWGVVHGAF
                     TFIGTDADGRPGTGMIDLYLDPATANALVSFRTWANPNSENNPFPAETNYMLQSLLNS
                     FTNIP"
     assembly_gap    1389..1474
                     /estimated_length=86
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1475..>1748)
                     /locus_tag="PLV68_20690"
     CDS             complement(<1475..>1748)
                     /locus_tag="PLV68_20690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03725.1"
                     /translation="EVRCRERHRATREVAPHVPDAWNPQRAAEIDGRQPEGEQLGYQG
                     PDQGYVLTLADVVRDQIITGPAESVDDAVRGSINIALKRASLYGRAP"
BASE COUNT          274 a          636 c          508 g          244 t
ORIGIN      
        1 gtgttcctga cccgcgatca acgcgaccga gggtcgaccg cgacaacgac cacggcgacc
       61 acactcgaca cggccccggt catatcacaa gccaccatca cctccaccac cgcggcgccg
      121 acgaccacca ccgcgccgcc ccagaccacg accacgctgg ccgaaaccat cgtcgagccg
      181 acgaccacgg tcaccgagcc accaccaccg ccaccgccgc cgattccgcc catccccgaa
      241 ggcgcggccg atctcggcaa cggtgtcgcg ctgccgatcc cggccgggtt cgtgcgcgcg
      301 tcgggcgcgc tgacctcgat ggtgaccttg accaacaacc agtcggtgat cagcgccggg
      361 tccagcattc gccagccagg catcgacccg atggcgatca cccaggagta cgtcaacgcc
      421 ttcgaccgca ccttcgacac catcgcctac agccccacgg cccgcaacgc caccctgccc
      481 ggcgagccgc cgaccgacgt ctacaccacg ttcttcgtca cctacgacgt cgccactgcc
      541 acatcgcaaa gtggcaccat cgaggtctac atccgtgccg acggtctggt gctggtgctc
      601 agcgtctacg gccccaccgg tgcgtcgggt gtgctgccgt cggacgcgat gcaggtcgtc
      661 aacgacagct tccaggtcac cccactcgtc ggcgaacccg cgccgctggc cgagttcgca
      721 ccgttccggg tcacctcggc ccaccccgcc gtagtggtcg acgggctgac cacgttcagc
      781 cccaccccgg ggttcaccat cgtcagctcg ggcaacgggc ggggcgttgt cacccgcgat
      841 ggcgtgctcg acatgcagat cgacaagtac tccggccagg tcgacaccgc gaccgtcacc
      901 gcctcggccc aagcggtgct cgcccagcgc tacaccgctc tggcctactc acagctctgg
      961 cgcggcgacc ccgacccgtg gggagtggtg cacggtgcgt tcaccttcat cggcaccgac
     1021 gccgatggac gtcccggcac cggcatgatc gacttgtacc tcgacccggc caccgccaac
     1081 gctctggtct cgttccgcac ctgggccaat cccaacagcg agaacaaccc cttccccgcc
     1141 gagaccaact acatgctcca gtccctgctc aacagcttca ccaacatccc gtaaggctgc
     1201 tgacgcgggc aggtcacgcc acggcggtca ggcgccggtc agggaccgcc agttgccggc
     1261 gttgtaggag gcggtggcgg cggcgggagt gaggcgaagg gtgtcttcgg ggacgagatc
     1321 ggcgatgagg cgaccttcgg cgtagtggtg gttgacgttg gccacacctt cgaacagatc
     1381 gcgccgacnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnggcgcc cgtccgtaga gcgaggcgcg
     1501 cttgagggcg atgttgatcg agccgcgcac ggcgtcgtcg accgactcgg ccgggccggt
     1561 gatgatctgg tcgcgcacga cgtcggccag ggtcagcacg tatccctgat ctggcccctg
     1621 gtagccgagt tgctcaccct cgggctggcg gccgtcgatc tcggccgccc gctgcggatt
     1681 ccacgcatcg ggcacgtgcg gggcgacctc gcgggtggcc cggtgccgtt cgcggcaacg
     1741 cacttcgc
//
LOCUS       DAORNF010000737         1744 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_49210_length_1744_cov_1.490231, whole genome shotgun sequence.
ACCESSION   DAORNF010000737 DAORNF010000000
VERSION     DAORNF010000737.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49210_length_1744_cov_1.490231"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="PLV68_20695"
     CDS             <1..205
                     /locus_tag="PLV68_20695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03726.1"
                     /translation="TPLSHGIVAAAGTFVAVQAVLVVINLVRGADVHWMNIFFNLTVT
                     VVAGLIGGLLGMNMLRRGVEPRR"
     assembly_gap    261..326
                     /estimated_length=66
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <327..1725
                     /locus_tag="PLV68_20700"
     CDS             <327..1725
                     /locus_tag="PLV68_20700"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HRE03727.1"
                     /translation="FPPDSPSPGLVQFDAMALARVVLDVAHATIAAATAAGEQIAALG
                     ITAQRATTIVWDRTTGVPIGPAIGWQDLRTVIECIMAKAEHGLALAPNQSATKIGWLL
                     ANHAPHTTNPLRDGDAGQRGDLAFGTVDSWIAWVLSGGTAHITDHTNAAVTGLYDLAS
                     GGWSEPACAALGVPMEMLPTIVDSSGELAIASVLPGAPPICGLAGDQQASLVGQGCIH
                     PGRAKITFGTGGMLDVCTGLDTPTTANRSANGTFPIVAWSQAGTRRFGTEAIMLSAGT
                     NVEWLCDDMGLIETPADSDAVARQCDSTDGVVYVPALLGLGTPQWDYGARGALFGVTR
                     GTTRAHVVRAVLEGVAQRGADLVESAEADFGRPIPALRIDGGMSQNPTFVQALADATQ
                     RPVQVSAVTEATTIGAGLLAGVAAGVWPSLDAVADSTATSSVVDPQASFDRDRWREAV
                     SRTTHWIPDLSSLDF"
BASE COUNT          246 a          613 c          559 g          260 t
ORIGIN      
        1 gacgccgctc tcgcatggca tcgtcgccgc ggcgggcacc ttcgtcgcgg tgcaggcggt
       61 gctggtggtg atcaacctgg tgcgcggcgc cgacgtgcac tggatgaaca tcttcttcaa
      121 cctcacggtc accgtcgtgg ccggtctgat cggaggtctg ctcggtatga acatgttgcg
      181 ccgtggtgtg gagccccggc gatgagcgcg tccacacggc cttcgacact cgtcgtcgac
      241 gtcggcacca gcggcctgcg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnncttc ccgcccgatt cgccatcgcc ggggttggtc
      361 cagttcgacg cgatggccct cgcccgcgtc gtgctcgacg tcgcccacgc caccatcgcg
      421 gccgcgaccg cggcaggcga acagatcgcc gccctcggca tcaccgcgca gcgggccacg
      481 acgatcgtgt gggaccgcac caccggggtc cccatcggcc cggcgatcgg ttggcaggac
      541 ctgcgcacgg tgatcgagtg catcatggcc aaggccgagc acggtctggc cctggccccc
      601 aaccagtcgg ccaccaagat cggctggcta ctcgccaacc acgcgccgca caccaccaat
      661 ccgctgcgtg acggcgatgc cggccaacgc ggcgatctgg ccttcggtac ggtcgattcg
      721 tggatcgcgt gggtgctgtc cgggggcacc gcccacatca ccgatcacac caacgccgcg
      781 gtcacgggcc tttacgacct ggccagcggc gggtggtcgg agccggcctg cgccgcgttg
      841 ggcgtaccga tggagatgct acccaccatc gtcgattcgt ctggcgagtt ggcgatcgcc
      901 tcggtgcttc ccggcgcccc gccgatctgt ggcctcgccg gcgaccaaca ggcgtcgctg
      961 gtggggcaag ggtgcatcca ccccggccgg gccaagatca cgttcgggac cgggggcatg
     1021 ctcgacgtct gcacgggtct cgacacaccg accactgcca accgcagcgc caacggcacc
     1081 ttcccgatcg tggcctggtc acaagcgggc acccgccgct tcggcaccga ggcgatcatg
     1141 ctcagcgccg gcaccaacgt cgagtggctg tgcgacgaca tgggcctgat cgagacgccg
     1201 gccgacagcg atgccgtcgc ccgccagtgc gactcgaccg acggggtcgt ctacgtgcca
     1261 gccttgttgg ggctcggcac cccgcagtgg gactacggcg cccgcggcgc gctgttcggc
     1321 gtcacccgtg gcacgacgcg cgctcacgtc gtgcgagccg tgttggaggg cgtcgcccaa
     1381 cgcggtgccg acctggtcga atcggccgag gcagacttcg gccgtccgat cccggccctg
     1441 cgcatcgacg gcggaatgag ccagaacccg accttcgtgc aggcgctcgc cgacgccacc
     1501 caacgcccgg tgcaggtctc ggccgtgacc gaagccacca cgatcggtgc cgggttgctg
     1561 gccggggtgg ccgctggcgt gtggcccagc ctcgatgccg tggcagactc gacggcgaca
     1621 tcgtcagtcg tcgacccaca agccagcttc gaccgcgatc gctggcgcga agcagtctcc
     1681 cgaacgacgc actggatacc cgacctgtcg tcgctcgact tctaaagttg gtgcgcagcc
     1741 tctc
//
LOCUS       DAORNF010000738         1741 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_49317_length_1741_cov_1.792408, whole genome shotgun sequence.
ACCESSION   DAORNF010000738 DAORNF010000000
VERSION     DAORNF010000738.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1741
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49317_length_1741_cov_1.792408"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1741)
                     /locus_tag="PLV68_20705"
     CDS             complement(<1..>1741)
                     /locus_tag="PLV68_20705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03728.1"
                     /translation="VRDESLVFTDRIENVPEEYRQVGTSILMENARYIDYHAIGWYDS
                     PQLFERRDEAGRLLTLSRQNWPVDLLTKAVQPRSERVHFHLMVPRKEISARGEGKPAP
                     TVIIGHGYTSNRFDLMIIGSYLVRHGVAVLAIDSVSHGIVLSPDQASQAQGIIELFGL
                     KSFLTAITDRDRALDLDGDGVDDSGGDFWTAYLFHTRDNVRQTALDYMQLTRILRTFD
                     GSKQWAFAPFSNDPNLPKIAGDFDGDGEVDLGGDAPITMLGASLGGIMSAVVGGVEPE
                     IKTTVPIAAGGGLTDVSPRSIQGGVKEAIVLRLMGPIYLGTGNSTAFAIDTVVPIVND
                     DETLRLGSLKGVQAGDFLVVENLTNKERGCGWVRDEAGTLRARAHLPSDTGDHVRVVV
                     YEGDAMILGDKECGVKADATVRTSLEHFGQDVTFHGRKHKQGEPLISLAEGAGLRRAS
                     PDLRRMFSLGQMIIDRTDPAVMSQYIAKKSFTYPGTGQSTGHGRTLVVTTVGDMNVPA
                     STGITIGRAAGLIDYLNPMPGHDVPMNQVLIDNYTAEATEVTKRFTNPAGQGVHMDVE
                     NFSNGGDFWLTDIP"
BASE COUNT          280 a          527 c          615 g          319 t
ORIGIN      
        1 gggatgtcgg tgagccagaa gtcaccgccg ttgctgaagt tttcgacgtc catgtggacg
       61 ccttggccgg ccgggttggt gaagcgtttc gtgacctcgg tcgcctcggc cgtgtagttg
      121 tcgatgagga cctggttcat cggcacgtcg tggccgggca tcggattgag gtagtcgatg
      181 aggccggcgg cgcgaccgat ggtgatgccg gtcgacgcag gcacgttcat gtcgccaacc
      241 gtcgtcacga cgagtgtccg accatggccc gtcgactggc cggtgcccgg gtaggtgaac
      301 gacttcttgg cgatgtactg cgacatgacg gccgggtcgg tgcggtcgat gatcatctga
      361 ccgagcgaga acatgcgacg caggtcgggg ctcgcgcggc gaagacccgc gccctcggcg
      421 agcgagatga gcggctcgcc ctgtttgtgt ttgcggccat ggaaggtcac gtcctggccg
      481 aagtgctcga ggctcgtacg gacggtcgcg tcggccttca cgccgcactc tttgtcgccc
      541 agaatcatcg cgtcaccctc gtagacgacc acgcgcacgt ggtcgccggt gtcgctcggc
      601 aaatgcgcgc gggccctgag tgtgcccgcc tcgtcgcgaa cccagccgca gccgcgctct
      661 ttgttggtga ggttttcgac cacgaggaag tcacccgcct gcacgccttt cagcgacccg
      721 agtcgcagcg tctcgtcgtc gttgacgatg gggacgaccg tatcgatggc gaacgcggtg
      781 ctgttgccgg tgccgaggta gatcggcccc atgagccgca atacgatcgc ttccttgacg
      841 ccgccctgga tggagcgcgg gctgacgtcg gtcaggccac cacccgccgc gatgggaacc
      901 gtcgtcttga tctcgggctc gacaccgccg accaccgccg acatgatgcc gccaagcgac
      961 gcgccgagca tcgtgatggg ggcatcgccg cccaggtcga cctctccgtc gccgtcgaag
     1021 tcgccagcga tttttggcag attcgggtca ttggaaaatg gtgcgaacgc ccactgcttg
     1081 ctgccatcga acgtccgcag gatgcgtgtg agctgcatat agtcgagcgc ggtctgccgc
     1141 acgttgtcgc gcgtgtggaa gagataggcg gtccagaagt cgccgcccga gtcgtcgacg
     1201 ccgtcgccgt cgaggtcgag ggcgcggtcg cgatcggtga tggccgtgag gaaggacttg
     1261 aggccgaaga gctcgatgat gccctgggcc tgcgaggcct ggtcggggct caagacgatg
     1321 ccgtgcgaga cgctgtcgat ggccagcacc gcgacgccgt ggcgcacgag gtagctgccg
     1381 atgatcatga gatcgaaacg gttcgaggtg tagccgtgac cgatgatgac ggtcggcgcg
     1441 ggcttgcctt cgccacgggc ggagatctct ttgcgcggca ccatgaggtg gaagtggaca
     1501 cgctcggagc gtggctgaac cgccttcgtg agcaggtcca ccggccagtt ctggcgcgag
     1561 agagtcagga ggcggccggc ctcgtcacgg cgctcgaaga gctgcggcga gtcgtaccag
     1621 ccgatggcgt ggtagtcgat gtagcgcgcg ttctccatga ggatggaggt gccgacctgc
     1681 cgatactcct cggggacgtt ctcgatgcga tcggtgaaaa ccagcgactc gtccctgacg
     1741 c
//
LOCUS       DAORNF010000739         1732 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_49655_length_1732_cov_1.444246, whole genome shotgun sequence.
ACCESSION   DAORNF010000739 DAORNF010000000
VERSION     DAORNF010000739.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49655_length_1732_cov_1.444246"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..869)
                     /locus_tag="PLV68_20710"
     CDS             complement(<1..869)
                     /locus_tag="PLV68_20710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03729.1"
                     /translation="MFDWAIGGLGALFGFAGDAIGGAIGWAWDKVIQGIYTWLANGLA
                     LLVEWVWSVLDSSTTPRLTEDWFRNELSARVGLIGLAVVVAMMLASAIQAALAGRPEQ
                     IGDAVKEGVRAIVASALTVTAIDVLIGVTDEASASVWQVGRADLVAMIEGIVVVATTT
                     GPLGATFVGPLCLLFGFIGLIGLVVALLMRSALIYVAAALAPIVWSTNVLPMFRGSSR
                     KLVHLLVALVVSKLAIVVTLVVAVKLIANPSGDPTSGSVINDGAAAVGTLMSGFVCFL
                     IAAVTPFVLYKLMP"
     gene            complement(869..1537)
                     /locus_tag="PLV68_20715"
     CDS             complement(869..1537)
                     /locus_tag="PLV68_20715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03730.1"
                     /translation="MVLSLGAAIVAVVVGVRVLASTDDSPADSAPPPVADAPDATMPT
                     GPTAVDEFGVPQGWRRDPAGALSAAVSAVQLTGPIARAGFITRSDMIASLASDRYGPT
                     LASESANQLAEMTAVLGEASVAPDDLVWSELPLTAQVVHADDRVAQVELWSVLVVGVR
                     DVGAPRQAWRTVTVDLVWEADDWKVGGWSSQPGPTPLLDSMVAVASTSEIDEVVSWAP
                     VGGG"
     gene            complement(1552..>1732)
                     /locus_tag="PLV68_20720"
     CDS             complement(1552..>1732)
                     /locus_tag="PLV68_20720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03731.1"
                     /translation="AIAAAVAGLLIGGVATGVGHFGSSYSASSAGRKWVLGGIGAAML
                     AGLAHTVAITVYNAT"
BASE COUNT          325 a          671 c          529 g          207 t
ORIGIN      
        1 ggcatcagct tgtacaacac gaacggcgtg accgccgcga tcagaaagca cacgaacccc
       61 gacatcaacg tgccgaccgc ggccgcgccg tcgttgatca ccgagccgct ggtcgggtca
      121 ccggacgggt tggcgatcaa cttcaccgcc accaccaggg tcacgacgat cgccagcttc
      181 gacacgacca acgccaccaa gaggtgcacg agcttgcggg acgagccccg gaacatcggc
      241 agcacgttcg tggaccacac gatcggcgcc agcgcggccg ccacgtagat gagcgccgag
      301 cgcatcagca gcgcgaccac caacccgatc agaccgatga acccgaacaa cagacacagc
      361 gggccaacga acgtggcgcc gagcggcccg gtagttgtgg cgacgaccac gatcccctcg
      421 atcatcgcca ccaggtcggc tcggccgacc tgccacaccg acgccgacgc ctcgtcggtc
      481 accccgatca gcacgtcgat cgccgtgacc gtcaacgccg acgcgacgat cgcccggaca
      541 ccctccttga ccgcgtcacc gatctgttcg ggccggcccg cgagcgcggc ctgaatcgcg
      601 gaggccagca tcatcgccac cacgaccgcc agaccgatca gcccgacccg cgccgatagc
      661 tcgttgcgga accagtcctc cgtcaaccgc ggggtggtcg acgaatcgag caccgaccag
      721 acccactcga ccagcagtgc caggccgttg gcgagccagg tgtagatgcc ctggatgacc
      781 ttgtcccacg cccaaccgat cgcgccgccg atcgcgtcgc cggcgaagcc gaacagcgcg
      841 ccgagcccac cgattgccca gtcgaacatc acccacctcc caccggcgcc caggacacga
      901 cctcgtcgat ctcggacgtg ctcgccaccg cgaccatcga gtccagcagc ggtgtcggac
      961 ccggctggct ggaccagccg ccgaccttcc agtcgtcggc ctcccagacc agatccaccg
     1021 tcacggtcct ccacgcctgc cgcggcgcac ccacatcgcg gaccccgaca acgagcaccg
     1081 accacagctc cacctgcgcg acgcggtcgt ctgcatgaac gacctgcgcc gtgagtggga
     1141 gttcactcca caccagatca tccggtgcga ccgacgcctc gccgagcaca gcggtcatct
     1201 cggcgagctg attcgctgac tcgctcgcca gggtcggccc gtagcggtcg gacgcgagcg
     1261 acgcgatcat gtccgagcgg gtgatgaacc cagcccgcgc gatcggcccg gtcaactgca
     1321 ctgccgacac cgccgcagac aacgcacccg ccgggtcacg ccgccaaccc tggggcacgc
     1381 cgaactcgtc gaccgcggtc ggtccggtgg gcatggtcgc gtccggtgcg tcggcgacgg
     1441 gcggcggcgc gctgtcggca ggcgagtcgt cggtcgatgc cagcacgcgc acacctacga
     1501 ctacggcgac gatcgcggcg ccgagggaga ggaccatcgc gcggcgcatg gtcacgtggc
     1561 gttgtagacg gtgatcgcga cggtgtgtgc gagcccggcg agcatggctg ctccgatgcc
     1621 gcccagcacc cacttccgcc cggccgacga tgccgagtac gacgaaccga agtggccgac
     1681 ccccgtagcg acaccgccga tcaacaaccc ggcgaccgct gccgcgatcg cc
//
LOCUS       DAORNF010000740         1729 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_49752_length_1729_cov_1.489247, whole genome shotgun sequence.
ACCESSION   DAORNF010000740 DAORNF010000000
VERSION     DAORNF010000740.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49752_length_1729_cov_1.489247"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="PLV68_20725"
     CDS             <1..576
                     /locus_tag="PLV68_20725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03732.1"
                     /translation="PGAVRLVTGVRRNGTSSLDVSGVARELGGDVWVVPGPESGDQAR
                     AVWAEGGAAVADRLASDDGVWFVDAGRVDDANPSVAFVDRSVLAVMVTGPYQEDLVQL
                     PARVASLRARCGSVAVLVSGRCSFDRFEIAEFCGADVVWVVPCRDDLVDEVGRLVGGG
                     RARRSWLWRHALDVAADAYGLVASRAAGVAS"
     gene            573..>1025
                     /locus_tag="PLV68_20730"
     CDS             573..>1025
                     /locus_tag="PLV68_20730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03733.1"
                     /translation="MIVDAVRVDPALVSRLSLSVQEALVAEATERAKAGRSGLTRAGQ
                     EALAESVLRDELQRIDDDRLAAGSGMLDDSTQRSLVERVLALSVGLGPVELLLADPMV
                     EEIVATRFDLVFVYRSDGTVEEVDERLWASDADLATWLSHLARTAGRTE"
     assembly_gap    1026..1171
                     /estimated_length=146
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1172..>1415
                     /locus_tag="PLV68_20735"
     CDS             <1172..>1415
                     /locus_tag="PLV68_20735"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HRE03734.1"
                     /translation="MSPVIADLLRACMRSSEIRIVFSGPTGSGKTTLVRACLGELPRL
                     ARVVIIEDTAEIDLFDPVSHPNVESWEARLANSEGEG"
     assembly_gap    1416..1498
                     /estimated_length=83
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          165 a          384 c          658 g          293 t
ORIGIN      
        1 ccgggcgctg tgcggctggt gacgggtgtg cggcggaacg ggacgtcgtc gctcgacgtg
       61 tcgggtgtgg ctcgggagtt gggcggtgac gtgtgggtgg tgccgggccc ggagtcgggg
      121 gatcaggcgc gggcggtgtg ggcggagggc ggtgctgcgg ttgctgaccg gctggcgagt
      181 gatgatggcg tgtggttcgt cgacgccggc cgggtggacg atgcgaaccc gagcgtggcg
      241 ttcgtcgacc ggtcggtgtt ggcggtgatg gtgaccggcc cgtatcagga ggatctggtg
      301 cagttgccgg cgcgggtggc gtcgttgcgt gcccggtgcg ggtcggtggc ggtgttggtg
      361 tcgggtcgtt gttcgttcga ccgcttcgag atcgccgagt tctgcggtgc ggatgtggtg
      421 tgggtggtgc cctgccgtga cgacttggtc gacgaggtcg gccggttggt gggtggtggt
      481 cgggctcgcc ggtcgtggtt gtggcggcac gcgctcgacg ttgctgccga cgcgtacggg
      541 ctggtcgcgt cgcgggcggc gggggtggct tcgtgatcgt cgacgcggtg cgtgtcgacc
      601 cagcactggt gtcgcgtctg tcgctgtcgg tgcaggaggc gctggtcgcc gaggcgaccg
      661 agcgcgcaaa ggccggtcgg tcggggttga cgagggccgg gcaggaggcg ttggcggagt
      721 cggtgttgcg cgacgagttg cagcgcatcg acgacgaccg tctggcggcg gggtcgggga
      781 tgttggacga ctcgacgcag cggtcgttgg tggagcgcgt gttggcgttg tcggtcgggt
      841 tgggcccggt cgagttgttg ttggccgatc cgatggtgga ggagatcgtc gcgacccgct
      901 tcgacttggt gttcgtctac cgctccgacg ggacggtgga ggaggtcgac gagcggctgt
      961 gggcgtcgga cgccgatctg gcgacgtggc tgtcgcatct ggcgcgcacg gcgggccgta
     1021 cggagnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngatgtcgcc ggtcatcgcc gatctgttgc
     1201 gggcgtgcat gcggtcgtcg gagatccgga tcgtgttctc cggtccgacc ggctcgggca
     1261 agacgacgct ggtgcgggcg tgcctgggtg agctgccgcg gctggcgcgg gtggtgatca
     1321 tcgaggacac cgccgagatc gatctgttcg accctgtgtc gcacccgaac gtcgagtcgt
     1381 gggaggcacg gctggcgaac agcgagggtg agggtnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnac
     1501 agcgacgcag cggtgccgat gttgaaggcg atgacacacg gccagtcgag tttgacaacc
     1561 gtgcatgcgg cttcggcggt gggtgcgttg gacaagctgg ccctctacct gggcaccggg
     1621 gaggatcgtc tgcccccgga ggtggcgcac agccagttgc atcaggcggt cgatttcgtg
     1681 gtgcatctcg accgtggtgt cgacgggcgg cgggcgctgg tcgagatga
//
LOCUS       DAORNF010000741         1727 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_49823_length_1727_cov_1.479067, whole genome shotgun sequence.
ACCESSION   DAORNF010000741 DAORNF010000000
VERSION     DAORNF010000741.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1727
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49823_length_1727_cov_1.479067"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..778)
                     /gene="cofD"
                     /locus_tag="PLV68_20740"
     CDS             complement(<1..778)
                     /gene="cofD"
                     /locus_tag="PLV68_20740"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HRE03735.1"
                     /translation="MTITVLAGGVGAARFLRALLLVVDPAEVTAIVNTGDDTVMHGLS
                     ISPDLDTVTYTLGGAIDPERGWGLRDETWRAMEALGRFVPQRPPGSAAAPTWFNLGDQ
                     DLATHFYRTARLAEGATLTEVTGEIARAFGVTITLLPMTDDGVETRVTVHDDGDADRG
                     AGHEVSFQEYFVQRRHSVPVSAVRFAGADEATLTVASRAALVDADTIVIAPSNPLVSI
                     GPLRALSGVDDLLRARRDRVVAISPIIAGAALKGPADRMLI"
     assembly_gap    837..940
                     /estimated_length=104
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<941..1666)
                     /locus_tag="PLV68_20745"
     CDS             complement(<941..1666)
                     /locus_tag="PLV68_20745"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRE03736.1"
                     /translation="MRPAPRAQVSVLHGTNYVVPPSRLPRVVTVYDCWFLRHPELADP
                     AVHRAGLVLRRAVRTGATVHATSHATAAAVRDLLATDAVEVIPLAAPPTRPVAVAPTM
                     DDAPAIAGRLATRPYVLALGTIERRKNLPTLIAAFGRMDSAGSPLLVIAGADGDDRAA
                     VDAAIGQLDASARERVVLTGRIDDATKQWLLAHARVLAYPSLDEGFGFPLLEAMAADV
                     PIVASTAGSIPEVAADAAVLVDAA"
BASE COUNT          234 a          544 c          632 g          213 t
ORIGIN      
        1 cgatcagcat ccggtcggcc ggtcccttta gggcggcacc ggcgatgatc ggcgagatcg
       61 cgaccacccg gtcgcgtcgg gcgcgcaaca agtcgtcgac gccggacagg gcacggagtg
      121 ggccgatcga taccagtggg ttggacggcg cgatcacgat cgtgtccgcg tcgaccagcg
      181 ccgcgcgcga ggcgacggtc agcgtggcct cgtccgcccc ggcgaagcgc accgcgctga
      241 ccggcacgct gtggcgacgc tggacgaagt actcctgaaa gctgacctcg tgcccggcgc
      301 ccctgtctgc gtcgccgtcg tcgtgcaccg tgacgcgggt ctcgacgccg tcgtcggtca
      361 tcggcaacag ggtgatcgtc accccgaagg cgcgggcgat ctcgcccgtc acctcggtga
      421 gcgtcgctcc ctcggccagg cgcgcggtgc ggtagaagtg cgtggccagg tcctggtcgc
      481 ccaggttgaa ccacgtcggc gccgccgcag atccgggcgg gcgctgggga acgaagcggc
      541 cgagcgcttc catcgcccgc cacgtctcgt cgcgcagccc ccaaccgcgc tcggggtcga
      601 tcgcgccgcc gagggtgtag gtcacggtgt cgaggtcggg ggaaatcgac aggccgtgca
      661 tcacggtgtc gtcgccggtg ttgacgatgg cggtgacctc ggccgggtcg accacgagca
      721 ggagcgccct gaggaaccgg gcggccccga cgccgcctgc gagcacggtg atggtcatga
      781 tgcctcctcg gcgatccggt ggtagagcgc ggacagggcg tccgctgtgg cggtccnnnn
      841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ggccgcgtcg acaagcaccg
      961 ccgcgtcggc ggcgacctcg gggatcgaac cggcggtcga ggcgacgatg ggaacatcgg
     1021 cggccatcgc ttccagcaac ggaaagccga acccctcgtc gagcgacggg taggcgagga
     1081 cgcgggcatg ggcgagcagc cactgcttgg tggcgtcgtc gatgcggccg gtcagcacca
     1141 cccgctcgcg ggccgaggcg tcgagttgcc cgatcgcggc atcgaccgcg gctcgatcgt
     1201 cgccgtcggc accggcgatg accagcagcg gtgagccggc gctgtccatc cgtccgaacg
     1261 cggcgatcag ggtgggcagg ttcttgcgcc gctcgattgt gccgagggcg agcacgtagg
     1321 gccgtgtggc gaggcggcct gcgatcgccg gggcgtcgtc catcgtgggg gcgacggcga
     1381 cgggacgagt cgggggagcg gcaaggggga tcacctcgac cgcatcggtg gcgagcagat
     1441 cgcgcacggc agcggccgtg gcgtgcgacg tcgcatggac ggtggcaccg gtgcgcaccg
     1501 cgcggcgcag caccagcccg gctcggtgca cggcgggatc ggcgagttct ggatggcgca
     1561 ggaaccagca gtcgtagacc gtgaccacgc ggggcaggcg gctgggcggc acgacgtagt
     1621 tggtgccatg cagcaccgac acctgcgccc ggggggcggg ccgcaaccag cgatcgacgc
     1681 ggggcatgtc ggctctggcc cacagccggt gggacaccgc cgctggc
//
LOCUS       DAORNF010000742         1726 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_49849_length_1726_cov_2.278276, whole genome shotgun sequence.
ACCESSION   DAORNF010000742 DAORNF010000000
VERSION     DAORNF010000742.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49849_length_1726_cov_2.278276"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            64..>1726
                     /locus_tag="PLV68_20750"
     CDS             64..>1726
                     /locus_tag="PLV68_20750"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRE03737.1"
                     /translation="MAWRTGAAAGLAAALGTAGLALATPSIAVAAPAAALVSPGGVPW
                     TWGANSFGQLGNGTTTARPTPGPVSGLTDVVDMHGGREHVIALRSDRTVWVWGSNGEG
                     QLGLGTTTNRPTPTQVPGLSNVQAVETGHNLSLALMGDGTVRTWGLNADGQLGDGTTT
                     LRRSPVTVSGLTNAVSIAAGRDMSYAIRADGTVVGWGRNDEGQLGDGTTVRRTTPVRV
                     GTLTNVIDIAGGRDHGLALRSDGTVWAWGSNDYGQLGDGTTTDRTTPVQVMSGVTQVI
                     AGAQHSYALRSDGTVAAWGRNYRANLGDGTTTTRTRPVTVRNLSSVVSIGSGRDTGMA
                     VLADGRVMAWGNNTTGQVGDGTTTNRTTPVVVPGVSGAVLAAGGGAEYSVVLVSSGPP
                     PPQDPVAAFSATCTVTSCDFDASGSHDPDGNLVSYDWQFGDTTTGTETVATTHHDYAT
                     TGTYSVTLTVTDNSGATASVTHDVVAEDTPPPPVGPQWRAGAAVDANSNRPTITLPAA
                     VQSGDRLLLVVSANRAATLTTPAGWTLLSTASDGTEVRSWVFTRVA"
BASE COUNT          253 a          662 c          590 g          221 t
ORIGIN      
        1 ttggatggtg cttcctcggg cacatgttcc taccgtcgag accagcggac ggaggatgtc
       61 gtggtggcgt ggcggactgg tgctgcggcg gggctcgcag ccgccctcgg gacggccggg
      121 ctcgccctgg ccaccccttc gatcgcagtg gccgctccgg ccgccgcgct ggtcagcccg
      181 ggcggagttc cctggacctg gggcgcgaac tccttcggtc agctcggcaa cggcaccacc
      241 acggcgcggc ccacccccgg tccagtgtcc gggctcaccg acgtggtcga tatgcacggc
      301 gggcgcgagc acgtcatcgc cctgcgcagc gaccgcaccg tctgggtgtg gggcagcaac
      361 ggcgagggcc agctcgggct cggcaccacg accaaccggc ccacgccgac ccaggtcccc
      421 gggctgagca acgtccaggc cgtcgagacc ggacacaact tgtcgttggc gctcatgggc
      481 gacgggaccg tgcgcacctg gggcctcaac gccgacggtc agctcggcga cggcaccacg
      541 accctgcgac gcagccccgt cacggtctcg gggctcacca acgccgtgtc gatcgcggcc
      601 ggccgcgaca tgagctacgc gatccgtgcg gacggcaccg tggtcggctg gggccgcaac
      661 gacgagggcc agctcggcga cggcaccacg gtgcgccgta cgacgccggt ccgcgtcggc
      721 acgctgacga acgtcatcga catcgccggt ggccgtgacc atgggctggc actgcgcagc
      781 gacggcaccg tgtgggcctg gggctccaac gactacggcc agctcggcga cggcacgacg
      841 accgaccgca ccacgccggt ccaggtgatg agcggtgtga cgcaggtgat cgcgggcgcc
      901 cagcactcct acgcgctgcg cagcgacggc acggtcgccg cctggggtcg caactaccgc
      961 gccaacctcg gtgacggcac gaccaccacc cgcacccggc cggtcaccgt gcgcaaccta
     1021 tcgtcggtcg tctcgatcgg ctcgggccgc gacaccggca tggcggtgct ggccgatggc
     1081 cgggtgatgg cctggggcaa caacacgacc gggcaggtcg gcgacggcac cacgaccaac
     1141 cgcaccaccc cggtcgtcgt acccggagtg tccggcgcgg tgctcgccgc cggtggcggc
     1201 gcggagtact ccgtcgtgct cgtctcgtcc gggccaccgc cgccccagga ccccgtggct
     1261 gccttctctg cgacctgcac ggtcacctcc tgcgacttcg acgccagcgg ctcccacgat
     1321 cccgacggga acctcgtgag ctacgactgg cagttcggcg acaccaccac cggcaccgag
     1381 acggtagcga cgacccacca cgattacgcc acgaccggca cctacagcgt gaccctcacg
     1441 gtcaccgaca actccggcgc cacggcttcg gtcacccacg acgtcgtggc cgaggacacg
     1501 cctcctccgc ccgtcggacc gcagtggcgg gccggagccg cggtggacgc caactcgaac
     1561 cggcccacga tcacgcttcc tgccgccgtg cagtccggcg accggctgct gctggtggtc
     1621 tccgccaacc gggccgccac gctcacgacc ccggccggat ggacgctgct gagcacggcc
     1681 tccgacggca ccgaggtgcg gtcgtgggtg ttcacccggg tcgcca
//
LOCUS       DAORNF010000743         1724 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_49910_length_1724_cov_2.017376, whole genome shotgun sequence.
ACCESSION   DAORNF010000743 DAORNF010000000
VERSION     DAORNF010000743.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1724
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49910_length_1724_cov_2.017376"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="PLV68_20755"
     CDS             <1..263
                     /locus_tag="PLV68_20755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylmuramoylalanyl-D-glutamyl-2,
                     6-diaminopimelate--D-alanyl-D-alanine ligase"
                     /protein_id="HRE03738.1"
                     /translation="DVAGRRVAIVGQMAELADPLADHAGIAAHAAALGIELVAVGTPD
                     YGIDPVADPVAITAALGPGDAVLIKGSLVAGLQRVAAAILAQ"
     assembly_gap    398..415
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<416..1579)
                     /locus_tag="PLV68_20760"
     CDS             complement(<416..1579)
                     /locus_tag="PLV68_20760"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRE03739.1"
                     /translation="MNTGTPPPRRTNDARPGTARAALGYRDFRIIFFGLLASNVGTWM
                     QNLALPAYVDSRTHSATWVAAIGFAQLGPILLLSIPAGVLGDKLPRRQWLITTQMIQL
                     VLALVLAVLIARDANLWAIFSAQLGIGIGSALGGPVMQSSIPLLVSRADLPGALSLNS
                     VMLNGARVVGPALAAVMIAAGLTVSGIFVFNAATYLFAIVAIAIITLPHVVDSGTEQG
                     LAKLFSGVRIARQRSVLSRILLSMTAFSFLCLPYIGLFPTVARLNFGIDSAGSTYKWM
                     FAIWALGGCIGGLSTGVLSLRIDRRRLLVYGYVGFAAVMLAFTFASGPAAALPIAFML
                     GFFYFVIATAMATIFQINMHDHERSRVMSLWMMSFGGTVPIGTGEFRRIHSRVA"
BASE COUNT          322 a          554 c          595 g          235 t
ORIGIN      
        1 tcgacgtcgc cgggcgcagg gtggccatcg tcggtcagat ggccgaactg gccgacccgt
       61 tggcggacca tgccgggatc gccgcccacg ccgccgcgct gggcatcgag ttggtcgccg
      121 tcggcacacc cgactacggc atcgacccgg tggccgatcc ggtggcgatc acggccgcgc
      181 tcggccccgg cgacgccgtg ctgatcaagg gcagcctggt ggcgggtctg cagcgggtcg
      241 ccgccgcgat cctggcccag tagcgcggcc gctagccggg cgtcccttcg gcggccatct
      301 cctcggcggc gatgatgtcg tcgatggtca gtgtctgggc ggggcgacga accaggtccg
      361 tccaccacgc cagcaccaac gaggcgccga cggcgatnnn nnnnnnnnnn nnnnncgcca
      421 cccgcgagtg gatcctgcgg aattcccccg tgccgatcgg caccgtgcca ccgaaggaca
      481 tcatccacag cgacatcacg cgcgaacgct cgtgatcgtg catgttgatc tggaagatgg
      541 tcgccatcgc ggtggcgatc acgaagtaga agaacccgag catgaacgcg atcggcaacg
      601 cggctgcggg acccgatgcg aacgtgaacg cgagcatcac cgcagcaaag cccacatagc
      661 cgtacaccag taggcggcgg cgatcgatgc gcaacgacaa cacacccgtg gagagaccac
      721 cgatgcaacc gcccaaggcc cagatcgcga acatccactt gtacgtcgac ccggcggagt
      781 cgatgccgaa gttgagccgg gcgacggtgg ggaacaggcc gatgtacggc aggcacagga
      841 acgagaacgc ggtcatcgaa agcaggatgc gcgacagcac cgatcgttgg cgggcgatgc
      901 gtactccgct gaacagcttg gccaagccct gttcggtgcc ggagtcgacc acgtgcggca
      961 gcgtgatgat cgcgatcgcg acgatcgcga ataggtaggt ggcggcgttg aacacgaaga
     1021 tgccggacac ggtcaggccg gccgcgatca tcaccgccgc cagcgccggg ccgacgacac
     1081 gggcgccgtt gagcatcacg gagttgaggc tcagtgcgcc gggcagatcg gcccgcgaca
     1141 ccagcagcgg gatcgacgac tgcatcaccg ggccgccgag cgcgctgccg atgccgatgc
     1201 ccagttgggc ggagaagatc gcccacaggt tggcgtcgcg ggcgatcagc acggccagca
     1261 ccaaggccag gacgagttgg atcatctggg tggtgatcaa ccactgtcgc cgagggagct
     1321 tgtcgcccag gactccagcg gggatcgaca gcagcaggat cgggcccagt tgagcgaagc
     1381 cgatcgccgc gacccaggtg gccgaatggg tgcggctgtc gacgtaggct ggcagtgcca
     1441 agttctgcat ccaggtaccg acgttgctgg ccagcagccc gaagaagatg atccggaaat
     1501 cgcggtagcc gagcgcggcc cgcgcagtgc cgggccgggc atcgttggtt cggcgaggtg
     1561 gcggtgtacc ggtgttcatc gatgatccga aagcgactgc agacgttatc ccccagtcgt
     1621 ccctcggctt cagccggacg gtcggccagc cctcaaccga tcagcaggct cgacggatcg
     1681 acgaagtcgg ctccgagcgc ggtcgcgacg ggttcgttga ccac
//
LOCUS       DAORNF010000744         1723 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_49943_length_1723_cov_1.749400, whole genome shotgun sequence.
ACCESSION   DAORNF010000744 DAORNF010000000
VERSION     DAORNF010000744.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1723
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49943_length_1723_cov_1.749400"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(110..487)
                     /locus_tag="PLV68_20765"
     CDS             complement(110..487)
                     /locus_tag="PLV68_20765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03740.1"
                     /translation="MTAVLDAGALIAVDKRDRTVGAMLRVLQREGVPIVTSSAIVAQV
                     WRDPRRQANLARVLAGVDVASLDDTAAQRTGELLRANGTTDLADAHLALLVQPQARVL
                     TSDPSDLAALLATRKVRASLIPA"
     gene            complement(484..>695)
                     /locus_tag="PLV68_20770"
     CDS             complement(484..>695)
                     /locus_tag="PLV68_20770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03741.1"
                     /translation="EIGAAVREAAARAGASVSAWLSEAAAQRLRNDLLGAALDLWEAE
                     DGPFSDDELNAATAALSPARRRGAA"
     assembly_gap    696..712
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            849..>1074
                     /locus_tag="PLV68_20775"
     CDS             849..>1074
                     /locus_tag="PLV68_20775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HRE03742.1"
                     /translation="MDAVEDEFLTTELELSDPAVLSDPAVLRTKTRRYKELTPIVGLI
                     RQHRAASDDAQAARDLLELTDDGDERDLLRD"
     assembly_gap    1075..1100
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1120..>1723
                     /locus_tag="PLV68_20780"
     CDS             1120..>1723
                     /locus_tag="PLV68_20780"
                     /inference="COORDINATES: protein motif:HMM:NF015427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
                     /protein_id="HRE03743.1"
                     /translation="MLLPRDPNDGRAVIVEIRGAEGGEEANLFARDLYEMYVGYAAQQ
                     GWKVEVMSTDVSDLGGINQVTFMISGDTVWSHMKYEGGTHRVQRVPVTESQGRIHTSS
                     ATVMVLAEAEEVDVDIDERDLQIDVYRASGPGGQGVNTTDSAVRITHRPTGIVVAMQD
                     ERSQLQNKARAMTVLRARLLQAAQQEADAKASAERRAQVGS"
BASE COUNT          273 a          512 c          632 g          263 t
ORIGIN      
        1 cgttcgcggt gccgtcgaga tggaacgagg tgttctcggg tccacgctcg catctcggga
       61 tcgctacctg tgtcgacctc gttgcacggt gcccggtggg ctgcgggtgt taggccggga
      121 tgagggaggc tcgtactttt ctggttgcca ggagggcggc gaggtcgctc gggtcgctgg
      181 tgaggactcg ggcttggggt tggacgagca aggccaggtg ggcatcggcc agatcggtgg
      241 tgccgttggc gcggagcagt tcgccggttc gctgggcggc cgtgtcgtcg agcgaggcaa
      301 catcgactcc ggcgaggacg cgagcgaggt tggcctggcg gcgcggatcg cgccacacct
      361 gagcgacgat ggcgctgctg gtgacgatcg gtacgccctc gcgttgcaac acccgcagca
      421 tcgctccgac ggttcggtcg cgcttgtcga ccgcgatcag tgcgcccgcg tcgaggaccg
      481 cggtcatgcg gcgccgcgcc gtcgggcggg actgagcgcg gcggttgctg cgttgagctc
      541 gtcgtcgctg aacgggccgt cctcggcttc ccacaggtcc aacgcggcac cgagcagatc
      601 gttgcggagg cgttgggcgg cggcctcgct gagccaggcc gacacgctgg cacccgctcg
      661 agcggccgcc tcgcggacag cggcaccgat ttcggnnnnn nnnnnnnnnn nnagagccga
      721 tcgacctgtg ccatgccgac gagcgtaccg catacggtcg tatgcgcgga tcggtccgtg
      781 ccctggggag ggggctcgct ggaagatgaa gtggggcggt tagcctgcgg agcgctgtga
      841 tcgatcgttt ggatgccgta gaagacgagt tcctcaccac cgaactggaa ctgagcgatc
      901 cggcggtgtt gagtgatccg gccgtgctgc gcaccaagac gcggcgttac aaggagttga
      961 cgcccatcgt cggactgatc cgtcaacacc gtgctgccag cgatgatgcg caggcggcgc
     1021 gcgacctgtt ggagttgacc gacgatggtg acgagcgcga cctgctgcgc gacgnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn cgacgacgag atcagggtga tgctgctgcc ccgcgatccc
     1141 aacgacgggc gggcggtgat cgtggagatc cgcggggccg aaggcggcga agaagccaac
     1201 ctgttcgccc gcgacctgta cgagatgtac gtcggctacg cggctcagca gggatggaag
     1261 gtcgaggtca tgtccaccga cgtcagcgat ctgggcggca tcaaccaggt gacgttcatg
     1321 atcagcggcg acacggtgtg gagccacatg aagtacgagg gcggcaccca ccgtgtgcag
     1381 cgcgtgccgg tcaccgagag ccagggccgc atccatacgt cgtcggcgac ggtgatggtg
     1441 ttggccgaag cggaagaggt cgacgtcgac atcgacgagc gcgatctgca gatcgacgtc
     1501 taccgcgcca gcgggcccgg cggtcagggc gtcaacacca ccgactcggc cgtgcgcatc
     1561 acccaccgcc cgaccggcat cgtggtggcg atgcaggacg aacgcagcca actgcagaac
     1621 aaggcacggg cgatgaccgt gctgcgtgcc cgcctgctgc aggccgcaca gcaggaggcc
     1681 gatgccaagg ccagcgccga gcggcgggcg caggtcggtt cgg
//
LOCUS       DAORNF010000745         1723 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_49947_length_1723_cov_1.483813, whole genome shotgun sequence.
ACCESSION   DAORNF010000745 DAORNF010000000
VERSION     DAORNF010000745.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1723
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49947_length_1723_cov_1.483813"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="PLV68_20785"
     CDS             <1..271
                     /locus_tag="PLV68_20785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03744.1"
                     /translation="SSGATVGGAQLLIAGSVRQYSTFAFSAHTDDTEAPPLRQVLLGQ
                     PSSPARGVIRVQLGNFEVTVPAALKPGFASQMVIFENVGEDNVHF"
     gene            289..>803
                     /locus_tag="PLV68_20790"
     CDS             289..>803
                     /locus_tag="PLV68_20790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03745.1"
                     /translation="MRNSLSRSLARGAAFAMTLGAATQVSANPTAGVDVALFRASYDT
                     GGIFSLEGARLMPKRDISWKMLLSYAKSPVDVAVPGIGGADDTSADSILDYLAIVDIA
                     FGMALSERFSIGFDVAAYRTDTGAGYGKRGLYGGSAGNMPSTGLVALRPLSNIDPAGG
                     TLGDGRAGPLDA"
     assembly_gap    804..876
                     /estimated_length=73
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <877..>1723
                     /locus_tag="PLV68_20795"
     CDS             <877..>1723
                     /locus_tag="PLV68_20795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03746.1"
                     /translation="GEDEMLLGDSSLTFEPKLAFDYRFDRVRATKVVVNVGARLRKRT
                     VLESFDITAADPSAKAFLDVGSEALAGAGMIYELSPRVLLAAEGTAFIPLPNSMSLGG
                     CRLSTGRECDAMSDTDYFADTKAGDLAVLANAGAQFRINEHVTGSVVAGMGPVGARGD
                     DFRITTGIVWSPQPVGSASVGRADVDDDGVPDSIDACVGEAEDRDGFQDEDGCPELDN
                     DNDGIPDDQDKCVDELEDRDGFQDEDGCPETDNDGDQVADAADRCPDEKEDIDGFDDA
                     DGCPDL"
BASE COUNT          259 a          564 c          555 g          272 t
ORIGIN      
        1 atctagcggg gccacggtcg gtggcgctca gctcctgatc gccggcagcg ttcgccagta
       61 ctcgaccttc gcgttctccg cccacaccga tgacaccgag gcgccgccgc tgcgccaggt
      121 gctgctcggg cagccaagca gcccggcgcg cggcgtgatc cgcgtccagc tcggcaactt
      181 cgaggtcacc gttcctgccg cgctcaagcc tggctttgcg tcgcagatgg tcatcttcga
      241 gaacgtcggc gaagacaacg tgcacttctg atgcgaggcc aactctccat gcgcaacagc
      301 ctctctcgct cgctcgcacg cggcgcagcg ttcgcgatga ccctcggcgc ggcgacgcag
      361 gtgtcggcca accccaccgc gggcgtcgac gtcgcgttgt tccgcgcgtc ctatgacacc
      421 gggggcatct tctcgctcga gggcgcgcgc ctcatgccca agcgcgacat ctcctggaag
      481 atgctccttt cctatgccaa gtcgccagtc gacgtggcag tgccaggcat cggcggcgcc
      541 gacgacacct ccgccgactc gatcctggac tacctcgcca tcgtcgatat cgcctttggg
      601 atggcgctca gcgagcggtt ctcgattggc ttcgacgtgg ccgcgtaccg caccgacacc
      661 ggcgctggct acggcaagcg aggtctctac ggcggctcgg ccggcaacat gccatcgacc
      721 ggtttggtgg cgctgcggcc gctgtccaac atcgacccgg cgggcggcac cctcggcgat
      781 ggccgtgccg ggcctctcga tgcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntcgg cgaggacgag atgctgctag
      901 gagactcgag cctgacgttc gagcccaagc tggcgttcga ctatcgcttc gatcgcgtac
      961 gcgccaccaa ggtcgtggtg aacgtgggcg cgcgcctgcg caagcgcacg gtgctcgaga
     1021 gcttcgatat caccgctgcc gatccttcgg ccaaggcgtt cctggacgtc ggctcggaag
     1081 cgttggccgg ggccggcatg atctacgagc tgtccccgcg cgtgctcctg gctgccgagg
     1141 gcacggcgtt catccctctg ccgaactcga tgtcgctcgg cggctgccgg ttgtcgaccg
     1201 gtcgcgagtg cgatgccatg agcgacaccg actacttcgc tgataccaag gccggcgacc
     1261 tggcggtgct ggccaacgcg ggcgcgcagt tccgcatcaa cgagcacgtg accggctcgg
     1321 tggtggcggg catggggccc gttggcgcgc gcggcgacga ttttcggatc accaccggga
     1381 tcgtgtggtc gccgcagcca gtgggcagcg cttccgtggg gcgcgccgat gtcgacgacg
     1441 atggcgtgcc cgacagcatc gacgcctgcg ttggcgaggc cgaagatcgc gatggcttcc
     1501 aggacgagga cggctgccca gagctcgaca acgacaacga cggcatcccg gacgaccagg
     1561 acaagtgtgt cgacgagctg gaagatcgcg atggcttcca ggacgaggac ggctgcccgg
     1621 agaccgacaa cgacggcgat caggtcgccg acgcggcgga ccgttgcccc gatgagaagg
     1681 aagacatcga cggcttcgat gacgccgacg gctgcccgga tct
//
LOCUS       DAORNF010000746         1721 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_50005_length_1721_cov_1.460384, whole genome shotgun sequence.
ACCESSION   DAORNF010000746 DAORNF010000000
VERSION     DAORNF010000746.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50005_length_1721_cov_1.460384"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1529
                     /locus_tag="PLV68_20800"
     CDS             <1..1529
                     /locus_tag="PLV68_20800"
                     /inference="COORDINATES: protein motif:HMM:NF025078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HRE03747.1"
                     /translation="PDRMTVGTEWVSAAPGSLFASRDGAGPLHRRARRRVEITDGVVA
                     RTLAPGHLANGAMALPIASSRALPARVDREAVVGTADAADLSVPDLSVAGLSVADLSV
                     AGSDVHNVPQPLLAPQSSSDATTDRSAATEPGKANKATKAAKAAKSKQSATPEHLANT
                     AEAAEVAATPHVDLATSAVRPAIARETASETPSERAPDDAATAPATATARDGAGRYGP
                     GPRIAATGLADAFLTELSRHRDEAPRPLPVQFASIAELIVPNRRPLLSTSAASRAALA
                     SVGKVAATVGDVIHLAEAPAASRPSAELMGVVAHELTHVAAPSAAPRFFDDDRDTPEE
                     RRAFEIGELIRRSPVLPSRAIARQTASPPGDETSNLAGGPSRAPTAITQTGPTTGSGS
                     GTGAGSLTEQLLASRLGVTNGLVQRGSLDTVSSSGTVTSSPPNDPFTHQSGSDTGASQ
                     MPESSTQGSDPSGSSLVNIDADTFERHLRDHFDLIVDLLEDRIVAELDRRGGRFRGDF
                     "
     gene            1529..>1721
                     /locus_tag="PLV68_20805"
     CDS             1529..>1721
                     /locus_tag="PLV68_20805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03748.1"
                     /translation="MAPNSPSPAGSSNAPAQLQKAFLETETGERIPCMFNPDKFSFST
                     SNRWEADNLPGLIADVLGNR"
BASE COUNT          273 a          662 c          546 g          240 t
ORIGIN      
        1 gtcccgaccg gatgaccgtc ggcaccgagt gggtgtcggc cgcccccggc tcgctgttcg
       61 ccagccggga cggcgcggga cctctccacc gccgtgcccg tcggcgcgtc gagatcaccg
      121 atggtgtggt ggcgcgcacg ctcgcgcccg gccatctcgc caacggcgcg atggcactgc
      181 cgatcgcaag ctcgcgggcg ctgcctgccc gtgtcgaccg cgaggctgtc gtcggcaccg
      241 ccgacgcggc cgatctcagc gtgcccgatc tcagcgtggc cggtctcagc gtggccgatc
      301 tcagcgtggc cggctccgac gtacacaacg tgccgcagcc gttgctcgca ccgcaatcga
      361 gcagcgacgc caccaccgat cggtccgcag caaccgagcc aggcaaggcc aacaaggcga
      421 ccaaggccgc caaggccgcc aagtccaagc agtcggccac gcccgaacac ctcgccaaca
      481 cggccgaggc ggccgaggtt gcggcgacac cgcatgtcga tctggcgacc tcggcggtgc
      541 gtcctgccat cgcccgcgag acggcctccg agacgccctc cgagagggcg cctgacgatg
      601 cagccactgc gccagcgaca gcgacggcgc gtgacggcgc cggtcgatac gggccgggtc
      661 cgcgcatcgc tgccaccggg ctggccgatg cgttcttgac cgagctcagc cgccatcgcg
      721 acgaggcacc gcgaccgctg ccggtgcagt tcgcaagcat cgccgaactg atcgtgccca
      781 accgtcgtcc gctgctctcg accagcgccg cgtcacgcgc tgcgctggcc agcgtgggca
      841 aggtcgcggc cacggtgggc gacgtgatcc acctggccga agcaccggcc gcgagccgtc
      901 cttcggcgga actgatgggt gtggtcgcgc acgagctcac ccacgtcgcc gctccctcgg
      961 ccgcgccgcg gttcttcgac gacgatcgtg atacgcccga agaacgccgg gcgttcgaga
     1021 tcggtgagct gattcgccgt tcgcccgtgc tgcccagccg cgcgatcgcc cgccagaccg
     1081 ccagccctcc tggcgacgag accagcaatc ttgctggcgg cccgtcccgc gcaccgactg
     1141 ccatcacaca gaccggcccg accactggca gtggatccgg caccggcgcc ggctcgttga
     1201 ccgaacagct cttggcttcg cgccttggcg tcacgaacgg gctcgtccag cgtggctcgc
     1261 tcgacacggt cagttcgtcg ggtacggtca ccagtagtcc gcccaacgat ccgttcaccc
     1321 atcagtcggg gtccgacacc ggagcaagcc agatgcccga atcatccacc caggggtcgg
     1381 acccgtccgg ttcgagcctc gtcaacatcg acgccgacac gttcgagcgt cacctccgcg
     1441 accacttcga cctcatcgtc gatctgctcg aggaccgcat cgtcgccgaa ctcgaccgcc
     1501 gcggcggccg cttccgagga gacttctgat ggcccccaac tcacccagcc cggccgggtc
     1561 gagcaacgcc ccggcgcaac tgcagaaggc gttcctcgag accgagacgg gcgagcgcat
     1621 cccgtgcatg ttcaaccccg acaagttcag cttctccacg tcgaaccggt gggaggccga
     1681 caacctgccc ggtctcattg ccgatgtgct cggcaaccga c
//
LOCUS       DAORNF010000747         1719 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_50055_length_1719_cov_1.596154, whole genome shotgun sequence.
ACCESSION   DAORNF010000747 DAORNF010000000
VERSION     DAORNF010000747.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50055_length_1719_cov_1.596154"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..486
                     /locus_tag="PLV68_20810"
     CDS             <1..486
                     /locus_tag="PLV68_20810"
                     /inference="COORDINATES: protein motif:HMM:NF013431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03749.1"
                     /translation="ACPLGPDWPFGGVATQRITLDADHLRAELAVTAGNIAMPAQVGW
                     HPWFWKPGSADLRFDAMYQRDSDGIPTGELVAPPAGPADDCFVRPLGPLQLHHDEVTI
                     TIDSDCDHWVVYSQQRRELCVEPQSGPPDGFTLAPFVLQPGETLRRWMTIGWSAVAGL
                     R"
     gene            556..>1386
                     /locus_tag="PLV68_20815"
     CDS             556..>1386
                     /locus_tag="PLV68_20815"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRE03750.1"
                     /translation="MSTASDPNGPLRIAYLTYRGKPHVGGQGVYTRHLTKALVDLGHE
                     VEVFAGQPYPIVDERIPLHKLPSLDIFNDHYPGRLPAYWEIKSKEDLLETAQYMTGTF
                     SEPLAFSARAYKELKRRTRDFDLVHDNQCLGYSILQIEKIIPTIVTLHHPITRDRALE
                     MAHAPTRRKRRSVGRWYGFVKMQGRVASRMPRIVVVSENSIKDIHNDMGVSLDRMRLV
                     PVGVDPDLFQPRPDVTRIPGRLITTASADVALKGLSYLLEAMAKLRTERDITLTIIGK
                     P"
     assembly_gap    1387..1428
                     /estimated_length=42
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1429..>1719
                     /locus_tag="PLV68_20820"
     CDS             <1429..>1719
                     /locus_tag="PLV68_20820"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRE03751.1"
                     /translation="RPHINFVSGVSDERIVELYAESELAVVPSLYEGFSLPAIEAMCT
                     GTPLVATDGGALPEVTGADGETVFQCPAGDAGALAARIGAALDDPALRARVGA"
BASE COUNT          290 a          608 c          530 g          249 t
ORIGIN      
        1 gcgtgtccac tcggccccga ttggcccttc ggcggtgtcg cgacccaacg gatcacgctc
       61 gacgccgacc atctccgggc cgagttggcg gtcaccgcag gcaacattgc gatgcccgcc
      121 caagtcggtt ggcacccatg gttctggaag ccgggcagcg ccgatctgcg tttcgacgcg
      181 atgtaccaac gcgacagcga cggcattccc accggcgaac tggtcgcccc accagccgga
      241 ccggcggacg actgcttcgt ccgtccgctc gggccgctgc aactgcacca cgacgaggtc
      301 acgatcacga tcgacagcga ctgcgaccac tgggtggtgt actcccagca acgacgggag
      361 ctgtgcgtcg aaccccagag cggcccaccc gacggcttca cgctcgcccc gttcgtgctg
      421 caacccggcg agacgctgcg gcggtggatg acgatcggct ggtctgctgt ggctggtctg
      481 cggtgaggct ggccggttcg ggcgcgtcaa ccccggctac tgtccggtaa cgaagcccag
      541 atagcgtcag tcctcatgtc aacagcgagc gaccccaacg ggcccctccg catcgcgtat
      601 ctgacctacc gcggcaaacc ccacgtcggc ggccagggcg tgtacacccg ccatctcacc
      661 aaggcgttgg tcgatctcgg ccacgaggtg gaggtgttcg cgggccagcc ctacccgatc
      721 gtcgacgagc ggataccgct gcacaagctg cccagcctcg acatcttcaa cgaccactac
      781 ccggggcgcc tgcccgccta ctgggagatc aagagcaagg aagacctgct cgaaaccgcg
      841 cagtacatga ccggcacgtt cagcgagccg ctcgccttca gcgcccgtgc gtacaaggag
      901 ctgaagcggc gtacccgcga tttcgacctg gtgcacgaca accagtgcct cggctacagc
      961 atcttgcaga tcgagaagat cattcccacc atcgtcaccc tgcaccaccc gatcacccgc
     1021 gaccgcgcgc tcgagatggc gcacgccccc acccggcgca agcggcgtag cgtcgggcgc
     1081 tggtacgggt tcgtcaagat gcagggccgc gtcgccagcc ggatgccgcg catcgtcgtg
     1141 gtcagcgaga acagcatcaa ggacatccac aacgacatgg gcgtcagcct cgaccggatg
     1201 cggttggtgc ccgtcggcgt cgaccctgac ctgttccagc cgcggcccga cgtgacccgc
     1261 atccccggcc ggttgatcac caccgcgtcg gccgatgtgg cgctgaaggg cctgtcgtac
     1321 ctgctcgaag cgatggccaa gctgcgcacc gagcgcgaca tcacgctgac gatcatcggc
     1381 aagcccnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngc gcccgcacat
     1441 caacttcgtc agcggagtca gcgacgaacg gatcgtcgag ctctacgccg agtccgagtt
     1501 ggccgtggtc cccagcctgt acgaggggtt cagcctgccg gcgatcgaag cgatgtgcac
     1561 cggcacaccg ttggtcgcca ccgacggtgg ggcgctgccc gaggtcaccg gcgcagacgg
     1621 tgagacggtg ttccagtgcc cggctggcga cgccggagcg ctcgcggcac gcatcggtgc
     1681 ggccctcgac gaccccgccc tgcgcgcccg cgtcggcgc
//
LOCUS       DAORNF010000748         1716 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_50142_length_1716_cov_1.862131, whole genome shotgun sequence.
ACCESSION   DAORNF010000748 DAORNF010000000
VERSION     DAORNF010000748.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1716
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50142_length_1716_cov_1.862131"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..583)
                     /locus_tag="PLV68_20825"
     CDS             complement(<1..583)
                     /locus_tag="PLV68_20825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HRE03752.1"
                     /translation="MTNSDTPRRDEPPRRDDPPRRDDPWLMRTYSGHSTAQASNELYR
                     ANLAKGQTGLSIAFDLPTQTGYDPDSPFARGEVGKVGVPVAHLGHMRTLLDQIPPGQM
                     NTSMTINAPAAWLLALYVANAAEQGVESHALRGTTQNDIAKEYLSRGTYIFPPEASRR
                     LIVDMIAWCADNAPKWNPMNVCSYHLQEAGATPV"
     gene            complement(740..1414)
                     /gene="aat"
                     /locus_tag="PLV68_20830"
     CDS             complement(740..1414)
                     /gene="aat"
                     /locus_tag="PLV68_20830"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HRE03753.1"
                     /translation="MFATAPIEPPPTQWRFPPAFRADKQGLVAVGADLEPGTLLTAYR
                     SGLFPMPVSKRAIGWWSPNPRGVLPLDGLRITKSLRQSRRRYRVEVDTCFSEVMRRCA
                     DPSRPGGWITHDFIAAYSRLHALGWAHSVETYDEHGALVGGLYGVRIDGLFAGESMFS
                     TARDASKVALAELVEQLRAVGVTLLDVQWTTPHLVSLGARDIGRDEYLRLLDVAVGNT
                     GRSIAD"
     gene            complement(1461..>1716)
                     /locus_tag="PLV68_20835"
     CDS             complement(1461..>1716)
                     /locus_tag="PLV68_20835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HRE03754.1"
                     /translation="IRLAELGTASIADIDAAMKGGCNFPLGPFFTLDLVGLDTSLAIL
                     DALYAEFGDPNYVAVPRLRRMVAAGLLGRKSGRGFYDYSR"
BASE COUNT          271 a          559 c          617 g          269 t
ORIGIN      
        1 gcaccggcgt cgcgcccgcc tcttgcaggt ggtacgagca gacgttcatc gggttccact
       61 tgggcgcgtt gtcggcgcac caggcgatca tgtcgacgat caaccgccgc gacgcctcgg
      121 gcgggaagat gtacgtaccg cggctgaggt actccttggc gatgtcgttc tgggtggtgc
      181 cgcgcagcgc gtgcgactcg acgccctgct cggcggcgtt ggcgacgtac agcgcgagca
      241 gccacgcggc gggcgcattg atcgtcatcg acgtgttcat ctgccccggt gggatctggt
      301 cgagcagcgt gcgcatgtgg cccagatggg cgaccggcac cccgaccttg ccgacctcgc
      361 cgcgggcgaa ggggctgtcc gggtcgtatc cggtctgggt cggtaggtcg aacgcgatcg
      421 acagccccgt ctggcccttg gccaggttgg cccgatagag ctcgttcgag gcctgggccg
      481 tggagtggcc cgagtaggtg cgcatcaacc acggatcgtc gcgacgcggg ggatcgtcgc
      541 gacgcggggg ctcgtcccgg cgtggggtgt cgctgttcgt catcgtcccc attgttaccc
      601 gcgggtagtc aggcccgcga gtttttcaag aacttctcaa gtcgcccacg caggtgccga
      661 tgactgtcat cgcacaaggc gggacgtgcc agcgggtgga tcgacggcgt cggagtggcg
      721 gaactccggc gccgacggtt cagtccgcaa tcgatcgacc ggtgttgccg accgcgacgt
      781 cgagcaggcg caggtactcg tcgcggccaa tgtcgcgagc accgagcgag acgaggtgcg
      841 gcgtggtcca ctgcacgtcg agcagcgtga cgcccaccgc acggagctgc tcgaccagtt
      901 cggccaacgc gaccttcgac gcgtcacggg ccgtggagaa catgctctcg ccggcgaaca
      961 gtccgtcgat gcgcacgccg tagagaccgc cgaccaacgc gccgtgctcg tcgtaggtct
     1021 ccacgctgtg ggcccagccc agcgcatgca ggcgggagta ggcggcgatg aagtcatggg
     1081 tgatccaacc gcccggccga ctgggatcgg cacaccggcg catgacctcg ctgaagcacg
     1141 tgtcgacctc gacccgatag cgccgccggc tctggcgcag cgacttggtg atgcgcaaac
     1201 cgtcgagcgg taggaccccg cgcgggttgg gtgaccacca cccgatcgcc cgcttgctga
     1261 ccggcatcgg gaacaacccc gagcggtagg cggtcagcag ggttcccggt tcgaggtcgg
     1321 ctccgacggc gaccaggcct tgcttgtcgg cacggaatgc cggggggaaa cgccactgtg
     1381 tcgggggcgg ttcaatgggc gcagtggcga acatgcgccg cccagtctgg ccgaccagcg
     1441 ccctgggtca gccagcgcga tcagcgtgag tagtcgtaaa agccccggcc ggacttgcgg
     1501 ccgaggagac ctgcggcgac catgcgccgc agcctcggca ccgccacgta gttcgggtcg
     1561 ccgaattcgg cgtagagcgc gtcgaggatc gccagcgagg tgtcgagccc gaccaggtcg
     1621 agcgtgaaga aggggcccaa cgggaagttg cagccaccct tcatcgccgc gtcgatgtcg
     1681 gcgatgctcg cggtacccag ctcggcgagg cggatc
//
LOCUS       DAORNF010000749         1712 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_50302_length_1712_cov_1.082076, whole genome shotgun sequence.
ACCESSION   DAORNF010000749 DAORNF010000000
VERSION     DAORNF010000749.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50302_length_1712_cov_1.082076"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    230..383
                     /estimated_length=154
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<384..656)
                     /locus_tag="PLV68_20840"
     CDS             complement(<384..656)
                     /locus_tag="PLV68_20840"
                     /inference="COORDINATES: protein motif:HMM:NF019428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HRE03755.1"
                     /translation="MSPHRRDHGQSTVELALAMPLVCMLLLAVVQLAVVVRDRLAVAD
                     AARVGARAAAVSANPSAAGSSAALGALGAAVGPGRAVVGVHGQLVTV"
     assembly_gap    755..850
                     /estimated_length=96
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(931..>1390)
                     /locus_tag="PLV68_20845"
     CDS             complement(931..>1390)
                     /locus_tag="PLV68_20845"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HRE03756.1"
                     /translation="ITAAYPDFVDLLVLTIHAGRTPSQAVRALSSTLDGPLGAALAAV
                     TSEVDHGQRFDVALAALVASLGPVAQPVVDALTLAMRAGVPLAATLDRLAVDAHAQRR
                     RAAEASARQLPVRLSFPLVGLTLPSFVVLAIVPLLAGTLSSLGGLSPHTP"
     assembly_gap    1391..1432
                     /estimated_length=42
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          251 a          498 c          514 g          157 t
ORIGIN      
        1 caactccacc cgcccaccgt accggtcgaa gcaacagtgt tcagtcgacc cgcgcagcgg
       61 gggcgaatcg aacacacttg gccgcccggc ccgggactgc gttcagttga cccgcgcagc
      121 gggggcgaat cgaacacacc cggccgcccg gcccgggact gcgttcagtt gacccgcgtg
      181 gaaggggcga atcgaacaca cccggggcga cagggcgggg tcatgggggn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnncaccgtc accaactgac cgtggacgcc gaccaccgct
      421 cgaccggggc cgaccgctgc gcccagcgcg ccgagcgcgg cggagctgcc cgccgcgctg
      481 gggttggccg acacagccgc ggcccgcgcc ccgactctgg ctgcgtcggc gaccgcgagc
      541 cggtcgcgca cgacgacggc cagttgaacc acggcgagca gcagcatgca caccagcggc
      601 atcgccaacg ccagctcgac cgtgctctgc ccgtgatccc gacggtgagg actcacacgc
      661 ggctggtgac ttcgtcgagg accttgtcga acaggcggcc gatcttgccc gcgccgccac
      721 cggcggtagc ccaggcgacg acgagcaggg cgatnnnnnn nnnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      841 nnnnnnnnnn aggcggatga aggagcggct catgatggac tcctgacgag ggtcggacga
      901 gacgtggacg agtgagaggt ccgtgcccgc tcagggcgtg tgggggctga gcccaccgag
      961 ggacgacaag gtgcccgcca gcaagggaac gatggcgagc acgacgaaag acggcagggt
     1021 caaaccgacc aaggggaagg agaggcgcac gggcagttgg cgagcagacg cttcggccgc
     1081 gcgacggcgc tgtgcatgcg cgtcgacggc gagtcgatcg agtgtggctg cgagcggaac
     1141 gccggcacgc atcgccaggg tcagcgcgtc gaccaccggc tgggcaaccg ggccgagcga
     1201 tgcaaccagg gctgccagcg cgacatcgaa gcgctgcccg tgatcgacct cggaggtcac
     1261 cgcggcgagg gctgcgccga gcggtccgtc gagcgtcgaa ctcagggcgc gcaccgcctg
     1321 gctcggcgtg cgtccggcgt gaatcgtgag caccagcaga tcgacaaagt cggggtaggc
     1381 cgccgtgatc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnatccaccg
     1441 ggtcacgtac ggcaccaccg cggccgcagc cgcgcccatc gggccgacgg caacagcgac
     1501 caccgccagt gcgacgacca tggcaccgcg ccgcggccag tgctggcgcg gtcgcgtccg
     1561 ttccgtggct gagggcgtct tcgtgcagac ccgaggaacg gcatggcgcg gccgcgtgac
     1621 gaatgcaccg atgacgcaca gccccgacgc gcatgctgcc gtgatcgtcg cagccgcgct
     1681 catctcgcgt cccccgcgac accgattcga cc
//
LOCUS       DAORNF010000750         1712 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_50303_length_1712_cov_1.049487, whole genome shotgun sequence.
ACCESSION   DAORNF010000750 DAORNF010000000
VERSION     DAORNF010000750.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50303_length_1712_cov_1.049487"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>239
                     /locus_tag="PLV68_20850"
     CDS             <1..>239
                     /locus_tag="PLV68_20850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03757.1"
                     /translation="VGLVATSSSSSSAKKVAKLAQRGKGKKVRFQGGTLFPAVIVGVL
                     VLLLPLIIYARESRPSAGEGEPTINQHWHAAFAMY"
     assembly_gap    240..450
                     /estimated_length=211
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <451..>667
                     /locus_tag="PLV68_20855"
     CDS             <451..>667
                     /locus_tag="PLV68_20855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03758.1"
                     /translation="NSKLELPAEQAPPDGRTFEEGKTKCVVDGQEKPASIKAWVWDNY
                     SQVGVSDPSVYTADIGGIRIRNDGMVVV"
     assembly_gap    668..761
                     /estimated_length=94
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            922..>1712
                     /locus_tag="PLV68_20860"
     CDS             922..>1712
                     /locus_tag="PLV68_20860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HRE03759.1"
                     /translation="MRAVVLVGGFGTRLRPLTLSVPKPMLPVGHVPIIQRLIANLVRG
                     GVVDVTLALGFKPEPFIAAFPDGTCEGARLHYAVEPEPLDTAGAIRFAALHAGIDDTF
                     VIANGDVLTDLDVAALVGFHRQRGAEATLHLTPVEDPSGFGVVDCDDEGRVRRFVEKP
                     APGTAPSNRINAGTYVFEPSVIERIADGRRVSVERETFPAIVADGGLWAMATDDYWVD
                     TGRPDLYLQANLDLINGRRADGLDPAEAVGARAVIGADVAITNSI"
BASE COUNT          222 a          481 c          478 g          226 t
ORIGIN      
        1 gtgtcggact cgtggcaacc tcgtcatcgt catcgtccgc caagaaggtc gcgaagctcg
       61 cgcagcgcgg caagggcaag aaggtccgct tccagggcgg caccttgttc ccagccgtca
      121 tcgtcggtgt gttggtgctg ctgctgccgc tcatcatcta cgctcgcgag agccgtccca
      181 gcgctggcga gggcgagccg accatcaacc agcactggca cgcggcattc gcgatgtacn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn caactcgaag ttggagttgc ccgccgagca
      481 ggccccgccg gatggtcgga ccttcgaaga gggcaagacc aagtgcgtcg tcgacgggca
      541 agagaagccg gcctcgatca aggcgtgggt gtgggacaac tactcccagg tcggcgtcag
      601 tgatcccagc gtgtacaccg ccgacatcgg tggtatccgc atccgcaacg acggcatggt
      661 ggtcgtgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncaccaccag cactggtgag
      781 tccgtgacgc ccgccggcga cgagaacgac gtgaccgttc ccagcgtcgc gacctcgtcc
      841 agcgacgcaa cgccctccag cgacgtgacg tcgccgacgg cggatgcacc cacgtccacc
      901 gcgggtagct gaccaccggc catgcgcgcc gtcgtcctcg tcggcgggtt tggaacccgc
      961 ttgcgtccgt tgacgctgtc ggttcccaag ccgatgctgc cggtcggtca cgtaccgatc
     1021 atccagcggt tgatcgccaa cctggtccgc ggcggcgtcg tcgacgtgac gttggcgctc
     1081 ggcttcaagc ccgagccgtt catcgccgcg tttcccgacg gcacctgcga gggtgcgcgc
     1141 ctgcactatg cggtcgagcc cgagccgctc gataccgccg gtgcgatccg cttcgcggcg
     1201 ctccacgcgg gcatcgacga cacgttcgtg atcgccaacg gtgacgtgct gaccgacctc
     1261 gacgtcgccg cgctggtcgg cttccaccgc cagcgtggcg cggaggcgac gctgcatctg
     1321 acgccggtcg aggacccgtc gggcttcggc gtggtcgact gcgacgacga gggtcgtgtg
     1381 cgccgtttcg tcgaaaagcc ggctccgggc accgcgccga gcaaccgcat caacgcgggg
     1441 acgtacgtgt tcgagccgag tgtgatcgag cgcatcgccg atgggcgcag ggtgtcggtc
     1501 gagcgggaaa cgttcccggc gatcgtggcc gacggcgggc tgtgggcgat ggcgaccgac
     1561 gactactggg tcgacaccgg tcgtcccgac ctgtatctgc aggccaacct cgatctcatc
     1621 aacggccgcc gcgccgacgg gctcgacccg gccgaggcag tcggtgcgcg ggcggtgatc
     1681 ggcgccgacg tggcgatcac caactcgatc at
//
LOCUS       DAORNF010000751         1709 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_50408_length_1709_cov_1.954051, whole genome shotgun sequence.
ACCESSION   DAORNF010000751 DAORNF010000000
VERSION     DAORNF010000751.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50408_length_1709_cov_1.954051"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..754)
                     /locus_tag="PLV68_20865"
     CDS             complement(<1..754)
                     /locus_tag="PLV68_20865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HRE03760.1"
                     /translation="MIVVVLAGAYLATAKIVGLVPFASTDDAEAVETAASTQQLVAVS
                     EGTIAETVSAESSVVAAATESLSFASSGKVAEVLVAEGDVVTAGQLLARLDSAALRAD
                     FAEAQASAASLEAQLENSMVELWEYREDNPDEDDTYDDGDLLEDQIASTQARLAVQYD
                     TLADAMIALAGADLVASIDGTVTTVDLTVGEQLGSGGASGTSMTGSGSGSGNSSGSIG
                     SGAGAFPGQSSAGGSTSTGQIEIVSTGRYQVEL"
     gene            1116..>1709
                     /locus_tag="PLV68_20870"
     CDS             1116..>1709
                     /locus_tag="PLV68_20870"
                     /inference="COORDINATES: protein motif:HMM:NF015534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HRE03761.1"
                     /translation="MRMLLTSNGLTNPTIRDCFDELLGKPTAESRIAVVIDAFMGFPH
                     DKGGLLDHLQHYRGFGWANFDVISLLAAPRPLVEARLRSADVIYCYGGSNHWLAHAWR
                     ESGLAPVLAELLDDKVYLGMSDGSMIFSRAHAATVDAFNNRDELTMFGLDAAEPAVAL
                     FDWYVIGHLGSEFLPEQTDEWATRTAARLGSDVWYLDD"
BASE COUNT          264 a          607 c          569 g          269 t
ORIGIN      
        1 agagctcgac ctgatagcga ccggtgctga cgatctcgat ctgtccggtc gacgtcgagc
       61 cgcccgctga cgactggccg gggaacgccc ccgccccgga gccgatcgag cccgacgagt
      121 tgcccgatcc gcttcctgag ccggtcatcg aggtgccgct cgcaccaccg ctgccgagct
      181 gctcaccgac ggtgagatcg acggtggtca cggtgccgtc gatcgacgca accagatcag
      241 ccccggcgag cgcgatcatc gcgtcggcga gcgtgtcgta ctgcacggca aggcgcgcct
      301 gggtggaggc gatctggtct tccagcagat cgccgtcgtc gtaggtgtcg tcctcgtccg
      361 ggttgtcctc gcggtactcc cacagctcga ccatggagtt ctcgagctgc gcctccagcg
      421 acgcggccga cgcctgggct tcggcgaagt cggcgcgcag cgcggccgag tccaaccggg
      481 ccaacaactg tcccgctgtg accacgtctc cctcggcgac gaggacctcg gccactttgc
      541 ctgacgaggc gaacgacagc gactcggttg cggcggcgac gaccgacgac tcggccgaca
      601 cggtctcggc gatcgtgccc tcgctcaccg ccacgagctg ctgcgtgctg gccgcggtct
      661 cgaccgcttc ggcgtcgtcc gtgctcgcga acgggaccag accgacgatc ttcgccgttg
      721 cgagatacgc gccggccagc acgacgacga tcaccgcggc gatcaggaat cgcttgcgcc
      781 gacggcgccg cggcgctggg ttcggggccg gggctggacc gtcagcgtga ggaccagtcg
      841 agacgcgcgg ctcatcgagc accgctgagg tcgatggcat gagatctcct gtcgtgttcg
      901 gggacgagcc gcgggctgcg cggcattgct cccgcacggt agaaatcgaa gctgagaagt
      961 ccctggcaag tcggcaggac ctcgccaaga gttcacggcg cccaggcccg gtggcccacg
     1021 ttgctcggcg cacgctgctc ggcggcgcgc gccggtgacg caccacgccc gaccgcgcag
     1081 ctcagcgtga cgcggcgatc ggcaaggatg aggcgatgcg gatgctgttg acctcgaacg
     1141 gattgaccaa cccgacgatc cgggactgct tcgacgagtt gctcggcaag ccgaccgccg
     1201 agtcgcggat cgccgtcgtg atcgacgcgt tcatgggctt cccgcacgac aagggcggcc
     1261 tgctcgatca tctccagcac taccgcgggt tcggctgggc caacttcgac gtgatctcac
     1321 tgctcgccgc tccacgcccg ctggtcgagg cccggctgcg gtccgccgac gtcatctact
     1381 gctacggcgg cagcaaccac tggttggccc acgcgtggcg cgagagcggc ctcgcccccg
     1441 tgctggccga gttgctggac gacaaggtgt acctcggcat gagcgacggg tcgatgatct
     1501 tctctcgcgc tcacgccgcc acggtcgacg cgttcaacaa tcgcgacgag ctcaccatgt
     1561 tcggcctcga cgcggccgaa cccgccgtgg cactattcga ctggtacgtc atcggccatc
     1621 tcggctcgga gttcctcccg gaacagaccg acgagtgggc cacccgcacc gccgcccgcc
     1681 tcggctcaga cgtgtggtac ctggacgac
//
LOCUS       DAORNF010000752         1709 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_50411_length_1709_cov_1.827690, whole genome shotgun sequence.
ACCESSION   DAORNF010000752 DAORNF010000000
VERSION     DAORNF010000752.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50411_length_1709_cov_1.827690"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>820
                     /locus_tag="PLV68_20875"
     CDS             <1..>820
                     /locus_tag="PLV68_20875"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HRE03762.1"
                     /translation="HPEAFPPYYMGILESAELTGRLDETLESLADYLERELDTRSKVV
                     SALSYPGVVMVMALFTVAVLAGYVLPQFKPLFEEMNADLPLATRSLLFFADVFTIYWY
                     IPLTGFICMFAGVLWLTKTEKGKRVKDKLVLKIPVVGGIIDYAILERFCRILSTMIRA
                     GVPLPVGLRTTTEATSNTIYRERLEIAQAEMLEGGGFSKPLVETGLFPGAAKQMFKVG
                     EETGTLDEQLAVASTYFDRELESRIKKFTTMFEPIMIVGVGVMVGFVAVALVSAM"
     assembly_gap    821..867
                     /estimated_length=47
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(893..>1709)
                     /locus_tag="PLV68_20880"
     CDS             complement(893..>1709)
                     /locus_tag="PLV68_20880"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HRE03763.1"
                     /translation="PGLRHRLRSASTAGWELMVIAYTTVLGLLIGSFLTVVVDRVPDG
                     RSIVAPGSACGNCGLQLGPKDLVPVFSWLALRGRCRQCRAWIGVEPLVLEIATAALFC
                     AFAVHFGLSWDLAAFCVWGAALIGLSWIDLRTKRLPRQIIHVAALVGVPLLVIAALVR
                     DEPRRIWMAALGAGCAFVFMALVYVVSRGQMGDGDVRLAPLLGAYLGYLNPGIVAVGL
                     FLAFLAGALVGLVLMAVGRAGRKTAVPFGPFMAAGAVAAVWAGQPLIDLWIHR"
BASE COUNT          324 a          573 c          524 g          241 t
ORIGIN      
        1 cacccggagg ccttcccgcc gtactacatg ggcatcctcg aatcggccga gctcaccggc
       61 cgcctcgacg agacgttgga gagcctggcc gactacctgg aacgcgagct cgacactcgt
      121 tccaaggtcg tgtcggcgct gtcgtatccc ggcgtggtca tggtgatggc gctgttcacc
      181 gtggccgtgc tggccgggta cgtgctgccc cagttcaagc cgttgttcga agagatgaac
      241 gccgacctgc cacttgccac caggtcgctg ctgttcttcg ccgacgtctt caccatctac
      301 tggtacatcc cgctgaccgg cttcatctgc atgttcgccg gggtgctgtg gctgaccaag
      361 accgagaagg gcaagcgcgt caaagacaag ctggtgctga agatcccggt ggttggcggc
      421 atcatcgact acgccatcct cgaacggttc tgccgcatct tgtcgaccat gatccgcgcc
      481 ggtgttccgc tgccggtcgg gctgcggacc accaccgaag ccacgtcgaa cacgatctat
      541 cgcgagcggc tggagatcgc ccaggccgag atgctggagg gcggcgggtt cagcaagccg
      601 ttggtggaga caggcctgtt ccccggtgcg gccaagcaga tgttcaaggt cggtgaggag
      661 acgggcacgc tcgacgagca gctcgccgtc gcctccacct acttcgaccg cgagttggag
      721 agccggatca agaagttcac cacgatgttc gaaccgatca tgatcgtcgg cgtcggcgtc
      781 atggtcggct tcgtcgcggt cgcgctcgtg tcggcgatgt nnnnnnnnnn nnnnnnnnnn
      841 nnnnnnnnnn nnnnnnnnnn nnnnnnngag cgaaacggga cgggtcaggc gatcagcgat
      901 gaatccacag gtcgatcaac ggttggccgg cccacacggc ggccacggca ccggcggcca
      961 tgaacgggcc gaacggcacc gcggtcttgc gtccggcgcg cccgaccgcc atcagcacca
     1021 gccccaccaa cgcaccggcc aagaacgcca ggaacagtcc gacggcgacg atgcccgggt
     1081 tgaggtagcc gagataggcg ccgagcaacg gggccaggcg cacgtcaccg tcacccatct
     1141 ggccgcggct gacgacgtac accaacgcca tgaacacgaa cgcgcaacca gcgccgagcg
     1201 cggccatcca gatgcgccgt ggctcgtcgc gcaccaacgc ggcgatcacc agcagtggca
     1261 caccgaccaa cgcggcgacg tggatgatct ggcgcggcag gcgcttggtg cgcaggtcga
     1321 tccagctcaa cccgatcaac gctgcgcccc acacgcagaa cgcggcgaga tcccaactca
     1381 acccgaagtg cacggcgaat gcacagaaca gcgccgccgt cgcgatctcc aacaccaacg
     1441 gctcgacgcc gatccaggcc cggcactgac ggcagcgacc gcgcagcgcg agccaactga
     1501 agaccggcac caggtccttg gggccgagtt ggagcccgca gttcccgcac gccgaacccg
     1561 gcgccacgat cgaacgcccg tcaggaacgc gatcgaccac cacggtcagg aacgatccga
     1621 tcagcagtcc caagaccgtc gtatatgcaa tgaccatgag ctcccaaccc gctgtggacg
     1681 cgctccgcag ccggtggcgc agacccgga
//
LOCUS       DAORNF010000753         1709 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_50413_length_1709_cov_1.726723, whole genome shotgun sequence.
ACCESSION   DAORNF010000753 DAORNF010000000
VERSION     DAORNF010000753.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50413_length_1709_cov_1.726723"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="PLV68_20885"
     CDS             <1..402
                     /locus_tag="PLV68_20885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRE03764.1"
                     /translation="ELELAQQLHPKPEHLPLLADSYRALRRYKDVDRIWQTIRESSPA
                     PSVMAEGRIVAAGALADRGDINGALATMMRAADVPKRVREHHLKQWYVIADLYDRSGD
                     IPRARNFFKRIAAIDPRYADVTDRLAALGRR"
     gene            540..872
                     /locus_tag="PLV68_20890"
     CDS             540..872
                     /locus_tag="PLV68_20890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03765.1"
                     /translation="MNLVILAGHLSSNPIERELPSGTRVHNFELTTQVEGAAVTVPLA
                     WIDPRHVPALSVGDEIRVVGSVRRRFFRAGGATQSRTEVVVTSLVRGRDRRGLASQAT
                     LIERLMGA"
     gene            951..>1383
                     /locus_tag="PLV68_20895"
     CDS             951..>1383
                     /locus_tag="PLV68_20895"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798329.1"
                     /note="catalyzes the formation of glycerone phosphate and
                     D-glyceraldehyde 3-phosphate from D-fructose
                     1,6-bisphosphate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HRE03766.1"
                     /translation="MDLHALLGNEADSLLAHTCAGITKDQIHLPGPDFIERVTAVSDR
                     SPQVLRNLGALYNSGRLAGSGYVSILPVDQGIEHSGAASFAPNPAYFDPSNIVELAIE
                     GGCNAVATTYGVLGAVSRKYAHRIPLIVKINHNELMTYPNKF"
     assembly_gap    1384..1422
                     /estimated_length=39
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1423..>1709
                     /locus_tag="PLV68_20900"
     CDS             <1423..>1709
                     /locus_tag="PLV68_20900"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376843.1"
                     /note="catalyzes the formation of glycerone phosphate and
                     D-glyceraldehyde 3-phosphate from D-fructose
                     1,6-bisphosphate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HRE03767.1"
                     /translation="AYDLSAAGVGATIYFGSDQSTRQIQEVAVAFKEAHDLGMFTVLW
                     CYARNNAFKQDGVDYHVSADLTGQANHIGVTIEADIIKQKLPENNGGFEAL"
BASE COUNT          279 a          577 c          529 g          285 t
ORIGIN      
        1 gagttggagc tcgcccagca gttgcacccc aagccggaac atcttccgtt gctggccgat
       61 agctaccgcg cgctgcgccg ctacaaggac gtcgatcgga tctggcagac catccgtgag
      121 tcgtccccgg ctcccagcgt gatggccgag ggtcgcatcg tcgccgctgg cgcgttggcc
      181 gaccggggtg acatcaacgg cgcgctggcc acgatgatgc gtgccgccga tgtgcccaag
      241 cgggtgcgcg aacaccacct caagcagtgg tacgtcatcg ccgatctgta cgaccgttcg
      301 ggcgacatac cccgggcgcg caacttcttc aagcggatcg cggcgatcga tccgcggtac
      361 gcggacgtca ccgatcgtct cgccgcgctg ggccggcgtt gacgtcgacg ttgacgtcgg
      421 cgttgacctc tccgtcgacg gcgctgctga tctgtgcgac accctctgtg tagggtgagc
      481 acaaccgtct tccccctcca cgcccggcgc tgacgcgctg catcccggag gtcggtccca
      541 tgaacctcgt catcttggct ggacacctgt cgtccaaccc catcgagcgc gagttgccct
      601 ctggcacacg cgtccacaac ttcgagctca ccacccaggt cgagggcgcg gcggtgacgg
      661 tgccgttggc gtggatcgat ccacgccatg ttccggcgct gtccgttggt gacgagatcc
      721 gcgtggtcgg ctccgttagg cgtcgcttct ttcgtgcggg cggcgccacc cagagccgca
      781 ccgaggtcgt cgtgacgtcg ttggtccggg gtcgtgaccg ccgtggtctg gcgtcacagg
      841 caacgctgat cgagcgactc atgggagcct gagagtggtg ggcgactacg ctcgcgatca
      901 gcccgcggca tccggctgcc gggcgcgaac cgttcaggag aaccgtcacc gtggacctcc
      961 acgccctgct cggcaacgaa gccgactcgc tgctcgcgca cacgtgcgcc ggcatcacca
     1021 aggatcagat ccacctgccc ggccccgact tcatcgagcg ggtcacggcc gtcagcgatc
     1081 gctcgccgca ggtgctgcgc aacctgggtg cgctctacaa cagcggccgt ctggccgggt
     1141 cgggttatgt ctcgatcctg cccgtcgacc agggcatcga gcactcggga gcggcgtcgt
     1201 tcgcccccaa cccggcctac ttcgacccga gcaacatcgt cgagctcgcg atcgaaggtg
     1261 ggtgtaacgc cgtggcgacc acctatggcg tcttgggggc ggtcagccgc aagtacgccc
     1321 accgcatccc gctgatcgtc aagatcaacc acaacgagtt gatgacctac cccaacaagt
     1381 tcgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnaggcctac gacctgagcg
     1441 cagccggggt cggggcgacg atctacttcg gttcggatca gtcgacccgc cagatccaag
     1501 aggttgccgt ggccttcaag gaggcgcatg acctcggcat gttcaccgtg ctgtggtgct
     1561 acgcccgcaa caacgcgttc aaacaggacg gcgtcgacta ccacgtctcg gccgacctga
     1621 ccggccaggc caaccacatc ggggtgacga tcgaggccga catcatcaag cagaagctgc
     1681 ccgagaacaa cggcggcttc gaggctctg
//
LOCUS       DAORNF010000754         1708 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_50443_length_1708_cov_2.467635, whole genome shotgun sequence.
ACCESSION   DAORNF010000754 DAORNF010000000
VERSION     DAORNF010000754.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50443_length_1708_cov_2.467635"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1427
                     /locus_tag="PLV68_20905"
     CDS             <1..>1427
                     /locus_tag="PLV68_20905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03768.1"
                     /translation="DDGSTLYIDPQGAPPAGPTPGYILDPQRSDRSLASLWSREWTLY
                     YDNAERGTFHLWSEPDASFDVGRWLALTVTPLQGASTTLDGNRRVMVGAHPALVTPIA
                     PGVVSVETWLDAASDGEGGVTTPLSVTAWASGIDLDAIAAVLADLLVVTRPDGAVDIA
                     FAHAADVLPGLVPRQIEQAACCKLTGTDPLPGFRRVLVYRDDGEPTITVETTDHADDA
                     LAQLAGPLLTLRQADRFGVSAVGDRTIRWATTASHLPAESPLRFPWDWDGEFIDGISV
                     AQWHDGSSLVTVAVAGDLAALLATVGDVTRASRAQWEETNMDAYVEQQRQERNALFDD
                     PAETDEADAGFWGTPTVGGRLEVGATEWTLEVWRPDGKAGILRPATGGMTSDFGVSRW
                     WTDLDADDWVHASFSASEVAIVVRVEHMPSAAAIRVTVPGREPVLRPLTVLDSGVALA
                     FDVYTEIGQPTVELLDTAGRVFRGWT"
     assembly_gap    1428..1455
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1501..>1708
                     /locus_tag="PLV68_20910"
     CDS             1501..>1708
                     /locus_tag="PLV68_20910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03769.1"
                     /translation="MNSDDPTNGQPKDVGVGPIAGGNGSLHIGALGTMADADQLARDA
                     EWLDATTRAHAGSALAAGSTRDDAS"
BASE COUNT          273 a          581 c          570 g          256 t
ORIGIN      
        1 gacgatggca gcaccctata catcgacccg cagggcgcac cgccggccgg accaacgcct
       61 ggctatatcc tcgacccaca gcggtccgac cgatcgctgg catcgctgtg gtcccgcgag
      121 tggacgctct actacgacaa cgcagaacgt ggcacgttcc atctgtggag cgaacccgat
      181 gcgagcttcg acgtcggcag gtggctggca ctgacggtca caccgttgca gggcgccagc
      241 accaccctcg acgggaatcg ccgggtcatg gtcggggcgc atccagcact cgtcaccccc
      301 atcgctccag gcgttgtcag cgtcgagacc tggctcgacg ctgcctccga tggtgagggg
      361 ggcgtcacca ccccgctgag cgtcaccgcg tgggccagcg ggatcgacct cgacgcgatc
      421 gcggcggtgc tcgctgatct cctcgtcgtc acgcgaccgg atggtgccgt cgacatcgcg
      481 ttcgctcacg ccgccgacgt gctccccgga ctcgtaccgc gccagatcga gcaggccgcc
      541 tgctgcaagc tgacaggcac tgacccgctc ccagggttcc ggcgggtact ggtgtatcgg
      601 gacgacggtg aaccgacgat caccgtcgag accaccgatc acgccgacga cgccttggct
      661 caacttgccg gaccgctcct gacgctgcgg caagcggacc ggttcggtgt ctcggccgtg
      721 ggcgacagaa cgattcgctg ggcgaccact gcctcgcatc tccccgccga atcgccgctg
      781 cgctttccgt gggattggga cggcgaattc atcgacggga tcagcgtcgc gcagtggcac
      841 gacggttcct ccctcgtcac ggtcgcagta gctggcgacc ttgccgcatt actcgccacg
      901 gtgggcgacg tcacacgggc gtcgcgcgcc cagtgggagg agaccaacat ggatgcgtac
      961 gtcgagcagc agcgccagga gcgcaacgcg ctgttcgacg accctgccga gacggacgag
     1021 gccgatgcag gcttctgggg cacaccgacc gtcggcggac ggttggaggt cggtgcaacg
     1081 gagtggacgc tggaggtgtg gcgacccgat ggcaaggcgg gcatcctccg gccagcgacg
     1141 ggcggcatga cgtcggactt cggtgtcagc cggtggtgga ccgatctcga tgccgacgac
     1201 tgggtccacg ccagcttttc agccagcgag gtcgcaatcg tcgtgcgggt cgaacacatg
     1261 ccctcggcgg ccgcgatccg ggtcaccgtg cccggacgcg agcccgtcct gcgcccgctg
     1321 accgtgctgg acagcggggt cgcgctcgcg ttcgacgtct acacggagat cggccagccg
     1381 accgtcgaac tgctcgacac cgccggtcgt gtgttccgcg ggtggacnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnccccg gccatacctt cgagatcgtt gcagggacag gttcaggccg
     1501 atgaactccg acgacccgac caacgggcaa cccaaggacg tcggggtcgg cccgatcgcc
     1561 gggggtaacg ggtcgcttca catcggtgcg ctggggacca tggccgatgc cgaccagttg
     1621 gcacgcgacg cggaatggct ggacgcgacc actcgcgcgc acgcgggctc cgcgctcgcc
     1681 gctggcagca ctcgtgacga tgccagca
//
LOCUS       DAORNF010000755         1708 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_50462_length_1708_cov_1.308530, whole genome shotgun sequence.
ACCESSION   DAORNF010000755 DAORNF010000000
VERSION     DAORNF010000755.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50462_length_1708_cov_1.308530"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="PLV68_20915"
     CDS             complement(<1..238)
                     /locus_tag="PLV68_20915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03770.1"
                     /translation="MRVPEDLLYSSDHEWVRVGDGVVRIGITDYAQDALGDVVFVQVP
                     DQGATVAAGQNLVGSVTQNGHREIGVILGSQDRYS"
     gene            complement(259..>691)
                     /locus_tag="PLV68_20920"
     CDS             complement(259..>691)
                     /locus_tag="PLV68_20920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03771.1"
                     /translation="QQRGEFGFGKMRQGLVQAVAGGGHFAQFQLHHGSGDDDPGVMRI
                     FAKQAREALFGGTVFVATIFGMKRFDVTWWGKLATFLLMFAIPGLLIGASDLGAARGF
                     NIAAWILAIPGLIISYATAVRYIPVIRTNLREGRAERNAAH"
     assembly_gap    692..704
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<705..891)
                     /locus_tag="PLV68_20925"
     CDS             complement(<705..891)
                     /locus_tag="PLV68_20925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03772.1"
                     /translation="MTSELGVQGEEAAGRPSYTDNIWTVPNLMSLIRLLCIPLFLYLL
                     FGRDNRAGAAWLLGGLGA"
     assembly_gap    920..973
                     /estimated_length=54
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<974..1343)
                     /locus_tag="PLV68_20930"
     CDS             complement(<974..1343)
                     /locus_tag="PLV68_20930"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HRE03773.1"
                     /translation="MSTVFTKIIEGQIPGTFVWRDDRCVAFMSVNPMAVGHTLVVPIA
                     AVDHWLDLDADLTAHLFDVSRRIGLAQQAAFGCTRVGLIIAGYEVPHTHIHVIPTDDM
                     DQLSFANAAASVDRDTLESAA"
     assembly_gap    1423..1451
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1452..>1708)
                     /locus_tag="PLV68_20935"
     CDS             complement(<1452..>1708)
                     /locus_tag="PLV68_20935"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HRE03774.1"
                     /translation="RSDDPFCCHKTTFRRHYDEARARHPDADDVVLVNEHGCVVETTI
                     ATIAARVGGRWYVPPLGDGGLDGIGRAVALAAGQVEERSFTV"
BASE COUNT          288 a          544 c          544 g          236 t
ORIGIN      
        1 cgctgtagcg gtcctggctg cccaggatca cgccgatctc gcggtggcca ttctgggtga
       61 cggagccgac caggttctgg ccggcggcca cggtggcgcc ctggtccggg acctgcacga
      121 acacgacgtc gccgagggcg tcctgggcgt agtcggtgat accgatgcgc acaacaccgt
      181 cgccgacacg aacccactcg tgatcgctcg aatacaagag gtcttccggc acgcgcatgg
      241 cgtaacggta gcaagtgttc agtgggcagc gttgcgctcg gcgcggccct cacgcaggtt
      301 ggtgcggatc acggggatgt agcggaccgc tgtggcatag ctgatgatca gaccggggat
      361 ggccaggatc caggccgcga tgttgaaccc gcgcgccgcg cccaggtcgg acgcgccgat
      421 cagcagcccc gggatcgcga acatcagcag gaacgtcgcc agcttgcccc accaggtgac
      481 atcgaagcgc ttcatgccga agatggtggc gacgaacacc gtgccgccga acaacgcctc
      541 acgcgcctgc ttcgcaaata tccgcatcac gcctgggtcg tcgtcacccg agccatggtg
      601 caactggaac tgggcgaaat ggccgccgcc cgcgacggcc tgtacgaggc cttgccgcat
      661 cttgccgaag ccgaactcgc cacgctgctg tnnnnnnnnn nnnntcgcac ccaacccgcc
      721 gagcaaccac gcggcccccg cccggttgtc gcggccgaac agcaggtaga ggaacagggg
      781 gatgcacaac aaccggatca acgacatcag gttgggcacc gtccagatgt tgtcggtata
      841 cgacggccgc ccggccgcct cctccccctg cacacccaac tcagacgtca cagcagcaca
      901 gtagtgctcg catcgctgcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      961 nnnnnnnnnn nnncggcggc ggactccagg gtgtcgcggt cgaccgaggc tgcggcgttg
     1021 gcgaaggaca actggtccat gtcgtcggtg ggaatgacgt ggatgtgggt gtgcgggacc
     1081 tcgtagccag cgatgatcag cccgacccgg gtgcagccga aggcggcttg ctgggccagg
     1141 ccgatgcggc ggctgacgtc gaacaggtgc gccgtcaggt cggcgtcgag gtcgagccag
     1201 tggtcgaccg ccgcgatcgg caccaccagc gtgtgcccga ccgccatcgg gttgaccgac
     1261 atgaaagcca cgcagcggtc gtcgcgccac acgaacgtgc cgggaatctg cccctcgatg
     1321 atcttggtga agaccgtgct catgcgcccg atcgtagaac cctcgccagc cagcacccgc
     1381 gttcagagca cgtgggcgcg gcgccaacca cgcagcgcac tgnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn naccgtgaac gagcgctctt cgacctggcc agcagcgagc gcgaccgcgc
     1501 gtccgatgcc gtcgagaccg ccatcgccga gcggcggtac gtaccagcgc ccacccacgc
     1561 gcgccgcgat ggtggcgatc gtcgtctcca cgacgcagcc gtgttcgttg accagcacca
     1621 catcgtcggc gtcgggatga cgggcgcggg cctcgtcgta gtgacggcgg aaggtggtct
     1681 tgtggcagca gaaggggtcg tcggagcg
//
LOCUS       DAORNF010000756         1707 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_50488_length_1707_cov_2.059322, whole genome shotgun sequence.
ACCESSION   DAORNF010000756 DAORNF010000000
VERSION     DAORNF010000756.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50488_length_1707_cov_2.059322"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(230..988)
                     /locus_tag="PLV68_20940"
     CDS             complement(230..988)
                     /locus_tag="PLV68_20940"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HRE03775.1"
                     /translation="MTSRDGFGSEWARDRDDLDHLGGAFARVFDFLALRHEITARDAI
                     FCFGSRDPDVPAVAAALFHDGAAPWVVTTGGVELDDGRCEAAVFADDLAALGVPTDRI
                     IVEGYSTDTSDNVVLGVAALAAQIGPVRSLLSVAWPFAARRCVATLAVHHPEIAVCSV
                     PATPDPATRSPLNPATASWANAQLATLIRYGERGLIVPQAVPADVIAAGKAIAATLAA
                     GAHRPGQPEWLPRQQRLDRAQHVRIDQQSAPAAR"
     gene            1099..>1707
                     /locus_tag="PLV68_20945"
     CDS             1099..>1707
                     /locus_tag="PLV68_20945"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HRE03776.1"
                     /translation="MTEPSDDPILYEVADHVATITLNRPHRSNAISMEMLSQLADRLG
                     EADLDKDVRAVILTGAGKAFCAGLDIKDAVAGTGIGGNSSGSGQGAHYNTRNHPTTVM
                     FEMDTPVIGAINGAAAGFGLDIALGCDIRIVAAEAKIATGFAKRGVVPESGGTWYLPR
                     LLGWAKAAEIGFLGRTLFQELSKLGGPPGWALSAAIASSTTVV"
BASE COUNT          270 a          580 c          609 g          248 t
ORIGIN      
        1 ggaggaagtc gcgcggttgg cagcgtgctg gccggtttcg agaaccacca cggccgcacc
       61 gagttgggca gtcgcagccg accgctcggc cgggtggtgg cggggttcgg caacaacggc
      121 cgcgatcaca ccgaaggtgc cgtgacggga acggtgatcg gcacctacct gcacggtccg
      181 ctgttggcca agaacgtgtg gttggccgat gcgctgatcg cggcggcgct caacgcgccg
      241 ctggagccga ttgctgatcg attcgaacgt gctgtgcacg atcgagccgt tgctgtcgcg
      301 ggagccactc cggctgacct gggcggtgcg ccccagcggc caaggtggcg gcgatcgcct
      361 taccggcggc gatgacgtcc gccggtacgg cttggggcac gatgaggccg cgttcgccgt
      421 agcggatcag ggtggcgagt tgggcattgg cccacgacgc ggtggccggg ttgagcggtg
      481 agcgcgtggc cggatcgggc gtggccggta ccgagcacac cgcgatctcg ggatggtgca
      541 cggccaacgt ggcgacgcag cgccgggccg cgaagggcca cgcgaccgag agcagcgagc
      601 gcaccggtcc gatctgggcc gccagcgcgg ccacaccgag cacgacgttg tcgctcgtgt
      661 cggtggagta accctcgacg atgatgcggt cggtcggcac gccgagggcc gccaggtcgt
      721 cggcgaacac agcggcctcg cagcggccgt cgtccaactc gaccccgccc gtcgtcacca
      781 cccatggggc ggcaccatcg tggaacagcg ccgcggcgac ggcgggcaca tccgggtcgc
      841 ggctgccgaa gcagaagatc gcgtcgcgag cagtgatctc gtgacgcagc gcgaggaagt
      901 cgaacacgcg ggcgaaggca ccaccgaggt gatcgagatc gtcgcggtcg cgcgcccatt
      961 cacttccgaa cccgtcccga ctcgtcactc gctgcacaat agtccccgcc ccgacgccgc
     1021 gttcccctcg ccgtcagtca cgcctgcgcc gtggcgcccg atgcgccgcc gccgtccgat
     1081 gtggggcagc atctgggcat gaccgagccc agcgatgacc cgatcctgta cgaggtggcc
     1141 gaccacgtcg ccacgatcac gctcaaccgc ccgcaccgca gcaacgcgat ctcgatggag
     1201 atgctgtctc agttggccga tcggctcggc gaggccgacc tcgacaagga cgtgcgcgcg
     1261 gtgatcttga ccggcgcggg caaggcgttc tgtgccgggc tcgacatcaa ggacgccgtt
     1321 gccggcaccg gcatcggcgg caactcgtca gggtcgggcc agggtgcgca ctacaacacc
     1381 cgcaaccacc cgaccacggt gatgttcgag atggacacgc cggtgatcgg cgcgatcaac
     1441 ggcgccgcag ccgggttcgg tctcgacatc gcgctcgggt gcgacattcg catcgtcgcc
     1501 gccgaagcca agattgccac gggcttcgcc aagcgcggtg tggtgcccga gagcggcggc
     1561 acctggtacc taccccgcct gctcggctgg gccaaggcgg ccgagatcgg cttcctcggg
     1621 cgtacgctct ttcaagaatt gagcaaactg ggtgggccgc ccggctgggc gctttcggcg
     1681 gccattgcct cttccaccac ggtagtg
//
LOCUS       DAORNF010000757         1706 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_50532_length_1706_cov_1.849788, whole genome shotgun sequence.
ACCESSION   DAORNF010000757 DAORNF010000000
VERSION     DAORNF010000757.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50532_length_1706_cov_1.849788"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /gene="ruvX"
                     /locus_tag="PLV68_20950"
     CDS             <1..204
                     /gene="ruvX"
                     /locus_tag="PLV68_20950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HRE03777.1"
                     /translation="AQIEIDQIATVVGVPVDTIDERLTTVTANRMLADRGVRGPERRK
                     VIDKIAAAVILQTWLDGRPESSC"
     gene            213..>850
                     /locus_tag="PLV68_20955"
     CDS             213..>850
                     /locus_tag="PLV68_20955"
                     /inference="COORDINATES: protein motif:HMM:NF014656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HRE03778.1"
                     /translation="MARRADPLLDPLDDADFWVDDEWDDIEDGQVAVVERVGRTHRIA
                     KWLVVTTSIVLVAALLVGGGVGWWYIRQVNPSGEATERINFTVADDDTLVTISHRLAD
                     LGVITDAGVFQYYVTSNGGLTITPGYYQLLPRDHMGNLMRVLNTPPAATYKRVTFPEG
                     FTLIQMGRRVERDLPPMTAERFAAAAASGQVQSPYLPPGSTNLEGLLFPDTY"
     assembly_gap    851..860
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <861..1443
                     /gene="mltG"
                     /locus_tag="PLV68_20960"
     CDS             <861..1443
                     /gene="mltG"
                     /locus_tag="PLV68_20960"
                     /inference="COORDINATES: protein motif:HMM:NF014656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HRE03779.1"
                     /translation="GSGSPSSGPGAARRARRPWARAVRSHERVGRQVGLEAKAAEMGL
                     TPYQVLIIASMVEREAKFDEDRPKIARVIFNRLFLQMELQIDATLYYGQDPSTPFSQL
                     KRTPGPYNSYLNKGLPPTPIANPGRASIEAVLNAAPNPSQGDPICRAITDGSPCIYLY
                     YVIADEEGHHVFAATLSQHNANVRAAREKGLLS"
     gene            1440..>1706
                     /locus_tag="PLV68_20965"
     CDS             1440..>1706
                     /locus_tag="PLV68_20965"
                     /inference="COORDINATES: protein motif:HMM:NF020090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03780.1"
                     /translation="MITAATRVAGVIGSPVYHSLSPVIHNAAFAANGLDWVYAAFEVA
                     PGRAGAAIDAMRTLGLAGLSVTMPHKDDVARAVDVLDDASRVLGT"
BASE COUNT          276 a          586 c          577 g          257 t
ORIGIN      
        1 gcccagatcg agatcgacca gatcgctacc gtggtgggcg tgccggtcga cacgattgac
       61 gaacgcctca cgacggtgac ggccaaccgc atgttggccg atcgcggcgt gcgcggtccc
      121 gagcggcgca aggtcattga caagatcgcc gcggccgtca tcctgcagac ctggctcgac
      181 ggccggcccg agagcagctg ctgaggcgca ccgtggcccg ccgcgccgat ccgttgctgg
      241 atcccctcga cgacgccgac ttctgggtcg acgacgagtg ggacgacatc gaagacggcc
      301 aggtcgcggt cgtcgagcga gtcgggcgca cgcatcgcat cgccaagtgg ttggtcgtga
      361 cgacgtcgat cgtgctcgtc gccgcgctgt tggtgggcgg cggtgtcggg tggtggtaca
      421 tccgccaggt caaccccagc ggcgaagcga ccgagcggat caacttcacc gttgccgacg
      481 acgacacgct ggtcacgatc agccaccggt tggctgacct cggggtgatc accgacgccg
      541 gcgtgtttca gtactacgtc accagcaacg gtgggctcac catcacgcct ggctattacc
      601 agctgctgcc gcgcgaccac atgggcaacc tgatgcgggt gctcaacacg ccgcccgcgg
      661 cgacgtacaa gcgggtgacg tttcccgaag ggttcacgct gatccagatg gggcgccggg
      721 tcgagcggga cctgccgccg atgaccgccg agcggttcgc cgcggccgcg gcgtcgggtc
      781 aggtgcagtc gccgtacttg ccgccgggct cgaccaacct ggaagggttg ctgttccccg
      841 acacctatca nnnnnnnnnn ggggtctggc tcgccgtcga gcggccctgg cgccgcgcgc
      901 cgcgcgagaa ggccgtgggc ccgggcagtc aggagccatg agcgggtcgg gcgtcaggtc
      961 ggcctggagg ccaaggcggc agagatgggg ctcacgccgt atcaggtgct gatcatcgcg
     1021 tcgatggtcg agcgggaggc gaagttcgac gaggaccgcc cgaagatcgc ccgtgtgatc
     1081 ttcaaccggc tgttcttgca gatggaacta cagatcgacg ccacgttgta ctacggccag
     1141 gatccctcga cgccgttcag ccaactgaag cgcacccccg gcccgtacaa cagctacctc
     1201 aacaaggggc tgccgccgac gccgatcgcc aaccccggcc gagcctcgat cgaagcggtg
     1261 ctcaacgccg cgcccaaccc gtcgcagggt gacccgatct gccgggcgat caccgacggc
     1321 tccccgtgca tctacctgta ctacgtgatc gccgacgagg agggccatca cgtgttcgcg
     1381 gccacccttt cccagcacaa cgccaacgtg cgcgccgctc gcgagaaggg actgctgtcg
     1441 tgatcaccgc ggccacccgg gttgccgggg tgatcggttc gccggtgtac cacagcctca
     1501 gcccggtcat ccacaacgcg gcctttgccg ccaacgggct cgactgggtg tacgccgcgt
     1561 tcgaggtcgc gcccggccga gccggtgccg cgatcgacgc gatgcgcacg ctcggcctcg
     1621 cgggcctgtc ggtcaccatg ccgcacaagg acgacgtcgc ccgggcggtc gacgtactcg
     1681 acgacgcgtc gcgggtgctc ggcacc
//
LOCUS       DAORNF010000758         1705 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_50569_length_1705_cov_1.212727, whole genome shotgun sequence.
ACCESSION   DAORNF010000758 DAORNF010000000
VERSION     DAORNF010000758.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50569_length_1705_cov_1.212727"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>428)
                     /locus_tag="PLV68_20970"
     CDS             complement(<1..>428)
                     /locus_tag="PLV68_20970"
                     /inference="COORDINATES: protein motif:HMM:NF014640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HRE03781.1"
                     /translation="VRVQGEFAAEMVSSAIRQLGRRDDIDVVVVIRGGGARTELAVFD
                     DESIAVAIATSVVPVFTGLGHEIDRSIADEVAHTAFKTPTACAAHLVAAVDEYAAIAD
                     NAWKAIEVRVARHLERANQGLVDRGVAARGHADRPWRAPSA"
     assembly_gap    429..556
                     /estimated_length=128
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<557..>814)
                     /locus_tag="PLV68_20975"
     CDS             complement(<557..>814)
                     /locus_tag="PLV68_20975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03782.1"
                     /translation="LDVSFFANVRMRIRPLLHKHRLRLADGIKVRIFGGLDFYAPSGR
                     LSLKMQGIDPRYTLGEMAVQRDEIVRRLIAGGLYDANRQRRV"
     assembly_gap    815..896
                     /estimated_length=82
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1286..1435
                     /estimated_length=150
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1436..1682)
                     /locus_tag="PLV68_20980"
     CDS             complement(<1436..1682)
                     /locus_tag="PLV68_20980"
                     /inference="COORDINATES: protein motif:HMM:NF012401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRE03783.1"
                     /translation="MNDEVLPVRNGFPARIVVPGLYGYVSAVKWLSEIRLTRFEDEQG
                     YWIPRGWAALGPVKTQSRIDRPRRTIKAGHTVIGGIAG"
BASE COUNT          199 a          480 c          462 g          204 t
ORIGIN      
        1 gcggatggcg cgcgccaggg acgatctgcg tgcccgcgcg ccgcgacacc gcgatcgacc
       61 aggccctggt tggcccgttc gaggtggcgc gccacccgaa cttcgatcgc cttccaggcg
      121 ttgtctgcga tcgcggcgta ctcgtcgacc gcagcgacga gatgagccgc gcacgcggtg
      181 ggcgtcttga acgcggtgtg ggcgacctcg tcggcgatgc tgcggtcgat ctcgtggccc
      241 agtccggtga acacgggtac caccgatgtg gcgatcgcga ccgcgatcga ctcgtcgtcg
      301 aacaccgcaa gctcggtgcg cgcgccaccg ccgcggatca ccaccacgac gtcgatgtcg
      361 tcgcggcggc cgagttggcg gatggccgac gacaccatct ccgcggcgaa ctcaccctgc
      421 acgcgcacnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      541 nnnnnnnnnn nnnnnnaccc gccgctggcg gttggcgtcg tacagcccac cggcgatcag
      601 acggcggacg atctcgtcgc gctgcaccgc catctcaccc agggtgtagc gggggtcgat
      661 gccctgcatc ttcaggctga gccgcccgct gggggcgtag aagtcgagcc cgccgaagat
      721 acgcaccttg atgccgtcgg ccagacggag gcggtgcttg tgcagcaatg ggcggatgcg
      781 catgcggacg ttggcgaaga acgacacgtc gagcnnnnnn nnnnnnnnnn nnnnnnnnnn
      841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnccgt
      901 agccctggat ctcgcctcgc acccacacgc cgtcgccgaa ctggcgccgc aacgacccgt
      961 tgatcgcgtc ggccagctca cccaccgaat aggtgggctc attcccgtcg tcgagatcga
     1021 agtcgaagct ggcctgactc acgccgccga gtctggcacg ggggtgtcac gaggcggtgg
     1081 gtgcacgggg ctgtgaagcg ggccgtggcc tattcgtcca gcgcacggca ccgacccgcg
     1141 ccgctgctgg tgacccagcg cggcagtcac gccgtcacgt cgtcacgtca ctgtgatcgt
     1201 gatcgtgtgc cagccggtgg cgccgtcggg gtcgacccgg gcgcgctgtt cggtctgggt
     1261 ctcaccgcgg ccgtcggtgg cgcgcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncgccg
     1441 gcgatgccgc cgatcaccgt gtgcccggcc ttgatcgtgc gccgcggccg gtcgatgcgc
     1501 gactgggtct tgaccgggcc gagcgcggcc cacccgcggg gaatccaata cccctgctcg
     1561 tcctcgaacc gggtgaggcg aatctcgctc agccacttca ccgcggacac gtagccgtac
     1621 agacccggga ccacgatccg cgccgggaag ccgttgcgca ccggcagcac ctcgtcgttc
     1681 atgccgaaca cgaccatcgc gtcgc
//
LOCUS       DAORNF010000759         1704 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_50595_length_1704_cov_1.811401, whole genome shotgun sequence.
ACCESSION   DAORNF010000759 DAORNF010000000
VERSION     DAORNF010000759.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50595_length_1704_cov_1.811401"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            128..1138
                     /locus_tag="PLV68_20985"
     CDS             128..1138
                     /locus_tag="PLV68_20985"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRE03784.1"
                     /translation="MTDSITQHDSMLAALPLGVEVRHLAALEAIAATSSFSQAAQLLG
                     YAQSAVSQQIATLERAVGHKLVERPGGPRPVSLTAAGEVLLLHATHITARLGAAKADL
                     DALAAGNIGSLRVGTFQSASARLLPPTLNRFRQEFPHVTVELRNEQSAVELDELVRAG
                     QLDLAFTEGDTHLPPLASAPMIADPYVVIVAPDNPIAAASSIRLVDLHEAHIIAGSAM
                     SSCESIILGAVERAGAKPLTVFRSDDNLTTQRLVAAGLGVAIAPLLAIELLVADAAVA
                     VIPVADAELSTRSISIVWHQDRYRSRAAQAFVDTAAEVAANIAMPNIPLVTSAPAPSL
                     YV"
     gene            complement(1175..>1704)
                     /locus_tag="PLV68_20990"
     CDS             complement(1175..>1704)
                     /locus_tag="PLV68_20990"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HRE03785.1"
                     /translation="IAVAVLSGRGPIVVVEPSPTRREMATHLGAHAVHEPGTPLADLL
                     SDIGWRRPTISPLLDADPVVATIFECVGSPVPVQTILAQAPAHSRVVLAGACTAPVEI
                     SPLQLTTTEVTVETTYAYRQADLVAAAAHLQARPDVFERFITREVGLDGAAAAFDALA
                     TDPREVKVLIHPSHA"
BASE COUNT          272 a          605 c          559 g          268 t
ORIGIN      
        1 cgcgggccga gcgcggagat cacgccgaag aaggcgagag tcagcagcag tatcatgttg
       61 atcacctcct gtgattagtc ttgggttcat tatcactccg tctgatccat aacggaaatg
      121 catatttatg accgattcga tcacacaaca cgattcaatg ctcgcggcct tgccgttagg
      181 ggtcgaagta cgtcacctcg cggcgttgga agcgatcgcc gcgacctcgt cgttctccca
      241 ggcagcgcag ctgctggggt acgcccagag cgcggtcagc caacagatcg cgacgctgga
      301 acgagccgtc gggcacaagc tggtcgaacg ccccggtggg ccacgtccgg tgtcgctcac
      361 cgccgcgggc gaagtgctgc tgctgcacgc gacgcacatc accgcccggc tcggcgcggc
      421 caaggccgat ctcgacgcgc tcgcagcggg caacatcggg tcgttgcggg tcggcacctt
      481 ccagtcggcc agcgcccggc tgctgccgcc caccctcaac cgcttccgcc aggagttccc
      541 ccacgtcacg gtcgagctcc gcaacgagca gtcggcggtc gagctcgacg agctggtccg
      601 cgccggccaa ctcgacctgg ccttcaccga gggcgacacc cacctaccgc cgctcgcgtc
      661 cgcgccgatg atcgccgacc cgtacgtggt gatcgtcgcc ccggacaacc cgattgcagc
      721 cgcttccagc attcgtctgg tcgatctgca cgaggcccac atcatcgccg gatcagcgat
      781 gagcagttgc gaatcgatca tcctcggcgc ggtcgaacgg gccggagcca agccgctcac
      841 ggtgttccgc agcgacgaca acctcaccac gcagcggctg gtggcggccg ggctcggcgt
      901 cgccatcgct cccctcctgg cgatcgagtt gctggtggcc gacgcggcgg tggccgtgat
      961 ccccgtcgcc gacgccgaac tgtcgacccg ttcgatctcg atcgtgtggc atcaggatcg
     1021 ctaccgctct cgagccgcgc aagccttcgt cgataccgcg gccgaggtcg cggccaacat
     1081 cgcgatgccg aacatccccc tcgtcacctc cgccccagcg ccatcactct acgtgtaggc
     1141 gctctccgtg taggcgctgt ccgtgtaggc gccatcaggc gtgtgacggg tggatcagca
     1201 ccttgacctc gcgggggtcc gtcgccaacg cgtcgaacgc cgcggctgcc ccgtcgaggc
     1261 cgacctcgcg ggtgatgaac cgttcgaaca cgtcggggcg cgcctggaga tgagcggccg
     1321 cggcgaccag atcggcctgg cggtaggcgt aggtggtctc gaccgtgacc tcggtggtgg
     1381 tcagttggag cgggctgatc tcgaccggcg cggtgcaggc accggccagc accacccggc
     1441 tgtgcgcagg cgcctgggcc aagatcgtct gcaccggcac cgggctgccg acgcactcga
     1501 agatcgtcgc caccacgggg tccgcgtcga gcagcggtga gatggtcggc cgccgccacc
     1561 cgatgtcgct gagcaggtcg gccaacggcg tgccaggttc gtgcaccgca tgagcgccga
     1621 ggtgggtggc catctcgcgc cgggtcggcg acggctcgac caccacgatc gggccacggc
     1681 ccgacagcac cgcgacggcg atga
//
LOCUS       DAORNF010000760         1703 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_50648_length_1703_cov_1.831311, whole genome shotgun sequence.
ACCESSION   DAORNF010000760 DAORNF010000000
VERSION     DAORNF010000760.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1703
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50648_length_1703_cov_1.831311"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="PLV68_20995"
     CDS             <1..255
                     /locus_tag="PLV68_20995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03786.1"
                     /translation="PMPFPNGADYSLWLLPTYVRQEDGHMRGLMPGMLWMPQDRPYSD
                     QTIVDNVVGQAGKKFLLVRTQYSSETEGAQIAFDITGPWR"
     gene            316..>825
                     /locus_tag="PLV68_21000"
     CDS             316..>825
                     /locus_tag="PLV68_21000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03787.1"
                     /translation="MLGSMSASYNGAQQAIDISAPNAQSILRFNETASGDFWFEADVE
                     LLTDPSTRKHMGLWMTTGNGAEGYRFAHLDGAWSVSRWNSGFGDGAAVTGGVNDNANP
                     VAGVSDAAPAFNVGQRMILRCEVIVGAFDANGVPWARLIQFKAGGVLMFQVGDASYRG
                     KLIPGVFLYG"
     assembly_gap    826..858
                     /estimated_length=33
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <859..1342
                     /locus_tag="PLV68_21005"
     CDS             <859..1342
                     /locus_tag="PLV68_21005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03788.1"
                     /translation="PSGLPAFPATVGVNADDELMRLAGGSTSVLPDPAADIGVNAHVD
                     LIRRNSPSSELWSRGGGYDWCLRTIPSARKDIHFSGHGVIAGTVKEKGQPDQPLVRRV
                     QLISENARVLVAETWSDTDGNYRFELLDPAQRYTVVSYDHKQMYRAVIADNLRPEMMP
                     "
     assembly_gap    1376..1420
                     /estimated_length=45
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1472..>1703
                     /locus_tag="PLV68_21010"
     CDS             1472..>1703
                     /locus_tag="PLV68_21010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03789.1"
                     /translation="MLVEIKLTKPAGTIAGGLLTLTQQEDGLITATGIATWARLVNGN
                     EVNALDLDCSGTDGNGDVKLASTNLYLGGDARM"
BASE COUNT          305 a          527 c          544 g          249 t
ORIGIN      
        1 ccgatgccgt tcccgaacgg agcggactac agcctgtggc tgctgccgac ctacgtgcga
       61 caggaggacg gccatatgcg cggcctgatg ccgggaatgc tgtggatgcc tcaagaccgt
      121 ccgtacagcg accagaccat tgtcgacaac gtggtcggac aggcgggcaa gaagttcctg
      181 ctggtcagga cgcagtacag ctcggaaacc gagggggcgc agatcgcgtt cgacatcacc
      241 gggccgtgga ggtgatccgt gagctacccg ctcaacgaca ccttcgccac ggcaccggcg
      301 aacggctaca ccacggtgct cggcagcatg tccgccagct acaacggcgc ccagcaggcc
      361 atcgacatct cggcgccgaa tgcccagtcg atcctgcgtt tcaacgagac ggccagcggt
      421 gacttctggt tcgaagccga tgtcgagctg ctgaccgacc cgagcacccg caagcacatg
      481 ggcctgtgga tgacgacggg caacggagcc gagggctatc ggttcgcaca tctcgatggt
      541 gcgtggagcg tctcccgctg gaacagcggc tttggtgacg gcgcggcggt gacgggcggc
      601 gtcaacgaca acgcaaatcc cgtcgccgga gtgtccgacg cggccccggc cttcaacgtc
      661 ggccagcgga tgatcctgcg ctgcgaagtc atcgtcggcg cgttcgatgc taacggcgtg
      721 ccttgggcac ggctgatcca gttcaaggcc ggcggcgtgc tgatgttcca ggtcggcgat
      781 gcgagttacc ggggcaagct gattccgggc gtgtttctgt acgggnnnnn nnnnnnnnnn
      841 nnnnnnnnnn nnnnnnnncc cgtctggttt gcccgctttt ccggcgacgg tgggcgtgaa
      901 cgccgacgac gagctgatgc ggctggccgg cggctcgacc tcggtgctgc ccgatcccgc
      961 agccgatatc ggcgtcaacg ctcacgtcga tctgatccgc cgcaacagcc catcgagcga
     1021 gttgtggagc cggggtggcg gctacgactg gtgcttgcgc acgattccca gcgcgcgcaa
     1081 ggacatccac ttcagcggcc acggcgtgat cgccgggacg gtcaaggaga agggccagcc
     1141 cgaccaaccg ctggtgcgac gggtgcagct catcagcgag aacgcccgcg tcctggtcgc
     1201 cgagacctgg tctgacaccg acggcaacta ccgattcgaa ctgctggacc cggcgcagcg
     1261 atacaccgtg gtcagctacg accacaagca gatgtaccgc gccgtgatcg cggacaacct
     1321 acgcccggag atgatgccgt gaccgtcgcc atcactgtcg aacacaacga ggcgcnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn atctacggcg ggacacggcc
     1441 cgccacgccg gccacgacac cgaccagtgc gatgcttgtc gagatcaagt tgaccaagcc
     1501 cgcgggcacg attgcgggcg ggctgctcac actgacgcag caggaggacg ggctgatcac
     1561 ggcgaccggc attgcaacct gggcccggct cgtgaatggc aacgaggtga acgccctcga
     1621 tctggactgc agcggcaccg acggcaacgg cgacgtgaaa ctggccagca ccaacctgta
     1681 tctgggcggt gacgcccgga tgg
//
LOCUS       DAORNF010000761         1701 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_50721_length_1701_cov_1.715674, whole genome shotgun sequence.
ACCESSION   DAORNF010000761 DAORNF010000000
VERSION     DAORNF010000761.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50721_length_1701_cov_1.715674"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="PLV68_21015"
     CDS             <1..528
                     /locus_tag="PLV68_21015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03790.1"
                     /translation="PPDTEPPRPPSVADIPPIPEPQVFTGFGAHQVMSDPLPSGLAYG
                     DVATAYQTAKDVADWVAAGSWDTLRSRLPNDATLDDASFEFRYGPIDKMSLMLLDAVL
                     ASNGGGFDFTLGAISNDVASNSTTLWCLDWYADAPGFAVVHRQEVLDQWPQLISVEAF
                     RNNPELVANLSVRCQ"
     assembly_gap    574..642
                     /estimated_length=69
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(694..900)
                     /locus_tag="PLV68_21020"
     CDS             complement(694..900)
                     /locus_tag="PLV68_21020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03791.1"
                     /translation="MRSRPIELVPDGSGNERPTSGPPTTVSVAATSPDDAAAPTVLVN
                     SITSPLRRPASLSTMSAAITRDWW"
     gene            1020..>1327
                     /locus_tag="PLV68_21025"
     CDS             1020..>1327
                     /locus_tag="PLV68_21025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03792.1"
                     /translation="MRSLQRVVLRPGDTVTIGETTLSAVLHRTTGGTRVAGGHSIAFN
                     RSPRLDPRYAGAELIAPDPPQRPGHQRFPLMTVIAPILMGAIMYSVTKNVLSILFVGL
                     S"
     assembly_gap    1328..1375
                     /estimated_length=48
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1402..>1701
                     /locus_tag="PLV68_21030"
     CDS             1402..>1701
                     /locus_tag="PLV68_21030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03793.1"
                     /translation="MTAATAGFRASLRDLAVQAQYAAEQERVGRRAEHPSIEEIVDAT
                     HRLSPLVWTRRPSYRSFATVRLGLGDQPSRNTIETTRTNNTLPELWREMHDVVRQF"
BASE COUNT          261 a          577 c          500 g          246 t
ORIGIN      
        1 ccacccgaca cggaacctcc ccggccgccc agcgtggccg acattccgcc gattcccgaa
       61 ccacaggtgt tcaccggctt cggtgcccac caggtcatgt ccgacccgct gcccagcggg
      121 ctcgcgtacg gcgacgtcgc cacggcctat cagaccgcca aagacgtggc cgactgggtt
      181 gccgcgggtt cgtgggacac gcttcgctcc cgtttaccca acgacgccac gctcgacgac
      241 gcgtcgttcg agttccgcta tgggccgatc gacaagatgt cgttgatgtt gctcgacgcc
      301 gtactcgcat cgaacggcgg tgggttcgat ttcaccctcg gggcgatcag caacgacgtc
      361 gccagcaact ccaccacgct gtggtgcttg gactggtacg ccgatgcgcc cggcttcgcg
      421 gtcgtccacc gccaagaagt gctcgatcag tggccgcagc tgatcagcgt cgaggcgttc
      481 cgcaacaatc ccgagttggt cgccaacctc tcggtgaggt gccaatgaag ctcaagctga
      541 cgctcagcgc tggccagcag cacgacgaca tcgnnnnnnn nnnnnnnnnn nnnnnnnnnn
      601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngaccccgg ccgcggtcgc
      661 ggggccgacg ctgacgctgc ggctcaaccc gcatcaccac cagtcgcgag tgatcgcggc
      721 cgacatggtg ctgagcgacg cgggcctgcg caacggcgac gtgatcgagt tgaccaacac
      781 cgtcggcgcc gccgcgtcgt caggcgaggt ggccgccacg ctgacggtgg tcggcggacc
      841 cgacgtcggt cgctcgttcc cgctgccgtc gggcacgagt tcgatcggtc gcgagcgcac
      901 ctcgacggtg cgcctgagcg acctgctggt gtccaagcac cacgccaagg tcaacgtcac
      961 cgcagcgctt gcccgagacg ctgcgcgaac ggcgtaccgc ggaccagcca cggccgtcaa
     1021 tgcggtcgtt gcagcgggtg gtgctgcgcc cgggcgacac ggtgaccatc ggcgagacca
     1081 cgctgtcggc cgtgctgcac cgcaccaccg gaggcacgcg cgtcgcaggc ggtcactcga
     1141 tcgccttcaa ccgctcgccc cgcctcgacc cccgctacgc gggtgccgag ctgatcgctc
     1201 ccgatccgcc acaacgtccc gggcaccagc gcttcccgct gatgaccgtg atcgccccga
     1261 tcctgatggg cgcgatcatg tactcggtca ccaagaacgt gctctcgatc ttgttcgtcg
     1321 ggctctcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnagaac
     1381 cgctggtcga ccgggcggtt gatgaccgca gcgacggcag gcttccgggc ctcgctgcgc
     1441 gaccttgccg tacaggcgca gtacgcggcc gaacaagaac gggtcgggcg gcgagccgag
     1501 cacccctcga tcgaagagat cgtcgatgcc acgcaccgtt tgagcccgct ggtctggact
     1561 cgacgaccga gctatcggtc cttcgccaca gtccgcctcg gcctgggtga ccagccgtcg
     1621 cgcaacacca tcgagaccac ccgcaccaac aacacgctgc ccgagttgtg gcgcgagatg
     1681 cacgacgtgg tgcgccaatt c
//
LOCUS       DAORNF010000762         1701 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_50723_length_1701_cov_1.671324, whole genome shotgun sequence.
ACCESSION   DAORNF010000762 DAORNF010000000
VERSION     DAORNF010000762.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50723_length_1701_cov_1.671324"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            131..>672
                     /locus_tag="PLV68_21035"
     CDS             131..>672
                     /locus_tag="PLV68_21035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03794.1"
                     /translation="MSPLTSVVIAGALVGGGLWLMWTGWVPSHPSLRWALDRLGQPVP
                     EVEPTSRETLDARLGSKVRKIGLIDQAVLAMRTDLRVLRRSPDDQAAILVAYGLLGFL
                     WAPIVAAGGWLVGFQLPLAIPLWLAIAGAGIGVVTAIRSTKAAAAQRRHTFSHALSSF
                     CDVTGMCLAAGRGVESSLETA"
     assembly_gap    673..705
                     /estimated_length=33
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <706..1012
                     /locus_tag="PLV68_21040"
     CDS             <706..1012
                     /locus_tag="PLV68_21040"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HRE03795.1"
                     /translation="TALRTGYVRGDTPWEALARLGDDADLPDLTELAAAVSLAGQEGA
                     AVRNTVTSKAKAIRERLTADAERSAASTTERMGVPATLLLLGFIVFLGFPAIAVLFE"
     gene            1048..1248
                     /locus_tag="PLV68_21045"
     CDS             1048..1248
                     /locus_tag="PLV68_21045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03796.1"
                     /translation="MPTIVHRLCAWLHVQAAEARERAIREADRGEVVEKVIIVAAAAA
                     IAIAAMAAIAAAVDLKIGGLQL"
     gene            1398..>1701
                     /locus_tag="PLV68_21050"
     CDS             1398..>1701
                     /locus_tag="PLV68_21050"
                     /inference="COORDINATES: protein motif:HMM:NF019428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03797.1"
                     /translation="MILLAIVQIGVYFHLRAVAQTAARHGLDEVRVVHGSSEDGIAAA
                     NAFLDQSGSGIDNRTVTANRTATVATVTVSGNAVSIVPGLTLTIEVAVDAPVERVTP"
BASE COUNT          255 a          638 c          520 g          255 t
ORIGIN      
        1 cggccctcgg ccaggtcgtc ctcgccgtcg tcgctctcta ctgggcggcc gggttctggt
       61 ggatgcaccg gatgggccgc gctgcgccac ccgagcggtt cctcgcccca ccggcaacag
      121 agacgccggc atgagcccgc tcacttccgt cgtcattgcc ggcgccctgg tcggtggcgg
      181 gctgtggctg atgtggaccg gctgggttcc atcgcacccg tcgctgcggt gggcgctcga
      241 ccggctcggt caacccgtac ccgaggtcga gccgacctcg cgcgagaccc tcgacgcgag
      301 gctcggctcg aaggtccgca agatcggcct gatcgaccaa gccgtgctgg cgatgcgcac
      361 cgacctgcgc gtcttgcgcc gatcacccga cgaccaggcc gccatcctcg tcgcctacgg
      421 gctactcggg ttcctctggg ctccgatcgt ggccgctggt ggctggctgg tcggcttcca
      481 actccccctc gcgataccgc tgtggcttgc catcgccggg gcgggcatcg gcgtcgtcac
      541 cgcgatccga tcgaccaagg ccgccgcagc gcagcggcgg cacaccttct cgcatgccct
      601 gtcgtcattc tgcgacgtca ccgggatgtg cctcgccgcg ggccgcggcg tcgaatcgtc
      661 actcgagacc gcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnaaccg cgttgcgcac
      721 cggatacgtc cgcggcgaca ccccctggga ggcgctcgct cgactcggcg acgacgccga
      781 cctgcccgac ctcaccgaac tcgcggccgc ggtctcactc gccgggcaag agggcgcggc
      841 ggtgcgcaac accgtgacct ccaaagccaa agcgatcaga gaacgtctca ccgccgacgc
      901 tgaacggtcg gcagccagca ccaccgagcg gatgggcgtc cccgcgaccc tgctgctgct
      961 cggcttcatc gtcttcctcg gtttccccgc aatcgcagtc ctattcgagt aggagccacg
     1021 acccgaccaa ccatcaagga gcaacccatg cccaccatcg tccaccgact ctgcgcctgg
     1081 ctacacgtgc aggccgccga ggcgcgcgag cgagccatac gcgaggccga ccgcggtgaa
     1141 gtcgtcgaga aggtcatcat cgtcgcggcc gccgcggcca tcgcgatcgc agccatggcg
     1201 gccattgccg cggccgtgga tctcaagatc ggcggactcc aactctgact cgcgccggcc
     1261 caccccgacc cgcacgcacg ccacggcgaa gcggacgtcg acagcgtgcg tcagcgcgcg
     1321 ctcgtccaca gcgtcgttgg cgtgacgacc ggggttcggc cactgtcgag ttcgtcatcg
     1381 ctgccgcgct gatggtcatg atcctgctcg cgatcgttca gatcggcgtc tacttccacc
     1441 tacgcgccgt cgcccaaacg gctgcccgtc acggcctcga cgaggtacgg gtcgtccacg
     1501 gctcatccga agacggcatc gccgccgcca acgcgttcct cgaccagagc ggcagcggca
     1561 tcgacaaccg caccgtcaca gccaaccgaa ccgccacagt cgcaacggtc acggtctctg
     1621 gcaacgccgt ctcgatcgtc cccgggctca cgctcaccat cgaggtggca gtcgacgcac
     1681 cggtcgaacg ggtgacgcca t
//
LOCUS       DAORNF010000763         1698 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_50833_length_1698_cov_1.676811, whole genome shotgun sequence.
ACCESSION   DAORNF010000763 DAORNF010000000
VERSION     DAORNF010000763.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50833_length_1698_cov_1.676811"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="PLV68_21055"
     CDS             <1..373
                     /locus_tag="PLV68_21055"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03798.1"
                     /translation="SVVASEALNIVASEALKIVASEALNIVEGVVLSSDVFYPHEVLG
                     SELAKWARTGVVAVEMECAALFITAALHGIQAGAVLTADGNPLAEADSDMSGYDPHRQ
                     VVRDAVDVMIGLGLDVVALPL"
     gene            387..1028
                     /locus_tag="PLV68_21060"
     CDS             387..1028
                     /locus_tag="PLV68_21060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRE03799.1"
                     /translation="MAAPSFMQRKRDELRQRGIDPDRLPPGQYVTDRFPVLHIGPVPA
                     YATLDDWTLAIGGDAVAQPRVLRWAELLELCTADVTVDIHCVTKWSKFDVTWRGVMLA
                     DLLGPSGPRAEARTLIARGEHGYSANLDYAEVCANTALVAVACDGEPLAPEHGYPARL
                     VIPHLYFWKSVKWLRGIELWSDERIGFWEQNGYHEHGDPFREQRYWGDDGEQF"
     gene            1015..1488
                     /locus_tag="PLV68_21065"
     CDS             1015..1488
                     /locus_tag="PLV68_21065"
                     /inference="COORDINATES: protein motif:HMM:NF017936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRE03800.1"
                     /translation="MNNSEALTADEVAALLDLEPLPYEGGWFRRTFADANCSTIIYLM
                     AAGDGFSAMHRLDGPEILTFAAGAPARNLLLHPDGTVSERILGADLAAGHVPQVVVET
                     GVWQGTATLGAWTLITATMAPPYEQEMFHLGRRDDLTARWPDAAGLIARLTRDKH"
     gene            1492..>1698
                     /locus_tag="PLV68_21070"
     CDS             1492..>1698
                     /locus_tag="PLV68_21070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03801.1"
                     /translation="MPATAHQITRPIATMIETALATPTGSVPGDIARLRDTLAVALRR
                     CLDRPTADWGELVAAAAGHGGWDQW"
BASE COUNT          268 a          597 c          576 g          257 t
ORIGIN      
        1 gtccgttgta gccagcgaag cgctcaacat cgttgccagc gaagcgctca agattgtggc
       61 cagcgaagcg ctcaatatcg tggagggcgt cgtgctgagc agcgacgtgt tctacccgca
      121 cgaggtgctt ggcagcgagc tcgcgaagtg ggcgcgcacc ggggtcgtcg cggtcgagat
      181 ggagtgcgca gcgctgttca tcaccgccgc gctgcacggc atccaagccg gtgcggtgct
      241 gaccgcggac ggcaacccgt tggccgaggc cgactccgac atgtccggtt acgaccccca
      301 ccgccaggtc gtgcgcgacg cggtcgacgt gatgatcggg ctcggcctcg acgtcgtggc
      361 cctgccactc tgagagcagc agacccgtgg ccgcaccgtc gttcatgcag cgcaagcgcg
      421 acgagttgcg ccagcgcggc atcgatccag atcggctgcc acctggtcag tacgtgaccg
      481 atcgcttccc cgtgctgcac atcggcccgg tgcccgccta cgccaccctc gacgactgga
      541 cgttggccat cggcggcgac gcggtcgctc agccgcgtgt gctgcggtgg gccgagttgc
      601 tcgaactctg cacggccgat gtcacggtcg acatccactg cgtcaccaag tggtcgaagt
      661 tcgacgtcac ctggcggggc gtgatgctgg ccgacctgct cggacccagc ggtccacgcg
      721 ccgaggcgcg cacgctgatc gcccgcggcg agcacggcta ctcggccaac ctcgactacg
      781 ccgaggtgtg cgccaacacc gcgctcgtcg cggtcgcgtg cgacggcgaa ccgctcgcgc
      841 ccgagcacgg ctatccggcg cggttggtga tcccgcacct gtacttctgg aagtcggtca
      901 agtggctgcg cggcatcgag ctgtggtccg acgaacggat cgggttctgg gagcagaacg
      961 gctaccacga acacggcgac ccgttccgcg agcagcgcta ttggggagac gacggtgaac
     1021 aattctgaag cactgacagc ggacgaggtc gccgcgttgc tcgatctcga accactgcca
     1081 tacgagggcg gctggttccg gcgcaccttc gccgacgcca actgttcgac gatcatctac
     1141 ctcatggcgg cgggcgacgg cttctccgcg atgcaccgtc tcgacgggcc ggagatcctg
     1201 acgttcgcgg ccggagctcc ggcacgcaac ctgctgctgc accccgacgg cacggtgtcc
     1261 gaacgcatcc tcggcgccga cctcgccgcc gggcacgtgc cgcaggtggt ggtcgagacc
     1321 ggggtctggc agggcacggc cacgctcggt gcgtggacgc tgatcaccgc cacgatggcg
     1381 ccgccgtacg agcaggagat gttccacctc ggccggcgtg acgatctcac agcacgctgg
     1441 ccggacgcgg ccgggttgat cgcacggttg acgcgcgaca agcactgagc catgcccgcg
     1501 accgcccacc agatcacgcg cccgatcgcg accatgatcg aaaccgcgct ggcgactccg
     1561 accggatcgg tgccgggcga catcgcccgg ctccgtgaca cactcgccgt cgcgctgcgg
     1621 cgttgcctcg accgccccac cgcagactgg ggcgaactcg tcgctgccgc cgccgggcac
     1681 ggcggctggg accagtgg
//
LOCUS       DAORNF010000764         1697 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_50871_length_1697_cov_1.878806, whole genome shotgun sequence.
ACCESSION   DAORNF010000764 DAORNF010000000
VERSION     DAORNF010000764.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1697
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50871_length_1697_cov_1.878806"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>635)
                     /locus_tag="PLV68_21075"
     CDS             complement(<1..>635)
                     /locus_tag="PLV68_21075"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HRE03802.1"
                     /translation="DGARAAGLALSPVESFANTQGLGVSGVVDGHAVVAGRESFLADH
                     ALHLDDDLRAAKDAAEAAGRTAIVVGWDGVLGGVVVVADTVKPTSAAAIAELRRLGLR
                     PMLLTGDNRRAAHNVAAQVGIDPADVIAEVLPRDKVDVVARLQGEGAIVAMVGDGVND
                     SAALAQADLGLAMGTGTDAAIEASDLTLVRGDLGAAPDAIRLSRRTLTTIK"
     assembly_gap    636..655
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<656..>1697)
                     /locus_tag="PLV68_21080"
     CDS             complement(<656..>1697)
                     /locus_tag="PLV68_21080"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HRE03803.1"
                     /translation="PVVTWGAWPFHRAAWINLRHAAATMDTLISVGVLAAFGWSLYAL
                     FFGDAGVPDMRHGFSITTDRTMGASMIYLETAAGVTAFILAGRFFEARAKRRSGAALR
                     AMLDLGAKDVAVVRDGTEARIPIGQLVVGDRFVVRPGEKIATDGVVEEGTSAVDTSLL
                     TGEPVPVEVAPGDTVTGATVNAGGRLIVRATQVGADTQLAQIARLVDAAQSGKAPVQR
                     LADRVSGVFVPIVIALAAATLGFWLGTGYDTSVAFTAAVAVLIIACPCALGLATPTAI
                     MVGTGRGAQMGIIIKGPEVLESTRQVDTIVLDKTGTITTGRMALVDVVPAAGIERDRL
                     LQLAGSLEDASEH"
BASE COUNT          247 a          600 c          590 g          240 t
ORIGIN      
        1 ccttgatcgt ggtcagggtg cggcgcgaca ggcggatcgc atcgggcgcg gcaccgaggt
       61 cgccccgcac cagggtgaga tcgctggctt cgatcgcagc gtcggtgccg gttcccatcg
      121 ccaggccgag gtcggcttgg gccagcgcgg ccgagtcgtt gacgccgtcg ccgaccatcg
      181 ccacgatggc accctcacct tgcaggcggg ccacgacgtc gaccttgtcg cgggggagga
      241 cctcggcgat cacgtcggcc gggtcgatgc cgacctgggc cgcgacgttg tgggcggcgc
      301 gtcggttgtc gccggtcagc agcatggggc gcaggcccaa gcggcgcagc tcggcgatcg
      361 ctgccgcgct ggtgggcttg acggtgtcgg ccaccacgac cacaccgccc agcacgccgt
      421 cccagccgac gacgatcgcg gtgcgcccgg cggcttcggc tgcatccttc gcagcgcgca
      481 ggtcgtcgtc gaggtggagc gcgtgatcgg ccaggaacga ctcgcgtccg gcgaccacgg
      541 cgtgaccgtc gaccacgccg ctgacgccga ggccctgcgt gttggcgaag ctctcgaccg
      601 ggctcagtgc caaaccggcc gcacgcgcgc cgtcgnnnnn nnnnnnnnnn nnnnngtgct
      661 cggacgcgtc ttcgagcgat cctgccagtt gcaacaaccg gtcgcgttcg atgccggccg
      721 cgggtacgac gtcgaccagg gccattctcc cggtggtgat cgtgccggtc ttgtcgagca
      781 cgatcgtgtc gacctggcgg gtcgactcca gcacctcggg acccttgatg atgatgccca
      841 tctgcgcgcc ccgtccggtg ccgaccatga tcgcggtcgg cgtggccagg ccgagcgcgc
      901 acgggcaggc gatgatcaac acggccacgg ccgcagtgaa cgcgaccgac gtgtcgtagc
      961 cggtgccgag ccagaacccc aacgtcgccg ccgcgagggc gatcacgatc gggacgaaca
     1021 cgcccgagac ccgatcggcc aatcgctgca caggtgcctt gccggactgg gccgcgtcga
     1081 ccaggcgggc gatctgggcc agttgggtgt cggccccgac ctgagtcgcc cgcacgatca
     1141 accgcccacc ggcgttgacc gtcgctccag tgaccgtgtc gccgggagcg acttcgaccg
     1201 gcaccggctc acccgtgagc aacgacgtgt cgaccgcgga cgtgccctcc tcgaccacgc
     1261 cgtcggtcgc gatcttttcg ccggggcgga cgacgaaccg gtcaccgacc acgagttgcc
     1321 cgatcggaat gcgcgcctcg gtgccgtcgc gcacaacggc gacgtctttc gcgccgaggt
     1381 cgagcatcgc ccgcagtgcc gcgcctgagc ggcgcttggc ccgagcttcg aagaaccgcc
     1441 cggcgaggat gaacgcggtg accccggccg ccgtttcgag gtagatcatg ctcgcaccca
     1501 tcgtgcggtc ggtggtgatg ctgaacccgt gccgcatgtc gggcacgccc gcgtcgccga
     1561 agaacagcgc gtacagcgac cagccgaacg cggccaacac gccgaccgag atcagcgtgt
     1621 ccatcgtggc tgcggcgtgg cgcaggttga tccatgccgc gcggtggaac ggccatgcgc
     1681 cccaggtgac caccggg
//
LOCUS       DAORNF010000765         1695 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_50948_length_1695_cov_1.771341, whole genome shotgun sequence.
ACCESSION   DAORNF010000765 DAORNF010000000
VERSION     DAORNF010000765.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50948_length_1695_cov_1.771341"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            43..>1067
                     /gene="hisS"
                     /locus_tag="PLV68_21085"
     CDS             43..>1067
                     /gene="hisS"
                     /locus_tag="PLV68_21085"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HRE03804.1"
                     /translation="MPEFQTSPGMRDILPPDSTRWRRFQAVFADVVRAAGYAEIMPPM
                     LEDLGVFQRIGDATDVVTKEMFDFTDRGGRHIALRPEQTASVCRAFVQHRPLTPWKVY
                     YAGPNFRYEKPQRGRYRQFDQVGIEVLGADDPYLDVEVIALAWEFYGRLGLTQVDLLL
                     NSLGEPEDRAAYVAALEQYFRDHLDALSPESQATLAKNPLRVLDSKRDQDHAIVAAAP
                     QIADFYSPDAAAHFDAVQQGLTDLGIPFRIDPKLVRGLDYYRRTTFEFQARTLDSAQN
                     AVGGGGRYDGLVESLGGPATHGIGFALGLDRTLLACDDEGVFAAPSTAVDVFVVDTTG
                     GLEALRLT"
     assembly_gap    1068..1087
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1088..1322
                     /locus_tag="PLV68_21090"
     CDS             <1088..1322
                     /locus_tag="PLV68_21090"
                     /inference="COORDINATES: protein motif:HMM:NF015113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HRE03805.1"
                     /translation="AGISADRAYENRSMKSQMKVADRSGAEVAVIIGTNELDAGTAVV
                     RPLRGADQQREVGRATLITDVRQALDAARKATP"
     assembly_gap    1380..1412
                     /estimated_length=33
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1413..>1695
                     /locus_tag="PLV68_21095"
     CDS             <1413..>1695
                     /locus_tag="PLV68_21095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HRE03806.1"
                     /translation="RREHGSQLAFVDVRDHTGVIQCVVDNDVDVRSEYVLRITGVVRD
                     RLEGTVNPNLPTGEVELGECNVEVLRQAEPPPFPIDARADKVEETTRLQY"
BASE COUNT          266 a          580 c          552 g          244 t
ORIGIN      
        1 ggggcggggg actgggtcgg ggcggcatta gcctgtggac tcgtgcctga gttccagacc
       61 agcccgggaa tgcgggacat cctgccgccc gactcgactc gctggcggcg gttccaggcg
      121 gtgttcgcgg atgttgtccg agccgctggt tatgccgaga tcatgccgcc gatgcttgag
      181 gatctgggcg tattccagcg catcggcgac gcgaccgacg tggtcaccaa ggagatgttc
      241 gacttcaccg accgcggcgg gcgccacatc gccttgcgcc ccgagcagac cgccagcgtg
      301 tgccgggcgt tcgtgcaaca ccgcccgctg acgccgtgga aggtgtacta cgccggtccc
      361 aacttccgct acgagaagcc gcagcgaggc cgctatcgcc agttcgatca ggtcggcatc
      421 gaggtgctcg gcgccgacga tccgtacctc gacgtcgagg tcatcgcgtt ggcgtgggag
      481 ttctacggcc gtctcgggct gacccaggtc gatttgttgc tgaacagcct cggagaaccc
      541 gaagaccgcg ccgcctacgt cgccgcgctc gagcagtact tccgcgacca cctcgacgcg
      601 ctcagccccg agagccaggc cacgctggcc aagaacccgc tgcgggtgct cgactccaag
      661 cgcgaccagg accacgcgat cgtcgctgcc gcgccgcaga tcgccgactt ctacagcccc
      721 gacgcggccg ctcacttcga cgcggtgcag cagggcttga ccgacctggg cattccgttc
      781 cgcatcgacc caaagctggt gcgcggcctc gactactacc gccgcaccac gttcgagttc
      841 caggcgcgca cgctcgactc ggcccagaac gccgtgggtg gcgggggccg ttacgacggc
      901 ttggtcgagt cgctgggtgg cccggccacg cacggcatcg gctttgcgct cggcctcgac
      961 cgcaccctgc tcgcctgcga cgacgagggc gtgttcgccg cgccgtcgac cgcggtcgac
     1021 gtgttcgtgg tcgacaccac cggcgggctc gaagcgctgc gcctcacnnn nnnnnnnnnn
     1081 nnnnnnncgc gggcatctcg gccgatcggg cctacgagaa ccgcagcatg aagagccaga
     1141 tgaaggtggc cgaccgtagc ggtgccgaag tcgccgtcat catcggcacc aacgagctcg
     1201 acgcgggcac cgccgtggtg cgtccgctgc gcggcgccga ccagcaacgc gaggtcggcc
     1261 gcgccaccct gatcaccgac gtccggcagg cactcgacgc cgccaggaag gcaactccat
     1321 gagcgctacc tcgatgcgca cgcacatgaa cggcgagctg cgccgcagca acgtcggcgn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncgccgtga gcacggctcg caactggcct
     1441 tcgtcgacgt ccgcgatcac acgggtgtga tccagtgcgt cgtcgacaac gacgtcgacg
     1501 tgcgcagcga gtacgtgctg cgcatcacgg gtgtggtccg cgaccggctg gagggcacag
     1561 tcaaccccaa cctgcccacg ggtgaggtcg aactggggga gtgcaacgtc gaggtgttgc
     1621 gccaggccga gccgccgccc ttcccgatcg acgctcgcgc cgacaaggtc gaagagacca
     1681 cgcggctgca gtacc
//
LOCUS       DAORNF010000766         1692 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_51045_length_1692_cov_1.614539, whole genome shotgun sequence.
ACCESSION   DAORNF010000766 DAORNF010000000
VERSION     DAORNF010000766.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51045_length_1692_cov_1.614539"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>245)
                     /locus_tag="PLV68_21100"
     CDS             complement(<1..>245)
                     /locus_tag="PLV68_21100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRE03807.1"
                     /translation="REEKAAYAPQDSEFIISHGMCVPMLAEFGTHEQKQQFLADAIDG
                     TTMWCQMFSEPGAGSDVASLQSRAELDGDEWVINGQKV"
     assembly_gap    246..276
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<277..479)
                     /locus_tag="PLV68_21105"
     CDS             complement(<277..479)
                     /locus_tag="PLV68_21105"
                     /inference="COORDINATES: protein motif:HMM:NF014790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRE03808.1"
                     /translation="MTDTTITATVDLDGTADLDSFRQRCREFLAANHKEAGEIDFAWN
                     KEFLKRSAAAGLAGIPYPSEYGGG"
     gene            627..>829
                     /locus_tag="PLV68_21110"
     CDS             627..>829
                     /locus_tag="PLV68_21110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03809.1"
                     /translation="MATTHELDAPPWSRVESTLMSTARALREAYDTQFAPLGLNLTQA
                     SLLAYLREFGPVTQTKCADHLRLG"
     assembly_gap    830..879
                     /estimated_length=50
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <880..1080
                     /locus_tag="PLV68_21115"
     CDS             <880..1080
                     /locus_tag="PLV68_21115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03810.1"
                     /translation="VERHPDADDRRVWQVALSADGEALVDQILAIDEDFRAQLRDGIT
                     RAERQALASVMTRMQANLARIS"
     gene            1149..>1310
                     /locus_tag="PLV68_21120"
     CDS             1149..>1310
                     /locus_tag="PLV68_21120"
                     /inference="COORDINATES: protein motif:HMM:NF012336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="steroid 3-ketoacyl-CoA thiolase"
                     /protein_id="HRE03811.1"
                     /translation="MTDAVIIDAIRTPGGKRNGKLKDWHPAALAAHVLKALAERNNLD
                     PAKVDDVIMG"
     assembly_gap    1311..1369
                     /estimated_length=59
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1370..>1692
                     /locus_tag="PLV68_21125"
     CDS             <1370..>1692
                     /locus_tag="PLV68_21125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="steroid 3-ketoacyl-CoA thiolase"
                     /protein_id="HRE03812.1"
                     /translation="VPATTVDRQCGSSQQAMHFAAQGVIAGAYDIAVAAGVEVMSTTP
                     MGASVVGGTMGHPFPKEQRDRYAETGLPPQGIGADMIADQWGLTREDLDAFGALSQQR
                     AETAT"
BASE COUNT          271 a          572 c          476 g          233 t
ORIGIN      
        1 accttctggc cgttgatcac ccactcgtcg ccgtcgagct cagctctact ctgcaagctc
       61 gccacgtcgc tgccagcacc cggctcggag aacatctggc accacatggt ggtgccgtcg
      121 atcgcgtcgg ccaggaactg ctgcttctgt tcgtgcgtgc cgaactcggc cagcatcggc
      181 acgcacatgc cgtggctgat gatgaactcg ctgtcctggg gcgcatacgc cgccttctct
      241 tcccgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncccc cgccgtactc ggacgggtag
      301 gggatgcccg ccaggccagc ggcagcagaa cgcttgagga actccttgtt ccaggcgaag
      361 tcgatctcac cagcctcttt gtggttggcg gccaggaact cacggcaacg ctgccggaag
      421 ctgtcgagat cagcggtgcc atcgagatcg acggtggctg tgatggtcgt gtcggtcatc
      481 gcgttctccg ggtggtgcgt cgcatcatgc tcgatgtgcg gtcatattgt atagcgtcgt
      541 atagtatgac ggaaagcaac atccgccgac gagcccgagg ggaggacgag cgcacgatgc
      601 cgaaccgagc gacggacaca cgcagcatgg cgaccactca cgagctcgac gctcccccgt
      661 ggtcgcgggt cgaatcgacg ttgatgagca ccgcccgcgc cctgcgcgag gcctacgaca
      721 cgcagttcgc tcccctcggc ctcaacctca cccaggccag cctgctcgcc tacctgcgcg
      781 agttcggacc cgtcacccag accaagtgcg ccgaccacct gcgcctcggn nnnnnnnnnn
      841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng tcgagcgcca cccagatgcc
      901 gacgaccgcc gggtgtggca ggtcgcgctg tcggccgacg gcgaggccct cgtcgaccag
      961 atcctggcga tcgacgagga ctttcgtgcc caactccgcg acggcatcac ccgcgccgaa
     1021 cgccaggcgc tcgcctcggt gatgacccgg atgcaggcca acctggcccg catctcgtag
     1081 ttcccccctc gactgaccca ccaccccaac caacccaacg aacctcggct acccctggag
     1141 acaccaccat gaccgacgca gtgatcatcg atgccatccg cacccccggc ggcaagcgca
     1201 acggcaagct caaggactgg catccggcgg ccctggccgc ccacgtgctg aaggcgttgg
     1261 ccgagcgcaa caacctcgac ccggccaagg tcgacgacgt catcatgggt nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng gtgccggcca
     1381 ccaccgtcga ccgccagtgc ggcagcagcc agcaggcgat gcacttcgcc gcgcagggcg
     1441 tcatcgccgg tgcctacgac atcgccgtcg cggccggcgt cgaggtcatg tcgaccacgc
     1501 cgatgggcgc gtccgtggtt ggcggcacca tgggccaccc cttccccaag gagcagcgcg
     1561 accgctacgc cgagacgggc ttgccgccgc agggcatcgg cgccgacatg atcgccgacc
     1621 aatggggcct cacccgcgaa gacctcgacg cgttcggggc gctcagccaa cagcgggccg
     1681 agaccgccac cg
//
LOCUS       DAORNF010000767         1685 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_51296_length_1685_cov_1.515951, whole genome shotgun sequence.
ACCESSION   DAORNF010000767 DAORNF010000000
VERSION     DAORNF010000767.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1685
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51296_length_1685_cov_1.515951"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            12..>327
                     /locus_tag="PLV68_21130"
     CDS             12..>327
                     /locus_tag="PLV68_21130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03813.1"
                     /translation="MATIGGLVLVGVSAGEETASMLSRDVKFGIVLGVPVLALVGAVA
                     KRMRHSIALASLAGLAFTASALAARAVRIRGQSVAHILTSPLAWAVLLYAFLLLFAPM
                     AAI"
     assembly_gap    328..393
                     /estimated_length=66
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <394..604
                     /locus_tag="PLV68_21135"
     CDS             <394..604
                     /locus_tag="PLV68_21135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03814.1"
                     /translation="VLGDHSRPGWELAAIGGILVTLAATVVLATAPAQDLEHRELAPL
                     LDPLLDAMPDPAPDPTFGPAPDPDD"
     gene            635..>1685
                     /locus_tag="PLV68_21140"
     CDS             635..>1685
                     /locus_tag="PLV68_21140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRE03815.1"
                     /translation="MTATNTDAESQLVEQALEALLAAHDPSAESYHEFRGHQYDAGLA
                     WVHFPVGFGGLGVRPDLQKVVERRVRAAGAKSADPASFFLALAGPTIVTHGSDEQRRR
                     FLRPMFTGEERWCQLFSEPGAGSDFAGLAARAVRDGDEWVVNGQKVWNTIAHLADWGM
                     LVTRSNPEAPKHKGMTYFAIDMHSPGVEVRPLRQITGEAEFNEVYLTDARVPDANRIG
                     AEGEGWRVSLTTLMNERATIGGGSGGTPKRGSGAIGELVDLWTALPADQRDPARRDQV
                     MRMWVRAECLRLTNIRAGGNRKAGNPGPEGSIAKLALAELNKELFEVAIDLLGADGMI
                     GYDYTFRRPESLSTEG"
BASE COUNT          248 a          572 c          548 g          251 t
ORIGIN      
        1 agtgggcgat catggccacg attggcgggc tggtgctggt gggcgtctcc gctggcgaag
       61 agaccgccag catgctcagt cgcgacgtca agttcggcat cgtgctcggt gtgcccgtgc
      121 tggccttggt cggcgcggtc gccaagcgga tgcggcactc gatcgcgctg gcgagcctcg
      181 ccggtcttgc gttcaccgcg agcgcgctcg cggcccgggc ggtacgcatc cgcggccagt
      241 cggtggccca catcctcacc tcgccactgg cctgggcggt gctgctctac gcctttttgc
      301 tcctgtttgc gccgatggcg gcgattgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncgtactc ggcgaccaca gtcggcctgg
      421 gtgggagctg gccgccatcg gcggcatcct cgtcacgttg gccgccacgg tggtgctggc
      481 caccgcgccc gcccaggacc tcgagcaccg cgaactggcg ccgctgctcg atccgctgct
      541 cgacgcgatg cccgatccag cacccgaccc gacgtttggc ccggcgccag atcctgatga
      601 ctgaatccgc gccgcccgac gccagaatgt cgtcatgacc gcaacgaaca ccgacgccga
      661 gtcgcagctc gtcgagcaag ccctcgaagc gctgctagcc gcacacgacc cctccgccga
      721 gagctaccac gagttccgcg gacatcagta cgacgctggg ttggcatggg tgcacttccc
      781 cgtcggtttc ggaggcctcg gcgtgcggcc cgatctgcag aaggtggtcg aacggcgcgt
      841 gcgcgcggcc ggtgccaaga gcgccgaccc ggcctcgttc ttcctggccc tcgccgggcc
      901 gacgatcgtc acccacggct ccgacgagca acgacgccgg ttcctgcgcc cgatgttcac
      961 cggcgaagag cggtggtgcc agctgttcag cgaacccggt gcgggcagcg acttcgccgg
     1021 cctcgccgcc cgcgcggtgc gcgatggcga cgaatgggtg gtcaacggcc agaaggtgtg
     1081 gaacaccatc gcccacctgg ccgactgggg catgttggtc acccgcagca accctgaggc
     1141 gcccaagcac aagggcatga cctacttcgc gatcgacatg cacagccccg gggtcgaggt
     1201 ccgccccctg cgccaaatca ccggcgaggc cgagttcaac gaggtctacc tcaccgacgc
     1261 acgcgtgcct gacgccaacc gcatcggcgc cgagggcgag ggctggcgag tgtcgctgac
     1321 cacgttgatg aacgagcgag ccaccatcgg cggtggcagc ggtggtacac ccaagcgtgg
     1381 atcgggtgcg atcggcgaac tggtcgatct gtggacggcg ctgcccgccg accagcgtga
     1441 cccggcccgc cgcgaccagg tcatgcgtat gtgggtccgg gccgagtgct tgcggctcac
     1501 caacatccgc gctggcggca accgcaaggc gggcaacccc gggcccgagg gttcgatcgc
     1561 caagctggct ctggccgagc tcaacaagga gttgttcgag gtggcgatcg acctgctcgg
     1621 tgccgacggc atgatcggct acgactacac cttccgtcgc cccgagtcgc tgtcgaccga
     1681 aggca
//
LOCUS       DAORNF010000768         1684 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_51335_length_1684_cov_1.980970, whole genome shotgun sequence.
ACCESSION   DAORNF010000768 DAORNF010000000
VERSION     DAORNF010000768.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51335_length_1684_cov_1.980970"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..972
                     /locus_tag="PLV68_21145"
     CDS             <1..972
                     /locus_tag="PLV68_21145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03816.1"
                     /translation="VDPRVDPLVERPGERPSGSTPRQGTPATAGRDVASRQPASRQAT
                     PRQGTPRQDQSNQSAARFDTPRSGTPRQGPARQIKTTTPRSGSRDRTPNGVTPRGVPA
                     RQFQQHRGPQVVIAILLLLAAVVALALWVTRDTTSGTPFTPDQSGPVDAQSQDATGDA
                     TESEQTTPGPVSPAVPATILGVTSFGDEHPDEAVLAIDGDTGTGWTTVCYNDKYFNGK
                     PGVGLIAQMSGPATGRATIAISSAPFQLDVYASDADVAPTTIGGWGAKVVSQANGSEP
                     GTVDAQIEGARWVLALLREAGTGDNGCTTKFPYQGAISEIRFTNAAD"
     gene            959..>1393
                     /locus_tag="PLV68_21150"
     CDS             959..>1393
                     /locus_tag="PLV68_21150"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HRE03817.1"
                     /translation="MQPTDDRELVAAAQGGDRASLDALLRRHYDRIFAVCRRVTGNEA
                     DGADAAQEALIAIVRGLPRFDGRSSFSTWIYRIATNASLDELRRRRRRPVLDGGRHDR
                     GHDDDMQPVEYADPNATVRIDSLGDRLELDTVLADLAEEFRLP"
     assembly_gap    1394..1431
                     /estimated_length=38
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          288 a          641 c          508 g          209 t
ORIGIN      
        1 gtcgatcccc gcgtcgatcc gctggtcgaa cgaccgggcg aacggccgtc gggttcgacg
       61 ccgcgccaag gcacaccggc caccgccggc cgcgacgtcg cgtcgcgtca gcccgcgtca
      121 cgacaagcaa ccccccggca gggcacgcct cgccaggacc agtcgaacca gtcagcggcg
      181 cggttcgaca cgccacgcag cggcacgccg cgccaagggc cagcgcgcca gatcaagacg
      241 acgacgccac gatcaggctc tcgcgatcgc acccccaacg gggtgactcc ccgcggggtg
      301 cccgccaggc agttccagca acaccgtgga ccccaggtgg tgatcgcgat cttgttgctg
      361 ctcgcagccg tcgtggccct cgcgctgtgg gtcacccgcg acaccacgtc gggcacgccg
      421 ttcacccccg accaatcggg ccccgtcgac gcccagtccc aggacgcaac gggtgacgcc
      481 accgaatccg agcagacgac ccccggcccg gtctcccccg ctgtgccggc aaccatcctc
      541 ggcgtcacca gcttcggcga cgagcacccc gacgaggctg tgctcgccat cgatggtgac
      601 acgggcactg gctggaccac ggtctgctac aacgacaagt acttcaacgg caagccgggt
      661 gtcggcctca tcgcgcagat gtcggggccc gccaccggca gggccacgat cgcgatcagc
      721 agcgctccgt tccaactcga cgtgtacgcc tccgacgccg acgtcgcgcc caccaccatc
      781 ggtggatggg gggccaaggt ggtatcgcag gccaacggtt ccgagcccgg caccgtcgac
      841 gcccagatcg agggagcacg atgggtcctg gccctgctgc gcgaggcagg aaccggcgac
      901 aacggatgca ccaccaagtt cccctaccaa ggtgcaatca gcgagatccg cttcaccaat
      961 gcagccgact gacgatcgcg aactcgtcgc agcagcacag gggggtgacc gcgccagcct
     1021 cgatgccttg ctccggcgtc actacgaccg catcttcgcg gtctgccggc gcgtcaccgg
     1081 caacgaagcc gacggggcgg acgcggcaca ggaagcgctg atcgccatcg tgcgcggact
     1141 cccccgcttc gacgggcggt ccagcttctc cacgtggatc taccgcatcg ccaccaatgc
     1201 cagcctcgac gagttgcggc gtcggcgccg tcggccggtg ctcgacggcg ggcgtcacga
     1261 ccgcggccat gacgacgaca tgcagcccgt cgagtacgcc gatcccaatg ccacggtgcg
     1321 gatcgactca ctcggtgatc ggctcgagct cgacaccgtg ctggccgatc tggccgagga
     1381 attccggctc cctnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncgccgagat
     1441 cgcacccgtg ctcgacctgc cgctgggcac ggtgaagagc cgcatcgccc gcggccgcgc
     1501 ccacctcgcc cgcgccctcg accttcgttc gtccgacgac agcgggaacc aaaccagtcc
     1561 gtccacacgt ccaacggata ccccatgaac gacgtgaccc caccccccgg cgaaccgacg
     1621 tccgaacacg agcgcatcag cgccgcgttc gatgacgccc tcgccgcggc gcgagatcac
     1681 gtcc
//
LOCUS       DAORNF010000769         1684 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_51339_length_1684_cov_1.742787, whole genome shotgun sequence.
ACCESSION   DAORNF010000769 DAORNF010000000
VERSION     DAORNF010000769.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51339_length_1684_cov_1.742787"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(169..>558)
                     /locus_tag="PLV68_21155"
     CDS             complement(169..>558)
                     /locus_tag="PLV68_21155"
                     /inference="COORDINATES: protein motif:HMM:NF025698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4395 domain-containing protein"
                     /protein_id="HRE03818.1"
                     /translation="VFGQRWLTIALAYGFVARVVAGPALSPLARVAVWLAPRIAPVRE
                     VPGPPKRFAQSLGALFTVAAAVATYAFDAFGVAKVLLAMLIVPAALEAFAGWCLGCAM
                     FARLMRAGIIPETVCDDIWAQRSVSPG"
     assembly_gap    559..586
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            909..>1684
                     /locus_tag="PLV68_21160"
     CDS             909..>1684
                     /locus_tag="PLV68_21160"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03819.1"
                     /translation="MGRAVELAAFDAILSRADPRNVVHLIGPGGIGKSTLLREVARRA
                     AALGYVVRVLEGRDLPPFPDEIGEALEVGDLGGRPLLMIIDSYELLGSLDGHLRNSVV
                     PSLPDSSVVVLAGRTRPSPGWFDGGWDTVSATVELTGISNAEALTLVRHHGLDDLQVA
                     EGLVHRSGGSPLALVIGAQSGGNGSMAELLDRLLGEEDDPERGRVLGVAALARVTTPE
                     LLEAVLPGNDPHSEYKWLADRSYVEPLADGVALHALVARAL"
BASE COUNT          265 a          604 c          549 g          238 t
ORIGIN      
        1 ccaagcagtg gttggccgat ccgaccgggc ggttcgaact gcgctactgg gacggcttcg
       61 gctggaccga acacgtcgcc accgccgggg tcgcctcgat cgacccgctc gcccaacccc
      121 cgcgctagca cgacggcgcg ttggtcccgc gcccacgact gtggccggtc agcccgggct
      181 gacgctgcgc tgtgcccaga tgtcgtcgca caccgtttcc gggatgatcc cggcacgcat
      241 caagcgggcg aacatcgcac agccgaggca ccaccccgcg aaggcttcca gcgccgcggg
      301 cacgatcaac atcgcgagca acaccttggc cacaccgaac gcgtcgaagg cgtaggtcgc
      361 caccgccgcg gccaccgtga acagggcgcc cagcgactgc gcgaaacgct ttggcggccc
      421 cggcacctcg cgcacgggcg cgatgcgcgg cgccaaccac accgcgacgc gcgccaatgg
      481 actcaacgct gggccggcga cgacacgggc gacgaacccg tacgcgagag cgatggtcaa
      541 ccagcgctgg ccgaacacnn nnnnnnnnnn nnnnnnnnnn nnnnnncccc ggtagccacc
      601 agccgggcgg cgtattcgtt caccacgacg gggaacttca gacgcttcac aggatcacgc
      661 taccgaccct tctccgattg tcgatcattc cgatcggatt tatcatccag ccgtgaccct
      721 cgaccctgac ggaagctcac ctgccgttcg caccgcgtcg ttcgactggg atcgctgagc
      781 gcgcaacgtc ttcacaacgg acgcgcaaca gcagcgtcac cgtccgtgtc gccatcgtca
      841 cgcgactacg atcccgacga atggcaagcc ttcgtgatcg tgtcgagcga aacgatcggg
      901 cggctttcgt gggccgcgcg gtcgagctcg ccgcgttcga tgcgatcctg tcgcgcgccg
      961 acccgcgcaa cgtcgtccac ctcatcgggc cgggcggcat cggcaagagc acgctgttgc
     1021 gcgaagtcgc ccggcgggcg gccgcgctcg gctacgtggt gcgcgtgctc gaagggcgcg
     1081 acctgccgcc attccccgac gagatcggcg aggcgctcga ggtcggcgat ctcggcgggc
     1141 gaccgctgct gatgatcatc gactcgtacg aactgctcgg ttcgctcgac ggtcacctgc
     1201 gcaactcggt cgtgccgtcg ctgcccgact cgtcggtggt cgtcttggca ggtcgcactc
     1261 ggcccagccc ggggtggttc gacggcggtt gggacaccgt gtcggccacc gtcgagctca
     1321 ccggcatctc caacgcggaa gcgctcacgc tggtccgtca ccacggcctc gacgacctgc
     1381 aggtagccga gggcctggtg caccgcagcg gcgggtcacc gctggcgttg gtgatcggcg
     1441 cacagtccgg cggcaacgga tcgatggccg agctgctcga ccgcctgctc ggcgaagagg
     1501 acgaccccga acgaggccga gtgctcggcg tggccgcgct ggcccgggtg accacccccg
     1561 aactgctcga agcggtgctg cccggcaacg acccgcacag cgagtacaag tggctggccg
     1621 atcggtcgta cgtcgagccg ctggccgacg gggtcgcgct gcacgcgctg gtggcccggg
     1681 ccct
//
LOCUS       DAORNF010000770         1674 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_51673_length_1674_cov_1.310068, whole genome shotgun sequence.
ACCESSION   DAORNF010000770 DAORNF010000000
VERSION     DAORNF010000770.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1674
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51673_length_1674_cov_1.310068"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="PLV68_21165"
     CDS             <1..275
                     /locus_tag="PLV68_21165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03820.1"
                     /translation="TARPVSVRSGTGALASERFEQLFGTPHGTDIAALATAHGLTARH
                     AHDDADLRTALAEPGPHVTVVTTDRQANVAVHAALNAAAVAAVDAL"
     gene            complement(315..>1227)
                     /locus_tag="PLV68_21170"
     CDS             complement(315..>1227)
                     /locus_tag="PLV68_21170"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isochorismate synthase"
                     /protein_id="HRE03821.1"
                     /translation="GKLADGRCFVTTIDGATMPTPAPTPLPTAAAFTVRPGVAVDTYL
                     AAVAAARDAVRAGTIDKAVIARDLVVTSPDPIDIHAVMLRLRASFGSSYRYAIDGFVG
                     ASPELLVARDGDIVRSHPLAGTTPRTGDPATDRRLADELLASTKNQIEHRVVIDVVHD
                     TLLPWASYLDWEPEPSIVAVANVQHLGTRLEGRLSQPAPDVVTMARALSPTPALGGHP
                     RDAAIALIERVEGFRRGHYGGAVGWVDAAGNGTWAVAIRCAEFAPDRRSAHLYAGGGI
                     VADSDPHHELAETQAKFQAMLSAIIRP"
     assembly_gap    1228..1251
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1252..1538)
                     /locus_tag="PLV68_21175"
     CDS             complement(<1252..1538)
                     /locus_tag="PLV68_21175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03822.1"
                     /translation="MRAVTTWLDADRRSGEFGPNGVDLNDVAGGDGVLFVRDGVGLAG
                     RGVAARIGADEAVEFLNSLDIDDEVGLTGSGPVALGVLPFRPGAAAKLIVPA"
BASE COUNT          235 a          581 c          604 g          230 t
ORIGIN      
        1 gcacggcgag gccggtcagc gtgcgttccg gtacgggcgc gctggcgagc gagcggttcg
       61 agcaactctt cggcacgccg cacggcaccg acatcgccgc cctcgcgaca gcccacggcc
      121 tgacggcgcg ccacgcccac gacgatgccg atctgcgcac cgccttggcc gagccgggtc
      181 cgcacgtcac cgtcgtcacc accgaccggc aggccaacgt cgcggttcac gccgcgctga
      241 acgcggcggc ggtcgctgcc gtcgacgccc tgtgacccgg cggcggcaat cgggcgctgg
      301 agtgatcacc ccgctcaggg gcggatgatc gcgctgagca tcgcttggaa cttggcctgc
      361 gtctcggcca actcgtggtg cgggtcgctg tcggcgacga tgccgccacc ggcgtagaga
      421 tgggccgagc gacgatcggg ggcgaactcg gcgcagcgga tcgccaccgc ccacgtgccg
      481 ttgcccgccg cgtcgaccca gccgaccgcg cccccgtagt ggccgcggcg aaagccctcg
      541 acccgttcga tcagtgcgat cgcggcgtcg cgtgggtgcc cgcccaaggc gggggtgggg
      601 gagagcgcac gggccatcgt caccacgtcg ggtgcgggct gcgacaggcg tccctccagc
      661 cgggtgccca gatgctgcac gttggcgacg gcgacgatcg acggctccgg ttcccagtcg
      721 aggtagctgg cccaggggag cagcgtgtcg tgcaccacat cgatcaccac gcggtgttcg
      781 atctgattct tcgtgctggc caacagttcg tcggccagac gccgatcggt cgccgggtcg
      841 ccggtgcgtg gcgtggtacc cgcgagtggg tgtgaacgga cgatgtcgcc gtcgcgggcg
      901 acgagcagtt cgggcgacgc gccgacgaag ccgtcgatgg catagcgata gctcgacccg
      961 aagctggccc gcaggcgcag catgacggcg tggatgtcga tcgggtcggg gctggtgacg
     1021 acgagatcgc gggcgatcac cgccttgtcg atcgtgcccg cccgtactgc gtcgcgggca
     1081 gcagcgacag cggcgagata ggtgtcgacc gcgacgccgg gccgcacggt gaacgccgcc
     1141 gcggtgggca acggtgtggg tgcgggtgtc ggcatcgtgg cgccgtcgat cgtggtgacg
     1201 aagcagcgtc cgtcagccag cttgccgnnn nnnnnnnnnn nnnnnnnnnn ngccgggacg
     1261 atcagcttgg ccgccgcacc cggccggaac gggagcacgc ccagcgcaac cggaccgctg
     1321 ccggtgagcc ccacctcgtc gtcgatgtcg agcgagttga ggaactcgac cgcctcgtcg
     1381 gcgccgatcc gcgccgccac gccgcgcccg gccaggccga cgccgtcgcg gacgaatagc
     1441 accccgtcgc cacctgccac atcgttcagg tcgacgccgt tgggcccgaa ctcgccggaa
     1501 cggcggtcgg catcgagcca ggtcgtgacc gcgcgcacgc gtctcagtcc cgtgtcgcga
     1561 cgagcatctg agtgatcccg ccgctgagtg tttcgtggcg cgcgtcggca aagccgttgg
     1621 cgcgcaacga cgccaccatc tccggccccg gtggtaggta ggcgacgctc ttgg
//
LOCUS       DAORNF010000771         1673 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_51706_length_1673_cov_1.495674, whole genome shotgun sequence.
ACCESSION   DAORNF010000771 DAORNF010000000
VERSION     DAORNF010000771.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51706_length_1673_cov_1.495674"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>352
                     /locus_tag="PLV68_21180"
     CDS             <1..>352
                     /locus_tag="PLV68_21180"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRE03823.1"
                     /translation="IGPLLRDEWGRRLTRDRAQQIIDTVARSCAITTRYTPHAFRHAW
                     TTLALDAGCSVRDIMAGGGWADASMIHYYDRSRSGVERNATHQLTDWLTNNSDATLHP
                     EDRDMLEWHRTLPPI"
     assembly_gap    353..457
                     /estimated_length=105
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <458..701
                     /locus_tag="PLV68_21185"
     CDS             <458..701
                     /locus_tag="PLV68_21185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03824.1"
                     /translation="PHRYPFLMVDRAEDYVDLARPGSRRWIRLAGRFAVLTAVWLAPT
                     TAFAVIYEHHEPLARGIARYFTDAIVDAVTPPTTTP"
     gene            complement(703..921)
                     /locus_tag="PLV68_21190"
     CDS             complement(703..921)
                     /locus_tag="PLV68_21190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03825.1"
                     /translation="MTDCVTADGRWTFRYDRGSAWVAGRRQTGGGRVVVELTRSEMDE
                     LAKAWDAVLARIGEHSTHVTGRVDGGPR"
     gene            complement(918..1400)
                     /locus_tag="PLV68_21195"
     CDS             complement(918..1400)
                     /locus_tag="PLV68_21195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03826.1"
                     /translation="MSEELMGWVVTVVATGPAGVDVDGAVEALLADGHDSVSSDASGG
                     VVSVIVADSNPTALKAATWVADGVLATLGDGWTLTGVQVQTEAAFDAELERPAIPPLV
                     GLAEAAGILGVSRQRVGQLVDRLLPVARLASGPVFLESAVLAFKAEPRRVGRPTSVAS
                     "
     assembly_gap    1441..1455
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          284 a          626 c          411 g          232 t
ORIGIN      
        1 catcggccca ctactacgcg acgaatgggg acgccgcctc acccgcgacc gcgcacagca
       61 gatcatcgac accgtcgccc gctcctgcgc catcaccacc cgctacaccc cgcacgcgtt
      121 ccgccacgcc tggaccaccc tcgcgctcga cgccggatgc tcagtgcgcg acatcatggc
      181 cggcggcggc tgggccgacg cctcgatgat ccactactac gaccgcagca gatccggcgt
      241 cgaacgcaac gccacccacc aactcaccga ctggctcacc aacaacagcg acgccacgct
      301 gcaccccgag gatcgggaca tgctcgaatg gcaccgcaca ctcccaccaa tcnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngcc gcaccgctat ccgttcctga
      481 tggtcgaccg cgccgaggac tacgtcgacc tcgcccgccc aggatcgcgg cgatggatca
      541 ggctggcggg ccggttcgcc gtgctgacgg cagtgtggct ggcgccaacc accgcgttcg
      601 cggtcatcta cgagcaccac gaaccgctcg cgcgtggcat cgctcgctac ttcaccgacg
      661 cgatcgtgga tgcggtcacc ccgcccacga caacgccctg actcaacgag gcccgccatc
      721 gacccggccg gtgacgtgcg tcgagtgctc accgatgcgc gccagcacgg cgtcccacgc
      781 cttggcgagc tcgtccatct ccgatctggt caactcgacc acgactcgac ccccaccggt
      841 ctgccgccga ccggccaccc aggccgagcc gcggtcatag cggaacgtcc agcggccgtc
      901 cgctgtcacg cagtcagtca cgacgccacc gacgtaggcc gaccaacccg acgcggctcc
      961 gccttgaacg ccagcactgc cgactccaag aacaccggac ccgaggccaa ccgcgcgacc
     1021 ggcagcaacc ggtctaccaa ctgaccaacg cgctgcctcg atacccccaa aatgcccgcc
     1081 gcctcagcca acccgaccag gggagggatc gctggccgct cgagctcagc atcgaacgcc
     1141 gcttcggtct gcacctgcac cccggtcaac gtccacccat ctccgagcgt cgcgagcaca
     1201 ccgtcggcga cccacgtcgc agccttcaac gcagtcgggt tcgagtccgc cacgatcacg
     1261 ctgacgacac cacctgaagc atcactcgac accgagtcat gaccatcggc caacagcgcc
     1321 tcgacagcgc cgtcgacgtc gaccccggcc ggaccagtgg ccaccaccgt gaccacccag
     1381 cccatcagtt cctcactcat acgattcctc cactcctgcg gatctgtgcc cgcacgttct
     1441 nnnnnnnnnn nnnnncggtg atccgacggt gtcgaagcga ccgtgacgat cggcatcgac
     1501 ttgtcggcgg ggaccaactt gacgtggccc ttgcccggtc ggacttccca accctgggct
     1561 gccacttggt cgatcagggc gttgatctct ttgttgttcg tgtgcttcgc catgatcttc
     1621 tcccgtccgg ctttacgttg tcaagtataa tcctcccgca ccacgtcgtc aac
//
LOCUS       DAORNF010000772         1670 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_51807_length_1670_cov_1.360372, whole genome shotgun sequence.
ACCESSION   DAORNF010000772 DAORNF010000000
VERSION     DAORNF010000772.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51807_length_1670_cov_1.360372"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            223..879
                     /locus_tag="PLV68_21200"
     CDS             223..879
                     /locus_tag="PLV68_21200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HRE03827.1"
                     /translation="MLIEDRRGVRINLTGAVDPALFVALHEAFTSAPTTAGFNPAPLP
                     GSAMAPPAAPMASAPLPAAAPPPGFAQPGFPHPGSVQSGFTHPGSAPPSGGDRTMTVA
                     ELRSMRSGAIPAVQLPDGRRMLIGSGLVFGRQPGPNPDLPAATLVPISDPSLSKTHAS
                     FGPTPTGVWVCDHQSTNGSTVEVGSSAAVCAPGARVEVPIGATVVLGEIRIPIVEWTQ
                     "
     assembly_gap    915..966
                     /estimated_length=52
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <967..>1392
                     /locus_tag="PLV68_21205"
     CDS             <967..>1392
                     /locus_tag="PLV68_21205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03828.1"
                     /translation="PGRLNPALTWIAAGLALGASLAPFYSGGDGLLMGDSTTAKTVCL
                     ALIIAPFVAAVTVGKSRPANGLAVGGGLAAATFFGMVATGVFKNANEYAKAGAGAYLA
                     AGAAVFGIVVVVDGLKSLGPARAKSGIAMLGIAGGVAVGI"
     assembly_gap    1393..1411
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1412..>1670
                     /locus_tag="PLV68_21210"
     CDS             <1412..>1670
                     /locus_tag="PLV68_21210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03829.1"
                     /translation="RYGGSWADFNLFSYDVVLGICSQLVGWTPAIGAVLGLLRGGRVG
                     SMVALGAAAPVTLFLLMPDDYYSGFGGLVYTYAHPLTSIGLV"
BASE COUNT          208 a          547 c          568 g          276 t
ORIGIN      
        1 tcggtctgtc ggcgcagctc gtgggtctcg cgcgtgtgca gctgccagtc gttggccgac
       61 agcagccgcg agagcgacgc gagttggagc acgccctcgt aggccttcgc ccgtcgcttc
      121 gccgcgtggt cgaccatggt gcagaagatc gagagcgacc cgtcgtcgtt gtccatcagc
      181 tcgatcagac gcgactgctg cggccagtcg acgtgcgagg cggtgttgat cgaggaccgc
      241 cgtggcgtgc ggatcaacct gaccggcgcg gtcgacccgg cgctgttcgt ggcgctgcac
      301 gaggcgttca cgtcggcgcc gaccaccgcc gggttcaatc cggccccact acccggttcg
      361 gccatggcgc caccggcggc gccgatggct tcggcgccct tgcccgccgc ggccccgcca
      421 cccggcttcg cccagcccgg ctttccccat cccggctccg tccagtctgg cttcacccat
      481 cccggctctg cgccaccatc cggtggcgac cggacgatga ccgtggccga gttgcggtcg
      541 atgcgcagcg gcgcgatacc ggcggtgcag ttgccggacg gcaggcggat gctgatcgga
      601 tcgggcctgg tgttcggtcg ccaacccggc cccaaccccg acctacccgc ggcgacgctg
      661 gttccgatca gcgatccgtc gctgtccaag acgcacgcgt cgttcgggcc gaccccgacc
      721 ggcgtctggg tctgcgacca ccaatcgacc aacggaagca ccgtcgaggt cggcagctct
      781 gccgcggtgt gcgcccctgg cgcccgcgtc gaggtaccga tcggagcaac ggtggtgctg
      841 ggcgagatcc gaatcccgat cgtggagtgg acacaatgag cgacccctac tactccggtg
      901 gctacgccgg cgggnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      961 nnnnnncccg gccgcctcaa cccagccctg acctggatcg ccgcgggcct cgcgctcggt
     1021 gccagcctcg cgccgttcta ctccggcggt gacgggctgc tgatgggtga ctcgaccacg
     1081 gccaagacgg tgtgcctggc gttgatcatc gcccctttcg tcgcggcggt cacggttggc
     1141 aagtcgcggc ccgccaacgg attggcggtc ggtggcggtc tggcggcggc caccttcttc
     1201 ggcatggtgg cgaccggggt gttcaagaac gccaacgagt acgccaaggc aggagcgggc
     1261 gcgtacctcg ccgcgggagc ggcagtattc ggcatcgtgg tggtcgtcga cgggctgaag
     1321 tcgctcggtc cggcacgggc caagagcggg atcgcgatgc tcggtatcgc cggtggggtc
     1381 gcggtcggga tcnnnnnnnn nnnnnnnnnn natcgctacg gcgggagttg ggccgacttc
     1441 aacctgttca gctacgacgt cgtgctcggg atctgctcgc agttggtggg ctggaccccg
     1501 gcgatcggag cggtgctcgg cctgctgcgt ggcggcaggg tgggcagcat ggtggccttg
     1561 ggtgcggcgg ctcccgtcac gctgttcctg ctgatgccgg acgactacta cagcgggttt
     1621 ggtggattgg tgtacacgta cgcccatccc ttgacctcca tcggcctggt
//
LOCUS       DAORNF010000773         1667 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_51922_length_1667_cov_1.792184, whole genome shotgun sequence.
ACCESSION   DAORNF010000773 DAORNF010000000
VERSION     DAORNF010000773.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51922_length_1667_cov_1.792184"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1058)
                     /locus_tag="PLV68_21215"
     CDS             complement(<1..1058)
                     /locus_tag="PLV68_21215"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRE03830.1"
                     /translation="MEFEPSEEMSVRLALIDVMRHDVIPLEGEMLFGAPETLAAKIAV
                     VQGKVREMGLWAPNFPLEFGGLGLSMVDHGLFSEALGRSPLGHVAFGTQAPDAGNIEI
                     LHLFGTPEQKQRWLKPLVAGEIRSCFSMTEPETAGSNPTMLATRAVRDGDEWVLDGQK
                     WFTSSADGAAFAIVMAVTDPDAESHRRASMFIVPTDTPGFELVRNVSVMGHTGHGHAS
                     HAEVSYQSCRVPAANLLGGLGRGFAIAQERLGPGRIHHCMRWLGIADRALEMLCDRAN
                     SRQMTPTEKLADKQVVQHWVAELATEIRAARLLTLHAAWRIDQVGAPAARDEIAGIKF
                     HVAGVMLNVRLAPAPSTR"
     gene            complement(1147..>1667)
                     /locus_tag="PLV68_21220"
     CDS             complement(1147..>1667)
                     /locus_tag="PLV68_21220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03831.1"
                     /translation="LDHPTADWGELVAAAAGHGGWDQWRSGGLAAAAHPEVDAAPEAT
                     RDLLAELAAELVARRTITTEVWEGDDRQALVDSPHWRAGQRRREIIFAIERAMLAAEQ
                     CDPAGLRSAAASIAARDTDVRYPDLAESLLACAGEAETAKTISAASLDRLESALVTTP
                     FAAAVRLLVHPE"
BASE COUNT          248 a          563 c          602 g          254 t
ORIGIN      
        1 ctgcgcgtgg acggagccgg tgccaatcgc acgttgagca tcaccccggc gacgtggaac
       61 ttgatcccgg cgatctcgtc acgcgccgcg ggcgcgccga cctgatcgat ccgccacgcg
      121 gcatgcagcg tcaggagccg cgcggcgcga atctccgttg ccaactcggc gacccagtgc
      181 tgcacgacct gcttgtccgc aagcttctcg gtcggggtca tctggcggct gttggcgcgg
      241 tcgcacagca tctccagggc gcggtcggcg atgccgagcc agcgcatgca gtggtggatg
      301 cggcccggcc ccaggcgttc ctgggcgatc gcgaagccgc ggcccaggcc gccgagcaag
      361 ttggccgccg ggacgcggca cgattggtac gaaacctccg catggctggc gtgaccgtgg
      421 cccgtatggc ccatcacgct gacgttgcgc accaactcga agccgggggt atcggtcggc
      481 acgatgaaca tgctcgctcg gcgatgcgac tcggcgtcgg gatcggtcac ggccatcacg
      541 atcgcgaacg ccgcgccgtc ggccgacgac gtgaaccact tctggccgtc gagcacccac
      601 tcgtcaccgt cgcgcaccgc ccgcgtggcg agcatcgtcg ggttcgatcc ggccgtctcc
      661 ggttcggtca tcgagaagca cgagcggatc tcgccggcga cgaggggctt gagccagcgt
      721 tgcttctgct cgggggtgcc gaacaggtgg agaatctcga tgttgccggc gtcgggagct
      781 tgcgtgccga acgcgacgtg accgagcggc gagcgcccga gtgcttcgga gaacaacccg
      841 tggtcgacca tcgacaggcc gagaccaccg aattcgagcg ggaagttcgg cgcccacagt
      901 cccatctcgc gcaccttgcc ctgtacgacg gcgatcttgg cggcgagggt ttccggagca
      961 ccgaacaaca tctcgccttc cagcgggatc acgtcgtggc gcatgacgtc gatcagcgcc
     1021 agtcggaccg acatctcctc ggacggttcg aactccatgg ctcctccttg ctgcgctcgt
     1081 cgaccctgcg gcagagcgcg taaagccagc gtgcggccat cgcgccgcgc aagccagggg
     1141 cgaaggtcac tcggggtgca ccaggaggcg gacggcggcg gcgaagggag tggtgaccaa
     1201 ggccgattcg aggcggtcga gggaggctgc actgatcgtc ttggctgtct ctgcttcgcc
     1261 ggcgcaggcg agcagtgact cggcgaggtc ggggtagcgg acgtcggtgt cgcgggcggc
     1321 gatgctggct gcggcagagc gcagcccggc cgggtcgcac tgctcggcgg ccagcatcgc
     1381 ccgctcgatc gcgaagatga tctccctccg ccgctggccg gcgcgccagt gcggcgagtc
     1441 gaccagcgcc tggcgatcgt cgccctccca cacctcggtg gtgatggtgc gccgggcgac
     1501 gagctcggcg gcaagttcgg ccaagagatc gcgagttgct tcgggtgcgg cgtcaacttc
     1561 ggggtgcgcg gccgcggcca atccgcccga gcgccactgg tcccagccgc cgtgcccggc
     1621 ggcggcagcg acgagttcgc cccagtcggc ggtggggtgg tcgaggc
//
LOCUS       DAORNF010000774         1667 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_51926_length_1667_cov_1.686104, whole genome shotgun sequence.
ACCESSION   DAORNF010000774 DAORNF010000000
VERSION     DAORNF010000774.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51926_length_1667_cov_1.686104"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="PLV68_21225"
     CDS             <1..485
                     /locus_tag="PLV68_21225"
                     /inference="COORDINATES: protein motif:HMM:NF028352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotide-binding domain containing protein"
                     /protein_id="HRE03832.1"
                     /translation="DPPVRWLVIDAQAIHDMDVTAAQRFAEPSAPIGLARPGCQRVII
                     VAGSQHEVTKRQLAALDRTVTVLRIDPRDQSFLDQAKVTIEAADGLILTGGHTARSVL
                     STGARRLDIGGEVETGIPWAVTTANGRDLAVITKAGGFGDDGALMRAVRFLTTAQSAT
                     "
     gene            482..>1163
                     /locus_tag="PLV68_21230"
     CDS             482..>1163
                     /locus_tag="PLV68_21230"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:NF016084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HRE03833.1"
                     /translation="MNTTPIVAITMGDPFGVGPEVVVKALARDDVRSLCRPVVIGDVA
                     RLQRAAELCGVTVQIENVSDGPLTPAAVAVWPGRIPVIEVGRVPADLAWGQVSPAGGD
                     AAYRYVERAATLAMAGDVQAICTAPLNKEALHAAGHIYPGHTELLAHLTGTDEVSMML
                     EAPGLRVVHVTTHIGLIDAIARIEPGLVERTIRRADETLRRAGIVAPRFAVCGINPHA
                     GEQGLFGHG"
     assembly_gap    1164..1373
                     /estimated_length=210
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          233 a          522 c          491 g          211 t
ORIGIN      
        1 ccgacccgcc ggtcaggtgg ctggtgatcg atgcccaggc gattcacgac atggatgtca
       61 cggcggcgca gcgtttcgcc gagccatccg cgccgatcgg cctggcccga ccgggctgcc
      121 agcgggtcat catcgtcgcc ggatcgcagc acgaggtgac caagcgtcaa ctggctgcgc
      181 tcgaccgcac cgtcacggtg ttgcggatcg acccgcgcga ccagagcttc ctcgatcagg
      241 ccaaggtcac catcgaagcc gccgacgggc tgatcctcac cggcgggcac accgcccgat
      301 cggtcctcag caccggggcc aggcgactcg acatcggcgg cgaggtcgag accggcatcc
      361 cgtgggcggt cacgacagcc aacggccgtg acctcgcggt gatcaccaag gcggggggct
      421 tcggcgacga cggcgcgctg atgcgtgcgg tgcggttcct caccacggca caatcggcga
      481 cgtgaacacc acacccatcg tcgcgatcac catgggcgac ccgttcgggg tcgggcccga
      541 agtcgtcgtc aaggcgctgg cgcgcgacga tgtccgctcc ctctgccggc ccgtggtcat
      601 cggtgatgtc gcccgcctgc aacgcgccgc cgagttgtgc ggcgtgacgg tccagatcga
      661 gaacgtgtcc gacggccccc tcactcccgc ggcggtcgcc gtctggccag gccggatccc
      721 cgtgatcgaa gtcggccggg ttccggccga tctcgcgtgg ggacaggtgt cgcccgcggg
      781 cggcgatgcg gcctaccgct acgtcgaacg ggcagcgacg ctggcgatgg ctggcgacgt
      841 ccaggccatc tgcaccgccc cgctgaacaa ggaagccctc cacgccgctg gccacatcta
      901 ccccgggcac accgaactac tcgcccatct gaccggcacc gacgaggtct cgatgatgct
      961 cgaagcgcca ggcctgcgcg tggtgcacgt caccacccac atcggtctga tcgacgcgat
     1021 cgcgcggatc gaaccgggtc tggtcgagcg caccatccgg cgtgctgacg agacgttgcg
     1081 ccgggccggc atcgttgctc cgcgcttcgc agtgtgcggc atcaaccccc acgccggcga
     1141 gcagggtctc ttcggccatg gcgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngcgcacc
     1381 tcggtcgacc acggcaccgc gttcgacatc gccggcacgg gcgtggccga cgagcaatcg
     1441 atggtcgaag cgctgcgcca ggcggtacgc ctcgcgacgc gctgaggcgg acgatcgccg
     1501 agacggtcag catcagcacc atgtatcggg tcccgaacgg tacggacctg gtcggtactg
     1561 tggacccatg accatctcac ccgacgacaa gaactggacg tgggtactcg accgggcgtg
     1621 tcccgagtgc gggttcgacg ccagctcctt cgatccggag acgacgg
//
LOCUS       DAORNF010000775         1667 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_51928_length_1667_cov_1.669975, whole genome shotgun sequence.
ACCESSION   DAORNF010000775 DAORNF010000000
VERSION     DAORNF010000775.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51928_length_1667_cov_1.669975"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1519
                     /locus_tag="PLV68_21235"
     CDS             <1..1519
                     /locus_tag="PLV68_21235"
                     /inference="COORDINATES: protein motif:HMM:NF037016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HRE03834.1"
                     /translation="FDAIFVPKGYDQTLLRDLGLTRDIELAFVGSLNDQSWSCSFQDC
                     DTPAGNVIIGLQSPFGGTNLTIPVTPGAIKPVADFREPVPGPSIDGWLPATTYSYEAA
                     FDGNFNTGLGEFGSFEITPRPCQTTITQTVHQTTPGGAVTFSARVSTSVAPVSGYVVF
                     YQGPPSDGLTLTGVIPVPAGNVVSSGPITALPSGATTTVTALFTSTNGNQATVQHIVA
                     SEPPPPTANDDFVTTPRGQAVTFAPFANDVSPTGDPLHIEELTGSGPGDLDFDDATGN
                     YTYTPPPEFVGDASFQYRAGDDSGGISPQASITIIVTCAPTAVTDLYATDFEVAITVD
                     LASNVLANDDTCDEAVTLLGQPDQGTVTDFDPFNGTFTYTPPAGFTGIATFTYAYTSG
                     VVIDDVGTVLIAVGPAADDPDSTSTTANTAATTTTTTDGAATTTSPATSTTIDSGGAS
                     PTTTSGPDNPTPPTAPGDGVLPDTGSSPNTVLLLAGALLVAGALLRVRPARRTRS"
BASE COUNT          310 a          619 c          484 g          254 t
ORIGIN      
        1 attcgacgcc atcttcgtcc ccaagggcta tgaccagact ttgctgcgcg atctcggatt
       61 gacgcgggac atcgaactgg ccttcgttgg cagcctgaac gaccagagtt ggtcgtgctc
      121 gttccaggac tgcgacacac cggccggcaa cgtgatcatc gggctccaat ccccgttcgg
      181 cggcacgaac ctgacgattc cggtcacgcc cggtgccatc aagcccgtcg ccgacttccg
      241 cgagcccgta cccgggccgt cgatcgacgg atggttgccg gcgacgacgt acagctacga
      301 ggcagcgttc gacggcaact tcaacacggg gttgggcgag ttcggatcgt tcgagatcac
      361 acctcggccg tgccagacca ccatcaccca aacggtgcac caaaccacac cgggcggtgc
      421 ggtgaccttc tcggcccggg tcagtacatc ggtcgctccc gtctccggct acgtcgtctt
      481 ctaccaaggc ccgcccagtg acggactgac gctcacgggc gtcatccccg tgcccgcggg
      541 caacgtcgtc agcagcggac cgatcaccgc actcccctcg ggtgcgacga cgacggtgac
      601 agccctgttc acgtccacca acggcaacca ggccacggtg cagcacatcg tcgccagcga
      661 gccgccgccg ccgactgcca acgacgactt cgtgacgaca ccgcggggtc aagcggtcac
      721 cttcgctccg ttcgccaacg acgtcagccc gaccggtgat ccgctgcaca tcgaggaact
      781 gaccggcagc ggtcctggcg acctcgactt cgacgacgcc acgggcaact acacctacac
      841 gccgccgccc gagttcgtgg gcgatgcctc gttccagtac cgcgccggcg acgactcggg
      901 cggcatctcg ccacaagcat cgatcaccat catcgtcacc tgcgctccca ctgctgtcac
      961 cgacttgtac gccacggact tcgaggtcgc gatcaccgtc gatctcgcca gcaacgtgct
     1021 cgccaacgac gacacgtgcg acgaagccgt gaccctgctc ggtcagcccg accagggaac
     1081 cgtcaccgac ttcgatccct tcaacggaac cttcacctac acacccccgg cgggtttcac
     1141 cggtatcgcg acgttcacct acgcctatac gtccggagtg gtgatcgacg acgtcggcac
     1201 cgtgttgatc gccgtcgggc ccgcggccga cgaccccgac agcaccagta ccaccgccaa
     1261 caccgcggcg acgaccacca cgaccaccga cggcgcggcc accacgacaa gccctgcgac
     1321 cagcaccacg atcgactccg gcggtgccag ccccacgacc acgtcagggc cagacaatcc
     1381 gacaccgccg accgcgccgg gcgacggtgt actgcctgac accggttcat cgccgaacac
     1441 cgtgttgttg ctggccggag ccctgctcgt ggccggagcg ttgctccgag tcaggccggc
     1501 acgtcggacc cggagctgat cgccgtcacg gggcggtgga ggccatggcg gagcaacgaa
     1561 cctgcaccac ctcgcgcaaa cgttccgatc aacaagtaca gcgcgacgcc aggtggtgcg
     1621 tgtcaggtgg cgatgagcga ttcgctgtcg agatcgatca cgtgttg
//
LOCUS       DAORNF010000776         1666 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_51990_length_1666_cov_1.320919, whole genome shotgun sequence.
ACCESSION   DAORNF010000776 DAORNF010000000
VERSION     DAORNF010000776.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1666
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51990_length_1666_cov_1.320919"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>881)
                     /locus_tag="PLV68_21240"
     CDS             complement(<1..>881)
                     /locus_tag="PLV68_21240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRE03835.1"
                     /translation="EKQLQVSTLIRVYRVRGHLIADLDPLHWKHPVMPKELDSATYGL
                     TIWDLDREFMTGGVGGKAKQTLGSLLDTLLDAYCRTIGIEYMHIQDTDEQRWIQSQVE
                     GVTTTFTKEEKHRILERLNAAEAFEKFLATKYVGVKRFGLEGAESAIPILDSLLSDAV
                     DAGLDSAVLGMAHRGRLNVLANLMGKSYEKIFRDFEGHVDPTSVQGSGDVKYHLGAMG
                     KYESPSGGDIKVELAAHPSHLETVDPIVLGMVRAIQDRIEPPNSFPSLPILIHGDAAF
                     AGQGVVAECLAMSDING"
     assembly_gap    882..978
                     /estimated_length=97
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<979..>1275)
                     /locus_tag="PLV68_21245"
     CDS             complement(<979..>1275)
                     /locus_tag="PLV68_21245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HRE03836.1"
                     /translation="KVRNNKLTVADFQGATVSLTNPGTIGTVQSVPRLMPGQGVIVGV
                     GNIDYPAEFQGTDERTLGAIGVSKVVTVTSTYDHRIIQGAESGMFLKYVHELLL"
     assembly_gap    1276..1361
                     /estimated_length=86
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1362..1626)
                     /locus_tag="PLV68_21250"
     CDS             complement(<1362..1626)
                     /locus_tag="PLV68_21250"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HRE03837.1"
                     /translation="MERSLAVPTATSFRNIPAKLLEVNRKMINGYRTRAGLSKTSFTH
                     IIGYAIARAIADAVPQMNNSYVEGPDGKPRIIRNQSVDIGLAVD"
BASE COUNT          225 a          458 c          516 g          284 t
ORIGIN      
        1 tagccgttga tgtcgctcat cgccaagcac tcggcgacca caccctggcc ggcgaaggcc
       61 gcgtcgccgt gaatcaggat cggcaacgac gggaacgagt tcggcggctc gatgcggtcc
      121 tggatggcac gcaccatgcc gagcacgatc gggtcgaccg tctcgaggtg gctggggtgg
      181 gccgccagct cgaccttgat gtcgccaccg ctcgggctct cgtacttgcc catcgcaccg
      241 aggtggtact tgacgtcgcc ggaaccctgc accgacgtgg gatcgacatg gccttcgaag
      301 tcgcggaaga tcttctcgta gctcttgccc atcaggttgg ccaacacgtt gaggcggccg
      361 cggtgggcca tgcccagcac cgccgagtcg agcccggcgt cgaccgcgtc gctgagcaac
      421 gaatcgagga tcgggatcgc cgactcggca ccttcgagac cgaaccgctt gacgcccacg
      481 tacttggtgg cgaggaactt ctcgaacgcc tcagccgcgt tgagccgttc gaggatgcgg
      541 tgcttctctt ccttggtgaa ggtggtggtc accccttcga cctggctctg gatccaacgc
      601 tgctcgtcgg tgtcctggat gtgcatgtac tcgatgccga tcgtgcggca gtaggcgtcg
      661 agcagcgtgt cgagcaacga gccgagggtc tgcttggcct tgccgcccac gccgccggtc
      721 atgaactcgc gatcgaggtc ccagatggtg agcccgtagg tggccgagtc gagctccttg
      781 ggcatcaccg ggtgcttcca gtgcagcggg tcgaggtcgg cgatgaggtg accgcggacg
      841 cggtagacgc ggatcagcgt cgagacctgg agctgctttt cnnnnnnnnn nnnnnnnnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      961 nnnnnnnnnn nnnnnnnncg agcagcagct cgtgcacgta cttgaggaac atgccgctct
     1021 cggcaccctg gatgatgcgg tggtcgtaag tgctggtgac cgtgaccacc ttgctgacgc
     1081 cgatcgcgcc gagggtgcgc tcgtcggtgc cctggaactc ggccgggtag tcgatgttgc
     1141 cgacgccgac gatcacgcct tgcccgggca tcaaccgggg cacgctctgc acggtgccga
     1201 tcgtgccggg gttggtgagc gacacggtcg caccctggaa gtcggcgacc gtgagcttgt
     1261 tgttgcggac cttgcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncgtcgaccg ccaggccgat
     1381 gtcgaccgac tggttgcgga tgatgcgcgg cttgccgtcg gggccctcga cgtagctgtt
     1441 gttcatctgc ggtaccgcgt cggcgatggc gcgggcgatg gcatagccga tgatgtgggt
     1501 gaagctggtc ttgctgagcc cggcgcgagt gcggtacccg ttgatcatct tgcggttgac
     1561 ttccagcagc ttggccggga tgttgcggaa cgacgtggcg gtcggcaccg ccaggctgcg
     1621 ctccatgttg gcgacgatgg ccgcaccagc gccgcggatg atctca
//
LOCUS       DAORNF010000777         1665 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_52017_length_1665_cov_2.173292, whole genome shotgun sequence.
ACCESSION   DAORNF010000777 DAORNF010000000
VERSION     DAORNF010000777.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52017_length_1665_cov_2.173292"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    240..274
                     /estimated_length=35
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            420..803
                     /locus_tag="PLV68_21255"
     CDS             420..803
                     /locus_tag="PLV68_21255"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRE03838.1"
                     /translation="MLLDTHAFVWALTTPDRLSDAARSAVSDRAHNLHVSAASVWEMA
                     IKVRSGKWPDAEPIVAGVEALARRLGAIMVGIDGTDARCAGLLDWGHRDPFDRMLAAQ
                     ATNRQFPLITRDTQFRNVTGLAVVW"
     gene            complement(939..1367)
                     /locus_tag="PLV68_21260"
     CDS             complement(939..1367)
                     /locus_tag="PLV68_21260"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HRE03839.1"
                     /translation="MELADLPQIASALSPWAHLATVRADGTPDVVPVHPAWLDGDLWV
                     MSGATARKVRNVADNAAVALHWQVTEAGDGLEVWGEASVHTDRDTKHRLWTGVFDYDL
                     NLFAPDGPDGSGTAFVRVRPTSALYLRAYGMGGTERWSAG"
     gene            1452..>1665
                     /locus_tag="PLV68_21265"
     CDS             1452..>1665
                     /locus_tag="PLV68_21265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439568.1"
                     /note="negatively supercoils closed circular
                     double-stranded DNA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HRE03840.1"
                     /translation="MAADYTAAQIQTLSGLGHVRLRPEMYLGATDVAALHHLIWEIVD
                     NSVDEVMAGYGDRVIVTLNADGSVSVQ"
BASE COUNT          278 a          577 c          512 g          263 t
ORIGIN      
        1 ggaaaccccg catccgcggc agcccgatcg cccaccccgg cacggacaac gtcggcaccc
       61 gcgcaacacc catcgccccg gcgaaacggc aaccttctgc cttacgaatg acacgacccg
      121 acggccgtga cgccacgacg gggttggtca tctagacttg gtcactgtga ccatcacgat
      181 gaatgtggcc gaggcgaagg caaagctctc cgagctgctc gacgcggctg cgcgcggtgn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnatcgcg acgttgaccc cgatcgcacc
      301 cctcgctgaa cgcgagctcg gtttccttac gctcaccgtt gacgacgcgt tctttgcccc
      361 cctcggcgac gacgagctcg ctgagtggga atgagacgag acgaaccacg atgacggact
      421 tgttgctcga cacccacgcc ttcgtctggg cattgaccac accggatcgc ctcagcgatg
      481 cggcccgatc agccgtcagc gatcgagctc acaacctgca cgtctccgcc gccagcgtgt
      541 gggagatggc gatcaaggtg cgatcgggca agtggcccga tgccgagccg atcgttgccg
      601 gggtcgaagc actcgcccgc cgacttggcg caatcatggt cggcatcgac ggcaccgacg
      661 cccgctgcgc cggtctgctc gattggggcc accgcgaccc cttcgaccga atgctcgcgg
      721 cccaggccac caaccgtcag ttcccgctga tcacccgcga cacccagttc cgcaacgtca
      781 ccggcctcgc cgtcgtctgg taaccctccg ttcgcaacgc cgcggcgtgt ccctagcggc
      841 gaatccggcg tccgcggatc gtggatgtac cggtttccgg gcaatcggta ctccgcggag
      901 gctggatcta ccggcagttg cctctggtcg cgcccgactc agccggccga ccagcgctcg
      961 gtgccgccca tgccgtaagc gcgcagatac agggcgctgg tggggcggac gcgcacgaac
     1021 gcggtgccgc tgccgtcggg tccatcgggc gcgaacaggt tgaggtcgta gtcgaacacg
     1081 ccggtccaca gccggtgctt ggtgtcgcga tcggtgtgca cgctggcttc accccacacc
     1141 tccaagccgt cgcccgcctc ggtgacctgc cagtgcaacg cgaccgcggc gttgtcggcg
     1201 acatttctaa ccttgcgcgc cgtggcgccg ctcatcaccc acaggtcacc gtcgagccag
     1261 gccggatgca ccggcaccac atcgggcgta ccgtcggcgc ggacagtggc caggtgcgcc
     1321 cacggcgaca gggcggatgc gatctggggc aggtctgcga gttccatctg cggactgtac
     1381 cctctgggtg accaagaaca catgttcgat ccatgaccgt gtgagacccg tcagacagac
     1441 tggacgacgc tatggccgcc gactacaccg ctgcacagat ccagaccttg tccgggttgg
     1501 gtcacgtccg actccggccc gagatgtacc tgggggccac cgatgtcgcc gcgctgcatc
     1561 atctgatctg ggagatcgtc gacaactcgg tcgacgaggt gatggccggg tacggcgacc
     1621 gggtcatcgt caccctcaac gccgacggat cggtcagcgt gcagg
//
LOCUS       DAORNF010000778         1662 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_52152_length_1662_cov_1.240199, whole genome shotgun sequence.
ACCESSION   DAORNF010000778 DAORNF010000000
VERSION     DAORNF010000778.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1662
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52152_length_1662_cov_1.240199"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>231)
                     /locus_tag="PLV68_21270"
     CDS             complement(<1..>231)
                     /locus_tag="PLV68_21270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03841.1"
                     /translation="AEYEPAEAQKILLGFRRDFQDVYFTNYVNYDRVSLGYDAIFARK
                     VGLGLSSAYRYERYKGEVSRNDLPAALDVIPVS"
     assembly_gap    232..470
                     /estimated_length=239
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<471..712)
                     /locus_tag="PLV68_21275"
     CDS             complement(<471..712)
                     /locus_tag="PLV68_21275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03842.1"
                     /translation="MQVDQWNVPEEFKSAASDRDLDLGLTSGLTGPGLNSRFGYGPAV
                     DARWRFFPKTAVVLDFRHEWFRWQDNVVDAQGDGISS"
     assembly_gap    733..843
                     /estimated_length=111
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<844..1251)
                     /gene="eutB"
                     /locus_tag="PLV68_21280"
     CDS             complement(<844..1251)
                     /gene="eutB"
                     /locus_tag="PLV68_21280"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF018461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008851 - ethanolamine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HRE03843.1"
                     /translation="MSARLQTRFTIGLACLLSGTAVAAPGDHLGDETTQLVPYLEAGI
                     FHRTNAFLVEGPAGGGAEPVAGTAFVIRPGIGLRAKGSGFELNLDFDYEAKKYFQAEV
                     TNLDRFNTIGLKGRMSTRLQPNHPFDDVRGITAS"
     gene            complement(1297..>1662)
                     /locus_tag="PLV68_21285"
     CDS             complement(1297..>1662)
                     /locus_tag="PLV68_21285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03844.1"
                     /translation="QLDEAEREGDAETVECIRLRKSKISALLAVSSLAQKDMNLALSS
                     GQDARAEHEFRKITVAQAKVRQFQSEVQACTGEGSVADSSSSVDAESNLDEDDDTQGL
                     GISDGVVGVDPPGTTPFEY"
BASE COUNT          216 a          414 c          453 g          229 t
ORIGIN      
        1 gagacgggga tgacgtcgag cgccgcggga aggtcgttgc gcgagacctc gcccttgtag
       61 cgctcgtagc ggtaggcgga gctgagtccc aggcccacct tgcgggcgaa gatcgcgtcg
      121 tacccaaggc tgacccggtc gtagttcacg tagttcgtga agtagacgtc ctggaagtcg
      181 cggcggaatc ccaggaggat cttctgcgct tccgcgggct cgtactcggc gnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gagctgatgc
      481 cgtcgccctg cgcatccacc acgttgtcct gccagcggaa ccactcgtga cggaagtcga
      541 gcaccaccgc ggtcttgggg aagaagcgcc agcgggcgtc gacggccggg ccgtagccaa
      601 agcggctgtt gaggcccgga cccgtcaggc cgctggtcag gccgaggtcc agatcgcggt
      661 ccgaagccgc cgacttgaac tcctcgggga cgttccactg gtcgacctgc acccggaagc
      721 ccgcgtcgac ctnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      841 nnncgaggcg gtgatgcccc tgacgtcgtc gaagggatgg ttgggttgca gacgcgtgct
      901 catccgcccc ttcaggccga tcgtgttgaa gcggtcgagg ttcgtgacct cggcctggaa
      961 gtacttcttg gcctcgtagt cgaagtcgag gttcagctcg aagccggagc ccttggcgcg
     1021 cagtccgatg cccggtcgga tcacgaaggc cgttccggca acgggttcgg cgccgccgcc
     1081 agcaggtcct tccaccagga aggcgttggt gcgatggaag atgcccgcct cgaggtacgg
     1141 gaccagctgg gtggtctcgt caccgagatg atcgcctggc gcggccaccg ccgtgcccga
     1201 gaggaggcag gcgagtccga tggtaaagcg tgtttggagg cgagcagaca tcagcaaccg
     1261 tgggaatgag gcaaccgcag cttcggcgaa gaccgctcag tactcgaagg gggtggtgcc
     1321 cgggggatcg acgccgacga caccgtccga gatcccgaga ccctgcgtgt cgtcgtcttc
     1381 atcgaggttc gactcggcgt cgaccgacga gctggagtcc gcgacacttc cttctccggt
     1441 gcaggcttgt acttcggact ggaactgccg aactttcgcc tgagccaccg tgatcttgcg
     1501 gaattcatgc tcggcgcgag cgtcctgccc cgacgagagc gccaggttca tgtccttctg
     1561 cgccagcgac gagacggcca gcagggcgga gatcttgctc ttgcgcaggc gaatgcactc
     1621 gacggtctcg gcgtcgccct cccgctcggc ctcgtcgagc tg
//
LOCUS       DAORNF010000779         1657 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_52333_length_1657_cov_1.784644, whole genome shotgun sequence.
ACCESSION   DAORNF010000779 DAORNF010000000
VERSION     DAORNF010000779.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1657
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52333_length_1657_cov_1.784644"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="PLV68_21290"
     CDS             <1..226
                     /locus_tag="PLV68_21290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HRE03845.1"
                     /translation="IPAADELDAAALDQVLTSALVECESAGITGAAITPFVLGRIGEA
                     TAGRSVPANLALAENNARVAAAVATAVAAS"
     gene            285..872
                     /locus_tag="PLV68_21295"
     CDS             285..872
                     /locus_tag="PLV68_21295"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HRE03846.1"
                     /translation="MRALVVVCHPKPDSFTHAMAAAATEGLERAGHSVEVIDLYALGF
                     QPAMSEQERRAYHSDEPIVDPLVASSAEAVHQAEMLVFVYPTWWSSLPAMLKGWLEKT
                     MVPGVAFVFNQAGKVRPGMTHIRRVVGISCYGSPRTYVRLVNDNGRRTLLRALRLNTG
                     PRCRTTWLACYSLDTSTALDRAGFLHRITSTLAAL"
     gene            869..1453
                     /locus_tag="PLV68_21300"
     CDS             869..1453
                     /locus_tag="PLV68_21300"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HRE03847.1"
                     /translation="MKTLLVYCHPNDQSFVAAARDAALAGLDAGGHEVRVRDLYAERF
                     DPVFSADDRTNHRLPLSVKPHIAGHAADLAWCEQLVLVYPTWWAGQPAMLKGWIDRVW
                     VNEVAWTLPAGSNRIRRGLTNIRRITVVTSHGSSKLINALEGEGGKRVVSRSLRLMCN
                     RWCRLRWLALYGIDTAAPAARDAFLAKVRTRLAG"
BASE COUNT          235 a          576 c          575 g          271 t
ORIGIN      
        1 gattcccgct gccgacgaac tcgacgcggc cgcgctcgac caggtgttga cgtcggcgct
       61 tgtggagtgc gagtcggcgg gcatcaccgg cgcggcgatc accccgttcg tgctcggccg
      121 catcggcgaa gccacggccg gtcgtagcgt tcccgcgaac ctcgcgttgg ccgagaacaa
      181 cgcccgagtt gccgccgccg tcgcgaccgc cgtcgccgcg tcctgaccgt tctcgacgtc
      241 cgcactggcc tccggcgggt gcccacgacg gctacggttc ggtcatgcgc gcgctggtgg
      301 tggtctgtca cccgaagcca gacagcttca cgcatgcgat ggctgccgcg gccaccgagg
      361 gcctcgaacg cgccggtcac agcgtcgagg tgatcgatct gtacgcgctg ggtttccaac
      421 cggcgatgag cgagcaggag cgccgcgcgt atcactccga cgaaccgatc gtcgacccat
      481 tggtggcgtc gtcggccgag gcggtccatc aggccgagat gctggtgttc gtctacccga
      541 cctggtggtc gagcctgcct gcgatgttga agggctggct ggagaagacg atggtgccag
      601 gtgtcgcgtt cgtgttcaac caggcgggca aggtccgtcc cggcatgacg cacatccggc
      661 gggtcgtcgg catcagctgc tacgggtcgc cgcgtaccta cgtgaggctg gtcaacgaca
      721 acggacggcg caccctgctg cgcgccctgc gactcaacac cggcccgcgt tgtcgcacca
      781 cgtggctggc gtgctactcg ctcgacacgt cgacggcgct cgatcgggcc ggtttcctgc
      841 accgcatcac ctcgacgttg gcggcgctgt gaagaccctg ctggtgtact gccatcccaa
      901 cgaccagtcg ttcgttgccg ccgcgcgcga tgcagcgctg gcgggcttgg acgcaggtgg
      961 ccacgaggtg cgggtgcgcg atctgtacgc cgagcgcttc gacccggtgt tcagcgcgga
     1021 cgatcgcacc aaccaccggc tcccgctgtc cgtcaagccg cacatcgccg gccatgccgc
     1081 cgacctggcc tggtgcgagc agctcgtgct ggtctacccc acgtggtggg ctggccaacc
     1141 ggcgatgctc aagggctgga tcgaccgggt gtgggtcaac gaggtcgcct ggacgcttcc
     1201 tgccggctcc aaccgcatcc gccgtgggct gaccaacatc cgccgcatca ccgtcgtcac
     1261 cagccacggt tcgtcgaagt tgatcaacgc gctggaaggg gagggcggca agcgggtggt
     1321 cagccgcagc ttgcggctga tgtgcaaccg gtggtgccgg ctgcgctggc tcgcgctgta
     1381 cggcatcgac accgccgcac ccgccgcgcg cgatgcgttc ctcgccaagg tccgcacccg
     1441 cctcgccggc tgagcacccc tgccaccgac aacttgggaa ggagcagtgg agcggtgatc
     1501 ggcttgcccg acgacccggc gcggtcagcg gccaccggtg acgtcgagca tggtgccggt
     1561 ggtgtagctg gcttcgtcgc cggccagcca catcaccgca gcggcgactt cggcggtggt
     1621 gccgctgcgc cgcatcggga tgctggggcg cagccgg
//
LOCUS       DAORNF010000780         1654 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_52425_length_1654_cov_1.999375, whole genome shotgun sequence.
ACCESSION   DAORNF010000780 DAORNF010000000
VERSION     DAORNF010000780.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52425_length_1654_cov_1.999375"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="PLV68_21305"
     CDS             <1..606
                     /locus_tag="PLV68_21305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HRE03848.1"
                     /translation="DRYAAVIEAYIAGLEQYAADPDADLSRVASVASFFISRVDTEID
                     KRLEALGTPEALALRGKGAVAQGKLAYAMFDAAFSTERWRALASRGARVQRPLWASTS
                     TKNPAYPDTLYVDELIGPHTVNTLPEPTLEAFADHGTLARRVDADLDEATRAWQQLAA
                     VGIDLDDVAAVLEREGVASFQQSFDELLAALATKADELRNG"
     gene            876..>1271
                     /locus_tag="PLV68_21310"
     CDS             876..>1271
                     /locus_tag="PLV68_21310"
                     /inference="COORDINATES: protein motif:HMM:NF037785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rossman fold protein, TIGR00730 family"
                     /protein_id="HRE03849.1"
                     /translation="MTELTPEVQAAVEQLLAVLAPQRDKRLATELISAAAGMIADRPA
                     TLDLKIATSAVMEMREAYEMFAPYRDRRKVTIFGSARTKLDDPLYQQARDVADRLARH
                     DWMVITGAGPGIMQAAMEGAGREQSIGVSI"
     assembly_gap    1272..1362
                     /estimated_length=91
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1363..>1654
                     /locus_tag="PLV68_21315"
     CDS             <1363..>1654
                     /locus_tag="PLV68_21315"
                     /inference="COORDINATES: protein motif:HMM:NF015597.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HRE03850.1"
                     /translation="MLVKESAAFVCLPGGFGTLDEMFELLTLTQTGKGVPVPIVFLDT
                     PGDPYWEQVAEFVDTQLVARSLVSPADTKLYLVTDSCDAAVDEISHFYSNFDS"
BASE COUNT          257 a          537 c          516 g          253 t
ORIGIN      
        1 gaccgctatg cggccgtgat cgaggcctac atcgccggtc tggagcagta cgccgcggac
       61 cccgacgccg atctcagcag ggtcgccagc gtggccagct tcttcatcag ccgggtcgac
      121 accgagatcg acaagcgtct ggaggcgctg ggaacgcccg aagcgctggc gttgcggggc
      181 aagggcgccg tggcgcaagg caagctggcc tacgcgatgt tcgacgccgc gttcagcacc
      241 gagcggtggc gggctcttgc cagccgtggt gcccgcgtcc agcgtccctt gtgggcctcg
      301 accagcacca agaaccccgc ctatcccgac acgctctacg tcgacgagtt gatcggcccg
      361 cacacggtca acacgttgcc cgagccgacg ctggaagcct ttgccgacca cggcaccttg
      421 gcccgccgcg tcgacgccga tctcgatgag gccacccggg cgtggcagca gctggccgcg
      481 gtcggcatcg acctcgacga cgtcgccgcg gtgctggaac gcgagggcgt cgcctcgttc
      541 cagcagagct tcgacgagtt gctggccgcc ttggccacca aggccgacga gttgcgcaac
      601 ggctgagtat ctcgccagca cgacggtgag gtgagtggca ccatccgccg gtgccactca
      661 ccccgtcgtg gaggcaggct gccggggacg gcgccagcct gtcatcccca tcgacacggg
      721 cagttgccga tggaggtttc ccgcacgatt gaacgagcga ttggggccgg cacctgctgt
      781 gctgcatgtg ccgtacgacc ttcaagaggg gttggcccgg tcgctatgac gcgacttggg
      841 aacttttccg agaaatttcc tacagtgcct gcgtgatgac tgaactcacg cccgaggtcc
      901 aggccgccgt cgagcagttg ctggccgtcc tggcgcccca gcgcgacaag cgcctcgcca
      961 ccgaactgat cagcgcggct gctggcatga tcgccgaccg tccggccacg ctcgatctca
     1021 agatcgccac gtcggcggtg atggagatgc gcgaggcgta cgagatgttc gccccgtacc
     1081 gggaccggcg caaggtgacc atcttcggtt cggcccgcac caagctcgac gacccgttgt
     1141 accagcaggc acgcgatgtc gccgaccggc tcgctcgcca cgactggatg gtcatcaccg
     1201 gtgccgggcc gggcatcatg caggcggcga tggagggtgc cgggcgcgag cagagcatcg
     1261 gcgtgtcgat cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngatgctcg tcaaggagag
     1381 tgccgccttc gtgtgcttgc ccggtggctt cggcacgctc gacgagatgt tcgaactgct
     1441 caccctgacc cagaccggca agggcgtgcc ggtgccgatc gtgttcctcg acacccctgg
     1501 cgacccgtac tgggaacagg tcgccgagtt cgtcgacacc caactcgtcg cccgctcgct
     1561 ggtgtcgccg gccgacacca agctgtacct ggtgaccgac tcgtgcgacg ccgcggtcga
     1621 cgagatcagc cacttctaca gcaacttcga cagc
//
LOCUS       DAORNF010000781         1653 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_52476_length_1653_cov_1.529412, whole genome shotgun sequence.
ACCESSION   DAORNF010000781 DAORNF010000000
VERSION     DAORNF010000781.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52476_length_1653_cov_1.529412"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>640
                     /locus_tag="PLV68_21320"
     CDS             <1..>640
                     /locus_tag="PLV68_21320"
                     /inference="COORDINATES: protein motif:HMM:NF015047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HRE03851.1"
                     /translation="AIVVQGGQGGGHTGNVATSILLPQVVDAVGGRIPVVAAGGFFDG
                     RGLVAALAYGADGVAMGTRFLLSSDSAVPDAVKQRYLGAGVADTVVSRDVDGVPHRVL
                     ATELVEQLGRRRWLALPRALRNAQRFRALTKTPYRQMLREGLAMRRSMDLTVAQMIMA
                     ANTPMLLKAALVDGKVESGVMASGQVVGVIDDLPSCQEIVAGIIADAREVLAR"
     assembly_gap    641..703
                     /estimated_length=63
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<704..1233)
                     /locus_tag="PLV68_21325"
     CDS             complement(<704..1233)
                     /locus_tag="PLV68_21325"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HRE03852.1"
                     /translation="MRGNIGGAVVHVPQVSNAAYMLGLVLQSKRVGLRDVGGALKHIE
                     PICAALCAERPDRARAVLPRLRALHQAALECIDSDELRFTELTREFHEAIVASCGNQT
                     MIELVGALETLWSAHEHEWATRITSDGAFPHHPVRRNSVTIHEQLLRLIEQGDAEAVA
                     LAARRHLDDGVFYASG"
     assembly_gap    1244..1253
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1254..1481)
                     /locus_tag="PLV68_21330"
     CDS             complement(<1254..1481)
                     /locus_tag="PLV68_21330"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRE03853.1"
                     /translation="MSSTSPAKMHLRQPRLAEMVADVLRQRILSGELGDGSIIGKQEE
                     LIEEFNVSKPSIREALRILETEGLISVQRGKL"
BASE COUNT          238 a          498 c          568 g          276 t
ORIGIN      
        1 acgccatcgt ggtgcaaggc ggccagggtg gcgggcacac cggcaacgtg gcgacgtcga
       61 tcctgctccc gcaggtggtc gatgccgtcg gtggtcgcat cccggtcgtc gctgcgggtg
      121 ggttcttcga cggtcgtggc ctggtggccg cgttggccta cggggccgac ggggtcgcga
      181 tgggcactcg ctttctgctg tcgagcgact cggccgtgcc cgacgcggtc aagcagcggt
      241 acctcggcgc gggcgtggcc gacaccgtgg tctcgcgcga cgtcgacggc gtaccgcatc
      301 gcgtgctggc gaccgaactg gtcgaacagt tgggtcggcg gcggtggctc gctctgccgc
      361 gggcgttgcg caacgcccag cgcttccggg cactgaccaa gaccccgtat cgccagatgc
      421 tgcgcgaggg gctggcgatg aggcgctcga tggatctgac cgtggcccag atgatcatgg
      481 cggccaacac gccgatgttg ttgaaggccg cgctggtgga cggcaaggtc gagagcgggg
      541 tcatggcgag cggccaggtg gtcggtgtga tcgacgacct gccgagctgt caggagatcg
      601 tcgccggcat catcgccgat gcgcgcgagg tgctggcccg nnnnnnnnnn nnnnnnnnnn
      661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntcgcctg acgcgtagaa
      721 cacgccgtcg tcgagatggc gacgagccgc aagcgcgacc gcttcggcgt cgccctgctc
      781 gatcagccgg aggagctgtt cgtggatcgt caccgagttg cgccgcacgg ggtgatgggg
      841 gaacgcaccg tcgctggtga tgcgcgtggc ccactcgtgc tcgtgtgccg accacaacgt
      901 ctccagcgcg ccgacgagtt cgatcatcgt ctggttgccg cacgacgcga cgatcgcctc
      961 gtggaactcg cgggtgagtt ccgtgaagcg caactcgtcg gagtcgatgc actccagggc
     1021 cgcttggtgt agcgcccgca gccgggggag cactgctctg gcccggtcgg gtcgctcggc
     1081 gcacagggca gcgcagatcg gctcgatgtg cttgagcgcg ccaccgacgt cgcgcagccc
     1141 gacccgcttc gactgcaaca ccaggccgag catgtaggcc gcgttcgaca cctgtggcac
     1201 gtgcaccacg gcaccgccga tgttgccgcg caagaccgtg atcnnnnnnn nnncagcttg
     1261 ccgcgctgca cgctgatgag accttcggtc tcgaggatgc gcagcgcttc ccggatcgac
     1321 ggcttggaga cgttgaactc ctcgatcagc tcctcttgct tgccgatgat gctcccgtcg
     1381 cccaactcgc cggacaggat ccgctggcgg aggacgtcag cgaccatctc ggcgagccgt
     1441 ggctggcgaa ggtgcatctt ggccggcgat gtggagctca cggatcctcg gttctgcagg
     1501 gctgggaaag ggtcagacct cgtcgttcag tgtccgttgc cacctggggc gggtagtctg
     1561 ctaaagtctg gattatatag atgattgctc tgagtccctc agggaccgag cgagacctga
     1621 gggagaccac agatggcgtc aaggatcgac ccg
//
LOCUS       DAORNF010000782         1653 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_52481_length_1653_cov_1.274718, whole genome shotgun sequence.
ACCESSION   DAORNF010000782 DAORNF010000000
VERSION     DAORNF010000782.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52481_length_1653_cov_1.274718"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(85..>378)
                     /locus_tag="PLV68_21335"
     CDS             complement(85..>378)
                     /locus_tag="PLV68_21335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03854.1"
                     /translation="QRLHGAGDAVPGPREIARGKDAAHAALVLVIDPEVGAERGVEGL
                     DPDTVGGRSESTIVTVRPARTRCMAALRPGKLPPMKSSRGGSAQLIGILRGAR"
     assembly_gap    379..455
                     /estimated_length=77
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            495..579
                     /locus_tag="PLV68_21340"
     tRNA            495..579
                     /locus_tag="PLV68_21340"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:529..531,aa:Leu,seq:tag)
     gene            634..>1256
                     /locus_tag="PLV68_21345"
     CDS             634..>1256
                     /locus_tag="PLV68_21345"
                     /inference="COORDINATES: protein motif:HMM:NF017508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HRE03855.1"
                     /translation="MQVTETLNEGLKRKLSVVIPKNDLSTRLDARLGELKDRANIKGF
                     RPGKVPTTYLKKVYGKSAMAEVMQDAINATVSDALTERSERAAAQPKVDLPEDQSVLN
                     QVLDGESDLSFDVSYEVLPAVELMDFHGLKLDRPVVEIADADVDKEMQRVFRQNRGYE
                     PKGEDGVVETGDNLGLSFVGKIDGKPFAGGSSDHAHLIVGSGDFIPG"
     assembly_gap    1257..1379
                     /estimated_length=123
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1380..>1653
                     /locus_tag="PLV68_21350"
     CDS             <1380..>1653
                     /locus_tag="PLV68_21350"
                     /inference="COORDINATES: protein motif:HMM:NF017509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03856.1"
                     /translation="ILHIDAPNAGELNDDFAKRLGLESLESLRSTVKDQMTAALASMS
                     RQAIKRQILDALDEGHKFDVPQQLVEAEFNTIWERVKHEVEHHGKSF"
BASE COUNT          287 a          482 c          432 g          252 t
ORIGIN      
        1 cactatcgaa tgttgtggtt gatggggcga tcggtgtcgt tgcctagaaa ttgtgcaacg
       61 tgcccagttc ttgctcaagt ctggtcatcg ggcgccgcgc aaaatgccga tcaattgtgc
      121 ggaacctccg cggcttgact tcatcggcgg cagcttcccc ggtctcagcg cggccatgca
      181 tctggttcgc gccggccgca cggtcacgat cgtcgactcc gatctgcccc caaccgtatc
      241 gggatcgaga ccttcgacgc cccgctcggc ccctacctca ggatcaatga cgagtacgag
      301 agcagcgtgc gcggcatctt tgccgcgggc gatctcgcgg ggccccggta cagcatcacc
      361 ggcgccatgc aggcgctgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnatttc atatatctga cccgccagcc
      481 cggccgcagg gcgtgcgggt gtggcgaaac tggtagacgc aacggattta ggttccgtcg
      541 ccgcaaggcg tgtaggttca agtcctatca cccgcaccag ctcgccggtt tcccgtccgc
      601 ctcggcacca cgaaacacaa acggaatgtc tctatgcagg tcaccgaaac cctcaatgaa
      661 ggcctgaagc gcaagctgag cgtggtcatc cccaagaacg accttagcac ccggctcgac
      721 gctcgcctcg gtgagctcaa ggaccgcgcc aacatcaagg gcttccggcc cggcaaggtg
      781 ccgaccacct acctcaagaa ggtctatggc aaatcggcca tggccgaagt gatgcaggac
      841 gccatcaacg ccaccgtctc ggatgcgctg accgagcgct ccgagcgcgc tgcggcgcag
      901 cccaaggtcg acctgccgga agaccagagc gtcctcaacc aggtgctcga tggcgaatcc
      961 gatctgagct tcgacgtctc ctacgaagtc ctgccggccg tcgagctgat ggacttccat
     1021 ggcctcaagc tcgaccgtcc ggtggttgag atcgccgacg ccgatgtcga caaggaaatg
     1081 cagcgcgtgt tccgccagaa ccgcggctac gagcccaagg gcgaagacgg cgtcgtcgag
     1141 accggcgaca atctcggcct gagctttgtg ggtaagatcg acggcaagcc cttcgcgggc
     1201 ggctcgtcgg accacgccca cctgatcgtc ggctcgggcg acttcatccc cggcttnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna
     1381 tcctccacat cgatgctccc aatgccggcg agctcaacga cgatttcgcc aagcgcctgg
     1441 gccttgagag cctcgaatcc ctgcgctcga cggtgaagga ccagatgacc gccgcgctcg
     1501 cgtcgatgag ccgccaggcc atcaagcggc agatccttga tgcactcgat gaaggccaca
     1561 aattcgacgt gccgcagcag ctcgtcgaag ctgagttcaa caccatctgg gagcgcgtga
     1621 agcacgaggt cgagcatcac ggcaagagct tcg
//
LOCUS       DAORNF010000783         1645 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_52779_length_1645_cov_0.947170, whole genome shotgun sequence.
ACCESSION   DAORNF010000783 DAORNF010000000
VERSION     DAORNF010000783.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52779_length_1645_cov_0.947170"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>220)
                     /locus_tag="PLV68_21355"
     CDS             complement(<1..>220)
                     /locus_tag="PLV68_21355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03857.1"
                     /translation="MLDVHAARCTDAMKLAAAEALAALVTEPTTTRILPGAFDPGVAD
                     AVADVVSTGATLASAGLEIFGPVILESTA"
     assembly_gap    221..455
                     /estimated_length=235
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<456..>678)
                     /locus_tag="PLV68_21360"
     CDS             complement(<456..>678)
                     /locus_tag="PLV68_21360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HRE03858.1"
                     /translation="ATVVGKPFESLRVVIVGAGAAGLASAKLLHGAGVADVIGVDREG
                     ILHPGVLTPESSVAKWWFAEHGNREGRTGC"
     assembly_gap    679..866
                     /estimated_length=188
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<867..1466)
                     /locus_tag="PLV68_21365"
     CDS             complement(<867..1466)
                     /locus_tag="PLV68_21365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03859.1"
                     /translation="MLSITNSVITTAPQPTAARVPARPTTPSAGFALRIELAPIVDVA
                     GLMRAVMATGADVRAMSTADDGSRVMTVNCTGIGQQDAVRAAIGTTPGARARSIEDAT
                     FAMHRGGKLRVAPKAPIAGPDELAMAYTPGVGRVSAAIAADPSLSWTFTGRSNTVAVL
                     TDGSAVLGLGNIGPEAAMPVMEGKAALFHQFAGIDAYPIC"
BASE COUNT          188 a          447 c          418 g          169 t
ORIGIN      
        1 ggcggtcgac tcgaggatca ccggcccgaa gatctcgagc ccggccgagg cgagcgtcgc
       61 ccccgtcgag acgacgtcgg cgaccgcgtc ggccacgccc gggtcgaagg cgccgggcag
      121 gatgcgcgtg gtcgtcggtt cggtcaccag cgcggccagg gcctcggccg cggccagctt
      181 catcgcgtcg gtgcagcgcg cggcgtgcac gtcgagcatg nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngcaac cggtacggcc ctcacggttg
      481 ccgtgctcgg caaaccacca cttggcaacc gagctctccg gcgtcaacac accagggtgg
      541 aggatgccct cgcggtcgac accgatcacg tcggccacac ccgcaccgtg cagcagcttg
      601 gccgaagcca gcccggccgc acccgcgccc acgatcacca cccgcagcga ctcgaacggt
      661 ttaccgacca cggtcgcgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      841 nnnnnnnnnn nnnnnnnnnn nnnnnngcag atcgggtagg catcgatgcc cgcgaactgg
      901 tggaacagcg cggccttgcc ctccatcacc ggcatcgccg cctctggtcc gatgttgccc
      961 aggcccagca cggcgctgcc gtcggtgagc accgcaacgg tgttgctacg tcccgtgaag
     1021 gtccagctca aggacggatc agcagcgatg gccgcggaca cccgccccac accgggcgtg
     1081 taggccatcg ccaactcgtc cggcccggcg atcggcgcct tcggagcgac acgcagcttg
     1141 ccgccgcggt gcatggcaaa ggtcgcgtcc tcgatcgagc gcgcccgcgc tcccggcgtc
     1201 gtgccgatcg ccgcgcgcac ggcatcctgc tggccgatgc cggtgcagtt gaccgtcatc
     1261 acccgcgacc cgtcgtcggc ggtgctcatg gcgcgcacgt cggcaccggt cgccatcaca
     1321 gcccgcatca gccctgccac gtccacgatc ggcgccagtt cgatccgcag tgcgaacccg
     1381 gccgacggcg tggtgggacg cgctggcaca cgcgcggccg tcggctgcgg cgcggtggtg
     1441 atgactgagt tggtgatcga caacattggc aatcctcttc gggtacatcg agtggattgc
     1501 cggacaaccg tcagcgtcac cgccgccgga gcccggcggg gtggaacagt gggatcgtga
     1561 cgctcagcgc gtgcggcgaa cccgtgtgcc acgcccactg cgaacaagtg cgggaatggc
     1621 tgcgggtacg cgtgctgctg cggcg
//
LOCUS       DAORNF010000784         1644 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_52795_length_1644_cov_1.741976, whole genome shotgun sequence.
ACCESSION   DAORNF010000784 DAORNF010000000
VERSION     DAORNF010000784.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1644
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52795_length_1644_cov_1.741976"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>848)
                     /locus_tag="PLV68_21370"
     CDS             complement(<1..>848)
                     /locus_tag="PLV68_21370"
                     /inference="COORDINATES: protein motif:HMM:NF020390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1800 family protein"
                     /protein_id="HRE03860.1"
                     /translation="YATLAQAMAIEPAMLDYLDNRANSRKSPNQNFARELLELFLLGV
                     GNYAESDVEAAAAAWTGHTIDDDGNYWFRNDRHDNGNKTFFGITKNWNGPDIITEVLT
                     GAKRQIAARHVVGKLWDFFAAPNTRPAALEAIATSFAATWDIKAALRAILLRDEFYSQ
                     PVRSGLVRTPVDWVVSVLVHTGYRAGAVNPQWYMEGMGQVPFNPPNVSGWRSNSAWVN
                     ASAMGARADFARNVTWRLRNKDIAENQSRPAHWGPDFALLTKRTIPQAVADMAGFFGL
                     GTLSAP"
     assembly_gap    849..905
                     /estimated_length=57
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<906..1194)
                     /locus_tag="PLV68_21375"
     CDS             complement(<906..1194)
                     /locus_tag="PLV68_21375"
                     /inference="COORDINATES: protein motif:HMM:NF020390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 family protein"
                     /protein_id="HRE03861.1"
                     /translation="MPDQATTEHLLRRTEYVARPARVAELLSLGLEQAVDNVLTPVGP
                     VALPAYLDHNIEGEGWNQYVAAMKWWFDRMAFDSPRPVHEKMTFFWHGHFTS"
     gene            complement(1407..1619)
                     /gene="rpmE"
                     /locus_tag="PLV68_21380"
     CDS             complement(1407..1619)
                     /gene="rpmE"
                     /locus_tag="PLV68_21380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014697411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HRE03862.1"
                     /translation="MQTDIHPAYAELTVKCTCGNTFVTRSTKGGSMNVELCNECHPFF
                     TGKQKLVDSGGRVERFNRRYGARKSG"
BASE COUNT          255 a          491 c          554 g          287 t
ORIGIN      
        1 cggggccgac agcgtgccga ggccgaagaa gccggccatg tcggcgacgg cctgcgggat
       61 ggtgcgcttg gtcagcaacg cgaagtcggg accccagtgc gcggggcggc tctggttctc
      121 ggcgatgtcc ttgttgcgca gccgccatgt cacgttgcgg gcgaaatcgg cccgagcgcc
      181 catcgcgctg gcgttgaccc acgccgaatt cgaccgccag cccgacacgt tcggaggatt
      241 gaacggcacc tgccccatgc cttccatgta ccactgcggg ttgaccgcgc ctgcgcggta
      301 gccggtgtgc accaggaccg acaccaccca gtcgaccggg gtacgcacca gtccgctgcg
      361 caccggttgg gagtagaact cgtcgcgcag caagatcgcc cgcagcgcgg ccttgatgtc
      421 ccacgtggcc gcgaacgagg tggcgatcgc ctccaacgct gcggggcggg tgttcggcgc
      481 agcgaagaag tcccacagct tgccgaccac gtgacgggcg gcgatctggc gcttggcacc
      541 ggtcagcact tcggtgatga tgtcgggacc gttccaattc ttggtgatgc cgaagaaggt
      601 cttgttgccg ttgtcgtggc gatcgttgcg gaaccagtag ttgccgtcgt cgtcgatggt
      661 gtgcccggtc caggccgcgg cggctgcttc gacgtcggat tcggcgtagt tgccaacgcc
      721 gagcaggaac agctcgagca gctcgcgggc gaagttctgg ttgggcgact tgcgcgagtt
      781 ggcccggttg tcgaggtagt cgagcatcgc cggttcgatc gccatcgcct gagccagcgt
      841 ggcgtagtnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      901 nnnnncgctg gtgaaatggc cgtgccagaa gaaggtcatc ttctcgtgca ccggccgcgg
      961 cgaatcgaag gccatccggt cgaaccacca cttcatggcg gccacgtact ggttccagcc
     1021 ctcaccctcg atgttgtggt cgaggtaggc gggcagcgcc accggcccga cgggtgtcaa
     1081 cacgttgtcc accgcttgct ccaaacccag cgacaacagc tccgcgacgc gggccggacg
     1141 ggcgacgtac tcggtgcgac gcagcagatg ttcggtggtt gcctggtcgg gcatggcggt
     1201 ggcacacgtc ctgttgatcg ggtcggctca gtatcggcga ccacaccgcg accctgaagt
     1261 cagggattcg tcaggattcg tgctgtcccc cgcagatcga gcacagacgc agaactgcca
     1321 ccccgagtga ggtggcagtt ccagaacgtt cggttgtcgt gcgagcgccg gaggcgccgc
     1381 tggcgtgcca aatctggcgt tgcggttcag ccggacttgc gggcgccgta gcggcggttg
     1441 aagcgctcga cgcggccacc cgagtcgacc agcttctgct tgccggtgaa gaacgggtgg
     1501 cattcgttgc agagctcgac gttcatgctg ccgcccttgg tgctgcgggt aacgaacgtg
     1561 ttaccgcagg tgcacttgac ggtgagctcg gcgtaagcgg ggtgaatgtc ggtctgcatg
     1621 acgtgctcca acgtgggcga atcg
//
LOCUS       DAORNF010000785         1630 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_53268_length_1630_cov_1.398730, whole genome shotgun sequence.
ACCESSION   DAORNF010000785 DAORNF010000000
VERSION     DAORNF010000785.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53268_length_1630_cov_1.398730"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>218
                     /locus_tag="PLV68_21385"
     CDS             <1..>218
                     /locus_tag="PLV68_21385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRE03863.1"
                     /translation="KSYGTHEVLHGIDLTVNAGEVAVILGASGSGKSTLLRCINHLEK
                     VNRGVISIDGDTVGYRRVGNTLHELRER"
     assembly_gap    219..380
                     /estimated_length=162
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <381..720
                     /locus_tag="PLV68_21390"
     CDS             <381..720
                     /locus_tag="PLV68_21390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002183294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRE03864.1"
                     /translation="RADSYPRQLSGGQQQRVAIARALAMEPKVLLFDEPTSALDPEMV
                     NEVLDVMKSLAQAGMTMIVVTHETGFAREVADTVVFMDDGVIVEAGPAAAVLDHPQHE
                     RTAAFLAKVL"
     gene            794..>1630
                     /locus_tag="PLV68_21395"
     CDS             794..>1630
                     /locus_tag="PLV68_21395"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HRE03865.1"
                     /translation="MNRTVNHADRRVGALSRLHGRNCLAALALTSMLVVAACGSDGDS
                     AGNVSTTVGSTSTSATPTSTEPASTDSTSPAAAASTTTNSAAPVAVVDGSGFDTTPEQ
                     ATRVRVDKVDEIAATLPQHWKDTGKLVVGVGAFDAPPILLYATDETTIIGNELDVAYL
                     VADTLGLELVQKDSSWENLFLSVESGQFDVGFANITVTEERKEKYDFASYRVDSVAFE
                     AIAGSDLVIAEPADIAGLKVAVGSGTNQEQILLAWDAANQAAGLKAADIQYYQNEADF
                     LLA"
BASE COUNT          268 a          509 c          466 g          225 t
ORIGIN      
        1 caagagctac ggcacccatg aggtgctcca cggcatcgac ctcacggtga acgcgggtga
       61 ggtcgcggtc atcctgggcg cgtccggttc gggcaagagc accctgttgc gctgcatcaa
      121 ccatctcgaa aaggtcaacc gtggcgtgat cagcatcgac ggagacaccg tcggctatcg
      181 ccgggtgggc aacacgctgc atgagttgcg cgagcgcgnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn ccgcgccgac tcgtacccgc ggcaattgtc cggcggccag
      421 cagcagcggg tcgccatcgc ccgcgccctg gcgatggagc cgaaggtctt gctcttcgac
      481 gagccgacca gtgcgctcga cccggagatg gtcaacgaag tcctcgacgt gatgaagagc
      541 ctggcccagg cgggcatgac gatgatcgtc gtgacccacg agacgggttt cgcgcgtgag
      601 gtcgccgaca ccgtggtgtt catggacgac ggcgtgatcg tcgaggccgg cccggcagcc
      661 gccgtgctcg atcacccgca acacgagcgc acggcggcgt tcctggccaa ggtgctctga
      721 gcgcgggcaa cgcccgcgac ccgccaaacc acccccaaac ccaaccccca acccctgata
      781 ctcgagtcaa cgcgtgaacc gcactgtgaa ccatgccgat cgtcgagtcg gcgccctttc
      841 ccgcctgcac ggccggaact gcctggccgc gctcgccctc acctcgatgc tggtcgttgc
      901 ggcctgtggc tcggatggtg attcggccgg gaacgtgagc accaccgtcg ggtcgacatc
      961 gacctctgca acccccacct cgaccgaacc ggcctcgacg gactcgacct cgcctgcggc
     1021 ggccgcgagc acgaccacca actccgctgc gcccgtagct gttgtcgacg gcagcgggtt
     1081 cgacacgacg cccgagcaag ccacgcgcgt cagggtcgac aaggtggacg agatcgccgc
     1141 cacgttgcca cagcactgga aggacacggg caagctcgtc gtcggtgtcg gtgctttcga
     1201 cgcgccgccg atcttgctct acgccaccga cgagacgacg atcatcggca acgaattgga
     1261 cgtcgcctac ctcgtcgccg acacgctcgg tctggaactg gtgcagaagg acagctcgtg
     1321 ggagaacctg ttcctctccg tcgagagcgg ccagttcgac gtcggtttcg ccaacatcac
     1381 ggtgaccgag gaacgcaagg agaagtacga ctttgcctcg tatcgcgtcg actcggtggc
     1441 cttcgaagcc atcgccggca gcgacctcgt gatcgccgag ccggccgaca tcgccgggtt
     1501 gaaggtcgcc gtcgggtctg gcaccaacca ggagcagatc ctgctggcct gggatgccgc
     1561 caatcaggcg gcggggctca aagcagccga catccagtac taccagaacg aggccgactt
     1621 cctgctcgcg
//
LOCUS       DAORNF010000786         1627 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_53378_length_1627_cov_2.148855, whole genome shotgun sequence.
ACCESSION   DAORNF010000786 DAORNF010000000
VERSION     DAORNF010000786.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53378_length_1627_cov_2.148855"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            270..>1627
                     /locus_tag="PLV68_21400"
     CDS             270..>1627
                     /locus_tag="PLV68_21400"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRE03866.1"
                     /translation="MSLNTTELRARSRAQLAATAITIERGGRTILDNVSLTVTPGTRL
                     GIVGENGRGKSSLLHVLSGSLAATRGTIRRVGSLGIAEQEINVDPTRTVGQLIDIELA
                     DARAALRALDDATVALAEGRSDAETRYADALDAATALDAWDADRRVDLALDALGAITD
                     RQRRLAEMSVGERYRVRLACLLGAEHDFLLLDEPTNHLDRQALDFLTAALRSSEGGVV
                     VVSHDRVLLADVATKVLDLDPSRDGRPRLYGNGYDGYVAGRTSELARWEQEYEQHQSE
                     QARLANDLSTAQNRLQSGWRPPKGTGKHQRATRAPSLVRAVNRRLDDLATHSVAKPVA
                     PLRFQMPDLPTLPGTTLVHVEAVTVADRLETPVSMTLESNDHRLVTGPNGAGKSTLLA
                     VLAGQLQPDTGRVVHAPHARIGLVAQESPSHDRRSVHTVYEHRLQQLRSHDPTTPTLS
                     LRA"
BASE COUNT          266 a          643 c          493 g          225 t
ORIGIN      
        1 cggcgccatt tgcccgctcg ctgcgatcac gctcggctcg gctgacacga catccttgcc
       61 gtgacgagag agccagccgg cggtggtcgg agacctggcc ggtatcgtgg gtgtcgtgca
      121 attcctgcac ttttggtgat ctcgacgggc gttcccgcgg ccttcggccg tgaggcgaac
      181 ccgcggcccg cgacggcggg tcggtcgtac ctgatcaccc tgacgcctcg ccctctggcg
      241 gcgggcgtct gccacagaga agtgcagaga tgtccctcaa caccaccgaa ctccgggcgc
      301 gctcgcgcgc ccaactggcg gccaccgcga tcaccatcga gcgcggcggc cgaaccatcc
      361 tcgacaacgt ttccctcacc gtcacaccgg gcacgcggct cgggatcgtc ggcgagaacg
      421 gccgcggcaa gtccagcctg ctccacgtcc tgtccggctc actggccgcc acacgcggca
      481 cgatccgccg ggtcggaagc ctcggcatcg ccgaacaaga gatcaacgtt gacccgaccc
      541 gcaccgtcgg gcaactgatc gacatcgaac tcgccgacgc gcgtgccgcg ctgcgcgcgt
      601 tggacgacgc cacggtcgcc cttgccgagg gccgatccga cgccgagacg cgctatgccg
      661 atgcgctcga cgcggcgacc gcgctcgacg cgtgggatgc cgaccgtcga gtcgacctcg
      721 ccctcgacgc gctcggcgcg atcaccgacc gccagcgacg tctggcggag atgtcggtcg
      781 gtgagcgata ccgcgtccgc ctggcgtgcc tgctcggcgc cgaacacgat ttcttgctgc
      841 tcgacgaacc caccaaccac ctcgaccgcc aggccctcga cttcctcacc gccgctctac
      901 gcagcagcga aggtggcgtc gtggtggtca gtcacgatcg ggtcctgctc gccgacgtcg
      961 ccaccaaggt gctcgacctc gaccccagcc gcgacggccg gccccggctg tacggcaacg
     1021 ggtacgacgg ctacgtcgcg ggacggacct cggagctcgc ccgctgggag caggagtacg
     1081 aacagcacca atccgagcag gcgcggttgg ccaacgacct gtcgaccgcc cagaaccggc
     1141 tgcagtccgg ctggcgccca cccaaaggca ccggcaagca ccagcgcgcc acccgcgccc
     1201 cgtcactcgt tcgcgccgtc aaccgccgcc tcgatgacct cgccacccac tcggtcgcca
     1261 aaccggtggc accgctgcgg ttccagatgc ccgacctgcc gacacttcct ggcaccaccc
     1321 tcgtgcacgt cgaagccgtc accgtcgccg atcgtctcga gacgccggtg tcgatgacgc
     1381 tcgagtcgaa cgatcaccga ctcgtcaccg gccccaacgg tgccggcaaa tcgaccctgc
     1441 tcgcggtgct cgccgggcaa ctccaacccg ataccggccg cgtcgtgcac gcaccgcacg
     1501 cgcgcatcgg tctggtcgcc caagagtcac cgtcgcacga ccgacgcagc gtccacaccg
     1561 tctacgaaca ccgactccag caactccgat cccacgaccc gaccacgccg accctgtcgc
     1621 tgcgcgc
//
LOCUS       DAORNF010000787         1617 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_53767_length_1617_cov_1.902049, whole genome shotgun sequence.
ACCESSION   DAORNF010000787 DAORNF010000000
VERSION     DAORNF010000787.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53767_length_1617_cov_1.902049"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>374
                     /locus_tag="PLV68_21405"
     CDS             <1..>374
                     /locus_tag="PLV68_21405"
                     /inference="COORDINATES: protein motif:HMM:NF020919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1996 domain-containing protein"
                     /protein_id="HRE03867.1"
                     /translation="DVNGAPQAPVTGAQGKTPQFLVECPYSHAAPDDPIVYPGQPGRS
                     HMHLFFGNEDADAFSTADSLLTGSSLCDQRLDTASYWAPALYDGDRMVTPAKSVAYYR
                     AGAGVDPASIQPYPFGLQVIAG"
     assembly_gap    375..387
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <388..786
                     /locus_tag="PLV68_21410"
     CDS             <388..786
                     /locus_tag="PLV68_21410"
                     /inference="COORDINATES: protein motif:HMM:NF020919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1996 domain-containing protein"
                     /protein_id="HRE03868.1"
                     /translation="PQSNAVVAWTCGTGITRDVEPPTCPVSRPLRFLVTFPDCWDGEH
                     LVADDHMSHMAYSSGGRCPVTHPVPVPQLQFSIAYGFSGDPSGLRLASGSVLTGHADF
                     FNAWHEEELADEIANCLHRGVVCGITSGRE"
     gene            928..>1617
                     /locus_tag="PLV68_21415"
     CDS             928..>1617
                     /locus_tag="PLV68_21415"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRE03869.1"
                     /translation="MRKFNKIAALGIAGALVLGACGSDEKKSDGPATGTVDCKPIKAG
                     VLSVVTSLPGPNFWGTPAGETDPNQIKSGIEYDIATKIAETCGLKMEYRNESFDAIVA
                     GQIPENSYDIALSQVTITDDRAKVVDFSDGYFKSDQGLLVRKGTTVGSWADVKAMNLG
                     VQASTTAEYYLLEVPEWKTDGDVKSFVDLTSAYEALSSGQIDGVIIDTPINLGQAAQS
                     NGELEVVAQFVT"
BASE COUNT          277 a          544 c          524 g          259 t
ORIGIN      
        1 ccgacgtcaa cggtgcgccc caggcccccg tcaccggggc gcagggcaag acgccgcagt
       61 tcctggtcga atgcccgtac agccacgccg cgccggacga cccgatcgtg tacccgggcc
      121 agccgggccg ctcgcacatg cacctcttct tcggcaacga agatgccgac gcgttctcca
      181 ccgccgactc gttgctcacc ggttcgtcgt tgtgcgacca gcgactcgac accgcctcgt
      241 actgggcgcc cgcgctctac gacggcgacc gcatggtgac gccggccaag agcgtcgcct
      301 actaccgcgc cggcgccggc gtcgatccgg cgtcgatcca gccctacccg ttcggattgc
      361 aggtcattgc cggcnnnnnn nnnnnnnccg cagtcgaacg cggtggtcgc ctggacctgc
      421 ggtaccggca tcacccgcga cgtcgaaccg cccacgtgcc cggtgtcgcg cccgttgcgc
      481 ttcctggtca cgtttcccga ctgctgggat ggtgagcacc tcgtcgccga cgatcacatg
      541 agccacatgg cctacagcag cgggggccgt tgtccggtga cccatccagt cccggtgccg
      601 cagttgcagt tctcgatcgc ctacgggttc agcggtgacc ccagcggcct gcgtctggcc
      661 agcggttcgg tgctgaccgg tcacgccgac ttcttcaacg cgtggcacga ggaggagttg
      721 gccgacgaga tcgccaactg tctgcaccgc ggcgtggtgt gtggcatcac cagcggtcgg
      781 gagtgagtgg gagtgagtga ccggcgagaa gcgatcggtg ttacgtcccg ttcacgccgc
      841 ctcggcctgg tggatcggac gcgcagtagg gtacggcttc acttcggggg agaggcccgt
      901 gaggccgcat cgacagatgg aggaaaaatg aggaagttca acaagattgc agcgttgggc
      961 atcgccggag cgttggtgct cggtgcctgc ggcagcgacg agaagaagtc cgacggcccg
     1021 gccaccggca ccgtcgactg caagccgatc aaggccggcg tgttgtcggt ggtcaccagc
     1081 ctgcccgggc cgaacttctg gggtacgccg gcgggcgaga ccgaccccaa ccagatcaag
     1141 agcggcatcg agtacgacat cgccaccaag atcgccgaga cctgcgggct caagatggag
     1201 tatcgcaacg agagcttcga cgcaatcgtc gccgggcaga tccccgagaa ctcgtacgac
     1261 atcgccctgt cgcaggtgac gatcaccgac gaccgggcca aggtcgtcga cttctccgac
     1321 ggctacttca agtccgacca gggcctgctg gtgcgcaagg gcaccacggt tggctcctgg
     1381 gctgacgtca aggcgatgaa cctcggtgtg caggcttcga ccacggccga gtactacctg
     1441 ctcgaggtgc ccgagtggaa gaccgatggc gacgtcaagt cgttcgtcga cctcacctcg
     1501 gcctacgagg cgctcagttc gggccagatc gacggcgtca tcatcgacac cccgatcaac
     1561 ctcggccagg ccgcccagtc gaacggtgag ctcgaagtgg tcgcccagtt cgtcacc
//
LOCUS       DAORNF010000788         1612 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_53982_length_1612_cov_1.124599, whole genome shotgun sequence.
ACCESSION   DAORNF010000788 DAORNF010000000
VERSION     DAORNF010000788.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53982_length_1612_cov_1.124599"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            102..425
                     /locus_tag="PLV68_21420"
     CDS             102..425
                     /locus_tag="PLV68_21420"
                     /inference="COORDINATES: protein motif:HMM:NF021996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DM13 domain-containing protein"
                     /protein_id="HRE03870.1"
                     /translation="MTEFISNAHSTSGKVLVVALADGSVVMRFEDLESDNGPDLRVVL
                     STSTLNSGGDYGGRLILEHLKGNIGSQNYTIGTDVDLGVYRSVVIWCERFGVAFGSAA
                     IDVTA"
     assembly_gap    564..575
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <576..>887
                     /locus_tag="PLV68_21425"
     CDS             <576..>887
                     /locus_tag="PLV68_21425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03871.1"
                     /translation="KVGDRVIDRVPAWLKNFAIENFGTSDKAVLIATILVVITASAVV
                     VGIATVRRAAWIGVVAVAVCAVLGALAGGWGRNTETVGAVPAVAAAAVALAVLLIGRR
                     LA"
     assembly_gap    888..897
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            921..>1612
                     /locus_tag="PLV68_21430"
     CDS             921..>1612
                     /locus_tag="PLV68_21430"
                     /inference="COORDINATES: protein motif:HMM:NF012401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRE03872.1"
                     /translation="MKILSAPLDRRRFLVGSGLLAVTALGVAGFGRQLQARFNSALER
                     AGVRLPRPTEPLPPLPADPADELANAGLTPLIMPIERFYRIDTALTFPDVSLDTWRLK
                     ITGLVGRELSFSYDDLVARDLIERDITLSCVSNEVGGSLVGNARWVGCRLDDLLDSAG
                     IDKRANQVMGQSLDGFTAGFPLDTLDGRDAMIAIGMNGEPLPLQHGFPARVIVPGLYG
                     YVSATKWLARLDL"
BASE COUNT          226 a          524 c          566 g          274 t
ORIGIN      
        1 ttcatccctg aatggaggag ccagcgggtc tgggtctcgg gcgaaggcga cgcgatggtc
       61 accgaggcgc cgcagcgacc ggtcagccag tgatcgtgtc gatgaccgag ttcatcagca
      121 acgcccactc gacgtccggc aaggtgctgg tggtcgcgct ggccgacggg tcggtggtga
      181 tgcggttcga ggacctcgag tccgacaacg gacccgacct gcgtgtggtg ctgtcgacct
      241 cgacgctcaa cagcggtggc gactacggcg gccggttgat cctggaacac ctcaagggca
      301 acatcggctc gcagaactac acgatcggta ccgacgtcga cctcggtgtc tatcgctccg
      361 tcgtcatctg gtgcgagcgg ttcggcgtgg ccttcggttc ggccgcgatc gacgtgaccg
      421 cgtgaacgtc gtgatgccat gaacgatgtg acgctgtgaa cgagagcgac caccgtccac
      481 cgcggtggtg gggagcgctc accgggctgc tcgccgcagc agccgggctg gccgtgggcg
      541 agttcgtcgc cggactgtct gaannnnnnn nnnnncaagg tcggcgaccg cgtcatcgat
      601 cgcgtcccgg cgtggctgaa gaacttcgcc atcgagaact tcggtaccag cgacaaggcg
      661 gtgttgatcg cgacgatcct ggtggtgatc accgcgagcg ccgtcgtggt cggtatcgcc
      721 accgtgcgcc gggcggcctg gatcggcgtc gtcgccgtgg cggtctgcgc cgtgctcggc
      781 gcgctcgccg gtggttgggg ccgcaacacc gaaacggtcg gtgccgtgcc cgcggtggcc
      841 gctgcagccg tcgcgttggc ggtgctgttg atcggtcgac ggttggcnnn nnnnnnntcg
      901 taccggtcgg cggcggcacg atgaaaatcc tctccgcgcc gctcgaccgg cgccgcttcc
      961 tggtggggtc ggggttgctg gcggtgaccg cgctgggcgt ggctggattc ggccgtcaac
     1021 tgcaggcccg cttcaacagc gcgctggaac gggctggggt gcgcctgccc cgccccaccg
     1081 agcccctgcc accgctgccg gccgacccgg ccgacgaact cgccaacgcc ggcttgaccc
     1141 cgctgatcat gccgatcgaa cggttctacc gcatcgacac ggccctcacc tttcccgacg
     1201 tctcgctgga cacgtggcgg ttgaagatca ccggcttggt cgggcgcgag ctcagcttca
     1261 gctacgacga cctggtcgcc cgtgacctga tcgagcgcga catcaccctg tcgtgcgtgt
     1321 cgaacgaggt cggcggttcg ttggtgggca acgcccgctg ggtggggtgc cgcctcgacg
     1381 atctgctcga ctcggccggg atcgacaagc gggccaacca agtgatgggc cagtcgctcg
     1441 acggcttcac cgctgggttc ccgctcgaca cgctcgacgg gcgcgacgcg atgatcgcga
     1501 tcggcatgaa cggcgagccg ctgccgctgc agcacgggtt cccggcgcgg gtgatcgtgc
     1561 ccggcctgta cggctacgtg agcgctacga agtggctcgc gcggctcgac ct
//
LOCUS       DAORNF010000789         1608 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_54119_length_1608_cov_1.752737, whole genome shotgun sequence.
ACCESSION   DAORNF010000789 DAORNF010000000
VERSION     DAORNF010000789.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54119_length_1608_cov_1.752737"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="PLV68_21435"
     CDS             complement(<1..211)
                     /locus_tag="PLV68_21435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03873.1"
                     /translation="MSAYLERHFEAELCEHLAAHGWLYSADDSLYDRDLALVPEDVFG
                     WLGDTQAEVLGKVVKAGSAGEAKQRA"
     gene            complement(208..>925)
                     /locus_tag="PLV68_21440"
     CDS             complement(208..>925)
                     /locus_tag="PLV68_21440"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HRE03874.1"
                     /translation="DRLIAKQTALIDTLRERRTAAIHALTFSLPRVDFRPVKQVADVF
                     IGKMLQPEQRSAEEVYAPYLRAAHVQPLGRLIDVDDKRMWFGVEELAAHDVRVGDVIV
                     VEGGAGFGRSAVVREDLLGWGFQKSVIRIRAKPEHAVGAFIDYALQAALAIGTVEMAA
                     STATIPHFTAEKVAVFPIPSPAVEEQRKIVEQLDEQTAKIDLLISKAERFVELARERR
                     AALITAAVTGQIEVGERGAA"
     assembly_gap    926..948
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<949..>1325)
                     /locus_tag="PLV68_21445"
     CDS             complement(<949..>1325)
                     /locus_tag="PLV68_21445"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HRE03875.1"
                     /translation="ITATTEFMLASARPEQIERFRLHIGDTVITKDSETAQDIGIPAF
                     VEYEADDLVCGYHLAIVRPEPRVANARYLYWCLSSSVMLRQWELAASGVTRVGIRSGA
                     LARAHLPAPPLEEQRRIADFLDA"
     assembly_gap    1326..1360
                     /estimated_length=35
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          273 a          535 c          450 g          292 t
ORIGIN      
        1 gcgcccgctg cttggcctcg cccgcggatc cggccttgac caccttgccc aagacctcag
       61 cctgtgtgtc gcccagccac ccgaacacat cctcgggcac caacgccaga tcacggtcgt
      121 agagcgaatc gtcggccgag tacaaccacc cgtgggccgc caggtgttcg cacaactccg
      181 cctcgaagtg ccgttccaga tacgcgctca cgccgccccc cgctcgccga cctcgatctg
      241 ccccgtcacc gccgcggtga tcaacgccgc ccgccgctcc ctggccagtt ccacaaaccg
      301 ctccgccttg gagatgagca ggtcgatctt cgcagtctgc tcgtccaact gctcaacgat
      361 cttgcgctgt tcttcgaccg ccggcgacgg aatcggaaag accgctacct tctccgcggt
      421 gaagtgggga atcgtcgccg tcgaggccgc catctcgacc gttccaatcg ccagggccgc
      481 ctgaagggcg tagtcaatga acgccccgac agcgtgttcc ggctttgccc ggatgcggat
      541 gacggacttc tgaaaccccc agccaagtag atcctcacga acgacggccg accgtccgaa
      601 tcctgctccg ccctcgacga cgatgacgtc gcccacgcgc acgtcgtgtg ctgcgagttc
      661 ttctacgcca aaccacatcc gcttgtcgtc gacgtcgata agccgcccga gcggctgaac
      721 gtgggcagcg cgaaggtatg gggcgtacac ctcctctgcg gacctctgtt ccggttgaag
      781 catcttcccg atgaagacgt ctgcgacctg tttgaccggg cgaaagtcaa ctctcgggag
      841 ggagaacgtc aatgcgtgga ttgcagcggt gcgccgttcg cggagggtgt cgatcagggc
      901 ggtctgcttg gcgatcagcc gatcgnnnnn nnnnnnnnnn nnnnnnnncg catcgagaaa
      961 gtccgcaatc cgccgctgct cctccaacgg gggcgcaggc aagtgcgctc gagccagcgc
     1021 tccactgcgg atacccactc gtgtgacacc tgaggccgcg agttcccatt gacgcagcat
     1081 caccgaggat gacaagcacc agtacagata gcgcgcgttt gccacccgtg gctctggccg
     1141 cacgattgcg aggtggtagc cgcagaccaa gtcgtccgcc tcgtactcga caaacgccgg
     1201 gataccgatg tcttgcgctg tctccgagtc cttcgtgatg accgtgtcgc cgatatgaag
     1261 ccgaaaccgt tcgatctgct caggtcgagc gctcgccaac atgaattccg tcgtcgccgt
     1321 gatcgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tgtagacgtc ggtgtagttg
     1381 cagagccgca ccggcgtttc cccctctacc gagagcttgt ccaccgtgct cgtgctgacc
     1441 cttgctgcga cgccgagtcg agtgagttgc caggaccgcg ggagacgtcc aatccaaggt
     1501 gaatcggtgt tgcatggtgc ggtgacgttc tcgctcaccg ctccacctcg cgcagcagct
     1561 cgatgatctc tgctgcgagc ttgttcaggt cggcgtcgat ctcctcca
//
LOCUS       DAORNF010000790         1602 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_54353_length_1602_cov_2.341952, whole genome shotgun sequence.
ACCESSION   DAORNF010000790 DAORNF010000000
VERSION     DAORNF010000790.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1602
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54353_length_1602_cov_2.341952"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..875)
                     /gene="metK"
                     /locus_tag="PLV68_21450"
     CDS             complement(<1..875)
                     /gene="metK"
                     /locus_tag="PLV68_21450"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HRE03876.1"
                     /translation="MSTWTFTSESVTEGHPDKMADQVSDAVLDAILTDDPNGRVACET
                     LLTTGLCVVAGEITTNAYVDIPKIAREVIKDIGYDNANYGFDGNTCGVIVSIDEQSAD
                     IAQGVDKSEELRGGKAGEDTINSQGAGDQGMMFGYACDETPDLMPLPIWLAHRLAERL
                     SEVRKSGAVPYLRPDGKTQVTFEYQHGKPVALKAVLVSTQHAEGIDRDTVIRPDLIEQ
                     VIRPTIPEQFADQAYDVFVNPTGVFVVGGPHGDTGLTGRKIIVDTYGGMGRHGGGAFS
                     GKDPSKVDRSAAYAAR"
     gene            complement(964..>1602)
                     /locus_tag="PLV68_21455"
     CDS             complement(964..>1602)
                     /locus_tag="PLV68_21455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HRE03877.1"
                     /translation="VVTAGGTREPIDAVRVVANRSSGKQGYAIAAEAAARGAQVTLIS
                     TVDLPVPVGVRVERVETAAQMQAAVERLAPNAGVVVMAAAVADFRPVAAAPNKLKKHD
                     GPPQIVLEPTPDILAGLGAVKPQGQVLVGFAAETDDLVANATAKLTRKNLDLIVANDV
                     GAANVGFQHDTNEVTLLAPHAAPVVVSLADKRSIARAVLDRVGEIRAGRVQP"
BASE COUNT          219 a          525 c          572 g          286 t
ORIGIN      
        1 cgggccgcgt acgccgccga acggtcgacc ttgctggggt ccttgccact gaacgcgccg
       61 ccaccgtggc gacccatgcc gccgtaggtg tcgacgatga tcttgcgtcc cgtcaggccg
      121 gtgtcaccgt gcggaccacc gacgacgaac acgccggtcg ggttgacgaa cacgtcgtag
      181 gcctggtcgg cgaactgttc cgggatcgtc ggacggatga cctgttcgat caggtcggga
      241 cggatcaccg tgtcgcggtc gatgccttcg gcgtgttggg tgctgaccag cacagccttg
      301 agcgccaccg gcttgccgtg ctggtactcg aacgtgacct gggtcttgcc gtcggggcgc
      361 aggtacggca ccgcgcccga cttgcgcacc tcggacagcc gctcggccag acgatgggcg
      421 agccagatcg gcagcggcat cagatcgggc gtctcgtcgc aggcgtagcc gaacatcatg
      481 ccctggtcgc cggcgccctg ggagttgatc gtgtcctcgc ccgccttgcc gccgcgcagc
      541 tcttcgctct tgtcgacgcc ttgggcgatg tcggcgctct gctcgtcgat gctgacgatg
      601 acgccgcagg tgttgccgtc gaagccgtag ttggcgttgt cgtagccgat gtccttgatg
      661 acctcgcggg cgatcttggg gatgtcgaca taggcattgg tcgtgatctc gcccgccacc
      721 acgcacaagc cggtcgtcag cagcgtttcg caggcgacgc gaccgttggg atcgtcggtc
      781 aggatcgcgt cgaggacggc atcgctgacc tgatcggcca tcttgtcggg gtggccttcg
      841 gtcacgctct cgctggtgaa agtccaggtg ctcactggga tgctccgttg cggttgggac
      901 gccggatcgc ggcgcggtgg aggacgatcg ctgtactcgt ccgggcagac acgacgatgc
      961 cggtcatggt tgtacccggc cggcgcggat ttcgccaaca cggtcgagta cggcccgcgc
     1021 gatcgagcgc ttgtcggcca ggctgaccac caccggggcg gcatgcggcg ccagcagtgt
     1081 gacctcgttg gtgtcgtgct ggaacccgac gttggccgcg ccgacgtcgt tggccacgat
     1141 caggtcgagg ttcttgcggg tcagcttggc agtggcgttg gcgaccagat cgtcggtctc
     1201 ggcggcgaag ccgaccagca cctggccctg cggcttgacc gcgccgagcc cggcgaggat
     1261 gtcgggggtc ggttcgagca cgatctgggg tggcccgtcg tgcttcttga gcttgttcgg
     1321 cgcagccgcg acgggacgga agtcggccac cgcggcagcc atcacgacca caccggcgtt
     1381 gggagccaac cgctcgacgg cggcctgcat ctgcgctgcg gtctcgaccc gctcgacgcg
     1441 cacgcccacc ggcacaggca gatcgacggt ggagatcagc gtgacctgcg cgccgcgggc
     1501 ggcggcctcg gcggcgatcg cgtacccctg cttgccgctg ctgcggttgg cgacgacgcg
     1561 cacggcgtcg atcggctcgc gggtaccacc agcggtgacg ac
//
LOCUS       DAORNF010000791         1594 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_54675_length_1594_cov_1.831059, whole genome shotgun sequence.
ACCESSION   DAORNF010000791 DAORNF010000000
VERSION     DAORNF010000791.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54675_length_1594_cov_1.831059"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    211..360
                     /estimated_length=150
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <361..591
                     /locus_tag="PLV68_21460"
     CDS             <361..591
                     /locus_tag="PLV68_21460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03878.1"
                     /translation="AVTVDEAGGTVVDLTVTVAAASLGLTGDATVPLAVRIDDGVLSR
                     VTYSTTTSDGRTAVATTVIGPVVDGSPVVAPA"
     assembly_gap    593..602
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            707..1261
                     /locus_tag="PLV68_21465"
     CDS             707..1261
                     /locus_tag="PLV68_21465"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HRE03879.1"
                     /translation="MNADAIVEVRPTDPLEPIFRQHYARLVRTLTLLAGDRDLAADAV
                     QDAFVKAHLNWWRIKRYDDPVGWIRRVAINRLRDRHRSSAREQRAITRLIATTTGESS
                     EGTEPVDPAGSGASDLDIQQLLAGLPRQQRIAIALFYVEGLSLAEVAATMRLSVGAVK
                     YHVHQGRARLREALADESEESPDA"
     gene            1254..>1594
                     /locus_tag="PLV68_21470"
     CDS             1254..>1594
                     /locus_tag="PLV68_21470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03880.1"
                     /translation="MPEHFPDHPAGRDPGDDVTRHLVAASGDLPDTNAALAVFRRRLA
                     VARRRRRGGAIVACGAVLVAATMVVASWRGETSDNFVTDATGAVDDTAAVADSGPDDA
                     TTSQPTSTTAVP"
BASE COUNT          225 a          537 c          457 g          215 t
ORIGIN      
        1 accaccatcg ccacggtcac gacgaacgcg ccccctaccg ccggtaccga agcactgcct
       61 gatacgcctg aaccgacgac tgtcccgatc gatggtgcgt cagtgttggc agcttcgatg
      121 gcgtcgatcg cgaccagcta ccactaccac tcggtcgtca ccgtcgacgg cgtcgagacg
      181 ttggtggtcg acggtgaccg cgttggcgac nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 gcggtcaccg tcgacgaggc tggcggaacc gtcgtcgacc tgaccgtgac cgtcgccgcc
      421 gccagcctcg ggttgaccgg agacgccacg gtgcccctcg cggtccgcat cgacgacggg
      481 gtgctcagcc gcgtcaccta cagcaccacg accagcgatg ggcgcaccgc tgtcgccacc
      541 accgtgatcg ggcctgtcgt cgacggcagt ccggtggtgg caccggcctg agnnnnnnnn
      601 nnccgaccac gacctcgtcc cgctcgtctg tcgcaccgcg tagcgtgggc ggtggcccaa
      661 accatccacc gccgccaggc gtgtcttgtc tgtggagatc ttcgcggtga acgccgacgc
      721 catcgttgag gttcgcccca ccgatccctt ggaaccgatc ttccgccagc actacgcgcg
      781 cttggtgcgc accctgacgc tgctcgctgg cgatcgcgac ctcgccgccg atgccgtgca
      841 ggacgctttc gtcaaggcgc acctcaactg gtggcgcatc aagcgctacg acgacccagt
      901 cggctggatt cgccgggtgg ccatcaatcg tctgcgcgac aggcatcgca gctcggcccg
      961 cgagcagcgc gcgatcaccc ggctcatcgc cacgaccacc ggcgagtcgt ccgaaggcac
     1021 agagccggtc gaccccgccg gcagcggtgc ctccgacctc gacatccagc aactgctcgc
     1081 cggcctcccc cgccaacagc gcatcgccat cgcgctgttc tacgtggaag gtctgtcgct
     1141 tgctgaagtc gcggcaacga tgcgtctcag cgtcggtgcc gtgaaatatc acgtccacca
     1201 gggccgggcg cggcttcgtg aagcccttgc cgacgagtcc gaggagagcc ccgatgcctg
     1261 aacacttccc tgaccatcca gccggccgcg acccgggcga cgacgtcaca cgacacctcg
     1321 tcgcggccag cggcgatctg cccgacacca atgccgcgct ggccgtgttc cgccgccggc
     1381 tcgccgtagc gcggcggcgg cgccggggcg gcgcgatcgt ggcgtgtggt gccgtgctgg
     1441 tcgcggcgac gatggtcgtc gcgtcctggc gaggcgagac gagcgacaac ttcgtcacag
     1501 acgcgaccgg tgccgtcgac gacaccgcgg cagtcgccga cagtggtcct gacgacgcga
     1561 cgacgtccca accgacgagc accaccgccg tgcc
//
LOCUS       DAORNF010000792         1591 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_54812_length_1591_cov_1.671224, whole genome shotgun sequence.
ACCESSION   DAORNF010000792 DAORNF010000000
VERSION     DAORNF010000792.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54812_length_1591_cov_1.671224"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            358..>1591
                     /locus_tag="PLV68_21475"
     CDS             358..>1591
                     /locus_tag="PLV68_21475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HRE03881.1"
                     /translation="MVVRFAGDSGDGMQLTGDRFTSSSAIFGNDLSTLPDYPAEIRAP
                     AGTVMGVSSFQVHISDHDITTPGDSPNVLIAMNPAALKADLGRLEQGGLLIVNRDAFT
                     ERDLEKVDYATNPLEDGSLNNYRVVEVPMTSLTIEATKELGVKSRDAERSKNFFALGL
                     VSWLFSRPREPTLGWIGGRFKDDLIRASNTAAFNAGFHFGETTELFDHAYQVESANLA
                     AGEYTNIAGNTALAWGLIAAAQLAKLPLFLGSYPITPASDILHELSKNKHLGVRTVQA
                     EDEIAAAGMALGAAFAGHLGITTTSGPGVALKSETISLGVSLELPLLLVDIQRGGPST
                     GLPTKTEAADLNIAMFGRHGEAPLPIVAAYSPSHCFHAAIEAARIAVTYRTPVILLSD
                     GYIANGTEPWRLPDVDDLP"
BASE COUNT          268 a          552 c          516 g          255 t
ORIGIN      
        1 tcggtggggt cgatgccgag cacccaggtg ctcgccgctt gcgggtcgag gtcgaccacc
       61 agcacggtct gtccggcggc cgcggcggca ctggccagac caagggtcac cgtggtcttg
      121 ccgacgccac ctttctgatt gagcagggca acgatgcgag ccatggcgac atgttggcac
      181 gccgggcaga agggcgcttc gccgtgtgta tttcgggtcg tttgtcaccg tgtggcaggt
      241 tgtgctgggt gtaagttcca ggtattgcgg gcggaggccc gtgaccgggg caacggcgcc
      301 ccatcccaca ggaggtcacc atgaccgaca aagccgcagg ctgagacgat cagcagagtg
      361 gtcgtccgct tcgcgggcga ctccggcgac ggcatgcagc tcaccggcga tcggttcacg
      421 tcgtccagcg ccatcttcgg caacgacctg tcgacgctgc ccgactaccc cgccgagatc
      481 cgcgccccag cgggcacggt aatgggcgtc tcgagctttc aggttcacat ctccgaccac
      541 gacatcacca cgccgggtga ctcgcccaac gtgctgatcg cgatgaaccc ggctgcgctc
      601 aaagccgatc tcggccggtt ggagcagggc gggctgctga tcgtcaaccg cgacgcgttc
      661 accgaacgcg atctcgagaa ggtcgactac gccaccaacc cgctcgagga cggctcgctc
      721 aacaactacc gcgtggtcga ggtgccgatg acctcgctga cgatcgaggc caccaaggag
      781 ttgggggtca agagccgcga tgccgagcga tcgaagaact tcttcgccct ggggctcgtc
      841 tcgtggttgt tcagccgccc gcgcgaacca acgctcggtt ggatcggtgg gcggttcaag
      901 gacgacctca tccgcgcctc caacacggcc gcgttcaatg ccgggttcca cttcggtgag
      961 acgaccgagt tgttcgacca cgcctatcag gtcgagtcgg ccaacctggc cgcgggggag
     1021 tacaccaaca tcgccggcaa caccgcgttg gcgtggggcc tcatcgccgc cgcgcagctc
     1081 gccaagctgc cgctgttcct gggcagctac ccgatcaccc cggcgtccga catcctccac
     1141 gagctctcca agaacaagca cctcggtgtg cgcacggtgc aagccgagga cgagatcgcg
     1201 gcggcgggca tggcgctcgg tgcggccttc gccggccatc tcggcatcac caccaccagt
     1261 gggccgggcg tcgcgttgaa gagcgagacg atcagcctcg gtgtgtcgct ggagctgccg
     1321 ctgctgctgg tcgacatcca gcgcggcggt ccgtccaccg gcctgccgac caagaccgaa
     1381 gcggccgacc tcaacatcgc gatgttcggt cgccacggcg aagcgccgct gccgatcgtg
     1441 gccgcgtaca gccccagcca ctgtttccac gccgcgatcg aggcggcccg cattgcggtc
     1501 acctaccgca cgccggtgat cctgctcagc gacggctaca tcgccaacgg caccgagccg
     1561 tggcgattgc ccgacgtcga cgacctgccc g
//
LOCUS       DAORNF010000793         1586 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_55009_length_1586_cov_1.755062, whole genome shotgun sequence.
ACCESSION   DAORNF010000793 DAORNF010000000
VERSION     DAORNF010000793.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55009_length_1586_cov_1.755062"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>240)
                     /locus_tag="PLV68_21480"
     CDS             complement(<1..>240)
                     /locus_tag="PLV68_21480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03882.1"
                     /translation="VRAAVQEDVQGVAISSYQGGHVEYFTYLLERLRDEGVGHVRVYG
                     GGGGVIVPAEIELLHQRGVARLFSPHDGQRMGLAQM"
     assembly_gap    241..414
                     /estimated_length=174
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            547..>1586
                     /locus_tag="PLV68_21485"
     CDS             547..>1586
                     /locus_tag="PLV68_21485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03883.1"
                     /translation="MRSVLINQAANASRRSATHRWRTVVTSAAVATVTALAWGGVAEA
                     APAATVAPRPANEVPAETTITLPLFGAPLTIDVTAAPGGAISSVSIDPADGFVASVAK
                     PKKVVFTDSANGASVSVSTKHGGEKVQARAGSLTDILGPGTWSGDLFGTGSSTTVSFE
                     IAAADDGGPTITNVTTSDPGAVIGDLQRHTGEKGWGWNDKGGTFSSASVQIGFTDGLQ
                     SRRLVIRASTGNRGEQTSAKVSVSLGDIRGRVVELAEAVGPHTWTGTLCNGSTASITY
                     AVADDGTLTAVTADPATATVTLDRDATGAKITFSATEAVRIKVKAKGDGGTVRVSVDE
                     RIRCEAGTPVVNT"
BASE COUNT          251 a          509 c          443 g          209 t
ORIGIN      
        1 catctgcgcc agacccatcc gctggccgtc gtggggcgag aacagccggg ccacaccacg
       61 ctgatgcagc agttcgattt cggccggcac gatcacgccg ccgccgccgc catagacccg
      121 cacgtgaccg acgccctcgt cacgcaggcg ttcgagcagg taggtgaagt actcgacgtg
      181 gccgccctgg tacgagctga tcgcgacacc ctgcacgtcc tcctgcaccg cggcgcgcac
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnccatat
      421 tacttagacg tcaaagtatc tggccagccc ggttcgtccc cgaccgtcca gccctggcca
      481 aacctttcgg gggtcgtgcg gcgtgtcaca ttccagcggg tgtaccaatt catcgaggag
      541 agatccatga gaagcgtgct catcaaccag gcggccaacg cctccaggag gtcagcgaca
      601 caccgttggc gcaccgtggt cacgtcagcc gccgtcgcga ctgtcaccgc cttggcctgg
      661 ggtggagtcg cggaagcggc acccgcggcc acggtcgcac cgcgaccggc caacgaggtg
      721 ccggccgaga ccacgatcac actgcccttg ttcggcgctc cactgaccat cgacgtcacc
      781 gccgcaccag gtggcgcgat ctccagcgtg tcgatcgatc cggctgacgg ttttgtcgcc
      841 agcgtcgcca aacccaagaa ggtcgtgttc accgactccg cgaacggtgc gtccgtctcg
      901 gtgtcgacca agcacggcgg cgaaaaggtc caagcccggg ccggcagcct gaccgacatc
      961 ctcggccccg gtacctggag cggcgacctg ttcggcaccg gctcctccac cacggtcagc
     1021 ttcgagatcg cagccgccga cgacggtggg cccacgatca ccaacgtcac gacctccgat
     1081 cccggggcgg tgatcggtga tctccaacgc cacaccggtg agaagggctg gggttggaac
     1141 gacaagggcg gcacgttctc gtcggcgtcg gtgcagatcg ggttcaccga cggtctccag
     1201 tcgcgtcgtc tggtcatccg cgccagcacc ggaaaccgcg gcgagcagac ctccgccaag
     1261 gtgtcggtca gccttggtga catccgcggc cgagtcgtcg agctggcaga agctgtcggt
     1321 cctcacacct ggaccggcac gctgtgcaac ggttcgacag cctcgatcac ctacgccgtg
     1381 gccgacgacg gcaccctgac cgcagtgacc gccgaccctg cgaccgcaac ggtcacgctc
     1441 gaccgcgacg ccaccggcgc caagatcacc ttctcggcca ccgaggcagt gcggatcaag
     1501 gtcaaggcga agggcgatgg cggcacggtg cgggtctcgg tcgacgagcg catccgctgc
     1561 gaggccggaa cccccgtggt caacac
//
LOCUS       DAORNF010000794         1586 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_55017_length_1586_cov_1.568909, whole genome shotgun sequence.
ACCESSION   DAORNF010000794 DAORNF010000000
VERSION     DAORNF010000794.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55017_length_1586_cov_1.568909"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>840)
                     /locus_tag="PLV68_21490"
     CDS             complement(<1..>840)
                     /locus_tag="PLV68_21490"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRE03884.1"
                     /translation="PNAGSDLASLKTSAELDGEEWIINGQKVWTTQGHHADYCFLLTR
                     TDPDAPKHKGISYLLVPMRQPGVEVRGIVQPDGTAEFCEVFFDNARCPKDNVVGGVNN
                     GWKVANSTLAFERGMSATTGYRRFEDEYRNMVATATANGAIDDATVRQRLMRYYTKIL
                     ILRVNGLRSLSATLSGTKDLGVAALGATNKMFWSEMHKEAMELALDIYGAEAMLIETG
                     PASWPGTGRDKRREGYPVSPMMSAFFFSRSETIWGGTSQIQRNIVGERVLGLPKEPSA
                     EAR"
     assembly_gap    841..961
                     /estimated_length=121
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<962..1228)
                     /locus_tag="PLV68_21495"
     CDS             complement(<962..1228)
                     /locus_tag="PLV68_21495"
                     /inference="COORDINATES: protein motif:HMM:NF014790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRE03885.1"
                     /translation="MDLSYADEQFRTEIRAWLEAHLPQGWFEPGFEMTPEQRKAFNES
                     WPTTLFEGGWICATWPTEYGGKGLTTMQGVVLAEEFARAKAPMRA"
     gene            1342..1497
                     /locus_tag="PLV68_21500"
     CDS             1342..1497
                     /locus_tag="PLV68_21500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03886.1"
                     /translation="MAIVIAFIVLALIFGVGGLVKGLFWLASIGLILLLVAIALAIST
                     TRRSGSS"
BASE COUNT          218 a          483 c          484 g          280 t
ORIGIN      
        1 agcgagcttc ggcgctgggt tccttgggca gaccgagcac gcgctcgccg acgatgttgc
       61 gctggatctg gctggtacca ccccagatcg tctcgctgcg gctgaagaag aaggcgctca
      121 tcatcgggct gaccgggtac ccctcgcggc gcttgtcgcg cccggtgccg ggccatgacg
      181 cggggccggt ctcgatcagc atcgcctcgg cgccgtagat gtcgagagcc aactccatcg
      241 cttccttgtg catctccgac cagaacatct tgttcgtcgc accgagtgcc gcgacaccca
      301 ggtccttggt gccactgagg gtcgcgctga gcgagcgcag gccgttgacg cgcaggatga
      361 ggatcttggt gtagtagcgc atcaggcgct ggcgcaccgt ggcgtcgtcg atggcgccat
      421 tggcggtcgc ggtggcgacc atgttgcggt attcgtcctc gaagcggcgg tagccggtgg
      481 tggcgctcat gccccgctcg aacgcgagcg tggagttggc caccttccag ccgttgttga
      541 cgccgccgac cacgttgtcc ttggggcaac gagcgttgtc gaagaacacc tcgcagaact
      601 cggccgtacc gtcgggctgg acgatgccgc gcacctcgac ccccggctgg cgcatgggta
      661 ccaacaggta ggagatgccc ttgtgcttgg gcgcgtcggg gtcggtgcgg gtgagcagga
      721 agcagtagtc ggcgtggtga ccctgcgtgg tccacacctt ctggccgttg atgatccact
      781 cctcgccgtc gagctcggcc gaggtcttga ggctggccag gtcggagccg gcgttgggct
      841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      961 nggcccgcat cggggccttg gcccgggcga actcctcggc cagcaccacg ccttgcatcg
     1021 tggtcagacc cttgccgccg tactcggtgg gccaggtggc gcagatccag ccaccttcga
     1081 acagcgtcgt cggccacgac tcgttgaacg ccttgcgttg ttcgggggtc atttcgaagc
     1141 ccggctcgaa ccagccctgc ggaaggtgcg cttccaacca ggcccggatt tcggtgcgga
     1201 actgctcgtc agcgtagctc aagtccatgc cgggcagtct gcctgcccgc aaccccccac
     1261 acagaaccgg cgcggcgtga atcgcgccac cgcgacgaag cggcgagtct gaacaccacc
     1321 accgcggggt acggggtgtc catggccatc gtcatcgcgt tcatcgtcct cgccctcatc
     1381 ttcggtgtcg gcgggttggt caaaggcctg ttctggttgg cttcgatcgg gctgatcctg
     1441 ctgctcgtcg cgatcgcgct cgccatctcc accacccgcc gcagcggcag ttcgtagcgt
     1501 cagctccgga tcgtcaagga tccaacgggc catgcttgac aaaccactct ccgtgagctg
     1561 ctttcactca gcgtgagatc ggtgta
//
LOCUS       DAORNF010000795         1585 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_55063_length_1585_cov_1.633987, whole genome shotgun sequence.
ACCESSION   DAORNF010000795 DAORNF010000000
VERSION     DAORNF010000795.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55063_length_1585_cov_1.633987"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            125..460
                     /locus_tag="PLV68_21505"
     CDS             125..460
                     /locus_tag="PLV68_21505"
                     /inference="COORDINATES: protein motif:HMM:NF022684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3040 domain-containing protein"
                     /protein_id="HRE03887.1"
                     /translation="MPLSEEEQRILRQIEEQLQADPAFAMKVNTRPGRRSTSQGRLAW
                     SAVALVAALVGVIAALPISAWLSFAAFIGMVAAGAVLERELSARGRDQLSRIPEELRR
                     RSGRSTNPS"
     gene            complement(520..>714)
                     /locus_tag="PLV68_21510"
     CDS             complement(520..>714)
                     /locus_tag="PLV68_21510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03888.1"
                     /translation="GPHTMRELAGAATAAIYGERGDPSTALHCADLSEQIVTSVKRRQ
                     SLPQRILNLIDPRRVAQLQP"
     assembly_gap    715..839
                     /estimated_length=125
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<840..>1585)
                     /locus_tag="PLV68_21515"
     CDS             complement(<840..>1585)
                     /locus_tag="PLV68_21515"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HRE03889.1"
                     /translation="ATSSNPPDPLTLDLPSGFPGEVTATALAVTADAPNNYEKLLALQ
                     RWFHSEFTYDLTVQRGHSDNAMLNFLRVRRGYCEQFAGTFAAMARAIGIPSRVAVGYT
                     PGELLADGKFHVYGRNAHAWPEVWFDDIGWVSFEPTPDGFASPSVGVPAPTGRPRRRF
                     GRHDHGHHRTADVDHRRQRPVGADHHHRSQRGRPHRHHPAARRGQYVGVLLQQHGLVR
                     VAGDRGRDRLAHADAAGDRCLAPPRHQPRA"
BASE COUNT          208 a          454 c          563 g          235 t
ORIGIN      
        1 tgcgcgccgt gcatcccgtg ctcgcgccgc gttgtcagcc actggccgac gtgagaaact
       61 gtcggcgacc cgacgcccgt cggtcggatg acccccatcg gtcggaagtc ccgggagaac
      121 cctgatgccg ctctccgaag aagaacagag gatcctccgc cagatcgagg agcaactcca
      181 ggccgacccg gcattcgcga tgaaggtcaa cacacgcccg ggacgacgca gcacctcgca
      241 gggccgcctg gcctggagcg cagtcgcgtt ggtcgccgcg ctcgttggcg tgatcgccgc
      301 attgccgatc agcgcctggt tgtcgttcgc ggccttcatc ggcatggtcg ccgccggtgc
      361 cgtgctcgaa cgcgagctgt ccgcccgcgg tcgcgatcag ctcagccgca tccccgaaga
      421 gctccgccgc cgctctggcc gcagcaccaa cccgagctga tccgttcgcc ggatgcggcg
      481 gcccagctca gggttggtaa cagtcagggt tggtaacagt cagggttgga gttgggcgac
      541 gcggcggggg tcgatcaggt tgaggatgcg ttgggggagt gactggcggc gcttgaccga
      601 ggtgacgatc tgctcgctca ggtcggcgca gtgcagcgct gtcgatggat cgccgcgctc
      661 gccgtagatg gcggcggtgg cggcacctgc cagctcgcgc atggtgtggg ggccnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng
      841 gctcgtggct ggtgtcgcgg cggcgccagg cagcgatcgc ccgcggcatc agcgtgagcc
      901 aggcgatcac ggccgcgatc gccagcaaca cgaaccagac cgtgttgctg gaggaggact
      961 ccgacgtact ggccgcggcg ggcggctggg tggtggcggt ggggccttcc gcgctgcgat
     1021 cgatggtggt ggtcggcgcc gacgggtcgt tggcgacggt ggtcgacgtc ggcggtgcgg
     1081 tggtggccgt ggtcgtgtcg tccgaatcgt cgccgggggc ggcctgttgg ggcgggcacg
     1141 ccgacggaag gtgatgcgaa gccgtcgggg gtcggctcga agctgaccca accgatgtcg
     1201 tcgaaccaca cctcgggcca ggcgtgtgcg ttgcggccgt agacgtggaa cttgccgtcg
     1261 gccaacagct cacccggggt gtacccgacc gcgacccgcg aggggatgcc gatcgcgcgg
     1321 gccatcgccg cgaacgtacc ggcgaactgc tcgcagtagc cgcgccggac ccggaggaag
     1381 ttgagcattg cgttgtcgct gtgaccccgc tgcacggtga ggtcgtaggt gaactcgctg
     1441 tggaaccacc tctgcaaggc gagcagcttc tcgtagttgt tgggtgcgtc ggcggtgacg
     1501 gccagcgcgg ttgcggtgac ctcgccgggg aagccgctgg gcagatcgag ggtgagcggg
     1561 tcgggcggat tggacgaggt cgcgg
//
LOCUS       DAORNF010000796         1584 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_55104_length_1584_cov_1.744931, whole genome shotgun sequence.
ACCESSION   DAORNF010000796 DAORNF010000000
VERSION     DAORNF010000796.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55104_length_1584_cov_1.744931"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>771)
                     /locus_tag="PLV68_21520"
     CDS             complement(<1..>771)
                     /locus_tag="PLV68_21520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HRE03890.1"
                     /translation="LSAHGSAPEVVAAARHRGSYVVDSVCPLVTKVHHEVKVRAGKGY
                     RIVYVGHEGHEEAVGTMAVAPTAISRVESVAEVEALPHFDEPVALLAQTTLSHRDWEG
                     VAVAVRDRYPDVWSPGRSDLCFATTNRQSALMNIAPRVDALIVIGSANSSNTRALEKL
                     AIEAGCPRVFRINSVDELPNDLHGSIGVTAGASAPEDLVQAVLDHLAPRHGVETVRTV
                     DEHEYFPPPRNIRDLQTAISRAAAIMLGAPAEAATVDDR"
     assembly_gap    772..838
                     /estimated_length=67
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<839..925)
                     /locus_tag="PLV68_21525"
     CDS             complement(<839..925)
                     /locus_tag="PLV68_21525"
                     /inference="COORDINATES: protein motif:HMM:NF014456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03891.1"
                     /translation="MAIKALAFMVRSFPPPVYCYHEIVHNKII"
     gene            1020..>1584
                     /locus_tag="PLV68_21530"
     CDS             1020..>1584
                     /locus_tag="PLV68_21530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HRE03892.1"
                     /translation="MSLPTIARVVPGSPADLAGIRAGDEVVTMNGQTPRDVIEWQFLA
                     DEAEVDFELRRGNIELQVDVVKADGEPLGLDVESAVFDRVRTCDNHCEFCFIYQLPKG
                     MRRSLYLKDDDYRLSFLYGNFTTLTRFTEADLERVITERLSPLHVSIHATDAETRVRM
                     LRNPRGAMSLRWLRALLDHGITVHGQIV"
BASE COUNT          248 a          495 c          531 g          243 t
ORIGIN      
        1 cggtcgtcga cggtggccgc ttcggcgggg gcgccgagca tgatcgccgc ggcgcggctg
       61 atcgcggttt gcaggtcgcg gatgttgcgc ggcggcggga agtactcgtg ctcgtcgacg
      121 gtgcgcaccg tctcgacccc gtggcgggga gcgagatggt cgagcactgc ttgcaccagg
      181 tcttcggggg ccgaagcacc agcggtgacg ccgatgctgc cgtgtaggtc gttgggaagc
      241 tcgtcgacgc tgttgatgcg gaacacgcgc gggcagccag cttcgatggc gagcttctcc
      301 aaggcgcggg tattggacga gttggccgag ccgatcacga tcagcgcatc gacccgtggc
      361 gcgatgttca tcaatgccga ctggcggttg gtcgtggcaa agcacaagtc gctgcgcccc
      421 ggcgaccaga cgtcggggta gcgatcgcgc acggcgacgg cgacgccttc ccagtcgcga
      481 tgcgacagcg tggtctgggc cagcaaggcg accggctcgt cgaagtgggg gagcgcttcg
      541 acctcggcga ccgactcgac ccggctgatc gccgtggggg cgacggccat ggtcccgacc
      601 gcctcctcat ggccttcgtg gccgacgtag acgatgcggt agcccttgcc ggcgcggacc
      661 ttgacctcgt ggtgcacctt ggtgaccagc gggcagaccg aatcgaccac gtagctgccg
      721 cgatgacggg ctgcggcgac cacctcgggc gccgagccat gagcggagag cnnnnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnga
      841 tgatcttgtt gtgcacgatc tcgtggtagc agtacaccgg cggcgggaag ctgcggacca
      901 tgaacgccaa ggccttgatc gccatctcga ccccggcgca gaacccccgc ggctcggcga
      961 gcagcacgcg gtcgacatcc atggggccac gctaccgatg ccagtagcct tgacgggcta
     1021 tgtccctgcc caccatcgcc cgtgtcgtac cgggatcgcc ggccgacctg gccggcatcc
     1081 gcgccggcga cgaggtggtc acgatgaacg gtcagacgcc gcgcgacgtg atcgagtggc
     1141 agttcctggc cgacgaggca gaggtcgact tcgagttgcg tcgcggcaat atcgagctgc
     1201 aggtcgacgt cgtcaaagcc gacggtgaac cgctcgggct cgacgtcgag agcgcggtct
     1261 tcgaccgggt gcgcacctgc gacaaccatt gcgagttctg cttcatctac cagttgccca
     1321 agggcatgcg ccgcagcctg tacctcaaag acgacgacta ccggctcagc ttcctgtacg
     1381 gcaacttcac cacgctgaca cgcttcaccg aggccgatct ggaacgggtg atcaccgagc
     1441 ggctgtcacc gctgcacgtc agcatccacg ccaccgacgc cgagacccgg gtgcggatgc
     1501 tgcgcaaccc gcggggtgcg atgagtctgc gctggctgcg ggcgttgctc gaccacggca
     1561 tcaccgtgca cggccagatc gtgg
//
LOCUS       DAORNF010000797         1579 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_55317_length_1579_cov_1.770341, whole genome shotgun sequence.
ACCESSION   DAORNF010000797 DAORNF010000000
VERSION     DAORNF010000797.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55317_length_1579_cov_1.770341"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..550)
                     /locus_tag="PLV68_21535"
     CDS             complement(<1..550)
                     /locus_tag="PLV68_21535"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRE03893.1"
                     /translation="MAYANSEGARIYYEETGSGTPILFLHEFAGDFRSWADQIRHLAR
                     GWRCITMSARGYPPSDVPAEESAYGQDIANRDAIAVLDHLKIDRAHVVGLSMGAYTAL
                     QLAVHFPERVRAAVPCGGGSGSYPPQRAQFIRDSIDRAAAFEKAGKIPAEAMGLGPTR
                     VQLLNKDPIGWRIATEHQASHPA"
     gene            complement(566..>1579)
                     /locus_tag="PLV68_21540"
     CDS             complement(566..>1579)
                     /locus_tag="PLV68_21540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03894.1"
                     /translation="NTFDLNTARPAGEYYVVVRSASGQKTSYTMTVQVRPAEPYTHHE
                     IPYKMAVGTGTNEFKTLVTNVLQSRFVEFRVPSAAIYKITLAYGQAESGTDVDIILFR
                     DTSGNGSWQKVTDSSRDPGVDEQIVHSLEPGRYLIRMLWDRSSRGKSANVTLAISQTT
                     EMVEPTPYGPVLGRHGDWMVTTVTRDNVKFCYAYTLATSVSPSGWRAIRPRLALRVRE
                     NNNGLFHTFDVVGPYNKSRGWEATIRGRTGTFKIPVSVFKDENDIQSQVPCTNDRSNM
                     CVSNDGMRGLTRGQELTLTGTSSDGRSVSIIYSLNGYQAAIREMNKTCNNERNTGWLI
                     QTN"
BASE COUNT          248 a          494 c          537 g          300 t
ORIGIN      
        1 aggccgggtg cgaggcctgg tgctcggtcg cgatgcgcca gccgataggg tctttgttga
       61 gaagctgtac gcgcgtcgga cccaggccca tcgcctcggc tggaatcttc ccggccttct
      121 cgaaggcggc cgcgcggtcg atcgaatcgc ggatgaactg cgcgcgctgc gggggatagg
      181 agcccgaccc gccgccgcac ggcaccgcag cccgcacacg ctcggggaaa tgcactgcca
      241 actgcagcgc cgtgtaggcg cccatgctga gacctaccac gtgcgcgcga tcgatcttca
      301 gatgatcgag gacggcgatg gcgtcgcggt tggcgatgtc ctggccgtag gcgctctcct
      361 ctgccggtac gtccgagggc gggtagccgc gcgccgacat ggtgatgcag cgccagccgc
      421 gggcgaggtg gcggatctgg tcggcccaac tccggaagtc gccggcgaac tcgtggagga
      481 acaggatcgg cgttcccgac cccgtctctt cgtagtagat gcgtgcacct tcggagttgg
      541 catatgccat ggcaacctcg tcccgtcagt tggtctggat cagccagccc gtgttgcgct
      601 cgttgttgca ggtcttgttc atctcgcgga tggccgcctg atagccgttg agcgagtaga
      661 tgatggagac actgcgtcca tcgctcgacg tgccggtgag cgtcagctcc tgcccgcgcg
      721 tcagcccgcg catgccgtcg ttggagacgc acatgttgga acggtcgttg gtgcagggca
      781 cttgcgactg gatatcgttc tcatccttga acacgctgac cgggatcttg aaggtaccgg
      841 tgcgaccgcg gatggtcgct tcccagccgc gcgacttgtt gtaagggccg accacgtcga
      901 aggtgtggaa gaggccgttg ttgttctcgc ggaccctgag tgccagacgc ggccggatgg
      961 cccgccagcc ggacggcgag accgaggtcg ccagcgtgta ggcgtagcag aacttcacgt
     1021 tgtcgcgcgt cacggtcgtc accatccagt cgccgtgccg gccgagcacg ggaccgtaag
     1081 gcgtcggctc caccatctcg gtcgtctgcg agatggcaag agtgacgttc gccgatttgc
     1141 cacggctcga gcggtcccag agcatgcgga tcagatagcg gccgggctcc agcgagtgca
     1201 cgatctgctc gtcgacgccg ggatcgcgcg agctgtcggt caccttttgc cagctgccgt
     1261 tgccgctcgt gtcgcggaac aggatgatgt cgacgtccgt gcccgactcg gcctggccat
     1321 aggcaagcgt gatcttgtag atcgccgcgc tcggcacgcg gaactcgacg aagcgcgact
     1381 gcagcacgtt ggtgacgagc gtcttgaact cgttggtgcc cgttccgacc gccatcttat
     1441 aggggatctc gtggtgcgtg tagggctcgg cgggacgcac ctgcaccgtc atcgtgtacg
     1501 aggtcttctg cccgctcgca ctgcgcacga cgacatagta ctcgcccgcc ggccgcgccg
     1561 tattgagatc gaatgtatt
//
LOCUS       DAORNF010000798         1579 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_55318_length_1579_cov_1.721785, whole genome shotgun sequence.
ACCESSION   DAORNF010000798 DAORNF010000000
VERSION     DAORNF010000798.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55318_length_1579_cov_1.721785"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /locus_tag="PLV68_21545"
     CDS             <1..585
                     /locus_tag="PLV68_21545"
                     /inference="COORDINATES: protein motif:HMM:NF028519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HRE03895.1"
                     /translation="DGDGIGDNADTDDDGDGVQDASEAPLPGVTVDLLGPTGNLIATM
                     PTDANGRYTFDNLPAGTYQVRFTLTPAQAAMYDFTTRDQGGNDANDSDADTMTGLTRT
                     FVLDDTNTALTKTYDRTVNATQGIDPTWDAGVVLRESAPPDTTTPAPTTTVPGGALPV
                     LGSSVGTMLMVAFMLVFGGLVAIATRRRSARPNA"
     gene            738..1445
                     /locus_tag="PLV68_21550"
     CDS             738..1445
                     /locus_tag="PLV68_21550"
                     /inference="COORDINATES: protein motif:HMM:NF012879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRE03896.1"
                     /translation="MTALDVEQLHITRLANGLTVATECIPSALSVSAGAWVGVGARDE
                     TAEMAGVSHFLEHLLFKGSDAHSAREISQMVDRVGGDINAFTAKEYTAYYTRVPARHQ
                     AMAIELLGEVLTRPALRPDDVDNERQVILEERNQRTDNNPESLFWEQLLATLYVAHPS
                     TRPRSSRDGRDRRGDHRGRHPPVLRGALPQRQHGGGGVRPDRPRRDGGRDRPRLRGHG
                     PRRRPRRSRPLQRRTRW"
BASE COUNT          267 a          559 c          516 g          237 t
ORIGIN      
        1 gatggtgatg gcattggcga caatgctgac accgacgacg atggtgatgg cgtgcaggat
       61 gcgagcgaag cgccgttgcc tggcgtgacc gtcgacctgc tcggcccgac cggcaacctg
      121 atcgcgacca tgccgaccga tgccaacggc cggtacacgt tcgacaacct gccggcggga
      181 acctaccagg tgcgcttcac cctgaccccg gcgcaggctg cgatgtacga cttcaccacc
      241 cgtgaccagg gcggcaacga cgccaacgat tcggatgccg acacgatgac cgggctgacc
      301 cgcacattcg tgctcgacga caccaacacg gcgctgacga agacctacga ccgcacggtc
      361 aacgccacgc agggcatcga tcccacctgg gatgccggcg tggtcctgcg tgagtcggcg
      421 ccgccggaca cgaccactcc ggcccccacg acgacggtgc ccggtggcgc tctgccggtg
      481 ctcggctcgt cggtcggcac catgctgatg gtggcgttca tgctcgtgtt cggtggcctc
      541 gtcgccatcg caacccgtcg ccgttccgcc cgcccgaacg cctgaggcgt ccggcaccaa
      601 ggaacgcact gtcgacgccc cctgctggga ccaccaaccg cccggtgagt cccgcagggg
      661 tgcgtcatcc ctcgtccgac gcggccactc cgaacacggt tcccatccgt gatcggcgtc
      721 catagagtgt cgccaccgtg acagcgctcg acgtcgaaca actgcacatc acccggctcg
      781 ccaacgggct caccgtggcg acggagtgca tcccgtcggc gttgtcggtg tcggccggag
      841 catgggtggg tgtcggcgct cgggacgaga cggcggagat ggcaggcgtc agccacttcc
      901 tcgaacacct gttgttcaag ggcagcgatg cccactcggc gcgcgagatc agccagatgg
      961 tcgaccgggt gggtggtgac atcaacgcct tcaccgccaa ggagtacacc gcctattaca
     1021 cccgggttcc ggcccgtcat caggcgatgg cgatcgagct gttgggcgag gtgctcaccc
     1081 gcccggcgct gcgccccgac gatgtcgaca acgagcgcca ggtgatcctg gaggagcgca
     1141 accagcgcac cgacaacaac cccgagagcc tcttctggga gcaattgctg gcgaccctct
     1201 atgtcgcaca cccatccact cggccgcgat ccagccggga cggccgagac cgtcgaggcg
     1261 atcaccgcgg tcggcatccg ccagttcttc gaggcgcact accgcagcgg caacacggtg
     1321 gtggcggtgt gcggcccgat cggccacgac gagacggtgg ccgcgatcga ccgcgccttc
     1381 gcgggcatgg cccccggcga cggccgcgtc gctcgcggcc actgcagcgc cgcactcggt
     1441 ggtgaggtga tcgaggacga caccgaacag gtccacctgg tggctggcat gcaggccctg
     1501 cgtcgcgacg accccgatcg cgaagccttc gacgtggtca accacgtgtt cggtggcggt
     1561 ctgtcgagcc gtctgttcg
//
LOCUS       DAORNF010000799         1570 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_55658_length_1570_cov_1.673267, whole genome shotgun sequence.
ACCESSION   DAORNF010000799 DAORNF010000000
VERSION     DAORNF010000799.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55658_length_1570_cov_1.673267"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..725)
                     /locus_tag="PLV68_21555"
     CDS             complement(<1..725)
                     /locus_tag="PLV68_21555"
                     /inference="COORDINATES: protein motif:HMM:NF012880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HRE03897.1"
                     /translation="MVGISEQSAGSRGPAPLLSLYREMVRGRAVEDVIVRLADERAFQ
                     GFYHPGVGQEAGPVGVCAHLRETDALFYAHRGVAYLTAKGMDPVEIIGDFLGTTAGST
                     RGLGAGTVHCVDPERGVYGQGGTLGSCFPLAAGVALALRMQGRDDVAVAFFGDGASAR
                     GTFLEAAVFAVAKALPVVFVCENNGFAISATTRESQGVDSIAGRARGFGLPAETVDGT
                     DVEAVWAAAGVAIGDVGLLIHGTT"
     gene            852..>1570
                     /locus_tag="PLV68_21560"
     CDS             852..>1570
                     /locus_tag="PLV68_21560"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRE03898.1"
                     /translation="MSASTDEIWIVSADDHVVEPPTLWTDRLPSKYADVAPRVVRDRA
                     VQTSSKGGKMAYRRSSDEGVWADWWLYEDLQIPLTRPSAASGCDHVDFSPITFDEIRP
                     GCWVQADRLADMDANRVEASICFPNTLPRFCGQTFAEAKDHDLALLCVKAYNDWVIDD
                     WCTGAATGRLIPVTIVPLWDRDAAVAEVLRCAAKGSRAMSFSENPSELGLPSIHAADG
                     YWEPLFAACAETETIINMHIGS"
BASE COUNT          270 a          556 c          491 g          253 t
ORIGIN      
        1 gtcgtgccgt gaatgagcag gccgacatcg ccgatcgcga cgccggccgc cgcccacact
       61 gcctccacat cggttccgtc gaccgtctcg gccggcaggc cgaagcccct ggcccgtccg
      121 gcgatgctgt cgactccctg gctctcgcgc gtcgtggcag agatcgcgaa tccgttgttc
      181 tcgcacacga acacgaccgg cagggccttg gcaacggcga acaccgcggc ctccaagaac
      241 gtcccccggg ccgacgcacc gtcgccgaag aacgccaccg ccacgtcgtc gcggccctgc
      301 atgcgcagcg ccagcgccac gccggccgcc agcggaaagc aactacccaa cgttccgcct
      361 tgaccgtaga cgcctcgctc gggatcgacg cagtgcacag ttccggcgcc gaggccgcgg
      421 gtgctgcctg cggtggtccc caggaagtcg ccgatgatct ccaccgggtc cattcccttg
      481 gccgtcaggt acgccacccc acggtgggcg tagaacagcg cgtcggtctc gcgcaggtgt
      541 gcacacaccc caaccggccc cgcctcctgg cccaccccgg ggtgatagaa gccttggaac
      601 gcgcgctcgt cggcgaggcg gacgatcaca tcctccacgg cccgtccgcg caccatctct
      661 cgatagaggc tgagcaacgg tgctggtccg cgcgacccag cggactgttc agagatgccg
      721 accatctcga ctcaacctat cagtgttcgg ccaagagatc agccagactt gccacctgtt
      781 gccaatcagc gtatggttat ggctccaacg agcgctgagg ggtccgtgct cggctggaag
      841 ggaagaggcc gatgtccgcg tctaccgatg agatctggat tgtctccgcc gacgaccacg
      901 tcgtcgaacc accgacgttg tggaccgacc gactgccatc caagtacgcc gacgtggcgc
      961 cacgggtcgt gcgcgaccgg gccgtgcaga cgtcgagcaa gggcggcaag atggcctacc
     1021 gccgttcgtc cgacgaaggt gtgtgggcgg attggtggct gtacgaggac ctccagattc
     1081 ccctgacgcg tccatcggcc gcatcgggct gcgaccacgt cgacttttcg ccgatcacct
     1141 tcgacgagat tcgcccaggc tgctgggtac aggccgatcg gttggccgac atggacgcga
     1201 accgggtcga ggcatcgatc tgctttccca acacgctccc ccgcttctgc ggccagacct
     1261 tcgccgaggc caaggatcac gacttggcgt tgctctgcgt caaggcttac aacgactggg
     1321 tgatcgacga ctggtgcacg ggtgcagcga ccggacgcct gatcccagtc acgatcgtgc
     1381 cgctgtggga ccgagacgcg gccgtggccg aggtgttgcg ctgtgcggcc aagggctccc
     1441 gagccatgtc cttctcggag aacccgagcg agctcggcct gccatcgatc catgccgccg
     1501 acggctattg ggaaccactg ttcgcggcct gcgccgagac cgagacgatc atcaacatgc
     1561 acatcggctc
//
LOCUS       DAORNF010000800         1567 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_55771_length_1567_cov_1.382937, whole genome shotgun sequence.
ACCESSION   DAORNF010000800 DAORNF010000000
VERSION     DAORNF010000800.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55771_length_1567_cov_1.382937"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            200..>1185
                     /locus_tag="PLV68_21565"
     CDS             200..>1185
                     /locus_tag="PLV68_21565"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HRE03899.1"
                     /translation="MVPPADAPTVSSTAGSPTPGSPTEVPPALAPRSGHRFWAGPLAG
                     VALLALTAMVLATFFTASRFTSVVRPYAIVPSDAEPVQSHLDVEGADTYPTDGSIRFV
                     TIRQPQLSLLSWLLFRDAEQVVPRTYVEVYGVSTPQQVRVRGRRQMFNAQQAAEYVAL
                     SKLGFPVERSPGAVVVDQIVCFKASDDGRSCIDQAPSGKVLQPDDEIVSVDGHEIETI
                     DDLLPVLAGHQGGDMVEVRYIRAGEDTPRTDQIELLETGAGTEDDPKRVIIGFYPFDT
                     TTISSSPFPIEFDLAGVGGPSAGLAFTLTLIDVLTPGSLTGGHEVAVTGEINV"
     assembly_gap    1186..1273
                     /estimated_length=88
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          235 a          507 c          496 g          241 t
ORIGIN      
        1 gaccgggtcg gtcgacgcga acggacgtgc ggcctctcgg gcctggcgcc ggttgacgcc
       61 ggtgtggcgc tgaccgcgtg gcaacttggg ggcggctctg gcagtctttc gccacccgag
      121 gcacaccagt gccaacatgg ccagcgctgc gatccatgca gcacggatgg tgaactcagt
      181 gaccgacacg caagaaccga tggtaccgcc ggccgatgcg cccaccgtgt cgtccactgc
      241 agggtcgcca accccagggt cgccgacaga ggtgccgcca gcgctcgctc ctcgcagcgg
      301 gcatcgcttc tgggccggcc cactggccgg tgtggccttg ctcgccttga cggcgatggt
      361 gctggccacg ttcttcaccg cgtcgcgctt cacgtcggtg gtcaggccct acgcgatcgt
      421 gccgtccgac gccgagcccg tgcagtcgca cctcgacgtc gaaggcgcgg acacgtaccc
      481 caccgacggc agcatccggt tcgtcaccat ccgtcaaccg caactgtcgc tgttgagctg
      541 gctgttgttc cgcgatgccg aacaggtcgt gccgcgcacc tacgtcgagg tctacggcgt
      601 cagcacacca cagcaggtga gggtgcgcgg ccggcggcag atgttcaacg cccagcaggc
      661 ggccgagtac gtcgcactca gcaagctggg cttcccggtc gagcgctctc cgggcgccgt
      721 ggtggtcgac cagatcgtgt gcttcaaggc atccgacgac gggcgttcgt gcatcgacca
      781 ggcgccgtcg ggcaaggtgc tgcaacccga cgacgagatc gtgagcgtcg acgggcacga
      841 gatcgagacg atcgacgact tgttgccggt gctggccggt caccagggag gtgacatggt
      901 cgaggtgcgc tacatccgcg ctggcgagga cacgccgcgc accgatcaga tcgagctgct
      961 cgagaccggt gccggcaccg aggacgaccc caagcgcgtc atcatcggct tttacccgtt
     1021 cgacaccacc acgatcagca gctcaccgtt cccgatcgag ttcgacttgg cgggcgtcgg
     1081 tgggccgtcg gccgggctgg ccttcacgtt gacgctgatc gacgtgttga cccccggcag
     1141 cttgaccggg gggcacgagg tcgcggtcac cggcgagatc aacgtnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnntacagcc ctgaagaaag aggtcgcccg gctccgggcg gagcgtgaca
     1321 tcctcaacaa cttgagggtg gcgacgctgg acgaagcctt ggccgtgttg gcccgcatcg
     1381 gtggcaatgc cgactcgatc ggcacccccg gcgccgactg ggccggcaac tgattgccac
     1441 cactcccgcg ggccccgcat ctacgctgct tcgaatggcc atctccttca cgcgcccaga
     1501 cccggcatcg gccgtgtcgg tgtcgtcggc gcagtttccg acgtcgcggc ggggtttcga
     1561 ccagaac
//
LOCUS       DAORNF010000801         1566 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_55807_length_1566_cov_1.467902, whole genome shotgun sequence.
ACCESSION   DAORNF010000801 DAORNF010000000
VERSION     DAORNF010000801.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55807_length_1566_cov_1.467902"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(72..1442)
                     /locus_tag="PLV68_21570"
     CDS             complement(72..1442)
                     /locus_tag="PLV68_21570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03900.1"
                     /translation="MIIVDKALAARERAGTPVRVGLIGAGFMGRGVASQIIRHIPGMS
                     LVAVANRTLATAARAYTEAGVSDFVHVHTQDALNHCIAKGIPAVTDDATLLCRAEGID
                     VLIEATGHVEFGAQVTTLAIEERKHIVSMNAELDGTVGPILKVMADRAGIVFSGCDGD
                     QPGVQLNLYRFVKSLGLEPVLCGNIKGLQDRYRNPTTQAGFAKRWGQSAEMVTSFADG
                     TKISFEQAIVANATGMRVARRGMLGYEFNGHVDEMTGRYDVDELRALGGIDLPTVQRY
                     LNFHFTVSLDLFGAKPGPGVFVFATTDEPLQKHHLNLYKLGEGPLYSFYTPYHLVIFE
                     VALSAARAVLFGDAVIAAQGAPTVDVITRAKVDLKAGDVIDGLGGYKTYGECENVDVV
                     RAENLLPIGLALGCRVKRDLARDEVLTHADVEIPAGRLSDRLRAEQEAHFSASPTGAA
                     SHASGN"
BASE COUNT          270 a          518 c          521 g          257 t
ORIGIN      
        1 ggtccaagcg gttggcatgg acgatgatgc ggtgctgcgg gacatcgata caccggccga
       61 tctcgccgat ctcaattgcc gctggcgtgc gaagccgcac ccgtgggtga cgcgctgaag
      121 tgcgcctcct gctcggcgcg caaccggtcc gacagccggc cggcgggaat ctcgacatcg
      181 gcgtgggtca gcacttcgtc gcgggcaagg tcgcgcttga cccggcagcc gagggcaaga
      241 ccgatcggca gcaggttctc cgcccgcacc acgtccacgt tctcgcactc gccgtaggtc
      301 ttgtagccac cgaggccgtc gatgacgtcg ccggccttga gatcgacctt ggcgcgggta
      361 atgacgtcga ccgtcggcgc cccttgcgcg gcgatcaccg catcaccgaa cagcaccgcg
      421 cgcgccgccg acaacgccac ctcgaaaatg acgaggtgat agggcgtata gaagctgtag
      481 agcggcccct cgcccagctt gtagaggttg aggtggtgct tttgcagcgg ctcatccgtg
      541 gtggcgaaaa caaaaacgcc cgggcccggc ttggcgccga acaggtcgag cgacaccgtg
      601 aagtggaagt tgagatagcg ctgcacggtc ggcaggtcga tgccgcccag cgcgcgcagc
      661 tcatcgacat cgtagcggcc ggtcatctcg tcgacgtggc cgttgaattc gtagccgagc
      721 atgccgcgcc tggccacccg catcccggtc gcgttggcga cgatcgcctg ctcgaaggag
      781 atcttggtgc cgtcggcgaa gctcgtcacc atctcggcac tctggcccca gcgcttagcg
      841 aagcccgcct gcgtcgtcgg attgcggtag cggtcctgca ggcccttgat gttgccgcac
      901 agcaccggct ccaggcccag gctcttgacg aaccggtaga ggttgagctg gacgcccggc
      961 tgatcgccat cgcagccgga gaagacgatc cccgcgcgat cggccatcac cttgaggatc
     1021 gggccgaccg taccgtcgag ctcggcgttc atcgagacga tgtgcttgcg ctcctcgatc
     1081 gcgagcgtcg tcacctgcgc gccgaactcg acgtgcccgg tcgcctcgat caggacatcg
     1141 ataccctcgg cccggcacag gagcgtggcg tcgtcggtga ccgcggggat gcccttggcg
     1201 atgcaatggt tgagagcgtc ttgcgtgtgc acgtgaacga aatcgctgac accggcctcc
     1261 gtataggccc gcgctgccgt cgccagcgtc cggttggcaa ccgcgaccag ggacatgcca
     1321 gggatgtggc gaatgatctg gctggcaacg ccgcggccca tgaagccggc gccgatcagg
     1381 ccgacacgaa cgggggtgcc agccctctcc cgcgctgcga gcgccttgtc gacgatgatc
     1441 atgatcgcct cagaagagcc cgttgacgcc gttctcgagc aaaggccagg agcggtcctt
     1501 ctccgaagtc tcgacaacgg ggtgcggcca gcgaatgccg agtgcgggat catcgaagcg
     1561 caagcc
//
LOCUS       DAORNF010000802         1559 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_56097_length_1559_cov_1.293218, whole genome shotgun sequence.
ACCESSION   DAORNF010000802 DAORNF010000000
VERSION     DAORNF010000802.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56097_length_1559_cov_1.293218"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1559)
                     /locus_tag="PLV68_21575"
     CDS             complement(<1..>1559)
                     /locus_tag="PLV68_21575"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HRE03901.1"
                     /translation="YAHYVGTLRAEPSVDYSFTAFSAVAGPFEYLIWITAPEQSFIAR
                     ESTLDALLGGFQANAFVFGLDRQETIVRLGYEPRDPEDLDPALGSGSADGYKALLFAG
                     LVRLTPELAIAPDLAEAWDVSDDGTVYTFVLRSDARFADGRPITADDVRASWERVADP
                     ATQSSGAATYLGDIEGVQAKLDGEADTIAGVEVVDGHTLRVTLDGPKAYFLAKLTYPV
                     TFVVDARDPAKGDRWMFEANASGPYQLDRILADEAVVFERNPNYHAVAPTRYLAYVVN
                     PGGSGISLFEAGEIDVAGLASEDALRIRGDATDPLHDLLRTVPSMCTTYLQIDPARAP
                     FDDPDVRRAFALAVDRDGLVERLTSNTDLAATTILPPAMPGFSADHAAYTYDPEGARA
                     ALEASTYAGKTLPTITLTVGGYGGDPGDYVNALISTWRDALGVTVRVEQLDPVEFPRL
                     TREGERTGQILAGGWCADYPDPENFLDALFFPGKEYNYAALDNATLNGLLESARVEAD
                     PATRLRLYAEA"
BASE COUNT          261 a          496 c          541 g          261 t
ORIGIN      
        1 ctgcttcggc atataaccga agccgcgttg cgggatcggc ctcgacgcgg gccgattcga
       61 gaaggccgtt cagcgtggca ttgtcgagcg ccgcgtagtt gtattccttc cccggaaaga
      121 agagcgcatc caggaagttc tcagggtcgg ggtagtcggc gcaccaaccg ccggccagga
      181 tctgcccggt acgttcgccc tcgcgagtta gtcgcggaaa ctcgaccggg tcaagctgct
      241 cgacacgtac cgtgacgccg agcgcatcgc gccaggtgct gatcagcgcg ttgacgtagt
      301 cgcccgggtc gccgccgtaa ccgccgacag tgagcgtgat cgtcgggagc gtcttgccgg
      361 cgtaggtgga ggcttcgagc gctgcccggg ctccctcggg atcataggtg taagccgcat
      421 ggtcggccga gaagcccggc atcgccggcg gcaggatcgt ggtcgccgct agatcggtgt
      481 ttgaggtcag gcgctcgacg agtccgtcgc ggtccacggc aagcgcaaac gctcgccgaa
      541 catcggggtc gtcgaacggc gcgcgtgccg ggtcgatctg gaggtaggtg gtgcacatcg
      601 acggcacggt ccgcaggaga tcgtggagcg ggtcggtcgc atcaccgcga atgcggagtg
      661 cgtcttcgct ggccagcccg gcgacgtcga tctcgccggc ctcgaagagg ctgatgcctg
      721 agccacccgg gttgacgaca taggccagat accgcgtcgg cgcgaccgcg tgatagttgg
      781 gattgcgctc gaagacgacg gcctcgtcag ccaggatgcg atcgagctga tagggtccgc
      841 tggcgttcgc ctcgaacatc cagcgatcac ccttggcagg gtcgcgggca tcgacgacga
      901 aggtcactgg gtaagtcaac ttggccagga agtacgcctt cggaccgtcg agcgtcactc
      961 gcagggtgtg tccgtcgacc acctcgacgc ccgcgatcgt atcggcctcg ccatcgagct
     1021 tcgcctggac gccctcgatg tcgccgaggt aggtcgcggc cccgctcgat tgggtcgcgg
     1081 ggtctgcgac gcgctcccag ctggcacgga cgtcgtcggc cgtgatcggt cggccgtcgg
     1141 caaagcgcgc gtcgctgcgc aacacgaacg tgtacaccgt gccgtcgtcg ctgacgtccc
     1201 acgcctcggc cagatccggc gcgatcgcaa gttctggcgt cagccggacg agcccggcga
     1261 acaacagcgc cttgtagcca tcggccgagc cgctgcccag ggccgggtcg aggtcctcgg
     1321 ggtctctggg ctcgtatccg agacgcacga tcgtctcttg acggtccagc ccgaacacaa
     1381 aggcgttggc ctggaacccg ccgagcaggg cgtcgagcgt cgactcgcgc gcgatgaagc
     1441 tttgctcggg agccgtgatc catatcaggt actcaaacgg cccggcgact gccgagaacg
     1501 ccgtgaacga atagtcgacg gatggttcgg ctcgaagcgt gccgacatag tgagcgtac
//
LOCUS       DAORNF010000803         1558 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_56116_length_1558_cov_2.075183, whole genome shotgun sequence.
ACCESSION   DAORNF010000803 DAORNF010000000
VERSION     DAORNF010000803.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56116_length_1558_cov_2.075183"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>277
                     /locus_tag="PLV68_21580"
     CDS             <1..>277
                     /locus_tag="PLV68_21580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03902.1"
                     /translation="VPLGPGIDGAGYELEPPFSPPGSGERTEGRFQSDLVSFGSNGIE
                     IRFAEGAWMSPGPVSLWSRLTVPVIAGEEPTGPQRAATTADFSNGVSA"
     assembly_gap    278..346
                     /estimated_length=69
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    670..689
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<690..1278)
                     /locus_tag="PLV68_21585"
     CDS             complement(<690..1278)
                     /locus_tag="PLV68_21585"
                     /inference="COORDINATES: protein motif:HMM:TIGR03856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HRE03903.1"
                     /translation="MSTFRIGVQIQPQHADYRQIRAGVAKAEEAGVDIVFNWDHFFPL
                     YGEPDGLHFECWTMLGAWAEATERVEIGALVSCNSYRNPELLADMARTVDHISNGRLI
                     LGIGAGWFERDYDEYGYEFGTAGSRLADLAQALPRIRRRWGQLNPAPTRDIPILIGGG
                     GERKTLRMVAEHADIWHSFSNAETLRSKSEVLERWC"
BASE COUNT          215 a          520 c          506 g          228 t
ORIGIN      
        1 gtcccgctcg gcccaggcat cgacggcgcc ggatacgagt tggagccacc gttcagcccg
       61 cccggcagcg gcgagcgcac cgaaggacgg ttccagtccg acctggtgtc gttcggttcc
      121 aacgggatcg agatccgctt cgccgagggt gcctggatga gccccgggcc ggtctcgctg
      181 tggtcgcgcc tgaccgtgcc cgtgatcgcc ggtgaggagc cgaccggccc gcagcgggcg
      241 gcaaccacgg ccgacttcag caacggggta tcggccgnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnggaa ggcgagtgga
      361 tcgggatgca ggcccgctcg ttctacggcg agcgtggcgc aggctttgcc gacggcgcgc
      421 tgttcgatca gcgcggccgc tgcgggcgca gcgcgcagag cctgatcatc gtcgagcgct
      481 gatccgcacc acagcggacg actactgggc gtcgcgccag gcgacccatt ccttgaccag
      541 cccgaggtcg tagtcgggtc cgttgatgcc gatggtgaac agcgtcgcac ccgcggcgac
      601 catctcatcg gcgaccaggt cgggcgactt gcgtgcttcg accgaacgct cgatggcggc
      661 cgggttgcgn nnnnnnnnnn nnnnnnnnnc gcaccaccgt tcgagcactt cggacttcga
      721 ccgcagcgtc tccgcgttgc tgaacgagtg ccagatgtcg gcgtgctcgg ccaccatccg
      781 cagcgtcttg cgctcgccgc cacccccgat caggatcggg atgtcgcgcg tgggcgccgg
      841 gttgagctgg ccccaccggc ggcggatgcg cggcagggcc tgagccaggt cggccaggcg
      901 gctgcccgcg gtgccgaact cgtatccgta ttcgtcgtag tcgcgctcga accaaccagc
      961 accgatgccg aggatgagac ggccgttgct gatgtggtcg accgtgcgcg ccatgtcggc
     1021 cagcagttcg gggttgcgat acgagttgca cgacaccagg gcgccgatct cgacccgctc
     1081 ggtggcctcg gcccacgcgc cgagcatggt ccagcactcg aagtgcagcc cgtcgggctc
     1141 cccgtagagc gggaagaagt ggtcccagtt gaacacgatg tcgaccccag cctcttccgc
     1201 cttggccacg ccggccctga tctggcggta gtccgcgtgc tgcggctgaa tctgtacccc
     1261 gatgcgaaac gtgctcatgg ccgcacccta ccgaagccac cgggcgtgag ggtcggttga
     1321 gccgacctcg ctttggtccc tgattcgtcc cgcggccgtc ccttgccgtc agaggccccg
     1381 cggcgtggca actaccgtga tcggccatga gcgactccga ccccaaccgg ccgctgaaca
     1441 cctgggcgac cgcgggtggg tccgtgccgt acgtgccggg gccaccccgc cccaaccgtc
     1501 ccggtgcacc cggtcagacc ggcaccgcgc agggcagcga catcgaccgc atcctggg
//
LOCUS       DAORNF010000804         1556 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_56210_length_1556_cov_1.570953, whole genome shotgun sequence.
ACCESSION   DAORNF010000804 DAORNF010000000
VERSION     DAORNF010000804.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56210_length_1556_cov_1.570953"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>217
                     /locus_tag="PLV68_21590"
     CDS             <1..>217
                     /locus_tag="PLV68_21590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03904.1"
                     /translation="KALYSALTTAAVGIVAYFALQPYFDSDSYGIRNTLLLGVVAVVV
                     GVVVGLAYGGFDKGLSARTAGITALLIS"
     assembly_gap    218..260
                     /estimated_length=43
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <261..755
                     /locus_tag="PLV68_21595"
     CDS             <261..755
                     /locus_tag="PLV68_21595"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRE03905.1"
                     /translation="RNRPIKTIGDRESRLKGSFWVISNDTFSHLILPTLALMLISLAS
                     YTRYSRSSMLEVLNQDYIRTARAKGLTERTVVIRHGFRNAMIPLATIVAFDIGGVLGG
                     AVITETVFEWSAMGRMFIDALRDLDPNPMMAFFVVISLFAVVANLVADLLYAVLDPRI
                     RLTK"
     gene            766..>1556
                     /locus_tag="PLV68_21600"
     CDS             766..>1556
                     /locus_tag="PLV68_21600"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRE03906.1"
                     /translation="MTLNAGPHDSDLLGSGHTPGAPVVDTDEMSLEQLEVAGLSQSQI
                     VRRRFFRHKGAMSSLILLVLILLLSITSIGAGPVPGWWKYDYNTVVPNTPDEHPTMSF
                     RPEFLGGSGIQIGDHPFGLDNSKGRDIFALTMRGVQMSMVVIVALALLSVTIGVVIGA
                     LSGYFGGWLDSILMRATDVILIIPLLLIAAVAGFAFKASGLWSVAIVLGLLSWTGLAR
                     LVRAEFLAFREREFVDAAKVAGATSRRIIFKHILPNTVGTIIVSTT"
BASE COUNT          231 a          508 c          470 g          304 t
ORIGIN      
        1 aaaaggcgct gtacagcgcg ctcaccaccg ctgcagtcgg catcgtggcc tactttgcac
       61 tccagccgta cttcgactcc gactcgtacg gcatccggaa cacgctgttg ttgggggtcg
      121 tcgccgtcgt ggtcggcgtc gtcgtcggcc tcgcctacgg cgggttcgac aagggattgt
      181 cggctcgtac ggcgggtatc accgccctgt tgatctcnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn cgcaaccggc cgatcaagac catcggcgat cgcgagtctc
      301 gcctcaaggg ctcgttctgg gtgatcagca acgacacgtt cagccacctg atcctgccca
      361 cgttggcgtt gatgctgatc tcgctggcct cgtacacccg ctattcgcgc tcgagcatgt
      421 tggaagtgct caaccaggac tacatccgca ccgcccgcgc caagggcctc accgagcgca
      481 cggtcgtgat ccgccacggg ttccgcaacg cgatgatccc gttggccacc attgtcgcct
      541 tcgacatcgg cggcgtactc ggcggggccg tgatcaccga gacggtgttc gaatggtcgg
      601 cgatggggcg gatgttcatc gacgcgctgc gcgacctcga tcccaacccg atgatggcct
      661 tcttcgtcgt catctccctg ttcgccgtcg tcgccaacct ggtggccgac ctgttgtacg
      721 ccgtgctcga tccgcgcatc cggctgacga agtgaggagc gagccatgac gctgaacgcc
      781 ggcccccacg actccgacct cctcggatct ggccacacgc ccggggcacc tgtggtcgac
      841 accgacgaga tgagcctcga gcaactcgaa gtggccgggc tcagccagag ccagatcgtc
      901 cgccgccggt tcttccgcca caagggcgcg atgagctcgc tgatcctgct ggtgctgatc
      961 ctgctgttgt cgatcaccag catcggtgcg ggcccggtgc ccggctggtg gaagtacgac
     1021 tacaacaccg tcgtgccgaa cacgcccgac gagcacccga ccatgtcgtt ccggcccgag
     1081 ttcctcggcg gcagtggcat ccagatcggt gaccatccgt tcggcttgga caactcgaaa
     1141 ggccgtgaca tcttcgccct cacgatgcgc ggcgtgcaga tgtccatggt cgtcatcgtc
     1201 gccttggccc tgctctcggt gacgatcggt gtggtcatcg gcgcgctgtc gggctacttc
     1261 ggtggctggc tcgattccat cctgatgcga gccaccgacg tcatcctgat catcccgttg
     1321 ttgttgatcg cggccgtcgc cgggttcgcg ttcaaagcga gcgggttgtg gagcgtggcg
     1381 atcgtgctcg gcttgttgtc gtggaccgga ttggcgcgcc tcgtacgcgc cgagttcctg
     1441 gcctttcgcg agcgcgagtt cgtcgacgcg gccaaggtgg ccggggctac gtcgcggcgg
     1501 atcatcttca agcacatcct gcccaacacg gtgggcacga tcatcgtgtc gaccac
//
LOCUS       DAORNF010000805         1549 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_56493_length_1549_cov_1.642570, whole genome shotgun sequence.
ACCESSION   DAORNF010000805 DAORNF010000000
VERSION     DAORNF010000805.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56493_length_1549_cov_1.642570"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..753
                     /locus_tag="PLV68_21605"
     CDS             <1..753
                     /locus_tag="PLV68_21605"
                     /inference="COORDINATES: protein motif:HMM:NF014072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LarC family nickel insertion protein"
                     /protein_id="HRE03907.1"
                     /translation="PIAVSHGTVRAAHGQIPVPAPAVAALLAKRRAPAIGLDTHLEIA
                     TPTGVALMTALADEFGPMPAMTVTNTGFGAGTKDVDGRPNVVQVVIGEATVQSAAASA
                     GQPVRLLEVNVDDVTGEVVAHTIAAVLGAGAHDAWATPIVMKKGRPAHTIHALCDPAR
                     TADVAATLVRESGSLGVRGTTIERWPAQREQITVEIDGHPIRLKLSADRVKVEHDDAA
                     TAAAGLGWPLRVVLNAAESLGWAARASGDTPG"
     assembly_gap    1044..1104
                     /estimated_length=61
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1105..>1549
                     /locus_tag="PLV68_21610"
     CDS             <1105..>1549
                     /locus_tag="PLV68_21610"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRE03908.1"
                     /translation="MTRLGIGDDTTVIAYDDTGGMTAARLVVMLRMLGLDAALLDGGL
                     AAWTGELTTGAGAAREPRHFTARSWPVERLASVDEIALAAARGSASPGMVLTDARPSG
                     RYNGEITAVDPRPGHIPGAVNAPWSAVVDPATTKLLPIPELRAHFA"
BASE COUNT          220 a          555 c          511 g          202 t
ORIGIN      
        1 ccgatcgcgg tcagccacgg caccgtgcgc gccgcccacg gccagatccc cgtgcccgcg
       61 cctgcggtcg cggcgctgct cgccaagcga cgggcacccg cgatcgggct ggacacacac
      121 ttggagatcg ccacaccgac cggggttgcg ttgatgaccg cgctcgcgga cgagttcgga
      181 ccgatgcccg cgatgaccgt gaccaacacc gggttcggcg ccggaaccaa ggatgtcgac
      241 gggcgcccca acgtcgtgca ggtcgtgatc ggcgaagcga cggtccagag cgccgcggcc
      301 agcgctggcc agccggtgcg cctgttggag gtcaacgtcg acgacgtgac cggcgaggtc
      361 gtggctcaca cgatcgcggc ggtgctcggt gctggtgctc acgacgcgtg ggcgacgccg
      421 atcgtgatga agaagggtcg gcccgcgcac accatccacg cgctgtgcga cccagcgcgc
      481 acggcggacg tcgcggcgac gctcgtgcgc gagtccggct cactcggggt gcgcggcacg
      541 acgatcgaac ggtggccagc acagcgcgag cagatcaccg tggagatcga cggccacccc
      601 attcgcctca agctcagcgc cgatcgggtc aaggtcgaac acgacgacgc cgccaccgcc
      661 gcggctggcc tcggttggcc attgcgagtc gtgctcaacg ccgccgaatc cctcggctgg
      721 gccgcccggg catccggcga cacacccggc tgactacctc agctaggccg cccgggcatc
      781 cggcgacaca ctcggctgac tacctcggct ggcccgagcg cgggttgacg gccgcgtggg
      841 gactcacggt tcggccaggt gggtacggtg gcgttcatgc cgattccgcc tgtggtcaac
      901 gccgaacaat tggtcgacta ccccgacgcc gtgctgtgcg acgtgcgctg gtatctcgac
      961 gggcgcagcg gtcgcgccgc ctacgaacag ggccacctgc cgggggcgat ccacgtcgac
     1021 atcgacgccg acctggccgc ccannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnncatgac ccggctcggc atcggcgacg acaccaccgt
     1141 gatcgcctac gacgacaccg gcggcatgac cgcggcgcgg ctcgtggtga tgctgcgcat
     1201 gctcggtctg gacgcggcct tgctcgacgg cggactcgcc gcctggaccg gcgaactgac
     1261 caccggcgcc ggcgcggccc gcgagccccg tcacttcacg gcgcgctcgt ggcccgtcga
     1321 gcggctggcc tctgtcgacg agatcgcgct cgccgccgct cgtggctccg cgagtcccgg
     1381 gatggtgttg acggacgccc ggcccagcgg gcgctacaac ggtgagatca ccgcggtcga
     1441 cccgcgtccc ggccatatcc ctggcgccgt caacgcgcca tggtctgctg tggtcgaccc
     1501 ggccaccacc aaactgctgc ccatccccga actgcgcgcc cacttcgcc
//
LOCUS       DAORNF010000806         1548 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_56541_length_1548_cov_1.725385, whole genome shotgun sequence.
ACCESSION   DAORNF010000806 DAORNF010000000
VERSION     DAORNF010000806.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1548
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56541_length_1548_cov_1.725385"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1464)
                     /locus_tag="PLV68_21615"
     CDS             complement(<1..1464)
                     /locus_tag="PLV68_21615"
                     /inference="COORDINATES: protein motif:HMM:NF033551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HRE03909.1"
                     /translation="MQGSSDPNREFLDAQALVGHLVPVGTVYAFLAGHRQRLFPDELF
                     ADLFGSGRGRPSVPAEVIATVLVLQSLEGLSDREALQAVRTDLRWKVAAGLRLDDEGF
                     HPTVLTLWRNKLRQSQRPERIFDAVRDVVTATGVLNGKTRRALDSTLLDDAVATQDTV
                     TQLVSVIRRTRRQVPVAAGVEVHAHEYETAGKPSCAWDDPTARAELVTGLVNDALAIL
                     AALDGVELDDDQQRLVGLLALVAGQDVEEGEQPGTWRIAHKVAYERVISTVDPETRHM
                     HKSRSEYRDGFKAHLAIEPETGLITGVDLTAANAPDGTHGVALLDGEPAGLDVLADSA
                     YGTGPARAALETAGHRAIIKPWPLARNPRLGDDQFRRDEFIIDYINRTVTCPNGITVT
                     IAAKGSANFGPRCTGCPLPSRCTANTDGKVFNVGNHDQLLAAARQQWRNSDDLDDYRQ
                     HRPMVERSIAWLVANGHRRVRYRGIDRNRPALTTPAPT"
BASE COUNT          246 a          506 c          518 g          278 t
ORIGIN      
        1 ggtgggggct ggggtggtga gtgctgggcg gttgcggtcg atgccgcggt agcggactcg
       61 gcggtggccg ttggccacga gccaggcgat ggatcgttcg accatcggtc ggtgttgccg
      121 gtagtcgtcg aggtcgtcgc tgttgcgcca ttgctgtcgt gctgccgcga ggagttggtc
      181 gtggttgccg acgttgaaga ccttgccgtc ggtgttggcg gtgcagcggc tggggagggg
      241 gcagccggtg catcgtggac cgaagttcgc ggagcccttc gcggcgatcg tcacggtgat
      301 cccgttcgga caggtgacgg tgcggttgat gtagtcgatg atgaactcgt cgcgtcggaa
      361 ctgatcgtcg ccgagtcgcg ggttgcgggc gagtggccag ggtttgatga tcgcccggtg
      421 tccggcggtc tcgagcgctg cccgggccgg gccggtgcca tacgcgctgt cggcgagcac
      481 gtcgagcccg gcgggttcac catcgagcag agcgacacca tgcgtgccgt cgggggcgtt
      541 cgctgcggtg agatcgacac cggtgatcag cccggtctcg ggctcgatcg cgaggtgcgc
      601 tttgaacccg tcgcgatact ccgatcggga cttgtgcatg tgccgtgtct ccgggtcgac
      661 cgtggagatc acccgctcat aggcgacctt gtgagcgatc cgccatgttc cgggctgctc
      721 gccctcttcg acgtcttgac cggcgaccag cgcgagcaga ccgacgagtc gctgttggtc
      781 gtcgtcgagt tcgacaccat cgagcgccgc caagatcgcg agggcgtcgt tgacgagacc
      841 ggtgacgagc tcggcacgcg ctgtcgggtc gtcccacgcg cacgacggtt tccccgccgt
      901 ctcatactcg tgcgcgtgaa cctccacacc ggctgcgaca ggcacctggc ggcgggtgcg
      961 acggatcacc gacaccaact gtgtgaccgt gtcctgggtc gcgaccgcgt catccaacaa
     1021 cgtcgagtcc aacgcccgac gggtcttgcc gttcaacacc ccggtcgctg tcacgacatc
     1081 gcggaccgcg tcgaagatcc gctccggacg ctgcgactgg cggagcttgt tgcgccacaa
     1141 cgtgagcacc gtcggatgga agccctcgtc atccaacctg agaccggccg cgactttcca
     1201 acgcagatcg gtgcgcaccg cctgcagcgc ttcacgatcc gacaggccct cgagcgactg
     1261 caacaccaac acggtcgcga tcacctcggc cggcaccgac ggccggcccc tgccggaacc
     1321 gaacagatca gcgaacaact cgtccgggaa cagtcgctgg cgatgcccag ccaagaacgc
     1381 atacaccgtc cccaccggca ccagatgacc aaccaacgcc tgcgcgtcga ggaattcccg
     1441 attcggatcc gatgagccct gcacgcccac cattcttttc gatcccgccg cccaacacga
     1501 gcacctcacc aagatttgct cagcagtctc ctagtgtcgt gagtcgtt
//
LOCUS       DAORNF010000807         1548 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_56548_length_1548_cov_1.438714, whole genome shotgun sequence.
ACCESSION   DAORNF010000807 DAORNF010000000
VERSION     DAORNF010000807.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1548
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56548_length_1548_cov_1.438714"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>223
                     /locus_tag="PLV68_21620"
     CDS             <1..>223
                     /locus_tag="PLV68_21620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03910.1"
                     /translation="RLLVVGDKVVAAARRDPPLVVGDGVHTIRQLVERVNLDPRRGEG
                     HATSLTRIRLDEVALARLTSAGLTAESVAA"
     assembly_gap    224..398
                     /estimated_length=175
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <399..>1200
                     /locus_tag="PLV68_21625"
     CDS             <399..>1200
                     /locus_tag="PLV68_21625"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HRE03911.1"
                     /translation="VLRPLEAQGGGIVEINAAPGLRMHLQPSFGKGRPVGEAIIASMF
                     ADGDDARIPLVAVAGTNGKTTTVRLIANILGEQGLRVGMTTTDGVYVEGRRIDTGDCS
                     GPKSARNVLLHPGVDAAVLETARGGVLREGLGFDRCDVAVVTNIGLGDHLGLSYISTV
                     EDLSVVKRVIVQNVTPVSGVAVLNAADPMVARMGDSCPGTVTFFACDRNHPLMAMHRA
                     QRKRVVYRDGDALVASGGGVEHRLALADIPVTQNGAIGFQVENAMAAIA"
     assembly_gap    1201..1289
                     /estimated_length=89
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1290..>1548
                     /locus_tag="PLV68_21630"
     CDS             <1290..>1548
                     /locus_tag="PLV68_21630"
                     /inference="COORDINATES: protein motif:HMM:NF014874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HRE03912.1"
                     /translation="NVFNYRGATLVADYGHNPDAIQALVNAVDNLPSGPGSKRSVVIS
                     GAGDRRDEDIRRQTEILGSAFDRVVLYQDQCQRGRADGEVLA"
BASE COUNT          210 a          427 c          423 g          224 t
ORIGIN      
        1 accgcttgct ggtggtcggc gacaaggtcg tcgccgctgc tcgccgcgac ccaccgctgg
       61 ttgtcggcga tggcgtccac accatccgcc agttggtcga gcgtgttaac ctcgacccgc
      121 ggcgtggtga aggccacgcc acctcactca cccggattcg tctcgatgag gtggcgctgg
      181 cccgcctgac gagcgccgga ttgacggcgg agtccgtggc tgcnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngt cctgcgtcca ctcgaagccc
      421 agggcggcgg catcgtcgaa atcaacgctg cgccagggct gcgcatgcac ctgcaaccgt
      481 ccttcggcaa ggggcgcccg gtcggtgaag ccatcatcgc cagcatgttt gccgatggcg
      541 acgacgcgcg cattccgctg gttgctgtgg cgggaaccaa cggcaagacg acaaccgttc
      601 gcttgatcgc caacattctg ggcgagcagg gcctgcgtgt cggcatgacg acgaccgacg
      661 gcgtctacgt cgagggtcgg cgcatcgaca ccggcgattg cagcggcccg aagagcgcca
      721 ggaacgtcct cttgcacccc ggcgtggacg cggcggtgct cgaaacggcg cgcggcggag
      781 tgctgcgcga aggcctcggt ttcgaccgtt gcgatgtcgc ggtagtcacc aatatcggcc
      841 ttggcgatca cttggggctg tcgtacatca gcacggtcga agacctttcc gtcgtgaaac
      901 gcgtcatcgt ccagaacgtc acccccgtgt ccggcgtcgc tgtcctcaat gccgccgatc
      961 cgatggtggc gcggatgggc gattcctgcc cgggaaccgt caccttcttc gcctgcgacc
     1021 gcaatcatcc gctgatggcc atgcaccgag cgcagcgcaa gcgcgtcgtc taccgggacg
     1081 gggatgcgct cgtcgcctcc ggtggcggtg tcgaacatcg gctcgcactg gccgacattc
     1141 cggtcaccca gaatggcgcc atcggcttcc aggtggaaaa tgccatggcg gcgatcgctg
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnna acgtgttcaa ttatcgcggc gccacgttgg
     1321 tcgccgacta tggccacaac ccggacgcca ttcaggccct ggtcaacgcc gtcgacaacc
     1381 tgccgtcagg ccccggcagc aagcgctcgg tggtcatcag cggtgcgggt gatcggcgcg
     1441 acgaagacat tcgccgacag accgagatcc tggggagcgc cttcgatcgg gtcgtgcttt
     1501 atcaggatca gtgccaacgc ggtcgcgctg acggcgaagt cctggcct
//
LOCUS       DAORNF010000808         1547 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_56577_length_1547_cov_1.804960, whole genome shotgun sequence.
ACCESSION   DAORNF010000808 DAORNF010000000
VERSION     DAORNF010000808.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56577_length_1547_cov_1.804960"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    248..257
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<258..1232)
                     /locus_tag="PLV68_21635"
     CDS             complement(<258..1232)
                     /locus_tag="PLV68_21635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03913.1"
                     /translation="MALRGDDDDLVVGDASSPTTDVTATSTTLGASTTVVEPTTTIAP
                     TTTVAAMVTDPLPTTDETTTTVAETTTTTTIAAVAGPFPAINADGAAVLVDSSGASTL
                     LFQGPDPDDPGPTEGESSYVASVAVQPGGATVYVGLCCEPVVGTIFSVTPPTPGSWES
                     PWWYGSALAISPDGLWFARVTYDQITVSDAAFNDIAAVPEDFELGQTYDLAWSADGAR
                     LYGIRLGHEGWTVRSFAFDGTTLTEQPSVLILPLAAAAPVQPVGFAGARGDLLFVAGI
                     DTMRVQAYDTVSLAPRADADIVLGGSATSAWVDSDGTVRWTDDARRLHV"
     assembly_gap    1294..1315
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          260 a          525 c          512 g          218 t
ORIGIN      
        1 ggtgatggac ctggtggtgc aggggctgtc gagcaaggag atcgccgtgc gcctgcacct
       61 gtcggtcaag acgatcgaga cccacgtctc ggccgtgctg cgcaaactgc aactgtcgaa
      121 ccgtcaccaa ctcacagcct gggccaacga acgccgcctc gtgtgacgcg tcatcagccg
      181 ggtcgagtca tccaacgctg cgcaagccga ttcttccgac tggtcagcgg atccagacga
      241 agctcccnnn nnnnnnngac gtgcaaccgg cgggcgtcgt cggtccagcg caccgtcccg
      301 tcgctgtcca cccaggcgct ggttgccgag ccaccgagga cgatgtcggc gtcggcacgc
      361 ggggcgagcg acaccgtgtc gtaggcctgc acgcgcatcg tgtcgattcc ggcgacgaag
      421 agcaggtcgc cacgagcccc ggcgaagcca accggttgaa cgggggccgc ggcggcgaga
      481 ggcaggatca gcacagacgg ctgctccgtc agcgtcgtgc cgtcgaaggc gaacgagcgc
      541 accgtccagc cctcgtgacc gagccggatc ccgtataggc gcgcgccgtc cgccgaccag
      601 gccaggtcgt aggtctgccc cagctcgaag tcctccggca ccgccgcgat gtcgttgaat
      661 gcggcgtcgc tgacggtgat ctggtcgtag gtgacacggg cgaaccagag gccgtctggc
      721 gagatggcca acgcactgcc gtaccaccac ggcgactccc acgagccagg cgtcggcggg
      781 gtgaccgaga agatcgtgcc cacgacaggc tcgcagcaca gcccgacgta gacggtcgcg
      841 cctcctggtt ggacggcaac cgacgcgaca tacgagctct caccctcggt cgggccagga
      901 tcgtccgggt cagggccttg aaacagcaac gtcgacgcgc cactgctgtc gacgagtacg
      961 gcggcgccat ccgcgttgat cgccgggaac ggcccagcca ccgccgcgat cgtcgtggtg
     1021 gtggtggtct ccgccaccgt cgtcgtcgtc tcgtcggtcg tcggcaatgg gtcggtcacc
     1081 atcgcagcga ccgtggtggt gggcgcaatc gtcgtcgtcg gctcgaccac tgttgtcgag
     1141 gcaccgagcg tggtcgacgt cgcggtcacg tcggtggtcg gcgacgacgc gtcgccgacc
     1201 acgagatcgt cgtcgtcacc ccggagagcc accaccgcga cgactgctgc tacagccacg
     1261 atgaccgcgc cggcgacgag tagcgccgtg tgannnnnnn nnnnnnnnnn nnnnngcaga
     1321 acccggctcc ggcggcggaa agctggggct gctgaaggcg tcggacatcg gccaatcctc
     1381 ccatgctgat cgcggctcac gatcacacca ggtgcgaacg cggccggtac gggtggcacg
     1441 atcggggcat gaacgacgcc gcacccagcg atcggcccac gcccgtcccg ccgctgcatc
     1501 cttacgccgg gcgttatcac gtaaacacgt ccatccccga cgagggc
//
LOCUS       DAORNF010000809         1547 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_56579_length_1547_cov_1.706434, whole genome shotgun sequence.
ACCESSION   DAORNF010000809 DAORNF010000000
VERSION     DAORNF010000809.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56579_length_1547_cov_1.706434"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="PLV68_21640"
     CDS             complement(<1..373)
                     /locus_tag="PLV68_21640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03914.1"
                     /translation="MATDTIGPACALAAVVAGAPGLAGPAAIAGIVGGISYVAVFIAV
                     GCLTRRTAVWSLALVFLVERLLGAALTGIAQLSPQWESRAIFVGLIDNPPERLVRDGI
                     ELAWAHLPGHGPTVVFLPGFKS"
     assembly_gap    390..455
                     /estimated_length=66
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<456..726)
                     /locus_tag="PLV68_21645"
     CDS             complement(<456..726)
                     /locus_tag="PLV68_21645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03915.1"
                     /translation="MTTPTSAPTSVTQPSHRARPRFVAILRYTLRSCLPLRRWAAILM
                     PCAGAVLFGALSHAVERSAEDAFANIAAEAIFGLVKVREGEITLRG"
     gene            complement(723..>1547)
                     /locus_tag="PLV68_21650"
     CDS             complement(723..>1547)
                     /locus_tag="PLV68_21650"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRE03916.1"
                     /translation="VTGVLAPNGAGKTTLMRAITGMTPVNQGSVTIHGANPRTDRRVQ
                     QRVALVPEDEAVPGGLTARQFVAYIADLHHVADRAAPERALATVGMIDAADRSVSGFS
                     KGMRQRTKIAAALVCDPAVLVLDEPLNGADPVQRVHLIGLFKQLGREGRTVIVSSHVL
                     NEVDRMADRVIVVIHGRLAAAGSQRAIRDAMDDRPRHVLVRGNDVRLLAGELVGLTSV
                     SGISFDSVHDGMVVETGQARDFAVALPALARRASVRLTEVRALDDSLESLFRELVR"
BASE COUNT          237 a          492 c          539 g          213 t
ORIGIN      
        1 cgcttttgaa tccgggcagg aagacgacgg tcgggccgtg gccgggcagg tgggcccagg
       61 cgagttcgat gccgtcgcgg accagccgtt cgggcgggtt gtcgatcagg ccgacgaaga
      121 tcgcccgtga ctcccactgg ggggacaact gggcgatgcc ggtcaacgcc gcaccgagca
      181 gtcgctcgac caggaacacc agagcgagcg accacaccgc ggtgcgccgg gtgaggcagc
      241 cgacagcgat gaacactgcg acgtacgaga tgccgccgac gattccggcg atcgcggccg
      301 ggccggcgag accgggagcg cccgcgacga cagcagcgag cgcgcacgcc ggcccgatcg
      361 tgtccgttgc caccagcgtt ccacccaacn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncgccg cgcagggtga tctcgccctc
      481 gcgcaccttc acgagtccga agatcgcctc ggcggcgatg ttggcaaagg cgtcctccgc
      541 actccgttcg acggcatggc tgagcgcacc gaacagcacg gccccggcgc acggcatcag
      601 gattgctgcc caccgccgca gtggcaagca ggaccgcagc gtgtagcgga gaatcgccac
      661 gaagcgggga cgagcgcggt gggagggctg ggtgaccgag gtgggggctg acgttggtgt
      721 cgtcatcgga ccagttcccg gaagaggctc tccagcgagt cgtcgagggc gcggacctcg
      781 gtcagccgca ccgatgctct gcgagcgagt gccggcagcg caacggcgaa gtcgcgggcc
      841 tggccggtct cgaccaccat cccgtcgtga acggagtcga agctgatccc ggacactgac
      901 gtcaggccga ccagttcgcc agctagcagc cgcacatcgt tgccgcggac cagcacatgg
      961 cggggtcgat cgtccatcgc gtcgcggatg gcgcgctggc tgccggccgc ggcgagacgg
     1021 ccgtggatga cgacgatcac ccggtcggcc atccgatcga cctcgttgag gacgtgcgag
     1081 ctgacgatca ccgtgcgccc ctcacgtccg agttgcttga acagcccgat cagatgcacg
     1141 cgctgcacag ggtcggcgcc gttgagtggt tcgtcgagca cgaggacggc cgggtcgcag
     1201 accaaggcgg cggcgatctt ggtgcgctgg cgcattccct tgctgaagcc gctgacggag
     1261 cggtcggcag cgtcgatcat ccccactgtt gccagggctc gctctggcgc ggcccggtcg
     1321 gcaacgtggt gcaggtcggc gatgtaggcc acgaactgtc gcgcggtgag tccgccaggc
     1381 acggcctcgt cttcgggcac gagcgcgacc cgctgctgga cgcggcgatc ggtacgcggg
     1441 ttggctccgt ggatggtgac gctgccttgg ttgaccggcg tcatcccggt gatcgcgcgc
     1501 atcagcgtcg tctttccggc cccgttgggg gcgagcacgc cggtgac
//
LOCUS       DAORNF010000810         1546 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_56625_length_1546_cov_1.717639, whole genome shotgun sequence.
ACCESSION   DAORNF010000810 DAORNF010000000
VERSION     DAORNF010000810.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56625_length_1546_cov_1.717639"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..723)
                     /locus_tag="PLV68_21655"
     CDS             complement(<1..723)
                     /locus_tag="PLV68_21655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRE03917.1"
                     /translation="MVRALDGQYPGRGVLGTAGMQRFRNFINGSFVDAADGATTAVID
                     PSTGQQYGEAALSRDADVDAAMAAAAAAFDTWQDSTPAERSLALFRIADAVEARAEEL
                     IALEVANTGKPIAMTRSEEIPPMVDQIRFFATAARHLEGKSAGEYMRGMTSYVRREPV
                     GVCAQVAPWNYPMMMAVWKFAPAIAAGNTIVLKPSDTTPATTGLLAEIAAEFLPPGVF
                     NVINGDRDTGRALVSHKTPQMVA"
     gene            792..>1546
                     /locus_tag="PLV68_21660"
     CDS             792..>1546
                     /locus_tag="PLV68_21660"
                     /inference="COORDINATES: protein motif:HMM:NF017072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HRE03918.1"
                     /translation="MTANGSSSAPAITTVGVPKETKTAEHRVAMTPDGVRELERHGID
                     VFVETGAGAGASFTDADYVAVGATIVPTAADAWSQQMVAKVKEPTTAELAFLRPDLTL
                     FTYLHLAAYPEVAHALLAASTTGVAYETVQTASGALPLLAPMSEVAGRLAPQMGAHYL
                     ERHNGGRGVLMGGAPGVRPAKVVVLGAGNVGWNAAWIAAGMEAEVVLFDKNLDRLRWV
                     DQIQRGRVVTLASNRGAIERHVADADLLIGAVLV"
BASE COUNT          248 a          530 c          534 g          234 t
ORIGIN      
        1 cgcgaccatc tgcggggtct tgtgagagac cagggcccgg ccggtgtcgc ggtcgccgtt
       61 gatgacgttg aacacccccg gggggaggaa ctcggccgcg atctcggcca gcagacccgt
      121 ggtcgctggg gtggtgtcac tcggtttgag cacgatcgtg ttgcccgccg cgatcgccgg
      181 ggcgaacttc cacaccgcca tcatcatcgg gtagttccac ggcgcgactt gggcacacac
      241 gccaaccggc tcgcggcgaa cgtagctggt catgccgcgc atgtactcgc cggccgactt
      301 gccttcgagg tggcgcgccg cggtggcgaa gaacctgatc tggtcgacca tcggcgggat
      361 ctcttcgctg cgggtcatgg cgatcggctt gccggtgttg gcgacctcca acgcgatcag
      421 ctcctccgca cgagcctcga ccgcgtccgc gatgcggaac aacgccaggg aacgctcggc
      481 cggggtcgag tcctgccagg tgtcgaacgc agccgccgca gcggccatcg cggcatcgac
      541 gtcggcatcg cgcgacaacg ccgcttcgcc gtactgctgc ccggttgacg gatcgatgac
      601 cgcggtggtc gcaccgtcgg cggcatcgac gaaggagccg ttgatgaagt tcctgaagcg
      661 ctgcataccc gcagttccta gcacgccccg gccggggtac tgaccgtcga gagcgcgcac
      721 catggaacaa acgtgccgca cccgagccgt cgacggcatg gctgtagaac ggattgtgca
      781 agcatgccca catgacagcc aacggttcat ccagcgcccc cgcgatcacg acggtcgggg
      841 ttcccaagga gaccaaaacc gccgagcacc gggtcgccat gacgcccgac ggcgtgcgtg
      901 aactcgaacg ccatggcatc gacgtgttcg tcgagaccgg tgccggagcg ggcgcgagct
      961 tcaccgacgc cgactacgtc gcggtcgggg ccacgatcgt gccgaccgcg gccgacgcct
     1021 ggtcgcagca gatggtcgcc aaggtcaagg agcccaccac cgccgaactg gcctttcttc
     1081 gcccggatct gacgctgttc acgtacctgc acctggccgc gtacccggag gtcgcccacg
     1141 ccctgctcgc ggcgagcacg accggggttg cctacgagac cgtccagacc gcatccggcg
     1201 cactgccgct gttggcgccg atgagcgagg ttgccggacg gctcgcgccg cagatgggtg
     1261 ctcactacct cgaacgacac aacgggggac gcggcgtgct gatgggcggt gcgcccggcg
     1321 tgcgcccggc caaggtcgtc gtgctcggcg cgggcaatgt cggctggaac gcggcgtgga
     1381 tcgccgcggg gatggaggcc gaggtcgtgc tgttcgacaa gaacctcgac cgcctgcgct
     1441 gggtcgacca gatccaaagg ggccgggtcg tcacgctggc ctccaaccgc ggcgcgatcg
     1501 agcgtcacgt cgccgacgcc gacctgctga tcggcgcggt gctggt
//
LOCUS       DAORNF010000811         1546 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_56629_length_1546_cov_1.682093, whole genome shotgun sequence.
ACCESSION   DAORNF010000811 DAORNF010000000
VERSION     DAORNF010000811.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56629_length_1546_cov_1.682093"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="PLV68_21665"
     CDS             complement(<1..201)
                     /locus_tag="PLV68_21665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRE03919.1"
                     /translation="MSDPTDTHDTPGGDTPARRSSLSLFDLTGKVAVVTGGSRGLGRE
                     MVLAFAEHGADVVIASRKLANCE"
     assembly_gap    389..551
                     /estimated_length=163
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<552..869)
                     /locus_tag="PLV68_21670"
     CDS             complement(<552..869)
                     /locus_tag="PLV68_21670"
                     /EC_number="2.3.1.275"
                     /inference="COORDINATES: protein motif:HMM:NF014690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HRE03920.1"
                     /translation="MNAGDAVVTAVAVVVSYVIGMFPSAMLVARASGVDITASGSGNP
                     GASNVTRVLGWKRGVVVFALDAIKGALPAAAGWAVVDRHVGYICAAAATIGHMFPAVV
                     RFGG"
     gene            complement(914..>1546)
                     /gene="mftC"
                     /locus_tag="PLV68_21675"
     CDS             complement(914..>1546)
                     /gene="mftC"
                     /locus_tag="PLV68_21675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443074.1"
                     /note="MftC is a radical SAM/SPASM enzyme that catalyzes
                     the first two steps in biosynthesis of the electron
                     carrier mycofactocin from the terminal Val-Tyr dipeptide
                     of the precursor peptide MftA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0031179 - peptide modification [Evidence
                     IEA];
                     GO_process: GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin radical SAM maturase"
                     /protein_id="HRE03921.1"
                     /translation="LDEFRALADRFGAQLRVTRLRPSGRGADAWHDLHPTNAQQREIY
                     EWLLAHGDNVLTGDSFFHLNALGEEPLPGLNMCGAGRVVCLIDPIGDVYACPFVIHDQ
                     FKAGSVRDPGGFAQVWTDSELFLGLREPESAGACSSCGHYDACQGGCMAAKFFTGLPL
                     DGPDPECVNGHGEFAVLSADRDLTPKPSMDHSKPVRVQLGAKPNVLVGAR"
BASE COUNT          220 a          467 c          500 g          196 t
ORIGIN      
        1 ctcgcagttg gccaacttgc ggctggcgat cacgacgtcg gcaccatgtt cggcaaaggc
       61 caacaccatc tcccggccga gtccccggga gccgcccgtg acgaccgcga ccttgccggt
      121 cagatcgaac agggagaggc tgctgcggcg ggcaggggtg tcgccgccgg gggtgtcgtg
      181 cgtatctgtc gggtcgctca tggaccgatt cttgccgttc cgcgccgcgg gaggcacatg
      241 caagggtcag cgcggcgtgc cgaccgagtg ctcggcgccg cgcgcgaggc gcttgatgtt
      301 gtcggtgtgg cgcaccgcca ccaacgcggc caaggcgacg atcgcgacga tctcccaggc
      361 cggtgcaccg gcgatggcgg ccccgatcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      541 nnnnnnnnnn naccgccgaa gcggacgacg gcggggaaca tgtgcccgat cgtggccgcg
      601 gcggcgcaga tgtagccgac gtggcgatcg accactgccc aaccggccgc agcgggcaga
      661 gcccccttga tggcgtcgag ggcgaacacg accacgccac gcttccagcc gagcacgcgg
      721 gtgacgttcg acgcgccagg gttgcccgac ccgctggcgg tgatgtcgac gccgctggcg
      781 cgggccacca acatcgcgct ggggaacatg ccgatcacgt acgagaccac gaccgcgacc
      841 gcggtgacga cggcgtctcc agcattcatc gcccgagggt ttaacagcgc gccgggggcc
      901 ggtgcgaatt ccgtcagcgg gcgccgacga gcacgttcgg tttggcgccg agttggaccc
      961 gcaccggctt gctgtgatcc atcgacggct tcggggtgag gtcgcggtcc gcgctgagga
     1021 cggcgaactc gccgtgcccg ttgacgcact cgggatcggg gccgtcgagc ggcaacccgg
     1081 tgaagaactt ggccgccatg cacccccctt ggcaggcgtc gtagtgaccg cagctactgc
     1141 acgcaccggc cgattcgggc tcgcgcaggc cgaggaacaa ctcgctgtcg gtccacacct
     1201 gggcgaagcc gccggggtcg cgtaccgaac cggccttgaa ctgatcgtga atgacgaacg
     1261 ggcaggcgta gacatcgccg atcggatcga tcaggcacac cacccggccc gcgccacaca
     1321 tgttcagtcc cggcaacggc tcttcgccga gcgcgttgag gtggaagaac gagtcgccgg
     1381 tcaacacgtt gtcaccgtgg gcgagcagcc actcgtagat ctcgcgttgc tgggcgttgg
     1441 tcgggtgcag gtcgtgccac gcgtcggcgc cgcggccgct ggggcgcagc cgggtgacgc
     1501 gcagttgggc gccgaaccgg tcggccaggg cgcggaactc gtcgag
//
LOCUS       DAORNF010000812         1542 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_56782_length_1542_cov_1.891056, whole genome shotgun sequence.
ACCESSION   DAORNF010000812 DAORNF010000000
VERSION     DAORNF010000812.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56782_length_1542_cov_1.891056"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            120..794
                     /locus_tag="PLV68_21680"
                     /pseudo
     CDS             120..794
                     /locus_tag="PLV68_21680"
                     /inference="COORDINATES: protein motif:HMM:NF014805.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
     gene            1213..1491
                     /locus_tag="PLV68_21685"
     CDS             1213..1491
                     /locus_tag="PLV68_21685"
                     /inference="COORDINATES: protein motif:HMM:NF014225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03922.1"
                     /translation="MGKLSEGAAVTAVDLIAAGTISFVINTPQGRGGRTDGEQIRKAA
                     NVHQVASVTTVDAALAAVQGMAEQTGRPVEVRTLQEYHRRPVPVPVLA"
BASE COUNT          236 a          530 c          552 g          224 t
ORIGIN      
        1 tcagcaccga ctacgacacc agcgaccggc tgtacttcga accgctgacc aaggaggacg
       61 tgctcaacgt catcgacgcg gagacggtgg ccagtggggg agtgagcccg aaggtgatcg
      121 tgtcactcgg cggccagacg ccgttgaagc tgtcgggcga gttgccggtc gagctgattg
      181 cgggcacctc accggcttcg atcgacctgg ccgaagaccg cgagaagtgg gccgcgctgt
      241 gcaacgacct gcgcatcccg cagccgcccg gcggcaccgc gctcgacttg gaccaggccc
      301 tcgcgatcgt ggccgatgtc gggttcccgg tgttggtccg acccagctac gtgctgggcg
      361 ggcgcggcat gcagatcgtg cacgacaccg aacatctcgc ccgggcgatg gccgagttga
      421 ccggcttcgg cagcctcggg cgcgagggcg gcctgtcggc ggagcggccc gtgctgatcg
      481 accgcttcct tgaagacgcg accgagatcg acgtcgacgc gatccgcgac cacagcggcg
      541 aggtcgtcat cggcggcgtg atggaacacg tcgaagaggc cggcgtgcac agtggcgatt
      601 cggcctgcgc gatcccgccg cagacgctgc ccgcgtgggt ggtcgaggtg gccgaggcct
      661 acacccgctc gatcgctgcc gcgctcgacg tgcgcggcct gatcaacgtg cagttcgccg
      721 tggccggcac caccgtgtac gtcatcgagg ccaatccccg cgcgagccgg accgtgccgt
      781 tcgtggccaa ggccaccggg gtgccgttgg ccaaggtggc cagccgggtg atgctcgggg
      841 ccacgctggc cgagctgcgt accgaaggcc tgctggtcga gccggtgcag gggccgcacg
      901 tcagcgtcaa ggaagcggtg ctgccgttca gccggttccc ccaggtcgac cccgcgctcg
      961 gtcccgagat gcgctcgacc ggcgaggtga tgggtatcgc gtccagcttc ggcaaggcgt
     1021 tcttcaaggc cgaactcgcg gccggaacgg tgttgcccac cgtcggcacg gtgttcttgt
     1081 cgctcgccga cgccgacaag ccggcgggca tcgtggtcgc ctctgcattg cccggcctgc
     1141 ggcaccgaga gcgacgccac caccggcact gccgactacc tggcccgctt cggcatcgtc
     1201 gtcgaccagg tcgtgggcaa gctcagcgaa ggtgccgcgg tcaccgcggt cgacctgatc
     1261 gcggcgggca cgatcagctt cgtgatcaac accccgcagg gccgtggcgg acgcaccgac
     1321 ggcgagcaga tccgcaaggc cgccaacgtc catcaggtcg cgtcggtgac cacggtcgac
     1381 gcagccctcg ccgccgtgca gggcatggcc gagcagaccg gccgtccggt cgaggtgcgg
     1441 acgctgcagg aataccaccg ccgcccggtg ccggtgccgg tgctcgcgtg agccaggtct
     1501 gagcccagcg tgagcggcgt cgtgaacagt gtcgacacac gg
//
LOCUS       DAORNF010000813         1533 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_57180_length_1533_cov_1.577131, whole genome shotgun sequence.
ACCESSION   DAORNF010000813 DAORNF010000000
VERSION     DAORNF010000813.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1533
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57180_length_1533_cov_1.577131"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            24..>901
                     /locus_tag="PLV68_21690"
     CDS             24..>901
                     /locus_tag="PLV68_21690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03923.1"
                     /translation="MTSVVAVLTNEAHTVAADSTGSVTAGNFSGCGGTMKVYRGLTQV
                     TTGITWSIAVNASSLTASIDVSGQYSATAQGGWATSSRSAVLTLRATHADFVLDKDFT
                     VTKSVAGAAGVSSYGVAVIAPSQVFRTAKDGVVTPSSILLSANVQNLASLGAVAYSWS
                     TVPNIGSGTSSTFSVSSAALGANNSVEVTLQATAASTTVTDKVTLVRLAEGTNAVQGV
                     MSNEAHTFAANSSGTVTSYAGSGTEIRVYEGATELAYQAGGANGTWTVSTAQSGIQVG
                     SLTDSGSYLTVGQHSN"
     assembly_gap    902..966
                     /estimated_length=65
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <967..>1533
                     /locus_tag="PLV68_21695"
     CDS             <967..>1533
                     /locus_tag="PLV68_21695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03924.1"
                     /translation="PLQQTFSKAKAGTSGTDGVRGSVRIYTTQSDFQRYDTRVAPKAK
                     WARTGTLVGTDSSLGTAFDGDATAIVQAWLTANGLPTTLRAGDELTVTHVGGEKVATG
                     VWTGTYWQNPGVVIDGNLLVGGTVSALAIDAVYSLSVQGEAITLPVGAVTSTDTTITA
                     TSAATAQVVLNGPHGVAYQIPAAQAGSQ"
BASE COUNT          268 a          469 c          514 g          217 t
ORIGIN      
        1 ccatcgtgcg tctgcgcgac ggggtgacca gcgtcgtcgc ggtcctgacg aatgaggcgc
       61 acaccgtagc ggccgacagc acagggtcgg tgaccgcggg aaacttcagt ggctgcggcg
      121 gcacgatgaa ggtgtaccgc gggctgactc aggtcacgac cggcattacg tggtcgatcg
      181 cggtcaacgc gagcagcctg actgcgtcaa tcgacgtgtc ggggcagtac tcagctaccg
      241 cacagggggg ctgggcaacg tcaagccggt cggcggtcct caccttgcgc gcgacgcacg
      301 ccgacttcgt actcgacaag gactttacgg tcacgaaatc ggtagcgggg gcggctggcg
      361 tgtcgtccta tggggtggcg gtgatcgcgc ccagccaggt gttccgcacc gcgaaggacg
      421 gtgtggtcac accgagctcg atcctgctct ccgcgaacgt gcagaacctg gcgagcttgg
      481 gtgccgtggc ctactcatgg tccaccgtgc cgaacatcgg gagcggcaca tcgtcgacct
      541 tcagcgtgag cagcgccgcg ctcggggcga acaactccgt cgaagtgacc ctgcaggcga
      601 cggcagcgtc gacgaccgtg acagacaagg tcaccctcgt gcgcctcgca gagggaacga
      661 acgcggtgca gggcgtgatg tcgaacgaag ctcacacctt cgccgcgaac tcgtctggca
      721 ccgtgaccag ctacgccggc agcgggaccg agattcgcgt ctacgagggc gcgacggagc
      781 tggcctacca ggccggcggg gcaaacggca catggactgt gagcacggcg cagagcggca
      841 ttcaggtagg gtcgctgacc gactcggggt catacctgac ggtcggtcag cacagcaaca
      901 tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      961 nnnnnntccc gctgcagcag accttctcga aggccaaggc tggcacatcc ggcaccgacg
     1021 gcgtccgcgg atcggtgcgc atctacacga cgcagagcga cttccagcgc tacgacacac
     1081 gggtggcgcc gaaggcgaag tgggcgcgaa cgggcacgct cgtcggaacg gacagctctc
     1141 tcgggacggc gttcgatggg gacgcgaccg cgatcgtgca ggcatggctc acggcgaacg
     1201 gtttgcccac cacgttgcgt gctggcgacg agttgaccgt cacccatgtc ggcggcgaga
     1261 aggtcgccac cggcgtgtgg acgggcacgt actggcagaa cccgggcgtg gtcatcgacg
     1321 gcaatctgct ggtgggcgga accgtctctg cgctggcgat cgacgcggtc tactcgctgt
     1381 cggtccaggg cgaggccatc acgctgccgg tcggtgccgt gacatcgacg gacaccacga
     1441 tcaccgcaac cagcgccgcg acagcccaag tggtgctcaa cggacctcat ggggtcgcgt
     1501 accagatccc ggccgcacag gcgggctcgc agt
//
LOCUS       DAORNF010000814         1532 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_57233_length_1532_cov_1.422478, whole genome shotgun sequence.
ACCESSION   DAORNF010000814 DAORNF010000000
VERSION     DAORNF010000814.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57233_length_1532_cov_1.422478"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..480)
                     /locus_tag="PLV68_21700"
     CDS             complement(<1..480)
                     /locus_tag="PLV68_21700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03925.1"
                     /translation="MDADLPASNPPGGSHAIPLPPPPPAPDPSVTLADPTVADPFAWT
                     PPPLRAGQLAPSWRTTFLIGWVLVVAALGCMWQVSRQIGLATWWLGPETDQRSWLVIA
                     IPFVGPAATLAAGAWNRTWAVHLGIVVSLITGVIAFGDREYPGLMIGEGIVALSALAI
                     "
     gene            558..1301
                     /locus_tag="PLV68_21705"
     CDS             558..1301
                     /locus_tag="PLV68_21705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRE03926.1"
                     /translation="MAEPEFEKLSIFFPMWNEEEYIERALAAGIEVCDALVAEGQIGD
                     YELIVVDDASTDATPEIAGRLAAANPRVRVVHHPVNRKLGGSIKTGFAAATGDLVLYT
                     DADLPFDFQELHKALRIMRLYESDIVSAYRHDRTEEGSSRAIYTFFYNLLIRLMFGVK
                     MRDINFAFKLCKRRIFDHIQLHSEGSFIDAELVIRAKKLGYTVTQFGVDYFPRTRGQS
                     TLSSPAIILKILREMRELRGELRTIQRVV"
BASE COUNT          258 a          510 c          519 g          245 t
ORIGIN      
        1 gatcgccagc gcggacaggg cgacgatgcc ctcgccgatc atcagcccgg ggtactcgcg
       61 gtcgccgaac gcgatcacac ccgtgatcag ggacaccacg atgccgagat gcaccgccca
      121 ggtgcggttc catgcaccgg ccgccagcgt cgcggccggg ccgacgaacg ggatcgcgat
      181 caccagccac gagcgctggt cggtctcggg gccgagccac caggtggcca agccgatctg
      241 gcgcgacacc tgccacatgc agccgagggc ggcgacgacc aacacccagc cgatcaggaa
      301 cgtggtccgc cacgacgggg cgagttgccc ggcgcgcagc ggcggaggcg tccaggcgaa
      361 cgggtcggcc acggtgggat cggccagggt caccgacgga tccggggccg gcggcggtgg
      421 gggcagcggg atggcgtgac tgccgcccgg cgggttcgaa gcggggagat cggcgtccac
      481 tgacggcgag cgtacccggc gggtttcgat cgcggagcag cacgtggggc ctgccgcgac
      541 gggtaccctg tcgctccgtg gccgagcccg agttcgagaa gctgtcgatc ttctttccga
      601 tgtggaacga ggaggagtac atcgagcgcg ccctcgccgc gggcatcgag gtgtgcgatg
      661 cgttggtcgc cgagggtcag atcggcgact acgagttgat cgtggtcgac gacgcgtcca
      721 ccgatgccac ccccgagatc gccggtcggt tggccgctgc caaccctcgc gtccgggtgg
      781 ttcaccaccc ggtcaaccgc aagctggggg gcagtatcaa gaccgggttc gccgcggcga
      841 cgggtgacct ggtgctgtac accgatgccg atctgccgtt cgacttccag gagttgcaca
      901 aggcgctgcg gatcatgcgc ttgtacgaga gcgacatcgt cagcgcgtat cgccacgacc
      961 gcaccgagga aggctcgtcg cgggcgatct acacgttctt ctacaacctg ctgatccggc
     1021 tgatgttcgg tgtgaagatg cgcgatatca acttcgcgtt caagctctgc aagcggcgca
     1081 tcttcgacca catccagctc cacagtgaag gctcgttcat cgatgccgag ctcgtcatcc
     1141 gggccaagaa gctcggctac acggtcaccc agttcggcgt cgactacttc cctcgcaccc
     1201 gcggccagtc cacactgagc agccccgcga tcatcctcaa gatcttgcgc gagatgcgcg
     1261 agctgcgcgg cgagctgcgc accatccagc gcgtcgtctg atcccccacg ccggggcaga
     1321 ccggcggctg ttgcgatcac cgttgcagac cgtttccaac ccgctgaatt gcacgatttg
     1381 tgcgaaatct caggggtgtg acttgggcct tttcagcgct ccgcgagggt ggggtactgg
     1441 tagggtcaag gccacgccgc gcctgcggtg tgcctggttt cgcatggact cttctcgacg
     1501 ccccaccgac cggcgctcga ccagccaaga cg
//
LOCUS       DAORNF010000815         1527 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_57449_length_1527_cov_1.901495, whole genome shotgun sequence.
ACCESSION   DAORNF010000815 DAORNF010000000
VERSION     DAORNF010000815.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57449_length_1527_cov_1.901495"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>276
                     /locus_tag="PLV68_21710"
     CDS             <1..>276
                     /locus_tag="PLV68_21710"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HRE03927.1"
                     /translation="DALGLGGEPITTLGDEIDRWERAFTTVSGLPDASRAAEVIGALR
                     ASVPPGIPARIIHGDFRLGNMLASGAHLNAVVDWEIWGRSDPRLDLA"
     assembly_gap    277..289
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <290..542
                     /locus_tag="PLV68_21715"
     CDS             <290..542
                     /locus_tag="PLV68_21715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03928.1"
                     /translation="QAHPTAIRDHPGMPTGRQLIAEYTAGGGSAVDHLPWFRSLALTK
                     MAASTALIAKHNMRVGLTNTAEKTAVTVAPMLQGALNAL"
     gene            622..>1527
                     /locus_tag="PLV68_21720"
     CDS             622..>1527
                     /locus_tag="PLV68_21720"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRE03929.1"
                     /translation="MTLRLRHFGTTDMRVTNIGIGASALGGGDWALGWGDQDDADSIA
                     CIHRALEAGINWIDTASLYGLGHSEDVVARALHGIPDADRPYVFTKCGIGWDPYDRSK
                     APWSDCSADAIKRCVEGSLRRLRVDRLDLCQIHWPAADGTPLEEYWGAMLELRAEGKI
                     RAAGLSNHGVTRMKQAEALGHVDSLQPPFSAIKRGAAAELLPWCAAHGVATIAYSPME
                     GGLLTGAFSHERVASLSNDDQRRLLPEYTTELGGNLQIAAAMAVVAASRGVPTPAIAV
                     AWTLSFPALTGSIVGARTPAQVDGWL"
BASE COUNT          242 a          544 c          498 g          230 t
ORIGIN      
        1 ccgacgcgct cggactcggc ggcgagccga tcacaaccct gggcgacgag atcgatcgat
       61 gggagcgggc gttcaccacg gtgtccggcc tacccgacgc ctcccgagct gcggaggtga
      121 tcggcgcact ccgggcgagc gtgccaccag gcatccccgc tcgcatcatc cacggcgact
      181 tccggctcgg gaacatgctc gccagcggtg ctcacctcaa tgccgtcgtc gactgggaga
      241 tctgggggcg gtccgacccg cgcctcgacc tcgcctnnnn nnnnnnnnnt caagcccacc
      301 cgacggcgat ccgcgatcac cccggcatgc caaccggtcg gcagctgatc gccgagtaca
      361 ccgccggtgg cggatcagcc gtcgatcacc tgccgtggtt tcggtcgctc gccctcacca
      421 agatggccgc gtcgaccgcc ttgatcgcca agcacaacat gcgggtgggt ctgacgaaca
      481 ccgccgagaa gaccgcggtg accgtcgccc ccatgctcca gggagccctc aatgcgctct
      541 gatcgtcacg gcgcgccgca ctgctctttc cgatatttca accaatcgat tggtatagtg
      601 aactgacgat cgggggacgc catgacactg cggctgcggc acttcgggac gaccgacatg
      661 cgggtgacca acatagggat cggcgcgtca gccctcggcg gtggcgactg ggcgctgggt
      721 tggggtgacc aggacgacgc cgactcgatc gcctgcattc accgcgcgct cgaagccggg
      781 atcaactgga tcgacaccgc ctcgctgtac gggctcggtc actccgagga tgtcgtcgcc
      841 cgggccctgc acggcattcc agatgccgat cgcccctacg tgttcaccaa gtgcggcatc
      901 gggtgggatc cctacgaccg gtcgaaggcc ccctggagcg actgctcggc cgacgcgatc
      961 aagcgctgcg tcgaggggtc gctgcgccgc ctccgggtcg accggctcga tctgtgccag
     1021 atccattggc ccgcagccga cggcaccccg ctggaggagt actggggagc gatgctcgag
     1081 ttgcgggcgg agggaaagat ccgcgccgcg gggctgtcca accacggcgt gacgcggatg
     1141 aagcaggccg aggcactcgg ccacgtcgac tcgctgcaac caccgttctc ggcgatcaag
     1201 cggggcgcag cggccgagtt gctcccctgg tgcgccgcac acggcgtcgc caccatcgcc
     1261 tacagcccga tggaaggtgg gctgctcacc ggggcgttct cgcacgaacg ggtcgcgtcg
     1321 ctgagcaacg acgatcagcg acgcctcctg cccgagtaca cgacggaact cggcggcaac
     1381 ctgcagatcg ccgccgcgat ggccgtcgtt gccgccagcc gcggcgtccc gacccccgcg
     1441 atcgccgtcg cctggacgct gagctttccc gcattgaccg gctcgatcgt cggtgcccgc
     1501 accccggcgc aggtcgatgg ctggctc
//
LOCUS       DAORNF010000816         1524 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_57593_length_1524_cov_1.363513, whole genome shotgun sequence.
ACCESSION   DAORNF010000816 DAORNF010000000
VERSION     DAORNF010000816.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1524
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57593_length_1524_cov_1.363513"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>236
                     /locus_tag="PLV68_21725"
     CDS             <1..>236
                     /locus_tag="PLV68_21725"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HRE03930.1"
                     /translation="AAAAPVLDHARQVLALTTDLVDWSDRVRGGRRGRVRLGMIDVAF
                     VVHYPDVVAAFRAERPEVDLTLSVAPSANLFAALR"
     assembly_gap    237..421
                     /estimated_length=185
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <422..657
                     /locus_tag="PLV68_21730"
     CDS             <422..657
                     /locus_tag="PLV68_21730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03931.1"
                     /translation="LAAVGAPLTIAAESHQPDVLLQMVRLGLGWTVLPPPSPPPSDVV
                     IGPTLLERVLVLAHRSDSITDPAVTTLADRLRA"
     assembly_gap    704..759
                     /estimated_length=56
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<760..1502)
                     /locus_tag="PLV68_21735"
     CDS             complement(<760..1502)
                     /locus_tag="PLV68_21735"
                     /inference="COORDINATES: protein motif:HMM:NF009057.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HRE03932.1"
                     /translation="MMETFGATVHPSPSELTASGRAILAANPDSPGSLGIAISEAVEV
                     AAPDPSIRYALGSVLNHVLMHQTVIGLEALKQFDLAGEYPDVIYGCVGGGSNFGGLTF
                     PFLAENLAGRRNTRIVAVEPAACPSMTKGVYAYDFGDTAGMTPLMKMHTLGHDFIPDP
                     IHAGGLRYHGMSPLVSHIYELGLIEAESKNQLDCFAAGVQFARTEGIVPAPEPTHALA
                     ACIDEALRCKESGESKVILTALCGHGMLDL"
BASE COUNT          186 a          433 c          462 g          202 t
ORIGIN      
        1 gccgctgccg cgcccgtgct cgaccacgcc cgccaggtgt tggcgctgac gaccgatctg
       61 gtcgattgga gcgaccgcgt acgcggcggc cgccgcgggc gggtgcggct ggggatgatc
      121 gacgtcgcgt tcgtggtgca ctaccccgac gtggtcgccg cgttccgggc cgagcggccc
      181 gaggtcgacc tcacgctgtc ggtcgctccg tcggccaacc tgttcgccgc gttgcgnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 ngcctcgccg cggtcggagc cccgctgacg atcgcggccg agagccacca accggacgtg
      481 ttgttgcaaa tggtacgact cggcctcggt tggaccgtcc tgccgccacc atcacccccg
      541 ccgtccgacg tcgtgatcgg cccgaccctg ctcgaacgcg tgttggtgtt ggcccaccgc
      601 tccgactcca tcaccgaccc ggcagtcacc accctcgccg accgcctccg cgcctgacgc
      661 ggcagagacg gagcatcaca ccttggggag ggcggcgagg gcgnnnnnnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna ggtcgagcat gccgtgaccg
      781 cacagggcgg tgaggatcac cttggactcg ccactctcct tgcagcgcaa cgcctcgtcg
      841 atgcaggcgg cgagggcgtg ggtgggctcg ggggccggga cgatgccttc ggtgcgggcg
      901 aactgcacac cggcagcgaa gcagtcgagc tggttcttcg actcggcttc gatcaggccg
      961 agctcgtaga tgtgcgacac cagcggggac atgccgtggt agcgcaggcc gccggcatgg
     1021 atcgggtcgg ggatgaagtc atggccgagg gtgtgcatct tcatcagcgg cgtcatgccg
     1081 gccgtgtcac cgaagtcgta ggcgtagaca cccttggtca tgctggggca cgccgcaggt
     1141 tcgacggcga cgatgcgggt gttgcgccgc ccggccaggt tctcggccag gaacgggaac
     1201 gtcaggccac cgaagttgct gccaccgccg acgcatccgt agatcacgtc ggggtattcg
     1261 cccgcgaggt cgaactgctt cagcgcctcg aggccgatca ccgtctggtg catcagcacg
     1321 tggttcagca ccgagccgag cgcgtagcgg atgctggggt cgggcgcggc gacctcgacc
     1381 gcttcgctga tcgcgatgcc gagcgagccg ggcgaatctg ggttggcggc gaggatcgcc
     1441 cggcccgacg cagtgagctc ggacggcgac gggtgcacgg tggcaccgaa ggtctccatc
     1501 atcaaccgcc ggtacggctt ggcg
//
LOCUS       DAORNF010000817         1523 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_57627_length_1523_cov_1.586512, whole genome shotgun sequence.
ACCESSION   DAORNF010000817 DAORNF010000000
VERSION     DAORNF010000817.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57627_length_1523_cov_1.586512"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            181..699
                     /locus_tag="PLV68_21740"
     CDS             181..699
                     /locus_tag="PLV68_21740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03933.1"
                     /translation="MVSFLVFAGLIGLCVAMLYAAQRVDPHWVSKDGQRMICQGQGLA
                     RDGTPVTRWRELKVAEVGDGRVEVRTRHGSLTATQPTRQDYSPTAALRRPLFREPRQK
                     TSMWRVQGIAADAPKRKVIYVLDGNDDPGMPALVTLRMPANSKAIPMLDRLLATRVGR
                     IERATPDSTDTA"
     gene            complement(742..>1523)
                     /gene="metX"
                     /locus_tag="PLV68_21745"
     CDS             complement(742..>1523)
                     /gene="metX"
                     /locus_tag="PLV68_21745"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004414 - homoserine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HRE03934.1"
                     /translation="GSRFPVITIRDMVRCQARLADHLGIEVFHSVIGGSMGGMQALEW
                     AITYPKRLRSLVPIATCAQATAQQIAWGAIGRRALLLDEKWRGGDYYGAEPGDGPWRG
                     LAIARMVAQVTFRSDNVFTDRFGRNLADGASLAGGFDLWQRFEVERYLDHHGEKLVRR
                     FDANSYLLIGKAMDLHDVGRGRNGLRSAMARIAVPTLTMGIWSDMLYPAYQQRQIADL
                     ISANGVSSEYVEIDSPHGHDAFLINFDQVARPLARFLDTVG"
BASE COUNT          260 a          511 c          525 g          227 t
ORIGIN      
        1 cgttgcgcgt gctggtcgag tcgttgtctc cgagcagcag ggcaagagtg gccgaccgag
       61 cggacggttg gacggacaga tgaacggcag gactcacgac gacctcatag caagggtgcc
      121 agtctcgcag gtctccgtca cgcgcgtgca gccacccccg ccgcgaggca agctgggggc
      181 gtggtttcgt tcctcgtctt cgccggcctg atcggattgt gcgtggcgat gctgtacgcg
      241 gcccagcggg tggaccccca ttgggtcagc aaggacggcc aacggatgat ctgccaaggc
      301 caagggctcg cccgcgatgg caccccggtc acccgctggc gcgagttgaa ggtggccgag
      361 gtgggcgacg gacgggtcga ggtgcggacg cgccacggca gcctcaccgc cacccagccg
      421 acgcgccagg actactcccc gacagcggcg ttgcggcgac cgttgtttcg cgagccgcgc
      481 cagaagacgt cgatgtggag ggtgcagggc atcgctgcgg acgctccgaa gcgcaaggtg
      541 atctacgtcc tcgacggcaa cgacgatccc ggtatgcccg cgctggtgac gctgcgcatg
      601 cccgccaaca gcaaggcgat ccccatgctc gaccgtttgc tcgcgacccg ggtcgggcgg
      661 atcgagcgcg ccaccccgga ctcaaccgat accgcttgat cagacaacgc gtgatcaggc
      721 gacgactggt caggcgactg gtcagccaac cgtgtcgagg aagcgggcga gcggacgagc
      781 gacctgatcg aagttgatca ggaaggcgtc gtgtccgtgc ggcgagtcga tctcgacgta
      841 ctcggagctc accccattgg cactgatcag gtcggcgatc tgacgctgct ggtacgccgg
      901 gtagagcatg tcgctccaga tgcccatggt cagcgtgggc acggcgatac gagccatggc
      961 agaccgcagc ccgttgcggc cgcggccgac gtcgtgcagg tccatcgcct tgccgatcag
     1021 caggtagctg ttggcgtcga agcggcggac cagcttctcg ccgtggtggt cgagatagcg
     1081 ctcgacctcg aaccgctgcc acagatcgaa cccgccggcc aacgaggccc catcggccag
     1141 gttgcgcccg aagcgatcgg tgaacacgtt gtcgctgcgg aacgtgacct gggcgaccat
     1201 ccgcgcgatc gccagacccc gccacggccc gtcgcccggc tcggcgccgt agtagtcgcc
     1261 gccgcgccac ttctcgtcga gcagcagcgc ccggcgcccg atcgcgcccc acgcgatctg
     1321 ctgtgcagtg gcctgggcgc aggtggcgat cggcaccagc gaccgcaacc gcttcggata
     1381 agtgatcgcc cactccagcg cttgcatgcc gcccatcgag ccgccgatca cgctgtggaa
     1441 cacctcgata ccgagatggt cggccaggcg ggcctggcag cggaccatgt cgcggatggt
     1501 gatgaccggg aagcgcgagc cat
//
LOCUS       DAORNF010000818         1522 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_57683_length_1522_cov_1.419905, whole genome shotgun sequence.
ACCESSION   DAORNF010000818 DAORNF010000000
VERSION     DAORNF010000818.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57683_length_1522_cov_1.419905"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>326)
                     /locus_tag="PLV68_21750"
     CDS             complement(<1..>326)
                     /locus_tag="PLV68_21750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03935.1"
                     /translation="PEGVFFPEMVIDVELLPGVTNTVMGGMGSTEERLANFDRQEVYL
                     PRIPTNALQPVSGTETTVITLTDAASAPQLSEQERAALTLTVQPGSAIGENGQPLENV
                     QIGIST"
     assembly_gap    327..507
                     /estimated_length=181
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<508..>760)
                     /locus_tag="PLV68_21755"
     CDS             complement(<508..>760)
                     /locus_tag="PLV68_21755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03936.1"
                     /translation="TFAWLFFTTPMPGASQVTLHVDGSKIRGAADGRFLDADGDGTAG
                     GTLTQRFTTVSRSAVIGTTLVGKVVDPGPDLEPMSFDDIR"
     assembly_gap    761..900
                     /estimated_length=140
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<901..>1151)
                     /locus_tag="PLV68_21760"
     CDS             complement(<901..>1151)
                     /locus_tag="PLV68_21760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03937.1"
                     /translation="GTADATGSALTQLSYRIDGGSSRSLIFDASGAFDEALTYGDLDV
                     GSHALVLTARDAAGNLATLTRTLVVDALAPLAVTSLTPT"
     assembly_gap    1152..1224
                     /estimated_length=73
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1225..>1522)
                     /locus_tag="PLV68_21765"
     CDS             complement(<1225..>1522)
                     /locus_tag="PLV68_21765"
                     /inference="COORDINATES: protein motif:HMM:NF037015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HRE03938.1"
                     /translation="TSDTQAPAIVLDDTPAAINANLTLTGQVLDNLSGVASAQFQIDD
                     GALQALVLDASGGFTVTTTLATDGSADGAHTITIVARDAAGNANAGVTRGFVLDT"
BASE COUNT          157 a          385 c          394 g          192 t
ORIGIN      
        1 cgtgctgatg ccgatctgca cgttctccag cggctggcca ttctcgccga tcgcgctgcc
       61 cggttgcacg gtcagcgtca gcgccgcgcg ctcctgttcg ctgagttgag gtgccgaagc
      121 ggcatcggtc agcgtgatca ccgtcgtctc ggttccgctc accggctgca gggcattggt
      181 cgggatgcgc ggcaggtaga cctcctgccg gtcgaaattg gccagccgct cctcggtcga
      241 gcccatgccg cccatcaccg tgttggtcac tcccggaagc agctcgacat cgatgaccat
      301 ttccgggaag aacacgccct cgggtgnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      481 nnnnnnnnnn nnnnnnnnnn nnnnnnncgg atgtcgtcga aactcatcgg ctccagatcc
      541 gggcccgggt cgaccacctt gccgaccagc gtcgtcccga tcaccgccga gcggctcacc
      601 gtcgtgaagc gctgcgtcag cgtaccgccc gccgtaccgt cgccgtcggc gtcgaggaag
      661 cggccgtcgg ccgcaccgcg aatcttcgat ccatcgacgt gcaacgtcac ctgtgaagcg
      721 cccggcatcg gggtggtgaa gaacagccag gcgaacgtgc nnnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      901 ggtcggcgtc aggctggtca ccgccagcgg cgccagcgcg tcgaccacga gcgtgcgcgt
      961 cagcgtcgcg aggttgccgg ccgcgtcgcg ggccgtgagc accagcgcgt gactgccgac
     1021 gtcgagatcg ccataggtga gcgcttcgtc gaacgccccg ctggcgtcga agatcaggct
     1081 gcggctgctc ccgccgtcga tgcgatagct gagttgcgtc agcgcgctgc cggtggcatc
     1141 ggccgtgccg gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnggtgtc gagcacgaag ccgcgcgtca cgccggcgtt
     1261 cgcgttaccg gcggcatcgc gggcgacgat cgtgatcgtg tgcgcgccat cggcgctgcc
     1321 gtcggtcgcc agcgtggtgg tgacggtgaa gccgccactc gcatcgagaa cgagcgcctg
     1381 cagcgcgccg tcgtcgatct ggaactgtgc cgaggcgact ccggagaggt tgtcgagcac
     1441 ctgcccggtc agcgtgaggt tggcgttgat tgccgccggg gtgtcgtcga gcacgatcgc
     1501 cggcgcctgc gtgtcgctcg tc
//
LOCUS       DAORNF010000819         1518 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_57855_length_1518_cov_1.116883, whole genome shotgun sequence.
ACCESSION   DAORNF010000819 DAORNF010000000
VERSION     DAORNF010000819.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57855_length_1518_cov_1.116883"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>276
                     /locus_tag="PLV68_21770"
     CDS             <1..>276
                     /locus_tag="PLV68_21770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRE03939.1"
                     /translation="ELLSALTGLGYEEPTPIQRETIPLVIAGNDLVGQAATGTGKTAA
                     FALPILQKIVTDDRPGAPQALVLVPTRELAVQVSEALYKYGRELGAR"
     assembly_gap    277..496
                     /estimated_length=220
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <497..>1518
                     /locus_tag="PLV68_21775"
     CDS             <497..>1518
                     /locus_tag="PLV68_21775"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRE03940.1"
                     /translation="EILDSVPDARQTVLFSATLPPRINAMVRRYLKDPLKIQIGLGDS
                     KPEKSLIRQTVYVVQRAYKAAALGRILDVEAPKAAIVFCRTRTEVDQLTETLNGRGYR
                     AEALHGGLSQESRDKVMARLRAGTTELLIATDVAARGLDIDLLTHVVNFDVPSAPESY
                     VHRIGRVGRAGREGVAITLAEPREQRLITNIERLTKHKIAVEKVPTVADLRAVQIEHT
                     TANVREALAGDDLDRFDAVLGALGDEHELREIALAAIKLVHEAGGAVDDDKEIPDALA
                     RFDRNRGERGDRGDRGGQGGRFERNDRSQGDRRGGDRDFPRDNGETGHVYVGLGRQSG
                     VRPGDL"
BASE COUNT          225 a          431 c          450 g          192 t
ORIGIN      
        1 ctgagctgct cagcgcgctg accgggctcg gctacgaaga gccgaccccc atccagcgcg
       61 aaaccatccc cttggtgatc gcgggcaatg acctggtcgg ccaggcggcc accggcaccg
      121 gcaagacggc ggcgttcgcg ctgccgatcc tgcagaagat cgtcaccgat gatcgccccg
      181 gtgccccgca agccctcgtg ctggtgccga cccgagaact cgcggtgcag gtgtccgagg
      241 cgctgtacaa gtacggccgc gaactggggg cgcgggnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      481 nnnnnnnnnn nnnnnnagag atcctcgatt cggtgcccga cgcgcgccag acggtgctgt
      541 tttcggccac gctgccgccg cgcatcaacg cgatggtgcg ccgctatctg aaagacccac
      601 tcaagatcca gatcggtctg ggtgactcca agcccgagaa gtcgctgatc cgtcagacgg
      661 tctacgtggt gcagagggcc tacaaggccg ctgcactggg tcgcatcctc gacgtcgagg
      721 cgccgaaggc ggcgatcgtg ttctgccgca cacgtaccga ggtcgaccag ctcaccgaga
      781 cgttgaacgg gcgtgggtat cgggccgagg cgttgcacgg cgggctcagc caggagagcc
      841 gcgacaaggt gatggcccgc ctgcgggctg gcaccaccga actcttgatc gccaccgatg
      901 ttgccgcgcg cggcctcgac atcgacctgt tgacccacgt cgtcaacttc gacgtgccgt
      961 ctgcgcccga gtcgtacgtg caccgcatcg gccgcgttgg ccgagccggg cgcgaaggtg
     1021 tcgcgatcac gttggccgag ccgcgcgagc agcgcctgat caccaacatc gagcgcctca
     1081 ccaagcacaa gatcgcggtc gagaaggtgc ccacggtggc cgacctgcgc gcggtgcaga
     1141 tcgaacacac cacggccaac gtgcgagagg cgcttgctgg cgacgacctc gatcggttcg
     1201 atgccgtact cggcgcattg ggcgacgagc acgaactgcg tgagattgcg ctcgccgcga
     1261 tcaagctggt gcacgaagcc gggggagccg tcgacgacga caaggagatc cccgacgcgc
     1321 tggcgcggtt cgatcgcaac cgtggcgagc ggggagaccg tggggatcgg ggcggccagg
     1381 gcggtcgttt cgaacggaac gatcgctctc agggcgaccg acgcggcggc gatcgcgact
     1441 tcccgcgtga caacggtgag accgggcacg tctacgtcgg ccttggccgt cagagcggcg
     1501 tccgtcctgg tgatctcg
//
LOCUS       DAORNF010000820         1517 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_57889_length_1517_cov_1.392613, whole genome shotgun sequence.
ACCESSION   DAORNF010000820 DAORNF010000000
VERSION     DAORNF010000820.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57889_length_1517_cov_1.392613"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="PLV68_21780"
     CDS             complement(<1..333)
                     /locus_tag="PLV68_21780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03941.1"
                     /translation="MVAALALGQHGFPLGVHAQHDRWKERHDVERAPLPVTGAWRPGD
                     PVGHRQFLTIAVDHPMALDGGTTLTDIVVAYETWGTLNADASNAILVCHAWTGDSHLA
                     GASGPGHVD"
     gene            complement(472..>901)
                     /locus_tag="PLV68_21785"
     CDS             complement(472..>901)
                     /locus_tag="PLV68_21785"
                     /inference="COORDINATES: protein motif:HMM:NF024591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HRE03942.1"
                     /translation="KTAQTFRVYEGRRWSVPGDFAEVAADGTLRLLGRGSVCINTGGE
                     KVFPEEVEEALKTHPAVADAVCVGLPDERFGQTICAVIEPVPGSVPDLGTLSAHVRST
                     LAAYKAPRSLVVVDSIMRAPNGKVDYKRLTALAEASATLG"
     assembly_gap    902..911
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<912..>1278)
                     /locus_tag="PLV68_21790"
     CDS             complement(<912..>1278)
                     /locus_tag="PLV68_21790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HRE03943.1"
                     /translation="QAFAGPMLDELRAHPGRYDLGSVALITSSGVMWSQENKTGLLEF
                     MPNAILFDSLGSSEAVGLGASLSTKGAPQATAKFMLGPNCAVFDDDGNRVEPGSAEIG
                     RVAVGGFIPLGYYKDEAKTA"
     assembly_gap    1279..1296
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          214 a          529 c          515 g          231 t
ORIGIN      
        1 gtcgacgtgg cccgggccgc tggccccggc cagatggctg tcgccggtcc aggcgtggca
       61 gacgaggatc gcgttcgacg cgtcggcgtt cagcgtgccc cacgtctcgt aggcgacgac
      121 gatgtcggtc agcgtcgtgc cgccgtcgag cgccatcggg tgatcgaccg cgatcgtgag
      181 gaactggcga tggccgaccg gatcaccggg ccgccaggcc ccggtcaccg gcaacggtgc
      241 tctctccacg tcgtgtcgtt ccttccatcg gtcgtgctgc gcgtgcacac cgagcgggaa
      301 gccgtgttgc cccagcgcga gcgctgcgac cacattcctg ctggcaccga agcgtcagcc
      361 ggaggcacct tgaggcttca gtcccaggtt gccgggccac ccgggtgagt ggccgctcgt
      421 catgcgtggc gcagtgtacc gctccccgcg cggcgacccg cggagattcc gtcagcccag
      481 ggttgcgctc gcttcggcca gcgcggtgag gcgcttgtag tcgaccttgc cgttgggggc
      541 gcgcatgatc gagtcgacca ccacgagcga cctcggcgcc ttgtacgcgg cgagcgtgga
      601 ccgcacgtgg gcgctgagcg tgccgaggtc ggggaccgaa ccgggcaccg gctcgatgac
      661 ggcgcagatc gtctggccga agcgttcgtc gggcagaccg acacagaccg cgtcggcgac
      721 ggccggatgg gtcttcaacg cctcctcgac ctcttccgga aacaccttct cgccgccggt
      781 gttgatgcac accgagccgc ggcccagcaa ccgcaacgtg ccgtcggccg cgacctcggc
      841 gaagtcgccc ggaacgctcc accggcggcc ctcgtagacc cggaacgtct gggccgtctt
      901 gnnnnnnnnn nagcggtctt cgcctcgtcc ttgtagtagc cgagcgggat gaacccgccg
      961 accgcgaccc ggccgatctc ggccgaaccc ggctcgacgc ggttgccgtc gtcgtcgaac
     1021 accgcgcagt tcggcccgag catgaacttc gcggtcgcct ggggggcgcc tttggtggag
     1081 aggctggcgc cgaggccgac cgcttcgctg ctgccgagcg agtcgaacaa gatcgcgttg
     1141 ggcatgaact cgagcagacc ggtcttgttc tcctggctcc acatcacgcc gctggaggtg
     1201 atcagcgcga cgctgccgag gtcgtagcgg ccggggtgcg cgcgcagctc gtcgagcatc
     1261 gggccggcga aggcctgann nnnnnnnnnn nnnnnnccga tcgaccgtct ccaacagctc
     1321 ggtgacgctg aacgcccgac tggccagcga cacgatgcag ccacccccca tcatcgcgtt
     1381 gatcgccgag aactggccgg tgccgtgcat cagcgggcag gccaccaaca ccccggtgcc
     1441 gcgcggtgcg ctggtgtcga tgcgcgccac catctcttcc accgaccccg ccggtgggac
     1501 accggtgatc gggctgc
//
LOCUS       DAORNF010000821         1505 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_58403_length_1505_cov_1.735172, whole genome shotgun sequence.
ACCESSION   DAORNF010000821 DAORNF010000000
VERSION     DAORNF010000821.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58403_length_1505_cov_1.735172"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="PLV68_21795"
     CDS             <1..245
                     /locus_tag="PLV68_21795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03944.1"
                     /translation="GRLRPAMQGVIKSYDPATGEGVLVCDTDLNDYDLAADALAGSIL
                     RMVRQGQRVVFRLDEQGRATELRLGSEVDMGTPGAH"
     assembly_gap    268..348
                     /estimated_length=81
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            410..>1505
                     /locus_tag="PLV68_21800"
     CDS             410..>1505
                     /locus_tag="PLV68_21800"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HRE03945.1"
                     /translation="MSGTSDNQRLHQWVNEWAEILQPSDIYWCDGSAEEYDRLAQQLV
                     DAGVFTKLDEAKRPNSYWGHSDQGDVARVEDRTFICSENEADAGPNNNWRDPAEMRAE
                     LKRLYAGAMKGRTLYVVPFSMGPLGSPIAHIGVQLTDSAYVATNMRIMTRMGQGALDV
                     LGANGEWVPCIHSVGAPLEPGQADSAWPCNPDNKYIVHFPETREIMSFGSGYGGNALL
                     GKKCFALRIASVMARDDGWLAEHMLILKLTSPAGEHKYIAAAFPSACGKTNLAMLVPT
                     IPGWKAETIGDDICWMKYGADGRLYAINPEAGFFGVAPGTGYDTNANAMETLWGNSIF
                     TNTALTDDGDVWWEGMSDTKPARATDWRGNA"
BASE COUNT          264 a          477 c          468 g          215 t
ORIGIN      
        1 aaggcaggct gaggcccgcg atgcagggcg tcatcaagtc ctacgacccg gcaacgggtg
       61 agggtgtcct cgtctgcgac accgatctca acgactacga cctggccgcc gatgcgctgg
      121 ccgggtcgat tctgcgcatg gtccgccagg gccagcgcgt cgtgttccgg ctcgacgaac
      181 aggggcgcgc caccgagctg cgcctcggta gtgaagtcga catgggcacc cccggcgcgc
      241 actgatctcg ctgctggcgc acgcgccnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncg gttcacgaat
      361 cgccggggcc catttggatc acgcgacctc gacgggaaag actgacggca tgagtggcac
      421 cagtgacaac cagcggcttc atcaatgggt gaacgagtgg gcggagatcc ttcagcccag
      481 cgacatctac tggtgcgacg ggagtgcaga ggagtacgac cggctcgcac agcaactcgt
      541 cgacgccggt gtgttcacca agctcgacga ggccaagcgt cccaacagct actggggcca
      601 cagcgaccag ggtgacgtcg cccgggtcga ggatcgcacc ttcatctgct ccgagaacga
      661 ggccgacgcc ggccccaaca acaactggcg tgacccggcc gagatgcggg ccgagctcaa
      721 gcgtctgtac gccggcgcga tgaagggtcg caccctgtac gtggtgccgt tcagcatggg
      781 tccgctcggt tcgccgatcg cccacatcgg cgtgcagttg accgattcgg cctacgtggc
      841 caccaacatg cggatcatga cccgcatggg ccagggtgcg ctcgatgtgc tcggcgccaa
      901 cggcgaatgg gtgccgtgca tccactcggt cggcgcaccg ctcgaaccgg gtcaggccga
      961 ctcggcgtgg ccgtgcaacc ccgacaacaa gtacatcgtc cacttccccg agacgcgcga
     1021 gatcatgtcg ttcgggtctg gctacggcgg caacgcgctg ctgggcaaga agtgcttcgc
     1081 cctgcggatc gcttcggtca tggcccgcga cgacggctgg ctggccgagc acatgctgat
     1141 cctcaagctc accagccccg cgggcgagca caagtacatc gctgccgcgt tcccgtcggc
     1201 gtgcggcaag accaacctgg ccatgctcgt gccgacgatt ccgggttgga aggcagagac
     1261 catcggtgac gacatctgct ggatgaagta cggcgccgac ggtcgcctgt acgcgatcaa
     1321 cccggaggcc gggttcttcg gcgtggcccc cggaaccggc tacgacacca acgccaacgc
     1381 gatggagacc ctgtggggta acagcatctt caccaacact gcgctcaccg acgacggtga
     1441 cgtgtggtgg gaaggcatga gcgacaccaa gcccgcccgc gccaccgact ggcggggcaa
     1501 cgcct
//
LOCUS       DAORNF010000822         1504 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_58446_length_1504_cov_1.706694, whole genome shotgun sequence.
ACCESSION   DAORNF010000822 DAORNF010000000
VERSION     DAORNF010000822.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58446_length_1504_cov_1.706694"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>265
                     /locus_tag="PLV68_21805"
     CDS             <1..>265
                     /locus_tag="PLV68_21805"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HRE03946.1"
                     /translation="ASNNATPVQVTQAELAVKNNELALRDAQLSLERRSIVAPIGGIV
                     GILPVEVGNYITNQTAVATIDDRSQIIVDFWVPERYAAAMAVGA"
     assembly_gap    266..297
                     /estimated_length=32
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <298..>651
                     /locus_tag="PLV68_21810"
     CDS             <298..>651
                     /locus_tag="PLV68_21810"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRE03947.1"
                     /translation="AFDGTVSAVDNRIDDQSRTLWVQARIANPNDTLRAGMSFEVTMK
                     FAGDTYPAVDPLAIQWGNDGAYVWVVADGKAKRTPVKIVQRNTDSVLVQAALTPSEQV
                     VTEGVQTVREGAAVRV"
     assembly_gap    652..714
                     /estimated_length=63
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            741..>1114
                     /locus_tag="PLV68_21815"
     CDS             741..>1114
                     /locus_tag="PLV68_21815"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRE03948.1"
                     /translation="MTDRSEHPEAGLTALFIRRPVLAFVLNVLIAVAGMAAFYGVEIR
                     ELPDVDRPVITVTTSYNGAAAETIDREITAVVEGAVSRVSGVKSISSSSSFGRSRVTV
                     EFGDGVDLNVAASDMRDAVGRV"
     assembly_gap    1115..1141
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1142..>1504
                     /locus_tag="PLV68_21820"
     CDS             <1142..>1504
                     /locus_tag="PLV68_21820"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRE03949.1"
                     /translation="PRIVKADSNADAVIRLAVTSDAMSVEDMTILVEDQVLDTLAAVD
                     GVADVQTYGDRNKVFRIDIDQAKVASHGLTLGDLANALATVAFDAPAGSLISGDQNIV
                     VRATANVTTPDQFESIVIN"
BASE COUNT          249 a          464 c          460 g          209 t
ORIGIN      
        1 ggcctccaac aacgccacgc cggtgcaggt gacccaggcc gaactggcgg taaagaacaa
       61 cgaactcgcg ttgcgcgacg cgcaattgtc gctggaacgc cgctccatcg tggcgccaat
      121 tggcggcatc gtcggcatcc tgccggtcga ggtcggcaac tacatcacca accagacggc
      181 ggtcgcgacc atcgacgacc gctctcagat catcgtcgat ttctgggtgc cggagcgcta
      241 tgcggcggcc atggccgtcg gcgcgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngca
      301 ttcgacggca cggtgagcgc cgtggacaac cgcatcgacg accagagcag gacgctgtgg
      361 gtgcaggccc gcatcgccaa tccgaacgac acgctgcgcg ccggcatgtc gttcgaggtg
      421 acgatgaagt tcgccggcga cacctatccg gcggtcgatc cgctggcgat ccagtggggc
      481 aatgacggcg cctatgtctg ggtggtcgcg gacggcaagg ccaagcgcac gcccgtgaag
      541 atcgtccagc gcaataccga cagcgtgctg gttcaggcag cgctgacgcc atcggagcag
      601 gtggtgacgg aaggtgtgca gacggtgcgc gagggcgcgg ccgtccgcgt tnnnnnnnnn
      661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncggcag
      721 cgggacatga cagtcgcaac atgaccgacc gttccgaaca tccagaagcc ggcctcaccg
      781 ccctgttcat acggcggccg gtgctggcct tcgtgctcaa cgtgctgatc gcggtggccg
      841 gcatggcggc cttctacggc gtcgaaatcc gcgaactgcc ggacgtcgac cggccggtga
      901 tcaccgtcac caccagctac aatggcgccg cggcggagac catcgaccgc gagatcaccg
      961 ccgtggtcga gggcgcggtg tcgcgcgtct ccggcgtgaa gtcgatctcg tcgagctcgt
     1021 ccttcgggcg aagccgcgtc acggtggaat tcggcgacgg cgtcgacctg aacgtcgccg
     1081 cttcagacat gcgcgatgcg gtcggccgcg tgcannnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nccgcgcatc gtgaaggccg attccaacgc cgacgccgtg atccggctgg cagtgacctc
     1201 cgacgccatg tcggtggagg acatgaccat cctggtcgag gaccaggtgc tggacacgct
     1261 ggccgccgtt gacggcgtcg cggacgtgca gacctacggc gaccgcaaca aggtgttccg
     1321 catcgacatc gaccaggcca aggttgcaag ccacggcctg acgctcggcg acctagccaa
     1381 cgcgctggcg accgtcgcct tcgacgcccc ggccggctcg ctgatcagcg gtgaccagaa
     1441 catcgtcgtg cgcgccaccg ccaacgtgac cacgccggat cagttcgaat ccatcgtcat
     1501 caac
//
LOCUS       DAORNF010000823         1504 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_58451_length_1504_cov_1.555556, whole genome shotgun sequence.
ACCESSION   DAORNF010000823 DAORNF010000000
VERSION     DAORNF010000823.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58451_length_1504_cov_1.555556"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="PLV68_21825"
     CDS             complement(<1..251)
                     /locus_tag="PLV68_21825"
                     /inference="COORDINATES: protein motif:HMM:NF024248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRE03950.1"
                     /translation="MPDQDVLRQSRALGDPTRYAVFAYLRDAPGGAGVAELTEHFGLN
                     HNAIRQHLAKLVAASLVGALSEQLGDRPVKIKLEHVLVET"
     gene            complement(475..945)
                     /locus_tag="PLV68_21830"
     CDS             complement(475..945)
                     /locus_tag="PLV68_21830"
                     /inference="COORDINATES: protein motif:HMM:NF012674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body protein"
                     /protein_id="HRE03951.1"
                     /translation="MPTLSTSSLLVASRTALLAKMLGVDMVSQNLANVNTYGYRGQRM
                     NFQELLNDRTNINGVQPVATQILLAPGRLETTGRSLDLAVDGDRKRDAILGDERGERV
                     GRHHIVVLENAVQTEHLHLIAPEAALHALGGGDAVADAAGAQHLEDLDQHDLAA"
     gene            complement(945..>1224)
                     /locus_tag="PLV68_21835"
     CDS             complement(945..>1224)
                     /locus_tag="PLV68_21835"
                     /inference="COORDINATES: protein motif:HMM:NF018168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar basal body rod C-terminal
                     domain-containing protein"
                     /protein_id="HRE03952.1"
                     /translation="DNPPFATLGLAAFNDPRTELVGDLPNTYSAAAAPTGTDIGQVVQ
                     GYLEDSNVNPTLMTAQMVEVNRAYEAAQKLVQTQDELLGRVISTLGRL"
     assembly_gap    1225..1263
                     /estimated_length=39
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1264..>1504)
                     /locus_tag="PLV68_21840"
     CDS             complement(<1264..>1504)
                     /locus_tag="PLV68_21840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03953.1"
                     /translation="LGDWRSIRDTIGALRQTEEPLDVAIEGPGFFTIETPNGVRYTKD
                     GRFLRDAENNLVTSEGYFVLDDAGQRITLPETGTLV"
BASE COUNT          243 a          470 c          479 g          273 t
ORIGIN      
        1 gtctccacga ggacgtgctc gagtttgatc ttcaccggcc ggtcgccgag ctgttcggac
       61 agcgcgccga ccagcgacgc cgcgaccagc ttggccaggt gctgacggat cgcgttgtgg
      121 ttgaggccga agtgctcggt caactccgcg accccggcgc cgcccggagc atcgcgcaga
      181 taggcgaaca ccgcgtaccg tgtcgggtca ccgagcgccc ggctctggcg cagcacgtcc
      241 tgatcgggca agcgctgatc cggggggcga tcagttgggg catcggcacg ggccatcgca
      301 gctcctcggt cacgagcgat cgaataatcc tagcgatcac ctgagaaact tgttgaacgt
      361 cacgacgatc ggagcaccat catgaccgct cacacgaatc caccacatcc cggcgacctg
      421 atgacactcg acgtgtcggg ttggctcaac agcgacccca tcgatctcgc cagcctacgc
      481 ggcaaggtcg tgctgatcga gatcttccag atgctgtgcc ccggctgcgt cagccacggc
      541 atccccaccg ccaagcgcgt gcagcgcggc ttcgggcgcg atcaggtgga ggtgctcggt
      601 ctgcacagcg ttttcgagca ccacgatgtg atgacgcccg acgcgctcgc cgcgttcgtc
      661 tccgagtatc gcatcacgtt tccggtcgcc atcgaccgcc aggtcgagcg agcgccctgt
      721 ggtctcgagc cggcccgggg ccagcaagat ctgcgtggcg acgggctgga cgccgttgat
      781 gttggtccgg tcgttgagca gctcctggaa attcatgcgc tggccccgat agccataggt
      841 gttgacgttg gccaggttct ggctgaccat gtcgacgcca agcattttgg cgagcagcgc
      901 ggtgcgtgaa gcgacgagca gcgaagacgt ggagagcgtc ggcatcacag cctcccgagg
      961 gtggagatga cccgcccgag cagttcgtcc tgcgtctgca cgagcttctg ggccgcctca
     1021 taagcacggt tgacttcgac catctgggcc gtcatcagcg tcgggttgac attcgaatcc
     1081 tcgagatatc cctgcacgac ctgtccgatg tctgtccctg tcggggcagc cgccgcgctg
     1141 taggtattgg gcagatcgcc caccagctcg gtccgcgggt cgttgaaggc cgcgaggcca
     1201 agcgtggcaa acggcgggtt gtcgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnncacgagc gtgcctgttt cgggcagcgt gatgcgctgg cccgcgtcgt cgagcacgaa
     1321 gtagccttcg cttgtcacga ggttgttttc ggcatcccgg agaaatcgcc catctttggt
     1381 gtagcggacg ccgttcgggg tctcgatcgt gaaaaagcct gggccctcga tcgccacgtc
     1441 gaggggctcc tcggtctggc gaagcgcgcc tatcgtgtcc cggatactgc gccagtcgcc
     1501 tagg
//
LOCUS       DAORNF010000824         1504 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_58459_length_1504_cov_1.415459, whole genome shotgun sequence.
ACCESSION   DAORNF010000824 DAORNF010000000
VERSION     DAORNF010000824.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58459_length_1504_cov_1.415459"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>349
                     /locus_tag="PLV68_21845"
     CDS             <1..>349
                     /locus_tag="PLV68_21845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRE03954.1"
                     /translation="LISPDSSRKRIQHLEGGILRELLVPILPRTVVLWLLRLGLIGAV
                     VLHIHAAYGLTIINRKARAVKYQGERDYQVANFASRTMRWSGVIIALFVVWHLADLTW
                     GTTTALGTDGTFVR"
     assembly_gap    350..431
                     /estimated_length=82
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            605..>809
                     /locus_tag="PLV68_21850"
     CDS             605..>809
                     /locus_tag="PLV68_21850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRE03955.1"
                     /translation="MTLTASDPRLDSKIPDGPMADKWDNYKSAMKLVNPNNKRKFKVI
                     VVGSGLAGASAAATMAELGYQVDV"
     assembly_gap    810..825
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <826..>1117
                     /locus_tag="PLV68_21855"
     CDS             <826..>1117
                     /locus_tag="PLV68_21855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRE03956.1"
                     /translation="HSIAAQGGINAAKNYPGDGDSIHRLFYDTVKGGDFRAREGNVYR
                     LAQVSVNIIDQMVAQGVPFAREYGGLLDNRSFGGAQVSRTFYARGQTGQQLLL"
     assembly_gap    1118..1298
                     /estimated_length=181
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1299..>1504
                     /locus_tag="PLV68_21860"
     CDS             <1299..>1504
                     /locus_tag="PLV68_21860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HRE03957.1"
                     /translation="NVFFLSTNAMNCNVTAAWRAHRRGAMFANPCYTQIHPTCIPQSD
                     PTQSKLTLMSESLRNDGRVWVPKE"
BASE COUNT          216 a          435 c          374 g          200 t
ORIGIN      
        1 ttgatttcgc cggacagcag ccgcaagcgc atccagcacc tggaaggcgg catcctgcgc
       61 gaactgctgg tgccgatcct gccccgcacc gtcgtgctct ggctgctgcg gctcgggttg
      121 atcggtgccg tggtgctgca catccacgcc gcgtacgggc tgacgatcat caaccgcaag
      181 gcccgcgccg tcaagtacca gggcgagcgc gactaccagg tggccaactt cgccagccgc
      241 accatgcgct ggtcgggcgt catcatcgcc ctgttcgtcg tctggcacct ggccgacctc
      301 acctggggca ccaccacggc gctcggcacc gacggcacgt tcgtgcgtgn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn ntcttccacg gcatgtggag cctgttccag agcctgggca tcaacagccc
      481 ccggttcaac gcgtggcgtc gccacttcgc caccgccatc gccgccatcg tggtgatcgg
      541 caatgtctcg ttccccgtcg ccgtgctgac cggcatcgtc ggctgagtcg aaagaagcac
      601 ccacatgact ctcaccgcat ccgacccgcg tctcgacagc aagatccccg acggcccgat
      661 ggccgacaag tgggacaact acaagtcggc gatgaagttg gtcaacccca acaacaagcg
      721 caagttcaag gtcatcgtcg tcggtagcgg cctggccggg gcgtcggccg cagccacgat
      781 ggccgaactc ggttatcagg tcgacgtgtn nnnnnnnnnn nnnnncccac tcgatcgccg
      841 cccagggcgg catcaacgcc gccaagaact atcccggcga cggcgacagc atccaccggc
      901 tgttctacga cacggtcaag ggcggcgact tccgcgcccg tgaaggcaac gtgtaccgcc
      961 tggcccaggt cagcgtcaac atcatcgacc agatggtcgc gcagggtgtg cccttcgctc
     1021 gtgagtacgg cggtctgctc gacaaccgca gcttcggcgg ggcccaggtg agccggacgt
     1081 tctacgcccg cggccagacc ggtcagcagc tcttgctnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnca acgtcttctt cctctccacc
     1321 aacgcgatga actgcaacgt caccgcggcg tggcgggctc accgccgggg ggcgatgttc
     1381 gccaacccgt gctacaccca gatccacccc acctgcattc cgcagagcga tcccacgcag
     1441 tcgaagttga cgctgatgag cgagtcgctg cgcaacgacg ggcgggtgtg ggtgccgaag
     1501 gagg
//
LOCUS       DAORNF010000825         1503 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ilumatobacteraceae bacterium isolate AMR_MDS_5454
            NODE_58519_length_1503_cov_1.103591, whole genome shotgun sequence.
ACCESSION   DAORNF010000825 DAORNF010000000
VERSION     DAORNF010000825.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867671
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ilumatobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Ilumatobacteraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Ilumatobacteraceae.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:36:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 4,313
            CDSs (with protein)               :: 4,313
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 9 of 26
            Pseudo Genes (incomplete)         :: 16 of 26
            Pseudo Genes (internal stop)      :: 2 of 26
            Pseudo Genes (multiple problems)  :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Ilumatobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58519_length_1503_cov_1.103591"
                     /isolate="AMR_MDS_5454"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2497619"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    211..477
                     /estimated_length=267
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<478..952)
                     /locus_tag="PLV68_21865"
     CDS             complement(<478..952)
                     /locus_tag="PLV68_21865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRE03958.1"
                     /translation="MDITDASAIVTGGASGIGAATARLLSERGAKVVIADLQEDRGRA
                     LADDIGGVFAKLDVTATDTIIAAVELAKDIGPLRVLVNSAGVGSAQRTVGRDGTYASA
                     ASLEAYSRIIAINLIGTFDCIRLAATAMSTTPALEHGERGAIVNLASVAAFDGQIG"
     assembly_gap    1101..1233
                     /estimated_length=133
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1234..>1503)
                     /locus_tag="PLV68_21870"
     CDS             complement(<1234..>1503)
                     /locus_tag="PLV68_21870"
                     /inference="COORDINATES: protein motif:HMM:NF027439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRE03959.1"
                     /translation="VAAVEGLALAGGLEIVLMCDLVVAARGARLGIPETQVGLFAAGG
                     GLYRLGQRLPAPVAAEMALLGTPISAEEGHRLGLVNRLADAGTALD"
BASE COUNT          150 a          382 c          394 g          177 t
ORIGIN      
        1 tgcaccaggt cgtgccggtg gaactgactc gcgacgaggc ctgcgcgata ctcgccgacc
       61 tggtcacccg ttggctgacc taccctgtcc ccggcgacgt accggacgct gggagcatca
      121 cggcactcgt gacgtcaagg acctgatccg cctgcgcctc cgtgggccgg gtggagcacg
      181 gtcacttcgg ttgcatgcga atgccgccgt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngcc
      481 cgatctgtcc gtcgaaggcg gcgaccgacg cgaggttgac gatcgctccc cgctcgccgt
      541 gttcgagcgc tggggtggtg ctcatcgccg tggccgccaa ccgaatgcag tcgaacgtgc
      601 cgatcaggtt gatcgcgatg atgcgggaat acgcctccag gcttgctgcg ctggcgtagg
      661 tcccgtcgcg cccgacggtg cgctgcgcgc ttccgacgcc cgctgagttg accaggacgc
      721 ggagcgggcc gatgtctttg gccaactcca ccgccgcgat gatcgtgtcg gtcgccgtaa
      781 cgtcgagctt ggcgaagacc ccaccgatgt cgtcggcgag ggcccgcccg cggtcctctt
      841 ggaggtcggc gatgacgacc ttcgcgccgc gctcgctgag cagcctcgcc gtcgccgcac
      901 cgattccaga tgcgccgccg gtgacgatgg cgctggcgtc tgtgatgtcc atgggggtct
      961 cctgtgtcgg ggggtgcgat gagtgaagcg tgggcgagcc gggccggcgc ggccaacgac
     1021 gccgtgcggt gttaccgagc ggtccagcgc ggcgggcggc gttcgcgaaa ggccgtcgcc
     1081 ccttcgtggg catcggccga nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngtcgagc gcggtgcccg cgtctgccaa
     1261 gcggttgacg aggcctaacc gatgaccctc ctcggcgctg atcggcgtgc ccaacagggc
     1321 catctccgca gcgaccggtg ctggcagtct ctggccgagg cggtagaggc cgccgccggc
     1381 cgcgaagagc ccgacctggg tctccgggat ccccagtcgc gcgcctcgcg cggcgacgac
     1441 caggtcgcac atgagtacga tctcgaggcc gccggccagg gccaagccct cgaccgctgc
     1501 gac
//
